LOCUS       DBNGVD010000001         7517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4372615, whole genome shotgun sequence.
ACCESSION   DBNGVD010000001 DBNGVD010000000
VERSION     DBNGVD010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4372615"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="AC16CM_00005"
     CDS             complement(<1..737)
                     /locus_tag="AC16CM_00005"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138238.1"
                     /translation="MIEFRGVSKTYPGSEKPVVRDLSFEVLDGEICVLVGPSGCGKTT
                     SMRMVNRLIEITEGEILIDGDPNTSMSGTQLRRKIGYAIQQIGLFPHRTIADNIGTVP
                     NLLGWEKGRIKERVDELLDLVGLDPGVYRDRYPAELSGGQQQRVGVARALAADPPIML
                     MDEPFGAVDPITRERLQDEFLRIQEDIKKTIVFVTHDIDEAIKMGDKIAILKEGGILA
                     QYDTPARILSHPDSEFVSSFVGADRVL"
     gene            complement(944..1216)
                     /locus_tag="AC16CM_00010"
     CDS             complement(944..1216)
                     /locus_tag="AC16CM_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138239.1"
                     /translation="MGLIRTILALVIVLILVHVGLVYLDIRPGTNVLTNAVYSLGVLV
                     ESPAQLLLATVGPLRQYLNPNNFYVVALTAAAVYFILYLLVGVGRR"
     gene            complement(1547..1915)
                     /locus_tag="AC16CM_00015"
     CDS             complement(1547..1915)
                     /locus_tag="AC16CM_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138240.1"
                     /translation="MGFLLTAIGFLLLVTSLGGIAIGVYMALDKSTRRRGQLFAIVWV
                     PAVAASSGVMMHDVVTFTVGLVCFLIAGTVFVLQGDQPYEPPADTRADPARDPAGDRF
                     LASEKTTKENKTRSRGRIAS"
     gene            complement(1925..2338)
                     /locus_tag="AC16CM_00020"
     CDS             complement(1925..2338)
                     /locus_tag="AC16CM_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR3138241.1"
                     /translation="MPLAASYIRTDSDGALPTREAQEAEISAFADASGYELVAGYEGL
                     EAPGLLLYYRPGIKAAINNIKELEEWEVLLISDPACISDTESALHEFVHKLSLYGNRL
                     ESPDRSWEEFLAAMKSYRRAMGRTSQERETDDGVR"
     gene            2398..2471
                     /locus_tag="AC16CM_00025"
     tRNA            2398..2471
                     /locus_tag="AC16CM_00025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2432..2434,aa:Pro,seq:ggg)
     gene            2776..3504
                     /locus_tag="AC16CM_00030"
     CDS             2776..3504
                     /locus_tag="AC16CM_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138242.1"
                     /translation="MDLGCLILHEAHDESRLIRLRDRMKAEGEQRNPVIVSPHEDRYL
                     VLDGAHRVHALEELGSPLALVQMVEVPDDAEGWGHLLGGLKTAELGSLRGVEASEEPG
                     PNPLAEVEADGVGTVFLSSTGEGLRDLVSALWSLQSLYPKGVVHRVEPRAAVSLPEGG
                     AIIRYRGFTPKELAAIVDSGAVLPAGITRFRIEERVLGVNYPLKGMSGGDLDSLNDQL
                     GEYVRRRWNEGRVRFYGEPVVLFE"
     gene            3848..5155
                     /locus_tag="AC16CM_00035"
     CDS             3848..5155
                     /locus_tag="AC16CM_00035"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR3138243.1"
                     /translation="MSEAQQNGTQHAGEEPIIRVENCSVVFGRRAKQALEMRRSGKSK
                     AEVESATGSTIGAYEATFDVYPGEIFVIIGLSGSGKSTILRAVNGLNEPTEGSVHLDG
                     DHISGMSSKQLREVRRKKIGMVFQHFGLLPNRTVLDNIGFGLEIKGVSFQERKKKAEE
                     TLPAVGLEGQGEKLISELSGGMKQRVGLARALATEQEILLMDEPFSALDPLIRRDMQN
                     LFLSIQGEAQRTVIFVTHDLDEALTLGHRAAIMKDGEIVQIGTPEQILREPATEYVER
                     FVEDVDYAKVRMAESVMVEPKEVAYTSEGPTVVLRRMRQAGLSHIFVVDGERRFVGLA
                     EAEEVARLAERDERELGAAVDKEAPSVERRTPLREVLPSFLDDSDAPVAVVEEDGRLA
                     GGIVRGSLIAGLTTSAEVPEDREEVPSGSDDRAHSEVGQEGGR"
     gene            5158..6015
                     /locus_tag="AC16CM_00040"
     CDS             5158..6015
                     /locus_tag="AC16CM_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138244.1"
                     /translation="MVALLQDIPQIPLAKWINGIIFWVIDNFGGFFSALSSLIEGVVG
                     GISFILSTPPALVMVAVFTVVAFFLAGWRIALFTVIGLLLVISLGLWELTMTTLALIL
                     VATIVCLAIGIPIGIIAAKSPRLEEFILPVLDLMQTMPAFVYLVPFVFLMNLGAAPAL
                     LATVIFATPPAVRLTMLGIQQVPSETVEAAHAFGSTFRQTLFKVELPLSLSSIRAGIN
                     QVIMLSLSMVVIAGLVGFEGLGKPVVEGLQQLNVGLAFEGGIGIVVLAIILDRVTRGI
                     GAGRRAKKA"
     gene            6061..6990
                     /locus_tag="AC16CM_00045"
     CDS             6061..6990
                     /locus_tag="AC16CM_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3138245.1"
                     /translation="MDLRGKLTRRDLLKLSGGALAGASLLGVAGCGSGGGSSSSGSKT
                     LTLGNIGWTENVAISNLTKVVRGEELDYDVSLKGPLDLGPMFQGLANNDIQAFQDVWL
                     PNNQNYLDKPQIKPRVELLSPWFQGTTKYGITVLSYMEGITSIADLNDAGTSEITGIE
                     AGASFMPVIEDKVIPEYNLDMKLVTSSTAAMLAEVQKKNAAKEPIVFTGWSPHWMNTK
                     YDIVYLEDPKNAQGAFDDPSKITSVVNGGLKDEDPMAYAFLKAIKVNEDQLNEMEAEM
                     VAAGTGNEEKGVKKWYMKNKDLVNPWVDAAKKA"
     gene            7197..>7517
                     /locus_tag="AC16CM_00050"
     CDS             7197..>7517
                     /locus_tag="AC16CM_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3138246.1"
                     /translation="MSRKRFLGLGGASLAGMAMLGAAGCGGGEGGGGPVGITFTFGPE
                     ASGSLQGLIDKFNKEHKGEIKVTWRTAAAASDDYFDQIKTQLQAGEATVDVIGGDVIW
                     PAQFA"
BASE COUNT         1437 a         2190 c         2489 g         1401 t
ORIGIN      
        1 ttgaggaccc tgtcagcgcc gacgaaggag gagacgaact cggagtctgg atgggagagt
       61 attctggccg gggtgtcgta ctgggccagg atgccaccct ccttgaggat ggcgatcttg
      121 tcgcccatct ttatcgcctc gtcgatgtcg tgggtgacga agacgatggt cttctttatg
      181 tcctcctgaa tccgcaagaa ctcgtcctgg agcctctcgc gggtgatcgg gtcgacggcc
      241 ccgaacggct cgtccatgag catgatgggg gggtcggccg cgagagcccg cgccaccccg
      301 acccgctgct gctgcccgcc ggagagctcc gccgggtaac ggtcgcgata gacgccggga
      361 tcgaggccca cgaggtcgag tagctcgtct acccgctcct tgatgcgccc cttctcccag
      421 cccagcaggt tcggaaccgt acctatgttg tccgctatgg tgcggtgggg aaagaggcct
      481 atctgctgga tggcgtagcc gatcttgcgc ctgagctgcg taccgctcat ggaggtgttc
      541 ggatcgccgt cgatgaggat ctcgccctcg gtgatctcga tcaaacggtt gaccatccgc
      601 atggacgtgg tcttgccgca accgctcggc cccaccaaca cacagatctc cccgtcgagc
      661 acctcgaagg agagatctct gaccaccggc ttttcagagc cgggataggt cttgcttacg
      721 cccctaaact cgatcattgc acctcctgcg ggcccagacg tgccattcct tatccccgtt
      781 catacgcacc cttatttcgt cgaccatggc tcctctccgg acctgcaaaa attcgttggc
      841 aaagtatacg tgaatatcgt aggcatacta gtgagcgtcc cggctcgccc cagggaacgc
      901 tgtcccgaac gtgcagatca ggatgttggt ccggcaggcc tggttagcgg cggccaacac
      961 cgaccaggag gtagaggatg aagtacaccg cggcggcggt gagggcgacc acgtagaaat
     1021 tgttcgggtt gaggtactgt cgcaaaggcc ccacggtagc gagcagcaac tgggcgggtg
     1081 actcgaccag caccccgagg ctgtatacgg cgttggtcag tacgttggtg ccaggcctga
     1141 tgtccaggta taccagcccg acgtgaacga gtatgagcac gatgaccagg gcgagtatcg
     1201 tgcggatgag gcccatcctc tgtctccttc cgtgcgcggc caacgccgtt gcgccccgga
     1261 ctcccccgcg agcctcccaa gggcctttct cgccggccag tatagcaagt cgcttcgcgc
     1321 gaatttaatg aagatcagcg aaatgatcga gctggtgtgg agaggagggc atcaagctac
     1381 aggtttttta ctgatgcttg ttgcaattct tcacccagag ttcacttttt ccagagcgcg
     1441 aaaggatgaa tacgggtgag gctttgtgag gggcgatcga atttcctgac ccggtcctgg
     1501 aagctttcat tcacgtggcc actctgcgcg gcggccgcgc gagccgtcag gaagcgatcc
     1561 tgccccggct cctcgtcttg ttttcctttg tggtcttttc ggaggctagg aacctgtccc
     1621 cggcgggatc cctggcaggg tcggccctcg tgtccgctgg cggttcgtac ggctggtcac
     1681 cttgcaggac gaagacggta ccggcaatca ggaagcagac caggccgacg gtgaaggtta
     1741 cgacatcgtg catcatgacg ccgctggatg cggccacagc cggcacccag acgatagcga
     1801 agagctggcc gcgtcggcgg gtgctcttgt cgagggccat gtagacgccg atcgcgatgc
     1861 ctccaaggga ggtcaccagc aagagaaacc ctatggcggt cagcaagaag cccatcagca
     1921 tatcctaccg gacaccgtcg tcggtttcac gttcctgtga tgtccggccc atggctcgcc
     1981 ggtaagactt catggcggcg aggaactcct cccaggagcg gtcgggggac tcgagccggt
     2041 ttccgtacag ggatagtttg tgcacgaact cgtgtagcgc cgattccgtg tcggagatac
     2101 aggcagggtc ggagatcagg agcacctccc actcctcgag ctcctttatg ttgtttatgg
     2161 cagccttgat gccgggcctg tagtagagga ggagacccgg ggcttcgagg ccctcgtagc
     2221 ccgctaccag ctcgtagccc gacgcgtcgg cgaaggccga gatctcggcc tcctgcgcct
     2281 cccgggtggg gagagcgccg tcggaatcgg tccttatgta ggatgcagcc agcggcaagg
     2341 gaaaccgatg atagcacggg cttcgtctgg tatgatcgag gctgtcaaga cacgcgtcgg
     2401 gacgtggcgc agtctggtag cgcactcggc tgggggccga gtggtcgccg gttcaaatcc
     2461 ggccgtcccg actgttcgcg gtcagaccgc acaccggcat cacttgaaag gctagccttg
     2521 gacacactct acctgcttat gggcatcata gggttcttcc tgatcatcgc gctgttcttc
     2581 ttcggcgggt tcgtcgtgct cgattttctc tggggccggc gtgggggaga tctctagggc
     2641 actcccttct ggagccgctc tagaaagacg cgaactcgaa gtggggcaag gcatcaggct
     2701 acaggctaca ggcatcaggt tttcactcct gaagcctaat tccgcatgat cccttccctg
     2761 aggagcttgc ggctggtgga cctagggtgc ttgatcctgc acgaggccca cgatgaaagc
     2821 cggctgattc ggctcaggga ccgcatgaag gcggagggcg agcaacgaaa ccccgtgatc
     2881 gtttccccgc acgaggaccg ctacctcgtc ctcgatggcg cccacagggt gcacgccctc
     2941 gaagagctcg gctctcccct ggccctggtc cagatggtcg aggtgcccga cgatgctgag
     3001 gggtggggac acctgctagg tggcctgaag acagcggaac ttggcagcct tcggggcgtc
     3061 gaagcctcgg aggaacccgg ccccaacccg ctggcagagg tggaggccga cggcgttggc
     3121 accgtctttc tctcctcgac cggggagggg ttgcgggatc tggtaagcgc cctgtggtcc
     3181 ctgcagtccc tctatccgaa gggcgttgtt catcgcgtgg agccccgggc cgccgtaagc
     3241 ctccccgagg gtggggccat catccgctac agagggttca cgccgaagga gctggccgcc
     3301 atcgtggatt cgggggccgt gctgcccgcc ggcataaccc gattccggat agaggaacgc
     3361 gtgctcggcg tcaactaccc actgaaaggt atgtcgggcg gagatctgga ctcgctgaac
     3421 gatcaactcg gggaatacgt gcgccggcgc tggaacgagg gcagggtacg gttctacggg
     3481 gaacccgtcg tgcttttcga gtaagcagct ccctatccgg atttcgccag cgtcagagcc
     3541 aggcttaccg gctcataccc gaaatgctag aattgggccg tattgcggct tgatcgcatt
     3601 atcctgggtt ccttcgcctt ctggtgatca gccgggcggg acctgcggca tctgtgctgc
     3661 gctggtctcg ggatcgcgtt cgctcgagcg tcttcccata gaaagagcaa gacatgtttc
     3721 tgtacttggg gtgacagggt ggggtcagag atgatcccgg atgaggccgg gcacgtacaa
     3781 gaacgtaccc ggtaacacga gcggctctta tgatgccgtt tgtgccgggg ctaggaggta
     3841 ggaagacgtg agcgaggcac agcagaacgg tacacagcac gctggcgagg agccgatcat
     3901 ccgggtcgag aactgctcgg tggtcttcgg gcggagggcg aagcaggccc tggagatgag
     3961 gcgttcgggc aagagcaagg ccgaggtaga gagcgcaaca gggtcgacga tcggggccta
     4021 cgaggccacc ttcgacgttt acccgggtga gatcttcgtg atcatcggcc tatccgggag
     4081 cgggaagtct acgatcctgc gcgccgtcaa cggcctcaac gagccgaccg aggggagcgt
     4141 ccacctcgac ggcgaccaca tctcggggat gtcgtccaag cagctcaggg aggtaaggcg
     4201 caagaagatc gggatggtct tccagcactt cgggttgctg cccaacagga ccgtcctgga
     4261 caacatcggg ttcgggctgg agatcaaggg cgtctccttc caggagcgca agaagaaagc
     4321 ggaggagacc ctgcccgcgg tcggcctcga ggggcagggt gagaagctca tctccgagct
     4381 ttcgggcggc atgaagcagc gcgtcgggct cgcccgggcg ctcgcgaccg agcaggagat
     4441 actgctcatg gacgagccgt tctccgcgct cgacccgctc atcaggcgcg acatgcagaa
     4501 cctttttctg agcatccagg gcgaggcaca gcgcacggtc atcttcgtga cccacgacct
     4561 cgacgaggcg ctcacgctcg ggcaccgggc ggcgatcatg aaggacgggg agatcgttca
     4621 gataggcacc ccggagcaga tactgcgcga gcccgcgacc gagtacgtgg aacgcttcgt
     4681 ggaggacgtg gactacgcca aggtgcgtat ggccgagagc gtgatggtcg agccgaaaga
     4741 ggtggcctac acgagtgagg gaccgacggt cgtcctgcgc aggatgaggc aggcaggcct
     4801 ctcccacatc ttcgtggtcg acggagagcg gcgcttcgtc ggcctcgccg aggccgagga
     4861 ggttgccagg ctcgccgagc gagacgagag ggagcttggg gccgccgtcg acaaagaggc
     4921 gccgtctgtg gagcgaagga cgccgcttcg ggaagtactt ccctctttcc tggacgacag
     4981 cgacgctccg gtcgccgtcg tcgaagaaga tgggcgtctg gcaggcggca tcgtcagggg
     5041 ctcgctcata gccgggctca ccacgagcgc cgaggtgccg gaagatcgtg aggaagtccc
     5101 ctcaggctcc gatgaccgcg cccacagtga ggtcggacaa gagggggggc ggtagcgatg
     5161 gtggcgttgt tgcaagacat cccgcagata ccgctcgcca agtggatcaa cgggataatc
     5221 ttctgggtca tcgataattt cgggggcttt ttcagcgcgc tgagcagcct gatcgagggc
     5281 gtcgtcggcg gcatctcctt catcctgtca acgccccccg cgctggttat ggtggccgtc
     5341 tttaccgtgg ttgctttctt cttggcggga tggcggatag cgctctttac ggtgatcggg
     5401 ttgttgctcg tcatcagcct cgggttgtgg gagcttacga tgacgaccct cgccctcatc
     5461 ctcgtcgcca cgatcgtgtg cctggcaata gggataccga tcgggataat cgccgccaag
     5521 tcgccccggc tggaggaatt tattctcccc gtactggacc tgatgcagac catgccggcc
     5581 ttcgtgtatc tggtcccgtt cgttttcttg atgaacctcg gtgcggcgcc agccctgctc
     5641 gcgacggtca tttttgccac gccgcccgcc gtgaggctga ccatgctggg catacagcag
     5701 gttccgagtg agacggtgga ggccgcgcac gccttcggat cgacgttcag gcagaccctc
     5761 ttcaaggtgg agctaccgct ctcgctgagc tcgatcaggg ccggaatcaa ccaggtcatc
     5821 atgctctcgc tctcgatggt ggtaatcgcc ggcctggtcg gcttcgaggg gctcggtaag
     5881 ccggttgtag aggggttgca gcaactcaac gtcgggctgg ccttcgaggg cggtattggt
     5941 atcgtggtcc tcgcgattat tctggacagg gttacccggg gtatcggagc aggaagaagg
     6001 gcgaaaaagg cgtaagggcg gagaccgaaa gcgaactgga agcaaatcta ggaggaaagc
     6061 gtggatttga gaggcaaact aaccagaagg gatttgctca agctaagtgg tggcgcgctg
     6121 gcaggcgcca gccttctcgg ggtggcgggc tgcggcagcg gcggcggctc aagctcctcg
     6181 ggcagcaaga cacttaccct gggtaacatc ggctggaccg agaacgtcgc catctccaac
     6241 ctgaccaagg tcgtgagggg tgaggagctc gactacgacg tgtcgttgaa gggacccctg
     6301 gacctcggac cgatgttcca gggtctggcc aacaacgata ttcaggcgtt ccaggacgtg
     6361 tggttgccca acaaccagaa ctatttggac aagccccaga tcaagccgcg cgtggagctc
     6421 ttgagcccgt ggttccaggg gaccaccaag tacggcatca cggtgctctc ctacatggag
     6481 ggcataacga gcatcgccga cctcaacgac gccggcacaa gcgagatcac cggcatcgag
     6541 gccggcgcct ccttcatgcc cgtgatcgag gacaaggtta tccccgaata caacctggac
     6601 atgaagctcg tcacctccag cacggccgcc atgctcgccg aggtacagaa gaagaacgcg
     6661 gccaaggaac cgatagtgtt cacgggctgg tccccgcact ggatgaacac caagtacgac
     6721 atcgtctacc tggaggaccc gaagaacgcc cagggcgcct tcgatgaccc ctccaagatc
     6781 accagcgtgg tcaacggtgg tctgaaggac gaggatccca tggcctacgc cttcctgaag
     6841 gccatcaagg tcaacgaaga ccaactcaac gagatggaag cggagatggt cgcggctgga
     6901 acgggcaacg aggagaaggg cgtcaagaag tggtacatga agaacaagga cctcgtgaat
     6961 ccctgggtcg acgccgcgaa gaaggcctga gctagccggg tgacacttcg gcccgccggc
     7021 accccgttgc cggcgggcca ggcacaccca ttccgtaatg cgagttgaaa agttcgttgc
     7081 gcgcccgccc gcaccgattt gccggtgggc gcgttctggt ctatgtttgc agtgggtagg
     7141 cgaggaaggg ctggactatg cctgatatgg ggacgtccgt atccaccgga cgcgggatga
     7201 gccgaaaaag gttcctggga ttgggcgggg cgtcgctggc cggcatggcg atgctcgggg
     7261 ccgccgggtg cggcggcggc gaaggagggg gtggcccggt agggatcacc ttcaccttcg
     7321 ggcctgaggc ctcggggagc ctgcaggggc tcatagacaa gttcaacaag gagcacaaag
     7381 gcgagataaa ggtaacctgg cggaccgccg ctgcggcgag cgacgactac ttcgaccaga
     7441 taaagacgca gctccaggct ggagaggcca cggtggacgt tattggtggc gacgtgatct
     7501 ggccggccca gttcgcc
//
LOCUS       DBNGVD010000002         5627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4008361, whole genome shotgun sequence.
ACCESSION   DBNGVD010000002 DBNGVD010000000
VERSION     DBNGVD010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4008361"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /gene="selA"
                     /locus_tag="AC16CM_00055"
     CDS             complement(<1..1289)
                     /gene="selA"
                     /locus_tag="AC16CM_00055"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3138247.1"
                     /translation="MMETGNRDRLRGLPAVDAVLRGRPGIALADRHGRARATGAVREA
                     LDDLRRRISAGEKPEVSEKAVVDAALDLLAGRGLVRVVNATGVILHTNLGRSVLSERA
                     VLAVSEAATGYSNLEYDLRSGGRGSRYDHAVPLLRELTGAEDALVVNNCAGATLLALS
                     AIVASGASEAPEVIVSRGQLIEIGGGFRIPEVLELSGSRLREVGTTNRTRLSDYEGAI
                     NENTRAILWVHPSNFETVGFTESVPVSDLATLGLPVVADVGSGALLPVGGEPRVQDAV
                     GQGADLTIFSGDKLLGGPQAGIAVGEERWISAMRRHPLARALRADKLCLAALEATLSS
                     YLDGTAEGELPTLRTLETPAQLLEGRAANLAGALRSNTPALEVDVTPSVARSGGGTLP
                     TYEIPSFAVRLGGTDAEALAADLRASDPPVIGRVHEG"
     gene            1393..1686
                     /locus_tag="AC16CM_00060"
     CDS             1393..1686
                     /locus_tag="AC16CM_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138248.1"
                     /translation="MGEGTSKFVLGATIGAVGGFVVGTFATTPTARSARQSAFGGVNL
                     SARFLGRTVVRAVDGLGSVAESGYTRIRGREKYLEHEIDDLREQIARLQQKID"
     gene            complement(1852..2193)
                     /locus_tag="AC16CM_00065"
     CDS             complement(1852..2193)
                     /locus_tag="AC16CM_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138249.1"
                     /translation="MERNMESDPAQTPASLGYEFAREEEARTAAADLDRRFTETELSG
                     LRIYRVRWNGNYLVEATFSPDTPEPRLHDASALLGEAGTPVHPDDLEDYKRATDRTNA
                     LPTWLRRFFRD"
     gene            complement(2183..3202)
                     /locus_tag="AC16CM_00070"
     CDS             complement(2183..3202)
                     /locus_tag="AC16CM_00070"
                     /inference="COORDINATES: protein motif:HMM:NF021608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR3138250.1"
                     /translation="MISWGAARTIAVTLASRTERGPAGDFDYAGAVGATLDPLSRFTG
                     IELPQGPPNGRQLRIADRAEWIDFNIEGFGQLMEPIVQRAAAGAGDFTWAVGGVTLTA
                     QVGLLLGFLSSRVLGQYDTGPLLTPGRDGEPGKVFFLDGNIVSAATRLGVPVDGLRLW
                     IVLHEMTHALQFEGYPWLRRHLGDLLESLISPLADRLGVRETLSRLSTNLKTGGRSVE
                     LLMSRPQRESFDRMQAAMSVIEGYSDYVMHHVGRNLVPNYEHLKERMSRSRSHRPPLE
                     TAVFRLTGLDVKLEQYKLGEAFADTVVKREGMEGLNRIWASPENLPTLAEVRDPGLWM
                     SRLGT"
     gene            3308..3523
                     /locus_tag="AC16CM_00075"
     CDS             3308..3523
                     /locus_tag="AC16CM_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3138251.1"
                     /translation="MFGVGPQEMVIVGLIFLIIFGPSSLPRMAKDMGRFVAEARRAID
                     DFKDEITAAGEDDDDDEGVDDKKERDL"
     gene            complement(3867..4769)
                     /locus_tag="AC16CM_00080"
     CDS             complement(3867..4769)
                     /locus_tag="AC16CM_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR3138252.1"
                     /translation="MRASFLATDTTVRNDSRGVPYLSLKLVDRTGSVDARMWGKLPEE
                     FLGEVPAPAYVAVEGHTHEYRGTLQVKLERLRLLRPEDVAEEDFLPATELDRMDLAGE
                     LEEAGRNLENGHLRDLFEVMVSDEDFWRRFCEAPAAKSMHHARVGGLLEHSVSCLRIA
                     RHLADLYPVDRDLLVFGAIFHDVGKVQELSWDKGAFAYTTEGRLLGHVVLGERLVSTY
                     IDALPDFPEDLRLRISHTLIAHQGETEYGSPERPKTLEALLVHLIDNLDARSAMYMET
                     TTNVSPGGWSHHENPLHRALYVPE"
     gene            complement(4831..5625)
                     /locus_tag="AC16CM_00085"
     CDS             complement(4831..5625)
                     /locus_tag="AC16CM_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3138253.1"
                     /translation="MFLVLFVLVSLFPLLWVFRMSTVTKSDLLTSPAVTLTASPVGTS
                     YSTVFSNPDFIQALQNSVIVAGTTTVICLFFGSICAYSIARLRFRFKGSVMTIILAIS
                     FFPAVAIIAPLFIQFTAVGIINTYWAMIIPDVVFALPLTIWILVAFFRELPRDLEEAA
                     KVDGATTLQAFRKVIVPLAAPGVFTTAILTFIHAWNEFLFANTFIFDAAHYPVTVVIP
                     NFATTYTTDFGAQAAGAVVVSIPLVILVLIFQRRIVSGLTAGAVKG"
BASE COUNT         1009 a         1779 c         1872 g          967 t
ORIGIN      
        1 cttccttcgt gcacgcgccc gatcacgggc gggtcggagg cgcgcaggtc agcggccagg
       61 gcttcggcgt ccgttccccc gagccttacc gcgaaggagg gtatctcgta cgtcggcagc
      121 gttcccccgc cgctacgggc cacggaaggg gtcacgtcga cctccagcgc gggtgtgttg
      181 ctcctcaacg ccccggcgag gttggcggcc ctcccctcca gaagttgggc tggggtctct
      241 agcgtgcgga gggtgggtaa ttctccttcc gcggtaccgt ccaggtagga gctcagggtc
      301 gcctccaggg cggcaaggca gagcttgtcg gctcggaggg cgcgggcgag agggtgccgg
      361 cgcatcgcgg agatccaacg ctcttcgccc accgctatcc ccgcctgcgg ccccccgaga
      421 agcttgtcgc cggagaagat ggtgaggtca gccccctgcc ccacggcgtc ctggaccctg
      481 ggctcgccac ccacgggcaa cagcgccccg ctgccaacgt cggccactac cggaagcccc
      541 agggtcgcga ggtccgagac cggcaccgac tccgtgaagc caaccgtctc gaagttgctc
      601 gggtggaccc agaggatcgc gcgcgtattc tcgtttatgg ccccctcgta gtccgaaagc
      661 ctggtgcggt tcgtggttcc tacctccctg aggcgggagc cggagagttc cagcacctcc
      721 gggatgcgga agccgccccc tatctctatg agctgcccgc gtgagacgat aacctcagga
      781 gcttcggagg caccagacgc aacgatcgcc gagagcgcga ggagcgtggc ccccgcgcag
      841 ttgttaacga ccagcgcgtc ctcggcgcct gtcagctccc tcaaaagcgg caccgcgtga
      901 tcgtagcgcg agcctctgcc tccggaacgg agatcgtatt ccaggttgga gtatcccgtg
      961 gccgcctcgc tcaccgcgag aacggcccgc tcagagagga ccgagcggcc caggttggtg
     1021 tgcaggatca cgcccgtcgc gttcacgacc cggacgagcc ccctgcccgc gagaaggtct
     1081 aaggccgcat ccacgacggc cttctcggac acctccggct tctcaccagc ggagatcctg
     1141 cgccgcagat catcgagcgc ctcgcgtacg gcgcccgttg ccctggcccg gccgtgccgg
     1201 tctgccagcg caatccccgg gcggccgcgc aacacggcgt ctacggcggg gaggccgcgg
     1261 agcctgtccc tattccccgt ctccatcatg cgtggctcag tataacccgg cgtgtcccgc
     1321 cgaaacgtga gccacaagcg ctgtgctaaa cttgcgccgt cgaagcccga aagcgccgaa
     1381 aggaccctga ccttgggcga gggaaccagc aaattcgtgc tcggggccac catcggggcg
     1441 gtgggtggtt tcgtcgtcgg cacgttcgcc acgacaccca ctgcgcgctc ggcccgccag
     1501 tcggcgttcg gaggcgtgaa cctctcagcg cgtttccttg gaaggacggt agtcagggcc
     1561 gtagacggcc tcggctccgt ggccgaatcc ggctataccc gcatccgggg cagggaaaaa
     1621 tacctcgaac acgagatcga cgacctgaga gaacagatag cccgcctgca acagaagata
     1681 gactagcgcg ccggcctccc ctcggggagt ctagcggtca gctttcagca aagttgtccc
     1741 aaagtgacac gtagcgcatc gaatccagcc gctcgggcaa cggatgctcg cactcgggtg
     1801 gccaggtcca ccacgagaaa aagctgaaag ctgatggctg actgctacaa gctaatccct
     1861 gaagaagcgt cttagccagg taggcagggc gttggttctg tctgtggcgc gtttgtagtc
     1921 ttccaggtcg tcgggatgca cgggggtgcc ggcttctcca aggagggcac ttgcgtcgtg
     1981 gagacgtggt tcgggggtat cgggagagaa ggtggcttct accaggtagt tgccgttcca
     2041 tcgtacgcgg taaatgcgca ggccggatag ttctgtctcg gtaaagcgcc ggtccaggtc
     2101 ggcggctgcg gttctggcct cttcctcgcg ggcaaactcg tagccgaggg aggctggggt
     2161 ctgcgcgggg tcgctctcca tgttacgttc caagcctcga catccagagg cccgggtcgc
     2221 ggacctctgc gagcgtgggg aggttctcgg gcgacgccca gatacggttg agaccctcca
     2281 taccctcgcg cttcacgacg gtgtcggcga aggcctcccc aagcttgtac tgctccagtt
     2341 tgacgtcgag accggtcagg cggaacacgg ccgtctcgag aggcggtcgg tggctcctgc
     2401 tccgcgacat gcgctctttc aggtgctcgt agttgggaac gaggttccgg cccacgtggt
     2461 gcatcacgta gtccgaatag ccctctatca cagacatcgc tgcctgcatc ctgtcgaagg
     2521 actcacgctg gggacggctc atcaggagct cgacagatct tccccccgtc ttcaggttcg
     2581 tagagagccg gctcagcgtt tcgcgtaccc cgaggcggtc ggccagaggt gagataaggc
     2641 tctccagaag atccccgagg tgccgccgca gccagggata cccctcgaac tggagcgcgt
     2701 gggtcatctc gtgcagcacg atccagagcc tgagcccgtc taccggcacc ccgagccttg
     2761 tggccgcaga gacgatgttc ccgtcgagga agaagacctt tcccggctct ccatcgcgcc
     2821 ccggcgtgag caacggccca gtgtcgtact gcccaagcac gcgtgaggag agaaagccaa
     2881 gcaagagccc gacctgggcg gtcagcgtta cacccccgac ggcccacgtg aaatcacccg
     2941 caccagccgc agcgcgctgc acgatgggct ccatcagctg cccgaagccc tcgatgttga
     3001 agtcgatcca ctcggcccga tcggcgatcc tgagctgacg tccgttcgga ggtccctggg
     3061 gcaactctat ccccgtaaag cgcgagaggg gatcgagcgt cgcccccacg gcgcccgcgt
     3121 agtcgaagtc gcccgcgggg ccccgctcgg tgcgtgaggc aagcgtaacg gcgatggtac
     3181 gcgccgcccc ccaggagatc acgaacgttc ctcggcgtag agagacatgc gctgagtata
     3241 tacgctgccc gagcctgcga gtttcagccg tttgtaagct gcgcgctccc gggttatatt
     3301 ctgtgccatg ttcggtgtcg gaccccagga gatggttata gtaggcctga tcttcctgat
     3361 tatcttcggc ccgagctccc tccccagaat ggccaaggac atgggccgct tcgtagcgga
     3421 agcccgccgc gccatagacg atttcaaaga cgagatcacc gccgcaggcg aagacgacga
     3481 tgacgacgaa ggcgtggacg acaagaaaga gcgggacctc tagtagggcg cttcgcgccg
     3541 cacgctccgg ctaacgccta cgctcctcag cacactacgg ccttcggcct ccgtttgtca
     3601 gcacgctcgg gctagcgccc tcgctcgtcg gcaacgcata gcttcgcctc gctttcggca
     3661 agttgtatcg gggtggcacg acaagctcgg tcacccctct ttccttcgtg ccgttactcg
     3721 ggggcggcag gctcaggccg tagccttatc agctgaaagc gagcctggcg aagccgggcg
     3781 aagcgttgcc gacgagccgg tgcaagccgg cgtgctgacg aggcgcgaag cgccgtgctg
     3841 aaagcgtagg cgttggccgg agcgtgctac tcgggcacgt agagggcgcg gtggaggggg
     3901 ttttcgtggt ggctccagcc gccggggctg acgttcgtgg tggtctccat atacatggcg
     3961 gacctggcgt cgaggttgtc tatgaggtgg acgaggaggg cttccagggt tttggggcgc
     4021 tcgggggaac cgtattcggt ctctccctgg tgcgcgataa gggtgtggga gatcctgagg
     4081 cgcaggtctt cggggaagtc cggaagggcg tcgatgtagg tggagaccaa tcgctctcca
     4141 agcacgacgt ggccgagaag acggccctcc gtggtatagg cgaaggcgcc cttgtcccag
     4201 gagagctcct gaaccttgcc cacgtcgtgg aagatggcgc cgaagacgag gaggtcgcgg
     4261 tccacggggt agaggtctgc caggtggcgg gcgatcctca ggcagctcac cgaatgctcc
     4321 aacaggcccc cgacgcgcgc gtggtgcatg gatttcgcag cgggagcctc gcagaagcgc
     4381 ctccagaaat cctcatcgga gaccatgacc tcgaagaggt ctcgcaggtg cccgttctcc
     4441 aggttgcgtc ctgcctcctc cagttcgccc gcaaggtcca tccggtccag ctctgtggct
     4501 gggaggaaat cctcttcggc tacgtcctcg gggcggagga ggcgcaggcg ttccagcttg
     4561 acctgcaggg tgccgcggta ttcgtgggtg tggccctcca cggccacgta ggccggggct
     4621 gggacctccc caaggaactc ctcgggtagc ttgccccaca tgcgggcgtc aaccgagccc
     4681 gttcggtcca ccagcttcag ggagaggtag ggcacgccac ggctatcgtt gcggaccgtg
     4741 gtatcggtcg ccaggaagct ggcgcgcaac ctggagccag gctccagatc gcgcgcccag
     4801 aacttctctt tgttgagggg attgttcgtt ctaccctttc acggccccgg ccgtcaggcc
     4861 cgagacgata cgacgctgga agataaggac cagtatcacg agcggaatcg aaacgaccac
     4921 cgcgcccgcg gcctgggccc cgaagtccgt agtgtaggtc gtggcgaagt tcgggatcac
     4981 caccgttacc gggtagtggg cggcgtcgaa gatgaacgtg ttggcgaaaa ggaactcgtt
     5041 ccaggcgtgg atgaacgtca ggatggccgt cgtgaacacg cccggggccg ccagcggcac
     5101 tatcaccttg cggaaagcct gcagcgtcgt cgccccgtcc accttggccg cctcctccag
     5161 gtcacgcggc agttctcgga agaacgccac gaggatccag atcgtcagcg gcagggcgaa
     5221 caccacgtcg gggatgatca tggcccagta ggtgttgatg atacctaccg cggtgaactg
     5281 gatgaacagg ggggctatga tcgcgaccgc cgggaagaag ctgattgcga gtatgatggt
     5341 catgaccgaa cccttgaagc ggaagcggag cctcgctatc gagtaggcgc agatggagcc
     5401 aaagaacagg cagataaccg tcgtcgtgcc ggcgacgatc acgctgttct ggagcgcctg
     5461 gatgaagtcg gggttggaaa agacggtcga gtagctggtc cccacgggtg aggcggtgag
     5521 cgtgaccgcg ggggaggtca ggaggtcgga cttggtgacg gtgctcatcc tgaagaccca
     5581 caagagcggg aacaggctca ctagcacgaa gagcaccagg aacacgt
//
LOCUS       DBNGVD010000003         2183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1458082, whole genome shotgun sequence.
ACCESSION   DBNGVD010000003 DBNGVD010000000
VERSION     DBNGVD010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1458082"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1290
                     /locus_tag="AC16CM_00090"
     CDS             55..1290
                     /locus_tag="AC16CM_00090"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIR3138254.1"
                     /translation="MIPDLVIDAHGVRSGVGVTIEGGRIAGVGPAREGERLSGLALAP
                     GFVNDHSHAFQRGLRGSVERIVPSRPHDDFWSWRERMYDLAGALSPESIREASRRCYG
                     EMLSAGYTSVTEFHYVHHRPDGTPYRDPNALARAVALAAEDAGIRLLLLPVAYARGGL
                     PRFRDPTVQTFLHRVDDLLDWAEGRPMVEVGVAAHSVRAVPRGWLEELADHARGRNLP
                     LHIHADEQEREIEECLAEHGVRPVELLAQTGFLGQSTTIVHATHADGHELDLLAEHGA
                     SVCACPTTEGNLGDGFLPAVGIRDRGIGLSIGSDSHVRVDPFEELREIETNARRTEGR
                     RNVLVNEGEVSPTTYLLRAGWGRAGLRAGDHADLLEVDLWHPSLSGMEEGDLPSTLVF
                     GAGSGIVAGTRVEGERVYG"
     gene            complement(1296..>2183)
                     /locus_tag="AC16CM_00095"
     CDS             complement(1296..>2183)
                     /locus_tag="AC16CM_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR3138255.1"
                     /translation="AFRILRKQFGIFLAHEPGARIGEDPEELHDMRVATRRMRTAMKV
                     FEEALPARARALRNSLKWIMGALGEVRDVDVQLEHLEGWLAEAAPEDKEPLAALRTVL
                     EGQRAKRRKAMLRALDSRRYARLVDSFSTFLRRGPSRRTAGASQPITEAAPRLLRKPF
                     RKVRKLGDPLSEESSGEEFHELRKKGKRLRYALEFFSEIYGQPIREFVGPLKVLQDVL
                     GDHQDAEVAAAHLRELGVDRGRGRKLPPETIFVMGSISHQYALQARELSTRYQKAYRK
                     AVRGKWKKMRGVLEAGPEV"
BASE COUNT          359 a          717 c          717 g          390 t
ORIGIN      
        1 tagcggatct ctgacggttc gcgcgaccag acgccttgtg acgtgaggcg taccttgatc
       61 cctgatctcg taatcgacgc gcacggcgtg cggagcggcg ttggtgtgac gatcgagggc
      121 gggaggatcg ccggggtcgg accggccagg gagggcgaac gtttgtccgg ccttgcgctg
      181 gcacccgggt tcgtaaacga ccacagccac gcttttcagc ggggtttgag gggctcggtc
      241 gaacggatcg taccctcgcg tccccacgat gacttctgga gctggcgcga gcggatgtac
      301 gatctcgccg gcgccctctc acccgaatcc atacgcgagg cgagccgccg ttgttacggg
      361 gagatgctct cggccggcta cacgagcgtc accgagtttc actacgtcca ccatcgcccc
      421 gacggcacac cctaccgaga ccccaatgcc ctggcaagag ccgtggccct ggccgccgaa
      481 gacgcgggga tacggctgct cttgctaccg gtcgcctacg cgcggggcgg cctaccgcgt
      541 ttccgagatc ccaccgtgca aacgtttctg catcgggtcg acgatctgct cgattgggct
      601 gagggacgcc cgatggtcga ggtgggcgtc gcggcacaca gcgtcagggc agtcccgcgc
      661 ggatggttgg aggagctcgc tgatcacgcc cgcgggcgga accttccgtt gcacatccac
      721 gccgacgagc aggagcggga gatcgaggag tgcctcgccg agcacggggt gcggcccgta
      781 gagctgctgg cgcagaccgg cttcctcggt cagagcacca ctatagtcca cgccacacac
      841 gccgacggac acgagctgga cctgctcgcc gaacacggcg cttccgtgtg cgcctgccca
      901 accacggagg gaaacctcgg tgacgggttc ttgcctgccg tggggataag ggacaggggc
      961 atcgggctct ccatcgggtc ggactcgcac gtcagggtgg atcccttcga ggaactgcgg
     1021 gagatcgaaa ccaacgcccg ccgcacggag ggacgcagga acgtcctggt caacgaaggc
     1081 gaggtctcgc cgacgaccta tctgctccgg gcgggctggg gtcgcgcggg gctacgggcc
     1141 ggagaccacg cagacctcct cgaggtggat ctctggcacc cctcgctttc gggcatggag
     1201 gaaggcgatc ttccttcaac gctggtcttc ggtgccggta gcggtattgt tgcggggacc
     1261 agggtagagg gcgaaagagt gtacggctga tccagctaca cttctggccc ggcctccagc
     1321 acgccccgca tctttttcca ttttccgcgc accgcctttc ggtacgcttt ctggtacctg
     1381 gtactcagct cgcgcgcctg taacgcgtac tggtgcgaga tactacccat gacgaagatg
     1441 gtctccgggg gcaactttcg cccgcggccc ctgtcgacgc ctagctccct caggtgggcg
     1501 gcggcgacct cggcgtcctg atgatcgccg agtacatcct gaagcacctt gagcggaccc
     1561 acgaactctc ttatgggctg gccgtagatc tccgagaaga actcgagagc gtagcgcaga
     1621 cgcttgccct tcttgcgcag ctcgtggaac tcttcaccgg aggattcctc cgagaggggg
     1681 tctccaagct tccttacctt gcggaagggt ttgcgtagca ggcgtggtgc cgcctccgtt
     1741 atcggttggc tggcgcccgc ggttctccgc gaaggacctc gacgcaagaa ggtggagaac
     1801 gagtcgacaa gcctcgcgta ccgccgcgaa tcgagcgcac ggagcatggc cttgcgccgc
     1861 ttcgcgcgtt gcccttcgag cacggtgcgc agcgccgcta gcggctcctt gtcttccggt
     1921 gcggcctcgg ctagccagcc ctccagatgc tcgagctgca cgtctacgtc ccgcacctcc
     1981 cccagggcgc ccatgatcca cttgagcgag ttgcggagag cccgcgcccg cgccggcaag
     2041 gcctcctcga agaccttcat agcggtccgc atccggcgag tcgcgacccg catgtcgtgc
     2101 agttcctcgg ggtcttcacc gatcctggcg ccaggctcgt gggccagaaa gatgccgaac
     2161 tgctttctaa ggatcctgaa ggc
//
LOCUS       DBNGVD010000004         6616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_729575, whole genome shotgun sequence.
ACCESSION   DBNGVD010000004 DBNGVD010000000
VERSION     DBNGVD010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_729575"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="AC16CM_00100"
     CDS             <1..1593
                     /locus_tag="AC16CM_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3138256.1"
                     /translation="PDPQPVRDLHEELVEPSIEPTREIEVVLPERRPAEAFDQERELG
                     PGEYTPPAFSLLGLGTGDPEHDAEGTSRRLTEALSDLGVEAHVVRAVVGPRVTRYEMR
                     LGSGVKVGKVRNLQQDIAYALAATEVRILAPIPGKSAVGVEVPNTRPAKVTLGDVFQS
                     YPDANDWTLPVGLGKDISGRAVFFDLSEMPHLLVAGTTGSGKSVMLNGLLTSLLLTTD
                     PRQVKMVLIDPKRVELSQFGRVPHLITPVVTDVKKAANALTWGVAEMERRYEVLERNG
                     VRSLEGYNTKAEVPMPYVVVVIDELADLMMQAGAKVEDAIIRLAQKARAVGIHLVVAT
                     QRPSVDVITGMIKANVPSRIAFAVSSQVDSRVIMDNPGAEALLGMGDMLYKPVSAARP
                     SRVQGAFISEAEVERVVSATVEAASGRAEARFVEEVTEPRNVKEENDEPDDDLLPDAA
                     SFVVSTQQASVSAVQRRFRVGYSRAGRIIDALERKGVVGPYEGSKSRAVVASPMDLET
                     LFGGGEDANAYPERKSDLAEGW"
     gene            1619..2596
                     /locus_tag="AC16CM_00105"
     CDS             1619..2596
                     /locus_tag="AC16CM_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3138257.1"
                     /translation="MEDGRYTNRLAPDAGSNGEANRGSNGGSNGEAKIGPILEQRRKE
                     RNLSLDDVEQATKIRKRYLDGLERDDYDVLPAGVYAQGFLKTYANYLGLDGDELTRQL
                     KSRRKRRPEGRMNYAPGPDNGLEEPLISPAGLQGTGQRRFPTSVVVTLLVAVLVLAVV
                     IGALYFVGRGVETSRPDPNEPPPAENHQAGQNPQKADSEQEPRESGAQEDAAEGNGGV
                     EKAADAGKESGGAGTESSRQGAPPDELEVSIRVSERPSWLLVKADGTVIYAQVVEPGF
                     YSTFRADRRLYIQGGDGGAVSVEINGQDAGALGEPYNVGEKYFTLKNAS"
     gene            2799..3851
                     /gene="recA"
                     /locus_tag="AC16CM_00110"
     CDS             2799..3851
                     /gene="recA"
                     /locus_tag="AC16CM_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3138258.1"
                     /translation="MEDALDKTKAIDAAVSQIERQFGKGSIMRMGDEERPRVASIPTG
                     ALALDLALGVGGVPRGRIVEIFGPESSGKTTLALHIISEAQKAGGLAAFIDAEHAIDP
                     TYAQAIGVDLEDLYFSQPDNGEQALEIADTLVRSGALDVVVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKLSGSLNRSGTTAIFINQLREKIGVMFGSPETTPGGRALKF
                     YSSVRLDIRRIGALKVGTDAVGNQTRVKVVKNKVAPPFKTVEFDVMYGEGISREGSLL
                     DVGIENDVVQKSGAWFAYGDERLGQGRENAKQFLKEHDDVRDRVVNDIYDKLGFNEPA
                     EEVAEEVAEEPASQVF"
     gene            4020..4625
                     /locus_tag="AC16CM_00115"
     CDS             4020..4625
                     /locus_tag="AC16CM_00115"
                     /inference="COORDINATES: protein motif:HMM:NF047066.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR3138259.1"
                     /translation="MTDVRERRGKARVFVDGEYWAELDSGVALERGLRVGAVFNWEEL
                     GRARVAGERPVAMGRALNLLGYRARSEAEIRDRLGRYGYAGETVEAVVGRLRELGYVD
                     DAEFARLKAREKARKYGPRRVSVELRKSGVGEKLAQEVVEEEFAGRSEVGEARSAAAR
                     RYNGRGSDAEARRVYGFLVRRGYSAEVCAQVAREYREPPEA"
     gene            4692..6236
                     /gene="rny"
                     /locus_tag="AC16CM_00120"
     CDS             4692..6236
                     /gene="rny"
                     /locus_tag="AC16CM_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3138260.1"
                     /translation="MSVLLLVLGLVLGVAAGAVLGYYVYRSRVDSRQTQARSDAASIL
                     EDAEREAETKRREADLVAKEAALRLKDDAEAEIRTRRAEIARVEERLDNRDTSLERRE
                     GELNERRRRLTQIEDDLARQREELKERQAEHLKVLEELSGLTRTEAERRLFADLESEL
                     ESRLGQMVRDRTLEAEERADLEARRLISATMERLASDLTSESTVKAVSLPSDDMKGRV
                     IGREGRNIRAFEASTGVDVIIDDTPETVVISSFDPVRREVARVSMGRLVQDGRIHPGR
                     IEQIVAKAKKDVEKEMKSAGRQALYDAKISGSMNNDLIRLLGALKYRTSYGQNVLAHS
                     VEVANLCGMMAQELGANVKISRRAALLHDVGKAIDHEVEGTHALIGGRFAKKSGESDD
                     VVRAISAHHHEVEMETVEDVLVATADAVSAARPGARRETTETYIERLRSLEDIALGHR
                     GVDKAYAIQAGREIRVMVQPSEVDDRIAAKLAYDISKQIETDLEYPGQIKVTVIRESR
                     VSEVAK"
     gene            6312..>6616
                     /locus_tag="AC16CM_00125"
     CDS             6312..>6616
                     /locus_tag="AC16CM_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138261.1"
                     /translation="MEGPYRDLGAGFARLTGVEGARRGPSRIGYVEDALLELLRNARD
                     AGARHVFVASTLARKRYRTLTVIDDGHGIPDSHRDLVLEPGVTTRHLDPVTHPEDLPS
                     "
BASE COUNT         1231 a         2043 c         2319 g         1023 t
ORIGIN      
        1 cctgacccgc agcccgtccg ggacctgcac gaggagttgg tcgagcctag tatcgaaccg
       61 accagggaga tcgaggtggt gctgcccgag cggcggcctg cggaggcgtt cgaccaggag
      121 cgggagctgg gacctggtga gtacacgccg cctgcgttct cgttgcttgg cctcggcacc
      181 ggcgaccccg agcacgacgc ggagggcacc agccgcaggc taactgaggc tctgtcggac
      241 ctcggggtcg aggcccatgt ggtaagggcc gtcgtggggc cgcgggtcac ccgttacgag
      301 atgcgccttg gtagcggggt gaaggtcggc aaggtcagga acctgcagca ggatatcgca
      361 tacgcgctgg cggccacgga ggtcaggatc ctggcgccga ttcccggcaa gagcgccgtt
      421 ggggtcgagg tgccgaacac caggccggcc aaggtcacgc tcggggacgt gttccagtcc
      481 tacccggatg ccaacgactg gacgcttcct gtgggcctgg gcaaggatat ctccgggcgt
      541 gccgtcttct tcgacctctc cgagatgccc cacttgctgg tggcgggtac aacgggaagc
      601 ggcaagagcg tcatgctcaa cgggcttctg acctccctgc tcctaaccac cgacccgcgc
      661 caggtcaaga tggtcctgat cgaccccaag cgtgtcgagc tctcgcagtt cgggcgggtg
      721 cctcacctca taacgcccgt ggtgacggac gtaaagaagg ctgccaacgc gctcacgtgg
      781 ggcgtcgcgg agatggagcg gcgctacgag gtgctggaga ggaacggcgt tcgctctcta
      841 gaagggtaca acaccaaggc cgaggtcccg atgccctacg tcgtcgtggt catagacgag
      901 ctggccgacc tcatgatgca ggccggcgcc aaggtcgagg acgccatcat caggctggcg
      961 cagaaggcgc gggccgtcgg aatacacctc gtggttgcca cccagcggcc aagcgtcgac
     1021 gtcataaccg gcatgatcaa ggccaacgtc ccgagccgca tcgcgttcgc cgtctcgagc
     1081 caggtcgata gccgcgtgat catggacaac cctggcgcgg aggccctgct cggtatgggc
     1141 gacatgctct acaaacccgt ctcggcggcc agaccctcgc gggtgcaggg cgcgtttatc
     1201 tccgaggccg aggtagagcg ggttgtctcg gccacggtgg aggctgcctc cggacgggcc
     1261 gaggccaggt tcgtggagga ggtaacggag ccccgcaacg tcaaagaaga gaacgacgag
     1321 ccggacgacg atctactgcc cgacgcggcg agcttcgtgg tctccacgca gcaggcctcc
     1381 gtgagcgcgg tgcagcggcg cttcagggtc ggctactcgc gggccggcag gatcatcgac
     1441 gccctcgaga gaaagggtgt ggtcggtccc tacgaggggt ccaagagccg ggccgtggtg
     1501 gcgtcgccga tggacctcga gaccctattc ggggggggcg aggatgccaa cgcgtacccc
     1561 gagagaaaga gcgatctggc cgagggctgg tagcttttgt ggtaggctca taatctccat
     1621 ggaagacggg cgatacacca ataggctggc gcccgacgcc ggctccaacg gtgaggccaa
     1681 ccgcggctcc aacggtggct ctaacggcga ggccaagatc gggcctatcc tcgagcaaag
     1741 gcgcaaggag agaaacctct ccctggatga tgtggagcag gcgaccaaga tccgcaaacg
     1801 ctacctcgat ggcctggagc gtgacgacta cgacgtattg cccgccgggg tatacgccca
     1861 gggcttcctc aagacttacg ccaactacct gggcctcgac ggggatgagc tgaccaggca
     1921 actcaagagc cgcaggaaac gacgacccga gggcagaatg aactacgccc ccggcccgga
     1981 caacggcctg gaggaacctc tgatctcccc ggcgggcctg cagggtaccg ggcagcgccg
     2041 cttccccacc tcggtcgtcg tgacccttct ggtcgccgtg ctcgtgctgg ccgtcgttat
     2101 cggggcactt tacttcgtgg gccgcggcgt cgaaacctcg cgcccggacc ccaacgagcc
     2161 tccccccgct gagaaccacc aggcggggca gaacccgcag aaagccgact cggagcaaga
     2221 acccagggaa tccggcgctc aggaggacgc agccgaaggc aatgggggcg tggagaaggc
     2281 cgcagacgcc ggcaaggagt cgggcggcgc gggtaccgaa agctcccgac agggcgcacc
     2341 ccctgacgag ctagaggtct cgatcagggt gtcggagcgt ccctcgtggc tgctcgtcaa
     2401 ggccgacggc acggtaatat acgcgcaggt ggtggagccc ggcttctaca gcaccttccg
     2461 ggccgaccgg aggctataca tccagggcgg tgacggtggg gccgttagcg tggagatcaa
     2521 cggccaggac gcgggtgccc ttggtgagcc atacaacgtc ggcgagaagt acttcaccct
     2581 caagaacgcg agctagcgct catgctcatc gtgagaggag gcattaggtc gcctcgcaca
     2641 gcccgaaacc cgacgccttc tgccaatata tgcgccgctt ctccgagtcc ccggttcccg
     2701 attaccggtc tctcttacgg tattgggagg tacgggtggc tacagtataa tctgacctag
     2761 aagatgccga atatattcgg tgtcggtaca ttcgctttat ggaggatgca ttggacaaga
     2821 caaaggccat agacgccgcc gtctcgcaga tagagcggca gttcgggaag ggctcgatca
     2881 tgcgcatggg ggacgaggag cggcccaggg tcgcctcgat cccgaccggg gcgctggccc
     2941 tcgacctcgc cctcggcgtg ggcggcgtgc cgaggggccg cattgtggag attttcggcc
     3001 ccgaatcgag cggcaagacg accctcgccc tgcacataat ctccgaggcc cagaaggccg
     3061 ggggcctggc ggccttcata gacgccgagc acgccattga cccaacctac gcccaggcga
     3121 tcggcgtgga tctggaggac ctctacttct cccagccaga taacggcgag caggcccttg
     3181 agatagcaga caccctcgtc aggagcgggg ccctggacgt ggtcgtgatc gactccgtcg
     3241 ccgcgctcgt acctagggct gagatcgagg gtgagatggg cgactctcac gtggggctcc
     3301 aggcccgtct gatgagccag gccctccgca agctctcggg ttcgttgaac cgttctggca
     3361 cgacggccat cttcatcaac cagctcaggg agaagatcgg ggtcatgttc ggctctccgg
     3421 agacgacgcc gggcggcagg gcgctgaagt tctactcaag tgtgcgcctc gacatcaggc
     3481 gcatcggggc tctgaaggta ggcaccgatg cggttggtaa ccagaccagg gtcaaggtcg
     3541 tgaagaacaa ggtggcgccg ccgttcaaga cggtggagtt cgacgtgatg tacggcgagg
     3601 ggatctcacg cgagggcagc ctgctggacg tcggcataga gaacgacgtg gtccagaaga
     3661 gcggcgcctg gttcgcctac ggtgacgagc gcctcggtca gggccgtgag aacgccaagc
     3721 agttcctgaa ggagcacgac gacgtgcgcg accgcgtcgt caacgacatc tacgacaaac
     3781 tcggcttcaa cgagcccgcc gaggaggtcg cagaagaagt cgccgaagag cccgcaagcc
     3841 aggtgttcta ggcgagcctc ctgcgcagca gcaggctcgc cgcacgcttc ggcccttcgg
     3901 ggctgcgctt gtcagcacgc ctcctgcggc ggcttgtcgg cacggcgctt tgcgccctgt
     3961 cagctattcg gccggtttga tggaattgag gcttcgtact gagggatgct ttgcctgagg
     4021 tgacggacgt tcgtgagcgc cgggggaagg cgcgggtctt cgttgacggt gagtactggg
     4081 cggagttgga cagtggtgtg gcgctcgagc gcggcctccg ggtgggggcc gtcttcaatt
     4141 gggaggagtt gggacgggcc cgggtagctg gggagcggcc tgtggcgatg gggcgggcgc
     4201 tgaacctgct aggctaccgc gcgcgttctg aggcggagat cagggaccgc ctcgggagat
     4261 acgggtacgc gggggagacc gttgaggcgg tcgtcgggcg tctccgggag ctcgggtacg
     4321 tggacgacgc ggagtttgcg cgactcaagg caagggagaa ggcgcggaag tatgggccgc
     4381 ggagggtttc cgttgagctc aggaagagcg gggtagggga gaagctggcc caggaggtgg
     4441 tcgaggagga gttcgcgggg cgttccgagg ttggggaggc tcgatccgct gccgcacggc
     4501 ggtataatgg gcgtgggtcc gacgcggagg cgcggagggt ctacggcttt ctggtccgtc
     4561 gggggtactc ggcggaggtt tgtgcccagg ttgcccggga gtatcgggag cctccggagg
     4621 cctgagggag agttgggcta gctcgatgcc gggccgccgt attcgcggcg ggtccgctgg
     4681 aaggacaatc tgtgagtgtt ttgttactgg tcctgggact ggtcctcggc gtagccgcgg
     4741 gcgccgtcct gggatactac gtttaccggt cgcgggtgga cagtcgtcag acgcaggccc
     4801 gctccgacgc ggcctccatc ctcgaagacg ccgagcgtga ggccgagacc aaacgccgcg
     4861 aggccgacct cgtggccaag gaggccgcgc tccggctcaa ggacgacgcc gaggccgaga
     4921 tccgcaccag gagggccgag atcgcccgcg tggaggagcg gctggataac cgcgacacgt
     4981 ccctggagcg gcgtgagggt gagctcaacg agcgccgccg acgcctcacg cagatcgaag
     5041 acgacctggc gcgccagcgg gaggagctca aagagcggca ggccgagcac ctcaaggtgt
     5101 tggaggagct ctccggcctt acgcggacgg aggccgagag gaggctcttc gccgatctcg
     5161 agtccgagct cgagagtcgc ctcgggcaga tggtccgtga caggaccctc gaggcagagg
     5221 agcgggccga cctcgaggcg cggcgactca tctccgccac catggaacgc ctggcctcgg
     5281 acctcacctc cgagtcgacg gtcaaggccg tctcgctgcc ctccgatgac atgaagggcc
     5341 gcgtgatcgg tcgtgaggga aggaatatca gggccttcga ggcctcgacc ggggtggacg
     5401 tcatcatcga cgataccccc gagaccgtgg tgatctcgag cttcgatccg gtccgccggg
     5461 aggttgccag ggtctccatg ggacgcctcg tgcaggacgg ccgtatccac cccgggcgca
     5521 tagagcagat agtggccaag gcgaagaagg acgtggagaa ggagatgaag agcgcgggcc
     5581 gccaggcact gtatgacgcc aagatctccg gcagcatgaa caacgacctg atacgcctgc
     5641 tcggcgccct gaagtaccga acctcctacg gacagaacgt gctggcccac tccgtggagg
     5701 tcgcgaacct gtgcggcatg atggcccagg agctcggcgc gaacgtgaag atctcacgcc
     5761 gtgccgccct gctccatgac gtcggcaagg ccatagacca cgaggtcgag ggaacccacg
     5821 ccctgatcgg tggccgcttt gccaagaaga gcggcgagtc cgacgatgtg gtcagggcca
     5881 taagcgccca ccaccacgag gtggagatgg agaccgtaga ggacgtcctg gtcgcgaccg
     5941 cagacgcggt ctcggcggcc cgccccggcg cccgccgtga aaccacagag acctacatag
     6001 agcgccttcg ctccctggag gacatcgccc tcggccacag gggcgtagac aaggcctacg
     6061 ccatccaggc cgggcgtgag atccgcgtca tggtacagcc ctcagaggtc gacgaccgca
     6121 tcgccgccaa gcttgcctac gacatctcca agcagataga gaccgatctg gagtacccgg
     6181 gtcagatcaa ggtaaccgtg atccgcgaga gccgcgtgag cgaggtcgcc aagtaaaatg
     6241 gtgacgtctg cgcgaccacc ctcttggggc ggttcgcgga ccgcccaacc agctgacgcc
     6301 cgaccctgac ggtggagggc ccctacaggg acctcggcgc cggcttcgcc cgccttaccg
     6361 gggtcgaggg cgcccgccgc ggaccctcgc gaataggcta cgtggaggac gccctcctcg
     6421 aacttctgcg caacgcccgt gacgctggtg ccaggcacgt cttcgtagct tcgaccctcg
     6481 ccaggaaacg ctacaggacc ctcacggtga tagacgacgg ccacggcatc cccgactctc
     6541 acagggatct cgtcctcgag ccgggcgtaa cgacccgtca cctcgatccc gtaacccacc
     6601 ccgaggacct accgtc
//
LOCUS       DBNGVD010000005         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_729734, whole genome shotgun sequence.
ACCESSION   DBNGVD010000005 DBNGVD010000000
VERSION     DBNGVD010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_729734"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC16CM_00130"
     CDS             <1..483
                     /locus_tag="AC16CM_00130"
                     /EC_number="4.2.1.136"
                     /inference="COORDINATES: protein motif:HMM:NF013425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0046496 - nicotinamide nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3138262.1"
                     /translation="EVPVLLDADAITNLAGTGVIAERSKPTVVTPHAGELGRMLGSDA
                     REVSARRLDSARTAAGENDCCVLLKGSDTLIVDGQNAAVNPTGDVALATAGTGDVLSG
                     VIGALLSRGMGAYEAARAGAWAHGRAAELWADETGWPGESMVATDLLDHLPRAVGELL
                     "
     gene            492..1268
                     /locus_tag="AC16CM_00135"
     CDS             492..1268
                     /locus_tag="AC16CM_00135"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR3138263.1"
                     /translation="MLFVGDVVGPAGCAAVLDLVPRLREELALDAVVVNAENSAPTGR
                     GITRESGSSLLSVADFLTLGNHAFDAEGYRQFFEEESRVVRPANFGEKDPGLGWGLFQ
                     AGGVTVGVTNVLGRVFVERTKTSPFEAANRAVAALREDGADLVLVDSHAEATSEKLAL
                     AHYLDGRVQAVLGTHTHVPTADLSVLPGGTAYVTDVGMTGCRDSIIGFERDDFLALFL
                     GGWRGISVANHGPVSFNAALVEFDAGRAVGVEAVRREWKP"
     gene            1356..1808
                     /locus_tag="AC16CM_00140"
     CDS             1356..1808
                     /locus_tag="AC16CM_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3138264.1"
                     /translation="MDEIFHKEWPTLGPGDTVESAIKLFAETGISGAPVVDGGKLVGI
                     ITEGDLIFRDADVKAPGFLDILGGMIPLGNWDEYREEAMKSAGVTVDEVMTKEVIMIR
                     PNATLAEASTLMAEKGVKVLPVTEDGDLRGVVTRMDILVLHVLRPQWG"
     regulatory      1900..1980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2030..>3277
                     /locus_tag="AC16CM_00145"
     CDS             2030..>3277
                     /locus_tag="AC16CM_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3138265.1"
                     /translation="MGWWLRSVLQDSLDIVTIVDELGTLMFANPAWERALGYDPAEMV
                     GTMNVLDHVHPEDLPRFSKEWDEIASKGGRKTGPTEYRFRRVDGTWRWLESVGTYLVD
                     DPAVGGVMVNSRDVTERKQTEEALKEAEARYRTLVEQIPIVTYIDRADDSDTSVYTSP
                     QIEELLGYSPEEWRVEKLWAKRLHPEDEARILRADERFEIRGELFDEEYRLLARDGSV
                     VWVREEAVLVEDEAGNPLYWQGVILDITEQKEAVEGLRRSERRFRALTQNSSDLITLM
                     SVTGTIRYQSPSVERILGYGQEETVGDSVFEYVHPGDLSKVEMAFAEGLKDHLRRPSV
                     EYRFRHKDGSWCWIESVGSNMLADPEVGGYVVNSRDVTARRTAEERYRRLAERIPVVP
                     YIEEPGEKGSSTYHVTYMSPRIEE"
BASE COUNT          618 a          966 c         1152 g          541 t
ORIGIN      
        1 gaggttcccg tcctgctcga cgccgatgcc atcaccaacc tcgccggcac cggcgtgatc
       61 gcagaacgaa gcaagcctac ggtcgtgacg cctcacgcgg gggagctggg acgaatgctg
      121 ggatccgacg ccagagaggt atcggcccgc aggctcgatt cggcccgcac ggcggccggg
      181 gagaacgatt gctgcgttct gctcaaaggc tcggacacgc tcatcgtgga tggccagaac
      241 gccgcggtca acccgaccgg tgacgtcgcg ctagccacgg ctgggacggg ggacgttctg
      301 tccggcgtga tcggggcgct tctctcccgt gggatgggtg cttacgaggc cgccagggcc
      361 ggcgcctggg cgcacggtcg ggcggcggag ctctgggcgg atgagacggg ctggccgggg
      421 gagagcatgg tggcaaccga cctgctggat caccttccgc gggccgtggg agagctgctc
      481 tgagcttcag gctgctcttc gtcggggacg ttgtcgggcc cgcgggatgc gccgccgtcc
      541 tggacctcgt tccgcggttg cgtgaagagc tggccctcga cgcggtcgtc gtcaacgccg
      601 agaactcggc gccaacggga cgggggatca cgcgcgagag cggctcctct ctgctctccg
      661 tcgccgactt cctgaccctc ggaaaccacg ctttcgacgc agaaggctac aggcagttct
      721 tcgaagagga gagcagggta gtccgccccg ccaacttcgg agagaaagac cccgggctgg
      781 gatgggggct cttccaggcc ggaggcgtaa cggtgggcgt aaccaacgtg ctggggcgcg
      841 tgttcgtgga gaggacaaag acctcgccct tcgaggccgc gaaccgggcc gttgccgccc
      901 tgcgggaaga cggcgccgac ctcgtgctcg tcgactccca cgccgaggcc acgagcgaga
      961 agctggcgct cgcccactac ctcgacggta gggtgcaggc cgtgcttggg acccacacgc
     1021 acgtgccaac cgccgacctc tccgtcttgc ccggcggaac ggcctacgtg accgacgtgg
     1081 gcatgaccgg ctgtagggac agcataatcg ggttcgagcg agacgacttt ctcgcgctgt
     1141 tcctcggggg ctggcgcggt atctcggttg ccaaccacgg tcccgtgtcg ttcaacgccg
     1201 cgctcgtcga gttcgatgcg ggacgggcgg tcggggtcga ggccgtgcgg agggaatgga
     1261 aaccttgacc gggggttctt ctggccggtc gaatggtgtc aggtcgagac gatcaggggg
     1321 atagagtgga tcagcaggat ataaggaaca tgagggtgga cgagatcttt cacaaggaat
     1381 ggcccacgct tggccccggc gacacggtgg agtctgccat caagctcttc gccgagaccg
     1441 gcatctcggg cgcacccgtc gtcgatggcg gcaaacttgt aggcatcatt acggagggtg
     1501 acctcatctt cagggacgca gacgtgaagg cgcccggctt tctggacatc ctcggcggca
     1561 tgataccgct cggtaattgg gacgagtacc gcgaagaagc aatgaagagc gccggcgtca
     1621 ccgtcgacga ggtcatgacg aaagaggtga tcatgatccg cccgaacgcc acgctggcgg
     1681 aggcctccac cctgatggcg gagaaggggg tcaaggtgct acccgtcacc gaagatggtg
     1741 acctgcgcgg cgttgtcacg cgcatggata tactggtact gcacgtgctc aggccccagt
     1801 ggggatgaaa ggcgggtttg ttcacacgta agaacgctcc aggcgttccg cgaggcgtat
     1861 ttattcgcgg agggggacgc atctaacatt acgtagccgt ctacgaaagg gcaaacccgc
     1921 tgcgaggcgg gggcgcaaag cctacgggcc tctcccaaga gaggtggccg ggctgccgaa
     1981 aggagggcca gaacaaattc gcgccccacg ttcctacggt cccggcgaaa tgggatggtg
     2041 gctacgctca gtcctgcaag actccctgga catcgtcacg atagtagacg aactcggtac
     2101 cctgatgttc gcgaacccag cctgggagcg agcgctgggc tacgaccccg cggagatggt
     2161 cggcaccatg aacgtgctgg accacgtaca tcctgaggat ctgccccgct tctccaaaga
     2221 gtgggacgaa attgcctcga agggcggcag gaagaccgga ccgacagagt atcgtttccg
     2281 acgcgttgat ggcacctggc gctggttgga gagcgttggc acctacctgg tcgacgaccc
     2341 cgcggtggga ggggtgatgg tcaactcgcg ggacgtgacc gagcggaagc agaccgagga
     2401 ggcgctcaaa gaggcggagg cacgttatcg taccctcgtc gagcagatac ctatcgtaac
     2461 ctacatagac agggccgatg attctgatac ttcggtctac accagccccc agatagagga
     2521 gttgctcggc tatagcccgg aagagtggcg ggtcgaaaag ctgtgggcca agcgacttca
     2581 tcccgaggac gaggcgcgta tcctgcgggc cgacgagcgt ttcgagatca ggggcgagct
     2641 gttcgatgag gagtaccggc tgctggccag ggatgggtcg gtggtgtggg tgcgtgagga
     2701 ggccgtgctg gtcgaggatg aggcggggaa tccgctctac tggcagggcg tcatccttga
     2761 catcaccgag cagaaggagg ccgtcgaggg gctcaggcgg agcgagcggc gcttccgggc
     2821 tcttacccag aactcctcgg atctgatcac gctcatgagc gtcacgggca ccatccgcta
     2881 ccagagccct tccgtcgagc gcatcctagg ctacggccag gaggagacgg tgggtgacag
     2941 cgtcttcgag tacgtgcatc ccggggatct cagcaaggtg gagatggcgt tcgccgaggg
     3001 cctcaaggac cacctcaggc gtccatcggt ggagtacagg ttccgccaca aagatggctc
     3061 ctggtgttgg atcgagtcgg tgggatccaa catgctggcc gaccctgagg tgggcggcta
     3121 cgtcgtcaac tcccgcgacg tgacggcccg acggacggcg gaggagaggt accgccggct
     3181 ggccgagcgt atacccgtcg tcccctacat cgaggagccg ggcgaaaagg gttcctccac
     3241 ctaccacgta acgtacatga gccccaggat cgaggag
//
LOCUS       DBNGVD010000006         4320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_911856, whole genome shotgun sequence.
ACCESSION   DBNGVD010000006 DBNGVD010000000
VERSION     DBNGVD010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_911856"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..566)
                     /locus_tag="AC16CM_00150"
     CDS             complement(354..566)
                     /locus_tag="AC16CM_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138266.1"
                     /translation="MKRAIILVTVALAAMLVFAPMGLAQETTMMMGTGSPSPGVPESG
                     GPAILLPAAALLLGSGILTYAILRRR"
     gene            complement(729..813)
                     /locus_tag="AC16CM_00155"
     tRNA            complement(729..813)
                     /locus_tag="AC16CM_00155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(777..779),aa:Ser,seq:tga)
     gene            846..1556
                     /gene="trhA"
                     /locus_tag="AC16CM_00160"
     CDS             846..1556
                     /gene="trhA"
                     /locus_tag="AC16CM_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR3138267.1"
                     /translation="MESISLAECWYGRDVVERSTMKRLREPISGLTHLAGAMLAVVAL
                     GVLLAGAAGEGRLDQFVAFGVFGCSLVALYGASALYHLLPVSPQAVARLRRLDHMAIF
                     VLIAGTYTPICVLALEGGWGVGMLGFVWALAFCGVLLKLFWMGAPRWLTVGLYLGMGW
                     VAVVAIAAILKAVPPGGMAWILGGGLIYSAGAVIYGLKWPNPKPGVFGFHELWHLFVL
                     AGSACHFWAMFRYVSLIA"
     gene            complement(1608..1853)
                     /locus_tag="AC16CM_00165"
     CDS             complement(1608..1853)
                     /locus_tag="AC16CM_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138268.1"
                     /translation="MVVREEEIIQVIPGQFGWFAYGHDEQGKEFLTPVAMWALVESSG
                     NVRHVVGLTAHGLGQVTRVEELPGFVEYRHVSQEEGH"
     gene            complement(1844..2029)
                     /locus_tag="AC16CM_00170"
     CDS             complement(1844..2029)
                     /locus_tag="AC16CM_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138269.1"
                     /translation="MTERDKEREAERKARRQSEEDAERRQEERDRRSDQLRESWRRNH
                     PSEEEEGKAGLKKGRWS"
     gene            complement(2346..2978)
                     /gene="nth"
                     /locus_tag="AC16CM_00175"
     CDS             complement(2346..2978)
                     /gene="nth"
                     /locus_tag="AC16CM_00175"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3138270.1"
                     /translation="MSSSAAQAREVVARLRAEYPDARTELDWNSPLELLVATVLSAQT
                     TDVQVNRVTQTLFTKYRTAKDYASADPSELEEDIRPTGFYRNKARSLRNMAAALVEDH
                     GGEVPGSMQELVALPGVGRKTANVVLGNAFGVDEGIVVDTHVRRVSGRLGLTEKEDPV
                     KIERDLMQVVPGEDWTVFSHLLILHGRRTCKARKPDCPNCTLNDICPSAQ"
     gene            3047..>4320
                     /locus_tag="AC16CM_00180"
     CDS             3047..>4320
                     /locus_tag="AC16CM_00180"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3138271.1"
                     /translation="MAEVTFDYQNMMEVEGGLQSGELDEIQPRLREAADELLGNPPGF
                     MRLPKTKEYAEASARVAEEVRGSGATDFIHVGIGGSALGPMAVQKALSHPYYNALPDG
                     ERPGPRVHFAENTDPATLSAILDLADPQGTHVNVVTKSGSTAETMANFLVIRGALIDA
                     LGDSGFQERAIATTDPEEGFLKQIADREGMKVLPIPADVGGRFSVLTPVGLLPAAVAG
                     LDIDAMLAGAAQCVDEVTEQGAEHPAVVGAAMHYLMDTARGRNVRVMMPYADALERLA
                     AWFVQLWAESLGKDGKGSTPHGAVGTTDQHSQVQLYMEGPEDKVIEIVEVEDHPKDLH
                     IPEAYDDLEGVGYLGGHSMAELLNVECDATRRALTEAGHPNSTIKLSATSAENLGYVF
                     QALEVQTAIAGSLYGVNAFNQPGVEAGKEITY"
BASE COUNT          728 a         1433 c         1347 g          812 t
ORIGIN      
        1 ggtgccgccg aagaggatct tggcgccacg gatcaacccg acgtcgccgc gcgacacgag
       61 tgcgagcaat gccaggcaag cgatgacccc accgtacagg atcacgtcgg gtagcctgtt
      121 tcccgacctg ctcatccgtc cagcctgccc cgatccgccc ttcgcgctag cttgcctcga
      181 atcgtgcggg gagatggccg agcccaaaag gaacggtatc ccgcagcccc ccggaaacgc
      241 ttatggccgc cggtttcccg acggccacga aaaagagccg ggggggtgca ggctcccccc
      301 ggctccggaa ggaccggcct acaaagggac cggcccgcag aatctcgagt ttactacctg
      361 cgcctcaaga tcgcgtaggt cagaatgccc gacccgagca gcagggcggc agccggcaga
      421 aggatggcag gaccaccgct ctccgggaca cccggcgatg gcgacccagt ccccatcatc
      481 atagtggtct cctgcgccag acccatcggc gcgaagacca acatggccgc aagcgcgacc
      541 gtaaccagaa taattgccct cttcatccag tcctcctttt gtttactctg tctccaagtg
      601 gcctgcactt gtctcctcct accgagaagt acccacagtg tacagaaccg aaacgtaaaa
      661 gtctccatta tcttaatgat ttttaacaaa tcggttacgt gggcgtttca tgggtacatc
      721 ccagtgggcg ggggcagaag gattcgaacc ttcgggcgtt ttacggcctc tggttttcaa
      781 gaccagcgca ttcgtccgct ctgccatgcc ccctcaccgc gtctggttgg attatacgat
      841 gtctggtgga gtctatctct ctggcggaat gctggtatgg tcgggacgtg gtcgagcgga
      901 gcacgatgaa gaggttgcgg gagcccataa gcggtctcac ccatctggct ggggccatgc
      961 ttgcggtggt cgcgctcggc gtcttgctgg ccggggcggc gggtgagggg aggttggacc
     1021 agttcgtggc cttcggcgtc ttcggttgca gcctcgtggc gttgtacggg gcgagcgcgc
     1081 tctatcatct gttgcccgtc tcgccgcagg ccgtagcgag gttgaggagg ttggatcaca
     1141 tggccatctt cgtcctcata gccgggacct acacgcccat ctgcgtgctc gcgctggagg
     1201 gtggctgggg ggtggggatg ctcggcttcg tatgggctct agccttctgc ggcgtcttgc
     1261 tcaagctctt ctggatgggc gctccaaggt ggctcaccgt aggcctctac ctgggcatgg
     1321 gctgggtcgc cgtggtagcc atcgcggcca tcctgaaggc cgtgccgccg ggcgggatgg
     1381 cctggatcct cggcggcggc ctcatctaca gcgcgggggc cgtgatctac ggcctcaagt
     1441 ggcccaaccc gaagccgggg gtgtttggtt tccacgagct gtggcacctc ttcgtcctgg
     1501 cgggaagcgc ctgccatttc tgggcgatgt tccggtatgt atcgttgatc gcgtgatcta
     1561 ggttgcccgg caccaggcga agtgcttcgc ggggtgcttc actggcgtca atgcccttct
     1621 tcctgcgaga cgtggcggta ctctacgaac ccaggcagtt cttcgactcg cgtcacctgc
     1681 cccaacccgt gtgcggtcag ccccaccacg tggcgcacgt tgccgctcga ctccaccagc
     1741 gcccacatgg ccaccggcgt gaggaactcc ttgccctgct catcgtggcc ataggcgaac
     1801 cagccgaatt gaccgggtat cacctggatt atctcctctt ccctcacgac caacgtccct
     1861 tcttcagccc ggccttcccc tcttcctcct cgctcgggtg attgcggcgc caggattccc
     1921 tgagctggtc ggagcgccgg tcccgctctt cttgccgtcg ttcggcatct tcctcggatt
     1981 gccttctcgc cttccgttcg gcctcacgtt ctttgtcacg ctctgtcatg cttctccttc
     2041 tccaatccgc agcgcttcgt ctgtgaccag ccctgcaatg tgccctgtgg gctttctgta
     2101 cccgatctcg cctctcgtca ccacacgccc catcctccct tcgttgtgta ccgtccattc
     2161 catctcgccc cgcaaaagcg gggccgccga tagatgtccg gcaggtgctc gaacctctgc
     2221 cagatgtgca cacgacttac ggtacagaac gccacgccgt tcgaggaatg cagctcgggt
     2281 tctggccggc ttcgggcgcc cggcaagccc tcaagggagc agcacgcgca aaccagcgtt
     2341 tcgtcctact gcgccgaggg gcagatatcg ttgagggtgc agttgggaca atcgggtttg
     2401 cgggccttgc aggttcggcg gccgtgcagg atcaggaggt gggagaagac ggtccagtcc
     2461 tcaccaggca cgacctgcat caggtccctc tcgatcttca ccgggtcctc tttctccgtc
     2521 aggccgagcc ttccggagac gcggcggacg tgggtatcca cgactatgcc ctcgtccacg
     2581 ccgaaagcgt tgccaaggac cacgttcgcc gtcttgcggc cgacgccggg cagcgccacg
     2641 agctcttgca tggagcctgg cacctctccc ccatggtctt cgacgagggc ggcggccatg
     2701 ttccgaagcg agcgggcctt gttgcggtag aagcccgtgg gacggatatc ctcctccagc
     2761 tcggaggggt cagcgctggc gtagtccttg gccgtacggt acttcgtgaa cagcgtctgc
     2821 gtcacgcggt tcacctgcac gtctgtagtc tgggcggaga gcacggtggc caccagcagc
     2881 tccagcgggc tattccagtc cagctcggta cgcgcgtcgg ggtactccgc cctgaggcgg
     2941 gcaaccactt ccctggcctg ggctgccgaa gatgacatga tggcccggga ttataccctg
     3001 ttctaaacta cgcccgtcaa tgaatgctac agagaggagc cagcgcatgg ctgaggtcac
     3061 ctttgactac cagaacatga tggaggtgga gggcgggctg cagagcgggg agctagatga
     3121 gatacagccg cgcttacgtg aggccgctga cgaactcctc gggaacccgc cggggttcat
     3181 gcggctgcca aagacaaaag agtacgccga ggcttcggcc cgtgtcgccg aggaagtcag
     3241 aggctcgggg gccacggact tcatccacgt cggcatcggg ggatccgcgc tcggccccat
     3301 ggccgtgcag aaggcgctga gccaccccta ctacaacgcc ctccccgacg gggagcgtcc
     3361 cggcccgcgc gtgcatttcg cggagaacac cgatcccgcc accctctcgg ccatcctcga
     3421 cctcgccgac ccccagggga cccacgtcaa cgtggtcacc aagtccggct cgacggccga
     3481 gaccatggcc aacttcctcg tgatccgggg cgcgctcatc gacgcgcttg gagactccgg
     3541 gtttcaggaa cgtgccatcg ccactacaga ccccgaggag gggttcctca agcagatagc
     3601 cgaccgcgag ggcatgaagg tcctgccaat acccgccgac gttggcgggc gtttctcggt
     3661 gctcacgccg gtcggtctgc tgccggctgc cgtggccggg ctcgacatcg acgcgatgct
     3721 ggcaggcgcg gcccagtgcg tcgacgaggt gacagagcag ggggccgagc acccagcggt
     3781 cgtcggggcg gccatgcact acctcatgga caccgcgcgc gggcgtaacg tcagggtcat
     3841 gatgccttac gccgacgcgc ttgagcgtct cgcggcctgg ttcgtgcagc tctgggccga
     3901 gtctctcggc aaggacggca agggttccac gccgcacggc gcggtcggca ccacggacca
     3961 gcactcgcag gtccagctct acatggaggg acccgaggac aaggtgatcg agatcgtcga
     4021 ggtcgaggac cacccgaagg atctacacat accggaggcc tacgacgacc tcgaaggcgt
     4081 cggctacctc ggggggcact ccatggccga gctcctcaac gtcgagtgcg acgccacgcg
     4141 gcgggccctc accgaggccg gccaccccaa ctcgaccata aagctcagcg cgaccagcgc
     4201 ggagaacctc ggctacgtgt tccaggccct cgaggtccag accgccatag ccggctccct
     4261 ctacggcgtc aacgccttca accagcccgg cgtcgaggct gggaaggaga tcacgtacaa
//
LOCUS       DBNGVD010000007         5519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3644836, whole genome shotgun sequence.
ACCESSION   DBNGVD010000007 DBNGVD010000000
VERSION     DBNGVD010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3644836"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..3660
                     /locus_tag="AC16CM_00185"
     CDS             85..3660
                     /locus_tag="AC16CM_00185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3138272.1"
                     /translation="MLEGRERAVVEIPVTGMTCASCVRRVEKALGSVEGIEEATVNLA
                     TEKARVSYDPGVADGRSMRSAVEKAGYGVREMPAPTPASKPSTASGGGNEVLLPVAGM
                     TCASCVSSVEKALQKVPGVESATVNLATEKAMVSLAPSGGADIVDLRAAVEKAGYGVG
                     EVEGPVGAPSPGRVMPGFGAEADPREREREAELEDLRNKWVVSLAIGLLIMLEMYLPF
                     GPEEALLAPLLLIQATIVQFWAGSTFYRTAWASARHGSTNMSTLVAVGTSVAYGYSAF
                     VTLWPDLSAAWGFPFYLYFEVAAIVIALVLLGRWMEARAKKRTGAAIKALMGLQAKTA
                     RVIRDGTEADIPVESVEVGDLVRVRPGEKVPVDGVVVEGRSALDESMLTGESLPAEKS
                     VGDAVIGATLNKTGGFVMRAEKVGSDTALAQIVRMVEEAQGSKAPMQRTVDTISSYFV
                     PAVLVLAAITFLIWLVFGPSLIFALTALISVLIIACPCALGLATPTAIMVGTGKAAEN
                     GILVKGGEALEMTRKVDAIVLDKTGTLTKGKPTVTGVIPADGITEEELLRLVATAEVG
                     SEHPLAEAIVVRAEERGLELPTAERFESVTGKGVRATVDGREVAIGNRTFMDEIGAHP
                     NGLSERAAVLARGGATPMYVSVDGRSAGIVTVADTLKPESREAVEELRALGLGVWMLT
                     GDNRATAEAIADQVGIDPEHVLAEVLPEEKAARVAELRASGKTVAMVGDGINDAPALA
                     GSDLGIAIGTGTDVAMAASDITLIGGDLRNIVAGIALSRKTTSKIKEGLFWAFAYNVA
                     LIPVAAGALYPSFGILLSPVLAAAAMAMSSVSVVTNALRLRSFERPESAEEILHPPLR
                     ARIGEVAYLGTIAVVALGVGAAAVYLAPAGHGDMPAAGQGSGAPNAADANNGAHAGTN
                     DAAGHGGARAEHGSGASDVVSPERAGARVEFAANPAIPRPGGPVELSYTIRDAGSGEA
                     ITDLPLDHERPMHTIVVGSDLEHFAHIHPRAQGEGSYRVETALPDAGTYNLYTEFVRG
                     GDKVLDRRVLAVGNGGGGVANLSPDLSPKTVNGTTVAIDVPENIQAGEPIHLDFELSQ
                     DGGKPVSDLSPYLGAAAHVAIVSEDGEDFAHGHGEVKDTEEAARADDHTGEEHQEGGH
                     DVPSSFGPSVRADHTFEDPGLYKIWAQFERDGRVLTVPFLVEVGR"
     gene            3750..4124
                     /locus_tag="AC16CM_00190"
     CDS             3750..4124
                     /locus_tag="AC16CM_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR3138273.1"
                     /translation="MSTIPANGEQADVSPDPGGEETMTEQTRQAHGYIKEGDKEKLRN
                     RLRRIEGQVRGVQRMIEKEAYCVDILTQISSYIAASEKVASLVLRDHMDHCVREAVED
                     GSKADEKIEELAEAVERFVKLD"
     gene            4215..4427
                     /locus_tag="AC16CM_00195"
     CDS             4215..4427
                     /locus_tag="AC16CM_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3138274.1"
                     /translation="MIKTEVLMVPDMTCGHCEASVQGALAALPGVEGVKADSVTGRVE
                     VSYEGAKTDRVQFGSAVEEAGYTLEA"
     gene            complement(4552..5286)
                     /locus_tag="AC16CM_00200"
     CDS             complement(4552..5286)
                     /locus_tag="AC16CM_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015057.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR3138275.1"
                     /translation="MNAEEFLGRLKRDVIGHPALSHPFLERFAEGEADAEGIRTFAIQ
                     YYRHVRVSRLYLAALISQCREDEGLQLALAEVLFDEYGHLDPNETHPALYRRFLTALG
                     IGEEEWEEPPTMPEIEMYIATHFALSGHPDFRIGLGALGPASEWPVPPIYARLSDGLR
                     RAAGLPEAALEIFTSHVTMDVTHARIMMEAIAPYVEDEGGQQKVREGAIRSLDARSVM
                     LDGLYRAVYREPVPVFDAPQVRLTGG"
BASE COUNT          973 a         1791 c         1918 g          837 t
ORIGIN      
        1 cccgcaggcg aacaggacct tgacacttca gaatacgtga tatagggtag gggggtatag
       61 tacaccacag gaaagagagg tgcggtgctc gaaggacggg agcgggcggt agtcgagata
      121 ccggttacgg gcatgacctg cgcttcttgt gtgcggcgcg tcgagaaggc tcttggcagt
      181 gtcgagggca tcgaggaggc gaccgtcaac ctggcgacgg agaaggccag ggtttcgtat
      241 gatccggggg tcgccgacgg gcggtcgatg aggtcggctg tggagaaggc cggttacggg
      301 gtgcgggaga tgcccgctcc tactccggcg tcaaagcctt cgaccgcatc cggtgggggc
      361 aacgaggtgc ttctgccggt tgcggggatg acgtgcgcct cctgcgtgag cagcgttgag
      421 aaggcgctcc agaaggtgcc tggtgtagag tccgcgaccg ttaatctggc gacggagaag
      481 gcgatggtgt cgctggcccc gtcgggcggg gcggatatcg tcgacctgcg cgccgcggtc
      541 gagaaggccg gatacggggt cggcgaggta gaggggccgg ttggggcgcc ttcacccggg
      601 cgggtgatgc ccgggtttgg cgcggaggcc gacccgaggg agcgcgagcg tgaggcggag
      661 ctcgaggacc tcaggaacaa gtgggtggtc agcctggcaa tcggcctcct gatcatgctc
      721 gagatgtacc tgcccttcgg gccggaagag gcgctgctcg cgccgctctt gctgatccag
      781 gccacgatag tccagttctg ggcgggtagc accttctaca ggacggcgtg ggcctccgcc
      841 aggcacggct ccacgaacat gagcaccctg gtagccgtag gcaccagcgt cgcctacggc
      901 tacagcgcgt tcgtgacgct gtggccggac ctcagcgcgg cgtggggctt cccgttctac
      961 ctgtacttcg aggtggcggc catcgtcatc gcgctcgtcc tgcttggccg ctggatggag
     1021 gcgcgggcca agaagcggac cggcgccgcg ataaaggcct tgatggggct gcaagcgaag
     1081 acggcgcgcg tgatccggga cgggactgag gccgacattc cggtcgagag cgtcgaggtc
     1141 ggagacctcg tcagggtccg tcctggcgag aaggtcccgg ttgacggcgt cgtggtcgag
     1201 gggcgctcgg cgctcgacga gagcatgctc acgggcgagt cgttgccggc ggagaagtcg
     1261 gtcggggatg cggtcattgg cgccaccctc aacaagaccg gtgggttcgt catgcgtgca
     1321 gagaaggtcg gctccgatac ggcgctcgcc cagatcgtgc gcatggtcga ggaggcccag
     1381 ggatcgaagg ccccgatgca gcgaacggtc gacaccatct catcttattt cgtcccggcg
     1441 gtgctagtgc tggccgccat caccttcctc atctggctcg tcttcggccc gtccctgatc
     1501 ttcgcgctga ccgccctgat ctcggtcctg atcatcgcct gcccatgcgc cctgggcctc
     1561 gcgacgccga cggcgatcat ggtcgggacc ggcaaggccg ccgagaatgg gatcctcgtc
     1621 aagggcggcg aggcgctcga gatgacgcgc aaggtggacg ccatcgtcct ggacaagaca
     1681 ggcacgctca cgaaggggaa gcccacggtc acgggcgtga tccctgccga cggtattacc
     1741 gaagaggagc tgttgcgcct ggtcgccacc gccgaggttg gctccgagca cccgctcgcc
     1801 gaggccatcg tcgtgcgggc cgaggaacgg ggcctggaac ttccgacggc cgaaaggttc
     1861 gagtccgtca ccggcaaggg cgtcagggcc acggttgacg ggcgggaggt cgccatcggc
     1921 aaccggacct ttatggacga gatcggcgcg cacccgaacg ggctttcgga aagggccgcg
     1981 gtgctggcgc gcgggggcgc cacgccgatg tacgtatccg tcgacggacg gagcgccggc
     2041 atcgtaaccg tagccgacac cttgaagccg gaatcgcggg aggcggtcga agagctcagg
     2101 gctctgggcc tcggggtctg gatgctcacc ggcgacaacc gcgccaccgc cgaagccata
     2161 gcggaccagg tcggcataga ccccgagcac gtgctggccg aggtgctgcc cgaggagaag
     2221 gcggccaggg tcgccgagct gcgggcgtcg ggcaagaccg tggcgatggt cggggacggc
     2281 ataaatgacg cccccgcgct cgccgggtcg gatctcggaa tagccatagg cacgggcacc
     2341 gacgtggcca tggccgcctc ggacatcacc ctgatcggcg gggacctcag gaacatcgtc
     2401 gccggcatag ccctgtcgcg caaaaccacc agcaagataa aggagggcct gttctgggcc
     2461 ttcgcctaca acgtcgccct gatcccggtc gccgcgggtg cgctctaccc gtccttcggc
     2521 atcctcctga gcccggtcct ggcggcggcg gctatggcga tgagcagcgt gagcgtggtg
     2581 acaaacgccc tcaggctccg ctccttcgag cggccggaga gcgcggagga gatactgcac
     2641 ccgcccctgc gcgcccgcat aggcgaggtc gcctacctcg gcaccatcgc cgtggtcgcc
     2701 ctcggcgtcg gggccgccgc cgtctacctc gcccccgcgg gccacgggga tatgccagcc
     2761 gcgggccagg ggtccggcgc accgaacgcc gcagacgcga acaacggggc gcacgcgggt
     2821 acgaatgacg ccgccggaca cggtggcgcc cgcgcggagc acggttccgg cgcttcagac
     2881 gtggtatcgc ccgaaagggc cggcgcgcgc gtcgaattcg ctgcgaaccc cgccatcccc
     2941 aggccagggg gtcccgtcga gctctcctac accatccgcg acgccgggag cggcgaggcg
     3001 ataacggacc tccccctcga ccacgaacgc ccaatgcaca cgatcgtcgt tggctccgat
     3061 ctagagcact tcgcccacat ccacccgaga gcccagggtg aaggctccta ccgcgtcgag
     3121 acggcgctgc ccgatgccgg gacctacaat ctctacaccg agttcgtccg tggcggcgat
     3181 aaggtcctcg acaggcgcgt gctcgccgta ggaaacggag gtggcggcgt tgccaacctc
     3241 tcccccgacc tgtctcccaa gacggtaaac ggaaccaccg tcgccatcga cgtgcccgag
     3301 aacatccagg ccggcgagcc catccatctc gacttcgagc tctcgcagga cggtgggaag
     3361 cccgtaagcg acctctcacc ctacctcggg gcggcggccc acgtcgccat cgtctcggag
     3421 gacggcgagg acttcgccca cggccacggt gaggtgaagg acacagagga ggccgccagg
     3481 gccgacgacc acaccggcga ggaacaccag gaggggggcc acgatgtccc ttcatcgttc
     3541 gggccaagcg tccgcgccga ccacactttc gaagatcccg gcctgtacaa gatctgggcc
     3601 cagttcgaac gtgacggccg cgtcctgacc gtccccttcc tcgtcgaggt cggacgatga
     3661 gccgccgcga cggcagtccc gaggcccgcc ggagaccgcg aaccggggac gttcgtgccg
     3721 acggtcttcg attgataccc ccgccgggta tgagtacaat cccggcaaac ggggagcagg
     3781 ccgatgtctc ccccgaccca ggaggcgagg agacgatgac ggagcagacg cggcaggcgc
     3841 acggctacat caaggaaggg gacaaggaga aactcaggaa ccgcctcagg cggatcgagg
     3901 ggcaggtgcg gggcgtgcag cgcatgatcg agaaggaagc ttactgcgtg gacatactca
     3961 cgcagatcag ctcctacatc gccgcctccg agaaggtcgc atcgctggtc ctgcgcgacc
     4021 acatggatca ctgcgtcagg gaggctgtcg aggacggctc caaggcggac gagaagatag
     4081 aggagttggc cgaggccgtc gagaggttcg taaagctaga ctgagccggg tctgcctcgg
     4141 ggtttccagc cgtccactcc cgctgacaag atgaaaacgc gcagaaaacc cgaaccccag
     4201 gagaggagaa agtgatgata aagacagagg tgctgatggt accggacatg acgtgcggcc
     4261 actgcgaggc gagcgtgcag ggggccctgg cggcgcttcc cggcgtcgag ggggtaaagg
     4321 cggattccgt cacaggtagg gtcgaggtgt cctatgaggg agcaaagacg gaccgcgtgc
     4381 agttcgggtc cgccgtcgag gaggccggct acaccctcga ggcgtagggt ccgcgggccc
     4441 acgcggcctt agggccgcca gcctctgtaa cgccgtctac ctcgccaccg gggcggtcgc
     4501 agagctgcgc cccctgaagc ccaggtcttc cccgtactac ccgtccgggc ctcaaccgcc
     4561 ggtgagccgg acctgagggg cgtcgaagac cgggacgggc tccctgtaga cggccctgta
     4621 gaggccgtcg agcatcacgg agcgggcatc gagggagcgt atggccccct cgcggacctt
     4681 ctgctgtcca ccctcgtcct ccacgtacgg ggctatggcc tccatcatta tccgggcgtg
     4741 ggtcacgtcc atggtgacgt ggctggtgaa gatttccaga gccgcctcgg ggagcccggc
     4801 ggccctcctg agcccgtcgg agagcctggc gtagatcggg ggcacgggcc actcgctggc
     4861 cggcccaagg gcgccgagcc cgatcctgaa atccgggtgc cctgacagag caaagtgcgt
     4921 ggcgatgtac atctcgatct cgggcatcgt aggcggctct tcccactcct cctcgccgat
     4981 accaagtgcc gtaaggaatc tccggtagag ggcagggtgc gtctcattcg ggtccaggtg
     5041 gccgtactcg tcgaagagta cctcggcgag ggctagctgc agcccctcgt cttcgcggca
     5101 ctgcgagatg agcgccgcaa ggtacaaccg gctcaccctg acgtgcctgt agtactggat
     5161 cgcgaacgtc ctgatcccct ccgcgtccgc ctccccctcc gcaaaccgct ccaggaaagg
     5221 atgcgagagc gcgggatgcc cgatcacgtc ccgcttcaag cgtcccagaa actcctcagc
     5281 attcacccgg ctctccttcc agcacgctcc ggctgcgcct ccgcgtgcgc acgccgccct
     5341 gcgggcggct tgtcagcacg gcgcttcgcg cctccttagc acgcttcgcc cggctccgcc
     5401 aggctcgctt tcagctaata gtgccgcagc atacccttct gcatatagtg taccgctacg
     5461 gggggaaggt agatacccgg ccaccgttac aaatacagac gactggccga aagcgaggc
//
LOCUS       DBNGVD010000008         3645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1458602, whole genome shotgun sequence.
ACCESSION   DBNGVD010000008 DBNGVD010000000
VERSION     DBNGVD010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1458602"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="AC16CM_00205"
     CDS             complement(<1..1005)
                     /locus_tag="AC16CM_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3138276.1"
                     /translation="MRKWLLILALAGCLIVVGCSGGSEEAGRGDGGEDDGGDAESTKP
                     LIGSDLGHQAFQSSTRLVVPDGVGTTFDRVGRVLAGVAEGTLGVRMFVNQRPGEEGFV
                     AWRDVAAEEPEGHQLAYVTEGLLDTGEIGPEDFEMVAQTDRGFAVLVAKGDPEIETLQ
                     GDDLDDFGDFVKGAREDPGFMEVADMGPETVYRAGTLELEDELGVDLSPKSPAHKSPV
                     EAIYDGDVETALVPLDWNLYGDLLAGELTPLAVLAGERTPELPGVPTAKELGYKVTVP
                     VFGGVVAPAGTPSDVVQELGRAFVGASSSRAFQRALLGTGREPEQKGPEKFRAYIDRQ
                     T"
     gene            1161..1784
                     /locus_tag="AC16CM_00210"
     CDS             1161..1784
                     /locus_tag="AC16CM_00210"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HIR3138277.1"
                     /translation="MNPVANNTFFDSEPGGRVAAPGSLALVQAFVNTVAQEGEHRWEA
                     FSGPTSLRDWLGQNGLLPEDEAVGESDVVWVKEVRRGIRSLLGANNGGEVDPGALITL
                     NRAAERAALLVRFSPDGRAAIGAGADGVEGAIGKVLESVVAGMEDGTWGRLKTCANVG
                     CGWAFYDRSRNRSGKWCSMEVCGNRSKTRTYRRRASLVREGQDHRGR"
     gene            complement(1869..2669)
                     /locus_tag="AC16CM_00215"
     CDS             complement(1869..2669)
                     /locus_tag="AC16CM_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138278.1"
                     /translation="MAGSPRGRHGRVLRSSERVYRSLLRAYPRGLRDEYGEEMTLCFR
                     DLCTEELENRGRRGLMALWAHTLTELIYTALKERRSTLARNTYQSVLGVAIVTALILL
                     IPLLTAPAWTPDDFVRAGALLFGTGLAFVLVVRGGGNFAYRSAVVVALAAALFLVWVN
                     LSVGIIGEPNEGANLMYMGVLAVGIGGVFVARLRPQEMARALLATSLAQALVAVIALT
                     FGLGAGSPPGVLGVLILNGFFVLLFAGSALLFRYAGREQTPAGASPGV"
     gene            complement(2669..3055)
                     /locus_tag="AC16CM_00220"
     CDS             complement(2669..3055)
                     /locus_tag="AC16CM_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3138279.1"
                     /translation="MGDENVMGERSGGPEEFLPLTPASLNVLLALADGDRHGYGIMLE
                     VRERTGGSVRLGPGTLYGAIKRLKEGGVIEESGGDAGAEDERRRYYRLTGFGGEVLAA
                     EVERLDGLVRAARRKGAFPDPRPAGT"
     gene            complement(3410..>3645)
                     /locus_tag="AC16CM_00225"
     CDS             complement(3410..>3645)
                     /locus_tag="AC16CM_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3138280.1"
                     /translation="PGFGGQSFLPEAAEKIRRLKEMTGLPIAVDGGITVETAPVAAEA
                     GANALVAGSAVFKGDPATEMRRIIEAGQGYAEK"
BASE COUNT          625 a         1260 c         1111 g          649 t
ORIGIN      
        1 ggtctgtctg tcgatgtagg cccggaattt ctctggtcct ttttgctccg gttctcttcc
       61 agttccaagt agcgccctct ggaacgcgcg cgaggaggaa gcgcccacga acgcccgtcc
      121 cagttcctgg accacgtctg atggtgtgcc ggccggcgcg accacccccc cgaagaccgg
      181 caccgttacc ttgtacccga gttctttcgc ggtggggact ccgggaagct cgggcgtacg
      241 ctccccggcg aggaccgcta acggggtgag ctctccggcg agcaggtcac cgtacaggtt
      301 ccagtcaagc ggtaccaggg ccgtttccac gtcgccgtcg tagatggctt cgacgggcga
      361 cttgtgtgcc ggcgacttcg gcgagaggtc tacgccaagc tcgtcctcca gttccagggt
      421 cccggcccgg tatacggtct ccggccccat gtccgccacc tccatgaacc cggggtcctc
      481 ccgcgccccc ttcacgaagt ccccgaagtc gtcgagatcg tccccctgga gtgtctctat
      541 ctcgggatcc cccttcgcca cgagcacggc gaagcccctg tccgtctggg cgaccatctc
      601 gaagtcctca ggcccaatct ccccggtatc tagcagcccc tcggtcacgt aggcgagctg
      661 gtgcccctcc ggctcctctg ccgcgacgtc gcgccaggcg acgaaccctt cctccccagg
      721 acgctggttc acgaacatgc gtacgcccag cgttccctcg gccaccccgg ccagcacacg
      781 cccgacgcgg tcgaaggtgg tgccgacccc gtccggaacg acgagccgcg tggaagactg
      841 gaacgcctga tgcccgagat cactcccgat cagtggtttc gtgctctcgg cgtccccgcc
      901 atcgtcctcc cccccatcgc ccctgccagc ctcctcgctt ccgcccgagc acccgaccac
      961 gatcaaacat cccgccaggg ccaggatcag caaccacttc cgcatgccct acctacctct
     1021 tctcggggga cagcaacgcc tggatgggcc cctgctagat gagttgcaac cgccctttac
     1081 tttacaccgg tgtttgcaca ccggtcaaat atgttggatg gtgtttcacg cgcgtcctgg
     1141 ccggatatat actggggatc gtgaaccccg tggcaaacaa caccttcttc gattcggagc
     1201 ctggggggcg cgtggcggct ccgggttcgc tggcgttggt gcaggccttc gtgaacaccg
     1261 ttgcgcagga gggcgagcac aggtgggagg ctttctccgg gcctacctcg cttcgtgact
     1321 ggctggggca gaacggtctg ctccccgaag acgaggctgt cggggagtcg gacgtcgtat
     1381 gggtgaagga ggtcaggagg gggatccgtt cgctgctcgg ggccaacaac ggtggtgagg
     1441 tcgacccagg ggccctgatc acgctcaaca gggctgccga gcgggccgct ctgctagtac
     1501 gcttcagtcc ggatgggcgt gcggccatcg gggccggcgc ggacggcgta gagggcgcta
     1561 tcggcaaggt gcttgagagc gttgtcgccg ggatggagga tggtacatgg gggcgcctga
     1621 agacctgcgc caacgttggt tgtggctggg cgttctacga tcgctccagg aaccgctctg
     1681 gcaagtggtg ctcgatggag gtgtgcggca acaggtccaa gacccgcacg taccgccggc
     1741 gtgcctcctt ggtacgcgaa ggtcaggacc atcgtggtcg gtgaaggagt tgcgccctgt
     1801 accttcgcgc acgggtgctt ctcctcatgg tctgtcctgc gggagtcggg gcgtctggag
     1861 gtagcgggtc agacccccgg cgaggccccg gcgggggttt gctcccgccc ggcgtagcgg
     1921 aacaacagcg ccgacccggc aaacagcaag acgaagaacc cgttcaggat caggacccct
     1981 agaacgcctg gcgggctccc cgcccctagc ccgaaagtca gggcgataac ggcgaccagt
     2041 gcctgcgcga gcgacgtcgc caaaagagca cgagccatct cttgcggccg caggcgcgcg
     2101 acgaagacac cgccgatccc gacggccagc acccccatgt acatcagatt ggcgccctcg
     2161 ttcggctccc caatgatgcc gacggaaagg ttcacccaga cgagaaaaag cgccgccgcg
     2221 agcgcgacga cgacggcgga ccggtaggcg aagttgccgc ctccccttac caccagcacg
     2281 aacgcgagac ctgtgccgaa tagcagggcg ccggctcgga cgaaatcgtc cggcgtccac
     2341 gccggcgccg tcaacagggg gatcaggagt ataagcgccg ttacgatcgc gacgccgagg
     2401 acggattggt aggtgtttct tgccaacgtg gatctcctct cttttaacgc ggtgtagatc
     2461 aactccgtca gggtgtgggc ccataatgcc atcagacccc ggcggccacg gttctccaac
     2521 tcctcggtac acaaatcccg aaaacagagc gtcatctcct ccccgtactc gtcccggagc
     2581 ccgcgaggat aggcccgcag gagagaccgg tagacccgct ccgacgaccg taggacgcgt
     2641 ccgtgccgtc ctctggggga tcccgccatc acgtccccgc cggacgtgga tcgggaaacg
     2701 cgcctttccg gcgcgcggca cgtaccaggc cgtcgagcct ttcgacctcc gccgccagga
     2761 cctctcctcc gaagccggtg agacggtagt aacggcgccg ctcgtcttcc gcccccgcgt
     2821 cacccccgga ctcctcgatc acaccgccct ccttcaaacg cttgatcgcc ccgtagaggg
     2881 tcccgggacc cagccgcacg ctaccacctg tcctctccct cacctcgagc atgatcccgt
     2941 acccgtgacg gtccccatca gccagcgcca gaagcacgtt cagcgaggca ggcgtcaagg
     3001 gtaaaaactc ctccggccca ccagaccgct cccccattac gttctcatca cccacgccat
     3061 ggtcctcacc ccgttctgtc actcgatgta tcgtgcgccg atatactagt tacggcatgg
     3121 taggctgccg ctggaaggtt gtcaaggggt tatgttcctg gagtacgtca ctatctcgtg
     3181 gttggaacga cgctgcgagg cagaatctgg agtctttcat ctactgcgtg actggatcgg
     3241 ggcatcggcg ccatcggggc cggaattagc tacggtccga cgaccagcac ggcgacgcta
     3301 gattcgagct cccgactcca ggtaccaggc aacggtctct accgagtttg aagaccggtt
     3361 tgcagcaaat ggcgtggacg tccaccatca gagatcagag cggccatcac tacttttcgg
     3421 cgtagccctg tcccgcctcg attatccggc gcatctccgt cgccgggtct cctttgaaca
     3481 ccgcggagcc cgcgacgagc gcgttggccc cggcctcggc cgccacgggc gccgtctcga
     3541 ccgtgatgcc gccgtctacg gcgatcggca ggcccgtcat ctctttcaag cgacgaatct
     3601 tttctgctgc ctcgggaagg aacgattgcc ctccaaagcc cgggt
//
LOCUS       DBNGVD010000009         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4737923, whole genome shotgun sequence.
ACCESSION   DBNGVD010000009 DBNGVD010000000
VERSION     DBNGVD010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4737923"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16CM_00230"
     CDS             <1..290
                     /locus_tag="AC16CM_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138281.1"
                     /translation="ALPEGIVHELEELSPLAGGERIGAPVELATGPHDAYFPPSESYA
                     LRRVAPQRRVTVTKALDHAELQVSPADVPAFLVFDGFVVRALLISRDDGDS"
     gene            547..1977
                     /locus_tag="AC16CM_00235"
     CDS             547..1977
                     /locus_tag="AC16CM_00235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3138282.1"
                     /translation="MALGGALLDNIPEMVTISDRTGRITYANPATERVSGYKPEEFAS
                     LHPTERIHPDDLSRCEEAFGELLHTPGLSLELEHRAKHKDGTWRWVEVTLQSLFDDPE
                     VGGLLATVRDVTGRKRAETALAASEEQYRTLFASIDEGFCTIEVLFDERGDPVDYRFL
                     RMNPAFERQTGFVNAAGRTARELVPEHEEFWFEVYGRIARTGEAMRFEHEAAALGRYY
                     DVYAFRVDEPEKNRVAVIFKDVLARKRAEEEQERLRSLEASARAEAAERERIGRELHD
                     RVAHTMAVAHQSLQLHGALADRDPVRAAETLKRAAEATRTALDQTRHLSSQLARQNTE
                     ETRYGLSAALRGLLETHVPEGVEADLSVSGDEQVVSPRAAEEAYLVMREAVRNAVAHS
                     GCGRIDVGLEIDDAELKGHVRDDGTGFDRLESPNRVRGGREDGYAEGVGLRSMKERTG
                     QLGGRLEVHSEPGVGTTVEVRVPLVG"
     gene            complement(2006..2938)
                     /locus_tag="AC16CM_00240"
     CDS             complement(2006..2938)
                     /locus_tag="AC16CM_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR3138283.1"
                     /translation="MSQKPVILDVDPGHDDAVAMMLACGHPDLDLLAVTTVAGNVSLD
                     KTTRNALRVLSLIGCDDVPVGMGASGPLERPLYTAEDIHGESGLDGPEEIPDAGFGPD
                     ERDAVALLSDTIRASSEPVTLIPLGPLTNIALLLREHRDLQDRIAGISLMGGSMGLGN
                     TTPAAEFNIYVDPEAAREVFESGLPITMVGLDVTHQAGAGPEERERLRATGRVGGVVA
                     GFLDFFASTYERSFGFDSPPLHDPVAVAAVVEPALLETRPMRVDVECESDLTRGETVC
                     DFHGVTGKRPNAEVGVELDREGFFELLYGALGRV"
     gene            complement(2973..3656)
                     /locus_tag="AC16CM_00245"
     CDS             complement(2973..3656)
                     /locus_tag="AC16CM_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR3138284.1"
                     /translation="MDDADPRLSRLVLLCAALGAGLHLVASIRARGLRRSVLLLVLCT
                     GLPTTGELLATGPLRLLRHRLRYRLVGVPLAILLGWYAAIHGCLVIARRVAEPTRSGK
                     DATKTVPVLAALVGVSLDLILDPAGLDLGLWEWNSDGTYAAEVAGPNGHAGVPLVNYL
                     GWASLVGGATYFYGSACEDKAEDPLPALLLLPLYLAAVGWAIERRKYRYILLSATFPV
                     ALYVALQKG"
BASE COUNT          656 a         1240 c         1348 g          585 t
ORIGIN      
        1 cggccctccc cgagggaatc gttcacgagc tcgaagagct ctcgccgctc gcgggaggag
       61 agcgtatcgg cgcccccgtc gagctcgcca cgggccccca cgacgcgtac ttccctccct
      121 ccgagtcata cgccctccga cgcgttgcgc cgcagcgcag ggtcaccgtc acgaaggcgc
      181 tcgaccacgc cgaactccag gtgtcgccgg cggacgtccc ggcctttctc gtcttcgacg
      241 gcttcgtggt ccgcgcccta cttatctcac gagatgacgg cgattcgtag gaaatcgagc
      301 ctcgtcgcga gggaaggatg cgagttacgc gttacgggct tcggatccct gatgcccgaa
      361 actctccctc gggttcgccg tttccggacc caccatggtt ctggatttgc acgaaggcga
      421 gggctccgca cgttataatt cggaacgaga tcgggtgcct gggtagtacg tacgggaggt
      481 cttatggtgg gcgacacggg cggggctgca tccggccgac ggtcgtccgc ggataggaga
      541 cgtgacatgg cgctgggtgg tgccctgctc gacaacatcc cggagatggt caccatcagc
      601 gatcgcacgg gcaggatcac ctacgccaac ccggccaccg agcgcgtctc cggctacaag
      661 ccggaagaat tcgcctccct ccaccccacg gagcggatcc atcccgatga tctctcccgc
      721 tgcgaagagg ccttcgggga gctgttgcac acccctggcc tgagcctcga gctcgagcac
      781 cgcgcgaaac acaaggacgg gacgtggcgc tgggtggagg tgacgctaca gagcctcttc
      841 gatgaccccg aggtaggagg gctgctcgcc accgttcgcg acgtgaccgg gcgcaagcgc
      901 gccgagaccg ccctggccgc ctctgaagag cagtaccgca cgctgttcgc ctccatcgac
      961 gagggcttct gcaccatcga ggttctcttc gacgagcggg gcgatccggt cgattatcgc
     1021 tttctccgga tgaacccagc cttcgagcgg cagaccggct tcgtaaacgc cgccggccgg
     1081 accgcgcgcg aactcgtgcc cgaacacgag gagttctggt tcgaggttta cgggcgtata
     1141 gcccggaccg gcgaggcgat gcgcttcgag cacgaggccg ccgcgctggg taggtactac
     1201 gacgtgtacg ccttccgcgt ggacgagccg gagaagaacc gcgtggccgt aatcttcaag
     1261 gacgttctgg cacgcaagcg ggccgaggag gagcaggagc ggctgcgctc tctggaggcc
     1321 tcggcccggg ccgaggccgc ggagagggag aggatcggcc gcgagctaca cgaccgcgtt
     1381 gcccacacca tggcggtggc ccaccagtcc ctacagctcc acggggcgct ggccgacaga
     1441 gaccccgtcc gcgcggccga gacgttgaag cgggccgcag aggccaccag gacggccctt
     1501 gatcagaccc gccacctctc ctcccagctt gcccgccaga acacggaaga gacgcgctac
     1561 gggttgtcgg cggccctgcg cgggctgctc gagacccacg tgccagaggg cgtggaggcg
     1621 gacctctcgg tgtccggtga cgagcaggtg gtctcgccgc gcgcggccga ggaggcgtat
     1681 ctggtgatga gggaggccgt gagaaacgcc gtggcacact ccgggtgcgg gcggatagac
     1741 gtgggcctcg agatagacga cgcggaactc aagggacacg tgcgagacga tggaacgggc
     1801 ttcgacaggt tggaatctcc gaacagggtg cggggtggcc gggaagacgg ttacgcggag
     1861 ggggtgggcc tgaggtccat gaaggagcgg accggacagc tcggcggacg gttggaggtc
     1921 cactcggagc caggggttgg taccacggtg gaggtgcggg tcccgctggt ggggtgaggg
     1981 ccccactctg gcgccaggat ctcttctaca cccgtcccag cgccccgtag agaagctcga
     2041 agaagccttc cctgtcgagc tcgaccccga cctcggcgtt cggcctcttt cccgtaacgc
     2101 cgtggaagtc gcagacggtc tcgccgcggg tgaggtcgct ctcacactcg acgtccaccc
     2161 gcatcggtcg cgtctctagc agggcgggct ccacgacggc cgcgacggcg acggggtcgt
     2221 gcaagggcgg tgagtcgaag ccgaaggagc gctcgtaggt cgaggcgaaa aagtcgagaa
     2281 agccggccac gacgcccccc acccggcccg tcgcccgcag ccgctcccgt tcctcgggac
     2341 cggcgccggc ctggtgtgtg acgtcaagcc cgaccatggt tatgggcagg cccgactcga
     2401 agacctcgcg ggcggcctcg gggtcgacgt agatgttgaa ctcggccgct ggcgtcgtgt
     2461 tgcccaaccc catgctccca cccatcaggg agatgcccgc tatgcggtcc tggagatccc
     2521 gatgttcgcg caggagcagg gcgatgttgg tgagcgggcc gagcgggatg agggttaccg
     2581 gctcggatga ggcccttatg gtgtccgaga gcagggccac cgcatccctc tcgtccgggc
     2641 cgaagcccgc gtcggggatc tcttccggcc cgtctagccc gctctcgccg tggatgtcct
     2701 cggcggtgta gagcggcctc tccaggggcc cggaagcccc catcccgacg ggcacgtcgt
     2761 cgcacccgat gagggagagg acgcgcaggg cgttgcgcgt ggtcttgtcg agggagacgt
     2821 tgccggcgac ggtcgtgacg gcgagcaggt cgaggtcagg gtgcccgcag gccagcatca
     2881 tggccacggc gtcgtcgtgg ccggggtcta cgtcaagtat cacgggcttc tggctcacct
     2941 tggctcctct cgatctttta caggatggta acttagccct tttggagcgc cacgtacagc
     3001 gcgacgggaa acgtcgccga gaggaggatg taccggtact tgcgccgctc gatggcccac
     3061 ccgaccgccg ccaggtagag gggtaacaag agcaacgcgg ggagagggtc ctccgctttg
     3121 tcctcgcaag cagacccgta gaagtaggta gcgcccccga ccagcgaggc ccagcccagg
     3181 tagttgacga gcggcacccc cgcatgcccg ttcggaccgg ccacctccgc tgcgtaggtg
     3241 ccgtcgctgt tccactccca gaggccgagg tcgaggccgg ctgggtccag gatgaggtcg
     3301 aggctgacac cgacgagggc agccaggacc gggacggtct tcgtggcatc cttgccggaa
     3361 cgggtgggct ccgcaacccg cctggcgatg acgaggcagc cgtggatggc ggcgtaccag
     3421 ccgaggagga tggcaagggg cacgcccacc agcctgtagc gcagccggtg gcgcagcagc
     3481 cgcagggggc ccgtggcgag gagctcgccc gtggtcggta gcccggtaca gagcaccagg
     3541 agaagaacgg agcggcggag gccgcgggcc ctgatcgagg ccaccaggtg caggccggcg
     3601 ccaagggcag cgcagaggag gaccagcctc gataacctgg gatctgcgtc gtccaaagag
     3661 ggacctccaa gccaaaccca tccgagcgtc tacagcatac gccgctggag gggcgttatc
     3721 ttctcttgtt gacgaccgga cttttgttgg gtaaagtcat tgccaatggg gagtagccac
     3781 ccgctttagc ggggggaccg gtcgtcaata cggggcgaag cccccggcc
//
LOCUS       DBNGVD010000010         2275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4191662, whole genome shotgun sequence.
ACCESSION   DBNGVD010000010 DBNGVD010000000
VERSION     DBNGVD010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4191662"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="AC16CM_00250"
     CDS             <1..1211
                     /locus_tag="AC16CM_00250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3138285.1"
                     /translation="VPDFLSGQTQKAVYRNDQEAALIWYHDHALGITRLNVMAGLAGA
                     YLVRDSVDNGTGGNFTAFGGGTLPRGNYEVPLVIQDRAFAKDGRIKYPAQWTPEFFGD
                     VVCVNGKVWPFKTVGPGKYRMRILNGSNSRFYNLRLTGGATMTQIGTDGGFLQAPVPI
                     PRLLIAPGERADVVFDFTGTGGNVDLIDTPLPPTTVSPSTPLRRSEIMRFVVDPAIPV
                     PDVTLPTQLRAEAYSVPGIVAQERWLTLEERLAGPVPTALLLNGMRFGDRPVTEQPNL
                     DDTEVWTLINVSADSHPMHLHLPQFEVLERRRLDVAGYQSALDAARAASGGPVDANGN
                     LVNPDPTPYLGAEEPVDDNEKGPKDTVRANPAQITRIKVNFDIAGKYVWHCHILEHEE
                     NDMMRPLDVV"
     gene            1563..1898
                     /locus_tag="AC16CM_00255"
     CDS             1563..1898
                     /locus_tag="AC16CM_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HIR3138286.1"
                     /translation="MERLLIIGALFVLAGLCEIGGGYLVWGWVRDHKPLAWALIGALI
                     LAAYGVVAALQPIPEFGRVYAAYGGVFIALALAWGVLVDGFRPDRWDLLGAAICVAGV
                     VVMVAPSRG"
     gene            complement(1909..>2275)
                     /locus_tag="AC16CM_00260"
     CDS             complement(1909..>2275)
                     /locus_tag="AC16CM_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138287.1"
                     /translation="RSEKPEQGVPMTDHEESKLERYIPLACDMSALDAGQRRRQGVLA
                     RRLRDGALEIRELANGYAFAHPPDADTLAMMAEFVANERLCCPFFGFEISVGGDGPAW
                     LGIAAEGEAKGILEAEMGI"
BASE COUNT          360 a          777 c          744 g          394 t
ORIGIN      
        1 tcgtgcccga tttcctctcg ggtcagacgc agaaggccgt ctaccgaaac gatcaggaag
       61 ccgccctcat ctggtaccac gaccacgccc tcgggatcac gcgcctgaac gtgatggcgg
      121 ggcttgccgg cgcctacctc gtgcgcgaca gcgtcgataa cggcacgggg ggcaacttca
      181 ccgccttcgg cggcggcacg ttgccgaggg ggaactacga ggttcccctc gttatccagg
      241 acagagcgtt cgccaaggac ggtaggatca agtacccggc ccagtggacg cccgagttct
      301 tcggcgatgt ggtctgtgtc aacggcaagg tgtggccctt caagacggtc gggcctggca
      361 aataccgcat gaggatcctg aacggttcca actctcgctt ctacaacctg cggctcaccg
      421 gcggggccac gatgacccag atcgggacgg acggcgggtt cctccaagcc ccggtgccga
      481 tacccaggct cttgatcgcc cccggcgagc gggccgacgt cgtcttcgac ttcacgggaa
      541 ccggcggcaa cgtcgatctt atagacaccc cgctcccgcc cacgacggtc agcccctcca
      601 ccccgctgag acgatccgag atcatgcgct tcgtggtgga cccggcgatc ccggtgcctg
      661 atgttacgct gccgacgcag cttcgtgcgg aggcgtactc agtaccaggt atcgttgcgc
      721 aggagcggtg gctgacgctg gaggagcggc ttgcgggacc cgttcccacc gccctgctgc
      781 taaacggcat gcgcttcggg gaccggcccg tcaccgagca gcccaacctc gatgacacgg
      841 aggtgtggac cctgatcaac gtctcggccg actcccaccc catgcacttg cacctgccgc
      901 agttcgaggt cctcgagcgt aggcgactcg acgtggccgg ctaccagtcg gcgctcgacg
      961 cagcccgcgc cgcctccggt ggcccggtcg acgccaacgg caacctcgtc aatccggatc
     1021 caacccccta cctcggggcg gaggagccgg tggacgacaa cgagaagggc ccgaaagaca
     1081 ccgtgcgggc gaacccagcc cagatcacca ggatcaaggt gaacttcgac atcgccggca
     1141 agtacgtgtg gcactgccac atcctcgagc acgaggagaa cgacatgatg cgcccgctcg
     1201 acgttgtcta gggtggcggc tccggggtcg agatgggccg aggctgcttc tctatacgca
     1261 cggcggtccc ggaagggtga tggatccgcc acgagggtag cctgaacagg gccgcatgct
     1321 cgctagcgga ggaccgcctg gatggaaggg tcaggacgtg tgagtcctgg cccttctcct
     1381 tgcccttgcg caccaaaacg gaagcctccg cgcctacacg gtctcggcct cgtcgtccgc
     1441 gttgatccac tccaccatgc gccgcacctt cgccttgtcc tcctcggaca cgcccggcgc
     1501 tcctttcacc gcgggtggct acactctgcc ccggtgccac gtagtctaac gggaggtgcg
     1561 gggtggaacg tctgcttatt atcggtgcgt tgttcgtgct ggctggcttg tgcgagatcg
     1621 ggggtggcta cctcgtgtgg gggtgggtgc gagaccacaa gcccctggcc tgggcactga
     1681 tcggagcgct gatcctggcg gcctacgggg tggtggccgc cctccagccc atcccggagt
     1741 tcgggcgggt gtacgccgcc tacggcgggg tgttcatagc cctggcgctg gcctggggcg
     1801 tccttgtgga cgggttccgg ccagaccgct gggacctgct cggcgcggcg atctgcgtgg
     1861 ctggcgtggt ggtgatggtc gccccgtcca ggggatagcc gcccgggctc agatgcccat
     1921 ctccgcctcc aggatgccct tggcttcccc ttccgccgcg ataccgagcc acgccggccc
     1981 gtcaccgccg accgagatct cgaacccgaa gaacgggcag cacagcctct cgttggcgac
     2041 gaactcggcc atcatggcga gggtgtccgc gtccggcggg tgcgcgaagg cgtagccgtt
     2101 ggcaagctcc ctgatctcga gcgccccgtc gcgtagtcgg cgagccagca cgccctgacg
     2161 ccgccgctgt ccggcgtcga gcgccgacat gtcgcaggct agggggatgt accgctccag
     2221 cttggactct tcatggtccg tcatgggtac tccttgctcc ggtttctcag acctc
//
LOCUS       DBNGVD010000011        12028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1421, whole genome shotgun sequence.
ACCESSION   DBNGVD010000011 DBNGVD010000000
VERSION     DBNGVD010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1421"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="AC16CM_00265"
     CDS             complement(<1..1322)
                     /locus_tag="AC16CM_00265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3138288.1"
                     /translation="MSKVERPVILVVDDEPAVLSALVRDLQREYGQHYRVVRAASGEA
                     ALEAVRELKLRAVPVALFLVDQRMMGMSGVEFLENAIEVFPDAKRVLLTAYADTQAAI
                     SAINDVALNHYLMKPWNPPEDNLYPVLTDLLDDWQASYAPPFEGIRVVGHQLSAQSHR
                     VKDFLSRNLVPYQWLDAEVDEEAGRLLELSSSDSSRLPLVLFPDGPSLEQPSNVQLAE
                     RIGLKMHPGLPFYDLVIVGAGPSGLAAAVYGASEGLGTLLIEGDAPGGQAGTSSRIEN
                     YLGFPSGLSGGDLSRRAVAQAIRFGAEILTPLEATCARAEGPYRFVTLTDGSEVSCHA
                     LLIATGVSYRKLDVPGVERLTGAGVYYGAAITEALACRDRDVFVLGAGNSAGQAAIHF
                     SRYASSVTMLVRGASLDRTMSQYLVSQIETHEKITVSPRTTVVGVTGGG"
     gene            1671..1910
                     /locus_tag="AC16CM_00270"
     CDS             1671..1910
                     /locus_tag="AC16CM_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138289.1"
                     /translation="MRRMIKALAMATLVATILVASTSPALARVVRGGVLLHTERPCHA
                     TLVAQNAPGSHILLNPEDRTPGCWVELPPGANHDL"
     gene            complement(1966..4731)
                     /locus_tag="AC16CM_00275"
     CDS             complement(1966..4731)
                     /locus_tag="AC16CM_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138290.1"
                     /translation="MAKPHSGTITFLFTDLEGSTSLWEKNPEAMSEALYRHDEILRTT
                     IEAHNGRVFKTVGDAFYAVFSAAPDALRAALEAQRILVREEWAETGPFRVRMALHTGI
                     AQERDGDYFGPSLNRVARLLSAGHGGQILTSLATRELVRDGLPEGTGLLDLGERLLKD
                     LSRPEHVFQVTAPDLPSEFSPLKTLEGRHSTLPAQPTPLVGRDRELEQVLALLRSPAV
                     RLLTLTGPGGTGKTRLGLQAATELTDEFEDGVFFVALAAIADPALVAPTIARTLGLAE
                     GSIQPPEEILEGYLRDRQTLLVLDNFEQILASAALLDALLSAASGLKVLATSRTPLGL
                     YGEHEYPVPPLSLPAPGHLPSTERIARYEAIRLFVERARAVRPDFSLTEENAAAVVEI
                     CARLDGLPLAIELAAARTKLLSPQAMLPRLSNRLRLLTGGARNLPERQRTLRNAIEWS
                     YEMLDEGERMLFARLAVFSGGSTLEGIEAVCDAAGDLPVDPLEGVSSLLDKSLLRQDE
                     GVEGEPRFEMLETIHEFALDKLGASGDAGATRRAHAEYFLALAERAEPRLWGPEDAAW
                     LDRLEEEHDNMRAALSWALDNREAEVALGLGGALRWFWNMKGYYSEGRSWLAAALTAD
                     GGASALSRAKTLEGLGWLASQQGDLDEAETIAGDGLRLGAEAELGDVVSADFRNVLGD
                     AARQRGDYERAAELLEEGLTLHRKTEDVRGVAWSLGNLANVSTDRGNYEQAKRLYEEG
                     LALSRDLGGAELLGAYLISLGYECLLEGDPERARELNEEAVALYRGRGRKGNLQVALD
                     NLGWAALIEGDHERSEALFEECLVLCRDLGDRLNGSESMEGLACAAGARGEAGRAARL
                     FGAAEALREAAGYQQATRARSLREPYLAAARSGIGGDYWTKAWESGRSMTFEDAIAYA
                     LERNANS"
     gene            complement(4811..5584)
                     /locus_tag="AC16CM_00280"
     CDS             complement(4811..5584)
                     /locus_tag="AC16CM_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3138291.1"
                     /translation="MGRLIFLGTGDSLNPERAQTSLAVLLPGDEAMLIDASSGTVLLG
                     RLEACGIPLESVRHLFISHRHFDHVGGLAPLLTALAALPEAFLTVHAAPETLRALRGV
                     LELTIPGAEGWLGKRLGWHELLPNEPTELEGTDVIPFFMNHGIECLGFRIAQMSSVAV
                     VAGDTRPSPEIVERAGGADLLVHDAYGPQSAAQQAHAMGHSTATEAGQAAHAAGVKRL
                     ILTHLRASRFADPDALAAEAEAAFGGPVDAANDLDVFDF"
     gene            complement(5798..6352)
                     /locus_tag="AC16CM_00285"
     CDS             complement(5798..6352)
                     /locus_tag="AC16CM_00285"
                     /inference="COORDINATES: protein motif:HMM:NF040152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HIR3138292.1"
                     /translation="MGAAEDGSPGKQTMERVDLMDAREELIEVLLRESREELERIDLK
                     ASILLSVCSLALAALVHAAAYLHWDPRELLVFQWFLWLGMAFGGAALVALAAAVWPRL
                     GHGQGEITYFGHVAQFEGIEELNRALDQEVSANPSRTDHVAKRLLAMSRIIYDKYRYI
                     RWGMGLFGVAVLLCTVAAVRALTT"
     gene            6716..7888
                     /locus_tag="AC16CM_00290"
     CDS             6716..7888
                     /locus_tag="AC16CM_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HIR3138293.1"
                     /translation="MAEEGSDDRSLSRERRLQLLTGVPALAHLPTSALEGLAAGLEEE
                     RFPLDGIVVAEGDAEDRLYLIVEGRARASTTGSSEPVPLALMGPGELFGEIALLEPGG
                     RRQATVIAVEPLLLLSLRAADFRRLLEEHPEVHASFAKVADDLRVTKFLKQVSPFSML
                     DGERLRRLVSRLERSEVPAGTTIISQDDFGEECYLLQSGRVEVLARGVSGDENHLATV
                     GPGSLLGEAALLTDEPRNATVKALEPCTLLALRRADLLESIGEDRQTGQRVLELVHLH
                     DRPRRVSGMEAHHRTTILGESITTLRDPRRDVYYRLSAIGWFLWQRLDGQHAVQDLTS
                     EYTAAHGALPPHAVVETVAGLVEAGFARGANPGPELPGDRPRLWQRAVAAIRRVLE"
     gene            8423..8824
                     /locus_tag="AC16CM_00295"
     CDS             8423..8824
                     /locus_tag="AC16CM_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138294.1"
                     /translation="MMTKVRASVWVLAVLVIAGSAAGCGQVSDRAQQEAEKKVENKGQ
                     QAREEAKEKLNAKQQEAKEKLEAGREDLERKVDDLKKEVNDLQKDVATLQKKIHAQEQ
                     KEQDQQINQLKKALKKLQKKVEAQGQQGQKN"
     gene            9060..9626
                     /locus_tag="AC16CM_00300"
     CDS             9060..9626
                     /locus_tag="AC16CM_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR3138295.1"
                     /translation="MNSNAGASAEALPWWLVLVEGIVAALFGLILLVAPGASLVFLVW
                     LLGIYLLVAGVVRIVGIFLDSSSWGWKLVAGILCLIAGLAILSNPLWSTTLASTWLVI
                     LVGFLAMLQGAAGLVVAFQGGGWSMGALSVLGILLGLFLVINPLIGAAALTLILSIFM
                     LIGGVGAVVQAFRMRSGSATVQRPPRVR"
     gene            10031..10168
                     /locus_tag="AC16CM_00305"
     CDS             10031..10168
                     /locus_tag="AC16CM_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138296.1"
                     /translation="MSGVMITKIRDGKAVRGWFYWDQLARLVQLGITEQPGHFLSAAG
                     F"
     gene            complement(10174..11046)
                     /locus_tag="AC16CM_00310"
     CDS             complement(10174..11046)
                     /locus_tag="AC16CM_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3138297.1"
                     /translation="MRGVVELGRHSVRSFFEHRMAIYAEQLSYRGLFGFFPFIILVFA
                     LLAVLRIDAVFDRLIEAAMDVPPQEIPEPIEPMAEKGRNQAAFLLPLIQQAREQAGGG
                     LLSFGLVISLWSVSAVIFTLMEALNAAYGVEETRSRRKRFAFALVFGPLLALAFIAAC
                     GLMLIGPRVAGWVAGVADLDEAWVAFWGWLRFPVALLLLSAVLAVVYRFVPDADRSFW
                     SATPGAVFAVIAWPIASVGFSIYLSDFAGRGLTYGSLGTAVGLLVFLYISAWIVLLGA
                     EINEAVYRGYQRQT"
     gene            complement(11207..11968)
                     /locus_tag="AC16CM_00315"
     CDS             complement(11207..11968)
                     /locus_tag="AC16CM_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR3138298.1"
                     /translation="MGLENPALNERSFRRGIEEAASEPRGMSASGTYAIVGLLFVIVV
                     AAAAFGWSQVEIVSVGGRKEALTPAWTWLAFLLTFVVAIVGAFAYRAAPITSVLYALG
                     EGSLLGIASRYYDLRFDGIVSQALLATLCIFVAVYLLYSLRIVKVTSRFATGVMAATG
                     GFVLLYLVVWLLSLFGVDFRFLYTPTPLGIGISVLIVILGALNLTLDFAFIERSAEAG
                     APRFMQWYGAFGLMLSLIWIYVSVLRLLVLLRSRQ"
BASE COUNT         2301 a         3802 c         3737 g         2188 t
ORIGIN      
        1 ccgccgccgg taactcccac gaccgtagtt cgcggcgaca cggtgatctt ctcgtgcgtc
       61 tcgatctgcg agacgaggta ctgggacatc gtcctgtcta gcgaggctcc cctgacgagc
      121 atggtcaccg agctggcgta ccgcgagaag tgtattgcgg cctgaccagc tgaatttccc
      181 gctccgagga cgaacacgtc cctgtcccga caggccagag cctcggttat agctgccccg
      241 tagtacactc ctgcaccggt gagcctttcg acgccgggca cgtctagctt gcggtacgac
      301 acgcccgtag ctatgagcag ggcgtggcaa ctgacctcgg acccgtccgt gagggtgacg
      361 aaacggtagg ggccttctgc cctcgcgcac gtcgcctcca ggggcgtgag tatctccgcg
      421 ccgaaacgga ttgcctgggc cacggctcgt ctcgagagat cccccccact caagccgctc
      481 ggaaaaccca ggtagttctc gatgcgggag ctcgtacctg cctgtccgcc gggcgcgtcg
      541 ccttcgatga gtagtgtgcc gagcccctcc gaagccccgt agaccgcggc tgccaaaccg
      601 gacggaccgg cgccaacgat gaccaggtcg taaaatggca agccggggtg catcttcagg
      661 ccgatcctct ccgccagttg cacgttcgag ggttgctcca gggaagggcc atcgggaaaa
      721 agcaccaggg gcaacctcga gctatccgag cttgagagct cgagcaaacg cccggcttcc
      781 tcgtcgacct ccgcgtcgag ccactgatag ggaacgaggt tcctggaaag gaagtccttg
      841 accctgtgcg attgggcaga gagctggtgg cctacgacgc ggatgccttc gaacggcggg
      901 gcgtaagagg cctgccagtc gtccagtagg tccgtgagca cgggatagag gttgtcttcc
      961 ggaggattcc acggcttcat aaggtagtgg ttcagggcca catcgttgat cgcgcttatg
     1021 gcagcctgag tgtctgcata cgcggttagg agcacacgct tggcgtcggg gaagacctct
     1081 atggcgttct cgagaaactc cacgcctgac atgcccatca ttctctggtc caccagaaac
     1141 agggcgacgg gcacagcgcg gagcttcaat tcccgcaccg cctcgagcgc cgcttcacct
     1201 gaggcggccc tcaccaccct gtaatgctgt ccatactcgc gctgcaggtc acgcacgagg
     1261 gcgctcagga ccgctggctc gtcatcgacg acgagtatta ccggcctctc taccttgctc
     1321 acgggattca caaattctcg accatcgcac gtccgttgag cccggcgcac cggtgcgcac
     1381 gctcgaacgg tcgcgaggcc ctgcctagcg taactgccac cgtgtgtttc ttcacgtcca
     1441 tctcgccaac cggatgggaa cggcgtcaag cccatccgtg tttactatag cagtggaatt
     1501 taccgcaaaa gggccacgac caggagaacg gattcggaca cgcgagcaga cgcatcgccg
     1561 agcactaccg gctgagcccg tcgggctccg tcctataccg ctacgtgatc cgttctgtta
     1621 tcgtggctgt ggtggaaagg tgaggagcgc acagagcaag gaggtagcct atgaggcgta
     1681 tgatcaaggc cctggcgatg gcaactcttg tggcgacgat tcttgtggca agcacttccc
     1741 ccgcgctggc cagggtcgtg cgaggtggcg tgttgctgca cacggagagg ccctgtcacg
     1801 ctaccctggt ggcccagaac gctcctggat ctcacatact gctcaacccg gaagatcgga
     1861 cgccggggtg ctgggtggaa ttgccacctg gcgccaacca cgacctttga tccggtgtag
     1921 gggcgtagat gccgttcatg tgcagtcgcc tcctcgggag ttggactagg agttggcgtt
     1981 cctctccaga gcgtaggcga tagcatcctc aaacgtcatg gagcgcccgc tttcccacgc
     2041 ctttgtccaa tagtccccgc ctattccgga gcgggcggct gcgaggtagg gctcccgtag
     2101 agagcgggct ctggtcgcct gctggtagcc tgctgcctcg cgcaacgcct ctgcggcccc
     2161 gaacagcctc gccgcccgcc ctgcttctcc cctggctccg gcggcacacg caagcccctc
     2221 catgctctcc gatccgttga gcctatcgcc cagatccctg cacagcacga ggcactcttc
     2281 gaagagtgct tcagatcgct cgtgatctcc ctcgatcagc gctgcccatc ccaggttatc
     2341 gagggcaacc tgaagattgc ccttgcgtcc ccgcccccgg tacagcgcca cggcttcctc
     2401 gtttagctct ctcgccctct cgggatcgcc ttcgagaagg cactcgtaac cgaggctgat
     2461 caagtaggca ccgagcaact ctgcaccgcc caggtccctg gagagggcca ggccttcttc
     2521 gtacaggcgc ttggcctgct cgtagttacc acggtcggtc gagacgttgg ccaggttgcc
     2581 aagagaccag gcgacgcctc taacatcttc ggtttttctg tgaagcgtca aaccctcctc
     2641 cagcaactcg gccgcccgct cgtagtcacc cctctgcctc gccgcatccc ccagtacgtt
     2701 ccggaagtcg gccgatacta cgtctcccag ctctgcttct gcgcccagcc tgaggccatc
     2761 tccggcgatc gtctccgcct cgtcgagatc accctgctgg ctcgccagcc agcccaggcc
     2821 ctcgagtgtc ttggcccgcg acaacgctga cgctcccccg tcagcggtca gtgccgccgc
     2881 aagccagctt cttccctcgc tgtagtaccc tttcatgttc cagaaccacc gcagcgcccc
     2941 acctaatcca agggcaacct cggcctcccg gttgtccagg gcccaggaga gtgccgccct
     3001 catgttgtcg tgttcttcct ccagccggtc cagccacgcc gcatcctcgg gcccccacag
     3061 cctcggctcg gcccgctctg ccagggccag gaagtattcg gcgtgggcgc gccgcgtagc
     3121 gcctgcgtca ccgctcgccc caagtttatc caacgcgaac tcgtggatgg tctcgagcat
     3181 ttcgaagcgc ggctcgcctt cgacaccttc atcttgccgc aacaaactct tatccagtag
     3241 tgaagagacc ccttcgaggg ggtccacggg gagatctccc gccgcatcgc agacggcctc
     3301 tatgccctcc agggtgctcc cgccggagaa gaccgcaagc ctcgcaaata gcattctctc
     3361 cccctcgtca agcatctcgt agctccactc gatggcgttc ctgagggttc tctgcctctc
     3421 tggcaggttc ctcgcccccc ccgtcagcag cctgaggcgg ttgctcaagc ggggcagcat
     3481 cgcctgggga gagagcagct tcgttctcgc cgccgctagc tctattgcca gaggcagacc
     3541 gtcgagcctc gcgcaaatct ccaccaccgc agcggcattc tcttccgtca acgagaagtc
     3601 cggtctgacg gctctggctc gctccacgaa cagcctgatc gcttcgtagc gggcgatacg
     3661 ctccgtggat ggtaggtgcc cgggggctgg cagtgagaga ggcggaacgg ggtactcgtg
     3721 ttcgccgtag agcccgagag gggtgcggct ggtggcgagt accttcaggc cggatgccgc
     3781 cgagagcaag gcgtccagta gcgccgccga cgcgagtatc tgctcgaagt tgtcgagaac
     3841 gagcaacgtc tgcctgtcgc gcagatagcc ttcgaggatc tcctccggcg gctggatgct
     3901 gccctcggcc agccccaggg ttctggcgat ggtcggggcg acgagcgcgg gatccgcgat
     3961 cgcggcgagc gcgacgaaga acaccccgtc ttcgaattca tcagttagct cggtggcagc
     4021 ctgcaagccg agccgcgtct tccccgtccc gccaggaccc gtgagggtca gcagtcgcac
     4081 agcggggctg cggagcagcg ccagcacctg ttcgagttcc ctgtcacgtc ccaccagcgg
     4141 ggtgggctga gcggggaggg tgctgtgccg accttccagc gtcttgagtg gcgagaactc
     4201 cgaaggaagg tctggggcgg tgacctggaa gacgtgttcg ggcctggaga gatcctttaa
     4261 gagtctctcg cccagatcca gtaaccctgt cccttctggc aacccgtctc gcaccaattc
     4321 cctggtggcc agggaggtca gaatctgtcc tccgtgcccc gcggagagga gcctggcgac
     4381 cctgttcaag gaaggtccga agtaatctcc gtctcgctcc tgggctatcc cggtgtggag
     4441 cgccatcctc acccgaaagg gtcctgtttc ggcccactct tcgcggacaa ggatcctctg
     4501 ggcctcgagt gccgcccgaa gggcatcggg agcggccgag aagacggcgt agaaggcgtc
     4561 gccgacagtc ttgaaaaccc ggccgttgtg ggcctctatg gtcgtgcgaa gtatctcgtc
     4621 gtggcgatag agagcttcgg acatcgcctc cgggtttttc tcccagagcg aggtggagcc
     4681 ttccaaatca gtaaacagga aggtgatcgt gccgctgtgt ggcttcgcca tccgctccgc
     4741 aaccttctat cccgatgaga acaattaaag caccccagcg acgaacggcc agcgcccgag
     4801 ggcagcccgc tcagaaatcg aagacgtcaa gatcgttcgc cgcgtcgacc ggccccccga
     4861 aggcggcttc ggcctcggct gccaacgcgt cggggtctgc gaagcggctg gcgcggaggt
     4921 gggtgaggat gaggcgcttc acgccggccg cgtgggccgc ttgaccggcc tcggtcgccg
     4981 tcgaatgccc catggcgtgt gcctgttggg cggcgctttg cggaccgtac gcatcgtgga
     5041 cgagcaagtc ggccccgccc gcacgctcga cgatctccgg actcgggcga gtatcgccgg
     5101 cgacgaccgc cacagaactc atctgggcta tgcggaagcc caggcattcg atgccgtggt
     5161 tcataaagaa ggggataacg tctgtccctt ctagctcggt aggctcattg gggaggagct
     5221 cgtgccaccc aaggcgcttc cccaaccatc cttccgcacc agggatcgtc agctcaagga
     5281 cccctcgcaa cgcgcgaagc gtctccgggg cagcgtgaac ggtgaggaaa gcctcgggaa
     5341 gggccgcaag ggccgtgagc agtggagcta gtccgccaac gtgatcgaag tgacggtgac
     5401 tgatgaacag gtggcgcacc gattcgagcg ggatgccgca agcctcgagg cgtccgagca
     5461 gtacggtgcc gctggaggcg tcgatcagca tcgcctcatc acccggaagc agcacggcca
     5521 ggctggtctg agccctctcc gggttcagcg agtcaccggt accaaggaag atcagacggc
     5581 ccactttgcc ctccctaaga ctcccgttag cgaacgaggt gccgactctg gcggacgttt
     5641 cttcgaggat ccgccccctt gcgtatagca caggaagatg cggcatgcga gtacacgggg
     5701 tcaagaggac ggtctcgcca atgctgcaca agacccgacc gtataccttc gggtcgctgg
     5761 gtcttgtcaa ctatacgcct ggaggtggaa gtgttcctca agtggtcaga gccctgacag
     5821 ccgccaccgt gcaaaggagc acggctacgc caaaaagccc catcccccat ctgatgtatc
     5881 tgtacttatc gtagatgatc ctgctcatcg cgagcaggcg cttcgccacg tgatccgtgc
     5941 gggaggggtt cgcggacacc tcctggtcga gggcgcggtt cagctcttct atcccctcga
     6001 attgtgcgac gtggccgaag taagtgatct ctccctgacc gtgaccaagc ctgggccata
     6061 ccgctgcggc caacgcaact agcgcggcac cgccgaacgc cattcccaac cacaagaacc
     6121 actggaagac caggagctcc cgcggatccc aatggaggta ggcggccgcg tggacgagcg
     6181 cggcgagcgc caggctgcag acgctcaaga gtatggacgc cttcaagtct atgcgctcca
     6241 gttcctctcg gctctctctc aggagcactt cgatcagctc ctcgcgagcg tccatcaggt
     6301 cgaccctctc catcgtctgc tttccgggag atccgtcttc tgctgcgccc atattgctac
     6361 ctccgctcgt ctcctatttt tgtagcttat cgtacaacct agcctcacaa aaagcgcgcc
     6421 gagggtctgg catcagcccg gacagatcta gacggttgaa ggcatgccac cctgtaactg
     6481 cccttctggg cgcctggatt cttcctgacc ttgtgaggac cattctctac ccgcaaagca
     6541 gctcaccttg ttatctggtg ccgccagcag cagggtaagg gtcgccctca cagagcattg
     6601 atccgcgcct tccttatcta gctctgcgag ctttggcttc tatctcttga tccggacgca
     6661 agatcgtgtt taatgattct gatgcagaac cgttgcaagc ccgacgggga tgttaatggc
     6721 cgaggaagga tcagacgatc ggagcctctc ccgcgagagg cgtttgcagc tactgacggg
     6781 tgtccctgcc ctcgcgcacc tgcccacttc cgcgctggag gggttggccg caggcctgga
     6841 ggaggagcgc tttccgctcg acggtatcgt cgtggccgag ggcgacgcgg aagaccgact
     6901 ctacctgatc gtggaggggc gggcgagggc ttcgactacg ggatcttctg aacccgtgcc
     6961 cctggcgctg atgggcccag gggagctgtt cggcgagatc gcgttgctcg agccaggtgg
     7021 caggcggcag gccacggtca tcgccgtcga acccctactg ctgctgagcc tgcgtgccgc
     7081 cgactttcga cgcctgttgg aggagcatcc ggaggtccat gcttccttcg ccaaggtggc
     7141 agacgacttg cgggtgacga agtttctcaa gcaggtgagc cctttctcca tgctcgacgg
     7201 cgagcgcctc cgtcggctcg tgagccggct cgagcgctcc gaagttccag ccgggaccac
     7261 catcatcagc caggatgatt tcggcgaaga atgctatctg ctccagagcg gtcgtgtgga
     7321 agtgctcgcg agaggcgtct caggcgacga aaaccacctg gccacggtcg gccccggttc
     7381 cctcctgggg gaggcagcgt tactgaccga cgaaccacgc aacgccacgg tgaaggctct
     7441 cgagccgtgc acgctgcttg cgctgcgccg cgccgatctc ctggagtcca ttggtgaaga
     7501 ccggcagacg ggccagcggg tgctcgagct cgtgcacctg cacgatcgcc cgcgtcgggt
     7561 atccgggatg gaagcccacc atcgcaccac catcctcgga gagagcatca cgaccctcag
     7621 agaccccagg cgcgacgtct actaccgtct atcggccata ggctggttct tgtggcagcg
     7681 actcgacggc cagcacgccg tgcaggatct gacgtcagag tacacggctg cgcacggggc
     7741 gcttccgccg cacgccgtgg tcgagacggt ggcaggactg gtggaggcgg gcttcgccag
     7801 gggcgcgaat cccggccccg agttgccagg ggataggccg cggctgtggc agcgggcagt
     7861 tgcggcgatc cggcgcgtcc tggagtagcc aggcacccgg gccttcggcc aaggcataag
     7921 cggcagcctc tacctgcgac atctcagatc cctcatcgaa cgccgtctcc cacgccgtct
     7981 cgccaagcta ggcacgcgca agttgtatgt tctaccgaga cctttatgac agtgccatcg
     8041 atccgccaag atgttcccgg acaaggaaga atcgctttgg atgctgacag gtacaagaag
     8101 ttgttagcct attgacggaa gctccaagcc aacaccaata ttcgtgcagg atttgtccac
     8161 agagatcccc ttctacaagg tggttttgcc gacggcgatg ctgcgacacc ctattcgccc
     8221 atcatcgccg gttcatcgtt gtccatcagt atgagaacat gaggcgtcaa gcggtgtggt
     8281 gggtagctct cgggggaatc gatctgcgcc tctggggaat gcccggtggt tgggcgagcg
     8341 cgcgttcgag gcgtgaagat agtcgggccg tcgagcacgg gggagccacg acctccgaaa
     8401 gacgaaggag agggcgagtc gagtgatgac gaaggttcga gcgtcggttt gggtactggc
     8461 ggtgctggtg atcgcgggat cggcggcggg atgcgggcag gtttccgatc gggcccagca
     8521 ggaggccgag aagaaggtcg agaacaaagg tcagcaagcc cgggaggagg ccaaggagaa
     8581 gctaaacgcc aagcagcaag aggcgaagga gaagctcgaa gccggccggg aggatctcga
     8641 gaggaaggta gacgacctga agaaggaggt gaacgacctc cagaaggacg tggccacgct
     8701 tcagaagaag atacatgccc aagagcagaa ggaacaggac cagcagataa accagctgaa
     8761 gaaggcgcta aagaagctgc agaagaaggt ggaggcccaa gggcagcaag gccagaagaa
     8821 ttagacgacc gactcgcccg gaagcgcacc cgaggaagtc gtgaaaacga agctggcaga
     8881 attatcgctc acaatgcttt ccgagggcgc cgttactggg cgaagttcgt agggcgtctt
     8941 ccgggcgagt cgggtggata agggtgaggg gatgagcttg tgggagaata ctggccgcga
     9001 cgaggacttc gtgcccataa tagggactac tataatcgta ggcgagcgag aggtggaaga
     9061 tgaacagtaa cgctggcgca agtgccgaag ccctgccctg gtggctggtc ctggttgaag
     9121 gaatagtcgc tgccctcttt gggttgatcc tgttggtggc tccgggagcc tcgctggttt
     9181 ttctggtgtg gctgctgggc atctacctgt tggtagcggg cgtcgtccgc atcgtgggca
     9241 tctttctaga ttcctcctcc tggggctgga agctggtcgc cgggatcctg tgcctcatag
     9301 ccggattggc gatactcagc aacccgcttt ggagcaccac cctggcctcc acgtggctgg
     9361 tcatcctggt ggggttcttg gccatgcttc agggcgctgc gggcctggta gtagctttcc
     9421 aagggggcgg ttggagcatg ggggccctgt ccgtgctagg catcctcttg ggcctcttcc
     9481 tggtgatcaa ccccctgatc ggggctgcgg cgctgacgct aatcctgtct atcttcatgc
     9541 tcatcggggg tgtgggagcg gtggttcagg cgttcaggat gcgcagtgga tcggcgacag
     9601 ttcagcggcc cccgcgcgtg cgctagggca agcgctttcc cagaacgccg tcaaagcgaa
     9661 gtacagcggt tttgcagaga gattgatctt cgtcgaagag ggctatcaga ggtcgctttc
     9721 ttgtccttgg ctgatggctc agagctaacc gctgcgtgct tgcacactgt ctcatttccg
     9781 tcgcaatccg ctgtggacta ctgccagagc ccatagagca ttgacaaggg ttcatcggac
     9841 cactctcgag tggattacct gcgtcgcaga ttgctctggg cgaagttcgg cagatttctt
     9901 ttcgaacgag ggcggggcac caagatgctc gcgcgaaagg gggttcttcc cgtgtcgatc
     9961 gaagagatca aggagatcgc gctcagcctt atcgaagcgc acgacgcccg cgaccaggtg
    10021 tagggcgacc gtgtcggggg tcatgataac caagatacga gacggcaagg ccgtgcgtgg
    10081 ttggttctac tgggatcagc tcgcccgcct ggtccagctg ggcatcaccg aacaacccgg
    10141 gcacttctta tcggcagcgg gcttctagga cagtcaggtt tgcctctggt atccgcggta
    10201 gacggcctcg ttgatctctg ctcctagcag cacgatccac gccgagatgt aaaggaagac
    10261 gagcaaaccc acggccgtcc cgaggctgcc gtaggtcagg ccgcgacccg cgaagtcgga
    10321 caggtagata gagaatccca cagaagcgat aggccacgcg atcacggcga agacggcgcc
    10381 gggcgtcgca gaccagaatg atcgatcggc gtcggggacg aagcggtaga cgaccgccag
    10441 caccgccgag agcaacaaga gcgccacggg gaagcgcagc cagccccaga aagccaccca
    10501 cgcttcgtcc agatccgcga ctcccgccac ccacccggcc acgcgcggtc cgatcagcat
    10561 gagcccacac gcagcgataa aggcgagcgc gaggaggggg ccgaagacaa gggcgaaggc
    10621 gaacctcttg cgccgcgagc gcgtctcctc gacgccgtaa gcggcgttca gagcctccat
    10681 cagggtgaag atcaccgcgg ataccgacca caacgatatg acgaggccga aggagagcaa
    10741 gcctcccccc gcctgctccc gcgcctgctg gatcaacggc agcaggaacg ccgcctgatt
    10801 cctacccttc tcggccatgg gctctatcgg ttccgggatc tcctgcggcg gcacgtccat
    10861 cgccgcttct atgagccggt cgaaaaccgc gtcgatcctg aggacggcca gcagcgcgaa
    10921 gaccaggatg ataaagggga agaacccgaa gaggccccgg taggaaagct gctccgcgta
    10981 gatcgccata cgatgttcga agaagcttct taccgagtgc ctgcccagct caacaacccc
    11041 acgcactaac gtcgctcccg tcctaatgac gctcgctggc gacctctacc ataccttctt
    11101 ccgccctgga ccgactacgc gcatttctcg tgcccaactg gtggtggaaa ttcgccgacg
    11161 cctgttgcac gttcatgagg aactacctgc tccaataggc ggccgcttat tgcctgctcc
    11221 gaagtagcac gagcagccgc agaaccgaaa cgtagatcca gatcagcgat agcatcagcc
    11281 cgaacgcgcc gtaccattgc atgaacctcg gcgcccccgc ctccgccgac ctctcgatga
    11341 aagcaaaatc gagcgtgagg ttcagcgctc caaggatgac gatcaggacg cttataccga
    11401 tgcccaacgg ggtgggagta tacaggaagc ggaaatccac cccgaacaaa gagagcagcc
    11461 acaccaccag gtagagcagc acgaaaccgc ccgtcgccgc catgaccccc gtcgcgaacc
    11521 gcgaggtgac tttgacgatg cggaggctgt agaggaggta cacagccacg aatatgcaca
    11581 acgtggcgag tagcgcctga gagacgatcc cgtcgaacct caggtcgtag tatcgggagg
    11641 cgatgccaag cagcgaaccc tcgccaaggg cgtacaggac ggaggtgatg ggtgcggccc
    11701 tgtaggcgaa agcgcccacg atggcgacga cgaaggttaa aaggaaggcg agccaggtcc
    11761 aggctggggt caaagcctcc ttgcgtccgc ccacgctgac gatctccacc tgcgaccagc
    11821 cgaaagccgc ggcggccacc acgatcacga agagcaaccc gacgatggcg tacgtgcccg
    11881 aggcgctcat gccgcggggc tcggaggcgg cctcttcgat gccgcgccga aaggagcgtt
    11941 cgttgagcgc cggattttcg aggcccatcg ttcctcctgc ttagcacgtc agttgccaac
    12001 accttctgcg atcctatccg agcttagg
//
LOCUS       DBNGVD010000012         3667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3463137, whole genome shotgun sequence.
ACCESSION   DBNGVD010000012 DBNGVD010000000
VERSION     DBNGVD010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3463137"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC16CM_00320"
     CDS             complement(<1..542)
                     /locus_tag="AC16CM_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138299.1"
                     /translation="MSADEERKRGNGVGFVLVVWAVSRLLYLVCGSIFARVVPTSDFQ
                     RVTLDVPFGSMNLWSHWDGEHYAMLALGGYLSPPDNVSPAFFPLYPLLMRSSAELFGG
                     PISAETLSVWGPLLSLLVLPFALYFVYGIALDGWGDRVARGAVLALAFFPTTFFLNAA
                     YTESLFLALSAGSVWAVRVR"
     gene            complement(642..2708)
                     /locus_tag="AC16CM_00325"
     CDS             complement(642..2708)
                     /locus_tag="AC16CM_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138300.1"
                     /translation="MIGDLLQILPAVLVIGVVPGWFWTRILLRSDDLAERLAYTLGLS
                     ITLVPTAALLQTYLFATGVTLTVTLVAAALVFFAGLAVYLLSGPTRKDGEPVCSPPAP
                     PGVLTLVPLVAALALALAMLLGLAPGVWLMIPIAFLVLAAGAIYVWVPGGSDRVSVPE
                     DGRPTPPAARYAVLGVVSLLVLARGYLGPIVNGWPFPRGIDRYEHAVMAGMMMRNGSN
                     ESFMLYPPGFHLLTAMISRLSGFKPMEVFPILAPTLPLLCALACYALARRMWGWEYGV
                     VAALASGLLLGSTYLQFEEARLPNFIGEYFLIVLAVAALIGLYASPGVRGGMLLALLG
                     SSTVLYHQIAGYTLAVLLGVISVSFLPYLLLRDRRRGVYLLLSLAVLGLLSVFYAWDT
                     YDLPSLAAGMLGSSETGRGGEAVAMAIGTKPANGPGHFLMTLSHPILWLGLLGLFMLA
                     GHENTRGTPDTLARLTLFVWALLLFFGSLTSYSGFPDRFERDLGVPLALLAALALVTL
                     LRVPPALGPAGRGALATVVLAVALSATLVGAQSVQSLQLAAGPSTRPKDRPAPADVVA
                     AGTWLGEHNNGGNIVSTPYLDYVPSRGMLAMGGYTRMQSYDAARIERARDLPPFGAGP
                     MWDALHILNNPTGERTARLIEENDVRYIVFYRGSLDADWRLYALHENLYRTVFQNESV
                     IIFAPR"
     gene            2895..>3667
                     /locus_tag="AC16CM_00330"
     CDS             2895..>3667
                     /locus_tag="AC16CM_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3138301.1"
                     /translation="MKAADHTKRTLIIGMHALLALAVACVLSGCFPSAEGKQPEAKAD
                     KPKPPNVVFILTDDLASSDLNENTLEHMPNLKKLLIDKGTTFDESFVSNSLCCPSRAT
                     ILRGQYTHNHQILSNEPPLGGFEKFRYLGHENSTIATWAKEQGYRTAFFGKYLNGYSD
                     SYIPPGWDEWYAVTGNYLSHTFNENGRMVDYDQETDYDTDVMSEKASDYISRTAGADP
                     PFFTADRPFLMWIGTKAPHQPATPAPRHQHAYPDVSLPRP"
BASE COUNT          747 a         1178 c         1231 g          511 t
ORIGIN      
        1 ttccttaccc tgacggccca tacggagccc gcggagagcg cgaggaagag gctctcggtg
       61 tatgcggcgt tcagaaagaa ggtggtgggg aagaaggcga gggcgagcac ggcgcccctg
      121 gccacccgat ctccccagcc gtcgagggct atcccgtaga caaagtagag ggcgaagggc
      181 aggacgagca gggagagcag gggtccccag acagagaggg tctccgcgga tatgggtccg
      241 ccgaacagct cggcggagga tctcatgagc agcgggtaca gcgggaagaa ggccggggaa
      301 acgttgtccg ggggtgaaag atacccgccg agggccagca tggcgtagtg ctccccatcc
      361 cagtgagacc agaggttcat gctcccgaag ggcacgtcca gggtcacccg ctggaagtcg
      421 gaggtgggca cgacccgcgc gaagatagaa ccgcagacga ggtagaggag gcggctcacc
      481 gcccagacca cgagcacgaa acctaccccg ttgcccctct ttctctcctc atcggcactc
      541 atcgccgggg cattatatta gctgcgagct ttcagcccct ggccaccggc cagaaagctg
      601 actgctgaga gctgacagcg cggttcgcgc agcgaaccgc cctagcgggg ggcgaagatg
      661 atgacgctct cgttctggaa caccgttctg tagaggttct cgtgtagcgc gtagaggcgc
      721 cagtctgcgt ctagggaacc cctgtagaac acgatgtagc ggacgtcgtt ctcttcgatg
      781 aggcgcgcgg tacgttcgcc ggtcgggttg ttgagtatgt gaagggcgtc ccacatcggg
      841 ccggccccga agggcggcag gtctctggcg cgctctatcc gcgcggcgtc gtaggactgc
      901 atcctggtgt aaccgcccat agcgagcatg ccgcggctcg gcacgtagtc caggtagggg
      961 gtggagacga tgttcccgcc gttgttgtgc tccccgagcc acgtcccggc ggcgaccacg
     1021 tcggcggggg ccggcctgtc tttggggcgc gtgcttggcc cggcggccag ctgcaggctc
     1081 tggaccgact gtgccccgac gagggtcgcg gagagggcca cggcgagcac gaccgtggcc
     1141 agggccccac gcccggctgg cccgagcgcg ggcggcaccc gcagcaacgt gaccagggcc
     1201 agggccgcga gcagcgcaag gggcacgccg aggtcgcgct cgaagcggtc ggggaagccg
     1261 ctgtaggagg tcaggctgcc gaagaacagc aggagggccc atacgaacag ggtgaggcgc
     1321 gccagcgtat ctggtgtgcc cctcgtgttc tcgtggccgg cgagcatgaa aaggccaagc
     1381 aggccgagcc agaggatggg gtgcgagagg gtcatcagga agtggccagg gccgttcgcg
     1441 ggcttggtgc ctatcgccat cgcaacggcc tcgccgcccc tccccgtctc cgaagagccg
     1501 agcatccccg ccgccaggct gggcaggtcg taggtgtccc aggcgtagaa caccgagagg
     1561 aggcccagaa cggcgagcga gagcaggagg tagacgcccc tcctgcggtc ccgcaacagc
     1621 aggtagggca ggaaagagac cgagatcacg ccgagtagca cggccaaggt gtagcccgcg
     1681 atctggtggt agagcaccgt cgaggagcca agcagggcaa gaagcatacc gccgcgtacc
     1741 ccgggcgagg cgtacagccc gatcagggcg gccacggcga gcactatgag gaagtactcc
     1801 ccgataaagt tcggcaaccg cgcctcctcg aactgcaggt aggtgctacc gaggagcagc
     1861 cccgaggcga gggccgccac caccccgtac tcccaccccc acatgcgcct cgccagcgcg
     1921 taacaggcga gcgcgcacag aagcgggagc gtgggggcga ggatggggaa cacctccatc
     1981 ggcttgaagc cgctgaggcg cgagatcatg gccgtgagca ggtggaagcc gggcgggtag
     2041 agcatgaacg actcgttcga cccgtttctc atcatcatgc cggccatcac cgcgtgctcg
     2101 tagcggtcta tgcccctcgg gaagggccag ccgttcacga tgggcccaag gtatccgcgc
     2161 gcgagcacga ggagagagac cacaccgagt accgcgtagc gtgccgcggg aggggtgggt
     2221 cttccgtcct caggtaccga cacccggtcg cttccgcccg gcacccacac gtagatggcc
     2281 ccagcggcga gcaccaggaa ggctatcggg atcatgagcc agacgccagg cgccaggccg
     2341 aggagcatcg ccagcgccag cgccagggcc gcgaccaggg gcacgagcgt caagacgccg
     2401 gggggcgcag gcgggctgca gaccggctcc ccgtcctttc tggtcggtcc cgacagcagg
     2461 tagacggcca accccgcgaa aaacaccagc gccgccgcaa ccagggtcac ggtcagggtg
     2521 acgcccgtcg cgaacaggta ggtctgcagc agggcggcgg tcggaaccag cgttatggag
     2581 agcccgaggg tgtaggctag ccgttctgcg aggtcgtcag aacgaagcag gatcctggtc
     2641 cagaaccaac cgggaacaac ccctattacc aggaccgcgg ggaggatctg gagcaggtcg
     2701 ccgatcaaac gccaacccgc aacgagggtg ctctggaagt ggacttggaa gtggactcgt
     2761 tcatcgcggg aatgtataac acttaacctg ggtgaaaccc agaggttggt gggctggctt
     2821 gtactaaccg cttctagggt aaaaacagag atactggaac ggctctcctc cgagaaccct
     2881 ccggcgagac caacgtgaaa gcagcggacc acacgaagag aacgctcatc atagggatgc
     2941 acgcgctcct ggcgctggcc gtggcctgcg ttctctcggg ctgtttcccg tccgccgagg
     3001 gcaagcaacc agaagcgaag gccgacaagc ccaagccgcc gaacgtggtc ttcatcctca
     3061 ccgacgacct tgcctcgagc gatctgaacg agaacacgct agagcacatg cccaacctga
     3121 agaagctgct catcgacaag ggcaccacgt tcgacgagtc cttcgtctcc aactccctgt
     3181 gttgccccag cagggccacc atcctgcgcg gccagtacac ccacaaccac cagatcctct
     3241 ccaacgagcc gccgctcggg ggtttcgaga agttccgcta cctgggccac gagaactcca
     3301 cgatcgccac ctgggccaag gagcagggct acaggacggc cttcttcggc aagtacttga
     3361 acgggtacag cgactcctat atcccgcctg gctgggatga gtggtacgcg gtgacgggta
     3421 actacctcag ccacaccttc aacgagaacg gccgcatggt cgactacgat caggagaccg
     3481 actacgacac cgacgtgatg tcggagaagg cgtccgacta catctcgcgc acggcgggcg
     3541 ccgacccgcc gttctttacg gccgacaggc ccttcctgat gtggatcggc accaaagccc
     3601 cccaccaacc tgctaccccc gccccacgcc accagcacgc ctacccggac gtctcactgc
     3661 cacggcc
//
LOCUS       DBNGVD010000013         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4556441, whole genome shotgun sequence.
ACCESSION   DBNGVD010000013 DBNGVD010000000
VERSION     DBNGVD010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4556441"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2114)
                     /locus_tag="AC16CM_00335"
     CDS             complement(120..2114)
                     /locus_tag="AC16CM_00335"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HIR3138302.1"
                     /translation="MRKSIPMFATTACATTLALILLIACITMGAGPARAANTTYSVEN
                     LGALEEDGASIARGVNTSGQVVGQSQERLPNVTPLQLRAFFWDGGPMQDLGTLSGGLT
                     SAGRGINDSGQMVGFSRPSGTTNQQAFVTRRGTDGTTQLEPLGALPGLPSSEAFAINE
                     SGKIVGRSSTLPSSGPVRVGRAFLYQDGAMSDLGTLPGASYSEAWAINDRGEVVGESG
                     SDEDHGEAFLYSDGMKALGTLQGYPYSEAFGINDSGRIVGWSYSSRAAVQGRAFLYKD
                     GTMRDLGTLPGDTYSMARAVDEQGRVVGQSRDASGQNRAFLWEDGAMVDLNSLIPPDS
                     PLKLLDAYAISESGQIAGSAFNEKRQVRAFLLTPNDLTPPNTEATLSPEPNIDGWNSN
                     DVTVSLDATDEGGSNVEGIVYSASGAQTVEETTVGGSSTSLDITAEGETTITYAARDH
                     AGNLEDQKTISVRIDRSDPEVSISSPADGAEYALDESVAADYSCSDALSGLAYCLGTV
                     PDNVGIDTASVGQKTFAVEAADDVGNVVSASSIYHVIYDFDGFFSPVDNPDVLNRVRA
                     GSAIPLKFSLGGDQGLDIFAEAGGSGSPSSRPIPCHPTGPTDTLEQTISANPSRLTYD
                     AATDLYTYVWKTRKDWTGCRQLVLRLDDGKEYTADFEFFE"
     gene            complement(2324..2539)
                     /locus_tag="AC16CM_00340"
     CDS             complement(2324..2539)
                     /locus_tag="AC16CM_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138303.1"
                     /translation="MKSEEACVGKRVRVREDHRKADLRGRRGTIVERWGNPSYAALDV
                     LLDEGEWQLFWYHELEELDEEHSSSLQ"
     gene            complement(2790..3833)
                     /locus_tag="AC16CM_00345"
     CDS             complement(2790..3833)
                     /locus_tag="AC16CM_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3138304.1"
                     /translation="MELGLYTFADVGPEIDPARRLRNLLEEAELADQLGLDVFGVGEH
                     HRPDFAVSSPAVALAAVAARTERIRLTSAVTVLSSDDPVRVFQDFATLDLISDGRAEI
                     MAGRGSFIESFPLFGYDLDDYDDLFSEKLELLAELRDSERVTWSGKHRPSLEDVGVYP
                     RPLQEPLPIWIAVGGTPSSVVRAGTLGLPLAVAIIGGKPERFAPLVGIYREAARRAGH
                     DPARLPVGINSHTYVAESSRRAGDEYYPTYAAMMNRIGRERGWPPIGRGQFEMGRSPR
                     GALAVGSPEEVAEKILHQHEYFGHDRFMAQISVGTLPHEKAMRAIELFGTEVAPVVRQ
                     EIARRTAPEASHQ"
BASE COUNT          665 a         1271 c         1283 g          795 t
ORIGIN      
        1 caactcgtgc tgaggctcga cgacggcaag gagtacacgg cagacttcga gttcttcgag
       61 tagaaaggta aagcgaaggg gccggagtga agcatctccg gccccttcgc tttacctttt
      121 tactcgaaga actcgaagtc tgccgtgtac tccttgccgt cgtcgagcct cagcacgagt
      181 tgcctgcatc cggtccagtc cttccgcgtc ttccagacgt aggtgtagag gtccgtggca
      241 gcgtcgtatg tgagcctgct gggattcgcg gaaatcgtct gctccagggt gtccgtgggg
      301 cctgttgggt ggcagggtat cggcctacta ctcggggagc ccgacccgcc agcctcggcg
      361 aagatatcca ggccctgatc cccaccaagc gagaacttca agggtatggc gctgcccgct
      421 ctgacccggt tcagaacgtc gggattatcg acggggctga agaagccgtc gaagtcgtag
      481 atcacgtggt agatgctgct cgcagagacg acgtttccga cgtcatccgc cgcctcgacg
      541 gcgaaggtct tctgtcccac ggaggcggtg tctatgccaa cgttatctgg caccgtacca
      601 aggcagtacg ccagccccga gagggcatcg gagcaggaat agtcggccgc gacggactca
      661 tcgagagcgt actcggctcc gtcggcgggg gaggaaatag agacctcggg gtcggacctg
      721 tcgatcctga cggagatggt cttctgatct tcgaggttgc ccgcgtggtc ccttgcggcg
      781 taggttatgg tcgtctcgcc ttcggcggtg atgtccaggc tcgtagagct gccgccaacg
      841 gtcgtctctt cgacagtctg ggcaccgctc gcggagtaga cgatcccctc tacattcgag
      901 ccgccttcgt ctgtggcatc gagactgacg gtaacgtcgt tgctgttcca cccgtctata
      961 ttgggttcgg gtgagagcgt ggcctctgtg ttcggagggg tgaggtcgtt cggggtgagc
     1021 agaaaggccc tgacctgacg tttttcgttg aacgcggatc ccgctatctg cccggattcg
     1081 cttatcgcgt aggcgtcgag cagcttcagc ggggaatcgg gggggatcag ggagttgaga
     1141 tcaaccatcg cgccgtcctc ccacaggaag gcgcggttct ggccgcttgc gtcgcgcgat
     1201 tgcccgacga cgcgtccctg ctcgtccacg gcacgcgcca tgctgtaggt gtctcccggc
     1261 agagttccaa ggtctctcat cgtcccgtct ttgtagagga aggcccgtcc ctggacggcg
     1321 gccctggagc tgtacgacca tccgactatt ctgcccgagt cgtttatccc gaacgcctcg
     1381 ctgtacggat acccctggag ggttccgagg gccttcatgc cgtcagagta gaggaaggcc
     1441 tcgccatggt cctcgtcgct acccgactca ccaaccacct cgccgcgatc gttgatggcc
     1501 cacgcctcgc tgtaggaggc tcccggcagg gttccgaggt cgctcatcgc accatcctgg
     1561 tagagaaatg cccggccgac cctgaccggc cccgaactgg gcagcgtgct tgatctcccg
     1621 acgatctttc ccgactcgtt gatggcgaag gcctcacttg agggtaaccc gggcaaggca
     1681 ccgaggggct ccaactgcgt cgtgccgtcc gtgcccctcc tggtcacgaa agcctgctgg
     1741 tttgtggtac cggagggccg tgagaacccg accatctggc ccgagtcgtt tatgccgcgc
     1801 cccgcgctgg taaggccgcc agagagcgtg ccgagatctt gcatcggccc gccatcccag
     1861 aagaaggcac gcaattgcag gggggtgacg ttgggcagcc gctcttgtga ttgcccgacg
     1921 acctggccgg aggtgttgac gcccctggcg atgctcgccc catcttcttc gagcgcgccg
     1981 aggttctcaa ccgagtaggt cgtgtttgcc gcacgggcgg ggcctgctcc catggtgatg
     2041 caggcgatca ggagtatcag ggccagagtt gtagcacacg ccgtcgtcgc gaacatgggt
     2101 atcgactttc tcacagctgc cttcctttcc cgggctgact cacgcgctac gagaacagcc
     2161 tagctggcag ggaggcaagg acgcatcgac cgaatggtcc gttctcgtaa ccgatcggcc
     2221 agtcttccgc gcgcttctca cctaccccgc cgtcgagacg aatggcatgc ccgaacatgc
     2281 actggtacgg ggagatcgac ggtactttcg aggagggtca gttctactgc agactgctgg
     2341 agtgttcttc gtcgagctct tccaactcgt ggtaccagaa cagttgccac tcgccctcat
     2401 cgagcaggac atcaagggcg gcgtagctgg ggtttcccca tctctcgacg atcgtgcccc
     2461 tccggccccg caggtcggcc tttctgtggt cctccctgac ccgcaccctc ttcccgacgc
     2521 aagcttcttc ggatttcatg ggtatcgtgc gcctcttgct cccttccgtc ggcccggaag
     2581 ccaacgctcc ggataacatt cctgcgaacg tggggaatgc cattctcatt gccaggctcc
     2641 tttcctcgta gggcgtgcta cgagacaaat ctagcaacca gacgagcgcc ggggcatcga
     2701 ccagatggtc aatcgtcgtg accgatcggc cattgcggga ccatggtcgg ccgtcgggga
     2761 aagtggtgta tgctccccga tcggcccgac tactggtgcg acgcctcagg ggccgttcgg
     2821 cgggcgatct cctggcggac gaccggggct acctcggtac cgaacagctc gatggcgcgc
     2881 atcgccttct cgtggggcag ggtgccgacg ctgatctggg ccatgaaccg atcgtggccg
     2941 aagtactcgt gctggtggag gatcttctcg gcgacctcct cggggctgcc gacggcgagc
     3001 gccccgcgcg gcgagcgtcc catctcgaac tgaccccggc ctatcggggg ccacccgcgc
     3061 tcgcggccga tgcggttcat catggcggcg taggtcgggt agtactcgtc gcccgcgcgc
     3121 cgggaagatt ctgccacgta ggtgtgcgag ttgatgccca ccggcagccg cgccgggtcg
     3181 tgtccggccc tacgcgcagc ctcgcggtag atgccgacca gcggcgcgaa gcgctccggc
     3241 ttgccgccta tgatcgccac cgccagcggc aggccgagcg tccctgcgcg gactaccgag
     3301 cttggcgttc ctccgacggc tatccagatg ggcaagggtt cctgcaaggg gcgcggatag
     3361 acaccgacgt cttctaggga cgggcggtgc ttgccggacc acgtgacccg ctctgaatcc
     3421 cgcagctcgg cgagaagctc gagcttctcc gagaagaggt catcgtagtc gtccagatcg
     3481 tagccaaaca gggggaacga ttcgatgaac gatccgcggc cggccatgat ctccgcgcgt
     3541 ccgtcggaga tcaggtcaag cgtggcaaag tcctggaaga cgcgcacggg atcatcggag
     3601 ctcaggaccg tgaccgcgct cgtcagccga atacgctccg tgcgggccgc caccgccgcc
     3661 agcgcgacgg ccggcgagga aaccgcgaaa tccgggcggt ggtgctcccc gacgccgaag
     3721 acatccaggc cgagctggtc ggccagctcg gcctcctcga gcaggttgcg tagccgccgt
     3781 gccgggtcta tctcgggccc tacgtcggca aaggtgtata gacctagttc caacttctgt
     3841 tcctttcgcg cctggtccgc caggagcggc cgcttgttgt gtcaggcaac aggtttcagg
     3901 tttaaggaag ggttcgggcc tgatgtctgt agcctctcct tgtatcgggt tcgtcctcga
     3961 aatcgcctct ctaggctacg tgcttgcgcc gaagaggtcg tcggttttcg cggc
//
LOCUS       DBNGVD010000014         2467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_366283, whole genome shotgun sequence.
ACCESSION   DBNGVD010000014 DBNGVD010000000
VERSION     DBNGVD010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_366283"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..690)
                     /locus_tag="AC16CM_00350"
     CDS             complement(25..690)
                     /locus_tag="AC16CM_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3138305.1"
                     /translation="MPGRSEFLSSIRHRTQAGRYKETRAPDVAWTGEPRQGDPIQDPP
                     ARFIEELEALGGHGKRVGSEEEAREYVLWLAREREAELLIRWDVEELDALGVDGPLGD
                     AGVEVAVWRELADFKEVAGRADIGLSTAAWAVAETGSLVLEGGAGMGRSVTLLPPTYI
                     AVVPVERMLRTVPEAIERYAGADGLPANLCFHTGPSRSGDIEMELFVGMHGPGDVHVI
                     LVG"
     gene            complement(691..2160)
                     /locus_tag="AC16CM_00355"
     CDS             complement(691..2160)
                     /locus_tag="AC16CM_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3138306.1"
                     /translation="METRKPSRERETRDRRDEGISSGVEGFNERARRAVADQPLHDSI
                     ELFTHKSVAGRNAVLDALPEAPELRERAYRIKQETMAHLDTYLEQMADAVEARGGNVF
                     FASDGEDVVRYIGDLARSKGAKVITKSKSMATEEIELNKRLEEDYADLDLEIVETDLG
                     EWIAQLAGDTPSHIVGPILHMNRKRIREVLSRTAGEELPPDVEALGSFARRRLREKFL
                     TADIGITGANFGVAETGTVATVTNEGNARLVTSVPPVHVIVMGIEKLVPRFEDLSVFV
                     RLLARSGTGQELTVYTNFITGPRQAGELDGAEELHLILMDNGRSRLLGTEFEEALYCI
                     RCGACLNVCPVYRQTGGHAYGSTYSGPIGAVITPLLKGHDEAKDLPHASSLCGACWEA
                     CPVGIPLHDLLLKLRNQQVEEGIAGKAQEIAFKGFETTMKRPALYNISSRAGRLAQKP
                     LERDGSIRPLPGPLGAWTGSREMPPLAPKSFRELWNEGI"
     gene            complement(2165..>2467)
                     /locus_tag="AC16CM_00360"
     CDS             complement(2165..>2467)
                     /locus_tag="AC16CM_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3138307.1"
                     /translation="ELGILDEPHELLRNVNGLDLLDWQNEELCCGFGGTFSVKMPDVS
                     TAMADEKIKALDESGADTLISGDSSCLMHLEGRLRRTGHDTRVLHIAQVLDDGKAG"
BASE COUNT          381 a          834 c          784 g          468 t
ORIGIN      
        1 cgctgctgag gcgcgcgacc accctcaacc gaccaggatg acgtggacat ctccgggccc
       61 gtgcatgccg acgaagagct ccatctcgat gtccccggac cggctcggac cggtgtggaa
      121 acacaagttg gccggaaggc catctgcgcc ggcgtacctc tcgatggcct ctggaacggt
      181 gcgtagcatt ctttccacgg gtacgacggc gatataggtc ggcggcagca gcgtgacgct
      241 gcggcccata ccggcccccc cctccagcac gagcgacccc gtctcggcta ctgcccacgc
      301 ggccgtagaa agcccgatat cggcccggcc agccacctcc ttgaagtccg caagctcccg
      361 ccacacggcc acctcgacgc cggcgtcgcc caacggtccg tccaccccga gcgcgtcgag
      421 ctcttcgacg tcccagcgga tgagaagctc agcttccctc tcgcgggcca gccagagcac
      481 gtactcgcgg gcctcctcct cgcttccgac cctcttgccg tggccgccga gggcttctag
      541 ctcttcgatg aagcgggccg gggggtcctg tatcgggtcg ccctggcgcg gctctccggt
      601 ccaggcgacg tcgggggccc tggtctcttt gtagcgtcca gcctgggtgc ggtggcggat
      661 ggaactgagg aactccgacc ttcccggcac ctagataccc tcattccaca actcgcggaa
      721 ggacttcggg gcaagcggcg gcatctcgcg cgatccggtc caggctccca atggcccggg
      781 aagcggtcga atggaaccgt ccctctccag aggtttctgg gccaggcggc ccgcccggga
      841 ggagatgttg tagagggctg gcctcttcat ggtggtctcg aagcctttga aagcgatctc
      901 ctgcgccttg cccgctatcc cctcctcgac ctgctggtta cgtagcttca acagcaggtc
      961 atgaagtggg ataccgacgg gacaggcctc ccaacaagcc ccgcagagcg aggaggcgtg
     1021 gggcaggtct ttcgcctcgt cgtggccctt tagcagtggc gttattacgg cgccgatcgg
     1081 gcccgagtac gtggatccgt aggcgtggcc gcccgtctgc ctgtagaccg ggcagacgtt
     1141 caggcaggcc ccgcaacgga tgcagtacag tgcctcctcg aactccgtgc cgagcagtct
     1201 ggagcggcca ttgtccatga ggatgaggtg caactcctcc gcgccgtcga gctcgcccgc
     1261 ctgacgcggt cccgtgatga agttggtgta gaccgtgagc tcctgtcccg tgccgctgcg
     1321 ggcaaggagc cggacgaaga ccgacaggtc ctcgaagcgc ggaacaagct tctctatgcc
     1381 catcacgata acgtggaccg gtggaacgct ggtgacgagc ctggcgttgc cctcgttggt
     1441 gacggtggcc acggtgcccg tctcggcgac cccgaagttg gcgcccgtga tgccgatatc
     1501 ggcggtcagg aacttctcgc gcaggcgcct gcgggcgaag gagccaaggg cctcgacgtc
     1561 aggcggcagt tcttcacccg cagttcggga gagaacctcc ctgatgcgct tgcgattcat
     1621 gtgcaggatg ggtcccacga tgtgcgacgg cgtgtcgccc gcgagctgcg cgatccactc
     1681 cccgagatcc gtctccacga tctccaggtc gaggtcggcg tagtcctctt ccaaccgctt
     1741 gtttagctcg atctcctcgg tcgccatgct cttggatttg gtaatgacct tcgcgccctt
     1801 gcttcgcgcc aggtccccga tgtagcgaac gacgtcctcc ccgtccgagg cgaagaagac
     1861 gttgccgccg cgtgcctcca cggcgtcggc catctgctcc aggtaggtgt cgaggtgggc
     1921 catcgtctcc tgctttatgc ggtaggctcg ctccctgagc tcgggcgcct cgggcagggc
     1981 atcgagtacg gcgttgcgtc cggcgacgga tttgtgggtg aatagctcga tggagtcgtg
     2041 gagcggctgg tccgcgacgg ccctgcgcgc cctctcgttg aagccctcta ccccgctgct
     2101 gataccctcg tcgcggcggt ctctggtctc gcgctcgcgc gacggcttcc gggtctccaa
     2161 ggtgtcaacc agcctttccg tcgtcgagta cctgcgcgat gtggagcacg cgggtgtcgt
     2221 gtcccgttcg cctcaggcgg ccctccaggt gcatcaggca actggagtcg ccggagatga
     2281 gggtgtcggc cccggactcg tccaaagcct tgatcttctc gtccgccatc gcggtagata
     2341 cgtcgggcat cttcacggag aacgttcccc cgaacccgca acataattct tcgttctgcc
     2401 aatccaggag gtcgaggcca ttcacgttcc gcagaagttc gtgcggctcg tcgagtatgc
     2461 cgagctc
//
LOCUS       DBNGVD010000015         5521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2734702, whole genome shotgun sequence.
ACCESSION   DBNGVD010000015 DBNGVD010000000
VERSION     DBNGVD010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2734702"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..413)
                     /locus_tag="AC16CM_00365"
     CDS             complement(243..413)
                     /locus_tag="AC16CM_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138308.1"
                     /translation="MSIAIVSLLVLAVIAIVAVPIMIRVIGEAGRDHANDDGEEDSPQ
                     NRRDPHSGPGRD"
     gene            complement(502..957)
                     /locus_tag="AC16CM_00370"
     CDS             complement(502..957)
                     /locus_tag="AC16CM_00370"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3138309.1"
                     /translation="MAQRVHESIEVNAPVQDVYEYWSNFENFPSFMSNIEEVTMTGQD
                     TSHWKVKGPLGTSVEFDARTTEMNPNRGIGWNTVDGEVMTSGEVRFEEVTPSRTRVEV
                     TMNYSDPPGGAVGETVANALSNPERNLTEDLGNFARIAESGGIGKSRSS"
     gene            complement(1037..2281)
                     /locus_tag="AC16CM_00375"
     CDS             complement(1037..2281)
                     /locus_tag="AC16CM_00375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3138310.1"
                     /translation="MWKDTRSRRELRNSVGRVLVGVVVLLAVAFGGFWLGRAQSPATL
                     DARDRESVALYAEALDVVRNNYVDQQDIDPTQETYGAIKGMLGTLGDGGHTRFLTPAE
                     RKQNDQSLSGTYVGIGVQLEQEKGEIVVAAPIQDSPAEKAGISADDVLVAVDGKSVRG
                     DDVSEVVEKVKGPVGTEVGLTVLHDGKKQTYDLRRAEIDSPAVTWAMIPDSDVAVVLL
                     SSFSDGSAEQLQDAFQKARAAGAKRFVLDLRNNPGGRLDQAVEMAGYFLEPGSVVYIR
                     KDASGERQEIKVEGDPESTDAPLTVLVNGASASSAEILAGALRDNGRATVIGETTYGT
                     GTVLSEFVLRDGSSILLGVAEWLTPDGDFIRETGIEPDVKVPLRNGDDQITPDSASDL
                     SRGQILDRDPQLRGAYKKLQGQ"
     gene            complement(2601..3641)
                     /locus_tag="AC16CM_00380"
     CDS             complement(2601..3641)
                     /locus_tag="AC16CM_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138311.1"
                     /translation="MTENAQASPETNGYAGGFLLQTRNLKMHFPIRAGLLQRTVGHVK
                     AVDGVDLAVRRGETLGLVGESGCGKSTLARLILRLLEPTEGEVLFDGEDILGYDRKRM
                     LDVRRNMQIVFQDPYASLNPRMTVGNIVSEPLRTHDIGGDRKKRVQELLEVVGLSPEH
                     YNRYPHEFSGGQRQRIGVARAIALNPRLIICDEPVSALDVSIQAQVINLLQDLQQEFD
                     LTYVFIAHDLSVVKHISDRVAVMYLGKIVEIADRDTLYNEPRHPYTASLLSAIPVPDP
                     QKERERQRVVLSGDVPSPANPPQGCSFHTRCPRAQDECTQQEPALETQDKENHQAACF
                     FPVQPGQRIEEA"
     gene            complement(3638..4651)
                     /locus_tag="AC16CM_00385"
     CDS             complement(3638..4651)
                     /locus_tag="AC16CM_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138312.1"
                     /translation="MLEVNNLKTHFKTHDGVVKAVDGVSFSLEAGETLGIVGESGSGK
                     SVTALSIMQLNPQPPTEYPEGEILFEDQDLLQTSDKRMQAIRGNDIAMIFQDPMTSLN
                     PVFTVGNQIREAIRIHQNLSKSEADEKTVQVLRDVGIANPERRVREYPHQFSGGMRQR
                     AMIAMGLSCNPKVLIADEPTTALDVTIQAQILELMVDLQEKYGTAIIMITHDLGVVSQ
                     LADNVMVMYAGRAVEQATTDEVFYDPLMPYSWSLLRSLPRLDTAGDVRLLPIEGTPPS
                     LIFLPDGCNFSPRCPFVKDECHRKDPELEEKKPDHKAACILSVEEIEQNKSRVDEELG
                     VGS"
     gene            complement(4818..5117)
                     /locus_tag="AC16CM_00390"
                     /pseudo
     CDS             complement(4818..5117)
                     /locus_tag="AC16CM_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5142..>5521)
                     /locus_tag="AC16CM_00395"
     CDS             complement(5142..>5521)
                     /locus_tag="AC16CM_00395"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3138313.1"
                     /translation="ALREGEPYLAFGTPGGDQQDQWSLHFFLAHAHFGLNLQEAIDAP
                     NFHTEHFPSSFYPRAAAPGRIMVEDRIGPDVVAGLRARDHDVDVVDGWSLGRISAVSR
                     DTRDGSMRGAANPRGMQGYAVGR"
BASE COUNT          883 a         1744 c         1775 g         1119 t
ORIGIN      
        1 ccaagagact gacctggtga gagagggcta tcggccttca gctatcggta gggaagagcc
       61 ggaagctgaa ggttttcatc gtcaggggct gattacccta caaactgctg cgagtggcgt
      121 acgtgaaacg tcgacgtgga acctgcctct acttccgggc ctgactccag gcttcgactg
      181 cccaaacggg gtcaggatcg tcgagccttc ctgaaacctg tggcctgagg catgcagcct
      241 gcttaatctc ggcctggtcc ggagtgtggg tcgcggcgat tttgtggcga gtcttcctcc
      301 ccgtcgtcgt tcgcgtggtc acgtccggcc tcacctatta cgcggatcat gattgggacg
      361 gcgacgatgg cgattactgc cagcactagc agtgagacta tggctatgga caaggttctt
      421 cctccctgcg agaggttaga ggccaccggg tgtttctacc ccggcggcct ctagattact
      481 ccctgtagag ggtgtggtct gttagctgct ccgggacttg ccgatcccgc cgctttcggc
      541 gatcctggcg aagtttccaa ggtcctcggt aaggttccgc tccgggttgg agagggcgtt
      601 ggcgacggtc tcaccgacgg ccccaccggg cggatcggag tagttcatcg tcacctctac
      661 gcgggtgcgg ctcggcgtaa cctcttcgaa acggacctca ccgctcgtca tgacctcgcc
      721 gtctacggtg ttccagccta taccgcggtt cgggttcatc tcggtggtcc tggcgtcgaa
      781 ctccacgctc gtgccaaggg gcccctttac cttccaatgg ctggtgtcct ggcccgtcat
      841 cgtgacctct tcgatgttgg acatgaagct cgggaagttc tcgaagttgg accagtattc
      901 gtagacgtcc tggaccggtg cgtttacttc gatgctttcg tgtacgcgct gtgccacggc
      961 tttcctctct gttgcttggt agtctagtct tgcccatgag ggtgccagac ccaaacatct
     1021 ttttatgtga agtgcgctac tgaccttgta actttttgta agccccgcgc aactgggggt
     1081 ccctatcgag tatctggccc ctggagaggt cgctggcgct atctggtgtg atctggtcgt
     1141 ctccgttccg tagcggcacc ttgacgtcgg gttctatgcc cgtttcgcgg ataaagtccc
     1201 cgtctggggt cagccactcc gcgacgccga gcaggataga agagccatcc ctgaggacga
     1261 actcggagag cacggtgccg gtgccgtagg tggtctcccc tatcacggtc gcccggccgt
     1321 tgtctcgcag ggcgccggcc aggatctcgg cgctcgacgc cgaggccccg ttgaccagga
     1381 ccgtaagcgg cgcgtccgtg gactccgggt cgccttcgac cttgatctcc tgacgctctc
     1441 ccgaggcgtc tttcctgatg tacacgacgc tgcctggctc cagaaagtac cccgccatct
     1501 cgacggcctg gtcgagcctc cctcccgggt tgttcctcag atccaggaca aagcgtttcg
     1561 ccccagccgc ccgggccttc tggaaggcgt cctgaagttg ctcggcgctg ccgtcggaga
     1621 aagacgagag gagcacgacg gccacgtcgg agtcggggat catggcccac gtgaccgcgg
     1681 gcgagtcgat ctccgcgcgc cgcaggtcgt aggtctgttt ctttccatcg tggaggacgg
     1741 tcaagccgac ctccgtccca acaggaccct tgaccttctc gacgacctca gaaacgtcgt
     1801 cgcctctcac gctcttgcca tccacggcga ccagcacgtc gtcggcgctg ataccagcct
     1861 tctcggccgg agagtcctgt atcggggcgg cgaccacgat ctcgcccttc tcttgctcca
     1921 gctggacccc gatgcccacg tacgttccag agaggctctg atcgttctgc ttgcgctccg
     1981 ccggggtcag gaagcgcgtg tgccctccgt cgcccagcgt cccgagcatg cccttgatcg
     2041 ccccgtaggt ctcctgcgtg gggtcgatgt cctgctggtc cacgtagttg ttcctgacca
     2101 cgtcgagcgc ctccgcatag agtgccacgc tctccctgtc ccgggcgtca agcgtcgccg
     2161 ggctctgcgc ccgccccaac cagaagcccc cgaacgccac cgcgagcaga accacgacgc
     2221 caacgagcac ccgaccaacc gagttccgca gctcacgcct actccgggta tctttccaca
     2281 cgcgacgatt gtatctcagc acgctcgggc taacgcctcc gcttgtcagc acggcgcctg
     2341 cagcgccttg tcggcacgct cggcttcgcc tcgctttcgg ctagtcgtct ctctccctca
     2401 cggcaagcca ggtatgaggc tttcctgcac agcgatattc cctgtgcgaa gggtgcgggc
     2461 cgcagcggga tctagctgac agcgagccag gcgcagccgg gcgatgcgtg ccgacaagcg
     2521 agggcgcaag cccgagcgtg ctgacgagcg caggccgaag gccggagcgt gcgcgcgcgg
     2581 aggcggagcc gtagcgtgct ctaggcttct tctatgcgtt ggcccggctg gacggggaag
     2641 aagcaggctg cctggtgatt ctctttgtcc tgtgtttcga gagcgggttc ctgttgcgtg
     2701 cactcgtcct gggcccgggg gcatcgggtg tggaatgagc agccttgcgg cgggttggcg
     2761 ggagaaggta cgtcgccgga gaggaccaca cgctggcgtt cgcgttcctt ctggggatcc
     2821 gggacgggga tggccgagag aagggaggcg gtgtacgggt ggcggggctc gttgtagagc
     2881 gtgtccctgt cggcgatctc cacgatcttg ccgaggtaca tgaccgcgac gcggtcggag
     2941 atgtgcttca ccacggagag gtcgtgggcg atgaagacgt aggtcaggtc gaactcctgt
     3001 tgcaggtctt gcaggaggtt gatgacctgg gcctggatcg agacgtcgag ggccgagacg
     3061 ggctcgtcgc agattatgag gcgcgggttc agggcgatgg ccctggcgac ccctatcctc
     3121 tggcgctgcc cccccgagaa ctcgtgcggg tagcggttgt agtgctcggg gctcaggccg
     3181 accacctcga gcagctcctg cacgcgcttt ttgcggtcgc cgccgatgtc gtgggttctc
     3241 aggggctcgg agacgatgtt gcccacggtc atgcgggggt tgaggctcgc gtagggatcc
     3301 tggaagacta tctgcatgtt tctgcgtacg tcgagcatgc gcttgcggtc gtagcccagg
     3361 atgtcctcgc cgtcgaagag tacctctccc tccgtcggct ccaggaggcg caggatcagg
     3421 cgggcgagcg tgctcttgcc gcagcccgac tcgccgacga ggccaagcgt ctccccgcgc
     3481 cgcacggcga ggtccacgcc gtccacggcc ttgacgtgtc cgaccgtgcg ctgcaacaaa
     3541 ccggccctaa tagggaagtg catcttcagg tttctggtct ggagcaggaa gcctccggcg
     3601 tagccgttgg tctcggggct ggcctgcgcg ttctcggtca cgagccgacc cccagctcct
     3661 cgtctaccct gctcttgttc tgctcgatct cctcgacgga gaggatgcac gccgccttgt
     3721 ggtccggttt cttctcttcg agctcggggt ccttccggtg gcactcgtcc ttgacgaacg
     3781 ggcagcgggg gctgaagttg catccgtcgg ggagaaagat cagtgacgga ggcgtcccct
     3841 ctatggggag aagccgcacg tcgccggcgg tgtccaggcg aggcagggac ctgaggagcg
     3901 accacgaata gggcatcagg ggatcgtaga agacctcgtc cgtggtggcc tgctccacgg
     3961 cccggccggc gtacatcacc atcacgttgt cggcgagctg cgagacaacg ccgaggtcgt
     4021 gcgtgatcat gatgatggcc gtgccgtact tctcctgcag gtcgaccatc aactccagta
     4081 tctgcgcctg gatcgtcaca tcgagggcgg tcgtcggctc gtccgctatc aggaccttcg
     4141 ggttacagga gagccccatg gcgatcatgg cccgctggcg catgccgccg gagaactggt
     4201 gcgggtactc cctgacccta cgctcggggt tggcgatacc cacgtccctc aagacctgta
     4261 ctgtcttctc atcggcctcg ctcttggaga ggttctggtg gatgcggatg gcctcccgga
     4321 tctggtttcc aacggtgaag accgggttca ggctcgtcat ggggtcctgg aaaatcatgg
     4381 ctatgtcgtt gccccggatg gcctgcatcc gcttgtcaga ggtctggagc aggtcctgat
     4441 cctcgaacag gatctcaccc tccggatact ccgtcggcgg ttgggggttg agctgcatga
     4501 tggagagggc cgtgacgctc ttgccgctcc ccgactcccc gactatcccc agggtctccc
     4561 ccgcctccag cgagaacgag accccgtcga ccgccttcac gaccccatca tgggtcttga
     4621 agtgagtctt caggttgttc acttccagca tagccacagg ctactcctcg ctctccaggt
     4681 ggccgttcca tggaaaagag tacaggagga gaaggctaca ggctacaggc ctcgggcgac
     4741 aggtgttttt gctgatacct gtaacccgat gcctggcgcc ttgacccacc cggattactc
     4801 tctccgaaag catcacgcta gtcgtaggtt ctggggtcga gggcctcgcg gacgccgtcg
     4861 ccgagcaggt tgaaaccgag cacggcgaag aagatggcca taccggggaa gaacgccatc
     4921 caccaggccg tcgataggta ggactgggcc tggaagagca tggaccccca gctcgggtcc
     4981 cccggttcgg caccgagccc gagaaaagag agggtcgcct cggcgatgat cgcgtagcct
     5041 atcgcaaggg tcgtctgcac caggagcggc ggcagtgagt tggggagcac gtggcgcagc
     5101 aggatcatcg tgtttccggt gcccgttgcc tccggatgga gctagcgtcc cacggcgtag
     5161 ccctgcatgc ccctcgggtt ggccgcccct cgcatggacc cgtcccgggt atccctgctt
     5221 acggcggaga tcctgccgag agaccaaccg tctaccacat ccacatcgtg gtctctggcg
     5281 cgcagcccgg ccacgacgtc cggccctatt ctgtcctcga ccatgatcct gccgggcgcc
     5341 gccgcgcgcg ggtagaagga gctcgggaag tgttcggtgt ggaagttcgg cgcgtctatg
     5401 gcctcctgca ggttgagacc gaagtgggca tgagccagga agaaatggag cgaccactgg
     5461 tcctgctggt cgccgccggg ggtaccgaag gccagatagg gctccccctc gcgcaacgcc
     5521 a
//
LOCUS       DBNGVD010000016         6480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1277356, whole genome shotgun sequence.
ACCESSION   DBNGVD010000016 DBNGVD010000000
VERSION     DBNGVD010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1277356"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2957)
                     /locus_tag="AC16CM_00400"
     CDS             complement(<1..2957)
                     /locus_tag="AC16CM_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3138314.1"
                     /translation="MARVESEKLEPEEEHNGADRGVAGPTYARGIPGDDVRWLRSIVE
                     NTMEIVKIVALDGTLLYANPAFERILGYDPRATIGTMNVLDHIHPDDLEHVGKETERA
                     LSEGGTATSRAEYRFRHADGSWRWLESVSIYLTDDPAVGGVVVTVRDVTERKEAEGAL
                     RESEERFRAQSRELALLHGVRSAVAHELDVSGVLSRAVEAIVDTYGYTGTSAYLLRGG
                     ELVLESQAGSHDAIERIPLTEGVCGRAVRTGKPELVEDVGVDPDFIGADRGVTSEICV
                     PLFDGDEAVGCLNVESTGGAGLTRDDLRVMVAVGEHVGVAVSRALLHARVRASERRYR
                     HLTQNSSDLVTLMEASGIIRYQSLALERMLGYSPAELLGKNAFDYVHPDDLDRVRSAY
                     IEGLEDPDARPTVEYRFRHADGSWRWLESVGANLTDEPGVNSYVVNSRDITERREAEE
                     RLRQAEKQYRTLVEQIPAITYMQEMDRYSTSVYVSPQVVDIVGYTPEECTENPNLWIE
                     MLHPEDRERVLAEDERTNQTGEPFVMEYRQFAKDGHLVWIRDEATLVRSEEGSPLYWL
                     GVQVDITERKQAERALAAAEQRYRTLVEQIPAVTYIDPVDDPDTSLYTSPHIERMLGY
                     TPEEWTEGKQWPKRLHPADRERILAADERFEKGDGEPFSEEYRLLARDGSVVWVREEA
                     VLLKDDAGEPLYWQGIIVDVSEQKEAEQQLREAEERYRALVENIPVVAYTQEVGNLKT
                     AIYVSPRIEDLTGYSSEEFAEDYDLWYGVIHPDDRWAVAAEDERTERTGEPFVMEYRM
                     VHREGHVLWVRDQSVLVRDETGKPLYWQGVMSDITERKSLEDQLEYRAFHDHLTDLPN
                     RRLFLDRLHGALDRTRGRTNRQVAVLFMDLDDFKSVNDSLGHEAGDLILNAVAGRMES
                     CLRPEDSLARFGGDEFVVLLEAIRGPEAAVQVAERIARELGKPFVLDGKELFVSSSIG
                     ISVGNAKTKTSEGL"
     gene            complement(3071..3643)
                     /locus_tag="AC16CM_00405"
     CDS             complement(3071..3643)
                     /locus_tag="AC16CM_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138315.1"
                     /translation="MTDSARRAASALTNALNPFVIFTALYALVALSYAGIARGLLFLG
                     VELLSAAGVAGYVLYMRRRSRVGDFWMSARAERLSPALILLAAFVALLAVLFLLQAPA
                     ELFLLALSMGIASAAVALITLLWKASAHCTVAGHAATAGLLLLGPWGLLFLLILPLVI
                     WSRIAMGAHTPLQALVGTAIGAAFVVLTLM"
     gene            3662..4459
                     /locus_tag="AC16CM_00410"
     CDS             3662..4459
                     /locus_tag="AC16CM_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138316.1"
                     /translation="MQRPRTPAPENEALELWYELGRLRREAGGGEQRATKLGLAAVLA
                     TCALVLLSAPVFGTAWAGPFAAAIPVGAGLASGGGLFLRQRSRLRPRVDAVTARLAER
                     GLDAERPARDGLDAYYDAQLVLLRSEYEYLLLRGAGKSAGLLEATFGFTPEDAFETGP
                     LNVVPDTPEMLGLRERWERRIRSRAQHGIESPALGTREESSYRVFPREMTVPAELSVR
                     RAYLEISRRLILERYGPAGRAHIPGAVRQRAERDLREYEALSRELTG"
     gene            complement(4440..5021)
                     /locus_tag="AC16CM_00415"
     CDS             complement(4440..5021)
                     /locus_tag="AC16CM_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138317.1"
                     /translation="MKNDKAPEFDLQRPFTSTISVLRAVLFSPKTFYSNLEVDGPMRE
                     PAVFALVVGAVSGVLGAALALTSNVLFGDFGAGDLRAALVGAVLFAVLSPVGVGVAAA
                     IYLVSIRTFVGKVAGFAEVYRMVAYAASVLVLAWIPVLGAFAFTYALMVLMGLAIQSA
                     YKTSFITTVITVFVGFVPVASLLVWVAVNQLTL"
     gene            5112..5624
                     /locus_tag="AC16CM_00420"
     CDS             5112..5624
                     /locus_tag="AC16CM_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138318.1"
                     /translation="MFKLYLTSILVAEVVVLAVLAMTSGVALYHIAIIPLTLLAAFIL
                     RRIFGGRVRGLRRLSPEAIVDYRSRASRADSPEALSRAIVERAEEIQKSLEESPSDEV
                     RIEMCAIGYRACANDMITLTHLTNEALPNASFIRRFRLRHGRKRAIDSLSEARKALPP
                     GALRPATQEK"
     gene            complement(5650..5892)
                     /locus_tag="AC16CM_00425"
     CDS             complement(5650..5892)
                     /locus_tag="AC16CM_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3138319.1"
                     /translation="MQKQRDMYDRIEKALDQVRPALKADGGDARVVSCDEETGAVSIQ
                     MLGACKGCPLSQLDFVYAIETLIRREVPEVGEIIAV"
     gene            5981..>6480
                     /locus_tag="AC16CM_00430"
     CDS             5981..>6480
                     /locus_tag="AC16CM_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3138320.1"
                     /translation="MVKDEGQNLLVIGAPEHDADAYHLSGFLAPDPVICLRVAGETYL
                     AVSSLEYGRAEKEAPVDRLLSYDELGIMRLARELKSGAKAYAAAVANLLDELGATNSP
                     LLVPAGFGIAYADELRARGITLKPDGKVFDGLRRSKTEQEISHIERTQEAVEAACAHA
                     VGILEEA"
BASE COUNT         1114 a         2188 c         2055 g         1123 t
ORIGIN      
        1 aggccctcgg acgtcttcgt cttggcgttg cccacgctga tgccgatgct ggaggagacg
       61 aacaactcct tgccatccag cacgaacggt tttccgagct ccctcgcgat cctctccgcc
      121 acctgcacgg cagcctcagg gccccgaatg gcttcgagga gaacgacgaa ctcgtcgccc
      181 ccgaatcggg caaggctatc ttccgggcga aggcagctct ccatacgccc ggccacggcg
      241 ttgaggatca ggtcccccgc ctcgtgaccc agggagtcgt tcacggactt gaaatcgtcg
      301 agatccatga acagcactgc cacctgccga ttcgttctgc ccctcgtgcg atccagggcc
      361 ccgtggagcc ggtcgagaaa gagccgtcgg ttcgggaggt cggtgaggtg gtcgtggaag
      421 gccctgtact ccagctggtc ctcgagggac ttgcgttccg tgatatcgga catcacgccc
      481 tgccagtaca gcggttttcc cgtctcgtct ctgacgagaa ccgactggtc gcgcacccac
      541 agcacgtggc cctcgcggtg gaccatacgg tactccatca cgaaaggctc gcccgtcctt
      601 tcggtccgct cgtcttccgc cgccaccgcc caccggtcgt cgggatgtat gaccccgtac
      661 cagagatcgt agtcctcggc gaactcctcg ctactgtagc cggtcaggtc ctctatacgg
      721 gggctgacgt agatcgcggt cttcaggtta ccgacctcct gggtgtaggc caccacaggg
      781 atattctcga ccagcgcgcg gtagcgttcc tccgcctccc ggagttgctg ttcggcctcc
      841 ttctgctcgc tgacgtcgac gattattccc tgccagtaga ggggctctcc cgcgtcgtcc
      901 ttgagcagca ccgcctcttc ccgcacccat accacggacc cgtccctggc cagcagacgg
      961 tactcctcgc tgaagggctc cccgtccccc ttctcgaagc gctcgtcggc ggccagtatc
     1021 ctctcccggt cggcgggatg cagacgcttg ggccactgct taccttctgt ccactcctcg
     1081 ggggtgtaac cgagcatccg ctctatgtgg gggctggtgt agagagacgt gtccggatcg
     1141 tcgaccgggt ctatgtaggt gacggcgggt atctgttcca ccagggtgcg gtagcgttgc
     1201 tccgcggccg ccagcgccct ctcggcctgc ttgcgctcag ttatatccac ctgcacgcca
     1261 agccagtaga ggggcgaacc ctcctcgctc cgcaccagcg tcgcctcgtc cctgatccag
     1321 accaggtgcc cgtccttggc gaactgacgg tattccatga cgaagggttc cccagtctgg
     1381 ttggtcctct cgtcctcggc caggaccctc tccctgtcct cgggatggag catctcgatc
     1441 cagaggttcg ggttctcggt gcattcctcc ggggtgtaac ccacgatgtc tactacctgg
     1501 gggctgacgt acaccgaagt gctgtagcgg tccatctcct gcatgtaggt gatggccggt
     1561 atctgctcca ccagcgtgcg gtactgtttc tcggcctgcc tgagacgctc ttcggcctcc
     1621 ctgcgctccg ttatgtccct ggagttgacc acgtagctgt tcacgccagg ctcgtcggtc
     1681 aggttcgcgc cgacggactc tagccagcgc caggagccgt cagcgtggcg gaagcggtac
     1741 tccaccgtgg gtcttgcgtc agggtcctct aacccctcga tgtaggccga tctcacccgg
     1801 tccagatcgt cggggtgcac gtagtcgaag gcgttcttgc cgagaagctc cgccggggag
     1861 tatccgagca tccgctccag ggcgaggctc tggtaacgga tgatgccgga ggcttccatc
     1921 aacgtgacga ggtcggaaga gttctgggtg aggtgccggt atcgtctctc gctggcgcgt
     1981 accctggcgt ggagcagcgc ccggctcacg gccaccccca cgtgctcgcc caccgccacc
     2041 atcaccctca ggtcgtctcg cgtcaacccc gccccgcccg tgctctccac gttaaggcag
     2101 ccgacggcct catccccgtc gaagagcggg acgcagatct cggaggtcac gcccctgtcg
     2161 gccccgataa agtcggggtc gacgccaacg tcttccacga gctcgggttt cccggtacgg
     2221 acggctcgcc cgcagacgcc ctccgtgagg gggatgcgtt cgatcgcatc gtgggatccg
     2281 gcctggctct ccagcaccag ttccccgccc ctgagcaggt aggcgctcgt accggtgtaa
     2341 ccgtaggtat cgactatggc ctcgaccgcc cgggagagaa cgccggatac atccagctcg
     2401 tgcgccacgg cggagcgaac accgtgcagg agggccaact cccttgactg ggcgcggaat
     2461 cgctcctcgc tttccctgag cgccccttcg gcctccttgc gctcggtaac gtccctcacg
     2521 gttaccacca cgccaccgac ggcggggtcg tcagttagat atatactcac gctctccagc
     2581 cagcgccagg agccgtcggc gtgacggaag cggtactcgg ccctgctcgt ggccgtacct
     2641 ccctccgata gggctctctc cgtctccttg ccaacgtgct ccaggtcgtc cgggtgaatg
     2701 tggtcaagca cgttcatcgt gccgatcgtt gccctgggat cgtagcctag gatacgctcg
     2761 aaagcggggt tggcgtagag cagggtgcca tcgagggcca cgatcttcac gatctccatg
     2821 gtgttctcta cgatcgagcg cagccaccgg acgtcgtccc cggggatccc gcgtgcatac
     2881 gtgggccctg cgacgccccg gtcggcgccg ttgtgctctt cttccggttc tagcttctcg
     2941 gactccaccc ttgccatctc tccgaccatc cgttactggc cagacatcag tatacagaag
     3001 ccgtactacc ccagatcatc cccacgccga cccgggtagc gatgagcttg ccgcggtgcc
     3061 ctccctttgg ctacatcagg gtgagcacca cgaacgcggc cccgatcgcc gtccccacga
     3121 gcgcctgcag cggggtgtgc gcgcccatgg cgatccggga ccagatcacg agcggaagaa
     3181 tcaggaggaa gaggaggccc cacggtccga gaagcaggag ccccgcggtg gcggcgtgcc
     3241 ccgcgaccgt gcaatgggcg ctcgccttcc agaggagcgt gatcagggcc accgccgcgc
     3301 tcgcgatccc catggagagc gccaacagga acagctccgc aggggcctgg agcaggaaca
     3361 ggacggcgag gagcgccacg aaagcggcga gcaggatcag ggcgggagag aggcgttcgg
     3421 cgcgggcgga catccagaaa tcccccaccc gtgagcgccg ccgcatgtaa agcacgtacc
     3481 cggccacgcc cgcggccgag aggagctcaa cacccagaaa gaggagccca cgcgcgatgc
     3541 cagcatagga gagcgccacc agggcgtaga gcgccgtgaa gattacgaaa gggttgagcg
     3601 cgtttgtgag ggcgctggcg gccctccggg cactgtctgt catgcgggta gaatagggat
     3661 cgtgcaacgc ccgcgcacac cagcccccga aaacgaggcc ctggagctgt ggtacgagtt
     3721 ggggcgcctc cgcagggaag cgggaggcgg cgagcagcgg gcaacgaagc tgggcctcgc
     3781 cgccgttctg gccacctgcg cgctggtttt gctctcggcc cccgtattcg gcacggcctg
     3841 ggccggaccc ttcgcggccg cgatacccgt gggggccggg ctggccagcg gcggcggcct
     3901 cttcctccgg caacgctcgc ggcttcggcc ccgcgtggac gcggtcacgg cgcggctcgc
     3961 ggagcgtggg ctcgatgccg aacgcccggc gcgagacggt ctcgacgcct actacgacgc
     4021 gcaactcgtc ttgctgcgaa gcgagtacga gtacctcttg ctgcggggcg cgggcaagag
     4081 cgccgggctc ctggaagcga cgttcggctt cacccccgag gacgcgttcg agacgggccc
     4141 cctaaacgtg gtcccggaca cgcctgagat gctggggctg cgcgagaggt gggagcgcag
     4201 gatccgctcc agagcgcagc acgggatcga gtcacccgcc ctgggtacgc gcgaggagag
     4261 ctcataccgc gtgttcccca gggagatgac cgtgcctgcc gagctctcgg tgcggcgggc
     4321 gtacctggag atctcgcgtc gcctcatcct ggaacgctat ggaccggcgg gccgggcaca
     4381 cataccggga gctgtccgac agagggcgga gcgggacctc cgcgagtacg aggccctctc
     4441 tagagagtta actggttgac ggcgacccag accagtagcg aggcgacggg cacgaaaccg
     4501 acgaagaccg tgatgacggt cgtgatgaaa gaagtcttgt aggcgctctg gatggccaac
     4561 cccatcaaca ccatgagggc gtaggtgaac gcgaaggccc cgagtactgg tatccacgcg
     4621 aggaccagga cggaggcggc gtaggcaacc atgcggtaga cctctgcgaa acccgccacc
     4681 ttcccgacga acgtcctgat cgagaccagg tagatcgccg ccgccacacc taccccgacc
     4741 ggcgagagca cggcgaagag cacggcgccg accagcgccg cccgcagatc gccggccccg
     4801 aagtcgccga agagcacgtt ggaagtcagg gcgagggcag caccgaggac gcccgagacc
     4861 gcgccgacca ccaacgcgaa gacggcaggc tccctcatgg gaccgtcgac ttcgaggttc
     4921 gagtagaacg tcttcgggga gaaaagcacc gcccttagca cggagatggt gctcgtgaag
     4981 gggcgttgca gatcgaattc tggggcttta tcgttcttca cgagcgagat tatacagtcc
     5041 gtcgggttcg cgcttgcgaa atctctctcc gaaacataga ctgaaggccc tcgaagacgg
     5101 tggcggagag gatgttcaag ctctacctaa cctcgatact ggtagccgag gtagtggtcc
     5161 tcgcggtgct cgctatgacg tcgggcgtgg ccctgtacca catcgccata atcccgctga
     5221 cgctgctcgc ggccttcatc ctgcgccgaa tattcggtgg ccgagtccgc ggcctgcgca
     5281 ggctctcccc cgaggcgatc gtggattaca ggtcacgagc ctcccgcgcg gactccccgg
     5341 aggcgctctc ccgcgccatc gtggagcgtg ccgaggagat ccagaagtcg ctcgaggagt
     5401 ccccctcgga cgaggtccga atcgagatgt gcgccatcgg ctacagggcc tgcgcgaacg
     5461 acatgatcac cctgacacac ctgaccaacg aagccctccc caacgcctcc ttcatccgac
     5521 gtttcaggct ccgtcacggc cgcaaaaggg cgatagactc cctctcggaa gccagaaaag
     5581 ctctcccgcc cggcgccctg cgccccgcaa cgcaagaaaa gtagcacgtt ccggcgtagc
     5641 cggagcgtgc tagacagcga ttatttcgcc gacttcgggg acttcgcggc ggataagggt
     5701 ttctatggcg tagacgaagt ccagttggga gagcgggcat cctttacagg cacccagcat
     5761 ctggatgctg acggcaccgg tctcctcgtc gcaagacacc accctcgcgt ctccgccgtc
     5821 ggccttcagc gccgggcgga cctggtcgag ggccttctct atccggtcgt acatgtcgcg
     5881 ttgcttctgc atctatggcc gattatatct gttcgcgggg ctgtatttcg cccgtagtag
     5941 aatggggcgc tagagcgatg ctgtcacggg aaggtgtttt atggtcaagg acgagggtca
     6001 gaacctgctg gtcatcgggg cgcccgagca cgacgccgac gcctatcatc tctcggggtt
     6061 cctcgcgcca gacccggtga tctgcctgcg cgtcgcggga gagacctatc tggccgtctc
     6121 gtccctggag tacgggcggg ccgagaagga ggcccccgtg gacaggctcc tctcctacga
     6181 cgagctgggg atcatgcgcc tggcccgcga gctcaagagc ggcgcgaaag cgtacgccgc
     6241 cgccgtggcc aacttgctcg acgagctggg cgccacgaac tcccccctcc tcgtaccggc
     6301 tggcttcggc atcgcgtacg ccgacgagct gcgggcccgc ggcatcaccc tcaagcccga
     6361 cggcaaggtc ttcgacggtc ttcggcgctc caaaacggaa caggagatct cccacatcga
     6421 gagaacccag gaggccgtgg aggcggcctg cgcccacgcc gtcgggatac tggaagaggc
//
LOCUS       DBNGVD010000017         4698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2311, whole genome shotgun sequence.
ACCESSION   DBNGVD010000017 DBNGVD010000000
VERSION     DBNGVD010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2311"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16CM_00435"
     CDS             <1..275
                     /locus_tag="AC16CM_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3138321.1"
                     /translation="SIITTDDAYSEDPRKIAGEVEAGADPSRTCVELDRQAAIRRALQ
                     EAREGDVVVVAGKGHETVQHLPEGDVPFHDATVVGELLAEMGERGR"
     gene            284..544
                     /locus_tag="AC16CM_00440"
     CDS             284..544
                     /locus_tag="AC16CM_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR3138322.1"
                     /translation="MQRQTRSEVVRRLKSVEGHVRGVQKMVEEGDVDYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAEKDGASDAMINDLIKAIDRVTK"
     gene            549..797
                     /locus_tag="AC16CM_00445"
     CDS             549..797
                     /locus_tag="AC16CM_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138323.1"
                     /translation="MSRVRPETLLFVLLLTVPLVVGVAGVAAFLLMRAGFGFLIWGAV
                     PFVASLLAVAGLSFVLSRAARASRDQSPEEPRRGRDGV"
     gene            808..1098
                     /locus_tag="AC16CM_00450"
     CDS             808..1098
                     /locus_tag="AC16CM_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138324.1"
                     /translation="MEDTGREQEDLEAKVGELEKLTDSLNEVSDEELVGALGEAVELL
                     SDINARIEDRLDAAGEESREIGDLLGETDFGPFDRALEEHEAKERTSGEPGV"
     gene            1082..1615
                     /gene="pyrE"
                     /locus_tag="AC16CM_00455"
     CDS             1082..1615
                     /gene="pyrE"
                     /locus_tag="AC16CM_00455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006180106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3138325.1"
                     /translation="MSLVSDLAPRIREAALLEGDFVLSSGERSSFYVDKYLFSTEPDL
                     LRDVAGALAEQMPDGVERLAGVELGAVPLVVATSLATGLPYVIVRKSAKEYGSSAGEN
                     IEGRLNAGETVVLVEDVVTTGTQAVRAAGHLRDAGVEVVSIVAVLDRREEDGGEIGGF
                     PFRALLRMDELRVVKRD"
     gene            1693..1971
                     /locus_tag="AC16CM_00460"
     CDS             1693..1971
                     /locus_tag="AC16CM_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3138326.1"
                     /translation="MALNKTALIEEIASKANSSKSDAQKFFDAFTEVVTDSLKSGEQV
                     QITGFGKFYVQEREARQGINPQTKQRINIAASKVPKFTAGNALKDQIK"
     gene            2054..2893
                     /gene="pyrF"
                     /locus_tag="AC16CM_00465"
     CDS             2054..2893
                     /gene="pyrF"
                     /locus_tag="AC16CM_00465"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3138327.1"
                     /translation="MQALVEAARARDSVLVAGIDPVPARFPPGLRGLPEADATLRFCT
                     GILEAVEPYAAAVKLQAAFFERLGPAGMEVYRALIAASEELALPVIADVKRGDIGPVA
                     TAYAEAHLGVYGAACVTVNPYMGEDAVLPFLEEARGQERGGGVFVLVATSNPSSGDLQ
                     QSTDPPLFELAARLVALLGEQNGDYPDAGAVVGVTRPEVGARVREMLPDTLFLSPGYG
                     AQGGDASGVRSLLDRKGGGVLVNSSRSILYAYETSTADYKTAAREAARRTRDELGLVG
                     ARL"
     gene            complement(3021..3590)
                     /locus_tag="AC16CM_00470"
     CDS             complement(3021..3590)
                     /locus_tag="AC16CM_00470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3138328.1"
                     /translation="MKRLAIVFVLVLATALVYRSFGGAETGTGSLTLPQPAPHEGDNA
                     PAFDVKTVAGHDFELSDQGVYVLTFWSTLNKDSEEAEPGFEDLARKYENDGVSFAVVY
                     VNSAPNDDHVPYTMVLDSTGELVSKYNVKRVPRLFVIEDGKVEYAQDIYYEGYDKDLE
                     SAIEESLAEEKEQKTDSAAGDQTARHEKG"
     gene            3693..>4698
                     /locus_tag="AC16CM_00475"
     CDS             3693..>4698
                     /locus_tag="AC16CM_00475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3138329.1"
                     /translation="MDNSRTTISSGPVAAFATAFTPTFFLALALCVFAGAGPASAQQA
                     RDVGGPEVAARAWVLADLRSGDPLAGEDASRELPIASTTKIMEAIVVLENGDLDEEVT
                     VSGDAASYATRAYSNVGLLPGDTLSVRELLMAALISSGDDAAYALAEHVGGEGGVDDF
                     VGMMNEEAQALGLRNTHFENPIGLDDEGQYSSAEDLVTMARVAMRNPEFRRIVATEYT
                     SIYTADREIPLTNTNELLFSYRPVTGIKTGTTPAAGESLVSSASLGDESYICVVLDAR
                     EERFGASERTLRYGFVAHDRKDLVVKNKRYATLEAPYQRNEVVDLVAEDNVAGLVDAH
                     P"
BASE COUNT          867 a         1468 c         1553 g          810 t
ORIGIN      
        1 tcagcatcat caccacggac gatgcctact ccgaggaccc gcggaagata gccggggagg
       61 tcgaggcggg cgcggatcct tcgcggacgt gtgtcgagct cgaccggcag gcggccataa
      121 ggcgggcgct ccaggaggcg cgggagggcg acgtggtggt cgtggctggc aaggggcacg
      181 agaccgtcca gcacctgcca gaaggagacg tccccttcca cgatgccacc gtggtcgggg
      241 agctgctggc cgagatgggc gaacgaggga ggtagcgcgg gacttgcaga ggcagaccag
      301 gagcgaggta gtacgcaggc tcaagagcgt cgagggccac gtcagggggg tgcagaagat
      361 ggtcgaggag ggcgatgtag attacgctga tatcgtccag cagaccaacg ccatcttctc
      421 cgccataagg cgcataaaca ccgtgctcct caaagacttc gtcgaggagc gggccgaaaa
      481 agacggcgcc tctgacgcga tgataaacga cctgatcaaa gccatcgacc gggttacgaa
      541 gtagggtcat gagccgcgtg cgccccgaga ccctgctctt cgtgttgttg ctcaccgtgc
      601 cgctcgtggt tggcgtggcc ggcgttgctg ccttcctcct gatgagggcc gggttcggct
      661 ttctgatctg gggcgccgtg ccgttcgttg cctcgctgct cgcggtcgcc gggctctctt
      721 tcgtcctgag ccgggccgcg cgggcgtcca gggaccaatc ccccgaagaa ccaaggcgcg
      781 ggcgggatgg tgtatagtcc ggtacctatg gaggataccg ggagagaaca ggaagatctg
      841 gaagccaagg tcggggagct cgagaagctc accgactccc tgaacgaggt ctcagacgag
      901 gagctggtcg gcgcgcttgg cgaggccgtc gagctcctgt cggacatcaa cgcccgcata
      961 gaggacaggc tcgacgccgc gggagaggag tcgcgcgaga tcggcgacct ccttggggag
     1021 accgatttcg ggcccttcga tcgggccctc gaggagcacg aggccaagga acgcacctcc
     1081 ggtgagcctg gtgtctgacc tcgccccacg tatcagggag gcggccctac tggagggcga
     1141 cttcgtgctc tccagcggcg agcggagcag cttctacgtg gacaaatacc tcttctccac
     1201 cgagcctgat ctcctgcgcg acgtggccgg cgccctggcg gagcagatgc ccgacggggt
     1261 agagaggctc gccggcgtgg agctcggtgc cgtaccgctg gtcgtggcca cctcgcttgc
     1321 caccgggttg ccttacgtca tcgtccgcaa gagcgccaag gagtacggca gctccgccgg
     1381 ggagaacata gagggccgcc tcaacgcggg cgagaccgtc gttctggtcg aggacgtcgt
     1441 caccacagga acgcaggccg tcagggccgc cgggcacctg cgcgacgccg gtgtcgaggt
     1501 cgtctccata gtcgccgtgc tcgaccgtag ggaggaagat gggggcgaga tcggcgggtt
     1561 tccgttccgg gccctgctta gaatggacga attgcgggta gtcaaaagag attgaggccg
     1621 tatagtgtct ctggtatatt ccttcgcaga ccgggggaac gagagtggaa gcgaagttag
     1681 ggaggcagca gcgtggcgtt aaacaaaaca gcgttgatcg aggagatagc tagcaaggcc
     1741 aactcctcca agagcgacgc ccagaagttc ttcgatgcgt tcaccgaggt cgtcacagac
     1801 agcctcaaga gcggcgagca ggtccagatc accggtttcg gcaagttcta cgtgcaggag
     1861 cgcgaggcca ggcagggaat caacccccag accaaacagc ggatcaacat cgccgcctcc
     1921 aaggtcccca agttcaccgc cggcaacgcc ctgaaggacc agatcaagta gccagcacga
     1981 tgcttttgcg gggtcgcgga gtctgttccg cggccccttc ttttctgccc gcttcccgac
     2041 cttcacgtcc gcgatgcagg cgctcgtaga ggccgcccgc gcaagggata gcgtgctcgt
     2101 cgccggtatc gacccggtac cagcgcgttt tccgccgggg ctgcggggcc tacccgaggc
     2161 cgacgccacc ctgcgcttct gcaccggcat cctcgaggcc gtggaaccat acgcggcagc
     2221 cgtaaagctt caggcggcct tcttcgagcg tctcggcccg gctgggatgg aggtatacag
     2281 ggccctgatc gccgcctccg aggaactggc cttacccgtg atagcggatg tgaagcgcgg
     2341 cgatatcgga cccgtggcga ccgcgtacgc cgaggcgcac ctcggcgtct acggcgccgc
     2401 ctgcgtaacc gtcaacccat atatggggga ggatgccgtc cttcccttcc tcgaagaggc
     2461 ccgcgggcag gagaggggtg gtggcgtctt cgtcctggtt gccacctcga acccctcttc
     2521 cggggatctc cagcagagca cggacccgcc cctcttcgag ctggccgcca ggctggtcgc
     2581 gctgctggga gaacagaacg gcgattatcc cgacgccggc gccgtcgtcg gcgtgacccg
     2641 gcccgaggtc ggggcccgcg tcagggagat gctgcccgac accctcttcc tctcgcccgg
     2701 ctacggagcc cagggtggcg acgcctcggg cgtccgttcc cttctggacc gcaaaggggg
     2761 aggcgtcctt gtcaacagca gcagaagcat cctctacgcc tacgagacct cgaccgcaga
     2821 ctacaagacg gccgccaggg aagcagcccg ccgtacacga gacgagctag gcctcgtcgg
     2881 agcccgattg taggggcggt tcgcgaaccg ccagacgaag actgctctag aaaacgtgtg
     2941 cgacggcgaa gatggtcacg agcacggtcg cgacggcgac aaccaccgct agaatctgta
     3001 cgaggctctc tctggtcatc tcatcctttc tcgtgacggg ccgtctggtc tccggccgcc
     3061 gagtctgtct tctgttcctt ctcttccgca agcgattctt ctatggccga ttcgaggtct
     3121 ttgtcgtagc cctcgtagta gatgtcctgg gcgtactcta ccttgccgtc ttctatcacg
     3181 aacagccggg gcaccctctt gacgttgtac ttggatacga gctccccggt cgagtctaga
     3241 accatggtgt aggggacgtg gtcgtcgttc ggggcgctgt tgacgtatac gaccgcgaag
     3301 gagaccccgt cgttctcgta cttcctggcg agatcctcga agccgggctc cgcctcttcg
     3361 gagtccttgt tcagggtgct ccagaacgta aggacgtaga cgccctggtc cgagagttcg
     3421 aagtcgtgac ccgccacggt cttcacgtcg aaggcgggcg cgttatctcc ctcgtgcggg
     3481 gccggctggg gtagcgtgag cgaacccgta ccggtctcgg cacccccgaa agacctgtag
     3541 accagcgccg tggcaagcac cagaacgaaa acgatggcca gacgcttcaa gtgttttccg
     3601 gatctctact cgaagaaagc acgacgattg aattgtatcc gctagcagga accttgtcgc
     3661 cgggaccgaa ccatcgtagc ctctattatc ccgtggacaa ctccaggaca accatatcat
     3721 caggccccgt cgcggcgttc gccacggcgt tcaccccgac gtttttcttg gcgctggccc
     3781 tctgcgtctt tgcgggggct ggccccgcgt cggcccagca ggccagggat gttggcgggc
     3841 ccgaggtggc ggcgcgggcc tgggtgctcg cggacctgcg gagcggtgat cccctggctg
     3901 gagaggacgc ttcaagggag cttcccatag cgtcgaccac caagatcatg gaggccatcg
     3961 tcgtgctgga gaacggcgac ctcgacgagg aggttacggt ctccggggac gctgcctcct
     4021 atgcgacgcg cgcctacagc aacgtcggcc tccttcccgg cgacaccctg agcgtcaggg
     4081 aactgcttat ggcggccctg atctcctccg gagacgacgc cgcttacgct cttgccgagc
     4141 acgtgggtgg agagggcggg gtcgacgatt tcgtggggat gatgaacgag gaagcgcaag
     4201 ccctgggcct acggaacacc catttcgaga accccatcgg gctcgacgat gagggtcaat
     4261 actccagcgc agaagacctg gtcacgatgg cgcgggtggc gatgcgaaac cccgagttca
     4321 gaaggatcgt cgcgacggag tacacgagca tctacacggc tgacagggag ataccgctta
     4381 ccaacaccaa cgagctcctg ttctcctacc ggccggtgac ggggatcaag acgggcacca
     4441 cgccggccgc cggcgagtcc ctcgtctcct ctgcatcgct gggagacgag tcctacatat
     4501 gcgtggtcct cgacgcccgg gaagagcgtt tcggggcctc ggagcggacg ctacgttatg
     4561 gcttcgtcgc ccacgacagg aaagacctcg tcgtcaagaa caaacgatac gccacgctag
     4621 aagcgcccta ccagcgcaac gaggtggtcg accttgttgc cgaggataac gtggccggcc
     4681 tcgtggatgc gcatcccg
//
LOCUS       DBNGVD010000018         7754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3099791, whole genome shotgun sequence.
ACCESSION   DBNGVD010000018 DBNGVD010000000
VERSION     DBNGVD010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3099791"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="AC16CM_00480"
     CDS             complement(<1..1024)
                     /locus_tag="AC16CM_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3138330.1"
                     /translation="MILERLFIENYKQLRDPLELYPPEGAIGIVGANGAGKSTLFESI
                     LWAFFGSRGGGPRFANESIPWSGGSTKEPTTVEVTLATDGGSFTVRRRLKSGSTTAEA
                     LDGEGRVLVNGTADVTRWAEENLLGMDRTAFEATFYARQKELRFFAHDEGISRVRKIS
                     RLLGISSVETAQSFLREDRNNLRSEARVLEGRLAEADLDSLQTELESASKECQRLETD
                     LERVGEEHATAEEELRAAREVRTKLETAYREHSRLTSDLRGAEADLRRAQDRRVEAEK
                     LLAELAAAEEELNRLKPQTDRLPEVAAGLEKLDEERQLAERRDRERREHMQAQRRVSD
                     IEGEVYD"
     gene            complement(1021..2196)
                     /locus_tag="AC16CM_00485"
     CDS             complement(1021..2196)
                     /locus_tag="AC16CM_00485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3138331.1"
                     /translation="MVRIAHISDTHLGYTRYARLDPETSRNQREVDVQEAYGRAVDAI
                     LERDVDLVIHSGDVFGSVRPATHVIIGFLKQTARITLQANIPYLVAAGNHETPRLRST
                     TAALEYANLVNVISAHGFEIDYDLQDVDGTAVGVTLVPHGAVFGQGAVTPDRDADINI
                     LVTHGMVPGLEARQHEMGEANLQPGMLQSAFDYIALGHYHEFHEHKPNAYYAGATERF
                     GFGEVESTPGFAIVEFDGNGLAGVEHVEIPARPMLDLEKISAREMDAAELTEEVRKHT
                     SEADLDGAVVRQRVYDVRHGVASGMDRELLRDLGRRCLNFSLEVHAEERPEDVERNGT
                     QTAVFGPLGEEFAAFVRERKERGELEDAFAEEFLEKGRAYLTRAASEEPGAPEGSAA"
     gene            complement(2233..2568)
                     /locus_tag="AC16CM_00490"
     CDS             complement(2233..2568)
                     /locus_tag="AC16CM_00490"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3138332.1"
                     /translation="MRLLFDQNLSPRLVRLLSDVYPECSHVRDLDMAAASDTEVWTYA
                     AEHGYTIVSKDADFHQRSLVLGAPPRVVWIRRGNCSVSETADSDLWRWNVFTPERKRL
                     SWHSPKISR"
     gene            complement(2565..2792)
                     /locus_tag="AC16CM_00495"
     CDS             complement(2565..2792)
                     /locus_tag="AC16CM_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3138333.1"
                     /translation="MGYRDRITIDPDMRGGKPCIRGMRITVYDVLSYLASGMSQDEIL
                     ADFPDLEAEDIRASLAFAADFERRFVAEPSL"
     gene            complement(2848..3315)
                     /locus_tag="AC16CM_00500"
     CDS             complement(2848..3315)
                     /locus_tag="AC16CM_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIR3138334.1"
                     /translation="MKDAHIEAYWRGYLDTLPHDSPERDEPYVAEAWGDSSEMADELG
                     ALIASGIKTATCSALWEYEAEGEPVPVAGSKTIVLDGNNTPLCIVETTEVAVRPYDEV
                     DARFAYEEGEGDRSLAYWRDAHWRFFSRTLPDIGREPAPDMPLVCERFRVIHH"
     gene            complement(3334..5391)
                     /locus_tag="AC16CM_00505"
     CDS             complement(3334..5391)
                     /locus_tag="AC16CM_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138335.1"
                     /translation="MLHDDFLDQISGPIGRVVTSEESPATAHEFFFWTAENEAAQNLD
                     IGHIVAAESEDATAVAVLDDPRRYSDLQSFLDDFYAYDGDPSLEALSERAEILVFRAR
                     VLATKHRHERKKSKRPVRTGPVFFATNEAIEFALGTEDFSGHRIPMLLHENGNETDGL
                     PQRTPLYVDEEYLLGPEAGHLNITGMSGLSTKTSHALFTIASTFQTVKDKKVAALMFN
                     VKGADLLFLDKPVEAPEGDPELAERYERAGQRGLPIEDREMYRSLGLEVRPFENLKIF
                     APLRYGMESGEKRVFAEGIPARKLNTLRSARGEDSCVYPVVWELGDVLPYAGYVFEPS
                     DMDDKFRGFIEELRDRGIRTTADFYLLLDEIEDYFEAAREEGKNVSSWNGHNHMTIAK
                     VRNRFKVLPNKCGGLLAHGRVEHGDLPRADGPFEDNEMRVIDISQLTGVPQDLIVTSV
                     ISKIWELAETGNLGVDKLVIFVDELNKYAPSGNSRTSSLKDTLVDISARGRHLSLTLF
                     GAQQFRSKVEDEVIGNAATSLYGRIGDEELTNASYRSFSGTTREELLQLEKGRLLLRH
                     AHYAVPVFGRFPRPPVLMGKQGTDIFGQQKQDPASSVLAVMRNLTSRPPGIAAIRTEI
                     EGVPDEEVYEALDAVQAAHRTGHGAADPYKNFRWNLKRPARTGSGRRDASKILSGLDR
                     MRD"
     gene            complement(5392..6528)
                     /locus_tag="AC16CM_00510"
     CDS             complement(5392..6528)
                     /locus_tag="AC16CM_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138336.1"
                     /translation="MQSGSADDFVGKISRALEGAGRRGIHVTAGAGSCDLEAQQFGMF
                     PPEEEETPVGRPHHLESPVGPRDLGGHDGTSRLCYFLDGVQSSREIGRIEMTPVVVAT
                     VAAAIVNRFERRFSRMPLEAPPVVVQAVILPRSTGEPSVEAFWELLLAAGFSELDPDA
                     IPSSPHLILDSAEYIGGADPSDYVGMRESARGRVRTLRERLEGGMLREWEGDDRVLED
                     DDAWIAVDGQLRDVRESNRRAIGLIKSVARPEFVGRDVGMLMDLEPGMRTTSFVPDWQ
                     LRRDRSEQRTSWYLRMWPPQPGADALGSLMRVEAARETNPDQVDQISRWILAERAPLA
                     KPDPRWPAMIYPIRYVEKILKPLVQGSERAYARLERQLASNGRS"
     gene            complement(6562..7143)
                     /locus_tag="AC16CM_00515"
     CDS             complement(6562..7143)
                     /locus_tag="AC16CM_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138337.1"
                     /translation="MTEKGRGLDLPERVRGSLAGAVAERGVQARVISTQGRESMRPPQ
                     GHVIIAAEEPEIEAVRGPSRIVRRLEFYKSFYGPVVRLALSVYPMDGAPLSASTVLNV
                     SQVSGDTALAGLGRQKSIFLHFYSAQGDDLTYAFSKEIPNAPEQRGEAKKVLKMARDA
                     YSETPEERRSFRAAVGLAERQFELPVPEPDEQG"
     gene            complement(7140..>7754)
                     /locus_tag="AC16CM_00520"
     CDS             complement(7140..>7754)
                     /locus_tag="AC16CM_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3138338.1"
                     /translation="ADEPPERDLEAISRIPFDDEALRGSWGVEAMDRGLTGEDALREM
                     LLRPTANIAGIESGYTGPGAKTIVPSEALVKMDFRLVAGQSPGPVLGLIRAHLDRRGF
                     EDIEVVDLHGVEPAKTPIDSDVVGTAVETWRDLGREEAVVYPTIGGSGPTSLMVSELG
                     IPTIMTGSVANVDSRLHSPNEWIRLDDYFETIRYFVRFFERFAS"
BASE COUNT         1208 a         2580 c         2492 g         1474 t
ORIGIN      
        1 cgtcgtagac ctcgccctct atatccgaga cgcgccgctg cgcctgcatg tgctccctgc
       61 gttcccggtc gcgccgctcg gcgagctgcc tctcctcgtc cagtttctcc agcccggccg
      121 cgacctcggg gagcctgtcc gtctggggct tgaggcggtt caactcttct tcggcggccg
      181 cgagctcggc aagaagtttc tcggcctcga cgcgacggtc ctgcgcccgc ctcaggtcag
      241 cttcggcccc gcgcaggtcg gaggtgaggc gcgagtgctc gcggtaggcg gtctcgagct
      301 tcgtgcggac ctcgcgggcc gccctcagct cttcttcggc ggttgcgtgc tcttcaccaa
      361 cccgctcgag atcggtctcg aggcgctggc actccttgct ggcactctcc agctcggtct
      421 ggagcgagtc gaggtctgcc tcggcgaggc gtccctccag gacccttgcc tcggagcgga
      481 ggttgttccg gtcctcgcgc aggaaggact gggccgtctc cacgctgctt atgccgagga
      541 gtcgagagat cttgcgcacc ctgcttatgc cctcatcgtg ggcgaagaac ctgagctctt
      601 tctgcctggc gtagaaggtt gcctcgaagg ccgtcctgtc catgccgaga aggttctcct
      661 ccgcccagcg cgtcacgtca gccgtcccgt tgacgagcac cctgccctcg ccatcgagcg
      721 cctcggcagt cgtcgagccg ctcttcagac gccgccggac ggtgaatgac ccgccgtccg
      781 tcgccagcgt cacctcgacg gtggtcggtt ctttggtaga gccgccgctc caggggatgg
      841 actcgttggc gaaacgcggc ccgccgccgc gagagccgaa gaaagcccac aggatgctct
      901 cgaagagggt tgacttgccc gccccgttcg cgcccacgat gcctatggcc ccctcgggcg
      961 ggtatagctc tagggggtcc cgcagttgct tgtagttctc gataaagagg cgctccagga
     1021 tcacgcggcg ctgccctccg gagcacccgg ctcctcgcta gccgcccgcg tgaggtaagc
     1081 cctacccttc tccaggaact cctctgcgaa agcgtcctcc agctcgcccc gctccttgcg
     1141 ctccctgacg aaggccgcaa actcctcgcc cagcggcccg aagacggccg tctgggtgcc
     1201 gttcctctcc acgtcctccg gcctttcctc ggcgtggacc tccagcgaga agttcaggca
     1261 gcggcgcccg aggtcgcgca agagctcgcg gtccatgccg ctggcgacgc cgtggcgcac
     1321 gtcgtagacc ctctggcgca cgacggcgcc gtccaggtcg gcctccgagg tgtgtttgcg
     1381 cacctcctcc gtcaactctg ccgcgtccat ctcgcgggca ctgatcttct ccagatcaag
     1441 catcgggcgg gccggtatct ccacgtgctc cacgccagcg agcccgttgc cgtcgaactc
     1501 cacgatggcg aaaccgggcg tagattcgac ctccccgaac ccgaaccgct ccgtcgcgcc
     1561 ggcgtagtag gcgttcggct tgtgttcgtg gaactcgtgg tagtggccga gcgcgatgta
     1621 gtcgaaggcg ctctggagca tgccgggctg caagttcgcc tcgcccatct cgtgctggcg
     1681 cgcttcgagg cctggcacca tcccgtgcgt cacgaggatg ttgatatccg cgtcgcgatc
     1741 cggtgtgacc gcgccctggc caaagaccgc cccgtgcggg accagcgtca cgccaacggc
     1801 cgtgccgtcc acgtcctgga ggtcgtagtc gatctcgaag ccgtgggcgg agatgacgtt
     1861 tacgaggttc gcgtactcca gggccgccgt ggtggagcgc aaccgcggcg tctcgtggtt
     1921 tccggctgcg accaggtagg ggatgttggc ctggagcgtg atcctggccg tctgcttcaa
     1981 gaagccgatg atcacgtgcg tagccggcct gaccgagccg aagacatcgc cggagtggat
     2041 gaccaggtcc acatcccgct ccagaatggc gtcgacggcc cgtccgtagg cttcctgcac
     2101 gtccacctcg cgctggttgc ggctagtctc gggatcgagc ctcgcgtacc tggtgtatcc
     2161 caggtgtgta tccgagatgt gggcgatcct tacgataccg ctcctcctga gtagatcgac
     2221 ttattttacg catcaccgtg agattttagg agagtgccag gaaagccgct tcctctctgg
     2281 cgtgaaaacg ttccatcgcc ataaatcgct atcggccgtt tccgatacag agcagttgcc
     2341 ccggcggatc cagaccaccc tcggcggtgc tcctagtaca agactgcgtt gatgaaagtc
     2401 cgcgtctttg gagactatgg tgtaaccgtg ctcagcggca taggtccaaa cctcggtatc
     2461 gctcgccgcg gccatgtcca ggtctcggac gtgggaacat tctgggtaaa cgtcggatag
     2521 aaggcgaacg aggcgcggcg agaggttctg gtcgaagagg agcctcaaag agagggctct
     2581 gccacgaacc tgcgctcgaa gtctgccgca aaagccaggc tcgcacggat gtcttccgcc
     2641 tccagatcag ggaagtcggc cagtatctcg tcctgggaca taccggatgc aaggtaactc
     2701 agcacatcat aaacggtgat ccgcattcca cggatgcacg gcttcccacc tctcatgtcg
     2761 ggatctatgg taatgcgatc acgatagccc atgattggag tctacgttgc gatctggggc
     2821 ggttcaagga tcaagataag ctggatctca atggtggatc acacgaaacc gttcgcacac
     2881 caacggcata tccggtgctg gttctctgcc gatgtccggt agcgtgcgcg agaagaagcg
     2941 ccagtgtgcg tcgcgccagt acgccagcga acggtcacct tctccctcct cgtaggcgaa
     3001 ccgggcatcg acctcgtcgt aggggcgcac ggcaacctct gtcgtctcca cgatgcacag
     3061 aggagtgttg ttcccgtcga ggacgatggt cttcgaccct gccacgggta ccggctcccc
     3121 ttccgcctcg tactcccaca gcgcggagca ggtagccgtc ttgatgccac tcgcgatcag
     3181 agccccaagc tcatccgcca tctcggaaga gtctccccac gcttcggcca cgtacggctc
     3241 gtcgcgttcc ggtgaatcgt gcggaagcgt atccaggtaa ccccgccagt atgcttctat
     3301 gtgggcgtcc ttcattgacc tgtccgagcg aagctagtct ctcatccggt ccaggccgga
     3361 gagtatcttg gacgcgtcgc ggcggcccga gcccgtcctg gcggggcgtt tgaggttcca
     3421 gcggaagttt ttgtacgggt cggccgcgcc gtgtccggtg cggtgggccg cctgcacggc
     3481 gtcgagtgcc tcgtacacct cctcatccgg aactccctct atctcggtcc ggatcgcggc
     3541 gatgcctggc ggcctggagg tgaggttgcg cataacagcg aggactgagg aggccggatc
     3601 ctgcttctgc tggccgaaga tgtccgtgcc ttgcttgccc atgagcacag ggggacgcgg
     3661 gaagcggccg aagacgggga cggcgtagtg agcatgcctg agaagaaggc ggcctttctc
     3721 tagctgcaac agctcttcgc gggtcgtgcc cgagaaggag cggtagctcg cgttggtcag
     3781 ctcctcgtcg ccgatgcggc cgtagaggct ggtcgcggcg ttgccgatca cctcgtcttc
     3841 caccttgctc ctgaactgct gtgccccgaa gagcgtgagg ctgaggtgcc tgcctcgtgc
     3901 cgaaatatct accagcgtgt ccttgaggga cgaggtcctc gagttgccgg agggagcgta
     3961 cttgttcagc tcgtccacga agatgaccag cttgtctacc ccgaggttgc ccgtctcggc
     4021 gagctcccag atcttcgaga taacgctggt cacgatcagg tcctgcggca cgcccgttag
     4081 ctgggagatg tcgatgacgc gcatctcgtt gtcttcgaac gggccgtcgg cgcggggcag
     4141 gtcgccgtgc tcgacgcgcc cgtgcgcgag cagaccgccg cacttgtttg ggaggacctt
     4201 gaagcggttt cggaccttgg cgatggtcat gtggttgtgg ccgttccagg aggagacgtt
     4261 cttgccctcc tcgcgggccg cctcgaaata gtcctcgatc tcatctagca gcaggtagaa
     4321 atcggccgtg gtcctgatgc ccctgtcgcg gagctcctct ataaagccgc ggaacttgtc
     4381 gtccatgtcc gaaggctcga acacgtaccc cgcgtacggt agtacgtccc cgagctccca
     4441 gaccaccggg tatacgcaag agtcctcccc gcgcgcgctc cttagggtgt tcagcttgcg
     4501 cgccggtatg ccctcggcga agacccgctt ctcacccgac tccatcccgt agcgcagggg
     4561 ggcgaagatc ttgaggttct cgaagggcct gacctcgagg ccaagggacc ggtacatctc
     4621 ccggtcctcg atcggcaacc cccgctgacc ggccctctcg tagcgctccg cgagctcggg
     4681 gtcgccctcc ggtgcctcga cgggcttgtc cagaaagagg aggtccgcgc ccttcacgtt
     4741 gaacatcagg gcggcgacct tcttgtcttt tacggtctgg aaggtgctgg ctatagtgaa
     4801 gagggcgtgg ctggtcttgg tggagaggcc gctcatgccc gtgatgttga ggtggccggc
     4861 ctccgggccg agcagatact cctcatccac atagaggggc gtgcgctgcg gcaggccgtc
     4921 cgtctcgttg ccgttctcgt gcagcagcat ggggatgcgg tggccggaga agtcctcggt
     4981 gccgagggcg aactcgatgg cctcgttcgt ggcgaagaag acggggccgg tgcgcaccgg
     5041 ccgctttgac ttcttgcgct cgtggcggtg cttcgtggcg aggacccgcg cgcggaagac
     5101 cagtatctcg gccctctccg agagcgcttc cagggatggg tccccgtcgt aggcgtagaa
     5161 atcgtcgagg aaggattgca ggtcggagta gcggcggggg tcgtctagca cggcgacggc
     5221 cgtggcgtct tccgactcgg ctgccactat gtgcccgatg tccaggttct gggccgcctc
     5281 gttctcggcg gtccagaaga agaactcgtg ggccgtggcc gggctctcct cgctggtcac
     5341 gacgcggcca atggggccgg agatctggtc cagaaagtcg tcgtgcagca tctagctcct
     5401 cccgttcgat gcgagttggc gctcaaggcg tgcgtaggcc ctttcggagc cctgcacgag
     5461 cggtttgagg atcttctcca cataccttat ggggtagatc atagcgggcc acctcgggtc
     5521 gggcttggcg agcggtgccc tctcggcaag gatccaacgc gatatctggt cgacctgatc
     5581 gggattcgtt tcccgggccg cctcgacgcg catgagcgaa ccgagggcgt cggcccccgg
     5641 ctgcggcggc cacatcctga ggtaccagga tgtacgttgc tcgctcctgt cgcggcgcag
     5701 ttgccagtcg gggacgaagc tggtcgtccg catgccgggt tcgaggtcca tcagcatgcc
     5761 cacatccctg ccgacgaact cggggcgggc cacgctcttt atgaggccga tagcgcgccg
     5821 gttcgactcc ctcacgtccc tgagctgccc gtccaccgcg atccaggcgt cgtcatcctc
     5881 gaggaccctg tcatcgccct cccattccct gagcatcccg ccctccaacc gttcgcgcag
     5941 cgtcctgacc ctacccctgg cgctctccct catgcccaca taatcggacg gatccgcccc
     6001 gcctatatac tcggccgagt cgaggatgag gtggggggag gaggggatcg catctgggtc
     6061 cagctcggag aagcccgccg cgaggagcaa ctcccagaag gcctccacgc tcggctcgcc
     6121 ggtgctgcgg ggtaggatca cggcctgcac cacgacgggg ggagcttcga gcggcatgcg
     6181 cgagaaccgc cgctcgaagc ggttgacgat cgccgcggcc acggtggcga ccaccacggg
     6241 cgtcatctct atgcgcccga tctcccgcga gctctgcacc ccgtcgagaa agtagcagag
     6301 ccgcgaggtc ccgtcgtgcc caccgaggtc cctcggcccc acgggggact ccaggtggtg
     6361 cggcctaccc accggtgtct cctcttcctc gggcggaaac atgccgaatt gctgggcctc
     6421 gaggtcgcat gatccggccc ccgcagttac gtggatgccc ctgcgcccgg ccccttccag
     6481 ggcccggctt atcttcccta cgaagtcgtc cgcgctcccc gactgcatac gcggcagtat
     6541 acgcgggcag gccccggaag actatccttg ttcgtcaggt tcaggtacag gaagctcgaa
     6601 ctgacgctcg gcgagcccga ccgcggccct gaagctcctg cgctcctcag gggtctccga
     6661 gtaggcgtcg cgggccatct tgagaacctt ctttgcctca ccgcgctgct cgggggcgtt
     6721 tggtatctcc ttgctgaacg cgtaggtgag atcgtcgccc tgggcggagt agaagtgtaa
     6781 gaagatgctc ttctggcggc ctagcccggc gagggccgtg tcgccgctga cctgggagac
     6841 gttcaggacc gtgctggcgg agaggggagc gccgtccatc gggtacacgc tcagggccag
     6901 ccggacgacc ggaccgtaga aggacttgta gaactccagc cgtcgcacga tgcgggaggg
     6961 gccccggacc gcctctatct ccggctcctc tgcggcaatg atcacgtgcc cctgcggggg
     7021 ccgcatcgac tcgcggccct gcgtggagat cacgcgcgcc tgcacgcccc tctcggccac
     7081 cgccccggcg aggctcccgc ggacgcgctc ggggagatcc aggccccgtc ccttctcggt
     7141 catgaggcga acctttcgaa gaagcggacg aagtagcgga tggtctcgaa gtagtcgtcg
     7201 agccggatcc actcgttcgg agagtggagg cggctgtcca cgtttgccac gctgccggtc
     7261 atgatggtcg gtataccgag ctcggagacc atgagcgagg tcgggccgct ccccccgatg
     7321 gttgggtaca cgacggcctc ctcgcggccg agatcccgcc aggtctccac cgccgtaccc
     7381 accacgtcgg agtcgatggg cgtcttggcc ggctcgaccc cgtgcaggtc cacgacctct
     7441 atatcctcga agccgcgcct gtccaggtgg gcccggatca ggccgagcac cggcccggga
     7501 ctctggccgg cgacgagccg gaagtccatc ttcactaacg cctcggaggg cacgatggtc
     7561 ttcgcccctg ggcccgtata gcctgactct atgccggcga tgttcgcggt ggggcgcagg
     7621 agcatctcgc gcagcgcgtc ttcgcccgtg agcccgcggt ccatcgcctc cacgccccag
     7681 gacccccgca gcgcctcgtc gtcgaaggga atgcgagaga tagcctccag gtcccgctcc
     7741 gggggttcgt cggc
//
LOCUS       DBNGVD010000019         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4739635, whole genome shotgun sequence.
ACCESSION   DBNGVD010000019 DBNGVD010000000
VERSION     DBNGVD010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4739635"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1795
                     /locus_tag="AC16CM_00525"
     CDS             218..1795
                     /locus_tag="AC16CM_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HIR3138339.1"
                     /translation="MERDLPDSPARRAGRIARVGARYGFGFVFGRRFLPRRRREEPER
                     VGTRLRLSLEELGPTFTELGRFLAVRRDLIPPNITAELARARVVVKPIAFPEARAYVE
                     KELGNTLERLFLEFGEVPDRVGPFTQAHRAVLPGGRPALVVFNRSGVRRDLLAMRPVA
                     DLTRRRLGEGLPLDPSVTVAEFIAHVNQRRDMFFAAQISHRLQGMESFRLLVPDVYRD
                     YSTGRCITFAAPANQAPVEETHRLEISDALIQLALTEGVILSDLSLARFALDGVTGRV
                     WLADPTEAFGVDPERLRGLAEVLAAVRRGDVDDAIRSLPLAGSSIPHDNSALRREMRE
                     TLGSLGGPLWREHSLQQVRGSSLESVRRGGARLHVEVAQLSDFLVEAERFQTETKTQI
                     TPAAEASEVLISRYRDPTYIAGRLARAVARPDAFADYPRQVHAILDELKDGEIEVRFR
                     HAGLDDLISKVDILANRLVFAFLIAALIIGSSMLGIFLKQGTKFLGVSIFGLIGFVFA
                     AVLGLLLLVGIIRSGRL"
     gene            complement(2096..2569)
                     /locus_tag="AC16CM_00530"
     CDS             complement(2096..2569)
                     /locus_tag="AC16CM_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138340.1"
                     /translation="MGITTGRLLIVGLVLLVGLVSRVTYEQLIHPEEPALAQSSQDDC
                     SSFDSQQSAQAALERDPSDPDDLDADGNGSACDDYDYSTTSNTQSSPLPSSASPGSSS
                     TSDSSTSSPSPSSGSRPQGDRTLLDAGGPTSGPVPLMPGGTCPPEFPTKQQDLCY"
BASE COUNT          433 a          855 c          905 g          479 t
ORIGIN      
        1 cggcctgcac ccttccgctc tagagattcg atgcccggaa gcagtgggtt gtcgaagcca
       61 ccgttacgga tcgagacgac cagccgaaag cgaggcgaag ccgaagcgtt gccgacaagc
      121 gagggcgtca gcccgagcgt gccgacaagc ggaggccgaa ggccgtagcg tgctgagagc
      181 gtaggcgtta gccgtagcgt gctgataagc ttgtctcatg gaacgggatt tacccgacag
      241 cccggcgcgt agggccggaa ggatcgcccg tgtgggggcg cggtacgggt tcgggttcgt
      301 tttcgggcgc cggtttttgc ccaggcgccg cagggaggag ccggagcggg tcggcacgag
      361 gctgcgcctc tctctcgaag agctgggccc gacgttcacg gagctgggac gctttctggc
      421 ggtgaggcga gacctgatcc cacccaacat aacggccgag ctggcgcgcg ccagggtcgt
      481 tgtgaagcct atcgcctttc cggaggcgag ggcctacgtg gagaaggagc ttggcaacac
      541 gctggagcgg ttgtttcttg agttcgggga ggtgccggac agggtcgggc cgtttaccca
      601 ggcgcaccgg gccgtgctac cgggggggcg tccggcgctg gtggtcttca accggagcgg
      661 ggtgcgaagg gacctactcg cgatgcgccc cgtcgccgac ctgacgcgcc ggaggctcgg
      721 cgaggggttg cccctggacc cttccgtcac cgtggccgag ttcatcgccc acgtcaacca
      781 gcggcgcgac atgttcttcg cggcccagat ctcgcaccgc ctgcagggga tggagagctt
      841 ccggctgctg gtccccgacg tctacaggga ctactcgacg ggccgctgca taacgtttgc
      901 ggcgcccgcg aaccaggccc ccgtggaaga gacgcaccgg cttgagatct ccgacgcgct
      961 catccagctc gcccttaccg aaggcgtcat cctaagcgac ctgtccctgg cacgtttcgc
     1021 gctcgatgga gtgacgggaa gggtctggct tgccgacccg acggaggcat tcggtgtcga
     1081 tccggagcgg ctgcggggcc tggccgaggt cctggccgcc gtgcgccgag gcgacgtgga
     1141 cgacgcgatc cgctcgctcc ccctggccgg aagctcgatc ccccacgaca actcggccct
     1201 caggcgcgag atgcgggaga ccctgggctc cctgggagga ccactctgga gagaacactc
     1261 cctccagcag gtaaggggct cgagcctgga gtcggtaaga aggggcggcg cacggctaca
     1321 cgtcgaggtc gcccagttgt ccgattttct ggtcgaggca gaacggttcc agaccgagac
     1381 aaagacgcag atcacgccgg cggccgaagc ctccgaggtt ctgatctccc gctacagaga
     1441 cccaacctac atcgccggcc gactggcccg cgcggtggcc cgtcccgacg ccttcgcgga
     1501 ctacccgcgt caggtccacg ccatcctcga cgagctaaaa gacggcgaga tagaagtccg
     1561 tttcaggcac gccggcctgg acgacctgat ctccaaagtc gatatcctgg ccaaccgcct
     1621 ggtcttcgcc ttcctcatag ccgccctcat aataggctcg tcgatgcttg gcatcttcct
     1681 caaacaggga accaagttcc tgggcgtaag catcttcggc ttgatcggct tcgttttcgc
     1741 cgccgtcctc ggcctgctcc tcctggtcgg cataattcgc tcgggtcgcc tgtgacgagc
     1801 cgctagcggc tcgtcagcac gccggctccg ctccaccagc ttgtcagcac gctccagcct
     1861 gcgtgcggct gtcgcttgtc agctcgcttc gcctcgcttt cggccagtcg tctcgatccg
     1921 taacgacagc ttcgactacc aacctgtcct gagcatcgaa tctctagagc ggcagggagc
     1981 aggccgcaga gcgatctggc tgaaagcaag ccaggcggag ccgggcgaag cgttgctgac
     2041 aagcgtaggc cgaaggccgt agcgtgctga gagcgtaggc gttagccgga gcgcgctagt
     2101 agcagaggtc ctgttgcttt gtcgggaact ctggcgggca ggtgccgccg ggcatgagcg
     2161 gcacagggcc gctggtgggg ccaccggcgt caagcagggt gcggtctccc tggggtctcg
     2221 atccggagga aggggacggg ctgctcgtcg aggagtcgct cgtcgaggag ctgcctggtg
     2281 aggcgctgct tggcagtggg ctgctctggg tgttgctggt agtgctgtag tcgtagtcgt
     2341 cgcaggcgct gccgttaccg tcggcgtcga ggtcgtcggg atcgctcggg tcacgttcga
     2401 gggcggcctg ggccgactgc tgggaatcga agctcgaaca gtcgtcctgg ctcgattgcg
     2461 ccaaggctgg ctcttccggg tggatcagct gctcgtaggt gacacgcgag acgagaccga
     2521 caagtagcac cagccccact atcaacagcc tcccggttgt gattcccatc ctgccccgta
     2581 ttgtacccca cctcgttttg ggatgatttt tcttgcggcc ggcgtccctc acccccgcgg
     2641 cgcttgccaa ccctctgcaa agcctgtcca cc
//
LOCUS       DBNGVD010000020         8540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1642832, whole genome shotgun sequence.
ACCESSION   DBNGVD010000020 DBNGVD010000000
VERSION     DBNGVD010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1642832"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..1945)
                     /locus_tag="AC16CM_00535"
     CDS             complement(407..1945)
                     /locus_tag="AC16CM_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3138341.1"
                     /translation="MALLALAALGTMMLLALQPTPAWAATTFTVTRPGDEGDINLSNA
                     RCDASNDSGDQCTLRAAIEEANDTDGDDTIDFDIGSDTGVKTISPDVPLPPITDTVIV
                     NGYSQPGARPNTLEEGNDADLKVQLDGTGTETDPSGIGEVSGIVIRAADSVIKGLVIK
                     KFDDYGVHISGIGSDEVTGNRIEGNFIGTNADGTADRGNVEDGVLIAQGANNNTIGGP
                     RPGRRNVISGNGDEGISIFSDATGNRVEGNHVGTTADGTGDLGNDGVGVLVYAAEDNT
                     IGGTVAGKRNVISGNESSGVEIFADAKDNWIEGNFIGTTADGSGNLGNAEDGVTLFGS
                     DFAVVGGTAEGAGNRIAHNGDDGVSILLDYQEGNQILSNSIYENGEQGIDLGNDGVTQ
                     NDPGDVDSGANGLQNFPRLLSATRDSATGDTTISGELRSNPGQDFAVQCFLTDGTHGS
                     GHGEGLLLLETTVASTNANGRVNFSCVSSSPTSGQTVTATATNVLTGDTSEFSRNRTV
                     TTVP"
     gene            complement(2488..2652)
                     /locus_tag="AC16CM_00540"
     CDS             complement(2488..2652)
                     /locus_tag="AC16CM_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138342.1"
                     /translation="MTRIILGKVMWLGRTMVFCVVPEVPLAGTGGGNARASRRARDQV
                     QETSTERMVD"
     gene            complement(2711..3532)
                     /locus_tag="AC16CM_00545"
     CDS             complement(2711..3532)
                     /locus_tag="AC16CM_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138343.1"
                     /translation="METAKKLFSRVLWVGRGTATVMGLVVFLALVLGVTTSALAGTGV
                     GATFDLGRTNTVDALSRLVGGTNDALLRIDNGNDGASATALDLRVEPGHAPMKVDSDT
                     KVVGLNVDEVDGKSESSFLGANEKASDADHADFADKAAGATFADRADQAAKADEATHA
                     ARADRAANAGMVDGLNADEIGVNGRSRVFVESDSNSVSPKSATAYCPSGKVVVGTGYY
                     IVGGTVGNGGNQETNVVANAVVPELTNVEVIAYEENPTNADWSVYAYAVCAQQSF"
     gene            complement(3615..3809)
                     /locus_tag="AC16CM_00550"
     CDS             complement(3615..3809)
                     /locus_tag="AC16CM_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138344.1"
                     /translation="MLETCYCGRTGELEDRNLVSDDDGREALECPRCGHLDHLSWLQD
                     PRSVFEEARRRTGKREMSAA"
     gene            complement(4001..6442)
                     /locus_tag="AC16CM_00555"
     CDS             complement(4001..6442)
                     /locus_tag="AC16CM_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3138345.1"
                     /translation="MAVTDLREGRVRARPRADLPAQASSFVGRERELDYLGHMLASGR
                     LLTLTGPGGCGKTRLALQAAAHLAEDFEDGVVFVELATLSNPELVAEGVARALGLRMA
                     GVSPAGALLDYLESRATLLVLDNCEHLIEACAGLADELLRSCPGLKILATSREALRIP
                     GERAWLVPSLSLPDVREEIVPERLTRHEAVRLFVERAAAAVPGFQLDEHNAPAVARIC
                     QRLDGIPLAIELAAVKVRVLSASQIAARLDDRFLLLTDGSRVALPRQRTLLATMDWGH
                     ELLSERERALFRRISAFSNGFTLEAAEEVCSEGGVERGEVLELLGRLVDKSLVLVETR
                     GENARYRMLESVGQYASRKLEGSGEERSVRSRHAGFYLRLAERAEPALSGPDQGAWID
                     RLDAEVGNLRAAMSWFRTAEDTEAGLRLASALWGFCHARGYYETGRAWLEDALATSGD
                     PTSLRAGALRGAGLLAFLQCDYTGAGEHLGHSLSLYKDLADRCGVASVKDVLGGVARE
                     QGDYGRARKLHEESLALWRELEDEHGIAESLYYLGLAAWLNGENERAGDLSARALDMT
                     RDSGDAAGILSSLLNLGSVALYEEDYDRAETLLDESLAISREGGYREGVGWALNQLGM
                     VAYHRGDLEQAGWYLRESLAVHEDLGDLGRVASVLEALAEAAAASKHFGRSARLFGAA
                     EALRESTGVPVPPCENPEYDRALAAVRTRVGDGEFSLLRAEGRSMKLEAAVSLALEPD
                     VAPPTLASQGVLSARETEVLGLVAEGLTDQEVAQRLYLSPRTVGQHLSSVYRKLGVIS
                     RTAATHEATERGILK"
     gene            complement(6731..7387)
                     /locus_tag="AC16CM_00560"
     CDS             complement(6731..7387)
                     /locus_tag="AC16CM_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3138346.1"
                     /translation="MHSILLVEDHASFRQTLAYVFDQQPDFQVVAQAGTLDEARAVMD
                     GSEVADLGVIDLTLPDGAGTELIAELREANPDFAALVLTASLDRADHARAIEAGAASV
                     LHKSADVDDILDATRRLGDGEPLISQQELVELLRLAGQSREEELEARASIGQITPRER
                     EVLNSLAEGLSNKEIAAKLHMSVDTERTHMMNILNKLGVHSRIQALLFAARHGLVEIR
                     "
     gene            complement(7465..>8540)
                     /locus_tag="AC16CM_00565"
     CDS             complement(7465..>8540)
                     /locus_tag="AC16CM_00565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3138347.1"
                     /translation="HQDITEQRVAEEERRQAEKKYRSIFENAVEGIFQTTVEGRFITA
                     NPAMARIFGYDSSEELLGAISSIGDQLYVEPERRKEFTKVVVRDGLVSGFETQVLRKD
                     GTPAWISANARAVYGDEGEVVGYEGTVEDITERKRAEEALREIREAERRRIARELHDV
                     VLQDLTYALQSVQVSRRMPEGANRAREADQQVEAMKRAVGGLREAIYDLRLESIRERS
                     VIRAVASIVQLNRQIGGGCEFELIVDDGFPAGITGESGVEVVRVVQEALANVRRHSGA
                     EHAVVTLGAAGDEILVEVEDDGGGFGPQTAFGMGLTGMRERVAALGGKLEVEGREGVG
                     TRVFLRVSLAALTGGEAGELSST"
BASE COUNT         1357 a         2981 c         2563 g         1639 t
ORIGIN      
        1 ggggggtgcc tcggggcgca cgatcctgaa gctgcctccc tgactctgcg ccatcgcggt
       61 cccggcgagg atgagcccca gcgccaggat caaccccata acgagcagcg cgcgtctagt
      121 tattggattc atctggtctc ctatccaggt agacactcct gcaaaacgct gatactgctg
      181 tgcgcttcat cttcttcggc gctctccttt ccccatcgta tcgcccccat cgtacatgtt
      241 gcattctttg tttcaaaata ctttgcgttt gcgcagagga tggcgttcgt cccggcattg
      301 acccgccgga tggtaccccg ggtctcctgc tcgtttcatc catcctcccc tttctcgtag
      361 cctatcggct ggtttaccgg gatcggaccg tttgaagccc cgggcctcac gggacggtgg
      421 tgacggtcct gttcctggag aactcggagg tatcgccggt aaggacgttc gtcgcggtcg
      481 cggtcaccgt ttgccctgat gtcggagagc tactaacgca ggagaagttc acgcgcccgt
      541 tggcgttggt cgaggcgacc gtcgtttcca gaaggagcag accctcaccg tgacccgagc
      601 cgtgcgtccc gtccgtcagg aagcattgga cagcgaaatc ctggccgggg ttgctcctga
      661 gctcaccgga gatggtcgta tcgcctgtcg cggagtccct cgtggccgag agtagacggg
      721 ggaagttctg caggccgttt gcaccggagt cgacatctcc ggggtcgttc tgggtcacgc
      781 catcgttgcc gaggtctatg ccttgctcgc cgttctcgta gatagagttg gagaggatct
      841 ggttgccctc ctggtaatca agcaagatcg ataccccgtc atcgccgttg tgggcgatgc
      901 ggttgcccgc accttcggcc gtccccccga ccacggcgaa gtccgacccg aagagggtta
      961 cgccgtcttc ggcgttgccc aggttaccgc tcccgtcggc cgtggtgccg atgaagttgc
     1021 cttcgatcca gttatccttc gcgtcggcaa agatctcgac gccgctcgac tcgttgccgg
     1081 agataacgtt gcgcttcccc gccaccgttc caccgatcgt gttgtcttct gccgcataga
     1141 ccagtacgcc gaccccgtcg ttgcccaggt cgccggttcc gtctgccgtg gtgcccacgt
     1201 ggttaccttc gaccctgttc ccggtagcat cgctgaaaat gctgatcccc tcatcaccat
     1261 ttccggagat gacgttgcgc ctcccaggcc ggggtccccc gatcgtgttg ttgttcgccc
     1321 cctgagcgat tagcacacca tcctctacgt tccccctgtc ggccgtccca tcggcgttcg
     1381 tgcctataaa gttgccctcg atcctgttgc ccgtgacctc gtcgctgccg attcccgaga
     1441 tgtggacgcc gtaatcgtcg aatttcttga tcaccaaccc cttgatcacg ctgtcggccg
     1501 ccctgatcac gatcccgctc acctccccga tcccgctcgg gtcggtctcc gtgcccgtcc
     1561 cgtcgagttg caccttgagg tccgcgtcgt tgccctcttc cagcgtgttg ggtctcgctc
     1621 ccggctggct gtagccgttt acgatgacgg tatcggttat ggggggaagc ggtacatcgg
     1681 gagagatggt cttgaccccc gtgtcgctac cgatgtcgaa gtcgatggtg tcgtccccgt
     1741 ctgtgtcgtt tgcctcctct atggcggccc tgagggtgca ctgatcgccc gaatcgttcg
     1801 aagcgtcaca cctggcgttg ctgaggttga tgtccccctc gtcgccgggt ctcgtgacgg
     1861 taaaggtggt cgcggcccag gctggggtgg gctggagcgc cagtagcatc atcgtcccga
     1921 gcgccgcgag agcgagcagc gccatccacg cggtcctgcc actcgtcgaa gctttctctc
     1981 gtccgtcttg tctcttatac aacacgagcg acatttgatc tccttgaagc agatagaagg
     2041 acgagcgtcc cccggaggag gcatttcgat cggtattcac cgggttaccc ctggcgagag
     2101 cggcaccacg gggatgcgtc ttccggcggc gcggtaccat ctccccgggt tcgtcctgat
     2161 ggagaccctc gtacacgtca cgtagaggca gccgcgcata ccagagcctc taatttccgc
     2221 cgcccggtcc gccaaaaagt accgacactc cccttgtaat gccagcctcc atcggggatc
     2281 gcgccgcgcc agaatcgtgg tctggccgtg tccttcgtgg agaagtggcg tcgctggcct
     2341 cctccgtggc caccgcatta cgcgaacgcc gccgaagcag ccggccgatg agcggaatcc
     2401 ccgagccgat gccgggcgag tctccgcgcg tgggcacacc agcggatcgg tagttcgcgg
     2461 cctgatctgt ttgtgttgcc tgcgtcatca gtcaaccatc ctttcggtac tcgtctcctg
     2521 aacctgatcc ctggcgcgtc gcgaagccct cgcgttgccc ccacctgtcc ctgcgagggg
     2581 aacttcgggc accacgcaga acaccatcgt tctgcccagc cacataacct tgccgaggat
     2641 gattctcgtc acctttcacc gttcctctcc agaagcgcga catgagggtt cgggacatgc
     2701 tcatccagcc tcagaaagac tgctgggcgc aaaccgcgta ggcgtacact gaccagtcgg
     2761 cgttggtcgg gttctcctcg taagcgatca cttctacgtt cgtaagttcg ggtactacgg
     2821 cgttggccac gacgttggtc tcctggttcc cgccattgcc gaccgtgccg ccgactatgt
     2881 agtagccggt tcccacgacc acctttcccg acgggcaata cgcggttgcg ctcttgggcg
     2941 agaccgagtt ggaatcgctc tcgacgaaga cccgcgatcg gccgttcacc ccgatttcgt
     3001 ccgcgttcaa gccgtccacc atgccggcat tggcggcgcg atccgccctt gcggcgtggg
     3061 ttgcttcgtc ggccttggcg gcctgatccg cccgatccgc gaaggttgcg ccggcggctt
     3121 tgtcggcgaa gtcagcgtgg tccgcgtcgg aggccttctc gttggctccc aggaaagagc
     3181 tctcgctctt gccgtcgacc tcgtccacgt tgagacccac caccttggtg tctgagtcga
     3241 ccttcatggg cgcgtggcct ggctcgaccc gcagatccag ggccgtcgcc gaggccccgt
     3301 cgttgccgtt gtctatacgg agaagggcgt cgttcgtgcc gcccaccaac ctgctcaagg
     3361 cgtcaacggt attggtcctg cccaggtcga aagtcgctcc cacacccgtg ccggccagag
     3421 cgctcgtcgt cacccccaac accaacgcca gaaagaccac caaccccatc accgtggccg
     3481 ttcccctgcc cacccataag accctggaga acaatttctt cgccgtctcc atctgtacca
     3541 gaactccttc cacctcgaag ttcagagtca ggaaccagga cctgtttgat gaacggaccc
     3601 caactccaga ttcctcaggc tgccgacatc tcgcgcttgc ccgtgcggcg cctggcctcc
     3661 tcgaacacgc ttctcgggtc ctggagccag gagagatggt ccaggtgtcc gcaccgcggg
     3721 cactccagcg cctctcgtcc atcatcgtcg gagaccaggt tcctgtcctc gagctcgccc
     3781 gtccggccgc agtagcaggt ctccagcatc gcctttcgcc tccttcggcc cttcgcgggg
     3841 ccgcgtgctt accgtcgcag accatcatgc gccggccgga cgttgcctac atcgtgaaaa
     3901 ctacgcaatg ggacgctgat acgtagaata ggtactctta cctatgaggt aggaaccagg
     3961 aatcggggcc cgtaaccgac gggttcataa ctcctgattc ctacttcaag attcccctct
     4021 cggtggcctc gtgggtggcc gcggttctgg atatgacgcc gagcttgcgg tagacgctgc
     4081 tcaggtgttg tcctacggtc ctggggctga ggtagagcct ctgcgccacc tcctggtcgg
     4141 tgagcccctc ggcgaccagc ccgagcacct cggtctcgcg ggcgctcaat acgccctgcg
     4201 aggcgagagt tggcggggcg acgtccggct cgagcgccag ggagacggcc gcttccagct
     4261 tcatcgacct gccttcggcc cgcagcaacg agaactcccc atcgccgacc ctggtacgga
     4321 cggcggccag ggcgcggtcg tactcggggt tttcgcaggg cggaacgggg acgccggtgc
     4381 tctctctgag cgcttccgcc gcgccgaaca gccgcgccga gcgcccgaag tgcttgctcg
     4441 cggcggccgc ttcggcgagg gcctcaagca cgctggccac acgccccagg tcgccgagat
     4501 cctcgtgtac ggcgaggctc tcccggagat accaacccgc ctgttcgagg tcgcccctgt
     4561 ggtaggcaac catccccagc tggttgagag cccagccgac accctccctg tagccaccct
     4621 ccctggagat ggcgaggctc tcatctagca aggtttccgc acggtcgtag tcttcctcgt
     4681 agagcgcgac ggaccccagg ttcaagaggg aagagaggat ccccgccgcg tcgccggagt
     4741 cgcgggtcat gtccagcgcc ctcgcgctca gatccccggc ccgctcgttc tccccgttta
     4801 gccaggcggc gaggccgaga tagtacagcg actcagcgat gccgtgctcg tcttcgagct
     4861 cgcgccacag ggcgaggctc tcctcgtgca gctttctggc acgtccgtaa tctccctgct
     4921 cgcgcgcgac accaccaagc acatccttca cgctggccac cccgcaccga tcggccagat
     4981 ctttgtacag ggagaggctg tgtcccaggt gctccccggc cccggtgtag tcgcactgca
     5041 agaaagccag gagcccggct cccctcagcg ccccagcccg cagcgaggtg ggatccccgc
     5101 tcgtcgcgag cgcgtcttcg agccatgcac gccccgtctc gtagtagcca cgcgcgtggc
     5161 agaaccccca caacgcgctc gccagccgca ggcccgcctc cgtatcttcg gccgtccgga
     5221 accaggacat agccgccctg aggttgccaa cctccgcgtc cagacgatct atccacgcgc
     5281 cctgatccgg cccagagaga gcgggttcgg cccgctcggc cagccgcagg tagaagcccg
     5341 cgtgcctgct gcgcacggac ctctcctccc ccgaaccctc gagtttcctc gaggcgtact
     5401 gccccaccga ctcgagcata cggtaacgtg cgttctcgcc acgcgtctct acgagaacca
     5461 gcgacttgtc caccagcctg ccgaggagct cgaggacctc accccgctcc acgccgcctt
     5521 cggaacatac ctcctccgcg gcctccaggg taaagccgtt cgagaacgcc gagatcctgc
     5581 ggaacaacgc acgctctctc tccgagagca gctcgtggcc ccagtccatg gtcgccagga
     5641 gggttctctg acggggcagg gcgacccggc ttccgtccgt gagcaacagg aaacggtcgt
     5701 cgaggcgggc ggcgatctgg gacgccgaga gtacccggac cttgaccgcc gccagctcta
     5761 tggcgagcgg tatgccgtcg agccgctggc agatgcgcgc cacggcgggg gcgttgtgtt
     5821 cgtccagttg gaagcccgga accgcggccg cggcgcgctc cacgaacaga cggacggcct
     5881 cgtggcgcgt gagtcgttcg gggacgatct cctcccgcac atccggaagc gagagggagg
     5941 gaacgagcca ggccctctcg cctgggatgc gcagggcctc cctgctggtc gcgaggatct
     6001 tgagcccggg gcacgagcgg aggagctcgt cggccagccc cgcgcacgcc tcgataaggt
     6061 gctcgcagtt gtccagaacc aggagagtgg cccttgactc caggtagtcc agcagcgccc
     6121 cggccggcga gacgccggcc atgcgcaggc cgagcgcccg tgccacaccc tcggccacga
     6181 gctcggggtt cgagagggtg gccagctcga cgaaaacgac gccgtcctcg aagtcttcgg
     6241 ccaggtgggc cgcggcttgc agcgcgagcc gggtcttgcc gcacccccca ggaccggtta
     6301 gcgtcagcag cctgcccgac gccagcatgt gccccaggta gtctagctcg cgctctcgcc
     6361 cgacgaagct gctggcctgc gccggcaagt cggcccgcgg gcgggctcgt acccgccctt
     6421 cccgtagatc cgtaaccgcc atctaagcct cgctaaccat atccaaagcc gaagacccgg
     6481 agcccacggc cagcgcacca gacatactag gctcagaaaa agcaccgcac atcacgcaaa
     6541 tgggtgatct ttgcgagcgg tcagccatca gctttcagct atcggctacg agttggagcc
     6601 agcgacgacc cggcgaaaac gctaccactt gccgaatggt gttgcgttcc acggcgcaag
     6661 aaacgccctc aggtaagaag ctgaaggctg accgctgata gccaggtttc gagggaacct
     6721 cgaaaccgcc ctatctgatc tcgacgaggc cgtggcgggc ggcgaagagc agggcctgta
     6781 tgcgtgagtg cacgccgagc ttgttcaaaa tgttcatcat gtgggtgcgt tctgtgtcga
     6841 cgctcatgtg gagtttggcc gcgatctcct tgttggagag cccttcggcc agggagttca
     6901 ggacctcccg ttcgcggggt gtgatctgcc cgatgctcgc gcgcgcttcg agttcttcct
     6961 cgcggctctg gccggcgagc cgcagaagct cgaccagctc ctgctgggag atgagaggtt
     7021 ctccatcgcc gaggcgcctc gtggcgtcca gtatgtcgtc gacgtcggcc gacttgtgaa
     7081 ggacgctggc cgcgccggcc tcgatggccc tggcgtgatc tgccctgtcc aggctcgccg
     7141 tgaggaccag cgctgcgaaa tcggggttgg cctcacgcaa ctcggcgatg agctccgtac
     7201 cggcgccgtc gggtagggtg aggtctatga ccccgaggtc ggccacctcg gagccgtcca
     7261 tgacggcgcg ggcttcatct agcgtcccgg cctgggcgac cacctggaag tcgggctgct
     7321 ggtcaaagac gtacgcgagc gtctggcgga acgaggcatg gtcctctacg agcaagatgc
     7381 tatgcatgtc ctccctttcg cggacaccgg tacgccctcg taacaggaat ataccagctt
     7441 tggcacctac gggggttctc tccgtcacgt gctcgagagc tctccggcct ccccgccggt
     7501 cagggcggcg aggctcaccc ggagaaagac cctggtgcca acgccctccc tgccctcgac
     7561 ctcgagcttg ccgccgagcg ccgcgacgcg ctcgcgcatc ccagtcaggc ccatcccgaa
     7621 cgcggtctgc ggcccgaagc ccccgccgtc gtcctccacc tccaccagga tctcatctcc
     7681 cgccgcaccg agggttacca ccgcgtgttc ggcgccggag tggcgcctga cgttggccag
     7741 ggcctcctgg acgacgcgca ccacctccac gcccgattcc ccggtgatcc ccgccgggaa
     7801 cccgtcgtcc acgatcagct cgaactcgca accaccgccg atctggcggt tcagctggac
     7861 tatggaggcg acggcgcgga tcacggaacg ctccctgatg ctctcgaggc gaaggtcgta
     7921 gatggcctcg cgcagccccc cgacggccct cttcattgcc tctacctgct ggtccgcctc
     7981 gcgggcacga tttgcgcctt ccggcatcct cctcgagacc tgcacggatt gcagggcgta
     8041 ggtcaggtcc tggagtacca catcgtgcag ctcgcgggct atgcggcggc gctcggcctc
     8101 cctgatctcg cgcagggcct cctcggcccg ctttcgctcg gtgatgtcct cgacggtccc
     8161 ctcgtagcca acgacctcgc cctcatcgcc gtagacggcg cgggcgttgg ccgagatcca
     8221 cgccggggtc ccgtccttgc ggagcacctg cgtctcgaaa ccgctcacca ggccgtccct
     8281 taccacaacc ttggtgaact ccttcctgcg ttccggctcg acatatagct ggtccccgat
     8341 gctggagatg gccccgagca attcctccga agaatcgtag ccgaatatgc gggccatagc
     8401 ggggttcgcc gttatgaacc gcccctcgac ggtggtctgg aagatgccct cgacggcgtt
     8461 ctcgaagatg gaccggtact tcttctcggc ctgccggcgc tcctcctcgg ccaccctctg
     8521 ctcggtgatg tcctggtgga
//
LOCUS       DBNGVD010000021         4873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_185211, whole genome shotgun sequence.
ACCESSION   DBNGVD010000021 DBNGVD010000000
VERSION     DBNGVD010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_185211"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16CM_00570"
     CDS             complement(<1..303)
                     /locus_tag="AC16CM_00570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138348.1"
                     /translation="MYRRELREQNRSSWNAAVGAHDSHRANLPGFLRGGGSTLFPEEV
                     GLLGELRGKTLLHLQCNSGGDSVSLAGLGARVTGIDISDEAIASALKLAGQTGARVR"
     gene            complement(313..1335)
                     /locus_tag="AC16CM_00575"
     CDS             complement(313..1335)
                     /locus_tag="AC16CM_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3138349.1"
                     /translation="MGDTGRKTGGYLYNARVISGLRDRGMAVEEVVPCGASPGEQRAA
                     RRFAFDPSVFDAVVVDALARIVAAPRLDLWRSIRPVVALVHELPGVAGGDPDHERAYE
                     GPLLRADRLVAVSGHGRSVLLDRGVPAERISVVPPGFDGVVAARVSYSGGPPLRALCV
                     AQWIERKGILDLVEAWTLRGRPGAVLELVGETNADPAYAARVREAVQSAPHGSIVVSG
                     RVDDAGLGAAYARADLFALPSRYEGYGIVYAEALASGLPVIACGVGPVPELVGREAAV
                     LVEPGDADGLSAALDLLLGDAALRTRMSEAAIRRASGLPRWEDTVAGLEAVLKGILRT
                     AGVGAR"
     gene            complement(1367..1744)
                     /locus_tag="AC16CM_00580"
     CDS             complement(1367..1744)
                     /locus_tag="AC16CM_00580"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3138350.1"
                     /translation="MYEVYVAARFEAAHRLVGDFGPASRMHGHTYRMEAILRGQRLQQ
                     DGTLYDIGALRQDVDDLAASLDYRDLNEVPGLADVNTTAEAVADYCWERLAAPLRDLG
                     LHSLVVRIWESPDVYAAREDTLG"
     gene            complement(1737..2795)
                     /locus_tag="AC16CM_00585"
     CDS             complement(1737..2795)
                     /locus_tag="AC16CM_00585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3138351.1"
                     /translation="MPSETFPEGNLRARALWFAAPRTAELREETVPPPGTGEVRVEAT
                     ASAVSAGTELLVYRGEVPKGLPLDLPTLEGSYAFPIKYGYATVGRVLDTGSDVEHLDT
                     GDPVFVHHPHQEVFVVPARMPVRLPDGPEPTLGVFCANLETALNIVHDTPLRLGETAL
                     VLGGGVVGLLVARLLKLGGAGVVLVVDPLEKRRGLALAAGADDAFGPEAVEGRVAEAT
                     SGRGADVAIETSGSGAALQAGIDAVATEGTVVVASWYGTKPVDLYLGERFHRGRVRLR
                     SSQVGRLNPEMGPRWDTTRRMETVLALLERVEPGDLITHRLPFHRAPEAYPLLEAGTE
                     EAVQVVFTYERIREGRDV"
     gene            complement(2785..3471)
                     /locus_tag="AC16CM_00590"
     CDS             complement(2785..3471)
                     /locus_tag="AC16CM_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR3138352.1"
                     /translation="MGEAALARPTVTVTYAQTLDGRVATATGESKWISCPDSLRFAHE
                     MRAGHDAVLVGAGTVCKDDPRLTVRHVPGDDPLRVVVDSTLRTPPQAAVLADDAARGT
                     VLAATDLAPARRRERALSSGATVLELPADADGRVDLGALMERLRALGVGSVMVEGGAT
                     LITSFLRAGLVDRLAVCLAPKILGNGIQAVGELGIRDLSRSLTLADASVEPYGVDLVL
                     SGRVVYPDAV"
     gene            complement(3495..4145)
                     /gene="ribA"
                     /locus_tag="AC16CM_00595"
     CDS             complement(3495..4145)
                     /gene="ribA"
                     /locus_tag="AC16CM_00595"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3138353.1"
                     /translation="MHSIRRVARAGIPTQHGDFEMFVYEAADRKEHVALFTGFEPGTP
                     DEKEPPLVRLHSECLTGDTFGSLRCDCGEQLEESMRLLHEQGRGVLLYLRQEGRGIGL
                     ANKISAYALQDEGLDTVQANLALGLPDDARDYRVAAEMLLDLGVRRAAVLTNNPAKIE
                     GLRRHGVEVVERLPVRVEPNPFNVSYLQTKREKLGHLIPSPFSPGGRAEGSTNGSS"
     gene            4264..4659
                     /gene="folB"
                     /locus_tag="AC16CM_00600"
     CDS             4264..4659
                     /gene="folB"
                     /locus_tag="AC16CM_00600"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3138354.1"
                     /translation="MDREPVGEDRILLTEMVFHGRHGTLPAENELGQPFVVSIELYLD
                     LRPAGSSDDLTRTVDYSTVHRLARDIVEGPPVQLVETLAERIAAGVLADHPTVEAVRV
                     KVAKPHVRLGDTVLAGSAVEILRRRQDVV"
BASE COUNT          801 a         1704 c         1585 g          783 t
ORIGIN      
        1 acgtacccgg gcgccggtct gcccggcgag ctttagggcg gaggctattg cctcatcgct
       61 gatatctatg ccggtgaccc tcgcgccaag cccggcgagg cttaccgaat cgccccccga
      121 gttgcactgg agatggagga gggtcttgcc ccgcagctcc ccgaggaggc cgacctcctc
      181 ggggaacagc gtgctgccac cgccgcggag gaagccgggg aggttcgcgc ggtggctgtc
      241 gtgggccccg accgcggcgt tccacgacga ccggttctgc tcgcgaagct ctctacggta
      301 cacgggtaga ttctacctcg cccccactcc ggcggtacgc aggatgccct tcaggacagc
      361 ttccaggccc gcgaccgtgt cttcccatcg gggcaggccg gaggcgcgtc ggatcgccgc
      421 ttcggacatc ctcgtcctca aagcggcgtc cccgagcagg agatccaggg ccgcactcag
      481 gccgtcggcg tcgccgggct cgaccaggac cgcggcctcc cttccaacta gctcgggaac
      541 gggtcccaca ccgcaggcta tgacgggcag accggacgcg agcgcctcgg cgtagacgat
      601 cccgtatccc tcataacggg agggcagagc gaagaggtca gccctcgcgt aggcggcacc
      661 aagcccggca tcgtcaactc gtcccgatac tacgatggag ccgtgcgggg ccgactggac
      721 tgcctcccgg actcttgcag cataggcggg atctgcgttc gtctcgccga cgagctcgag
      781 taccgcgcca ggacgcccgc gcagcgtcca cgcttcgacc aggtcgagta tgcctttgcg
      841 ctcgatccac tgcgcgacgc aaagggcgcg caagggagga ccgccgctgt aagagactct
      901 ggccgccaca acgccgtcga agccgggcgg cactacgctg atgcgttccg cagggacgcc
      961 ccggtccagg agcacgctcc gcccgtggcc actcacggcg acgagccgat ctgcccgcag
     1021 caggggcccc tcgtacgctc gctcatggtc ggggtcgccg cctgcgacac cgggcagctc
     1081 atgtaccagc gccaccacgg gccggatgga ccgccagaga tccaggcgcg gcgcggccac
     1141 gatacgggcc agggcgtcga ccactaccgc gtcgaatacg gaggggtcga aagcaaaccg
     1201 tcgggcggcc cgttgctccc ctggggaggc accgcaaggt accacctcct cgaccgccat
     1261 acccctgtct cgaagccccg agatcacccg cgcgttgtag agatagccgc ctgtcttgcg
     1321 gcccgtgtcc cccaccgtga cgaacgcgac gcgcagggcg acttccctag cccagggtgt
     1381 cttcgcgggc ggcgtagacg tcgggggatt cccagatgcg taccactagc gagtggagac
     1441 ccaggtcgcg caggggcgcg gctagcctct cccagcagta atccgccaca gcctcggcgg
     1501 tggtgttgac gtcagcgagg ccaggtactt cgttcagatc ccgatagtca agggaggctg
     1561 caaggtcgtc aacgtcctgc ctcagcgcac cgatatcgta gagtgtgccg tcttgctgaa
     1621 gacgctggcc gcgcaggatc gcctccatcc tgtaggtgtg accgtgcatc ctgctcgccg
     1681 gcccgaagtc tccgacgagc ctgtgcgccg cctcgaacct tgccgcgacg tagacctcat
     1741 acatcccgtc cctcccggat acgttcgtag gtgaaaacta cctgaaccgc ctcttctgtt
     1801 cccgcctcga ggagcgggta cgcctccggg gccctgtgaa acggaaggcg gtgggttatg
     1861 aggtcgccag gttccaccct ctcgagaagg gcaaggaccg tctccatgcg cctggtcgtg
     1921 tcccagcgcg gccccatctc ggggttcaac cgcccgacct gtgaggagcg caacctcacc
     1981 ctgcccctgt ggaatcgttc cccgaggtac agatcaacgg gctttgtgcc gtaccatgag
     2041 gccacgacga ccgtgccctc tgtcgccacg gcgtcgatcc cggcctgcag ggcggccccg
     2101 gacccgctgg tctcgatggc gacgtcggct ccccgcccgc tggtggcctc cgcgacacgc
     2161 ccttctaccg cctcggggcc gaacgcgtcg tcagccccgg cagcaagcgc cagcccgcgg
     2221 cgtttttcca gcgggtcaac gacaagcacc actcctgcgc cgcccagctt cagcagccgg
     2281 gctaccagga gacccacgac gcctcccccc aggacgagcg ccgtctcgcc gaggcgcagg
     2341 ggcgtgtcgt ggacgatgtt aagggccgtc tcaaggttgg cgcagaacac gccgagggtg
     2401 ggttcggggc cgtctgggag ccgcacgggc attcgcgcgg gaaccacgaa aacctcctgg
     2461 tgcgggtggt gaacgaagac cgggtctccc gtatcgaggt gctcgacgtc ggatcccgtg
     2521 tccagaaccc gacccaccgt ggcgtaaccg tacttgatgg ggaacgcgta gctgccttcc
     2581 agggtcggca ggtccagggg gaggcccttc ggcacctcgc cgcggtagac gagcaactcc
     2641 gtgccggcgc taacggccga tgccgtagcc tcgacgcgca cctcgcccgt tccgggtggc
     2701 ggcacggtct cctcgcgtag ctccgcggta cgcggggctg cgaaccagag ggctctggcc
     2761 ctcaggttcc cttcggggaa cgtctcagac ggcatcggga tagactacgc gtccgctcag
     2821 gacgaggtcg acgccgtaag gttcaaccga ggcgtcggcc agggtgaggg agcgggacag
     2881 gtcccgtatg cccagttcgc cgacggcctg aatgccgttg ccgagaatct tgggcgccaa
     2941 gcagacggcc agccggtcca ccagcccggc tcttagaaag ctcgtgatca gggtcgcccc
     3001 accctccacc atcaccgacc ccacaccgag cgccctgagt cgctccatca gggccccgag
     3061 gtcgacacgc ccatcggcat cggccggcaa ctccagaacg gtggccccgg aggatagagc
     3121 cctctcacgc cgtctcgcgg gcgcgagatc cgtagccgcc agcacggtcc cccgggccgc
     3181 gtcgtcggcc agaactgcgg cctgcggcgg cgtacgcagg gtactatcca cgaccacgcg
     3241 cagggggtcg tcccctggca cgtggcgtac ggttagtcgg gggtcgtcct tgcatacggt
     3301 gccggcgcct acgagaaccg cgtcgtgtcc cgcgcgcatc tcgtgggcga agcgcaggga
     3361 gtcggggcag ctgatccact tcgattcccc cgtcgcggtc gccacgcgcc cgtcgagcgt
     3421 ctgggcgtag gtgacggtga cggtaggccg cgccagggcc gcctcgccca ccgaaggctc
     3481 ttgctcctcg cctatcatga tgacccgttc gtcgagccct cggcgcggcc tccgggagag
     3541 aacggtgagg ggatgaggtg gcccagtttc tccctcttcg tctgcaggta ggagacgttg
     3601 aaggggtttg gttcgaccct taccggcagg cgctccacga cctcgacgcc gtggcgcctg
     3661 aggccctcga tcttggccgg gttgttcgtg agcacggcag cgcggcgcac cccgaggtcg
     3721 aggagcatct cggcggcgac gcggtagtct cgcgcgtcat cgggcaaccc gagggccagg
     3781 ttagcctgca cggtgtcgag gccctcgtcc tggagcgcgt aggcggagat cttgttggcg
     3841 agccctatac cgcgtccttc ctgcctgagg tagaggagga cgccgcgccc ctgctcatgc
     3901 agcagccgca tgctctcctc aagctgctca ccgcaatcgc agcgcaggga cccgaaggtg
     3961 tcacccgtaa ggcattcgga atgcagacgg acgagcgggg gttccttctc gtcaggtgtg
     4021 cccggctcga accccgtgaa gagggccacg tgctccttgc gatccgcggc ctcgtagacg
     4081 aacatctcga agtcaccgtg ttgggtcggt ataccggccc gtgcaacgcg tcggatagaa
     4141 tgcatggttc ccgtttcccg atctctaggt catggccacc tggcccgaga ttctactaca
     4201 gccttcctca gcaccaaccg cgtcttcagc gtttggctac cccgatctgg catactgttc
     4261 tccatggata gagagcctgt gggcgaagac cgcatactgc tcaccgagat ggtctttcat
     4321 gggcgtcacg gcaccctccc ggccgagaac gagctcggcc agcccttcgt cgtcagcatc
     4381 gagctgtacc tcgatctgcg gccagcggga tcctccgacg acctgaccag gaccgtggac
     4441 tacagcacgg tacaccgcct ggcgcgcgat atcgtcgagg gaccgcccgt gcaactcgtc
     4501 gagaccctgg ccgagaggat cgccgctggc gtcctggctg accaccccac ggtcgaggcg
     4561 gtcagggtaa aggttgccaa gccccacgtc aggctcggcg acaccgtgct ggcaggctcg
     4621 gccgtcgaga tcctgcgccg ccgccaggac gttgtctagg cccctgaact agatacgagt
     4681 cgcgatccac ccgagaactt agaaccacct cggtcggttc tgcgtactaa catgtgaagt
     4741 ggaggcctcc tgggcttcgg gtggagagac agaaaggacg gcacccctgg tgacggacag
     4801 gctcgagaac gccgctccgc ccaacgccgc agttctcgcc ccggaaagtg ttctggagag
     4861 cgtcgccccc ctc
//
LOCUS       DBNGVD010000022         3737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1278486, whole genome shotgun sequence.
ACCESSION   DBNGVD010000022 DBNGVD010000000
VERSION     DBNGVD010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1278486"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="AC16CM_00605"
     CDS             complement(<1..584)
                     /locus_tag="AC16CM_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3138355.1"
                     /translation="MVVGSGGREHALVEALAASPMEPAMYAAPGNPGISRIAETVDIP
                     VDDIVGLRDYAATNSIDLTVVGPEVPLIGGITEAFWEAGLKVFGPSRAAARIEGSKVF
                     AKELMRHAGVPTAQFEAFDRQEPALDYLRSRSEYPIVVKADGAAAGKGVTVARSQDEA
                     VGAVVAAFAGKFGAAGERVVIEECLEGREASIFVV"
     gene            complement(612..1898)
                     /locus_tag="AC16CM_00610"
     CDS             complement(612..1898)
                     /locus_tag="AC16CM_00610"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3138356.1"
                     /translation="MTATVVLGLAWGDEGKGRVCDALASDVGYVSRYSGGNNAGHTVR
                     VGEEEFKLHLVPSGIVREGVVCTIGNGVVVNPEVLVEEVEALEARGLRVRDRLKVDGR
                     AHLIMPYHIALDSHREIALGEARIGTTNRGIGPAYEDKVARSGIRVQDIFDEGILKTK
                     LKAALREKNAIFENVYGEEHYDVSEVATWLLSFQDFIGPMVADTGTLLRGALENRERV
                     LLEGGQATLLDNDHGTYPFVTSSNPTVGGAVVGSGIPARQLEYVVGVTKAYTTRVGDG
                     PMPTELFDETGETIRSVGREYGTTTGRSRRVGWLDLPAVKWAAALNGITHVAITLLDV
                     LSAVDEINICVGYEIDGKPVEGYPMNQTDLHHARPIYKTLPGWGEDITWCRMRGDLPE
                     AARDFVGFVEAEVGAPLCMISVGPEREQAIVERIGI"
     gene            complement(2167..3495)
                     /locus_tag="AC16CM_00615"
     CDS             complement(2167..3495)
                     /locus_tag="AC16CM_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3138357.1"
                     /translation="MPEMGKTASTGRGMSRKRFLGLGGASLAGMAMLGATGCGGGGEA
                     GGPVGITFTFGPEASGSLQGLIDKFNKEHKGEIEVTWRTTAAASDDYFEQIKTELQAG
                     KATVDVIGGDVIWPAQLAAQGYVLDLSDRVTPEMKAKNLAGPVKATQYDGKTWAVPWF
                     TDAGMFYYRKDLLQKSGFSDPPGTWGEMKRMAEMVRSDSGTRFGYVFQGAQDEGGVCD
                     GLEHVWNAGGDVLDGDRVIVDSPESVEGLDLRRSMITDGLSPDASVNYTTQESQSIFT
                     NGDAVFMRNWTFVYGLLASPETSRIKPDQVGISTLPVTEAGQESSSALGGWNFLVNAA
                     SEDKLDEVWTFIEYMSSPESQRTFALQSARLPTLTSLYEDEEVQRKVPVAALGKAALE
                     KARPRPISPYYSDMSLVMAERFNEALKGSSPVDRALEEMQADLQNIVEQT"
BASE COUNT          595 a         1316 c         1121 g          705 t
ORIGIN      
        1 acgacgaaga tggaggcctc ccttccctcc aggcactcct cgatcacgac cctctccccc
       61 gccgccccga acttgccggc gaacgcggcc actacggctc ctacggcctc gtcctgtgag
      121 cgtgccacgg tgacgccctt gccggcagcc gccccgtcgg ccttcacgac gatggggtac
      181 tcggaccgcg acctgaggta gtcgagtgcc ggctcctggc ggtcgaaggc ctcgaactgg
      241 gccgtcggga cgccggcgtg gcgcatgagc tccttggcga aaaccttgga gccctcgatg
      301 cgggcggcgg cccgcgatgg cccgaaaacc ttgaggccgg cctcccagaa cgcctcggtg
      361 atccccccga taagcggcac ctcgggcccg accacggtca ggtctatgct gttggtggcg
      421 gcgtaatccc ggaggccgac tatatcgtcg accggtatgt ccaccgtctc tgctatgcga
      481 gagatgccgg ggtttccggg ggccgcgtac atggcgggct ccatcgggct cgcggcgagg
      541 gcctcgacca gcgcgtgctc gcgcccccca cttcctacca ccatcacctt caagcgtcgt
      601 gccccacggt cctagatccc tatcctctcc acgatagcct gctcgcgctc gggaccgacg
      661 ctgatcatgc acagcggagc cccgacctcg gcctcgacga accccacgaa atcgcgcgcg
      721 gcctctggca ggtcccccct catcctgcac cacgtgatgt cctcgcccca ccccggcaac
      781 gtcttgtaga tgggcctggc gtggtgcagg tccgtctggt tcatgggata accctccacg
      841 ggcttgccgt cgatctcgta cccgacgcag atgtttatct catcgaccgc ggagagcacg
      901 tcgagcagcg tgatggcgac gtgcgtgatg ccgttgagcg cggcggccca cttcacggcc
      961 ggcaggtcga gccatccgac cctccgcgac cgtcccgtag tcgttccgta ctcccgaccg
     1021 acgctcctga tggtctcccc cgtctcgtcg aagagctccg tgggcatcgg gccgtcgccg
     1081 accctggtgg tgtaggcctt ggttaccccg accacatact ccagttgacg cgcggggatg
     1141 ccggagccaa ccacggcccc cccgacggtc gggttggaag aggtcacgaa gggataggtg
     1201 ccgtggtcgt tgtccaggag cgtcgcctgc cccccttcga gcaggacgcg ctccctgttc
     1261 tcaagcgccc cccgcaggag ggtcccagta tcggcgacca tcggcccgat gaagtcctgg
     1321 aaggagagca gccaggtggc cacctccgag acgtcgtagt gctcttcgcc gtagacgttc
     1381 tcgaagattg cgttcttctc cctgagggcc gcctttagct tggtcttcag gatgccctcg
     1441 tcgaagatgt cctggacgcg tatgccggag cgggcgacct tgtcctcgta ggccgggcct
     1501 atgcccctgt tcgtcgtccc tatcctggcc tcgccgaggg cgatctcccg gtgcgagtcg
     1561 agcgcgatgt ggtagggcat gatgaggtga gcacggccgt ccaccttgag gcggtccctg
     1621 actcgtaagc cccttgcctc cagcgcctcg acctcctcga ccagcacctc cgggttgaca
     1681 acgaccccgt tgccgatggt gcagaccacc ccctcgcgca cgatgccaga gggaacgagg
     1741 tgcagcttga actcctcctc gccgaccctg accgtgtggc ccgcgttgtt gccgccggag
     1801 tatcgcgaca cgtacccgac atccgacgcc agcgcgtcac aaaccctgcc cttgccctca
     1861 tctccccagg ccagcccgag cacgaccgtg gccgtcaaag ctccacctcc gcggcgatat
     1921 ggatggttcc aaaacgagcg tggcgatacg taaaacggag tagcccccac cggggaccga
     1981 aactcaccgt ccgccgatcg gggccgccgc tctctaccac ggtggcagcg tagcacatag
     2041 gtgtatggtt ttgaatcgcc cgaagcggat ttcgggaact tcccgaaatc cgcgccgtca
     2101 gctcttggca atcagcggtc agccaagaac aaaaagctga tggctgaccg ctgattgctg
     2161 acggctctat gtctgctcga cgatgttctg caggtcggct tgcatctctt cgagggcccg
     2221 gtccaccggc gagctacctt tcagggcctc gttgaagcgt tcggccatca ccaacgacat
     2281 gtcagagtag tagggtgaga tggggcgtgg cctcgctttc tccagggcgg ctttgccgag
     2341 ggccgccacg gggacctttc gctggacctc ctcgtcttcg tagagggatg tcagggtggg
     2401 taggcgtgcg ctctgcaggg cgaacgttct ctgggactca ggtgaggaca tatactcgat
     2461 gaaggtccag acctcgtcca gcttgtcctc tgaggccgcg ttgaccagaa agttccatcc
     2521 gcccagggcc gaggagctct cctggcccgc ctcggtaacc ggcagggtcg agatgccgac
     2581 ctgatccggc tttatccgcg aggtctcggg gctcgcgagc aacccgtaga cgaacgtcca
     2641 gttgcgcata aagaccgcat cgccgttggt aaagatggac tgcgattcct gggtggtgta
     2701 gttcacgctc gcgtcgggag agaggccgtc cgtgatcatg gatctcctga gatcgaggcc
     2761 ctcgaccgac tcggggctat ccacgatgac cctgtcgccg tctagcacgt cccctccggc
     2821 gttccaaacg tgctcgaggc cgtcgcatac cccgccctcg tcctgcgccc cctggaacac
     2881 gtagccgaac ctggtccccg agtccgacct gaccatctcg gccatccgct tcatctcgcc
     2941 ccaggtccct gggggatctg agaagccgct cttctggagc aggtccttcc tgtagtagaa
     3001 catcccggcg tcggtgaacc acggaacggc ccacgtcttg ccgtcgtact gcgtggcctt
     3061 cacgggaccc gccagattct tcgccttcat ctcgggcgtg acgcggtccg agaggtccag
     3121 gacgtagccc tgcgcggcga gctgggcggg ccagatcacg tccccgccga tcacgtccac
     3181 cgtggccttc ccggcctgaa gttcggtctt tatctgctcg aagtagtcgt cgctcgcggc
     3241 ggcggtggtc cgccacgtga cttctatctc gcccttgtgc tccttgttga acttgtctat
     3301 gaggccctgc aagctccccg aggcctccgg cccgaaggtg aaggtgatcc ccaccgggcc
     3361 gcccgcttct ccaccgccac cgcagccggt ggcccctagc atcgccatcc ccgccagcga
     3421 ggccccaccc aacccgagga acctcttccg gctcatgccg cgtccggtgg atgcggtctt
     3481 ccccatctca ggcatagtcc agccctttct cgtctacccg ctgccaacat agaccagaag
     3541 gcgcccgccc gcagatcggt gcgggtgaac gcgcaacaga cttttcaact cgcattacag
     3601 aggggtcgtg cctggcccgc ccgcaacaga gggccggcgg gccgaggtgt tgaccggcta
     3661 gcttacgctt tcttcgcggc gtcgacccag ggcttcacga ggtccttgtt cttcatgtac
     3721 cacttcttga cgccctt
//
LOCUS       DBNGVD010000023         2914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3385, whole genome shotgun sequence.
ACCESSION   DBNGVD010000023 DBNGVD010000000
VERSION     DBNGVD010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3385"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..721)
                     /locus_tag="AC16CM_00620"
     CDS             complement(101..721)
                     /locus_tag="AC16CM_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138358.1"
                     /translation="MTDQDKTRDVNEAARRFAETLADSYRLVYGQATESAERQQQRAQ
                     EFSDLVQGNLQEQAEAGRANMGQLTEQANKQREAGQQLARESVEAYTQFLNDAFSQYR
                     AGADEAAGTVRESARTLSDTATGLVGTATGMVGSAAESQVDAANAAAESAVFPISGYD
                     DMNVDEISKELNDLSADELRLVRDYEERNKNRASLLEQMDRKIRSE"
     gene            890..1606
                     /locus_tag="AC16CM_00625"
     CDS             890..1606
                     /locus_tag="AC16CM_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3138359.1"
                     /translation="MITSTAEILIVEDDEVIMGTLAYNLSRNGFGVSQATNGSDALRM
                     ARKLRPDLILLDIMLPGESGVEVCERIRDRDQGVMIVMITAKDSEEDKVRGFEAGADD
                     YVTKPFGMKELVARINANLKRSETGARGKVIEAGDLSIDTKNFTAFAGGESLELRLKE
                     FELLAALASTPGELRSREELAKEVWGHAGVGSSRTIDVHIRRIRAALEQRSAFEYVHT
                     ARGLGYRFEPKQRPLLEDGE"
     gene            1652..2353
                     /locus_tag="AC16CM_00630"
     CDS             1652..2353
                     /locus_tag="AC16CM_00630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3138360.1"
                     /translation="MQRVNKIRRDFVANVSHELKTPATSLILLAESLEQTLDEDPVQA
                     RLFAAQLRQETERLAHLITDLLDLTRLESQEPTDYPTLVDVRGILMTVLARMRRVARR
                     KDITLQWKRFGTAASYTVYGDETQLTSMFTNLVDNAVQYTPPGGRVEVVGGSESSEII
                     ISISDTGIGIPEDKIARIFERFYRVDRARSKATGGTGLGLSIARHVAQNHGGRVAVES
                     IPGEGSTFTVYLPRG"
     gene            2567..>2914
                     /locus_tag="AC16CM_00635"
     CDS             2567..>2914
                     /locus_tag="AC16CM_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3138361.1"
                     /translation="MGIGRSDTGQSGDPGYRGLSDATFEGIALSEGGRILEVNSSFVE
                     MFGYGASEVVGMPAVDFVAPESREAVAEHIAMASPEPYEAVALRKDGTVFDIEVRGKS
                     SEYQGRVVRVTAIR"
BASE COUNT          508 a          884 c          999 g          523 t
ORIGIN      
        1 ctggtcgcca gacggcccac tccctgccac gcgtacctcc tcctgggcta tcagcggtca
       61 gctttacttg ctgaccgctg atagctgacg gctgaaagct ctactcggac ctgatctttc
      121 ggtccatctg ctcgaggagc gaggcgcggt tcttgttgcg ctcctcgtag tcgcgcacca
      181 gacggagctc gtccgcggag aggtcgttca attccttgga aatctcgtct acgttcatgt
      241 cgtcgtagcc ggagatgggg aagacggctg actccgcagc ggcattcgcc gcgtccacct
      301 gggattcggc cgcgctgccg accatgccgg tcgcggtgcc caccagcccg gtggccgtgt
      361 cggagagggt tcgggcgctc tccctgaccg ttcctgccgc ctcgtcggcg ccggcgcggt
      421 actgggagaa ggcgtcgttc aggaactgcg tgtaggcctc gaccgactcg cgggcgagtt
      481 gctggcccgc ctcccgctgc ttgttggcct gctcggtgag ctgccccatg ttggcgcgac
      541 cggcctcggc ctgttcttgc aggttaccct gtaccaggtc ggagaattcc tgggcgcgct
      601 gttgctggcg ctcggccgac tcggttgcct ggccgtagac cagcctgtaa gagtccgcca
      661 gggtctcggc gaagcgccgg gccgcctcgt tgacatcgcg cgttttatcc tgatctgtca
      721 tggtctcctc tcttttactt gtgcgtgctt gttgatccct ttgaggcatt ctacccagat
      781 ggggcctgtg ctacacgcgt ccgttaaagt cccgttaaat cggtgaagcc cggggtggca
      841 gcgcttatac ttgcaatgcc tctggcaggt tccgaagaga gagggcgcat tgataacaag
      901 cacggcggag atcctgatag tcgaggacga cgaggtcatc atgggtaccc tggcctacaa
      961 cctctcccgc aacggcttcg gggtcagcca ggccaccaac ggctccgacg ccctcaggat
     1021 ggcaaggaag ctgaggcctg acctgatcct tctagacatc atgctccccg gagagtccgg
     1081 ggtggaggtg tgcgagagga tccgcgaccg cgaccagggc gtgatgatcg tgatgatcac
     1141 ggccaaggac tccgaagagg acaaggtgcg cggtttcgag gccggggctg acgactacgt
     1201 gacgaagcct ttcggcatga aggagctggt cgcccgcata aacgcgaacc tgaagcgctc
     1261 cgagacgggc gcgaggggca aggtgataga ggctggcgac ctctcgatag acacgaagaa
     1321 ctttacggcc ttcgcgggcg gggagtcgct ggagcttcgc ctcaaggagt tcgagctgct
     1381 ggccgccctt gcctcgaccc ctggcgagct gaggagccgc gaggagctgg cgaaggaggt
     1441 gtgggggcac gcgggcgttg gctcctcacg caccatagac gtgcacatac gcaggatacg
     1501 ggccgccctg gaacagaggt cggccttcga gtacgtccac accgccaggg gcctcggcta
     1561 ccggttcgag ccgaagcaga ggcccctcct ggaagacggg gagtagacgc tggaagagcg
     1621 aaacggtgcc cgtcccgtca gccccgacga gatgcagcgg gtcaacaaga tccgccgcga
     1681 cttcgtcgcg aacgtctccc acgagctgaa gacgccggcg acgagcctca tattgcttgc
     1741 cgagagcctg gaacagaccc tggacgaaga ccccgtccag gcacgcctct tcgccgccca
     1801 gctgaggcag gagaccgagc gtctggccca cctcattacg gacctgctcg acctgacgcg
     1861 cctggagagc caggaaccaa ccgactaccc gaccctggtc gacgtgcggg gcatcctgat
     1921 gaccgtgctt gcccgcatgc gccgcgtcgc ccgccgtaaa gacatcaccc tgcagtggaa
     1981 gcgcttcggc acggccgcca gctataccgt ctacggcgac gagacccagc tcacctctat
     2041 gttcaccaac ctggtggata atgccgtcca gtacacgccg ccggggggcc gggtcgaggt
     2101 ggtcgggggc tccgagagtt ccgagataat cataagcatc tccgacacgg gcatcggcat
     2161 accagaggac aagatcgcac gcatcttcga gcgcttctac cgcgtggaca gggcacgctc
     2221 caaggcgacg ggcggcacgg ggctggggct ctccatcgcg cggcacgtgg cccagaacca
     2281 cggcggccgg gtagccgtag agagcatccc gggcgaaggg tcaacgttca cggtttatct
     2341 gccgcgcggg taacgcgcgg tcgggcgggt ttcaaacacg cgatacgggg tctggggtgt
     2401 cccggggtgc gcctggaggc cgcacctgag ggtgtcaggg gtattagcac cccgtgttga
     2461 ggtcggaggt ataatttccg ttgcgaggga tggtgaggca tcccgacggt cgctggcacc
     2521 gtattcggag ggtggagcgc gccgggcttg tggggctgga ggaagaatgg gtatcgggag
     2581 gtctgatacg gggcagagcg gggacccggg gtatcggggt ctctccgatg cgacgttcga
     2641 gggaatagcc ctgagcgagg gtggcaggat tctcgaggtc aacagctctt tcgtcgagat
     2701 gttcggttac ggggcgtcgg aggtcgtggg catgccggcg gtagatttcg tggcgcccga
     2761 gtcgcgcgag gcggttgcgg agcatatagc gatggcctcg ccggagccgt acgaggccgt
     2821 ggcgctcagg aaagacggga cggtcttcga catcgaagta aggggtaagt cctccgagta
     2881 ccagggccgc gtcgtcaggg tcaccgccat ccgc
//
LOCUS       DBNGVD010000024         3344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_914451, whole genome shotgun sequence.
ACCESSION   DBNGVD010000024 DBNGVD010000000
VERSION     DBNGVD010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_914451"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="AC16CM_00640"
     CDS             complement(<1..730)
                     /locus_tag="AC16CM_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3138362.1"
                     /translation="MADHPAPQLLCLAVLEGLRGRARELGRAARSLGKRSRTLIRVYS
                     FLLGQRERAYLELGRWRSGDAIDPGRMVWIFGSGRSGSTWLRSMMGELPGAGVWEEPM
                     VGRLFGEFYSRAPKDNLRSADFVMGDPIRRGWIRSVRNFVLDGALYSNPRLGSGGYLI
                     IKEPNGSVGAPLLMEALPESRMILLVRDPRDVIASVLDAAAEGGWLYESRGGKTNASR
                     NTDRRPNAFVRERANVYRRGVESAW"
     gene            876..2381
                     /locus_tag="AC16CM_00645"
     CDS             876..2381
                     /locus_tag="AC16CM_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR3138363.1"
                     /translation="MFSVLSVLVVSAVGSGAGADARQTSLADEMDRASGEYGVPGELL
                     LAMGYVNTRWEMPPPSASDYKADDPEGRGNYGVMALVRNPSEDTLGRASALTGESVEA
                     IKNDRASNVRGGAAVLADLAGEEKPQNLDGWYDAVSRYGGGELYAQQVYEVLREGAAA
                     TTSTGERLELAPREVEVPALYSAQATGDYPRSRWYGNGGRNYTESRREPTSDVNTIVI
                     HMTQGSWSSAINYFASSSNESASAHYTVRSSDGFIGQSVRESDIAWHAGWWPTNKHSI
                     GIEHEGYVDQPRWFTDAMYRSSARLSAYLAVKYHIPIDRKHIIEHKQVPGCSTGGGGV
                     GCHTDPGRYWNWRKYMDLVKDRARTIRNNTYQQVVDNATPGRFKASDHWNVSRRHTTT
                     SYGPNQRVLPRPLSVSRNAAFKIRTPARGSYRVYGWWPADRDYNARTVFRIKTTGGWT
                     RRTVNQQINGGRWIYLGTYNLAENDSYRVEVSSKSPGRGRVVADAVKVTRS"
     gene            2399..>3344
                     /locus_tag="AC16CM_00650"
     CDS             2399..>3344
                     /locus_tag="AC16CM_00650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3138364.1"
                     /translation="MSIERGRLLVDRRDFLRIGGTGLAGAVLLGGATTAAFARTGALS
                     KEFGAAAREYRVPEELLLAMGYVNTLWEMPPPSASDYEPGDLSGRGAYGIMQLVQNPW
                     ADTLARAAALTGLSQASLKASRPANIRGGAAVLAQMQGTESPGDLGAWQQAVAEYGGG
                     ALYAEQVYETLKDGASATISTGERLEIAPHEDVEAPATYTAASTPDYKRARWRPAYGG
                     NYTNSNREASYNIGRIVIHTVEGSYSSAISWFQNPRANVSAHYVVSRRGDVAQCVRDA
                     DVAWHAGNWNWNTHSIGIEHEGYSGNRSTWTNAMYHASA"
BASE COUNT          658 a         1091 c         1075 g          520 t
ORIGIN      
        1 gccaggcgct ctccacaccg cgccggtaca cgttcgcccg ctccctgacg aaggcgttcg
       61 gtctcctgtc ggtgttgcgg ctcgcgttcg tcttcccccc cctgctctcg tagagccaac
      121 cgccctccgc ggcggcgtcc aggacggagg cgatcacgtc cctggggtct cgcacgagca
      181 ggatcatgcg gctctcgggc agggcctcca tgagtagcgg agccccgacg gacccgttgg
      241 gctccttgat gatgaggtac cctcctgacc cgagacgggg gttcgagtac agcgccccat
      301 ccagaacgaa gttccttacg gagcgtatcc aacccctcct tatggggtcc cccatcacga
      361 aatccgcgga gcgcaggttg tctttcggcg cccgcgagta gaactccccg aagaggcgcc
      421 cgaccatggg ctcctcccag accccggcgc ctggcagctc ccccatcatg ctgcgcagcc
      481 acgtgctccc gctgcggccc gagccgaaga tccagaccat ccgcccaggg tctatggcat
      541 ccccggacct ccatcgtccc agctcgaggt aggcccgctc ccgctggccg aggaggaaac
      601 tgtagacccg gatcagggtc ctgctcctct tccccaggct acgtgcggcc cgcccgagtt
      661 cccgggcgcg ccccctcagg ccctcaagca cggccaggca tagaagttgg ggtgctggat
      721 ggtcggccaa atgccattct ctggacctcc gtaacgaaga cgtcccgaaa agtgtgtcac
      781 agaaccctta acttttctcg aaacctggca cataatggtg tccgtcacaa ggagcatctc
      841 tctgggggga gaacttgcgt tacaggacgt tggtcttgtt ttctgttcta agcgtgctcg
      901 tcgtatccgc ggtaggctcc ggcgcgggcg ccgatgccag gcagacgtcc cttgcggacg
      961 agatggaccg tgcctctggc gagtacggtg tcccggggga actgcttctg gcgatgggct
     1021 acgtgaacac ccgctgggag atgcccccac catcggcgag cgactacaag gccgacgacc
     1081 cggaaggacg gggcaactac ggggtgatgg ccctggtgag gaacccctct gaggacacgc
     1141 tgggcagggc ctcggccctt accggagaat cggtggaggc gatcaagaac gaccgcgcct
     1201 cgaacgtgag gggtggggcc gcggtgctgg ccgacctggc tggtgaagag aagccacaaa
     1261 acctcgacgg ttggtacgac gccgtctccc gctacggtgg cggtgagctg tacgcgcagc
     1321 aagtctacga ggtcttgagg gagggtgccg ccgcgaccac ctcgacgggc gaacgtctgg
     1381 agctcgcacc gcgcgaggtg gaggtacccg cactctacag cgcccaggcg acaggggatt
     1441 acccgcggtc caggtggtac ggcaacgggg gccgtaatta caccgagtcg aggcgtgagc
     1501 caaccagcga cgtaaacacc atcgtcatcc acatgaccca gggttcctgg tcgagcgcca
     1561 taaactactt cgccagcagt agcaacgaga gcgcttcggc ccattacacc gtcaggtcct
     1621 ccgacggctt tatcgggcag tcggtccgtg agtcggacat agcctggcac gcgggctggt
     1681 ggccgaccaa caagcacagc atcggcatcg agcacgaagg ctacgtggac caaccgaggt
     1741 ggttcacgga cgcgatgtac cgctcgtcgg caaggctctc cgcttacctc gcggtcaaat
     1801 accatattcc gatcgaccga aagcacataa tcgagcacaa gcaggtcccc ggctgttcca
     1861 ccggcggggg cggcgtcggc tgccacaccg atccgggccg gtactggaac tggcgcaagt
     1921 acatggacct cgtaaaggat cgagccagaa ccatcaggaa caacacgtac cagcaggtcg
     1981 tggacaacgc cacgcccggc cgcttcaagg cctccgacca ctggaacgtc agcagacgcc
     2041 acaccaccac cagctacggc ccgaaccaga gggttctccc gaggccgctc tcggtctcga
     2101 gaaacgcggc tttcaagatc cgcacgccgg ccaggggctc ttacagggtc tacggctggt
     2161 ggcccgccga ccgtgactac aacgcccgca cggtgttcag gatcaagacc acgggcggct
     2221 ggacgaggcg aaccgtcaac cagcagatca acggtggaag gtggatctac ctgggcacgt
     2281 acaacctggc cgaaaacgac tcctaccggg tggaggtctc gagcaagagc ccgggcaggg
     2341 gaagggtcgt cgctgacgcc gtgaaggtaa cgaggagcta aggacccgga ggggaaagat
     2401 gagtatcgag cgaggacgcc tgctggtgga ccgcagagat ttcttgagga tcggcggcac
     2461 gggcctggcc ggtgctgtgc tgctgggtgg cgcgaccacg gcggccttcg ccaggacggg
     2521 tgccttgagc aaggagttcg gggcggcagc cagagagtat cgggtaccgg aggagttgct
     2581 cctggcgatg ggctacgtaa acaccctctg ggagatgccc cctccctcgg caagcgacta
     2641 cgagccgggg gatctcagcg gtcgtggcgc ttatgggatc atgcagctcg tgcagaaccc
     2701 gtgggcggat acgctggcca gggccgccgc gcttaccggt ctctcccagg cctcgctcaa
     2761 ggcttccaga ccggccaata ttcgaggcgg ggctgcggtc ctcgcacaga tgcaaggaac
     2821 ggagagcccc ggggaccttg gcgcctggca acaggccgtg gccgaatacg ggggcggcgc
     2881 gctctacgcc gaacaggtct acgaaacgct caaggatggg gcttcggcga ccatctcgac
     2941 cggggaacgg ctggagatag caccgcacga ggacgtcgag gctcccgcca cttacacggc
     3001 cgcatccacc ccggactaca agagggccag gtggcgtcct gcctacgggg gcaactacac
     3061 gaactccaat cgcgaagcct cctacaacat aggcaggatc gtgatccaca ccgtggaggg
     3121 gtcctactcc agcgccataa gctggtttca gaaccctcgc gcgaacgtct ccgcccacta
     3181 cgtcgtcagc aggagaggcg atgtcgccca gtgcgtacgc gacgccgacg tcgcctggca
     3241 cgccggaaac tggaactgga acacgcacag cataggaatc gagcacgaag gctactccgg
     3301 taaccgctcg acgtggacca acgccatgta ccacgcgtcg gcgc
//
LOCUS       DBNGVD010000025         7700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_185700, whole genome shotgun sequence.
ACCESSION   DBNGVD010000025 DBNGVD010000000
VERSION     DBNGVD010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_185700"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..895)
                     /locus_tag="AC16CM_00655"
     CDS             complement(80..895)
                     /locus_tag="AC16CM_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3138365.1"
                     /translation="MRPVCFISDYGLSGELVGICRGVMVGIAPGVTVIDVTHSIPKFD
                     VVSGAETLRHATGYMPEDAVYLAVIDPDDKPVRRELAAETGSGALLIGPDNGLLMPAA
                     RALGGVNRAVSLTNREYHLHPVSHSFHGRDVFSPVAAHLAAGVELGELGEDVDPASLS
                     PIDFPEARKDGDGLVAEIIDIDSFGNARLSATSQDLDADYGTQLEVSVRDETMEATYV
                     QTFGSSYTGDLVLLTDSHWRLSLAVNRGNASRALLLSTGDGVSIKRARDGSSP"
     gene            1042..1524
                     /locus_tag="AC16CM_00660"
     CDS             1042..1524
                     /locus_tag="AC16CM_00660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3138366.1"
                     /translation="MSPLAAIIADTHMPRRARTLPGGMIPHLERADLILHAGDLMDPT
                     LLGELSTYAPTRAVRGNLDLPEDGLPETLELEFGGAKVAMIHDSGPRRGRRARMGRRF
                     PEARVVVFGHSHIPWLEDENGLLLLNPGSPTDKRRQPDHTFALLRAEGGEVAAEILVL
                     "
     gene            complement(1632..3116)
                     /locus_tag="AC16CM_00665"
     CDS             complement(1632..3116)
                     /locus_tag="AC16CM_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3138367.1"
                     /translation="MESTGLEKKYSAGKGRRRVTLWVAVAFLTLAQGIAVLLFLRHGW
                     QALGFPYPLNYGEGPLLDQAVRLARFENIYRTNLSTPPYVVANYPPLFMAAQVPFVWL
                     FGPAMWYGRLISLLSVAAVSLFVGLTLHALTRDRLASLAGALVFPAMPYVVRWSSLSR
                     VDSLGLALSWAGLFVVARWPQKRWSVFVSALLLVAAVYTRQTYILAAPLAAFVWLVAQ
                     GQRRRALQTAAIAGGACLLLFAALNISTGEGFFLNTVTANLNDFRWERVSLNALGALL
                     ACPLLLLASLAFLLLTPRKGNNAWWLVAPYLIASIPTAVLVGKVGSDVNYLLELSAAL
                     GLATGAFMAWQRRRPRLRIVLIAFLAIQMLALSQSARVATGLQNYVMDQRAEVAQLST
                     MVANTEGPVLTDDYMGLLPERGKRIFFQPFEMTQLARDGDWNQRPFVEDIANERFPLI
                     MIWSPPFAREIKQDRWTPRMLEEMGNHYERTGRVADMVIYRPKE"
     gene            3247..4860
                     /locus_tag="AC16CM_00670"
     CDS             3247..4860
                     /locus_tag="AC16CM_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3138368.1"
                     /translation="MQRAQDLAEGVAREDSEQFRIDARDFLSARERGGTTGTRIDQQT
                     AALSDAAEIVNRSFPTLEPSGRFINAISPVVIPNRQSLFWRLMPIGMMLVFFLLGAMA
                     SLGIIGLAGGGVVLALLGPHLWLLLILLAFFSWWRQSVVAVPDGSMAVITRFGKLEEV
                     VGPGRKLLFNPWKQVSYIVNTTREYPYNAPIRQAPTASRVNASVDLFLQFRIEDPAEF
                     IFTVGGVKGFSDKLENAISEVTRALIYEQRAEDIYDLVGESTQKFLDSLNEQFLPAVR
                     FTNANITHAEPSSQEYRMDLAAPEMVRVAKEAYTYEYELRLRKEQDEGELRGELASLR
                     ETLSAISAEIATYQAQIDTARERETNRANASARERMVEAESTANANAALLEAQALDIR
                     AVSSAAAPEILEYRFQQDVLERLAAVADRLPQVVQIGSHDGDDAVDFLQISRRMIGTG
                     SEPLFTPEDMQAIRDRMEEIQARIRERSKELDSLSNGHEAPPEDEPLQQPAAEEENAD
                     RIEEIRQSVTDQAVRERVERIGSDGEEGR"
     gene            4926..6191
                     /locus_tag="AC16CM_00675"
     CDS             4926..6191
                     /locus_tag="AC16CM_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3138369.1"
                     /translation="MQEFSRIKEVVSGWSGVRQYLRGGDAGQIVPVVIPKDRRRYGWL
                     FLLGIGAYLLGFALFSGSAVLAVLTVLLAVLFFGLALLWLWRGAIVEIEQGTTGVRSR
                     YGRIEGTLPPGRHYLWRPWDKVEFVVDTATEIPYLAPVAVCPTRENVPLKAIEFFLKF
                     RIVEPLAFVRVIGASNFDLVLSSAVQDAIRQRSRQVSTEKAYDLRGSDVGDMQNYLNR
                     QLAQYGVRILGANIPDVQLPDQYQQHLATRERVAKELAAYEREWELTRKQRIDTLLME
                     IERSKKDRAARLIEVKEARNRARQDVARMLEEQETEAQRVRLEIETRGRAALTEAENE
                     ATALRHLGQAHRDNRAVVRYELARRRVEIGEKLMKRAPRPFLIRGEGGEQSALSTLLL
                     ARLLPQLTQNGNSVQDASEALRDLADGEG"
     gene            complement(6242..7387)
                     /locus_tag="AC16CM_00680"
     CDS             complement(6242..7387)
                     /locus_tag="AC16CM_00680"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR3138370.1"
                     /translation="MSIRTLVNPKAGESFVGPTPDRSPLEFHRRLPGYEETPLVDAPR
                     LAEALGVGKVLVKDESSRLGLPAFKVLGASWAVYRALVERLGDRGFGGWQTIEDLRQR
                     LEPLRPLSLVAATDGNHGRALARVARLLGLRARIFVPGDMAAARREAIAGEGAEVVVV
                     DGGYDEAVERSSEAEGLLISDTSWPGYERVPTWVIEGYSTMLWEIEDQLAGRDEPGPD
                     LVVAQVGVGAFAAAVARHFRRPEVPHSPKLVTVEPARADCLFRSVSAGRLVTVPGPHD
                     SIMAGLNCGRPSLVAWPILSSTIDVLVTMDDDPAREAMRLAARSGIVSGETGAAGLGC
                     LLELLHSKEHEGTRRLLGANGNTRVLLFNCEGATDPEAYRRIVPDQS"
     gene            complement(7513..>7700)
                     /locus_tag="AC16CM_00685"
     CDS             complement(7513..>7700)
                     /locus_tag="AC16CM_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HIR3138371.1"
                     /translation="NYKVRILDEHRATAATTRVLIDSTDGKRMWGAVGVSENIIEASW
                     QALVDGLEYGVNGPKKT"
BASE COUNT         1400 a         2490 c         2554 g         1256 t
ORIGIN      
        1 tgggcaacag gcatcaggta attttcctga tgcctgttgc ccttcaccgc tgaacggacc
       61 atttttctcg agagctgccc tagggtgatg acccgtccct ggcccgcttt atgctgacgc
      121 cgtctccggt agagagcagg agggctctcg aggcgtttcc cctgtttacg gcgaggctca
      181 ggcgccagtg ggagtccgta agcaacacga ggtcaccggt gtaggatgac ccgaaggtct
      241 gcacgtaggt cgcctccatg gtctcatcgc ggaccgagac ctccagctgg gtaccgtagt
      301 ccgcgtcgag atcctgggag gtggcggaga gcctggcgtt tccgaagctg tcgatgtcga
      361 tgatctcggc cacgagaccg tcgccatcct tacgggcctc gggaaaatca atgggcgata
      421 gcgaggccgg gtcaacgtct tcccccagtt caccgagctc gacgccggcg gccaggtggg
      481 cggccacggg cgagaagacg tcccttccgt ggaagctgtg tgagacgggg tgcagatggt
      541 actcccggtt cgtcagcgac acggcccggt tgaccccccc gagagccctg gcagcgggca
      601 tgagcaggcc gttgtccggt ccgataagga gggccccgct acccgtctcg gcggcgagct
      661 cccgccttac cggcttgtcg tccgggtcta tgaccgccag ataaacggcg tcctcgggca
      721 tgtacccggt ggcgtgccgc agcgtctcgg ctccgctgac cacgtcgaac ttggggatgc
      781 tgtgggtcac gtcgatgacg gtgacaccag gcgcgatacc aaccatcacg cccctgcaga
      841 tcccaaccag ctcaccggag aggccgtagt cggatataaa gcaaactggt cgcatcgaca
      901 cagtatagac ggtaagggta ccttcccgcc tcggacgtcg gggccccgga ttcgtcatga
      961 tttacgccac gggggaacct cgatcgtgta gggtcgaact cgaacacctg ccagaccacc
     1021 aacaccagac aggaggaccg tatgagcccg ctcgccgcca tcatcgccga tacgcacatg
     1081 ccgcgacgcg ccaggacgtt accgggaggt atgatccccc atctggagcg cgcagacctc
     1141 atcttgcacg ccggcgacct gatggatccc acgcttctgg gggagctgtc gacctacgct
     1201 cccaccaggg ccgtgcgggg aaacctcgac ctccccgagg acgggctgcc cgagacgctg
     1261 gagctcgagt tcggcggcgc gaaggtggcc atgatccacg actccggccc gaggcggggc
     1321 cgccgggcca ggatggggcg gcgttttccc gaagccaggg tggtcgtctt cgggcactcg
     1381 cacatcccct ggctggagga cgagaacggg ctattactcc tcaaccccgg cagccccacg
     1441 gacaaacgga ggcaaccaga ccacacgttt gccctgctgc gggccgaggg cggcgaggtc
     1501 gcggccgaaa ttctggttct gtgagcggcg cgtagggaca tcatttgcgg cgtcccgaac
     1561 agcgtatggt cgttgggcag ttcgcgaacc gcccctacac cgtgcggagt tgacccgcca
     1621 cgtgcctgta cctattcttt cggacggtat atgaccatgt cggcgacgcg gcccgtcctc
     1681 tcgtagtggt tgcccatctc ctctagcata cgtggggtcc acctgtcctg ttttatctcc
     1741 ctggcgaagg ggggactcca gatcatgatg agggggaacc tctcgttggc tatgtcctcg
     1801 acgaaggggc gctggttcca gtcgccgtcg cgggcgagct gggtcatctc gaagggttgg
     1861 aagaagatcc tcttccccct ctccggcaag agccccatgt agtcatcggt gagcaccgga
     1921 ccttccgtgt tcgcgaccat ggtagatagc tgcgcgacct cggccctctg gtccatcacg
     1981 tagttctgga ggcccgtggc cacgcgtgcg gactgggaga gcgcgagcat ctggatcgcc
     2041 agaaaagcga tgaggactat gcgcagccgc gggcgtcggc gttgccaggc catgaacgcg
     2101 cccgtggcga ggccgagggc cgcggagagc tccagcaggt agttaacgtc cgagcccacc
     2161 ttgcccacca gcacagccgt ggggatactg gcgatgaggt agggtgcgac gagccaccac
     2221 gcgttgttgc ccttgcgcgg cgtgagcagg aggaacgcga gcgaggcgag caggagcaag
     2281 gggcaggcca ggagggcgcc gagggcgttg aggctgaccc gctcccagcg gaagtcgttg
     2341 aggttggcgg tcacggtgtt gaggaagaaa ccctccccgg tggagatgtt cagggcggcg
     2401 aacaggagca ggcaggcgcc gccggcgatc gccgccgtct gtaatgcccg gcggcgctgg
     2461 ccctgggcga cgagccacac gaaggcggcg agcggcgcgg ccaggatgta ggtctggcgc
     2521 gtgtacacgg cggccacgag tagcagggcc gagacgaaga cgctccagcg cttctggggc
     2581 cagcgggcca cgacgaagag ccccgcccag gagagggcca gcccaagcga gtccacccgc
     2641 gagagggatg accagcggac cacgtagggc atggcgggga agacgagggc gcccgccagg
     2701 gaggcgagtc gatccctggt cagggcgtgc agcgtcaggc cgacgaaaag tgacacggcg
     2761 gcgacgctga ggagtgagat caggcgtccg taccacatcg ccgggccgaa gagccacacg
     2821 aacggcacct gtgcggccat aaagagcggg gggtagttgg cgaccacgta gggaggcgtg
     2881 ctgaggttgg tcctgtaaat attctcgaac cgcgcgagcc tgacggcctg gtcgagaagg
     2941 ggcccctcac cgtagttgag cgggtagggg aaacctagcg cctgccaccc gtggcgcaag
     3001 aagagcagca cggcaatacc ctgcgcaagc gtcaggaagg cgaccgcgac ccagagcgtg
     3061 accctgcgcc ttcccttgcc cgcactatat tttttctcca gaccggtact ctccaccagg
     3121 ccctctcgcg ctatctgact caggtttcgt atcctacctc ttttggctcc ggcgccgcgc
     3181 gaacgagatt gggtcgcacg gtgtagtatg aaggcagtat ctcctagaat tctggaggat
     3241 ttcttggtgc agagggcgca ggatctcgcg gaaggcgtag ctcgggaaga ctccgagcag
     3301 ttcaggatcg atgccaggga ctttctctca gcgcgggagc gcgggggaac cacgggcacc
     3361 cgcatagacc agcagacggc ggccctctcc gacgcggccg agattgtcaa ccgcagcttc
     3421 cccaccctgg agccttcggg ccggttcata aacgccatct cgcctgtcgt catcccgaac
     3481 cggcagtcgc tgttctggcg ccttatgccc atcgggatga tgctcgtgtt cttcttgctc
     3541 ggcgcgatgg cgtcgctcgg catcataggc ctcgcgggcg gcggggtggt gctggcgctc
     3601 ctgggacccc acctgtggct gctcctgatc ctcctggcct tcttctcctg gtggcgccag
     3661 agcgtggtgg cggtcccgga cggcagcatg gccgtgatca cccgcttcgg caagctggag
     3721 gaggtcgtag ggcccgggcg caagctgctc ttcaacccgt ggaagcaggt cagctacatc
     3781 gtcaacacca ccagggagta cccctacaac gcgcccatca ggcaggcccc gacggccagc
     3841 agggtgaacg cgagcgtcga cctcttcctg cagttcagga tagaggaccc ggccgagttt
     3901 atctttacgg tcggcggggt caagggtttc tcggacaagc ttgagaacgc catcagcgag
     3961 gttacgcgcg ccctgatcta cgagcagcgc gccgaggata tctacgacct cgtgggggag
     4021 agcacccaga agttccttga ttccctgaac gagcagttcc tgccagcggt gcgcttcacc
     4081 aacgcgaaca ttacccacgc cgagccctcc agccaggagt accgcatgga cctcgccgcc
     4141 cccgagatgg tcagggtggc caaggaggcc tacacctacg agtacgagct caggctgcgc
     4201 aaggagcagg acgaaggcga gctgaggggt gagctcgcct cgttgcgcga gaccctgtcc
     4261 gcgatcagcg ccgagatagc gacctaccag gcccagatcg acaccgcccg cgaacgtgag
     4321 acgaaccgcg ccaacgccag cgcccgcgag aggatggtcg aggccgagag caccgccaac
     4381 gccaacgcgg ccctcctgga ggcgcaggcg ctggacattc gggctgtaag cagcgctgcg
     4441 gcgccggaga tcctggagta ccgcttccaa caggacgtcc tggagaggct ggccgccgtc
     4501 gcggacaggc tgccccaggt ggtccagatc gggagccacg acggcgacga cgcagtcgac
     4561 ttcctacaga tctcgcgccg tatgatcggg accggcagcg agccgctctt tacgcccgag
     4621 gacatgcagg ccataaggga caggatggag gagatccagg cccgcatccg ggaacgctcc
     4681 aaggagctcg acagcctctc caacggccac gaggcacctc ccgaagatga accgctacag
     4741 cagccggctg ccgaagagga gaacgcggac aggatcgagg agatccgtca gtccgtcacc
     4801 gaccaggccg tgcgcgaacg cgtcgagcgt ataggctccg atggggagga ggggcgatga
     4861 ccgagacggt gcagtccgta caggcgcaga tgcaaccgca ggtccaggcg cagacaggcg
     4921 ccaggatgca ggagttctcc aggatcaaag aggtcgtctc gggctggtca ggggtcaggc
     4981 agtacctgcg gggcggcgac gccggccaga tagttcccgt ggtgatcccg aaggaccgca
     5041 ggcgctacgg ttggctcttc ctcctcggta tcggggctta cctgctgggc ttcgcgctct
     5101 tctcggggag cgcggtgctc gccgtgctga ccgttctgct cgccgtcctg ttcttcgggc
     5161 ttgccctgct gtggctgtgg cggggcgcta tcgtagagat cgaacagggc acgaccggcg
     5221 tgcgctcgcg ttacgggagg atagaaggca ccctcccgcc gggccgccac tacctctggc
     5281 ggccgtggga caaggtagag ttcgtcgtcg atacggcgac cgagatccct tacctcgccc
     5341 ccgtcgccgt ctgcccgacg cgcgagaacg tgcccctcaa ggcgatcgag ttcttcctga
     5401 agttcaggat agtggagccc ctggccttcg tgcgcgtgat cggggcctca aacttcgacc
     5461 tggtcctctc cagcgccgta caggacgcca tccgccagcg cagccggcag gtgagcacgg
     5521 agaaggccta cgacctgcgc ggcagcgacg tgggcgacat gcagaactac ctgaaccgcc
     5581 agctcgccca gtacggggtt cgcatcctcg gggccaacat ccccgacgtg cagctccccg
     5641 accagtacca gcagcacctc gccacccgcg agcgcgtggc caaggagctc gccgcctacg
     5701 agcgcgagtg ggagctgacc cgcaaacagc gcatcgatac cctgctcatg gagatagagc
     5761 gctccaagaa agaccgcgcc gcccgcctca tcgaggtaaa ggaagcccgc aaccgcgccc
     5821 gccaggacgt ggcgcgcatg ctagaggagc aggagaccga ggcccagcgc gtgcgcctgg
     5881 agatagagac gcgcggcagg gccgccctga ccgaggccga gaacgaggcc acggccctcc
     5941 gccacctcgg ccaggcccac cgcgacaacc gcgccgtggt ccgctacgag ctggcgcgcc
     6001 gcagggtcga gatcggtgag aagctcatga aacgcgcccc acgacccttc ctgatccgtg
     6061 gcgaaggcgg agagcaatcg gccttgtcca cccttctcct cgccaggctc ctcccacaac
     6121 tgacccagaa cgggaacagc gtgcaggacg ccagcgaggc cctgcgggac ctggcagacg
     6181 gcgagggctg aaaaaaggct acaggtatca ggcttcagtt agagctgata gctgacggct
     6241 atcagctctg atccggcact atcctgcggt atgcctcggg gtcggtggcg ccttcgcagt
     6301 tgaagaggag gacgcgggtg tttccgtttg cgccaagcag ccggcgtgtc ccctcgtgtt
     6361 ctttggagtg caggagttcg agcaaacagc cgaggccagc agccccggtc tcgccggaga
     6421 ctatgccaga cctggccgcc aggcgcatcg cctcgcgtgc cgggtcgtcg tccatggtca
     6481 ccagtacgtc gatggtactg gacaggatcg gccacgccac gagggatggt ctgccgcagt
     6541 tgaggccggc cattatggag tcgtgcgggc caggcacggt gacgagccgt cccgccgaga
     6601 cggatcgaaa gaggcaatcg gccctcgctg gctccacggt gacgagcttc ggactgtgtg
     6661 gtacttcggg acggcggaag tgtctggcta cggccgcggc gaaggcgccg acgccaacct
     6721 gcgcgaccac caggtctggc cctggttcgt ctcttcccgc gagctggtct tcgatctccc
     6781 agagcatcgt ggagtagccc tcgatcaccc aggttggaac acgctcgtag ccgggccagg
     6841 aagtgtcgga gatcaggagc ccctccgcct cggacgatct ctcgacggct tcgtcgtagc
     6901 cgccatcgac cacgactacc tcggccccct ccccggcgat tgcctcgcgc ctcgccgcgg
     6961 ccatgtcccc tggaacgaag atcctcgccc gcaaccccag cagccgcgcc acccgcgcca
     7021 gggcacgccc atggttgccg tcggtggcgg ccacgagcga gagcggccgc aagggttcca
     7081 gtcgttgcct caggtcttcg atggtctgcc acccaccgaa ccctctgtcc ccaaggcgtt
     7141 ccactagcgc cctgtatacg gcccacgagg cgccgaggac cttgaaggcg ggcagcccga
     7201 gccggctcga ctcatctttc acaagtacct tgccaacgcc gagagcctcg gccagtcgcg
     7261 gagcatcgac gagcggtgtc tcctcgtaac ctggcagccg gcggtggaat tcaagaggcg
     7321 aacggtcggg cgtcggcccg acgaagctct ctccggcttt tgggttgacg agggtcctga
     7381 tgctcatggg aaatcgattc tagatcgtct ctccgggaag agcgaaccca gggtgatcga
     7441 tggccacggg catcgggcca tctttcctga agcctgaaac ctgtagcctg atgtcttgtc
     7501 cccgattcgt catcacgtct tcttcggccc gttcacgccg tattcgaggc cgtctaccag
     7561 agcttgccag gaagcttcga tgatattttc ggagacgcct acggcgcccc acattcgctt
     7621 gccgtctgtc gagtctatga ggacgcgggt cgtggcggcg gtggcgcggt gctcgtccag
     7681 gatacggacc ttgtagttgg
//
LOCUS       DBNGVD010000026         2860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4923044, whole genome shotgun sequence.
ACCESSION   DBNGVD010000026 DBNGVD010000000
VERSION     DBNGVD010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4923044"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16CM_00690"
     CDS             <1..275
                     /locus_tag="AC16CM_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3138372.1"
                     /translation="GTDAVVIIGELGGTMAEEVAGAIEVGSFTKPLVASLGGRTAPEG
                     TRMGHAGAIVTAGQGTVEGKISVLERAGALVADRPSNVGRLLREVV"
     gene            336..1613
                     /locus_tag="AC16CM_00695"
     CDS             336..1613
                     /locus_tag="AC16CM_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3138373.1"
                     /translation="MTIEVFSLLVLLVMFVVASVLPINLGIMGFVAAFGVGALLGGLT
                     ADDIFAVFPGDLFILLAGVTFLFAIAQNNGTVDLLMNWGVRLVRGNVGLVPWIMFTLT
                     TLVTGVGALSSAGVAIVAPVALRSAAQYRISPLLMGIMVVAGATAGSYSPLSPFGIIT
                     NGVLASRDLPQYPGLLFINSLAFNAVVAALVFVAFGGLRLLRRRISLEEIMSDNGSEK
                     SDEGTLTPYRAATLAGIVILAISAFAFGSSDRFDIGFAAFIIGLVLALMAPREQGAAL
                     GRMPWPAILLICGILTYVGVLDQIGTIEYMQNLIAGVGNAVVAALAASYVGGIISAFA
                     STAAVLGASIPLAEPILRDSSLSAIGVITAIAISSAIVDISPLSTNGALLMANAQGID
                     ERKFFRQLLAWAVGVVIFAPLLAWLVFVVIGIP"
     gene            1617..>2860
                     /locus_tag="AC16CM_00700"
     CDS             1617..>2860
                     /locus_tag="AC16CM_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277762492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR3138374.1"
                     /translation="MRLGQREKELLGGAHGETVAEALRGQIEVGDFFGAERFVPVGNV
                     HMMGDIEVMGEGGLEFLRRVAESGARCAVNTTTNARCFDFGYAGRLGQDPENVARERV
                     LISYLREIGVMTTDTCINYQAVYQPHLGEHVAWGDTGTVIYANSVLGARSNFEGGPAA
                     LAASLTGVTPAYGFHLDENRRGNILVQLEATLESLSDWGAVGRIVGEGHQDYTSVPVF
                     TGIDRSPTADELKHLGASLASHGSMGMYHIVGVTPEARTVDDAFGSNAPQATVTVTDE
                     DLENLYATYETGDGDANLVVFSAPQLSLYELKTLSELLQGKRVSEGTSLIVTTNGAYK
                     AAGEALGYLRDIEEAGGLVLEGVCFYILDGVARMREENGWDNLVTNSAKLANIIKAHR
                     YNPVLRPTEECVRIAVTGRIGGS"
BASE COUNT          465 a          930 c          961 g          504 t
ORIGIN      
        1 cggggacgga cgccgtggtc atcatcgggg agctcggtgg cacgatggcg gaggaggtgg
       61 ccggggccat agaggtgggg agctttacca agccgctggt cgcgtcgctc ggggggcgca
      121 ccgctcccga gggcaccagg atgggtcacg ccggcgccat agtcaccgcc ggacagggaa
      181 cggtggaggg caagatctcg gttctcgaac gggccggcgc cctcgtggcc gaccgtccga
      241 gcaacgtcgg gaggctgctc cgggaagtcg tctagcgggt cgtactcgag aacgggaggg
      301 gacggagttt tggattacgc gctgggggtc tgttcatgac catcgaggtc ttttcgctgc
      361 ttgtgctgct cgtcatgttc gtggttgcct cagtgttgcc gatcaacctc ggcatcatgg
      421 ggttcgtcgc ggccttcggg gtgggcgcgc tgcttggcgg cttgaccgca gacgacatat
      481 tcgcggtctt ccccggcgac ctgttcatcc tgctggcagg ggtcaccttc cttttcgcca
      541 tcgcgcagaa caacgggacc gtggacctgc ttatgaactg gggtgtcagg ctcgtcaggg
      601 gcaacgtcgg gctcgtgccc tggatcatgt ttaccctcac caccctggtt accggcgtcg
      661 gcgcgttgag ctcggccggc gtggccatcg tggcgccggt cgcgctccgg tccgccgccc
      721 agtacaggat cagccccctg ctcatgggca ttatggtcgt agcgggggcc acggcgggct
      781 cctactcgcc gctcagcccc ttcggcatca tcacgaacgg cgtgctggcg tcgagggacc
      841 ttccgcagta ccccgggcta ctcttcatca actcgctggc cttcaacgcc gtggtcgcgg
      901 ccctggtgtt cgtggctttc ggcggcctga ggctgctcag gagaaggatc tcgctagaag
      961 aaattatgtc cgataacggc tcagaaaagt ccgacgaggg taccctgacg ccgtacaggg
     1021 ccgccacgct cgcgggcata gtgatccttg ccatctcggc gttcgccttc gggtcgagcg
     1081 acaggttcga catcggcttc gcggcgttca tcatagggct ggtgctcgcg ctcatggccc
     1141 cgcgggagca gggcgcggcc ctgggcagga tgccgtggcc ggcgatactg ctgatctgcg
     1201 gcatcctcac ctacgttggc gtgctcgacc agatcgggac catagagtac atgcagaacc
     1261 tgatcgccgg cgtcggaaac gccgtcgtcg cggcgcttgc ggcctcctac gtgggcggca
     1321 taatctccgc cttcgcctct accgcggccg tgctaggtgc ttccataccg ctagcggaac
     1381 ccatcctgcg agactcgagc ctctccgcca tcggggtgat cacggccatc gccatctcct
     1441 cggctatcgt ggacataagc ccgctgtcga ccaacggggc gctgttgatg gccaacgccc
     1501 agggcatcga cgaacgcaag ttcttccggc aactgctggc gtgggcggtc ggggtcgtga
     1561 tcttcgcccc cctcctcgcc tggctggtgt tcgtcgtgat cggcatcccc taaccggtgc
     1621 gcctgggcca gagagagaag gagctgctgg gcggagcgca cggcgagacg gtcgcagagg
     1681 cgctccgcgg ccagatcgag gtgggcgact tcttcggggc cgaacgcttc gttcccgtgg
     1741 gcaacgtcca catgatgggc gatatcgagg tcatgggcga gggcgggctg gagttcctgc
     1801 gccgcgtggc cgagtcgggg gcgcggtgcg ccgtgaacac gacgaccaac gcgaggtgct
     1861 tcgacttcgg atacgccggg cgcctgggcc aggacccgga gaacgtggcc agggagcggg
     1921 tcctgatctc ctacctccgc gagatcgggg tgatgaccac ggacacgtgc atcaactacc
     1981 aggccgtcta ccagccccat cttggggagc acgtggcctg gggtgatacg gggaccgtga
     2041 tctacgccaa ctccgtcctc ggcgcgcgct cgaacttcga gggcggcccg gccgccctgg
     2101 ccgcctccct gacgggcgta acgcccgcct acggctttca cctcgacgag aaccgccgcg
     2161 gcaacatcct cgtgcaactc gaggccaccc tcgaaagcct ctccgactgg ggcgccgtgg
     2221 gccggatagt cggcgaggga caccaggact acacctccgt ccccgtcttt accggcatcg
     2281 accgatcacc aaccgcggac gagctcaagc acctcggcgc ctccctggcc agccacggct
     2341 cgatggggat gtaccacatc gtcggcgtca cacccgaggc gaggacggtg gatgacgcgt
     2401 tcggttcgaa cgcgccacaa gccaccgtca ccgtgaccga cgaagacctc gaaaacctct
     2461 acgccaccta tgagaccggc gacggggacg ccaacctcgt cgtgttctcc gccccccaac
     2521 tctccctcta cgagctcaaa acgctctcgg agcttctcca gggaaagcgg gttagtgagg
     2581 ggacatctct gatagtcacg accaacgggg cctacaaggc ggcgggcgag gccctcggct
     2641 acctgcggga catcgaggag gccggtggcc tggtgctcga aggcgtgtgt ttctacatcc
     2701 tcgacggggt ggccaggatg agggaggaga acggctggga caacctcgtc accaactccg
     2761 ccaagctggc caacatcatc aaggcgcaca ggtacaaccc cgtcttgcgc cccacggagg
     2821 agtgcgtcag aatcgccgtg acgggtagga tcggggggtc
//
LOCUS       DBNGVD010000027         3871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1825980, whole genome shotgun sequence.
ACCESSION   DBNGVD010000027 DBNGVD010000000
VERSION     DBNGVD010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1825980"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16CM_00705"
     CDS             <1..263
                     /locus_tag="AC16CM_00705"
                     /inference="COORDINATES: protein motif:HMM:NF039957.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HIR3138375.1"
                     /translation="RKQLGDRRVLVLETIGVLVFAALALGALLAEGRTAQYLLAAAWL
                     GHGAWDVAHHVADRVVPRWYAEICAVLDVLVAVGIILAVPHV"
     gene            454..828
                     /locus_tag="AC16CM_00710"
     CDS             454..828
                     /locus_tag="AC16CM_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138376.1"
                     /translation="MGRHLLEMIAAMMIGMMLLVAGILAFGEPPGYSNVFVEYGLMGA
                     SMSGAMVAWMRFRGHAWWDGLEMTTAMLVPMFAVVFPFGWDTAGGTSGQSDHALMMLA
                     HVAMIGGMVALMLYRWDRYTHG"
     gene            complement(870..2057)
                     /locus_tag="AC16CM_00715"
     CDS             complement(870..2057)
                     /locus_tag="AC16CM_00715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3138377.1"
                     /translation="MNFDPTEAQRRWRDLARDFAQNEIRPHAAELDREQRFPYGIIAD
                     MASLGLLGLTLPEEYGGSGGDFVAYNLALEEISRADTSVGITMEAHISLGCAPILAYG
                     TPEQKERYLPRCATGENLWAFGLTEEGAGTDAGGTRTTARLEDGYWVVDGSKKWITNS
                     GTDITGGVTVIARTGTRENGKPELTAIIVPQDTPGYTRGPGYEKLGWRAADQHPLYFD
                     DCRVPEENLLGERGRGFSQFLATLDAGRVAVAALSVGLAQACFDEAVARAKEREQFGR
                     AIAGFQAIQFKLSDMAMETELARNLVLKAAWLRDMGRPFGREASMAKVFASEAAKRAA
                     DEAVQIWGGEGFMETSAVARYWRQVKINEIGEGTSEINRQIIARSLLTETPLEAKQQM
                     GTP"
     gene            2233..2496
                     /locus_tag="AC16CM_00720"
     CDS             2233..2496
                     /locus_tag="AC16CM_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3138378.1"
                     /translation="MLGAIIFWIIVGGIAGALAKLIMPGDDPGGFVITILLGIAGAIV
                     GGFLGSFIGIGGGGWIWSILIATIGAIILLAIYRLLAGNRATR"
     gene            complement(2716..3672)
                     /locus_tag="AC16CM_00725"
     CDS             complement(2716..3672)
                     /locus_tag="AC16CM_00725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HIR3138379.1"
                     /translation="MTHNHRLSESSPHDLGRAALVVNTRSRTGERAFFQAIDRLEELG
                     IPLGATYAIRDPARLSETVREVVEDGHRLVILGGGDGSVSSVVDFLAGRDVVLGLLPL
                     GTANDFARTLQMPEDVESACQAIAGGKIVDVDLGLAGDNYYVNVASVGLSVGATRSLS
                     PWLKKSTGPLAYPLAAMRAFLRHEPFSGRLTFPDGDHEPVEYDRLLQVAVGNGRFYGG
                     GMIVAPESGIDDKTLDVYAIELGRSRDLFGVARYLKSGDFINVEGVHHFRTPRVRLEA
                     DPDQQVNIDGEVVTRTPQNFSVARNALDVLVPQESSAAHHDD"
BASE COUNT          642 a         1187 c         1297 g          745 t
ORIGIN      
        1 tccgcaaaca gctgggagac cgcagggtgc tggtgctaga gacgattggg gtactggtct
       61 tcgctgccct cgcgctgggc gcgctgcttg cggagggacg gaccgcccag tacctcctgg
      121 ccgccgcctg gctcgggcac ggcgcgtggg acgtggccca ccacgtcgcc gacagagtag
      181 tacccaggtg gtacgcggag atctgcgccg tactcgacgt gctggtcgcc gtggggatca
      241 tcctcgccgt gccacacgtg tgatgtccag aggtttgtcg aggcactacc caggaaggag
      301 agcatgaagc cagagatcaa caacgtaggg ggcaaccgac ccgaagagac agcagcccca
      361 gcgtgggaag atgcgcaggg gcgaatcgta gaaaccgcct cgtcaggagc gggctggtgg
      421 tcgggacgac ttcgacccgg ttcagcatgg cgtttggggc gacacctcct ggagatgatc
      481 gccgcgatga tgatcgggat gatgctgctc gtcgccggga tcctggcttt tggcgaaccg
      541 cccggctact ccaacgtatt cgtcgagtac ggtctaatgg gcgcgtccat gtcgggggcc
      601 atggtggcgt ggatgcgctt caggggacac gcctggtggg acggcctgga aatgacgact
      661 gccatgctcg tgccgatgtt cgcggtggtc tttccgttcg gatgggatac ggcgggagga
      721 acatcgggcc agtcggacca cgcgctaatg atgctcgctc acgtggcgat gatcggcggt
      781 atggtcgctc tgatgctcta ccgttgggac cgttacacgc acgggtaagg caacggacgg
      841 aaattgtagg ggcgggttcg aacccgcccc tacggggtcc ccatttgttg tttggcttcc
      901 aggggagtct cggtcaggag gctgcgggct atgatctggc ggttgatctc gctcgtccct
      961 tcgcctatct cgttgatctt gacctggcgc cagtagcggg ccacggcgct cgtctccatg
     1021 aagccctctc cgccccagat ctggacggcc tcgtcggcgg cgcgcttggc ggcttcggag
     1081 gcgaagacct tggccatgct cgcctcccgg ccgaaaggac gtcccatgtc cctgagccac
     1141 gctgccttga ggaccaggtt gcgggcgagc tctgtctcca ttgccatgtc ggagagcttg
     1201 aactggatgg cctgaaagcc ggcgatggcg cgcccgaact gctctctctc cttcgcccgg
     1261 gccacggcct catcgaaaca tgcctgggcg aggcctacgg agagggctgc gacggcgacc
     1321 cggccagcgt ctagcgtggc gaggaactgg ctgaagcccc tgccgcgctc gccgagcagg
     1381 ttttcctcgg gcacgcggca gtcgtcaaag tagagcgggt gctggtctgc ggcgcgccac
     1441 ccgagctttt cgtagccggg gccgcgggtg tagcccgggg tgtcctgggg gacgatgatc
     1501 gcggtcaact ccggtttgcc gttctcgcgg gtgcccgttc gggcgataac ggtgaccccc
     1561 cccgtgatgt ccgtgcccga gttcgtgatc cacttcttcg acccgtctac gacccagtac
     1621 ccgtcctcca acctcgccgt cgtccgcgtc ccgcctgcat ccgtcccggc tccctcctcg
     1681 gtaaggccga acgcccatag gttctcgccg gttgcgcacc tggggaggta cctctccttc
     1741 tgttctggcg tgccgtaggc caggatcggc gcgcaaccga gggagatgtg ggcctccatc
     1801 gtgatcccca ccgaggtgtc ggcgcgtgag atctcctcca gagccaggtt gtaggcgacg
     1861 aaatccccac cggaaccgcc gtattcttcg gggagagtaa ggccgagcag gccaagacta
     1921 gccatatccg cgattatgcc gtacgggaat ctctgctcgc ggtccaactc cgcggcgtgg
     1981 ggtcggatct cgttctgcgc gaaatcgcgc gccaggtctc gccagcggcg ctgggcctcg
     2041 gtgggatcga agttcaaagg gggcctcctg cgtactcggt ccaggggcat gatagcagtg
     2101 gtccacgaca gctgagcaaa cgtttctatt gttgcgatta cattaaaccc ttcaaaacgt
     2161 tacgaaacct atgtttccca acctcgtata aggggataga ctcgagagta cacaaccttg
     2221 aaggaggcac aaatgctcgg ggcaataatc ttctggatca tagtaggcgg tatcgcaggc
     2281 gcgctggcca agctgatcat gcccggcgac gatccgggcg gcttcgtcat aaccatcctg
     2341 ctaggaatag ccggagctat cgtaggcggt tttctgggca gcttcatcgg tatcggcggc
     2401 ggcgggtgga tctggtccat cctgatcgcc accatcggtg cgattatcct gctagccatc
     2461 taccgactct tagcggggaa tagggcaacc aggtagcgag caccagtttg tggggccggg
     2521 accgctggtt ccggccctct ttttatgctc ggggttcccg ctctctcctc tgcgggcata
     2581 ccagtaacgc aggatgagcg gtgtggccca tcgtcgcgtt gtttcagggc aggcagcaag
     2641 cctgcttgtt tcgggcatca tgagggctcg ggtctagcag gcgcggcagg atggggcgaa
     2701 ctcgtcgccc gcctgtcagt cgtcgtggtg ggcggccgac gattcctggg gcaccagcac
     2761 gtcgagggcg ttgcgggcaa ccgagaaatt ctgtggcgtc cgcgtgacga cctctccgtc
     2821 aatgttgacc tgttgatcag gatcggcctc caggcggacc cggggggtcc tgaagtggtg
     2881 tacgccttcg acgttgataa agtcgccgct cttgaggtac ctggcgaccc cgaacaggtc
     2941 ccggctccgg cccagctcta tggcgtagac gtcgagcgtt ttgtcgtcga tgccggactc
     3001 gggggcgacg atcatccccc cgccgtagaa gcgcccgttg ccgacggcga cctgaaggag
     3061 ccggtcgtac tcgacgggtt cgtggtcccc atccgggaag gttagcctgc cggagaacgg
     3121 ctcgtgtcgc aggaaggccc tcatggcggc cagggggtag gccagggggc ccgtgctctt
     3181 cttcagccaa ggcgagaggg accgcgttgc ccccacgctc aggcccacgg aggcgacgtt
     3241 cacgtagtaa ttatccccgg ccaggccgag gtccacgtcc acgatcttgc cccccgctat
     3301 ggcctggcag gcgctctcga cgtcttcggg catctgcaag gtgcgggcga agtcgttggc
     3361 cgtgccgagg gggagcaagc ccagaacgac gtcgcggccg gccaggaaat ccaccacgga
     3421 gctaacggag ccgtcgccgc cgccaagaat aacgagccgg tggccgtcct cgacgacctc
     3481 gcgcaccgtc tcggagagcc gcgcagggtc ccttatggcg taggtggcgc cgagcggaat
     3541 gccgagctcc tcgagacggt cgatggcttg aaagaacgcc cgttcgcccg ttcgggagcg
     3601 cgtgttgacc acgagcgccg cgcgccccag gtcatgcggg ctgctctcgg agagcctgtg
     3661 attgtgggtc atgctagccc tgcacctctt cctggagctt ctgcgagtcc gtatacggtg
     3721 aacgcgggtc aaggtttctc gcccccgcac cgaccttccg gttcgtataa catgatccgg
     3781 gccgaaaagc ttgcggaaag gggatcagca taggcgacag gatcggtttt gtgggccttg
     3841 gcatcatggg tctgccgatg gccaggaacc t
//
LOCUS       DBNGVD010000028         5045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2190783, whole genome shotgun sequence.
ACCESSION   DBNGVD010000028 DBNGVD010000000
VERSION     DBNGVD010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2190783"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16CM_00730"
     CDS             <1..573
                     /locus_tag="AC16CM_00730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3138380.1"
                     /translation="DAFLSGPAVDYERFAVLQDQPPAREIETVCFFGDATRERIDFEA
                     LQTIVRAGFRLRLVGGLDRVGRGLLETPGVDYRGEVPHEALPGALAGVDAFVLPYRTN
                     ALTRAVSPAKTYECLAAGRPVVAAPLPALGELRQYLYLARRPEDYVEILRGLWDGDTE
                     EKVRGGIGSARENSWDARFAGLEAAICRAL"
     gene            558..1370
                     /locus_tag="AC16CM_00735"
     CDS             558..1370
                     /locus_tag="AC16CM_00735"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR3138381.1"
                     /translation="MSRPVEGRPSRIDVLGVGVDPLTVEELQAGIKRLVEGGGRGTIL
                     NVNANCLNLLYTDAVLRGFFDRADLVFCDGAGVMLAARLLGKCIPERITYADWAWRLA
                     AFAEAEGLSLFLLGAAPGVAERAARRLRRRHPGLRISGVGHGYFDHRPGSTENEAVLR
                     KVNAADPDILILGLGMPLQERWVMENRDAIGASVVLTGGAVFDHVSGGLRRGPRLLTD
                     NGFEWLARLLVEPRRLWRRYLIGNPLFLLRVLGQRFGNESHKRSAISGQPKS"
     gene            1427..2614
                     /locus_tag="AC16CM_00740"
     CDS             1427..2614
                     /locus_tag="AC16CM_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HIR3138382.1"
                     /translation="MRGIYACRISLVHVVASALVGVCLLLCTSWSAEAAGGSYIDFGR
                     YDSYRALAAGTARGVTFQDGSARLAYGRTTGTLTSPVYPSAHKDTFIPSWNALTPQKT
                     YLRVEMRVRSSGTWTRWWQMGVWAKDTGTVKRHSVNGQRAGSWRVATDTLQSTGSVFA
                     DAYQYRLTLVSREAGRTPRVGAVYVTASNSNRHGESLVGSHRTLWGEGLDVPPRSQMV
                     YENGGEVWCSPTSLSMVMAYWSKKTGKKSLDQTVPRVARGTYDYAYRGNGNWPFNTAY
                     AASYGLESSINRFSSLGQVELWTAKKVPVIASISWGRGDLTGAPLPASSGHLLVIRGF
                     TRSGDVIVNDPAANSNAGVRRVYRRNEVRKVWLENGSGGVAYIIHPEGWQTPGRTQAH
                     GSW"
     gene            complement(2865..3611)
                     /locus_tag="AC16CM_00745"
     CDS             complement(2865..3611)
                     /locus_tag="AC16CM_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138383.1"
                     /translation="MQNEKRSSPYSEERPAVFRFEKWLQTHAGKHPATFYGLYRLARK
                     DQTRMVTPETQLVIEGFPRSANSFARVAFNRAQKGKVRIATGLHVPAQVIRASRWRIP
                     SLVLIRKPKDAVLSFAIRDPISVEQALKYYLSFYETVEHYSDSYVLGTFEQVTGDFGQ
                     IIERLNDRFATSFSHFRHDGRNVELVLARVERNSKRKFGEANWENKASRPVAAREETK
                     RGLERSLDDPETARLLSRAEALYGRMTAGV"
     gene            3691..>5045
                     /locus_tag="AC16CM_00750"
     CDS             3691..>5045
                     /locus_tag="AC16CM_00750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3138384.1"
                     /translation="MLRDISNFKLLRRLASVLALLLADVAALLLGLGVSAWALDGRSD
                     PAIGLAPLLVAAWVVLFTAFRLYDRAPVRRSPGGLVGAAFCWAGLVILGGAIYPETGL
                     GPAETMFVASLALLCAGSLRYLYERVLERIYRRGFGRTPVLVIGPQEERARLRRMMDR
                     AAGAYVLAGELDMGEGGVDLASLREKLDSTGADGVVLMGAERLPDDHLLDLVHSVRLR
                     GIPLRVVPGAFALMRGRTILSDGMGLPLLEVHYPGLDNTQRTLKRVLDVAGSLGGLLI
                     LSPLLLAVAVAVRLDSPGPVLFRQKRVGADEKVFICYMFRSMYRDAELRQAALEDLNE
                     AEGPVFKIRDDPRITRVGRFLRRWSIDELPQLINVLKGEMSLVGPRPLPVRDFLKMEE
                     AHKRRLGAVPGMTGYWQTSGRSDLSFEEMVRLDLYYIENWSLSFDLKIILKTLGAVLN
                     R"
BASE COUNT          824 a         1652 c         1650 g          919 t
ORIGIN      
        1 gacgcgtttc tcagcggccc cgcggtggac tacgagcggt tcgccgtcct gcaagaccag
       61 cctcctgcgc gcgagatcga gaccgtctgc ttcttcggcg acgctaccag ggagcgcata
      121 gacttcgaag cgctacagac catcgtccgt gcggggttcc gcctgcgcct cgtgggcggc
      181 ctcgaccgcg tgggaagggg gctgctcgag acgcctggtg tggattacag gggagaggtc
      241 ccccacgagg cgctgccagg ggcgctcgcc ggcgtggacg ccttcgtgct tccctaccgg
      301 accaacgccc tcacccgcgc cgtctcaccg gccaaaacct acgagtgcct cgctgcgggc
      361 aggccggtcg tggcggcgcc cctccccgcg cttggggaac tccgccagta cctctatctt
      421 gcaaggcgcc cggaggacta cgtggagatc ttgcggggcc tgtgggatgg ggatacggag
      481 gagaaggtgc gtggcggcat cgggagcgcc cgcgagaact cctgggatgc ccgcttcgcc
      541 gggctggagg cagcgatatg tcgcgccctg tagaggggcg tccatcccgg atagacgtgc
      601 tcggggttgg cgtcgacccg ttgaccgttg aagagctgca agccgggata aagcggctgg
      661 tcgagggcgg ggggcggggc acgatcctaa acgtgaacgc caactgcctc aacctgctct
      721 acacggacgc cgtgctgcgc gggttcttcg accgagcaga tctcgtgttc tgtgacgggg
      781 ccggggtgat gctcgcggcg aggctgctcg gaaaatgcat ccccgagcgc attacctacg
      841 ctgactgggc gtggcggctc gccgccttcg ccgaggcaga gggcctctcg ctcttcctgc
      901 tgggcgccgc gcccggcgtg gccgagcggg ccgctcgacg gctccggagg cgacacccag
      961 gcctccgcat ctccggggtc gggcacgggt acttcgatca ccgccccggc agcaccgaga
     1021 acgaggcggt cctgcgcaag gtgaacgctg ccgaccccga catcctcatc ctcggccttg
     1081 gaatgcccct acaggaacgc tgggtgatgg agaacaggga tgccataggc gccagcgtcg
     1141 tcctcacggg tggggccgtc ttcgaccatg tctccggcgg gctgcgccga ggcccgcgcc
     1201 tcctgacgga caacggcttc gagtggttgg cgcgcctcct cgtcgagccc cgccgcctgt
     1261 ggcgacggta cctgatcggc aaccccctct tcctgctaag ggtgctcggg caacgcttcg
     1321 gcaatgagag ccataagcgg tcagccatca gcggtcagcc aaaaagctga gatccccagc
     1381 ctctggcaag tcgcgccgga tgggcgacgc ctgtagaatg catctcgtga gggggatcta
     1441 cgcttgccgg atctcgttgg tacacgtcgt ggcgtcggcg ctcgtcggcg tttgcctgct
     1501 cctgtgcacc tcctggagtg ccgaggcggc gggtggatcc tacatagact tcggccggta
     1561 tgactcctac cgggcgctcg cggctggcac ggccaggggc gttacgttcc aggacgggtc
     1621 ggccaggcta gcttacggga gaacgacggg caccctgacc tccccggtct acccttcggc
     1681 gcacaaggac accttcatcc cctcgtggaa cgcgctcaca ccccagaaga cctatcttcg
     1741 ggtggagatg agggtccgtt cgtccggaac ctggacccgc tggtggcaga tgggcgtctg
     1801 ggcgaaggat acgggtacgg tgaagcgcca cagcgtaaac ggacagaggg ccgggagctg
     1861 gcgggtagcg acggacaccc tgcagagcac gggctccgtg ttcgccgatg cctaccagta
     1921 ccgtctcacc ctcgtctcca gggaggccgg taggaccccc cgtgtcgggg cggtctacgt
     1981 aacggcctcc aactccaacc gccacgggga gtccctcgtg gggtcccaca ggaccctgtg
     2041 gggcgagggc ctcgacgtcc cgccgcgttc gcagatggtc tacgagaacg gcggagaggt
     2101 atggtgcagc ccgacctcgc tctccatggt catggcttac tggtcgaaaa agaccggcaa
     2161 gaagagcctg gaccagacgg tgccgagggt ggcgcgcggc acctacgatt acgcctaccg
     2221 cgggaacggc aactggcctt tcaacacggc ctacgccgcc tcctacggcc tcgaatcctc
     2281 catcaaccgc ttctcctcgt taggtcaggt ggaactctgg accgcgaaga aggtacccgt
     2341 gatcgccagc atctcctggg ggcgtggcga cctgacaggc gcgcccctcc cggcgagttc
     2401 gggccacctg ctcgtgatcc ggggtttcac cagatcaggg gacgttatcg tcaacgaccc
     2461 cgctgcaaat agcaacgctg gcgtcaggcg ggtctacagg cgaaacgagg tccgcaaagt
     2521 atggctcgaa aacggctcgg gcggcgtcgc ctacatcata caccccgaag gctggcagac
     2581 gcccgggcgt acccaggccc atgggagctg gtagaaggag gctacaggtt tcaggcatca
     2641 ggtaatcttc ctgaatcctg aaacgtgatg cccgatccgt tcgtagaatc tcgttgcctg
     2701 gtcgcgggct aacatccgat ccttctccgt tattcccgcc tcgccccacc gtcattcccg
     2761 cgaaagcggg aatccacgcc tgctcggaag acacaggtcc gtgcctgcca cgatcaccgt
     2821 tcaacctgat tccgtacgaa acctgaagcc tgaaacctgt agccttatac acccgccgtc
     2881 atgcgcccgt atagggcttc cgccctggag agcaggcgtg ccgtctccgg gtcgtccagg
     2941 ctgcgctcca gcccgcgctt ggtctcttcc ctggcggcca cggggcggga ggctttgttc
     3001 tcccagtttg cctccccgaa cttcctcttc gagtttcgct ccacgcgcgc cagcaccagc
     3061 tccacgttcc gcccgtcgtg gcggaagtgg gagaaggagg tggcgaacct gtcgttcagg
     3121 cgctcgatga tctgtccgaa gtccccggtg acctgctcga acgttccgag cacgtaggag
     3181 tctgagtagt gctccacggt ctcgtagaaa gagaggtagt acttcagggc ctgctcgacg
     3241 gagatggggt cccgtatggc gaaggagagt acggcgtctt tcggctttcg tataagcacc
     3301 agggatggta tccgccagcg cgaggcccgg atcacctgag ccgggacgtg caggcctgtg
     3361 gcgattcgca ccttcccctt ctgcgccctg ttgaaggcca ccctggcgaa cgagttggcc
     3421 gacctcggaa atccctcgat gaccagttgc gtctcggggg tcaccatcct cgtctggtcc
     3481 ttccgtgcca gccggtacag gccatagaag gtggcggggt gcttgcccgc gtgcgtctgg
     3541 agccacttct cgaacctgaa aacggcgggg cgttcttccg aataggggct ggatctcttc
     3601 tcgttttgca tcgccagaat gttagcactt cgtggcaaga gatccgtctc cttgcggcgg
     3661 tatactcctc tgtactgggg ttctgtgccg atgttgagag atatttccaa cttcaagttg
     3721 ttgcggcgcc tggcgagcgt cctggcgttg ttgctggccg atgttgccgc tctactgctc
     3781 gggctcgggg tctcggcctg ggccctggac ggtcggtcgg accccgccat cggtctcgcg
     3841 cccctgctgg tggcggcctg ggtagtgctc ttcaccgcgt tccggttgta cgacagggcg
     3901 ccggtgcggc gcagcccggg ggggctcgtc ggggccgcgt tctgctgggc ggggctggtc
     3961 atcctggggg gggcgatcta ccccgagacc ggcctcggac ccgcggaaac catgttcgtt
     4021 gcctccctgg ccctcctctg tgccggctca ctcaggtacc tctacgagcg ggtcctggaa
     4081 aggatctacc gccgcggctt cggccggacg ccggttctgg tgatcggacc ccaggaggaa
     4141 agggcgcgct tgcgccgcat gatggatcgt gcggccggcg cctacgtgct ggccggcgag
     4201 ttggacatgg gggagggggg ggtcgacctc gcgtccctga gagagaagct ggacagcaca
     4261 ggggccgacg gcgttgtcct catgggggcc gagcgcttgc ccgacgacca cctcctggac
     4321 ctggtccact cggtgcggct gcgcgggata cccctgaggg tggtgcctgg cgcgttcgcc
     4381 ctcatgcgcg gacgcactat cctctcggat ggcatgggcc taccacttct ggaggtccat
     4441 tatcccggcc tggacaacac ccagcgcacc ctcaagcgcg tcctcgacgt ggccggctct
     4501 ctcggcgggc tactgatcct ctcgccgctg ctcctggccg tcgccgtcgc cgtgaggctc
     4561 gattccccgg gccccgtcct cttcaggcag aagcgcgtcg gggccgacga gaaggtcttt
     4621 atctgctaca tgttccgctc catgtacagg gatgccgagt tacgccaggc cgcgctcgaa
     4681 gacctcaacg aggccgaagg ccccgtcttc aagataagag acgatcctcg catcaccagg
     4741 gtgggacgct tcctccggcg ctggagcata gacgagctgc cccaactgat taacgtgctc
     4801 aagggcgaga tgagcctggt aggcccgaga ccactacccg taagagactt cctcaagatg
     4861 gaagaggctc acaaacgccg tctcggcgcc gtcccaggca tgaccggcta ctggcaaacc
     4921 agcggccgca gcgacctctc cttcgaagag atggtgcgcc tcgacctcta ctacatagaa
     4981 aactggtccc tctccttcga cctgaagata atcctgaaaa ccctaggcgc cgtcctgaac
     5041 cgtaa
//
LOCUS       DBNGVD010000029         5401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4741212, whole genome shotgun sequence.
ACCESSION   DBNGVD010000029 DBNGVD010000000
VERSION     DBNGVD010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4741212"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16CM_00755"
     CDS             <1..419
                     /locus_tag="AC16CM_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3138385.1"
                     /translation="DHMSEMTRMREYAEGGGGAEDQRRTHATAWIPTADIFAKGQDLV
                     IRCELAGVEREDVEVSLAGGVLTIDGERKGEPEDVSYYSRERYYGRFRRTMNLPEGVE
                     SDRITADFENGLLEVTVKGGADYPEPERIRIGTNVG"
     gene            613..1173
                     /gene="folE"
                     /locus_tag="AC16CM_00760"
     CDS             613..1173
                     /gene="folE"
                     /locus_tag="AC16CM_00760"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3138386.1"
                     /translation="MDKPRIERAVKEILLAIGEDPGREGLQDTPRRVAEAYAMLFAGL
                     GEDPVRHLDVGFRENHREMVLVRDIPLHSLCEHHLLPMVGKAHIGYIPDGKVVGLSKL
                     ARLVEGYARRPQLQERLTSQIADALNDDLGALGAIVVIEASHMCMTIRGVQKPGSMAI
                     TSAVRGTYATDQRTRQEALSLITAHR"
     gene            1552..3999
                     /locus_tag="AC16CM_00765"
     CDS             1552..3999
                     /locus_tag="AC16CM_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3138387.1"
                     /translation="MESRARVVIVGAGIVGCSVAEHLSRLGWRDVVVLDQGPLFEAGG
                     STSHAPGLMFQTNPSKMMTDLAKYSVDRYVELEADGKPCGYRSGSIEVAETPERLADL
                     ERRHGLAASWGLESHLISPRECAEKVPLLDGDRILGGFFVPSDGIAKGVRACEAMGRL
                     ASERGVKFYGQTEVTGIEVANGRVRAVETDQGRIEAELVVSCAGMWGPYIGRMVGMTV
                     PLMPMQHQFAWTTPLAELAGETREVMQPMLRHQDFSMYMRQREDYFGIGAYRHRPMPI
                     AAEDIRRDYRGEGEFPSVLEFTPEDFEPTWQEARKMIPALQNVEMARPMNGLFSFTPD
                     GGPLLGESKEARGFWIAEAVWITHAIGVGKVMAEWLIDGVPSIDLAGADVHRFEPYAK
                     SPAYILARSSQSFREVYDIIHPQQPMEDPRPLRTSPYYPRQKDLGAYFLEASGWERPQ
                     WFEANQRLLDGSDDVPGRGEWAGRYWSEIAAAEAEATRERVALYDMTALKRAEVTGPG
                     AMEFLQHLTTGRIDRPPGKVTYTLMLDEAAGVKSDITVARLEEERFQLGLNGPRDIEW
                     MERHLPSDGSVQVRDITPGTCCIGVWGPSARDVVQSLSDEDFSNEAFGFFNARYAYLR
                     EVPVTALRLSYVGELGWEIYTTSDMGLRLWDLLWDAGQPYGVVAGGRAAFNSMRIEKG
                     YRSWGTDMTTEHDPYEAGLGFAVKPDKGDFIGREALARRREAGPRRKLVCTVLDDPGV
                     VVMGYEPVFVDGRAVGYVTSADYGYTLGQSIAYAWVPPEHAEVGSKVEIQYFQKRYPA
                     TVTEDPLFDPAMKRMRA"
     gene            4099..5304
                     /locus_tag="AC16CM_00770"
     CDS             4099..5304
                     /locus_tag="AC16CM_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3138388.1"
                     /translation="MAEHINPDPGILQYTRLFKSPYFYGSRRHNVQKYSVYNHHYHPR
                     FYRDPVEEYYQLLEGVTMWDVGGAERQVEITGPDAFEFTNMLTPRDLNKCNVEQCKYA
                     FITNAQGGIINDPVLLRLEENHFWLSLADSDVLLWAQGLAYNMGLDVEITEPDVGPMQ
                     VQGPKSKEVMTDLFGEDILDVPYYYMVHRQLDGMELVISRTGYSGELGYELYLKNASR
                     DGLKLWDRVLQAGEPHGISPIGPCHIRRIEGGILALGCDMWYDTNPYEVGYGYKWMVE
                     LEQEQDFVGKEALHKVHEEGVSRKLVGVEIGGSNCGTYNDGSMIDYFPVHKNGERIGK
                     VTSACYSPRLEKNIGFAMVPVEYSEIGIDLEVERPEETVSATVADRIFFKPEHAEQKL
                     TATSSNEGA"
BASE COUNT         1059 a         1633 c         1825 g          884 t
ORIGIN      
        1 tggaccacat gagcgagatg accaggatgc gggagtatgc cgaaggtggc ggtggggcgg
       61 aagaccagcg caggacccac gcgacagcct ggatcccgac ggccgacatt tttgccaagg
      121 gtcaggatct ggtgatacgc tgcgagctcg caggggtgga gcgggaagat gtggaggtct
      181 ccctcgccgg cggcgtgctg accatcgacg gggagcgcaa gggagagccg gaggacgtta
      241 gctactactc cagggaacgc tactacgggc gtttccggcg gaccatgaac ctgccggagg
      301 gcgtggagag cgacaggatc accgccgact tcgagaacgg tctgctggag gtgaccgtga
      361 agggcggggc cgattacccc gagcccgagc gtatccggat aggaaccaac gtaggttagg
      421 caaccctgct agagaaatac gccaacaccg ggcccggccg tgagggggag cagggcccgg
      481 tgttttatct gaagcggctc cgaatcgacg ggactcgccg taccttcttc cgagacggga
      541 tgcgtgccgc agcacgattc ttgtatgatg gctccatgac gaaagaggca tcacgggacc
      601 ggggcatgtc gcatggataa gcctcgtatc gagcgggccg tcaaagagat actgcttgcc
      661 ataggggagg accccggccg cgagggtttg caggacacgc cccgacgggt cgcggaggcc
      721 tacgcgatgc tcttcgccgg tctgggggag gaccccgttc ggcatctgga tgtcggcttt
      781 cgagagaacc accgcgagat ggttctggtc agggatatac ccctgcactc cctctgcgaa
      841 catcaccttc tgcctatggt gggcaaggcc cacatcggct acatcccgga cggaaaggtg
      901 gtcgggctgt ccaagctggc ccggctggtg gagggctacg cccgccgccc ccagctccag
      961 gagcgcctca cctcccagat cgccgacgcc ctgaacgacg acctgggagc cctcggcgcc
     1021 atagtcgtga tcgaagccag ccacatgtgc atgacgatca ggggcgtcca gaaaccggga
     1081 agcatggcca tcaccagcgc ggtgcgcgga acctacgcaa cggaccaacg cacccgccag
     1141 gaagccttga gccttataac ggctcatcgc tgaagcgatc cgcgaaacag gagaactcga
     1201 gaaaaggcat caggctacag gggtatagca gtgcgtcaga agcccgacct cgcggagcgg
     1261 gaagccttac gccagagata ggatactgac agctgataac tgatggctga cagctgacgg
     1321 ctgtcagctg ccaaagcccg aagcctctcc cataatcgca ctggctgaca ccttcccacc
     1381 ccctgaagcc cctctagcgg gccgcaagaa cttcgacccc actgccttct tgactctagt
     1441 tccgtcgtgt gttacttgtg ccgtatacag gaaaggattt cgccatgcgc aacaaacctt
     1501 caacaatttt gtatggcgct cgcggtacgg cgggggtgtt tggggtcgtg aatggagagt
     1561 agggcccggg tcgtgatcgt gggcgccggc atcgtcggct gcagcgtggc cgagcacttg
     1621 agccggctcg gctggcggga cgtggtggtc ctggatcagg ggccgctctt cgaggctggt
     1681 gggtccacct cccacgcccc ggggctcatg ttccagacca acccctccaa gatgatgacc
     1741 gacctggcca aatacagcgt cgaccgctat gtggagctcg aggctgacgg caagccgtgt
     1801 ggctacagga gcggcagcat agaagttgcg gagaccccag agcgattggc cgacctcgag
     1861 cgcaggcacg ggctcgccgc ctcctggggt ctcgagtcgc acctcatctc tccccgggag
     1921 tgcgcggaga aggtgccgtt actggacggt gacaggatcc tcggtggctt cttcgttcca
     1981 tccgacggta tcgccaaggg cgtcagggct tgcgaggcga tgggacggct ggcgtccgag
     2041 cgtggcgtca agttttacgg ccagaccgag gtaaccggca tcgaggtcgc gaacggcagg
     2101 gtcagggcgg tggagaccga ccaggggcgc atcgaggcgg agctggtcgt cagctgcgcc
     2161 gggatgtggg ggccatacat cgggcggatg gtcgggatga ccgtgccgct catgccgatg
     2221 cagcaccagt tcgcgtggac cacgccgctc gccgagcttg ccggggagac ccgtgaggtc
     2281 atgcagccca tgctgcgcca ccaggacttc tccatgtaca tgcgccagag ggaagattac
     2341 ttcggcatcg gggcttacag gcaccgtccc atgcccatcg ccgccgagga tatcaggcga
     2401 gattacaggg gcgagggcga gtttccgtcg gtgctcgagt tcacgccgga ggacttcgag
     2461 ccgacctggc aggaggcgcg gaagatgata cccgcgctcc agaacgtcga gatggcccgt
     2521 cccatgaacg ggctgttctc cttcacgccc gatggcgggc cgttgctcgg cgagtcgaag
     2581 gaggcaagag ggttctggat cgctgaggcg gtctggatca cgcacgccat cggcgtgggc
     2641 aaggtgatgg ccgagtggct tatcgatggc gtcccgagca tagacctggc cggggccgac
     2701 gttcaccgct tcgagcccta cgccaagagc ccggcctaca tcctggcccg ctcctcgcag
     2761 tccttccgcg aggtctatga catcatccat ccccagcagc cgatggagga cccacgcccg
     2821 ctccggacca gcccgtacta cccgcggcag aaggatctgg gtgcgtactt tctggaggcc
     2881 tccgggtggg agcgcccgca atggttcgag gcgaaccagc ggctactcga cggcagtgac
     2941 gatgttccgg gacgtgggga gtgggccggg cgatactggt ccgagatagc cgccgccgag
     3001 gcagaggcaa cccgcgagcg ggtcgccctc tacgacatga cggcgctcaa gcgggccgag
     3061 gtaaccgggc cgggcgcgat ggagttcctg cagcatctga ctacgggacg gatagatcgt
     3121 ccgcctggca aggtgactta cacgctcatg ctggacgagg ccgccggggt gaagagcgac
     3181 atcaccgtcg cccgcctgga ggaagagcgc ttccagctcg ggctcaacgg gccaagggac
     3241 atcgagtgga tggagcgcca cctgccgtcc gatggctccg tgcaggtgcg ggacatcacg
     3301 ccggggacct gctgcatcgg ggtctggggg ccctctgccc gcgacgtggt gcagagcctc
     3361 tccgatgagg acttctccaa cgaggccttc gggttcttca acgccaggta cgcctacctg
     3421 cgcgaggttc cggtaacggc cctcaggctc tcctacgtcg gtgagctcgg gtgggagatc
     3481 tacaccacct cggacatggg gctgcggctg tgggatttgc tgtgggacgc cgggcaaccg
     3541 tacggcgtcg tggccggtgg ccgcgccgcc ttcaactcca tgcgcatcga gaagggttac
     3601 cgctcctggg gcacggacat gacgaccgag cacgacccgt acgaggccgg cctcggcttc
     3661 gcggtcaagc cagacaaggg agacttcatc gggcgcgagg cgttggccag acgccgggag
     3721 gccggaccgc gccgcaagct ggtttgcacg gtgctcgacg atccgggcgt ggtcgtgatg
     3781 ggctacgagc cggtgttcgt tgacggccgt gcggtcgggt acgtgaccag cgcggactac
     3841 gggtacacgc tcgggcagag catagcgtac gcgtgggtcc cgcccgagca cgcagaagtc
     3901 ggtagcaagg tggagatcca gtacttccag aagcgctatc cggcaacggt gaccgaagat
     3961 ccgctcttcg atcccgcgat gaagcggatg cgggcgtagg tgatgcgaag tacgccgtag
     4021 gggaagaccg ggatcttccc agaagattag atgttggatt gaagattcag agacaccaac
     4081 agaaaggaaa cacgccccat ggccgaacac atcaatccgg atccgggcat cctgcagtac
     4141 accaggttgt tcaagtcacc ttacttctac ggctcgcgcc ggcacaacgt ccagaagtac
     4201 agcgtgtaca accaccacta tcacccgcgc ttctatcgcg accccgtaga ggagtactac
     4261 cagcttctcg aaggcgtcac gatgtgggac gtgggcggcg ccgagcggca ggtcgagatc
     4321 accggcccgg acgcgttcga gttcaccaac atgctcacgc cgcgtgacct gaacaagtgc
     4381 aacgtggagc agtgcaagta cgccttcatc actaacgccc agggcggcat aatcaacgac
     4441 ccggtgctct tgcggctcga ggagaaccac ttctggctct cgctggcgga tagcgacgtg
     4501 ctgctctggg cgcaggggct cgcctacaac atgggcctcg atgtggagat cacggagccg
     4561 gacgtcggcc cgatgcaggt gcagggcccg aagtcaaagg aggtcatgac ggatctcttc
     4621 ggcgaggaca tcctggatgt cccctattac tacatggtgc accggcagct cgacggcatg
     4681 gagctggtga tctcacgcac cggctactcg ggcgagctcg gctacgaact ctacctcaag
     4741 aacgccagcc gcgacggcct gaagctctgg gaccgggtgc tgcaggctgg cgagccgcac
     4801 ggtatctccc cgatcggacc ctgccatata cgcaggatcg agggcggcat cctggccctt
     4861 ggttgcgaca tgtggtacga caccaacccc tacgaggtcg gctacggcta caaatggatg
     4921 gtcgagctgg agcaggagca ggacttcgtc ggaaaggaag ccctgcacaa ggttcacgaa
     4981 gagggtgtca gccgcaagct cgtcggcgtc gagatcggcg gcagcaactg tgggacgtac
     5041 aatgacggtt ctatgatcga ctacttcccg gttcacaaga acggtgagag gatcggcaag
     5101 gtcacctcgg cctgctactc cccgcgcctg gagaagaata tcgggttcgc catggtgccc
     5161 gtcgagtaca gcgagatcgg catcgatctc gaggtcgagc gcccggagga gacggtctct
     5221 gctacggtgg ccgaccgcat cttcttcaag ccggagcacg ccgagcagaa gctcaccgcc
     5281 acgagctcta acgaaggcgc ctagcggaga gcagcggcaa caggggccgc cgcgcaggtt
     5341 cggcgcggtg gcccgttgac ccccaagcga taagaggacc gtgtgatgca ggacaggagg
     5401 a
//
LOCUS       DBNGVD010000030         5727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3830374, whole genome shotgun sequence.
ACCESSION   DBNGVD010000030 DBNGVD010000000
VERSION     DBNGVD010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3830374"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="aroA"
                     /locus_tag="AC16CM_00775"
     CDS             <1..1022
                     /gene="aroA"
                     /locus_tag="AC16CM_00775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3138389.1"
                     /translation="IPASDLDIFVGNAGTVARFLPPALALGPGPYTVDGVQRMRERPI
                     SDLVEGMRRLGAAVDYAGEPERYPLKVHGGGIEGGTAHIPGARSSQFASGLLMAAPYA
                     RTPVTLRVDGREQWPYIGITVSVMRSFGVDVTEAPDLFSVQPAKYRPRNYAVEPDASA
                     ASYFLAAAAVTGGTVEVPGLGSTSTQGDLRFAEVLQKMGCDLHMTPDSIRIQGPARLQ
                     GVDVDMNAFSDTMMTLAAIAPFASGPTTIRNVSHTRHQETDRLSAVATELGRLGIRTD
                     QTSSSIRIIPQTPRPAVVRTYGDHRMAMAFAVTGLVAPGISIRDPGCVTKTFPDFFRR
                     LEELR"
     gene            1055..1714
                     /gene="cmk"
                     /locus_tag="AC16CM_00780"
     CDS             1055..1714
                     /gene="cmk"
                     /locus_tag="AC16CM_00780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3138390.1"
                     /translation="MGGILVTIDGPAGSGKSSVARAVADRLGVVNLNTGAAYRAAALL
                     ALRENVDLEDGPGLARLAGRVELDPEGASIDGEMVAEPDLRTPEVSAAASRVSAHGQL
                     REVLLPVQRAAAQKARERGGAVVEGRDIGTVVLPDAELKVYLSAAPEERAWRRATQTG
                     REDELERIRAAIARRDRQDSEREVSPLRPAEGAVVLDTTSLSLEEVISEVLRLAGERE
                     G"
     gene            1711..2367
                     /locus_tag="AC16CM_00785"
     CDS             1711..2367
                     /locus_tag="AC16CM_00785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3138391.1"
                     /translation="MSGALRLFRGRQKPGEMTPRYRLFRWSMIRLCRLLFGYTLHGEE
                     RVPPEGPLVIASNHGQYVDPVLVCVAVPRRVQWMGKKQLFVFPFKTFFHFIGTFPVDR
                     EGGGRGAIRAALAFLGEGWALGIFPEGTHRGDEGSKDAKSGVVVLASRGKAPVLPVYV
                     GRLPGITGRLRGGRLHVYIGEPIRLDPALRGGAAYRAQAEEIMQTIYALPRAAEAGSL
                     "
     gene            2364..3668
                     /gene="der"
                     /locus_tag="AC16CM_00790"
     CDS             2364..3668
                     /gene="der"
                     /locus_tag="AC16CM_00790"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3138392.1"
                     /translation="MKRLPVVAVVGAPNVGKSSLVNRILRRRGRVVSEQPGTTRDRSY
                     SRAEWAGRDFILVDTGGMEPGARVGLEALVTLQARVAVEEADVVLHLTDARLGPTEAD
                     GAIARELRRARGKSVLAVNKLDNPADDSERHRFYSLGEGEPHAISAIHGLGVGDLLDV
                     VVSMLPEYEPEDEQELPRLAIVGRPNVGKSTLLNRLLGAERAVVSEEAGTTTDAVAGE
                     IEIPLDGGLERYVLLDTAGVSKGARRAQGVPYYSSLRTSEAIRRSDVSLLLVDAVDGI
                     VAGDLRIAREIEGAGSSCGVVVNKRDLVPPERLQEVEGLISARLTDLKPRTVRISALT
                     GAGVDAVLPFAAAVGRAYRLRVPTHEVAEFVGEVVARNAPPKGVKIRYATQTGGSPPR
                     FTLFANRPKDLPENYLRYVENALRERYDLWGVSVRIKVKSSR"
     gene            3732..4025
                     /locus_tag="AC16CM_00795"
     CDS             3732..4025
                     /locus_tag="AC16CM_00795"
                     /inference="COORDINATES: protein motif:HMM:NF046924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiG family protein"
                     /protein_id="HIR3138393.1"
                     /translation="MEDSGVLRGFDWEEAEDITALSEEELGDLLQDLIGEERTVSYRR
                     RVLQSRIDLIRAELAGRDRDPAMPALAPEELARVLLGNPIGNSLGDTYPEERP"
     gene            4022..4468
                     /locus_tag="AC16CM_00800"
     CDS             4022..4468
                     /locus_tag="AC16CM_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3138394.1"
                     /translation="MRTCPECGSDVAPGVRFCSECGSRLEVGQSTVSYAPSFGEEVEP
                     TSGGADGAALIEMDQVEGTAGRRMHDLGDQVVTVGRGQESDIFLDDVTVSRRHAEIVQ
                     GGAGYRIRDVGSLNGTYVNRVRVDAVDLRNGDEIQVGKYRFRFVFS"
     gene            4503..5225
                     /gene="ftsR"
                     /locus_tag="AC16CM_00805"
     CDS             4503..5225
                     /gene="ftsR"
                     /locus_tag="AC16CM_00805"
                     /inference="COORDINATES: protein motif:HMM:NF050798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtsR"
                     /protein_id="HIR3138395.1"
                     /translation="MENDGSMDNRMDEDFGPPGERYSQTKDSFTIGEVVERLKPEFPS
                     LSVSKIRYLERRRLINLSRTRGGYRLFSSQDIELLRYILTLQDKEYLPLKVIKKRIEG
                     GAAFAGGDSAGDLSRVLEDRTYTREELADTLETEIRFVEELISVGVIGRSGELTQRDA
                     EIARMALEMAKYSIQPRHLRGIMAAVDREAALFKQVVNPELRSGDDGRVGEAMTKARH
                     LASVDSRMKELMMANVIDTFAP"
     gene            5222..>5727
                     /locus_tag="AC16CM_00810"
     CDS             5222..>5727
                     /locus_tag="AC16CM_00810"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3138396.1"
                     /translation="MRDPALANAAREIEAHIRTVPDFPRAGVSFKDITTLIEDGRALH
                     TAVDALAAATEGIEYDRILSAEARGFVFGTALAYRAKKGLILARKPNKLPRDTISATY
                     ELEYGTDALEAHTDSIPEGTRVLVADDLLATGGTARAMCQLVENAGGTVAGVAFLIEL
                     AYLSGRDQL"
BASE COUNT         1027 a         1877 c         1937 g          886 t
ORIGIN      
        1 ggatacccgc ctccgacctg gacatcttcg tcggcaacgc cggcacggtg gcccgcttct
       61 tgccgccggc gctggccctc ggccctggcc cctacaccgt ggacggcgta cagaggatgc
      121 gggaacggcc catatccgac ctggtagaag ggatgcgccg cctcggggcc gcggttgact
      181 acgccggcga accggagaga tacccgctca aggtgcacgg cggcggcatc gagggcggga
      241 cggcgcacat cccaggggcc agaagcagcc agttcgcgag cggcctcttg atggcggctc
      301 cctacgccag gacccccgtt accctcaggg tcgacgggag agagcagtgg ccctacatag
      361 gcattacggt ctctgtcatg cgctccttcg gcgtcgatgt aaccgaggcc cccgacctct
      421 tctccgtcca accggcaaag tacagacccc gcaactacgc ggtcgagccg gacgcctcgg
      481 cggcctccta cttcctggcc gcggcggcgg tcaccggcgg aaccgttgaa gtccccggcc
      541 tgggctccac ttccacccag ggagacctgc gcttcgcaga agtcctccag aagatgggct
      601 gcgacctcca tatgaccccc gactccatcc gcatacaggg cccggcccgc ctgcagggtg
      661 tggacgtcga catgaacgcc ttctccgaca ccatgatgac cctggccgcc atcgccccct
      721 tcgcctccgg cccaaccacc ataagaaacg tatcccacac ccgacaccag gaaaccgacc
      781 gcctctccgc cgtcgccacc gaactcggcc gcctcggcat aagaacagac cagacctcct
      841 cctctatccg cataataccc caaactccac ggcctgccgt ggtacggact tacggcgatc
      901 acaggatggc catggcgttt gctgtcacgg ggcttgtagc gccagggatc tcgatccggg
      961 accctgggtg tgttaccaag acgttcccgg attttttccg gcggttggaa gaacttcggt
     1021 aggacgcggg ctttcggata tactcccggt accgatggga ggcattctcg taacaataga
     1081 cgggcctgcc gggagcggga agagctcggt cgcgcgggcg gtcgcggacc gcctcggggt
     1141 cgtgaacctc aacacgggcg ccgcatacag ggcggcggcc ctgcttgccc tgcgtgagaa
     1201 cgtcgacctc gaggacgggc cggggctcgc gcgcctcgcc ggcagggtcg agctggaccc
     1261 ggagggcgcg agcatcgacg gggagatggt agccgagccg gacctgagga cccccgaggt
     1321 ctcggccgcc gcctcccggg tgtcggcgca cgggcagtta agggaggtgt tgctccccgt
     1381 gcagcgggca gccgcccaga aggccaggga gcgtggtggg gccgtcgtgg agggccggga
     1441 tatcgggacc gtcgtcctcc ctgacgctga gctaaaggtt tatctgtcgg ccgcgcccga
     1501 ggagcgggcc tggcgccggg cgacgcagac gggcagggag gacgagttgg aacgcatcag
     1561 ggccgccatc gccaggcgcg accgccagga ctccgagcgg gaggtctcac cgctgaggcc
     1621 agccgagggc gccgtggtgc ttgacaccac ctccctcagc ctcgaagagg tgatctccga
     1681 ggtcttgcgg ctcgccgggg agcgggaagg gtgagcgggg cgttgcggct gttcaggggc
     1741 aggcaaaagc cgggtgagat gaccccccgc taccggctct ttcggtggtc catgatccgg
     1801 ctctgccgcc tgctcttcgg atataccttg cacggtgaag agcgggttcc ccccgagggc
     1861 cccctggtta tagcctcgaa ccacggccag tacgtcgatc ccgtgctggt ctgcgtggcc
     1921 gtgccccggc gggtgcagtg gatgggcaag aagcagctct tcgtcttccc gttcaagacg
     1981 ttcttccact tcatagggac attccccgtg gaccgcgagg gtgggggaag gggcgccata
     2041 cgcgccgcgc tcgcgtttct gggcgagggc tgggcccttg ggatctttcc ggagggtacc
     2101 cacaggggcg atgagggatc caaggacgca aagagcgggg tcgtcgtgct cgcctcgcgt
     2161 ggtaaagccc ccgtgctgcc cgtctacgtc ggtcggctgc ctgggataac cggcaggcta
     2221 cgcggggggc ggctgcacgt ctacatcggg gagcccatac ggctggatcc ggcgctcagg
     2281 ggcggggctg cctaccgggc gcaggccgag gagataatgc aaaccatcta cgcgctgccc
     2341 agggccgcag aggcgggttc cttatgaaaa gacttcccgt ggtcgccgtc gtcggggcgc
     2401 caaacgtcgg caagagctcg cttgtgaaca ggatcctgcg ccgccgcggc cgcgtggtct
     2461 ccgagcagcc gggaaccacg cgcgacaggt cctacagccg ggccgaatgg gccggccgcg
     2521 acttcattct ggtcgacacc ggggggatgg agccgggggc cagggtgggc ctcgaggccc
     2581 tcgtgacgct gcaggcgagg gtcgccgtcg aggaggccga cgtcgtgctc cacctcaccg
     2641 acgccaggct cggaccaaca gaggccgacg gtgctatcgc ccgcgagctg aggcgtgcca
     2701 ggggcaagtc tgtgctcgcc gtcaacaagc tggacaaccc cgccgacgac tctgagcgcc
     2761 acaggttcta ctcccttggc gagggcgagc cgcacgccat ctccgccatc cacggcctcg
     2821 gcgtaggaga cctgctcgac gtggtcgtct ccatgttgcc cgagtacgag ccggaggatg
     2881 agcaggagct tccgaggttg gccatcgtgg gcaggccaaa cgtcggcaag agcaccctcc
     2941 tgaaccggct tctgggcgcg gagcgggccg tggtctctga ggaggccggc accaccaccg
     3001 acgccgtggc cggggagatc gagataccac tcgatggcgg cctggagcgt tacgtcctgc
     3061 tcgacaccgc cggcgtcagc aagggggcga ggcgtgcgca gggtgtgccc tactactcct
     3121 cgctccggac ctccgaggcc atccggcgct ccgacgtctc actgctcctg gtcgacgccg
     3181 tagacggcat cgtggccggg gacctcagga tcgcgcgcga gatagagggg gccggctcct
     3241 cgtgcggggt ggtggtgaac aagcgggacc tcgtacctcc cgagaggcta caggaggtgg
     3301 agggactgat ctcggcccgt cttacagacc tgaagcccag gaccgtgcgg atctccgccc
     3361 tgaccggcgc cggcgtcgac gcggtcttgc ccttcgcggc tgccgtgggg cgggcgtacc
     3421 ggctcagggt gcccactcac gaggttgccg agttcgtggg cgaggtagtt gcgcgcaatg
     3481 cgccgcccaa aggggtgaag atccgctacg ctacccagac cggggggtcc cctccgaggt
     3541 tcaccctctt cgccaacaga ccaaaagacc tccccgagaa ctacctgcgc tacgtagaga
     3601 acgccctgag ggagcgctac gacctgtggg gcgtcagcgt gaggataaag gtcaagagca
     3661 gcaggtagga ccacataaat gctctcaagc taagattgca cttgaggttc agggcgggtt
     3721 atactccttg catggaagat tcgggggtgc tccggggttt cgactgggag gaagcggagg
     3781 acattaccgc gctctcggaa gaggaactcg gggacctatt gcaggatctg atcggggagg
     3841 agaggaccgt aagctacagg cgccgcgttc tgcagagcag gatagatctc atcagggccg
     3901 agcttgcagg ccgcgaccgt gacccggcca tgcccgcgct cgcgcccgaa gaattggccc
     3961 gggttctgct cgggaacccg atcggaaact cgctcgggga tacatacccc gaggagcggc
     4021 catgaggacg tgtcccgagt gcgggtccga cgtagccccc ggggtcaggt tctgctccga
     4081 gtgcggcagc aggttggagg tcgggcagag cacggtctcc tacgccccga gcttcggtga
     4141 ggaggtggag cccacctccg ggggagccga cggggcagcc ctgatcgaga tggatcaggt
     4201 ggagggcacg gcgggccgca ggatgcacga tctgggagac caggtcgtaa ccgtcggacg
     4261 cgggcaggag agcgacatct ttctggacga cgtcaccgta tcgagaaggc acgccgagat
     4321 cgtgcaaggc ggcgcaggct acaggatacg cgacgtcggc agcctgaacg ggacctacgt
     4381 caaccgcgtt cgcgttgacg ccgtcgacct tagaaatggg gacgagatac aggtcggcaa
     4441 gtaccgcttc aggttcgtgt tctcctagta cagcgacaac cactgacgat gggaggcaga
     4501 gggtggagaa cgacggcagc atggacaata ggatggatga ggacttcggg ccccctggcg
     4561 agaggtatag tcagaccaag gacagcttca ccataggtga ggtcgtcgag cgcctgaagc
     4621 ccgagttccc aagcctctcg gtcagcaaga tccggtacct cgaacggcgg cgtctgatca
     4681 acctgagccg aacgcgcggc ggctaccggc ttttctcctc acaagacatc gagctgctgc
     4741 gctacatcct caccctgcag gacaaagagt acctccccct gaaggtgatc aagaagcgta
     4801 tcgagggcgg cgcggccttc gccgggggcg actcggccgg cgacctctcg cgcgtgctgg
     4861 aggacaggac ctacacccgc gaggagctcg ccgataccct cgagaccgag atccgtttcg
     4921 tagaagagct tattagcgtc ggcgtgatag gccgaagcgg cgagctgacc cagcgcgacg
     4981 ctgagatagc gcgcatggcc ctcgagatgg cgaagtactc catccaaccc cgacatttgc
     5041 gtggcatcat ggccgccgtg gaccgcgagg cagccctctt caagcaggtg gtgaaccctg
     5101 agctgaggag tggcgacgac ggccgtgtgg gggaggccat gaccaaggcc cgccacctcg
     5161 cctcggtcga ctcccgcatg aaagagctga tgatggcaaa cgttatagac actttcgccc
     5221 cgtgagggac ccggcgctgg ccaacgccgc cagggagatc gaagcccaca tccgcacggt
     5281 accggacttc ccacgcgccg gcgtctcctt caaggacatc acgaccctga tcgaggacgg
     5341 acgcgccctg cataccgccg tcgacgccct ggccgcggcc accgagggca tcgaatacga
     5401 ccgcatcctc tccgcagagg cccgcggctt cgttttcggc accgccctgg cctaccgcgc
     5461 caagaaaggc ctcatcctcg cccgcaaacc aaacaagctg ccgcgggata ccatctcggc
     5521 cacctacgag ctggagtacg gcacggatgc cctcgaagcc cataccgact ccatcccgga
     5581 gggaacccgc gtcctcgtgg ccgacgatct cctggctacc ggcggcacag cccgcgccat
     5641 gtgccagctt gtcgagaacg ccggcggcac cgtcgctggc gtcgccttcc tcatagaact
     5701 cgcctacctc tccggccgcg accagct
//
LOCUS       DBNGVD010000031         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1826502, whole genome shotgun sequence.
ACCESSION   DBNGVD010000031 DBNGVD010000000
VERSION     DBNGVD010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1826502"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..757)
                     /locus_tag="AC16CM_00815"
     CDS             complement(260..757)
                     /locus_tag="AC16CM_00815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138397.1"
                     /translation="MSAHEPEDVRVVHLEGARFREYRERLVRDYAADKVRAGVWPEDE
                     AEDRAARELDGLLPDGPLTRDHYLYSVRDESDQTEVGYLWISPRDSGAGRALWIYDIV
                     VHEEYRRRGYAERMLHLAEDRARELGAGRVELHVFGHNKAARALYEKVGYTPTDIIMA
                     KPVDD"
     gene            complement(762..1685)
                     /gene="holA"
                     /locus_tag="AC16CM_00820"
     CDS             complement(762..1685)
                     /gene="holA"
                     /locus_tag="AC16CM_00820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3138398.1"
                     /translation="MAVYLLLGDDDDRKSRGVEKLRKGRNVEAFDASVTGPDGIVSAC
                     NSFSLFGEGPFVVLRNLDAWNAAQKAVIVDYLQNPSPGSDLILIGTKLGARERLLSAV
                     KKSGEVHTFEQPTGKALVRWLVGHARKRGLDLPEDVAEDLTTRCSEDKMRLLQETEKL
                     ALYVGDGQATHDDVAALCPPDVQSNIFAFVDALAAGQRDRALWLLENLISTGEPPLRL
                     TFMIRRQFQLVARARALLDRGTPQREIASVLKVPPFVARKLDEQGRKLGDEDLDIALA
                     AIRDLEGGLKGGSNLPDDFQVELAVLKLSRA"
     gene            complement(1758..2687)
                     /locus_tag="AC16CM_00825"
     CDS             complement(1758..2687)
                     /locus_tag="AC16CM_00825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3138399.1"
                     /translation="MDYGVHLPLIGFGDRPPAYRHLIRYTETAERLGFTALAVNDHLL
                     FSRPWLDGPTALSAVLEATGRMDLMTTVALPVVRGPIALAKSLAAIDILSGGRLIAGL
                     GPGSSERDYTAVDIPFDERFKRLDEAVGTLRALWSRDVAPFRGAYYSTAGVDLEPRPA
                     RSGPPIWIGSWGSEAGLRRTARLGDGWLASAYNTTPAEFDDARARLGEHLAAAGKDPD
                     RFPNAIATMFLHTTEDPLQADQICDTVLGPMLRRPPGQLRERLLVGHPEECAEKLVAL
                     RAAGARCILLWPVWNELHQLEMFRERVVPLVDV"
     gene            complement(2831..>3046)
                     /locus_tag="AC16CM_00830"
     CDS             complement(2831..>3046)
                     /locus_tag="AC16CM_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3138400.1"
                     /translation="RLEDNLNPVGRIFYSASTTICVPNSRDQEVGLALGAQAGEQRVR
                     EVVTAGGFSSFRRVAETPFNLVYEARP"
BASE COUNT          512 a          968 c         1012 g          554 t
ORIGIN      
        1 cagcgcgccc ccgggttcga cgttccagac tttctcgccg ccggtcccag acacttgctg
       61 gtgcccggcg cttacctgga ggaggccagg cagatcctcg aggacacaac ggggctcggg
      121 tcttacgtgc cttagagagc tttgctagag gcctacggtc ttcaagggcg aggtgtcagc
      181 tgtcagacaa gaaaaatgag ctgatggcta tctttcgaga ggattggtcc tgcaacacgc
      241 tgccatgcga tagggatgct caatcgtcga ccggtttggc catgatgatg tcggtcgggg
      301 tatagcccac cttttcgtag agggcgcgcg ctgccttgtt gtggccgaag acgtgaagct
      361 ctaccctgcc agcgccaagc tccctggccc tatcttctgc gaggtgcagc atgcgctcgg
      421 cgtagcccct gcggcggtac tcttcgtgga cgactatgtc gtagatccag agcgcgcggc
      481 ccgccccgga gtctcgcggg gagatccaga ggtagccaac ctcggtctgg tcggactcgt
      541 ccctgaccga gtagaggtag tgatcccggg taagcgggcc atcgggcagt aacccgtcta
      601 gctctctcgc cgctctgtcc tcggcctcat cctcgggcca gacgccagcc cgtaccttgt
      661 cggccgcgta gtcgcgcacc aggcgctccc ggtactcgcg aaaccgtgct ccttccaggt
      721 gaacgaccct gacgtcctct ggttcatgtg cggacatcgc ctcaggcccg cgagagcttc
      781 aggacggcca gctcaacctg gaaatcgtct ggaaggttgc ttccgccttt caggccgccc
      841 tctaggtccc ggatcgcggc caacgcaatg tccaggtcct cgtcgccgag ttttctgccc
      901 tgttcgtcca gcttgcgtgc cacgaacggg ggcaccttca gtacgctcgc tatctcacgc
      961 tggggggtac cgcggtctag aagagcgcgg gcgcgggcga caagctggaa ctggcgccgg
     1021 atcatgaacg tcaaccgcag cgggggctcg ccggtggaga tcaggttctc cagcagccag
     1081 agcgccctgt cccgctgccc cgcggccagc gcgtcgacga aggcgaagat gttggactgg
     1141 acgtccggcg ggcacaatgc cgcgacgtcg tcgtgggtcg cctggccgtc accgacgtag
     1201 agcgcgagct tctccgtttc ttgcagaagg cgcatcttgt cctcagagca gcgggtcgtg
     1261 aggtcctctg ccacgtcctc gggaaggtcg aggccacgct ttctggcgtg gcccaccagc
     1321 cacctgacca gggccttgcc cgtcggctgc tcgaaggtgt ggacctcccc agacttcttg
     1381 accgccgaga gcagccgctc gcgggcgccc agcttggtgc cgatcaggat tagatcggac
     1441 cccggggacg gattctgcag gtagtcgacg atgacggctt tctgggcggc gttccaggca
     1501 tccaggttgc gcaagaccac gaacggtcct tccccgaaga gcgagaagga gttgcacgca
     1561 gaaacgatac cgtcaggacc ggtcaccgag gcgtcgaaag cctccacgtt gcggcccttg
     1621 cgcagcttct ccacgccgcg cgacttgcga tcgtcgtcgt cgcccaggag caggtagaca
     1681 gccatacacg aattctacag cagcgcatcg gcatttcagc ccggggcttc gcggctcgtc
     1741 gttcggtcag tcgatgctta cacgtctact aggggcacga cgcgttcgcg aaacatctcc
     1801 agctggtgta gctcgttcca gacgggccag agaaggatgc accgcgcgcc ggcagcgcga
     1861 agggccacga gcttctcggc gcattcttcg gggtggccta ccaggagacg ttcgcgcagt
     1921 tgtccggggg gacgtcggag catggggcca agtacggtgt cacagatctg atcggcctgt
     1981 aaagggtctt ctgtggtgtg gagaaacatg gtggcgatgg cgttcgggaa ccgatccggg
     2041 tctttgccgg cggcggcgag gtgttctccg agtcgagccc gcgcatcgtc gaactccgcg
     2101 ggggtagtgt tgtaggcgga ggccagccag ccatcgccca ggcgggccgt gcggcgaagt
     2161 ccggcctccg agccccagct gccgatccag atggggggtc ccgaccgggc tggccgcggc
     2221 tccagatcaa cgccagcggt cgagtagtac gcgcccctga acggtgctac gtctcggctc
     2281 cagagggcgc gcagcgtccc taccgcctcg tcgaggcgtt tgaaccgctc gtcgaaaggg
     2341 atgtcgacgg cggtgtagtc cctttcagag gagccgggtc ccaaccctgc tatgagacgg
     2401 ccgccggaga ggatgtctat ggcggccagg ctcttggcca gggcgatcgg tcccctgact
     2461 accgggaggg ctaccgtggt catcaggtcc atacgtccgg ttgcctcgag caccgcggag
     2521 agggccgtcg gaccgtccag ccatggtcgg gagaacagga ggtgatcgtt gaccgcaagc
     2581 gcagtgaagc cgagcctctc ggcggtctcg gtgtaccgga tcaggtgtcg gtaggcaggg
     2641 ggcctatcgc cgaagcctat caatggaaga tgtacgccgt aatccatgcg atacccacca
     2701 tcccgggacg tcttgcctgc agtgaagata tcgggggtcg gcccggaccg caacacctcg
     2761 ggcccgcgac cgggctcgcc ctcgttcccg atgagatcat gggcaccgtc tcactcatcc
     2821 gcgcctgaga ctacgggcgg gcttcgtaga ccaggttgaa tggcgtctcg gcgacccgcc
     2881 ggaagctcga gaagccgccg gcggttacca cttcgcgcac ccgctgttcc ccggcctggg
     2941 cgccgagggc gagacccacc tcctggtccc tggagttcgg cacgcagatg gtggtggagg
     3001 cggagtagaa gatcctacca accgggttga ggttgtcctc gagacg
//
LOCUS       DBNGVD010000032         5067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2373526, whole genome shotgun sequence.
ACCESSION   DBNGVD010000032 DBNGVD010000000
VERSION     DBNGVD010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2373526"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="efeB"
                     /locus_tag="AC16CM_00835"
     CDS             complement(<1..1198)
                     /gene="efeB"
                     /locus_tag="AC16CM_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HIR3138401.1"
                     /translation="MLGRKISRRGALTLAGAFGTGLTLGTGGALSARELAQADQTVEF
                     YGEHQAGIATPQQDRLHFASFDLTTSDAGEVRELMRAWSEAAATMCAGHLVGGENEDA
                     FVPPDDTGEAQDLTAGHLTITFGFGPTLFEKDGEDRFGLASRRPGALQPIPDLPGDNL
                     DPSLSGGDLCVQACADDPQIAFHAVRNLARIGRGLAVIRWSQLGFGRTSSTSSAQKTP
                     RNLMGFKDGTNNIRAEDEGAMDDYVWVREGPDWMKGGTYLVARRIRMLIEIWDRSPLL
                     EQEQTIGRHRASGAPIGKQDEFDPVDLKAKGPDGLPAIPMDSHVRLSRQNPNERILRR
                     GYSFTDGMDSKTGQLDAGLFFISFQRDPGEQFVPLQRRLGQSDRLTEYIQHTGSAVFA
                     CPSGVGP"
     gene            complement(1233..2135)
                     /gene="efeO"
                     /locus_tag="AC16CM_00840"
     CDS             complement(1233..2135)
                     /gene="efeO"
                     /locus_tag="AC16CM_00840"
                     /inference="COORDINATES: protein motif:HMM:NF041757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HIR3138402.1"
                     /translation="MKRVNNLARFVIVGALLALVATGCNVDREWVTSRDPSEAEAQEE
                     MQEQAAFFDIEGSPELDRAAREYQDYVDGQSELLVVRTEEFTSAVKAGDVAEGKELYA
                     SSRVPWERIEPIAEKFGTLDPEVDAREGDVPQRQWGGFHRIEQALWVENTTKGQGEYA
                     DELMSDVERLERKVGGVKLLVSDPVFGAVELLNEVSATKITGEEERYSHTDLYDIAAN
                     VEGAKVAFDFVKPVVKRENPELVAEIEARFESVEREIEPYRRGDGWVSYDQVDEDQRR
                     GLSQKIDALAEPLSRVGRVLSGGS"
     gene            complement(2146..2988)
                     /locus_tag="AC16CM_00845"
     CDS             complement(2146..2988)
                     /locus_tag="AC16CM_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR3138403.1"
                     /translation="MLAAFVVLFREGFEASLLVAIVLAYLAQIGQGKQRRQVWYGVGA
                     AIAVSLGLSGFLFATVGELEGPAEKIFEACALFLAVGFLTYMVLWMRREARALAGNIR
                     HEVDSAIGQGGGLALAFLVFIMVLREGVETGLFVFGIARASTPLQTAIGATAGILAAV
                     GLGYAVYALGTRINLGAFFKYTGAFLILVAAGLLAQGVVGLQEAGVVPAIFYPLWDVS
                     EMPVLGATSAVGQFLGILVGWDPRPDLLEFSVWLAYLLIVGYAFFKPQEPVAGRGTQT
                     ARPA"
     gene            complement(3451..3717)
                     /locus_tag="AC16CM_00850"
     CDS             complement(3451..3717)
                     /locus_tag="AC16CM_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HIR3138404.1"
                     /translation="MDEQRLDGNATAGILEEVFAFEITTARAACVQCGATGEVGAQMA
                     YVTEIGTVLRCSSCDSPLIRIVRQDENPRRYWLDLKGVDYLQFQ"
     gene            complement(3720..4466)
                     /locus_tag="AC16CM_00855"
     CDS             complement(3720..4466)
                     /locus_tag="AC16CM_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HIR3138405.1"
                     /translation="MVGTTRRLTWRVGEIAETRPRTPRTRSLFVDVPGWRGHRAGQHM
                     DIRLTAEDGYQAERSYSIASAPENDRVELVVERLDDGEVSPYLTDELRVGDGLELRGP
                     VGGWFAWDAEEGGPLLLVAGGSGIAPLMAMARHREAAGSDTPVRLLYSSRSYPEIIFR
                     GELETLAAGDGTLEVVHTLTRSRPEGWSGYDRRIDAEMLREVAYQPEERPLAFVCGPT
                     PLVETVATALVELGHDPARVKTERFGPTGG"
     gene            complement(4468..>5067)
                     /locus_tag="AC16CM_00860"
     CDS             complement(4468..>5067)
                     /locus_tag="AC16CM_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIR3138406.1"
                     /translation="ISRGFRGRRREPEGTSERVPPGQYLVDGFPVLSAGPTPRTPLLE
                     WSFTIEGEVDGPQTWSWDEFQALPSERLTRDIHCVTKWSKLDTTWEGVSVDTLLEGVE
                     TAAGYVLAYSDGGYTTNLPLEEVTGGKAWIAHTYEGEPLAPEHGGPARLLVPHLYFWK
                     SAKWVRGLRLMNGDEPGFWESLGYHNFGDPWREQRYWGD"
BASE COUNT          832 a         1747 c         1584 g          904 t
ORIGIN      
        1 ctggcccgac gccggacgga caggcaaaaa cggcgctgcc ggtgtgttgg atgtactccg
       61 tcaggcggtc gctctggccg aggcgccgct ggagcggaac gaattgctct ccggggtcgc
      121 gttggaaaga gatgaagaac aggcccgcgt cgagctgacc ggtcttggag tccatgccgt
      181 cggtaaaaga gtagccgcgg cgcaggatgc gctcgttcgg gttctggcgg gacaggcgca
      241 cgtgggagtc catcgggatc gccggtaggc cgtctgggcc cttcgccttg aggtctacgg
      301 ggtcgaactc gtcctgtttg cctatcgggg cgccgctggc cctgtggcgg cctatcgtct
      361 gttcctgttc aagcagcggc gaccggtccc agatctctat cagcatcctg atcctacgcg
      421 ccaccaggta ggttccaccc ttcatccagt ccggcccttc cctgacccag acgtagtcgt
      481 ccatcgcccc ttcgtcttcg gcgcggatgt tgttcgtccc gtctttgaag cccatcaggt
      541 tgcgcggggt cttctgggcg ctgctggtgc tcgaggtgcg cccgaagccc agctgggacc
      601 accggatcac ggccaggccg cggccgatcc tggccaggtt tctgacggcg tggaaggcga
      661 tctgcgggtc gtcggcgcag gcctggacgc agaggtcgcc gccggagagg cttgggtcga
      721 ggttgtcccc aggaaggtcg ggtatgggct ggagcgcccc tggcctgcgt gaggccagcc
      781 cgaaacggtc ctcgccgtcc ttctcgaaca gcgtcgggcc gaacccgaag gtgatcgtga
      841 ggtgcccggc cgtcaggtcc tgggcctccc cggtgtcatc gggcggcacg aaggcgtctt
      901 cgttctcgcc gccaaccagg tgtcccgcgc acatcgtcgc cgcggcctcg gaccacgccc
      961 gcatcaactc cctgacctcg cccgcgtcgg aggtggtcag gtcgaaggag gcgaagtgca
     1021 gccggtcctg ctggggggtg gcgatgccgg cctggtgttc gccgtagaac tcgacggtct
     1081 ggtcggcctg cgcgagctcc ctggccgaca gggcgccgcc cgtgccgagg gtcagccccg
     1141 ttccgaaggc acctgccagc gtcagggcgc cacggcggct tatcttgcgt ccgagcaggg
     1201 acccttctgt ctcctcgtgc ttaccgattt gtctacgaac ctccactcaa tacccggcca
     1261 accctggaaa ggggttcggc gagcgcgtcg atcttctggc tcagcccacg ccgctggtct
     1321 tcgtctacct ggtcgtagct gacccagcca tcgccgcgac ggtagggctc tatctcccgc
     1381 tccacgctct cgaatcgagc ctcgatctcg gccacgagct ccgggttctc gcgctttacg
     1441 acgggcttga cgaagtcgaa ggcgacctta gccccctcca cattcgccgc tatgtcgtag
     1501 aggtcagtgt gcgagtaacg ctcctcttcg cccgtgatct tggtcgccga tacctcgttc
     1561 aacaactcca cggccccgaa tacgggatcg gagaccagga gcttgacgcc gcccaccttg
     1621 cgctccagac gctcgacgtc ggacatcagc tcatcggcgt actcgccctg gcccttcgtg
     1681 gtgttctcca cccagagggc ctgctctatc ctgtggaaac cgccccattg cctctgggga
     1741 acgtcgccct ccctggcgtc cacctcgggg tcaagggttc cgaacttctc cgcgatcggc
     1801 tcgatcctct cccacggtac ccgcgaactc gcgtagagct ccttgccctc cgccacgtcc
     1861 ccggccttga cggcggacgt gaactcttcc gtgcggacca cgagcaactc gctctgcccg
     1921 tccacgtagt cctggtactc gcgggcggcc ctgtccagtt ccgggctccc ctcgatgtcg
     1981 aagaaggcgg cttgctcctg catttcctcc tgggcctcgg cttcggacgg gtccctggag
     2041 gtgacccact ctcgatccac gttgcatccc gtcgcgacga gcgccagcag ggctcctacg
     2101 atcacaaacc tcgccaggtt gttgactctc ttcaacgaaa tcctcctagg ctggccgtgc
     2161 ggtttgcgtg ccccgtccgg cgacgggttc ctgcggtttg aagaacgcgt aaccgacgat
     2221 aagcaggtac gccagccaca ccgagaactc caggagatcc ggcctcgggt cccagccgac
     2281 cagaatgccc aggaactgcc cgacagcgct cgtcgcgccg agaacgggca tctcgctcac
     2341 gtcccacagg ggatagaaga tggcgggaac gacgccagct tcctgcaggc cgaccacacc
     2401 ctgcgccagt agtcccgcag ccacgagtat gaggaaggcc ccggtgtact tgaagaaggc
     2461 accaaggttg atcctcgtcc caagcgcgta gaccgcgtac ccgagaccca ccgccgcgag
     2521 aatgcccgcc gtcgcgccga tggctgtctg aagcggcgtg gaggcgcgcg ctatgccgaa
     2581 gacgaaaagc cctgtctcga ccccctcacg caacaccatg atgaagacga ggaaggcgag
     2641 cgcgaggccg ccgccctgtc cgatcgcact gtccacctca tgccggatgt tccccgccag
     2701 ggcgcgggcc tcccggcgca tccagagcac catgtaggtc aggaaaccta ccgcgaggaa
     2761 cagcgcacag gcctcgaaga tcttctccgc cggaccttcc agctcgccaa ccgtggcgaa
     2821 cagaaagccc gaaagcccga gcgagacggc gatcgccgcc ccaaccccat accagacctg
     2881 ccggcgctgc ttgccctgtc ctatctgggc gaggtaagcc agcacgatag caaccagaag
     2941 gctcgcctcg aacccttccc tgaatagaac cacaaaggcc gctaacatgc gtgctccctc
     3001 tccagaacga cagaccaact cacgctaatg ataatcattc gcattagatc cgaagaatat
     3061 gcctgccttg aacaaaggtc aagccgctgc gtgaccaaag tcacctggag cacgctccgg
     3121 ctagcgccta cgctcgtcag cacgccggct ccgctccgct acgccggctt gtcagcacgc
     3181 ttcgaccctg cgggtctcgc ttgtcagcaa cgcttcggct tcgcctcgct ttcggctggt
     3241 cgtctcgatc cgcaaccgcg gcttgggcaa ccaacctttt ccgggcatcg aatctctaga
     3301 gcggcaggga gcaggccgca gagggatctg gctgaaagcg agccaggcgg agccgggcga
     3361 agcgttgctg acaaggcgcg tagcgccgtg ctgacaagcg aggccgcagg gccgagcgtg
     3421 ctgacaagcg taggcgctag ccgaagcgtg ctactgaaat tgcaggtagt caacgccttt
     3481 caggtcgagc cagtagcgtc tggggttctc gtcctggcgg acaatgcgga tcagggggct
     3541 atcgcatgag gagcagcgaa gaacggtgcc tatctcggtc acgtaggcca tctgggcgcc
     3601 gacctcgccc gtggcgccgc actgtacaca ggccgccctt gcggtggtga tctcgaaggc
     3661 gaagacctct tctaggatgc ctgctgtggc gttgccgtcg agcctctgtt catccatctc
     3721 tagcctcctg tgggtccgaa gcgttcggtt ttgacccggg cggggtcatg gcccagctct
     3781 acaagcgccg tcgccacggt ctcgaccagc ggcgtggggc cgcatacgaa ggcgagcggg
     3841 cgctcttcgg gctggtaggc cacttcccgg agcatctctg cgtcgatcct gcggtcgtag
     3901 ccggaccaac cttccggcct ggagcgggtg agggtgtgga ccacctccag cgtcccgtcg
     3961 ccggcggcga gggtctccag ctccccgcgg aagatgatct cggggtagga gcgagaggag
     4021 tagagcagcc tgacaggtgt gtcgctgccc gccgcctcac ggtgtctggc catcgccatg
     4081 agcggcgcga taccggaccc cccggcgacg agaagcagcg gcccgccctc ttccgcatcc
     4141 caggcgaacc agcccccgac ggggccgcgc aactcgagac cgtctccgac acgcaactcg
     4201 tccgtcaggt agggggagac ctcgccatcg tcgaggcgct cgactacgag ctcgacccgg
     4261 tcgttctctg gtgccgatgc tatggagtag ctgcgctctg cctggtagcc gtcctcggcg
     4321 gtgagcctga tgtccatgtg ctggccggcc ctgtggcccc gccaccctgg cacatccacg
     4381 aagaggctcc tggttcgcgg cgtcctgggc ctggtctcgg cgatctcgcc tacccgccag
     4441 gtgagcctgc gtgtggttcc caccacgcta gtcgccccag taacgctgtt cgcgccaggg
     4501 gtcgccgaag ttgtggtagc cgagagattc ccagaatccg ggctcatctc cgttcatgag
     4561 cctcaggccg cgcacccact tggcgctctt ccagaagtag aggtggggga cgagcagtcg
     4621 cgccgggccg ccgtgctccg gcgccagcgg ctcaccctcg taggtgtggg cgatccaggc
     4681 cttccccccc gtgacttcct ccagcgggag atttgtcgtg tagcccccgt cggaataggc
     4741 gaggacgtag ccggctgcgg tctctacccc ttccagcagg gtatctaccg acacgccttc
     4801 ccaggtggta tccagcttcg accacttggt gacgcaatgg atgtctcgcg taagcctctc
     4861 cgaaggcagc gcctggaact cgtcccacga ccatgtctgt ggcccgtcga cctcgccctc
     4921 gatcgtgaag ctccactcca agagcggcgt tcgtggcgtc gggcccgcgg agagaacggg
     4981 gaacccgtcg accaggtact gtcctggcgg cacgcgctcc gaggtaccct ccggctcgcg
     5041 cctacgaccg cggaaaccgc gggagat
//
LOCUS       DBNGVD010000033        10091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5208, whole genome shotgun sequence.
ACCESSION   DBNGVD010000033 DBNGVD010000000
VERSION     DBNGVD010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5208"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /gene="thrS"
                     /locus_tag="AC16CM_00865"
     CDS             <1..1476
                     /gene="thrS"
                     /locus_tag="AC16CM_00865"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3138407.1"
                     /translation="YQDNPYKTEIIEALEDGDITVYRQGDFFDLCRGPHVPSTGRLGA
                     FKLQNIAGAYWRGDEKNPMLTRVYGTAWPTEKELKAYLRRLEEARARDHRKIGKDLEL
                     FTFSPDTGAGIPLFLPRGEMMRYLMENYVREVQTAHGYEHVWTGHLVNEALYAKSGHL
                     EHYRESMFPPMVDGDTRYRLKPMNCPSHMTLFNSRAHSYRELPIRYAEFATLYRYEKS
                     GELNGLTRVRSLTQDDAHVFCTEDQIQQEFARALEIVREVLDTYGFADYRVQLSLRDQ
                     DSAKYVADDAKWSRAETELREALDTAGIAYHEETGEAAFYGPKADFMARDVLGREWQL
                     STIQVDFIQPGRLGCEYIGEDGKAHTPVLLHRAVTGSTERFMGVVIEHFAGAFPVWLA
                     PVQTVVIPVADRHVGYAREVQGKLAESGLMRVEVDDSANSMQKKIRENARQKIPYLLI
                     VGDREAEEGTVNVRRRGEKQQEEMHLEAFAQDILQEVRSRR"
     gene            1694..2248
                     /gene="infC"
                     /locus_tag="AC16CM_00870"
                     /pseudo
     CDS             1694..2248
                     /gene="infC"
                     /locus_tag="AC16CM_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            2354..2551
                     /gene="rpmI"
                     /locus_tag="AC16CM_00875"
     CDS             2354..2551
                     /gene="rpmI"
                     /locus_tag="AC16CM_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3138408.1"
                     /translation="MPKMKTHQGAAGRFVARKNGRLKRRRAGHNHFLEKKSPQQKRRL
                     NAPTDLTRSDEKKLKRMLGER"
     gene            2553..3110
                     /gene="rplT"
                     /locus_tag="AC16CM_00880"
     CDS             2553..3110
                     /gene="rplT"
                     /locus_tag="AC16CM_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3138409.1"
                     /translation="MARTARSVHARKKRRKVLDQAKGYRGTKHTSYKRAKEQVWKSGV
                     YAYEGRKQRKRDFRALWIQRINAGVREHGLSYSRFIHGLKLAEIDLDRKILADLAVTE
                     PEIFAAIVVQAQNALDGKPVERRVEVERAVAATQPETSEDVNATEAAERKAEELGVDI
                     TTVEGSGQGGRVVVGDVDDAAESDE"
     gene            3118..3804
                     /locus_tag="AC16CM_00885"
     CDS             3118..3804
                     /locus_tag="AC16CM_00885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3138410.1"
                     /translation="MPGTPAISPRRAAKLKAKKYRESERLFLAEGAQLLEEAGAPPVQ
                     VFDTEEQILRVSTLKTPAGPVGVFGFLDVPVPELLASVTRVMLLHGVQDPGNVGTAIR
                     SAHAFGAGIALSRGSADLYNPKTVRASMGSIFHAPVAREVESVAFLNAARVAGFVTVA
                     AMPGAGETPRSLPHSPLVICIGAEGAGLPEQVREACDTRITIPSLAPSLNASVAASIL
                     LYEAYSRVLP"
     gene            3820..4842
                     /gene="pheS"
                     /locus_tag="AC16CM_00890"
     CDS             3820..4842
                     /gene="pheS"
                     /locus_tag="AC16CM_00890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3138411.1"
                     /translation="MSERIEELRSEALSAVAAGDSTAVLEDVRVRYLGRSAELTEIKK
                     SIGSLSPEERKDVGRSSNLASREIEAALDSRLDEVSAREREERLREEAVDITLPGSPY
                     PRGHLHPSLRVIDEVVDFFVGLGYRVAEGPEIETDYYNFTALGIPPGHPARSMQDTFF
                     LDEGLSLRTHTSALQIRTMLAQDPPVYIVCPGRVYRRDSDPTHTPMFHQIEGLAVDRG
                     LTLGHLKGTLAAMARHVFGSGVKIRLRPSYFQYTEPSVELDVSCFVCGGTDPGCKVCK
                     GAGWLEMLGAGMVDPGVLEEVGYDPEEYTGFAFGMGPDRMAMVKYDVPDLRLFFEGDL
                     RFLRQF"
     gene            4862..7294
                     /gene="pheT"
                     /locus_tag="AC16CM_00895"
     CDS             4862..7294
                     /gene="pheT"
                     /locus_tag="AC16CM_00895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3138412.1"
                     /translation="MRVPLTWLHEYLDFDQTASELVDLFALHSQEIDGVYQFGVADGE
                     VVVGEVVEFGPHPNADRLFVAKVDLGGEEVQIVAGAPNPYPGARVPVVLPGSVLADGT
                     KLRKAKLRGLESYGMMMSERELGVSADHAGILLLDEAHSVGRPVADYFPVGETVLDID
                     VTPNRPDLWGMIGVARELAAILGVGYRIPGFVPQSGGRPSADFGLRVEDEDLCPRYDL
                     RRVSDLIPGRQAPVWMRRRVYAAGMRPINAVVDATNYVMLETGQPIHAFDASKVQDGI
                     VVRRARRGEKITLLDGSTRRLDEEMLVIADEERGLVIAGIMGAEDAEVGEETIDTLIE
                     VANFTGRNILRTSQKLGLRTDASGRFERGLDPNMVDFAMQRVTALLTHFPGGRVAEDD
                     LSHYPEPVDPWQVGMRLDRAELLLGIPVEPDGAVERLRALGCEVEREDGRLSAVVPTF
                     RRDLRREVDLIEEVGRLIGLDRVPETLPAVPQAGRLTETQQRDRNLRRLLAALGFAEA
                     ITYPFGPERWTKDLGLDDDTVVMQNPLSVEGSRLRTTVLSGLLDAAARNRSFGARGAS
                     LFEVGTVFSDDPLPGDVRDVALRFRMTGDPGGEDEELLMGVREENRVGVMLAGTVRPA
                     GWSVPEVRAGFFEAKGIVERLVPGARFVRAEKPFLHPGRSAIVLVGEKEAGWVGEVHP
                     EVAERFELAGWPVAALELHVALCQPDPEPRFEAFVNVPAVARDLAVLVEEGQPVGDIL
                     DAVYALSSSMLTEARVFDVYAGTQVPDGQKSVALAFTFQGEKTLTDAEVDDEVGRIVA
                     GLEEAFGARVRS"
     gene            complement(7284..7850)
                     /locus_tag="AC16CM_00900"
     CDS             complement(7284..7850)
                     /locus_tag="AC16CM_00900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138413.1"
                     /translation="MRLGARTRKLEGETVELRRHARSNYGLYATWYGDPEIWHLTSWA
                     PHPLSRSAVERLFEDRELSPVDDSFAIHVKDSEVPVGVISLMNISGANESAELSVIVG
                     HPDDRHRGYGTEAIELLLRYGFEELGLNRVGLSAFDFNGEAIAAYEKLGFAVEGRYRQ
                     AIKRDSGFHDAILMSIVRDEWRPNSASS"
     gene            8091..>10091
                     /locus_tag="AC16CM_00905"
     CDS             8091..>10091
                     /locus_tag="AC16CM_00905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3138414.1"
                     /translation="MPKPVGRSQRYMPGLDGLRAIAVLAVIAYHLDFGWAPGGLLGVG
                     VFFTLSGYLITDLLLGTRAATGSLKLGEFWIRRARRLLPALYLMIVAVVAWVTLFERT
                     FLSGLRGDVIAAFFYVSNWWNIAREASYFARFGPPPPLEHLWSLAVEEQFYLIWPWLV
                     WLVLFVTRKRPVTAGLALTGLALGLSAASVIAMWVLYQPGIDPTRVYEGTDTRAFGLL
                     IGAALAVVWPSRALSASLAHRSRVLLDGIGVVGLVIIALLIWRTSEYSPFMYHGGILL
                     LTLATTATVAALAHPATVLGTAVGWAPLRWIGVRSYGIYLWHWPIIVLTDPPLDQEPT
                     LTLQALQVAATIIVAAISWRFVEEPIRRGAIGNLWGKLRSGEWRRPASSRRQRRRRFA
                     GTAVALVLVGVLALDAAGLSGIVAPQTSESSKMTAAAQKEPSHPNVSSRNTVEVPGKA
                     GEEKGSGGDDGARESTNSADEEQASGSGDAASKNSTSCESVAYIGDSTSEGMVLPNYL
                     PNPKQRLDAQYARVGATKQFFEISGARSIVETLSTTQASGLELVKQLNAEGFEGCWVI
                     GLGTNDAANVYVGSAVALQPRVDQMMKLMGDQPVLWVTVRSLVTSGPYADENMQKWNQ
                     TLEANCDKYPNLRVFDWASLTRDDWFIEDGTHYTSEGYRHRAR"
BASE COUNT         1860 a         3121 c         3369 g         1741 t
ORIGIN      
        1 taccaggaca acccgtacaa gacggagatc atcgaggccc ttgaggacgg agatatcacg
       61 gtctacaggc agggcgactt tttcgacctg tgccgcggcc cgcacgtccc gagcaccggc
      121 cgcctcggcg ccttcaagct gcagaacata gcgggcgcct actggcgggg cgacgagaag
      181 aaccccatgc tcacacgcgt ctacggtacg gcctggccga cggagaaaga gctcaaggct
      241 tacctcaggc gtctcgagga ggcgagggcg cgggaccacc gcaagatcgg caaggacctg
      301 gagctcttca cgttctcgcc ggatacgggt gccggcatcc cgctcttcct gccgaggggc
      361 gagatgatgc gctacctcat ggaaaactat gtgcgcgagg ttcagacggc ccacggctac
      421 gagcacgtgt ggaccgggca tcttgtcaac gaggccctct acgccaaatc ggggcacctc
      481 gagcactaca gggaatcgat gttcccgccc atggtcgacg gcgacacccg ctatcggctc
      541 aagcccatga actgcccgag ccacatgacg ctcttcaact ccagggcgca ctcctacagg
      601 gagctcccaa tcaggtacgc cgagttcgcc accctctacc gttacgagaa gagcggggag
      661 ctcaacgggc tcaccagggt gcgctccctc acccaggacg acgcccatgt cttctgcacg
      721 gaggaccaga tccagcagga gttcgcccgg gccctggaga tcgtgcgcga ggtgctcgat
      781 acctacgggt tcgccgacta tcgggtccag ctctcgctgc gcgaccagga ctcggccaag
      841 tacgtggccg atgatgccaa gtggtcgcgg gccgagacgg agctccggga ggccctcgac
      901 accgcaggta tcgcctacca cgaggagacc ggcgaagccg ccttctacgg tcccaaggcc
      961 gacttcatgg ctagggatgt gctaggccgc gagtggcagc tctccacgat ccaggtcgac
     1021 ttcattcagc cgggccggct cgggtgcgag tacatcggcg aagacggcaa agcccacacc
     1081 cccgtactac ttcacagggc cgtaaccggc tccacggaga ggttcatggg cgtcgtgatc
     1141 gaacacttcg ccggcgcctt tcccgtatgg ctcgcccctg tccagaccgt ggttatcccc
     1201 gtcgccgaca ggcacgtggg ctacgctcgc gaggtgcagg ggaagctcgc cgagagcggc
     1261 ctgatgaggg tcgaagtcga cgactctgcc aacagcatgc agaagaagat ccgggagaac
     1321 gcccgccaga agatccccta cctgctgatc gtcggcgaca gggaggccga agaaggcacc
     1381 gtcaacgtcc gccgccgcgg cgagaaacag caagaggaga tgcatctaga ggctttcgcc
     1441 caggacatct tgcaagaggt gcgttctcgc aggtagtgcc gtcagctatg gaccttcagc
     1501 tatcgcttct tgttcgttgc ctgaccgctg gaggctatct agacacgaag cgacggcccg
     1561 cggtataatc aagggtgtag ttggggtccg cgtctgttcg acgcacaagc aaacctagcg
     1621 tccgtggagg gcgaacggcc ccataaatcc attttgagag gagtggtgcg cagtagcaac
     1681 tgaagaagag ccgcgaataa acggtcagat ccgggcgcgt gaggtgcgtc tcatctcggc
     1741 cgacggcgag cagctaggca tcaagcacat ccgcgaggcc atggattacg gcgagagggc
     1801 cgacctggat ctggtggagg tggcacccaa cgccaacccg cccgtggtcc gtatgatgga
     1861 ctacggcaaa ttcaagtacc agaaagagca ggcccggaag gccgcccgca aaaagcaggt
     1921 caacgtaaac gtgcgggaga ttaagctcag gcccaagatc ggcgaccacg acttcaacac
     1981 caagcgtggc cacgttgagc gtttcctgcg cggtggcgac aaggtgaagg tgaccatcat
     2041 gttccgtgga cgcgaggtcc agcacccgga cctgggggag aggctcctca ggcgtctcgc
     2101 gaccgacctc gacgaccttg gtcgcatcga gagccagccc aacctggacg gtcgtaacat
     2161 ggtcatggtc atggccccca agaaagaggc ccagcaggcc gagaacgccg agccgggcga
     2221 acgcagggag cgggccgctc gcagataagc aacagtttga tacactagcc tgacgcattg
     2281 aggaggcgaa ccggctctct cccgtgccgc gagccctttt gcaggtagta cagaccagaa
     2341 acgtggagga aaaatgccga agatgaagac ccaccagggc gctgccgggc gcttcgtggc
     2401 gcgcaagaac ggcaggctca agcgccgccg cgccggccac aatcatttcc tggagaagaa
     2461 gagcccgcag cagaagcgcc ggctcaacgc gccgaccgac ctgacccgct cggacgagaa
     2521 gaagctcaag cgtatgctgg gggagcgcta gcatggcgcg tacggcacgc agcgtccacg
     2581 cccgcaagaa gcgccggaag gtgctggatc aggcgaaggg gtatcgcggt accaagcata
     2641 cctcctacaa gcgggccaaa gagcaggtct ggaagagcgg ggtctacgcc tacgagggac
     2701 gcaagcagcg caaaagggat ttcagggcgc tctggatcca gcgcatcaac gccggggtca
     2761 gggagcacgg cctctcttac tcgaggttca tccacgggct gaagctggcc gagatcgacc
     2821 tggaccgcaa gatcctggcc gaccttgcgg taacggagcc tgagatcttc gccgcaatcg
     2881 tggtccaggc ccagaacgct cttgacggta agcccgtcga gcgtcgtgtc gaggtagagc
     2941 gggccgtcgc ggcgactcag ccagagacct ccgaagacgt gaacgctacc gaggcggccg
     3001 agcgcaaggc cgaggagctc ggggtggaca tcacgaccgt ggaaggatcg ggccagggcg
     3061 ggcgcgtggt cgtgggggac gtggacgacg ccgcagaaag cgacgagtaa cggcggattg
     3121 ccaggcaccc cggcgatctc ccccaggcgg gctgcgaagc tcaaggccaa gaagtaccgc
     3181 gagtccgaac gactgttcct ggcagaaggc gcgcagctcc tcgaggaggc tggcgcgccc
     3241 ccggtccagg tcttcgacac ggaggagcag atcctccgcg tctctacgct aaagaccccg
     3301 gccggtccgg tcggggtctt cggctttcta gacgttcccg ttccggagct acttgccagc
     3361 gtaacccggg ttatgttgct tcatggggtg caggacccgg ggaacgtggg gacggccatc
     3421 cgctctgcgc atgccttcgg ggccggtatc gcgctctcca ggggctctgc cgacctgtac
     3481 aaccccaaga ccgtcagggc ctcgatgggc tccatatttc acgctcccgt cgcgcgggag
     3541 gtcgagtcgg tagcgtttct gaacgcggcg agagtggccg gtttcgtcac ggtggccgcg
     3601 atgcccgggg caggggagac gcccagaagc ctgccccata gcccgctcgt tatctgtata
     3661 ggcgccgaag gggcaggatt gcccgaacag gttcgcgagg cttgcgatac acggatcacg
     3721 ataccctccc tcgcaccgtc gttgaacgcc tcggtggcgg cctccattct gctctacgag
     3781 gcgtattcgc gtgtgctacc ataatcgccc attatgacgg tgagcgagcg catcgaagag
     3841 ctgagatcgg aggccctctc cgccgtcgcc gctggcgatt caactgcggt cctggaagac
     3901 gtgcgggtgc gatatctggg acgttcagcg gagcttacgg agatcaagaa gagcataggc
     3961 agcctctccc ctgaggagcg caaagacgtg ggcaggtcct ccaacctcgc ctcgcgtgag
     4021 atcgaagccg ccctcgactc gcggcttgac gaggtttcgg cccgcgagcg ggaagagcgg
     4081 ttgcgcgagg aggcggtgga catcacgcta ccgggctcac cataccctag aggccacctg
     4141 catccgtcgc tccgggtcat cgacgaggtg gtcgacttct tcgtggggct tggctaccgc
     4201 gttgccgaag ggcccgagat cgagacggac tactacaact tcacggcgct tgggatccca
     4261 ccgggacatc ctgcgcgcag catgcaggac acgttcttcc tggacgaagg gctctcgcta
     4321 aggacccaca cctcggcgtt gcagatcagg acgatgctcg cgcaggaccc ccccgtgtac
     4381 atcgtatgcc cggggcgggt ctaccgccgg gattccgacc ctacgcacac gcccatgttc
     4441 caccagatcg agggcctcgc cgtggaccgg gggctgacgc tcgggcacct gaaggggacg
     4501 ctcgccgcca tggcccgcca tgtctttggt tctggcgtga agatccggct ccgccccagt
     4561 tacttccagt acaccgagcc aagcgtggag ctggatgtta gctgcttcgt gtgcggcgga
     4621 accgaccccg ggtgcaaggt ctgcaagggc gctggctggc tcgagatgct cggcgccggg
     4681 atggtagatc ctggggtcct cgaggaggtc ggctacgacc ccgaggaata tactggcttc
     4741 gccttcggga tgggtccgga caggatggcc atggtcaagt acgacgtgcc agatctgcgg
     4801 ctgttcttcg agggcgacct ccgcttcctg cgtcaattct gatccttaga agggaaacac
     4861 cttgcgcgtt ccgttgacct ggctccacga gtacctcgac ttcgaccaga ccgcctccga
     4921 actggtcgac ctgttcgcct tgcacagcca ggagatagac ggtgtctacc agttcggggt
     4981 tgccgacggc gaggtcgtcg tcggggaggt tgtggagttc ggtccacatc ccaacgcgga
     5041 ccggctcttc gtggcgaagg tagatctggg tggtgaagag gtgcagatcg tggccggcgc
     5101 cccgaatccc tatccaggcg cccgcgtgcc cgtcgtgctg cccggaagcg tcctggctga
     5161 cgggacgaag ttgagaaaag ccaagctcag gggcctcgag tcttacggga tgatgatgag
     5221 cgaacgcgag cttggcgtct ccgcggacca cgccggcatc ctcctgctcg atgaggcgca
     5281 ctcggttggg cgccccgtcg ctgactactt ccccgtcggg gagaccgtgc tcgacatcga
     5341 cgtcaccccc aacaggccgg acctgtgggg catgatcggg gtcgcaaggg agctcgcggc
     5401 tatcctcggg gtcggctacc gtataccggg gttcgttccg cagtcgggcg gcagaccgtc
     5461 ggccgacttc gggctgaggg tggaagatga agacctctgc ccgaggtacg acctgcgtcg
     5521 tgtctcggat ctcatccccg gaaggcaggc gccggtgtgg atgcgacgtc gcgtgtacgc
     5581 cgccggcatg cggcccatca acgctgtagt cgatgccacc aactacgtca tgctggagac
     5641 cggccagccc atacacgcct tcgacgcgtc gaaggtgcag gatggcatag tcgtgaggcg
     5701 ggcaaggagg ggagagaaga tcacgctcct cgacggttcc acccgccggc tggacgaaga
     5761 aatgctggtg atcgccgacg aggagcgggg cctcgtgatc gccgggatta tgggtgctga
     5821 ggacgccgag gttggagagg agaccataga caccctcatc gaggtcgcga acttcacggg
     5881 acgaaacatc ctcaggacct cgcagaagct cgggctgcgc accgacgcct cgggccgctt
     5941 cgagcggggc ctcgatccga acatggttga ctttgccatg cagcgggtga ccgcccttct
     6001 tacgcacttc ccgggcggac gcgtcgccga agacgacctc agccactacc cagagcctgt
     6061 cgacccctgg caggtaggga tgcgcctgga tcgggcggag ctcctgcttg gcattcccgt
     6121 cgagccggac ggtgcggtcg aacggctccg ggcgctcggg tgtgaggtcg agcgggaaga
     6181 tggccggttg tctgctgtgg tgccgacctt caggcgagac cttcggcggg aggtggacct
     6241 gatcgaggaa gtcggacgcc tgatcgggct ggacagggta cccgagacgt taccggccgt
     6301 tcctcaggcc ggcaggctaa cggaaaccca gcaacgggat cgtaacctgc ggcgtctgct
     6361 cgcagccctc gggttcgccg aggcaattac ctacccgttt ggtcctgagc gctggacgaa
     6421 agaccttggc ctcgacgacg atacagtcgt tatgcagaac ccgctcagcg tcgagggcag
     6481 ccgactcaga acgaccgtcc tctccgggct gctcgacgct gccgcccgca accgatcctt
     6541 cggtgccagg ggcgcctcgc tgttcgaggt gggcactgta ttctccgacg atccgctgcc
     6601 gggcgacgtg agggatgttg ccctgcggtt ccgcatgacc ggcgaccctg gcggcgagga
     6661 cgaagaactg ctcatgggcg tacgtgagga gaacagggtt ggggtcatgc tagccggaac
     6721 ggtgaggccg gctggttgga gtgttcccga ggtccgggct ggtttcttcg aggcgaaggg
     6781 aattgtcgaa cggctcgtgc ccggggccag gttcgttcgt gccgaaaagc cgttccttca
     6841 cccaggtcgc tccgccatcg tgctcgtcgg cgagaaggag gccggatggg tgggggaggt
     6901 gcaccccgag gtcgcggaga ggttcgaact ggccggttgg cccgtggcgg ccctggaact
     6961 ccacgtggcc ctctgccagc ccgatccgga gccccggttc gaggcattcg tcaacgtgcc
     7021 cgccgtcgcc agggatctcg ccgtcctggt tgaggagggg caacccgtcg gggatattct
     7081 ggacgccgtc tatgctctct ccagctcgat gctcaccgag gcacgcgtct tcgacgtgta
     7141 cgcggggacc caggtgcctg acggacagaa aagcgtcgcg ctcgccttca cgtttcaggg
     7201 tgagaagacc ctcacggacg ccgaggtcga cgacgaggtc gggcgcatcg tggccgggct
     7261 cgaggaagcg ttcggggcgc gcgtcaggag ctagcggagt tcggtcgcca ctcgtctctc
     7321 acgatgctca tcaagatggc gtcgtggaag ccggagtccc gctttatggc ctgtcggtag
     7381 cgtccctcta ccgcgaaacc cagcttctcg taggctgcga tggcctcacc gttgaagtcg
     7441 aaggcgctca ggccaacacg attcaggccg agctcctcga agccgtagcg caagaggagt
     7501 tctatggcct cggtgccgta gccccggtgg cgatcgtctg gatggcccac gataaccgag
     7561 agctccgccg attcgttggc gccgctaatg ttcatgaggc tgatcacgcc cacgggcact
     7621 tctgagtcct tgacgtggat tgcgaaagag tcatccaccg gagagagctc cctgtcttcg
     7681 aaaagcctct cgacggccga acggctcagg gggtggggcg cccaactggt gaggtgccag
     7741 atctcggggt ccccgtacca ggtggcgtag agcccgtaat tcgagcgggc gtggcggcgc
     7801 aactcgaccg tctcgccttc cagctttctg gttctcgcgc caagccgcac ggcagagaat
     7861 ataccgggtt cgattacgat acgccacgtt gcgtttcggt acctccgaga cacactctct
     7921 agtgccggtt gcaactcccc tgggtttcgt ggttagaata ggggccgaaa atcgggagtc
     7981 ctgacacaag gaggctgcgg gagtgacgta ctcgtccggc aaaaacctgt tcatagaatt
     8041 gccgggctgg cctggaacgg ggtccgcttc cgccagcggg gacgtccagc gtgcctaagc
     8101 cagtaggtcg ctcccagcgt tacatgcccg ggctcgacgg gctgcgggcc atcgccgttc
     8161 ttgccgtaat cgcctaccac ctggacttcg gttgggcgcc aggaggtttg ctcggggttg
     8221 gcgtcttctt caccctgagc gggtacctga tcacggatct tcttctcggg acgagggcag
     8281 ccacgggtag cctcaagctg ggtgagttct ggatcaggcg cgccaggagg ctcctgcctg
     8341 ccctctacct gatgatcgtc gcggtggtgg cgtgggtcac cctgttcgag aggacgttcc
     8401 tctccggcct gaggggcgac gtgatcgccg cattcttcta cgttagcaac tggtggaata
     8461 tcgcccggga ggcctcttat ttcgccaggt tcgggcctcc tccgccgctg gagcatctgt
     8521 ggtcgctggc ggtggaggag cagttctacc tcatctggcc gtggctggtg tggctggtcc
     8581 tgttcgttac ccgcaagcgc cccgtcaccg cgggcctggc gctgaccggc ctggcgctgg
     8641 gtctctcggc tgcctcggtt atcgccatgt gggtgctcta ccagcccggc atcgatccga
     8701 cgcgggtcta cgaggggacc gacacccgcg ccttcggcct gctcatcggc gcggcactgg
     8761 ccgtggtgtg gccaagccgg gccctgagcg caagcctcgc gcacaggtca cgcgtcctgc
     8821 tggacggcat cggcgtcgtg gggctcgtga tcatagcgct cttgatctgg cgcaccagtg
     8881 agtactcgcc gtttatgtac cacggcggta tcttgctgct gaccctcgcg acgacagcga
     8941 cggtggccgc gctggcacac ccggctaccg tacttggcac cgccgtaggc tgggcgccgc
     9001 tacgttggat aggtgttcgt tcatatggaa tttacctctg gcattggccg atcatcgtgc
     9061 tgaccgaccc acccctggat caggagccga ccctcacgct gcaggcgctg caggtggcgg
     9121 ctacgatcat cgtggccgcg atctcctggc ggttcgtgga ggagccgatt cgccgcgggg
     9181 ccatcgggaa cctgtgggga aagctgagat ccggggagtg gcgacgtccg gcatcgtccc
     9241 gccggcagcg ccggcgccgg ttcgccggga ccgccgtggc gctcgtgctg gttggcgtgt
     9301 tggccctcga tgccgccggt ctgtcaggca tcgtagctcc tcagacttcg gagagcagca
     9361 agatgacggc ggcagcccag aaagagccgt cacacccgaa cgtgtcctct cgcaacacgg
     9421 tagaggtccc gggcaaggcg ggggaagaga agggttctgg gggggatgat ggcgcacggg
     9481 agtcgacgaa ctccgcagac gaggaacaag cgtccgggtc tggagatgcg gcgtcgaaga
     9541 acagcacttc ttgtgagtcg gtagcgtata ttggcgattc gacctcggag ggaatggtgc
     9601 tgcccaacta cctgcccaac ccgaaacagc gccttgacgc acagtacgcg cgggtcggcg
     9661 ccacaaaaca gttcttcgag atttccggcg cgcgctccat cgtggagacg ttgagcacga
     9721 cgcaggcctc cggcctcgaa ctcgtgaagc aactgaacgc ggaaggtttc gagggctgct
     9781 gggtgatcgg gctcggcacc aacgatgccg ccaacgtcta cgtgggttcg gcggtggccc
     9841 tgcaaccacg cgtcgaccag atgatgaagt tgatgggtga ccagccggtg ctgtgggtca
     9901 cggtaaggtc gctggtgacc agcggaccct acgcggatga gaacatgcag aagtggaacc
     9961 agaccctgga agccaactgc gacaagtacc cgaatttgcg cgtcttcgac tgggcatcac
    10021 taaccaggga cgactggttt atcgaggatg gaacccacta cacctcggag ggctacagac
    10081 accgtgcccg c
//
LOCUS       DBNGVD010000034         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_187479, whole genome shotgun sequence.
ACCESSION   DBNGVD010000034 DBNGVD010000000
VERSION     DBNGVD010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_187479"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16CM_00910"
     CDS             complement(<1..329)
                     /locus_tag="AC16CM_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138415.1"
                     /translation="MERTVSVSWALFALLCVGLVLLMAPAWAQEGGIPPGTAARGVDT
                     ENNNRPPDVVVVAASGCTVAAGASITIEDGDGTRAIFTDGDRGITILSQDGHPRIEGP
                     PGDFVGD"
     gene            complement(436..1224)
                     /gene="attM"
                     /locus_tag="AC16CM_00915"
     CDS             complement(436..1224)
                     /gene="attM"
                     /locus_tag="AC16CM_00915"
                     /EC_number="3.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102007 - acyl-L-homoserine-lactone
                     lactonohydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AttM family quorum-quenching N-acyl homoserine
                     lactonase"
                     /protein_id="HIR3138416.1"
                     /translation="MDELRLYFFECGSLKTQVQFIKMNQGLGDPYEIPVPFFLITHPR
                     GNVLYDGGNALEVARDARGHWADVVDAYDPVMDEDDFVVSQLERMDVDPASVRYVIQS
                     HLHLDHSGAIGHFPNAEYVVQRRELEYAFTPDWFQKPAYIRKDFDRDVRWLFLDGEHD
                     DGYDVFGDGTIRTLFTPGHAPGHTSVIVDLPGEGKMMLTADACYTMDHYNETALPGLI
                     HSAADVADSTRKVRRIVEGHGATLVTGHDPEEWPRFKKAPDYYE"
     gene            complement(1399..2667)
                     /locus_tag="AC16CM_00920"
     CDS             complement(1399..2667)
                     /locus_tag="AC16CM_00920"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HIR3138417.1"
                     /translation="MGNETIFTMEATPLKYGPGASEEAGWELKRMGVGRVMLISDPGV
                     VEAGITGRIVELIEAEGIEVEVWDRSRVEPTDDSFGAAADFAAEGDFDGFVAVGGGSS
                     IDTAKVSDLISTHGGEIMDYVNAPVGGGKNPPGPLKPLLAIPTTAGTGAEATTVAILD
                     IPEQRVKSGISHQYLRPDRGVVDPLLTTSLPPEVTSSCGLDVVCHAAESFLSRPYDAR
                     PKPESPDERPPYQGANPIADMWSAKALEYGGKYLRRAVKNGEDVEARGAMMLGASLAG
                     VGFGSAGVHIPHACAYPIAGLKHEWQPPGYPEDHPFVPHGWSVIVTAPATFRFTFEAM
                     PERHKQVAELLSGEQIDRPDENTLPEILIQLMQDVGAPRGIRELGYTEDDIDDLVDGA
                     TKQQRLLVGSPRETSEEDLANILRESMENW"
     regulatory      2903..2986
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT          505 a          954 c          959 g          612 t
ORIGIN      
        1 tggtcgccga caaagtcgcc gggaggaccc tctattctcg ggtggccgtc ctgggatagg
       61 atcgtgatcc ccctgtcccc gtcggtgaag atggcccggg tcccgtcgcc gtcctcgatg
      121 gttatggacg ccccggccgc aaccgtgcaa ccgctggccg cgaccacgac cacgtccggc
      181 ggtctgttgt tgttttcggt atccaccccc ctggcggctg tgcctggcgg tatccctccc
      241 tcctgggccc aggccggcgc catcaggaga accaggccga cgcacagcag cgcgaagagc
      301 gcccaagaca cggaaacggt tcgctccatc ctcagcctcc ttcgccacgc gaacgatgaa
      361 aagtattgtg ccgtatggca cgcgcggtgc gcaagcgtgt cgtcagaccg ggtgcgcgcc
      421 ggaggcgccg tcccgtcact cgtagtagtc tggggccttc ttgaatctgg gccactcttc
      481 ggggtcgtgg cccgtgacca gggtggcgcc atggccttcg acgatgcgac gcaccttgcg
      541 ggtagagtct gccacgtccg ccgcggagtg gatcaggccc ggcagcgcgg tctcgttgta
      601 gtggtccatg gtgtagcagg catccgcggt gagcatcatc ttcccctcac caggcaggtc
      661 cacgatcacc gaggtgtggc ccggcgcgtg gcccggcgtg aagagggtcc ttatggtgcc
      721 gtcgccgaac acgtcatagc cgtcgtcgtg ctcgccgtcc aggaacagcc atctgacgtc
      781 cctgtcaaaa tcctttcgga tgtaggcagg cttctggaac caatcggggg tgaaggcgta
      841 ctcgagctcc cggcgctgga ctacgtattc ggcgttcggg aagtgaccta tggccccgga
      901 gtggtccagg tgcaagtgcg actgtatgac gtagcgtacg gaggccggat ccacatccat
      961 ccgctcgagc tggctaacga cgaaatcgtc ctcgtccatc acgggatcgt aggcgtcgac
     1021 gacgtccgcc cagtgcccgc gggcgtcccg cgcgacttcc agggcgttgc ccccatcgta
     1081 gagcacgttg ccccttggat gggttatcag gaaaaaggga accggtatct cgtaagggtc
     1141 accaagcccc tggttcatct tgatgaactg cacctgggtc ttgaggctgc cacattcgaa
     1201 aaagtaaaga cgcaactcgt ccatttgttc tcccttcggt cgaacgctat cagctttcag
     1261 caattagctt tcagcttttc tcgctcgacg tgtgccctga ctccaacgct ggtcaggtgg
     1321 gttttgccgc gttgaccctg taacgcctcg cctgccattg actggctgaa ggctgatagc
     1381 tgaccgctga aggctccatc accagttctc catcgactcg cgcaggatgt tcgcgaggtc
     1441 ttcttccgag gtctctctcg gggagccgac cagcaaacgt tgttgttttg tggcaccgtc
     1501 caccaggtcg tctatgtcgt cttctgtgta gccaagctcg cggatgcctc tgggggcacc
     1561 gacgtcttgc atgagctgta taaggatctc gggtagtgtg ttctcgtccg gccggtctat
     1621 ttgctcgccg gagagcagct cggccacctg tttgtggcgc tccggcatgg cttcgaaggt
     1681 gaaacggaag gtggcggggg cggtgacgat tacggaccag ccgtgcggga cgaaggggtg
     1741 gtcttcaggg tagccgggag gttgccactc gtgtttgagg ccggctatgg gataggcgca
     1801 ggcgtgggga atgtgaaccc ccgcagagcc gaagcccacc ccggcgaggg aggctcctag
     1861 catcatggca ccgcgggcct ctacgtcctc gccgttcttt acggcgcgcc ttaggtactt
     1921 gccgccgtac tcgagcgcct tggcgctcca catgtccgct atggggttgg ccccctggta
     1981 tggcggtctc tcgtccgggc tctccggctt cggtcgggcg tcgtagggcc tggagaggaa
     2041 cgattcggcg gcgtggcaga ccacatcgag gccgcaggac gaggttacct caggcggcag
     2101 gctcgtggtg agcagggggt ccacgacgcc gcggtcgggg cgaagatact gatgcgagat
     2161 gccgctcttt acccgttgtt cagggatatc gagtatggcc acggtcgtag cctcggcgcc
     2221 cgtgcccgcg gtcgtcggga tggccagcag cggcttcaag ggtcccggcg ggttcttgcc
     2281 cccgccaacc ggggcgttca cgtagtccat gatctcgccg ccgtgggtgg agatcaggtc
     2341 cgagactttg gccgtgtcga tgctcgaccc gccgccgacg gcgacgaagc catcgaaatc
     2401 cccctcagcg gcgaaatccg ccgcagcccc gaacgagtcg tccgttggct caacgcgcga
     2461 gcgatcccac acctccacct ctatgccctc ggcctcgatg agctcgacga ttcgtcccgt
     2521 tatgcccgcc tcgaccacgc cggggtcgga gatgagcatc acgcgcccga cgcccatgcg
     2581 cttgagctcc cagccagcct cctccgaggc gccgggcccg tacttgagag gcgtcgcttc
     2641 catcgtgaag atagtctcgt tgcccatgct tgtctccctt cccccgcgtc aggttgtatg
     2701 tctatacccg ctccacccga cggatatacg ttcgggtctc gtatccacca gcagagcgat
     2761 cccgaagcct gcgacggccg actagtccat ttgtcgtatg ccgatcggca tacggcaaga
     2821 gaccgaaata tgccgatcgg cagatgtgcc cactatatca agactatatc atttttgtta
     2881 caaggtcgga ggttcgccgg ccacatacgg tagaggcaaa gaaacccgtc cgtactctgg
     2941 gcgcccggag cgggtggagc cagtggcgca accggctacc tcggaggcga ggtggccggt
     3001 tttgtttgca atggcctcaa gatagaaagg
//
LOCUS       DBNGVD010000035         4220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1827383, whole genome shotgun sequence.
ACCESSION   DBNGVD010000035 DBNGVD010000000
VERSION     DBNGVD010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1827383"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..536)
                     /locus_tag="AC16CM_00925"
     CDS             complement(231..536)
                     /locus_tag="AC16CM_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138418.1"
                     /translation="MTENQEERHVEYGEEERVPEHSGTNHSMAIGFYIAAIVLIYGVT
                     IALAGIFSSGQQINANIGFNVNLWWGLVMVVVGCVILGMTFASPRRRAARREQQQQG"
     gene            complement(546..2174)
                     /locus_tag="AC16CM_00930"
     CDS             complement(546..2174)
                     /locus_tag="AC16CM_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3138419.1"
                     /translation="MVKLLNLAFLQEPLIEVNESWLDYFIIAVYFVFVLGIGAVLRTK
                     MQTSEDYFLSGRSLPSWITGLAFLGANLGALEIMGMGAGAATYGIMQSHYYWIGAIPA
                     MVFVALFMIPFYYASRVHSVPGFLKLRYNEATRGFNAILFAIFMLLLSGINMYAMALV
                     FQLLLGWSLTASIFLSAAIVLGYVVLGGLSSSIYNEVLQFFLITLGLVPLVIFGLIDV
                     GGISGLKEAIAKPEFFHAWSNTGSTDNPFAVQWFYIVFGLGLVQSFGYWCTDFLVVQR
                     ALAAEDLAAAQRTPLIAAFPKLLYGVLAIFPGLIVLAVLPRVATEDPNNAVPYAMVHY
                     FPSGMLGFGLTALLAAFMSGMAGNVTAFNTVWTYDIYKPYINRDASDRHYLNMGRIAT
                     VVGVLLSIVTAYIALNFTTIINYVQVLHGMFLAPLFGTFLLGMFWKRTTGWGGFTGLV
                     AGTGYGLITYLITLDEGLGASMARAFFAWVVTLAVTLAVSLVTQPKPDDELRGLVWGL
                     TEQKEAREEVWYKKPWVLAVTVLAITVALNIYFW"
     gene            complement(2617..3492)
                     /locus_tag="AC16CM_00935"
     CDS             complement(2617..3492)
                     /locus_tag="AC16CM_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138420.1"
                     /translation="MEYRRLGRLGRENSVLTFGGAALSETNEEDSDRAIQQALDAGVN
                     HFDTAADYGDSELEYGRWMGEIRDRIFLSTKTGLREKDAARRQIEASLERLRVDNVDL
                     LQLHAVGDLEDLDRATGSGGSLEAAIEAKEEGLVGGIGITGHGNGAPSTHLEALRRFP
                     FDTVLTPWNFILSTDEGYRADYNALVDEVKRQDAGLLIIKTISRRNWPEGDPTGDQRY
                     ATWYEPFDQQEYIDAAVSWILSQEEVTGLAMAGDVGLVPMILEAEKNRLSREEAEEVL
                     ARAPDYSSPFISMPF"
     gene            complement(3507..>4220)
                     /locus_tag="AC16CM_00940"
     CDS             complement(3507..>4220)
                     /locus_tag="AC16CM_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HIR3138421.1"
                     /translation="HVWWHERQVLLRALFPLRVRSHEATFETMHGVVRRPTHRNTSWD
                     EARFEVCAHRFADLSEPGYGVALLNDGKYGHSARDNVLGISLLRAPLYPDPLADQGEH
                     RFTYSLFPHPGDWTEAGVASEAHRLNSPLIVTEGRTDPAAYGLLEAEGLELALGALKP
                     AEDGRGGILRLYEPNGARGECVLRFAEAPGSVHRTSLLEEDEGAVEVHDGAVRLAVRP
                     FEVVTLRIEWEKDTGSRGS"
BASE COUNT          805 a         1321 c         1363 g          731 t
ORIGIN      
        1 tcatcgaaca acaagagata gtgtgaagcg ttcccatccc tggtgcaagg cgccggaacg
       61 ctgcgggagg ggttcgacgg ggtagaccta cccaccgtgg aacgaacttg cggccctcgg
      121 ggcagcgtcc gggccagatc agccggtcag aacccctgag gtcatcggag cgtaacccgt
      181 ctctcgcaga ccggcgccta ccccgacgag tagacgccgg ccccgcgaac ctagccctgt
      241 tgctgttgct cgcgtctggc ggcccggcgc cggggggatg cgaaggtcat cccgaggata
      301 acgcagccca cgacgaccat taccaggccc caccacaggt tgacgttgaa ccctatgttg
      361 gcgttaatct gttgtccgga agagaagatc ccggccaggg ctatcgtgac gccgtagatc
      421 aggacgatag ccgcgatata gaagccgatg gccatggagt ggttggtacc ggagtgctcg
      481 ggcaccctct cttcttcccc gtactctacg tgacgttctt cctggttttc cgtcatggtt
      541 tcctcctacc agaagtagat gttcagggcc acggtaatgg cgagcacggt gaccgcgagc
      601 acccagggtt tcttgtacca gacctcctcc ctggcctcct tctgctcggt caggccccac
      661 acgaggccgc gcagctcatc gtcgggtttg ggttgcgtga caagggagac ggcgagggtc
      721 acggcgaggg tcacgaccca cgcgaaaaac gctcgcgcca tggaggcccc aaggccttcg
      781 tcaagcgtaa tgaggtaagt gatgagaccg tagcccgtac cggccacgag gccggtgaac
      841 ccgccccagc ccgtagtgcg cttccagaac atcccgagca ggaacgtgcc gaatagcggt
      901 gccaggaaca taccgtgcag cacctgaacg tagttgatga tggtggtgaa gttgagggcg
      961 atgtaggcgg ttacgatgct aaggagcacc ccgacgacgg tggcgatgcg gcccatgttg
     1021 aggtagtggc ggtcggaggc atcccggttg atgtacggtt tgtagatatc gtaggtccat
     1081 acggtgttga aggccgtcac gttgccggcc atcccgctca tgaaggccgc gagcaacgcc
     1141 gtcaggccga acccgagcat cccggagggg aagtagtgga ccatcgcgta ggggacggcg
     1201 ttattggggt cctctgtggc gacccgcggt agcacggcga gcacgatcag gcccgggaag
     1261 atcgcgagca ccccgtacag gagcttcggg aacgccgcga tgaggggcgt cctctgggcc
     1321 gccgccagat cctcggccgc gagggccctc tggaccacga ggaagtccgt gcaccagtag
     1381 ccgaaggatt gcaccaggcc gagaccgaac acgatgtaga accactgcac ggcaaacggg
     1441 ttgtccgtcg agcccgtgtt ggaccaggcg tggaagaact cgggcttggc tatagcctcc
     1501 ttgaggcccg atatgccccc gacgtctata aggccgaaga tcacgagcgg caccagcccg
     1561 agcgtgatca ggaagaactg caagacctcg ttgtagatgg aggacgagag gccaccaagc
     1621 accacgtacc cgagcacgat ggccgcggag aggaagatgc tcgccgtgag cgaccatccg
     1681 agtagcagct ggaacacgag cgccatcgcg tacatgttta tgcccgagag cagaagcatg
     1741 aagatggcga acaggatggc gttgaacccg cgcgtagcct cgttgtagcg gagcttgagg
     1801 aagccgggta cagagtggac cctgctggcg tagtagaaag gaatcatgaa cagggccacg
     1861 aagaccatcg ccgggatcgc gccgatccag tagtagtggg actgcatgat cccgtaggtc
     1921 gccgccccgg cgcccatgcc catgatctcc agggccccga ggttggcccc gaggaaggct
     1981 aggcccgtga tccaactcgg caacgacctg cccgagagaa agtagtcctc ggaggtctgc
     2041 atcttggtcc tgagcacggc cccgatgccc agcacgaaca cgaagtacac cgcgataatg
     2101 aagtagtcga gccatgactc gttgacctcg atcaatggct cctgcaagaa cgccaggttc
     2161 aacaacttga ccatctcctt ccccgcaacg gttcccctac cgtattgtcc ctaacggctc
     2221 tgcttcgtcg ctactcggtc agagtcccaa ccacctccga atgagatctc ccgcagatga
     2281 tcgcggtcgc cggcggcatg ttttccgggc acgcttcgac aacgataacg ttcggttgca
     2341 tcaatgccat ttacgctcct tcctctccct gatagcggaa gtaaataccc ttgccacgcc
     2401 agcgtcaaat tgaagctttt ctctagcggc gcagccctag gggagaggac cggagtcaga
     2461 ccaacgcgtg ggacggagga gagaatgttg ccagaatcac cgggggcgaa acgccgcgcg
     2521 gaggtacgag agcgccacgg ccccgggccg tggcgctgtg ggcaaacggt atcttgccag
     2581 ggtgcggtcg gttagaccgt caccaaccgc tgcggctcag aacggcatcg agatgaacgg
     2641 cgaggagtag tcgggggcgc gggctagcac ctcttcagcc tcttcgcgcg acaacctgtt
     2701 cttctcggcc tcgaggatca tcggcacaag gccaacgtcg ccggccatgg cgaggccggt
     2761 gacctcctcc tgggagagaa tccaggagac ggcggcgtct atgtactcct gctgatcgaa
     2821 cggctcgtac caggtggcat accgctggtc gccggtaggg tcgccctcgg gccagttgcg
     2881 gcgcgagatg gtcttgatga tgagcagtcc agcgtcctga cgcttcacct cgtcgaccag
     2941 ggcgttgtag tccgcccggt aaccctcgtc cgtggagagg atgaagttcc agggcgtgag
     3001 gaccgtgtcg aacgggaagc gcctgagggc ctcgagatgg gtcgaggggg cgccgttgcc
     3061 gtggcccgtg atgccgatgc cccccacgag gccttcctcc ttagcctcga tggccgcctc
     3121 aagcgaaccg ccagagcccg tggcgcgatc gaggtcttcg aggtcaccca cggcgtgcaa
     3181 ttgcaggagg tccacgttat ccacgcgcag gcgctcgagg gaggcctcga tctggcgcct
     3241 cgcagcatcc ttctcgcgca gacccgtctt tgtagagagg aagatacggt cccggatctc
     3301 acccatccaa cgtccgtact ccagctcgga gtccccgtag tcggccgccg tgtcgaagtg
     3361 gttcaccccg gcgtcgagcg cctgctgtat ggccctgtca gagtcctcct cgttcgtctc
     3421 cgagagcgcg gccccgccga aggtgagcac ggagttctcc cgaccaagac gtccaagacg
     3481 acgatattcc attcccggct cctctctcag cttccccggc tacctgtatc cttttcccat
     3541 tcgatccgca acgtaaccac ctcgaaaggc cttaccgcga ggcgcacggc gccatcgtgc
     3601 acctcgaccg cgccctcgtc ctcttcgagg aggctggtgc ggtggacgct tccgggagcc
     3661 tcggcgaacc tgaggacgca ctcgcccctc gcgccgttcg gctcgtagag gcgcaggatt
     3721 cctccccgcc cgtcctcggc cggcttgagt gccccgagcg cgagctcgag cccttcggct
     3781 tccaggagcc cgtacgcggc ggggtcagtc cggccctctg taacgatgag cgggctgttg
     3841 agccggtggg cttcactggc gacccccgcc tcggtccagt cgccggggtg ggggaagagg
     3901 ctgtaggtga agcggtgctc tccctggtcg gcaagggggt cggggtagag cggggcgcgg
     3961 aggaggctga tgccgagaac attgtccctt gcgctgtgac cgtacttgcc atcattgagg
     4021 agggctacgc cgtatccagg ctcggagagg tcggcgaagc ggtgcgcgca gacctcgaag
     4081 cgcgcctcgt cccaggaggt gttgcggtgc gtagggcgcc tcaccacgcc gtgcatggtc
     4141 tcgaaggtcg cctcgtggga acggacacgc agcgggaaaa gcgcccgcag gagtacctgc
     4201 cgctcgtgcc accacacgtg
//
LOCUS       DBNGVD010000036         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_370144, whole genome shotgun sequence.
ACCESSION   DBNGVD010000036 DBNGVD010000000
VERSION     DBNGVD010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_370144"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="AC16CM_00945"
     CDS             <1..975
                     /locus_tag="AC16CM_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3138422.1"
                     /translation="TVFIGVLAFVAQWSRKNRMAEISLVVVLAFISLLLLATGALLTA
                     VWIWGQAPPEIQRLLEVSSLPILVSGLAGLALCFPTLRKIVRREPNGPFWTNPPVFFG
                     LWMFATVLLSNNLIGILGLEQLNEIDAFSLGTGGRISPLTVLASQLPFLVVAIVGVGA
                     GIRRGPRETLARLGYGPISVRALGVVVLFIAVAFALSVAADYLFSVLQPDLYRRVGEV
                     SGTLFDPKGLSLVSAVLFALLIGLGAGLGEETLFRGAVQPVFGIGATSVLFASMHVQY
                     GPSVLLVYVFVLSVGLGLLRRYFNTTTCFLAHAGYNTLGILGVYFFGL"
     gene            complement(978..1469)
                     /locus_tag="AC16CM_00950"
     CDS             complement(978..1469)
                     /locus_tag="AC16CM_00950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138423.1"
                     /translation="MRFTLTQITRADAEAISRWRYRGPYSIYDGSPEVVASLLEPRYL
                     YHAVHDKSGGLVGYFCFGEDARVSAGRTSGLYDREDALDVGLGMRPDLTGKGLGEEFV
                     RAGLQFADGNYSPAAFRLTVATFNQRAISVYERVGFERVGAFGATKLNGEREWLLMRR
                     APG"
     gene            complement(1490..1789)
                     /locus_tag="AC16CM_00955"
     CDS             complement(1490..1789)
                     /locus_tag="AC16CM_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3138424.1"
                     /translation="MKTLEISPERHKGDLLSTIGETLRAERRERGLTLKQVAEGAHVS
                     VSYLAEIERGEKDPSSRVLESIAAGLDVEVSELLVRIAAALEPATVVPVTESLAA"
     gene            complement(1812..>2375)
                     /locus_tag="AC16CM_00960"
     CDS             complement(1812..>2375)
                     /locus_tag="AC16CM_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3138425.1"
                     /translation="ALGVDLVVVHAGSHGGDGEEVGMDRLVEGLVSAREQAAEFDGAS
                     GVAEVIVENSVGAGTQLCSTFGALGEVARRAGVRVCVDTAHVFVAGYDLTSGEGGEKL
                     ASDLVAAVGDAVALLHLNDAKNELGSHRDGHRKVGEGCISIEAWPGLFGGLPGVPVIM
                     ETPYETPHADLEEILLVKKLAGGLRKA"
BASE COUNT          346 a          837 c          711 g          481 t
ORIGIN      
        1 acggtcttta tcggcgttct cgcgttcgtt gcgcaatggt cgcggaagaa tcgaatggcg
       61 gagatctcgc tggtcgtggt gcttgctttc atctcgctcc tgctcctggc cacgggtgct
      121 ctgctcactg cggtctggat ctggggccag gcgcccccgg agatccagcg gctcctggag
      181 gtttcgagcc tgcccatcct ggtctcgggc ctggccggtc tggcgctctg cttcccgacg
      241 ttgcgcaaga tcgtacgccg tgaacccaac gggcccttct ggacgaaccc gccggtattc
      301 ttcggcctgt ggatgttcgc caccgtgttg ttatccaaca acctgattgg gatcctcggc
      361 ctcgagcaac tgaacgagat cgacgctttc tcgcttggca cggggggcag gatctcaccg
      421 cttaccgtgc ttgcgagcca gttacccttc ctcgttgtcg ccatcgtcgg cgtcggcgcg
      481 gggatacgtc ggggcccccg cgagacgctt gcccgcctcg gctacggacc catctccgtc
      541 cgcgctctcg gcgtggtggt gcttttcatc gcggtggcat tcgccctctc ggttgccgcc
      601 gactacctct tctccgttct ccagcccgac ctataccgca gggtcggcga ggtctctgga
      661 acgctcttcg atccgaaggg ccttagcctg gtctccgcag tgcttttcgc cttgctgatc
      721 gggctcggtg ccgggctggg ggaggagacg ctgtttcgcg gcgcggtgca acccgtcttc
      781 ggcatagggg cgacctcggt tctgttcgcc tcgatgcacg tccagtacgg gccctctgtg
      841 ctcctcgtct acgtcttcgt gctctctgta gggcttgggt tactccgccg ctacttcaac
      901 accacgacat gcttcctggc ccacgccggc tacaacaccc tgggcatcct gggcgtctac
      961 ttcttcggtt tgtagcttca gccgggggcg cgcctcatca acagccactc cctctctccg
     1021 ttcaacttgg tcgcgccgaa ggccccgacg cgttcgaaac ccacgcgctc gtagacgctg
     1081 atggcccgct ggttgaaggt ggccacggtc agccggaacg ccgcgggcga gtagtttcca
     1141 tccgcgaact gcaggccggc gcggacgaac tcttctccga ggccctttcc cgtaagatct
     1201 ggcctcatcc cgaggcccac gtccagcgca tcttctcggt cgtagaggcc cgaggtcctg
     1261 ccagccgaaa ccctggcgtc ctctccgaag cagaagtacc cgaccagccc tccggatttg
     1321 tcgtgtaccg cgtggtagag gtagcgtggc tccaggagcg aggccactac ctcggggctc
     1381 ccatcgtaga tcgagtaagg ccctcggtag cgccagcgcg agatcgcctc ggcgtcggcg
     1441 cgggtgatct gggtcagggt gaagcgcact tctggcgcgg ggagaagctc taggcggcca
     1501 gggactctgt aacggggacg accgtcgcgg gctccagggc ggcggcgatc ctgacgagta
     1561 gctcgctgac ctccacgtcg aggcccgcgg ctatgctctc aaggaccctc gaagaggggt
     1621 ctttctcgcc gcgttcgatc tccgcgagat aggacaccga gacgtgcgcg ccctctgcga
     1681 cctgcttcag cgtgaggccg cgctcgcggc gctcggcccg cagggtctcc ccgatggtcg
     1741 agagaaggtc tcctttgtgc cgctccgggc ttatctctag agtcttcaca tcctagattc
     1801 tatcttcccg tctaggcttt gcgcaaccca ccagccaatt tcttgacgag gaggatctcc
     1861 tccaggtcgg catggggcgt ctcgtagggc gtctccatga tcacgggcac cccgggcaaa
     1921 cctccgaaaa gccccggcca cgcctcgatg gagatgcatc cctcccccac cttgcgatgc
     1981 ccgtcacgat ggcttccgag ctcgttcttc gcgtcgttca ggtggagcag cgcgaccgca
     2041 tcacccacgg cagcgaccag gtcggaggcg agtttctctc ccccctcccc cgacgtcagg
     2101 tcgtagccgg cgacgaacac gtgggccgtg tccacgcaca cccgaacacc ggcgcgcctt
     2161 gcgacctccc cgagcgcacc aaaggtggag cagagctggg ttccagcccc cacggagttc
     2221 tccactatta cctcagccac cccagaggcg ccgtcgaact ctgccgcctg ctccctggcg
     2281 ctcaccagtc cctccaccag ccggtccatg ccaacctctt cgccgtcacc gccgtgactg
     2341 ccagcgtgca cgaccaccag gtcgacgccc agggc
//
LOCUS       DBNGVD010000037         4103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4560444, whole genome shotgun sequence.
ACCESSION   DBNGVD010000037 DBNGVD010000000
VERSION     DBNGVD010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4560444"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16CM_00965"
     CDS             complement(<1..710)
                     /locus_tag="AC16CM_00965"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3138426.1"
                     /translation="MDYRDTEGKPKEACGVFGIYSRELAGELAQRTYFGLYALQHRGQ
                     ESAGIAVSDGERTTAMRDMGLVSQVFDRTSLAALEDGHLSLGHVRYSTTGSASWENAQ
                     PEFGGRGDVNVAVAHNGNLVNAGKLRDGLAEDGFRFYSTSDTTYIAAAAVRELERGLS
                     VGEAVQAAMRHFEGAYSVAMIFRDKLVAFRDPHGFRPLSIGEVDGGYAVSSETCGLDI
                     IGAKFLRDVEPGEVVVMD"
     gene            complement(736..1104)
                     /locus_tag="AC16CM_00970"
     CDS             complement(736..1104)
                     /locus_tag="AC16CM_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138427.1"
                     /translation="MDSADKVNVAQKFSLFEEHWSPKVVGELNGQHVKLVKISGEFVW
                     HHHDDEDEMFLVVDGRFRMEFRDREVWIEEGEFIVVPRGVEHRPVAEEEASVMLFEPA
                     STLNTGNVKGEMTVAELDRI"
     gene            complement(1097..3328)
                     /gene="purL"
                     /locus_tag="AC16CM_00975"
     CDS             complement(1097..3328)
                     /gene="purL"
                     /locus_tag="AC16CM_00975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3138428.1"
                     /translation="MNTQETYSSLGLSDFEYDRILELMGRPPNYLELSLFSVMWSEHC
                     GYKNSRPLLKRFPNEGERVVQGPGENAGVVEVGDGWAVAFKMESHNHPSAVEPYEGAA
                     TGVGGIIRDILAMGARPVALLDSLRFGPLGDERSRYLFREVVRGIGGYGNAVGVPTVG
                     GEVEFAEAYSGNPLVNAMCVGVIRTEELVHSSATGEGNLVILLGSKTGRDGIHGATFA
                     SDELTEESESKRSNVQVGDPFCEKMLIECCLKLLEEDLLVSLQDLGAAGITSSASEMA
                     AKGGVGIEIETDRVPLRESGMEPWEVMISESQERMLAVVEPDKADEVLALAERYELVG
                     AVVGRVADHGDLRIIHHGEIAGTVPAGLLADAPVYRREVVRPSYLDEVQELDLESLPE
                     PEDYNAVLLKMLAHPNLRSRRSIFTQYDHQVGTDTVVLPGADAAVMRIKGTELGFAVT
                     TDGRGRHCYLDPRGGGAATVAEAYRNLSCVGAEPVAVTDCLNFGSPEKPDGYYQLAEC
                     IEGMAEACEVLGTPVVSGNVSLFNETDRGAIYPTPTVGMVGVMKDVSRHATPAFKREG
                     DRVMIVGEPTAYSLAGSEYLQIMGDNVAGRPPGPDITTEKTYSDLIRSFIERGLIDTA
                     HDVSGGGQAVALSEMALASGIGFEYDGEVEPEELLQDGRRDVTLFGECGARFLIAFPG
                     ERWDAVQEALNVPWVAYDLLGYTGGDRFKIFDFIDVGLDELGEAYERDLFEAHAPEGG
                     HIG"
     gene            complement(3325..3987)
                     /gene="purQ"
                     /locus_tag="AC16CM_00980"
     CDS             complement(3325..3987)
                     /gene="purQ"
                     /locus_tag="AC16CM_00980"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3138429.1"
                     /translation="MRIGVTVFPGSNCDRDALYGVERAGAEAVELWHADADLKGVDAV
                     IVPGGFSYGDYLRPGAIARFAKVMGSLERFARDGGPILGVCNGFQVLTEAHLLPGALL
                     RNTHMRFVCDRVRVRVETVDTPWTAACDPGEELTLPVAHNEGNYFADSETLARLEDEE
                     RVVLRYLENPNGAAHDIAGVCNEGRNVVGLMPHPERVSDPTLGSDEGLRILRSVLVGA
                     GV"
     gene            complement(3984..4079)
                     /locus_tag="AC16CM_00985"
     CDS             complement(3984..4079)
                     /locus_tag="AC16CM_00985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3138430.1"
                     /translation="MEVESADEVDAMCRRLLSNPTIEEYEWEIVS"
BASE COUNT          706 a         1433 c         1219 g          745 t
ORIGIN      
        1 tcgtccatga ccacgacctc cccgggctcc acgtcgcgca ggaacttcgc gccgatgatg
       61 tccaggccgc aggtctcgct ggagacggcg tagccgccgt cgacctcccc gatggagagc
      121 ggacggaagc cgtgcggatc gcggaacgcg acgagcttgt cgcggaagat catggccacg
      181 gagtaggcgc cctcgaagtg gcgcatggcc gcctgcacgg cttctcccac ggacaggccg
      241 cgctcgagct ctctcaccgc cgcggcggca atgtaagtgg tgtccgaggt ggagtagaac
      301 ctgaagccgt cctcggccag gccgtccctt agcttgccgg cgttcaccag gttgccgttg
      361 tgggccacgg caacgtttac gtccccccga ccgccgaact cgggctgggc gttctcccag
      421 gaggccgagc ccgtcgtgga gtagcggacg tggcccagcg acaggtggcc gtcctccagg
      481 gcagcgaggc tcgtgcggtc gaacacctgc gagaccaggc ccatgtcgcg catggcggtc
      541 gtgcgctctc cgtcagagac ggctatgccg gccgactcct ggccgcggtg ctgcagcgca
      601 tagaggccaa agtacgtgcg ctgggccagc tctccggcca gctcgcggga gtagatcccg
      661 aagacgccgc acgcttcctt cggcttgcct tccgtatccc ggtaatccac gtctggagca
      721 cccaccttcc gagcctcaga tcctgtccaa ctcggcgacc gtcatctcgc ccttcacatt
      781 gcccgtgttc agcgtggatg cgggctcgaa gagcatcacg ctcgcctcct cctccgcgac
      841 gggcctgtgc tcgaccccgc gcggcaccac gatgaactct ccttcctcga tccatacctc
      901 ccggtccctg aactccatcc tgaaacggcc atcaaccacc aggaacatct cgtcctcgtc
      961 gtcgtgatgg tgccagacga actcgccgga gatcttgacg agcttgacat gctgtccgtt
     1021 caactcaccc actactttcg ggctccagtg ctcctcgaaa agcgagaact tctgcgcgac
     1081 gttgaccttg tctgcgctat ccaatgtgcc cgccttccgg cgcgtgcgcc tcgaagaggt
     1141 cacgttcgta ggcttccccg agttcatcca gaccaacgtc gatgaaatcg aagatcttga
     1201 acctgtctcc accagtgtag ccgagtaggt cgtaggcgac ccatggcaca ttaagtgctt
     1261 cttgcaccgc gtcccacctt tcgcccggga aggcgatcag aaaacgagcg ccacattctc
     1321 cgaagagggt cacgtccctc cggccatctt gcagtagctc ctcgggctcc acttctccgt
     1381 cgtactcgaa accgattccg ctagccagag ccatctcaga aagcgccacg gcctggccac
     1441 cgcctgagac atcgtgcgcc gtatcaatca ggccacgctc aataaagctg cggattagat
     1501 ccgagtatgt cttctcggtg gtgatatctg gtcctggagg tctgccggcc acgttgtcgc
     1561 ccatgatctg caggtactcc gaaccggcca acgagtaagc ggttggctca ccaactatca
     1621 tcacccgatc cccttcacgc ttgaaggcag gcgtcgcgtg ccggctcacg tccttcatca
     1681 ccccgaccat ccccacggtc ggggtcgggt agattgctcc gcggtctgtc tcgttgaaga
     1741 ggctcacgtt gccgctaacg accggcgtcc ccagcacctc gcaggcctcg gccattccct
     1801 cgatgcactc ggcgagctgg tagtagccgt cgggtttctc ggggctgccg aagttgagac
     1861 agtcggtgac ggccacgggc tcggccccga cgcacgagag gttcctgtac gcctccgcca
     1921 cggtggctgc tcctccaccg cgcggatcca ggtagcagtg gcggccccgt ccatcggtgg
     1981 tgacggcgaa gccaagctcc gtgcccttga tcctcatgac cgccgcgtcg gcaccgggga
     2041 gcaccacggt gtccgtgccg acctggtggt cgtactgggt gaagatggac ctccgtgaac
     2101 gcaggttcgg atgtgccagc atcttcagca gcacggcgtt gtagtcctcc ggctcgggaa
     2161 gactttccag gtccaactcc tgcacctcat cgaggtaaga cgggcgtacc acctcgcgtc
     2221 tgtagacggg ggcgtcggca aggagtccgg ctggcacggt accggcaatc tctccgtggt
     2281 ggatgatgcg cagatcgccg tggtcggcga cgcggcccac taccgcacca acaagctcgt
     2341 agcgttcggc cagggcgagg acctcatcgg ctttatccgg ctcgacgacg gccagcatcc
     2401 gttcctggga ctcggagatc atgacctccc acggctccat cccgctctcc cgcaacggta
     2461 ccctatccgt ctctatctcg attccgaccc cgcccttggc ggccatctca gaagcggaag
     2521 aagtgatgcc ggcggcaccc agatcctgca acgagacgag caggtcctcc tccagaagct
     2581 tgaggcaaca ctcgatgagc atcttctcgc agaacgggtc gcctacctgg acgttggatc
     2641 tcttggactc cgactcttcg gttagctcgt cggaggcgaa ggtggctccg tggatgccgt
     2701 ctcgtccggt cttggagccg agcaggatga cgaggttacc ttcaccggtt gcgctggagt
     2761 ggacgagttc ctccgtccgg atcactccaa cgcacatggc gttgacgagc gggttacccg
     2821 agtaggcctc cgcgaactcc acctccccgc cgaccgtggg caccccgacg gcgttgccgt
     2881 accccccgat gccgcgcacg acctccctga agaggtagcg gcttcgctca tccccgagcg
     2941 gcccgaaccg gagggaatcg agaagcgcca cggggcgcgc gcccatggcc aggatgtccc
     3001 ggatgatgcc gccaacgcca gtagcggccc cctcgtaggg ctcaacggcc gagggatggt
     3061 tgtggctctc catcttgaag gcgacggccc acccgtcccc cacctcgacc acgccggcgt
     3121 tctcccccgg tccttgcacg acccgctccc cctcgttcgg gaagcgcttg aggagcgggc
     3181 gcgagttctt gtatccgcag tgttcgctcc acatcacgga gaagagcgag agctccaggt
     3241 agttcggggg ccgccccatc agctccagga tgcgatcgta ctcgaagtcc gagaggccca
     3301 gtgagctgta tgtctcctgg gtgttcagac cccggccccc accagcacag acctcaggat
     3361 cctcaacccc tcgtccgacc cgagcgtggg gtcggatacc ctctccgggt gcggcatgag
     3421 cccgaccacg ttgcggccct cgttgcaaac gccggcgata tcgtgggcgg caccgttcgg
     3481 gttttccagg tagcgcagca ccacacgctc ctcgtcttcg agcctggcca gcgtctcgga
     3541 gtcggcgaag tagttgccct cattgtgggc cactggcagg gtcaactcct cgccgggatc
     3601 acaggccgcg gtccacggcg tgtccacggt ctccacccga acgcggacac ggtcgcacac
     3661 gaagcgcatg tgcgtgttgc gaagcagggc gccgggcagc agatgcgcct ccgtcaggac
     3721 ctgaaagccg ttgcagacgc cgaggatcgg ccccccgtcc ctggcgaacc tttcgagcga
     3781 gcccatcacc ttcgcgaagc gggcgatagc gccgggcctg aggtaatcac cgtaggagaa
     3841 accgcctgga actataacgg cgtcgacgcc cttgagatcg gcgtccgcat gccagagctc
     3901 taccgcctcg gctcccgccc gctccacgcc gtagagcgcg tcgcgatcgc agttggagcc
     3961 tgggaagacc gttacaccta tcctcaagag acgatctccc actcgtactc ctcgatcgtc
     4021 gggttggaga ggagcctccg gcacatcgcg tcaacctcgt ccgcgctctc gacctccatc
     4081 tcgacgagcc ttccgatatg aac
//
LOCUS       DBNGVD010000038         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2738505, whole genome shotgun sequence.
ACCESSION   DBNGVD010000038 DBNGVD010000000
VERSION     DBNGVD010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2738505"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="AC16CM_00990"
     CDS             complement(<1..361)
                     /locus_tag="AC16CM_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138431.1"
                     /translation="MEYRNLGRTGVKVSELCLGCMMFGGRTGDDDSADIIDRAIDEGI
                     NFLDTANAYSRGASEVAVGKALKRNGKRDRIVLATKVHIPMDDDDPNASGNHRRHIME
                     QCEASLERLGVDHLDLYQ"
     gene            508..1533
                     /locus_tag="AC16CM_00995"
     CDS             508..1533
                     /locus_tag="AC16CM_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3138432.1"
                     /translation="MRVVQAGLGVWGRSWTGVVRQAPGVELTAVVDPDRSALGHVDAI
                     PGYASLEDALAEVGCDAVLVASPPRSHHAVAKTALEAGKHVLCEKPLATGLGEAFDLV
                     RTAERAECYALVSQNYRHNAPFRAVQRVVREGRIGDLVSIYIGCLRDTRDLFEPDDFR
                     YSMRHPYVLDMAIHHFDLIRAATGREVRGVFARSRRVPDTPFVHDPAVAAVLDLDGGV
                     PVVYEGDWATRGPETSWNGQWEIVGEEGRLLWRGDTEDRGEGEVLLERWGEETLSVGQ
                     VNQTFSERAATLQMLREAVEEGRPPETTATDNVKSLAVVLGCVRSIESGERVDVPALL
                     AAGAANL"
     gene            1589..2590
                     /locus_tag="AC16CM_01000"
     CDS             1589..2590
                     /locus_tag="AC16CM_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3138433.1"
                     /translation="MARPVTLFTGQWADLPLEELARKCGEWGFDGLELACWGDHFDVD
                     RALSEDGYVEERWEILRSNNLSCWSISNHLVGQAVCDPIDGRHRATLPPDVWGDGDPE
                     GVRRRAAEKIKDTARAAARFGVTQVNGFTGSSIWHMLYSFPPNDFAEVERGYEDFAQR
                     WNPIIDVFDAEGVRWGLEVHPTEIAYDFVTTRKALDAIGNREGFGINFDPSHFAHQFL
                     DSSAFIEEFADRIYHFHIKDSRKRLDGRRSILGGHLDFGSPERGWDFVSPGHGDVNLE
                     DCIRALNRIGYGGPLSIEWEDSGMDREYGAQDALAFARRTDFEPSTQAFDAAFAREE"
     gene            2593..3759
                     /locus_tag="AC16CM_01005"
     CDS             2593..3759
                     /locus_tag="AC16CM_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3138434.1"
                     /translation="MAEIGVGLVGYKFMGRAHSNAYRQVGRFFDVDPAPRMNAICGRD
                     EAGVSEAARTLGWESYETDYRRLIARDDIGLVDVVTPGNTHREIVLAALEAGKHVLCE
                     KPLANDLAEAREMLEAARNAGTVNMVCFNYRRAPAVQLARKLVADGRLGEIRHWRATY
                     LQDFIMEPDFPLIWRLQKEVAGSGALGDIGAHIVDLAQFVVGPIAEVTGMTETFIGER
                     PLEEEGPGGGLSATGGTRMGRVTVDDAAAFLARFENGAVGTFEATRFAAGRRNQNSFE
                     VNGSKGSVAFDLERMNELEVFFTEDDPDVQGFRTVNVTEPDHPYTGAWWPPGHILGYE
                     HTFIHTMKDLMDGISAGESPAPTFEDGYRCQAVLDAVERSAASGKWTRPETEGI"
     gene            3761..>3935
                     /locus_tag="AC16CM_01010"
     CDS             3761..>3935
                     /locus_tag="AC16CM_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR3138435.1"
                     /translation="MASSTRVTIWNEYRHEKNDEHVAEIYPEGIHGVLAAVLKDAGLE
                     VRTATLDEPEHGLT"
BASE COUNT          705 a         1205 c         1359 g          666 t
ORIGIN      
        1 tctggtagag gtccaggtgg tctaccccga ggcgctccag cgatgcctcg cactgctcca
       61 tgatgtgcct gcggtggttg cccgaggcgt tcgggtcatc gtcgtccatg ggaatgtgga
      121 ccttggtcgc gagcacgata cggtcgcgct tgccgttccg cttcagggcc ttgccgaccg
      181 ccacctcgct agcccccctg ctgtacgcgt tcgccgtatc cagaaagttg atgccctcgt
      241 cgatggcacg gtcgatgatg tctgccgagt catcgtctcc cgtcctgccg ccgaacatca
      301 tgcacccgag gcacagctcg ctgaccttta caccggtccg tccgaggttc ctgtattcca
      361 cgtgtcgctc cttcgaggta gccgtctacc cgtgcaaagt agacgtatac cctttgagcg
      421 ggcatcgtaa cgcctaccca cgtttgatcc caccaggcgt agcgtgtagc atcgcggcct
      481 gtaaactgtg gagagggagg gggcgacatc cgcgtagtcc aggccggact cggtgtctgg
      541 ggccgtagtt ggaccggtgt ggtgcggcaa gcccctggtg tcgaactgac cgccgtggtc
      601 gacccggaca ggagcgcgct cggacatgta gatgctatcc ccggctacgc atccctggag
      661 gacgcgttgg cggaggtcgg gtgcgatgcg gtgctcgttg cctcgccgcc ccggtcacac
      721 cacgcggtgg cgaaaaccgc gctcgaggcg ggcaaacacg tgctttgtga gaagccgctg
      781 gccacgggcc tcggtgaagc ctttgacctg gtaaggaccg cagaacgggc ggagtgttac
      841 gccctcgtca gccagaacta ccgccacaac gcgccgttcc gtgccgtgca gcgggtcgtt
      901 cgggaaggcc ggatcgggga tctggtctcg atctatatcg ggtgcctgcg tgacacgaga
      961 gaccttttcg agccggatga tttcaggtac tccatgcgcc acccttacgt tttggacatg
     1021 gccatccacc acttcgacct cataagggcc gcgaccggcc gcgaggtgcg cggcgtgttc
     1081 gcccgtagcc ggcgcgtccc cgacacccct tttgttcatg atccggcggt ggcagcggtc
     1141 ctggatctcg acggtggcgt gcccgtcgtc tacgagggag actgggcaac ccgcggtccg
     1201 gagacttcct ggaacggcca gtgggagatc gtcggcgagg agggacgctt gctctggagg
     1261 ggggacacgg aggaccgcgg cgagggagaa gtgttgttgg agaggtgggg cgaggagaca
     1321 ctttccgtag gacaggtaaa ccagacgttc tcggagaggg cggcgacgtt gcagatgctc
     1381 cgcgaggccg tcgaggaggg taggccgccg gagaccaccg ccacagacaa cgtgaagagc
     1441 ctggccgtcg tgctcggctg tgtccggtcc atcgaaagtg gcgagagggt ggatgtgccc
     1501 gccctgctgg cggcgggcgc cgccaacctg tagggtccta tcccagaggt tggtatcctg
     1561 cgagagatcg aaaaggggag gacctgacgt ggccagaccg gtaacgctct ttacgggaca
     1621 gtgggccgac ctgccgttgg aggagctcgc caggaaatgc ggtgagtggg gcttcgacgg
     1681 gctcgagcta gcgtgctggg gggatcactt cgacgtagat agggccctct cggaagacgg
     1741 ttacgtggaa gaacgctggg agatactgcg ctcgaacaac ctgagttgct ggtctatcag
     1801 caaccacctc gtcgggcagg cggtctgcga tcccatcgac ggccgccaca gggccacgct
     1861 accgcccgac gtgtggggtg atggggaccc ggagggcgtg cgccggcgtg cggcggagaa
     1921 gatcaaggac accgccaggg ccgcggcacg tttcggggtg acgcaggtca acgggttcac
     1981 cgggtcgagc atctggcaca tgctctactc gttcccaccc aacgacttcg ccgaggtgga
     2041 gcggggctac gaggacttcg cccaacggtg gaacccgatc atcgacgtgt tcgacgcgga
     2101 gggcgtgagg tggggcctcg aggttcaccc gaccgagata gcctacgact tcgtcacaac
     2161 acggaaggcg ctcgacgcta tcgggaacag ggaaggtttc ggcatcaact tcgacccgag
     2221 ccacttcgcc caccagttcc tcgattcctc cgccttcatc gaggagttcg cggaccgcat
     2281 ctaccacttc cacataaagg acagccgcaa gcgcctcgac ggccgcagga gcatcctcgg
     2341 ggggcaccta gacttcggca gcccggagcg cggctgggac ttcgtctcgc ccggacacgg
     2401 cgatgtgaac ctggaagact gcatccgggc gctcaaccgt atcggctacg ggggacccct
     2461 gagcatcgag tgggaggact ctgggatgga ccgtgagtac ggggcacagg atgccctggc
     2521 gttcgcccgg cgcacggatt tcgagccgtc gacgcaggcc ttcgatgcgg ccttcgcgag
     2581 ggaggagtag gcatggctga gatcggggtc gggcttgtcg gctacaagtt catgggccgt
     2641 gcccactcga acgcctaccg ccaggtcggg cgcttcttcg acgtggatcc cgcaccacgc
     2701 atgaacgcca tctgtgggcg cgacgaggcc ggcgtgagcg aggccgccag aaccctcggc
     2761 tgggagtcct acgagacgga ttaccgcagg ctcatcgcgc gggacgacat cgggctggtc
     2821 gacgtagtta cgcccggcaa cacgcaccgg gagatcgtgc tcgccgccct cgaagcgggc
     2881 aagcacgtgt tgtgcgagaa accgctggcg aacgacctcg ccgaagcccg cgagatgctc
     2941 gaggcggcca ggaacgccgg gaccgtcaac atggtctgtt tcaactacag gcgggcgccc
     3001 gcggtgcagc tggcgagaaa gctcgtggcc gacgggcggc tgggggagat caggcactgg
     3061 cgggctacgt acctgcagga cttcatcatg gaaccagact ttccccttat ctggcgcctt
     3121 cagaaggagg tcgccggctc cggcgccctc ggcgatatcg gcgcccatat cgtcgacctc
     3181 gcccagttcg tggtcgggcc catcgccgag gtcaccggga tgaccgagac gttcataggg
     3241 gagcggccac ttgaggaaga gggaccaggg ggcgggctct ccgcgaccgg cggcacgcgg
     3301 atggggcgtg tcacggtgga cgatgctgct gccttcctgg cgcgcttcga gaacggtgcc
     3361 gttgggacct tcgaggccac gcgcttcgcc gcgggccgca ggaaccagaa ctccttcgag
     3421 gtcaacggct cgaagggtag cgtcgctttc gacctggagc ggatgaacga gctagaggtt
     3481 ttcttcaccg aagacgatcc cgacgtgcag ggcttccgca cggtcaacgt gacggagccg
     3541 gatcatccct acaccggagc ctggtggccg ccggggcaca tcctgggtta cgagcacacc
     3601 tttatccaca ccatgaaaga cctgatggac ggcataagtg ccggggagag cccggcgccg
     3661 acgttcgagg acggctatcg ttgtcaggct gtcctggacg ccgtcgagcg gagcgccgcc
     3721 agcggcaaat ggaccagacc agagacggaa ggaatctaac ttggcgtcct cgacgcgcgt
     3781 aacgatctgg aacgagtaca ggcacgagaa gaacgatgaa cacgtcgccg agatctaccc
     3841 cgagggcatc cacggcgtgc tggccgcggt gctgaaggat gccggcctgg aggtccgtac
     3901 ggcgaccctg gacgagcccg agcacgggct taccg
//
LOCUS       DBNGVD010000039         3139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4742918, whole genome shotgun sequence.
ACCESSION   DBNGVD010000039 DBNGVD010000000
VERSION     DBNGVD010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4742918"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="cbiT"
                     /locus_tag="AC16CM_01015"
     CDS             <1..500
                     /gene="cbiT"
                     /locus_tag="AC16CM_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HIR3138436.1"
                     /translation="SATRAFVLSRLGPGVGDLIWDVGAGSGSVAIECARLGAATIAIE
                     RDAESCARVRRNTRRHGVSVQLVEGEAPRALHDLPEPDAVFVGGTGGEFEEIVKLCAV
                     SARRSVVLALVGLERVVPAARILEGCGLEVETTFLQTSRMKGVGDLHRLAAESAAFVV
                     SGERP"
     gene            497..2182
                     /gene="cobJ"
                     /locus_tag="AC16CM_01020"
     CDS             497..2182
                     /gene="cobJ"
                     /locus_tag="AC16CM_01020"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR3138437.1"
                     /translation="MIGLVAATANGRRNAAHLASRWPDARLYEGRAREALGLAWGECD
                     GIVLFLATGAAVRLVAPLLQDKHRDPGVVTVDDAARFAVALCGGHEGGANDLAAGVAD
                     TLGATPVLTTASDSLGIPALDSLGKNLGFRLEEGSDLAAVGAGVVSGKKVSLVSERRW
                     PVGPLPDNVVRTEEPEAPSILISDRLEEVPRPAVVYRPPSLVVGVGCSRGAGKEEILG
                     LIEASLQDAGLSPDSVAALASIDVKRDEAGLLEAAASLDVPVRFFPAERLSTADVPNP
                     SEVVRGAVGTPSVSEAAVLSSGAELVLEKRKSANVTVAVGRLPVRGRLALVSLGPGED
                     ALIPPLAREALAASELVVGLDQYVDRVRHLLRPGTRVLTPPLGNELERAELALSEAGA
                     GGSTALVSSGDVGVYAMASPVLEIAGDDVDVVVVPGITASQAAASLLGSPLGHDHCSI
                     SLSDLLTPWEVIQSRVRAAAEGDFVVSLYNPRSKGRDWQLGKVMEMLLEHRPPDTPVG
                     IVRDAYRPTERATITDLASLRAESVDMLTIVVVGSSQTRMVAGRMVTPRGYSL"
     gene            2179..2382
                     /locus_tag="AC16CM_01025"
     CDS             2179..2382
                     /locus_tag="AC16CM_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3138438.1"
                     /translation="MRSIFAVTNACAGCGACLKTCPEKAIRPAPRENGSPLIVLEDRC
                     SGCAECAEVCPVSACVEVVLEAE"
     gene            2379..2963
                     /locus_tag="AC16CM_01030"
     CDS             2379..2963
                     /locus_tag="AC16CM_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR3138439.1"
                     /translation="MRRVHPIETESYRILRGMVDLSHLGPLSRAVAERIIHASADLEY
                     ADGLVLDEAALERGVEALRGERPIVTDVGMVAAGITGRETRCFVSDPRARGLSENLGI
                     TRSAAGFRLAAEEVGGDAIWVVGNAPTALFWLLDLAVAPALVVGLPVGFVGAAESKDA
                     LAASGLPAVTNRGPKGGSAVAAAALNALIYYEEE"
BASE COUNT          458 a         1057 c         1107 g          517 t
ORIGIN      
        1 agtccgcgac ccgcgctttc gtgctctccc gccttggtcc cggagtgggg gatctgatct
       61 gggacgtggg tgccggcagc ggctcggtgg ccatagagtg cgcccggctc ggagccgcca
      121 caatcgccat cgagcgggat gccgagagct gcgccagggt ccgacgcaac accagacggc
      181 acggggtctc cgtacagctg gtcgagggtg aagctcccag ggccctgcac gacctccccg
      241 agcccgacgc cgtcttcgtc ggtgggacgg gtggtgagtt cgaggagatc gtcaagctct
      301 gcgccgtcag cgctcgccgc tccgtcgtgc tcgcgctcgt gggcctcgag agggtggtgc
      361 cggctgcgcg gatactcgaa ggctgtggcc tggaggtcga gaccacgttc ctgcagacct
      421 cccgcatgaa gggtgtcggg gatctgcacc ggctcgccgc cgagagcgcc gccttcgtcg
      481 tctcggggga gaggccgtga tcgggctagt agcagccacc gccaacggcc gccgcaacgc
      541 ggcccatctc gcgtcacgtt ggcccgacgc ccgcctctac gaaggccgag ctagagaggc
      601 cctcggcctg gcctggggcg agtgcgacgg gatcgtgctc tttctagcca ccggcgccgc
      661 cgttcgcctc gtagccccct tgctgcagga caagcaccgc gacccaggcg tggtaaccgt
      721 cgatgatgcg gccaggttcg ccgtcgccct ctgcggcggg cacgaaggtg gcgccaacga
      781 tctcgccgcg ggcgtcgccg acacgctcgg cgcgacaccc gtcctcacca ccgcgagcga
      841 ctcccttggc atcccagccc ttgactctct cggaaagaac ctcggctttc gcctggaaga
      901 gggttccgac ctcgcggcgg tgggggccgg ggtcgtctct ggtaaaaagg tgtctctagt
      961 ctccgagcgc cgctggccgg tcggcccact gccggataac gtcgtcagaa cggaggagcc
     1021 ggaggcgccc tctatcctga tatccgacag gctcgaagag gtgccccgtc ccgccgtcgt
     1081 ctaccgtccg ccctcgctcg tcgtcggggt gggttgcagc cgcggggccg gcaaagagga
     1141 gatcctcggc ctgatcgagg cctccctgca agatgccggg ctgtccccgg atagcgtggc
     1201 cgccctggcc agcatcgacg tcaagcgcga cgaagccgga ctcctggagg cggcagccag
     1261 cctggatgtg ccggtccggt tcttcccggc agaacgtctc tctacggctg acgtgccgaa
     1321 cccgtccgag gtagttcggg gggcggtcgg gacgcccagc gtctcggagg cggccgtgct
     1381 ctcctctggc gccgagctcg tattggaaaa gcgaaaatct gccaacgtaa ccgtcgccgt
     1441 cggacggctg cccgtgcggg gaaggctggc gctcgtcagc ctgggacccg gggaagacgc
     1501 cctgatccca ccgcttgccc gcgaggccct cgccgcctct gagctggtcg tcgggctgga
     1561 tcagtacgtg gaccgcgtca ggcacctgtt gcggcccggt acgcgggttc tgacgccccc
     1621 cctgggcaac gagctggagc gggctgagct ggcgctctcg gaggccggcg cgggcgggag
     1681 caccgcgctg gtgtcgagcg gcgacgtggg tgtgtacgcg atggcctctc ccgtgctgga
     1741 gatcgccggc gacgacgtgg acgtggtcgt cgttcccggc ataacggcgt cccaggcggc
     1801 ggcctcgttg ctcggctcgc ctctcgggca cgaccactgc tcgatcagcc tctccgacct
     1861 gctgaccccc tgggaggtta tccagagcag ggtaagggcc gccgccgaag gggactttgt
     1921 cgtctcgctg tacaaccccc gctcgaaggg ccgtgactgg cagctaggca aggtgatgga
     1981 gatgcttctg gagcaccggc cgccggatac gcccgtcggc atcgtgaggg atgcctatcg
     2041 cccgacggag cgggccacca tcacggacct cgcctcccta cgtgccgaga gcgtggacat
     2101 gctcaccatc gtggtggtgg gtagctccca gacccggatg gtcgccggcc gcatggtaac
     2161 gccgagggga tactcgcttt gagatccatc ttcgccgtga cgaacgcctg cgccggctgt
     2221 ggcgcctgtc tcaagacatg cccagagaag gccatccgcc cggctcccag agaaaacggc
     2281 tctcccctga tcgttctcga ggaccgctgc tcgggatgtg ccgagtgcgc cgaggtctgc
     2341 ccggtctcgg cctgcgtcga ggtggtgctg gaggccgagt gagacgcgtt catcccatcg
     2401 agaccgagag ctatcgcatc ttacggggga tggtcgacct gtcccacctc ggaccgctct
     2461 cccgcgccgt cgcggagcgg atcatccacg cctctgcgga cctggagtac gctgatggcc
     2521 ttgtcctcga tgaggcagcc ctggagcgag gcgtggaagc gctgcgaggt gaaaggccca
     2581 tcgtcacgga cgtggggatg gtggcggccg ggatcacggg ccgcgagacg cgatgcttcg
     2641 tctccgaccc ccgcgccagg ggtctgtccg agaacctggg catcacccgt tccgcggccg
     2701 gcttcaggct cgccgccgaa gaggttggcg gtgatgctat ctgggtggtg ggtaacgcgc
     2761 ccaccgccct gttctggttg ctcgatctag cggtcgcccc cgcgctcgtg gtcgggctcc
     2821 ccgtcggctt cgtcggagcg gccgagtcga aggatgcgct ggcggcgagc ggcctgccgg
     2881 ccgtcacgaa tcgggggccg aaaggcggct cggcggtcgc cgcggcagcc ctgaacgcgc
     2941 tcatctacta cgaggaggaa tagagtggac catacggtcg gacttgcgga agagaacgga
     3001 tcccacaggc acgaggtcgg ccctgcgctg ctggtcgtcg gccacggaag ccgggatcca
     3061 cgcggcgcac gggagtttca cgacctgatc gggctcgtgc accgaagaaa cccgtccctt
     3121 gcggtcgagg gaggcttta
//
LOCUS       DBNGVD010000040         4526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2010518, whole genome shotgun sequence.
ACCESSION   DBNGVD010000040 DBNGVD010000000
VERSION     DBNGVD010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2010518"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="AC16CM_01035"
     CDS             complement(<1..908)
                     /locus_tag="AC16CM_01035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3138440.1"
                     /translation="MEISRREFLRLGLAGGVALTFPFGAAACSGEGSTGKLLPSRAKL
                     PRPFQVPLPVPPVLRPARTEDGVDYYEMTQRVGKAEILPGLRTEVWGYEGIFPGPTIV
                     SRSGRRIMVRQTNDLPVPVSTHLHGGRTPPGSDGYPTDLIMPKGAGGHAMEGHGSMGM
                     GESGHFKDYTYPQRQRAATLWYHDHRMDFTGPQVWRGLAGFHLIHDDEEEALPLPTGE
                     RDVPLMICDRSFDADGSFMYPSLDPSLQERPGVEDDYMDGVLGDTILVNGAPWPVMEV
                     SSTRYRFRILNASNARRYELALRPGKT"
     gene            complement(917..1372)
                     /locus_tag="AC16CM_01040"
     CDS             complement(917..1372)
                     /locus_tag="AC16CM_01040"
                     /inference="COORDINATES: protein motif:HMM:NF040028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HIR3138441.1"
                     /translation="MVHTVKTLDDTARAGVGEKPSRKVARVGVSVAARLAMVSYAFLS
                     SVLASGVLLQVFLAGMGAFGADWSFHVTLAQFLGMVPLLMVPVAFLGRLPWALRLLPL
                     GAVVLIGSQYALAHAAVPVAALHPVNGFLILLTSLYIARRSWGLARREG"
     gene            complement(1563..2237)
                     /locus_tag="AC16CM_01045"
     CDS             complement(1563..2237)
                     /locus_tag="AC16CM_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3138442.1"
                     /translation="MLIRILAVLGVGVWCLFVMLLSVGFVIDRGLADVGVVWLAVFGI
                     GVVAPVLLLRQVGRLIEAHEASRRPAPGAREREKELLETLREGGEITPATAAMRTSLT
                     VDEASEMLEELARKGHLERQAEEGGTAYSLGESRRKALPNGGPALPRPDTNQATTRLE
                     DPLSQRELEVLDLLAMGKTNSEVARDLFVSVGTIKSHTGNIYRKLGARNRTEALARAR
                     ELDLIR"
     gene            2644..3129
                     /locus_tag="AC16CM_01050"
     CDS             2644..3129
                     /locus_tag="AC16CM_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR3138443.1"
                     /translation="MHKVNELLGRDILGQDTGEKRGTVKNLLFDEATTRVVALVVDGG
                     GFFGGTKTVRWEYVVNVGDVVMLAGGAPLDNPKEDPEIDALRGRQHHITGTNIVTEGG
                     EKVGEAADVLLDDAGVVRAYEVKRGVIRDLSGREQVPAEDVISSGPDAIIIRNLPETR
                     P"
     gene            complement(3220..>4526)
                     /locus_tag="AC16CM_01055"
     CDS             complement(3220..>4526)
                     /locus_tag="AC16CM_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rubredoxin-like domain-containing protein"
                     /protein_id="HIR3138444.1"
                     /translation="VGMATGAIIRYAIEKGYTVITTGVIDEVIKSILPPGAMESMRAI
                     GDQMREAEAMGEEKPIDSLFKDFDERTADKANGMNGNGMNGNGANGNGSEANGHKNGS
                     ANGANGNSSDVAAKQEIFESGRGGFGQSEDQGDIEGVSAEVREEIHRAAQGQDRFECD
                     VCRYVAKGKPARCPVCGSGPTHFQAVDAEIATTAGDENLNLSEVYDGRELHWTEDATT
                     LLDSLEEWQEKRRVKARVEKSALKKGYTTITRDYVAQQYREETGREAPEVKSGGCPVS
                     HAKEQVQEASGATCPIDHSAFQKAGTQVPEGGSKEFTWTDDAIERLDRAPKGFMRNIS
                     RNMTEKLARERGVTHIDLALVEDSLTGARTTMEDVITGKVSIADLARDSGEKIEAEDG
                     GAPHPPMTVTMVCTTCGEEMEGVQPPEECPVCGAPGEDFEQR"
BASE COUNT          778 a         1518 c         1432 g          798 t
ORIGIN      
        1 gtcttgcccg gccgcagggc gagctcgtag cggcgggcgt tcgaggcgtt caggattcga
       61 aagcggtacc tcgtgctgga gacctccatc accggccacg gcgcgccgtt gaccaggatc
      121 gtatccccca gaaccccgtc catgtagtcg tcctcgacgc caggcctctc ctgaagcgaa
      181 gggtccagag acgggtacat gaacgagccg tccgcgtcga aggaacggtc gcagatcatg
      241 agcgggacgt ccctctcgcc cgtgggaagg ggcaaagcct cctcctcatc gtcgtggatg
      301 aggtggaagc cggccagacc gcgccacacc tgagggcctg tgaagtccat gcggtggtcg
      361 tggtaccaga gcgtagccgc ccgctgccgt tgcgggtagg tgtagtcctt gaaatgtccg
      421 gactcgccca tgcccatcga gccgtggccc tccatcgcat gtcctcctgc ccctttcggc
      481 atgatgagat ccgtggggta gccgtcgctc cccgggggcg tacgtccccc gtgcaggtgg
      541 gtcgagacgg gcacggggag gtcgttggtc tggcgcacca tgatccggcg tccgctgcgg
      601 gacacgatgg ttggtccggg gaagatcccc tcgtagcccc acacctccgt cctgaggccg
      661 ggcaggatct ccgccttgcc caccctctgg gtcatctcgt agtagtcgac gccatcctct
      721 gtgcgtgcgg gccggagcac gggcggcacc ggtagaggta cctgaaaagg cctgggtagc
      781 ttcgccctgc taggcagtag ctttcccgtc gagccttcgc cggagcacgc ggccgcaccg
      841 aatgggaagg tgagcgccac gccaccagcg aggcccagcc tcaagaactc gcggcgggag
      901 atctccatac gccggatcac ccctcccgtc gcgcgaggcc ccacgaccgc ctggcgatgt
      961 agaggctagt cagcaggatc aggaacccgt tcacggggtg cagggccgca acgggtacgg
     1021 cggcgtgtgc cagcgcatat tgcgagccga tgagcaccac ggccccaagg ggcagcaacc
     1081 tcaacgccca tggcaatcgc ccgaggaagg ccacgggaac catcagcaat ggcaccatcc
     1141 caagaaactg tgcgagggtc acgtggaaag accagtccgc cccgaacgcc cccatgcccg
     1201 caaggaacac ctgtaaaagc acgcccgaag cgaggaccga agatagaaaa gcatacgaga
     1261 ccatagccag cctcgccgcc acggataccc cgactcgcgc caccttcctc gacggcttct
     1321 cgcctactcc ggcccgagcc gtatcatcca gcgtcttcac agtatggacc atcctcgcaa
     1381 cgcctcctcg ccgatcgcta gtgcttcacc ctgaagctac gagcaaacca gtcgcagagg
     1441 catcgtccga aagaaggatc caggtggttc atacgggttt atttagaatg aaggcgtcag
     1501 ccaaaacaaa aagctgatag ctgacggctg atagttgaaa gctttctccc gccaagcggg
     1561 agctaccgga tcaggtccag ctctcgcgct ctcgcgagtg cttcggtgcg gttcctggca
     1621 ccgagtttgc ggtagatgtt gcccgtgtgg ctcttgatgg tgcccacgga gacgaacagg
     1681 tccctggcaa cctccgagtt ggtcttgccc atggcgagga ggtcgagcac ctcgagctcg
     1741 cgctggctca ggggatcttc tagccgggtg gtggcctggt tggtgtcggg ccgagggagt
     1801 gcgggaccac catttggcag agctttcctg cggctttcgc cgagggaata ggccgtgccc
     1861 ccttcctccg cctgcctctc caggtggccc ttgcgggcca gctcctccag catctccgac
     1921 gcctcatcca cggtcagcga ggttcgcatc gcggcggtgg ctggggtgat ctcaccgccc
     1981 tccctcaagg tttcgagcag ttctttctct cgctctctcg cgccgggagc gggacggcgg
     2041 gacgcttcgt gcgcctctat gaggcggcct acctgcctga ggagaagcac gggggccacg
     2101 accccgatac cgaagaccgc caaccacacc acgcccacgt cggcaagccc cctgtcgatc
     2161 acgaacccca ccgaaaggag catgacaaag aggcaccata ccccgacccc gagcacggcc
     2221 agtatccgga tcagcatccg gcggccctcc cacgtattgc gttgccagga cgtcccaaac
     2281 caggctctcc ctcccgccgc cgcagcggac actggtgttg gaacacttat acgcacacgc
     2341 tcgcccgatg attcagcatc gacgaaaccc cctctgctac gggaccggcg gtaccagaac
     2401 ctccctgggc ctggagactc cccgtgtccc gagcctggac gcctgccggg cgggcaaaca
     2461 agcggaaacc cggtcatact gtgcctgggt ggacgcggga agacaggctg tacaggtggt
     2521 tcgcatgggc gagagcggag cgagaaggga ccccggtggg tggacccccc aggcgagcgg
     2581 cttgacgcca tccacgacaa cacgtacgac tggggcaccg taaaacgaag aggaaggaat
     2641 tcgatgcaca aggtaaacga gcttctcgga agagacattc tcggtcagga caccggcgag
     2701 aagcggggca cggtgaagaa cctgctcttc gacgaagcga ccacgagggt cgtagccctc
     2761 gtcgttgacg gcggcggttt cttcggcggc acgaagacgg tgcgctggga gtacgtcgtc
     2821 aacgttggcg acgtcgtgat gctggccggc ggggcgcccc tcgacaaccc gaaggaagac
     2881 cccgagatag acgccctgcg cgggcggcaa caccatatca ccggcaccaa catcgtgacc
     2941 gaagggggcg agaaagtcgg ggaggccgca gatgttctgc tcgacgacgc tggcgtcgtg
     3001 cgcgcctacg aggtcaagcg cggcgtgatc cgggacctct ccggtcgcga gcaggtgccg
     3061 gcggaggacg tcatctcctc gggcccagac gcgatcatca tccgaaacct gccagagacc
     3121 agaccataaa cccaggaggc cccggtacca gactagcggt accggggcct cctgcaacct
     3181 caggccaggg aatcccctga tgcctgtagc cttacaaacc tacctctgct cgaagtcctc
     3241 cccaggcgcg ccgcacacgg ggcactcctc gggcggctga actccctcca tctcctcacc
     3301 acaagtggtg cagaccatgg tcacggtcat gggcggatgc ggggccccgc cgtcttcggc
     3361 ctctatcttc tcacccgagt cgcgggcgag gtcggcgatc gagaccttgc cggttatgac
     3421 gtcctccatg gtggtgcgcg ctccggtgag cgagtcttcc acgagggcga gatcgatgtg
     3481 cgtcacgccg cgctcgcggg cgagcttctc ggtcatgttg cgcgagatgt tgcgcatgaa
     3541 gcccttgggg gcccggtcta gcctctcgat ggcatcgtcc gtccaggtga actccttgga
     3601 gccaccctcg gggacctgcg tgccggcctt ctggaaggcg ctgtggtcta tggggcaggt
     3661 tgcgccgctc gcctcctgga cctgttcctt ggcgtggctc actggacagc cgccgctctt
     3721 tacctcgggg gcttcgcggc cggtctcctc gcggtactgc tgggccacgt agtcgcgggt
     3781 gatcgtggtg tagcccttct tgagcgcgct cttctcgacg cgcgccttga cccggcgctt
     3841 ctcctgccac tcctcgagcg agtcgagtag ggttgtcgcg tcctcggtcc agtgcagctc
     3901 gcgtccgtcg tagacctcgg agaggttcag gttctcatcg cccgccgtgg tcgctatctc
     3961 cgcgtccacc gcctggaagt gggttggacc cgagccgcag acggggcagc gggccggctt
     4021 gcccttcgcc acgtagcggc agacgtcgca ctcgaagcgg tcctggccct gggcagcgcg
     4081 gtgtatctcc tcgcgaacct cggccgagac gccctcgata tcgccctggt cctcgctctg
     4141 tccgaacccg ccgcggcccg actcgaagat ctcctgcttg gcggccacgt cgctgctgtt
     4201 gccgttggcg ccgttggccg agccgttctt gtggccgtta gcttcgctgc cgttaccgtt
     4261 cgcgccgttg ccgttcatgc cgttgccgtt catgccgttt gccttgtcgg cggtacgctc
     4321 gtcgaagtcc ttgaacaggg agtcgatagg cttctcctcg cccatggcct cggcctcgcg
     4381 catctggtct ccgatggccc gcatggactc catggcgccg ggcgggagga tgctcttgat
     4441 cacctcgtct ataacgcccg tggttattac cgtgtagccc ttctcgatgg cgtaacggat
     4501 gatggcgccc gtggccatcc cgacca
//
LOCUS       DBNGVD010000041         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3832087, whole genome shotgun sequence.
ACCESSION   DBNGVD010000041 DBNGVD010000000
VERSION     DBNGVD010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3832087"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2665
                     /locus_tag="AC16CM_01060"
     CDS             <1..>2665
                     /locus_tag="AC16CM_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR3138445.1"
                     /translation="RLSARTRVPEEWAGLPVELELWLGGEGFVEISAGDGRTSGGLNP
                     FHRAFPMLDEALGGEPVEISAEVVSKGLFGSNVSEPRLELARLVVPQREVRALERDLT
                     AVFETCATLDDHEAVPRLLEALDEVAAVLATAWPTGTEVTLTRYLEGFAATAGHKAGS
                     LPPTYAEKLVEINRHLGEPWSLPPAPEALAPLPQRAGEAVREARGLLAARLEGIREDY
                     PPVGGIALTGHAHLDLAWLWPVAETRRKGRRTFASVLGLMDRYEDFVFNQSSAQLYRW
                     VEADDPELFERVKRRVAEGRWEPVGGSWVEPDCQIPSGESFTRQLFYGQRYFQEAFGR
                     RCTVAWFPDTFGYSPGIPQLLRGAGISGFFTYKLNWNETNDFPHDLFEWEGIDGTSIV
                     AHTFENPGTDYNGDIVPRDLYGTWRNFRGKRHHQESLFSFGWGDGGGGPSEQMLENYA
                     RLRDFPAMPRLRMAHVDAFFDSLPAQGLPRWVGELYLELHRGTLTTQARVKKLNREAE
                     HRLQEAEAFSTIAARAGASYPGEELERLWKTVLLNQFHDILPGSSISEVYQDTHRELE
                     EAVAGAERLRDRALKGLTSKDGDPARGVIVANSALRPRPFTTLLRGTVSGTVRGPDGE
                     LLPTQRTDGGMLVHAPGRSVAGLGWTSVEVRDEPSAEAPATPGARVDERDGGHVIENE
                     LLRVEIGADGSLHGVYDKEVGREVLEGRGNRLFAYADKPANWDAWDVEAGYVAEGEEV
                     AGAENVEVVESGPLRVAVRVERLFRGSRISQTYLLLSGSRRLDVETHVWWHERQVLLR
                     ALFPLRVRSHEATFETMHGVVRRPTHRNTSWDEARFEVCAHRFADLSEPGYGVALLND
                     GKYGHSARDNVLGISLLRAPLYPDPLADQGEH"
BASE COUNT          433 a          881 c          930 g          421 t
ORIGIN      
        1 cgcctgtcgg cccgcacgag ggttcccgag gaatgggctg ggctccccgt ggagctggag
       61 ctctggctcg gcggcgaggg tttcgtggag atctccgcgg gtgacgggcg gacgtcgggg
      121 ggcctgaacc ccttccaccg ggccttcccc atgctcgacg aggcgctggg cggtgagccc
      181 gtcgagatct ctgccgaggt cgtctcaaag ggcctgttcg ggtccaacgt ctcggagccg
      241 cgcctggagc tggcgcggct ggtcgtgccg cagagggagg tgagggccct ggagcgggac
      301 cttacggccg tcttcgagac ctgcgccacc ctcgacgacc acgaggcggt gccgcgcctg
      361 ctcgaagcgc tcgacgaggt cgctgctgtt cttgccacgg cctggccgac gggcacggag
      421 gtgaccctga cccgttacct cgaaggcttc gccgcaacgg ccgggcacaa ggcgggcagc
      481 ctcccgccga cctacgccga aaagctggtg gagatcaacc gtcacctcgg tgagccctgg
      541 agccttccgc cggcacctga ggcgctggcc ccacttcccc agagagccgg ggaggccgtc
      601 cgcgaggcgc ggggcttgct cgcggcccgt ctggagggta tcagggagga ctacccgccg
      661 gtcgggggga tcgcgctcac cggccacgcc cacctggacc tcgcgtggct ctggcccgtg
      721 gcggagacgc ggcgcaaggg tcggcgtacg ttcgccagcg tgcttgggct catggatcgc
      781 tacgaggatt tcgtcttcaa ccagtcatcg gcccagctgt accggtgggt cgaggccgac
      841 gaccccgagc tcttcgaacg cgtgaagcgg cgggtagccg agggaaggtg ggagcccgtc
      901 gggggatcgt gggtggagcc tgactgccag ataccctcag gcgagtcctt cacgcgccag
      961 ctcttctacg gccagagata cttccaggag gctttcggtc gccgctgcac ggtcgcctgg
     1021 ttcccagata ccttcggcta ctcgcccggc ataccgcagc tgttgcgcgg cgccggtatc
     1081 tccggcttct ttacctacaa gctcaactgg aacgagacca acgacttccc gcacgacctg
     1141 ttcgagtggg agggtataga cggcaccagc atcgtggcac acaccttcga gaaccccggg
     1201 accgactaca acggcgacat cgtcccgcgt gacctctacg gtacctggcg caacttcagg
     1261 ggcaagcgcc accatcagga gagcctgttc tccttcggct ggggcgacgg cggcgggggc
     1321 ccgagcgagc agatgctcga gaactacgcc aggcttcggg acttccccgc gatgccacgc
     1381 ctgaggatgg cccacgtgga cgccttcttc gattcgctcc ccgcgcaagg actaccacgg
     1441 tgggtcgggg aactctacct ggagctgcac cggggcaccc tcaccaccca ggccagggta
     1501 aagaagctaa accgcgaggc agagcaccgg ttgcaggagg ccgaggcctt ctccaccatc
     1561 gcggccaggg ccggcgcctc ctacccaggg gaggagttgg agcgtctctg gaagaccgtg
     1621 ctactcaatc agtttcacga catcctgccg gggagctcga tctccgaggt ctaccaggac
     1681 acccaccgcg agctcgaaga agccgtagct ggggccgaga gactgcgaga ccgggcacta
     1741 aaaggtctga cgtcaaagga tggagacccc gcccgcgggg tgatcgtggc caactcagcc
     1801 ctgcgtccga ggcccttcac caccctcttg cgggggaccg tctccggcac ggtccgcggc
     1861 cccgacggcg aactcctgcc cacgcagcgg acggatgggg gcatgctggt ccatgcccca
     1921 ggccgtagtg tcgccggact gggatggacc tcggttgagg tgcgggacga accttcggca
     1981 gaggccccgg ctacgccggg cgcgcgggtg gacgagcggg atggtggcca cgtcatcgag
     2041 aacgagctac ttcgggtgga gatcggtgcc gacggcagcc tgcatggcgt ctacgacaag
     2101 gaggtggggc gcgaggttct ggagggccgg ggtaacaggc ttttcgcgta cgcggacaag
     2161 cccgcgaact gggatgcctg ggacgtggag gccggctacg tggcggaagg ggaggaggtt
     2221 gccggagcag aaaacgtgga ggtggtggag agcgggccgt tgcgtgtggc cgtgcgcgtg
     2281 gagcggctct tccgaggctc ccggatctcg cagacctact tgttgctctc cggatctcgg
     2341 cgtctggacg tcgagacgca cgtgtggtgg cacgagcggc aggtactcct gcgggcgctt
     2401 ttcccgctgc gtgtccgttc ccacgaggcg accttcgaga ccatgcacgg cgtggtgagg
     2461 cgccccacgc accgcaacac ctcctgggac gaggctcgct tcgaggtatg cgcgcaccgc
     2521 ttcgccgacc tctccgagcc tggctacggc gtagccctcc tcaacgatgg caagtacggt
     2581 cacagcgcaa gggacaatgt tctcggcatc agcctcctcc gcgccccgct ctaccccgac
     2641 ccccttgccg accagggaga gcacc
//
LOCUS       DBNGVD010000042         5794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3650362, whole genome shotgun sequence.
ACCESSION   DBNGVD010000042 DBNGVD010000000
VERSION     DBNGVD010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3650362"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="AC16CM_01065"
     CDS             complement(<1..1129)
                     /locus_tag="AC16CM_01065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR3138446.1"
                     /translation="MLSRGNTDVEELNSQQWSKAVQDRAFVTDLQKTNENYLTVFDED
                     QTVKGLLKSDGGSDKKFEYSYTQMTDVAAQLDNQNIPYSVDPQNTGFWLTMLGTLAPI
                     LLIVLFFVLFMSSMQGGGNRVMSFGKSRARRMTKDQPKVTFSDVAGTDEAVQELTEIK
                     EFLESPQKFQKLGARIPKGALLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFV
                     GVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDG
                     FDSKSGIIMLAATNRADILDPALLRPGRFDRQIVVDRPDLPGREKILKVHTRGKPLGP
                     DVEVGTLARGTPGFTGADLANLVNEAALLAARHDKDQIDMSE"
     gene            complement(1280..1837)
                     /gene="hpt"
                     /locus_tag="AC16CM_01070"
     CDS             complement(1280..1837)
                     /gene="hpt"
                     /locus_tag="AC16CM_01070"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3138447.1"
                     /translation="MSMSSMMPDVKEVLITSQEIEDKVREIGARITRDYEGEKPLLIG
                     ILRGAVVIMSDLMRHIDLQCELDFMDISSYGTGTSSSGVVRILKDLEEDITDRHVLIV
                     EDIIDTGLTLSYLLRSLRARRPASLEICALLSKPSRRRADLDVRYLGFEVPDEFVVGY
                     GLDYAGAYRNLPDICVLKPEVFKAD"
     gene            complement(1834..3171)
                     /gene="tilS"
                     /locus_tag="AC16CM_01075"
     CDS             complement(1834..3171)
                     /gene="tilS"
                     /locus_tag="AC16CM_01075"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3138448.1"
                     /translation="MQNAGLLDVVRRTVGRHGMDLRRPLVLVSGGPDSVALLRAVVSL
                     GGDPVVLHVDHGLRREESREDADFVRGLCAGIDVACEIRRLRLRDGSNLQERAREERY
                     VLAEEVAEELGLPTIFTGHTADDVAETVLMNLARGSGLRGLSGIPPVRGRVQRPLIEA
                     TRGEVLGYLRELDQPHRTDHTNLTGKYARNRVRLEVLPVLQELYPGAARNIARAAGLA
                     REDLRVLEDLARAALERRGDEVVIGFDALRGLRPAVRRHAVRQAYAYLAPGSPPLSSG
                     LVEAVLGLQRGGEGTRTLDLPGRVVAAGREEEVVLYRRGLQPSSYGRQEIPAGGTIEF
                     GGWRISSREARFDAEDAARGDVAYLDGGKGPYAVRMAVEGDVIRPLGLGGTKKVARAM
                     MDRKVPSDLRRRTPIVVDKGGEVAWIAGGELGEAFKVGETTEKILRLEVERLS"
     gene            complement(3344..3757)
                     /locus_tag="AC16CM_01080"
     CDS             complement(3344..3757)
                     /locus_tag="AC16CM_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3138449.1"
                     /translation="MSEDSGSQGSPLKSGSGSTTISNTVVSQIAGIAAQEVEEANMGG
                     GGAVGGLLRSVAGGAGNSLSRGVTVEVGEEETAIDLSLSVAYGQSIPRISQAVRDNVI
                     KRVESLTGLRVNEVNIQVNDVQLPQEQQQLQEQQA"
     gene            4118..4666
                     /locus_tag="AC16CM_01085"
     CDS             4118..4666
                     /locus_tag="AC16CM_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3138450.1"
                     /translation="MGSRHLKVVFGATMFAGLFCLLAVAVPPEKASAATAVKTCGGES
                     LSLKAREKRTLSLHNAARTSRGLRPLCADPRLTRAARSHSREMIKKDYMAHASYNGES
                     VRERLERFGYHRSVYAENIAGGSGQPGKPGSIFGRWMDSPPHRTNILDGRFRPVGVGS
                     YTGTYRGTRNYTMYTVDFGVRG"
     gene            complement(4753..5145)
                     /locus_tag="AC16CM_01090"
     CDS             complement(4753..5145)
                     /locus_tag="AC16CM_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3138451.1"
                     /translation="MQPGSNKAVVSRFLLEGLGGLDPDVVDEVFAPDHALSSPEFGDQ
                     VITGTRIIKSAIEEFRSDSGWIRCTIQRQVEEGEWVATSYTLTESSDVHQGALFSRVV
                     AGKIVESSVVARTVATTQALMTARKAFN"
     gene            5351..>5794
                     /locus_tag="AC16CM_01095"
     CDS             5351..>5794
                     /locus_tag="AC16CM_01095"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3138452.1"
                     /translation="MSLEENKRVVRAFIEAQNEGDVAGLRELMAPDFVDHSVLPGQGP
                     SRKAYIQGVAEDNAAFSDARLIVEEQLAEGDKVMTRLRVHGHHDKANFTDLAPTGAKL
                     DTTAITINRVVDGRIAAEWSEGAGMYELTRHRLDQELKERERIDQE"
BASE COUNT         1009 a         1903 c         1819 g         1063 t
ORIGIN      
        1 tctcggacat gtcgatctgg tccttgtcgt gcctggccgc gagcagcgcg gcctcgttga
       61 cgaggtttgc caggtcggcg cccgtgaacc cgggggttcc cctggcgagc gtgccgacct
      121 ccacgtcggg gcccaacggc ttgccgcggg tgtgcacctt caggatcttc tcccgcccgg
      181 ggaggtctgg cctgtcgacc acgatctgcc ggtcgaaacg accgggcctg aggagcgcgg
      241 ggtcgaggat gtcggcgcgg ttggtggcgg ccagcatgat gatgccgctc ttggagtcga
      301 agccgtccat ctcgacgagg agctggttga gcgtctgctc ccgctcgtcg tgcccgccgc
      361 cgaggccggc gccacgctgc ctgcctacgg cgtcgatctc gtccatgaag atgatgcagg
      421 gggagttctg cttggcctgc tcgaagaggt cgcgcacgcg gctggcgccg acgcccacga
      481 acatctccac gaagtcggaa ccggagatgg agaagaaggg taccccggcc tcaccggcca
      541 ccgcacgcgc gaggagcgtc ttgcccgtac cgggaggccc gaccagcagc gcgcccttgg
      601 gtatgcgggc gccgagcttc tggaacttct gcggggattc gaggaactcc ttgatctccg
      661 tcagctcctg cacggcctcg tcggtgccgg cgacatcgga gaaggtaacc ttgggctggt
      721 ccttggtcat cctcctggcg cggctcttgc cgaagctcat gaccctgttt ccgccgccct
      781 gcatggagct catgaaaagg acgaagaaca gcacgatgag caggatgggt gcgagggtac
      841 cgagcatcgt aagccagaag cctgtgttct ggggatcgac cgagtaaggt atgttctgat
      901 tgtcgagctg ggccgcgacg tcggtcatct gggtgtagga gtactcgaac ttcttgtcgc
      961 tgccaccatc agacttcagg agccccttga cagtctggtc ctcgtcgaag accgtcaggt
     1021 agttctcgtt tgtcttctga aggtccgtga cgaaagccct gtcctgcacg gccttgctcc
     1081 attgctggga gttcagctcc tccacgtccg tgttgccacg gctcagcatg ttgacaagga
     1141 cgacggccaa gactatcagg atcacgaagt acagcagccc gccgcttctc aaaattctgc
     1201 ccaaaagctt cttaccttcc acgtaatgcg tctaggacac gagaattata ctacctgctg
     1261 gcccgatccg aggatctttc tagtccgcct tgaagacctc gggcttcagg acgcagatgt
     1321 ccgggaggtt gcggtaggca ccagcgtagt cgaggccgta cccgaccacg aactcgtcgg
     1381 gcacctcgaa gccgaggtac ctgacgtcga gatcggcgcg cctcctggaa ggcttggaga
     1441 gcagggcgca gatctcgagc gacgccggcc tgcgggcccg cagggagcgc aggaggtaag
     1501 agagcgtcag gcccgtatcg atgatgtcct ccacgatgag gacgtgcctg tccgtgatgt
     1561 cttcttcgag gtccttgagg attcgcacga cgccgcttga ggaggtgccc gtcccgtagg
     1621 agcttatgtc catgaagtcc agctcacact gcaggtctat atgtcgcatc aggtcgctca
     1681 tgatcacgac cgcgccgcgc aggatgccga tcagaagcgg tttctcgccc tcgtaatccc
     1741 gggtgatgcg cgccccgatc tcgcgcacct tgtcttcgat ctcctgcgag gttatgagca
     1801 cttctttcac gtcgggcatc atactggaca tactcatgag agtctctcca cctccaacct
     1861 cagaattttc tcggtcgtct cgcctacctt gaacgcctca ccaagctcgc cgcccgcaat
     1921 ccaggccacc tcgccgccct tgtctaccac gatgggcgtt ctgcgcctga gatcgctcgg
     1981 gaccttccta tccatcatgg ccctcgccac cttcttggta ccaccgagcc ccaacggccg
     2041 gatgacatct ccctccaccg ccatcctgac cgcgtaaggc cccttcccgc catcgagata
     2101 agccacgtcg cccctcgccg catcctcggc atcgaaacgg gcctcccgcg acgagatcct
     2161 ccaccccccg aactctatgg taccccctgc cgggatctcc tggcgcccgt agctggacgg
     2221 ctgcaagcct cttctgtaga ggaccacctc ttcctcacgt cccgcggcga ccacccgacc
     2281 gggcagatcc agggtccgcg tgccctcgcc gcctctctgg agaccaagca ccgcctcgac
     2341 caggccggag gacagcggcg gcgagccggg ggcaaggtaa gcataggcct ggcgtaccgc
     2401 gtgacgccga accgccggcc gcaaaccgcg aagcgcgtcg aagccgatga cgacctcatc
     2461 ccccctcctc tccagcgcgg ccctcgccag gtcttccagg acccgaaggt cttcgcgggc
     2521 gaggccggcg gcccgcgcta tgttgcgggc ggctcccggg tagagctcct gcaacacggg
     2581 caaaacctca aggcgcacgc ggttgcgggc gtacttgccg gtcaggttgg tgtggtccgt
     2641 gcggtggggc tgatcgagct cgcggaggta cccgagcacc tccccacgcg tcgcttctat
     2701 gaggggacgc tgcacgcgac cccgcaccgg cgggatgccg gagaggccgc gcagcccgga
     2761 gccccgcgcc aggttcatga gcacggtctc cgccacgtcg tcggccgtat gtcccgtgaa
     2821 gatggtcggg aggcctagct cctcggccac ctcctcggcg agcacgtacc tctcctcccg
     2881 cgcccgctcc tggaggttgg acccatcccg gagacggaga cggcgtatct cacaagccac
     2941 gtctatcccg gcgcagagcc cgcggacgaa gtcggcgtcc tcgcgcgatt cctcgcggcg
     3001 aagaccatgg tcaacgtgca gcacgaccgg atcgccgccg agggagacca cagcccgcag
     3061 cagcgcgacg gagtcgggtc cgccggagac gagcacgagc ggacggcgga ggtccatgcc
     3121 gtgacgcccc accgtccgcc ttaccacgtc gagcaaaccg gcgttctgca tgcgaacagt
     3181 ttagagcggt tctgagcaat tctgcaccat actcggggat agcacccggc cacgcgcgac
     3241 cgggaggcct ggtggcggaa gcgtcggggc aaccagacac agcgaaaaga ccggggcgcg
     3301 gtgccccggt ctcgcaattc tcaggccggc acggtacccg gtgtcaggcc tgctgctcct
     3361 ggagttgctg ctgctcttgc gggagctgaa catcgttgac ctggatgttc acctcgttca
     3421 cgcgcagccc ggtgaggctc tctacgcgct tgataacgtt atccctgacg gcctggctga
     3481 tcctgggtat ggactgcccg taggccacgg acagggacag gtctatggct gtctcttcct
     3541 cgcctacctc gacggttaca ccccgcgaga ggctgttgcc cgcacccccg gcaacgctcc
     3601 ggagcaggcc gccgaccgcg cctccgccgc ccatattggc ttcctcgacc tcctgggccg
     3661 cgatgccggc gatctgggat accacggtgt tgcttatggt ggtgctgccc gacccgctct
     3721 tgagggggct cccctggctg ccactatctt cgctcatgtc catcctttcc gactgctcgc
     3781 tccccaaaca ggtttctccg cgcatgacga cacaaccatc gatacacggc cgtctaagct
     3841 gcctaccccg ccgtctggga tcttaaacac gggcgacttg cgatgcaaga gccttgtgga
     3901 gggccgcagc cactggctat aagcggtcag ctctttgccc cggctagagg gttgcagcgg
     3961 ctctggttgg cggcgcgttc cgtagagcga tcggcccgtc gggctctgta ccggtgtagg
     4021 atgctctctg cacgacggct ggacgtggtg cgtcacccct acggggacct cccgaactgt
     4081 taacatgcct cgtgtcgtta ccgagggagg gggctccatg ggttcgaggc acttgaaggt
     4141 ggttttcggg gcgacgatgt ttgccgggtt gttctgcttg ctggccgtgg cggttccgcc
     4201 ggagaaggca agtgcggcca ccgccgtcaa aacctgcggc ggcgagagcc tctccctcaa
     4261 ggccagagag aagcgaaccc tctcgctaca caacgcggcc cgcacctccc gcggcctgag
     4321 acctctgtgc gccgatcccc ggcttaccag ggcggcccgt tcgcactcca gggagatgat
     4381 caaaaaagac tacatggccc atgcctccta caacggggag agcgtgcgcg aacgcctcga
     4441 gcgcttcggt taccaccgga gcgtctacgc cgagaacatc gccggtggca gcggccaacc
     4501 cgggaaacca ggctccatct tcggacgctg gatggacagc ccgccgcaca gaaccaacat
     4561 cctggacggg agattccggc cggtcggcgt gggatcctac acgggaacct acaggggcac
     4621 ccgaaactac accatgtaca cggtcgattt cggggtcagg ggctgaggtc ccccttaggt
     4681 tagcttgcgg ctggtatgag cgtccagccc ctctgggaga ggtgacgggg tattcgttgt
     4741 tggtcgagtt ggttagttga aggcttttct tgcggtcatg agggcttgcg tcgtagcgac
     4801 ggttcttgcc accacggagc tttccactat cttgccagct acgacgcggc tgaagagggc
     4861 gccctgatgc acgtcgctcg attcggtcaa cgtgtagctt gtggcgaccc actctccctc
     4921 ttcgacctgt ctctggatgg tgcatctgat ccagcccgag tcgctcctga actcctctat
     4981 ggcgctcttg atgatcctgg tgccggtgat cacctggtcg ccgaactcgg ggctgctgag
     5041 ggcgtggtct ggggcgaaga cttcgtctac gacgtcgggg tctaacccac cgagcccctc
     5101 tagaaggaac ctgctgacca ccgccttgtt gctccctggc tgcatgaacg catctccttt
     5161 ctcacctcca tgcagcatac cgtgaatcga gacgggccgc gaccacgcca tcaggcgggt
     5221 cagaaccgtg tccgtggcgc tctatggcaa gaaaagagcg cccagatggt tgcctccaac
     5281 cgcgctttgt tgcaagctga aggctgatgg gccgcgctgc gcggcgaggt ggacatcaga
     5341 aggaagctga atgtcgctcg aagagaacaa acgtgtggtc cgggccttca tcgaggcgca
     5401 gaacgaaggg gacgtggcgg ggctgcggga gctgatggcc ccggacttcg tggaccacag
     5461 cgttctaccc ggccaggggc cgtctcgtaa ggcgtacata cagggagtcg ccgaggacaa
     5521 cgccgccttc tccgacgccc ggctcatcgt cgaggagcag ttggctgagg gtgacaaggt
     5581 gatgacgcgc ctgagggtgc acggacatca cgacaaggcg aacttcacgg acctggcgcc
     5641 gaccggggcg aaattggaca ccacggccat caccatcaac cgcgtggtag acggcaggat
     5701 agccgccgag tggagcgagg gcgccgggat gtacgagctc acgcggcacc gcctcgatca
     5761 ggagttgaag gagcgggaac gaatagacca ggaa
//
LOCUS       DBNGVD010000043         4125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3832425, whole genome shotgun sequence.
ACCESSION   DBNGVD010000043 DBNGVD010000000
VERSION     DBNGVD010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3832425"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="AC16CM_01100"
     CDS             complement(<1..942)
                     /locus_tag="AC16CM_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIR3138453.1"
                     /translation="MDHAVTARADIVGSLLRPRDLLEARESYAAGGLSATEFKRVEDR
                     AVDWAIGLQERAGLPVVTDGEMRRESFQSQLVEATEGFGEHDIDAWLWGEWHGDEAVG
                     DRTTERPKTLGVVGKLRRIRHLSVEEFVYARGRTDRIVKVTLPSPSMFANLWSPELSA
                     GAYPTFDLFLEDVAQILREEVEELVRLGAEYIQIDAPHYPLLLDPKTRAFYERRGWDL
                     DRWLDRGIELDNYVIGTHPDVTFSFHLCRGNQGSRWLVSGSYEPLARRIFGGINAQRL
                     MLEYDDERSGGFEPLAHVPEDKVAVLGLVTTKTSRRET"
     gene            complement(980..1192)
                     /locus_tag="AC16CM_01105"
     CDS             complement(980..1192)
                     /locus_tag="AC16CM_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138454.1"
                     /translation="MVRVHIEAECGGAGTTRLTVRAESIRRALEIAGERNPGCALNVV
                     FPLDPDTFFVRDGAEGSELGGGEIAA"
     gene            1373..2551
                     /locus_tag="AC16CM_01110"
     CDS             1373..2551
                     /locus_tag="AC16CM_01110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3138455.1"
                     /translation="MRLARRMERLGTETAFSVLAKAKALEADGREIIHLEIGEPDFGT
                     PEHVVEAGCRALRDGHTHYTPTAGIPELREAIAQDVARSRGIEVDPTQVVVTPGGKPI
                     MFFAILALVDEGDEVLLPSPAFPIYESMVNFVGGTPIHVPLRQENGFRFDPEEFEANL
                     GERTRLVILNSPANPTGGVLTADDLGTLAGILRDHPGVYVLSDEIYSRLLYTGDFASI
                     AEHEGFGPDGRTIILDGFSKTYAMTGWRLGYGVMPRDLAEQVAKLQVNSNSCTSAATQ
                     HAGLAALRGPQDAVANMLAEFRTRRDQIVEGLNQFPGVECITPQGAFYAFPRIADTGY
                     SADELADTLLEEAGVACLSGTAFGRYGEGHLRLSYANSRENIARALDRMGELLSRTVG
                     "
     gene            complement(2635..3084)
                     /locus_tag="AC16CM_01115"
     CDS             complement(2635..3084)
                     /locus_tag="AC16CM_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3138456.1"
                     /translation="MAQGVSGPPVALEVHVTGRRVLATIVDAIIFSVYFWVLAILFGT
                     SSTEAGGISLSLGGLGTLLYLVGVFAYYLLLEGYLGQTVGKMLLGIKVVREDNGGVPG
                     LGAAALRTVLRLIDGLFLYAVAFVSVLASQKNRRLGDMVGKTLVVRK"
     gene            3289..>4125
                     /locus_tag="AC16CM_01120"
     CDS             3289..>4125
                     /locus_tag="AC16CM_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3138457.1"
                     /translation="MHRGSRKVTLADRLRYRFDNTISRGTSALVGWLALILLAMILAS
                     TLFVYLTDIAPATQGVRPGFLQTLWLGLMHTIDPGNVGGDTGGWSYLLTMLTVTAGGI
                     LVFSALIGVIFSGIDQKLANLRKGRSFVVEHGHTVIYGWSPEVFPVVSELARANESRG
                     RSCIAILAEEDKVEMDDQLRARVGKTGRTRVVCRTGDPINIEDIAIVNPDEARSIIVL
                     PTEDGEADSRVIKTVLALTNNPDRPREPSHIVSRIGRPENLGVARMVGRDELRLVAVD
                     DLI"
BASE COUNT          746 a         1389 c         1278 g          712 t
ORIGIN      
        1 cgtctcccgg cgggacgtct tggtcgtaac caggccaagc acggccacct tgtcctcggg
       61 cacgtgcgcg aggggttcga agcctcccga gcgctcgtcg tcgtactcca gcatcagcct
      121 ctgcgcgttg atgccgccga agatgcggcg ggccagtggc tcgtaggatc cggagaccaa
      181 ccagcggctg ccctggttgc cccggcagag gtgaaaggag aacgttacgt ccgggtgggt
      241 cccgatcacg tagttgtcca gctcgatgcc ccggtccaac cagcggtcca gatcccagcc
      301 ccgcctctcg tagaaagcgc gggtcttcgg gtccaggagc aaggggtagt gtggcgcgtc
      361 gatctggatg tactcggcgc caaggcgcac cagttcctcg acctcctcgc gcaatatctg
      421 cgccacgtct tccaggaaga ggtcgaacgt gggataggcc ccggcagaca gctccggcga
      481 ccagaggttg gcgaacatgc tgggactcgg caacgttacc tttactatcc tgtcggtacg
      541 gcccctggcg tacacgaact cttccacgga gagatggcgg atgcgccgca gcttaccgac
      601 gaccccgagg gtcttgggac gctcggtcgt cctgtcccct accgcctcat caccgtgcca
      661 ctcgccccac agccaggcgt cgatgtcgtg ctcgccgaag ccctctgtgg cctcgaccag
      721 ctggctctgg aaggactccc ggcgcatctc accgtccgtg acgacgggga ggccggccct
      781 ctcctgcagc ccgatggccc agtccacggc cctgtcctcc acgcgcttga actcggtcgc
      841 ggagagtcca ccagcggcgt agctttcgcg cgcctcgagt aggtcccggg gcctcagcag
      901 gcttccgacg atatccgcgc gggctgtaac ggcatgatcc acgcggaaca gtctagcacc
      961 agatctcgtg ccggtaagcc taggccgcta tctcgccacc cccgagctcc gagccctctg
     1021 ccccgtcccg gacgaagaag gtatccgggt cgagggggaa aaccacgttc agcgcgcagc
     1081 cggggttccg ctctcccgct atctccagcg cccgccggat gctctcggcc ctcacggtca
     1141 acctggttgt gccagccccg ccgcactccg cctcaatgtg cactctaacc attaccgggc
     1201 tcctccatcc tctagctttc ccttctcatc ctttgtacgc tcctgccatt acatatcggt
     1261 tacgggtcgg caaaatgtag gcaacaggta ttacgaacgc tgcgtggggt tggatcaggc
     1321 gcgcctgtgg tgtaattcgg ggcaacgaac gtagatctgg gtggaggtgc cgttgagact
     1381 ggcgaggcgt atggagcgac ttggaacgga gacggccttc tccgtcctgg cgaaggcgaa
     1441 ggctctggag gccgatggac gcgagatcat ccacctagag atcggcgagc ccgacttcgg
     1501 tacgccggag cacgtggtcg aggccggatg ccgagccctg cgcgacgggc acacgcacta
     1561 cacccccacc gcgggcatcc ccgagcttcg agaggccatc gcgcaggacg tagcccgcag
     1621 ccgcggcatt gaagtagacc ccacccaggt ggtcgtcacg ccaggcggga agccgataat
     1681 gttcttcgcc atcctggcgc tggtggacga gggtgacgag gtcctgttgc ccagcccagc
     1741 gttcccaatc tacgagtcga tggtcaactt cgtcggcggg acaccgatcc acgtgccgct
     1801 gcgccaggag aacgggttcc gcttcgaccc ggaggagttc gaggcaaacc tgggcgaacg
     1861 gaccaggctc gtgatcctca actcgcccgc caacccgacc ggcggcgtgc tcacggccga
     1921 cgacctgggc acgctcgccg gcatcctacg tgatcacccc ggcgtgtacg tgcttagcga
     1981 tgagatctac tcccgactcc tctacacggg tgacttcgcc agcatcgccg agcacgaggg
     2041 ttttggcccc gacgggcgca ccatcatact ggacggcttc tccaagacct acgcgatgac
     2101 gggctggcgt ctcggatacg gcgtcatgcc cagggacctc gcggagcagg tcgccaagct
     2161 gcaggtcaac tccaactcct gcaccagcgc agccacccag cacgccggcc tcgcggccct
     2221 gcgtgggccc caggacgccg tggcgaatat gctggccgag ttccggacgc ggagagacca
     2281 gatcgtggaa ggcctcaacc agttccccgg cgtagagtgc atcacgcccc aaggcgcgtt
     2341 ctacgcgttc ccgcgcatcg cggacacggg ctattcagcc gacgagctcg cggacacgct
     2401 cctggaggag gccggcgtgg cctgcctctc cggcacggcg ttcggccgct acggggaagg
     2461 ccatctccgc ctctcgtacg ccaactcccg ggagaacatc gccagggcgc ttgaccgcat
     2521 gggggagtta ctctcgagaa cggtcgggta gcacctacaa gtcgctcgac ccgggcaagg
     2581 ggacgggccg ggatctcatg ccagatcccg gcccgtctgg ttctggtggt ctctctactt
     2641 gcgcactacg agggtcttgc cgaccatgtc accgaggcgc cggttcttct ggctggcgag
     2701 gacggatacg aaggccacag cgtacaggaa caggccgtcg atgaggcgca acacggttct
     2761 cagggcggcg gccccgagcc ccggcacccc accgttatcc tctcttacca ccttgattcc
     2821 gaggagcatc ttgcccacgg tctggccaag gtagccctct agcaacaggt aataggcgaa
     2881 taccccgacg aggtagagga gcgttccaag gcccccgagc gagagggaga ttcccccggc
     2941 ctccgtcgag ctggtcccga agagaatggc caacacccag aagtacacgg agaagattat
     3001 ggcgtccacg atggtcgcca gcactctcct gcctgttacg tggacctcta atgcgacggg
     3061 aggtccggat acaccctgag ccataacgtt tctcctctct gccacccgat gacaaaccga
     3121 caagacttat catgcacgca tccggcggat ggcgcatcct ccaaatggac tatcttttgt
     3181 cccgggcata acgaaataca tcagctcgtt catacattgc gtggcccccg caacgtcgga
     3241 catcagaacg gcgatataat ctcgctccga cgtctacccg ggggttctgt gcacaggggt
     3301 tcgcggaagg tcacgctcgc cgaccggtta cgataccggt tcgacaacac catctccagg
     3361 ggcacgagcg ccctggtagg ctggctagcc ctgatcctgc tggccatgat cctggcgagc
     3421 acgctcttcg tctacctgac cgacatcgcg cctgcgaccc agggcgtacg gcccggcttc
     3481 ttacagacgc tctggctcgg cctcatgcac accatagacc ccggcaacgt cggcggcgac
     3541 acgggtggct ggtcctacct gctcaccatg ctgaccgtca ccgccggcgg catcctggtc
     3601 ttcagcgcgc tcatcggcgt gatcttctcc ggcatcgacc agaaactggc caacctgcgc
     3661 aagggccggt ctttcgtggt ggagcatggg cacacggtca tctacggctg gtcgcccgag
     3721 gtttttcctg tggtgtccga gttggcgagg gccaacgaga gccgggggcg gtcgtgcatc
     3781 gccatcctgg ccgaggaaga taaggtcgag atggatgatc agctccgggc cagggtaggc
     3841 aagaccggca ggacgcgggt cgtatgcagg acgggtgacc ccatcaacat agaggacatc
     3901 gcgatcgtca atccggacga ggcacgttct atcatcgtgc ttcctaccga ggacggagag
     3961 gccgactcgc gggtgatcaa gaccgtgctg gcgctaacca acaacccgga ccggcccagg
     4021 gagccctccc acatcgtgtc gaggatcggc cgccccgaga acctcggggt ggcgcggatg
     4081 gtcgggcgcg acgagctgag gctcgtcgcc gtcgacgacc tgatc
//
LOCUS       DBNGVD010000044        10520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3286144, whole genome shotgun sequence.
ACCESSION   DBNGVD010000044 DBNGVD010000000
VERSION     DBNGVD010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3286144"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="AC16CM_01125"
     CDS             <1..500
                     /locus_tag="AC16CM_01125"
                     /inference="COORDINATES: protein motif:HMM:NF042664.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3138458.1"
                     /translation="QPQQQNQTPGPEQQIAAAEKELKQDPNDPENIKDLAYLYYTAGR
                     YDDAIQVLQDGQKAAPKDEEIPALLGQIYSQQAQTASGKKQEELQIKAAEAFAASAEA
                     QPDNAEAYLLAGQAYEQAGQPAEAIQYYNQYLDRAPNGQEANQVKERISALLEGKETT
                     GSAQP"
     gene            566..838
                     /locus_tag="AC16CM_01130"
     CDS             566..838
                     /locus_tag="AC16CM_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011115550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3138459.1"
                     /translation="MFGSIGMPELIIGLVVILLLFGAKRVPELARGLGSGVREFKKGQ
                     QEGQVEKKEEEQEDEQLEASKNGHDDEARLEDDSPAEKAEQQQHSS"
     gene            919..1722
                     /gene="tatC"
                     /locus_tag="AC16CM_01135"
     CDS             919..1722
                     /gene="tatC"
                     /locus_tag="AC16CM_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3138460.1"
                     /translation="MTLIEHLDELRSRIIKVGIAFLAVSIVAWFFRQRVFDLLLAPAG
                     DALEGKLNVTGVTEQLFTDMKLALYVGFLFTIPILLYQLWAFIAPAVGDMGRVFTYTI
                     ITLASVLFLAGVAFGYFVVLPIGTQFLLNWDTARFDPIITAQSYLPFVTRFLLAFGIV
                     FELPAATYVAAKLELVDAPLLKRYRKHAIIINTVLAAALTPGQDPYSMILMAVPMIVM
                     YEISIIIARYVNPTTEVTAHELARNEEDEDRDLDEAPDDHPENGHERDL"
     gene            complement(1765..2238)
                     /locus_tag="AC16CM_01140"
     CDS             complement(1765..2238)
                     /locus_tag="AC16CM_01140"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3138461.1"
                     /translation="MAEKPRPFRASYLISGVAPEEIFDALLDVPRFPEWAAGLKRARV
                     VDAAGGETSDLRPGTMLEFTLSAASITHQVASTVTVVEPSQRLEWNYVEGATGSGGWL
                     IEDGGTGAVRMTLWTDYRIHPGWLDKIAHRPFFRRLTEDLIRRSLRRFDAHLREG"
     gene            complement(2231..3133)
                     /gene="menA"
                     /locus_tag="AC16CM_01145"
     CDS             complement(2231..3133)
                     /gene="menA"
                     /locus_tag="AC16CM_01145"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HIR3138462.1"
                     /translation="MTSSPRRQVFREWFILARPFSLTASAVPVLFGTALAYKDGAFAW
                     GPLLAMLLGSLLIQAATNMFNEFYDEQRGLDVEGAVGIAGSIVSGRVSARGVLAGALL
                     CYTAALFLGIYLISVGGWPILVLGCLSALGGYLYSAGPRPIAYTPASEAAVFVFMGVL
                     IVVIAYAVQAREFPLYVPLAALPIGGPVAAILLANNIRDLVSDRRGGRRTLPIVFGRE
                     TAILVYRLLLLEAYVAVAVLMAFRIVPPECALVFPSVFACFRLWRDIAAYSTPARLDP
                     VVKRTAALHLVFGLLYTVGVLIGG"
     gene            3292..4197
                     /locus_tag="AC16CM_01150"
     CDS             3292..4197
                     /locus_tag="AC16CM_01150"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR3138463.1"
                     /translation="MEADRSRGLLRSYLRLVRPKQWTKNGFVLAGVVFAEKAFSVTAV
                     THALLAFVVFCALSGAVYAANDVLDVEEDRKHPLKRLRPVASGEVSPRAALFFAGMLA
                     IGALTVSFVFLPFGVGVAGIAYLVLQVLYTTVFKHTAILDVMSISAGFVIRALAGVAA
                     VSSPISPWLIVCTGLLTLFLGFSKRRHEIATLGEGAVVHRRNLQDYSVPLLDEMMNIM
                     ISATIIAYTLYTFTVYEREDQIFMMGSIPFVVYGVFRYMLLVHRDGGGNPDTLLLSDR
                     PLQIALLLWLVVVMGVIYVVPFWNA"
     gene            4185..5183
                     /locus_tag="AC16CM_01155"
     CDS             4185..5183
                     /locus_tag="AC16CM_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3138464.1"
                     /translation="MERLRRNLVLALALGVAVYVILAVFSNFDDLLSALDSFNYALIP
                     AILGLVTLSYVGRFFRWVYYLKVLKVSVPTGMNAAIFASGLSMAISPGKLGEVLKSVF
                     IRRTSGVPIARTAPAVVAERATDGTGMVIWGFLGAFALALPPWTMLVFLAVAAFGIAV
                     LRSKRLSLAVERVLVKLPLVNRFAPHLNDFHGASNALLATGPLLIGTVISFLSWGLEC
                     TGVYLCAVGLGVDKPFLLIVFVFATSSLLGVVSMLPGGIGAVEAGLYGQFVTVANLST
                     GVAGALTLLIRLATLWFATLVGIGGLILVQRLLGDASLEAEPSAVSDQQSARKADG"
     gene            complement(5222..5986)
                     /locus_tag="AC16CM_01160"
     CDS             complement(5222..5986)
                     /locus_tag="AC16CM_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR3138465.1"
                     /translation="MSEWFGTGRGRLGLYALGVIAVVAIGVLGVMAGTGWLTGAGADP
                     AKRAIQPVGADKEMPEPIARGAAKMADDAEPEKKTDQAERAVKKSEPKPDPKPEPKKK
                     VNAPAAVPADTYMTLTVPKMGITDIPVVEGTTEASLSQGAGHYTGSGYPWVAGSNTYI
                     AGHRIGYPGTASDHVFWDLPSLAMGDKIYLTDTNGHTYTYAVSDILEVPVDDLSVTAP
                     TGTDTVSLQTCIENYGDYWTPGPNWYVRYVVRGTPV"
     gene            complement(6175..7416)
                     /locus_tag="AC16CM_01165"
     CDS             complement(6175..7416)
                     /locus_tag="AC16CM_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138466.1"
                     /translation="MLRRLPALELWEPLKGWSFRRLWAASGLSMLADQAFLVALTWLV
                     LRLTGSGAELGAVLAVAAIPGAVLMPLGGVLSDRASPVTIMIGASVGRVVLLALLAVL
                     ILTGEIRLWHVYVISASLSALDALYYPASMAILPALVGRDRLGAANAMIQGVEQASSI
                     LGPALAGLLLALAGLGASFGGTALLFLVATALLVTLARAANQVSGPEDDQEDEQNTTG
                     GGLAEGLRYVVGDPVIRTLLLVVLGTNLAMMGPLYVGGAVLAESRLGGAGAFGTLVAA
                     AGAGSLIGIVGAGSVRRFARRGLIELSLTGILGIVVASIAFVPNLALAVPIAIAIGAT
                     ASFLGVVTISWLQERTEPGLLGRVMSLVMFSAVALDPISFALAGVLVEVNLAATFVGA
                     GSLLVLTAPLGATSRTMRLAS"
     gene            7548..8204
                     /locus_tag="AC16CM_01170"
     CDS             7548..8204
                     /locus_tag="AC16CM_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138467.1"
                     /translation="MQIAQKFERLLETYRHPDGRRWSGQEIDEATGGVVTRSYVTNLR
                     KGRIENPGFEKLRAIAKVMGFSPELWFEQASDLGTGASSQYLSREGSIADRTNRLFDV
                     VRNEKSGLPFTNAEVARMSLGDLKEEHVEQIRAGTLTNPSMNQVVALAEVFGVHPSYF
                     LDATRKPPLLDEDAMRILQDETVSAIAHKSLNLPGRERQMILNIIQQFEDTQEAGGGG
                     "
     gene            8194..8841
                     /locus_tag="AC16CM_01175"
     CDS             8194..8841
                     /locus_tag="AC16CM_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138468.1"
                     /translation="MAAEAPGPGLVTAVRAEVCSLWNHLRYGTGARAHGRRPLLTAAE
                     FQEELAVLGLSGRSFVPKDYADALGDYLGISINIHVIPDDWYPELSRRLAISGRLGEL
                     RYSEQLTLAAIFVPGSLPPLVSTLTILHELGHLAAGDVLIVPSQESGGPQDQPAGGST
                     YITTSVRRGKKLARGLPFAEESLREREANMRASYALFAGCLGSDSPYADAMFSVL"
     gene            8838..10304
                     /locus_tag="AC16CM_01180"
     CDS             8838..10304
                     /locus_tag="AC16CM_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138469.1"
                     /translation="MSAVQHQALVLGALSFVAFAFFACVALSSRRTRFSVFAACNLLF
                     ATLVFCGVAAFDLSHERTEVVVALMTELPVVLIVAWVCVALAGRLPHLSGTLPLEPAA
                     RSAGLRKLLRSAPFALLFFVGVACVVGMVFPSPAIRVYATAPIEFFVMKSLIMVPEAF
                     YAGLAALVFGVMAGRSSIPKRRLFYKTLAFSSGMAVIALIALVSIVFAGVRVWVSDQN
                     RTAILKMLNTFEACLAISCIAVFALGLSLRYTPAVAGPLLQRLQTGWLHAQEQFESLE
                     WRAVSTGAAQRLRDASGTVALACRFQNVPEPEIEKALATIRLVAVMKDPSSETQHITP
                     EAARKLYELQEEILGDDVLASRISWAAGLRSRAREPQTVKSAPLHDAIRAALDLIDDQ
                     DNEVRMETRPPWYWIAAVSAADAKIIDPPTAESLRSAEHEGYRAAADAYHAAKSSRQT
                     LLGDYPERRSTYDKPRTKATESRGGEGLTVEPASRGEE"
     gene            10307..>10520
                     /locus_tag="AC16CM_01185"
     CDS             10307..>10520
                     /locus_tag="AC16CM_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138470.1"
                     /translation="MKNEDTRSQQTGSHGGPSGRSGLLAMVRNRTESEHDASSSATDL
                     TLSAAGLGQAPYVAVGSGIVVVQRNCH"
BASE COUNT         1924 a         3413 c         3290 g         1893 t
ORIGIN      
        1 accagcccca gcagcagaac cagacccccg gtcccgagca gcagatagca gccgccgaga
       61 aggagctgaa gcaggacccc aatgacccgg agaacataaa agacctcgcc tacctgtact
      121 acacggcagg ccgctatgac gacgcgatcc aggtgctgca ggatgggcag aaggcagccc
      181 ccaaagacga ggagataccc gcgcttctgg gccagatcta cagccagcag gcgcagaccg
      241 cctccggcaa gaagcaagaa gagcttcaga taaaggcggc cgaagccttc gccgcctcag
      301 ccgaggcgca gcccgacaac gcggaggctt accttctggc gggccaggct tacgagcagg
      361 ccggacagcc cgcagaggcc atccagtact acaatcagta cctcgatcgc gcccccaacg
      421 gccaggaggc gaaccaggtc aaggagagga tctccgcgct gctcgaaggc aaggagacca
      481 ccggcagcgc gcagccctga tccagcttcc gaccgaagtc gtataatgtt cgtgagatcc
      541 ggcagatagc ccgttaggag gaaaggtgtt tggttccatc ggaatgccag agttgatcat
      601 cggcctggtc gtgatcctgc tactcttcgg cgcgaagcgt gtccccgagt tggcccgcgg
      661 cctcggaagc ggtgtccgcg agttcaagaa gggtcagcag gagggtcagg tcgagaagaa
      721 ggaagaggag caggaggacg agcagctcga ggcctccaag aacggacacg acgacgaggc
      781 caggctggag gacgactcgc ccgctgagaa ggccgaacag cagcagcata gtagctagac
      841 tcacctagat ctaagctccg gatggcaggt cgcctcagac taccctcgcg tccgcgcggc
      901 ccgcgcgacg aggctcgcat gaccctgatc gagcacctcg acgagctgcg ttcgaggatc
      961 atcaaggtcg ggatcgcgtt cctcgccgtt tcgatcgtcg cctggttttt caggcaacgc
     1021 gtcttcgatc tgctgctggc gccggcgggc gatgcgcttg aaggcaagct gaacgttacc
     1081 ggtgtaacgg agcagctctt caccgacatg aagctggccc tctacgtagg gttcctgttc
     1141 accatcccga tccttctcta ccagttgtgg gcgtttatag cccccgccgt cggagatatg
     1201 ggccgcgtct tcacctacac catcataacc ctggcctccg tgctgttcct ggccggggtg
     1261 gccttcgggt acttcgtcgt gttgccgatc ggtacgcagt tcctgctgaa ctgggacacc
     1321 gcccgatttg atcccataat caccgcacag agctacctgc ctttcgtgac gaggttcctg
     1381 ctggccttcg gaatagtctt cgagctgcct gcggccacct atgtggccgc aaagctggag
     1441 ctcgtcgatg ccccgctgct caagcggtac aggaagcacg ccataatcat aaacaccgtt
     1501 ctcgccgccg ccctcacgcc gggccaggac ccctacagca tgatcctgat ggccgttcca
     1561 atgatcgtga tgtacgagat ctctatcatc atcgcccgct acgtgaaccc gaccacggag
     1621 gtcacggcgc acgagctggc aaggaatgaa gaggacgagg accgagacct cgacgaggct
     1681 cctgacgacc accccgaaaa cggccacgaa cgagatctgt aggggctggc ttcgaaccag
     1741 cccgggaacg acccgccctg actcttaacc ttctctgagg tgcgcgtcga agcgccgcag
     1801 ggaacgccgg atcaggtcct ctgtcaggcg gcggaagaag ggcctgtggg cgatcttgtc
     1861 gagccagccc gggtggatcc tgtagtccgt ccagagcgtc atcctgacgg cccccgtgcc
     1921 cccgtcctct atgagccagc cgccgcttcc cgtggcgcct tcgacgtagt tccactccag
     1981 acgctgtgat ggctcgacca ccgtgacggt gctggccacc tggtgggtga tgctcgccgc
     2041 gctgagggtg aactcaagca tcgtccctgg gcgcaggtcg gaggtctcgc ctccagcagc
     2101 gtcgacgacc ctggcgcgct tgaggccggc ggcccactcg gggaagcggg gtacgtcgag
     2161 gagggcatcg aagatttcct cgggcgctac cccggagatg aggtagctgg ccctgaaggg
     2221 cctgggcttc tcagccaccg atcaggaccc ccaccgtgta gagcaacccg aagacgaggt
     2281 ggagggcggc cgtgcgcttc acaacggggt cgaggcgggc gggtgttgag tacgcggcga
     2341 tgtcccgcca gagccggaaa caggcgaaca cgctggggaa gacgagcgcg cactcgggcg
     2401 gcactatacg gaaggccatg agaacggcga cggccacgta ggcctcgagc aagagcagcc
     2461 ggtagacgag gatggccgtc tccctaccga agactatggg cagcgtgcga cgcccgcccc
     2521 tgcggtcgga gacgaggtcc ctgatgttgt tggcgagcag tatagcggcc accggtcccc
     2581 cgattggaag cgcggcgagc ggcacgtaca gggggaattc cctggcctgc acggcgtagg
     2641 cgatcaccac gatcaagacc cccataaaca cgaagaccgc cgcctcgctg gccggtgtgt
     2701 aggctatggg acgcggccct gccgagtaga gatagcctcc tagcgccgac aggcacccga
     2761 gcacgagtat gggccagccg cccacgctta tgaggtagat cccgaggaag agcgcggcgg
     2821 tgtagcagag tagggccccc gccaggaccc cgcgcgcgct cacgcgcccg ctcacgatgg
     2881 accccgctat ccccacggcc ccctcgacgt cgaggccacg ctgctcgtcg tagaactcat
     2941 tgaacatgtt ggtggcggcc tgaatgagca agctccccaa caacatggcg agcaggggtc
     3001 cccaggcgaa cgcgccgtcc ttgtaagcca gcgccgtccc gaacagcacg ggcaccgccg
     3061 aagccgtcag gctgaagggc ctggcgagga taaaccattc ccggaaaacc tgccgtctgg
     3121 gtgaggatgt cattcgggca attgtggaga gggtcggccg cttttgcaat cgccccgttt
     3181 acggttttca agctttcggc tgtcagcgat cagcggtcag cctcttgttc ttgctgaaag
     3241 ctgatggcta aaagctgaaa gctgacagct actatgatac aaccggcagt cgtggaagcc
     3301 gatcgttcca ggggactctt gagatcctat ctgcggctgg tccggcccaa gcagtggacc
     3361 aagaacgggt tcgtgctggc cggggtggtg ttcgcggaga aggctttctc ggttacggcc
     3421 gtaacacacg ccttgctggc cttcgtggtt ttctgcgctc tctctggtgc cgtctacgcc
     3481 gccaacgacg tgctcgacgt cgaggaggac cgcaagcacc cgctcaagcg gctaaggccc
     3541 gtggcgagcg gtgaggtctc gccccgcgcc gccctttttt tcgccggcat gctggccatc
     3601 ggcgcgctca cggtcagctt cgtgttcctc ccgttcggcg tgggggtggc cggcatcgcc
     3661 tacctcgtct tgcaggtcct gtacaccacc gtcttcaagc atacggccat cctggatgtc
     3721 atgagcatct ccgccgggtt cgtcatcagg gcgctcgcgg gtgtcgccgc ggtgagcagc
     3781 ccgatctcgc cctggctcat cgtctgtacg gggctcctta cgctctttct cggcttctca
     3841 aagcgcaggc acgagattgc gaccctcggt gagggtgctg tggtccacag gaggaatttg
     3901 caggactact ccgtgccgct gcttgatgag atgatgaaca tcatgatctc ggccaccatc
     3961 atcgcctaca cgctctatac cttcacggtc tacgagaggg aggaccagat cttcatgatg
     4021 gggagcatcc cgttcgtggt ctacggtgtt ttccgctaca tgctcctcgt tcaccgcgac
     4081 ggcggcggca accccgatac ccttctcctc tccgacaggc cgctccagat agccctcctg
     4141 ctctggctgg tcgttgtgat gggcgtgatc tacgtggtgc ccttttggaa cgcctgagga
     4201 ggaacctggt actggccctc gcccttggcg tggccgtgta cgtgatcctg gccgtcttct
     4261 ccaacttcga cgacctccta tccgccctgg acagcttcaa ctatgccctg ataccggcga
     4321 tactgggcct cgtcaccctc tcttacgtgg gccgcttttt ccgctgggtg tactatctga
     4381 aggtgctgaa agtctccgtt ccaaccggga tgaacgccgc catcttcgcc tcggggctct
     4441 ccatggcgat ctcaccaggc aagctgggag aggtgctgaa gagcgtcttc atccgcagga
     4501 cctcaggcgt cccgatagcc cggacggccc ccgcggtggt ggccgaacgc gccacggacg
     4561 gcacgggcat ggtcatctgg ggtttcctcg gcgccttcgc cctggccctg ccgccgtgga
     4621 cgatgctggt gttcctggcc gtcgcggcct tcggcatcgc ggttcttcgc tccaagcggc
     4681 tctcgctcgc cgtggagcgc gtgctggtga agctgccgct cgtgaaccgc ttcgccccgc
     4741 acctgaacga ctttcacggt gcctcgaacg ccctgcttgc gacgggaccg ctactgatag
     4801 gaacggtcat ctcatttctg tcctgggggc tggagtgcac gggcgtctac ctgtgcgccg
     4861 tcgggctcgg cgtggataaa cccttcctgc tcatcgtttt cgtcttcgcc accagctcgc
     4921 tgctaggcgt cgtcagcatg ctgccagggg gaataggtgc ggtcgaggcc ggactctacg
     4981 gccagttcgt caccgtcgcg aacctctcca cgggtgtcgc tggcgcgctc accctgctca
     5041 tacgcctcgc cacgctctgg ttcgcaacgc tggttggcat cggcggcctg atacttgttc
     5101 aacgcctcct cggcgacgca tcgctggaag cagagccgtc agctgtcagc gatcagcaat
     5161 cagccagaaa agctgacggc tgaaagctaa aggctgaaag cgcggttcgc aaggcgagcc
     5221 gctagaccgg cgttcccctg acgacgtaac ggacgtacca gttcggacct ggcgtccagt
     5281 aatctccgta gttctcgata caggtctgga gcgagaccgt gtcggtgccc gttggggccg
     5341 tcaccgagag gtcgtctact ggaacctcga ggatgtcgct gacggcgtag gtgtaggtgt
     5401 gtccgttggt gtccgtgagg tagatcttgt cccccatggc caggcttggc aggtcccaga
     5461 agacgtggtc gctcgcggtg ccgggatacc cgattctgtg ccccgcgatg tacgtgttcg
     5521 agccggcgac ccaggggtag ccagagccgg tatagtgccc agccccctgc gagagactgg
     5581 cctcggtggt gccctctacc acagggatgt cggtgatgcc catcttcggt accgtcaggg
     5641 tcatgtaggt gtcggccgga acggccgccg gcgcattcac cttcttcttc ggctccggct
     5701 tcgggtccgg cttcggctcc gacttcttga cggcgcgctc tgcctgatcc gtcttcttct
     5761 cgggttcagc gtcgtcggcc atcttggccg cccccctggc gatcggctcg ggcatttcct
     5821 tgtccgcacc aacgggctgg atagccctct tcgccgggtc cgcgcccgcc ccggtgagcc
     5881 atccggtccc ggccattacc ccaagcaccc cgatggcgac caccgctatg acaccaaggg
     5941 cgtataaccc aagcctcccc cggccggtcc caaaccattc actcatcgcg tctccccaaa
     6001 ctacctcccg aaaggggggt tcctgtgctc tcttcggcca gacccgggaa cccggttcat
     6061 cgcctgtgat gcgtcgcact ttaacactgc catacaagaa ccgcaacaaa cttaacaaat
     6121 ttgtaacggc ttgatcggga gggaataagg cgggctagtt ggctggaggt ctgctcagct
     6181 tgccaggcgc atcgtgcggc tcgtagcacc caacggggcc gtcagaacca gaagcgatcc
     6241 cgcgcccacg aatgtcgccg ccaggttcac ttcgaccagt acgccggcca gggcgaacga
     6301 gatgggatcc agggccaccg cagagaacat cacgaggctc atcacgcgcc cgagcaggcc
     6361 cggctcggtg cgttcctgaa gccaggagat cgttactacc ccgagaaagc tcgcggtcgc
     6421 gcctattgct atggcgatag ggacagcgag ggccaggttg ggcacgaagg cgatcgacgc
     6481 gacgacgatg ccgagaatcc ctgtcaggga taactctatg agcccccgtc gcgcgaaacg
     6541 gcggaccgat cccgcgccca cgatcccgat cagggacccg gcccccgcgg ctgccaccag
     6601 ggtgccgaag gccccggcgc ccccgaggcg ggattcggcg agcacggccc ctcccacgta
     6661 aaggggaccc atcatggcca ggttcgtacc gagcacgacc agcagcagcg tgcgtatgac
     6721 ggggtctccc accacgtagc gtagtccttc ggccagccca cctcccgtgg tgttctgctc
     6781 atcctcctgg tcatcctccg gtcctgatac ctgattcgcg gcgcgcgcca gcgtgacgag
     6841 gagcgccgtc gcgaccagaa agagaagggc tgtgccgccg aagcttgccc cgaggccggc
     6901 gagcgcgagc aacagcccgg ccaatgcagg tccgaggatg ctgctcgcct gctcgacgcc
     6961 ttggatcatg gcgttcgccg cgccaagccg gtcccggccg accagggccg gtaagatggc
     7021 catcgaggcc ggatagtaca gcgcgtcgag cgcgctaagg ctggccgaga tcacgtacac
     7081 gtgccagagc cgtatctcgc ccgtaaggat caggactgcg agcagggcca agagcaccac
     7141 gcgccccacg ctggcgccta tcatgatcgt cacgggcgag gcccggtcgc tcagaacacc
     7201 gccaaggggc atgaggacgg cgcctggtat cgcggcgacc gcgagcacgg cgcccagctc
     7261 cgcgccagac cccgtcaaac gcagaacaag ccacgtcagg gccaccagaa acgcctggtc
     7321 tgccagcatc gaaagccccg acgcggccca caaccgccgg aaggaccacc ctttcaaagg
     7381 ctcccagagc tccagcgccg gcaaccgcct cagcatggcg taaggacccc gcagatcctg
     7441 gcggcatctc tggtgacgaa ttttacttcg ttgcattgat tatacatttc gagttatggt
     7501 atcgtatatt caacaaaggt actacggggt ggaggcagag ggtccggatg cagatcgcgc
     7561 agaagttcga gcgcttgttg gagacgtatc gtcatccgga cggacgcagg tggagcggcc
     7621 aggagatcga cgaggcgacg ggtggtgtcg ttacccgttc ctacgtcacc aacctcagga
     7681 aaggacgaat agagaaccct ggcttcgaga agttgagggc catagccaag gtcatgggct
     7741 tctcgccgga actgtggttc gagcaggcat cggatctcgg cacgggggcg tcgtcgcagt
     7801 acctgagccg ggagggcagc atcgccgaca gaacgaaccg actgttcgac gtggtcagga
     7861 acgagaagtc gggcttgcct ttcaccaacg ccgaggtagc gcgcatgagc cttggcgacc
     7921 tcaaggagga gcacgtcgag cagatcaggg cgggtaccct caccaacccc tccatgaacc
     7981 aggtggtcgc cctcgccgag gtcttcggcg tgcatccctc gtactttctc gacgcgacca
     8041 ggaagccgcc cctgctggat gaagatgcga tgcgtattct ccaggacgag acggtcagcg
     8101 cgatcgccca caagagcctg aacctgccag gacgcgagcg gcagatgatc ctcaacatca
     8161 tccagcagtt cgaagacaca caggaggccg gtggtggcgg ctgaagcccc ggggcccggc
     8221 ctcgtaacgg cggtacgcgc ggaggtctgc tcgctctgga accatctccg gtatggcacc
     8281 ggcgcccggg ctcatggacg gaggccgttg ctcacggccg ctgagtttca ggaagaattg
     8341 gccgtcctgg ggttgtcggg caggagtttc gtgcccaagg attacgccga tgccctcggc
     8401 gattatctgg gcatatctat caacatccac gtaatccccg acgactggta cccggagctc
     8461 tcgcgccggc tagcgatctc gggacgactt ggagagctac gatattcgga gcagttgacg
     8521 ctggccgcaa ttttcgtgcc ggggagccta cctccgcttg tctcgaccct gactatcctt
     8581 catgagctcg gacacctggc cgcgggtgac gtcctgatcg tgccctccca ggaatcgggt
     8641 gggccgcaag atcagcctgc cgggggatct acctacatca ccacttctgt ccgtaggggc
     8701 aagaagctcg ccagagggct cccgttcgcg gaggagtccc tccgggagcg cgaggcaaac
     8761 atgcgggcat cctacgctct cttcgccggg tgtctgggta gcgacagtcc gtatgccgat
     8821 gccatgttca gcgtgttatg agcgccgttc aacaccaggc tctcgtcctc ggcgcgctga
     8881 gtttcgtggc cttcgcgttc ttcgcgtgcg tcgctctctc atccaggaga accaggttca
     8941 gcgtctttgc ggcttgtaac cttctcttcg cgaccctggt gttctgcggc gtagccgcgt
     9001 tcgacctctc ccacgagcgc acggaggtgg tggtggccct catgaccgag ctgcccgtcg
     9061 tgcttatcgt ggcctgggta tgcgtggccc tcgccgggcg gttgccccac ctttctggca
     9121 cgctcccgct ggagcccgcc gcgcgcagcg ccgggttgcg gaagctcctc aggtccgcgc
     9181 cgttcgccct gttgttcttc gtgggtgtcg cctgcgtcgt gggcatggtc ttcccgtcgc
     9241 cagccattcg tgtctacgcg acggcgccca tagagttctt cgtgatgaag agccttatca
     9301 tggtgcctga agcgttctac gcgggcctcg cggcgctcgt cttcggcgtc atggccggac
     9361 gctcgtcgat acccaaacgg cgtctcttct acaagaccct ggccttctcg tcgggcatgg
     9421 cggtcatcgc ccttatcgcg ttggtgtcga tcgtcttcgc gggcgtgcgg gtgtgggtct
     9481 ccgaccagaa ccgcacagcc atcctgaaga tgctcaacac cttcgaagcg tgcctggcca
     9541 tctcgtgcat cgccgtcttc gccctcggcc tctccctgcg gtacacgccc gccgtggccg
     9601 ggcctctgct acagcggctc cagacggggt ggctccatgc tcaggaacaa ttcgagtcgc
     9661 tggaatggcg cgccgtcagc accggcgcgg cgcagcggtt gagggatgcg tccggtaccg
     9721 tagccttggc gtgccgcttt cagaacgtac ccgagccaga gatcgagaaa gccctggcca
     9781 cgataaggct ggtcgccgtc atgaaagatc cctccagtga gacacaacat attacgcccg
     9841 aggcggcacg caaactctac gagcttcagg aggagatcct gggcgacgac gtgctggcct
     9901 ccaggatcag ctgggcggcg ggcctgaggt cccgtgccag ggagccacag accgtaaagt
     9961 ccgcgccgct gcacgacgcg atccgggcgg ccctcgacct gatcgacgac caagacaacg
    10021 aggtccgcat ggagaccaga ccaccctggt actggatcgc cgccgtaagc gcggcggatg
    10081 cgaagatcat tgatcccccc accgccgagt cgttacggtc cgctgaacac gagggatacc
    10141 gtgctgccgc ggacgcgtac cacgcagcaa aatcatccag gcaaaccctg cttggagact
    10201 acccggaacg gaggtccaca tacgataagc cgcgaacgaa ggcaacagag tccaggggag
    10261 gcgaaggcct caccgtcgaa ccagcatctc ggggagagga gtaggtttga agaacgaaga
    10321 cacaaggagc cagcagacgg gtagccacgg tgggccttcc ggccgttctg gcttgttggc
    10381 aatggtcagg aaccgaaccg agagcgagca tgatgcctcg tcgtccgcga ccgacctgac
    10441 gctctccgcg gccggtctcg gacaggcgcc ttacgtggcc gtgggcagcg gcatcgtagt
    10501 agtccagcgc aactgccacc
//
LOCUS       DBNGVD010000045         9375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4561606, whole genome shotgun sequence.
ACCESSION   DBNGVD010000045 DBNGVD010000000
VERSION     DBNGVD010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4561606"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /gene="uvrB"
                     /locus_tag="AC16CM_01190"
     CDS             <1..1470
                     /gene="uvrB"
                     /locus_tag="AC16CM_01190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3138471.1"
                     /translation="DLVRIQYTRNDFQLNRGNFRVKGDVLEVHPAYQDTIYRISFFGD
                     EVENMTEADPLTGEILREHDTLTVYPATHFVTGEDRLAIAVKNIEAEAEERVAELEGE
                     GKVLEAYRLKQRTQYDLEMMRELGYTSGIENYSRHLDGRPAGSTPYTLLDYFPDDYIT
                     FIDESHITLPQVRGMYNGDRSRKTTLVDFGFRLPSALDNRPLMWDEFLLKTSQMVFVS
                     ATPGPYELENSGQIVEQIIRPTGLIDPEVEVRPTKNQIDDLLNEVAKRVEHGERVLIT
                     TLTIKMSEDLTDYLLEHNVKARYMHSNIETLDRIQIIRGLRTGEFDVLVGINLLREGL
                     DLPEVTLVAILDADKEGFLRGERALIQTIGRAARNVKGRVIMYADKETDSMRVAIGET
                     DRRREIQASYNEQHGIEPRTITKGVSDILIAAEAKGLYKAGKKSSREAPRDPDELRTL
                     IADLEAEMLEAAEDLKFEYAAKLRDEIKDLERQLQEVAS"
     gene            complement(1637..2452)
                     /locus_tag="AC16CM_01195"
     CDS             complement(1637..2452)
                     /locus_tag="AC16CM_01195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3138472.1"
                     /translation="MRRFVEERPLVFCVLSTVFLLGTTFLCRAALPAVPSGDLAQLPQ
                     RTFRPPEGIGLVLSDMRSPETLFWALAIGLGLGLLVWLGWWKDAGFTRVSRWRNMRLL
                     GFPLFVCALTLSGGVFSSGIAGLTSTFVVLLVATLGEEIIFRGLLWRALIQKGPLLAV
                     VLTSLLAGVLVLGRTATDGPWPEAVRLAALTFCGGFTYGALRWRTASIWPPIIVHTIF
                     AFVVSISTLGAFRFRLVILLSTVGFIAYGLYLLRNPRVRANGGLAKSHPSRVR"
     gene            complement(2610..3236)
                     /locus_tag="AC16CM_01200"
     CDS             complement(2610..3236)
                     /locus_tag="AC16CM_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR3138473.1"
                     /translation="MVTERRRPLDRRTILEAAVRYVDSEGLEALSMRKLGAELGVEAM
                     SLYNHVPNKEALLDGMVEVLLGELAIPSESEGWENRIKEAYRAFRRLAHEHPNVFPLL
                     VVRPPDTMDGAWLVEEFLKTLGEAGFDPETALYAFRTLSSYASGYAMAEIRGFAMEPA
                     GGRLGASRLPGNEFPHIQELGDRLQRVDHDAEFEFGIDLILTGLRDKL"
     gene            3341..4381
                     /locus_tag="AC16CM_01205"
     CDS             3341..4381
                     /locus_tag="AC16CM_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HIR3138474.1"
                     /translation="MKVSRKDMRWAISEGLISEAQAEDLWLALQGRGVDRPRFDLPHV
                     AYYFGALVVISAMTWFMTLGWERFGGGGMLAISLAYALVFVVAGALLWRESGLRVPGG
                     LLLTAAVCMTPLAVYGFETMTGAWLQGVPGDYADFYSYIKGAWFPMEVATIFAGLVAL
                     RFFRFPFLTAPIAFALWFMSMDLTPLIYGEHYYEAQGYQVVSLVFGLVVLVGAYVVDR
                     RTEEDYAFWGYLFGMFAFWGGLTLLEGGSELAWFFYGLINAGLIVLSVLLQRRVFVVF
                     GAVGVFGYLGHLAWEIFEDSLLFPFALSAAGLTIIALGVLYAKNRDRIEDTIMRTIPA
                     GVRRLLPVERGA"
     gene            complement(4445..4726)
                     /locus_tag="AC16CM_01210"
     CDS             complement(4445..4726)
                     /locus_tag="AC16CM_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138475.1"
                     /translation="MEGPITQATGHNNTIVAVYADRIDIKSGWRSQNVECIGLRDVAL
                     IGIRGLVNVTLTIETNTGRVYRLSRMALPDARRIKNTFEEQKRRAGLYE"
     gene            complement(4847..6256)
                     /locus_tag="AC16CM_01215"
     CDS             complement(4847..6256)
                     /locus_tag="AC16CM_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3138476.1"
                     /translation="MRRLTLISTAVLLFVLALPGLALAQKGPSTAELSLAMDTVWVMV
                     AAVLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLSIAALLFWAFGFALAFGTGNAL
                     VGTEGWFLAVPDGQVNDVYAGLSWTQVPLSAKFLFQVAFCAVSLAIVWGTMLERTKFA
                     VYVVFAVVFAGLIYPMVGHWIWGGGWLTDLGMQDFAGSTVVHLSGAMAALAGTLLLGP
                     RLGKYDDNGRPVTIPGHNMPLAVLGVLILWIGWWGFNPGSTMAAIPDIADIALTTNLA
                     AAAGVLGAMGMSYLYRRHVDVGMGGNGAIAALVAITAPCAFVAPWASIVIGFVAGIIM
                     YATLMYVDRIGVDDPLGAIAAHGMGGIWGTLSCGLFTTPALAAVGEPGLFYGGGLYQL
                     GIQALGIVAAGGFVFVASLAVFAVLKATIGIRVKPEQELDGLDIHEHGVFGYPDLVAT
                     DYQNGNGSRSIPDPVDSTA"
     gene            complement(6544..8277)
                     /locus_tag="AC16CM_01220"
     CDS             complement(6544..8277)
                     /locus_tag="AC16CM_01220"
                     /inference="COORDINATES: protein motif:HMM:NF037653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR3138477.1"
                     /translation="MIRVGYTMLRTERAVILKDFSRILLEELEADCPLPGDATRRVRG
                     LAVDEPLKGWLSEDEVAVTDRETLDEVFLKTVASAGCPAVILRGRDGIDSGAVEWAAD
                     LGLGLFLLAPDVPLRRLFGIFTQDDGLLSLSHGAGRELLEGAGGEARVEDLAGKISSM
                     LGRSVVIEDPVGRLIAGSGGDPDGNRLYATLEEEGLRASRSKSIDETRRERRDRYARL
                     PDGYLSVPVERWRVQEGEMFWTPIGTRAPAGYLWIDFDGANLTPEDVVLLYWARLVLE
                     AELGKERIRLETELGFRGDFVDDLVGGHYGSVELLLQRARYLGADLSRGAMVMVVDID
                     DFAHYLARRRPKEPAIQELKRRLADAVRLQTRQQFSNFLLGPRSDNVILFLGLAEGDE
                     AGELQEKAQLLARGVQRYVKGLLPDLTVSVGIGGYKRDPAQLREAYSEAGVALEIGHR
                     IDGPSSISTFSETGTYKLLFRVLQENPEELEVFYGETLEPVVHYDSRYGTELVQTLIT
                     YLGNDASTVKTAGDLFAHRHTIRYRLDRVGELTGLDVDKSADRERLTLGIKAMQLLGR
                     VPERPSSFSDR"
     gene            8429..>9375
                     /locus_tag="AC16CM_01225"
     CDS             8429..>9375
                     /locus_tag="AC16CM_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3138478.1"
                     /translation="MADNQIVVRGAREHNLKDITVSLPRDVMVVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVAT
                     VTEIYDYLRLLYARAGRPHCHVCGFPVASSTPQQMVEKVLTLPEKTRFLVLAPVVRGR
                     KGEYGKQLNEYAEQGFARVRVDGEVHELPVDLGLDKNYKHDIEVVVDRLVIREGIERR
                     LTDSVETALKLAEGLVQVETVSPDGEGESMLFSESFTCTNCGASIAEIQPRTFSFNSP
                     HGACDRCDGLGSRLEIDPALVVPNEDLSVNEGALVPWANS"
BASE COUNT         1766 a         2953 c         3033 g         1623 t
ORIGIN      
        1 gacctcgtca ggatccagta caccaggaac gacttccagc tcaatcgcgg caacttcagg
       61 gtaaaggggg acgtgctgga ggtgcacccc gcctaccagg atacgatcta ccgcatctcc
      121 ttcttcgggg acgaggtcga gaacatgacg gaggccgacc ccctgacggg tgagatactg
      181 cgcgagcatg acacccttac cgtctacccc gcgacccact tcgtgaccgg tgaggacagg
      241 ctcgcgatcg ccgtcaagaa catcgaggcg gaggcggagg aacgcgttgc agagctcgag
      301 ggcgagggca aggtgcttga ggcgtaccgt ctcaagcagc gcacccagta cgacctggag
      361 atgatgcgcg agctgggcta cacctcgggt atcgagaact actcccgtca cctcgacggc
      421 cggcccgccg gctcgacgcc gtacaccctg ctcgactact tccccgatga ctacattacc
      481 ttcatcgatg agtcgcatat aacgctgccc caggtcaggg gcatgtacaa cggggacagg
      541 agcaggaaga ccaccctggt cgatttcggc ttcaggcttc cttctgcgct ggacaacagg
      601 ccgctgatgt gggacgagtt cttgctcaag accagccaga tggtcttcgt ctctgcgaca
      661 ccggggccgt acgagctgga gaactcgggc cagatagtgg agcagatcat ccgcccgacc
      721 ggcctcatcg acccggaggt cgaggttcgc ccgacgaaga accagatcga cgaccttctg
      781 aacgaggtcg ccaagcgcgt ggagcacggc gagcgggtcc ttatcaccac gctcaccata
      841 aagatgtcag aagatttgac ggactatcta ctggagcaca acgtcaaggc ccgctacatg
      901 cactccaaca tagagaccct ggaccggatc cagatcatcc gcggtctacg caccggcgag
      961 ttcgacgtgc tggttggcat caacttgctc cgcgagggac tcgaccttcc tgaggtcacg
     1021 ctagtcgcca ttctggatgc ggacaaggaa ggcttcctgc gcggggagcg ggccctcatc
     1081 cagaccatcg gccgggccgc gcgaaacgtg aaagggcggg tcatcatgta cgccgacaag
     1141 gagaccgact ccatgcgcgt cgccattggg gagacggatc gccggcgcga gatccaggcc
     1201 tcctacaacg agcagcacgg catcgagcca cgcaccataa ccaagggcgt ctcggatatc
     1261 ctcatagccg ccgaggccaa aggactctac aaggcgggca agaagtcgag ccgcgaggcc
     1321 ccgcgcgatc cggacgagct ccgcacgctt atcgccgacc tggaagccga gatgctagag
     1381 gccgccgagg acctcaagtt cgagtacgct gcaaaactca gggacgagat caaagacctc
     1441 gagcggcagc tgcaggaggt tgcttcctag ggctgaactg cgctacccga tagaacctcg
     1501 ggtcttggga cgggcttctt cgcggtaacg gacgagacaa gcgcacggtc cccatgctgt
     1561 ttggtagctt tcgtgcctgc ctggaggata cgttcggctt cgagcctccg gtaagtatcc
     1621 tcgatggtcg cgattcttac cttacccgtg acggatgcga cttcgcgagg ccaccgttcg
     1681 cgcggacgcg ggggttgcga agcagataga ggccgtaggc gatgaacccg acggtgctga
     1741 gcagtatgac gaggcggaac ctgaaggcac caagagtgga gatgcttacc acgaaggcga
     1801 agatggtgtg tacgataatg ggcggccaga tggaggcggt gcgccagcga agcgcgccgt
     1861 aggtgaagcc gccgcagaag gtcagggccg cgaggcggac ggcttctggc cacgggccat
     1921 ccgtcgccgt tctgccgagc accagcacac ccgcgagcag ggaggtcagg accacggcca
     1981 gcagtggccc tttctgtatg agcgcccgcc acaggagccc gcggaagatg atctcctcgc
     2041 cgagggtcgc aacgagcaag acgacgaacg tggaggtgag gcccgcgata cccgagctga
     2101 agacgccccc tgaaagggtc aacgcgcaca cgaacagcgg aaagccgaga agccgcatgt
     2161 tccgccaccg cgagaccctc gtgaagccag cgtccttcca ccagccgagc cagacgagca
     2221 agcccagccc gagccctata gccagagccc agaacaacgt ctccgggctc ctcatgtccg
     2281 agaggaccag gccgatcccc tccggcggcc ggaaggttct ctgcggcaac tgtgcgaggt
     2341 ctcccgacgg aacggccggc aaggccgccc tgcacaggaa cgtggtcccg agcaggaaca
     2401 ccgtgctcag aacacagaac accagaggac gctcttcgac gaaacgcctc accgccgaat
     2461 gcctccagac gaactgttgc acgaatctcg gaccccacgc gtacgaaata cgtagccgat
     2521 gacgcacctc ataacgggcg agatccacaa gaggtgaggc cccagaaacg aatccgtgat
     2581 gcctgatacc cgatgcctgc cacctccgct cagagcttat ccctcagacc ggtgaggatg
     2641 aggtcgatgc cgaactcgaa ctcggcgtcg tggtctaccc tctgcaggcg gtcgcccagc
     2701 tcctggatgt gtggaaattc gttgccgggc agcctggaag caccgagtcg tccgcccgcg
     2761 ggctccatgg caaaaccccg gatctccgcc atcgcgtacc cggacgcgta gctggagagc
     2821 gtccggaaag cgtagagtgc cgtctcgggg tcgaagcccg cctcgccgag cgtcttcaaa
     2881 aactcctcca cgagccaggc cccgtccatg gtgtccggcg gccggacgac gagcagcggg
     2941 aacacgttcg gatgctcgtg ggcgagccgc cggaacgccc ggtacgcctc cttgatgcgg
     3001 ttctcccagc cctcactctc cgacggtatt gcaagctcgc ccagcaatac ctctaccatg
     3061 ccgtcgagca aggcctcctt gttcggcacg tggttgtaga gcgacatcgc ctcgaccccc
     3121 agctcggccc caagcttccg catcgaaagc gcctccaggc cctcgctgtc cacgtaccgt
     3181 accgccgcct ccagaatggt ccgcctgtcc agcggccgcc ttctctccgt taccacaagg
     3241 gaacctccat ccaccagaaa ccttgacagc ttacagcata agccttaagc ttacgacgta
     3301 tacttacggt gtaagtaaag cagagaaaag gggtgatgag gtgaaggtga gcaggaaaga
     3361 catgaggtgg gcgatctcgg aggggctcat ttccgaggcc caggcggagg acctctggct
     3421 agcgctccag gggcgtgggg tggatcgtcc gcggttcgac cttccgcacg tcgcctacta
     3481 cttcggggcg ctggtcgtta tctcggccat gacctggttc atgacccttg gttgggagag
     3541 gtttggcggt ggggggatgc ttgcgatctc cctcgcctac gcgctcgttt ttgtggtcgc
     3601 tggcgctttg ctgtggcgcg agagtggtct gagggtccct ggcgggctcc tccttacggc
     3661 ggccgtgtgc atgacgcccc tcgccgtcta cggcttcgag accatgacgg gggcgtggtt
     3721 gcagggggtg cccggggatt acgcggattt ctactcgtac atcaagggtg catggttccc
     3781 catggaggta gcaacgatct tcgccggcct cgtcgcgctc cggttcttcc ggttcccgtt
     3841 cttgaccgcg cctatcgcct tcgcgctgtg gttcatgtcc atggacttga cgccgctcat
     3901 ctacggcgag cactactacg aggcgcaggg ctatcaggtc gtatccctcg tcttcggtct
     3961 ggtcgtgctg gtcggcgcgt acgtggtgga ccggcgtacg gaggaggact acgcgttctg
     4021 gggttacctg ttcgggatgt tcgccttctg gggaggtctc acccttctgg aggggggtag
     4081 cgagcttgcc tggttcttct atggtctcat caacgcgggc ctcatcgtgc tctccgtgct
     4141 attgcagcgg cgggtcttcg tcgtattcgg cgcggtgggg gtgttcggtt acctgggaca
     4201 tctcgcctgg gagatcttcg aggattcgct cctctttccg ttcgccctct cggcggccgg
     4261 gctcaccatc atcgccctcg gtgttctcta cgcgaagaac cgtgacagga tcgaggacac
     4321 cataatgcgc acgatcccag ccggcgtcag acgccttctc cccgtagaac gaggggcgta
     4381 acaccctcgt aggggcggtt cgcgaaccgc ccctacaggt ttggggggga cacgggccgg
     4441 gatcttattc gtagaggccg gctcttctct tctgctcttc gaaggtgttc ttgatcctgc
     4501 gggcatccgg gagggccata cggctcagcc tgtagacgcg cccggtgttg gtctctatcg
     4561 tgagcgtgac gttgaccagg cccctgatgc cgatcagggc cacgtcgcgc aggcctatgc
     4621 actccacgtt ctgggaccgc cagccggact ttatgtcgat tcggtcggcg tagacggcca
     4681 cgatcgtgtt gttgtggccg gtggcctgcg ttataggtcc ttccaagctg ccctccgtac
     4741 cgctcgcggt ccgtgaccag catacaagac tgcgccacga atgttgtcgg gtcgacgtga
     4801 aaaagcaccg gtcggaggag atccgaccgg tgcagatatt ggttacctag gccgtgctat
     4861 ctacggggtc ggggatgctc ctgctcccgt ttccgttctg gtagtcggtg gcgaccagat
     4921 cggggtaccc gaagacgccg tgttcgtgga tatcgaggcc gtcgagctcc tgctcgggct
     4981 tgaccctgat gccgatggtc gccttcagga cagcgaagac cgccagggag gcgacgaaca
     5041 cgaagccgcc ggcggccacg ataccgaggg cctgaatacc gagctggtag aggccgccac
     5101 cgtagaacag gccgggctcg ccgaccgcgg ccagggccgg ggtggtgaac aggccgcagg
     5161 agagcgttcc ccagataccg cccatgccgt gcgcggcgat ggcgccgagg gggtcgtcca
     5221 ccccgatcct gtccacgtac atgagcgtcg cgtacatgat gatgccggcg acgaagccaa
     5281 taacgatgga ggcccagggc gcgacgaagg cgcacggcgc cgtgatggcg acgagggctg
     5341 cgatggcgcc gttgccgccc atccccacgt cgacgtgtcg cctgtagagg tagctcatcc
     5401 ccatcgctcc cagaacgcca gcggccgcgg ccaggttggt cgtgagggcg atgtctgcga
     5461 tgtcggggat ggcggccatc gtcgagccag ggttgaagcc ccaccagccg atccagagta
     5521 tcaggacgcc gagaacggcg agcggcatat tgtgcccggg gatggttacc ggacgtccgt
     5581 tgtcatcgta cttgccgagg cggggtccca ggagcagggt accggcgagc gcggccatgg
     5641 ctccggagag gtggaccacc gtcgagccgg cgaagtcctg catgcccagg tccgtcaacc
     5701 agccgccgcc ccagatccag tgcccaacca tggggtagat caggcctgcg aagacgaccg
     5761 cgaagaccac gtatacggcg aacttggtcc gctccagcat ggtgccccac acgatggcga
     5821 gggagaccgc gcagaaggcg acctggaaga ggaacttggc cgagagcggg acctgcgtcc
     5881 aggagagccc cgcgtacacg tcgttgacct gcccgtcggg tacggcgagg aaccagccct
     5941 ccgtaccgac gagcgcgttg cccgtgccga aggccagcgc gaacccgaag gcccagaaca
     6001 gcagggcggc gatcgacagg ttgaccagga tcttggcgac cacgctgccg acgttcttca
     6061 tccgcgagaa cccgacctcc agcatcgcga acccggcctg catgaacaga accaggaccg
     6121 cggccaccat cacccacaca gtgtccatcg ccagcgagag ctccgccgtg gaaggacctt
     6181 tctgggcaag agcgagcccc ggcaacgcga gcacgaataa aagtaccgcc gtagatatca
     6241 acgtgagccg ccgcaaaagc ttcctcctct tcagggtata tacgtatgga acatagtcta
     6301 ggcagcgagt tcggaagggt ctttagccga acgtataaag atggagcatc ctcaaggggg
     6361 tgccgttgcg ctggatgtac aaggaaggct acaggtttca ggctacaggt cttgtcgaga
     6421 atgcaccttt gtggcggtcg cccgacgttc tgaccgtgga gacgccacgg gtaggggtgc
     6481 ggctgacccc agggtcgtgt cggcggcagc cttacccgaa gcctgaaacc tgtagcctga
     6541 tgcctatcgg tccgagaagc tggatgggcg ctccgggaca cgaccgagaa gttgcatggc
     6601 ttttatgcct agcgttagac gttcgcggtc tgcgctcttg tccacgtcga ggcccgtcag
     6661 ttcgcccacg cggtccaggc ggtagcggat ggtgtgtcgg tgggcgaaga ggtctccggc
     6721 agtctttacg gtggaggcgt cgttgccgag gtaggttatg agggtctgga ccagctccgt
     6781 gccgtagcgg gagtcgtagt ggactacggg ttccagcgtc tccccgtaga agacctcgag
     6841 ctcttcgggg ttctcctgca gaaccctgaa gagaagcttg tatgttccgg tctcgctgaa
     6901 ggtcgagatg gaggacgggc cgtctatacg gtgaccgatc tccagcgcca cgcccgcctc
     6961 ggagtatgcc tcgcggagtt gggcgggatc cctcttgtac ccgccgatgc cgacggagac
     7021 ggtcaggtcg gggagaagcc ctttcacgta gcgctgcaca cccctggcca gcagttgcgc
     7081 tttctcctgt agctcgccgg cttcatctcc ctcggcgagg ccgagaaaca ggatgacgtt
     7141 gtcggatcgt gggccgagca gaaagttcga gaactgctgg cgggtctgca ggcgcacggc
     7201 gtcggccagg cggcgcttga gctcctgtat ggccggctcc ttcggtcggc ggcgggcaag
     7261 gtagtgggcg aagtcgtcta tgtcgacgac catcaccatg gcgcccctgg agaggtcggc
     7321 gccgaggtaa cgggcgcgct gcaagaggag ctcgacggaa ccgtaatggc ctccgaccag
     7381 gtcgtctacg aagtcgccgc ggaagcctag ctccgtttca agacgaatgc gttccttgcc
     7441 caactctgcc tccagaacga gcctcgccca gtacaggagg accacgtcct ccggcgtcag
     7501 gttggcgccg tcgaagtcga tccacaggta tcccgccggc gccctggtgc cgatgggggt
     7561 ccagaacatc tcaccctcct gcacgcgcca gcgctccacg ggtacggaga ggtagccgtc
     7621 ggggagccgg gcgtagcggt cgcgcctctc ccggcgggtc tcgtcgatgc tcttgctcct
     7681 cgaggcccgc aggccttcct cttcgagcgt cgcgtagagg cggttgccat ccgggtcacc
     7741 tccggagccg gcgatcagac gtcctactgg gtcctctatg acgacggagc ggcctagcat
     7801 gctggagatc ttgcctgcca gatcctcgac cctggcctcg ccccccgcgc cctccaggag
     7861 ctccctgccg gcgccgtggg agagcgagag caatccgtcg tcctgggtga agatcccgaa
     7921 gaggcgcctg agtgggacgt cgggggcgag caggaacagg ccgagcccca ggtcagcggc
     7981 ccactccacg gccccggaat cgatcccgtc ccttccccgc aggatgacgg cggggcagcc
     8041 ggcggaggcc acggtcttca ggaagacttc gtcgagcgtt tcgcggtccg tcacggccac
     8101 ttcgtcctcg gagagccagc ccttcagggg ctcgtcgacg gcgagcccac gcacccgcct
     8161 ggtagcgtcc ccgggcagcg ggcaatcggc ctccaactcc tctagaagga tgcgggagaa
     8221 gtccttgagg atcacggcac gctccgttcg caacatggtg taaccgacgc gtatcatttt
     8281 ggccccccgg accaaaaatc gcaaacgcac acacagaggg gcaccccgcg ggtggtagaa
     8341 tcttcgtcct acggaaactg cttctttggg ctcccgacag ggggccgtat acttgctgga
     8401 agcgtctcgt aaagagggga gaagagagat cgcggacaac cagatcgtcg tgcgcggggc
     8461 gcgcgaacac aacctcaagg acataacggt ctccctgccg cgcgacgtga tggtggtcgt
     8521 gaccgggctt tcgggaagcg gcaagtcctc cctggccttc gacaccatct acgcggaggg
     8581 ccagcgtcgc tatgtggaga gcctctcggc gtacgcgagg cagttcctcg gccagatgga
     8641 caagccagac gtggaccaca tcgacggcct ctcaccggcc gtctccatcg accagaagac
     8701 cacctcgaac aacccgcgct ccaccgttgc gacggtaact gagatctacg actacctccg
     8761 cctgctgtat gcccgagctg gcaggccgca ctgccacgtc tgtggtttcc ccgtggcttc
     8821 ctccacgccg cagcagatgg tcgagaaggt gctgacgctt ccggagaaga cacgctttct
     8881 cgtgctcgcg cccgtggtgc gggggcgcaa gggcgagtac ggcaagcagc tcaacgagta
     8941 cgccgagcag ggtttcgccc gcgtccgggt ggacggggag gttcacgagc tacccgtcga
     9001 cctcggtctg gacaagaact acaagcacga catcgaggtc gtggtcgaca ggctcgtgat
     9061 ccgggagggt atagagcgcc ggctaacgga ctcggtggag acggccctca agctagccga
     9121 ggggctcgtc caggtcgaga cggtgagccc cgatggggag ggcgagtcga tgctcttctc
     9181 cgagagcttt acgtgcacga actgcggcgc ttcgatcgct gagatacagc cgcgcacgtt
     9241 ctcgttcaac agcccgcacg gggcctgcga ccgctgcgat ggcctcggaa gcaggctgga
     9301 gatagacccg gcgctcgtcg ttccgaacga ggacctgagc gtaaacgagg gggccctggt
     9361 gccgtgggcc aactc
//
LOCUS       DBNGVD010000046         4930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2922680, whole genome shotgun sequence.
ACCESSION   DBNGVD010000046 DBNGVD010000000
VERSION     DBNGVD010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2922680"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..697)
                     /locus_tag="AC16CM_01230"
     CDS             complement(14..697)
                     /locus_tag="AC16CM_01230"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HIR3138479.1"
                     /translation="MTEHQELRNLLGPYVMGSLEAREDRKVEGHLRECARCREEASGL
                     RLAHERLLDLAYTTEFPPLKPEDRAVGAAISHRQRLRRTAVRAAVTAAFVFVLVVFGA
                     ILVPDLSGGGALASATLTPTDLAPDAGGHISIGDANGNTEVSLKAWGLPSCEGGDYFE
                     LWLVEGEERVSAGSFTVGPGGEIDINMNAPGFVGTYPSVGITREHDEDPRASDADVLS
                     GKLRRGPQE"
     gene            complement(690..1364)
                     /locus_tag="AC16CM_01235"
     CDS             complement(690..1364)
                     /locus_tag="AC16CM_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3138480.1"
                     /translation="MKGLSSGKAARALPGGGRGIICPEGRRAGASVGGPGRACMDRVA
                     DEVLVRRVASGDEEALSNLYDRYAGLVYGTGKRLLGDRETAEELVQDVFTSVWRNSAD
                     FDPARAHFATWLYRITRNRATDQVRRRNARVRVVGREPLLERGEADPSGTLSRRFDVA
                     AALSRLSPVHREILVLAYFRGLTQREISERTNMPLGTVKSRTTAALRALRKTMSIDGD
                     RRPVDD"
     gene            1464..2498
                     /locus_tag="AC16CM_01240"
     CDS             1464..2498
                     /locus_tag="AC16CM_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3138481.1"
                     /translation="MVPVASDTTGTKLGGVARRPARGSPGQFFRDLRDLLRYDTLKAT
                     VQEFIRDDALGLAAQLAFYLILAIFPFILFCVAILDAFSSSDPQFVTDLFFYLKRLLP
                     AQVYALIHTYTENTLRNSDTAPGLLSVGILGTVWAASGAFAALINALNRAYDVQETRP
                     YWKVRGIAVLMTFGLSALLLAGVLLLVAGPSIGSTIARVFTLDDEFMIAWNIARWPAA
                     LLFMVSTVALLFYFAPDANQPFRWITPGGVIGIVLWILTSVAFNLYLSSDFNTYDRTY
                     GSIGTVIVLLLYLYISSLTILFGATLNATLVRIKEEVSGERILEAEPANLKPDVVDEE
                     TVEQIRDDEG"
     gene            2549..2638
                     /locus_tag="AC16CM_01245"
     tRNA            2549..2638
                     /locus_tag="AC16CM_01245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2583..2585,aa:Ser,seq:gct)
     gene            2850..3869
                     /locus_tag="AC16CM_01250"
     CDS             2850..3869
                     /locus_tag="AC16CM_01250"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR3138482.1"
                     /translation="METRTIKPLTRRLCEGALGLYGLRDAHLEPLVGGFVEVYLVRTA
                     DGEDFVLRLYGLSHVDEAAMREDPRLRTGPGLRSPDTIHAQLLWVASLGRETGLLVPE
                     PMLTTEGSLVGRVSVEGMQTERHCALLRWVPGLHRAKDPSPEDLSLLGSFVGRMHDHS
                     ERHPLPEPVALPRWDHSWPFGDSAPLWDEGQRFYSEEDMGVFVETARHSKEILEELGE
                     GADVFGVIHRDLKLDNVVFHRGKAGVLDFDACGLGHYLLDLCRVRRNLTSTHPNRLRE
                     LWNAFMGGYEATRPLPEGHRGYFGTFAAMQTVAAVNRQVALLATGAPGRRRETRASSR
                     TPLGC"
     gene            complement(4129..4329)
                     /locus_tag="AC16CM_01255"
     CDS             complement(4129..4329)
                     /locus_tag="AC16CM_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138483.1"
                     /translation="MTLTITHEKTREVTREDVDRAIDVIERHLEKEARTRNLLDEQAI
                     MRPKDLCKQQGLDMVAELRGMM"
     gene            complement(4481..>4930)
                     /locus_tag="AC16CM_01260"
     CDS             complement(4481..>4930)
                     /locus_tag="AC16CM_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR3138484.1"
                     /translation="VLASEVPPGSTVALSTTNGTRIVEAASGAQAIFAGAFVNAESLA
                     NHLCEALRDHPEVAVIGCGWEGHRASEDEVAAGAILHRLETRGAELDGRARRVVERYL
                     SRPTSTLAANSAARRLECLGYGRDLAFCLAEDTVPVVPRLVGGAFVG"
BASE COUNT          859 a         1662 c         1537 g          872 t
ORIGIN      
        1 ctggggagat gccctactcc tgcgggcccc ggcgcagctt gccggagagg acgtccgcgt
       61 cgctggcgcg ggggtcttcg tcgtgctcac gggttatccc gacggagggg taggtaccga
      121 cgaagccggg ggcgttcatg tttatgtcga tctccccgcc cgggccgacg gtgaagctgc
      181 cggcgctgac gcgctcctcg ccctcgacga gccacagttc gaagtagtcg ccgccctcgc
      241 aagaaggcag gccccaggcc ttcaggctga cctccgtgtt cccgttggca tctcctatgc
      301 tgatatgacc gcctgcatcg ggggcgagat ccgtcggggt cagagtcgcc gaagcgaggg
      361 ccccaccacc ggagaggtcg ggcacgagga tggcaccgaa cacgaccagc acaaaaacga
      421 aagcggccgt cacggccgcc cggaccgctg tacgtcgcag cctctggcgg tgggagatcg
      481 cggcgcccac ggcgcgatcc tccggcttca gcggggggaa ttccgtggta tacgccaggt
      541 cgaggaggcg ttcatgggcc agcctcaacc cgctcgcctc ctccctgcaa cgcgcgcatt
      601 ctctgaggtg accctcgacc ttcctgtcct ctcgcgcctc aagggaaccc atcacgtagg
      661 gaccgaggag gttccgcagc tcctgatgct cagtcatcca cgggccgccg atccccgtcg
      721 atactcatgg ttttgcgcaa ggcgcgcagg gccgccgtgg tacggctctt gaccgtacca
      781 agcggcatgt tcgtacgctc ggagatctca cgctgtgtga ggccccggaa gtaggcgagc
      841 acgagaatct ccctgtgaac cggggacagg cgcgagagcg cggcggccac gtcgaagcgg
      901 cgcgagagcg tgccggacgg gtcggcctcc ccccgttcga gcagcggttc cctccctacc
      961 accctcacgc gcgcattgcg gcgacggacc tgatccgtcg ccctgttgcg ggttatgcgg
     1021 tagagccagg tcgcgaagtg cgcccgcgcc gggtcgaaat cggccgagtt ccgccacacc
     1081 gaggtaaaga cgtcctggac cagctcctcg gcggtctccc tgtcgcccag cagacgcttg
     1141 cccgtcccgt agaccaggcc agcgtagcgg tcgtagagat tcgagagggc ctcctcgtcc
     1201 cccgaggcca ccctcctgac cagaacctcg tctgccacac ggtccataca ggcccttccg
     1261 ggaccgccaa cggaagcccc ggcacggcga ccctcggggc agatgatacc ccgcccgccg
     1321 ccaggcaaag cccgcgccgc cttccctgat gacaacccct tcatacgggg atttatacga
     1381 ccggccgcgc cccgcggatt agcgtttccg cgcacgaggt ccacgatcac gggtaaaata
     1441 agcgccattg cgagaaggga gacatggtcc cggtggcgtc cgacacgacc ggcacgaagc
     1501 tcgggggggt ggcccgcagg ccagcgcgcg ggtcaccggg ccaatttttc agggatctca
     1561 gagatctcct gcgctacgac acgctcaagg ccaccgtgca ggagttcatc cgcgacgacg
     1621 cccttggcct agccgcccag ctggccttct acctgatact ggcgatcttc ccgttcatcc
     1681 tgttctgcgt ggccatactc gacgccttct ccagctccga cccgcagttc gtcacggacc
     1741 tcttcttcta cctgaagcgg ctcctgccgg cgcaggtcta cgccctgatc cacacctaca
     1801 ccgagaacac gctgcgcaac agtgacaccg ccccaggcct cctctccgtc ggcatccttg
     1861 gcacggtctg ggcagcctcc ggtgccttcg ccgccctgat aaacgccctg aaccgcgcct
     1921 acgacgtgca ggagaccagg ccgtactgga aggtgagggg catcgccgtg cttatgactt
     1981 tcggcctctc ggcgctgctg ctcgcgggcg tgctcctcct ggtcgcagga ccctccatcg
     2041 ggagcactat cgcccgggtc ttcaccctgg atgacgagtt catgatcgcg tggaacatag
     2101 cccgctggcc cgcggcgttg ctcttcatgg tcagcacggt ggcgcttctc ttctactttg
     2161 ccccagacgc caaccagcct ttccgctgga tcacaccggg cggcgtgatc ggcatcgtgc
     2221 tctggatact gacgagcgtg gccttcaacc tctacctgag cagcgacttc aacacctatg
     2281 acagaaccta cggctccata ggcaccgtga tagtcctcct cctctacctc tacatctcct
     2341 ccctgaccat cctcttcgga gccaccctga acgccaccct ggtgaggatc aaagaagagg
     2401 tctccgggga gcggatcctg gaggcagaac cagcaaacct caaaccagac gtggtagacg
     2461 aagagacggt cgagcagatc agagacgacg aaggttagca aggacccggg cagacacccc
     2521 acacgtcttt gatatactgt gcttatctgg aggagtggcc gagtggttga aggcggcacc
     2581 ctgctaaggt gttatacctc tcaacggggt atcgagggtt cgaatccctc ctcctccgcc
     2641 ccgtaagctg gggaacgaaa cgcccctggg cccgcccccg ccccctcgtt ctactccggg
     2701 gacccttaca tctagtcctc cccaccccca cagttgccgg agcccacatt agaggatcct
     2761 aatcgtctgt cggacccggc ctcgacgttc gcatgctagg gtatccccca ggatagaagg
     2821 ccgggatgtc gggaggtaga ggaactctgg tggagacaag gaccatcaaa ccgctgacaa
     2881 ggcgtctgtg cgagggcgca ctcgggcttt acggtctgcg ggatgcccac ctcgagccgc
     2941 tcgtaggcgg cttcgtggag gtttacctcg tgcggaccgc agacggggaa gacttcgtcc
     3001 tccggctcta cggcctgtcc cacgtcgacg aagcggcgat gcgcgaggac cccaggcttc
     3061 gtaccggacc gggcctacgc tccccagata cgatccacgc gcagttgttg tgggtggcct
     3121 cgctcggacg cgaaacaggg ctgctcgtac ccgaaccgat gctcacgacg gaaggctcgc
     3181 tagtgggacg ggtatcggtc gaggggatgc agaccgaacg ccactgcgcg ctcttgcggt
     3241 gggtgcctgg cctccacagg gccaaggacc cttcccccga ggacctctcg ttgttgggtt
     3301 cgttcgtagg cagaatgcac gaccactcag agcgccatcc cctacccgaa ccggtggccc
     3361 ttcccaggtg ggatcattcc tggccgtttg gggattcggc ccccctgtgg gatgagggtc
     3421 agcgtttcta ctcggaggaa gacatggggg tgttcgtgga aacggcacgg cactcgaagg
     3481 agatcctgga ggaactgggc gagggcgcgg acgtgttcgg cgtcatccac cgcgacctga
     3541 agctagacaa cgtcgtgttc caccgcggga aggcgggcgt cctggacttc gacgcctgcg
     3601 gtctgggcca ctatctcctg gacctctgcc gggtgcgcag gaacctcacg agcacacacc
     3661 cgaaccgcct cagggaactc tggaacgcct tcatgggagg ctacgaggcc acgcgccccc
     3721 ttcccgaggg ccatcgcggg tacttcggaa cgttcgccgc gatgcagacg gtggcggccg
     3781 tcaacaggca agtcgcgctg ttggcaaccg gggccccagg aagaagacgc gagacccgcg
     3841 cttcctctcg aacgccgttg ggttgctgaa gaggatgtcc gcgaactggg cgctcttgcc
     3901 tcttggtatg caggagctac cgtacaaact gggcgccgtt atcggggcgg catgatccgc
     3961 tgtcccggcg cgaggcagaa ccgcaggagc cctggcgtgc aaaaagggcc ggatctctcc
     4021 ggcccttgcc tggccacgtg aagggggatg gacagccctc gggcgcccgt ttgggacgca
     4081 ggggttttta ctgtgcatcc cccttcatgg gccttaggta acgcggaatt acatcatgcc
     4141 gcgcaactcg gcaaccatat ccagtccctg ctgcttgcac aggtccttgg ggcgcatgat
     4201 cgcctgctcg tcgagcaggt tgcgggtcct ggcctccttc tcgaggtggc gctcgatgac
     4261 gtcgatagcc cggtccacat cctcgcgggt gacctcgcgg gtcttctcgt gggtgatagt
     4321 gagagtcact tgtctagcac ctccttcccg ttgcggatta gacggagttt gtaacatttg
     4381 atccgccttc gctaactcca gagtaatcgc ggaccaccgc gctgtcaaga acaattttcg
     4441 gcttcggacg ttggccctcg ggccgaaaat ccgtggttgt ttaccccacg aaagcgccgc
     4501 cgacgaggcg gggtacgaca ggcaccgtgt cctcggccag gcagaacgcc agatcccttc
     4561 catagccgag acattcgagt ctccgcgccg cgctgttggc ggcaagcgtg ctcgttggcc
     4621 tggacagata acgctcgacg acacgcctcg cccgcccgtc gagctccgca ccgcgcgttt
     4681 cgagccggtg gaggatggcc ccggccgcaa cctcgtcctc cgaggcacgg tgaccctccc
     4741 agccgcaccc gataaccgca acctccgggt ggtcccggag agcctcgcac aggtgattcg
     4801 cgaggctctc cgcgttgacg aaggcgccgg cgaagatggc ctgcgcgccg gaggcggcct
     4861 cgacaatgcg ggtgccgttc gtggtggaga gggcgaccgt ggatcccgga ggtacttcgg
     4921 aggcaagtac
//
LOCUS       DBNGVD010000047         1874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2193730, whole genome shotgun sequence.
ACCESSION   DBNGVD010000047 DBNGVD010000000
VERSION     DBNGVD010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2193730"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..343
                     /locus_tag="AC16CM_01265"
     CDS             110..343
                     /locus_tag="AC16CM_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3138485.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIFPEDEVPDEMESYTTKAANYFE"
     gene            522..1094
                     /locus_tag="AC16CM_01270"
     CDS             522..1094
                     /locus_tag="AC16CM_01270"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3138486.1"
                     /translation="MDDGSWRTIGLDYLYQSPWCAFRLDDVELPGGERIEYGVVEGGE
                     FAAVVPVTCGGDVVLVRQWRQPLGAFTLELPSGGVDAGEEPDLAASRELFEETGFRAE
                     NLQHLVSMHTSPGRSTEVCHLYRCDAVPDEQGPRPEPTEFIQVVQMPLQEARDMVSGA
                     EITAASTVLGLLLVGGGLNWGRKQTGEPQG"
     regulatory      1066..1166
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1202..1756
                     /gene="thiT"
                     /locus_tag="AC16CM_01275"
     CDS             1202..1756
                     /gene="thiT"
                     /locus_tag="AC16CM_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR3138487.1"
                     /translation="MNAFRDTRVLTEAAIAVALSFVLGLFVLFKMPFGGSISLEMIPL
                     ILLSLRQGWRVGVVAGVTYGLLDLAIDPFVVHPLQLLLDYPLAFGVLGLAGLFKPTVR
                     GAVLGATVAVLARFVCHFLSGVVFFASYAPEGWNPYIYSAAYNAAYLVPSLLIAIAVV
                     VVLMKALDAAQPSPRQVRYRSSTT"
BASE COUNT          325 a          616 c          601 g          332 t
ORIGIN      
        1 acgcatgatc ccgatcatgg gcggcgatga tgctcgtcaa tggagggttt tgggaccgga
       61 aggtcgctgg tataaagccg ctaccagaga gtttcgagag gaggaacctg tgacctatgt
      121 gatcacggag ccgtgtatcg gcacgaagga ccagagctgc gtcgaggtct gcccggtgga
      181 ctgcatctac gacgccggcg accacttcat gatcaacccc gaggagtgca tcgactgcgg
      241 cgcctgcgag ccagagtgcc ccgtcgaggc catcttcccc gaggacgaag tccccgacga
      301 gatggagagc tacaccacca aggccgccaa ctacttcgaa tgacaaaccc ccgatacaca
      361 aggtaggggc ggttcgcaaa ccgcgcccgc ccagaggagg gacccgtagg tccctcctct
      421 tgttgtgcga ccccaatcac acccaccccg agcaacacaa gaacccataa catgcagggg
      481 cggttcgaac cgcccaacgc cgaaccccta tactcccacc catggatgac ggcagctggc
      541 gaactatcgg gctggactac ctctaccaga gtccctggtg cgccttccgc ctcgacgacg
      601 tggagttgcc tggcggggag aggattgagt atggcgtcgt ggagggcggc gagttcgccg
      661 ccgtcgtccc tgtcacgtgt gggggagacg tggttctcgt gcggcagtgg cgccagcccc
      721 tgggcgcctt cacgctggag ttgccgagcg gcggggtgga cgccggcgag gagccggatc
      781 ttgcagccag cagggagctt ttcgaggaga ccggcttcag ggcagagaac ctgcagcacc
      841 tggtctccat gcataccagc ccgggccgga gcacggaggt ctgccacctg tatcggtgtg
      901 atgccgtacc ggacgagcaa ggtccgcggc ccgagcccac ggagttcata caggtcgtgc
      961 agatgcccct gcaagaagcc agggacatgg tctccggggc agagataacc gccgcctcca
     1021 ccgtgctcgg gttgctactc gttggcggtg ggttaaactg ggggcgtaaa caaaccgggg
     1081 agccgcaagg ctgagagggc acgtggtgcc gacccgctga acctgatccg ggtaatgccg
     1141 gcgcagggag attctagagt cttgtcggcg tatccccgca cacaccgagg aggatacgcc
     1201 cgtgaacgct ttcagggaca ccagggtgct taccgaggcg gccatcgccg tcgcgctgtc
     1261 ctttgtgctt ggacttttcg tgctcttcaa gatgcccttc ggcggctcca tctcgcttga
     1321 gatgatcccc ctgatactcc tctccctcag gcagggctgg agggtcgggg tcgtcgccgg
     1381 cgtgacctac ggcctcctcg acctcgccat cgaccccttc gtggttcacc cgctgcaact
     1441 cctgctcgac tacccgctcg ccttcggtgt cctcgggctc gccgggctct tcaagccaac
     1501 cgttcggggc gcggtcctag gcgccacggt cgccgtgctg gcgcgctttg tctgccactt
     1561 cctctccggg gtggtcttct tcgcctccta cgcgccggaa ggttggaacc cttacatcta
     1621 ctcggccgcc tacaacgcgg cgtatctggt cccgagcctc ctcatcgcca tagcggtcgt
     1681 ggtcgttctt atgaaggcgc tcgatgcggc ccagccctcg ccgaggcagg tacgctaccg
     1741 tagctcgacg acctagggag gcaggcatgc gggccgcgat cttcctgaac ggagcccccg
     1801 acccggagcc cctgatccgg gccctggccg gcagggccga cctcgtggtg gccgcagacg
     1861 gcggggcccg ctac
//
LOCUS       DBNGVD010000048         3849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4015638, whole genome shotgun sequence.
ACCESSION   DBNGVD010000048 DBNGVD010000000
VERSION     DBNGVD010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4015638"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC16CM_01280"
     CDS             <1..494
                     /locus_tag="AC16CM_01280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138488.1"
                     /translation="HERPGDVPAIREVNRKAFGREEEAWLVDELRDGDYTRLSLVAEE
                     ARRIIGHILFSEAVIRTEGGDTEALALGPVAVTPELQGQGVGAALVREGLDRCARLGS
                     RVIVLLGHPDYYPRFGFSAKLARGLSSPYSGDAFMALELVPGALSGVSGEFEFAPPFG
                     ALS"
     gene            491..1417
                     /locus_tag="AC16CM_01285"
     CDS             491..1417
                     /locus_tag="AC16CM_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HIR3138489.1"
                     /translation="MTRVLDARETADLLPYPDLADSIREMALARDSEDVQAPPRFALP
                     LREGGVLLVMPASDQDIAITKLATVHPENPGRGLPTIQGEVVVMDATTGERLGVLEGS
                     VVTARRTAALSMLAARELAQHPEGPLLVIGAGTQGRSHLEAFRAGLGVRRAFIASRSR
                     ENAVSLAGHARSLGMEAEAVEGPGEVLDDIGLIVTATTSKEPVLPEEIPDGVFVTAVG
                     AFEPEAAELPPTLVSTSEIFVDTLQGAKEEAGDLIQAEHAGAFAWDDATELEAVLRPP
                     RRRHDEGVVVFKSVGHALWDLAAARTAFVNRA"
     gene            1614..1994
                     /locus_tag="AC16CM_01290"
     CDS             1614..1994
                     /locus_tag="AC16CM_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3138490.1"
                     /translation="MQKVLVVSEPLSDIEVGRESEEMCTLAKMFNGFANSTRLSILLL
                     LVRRGEMKVGELVDELGAPQPRVSDHLRCLAWCGYVQVRREGRNAYYSVSDERVMRML
                     ELGEELLQDNVEYLEACDDIDQGC"
     gene            complement(2022..2759)
                     /locus_tag="AC16CM_01295"
     CDS             complement(2022..2759)
                     /locus_tag="AC16CM_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR3138491.1"
                     /translation="MDEAGQEGAVDADRYEVLSFDCYGTLVDWEAGIVSGLRPVLRAH
                     GVEASDEEILDLHAGTEHRLQSASERGGYVRYRNVLGEEVREAGRRWGFEPSPSEVEA
                     LADSVRYWLPFPDTVNALRALKSRYRLAIISNVDDGLFALTARYLEVEFDYIITAEQA
                     GTYKPALGNFELALERMGAAPERVLHVAESLFHDHVPARKLGLDTAWVHRRAAKGGFG
                     ATPPAEASFDIEVPDLESLVFALGLRR"
     gene            2885..3679
                     /locus_tag="AC16CM_01300"
     CDS             2885..3679
                     /locus_tag="AC16CM_01300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138492.1"
                     /translation="MAASIMKPYMFDETSALEYQRLDLMSRILDPWTRGYLDAIGIGP
                     GWRCLELGGGNGSVSEWLCDRVGPSGSVTAVDINPVLLELIPAQNLTVERRDVRSDDL
                     PEGAYDLVSCRALLHQISDYAPAVLGRMAAALKPGGWLLVQEPDFHLAGTTEPEVWAN
                     TWRSLIEWGHDNGVDWLIGRELPGMVGSLGIGQPRAETNVQNIRGRDRGALYFRLFFD
                     EVRDRVLGSGHLDATTLDAATVLLDDPDYWTQCWMMTAVWARKQPA"
BASE COUNT          697 a         1239 c         1256 g          657 t
ORIGIN      
        1 ggcatgagcg accgggcgac gtgccggcta tcagggaagt caacaggaag gcgttcggac
       61 gcgaagagga ggcttggctt gtcgacgaat tgcgcgacgg ggactacacg aggttgtcgc
      121 tggtcgccga ggaagctaga cgaatcatcg gccacattct attcagcgaa gccgtgatcc
      181 ggacggaggg cggcgacacg gaagccctgg ccctcggacc cgtggccgtg acgccggaac
      241 ttcagggaca gggagtaggc gccgcgctgg taagagaggg tctcgaccgc tgcgcgcgcc
      301 tggggagccg cgtgatcgtg cttctcggcc accctgacta ctacccgcgc ttcggtttct
      361 cagcgaaact ggccagaggc ctgtcatcgc cctactccgg agacgctttc atggctctgg
      421 aactggtccc cggggccctc agcggggtgt ccggagagtt cgagttcgcc cctccgttcg
      481 gggcactctc gtgacccggg tcttggacgc gcgagagacc gcggatctcc taccctaccc
      541 cgacctggca gactctatcc gcgagatggc gctcgccagg gactcggaag acgtgcaagc
      601 cccgccccgc ttcgcgcttc ccctccggga aggcggcgtc ctgctggtca tgcctgcttc
      661 cgaccaggac atcgcaatca ccaagctggc aacggtccac ccggagaacc cggggcgtgg
      721 cctgccgacc atacagggtg aggtcgtggt gatggacgcg acgacgggcg agcggctcgg
      781 cgtcctcgaa ggcagcgtgg tcaccgcgag gcgtaccgca gccctctcca tgctagccgc
      841 ccgcgagctc gcccaacacc ccgaaggtcc cttgctcgtc atcggcgcgg gcacccaggg
      901 acgctcccac ctggaggcgt tccgcgccgg actcggcgtc cgccgggcct tcatcgcctc
      961 ccgaagccgg gagaacgccg tctccctggc cggtcacgcc agaagcctcg gcatggaggc
     1021 ggaggcggtc gagggaccag gtgaagtgct cgacgacatc ggtctcatcg tcacggcgac
     1081 cacgagcaag gagcccgttc tgccggaaga gattccggat ggcgtcttcg tgacggcggt
     1141 cggagccttc gagccggagg cggccgagtt acctccgacc ctggtctcca cctcggagat
     1201 cttcgtggat acgctgcaag gcgcaaagga ggaagcggga gacctgatcc aggccgagca
     1261 cgccggggct ttcgcgtggg atgatgcaac ggagctggaa gcggtactcc gcccgccgcg
     1321 aagacgccac gatgagggcg tcgtcgtgtt caagagcgtt ggccacgccc tctgggacct
     1381 cgcggccgca aggacggcct tcgtaaaccg ggcctgagcc ggtggaccgc gtaacatcag
     1441 ccagaaagct gaccactgaa agctgatagc tgacagctcc taaaggcagt cggcgagcct
     1501 ttcagacggc cctgcacggt gacggaacat ccgattctga ccgtccagaa tgtaatatgg
     1561 tacacacttt ttggtgatcg gtgtatagta ggaatgtacg gaaggagatc ttggtgcaaa
     1621 aagtacttgt cgtgtcagaa cctctatccg atatcgaggt agggcgcgag agcgaggaga
     1681 tgtgcaccct cgcgaaaatg ttcaacggtt tcgctaactc gacgaggctt tcgatcctgc
     1741 tgctcctggt gcggcggggc gagatgaagg tcggggagct ggttgatgag ctcggcgcgc
     1801 cgcagccgcg ggtctccgac catctccgtt gcctggcctg gtgcggctac gtgcaggtac
     1861 gccgcgaggg ccgaaacgcc tactactcgg tgtcagacga acgcgtcatg cgcatgctcg
     1921 agctcggcga agagctcctg caggacaacg tcgaatacct cgaagcctgc gatgatatag
     1981 accagggctg ctgatccaga accccggccc atcgtcagat cctatcgcct gagccctagg
     2041 gcgaagacca gcgactcgag gtcgggcacc tcgatgtcga aggaggcctc cgccggtggg
     2101 gtcgccccga atcctccttt cgccgccctc cgatggaccc aggcggtgtc cagccccagt
     2161 ttcctggccg gaacatggtc gtgaaacagg ctctccgcga cgtgcagtac cctctcgggg
     2221 gccgctccca tcctttcgag cgccagttcg aagttgccca acgcgggttt gtaggtgccg
     2281 gcctgctcgg ccgtgatgat gtaatcgaac tcgacctcca ggtacctggc cgtcagggcg
     2341 aacagcccgt cgtccacgtt ggagataatc gccagccggt acctgctctt gagggcgcgc
     2401 agcgcgttca ccgtgtcggg gaaaggcagc cagtacctca cggagtcggc gagcgcttcg
     2461 acctcggagg gactcggctc gaaaccccac ctcctgcccg cctcgcgaac ctcctcgcca
     2521 agcacgttgc ggtatctgac gtagccgccc ctctccgagg cagactgcaa cctgtgctcc
     2581 gtcccggcgt gcaggtccag tatctcctcg tccgaggctt cgacgccgtg ggcgcgtagc
     2641 acgggcctga gcccggagac gatcccagcc tcccagtcca ccagcgttcc gtagcaatcg
     2701 aaagacaaga cctcgtacct gtccgcatcc acagcaccct cctgtccagc ttcatccatc
     2761 ccgaaccctc acgacatcaa ggttagcaaa gccccaaccc tccatggcac agcgcgaact
     2821 cggctgcgag ggtggtgccg tgctcttccg tccgctatcc tcaaatattc agtagcttcg
     2881 agagatggcg gccagcatca tgaaacctta catgttcgac gagacttcgg ccctggagta
     2941 ccagcggctc gacctaatgt ccaggatcct cgacccctgg acccgagggt acctcgatgc
     3001 tatcgggatc ggtccaggct ggcggtgcct ggagttgggc ggcggcaacg gcagcgtctc
     3061 ggagtggctc tgtgacaggg tcgggccatc gggaagcgtg acggccgtcg acatcaaccc
     3121 ggtgctgctc gagttgatcc ccgcgcagaa cctcacagta gagcggaggg acgtgcgcag
     3181 cgatgacctg ccggagggcg cctacgacct ggtaagctgc cgcgccctgc tgcaccagat
     3241 ctccgactac gccccggcgg tgctcgggcg catggccgcc gccctgaaac cgggcgggtg
     3301 gcttctggtc caggagccag acttccacct cgctgggacc acggagccgg aagtgtgggc
     3361 caacacctgg agatccctga tcgagtgggg ccacgacaac ggggtcgatt ggcttatcgg
     3421 ccgtgagttg cccggcatgg tcggctcgct tgggatcggg caaccgcggg ccgagacaaa
     3481 cgttcagaac atccgtggcc gggatcgcgg ggcgttgtac ttccggctgt tcttcgacga
     3541 ggtgcgggac agggtgctcg gcagcggtca cctcgatgca acgacgctcg acgccgccac
     3601 ggtgctgctc gatgatccgg actactggac gcagtgctgg atgatgacgg ccgtctgggc
     3661 ccgcaagcag ccggcctgag cgcaggcggc aggcccgctg catccgacca cggtgcgaac
     3721 atcgctccgg atgcgggttc gcgggtgcga attcgtgttt acgtggccgg taggtagggg
     3781 acctagatag ggccgttcta taccacacac taacgagagg tgtttcaggc atggcccagg
     3841 cacaggaac
//
LOCUS       DBNGVD010000049         4085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1647859, whole genome shotgun sequence.
ACCESSION   DBNGVD010000049 DBNGVD010000000
VERSION     DBNGVD010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1647859"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC16CM_01305"
     CDS             complement(<1..359)
                     /locus_tag="AC16CM_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3138493.1"
                     /translation="MSEKWYPRRGPHVPISLSMEDHRPMYRQIESQLRDFILSGALRP
                     GTKLPTIRALARDASCSVITTQRAYRDLEEEGLILTRQGRGTVVAEITEEKLRARRHE
                     AVKAAFAEAVGTGRRAGL"
     gene            complement(356..1192)
                     /locus_tag="AC16CM_01310"
     CDS             complement(356..1192)
                     /locus_tag="AC16CM_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138494.1"
                     /translation="MGVGSRGSGGGLDLFGAVNPSSRTSGMRMCAQSFWLARREMKHA
                     WPSYVSNASIAMVLGSVAAVSLSFGLSGFEARILRGPGMEAFYGAFLSDYLFLLVCAL
                     LGTNGVLIGYYARNRRSTFTRKLRPLRNIPVSAAVLVGSRALGALFALFVGALAFFVP
                     VFFLSDLGEDLGVKTYLYFSAVWVGYGLLGSGISLYFEFGSSGRVYRLISYGFAVPLM
                     VAVVLLEISGYAGLVGRVAGMSQGGYGALLAGLSVLLGGAAFLLLSRATVHRLLTREL
                     PA"
     gene            1566..2855
                     /locus_tag="AC16CM_01315"
     CDS             1566..2855
                     /locus_tag="AC16CM_01315"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-tagatose-bisphosphate aldolase, class II,
                     non-catalytic subunit"
                     /protein_id="HIR3138495.1"
                     /translation="MSGLLLDTVERHRSGEAVGVYSVCSAHPLVIEASVMQVLGEDGY
                     LLVEATSNQVDQFGGYTGMKPLDFRALVLGLAERRGLPGDRVVLGGDHLGPNRWRGLP
                     AEEAMGHAGDLVALYVASGFTKIHLDCSMACTDDTEPLDDEVVAERAATLAARAEGEA
                     SGDEIVYVVGTEVPVPGGAHEALGKLTPTSAGAARATLAAHEEAFRARGLDGAWERVV
                     ALVVQPGVEFDLERVIDYEESATEELRRVLEDRGRLIFEAHSTDYQTRANLAALVRNH
                     WAVLKVGPALTFAMREALFALAAIEDELVAEEERSELVSVVDRRMREEPGYWEGYYQG
                     GPEAQRLLRRYSYSDRIRYYWSHPDVEGARKRLFTNLAHQKIPQTLLSQYLPLQYERV
                     RLGRLDCAPEALVFDRVRDTLEDYSAACNQTTREARL"
     gene            2852..4021
                     /locus_tag="AC16CM_01320"
     CDS             2852..4021
                     /locus_tag="AC16CM_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3138496.1"
                     /translation="MNTDDNLLASLSAGQGGETTAREIAQQPEVWRETEQVVASRRED
                     VDAFLRPLMDRPDLRIILTGAGTSAFAGEILAPALSRRTGRRVDAVATTDIVSSPKEY
                     FANDGPTLLVSFARSGNSPESVAATELADRLLPRCHHLILTCDRDGRLYEKHADEEGS
                     FVLLMPEATNDQGFAMTSSFTSMMLAAWVILGRVEDPGELAGRLATAAEKLLDAREED
                     LRTLASSGYQRVVFLGSGPLRGLAHESSLKLLELTAGGIVTYFESPLGFRHGPKSILD
                     DRTLAVLYVSGDEHTRKYDLDLLEELKNSMPSENVVAVSAQEPGAEDPGFWLVEGVDG
                     VDDAMLALPFVLVAQLLGIFFSLELGYTPDNPFPTQEVNRVVQGVSIHPIEEGSS"
BASE COUNT          754 a         1280 c         1365 g          686 t
ORIGIN      
        1 agacccgcgc ggcggccggt cccgacggcc tcagcgaagg ctgcctttac ggcctcgtgg
       61 cgcctggctc tcaacttctc ctcggtaatc tccgcgacca cggtgcccct cccctgcctg
      121 gtcaggataa gaccctcctc ctccagatcc cggtaggcgc gctgcgtcgt gatgacgctg
      181 caagaggcgt ctctggcgag cgccctgatg gtgggcagct tcgttccggg cctgagcgcg
      241 cccgagagga tgaagtccct caactgcgac tcgatctgcc tgtacatggg cctgtgatcc
      301 tccatggaga ggcttatggg gacgtgcggc ccccgccgcg gataccactt ctcgctcacg
      361 cagggagctc tctggtaagc aagcgatgaa ccgtggccct cgacaagagc agaaaggctg
      421 cgccacccag caggaccgag aggccggcca gaagcgcccc gtacccgcct tgtgacatcc
      481 cggccaccct tccgacgagg cccgcgtatc ccgatatttc gagcagcacg accgcgacca
      541 tgaggggaac ggcaaagccg taggagatga gcctgtagac cctaccgctc gagccgaact
      601 cgaagtacag cgagatcccc gatccgagca ggccgtaccc tacccagacc gcgctgaagt
      661 acaggtacgt cttcaccccg agatcttctc cgaggtccga aaggaagaac accggcacga
      721 agaaggcaag cgcgcctacg aagagcgcga acagcgcgcc gagcgcgcgg ctacctacga
      781 ggaccgccgc agagaccggt atgttcctca agggccggag cttgcgagta aacgtgctac
      841 gccggtttcg cgcgtagtac ccgatcagta ctccgttcgt cccgagaaga gcacacacga
      901 gcaggaagag gtagtccgag aggaaggccc cgtagaacgc ttccatcccg ggcccccgca
      961 atatcctggc ttcgaacccg gagaggccga aggacaagct taccgcggcg acggatccaa
     1021 ggaccatggc gatcgaggcg ttcgacacgt aagagggcca cgcatgcttc atctctcttc
     1081 ttgcgagcca gaacgactgc gcacacatgc gcatgccaga ggtcctgctc gacgggttga
     1141 ccgcgccgaa cagatccagc ccccctccac ttcctctcga ccccacaccc atctcgcctc
     1201 ccaagacaca tgtttgctta tataactata tacaatatag aggcgttgac cattatctgt
     1261 caagagatcc ggaggatttt tcggtcgggt gtctgctcgc ccgtgcgtgt ttgaggtcgc
     1321 gagacacaat aatccgcgcg tatttgatct tctttgaagg tttatgatct ggtcggggag
     1381 ggaacggttc aggcgaggtc cgttcgcttt ggtgtacggg ccgccactag cagagagcgg
     1441 ggggcgaggg tgatctggta ctcagttggc cgttcgcggg gtggacgata cagggatggt
     1501 cgttggcccg aggcttcgtg gcccgtttgc atgagggctg gcgtgcgtgc gggaggtagg
     1561 ccctcgtgag cgggctgttg ttggacacgg tggagaggca caggtcaggt gaggccgtgg
     1621 gcgtgtactc cgtctgctcc gcgcacccgc tggtcataga ggcttcggtt atgcaggtcc
     1681 tcggcgagga tgggtatctc ctggtggagg ccacgtccaa ccaggtagac cagttcggag
     1741 gttacacggg gatgaagccc ctggatttcc gcgcgctcgt gctgggcctg gcggagcggc
     1801 gggggctacc cggggaccgt gtcgtgctgg gtggggacca tctcggccca aaccgctgga
     1861 ggggattacc ggccgaggag gcgatggggc acgcggggga tctcgtcgcg ctctacgtgg
     1921 cttccggctt cacaaagata cacctcgact gcagcatggc ctgcacggac gacacggagc
     1981 cgctggatga cgaggtcgtc gcagagaggg ccgccacgct cgccgccagg gccgagggag
     2041 aggcgtcggg cgatgagatc gtctacgtcg tggggacgga ggtgcccgtg cccggcgggg
     2101 cccacgaggc gttgggaaag ctcaccccaa cctccgcagg cgcggcgcgc gccacgctgg
     2161 ccgcgcacga ggaagctttc cgggcaagag ggctagacgg tgcctgggag cgggtggtgg
     2221 ccctcgtcgt acagcccggt gtggagttcg acctggagcg cgttatcgac tacgaggaat
     2281 ccgcaacgga ggagctcagg cgcgtgctgg aagaccgcgg ccggctcatc ttcgaggccc
     2341 actccacgga ctaccagacg cgggccaacc tcgcggcgct cgtgaggaac cactgggcgg
     2401 tgctcaaggt ggggccagcg ctcacgttcg ccatgcggga ggcgctgttc gcgctcgccg
     2461 cgatcgagga cgagcttgtg gccgaagagg agcgttctga gctggtctcc gtcgtcgacc
     2521 gccgcatgcg ggaggagcca ggttactggg aaggctacta ccagggcgga ccagaggccc
     2581 aacgcctcct gcgtcggtac agctacagcg acaggatccg ttactactgg tcgcatcccg
     2641 acgtcgaggg cgccaggaag cgtctgttta cgaacctggc ccatcagaag ataccccaga
     2701 ccctgctcag ccagtacctg ccgctccagt acgaacgcgt gcgcctgggc cggttggact
     2761 gcgcgccaga agccctcgtc ttcgaccgcg tacgcgacac gctggaagac tacagcgccg
     2821 catgcaacca gaccacaagg gaggcccgac tttgaacaca gacgacaacc tactcgcaag
     2881 cctgagcgcc ggccagggtg gggagacgac ggcgcgtgag atcgcccagc aaccagaggt
     2941 ctggcgtgag acggagcagg tggttgcctc acggcgggag gacgtcgatg cgtttcttcg
     3001 gccgctcatg gaccggccgg accttaggat catcctcacc ggcgcaggca cgtcggcctt
     3061 cgcgggggag atactggccc ccgcgctctc cagacggacg gggcgccgcg tggacgccgt
     3121 cgcgaccacg gatatcgtct ccagcccgaa ggagtacttc gcaaacgacg ggccaacgct
     3181 gctggtctcg ttcgcgcgct cgggcaacag cccggagagc gtggctgcga ccgagctcgc
     3241 agaccgcctg cttccgcggt gccaccacct gatcctgacc tgcgacaggg acggcaggct
     3301 ctacgaaaag cacgcggacg aggaaggatc cttcgttctc ttgatgccgg aggccaccaa
     3361 cgatcagggc ttcgccatga cgtcgagctt cacgtcgatg atgctcgcgg cctgggtcat
     3421 cctggggagg gtagaagatc caggcgagct ggctgggcgt ctcgccacgg ccgctgagaa
     3481 gttgctggat gcccgtgaag aggacctaag gacgttggca tcctcgggct accagcgggt
     3541 cgtcttcctc ggtagcggac cgctaagggg gctcgcccac gagtcttctc tcaagctcct
     3601 cgagctgacc gcgggcggga tagtaacgta cttcgagtct ccgctcggct tcaggcacgg
     3661 gccgaagtcc atcctcgacg acaggacgct cgccgtcctc tacgtctcgg gcgacgagca
     3721 cacgcgaaag tacgatctgg accttctgga agagctcaag aactcgatgc cttcagaaaa
     3781 cgtcgttgcg gtctcggcgc aggagccggg ggcggaagat ccgggcttct ggctcgtgga
     3841 gggcgtggac ggggtggatg atgcgatgct ggcgctgccg ttcgtcctgg tcgcccagct
     3901 cctcggcata ttcttctcgc tagagctcgg ctacacgccg gacaacccgt tcccaaccca
     3961 ggaggtgaac cgcgtggtac agggcgtctc cattcatccc atagaggagg ggagctcctg
     4021 atgtacctcg gcgtcgatgg gggaggcacc aagacggccc tttgcctcct tcgcggggac
     4081 gggcg
//
LOCUS       DBNGVD010000050         3434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4562465, whole genome shotgun sequence.
ACCESSION   DBNGVD010000050 DBNGVD010000000
VERSION     DBNGVD010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4562465"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..115
                     /locus_tag="AC16CM_01325"
     tRNA            42..115
                     /locus_tag="AC16CM_01325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:cgg)
     gene            complement(271..1341)
                     /locus_tag="AC16CM_01330"
     CDS             complement(271..1341)
                     /locus_tag="AC16CM_01330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3138497.1"
                     /translation="MRTLRRGDRGREVVDLQTRLQALGFDLGNRGVDGVFRGDTELAV
                     RSFQQGLGLLADGLVGPITWREIVEADYRPGGRLLYLRQPPFRGADVVELQQMLDDLG
                     FDPGSVNGLFDMRTVRAVRDFQKNAGLRDDGVVDASVFKVLNTFAAQSLGTHQLPDKS
                     GGYFNDDLFSGAIVVDAAHGGRDTGYQSPTGLSEADLNLAVAQELAQILPAREVLLTR
                     SGDEEVSAADRAFLANSSGARMIVSIHHAAHRTPTANGTSSFYFERLGYQSHRGHMAA
                     AYLQRGVSQALGTSDIGEFGRSFEILRETNIPAVMIEPLYLTNPQELDLASDPYYPAT
                     VAEAVAGALEQYAGRDAAFIAV"
     gene            complement(1446..1715)
                     /gene="groES"
                     /locus_tag="AC16CM_01335"
     CDS             complement(1446..1715)
                     /gene="groES"
                     /locus_tag="AC16CM_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3138498.1"
                     /translation="MKFKPLGERALVQIVEREETTASGIVLPDTAKEKPQTASVVAVG
                     QFENGVKVSEGDVIVFAKYSGTEISLDGEDYMILDADDILGIVES"
     gene            complement(1828..2139)
                     /gene="trxA"
                     /locus_tag="AC16CM_01340"
     CDS             complement(1828..2139)
                     /gene="trxA"
                     /locus_tag="AC16CM_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3138499.1"
                     /translation="MAVAEVTDATFESDVLRSERPVIVDFWAPWCVPCRRISPILEEM
                     SESRDDVTFVKVNVDDNPSTAMNYSISSIPTIARFEEGQVTQQAVGALPKNQLSAQLG
                     L"
     gene            complement(2297..3256)
                     /locus_tag="AC16CM_01345"
     CDS             complement(2297..3256)
                     /locus_tag="AC16CM_01345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR3138500.1"
                     /translation="MILTITLNAAVARTLVVPSLTVGHRHRAPESRTLAGGKGVNVAR
                     SLRTLDVPVLAAGFAGGRNGDAIRDGLSEAAIPFDLVEIEGHSRTSTAIIDPMAGTQT
                     EINEYGPEVTQAEAKEFSRRLEHLMEYATAVVFAGSVPPNLDESFLVDLVRRARERGL
                     FTVVDSPSSVLRAALKANPSLVSPNQSEAETVAGFDFIEEGDFPLGLSRLVELGAAHA
                     CITSPEGLSYLSIEDGAISARAPRVETVSTVGSGDAYLAGLLAGLLHRNLAPAEAVRL
                     AAGCAAANAETLGAGLFEARRAEELADSVQVQVYSRSLEESRT"
BASE COUNT          552 a         1128 c         1098 g          656 t
ORIGIN      
        1 gctgacaggt gcggtccggc tggtataatg tccccttcag tcgggacgta gcgcagcctg
       61 gtagcgcgcc tcgttcggga cgaggaggtc ggaggttcaa atcctctcgt cccgactcct
      121 cagcacggcg cttcgcttcc cctcagcatg ctcatgcttg cgccttggct tgtcggcacg
      181 cttcgagcct tcggctcttg cttccagctt atggtaagta cggtaagtcc ttgtatttgt
      241 acctgaaacc tgtggcctga agcctatagc ctaaacagct atgaaagcgg cgtcgcgtcc
      301 agcgtactgt tccagggcgc cggccacggc ttctgcgacg gtggccgggt agtatgggtc
      361 agaggcgagg tctagctcct gcgggttggt caggtagagg ggttcgatca tcacggccgg
      421 gatgttcgtt tcgcgcagga tctcgaacga ccttccgaac tcgccgatat cgctggttcc
      481 aagtgcctgg ctgacaccgc gctgcaaata tgcggctgcc atgtggcccc tgtgcgactg
      541 gtagccgagc cgttcgaagt agaaggagga tgtgccgttg gcggtcgggg tccggtgggc
      601 ggcgtggtgg atagagacga tcatgcgcgc ccccgatgag ttggcgagaa aggccctgtc
      661 ggccgctgag acctcctcgt ccccgctcct ggtcaggaga acttccctgg ctggcaggat
      721 ctgggcgagc tcctgggcca cggcgaggtt caggtcagcc tccgagagcc ccgtcgggct
      781 ctggtacccc gtgtccctac cgccgtgcgc ggcgtctacc acgatggccc ccgagaagag
      841 gtcgtcgttg aagtagccgc cgctcttgtc cgggagctgg tgggtaccga gcgattgggc
      901 cgcgaaagtg ttcagaacct tgaagaccga ggcgtctacc acgccgtcgt ccctcaggcc
      961 cgcgttcttc tggaagtcgc ggacggccct cacggtacgc atgtcgaaga ggccgttcac
     1021 ggagcccggg tcgaacccga ggtcgtcgag catctgctgc agctcgacca cgtcggcacc
     1081 cctgaaaggg ggctgcctca ggtagagcag gcgccccccc ggcctgtaat cagcctccac
     1141 gatctcacgc caggttatgg ggccgaccag cccgtcggcg agcagcccga ggccctgctg
     1201 gaaggatctg accgccagct ccgtgtcgcc cctgaagacg ccgtccacgc ccctgtttcc
     1261 aaggtcgaag ccgagggcct gcaggcgggt ctggagatcg acgacctcgc gtcctctgtc
     1321 tcccctacga agcgtgcgca cgtgccgaac cgttctctcc ctggcgtcca gactactcta
     1381 ccggccaaaa caggagcgcc gggcgtacgc ccggcgctcc ggaaggccga tcctcgctcg
     1441 accctctagc tctcgacgat ccccaggatg tcgtcggcgt cgaggatcat gtagtcctcg
     1501 ccgtcgaggc tgatctcggt cccggagtac ttggcgaaga cgataacgtc gccctcgctg
     1561 accttgaccc cgttctcgaa ctgcccgacc gctacgaccg aggccgtctg cggcttctcc
     1621 ttggccgtgt cgggtagcac gatacccgag gcggtggtct cctcgcgctc gacgatctgg
     1681 acgagcgcgc gctctcccaa aggcttgaac ttcatctgct tttacctcct gattgctctt
     1741 acttctttac gacaccaaga ccggtcatac ccggtcttgc gagccttacg cgccagagat
     1801 tgtaaccgcg agtgttgcta ttcgcaatta caggccgagc tgcgccgaga gctgattctt
     1861 gggcagggct cccacggcct gctgggttac ctgcccctcc tcgaagcggg cgatggtcgg
     1921 gatgctggag atgctgtagt tcatggccgt cgaggggtta tcgtccacgt tgaccttgac
     1981 gaacgtcaca tcgtcgcgcg actctgacat ctcttccagg atcggggaga tgcgccggca
     2041 cggcacgcac cagggcgccc agaaatctac tattaccggt ctctccgagc gcagaacgtc
     2101 gctctcgaaa gtcgcatccg ttacttcggc taccgccatc tctcgaacct ccgttggtct
     2161 ctcgcgagcg gttttcgcgc aacattctag cacgcacggg ggagctatca gctatcagct
     2221 atcagccttc atcaagaact aaaagccgac ggctgacggc tgatggctga cggcgcggtt
     2281 cgcagggcga atcgcttcag gtgcggctct cttccaggct gcgggagtac acctggacct
     2341 gcacggagtc ggccagctct tcggcgcggc gcgcttcgaa gaggccggcg ccgagggtct
     2401 cggcgttggc ggcggcacat cccgcggcga ggcggacggc ctcggcgggc gccaggttgc
     2461 ggtgcaggag gccggcgagg aggccggcga ggtaggcgtc gccgctcccc accgtggaga
     2521 cggtctcgac cctcggggcg cgcgcagaga tagccccgtc ctcgatgctg aggtaggaga
     2581 gcccctccgg agaggtgatg caggcgtggg ctgcaccgag ctcgacgagg cgggagaggc
     2641 cgagggggaa atctccctcc tcgataaagt cgaagcccgc cacggtctcg gcctcgctct
     2701 ggttggggct aacgagcgag gggttcgcct tgagcgcggc cctcagcacg gacgagggcg
     2761 agtcgaccac cgtaaagagc ccgcgctccc tggcgcgacg cacgaggtct acgaggaagc
     2821 tctcgtccag gttcggtggc acgctgcctg cgaagaccac ggcggtcgcg tactccatga
     2881 gatgctccag gcgccgggag aactccttgg cctcggcctg ggtcacctcc ggcccgtatt
     2941 cgttgatctc ggtctgcgtc ccggccatcg gatcgatgat tgccgtcgag gtgcgcgagt
     3001 gtccttctat ctccacgagg tcgaacggta tggccgcctc ggagagaccg tcccttatgg
     3061 catccccgtt gcgcccaccg gcgaacccgg ccgcaagaac gggcacgtcc agcgtgcgta
     3121 aggaccttgc gacgtttacg cccttgccgc ctgcgagcgt cctgctctcg ggtgccctgt
     3181 ggcggtggcc cacggtcagg cttggaacta ccagcgttcg ggccacggcg gcgttgaggg
     3241 tgatggtcag tatcatgact ctccctagcc tatcctctac ctaaaaatcc ctcccacggt
     3301 ataccacagg cgctgcccga gcgaaggctt ctcgtagcca tgtctggcga ccagcgcggt
     3361 ctcccccact ctctccccgt cgaccctcac gacgacccgg cccagcgtgg tccctgccct
     3421 ggccgcgagc ggtg
//
LOCUS       DBNGVD010000051         6316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_7898, whole genome shotgun sequence.
ACCESSION   DBNGVD010000051 DBNGVD010000000
VERSION     DBNGVD010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_7898"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..583
                     /locus_tag="AC16CM_01350"
     CDS             203..583
                     /locus_tag="AC16CM_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3138501.1"
                     /translation="MQYVHTCYRVLDLDKSIDFYENKLGLELARKSPVGDDATNAFFA
                     VPGDPEPRLELTLNHDQDEPYELGTGYSHVAFAVEDLDALAERLEEAGGVEFESKPHA
                     MSSGTRIFFVKDPDGYRIEFIERA"
     gene            741..1385
                     /locus_tag="AC16CM_01355"
     CDS             741..1385
                     /locus_tag="AC16CM_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR3138502.1"
                     /translation="MASARRKDFGDCEDEGGYTLPEVLTAVAIMSVILAIGVIVLLAL
                     LERWRVEAAADQFAADLRLVHTRATEQLTDWRVVFMDDGTPLVGCSAADYCLVKLSLP
                     YGAGDPAPALDGSVRPAVRELPEGTRIKEVTFDPDCSHGDRNAAVPPSTCGATATQAG
                     ATRTLEFNPNGTVRTLRPGQSGTVVISSDDGSPVCPVVFNAPTSRIRIGEIYYP"
     gene            1366..1761
                     /locus_tag="AC16CM_01360"
     CDS             1366..1761
                     /locus_tag="AC16CM_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3138503.1"
                     /translation="MRSTTRRTAFDPGYSSGEVMVAIVVLTVAIVPMAGMFGAAIEAA
                     DAGGEYDRARSCAVQRLEQIKSLPYESVEAGLPDGVCEPIGLRYDVATYPVGTDLATG
                     PGEAGLTMVTVTVDSDGISYGISGVVSRW"
     gene            1758..2372
                     /locus_tag="AC16CM_01365"
     CDS             1758..2372
                     /locus_tag="AC16CM_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR3138504.1"
                     /translation="MRRHGQAGFTLVEVLVAASMMFAVLFALYAVFDVSVRAFGYGGD
                     RVEAVANARLVMDRMTREIRVAYPYDPTADPPRRHLFLNPLDPTEPAVPTATRLAFGN
                     ETTGDRKIGASEVIGYYSSGQDLKRSKGGSVQTLLDTVATNGLRLTPCRTAEDCPPAV
                     ALTDEAAVRLLRIELTVEVQRRGEDPARQRLATDVYLRNRVVPR"
     gene            2402..2782
                     /locus_tag="AC16CM_01370"
     CDS             2402..2782
                     /locus_tag="AC16CM_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138505.1"
                     /translation="MVLGLTIIIVLVVGVMGAGLLTFVSADLQSIVAVNKGEQALQLA
                     EAGIEVAKSHLANDPGPTDWSSGELHAQGVDANSVTVTIDQNAGSFEVVSTGQYGETK
                     RKVEAVFEVQDGDPRLIGWRELYE"
     gene            2905..3162
                     /locus_tag="AC16CM_01375"
     CDS             2905..3162
                     /locus_tag="AC16CM_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 RNA polymerase sigma factor region 4
                     domain-containing protein"
                     /protein_id="HIR3138506.1"
                     /translation="MDESVGTRVGGPEDPRAGLEAVVALRRTLEALEAAQVENAFVAG
                     WSWARIAEVLGVSRQAVHKKHAARIRARYPGRPPGGKEGGG"
     gene            3159..3824
                     /locus_tag="AC16CM_01380"
     CDS             3159..3824
                     /locus_tag="AC16CM_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3138507.1"
                     /translation="MIFHRFTGEARGSVEAALEEARMLGHDSLGDEDLLLGILRAGEG
                     VAAEALESFGVTLEDARGESEAMLSGALASIGISLEEIRKEAGDAFDLRLPEKRRIPF
                     APGAKKVLERALARAVRLGDRYLGTEHILLAILENAEGTAVRMLARLGVAPEALEERL
                     FELRGRAAGSRGVLDSPVSVNRPRSFAVRNARMRLWRREKNAVGQSGSGWSGAASAGR
                     WGC"
     gene            3743..4177
                     /locus_tag="AC16CM_01385"
     CDS             3743..4177
                     /locus_tag="AC16CM_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138508.1"
                     /translation="MEEREERGGAVRVGMVWGGIGGAVGLLVSLLGSLIGIVVAVFIG
                     ISCGKRAAAAGPWAGAVSGLVAGAIVAPVYALGSTIGALFAAQEIGAARLAQTLSELL
                     GSNVSADEAWVFMLISLVLSAVIEIILLTGVAAAAGAWAKRG"
     gene            complement(4205..4681)
                     /locus_tag="AC16CM_01390"
     CDS             complement(4205..4681)
                     /locus_tag="AC16CM_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR3138509.1"
                     /translation="MDRQLQIQVLLDHYEKPRHRGALEDADVTMPGGNPGCGDVVTVY
                     FKGAEDQEHIGDATYEGEGCTISMAASSMILEEVHRGNLTMEQVLDMDYNEMIEQLGR
                     QVVASRPRCATLGLGTLKAAIRKYQKDRRLDEAGVERPDEGGLVFGDTAAEAARTE"
     gene            4785..5867
                     /gene="ychF"
                     /locus_tag="AC16CM_01395"
     CDS             4785..5867
                     /gene="ychF"
                     /locus_tag="AC16CM_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3138510.1"
                     /translation="MRVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVAAV
                     PDRRLDALAKTMKSKRVVPATVDFVDIAGLVRGASSGEGLGNQFLGHIRECDAIAHVV
                     RCFEDENVVHVHGGVDPEGDIETIDTELLLADLATVERRLERIGRAAKGGDAALRAEA
                     DELERLRDHLSDGSPARAYPHSETLAEAMDALITAKPTLYVANVDEESVAEGNTYSSR
                     VEKLAAREGAEAVRISARLEAEVVDLPEEEATEYLQMLGVETTGFEEFVRSAYSLLGL
                     VTFFTAGEKESRAWTVSQGSTVRQAAGRIHTDMERGFIAAEVGGWQDIVNAASWAKAR
                     EEAKVRLEGRDYLIKDGDTVLIRFNV"
     gene            5873..6268
                     /locus_tag="AC16CM_01400"
     CDS             5873..6268
                     /locus_tag="AC16CM_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR3138511.1"
                     /translation="MPPFVRPGRPLARSRLALVTTGGVHLPDQRRFDIDDFEGDCSYR
                     EVPADAEELTWTHAYFRPDEGSDLDAVFPLETLRQLAEEGLVGGLGPRHFSFMGAIHD
                     PTPLEEETAPEVARKLIEDGVDAVLLTPS"
BASE COUNT         1096 a         1950 c         2192 g         1078 t
ORIGIN      
        1 ggccgcagag cgatctggct gaaagcgagc caggcggagc cgggcgaagc gttgccgaca
       61 agcgaaagcc gctaggctgg agcgtgctga caagccgcga agcggcgtgc tgaaagcgta
      121 ggcgaagccg tagcgtgctg acaagcgacc gaagggagcg tgctaatatg ccaggagatc
      181 tggcgactgg gaggggctga gcatgcagta cgtccacact tgttacaggg tcctggacct
      241 cgacaagagc atagatttct acgagaacaa gctcgggctg gaactcgctc gcaagtcacc
      301 cgtgggcgac gacgcgacga acgccttctt cgccgtgcca ggcgatcccg agccccggct
      361 ggagcttacc ctgaaccatg accaggacga gccctacgag ctcggtacgg ggtacagcca
      421 tgtcgcgttt gccgtggagg atctcgacgc cctcgcagag aggctcgagg aggccggggg
      481 cgtagagttc gagagcaagc cccacgccat gagcagcggg acacggatct ttttcgtcaa
      541 ggacccggac ggctaccgca tagagttcat cgaacgcgcg tagcgcacca cccggacgac
      601 tcgaaaagga accggccatc ctcgaagacg gccaccgtct tgccctgttc gaatgctgac
      661 ggctgatagc tgttgcccca accacagtcg tttatcgcac ggctctaaag catatccgtg
      721 gcggcgccga ctaataggtc gtggcatcgg cacgacgaaa agacttcggc gactgcgagg
      781 atgagggcgg gtataccctg cccgaggttc tgaccgcggt ggccatcatg agcgttattc
      841 tggccatcgg ggtgatcgtg ttgctggcct tgctggagcg gtggcgggtg gaggccgcgg
      901 ccgaccagtt cgccgccgac cttcggctcg tgcacacgag ggccaccgag cagctcaccg
      961 actggagggt ggtgttcatg gatgacggta cacccctggt cggctgctcc gccgcggact
     1021 actgcctggt caagctgagt ctgccctacg gggccggtga cccggcgccg gcgctcgacg
     1081 gttcggtaag gccggcggtg cgagagttgc cggaagggac caggataaag gaggtgacct
     1141 tcgatcccga ctgctcgcac ggggacagga acgctgccgt tccgccaagc acctgcgggg
     1201 caaccgctac acaagctggg gcgacccgga cgctcgagtt caaccccaac ggcaccgtca
     1261 ggacgctcag gccgggacag agcgggaccg tcgtgatcag ctccgacgac ggaagtccgg
     1321 tctgtccggt cgtcttcaac gctcccacgt ccaggatacg tatcggtgag atctactacc
     1381 cgtagaaccg cgttcgaccc tggctactcc agcggggagg tcatggttgc gatcgtggtc
     1441 ctgaccgtcg ccatagtccc catggccggc atgttcggtg cggcgataga ggccgccgat
     1501 gcgggcggag aatacgacag ggcgcggtcc tgcgcggtcc agaggctgga gcagataaag
     1561 agcctgccgt acgagtcggt cgaggccggt ttgcccgatg gtgtctgcga accgatcggc
     1621 cttcggtacg atgtggccac ctatccggtt ggcaccgacc tcgccaccgg cccgggggag
     1681 gcggggttga cgatggtgac cgtcacggtc gattcggacg gaatttccta cggcatctcc
     1741 ggggtcgtgt cgaggtggtg aggcggcatg ggcaagcggg gttcacgctc gtcgaagttc
     1801 tggtcgcagc ttcgatgatg ttcgccgtgc tcttcgcgct ctacgcggta ttcgatgtca
     1861 gcgtcagggc tttcggatac ggcggcgaca gggtcgaggc cgtcgccaac gccaggctcg
     1921 tgatggacag gatgacgcgt gagatcaggg tcgcctaccc ctacgatcca acggcggacc
     1981 cgcccaggag gcacctgttt ctgaacccgc tcgaccccac ggagccggcg gtgccgaccg
     2041 cgacgcgtct cgccttcggc aacgagacga ccggagaccg caagatcggg gcctccgagg
     2101 tgatcggcta ctactcgagc ggccaagatc tgaaacggtc gaagggtggc agcgtccaga
     2161 ccctactcga cacggtcgcc acgaacgggc tgcgcctgac cccgtgccgg accgcggaag
     2221 attgtccccc tgccgtggcg ttgactgacg aagccgcagt ccgcctcctg cgcatagagc
     2281 tgaccgtcga ggtgcaacgg cgcggggaag accccgcaag acaaaggctg gccaccgacg
     2341 tttacctgcg aaaccgggtg gtcccgcgat gattacacgt ctccggcgag aggaacgggg
     2401 gatggtgctt gggctgacca tcatcatcgt tctggttgtg ggggtgatgg gggccgggct
     2461 tctgaccttt gtttccgccg acctgcagtc catcgtggcc gtcaacaagg gagagcaggc
     2521 cctccagctg gccgaggcgg gcatcgaggt tgcgaagtca catcttgcca acgaccccgg
     2581 cccgaccgac tggtcctcgg gcgaactcca cgcgcaaggc gtagacgcca actcggtcac
     2641 cgtcaccatc gaccagaacg ccggatcttt cgaggtcgtc tccacgggcc agtacggcga
     2701 gacgaagcgc aaggtcgaag ccgtcttcga ggtccaggat ggtgatccca ggctcatcgg
     2761 atggcgggaa ctctacgagt agcgatcggg ctacaggttt caggtttcag gaattctacc
     2821 tgacgcaccc tcccgagttc gtctccgctc attgttttgt cagctagtgt tgacacgctc
     2881 cgtgctgtca actatagttg acgtatggac gagtcggttg ggacacgggt tggtgggcct
     2941 gaggatccgc gggctgggtt ggaggccgtt gtggcgctca ggcggacgct ggaggcgttg
     3001 gaggctgcgc aggtagagaa cgcgttcgtt gcgggctggt cgtgggcccg gatcgccgag
     3061 gttctggggg tgagccgtca ggccgtacat aagaagcacg cggcgaggat cagggcgagg
     3121 tatcccggga gaccgccggg ggggaaggag gggggcggat gatcttccac cgtttcacgg
     3181 gagaggcccg aggcagcgtg gaggccgcgc tcgaggaggc caggatgctt ggccacgact
     3241 ccctgggcga tgaggacctt ctgctcggca ttctacgtgc cggtgagggg gtcgcagccg
     3301 aggccctcga atcgtttggt gtcacgctcg aagacgcgag gggggagtcg gaggcgatgc
     3361 tctcgggggc gctcgcttcg atcgggatct cgctggagga gatcaggaag gaagcggggg
     3421 acgctttcga cctgcggcta cccgagaaac gccgcatacc cttcgcccct ggggcgaaga
     3481 aggttctgga gcgggcgctc gcgagggcgg tgcggctcgg ggacagatac ctgggaaccg
     3541 aacatatcct gctcgccatc ctggagaacg ccgaaggcac ggccgtcagg atgctggcgc
     3601 gactcggcgt cgcaccggag gcgctggagg agcgcctgtt cgagctgcgc ggccgggctg
     3661 ctggctcgcg tggggtgtta gattctcccg tctctgtaaa cagaccccgg tctttcgccg
     3721 tccggaacgc tagaatgcgg ttatggagga gagagaagaa cgcggtgggg cagtcagggt
     3781 cgggatggtc tggggcggca tcggcggggc ggtggggctg ctagtctcgc tgctcggttc
     3841 gctgatcgga atcgtcgtcg ccgtctttat cgggatttcg tgcgggaagc gggccgccgc
     3901 tgccgggccg tgggccgggg ccgtctccgg gctcgtggcc ggggccatcg tcgcgcccgt
     3961 gtacgcgctc gggtccacca taggggcgct cttcgctgcg caggagatag gggctgcgag
     4021 gttggcgcag acgctctccg agttgctcgg gtcgaacgtt tcggccgacg aggcgtgggt
     4081 cttcatgctc ataagcctcg tcctctcggc ggtgatcgag atcattcttc tgacgggggt
     4141 cgcagcggcc gcgggagcct gggcaaagag gggctagcgg gcgcgctatc gtccggcggt
     4201 cggcctattc cgttctggcc gcctccgccg ccgtgtcgcc gaagacgagc ccaccctcgt
     4261 cggggcgctc tacgccggcc tcgtcgagcc ggcggtcctt ctggtatttg cggatcgcgg
     4321 ccttcagcgt gccgaggccg agggtggcgc acctgggccg cgaggcaacg acctggcggc
     4381 cgagttgttc gatcatctcg ttgtagtcca tgtcgagaac ctgctccatg gtcaggttgc
     4441 ccctgtggac ctcttccagg atcatgctgc tcgccgccat gctgatcgtg cagccctcgc
     4501 cctcgtaggt ggcgtccccg atgtgctcct ggtcttcggc gcccttgaaa tacaccgtga
     4561 ccacatcccc gcaaccggga ttaccccccg gcatggtcac gtctgcgtcc tccagcgcac
     4621 cccgatgccg cggcttctcg tagtgatcga gcagcacctg tatctgcaac tgcctgtcca
     4681 acaatcaacc ttccactgtg gtgtcgaata cctaccgacg atgattctat cgcaagtggg
     4741 tcggggggct cggtaatctt gctacagttt gatacactcg ccccatgaga gtcggaatag
     4801 tcggtctgcc aaacgtcggc aagtcaacgg tcttcaacag cctcacccgc gcgggcgccg
     4861 aggcgcagaa ctatcccttc acgaccatcg accccaacct gggcgtcgcc gccgtgccgg
     4921 ataggcgact ggacgcgctg gcgaagacga tgaagagcaa gcgcgtggtt cccgccacgg
     4981 tcgattttgt ggatatcgcc gggctggtgc gcggcgcgag ctccggtgag gggcttggca
     5041 accagtttct cgggcacatc agggagtgcg acgcgatagc gcacgtggtc cgttgcttcg
     5101 aggacgagaa cgtggtgcac gtgcacggcg gcgtcgaccc cgagggcgac atcgagacca
     5161 tcgacaccga gctactgctc gccgacctgg ccaccgtgga gcggcggctc gagcggatcg
     5221 ggcgggcggc gaagggcggt gacgccgcgc tccgggccga ggctgacgag ctggagcggc
     5281 tgagggatca tctctccgac ggtagcccgg cgcgggccta cccgcactcg gagacgctcg
     5341 cggaggccat ggacgccctc atcaccgcca agcccacgct gtacgtggca aacgtggacg
     5401 aggagtcggt ggctgaggga aacacctaca gctcgcgggt cgagaagctt gcggcgcgcg
     5461 agggcgccga ggcggtccgc atctccgcga ggctcgaggc cgaggtggtt gacctcccgg
     5521 aggaagaggc caccgagtac ttgcagatgc tcggggtcga gaccacgggt ttcgaggagt
     5581 tcgtacggtc cgcgtacagt ttgctggggc tcgtcacctt cttcaccgcg ggggagaaag
     5641 agagcagggc ctggaccgtc agccagggtt ccacggtacg tcaggccgcg ggccgcatcc
     5701 acacggacat ggagcgcggc ttcatcgccg ccgaggtcgg gggctggcaa gatatcgtca
     5761 acgccgcctc ctgggcaaag gccagggaag aggcgaaggt acgcctcgaa ggccgcgact
     5821 acctcataaa agacggcgac accgtgctca tccgcttcaa cgtctgagcc acatgccacc
     5881 cttcgtcagg cccggaaggc ccctcgcccg gtcccgcctc gcgctcgtca cgacgggtgg
     5941 cgtgcacctc cccgaccagc ggcgcttcga catagacgac ttcgaaggcg actgctcgta
     6001 ccgtgaggtc ccagccgacg ccgaagagct cacgtggacc cacgcttact tccgtcccga
     6061 cgaaggctcc gacctcgacg ccgtctttcc actagagacg ctgcggcagc tggccgaaga
     6121 aggcctcgtg ggcgggctcg gaccacgcca cttttccttc atgggcgcca tacacgaccc
     6181 gacccccctc gaggaagaga cggcccccga ggtcgcccga aagctgatcg aggacggcgt
     6241 cgacgcggtg ttgctaacgc cctcctgacc cctgtgccac cggtccgtcg ggctggtcag
     6301 caacgcgatc gagcag
//
LOCUS       DBNGVD010000052         2901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4380373, whole genome shotgun sequence.
ACCESSION   DBNGVD010000052 DBNGVD010000000
VERSION     DBNGVD010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4380373"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="AC16CM_01405"
     CDS             complement(<1..575)
                     /locus_tag="AC16CM_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3138512.1"
                     /translation="MLILIVEDERAIARFLERGLNAHGHRVLCAYDGEEGIRLAMEEP
                     VDFVLLDISLPRLDGHEVLRRIRLKHPDLPILMLTARDDTRNKVGALNAGADDYLTKP
                     FVFEELLARIRALTRRSGQPASAEIQADDLRIDLLSRHAWRKDRRIDLSSREFALLEY
                     FMRHNGQVLSRQQILSAIWDYDFEPASNVVDV"
     gene            712..>2901
                     /locus_tag="AC16CM_01410"
     CDS             712..>2901
                     /locus_tag="AC16CM_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3138513.1"
                     /translation="MAYGALKALRTDGHQQVGDEEHFRPPADYAGLLVYRYGLLPKPG
                     FEYVSQSSTAITGYSPEDYYADPDLDFEQVHPEDRLLLGDSRRFTRGPLVLRRYRKDG
                     ALIWTEQHEQPVYDGAGNLVAIEGVAWDVTERELAEGALRQSEGFRAVFGSATLGMAF
                     WSLDGKWLQVNPAMCEITGYTEEELLATDFQSITHPDDLEEDVEGLRRMFTGEVQYLY
                     SEKRYIHKSGREVWVFLSTSLVRDAEDRPHCVFGQIQDISEARKDKENLERMIRQNRS
                     ILASAGEGIYGLDLDERTSFVNPAAERMLGYEPGELAGRHQHDIVSHINAEGVPYPED
                     ECPMHASLRDGGVHSSSDEVFTRKDGSTFPVEYVSNPIWEGGEVVGAVVTFRDITERK
                     AAEEALRESEARFRLLAEKMSDLVCLHEPDGRYVYISPSCRRLLGYDPEELLGTDPYL
                     LFHPDDLSRIRAEAHDKALEGQAAVSVIYRIRKKSGEYTWLETVTEPIRNEDGEIVRL
                     QTSSRDVSERKRVERVLADAARAKADFLAEVSHELRTPLTVIRGNAEVGLEFDGDCEH
                     VELLREIVRETSTMSGMVEDLLFLARSESAAPPFRMDPVDANVLMRGLARRATSLAAE
                     HGATLDTTLEETGLVEVDPARVEQAVLALVDNAIKYGPAGQRITLRSCSREGELHIEV
                     GDRGPGIPEAELPRVFERFYRLESGGEPGNGLGLSIAQTVAEAHGGRI"
BASE COUNT          529 a          924 c          979 g          469 t
ORIGIN      
        1 acgtcgacca cgttggaggc cggttcgaag tcgtaatccc agatggcgga gaggatctgt
       61 tgcctggaga gcacctggcc gttgtggcgc ataaagtact cgagcagagc gaactccctg
      121 ctggagagat ctatcctcct gtccttgcgc caggcgtggc gcgagaggag gtctatcctc
      181 aggtcgtcgg cctgtatctc agccgaggcg ggctggccgg agcggcgggt cagcgcgcgt
      241 atccgcgcca gcaactcctc gaacacgaat ggcttggtca ggtaatcgtc ggcccctgcg
      301 ttcagggcgc cgaccttgtt gcgggtgtcg tcgcgggccg tgagcatcag gatgggaagg
      361 tcggggtgct tgaggcggat gcgccgcagc acctcgtggc cgtcgagccg gggcagcgag
      421 atgtcgagga gaacgaagtc aaccggctcc tccatggcca gcctgattcc ctcttcccca
      481 tcataggcac agaggaccct gtgcccatgc gcattgaggc ctcgctccag gaaacgcgcg
      541 atcgcgcgtt cgtcttccac gatcaagatc agcatcacta gtcctcgctt cccgatcaca
      601 agtataatcg atcatgatgc gaccgatcca ggtgaatttt cgagggccgt gaatattcag
      661 atcttactcc gccaaatccg ccgcgtcgcc ctgggggggg cgctggccgg gatggcatac
      721 ggggccctga aggcgctgcg aacagacggg caccagcaag ttggggacga ggaacatttt
      781 cgaccccccg cggactacgc cggcctcctc gtctaccggt acgggctgct gccgaagcca
      841 ggcttcgagt acgttagcca gtcttccacc gccatcacgg gctactcacc cgaggactac
      901 tacgccgatc ccgacctcga tttcgagcag gttcatcccg aagacaggct cctgctcggg
      961 gactcccggc gcttcacacg cgggccgctc gtgttgcgcc ggtaccgcaa ggacggggcc
     1021 ctgatctgga ccgagcagca cgagcagccc gtctacgacg gggccggcaa cctcgtggcc
     1081 atcgaaggcg tggcgtggga cgtcacggag cgcgagctgg ccgagggggc gctcaggcag
     1141 agcgaggggt ttcgggccgt gttcggctcg gcgacgctgg gcatggcgtt ctggtcgttg
     1201 gacggaaagt ggttgcaggt caaccccgcg atgtgcgaga tcaccggata cacggaggag
     1261 gagttgctcg ccacggactt ccagagcatc acccatcccg acgacctcga agaggatgta
     1321 gagggcctcc ggcgcatgtt taccggggag gtccagtacc tctactccga gaagcgctat
     1381 atacacaaga gtggccggga ggtctgggtc tttctgagca cgtccctggt gcgcgacgcg
     1441 gaggacaggc cgcactgcgt ctttggccag atacaggaca tatcggaggc gcgaaaggac
     1501 aaggagaacc tggagcgcat gatccgccag aaccgctcca tactcgcctc cgcgggcgag
     1561 ggcatctacg gcctggacct ggacgagaga acctcgttcg taaaccccgc ggccgagcgc
     1621 atgctgggct acgagccagg cgagctcgcc gggcgacacc agcacgacat cgtgagccac
     1681 atcaacgccg aaggcgtgcc ctacccggaa gacgagtgcc ccatgcatgc ctccctcagg
     1741 gacggcggcg ttcattcctc gagcgacgag gtcttcacgc gaaaggacgg ctccaccttc
     1801 cccgtggagt acgtgagcaa ccccatctgg gagggcgggg aggtcgtcgg cgcggtcgtc
     1861 accttcaggg acatcacgga gcgcaaggcg gccgaggagg cgttgcgcga aagtgaggcc
     1921 cgtttccggc tgctcgccga gaagatgagc gacctcgtgt gcctgcacga gccagacggg
     1981 cgctacgtct acatcagccc ctcctgccgg cgcctgctcg gctacgatcc cgaggagctt
     2041 ctgggcaccg acccctacct tctcttccac ccggatgatc tgtcacgcat ccgcgccgag
     2101 gcccacgaca aggcgctcga agggcaggca gccgtctccg tgatctaccg gatccgcaag
     2161 aagtcggggg agtacacctg gttggagacc gtcaccgagc ccatacgcaa cgaggacggc
     2221 gagatcgtcc gtctccagac gtcttcccgc gacgtcagcg aaaggaagcg cgtggagcgc
     2281 gtgctagccg acgccgcccg cgccaaggcc gactttctcg ccgaggtctc ccacgagctc
     2341 aggacgcccc tgaccgtgat caggggcaac gcggaggtcg ggctagagtt cgacggcgac
     2401 tgcgagcacg tcgagctgct gcgggagata gtcagggaga cgtccacgat gtcggggatg
     2461 gtggaggact tgctgttcct ggcgcgttcg gagtcggcag cgccgccgtt ccgtatggat
     2521 cccgtggacg cgaacgtctt gatgaggggc ctcgcgcgca gggcaacgtc gctcgccgcc
     2581 gagcacggcg cgaccctgga taccacgctg gaggagaccg gcctcgtcga ggtcgacccc
     2641 gcgcgggtcg agcaggctgt tctcgccctc gtggacaacg cgatcaagta cgggccggca
     2701 ggccagcgga taaccttgcg gtcctgctcc cgcgagggcg agctgcacat cgaggtcggg
     2761 gaccgggggc ctggcatacc ggaggctgag ctgccacggg tcttcgagcg cttctaccga
     2821 ctggaaagtg gcggggagcc gggcaacggc cttggcctct ccatcgccca gacggtagcc
     2881 gaagcccacg gcgggcgcat c
//
LOCUS       DBNGVD010000053         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2741202, whole genome shotgun sequence.
ACCESSION   DBNGVD010000053 DBNGVD010000000
VERSION     DBNGVD010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2741202"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC16CM_01415"
     CDS             <1..489
                     /locus_tag="AC16CM_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138514.1"
                     /translation="RTDYVDLLLMHWPPDGVPLGETLGAMRKLQDRGIVLHIGVSNFS
                     PALLEEATGHAEIFCNQVQYHPFRSQTELLDQARELDYLLTAYTPLSRGGVKDDATLQ
                     EIGEDHGKTATQVALRWLVQQDRVSAIPKATGGEHLAENLDVFDFELSEEEMDRVFSL
                     GR"
     gene            499..1605
                     /locus_tag="AC16CM_01420"
     CDS             499..1605
                     /locus_tag="AC16CM_01420"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR3138515.1"
                     /translation="MRARELGIRIGELAPGPEDAITDVSGVRVGHSTIVRGEGRLVVG
                     EGPVRTGVTVVCPREGLARDEPVFAGTSTLNGNGEMTGLEWIREAGTLSSPVAITNTH
                     SVGVVHDALVKAEMEASAGEEDHWTLPVAAETYDGTLNDINGQHITPDHVFEALASAS
                     AGPVEEGSVGGGTGMICHEFKGGIGTASRRVAEGGWTVGALVQANYGKRRMLRVDGAP
                     VGRILTTERVPSPYEQEETLRAGTGSIIVVLATDAPLLPIQCDRLAKRAAIGLARMGG
                     GLDEGSGDIFLAFATGNRGLPRDGLPEPPATVPVQMVTNDYMTPLFYAAAEATEAAIL
                     NALLAAGDMTGRDGITAYGLTPEMLLGALEEARR"
     gene            1635..2864
                     /locus_tag="AC16CM_01425"
     CDS             1635..2864
                     /locus_tag="AC16CM_01425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HIR3138516.1"
                     /translation="MVDGGRLWRRLSELAEIGRSESGGVTRLSFTDEERSAKDLVASY
                     MEEAGLSVREDAVGNLFGRREGRDPEAPAVLVGSHVDSVYNGGDFDGPLGVLAGIEVV
                     QAMEENGVLTERPIEVVAFTDEEGARFSFGMIGSRALAGHLFSGDLEHTDGDGLSVAL
                     AMREYGLDPDQVDDARRGQGSLAAYLELHIEQGRVLEAEDLPAGIVTGIAGPVWLRFS
                     LRGESGHAGATPMDRRHDALAAAAEIVGAVEEEASSTPSAVGTVGQIEARPGGINIIP
                     GGVDFSVDLRDIDVAGRDGVEGRIRERAEEVCARRGVELGIEELQRLDPAPCSEEVRS
                     VISGACEELGIRPHSLPSGAGHDGMHLASLCPMGMIFVRSKDGISHNPEEWSSKEDCE
                     AGCNVLYRSVLGLAGRG"
BASE COUNT          506 a          928 c         1039 g          411 t
ORIGIN      
        1 cgcacggact acgtggatct cctcctgatg cactggcccc ccgacggcgt gcccctcggc
       61 gagacgctcg gggccatgcg gaagctgcag gacagaggca tcgtgcttca tataggggtg
      121 agcaacttct cccctgccct cctcgaagag gcgaccgggc acgccgagat cttctgcaac
      181 caggtccagt accacccatt caggagccag accgagctgc tcgaccaggc acgggagctt
      241 gactacctgc tcaccgccta caccccactc tcccgtggcg gcgtcaagga cgatgccacg
      301 ctccaggaga tcggcgagga ccacggcaag accgcgaccc aggtggccct tcgctggctc
      361 gtgcagcagg acagggtctc tgccatcccc aaggccacgg gcggggagca cctcgccgag
      421 aacctagacg tcttcgattt cgagctgtcg gaagaggaga tggaccgcgt cttctctctg
      481 ggccggtagg cgggccgcgt gcgcgcccgc gagctgggga taaggatcgg ggagctcgcc
      541 ccggggccgg aagatgcgat caccgacgtg agcggcgtga gggtcgggca ctccaccatc
      601 gtgcgcggcg aaggtcggct cgtcgtcggc gaagggccgg taaggaccgg tgtcacggtc
      661 gtatgcccgc gcgaggggct tgcgcgcgac gagcccgttt ttgccgggac cagcaccctc
      721 aacggcaacg gggagatgac ggggctggag tggatccggg aggccggaac cctctcttcg
      781 cccgtcgcca taaccaacac ccacagcgtc ggcgtggtgc acgacgccct ggtaaaggca
      841 gagatggagg caagcgccgg cgaagaggac cactggaccc tccccgtcgc cgccgagacc
      901 tacgacggca ccctgaacga catcaacggc cagcacatca ccccggacca cgtattcgaa
      961 gccctcgcct cggcgagcgc aggtccggta gaagaaggct ccgtcggcgg gggtaccggc
     1021 atgatctgcc acgagttcaa gggcggtatc ggtacggcct cgcgccgggt cgccgagggc
     1081 ggctggacgg tcggcgccct ggtgcaggcg aactacggaa aacgccggat gctacgcgtc
     1141 gacggggcgc cggtgggacg gatcctcacc acggagcgtg ttccctcgcc ctacgagcag
     1201 gaggagacgc tgcgggcggg gacgggctcc ataatcgtgg tcctcgccac agacgccccg
     1261 ctgctgccca tccagtgcga caggctcgcg aagcgcgcgg ccataggatt ggccagaatg
     1321 ggaggtggcc tcgacgaagg gagcggggac atctttctcg ccttcgccac tggcaaccgc
     1381 ggccttccgc gcgatgggct ccccgaaccg ccggcgaccg tgcccgtaca gatggttacc
     1441 aacgattaca tgacgccgct cttctacgcg gcggccgagg ccaccgaggc ggccatactg
     1501 aacgccttgc tcgcggccgg cgatatgacg ggacgcgacg gcataacggc ctacggcctc
     1561 accccagaga tgctcctcgg cgcgctcgaa gaggcgcggc gttgaagccc gaaaaaccgg
     1621 gaggtaacgc agggatggta gacggcggca ggttgtggcg caggctctcc gagcttgcgg
     1681 agataggccg gagtgagtcg gggggcgtga cccgcctctc gttcaccgac gaagaacggt
     1741 ccgccaagga cctcgtcgcc tcctacatgg aggaggccgg cctctcggtc cgcgaggatg
     1801 ccgtcgggaa cctgttcggc cgcagggagg ggagggaccc cgaagccccg gccgtcctcg
     1861 tcggctccca cgtcgactcc gtgtacaacg gtggtgactt cgacgggccg ctcggggtgc
     1921 tcgccggcat cgaggtcgtg caggcgatgg aggaaaacgg cgtgcttacc gaacgcccga
     1981 tcgaggtcgt cgccttcacg gacgaggagg gcgcccggtt ctcttttggc atgatcggca
     2041 gccgcgccct ggccggacac ctcttctccg gggatctgga acacacggac ggggacggtc
     2101 tctccgtggc cctggcgatg cgggagtacg gcctggatcc agatcaggta gatgacgcca
     2161 ggcgcgggca gggttccctg gcggcctacc tggagctgca catagagcag ggcagggtac
     2221 tcgaggccga ggacctgccg gccggcatcg taacgggcat cgctgggccc gtgtggctaa
     2281 ggttctcgct gcggggcgag tcgggccacg ccggggccac accgatggac aggcgccacg
     2341 acgcgctggc cgccgcggcg gagatagtgg gtgccgtgga ggaggaggcc tcctccaccc
     2401 cgtcggccgt ggggacggtc ggccagatcg aggcgaggcc cggcggcatc aacatcatcc
     2461 ctggcggggt ggacttctcg gtcgacctgc gcgacataga cgtagccggg cgcgacgggg
     2521 tggaggggcg cataagggaa cgggccgagg aagtctgcgc ccggcggggt gtggagctcg
     2581 gtatcgagga gctgcagcgg ctcgacccgg cgccgtgctc tgaagaggtc cgctccgtca
     2641 tctccggggc gtgcgaggag ttgggtataa ggccccactc gttgccaagc ggggccgggc
     2701 acgacgggat gcacctggca agcctgtgcc ccatgggcat gatcttcgtc cgctcgaaag
     2761 acggcataag ccacaacccc gaggagtgga gcagcaagga agactgcgaa gccggatgta
     2821 acgtcctgta tcgtagcgta ctcggcctgg ccggccgagg ataatcccag ggaggggggc
     2881 agag
//
LOCUS       DBNGVD010000054         6403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4016903, whole genome shotgun sequence.
ACCESSION   DBNGVD010000054 DBNGVD010000000
VERSION     DBNGVD010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4016903"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="AC16CM_01430"
     CDS             complement(<1..1132)
                     /locus_tag="AC16CM_01430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR3138517.1"
                     /translation="MAEQYDLVIIGGGNAGYIPAIRASQLGMSVALIEKREGGHLGGT
                     CLNVGCIPTKALLQTAHLLHDAKNGEEYGVMIGDLEFDYPQAAKRREQVVTQLRKGVQ
                     GLMKKNKVTVYYGVGSFVEPKKIKVETDDGEEELEAENVLISTGSAVNSLPGLEFDGE
                     KVISSDDIVTNNESYPESVIILGSGAVGVEFASMYNDFGTEVTIVELLDRLVPLEDPE
                     ISAALQKEFENRGITVLTSTQADPESLEKTDDGVKIKIASAGASEQEEAEGEGGGYGG
                     EDPPTGEAEDGETLEAEALLVAVGRKTVTEDLNLDVTNVEVGDRGIIQVDGYGRTAED
                     GVYAAGDVVGGFWLAHEAGHEGIIAVEHMAGENPMPKDRNLVP"
     gene            complement(1261..2121)
                     /gene="mutM"
                     /locus_tag="AC16CM_01435"
     CDS             complement(1261..2121)
                     /gene="mutM"
                     /locus_tag="AC16CM_01435"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR3138518.1"
                     /translation="MPELPEVETIKEDLRGLVVGSRIERVEVLDTSLVEQPSTEEFTG
                     RLEGAGITDARRRAKHLIVELDSGDSLVFQLKIGGQLLLVPPVEEPETALMLVLYLDG
                     ERRLFLRDETSFTRARLLGAGELEERLSSLGPEPLEGGFTVEYLPREIGARRAQIKPL
                     LLDQKVVSGIGNIYVDEILYDAKLHPRRKANTLSEGEWRALYEAIRSNLAAGVEHRGT
                     TVRLYRDVLDRPGEHQNYLRVFEKQGQPCPRCKGEVEREKVSGRPSHFCPDCQREDGS
                     GNGGGVESLW"
     gene            complement(2152..2886)
                     /locus_tag="AC16CM_01440"
     CDS             complement(2152..2886)
                     /locus_tag="AC16CM_01440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3138519.1"
                     /translation="MGGWYGEDLAYIHDVGHADFALRSAPGIREILERNGIRGGLIVD
                     LGCGTGLLARELVEAGYDVFGIDISGAMIEIARQRTPEADFRVASLFEVGIPCCGAVT
                     AISEVLNYLFDPENEKWGLGRVFRRVYDSLAPGGVFVFDVLGPGQVPPGTRVKGFSVG
                     QDWAVLSEREEDARRSTMERRIVGFRKVGDYYRRIDEVHRVRLYEPQELVAELERVGF
                     RVRTMRAYGDLPLAEGHTAFVARKPA"
     gene            complement(2890..3240)
                     /locus_tag="AC16CM_01445"
     CDS             complement(2890..3240)
                     /locus_tag="AC16CM_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138520.1"
                     /translation="MPELPEVETIKEDLRGLVVGSEIGPEGPQRAAYREENRVNAEEF
                     LGRLKREVIGHPSLSHPFLERFAEDEGGQRRVRESAMRSLDARSVMLDGLYRVVYKEP
                     VPTIEVPQVRLTGG"
     gene            3640..5706
                     /locus_tag="AC16CM_01450"
     CDS             3640..5706
                     /locus_tag="AC16CM_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3138521.1"
                     /translation="MARSIEQHDGEHGAASASHAGHGGGKSAGGHDKHAGHSVAMFRD
                     RFWASLVMSVPVVLYSEMIQMWLGFTMPEFPGSMWISPVLGTFVFLWGGQPFLVGGFR
                     EARDRQPGMMLLISMAITVAFVASAATTLGFFDLDFWWELALLVTIMLLGHWLEMRAI
                     GQASGALEALAALLPDDAERVTADGTEKVSLDVLEAGDVVLVRSGGRVPADGEVVDGA
                     AELDEAMITGESKPVPKGVGDRVVAGTVATDSAIRVKVTAVGEETALAGIRRLVEEAQ
                     ASRSRAQVLADRAAALLFYFATAAAVITFVVWMALGNPTRAIENTVTVLVISCPHALG
                     LAIPLVIALSTALSARNGILVKDRLALERMRNVDTVLFDKTGTLTKGEPAVTGVAAAN
                     GDGDALLALAASVEADSEHPLARAIVAAAREKDLSVPTASGFRSITGRGVEAKVGEVK
                     VSVGGPSLLRETGVEEPDELRGVVGDWKRRGASVLHVLREDEVAGALALEDEVRSESR
                     EAVKELHEAGVRVVMITGDARQVAEAVAADLGIDEVFAEVLPEDKDKAVAELQGRGEK
                     VAMVGDGVNDAPALARSDVGIAIGAGTDVAIESAGVVLASNDPRAVLSVVRLSKAGYR
                     KMVQNLLWAAGYNVITVPLAAGVLAPIGIILAPAIGAILMSVSTIVVALNAQLLRRLD
                     LRPEQA"
     gene            5781..6233
                     /locus_tag="AC16CM_01455"
     CDS             5781..6233
                     /locus_tag="AC16CM_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138522.1"
                     /translation="MGQAAPGIPGHRTPIALVLATALLLCHGVFGASHVLAHGDPARA
                     SGPAVNERPHGHTAAVVAPDPHHVAGAVAGVPAGVEGVDGAHEGTPAEYFAVLQALVG
                     VVLLAAYFSAARAPLPARARPVGIRIGEPSPPPLPRGTSPPLLQVFRL"
BASE COUNT          997 a         2172 c         2066 g         1168 t
ORIGIN      
        1 gcgggaccag gttgcggtcc ttgggcatcg ggttctcgcc ggccatgtgc tcgacggcta
       61 tgatgccctc gtgcccggcc tcgtgggcga gccagaagcc gccgaccacg tccccggccg
      121 cgtacacacc gtcctcggcg gtcctgccgt acccgtcgac ctggatgatg ccgcggtcgc
      181 cgacctccac gttggtaacg tccaggttca gatcctcggt cacggtcttg cgtccgacgg
      241 ctacgaggag cgcctccgcc tcaagggtct cgccgtcctc ggcctcaccg gtgggcgggt
      301 cctcgccgcc gtagccaccg ccctcgcctt cggcctcctc ctgctcgctg gcgcccgcgc
      361 tcgcgatctt gatcttgacg ccgtcgtcgg tcttctccag gctctcgggg tcggcctggg
      421 tgctggttag caccgtgatg ccgcggttct cgaactcttt ctgaagcgcg gcggagatct
      481 ccggatcctc gagcggcacg aggcggtcga ggagttccac gatggtcacc tcggtgccga
      541 agtcgttgta catgctggcg aactccacac ccaccgcgcc ggagccaagg attatcaccg
      601 actcggggta gctctcgttg ttggtaacga tgtcgtccga ggagatgacc ttctcaccgt
      661 cgaactccag gccgggcagg gagttaacgg ccgatcccgt agagatcagg acgttctccg
      721 cctcaagctc ctcctcgccg tcgtcggttt cgaccttgat cttctttggc tcgacgaagc
      781 tgcccacgcc gtagtagacg gttaccttgt tcttcttcat caggccctgc acgcccttgc
      841 gaagctgggt gactacctgc tcgcggcgct tggcggcctg cgggtagtcg aactcgaggt
      901 ccccgatcat gaccccgtac tcctcgccat tcttggcgtc gtgcagcagg tgggccgtct
      961 ggagtaacgc cttggtcggg atacatccca cgttcaggca ggttcccccg aggtgcccgc
     1021 cctcacgctt ctcgatcagg gccacgctca tccccagctg gctggccctt atggcaggta
     1081 tgtacccggc gttcccgccg ccgatgatta ccagatcgta ttgctcagcc actctcccgc
     1141 tcccttcggt cgcttaggtt tcaggctaca gggttcaggt tggtcatcag ttgtcccgcg
     1201 cctctggtcg tacgaccggt atttcctgat gcctgttgcc tgaaacctgt agcctcctct
     1261 tcaccacagt gattctaccc ctcccccatt ccccgagcca tcttccctct ggcagtccgg
     1321 acagaagtgt gagggcctcc cgctgacctt ttccctctct acctccccct tacaccttgg
     1381 acagggctgc ccttgcttct cgaagacccg caggtagttc tggtgctcgc cgggcctgtc
     1441 caacacgtcc ctgtacaggc gcaccgtggt gcccctgtgc tccacgccgg ccgccaggtt
     1501 cgaccttatc gcctcgtaga gtgccctcca ttccccctcc gagagcgtat tggccttgcg
     1561 cctcgggtgc agcttcgcat cgtacaggat ctcgtcgacg tagatgttac ctattcccga
     1621 aacgactttc tggtcgagga gcaggggttt gatctgggcc ctccgggcgc cgatctcacg
     1681 cggcagatac tcgaccgtaa acccaccttc gagcggctcc ggcccgagcg aagagagcct
     1741 ctcctcgagc tccccggcgc cgagcagccg cgcccgcgtg aagctggtct cgtcccgcag
     1801 aaagagcctt ctctctccgt cgaggtacaa cacgagcatt agcgccgtct cgggttcttc
     1861 gaccggcggc acgagcagca gttgaccccc gatcttgagc tgaaagacga gcgagtcccc
     1921 gctatctagc tcgactatga ggtgcttggc cctgcgccgc gcgtccgtga tcccggcgcc
     1981 ttcgagccgt ccggtaaact cctccgtgga aggctgctcc acgagggagg tatccaggac
     2041 ctccacgcgc tctatcctgg atccgacgac gagcccgcgc aggtcctctt ttatggtctc
     2101 gacttccggc agctcgggca tgggctagtt atacccacct tccacgctat ctcacgccgg
     2161 tttccgggcg acaaaggccg tatggccctc tgcgagcggc agatccccgt aagcccgcat
     2221 cgtcctaacc cggaaaccca cccgttcgag ctccgcgacc agctcctgcg gttcgtacaa
     2281 tcgcacgcga tggacctcgt cgatccgcct gtagtaatcc cctacctttc ggaaaccgac
     2341 gatgcgccgc tccatcgtac tccgcctggc atcctcttcc cgctcggaga gcaccgccca
     2401 gtcctgcccc acgctgaaac ccttcaccct cgtaccgggc ggcacctgtc caggaccgag
     2461 cacgtcgaag acgaagacgc cgccaggagc cagggagtcg tagacgcgcc ggaacacccg
     2521 tcccaggccc cacttctcgt tctcgggatc gaagaggtag ttgagaacct cgcttatggc
     2581 cgtgacggcc ccgcaacacg gtatcccgac ctcgaacaac gacgccaccc gaaaatcggc
     2641 ctccggtgtc ctctggcgcg cgatctcgat catcgccccg gagatgtcta tgccgaaaac
     2701 gtcgtacccg gcttcgacca actcacgtgc caggagcccg gtcccgcagc cgaggtccac
     2761 gataaggccc ccgcgtatcc cattgcgctc cagtatctct cttattcctg gtgccgagcg
     2821 gagggcgaag tcggcgtgcc cgacgtcgtg aatgtaggcc aggtcctccc cgtaccatcc
     2881 gcccaaaccc tagcccccgg tgagccggac ctgagggacc tcgatggtcg ggacgggctc
     2941 cttgtagacg accctgtaga ggccgtcgag catcaccgag cgggcatcga gggagcgcat
     3001 ggcgctctcc cgaaccctac gctgcccccc ctcgtcctcc gcgaaccgtt ccaggaaagg
     3061 atgcgagagc gagggatgcc cgatcacctc ccgcttcaag cgccccagaa actcctcagc
     3121 gttcacccga ttctcctccc gatacgcagc acgctgcggc ccttcgggcc ctatctccga
     3181 gccgacgacg agcccgcgca agtcctcttt tatggtctct acctctggca gctcgggcat
     3241 gggctagtta tacccatctt cggagttttc ccacgccggt ctccgggcgg gaggtcgcgg
     3301 caagaacgca tggcccaaac ccggaagccg gcccgttcga ccgtcgcggg cagcccagct
     3361 gatcgcacaa ccgctcgcga tggacctcgt cgctccaccc gtggtggtca tcccccggaa
     3421 acggaccgtg cgaatagcgc gctggcaagg ccagtccccg gcgcattgct ggatccttgc
     3481 acgatcgccg gcatcccaga aggccaagac cctcgacttg acatacccta ggggggtata
     3541 gtacagtcag gattgaaaag tagctccgga tcccgagcgt acccggtctg acgaaggcgt
     3601 tcgagatctg ggcggggatt gtcgagaggg gcttggcaaa tggcgcggag cattgagcag
     3661 cacgatggag agcacggggc ggcgtcggcc tcgcacgcgg ggcacggcgg cgggaagtcg
     3721 gcaggcgggc acgacaagca cgctgggcat tcggtagcga tgtttcggga ccggttctgg
     3781 gcgtcgctag tcatgtcggt gccggtggtg ttgtacagcg agatgatcca gatgtggctc
     3841 ggcttcacga tgccggagtt tcccggttcg atgtggatct cgcccgtgct cggcacgttc
     3901 gtgttcttgt ggggcgggca gccgttcctg gttggcggtt tccgggaggc gcgggaccgg
     3961 cagccgggga tgatgctgct tataagcatg gcgataacgg tcgctttcgt ggcgagcgcc
     4021 gccaccacac ttggtttctt cgacctggac ttctggtggg agctggccct gctggtgacg
     4081 atcatgcttc tcggccactg gctggagatg cgggccatcg ggcaggcttc cggtgctctc
     4141 gaggcgctgg cggcgctgtt gccggacgac gcggagcgcg tgacggcaga cgggacggag
     4201 aaggtctccc tggacgttct ggaggctggc gacgtggtgc tggtgcgttc gggcgggagg
     4261 gtgccggccg acggcgaggt cgtcgatggc gcggcggagt tggacgaggc catgatcacg
     4321 ggcgagtcaa aaccggtccc caagggcgtc ggcgacaggg tggtggccgg aacggttgcc
     4381 accgactcgg cgatccgggt gaaggtgacg gccgttggcg aggagacggc gctcgccggg
     4441 atacgcaggc tcgtggaaga ggcgcaggct tcgcgttcgc gggcgcaggt tctggcggac
     4501 agggctgcgg cactgctgtt ctacttcgcc accgcggcgg cggtgatcac gttcgtcgtg
     4561 tggatggcgc tcggaaaccc gacgcgcgct atcgagaaca cggtgacggt gctcgtcatc
     4621 tcctgcccgc acgccctggg gctcgcgata ccgctcgtga tcgcgctctc caccgccctc
     4681 tccgcccgca acggcatcct ggtaaaggac cgcctggccc tggagcggat gcgaaacgtg
     4741 gacacggtac ttttcgacaa gacaggcacg ctcacaaagg gcgagcctgc cgtgaccggc
     4801 gtagccgccg cgaacgggga cggtgacgcg ctgctcgccc tcgcggcctc cgtcgaggcc
     4861 gactcagagc acccgctcgc ccgcgccatc gtcgctgcgg cgagggagaa ggatctctcg
     4921 gttccgaccg cgagcggctt ccgctccatc accgggcgcg gtgtggaggc caaggtgggc
     4981 gaagtgaagg tatctgtcgg cgggccgtcg ctgctcaggg aaaccggcgt cgaagagccg
     5041 gatgaactgc gcggggtggt tggggactgg aagcgacgcg gggcctctgt gctgcacgtg
     5101 ctacgcgagg atgaggtcgc aggtgcgctg gcgctcgaag acgaggttcg gtccgagtcc
     5161 cgcgaggcgg taaaggagct gcacgaagcg ggggtgcggg tcgtcatgat caccggcgac
     5221 gcccggcagg tcgccgaggc cgtggccgct gacctgggga tcgacgaggt cttcgccgag
     5281 gtgctgccgg aggacaagga caaagccgtg gccgaattgc agggacgcgg cgagaaggtg
     5341 gcgatggtcg gcgacggggt caacgacgcg cccgccctgg cgcggtccga tgtcggtatc
     5401 gccatcgggg ccgggacgga cgtggcgata gagtcggcgg gggtggtgct ggcatcgaac
     5461 gatccacgcg cggtcctcag cgtggtacgg ctctcgaagg ccggctacag gaagatggtc
     5521 cagaacctgc tgtgggcggc gggatacaac gtgatcactg tccccctcgc cgccggggtt
     5581 ctcgccccca taggcatcat cctggccccc gccatcggcg ccatcctgat gagcgtctcg
     5641 accatcgtcg tggccctcaa cgcacagcta ctccgccggc tggacctgcg gccggagcag
     5701 gcctaggtcc gatgctcgta gcctctggtc agaacgggaa aaacggtgtc cgccgcccct
     5761 cccatgcgag cttcggtggc gtggggcaag cggcgccggg gattcccggg caccgcaccc
     5821 cgatagcact cgtacttgct acggcattgc tgctctgcca cggcgtcttc ggggcctcac
     5881 acgtactcgc ccacggtgat ccggcgcgtg cgtcgggccc cgcggtgaac gaacgccccc
     5941 acgggcatac tgccgcagtc gtggcccccg acccgcacca tgttgcgggc gcggtggccg
     6001 gtgttccggc gggggtcgaa ggcgtagacg gcgcgcacga ggggacgccc gcggagtact
     6061 tcgcggtcct ccaggcactg gtcggcgtag tcctgcttgc cgcttacttc tccgccgccc
     6121 gggcgcctct accggccagg gcgcgaccgg tcggcatccg tatcggagag ccaagccccc
     6181 cgccgcttcc ccggggaacc tccccacccc tcctgcaggt gttccggctc tgagccatcc
     6241 cctcgcgcct cgcggggcgc gtagacacgc ggtctagata cggtcaaaca cctgaatcag
     6301 agaggcaaac tcgtgaagat gacgcatgga aggatcggcc tcgccgggct gctggtagcg
     6361 ctcgcgctcg tgctcgccgc gtgcggaggc gccggcgggt ccg
//
LOCUS       DBNGVD010000055         4411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1466390, whole genome shotgun sequence.
ACCESSION   DBNGVD010000055 DBNGVD010000000
VERSION     DBNGVD010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1466390"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC16CM_01460"
     CDS             <1..381
                     /locus_tag="AC16CM_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3138523.1"
                     /translation="TNVGYFDQDTRSVPPDDTALQVVAKTGCDETLARTVMGRMGVRR
                     ETVNKRASRLSSGERAKVILAGIILGDNNLLILDEPTNYLDIETQDVLLEALEDFPGG
                     MLFVSHDRHFVDRLATENLSLGLT"
     gene            386..1819
                     /gene="menE"
                     /locus_tag="AC16CM_01465"
     CDS             386..1819
                     /gene="menE"
                     /locus_tag="AC16CM_01465"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HIR3138524.1"
                     /translation="MSSEDLSAPCPLRMAALSTPEAPAVVGPQSTITYEELDGRVSAA
                     AFRLRELEPGSRVALYLPRDERYVILMLAVIRAGHVACPISDRLPPKGVAQLLEKAAC
                     EALISEDEELLRTAGTELHGSKPESLLEVDGQRAGPAEIPLERHATIIFTSGSTGTPK
                     ATLHTFGNHYYNAIGSNANIALRPGDRWLHSLPLYHVGGLSILFRCLLAGATIALPEH
                     GSPIGESIASLDATHVSLVSTQLQRLLRENNELGGLKAVLMGGGPVAASLVDKAVSRG
                     LPIHTSYGLTEMASQVTTTPPGASTGELRTSGRVLPHREVSVSERGEILVRGETLFAG
                     YVDGESLDLPSDAGCWFRTGDLGDLGGNGYLRVLGRKDNLFISGGENIQPEEIEDALC
                     RLDGVDEAVVVPVPDREFGRRPVAFVRTEADPARFEDLARALELVLPRFKIPVAFHGW
                     AEESGEMKVDRASFHRRALRLHPDRRD"
     gene            1981..2943
                     /locus_tag="AC16CM_01470"
     CDS             1981..2943
                     /locus_tag="AC16CM_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138525.1"
                     /translation="MTRAGDLRAWAVLVVGAACAAVVAVIAGVALDAGEARAATPTRA
                     TIEATQFTVQSGQTGEAFAVCPGTKRAVGGGVVQSGPAVVGISVAASGPLDGTGVTLN
                     TRDGDVAKQWYAAADNFTATQRIYKVFAICSSNSTATIEATPITIQPGQDLGEEFAVC
                     PGNRRVLGGGVVQSGSPDDIVTMASGPLDGTGFTSQTVTGDVAKQWYAAVEVISGEQR
                     TLKVFAICSGDSSARIKASAFSVESDQTGEAFARCGSSKRALGGGVVQSGTPDDLLTR
                     ASGPLDGTGVTLETRDGDVAKQWYAAQRNNSGPLRQFKVFAICE"
     gene            complement(3003..4364)
                     /locus_tag="AC16CM_01475"
     CDS             complement(3003..4364)
                     /locus_tag="AC16CM_01475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR3138526.1"
                     /translation="MDLHIRGFALPLDYTYRIKVPHRTGQLARVAGTISDGDGLIGDV
                     TTINVGREASVREITLEVDDKEQAERIVEKLRDLEGVEVLWARDRALLKHEGGKLVIE
                     SAHPVRTVQDMRDVYTPGVARACQAISEDPALARELTMIGRSVAICTNGTRVLGLGDI
                     GPVASMPVMEGKAVFYHQLAGVSAMPILIDTKDVNEFVETVVRISPTFGGIHLEDISA
                     PECFEIEARLKEALPQPVMHDDVHGTAVVTLAAVLVACRHAGLTLEDEVVGQIGLGAA
                     GYGIAALMVDAGAERVLGSDPNPLSHERAAAKGIEITDMETVMAEADLVVATTGVPGL
                     IEPGMVREGQVILALTNPYPEIEPNVALGAGAAFAADGKSVNNVLGYPGIFRGALLSG
                     ASQINAEMKLATAEVLASLTTGSELVPDALDPDVHLKVASAVREAAIESGVARLDRAP
                     TGL"
BASE COUNT          782 a         1460 c         1410 g          759 t
ORIGIN      
        1 accaacgtgg gctacttcga ccaggacacc aggagcgtgc cgcccgacga tacggcgctg
       61 caggtggtcg cgaagaccgg gtgcgacgag accctcgcaa ggaccgtgat gggcaggatg
      121 ggggtaagac gggagaccgt aaacaagcgg gcctccaggc taagctcggg cgagcgggcc
      181 aaggtgatcc tggccggcat catcctcggg gacaacaacc tcctcatcct ggacgagccg
      241 acgaactacc tggatataga gacccaggac gtgctgcttg aagcgctgga agattttccc
      301 ggcggcatgc tcttcgtctc ccacgaccgc cacttcgtgg acaggctcgc aacagagaac
      361 ctgtcgctcg ggctaacata gaggcgtgag cagcgaggat ctatccgcac cctgtcccct
      421 gcggatggcg gccctctcca ccccggaggc gcctgccgtc gtcggaccgc agagcaccat
      481 cacctacgag gaactggacg gccgggtctc ggccgccgcg tttcgcctcc gggagctgga
      541 accaggaagt agggtggcgc tctacctgcc cagagacgag cgttacgtca tcctgatgct
      601 ggccgtgatc cgggccgggc acgtggcctg tcccatcagc gaccgtctcc caccgaaagg
      661 cgtcgcgcag ctcctcgaga aggccgcctg cgaagctttg atctccgaag acgaagaact
      721 cctgcgaacg gccggcaccg aactacacgg atcgaagccc gaatccctac tcgaggtcga
      781 cgggcaacgt gcgggaccgg ctgaaattcc cctggagcgc catgcgacca taatcttcac
      841 ctccggcagc acgggtaccc cgaaggccac gctccacacc ttcggcaacc actactacaa
      901 tgcgataggt tcgaacgcca acatcgcgct ccggccgggt gacaggtggc tccactcgct
      961 gccgctctat cacgtcggtg gactctcgat cctgttccga tgcctgctcg ccggagcaac
     1021 gatcgcgctt cccgaacacg gaagcccgat cggcgagtcc atcgccagcc tcgacgccac
     1081 ccacgtctcg ctcgtctcca cccagctcca gaggctactc cgcgaaaaca acgaactcgg
     1141 cggattgaag gcggtcctga tgggtggagg accggttgcg gcgtcccttg ttgataaagc
     1201 cgtctctcgc gggctgccga tccacaccag ctacggcctc accgagatgg cctcgcaggt
     1261 cacgacgacg ccaccgggcg cctcgaccgg ggaactccgt acttcgggcc gcgtgctgcc
     1321 ccatcgtgag gtctcggttt ccgagagggg tgagatcctg gtccgcggcg aaaccctctt
     1381 cgcgggctat gtcgatggcg aaagtctaga ccttccttct gatgcaggct gctggttccg
     1441 caccggcgac ctcggagacc tgggcggtaa cggctacctc cgtgtcctgg gtcgcaagga
     1501 caacctcttc atctccggcg gggagaacat ccaacccgag gagatcgaag acgccctctg
     1561 ccgcctggac ggcgtcgacg aggccgtggt cgtgcccgta cccgaccgcg agttcggccg
     1621 caggccggtg gcgttcgtgc gcacggaggc cgatcctgcg agattcgagg atctggcgcg
     1681 tgcgctcgaa ctggttttgc ctcgcttcaa gataccggtt gcctttcacg gatgggccga
     1741 agagagcggg gagatgaagg tggaccgggc ttctttccac agacgcgccc tgcgcttgca
     1801 ccccgaccgg cgtgattaga gcaccagact cgcgcgcacc gaatccccta gaacgcttct
     1861 cgcgcaggct tgccgggccg cggttccgcg ttcaaaaggc ggaagcagtt ctcatgacgc
     1921 ttttgtaaca aaagtacgtt atgctgggcg caaagtgatt cgttgaggaa aggagcagtc
     1981 atgaccaggg caggcgacct cagggcgtgg gcggtgctag tggtgggcgc cgcgtgtgcc
     2041 gcagtagttg ctgttatcgc gggcgtggcg ctagacgccg gagaggcacg agccgccacc
     2101 cccacccggg ccaccatcga ggcgacccag tttacggtcc aaagcggcca aacgggtgag
     2161 gcattcgccg tttgccctgg caccaagcgt gccgttggcg gtggcgtagt tcagagcggt
     2221 cctgctgtcg tcggcatttc cgtggcagcg agcggcccgc tggacggcac gggggtgacc
     2281 ctgaacacca gagacggcga cgtcgccaaa cagtggtacg ccgctgcgga caacttcacc
     2341 gcaacgcaac gcatctacaa ggtgttcgcg atctgctcga gtaattccac agccactatc
     2401 gaggctactc cgatcacgat ccagcccggc caagatctcg gcgaagaatt cgccgtgtgt
     2461 ccgggtaaca gacgcgtcct cggtggcggt gtggtccaga gcggatcacc cgatgacatt
     2521 gttacgatgg cgagcggccc tctggacggc accggattca cctcccagac cgtcacgggc
     2581 gatgtcgcca agcaatggta tgccgcggtg gaagtcattt cgggagagca gcgcaccctc
     2641 aaggtgttcg cgatctgctc gggggattct tcggccagga tcaaagcctc tgccttctcg
     2701 gtcgagagtg accagaccgg cgaggcgttc gccagatgcg ggtccagcaa gcgcgccctt
     2761 ggcggtggcg tggtccagag cggaacgccc gatgaccttc ttacgagggc gagcggcccg
     2821 ctggacggca cgggggtgac gctagagacc agagacggcg acgtcgccaa gcagtggtac
     2881 gcggcgcaaa ggaacaatag cgggccgctg cggcagttca aggtgtttgc catctgcgag
     2941 tagggaggtc gtcctggcgg aaggctccgg tatcctgttc cggggcgctt cgcgaagcgc
     3001 ccctacaacc ccgtcggcgc ccgatcgagg cgggcgacac cgctctctat ggcggcctcg
     3061 cgcacggcgc tggccacctt caggtgcacg tccggatcga gggcgtcggg gactagctcc
     3121 gagcctgtgg tgagggaggc cagtacctcg gcggtggcta gcttcatctc ggcgttgatc
     3181 tggcttgctc ccgagagcag ggcgcccctg aagatgcccg ggtagcccag gacgttgttg
     3241 acgctctttc cgtcggcggc gaaggcggcc cccgccccca gggccacgtt cggctctatc
     3301 tccgggtatg ggttggtgag cgcgaggatc acctgcccct cgcgcaccat tcccggttcg
     3361 ataaggcccg gcacgccggt ggtggccacc acgagatccg cctccgccat gaccgtctcc
     3421 atgtcggtga tctcgatgcc tttggcggcc gcgcgctcgt ggctgagcgg gttcgggtcg
     3481 gagccgagca cccgctcggc ccccgcgtcc accatcaggg ccgcgatacc gtagccggct
     3541 gccccgagcc cgatctggcc aaccacctca tcctcgagcg tcaaccccgc gtggcggcag
     3601 gcaacgagca ccgcggcgag cgtgaccacg gccgtgccgt gcacgtcgtc gtgcatgacg
     3661 ggctggggga gagcctcctt caggcgggct tctatctcga agcactcagg cgccgagatg
     3721 tcctccaggt gtatgccgcc gaacgtaggc gagatgcgta ccaccgtctc gacgaactcg
     3781 ttcacgtctt tcgtgtcgat caggatgggc atcgcggaga cgccagcgag ctggtggtag
     3841 aagacggcct tgccctccat cacgggcatg gaggctacgg gcccgatatc tcccaggcca
     3901 aggaccctcg tcccgttcgt gcagatggcg acgctgcgcc cgatcatggt cagctcgcgg
     3961 gccagggctg gatcttcgga gattgcctgg caggcccgcg ccacgccggg ggtgtacacg
     4021 tcgcgcatgt cctgcaccgt tctgacggga tgggccgatt cgatcacgag cttcccaccc
     4081 tcgtgtttga ggagtgcccg gtcgcgcgcc cagagaacct ccacgccttc caggtcgcgc
     4141 agcttctcca ctatccgctc ggcctgctcc ttgtcatcca cctctagcgt gatctcacgc
     4201 accgaagcct cacgtcccac gtttatggtg gtaacgtccc cgatcaggcc gtcaccatct
     4261 gagatcgttc cggcgaccct ggccaactgc cccgtgcggt gcggcacctt gatgcgataa
     4321 gtgtaatcca gcggcaaagc aaaccccctg atgtgcagat ccatcccatg ctccagaacc
     4381 ctgtaccggg attctatagg agaccccgtc c
//
LOCUS       DBNGVD010000056        10788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_8803, whole genome shotgun sequence.
ACCESSION   DBNGVD010000056 DBNGVD010000000
VERSION     DBNGVD010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_8803"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="AC16CM_01480"
     CDS             complement(<1..237)
                     /locus_tag="AC16CM_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138527.1"
                     /translation="MNGPEKKTVTTERGSLLTRPVVLLTLIAFAVLFGFQLLISVVPL
                     YADEAGGGSSGAGLATAVFMLSTVLTQIQMPRILR"
     gene            complement(362..1006)
                     /gene="lexA"
                     /locus_tag="AC16CM_01485"
     CDS             complement(362..1006)
                     /gene="lexA"
                     /locus_tag="AC16CM_01485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3138528.1"
                     /translation="MVPENWKARMEMLRHLARLEAGGKGPPSVRELGEAVGFRSSQTA
                     YKHLKRLEEAGYVGRGDGKARGIRLTEKGWEAAGEMPLLGRIAAGRGLEAVTVEDEAY
                     SLAAELVGSRLGRRRYLLRVVGQSMIGAHIADGDLLIVEEDEDPPDGTVVAALIGNGE
                     EVTVKRLYREKNEVGDMLRLKPENGDHQDLLIPAQDVKVQGRVVYVVHPPRGRH"
     gene            1336..1761
                     /locus_tag="AC16CM_01490"
     CDS             1336..1761
                     /locus_tag="AC16CM_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138529.1"
                     /translation="METSVSEIHCYPLRPEGFEEPLFPTRESRLAVLDHLLKSSWLSR
                     ASEAESRGGHAAHAWREPVEVENSDGRMKVSWASGRRQCRRERRIVEVLGRWREVRAW
                     WDEGLATDRDIFRVLLSGGSVVDLARENGEGWFLVGTLD"
     gene            1758..5027
                     /locus_tag="AC16CM_01495"
     CDS             1758..5027
                     /locus_tag="AC16CM_01495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3138530.1"
                     /translation="MTGDNFVHLHVRSGFSFGFGVARPGELVEAAAGMGMPALALTDR
                     DGLYGIPRFLEVAGTAGVLPIVGVEVSTVEGHIVLLAESMEGYRSLCRLITQYRTSSE
                     DRRRPVCPLSAVGEHAEGVICLTGAVPFGLLPRLVLGGRGQDAMRVLRSLSGAFGGDR
                     LYVELTDDQTSAGRRNVAKMAAFAQDAGVPVVATGEVAYLSPADHRLHEVLVASTNLT
                     ALPGPEYRPTDRLHLRSAGEMRRLFAGYPQALAHAAGIAERCAGGVDLSSAVHMPQAI
                     LPPGKTAGKVLLELTIAGARRRYRRDSQGPDVEEIKARLRRELSCIDSLGFAPYFLLA
                     HEAREIAKSKGIPVSGRGSAANSLVSYCLGLSGLEPFSNRLLFERFMHEERKDPPDID
                     LDFCSVRRDEVRFEMIRRYVRFGVAEAATVQTMSLRGAVRVAARALGHSPAEINDLCR
                     HVPTRFRDRNRIYAGLSGWEEALAEPAMRGHPLQDTRRYALLLELSTGLAGRTKEGGT
                     HSGGMVFGTAERHLSELVPLEPSGTDGLLRCQYDKDDLERVGLPKLDLLGLRMHTALH
                     RAGVLVSRRLGRKIDPYDLPPEDRDTYALIRTGRNAGMFQLESPGQMHLSRRLGPWRF
                     TDLVAQISLFRPGPVRGDLVTPYVMRKNGREPYQAILPELEDVLQPTYGVLVYQEQVL
                     TVANAVAGFTLVEGDLLRRAMTKDRGPGAMKELRQKFVRRAVENGVPAAEAEEVFSWM
                     EGFSVYGFSAAHAGSFAHLSYASAYMRRHYPAEFFCGLLDSQPMGFYSPRVLLNEARR
                     VGVGVLPPDIHLSERDFTVEEDGAALRPGLRYCKGLSERAISSILSERCKKPFSSVAD
                     LYGRTAVERDSLENLIRGGFLDSLAERFDRSRLLGETATLAKKRRPAGQSEIPLQHPA
                     SWWTMREKRSIEHLPLADTVKEKMEWEVLGLNVHRHPLTPYRDAFRRLGVVPSVEIMD
                     LPHGTRARAAGLIECLQNPPTKSGHRVYFLLIEDQWGLLQATIFRSVYERYGHILHGE
                     GAFLLEGKVEQTIKKGFAFLVHRVRSLQDVLIDARVPVPRVASAPGAFLRAGRRSRKA
                     S"
     gene            5108..5356
                     /locus_tag="AC16CM_01500"
     CDS             5108..5356
                     /locus_tag="AC16CM_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138531.1"
                     /translation="MSINLELPDEVVEALGPEPERSALEAVLLFLVSEDKMSVARAGE
                     ILHLDRTSSISWYTSHGFYYPDLSAEDLDEELRHARKP"
     gene            5372..5887
                     /locus_tag="AC16CM_01505"
     CDS             5372..5887
                     /locus_tag="AC16CM_01505"
                     /inference="COORDINATES: protein motif:HMM:NF023276.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3138532.1"
                     /translation="MQRPGHSRELAAFDTSSLIFLDLLGYIPLLRQLYRVLVPEAVSQ
                     ELSVRSGSPGSGVVSLSWVEHYIPDPKNVRRVEREPPTVGKGEKETIALGLELSCSIV
                     LDDKKARLRARRAGLEVTGTLGILLRLHRIGLTSRGIDEDLRLLEEAGMRLSPNLRRV
                     VIEVGKQEDGR"
     gene            complement(6074..7927)
                     /gene="ggt"
                     /locus_tag="AC16CM_01510"
     CDS             complement(6074..7927)
                     /gene="ggt"
                     /locus_tag="AC16CM_01510"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3138533.1"
                     /translation="MKRAFLVLMLTCILASCGAEPTEPEASFPEAGDARTTTEGQTTP
                     TAGTGKRATPEETTGREKTERANDADEPEIRQGPASSSTLTRPALGTNGMVSSAHPLA
                     TRAGLEVLEDGGNAYDAAVAVAASLGVVEPYMSGVGGYGAMVVYDAQSGSTRSLSAPT
                     RVPETLDPAVFRPSAPGYLQNRQGAPAISTPGAVHDWEALWKEYGQLRWERLLEPAVR
                     QAEEGFVLDEENAAWIASEFYAFPPHARRIYGKNGTPLLAGQRLVQEDLGRSLRQISE
                     EGADAVYGGGLGEAMASAAQRNGGFLTTDDLDANRPEWQEPTSVDYRGNEIVTTPTPT
                     TAWNALTRLGVMGQLDPEALGHNTVAYLDTYASVTRRAYYERSEYAADPEVARTPLDL
                     LLSEDYWEQEAAAVENPSASSSASASPVALDLTEQRGHTTHFVVADSGGNVVSMTQTL
                     GNIFGSKVMPEGTGIWLNNSISYSQFEPEGNPLDVYPSRQRLVGIAPTIIMRDDRPRI
                     ALGSHGGYYILQTTPQMIMNLIDFDMNVQQAIAAPRISFTAPSSFAVDAGMPASVQAG
                     LEAAGRGVYVDEYGLGNAHGLTIEYGRGKAGEPTSFTGGADPRAEGIATGY"
     gene            complement(8097..8951)
                     /locus_tag="AC16CM_01515"
     CDS             complement(8097..8951)
                     /locus_tag="AC16CM_01515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024783.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3138534.1"
                     /translation="MLDLFDLGEINRNFIEITEDLLDLFDSYWLAVLPFMKPSTLAMP
                     FVRMESEGFWHLVRREEGKPLFGGPESSVARLQEHVLGAYLDKSIYECLRVEAKRRIL
                     RSVLVGERYFSKEASGALLQQSFVNREGFLYSEKLLENPRDLEIRETLPLEEEYVPVR
                     DQGFRRAVVTAYSHRCALCGIRVRTLDGHTAVTAAHIVPWSETHDDRPANGMALCRMC
                     HWTFDEGLLGVSQAYEVIASKQLVISNNLPGYLTNLVGRGIVGPDQDLFWPDTASLRW
                     HHENVFRM"
     gene            complement(9534..10682)
                     /locus_tag="AC16CM_01520"
     CDS             complement(9534..10682)
                     /locus_tag="AC16CM_01520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3138535.1"
                     /translation="MASTMHEVQETSFADLPLSPQVYRAVVDQGWKTPTPIQALALPE
                     LLDGKDVVGLAQTGSGKTAAFAIPLIEGLDRKARGIQALVLAPTRELAAQAATEISNL
                     SRHEPIRPIVLCGGLSIGPQIKALEKRSARVVVGTPGRILDHLQRGTLRLGNVRYLVL
                     DEADRMLDMGFAPDVGRILSRTPEGRQTALFSATMPGAIRKMVKSHMRSPRYLMVESE
                     APTVDTVEQVYYRVDVRDKTQALRTLIDAEDNPLAIVFRRTKHGATKLHRQLEKAGYR
                     SALLHGGKSQSQRTKTLAGFARGRSRILVATNVAARGLDIPNVSHVVNYDLPEDTETY
                     VHRIGRTARAGKDGVAVTLVGEAELKDFDKIQRSLPVEVRNSKLPRSA"
BASE COUNT         1914 a         3355 c         3492 g         2027 t
ORIGIN      
        1 tcgcaggatg cgcggcatct ggatctgggt cagcaccgtc gacaacatga agaccgccgt
       61 cgcgagcccg gccccagagc taccaccacc ggcctcgtcg gcgtacagtg gcacgaccga
      121 gatcagcaac tggaaaccga agagcacggc gaaggcgatg agggtcaaca acacgaccgg
      181 cctggtcaac aaagaccccc gctccgtcgt cacggtcttt ttctccggcc cgttcacggc
      241 ccttacccta caacacgcga cgccccgggc atcgaggcag tttgcgggcg ggttcgaagc
      301 gggttttcca ggcgacgcgt ttttgggcgg ttcgcgaacc gcccctacag gttgtgtagg
      361 gtcaatgcct gcctctgggg gggtggacca cgtagaccac gcgtccctgt acctttacgt
      421 cctgggccgg tattaggagg tcctggtgat cgccgttttc gggtttgagc ctcagcatgt
      481 ctccgacttc gttcttttcg cggtagaggc gtttgacggt tacttcttcg ccgttcccga
      541 tcagggccgc aaccacggtt ccgtccgggg ggtcttcgtc ttcctctacg atcaggaggt
      601 ccccgtctgc tatgtgggcc ccgatcatgg actgcccaac gacgcgcaac aggtagcgtc
      661 gcctccccag acgagagccg acgagctcgg cggcgagcga gtaggcctcg tcttctacgg
      721 taacggcctc gagacctcgc cccgcggcga tccgacccag aagcggcatc tcccccgcgg
      781 cctcccagcc cttctccgtg agcctgatgc cccgcgcctt tccatccccc cgcccgacgt
      841 agccggcctc ctccagcctc ttgagatgct tgtaggccgt ctgcgagctc ctgaaaccga
      901 cggcctcccc caactcgcgt acgctcggcg gtcccttgcc tccagcctcc aggcgggcca
      961 gatgccgcag catctccatc ctggccttcc agttctcggg aaccatcctg gcctcctcgc
     1021 acgcctcttt ggagctctca gcaatcagcc ttcagctatc agcttttttg gctgactgct
     1081 gacagctaat agctgaccgc tataaaggac actttatatg attttgccct ggactgcaaa
     1141 caagtgtttg tggtagcgtg aaggaggaca tagagaaagc gagagcgccc ccaccccggg
     1201 cctcgcggat cggccggcgg ccaaccagca ccggtctgcg agggttaagg taggcgagga
     1261 cgcccgctcc ccggattata tccggtattg cgggtttccc atcccccggc tagggcgctc
     1321 acaggaggga ggagtatgga gacgagcgtc tcggagatac actgctaccc gctcaggccc
     1381 gagggcttcg aggagcctct gttcccgacc agggaatcga ggctggcggt tctggatcac
     1441 ctgctgaaat cttcctggct ctcgcgggcc tcggaggcgg agagtcgggg aggtcacgca
     1501 gcgcatgcct ggcgggagcc ggtagaggta gagaacagcg acgggcgcat gaaggtctcc
     1561 tgggcttctg gccgaaggca gtgccgcagg gagcggcgca tcgtcgaggt cctgggccgc
     1621 tggcgggagg tcagggcgtg gtgggacgag gggctggcga cggacaggga catcttcagg
     1681 gtgctactct ccggcggctc ggtggtcgac ctggcgcggg agaacggcga aggatggttc
     1741 ctcgtcggga cgctggattg accggggata acttcgtcca tttgcacgtc aggagtggtt
     1801 tctccttcgg gttcggggtc gcgaggcctg gggagctggt ggaggccgcc gccgggatgg
     1861 gcatgccggc tctggcgctc acggacaggg acgggctcta cggcatcccc cgctttctag
     1921 aggtggcggg cacggcgggc gtccttccca tcgtcggggt cgaggtctcg acggtggagg
     1981 ggcatatcgt gttgctagcc gagtccatgg agggctaccg ttcgctgtgc cggctcatca
     2041 cgcagtacag gacctcttcg gaggaccggc gcaggcccgt ctgtcccctg tccgcggtgg
     2101 gcgagcatgc ggagggtgtg atctgcctga ccggcgccgt ccctttcggg ctcctgccgc
     2161 gcctggtcct gggcggtcgc gggcaagacg ctatgagggt cctgcgctct ctgtccgggg
     2221 cgttcggcgg ggaccggctc tacgtcgagc tgaccgacga ccagacctcg gcagggaggc
     2281 ggaacgtggc gaagatggct gccttcgcgc aggacgctgg cgtacccgtc gtggccaccg
     2341 gtgaggtggc atacctctcg cccgccgacc acaggctaca cgaggtactg gtggcctcga
     2401 cgaaccttac ggcgctaccc ggcccggagt acaggccaac cgaccggctg cacctgaggt
     2461 ccgccgggga gatgcgtcgt ctgttcgcgg gctatcccca ggcccttgcg cacgcggccg
     2521 ggatcgccga gaggtgcgct ggaggggttg atctctccag cgcggtccac atgccgcagg
     2581 cgatcctgcc gccgggcaag acggcgggga aggtgttgct ggagctcacg atcgcggggg
     2641 ccaggagacg gtacaggaga gacagtcagg gaccggacgt agaggagatc aaagcccggc
     2701 taaggaggga gctatcgtgc atcgactctc tggggtttgc gccttacttt ctactcgcgc
     2761 acgaggcaag ggagatagcg aaatcgaaag gtatccccgt ctctggcagg ggaagcgccg
     2821 cgaacagcct ggtctcttat tgtcttggcc tgagcgggct cgagccgttc tcgaaccgtc
     2881 tgctcttcga gcgcttcatg cacgaggagc gcaaggaccc accggacata gacctggatt
     2941 tctgctccgt aaggcgggac gaggtgcgct tcgagatgat ccggcgctac gtgcggttcg
     3001 gggtggcgga agcggccacg gtccagacca tgtcgctgcg cggggccgtg cgggtcgcgg
     3061 cgcgggccct cggccattct ccggcggaga tcaacgatct ctgcaggcac gtcccaaccc
     3121 gctttcgtga ccggaacagg atctacgcgg ggctctcggg ttgggaagaa gcgctggccg
     3181 agccagcgat gcgcgggcat ccgctgcagg acacgcggag gtacgctctc ttgctggagc
     3241 tctcgacggg gctcgccgga cggacgaagg aggggggaac ccacagcggg gggatggtct
     3301 tcgggacggc ggagcgccac ctctcggagc tcgtgcccct cgagccttcc gggacggatg
     3361 gtctactgcg ctgccagtac gacaaggacg acctggaacg cgtggggttg ccgaagctgg
     3421 acttgctcgg cctccggatg cacacggcgc tgcacagggc cggcgtgctc gtatccaggc
     3481 gcctgggcag aaagatcgac ccctacgacc tgccgcccga ggacagggat acctatgctc
     3541 ttatcaggac gggacgaaac gccgggatgt tccagctgga gagcccgggg cagatgcatc
     3601 tgagccggag gctggggccc tggcggttca cggacctggt ggcccagatc tccctcttca
     3661 ggcccggtcc cgtgaggggg gacctcgtca ccccctacgt gatgaggaag aacggaaggg
     3721 agccgtacca ggcgatcctg cccgagctcg aagacgttct gcagcccacc tatggggtcc
     3781 tggtctacca ggagcaggtg ctcaccgtgg ccaacgccgt cgccggcttt accctggtgg
     3841 aaggggatct cctgcggcgc gccatgacca aggacagggg acctggcgcg atgaaggagc
     3901 tgcgccagaa gttcgtccgg cgggcggtgg agaacggcgt cccggcagcg gaggcggagg
     3961 aggtcttctc gtggatggag ggcttctccg tctacggctt ctcggcggcg cacgccggtt
     4021 cgttcgcgca cctctcctac gcgagcgcct acatgagacg ccactacccg gccgaatttt
     4081 tctgcgggct gctcgactcc caacccatgg gcttctacag cccgagggtg ctccttaacg
     4141 aggcccgcag ggtcggggtc ggggtcctgc caccagacat ccatctctcg gagagagact
     4201 tcaccgtgga ggaagacggc gcggcgctcc ggcccggcct ccgctactgc aaggggctct
     4261 cggagagagc catctcctcc atcctctccg aaaggtgcaa gaagccgttc tcctccgtcg
     4321 ccgacctcta cgggagaacc gccgtcgaac gcgactcgct ggagaacctc atcaggggag
     4381 ggttcctcga ctctctagcg gaaagattcg atcgttcgcg gctgctcggc gagaccgcga
     4441 ccctggcgaa gaagcgcagg cccgccgggc aatcggagat accgctccag cacccggcga
     4501 gctggtggac gatgcgggag aagaggagca tcgagcacct gcccctggct gatacggtca
     4561 aagagaagat ggagtgggag gtgctcggcc tcaacgtcca ccgtcaccct ctcacgcctt
     4621 accgcgatgc ttttcggagg ctgggggtcg ttccaagcgt ggagatcatg gaccttccgc
     4681 acggtacccg tgcccgcgcg gctggcctga tcgaatgcct ccagaacccg cccacgaaga
     4741 gcgggcaccg cgtctacttt ctgctgatcg aagaccagtg ggggctgcta caggccacca
     4801 tcttccgctc cgtctacgag aggtacggcc acatcctgca cggcgaaggt gctttcctcc
     4861 tcgaaggcaa ggtggagcag accataaaga aaggcttcgc cttcctcgtc caccgcgtga
     4921 ggagcctgca agacgtgctc atcgacgcca gggtgcccgt accgagggtg gcttccgctc
     4981 ccggtgcttt tctgcgggcc gggagaagaa gtaggaaggc cagctagtgt tgctcgactg
     5041 acaacccagg ctagcacacc gtgcctcatt tgcagcgcga gcccggcaaa agtataaaat
     5101 ataaaacatg agcataaacc tggaattacc agacgaggtc gtcgaagccc tgggaccaga
     5161 gcccgaacgc tctgcgctcg aagcagtgct tctatttctg gtctcagaag acaagatgtc
     5221 ggtggcccgg gctggcgaga tcctgcatct cgacaggacc tcatccatca gctggtacac
     5281 atctcacggt ttctactacc cggacctttc cgcagaagac ctggacgaag aactccgcca
     5341 cgcgcggaag ccatagaggc caagcatatc cgtgcaaagg ccaggtcact ctagagaact
     5401 ggccgctttc gacacgagct cgctgatctt cctcgatctc ctcggctaca tacccttgct
     5461 tcggcagctt taccgggtcc tggtacccga ggccgtgtca caggagctat cagtgcgttc
     5521 cggatctccg ggtagcgggg tcgtctcgtt gagctgggtg gaacactata tccctgaccc
     5581 gaagaatgtg cgccgggtgg aaagggaacc accaaccgtt ggcaagggcg agaaggaaac
     5641 catagcgctt ggcctggagc tttcgtgttc gattgtgctc gacgataaga aagcacgcct
     5701 gcgtgcccgt cgcgcagggt tggaggttac gggcacgctg gggatattgc tcaggctaca
     5761 ccgcattggt ctgacctcac gtggtatcga cgaggacttg aggctgctcg aagaagcagg
     5821 aatgcggctc tcaccaaacc tgcgaagggt ggtgatcgaa gtcggaaaac aagaagatgg
     5881 ccgatagcgc tgaagcaagc agctctccaa actttgcctt cctgatccaa tgcctgagag
     5941 gcctgcaaga ggtgctcata gcaggaaggc cgggtgagag aaggcaccag gctacaggtt
     6001 tcaggcttca ggtgaattac tgatgactgt gtctttctta cgcagtttca ctagttgtga
     6061 agaaccgccc ggttcaatag ccggtggcga tgccttcggc gcgtgggtct gcgccgccgg
     6121 tgaagctggt gggctcgccg gctttgcccc gtccgtactc gatggtcagg ccgtgggcgt
     6181 tgccgagccc gtactcgtcc acgtacacgc cgcggcccgc ggcctccagt ccggcctgga
     6241 cggaggcggg catgccggcg tcgaccgcga aggagctggg ggcggtgaag ctgatacggg
     6301 gggcggctat ggcttgctgt acgttcatgt cgaagtcgat gaggttcatg atcatctgcg
     6361 gcgtggtctg caagatgtag tagccgccgt gggagccaag ggcgattcgg ggtctatcgt
     6421 cgcgcatgat gatggtcggc gcgataccga caagcctctg gcgactgggg tagacatcga
     6481 gcgggttgcc ctcgggctcg aattgcgagt agctgatcga gttgttgagc cagatacccg
     6541 tgccctcggg catgaccttg ctcccgaaga tgttgcctag cgtctgggtc atgcttacca
     6601 cgttcccccc gctgtcggcc accacgaagt gcgtggtatg cccgcgctgc tcggtgaggt
     6661 caagcgccac cggagaggcg ctcgcggacg agcttgccga cgggttttct accgcggctg
     6721 cctcctgctc ccagtagtcc tcggagagca acaggtccag gggtgtccgc gctacctccg
     6781 gatcggcggc gtactcggac cgctcgtagt aggcccgccg cgtcacgctc gcgtacgtgt
     6841 cgaggtacgc cacggtgttg tggccgaggg cctcaggatc gagctggccc atgacgccga
     6901 ggcgggtaag ggcgttccag gcggtcgtcg gcgtgggagt agttacgatc tcgttacccc
     6961 tgtagtccac gcttgtgggt tcctgccact cggggcggtt ggcgtcgagg tcgtcggtgg
     7021 tcaggaagcc tccgtttcgc tgcgccgcgg aagccatcgc ctcgcccaga ccgccaccgt
     7081 acaccgcgtc cgcgccctct tcggagatct ggcgcaggga acgacccagg tcttcctgca
     7141 cgaggcgctg tcctgcgagg aggggcgtcc cgttcttgcc gtatatcctc ctggcgtgcg
     7201 gtggaaacgc gtagaattca gaggcgatcc aggcggcgtt ctcctcgtcg aggacaaacc
     7261 cctcctcggc ctgccttacg gcgggctcga ggagacgctc ccagcggagc tgaccgtact
     7321 ctttccacag ggcttcccag tcgtgcaccg cgcctggtgt cgagatggcc ggggcaccct
     7381 gcctgttctg cagataacct ggcgctgagg gtcggaacac ggctggatcc agcgtctccg
     7441 gcaccctggt gggggcgctc agggatctcg tactcccgga ctgcgcgtcg tagacgacca
     7501 tcgccccgta gccgcccacc ccgctcatat acggctccac cacgcccagg gaggccgcga
     7561 cggcgaccgc cgcatcgtag gcgttacccc cgtcttcgag tacctccaaa cccgcccgcg
     7621 tggcgagcgg gtgggcggag ctgaccatgc cgttggtgcc gagcgccggc cgggtcagcg
     7681 tgctgctgct cgctggcccc tggcggatct ccggctcatc cgcatcgttg gcccgttccg
     7741 tcttctcgcg tccggtggtc tcctcaggtg tggctctctt cccggtgccc gcggtggggg
     7801 tcgtctggcc ctccgtggtc gtacgtgcgt cgcccgcctc ggggaaagaa gcctctggct
     7861 ccgtgggctc ggcaccgcag gacgccagga tacaggtgag catcaagacc aggaacgccc
     7921 ttttcaaagc gtgcttacac catcgaagcg aaccgggcct ctaccaggaa ccacagcagg
     7981 acttgcggca tcgtcggcat cgttttcgcg tggtctctct tcggacatca gtcccatggt
     8041 ccgtagattc tatcctccac gctggggaca aagaggtaca aagccgggta ggcccgctac
     8101 atacgaaaca cgttctcgtg gtgccatcgc agcgacgcgg tatcaggcca gaacagatcc
     8161 tgatcaggcc caacgatacc gcgtcctacg aggttggtca ggtagccggg aaggttgttc
     8221 gagatcacaa gttgctttga agcgatgacc tcgtacgctt gtgaaactcc gagcaaccct
     8281 tcatcaaagg tccagtggca catcctgcac agcgccatgc cgtttgccgg ccggtcatcg
     8341 tgcgtctcgc tccagggaac aatgtgcgcc gccgtgacgg ccgtatggcc gtcgagggta
     8401 cggacacgaa tgccacacag ggcgcagcgg tgagagtaag cggtaacgac tgctcgtcgg
     8461 aaaccctggt cgcgaacggg cacgtattcc tcttcgagtg gcaacgtctc ccttatctcg
     8521 agatctctgg ggttctctag cagcttttcg ctgtagagga agccttctcg gttgacgaaa
     8581 ctttgctgca ggagggcccc gctagcttcc ttcgaaaagt atctctcacc caccagtacg
     8641 gagcgcaaga ttctgcgttt tgcttctacc cggaggcatt cgtaaatgga cttgtcgagg
     8701 taggcaccca aaacgtgttc ctgcagtctc gcaacagagc tttcgggccc accgaagagc
     8761 ggcttaccct cttcacgcct gaccagatgc cagaatccct cgctctccat gcgaacaaac
     8821 ggcatggcca gggtgctcgg tttcataaat ggcaagactg ccaaccagta actgtcgaaa
     8881 agatcgagga gatcttcggt gatctcgata aaattacgat tgatttcgcc aaggtcgaag
     8941 aggtccaaca cggaaagtag cagtattggc ttttgagggg cccggttcct caagcggtct
     9001 ggccacctgt tggcattctt gttggtcctc aaactagcga agagccgagc gcaaccttcc
     9061 gcgtgttcat cagcaggcac gcatccatat tagcggctac ctttgcaccg gagcaacgta
     9121 gcgttgctct tcgagtagat gatcgggccc cgcaacccaa ctgaatgatg gatcagccct
     9181 taacagcggc tccgttccct cgttgaggat gacgaggcag cgcgtgtggg gtgaagaagg
     9241 ccgactctag agagtgctgg cgcagaccag agagaccgag cagcagatcg ggcgtcaacg
     9301 gtgccggcac gaaggcccgc accaaacgac tctcccgtgg cggtgatctc ttaccgtacc
     9361 gccgttccac gcttcacact attctcactg gtgtaaacaa caccaaccac tatcaacgaa
     9421 tcgacgtcga ctcgttgata gtgcacccaa tcgtcttcgg actggcaacg aagtacagga
     9481 gagcctcaga ttgctcgcag cctgcatgcg cttacgttcg aacgatctcg gtatcaggcg
     9541 gagcggggga gcttactatt gcgtacttcg acggggaggg agcgttggat cttgtcgaaa
     9601 tccttgagtt cggcttcgcc cacgagcgtt accgccaccc cgtctttacc cgccctggcc
     9661 gtgcgcccta tccggtgcac gtacgtctcc gtgtcctcgg gcaggtcgta gttgacgacg
     9721 tgtgagacgt tggggatgtc gaggccacgc gccgcgacgt tggtggcgac caggatcctg
     9781 ctccggcctc gggcgaaccc ggcgagcgtc ttggtgcgct gcgactgcga cttcccgccg
     9841 tgcaggaggg ccgacctgta acccgccttc tccagctggc ggtgcagctt ggtcgccccg
     9901 tgcttggttc tgcggaacac gatagccagg gggttgtcct cggcgtcgat cagggtacgc
     9961 agggcctgcg tcttgtccct cacgtctacc cggtagtaga cctgctcgac ggtatccacc
    10021 gtgggcgcct cggactcgac catcaggtag cgcggggacc tcatatggct cttcaccatc
    10081 ttgcgtatcg cccccggcat cgtggccgag aagagggcag tctgcctacc ttccggcgtg
    10141 cgcgacagga tgcgtccgac gtcgggcgcg aagcccatgt cgagcatcct gtctgcctca
    10201 tccaggacca ggtagcgcac gttgccgagg cgcagcgtgc cccgctgcag gtggtcgaga
    10261 atgcgacccg gcgtgccgac cacgaccctg gcggaacgct tctcgagggc tttgatctgg
    10321 ggtcctatgg acaagccccc gcagaggacg atgggacgga tcggttcgtg gcgcgagagg
    10381 ttcgagatct ccgtcgcggc ctgcgcggcg agctcacgcg tcggcgcgag cacgagcgcc
    10441 tgtatacccc gcgccttcct gtctagaccc tcgatcaggg gtattgcgaa agcggcggtc
    10501 ttgccgctgc cggtctgcgc gagcccgacc acgtccttgc catccagaag ttcgggcaac
    10561 gccagagcct gtataggcgt cggggtcttc catccctggt cgacgacagc cctgtaaacc
    10621 tgtggggata ggggcagatc cgcgaaagag gtctcctgga cctcgtgcat agtgctagcc
    10681 aaaatgtgct gttcctctct ctgtagctgt gctgtggtcg ccaccataag gagcgtacgc
    10741 cgcgtactac cactcgacgg gagaggacag gcacaagaca caaaaaaa
//
LOCUS       DBNGVD010000057        16320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_737997, whole genome shotgun sequence.
ACCESSION   DBNGVD010000057 DBNGVD010000000
VERSION     DBNGVD010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16320
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_737997"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..811)
                     /locus_tag="AC16CM_01525"
                     /pseudo
     CDS             complement(1..811)
                     /locus_tag="AC16CM_01525"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
     gene            complement(823..1038)
                     /gene="rpmE"
                     /locus_tag="AC16CM_01530"
     CDS             complement(823..1038)
                     /gene="rpmE"
                     /locus_tag="AC16CM_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3138536.1"
                     /translation="MKTGIHPEYTTTLVRCMCGNEFETRSTAGDTITVDLCSECHPFY
                     TGKQRIVDTGGRVQRFQNRRQASQNRK"
     gene            complement(1185..2711)
                     /gene="rho"
                     /locus_tag="AC16CM_01535"
     CDS             complement(1185..2711)
                     /gene="rho"
                     /locus_tag="AC16CM_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3138537.1"
                     /translation="MTELSTLERKRLSDLHQIASQLGIKQYRKYRKPELAEMIYKVAT
                     EQAASQQESPTNGASESPAAGASDSAATTAEAPQTNGAAAKAPEPVKNEATTEAPAEA
                     KAAAEAPAPEHRRQQRGDERRRQDRKQDRGGRGGGDEQVQSGILDVLPDGFGFLRTGG
                     YSQSKGDVYVSTSQIKRFNLRRGDYITGQIRPAREGEKYPAMVRIETVNGQEPQKGRW
                     RPNFDDLTPLYPMERLRLEWKPNDIAPRVIDLVAPIGKGQRGMVVSPPKAGKTTILKQ
                     IAQSIAANYPETRLFVVLADERPEEVTDWERSVEEAEVVASTFDQPADNHIAVAELVL
                     ERVKRLVEEGEDVVVLLDGITRLSRAYNLAAPASGRILSGGVDSAALYPPKKFFGAAR
                     NIENGGSLTILATALVETGSRMDEVIFEEFKGTGNMELKLDRKLANRRIYPAINIEDS
                     STRKEELLMEPAEAQRVWQVRSILNALDAGQKIELLIQKLKETRTNPEFLRELQRVRG
                     "
     gene            complement(3213..4541)
                     /locus_tag="AC16CM_01540"
     CDS             complement(3213..4541)
                     /locus_tag="AC16CM_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HIR3138538.1"
                     /translation="MRVLFLIDGEHYPPVVLDAMRSVQDSLGAVGVAAAFLGGTEKIK
                     AGTDYGVPLVTAPDPVSAVEQALNGYGVDAVVDLSDEPVIGYKERMRIASLVLFAGAR
                     YVGSDFELRPPDFRAVSTKPSLAVIGTGKRVGKTGVSGYLARLLSDEGFDPGVVSMGR
                     GGPPHPEVIEGHKLEVGSEYLLEALARGAHAASDYYETAALSRVTTVGCRRCGGGLAG
                     EPFVSNVVEGATIANGLDTRVTVFDGSGAAMPPVRVQKRVLVAGAHQDPEYIVGFLGT
                     YRLLVSDLVLLTMSEEPMAGQEKVDGLVRRIGEVRPDLTVIPTVFRPRPVGRIEGLRI
                     GYVSTAPPAVLDKLSRYLEDHHGCEVVAASGNLSDRARLAADLDEMTGVEAYLTEIKA
                     AAVDVVTRRGAEEGKPVLYCDNDPVGEGLDARLLALAREAMAEQAAQDGG"
     gene            complement(4549..5355)
                     /locus_tag="AC16CM_01545"
     CDS             complement(4549..5355)
                     /locus_tag="AC16CM_01545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3138539.1"
                     /translation="MGTQDRMVICQISDIHCGSEYFIPDLLERSILEINDLNATAVVV
                     SGDLTDAGYRQEFEEAAEYLRRLTCDRVMVIPGNHDSRNVGYIHFERLFGERYSVMDF
                     DDAVMVGVDSSEPDLDDGRVGREHYPFIHETFAGAENKLKIFVIHHHLIPIPSTGRER
                     NIIFDAGDVLELLADTEVDLVLSGHKHVPYSWRLEDMFFVNAGTASTTRLRGNTRPCY
                     NIIEIEDGRVQVFRKYPFKDRESIVDFDAATRKYQHYEKIQNDPQPRTMG"
     gene            complement(5400..6311)
                     /locus_tag="AC16CM_01550"
     CDS             complement(5400..6311)
                     /locus_tag="AC16CM_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3138540.1"
                     /translation="MNGNQHERDPNEIRLVTYLSPSIPRAFFEALADHLRTSLGNRRV
                     SLHIERNVSGPRRGHECSTFGDRVDVAFLCAPSFAWLRELHPPPAELLGVAPVFDDER
                     NGGRPVYFCDVIVRNEAPIHAFSDLLGGRWAYNDESSLSGYYGLLHKLAESGRDESFF
                     DSVACSGSHIDSIEAVLGDEADAASIDSNVLRIRMRETPALGHRLRVIESWGPYPIQP
                     VVVNSNLDRDLKDQLRRAFLTTNENERTRRILKRFGLSHFTTVNGESYNLTAHQNLAT
                     LLTNPARHHKNSAESEAKPKRADKRRR"
     gene            complement(6347..7984)
                     /gene="argS"
                     /locus_tag="AC16CM_01555"
     CDS             complement(6347..7984)
                     /gene="argS"
                     /locus_tag="AC16CM_01555"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3138541.1"
                     /translation="MSFEERLAQAVREAVRQAYGIELNGIHVERPNDPSHGDFATNVA
                     LANARVFRRNPREVAENLVGALDAPFVREAGVAGPGFVNFWLSREALWDGVSTILREH
                     DAYGRQEVSGDPVLLEFVSVNPTGPMHVGHGRQAAYGDSLARIMETSGRNVSREYYFN
                     DGGNQIRIFGESIATRYAGLYGETWPTSDAEPAYGGDYTQEIAEDIAEEHGRRLLEID
                     SEEALREITAFATRWCMDDIKRTLARVRVHFDTYFNEKDLYESGATGQIIQELAEGDN
                     LYDGEGAVWLATSRFGDDKDRVLVKSDGSYTYMVPDLAYHRDKWERGFRTAIDVLGAD
                     HAGYPPRVRAGLVALGLPREFLDVELVRLVKLVQEGEQVKFSKRAGNIVTFDELLDAV
                     GEDVARYFYVRSSHKTEMNFDLDLAIKQSDENPVFYVQYAHARISSIFGRAGTNPDEV
                     GELPPGELAPEERLLVLELLDFPRVIENCAARREVHPLPTYLETLATRFHQFYTVHRV
                     LVENDDVRTRRLALCAATQTVLRSGLGLLGVDAPEKM"
     gene            complement(7981..8202)
                     /locus_tag="AC16CM_01560"
     CDS             complement(7981..8202)
                     /locus_tag="AC16CM_01560"
                     /inference="COORDINATES: protein motif:HMM:NF022659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HIR3138542.1"
                     /translation="MRVQIDRFEDNGWAVLLLYPDGRRSFDVPRELLPEEVSAGDVFE
                     VRFDYDREETLRLAEENRRLLDDLLRGGS"
     gene            complement(8297..8770)
                     /locus_tag="AC16CM_01565"
     CDS             complement(8297..8770)
                     /locus_tag="AC16CM_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3138543.1"
                     /translation="MIRRCTIVCVGKPRGWAAEASEDYAKRLRRYFPVDIIEVAEEDI
                     NRRSRDEVLVREGDRILKRLPPDAYVVCLDREQGKQLPSEKLAGRLEDLGVSGHSHVA
                     FAIGGALGLSPEILRSSRELWSFGPITLPHALARVVLLEQFYRATKIERGEKYHW"
     gene            complement(8767..9579)
                     /locus_tag="AC16CM_01570"
     CDS             complement(8767..9579)
                     /locus_tag="AC16CM_01570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3138544.1"
                     /translation="MLSSGSSGNATYMETDTGGLLVDAGLSRRRIESLLGGIGRNLGD
                     VRAILLTHGHSDHTCGVRSILRGRPVPVYAAPGVGESLGASAVSPEEPCVVGGLTVTF
                     FPVPHDSLTYGVRISDEASTVAMATDLGEAGIDVLRCMRGADAVVLEANHDHDWLRRG
                     PYSSSLKRRIASRHGHLSNRQTGEAAVALAPYGLKDLVLAHLSKTNNSPTRACGTVYG
                     ALREAGHGGVRVRAAIAGHPTPWIEAGTPIEVSEHVYRYEENDAAGQLFGIE"
     gene            complement(9654..10853)
                     /locus_tag="AC16CM_01575"
     CDS             complement(9654..10853)
                     /locus_tag="AC16CM_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR3138545.1"
                     /translation="MDTAFVHRSDEENSRGDYTYLSDPSIDGDPNAVVLVSSLPEQGN
                     DTEGTYDHNVGVWYEPRAQRWAVFNQDRAAVPDGTTFQVVIPGGAEKLVHRAGPDNTE
                     DNSTSLDDPLVNGKPEAILSVTQNWNPGGGSGTYNDHPVGVRYDSGSQRWEIYNTDGS
                     AMPKGAAFNVAVSGIDLEAGGDAEDQAAEPSEEQDSQTSTEAVLPEDVLDEKDLEKSP
                     PKYEDWHREGSDSIEVETSDTSAGTIPAVKPFNLGRDPGGPDDKTLYLTVPDLGLTDV
                     PVYNSVEDEKLEESVVHVPATGFPWQDGANTYIAGHRLGYEGTGSYLIFYYLDQLGEG
                     DEIKLEDEAGNTYKYRVTGQKIVDPDNVEVMEPQDGKSIVSLQTCTLPDYEQRLIVQG
                     ELVEEST"
     gene            complement(11097..11780)
                     /locus_tag="AC16CM_01580"
     CDS             complement(11097..11780)
                     /locus_tag="AC16CM_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3138546.1"
                     /translation="MDKINVPGAHELARPRTRRDFFKALAVAGAGATAASTLFAGRAS
                     AKTDDVDIANFALTLEYLEADFYALALDSGTLSGDALGVVQNLESHESQHVDALVGLL
                     EGVGATPVEKPTFTFPADATASEAAIHQTAATLEPVGVGAYLGAAPLISSPDILAAAG
                     SIAGVEGEHVVAINWLLGVVPYANEAFPKALTLDEVSAAVAPFMGGKMLSTGGPKLSP
                     EEMYRLAHV"
     gene            complement(11799..12458)
                     /locus_tag="AC16CM_01585"
     CDS             complement(11799..12458)
                     /locus_tag="AC16CM_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3138547.1"
                     /translation="MSGVESRVPIGMDGETSRRNFLKTAALTGAALSAMGIGGFGVAV
                     RRAEAEGEYETYEGLGDAAIVAFAYQLELLEGTFYAEGVNAGIFKDAFLEQIVAIRDH
                     EMAHADALATTLGQLGAEVPATPEFTFPSGTFSDAATFLELAATFEPVGIGAYQGAAP
                     ALKSKDILAAAISIHNAECQHRCAINILNLINPPNDLAFEVALPLQKVQAAVEPFGIT
                     G"
     gene            12656..13243
                     /locus_tag="AC16CM_01590"
     CDS             12656..13243
                     /locus_tag="AC16CM_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3138548.1"
                     /translation="MADRGKQYLILADEDLISLLEASDSEAFATLYDRHSRAAFSLAY
                     RMMGDRQAAEDLAQDAFLKVWRGAGSYRAERGSVRTWILSIVHNRGIDQLRSHASRRR
                     TQDKIEASAPRSQPSEAFAETWRNSQREQVREALNTLPREQLKILELAYFSGYTHAEI
                     SELLSLPLGTVKGRMRLGLKKIRDFFESRGAAVPR"
     gene            13240..14007
                     /locus_tag="AC16CM_01595"
     CDS             13240..14007
                     /locus_tag="AC16CM_01595"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3138549.1"
                     /translation="MNEMNNERFEDLKDAYVLDALAEEEKRSFEEFLAAHPERQAEID
                     ELGAVAGLLAFSPEVQEPPPDLRTRIMNVVAAEAAPRRERQGSVLGGLWGYLGARGLA
                     LGAAALLLIGLLSWNLLLQDQVDDLQGQVQSSQGQVEDLQAQVREAQAQQTQTVQLSG
                     TWTTQGADAEVASISGDRIILVADDLPAIPEGQTCQIWVIKGDVPEPSGLFQPGGTET
                     AAPITTPIKKGDTIAVTVEPAGGSEQPTTDPVLSAEL"
     gene            14146..15018
                     /gene="uvsE"
                     /locus_tag="AC16CM_01600"
     CDS             14146..15018
                     /gene="uvsE"
                     /locus_tag="AC16CM_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HIR3138550.1"
                     /translation="MIRFGYPAQNLTLPATTNRTLRLANIPHVEKLRGLVRGNLADLR
                     SILRWNAEHGIALFRMGQDLIPFASHPAFPYDWQDEHGEELRSIGALSRDLGIRLSMH
                     PGQYINPGSPRPEVVERSLWELEYVASVFDLLGNRDSVAVLHMGGAYADRAGSAARFV
                     EALRGESRILRYLALENDERVWTVAEVVEASRLLGIPTIMDAFHHGLNPGDLTLEGAL
                     DAALPTWEIRGVRPKLHLSSQDPDKRPGAHAPTVEERDWQALLAALGGRDADVMVEAK
                     SKERALASLGVKMG"
     gene            15056..15475
                     /locus_tag="AC16CM_01605"
     CDS             15056..15475
                     /locus_tag="AC16CM_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3138551.1"
                     /translation="MHKNPDRQEIRALLDRSRTVAVVGLSDSPYRTSHAIAGALQGFG
                     YRIFPVNPNLSGPVLGELPYDRVVDVPTPVDVVDVFRRSEKVMPVAEDAVAAGAKVLW
                     LQSGVVNEEAAAYATEQGLTVIMDRCIKVDYASLIGR"
     gene            15547..16008
                     /locus_tag="AC16CM_01610"
     CDS             15547..16008
                     /locus_tag="AC16CM_01610"
                     /EC_number="4.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061929 -
                     gamma-glutamylaminecyclotransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR3138552.1"
                     /translation="MLTIFVYGTLKRGERNHGYCSGALRVEEGAVRGELYDLPRFGYP
                     ELIVPEESVRAFGTDDYLGDAELQARLSPGSPSPPESPLVYGEILVFDDPGSRLPAID
                     RLEGFDPAEDSSRYRRVLLPVELEEGPRLLAWAYAVSEPWGAHVPDGRWPP"
     gene            complement(16056..>16320)
                     /locus_tag="AC16CM_01615"
     CDS             complement(16056..>16320)
                     /locus_tag="AC16CM_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138553.1"
                     /translation="TVPTAGTSERPGGFGETSESPGADSLSAGTSPESTGPFTDEAPQ
                     STPTIRETEETETFREGGRTKIRRRVIREEIIEEDQDAPTSNP"
BASE COUNT         2765 a         5268 c         5212 g         3075 t
ORIGIN      
        1 ctgagggctc gcgggtggtc agcttctgga gctggaggcc cggccagatc aggaccttca
       61 ccagcgggtt ctcctggttc ttcgcgctcc acatgatgaa ctcgaacgcg attcccgcga
      121 ctagcggaat cacgacgatg cggctcgcca gcatgtagag ccacccctcg acaccgagca
      181 gcgagaagat caggatggag agcaccagga cgtataggac aaagctcgtc ccgcagcgca
      241 cgtggctcgt gtcaaacttg cgggcgttct cgggtaccag ctcctcaccc cgctcgtaga
      301 ccttgatcgc ctagtgctcg gcaccgtggt aggcgaagaa gcgtttgatg tctttgctca
      361 ggaacgagat acccactata taagcgacga agatcgctat tctgataacg ccctcgacga
      421 ggttgaatat tatggggttc tcgatgctca tcccgatcac cgaccccaac cccttggtcc
      481 caagcaccgg ggccacgaga aacagcccga acccgagcac catcgcgaag atgagggtga
      541 aggcgatctc ttttctggag agctcctcct cttcgccgag ggcgatgttc gtggagagcg
      601 tgagcgcctt catgccgagc cacaacgtct ccccgaggga gagcgcccca cggatgattg
      661 gaatacggaa cagcttgctg cggcgcgtga cggagctgaa cggctccgcc cgcaggtcca
      721 cgtcgcccga aggagtacgg acggccatcc cccagaagtt gggagagcgc atcatcacgc
      781 cctccatgat ggcctgtccg ccaaccctca tgacgccttg ccctacttcc tgttctggct
      841 ggcctggcgg cggttctgga accgctgcac ccgaccgccg gtgtcgacta tgcgctgctt
      901 gcccgtgtag aacgggtggc actcggaaca gaggtcgacc gtgatggtgt cccccgccgt
      961 cgagcgcgtc tcgaactcgt tgccgcacat gcagcgcacc agggtagtcg tgtactccgg
     1021 atgtatgcca gtcttcatct tcgcctcctg cttttgacgt tactcgaata ttctaccgca
     1081 gccgccctgc aattcagggg agtggggagc agagaaaggg agcagggctt cgttctcccc
     1141 tactccccgt ctactatacc ctgcccgccg gccgcgaagc ggccctatcc gcggacgcgc
     1201 tgaagctcgc gcaggaactc ggggttcgtg cgggtttcct tgagcttctg aatgagaagc
     1261 tctatcttct ggcccgcgtc cagggcgttc aggatgctac ggacctgcca gacccgctgg
     1321 gcctcggccg gctccataag cagctcttcc ttacgcgtcg aagagtcctc gatgttgatg
     1381 gccgggtaga tcctgcggtt cgcgagcttc ctgtcgagct tgagctccat gttgcccgtg
     1441 cccttgaact cctcgaagat gacctcgtcc atcctgctgc cggtctccac gagcgcggtc
     1501 gcgagtatgg tcagcgaccc accgttctcg atgttgcgcg ccgcgccgaa gaacttcttg
     1561 ggcgggtaca gcgcggccga gtcgacacca ccggagagga tgcgtcccga ggcgggggcc
     1621 gcgaggttgt aggccctgga gagcctcgtt ataccgtcca gcagcaccac cacgtcctcg
     1681 ccttcctcga ccaggcgctt gacgcgctcc aggacgagct cggcaacggc gatgtggttg
     1741 tccgcgggct ggtcgaaggt cgaggccacg acctcggcct cctccacgct cctctcccag
     1801 tccgtgacct cctcggggcg ctcgtcagcg agcaccacga acagcctggt ctccgggtag
     1861 ttggccgcga tggactgggc aatctgcttg aggatcgtcg tcttcccagc cttgggcggc
     1921 gagaccacca tgccacgctg gcccttgccg atgggcgcga caaggtcgat gactcgcggg
     1981 gctatgtcgt tcggcttcca ctccagacgc agccgctcca tggggtagag cggggtcaga
     2041 tcgtcgaagt tggggcgcca gcggcccttc tggggctcct ggccgttcac cgtctcgata
     2101 cggaccatcg ccgggtactt ctcgccctca cgggccgggc ggatctgacc cgtgatgtag
     2161 tctccacgcc tcaggttgaa gcgtttgatc tggctggtcg atacgtagac gtcgcccttg
     2221 ctctggctgt agcccccggt cctaaggaac ccgaagccgt cgggaaggac gtcgaggatg
     2281 ccgctctgca cctgctcgtc accaccgccg cgaccgcccc tgtcctgctt gcgatcctga
     2341 cgacgccgct cgtcgcccct ctgctgcctg cggtgctcgg gggccggcgc ctcggccgca
     2401 gccttggcct ccgcgggggc ctccgttgtg gcctcgttct tcacgggctc gggggccttc
     2461 gccgcggcgc cgttggtctg cggtgcttcg gccgtggtcg cggcgctgtc ggaggcgcca
     2521 gcggcggggc tctctgaggc gccgttcgtg gggctctcct gctgcgaggc ggcctgttcg
     2581 gtggcgacct tgtagatcat ctcggccagc tcgggcttgc ggtacttgcg gtactgcttg
     2641 atcccgagct gcgaggcgat ctggtgcaaa tcgctcagcc gcttgcgctc gagggtcgaa
     2701 agttcggtca tgtttgtact ccacctctcc tgtatacgat gcgtaaaacg tcgtccgtct
     2761 ggtttcgttg cggatctgtc aaagctggaa gccccgccac ccggcttcgg gctctagggc
     2821 gtggatggtg tccacgaatc ttaccacacg ggacccggta gacatagcga ccttatgcgt
     2881 ccgcgcggca ccccgggaac acctctcccg aaaagcctcg gtggcgaacg tgtaggcccg
     2941 atccgagtca cctggccctt cgagcccggc caccttcgag gcgacccgcc cggttacggc
     3001 ggcgtactcc acggcagccg agactacggt tcccgaacgg gaatcggtaa cgctaatggg
     3061 ttctcaccga acctccataa cccgagatgg accgcaaaca acgggctact tctcccgagg
     3121 ttgggtctgc gtcgaaacgg tgcggactgg ctccttatcc tgagcggaaa ccaggttacc
     3181 tgacgtaaat attatacacc aggcgtcagg cgtcaaccac catcctgagc tgcctgctcc
     3241 gccatcgcct cccgcgccag tgcgagtagc cgcgcgtcga ggccctcccc cacgggatcg
     3301 ttgtcgcaat acaggacggg cttcccctcc tcggcccccc gccgcgtaac gacgtccacg
     3361 gcggcggcct ttatctcggt tagataggcc tcgaccccgg tcatctcgtc gaggtcagcg
     3421 gccagacggg cccggtcgga gaggttgccg gatgcggcga ccacttcgca gccgtggtgg
     3481 tcctcgagat acctggagag cttgtcgagg acggcaggag gggctgtcga gacgtagcct
     3541 atcctgagac cttcgatcct ccccaccggt cgtggcctga agaccgtggg gatcacggtg
     3601 agatccggcc ttacctcccc gatcctccgc acaaggccat cgaccttctc ctgccccgcc
     3661 atgggctctt cgctcatcgt gagtaggacc aggtcggaga cgagcagacg gtaggtcccg
     3721 agaaagccga cgatgtactc cgggtcctgg tgggccccgg cgacgagcac gcgcttctgt
     3781 acccggacgg gcggcatcgc ggcccccgac ccgtcgaaga ccgtcacgcg cgtgtcgagc
     3841 ccgttggcga tggtagctcc ctccaccacg ttcgagacga acggctcgcc ggcgaggccg
     3901 ccgccgcacc tgcggcatcc gacggtggtc accctgctaa gggccgccgt ctcgtagtag
     3961 tcgctcgcgg cgtgcgcccc gcgggccagc gcctcaagga ggtactcgct gccgacctcc
     4021 agtttgtggc cctcgatcac ctccggatgc gggggtccgc cacgtcccat cgagaccacc
     4081 ccggggtcga aaccctcgtc ggagagtaga cgggccaggt aaccggagac gcccgtcttg
     4141 cccacccgct tgccggtccc tatcacggcc agcgacggct tggtagagac ggcgcggaaa
     4201 tccggcggcc tgagctcgaa gtcgctgccg acatagcggg cgccggcgaa gaggacgagc
     4261 gaggctatcc tcatccgctc cttgtagccg atcacgggct catctgagag atcaacgacg
     4321 gcgtccaccc catagccgtt cagggcctgt tctacggcgg agacggggtc gggggccgta
     4381 accaggggaa ctccgtaatc ggtgccggcc ttgatcttct cggtcccgcc gaggaaggcc
     4441 gcggccacgc ctacggcccc gagagagtcc tgtacgctac gcatcgcgtc aaggaccacg
     4501 ggaggatagt gctcgccgtc gatcaggaag aggaccctca aggtttctct agcccatagt
     4561 ccggggctgg gggtcgttct gaatcttctc gtagtgctgg tacttgcggg tcgccgcgtc
     4621 gaagtcgacg atcgactccc tgtccttgaa cgggtatttg cggaagacct gcacccgtcc
     4681 gtcctctatc tctatgatgt tgtagcaggg acgggtgtta cccctgaggc gcgtcgtcga
     4741 ggcggtaccg gcgttcacga agaacatatc ctccagcctc caggagtaag gcacgtgctt
     4801 gtgaccggag aggacaaggt ccacctccgt gtcggccagt agctccagga cgtcgcccgc
     4861 atcgaagatg atgttgcgct cgcgtcccgt gcttgggatg gggatgaggt ggtggtggat
     4921 gacgaagatc ttgagcttgt tctcagcccc ggcgaaggtc tcgtggataa aggggtagtg
     4981 ttcgcgtccg acccgcccgt cgtcgaggtc gggctccgag gaatctaccc cgaccatgac
     5041 cgcatcgtcg aagtccataa ccgagtagcg ttccccgaac aggcgctcga agtggatgta
     5101 tccgacgttg cgcgagtcgt gattgcctgg gatgaccatg acccggtcac atgtcaggcg
     5161 cctcaggtac tccgccgcct cctcgaactc ctgacgataa ccagcgtccg tgaggtcacc
     5221 cgagacgacc acagcggtcg cgttcaggtc gtttatctcc aggatggaac gctccaggag
     5281 gtcggggatg aagtattcgc ttccgcagtg gatatcgctg atctggcaga taaccatccg
     5341 gtcttgcgtg cccattagct ccccgatccg acgccctcgc gtatagtagc acgctccggc
     5401 taacgcctac gtttgtcggc acgcttcggc ttcgcctcgc tttcggctga gttcttgtgg
     5461 tgtcttgccg ggttggttag tagtgttgcc aggttctggt gggcggtgag gttgtagctt
     5521 tcgccgttca cggtggtgaa gtggctgaga ccaaaacgct tcaggatccg gcgcgtgcgc
     5581 tcgttttcgt tggtggtcaa gaaggccctg cggagctggt ctttgagatc tcggtcgagg
     5641 ttggagttga ccactactgg ctgtatcggg tagggccccc aggattcgat gacgcggagc
     5701 ctgtggccca gggccggtgt ctctcgcatc cgtatgcgca agacgtttga atcgatcgag
     5761 gcggcatcgg cctcgtctcc gaggactgcc tctatggagt cgatatgcga accggagcag
     5821 gccacgctat cgaagaagct ctcgtctcgg cctgattcgg ccagtttgtg taggagcccg
     5881 tagtagccgc tcagcgaact ctcgtcgttg tacgcccacc taccccccag aaggtcggag
     5941 aaagcatgga tcggggcctc gttgcgcacg atcacgtcgc agaagtagac cggccttccc
     6001 ccgtttctct cgtcgtcgaa cacgggtgct acgccgagca gttctgcggg cggtgggtgc
     6061 aactcgcgca gccaggcaaa cgaaggggcg cacagaaacg ccacatcaac cctgtctccg
     6121 aaggtggagc actcgtgtcc tctcctcggg cccgagacgt ttctctctat atggagcgag
     6181 acccgtctgt ttccaagaga cgttcgaagg tgatcagcca gtgcttcgaa gaaggcccgc
     6241 gggatgctag gcgagaggta ggttaccaac cggatctcgt tcggatccct ctcgtgttgg
     6301 ttaccgttca acgcctacct gacagagcac cagacgtcgg gagcgcctac atcttctctg
     6361 gcgcgtcgac gccaagcaga ccgaggccgc tgcggagcac ggtctgggtg gccgcgcaca
     6421 gggcgaggcg gcgggtgcgg acgtcgtcgt tttccacgag gacccggtgg acagtgtaga
     6481 actggtggaa gcgggtggcc agcgtctcca gatatgtcgg gagcggatgg acctcccgtc
     6541 tggcggcgca gttctcgatc acacgaggga aatcgagcaa ctcaagcacg agcagacgct
     6601 cctcgggcgc gagctccccg gggggaagct ctcccacttc gtctgggttc gtcccagctc
     6661 gcccgaagat gctggagatc ctggcgtggg cgtactgcac gtagaagacg gggttctcgt
     6721 ccgactgctt tattgccagg tcgaggtcga agttcatctc cgtcttgtgg gaggagcgca
     6781 cgtagaagta gcgcgccaca tcctccccca ccgcgtcgag cagctcgtcg aaggtaacga
     6841 tgttgccggc ccgcttgctg aacttgacct gctcgccttc ctgaacgagt ttgacgagcc
     6901 tcacgagctc gacatccagg aactcccgcg gcaacccgag cgcgaccaga ccggccctga
     6961 cgcgtggcgg gtatcctgcg tgatccgcgc cgagcacgtc gatggcggtc ctgaaacccc
     7021 gctcccactt gtcgcggtga tacgccagat cgggaaccat gtaggtataa gagccatcgc
     7081 tcttgaccag caccctgtcc ttgtcgtcac cgaacctgga ggtagcaagc cacacagccc
     7141 cctcgccatc gtacagatta tcgccctccg caagctcctg tatgatctgc ccggtcgcac
     7201 cagattcgta gagatctttt tcgttgaagt acgtgtcgaa atggacccgc acgcgggcca
     7261 gcgtgcgctt gatatcgtcc atacaccagc gggtcgcgaa cgcggtgatc tcgcgcaaag
     7321 cctcttcgct atctatctcg agcagacgcc tgccgtgctc ctcggcgatg tcctcggcta
     7381 tctcctgcgt gtagtccccg ccgtaagcag gctcagcatc ggaggtgggc caggtctcgc
     7441 cgtagaggcc agcgtatcgg gtggcgatcg actcgccgaa gatccggatc tggtttcctc
     7501 cgtcgttgaa gtagtactcg cgcgagacgt tcctgcccga ggtttccatg atcctcgcca
     7561 gcgagtcccc gtaagccgcc tgccgcccgt gtccgacgtg cattggcccc gtcgggttga
     7621 cgctgacgaa ctcgagcaaa acagggtccc ccgaaacctc ctgccgtccg tacgcatcat
     7681 gctcgcgcag tatggtggag accccatccc agagcgcctc ccgggagagc cagaagttga
     7741 cgaacccggg tccggcaaca ccggcctccc tcacgaacgg cgcgtcgagc gccccaacga
     7801 ggttctccgc cacctccctc gggttacgcc taaaaaccct ggcgttggcg agggcaacgt
     7861 tggtcgcgaa gtcgccgtgg ctcgggtcgt tgggccgctc gacgtggatg ccgttgagct
     7921 cgatcccata ggcctggcgc acggcctctc gcaccgcctg ggccaggcgc tcctcgaagc
     7981 tcatgacccg ccccggagca gatcgtccag aagccgccgg ttctcttcgg caaggcgaag
     8041 cgtttcctcc ctgtcgtaat cgaagcgaac ctcgaaaaca tcccccgccg agacctcttc
     8101 gggcagcaac tcgcggggca catcgaagct ccgccgcccg tcaggataga gcaagagcac
     8161 cgcccacccg ttgtcctcga acctgtcgat ctgtacgcgc atgccagtat tctagcagtc
     8221 agctttcagc agtcagccat caggttcttg tttttggctg actgttgtcg gctgatagct
     8281 gcggagcgaa gcggagctac cagtgatact tttcgccgcg ctctatcttg gtggcgcggt
     8341 agaattgctc caggagcacg acccgggcga gggcgtgggg tagcgtgatc gggccgaagg
     8401 accagagctc ccggctgctt cgcagtatct cgggcgagag gcccagcgcg ccgccgatgg
     8461 cgaaggcgac gtgggagtgg ccggagacgc cgaggtcttc gagtctcccc gcaagcttct
     8521 cggaaggaag ctgcttgccc tgctccctgt ccaggcagac gacgtaggca tctgggggca
     8581 ggcgtttcag gatgcggtct ccctctcgga cgagaacctc atcgcgggag cgtcggttga
     8641 tatcttcctc cgcgacttcg attatatcta ccgggaagta gcggcgaagc cgttttgcgt
     8701 agtcctcgga ggcttccgcg gcccagcccc ttggctttcc cacacagact atggtgcagc
     8761 ggcggatcat tcgatgccga agagttgtcc cgcggcgtcg ttctcttcgt agcggtagac
     8821 gtgttcggaa acctcgatgg gcgttccggc ctcgatccac ggagtaggat gcccggcgat
     8881 agccgccctt accctcacgc cgccgtgacc ggcctccctc agtgccccgt agacggtgcc
     8941 gcaggcgcgg gtcggggagt tgttggtctt ggagaggtgg gcgagcacaa ggtctttgag
     9001 gccgtacgga gccagcgcga cggccgcctc acccgtctgc cggttggaga ggtggccgtg
     9061 gcgggaggcg atgcgacgct tcaggctcga tgagtagggg ccgcgcctga gccagtcgtg
     9121 gtcgtggttc gcctcgagga ccaccgcgtc ggcgccgcgc atgcaccgca gcacgtcgat
     9181 gccggcctcg ccgaggtccg tggccatcgc caccgtgctc gcctcgtccg agatcctgac
     9241 cccgtaggtc agggaatcgt gcgggaccgg gaagaacgtc accgtgagcc cgccgacgac
     9301 gcacggctcc tccggggaga ccgcgctggc gccgagcgac tccccaacgc cgggggcggc
     9361 gtagaccggc acgggacggc cacgaagtat ggagcggaca ccgcacgtgt ggtcggagtg
     9421 cccatgggtg aggaggatcg cccgcacgtc accaaggttc ctgccgatgc ccccaaggag
     9481 cgattcgatg cggcgccgcg agagcccggc gtcgaccagg aggccgcccg tgtccgtctc
     9541 catgtaagtg gcattgccag agcttccgct cgaaagcacg ctaaaccgca agtctgccct
     9601 caacctagat cggggtcgat tttcttcccg aaagtgatct tagcacgggg gcgtcaagtg
     9661 ctctcttcga ccagctctcc ctgaacgatg aggcgttgct cgtaatctgg aagggtgcag
     9721 gtctggagcg agacgataga tttgccgtcc tggggttcca tgacctcgac gttgtcaggg
     9781 tccacgattt tctgtccggt cacgcggtac ttgtaggtgt tcccggcctc atcctcgagc
     9841 ttgatctcat ccccttcccc cagctggtcg aggtagtaga agatgaggta ggatcccgtg
     9901 ccctcgtacc cgagacggtg gccggcgatg taggtgttcg cgccatcttg ccaggggaaa
     9961 ccggttgctg gtacgtgcac gacggactcc tcgagcttct catcctctac cgagttgtag
    10021 acgggcacgt cggtgagccc taggtcagga acggtgagat agagggtctt gtcgtcgggc
    10081 ccgcccgggt ccctcccgag gttgaatggt ttcacggccg gtatcgtccc ggcagaggta
    10141 tccgaggtct ccacctctat cgagtcggaa ccctcccggt gccaatcctc gtacttcggc
    10201 ggggacttct cgaggtcctt ctcgtcgagc acatcttcgg ggagaactgc ctctgtgctc
    10261 gtctgggagt cctgctcctc tgagggctcg gcggcctgat cttcggcatc gcctccggcc
    10321 tcgaggtcta ttcccgacac ggccacgttg aaggccgcac ctttcggcat cgccgagccg
    10381 tctgtgttgt agatctccca ccgctggctg ccgctgtcgt acctgacgcc gacggggtgg
    10441 tcgttgtagg tgccgctacc acccccgggg ttccagttct gggtgaccga gaggatcgcc
    10501 tccggtttgc cgttgacgag gggatcgtcc agtgaggtgc tgttatcttc ggtattgtcc
    10561 ggcccggccc ggtggacgag cttctccgcc cctcccggga ttaccacctg aaaggtcgtc
    10621 ccatccggca ccgccgcccg gtcctgattg aagacggccc accgctgggc tcgaggctcg
    10681 taccagacgc caacgttgtg gtcgtaggta ccctcggtat cgttaccctg ctctggtaac
    10741 gaacttacga gaacgacggc attcgggtcc ccgtcgatgc tcgggtcgga gaggtaggtg
    10801 taatcgccgc ggctgttttc ctcgtcgctg cggtggacga atgcggtgtc catcgcctgt
    10861 tctgaaccct gcgccgcagc gttgtggtcc tcggccgcac gcgtgacctc cgctttatcg
    10921 atgctggtcg tctctaccgt cttgccggtc gatccttccg gactcctatc acccgccgtg
    10981 aggctgcagg cggataacga aaagaggacg cacaggagga gcggggcgag agttgccccg
    11041 ctcctcctga atagacgcgg gttcacggcg tgatgccttc gtggaccagg tcggggttag
    11101 acgtgagcca ggcggtacat ttcctcgggc gagagcttcg ggccgcccgt gctcagcatc
    11161 ttgccgccca taaacggtgc gacggccgcc gagacctcgt cgagagtaag tgccttgggg
    11221 aaggcctcgt tggcgtaggg aaccacaccg agcagccagt tgatggccac cacgtgctcg
    11281 ccctcgacgc cggcgatgct gccggccgcc gccaggatat cggggctgga gatcaggggc
    11341 gcggccccca ggtaagcacc gacgcccacg ggctctagcg tggccgccgt ctgatgtatg
    11401 gcggcctcgc tagcggtcgc gtccgccggg aaagtgaagg tcggcttctc gaccggcgtc
    11461 gcaccgacgc cctccagaag ccccactagc gcgtcgacgt gctgtgactc gtggctctcc
    11521 aggttctgta cgacgccaag ggcatcgccg gagagggtgc ctgagtcgag cgccagggcg
    11581 tagaaatccg cctccaggta ctcaagcgta agggcgaagt tcgcgatgtc cacgtcgtcg
    11641 gttttcgcgg aggcgcgccc cgcaaacagc gtcgaggcgg cggtcgcgcc cgcgccagct
    11701 acagctaggg ccttgaagaa gtccctcctg gtcctcggcc gcgcgagctc gtgcgcgcct
    11761 ggaacgttga tcttatccat gttctctctt tctcctccct aaccggtgat gccgaacggt
    11821 tcgacggccg cctgcacctt ctgcaggggc aacgccacct cgaaggcgag gtcgttcggc
    11881 gggttgatga ggttcaggat gttgatcgcg caccggtgct ggcactcggc gttgtgaatc
    11941 gagatggccg cggcaaggat gtccttgctc ttcagcgccg gggccgcgcc ctggtaggcg
    12001 ccgatgccca cgggctcgaa ggtggccgcc agctccagaa acgttgccgc gtccgagaag
    12061 gtgccgctcg gaaaggtgaa ctcgggggta gccggcacct cggcccccag ctggccaagc
    12121 gttgtggcca gggcgtcggc gtgcgccatc tcgtggtccc tgatggcaac tatctgctcc
    12181 aggaacgcgt ccttgaatat gccggcattg acgccttcgg cgtagaacgt gccctcgagg
    12241 agctctagct ggtaagcgaa cgctactatc gccgcgtcgc ccaggccctc gtaagtctcg
    12301 tactcaccct ccgcctcggc cctgcggacg gcgacgccga agccgccgat ccccatagcg
    12361 gaaagcgcag cgcccgtcag ggccgccgtc ttcaggaagt tccgccgcga ggtctcccca
    12421 tccatcccga tggggacgcg gctctctact ccactcatct gtacgtgcct cccttttcct
    12481 ggcccactgc gagaccggtt gccggtacct tccaccctgc cttacggaag gcggcggacc
    12541 gcggatcaca acctaccaac cacagacgga ccaggttcgg ggcactaccc cgcgtttcag
    12601 gttgctctcg aagaggagtt gatccaggac cgtgtcgttg ccgtagaatc aggttatggc
    12661 ggaccgggga aaacagtact tgatcctggc cgacgaggac ctgatctccc tcctcgaggc
    12721 ttctgactcc gaggcatttg ccacgctcta cgaccgccac agccgcgccg ccttctccct
    12781 cgcctacagg atgatgggcg accgtcaggc ggccgaggat ctcgcccagg acgcctttct
    12841 aaaggtctgg cgtggggctg gcagctaccg cgccgagcgg ggcagcgtca ggacctggat
    12901 cctctctatc gttcacaaca ggggcataga ccagctgcgc tcccacgcga gccggcgcag
    12961 gacgcaggac aagatagagg cctcggcccc ccgctcccag ccgagcgagg ccttcgccga
    13021 aacctggcgc aactcgcagc gcgagcaggt gcgagaggcg ttgaacacgc ttccgagaga
    13081 gcagctgaag atactggagc tcgcgtactt ttcgggttat acccacgcgg agatatccga
    13141 gctcctcagc ctacctcttg gtacggtaaa gggcagaatg cgcctggggc tgaagaagat
    13201 cagggacttt ttcgagtcga gaggcgcggc ggtaccgaga tgaacgagat gaacaacgaa
    13261 cgcttcgaag atctgaagga cgcttacgtc ctcgacgccc tcgccgagga ggagaagcgc
    13321 agcttcgagg aattcctggc ggcccacccc gagcgccagg ccgagataga cgagctcggc
    13381 gcggtcgccg ggctcctggc cttctcgcca gaagtgcagg agccgccccc agacctccgc
    13441 accaggatca tgaacgtggt cgccgcagag gcggctcccc gacgggagag acagggatcg
    13501 gtgttgggcg gtctctgggg ctacctgggc gccaggggcc tcgccctcgg tgccgcggcg
    13561 ctgctcctta tcggcctgct ctcgtggaac ctgttgttgc aggaccaggt agacgacctg
    13621 cagggtcagg tccagagttc gcagggtcag gtcgaggacc tgcaagccca ggttcgggag
    13681 gcccaggccc agcaaaccca gaccgtccag ctcagcggaa cctggacaac ccagggggct
    13741 gacgccgagg tagcctcgat tagcggcgac aggatcatcc tggtcgccga cgacctgccc
    13801 gccatcccgg aaggtcaaac gtgccagatc tgggtgatca agggcgatgt tccagagcca
    13861 agtggcctct tccagccggg cggaacggag actgccgcgc cgattacgac ccccatcaag
    13921 aagggcgaca cgatagccgt gacggtcgag cccgccggag gctcggagca accaacgacc
    13981 gacccggtgc tgtccgccga gctgtaagta gacgcattcc agaccggtcg acgtaccgcc
    14041 ccgccaggca cgacccgaga cccgaaacgt cttttttgtg caatcctcag ccacgttggg
    14101 aggttggaca cagcgggttc tgaggaaacg ctgtataccc gaaccgtgat acgttttggt
    14161 tatcctgcgc agaacctgac gcttccggcg accaccaacc gcacgctgcg tctggcaaac
    14221 atcccccacg tggagaagct tcgggggctc gtccggggaa acctggccga ccttagatcc
    14281 attctgagat ggaacgcgga gcacgggatc gcgctcttca ggatggggca ggatcttatc
    14341 cccttcgcct cgcatccagc gtttccttac gactggcagg acgagcacgg cgaagagttg
    14401 cgcagcatcg gagccctgtc gcgggacctc ggcatccggc tatccatgca tccgggccag
    14461 tacatcaacc cgggcagccc gcggccagag gtggtagagc gcagcttgtg ggagctcgaa
    14521 tacgtggcct ccgttttcga cctgctcggt aaccgcgaca gcgtggccgt tctgcacatg
    14581 ggaggcgcct acgcggacag ggcagggagc gccgcgcgtt tcgtggaggc gctgcgcggt
    14641 gagtcgcgta tcctgcgcta cctcgccctg gaaaacgatg agcgcgtctg gaccgtagcc
    14701 gaggtagtcg aggcctcccg cctcctcggc atcccgacga tcatggacgc gttccaccac
    14761 ggcctgaacc ccggcgacct cacgctggag ggagccctgg acgcggcgct gcccacctgg
    14821 gagatcaggg gcgttcgtcc caagctgcac ctctccagtc aggacccgga caaacggccg
    14881 ggcgcgcacg cccccactgt agaagagaga gactggcagg cactgctcgc ggcgctgggt
    14941 ggcagggacg ccgacgtgat ggtcgaggcc aagagcaagg agcgtgccct ggcttccctg
    15001 ggcgtgaaga tggggtaaca tcgtgtcatc tcgaagtagg gtaggagcta tagacgtgca
    15061 caagaacccc gacaggcaag agatcagggc ccttctggac cggtcgcgga ccgtggccgt
    15121 ggtcggactt tcggatagcc cgtaccgtac gagccacgcg atcgccgggg cgttgcaggg
    15181 gttcggatac aggatctttc ccgtcaaccc gaacctttcc ggcccagtcc tcggggagct
    15241 tccctacgac cgcgtagtgg atgtccccac accggtcgac gtggtcgacg tgttccggcg
    15301 cagcgagaag gtgatgcccg tggccgaaga cgccgtggcc gcgggcgcga aggtgctgtg
    15361 gctacaatcc ggcgttgtca acgaggaggc tgcggcctac gccacagagc aaggcctgac
    15421 ggtgatcatg gaccgctgca tcaaggttga ttacgcgtcg ctgataggcc gatgagccgg
    15481 ccagcgctcc aaccgccgac aggtcacagg cccctctgag gcggacggcg atgctcgcgg
    15541 gctgacatgc tgaccatatt cgtctacggc acgttgaagc gtggcgagcg caaccacggc
    15601 tactgtagcg gtgctctgcg cgtggaagaa ggcgcggtgc gcggcgagct gtacgatctt
    15661 ccccgcttcg gctacccgga gctgatcgtt cccgaagagt ccgtgcgcgc cttcggtacg
    15721 gatgattacc tcggggacgc cgagctgcaa gcacgtctct ccccgggaag tccatccccc
    15781 ccggaaagtc ccctggtcta cggggagatc ttggttttcg atgacccggg atcccgcctc
    15841 ccggctatcg accggctgga ggggttcgac ccagccgaag actcaagccg ttaccggcgc
    15901 gtgttgctgc ccgtcgagct ggaggagggt ccgaggctcc tggcgtgggc ctacgcggta
    15961 agcgagccct ggggagcgca cgtcccagac ggaaggtggc caccctaggc gaggctcgcc
    16021 gttgtattga agggttatga accctcctct ataccctagg ggttggaggt gggggcgtcc
    16081 tgatcctcct cgattatctc ttcgcgtatt acgcggcggc ggatcttggt gcgaccgccc
    16141 tcgcggaacg tctcggtctc ttcggtctcg cggatggtcg gcgtgctctg gggagcttcg
    16201 tcggtgaagg gacccgtact ttctgggctc gtgcctgccg agagggagtc ggcccccggg
    16261 gattcactcg tctccccgaa cccacctggt ctctccgagg ttcctgcggt cggtaccgtg
//
LOCUS       DBNGVD010000058         8184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3106208, whole genome shotgun sequence.
ACCESSION   DBNGVD010000058 DBNGVD010000000
VERSION     DBNGVD010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3106208"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="AC16CM_01620"
     CDS             complement(<1..1507)
                     /locus_tag="AC16CM_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR3138554.1"
                     /translation="MLEGLAQDFQLTLPYILRRAEQLYGYREIVTRRPDKSFHRYTYS
                     DFVSRSKKLAVALGELGLEPGDRVGTLGWNTYQHLEAYFGIPSAGLVLHTLNPRLHED
                     DLAYIADHANDKVMLVDENLLPVFEKFRGKTEIEHAVLVTENGDVPDGMISYEDLLSG
                     ADEDAFEYPDFDERQAAALCYTSGTTGRPKGALYSHRSTVLHSMMASMGNTFALSEED
                     CLLPVVPMFHVNAWGLPFASVLVGAKLVMPGPHLDPASLLEELEAEEVTLTAGVPTIF
                     LALLQALDEEPERYDLSRLRSMVIGGAAVPKGVIERYQERYGIDVVHAWGMTEMSPIG
                     SVANLTSEMRKLPKDEQLEYKAKQGYPVPLVEIRARGDEGLVPWDGETPGELEVRGPW
                     VARAYFEAPEGDDKFTDDGWFKTGDIVTIDRFGFIKITDRSKDLIKSGGEWISSVELE
                     NALMSHPSVSQAAVIAIPHEKWDERPLAAIVLMEGETVTEEELHSHLEADFA"
     gene            1642..1839
                     /locus_tag="AC16CM_01625"
     CDS             1642..1839
                     /locus_tag="AC16CM_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138555.1"
                     /translation="MPSSVEGQNFEVVAVTLFGKMVVASYPTLEQAEWRAGQLAAEAE
                     RSPRGYLQYLVRPAGEASGKP"
     gene            1917..2789
                     /locus_tag="AC16CM_01630"
     CDS             1917..2789
                     /locus_tag="AC16CM_01630"
                     /EC_number="3.4.-.-"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3138556.1"
                     /translation="MGPGKHAARVIFAEYWRRHRLKLAAVALILVGLIAFWLSPVGPE
                     ELTLASSVRLSTEGSPQPPEDEAGTVPVWARKNEAQRHVMLENARQGASQEGDTCDNR
                     LVLVDRPHALPQGYVPNDLVSLQAAGVPTVGGRNLMLRREAAEHLKDLTAAATADGEE
                     LVVASAFRSYLHQRYTYERLKSIYGSEADTMSASPGHSQHQLGTAVDFTNWVAAYQVQ
                     PIFGHTSAAWWLRDHAPTYGFVLSYPPGKNETGYRFEPWHYRYIGVENAGRLQKSGLS
                     LQEFLVREGVSPRC"
     gene            2903..3025
                     /locus_tag="AC16CM_01635"
     CDS             2903..3025
                     /locus_tag="AC16CM_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138557.1"
                     /translation="MSTEYVQVAGALVFFLAILIGSYWFVYVPNREGQKRKDEG"
     gene            3064..3528
                     /locus_tag="AC16CM_01640"
     CDS             3064..3528
                     /locus_tag="AC16CM_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3138558.1"
                     /translation="MQVSAKTDYALRAASELALAATDGAGPIKGDRISETQGIPKKFM
                     ENILHDLKRSGLVRTRRGASGGYWLALPAEEISLADIIRAVEGPLANVRGEWPEVVEY
                     SGAAENLQQVWIAVRANLRAVLESVTLADIVEGELPPSITELTDDPEAWVHH"
     gene            3888..4667
                     /locus_tag="AC16CM_01645"
     CDS             3888..4667
                     /locus_tag="AC16CM_01645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138559.1"
                     /translation="MYQNTVFDAGCEVRLWEEDGELVGFAWLEKPDGVVMQVHPRLRG
                     SGPLEREMLEWGARQTRAVYGEQQEDELWTRVTEDEPRLAALLAELSFEPDPDHALMM
                     SRDLHAPIPGSILPDGWTVREVGGEEEWEARVEIHREVWHPSRVTLEAYRRLRAAPLY
                     NGRLDLVAAAPDGSFGSYCICWYDPGSHTGLFEPVGTRATYRGLGLGKSVMHEGLRRL
                     RDLGAHTASVVAFHDNEAARNLYQSVGFETVNTERLYGKKL"
     gene            4752..6086
                     /gene="chrA"
                     /locus_tag="AC16CM_01650"
     CDS             4752..6086
                     /gene="chrA"
                     /locus_tag="AC16CM_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3138560.1"
                     /translation="MKNGKVAGVGTKSVRFWEAFWFWVKLGFINFGGPAGQIAIMHRE
                     LVEKKRWVSEKQYLRTLNFCMLLPGPEAQQVATYIGWRLHGTLGGVVAGSFFVIPSIF
                     VLWLLSYLAVAHSDVPAITGLLYGIQPVVIAIVVEAVLRIGKRTINNRVLVGFAVLAF
                     VALYFLSVPFPLVVLVAAVAGLLLSRTSLAADAFGVGGEGSLEEVEEESAIDRTGSHG
                     MPSMLRNVRLLGTFVVLWALPLGALYLWRGGEDVLVKEALFFTGAAFLTFGGAYAVLS
                     YVADVAVNHYGWLTADQMVQGLGLAESTPGPLIMVTEYVGFIGAWNDPGPYAPLLYGT
                     LGALVTVYVTFLPCFLFIFFLAPYIELLANDTRLKAILVGVTAAVVGVIANLAVYFAT
                     QVLFPDGVSLQGLDVFALVVAAVSFVVLQRFRVPIYLMVPAGALVGMAWTLL"
     gene            6071..6589
                     /locus_tag="AC16CM_01655"
     CDS             6071..6589
                     /locus_tag="AC16CM_01655"
                     /inference="COORDINATES: protein motif:HMM:NF042524.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HIR3138561.1"
                     /translation="MDSSVGGKVRWLILIYHLPREPSRHRVAVWRKLKALGALYLQDG
                     AAALPEDAVTREQLEWLQVRVREAGGEATLWEALPNTVAENAALVKEFRALREAAYRE
                     LIDSAGRLRQKAAMGAGRTVLLGELARIERDFRSERRRDYFRSPLRKVADAAIREVRS
                     IVKNRPVREDVG"
     gene            6589..7005
                     /locus_tag="AC16CM_01660"
     CDS             6589..7005
                     /locus_tag="AC16CM_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR3138562.1"
                     /translation="MLWATRRGCHVDRTACIWLIRRRIDPDAEFSFFVDASEAPEGAE
                     LFDVAGARLSHRGDACSFETMLEEYDLDDTVLREISEIVHDADLMDDMYGRSEADGLD
                     AVIRGMQLALPDDNVLSVHTDIIFDGLYAYLWRETL"
     gene            7007..7717
                     /locus_tag="AC16CM_01665"
     CDS             7007..7717
                     /locus_tag="AC16CM_01665"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138563.1"
                     /translation="MAPTGIEVRGLGYELHGAEILKDVDVLVREGETTAVVGPSGAGK
                     STLLRAINRLIEPTSGEVYMGGEPTSRLDPLELRRRVGMVFQVPALFGGSVAEAVLYG
                     ARLAGKDADPGRLLEMVGLSASLAERDPQTLSVGQQQRVAIARALALEPEALLLDEPT
                     SALDEGARRRIEELVTDLNARLGLTMVFVSHDLSQVERVADRVVVLAEGKSMGEWDRD
                     DFFSRAGERARRYIEGRL"
     gene            7719..>8184
                     /locus_tag="AC16CM_01670"
     CDS             7719..>8184
                     /locus_tag="AC16CM_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138564.1"
                     /translation="MVGLQTVGSALLTLGLVAIAVALSLYERLDLEKDIGVAVVRSFV
                     QLAAIGYVIDYVFGLENLWVVVMLLAGMVLFAAWTSARRAREVPNAFPIAVVAVGVAA
                     VATLVLLLALGIVPATARYLIPLGGMVIGNSMNASSLTMTRIRDDLAEQRLKV"
BASE COUNT         1374 a         2513 c         2767 g         1530 t
ORIGIN      
        1 tggcgaagtc ggcttccagg tgggagtgca actcctcctc ggtcacggtc tcgccctcca
       61 tcaggacgat ggctgccagg ggccgttcat cccatttctc gtgggggatg gctatgactg
      121 ccgcctggga gacggacggg tgggacataa gggcgttctc gagctcgacc gaggagatcc
      181 actccccccc gctctttatg aggtccttgc tccggtcggt gatcttgatg aacccgaagc
      241 ggtctatggt gacgatgtcc ccagtcttga accagccgtc gtcggtgaac ttgtcgtcgc
      301 cctccggggc ctcgaagtat gccctggcga cccaggggcc ccttacctcc agctcgccgg
      361 gcgtttcgcc gtcccagggc accagcccct cgtcgcccct ggcacgtatc tccaccaggg
      421 gcaccgggta gccctgcttg gccttgtact ccagctgctc atccttgggg agcttcctca
      481 tctccgaggt caggttggcc acgctcccta tggggctcat ctccgtcatg ccccaggcgt
      541 ggaccacgtc tatcccgtac cgctcctgat acctctctat tacgcccttg ggcaccgcgg
      601 cccccccgat aaccatggac cgcagcctgg agagatcgta ccgctcgggc tcctcgtcga
      661 gcgcctgcag cagggccagg aagatcgtgg ggacgccggc ggtgagcgtg acctcttctg
      721 cctccagctc ctcgagcagg ctcgccggat cgaggtgggg ccccggcatc accagcttgg
      781 ctccgacgag aacggaggcg aatggcagac cccaggcgtt gacgtggaac atggggacga
      841 cgggtagcag acagtcctct tcggagaggg cgaacgtgtt gcccatcgag gccatcatgg
      901 agtggagcac ggtggagcgg tgtgagtaga gggccccttt tggccggccc gtggtccccg
      961 aggtgtagca cagcgccgct gcctgcctct cgtcgaagtc agggtactcg aaggcatcct
     1021 cgtcggcgcc ggagagaagg tcctcgtagg agatcatgcc gtccggcaca tcaccgttct
     1081 cggtaaccaa cacggcgtgt tcgatctccg tcttgcccct gaacttctcg aagacgggca
     1141 ggaggttctc gtcgaccagc atgactttgt cgttcgcgtg gtctgctatg taggcgaggt
     1201 cgtcctcgtg caggcgggga ttcagggtgt gcagcacgag gcctgcggag gggatgccaa
     1261 agtacgcttc caggtgctgg taggtgttcc agccaagagt gcccaccctg tcgcctggtt
     1321 ccaacccgag ctcgccgagg gcgacggcaa gcttcttgga gcgggagacg aagtcggagt
     1381 aggtgtagcg gtggaaagac ttgtcgggac ggcgggtaac tatctcgcgg tacccgtaga
     1441 gttgctccgc ccgccgcagg atgtagggaa gggtgagttg gaaatcctgg gcaagtccct
     1501 ctagcatatc ttctcctctt tctgtggggt gcagggcgcg ccacatggcg cgcccccggc
     1561 gcctgtactc cctctcccgg aaagcattgt tgggagcgat cttagcaggc acaatgaaag
     1621 ggggtcaaga taggagtaac ggtgccgtct tccgtagagg ggcagaattt cgaggtcgtt
     1681 gcggtaacgc tcttcgggaa gatggtggtc gccagctacc cgacgctgga gcaggccgag
     1741 tggcgcgccg gacagcttgc cgccgaggcg gagcgcagcc cacgaggcta tctacagtac
     1801 ctggtgcgcc ccgccgggga agcctccggc aaaccctagg gcgacccgcg tggaggaccc
     1861 ctgggagaac tccctgggcc tggacagaac cgcgacgata cgttagcttg cgtggcgtgg
     1921 gaccaggtaa gcatgctgcc agggtcatct tcgccgaata ctggagacgg caccgcctca
     1981 agctcgccgc ggttgctttg atccttgtcg gcctcatcgc cttctggctc tcgcccgtgg
     2041 ggcctgaaga gctcaccctc gcatcctccg tgcggctctc cacggaaggg tcgccccaac
     2101 ccccggaaga cgaggcgggg accgtccccg tctgggcccg caagaacgag gcccagcggc
     2161 acgtgatgct agagaatgcg cggcagggcg cgtcgcagga gggggacacc tgcgacaatc
     2221 ggctggttct cgtcgaccgc ccgcacgcgc tgccccaggg ttacgtcccg aacgacctcg
     2281 tctcgctgca ggccgccggc gttcccacgg tgggcggccg aaacctgatg ctccggcgcg
     2341 aggcggcgga acacctgaaa gacctcaccg ccgccgcgac cgcggacggt gaggagctcg
     2401 tggttgcctc cgccttcaga tcctacctgc accagaggta cacctacgag aggttgaaga
     2461 gcatctacgg ttcggaggcc gatacgatga gcgcctcgcc ggggcacagc cagcaccagc
     2521 tcggcaccgc cgtggacttc accaactggg tggcggccta ccaggtgcag cccatcttcg
     2581 gccacacctc agccgcgtgg tggctacggg accacgcccc cacgtacggc ttcgtcctct
     2641 cctacccacc cggcaagaac gagaccggct acagattcga gccctggcat taccgctaca
     2701 taggcgtcga gaatgccgga cgtctccaga agagcggcct ctccctgcag gagttcctgg
     2761 tacgcgaggg cgtatcgccc cgttgctgag gcccgcgacg gagccggtga cggagccggt
     2821 gacggagccc acggcgaagc cggcgctact gtcgtagaat tgtggtgtca gacgtctgag
     2881 ggttgcgtga ttaggaggtt cgatgtcgac ggagtacgtg caggtcgccg gggcgctcgt
     2941 gttctttctg gcgatactga tcgggtctta ctggtttgtc tacgtgccga accgtgaagg
     3001 acagaagaga aaagatgagg gttgatgcgg gcgcaggtcg tctcgtgggg agcgtgaact
     3061 agcttgcagg tttccgccaa gaccgactat gcgctgaggg ccgcctccga gctggcgctc
     3121 gccgccaccg acggggccgg ccccataaag ggagacagga tctccgagac ccagggcatc
     3181 cccaagaagt tcatggagaa cattcttcac gatctgaaac gctccggcct cgtgcgcacc
     3241 aggcgcggtg cctctggcgg ctactggctc gcgcttcccg cggaggagat ctcgctggcc
     3301 gacatcatcc gcgccgtgga ggggccgctg gccaacgtga ggggcgagtg gcccgaggtg
     3361 gtcgagtact cgggcgccgc cgagaacctg caacaggtct ggatcgcggt ccgcgccaac
     3421 ctgagggccg tgctcgagtc ggtgaccctc gccgatatcg tggaagggga gcttccgccc
     3481 tcgatcacgg agctgaccga cgatccggaa gcatgggtgc accactagca cgcgcccttc
     3541 ggtcgcttgt cagcacgtta cggtccttcg gacctccact ttcggcacgc ctgcttcgca
     3601 ggcttgtcgg tcacaccgca aacctctggc attgaagtgc attcggacct ttggctttgg
     3661 caggttcccc gcattcgtca ttcccgcgaa agcgggaatc cacgggtagt cgaagaagga
     3721 ccgtggaaga gaacggggct cgaccctgat atgcaccagg gcccactgtg aaatcgcgtc
     3781 cttttggtga ggaggcagac cttcgatcga tcatcgaatt tctgtctcgg actacttctg
     3841 ccgggctggc gggggcactc tggcatcccg gtgatgcggt gtgggggatg taccagaaca
     3901 cggtgttcga tgccggctgt gaggtccggt tgtgggaaga ggatggcgag ctcgttggct
     3961 ttgcctggct ggagaaacct gacggggtgg tgatgcaggt gcatccccgc ctgcgtgggt
     4021 ccggcccgct ggagagggag atgctcgaat ggggtgctcg tcagacccgg gccgtttacg
     4081 gcgagcagca agaggacgaa ctgtggacgc gggtcaccga ggatgaaccg cgcctcgccg
     4141 cgctcctcgc cgaactcagc ttcgagccgg accccgacca cgcgctgatg atgtcacgag
     4201 acctccatgc cccgatcccc gggagcatcc taccggacgg ctggaccgtg cgtgaggtcg
     4261 gtggggaaga ggaatgggaa gcgcgtgtgg agattcatcg cgaggtctgg cacccctcga
     4321 gggtcacact ggaggcatac cggcgcctga gggccgcgcc gctatacaac gggcgtctcg
     4381 acctcgtcgc cgcggccccc gacggttcgt tcggctccta ctgcatctgc tggtatgacc
     4441 cggggagcca caccggcctc ttcgagcccg tcggcacgag ggcaacgtac aggggactgg
     4501 gcctcggaaa atccgtcatg cacgaggggc tgcgcaggct tagagacctc ggcgcccaca
     4561 ccgcctccgt cgtcgcgttc cacgacaacg aggcagccag aaacctctac cagtccgtcg
     4621 gcttcgaaac ggttaatacg gaacgcctct acggcaagaa actctagaac aaacctccga
     4681 ttcctctcct tgacttccga tgtaaccatg gttactctac acctgttgtt cactaagaaa
     4741 gtagagataa gttgaagaac gggaaggtgg caggagtggg gaccaaatcg gtcaggttct
     4801 gggaggcttt ctggttctgg gtgaagcttg gcttcataaa cttcggcggt cccgccgggc
     4861 agatcgccat catgcaccgt gagctggtcg agaagaagcg ctgggtctcg gagaagcagt
     4921 acctgaggac gctcaacttc tgcatgctct tgccagggcc ggaggcgcag caggtcgcga
     4981 cctacatcgg gtggaggttg cacggaacgc tgggtggtgt cgtggccggc tccttcttcg
     5041 tcatcccctc catcttcgtc ttgtggctac tctcgtacct ggccgtggcc cactccgacg
     5101 tgccggcgat cacgggtttg ctgtacggca tccagccggt ggtgatagcc atcgtcgtgg
     5161 aggcggtctt gaggatcggc aagcgcacga tcaacaacag ggtgctggtc ggcttcgcgg
     5221 tcctggcatt tgtcgccctc tacttcctct ccgtgccctt cccgctggtc gtcctggtcg
     5281 cggccgtggc tggcctgctc ctgagccgca cgagcctcgc cgcggacgcg ttcggggtag
     5341 ggggagaagg ctctctggag gaggtagaag aggagtcagc catagaccgt accggctcgc
     5401 atggcatgcc ttccatgctg cgtaacgtga ggctgcttgg gaccttcgtg gtgctctggg
     5461 cgctcccact tggtgctctg tacctgtggc gtgggggaga ggacgtgctc gtaaaggagg
     5521 cgctgttctt cacgggagcc gccttcctga ccttcggggg ggcttatgcg gtactgagct
     5581 acgtggccga tgtggcggtc aaccactacg ggtggctcac cgccgaccag atggtgcagg
     5641 ggctggggct ggccgagtcc acgccgggtc cgctaatcat ggtcaccgag tacgtgggct
     5701 tcatcggtgc ctggaacgat ccggggccgt acgcgcccct gctatacggg acgctcgggg
     5761 ccctcgtcac cgtctacgtc acctttctgc cttgcttcct gttcatcttc ttcctggcac
     5821 cttacataga gctactcgcc aacgacacga ggctcaaggc catcctcgtg ggggtcacgg
     5881 cggccgtggt cggtgtgatc gccaacctcg ccgtgtactt tgccacccag gttctgttcc
     5941 ccgacggcgt gtccttgcag gggctcgacg tattcgccct cgtcgtcgcg gccgtctcct
     6001 tcgtcgtgct gcagcggttc agggttccca tatacttgat ggtgcccgca ggcgcgctag
     6061 tggggatggc atggactctt ctgtaggtgg caaggtcagg tggctcatcc tgatctacca
     6121 cctgccacgc gaaccttccc ggcacagggt cgccgtgtgg cgcaagctga aggctctcgg
     6181 agccctctac ctgcaggacg gggccgccgc gctgcctgag gatgccgtta cccgcgagca
     6241 actcgagtgg ctgcaggtcc gcgtgcggga ggcgggtggg gaggcgacgc tctgggaggc
     6301 cctgccaaac accgtcgccg aaaacgcggc gctcgtgaaa gaattccgcg ccttgcgcga
     6361 ggcggcatac agggagctca tcgactcggc gggcaggctc aggcagaagg ccgcgatggg
     6421 ggcaggcagg acggttcttc tcggtgagct cgcgaggatc gaacgagact tcaggagcga
     6481 gcgccggcgc gactacttca ggtcgccatt gcggaaggtg gccgacgcgg cgatccgtga
     6541 ggtacgaagc atcgtgaaga accgacccgt gcgggaggat gtagggtagt gctctgggcg
     6601 accaggcgag gatgccacgt ggaccggacg gcctgcatat ggctgatccg gcgccgcatc
     6661 gatcccgacg ccgagtttag cttctttgtt gacgcctccg aggcgcccga aggtgccgag
     6721 ctcttcgacg tggccggggc caggctctcc caccgtggcg atgcgtgctc cttcgagacc
     6781 atgctcgaag agtatgatct tgacgatacc gtgctgcgcg agatctccga gatagtccac
     6841 gacgccgacc tgatggacga catgtacggg cgctccgagg ccgacgggct ggacgccgtg
     6901 atcaggggca tgcagcttgc ccttcccgat gataacgtcc tctcggtcca cacagacatc
     6961 atcttcgatg gcctgtacgc gtacctgtgg agggagacgc tctagcttgg cccctactgg
     7021 aatcgaggtt cgcgggctcg ggtacgagct tcacggagcc gagatcttga aggacgttga
     7081 cgtcctggta cgcgaggggg agactacggc ggtcgttggt ccttcggggg cgggtaagag
     7141 caccctgctg cgcgcaatca accgactcat cgagcctacc tccggcgagg tctacatggg
     7201 cggggagccg acgagcaggc tagatccgct ggagttgcgg cggcgggtcg ggatggtttt
     7261 ccaggttccg gccctcttcg ggggctcggt ggcggaggcg gtcctgtacg gggcacgcct
     7321 ggcgggcaag gacgccgacc ctgggcggtt gctggagatg gtggggttga gcgcctcgct
     7381 tgcggaacga gatccgcaga cgctctcggt cgggcagcag cagcgcgtgg ccatagcccg
     7441 cgcgctcgcc ctcgagcccg aggcgctcct gctcgacgag ccgacgagtg ctctggacga
     7501 gggtgcgcgg cgcaggatag aggagctcgt gacggatctg aacgccaggc tcgggctcac
     7561 catggtcttc gtgagccacg acctctccca ggtcgaaagg gtagccgacc gggtggtcgt
     7621 gctggccgag ggcaagagca tgggggagtg ggacagggac gacttcttct cccgggccgg
     7681 cgagcgggca cgacggtata tagaggggag gctctagcat ggtggggttg cagacggtgg
     7741 gatcggcgct tctgaccctg ggcctcgtgg ccatcgcggt agccctgagc ctgtacgaga
     7801 ggctcgacct ggagaaggat atcggggtag ccgtggttcg ctccttcgtt cagctcgccg
     7861 cgatagggta cgtcatagat tatgtcttcg gactcgagaa cctgtgggtg gtggtaatgc
     7921 tcctcgccgg catggtcctc ttcgccgcct ggacctcggc ccgaagggca agggaggtgc
     7981 cgaacgcctt tccaatcgcc gtcgtcgccg ttggggtggc cgccgtggcc acgctggtgc
     8041 tgttgctcgc cctcgggata gtgccggcca cggcgcgtta cctgataccg ctcggcggca
     8101 tggtgatagg aaactccatg aacgcctcta gcctgaccat gacccgcatc cgcgacgacc
     8161 tggccgagca gaggctaaag gtcg
//
LOCUS       DBNGVD010000059         5641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1649469, whole genome shotgun sequence.
ACCESSION   DBNGVD010000059 DBNGVD010000000
VERSION     DBNGVD010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1649469"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC16CM_01675"
     CDS             <1..204
                     /locus_tag="AC16CM_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HIR3138565.1"
                     /translation="AAVIRLRSTASRILALDMLTLILVALLVLYADAQRSPYYLDAAL
                     ILSLLAFLATLALSRYHGERRLF"
     gene            204..548
                     /gene="mnhG"
                     /locus_tag="AC16CM_01680"
     CDS             204..548
                     /gene="mnhG"
                     /locus_tag="AC16CM_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HIR3138566.1"
                     /translation="MAALLPYLADALVVLGVLVMTVGVYGAIRMPDTYTKLHSMSKAV
                     FLGVISFCASSVVTGDPAIICRALLISAFLVITTPISALTIGRAAYLRGERMKNPGAR
                     DESGKGLSRAES"
     gene            complement(690..1604)
                     /locus_tag="AC16CM_01685"
     CDS             complement(690..1604)
                     /locus_tag="AC16CM_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3138567.1"
                     /translation="MDHSSSGPLNRVLVGVDGSEDSALAIRAASDLSNRTGAELHVVH
                     AWQDETPPSLTSAMSGDYSEARQQWERAAEEVLKEQAALAEAAGASVAGTHNRRGRPA
                     EEITDLASELGADLLVIGSRGLGAVKRLVMGSVSEGVVSLSPCPVLVMRGGERAWPPS
                     RVVVGADASEEARRAAELGAGLAGALGVEVALVTAYQNPVPLTGGRTHAPGAGAERAS
                     EEAREALSRLATGLEDTIGVRPERQAVLGDSAAVIQDAAEEDGPALVVVGSRGRGAVT
                     RFALGSVSMNVLRAVEGPVLVVHAADEA"
     gene            1792..2397
                     /locus_tag="AC16CM_01690"
     CDS             1792..2397
                     /locus_tag="AC16CM_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR3138568.1"
                     /translation="MITLALQGAIVVFLILVNGLFSMSETALVSSRKAVLRQRAESGD
                     AGALYALDLAESPNRFLSTVQIGISLIGVLSGALGGATLSRPLAGLLSETFPGLAPYA
                     GVITFGVVVGSITYLTLILGELVPKRLALNGPEKVASRVAGPMRLLSTLAAPAVWFLG
                     VSTDTVLRLLRVRPSDEPPVSEREVEVMLEEGARQSPGKLL"
     gene            complement(2394..2963)
                     /locus_tag="AC16CM_01695"
     CDS             complement(2394..2963)
                     /locus_tag="AC16CM_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138569.1"
                     /translation="MHELMFLQLQKDRHSTTVHEVERERLATAARAWGPAKTPVRMGE
                     AGNVLAAKPFWLLVKVEAGRIYIMTTSLATGGTALPVFSFEEEAAMFRDLGAPHVGWR
                     VEQTVDTGLVSVLQRPCADVDMVLLDPLPGVDAGTSADLVGVGREPFLEFLSRTAGER
                     SRRSRPPHPQESTPHLTPGRQSEMLVVGV"
     gene            complement(3055..3210)
                     /locus_tag="AC16CM_01700"
     CDS             complement(3055..3210)
                     /locus_tag="AC16CM_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138570.1"
                     /translation="MQEVIREEYVRRMATQEEIERILSDPRILEGVREQVRRDLRFEG
                     ASTRRTP"
     gene            complement(3519..4661)
                     /locus_tag="AC16CM_01705"
     CDS             complement(3519..4661)
                     /locus_tag="AC16CM_01705"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3138571.1"
                     /translation="MRADDRPSAGDGVDLNLDTPALVVDAQRLEQNLLGMAAVAESRG
                     VALRPHVKTHKIPSLARRQMEAGAAGITVATISEAEVMADGGLDDIFVAYPLVTEIKI
                     GRAIGLTRRGMRLILGVDGRHGAHRLSSLAGDAGVTLEVRLEVDSGLGRTGVSPAEAA
                     GLAREISSLENLDLTGVYTYRGAVLGDGSPTTDLEAAGRLEGELMASVAQEIRDAGVP
                     VEDVSVGSTPTAGHAAGVDGITEIRPGTYIFYDRMQAALGVCSLSDCALRVLVTVVSR
                     PRPDLAIIDGGSKTFATDVQPKVAPLDLTGFGHVVGHPGAVLERVTEEHGMLSVDADS
                     GLRVGDTVEIVPNHVCSTVNLHDELFMLYADGGTERVPVAARGMVR"
     gene            complement(4689..>5641)
                     /locus_tag="AC16CM_01710"
     CDS             complement(4689..>5641)
                     /locus_tag="AC16CM_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138572.1"
                     /translation="TTSGIAIAYFVGAALAVTESWRLILSLSVIPAVIVLLLVVRLPD
                     TARWLLMNGQREEAVDLLRRVNPDMDSEREADLIEEDLSYEEKGSFAELFQGRFRRAG
                     IFVVGLGFLVQITGINAIVYYSPTIIQQVGVTSPTGAIIATGFVQIAGVIAEVVAFLV
                     VDRWGRRPTLLTGIGTMAFANLLLVFTYGLGLSAAFTLVGILLFTVGFNFGYGSLVWV
                     YASESFPARLRTQGGSAMLTADLFANFIVGVVFLSALGALGGSMTFGIFLLLSVVAIV
                     FVYNLAPETKGRQLEAIRAYWYNGGRWPDESESQTTRTGA"
BASE COUNT          897 a         1984 c         1745 g         1015 t
ORIGIN      
        1 gcggcggtga tccggctgcg ctccacggcg agccggatac tggcgcttga catgctgacc
       61 ctgatactgg tggcgctcct ggtgctctac gcggacgccc agcgctcgcc ctactacctg
      121 gacgccgcgc tcatcctctc gcttctggcc ttcctggcga cgctagccct ctcccgctac
      181 cacggggaga ggaggctctt ctagtggccg ccctcctccc ctatctcgcc gatgccctgg
      241 tggtgctggg cgttctcgtc atgaccgttg gcgtctacgg ggcgatccgg atgcccgaca
      301 cctacaccaa gttgcactcc atgagcaagg ccgtgttcct gggcgtgatc tccttctgcg
      361 cctcctcggt cgttacggga gatccggcaa taatctgccg ggccctcctg atctcggcct
      421 tcctggtcat caccacgccc atctcggccc taactatcgg gcgcgccgcc tacctgcgcg
      481 gcgagaggat gaagaacccc ggcgcccgcg acgagtcagg gaagggcctc tccagggccg
      541 agagctgatc gcctgcggcg attctcgaaa aggcagcagg tctcaggctt cggaagaatc
      601 gcctgttagc tctgttgcct gtcctcggat ctttccatcc gcagatcgct tcgaggcgcg
      661 gaaggcggag gtttactccg cctttcgtct caagcttcgt cggcggcatg aactaccagg
      721 actggccctt ccacggccct cagcacgttc atggagacgc tgccgagcgc gaagcgcgtg
      781 actgcccccc ggccccgcga accgaccacc acgagggccg gtccatcctc ttccgcggcg
      841 tcctggatca ccgccgccga gtctcccagg accgcctgcc tctccggacg aaccccgatc
      901 gtgtcttcca gcccggtagc caaccgggag agcgcctccc gcgcctcctc gctggcccgt
      961 tcggcaccgg cgcccggggc gtgggtcctc cctcctgtca ggggtactgg gttctggtac
     1021 gccgtgacca gggccacctc caccccaagg gcaccggcga ggccggcacc cagctcggcg
     1081 gcccttctcg cctcctcgga ggcgtcagcc ccgacgacca ccctggacgg tggccaggcc
     1141 cgctcgccgc cccgcatcac gagcaccggg caaggtgaga ggctcacgac gccctccgag
     1201 acgctgccca tgacgaggcg ctttacagcc ccgagccccc ggctgccgat gacgaggagg
     1261 tccgcgccaa gctcactggc caggtccgtg atctcctcgg caggacggcc tcttctgttg
     1321 tgggtaccgg caacggatgc tccggccgct tccgcaaggg ccgcctgctc cttcaagacc
     1381 tcctcggcag ccctctccca ctgctgtctg gcctccgagt agtcgccgct catcgcggag
     1441 gtcaggctag ggggcgtctc gtcctgccag gcgtgtacca cgtgtagctc ggcccctgta
     1501 cggttcgaca ggtcggacgc ggcccggata gccagggccg aatcttcaga gccatccacg
     1561 ccaaccagaa ccctgttcaa gggtccgctc gatgaatggt ccatcccacc actcccctcc
     1621 tcgacggtct actgttcctg cacgtccccc atgcgttggc ctcacgttac tggaaacata
     1681 tctcccccgc aaccgcgggt ggagaccgcg atttcccggc cggagacgtg cccgcgagcg
     1741 tccacatgcg acactatcgg cgatggccca ctacaaggag agacccaaca gatgatcacc
     1801 ctggcgttgc agggcgctat cgtcgtgttc ctgatcctcg tcaacggcct gttctcaatg
     1861 tcggagaccg cgctggtctc gtcgcgcaag gccgtgctca ggcagcgcgc cgagtcgggg
     1921 gacgccgggg cactctacgc gctggatctc gcagaatcgc cgaaccgctt tctctccacc
     1981 gtgcagatcg ggatctccct catcggggtc ctctccggcg ctctgggagg ggcgacgttg
     2041 tccaggccgt tagcgggctt gctctcggag accttcccgg gcctcgctcc ttacgccggg
     2101 gtcatcacct tcggcgtggt ggttggctcc atcacctacc ttaccctgat cctgggcgag
     2161 ctcgtgccca aacgcctggc cctgaacggc cccgagaagg tggcctcgcg cgtggccggg
     2221 ccgatgcgtc tcctctccac ccttgccgcc ccggcggtct ggttcctggg cgtctcgacg
     2281 gacacggtgc tgaggttgtt gagggttaga ccctcagacg agcccccggt cagcgagagg
     2341 gaggtggagg tcatgctgga agagggggca cgtcagtcgc ccggcaaact gctctagacc
     2401 ccgacaacca gcatctcgct ctggcgaccc ggtgttaggt gtggggtact ctcttgcgga
     2461 tgtgggggtc gagatcgccg gctcctctct cccgccgttc tcgacagaaa ctccaagaag
     2521 ggctcccgtc ccacgccgac gagatccgcc gacgtcccgg cgtccacgcc gggcaagggg
     2581 tccagcagca ccatatccac gtcggcgcaa ggacgctgga gcaccgagac gagccccgtg
     2641 tccaccgtct gttcgacccg ccaaccgacg tggggcgcgc cgagatcgcg gaacatcgcg
     2701 gcctcttctt cgaagctgaa gaccggcagg gccgttcctc ctgtagccag gctcgtagtc
     2761 atgatgtaga tccgcccggc ttcgaccttc acgagcagcc agaacggttt tgcagcaagg
     2821 acgttcccag cttctcccat cctaaccggc gtcttcgctg gaccccaggc cctggccgct
     2881 gtggccagtc gctccctctc tacctcgtgc accgtcgtac tgtggcggtc tttctgtaac
     2941 tgcaggaaca taagctcgtg catcttcgag aacctcctct ttacccaggc ttcgtgccgt
     3001 tggggccgtt ccatctcggg tctacgccta gcgtgtgggg cagggccgcc tcgctcacgg
     3061 tgttcggcgg gtggaggccc cctcgaacct gaggtcccgc cgcacctgct ctctcacgcc
     3121 ctcgaggatc ctggggtcgg agagtatgcg ttcgatctcc tcctgcgtgg ccatccggcg
     3181 cacgtactct tccctgatca cctcctgcaa gggggccccg tcgtcaaccc ggtgggcgac
     3241 gtattcgagg accttctccc tgcctcccca gccgtgagat gagccaaaga gcctggccca
     3301 gatagccttc cagctctcac ataacagaac ctccttcgtc tccggcctcc ccgcctggtg
     3361 gcctgtttgt ctactcccta gtctggaccg gagacgtgtg gtcggggtgt gaggatcatg
     3421 agagtttcca cagaagcttg ccggatgggg tagttgggga gaccgggggc atgatggggc
     3481 gaccgctctt cgagatccgg tcacgcaccc gtcttctgct atctgaccat cccacgggcg
     3541 gccacgggga ctctttccgt gccaccgtcc gcgtaaagca tgaagagttc atcgtgcagg
     3601 ttcaccgtgc tacagacgtg gttggggacg atctcgaccg tgtcgcccac ccgcaggccg
     3661 ctgtccgcat ccaccgagag catcccgtgt tcctcggtga ccctctccag caccgccccg
     3721 gggtggccca ccacatggcc gaaaccggtc aggtcgaggg gggcgacctt cggctggacg
     3781 tcggtggcga aggtcttgct cccgccgtcg atgatggcga ggtctggcct tggcctgctg
     3841 acaacggtga ccagcacgcg cagggcgcag tccgagagcg agcagacgcc aagcgcggcc
     3901 tgcatcctgt cgtagaagat gtaggtgcct ggcctgatct ccgtgatgcc atctaccccg
     3961 gcggcatgcc cggcggtcgg ggtagaccct acgctgacgt cctctacagg caccccggcg
     4021 tccctgatct cctgggcgac cgaggccatc agttcgccct ccaggcgccc cgccgcttcg
     4081 aggtccgtgg ttggcgagcc gtcgcccaga acggctccgc ggtaggtata gacgccggtg
     4141 aggtccaggt tctcgagcga cgagatctcc cgcgccagcc ccgctgcctc cgcgggcgac
     4201 acccccgtcc gcccgagacc ggaatcaacc tccaggcgca cctccagggt aacgcccgcg
     4261 tcccccgcca gggaggacag cctgtgggcc ccatgccggc catcgacgcc aaggatcagg
     4321 cgcatgcccc ttcgcgtcag gccgatggca cggcctatct ttatctccgt caccagcggg
     4381 taggcgacga agatgtcgtc caggccgccg tcggccatga cctcggcctc ggagatcgtc
     4441 gcgaccgtga tgcctgccgc gcccgcctcc atctgtcgcc tggcaaggga agggatcttg
     4501 tgcgtcttca cgtgcggcct gagggcgacc ccccggctct cggcaacagc ggccatcccg
     4561 aggaggttct gctccagtct ctgcgcatcg acgacgaggg cgggcgtgtc caggttcagg
     4621 tccacgccat ctccggccga cggcctatcg tcagccctca tgcggattct ctctctgcga
     4681 gatcgtcgtc aggcgccggt cctggtcgtc tgggactccg actcgtcagg ccaacggccg
     4741 ccgttgtacc agtaggccct gatggcctcg agctggcgtc ctttggtctc gggggccagg
     4801 ttgtagacga agacgattgc gaccacggac aacaggagga agatgccgaa ggtcatggag
     4861 ccgcctagag cgccaagcgc gctcaggaac acaacgccca cgatgaagtt ggcgaagagg
     4921 tccgcggtca gcatggccga gccgccctgg gtgcggagcc gtgcggggaa gctctccgag
     4981 gcgtagaccc acaccagcga cccgtatccg aagttgaagc cgaccgtgaa cagcaagatg
     5041 ccgaccaggg tgaacgccgc cgaaaggccc aggccgtagg tgaagactag aagcaagttg
     5101 gcgaaggcca tggtgcctat gccggtgagc agcgtgggcc tcctgcccca ccggtccacc
     5161 accagaaagg cgacaacctc cgcgatcacg ccggctatct gcacgaagcc ggtggcgatt
     5221 atggcgcccg tcgggctcgt gacgccgacc tgctggatta tggtcgggct gtagtagacg
     5281 atggcgttta tgcccgtgat ctggaccagg aaaccaaggc ccaccacgaa gatacccgcc
     5341 cttctgaagc gcccctggaa gagctccgcg aagctcccct tctcctcgta ggagaggtct
     5401 tcttcgatca ggtcggcctc cctttccgag tccatatcag ggttgaccct gcgcagcagg
     5461 tccacagcct cctcgcgctg gccgttcatc agtagccagc gggcggtatc gggcaggcgg
     5521 accacgagga gtagcactat caccgccggt atgacggata gagagaggat gaggcgccag
     5581 ctctctgtta ccgcgagcgc cgcgccgacg aagtaggcga tggcgatacc cgaggtggtg
     5641 g
//
LOCUS       DBNGVD010000060         5327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3288950, whole genome shotgun sequence.
ACCESSION   DBNGVD010000060 DBNGVD010000000
VERSION     DBNGVD010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3288950"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="AC16CM_01715"
     CDS             complement(<1..596)
                     /locus_tag="AC16CM_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIR3138573.1"
                     /translation="MASVKLPDGRQLEIEPGQKASEVAEMIGKRLARDAVVAKLDGEL
                     IDLDAPLNGGGDFEVVTRESADGLEVLRHSTAHAMAQAITEPYPGSRLTLGPPIENGF
                     FYDIEVAGRITDEDLPRIEERMREIVARDLPIEREEVSKAEAEALYQDNPYKTEIIEA
                     LEDGDITVYKQGDFFDLCRGPHVPSTGRLGAFKLQNIAG"
     gene            complement(684..758)
                     /locus_tag="AC16CM_01720"
     tRNA            complement(684..758)
                     /locus_tag="AC16CM_01720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Val,seq:gac)
     gene            complement(793..863)
                     /locus_tag="AC16CM_01725"
     tRNA            complement(793..863)
                     /locus_tag="AC16CM_01725"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Cys,seq:gca)
     gene            complement(897..969)
                     /locus_tag="AC16CM_01730"
     tRNA            complement(897..969)
                     /locus_tag="AC16CM_01730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Gly,seq:gcc)
     gene            complement(1003..1467)
                     /locus_tag="AC16CM_01735"
     CDS             complement(1003..1467)
                     /locus_tag="AC16CM_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR3138574.1"
                     /translation="MSEAGAKLKSGGKRFSKFTIVGLSNAAIDIGVLNLLLWLEPTRE
                     VSLLVLYNGAALILANLNSYLWNTLWTFRGRAEHDKRQILLFALQAIANVAISNGLFW
                     ALIHPIVAHTDVSMFLAGNVAKIVSVTVASTISFFIMRYVVFSRRRWSKDRL"
     gene            complement(1464..2585)
                     /locus_tag="AC16CM_01740"
     CDS             complement(1464..2585)
                     /locus_tag="AC16CM_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3138575.1"
                     /translation="MRIAFFTETFLPATDGVVTRLKHTIHELLAVGDEMLVIAPKYGE
                     GPGSFEGVPIHRVSSVPFPPYPQIKISPVNPGVGLALSRFGPDLIHAVNPFVLGWGAP
                     FFARRLGVPLVASYHTNVAAYASFYGLDLFDRVTRWHTRRLHNRAALNLCTSEATLHY
                     LQQEGIERLRLWPQGVDATHFNPDRCSKDWRVRLTGGHPEDRLLIYVGRLSHEKDIGR
                     LRLVLDEVPGVRLALVGDGPARRDLEAEFAGTPSVFTGVLKGDELATAFASADAFVFP
                     STTETLGMAMIEALASGLPVLAARAGATAEVVSDGETGLLYDPTSGASLVAVVRRIVS
                     DDGLRARMGESARAAAVCRDWRSSTQTLRGYYEEALGQA"
     gene            complement(2609..3613)
                     /locus_tag="AC16CM_01745"
     CDS             complement(2609..3613)
                     /locus_tag="AC16CM_01745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3138576.1"
                     /translation="MAQTVTLIPGDGIGPDLTDSVKEVIGALDVDIEWEIAEAGETVM
                     EEEGTPLPESVLESIERNKVALKGPLTTPVGTGFRSVNVALRKELDLYGNIRPSLSLP
                     GVETPYKNIDIVIYRENTEDLYAGVEHTVGKHAAESIKIITREGTERICRMAFERSRE
                     QGRKHITVVHKANIMKFTDGLFRDVFFEVAKEYENDFEEIDDRIVDNMCMQLVMKPNL
                     YDVLVCPNLYGDILSDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAGQNRANP
                     LATLLSEKNMLVHLGYEGEADRMQAGIEEALKSPETRTKDLGGSAGTREFTQAIVSNL
                     "
     gene            complement(3766..4350)
                     /locus_tag="AC16CM_01750"
     CDS             complement(3766..4350)
                     /locus_tag="AC16CM_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3138577.1"
                     /translation="MPSFVQTVREVLFNPVGFFRGIRRQGDYLNPLIFAIICSLITAI
                     IGGFLGLIISLISGEGIGSSIAGLFGNIIGIPIATTIGLFIGAGIYHLLVLLFVRPAH
                     AGYEATFRAVAYAQAVQAVAFLAFIPILGLLVILVIAVYQVVLNVIAIREMHATTTGR
                     AVAVVLVPVAIVLLLGLLLVGTILAIFAAAMSQS"
     gene            4641..>5327
                     /locus_tag="AC16CM_01755"
     CDS             4641..>5327
                     /locus_tag="AC16CM_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138578.1"
                     /translation="MSSTIETLERDLGEAQEEREAEQRAVVRAENAHEAACSARDEAW
                     TEAPADDPEVAELTALVYLRRRELYDAIDRLKAAEGKERRIKSRLMYVLPAERRRYLS
                     GTVDDEFEALLEAIRQAYMGSLPEASISVVTEKPGEVDLRGDATWRALERLYSGTVRR
                     WVEIRSMRDPAEAGRMARQVSYYAGGHTPRTFVGHEEVRTGTFRGLEELRPLAGYETE
                     EGKLSLARITT"
BASE COUNT         1020 a         1701 c         1643 g          963 t
ORIGIN      
        1 cccgctatgt tctggagctt gaaggcgccg aggcggccgg tgctcgggac gtgcgggccg
       61 cggcacaggt cgaaaaagtc gccctgcttg tagaccgtga tatctccatc ttccagggcc
      121 tcgatgatct ccgttttgta cgggttgtcc tggtacaggg cctcggcctc ggcctttgag
      181 acctcttccc gctctatcgg aaggtcgcgc gccacgatct cgcgcatccg ctcctctatg
      241 cgcggcagat cctcgtcagt aattcgtccc gccacctcga tgtcgtagaa gaagccgttc
      301 tctatgggtg gtcccagcgt cagcctgctc cccggatacg gctccgttat ggcctgcgcc
      361 attgcgtgcg ccgttgagtg gcgcaacacc tcgaggccgt cggccgattc cctggttacc
      421 acctcgaagt cccccccacc gttcagcggg gcgtcgaggt cgatcagctc cccatccagc
      481 ttcgcgacaa cggcgtcgcg cgcgagccgc ttgccgatca tctcggctac ctcggaagcc
      541 ttctggcccg gctctatctc cagttgtcta ccgtcgggta gtttgacgga ggccatgtgc
      601 gcacctttct gataaaggaa gaggggtaac gggtaggtcg actaggtagt tcggcgcaaa
      661 ggagcggctg ataccgcgca gcgtggtggg cgatagtgga ttcgaaccac tgacctcttc
      721 cgcgtcaagg aagcgctctc cccctgagct aatcgccctc agttaccgcg ggccccgctc
      781 caaccctccg agaggcggca cccggaatcg aaccggggta cagggttttg cagacccttg
      841 cctaaccact cggccatgcc gccagcggca ccctgcgccg caaaccgcgg cccgagagcg
      901 gaagacggga ttcgaacccg cgaccctcac catggcaagg tgatgctcta ccagctgagc
      961 tacttccgcg ctccgaataa atttagcacg ggctcatggc tcttacaagc ggtccttcga
     1021 ccagcgacgg cgagaaaaaa ccacgtagcg catgatgaaa aagctaatgg tcgaagccac
     1081 cgtcacggag acgatcttgg ccacgtttcc tgctaggaac atcgacacat ctgtatgggc
     1141 cactatcgga tgaatgaggg cccagaacag cccgttgctg atcgccacgt tggcgattgc
     1201 ctgcagggcg aacagcaaga tctgccgctt gtcgtgctcg gccctccccc tgaaggtcca
     1261 gagcgtgttc cataggtagc tgttgaggtt ggccaggatg agcgcggcgc cgttgtacag
     1321 tacgagaagc gaaacctcac gcgtgggctc cagccacaga agcaggttca acaccccgat
     1381 gtcgatggcg gcgtttgaga gcccgacgat ggtgaacttg gagaaccgct tgcccccgct
     1441 cttgagtttg gcaccggcct cgctcacgcc tgccccagtg cctcttcgta atacccgcgc
     1501 aacgtctgcg tcgacgacct ccagtcgcgg cacaccgcgg ccgcgcgggc gctctcgccc
     1561 atccttgcgc gcaggccatc gtcggagacg attctccgga cgaccgcgac gagagaggcc
     1621 ccggaggtgg gatcgtagag caggccagtc tcgccatcgg agaccacctc ggccgtggcg
     1681 cccgccctcg cggcgagcac gggcaggccg gaggccagcg cctcgatcat tgccatccca
     1741 agcgtctcgg tggtggacgg aaacacgaag gcgtcggcgg aggcgaaagc cgtcgccagc
     1801 tcgtcgccct tcagtacgcc cgtaaacacg ctcggcgtgc cggcgaactc cgcttcgagg
     1861 tcccgtcgcg cgggtccgtc tccgaccagg gcgaggcgca cccctggtac ctcgtcgagt
     1921 accagcctca acctgccgat gtccttctcg tggctcaggc gaccgacgta tataagcagc
     1981 cggtcttcag gatggccgcc cgtcagccgg acccgccagt ccttcgagca cctgtcgggg
     2041 ttgaagtgcg tggcgtcgac accctggggc cacagacgta gcctttcgat gccctcctgt
     2101 tggaggtagt gaagcgtcgc ctccgaggtg cagaggttca aggcggcgcg attgtgcagc
     2161 cgcctggtat gccagcgcgt aacccggtcg aacaggtcga ggccgtagaa actggcgtag
     2221 gcggccacgt tcgtatggta ggaggccacg agcgggacgc ccagcctgcg ggcgaagaac
     2281 ggcgcgcccc aaccgagaac gaacgggttc acggcatgga tcaggtctgg cccgaatcgg
     2341 ctcagcgcga ggcccacgcc ggggtttacg ggggagatct ttatctgggg gtagggcgga
     2401 aaaggcacgc tcgagacccg gtggatcggc acgccctcga aagaccccgg cccctccccg
     2461 tatttgggcg cgatgaccag catctcgtcg cccaccgcga gcaactcgtg aatggtgtgc
     2521 ttgagcctcg tcacgacccc gtcagtggct ggcagaaacg tctcggtgaa gaacgcgatc
     2581 cgcaaacgct tctccctggc tcgatggcct agaggttgga gacgatggcc tgggtgaact
     2641 ccctggtccc cgcgctaccg cccagatcct tggtgcgggt ttcggggctc ttcagggcct
     2701 cctctatacc ggcctgcatc ctatcggcct cgccttcgta gcccagatgc acgagcatgt
     2761 tcttttccga gagcaacgtg gcgagcgggt tggcgcggtt ctggccagca tacttgggag
     2821 ccgagccgtg aaccggctcg aagacggcga tctcctcccc gatgttcgcc cctggtgcga
     2881 caccaaggcc gccggtgagc ccggcgcaca ggtcggacag gatgtcgccg tagaggttcg
     2941 ggcagacgag cacgtcatag aggttcggct tcatgacgag ctgcatgcac atgttgtcca
     3001 cgatccggtc gtctatctcc tcgaagtcgt tctcgtattc ttttgcgacc tcgaagaaca
     3061 cgtccctgaa caggccgtcc gtgaacttca tgatgtttgc cttgtggacc acggtgatgt
     3121 gcttgcggcc ctgttctctg gaccgctcga aggccatccg gcagatacgc tcggttccct
     3181 cgcgggtgat gatcttgatc gactcagccg cgtgcttgcc gactgtgtgc tccacgccgg
     3241 cgtagaggtc ctcagtgttc tcgcggtaga tgacgatgtc gatgttcttg tagggcgtct
     3301 caacaccggg caggctcaac gacggcctga tgttcccata caggtccagc tccttgcgca
     3361 gcgccacgtt cacagaccgg aaacctgtac cgaccggcgt ggtcaggggc cccttgaggg
     3421 ccaccttgtt gcgctcgatc gactcgagca ccgactccgg taggggcgtg ccctcctctt
     3481 ccatcaccgt ctcgccggct tcggcgatct cccactcgat atccacgtcg agcgcgccga
     3541 tgacctcctt gacggagtcc gtcaggtccg gcccgatgcc gtcgcccggt attagagtaa
     3601 ccgtctgcgc cacgcaatgc ccccttcact agtaaaagcc ggcattagta tagcggccgt
     3661 agacgaaaag ttcggaagcg tatcgggggc ccagatggac ccccgatacg ccggttattc
     3721 atgctgccct agctatatgg ccacgaacgt gttcgaaacc tcctactagg actggctcat
     3781 ggctgcggcg aagatggcca gaatggtgcc aacgagcaag agccccagga ggagcacgat
     3841 ggccacggga accagcacaa ccgcgacggc ccgaccggtg gtcgtggcgt gcatctcacg
     3901 gatggcgatc acattcaaaa ccacctggta gacagcgata accagaatca ccaggaggcc
     3961 caggataggt ataaacgcca ggaaggccac agcttggacc gcctgggcat aggccaccgc
     4021 cctgaaagtt gcctcgtatc cggcgtgtgc gggcctaaca aacagaagca cgagcaggtg
     4081 gtagatgcca gccccgatga acaggccgat cgtagtggcg attgggatcc ctattatgtt
     4141 accgaacaga ccagcaatag aagagccgat tccctcaccc gagatcaggg agataattag
     4201 gccgagaaag ccaccgatta tggcggtgat gagtgagcag atgatcgcaa agatcagcgg
     4261 gttcaggtag tcgccctgcc tgcgaatgcc cctgaagaaa cctaccgggt taaagaggac
     4321 ttcccgtacc gtctgaacga aacttggcac aaggttcctg tagtcgaaat cggttccccc
     4381 cgatacgcct ccagacactc taggaggcgg tggaacacca gctcccccag atgagccgcc
     4441 cgtccctgat cccccgccag atcccgtgtt gaagtccacg cgaatctcct ctccgatcgc
     4501 cctgcggata actacttgca atgtacatgt tgttaacaat ttgcgcaacc gggcagaggc
     4561 ctgcacccct cttcatggcc cggtggtgtg ctgtagaatg acctcgtggg agcagcaagt
     4621 atgggaacgg gaaagtcctt atgtcgtcta ccatagagac cctcgaacgc gatctgggcg
     4681 aggcgcagga agagcgagag gcggaacagc gtgccgtcgt gcgcgccgag aacgcgcacg
     4741 aggccgcatg ttcggcgcgt gatgaggcct ggacggaggc cccggcggac gaccccgagg
     4801 ttgcggagct gaccgccctc gtctacctca gacgccggga gctctatgac gccatagaca
     4861 ggctcaaggc tgccgagggc aaggagcgca ggataaagag ccgcctgatg tatgtgctcc
     4921 ccgccgaacg ccgccggtac ctgtctggaa cggtcgacga tgagttcgag gccctgctcg
     4981 aggcgatcag gcaggcgtac atgggttcgc tgccagaagc gagcatctcc gtggtgaccg
     5041 aaaagcccgg tgaggtcgac ctgcgcggag acgcaacctg gcgcgccctg gagcgtctct
     5101 actcgggcac cgtgcgcagg tgggtggaga taaggagcat gcgcgacccc gccgaggctg
     5161 gccggatggc gcgccaggtc tcctactacg cggggggcca taccccacgt accttcgttg
     5221 gccacgagga agtcaggact ggcacgttcc gcggcctcga ggagctcagg ccgctcgccg
     5281 gctacgagac cgaagaagga aaactctcgc tcgcccgcat caccacc
//
LOCUS       DBNGVD010000061         8709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4746610, whole genome shotgun sequence.
ACCESSION   DBNGVD010000061 DBNGVD010000000
VERSION     DBNGVD010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4746610"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16CM_01760"
     CDS             complement(<1..644)
                     /locus_tag="AC16CM_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3138579.1"
                     /translation="MSIIDNTQEHSTRIFLVEDHASFRQALAFMFERQGEFAVVGQAG
                     SLAEAHAFLRRSPDDFDIAVVDLALPDGDGFGLIAELSSRPAVMTLVLSASLEPARFA
                     RAVEAGASGVLHKSAPINEIVDAVRRLRSGEALLTPAETVEMLRMVSRRRQEEYEARR
                     AIEKLTPREKQVLAALGEGLDSRDIAEKLHITIETERTHMVNILGKLDVHSRLQA"
     gene            complement(726..3677)
                     /locus_tag="AC16CM_01765"
     CDS             complement(726..3677)
                     /locus_tag="AC16CM_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3138580.1"
                     /translation="MREARPRKASATRTLRVLLVEDSENDAMLLLRELRRGGYEPVFE
                     RVHTPEGMEEAWRLAEAGGRPFQIVISDYFLPRFGAAAALELSRKLAPDVPFIVVSGK
                     VGEEAAVGIMKAGADDYLAKEDMSRLCPAIERELREAEVKRERELALTRSEDRFKRLV
                     EQAADAIFVHDLEGRLVDVNRQACVSLGYTREELLGMSVADVETSFRAGSLGKLWKQI
                     SSGTPLTLEGAHRRKDGTTFPVEIRVGLFEADERPLMLALVRDVSGRREAEEKIRETE
                     ARYRTLVERIPAVTYVQEPLESDNPKAITYMSPQYEAMLGYPADAEIVDEEHWLKTLH
                     PEDRARVLAEEVRTDETGEPYRVEYRQIARDGSVVWVRDEADLLRDGDGRPLYWLGVQ
                     YDITEQKRTEQELRESEERFRATFEQAAVGILQVGLDGGWLRFNDKFCDITGYPREEL
                     GAASVFSLIGPEDFDRDFERGLSMLAGELRDYSEEKLIIGKGGVRVWIDLTVSLVYDE
                     NDEPIYFIAVVEDIGKRKEAEEGLRQSEERFRMLSEEVVEGLVLSENGRIIDANRSLT
                     EMFGYELAELVGIEAGDLTPPDRREMVRRRIEREDTSYYESRGQKKDGTVFPIEIRSR
                     KLPYSGRRIRVTSVIDLTERKRQEEALRQSEALYRTVVEQAAENIFLVDARTRRVLEA
                     NDALLHSLGYTEDELKRMTLYDIVAHERESVDLNIGRVMEEGRASLGERRYRRKDGTL
                     ADVEVNVSAVPYDGRRVLCVVAHDVTQRKRAEAMLEEIREAERNRIARELHDGTLQDI
                     VYALQELQVMQVMAGDGGNPSLENTVDALRRSVEGLRSAIFELRLEGTLDRSIVTSLE
                     NLIDLNRRMARERYDIELVVGDDFPDDLPEMAGQEITRLVQEALTNIRRHAVASHARV
                     ELGTDGGLAFVEVSDDGRGFDPNEEGTGIGRQSMGQRALELGGGLDLQSAPGEGTTVR
                     LLVPLSRLMKE"
     gene            complement(3670..5514)
                     /locus_tag="AC16CM_01770"
     CDS             complement(3670..5514)
                     /locus_tag="AC16CM_01770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3138581.1"
                     /translation="MRSWPGRVFRHVRIAATAYAVLLLALLLTGLAWYYVRATVKEQN
                     NVRFDETVQATRAAVDRRTDAYLDALFGARGVFLASNAVERGEWESYVNGIETKTRLE
                     GLQALGFARYISPEEQEDFERRAREQGQPEMRPDLDPGGERSAYFPLTLVAPLDRANL
                     NLINHDYYTDPAHRTAMDQARDSGSPRATRLVYVLTEPPARSSADLAFGPGFAVYLPV
                     YDESRPTDTRAERRKALKGFVVGYFRRDGLFEDVFGSGFDPAIDFEVYDGANAGSSSL
                     LYDDDGVQRAGDNEFEPLFSRQERSEVAGRRWSLYFANQPGFNKGAESNLPAFVLASG
                     IGLSLLLFGISWMLVRSRLLAEDASRDLEDANRELEGANRELEAFSYSVSHDLRAPLR
                     TIDGFSQILQEDYEAVLDEEGLDYLGRVRAASAHMAMLIDDLLDLSRVSRRPLRRESV
                     DLADLAAEVIEDLREAEPERDVEFVVGRGSRAYGDMGLLKVALENLIGNAWKFTKREE
                     RARIEFGVDGRPRTGVAGPAFFVRDNGAGFDQAYADKLFGAFQRLHGQDEFEGTGIGL
                     ATVARIIHRHGGRVWAEGEVGEGATFYFTLGGREQGDRSAPARKAEHA"
     gene            complement(5807..6337)
                     /locus_tag="AC16CM_01775"
     CDS             complement(5807..6337)
                     /locus_tag="AC16CM_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138582.1"
                     /translation="MSQLPSMGYGLLPYLALACVSTLIGYYFARKRTEYEVGYSHRVE
                     VYEGVQGMVIPLVEEFEAALEYVRAPGPSGELPVEDIENSVDGLEKYVAQHEMWLDRR
                     TSAAVGDLITAFRARLGNLELLPDRYDDPGFERAYERTASDLDAWLGTDLPAAREDLD
                     DAFRAMLGVGRWRRWL"
     gene            complement(6334..6984)
                     /gene="lexA"
                     /locus_tag="AC16CM_01780"
     CDS             complement(6334..6984)
                     /gene="lexA"
                     /locus_tag="AC16CM_01780"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3138583.1"
                     /translation="MEESLHPRREKILRFLARRAGGGEPPPSVREVGVEVGLKSSQTV
                     HHHLRKLEEEGYVTRESGARMVRLTEKGWGVAGRMGLLGRIAAGRGLEAVATEEAYSL
                     AAELLASRSGKRRYLLRVVGRSMVGARIEDGDLLVVEEDESPSEGSIVVVLLREGEEV
                     TVKRFYREGEMLRLKPEAPQGGYEDMVVPAEEARVQGRVVYVLHPPGRKSRGGGRG"
     gene            complement(7094..8029)
                     /locus_tag="AC16CM_01785"
     CDS             complement(7094..8029)
                     /locus_tag="AC16CM_01785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138584.1"
                     /translation="MRVPEREDAGEVAAMIRAADLADNGTSDMSVEELLDDWHSLDLT
                     QEAVAVVAPEGTIAGYADVVNRSYVSVSVYGYVHPDFRGLGIGKSLISWGERWTRERM
                     PHAPEDARVVIQHYVNDLNEPAQRLLRSLDYAPVRGVYVMETELHEAPPQPLWPEGVT
                     VRTFTPGKDERPLFEAVEDAFRDMWGRPRGTLERFISMTQVESFDPTLWFLAVDGDEI
                     AGATLCKTLSGEGWIEVVGVRRPWRNRGLGLALLRHALTEYHRRGIHKVALSVDAESI
                     TGAPRLYGRAGMSVRESYIIYLKELRPGKDLGVRS"
     gene            complement(8117..>8709)
                     /locus_tag="AC16CM_01790"
     CDS             complement(8117..>8709)
                     /locus_tag="AC16CM_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3138585.1"
                     /translation="LVRELSTGPKRFKDLLAGLPGIGTNLLAARLKRLEGEGIVGRGT
                     LPPPAGSNVYELTGLGKDLEPVLVALSRWGARLLEDPGEGDEVRAGWAAVALRSAVPA
                     GSQDGRPGTYEFSIDGEAFYLRVAGGEVEVRQGPAPDPDLVVQGDTGTLLAVASGRIS
                     PEEAVRSGALGVQGEREGDREALLSWCRHLVPPSAG"
BASE COUNT         1398 a         3125 c         2627 g         1559 t
ORIGIN      
        1 gcctgcagcc tggagtggac gtccagcttg cccaggatgt tgaccatgtg ggtgcgctcg
       61 gtctcgatgg tgatgtgcag cttctcggcg atatctcggc tgtctagccc ctcgccaagg
      121 gccgctagca cctgtttttc gcgaggggtg agcttctcga tggccctcct cgcctcgtac
      181 tcctcctgac gcctgcggct gaccatacgc aacatctcga cggtctcggc cggggtgagg
      241 agggcctccc ccgacctcag gcgtcgcacg gcgtcgacga tctcgtttat cggggcggac
      301 ttgtggagca cgcccgaggc cccggcctcg actgccctgg cgaaccgggc gggctccagg
      361 ctggcgctta gaaccagggt catgacggcg ggacgggaag agagctccgc gatcagcccg
      421 aacccgtccc cgtctggcag agccaggtcg acgacggcga tgtcgaagtc gtcaggagac
      481 cggcgcaaaa aggcgtgcgc ctcggccagc gatcccgcct gcccgaccac ggcgaactct
      541 ccctgccgct cgaacatgaa ggccagcgcc tggcggaacg atgcgtggtc ttcgacgagg
      601 aatattctgg tcgaatgctc ctgcgtgttg tctatgatgg acacccttct tctctgcccc
      661 ccggccacga tgctcgcaca acatcttacc ctagcctgaa cgggccgtga atctgggccc
      721 ggcccctatt ctttcatcag gcgggagagt ggcacgagga ggcgcaccgt cgtgccctcg
      781 cccggcgcgc tctgcaagtc gagcccgccg ccgagctcga gcgcgcgctg gcccatagac
      841 tgccggccga tccccgtccc ctcctcgttt gggtcgaagc cgcgaccgtc gtcggagacc
      901 tcgacgaagg cgagcccgcc gtccgtcccg agctccaccc tcgcgtggga agccacggcg
      961 tggcggcgga tgttcgtgag cgcctcctgg acgaggcgtg tgatctcctg ccccgccatc
     1021 tcgggcaggt cgtcggggaa atcgtcgccg accaccagct cgatatcgta cctctcgcgg
     1081 gccatgcgcc ggttcaggtc gatcaggttc tccagcgagg tgacgatgga gcggtcgagc
     1141 gtcccttcga ggcgtagctc gaagatggcg ctgcgcaatc cctccaccga gcgcctgagc
     1201 gcatcgaccg tgttctccag ggaagggttc ccgccgtccc cggccatgac ctgcatcacc
     1261 tgcaactcct gaagcgcata gacgatatcc tggagggtgc cgtcgtggag ttccctggct
     1321 atcctgttcc tttcggcctc cctgatctcc tccagcattg cctccgcgcg cttgcgctgg
     1381 gtgacatcgt gggcgacgac gcacaagacc cgccggccat cgtagggaac ggcgctgacg
     1441 ttgacctcca cgtcggccag cgtgccgtcc ttgcggcgat agcgtctctc gcccagcgag
     1501 gccctgcctt cctccatgac gcgcccgatg ttgaggtcaa cgctctcgcg ctcgtgggcc
     1561 acgatatcgt agagcgtcat acgcttgagc tcgtcttccg tgtagcccag agaatggagc
     1621 agggcgtcgt tcgcctctag cacgcgtctg gtccgggcgt cgacgaggaa gatattctcc
     1681 gcggcctgct cgaccaccgt gcggtagagg gcctcactct gcctaagggc ctcctcctgg
     1741 cgcttgcgct ccgtcaggtc gatcacggag gtcaccctga tgcggcgccc agaatagggc
     1801 agcttccttg accttatctc tatggggaat acggtgccgt ccttcttctg gcccctcgac
     1861 tcgtagtagc ttgtgtcctc tcgctcgata cgccgccgca ccatctccct gcggtctggc
     1921 ggcgtaaggt cgccggcttc gatgccgacc agctccgcga gctcgtaccc gaacatctcg
     1981 gtaaggctgc ggttggcgtc gatgatcctg ccgttctcgc taaggaccag gccctccacg
     2041 acctcctctg agagcatcct gaaacgctcc tcgctctgcc tgagcccttc ctcggcctcc
     2101 ttgcgtttcc cgatgtcctc cacgaccgct ataaagtaga tgggctcgtc gttctcgtcg
     2161 tagaccagcg agacggtgag gtctatccag accctcacgc cgcccttgcc gatgatgagc
     2221 ttctcctcag agtagtccct cagctccccg gccagcatgc tgaggccgcg ctcgaagtcc
     2281 cggtcgaagt cctctgggcc tatgaggctg aagacgctgg ccgccccgag ctcctcgcgc
     2341 ggatagcccg tgatgtcgca gaacttgtcg ttgaacctga gccatccccc gtcgagcccg
     2401 acctgcagga tgccgacggc ggcctgctcg aaggtggccc tgaaacgctc ctcgctctcc
     2461 ctgagctctt gctcggtccg tttctgctcc gtgatgtcgt actgtacccc gagccagtaa
     2521 aggggtcggc cgtccccatc cctgaggagg tcagcctcgt cgcgcaccca caccacgctt
     2581 ccgtcgcggg cgatctgacg gtactcaacc ctgtagggct cgccggtctc gtcggtcctg
     2641 acctcctcgg ctaacacccg cgccctgtcc tcagggtgca acgtctttag ccagtgctcc
     2701 tcgtcgacga tctcggcgtc cgccgggtat ccgagcatgg cctcgtactg cgggctcatg
     2761 tacgtgatgg cctttgggtt gtcggactcc aggggttcct ggacgtacgt cacggccggt
     2821 atccgctcga cgagcgtgcg gtagcgggcc tcggtctccc tgatcttctc ctccgcctcc
     2881 ctgcgccccg agacgtcgcg cacgagggcc agcatgaggg gccgttcatc cgcctcgaag
     2941 aggccgaccc tgatctctac ggggaacgtg gtgccgtcct tgcgacggtg tgccccttcg
     3001 agcgtgaggg gcgtgccgga ggagatctgc ttccacagct tgccgagcga ccccgccctg
     3061 aaggaggtct ccacgtccgc aacggacatg ccgaggagct cctctctggt gtacccgagg
     3121 ctcacgcagg cctggcggtt cacgtcgacg agacggcctt cgaggtcgtg cacgaagatg
     3181 gcatctgcgg cctgctcgac gagccgtttg aagcggtcct cgctcctggt gagcgcgagc
     3241 tcacgctccc gctttacctc cgcctccctg agctcgcgct cgatggcggg gcacagcctg
     3301 ctcatgtctt ctttggcgag gtagtcgtcg gccccggcct tcatgatccc aaccgccgcc
     3361 tcctcaccca ccttgccgga gacgactatg aaaggcacgt cgggggcgag ctttcgggac
     3421 aactccagcg cggcggcggc cccgaagcgg ggcaggaaat aatcggagat cacgatctgg
     3481 aagggacgcc ccccggcctc ggccagacgc caggcctcct ccatgccctc tggggtatga
     3541 accctctcga aaacgggctc gtacccgccc ctgcgtagct cccgcagaag gagcatggcg
     3601 tcgttctcgg agtcctcgac gagaagcacg cgcaaggttc tggtcgcgct agccttcctc
     3661 gggcgggcct cacgcatgct cggccttcct ggccggggcc gagcggtccc cctgttccct
     3721 gccaccgagc gtgaagtaga aggtggcccc ctcgccaacc tccccctctg cccagacccg
     3781 gccgccgtgc ctgtggataa tgcgtgccac ggtggccaac cctatgcccg tcccctcgaa
     3841 ctcgtcctgc ccgtgcagcc tctggaaggc accgaacagc ttgtccgcgt acgcctggtc
     3901 gaagccggct ccgttgtccc gcacgaaaaa ggcggggccg gcaacaccgg tccggggcct
     3961 cccgtccacc ccgaactcta tgcgcgcccg ctcctctctc ttcgtgaact tccacgcgtt
     4021 tccgatcagg ttctcgagcg ccaccttgag gaggcccata tcgccgtatg ccctgctgcc
     4081 tctccccacc acgaactcca cgtcccgctc cggctcggcc tccctcaggt cttctatgac
     4141 ctccgccgcc aggtccgcca gatctacgga ttccctgcga aggggcctgc ggcttaccct
     4201 ggagaggtcg agcaggtcgt cgattagcat ggccatgtgg gcgctggccg ccctgacgcg
     4261 gccgaggtag tcgagcccct cctcgtcgag cacggcctcg tagtcctcct gcaggatctg
     4321 ggagaacccg tctatggtcc tcaacggtgc cctcagatcg tgcgagacgg agtaggagaa
     4381 cgcctcgagt tccctgtttg caccttcgag ctccctgttg gcgtcctcca ggtccctgct
     4441 cgcgtcctcg gccaggaggc ggctgcgcac gagcatccag gagatgccga agagcaggag
     4501 gctcaggcct atgccgctgg ccagcacgaa ggccgggagg ttgctctccg cccccttgtt
     4561 gaaccccggc tggttggcga aatacaggct ccacctgcgc cccgccacct cgctccgttc
     4621 ctgccgggag aagaggggtt cgaactcatt gtccccggct cgctgcaccc cgtcatcgtc
     4681 gtagagcagc gagctggatc cagcgttggc cccgtcatag acctcgaagt ctatggccgg
     4741 gtcgaagccg ctcccgaaca cgtcctcgaa gagaccgtcc cgccggaagt acccgacgac
     4801 gaaacctttc agggccttcc gacgctcggc gcgcgtgtcg gtcggtcttg actcgtcgta
     4861 cacgggaagg tagacggcga agcccgggcc gaaggcgagg tcggcgctcg atctcgcggg
     4921 gggctcggtc agcacgtaga ccaacctggt cgcccgaggg gagcctgagt cgcgcgcctg
     4981 atccatggcc gtcctgtgcg ccgggtccgt gtagtagtcg tggtttatca ggttgaggtt
     5041 ggcacggtcc aatggcgcga cgagcgtgag cgggaagtag gcggatctct cgccgccggg
     5101 atccagatcc ggccgcatct cgggctgccc ctgttccctt gcccgccgct cgaagtcctc
     5161 ctgctcttcg gggctaatat acctggcgaa accgagcgcc tgaagccctt cgagccgggt
     5221 cttggtctca atgccgttga cgtagctctc ccactccccg cgctcgaccg cgttgctggc
     5281 gagaaacaca ccacgggccc cgaagagcgc gtcgaggtag gcgtcggtcc tcctgtccac
     5341 ggccgccctc gtggcctgca cggtctcatc gaaacggacg ttgttctgct cttttaccgt
     5401 cgcccgtacg tagtaccagg ccaaccccgt gagcaagagc gcgagcaaga gcacggcgta
     5461 ggccgtggcc gcgatgcgca cgtgacggaa cacccggccg ggccaggatc gcacggcacc
     5521 gagatagccc ctgaccgcgc ctacgagagc ccccggtccc cgagcaacac gaaacgggcg
     5581 acgcaggacg aatcccccaa cgataacgct gcgctcaccg ccctacgaac ctttccttct
     5641 acaaacgatg cccgaacggt tagcccggga cgcgccgtac agagcaatgt taaccgccgc
     5701 gtaaggaccc cgcaacagcc aagacacccg gcaacgccac acgagcacgc ccgggcattc
     5761 ggcccaggga gctgatagcc gacagctgaa ggctggaagc cgaaagctac aaccaccgac
     5821 gccagcgtcc gacaccgagc atggcgcgga acgcgtcgtc gaggtcttcg cgggcggcgg
     5881 gcaggtcggt ccccagccac gcgtcgaggt ccgaagcggt ccgttcgtag gcccgctcga
     5941 agccggggtc gtcgtatcgg tcggggagga gttccaggtt ccccagtcgg gcgcggaagg
     6001 cggtgatgag gtctcccacg gcggcggagg ttctacggtc gagccacatc tcatgctggg
     6061 cgacgtactt ctcgagaccg tccacggagt tttcgatgtc ctcgaccggc agctcccccg
     6121 acgggcccgg cgcccgcacg tactcgaggg cggcctcgaa ctcttcgacg agcggtatca
     6181 ccatcccctg cacgccctcg tagacctcga cgcgatggct gtatccgacc tcgtactcgg
     6241 tccgcttcct ggcgaagtag tacccgatga gggtgcttac acaggcgagc gccaggtaag
     6301 gcaacaaccc gtagcccatt gagggaagct ggctcacccc cgtccgcctc cccgagactt
     6361 cctccccggg ggatgcaaga cgtagaccac gcgaccctgc acccgcgcct cctccgccgg
     6421 caccaccata tcctcgtacc ctccctgagg agcctcgggc ttcagcctga gcatctcccc
     6481 ctccctgtag aagcgtttta ccgtcacctc ctcaccttcc ctgagcaaca ccacgactat
     6541 ggagccttcc gacgggctct cgtcctcctc cacgaccagc agatccccgt cctcgatcct
     6601 ggcccccacc atgcttcgtc ccaccacccg taacaggtac ctgcgcttcc ccgaacggga
     6661 cgcgagaagc tccgccgcaa gagagtaggc ctcctccgtg gcaacggcct ccagcccgcg
     6721 cccggcggcg atcctgccaa gcaaccccat ccttccggca accccccatc ccttctccgt
     6781 gagccggacc atccgcgccc cgctctcccg cgtcacgtag ccttcttcct ccaactttct
     6841 cagatggtgg tgtacggtct gagaactctt cagaccaacc tcaaccccga cctcccgtac
     6901 gctaggcggc ggctccccac ccccggcacg ccgcgccagg aaacgcaaga tcttctcacg
     6961 cctcggatgt agcgactctt ccatccaaca ccacgttagc gcaccacaca gcccgtgtca
     7021 aacaaatgtt tgatggggaa accctggccc acagttcggg aggttcgcga accgcctcta
     7081 cggatggttt gttctaggat ctgacgccga ggtctttgcc agggcgtagt tctttgaggt
     7141 agatgatgta gctctccctg acgctcatgc cggcgcgtcc gtagagcctg ggggcgcctg
     7201 tgatgctctc ggcatcgacg ctcagggcga ccttgtggat gccgcgtcgg tgatattcgg
     7261 ttagggcgtg gcggagcagg gcgagcccga gcccgcgatt tcgccaggga cggcgcacgc
     7321 cgacgacctc gatccatccc tctccggaga gtgtcttgca gagggtggcg ccggctatct
     7381 cgtcgccgtc gacggccaga aaccagaggg ttggatcgaa gctctccacc tgtgtcatgg
     7441 agatgaagcg ctcgagcgtg ccgcgcggcc ttccccacat gtccctgaaa gcgtcctcga
     7501 ctgcctcgaa gagcggacgc tcgtctttgc ctggggtgaa ggtgcgcacg gtgacgccct
     7561 ccggccagag cggctgcggc ggagcctcgt gtagctctgt ctccatcacg tacaccccgc
     7621 gcaccggagc gtagtcgagc gaccgcagta gccgctgcgc cggctcgttg aggtcattga
     7681 cgtagtgctg gatcacgacc cgcgcgtcct cgggagcgtg gggcatgcgc tcccgcgtcc
     7741 agcgctcacc ccaagagatc aggctcttgc ctatgcccag gccgcgaaag tccgggtgga
     7801 cgtagccgta aaccgagacg ctaacgtagg agcggttgac cacgtccgcg taacccgcga
     7861 tggtgccttc gggggccacg acggccacgg cctcctgggt gaggtcaagg gaatgccagt
     7921 cgtcgagcaa ctcctccacg ctcatgtcag aggtgccgtt gtcggcgaga tcggcggcgc
     7981 ggatcatcgc agccacctcc ccggcgtcct cgcgctccgg caccctcatg ccgtatcctt
     8041 gtggcaaccc tttcatggca acaaagttag cacaaacgcc cgggtcgatg gccgtcaggg
     8101 gtgctagaaa cgtcgcctat ccggcggatg gcgggaccag gtgccggcac caggagagca
     8161 gggcctcacg gtctccctcg cgctccccct ggaccccgag cgcccccgat cttaccgcct
     8221 cctctgggct tatcctgccc gaagccaccg cgagcagggt ccccgtgtcg ccctgaacga
     8281 cgaggtcggg gtcgggggcc ggtccctgcc tgacctccac ctccccaccg gccacgcgaa
     8341 ggtaaaacgc ctcgccgtcg atgctgaact cgtaggtgcc tgggcgcccg tcctgggatc
     8401 ccgcaggcac ggcggaccgc aacgccacgg cggcccaccc cgccctgacc tcgtcaccct
     8461 cgcccggatc ctcaagaagc ctggcccccc aacgcgagag cgcgacaagg acgggctcta
     8521 gatccttccc aagccccgtc agctcgtaga cgttggagcc ggccggcgga ggaagcgtcc
     8581 cccgaccgac gatcccctcc ccctccaacc gcttgagcct ggcggccagc aggttcgttc
     8641 cgattcccgg caaccccgcc aggagatcct tgaaccgctt cgggcccgta gagagctcgc
     8701 gcaccaaca
//
LOCUS       DBNGVD010000062         9998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4929286, whole genome shotgun sequence.
ACCESSION   DBNGVD010000062 DBNGVD010000000
VERSION     DBNGVD010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4929286"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1835)
                     /locus_tag="AC16CM_01795"
     CDS             complement(120..1835)
                     /locus_tag="AC16CM_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3138586.1"
                     /translation="MRSRSRTPAGGRSRGIGEILVSDGVITTGQLDEAYRLGRQQRQG
                     VGRVLLSLGYVGQSDLARAMARRLRLPFVELGTDDVDPEVAGLVERKVLRRYGMLPLR
                     VEDGRLVVAMKDPANLHAIEDLSMLSGYTISPVVVSASDLRRVFDRLFGDGDEVADLL
                     EEASAEVPKEDDENVELREHRGPTGAPTVRLVGSILRRAAGAGASDIHLEPRGEELRV
                     RFRVDGVLREVMSVPSALQGGVVARLKVLASLDIAERRLPQDGRFSATLEDGRMDMRV
                     ATLPTTFGEKVVLRLLDNADLRTDLRGLGLSRSMLRGYEKVYRRPYGTVLVTGPTGSG
                     KSTTLYATLGELNATERNIVTVEDPVEYRMRGINQVQVNPRIGLTFHAGLRSILRSDP
                     DVVMIGEIRDRETAKTSVEAALTGHLVLATLHTNNAPSAVTRLTDMGVEPFLTASAVD
                     CVIAQRLARRLCERCKRPAEVDDELIRELEPGERPSDGMKNNFHAAVGCERCSGTGYA
                     GRIGVYEIMLVDEQLRRLILRGASVDEMETVARQAGMIPLKQDGLAMAAQGITTVEEV
                     LLTVL"
     gene            complement(1936..3048)
                     /gene="mltG"
                     /locus_tag="AC16CM_01800"
     CDS             complement(1936..3048)
                     /gene="mltG"
                     /locus_tag="AC16CM_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3138587.1"
                     /translation="MRRHDKKRRDREELDARLEALGRPDRSRIRRTRRSNAGPTVFGI
                     LVVVAILGAVYLIYTAAVGGKDVRAQEGPVVVDVVKGDTLSDVADKLEAAGVIKSAFV
                     FKLQARYEGYGTEIQTGRYTFERGQDADVILRKLAAGKAAPTITVTIPEGLTLEETAR
                     TVAKDSGVSVADFEGAARETDYDYAFLEHRGIETTEGYLYPARYDFEKGVTARQVVDR
                     LLGQYLIETQSLDIPGARDRLGLTEHQLVIVASLIEREAASPREKPLVASVIYNRLDR
                     DMPLQIDATIQYALERPKENLSLDDLKIDSPYNTYENKGLPPAPICNPSRESLEAALN
                     PASTDYLYYVLKADGQSHFFTDDYQDFLREKAKAGR"
     gene            complement(3045..3470)
                     /gene="ruvX"
                     /locus_tag="AC16CM_01805"
     CDS             complement(3045..3470)
                     /gene="ruvX"
                     /locus_tag="AC16CM_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3138588.1"
                     /translation="MPGAGRGRVAALDVGDVRTGVAVSDPGRVIANPLQVVPSGELPS
                     FLDRLVEEQDVREVVVGVPRTLGGEVGFQAQKILATLDALKRDFPGLLFVEWDERLTT
                     RIAAGSRGKKGSRKRKPVRVDHLAAARMLQEYLEAGRNT"
     gene            complement(3474..4352)
                     /locus_tag="AC16CM_01810"
     CDS             complement(3474..4352)
                     /locus_tag="AC16CM_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR3138589.1"
                     /translation="MGPDRLRFDYRYGGRVTDEEIRRVQEISLQKITENQPIRYFTTT
                     LEEARNLGAMMLFGEKYSDLVRVVEVDGFSRELCGGTHVRGSAEIGAFKILSNRKHGA
                     DLYRIEVITGREALTYLTDATERAEEISATLKVEIDDLPEAVGSLRQEVQESREAARQ
                     ETLKRGLEEVGDLVEGAQQVDGTRVVTGQVVAADVRGLRQISDDIKNRLDGPSAVVLA
                     AALDGKAVLVANLHPDVSQKVEAGEIVREVSGVLGGGGGGGATMAQAGGGNLGAIPDA
                     LARVREILDQRLAGGE"
     gene            complement(4407..6125)
                     /gene="alaS"
                     /locus_tag="AC16CM_01815"
     CDS             complement(4407..6125)
                     /gene="alaS"
                     /locus_tag="AC16CM_01815"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3138590.1"
                     /translation="MQSNEIRRRFLDFFVERGHRLYPSASLIPHNDPTVLLTTAGMQQ
                     FITYFTGQDRPPTPRATSAQKCFRTQDIEEVGDESHLTFFEMLGNFSFGDYFKREAIA
                     WAWEFLTVELGLPPERLWITIFEGDEDAPEDLEAKELWMNVGVPEHKIFGLPKSENWW
                     GPPGDSGPCGPCSEIYYDYGEEYSHGDPQADPRYGPGGEEGDPRFLEIWNLVFNQYEQ
                     RKDGTLTPLAKTGIDTGMGLERTTAAVQNVPYVYQTDVYSPVYDRIRDDYKIEFGMSA
                     AVDRSLYILGDHARSMAFLIADGVRPGSTGREYVLRRIIRRAALQARLHLAKVNPQTQ
                     SPQFLASLAGEVANYMKDAYPELFNAVGDIRRVVEAEAARFLQIYDQGMYLLEEELRR
                     FDGGKFPGELAFMLHDTYGFPVDVTREVLSDRGLSLDEAGFRAAMDSQRDRAREAVQG
                     YDRVVAAFRDEDIRSRFVGYEREQVETRIVAIEPVPEADDEVYVVLAENPFYATGGGQ
                     VADEGWVSSDEGQLEVVDAIPAGDYQVLRARTERGAFSTGDAVVASINRVRRQQIEAN
                     HTATHI"
     gene            complement(6115..6927)
                     /locus_tag="AC16CM_01820"
     CDS             complement(6115..6927)
                     /locus_tag="AC16CM_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR3138591.1"
                     /translation="MSRRYRRPDKARTRRLLVRYHKQGDRGARERVIQEQLPLAEFLA
                     RKFAGRGEPLDDLVQVASVGLIKAVDRFDVDRKIEFSTYATPNILGEIKRYFRDKGWA
                     MRVPRGLQELRQSAKEVIRDETVRTGRSPSLQELAETLDADVEGVAEALTLGRAYNTA
                     SLDAPVSQDEPEGDTIIDLQADDRSQIDGLEDRLLLQEAIESLKGQQQQILRLRFNEG
                     KTQTEIADHIGVSQMHVSRLLRRAIEDLRVELEQLEEDQEETTLTPDGARAE"
     gene            complement(6924..7214)
                     /locus_tag="AC16CM_01825"
     CDS             complement(6924..7214)
                     /locus_tag="AC16CM_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138592.1"
                     /translation="MTEDQRQKEYAYLELPPREDMTSCVGLVLAGMVARARVGVGGLE
                     EAVEALEGFHSAGVSTRFRFSLADEGVLAEVEEPSGEGDEGTRWRTVVEMVS"
     gene            complement(7211..7570)
                     /locus_tag="AC16CM_01830"
     CDS             complement(7211..7570)
                     /locus_tag="AC16CM_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138593.1"
                     /translation="MASWGRVVAGVVIGVAGTIYATNEDVRKNLPRAARDLPDTIRRR
                     YQRAVSAGREASAQRRQEILHDLEEHGGGHHTARGAPPDPDAAQAAGAATEPVPDGQV
                     SESSPQAADQSRERDPD"
     gene            complement(7570..7950)
                     /locus_tag="AC16CM_01835"
     CDS             complement(7570..7950)
                     /locus_tag="AC16CM_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138594.1"
                     /translation="MELFVELMKGLLFAGIAVAFFVLAWMFLRLASIFSTTEESIKDV
                     TGEVVPLLGKTHVTVDNINSQLSQLDGAVGDVAGMTGELDQTTAVITRGVRGSVIKVS
                     SATAGLSRGISTFLGGEKRQEGEE"
     gene            complement(8021..9484)
                     /gene="mnmA"
                     /locus_tag="AC16CM_01840"
     CDS             complement(8021..9484)
                     /gene="mnmA"
                     /locus_tag="AC16CM_01840"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3138595.1"
                     /translation="MPDRYTPQVIDHLVRPRNAGEIANPSGRGESGHEACGDVASFTV
                     SISDNVLEEVRYGVYGCAACVAAGSALSELVEGRHIVEAARISKEDIQDALGGPLPLG
                     KEHALTLVLDALHKAFEDHWNQTSGEMLLKGMSTGGGSGRSVVAAMSGGVDSAVTALL
                     LEESGYDVVTVTFRLHDGEKGSRSCCSPDTVLFARDTAHRMGLPHFTLDLKELFNRRV
                     MRDFVGSYQEGRTPNPCVACNAHVKFHASAFLADQLGYHHVATGHYARVEEGPTLGRP
                     ADRSKDQTYVLWPVPQNLLARTVFPLGDHTKTEVRAIAKERGLAVAYTPESQDICFIP
                     DGNYRRFVRKKVAAEPGDVVDREGQVLGRHGGVVDFTVGQRRGIGVSAPTPLYVTEVR
                     PSSGQVVVGRKRDLEVREVTVTDLNRFLEIGDAACVQVRYNSQPVPCTVEEMADGRWK
                     ARLDEPVMGVAPGQSAVFYTADGSKVVGGGVVARGTA"
     gene            complement(9498..>9998)
                     /locus_tag="AC16CM_01845"
     CDS             complement(9498..>9998)
                     /locus_tag="AC16CM_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3138596.1"
                     /translation="PEPVVYGGGQENGIRSGTQNVAGIAGLGKAAELAREEIADRISH
                     EEALRDRILSGVEKIPDVTLNGPRGRRLSNNVHLTVSGVEAESLVLFLDATGYEVGSG
                     SACSSGGHKASPILLAMGRPEQDAFSVVRITVGKDNTAEEVDGFLDAFGAAVAQLREL
                     SPLYAG"
BASE COUNT         1628 a         3428 c         3046 g         1896 t
ORIGIN      
        1 gaaagcgagt ctggcgaagc caggcgaagc gtgctgacaa gcgaggcgaa gccgcaacgt
       61 gctgacaagc ccgcccgcag ggcggcgtgc gcacgcggag gcgaagccgc agcgtgctct
      121 cagaggaccg ttagcagtac ctcttcgacc gtggtgatgc cctgggccgc catggccaac
      181 ccgtcttgct tgaggggtat catgccggcc tgcctggcga ccgtctccat ctcatcgacg
      241 gaggcacccc tcaggatcag gcgccgcaac tgctcgtcga cgagcatgat ctcgtacacc
      301 ccgatccggc cggcgtaacc ggtcccggag cacctctcgc aacccacggc ggcgtggaag
      361 ttgttcttca tcccatcgga aggccgttcc ccgggctcga gttcccggat aagctcgtcg
      421 tccacctctg cggggcgttt gcagcgctcg cagagccttc tggcgagccg ttgggctatc
      481 acgcagtcta cggcagaggc cgtgaggaac ggctccaccc ccatgtcggt aaggcgcgtg
      541 acggcggatg gggcgttgtt ggtgtggagg gtggccagga cgagatggcc cgtgagagcc
      601 gcttctacgc tcgtcttggc cgtctcccta tctcttatct ccccgatcat gaccacgtcg
      661 gggtcggagc gcaggatgct cctgaggccg gcatggaagg tgagcccgat cctggggttg
      721 acctgcacct ggttgatccc ccgcatcctg tactcgacgg ggtcttcgac cgtgacgatg
      781 ttcctctccg ttgcgttgag ctcaccgagt gtggcgtaga gcgtggtgga cttgcccgag
      841 ccggttggcc ccgtgacaag cacggttccg tacggtcgcc ggtacacctt ctcgtacccg
      901 cgaagcatgc tccgggagag acctagcccc cgcaaatcgg tgcggagatc agcgttgtcg
      961 agcagcctta gcacgacctt ctcgccgaag gtggtgggca gcgtggcgac cctcatgtcc
     1021 atcctgccgt cctccagggt ggccgagaat cggccatcct gtggcaggcg ccgttccgct
     1081 atgtccaggc tggcgagcac cttgagcctg gccacgacgc cgccctggag ggcggagggt
     1141 acgctcatta cctccctgag cacgccgtct accctgaagc gcaccctcag ctcctccccg
     1201 cggggctcga ggtggatgtc ggaggccccg gcaccggccg ccctgcgcag gatggacccg
     1261 actagcctta cggttggagc acctgttggg ccgcggtgct cccgcaactc cacgttctca
     1321 tcgtcttcct tcgggacctc ggctgaggct tcctccagga gatcggctac ctcgtcgcca
     1381 tcgccgaaca ggcgatcgaa caccctgcgc aggtcgctcg ccgaaacgac gaccgggctg
     1441 atggtgtaac cggagagcat gctgaggtcc tctatggcgt gcaggttggc cgggtctttc
     1501 atggcgacca ccagccgtcc atcttccacg cgcaggggca gcattccgta ccggcgcaat
     1561 accttcctct ccaccagccc tgccacctcg gggtccacgt cgtccgtgcc cagctccacg
     1621 aacggcaggc gcagcctgcg ggccattgcc ctggcaagat ccgactgccc cacgtaccca
     1681 agcgagagaa gaaccctgcc gaccccctga cgctgctgcc gcccgaggcg gtaagcttca
     1741 tctagctgtc cggtggtgat gaccccgtcg gacaccagga tctcaccgat gccccgcgac
     1801 cttccgccgg ccggcgtacg gctacgagac cgcatggggc ggagcaagga ctatgtcgcg
     1861 gcggacacgg tgcacgaaag agttatcggt tcacccaacc gaaactctag cttgtggcgg
     1921 tacccttccg tcggtctagc gtcccgcctt ggccttctcc cgaagaaaat cctgatagtc
     1981 gtcggtaaag aagtggctct gcccgtcggc cttcaggacg tagtagaggt agtcggtact
     2041 cgcggggttc agtgccgcct caagggactc gcggctgggg ttgcagatgg gagccggtgg
     2101 taggcccttg ttctcgtagg tattgtacgg cgagtcgatc ttcagatcgt cgagggagag
     2161 gttctccttt ggcctctcca gggcgtactg gatcgtggcg tcgatctgga gcggcatatc
     2221 ccggtccaga cggttgtaga tcaccgacgc caccagcggc ttctcccggg ggctcgcggc
     2281 ttctctctcg ataagggagg ccacgatcac gagttgatgc tcggtcagcc ctagccggtc
     2341 tctagcccct gggatatcga ggctctgtgt ctcaatgagg tactgcccta gcagcctgtc
     2401 gaccacctga cgcgcggtga cgcccttctc gaagtcgtat ctggccgggt acagataacc
     2461 ttccgtggtc tctataccgc ggtgttcgag gaacgcgtaa tcgtagtccg tctcccgggc
     2521 cgccccctcg aagtcagcga cggaaacccc gctgtctttc gccacggttc gggcagtctc
     2581 ctccagcgtg aggccctcgg ggatggttac cgtgatggtt ggggcagcct tgccagcggc
     2641 gagcttgcgc aagatcacgt cggcatcctg gccccgctcg aaggtgtagc gtccggtctg
     2701 tatctcggtc ccgtacccct cgtagcgcgc ctgcagcttg aagacgaagg cgctctttat
     2761 tacgccggcc gcttcgagct tgtcggcgac gtccgaaagg gtgtcgccct ttaccacatc
     2821 taccacgacc ggtccctcct gcgcacgcac gtccttgccc cctaccgccg ccgtgtagat
     2881 gaggtagacg gcgcccagaa tggccacgac cactaggata ccgaaaacgg tcgggccagc
     2941 gttcgagcgg cgcgtgcggc ggatcctact gcggtccggg cgcccgaggg cttcgagtct
     3001 tgcgtccagc tcctcccggt cccgccgctt cttgtcgtgg cgcctcaagt gttccttccg
     3061 gcctcgagat actcctgaag catcctggct gccgccaggt ggtccaccct gaccggcttc
     3121 ctctttcggc tccccttctt gccacggctc ccggcggcga tgcgggtcgt gagccgctca
     3181 tcccactcca caaacaacag gccgggaaaa tcccgtttca aggcatccag tgtagccaaa
     3241 atcttctgcg cctggaatcc cacctcgcct ccgagcgtcc ttggaacccc gacgacgacc
     3301 tctcgcacat cctgttcttc gaccaggcgg tccagaaacg aggggagctc cccactcggc
     3361 accacctgaa gggggttggc gatcacacgc ccgggatcgg agacggcgac gccggtgcgt
     3421 acgtccccca catcaagggc ggcgacgcgc ccgcggccgg ccccgggcag ggcctactcc
     3481 ccgccggcga gacgctggtc caggatctcc cgcaccctgg ccagggcgtc ggggatggca
     3541 ccgagattac cgccaccagc ctgggccatg gtagcacccc ctcctcctcc accgccgagc
     3601 acacccgata cctcgcgcac tatctcccca gcctcgacct tctgtgacac gtcggggtga
     3661 aggtttgcca ccagcacggc cttcccgtcc agtgcggcgg cgagaaccac cgccgaaggt
     3721 ccgtcgagac ggttcttgat gtcgtcggag atctggcgca gccccctgac gtcggccgcg
     3781 acgacctggc ccgtcaccac cctggtgccg tcgacctgct gggcaccttc aaccaggtct
     3841 ccgacctcct cgaggccacg cttgagggtc tcttgacggg ccgcctcgcg ggattcctga
     3901 acttcctggc gcaagctgcc cacggcctcg ggaaggtcgt cgatctcgac cttcagcgtc
     3961 gccgaaatct cttcggcccg ctcggtggcg tcggtaaggt acgtcagcgc ctcgcggccc
     4021 gttatgacct cgatgcggta tagatcggcg ccatgcttac ggttcgacag gatcttgaaa
     4081 gccccgatct ccgcgctgcc ccggacgtgt gtgccgccgc agagctcgcg cgagaagcca
     4141 tcaacctcaa ccaccctgac gaggtcggag tacttctcgc cgaagagcat catcgcgccg
     4201 aggttacgtg cctcctccag ggtcgtggtg aagtacctga tcggctggtt ctctgtgatc
     4261 ttctgcagcg agatctcctg aacgcgccgg atctcctcgt ccgtcaccct cccgccgtag
     4321 cggtagtcga acctgagccg gtcaggaccc acgtagctac cggcctgaac gacttcctgc
     4381 ccgagaacgg ccctcagagc ccagtgctag atgtgcgttg cggtgtggtt cgcttctatc
     4441 tgctggcggc gcacccggtt gatggaggcg acaactgcgt ccccggtgct aaaggccccg
     4501 cgttcggttc tcgccctcag cacctggtaa tccccggcgg ggatggcgtc aacgacctcg
     4561 agttgacctt catccgaaga gacccaaccc tcgtccgcca cctgcccgcc gccagtcgcg
     4621 tagaaagggt tctccgcgag aaccacgtac acctcgtcgt cagcctccgg gacgggctct
     4681 atggccacta tcctggtctc gacctgctcg cgctcgtagc cgacgaaacg gctcctgatg
     4741 tcctcatccc tgaacgcggc gacgacccgg tcgtagccct gtacggcctc cctggcccga
     4801 tccctctgcg agtccatcgc cgcccggaac cccgcctcgt cgagcgagag ccctctgtcg
     4861 gagaggacct cccgcgttac atcgaccggg aacccgtagg tgtcatggag catgaacgcg
     4921 agttcaccgg gaaattttcc gccgtcgaag cgccgcagct cctcctccag gagatacata
     4981 ccttgatcgt agatctgcag aaagcgagcg gcctcggctt ccacgacccg tctgatatca
     5041 cccacggcat tgaatagctc ggggtaggcg tctttcatgt agttggctac ttcgcctgcc
     5101 aggctagcga gaaactgggg gctctgtgtt tgcgggttca ccttcgccag atgcaaccgc
     5161 gcctgcaggg cagccctacg aatgatcctt cgtagcacgt actcacgtcc cgtgctgccg
     5221 ggtctgaccc cgtccgcgat cagaaacgcc atcgacctgg cgtggtctcc gaggatgtac
     5281 aacgagcgat caaccgccgc cgacatcccg aactcgatct tgtaatcgtc acgaatccgg
     5341 tcgtataccg gggagtacac gtctgtctgg tagacgtagg ggacgttctg gaccgcggcg
     5401 gtagtacgtt cgagtcccat acccgtatcg atgccggtct tggccagcgg ggtcagggtg
     5461 ccgtccttgc gctgctcgta ctggttgaag acgaggttcc atatctccag gaatcgcgga
     5521 tcgccttctt ctcccccggg gccgtatctg gggtcggctt gtggatcgcc gtgcgagtac
     5581 tcttcgccgt agtcgtagta gatctccgag cacgggccac acgggcccga atcccccggt
     5641 ggtccccacc agttctcgct cttgggaaga ccgaagatct tgtgctcggg tacccccacg
     5701 ttcatccaga gttctttggc ttccagatcc tcgggggcgt cctcatcccc ctcgaagatc
     5761 gtgatccaga gcctctctgg cgggagcccc agctctaccg tgaggaactc ccaggcccag
     5821 gcgatggcct ctctcttgaa gtagtcgccg aaagagaagt tcccgagcat ctcgaagaac
     5881 gtcaggtggc tctcgtcgcc gacctcttct atgtcctggg tcctgaagca tttctgggca
     5941 ctcgtcgccc tcggggtcgg gggcctatcc tggccggtga agtaggtgat gaactgctgc
     6001 attcccgcgg tcgtaagaag aaccgtcggg tcgttgtggg ggatcaggga ggcgctgggg
     6061 taaagccggt gacccctctc cacgaagaag tccagaaagc ggcgccggat ctcgttactc
     6121 tgcacgggcg ccgtccgggg taagcgtggt ctcctcctga tcctcctcga gctgttcgag
     6181 ctccacacgc aggtcttcta tggccctcct gaggaggcgg gagacgtgca tctggctcac
     6241 gccgatgtgg tcggcgatct cggtctgggt cttgccctcg ttgaagcgga gccgcagaat
     6301 ctgctgctgc tggcccttga ggctttcgat ggcctcctgc agaagcagac ggtcctcgag
     6361 gccgtcaatc tgggaccggt catcggcctg gaggtcgatg atggtatccc cctccggctc
     6421 atcctgacta accggggcgt ccaggctggc cgtgttgtag gccctcccga gtgtcagggc
     6481 ctcggccacg ccttccacgt cagcgtccag ggtttcggcc agttcctgaa gggagggcga
     6541 acgacccgtc ctgaccgtct cgtcccttat cacctctttc gcagactggc ggagctcctg
     6601 caggccgcgc gggacgcgca tggcccatcc cttgtcgcgg aagtagcgct tgatctcccc
     6661 cagaatattg ggcgtcgcgt aggtggagaa ctctatcttc cggtccacgt cgaagcggtc
     6721 cacagccttt atgaggccga cggaggcgac ctgcaccagg tcgtccaggg gctcaccccg
     6781 gccagcgaac ttgcgtgcca ggaactcggc caatgggagc tgttcctgga ttacacgttc
     6841 cctggcaccg cgatcgcctt gcttgtggta gcggacgagc aggcgcctcg tgcgcgcttt
     6901 gtccggtctg cggtagcgcc ggctcacgaa accatctcca ccacggtgcg ccaccgcgtc
     6961 ccttcgtcac cctcgcccga gggctcctcc acctcggcca gcacaccctc gtcggcgagc
     7021 gagaatcgga aacgggtcga aacgcctgcg gaatggaagc cctcgagcgc ctctacagcc
     7081 tcctcgaggc cgccgacgcc gaccctggcc cgggccacca tccccgcgag caccaggccg
     7141 acgcaggatg tcatgtcctc gcgcggcggg agctcgagat aagcatactc tttctggcgc
     7201 tgatcttcgg tcaatccgga tccctctctc ggctctgatc tgctgcttgc ggggatgatt
     7261 ccgaaacctg tccatccggc acgggctccg tcgcggctcc cgcagcttgc gcagcgtcgg
     7321 ggtcgggcgg ggcgccacgt gcggtgtggt ggccgccgcc atgttcctcg aggtcgtgca
     7381 gaatctcctg ccgcctctgc gccgaggcct cccgtcccgc cgagacggca cgctggtagc
     7441 ggcgccggat ggtgtcgggc aggtcgcgcg ctgccctggg aaggttcttg cgtacgtcct
     7501 cattggtcgc gtagatggta ccagcaaccc cgatcacgac accggcgact acccgtcccc
     7561 agctcgccat tactcttcac cttcttgacg cttctcgccg cccaggaagg tgctgatccc
     7621 ccgcgatagg ccggccgtag ccgatgagac cttgatcacg ctccccctca cgccgcgcgt
     7681 gatgacggcc gtggtctggt ccagctcgcc ggtcatgccg gccacgtcgc ccacggcgcc
     7741 atccagctgc gagagctgac tgttgatgtt gtcaaccgtc acgtgcgtct tgcccagcag
     7801 ggggacgacc tcccccgtga cgtccttgat ggactcctcc gtagtcgaga agatgctggc
     7861 gagcctcaag aacatccagg cgagaacgaa gaaagcgacc gcgatccccg cgaatagcaa
     7921 acccttcatc aactctacga aaagttccaa accttaccgc tcctcggtac ggggcccctg
     7981 aaacccggat tataactgcg atcgcatgaa tataccgccc tcaagcggtc ccgcgggcaa
     8041 cgacgccgcc gccgacgacc ttgctaccgt ccgctgtgta gaaaacggcc gactgtcccg
     8101 gcgcaacccc catcaccggc tcgtcgaggc gcgctttcca cctcccgtcc gccatctcct
     8161 ctacggtgca cgggactggc tggctgttgt acctgacctg aacgcatgcg gcgtccccga
     8221 tctcgaggaa gcggttcagg tccgtgaccg tgacctcacg cacctccaga tcccttttgc
     8281 ggccaactac tacctggccg gaggacggcc gcacctccgt gacgtagagc ggcgtcggag
     8341 ccgagactcc aatgccacgc cgctggccga cggtgaagtc aaccacgcca ccatggcggc
     8401 cgagcacctg tccctcccgg tcgaccacgt ccccgggttc cgccgcaacc ttcttgcgca
     8461 cgaagcggcg gtagtttccg tctgggataa agcagatatc ctggctctcc ggcgtgtagg
     8521 ccacagcgag gccacgctcc ttggcgatgg cccgcacctc agtcttggtg tggtcgccga
     8581 gcgggaaaac ggtccttgcc aggagattct gtggcacggg ccagagaacg taggtctggt
     8641 ccttgctcct gtctgctgga cgcccgagcg tagggccctc ctcgaccctg gcgtagtgtc
     8701 ccgtcgcgac gtgatggtaa ccgagctgat cggcgagaaa ggcggaggcg tggaacttta
     8761 cgtgggcgtt gcacgccacg caggggttcg gcgtcctgcc ttcctgatat gagccgacga
     8821 agtcccgcat gacccgcctg ttgaagagtt ccttcaggtc gagcgtgaag tgcggcaggc
     8881 ccatcctgtg cgcggtgtcg cgggcgaaga ggacggtatc aggcgagcag cacgagcggc
     8941 tacccttctc gccatcgtgc aatcggaacg taacggtgac cacatcgtag ccggactcct
     9001 cgagcagaag ggccgtcacc gccgagtcga cgccgccgct catagcggcg accacgctcc
     9061 tgccgctacc tccacccgtg ctcatgcctt tcagcagcat ctcgcccgac gtctggttcc
     9121 agtggtcctc gaaggccttg tgcagcgcgt cgagcaccag cgtgagcgcg tgctccttac
     9181 caaggggtag cggaccgcca agggcgtcct ggatatcctc cttcgagatg cgggccgcct
     9241 cgacgatgtg cctgccctcg accagctccg agagcgccga cccggcggcc acgcaagccg
     9301 cgcagccgta gaccccgtaa cggacctcct caagcacgtt gtcagagatg ctgacggtga
     9361 agcttgcgac gtcgccgcag gcctcgtggc ctgattcgcc gcgtccggaa gggttggcga
     9421 tctcgcccgc gttccgcggc cggacgagat ggtctataac ctgtggtgtg taacgatctg
     9481 gcaccagtga cccactttca gcccgcgtag agcggggaga gttctcgtag ttgtgcgacc
     9541 gcggcaccga aggcatccag aaacccgtcg acctcctccg cggtgttgtc cttgccgacc
     9601 gtgatcctca cgaccgagaa cgcgtcctgc tcggggcggc ccatggcgag caggatcggc
     9661 gaggccttgt gcccgccgga ggagcatgcc gacccgcttc ccacctcata accggtcgcg
     9721 tccaggaaaa gtacgaggct ctcggcctcg acgccggaaa cggtgaggtg cacgttgttt
     9781 gagagccggc gcccccgggg cccgttgagg gtgacgtccg gtatcttctc caccccggac
     9841 agaatgcggt cgcggagcgc ctcttcgtgg gagatgcggt ccgctatctc ctcgcgggcg
     9901 agctcagccg ccttcccgag gcctgcaatc ccggcaacat tctgggttcc gctcctgatt
     9961 ccgttctcct gccctccccc gtacacgacg ggctcggg
//
LOCUS       DBNGVD010000063         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1831803, whole genome shotgun sequence.
ACCESSION   DBNGVD010000063 DBNGVD010000000
VERSION     DBNGVD010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1831803"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16CM_01850"
     CDS             complement(<1..670)
                     /locus_tag="AC16CM_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3138597.1"
                     /translation="MDRETPRKFATTPGTRDVLPPESTRLLDVQAEIRDRFRMFGFRD
                     VVTPALEYSEVIEEHGLRDAAYKLFDRDNQMLLLRPEMTTPIARLVAQRLAGTPEPHK
                     LSYVLPAYRRTTVGRGQSAELYQAGVEVVGSASPREDAATIALLVDVLESLGLRAPSD
                     FAVVLGQSAFYSGYLRHVAPQAATAVTAALAAKDLVAVDALAQDLPESVAEGIRGIPR
                     LVGSP"
     gene            852..1292
                     /locus_tag="AC16CM_01855"
     CDS             852..1292
                     /locus_tag="AC16CM_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138598.1"
                     /translation="MSEVGWTVVGLGDLPRVPGREDIEWLPLQHALRLGAFGTNVFVG
                     PAGGEELIGPHDETGEDGQEEMYLVLTGTVRFELAGETLDLRQGDVVAVRDRAVRRRA
                     TAMEPGSTVLVVGAPPGSLVRSTWRPEWFAGVPELGDGDRRART"
     gene            1416..2057
                     /locus_tag="AC16CM_01860"
     CDS             1416..2057
                     /locus_tag="AC16CM_01860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3138599.1"
                     /translation="MLEIWGRKNSFNVQKVLWCCEELEVPYRRHDAGGKFGGTDDDEY
                     LQRNPTGLVPTIADGDLTLWESNSIVRYLAASYGSGSLWPEDPAERALADKWMDFQLG
                     TLFPAFKDALVGLVRTAPENRDPEKIDAAVRATADVTPILDARLRNNDYVAGPSLTMG
                     DVALGSLVYRYFELAIDRPDLPALKAWHERLAERPAYQKTVMVPFTMENPPSS"
BASE COUNT          385 a          736 c          809 g          381 t
ORIGIN      
        1 tgggggaccc tacgaggcgt ggtatgcccc tgataccttc ggcgacgctc tcgggcaggt
       61 cctgcgcgag ggcgtccacg gcgacgaggt ccttggccgc gagggccgcg gttaccgcgg
      121 tggccgcctg gggggccacg tggcgcagat agcccgagta gaaggccgac tggccgagca
      181 cgacggcgaa atcggagggt gcgcggaggc ccaggctctc cagaacatcg accagcaagg
      241 cgatggtggc agcatcttcc ctggggcttg cggagccaac cacctccacg ccggcctggt
      301 agagttcggc gctctgcccg cggcccacgg tcgtgcggcg gtaggcgggc aggacatatg
      361 agagcttgtg cggctcgggg gtgccggcga ggcgctgggc cacgaggcgg gctatcgggg
      421 tggtcatctc cggcctgagc agaagcatct ggttgtccct gtcaaagagc ttgtaggccg
      481 catcgcgcag gccgtgctcc tctatcacct cggagtactc cagcgccggc gtcaccacat
      541 ccctgaaccc gaacatcctg aagcggtccc tgatctcagc ctgcacgtcg agcaggcgcg
      601 ttgactccgg gggcaacacg tctcgcgtgc ccggcgtggt tgcaaacttc ctcggcgtct
      661 cgcggtccat aatcgaggct atcttaaccg atgagcatgc cctttgacgc ctggtccagg
      721 caagagttcg ggcgggcgtt acggcggggg aacccgtagc gcccggcctg ctatcttgct
      781 accatcaccg cttaccgagg agagaggagc tgtcgggtgg gcttccgccg gggtaccgcg
      841 agaatggcgc gatgagcgag gtcggctgga ccgtggtcgg gctcggcgat cttccgcgcg
      901 tgccaggccg tgaggacatc gagtggctgc cgctgcagca cgcgcttcgg cttggcgctt
      961 tcggcaccaa cgtgttcgtc ggaccggcgg gaggcgaaga gctgatcggc ccgcacgacg
     1021 agacgggcga ggacggccag gaagagatgt acctggtgct gaccggcacc gtgcgcttcg
     1081 agcttgcagg cgagacgcta gatttacgtc agggagacgt cgtcgcggtg cgcgaccgtg
     1141 cggtgcggcg cagggctacc gcgatggagc cggggagcac cgtgctggtg gtcggggcac
     1201 cgccggggag cctcgtgcgc tctacgtggc gtcctgaatg gttcgccggc gtccctgagt
     1261 tgggagacgg cgacagacgc gcccggacct gagcatgttc tcgtcggagc gtcgcaggga
     1321 tgctcgtgga ccacgttcat gtttcttacc atcgacctgt cggactacgg gcgtgatggg
     1381 gcgcccccgg gagcaacgcg ggaaaggatg atctgatgct cgaaatctgg ggcaggaaga
     1441 actcgttcaa cgtgcagaag gtgttgtggt gctgcgagga gctcgaggtt ccgtacaggc
     1501 gtcacgatgc cggcggcaag ttcgggggca ccgatgatga cgagtacctg cagcgaaacc
     1561 cgaccggcct cgtgccgact atcgccgatg gggatctcac gctctgggaa tcaaactcta
     1621 tcgtgcgcta cctcgcggca agttacgggt ccggcagcct gtggcccgaa gaccccgccg
     1681 agcgggcgct cgccgacaag tggatggact tccagctagg caccctcttc cccgccttca
     1741 aggacgccct ggtcggcctg gtgcgtaccg cccctgagaa tagagacccc gagaagatag
     1801 acgctgccgt ccgggccacg gccgacgtga cacccatcct ggacgcccga ctccggaaca
     1861 acgactacgt tgcaggcccc tccctcacca tgggagatgt ggccctcggc tcgctcgtct
     1921 accgctattt cgagctggcg atagacaggc cggacctgcc cgcccttaag gcctggcacg
     1981 agcgcctagc ggaacgtccc gcctaccaga agaccgtcat ggtccctttc acaatggaga
     2041 atccaccttc cagctagggt gcgcccccag cgccgccagc aagaactcgg atcggtttcc
     2101 tgagccagga gaccccgtac cgtcgggcac cggcggtgcg accgcaaccg gtcagggcgt
     2161 ggatctctaa gcagcgtaga cactccagag gcgagcaagc tgctccgagt tcgccgcgcc
     2221 gaaggcctgg ggagggccta ggctttccgg ccccggccct cccggatgtg tggtccgtcg
     2281 cgttggcccc gggctcaggg cgccgggaag g
//
LOCUS       DBNGVD010000064         8134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3653978, whole genome shotgun sequence.
ACCESSION   DBNGVD010000064 DBNGVD010000000
VERSION     DBNGVD010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3653978"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="AC16CM_01865"
     CDS             complement(<1..1213)
                     /locus_tag="AC16CM_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3138600.1"
                     /translation="MAESTRGPRPLEGVRVVEMGSLLAGPFCGQLLADFGAEVIKVEP
                     PGKGDPMRVWGRHRKEGRTLWWPIIARNKKSITLNLREEQGQELARRLIASADVVVEN
                     FRPGTLERWGLGYEDLREINPGLVMVRVSGYGQTGPYRDQAGFGSIGEAMGGIRHVTG
                     FPDRPPPRVGISLGDSLAATFGALGAVTALYNRETNGGRGQMVDVAIYEAVLALMEST
                     IPEYALAGHARGRTGAILPFVAPSNTYPTSDGDYVVIGANADTVFGRFAGAVGHPEWA
                     ESEEYATHHARGENQQELDDKISAWTGQRTAAEVLEAMKEAGVPAGKLFTAEDMLSDE
                     QYAARGSIVEVEDPDIGPFPMQNVVPRLSETPGEVRWTGPKLGQHNEEVYEKVLGMDE
                     EDLTALRERGII"
     gene            complement(1206..1895)
                     /locus_tag="AC16CM_01870"
     CDS             complement(1206..1895)
                     /locus_tag="AC16CM_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3138601.1"
                     /translation="MGRVLRDAGHESGSVGILERLRALILTGEYGPDERLVEEQLAER
                     LGVSRTPVRQALTMLEAEGLVEIAPNRGATVCSFSTEDVWDIYDLRAVLEGHAARRAA
                     GRIVDGELRRLRELTGEMEGLSGRFEDHEEEIRALVGLNQEFHGIIVEASRNRRLQRL
                     INRTVEIPLMFKAFFWYTPHERIISNHYHRQILEALENGDADRAEIIMREHVYEGRDF
                     VIRALKEDMNG"
     gene            complement(2079..2672)
                     /gene="lepB"
                     /locus_tag="AC16CM_01875"
     CDS             complement(2079..2672)
                     /gene="lepB"
                     /locus_tag="AC16CM_01875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3138602.1"
                     /translation="MSEVPPRGSGERPPENSWRRQLLEYVLTLAFAFALVFGFIKPAV
                     ADSYRIPSSSMEPTLHGCAGCNNDRVMANKFIYRFSEPERGQIVVFRSVEDGDTLLIK
                     RVVGVPGDKIQLRGGTLYLNGEPQDEPYVHTNPCNRHMPRTCAFGPVTVPRGHVFVMG
                     DNRTNSRDSRFFGAVPEDNVEGEAFLRYWPPGRIGLL"
     gene            complement(2731..4683)
                     /locus_tag="AC16CM_01880"
     CDS             complement(2731..4683)
                     /locus_tag="AC16CM_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138603.1"
                     /translation="MTFGSLLIANRGEIAVRLIRACRELGIESIAVYSEADERAMHRR
                     LADEASPIGPAPASESYLNTGRLVEAIERSGAEAVHPGYGFLSESAGFARAVEAAGAV
                     WVGPTPEAMDAVGHKVRAKELARRADVPTIPGYEGEEEGEERLAEEARRMGFPVLVKA
                     SAGGGGRGMRSVAREDDFIEAVRGARREAEAAFGDASVFLEKLVEAPRHVEVQVIGDR
                     HGHVIHLFERECSIQRRHQKVVEEAPSPALDPALRDSICEAAVRFARGAGYTNAGTVE
                     FLLDGDGFYFLEMNARLQVEHPVTELVTGLDLVHLQLAVASGEPLPLAQDDVTLRGSA
                     IEARIYAEDEAGMPSGGLLETFVTPEGAGVRNDAGFEAGDEIPLDYDAMLAKLVVSAP
                     DRPAAVRRLRRALTDYEILGPSSNLPLLRRIAAHPTFERGETTTRFLEGHHLTEPSAT
                     ESVPREALVLACAAEISGIVSSPDPFAAGPWRLLGATTLRYQDQRVGVERIGSSRFRI
                     TAEGGESIVEVLHRGEGRIRALVDEEPVEAGIVVKADGILVSLRGEAYVLRKPPPLAL
                     DAAGRGADTAASLTAPMPGTVVKVLVEKGQEVEEGQLLLVLEAMKMEQPVSAPHAGVV
                     QALPFEEGSLVPGGAVLVEVEEKSSK"
     gene            complement(4680..5468)
                     /locus_tag="AC16CM_01885"
     CDS             complement(4680..5468)
                     /locus_tag="AC16CM_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3138604.1"
                     /translation="MPYQYLNRADDGVVTTVTLARPDLHNALNVGLIEELTSCFEEIS
                     DDDGVRVVVLTGEGRSFCAGADIGYMRETAGFTYEDNLEDARRLAAMYWTIDECPKPL
                     VARVHGAALGGGSGLVAVSDVVVASEEARFAFTEVRLGIAPATIVPFVVRKIGASHAR
                     SLFITGERFGAKKAREIGLVHNLAPRDGLDAVVEEKVGEILQGGPVAQATVKALLRQL
                     ETTEPMEAPGLTSRVISELRTGEEGQEGLAAFLEKREPDWRASR"
     gene            complement(5611..7218)
                     /locus_tag="AC16CM_01890"
     CDS             complement(5611..7218)
                     /locus_tag="AC16CM_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3138605.1"
                     /translation="MSRLGTLVNTSSEAFAENVAGFARLVAELRERSAEARRGGGEKA
                     QKRHRERGKMPVRDRVDGLLDPGTAFLELSSLAAFGMYDGRVPGAGIITGIGRVSGRE
                     VMVVANDATVKGGTYYPVTVKKHLRAQEIAEQNHLPCIYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFYNQARMSAKGIPQVAAVMGSCTAGGAYVPAMSDEVVIVEGEGSIFLGGPPL
                     VKAATGEEVSAEELGGADVHTRRSGVADHFARDDEHALAIVRSIVENLNHEKRVPWTV
                     EEPEEPLYDPAELYGVVPSDYRQGYDVREVIARTVDGSRLHEFKPLYGETLVCGFARI
                     MGHPVGILANNGILFSESALKGAHFIELCCSRGIPLVFLQNITGFMVGREYEAGGIAK
                     DGAKLVMAVANANVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGQQA
                     ANVLLTVQEQNLKREGREMTDEEREDFKDPILEKYEEEGNPYHSTARIWDDGVIDPVD
                     TRMVLALGLSAAANAPLEETTFGVFRM"
     gene            7374..7997
                     /locus_tag="AC16CM_01895"
     CDS             7374..7997
                     /locus_tag="AC16CM_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3138606.1"
                     /translation="MTKMESTHQRILWRGLEIVSERGLGGVSLGGLADRAGMSKSGLF
                     AHFRSKEELQVELLGAAEAALKREVVDPIVEVPEGLPKLRALVGRWLGWAARSGLPGG
                     CPLYAAAFELDDAEEGPVRDFLMQSKGEWSRMLEGLVKEAVALGHLEEDLDAVQFVWQ
                     LEGIYLSHHVSQRLMRDPEADARALAAFDGLVASSLSGQTPRPEPGS"
BASE COUNT         1348 a         2762 c         2543 g         1481 t
ORIGIN      
        1 agatgatgcc ccgctcccgc agggccgtga ggtcctcttc gtccatcccg agcaccttct
       61 cgtagacctc ttcgttgtgc tggcccagct tcggaccggt ccagcggacc tcgcccggcg
      121 tctcggaaag ccgggggacc acgttctgca tcggaaaagg ccctatatcc gggtcttcga
      181 cctcgactat gctacccctg gcagcgtact gctcgtcgga gagcatgtct tccgctgtga
      241 acagcttgcc ggccgggacg cccgcctcct tcatggcctc gagcacctct gcggccgtcc
      301 gctggccggt ccaggcggag atcttgtcat ccagttcctg ctggttctca ccgcgggcgt
      361 ggtgcgtggc atactcctcg ctctcggccc actcgggatg tcctacggcc ccggcgaagc
      421 ggccgaacac cgtgtcggcg tttgccccta tcaccacgta gtcgccatcg cttgtggggt
      481 aggtgttgga cggtgccacg aagggcagga tggccccggt gcggcccctg gcgtgcccgg
      541 cgagcgcgta ctccgggatg gtgctctcca tgagagcgag cacggcctcg tagatggcca
      601 cgtcaaccat ctggcccctg ccgccgttcg tctcgcggtt gtagagggcg gtcacggcgc
      661 caagggctcc gaaggtcgcg gcgagcgaat cgcccaggct gatccccacc cgcggtggcg
      721 gacggtcggg gaacccggtc acgtgcctga tgccacccat cgcctccccg atggacccga
      781 agcctgcctg atcccggtac ggccccgtct gcccgtaccc ggagacgcga accatgacga
      841 ggccggggtt gatctccctg aggtcctcgt acccgaggcc ccaacgctcc agcgtccccg
      901 gcctgaagtt ctcgacgacc acgtccgcgc tggcgatgag ccgccgggcc aactcctgcc
      961 cctgctcctc ccgcaagttc agcgtgatgg acttcttgtt gcgggcgatg atgggccacc
     1021 agagggtcct cccctcttta cgatgcctgc cccagacccg catggggtcc cctttgcccg
     1081 ggggctccac cttgatgacc tcggcaccga agtcggcgag tagctggccg cagaaagggc
     1141 cggcgagcaa gctgcccatc tcgaccacac gcacgccttc taggggacgc ggtcccctgg
     1201 tactctcagc cattcatatc ctccttcaaa gcccttatca cgaaatcgcg cccctcgtag
     1261 acgtgctcgc gcatgatgat ctccgccctg tccgcgtcac cgttctcaag ggcctccagg
     1321 atctggcggt ggtaatggtt ggagatgatc cgctcgtgcg gcgtgtacca gaagaaagcc
     1381 ttgaacatga gcggtatctc gaccgtgcgg ttgatgagcc gctgcaatcg cctgttgcgg
     1441 ctcgcctcga ctatgatgcc gtgaaactcc tggttgagcc cgacgagagc ccgtatctct
     1501 tcctcatggt cctcgaaccg cccggagaga ccctccatct cccccgtgag ctcccgtaac
     1561 ctccgcagct caccatcgac tatccgacca gccgcccgcc tcgcggcatg cccctccagc
     1621 acggcccgca ggtcgtagat gtcccatacg tcctcggtgg agaaggaaca taccgtggcc
     1681 ccacggtttg gcgctatctc caccaacccc tcagcctcca acatggtcag agcctgccgc
     1741 accggcgttc tactcacccc gagcctctcc gcgagctgct cctccaccag gcgctcgtcg
     1801 ggtccgtact cgcccgtcag gatcaaagcc cgcagccgct ccagaatccc gaccgacccc
     1861 gattcgtgcc ccgcatcgcg caaaacccga cccactaagg cccccgatcg taacccccgg
     1921 agaactggtt gtatacaatc ttaggcgcac gcaaatcttg tgtcaagcat tccaaatccc
     1981 ggcaggccca tgcggctcgc atagggccac ggggaatttc gagacggctt ggatgagggt
     2041 ctcgcgggga gaatcgttgg ctgctacggg gcggtcctct agagcagacc gattctgccg
     2101 ggtggccagt accgcaagaa ggcctcacct tccacgttgt cctccggcac ggccccgaag
     2161 aaccgcgagt cacgggagtt ggtcctgttg tcacccatca cgaaaacgtg tccccggggc
     2221 accgtgacgg ggccgaaggc gcaggtcctg ggcatgtgcc tgttgcaggg gttcgtgtgc
     2281 acatagggtt cgtcctgcgg ctcgccattc aagtagaggg tgccgccgcg gagttggatc
     2341 ttatctcccg ggacaccgac cacgcgcttg atgaggaggg tatctccgtc ctctacgctc
     2401 ctgaagacca cgatctggcc cctctcgggc tcggaaaagc ggtagatgaa cttgttggcc
     2461 atcacccgat cgttgttgca acccgcgcag ccgtggaggg tgggctccat gctggacgag
     2521 gggatgcggt aggaatccgc tacggcgggt tttatgaagc cgaaaacgag cgcaaaggcg
     2581 aaggccagcg tgagtacgta ctcgagaagc tggcgccgcc agctgttttc tgggggtctt
     2641 tcgccactgc cgcgcggggg cacttcactc atacccggga tactagcatg cattatcgag
     2701 ttttcgtctg gtagctgtcc cgcctctttc ctacttcgag ctcttctctt ccacctccac
     2761 gagcacggcg cctccgggaa cgagggaacc ctcctcgaaa ggcagcgctt gaaccacacc
     2821 ggcgtgcggc gccgacaccg gctgctccat cttcatcgcc tcgaggacga gcaggagctg
     2881 cccctcctcg acttcctgtc ctttttcgac cagcaccttc acgaccgtac ccggcattgg
     2941 cgccgtcagg ctcgccgctg tatccgcccc ccgaccggcc gcgtccaggg ccagcggcgg
     3001 gggcttgcgg agcacatagg cctccccgcg caaggagaca aggatcccat ccgccttcac
     3061 cacgatcccg gcttcgaccg gctcttcatc gacaagagcc ctgatgcgac cttctccgcg
     3121 atggagcacc tcgacgatgg attccccgcc ctccgccgtg atgcggaagc gtgacgatcc
     3181 tatccgttcc acacccacac gctggtcctg gtatctcagg gtggttgcgc cgagcaggcg
     3241 ccagggtcct gcggcgaaag ggtcgggaga gctcacgata ccggagatct ctgccgcgca
     3301 ggcgagtacc agggcttcgc gcggtacaga ctctgtagca gagggctccg tgagatggtg
     3361 cccttcgaga aaacgggtcg tagtttcgcc ccgctcgaac gtcgggtggg ccgctatacg
     3421 cctcaggagc ggtaggttgg aggagggacc gaggatctcg tagtccgtga gggcccggcg
     3481 caggcggcga acggcggccg ggcggtcggg ggcggagacg accaacttgg ccaacatggc
     3541 gtcgtagtcg agcggtatct catcgcccgc ctcgaacccg gcgtcgtttc ggacgccggc
     3601 accctcgggc gtgacgaagg tctcgagtaa ccctcccgag ggcatgcccg cttcgtcctc
     3661 ggcgtagatg cgcgcctcga tggcgctacc ccgcagggtt acgtcgtcct gtgccagggg
     3721 aaggggctcc cccgaggcga cggcgagctg gaggtggacg aggtcgaggc cggtaacgag
     3781 ctcggtgacg gggtgctcta cctgaagcct ggcgttcatc tccaggaagt agaagccgtc
     3841 tccgtctagc aggaactcca ccgtacccgc gttggtgtag ccggccccgc gggcgaagcg
     3901 gacggcggcc tcgcaaatcg agtcgcgaag tgcggggtcg agggccggag aaggggcctc
     3961 ctccaccacc ttctggtgac ggcgttggat ggaacactcc cgctcgaaga ggtggatcac
     4021 gtgtccgtgt ctgtccccga tcacctgcac ctcgacgtga cgcggggcct cgaccagctt
     4081 ctccaggaac accgacgcat caccgaaggc ggcctcggcc tccctacgtg cacccctgac
     4141 cgcctcgatg aagtcgtcct cgcgcgcgac ggaccgcatc ccgcgtcctc ctccgccggc
     4201 gctggccttg accaggacgg gaaagcccat gcggcgcgct tcctcggcga gccgttcctc
     4261 gccttcctcc tcgccttcgt agccggggat cgtcggcacg tccgccctgc gcgccagctc
     4321 cttggccctg accttgtggc cgacggcgtc catcgcctcc ggtgtggggc ccacccagac
     4381 cgcgccggcc gcttcgacgg ccctggcgaa cccggcgctc tccgagagaa agccgtaacc
     4441 gggatggacc gcttcggccc ccgacctctc gatggcctcc acgagccgcc cggtgttcag
     4501 gtagctctct gaggccggag ctggaccgat gggggacgcc tcgtcggcga ggcgacggtg
     4561 catcgcgcgc tcgtctgctt ccgagtagac ggcgatgctc tctattccca gctctcggca
     4621 ggcacggatc aggcgcaccg ctatctcgcc gcggttggcg atgaggaggc tcccgaacgt
     4681 caccgggacg ctctccagtc cggctctcgc ttctcgagga aggccgccaa cccctcctgg
     4741 ccctcctccc cggtgcgcag ctcggagata acgcgcgagg tcagcccagg cgcctccatc
     4801 ggctcagtcg tctcaagctg gcggagcaga gccttcaccg ttgcctgggc gaccggaccg
     4861 ccctgcagga tctcgcctac cttctcctcg accaccgcgt cgagcccatc ccgcggcgca
     4921 aggttgtgga ccaggccgat ctcccgcgcc tttttcgccc cgaagcgttc acccgtgatg
     4981 aacagcgatc tggcgtggga ggcccctatc ttgcgcacca cgaacgggac gatcgtggcc
     5041 ggcgcgatcc caagccggac ctcggtgaag gcaaaccggg cctcctcact ggcgacgacg
     5101 acatcggaca cggcgacgag gccagaccca cctcccagcg cagccccgtg caccctggca
     5161 accaggggtt ttgggcactc gtcgatggtc cagtacatgg cggccagccg tctggcgtcc
     5221 tccaggttat cctcgtaggt gaaccccgcg gtctctcgca tgtacccgat gtctgcgccg
     5281 gcgcagaacg accgtccctc cccggtaagc accaccacac gcacaccgtc gtcatcggag
     5341 atctcctcga agcagctggt cagctcctcg atcaggccta cgttcaacgc gttgtgcaaa
     5401 tcgggacggg ccagggttac ggtcgtcacc acgccatcat cggccctgtt caggtattga
     5461 tagggcataa ggtgtcaggc tacaggtttc agggtgtgcg cagatgctgc cgcaaggact
     5521 cgatcgcggt ggggatagtt gtttgccggg gtatgtgtca cacatgctcc ttcctgaagg
     5581 ctgatggctg tagcctgctg cctcgaaaac ctacatcctg aagacgccga atgtagtctc
     5641 ctccagcggc gcgttcgcgg ccgccgagag gccgagggcg aggaccattc tcgtgtccac
     5701 agggtctatg acgccgtcgt cccagatcct tgcggtcgaa tggtaggggt tgccttcttc
     5761 ctcgtatttt tcgaggatgg ggtctttgaa gtcttcgcgc tcctcgtcgg tcatctcccg
     5821 gccctcgcgc ttcaggttct gctcctgcac ggtcaggagc acgttggctg cctgttgacc
     5881 ccccatgacg ctgatacggg cgttgggcca catccagagg aagcgcggag agtaggcccg
     5941 gccgcacatc ccgtagttgc cggccccgaa ggagccgccg atgatcacgg tgaacttggg
     6001 gacgttggcg ttggccacgg ccatgaccag cttggcccca tccttggcga ttccgccggc
     6061 ctcgtattct cggccaacca tgaagccggt gatgttctgc aagaagacga gcgggatgcc
     6121 acgagagcag cacaattcta tgaagtgggc gcccttgagc gcgctctcgg agaaaagtat
     6181 gccgttgttg gccaggattc ctacgggatg gcccatgatg cgggcgaacc cgcacacgag
     6241 cgtctcgccg taaagcggct tgaactcgtg aaggcggctt ccatccacgg tcctggcgat
     6301 aacctccctc acgtcgtagc cctgcctgta gtcgcttggg acgacgccgt agagctccgc
     6361 cgggtcgtag agaggctcct ccggctcctc cacggtccag ggcacccgct tctcgtggtt
     6421 caggttctcc acgatggagc gcacgatggc gagggcgtgc tcgtcgtccc tggcgaagtg
     6481 gtcagccacg ccggagcggc gggtgtggac gtcggcgccg cctagctctt cagccgagac
     6541 ctcctcgccg gtcgccgcct tcaccagggg agggccgccg aggaagatcg agccctcccc
     6601 ctcgactatg acgacctcgt cgctcatggc gggcacgtag gccccgcccg ccgtgcagct
     6661 tcccatcacg gcggcgacct ggggaatgcc cttcgcgctc atcctggcct ggttgtagaa
     6721 gatgcggccg aaatgctcgc ggtcggggaa cacctcgtcc tgaagcggca gaaaggcacc
     6781 cccggaatca acgagataga tgcaaggcag gtggttctgc tccgcgatct cctgcgcccg
     6841 caaatgcttc ttgaccgtca ccggatagta ggtgccaccc ttcacggtcg catcgttggc
     6901 gaccaccata acctcgcgcc cagagacgcg tcctataccc gtgattatcc ccgcaccggg
     6961 aacacgcccg tcgtacatcc cgaaagcagc cagggatgag agctcgagaa aggccgtgcc
     7021 agggtcgagc aaaccatcga cccgatccct cacgggcatc ttgccacgct ccctatgacg
     7081 cttttgcgcc ttctcccccc cacctcgccg ggcctcggca gagcgttcgc gcaattccgc
     7141 gaccaacctg gcgaagcccg ccacgttctc ggcgaaggcc tcgctgctgg tgtttaccaa
     7201 cgttcctagc ctgctcatgc cctcccgtcc gcctggccca gactacatgg catgttagcg
     7261 atggccagct tcacgtgcca gggtacacac ccgcaacccc acgcagaccg cgtaacccgg
     7321 gccgaaatga tatctagttg aaatagcacg accggtcgtg ctattatccg ggcatgacaa
     7381 agatggagag cacacaccag cgaatactgt ggcgagggtt ggagatcgta agcgagcgtg
     7441 ggctcggggg ggtgagcctt ggtgggctgg cggatcgggc aggcatgtcc aagagtgggt
     7501 tgttcgcgca cttccggtcc aaggaggagt tgcaggtaga gctgcttggc gcggccgaag
     7561 cggctttgaa gcgggaggtc gtggatccaa tcgtggaggt accagaaggg ttaccgaagt
     7621 tgagggcatt ggtagggcgt tggctaggat gggcggcacg atccgggcta ccgggcggat
     7681 gtccccttta cgctgcggcc ttcgagctcg acgacgctga ggagggcccc gtgcgggact
     7741 tcctgatgca gagcaaaggc gagtggtcgc ggatgctgga ggggctggtg aaggaggccg
     7801 tggcgctggg acacctggag gaggatctgg acgctgtcca gttcgtctgg caactagagg
     7861 ggatctacct gtcccaccac gtgtcccagc ggctcatgcg ggaccccgaa gcggacgcgc
     7921 gtgcccttgc cgccttcgac gggcttgtcg cctcctcgct ctcgggtcag acgccgcggc
     7981 cggagccggg atcatgacca ggaaaagcct ggcttctgag catcgtagct ggggcacggt
     8041 cctgcggcgg tgctggccaa cgctcgttgg tgtcctggcc ggcacgctct cgctcgtgag
     8101 cgatggcgtg gacggttccc cggtaacggc cgag
//
LOCUS       DBNGVD010000065         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4019248, whole genome shotgun sequence.
ACCESSION   DBNGVD010000065 DBNGVD010000000
VERSION     DBNGVD010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4019248"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2228)
                     /locus_tag="AC16CM_01900"
     CDS             complement(189..2228)
                     /locus_tag="AC16CM_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR3138607.1"
                     /translation="MGIAGDIALILVAALLGGIVARRLGLPLILGYIAAGVLVGPNTI
                     GPSVANVHQIELLAEIGVALLLFTIGLHFSLRELAPVRRIALIGTPIQMVLTIAFGYV
                     LGRVLLDLGWVESVWLGALISLSSTAVVLKTLGEQGVLGTLSARVMIGMLIVQDLAIV
                     PLLTVLPVLKNVGEGLPELGFALLKAAAFIGAMLFLGSRVLPLILARLAAWGSRELFL
                     VSVVAIGLGVGYATYLVGLSFAFGAFVAGMVLSESDYSHQALAEIEPLRDVFAMLFFV
                     SVGLLIDPLFLWEEAGTIALFVVLVLLAKGLIFGGLSKAFGYANIAPFAVGLGLFQVG
                     EFSFLLAREGISENAISQETYSLVLATAAITMAMTPLAARLTPILYGRYRRRFPREPL
                     RTFNLPQGGLHSHAILAGYGRVGSFVARLLDRLEKPFVIIEANPGRADAAREAGFPTV
                     YGDAAAGPVLEAAGAAEARLVVLTIPDAIAARLAIERAQSLAPRADILVRAESIGQLE
                     DLGRLGVYEAVQPELEAGLELARQALARFGVGAEEAHNFADMVRRELYAPISTEEATD
                     DTGEGFLLRLRQASRAIEVEWVRLPEEVELEGGDPGNGGRPASGLLGRTIGELAVRTE
                     TGASVLAVVRGDEVISNPGASLRLEPGDAVGILGTPEQRADFRAIAGTLAADSRG"
     gene            complement(2312..>3588)
                     /locus_tag="AC16CM_01905"
     CDS             complement(2312..>3588)
                     /locus_tag="AC16CM_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3138608.1"
                     /translation="AGRRAAVGRWTGAAVVFAWALLGLLWAQVILLPNALLPPTSVAE
                     LLGEVPSSTQPERFILMLFKETSLLLTAFSLLGVLLAALVYRGGARRSSLLAAGLCAV
                     AAAASLAPAAQAWKVASAEDVSLSLPGYFAGPAFAGEHPPETVTYASPGGEALEADVW
                     RPPDEGAATAADDSGDRPAVIVVHGGGWRSGERGDFPLWNAWFANQGYVVFDIDYRLS
                     PPPTWKQAPADVGCAVGWVKENAARYHVDPEQVVLMGRSAGAHLALLSAYTRHSVPEP
                     GCGARYPHDTGVAAVVAFYPPTDLARLSSQGYLVGMDRFMGGTPKSVPGRYRDLSPIS
                     HVDPHDPPTFLAHGGDDQIVPPEQSGLLAGRLREAGVPHQLVELPWANHTFDFRWGGW
                     GSQITRSTLQDFLEVHLAAPRGARDHPSPQDQ"
BASE COUNT          639 a         1223 c         1207 g          519 t
ORIGIN      
        1 ttcgaccgga aaatatgacc gtatcacaac cccacaactc acccagtaga caagccccgt
       61 cgccccggca ggacggggaa cctaatcttc aatcagcacc gggtgagagc ctccgcgtcg
      121 cgaccggaaa tctgggatcg aacagcatca ggtatctctc ctgaagcctt ctccctgtgt
      181 tctgcggttt acccccgaga atctgcggcg agcgtcccgg ctatcgcgcg gaaatccgcc
      241 ctttgctccg gtgtgcctag gatgcccacc gcgtcgccgg gctcgagccg caggctggcg
      301 cctgggttcg agatcacctc gtcccccctg acgaccgcca gcacggacgc cccggtctcg
      361 gtcctgaccg cgagctcccc gatcgtgcgt cccaggaggc cgcttgccgg gcgaccaccg
      421 ttcccggggt ctcctccctc gagctccacc tcttcgggaa ggcgcaccca ctcgacttcg
      481 atggcccgcg aggcctgtcg cagcctgagg aggaacccct cgcccgtatc gtcggtggcc
      541 tcttccgtgg agatgggggc atacagctcg cgcctgacca tatccgcgaa attatgggcc
      601 tcctcggccc ccaccccgaa gcgggccagg gcctggcggg ccagctccag gccggcctcg
      661 agctccggct gcaccgcctc gtagacgccg aggcgaccga gatcctctag ctgaccgatg
      721 ctctcggccc gaaccaggat gtctgcccgg ggggccaatg attgggcccg ttcgatggcg
      781 agcctggcgg ctatagcgtc ggggatggtg agcactacca ggcgggcctc ggctgctccg
      841 gcggcctcca ggaccgggcc tgctgcggcg tctccgtaga ccgtgggaaa acccgcctcc
      901 ctggccgcat ccgcgcgtcc ggggttggcc tctatgatga cgaagggctt ttccagcctg
      961 tccagcagcc tggcgacgaa ggagcccacc cggccgtacc cggcgaggat cgcgtgtgaa
     1021 tgcagcccgc cttgcggcag gttgaaggta cgcaacggct ccctcgggaa ccttctccgg
     1081 taccttccgt agaggatggg tgtaagcctg gccgccagcg gggtcatggc catcgtgatc
     1141 gccgcggtgg cgagcacgag cgagtacgtt tcctgcgaga tcgcgttctc gctgatcccc
     1201 tccctggcca gaaggaagga gaactccccg acctggaaga gtcccaggcc gacggcgaag
     1261 ggcgcgatgt tcgcgtagcc gaaggccttt gagaggccgc cgaagatcag gcccttcgcg
     1321 agcaggacca gcaccacgaa gagagcgatg gtcccagcct cttcccagag gaacaagggg
     1381 tcgatcagta gccccaccga gacgaagaag agcatggcga agacgtccct caggggctcg
     1441 atctcggcga gggcctggtg gctgtagtcc gactcggaga gaaccatccc ggccacgaaa
     1501 gccccgaagg cgaaagacaa cccgacgagg taggtcgcgt acccgacccc gaggcctatt
     1561 gccaccacgg agaccaggaa cagctcgcgc gagccccagg cggccaggcg cgcgaggatg
     1621 agtggcaaga ccctcgagcc caggaagagc atcgccccga tgaacgccgc tgccttgagc
     1681 agggcgaacc ctagctcggg gaggccctcg cccacgttct ttagcacagg caagaccgtc
     1741 aggagtggga cgatggcgag gtcctgcact atgagcatgc cgatcatgac ccgcgccgag
     1801 agggtgccaa ggaccccctg ctcgcccagg gtcttcagta ccaccgccgt gctcgagagc
     1861 gagatcaggg ctccaagcca cacggactcg acccacccga ggtccaggag tacccgcccg
     1921 aggacgtacc cgaaggcgat ggtcaggacc atctggatgg gggtgccgat cagggcgatg
     1981 cggcgcacgg gtgcaagctc ccgcagggag aagtgcaggc cgattgtaaa caggagcagc
     2041 gccaccccga tctccgccag aagctctatc tggtgcacgt tcgccaccga ggggcctatg
     2101 gtgttcggcc cgacgagcac accagcggcg atgtagccaa ggatcagggg cagcccgagc
     2161 ctgcgggcca cgatccctcc caggagggcc gccaccagaa tcagggctat gtcgcctgct
     2221 attcccatga ggttctcact ctaccatcag cctaatacgg caccgtgctg cgaccgcgct
     2281 tcccgacagg gaatacctag ccgtgctctt cttactggtc ttgcggagaa ggatggtccc
     2341 gcgctcccct cggggccgcc aggtggacct ccagaaagtc ttgtagggtg gagcgcgtta
     2401 tctgggaacc ccacccgccc cagcggaagt cgaaggtgtg gtttgcccag ggcagctcga
     2461 cgagctggtg gggtacgcct gcttccctca gccgccccgc gagcagcccg gattgttccg
     2521 ggggcactat ctggtcgtcg cccccgtggg ccaggaaggt cggcggatcg tgggggtcga
     2581 cgtgggagat gggggagaga tcccggtagc gtccgggcac ggatttcggg gtgccgccca
     2641 tgaaccggtc catgccgacc aggtagccct gggaggagag gcgggcgagg tccgtcggtg
     2701 ggtagaacgc caccacagcg gccaccccgg tgtcgtgcgg gtaacgggcg ccgcaaccgg
     2761 gctccgggac ggagtggcgc gtgtaggcgc taagaagcgc caggtgagca ccggcggagc
     2821 ggcccatcag gaccacctgc tctgggtcga cgtggtatct ggctgcgttc tccttgaccc
     2881 accccacggc gcatcccacg tccgcggggg cttgcttcca ggtggggggc ggcgagagcc
     2941 ggtagtcgat gtcgaagacc acgtagccct ggttcgcgaa ccaggcgttc cagaggggaa
     3001 agtcgccccg ctcccccgaa cgccaacctc caccgtgcac cacgatcacc gcaggccggt
     3061 ctcccgagtc gtctgccgcc gtggcggcgc cctcgtctgg aggccgccag acatcggcct
     3121 cgagcgcctc gcccccaggg ctggcgtagg tgacggtctc gggcgggtgc tccccggcga
     3181 aggcgggccc cgcgaagtac ccggggagcg agagcgatac atcctcggcg gaggcgacct
     3241 tccacgcctg cgccgccggc gccagagagg cggccgcagc caccgcgcac aggccggcgg
     3301 cgaggagcga gctcctgcgg gctccccccc ggtacacgag cgcggctaga agcacgccta
     3361 ggagggagaa ggcggtcagg aggaggctcg tctccttgaa gagcatcaag ataaagcgct
     3421 cgggttgggt tgaggacgga acctccccga gcaactcggc caccgaggtg ggggggagaa
     3481 gggcgttggg aagcaggatc acctgcgccc agagcagccc gagcagagcc cacgcgaaaa
     3541 cgaccgcggc gccggtccac ctgcccacgg ccgcacgtcg gcccgccc
//
LOCUS       DBNGVD010000066         3234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_193364, whole genome shotgun sequence.
ACCESSION   DBNGVD010000066 DBNGVD010000000
VERSION     DBNGVD010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_193364"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="AC16CM_01910"
     CDS             complement(<1..831)
                     /locus_tag="AC16CM_01910"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3138609.1"
                     /translation="METTDLRHRSRTILEGRDRAPARSFLKGMGFSDEDLSKPIVGVA
                     HSWIETMPCNFNHRHLAEKVKDGIREAGGTPMELNTIAISDGITMGTQGMKTSLVSRE
                     VIADSIELVGRGHMFDALVTIVGCDKTLPAAAMAHARLGIPGTIVYSGSIAPGRFKGH
                     DVTIQDVFEGVGANAAGKMSDEDLLDLEEHACPGAGACGGQYTANTMAMVLEFLGLSP
                     MGSAGPPALDGHKDVVCTQAGQLVMEMLERGLTPDRILTREAFDNGIAAGAATGGSTN
                     I"
     gene            994..1506
                     /locus_tag="AC16CM_01915"
     CDS             994..1506
                     /locus_tag="AC16CM_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3138610.1"
                     /translation="MQTSGRAGRVIAPGTRIGHVHLKVADIERALGFYRDVLGFEITQ
                     WYGEDAVFLSAGGYHHHIGLNTWMSRNADPAPTRSAGLFHLAILYTERRDLAQALRWL
                     VDSGYPIEGASDHGVSEALYLSDPDGNGVELYWDRPEEEWPRTPEGGLAMFTRPLDVD
                     GLLRELDTTG"
     gene            complement(1573..3180)
                     /locus_tag="AC16CM_01920"
     CDS             complement(1573..3180)
                     /locus_tag="AC16CM_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3138611.1"
                     /translation="MFRAKYAIAFTLALVMAFGAVGVASAAVPSDSAKLRKAVTVGGI
                     LEHERELQAIADDNDGTRASGTPGYTASADYVAGQLEDAGYVVRRQEFDYELFIENTD
                     PVLDPTDPDLPAYIPGEDFTTMDYSGSGNVEAELQAVGGIVIPSSGGSTSGCSEDDFA
                     DFEAGNIALIQRGTCDFRVKVENAEAAGAVGVVIFNEGNEDPDDDRIGLINGTLDPPQ
                     VDIPVVGTTFAVGEELYNLLQEGPVTLNLAVDAKVETRTTENVIADTPTGRADRTVVV
                     GAHLDSVQEGPGINDNGSGTATDLEVALQMSELGIKPRNKVRFAFWGAEESGLIGSQY
                     YVDSLTKRQLKNIAVNLNFDMVGSPNYVRFVYDGDASDTDSLGSTGSGVVEDVFNDYF
                     ASQGLATEPTAFDGRSDYDAFVSAGIPAGGLFTGAEDIKTAREARIYGGVAGVDYDPC
                     YHAACDSLTPVADGADPATYRAIREAYSGKLVGNLNTRALREMSDAVAHGTVTFAQTT
                     SAVNGTDKGSGTASTTAYKYKGSLLQK"
BASE COUNT          524 a         1042 c         1063 g          605 t
ORIGIN      
        1 gatgttggtg gagcccccag tggccgcacc ggcggcgatg ccgttgtcga acgcctcgcg
       61 ggtcaggata cggtccggcg tgaggccgcg ctcgagcatc tccatcacga gttgcccggc
      121 ctgggtgcag accacgtcct tgtggccgtc gagggccggc gggccagcgg agcccatcgg
      181 cgagaggccg aggaactcaa gtaccatggc catcgtgttg gccgtgtact ggccgccgca
      241 ggcgccagcg ccggggcagg cgtgttcctc gaggtcgagg aggtcctcgt ccgacatctt
      301 cccggcggcg ttcgcgccga cgccttcgaa gacgtcctgg atggttacgt cgtggccctt
      361 gaagcggcca ggggctatgg agcccgagta gacgatggtt ccgggtatgc cgagcctcgc
      421 gtgggccatg gcagcggccg ggagggtctt gtcgcagccg acgatggtga ccagcgcgtc
      481 gaacatgtgg ccgcgcccaa cgagctctat ggagtcggcg atcacctccc tgctcacgag
      541 gctggtcttc atcccctgcg ttcccatagt tatgccgtcg gagatggcga tggtgttcag
      601 ctccatcggc gtgccgccgg cctccctgat gccgtccttg accttctcgg ccaggtgacg
      661 gtggttgaag ttgcagggca tggtttcgat ccacgagtgg gccacgccca ctatgggttt
      721 gctcaggtcc tcatcggaga accccatccc cttgagaaag gacctcgccg gcgcccggtc
      781 gcgcccttcg aggatcgtac ggctcctgtg cctgaggtcg gtcgtctcca tgcctgtctc
      841 cctgccggtg gtcatacgtc ggtccccgag tatagagagc ggagccggcc gatgccagct
      901 aacccggacg ggcacccttg cgccaccagt tgctctgcga tgccggctcc gggagtagaa
      961 tcgaacgata agggatccgg acgaggagta gatatgcaga cgagcggaag agccgggcgg
     1021 gtcatcgcac caggaacccg catcggtcac gtccacctca aggtcgcaga tatcgagcgg
     1081 gcgcttggct tctacaggga tgttctcggc ttcgagatta cccagtggta cggcgaggat
     1141 gcggtgttcc tctcggcggg cggataccac caccacatag gcctgaacac ctggatgagc
     1201 cgcaacgccg accccgcccc gacgcgctcc gccgggctct tccatctcgc cattctctac
     1261 acggaacggc gcgacctcgc acaggcgctg aggtggctgg tcgattccgg gtaccctatc
     1321 gagggggcgt ctgaccacgg cgtctccgag gccctgtacc tgagcgaccc cgacggtaac
     1381 ggcgtcgagc tgtactggga ccgtccagag gaagagtggc ctcgtacccc cgaggggggg
     1441 ctcgcgatgt tcacccgtcc ccttgacgta gacgggttgc tccgggagct ggacacaaca
     1501 ggttgagcaa gagaaaaggg agcggcgaaa agccactccc tttcgacccg cgggcccagg
     1561 cccggcgtgg tgttacttct gcaggagcga ccccttgtac ttgtaggccg tggtgctcgc
     1621 ggtccccgag cccttgtccg tgccgttgac cgccgaggtg gtctgggcga aggtcacggt
     1681 gccgtgggcg accgcgtcag acatctcgcg cagggccctg gtgttgaggt tgccgaccag
     1741 cttgccggag taggcttccc tgatggccct gtaggtggcg gggtcagccc cgtcagcgac
     1801 cggagtcagg ctgtcgcagg ccgcgtggta gcagggatcg tagtccacgc cggcaacgcc
     1861 gccgtagatt ctcgcttccc gggccgtctt gatgtcttcg gcgccggtga aaaggccgcc
     1921 cgccgggata ccggcgctga cgaacgcgtc gtagtccgag cgtccgtcga acgcggtggg
     1981 ctccgtggcg aggccctgcg aggcgaagta gtcgttgaac acgtcctcga ccacgccgga
     2041 gcccgtggag ccgagcgagt cggtgtcgga ggcgtctccg tcgtagacga agcggacgta
     2101 gtttggagat ccgaccatgt cgaagttcag gttgacggcg atgttcttga gctgccgctt
     2161 cgttagcgag tccacgtagt actgcgagcc gatcaggccg gactcttcag caccccagaa
     2221 cgcgaagcgg accttgttgc gcggcttgat gccaagctcg gacatctgca gggcgacctc
     2281 gaggtccgtc gccgtgccgg agccgttgtc gttgatgcct ggaccttcct gcaccgagtc
     2341 aaggtgcgcg ccgacgacca ccgtgcggtc cgcacgcccg gtcggggtgt cggcgatcac
     2401 gttctccgtc gtccgtgtct cgaccttggc gtccaccgcg aggttcaggg taaccggccc
     2461 ttcctgcagg aggttgtaaa gctcctcgcc aacggcgaac gtggttccga ccacgggtat
     2521 gtccacctgc ggcggatcga gcgttccgtt gatgaggccg atccggtcgt cgtcgggatc
     2581 ctcgttaccc tcgttgaaga taacgacacc cacggcgccg gctgcctcgg cgttttcgac
     2641 cttcaccctg aagtcgcagg ttccgcgctg gatcagggca atgttaccgg cctcgaagtc
     2701 ggcaaaatcg tcttccgagc acccgctcgt ggatccgccg gaagacggta taacgatacc
     2761 gcccacggcc tgaagctccg cctcgacgtt accgcttccc gagtaatcca tcgtggtgaa
     2821 gtcctcgccg ggtatgtagg cgggaagatc agggtccgtc ggatcgagca cgggatcggt
     2881 gttctcgatg aacaactcgt agtcgaattc ctggcgcctg acgacgtagc cggcgtcctc
     2941 gagctggccg gcgacgtaat ctgccgacgc ggtgtacccc ggggttcccg atgcccgcgt
     3001 accgtcgttg tcgtcggcaa tagcctggag ctccctctca tgctccagaa tgcctcccac
     3061 cgtgaccgcc ttgcgtagtt ttgccgaatc ggatggcacc gcagccgagg cgaccccgac
     3121 tgccccgaag gccatcacca gcgccagggt gaaggcgata gcatactttg ctctaaacat
     3181 acccctcctc gtgtattcct cgtttgccct gcttcccaca gggaccccga aaac
//
LOCUS       DBNGVD010000067         2527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_193661, whole genome shotgun sequence.
ACCESSION   DBNGVD010000067 DBNGVD010000000
VERSION     DBNGVD010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_193661"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="AC16CM_01925"
     CDS             complement(<1..573)
                     /locus_tag="AC16CM_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3138612.1"
                     /translation="MDPGLLLRGVVLGFAVAAPVGPIGVLCIRRSLAEGRTAGFVVGL
                     GAATADAVYGAVAGFGLTIVSTFLVDQRVWLRLVGGLFLCYLGLRTLVARPSEEAAGG
                     GSGGGLIGTYASTFFLTLTNPTTILSFAAIFAAVSVRTGALTVVPLIVGVFLGSASWW
                     LLLSTGASLIRDRVGPRGMRAVNIVSGAAIL"
     gene            complement(580..1044)
                     /locus_tag="AC16CM_01930"
     CDS             complement(580..1044)
                     /locus_tag="AC16CM_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3138613.1"
                     /translation="MDIQDIQALRLLMREGRSTWSELARLLGLSAPAAADRVRRLEER
                     GVIRGYSAVVDPEAVGLGVTAFVAVTLERPEHRAGFLGWVDGAPEVQECHHVAGEGDF
                     LLKVRCAAVRDLEAIVSEEIKGLHGVTSTRTTVVLSTRKETPVLPLPPPPGG"
     gene            complement(1201..2364)
                     /gene="larC"
                     /locus_tag="AC16CM_01935"
     CDS             complement(1201..2364)
                     /gene="larC"
                     /locus_tag="AC16CM_01935"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR3138614.1"
                     /translation="MTHVHFQPVGGAAGDMTLAALVDAGAPLPEVTRSLTGLDVAFDL
                     STERTLVNGVAALRARVAYPQEHAHRTFADIRGMIEAADLPERASSRALDAFSRLAVA
                     EGTVHDKDPQEVTFHEVGAVDSVVDVVGSCVALELLDAASVSCAPLPMGTGVVDSAHG
                     PLPVPGPATLEILKGSRLRWTEESRETTTPTGAALMTSFTGGEFTDAAPPMTLTGTGY
                     GTGTARLRGAPNLLRAAVGEIEGPAGGVELLEANVDDATGELLGAAMDRVLAAGALDA
                     WLEPIFMKRGRGAYKACALVLSGDIERMARLLMRETGALGLRHHPVGRTTAGRRLVQV
                     ELPYGRCRVKVGSLDGEDFIVSPEYADAARLASETGLPLPVVYEDARIAFGRA"
BASE COUNT          462 a          890 c          809 g          366 t
ORIGIN      
        1 aaggatcgcg gcccccgaga cgatgtttac cgccctcatg ccgcgcggtc caaccctgtc
       61 cctgatgagg ctcgcccccg tgctcaagag caaccaccac gaggccgaac cgagaaacac
      121 gccgacgatc aacgggacca cggtcagggc ccccgtcctg acgctcacgg cggcgaagat
      181 cgcggcgaag gacaggatgg tcgtggggtt ggttagcgtc aggaagaacg tggaggcgta
      241 ggtgccgatc aatcctcccc cgctcccccc tcccgcggct tcctctgagg gccgcgccac
      301 gagcgtcctg agacccaggt agcagaggaa caagccacct accagccgca gccacacccg
      361 ctggtccacc aggaacgtcg agacgatcgt gagcccgaag cctgccacgg caccgtaaac
      421 ggcgtcggcg gtcgccgcgc ccaggccaac cacgaagcca gccgtgcgcc cctcggcgag
      481 cgagcggcgg atgcacagga ccccgatggg accgaccggc gccgcgaccg cgaacccgag
      541 gaccactccc cgcaaaagga gaccaggatc catcgcgtgt tatccccccg ggggaggggg
      601 aagaggcagc acgggtgtct ccttccgggt ggaaaggacc accgtcgtgc gcgtcgaggt
      661 caccccgtgc agacccttga tctcctcgct cactatcgcc tcgagatccc gcaccgcggc
      721 gcaacgcacc ttcaagagga agtcgccctc gccggcgacg tggtggcact cctgtacctc
      781 gggtgcacca tccacccacc ccagaaaccc ggccctgtgc tccggcctct caagcgtgac
      841 ggccacgaaa gccgtcacac ccagcccgac ggcctcgggg tcgaccaccg ccgaatagcc
      901 acgtatcacc ccgcgctcct cgagcctcct cacccggtca gccgcagccg gggcagagag
      961 accaaggagc cgcgccagct ccgaccacgt gctcctgccc tcacgcataa gaagccgtaa
     1021 agcttgtata tcctgtatat ccatctcata atcctagttt tagtaagcaa gaaagtcaaa
     1081 atactttcga aaataaaaaa tcaaccaggc aacctacgaa aagctcgatc ccaaggaggc
     1141 agagtggtca gctatcagct tgagaagaga agctgacagc ggaaagctga tagctgaagg
     1201 ctaggcccta ccaaacgcga tcctggcatc ttcgtatacc acgggcagtg gcagccccgt
     1261 ctccgaggcg agcctggcgg cgtcggcgta ttcgggagag acgatgaagt cctcgccgtc
     1321 gaggcttcca accttgaccc ggcatctccc gtagggaagc tccacctgca ccaggcgtcg
     1381 cccggcggtg gtgcgcccga ccgggtgatg acggaggcca agggcacccg tctcccgcat
     1441 caggagcctg gccatacgct cgatgtcccc cgagagtacg agggcacagg ccttgtaggc
     1501 gccacggccg cgcttcatga atatcggctc tagccaggcg tcgagggccc cggcggcgag
     1561 gactcggtcc atggcggccc cgaggagttc tcccgtggcg tcgtcgacgt ttgcctcgag
     1621 tagctcgacg ccgcccgctg ggccctctat ctccccgaca gcggcccgca gcaggttagg
     1681 cgctccgcgc aaccgcgcgg ttcccgtccc gtagcccgtg ccggtcaggg tcatgggcgg
     1741 agcggcgtcc gtgaactcgc cgccggtaaa ggacgtcatc agcgccgccc ctgtcggcgt
     1801 ggtcgtctct cgggactctt cggtccaacg gaggcgcgag cccttgagga tctccagcgt
     1861 ggccggtcca ggcacgggga gtggcccgtg ggcggagtcg accacgcccg tgcccatggg
     1921 aaggggggcg cagctcacgg aggccgcgtc gagcagctcc agcgccacgc agcttccgac
     1981 aacgtcgacg accgaatcca cggcaccgac ctcgtgaaag gtgacctcct gggggtcctt
     2041 gtcgtggacg gtgccctccg ccacggccag gcggctgaaa gcgtcgaggg cgcgggatga
     2101 ggcccgctcc gggaggtccg cggcctcgat catgccgcgg atgtccgcga aggtcctgtg
     2161 ggcgtgttcc tgcgggtagg ctaccctggc ccggagcgcg gccacgccgt tcaccagggt
     2221 gcgctcggtg gagaggtcga aagccacgtc gaggccggtc agggacctcg ttacctcggg
     2281 taaaggtgcc cccgcgtcga cgagggcggc cagtgtcatg tcgcccgccg caccgccgac
     2341 gggctggaaa tggacgtggg tcagggccgg cctcctggct ccgggatccc cggaccctgc
     2401 cggtggtgtc cctctattcc ccttcgcccc ggccgttgga gatcctacgc accgccatca
     2461 gggtaacggc caggctcacg gccagcagga tcaggggcct cggatcgctc ttcacggcgt
     2521 ccgtgag
//
LOCUS       DBNGVD010000068         4331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2015829, whole genome shotgun sequence.
ACCESSION   DBNGVD010000068 DBNGVD010000000
VERSION     DBNGVD010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2015829"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="AC16CM_01940"
     CDS             <1..1645
                     /locus_tag="AC16CM_01940"
                     /inference="COORDINATES: protein motif:HMM:NF023193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138615.1"
                     /translation="NDGTRFVGEGRFRITDLAEVTTLGLFNVSKETKFELASSSLEGG
                     DGVLRGTGTMDGLGHFLWTGGDLAGNLELRSGIQTYMNGPSTKDLSGTVTNQGRFVLQ
                     AGDSTEPPTGQLIFGPQSKLNNNGLFIADERTGMVGTVCCSLPASFNNTGQFNVRPSG
                     TTTPGTVTVSNLLFKAGGSIHVERGTLEFRKGPGVLGPIDITGKGTVRVTDLASMALT
                     GAFNLDPGATLELGSCSGGCGYGQLTGEGTLGGGGRFKWTGGVIGESGNLTIADDSSI
                     VISGTANKELKGTITNRGKATLISSAASPGGPLAFIGAARFFNEGTFYPGDGSTFMSP
                     VCCVDPAKFVNRGTVIMDRAHTPATGTASVENMVFENRGTVDLASGTLRFDTVGYTQL
                     SGSTRLTGGGLSSDGPIDLKGGMLAGTGKVIGNLHNYDGTVSPGNPASAGSTGTLNVA
                     GSYSQEAQGILKVNLKGSTPGSGFDQLRVSRQAVLDGTMDLDRATAYTPELTTKLAVM
                     TMSSRLGRFDRLQDTTLPNGREWYAAYSPRAVSLGVVRG"
     gene            1812..2228
                     /locus_tag="AC16CM_01945"
     CDS             1812..2228
                     /locus_tag="AC16CM_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138616.1"
                     /translation="MRHRVTLLFLPVCAVFLVLILTACSFSASSASISNAEMARDQDG
                     KQPTETFSPDEPFYCIVELSNAPEDTSVRAVWTAVEVEGADANTRIDEARTTSGSGQL
                     QFNLSNESPWPTGDYKVDLYLNDAEDPTETLEFQVE"
     gene            complement(2304..2660)
                     /locus_tag="AC16CM_01950"
     CDS             complement(2304..2660)
                     /locus_tag="AC16CM_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138617.1"
                     /translation="MAIRLIRLFTGEDGQSHFQVGEIEWSEETALGAISRGEPAHAIS
                     FEETAAGASLDWHNAPHRQYVITLSGCLEFETRKGDKQTIEPGDILLAEDTKGGGHRW
                     RLVDDQPWRRAYITVS"
     gene            complement(2662..3384)
                     /locus_tag="AC16CM_01955"
     CDS             complement(2662..3384)
                     /locus_tag="AC16CM_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3138618.1"
                     /translation="MTKLTLESGVLRVASAFPDPPFEVENDGDQTGFDRELMRLICDH
                     LGLALRPVPYGGDDFEGIFEGLGDGSYDAVISGTTITPERERKALFSAPYLQFDQGLA
                     VNVRRNPGVRSSDDLRGMVVGIQVGNTSDLVARELLARGAIGDIKYYPYHGILDALDD
                     LSAGRVGALIKLFPVLSWLVKDREGLAVIDQFPTHEKIGIAFARTNPGLCRAVNESLE
                     SLRKNGKFESLRSKWFDEPQGD"
     gene            complement(3395..3565)
                     /locus_tag="AC16CM_01960"
     CDS             complement(3395..3565)
                     /locus_tag="AC16CM_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138619.1"
                     /translation="MSAAILFALAISVCAAVVATLLITTWRHEDLVLTTVVAFVLLPV
                     LILAIMAWQEAI"
     gene            complement(3562..3732)
                     /locus_tag="AC16CM_01965"
     CDS             complement(3562..3732)
                     /locus_tag="AC16CM_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138620.1"
                     /translation="MRDLLLLLVAIVAAVGCAYYYVAAVLDGHRALSRSAVLAFFIFS
                     LIVAIYIRRVLW"
     gene            complement(3986..4327)
                     /locus_tag="AC16CM_01970"
     CDS             complement(3986..4327)
                     /locus_tag="AC16CM_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138621.1"
                     /translation="MATPLHRHANEDEYSYVLEGRMGAQLGDDVVIAEEGTLVFKPRN
                     QWHAFWNAGDEPCRILEIISPGGFERLFEETAEPRNTADPPLDVRYGVEFDFESVVRL
                     CREHGLVFPDE"
BASE COUNT          845 a         1386 c         1355 g          745 t
ORIGIN      
        1 caacgacggc accaggtttg tcggcgaggg aaggttcaga atcaccgacc tcgcagaggt
       61 aaccacactc ggcctgttca acgtgtcgaa ggagacgaag ttcgagctgg cctcctcctc
      121 gctcgaaggc ggggacggcg tgctccgcgg cacggggacg atggacggcc tcggtcactt
      181 cctgtggacg ggaggcgacc tcgccggcaa cctcgagctc agaagcggca tccagaccta
      241 catgaacggc ccctccacca aggacctgag cggcaccgtg accaaccagg gcaggttcgt
      301 gctccaggca ggcgattcga ccgagccccc gacgggtcag ctcatcttcg gcccgcagtc
      361 caaactcaac aacaacggcc tgttcatcgc ggacgagcgc accgggatgg tcggtaccgt
      421 ctgctgctcg cttcccgcga gcttcaacaa caccggccag ttcaacgtga gaccctcggg
      481 cacaacgacg cctggcaccg tcacggtgag caacctcctc ttcaaagcgg gcggctccat
      541 ccacgtggag aggggaaccc tggagttcag gaaaggaccg ggtgtgctcg gtcccataga
      601 catcacgggc aagggcaccg tgcgggtaac cgatctggcg agcatggcgc tcaccggcgc
      661 gttcaacctc gatccgggag ccaccctcga gctcggttct tgctccggcg gatgcggtta
      721 cggacaactc acgggtgagg gcacgctcgg cgggggcgga cggttcaagt ggacgggcgg
      781 tgtcatcggc gaaagcggca acctcaccat cgccgacgac agtagcatcg tcatatctgg
      841 caccgccaac aaggaactca agggcaccat caccaacagg ggcaaggcga ccctgatctc
      901 cagcgccgcc tccccgggtg gtccgctcgc gttcatcggg gccgccaggt tcttcaacga
      961 gggtaccttc tatcccggcg acgggtccac gttcatgtcc cccgtatgct gtgtcgaccc
     1021 cgcgaagttc gtgaacaggg gaaccgtcat aatggaccgc gcccacacgc ccgccacggg
     1081 caccgcatcg gtagagaaca tggtcttcga gaaccgcggt acggtggatc tagccagcgg
     1141 cacgctccgc ttcgataccg tcgggtacac gcagctctcc ggctccacgc gccttacggg
     1201 cggcggcctg tcctccgatg ggcccataga cctcaagggc gggatgctag ccggcacagg
     1261 caaggtgatt gggaacctgc acaattacga cggtacggtc tcccccggta acccggccag
     1321 cgccggatct accggcaccc tcaacgtcgc aggcagctac agtcaggagg cgcaggggat
     1381 cctgaaggtg aacctgaagg gctcgacgcc tggcagcggg ttcgaccagt tgcgggtaag
     1441 tcggcaggcc gtactcgatg ggacgatgga cctggacagg gccaccgctt acaccccgga
     1501 gctcaccacg aagctcgcgg tgatgacgat gtcgagcagg ctgggccggt tcgacaggct
     1561 ccaggacacc acgctcccca acggccgcga gtggtacgcc gcctacagcc cccgcgccgt
     1621 atcgctgggc gtagtcaggg gctaaccccc tcgggagggg agggcagcac cgtaccctcc
     1681 cctcccgaga cgtcgcaggc aaaagtccgt agaatgcgga ccgttccggt atggaccagc
     1741 gcgacgctag tttggcgcca ccttcacgcg ctgtcggagg gcgtgtgaac cgaggagacg
     1801 aggagggcaa cgtgaggcac agggtgacgc ttctattcct gccggtttgc gccgtctttc
     1861 tggttctcat cctcaccgct tgtagcttca gcgccagctc ggcaagcatc agcaatgcgg
     1921 agatggcccg ggatcaggac ggcaaacagc cgaccgagac gttctccccc gacgagccgt
     1981 tctactgcat cgtcgagctc tccaacgccc cggaagacac gtcggtaagg gcggtgtgga
     2041 ccgccgtcga agtcgagggc gccgacgcca acaccaggat agacgaggca agaaccacaa
     2101 gcggtagcgg ccagctccag ttcaacctga gcaacgagag cccctggcct accggtgact
     2161 acaaggtgga cctctacctg aacgacgccg aagacccaac ggaaacgctc gaattccagg
     2221 ttgagtaggg cctaaagcta ctagctgtca gccgtcagcc ttcagctcct tggctgatag
     2281 ctagtagcta atggctgaca gctctaggat actgtgatgt aggctcgtcg ccatggttgg
     2341 tcgtcgacca gacgccagcg gtgtccgcct cctttggtgt cttccgcaag gaggatgtcg
     2401 ccaggttcga tggtttgttt gtcgcctttg cgggtctcga actcgagaca tccgctcagg
     2461 gtgatcacgt actggcggtg cggggcgttg tgccagtcca ggctggctcc ggcggcggtc
     2521 tcttcgaagc ttatggcgtg ggccggttcg ccccgggaga tggcgccgag ggccgtctcc
     2581 tcgctccatt cgatctcacc cacttggaag tgggactggc cgtcctcgcc ggtgaagagg
     2641 cggatgagcc tgatcgccac cctaatcccc ctgtggttcg tcgaaccact tgctgcgcag
     2701 agattcgaac tttccgttct tcctgaggct ctccaggctc tcgttcaccg cccggcataa
     2761 cccggggttc gtgcgggcaa acgcgatacc gatcttctcg tgggtgggga actgatctat
     2821 cacggcgagc ccctcgcggt cctttaccag ccacgacaag accgggaaca gcttgataag
     2881 cgccccgacg cgccccgcgg acagatcatc gagggcatcg aggatgccgt ggtagggata
     2941 atatttgata tcccctatgg cgcccctggc cagtagctcg cgggccacga ggtccgaagt
     3001 attgccgacc tggatgccaa cgaccatgcc gcgcaggtcg tccgaggatc tgacccccgg
     3061 gttacgcctc acgtttacgg ccaggccctg gtcgaactgc aagtagggcg cggagaatag
     3121 ggccttccgt tcgcgttcgg gcgtgatggt ggtgcccgaa ataacggcat cgtagcttcc
     3181 gtccccaagg ccctcgaaga taccttcgaa gtcgtctccg ccgtacggca cagggcgcaa
     3241 cgccagcccg agatggtcac agatcagacg catcagctcc ctgtcgaagc cggtctgatc
     3301 gccatcgttc tcaacctcga acggagggtc ggggaaagcc gacgcgaccc ggagcacccc
     3361 agactctagc gttagcttcg tcatggcaag gtccctatat ggcctcttgc caggccataa
     3421 tggcaaggat gagcacgggg aggagaacga aggcgaccac cgtggtcagg accaggtctt
     3481 cgtggcgcca ggtcgttatg aggagggttg cgacgaccgc cgcgcacacg gagatggcaa
     3541 gggcgaacag gatggccgcg ctcaccagag aacgcgcctg atatagatgg ccacgatcag
     3601 gctgaagatg aagaaggcaa ggacagccga cctggacaga gcccgatgac cgtcgagcac
     3661 ggcagccacg tagtagtagg cgcagcccac cgccgcaact atagcgacca gcagaaggag
     3721 gagatcccgc atcctactct cctagcccgg tcccgccccg aaggcgccgt ttccgtactc
     3781 cagatcccta cagatcctgc ttctggatcg gggagtatcc tgctcgccca cgtaaccgtc
     3841 ctcccgagta tccgcgctcc ccacgggcta tagactaata ccaggtcggg atgaacgtca
     3901 acgtgttacc cgcgattttg gcccttcgtg ggttcccgtt tccgcggtaa tgacgggtgc
     3961 gaagaaccgg gcactatcgt ctcttctact catcggggaa gacgaggccg tgttcccggc
     4021 agaggcgtac tacgctctcg aagtcgaact ccacgccgta gcggacgtcg aggggtgggt
     4081 ccgccgtatt gcggggctcc gccgtctctt cgaagagtct ttcaaaaccg ccaggggaga
     4141 tgatctccag tatgcggcac ggctcatcac cggcgttcca gaaggcgtgc cactggttgc
     4201 ggggcttgaa gacgagggtg ccctcttccg cgatgacgac gtcgtcgccg agctgcgcgc
     4261 ccatgcgacc ctccagtacg tagctgtact cgtcctcgtt ggcgtggcga tgaagcgggg
     4321 tagccagcgt c
//
LOCUS       DBNGVD010000069         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2744671, whole genome shotgun sequence.
ACCESSION   DBNGVD010000069 DBNGVD010000000
VERSION     DBNGVD010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2744671"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="AC16CM_01975"
     CDS             complement(<1..585)
                     /locus_tag="AC16CM_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138622.1"
                     /translation="MTDKFTRRRFLTVLGAGSACLAAGGIAGLVLFGHDQEKVSFLQA
                     PSTGPLRAPEVWPLPGASPENPSGPWHFRSRPDLRPPAVEVTVPAHGTAPGHIFVAPQ
                     EGDGGQGGALVVDDGGQVAWFHPMQSPSSRAMNFLAQTYRGEPVLTWGETPGVYVIYD
                     SSYREIARFEAANGFNGDHHEFLISPQDTALITIY"
     gene            complement(638..862)
                     /locus_tag="AC16CM_01980"
     CDS             complement(638..862)
                     /locus_tag="AC16CM_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138623.1"
                     /translation="MIKTIVITGAIGMIVGFGGTLMSLFGGGTLLMEYLEFVVMLVLF
                     LVMLIGAYWFVMVNPSSEEKEPATEKSLGT"
     gene            complement(1023..2693)
                     /locus_tag="AC16CM_01985"
     CDS             complement(1023..2693)
                     /locus_tag="AC16CM_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3138624.1"
                     /translation="MSDRMDEIRSTRAPRLRTDQIKILKRYGHTRTTEVGDVLFRAGD
                     TENDFVVVLEGEVKVGDDFPGEWRTIGTFHEGRFLGELNMLTGQAMYLTGVVSEAGEA
                     LFITRERLKEVVTEEPNISDIILRAFLARRSYMMKEGLGLRIIGSRHSGDAARLREFA
                     ARNRVPHVWMGLEDDPRAGALLESLSAKPSETPVTVWQGEDVLKNPTNLQFARATGLR
                     VDAPLQRTYDLVVIGAGPAGLGASVYGASEGLSTLALESVALGGQAGTSSRIENYPGF
                     PAGLSGFELTSRVLVQADKFGARTAVPQEATGLRREDNRFIIGLSEGGEVVARSIIVA
                     SGARYRRLDVESLGQFEGESIHYAATQAEAQRCEGEEVAVVGGGNSAGQAALFLAAHT
                     RKVHLLIRGDDLGKSMSSYLVDRVMNTPNIELLANTEVRVLMGEDRLAGVVVERGHPG
                     ELQTLGARFLFVFIGADANTDWLEGTVGLDERGFVLTGTELDRSVLDEEDWRQRSREP
                     FLLETSMPGVFAAGDARSGSIKRCASAVGEGAMAVRLVHQYLAAEPTG"
     gene            2855..3601
                     /locus_tag="AC16CM_01990"
     CDS             2855..3601
                     /locus_tag="AC16CM_01990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138625.1"
                     /translation="MHLDENPFAAEAEDYAHLRPTYPDSLFTFLATVAPARGAAWDCA
                     TGNGQAATHLAEHFDRVVATDESGEMIAQAPPDPRITYRVAEAEESGLEDGSVDLVTV
                     ASAIHWFDLERFYAEVNRVVKPGGAVAAWTYYTPVFGNEVDPIIRRLAHDILGPYWDE
                     RVHYVVDEFHDLPFPFEPIEAPSFRTDMTWDMRDLLGYFETWSSSIKYRETNHESPTR
                     LIEDDLARAWGDPQQRQDLHFPLYMRLGKV"
     gene            complement(3649..>3875)
                     /locus_tag="AC16CM_01995"
     CDS             complement(3649..>3875)
                     /locus_tag="AC16CM_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3138626.1"
                     /translation="LIFGGIAAVSFTYLFGLESTWAHRLMVVTLAAVIGLVLFTVGAL
                     GHPFEGGARIGTEAFNLILERFETSKLSDL"
BASE COUNT          678 a         1317 c         1186 g          694 t
ORIGIN      
        1 gtagatcgtg atcagcgccg tatcttgcgg gctgatcaag aactcgtggt ggtccccgtt
       61 gaagccattt gcggcctcga agcgggcgat ctcacggtag gaggaatcgt agatgacgta
      121 cactccgggc gtctcgcccc aggtgagcac gggctcccca cggtaggtct gcgccaggaa
      181 gttcatggcc cggctggacg ggctctgcat gggatggaac caggctacct gccccccgtc
      241 gtcgacgacc agcgcccccc cctgcccgcc gtcgccctct tgcggggcga cgaagatgtg
      301 tcccggggcc gtaccgtgtg ccggcacggt cacctcgacg gcgggagggc gcaggtcggg
      361 gcgggagcga aagtgccacg gtcctgaggg gttctccggc gaagcgcctg gcaacggcca
      421 gacctcagga gcccgcaagg ggccggtact cggggcttgc aggaagctta ccttctcctg
      481 atcgtgtccg aacaggacga ggccggcgat accgcccgcg gccaggcagg ccgatccggc
      541 gcccagcacg gtgaggaagc ggcgtcgcgt gaacttgtct gtcatccctc tatccagttc
      601 gctctgcggg gcgggctcat atctctggga ataggggcta cgttcccagg ctcttctcgg
      661 tcgctggttc cttctcttca ctcgaggggt tgaccattac gaaccagtaa gcaccgatca
      721 gcatgacgag gaacaggacc agcatcacga caaactccag atactccatc aacaaggtac
      781 cgcccccgaa cagggacatg agcgtaccgc cgaaaccgac gatcatccct atggcaccgg
      841 tgatgacgat tgtctttatc atgtgctcct ttccgaggcg ctcgtaaggc cacaaaaacc
      901 tccccgtggc gccctgtcag gggggcctcg acgatctcca acccgggtaa ttatgcatgg
      961 gttcgtgacc tcttgcatct gccaggtggc catagttggc gacctgcggg ccatgagggt
     1021 ctttaccccg tcggctctgc tgccaggtac tggtgaacca accttacggc catggcgccc
     1081 tcgcccaccg cggaggcaca acgcttgatc gagcccgagc gcgcgtcgcc cgccgcgaac
     1141 acccccggca tgctcgtctc cagcaagaaa ggctccctcg aacgttgccg ccagtcttcc
     1201 tcgtcgagca cggagcggtc cagctccgtc cccgtcagga cgaaaccgcg ctcgtcgagc
     1261 ccgacggttc cctcgagcca gtcggtgttg gcatcggcgc cgataaagac gaagaggaaa
     1321 cgcgccccca gggtctggag ctcacccggg tgtcccctct cgaccactac accggccagg
     1381 cggtcctccc ccatcaatac cctgacctcc gtgttggcga gcagctcgat gttgggagtg
     1441 ttcattaccc tgtcgaccag gtagctcgac atgctcttgc ccaggtcatc gccccttatg
     1501 agaaggtgga cctttctcgt gtgtgcggcc aggaacagcg cggcctgacc tgccgagttg
     1561 ccccctccga cgaccgcgac ctcctcaccc tcgcagcgct gcgcctcggc ctgcgtcgcg
     1621 gcgtagtgga tgctttcgcc ctcgaactgc ccgaggcttt ccacgtcgag cctgcggtag
     1681 cgggccccac tcgccacgat tatgctgcgc gccacgacct caccaccctc ggagagcccg
     1741 ataatgaagc gattatcctc cctgcgcagg ccggtcgcct cctggggaac ggccgtgcgg
     1801 gcgccgaact tgtcggcctg cacgaggacg cggctggtca gctcgaagcc ggagagcccg
     1861 gcgggaaacc cgggatagtt ctctatgcgg gagctggtgc cggcctgtcc tccgagggcg
     1921 accgactcga gcgccagggt tgagaggccc tccgaagccc cgtagaccga cgcgccgagc
     1981 ccggccgggc cggccccgat gacgacgaga tcgtaggttc tctgcagggg ggcatcgacc
     2041 ctgaggcccg tggcgcgggc gaactgcagg ttggtcgggt tcttcaaaac gtcctcgccc
     2101 tgccagacgg tcacgggcgt ctccgacggt ttcgcgctga ggctttcgag cagagcgccg
     2161 gctctcgggt cgtcctccag tcccatccag acgtggggaa cgcggttgcg ggccgcgaac
     2221 tccctgaggc gcgccgcgtc accagagtgg cgcgagccta taatcctcag gccaaggccc
     2281 tccttcatca tgtacgagcg tctggccaga aacgccctga ggatgatgtc cgagatgttt
     2341 ggctcctcgg tgacgacctc cttcaggcgc tcgcgggtta tgaacagcgc ctccccggcc
     2401 tcgctcacca cgcccgtcag gtacatggcc tgccccgtga gcatgttcag ctcgccgagg
     2461 aagcgtccct catggaaagt ccctatggtt cgccactcgc cggggaagtc gtcgcccacc
     2521 ttcacttcgc cttccaatac gaccacgaag tcgttctcgg tatcgcccgc cctgaacagg
     2581 acgtcgccga cctcagtggt gcgcgtgtgc ccgtagcgct tgaggatctt gatctggtcc
     2641 gtcctcagcc tcggggccct ggtgctgcgt atctcgtcca tacgatcgct catgccacgc
     2701 cctcaccgat agcttttccc catgcaacca tgatgcctgc tgcgccagcc cggcgcatct
     2761 tcttgctgga ccatgcctgg gtacgccctc gtccgtgacg gatctggcgc tctgatatag
     2821 tggctttccc gctatgaatc aggaggaggg ccccatgcat ttagacgaga accctttcgc
     2881 cgccgaggcc gaagattatg ctcacctccg ccccacctat ccggatagcc tcttcacgtt
     2941 cctggcgacc gttgcgccag cacgcggggc cgcctgggac tgcgccacgg gcaacggaca
     3001 ggcagccacg cacctggcgg agcacttcga ccgggtcgtg gcaaccgacg agagtgggga
     3061 gatgatcgcc caggccccgc ctgacccccg aatcacctac cgggttgccg aagcggagga
     3121 gtcgggcctg gaagatggtt ccgtggacct cgtgacggtg gcttccgcta tccactggtt
     3181 cgacctcgaa aggttctacg ctgaggtaaa ccgcgtggtg aaacctggcg gcgccgtggc
     3241 ggcctggacc tactacaccc ccgtattcgg aaacgaggtc gatcccatca tccggcgcct
     3301 ggcacacgac atcctgggtc cttactggga cgagcgcgtg cactacgtgg ttgacgagtt
     3361 ccacgacctg ccctttccat tcgagccgat agaggcaccc tccttccgga cggacatgac
     3421 gtgggacatg cgggaccttc taggctactt cgagacgtgg tcatcctcga tcaagtaccg
     3481 cgagaccaac cacgagagcc ccacgcgcct gatcgaggac gacctggcga gggcgtgggg
     3541 ggacccgcag cagagacagg acctccattt cccgctctac atgcgcctgg gaaaggtgta
     3601 acgggcggtc ctgaataggg tgagttcgaa acccgcccta cgggatcgtc agaggtcgct
     3661 tagcttgctg gtctcgaacc tctccaggat caggttgaac gcctccgtgc cgatgcgggc
     3721 tcccccctcg aaagggtgcc ccagggcacc tacggtaaag agcacgagcc ctatcacagc
     3781 cgccagggtc accaccatga gcctgtgcgc ccacgtgctc tccaggccga agaggtaggt
     3841 aaagctaacc gcggcaatgc ccccgaagat cagga
//
LOCUS       DBNGVD010000070         5453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4567260, whole genome shotgun sequence.
ACCESSION   DBNGVD010000070 DBNGVD010000000
VERSION     DBNGVD010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4567260"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1329
                     /gene="clpX"
                     /locus_tag="AC16CM_02000"
     CDS             70..1329
                     /gene="clpX"
                     /locus_tag="AC16CM_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3138627.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQEDAKKVLAVAVYNHYKRIQMGAEATDG
                     TELQKSNILLVGPTGSGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNVLFICGGAFGGLEEIIRQRIGKKSIGFGSDIDSRLRQED
                     DSILQFALPEDLLKYGLIPEFVGRLPVISTLRSLEEADLVRILTEPKNALVRQYRQFF
                     RYDKVDLSFTDEALAGIAELALERGTGARGLRSILETALLPTMYELPSRSDVNKCVVD
                     VDTVKEGVQPSLVTGSQKYSYDEEETA"
     gene            1452..3998
                     /locus_tag="AC16CM_02005"
     CDS             1452..3998
                     /locus_tag="AC16CM_02005"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3138628.1"
                     /translation="MSQQIPKNYDHAAVEDRWYAEWERAGVFEADPDPDKEPYCIVMP
                     PPNVTGALHMGHALNGSIQDALTRRARMKGYEALWLPGLDHASIALQNVVERNLMRDE
                     GKSRFDVGREDFVERCRDFADESRSQILGQLKRLGASVDWRRLRYTMDERYVDAVLTA
                     FVDLFNEGLIYRENRITNWCPHDRSAISDLEVNYEDVDGKLYALRYPLVDGLGPGPDG
                     KDYAQVFSTRPETMLGDTALVVNPHDGRYTDLVGRRVLVPFVDREIEVLADDYVDPSF
                     GTGVLKVTPAHDPNDFEIGQRLGLASVNVLNPDGTVSENGGPFAGLDRVEARSAVAAR
                     LDDDGLLGEVRDYRHRVGHCDRCGAVIEPWLSEQWWCSMRELAEPAIKALENGEITVY
                     PDSWRRETIRWLENIHDWNVSRQLWWGHRIPVWYGPAGEVIASKESPGEGWEQDADIL
                     DTWFSSGLWPFATLGWPEEGEDLVYFYPTTLLSTAREIMYLWVARMIMMGLRFREGIP
                     FGKVNVHSIVLAEDGTKMSKSKGNTINPLDLFDQYGTDAVRFGLLYQSSTQDFAYSYE
                     RAEMGRAFVTKLWNATRFVLSYPEAEEPGDPSPADRWILSEFNRTVRDYDAFLEECEF
                     SEAMRRIYSFAWNQFADWYIEIAKATPSTATPRVLREVFSGILRLLHPVMPFATEEMA
                     SIMGEEGLLATQRFPEYDPALEDEDADGLLDRTKRAVSAVRSFRAESKVEGGLEGVLP
                     DGIDEGVFAALAGVKPVETPDGASRASLPAGDLVVEILLSEEMRRGEIERLRREISHA
                     EKEVKRSEGKLANEKFVQNAPEKVVAGEREKLDVNAGMLETLRRRLDEYL"
     gene            4046..5242
                     /locus_tag="AC16CM_02010"
     CDS             4046..5242
                     /locus_tag="AC16CM_02010"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3138629.1"
                     /translation="MDRRRHITLGFERIEALLEAMGHPEREIPHIVQVVGTNGKGTTA
                     VALAGALEASGRPSGAYLSPHVLSYTERVVLWGEYSSEDRFAQAMGETIELADRHGIG
                     VSQFEILTAGALRMFADADVSWAVLEAGLGARHDATSVAGPEAVVLTNVALDHTEYLG
                     DTIEEIAAEKLASLRPGATLYLGSPDERVVGLARDTCRRVGANLVEVADPPHITAPAG
                     LAPYAVSNVELGVSVAESLMGESLSGETRESVASDVVGTLPGRFEVREMGGVPVILDG
                     GHNVAGVAAALASVRSAFPDRPFGVVFGVLRDKDAAGMLSGFVRGAQMIVLTRPEGER
                     ASDPGRVGSEHDPLDREGRRALVVPDPVEALGAAVKAMRETGGVVLVTGSLSTIAPVS
                     RWLREE"
BASE COUNT         1001 a         1719 c         1798 g          935 t
ORIGIN      
        1 atcgacaaca tcgtcaggaa ccactagagg tctcgggagg cgatacggat gtcggggagg
       61 atgtaacgga tgagggatcc ttcggatcag ctgcagtgct cgttttgcgg taagagccag
      121 cgccaggtca ggaagctcat agccggcccc ggcgtctata tctgcgacga gtgcatcgag
      181 ctgtgcaacg agatcatcga cgaggagttc tccggccccg aggtcttgaa ggacgatgac
      241 ctccccaagc cgcgggagat caaccgcatc ctcaacgagt acgtcatagg ccaggaggac
      301 gcgaagaagg tactggccgt cgccgtctac aaccactaca agcgcatcca gatgggtgcg
      361 gaggccacgg acggcaccga gcttcagaag tcgaacattc tgctggtcgg tcccaccggc
      421 tcgggcaaga cgctcctggc ccagacgctg gccaggatcc tgaacgtccc gtttgccata
      481 gcggacgcca cggcccttac agaggccggc tacgtgggcg aggacgtgga gaacatcctt
      541 ctcaagctca ttcaggccgc cgacttcgac gtgaagaagg cggagaccgg gatcatctac
      601 atcgacgaga tcgacaaggt agcccgcaag gcggataacc cttcgatcac ccgcgacgtc
      661 tcgggcgagg gcgtgcagca ggctctgctg aagatcctgg agggcaccgt ggcctccgtg
      721 ccgccgcagg gcgggcgcaa gcacccgcac caggacttcc tgcagataga caccaagaac
      781 gtactgttta tctgcggcgg tgcgttcggc ggcctggagg agatcatccg ccagcgcatc
      841 ggcaagaaga gcattggctt cggctccgac atcgactcgc gcctgaggca ggaagacgac
      901 tccatccttc agttcgccct tcctgaggac ctgctcaagt acggcctcat ccccgagttc
      961 gtgggtcgtc tgccggtcat ctccaccctg cgcagcctgg aggaggccga cttggtgcga
     1021 atcctcaccg agcccaagaa cgccctcgta cgccagtaca ggcagttctt ccgctacgac
     1081 aaggtggacc tctccttcac ggacgaggca cttgccggta tagcggagct cgcgctggag
     1141 cgcggcaccg gagcccgtgg cctgaggagc atcctggaga cggcgcttct gccgaccatg
     1201 tacgagctcc cgagccgctc cgacgtgaac aagtgcgtgg tagatgtgga cacggtcaag
     1261 gaaggtgtcc agccgtctct tgtaacgggc tcccagaagt actcctacga cgaagaagag
     1321 accgcctagg ggtgtctttc gccccagaac gtaggggcgg gtttcgaacc cgccctctac
     1381 gggcgccgta agcagagaag cggcgcccac acgggcggcc cctgtcaaac gattcgaagg
     1441 tacctctttc cttgagccaa cagattccga aaaactacga ccacgcggcg gtcgaggacc
     1501 gctggtacgc cgaatgggag cgggccgggg tgttcgaggc cgaccccgac ccggataaag
     1561 agccctactg catcgtgatg ccgccgccca acgtgacggg cgcgcttcac atggggcatg
     1621 cgctcaacgg ctccatccag gacgccctca ccaggcgcgc caggatgaag ggctacgagg
     1681 ccctgtggct accaggactc gaccatgcct ccatcgcgct tcagaacgtg gtggagcgta
     1741 acctcatgcg tgatgagggc aagagccgct tcgacgtcgg ccgcgaggac tttgtggagc
     1801 gttgccgcga cttcgccgat gagtcgcggt cccagatcct gggccagctc aaacgcctcg
     1861 gggcctccgt ggactggcgc aggctccgct acaccatgga cgagcggtac gtcgacgccg
     1921 tgctcacggc cttcgtggac ctcttcaacg agggcctgat ctaccgcgag aaccgcataa
     1981 ccaactggtg cccgcacgac cgatccgcga tcagcgacct cgaggtcaac tatgaggatg
     2041 tagacggcaa gctctacgcc ctgcgttacc ccttggtcga cggcctgggg ccaggtccag
     2101 acgggaaaga ctacgcccag gtattctcca cgcggcctga gaccatgctc ggcgacacag
     2161 ccctcgtcgt caacccacac gacggacgtt acaccgacct ggtgggacgc agggtgctgg
     2221 tcccgttcgt ggaccgcgag atcgaggttc tggccgacga ctacgtcgac ccgagcttcg
     2281 gcaccggtgt gttgaaggtt acgccagccc acgacccgaa cgatttcgag atcggtcagc
     2341 gtctcggcct agcttctgtc aacgttttga acccagacgg cacggtcagc gagaacgggg
     2401 ggccattcgc cggcctcgac cgcgtggagg cgaggagcgc cgtcgccgcg cgcctggacg
     2461 atgatggcct gcttggcgag gtgcgcgact acaggcacag ggtcgggcac tgcgaccggt
     2521 gcggggccgt catagagcct tggctctccg agcagtggtg gtgctccatg cgggagctgg
     2581 ccgagcccgc cataaaggct ctcgagaatg gagagattac cgtctacccg gattcctggc
     2641 gcagggagac catacgctgg ctcgagaaca tccacgactg gaacgtgtcg cgccagctgt
     2701 ggtggggcca ccgcatccca gtgtggtacg ggcccgccgg ggaggtcatc gccagcaagg
     2761 agtcgcccgg cgagggctgg gagcaggatg cggacatcct cgacacgtgg ttctcgagtg
     2821 gtctgtggcc cttcgccacg ctcggttggc cggaggaggg cgaggacctc gtgtacttct
     2881 acccgaccac gttgctctct acggcgcgcg agatcatgta cctctgggtg gcgcggatga
     2941 tcatgatggg attgcgcttc cgggagggga ttcccttcgg gaaggtgaac gtccactcca
     3001 tcgtgctcgc cgaagacggc accaagatga gcaagtcgaa aggcaacacc ataaacccgc
     3061 tcgacctctt cgaccagtac ggcacggacg cggtccgctt cggactcctc taccagtcct
     3121 ccacccagga cttcgcctac tcctacgagc gggccgagat gggccgggcc ttcgtcacca
     3181 agctctggaa cgcgacccgt ttcgtgctct cctatcccga agccgaggag cccggcgacc
     3241 cttctcctgc tgaccgttgg atcctctcag agttcaaccg caccgtgagg gactacgacg
     3301 cattcctcga agagtgcgag ttctcggagg cgatgcgacg catctactct ttcgcctgga
     3361 accagttcgc cgactggtac atcgagatcg cgaaagcgac accgtccacg gccacccccc
     3421 gcgttctgcg cgaggtgttc agcggcatcc tcaggcttct gcacccggtc atgccgttcg
     3481 ccacggagga gatggcaagc atcatggggg aggaagggct gctcgcgacg caacgcttcc
     3541 ccgagtacga ccccgccctc gaagacgagg acgccgacgg gctgctcgat cgtacgaaac
     3601 gtgccgtctc cgcggtccgc tctttccgtg ccgagtcgaa ggtcgaaggc gggctcgagg
     3661 gggtcctgcc ggatggcatc gacgagggcg tcttcgccgc gctcgccggg gtaaagcccg
     3721 tggagacacc cgacggcgcc agccgcgcct cgttgccagc cggggacctg gtcgtcgaga
     3781 tactcctctc cgaggagatg cgccggggcg agatagagcg tctgaggcga gagatctcgc
     3841 acgccgagaa agaggtcaag cgctcggagg gcaagctggc caacgagaag ttcgtccaga
     3901 acgcccccga gaaggtggtc gccggcgagc gcgagaagct ggacgtgaac gccgggatgc
     3961 tcgagacgtt gagacgccgt ctcgacgagt acctgtagga gagcatatcc tgacgctaac
     4021 cggttttgcc agggtctgcg ccgagttgga tcggcgcagg cacattaccc tcggtttcga
     4081 gaggatcgaa gcactcctgg aggcgatggg ccacccggag cgtgagatcc cccacatcgt
     4141 gcaggtggtc ggaacgaacg gcaagggaac caccgccgtt gccctggccg gcgccctgga
     4201 agcgtcgggc cgtccttccg gcgcctacct ctcaccccac gttctctcct acaccgaacg
     4261 cgtcgtgctc tggggcgagt actcctctga ggacaggttc gcccaggcga tgggggagac
     4321 catcgagcta gccgacaggc acggcatcgg ggtctcgcag ttcgagattc tgactgccgg
     4381 ggcactcagg atgttcgcgg atgcggacgt ctcctgggcc gtcttggagg ccggcctcgg
     4441 ggcgcgccac gacgccacct ccgtcgccgg gcccgaggct gtcgtgctca ccaacgtggc
     4501 cctcgaccac acggagtacc tcggcgatac gatcgaggag atagcagcag agaagctggc
     4561 cagcctgaga ccgggcgcta ccctgtatct cggctccccc gacgaacgcg tggttggcct
     4621 cgcccgcgac acgtgccgca gggttggcgc aaacctcgtc gaggtagccg atcccccgca
     4681 catcaccgcg cctgccggcc tcgccccgta cgctgtcagc aacgtcgagc tgggggtttc
     4741 ggtcgccgaa tccctgatgg gggagagcct ctccggggag accagggaga gcgtagcctc
     4801 ggacgtggtc ggaacactgc cggggcggtt cgaggtacgt gagatgggcg gggtgcccgt
     4861 aatcctggat gggggtcaca acgtggcggg ggtagcggcg gcgctcgcct ccgtgcggtc
     4921 agcttttccg gacaggccct tcggggtcgt cttcggggtc ttgcgggaca aggacgccgc
     4981 gggtatgctc tccgggttcg tccgtggggc ccagatgatc gtgctaacgc ggccagaggg
     5041 tgagagggcc tccgacccgg ggagggtcgg aagcgagcat gaccccctcg atcgtgaagg
     5101 gaggagagcc ctggtggtgc ctgatcctgt agaagcgctg ggtgcggcgg tgaaagcgat
     5161 gagagagacc ggcggtgtgg tgctggtcac cggatcgctc tcgaccatcg cccccgtctc
     5221 ccggtggcta cgtgaagagt aggggtgtcg ggccgttgct ggtgctgctg accctggtcg
     5281 ccgtgacgtc ctttgtgatg ggctacgtcg tgatgataag gtttatccta tgaggctttt
     5341 cctatgatct cgtctatcgt gctgctgcag gacccgtttc agatggtctc cgactttctg
     5401 gcgagcccga tcctcagaat ctccggtcaa ctggtggcgc tcctgttcgt ggt
//
LOCUS       DBNGVD010000071         6243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4932937, whole genome shotgun sequence.
ACCESSION   DBNGVD010000071 DBNGVD010000000
VERSION     DBNGVD010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4932937"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="AC16CM_02015"
     CDS             <1..963
                     /locus_tag="AC16CM_02015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138630.1"
                     /translation="GLFDAMSGLSPSTSGEIADAADLSERYVREWLGAMTVGGIVEHD
                     PQSATYFLPPEHAAFLTREAAPDNMAVTAQFISVMGSVEDEIVEVFREGGGVPYSAYP
                     RFHAVMAENSEQTVVAALVDHILPLVPGLIERLQDGIEVLDVGSGSGKALNLMARTFP
                     RSHFTGYDISEEGVLRAIIEAKRHGSTNVRFEVKDAVTLDETARYDLITTFDAIHDQA
                     HPAAVLENIARALKDGSVYLMQDIAGSSHLQNNMDHPLGPFLYTISTTHCTTVSLAQG
                     GEGLGTMWGEERAREMLAEAGFTNVQTRRLDHDIANTYYVVRKG"
     gene            complement(1008..1418)
                     /locus_tag="AC16CM_02020"
     CDS             complement(1008..1418)
                     /locus_tag="AC16CM_02020"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3138631.1"
                     /translation="MGLDQYLVDAARDLLERRFPGEEGIAAAMYTEDGDVLTSVFFEP
                     EWGSGMLCAETGAICEAEKLGKRVTASVCVSRLSGDDPVLVLTPCGICQERLFHWGPD
                     LEVAVPDPSDPTEWAAKTLREVQPYYWVNAFESQ"
     gene            1671..2156
                     /locus_tag="AC16CM_02025"
     CDS             1671..2156
                     /locus_tag="AC16CM_02025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138632.1"
                     /translation="MQTLIQPTEHAVLVRSASRDDEEGLRRMLSRLSRESVYLRFHAP
                     LPRAPEWAVAMFANSDRSHGESLVAVAGGEVVGHAMYVREGDSAEFAIVVEDRCQARG
                     IGKRLLSALAESAAREGVKCFTGAVLGQNQRMFGLLRSVFERVGYAMEDGAYAVSAPL
                     S"
     gene            3003..4781
                     /locus_tag="AC16CM_02030"
     CDS             3003..4781
                     /locus_tag="AC16CM_02030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3138633.1"
                     /translation="MRFSPSPSRAFDRFDARSLAGSGTRASRPVLLAVDEDERALRGI
                     SRELHKRYGEDYQVSCEKSPRAALQRLRDLEESGEEVALVLADQWMAEMSGTDFLTQA
                     GEISPTAKRALLVGWGDREAVRPVLQAMSLGRIDYYVTKPYGDHDERFHRVISEFLYD
                     WTKDRIPKFEEIRVVGERWSARSFELRDLLGRNGVLHAFHAADSREGRELLARVGHED
                     APLPVVVLFDGQVLTDPSNSDIADACGVNPAWERSDFDLVIVGAGPAGLAAAVYAASE
                     GLDTLIVEGEAVGGQAGTSAMIRNYLGFPSGVSGAELASRAAEQAWLLGATFLFMRRV
                     TGLRRAGREFVVNLSCGQNVTAKAVIVATGASYRHLGVPGLEDLSGSGVFYGAAVSEA
                     RAMAGQEVYVVGAGNSAGQAALHLAKYASRVTLLVRGGSLKTSMSRYLIREIEAARNV
                     AVRLGTRVVDGGGRGRLEHIVLEDSATGLTETVGAAGLFLLIGAEPHASWLPGEIARD
                     ENGYVVTGTEVSYYASPYSGWHVDRLPLPLETSMPGVFAVGDVRRGSVKRVSSAVGEG
                     SMAIQTVHEYLSLSQRDRFPVAAGNP"
     gene            complement(4836..5831)
                     /locus_tag="AC16CM_02035"
     CDS             complement(4836..5831)
                     /locus_tag="AC16CM_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138634.1"
                     /translation="MTEQNGSANGAQNGQPLLEIKDLKKHFQIGRANLKAVDGISLTI
                     DKGETFGLVGESGCGKSTAGRVLVRLYKPSAGEVLFGGENVHSAQGDRGRILNRKMQM
                     IFQDPQASLNPRMVVGDIIAEGIDIHGLASGKQERLDRVNELLETVGLSPEHASRYPH
                     EFSGGQRQRIGVARALAVDPDFIVADEPVSALDVSIQAQVINLMKKLQRDRDLTYLFI
                     SHDLSVVKYISDRVGVMYLGTMVEMAPSDELYSNPLHPYTQALLSAVPIPDPDVELER
                     ERMVLQGDVPSPVNPPSGCRFRTRCPRAVEESAKVDPPWREVSPGHWVQACPCFA"
     gene            complement(5828..>6243)
                     /locus_tag="AC16CM_02040"
     CDS             complement(5828..>6243)
                     /locus_tag="AC16CM_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3138635.1"
                     /translation="QERIGTSIILITHDLGVVASTAHRVAVMYGGKIIETGTLRDIFY
                     NPQMPYTWGLLASIPLPTADRSLELIPIPGSPPSALDPPKGCPFTARCPYAMRICAEE
                     MPEFTRFSDEHRAACWLHHQMAPNVEPPARIGGTR"
BASE COUNT         1085 a         1979 c         2087 g         1092 t
ORIGIN      
        1 ggcctcttcg acgcgatgtc gggtctttcc ccctcgacga gcggggagat cgccgatgcg
       61 gccgacctga gcgaacgcta cgtgcgcgaa tggctcggtg ccatgaccgt cggcggcatc
      121 gtggagcacg acccgcagag cgctacgtac ttccttcccc cggagcacgc cgccttcctc
      181 acgcgcgagg ctgccccaga caacatggcc gtcacggccc agttcatctc ggttatggga
      241 tcggtggaag acgagatcgt cgaggtcttc cgtgagggcg gtggcgtacc ctactccgcc
      301 tacccgcgct tccacgcggt gatggccgag aacagcgagc agaccgtggt ggcggccctg
      361 gtcgaccaca tcctgccgct tgtgcctggc ctgatcgaac gcctgcaaga tggcatcgag
      421 gtccttgacg tgggcagtgg tagcggcaag gccctcaacc tcatggcccg caccttcccc
      481 cgcagccact tcaccgggta cgacatctcc gaggaggggg tgctcagggc cataatagag
      541 gccaagagac acggctcgac caacgtccgc ttcgaggtga aagatgccgt gacgctcgac
      601 gagactgcac gctacgacct catcaccacc ttcgacgcga tccacgacca ggcccatccg
      661 gcggcggtac tggagaacat cgccagggcc ctgaaggatg gcagcgtcta cctgatgcag
      721 gacatcgccg gctccagcca cctgcaaaac aacatggacc atccgctagg accgttcctt
      781 tacaccatct ccaccacaca ctgcaccacg gtctccctgg cgcagggtgg cgaagggctt
      841 ggcacgatgt ggggcgagga gagggccagg gagatgctcg cggaagccgg cttcacgaac
      901 gtgcagacca ggcggctcga ccacgacatc gcgaatacgt actatgtcgt caggaagggg
      961 tagggcttct aacggtcatc cctctgcctc aagctatttg ctgaccgcta ttggctttcg
     1021 aacgcgttca cccagtagta cggctggact tccctcaggg tcttcgcagc ccactctgtc
     1081 gggtccgagg ggtcggggac ggctacctcc aggtcggggc cccagtggaa caggcgttcc
     1141 tggcagatcc cgcacggcgt aaggacaagc acggggtcat cgcccgaaag ccgggagacg
     1201 cacaccgagg ccgtgacccg cttgccaagc ttctccgcct cgcagatggc gccggtctcg
     1261 gcgcagagca tgccgctgcc ccactcgggc tcgaagaaga cgctcgttag cacatcccca
     1321 tcctcggtgt acatcgccgc cgcgatcccc tcctcgccgg gaaagcggcg ctcgagcagg
     1381 tcacgggccg cgtctaccag gtattgatcc aatcccataa gatgctcctc tttcgtcaga
     1441 cggacgtctc actggtgcct ggtacgcatg atagagaagc tccctgtctc acgcttctct
     1501 acgggtaggt ctccggcgtc gctgctgtga tgggcggccg tgagcgatca aagcccttgg
     1561 tccagggagc ttctaggccg aaggtagggg gtgaagtgta gacgcaggtc cgatgtcggt
     1621 attcgtcgct tcgggtctac tttcggtaga ggaaacgaaa ggagaccagg atgcaaactt
     1681 tgatccagcc tacggagcac gcggtcctcg tcaggtccgc gtcgcgagac gacgaggagg
     1741 gcctgcgcag gatgctctca cgcctgtcgc gagagagcgt ctatttacgt ttccacgctc
     1801 cgctgccgcg ggcgccagag tgggctgtcg cgatgttcgc gaactcggac cgcagtcacg
     1861 gtgagtccct ggtcgcagtg gctggcggtg aggtcgtcgg gcacgccatg tacgtgcgag
     1921 agggcgacag cgcggagttc gcaatcgttg tcgaggacag atgccaggcc agggggatcg
     1981 ggaaacggct cctctccgcg ctcgccgaaa gcgccgcgcg tgagggcgta aagtgcttca
     2041 ccggagccgt gctcgggcag aaccagcgca tgttcggtct tctccgctcc gtgttcgaga
     2101 gggtcgggta cgcgatggaa gacggcgcct acgccgtcag cgcaccattg tcgtagcccg
     2161 gcaacctctc agactttggt ctcagaagtt gcccctccca aggtgggtaa caagaaacgt
     2221 tccttcgccc gatgacccgc tccggggtcc ggaccatact tctggcagac agaagagatt
     2281 gacccgaagg aggtccccat gtaccactac aacgatttcg aggcctggca gcatcaccgt
     2341 gacgaactgc tccgggagtc agacatgcgg cgtgtcgccc gacagctacg agcggcgcgc
     2401 tttgaagtga gcgctccccg ggttcgaaac ccgctgccca gtctgatccc cggttttctt
     2461 ctaaggggcc ggaagatggc cggtagctag aaccggccat cttcgtttcg agctgaagcg
     2521 acacccgtgg gaagcctacc gaaacatcct tgcgccgagg agtccatcag gtctaccagg
     2581 accgaagcgc actgcgcacg gaatgcgggg ccgtgggtac tgacgggcgc gcccggggca
     2641 gcgttcctta tcgaagacaa gaggtagccc aggcatcgga cacgagatcc ctcgagacct
     2701 gaggacactg atcgaaccgc tcggcctccg gcgtcggccg acggcccgtc ccatgggact
     2761 ttggtccaga accggtcgtt ctccaaaggt ctgccacgat gttcgttcct tcgcccgagg
     2821 cccaccccgt agtcagggac cacaattacg gcagaggaac ggtaacgaga ggagagggcg
     2881 atggttctca aggatcaaga agtacagtgc gaacgacggt tgaggcagcc cggcaaactc
     2941 ggcctcgcca cccctctagt gatcgtcaga taggcaaacc ccggaccttc gaggagacag
     3001 agatgcggtt ttcacccagc ccgtcgcgcg cgttcgaccg tttcgacgca cggtccctgg
     3061 ccggcagtgg aacgcgagct tcccggccgg tcctgctcgc cgtcgacgag gacgaaaggg
     3121 ccttgcgtgg tatctcgcgc gagctccaca agcgctacgg ggaggactac caggtgtcgt
     3181 gcgagaagtc cccccgggcg gccctgcaac gtctgcggga cctggaggag tcgggggagg
     3241 aggtcgcgct cgtgctcgcc gaccagtgga tggcggagat gagcgggaca gatttcctga
     3301 cgcaggcggg ggagatctcc cccaccgcca agcgggcgct actggtcggg tggggggatc
     3361 gggaggccgt gaggccggtt ctgcaggcca tgtcgctcgg gcgcatcgac tactacgtca
     3421 ccaagcccta cggcgaccac gacgagcggt ttcaccgcgt gatctcagag ttcctctacg
     3481 actggacgaa ggaccggata cccaagttcg aggagataag ggtggtcggt gagcgatggt
     3541 cggcgcgctc cttcgagctg cgcgacctgc tcggacgcaa cggcgtcctg cacgccttcc
     3601 acgccgcgga ttccagggag gggcgggaat tgcttgccag ggtcggacac gaagatgccc
     3661 ctctgcccgt ggtcgtcctg ttcgacgggc aggtgctgac cgacccatcg aactccgaca
     3721 tcgcggacgc ctgcggggtc aaccccgcct gggagagatc cgacttcgac ctcgttatcg
     3781 tgggcgcggg gccagcgggg ctggcggctg cggtctacgc cgcatccgag ggacttgaca
     3841 cccttatcgt ggagggagag gccgttggcg ggcaggcagg aaccagcgcc atgatccgta
     3901 actacctcgg ctttccctct ggcgtcagcg gcgcggagct cgccagccgg gccgcagagc
     3961 aggcctggct actcggcgcg acattcctgt tcatgcgccg cgtcacgggc ctgcgccgtg
     4021 ccggtcgcga gttcgtggtc aacctctcct gcgggcagaa cgttacggcg aaggccgtca
     4081 tcgtcgccac cggtgcctcc taccgacacc tcggggttcc cggcctggaa gacctgagtg
     4141 gctctggcgt gttctacggc gcggcggtct cggaagcgcg ggcgatggcg ggtcaggagg
     4201 tgtacgtggt aggcgcgggc aactcagccg ggcaggccgc gctacacctc gccaaatatg
     4261 cctcccgcgt gacgctgctc gtgcgtggag gttcgctgaa aaccagcatg tcgaggtatc
     4321 tgatccggga gatagaggcg gccaggaacg tcgccgtccg cctcggcacg cgtgtggtgg
     4381 atggcggtgg caggggaaga ttggagcaca tcgtgctcga agattcggcc acgggcctca
     4441 ccgaaaccgt cggcgcagcc gggttgttcc tgctgatcgg cgcggagccc cacgcctcct
     4501 ggctgcccgg ggagatagcc cgcgacgaga acggatacgt cgtcaccggt acggaggtgt
     4561 cctactacgc ttcgccatac agcgggtggc atgtagatcg cctcccgctg ccgctggaga
     4621 cgagcatgcc cggcgtcttc gccgtcgggg acgtgcgccg cgggtccgtg aaacgggtct
     4681 cctcggccgt cggcgagggt tccatggcca tccagacggt ccacgagtac ctgagcctgt
     4741 cacagcgcga caggttcccg gtggctgccg ggaacccgta gatcacaacg acccgcgcgt
     4801 cctggtgggt gagggcccga accccgatct accgatcagg cgaagcaggg gcaggcctgc
     4861 acccagtgtc ccgggctcac ctcgcgccag ggggggtcca ccttggccga ctcctccacg
     4921 gcccgcgggc agcgtgttct gaagcggcag cccgacggcg ggttcacggg ggatggcaca
     4981 tcgccctgga ggaccatacg ctcacgctcc agctccacgt ctgggtcggg gatgggaacg
     5041 gcggagagca gggcctgtgt ataggggtgt agcgggttgc tgtacagctc gtcgcttggg
     5101 gccatctcca ccatcgtgcc gaggtacatc acgccaactc ggtcggagat gtacttgacc
     5161 acggagaggt catgggagat gaacagatag gtcaggtccc tgtccctctg gagctttttc
     5221 atcaggttga tgacctgggc ctgtatcgag acgtcgaggg ctgagacggg ctcgtcggcc
     5281 acgataaagt ccgggtccac ggccagggcc ctggccaccc cgatgcgctg tcgctggccg
     5341 cccgagaact cgtgcgggta gcggctggca tgctcggggc taaggcctac cgtctcgagc
     5401 agttcgttca ccctgtcgag gcgttcttgc ttgcccgagg caaggccatg gatatcgatg
     5461 ccctcggcga tgatgtcgcc gacgaccata cgcgggttca gggaggcctg ggggtcctgg
     5521 aagatcatct gcatcttccg gttcaggatc cgtcccctgt cgccctgcgc cgagtggacg
     5581 ttctcgcccc cgaagagcac ctccccggcg gagggcttgt agagcctgac cagcacccgc
     5641 ccggccgttg acttgccgca gcccgattcg ccgacaaggc cgaacgtctc gcccttgtcg
     5701 atggtgaggg agatcccgtc taccgccttc aggttcgccc ggccgatctg gaagtgcttc
     5761 ttgaggtctt ttatctccag gagcggctgg ccgttctggg ccccgttggc gctaccgttc
     5821 tgttcggtca cctcgtgccc ccgatcctgg ccggtggctc gacgttgggg gccatctggt
     5881 ggtgcagcca gcaggcggcc ctgtgctcgt cagagaagcg tgtgaactcc ggcatctcct
     5941 cggcgcagat ccgcatggcg taggggcaac gggccgtaaa agggcaaccc ttcggcgggt
     6001 cgagtgcgct cggcggcgag ccggggatgg ggatcagctc caggctccgg tccgccgtgg
     6061 ggagcggtat ggaggcgagg agtccccagg tgtagggcat ctgcgggttg tagaagatgt
     6121 cgcgcaacgt tcccgtctcg atgatcttcc cgccgtacat gacggcgacg cggtgggccg
     6181 tgctcgcgac cacgccgagg tcgtgcgtga tcaggatgat cgaggtgcct atgcgctcct
     6241 gaa
//
LOCUS       DBNGVD010000072         3975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_561087, whole genome shotgun sequence.
ACCESSION   DBNGVD010000072 DBNGVD010000000
VERSION     DBNGVD010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_561087"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16CM_02045"
     CDS             complement(<1..518)
                     /locus_tag="AC16CM_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138636.1"
                     /translation="MFENVDFARGWKLYFSISGVLLFAGLVAIALGGLNLGIDFQGGS
                     QFTVANADRQVNEEQIRDALPPELAGDAVITTLGQNGYEIRTPVVGGQEEQGQIQDDL
                     SEASGGEVSVTTVSPAFGEQVRNQALQAVVAALLIIVVFISFRFQFSFALAAMAALMH
                     DILMTVGFYAIVG"
     gene            complement(521..2011)
                     /gene="secD"
                     /locus_tag="AC16CM_02050"
     CDS             complement(521..2011)
                     /gene="secD"
                     /locus_tag="AC16CM_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3138637.1"
                     /translation="MGSRRNNLIVLALVVVLLGVAAYFIFIKEPVTQSTQLGLDLQGG
                     VSAQLQGSQSGGGKVTREEIQQAADLIRQRIDRLGVAEPDVRVQSDDQIVVQIPGIEN
                     PEEVIRVIGSTAQLGFYQVLAADAQPNVPEDEIGATKKDIEETLQDDDAFEKGQTKIL
                     FEETPSVQGRGTDVNGYIVNKTPDMTGDALQQNGATVEFNQQTGKRIVSLELTAEGGR
                     QMSELTQSMLADAAASGEPALLGIALDQDMQSAPQVTEPLGQNISISNDSLPEGLPED
                     EATQLETVLNTGALPVNMTVISETTVGPTLGLSSLKSGLFASLVGFVLVLLLLLFIYR
                     ALGVVADIALLIYAFLLWGLIVIIPVTVTLPGIAGVVLSVGVAADANVVIFERIKEEI
                     RRGKTPRSAIQAGYDKGFRAILDGNVTTLITAFILFALSSGSVRGFAVLLALGVVLSM
                     FTAVVITRALLGLLGDRGIQLSPALMGVTKASMAATRQPQQQARVK"
     gene            complement(2123..3244)
                     /gene="tgt"
                     /locus_tag="AC16CM_02055"
     CDS             complement(2123..3244)
                     /gene="tgt"
                     /locus_tag="AC16CM_02055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3138638.1"
                     /translation="MGSPVDIEIVARDGEARAGILHTPRGPVETPVFMPVGTKGTVKG
                     LTPGDLRSVGAGVVLGNTYHLYLRPGSDLVKEAGGLHRFMAWDGPILTDSGGYQVFSL
                     AETRSISEKGVEFASVYDGSRHEFTPELTTRVQEDLGSDVAMVLDECPPPNAPREYHE
                     DSLARTARWAARCKEAHGKKDQALFGIVQGGLYPDLRERSLGLTVEIGFHGYAVGGLS
                     VGESREAMLETLAITAPRLPADKPRYFMGIGDPVGILQVIALGVDMFDCVLPTRLARH
                     GAALTPDGRLNLKNARFRTDFGPLDPECSCEACTHYSRAYLAHLVRENELLGHRLITL
                     HNVRFTVDLCRRARREIRVGRFGAWSRAWISRFEASRTP"
     gene            complement(3241..>3975)
                     /locus_tag="AC16CM_02060"
     CDS             complement(3241..>3975)
                     /locus_tag="AC16CM_02060"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR3138639.1"
                     /translation="AWEVLARPSKRLRPGLVLTVEGQSLTLSKSLGDGHWAVSGSDLP
                     GLLRRRGRMPLPPYISPTPETEERYQTVYARNEGSAAAPTAGFHFTEQVLEDAERAGA
                     GIARITLHVGVGTFMPVRAERLEEHTMHAEHYIVPRDAAETVLGARRVLAAGTTVART
                     LETWAATGEAEGESELFVYPGYRWLAVDALLTNFHLPRSTLLAMVMSFGGEGLVRRAY
                     DIAVRERYRFYSFGDAMLLLNGGRRL"
BASE COUNT          704 a         1337 c         1230 g          704 t
ORIGIN      
        1 cccacgatgg cgtagaaacc aaccgtcatc aggatgtcgt gcatgagcgc cgccatcgcc
       61 gccagggcga aggagaactg gaagcggaag ctgataaaga ctacgatgat gagcaacgcg
      121 gccaccacgg cctggagggc ctggtttcta acctgctcgc cgaaggccgg gctcacggtc
      181 gtaacgctaa cctccccacc tgaggcctcg ctgaggtcat cctggatctg gccctgttcc
      241 tcctggccac ccaccaccgg agtcctgatc tcgtagccgt tctggcccag ggtcgtgatg
      301 acggcatccc cggccaactc gggcggcagc gcatcgcgta tctgctcctc gttgacctgg
      361 cggtcggcat tggccacggt gaactgcgaa ccaccctgga agtctatgcc caggttcagg
      421 cccccgagcg ctatggcaac cagccccgcg aacagcaaga cgcctgagat cgagaagtac
      481 agtttccaac cgcgagcgaa atcgacgttc tcgaacacga ctacttcacc ctcgcctgct
      541 gctgtggctg ccgcgtcgcc gccatgctcg ccttcgtgac gcccatcagc gccggcgaaa
      601 gttggatgcc gcgatcaccg aggaggccta gcagggcgcg cgtgataacc accgccgtga
      661 acatcgagag cacgacgccg agggcgagca agaccgcgaa cccgcgaacc gagccgctgg
      721 agagcgcgaa caggatgaac gcggttatca gggtggtgac gttaccgtcg aggatggccc
      781 tgaagccctt gtcgtagccg gcctggatgg cgctccgagg cgtcttcccg cgtctgatct
      841 cctccttgat ccgctcgaag ataaccacgt ttgcgtcggc cgccaccccg acgctcagga
      901 cgacgccagc gattcctggc agcgtcaccg ttacggggat gatcacgatc agcccccaca
      961 acaagaacgc gtagatgagc aaagcgatgt ctgccaccac acccagcgcc cggtagataa
     1021 agagcagcaa gaggagcacg agcacgaagc ccacgagcga ggcgaagagg ccgctcttga
     1081 gggagctgag gccgagcgtc ggccctacgg tcgtctcgga gatcacggtc atgttcacag
     1141 gcaaggcgcc ggtgttgagc acggtttcga gctgggttgc ctcgtcctcc ggaagaccct
     1201 caggcaggct atcgtttgag atcgagatgt tctgtcccag cggctccgta acctgtgggg
     1261 cgctctgcat atcctggtcg agcgctatgc cgagcagggc gggctctccg ctcgctgcgg
     1321 cgtcggcgag catgctctgg gtaagctcgc tcatctggcg cccgccctca gcggttagct
     1381 cgaggctgac tatgcgcttg cccgtctgct ggttgaactc gacggtggca ccgttctgtt
     1441 gcagggcgtc gcccgtcatg tcgggggtct tgttgactat gtacccgttg acatccgtgc
     1501 cccgaccctg caccgagggg gtctcctcga agaggatctt ggtctgcccc ttctcgaagg
     1561 cgtcgtcatc ctgcagggtc tcttctatgt ccttcttggt ggcgccgatc tcgtcttcgg
     1621 ggacgttcgg ctgggcgtct gccgccagaa cctggtagaa gccaagctgg gccgtgctcc
     1681 cgatgacgcg gatcacttcc tccgggttct cgataccggg gatctggacc acgatctggt
     1741 cgtcggattg cacccgcacg tcgggctcgg cgacgcccag gcggtctatg cgctgccgga
     1801 tcaggtcagc cgcttgctgt atctcctcac gcgtgacctt gcccccaccg ctctgagagc
     1861 cctgcaactg ggcgctgacg ccaccctgca ggtcgaggcc aagctgcgtc gactgggtga
     1921 cgggttcttt gatgaagatg aagtaggcgg ccaccccgag aaggacgacc accaacgcca
     1981 gcacgatcag gttgttacga cggctaccca taagatcgga gggtcattct agctcatctg
     2041 tgcaggaatt gtagaccggg gcaagtatcc tctgggccaa tttctctcca cctgaagccg
     2101 taaaccgtac cggagtcgag gatcacggcg tcctgcttgc ctcaaagcgg gagatccagg
     2161 cccgagacca cgccccgaac cgcccgactc gtatctcgcg ccgcgcccgc cggcagaggt
     2221 ccacggtgaa ccgaacgttg tgaagcgtta tgagcctgtg gcccagcaac tcgttctccc
     2281 ggacgagatg agccaggtac gccctgctgt agtgcgtgca ggcttcgcag gagcactcgg
     2341 ggtcgagcgg cccaaaatct gtgcggaacc tcgcgttctt gaggttgagc cggccatcgg
     2401 gggtcagggc cgccccgtgg cgggctagcc gcgtgggaag cacgcagtcg aacatgtcca
     2461 cgccgagcgc gatgacctgc aagatcccga ccggatcacc gatacccata aagtacctgg
     2521 gcttgtcggc gggaagccgg ggggccgtga tggccagggt ctcgagcatc gcctcgcgcg
     2581 actcgccgac cgacaggccg ccaacggcgt agccgtggaa cccgatctct accgtaagcc
     2641 ccaggctccg ttccctcaga tcgggataga gcccgccctg cacgatgccg aagagcgcct
     2701 ggtctttctt gccatgcgcc tccttgcaac gcgccgccca gcgcgccgtt ctggccaggg
     2761 aatcctcgtg gtactcgcgg ggtgcattgg gtggcgggca ctcgtccagc accatggcca
     2821 catccgatcc caggtcctcc tgcaccctgg tcgtcagctc gggcgtgaac tcgtggcgcg
     2881 agccgtcgta gaccgaggca aactccacgc ccttctccga gatgctgcgc gtctccgcca
     2941 gcgagaacac ctgatatccc ccggagtccg tgagtatggg gccgtcccag gccatgaagc
     3001 ggtggagccc accggcctcc tttacgagat ccgaacccgg tctcagatac aggtgatagg
     3061 tgttccccag cactacgccg gcccctacgg atctcagatc tccgggtgtg agacctttga
     3121 ccgtgccctt ggtccccacg ggcatgaaga caggtgtctc cacggggccg cgcggcgtat
     3181 gcaggatgcc cgctcgcgcc tcgccatcac gggcgacgat ctcgatgtcg acgggcgaac
     3241 ctatagtcgc cgaccaccat tgaggagcag catggcgtcg ccaaacgagt agaagcggta
     3301 gcgctcgcgc accgcgatat cgtacgcccg ccgcacgaga ccctctcccc cgaagctcat
     3361 caccatggcc agtagcgtgg agcgcggtag gtggaagttg gtaagtagcg cgtccacggc
     3421 cagccaccgg tagccgggat atacgaacag ctccgactca ccttccgcct cgccagtcgc
     3481 tgcccacgtc tcgagcgtac gggccacggt cgtgcccgcc gcgaggaccc gccgggcgcc
     3541 cagtacggtc tccgcagcgt cccgaggcac gatgtagtgt tccgcgtgca tggtgtgctc
     3601 ttccagcctc tcggccctca cgggcatgaa cgtacctacc ccgacgtgca gcgtgatcct
     3661 ggcgatgcca gcccctgccc gctcggcatc ttccaggacc tgctctgtaa aatggaatcc
     3721 ggccgtcggc gcagcggccg aaccctcgtt tcgggcgtag accgtctggt aacgctcctc
     3781 ggtctccggc gtggggctta tgtacggcgg caatggcatc ctgcccctgc gacgcaacag
     3841 gccgggtaga tcggagcccg acacggccca gtgtccgtcc ccgaggctct tcgacagcgt
     3901 cagcgattgg ccctcgacgg ttagcacgag ccccggcctc agccgcttgc tcggtctggc
     3961 cagtacctcc catgc
//
LOCUS       DBNGVD010000073         5240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3659315, whole genome shotgun sequence.
ACCESSION   DBNGVD010000073 DBNGVD010000000
VERSION     DBNGVD010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3659315"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="AC16CM_02065"
     CDS             <1..396
                     /locus_tag="AC16CM_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HIR3138640.1"
                     /translation="VNMWERIFEEKPMETWLAMNKWVNDGPPFPGATFKQWISDFYQQ
                     NKLAKGEIVLRGRRVDLSNIKVPLLNIAGKKDHICTLPQAEATMNLVGSEDKEFFVLD
                     AGHVGLMTGRGAKRGLWPKVSDWLGTRSG"
     gene            complement(436..1590)
                     /locus_tag="AC16CM_02070"
     CDS             complement(436..1590)
                     /locus_tag="AC16CM_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138641.1"
                     /translation="MKGAVRLGLAGFAATAVAYGPARTGYGLFVPEFRGDFGISTSAA
                     GAIGSGLQGGFLLALVATAVLVGRLGPRVLIAAGGVSATVGMLLVALAPDGVTLAVGV
                     CVAGMTAGFCWSPYNDLAGWNVRAGLRSRVLSAVSTGTTMGIAVSGLVALAAVAGELP
                     WRAVWLAFVAGAVLSAVLNALLLMGMSDGSGSVGQRGGGWIFRAGSSPLFVVASSFGV
                     VNAFYWSFNADIVSSAGLPLPNAGPVLYAVLGVAGFAGLFTGDLVDRFGLGVTLRFTL
                     LCLGVGSMLLGIAPGSVGVVGVAASLYGAGVMLMSALLSLWSTTVFSERPSQGFTAAL
                     LFFGVGGVVGPAVLGAVGGAFGLGTAFVVAAIVILLNLPIPVQNKSQDGE"
     gene            1842..2429
                     /locus_tag="AC16CM_02075"
     CDS             1842..2429
                     /locus_tag="AC16CM_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HIR3138642.1"
                     /translation="MQSMGWLGTGWNNPKPAQGALGLVQDFVNTRNYLQGGDLLGGVG
                     EADRRLAERGLLGAGEGVGELGRQRLVAFREALRGLLLAHNGVGGPGVEGLNELVADV
                     SLGVSFSSDGRPVLGPTGGEDPAACILARLLAEVFRAEAEGKWGRLKACGNPDCLWIF
                     YDSSKNGLGRWCDMQLCGARHKMRRYRERQSSGEA"
     gene            2564..2959
                     /locus_tag="AC16CM_02080"
     CDS             2564..2959
                     /locus_tag="AC16CM_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3138643.1"
                     /translation="MGYRTQDVTALGQDWAAAELRGDTAFLAEVLAEDFVGVGPRGFM
                     LTRGQWLSRHEAGSLTYESFQLDELQTRTYGDAAITVCRQSARAVYQDENGRFELDDP
                     FRATLVFVRQEGRWKLAGLQLSPIQGRPS"
     gene            3041..3382
                     /locus_tag="AC16CM_02085"
     CDS             3041..3382
                     /locus_tag="AC16CM_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138644.1"
                     /translation="MVYVIHQDELPHSETAHRFEGYLNGGAGVSFFLVDSERSGGPDL
                     HTHPYAEVFVVQEGEVTFTAGDEVIEAGTGQIVVVPAGVPHKYVNSGTGRTRHVDIHA
                     SGSMETEWLES"
     gene            3379..4263
                     /locus_tag="AC16CM_02090"
     CDS             3379..4263
                     /locus_tag="AC16CM_02090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3138645.1"
                     /translation="MRLGLQLNSFDWRGGPGQFASNLVDIGRAAEEAGFDRIGVADHL
                     WQHPIMGGPEANEPECYATLAYLAANTERVGLTAMVSGIHFRHPAVLVKAVTTLDVLS
                     GGRAGLGVGSGHYEEEASGLGIPFPSQKERFEMLEETLQIAHRMWSGERGDERPFEGE
                     HYRLGRPLNLPQSISRPHPPIMVAGDGERKTLRLVARYADACSLRPGPQVPEKLDALR
                     RHCEEAGTDYRSIEKTCAFLFDIGENREKAEELVRQLRWLAGMGIETVIGAVPRVDEI
                     RQLGIIGEQVIPAVADVP"
     gene            4340..4798
                     /locus_tag="AC16CM_02095"
     CDS             4340..4798
                     /locus_tag="AC16CM_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138646.1"
                     /translation="MTQHTTIHRRNGVEKTIGVLSAVYAATFFFGALLHLGVGIPLGF
                     AMIEEPVILPATIVEGLCGTALAVAAFAVFARRAGAWTVATAAHAFALGGVSLGIVAL
                     AVGAGPSTELNTAYHRVMVVALVAVLGLLLSPTGRNALHRATSPSVIQSR"
     gene            4824..>5240
                     /locus_tag="AC16CM_02100"
     CDS             4824..>5240
                     /locus_tag="AC16CM_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138647.1"
                     /translation="MTESVKGEERPLGGKNSRLPRGGRLLGPLFGMVFLIYPVKTVLT
                     SAPTPTRVALALGGAALFAGVFLWLMWTQEPLRSPSAGSSEVWKRRATVAFLILLVIS
                     LNLALGSEWRVLFFHLNVVAGIMLLTTDAYVTIACLA"
BASE COUNT         1039 a         1650 c         1729 g          822 t
ORIGIN      
        1 gtcaacatgt gggagcgcat cttcgaggag aagccgatgg agacctggct cgccatgaac
       61 aagtgggtca acgacggtcc ccccttcccc ggcgccacct tcaagcaatg gatctcggat
      121 ttctaccagc agaacaagct ggccaagggc gagatcgtgc tccgtggccg ccgcgtggac
      181 ctctcgaaca taaaggtccc gctgctgaac atcgctggca agaaggacca tatctgtacc
      241 ctcccgcagg ccgaggccac catgaacctg gtcggcagcg aggacaagga gttcttcgtc
      301 ctcgacgccg gacacgtcgg cctcatgacc ggccgcggcg ccaagcgggg attgtggccg
      361 aaggtcagcg actggctcgg tacccgctcg ggctagagag ccaacggcta gagaccggaa
      421 aagccgggac gggggtcact ccccgtcctg gcttttgttc tgcaccggga tgggcaggtt
      481 gaggaggatc acgatggccg ccaccacgaa ggccgttcct agcccgaaag cgccgcccac
      541 cgccccgagt acggccggcc ccacaacgcc cccgacaccg aagaatagta gggcggcggt
      601 gaagccctgc gacggacgtt ccgagaagac cgtcgtactc cagagagaca acagcgcgct
      661 cattagcatc accccggccc cgtagagcga ggccgcaacc cccaccacgc ccacggaccc
      721 gggggcgatc ccgagcagca tggaacccac gccaaggcag agaagggtga agcggagggt
      781 tacgcccagc ccgaagcggt cgacgagatc tccggtaaag aggccagcga accccgccac
      841 gccgagcacg gcgtagagaa ccggtccggc gttcgggagc ggcaacccgg ccgaggagac
      901 gatgtcggcg ttgaaagacc agtagaaggc gttgaccacc ccgaacgagg aggccacgac
      961 gaacaggggg ctagacccgg cgcggaagat ccaaccccct cccctctggc ccaccgaccc
     1021 actcccatca gacatcccca tcagcagcag ggcgttcagg acggcggaga gaaccgcgcc
     1081 ggcgacgaac gcgagccaga ccgcccgcca gggcagctcc cccgcgacgg cagcaagcgc
     1141 gacgagaccg gagaccgcga tacccatcgt ggtgcccgtg ctaaccgccg agagcacgcg
     1201 cgaccgcaac cccgcccgca cgttccaccc agccagatcg ttgtacggag accagcaaaa
     1261 accggcggtc atccccgcca cgcaaacccc taccgcgagc gtcactccgt ccggggcgag
     1321 ggccaccagc agcatcccga cggtggccga aaccccacca gcagcgatga gcacccgcgg
     1381 cccgagacgg ccaacgagca ccgccgtggc caccagcgcc agcaggaacc cgccctgcag
     1441 cccgctcccg atggcgccgg cagcagaggt cgagatcccg aagtctcccc tgaactcggg
     1501 gacgaataga ccgtagcccg tgcgcgccgg cccgtaggca acggcggtcg cagcgaaacc
     1561 cgccaacccc agacgaaccg cacccttcat accgaacccg cccgtacgtt catgctccca
     1621 ctcccgcgga agcgggaacc cacgggttgc gtaagggatg gacccgcact cgccggtata
     1681 gccccctggc aaatccagat ctcacccgaa tagacacccc cggatgctcg taccagaaaa
     1741 tacacgcctc cttccctcac aatactacag ttgttgttac tggtataaga ttataccggt
     1801 aacaagttac ctctaaacac gatagaaggt aaaattcagg gatgcagagc atgggatggt
     1861 tggggacggg atggaacaat ccgaaacctg cgcagggtgc gttgggcctg gtgcaggatt
     1921 tcgtaaacac gagaaactac ttgcaggggg gcgacctcct gggaggtgtg ggggaggcgg
     1981 accgcaggct cgccgagcgc gggttgcttg gggcaggtga gggcgttggg gagttgggca
     2041 gacaacgtct ggtcgccttc agggaggctt tgcgagggtt gctgctggcg cacaacggcg
     2101 tgggcgggcc cggggtggag ggtttgaacg agctcgtagc cgacgtgtcc ctgggggtct
     2161 cgttcagttc ggacggacgg cccgtcctcg ggcccacggg tggcgaagat ccagccgcgt
     2221 gcatcctggc gcggttgctc gcggaggtct tcagagcgga ggccgaggga aagtggggaa
     2281 ggctcaaggc ctgcggtaat ccggactgcc tctggatctt ctacgatagc tccaagaacg
     2341 gactggggcg ctggtgcgac atgcagctct gcggggccag gcacaagatg cgccgctacc
     2401 gcgagcgaca gagctcgggg gaggcgtagg cgaccgccgt tgccggctag acttgcgttc
     2461 cgtctcatga catccgtcat atagatcaca cgagcatctt cactaccggg cacgaattca
     2521 tccccataca ctcctctcta ccacgagata aaggagaccg ttcatgggct atagaacaca
     2581 ggacgtaaca gccctcggcc aggattgggc cgcggcggag ttgcgcggag acaccgcgtt
     2641 tctcgcggag gtcctggccg aagacttcgt gggggtgggg ccgcggggct tcatgctgac
     2701 caggggacag tggctctcgc gccacgaggc gggaagcctc acctacgagt ctttccagct
     2761 ggacgagctg cagacgagga cctacggtga tgcggcgata acggtatgcc ggcagagcgc
     2821 gagggcggtc taccaggacg agaacggccg attcgaactc gacgatccgt ttcgcgccac
     2881 gctcgtgttc gtgaggcaag agggacgctg gaaactggcc ggtctgcagt tgagccctat
     2941 ccagggtagg ccttcttagg ctttcagcta ttagccatca gctttcagct attggctatc
     3001 ggcgtggttt gcgaaaggcc ttagtgaaca ggaggagaac atggtctacg tcattcacca
     3061 agacgagctg ccgcacagcg agacggcgca caggttcgag gggtacctca acggcggcgc
     3121 tggggtctcg ttcttcctgg tcgactcgga acggagcgga gggcctgatc tgcacaccca
     3181 tccttatgcg gaggtgttcg tggttcagga gggtgaggtt accttcaccg cgggcgacga
     3241 ggtcatcgag gcggggaccg ggcagatcgt ggtcgtgccg gccggggtgc cacacaagta
     3301 cgtcaactcc ggaaccggaa ggacgaggca cgtcgacatc cacgccagcg ggagcatgga
     3361 gacggagtgg ttggaatcat gaggctcggg ctgcaactaa acagcttcga ctggagaggc
     3421 ggacccgggc aattcgcctc gaacctggtg gacatcggac gcgccgccga agaggcaggc
     3481 ttcgatcgta tcggggtggc ggatcatctc tggcagcacc cgatcatggg cggccccgag
     3541 gcgaacgagc cggaatgcta cgcgacgctg gcctacctgg cggccaacac cgagcgggtt
     3601 gggctcacgg cgatggtgtc ggggatacac ttccgtcacc cggcggtgct cgttaaggcg
     3661 gttaccacgt tggacgtgct ctccggcgga cgcgccggcc tcggcgtcgg ctcgggccac
     3721 tacgaggagg aggcgagcgg gctcggcatc ccctttcctt cccagaagga acgcttcgag
     3781 atgctggagg agacgctcca gatagcccac aggatgtgga gcggcgagcg gggagacgag
     3841 cgtcccttcg agggggagca ctacaggctc ggacgccccc tgaacctgcc gcagagcatc
     3901 agcaggccgc acccaccgat catggtcgcg ggcgatggtg agagaaagac actacgactg
     3961 gtcgcccgct acgccgacgc ctgcagcctt cgccccgggc cacaggtccc cgaaaagctc
     4021 gacgcgctgc gccggcactg cgaagaggcg ggcacggact accgatccat agagaaaacc
     4081 tgcgccttcc tcttcgacat cggcgagaac agagagaagg ccgaggaact cgtaagacaa
     4141 ttacgctggc tagctgggat gggaatagag accgtcatcg gcgccgttcc cagggtggat
     4201 gagatccggc aactcggaat catcggggag caggtcatcc ccgccgtagc cgacgttcca
     4261 tgaacgacca cccatgtagg ggccggcttg aaacccaccc ggttcgccaa ccgacctgcg
     4321 caatcgaaag ggagagacta tgacccagca caccaccatt catcgacgaa acggtgtaga
     4381 gaagacgatc ggcgttctgt ctgctgtcta cgccgccacg tttttcttcg gggcgctcct
     4441 gcacctgggg gtggggattc cgctcgggtt cgcgatgatc gaagagccgg tgatcctacc
     4501 ggcgactatc gtcgagggcc tgtgcggaac ggcgctggcg gtcgcagcct tcgccgtgtt
     4561 cgcccgtaga gccggggcgt ggacggtggc tacggcggcc cacgccttcg ccctgggtgg
     4621 cgtctctctc ggaatcgttg cgctggccgt tggcgcggga ccttcgaccg agctcaacac
     4681 cgcctaccac agggtcatgg tggtggccct tgtggccgta cttggcttgc tattgtcgcc
     4741 cacgggtagg aacgcgctcc atcgcgccac gtcgccttcg gtaatacaat cgcgctgagc
     4801 tcctcgaagg ggtttgggat cctatgaccg agagcgtaaa gggcgaagaa cgcccgttgg
     4861 gtggcaagaa ctctcgccta ccgcggggcg ggcggttgct ggggccgctg ttcgggatgg
     4921 tcttcctgat ctacccggta aagacggtgc taacttctgc tcccacaccc acgcgggtcg
     4981 cgctcgccct aggtggggcg gcgctcttcg caggcgtatt cctgtggctg atgtggaccc
     5041 aggagccgct acggtcacct tcggcagggt cttcggaggt ctggaagcgt cgggcgacgg
     5101 tggcgttcct catcctgctg gtcatctcgc tgaacctcgc gctcggtagc gagtggcgcg
     5161 tgctgttctt tcacctgaac gtcgtcgccg ggatcatgct cctgaccaca gacgcctacg
     5221 tgacgatcgc gtgccttgcg
//
LOCUS       DBNGVD010000074        18823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3294451, whole genome shotgun sequence.
ACCESSION   DBNGVD010000074 DBNGVD010000000
VERSION     DBNGVD010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18823
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3294451"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..828
                     /locus_tag="AC16CM_02105"
     CDS             460..828
                     /locus_tag="AC16CM_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138648.1"
                     /translation="MHRAGMFLTLMGVLVLVFAGVATAAIINGNDRPNTLIGTPRHDV
                     IHGYGGGDLIRGRGGEDDMFGGRGGDYIRAVDRREDDVSCGRGNDKVRANPGDEVNRG
                     NCERIIRAGIRVDDNDGGDD"
     gene            1339..3000
                     /locus_tag="AC16CM_02110"
     CDS             1339..3000
                     /locus_tag="AC16CM_02110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3138649.1"
                     /translation="MARWRLLEPDSSVVSELSRAAGLDPLCARVLANRGVTPEGAEDF
                     LSPSLSAISTPDGDAWGVAARRVARAIRDGERIGVFGDYDTDGITSAAVLFLALSHYT
                     QNITVGLPTRQTGYGLLEPYVRNLFAEGVDLLITADCGISNRAEVALAGELGMEVIVT
                     DHHIPPENPPDTAVAVLDPKLWDPEDPLAGVGVAWKFAWAVVRELGDAEGRKLLGRLL
                     DLVSVGTVVDIAPLVGDNRALARMGLKHINRGLAAGGARPGIAALVKVAGVRGDLDES
                     DLGWKLGPRLNSIGRIKNPRPALDLLLTQDRREAFRIASELNQLNSERQRRTRFAVER
                     AMEEVDPDQDFKVVVSEETGGIAGLIAGRVAGATGRPAAILTRRADDSYGGSARAGET
                     EVDLYGALYSVRHLMGEWGGHRKAAGLSVKPGRFDAFVAGVNEAVRAQAEENPEIFVP
                     AIEIDAEIPLSKVSNGLLEWHERLAPFGSGNYRPVFVTEGLRIGGSRKIWEGMNLVDL
                     DGVKAKMACDPGELPTTAFDAVYTISRSRYSGRAELEIVDWRTGS"
     gene            2997..3950
                     /locus_tag="AC16CM_02115"
     CDS             2997..3950
                     /locus_tag="AC16CM_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3138650.1"
                     /translation="MNAIGVDVGGTKIAAAVVTPHGRILDEVRYPTPQSLETLIENIA
                     RAVREVGDRYEAGGVCLAVPGLILGSENKVIFSPNLGAIEGVPLKDELEPRIGLPLTI
                     ENDGNAAAWGEFRFGVGSEADHLVFVTLGTGIGGGVITHGVLMRGAQGSGGELGHVTI
                     QEGGPRCACGNYGCLEALASGTAIARRGRERAIENPGSALGSLATRRQILGEDVTRLA
                     REGDAVALSVLEETGRWLGIGLASFVNIFNPEVVAVGGGAMEAGELILKAARREIMDR
                     ARPPSRDLAEVQVATLGPKSGVLGAAALARDASSGEYLLGT"
     gene            3954..4763
                     /gene="rsmI"
                     /locus_tag="AC16CM_02120"
     CDS             3954..4763
                     /gene="rsmI"
                     /locus_tag="AC16CM_02120"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3138651.1"
                     /translation="MPLSVIPTPIGNLEDITLRALRHLREADLVACEDTRRTGRLLAH
                     YEIKQNLFAYHEHNEERLAPELAKRARTENIALVTDAGTPLVSDPGYRLVRACIEAGV
                     TVEVLPGPSSLTTALAVSGLPTDSFVFLGFLPRKGRIRTETLERIQREETTFVLFESP
                     NRLARTLRDLPPGVPVAVCRELTKLHEEVFRGTAAEAAEHFSGKVKGEIVVVVRGDDE
                     RGSATLEEAVEIARGHVSEGESPSRAAALAARESGHRKAEIYAGLVNSRSE"
     gene            4920..5678
                     /locus_tag="AC16CM_02125"
     CDS             4920..5678
                     /locus_tag="AC16CM_02125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3138652.1"
                     /translation="MLVDSHTHLLRLEVSPEEAVKSAADVGVGAVVNIGTEVEDSQRG
                     AELAAVLPNVYSTAGIHPHNAGTYTAADLEALAELSESPGIVALGEVGLDYYRSEWSE
                     EVQRALFEDVISLANDTRLPLVIHTREAFADTMACLGSARVPIVLHCFEGGDREVREA
                     TERGYYIGLAGNVTYKGSETARVLRLLDEERILVETDAPYLSPQPARGRKNVPENVVY
                     TAGFVAERLGMDDDDFATLTTRNARNLFGLPLEV"
     gene            5685..6461
                     /gene="rsmA"
                     /locus_tag="AC16CM_02130"
     CDS             5685..6461
                     /gene="rsmA"
                     /locus_tag="AC16CM_02130"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3138653.1"
                     /translation="MSEQGPPKKRFGQHFLKDPNTARIVASGVTKDDVVLEIGPGRGF
                     LTSFLAERAGLVHAIELDPDVLPALREAARGRNNVIVYKGDALRFDYSALDPEPTRLV
                     ANLPYNIASPLVLALLEEVDSIRTLRFMVQLEVARRMAADRGSKDYAAYAVLAQLLAS
                     VRVLHRVPPTVFDPPPRVHSAVVEMDRKSKPDGYQGIKRLVLGAFRSRRKRLVNNLPE
                     PVRREAPRVLQSLGYGPDARAEELAPEDFVALYRALSWAL"
     gene            6461..7333
                     /gene="ispE"
                     /locus_tag="AC16CM_02135"
     CDS             6461..7333
                     /gene="ispE"
                     /locus_tag="AC16CM_02135"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3138654.1"
                     /translation="MREVRLRAFAKVNFALDVLGLRVDGYHDIATVMQSISLSDEVDI
                     ERIAEGFEFDFEPEGEEMGPPERNTAYVAWKSLQRLTGLALPVRVSLRKWIPVGAGLG
                     GGSADAAAVLVGLNELFDLGLGMEGLREVGARVGADVPFCISGGTALGEGVGERLTQL
                     PSPPAQRLVLAKPHRSADTGEIYRAHDAMGAKSALCVEPVVSALRSRSLRDLAAAVGN
                     DLVSVTRELVPEVKTLQRDLLRCGALGATMSGSGTAVYGIFDDEGAAERAAKAVDVPF
                     AGVYQPVPHGVEFV"
     gene            7708..7778
                     /locus_tag="AC16CM_02140"
     tRNA            7708..7778
                     /locus_tag="AC16CM_02140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7740..7742,aa:Gln,seq:ttg)
     gene            7819..9222
                     /gene="glmU"
                     /locus_tag="AC16CM_02145"
     CDS             7819..9222
                     /gene="glmU"
                     /locus_tag="AC16CM_02145"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3138655.1"
                     /translation="MSQSSPIFAVVLAAGKGTRMKSNRAKVLHTLCGIPMVNYVIGAT
                     RPLLPEGTLVVVGHQADLVEDVLPDDATAVLQEEQLGTGDAVRVALSAIPEDQEGTLL
                     VVNGDGPLISDRTLGELVERHRSAGVGATVLVAEPDDPSGLGRVVEDAGVVRIVEEKD
                     ATEAERRIGLVNLGLYAFRIPEIREAVGGLSTENMSGELYLTDVLETIGRRSRAVTYR
                     LKDMEESHLVNDRAQLAVAEEILRRRILDAHMREGVTVRDPASTHIDATVEIGRDTVI
                     LPGTLLRGGTKVGTDCVIGPNTDLQDTLVSEGALVEHSVGRGAEVGAGATVGPYSFLR
                     PGTKLGVGSKVGAYCELKDSRVGEGSKVPHLSYIGDAEIGAGVNVGAGTITANYDGVN
                     KNRTTLGDGVFTGVNTALIAPVEVGEDAYTGAGAVINKNVPAGKLAVGSPARAIAESP
                     QKRRKRQDGPQEKETGG"
     gene            9233..10225
                     /locus_tag="AC16CM_02150"
     CDS             9233..10225
                     /locus_tag="AC16CM_02150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3138656.1"
                     /translation="MQNGYLTQTTEKRLMIVSGDGYPTLAESIADRLDMELTGVELTE
                     FANGELYARYLESVRGADMFIVQSLCEPVNDNLMQLLIMIDAAKRASAKRIVAVIPWY
                     AYSRQDRKTKPREPITARLVANMIAAAGAERVMTMDLHTGQIEGYFSFPVDHLTAMHT
                     FVDYFVGQGFRGAEDGVVVAPDTGEVKVAKQLAGHLGLPWAIVNKMRDRPGHSEVTHV
                     IGDVAGKRVIMIDDVIATGGTIANAAQRLVDEGATEVSAAATHGEFSGDAYRKLDESP
                     LKEIVVTNTLPLNENQPQAKIKTLNIAPILASTIKNVFTDESVSEVFMGENQLF"
     gene            10243..10962
                     /locus_tag="AC16CM_02155"
     CDS             10243..10962
                     /locus_tag="AC16CM_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3138657.1"
                     /translation="MADNIDLQAQERYERGKNAARRLRASGMTPAVLYGEGNEAGGST
                     ALAIPTRIVDYTLHHYGDNALYNIAIDGNTSTARVVDAHRDPVSGLLVHVDFAPVNMT
                     ERIEVTVPLSVTGDAPGVEEGGVLQQVAYEIQVESLPGDIPQEITLDVSELGMNENLT
                     LGDLTLPDGVALLSEPEEVAVTVTAPTEISEEEMEAAGIVEEPTDEEEAAGGEEAAEG
                     EETPEESAEEGEGPAGQNVQA"
     gene            10964..11548
                     /gene="pth"
                     /locus_tag="AC16CM_02160"
     CDS             10964..11548
                     /gene="pth"
                     /locus_tag="AC16CM_02160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3138658.1"
                     /translation="MVRSFFSGEAKGDGGLLESPVVVGLGNPGRSYERTRHNVGYLVA
                     DELATRHAGSWRKRKKAEAAPITLGLVEATLLKPTTFMNNSGSAVSGYRPEDLVVVHD
                     DLDLETGTVRVKVGGGAGGHNGLRSIIGRLGNDFVRVRVGIGRPPVGVGVTDYVLSRM
                     DSGVKEAIPAAADAVEFLLEQGPEAAMNRFNVRG"
     gene            11635..12273
                     /locus_tag="AC16CM_02165"
     CDS             11635..12273
                     /locus_tag="AC16CM_02165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138659.1"
                     /translation="MVSRLLHGGTAVARSDGSLHDLFPIVVSAAEGEALRDWVLRERA
                     TSTVEVGLGYGISALHICEGLLQNSGPSPHHVAIDPYQATRFSDCGLQFLDEAGVREM
                     VELHTEDSQISLPRFLDGDRTFDLAFIDGNHRFERVFVDLFYLGRLVRPGGIIFLDDY
                     QLPGIARAASFFVSNLGWTTEEISPPDDLHQWAALRTSAVPDERSFDYFVDF"
     gene            12511..13689
                     /locus_tag="AC16CM_02170"
     CDS             12511..13689
                     /locus_tag="AC16CM_02170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3138660.1"
                     /translation="MRKTSLLLASIAFAVMLAGGAALAASSEAGNPGNAEARDAVLDA
                     SMKALVERRGGPPGAIAVVQRGNDREVHAFGVSNLKRDLPMRADARMRQASTSKAFNG
                     AVALSLVDKGEMSLNDTIGEYLPGLPKSWHEVTLRQLLDHTSGLPNFTEDPGYLRALS
                     ASPENAPTPRKLLSYVEDEPLNFEPGSDYRYSNSDNVAVGLMVRVATGDTYEEQLQEQ
                     VLGPLGLKETSLPRGVNLKPPYIHGYDNDPSEQPPEDISEVIASGWPWAAGGMVSTPY
                     DFNDFVRGYVGGDLFSKKVRAQQRQVVPGGSSEPPGPGKNSAGLGIFRYETRCGTVWG
                     HTGNYPGYTQFMAASPNGKRSVTVSVNEQLSPVEGAPGVFDALRRAEVRAVCAALATR
                     "
     gene            complement(13780..14718)
                     /locus_tag="AC16CM_02175"
     CDS             complement(13780..14718)
                     /locus_tag="AC16CM_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3138661.1"
                     /translation="MHVQLEGQIKHLILTGNFEVGSRLPSIRAMAGFLRVNRNTVARV
                     FSALEREGYVESRRGSGVYVLEPPVEADEEKCREVVERVVDLAAARDVPVEDLAYALL
                     ARAGTTPRQKVRILFVECTPAELDQFSDELEEQLPVEVERVLIGDLEERLSGLEEAPW
                     RLAVTTFFHVHEVQELVGPFGLETVALLAEATLESLQRLMDLPPQTPVGVVGWGSTCM
                     DNLSRSIEGAGLDHLDLVQVNVDAGRGEALSTIGEVEAVVCSTKTAGKLREIGVPESL
                     EIIEEDRILDQGGIEMLGRMLRQMPPDTSVGDQPGE"
     gene            14844..15398
                     /locus_tag="AC16CM_02180"
     CDS             14844..15398
                     /locus_tag="AC16CM_02180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138662.1"
                     /translation="MGDPFGLEIAKIRQAELLREAEGRRIARALKRFRGGDVARLGSV
                     GTEGIGVRWGLAGDELAVAELLELNGMPRWISFEERFVVAEEDGRVLGAVRYRTESKR
                     LILGLLVVDPWAGEERLARALYRGAGELAREMGVREVLASASPARYPGMVGYRRRGRS
                     WRLDVTRLPSVGGGSSSIHGVSRG"
     gene            15405..16121
                     /locus_tag="AC16CM_02185"
     CDS             15405..16121
                     /locus_tag="AC16CM_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005554103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR3138663.1"
                     /translation="MQQKVATPTEERVTFTLGGALAGAKKCVPVAITGCAIGLVFGTL
                     AGQAGLGAAEAVLMSAIVFSGAAQFLVLGLWASPLPLAAIVLTTLIVGLRHLLMGAAL
                     GPVFSRLPRLKAYASVYFMADENWALTMGEFDKGRRDGAFLLGGGLLMALAWTSSTFV
                     GVTFGGAVEDPARWGLDFAFTAIFLALIAGMWKGRSSILPWMVAAAVSVAAHHWLPGQ
                     WYILLGGLAGSLAGVVRRGS"
     gene            16111..16425
                     /locus_tag="AC16CM_02190"
     CDS             16111..16425
                     /locus_tag="AC16CM_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HIR3138664.1"
                     /translation="MDLDPTILLAILLMALVTYATRAGGLWIANRFELSERAGAWLDA
                     IPGAILVSLVAPAVLTSGPAEALAAVAVVLVALRSGSLPLAMVTGVVAVVLLRGVMAG
                     LM"
     gene            complement(16309..17058)
                     /locus_tag="AC16CM_02195"
     CDS             complement(16309..17058)
                     /locus_tag="AC16CM_02195"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3138665.1"
                     /translation="MGALLADLVRWVFDVVHAFGYVGVFVLILAGSLFLPVPTELTLP
                     LFGFLVGQDRLSFSPVLLTASAARVSASLIMYEIGLRISEARLQRLVKRLERFRLIFV
                     SDLQGASRVFEKHGGKAILVGHLIPGVGALVSLPAGMKHMPLRWRFLGYTVLGCTLWT
                     GSLIGLGWALGARWRIVEAFTSFVGSAFLILLVCAVLLLFWRRRRTHGRMGLHQAGHH
                     TPQQNDGHDPRDHGKGKATTAQGDEHNSDGR"
     gene            17476..18327
                     /locus_tag="AC16CM_02200"
     CDS             17476..18327
                     /locus_tag="AC16CM_02200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3138666.1"
                     /translation="MVRRLVLVLVLFLAVLGLGLLLFGGPSGPEYVRAVSPVEVRPMP
                     ELPGPSSQEMQNVWDAAVEEDSSPDLATNGKTGTSGDGVALTFDDGPNPRTTPRILDI
                     LREHDTRATFFVVGSQVEEHPDLLRQIVEDGNAIGNHTYDHANLSKLSTTQMRNELER
                     TQKAVDKALGYHYEMKMMRPPYGEPYGNGSDALPAFRKLVRQEQLFPVIWTIDTQDYL
                     MADNPRGIVKNVASLDKKGRDQVILMHDIHPQDVRALPGVIDHLERSGRQFVGVDELL
                     RDKYPVP"
     gene            18428..>18823
                     /locus_tag="AC16CM_02205"
     CDS             18428..>18823
                     /locus_tag="AC16CM_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138667.1"
                     /translation="MEERDARRSPSEPATAPGGKDQAGYTKRLRRLELASSPLDPDAE
                     RRAFLRDRVVAHADGFTEGLDRRPVYVASDDVGSALYDSPISEEPMDAESVLGLLDQV
                     LNRSGITVGSPGHMGYIPGSNLHAAALGDY"
BASE COUNT         3439 a         5842 c         6365 g         3177 t
ORIGIN      
        1 gaaactacga gctgaacatc gaggtgtata gccggagcct tgccgcccag atggagcgca
       61 tgttcgagct tgacaagacc aacgctaaag agctgacctt gcaggaatgg gataatcgtc
      121 ccctcccggc gaaactggtc gagcgtaccc tcgcgagcct gagcccgcta gtttagatat
      181 gtcaggttac aagtatcatc gggcaaaaaa tgctcggaag agggtcggca ggtagactcg
      241 agggtcgtaa agtggacgaa cgaagtcgac tcagtggaga aggattgctc cgcgaacttc
      301 ggctttacgg cgttctcgga agtgccgccc acggaacttg gtgcatgctg accaggaggt
      361 tgatcaacat gtgtcaaggc agtctcttac ctcgaagata gactcgatct ttagtgaggt
      421 gcttctgaga aggagcgccg ctaaagaagg aggtagggca tgcatagagc aggtatgttc
      481 ctcacgctga tgggcgtgtt ggtcttggtg ttcgccgggg tggccacggc agccatcatc
      541 aacggcaacg accgacccaa caccctcatc ggcaccccca ggcacgacgt catccacggc
      601 tacggcggcg gcgacctcat ccgcggtcgc gggggcgagg acgacatgtt cggcggcagg
      661 ggaggagact acatccgcgc ggtggacagg cgcgaggacg acgtctcctg cggcagaggg
      721 aacgacaagg tcagggccaa ccccggagac gaggtcaaca ggggaaactg cgagcgtatc
      781 atcagggcag gcataagggt cgatgataac gacggcgggg acgactagcc tccatcccgg
      841 agccgacagg gacgacgaga agaaggggcg ggtctctcta cccgcccctt ctttatgccc
      901 ttattcaccc taatgcgtgg aggataaatt ctctagagtt cgcatccagg atcccgcata
      961 gcaccagtct tgctgagcgg ctgagaccat ttcagcagcg ggtgaagggt tgtagacatc
     1021 actagatatc gtgatggtgc atagaaatac agggaaatga ccaatgcttc tccgagccgg
     1081 tcttgaagca gcctgcggaa agagcgaacc tggtggaggt aaaagtatca ggtctattag
     1141 ctgaagcctg tggggcctag tcgcgatggt ggccaccgtc ggaatacgcc cagagtcggc
     1201 agccacaacg cccagaagag ccgtacgtgg ctcataggag aacggacatc cctgaacctg
     1261 aagccttaac ttgagctaac acttagtttg tataatgacg acactcttgt tgtagggttt
     1321 ccgctcttgg gaggcttatt ggcgcggtgg cggctcctcg agccggattc gagtgtggtc
     1381 tcggaactct cgcgggcggc ggggctagac ccgctctgtg cccgcgtgct cgccaaccgc
     1441 ggcgtaacgc ctgagggggc cgaagacttc ctgtcgccct cgctctctgc gataagcacg
     1501 ccggacggtg acgcgtgggg cgtcgcggca cggcgggtgg ctagggccat cagggacggg
     1561 gagaggatcg gggtcttcgg cgactacgac accgacggca tcacgtcggc ggccgttttg
     1621 tttctggccc tctcccacta cacgcagaat attacagttg gcttgccgac ccgtcagacg
     1681 ggttacgggc tcctcgagcc atacgttcgg aacctcttcg cagagggcgt ggatcttctc
     1741 atcaccgccg actgcggcat ctccaacagg gccgaggtgg cgctggcggg cgagcttggg
     1801 atggaggtca tcgttacaga ccatcacatc cctcccgaga acccgccgga cacggcggtt
     1861 gccgtgctgg atcccaagct ctgggacccg gaagacccgc tggccggggt cggggtcgcc
     1921 tggaagttcg cgtgggcggt ggtgcgcgag ctcggcgacg ccgagggccg caagctgttg
     1981 gggcgtttgc tcgacctcgt ctcggtcggg acggtggtgg acatcgcgcc gctagtgggt
     2041 gacaacaggg cgctcgcgcg catgggactc aagcacataa acagggggct cgccgcgggc
     2101 ggggcgcgcc ccggcatcgc ggcgctcgtc aaggtcgccg gcgtgagggg tgacctcgac
     2161 gagagcgacc tcgggtggaa gctcgggcca cgcctcaact ccatcggcag gatcaagaac
     2221 cccaggccgg ccctcgatct gctcctgacc caggaccgca gggaggcctt caggatcgcc
     2281 tccgagctca accagctcaa ctccgagcgg cagcggcgga ccaggttcgc cgtcgagcgg
     2341 gcgatggagg aggtcgaccc ggaccaggac ttcaaggtcg tcgtctcgga ggagacgggg
     2401 gggatcgctg gcctgatcgc cggccgcgtc gccggcgcca cagggaggcc ggccgcgata
     2461 ctgacacgcc gggccgacga ctcctacggc ggctcggccc gggccgggga gacggaggtc
     2521 gacctgtacg gtgccctcta ctcggtgagg cacctgatgg gggagtgggg gggacaccga
     2581 aaggcggccg gcctctccgt caagcccggg cggttcgacg ccttcgtcgc cggcgtcaac
     2641 gaggccgtaa gagcccaggc cgaggagaac ccggagatct tcgtgccagc gatcgagatc
     2701 gacgcggaga tacccctgtc gaaggtctcc aacgggctgc tcgagtggca cgagaggctc
     2761 gcgccgttcg gcagcggcaa ctaccgcccc gtcttcgtta cggagggtct gcgtatcggt
     2821 ggttcgcgca agatctggga gggcatgaac ctggtcgacc tcgacggcgt gaaggcgaag
     2881 atggcgtgcg atcccggtga gctgcccacc accgcgttcg acgcggtata cacgatctcc
     2941 cgcagccgtt actctgggcg cgcggagctt gagatagtgg actggaggac gggcagttga
     3001 acgccatcgg tgtagacgta ggggggacca agatcgccgc cgcggtggta accccccatg
     3061 ggaggatcct ggacgaggtt cgctatccga cgccacagtc gttggagacc ctgatcgaga
     3121 acatagcgag ggccgtcagg gaggtcgggg accgttacga agccggaggg gtctgcctcg
     3181 ccgtgcccgg cctgatcctc gggagcgaga acaaggtcat attctccccc aacctgggcg
     3241 ccatcgaggg agtacccctg aaggacgagc tcgagcccag gatcgggcta cccctcacga
     3301 tagagaacga cggaaacgcg gcggcgtggg gcgagtttcg cttcggggtg ggcagcgagg
     3361 ccgaccacct ggtcttcgtc acgctgggga ccgggatagg cggcggcgtc ataacccacg
     3421 gtgtgctgat gcgtggcgcg cagggctcgg gcggtgagct cggccacgtc acgatccagg
     3481 aggggggacc ccgctgtgcc tgcggcaact acggctgcct ggaagccctg gcttccggca
     3541 cggccatagc gcgccggggc cgcgagcggg ccatcgaaaa ccccggatcg gccctcggca
     3601 gcctggccac gaggcgtcag atcctgggcg aagacgttac gcgcctggcg cgcgaggggg
     3661 acgcggtcgc gctctcggtg ctcgaagaga ccggaaggtg gctcggcatt gggctcgctt
     3721 ctttcgtcaa catcttcaac cccgaggtcg tcgccgtagg gggcggcgcg atggaggcgg
     3781 gagaactgat cctgaaagct gcccgccggg agataatgga ccgcgcaagg cccccttcac
     3841 gggacctagc cgaggttcag gtcgccacgc tcggtcccaa atccggcgtc ctcggagccg
     3901 ccgccctggc cagggacgcc tcctctgggg agtacttgct gggaacctga agcatgcccc
     3961 tcagcgtgat ccccacccca ataggcaacc tggaggacat aaccctgcgt gccctcaggc
     4021 atctgcgcga ggccgacctc gtagcctgcg aggacaccag acgcaccggc cgtctcctcg
     4081 cccactacga gataaaacag aacctcttcg cctaccacga gcacaacgag gaacgcctcg
     4141 cccccgagct cgccaaacgg gccaggaccg agaacatcgc actagtcacc gacgccggta
     4201 ccccgctcgt ctccgacccc ggctacaggc tcgtccgcgc ctgcatcgag gccggcgtca
     4261 cggttgaggt tctgcctggc ccttcctccc ttaccaccgc gctcgccgtt tcggggttgc
     4321 ccactgactc cttcgtgttc ctcggtttcc ttccacgcaa gggccgtatc aggacggaga
     4381 cgctcgaacg catccagcgc gaggagacca cgttcgtgct cttcgagtcg ccgaacaggc
     4441 tggccaggac gcttcgggac ctgcctcccg gagtccctgt cgcggtgtgc cgggagttga
     4501 ccaagctgca cgaggaggtg ttccgtggga cggctgccga ggccgcggag cacttctctg
     4561 gcaaggtaaa gggtgagatc gtcgtggtgg ttcgtggtga tgacgaacgc gggagcgcga
     4621 ccctcgaaga agccgtcgag attgccaggg ggcacgtctc ggagggcgag agcccgtcgc
     4681 gggccgcggc gctggcggcg agggagtcag gacacagaaa ggccgagatc tacgcggggc
     4741 tggtgaactc cagaagcgaa tgaaggtcag aaacccgcca tctttcggta tgaagccagg
     4801 ctggcatcca acggtggtaa agggacctcg agagggaaag cggctctttc ggccaccctt
     4861 tccctgctcc ctgtataatg cccttcgctg acgactcagg ggaagaggag ataggcacta
     4921 tgcttgtgga ctcgcatacg caccttctgc ggctcgaggt ctccccggag gaggcggtga
     4981 agagcgcggc cgacgtgggc gtcggggcgg tggtcaacat cgggaccgag gtcgaggact
     5041 cccagagagg ggcagagctc gcggcggtct tgcccaacgt ctactcgacg gcaggtattc
     5101 atccccacaa cgcgggcacc tatacggcgg ccgacctcga ggcgctcgcc gagctctcgg
     5161 agtcgcccgg tatagtggcg ctcggtgagg tcgggctcga ctactaccgt tcggagtggt
     5221 ctgaggaggt gcagcgggca cttttcgagg acgttatctc ccttgccaac gacacgaggc
     5281 tgccgctggt gatccacacc agggaagcct tcgccgatac gatggcgtgc ctcggtagcg
     5341 cacgcgttcc gatcgtcttg cactgcttcg agggtggtga ccgggaggtc agggaggcga
     5401 ccgagcgcgg ctactacatc ggtcttgcgg gaaacgtcac ctacaagggc agcgagacgg
     5461 ccagggtgct ccgccttctc gacgaggagc gtatcctggt cgagaccgac gctccctacc
     5521 tgagtcccca gcccgcgcgc ggcaggaaga acgtgcccga gaacgtcgtg tacactgccg
     5581 gcttcgtcgc cgagaggctt ggtatggatg acgatgactt cgccaccctc accacccgca
     5641 acgcccgcaa cctcttcggc ctaccgctgg aggtgtaggt ttcgttgtcc gaacaggggc
     5701 cgccgaaaaa acgcttcgga cagcattttc taaaggaccc taacactgcc aggatcgtcg
     5761 cctcgggcgt aacgaaagac gacgtggtgc tggagatagg tccgggacgt gggttcctga
     5821 cgagcttcct ggccgagagg gctggcctgg tgcacgccat cgagctcgac ccggatgttc
     5881 tcccggccct tcgcgaggca gcacgtggca ggaacaacgt gatcgtctac aagggagacg
     5941 ccctccgctt cgactactcc gccctcgacc cggagccgac caggctggtc gccaaccttc
     6001 cctacaacat cgcctcgccg ctcgtgctgg ccctgctcga ggaggtcgac agcatacgta
     6061 cgttgcggtt tatggtgcag ttggaggtgg cacgcaggat ggcggccgat cgggggagca
     6121 aggactacgc ggcctacgcg gtgctcgcgc aactgctggc gagcgtgagg gtcttgcacc
     6181 gggttccccc cacggtgttc gacccgcccc ccagggtaca ctcggcggtc gtggagatgg
     6241 atcgcaagag caaaccggac ggttaccagg ggatcaaacg cctcgtcctc ggcgctttca
     6301 ggagccgtcg caagcggctg gtcaacaacc tgcccgaacc ggtgcgcagg gaggccccgc
     6361 gcgtgctgca atcgcttggc tacgggccgg acgcgagggc cgaggagctg gcgcctgagg
     6421 atttcgtggc gttgtaccgg gcgctttcgt gggcgttgtg atgcgggagg ttcggctcag
     6481 ggctttcgcc aaggtcaact tcgcgctcga cgttctggga ctgcgcgtgg acggctacca
     6541 cgacatcgca accgttatgc agagcatctc cctctctgac gaggtggaca tagaacggat
     6601 cgccgagggg ttcgagttcg acttcgagcc agagggtgag gagatgggac cgcccgaacg
     6661 aaacacggca tacgtggcct ggaagtcgct ccagaggctg acgggcctgg cgcttcccgt
     6721 gcgcgtcagc ttgcggaagt ggatcccggt gggggccggg cttggcggcg gctccgcgga
     6781 tgctgccgcg gtccttgtgg ggttgaacga gcttttcgat ctcgggctgg ggatggaagg
     6841 gttacgtgag gtgggggcca gggttggggc cgacgtgcct ttctgcatct cggggggaac
     6901 cgccctcggt gagggggtag gggagaggtt gacgcagctt cccagcccac cagcccagag
     6961 actcgtcctc gccaagccgc accgtagcgc ggataccgga gagatctacc gcgcccacga
     7021 tgcgatggga gcaaagagcg ccctttgcgt cgaacccgtc gtctcggcgc tccggtccag
     7081 aagccttcgg gacctcgccg cggccgtggg caacgacctc gtctcggtga cgcgcgagct
     7141 tgtaccagag gtaaagacgt tgcaacgtga ccttctgagg tgcggagcgc tcggggccac
     7201 gatgagcggg agcggtaccg ccgtgtacgg catcttcgac gatgaaggtg ccgccgaaag
     7261 agccgcaaag gccgtcgatg taccgttcgc gggggtctac cagccggttc cgcacggggt
     7321 agaattcgtt tgaagaaggc aacaggcatc aggctacagg aaggctcttc agcgcttcgc
     7381 ttctcgaacg gtgagatggg gtgacctccc ggtcttctgg tgcgtgttcc acaaggaacg
     7441 gtccggggat atctctctcc gatggggttg ccgggaaagt ttgcgtagag ataatcctac
     7501 ccccggcacc cgagccgaac ccgaaacccg tgtaggggcg gttcgcgaac tgcccaacgt
     7561 gcgcatagcc cctggagcgg tctcgcaaga aacgtggaac cgggggcgca tcgaggcaac
     7621 ggacgacggg tcttctaatt cctgtagcct gatgcctcta cacctgacgg cgaaaaggtg
     7681 ctccggtata atctgctggg gtcgtactgg ggcgtggcca agaggcaagg caccgggttt
     7741 tggtcccggg atcgcaggtt cgaatcctgc cgccccagcc gccagagata acacgagcga
     7801 tagggatggt gcgggtacat gtctcagagt tcaccgatct tcgccgtggt cctggcggct
     7861 ggcaagggca ccaggatgaa gtccaaccgg gcgaaggtcc ttcacacgtt gtgcggcatc
     7921 cccatggtga actacgtgat cggggcgacc aggccactct tgccggaggg gacgctcgtg
     7981 gtggttggcc accaggccga cctcgtcgag gacgtgttgc ccgacgacgc gacggccgtc
     8041 ttgcaggagg aacagctcgg gaccggtgac gccgtgcgcg tggccctgtc cgccatcccg
     8101 gaggaccagg agggcaccct tctggtcgtc aacggcgacg gtcccctaat ctcggacagg
     8161 actcttggag agctggtcga gcggcaccgc tcggcgggcg ttggcgctac ggtgctcgtg
     8221 gccgagcccg acgacccgag cggcctcggg agggtggtcg aggatgctgg cgtggtcagg
     8281 atagtagagg agaaagacgc gaccgaggcg gagcggagga tagggctcgt caacctcgga
     8341 ctctacgcct tcaggatacc ggagataagg gaagcggtcg ggggactctc cacggagaac
     8401 atgagcggcg agctgtacct gacggacgtg ctcgagacca tcgggcggcg ctccagggcc
     8461 gtaacctaca ggctcaagga catggaggag tcgcatctcg tcaacgaccg cgcgcagctg
     8521 gcggtggccg aggagatcct caggcgccgc atcctcgacg cgcacatgag ggaaggggtt
     8581 acggtaagag acccagcctc gacccacata gacgccaccg tcgaaatcgg gcgcgacacc
     8641 gtgatcctgc ccggtaccct cctgcgcggg ggaacgaagg tgggcacgga ctgcgtgatc
     8701 gggccgaaca ccgacctgca ggataccctg gtcagtgaag gagccctcgt ggagcactcg
     8761 gtggggcgcg gggccgaggt cggcgccggg gcgaccgtcg gaccctactc gttcttgagg
     8821 ccggggacga agctcggtgt cgggtccaag gtcggcgcct actgcgagct caaggactcg
     8881 cgggttggag aggggagcaa ggtgccgcac ctctcctaca tcggggacgc ggagatcggg
     8941 gccggcgtca acgtcggggc cgggaccata acagccaact acgacggggt gaacaagaat
     9001 cggaccacgt tgggcgacgg cgtctttacc ggggtcaaca ccgccctcat cgcgcccgtc
     9061 gaggttggcg aggacgccta cacgggggcc ggggccgtga tcaacaagaa cgtaccggcg
     9121 ggcaagctcg cggtggggtc accggcgcgg gccatcgcgg agtcgcccca gaagcgcagg
     9181 aagaggcaag acgggccgca agagaaagag acggggggct agcgctttgg ttatgcagaa
     9241 cggctatctg acgcagacca cggagaagcg gctgatgatc gtcagcggcg atggttaccc
     9301 cacgctggcc gagagcatag ccgaccggct cgacatggag ctcacagggg tcgagctgac
     9361 ggagttcgcc aacggtgagc tctacgcccg ttacctggag agcgtgcgcg gggccgacat
     9421 gttcatcgtc cagtccctgt gtgagcccgt caacgacaac ctcatgcagc tcctgatcat
     9481 gatagacgcc gccaagaggg cctcggcgaa gaggatcgtc gccgtgatcc cttggtacgc
     9541 ctactcgcgc caggacagga agaccaagcc gcgcgagcct attacggcga ggctggtggc
     9601 caacatgata gcggccgccg gcgccgagcg ggtgatgacg atggacctgc acacgggcca
     9661 gatagagggc tacttctcct tccccgtgga tcacctgacg gcgatgcaca cgttcgtgga
     9721 ctatttcgtg ggccagggct tcaggggcgc cgaggatggc gtggtggtgg ccccagatac
     9781 tggcgaggtc aaggtcgcca agcagctcgc cggccacctg ggtctcccgt gggccatcgt
     9841 gaacaagatg cgtgacaggc cgggccattc cgaggtgacg cacgttatcg gcgatgtggc
     9901 tggcaagcgc gtgatcatga tagacgacgt tatcgccacg ggcggaacca tcgcgaacgc
     9961 cgcgcagcgc ctggtggacg agggtgccac cgaagttagc gccgccgcga cccacggcga
    10021 gttctccggc gacgcctacc gcaagctcga tgaatcaccc ctcaaggaga tcgtggtgac
    10081 gaatacgctg cccttgaatg agaaccagcc gcaggctaag atcaaaacgc taaatatcgc
    10141 accaattctc gctagcacca tcaagaacgt gtttacggac gagtcggtga gcgaggtatt
    10201 tatgggcgag aaccaactct tctagaccgt taggagcgag aaatggcaga taacatagac
    10261 ctgcaagcac aggaacgcta cgagcgtggc aagaacgcgg cccgccgtct gagggcctcg
    10321 gggatgaccc ccgccgtgct ctacggcgag ggcaacgagg caggcgggag cacggcgctt
    10381 gcgatcccga ccagaatagt ggactacacg cttcaccact acggggataa cgccctctac
    10441 aacatagcca tcgacgggaa cacctcgacg gcccgggtcg ttgacgcgca tcgcgacccc
    10501 gtctccgggc ttctggtcca cgtggacttt gccccggtga atatgaccga gcgcatcgag
    10561 gtcacggtgc cgctgtcggt cacgggcgat gcgcccggcg tggaagaggg cggtgttctg
    10621 cagcaggtgg cctacgagat ccaggtcgag tccctccccg gggatatccc ccaggagatc
    10681 acgctcgacg tctccgaact gggcatgaac gagaacctta ccctgggcga cctgacgctc
    10741 cctgatggcg tggcactgct ctcggagccg gaagaggtcg ccgtgacggt gacggcgccg
    10801 acggagatct ccgaggagga gatggaggcc gccggcatcg tcgaggagcc gacggacgag
    10861 gaagaggccg cgggaggcga ggaggctgcc gagggtgaag agacccccga ggaatctgcc
    10921 gaggagggcg aaggcccggc cggacagaac gttcaggcct aggctggtcc gctctttctt
    10981 cagcggggag gcgaaaggcg acggggggct cctcgagtcc cccgtcgtcg tcggcctggg
    11041 caacccggga cgctcctacg agcgcacccg ccacaacgtc ggctacctgg ttgccgacga
    11101 gctcgccacg cgccacgccg gctcctggcg caaacgtaag aaggccgagg ccgcacccat
    11161 cacccttggt ctggtcgagg ccacgcttct caagccgacg acgttcatga acaattccgg
    11221 ttccgccgtc tcgggctaca ggcccgagga cctcgtggtg gtccatgacg acctggatct
    11281 ggagaccggc acggtgaggg tcaaggtcgg gggtggggcc ggtgggcaca acggcctgcg
    11341 ttccatcatc gggaggctgg gcaacgactt cgtcagggtg cgcgtcggta tcggacgccc
    11401 gcccgttggt gttggcgtca ccgactacgt gctctcgcgc atggattctg gtgtgaaaga
    11461 agccatcccg gccgcggccg acgcggtcga gttcctgctc gagcaaggtc cagaagcggc
    11521 catgaaccgc ttcaacgttc gcggatagca acccggctca tctaccaggg gagagattgc
    11581 gtgggatcat tggagcgtcc tgatcaaggc cgagccgtag gacgggtgcg cgatgtggta
    11641 tcgcgacttc tgcatggtgg caccgcagtc gcccgctcag atggctctct ccacgacctc
    11701 ttcccgatcg tggttagcgc cgccgaggga gaggcgctga gagactgggt gttgcgggag
    11761 cgggcgacca gtacggtcga ggtcgggctc ggctacggta tctcggcgct ccacatctgc
    11821 gagggcctcc tgcagaactc gggcccgtct cctcaccatg tggcaataga cccataccag
    11881 gccacgcggt tctccgactg tggactgcag ttcctcgacg aggcgggggt aagggagatg
    11941 gttgagcttc acaccgaaga ctcccagatc tcgcttcccc gcttcctcga tggagacagg
    12001 accttcgacc tcgcctttat cgacgggaac caccgcttcg agcgggtctt cgtcgacctc
    12061 ttctacctcg gacgactcgt acgacccggc gggataatct tcctcgacga ctaccagttg
    12121 cccggcatcg cccgtgcagc ctcgttcttc gtctcgaacc tgggctggac cactgaggag
    12181 atctcacccc cggacgacct gcaccagtgg gcggcgcttc gtacttccgc ggtcccggac
    12241 gagcgatcgt tcgactactt cgtcgacttc tagagcagct tgcgaggacc cgagcttgtg
    12301 gaaggcgagc tatctgcaat aggcgagaaa cttccagaaa ccgatagctg aaagcccgct
    12361 tcgaagatga ccgatcctcc tgcaagcttc tgtagagcgc ggccgtagcc cttcgcacag
    12421 cgccggagtc cgcccgtgac tataattctt cggccgtaca acgggggctg ccggatcctt
    12481 gtcctcgccc cgctacagaa aggaaccagg atgaggaaga catcgctgct gctcgcctcg
    12541 atcgccttcg cggtgatgct tgccggggga gctgcgctgg ccgcgagttc cgaagcgggg
    12601 aaccccggta acgcggaggc ccgcgatgcg gtgctcgacg cctccatgaa ggcgttggtt
    12661 gagaggcgtg gtggtcctcc cggggcgatt gctgtcgtcc agcgcggcaa cgacagggag
    12721 gtccacgctt tcggggtcag caacttgaag cgagatcttc cgatgcgggc ggacgcccgt
    12781 atgcgccagg cgagcacctc caaggctttc aatggcgccg tcgccctctc gctggtggac
    12841 aagggtgaga tgtcgttgaa cgacacgata ggcgaatacc tgcctggctt gccgaagtcc
    12901 tggcacgagg ttacgctgcg ccagcttctg gaccacacga gcgggctccc gaacttcacc
    12961 gaggatccag gctacctgcg tgcgctctcg gccagccccg aaaacgcgcc gacgccccgg
    13021 aagctgctct cctacgtgga ggacgagccg ctgaacttcg agccgggctc cgactatcgc
    13081 tactccaact cggacaacgt agcggtgggc ctcatggtcc gggtcgcgac cggagacacc
    13141 tacgaagagc aactccagga acaggtactc ggaccgcttg gcctgaagga gaccagcctg
    13201 ccccgaggcg tcaacctgaa gccgccctac atccacggct acgataacga tccctcagag
    13261 cagccgcccg aagatataag cgaggtcatc gcctcggggt ggccgtgggc cgccggcggc
    13321 atggtctcca cgccctacga tttcaacgac ttcgtgcgcg gctacgtcgg gggtgacctc
    13381 ttcagcaaga aggtacgggc gcagcaacgc caggtcgtgc cgggcggaag ctcggagccg
    13441 cccggacctg gcaagaactc ggcgggcctc ggaatcttcc gctacgagac gaggtgcggg
    13501 accgtctggg gccacacggg taactacccg ggctatacgc agttcatggc cgcgagcccg
    13561 aacggcaagc gctccgttac ggtatccgtg aacgagcagc tcagcccggt cgagggggcg
    13621 ccaggggtct tcgacgccct caggcgtgcg gaagtgcgcg ccgtctgcgc cgccctggcc
    13681 acccggtaag ggcgaagcgg gaatagtcgg tccaattttc tcgtggtagg ggtgggaggt
    13741 gcccgccttc agcctacacg gggagtagag gtctctcctc tactccccgg gctggtctcc
    13801 gacagaggtg tcagggggca tctggcggag catgcggccc agcatctcga taccgccctg
    13861 gtccagtatc ctgtcctctt caatgatctc aaggctctcg ggaacaccga tctcacggag
    13921 cttaccggct gtcttggtgg agcagacgac ggcttcgacc tccccgatgg tggacagagc
    13981 ctcgccccgc cccgcgtcca cgttgacctg aaccaggtcg aggtggtcga ggccagctcc
    14041 ctcgatggag cgggagaggt tgtccatgca cgtgctgccc catccaacta ccccgaccgg
    14101 ggtctgggga ggcaggtcca tgaggcgctg caggctctcg agcgtcgcct cggccaacag
    14161 cgcgaccgtc tccaacccga acggtccaac gagttcctgt acctcgtgaa cgtggaagaa
    14221 cgtcgttacg gcgagccgcc acggcgcctc ttcgaggccg gagagcctct cttcgaggtc
    14281 ccctatcagg accctctcca cctcgaccgg caactgctcc tctagctcat cggagaactg
    14341 gtcgagctcc gcaggagtac actcgacgaa gaggatgcga accttctgcc gcggcgtcgt
    14401 accggcccgc gccagcagcg cgtacgccag atcctcgaca ggtacgtccc gcgccgccgc
    14461 aaggtccaca acccgctcca ctacctcccg gcacttctcc tcatccgcct ccacgggggg
    14521 ctcgagcacg tacaccccgc taccgcgccg gctctccacg tacccctccc gctccagggc
    14581 cgagaagacg cgcgccaccg tattgcggtt cacccgcaga aatccggcca tcgccctgat
    14641 gctcggcaac cggcttccca cctcgaagtt gcccgtcagg atgaggtgct tgatctggcc
    14701 ttccagctgt acgtgcaccg gtacgtgact ctttgaattt attctcaaat ccataatcag
    14761 cctattgtcc tatgacacca tgccagttat aattgtccca agtcagcgtg acaattatac
    14821 cccagttggt gaggaggctg gctatgggcg atccgttcgg gctagagata gcgaagatca
    14881 ggcaggcgga gttgttgcgg gaggctgagg ggcgcaggat cgcgcgggct ctgaagaggt
    14941 ttcgtggtgg cgacgttgcc cgtctggggt cggttgggac agagggtatc ggggtacgtt
    15001 gggggcttgc gggggacgag cttgcggtcg ccgagttgct cgagctaaac ggcatgccgc
    15061 gttggatctc tttcgaggag cggttcgtgg tcgcggagga ggacgggcga gtccttggcg
    15121 ccgtgcgtta caggacagag tcgaagcgtt tgatcctggg cttgctcgtt gtcgatccgt
    15181 gggcgggcga ggagcggctg gcacgcgcgt tgtatcgtgg ggctggcgag ttggcccgcg
    15241 agatgggggt cagagaggtg ttggcatctg cctcgccggc acgctatccg gggatggtgg
    15301 gttatcgccg acgcgggcgt agctggcgcc tcgatgtaac acggttgccc tcggtcggtg
    15361 gcggaagctc gtcgattcac ggcgtgtccc ggggttagag aggcgtgcag cagaaagtag
    15421 ccacgcctac ggaggagcgg gtcaccttca cccttggggg tgcgctcgcg ggggcgaaga
    15481 agtgcgtgcc cgtggcgatc accggctgcg ccataggcct cgtcttcggg acgctggccg
    15541 ggcaggccgg gctcggggct gctgaggccg tcctgatgag cgccatcgta ttctccgggg
    15601 cggcccagtt cctcgtgctg gggctctggg cgtcgccctt gcctttggcc gcgatcgtgc
    15661 tcaccaccct catcgtcggc ctcaggcacc tcttgatggg tgccgcgctc ggtcccgtgt
    15721 tctccaggtt gccgcgcctc aaggcctacg cctccgttta cttcatggcc gacgagaact
    15781 gggcgctcac catgggcgag ttcgacaagg ggcgccgcga tggggctttt ctgctcggtg
    15841 gggggctgtt gatggccctc gcgtggacca gctctacctt cgtcggggtc acgttcggtg
    15901 gcgcggtcga ggatcccgcg cgctggggcc tcgacttcgc gttcacggcc atattcctcg
    15961 cgctgatcgc cggtatgtgg aaggggaggt ccagcatcct gccctggatg gtggcggccg
    16021 cggtatccgt ggcggcccac cactggctgc cggggcagtg gtacatcctg ctcggtggcc
    16081 tcgcgggtag cctggctggg gtggtgcggc gtggatcttg atcccaccat cctgctggcc
    16141 atcttgctta tggcgctggt gacctacgcc acccgcgcgg gcgggctctg gatcgccaac
    16201 cggttcgagc tctcggagcg ggccggggct tggctagacg cgatacccgg cgcgatactg
    16261 gtctcgctcg tcgccccggc ggtcctgact agcgggcctg ccgaggcgct agcggccgtc
    16321 gctgttgtgc tcgtcgccct gcgcagtggt agccttcccc ttgccatggt cacgggggtc
    16381 gtggccgtcg ttctgctgag gggtgtgatg gccggcctga tgtagtccca tcctcccgtg
    16441 cgtcctccgc cggcgccaga acaacaagag gaccgcgcag accagcagga tcaggaaggc
    16501 cgacccgacg aaggacgtga acgcctccac tattcgccac cgggcaccca acgcccatcc
    16561 cagcccgatc agggagccgg tccacagcgt gcacccgagc acggtgtagc caaggaaacg
    16621 ccagcgcagg ggcatgtgct tcatgcccgc gggcaacgag accagggcgc cgacgccggg
    16681 gatcaggtgt cccaccagga tggccttccc cccgtgtttc tcgaagaccc tgctcgctcc
    16741 ctggaggtcg gatacgaaga tgagcctgaa gcgttcgagc cgcttgacga ggcgctgcag
    16801 gcgcgcctcg ctgatcctga gcccgatctc gtacatgatg agcgaggcag ataccctggc
    16861 agccgatgcc gtgagcaaga ccggcgagaa cgagaggcga tcctgcccga cgagaaaccc
    16921 gaagaggggg agcgtcaact ccgttggcac cggcaggaac aggcttccgg ccaggatcag
    16981 gacgaacacg ccgacgtagc cgaaggcgtg gaccacgtcg aagacccatc tgacgagatc
    17041 ggcgagcaga gcgcccatgg cgccttatcc tcctatctct tccacgatac aacctaatta
    17101 tgcacgatac cgcgcaccgc acgaccccga tcccacggtt ccctgttcgt gacgcttgcg
    17161 tggcgtagct gcggagatta tgcgatatgt aggctctatt cggggtaggg caccacatgc
    17221 ggtggtgact cgcacgttct ttgcgtaacg attaacatgt acgctatggg gagcacggcg
    17281 tatgatgcgc ggggccggcg ggtgggtacg aaatatcggt cgcggaggcc acaacccacg
    17341 tcgcgcccgc gacgggccag gtcacggcgg ccgagaccgc agcagaccgt ttccgggcag
    17401 aggatagcga accgcccggt ccggcggcgg acaccgcccc gctacgccct caggcacgcg
    17461 cgccggcgcc gtttcgtggt gcgacggctc gtactcgtgc tggtgctctt cctcgccgtt
    17521 ctggggctgg gcctgttgct tttcggtggc ccttcgggtc ccgagtacgt gcgggctgtc
    17581 tcaccggtgg aggtcaggcc tatgcccgag cttcccggtc cttcgtccca ggagatgcag
    17641 aacgtctggg acgcggcggt ggaagaagat tcctcgccgg atctggcgac caacggcaag
    17701 accgggactt cgggggacgg ggtcgcgctc acctttgacg acggacccaa cccaagaacc
    17761 acgccgcgca tcctcgacat cctgcgagag cacgacacga gggccacttt cttcgtggtc
    17821 ggtagccagg tcgaggagca ccccgacctc ctgcgccaga tcgtcgagga cggcaatgcc
    17881 atcggcaacc atacctacga ccacgccaac ctgtccaaac tttccaccac gcagatgcgc
    17941 aacgagctcg agcgtaccca gaaggccgtc gacaaagccc ttgggtacca ctacgagatg
    18001 aagatgatgc gcccacctta cggggagccc tacggcaacg gttcggacgc gctacccgcc
    18061 ttccggaagc tcgtccgcca ggagcagctc ttccccgtta tctggacgat cgacacgcag
    18121 gattacctca tggccgacaa cccgcggggt atagtgaaga acgtcgccag cctggacaag
    18181 aaaggacgcg accaggtgat cctcatgcac gacatccatc cccaggacgt tcgcgcgcta
    18241 cccggagtta tcgaccacct cgagcgctct ggccgccagt tcgtcggcgt ggacgagctg
    18301 ttgcgcgaca aataccccgt accataggac ttcccggata acggtcccgt ccgcgcggca
    18361 aggtcgcgac ggcttctgga aacttcgtat atcctcctgg aggtaagcga gacgaggaag
    18421 gtggcctatg gaggaacggg acgcacgccg ctccccttcg gagccggcca ccgcacccgg
    18481 cggtaaggac caggcagggt acacgaagcg gttgaggcga ctcgagctgg cgtccagtcc
    18541 gttggacccc gacgccgaac ggagagcatt tctgagggac agggtcgttg cccacgccga
    18601 cggcttcacg gagggcctgg atcgcaggcc cgtgtacgtg gcctccgatg acgtcggtag
    18661 cgcgctgtac gactcgccca tctccgagga gccaatggat gccgagtcgg tcctggggct
    18721 gctggaccag gtcctgaaca ggagcggcat aaccgttggc tcgccgggcc acatgggcta
    18781 catcccgggg agcaacctgc acgccgcggc cctcggtgac tac
//
LOCUS       DBNGVD010000075         6096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4570425, whole genome shotgun sequence.
ACCESSION   DBNGVD010000075 DBNGVD010000000
VERSION     DBNGVD010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4570425"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="AC16CM_02210"
     CDS             complement(<1..905)
                     /locus_tag="AC16CM_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3138668.1"
                     /translation="MNRNPKRSEPAGALRRWARFAFRHRWAVLVAWAVAIATLAAFSV
                     AFGGRFVDSFEIPGSESQDAVDLLEDHFPDQAGDSATLAFKANRGIRDPEVRDRVEDA
                     VSRARDLPEVTEVASPFVIPGAISEDGKIAYANVQYGKQGPEVGEKSVEKLLDLADDA
                     DGGGLRVEAGGQVVSSSEVSPPGESEIIGIVAAVVILLVAFGSVVAMGLPIVTALVGL
                     GSGFLLISLAASVMDMTTFTPAFASMVGLGVGIDYALFVVTRFREGLSAGFGVEKAVV
                     GAVDTAGRAVVSAGIVVVIALLGLLT"
     gene            1102..2121
                     /locus_tag="AC16CM_02215"
     CDS             1102..2121
                     /locus_tag="AC16CM_02215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3138669.1"
                     /translation="MGFGLSVLDVSPVSSGSDGARALRNTLEVARLADELGYERYWLA
                     EHHNLPTIASSSPEIMIGHVANVTERIRVGAGGIMLPNHAPLQVAEAFRVLEALHPGR
                     IDLGIGRAPGTDPVTAMALRRSDEGPGANDFPERFGELLAFSGDGFPEDHPFRSVVAM
                     PNDIELPPIWLLGSSGYSATAAGKMGLGYAFAAHFSPSDPAPAMHAYRENFEPSKGFA
                     RPSAVLAVAVVCGETEDHARRLASSMELAWVRMRSGRPGPLPSPEEAMGTSYTPGERR
                     LADAYRSMQIAGDPASVRAQIEGLAERTQADEVMVTTNVYDHAERLGSYERLAAAFEM
                     AAPAR"
     gene            2158..3108
                     /locus_tag="AC16CM_02220"
     CDS             2158..3108
                     /locus_tag="AC16CM_02220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3138670.1"
                     /translation="MADENPQQGGLKGSSGTREVYPDGSILGGEIGLWSSDLNALPAA
                     GAQEAAAEIEELGYAALWFGETLGREAFTNASMLLSATSRLVVATGIANIFVRDAWAT
                     NAAARTIAGAHPGRFVLGLGVSHKPMVQMRGHDYRSPLSTMRAYLEDMSEAQFTAAQP
                     DQEPPCLLAALGPRMLELSRDLADGAHPYLVTPQHTAEAREILGEGPLLAVEQAVAPT
                     EDREEALRLARSHLSRYLQMPNYRKSWLRQGFREEDLSGEGSEGLATGLVAWGSEDEI
                     LGRVREHLSAGADQVCVQVLTDGVEDLRGRWRSLAPVLLG"
     gene            complement(3565..4848)
                     /locus_tag="AC16CM_02225"
     CDS             complement(3565..4848)
                     /locus_tag="AC16CM_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIR3138671.1"
                     /translation="MEIEHKAEIFERIGHAVRARREESVGLLQRLVRVASPSGGEVAV
                     QEVVEDALLGCGLAVERCEATTAQVAPYAEHVGWESKPEGRPNIVGVRAGSGGGRSLL
                     LNGHVDTVGPGDPAMWTYPPFSGEVVGDLLYGRGACDMKGGIATVISALEALDVLGLD
                     LRGDVTVATTVGEEDTGVGALATVLAGYRADAAVVTEPTRLRLVPAQAGSLVFRITVR
                     GRAVHASARHRGVSAFEKFLPIFEALGELEREREAKLRHPLYDGVPDRAAINLGVVRA
                     GEWSITVPETLFAEGRVGLIPGEELDDSKSMVADRIMEAAEKDPWLREHPPEIEWPGG
                     QSVPVEVPVDAPICQAVSHAHERVTGKRPGVEGSAYGADMRFFVRFAGMPCVMYGAGD
                     IGWAHCPDEHISITDLLTATETIACLLVDWCGVSG"
     gene            5033..>6096
                     /locus_tag="AC16CM_02230"
     CDS             5033..>6096
                     /locus_tag="AC16CM_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3138672.1"
                     /translation="MDVMDGLGKRGDRTKLAGVDNLMLHFTPYAEDWSKLPVIVSGDG
                     SYVTDDKGRTYIDGLAGLYTTQVGHGRTELADAAHKQMKELGFFPNWSFQHPRSLELA
                     AKIAEIAPGDLNSTFFVSSGSEAVETVIKLARQYHKANGEPGRYKIISRDVAYHGTTM
                     GALSVTGLPGFKAPFEPLPSGFFHVRNTQQDPEGAADAIEQMIETEGPELVAAVILEP
                     VQNAGGCLVPPPDYWKRVREICDKHGVLLVSDAVICAFGRLGEWFGIERFGVVPDMTS
                     FAKGVTSGYLPMGGVVVNDRIAHTLKDRAPMFMHGSTFGGHPVSSAVALENIAIMERE
                     NLLQNVHDLEGHFGDELKKMA"
BASE COUNT         1072 a         2070 c         1973 g          981 t
ORIGIN      
        1 gtcaggagcc cgaggagtgc tatgaccact acgatgccgg cggagaccac cgcgcgtccc
       61 gccgtgtcta cggcccccac aacggccttc tcgacaccga agccggcaga gaggccctcc
      121 cgaaagcgcg ttacgacgaa gagcgcgtag tcgattccga cgcccagccc gaccatcgag
      181 gcgaaggcag gggtgaaggt ggtcatatcc atgacgctcg cggcgagcga gatcaggagg
      241 aaacccgagc cgaggccgac cagggccgtg acgatgggta gccccatcgc caccaccgaa
      301 ccgaaggcga cgagaagtat cacaacagcg gcgacgatgc ctattatctc cgactcaccg
      361 gggggcgaga cctcgctcga cgacaccacc tgaccgccgg cctccacgcg tagcccgccg
      421 ccgtcggcat catcggcgag atcgagcagt ttctccacgc tcttctcgcc cacctccggc
      481 ccttgcttgc cgtactggac gttggcgtaa gctatcttcc catcttcgga gatcgccccc
      541 ggtatcacga aaggcgacgc cacctccgtt acctccggca ggtcccgggc tctcgagacg
      601 gcgtcttcca cacggtccct gacctccgga tcccggattc ctctattcgc tttgaacgcg
      661 agggtcgccg agtcgcccgc ctggtcggga aagtggtcct caagcagatc cacggcgtcc
      721 tgcgactcgg aaccaggtat ctcgaaggag tcgacgaagc ggcctccgaa ggcgaccgag
      781 aaggccgcga gggtggcgat cgccaccgcc caggcgacga gcacggccca gcggtggcga
      841 aacgcgaacc gggcccacct gcggagggcc ccggctggtt cacttctctt cgggtttcta
      901 ttcaccgtac gagattaccc ggtgcgtagg ggtcctggcg taaccgaggt cacgtcaggg
      961 tgctgctccc ttcgtccact ggccgcatcg cgcagtacga aagtgggcaa caggagaagg
     1021 tcgaggagcg taggaaccgc agggttagaa tctgcggggt ggcacgactt tacaggcgat
     1081 ctctcgaagg agctctgcag tatgggtttc ggtctctcgg tactggatgt ctcgccggtc
     1141 agctctggct cggacggggc cagggcgctt cgaaacacgc tcgaggtggc ccggctagca
     1201 gacgagctcg gctacgaacg gtactggctg gccgaacatc acaacctgcc caccatcgca
     1261 agctcgtccc cggagatcat gatcgggcac gtggcgaacg tgaccgaaag gatccgtgtc
     1321 ggcgcgggcg ggatcatgct ccccaaccac gccccgctcc aggtggccga agcctttcgc
     1381 gttctggagg ccctgcatcc ggggcgcatc gaccttggca tagggcgggc acctggcacg
     1441 gaccccgtga cggccatggc cctgcgccgc tctgacgagg ggcccggggc gaacgatttt
     1501 ccggagcggt tcggtgaact cctcgccttc tccggcgacg ggttcccgga ggatcacccg
     1561 ttccggtccg tggtcgccat gcccaacgac atagagttgc cgccgatctg gctcctcggt
     1621 tcaagcgggt actcggccac tgcggcagga aagatgggcc tcggatacgc tttcgccgcg
     1681 catttcagcc cctcagaccc cgcccccgcc atgcacgcct accgagagaa cttcgagccc
     1741 tcgaagggct ttgcgcgtcc ttcggccgta ctcgcggttg ccgtcgtttg cggggagaca
     1801 gaagatcatg cgcggaggtt ggcctcctcc atggagctgg cctgggtccg catgcgtagc
     1861 gggagacccg ggccgctgcc gagccccgaa gaggcgatgg gcacttccta cacgccgggc
     1921 gaacgccggc tggccgatgc gtaccgctcg atgcagatag caggggatcc cgccagcgta
     1981 cgggcgcaga tcgagggtct ggccgagcgt acgcaggcag acgaggtcat ggtcaccacc
     2041 aacgtctacg accacgccga gcggctcggg tcctacgaga ggctcgccgc ggcattcgag
     2101 atggcggcac ccgccaggta ggccgccccg taaactatcc ggaagcgagg agacggcatg
     2161 gcagacgaga acccgcagca aggtggtctc aagggatcgt ccggcacccg ggaggtctac
     2221 cccgacggtt cgatcctcgg aggggagatc gggctctgga gttcggacct gaacgcgtta
     2281 cccgccgccg gggcgcaaga ggccgcggcg gagatagaag agcttggata cgcggcgctg
     2341 tggttcggcg agaccctcgg aagagaggcc ttcaccaacg cctcgatgct cctctccgcg
     2401 acgagccgcc tcgtggtggc gaccggcata gccaacatct tcgtccgcga tgcgtgggcc
     2461 acgaacgccg cggcaaggac gatcgcgggg gcgcaccctg gccggttcgt tctggggctg
     2521 ggcgtcagcc acaagcccat ggtccagatg cgcggccacg actaccgctc accgctgtcc
     2581 accatgcgcg cctacctgga ggacatgagc gaagcccagt tcacggccgc gcagcccgat
     2641 caggaaccgc cctgcctgct cgcggccctc ggacccagga tgctggagct ctccagagac
     2701 ctcgccgacg gcgcgcatcc ttacctggtg accccacagc acacggcgga ggcacgcgag
     2761 atcctcggag agggtccact gctcgccgtg gagcaggcgg tggccccgac ggaagaccgc
     2821 gaggaagcac tgcggttggc ccgctcccac ctgagccgct acctccagat gccgaactac
     2881 cgcaagagct ggcttcgcca gggtttccgg gaggaggacc tctcggggga aggcagcgag
     2941 gggctcgcca cggggctcgt ggcctggggc tcagaagacg aaatactcgg gcgggtgcgc
     3001 gagcatctct cggcgggtgc tgatcaggtt tgcgtacagg tgctgaccga cggtgtggaa
     3061 gacctcaggg gacggtggcg ctccctcgcc cccgtactac tcgggtaggc gccggcagag
     3121 gtcgtacgcc acgcaaccta tttcgttctg cctctcagcc tcccgcgttc atctctaccg
     3181 cgggcgtctc gatgcccaag aagccggggc taccactggc gtgcccggct cgcagctcct
     3241 cttgatccgg ggcccggtgc ctctcggaga tagataccat cgaaaaaccc tctacggtgc
     3301 cttcctccga actccggctg aacgacggca tacgaagttc gtcgggtcag cgtccaccct
     3361 tcaccgacct caggcgagca gagaagatgc tggcggagcc cgcggtttgc cgatctacgc
     3421 cgaatttctt cgttgcaaga tgtcgagaat tcgttgcttg ctccgaccta ccctcgagga
     3481 tcgcgcctcg cgggtgggcg aggtagaaca caatcccgtt tccgcgaccc gtacggatcg
     3541 cggacgacac gagggcaggg cagactagcc cgacacgccg caccagtcga cgagcaggca
     3601 ggcgatggtc tctgtcgccg tcagcaagtc ggttatggag atgtgttcgt cgggacagtg
     3661 ggcccagccg atatccccag ccccgtacat cacgcacggc attcctgcga agcgcacgaa
     3721 gaaacgcatg tccgcgccgt aggccgaccc ttctacgccc ggcctcttgc ccgtgacgcg
     3781 ctcgtgggcg tgggagacgg cctggcagat aggcgcgtcg accgggacct cgacgggtac
     3841 gctctggcca ccaggccact cgatctcggg cgggtgctcc cgtagccagg ggtccttttc
     3901 ggccgcttcc atgatgcggt ctgcgaccat ggatttcgag tcatcgagct cctcgccggg
     3961 gatcaggccg acccgacctt cagcgaagag cgtctcgggg acggtgatcg accactcgcc
     4021 cgcgcgcacc acgccgaggt tgatggcggc cctgtccggg acgccgtcgt agagtggatg
     4081 gcggagcttg gcctcgcgct cccgctctag ctccccgagc gcctcgaaga tcggcaagaa
     4141 cttctcgaac gccgagaccc cgcggtggcg cgccgaggcg tggaccgccc tgcccctcac
     4201 cgtgatacgg aagacgagcg agccggcctg cgccggtacg agccgcagtc gcgttggctc
     4261 cgtaaccacg gcggcgtcgg cccggtagcc cgcgagcacg gtcgcgaggg cgccgacgcc
     4321 tgtgtcctcc tccccgaccg tcgtcgccac ggtaacgtcg ccccgcaggt ccagcccgag
     4381 aacatcgagg gcctccaggg ccgagatcac ggtcgcgatg ccgcccttca tgtcgcaggc
     4441 gccacgcccg taaagcaggt caccgaccac ctcacccgag aacggcgggt aggtccacat
     4501 ggctgggtcc ccggggccca ccgtgtccac gtgaccgttc aagaggagcg agcgcccgcc
     4561 cccgctgccg gccctgacgc caacgatgtt gggcctgccc tctggcttgc tctcccagcc
     4621 gacgtgctcc gcgtagggcg cgacctgcgc cgtggtggcc tcgcagcgct cgaccgcgag
     4681 cccgcagccc aggagggcat cctcgacgac ctcctgaaca gcgacttcgc cgccgctggg
     4741 cgaggcgacg cgcaccagcc tctgcagcag gcccaccgac tcctcgcggc gcgccctgac
     4801 ggcgtgcccg atcctctcga aaatttccgc tttgtgttcg atctccaccc gcttctccta
     4861 tcaaaacccg cactcacagg aacaaacgac gcattatgga ggccctcagc ctccggtcaa
     4921 gtcttctggc cgcggcctcc gccctgccgt gttgacccgg ttgggccggt cgtttagcat
     4981 cgaagtcgtt ctcggtgtcg acgcggctct gccgtgaaag gtggtcgctc gcatggacgt
     5041 gatggatggt ctcgggaagc gcggagacag gacgaagctt gccggtgttg ataacctgat
     5101 gctccacttc acgccttacg ctgaggattg gtcgaagcta ccggtcatcg tttccgggga
     5161 cggctcttac gtaaccgacg acaagggtag gacctacata gacggtttgg ccggcctcta
     5221 caccacgcag gttggccacg gcaggacgga gcttgcggac gcggcccaca agcaaatgaa
     5281 ggagctaggc ttcttcccaa actggagctt ccagcacccg agaagcctgg agcttgcggc
     5341 caagatcgcc gagatcgcac cgggggatct caactccacc ttcttcgtct cctccggttc
     5401 tgaggcggtt gagaccgtca tcaagctggc caggcagtac cacaaggcca acggcgagcc
     5461 gggacggtac aagatcatct ccagggacgt ggcctaccac ggcaccacga tgggcgcgct
     5521 ctccgtgacg gggcttcccg gcttcaaggc ccccttcgag ccgctgccct ccggcttctt
     5581 ccacgtcagg aacacccagc aggaccccga aggcgctgcc gacgccatcg agcagatgat
     5641 agagacggaa ggcccggagc tggtcgccgc cgtgatcctg gagcccgtgc agaacgccgg
     5701 aggctgcctg gttccgccgc ccgactactg gaagagggtg cgtgagatct gcgacaagca
     5761 tggcgtcctg ctcgtctccg acgccgtgat ctgtgctttc ggcaggctgg gcgagtggtt
     5821 cggcatcgag cgcttcggcg tggtgcccga catgaccagc ttcgcaaagg gcgtcacgag
     5881 cggctacctt cccatgggtg gggtcgttgt gaacgaccgc atcgcacaca ccctcaagga
     5941 cagggcgccg atgttcatgc acggctccac gttcggtggg cacccggtct cctcggcggt
     6001 ggccctggag aacatagcga tcatggagcg cgagaacctg ctgcagaacg tccacgacct
     6061 cgaaggccac ttcggggacg agctgaagaa gatggc
//
LOCUS       DBNGVD010000076         3411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2567032, whole genome shotgun sequence.
ACCESSION   DBNGVD010000076 DBNGVD010000000
VERSION     DBNGVD010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2567032"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="AC16CM_02235"
     CDS             complement(<1..899)
                     /locus_tag="AC16CM_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3138673.1"
                     /translation="MERETENRFFGPNLGYVLELYEQFKEDPDAFDGPTREFFETWTP
                     PRVEMPSANGQAVAPEVDVEKAVGASKHVRHIRDFGHRAAKLDPLGSEPPGDPTLDPT
                     FYRITDADLESLPTLIIGGPVAGRTSNAREAVEELRKLYCHTTGYDFGHVHDPVERFW
                     LRESVESGRFYKRMEGESAKRLLERLTRVDAFEKFLHKTFLGQKRFSVEGNDMVVPML
                     NRIIRSAADVGTPVATLGMAHRGRLNVLAHVMDKPYSKIFGEFQQPDKGEQPSAAGSR
                     GDAWVGDVKYHLGIRGFQLGDEEA"
     gene            1166..1483
                     /locus_tag="AC16CM_02240"
     CDS             1166..1483
                     /locus_tag="AC16CM_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3138674.1"
                     /translation="MDDRIDYFERMLANNPDNPTGLLALANEYQKAGREEDEAAVLQR
                     YLDVQPDDEGNAYARLGSVLSNLGRRDEARSAYEKGISQSERHGHSGMADDLRLALIG
                     LNE"
     gene            complement(1654..2883)
                     /locus_tag="AC16CM_02245"
     CDS             complement(1654..2883)
                     /locus_tag="AC16CM_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138675.1"
                     /translation="MADGVEVQKQVDGRIAVLAWGMCVLALALIFVSLMLTLLGTSRI
                     VGLDFPLVGVSGALVGGLVASRKPRNPVGWFFLAGACIGGLQALAGTYAVYGLIVDPG
                     SVPFASFSAWMTRTLQIAGPIFGFVLVPLYFPTGRPPTPRWRVITWTTLGVLPLATAL
                     MAFSPGETVYNTGIPNPLAVEALQPVNEALRPFVFASYIGLMLAAAASLVVRLVRSRG
                     GERKQIEWFVFAAALVPLWFLVNSPVEQASPVLFRVLDALIIAGVPVAAGIAILRYRL
                     YDIDRIINRTIVYTALTAVLVAVYFGAIVLSQQVLIFLTGQRSTLAVVVSTLVIAALF
                     NPMRRRTQSFVDRRFYRSKYDARKTLETFSTKLREETDLGALNDELIWVVRETVQPAH
                     ISLWLRPEDAPEEATQG"
     gene            2969..>3411
                     /locus_tag="AC16CM_02250"
     CDS             2969..>3411
                     /locus_tag="AC16CM_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3138676.1"
                     /translation="MTDPRHFDTRAVHSGEHRASVEEGTDGTFYPISTPIYASTTFSH
                     PSIETTDRILGGEEQGYSYARYDNPTVRAFEEAIVSLEAPGSGGGIRAFAFGSGMAAT
                     HAALASAELTNGATVLAAEQLYGSTATLLVQIFGANGVQTRFVDA"
BASE COUNT          651 a         1115 c         1094 g          551 t
ORIGIN      
        1 gcctcctcgt cccccagctg gaaacccctg atgcccaggt gatacttcac gtcaccgacc
       61 caggcgtcgc cacggctgcc cgcggcggag ggctgctcgc ccttgtccgg ctgctggaac
      121 tcaccgaata tcttggagta aggcttgtcc atcacgtgcg cgagcacgtt gagcctgccg
      181 cggtgggcca tgcccagcgt ggccaccggc gtgcccacgt cggccgcgga gcggatgatc
      241 ctgttcagca tcgggacgac catgtcgttc ccctccacgg agaaccgctt ctggccgagg
      301 aaggtcttgt gcagaaactt ctcgaaggcg tcgacccgcg tgagccgctc gagaaggcgt
      361 ttggcgctct ccccttccat ccgcttgtag aagcgccccg actccacgga ctcgcgcagc
      421 cagaacctct caacggggtc gtggacgtgc ccgaagtcgt agccggtggt gtggcagtag
      481 agcttccgca gctcctcgac cgcctccctg gcgttcgagg tgcgcccggc gacggggccg
      541 ccgatgatga gggtcggcag cgactccaga tccgcgtcgg tgatgcggta gaaggtgggg
      601 tccagcgtcg ggtccccggg cggttcgcta cccagcgggt cgagcttggc cgccctgtgc
      661 ccgaagtccc ttatgtgcct gacgtgcttg gaggccccaa cggccttctc cacgtccacc
      721 tcgggtgcca ccgcctgccc gttggccgaa ggcatctcca cccgtggcgg ggtccaggtc
      781 tcgaagaact cccgcgtcgg cccgtcgaag gcgtcggggt cctccttgaa ctgttcgtag
      841 agttccaaca cgtagccgag gttcggcccg aaaaatctgt tctccgtctc tctttccaac
      901 cggatcacac cctcatgtca cgataccagt aatggtccga acgccgcgcg aagaggccag
      961 gcatccgatg gtgatgccct ccaccgacgc tcttcgcaag acgatcaaac atcttgatct
     1021 ttcctatgat accccacatc gtctgctacg taccccgtct ctgcgcccca aaacacctcc
     1081 gaattctacc cccaaaaagg ggcgagcacc gtcggaacac accctgtcct atactgaccg
     1141 cgtcgatagg gtaagaaagg attccatgga cgacaggata gactacttcg agcggatgct
     1201 ggccaataac ccggacaacc cgacggggct gctggcgctg gccaacgagt accaaaaggc
     1261 ggggcgcgag gaggatgagg ccgcggtcct gcaacgctac ctggacgtcc agcccgacga
     1321 cgaaggcaac gcctacgccc gcctgggcag cgtgctctcc aacctgggac gcagagacga
     1381 ggcgcgcagc gcctacgaga aaggcatctc ccagtcggag aggcacggtc atagcgggat
     1441 ggccgacgac ctcaggctcg ccctgatcgg gctgaacgaa taggatcccg gcaccccgtg
     1501 gttcgcgtcc cggagaatgt tgtccgtcgt aaccggatcg gtacacggct gttaccaacc
     1561 cttaactctt ttgaccggat aatgcgcgca agccctccgg cgaagtaggg gatccccctc
     1621 cgcttgcaca acacgccggg gggtatcttt ttgtcagccc tgggttgctt cttcgggggc
     1681 gtcttcaggg cgtaaccaca gggaaatgtg ggctggctgc acggtctccc tgaccaccca
     1741 gatcagctca tcgttcaggg ccccgaggtc tgtctcctcg cgcagtttcg tcgagaaagt
     1801 ttccagtgtc tttctggcgt cgtacttgct gcggtagaag cgacggtcca cgaaggactg
     1861 ggtgcgcctc ctcatgggat tgaagagggc ggcgatcacc agggtcgaga cgaccacggc
     1921 aagggtggac cgttgtccgg tcaggaagat gagcacctgc tgcgagagta cgatggcgcc
     1981 gaagtagacg gccacgagca cggccgtcag cgcggtgtag acgatggtgc gattgattat
     2041 gcggtctatg tcgtagaggc gatagcgcag gatcgcgatg cccgcggcaa ccggaacgcc
     2101 ggcgatgatg agggcgtcga gcaccctgaa caggaccggc gaggcctgct ccacgggtga
     2161 gttgaccagg aaccacaggg gcaccagcgc ggcggcgaag acgaaccact ctatctgctt
     2221 cctctcccca ccccgggagc gcaccaggcg gaccaccagg ctcgccgcgg cggcaagcat
     2281 gaggccaatg tagctggcga agacgaaagg acggagggcc tcgttcaccg gctgcagggc
     2341 ctcgacggcc agggggttgg gtatgcccgt gttgtagacc gtctcgcccg gcgagaacgc
     2401 cataagggcg gtagccaggg gcagcacgcc gagcgtggtc caggtgatca cccgccagcg
     2461 cggcgtgggt ggacggcctg tggggaagta cagcggcacc aggacgaaac cgaagattgg
     2521 cccggctatc tggagcgtcc tggtcatcca ggcgctgaag ctggcgaagg gtaccgagcc
     2581 gggatcgacg atcagaccgt agacggcgta ggttccggcc agcgcctgaa gcccgccaat
     2641 gcaggcgccg gctagaaaga accatccgac cgggttgcgt ggcttgcggg aggcgacgag
     2701 gccgccgacc agcgccccgg agaccccgac gagcggaaag tcaagcccga cgatgcggga
     2761 ggtgccgagc agggtcagca tgagggagac gaagatcagg gcaagcgcga gcacacacat
     2821 gccccaggcc agaaccgcga tccggccatc gacctgcttc tgcacttcta ctccatcagc
     2881 caccacacca ccctatccgg gaccatcgac cgcccgcgaa tccggagcct cgccaaaggc
     2941 gcgtcccggt aggttagtat aggcgaccat gactgatccg agacacttcg atacccgtgc
     3001 cgtgcactcc ggggagcaca gggcaagcgt ggaagagggt actgacggca ccttctaccc
     3061 gatctcgacg cctatctacg cctcgaccac gttctcccac ccgagcatag agaccaccga
     3121 ccgaatactc ggcggcgagg agcaggggta cagctacgcc cgctacgaca acccaaccgt
     3181 ccgcgccttc gaggaggcga tcgtctcgct cgaggccccc gggtcggggg gtgggatcag
     3241 ggccttcgcc ttcggctcgg ggatggcagc cacccacgcg gcgctcgcct ctgcagagct
     3301 gacgaacggg gccacggtcc tcgcagcgga gcaactctac ggctcgacgg cgacgctgct
     3361 ggtgcagatc ttcggcgcga acggggtcca gacgcgcttc gtggacgcct a
//
LOCUS       DBNGVD010000077        14811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_198507, whole genome shotgun sequence.
ACCESSION   DBNGVD010000077 DBNGVD010000000
VERSION     DBNGVD010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_198507"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /gene="rplB"
                     /locus_tag="AC16CM_02255"
     CDS             <1..244
                     /gene="rplB"
                     /locus_tag="AC16CM_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565111.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3138677.1"
                     /translation="HQNVRWGKAGRKRHLGIRPTVRGTVMNPVDHPHGGGEGKSTPGR
                     APVTPWGKITQGSPTRKKHKRSDAMIVRRRRKGRRR"
     gene            245..526
                     /gene="rpsS"
                     /locus_tag="AC16CM_02260"
     CDS             245..526
                     /gene="rpsS"
                     /locus_tag="AC16CM_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3138678.1"
                     /translation="MTRSAKKEPFVEERLMERILRMNENNEKRMLKTWSRASVIYPEF
                     VGHTIAVHDGRKHVPVYCTESMVGHKLGEFAPTRTFRTHVKGDRLARRR"
     gene            523..888
                     /gene="rplV"
                     /locus_tag="AC16CM_02265"
     CDS             523..888
                     /gene="rplV"
                     /locus_tag="AC16CM_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3138679.1"
                     /translation="MKAKARYVRIAPRKARLVADEVRGKSVPEAVSLLAFTNKRAAKI
                     IAEVIDSAAANAEHNDDADPDELFVRDIRVDDGPTIKRYRARAMGRATMIRKRTSHIS
                     VELGAPAGVLVGTPGDEEE"
     gene            889..1521
                     /gene="rpsC"
                     /locus_tag="AC16CM_02270"
                     /pseudo
     CDS             889..1521
                     /gene="rpsC"
                     /locus_tag="AC16CM_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            1561..1980
                     /gene="rplP"
                     /locus_tag="AC16CM_02275"
     CDS             1561..1980
                     /gene="rplP"
                     /locus_tag="AC16CM_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3138680.1"
                     /translation="MLVPKKLKYRKPHRGRMRGQAKGGTDVQFGEYGLQALEPAWITN
                     RQIEAARIAMTRKIRRGGKVWINIFPHKPVTKKPAETRMGSGKGSPEAYVAVVKPGRV
                     MFEMSGVEEELAREAMHLAAQKLPIKTRFMARGGGQQ"
     gene            1977..2174
                     /gene="rpmC"
                     /locus_tag="AC16CM_02280"
     CDS             1977..2174
                     /gene="rpmC"
                     /locus_tag="AC16CM_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3138681.1"
                     /translation="MKVAEVRELDVEELEQRLATTRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNHKQQESQ"
     gene            2333..2599
                     /gene="rpsQ"
                     /locus_tag="AC16CM_02285"
     CDS             2333..2599
                     /gene="rpsQ"
                     /locus_tag="AC16CM_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3138682.1"
                     /translation="MQTDSERNRRKERVGLIVSDKADKTVTVAVEALVRHPMYKKRVR
                     RSKKFIVHDEANEARVGDTVRIIETRPLSARKRWRLANVISRAE"
     gene            2600..2968
                     /gene="rplN"
                     /locus_tag="AC16CM_02290"
     CDS             2600..2968
                     /gene="rplN"
                     /locus_tag="AC16CM_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3138683.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRTAGVGDVIVATVK
                     EATPGSAVKKGEVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            2969..3286
                     /gene="rplX"
                     /locus_tag="AC16CM_02295"
     CDS             2969..3286
                     /gene="rplX"
                     /locus_tag="AC16CM_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3138684.1"
                     /translation="MGLKIKRGDTVVVVSGKEKGKRGEVEHVLPRENRVVVNGVNVRT
                     RHARPSQQNQQGLYHFEAPIDVSNVMLIDPTDGEPTKVGYRFTDSGEKVRVGKKSGKD
                     IDG"
     gene            3279..3821
                     /gene="rplE"
                     /locus_tag="AC16CM_02300"
     CDS             3279..3821
                     /gene="rplE"
                     /locus_tag="AC16CM_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3138685.1"
                     /translation="MARMRDRYKEEVAPNLKERFGIENPMRMPGLEKIVVNMGVGEAV
                     VNSRAMDGAMEDLARITGQKAQLRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGVSPAAFDGHGNYAIGLREQVIFPEISYDSIDATRGLDVAIVTTT
                     ESDEEARELLRLLGMPFRQS"
     gene            3831..4016
                     /locus_tag="AC16CM_02305"
     CDS             3831..4016
                     /locus_tag="AC16CM_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3138686.1"
                     /translation="MTRKALLEKQQKKQKFAVREYSRCQRCGRSRGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            4023..4421
                     /gene="rpsH"
                     /locus_tag="AC16CM_02310"
     CDS             4023..4421
                     /gene="rpsH"
                     /locus_tag="AC16CM_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3138687.1"
                     /translation="MSVNDPIADFLTRVRNAHMAKHETVEIPHSKMKAEIARILQEEG
                     YVQEVSERGSGVERRLVIALKYGPDGRRGITGLRRMSRPGRRVYRKQTNIPRVLDGLG
                     VAILSTSQGVLTDHQARRRGVGGEVLCFVY"
     gene            4436..4969
                     /gene="rplF"
                     /locus_tag="AC16CM_02315"
     CDS             4436..4969
                     /gene="rplF"
                     /locus_tag="AC16CM_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3138688.1"
                     /translation="MSRIGKAPVEVPGAVSVELSGRSVKVNGPKGDLTVPVGRGVEVR
                     QEDGSIVVDRASDSPEHRAMHGLTRSLIQNAVTGVTDGFTRTLNIAGVGYRAALQGQD
                     LNLQVGYSHPVSIAPREGIQFEVPNATTIIVRGIDKQRVGQTAAEIRMVRPPEPYKGK
                     GIRYSDEQVRRKVGKAG"
     gene            4989..5339
                     /gene="rplR"
                     /locus_tag="AC16CM_02320"
     CDS             4989..5339
                     /gene="rplR"
                     /locus_tag="AC16CM_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3138689.1"
                     /translation="MATLQKRMHREKRRKRVRRKVVGSADRPRLSVYRSNVHIYAQLI
                     DDDAQVTLAAADSREVGAAESRKEAARKVGELIASRASEAGIETAVFDRGGNKYHGRI
                     AALAEGAREAGLKL"
     gene            5360..5908
                     /gene="rpsE"
                     /locus_tag="AC16CM_02325"
     CDS             5360..5908
                     /gene="rpsE"
                     /locus_tag="AC16CM_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3138690.1"
                     /translation="MGRQRNDNRQGGRGQGGGGRGGRQRDASDLQDRVVEIRRVAKVV
                     KGGRRFNFSALVVVGDGKGRVGAGLGKANTVPAAIQKGQDKAKRNMFNVPMRNTTIPH
                     EVIGKFESSSVLLKPASEGTGVIAGGGVRAVLELAGVRDVLTKALGSTTSVNLVKATV
                     EGLQRLRSKAEIEEMRGVKITL"
     gene            5905..6087
                     /gene="rpmD"
                     /locus_tag="AC16CM_02330"
     CDS             5905..6087
                     /gene="rpmD"
                     /locus_tag="AC16CM_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3138691.1"
                     /translation="MSPLKVTQVRSVIGSKAGHKKTVRALGLKRIRDSRVHEDTPQIR
                     GMVHKVQHLVEAEEVD"
     gene            6090..6551
                     /gene="rplO"
                     /locus_tag="AC16CM_02335"
     CDS             6090..6551
                     /gene="rplO"
                     /locus_tag="AC16CM_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3138692.1"
                     /translation="MRLNDISPAPGSTRARKRVGRGTGSGHGKTSGRGHKGAGARSGA
                     SAFAGYEGGQMPLQRRLPRLKGEARGRHTVARPTVYAPVNLGELRDVAGDTIGPDELR
                     AAGLVRKKAGLVKILGNGDIGRAVTVRAHAFSRSAQEKIEAGGGSVEVLEH"
     gene            6554..7834
                     /gene="secY"
                     /locus_tag="AC16CM_02340"
     CDS             6554..7834
                     /gene="secY"
                     /locus_tag="AC16CM_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3138693.1"
                     /translation="MLGSLANAWKIPELRTRLMFTFAMLAAYRLGAYIPLPGVRPEVL
                     GGGLQTSNPVTGLFGTFTGGAFNNFAVFSLGIMPYITAAIVMQLMTVAIPRLQELAKE
                     GETGQQKITQYTRYFTLALALVQSVAMVFFFRSAQGGGALAGTTPVEILVAVVTLTTG
                     VMLTMWFGELISQRGLGNGISLIITASILSQAPLALQALRENGDVLTVVILGLLAVLI
                     IAAIVFVNEGQRRIPITYAKRQVGRKMSQGGTTYLPLKVNMAGVIPIIFASSLLLFPV
                     VLGQLASRGDPNSFLGRLSQFFDPGNAPYLILYALFIIMFTYFYTAVQFNPVEHADNL
                     KKSGGYVPGYRPGQPTAIYLNNVLTRITLFGALFLAVVAVLPYFISDALGLGTSIFLG
                     GTSMLIVVGVSLDTVRQLESQLMMRNYEGFLKRR"
     gene            7831..8469
                     /locus_tag="AC16CM_02345"
     CDS             7831..8469
                     /locus_tag="AC16CM_02345"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3138694.1"
                     /translation="MRVILLGPQGAGKGTQAKRLADRVGAKHISTGDIVRAEIESGSE
                     LGRKVQEYNDKGELVPDEIIVEMAKPHLEDAGAWLLDGFPRNEAQAQALDTALEEIGE
                     DLDAVVALEAPDEDLVERLSGRRQSQATGKIYHVDYDPPPEDGDDPGPFVQRDDDAED
                     AIRRRLQIYHDQTEPLKDYYAGRDLLVTVDAAQEIPKVTEDVLEAVGWREDA"
     gene            8466..9242
                     /gene="map"
                     /locus_tag="AC16CM_02350"
     CDS             8466..9242
                     /gene="map"
                     /locus_tag="AC16CM_02350"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3138695.1"
                     /translation="MIVRKSRKELEAMREGGRITAACLEMLKENVRPGVTTGELDRLA
                     EDFIRSNGGTPEFKGYPGSPGAPDFPGSICASPNAMIVHGIPGSYRLKSGDIISLDVG
                     VRYQGFVTDSARTVPVGDVPDDVAGLLRITQECLEAATEQMRSGNRLGDVGYAIQSTA
                     ESKGYGVVRDLVSHGVGRQMHEDPQIPNYGNPGTGPRLKPGMTFAIEPMITLGDYSIR
                     ISEWDGWSIYTADGSLSAHFENTIAVTENGPWVLTQNGAP"
     gene            9344..9565
                     /gene="infA"
                     /locus_tag="AC16CM_02355"
     CDS             9344..9565
                     /gene="infA"
                     /locus_tag="AC16CM_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3138696.1"
                     /translation="MAKEDVIEVEGTVTEALPNTQFRVELENGHNVLAHISGKMRMNY
                     IRILPGDRVKVELSPYDLSRGRITYRFRT"
     gene            9578..9691
                     /gene="rpmJ"
                     /locus_tag="AC16CM_02360"
     CDS             9578..9691
                     /gene="rpmJ"
                     /locus_tag="AC16CM_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3138697.1"
                     /translation="MKVRASVKPICERCKVIRRRGVVRVICTNPRHKQRQG"
     gene            9693..10064
                     /gene="rpsM"
                     /locus_tag="AC16CM_02365"
     CDS             9693..10064
                     /gene="rpsM"
                     /locus_tag="AC16CM_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3138698.1"
                     /translation="MARIAGVDLPREKRVEVGLTYIYGVGRSTARKICRDTEVDPDTK
                     VRDLAEGEVGKIRTYIDQNVEVEGDLRRETQQNIRRLIDIGTYRGIRHRRGLPVRGQR
                     TKTNARQRKGPKPSIGGRKKR"
     gene            10065..10484
                     /gene="rpsK"
                     /locus_tag="AC16CM_02370"
     CDS             10065..10484
                     /gene="rpsK"
                     /locus_tag="AC16CM_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3138699.1"
                     /translation="MGRQRQQRQRQRGGASRSRRRVRRNVTTGVVHIKSSFNNTIVSV
                     SDQEGNVISWESAGSLGFKGSRKSTPFAAQMTAESVANKAMDQGVRRVDIEVKGHGSG
                     RDMAARTFQSMGIEVLSIKDVTGQPHNGCRPPKRRRG"
     gene            10488..11090
                     /gene="rpsD"
                     /locus_tag="AC16CM_02375"
     CDS             10488..11090
                     /gene="rpsD"
                     /locus_tag="AC16CM_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3138700.1"
                     /translation="MARYTGPRGRRDRRAGVMLSSMRKNPLEKKPYPPGEHGRGRHRQ
                     TEFGMRLMEKQKARWYYGVSEKQFRRAYDRATRMQGVSGENLLRLMELRMDNVVFRLG
                     FATSRPQARQLVVHGHFQLNGRKHNVPSAILKPGDVITVKEKSRQLEPIQNAVENVVA
                     VPPWLEADHDNFTGRLLHTPARDEIDTPVEEQLIIEFYSR"
     gene            11126..12070
                     /locus_tag="AC16CM_02380"
     CDS             11126..12070
                     /locus_tag="AC16CM_02380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3138701.1"
                     /translation="MLDIAPPRFRVEEEETRRGTFVAEPLPRGLGHTLGNSLRRVMLS
                     GLTGAAVTKIRIEGVSHEFSTIEGVKEDVVDIILNIKGLKFKLEREEPIELTIAKDGP
                     GEVTAADIELKADVEIVDPEAYIASVSDGGHLDMRLTVERGQGYARAEQNKSDADPIG
                     VIAVDSLFSPVQRVNYTVTETRAGARADLDSLRMEVFTDGRIEPQEALQDASRQLMDF
                     LGLFTDGYRGAGAPDPNQRGGRPVITDERPVEDLELTVRSYNCLKREGVDTIGQLATM
                     TEEELMNIRNLGMKSVDEIRSKLLEYGYSLESEGNEAR"
     gene            12057..12422
                     /gene="rplQ"
                     /locus_tag="AC16CM_02385"
     CDS             12057..12422
                     /gene="rplQ"
                     /locus_tag="AC16CM_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3138702.1"
                     /translation="MRHAKRGRKLGREAQHRNLMLGTMAGQVITHGRIKTTAPKAKEL
                     RGVVDKLINTAKKDDLASRRQAVKVLKDKTVVRHLFEDVAPDLDDRNSGYTRILKLGP
                     RLGDGAETVYLELVNYSTE"
     gene            12490..13287
                     /locus_tag="AC16CM_02390"
     CDS             12490..13287
                     /locus_tag="AC16CM_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3138703.1"
                     /translation="MTFYYPGATDPALSEVGFELRPGEYVGVVGPNGGGKSTLVRLLN
                     GLLRPDSGRVRVSGHDPATEPFEVRKRLGILFQNPENGLVAPFVEDDVAFGLENLGVP
                     REQMRERVAGAIRAVGLEGYERREPHTLSGGEKQRVALAGLLAVEPEILVLDEPTSML
                     DAAGRREVLERLETLRSRKTVLHVTHHLEELLHADRVLVLNGGELVADESPQRLLSDP
                     GLLQENRLILPVVPRLAQALGLRADLGTPEELAEAILTGTGSGTRSG"
     gene            13284..14099
                     /locus_tag="AC16CM_02395"
     CDS             13284..14099
                     /locus_tag="AC16CM_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3138704.1"
                     /translation="MRLELRDVSHVYAAGTPFEVEALRGVSLTVEPAEVLGIIGGTGS
                     GKSTLVRHMNLLLAPADGEVLVDGVDATTLKKSELRRRVGLVFQFPEAALFAPTVGED
                     VAFAPRHLGLDEEEVGERVRGSLGLLGVGDLAGRSPFALSGGERRRVAIAGVLAMGPE
                     VLVLDEPTAGLDPATRRELLALIRRLREAGVSVVLVSHDLDEVAEVADRVCALHDGSV
                     RSIGSPAEVFYAEPDQAPATVRVASLLRASRPDIGEPVRFDETVAALKALPGV"
     gene            14101..>14811
                     /locus_tag="AC16CM_02400"
     CDS             14101..>14811
                     /locus_tag="AC16CM_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3138705.1"
                     /translation="MTSLGQFYPVASPVHALDPRAKIVATAILVAGLFLVDSIVGLLF
                     VAAALVALVAASRIPARDFLRLLRPVLFIVALTVVFQLLFSREGATIFELGFLEVHTG
                     GLRLAFFLALRILLLVGIAGVLTATTAPVALADGIEDLLSPLKKVRFPAHEVAMMMTR
                     ALRFIPTLGEEAEKIRGAQAARGADFTEGGPLRRARSLVPVLVPLTVGAFRRADELAE
                     AMESRGYKGGEGRTRYREL"
BASE COUNT         2900 a         4568 c         5155 g         2188 t
ORIGIN      
        1 gcaccagaac gtcaggtggg gcaaggccgg ccgcaagagg cacctcggca tcaggccgac
       61 ggtgcgtggt acggtcatga accccgtgga ccatccgcac ggcggtggcg agggcaagtc
      121 cacgccgggc cgggcccccg ttacgccgtg gggcaagatc acccagggct cccctaccag
      181 aaagaagcac aagcgttcgg acgccatgat cgtgcgtcgg cgcaggaaag gcaggaggcg
      241 ctagatgacg cggtcagcca aaaaagaacc gttcgtggag gagcggctca tggagcgcat
      301 cctccgcatg aacgagaaca acgagaagcg catgctcaag acgtggagcc gggcgagcgt
      361 gatctacccc gagttcgtcg gccacaccat cgccgtgcat gacgggcgca agcacgtgcc
      421 cgtctactgc acggagtcga tggttggcca caagctcggc gagttcgccc cgacccgtac
      481 cttccgtacg cacgtcaagg gcgaccgtct ggcgaggcgc cgatgaaggc gaaggccagg
      541 tacgtgcgca tcgccccgcg caaggcgcgg ctggtggcag acgaggtcag gggcaagagc
      601 gttcccgagg ccgtctcgtt gctcgcgttc accaacaagc gggccgccaa gatcatcgcc
      661 gaggtgatcg acagcgccgc cgccaacgcg gagcataacg acgacgcgga ccccgacgag
      721 ctgttcgtgc gggatataag ggtggacgac ggccctacca tcaagcgcta ccgcgcgagg
      781 gccatgggcc gggccaccat gatccgcaag cgcaccagcc acatcagcgt cgagctcggg
      841 gcaccggctg gcgttctggt gggcacgccc ggagacgagg aggaatagat gggccagaaa
      901 gtacatcccg aaggcttccg tctcggcgtt cagcgcaggg gcgagaaggt agatttcaag
      961 agcagctggt actcggacag ggacttcgcc gagcttctcg gcgaggatct caagatccgg
     1021 gagcacatcg agaagaagct caccagggcc ggaatcgccg acatccagat ccgcaaggcc
     1081 gccgagcagg gtgagatcgc cgttgacatc cacaccgcga ggccaggcat cgtgatcggc
     1141 aagggcggca gcgaggtcga cgccctgcgc ggcgagctcg agcgcctcac cggcaagaag
     1201 gtgcaggtca acgtcaggga ggtctcgagg cctgagctca acgccagcct cgtcgccgag
     1261 tccatagcgg agcagctcgt gggacgggcc gcgttccgga ggaccatgaa gcgggccctc
     1321 accagcgcca tccgcagcgg tgcggagggg gcgaggatcc agtgcggcgg caggctcggc
     1381 ggcatcgaga tgagccgctc ggagaccatc tccgagggtc gggtgccgtt gcacacgctc
     1441 gatgcggata tagattacgg cttcaaggaa gccaagacgc agatgggcca gatcggggtc
     1501 aaggtctgga tcaaccacgg cgtccacgac gagcttcccg aagcggtcag gcggtaggag
     1561 atgctggtac ccaagaagct aaagtacagg aagccccaca ggggcaggat gcgcggccag
     1621 gccaagggcg ggaccgacgt gcagtttggc gagtacgggc tgcaggcgtt ggagccggcc
     1681 tggatcacca accgccagat agaggccgcc cgtatcgcga tgaccaggaa gattcgccgc
     1741 ggggggaaag tctggatcaa catctttccc cacaagccgg ttacaaagaa gccggccgag
     1801 acccgtatgg gtagcggcaa ggggagcccc gaggcatacg tcgccgtggt caagccgggg
     1861 cgtgttatgt tcgagatgag cggcgtggag gaagagttgg cccgtgaggc catgcacctc
     1921 gccgcccaga agctccccat caagaccagg ttcatggctc gagggggtgg tcagcagtga
     1981 aggtggccga agtgcgcgag ctggacgtcg aggagctgga gcagcgcctc gccacgacga
     2041 ggcgtgagct cttcaacttg cgattccagc atgccacggg gcagttggag aacacgggac
     2101 agctcaaaga ggtcaggaag aacatcgcca ggatcctgac ggtcctgaac cacaaacagc
     2161 aggagagtca gtagtggtag aggaagagaa aaaccagacc ccggagacgg aggagcctgt
     2221 ggccgagacg ccgcaggacc tcgtcgaggc cggggaagcc ggggagaccg tcgaggccgg
     2281 tctcgacctc ggtccgtcca agggccccgc gctcgaccag gacgagggcc agatacagac
     2341 cgactccgag cgcaaccggc gcaaggagcg ggtcggtctc atcgtctcgg acaaggccga
     2401 caagaccgta acggttgccg tcgaggccct ggtacgtcat cccatgtaca agaagcgggt
     2461 gcgccggtcc aagaagttca tcgtgcacga cgaggccaac gaggcgcggg ttggcgatac
     2521 ggtcaggatc atcgagacca ggccgctctc ggcgcgcaag cggtggcggc tcgccaacgt
     2581 gatatcgagg gccgagtaag tgatacagca ggaatcgagg ctgagggtcg cggacaactc
     2641 gggggctagg agcatcctga ccatccgcgt gctcggcgga agcaagaggc gcaccgcggg
     2701 cgtcggcgac gtcatagtgg caaccgtcaa ggaggccacc cccggcagcg cggtcaagaa
     2761 gggcgaggtc gtgcgcgccg tcgtggtgag gaccaagaag gagacgcgcc gcgacgacgg
     2821 gtcctacatc cgcttcgggg agaacgccgg ggtaatcatc aacccggacg gcgccccgag
     2881 ggggacgcgt atcttcgggc ccgtggctcg cgagctcagg gagaagggct acaccaggat
     2941 catctcgctc gccccggagg tgttgtagat gggtctcaag ataaagcgcg gagacaccgt
     3001 ggtggtggta tccggcaagg agaagggcaa gcgcggcgag gtcgagcacg ttcttcccag
     3061 ggagaacagg gtcgtggtaa acggcgtcaa cgtacgcacg cgccacgcgc ggccaagcca
     3121 gcagaatcag caggggctct accacttcga ggcccccata gacgtctcga acgtgatgct
     3181 gatcgacccg actgacggcg agcctacaaa ggtaggctac cgcttcacgg actcggggga
     3241 gaaggtccgg gtcggcaaga agtcagggaa ggatatcgat ggctaggatg agggacaggt
     3301 acaaagagga ggtcgccccg aacctcaagg agcggttcgg tatcgagaac cccatgcgga
     3361 tgcccggctt ggagaagatc gtggtcaaca tgggcgtcgg ggaggccgtg gtcaacagcc
     3421 gcgccatgga cggcgcgatg gaggacctcg caaggatcac cggccagaag gcccagctgc
     3481 ggcgggccag gaagtccata gccgggttca agatccgtga gggcatgccc gttggcgcca
     3541 gggtcacgct gcgcggcgag cgcatgtggg agttcctcga ccgcttcatc tccatagccc
     3601 tcccgcgcgt cagggacttc aggggcgtca gccccgccgc cttcgatggg cacgggaact
     3661 acgccatcgg tctcagggag caggtgatct ttccggagat ctcctacgac tccatagacg
     3721 cgacgcgcgg cctcgacgtg gcgatcgtga cgacgacgga gagcgacgaa gaggcgcggg
     3781 agctcttgag gctcctcggg atgccgttca ggcagagctg agaggtaggt atgacgagaa
     3841 aagcactgct ggagaagcag cagaaaaagc agaagttcgc ggtcagggag tacagccggt
     3901 gccagaggtg cggccggtcg cgcggctact acaggaagtt cggcctgtgc cggatctgcc
     3961 tcagggagct ggcccacgag gggaagatcc ctggcgtgac gaaagcgagt tggtaggtaa
     4021 ttatgtcggt taatgatcct atagcggatt tcctgacgcg cgtgcgcaac gcgcacatgg
     4081 ccaagcacga gacggtcgag atcccccact ccaagatgaa ggcggagatc gcccgcatcc
     4141 tgcaggagga gggctacgtc caggaggtct ccgagcgtgg cagcggtgtg gagcggaggc
     4201 tcgtgatcgc gttgaagtac ggcccggatg gccgccgtgg tatcaccggc ctgaggcgga
     4261 tgagccgccc cggacgccgc gtctaccgca agcagaccaa catcccccgc gtgctggacg
     4321 gcctcggggt ggcgattctc tcgacctccc agggggtcct gaccgaccac caggcaaggc
     4381 gcaggggcgt cggcggcgaa gtactttgct tcgtctacta gggaggttct agaacgtgtc
     4441 gaggatagga aaagcccccg tggaggttcc aggcgcggtg agcgtggagc tctcgggccg
     4501 aagcgtgaag gtgaacggcc ccaagggcga cctcacggtt cccgtgggcc gcggggtcga
     4561 ggtcaggcaa gaggacggct ccatcgtggt agatcgcgcc tccgactcgc ccgagcaccg
     4621 ggcgatgcac gggctgactc ggtccctgat ccagaacgcc gtaacgggcg tgactgacgg
     4681 gttcacgagg acgctgaaca tcgccggcgt gggctacagg gccgcgctgc agggtcagga
     4741 tctgaacctg caggtcgggt actcgcaccc cgtctccatc gcgccccgcg aggggataca
     4801 gttcgaggtc ccgaacgcca cgaccatcat cgtgcgcggt atagacaaac agcgggtcgg
     4861 ccagaccgcc gcggagatca ggatggtccg cccgcccgag ccgtacaagg gcaaaggcat
     4921 ccgctacagc gacgagcagg tccggcgcaa ggtcggcaag gccggctagt cgggttcagg
     4981 aggagactat ggctactttg cagaagcgga tgcacaggga gaagcggcgc aagcgagtca
     5041 ggcgcaaggt cgtcggatcc gcggatcgcc cgcggctctc cgtgtaccgc tcgaacgtcc
     5101 acatctacgc ccagctgatc gacgacgacg cccaggtgac gcttgcggcg gccgactccc
     5161 gcgaggttgg ggccgccgag agccgcaagg aggccgcccg caaggtaggc gagctcatcg
     5221 cgagcagggc ctccgaggcc ggcatcgaga cggcggtctt cgacaggggc gggaacaaat
     5281 accacggccg catcgcggcg ctcgcagagg gcgcacgcga ggccgggctg aagctctaac
     5341 ctttttagga ggaaacgtct tgggtcgaca gagaaacgac aacaggcaag gaggccgcgg
     5401 acagggcggc ggggggcgtg gtggccgtca gcgcgacgcg tccgacctcc aggacagggt
     5461 cgtcgagatc cggcgcgtcg ccaaggtcgt caagggcggc cggcgcttca acttctcggc
     5521 cctcgtggtg gtcggcgacg gcaagggccg cgtcggcgct gggctcggga aggcaaacac
     5581 cgtccccgcg gccatccaga agggccagga caaggccaag cgcaacatgt tcaacgtgcc
     5641 gatgcgaaac acgaccattc cgcacgaggt cataggcaag ttcgagagct cttcggtgct
     5701 cctcaagccg gcctccgagg gtaccggcgt gatcgcgggc ggcggcgttc gggcggtcct
     5761 cgagctcgcc ggcgtgcgcg acgtgctgac caaagcgctc ggctcgacga cctcggtgaa
     5821 cctggtcaag gccacggtcg agggcttgca gcggctgcgc agcaaggccg agatcgagga
     5881 gatgcggggg gtgaagataa cgctatgagc cccctgaagg ttacgcaggt aaggagcgtt
     5941 ataggctcca aagcaggcca caagaagacg gtgcgggccc ttggtctgaa gcgcatcagg
     6001 gattcccggg tgcacgagga cacgccccag atcaggggca tggtccacaa ggtgcagcac
     6061 ctcgtcgagg ccgaggaggt ggactagcag tgaggctcaa cgatatttcc ccggcccctg
     6121 gttccacgag ggcgcgcaag cgcgtgggac gtggcacggg ctcggggcac ggaaaaacct
     6181 ctgggcgcgg ccacaagggc gccggtgccc gctcgggcgc ctcagcgttc gccggctacg
     6241 agggcggcca gatgccgctg cagcggcgcc tgccgcgcct caagggcgag gccaggggcc
     6301 gccacaccgt ggccaggccg accgtgtacg cgcccgtcaa cctgggcgaa ctcagggacg
     6361 tcgccggtga tacgataggc ccggacgagt tgcgggccgc cgggctcgtc aggaagaagg
     6421 ccgggctggt caagatcctc ggcaacggtg acattggacg cgccgttacg gtgcgggcgc
     6481 acgcgttctc caggtcggcg caggagaaga tagaggctgg cggcggaagc gtcgaggtat
     6541 tggagcacta gatatgttgg gatcgctggc caacgcctgg aagatacccg agctacggac
     6601 gaggctgatg ttcacgttcg ccatgctggc tgcgtaccgg ctgggggcct acatacccct
     6661 gcccggcgtc aggccagagg tactcggcgg cggtcttcag accagcaacc cggtcacggg
     6721 gctgttcggc acctttacgg gcggggcctt caacaacttc gcggtgttct cgctcgggat
     6781 aatgccctac ataacggccg cgatcgttat gcagctcatg acggtcgcca tcccccgcct
     6841 gcaggagctt gccaaagaag gcgagacggg gcagcagaag atcacgcagt acacccgcta
     6901 cttcacgctg gcgcttgccc tcgtgcagtc cgtggccatg gtcttcttct tccgctcggc
     6961 gcagggcggc ggcgcgctcg ccgggacgac acccgtagag atactcgtcg ccgtggtcac
     7021 cctcaccacg ggcgtcatgc tcaccatgtg gttcggcgag ctgataagcc agaggggcct
     7081 cggcaacggc atctcgctca tcataacggc ctcgatcctc tcccaggcgc cgcttgcctt
     7141 gcaggcgctc agggagaacg gcgatgtgct caccgtggta atactcgggt tgctcgccgt
     7201 ccttatcatc gcggccatcg ttttcgtgaa cgaggggcag cgcaggatcc ccatcaccta
     7261 cgccaagcgg caggtggggc gcaagatgag ccagggcggg accacctacc tcccgctcaa
     7321 ggtaaacatg gccggggtca tccccatcat cttcgcctcg tccctcctgc tcttccccgt
     7381 ggtgctaggg cagctggctt cgaggggcga tcccaattcc ttcctcggca ggctctccca
     7441 gttcttcgac ccgggcaacg cgccgtacct gatcctctac gcgctgttca tcatcatgtt
     7501 cacctacttc tacacggcgg tgcagttcaa ccccgtggag cacgcggaca acctcaagaa
     7561 gagcggcggc tacgtgcccg gctacaggcc ggggcagccg acagccatct acctgaacaa
     7621 cgtgctgacc aggatcacgc tcttcggcgc gctcttcctg gccgtggtgg ccgtgctgcc
     7681 gtacttcatc tccgacgccc tgggtctggg gaccagtatc ttcctcggtg gcacctcgat
     7741 gctgatcgtg gtaggcgtct ccctggacac ggtgcggcag ttggagagcc agttgatgat
     7801 gcgcaactac gaagggttcc tgaagagacg atgagggtta ttctgctagg gccccaggga
     7861 gcgggcaagg gaacgcaggc caagcgcctc gcggacaggg tcggcgccaa gcacatctcc
     7921 acgggggata tcgtgcgcgc cgagatcgaa tccggctccg agctgggcag gaaggttcag
     7981 gagtacaacg acaagggcga actggtgccg gacgagatca tcgtggagat ggccaagccc
     8041 cacctggagg acgcaggtgc ctggctgctc gacggcttcc cgcgcaacga ggcgcaggca
     8101 caggccctcg acacggcgct ggaggagatc ggtgaggacc tcgacgcggt tgtcgccctc
     8161 gaagcccccg acgaggatct cgtggagcgg ctctcgggaa ggcgtcagag ccaggccacg
     8221 ggcaagatct accacgtgga ctacgacccg cccccagagg acggcgacga ccccggcccg
     8281 ttcgtccagc gtgacgacga cgccgaggat gccatccgcc gccgcctgca gatctaccac
     8341 gaccagaccg agccgctcaa ggactactac gccgggcgcg acctcctcgt gacggtcgac
     8401 gccgcgcagg agataccgaa ggtgaccgaa gacgtgctcg aggcggtcgg gtggagggaa
     8461 gacgcttgat cgtccgcaag agcagaaagg aactcgaggc gatgcgcgag ggcggccgca
     8521 tcacggccgc ctgcctcgag atgttgaagg agaacgtcag gcccggcgtt accacgggcg
     8581 agctggaccg cctcgccgag gactttatcc gttccaacgg cgggaccccg gagttcaagg
     8641 gctaccccgg tagccccggc gcccctgact ttcccggctc aatctgcgcc tccccgaacg
     8701 ccatgatcgt gcacggcatc ccaggctcct accgcctgaa gagcggcgac ataatctcgc
     8761 tcgacgtggg tgtccgttat cagggcttcg tgaccgactc ggcgaggacc gtaccggtcg
     8821 gggacgtgcc cgacgacgtc gcggggctgc tccggatcac gcaggagtgt ctggaggctg
     8881 ccacggagca gatgcgctcg ggcaacaggc ttggcgacgt gggatacgcc atccagtcca
     8941 ccgccgagtc gaagggctac ggggtggtca gggaccttgt ctcccacggg gtagggcgcc
     9001 agatgcacga ggacccccag atccccaact acgggaaccc ggggacgggg ccgcgcttga
     9061 agccgggcat gaccttcgcc atagagccca tgatcacgct cggcgattac tccatccgta
     9121 tcagcgagtg ggacggctgg tccatctaca ctgcggatgg gtccctttcg gcccacttcg
     9181 agaacaccat agccgtcacc gagaacggcc cctgggtgct gacgcagaac ggggcaccgt
     9241 aagccgatag aaagcgatgt ttccggtggg agaggcgggc caggcgtgat aagatatttg
     9301 tttggcttca gccatcttgt aactatttga aaagaggagg tccttggcca aagaagacgt
     9361 catagaggtt gaaggcaccg taaccgaggc gctgcccaac acgcagttcc gggtcgagct
     9421 cgagaacggg cacaacgtgc tggcgcacat ctcgggcaag atgcggatga actacatccg
     9481 tatattgccg ggagacaggg tgaaggtgga gttgagcccc tacgacttga gccggggaag
     9541 gatcacctac aggttcagaa cttaaggaga tacagctgtg aaagtcaggg cgagcgtgaa
     9601 acctatttgc gagcggtgca aggtaatcag gcgccgcggg gtggtgcggg tcatctgcac
     9661 gaaccccagg cacaagcaga ggcagggata gcatggcgcg gatagcagga gtagaccttc
     9721 cgcgcgagaa gcgggtcgag gttggcctta cctacatcta cggcgtcggc cgttcgacgg
     9781 cgcgcaagat ctgccgcgac accgaggtcg acccggacac gaaggtacgc gacctggccg
     9841 agggtgaggt cggcaagatc cggacctaca tcgaccagaa cgtcgaggtc gagggcgacc
     9901 tgaggcggga gacgcagcag aacatcaggc ggctcataga tatagggacc tataggggca
     9961 tcaggcaccg caggggtctc cctgtgcgtg ggcagaggac caagaccaac gcccgccagc
    10021 gcaagggacc gaagccgtcg ataggcggga ggaagaagag gtagatgggc aggcagaggc
    10081 aacagcggca gcgccagcgc gggggggcca gcaggtcccg gcgtcgcgtc cgccgcaacg
    10141 taacgaccgg cgtggtccac ataaagagct ctttcaacaa caccatcgtc tccgtgagcg
    10201 accaggaggg caacgtcatc tcctgggagt ccgcgggttc gctagggttc aaggggagcc
    10261 gcaagagcac gccgttcgcg gcccagatga cggccgagag cgtcgccaac aaggcgatgg
    10321 accagggcgt gaggcgcgtg gacatcgagg tcaagggcca cggttccggt agggacatgg
    10381 ccgcgcgaac cttccagtcc atgggcatcg aggtgctctc cataaaggac gtgacgggcc
    10441 agccgcacaa cggttgcagg ccgcccaaga ggcgcagggg gtagctggtg gcacgttaca
    10501 cgggacctag aggaagaaga gaccgccgcg ccggcgtgat gctgtcttcg atgcggaaga
    10561 acccgctgga gaagaagcca tatccccctg gcgagcacgg caggggccgc caccgccaga
    10621 cggagttcgg catgcggctg atggagaagc agaaggcccg ctggtactac ggcgtctccg
    10681 agaaacagtt caggagggcc tacgacaggg cgaccaggat gcagggcgtc tctggcgaga
    10741 acctgctcag gctcatggag ctcaggatgg acaacgtggt ctttcgcctg ggcttcgcca
    10801 cgagcaggcc gcaggcgcgc cagctcgtcg tgcacgggca cttccagctc aacggccgca
    10861 agcacaacgt gccctcggcc attctcaagc cgggagatgt catcacggtc aaggagaaga
    10921 gcaggcagct tgagcccatc cagaacgccg tggagaacgt ggtggccgtc ccgccgtggc
    10981 tcgaggccga tcacgacaat ttcacgggca ggctactgca cacgccggcc cgcgacgaga
    11041 tagacacgcc cgtcgaagag cagctcatca tcgagttcta cagccggtaa gagcagaaag
    11101 atttctagca ggtgatattt tgacgatgtt ggacatagca ccaccccgtt tcagggtgga
    11161 agaagaagag accaggcgcg gcacctttgt tgccgagccg ctgccgcgtg ggctcggaca
    11221 caccctgggc aactccctgc ggcgcgtcat gctctcgggc ctgacgggcg cggcggtgac
    11281 caagattcgg atcgaaggcg tctcgcacga gttctcgacc atcgagggcg tgaaagagga
    11341 cgtcgtcgac atcatcctca acatcaaggg gctgaagttc aagctggagc gtgaggagcc
    11401 catagagctc acgatcgcca aggacggccc cggcgaggtc acggcggccg acatcgagct
    11461 gaaggccgac gttgagatcg tcgaccccga ggcctatata gcgagcgtct ccgacggcgg
    11521 gcacctcgac atgcgcctga cggtcgagag aggccagggc tacgccaggg ccgagcagaa
    11581 caagagcgac gcggacccca taggcgtcat agccgtcgac tcgctgttct cgccggtgca
    11641 gcgggtgaac tacacggtca ccgagacgcg tgccggggcg agggccgacc tcgactccct
    11701 gcggatggag gtcttcaccg acggccgcat cgagccgcag gaggcgcttc aggatgcctc
    11761 caggcagctc atggacttcc tcgggctctt cacggacggc taccgcgggg ccggggcacc
    11821 agaccccaac cagcgcggcg gaaggcccgt cataaccgac gagcgtcccg tggaggacct
    11881 ggagctcacg gttcgctcct acaactgcct caagcgcgag ggggtcgaca ccataggcca
    11941 gctcgccacc atgaccgaag aagagctcat gaacatccgc aacctcggga tgaagagcgt
    12001 cgacgagatc cgctccaagc tcctcgagta cgggtactca ctggagagcg agggcaatga
    12061 ggcacgctaa gcgcggcagg aagctgggcc gcgaggccca gcaccgcaac ctgatgctcg
    12121 gcaccatggc cggacaggtg ataacccacg gccgcataaa gacgacggcc ccgaaggcca
    12181 aggagctgcg cggcgtcgtc gacaagctca taaacaccgc caagaaggac gacctggcct
    12241 cccggcgcca ggcggtcaag gtcctgaagg acaagaccgt ggtgcgccac ctcttcgagg
    12301 atgtggcccc cgacctcgac gaccgcaact ctgggtacac gcgtatcctg aagctcggtc
    12361 ccaggctcgg cgacggcgcc gagaccgtct acctggagct ggtcaactac tcgacggagt
    12421 agccccagcg tgcagcactc cggcacaggg acccgccccg gtgggtcctt cgccgtttct
    12481 tgcgaaagct tgaccttcta ctacccaggg gccacggacc ccgcgctgtc tgaggtcggc
    12541 ttcgaattgc gtcccggcga gtacgtcggg gttgtcggcc cgaacggcgg gggcaaatcc
    12601 acgctcgtac gcctcctgaa cgggctcctg cgccctgact cgggtcgcgt gcgggtatcg
    12661 ggccacgacc cggccacgga acccttcgag gtccgcaagc ggcttggcat cctgttccag
    12721 aaccccgaga atggactcgt ggcacctttc gtcgaagacg acgtcgcctt cggcctcgag
    12781 aacctcggtg tcccgcggga gcagatgcgc gagagggtgg cgggggccat ccgggccgtc
    12841 gggctcgaag gctacgagcg ccgcgagccg cacacgctct ccggcggcga gaagcagcgg
    12901 gtcgcccttg ccggcctcct cgccgtggag ccggagatac tggtcctgga cgagccaacc
    12961 tcgatgctcg acgcggcggg tcgcagggag gttctggaaa gactcgagac acttcgttcc
    13021 cgaaagaccg tgctgcacgt tacccaccac ctggaggaac tgctccacgc ggaccgtgtc
    13081 ctcgtcctca acggggggga gctcgtggcc gacgagagcc cgcagcggct gctctccgac
    13141 cccggtctct tgcaggagaa caggctcatc ctgccggtcg tgcccaggtt ggcccaggcg
    13201 cttggactcc gggccgacct cggcacgccg gaggagctgg ccgaggccat cctgacgggg
    13261 acggggagcg ggacgagatc gggatgaggc tcgagctacg ggatgtgagc cacgtctacg
    13321 ccgccgggac gccgttcgag gtggaggcgt tgcgtggcgt ctcgctaacg gtcgagccgg
    13381 cagaggtgct cggcatcatt ggcggcaccg gttcgggcaa gagcacgctc gtgcggcaca
    13441 tgaacctgtt gctcgcaccg gccgacggtg aggtgctcgt cgacggcgtg gacgcgacca
    13501 cgttgaagaa gagcgagctg cggcgcaggg tcgggctcgt cttccagttc cccgaggcgg
    13561 cgctcttcgc cccaaccgtc ggggaggatg tggccttcgc tccgcgacac ctcgggctgg
    13621 acgaggaaga agtcggggaa cgtgtcaggg ggtcactcgg gttgctcggg gtcggggatc
    13681 ttgccgggcg ctcaccgttc gcgctctccg gcggggagag gcggcgcgtc gccatcgccg
    13741 gggtgctcgc gatgggcccg gaggtgctcg tgctcgacga gcccacggct ggactcgatc
    13801 ccgccaccag gagggaattg ctggccctca ttcgtcgcct gcgcgaggcg ggcgtctccg
    13861 tagttctcgt ctcccacgac ctcgacgagg tcgccgaggt cgccgaccgg gtgtgcgcac
    13921 tgcacgatgg tagcgtgcgc tctatcggta gtccggcaga ggtgttctac gcggagccag
    13981 accaggcgcc ggcgaccgta cgggttgcct ccctgcttcg ggcgtcgagg ccagatatcg
    14041 gggagcccgt gcgcttcgat gagacggtcg ccgccctcaa ggccctgccg ggggtgtgag
    14101 gtgacgagcc tcgggcagtt ctatccagtc gcctcaccgg tgcatgccct cgacccgagg
    14161 gccaagatag tggccaccgc cattctcgtc gccgggttgt tcctcgtcga ctcgatcgtg
    14221 ggcctgctgt tcgtcgcggc cgcgctcgtg gcgctcgtgg ccgcgagccg cattccggcg
    14281 cgagatttct tgcgtctcct caggcccgtg ctctttatcg tggcgctcac ggtcgtattt
    14341 cagctcctct tctcccggga gggggcgacg atcttcgagt tgggattcct ggaggttcat
    14401 accggcgggc tacggctcgc cttcttcctg gccttgcgga tactcctgct cgtggggata
    14461 gcgggcgtgc tcacggccac gacagccccc gtcgccctcg ccgacgggat agaagacctg
    14521 ctgtccccgt tgaagaaggt acggtttccc gcgcacgagg tcgccatgat gatgaccaga
    14581 gctctacgct tcatccccac gctgggggag gaggcggaga agatccgggg cgcccaggcc
    14641 gcccgcggag cggacttcac cgagggggga ccgctcagaa gggcccgctc gctcgtgccc
    14701 gttctcgtcc ccctgaccgt cggggccttc cgccgcgccg acgagttggc cgaggcgatg
    14761 gagagcaggg gctacaaggg gggcgaggga agaacccgct accgagagct a
//
LOCUS       DBNGVD010000078         4937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1109807, whole genome shotgun sequence.
ACCESSION   DBNGVD010000078 DBNGVD010000000
VERSION     DBNGVD010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1109807"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="aroC"
                     /locus_tag="AC16CM_02405"
     CDS             complement(<1..842)
                     /gene="aroC"
                     /locus_tag="AC16CM_02405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3138706.1"
                     /translation="MRFGFSTAGESHGPAEVALVHGVPAGLGLVARDVNRDLARRQKG
                     YGRGGRQKIERDEVEFLGGVRHGYTLGSPVAMLIRNNDYANWEHRMTPEPVEDPPDPI
                     TLPRPGHADLAGMQKYDFDDLRNVLERSSARETTARVAAGGVARKLLSEFGVEITSAV
                     YRIGAVAYPKENAALEAASADESEVRCPDPEVTERMKAEIDAARYARDALGGEFVVVA
                     RGLPPGLGSYVDWRDKLDARLAAAMMSINAIKGVEFGMGFELAARRSSEVQDEILREN
                     GRLAR"
     gene            complement(907..1770)
                     /locus_tag="AC16CM_02410"
     CDS             complement(907..1770)
                     /locus_tag="AC16CM_02410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3138707.1"
                     /translation="MTPSGAGPEGAGFLADLYALTGKVAVVTGATGVLGGAMARGLSR
                     AGAKVGVLGRRMEKAEEAASALTRGGTEALALPVDVLDRGQLEAAREAVLERWGRIDV
                     LLNAAGGNVPGAVVGEDDSFFEMSRGALGEVLDLNFMGTVLPTQVFGEAMIRGEGRAE
                     GSIVNVSSMAAQRPLTRVAGYSAAKAAVENLTRWLAAELVRKHGPGLRVNAVAPGFFL
                     GDQNRALLVGEDGSLTGRGSTIIEHTPAGRFGEPDDLVGAVVWLCGPGARFVNGVVIP
                     VDGGFSAFGGV"
     gene            complement(1877..2602)
                     /locus_tag="AC16CM_02415"
     CDS             complement(1877..2602)
                     /locus_tag="AC16CM_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138708.1"
                     /translation="MADRIRLLGRRIADSGSTLADALVRDNRVLPPVLAVLALFVIAW
                     VLAGVFMGDEPDQQTVAHRADLAQTELAQSDGGSGSDPAPGVENRDVDSYAAYRSKDP
                     FRELFSSESTTPEGTTPDSTTGGPRPGGGAGAGNTTRDTDSDGLPDRRERRTGTDPNN
                     RDTDADGTPDGSDDDANGDGRPDAGGTGAGTGSGTGSGTGSTPGSPGQPGRGDDRGGD
                     RGDDRGDSRGDGLLNSGGTLPLP"
     gene            complement(2592..3302)
                     /gene="pilO"
                     /locus_tag="AC16CM_02420"
     CDS             complement(2592..3302)
                     /gene="pilO"
                     /locus_tag="AC16CM_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIR3138709.1"
                     /translation="MTNRNILILGALGIVMLIILYYFLLLSPLLQNLDAQAQAKESKQ
                     AQLEQVQQQVNELEEVRRQSPDIERQLLELSKRIPAQPQVPTFVVQVQEIADASGATQ
                     LTVDPEPATSPEGGGDYRELPVTMQFTATYDEMQDFLLRTRNLARLVTVTNVTYCRPE
                     AETGQSENAPTCPSLPTGDVGEETTTLDPSVETVLAVEIAADIYYQPTDATSGEVPPT
                     VVPETTTPEQPEETTSGG"
     gene            complement(3299..4039)
                     /locus_tag="AC16CM_02425"
     CDS             complement(3299..4039)
                     /locus_tag="AC16CM_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3138710.1"
                     /translation="MRRINLLPPDERGRAAERFGGSVLGILLIAGALVVVVMVGVYLL
                     MLLRLNDLEDQVAQLDNDIAQQNARLAELSPYRDLQAQLDAKKPVADGIYSTRFAWDQ
                     FFQGLAFVVPESTALNALAAEASPVDLDAPPEQPLEPAGTVTFTGIALPRFENVADFV
                     VRMDNLQYLANAELDLASREVNSEDSPVTFEVASALVTIAGENGTEVKIEGASQDELA
                     AEERSSGTREVARVENVGAPGGPGGGNR"
     gene            complement(4036..>4937)
                     /gene="pilM"
                     /locus_tag="AC16CM_02430"
     CDS             complement(4036..>4937)
                     /gene="pilM"
                     /locus_tag="AC16CM_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3138711.1"
                     /translation="GVSNQKVVVRLLDFPHMRPEDLQGAISFEAQDHIPMPLDEAVLD
                     YVVLGPREERADLDRVLVVAAQREMISRYTTAVRAGGLKPEGVDVKALSLTRSTLPDP
                     FFGEDGAVLLLDVGSEITNLVVVDRGNPVLTRFVPVGLADFVAAVSELADLPDDEAER
                     QALDPRVNLGDVRVEEEPQQEEAVAEEDQKTEPFDPALAYDVRRGLEGAAQTLADEVQ
                     RSIEHHRSQEAARDVSRVLISGEAALIPGIDVYLGDLLGVPTGRGKPVDKLSANRSNI
                     EDEQLRAMEPVLAVAFGLAMEDE"
BASE COUNT          764 a         1729 c         1590 g          854 t
ORIGIN      
        1 cgtgccaggc ggccgttctc gcgcaggatc tcatcctgca cctcgctcga gcggcgggcc
       61 gcaagctcga agcccatccc gaactccacg cccttgatgg cgtttataga catcatggcc
      121 gccgctagcc tggcgtcgag cttgtcgcgc cagtccacgt aggagccaag cccgggcgga
      181 agcccccttg caacgaccac gaactcgcct cccagggcgt ctcgggcgta acgggcggcg
      241 tcgatctcgg ccttcatccg ctccgtaacc tcggggtccg ggcatctgac ctccgactcg
      301 tctgcacttg cggcctcgag cgcggcattc tccttcgggt aggctacagc cccgatcctg
      361 tagaccgcgc tcgtgatctc aaccccgaac tcggagagta gctttctggc gacgccgccg
      421 gccgcgaccc gcgcggtggt ttcgcgggcg ctggagcgct ccaggacgtt tcgcaggtcg
      481 tcgaagtcgt acttctgcat cccggccagg tcggcgtgcc cgggacgggg gagcgtgatc
      541 gggtccggcg ggtcttccac cggttctggc gtcatgcggt gctcccagtt ggcgtaatcg
      601 ttgttcctga tgagcatggc caccggggat ccgagcgtgt acccgtggcg tacccccccg
      661 aggaactcga cctcgtcacg ctcgatcttc tggcgtccgc cgcgaccata acctttctgc
      721 ctgcgcgcca ggtccctgtt cacgtcccgg gcaaccaggc ccagaccggc cgggaccccg
      781 tgcaccaggg cgacctcggc gggaccatgt gactcgcccg ccgtagaaaa accaaacctc
      841 aagaaacgcc tccgcatacg aacgacaact gcgggtagat tagcacgccc cggtcggggc
      901 gccgggctag accccgccaa acgcgctgaa gccgccgtcg accgggatca cgacgccgtt
      961 cacgaagcgg gcgcccgggc cacagagcca caccaccgca ccgacgaggt catccggctc
     1021 gccgaaccgc ccagcgggag tatgctcgat gatggtgctt ccccgtccgg tgagagagcc
     1081 gtcctcgcca acgagcagcg cccggttctg atcccccaga aagaacccag gcgcgacggc
     1141 gttgaccctg aggcctggcc cgtgcttgcg gaccagctcg gccgcgagcc agcgggtgag
     1201 gttctcaacc gccgccttcg ccgccgagta gccagccacc ctcgtcagcg gacgctgtgc
     1261 tgccatcgat gagacgttta ctatggaccc ctcggccctg ccttcccccc gaatcatggc
     1321 ctccccgaag acctgcgtcg gcaacacggt tcccataaag ttgaggtcca gcacctcccc
     1381 gagggcgccc cgcgacatct cgaaaaagct gtcgtcctct cccacgacgg ccccgggcac
     1441 gttgcccccg gcggcgttca gcagcacatc gatccttccc caacgctcca gaacggcctc
     1501 ccgggcagcc tctagctgac cccggtcgag cacgtcgacc gggagcgcca gcgcttccgt
     1561 gccaccccgc gtcagcgctg atgcggcctc ctcggccttc tccatgcgcc ggccgagcac
     1621 gccgaccttc gcgcctgccc gcgataatcc cctcgccatc gcgccgccga gcacgcccgt
     1681 tgcgcccgtc accacggcga ccttgcccgt gagcgcgtac aggtcagcca ggaaccccgc
     1741 accctctggc cccgcacccg acggcgtcat cacacgctac cgatcagcag gtgccccggc
     1801 cagtggttgg ccgcgcgccg cgctgcgtgg tgacgtgggg atatcgaggt gaacacccgc
     1861 aaggtcggga actttcctag ggaagaggca gcgttccgcc gctgttaagc aggccgtcac
     1921 cgcggctgtc gccacggtcg tcgccccgat cgccaccccg gtcgtcgcca cgcccgggtt
     1981 gcccggggct gcccggcgtc gaacccgtcc ccgacccggt accggacccg gtacccgcgc
     2041 ctgtaccgcc ggcatcgggt cgaccatctc cgttggcgtc atcatcggag ccgtcagggg
     2101 tgccgtcggc gtccgtatcc cggttgttgg ggtcggtgcc ggtcctccgt tccctgcggt
     2161 caggcaggcc gtcggagtcg gtatcccggg tggtgttacc ggcccctgct ccgccacccg
     2221 ggcgggggcc gccggtggtg gaatccggtg tggtcccttc aggggtcgtg ctctcggagg
     2281 agaacagttc gcggaagggg tccttgctgc ggtaggcggc gtaggagtca acgtcgcggt
     2341 tttcgacgcc gggcgcgggg tcgcttccgg acccgccgtc ggattgggcc agttcggtct
     2401 gggcgaggtc ggccctgtgg gccacggtct gctggtcggg ttcgtccccc ataaagaccc
     2461 cggcaaggac ccaggcgatc acgaacagag cgagcacggc gagcacagga ggcagcaccc
     2521 tgttatcccg cacgagggcg tcggcaaggg tcgaaccaga gtcggcgatg cgacgcccaa
     2581 gcaaccttat cctatccgcc actggtcgtc tcctcaggct gctcgggggt ggtggtctcc
     2641 gggaccacgg tcggggggac ctcacccgat gtggcgtcgg tcggctggta gtagatgtcc
     2701 gccgctatct ccacggcgag gacggtctcc acggacgggt cgagcgtcgt ggtctcctcc
     2761 cctacatctc cggttggaag cgacgggcag gtgggggcgt tctcggactg ccccgtctcc
     2821 gcctcgggcc ggcagtaggt gacgttggtt accgtcacaa gcctcgcgag gttacgtgtc
     2881 cgcaacaaaa agtcctgcat ttcatcgtag gtcgccgtga actgcattgt aaccgggagc
     2941 tccctgtagt cgccaccacc ctcgggcgag gttgcgggct cgggatcaac cgtaagctgg
     3001 gtcgctcccg aggcgtccgc tatctcctgt acctggacta cgaaggtggg gacctgtggt
     3061 tgcgcgggga tccgcttgct cagctccaga agttgccgct cgatgtcggg ggactgacgc
     3121 ctgacctcct cgagctcgtt gacctgttgc tggacctgct cgagctgggc ctgcttgctc
     3181 tccttcgcct gggcctgggc gtcgaggttc tgcagcaaag ggctcaagag gaggaagtag
     3241 tacaggatga tgagcatcac gatgccaagg gcgccgagga tgaggatgtt tctgttggtc
     3301 acctgtttcc gcctcccggt ccgccaggcg cgccgacgtt ttcgacgcgc gcgacctcgc
     3361 gggtgccgga ggacctctct tcggccgcga gctcatcctg agaggcgcct tcgatcttta
     3421 cctccgtgcc gttctccccg gcgatcgtga ccagcgccga ggcgacttcg aaggtcacgg
     3481 gcgagtcctc cgagtttacc tctcgcgacg ccaggtcgag ctccgcgttg gcgaggtact
     3541 gaaggttgtc catccgcacg acgaaatctg cgacgttctc gaaccggggc agcgcgatgc
     3601 cggtaaaggt taccgtgccc gccggttcga gtggctgttc gggtggcgca tcgagatcga
     3661 ccggtgaggc ctcggccgcc agggcgttca gcgccgtgct ctccggcacc acgaaggcga
     3721 ggccctggaa gaactgatcc caagcgaagc gggtgctgta gatgccgtct gccacgggct
     3781 tcttggcgtc cagttgcgcc tggaggtccc tgtacgggct caactccgcc aggcgggcgt
     3841 tctgctgcgc gatgtcgttg tccaactggg cgacctgatc ctcaaggtcg ttcaacctca
     3901 ggagcatcag caggtacaca ccgaccatca cgacgaccac gagggccccg gcgatgagca
     3961 ggatgccgag cacgctcccg ccgaacctct ctgcggccct gccccgctcg tcggggggaa
     4021 gcaggtttat acgcctcatt cgtcctccat ggcgaggccg aacgccaccg cgagcacggg
     4081 ctccatggcc ctgagctgct cgtcctcgat gttggaacgg ttggcggaga gcttgtccac
     4141 gggcttgcca cggcctgtgg gaacaccgag caggtccccg aggtaaacgt cgatgccggg
     4201 tatgagggcc gcctcaccag agatgagcac cctggagacg tcccgggcgg cctcctgcga
     4261 gcggtgatgc tcgatggagc gctgcacctc gtcggcgagc gtctgggccg cgccctccag
     4321 cccgcgcctt acatcatagg cgagcgccgg gtcgaaaggc tccgtcttct gatcctcctc
     4381 ggccaccgct tcctcctgct gcggctcctc ctcgacccgg acatctccaa ggttgaccct
     4441 cgggtcgagc gcctgcctct cggcctcgtc atcgggaagg tcggccagct ctgacacggc
     4501 ggccacaaag tccgccaggc caacgggcac gaagcgggtg agtaccgggt tgccccggtc
     4561 taccacgacg aggttggtga tctccgagcc aacgtctagc agaagcacag ccccgtcctc
     4621 cccgaagaag ggatcgggaa gggtggaccg ggtgagcgag agcgccttga cgtctacacc
     4681 ctctggcttc aaacccccag ccctgacagc cgtggtgtag cgcgagatca tctcccgctg
     4741 tgcggcgacc acgaggaccc tatcgaggtc ggcgcgctcc tccctgggcc cgagcaccac
     4801 gtagtcgagt acggcctcgt caagcggcat ggggatgtga tcctgggcct cgaagctgat
     4861 ggcgccctgc agatcctcgg gcctcatgtg cgggaaatct agaagcctta ccacgacttt
     4921 ctggttggag acgccga
//
LOCUS       DBNGVD010000079         2865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4753239, whole genome shotgun sequence.
ACCESSION   DBNGVD010000079 DBNGVD010000000
VERSION     DBNGVD010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4753239"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16CM_02435"
     CDS             complement(<1..670)
                     /locus_tag="AC16CM_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138712.1"
                     /translation="MSKPGRKPRTFSGSREWLFSGLAVGALGLMGGFIAVPILALVVW
                     TVNEQAWGAMASPVARDALLLSMKTTAATMTILIVLGTPAAYVLARGRFWGSRALNTL
                     VDLPIVLPPSAAGIALLLAFGRFGLAGQYLNFFGITLTFTTVAVVMAELFVAAPFYLR
                     QAATGFAEVDRAIEEASLVDGADRTATFFKVTVPLAFPALVAGAVTAWARALGEFGAT
                     IIFAG"
     gene            complement(684..1508)
                     /gene="modA"
                     /locus_tag="AC16CM_02440"
     CDS             complement(684..1508)
                     /gene="modA"
                     /locus_tag="AC16CM_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3138713.1"
                     /translation="MVYSIRSKNLVLACALAVLALVSCGGDGGGSSGDGERGGTLTVL
                     AASSLTDAFGELGDTFKEQNQGVEVKQTFGASSDLLAQIEQGAPADVFAPAAEEEMDT
                     ADEDGLISGTPRVFVENREVIMVPRDNPADINSLEDMARPDIKLVLAAKDVPAADYAV
                     EILGKANKEYGPGFERDVLSNVVSREADVRAAVNRVVVGDADATFGYASDYTPDIRNR
                     VKVVKIPPDLNIVATYPVAAMKDAEDSELARKWVDLVTSEEGQKVLERWNFEPAAR"
     gene            1744..1971
                     /locus_tag="AC16CM_02445"
     CDS             1744..1971
                     /locus_tag="AC16CM_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138714.1"
                     /translation="MTEGQREAPDRPEAERYYAQHASLDVEGAGSSQAALQAAINEGS
                     RHSWKLISVTKDPTNDADVILVWDTSGFFSG"
     gene            2078..2563
                     /locus_tag="AC16CM_02450"
     CDS             2078..2563
                     /locus_tag="AC16CM_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3138715.1"
                     /translation="MSEKTGLRENLRSEIEALVASDEPDETVLNEAVRRIHEARPLWD
                     WSGIYLLTGDTLVLGPRTAPADHSRIAIGDGVCGTAVAEDRNQIVEDVRELENYLACS
                     IHTRSELVVLIRDAGEIVGEFDIDSDTVGAFSTEDEALLEEMGTLISPRVASLARARS
                     G"
BASE COUNT          508 a          947 c          915 g          495 t
ORIGIN      
        1 ttcccgcgaa gattattgtt gccccgaact ctcctagcgc cctcgcccac gccgtcaccg
       61 ccccggcgac cagcgccggg aacgccagcg gtacggtgac cttgaagaac gtcgccgtgc
      121 ggtcggcccc gtccacgagc gaggcctcct cgatagcccg gtccacctcg gcaaagcccg
      181 ttgccgcctg cctcaggtaa aacggcgcgg ccacgaagag ctcggccatg accacggcaa
      241 ccgtcgtgaa ggtgagggtg atcccgaaga agttcaggta ctgtccggcg agcccgaacc
      301 ggccgaaggc caggagcagg gcgatgcccg ccgccgatgg cggaaggact atgggcagat
      361 ccacgagggt gttcaacgcc cggcttcccc agaaccggcc ccgcgcgaga acgtaggccg
      421 ccggcgtgcc caacacgatg aggatggtca tggtcgcggc cgtggttttc atgctcaaaa
      481 ggagggcgtc gcgcgccacg ggcgaggcca ttgcccccca ggcttgctcg ttcaccgtcc
      541 agaccacgag ggcgagtatc ggaacggcga tgaagccgcc catgagcccc agggcgccga
      601 ccgcaagccc ggagaagagc cattccctgc tccccgagaa ggtcctgggc tttcgcccgg
      661 gcttgctcac gagatgttcc ctcctacctg gccgcgggct cgaagttcca tctctccaga
      721 accttctgtc cctcttcgct cgtaacgagg tcaacccact tcctggctag ctcggagtcc
      781 tcggcgtctt tcatcgccgc tacgggatag gtggctacga tgttcagatc gggtgggatc
      841 tttacgacct tcaccctgtt ccttatgtcc ggggtgtagt cgctggcgta gccgaaggtg
      901 gcgtcggcgt caccgaccac gacccggttg accgctgccc gcacgtcggc ctccctggag
      961 accacgttgg agagcacgtc ccgctcgaag ccgggcccgt actccttgtt ggccttgccc
     1021 aggatctcca cggcatagtc ggcggccggc acgtccttcg cggcgagcac gagcttgatg
     1081 tccggcctgg ccatatcttc gaggctgttt atgtccgccg ggttgtccct gggtaccatg
     1141 atgacctcgc ggttctcgac gaacacccgg ggcgtgccag agatgaggcc gtcctcgtcg
     1201 gcggtatcca tctcctcctc ggcggcgggg gcgaacacgt cggcgggggc gccctgttct
     1261 atctgggcca gcaggtccga gctcgccccg aaggtctgct ttacctccac gccctggttc
     1321 tgctccttga aggtgtctcc gagctccccg aaagcgtcgg tcagggacga ggcggcgagt
     1381 acggttagcg tgccacctct ctccccatcc ccggagctcc ccccaccgtc cccaccgcac
     1441 gacactagcg ccagcaccgc cagggcacat gccagcacca ggttcttcga cctgatcgag
     1501 tacaccatcg actctccgtc catggaagct ttgccgcacc gggtaacgaa cgatccgtac
     1561 cgcaccgact catattaggg aacggtgggg acacaatctt cgtgcaaagt gcacagacat
     1621 gcagaccccc atctggcgtc ggcgcttgcc cgccaaaacc ccgaaagatg cacctggggc
     1681 cgatgaatgg catcatgtcc aagagggaaa gctcagcgag taaccgaggc aaggaggaag
     1741 agcatgaccg agggacagag ggaggccccg gaccgacccg aggccgagcg ctactacgca
     1801 cagcacgcct ccctggacgt ggagggggcc ggctcgtcgc aggcggcctt gcaggcggcc
     1861 atcaacgagg gttcacggca cagctggaag ctgatcagcg tgacaaagga cccaacaaac
     1921 gatgccgacg ttatcctggt gtgggacacg tcgggtttct tctccggata gaggggttat
     1981 tctgggggcg gttcgggaac cgccccgttt cgggacgtct cgcggggtcg gcgttatcat
     2041 tggtgggtca tgttccacgt acggaaggat tccatcgatg agcgagaaaa cgggactgcg
     2101 ggagaacctg agatccgaga tcgaggcgct cgtagcgtct gacgagccgg atgaaacggt
     2161 gctgaacgag gccgtgcggc gcatacacga ggcgcgtcct ctatgggact ggagcggcat
     2221 ctacctgctg accggggaca cgctcgtgct cgggccccgc acggcgccgg cggatcactc
     2281 gcgcatagcg atcggggacg gcgtctgcgg caccgccgtg gccgaggacc gcaaccagat
     2341 cgtggaagat gtgcgcgagc tggagaatta cctggcctgc tccatccaca cccgctccga
     2401 gctggtggtc cttattagag acgccgggga gatagtcggc gagttcgaca tcgattcgga
     2461 cacggtgggg gccttctcca cagaggacga ggctctgctt gaggagatgg gaaccctgat
     2521 ctccccgcgc gtcgcctcgc tggccagagc acgctcgggc tgacgcccac gcgtgcgcac
     2581 gctccggccc ttcgggctcc gcttgtcagc acactacggc ctgtcggcct tcgttcgtca
     2641 gcacgctcct tacggtcgct tgtcggcaac gcttcggctt cgcctcgctt tcggctagtc
     2701 gtctctctcc ctcacggcag ctccggcatg aggctttact gcgcggcgat accctctatg
     2761 cgaaaggtgc aggccgcagc gggatctggc cgaaagcgag ccctgaaagg gcgagacgtg
     2821 ctgacaagcg gaggcccgta gggccggagc gtgctctaca cgtga
//
LOCUS       DBNGVD010000080         8572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_563312, whole genome shotgun sequence.
ACCESSION   DBNGVD010000080 DBNGVD010000000
VERSION     DBNGVD010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_563312"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1186
                     /locus_tag="AC16CM_02455"
     CDS             293..1186
                     /locus_tag="AC16CM_02455"
                     /inference="COORDINATES: protein motif:HMM:NF020919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HIR3138716.1"
                     /translation="MKRLVTTFVAALVGSLALAGIAWADEPPVDQAPPLGWNWVCEPV
                     GDVVAVDPIASFGVEPTAHLHQPFCTSMESTLTLRELREQSNNTRWNVNPTLDTGAKN
                     LDWIPAPVFLEDPDTFVPVPHVNYYFRKAPNVPVSEVVPPPRGLKVIVGSPDRHDRVH
                     VGWRCGATLDSFSATQPDCVNQNLKLVVIFPPCSNGKPNSADHKSHLTWPGESGCPDT
                     HPIAIPQLRLEYALDNDGKRVAFFDHMGMRIAPHADFFNGTPTRVWEDVIERCLDNPK
                     SVGGCAGSGIEGTTTEEFGSV"
     gene            1642..2427
                     /locus_tag="AC16CM_02460"
     CDS             1642..2427
                     /locus_tag="AC16CM_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HIR3138717.1"
                     /translation="MLRTITATRYVTPLREGGSLPAIVEADDLGTYVLKFRGAGQGRK
                     ALVAELVAGEIGRRLGLLVPEVVLAYVDPELGRSEPDPEIQDLLKASSGLNLALDYLP
                     GSLGFDPLVEPPSSDLASRIVWFDALVTNVDRTPRNTNLLVWHRRLWLIDHGASLYFH
                     HNWPARNGTVLEAASRRPFAAARAHVLLPFASSIAEADAELCPLLTPEVLRDIVGMIP
                     TEWLADEPGFADAEEVRASYVEYLSTRLREPRDWVLALEEAAR"
     gene            2424..2813
                     /locus_tag="AC16CM_02465"
     CDS             2424..2813
                     /locus_tag="AC16CM_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HIR3138718.1"
                     /translation="MSLFEYALLRVVPRVDRGERINAGVVLYCQDARFLDARVHLDTE
                     RLRALDPNLDPEVIRAHLEVARIVCEGGPGAGAVGLLPPVQRFGWLVAPRSTVVQPSR
                     VHTGFTEDPGEAIEHLLRTMVHAPAEN"
     gene            2899..3951
                     /locus_tag="AC16CM_02470"
     CDS             2899..3951
                     /locus_tag="AC16CM_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3138719.1"
                     /translation="MTNTFIPGHGKRTTLRDVAREAGVSIKTVSRVVNREPLVNEATA
                     ARVAAVVEKLGYRPNELARSLKGQRSRTIGLVIDDVSNPFMAICAQAIEAVAREHGYA
                     LILCDSHADLREESTYIGLLMQRRVDGLLIVPARGHDSHLKVEQEAGLPVVAFDRPAE
                     GLSADTVLVENRQGTREATEHLIEHGHERIAFVGDVRHFYTARKRLEGYKEALEAARL
                     RPLHALDVHTIGQGEAAVKGFLDAPDPPTALFAANILTTLGALRAIEDLGLSIPEDIA
                     VIGFDDFELAHVLRPRFTLVRQPAAELGRRSAQLLFDRLDSKDDPGPRRIVLPTELVV
                     RESCGCPPPPTPDPSE"
     gene            4121..5548
                     /locus_tag="AC16CM_02475"
     CDS             4121..5548
                     /locus_tag="AC16CM_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIR3138720.1"
                     /translation="MDENVQGAEGGARVTGLLVATAVVSAIAGFLYGYDTGIISGALL
                     QIADEFKIGHGWQQVIAAGILVGAVIGALVGARVSERFGRKRTILVISAVFVVGTIAC
                     SLAPTALTLSLSRVLLGMAVGGATQTVPMFVAELSPPQVRGRLVLAFQVAIGVGILTA
                     TIVGATQLFDWRWMVGIASVPAALLLVLMLRAPESPRWLVKVDRVDDARSNLRRVRGE
                     GDVDGELDEIVAVEEEERRAPVSGWKGLSQPWVRPAVIMGCGMALFTQLSGIEMIVYY
                     TPTILTNVGFAEASALYVSVSLAVTYVIMNCLGLAIVDRVGRRRLSLIMTPGAAIALA
                     VLGAVFVFGADSASTAPFIVACLIAFMLFTAGGLQVMGWLTGSEVYPLGVRGAGTSAQ
                     AATLWGSNVVITLTLLTTIETIGVGQTMWMYAGFNVLAFLFVLRYFPELAGRSLEDIE
                     TSLREDTFRPGGVPIRDEAEEKVAT"
     gene            5638..6672
                     /locus_tag="AC16CM_02480"
     CDS             5638..6672
                     /locus_tag="AC16CM_02480"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3138721.1"
                     /translation="MDRVTPETGNGTGENRSVVWDGSEGVEVESREVPAPGPGELRVR
                     VEASGLCGTDLHIASGEYPFAKSNVTLGHEFSGTVVGLGEGVDAFSEGDLIVVDPNIP
                     CRVCRYCHDSRPHLCENPQAIGITRDGGLSEFALLPVSQAYRVPDGLPPEAAALTEPL
                     ACALHAVDLGGLRPGERALVLGAGPIGMLCSMLLVAAGASEVLVSEPNRDRRERIRRI
                     GAEPLDPESVSEQMADRVFECVGRPETMAAAVEAANPGGTVMWVGVAPPDAEVPVKPY
                     DLFSRELTIRGSYTNPYVMHRSLALLASGRLDWETVVTHRFALEDFGEAWDAHREAAG
                     LKISVQPVAN"
     gene            complement(6908..>8572)
                     /locus_tag="AC16CM_02485"
     CDS             complement(6908..>8572)
                     /locus_tag="AC16CM_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3138722.1"
                     /translation="VGAVDTAGRAVISAGIVVVIALLGLLTIGIPFVSALGVAAAMVV
                     ALAVLVAVSLLPALLGLVGHRVDRWRIPGLRATGAVATRDERERTASYRLSRLIQRHP
                     WPYAIGAALLLLALTAPVLDMRLGFSDDGNKPTSFDSRKAYDLLAEGFGPGFNGPLVV
                     AIEQDGGLDRATLDRLSKRLDDAKNVDSVTPPQPNEAGNAAVLTVYPATSPQSAATED
                     LVDRMRDDVVPDALDGTDAVAYVGGATASFVDIGERIAGRMPIFFAFVVGLSFLLLMA
                     VFRSVVVPVKAAIMNLLSVGAAYGVLVPIFQWGWGESLLGIKPGPIEAFLPMMLFAIL
                     FGLSMDYEVFLVSRIRESYLRSGDPRGSVADGLAITARVITAAAAIMVCVFLSFVLGD
                     DRIIKEFGLGLAAAIFVDATVIRLVLVPSTMTLLGRWNWSFPRWLDAIVPRLNIEGDL
                     STPPPVEDNAAASRLILAGLSMEYLARRLEEADGDYSNLTRAAAALLPEESKNGNSTH
                     QNARLVTRNVLRPLALRAMLDGVRAAGTNGSEEGSPEPPKVRQSRG"
BASE COUNT         1518 a         2750 c         2840 g         1464 t
ORIGIN      
        1 tgtacggagc cttttctgtg ccattccgcc gtgctcgtga cgatgtggct gtgtatcgtg
       61 gatcagaatt ccctaccacc cgatcgtgcc gacttcggat tcacggcgtt ctgaagttgg
      121 ccaggcgttc cgaacgtgat agcccgttcg gttgcgaaac atggacttag agtggactaa
      181 gacttagcca cacggtcgat tcgtatgctt cgtgccgccc caatacttga agttgtcatg
      241 tgggcgagac ggagacgcac ttccttcctc cgtccctcta gggaggaaag acatgaagag
      301 gctagtgacg acgttcgtag ctgcgctggt aggttccctg gcgcttgccg gcatcgcctg
      361 ggccgacgag ccgccggtcg accaagcgcc gcctcttggc tggaattggg tatgcgagcc
      421 cgtcggagac gtggtagccg tggacccgat agcaagcttc ggcgtcgagc ctaccgcgca
      481 cctgcatcag ccgttttgta cctcgatgga gtccacgctc acgctccgcg agctcaggga
      541 acaatcgaac aacaccaggt ggaacgtgaa tccgactcta gacaccggag ccaagaacct
      601 cgactggata ccagctcccg tattcctgga ggacccagac accttcgtac ccgtgcccca
      661 cgttaattac tacttccgca aggcccccaa cgtgccagta tccgaggtgg tgccgccgcc
      721 gcgcggcttg aaggtaatag tgggtagccc ggaccgacac gatcgggttc acgtcggctg
      781 gcgatgtggc gccacgctgg acagcttctc tgctacgcag cctgattgcg tcaaccagaa
      841 cctcaagctc gttgtcatct tcccaccgtg ctctaacggg aaaccaaaca gcgccgatca
      901 caaaagccac ctcacatggc caggagagag tggctgtcct gatactcacc cgatagcgat
      961 cccccaattg cgcctggagt atgcactcga taacgacggc aagcgggtag ccttcttcga
     1021 ccacatgggg atgcgtatcg cgccacacgc cgacttcttc aacgggacgc cgaccagagt
     1081 gtgggaggac gtcatcgaga ggtgcctcga caaccccaag agcgttggag ggtgcgccgg
     1141 ttccgggata gaaggcacga cgaccgagga gttcggaagc gtctgacgag agaaaattgt
     1201 gcagtgcctc agaggaggct gttcagaaga tggtgagcgc atacgggagc aaccgccgtg
     1261 cctccaccgt gctacgttag tgggtccgga tggcggagcg aagaatgcta accattagcg
     1321 atccgcagct acaaagaggc agcaacggat caagaggtca ggggctaata ttagcccctg
     1381 acctcttcta ctatcaccat tattcaccag agtgcgtgga aggtaaattc tcgaaagtcg
     1441 gaatgcagga tcctgcataa tttgccgccg cgtaggctgc ggagccagct tttcgccccg
     1501 gtctagccgt gctccggaca cgagatgttg tggtgccgga tcaatatgca aaagcgtgca
     1561 tgatttgtga cggggttccg gactagtcgg tagaatgagg gtgccgggtc tctccgcaac
     1621 gtcgaagtcg aggaacgcag cttgctcagg acgatcaccg cgacgaggta cgtaacaccc
     1681 ttgcgtgagg gtggttcgct acctgcgatc gtggaggccg acgatctggg tacgtacgtg
     1741 ctcaagttcc ggggtgccgg gcaggggcgc aaggctctgg ttgcggagct ggtcgcgggg
     1801 gagataggac gccgcctcgg cctcctggtg cccgaggtcg tactcgccta cgtcgacccc
     1861 gagctcggcc gctccgaacc agaccccgag atacaggatc tcctgaaggc gagttccggg
     1921 ctgaacctcg cgctcgacta cctgccgggt tctctgggct tcgacccgct ggtggagcca
     1981 ccgagctccg acctagcctc gcgcatcgtg tggttcgacg ccctggtgac caacgtggac
     2041 cgtaccccgc gcaacaccaa cctcctggtg tggcaccgtc gcctctggct catcgaccac
     2101 ggggcctcgc tgtacttcca ccacaactgg cccgccagga acggtacggt cctcgaagcc
     2161 gcgagcaggc gccccttcgc ggcggcgagg gcccacgtgc tactaccctt cgcaagctcc
     2221 atcgcagaag ccgacgccga gctgtgcccg ctactgaccc ccgaggtgtt gcgagacata
     2281 gtgggcatga tccccacgga gtggctcgca gatgagcctg gtttcgccga cgccgaggag
     2341 gtgcgcgcct cctacgtgga atacctgagc acccgccttc gggaacccag ggactgggtt
     2401 ctggcgctgg aggaggccgc gagatgagcc tcttcgagta cgcgcttctg cgcgtcgtgc
     2461 cgcgcgtcga tcgcggggag cgcataaacg ccggggtcgt gctctactgc caggacgcga
     2521 ggttcctcga cgcgcgcgtt cacctcgaca ccgaacgctt acgggcgctc gaccccaacc
     2581 tggaccctga ggtcatccgg gcacatctgg aggtcgcgag gatcgtttgc gagggtgggc
     2641 caggggccgg cgccgtcggc ttgctgccgc cggtccagcg gttcggatgg ctcgtcgcac
     2701 cccgcagcac agtagtacag ccctcacggg tacacacggg cttcaccgag gatccaggcg
     2761 aggccataga gcacctgctg cgaaccatgg tccacgcgcc cgcagagaac tagcgacctc
     2821 ggatccaccc acgccccgga cgtccggggc gtatgccgtt gctctccatg tgatccggtg
     2881 gtagcgtggg ctgagaccat gacaaacacg ttcataccgg gacacggcaa gagaaccacc
     2941 ctgcgcgacg tcgcccgcga ggcgggggtc tcgatcaaga ccgtctcccg ggtggtgaac
     3001 agggaacccc tcgtcaacga ggcgacggcg gcaagggtgg ccgccgtggt ggagaagctc
     3061 ggctaccggc cgaacgagct ggcacgctcc ctgaaaggac aacgttcacg cacgataggc
     3121 ctggtgatag acgacgtctc gaaccctttc atggccatct gcgcccaggc catcgaggca
     3181 gtggcccgcg agcacggcta tgccctgatc ctctgcgact cacacgcgga cctgagggag
     3241 gagagcacct atatcgggct cctcatgcaa cggcgggtcg acgggctcct gatagtgccg
     3301 gccagggggc acgacagcca cctaaaggtt gagcaggaag ccgggctccc cgtcgtcgcc
     3361 ttcgaccggc ccgcagaggg cctgagcgcc gacacggtgc tcgtggagaa ccgccagggc
     3421 acgcgggagg cgacggaaca cctgatcgag cacggccacg agcgcatagc cttcgtcggg
     3481 gacgtccgcc acttctatac cgcccgcaaa cgcctcgaag gctacaagga ggcgctggaa
     3541 gccgcgcgcc ttcgccccct ccacgccctc gatgtccaca ccatcgggca gggcgaagcg
     3601 gcggtgaaag gcttcctgga cgcaccagac ccgccaacgg ccctgttcgc cgccaacatc
     3661 ctgaccaccc tgggcgcgct cagggccatc gaagacctgg ggctgagcat ccccgaggac
     3721 atcgccgtga tcggcttcga cgacttcgaa ctcgcccacg tcctgaggcc ccgatttacc
     3781 ctggtcaggc agccggccgc agagctgggc cgccgctccg cccaactgct attcgatcgc
     3841 ctcgatagca aggacgaccc ggggcccaga cgcatcgtcc tgcccacaga gctggtcgtg
     3901 cgagagtcct gcggttgtcc cccaccgcct acccccgacc cgtccgagta aacaccccga
     3961 cccgatacgc ccagcacgcg ccagacgtct tgacaacgtt atcagcagcc gttagatttc
     4021 ccgacaacgt gctgacaacc ttgtcagcaa gatgatcaca gagggggttt cgtggggcac
     4081 tcttgggcct tgcgttgccg ggatcaggag aggagcaggg gtggatgaga acgtgcaagg
     4141 ggcggagggt ggggcgcggg taaccggcct tctggtagcg acggctgtgg tatcggcgat
     4201 cgccggtttc ctctacggtt acgacaccgg gatcatctct ggggcgctcc tacagatcgc
     4261 cgacgagttc aagatcggcc acggctggca acaggtcatc gcggcgggga ttctggtcgg
     4321 cgcggtcatc ggcgcgctcg tcggcgcgcg cgtctcggag cggttcgggc gcaagcgcac
     4381 cattctggtc atctcggcgg tgttcgtggt cggaaccatc gcctgcagcc tcgcgccgac
     4441 ggcgctcacg ctctcgctct cgagggtgct gctcggcatg gcggtcgggg gggcgacgca
     4501 gacggtgccc atgttcgtcg ccgagctttc gccgccgcag gtccgcgggc ggctcgtgct
     4561 ggccttccag gtggccatcg gggtggggat actgaccgcg acgatcgtcg gggccaccca
     4621 gctctttgac tggcggtgga tggtcggcat cgcgtccgtg cccgcggcat tgctactcgt
     4681 cttgatgcta cgggcaccgg agagcccgcg ctggctggtc aaggtagaca gggtcgacga
     4741 tgcgaggagc aacctgaggc gcgtgcgggg cgagggggac gtcgatggcg agctcgacga
     4801 gatcgtcgct gtcgaggaag aggagcggcg ggcgcccgtc tccggctgga agggtttgag
     4861 ccagccgtgg gtacggcccg ccgtcatcat gggttgcggg atggcgctct ttacccagct
     4921 aagcggcatc gagatgatcg tctactatac gccgacgatc ttgaccaacg tcgggttcgc
     4981 ggaggcgagc gcgctgtacg tcagcgtctc gctcgcggtg acctacgtca tcatgaactg
     5041 cctcggcctc gccatcgtgg accgcgtggg ccggcgcagg ctctccctga taatgacccc
     5101 gggcgcggcg atcgcccttg cggtgctcgg ggctgtgttc gtgttcggtg ccgacagcgc
     5161 ctccacggcc ccgttcatag tcgcgtgcct gatcgcgttc atgctcttta ccgcgggagg
     5221 gttgcaggtc atgggctggc tgacgggctc ggaggtatac ccgcttggcg taaggggcgc
     5281 tgggaccagc gcccaggcgg cgacgctctg ggggtccaac gtggtcatca ccctcacgct
     5341 gctaacgacg atcgagacga taggggtcgg gcagacgatg tggatgtacg ccgggttcaa
     5401 cgtgcttgct ttcctcttcg tcttgcggta cttcccggag ctcgcggggc gcagcctcga
     5461 ggacatcgaa acctcgctca gggaggacac gttccgcccc ggcggcgtcc ccatcaggga
     5521 cgaagcagaa gagaaggtgg ctacctgatc tctgccggga ggtccggtac cctttattct
     5581 tcgggagcgg tgagtagaac gtggggcttc ggccgcgcac aaagttagga gaggtgcatg
     5641 gatagagtga cacctgagac cggaaacggc acgggggaga acaggagcgt cgtctgggac
     5701 ggtagcgagg gcgtcgaggt agagtccagg gaggtccccg ccccagggcc gggagagttg
     5761 agggtgaggg tcgaggcttc cggactgtgc gggactgacc ttcacatcgc ctcgggagag
     5821 taccccttcg ccaagtccaa cgttaccctg ggccacgagt tctctggcac cgtggtgggg
     5881 ttgggagagg gcgtcgacgc cttctccgag ggcgacttga tcgtggtcga cccgaacata
     5941 ccgtgccggg tgtgccgcta ctgccacgac tcgcgcccgc acctttgcga gaacccccag
     6001 gccatcggta ttacccgcga cggcggcctc tccgagttcg ccttgctccc ggtatcgcaa
     6061 gcctaccgcg ttcccgacgg gcttcctcca gaggcggccg ccctgacaga gccgcttgcc
     6121 tgcgcgctgc acgccgtgga tctggggggc ttgcggcccg gagagcgggc gctggtcctc
     6181 ggggccggac ccatcggcat gctgtgctcc atgctgctcg tggcggccgg cgcctctgag
     6241 gtcctggtca gcgaaccaaa cagggacagg cgcgagcgaa tccgccgtat cggcgccgaa
     6301 cccctcgacc cggagtcggt ctcggaacag atggcggaca gggtgttcga gtgcgtcgga
     6361 aggcccgaga cgatggcggc cgccgtcgaa gccgcgaacc ccggcggcac ggtcatgtgg
     6421 gtcggggtgg ccccgccgga cgccgaggtc cccgtcaagc cctacgacct gttcagccgg
     6481 gagctaacaa tccgtgggtc ctataccaac ccatacgtca tgcaccgctc cctggccctg
     6541 cttgcctcgg gccggctgga ctgggagacg gtcgtaaccc accgctttgc cctcgaagac
     6601 ttcggggagg cgtgggacgc ccatcgcgaa gcggccgggc tcaagatctc cgttcagccg
     6661 gtggcgaact aggcgctgac gccgcattcg cagtctacgg gaaccgacca ggggcgcctc
     6721 tgttctacca ggggtcgggg cttcctcttc cccatcggtc aggacatccg tctctgcatc
     6781 tggcatcccc gtaaagcgtg ccgcccatct cacctcggag aaggtatcgg gcatgacgac
     6841 ccaccaggac ggtggccggc agcccgacct actttcccct ctcgcgttgg acgtgacgcc
     6901 ttgcgggcta tcctcgggac tgccggacct tcggcggttc ggggctgccc tcctcggagc
     6961 cgttggtacc ggcggcccgg acaccgtcca gcatggcccg tagtgccagc gggcgcagga
     7021 cgtttctcgt gaccagccgg gcgttctggt gcgtgctgtt tccgttcttc gactcctccg
     7081 gcaagagagc cgccgccgca cgggtcaggt tcgagtagtc cccgtcggcc tcctccaacc
     7141 tgcgggccag gtactccatc gagagcccgg cgaggatcag cctggaggcc gcagcattgt
     7201 cttcgaccgg gggcggcgtg gagaggtcac cctcaatgtt aagcctcggt actatggcat
     7261 cgagccaccg gggaaagctc cagttccacc ggccgagcag cgtcatggtg gaggggacga
     7321 ggacgagccg gatcacggtc gcgtccacga agattgccgc ggctaggccg aggccaaact
     7381 ccttgatgat cctgtcgtcc ccgagcacga aagagagaaa cacgcagacc atgatcgccg
     7441 ccgccgccgt gatgacgcgg gcggtgatcg ccagcccgtc ggccaccgaa cccctcgggt
     7501 cgccacttcg caggtagctc tcgcggatgc ggctaaccag gaagacctcg tagtccatgg
     7561 agaggccgaa gaggatggcg aagagcatca tcggtaggaa ggcctctatg gggcctggct
     7621 ttataccgag gaggctctct ccccagcccc actggaagat gggtacgagg acgccgtagg
     7681 cggctcccac ggagagaagg ttcatgatag cggccttcac gggcacgacc accgagcgaa
     7741 agactgccat gagaagcagg aagctcaggc ccacgacgaa ggcgaagaag atgggcatgc
     7801 ggccggctat ccgctcgccg atatccacga acgatgccgt tgcgccgccc acgtaggcta
     7861 cggcatccgt gccatcgagc gcgtcaggca ccacgtcgtc acgcatcctg tcgacgaggt
     7921 cctccgtggc cgcgctctgc ggggaggttg cggggtagac ggtcaggacg gcagcgttgc
     7981 ccgcctcgtt gggttgcggg ggcgtcacgc tgtcgacgtt tttggcatcg tccagacgct
     8041 tcgagagccg gtccagcgtc gcccggtcga gcccaccgtc ctgctcgata gcgaccacga
     8101 gcggcccgtt gaagccgggg ccgaacccct cggctagcag gtcgtaggcc tttctcgaat
     8161 cgaaggaggt cggcttgttg ccgtcgtcgg agaagccgag gcgcatgtcg agtacggggg
     8221 cggtcagcgc cagcaagagc agcgccgccc ctatggcgta gggccaaggg tggcgctgga
     8281 tgaggcggct caagcgatag ctcgccgttc gctccctttc gtcccgggtt gccacggcgc
     8341 ccgtggccct gaggcctggt atgcgccagc ggtccacacg gtgccctacg aggccaagta
     8401 gcgcaggcag gagcgagacc gccacgagca cagcgagggc gaccaccatg gccgccgcga
     8461 ccccgagggc cgagacgaac ggtatgccta tggtcaggag cccgaggagt gctatgacca
     8521 ctacgatgcc ggcagagatc accgcacgtc ccgccgtgtc tacggccccc ac
//
LOCUS       DBNGVD010000081         2502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_381986, whole genome shotgun sequence.
ACCESSION   DBNGVD010000081 DBNGVD010000000
VERSION     DBNGVD010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_381986"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="AC16CM_02490"
     CDS             <1..964
                     /locus_tag="AC16CM_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138723.1"
                     /translation="NLSGWRVVKTRDGREEAAPREDKEGLFERLARSVRSGGAGVAAA
                     SGTAPGSRNVPQTRYEDVGGMRETIDMIREAVELPITHPEIFRRLHIRPHKGILFFGP
                     PGTGKTLLARAVANESGAHFIAVSGPEILNKFWGQSEARLRSIFNEARAKAPAVILFD
                     EIDSFASSRDMMSESFEATLVSQLLSLMDGLNNLGRVCVIATTNRPSALDPALRRPGR
                     FDHEIEVGLPDAEARLHILGIHTREMPTDPDLNLKQIASLTGSYSGADLEALCREAAL
                     VCMRRTINLGDFEKRIASHQLSALSVATYDFRTAMKRVGPSIRR"
     gene            1190..1327
                     /locus_tag="AC16CM_02495"
     CDS             1190..1327
                     /locus_tag="AC16CM_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138724.1"
                     /translation="MVRLHEMFLDVFYGNTVGSGHLCQLEVPDQIGAMVDRFLAAALP
                     R"
     gene            1405..1680
                     /locus_tag="AC16CM_02500"
     CDS             1405..1680
                     /locus_tag="AC16CM_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138725.1"
                     /translation="MVYIHEIMAGYLQWAAYSVTLLAGALTDGLLALAMRRGFGRRGF
                     GRGLGPFRLIRLSRILFVLLFTTILGPIVLVAILVFAGYWFFASRRG"
     gene            1818..>2502
                     /locus_tag="AC16CM_02505"
     CDS             1818..>2502
                     /locus_tag="AC16CM_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3138726.1"
                     /translation="MLQTIINGIVTVGGVCLLALIGFELVHRLVPAVSRQRSNDVAGF
                     IYAALGVIYAVLLALVVIAVWEEYQSAGETVEQEANATAEIFWLGNRLPEPEGAHVQE
                     LAQSYAEEVVHKEWPLMRQGESPPLEQEGDAPTGWSIIDDIRASLQDYDPQSMAEAQV
                     YSKGLDQVEALNDSRRMRLVAAEEGVPGVLWSVLIFGGIAAVSFTYLFGLESTWAHRL
                     MVVTLAAVIG"
BASE COUNT          440 a          809 c          822 g          431 t
ORIGIN      
        1 gaacctctcc ggctggcggg tggtcaagac acgcgatggg cgtgaggagg cggccccgcg
       61 tgaagataaa gagggcctct tcgagcgcct ggcgaggagc gtgcggagtg gtggggccgg
      121 ggtcgcggcg gcgagtggta ccgcgccggg tagccgcaac gtgccgcaga cccgctacga
      181 ggacgtgggc gggatgcgcg agaccataga catgatacgc gaggcggtcg agctgcccat
      241 aacgcacccc gagatcttcc gccggctgca catcaggccg cacaagggca ttctcttctt
      301 cgggccgccc gggacgggca agacgcttct ggcgcgggcc gttgccaacg agagcggggc
      361 ccacttcata gccgtctcgg ggccggagat actcaacaag ttctggggcc agagcgaggc
      421 caggctgcgc tccatcttca acgaggcgag ggccaaggcg ccggccgtca tcctcttcga
      481 cgagatagac tccttcgcct cctcgcgcga catgatgagc gagagcttcg aggcaaccct
      541 cgtctcccag ctcctctccc tcatggacgg gctgaacaac ctcgggcgcg tctgcgttat
      601 cgccaccacc aaccggccct cggcgctcga ccccgcgcta aggcggccgg gccgcttcga
      661 ccatgagatc gaggtcggcc tccccgacgc cgaagcgcgg ctgcacatac tgggcatcca
      721 cacccgcgag atgcccaccg accccgacct caacctgaag cagatagcat cccttaccgg
      781 aagctactcg ggcgccgacc tggaggccct gtgccgggag gcggcccttg tgtgcatgcg
      841 ccgaaccatc aacctgggcg acttcgagaa gcgcatagcc tcccaccagc tctcggccct
      901 ctccgtcgcc acctacgatt tccgtaccgc catgaagcgc gtgggtccga gcattaggcg
      961 ttgacctgga gtaaataggg agtgccggct cgtcgaggga gagtacgaag cgtctgcggc
     1021 gcgacgggat gtatctctac taagaggagg tgcttcgctg gtacgattcg ggctcgctct
     1081 tcatccccac cgacgacccc gaccgcaagc agaacatcct ctccgccatg acctcgaccc
     1141 cgcagcacgt gagtacattg ccgccgacga ggcgcaacca cgctcggaca tggtccgttt
     1201 gcacgagatg ttcctcgacg tcttctacgg caataccgtt ggctcgggac acttatgcca
     1261 gctggaggtg ccggatcaga tcggggccat ggtcgaccgc ttcctcgccg ccgcgctccc
     1321 gcgttgagca cgtaggcttc aggcccccgc gctctggtat aacgccagaa gacgcggcag
     1381 cgaacgaaag gagtatcagg ctctatggtc tacatccacg agatcatggc ggggtatctc
     1441 cagtgggccg cgtactccgt cacgctgctc gccggcgccc tgaccgacgg gctcctggcc
     1501 ctcgccatga ggcggggctt cggccggcgg ggcttcggga gggggctcgg gccgttcagg
     1561 ctgatccggc tgagcaggat ccttttcgtg ctgctcttca ccaccatcct cgggccaata
     1621 gtgctcgtag ccatcctcgt cttcgccgga tactggttct tcgccagccg ccggggttag
     1681 accccgggcg cgtgatgcgg ccgttgatca ggagctgaag gctgaaagcc aaccgctgat
     1741 ggccaaaaac attggcgcgg cgtgcgcagt agaggctaga atgctgctgg ccttattcac
     1801 gaggggagat ttccgacgtg ctgcaaacca ttatcaacgg cattgttacg gttgggggag
     1861 tctgcctgct cgcgctcatt gggttcgagc tggtgcaccg cctcgtgccg gccgtctcgc
     1921 gccagcgctc gaacgacgtg gccggcttca tctacgcggc cctcggcgtg atctacgccg
     1981 tgctactggc gctcgtggtg atcgccgtgt gggaggagta ccagtcagcc ggtgagacgg
     2041 tcgagcagga ggccaacgcc acggcagaga tcttctggct gggcaatcgg cttcccgagc
     2101 ccgagggcgc ccacgtacag gagctcgccc agtcctacgc cgaagaggta gtccacaagg
     2161 agtggccgct gatgaggcag ggcgaaagtc ctcccctcga acaggaggga gatgccccga
     2221 ccggctggtc catcatcgac gacatcaggg cgagcctcca ggattacgac ccgcaatcga
     2281 tggccgaggc acaagtctac tccaaggggc tcgaccaggt cgaggcgctg aacgattcca
     2341 ggaggatgcg ccttgtcgcg gcagaggagg gggtgcccgg agtgctctgg tcggtcctga
     2401 tcttcggggg cattgccgcg gttagcttta cctacctctt cggcctggag agcacgtggg
     2461 cgcacaggct catggtggtg accctggcgg ctgtgatagg gc
//
LOCUS       DBNGVD010000082         5945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3296512, whole genome shotgun sequence.
ACCESSION   DBNGVD010000082 DBNGVD010000000
VERSION     DBNGVD010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3296512"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="AC16CM_02510"
     CDS             complement(<1..1330)
                     /locus_tag="AC16CM_02510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3138727.1"
                     /translation="MKWIGWIREQPWRITLAAVGGLLIAIFIAGFVGLLSNLRVEGVT
                     DEARYDLALEDEGDDMRAAVLDVRHYQRNITFSGPSEVQIDNFEASYDRLGEEIEELE
                     NTGVRDQDVTQPAQIRKMAERYYRDFRPAIELYETDRDAFERASARGLDRLDELAQIG
                     ESLDDLGETLSQKSLSELDAATQTARTVLLAVLLGLLVAGAALAYITVRVVNELRKAY
                     DKQQETARELAEVSQAKTDFIADVSHELRTPLTVLRGNAQVGLALGGDREYAELFEEI
                     VEESRRMTRLVEDLLFLARSDAASVPLQTETVDTVPLMHELAGRAESLARERGAKFES
                     VISGEGQVRVDAARIGQAVLILVDNATKYGTPGGPVTLSSGLTRTGELRVTVEDLGPG
                     ISPQELPHVFERFYRADKNRTRLGDVDGTGRGGTGLGLPIARTIVEAHGGR"
     gene            1402..2106
                     /locus_tag="AC16CM_02515"
     CDS             1402..2106
                     /locus_tag="AC16CM_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3138728.1"
                     /translation="MLILLIEDDPAIVRFLERGLAAHGHRTISADNGEDGLFMAAEEA
                     VELVLLDVMLPRMDGRRVLERIRLRRPDLPVLMLTARDGVHDKVEALDLGADDYLTKP
                     FDLEELLARMRALTRRSDQPWSSKIESGDLKVDLRSRKVWRGDRRMELSSREFALLEY
                     FMRHPGQVLSRQQILSAVWDYAFDPGSNVVDVYVRYLRSKLDRRGEPSVIETVRGAGY
                     RFEPPAARASSTEHQH"
     gene            2192..2761
                     /locus_tag="AC16CM_02520"
     CDS             2192..2761
                     /locus_tag="AC16CM_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138729.1"
                     /translation="MATKRISVAVLVGVLALAMAAPAAMAEERVCRGTIGATTVDNLL
                     VPQGAKCTLNGTRVEGTAKVERGAQLFANGVRVKGNVQSQGFQTVVLREASVVVGNVQ
                     LENGREGGSGRVINSRVNGDVQYFSNKARMVARGNTILGNLQANQNTGGLVISGNSIA
                     ENLQCQANKPRPTGGGNTAGDKEDQCAAL"
     gene            2858..3295
                     /locus_tag="AC16CM_02525"
     CDS             2858..3295
                     /locus_tag="AC16CM_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HIR3138730.1"
                     /translation="MATAERRANVVWKGNLFRGSGTFDLVSSEAVQGLPVTWASRTER
                     PDGKTSPEELIAAAHASCFAMAFSNTLAEQGNEPEELNVTAVCTFDADQVKVTSSVLD
                     VRGKVPGLDAEGFQNAAEQAEQGCPVSNALRGNVDIQVNASLD"
     gene            complement(3306..3521)
                     /locus_tag="AC16CM_02530"
     CDS             complement(3306..3521)
                     /locus_tag="AC16CM_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138731.1"
                     /translation="MKRLYSREELGRTVPLCGACHRQIHRTLTEKELEREYNTVGALL
                     SHPDISRFVRWIERKPHGVVARDDRTF"
     gene            complement(3828..4325)
                     /locus_tag="AC16CM_02535"
     CDS             complement(3828..4325)
                     /locus_tag="AC16CM_02535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138732.1"
                     /translation="MEITIRHAEPADYDALHRIFSGPLVVAGTLQLPFPSAEMWRRRL
                     AEPPEGLFSLVACLGDEVVGEISLHTHPTRWRMRHVGSIGMAVRDDRQGQGVGTALMR
                     AVLDLADNWLNLTRIELRVYTDNVPAIALYEKFGFEREGMHRRLAFRDGEYVDGYSMA
                     RVVNR"
     gene            complement(4392..>5945)
                     /locus_tag="AC16CM_02540"
     CDS             complement(4392..>5945)
                     /locus_tag="AC16CM_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute symporter family protein"
                     /protein_id="HIR3138733.1"
                     /translation="AWASRQNTDTSHQYVAGGEIKGWQNGLAISGDYLSAASFLGIAG
                     AIALTGFSGFYLSIGFLVAYLVVLLLVAEPLRNMGKYTLADMLASRFNASSVRSVSAL
                     STLAISMFYMIAQLNGSGALIGLLLGLPYWFSVIVIGILMTVYVVAGGMVATTWIQII
                     KALLLIAGTLTLSILVLARYGFNPFELFAAVENDIGREALAPPPPSGLTAGLDVISLN
                     IALVLGTAGLPHILIRFLTVPDAKTARSSIVTATWIIGLFYLLTPVLGYGAALIVGQE
                     AIANANAAGNLAAPQLAEALGGPIFFAFISAVAFSTIVAVVAGLVVAASSAFAHDFYT
                     NVIRGGQATEQEQFRAARYAAIGISVAAILLALPAESFNVAFLVALAFAVAASANVPV
                     ILLTIFWSRFNTAGAVTGMLVGLISAVGLVLLSPNVLTGPNPEPPLTPIIPIDAIFPL
                     TSPALVSVPLGFLGCYLGTIFGGRGAEREREQGLQTSYDEIRVRANTGFSDIEEEIHA
                     TAEEQHRTT"
BASE COUNT         1130 a         1872 c         1930 g         1013 t
ORIGIN      
        1 tgcgaccgcc gtgggcctcg acgatggtcc tggcgatagg caaaccaagc cccgtgcctc
       61 cccttcccgt cccatcgacg tctcccaggc gagtgcggtt cttgtccgcg cggtaaaagc
      121 gctcgaagac gtgtggcagt tcttgcgggg agatgccggg gccgaggtct tcgaccgtga
      181 cccggagctc gcccgtacgg gtaaggcccg aggagagcgt gaccgggccg cccggagttc
      241 cgtacttggt ggcgttgtca accaggatca gcacggcctg tcctatacgg gccgcatcca
      301 cccggacctg tccttctccg gagatcacgc tttcaaactt cgcgccgcgc tctcgggcga
      361 gtgattcggc cctcccggcc agctcgtgca tgagcggaac ggtatccacg gtctccgtct
      421 gcagtggaac cgaggcggca tcagaccggg caaggaacag aaggtcctcg accaggcgcg
      481 tcattcgcct ggactcctcc acgatctcct cgaaaagctc cgcgtactcg cggtcgccgc
      541 caagggcgag tccaacctgg gcgttgccgc gaagcacggt caggggcgtc ctcaattcgt
      601 gggagacgtc ggctataaag tcggtcttgg cctgggagac ctcggccagt tctctcgcgg
      661 tctcttgctg cttgtcgtac gccttcctaa gctcgttgac gacgcgtacc gtgatgtatg
      721 ccagggccgc tcccgccacg agtagcccca acaacacggc gagcaggacg gtcctggccg
      781 tctgcgtggc cgcgtctaac tccgacaacg acttctggga gagcgtctct ccgagatcgt
      841 cgagcgactc gccgatttgc gcgagttcgt ccaggcgatc caggcccctg gcgctcgccc
      901 tctcgaaggc gtcccgatcc gtttcgtaga gttctatcgc tggccggaag tcgcgatagt
      961 acctttccgc catcttccgg atctgcgctg gctgtgtaac atcctggtcg cgaaccccgg
     1021 tgttctccag ctcctctatc tcttcgccca gccggtcgta ggaggcttcg aagttgtcga
     1081 tctggacctc ggaaggcccg ctgaaggtta tgttgcgctg gtagtgacgc acgtcgagga
     1141 ccgcggcccg catgtcgtct ccctcgtctt ccagggcgag gtcgtagcga gcttcatcgg
     1201 tcacgccctc tacgcgcaag ttggacagga gtcccacgaa gccggcgata aagatcgcga
     1261 tcagtagccc cccgacggca gccagcgtga tccgccacgg ctgctctctt atccaaccga
     1321 tccacttcat gcttgcggac ctccacgtga tggctctgga gttttactgt cagaagctgt
     1381 cagattatac ttgtgggcgt catgctgatc cttctgatag aggacgatcc tgccatcgtg
     1441 cgcttcctgg agcgcggtct cgcggcccat ggccaccgca ccatcagcgc cgataacggc
     1501 gaggatgggc tgtttatggc cgccgaggag gccgtcgaac tggtccttct cgacgtaatg
     1561 cttccgcgga tggacggccg ccgggtgctg gagcgtatcc ggctgaggcg acccgacctg
     1621 cccgtcctga tgctaaccgc ccgcgacggg gtacacgaca aggtcgaggc cctcgatctc
     1681 ggcgccgacg actacctgac caagccgttc gacctggagg agttgctggc gcgtatgcgc
     1741 gccctcaccc gccgctccga ccagccctgg tcatcgaaga ttgagtccgg ggacctcaaa
     1801 gtggacctgc gctcccgaaa ggtctggcgg ggggatcggc ggatggaact ttcaagccgc
     1861 gagttcgcgt tgctggagta ctttatgagg catccgggcc aggtactctc gcggcagcag
     1921 atcctctccg ccgtctggga ctacgcgttc gacccgggct cgaacgtcgt cgacgtctac
     1981 gtccggtatc tgaggagcaa gctcgaccgc cgcggggagc cctcggttat agagaccgtc
     2041 cgcggcgccg gctaccgctt cgagccgccc gcggcccggg cttcttccac agagcaccaa
     2101 cattaacgat ctctaacctt cttatcatcg ggctctaacg gggcaccatt atcgttgagc
     2161 agcagtcgat gagagcagaa ggaggaagca ggtggctacc aagaggatct cggtcgcggt
     2221 actggtgggc gtgctggccc tggcgatggc cgccccggcc gcgatggccg aagaacgggt
     2281 gtgtcggggt acgataggcg ctaccaccgt ggacaacctg ctggtgcccc agggcgcgaa
     2341 gtgcacgcta aacggcacca gggtagaggg aaccgccaag gtcgagcggg gcgcgcagct
     2401 cttcgccaac ggcgtgaggg tcaaaggcaa cgtgcagagc cagggcttcc agaccgtggt
     2461 cctcagggag gcctccgtcg tcgtcggaaa cgtgcagctc gagaacggac gcgagggcgg
     2521 cagcgggcgg gtcatcaact ccagggtcaa cggcgacgtg cagtatttct caaacaaggc
     2581 ccgcatggtc gccaggggca acactattct cggtaacctt caggccaacc agaataccgg
     2641 cgggctcgtg atctccggca actccatcgc cgagaacctg cagtgtcagg ccaacaaacc
     2701 acgtccgacc ggcggcggca acacggccgg cgacaaggaa gaccagtgcg ccgcactctg
     2761 agccgaagac ctacatcgga atgtcggata cctgattagg agaccggaaa agcgggtagc
     2821 ctcctatctg gaaagacgaa tggataggag tggaagcatg gctactgcag aacggcgcgc
     2881 aaacgtggtg tggaagggaa acctgtttcg aggatcgggt actttcgacc tggtgagcag
     2941 tgaggccgtg cagggacttc cagttacgtg ggcctcgcgc acggagcgtc ccgacggtaa
     3001 gaccagccca gaggagctca tagccgcggc ccatgccagc tgcttcgcta tggccttctc
     3061 caacaccctc gccgagcagg gcaacgagcc ggaggagctc aacgtcaccg cggtgtgcac
     3121 tttcgatgcc gaccaggtga aggtcacctc gtccgtactc gacgtacgcg gaaaggttcc
     3181 cggcctcgac gccgaagggt tccagaacgc cgcggagcag gccgagcagg gctgcccggt
     3241 ctcaaacgcc ttgcgcggca acgtcgacat tcaggtcaac gcgagcctgg actagcatcc
     3301 gtcctctaga aagttcggtc gtcgcgggcc acaacaccgt ggggcttgcg ctcgatccaa
     3361 cgcacgaacc gagagatatc ggggtgggag agcaacgccc ccacggtgtt gtactcccgc
     3421 tccaactctt tctccgtgag cgtgcggtgt atctgccggt ggcaggcgcc gcagagcggc
     3481 acggtacggc ccagctcttc ccgactatac aaacgcttca cccgcttgtt cgagtgacgg
     3541 ctgcgcggga tcagatggtg ccgggttcgt cgctccacaa cccgcccaca caacccacaa
     3601 gcaccaccaa gatcaaaacc cctggccacc agcagattgt acccccaccc gaccgcacca
     3661 cggtagacgc cttcgcaagg actcggcagc gcgggaacga cggccatgga atggccgagt
     3721 tcttctctgg acaccccgaa ggcgaccagc caaaaagccg aaagcgaggc gaagccgaag
     3781 cgttgccgac gagcgagggc gttaggccga gcgtgctgtg agccgcgtta gcggttcaca
     3841 acgcgtgcca tggagtagcc gtccacgtac tcgccgtcgc ggaacgcgag acggcggtgc
     3901 ataccctctc tctcgaaccc gaatttttcg tagagggcga tagccgggac gttatccgtg
     3961 tagacccgca gctcgatgcg tgtgaggttc agccagttgt cggccaggtc gagcaccgcc
     4021 cgcatcaggg ccgttcccac gccctggcct tgccggtcgt cgcgtactgc catgccgatg
     4081 ctgccgacgt ggcgcatgcg ccagcgggtc gggtgggtgt ggagggatat ctccccgacc
     4141 acctcatcgc cgaggcaggc aaccagggag aacagcccct ccggtggctc tgcgagacgc
     4201 cggcgccaca tctccgccga gggaaagggc aactgcaaag tgccggccac caccagcggg
     4261 ccgctgaaga tgcggtgcag cgcgtcgtag tctgccggct ccgcatgtcg gatggtgatc
     4321 tccagggtcg gcctcgataa gcggtcagcc ttctgcagaa ggctgaccgc tgaaagctga
     4381 tcgctctctc ctcacgtcgt gcggtgttgt tcctcggcgg tggcgtggat ctcctcttcg
     4441 atatcgctga agcccgtgtt ggcgcggacc cggatctcgt cgtaggaggt ctggagtccc
     4501 tgctcgcgct ctcgctcggc gccccgaccc ccgaagatcg tgcctaggta gcatcccaga
     4561 aagcccaggg ggacggagac cagggcaggg ctcgttagcg ggaagatagc gtctatgggg
     4621 atgatcgggg tcaacggagg ctcgggattc gggccggtca gcacgtttgg gctgagaagc
     4681 acgaggccca cagcggagat caggccgaca agcatcccgg taacggcccc cgccgtgttg
     4741 aaccgcgacc agaagatcgt aagcaggatg acgggcacgt tggcgcttgc ggcgaccgcg
     4801 aatgccaggg cgacgaggaa agctacgttg aagctctccg ccggaagcgc aagaagtatc
     4861 gccgcaacgg agataccaat cgccgcgtag cgcgcagcgc gaaactgctc ctgctcggtg
     4921 gcctgtccgc cacggatgac gttggtgtag aaatcgtggg cgaaggcgct ggaggccgcg
     4981 acgacaaggc ctgcgaccac ggccacgatg gtggagaagg ctaccgccga gataaaggcg
     5041 aaaaagatcg gacctccgag ggcctcggcg agctgcgggg ctgcgaggtt gcctgccgcg
     5101 ttcgcgttgg cgatggcttc ctggccgacg atcagcgccg caccgtagcc cagcacgggt
     5161 gtcaggaggt agaagaggcc gatgatccag gtggccgtca cgatcgagga gcgcgccgtc
     5221 ttggcgtctg ggacggtgag gaaccggatc aggatgtgcg gcaagccggc cgtcccgagc
     5281 accagggcga tgttgagcga gatcacgtcg agccctgcgg tcagcccgct tggcggcggt
     5341 ggagccaggg cctcgcgccc gatgtcgttc tcgaccgcgg cgaacagctc gaaggggttg
     5401 aacccatagc gggccagcac gaggatagag agggtcaggg taccggcgat caggagcaag
     5461 gccttgatga tctggatcca ggtcgtggcg accatgccgc ctgccacgac gtatacggtc
     5521 atcaaaatcc ctatgacgat gaccgagaac cagtatggca acccgagcaa gagcccgatg
     5581 agcgcccccg agccgttgag ttgcgcgatc atgtagaaca tgctgatcgc aagggtgctc
     5641 aaggcagaca ccgagcgaac gctactcgcg ttgaaccggc ttgcgagcat gtcagcaagg
     5701 gtgtacttgc ccatgttcct caggggttcg gctaccagga gcaacaccac aaggtacgcc
     5761 accaggaagc ctatggagag gtagaagcct gagaacccgg tgagggctat ggcccccgcg
     5821 atgccgagaa acgaggccgc cgacagatag tcgccggaga tagcgaggcc gttctgccag
     5881 cccttgatct caccgccagc cacgtactgg tggctcgtgt cggtgttctg gcgcgacgcc
     5941 caggc
//
LOCUS       DBNGVD010000083         2278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_564305, whole genome shotgun sequence.
ACCESSION   DBNGVD010000083 DBNGVD010000000
VERSION     DBNGVD010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_564305"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="AC16CM_02545"
     CDS             <1..863
                     /locus_tag="AC16CM_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138734.1"
                     /translation="TGVTMDPAGISDPEELRARLGMKEDRIRELYEEITAFRLAADEA
                     QAASKAGEAHIESLESESARLKERIRSLEEEARGRKRRRDGSEKQIARLEKALEKKDG
                     EVTHRDHLLERRAEQLEALSRESREQASRKDLALQDALRRVDGLERDLEGRENEILAL
                     RSTIESVRGDLEGERELRGRLSDPANRLRAGIDLFNESGQRRSMSSLSKTMGPPEVHV
                     ELTPGDAPLVLLTFTWQGVTWQTYVADPGPDVEEPRVYLKGAGEDLSGVDRNPPNARV
                     GPGEKVMLGL"
     gene            complement(914..1090)
                     /locus_tag="AC16CM_02550"
     CDS             complement(914..1090)
                     /locus_tag="AC16CM_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3138735.1"
                     /translation="MPVGPLEILLVFLVFLILGPRRIVNMFRSLGTGINDFVETLGRD
                     KKSELPEEDKDEEK"
     gene            complement(1090..1530)
                     /locus_tag="AC16CM_02555"
     CDS             complement(1090..1530)
                     /locus_tag="AC16CM_02555"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3138736.1"
                     /translation="MKRSHGGSVRIEQQVAAPPETVSAYVSDFRHARDWMVGVENVER
                     LGENDYKLTIDSPIGKIEPGVRIVENGPESISWVYTSTVEGGGRVDVIPEAGGCLVSY
                     TGEFHLKRRFLDRAARFVGAERFARTNGERSLARLKYLMEARRY"
     gene            complement(1527..1904)
                     /locus_tag="AC16CM_02560"
     CDS             complement(1527..1904)
                     /locus_tag="AC16CM_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3138737.1"
                     /translation="MGDVNRPTLDFYQLVERALDGLPPDLAELLDNVAIVVEDWPDRE
                     TQLSSDDPHDTLYGLYEGVPLTERGDSYYGVLPDRITIFRGPLERDFRAGELEEQVRI
                     TVVHEIAHYFGFGEDRLEELGWG"
     gene            1985..2227
                     /locus_tag="AC16CM_02565"
     CDS             1985..2227
                     /locus_tag="AC16CM_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138738.1"
                     /translation="MRFMSGSRPGRWVVWVLYRALNKPLPVPHAAEWMMIPEHSVDQV
                     LETGALRSLYPQDVLEAARRMDRQRREHDERYAEDR"
BASE COUNT          394 a          730 c          758 g          396 t
ORIGIN      
        1 ccaccggggt gacgatggat cccgcgggga tctcggaccc cgaggagttg cgggccaggc
       61 tcgggatgaa ggaagacaga atccgcgagc tgtacgagga gatcaccgcg ttccgcctgg
      121 ccgccgacga ggcccaggcc gcgagtaagg cgggtgaggc acacatcgag tccttagaga
      181 gcgagagcgc gcggctgaag gagcgcatcc ggagcctgga agaggaggct cgtggtcgca
      241 agcggcgcag ggatggctcg gagaagcaga tagcccgcct ggagaaggcg ctggagaaga
      301 aggacggcga ggtcacccac cgcgaccacc tgctggagcg tcgggccgag caattggagg
      361 ccctcagccg tgaatccagg gagcaggcct cgcgcaagga tctggccctg caggacgccc
      421 tgcgacgcgt cgacggcctg gagagggacc tggaggggag ggagaacgag atccttgccc
      481 tccggtctac catcgagtcg gtgcgtggcg acctggaggg cgagcgggag ctgcgtggac
      541 gcctctccga cccagccaac cggctgcgcg ccggcatcga cctgttcaac gagtcgggcc
      601 agcgccgctc catgagctcc ctctcgaaga cgatgggccc acccgaagtc cacgtggagc
      661 tcaccccggg agacgcaccg ctggtcctgc ttaccttcac ctggcagggc gtcacctggc
      721 agacctacgt cgcggacccg ggacccgatg tggaggagcc gcgcgtctac ctgaagggcg
      781 cgggagaaga cctgtcaggg gtggaccgca acccccccaa cgcccgtgtg gggcccggcg
      841 aaaaggtgat gctcgggttg tagtttcggc tctcggcttc cagcgggcag ctttcggcat
      901 gtagccggag gctttacttc tcctcgtcct tgtcttcttc gggaagctcg ctcttcttgt
      961 cgcggcccag ggtttccacg aagtcgttta tgccggtacc cagcgagcgg aacatattga
     1021 cgatgcggcg tgggccgagg atgagaaaga ccaggaacac gagcagtatc tccaggggac
     1081 caacgggcat cagtagcgtc tggcttccat gaggtatttg aggcgcgcca gggagcgttc
     1141 gccgttggta cgggcgaagc gttcggcgcc cacaaagcgg gcggcgcggt ccaggaaacg
     1201 gcgtttgagg tggaattcgc ccgtgtagga gaccaggcag ccccccgcct cggggatgac
     1261 gtcaacccgc ccgccgccct ccacggttga ggtgtagacc cagcttatgc tctcggggcc
     1321 gttctccacg attcgcaccc ccggctctat cttgccgatg gggctgtcga tggtcagttt
     1381 gtagtcgttc tcccccaggc gttcgacgtt ctctacgccg accatccaat ctcgggcgtg
     1441 acggaagtcc gagacgtagg ctgatacggt ttcgggcggt gctgccacct gctgctcgat
     1501 gcgcacgctg ccgccgtgag aacgcttcac ccccaaccca gctcctccag ccgatcctcg
     1561 ccgaagccga agtagtgcgc gatctcgtgc accaccgtga tcctgacctg ctcctcgagc
     1621 tcaccggccc ggaaatcccg ctccagcggg ccgcggaaga tagtgatcct gtcgggcaag
     1681 acgccgtagt acgagtctcc tcgctccgtt agaggcaccc cttcgtacaa cccgtacagg
     1741 gtgtcatgcg gatcgtcgga ggagagttgt gtttcgcggt ctggccaatc ctcgaccact
     1801 atggccacgt tgtccagaag ctccgcgaga tccggcggca gcccgtcgag tgcccgctcc
     1861 acgagctggt agaaatctag ggtcggcctg ttcacatcgc ccattgtact tcagccccgc
     1921 cgcgtggtgg cggtccggca cgttagaata ggctctcttt acgacgtttt gcggaggtgt
     1981 gcgcttgagg ttcatgtctg gttctcgtcc ggggcggtgg gtggtctggg ttctctaccg
     2041 ggccctcaac aagccgcttc cggtaccgca cgcggcggag tggatgatga tacccgagca
     2101 ctccgtggat caggtcctgg agaccggcgc tttgcgtagc ctctacccgc aggacgttct
     2161 cgaggccgcc cgccgcatgg accgccagcg ccgcgagcac gacgaacgct acgccgaaga
     2221 ccggtagaac cttcgcccgt catcccgcgc cccctttcgc tctggggatg cgtcaccc
//
LOCUS       DBNGVD010000084         5701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2204351, whole genome shotgun sequence.
ACCESSION   DBNGVD010000084 DBNGVD010000000
VERSION     DBNGVD010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2204351"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="AC16CM_02570"
     CDS             <1..1062
                     /locus_tag="AC16CM_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138739.1"
                     /translation="LYPLVPYFEDELGLSKSVVGLLSGAYGAGVLAGSVPGGYLASRS
                     GVRITAVLGLTLMSVTSVVFALADSTWLLVLARFTGGFGSALSWVAAFTWLVDRAPEE
                     RRAELIGVMISSAVVGALLGPVLGSVAATVGLLPAFSAVALAGLAIALWAAFEPASAS
                     TGTVSLLPALGMLRSPLLLTSLWFIVLSPLLFSALAVLVPLDLHRLGWGAAAVGVVFL
                     LSAIFEAFVHPMLGRWSDRSGYRRPVIVGLISSIGILLLLTWVDNPWVIGLLVVLAAG
                     AFNATLVPGTALFSRGTEKAGMDRAVAFAMTNFAWASGYAVGAPVGGFLADLKGDALS
                     YLTLTVVCLLTILALRRTT"
     gene            complement(1088..1246)
                     /locus_tag="AC16CM_02575"
     CDS             complement(1088..1246)
                     /locus_tag="AC16CM_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HIR3138740.1"
                     /translation="MDLLRIIIAIILPPLGVFLQVGIGKQFWINILLTILGYIPGIVH
                     AVWVIAKY"
     gene            1390..2100
                     /locus_tag="AC16CM_02580"
     CDS             1390..2100
                     /locus_tag="AC16CM_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HIR3138741.1"
                     /translation="MSLMAQPIDLYYWPTPNGWKITIFLEEAGLPYSIVPVDITAGDQ
                     YEEEFLEISPNNKMPAIVDPEGPDGEPISLFESGAILLYLADKTGEFIPESERDRYTV
                     LQWLMFQMGHVGPMLGQAHHFRGYAPEEVPYAIDRYTNEATRLYGVMDRRLSETEYFA
                     GEEYTIADMAIFPWLISHERQGQDLDDYPNVKRWFETIESRPAVARAREVGQELRRSL
                     EDMDDDTRKALFGGPPRR"
     gene            complement(2501..3940)
                     /locus_tag="AC16CM_02585"
     CDS             complement(2501..3940)
                     /locus_tag="AC16CM_02585"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3138742.1"
                     /translation="MGTAVLLTSAVISIVVLGLVRSLADAAEPPNVVLVVTDDLTKKD
                     YLELGNNLGFFTSGGTFFHNAFVTTALCCPSRASALTGLYAHNHGITQHIDPGTGYEQ
                     YHAEGYDQNDLPVWLEDSGYRTGLVGKYMNKYDAQHDGVPAGWTDWYGADDPAVRWTL
                     NENGSIRTYKQDPTAPAYEPWEDVLGDRALKFVDEAHASGKPFFLWYGTHAPHSPELI
                     APQDEERVGTWPTYDPPGFNERDVSDKPRWVRDRPLLASTRIRALKQQRQERLTSMLA
                     VSRNLARLKDKLRQTGELSNTYIILTSDNGYHQAHHRLGPGKMTAYEEDMRVPLAVSG
                     PGVAPGSVKHTVLNTDLAPTIADLAGVTPGLVPDGRSFAPLLPKGRSGIAADQFRRRF
                     MEENWQSPVPTPTGPELFPAPSNFALRGSDYIYNRYETGETEYYDLVRDPYELESKQI
                     GGTRKENLDTKWTRLINCRGAQCQTAEGG"
     gene            4353..4910
                     /locus_tag="AC16CM_02590"
     CDS             4353..4910
                     /locus_tag="AC16CM_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3138743.1"
                     /translation="MFTVGHSNHSADNFLGLLRGRGIEVVVDTRSHPYSRHAPHFNAH
                     PLEAALAGAGIGYQFLGGELGGRPEGGEYYDDLGRVDYALVGCSRPFLDGISRLQKEI
                     LANRVALLCSEENPVRCHRRLLVGRALEELGFTLRHIRGDGSVQVEGEANGGQPELFP
                     ELEASPRKSIRSASPRKPHRSSSAP"
     gene            4907..5284
                     /locus_tag="AC16CM_02595"
     CDS             4907..5284
                     /locus_tag="AC16CM_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3138744.1"
                     /translation="MNDSDVKRLLDVRLNNASQLAGFARRADLPYFLRELCGAESVYE
                     PALAPTREILGAYRGGGGWQAYEKAFLGLLRERRVEEVLHRDIFDVPTVLLCSEARPE
                     HCHRRLVLEYLNREWGGIKAVHL"
     gene            5290..>5701
                     /locus_tag="AC16CM_02600"
     CDS             5290..>5701
                     /locus_tag="AC16CM_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138745.1"
                     /translation="MRIRVDHLTRMQPGYFCAAGIDLETGEHVRPVLRRGRLTTDLLS
                     TNKGPFDIGSVLDLGTTTDVGRAPELEDRRFDPADVRWLRDEEPYEYWNALERASRES
                     LEDIFGPALELWDESGTVDVGEGLASLGLLRPEKQ"
BASE COUNT         1012 a         1713 c         1826 g         1150 t
ORIGIN      
        1 ctctacccgc tcgtcccata tttcgaggac gaactaggcc tctccaaatc ggtagtcggc
       61 ttgttgagcg gcgcctacgg agccggtgtt ctcgccggca gcgtgcccgg cgggtacctc
      121 gcttcgcggt cgggggtcag gatcaccgcg gtcttgggcc tgaccctgat gtccgtgacc
      181 agcgtggtat tcgcgctcgc cgacagcacg tggttgctcg tgctggcccg cttcaccggc
      241 gggttcggca gcgccctttc ctgggtggcg gcgttcacct ggctcgtgga ccgggcccct
      301 gaggaacgtc gggccgagct gatcggggtg atgatcagct ccgcggtagt gggagcgctg
      361 ctcgggcccg tgctggggag cgtagccgcc accgtgggcc tgcttccagc gttttcggcc
      421 gtcgccctgg cggggttggc catcgccctc tgggccgcct tcgagcccgc atcagcctcc
      481 accggaaccg tgtccttgct gccggcactc gggatgctga ggagcccctt gctccttacc
      541 agcctgtggt tcatagtgct ctccccgctg ctcttcagcg cgctggccgt cctggtgccc
      601 cttgacctcc accggctcgg atggggtgcg gcggccgtgg gagtggtgtt cctcttgagc
      661 gccatcttcg aggccttcgt ccacccgatg ctcggccggt ggtccgaccg ctcgggttac
      721 cgtaggccgg tcatcgtagg tttgatctcc tctatcggga ttctgcttct cctcacctgg
      781 gtcgataatc cctgggtgat cgggcttctc gtcgtgttag cagcaggtgc cttcaacgcc
      841 accctggttc cgggcacggc gctcttctcg cgcggaacgg agaaggcggg gatggacagg
      901 gccgttgcct tcgccatgac gaacttcgcc tgggcttcag gctacgccgt gggagccccg
      961 gtaggcggct ttctggcgga tctgaagggc gacgccctct cctacctcac gctcaccgtg
     1021 gtctgcctgc tgacgatcct cgcgctccgc cgcaccactt gaagctgacc gctgaaagct
     1081 gaaggctcta gtacttcgct ataacccaga cggcgtggac tatgccgggg atataaccga
     1141 ggatggttag cagaatgttg atccagaact gtttaccaat ccctacctgc aagaacaccc
     1201 ctagtggcgg cagaattata gcgataatga tgcgtagtag atccatctag aaacctctct
     1261 ctcgctctta cctgtcctgt acgttctccg gtgctggtgg ttgcagcctc gaatatatac
     1321 ggatacgagg tttcgattgc ctgcgtgcgg gagaactagg tccatggtac acgaaacgag
     1381 attgcgagga tgagcttgat ggcgcaaccc atagacctct attactggcc gacgcccaac
     1441 gggtggaaga tcacgatctt ccttgaggag gctggactcc cgtatagcat agtgcccgtg
     1501 gatattaccg ccggcgacca gtacgaggag gagtttctcg agataagccc caacaacaag
     1561 atgccggcca tcgtagaccc ggaaggtccc gacggtgaac ctatctcgct cttcgaatcc
     1621 ggggccatcc tgctctacct cgccgacaag acgggcgaat tcataccaga gtccgagcgg
     1681 gacaggtaca cggtactgca gtggctgatg ttccagatgg gtcacgtggg gccaatgctc
     1741 ggacaggccc accatttcag gggctacgcg ccggaagagg tgccctacgc gatagatcgc
     1801 tacaccaacg aggccacgcg cctctacggc gtcatggacc ggcgcctctc cgaaacggag
     1861 tacttcgcgg gcgaagagta cacgatcgcg gacatggcga tcttcccgtg gctcatctcc
     1921 cacgaacgtc agggtcagga cctcgacgac tacccgaacg tgaaacgctg gttcgagacc
     1981 atagaatcac gtcccgcggt ggcaagggcc cgcgaggtcg ggcaggaact gcgccgctcg
     2041 ctggaggata tggacgacga cacccgcaag gcgctcttcg ggggtccgcc gaggcggtag
     2101 gaccaacccg ttttcgtacg gggtcgcggc gcccgaggga gacggcgcat cgtggggcgg
     2161 ttcgcgaacc gcccctacat gggttgtggg agattcgtaa cgccacggat tgcggatcgc
     2221 ttctagcggg tcttgtctct ggttacacct gtgccgaaaa gtcgcgggtc gaacccctga
     2281 tgcctgtggc ctgtttccgc gaccagcggt cctcggtgac ctctatctct gctagttcta
     2341 gggtggcggc gtggccccgc taccctatca gatgtgtagg gcaacagagc cttgatcccg
     2401 ggcaacggac acggcgacca cagaccatgg cagcggccga gccgaacgta agacgaggtt
     2461 tacacttggt ctggaagcgt aaggtggagc aatcgggtaa tcagccccct tccgccgtct
     2521 ggcactgggc accccggcaa ttgatcagtc gtgtccattt tgtatccagg ttctccttgc
     2581 gtgttccgcc gatctgcttg ctctccagtt cataaggatc gcgcaccaga tcgtagtatt
     2641 cggtctcccc agtctcgtac ctgttataga tataatccga gcccctgagg gcgaagttgg
     2701 atggcgcagg gaagagctcg ggtcccgttg gcgtcggaac ggggctttgc caattctcct
     2761 ccatgaacct tcttctgaac tggtcggcag cgatgcccga acgtcccttt ggaagcagtg
     2821 gagcgaagga gcgcccgtcg ggaacaagtc cgggcgtgac accagcaagg tcggcgatgg
     2881 tcggtgcaag gtcggtgttc aacacggtat gtttcacgct gcctggggcg acaccgggtc
     2941 ccgagaccgc cagcgggaca cgcatgtcct cctcgtaggc ggtcatcttt ccgggaccaa
     3001 ggcgatgatg ggcctggtga tagccgttgt ctgaggtcag gataatgtag gtgttggaga
     3061 gttcgccggt ctgtctgagc ttgtccttta gcctcgcgag gttgcgggag acggcgagca
     3121 tcgaggtaag gcgctcctgg cgttgttgct tgagcgctcg tatccgtgtg gaagcgagga
     3181 gtggccgatc cctcacccat cttggcttgt ccgagacgtc cctctcgtta aagccgggcg
     3241 ggtcgtaggt tggccaggtc cccacccttt cctcgtcttg cggggcgatg agctcggggg
     3301 agtgaggtgc atgggtcccg taccagagga agaacggttt tcctgaagcg tgggcttcgt
     3361 cgacgaactt cagagccctg tcaccgagca cgtcctccca gggctcgtag gccggcgcgg
     3421 tcgggtcctg cttataagtt cttatgctgc cgttctcgtt tagcgtccaa cggacggcag
     3481 ggtcgtcggc gccataccag tccgtccatc ccgccggcac cccatcgtgc tgcgcgtcgt
     3541 acttgttcat gtacttgccg acgagacccg tcctgtagcc cgagtcctca agccacaccg
     3601 gtagatcgtt ctgatcgtac ccttcggcgt ggtactgctc gtagccggta cccgggtcta
     3661 tgtgctgggt tatgccgtgg ttgtgggcgt agagaccggt cagggcagaa gcccgcgaag
     3721 ggcagcacag agcggtggtc acgaacgcgt tgtggaagaa cgtccctcca gaggtaaaga
     3781 accccaggtt gttaccgagt tcgaggtagt ctttcttggt cagatcgtcg gtgaccacga
     3841 gtactacgtt cggcggttct gcagcatcgg ccagggatct caccaggcca agaaccacga
     3901 tggagatgac cgcgcttgtc aggagcaccg ctgtgcccat cgaagccagc aatcgaacca
     3961 tttctgcctg cgccttcctc cagtatggtg tacgaggttg tcttgtatgt tcccatccca
     4021 acgcacgtaa cggacgtatc gtaacaaact tgcgaggagt agcacagccc cagataggtt
     4081 gtccgcggac cattgcgtga tctcgatgga ccataagtac cggtgatccg gggagccccg
     4141 acggggtgcc gggtgatcta tgctcgcgag aatatacccg ctatcccttc ttctggcgtt
     4201 tgtattccgg gcagacgaag gttacagcgc ccgtggtcgg cgaaagtggg tctggggagc
     4261 gagcgacttc gggattatgc tcggtaacgt tcgccgtgtt tcgcagaaat ggagggactg
     4321 tcataggaga ccaggagcct ggtaagaacc cggtgtttac cgtaggccac tccaatcatt
     4381 cggcggataa tttccttggc ttgctgcggg gacgtgggat cgaggttgtc gtggacaccc
     4441 gctcgcaccc gtactcaagg cacgcaccgc atttcaacgc ccaccctctc gaggcagcgc
     4501 tggcgggggc aggcatcggt tatcagtttc tcggtgggga gctcgggggg aggccagaag
     4561 gcggggagta ctacgacgac ctcggtcggg tggactacgc gctggtcggg tgttcgaggc
     4621 cgtttctcga tggtataagt aggcttcaga aggagatcct tgccaacagg gtcgcactgt
     4681 tgtgcagcga ggaaaaccct gtccgttgcc accgcaggct cctcgtcggt agggccctgg
     4741 aggagctagg gtttacgctt cggcacatca ggggcgacgg cagcgtgcag gtagagggcg
     4801 aggcgaacgg cggacagccg gagttgttcc cggagctgga ggcaagccca cggaagtcta
     4861 tacgatcggc ttcaccaaga aaaccgcatc ggagttcttc ggcaccttga acgactcgga
     4921 cgtcaagcgc ctcctagacg tgcgcctcaa caacgcctcc cagctcgccg ggttcgcccg
     4981 ccgcgccgac ctcccgtact ttctgcggga gctctgcggc gcggaatccg tctacgaacc
     5041 cgcgctcgcc ccgacacgtg agatcctggg cgcctacaga ggcggcggcg ggtggcaggc
     5101 ctacgagaaa gcgtttctgg gcctgcttcg ggagcgtcgg gtagaagaag tgctccacag
     5161 agacatcttc gatgttccga cggtgctgct gtgcagcgag gcaagacccg agcactgcca
     5221 ccggcgcctg gtactcgagt acctgaaccg cgagtggggc ggcatcaagg ccgtccacct
     5281 ctaggtagca tgcggatccg cgtagaccac ctgacgcgca tgcagccggg gtacttctgc
     5341 gccgccggca tagacctcga aaccggggag cacgtccgcc cggtcctccg ccgcggcagg
     5401 ctgaccaccg atctgctcag caccaacaaa ggccccttcg atatcgggtc cgtcctagac
     5461 ctcggtacga caactgacgt cgggcgagcc ccagagctgg aggacaggcg cttcgacccg
     5521 gccgacgtga gatggttgcg tgacgaggag ccatacgagt actggaacgc cctcgaaagg
     5581 gcgtcgcggg agagcctgga ggacatcttc gggccagcgc tcgagctgtg ggacgagagc
     5641 ggcacggtcg acgtcggcga ggggctggca tcgcttggcc tcctccgtcc cgaaaaacag
     5701 c
//
LOCUS       DBNGVD010000085         2577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2569169, whole genome shotgun sequence.
ACCESSION   DBNGVD010000085 DBNGVD010000000
VERSION     DBNGVD010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2569169"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="AC16CM_02605"
     CDS             <1..836
                     /locus_tag="AC16CM_02605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3138746.1"
                     /translation="KGRAGGLRKFVSEFLEDRGVRPVWHITEGPGHAGAIVGELPEGA
                     PVVAVGGDGTVHEVAEACVESGRVMGILPAGSGNDYVKALGVGTRLRRALAVLVSGRV
                     RVVDTAEVDGVPFNNGLGIGFDAEVAAGVALAPAYLGGTGRYLWSVGRLLKDFRCHEA
                     RLTLDGRVIEARTILVAVALGTTYGSLFRLTPDAVLDDGLFDVIWSEEVNRAEVLRLI
                     PAALRGTLAERRKVHTARASEVEIVLGEEVPAHVDGEMLADTRHFRARVLPGTLRVVA
                     P"
     gene            965..1813
                     /locus_tag="AC16CM_02610"
     CDS             965..1813
                     /locus_tag="AC16CM_02610"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3138747.1"
                     /translation="MRAFVFPGQGGGVVEPPPETVPGIKRVVGERVPDQVKIFARAAH
                     DADESRVMEVRPDVVAGHSLGEYGAAYAAGCFDFETGLWLVAERDRFLAEAAEENPGA
                     MVAILRTDPSEIEAALGDEGPVIANYNSPRQTVVSGLRDDLGEAVSRIKGRKVPLNVS
                     FAAHSPYVAAAGEKMRDVLDTIEFRAPRVPVISAMNGAVHTGAAEVKEALKGQMVAPV
                     RWVNVVETLARMRVEEWVELGGGGVLTRMLRDFELPSLQGITFEDLRTRLYGQVQSVL
                     TRKEDE"
     gene            1976..>2577
                     /locus_tag="AC16CM_02615"
     CDS             1976..>2577
                     /locus_tag="AC16CM_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3138748.1"
                     /translation="MSGLTIPYWEVALRLGLAVLLCGAIGFEREVRDQPAGFRTHILL
                     GLGAALFTLVSAYGFQPFTQAALSGGGLQFDPTRIAAQIVAGVGFLGAGAIIRQGQDV
                     RGLTTAASLWAVSAIGMAIGAGYLFGAATATVLAVATLNALRRFRSSVINPMRLDSAD
                     LELELGTTAEGPTEALRVLERHDITVRSTDAEIDQERSRY"
BASE COUNT          422 a          798 c          927 g          430 t
ORIGIN      
        1 gcaaaggacg ggccgggggg ttgcggaagt tcgtctccga gtttctggag gatcgggggg
       61 tgcggccggt ctggcacatc acggagggtc ccggtcacgc tggcgccatc gtgggggagt
      121 taccggaggg ggcgcccgtc gtggccgttg ggggggatgg gaccgtacac gaggttgcgg
      181 aggcgtgtgt ggagtcgggc cgggtcatgg gcatcctgcc ggccggcagc ggcaacgact
      241 acgtcaaggc cctgggtgtt ggcaccaggc tgcggcgggc gctggctgtt ctcgtgtctg
      301 gcagggtaag ggtcgtcgac accgccgagg tagacggggt tcccttcaac aacgggctcg
      361 gcataggctt tgacgccgag gtcgctgccg gcgtcgccct ggcgccggct tacctggggg
      421 gtacgggacg gtatctgtgg tcggtcggga ggttgctcaa ggattttcgc tgccacgagg
      481 ccaggcttac gctagatggc cgggttatcg aggcaaggac catactcgta gcggtggccc
      541 tgggcaccac gtacggttcg ctgttccggt tgacccccga cgccgtgctc gacgacggcc
      601 tgttcgacgt catctggtcc gaggaggtca accgggccga ggtcctccga ctaatcccgg
      661 cggcgctaag gggcacgctc gccgagcgcc gcaaggtgca caccgcaagg gcgagtgagg
      721 tcgagatagt actaggcgag gaggtgcccg cccacgtcga cggggagatg ctcgccgaca
      781 cccgccactt ccgggcgcgg gtgctgcccg gaacgctgcg cgtcgtggca ccttgaagcg
      841 aggcttcccg gtgcggccgg tcctcacccg tgccgtcgat acggcgtact tgtggtaaaa
      901 agctctatct acaccttcgg ggtagggtcg tatctcccaa cacatcgagg tcaggcggaa
      961 acacatgaga gccttcgtat ttcccgggca gggcggcggg gtggtcgagc ccccgcccga
     1021 aacggtgccg ggcatcaagc gcgtcgtcgg agagcgcgtc ccggatcagg tcaagatctt
     1081 cgcccgtgcg gcacacgacg cagacgagag ccgcgtcatg gaggtgcgcc ccgatgtggt
     1141 ggcggggcac tctcttgggg agtacggcgc ggcctacgcg gcgggctgct tcgacttcga
     1201 gacgggcctc tggctcgtgg ccgagcgcga ccggttcctc gccgaggccg ccgaagaaaa
     1261 ccccggcgcg atggtcgcca tcttgagaac ggacccatcc gagatcgagg cggcgcttgg
     1321 cgatgaaggt cccgttatag ccaactacaa ctccccgagg cagaccgtgg tctcggggct
     1381 gcgcgatgac ctcggggagg ccgtctccag gataaagggt cgcaaggtac cgctaaacgt
     1441 ctccttcgcc gcccactccc cgtacgtggc ggcggccggc gagaagatgc gcgacgtcct
     1501 cgacaccatc gagttccgcg cgccgcgggt ccccgtgatc agcgccatga acggtgccgt
     1561 gcacacgggc gcagccgagg taaaggaagc cctcaaagga cagatggtgg cccccgtccg
     1621 ctgggtcaat gtcgtcgaga cgctggccag gatgagggtg gaagagtggg tagagctggg
     1681 tgggggcggc gtcctgacca ggatgctgag ggacttcgaa ctgccctcgc tccagggtat
     1741 cactttcgag gatctgcgaa cgcggctgta cggacaggtc cagagcgttc tcacacgcaa
     1801 agaggatgaa taggctacag gcatcaggct acgggtgtca ggtcttccga ccgcatccct
     1861 acggcgcagc gcgaatctgg atgcagaggc ctttcgaggg tgggacagca cgaccgggtg
     1921 ccgtgtacac gggccttcct gaaacctgtg acctgaaacc tgtagctttc acatcatgtc
     1981 cgggctgacg ataccttact gggaggtcgc gctgcggctc gggctggcag tgttgctgtg
     2041 cggcgccatc ggtttcgagc gggaggtccg cgaccagccg gcggggttca ggacgcacat
     2101 cctgctcggc ctcggggcgg cgcttttcac cctcgtctcg gcctacggat tccagccgtt
     2161 cacgcaggcg gcactaagcg gcgggggttt gcagttcgac ccgaccagga tcgccgccca
     2221 gatcgtggcc ggcgtcggct tcctcggggc cggggcgatc atcaggcagg gccaggacgt
     2281 gagggggctc accaccgcgg cgagcctctg ggccgtgagc gccatcggca tggccatcgg
     2341 ggcaggctac ctgttcggcg cggccaccgc tacggtcctc gccgttgcca cgctgaacgc
     2401 cctgcgccgc ttccggtcct ccgtcatcaa ccccatgcgt ctcgactccg ccgacctgga
     2461 actggaactg ggcacgacgg cggagggccc cacagaagcg ttgcgggtgc tcgaacgtca
     2521 cgacatcacc gtgcgtagca cggacgccga gatagaccag gaacggtccc gctacaa
//
LOCUS       DBNGVD010000086         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_929812, whole genome shotgun sequence.
ACCESSION   DBNGVD010000086 DBNGVD010000000
VERSION     DBNGVD010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_929812"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC16CM_02620"
     CDS             <1..268
                     /locus_tag="AC16CM_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138749.1"
                     /translation="LNTACSRTDRQFKPLIEARVQAELYELLLRWIRASPVDARSADA
                     EITATVVSWAIFGAGLRWSQGGEPGSLEEYADRALSAIVGGLGS"
     gene            446..1060
                     /locus_tag="AC16CM_02625"
     CDS             446..1060
                     /locus_tag="AC16CM_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3138750.1"
                     /translation="MLRKTMLLATVVALVLSVAATALASSPGDGKAHARESMDKNELR
                     KKEEFAGLVDIGGGRQVYMECRGKGRPTAVLVSGGAGSHEDWTHLIDAEGGLEPSGSA
                     VFPQAGKFTRVCVYDRPGTLRFDGTLSPSTPVQQPTTASDGAADLHALLEASRQKDPH
                     VLVAFSWGGMIARQYVSDYPDEVSGLVLVDPASEFLQDTLTPSQ"
     gene            1076..1393
                     /locus_tag="AC16CM_02630"
     CDS             1076..1393
                     /locus_tag="AC16CM_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138751.1"
                     /translation="MGTHPPEGIEGVDHGSSAPTLRASPQVRGIPSVVLSSDECFELL
                     PGENTCQAWREAQDLLVAQLGAEQITRTDCSHPIQLQNPEIVIDSVRQVVEAARKKSS
                     ALG"
     gene            complement(1938..2579)
                     /locus_tag="AC16CM_02635"
     CDS             complement(1938..2579)
                     /locus_tag="AC16CM_02635"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR3138752.1"
                     /translation="MRSTLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRDILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDDNPEKIGRTVGNVVVKHIDELKKSVTEAEEIIGIIATPASSAQEV
                     ADLMVDSDIRVILNYTDVLLHVPTHVDVHRIDPTAQLMHTLYYLTQAEGQEAS"
     gene            2849..3814
                     /locus_tag="AC16CM_02640"
     CDS             2849..3814
                     /locus_tag="AC16CM_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HIR3138753.1"
                     /translation="MEYRRLGGSGVRVSEISLGSWLTYGGSVAEEQATACIDRAYEIG
                     INFFDTANVYRRGAAEEIVGRALRKFDRDSYFLATKVYFPMGEGPNDRGLSRKHITEQ
                     CHASLRRLGVNYVDLYQCHRYDENTPLEETLRALDDLVRQGKVLYVGVSEWTADQISD
                     ALRIAGEMNLDRIVSNQPQYNMIQRRIEPDVIPLCEREGVGQVVFSPLAQGVLTGKYR
                     PGDAPEEGTRAADPENNVFMQQLMNEPVLSAVDGLRSVASDSGLSVPQLALAWVLRQE
                     DVSSAIIGASRPEQVDDNAAASGVKLSADTLSEIDRILDGVTRFE"
BASE COUNT          687 a         1165 c         1228 g          740 t
ORIGIN      
        1 cctcaacacg gcctgctcga ggacggacag gcagttcaag ccgctcatag aggccagggt
       61 tcaggccgag ctatacgagc tcctgctgcg ttggatccgg gcctcgcccg tggacgctcg
      121 ttccgccgac gcggagatca cggccaccgt ggtcagctgg gctatcttcg gcgcggggct
      181 gcggtggagc cagggtgggg agccgggaag ccttgaggag tacgcggata gagccctctc
      241 ggccatcgtc ggagggcttg gatcctgaat ctagacccga ttcgttgtgt ggccaacttc
      301 gcggaggtca gatctttgaa acgtccttgt acctagccct tttgggggag gcgcccttac
      361 ctcgcagagt gtagtcttgg tggtacgaaa caggggaggg ggtctccagc accttcctct
      421 gccccgccgg aaaaaggtga gaggcatgct gaggaagacg atgctgctgg cgacggtcgt
      481 ggccctggtg ctgagcgttg ccgccacggc gctcgcctcg agtccgggcg acggcaaagc
      541 ccacgccagg gaaagcatgg acaagaacga actccgcaag aaagaggagt tcgccggcct
      601 ggtcgacatc ggcgggggcc ggcaggtgta catggagtgc cgcggtaaag gacgtcccac
      661 ggccgtgctc gtctccggcg gggcgggctc acatgaggac tggacccacc tgatagacgc
      721 cgaaggcggg ctagagccga gcggctctgc ggtgttcccc caggccggca agttcacccg
      781 ggtctgcgtc tacgatcgtc ccgggacctt gcgtttcgac ggcacgctgt ctccctcgac
      841 cccggtgcaa cagccgacca ccgccagcga cggtgccgcc gatctgcacg cgctgttgga
      901 ggcctccagg cagaaagatc ctcatgtcct ggtcgcgttc tcgtggggcg gcatgatcgc
      961 caggcaatat gttagcgatt atcccgacga ggtgtccggc ctcgtccttg tcgaccccgc
     1021 ctcggagttc ctgcaggaca ccctgacgcc ctcgcagtag gataccctgg tccggttggg
     1081 gacgcatccg cccgagggta tagaaggggt cgaccacggg tcaagcgcgc ctacactacg
     1141 cgcctccccc caggtgcgcg gcatcccctc cgtggtgctc tcctcagacg agtgcttcga
     1201 actcttgccc ggtgagaaca cgtgccaggc gtggcgggag gctcaggatc ttctggtcgc
     1261 gcaactcggc gccgagcaaa taacccgtac cgactgcagc caccccatcc agctgcagaa
     1321 ccccgagatc gtgatcgact cggttcgcca ggtggtagaa gccgcccgca agaagtcgag
     1381 cgcgctcggg tagaagaacc acgctcagtg cgtcaaggcc tcgaaccaag cacgaggagg
     1441 cagatagcac gagcaggcgt tacagagggc ggggcttccg gatgctccgc ccctggcccc
     1501 gcgcggggag gcataggatc tccgtccccc aggggacatg ccagggccct cttcctgtcg
     1561 gccaccgacc ccgaaacccc ccgaactaca cctttatgtg cattctgcag atatctacaa
     1621 aaagtttcta aattctttgt tccaaaggct ttacaaagtg tggcgagcga tgtatcatcc
     1681 tgtcagtcgg ttggtgcgaa actaaaccgg cacaattaaa gagcccgggg cgctactcct
     1741 cctttccctt tccccaccta tcacccctag gtgcgccccg ggttcttccc ctttctttag
     1801 ctcttagcgg tcagctttca gcttttttct ttcggtgcga ttctttgcgt gtaggcgcta
     1861 gtttgcgtga aattgcggcc tccgacgttc tcgtcggagg ccgcaagctg acggctgaaa
     1921 gctggccgct gatagctcta ggaagcttcc tggccttctg cctgggtcag gtagtagagg
     1981 gtgtgcatta gctgggccgt tgggtctata cggtggacgt ctacgtgggt tgggacgtga
     2041 agcaggacgt cggtgtagtt gaggatgacc ctgatgtcgg aatcgaccat gaggtcggcg
     2101 acttcctggg ccgaggaggc gggggtggcg atgatgccga ttatctcctc cgcttcggtg
     2161 acgctcttct tcagctcgtc gatgtgcttg accacgacgt tgcccacggt acgaccgatc
     2221 ttctccgggt tgtcgtcgaa gacgtcgtgg atcacgaagc ccctcttggg gaggatggtg
     2281 cttgccgcta tggcgcttcc caggttgcca gcgccgacga gggcgatgtt gtaggtctgc
     2341 ctcagaccaa ggatgtcgcg gacggcctcg accagatcgt aggcctggta gccgacccca
     2401 cgggtgccgg agaagcctat ggcgttcagg tcgcggcgta cctgcaccgg gtttatgccg
     2461 gtgaaatcgc ctagctcctt gctagagacc gcgtcacgac cctcttctat cagctgctgc
     2521 gtggtcctga gatacttcgt cagcctattc gtcaggccgg gctgtagcgt cgatcgcacc
     2581 ttggctcccg ctccttcttc gtgagtagat attcgatggg ggcattgtaa caaaatgggc
     2641 cgttcttgca ataaagatgc tcttaaccga caacaaatca gtaagcaagg ctaattacac
     2701 ttcggctgcc cgtgcccgcc accacgctcc ccggcaacgg ccggctatcg gcatcctcga
     2761 ctattcggct gcgggcgggt agacgtcagc tctctacgtt ccgtggcgtg taacataagc
     2821 gttcgacgtt ctcatgaggg aggttgcgat ggagtacagg aggctgggcg gtagcggggt
     2881 cagggtcagt gagatctcgc tcgggagctg gctcacttac ggggggagcg tcgccgagga
     2941 gcaggctacg gcctgcatcg accgtgcgta cgagatcggg atcaacttct tcgacacggc
     3001 caacgtgtac aggcgcgggg ccgcggagga gatcgtgggt cgggcgttga ggaagttcga
     3061 tcgtgactct tactttctgg cgaccaaggt gtactttccg atgggcgagg gacccaacga
     3121 cagggggctc tcgcgcaagc acataaccga gcagtgtcac gcctcgcttc ggcgccttgg
     3181 cgtgaattac gtcgacctct accagtgcca ccgctacgat gagaacacgc cgctggagga
     3241 gacgctgcgg gcgctcgacg acctcgtgcg gcagggcaag gtgctctacg tgggcgtctc
     3301 cgaatggacg gccgaccaga tctccgacgc cctcaggatc gcgggggaga tgaacctgga
     3361 ccggatcgtc tccaaccagc cgcagtacaa catgatccaa cgccgcatcg agccagacgt
     3421 gatacccctg tgtgaacgcg agggcgtagg gcaggtcgtg ttctcgccgc tcgcccaggg
     3481 cgtgcttacg ggcaaataca ggccgggtga cgcccccgag gagggtaccc gcgccgccga
     3541 cccggagaac aacgtgttca tgcagcagct catgaacgag cctgtcctct ccgcggtcga
     3601 cgggctgcgc tccgtcgcct ccgactccgg cctgagcgtg ccgcagctcg cgctggcgtg
     3661 ggtgctgcgc caggaggacg taagcagcgc catcatcggg gcatccaggc ccgagcaggt
     3721 cgatgacaac gccgcggcct ccggcgtgaa actctcagcg gacacgctct ccgagatcga
     3781 ccgtattctg gatggcgtta cccggttcga gtagacgctt
//
LOCUS       DBNGVD010000087         4738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1294580, whole genome shotgun sequence.
ACCESSION   DBNGVD010000087 DBNGVD010000000
VERSION     DBNGVD010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1294580"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="AC16CM_02645"
     CDS             <1..1007
                     /locus_tag="AC16CM_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HIR3138754.1"
                     /translation="CTGCSLCWDFCPRGGLQYESTWKITGNGESVEGIGRVEESYTAR
                     VKQKMDGVQDGGFVSALLVSLLKEGEIDGALVARESETERWKGEAFLARTPEEIRECA
                     GSFYNQTLALGHVDFKDYDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSSKVEAITL
                     TIALLCTKSFNYEKLMLEEIRDKRNVDLDNVGRVDIIRGKMIVQDLEGETIFGEPIRD
                     FHGAALKGCDECADFMGHAADISVGSVGSADGYSTVLVRSEEGLAAFDSVRDLLELRP
                     LDKPQALDKLDDLDKRVAFNSLKRAFDPDAPLFIDYEEHLNDYAGTDRAPVEHEAVRY
                     "
     gene            1035..1343
                     /locus_tag="AC16CM_02650"
     CDS             1035..1343
                     /locus_tag="AC16CM_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138755.1"
                     /translation="MPDEPEDLKTETRALLEESPEEGGRLVEDTTFVADLLWEEWRVD
                     LEGSGMGYDRFLQISRGYAAEFRLWVVGERPWNHCAAGLAGRLLRRLPAQEAAIPAEV
                     "
     gene            1346..2539
                     /gene="sat"
                     /locus_tag="AC16CM_02655"
     CDS             1346..2539
                     /gene="sat"
                     /locus_tag="AC16CM_02655"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR3138756.1"
                     /translation="MNVSTEFTTIEPHGGELVDRLAPEEDRDELLRRAEGLTKINLGP
                     RALSDLEMISTGVFSPLTGFMVSEDYESVVEKMRLKNGLVWSLPITLPVSEEEAEDIR
                     VGEEVALADGTGRVLATMIVEDRYSYDKEHEAQQVFGTTNDEHPGVAAVYRQKDVLLG
                     GEVTLLVEDPNPKPFPEYHYTPHELRQAFAEKGWKRVVGFQTRNPVHRSHEYIQKSAL
                     EITDGLLLNPLVGETRSADIPADVRMRSYEVILKKYYPKDRTMLAVFPAAMRYGGPRE
                     AIFHAICRKNYGCTHFIVGRDHAGPGKDSQGNPFYGDYDAQHIFDEFEDDDIGLTPLF
                     FEHAFFCLECQGMGTTKTCPHGSEHHVFISGTKVREMLSKGEYPPPEFSRPEVIEVLM
                     EGQRT"
     gene            2613..3185
                     /gene="cysC"
                     /locus_tag="AC16CM_02660"
     CDS             2613..3185
                     /gene="cysC"
                     /locus_tag="AC16CM_02660"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3138757.1"
                     /translation="MENGTRNGRGFTLWFTGLSGAGKTTIAEIVEHELRTRTRKIEVL
                     DGDIVRTNLSKGLGFSREDRDTNVLRIGFVADLLTRNGVGVIVSAISPFREVRDQVRQ
                     NVGEDFIEIFVDAPLEVCADRDVKGLYKKAFSGEIPQFTGVSDPYEAPETPELHIKTD
                     EEEPRESAARVIGHLEELGYLRPVGALNTA"
     gene            3198..4001
                     /locus_tag="AC16CM_02665"
     CDS             3198..4001
                     /locus_tag="AC16CM_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3138758.1"
                     /translation="MMIDPAPALFGLLVGFLVGLTGVGGGSLMTPFLVTVVGVPAPTA
                     VGTDLTFATLTKLTGSFQHFRQQTVNVELAVFLGMGSIPAGLLGVGTLEWIKGTFNPA
                     TVESIMVTIIAVTLVIVGVSLIFREYFMPKRSRPPRPGAWNGKGPMGTRRRVLTVLLG
                     AFGGYLVGLTSIGSGSVMAVLLLLFYPVAPAIIVGTDIAHATVLSFVVGIAHMTQGNV
                     NFGLAGTLLLGGIPGVLVGARLAPYIPAKPLKAILACMLIFVGLRLLLV"
     gene            complement(4030..>4738)
                     /locus_tag="AC16CM_02670"
     CDS             complement(4030..>4738)
                     /locus_tag="AC16CM_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138759.1"
                     /translation="EIGLMTELVEEGIAEATAVEDEEMLTFLLIQRGYAATFRGDLNA
                     GEEALSEALAVLRDHGGRWGTAPVLNALSQVALSRGDFVKAIGFLREGEAVSRETEDA
                     FTLATNLNIQATISQLEGDEERTRTVLEESVALSSALRDSWALVYGLVGLAGIAARHG
                     EPERAARLFGAAEAMGEAASVTIAFPPARALYEQDLSTVKGRLDADAFEASWERGRSM
                     GVKEAVAEALPGNPTDP"
BASE COUNT          896 a         1475 c         1527 g          840 t
ORIGIN      
        1 tgtgcacggg ctgctcgctg tgctgggatt tctgcccgcg tggcgggttg cagtacgagt
       61 cgacgtggaa aatcaccggt aacggggaaa gcgtcgaggg catagggcgc gtcgaggaga
      121 gctacacagc gcgggtaaag cagaagatgg acggtgtgca agacggcggg ttcgtcagcg
      181 cgctcctcgt ctccctgctc aaggaaggtg agatcgacgg cgccctcgtt gctcgggaga
      241 gcgagaccga gcgctggaag ggcgaggcct tcctggcgag gacgccggag gagatccgcg
      301 agtgcgcggg cagcttctac aaccagacgc tcgccctggg acacgtggat ttcaaggact
      361 acgacctgcc cccaaacccg aggatcgccg tcgttggaac gccctgcgag atcgagggca
      421 tcaaggccat gcaggcgagg ccatggacgt ggggctcatc caaggtcgag gccataacgc
      481 tcaccatagc gctcttgtgc accaagagct tcaactacga gaagctgatg ttggaggaga
      541 tcagggacaa gaggaacgtc gacctcgaca acgtgggccg cgtggacatc atccgcggga
      601 agatgatcgt gcaggacctc gagggcgaga ccatcttcgg ggagcccata agggacttcc
      661 acggcgcggc cttgaaggga tgcgacgagt gtgccgactt tatgggccac gccgccgaca
      721 tctcagtggg cagcgtgggg agcgcggacg gctactccac cgtcctggtc aggagcgaag
      781 aggggctcgc cgccttcgat tccgtcaggg accttctgga gttgcgtccc cttgacaagc
      841 cgcaggcttt ggataagctt gacgacttgg acaagcgggt ggctttcaac agcttgaaac
      901 gggccttcga ccccgacgcc ccactcttta tagactacga ggaacacctg aacgactacg
      961 ccgggacgga tcgggcgcca gtcgaacacg aagcggtgag gtactagttg aaacgtgcca
     1021 caacgagtct ctctgtgcca gatgagccgg aagacctgaa gaccgagacc cgcgcgctcc
     1081 ttgaggaatc gcccgaggag ggtggccgcc tcgtcgagga caccaccttc gtggccgact
     1141 tgctctggga agaatggcgg gtcgacctcg aagggtccgg catgggttac gaccggtttc
     1201 tacagatctc acgcggctac gccgccgagt tcaggctctg ggtggtcgga gaacgtccct
     1261 ggaatcactg cgcggccggc ctcgccggcc gcttgttgcg aaggctcccg gcccaagagg
     1321 ccgcaattcc cgcggaggta taaccatgaa cgtcagcacc gagtttacga ccatcgagcc
     1381 gcatggcggt gagctggtag accgtctggc tcccgaagag gaccgtgacg agctactcag
     1441 gagggccgag gggctcacga aaataaacct cggcccacgc gccctctcgg acctggagat
     1501 gatctcgacc ggggtcttct cgcccctgac cggctttatg gtcagcgagg actacgagag
     1561 cgtggtcgag aagatgcgcc tcaagaacgg cctggtctgg agcctgccca taacgctccc
     1621 cgtgagcgag gaagaggcgg aagatatcag ggtcggcgaa gaggtcgccc tggccgacgg
     1681 cacgggacgc gtgctggcga ccatgatcgt ggaggaccgc tactcctacg acaaggagca
     1741 cgaggcccag caggtcttcg gcaccaccaa cgatgagcat cctggcgtcg ccgccgtata
     1801 ccgccagaag gacgtgcttc tcggcggcga ggtgacgctc ctggtggaag acccgaaccc
     1861 caagcctttc cccgagtacc attacacccc gcacgagctg cgacaggcgt tcgcggagaa
     1921 gggttggaag cgcgtggtcg gcttccagac ccgtaacccc gtccaccgct cgcacgagta
     1981 catccagaag agcgcgctcg agattacgga cggcctgctt ctgaacccgc tagtcggtga
     2041 gacccgctcc gcggacatcc cggccgacgt caggatgagg tcctacgagg tgatcctgaa
     2101 gaagtactac ccgaaggacc gcacgatgct cgccgtgttc cccgctgcga tgcgctacgg
     2161 tgggccgcgt gaggccatct tccacgccat ctgccgcaag aactacggct gtacgcactt
     2221 tatcgttggc cgcgaccacg caggccccgg caaggactcc cagggcaacc cattctacgg
     2281 cgactacgac gcccagcaca tcttcgacga gttcgaagac gatgacatcg ggcttacccc
     2341 cctgttcttc gagcacgcct tcttctgcct ggagtgccag ggcatgggca ccaccaagac
     2401 ctgcccccac ggcagcgagc accacgtctt catcagcggc accaaggtgc gcgagatgct
     2461 ctccaaaggc gagtacccgc cgccggagtt ctcccgcccc gaggtgatag aggtgcttat
     2521 ggaaggtcag cgcacgtagc aaaattgttt atttacgtta aagtaagtta aactagtaac
     2581 agtacgtaac tggtaggcaa ggagatctaa cgatggagaa tgggacgagg aatggtcgcg
     2641 ggttcaccct gtggttcacg gggctatctg gcgcgggcaa gaccacgatc gcggagatcg
     2701 tagagcatga gttgaggacc cgcacccgca agatagaggt tctggacggg gacatcgtga
     2761 ggaccaacct gtccaagggg cttggcttct cccgcgagga cagggacacc aacgtcttga
     2821 ggatcgggtt tgtggccgac ctgttgacgc gaaacggggt aggggtgatc gtcagcgcca
     2881 tctcgccctt cagggaggtg cgcgaccagg tcaggcagaa cgtgggcgag gacttcatcg
     2941 agatcttcgt cgacgcgccg ctagaggtat gcgccgatcg tgacgtcaag ggcctctaca
     3001 agaaggcttt ctcgggggag atcccgcagt tcactggcgt ctccgacccc tacgaggcgc
     3061 ccgagacccc cgagttgcac atcaagaccg acgaggagga gccgagggag agcgcggcaa
     3121 gggttatcgg gcacctcgaa gagcttgggt acctcaggcc ggtcggggcg ctcaacactg
     3181 cctaggaaca ccgacgcatg atgatagatc ccgctcccgc gctattcggt ctgctggtcg
     3241 gcttccttgt cggcctcacc ggcgtcgggg gcggttcgct tatgacgccc ttcctggtga
     3301 ccgttgtggg ggtgccggcc ccgaccgccg tcggaaccga cctgaccttc gccacgctga
     3361 ccaagctgac cggctccttt cagcattttc ggcagcagac ggtgaacgtg gagttggccg
     3421 tctttctggg gatgggaagc atccccgcag ggctcctcgg ggttggcacc ctggagtgga
     3481 tcaagggcac cttcaaccca gccacggtgg agtcgatcat ggtgacgatc atagccgtta
     3541 ccctggtcat cgtgggggtc tcactgatct tccgcgagta cttcatgccc aagaggtcga
     3601 ggccaccgag gcccggggcg tggaacggta agggaccgat gggcacgcga cgccgcgtgc
     3661 ttaccgtgtt gctcggggct ttcgggggct acctggtggg gctgacgtcc atcggctcag
     3721 gaagcgtgat ggccgtactg ctgctcctct tctatcctgt agcacctgcc atcatcgtgg
     3781 gcacggacat agcacacgcc accgtgctct ccttcgtggt cggcatagcc catatgacgc
     3841 agggaaacgt caacttcggc ctggccggga ccctgcttct cggcggcatc cccggcgtgc
     3901 tggtcggggc caggctcgcg ccctacattc cggccaagcc cctcaaggcg atcctggcct
     3961 gcatgctgat cttcgtgggc ttgcgtctgt tgttggtgta gcgttcagcg ggcgcttcac
     4021 gaagcgcccc tacgggtctg tggggttccc ggggagtgcc tctgcaaccg cctccttcac
     4081 gcccatcgaa cggcccctct cccaggacgc ttcgaaagcg tcggcgtcta gccttccctt
     4141 cacggtcgag aggtcttgct cgtagagggc cctcgcgggg ggaaacgcta tcgttactga
     4201 ggccgcctcg cccatggctt ctgcggcccc gaagaggcgg gccgcccgtt cgggttcgcc
     4261 gtgtctagcg gctattcctg ccaggccgac caggccgtag accagcgccc agctatctcg
     4321 cagggcggat gaaagggcta cgctctcttc gagcacggtc ctcgtgcgct cctcgtcacc
     4381 ttcgagctgt gagatcgtgg cctggatgtt caggttcgtg gccagcgtga aagcgtcctc
     4441 tgtctcgcgc gacacggctt ctccttcacg caagaatcca atcgccttta cgaagtcgcc
     4501 gcgcgagagc gccacctgag agagcgcgtt cagcaccggg gcggtgcccc agcgtccgcc
     4561 atggtctcgc aacaccgcca acgcctcgga gagggcctcc tcgccggcgt tcaggtctcc
     4621 ccggaacgtg gccgcgtagc ccctctggat gagcagaaag gtcagcatct cctcgtcctc
     4681 caccgcggtg gcctccgcga tgccctcctc cacgagctcg gtcatcagcc cgatctca
//
LOCUS       DBNGVD010000088         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4026954, whole genome shotgun sequence.
ACCESSION   DBNGVD010000088 DBNGVD010000000
VERSION     DBNGVD010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4026954"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC16CM_02675"
     CDS             <1..489
                     /locus_tag="AC16CM_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR3138760.1"
                     /translation="ETVFVDSSTTAYYAARRMLAGATGFTCLTNLIPVMDLFSTADPS
                     GASMVGLGGIFRPLTLSFVGPCAIRAAESYVADRAFFSVKGITSGGHLTEPNPLEAEV
                     KRAMILRSEEPVLLVDGRKFERRGASVIAHVSEVSLVITADAQARHVEHLEDLGVEVR
                     SV"
     gene            578..1951
                     /locus_tag="AC16CM_02680"
     CDS             578..1951
                     /locus_tag="AC16CM_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138761.1"
                     /translation="MASSEADVNGQHDDGHITAQQWKWGVLAGMASYLDAGSIVALAA
                     GLALWQEYLNMSSTTVGLLAALGPNAFGAALGALVGGRLGDLFGRKRIYQYDLLVYAL
                     GTLLIIFSFNLPMLLVGTFIVGVAVGADVPTSLALIGEFSPSKGRGKLMGLSQVAWSL
                     GPVVVYLLAFALSSYGVLGNRIVFASLFVVAIVTWALRRGMVESAIWTAASGAGEAES
                     TTAPEEQPEEAGVPADPLAASRLRGLLSGPTMAAMVFTAVVYLFWNLAAGTFGVFQPD
                     ILKTLGTQSQAVSVGLSCAGFVLTLLATVFIFMPFSDRSDRARRTMWAIGAVLNVASV
                     AQLLIFPFNTVTAVMLVVLFGIGAGIAGEAFYKTWSQELFPTMLRTTAQGLTFSVARM
                     LLGIWSFFVPVLASLSIRPVALILMIFLFISGVVGYLFMPSTTGKSLEEIESERSGDP
                     TRERVPA"
     gene            1948..>3859
                     /locus_tag="AC16CM_02685"
     CDS             1948..>3859
                     /locus_tag="AC16CM_02685"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR3138762.1"
                     /translation="MTVSSSSLGPVGLKCEYRTDPLGIDALVPRLSWALESEERGQVQ
                     SAYRILVARGEEDLDSEENLLWDSGRVESDRSVGIEYGGEVLRSGSRCVWKVRVWDGE
                     DEPTPYAEPAVFEMGLLERSDWEGAWISRGEDPDEAFEPPSGDEYDDLANGLAPSPYL
                     RREFSLGGPVRRARLYATARGLYEASINGTRVGDDVLAPGWTDYDRRIQYQTYDVTRL
                     LTEGQNVLGAVLGDGWYAGFLGFDPKQRGALYGAHPQLLAQLKVEYEDGTTETIATDR
                     SWRCSTGPILYSDLLMGESYSAREEMPGWSAPGFDDAAWYGVDAGELGDANLVAQPDE
                     GVKVMQELETRSVTEPESGRYVFDLGKNMVGWVRLRVEGEAGTEITLRHAETLNPDGT
                     IYTTNLRSARATDRYILKGGGEEIYEPRFTFHGFRYVEVTGYPGEPRPETITGCVVHS
                     ATPSAGSFECSNGMVNELQENILWGQRGNFLSVPTDCPQRDERLGWMGDAQVFVRTAS
                     LNMDVAAFFEKWMADVEDAQSPEGAFPDVAPLLKGSDLIDLRWGAPAWGDAGVIVPWT
                     IYRTYEDTRIVERHYDAMARWMGYLHEANPDLIRRNRMGNNYGDWLSPKGDHTPKHLL
                     ATAYWAYDAGLMA"
BASE COUNT          644 a         1225 c         1290 g          700 t
ORIGIN      
        1 gagaccgtat tcgtcgactc ctcgaccacg gcctactacg cggcgcggcg catgctggcc
       61 ggtgccacgg gttttacctg cctcaccaac ctgatacccg tcatggacct cttttcgacg
      121 gcggatcctt cgggagccag catggtcggg ctcggtggca tcttcagacc attgacgctc
      181 tccttcgtcg ggccttgtgc catacgcgcg gccgagtcct acgtggcaga cagggccttc
      241 ttcagcgtca aggggatcac gtcggggggg catctcacgg agccaaaccc gctggaggcc
      301 gaggtcaagc gggccatgat ccttcgttcc gaggagcctg tgttgttggt ggacgggaga
      361 aagttcgagc ggcggggggc gagcgtgatc gcccacgtct ccgaggtgtc gctcgtgata
      421 accgccgacg cccaggcccg gcatgtagag cacctcgaag atcttggggt ggaggtgcgg
      481 agcgtgtgag gggagtaggc cgaggaccgg ggtagcgttg cggcggttgt gggcagtcct
      541 ggagggtatc aacgaggggg gaggctctac ctagatcatg gcaagttcgg aagcagacgt
      601 caacggacag cacgacgacg gacatatcac agcgcagcag tggaagtggg gtgtactggc
      661 tggcatggcg tcctatctgg acgccgggtc catagtggcg ctagccgccg ggctcgcgtt
      721 gtggcaggaa tacctcaaca tgagcagcac gaccgtgggg ttgctcgcgg ccctcggtcc
      781 caacgccttc ggggcggccc tcggggccct cgtgggtggc aggctcggcg acctgttcgg
      841 ccgtaaacgc atctaccagt acgacctgct cgtctacgct cttggcacgc tgctcataat
      901 cttctcgttc aacctgccga tgctcctcgt aggcaccttc atagtaggcg tcgcggtggg
      961 tgctgacgtg ccgacctcgc ttgcgctcat cggcgagttc tccccgagca aaggccgcgg
     1021 caagctcatg ggtctcagcc aggtggcatg gagcctgggt cccgtggtgg tatacttgct
     1081 cgcgttcgcg ctctcctcgt atggggtact cggcaacagg atcgtctttg ccagcctgtt
     1141 cgtggtcgcg atagtcacgt gggctctcag gcgtggcatg gtcgagtcgg ccatctggac
     1201 agccgcgtct ggcgcgggcg aggcggagag tacgaccgcc cctgaagagc agcccgagga
     1261 ggccggtgtt ccggccgacc cgctggccgc cagccgtctg cgcgggttgc tctcgggtcc
     1321 gacgatggcc gcgatggtct tcaccgcggt cgtttacctt ttctggaacc ttgcggccgg
     1381 tactttcggc gtcttccagc ccgacatact caagaccctt gggacgcaga gccaggcggt
     1441 aagcgtcggc ctctcgtgtg cgggcttcgt gctcacgctg ctcgccaccg tctttatctt
     1501 tatgcccttc agcgaccgga gcgaccgcgc ccgccgcacg atgtgggcca tcggggccgt
     1561 attgaacgtc gcctcggtcg cacaattgct catcttcccc ttcaatacgg tgacggccgt
     1621 catgctggtc gtcctcttcg gcatcggggc tgggatcgct ggcgaagcct tctacaagac
     1681 gtggtcgcag gagttgttcc caaccatgct caggaccacg gcccaggggc tcacgttcag
     1741 cgtcgcccgc atgcttctcg gcatctggag cttcttcgtg cccgtgctcg cgagcctcag
     1801 catcaggccg gtagccctga tactcatgat cttcctcttc atcagcggcg tcgtcggcta
     1861 cctgttcatg ccgagcacca ccggcaagtc cctggaggag atcgagtccg agcgtagcgg
     1921 agatccaact cgcgagagag tcccggcatg acggtgtctt cgtcctcgct ggggcccgtg
     1981 ggcctcaagt gcgagtaccg caccgatccg ttgggcatcg acgcgctggt gccgaggctg
     2041 agttgggccc tcgagtcgga ggagaggggg caggtgcagt cggcctaccg catcctggtc
     2101 gcgcggggcg aggaggacct cgattccgag gagaacctgc tctgggacag cggcagggtg
     2161 gagtcggacc gctccgttgg catcgagtac gggggagagg tactacggtc tggctcgcgc
     2221 tgcgtgtgga aggtgcgtgt ctgggacggt gaggatgagc cgacgcctta tgcggaaccg
     2281 gccgtcttcg agatggggct tctagagagg tcggactggg agggggcctg gatctcccgc
     2341 ggcgaagacc ccgatgaggc tttcgagcca ccgtcaggag acgaatacga cgacctcgcc
     2401 aacggcctag ccccgagccc ttacctgcgc cgggagtttt ctctaggcgg tcccgtccgc
     2461 cgggcgcggc tctacgctac ggcccgaggc ctctacgagg cctcgatcaa cggcacgagg
     2521 gtgggcgacg atgtgctggc ccccggctgg actgactacg acaggcgcat ccagtaccag
     2581 acctacgacg tcacgaggct gctcacggag gggcagaacg tgctcggcgc cgtgctgggc
     2641 gacggatggt acgccgggtt cctcgggttc gaccccaagc aaaggggggc gctctacggt
     2701 gctcacccgc agcttctcgc ccagctcaag gtcgagtacg aggatggcac cacggagacc
     2761 atcgcgaccg accgctcctg gcggtgctcc acgggcccta tcctctactc cgacctcctg
     2821 atgggagagt cctacagcgc cagggaggag atgccaggct ggtcggcccc aggcttcgac
     2881 gacgcggcgt ggtacggggt agatgccggg gagctggggg acgccaacct cgtcgcccag
     2941 cctgacgagg gcgtaaaggt catgcaggag ctggagacac ggtccgttac cgagccggag
     3001 agtggccgct acgtcttcga cctcggcaag aacatggtcg gttgggtacg cctcagggtc
     3061 gagggggagg ccgggaccga aatcaccctg cgccatgccg agaccctgaa cccggacggc
     3121 accatctaca cgaccaacct ccgctctgcc agggcgaccg accgctacat cctcaaaggt
     3181 gggggcgaag agatctacga gccgcgcttc accttccatg gcttccgcta cgtcgaggtt
     3241 acgggctatc ccggcgagcc ccggcccgag accataacag gatgcgtcgt ccactcggcc
     3301 acgccatctg ccggctcttt cgagtgttcg aacgggatgg tcaacgagct acaggagaac
     3361 atcctctggg gccagcgggg caacttcctt agcgttccta ccgactgccc gcagcgcgac
     3421 gagcgcctcg ggtggatggg cgacgcacag gtcttcgtcc gcaccgcctc cctcaacatg
     3481 gacgtggccg ccttcttcga gaagtggatg gccgacgtcg aggatgccca gtcccccgaa
     3541 ggcgccttcc cagacgtggc ccccctcttg aagggttccg acctcataga cctcaggtgg
     3601 ggggctccag cctgggggga tgccggcgtt atcgtgccgt ggaccatcta caggacctac
     3661 gaagatacgc ggatcgtcga gcgtcactac gacgccatgg cccgctggat ggggtacctg
     3721 cacgaggcca accccgacct catccgcagg aaccggatgg gcaacaacta cggcgactgg
     3781 ctctcaccaa aaggggacca cacccccaaa catctcctcg ctacggccta ctgggcctac
     3841 gacgcggggc ttatggccg
//
LOCUS       DBNGVD010000089        12993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1840637, whole genome shotgun sequence.
ACCESSION   DBNGVD010000089 DBNGVD010000000
VERSION     DBNGVD010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1840637"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16CM_02690"
     CDS             complement(<1..283)
                     /locus_tag="AC16CM_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HIR3138763.1"
                     /translation="MVDSRVAGITERGWPEPGSAEELTIFAALQERLEPLAQKVISDP
                     RASQTVVVVPSLTMDTDELAKIPGAHHYEERMLCMLMLLRLPRTHVIYVT"
     gene            complement(448..1575)
                     /locus_tag="AC16CM_02695"
     CDS             complement(448..1575)
                     /locus_tag="AC16CM_02695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138764.1"
                     /translation="MRIGQYSSIKHVGEAAWRALEPPDFPFFDYEFLLALERSRSVGR
                     RTGWEPVYLTCEDGGRVLGAMPLYLKTDSYGEYVFDWAWANAYHQNGLSYYPKLVSAV
                     PFTPATGPKLLVSPDADRAIVRRALLDASLELGEDQGVSSSHALFLPEVELDEFQDHG
                     FAVRHSLQFHWRNRGYGVFDDYLEAMTSKRRRQVARERRQLDGVEVEYLTGDGLGPEH
                     AAMMYGYYVDTYDRKWGSPYLTGAFFDEVFRTMKDRIMLAVAREETTGRPVAGALFFF
                     KGSALFGRYWGATRAVRNLHFELCYYQGIEFAIDRGLSLFEAGAQGEHKLARGFLPAV
                     TYSAHEIRHPAFRRAIENYIEEERDMISYELAEYMDHDPYK"
     gene            complement(1758..2648)
                     /locus_tag="AC16CM_02700"
     CDS             complement(1758..2648)
                     /locus_tag="AC16CM_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3138765.1"
                     /translation="MRMISPEAAKVYEIFRNMPKQVDMDLHHQREAGEHAEDMTSEPE
                     GVEYEAVPELGGFWATPADWDGESALVYLFGGGYVISSPHSRRKLAGHLASASGARFL
                     VPKYRLAPEQPFPAAVEDAAAAFRWLLDRDFRPERVLIGGDSSGGGLTVATLLKLRND
                     GGPVPGGGVMISAWVDLACTGKSLDTRADVDLTVTRSSLERMAGQYLDGVDPRTPLAS
                     PLYGELSGLPPLLMTVGGDEALLDDSVRFLRAAAVAEVDVTLSVGANMQHIYPIYCGA
                     MPEADAAVALIGNWIRTRLA"
     gene            2885..4666
                     /gene="typA"
                     /locus_tag="AC16CM_02705"
     CDS             2885..4666
                     /gene="typA"
                     /locus_tag="AC16CM_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3138766.1"
                     /translation="MEYRNIAIISHVDHGKTTLVDGLLRQTLDLGHGQQISDRAMDSN
                     VLERERGITILAKNTAVEYEGVKINIVDTPGHTDFGGEVERVLGMVDGCLLLVDAAEG
                     PMPQTRFVLRKAIELGLKPIVVVNKVDRADARPDEVVNLTFDLMADLGATDEQLDFPI
                     LYAVAREGRAFRDLDEPRDDMRELFETVLEEIPAPEVDLTAPFQMLISNLDYSEYLGR
                     IVVGRVRRGEVRRGESINLIHTDGTMTRTRVVQPFTHLGLERIEADEVGAGDIVALSG
                     IENAQIGETLADEAQPEALPAISVDEPTVSMTFGPSTSPFAGKEGRFVTSRHLRDRLR
                     REVQTNVSLRVEELRPDEFEVSGRGELHLSILLETMRREGYEVQVGAPRVIVREIEGK
                     KYEPFERLVIDVPERFASTVIGVISGRKGQLVDVEPQGSRTRVEFEIPARALFGFRTQ
                     FLSMTQGEGIMSHVFDGYAPWSGDLKTRRNGSLVASEAGSAFAYSIWKLQDRGSFFIK
                     PSTEVYVGMVVGENNRENDLNVNVCKNKKLTNVRSAGADDALNLTPIRKLTLEDALEY
                     IGEDELVEITPKNIRLRKKILEPGMRK"
     gene            4762..5688
                     /locus_tag="AC16CM_02710"
     CDS             4762..5688
                     /locus_tag="AC16CM_02710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3138767.1"
                     /translation="MTLHPALRELIYEKLANTTAPQWRLPIGQVRSSFRAFWSPAVTG
                     APVEVASVENRTVDTEAGAVPVRVFTPEADSRVPIMMYFHGGGYVKGGLDETDVFCRR
                     LARTTGYIVVSVEYRLSPEHRFPAALDDAYACSLWAYQNAEELGGTSDSFSVSGESAG
                     GNLAAVTCLIARADEKIEIRRQILLQPVVDFTLSFPSIGMPATECLVPRDDLAWYYGE
                     YYSGDTRDFRVSPIFAEDLSSLPPALIITAEHDTLRDEGEAYADRLQVAGVPTQYSCY
                     SGMIHGFLLLTGLVDEAQQAIDEIAAFTKAKI"
     gene            complement(5706..6317)
                     /locus_tag="AC16CM_02715"
     CDS             complement(5706..6317)
                     /locus_tag="AC16CM_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025160.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HIR3138768.1"
                     /translation="MGRLYRRSMGWLLREWPYAGFLAGLLLLALAPLWVSGMGLVIGV
                     IYFQLPVYMLHQLEEHAGDRFRRFVNDRVADGRDALTPEATLVINVVGVWMVNVIALY
                     LAMYVSPALGLISVYLTLVNAVVHVVGTVVLRAYNPGLWTAIILFLPLGIYSLFMLNS
                     AGAGVWANVLGILVAVAIHAAILVHIRHRIQALSTSSTNTRVS"
     gene            6533..7270
                     /locus_tag="AC16CM_02720"
     CDS             6533..7270
                     /locus_tag="AC16CM_02720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014949651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3138769.1"
                     /translation="MDGIEGKVVVVTGSSSGLGEATAKLLAERGARIMLGARREENLQ
                     RIASEIRDNGGEASYRVTDVTDRAQVENLVSGAVDEFGRIDVLVNNAGLMPLSPLDAL
                     KVDEWERMVDVNIKGVLYGIAATLPRMQEQHEGQIINLSSVAGHVVPPNSAVYSGTKF
                     AVWAISEGLRQEVGEKIRTTTISPGAVQSELTDHITHEETAEGTSELDEMAVDASAVA
                     RAIAYAIEQPRDVDTNEIILRPTAQAL"
     gene            7362..8582
                     /locus_tag="AC16CM_02725"
     CDS             7362..8582
                     /locus_tag="AC16CM_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138770.1"
                     /translation="MTGSTSAEVRQQENPTGSSDVSTLLVGLIALCAGVTVSNLYLTQ
                     PLLPLVAESLRVGPSQVGFLPTIGQAGYALGILLIVPLGDVLRRKGLLTFVLVGLIVA
                     LAGAALAPSLGFLMAATLFVSSLTILPQILVPLASQIAGDEHRGRVLAIIGAMMTCGL
                     FASRSLYGQVGDAFGWRASYVVAAVLSAVVGAVTIFVLPKEKGKEGETYASLLRSLST
                     LLVEEPTLRKACLLQGTIFATFNMFWTTIVFTLTSSPYGFSTGTAGLFGLFGILSATS
                     APFVGRLIDRRGPIPTLGACLLVTTCSAAAFLLGGTTILALILGIVLLGVGMQGGMSA
                     NQTRVLTIRPEAGSRMNTVFLVLIFVCGAAGGAAGSALYQAGGWPLVCISGIGVSTAG
                     FIAWLILDYRGFRN"
     gene            complement(8625..9242)
                     /locus_tag="AC16CM_02730"
     CDS             complement(8625..9242)
                     /locus_tag="AC16CM_02730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138771.1"
                     /translation="MRGTDDSSMGGLRPPLRRATRRDARAIAELSDQAGHGIPGYLWS
                     QSAKEGQSPVEAGIERALREDANFSYRNAVVAEVNGRVAALMLAYPLPEHSDVNLDEV
                     PDLVRPLEELEQRVPGTFYINIIATYPEYRGTGLGTKLLEAAPALASGAGCSELSLQV
                     FEQNEGALRLYERHGFREVARLPTVPHPSYPYEGYVVLMTRSVAP"
     gene            complement(9389..9511)
                     /locus_tag="AC16CM_02735"
     CDS             complement(9389..9511)
                     /locus_tag="AC16CM_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138772.1"
                     /translation="MTIIVLTNLYAAPDGNTPATVIPKLIIGELADTEGESQTK"
     gene            complement(9907..10248)
                     /locus_tag="AC16CM_02740"
     CDS             complement(9907..10248)
                     /locus_tag="AC16CM_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3138773.1"
                     /translation="MIEASCHCGAVRLEIDAPLSEVAECNCSICRRYGVLWAYYALDQ
                     VRLLPSDLPTNIYMWNDRMIEFHHCRTCGCISHWFPVDREFNRMGVNARLMDPEILAS
                     AHVRHIDGARD"
     gene            10521..11636
                     /locus_tag="AC16CM_02745"
     CDS             10521..11636
                     /locus_tag="AC16CM_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3138774.1"
                     /translation="MESLTWDQVNAWRLARHGLAPRVGFLEAARRMIGVQAQVMSAAE
                     LSLWARVDGLRPSDVQTALWQDRTLVKTWAMRGTLFLISAEDLPLVVATRRIRKARYW
                     VKHFESYGIHEPEYEALLDAIPQVLGDEPMTREQLAVAAAEHTGIDGLLPALAESSWG
                     TALKPSAFRGDLCYGPTLGRNVAFVRPSEWLDGWREIEPEVAARRIVRRYLSVYGPAT
                     PQDFGRWWGAGTRLATKTFREFGDELEAVDIEGHRAFALRSSLEPMESLSAAGVVRLL
                     PMFDAYTHGSIDYEQLLPTEFRRLIFRPQGWISAVVLVDGRIVGVWDYMTGSSVTTVK
                     VRMFATPDARTRDAIVAEAERLNDFLDTRATVEFDSA"
     gene            complement(11800..12198)
                     /locus_tag="AC16CM_02750"
     CDS             complement(11800..12198)
                     /locus_tag="AC16CM_02750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR3138775.1"
                     /translation="MELPEALISLLRQPSTCYIATTMPDGSPQLTQTWVDTDGEHILI
                     NTVQGYQKVLNVGRDPRVAVTVSDPDNPLRYYSVRGHVVDITANGGAEHIEMLSQRYL
                     GAPYQWSRGGGRDQVRLILTVSADRIHSPW"
     gene            complement(12373..>12993)
                     /locus_tag="AC16CM_02755"
     CDS             complement(12373..>12993)
                     /locus_tag="AC16CM_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3138776.1"
                     /translation="GGPMVGGLHEHLGRRLRMAMAARRGFGGHGPGGHGPAGFPFGGF
                     RGGGFGHRPRRMGQGDVRGAVLVLLSERPMHGYQIIQEIGERSGGVWNPSPGSVYPAL
                     QQLEDEGLVRVEKADGRKVAHLTDEGDTYVREHRDKLGTPWKSAAEDYGEDLLELRNL
                     VGQVATATMQVAQAGDSAQISEARRLLTEVRRQLYRVLAGDDTKSV"
BASE COUNT         2369 a         4185 c         4112 g         2327 t
ORIGIN      
        1 tcgtcacgta gatcacgtgc gtccgcggca ggcgcaggag catgagcatg cacagcatcc
       61 gctcctcgta gtggtgcgcc ccggggatct tcgccagctc gtcggtgtcc atggtgaggc
      121 tcggtaccac cactaccgtc tgcgaggcgc gggggtccga gatcaccttc tgcgccaggg
      181 gctcgagcct ctcctgcaaa gccgcgaaga tcgtcagctc ctcggccgaa ccgggctcgg
      241 gccagccgcg ctcggttatc ccggcaacgc gagagtcaac catctctacc ccctacgcgg
      301 tgattacgag gtccagaagg acctccggta cactcctcca tgcgcgatct ttcttccgga
      361 ttctacgcgc tacatgccct ctcccatgtt aaccgcaagg cggattgcgt tacaagattc
      421 ctgtgtagtg cacccggagt ttctccatta cttgtacggg tcgtggtcca tgtactcggc
      481 cagctcgtag gagatcatgt cccgttcctc ttcgatgtag ttttcgatgg cgcgcctgaa
      541 ggcagggtga cggatctcgt gcgcgctgta ggttacggcc ggcaggaagc ccctggccag
      601 cttgtgctct ccctgcgccc cggcttcgaa gagcgagagc ccgcggtcga tggcgaactc
      661 tatcccctgg tagtagcaga gctcgaaatg caggtttctt accgcccttg tcgctcccca
      721 gtagcgcccg aagagggccg accccttgaa gaagaacaaa gcccccgcca cgggacggcc
      781 cgtcgtctcc tcccgcgcga cggcgagcat gatgcggtcc ttcatggtcc ggaacacctc
      841 gtcgaagaag gcgccggtga ggtaggggct gccccacttc cggtcgtacg tatccacgta
      901 gtatccgtac atcatggccg cgtgctcggg acctagcccg tccccggtga ggtactccac
      961 ctccacgcca tcgagctgcc gacgctcgcg agcgacctgg cggcgacgct tgctcgtcat
     1021 ggcctccagg taatcgtcga acacaccgta gccgcggttg cgccagtgga attgcaagga
     1081 gtgcctgacc gcgaacccgt ggtcctggaa ctcatccagc tcgacctccg gcaagaagag
     1141 cgcgtgcgaa gagctgacgc cctgatcctc gccgagctcg agggaagcgt cgagcagggc
     1201 acgtcttacg atggcccggt cggcgtcggg gctcaccagg agcttgggtc cggtggccgg
     1261 ggtgaacggc acggcggaga ccagcttcgg gtagtaggag aggccgttct ggtggtaggc
     1321 gttggcccag gcccagtcga acacgtactc gccgtaggag tcggtcttga ggtagagggg
     1381 catcgcgccg agaacgcggc ccccgtcctc gcaggtcagg tataccggct cccatcccgt
     1441 cctgcgcccg acgctacgcg accgctccag cgcgagcagg aactcgtagt cgaagaaagg
     1501 aaaatccggg ggctcgaggg cccgccatgc cgcctcaccc acatgcttta tgctggagta
     1561 ttgccctatt cgcatcgcgc taggccgaca cgattcgacg cgcggcggtt ataagcccga
     1621 gatcaggtac cacggcaacc atcgacccga gtttaccgga agattggcga ccgcgaggaa
     1681 agtagaggtc aacctgacat tagatgggtc tcccgttacg ttgaccaact ccgggtgagg
     1741 cttcggtcga gtcgacccta cgccaggcgg gtgcggatcc agtttcctat cagcgctact
     1801 gcggcgtccg cttctggcat ggcgccgcag tagatcggat agatgtgctg catgttggcc
     1861 ccgaccgaca gcgtcacgtc gacctcggcc accgccgccg cccgcaggaa cctcacagag
     1921 tcgtcgagca gcgcctcgtc acccccgacg gtcatcagca gaggcggaag cccagatagt
     1981 tcgccgtaga gcggggaggc gagcggggtg cggggatcca ccccgtcgag gtactggccg
     2041 gccatgcgct cgaggctgga cctggtgacg gtgaggtcga cgtcggcgcg cgtgtcgagg
     2101 cttttgcccg tgcaggccag atctacccac gctgagatca tcaccccacc gccggggacc
     2161 gggcctccat cgtttcgtag cttgagcagg gttgccaccg tcagcccgcc gccgctcgaa
     2221 tcaccaccga taaggacccg ctccggccga aaatcccgat ccagcaacca gcggaaggct
     2281 gccgccgcgt cctcgacagc cgcaggaaaa ggctgctcgg gagcgagtcg atacttcggg
     2341 accagaaagc gcgcccccga cgcgctcgcc aggtgtccgg ctagcttgcg gcgcgagtgt
     2401 ggggaggaga tcacgtaccc tccaccgaac aggtacacga gcgccgactc tccgtcccag
     2461 tccgcgggcg ttgcccagaa tccgcctagc tcggggacgg cttcgtattc gaccccctcc
     2521 ggttccgagg tcatgtcttc ggcgtgctcg ccggcctccc gctgatggtg caggtccata
     2581 tccacctgtt tgggcatgtt gcgaaagatc tcgtagactt tggcagcttc ggggctgatc
     2641 atcctcatct actccttgtc gaacagcagg gccaggggtt gagtcttaca tccggaggcg
     2701 gcacctcttc gggatcaatc atagtacaga ggctagttac cgagaacgtg cgagtacatg
     2761 ttctcccgcg gtcgatacga ggaccttagt gcggtagact ctgggttcga gggcccgtca
     2821 tgcgcggcct ttttcgttgc ttttatctct cgcccacggc gagatgagtg tgtcgggaga
     2881 accattggag tatcgcaaca tcgccatcat ctcgcacgtc gatcacggca agaccacgct
     2941 cgtggacggt ttgctcaggc aaactctgga cctcgggcac gggcagcaga tctccgatcg
     3001 tgccatggac tccaacgtgc tggagaggga gcggggcata accatactcg ccaagaacac
     3061 ggcggtggag tacgaaggcg tcaagatcaa catcgtcgac accccaggcc atacggactt
     3121 cggcggcgag gtcgagcgcg tcctcggcat ggtggacggc tgcctcctgc tagttgacgc
     3181 cgccgagggt cccatgcccc agacgcgctt cgtgttgcgc aaggccatag agctcgggct
     3241 caagcccatc gtggtcgtga acaaagtgga ccgggctgac gcccgtcccg acgaggtcgt
     3301 caacctgacc ttcgacctga tggccgacct cggcgccacc gacgagcagc tggatttccc
     3361 catcctgtac gccgttgccc gcgagggcag ggccttccgc gacctggacg agccgcggga
     3421 cgacatgagg gagctcttcg agacggtgct tgaggagatc cccgccccag aggtggacct
     3481 cacggccccc ttccagatgc tcatatccaa cctggactac tccgagtacc tcgggcggat
     3541 cgtggtcggg cgcgtaagga ggggcgaagt taggcgcggg gagtctatca acctgatcca
     3601 cacggacgga acgatgacga ggacgcgcgt ggtccagccc ttcacacacc tcgggctcga
     3661 gcgcatcgag gccgatgagg tgggcgccgg cgacatcgtg gccctctccg gcatcgagaa
     3721 cgcgcagatc ggggagaccc tggccgatga ggcccaaccg gaggcactcc ccgccatcag
     3781 cgtcgatgag ccgacggtga gcatgacctt cggcccaagc acgtccccct tcgccggcaa
     3841 agagggaagg ttcgtcacgt cgcgccacct cagggaccgc ctgaggcggg aggtccagac
     3901 caacgtatcc ctgcgcgtgg aggagctacg ccccgacgag ttcgaggtct ccgggcgtgg
     3961 cgagttgcac ctttcgatcc tcctggagac aatgcgccgc gagggttacg aggtccaggt
     4021 cggcgcgccg cgggtcatcg tgcgggagat cgagggcaag aagtacgagc ccttcgagcg
     4081 gctcgtaata gacgttccgg agcggttcgc ctccacggtc atcggggtga tctccggccg
     4141 caagggccag ctcgtcgacg tcgagcctca gggctcacgc acccgcgtgg agttcgagat
     4201 cccggcccgc gccctcttcg gcttccgcac gcagttcctc tccatgacgc agggcgaggg
     4261 cataatgagc cacgtcttcg acggctacgc gccgtggtcc ggcgacctca agacccgccg
     4321 gaacgggagc ctcgtagcct ccgaagcggg aagcgccttc gcctactcca tctggaagct
     4381 gcaggacagg ggcagcttct ttatcaagcc ttccacagag gtctacgtag gcatggtcgt
     4441 cggcgagaac aaccgcgaga acgacctcaa cgtcaacgtc tgcaagaaca agaagctaac
     4501 caacgtacgc tcggccggcg ccgacgacgc cctgaacctg accccgatcc gcaagctgac
     4561 cctagaagac gccctcgaat acatcggcga agacgagctg gtcgagatca ccccgaaaaa
     4621 catccgcctc cgcaagaaga tcctcgaacc cgggatgcgc aagtagcccc ggtccttcgc
     4681 ccgaggccaa cgtgtcgcac ataaggtcgg cgctcgcaaa ggcttgcgcc acggtgagta
     4741 ttgcgccaat atccatgcac gatgaccctg catccggcgc ttagagagct gatctacgag
     4801 aagctggcca acaccaccgc gccgcagtgg cgtctgccga tagggcaagt aaggtcctcg
     4861 ttccgtgcct tctggtctcc ggccgtgacc ggcgctcccg tggaggttgc gagcgtggag
     4921 aacaggaccg tggatacgga ggcgggcgcg gtgccggtgc gcgtgttcac gccggaagcc
     4981 gatagtcggg tgccgatcat gatgtacttc cacggcggcg ggtatgtgaa ggggggcctc
     5041 gacgagaccg acgtgttctg ccgcaggctg gccaggacca cgggctacat cgtcgtctcg
     5101 gtcgaatacc gtctgtcacc agagcatcgc ttcccggccg ccctcgacga cgcgtacgct
     5161 tgtagtctgt gggcttacca gaacgcggaa gagcttgggg ggactagcga ctctttcagc
     5221 gtctccggcg agagcgccgg cggcaacctg gctgcggtta cctgcctgat agcaagagcg
     5281 gatgagaaga tcgagatacg acgccagatc ctcctccaac ccgtcgtaga cttcacgctc
     5341 tcctttccct ccatcggcat gccagccacc gagtgcctgg ttccgcgcga cgacctggcc
     5401 tggtactacg gggagtacta ctcaggagat accagggact tcagggtctc ccccatcttc
     5461 gccgaggacc tctccagcct gccgccggcc ctgatcatca ccgccgagca cgacaccctc
     5521 cgcgacgaag gcgaagccta cgccgacagg ctccaggtcg caggcgtgcc gacacagtac
     5581 agttgctaca gcggcatgat ccacggtttc ttgctgctaa ccgggctggt ggacgaagct
     5641 cagcaagcca tcgacgagat cgccgctttc acgaaggcca aaatctgacg gttgacagcc
     5701 tggtttcagg acacgcgggt gttcgtggag gaagtcgaaa gcgcctggat cctgtgtctt
     5761 atgtgcacca ggatcgcggc gtggattgcg acggccacga ggatgcccag gacgttggcc
     5821 catacccctg ctcccgcgga gttgagcatg aagaggctgt agatcccgag cggtaggaac
     5881 aggattatcg cggtccagag gccggggttg taggcgcgca ggacgacggt gcctactacg
     5941 tgcacgaccg cgtttacgag ggtcaggtag accgagatca ggccgagcgc cgggctcacg
     6001 tacatggcaa ggtagagggc gatcacgttc accatccaga cgccaacgac gttgatcacg
     6061 agcgtcgcct ccggcgttag cgcgtccctt ccgtccgcga ccctgtcgtt gacgaaccgc
     6121 cgaaacctgt ctccggcatg ctcttcgagc tggtgcaaca tgtaaacggg tagctggaag
     6181 tagatcacac cgataaccag ccccatcccg gaaacccaca acggggcgag cgcaagcagc
     6241 agcaaccccg cgagaaaacc cgcataaggc cactcacgta gaagccagcc catcgaacgg
     6301 cggtagagcc tccccatgac ggcagtgtag tgtcggcgtc ggaacacagc aacagattgc
     6361 gcctcccgac ccgggaatac ggcccgtgaa acagttcgca ccggacgcaa acccatacgt
     6421 tttaccgtgt cggggggcgg gtatcgttca ggttgtcgtc gggacgtttc gatctccggc
     6481 gacggattcg aaaaagtagt tgcactacca tttgatggga aggtttcaga acatggatgg
     6541 aatagaaggc aaagtagtag tcgtaaccgg tagcagcagc ggtctgggtg aggccaccgc
     6601 caagctgctc gccgagcgcg gtgccaggat catgctaggc gcgcgtcggg aagagaacct
     6661 gcagaggatc gcctccgaga tcagggacaa cggcggggag gcgtcctaca gggtaacgga
     6721 cgttacggac cgagcccagg tagaaaacct ggtctccggc gccgtcgacg agttcggcag
     6781 gatcgacgtc ctggtcaaca atgccggcct gatgccgctc tccccgctcg acgcgctaaa
     6841 ggtggacgag tgggagcgta tggtcgacgt caacatcaag ggcgtcctgt acggcatcgc
     6901 cgccacgctt ccgaggatgc aggagcagca cgaagggcag atcatcaacc tctcctcggt
     6961 ggccggtcac gtcgttccgc ccaactcggc cgtctacagc ggcaccaagt tcgcggtgtg
     7021 ggccatctcg gagggcctca ggcaggaggt tggtgagaag atccggacca ctaccatctc
     7081 gcctggcgcg gtccagagtg agctcacgga ccacatcacc cacgaggaga cggcggaggg
     7141 taccagtgag ctcgatgaga tggcggtaga cgccagcgcg gtagcccgtg ccatcgccta
     7201 cgccatcgag cagcccaggg acgtggacac caacgagatc atcctgcggc ccaccgccca
     7261 ggccctttaa accaggcttc tctacggccc ggtcacacca cgtggccggg ccccgtttta
     7321 cgcctggggc aaccccatca cccggggtat gaggtagcgt tatgacggga tcaacatccg
     7381 ccgaggtgcg gcagcaggag aaccccacag ggagttccga cgtcagcacg ttgctcgtgg
     7441 gcctgatcgc gttgtgcgcc ggggtcacgg tctcgaacct gtacctgacc caaccgctgt
     7501 tgccgctcgt ggcggagagc ctgcgcgtgg gcccttctca ggtagggttt ctgccgacca
     7561 tcgggcaggc cggttacgcg ctcgggatac tcctgatcgt gccactcggg gacgtgctgc
     7621 gccgcaaggg gctgcttacg ttcgtcctcg tcgggcttat cgtggcgctc gccggcgctg
     7681 ccctggcccc ttctctaggc ttcctgatgg cggccacgct cttcgtcagt tccctgacga
     7741 tactgcccca gatcctggtc cccctcgcct cccagatagc gggcgacgag caccgcgggc
     7801 gggtcctggc gatcatcggg gcgatgatga cgtgcggcct gttcgcctca cgtagcctgt
     7861 acgggcaggt gggcgacgcc ttcggatggc gcgcctccta cgtggtggcc gccgtcctct
     7921 cggccgtcgt aggggcggtc accatcttcg tgctcccgaa agagaagggc aaggagggcg
     7981 agacctacgc gagcctcctg aggtccctgt ctacgctgct ggtcgaggaa ccgacgctcc
     8041 gcaaagcatg cctcctgcag ggcaccatct tcgccacctt caacatgttc tggaccacga
     8101 tagtcttcac gctcaccagc agcccctacg ggttctccac cggcacggcc ggcctcttcg
     8161 gtctgttcgg tatcctcagc gccacgagcg cgccgttcgt cgggaggctc atagaccgtc
     8221 gcggtcccat cccgaccctc ggagcttgcc tgctcgtcac cacctgctcg gccgccgcct
     8281 tcctgctcgg cgggacgacg atcttggcgc tcatcctcgg catagtcctg ctcggcgtgg
     8341 ggatgcaggg cgggatgagc gccaaccaga cgcgcgtcct caccatccgt cccgaggccg
     8401 gaagccgcat gaacaccgtg ttcctggtcc tgatcttcgt ctgcggtgcc gctggaggcg
     8461 cggcgggttc ggccctctac caggctggag gctggcccct ggtctgcatc tccggcatcg
     8521 gtgtcagcac cgcgggcttt atcgcctggc tcatcctcga ctacaggggc ttcaggaact
     8581 agtcggtggt ccaacccgac cgcccaacac gactccccat cggctcaagg agccacgctc
     8641 cgtgtcatca gcaccacgta cccttcgtac gggtagctcg ggtgtggcac ggtcgggagg
     8701 cgggcgacct cccggaagcc gtggcgctcg tacagcctga gggcgccctc gttctgctcg
     8761 aatacctgga ggctcaactc gctgcaaccg gcaccggagg cgagcgcggg ggcggcctcg
     8821 aggagcttgg tcccgaggcc cgtgccgcga tattcggggt acgtggcgat gatgtttatg
     8881 tagaaagttc ccggcactct ttgctccagc tcctccagcg ggcgcacgag gtcaggcacc
     8941 tcgtccaggt tcacgtcgct gtgctccggc aaagggtaag cgagcatcag ggccgccacc
     9001 ctgccgttaa cctcggcgac cacggcgttg cggtacgaga agttggcatc ttcgcgcagc
     9061 gcccgctcga tgccagcctc tacaggggat tgtccttctt tcgcactctg ggaccagagg
     9121 tagccgggga tgccgtgccc agcctggtcg ctcaactcgg cgatggcgcg ggcgtcgcgc
     9181 ctcgtcgcgc gacgcaacgg cgggcgtagc cctcccatgc tggagtcgtc cgtgcctctc
     9241 atctagtcgc tccttccctg cgaacaccga acagcgcgac gccctatcgt ccggcctgcg
     9301 gtcatgcggt gaacccccgt gtcgaggtcc gatgctccct gctattctcc tttcgtccag
     9361 gggtcaacgg gcgctaacag ccggtacttt acttcgtctg gctctcgccc tctgtatcgg
     9421 ccagctcacc gatgatgagt ttggggatca cggtggccgg cgtgttgccg tcgggtgcag
     9481 catacaggtt ggtaaggact atgatcgtca cacccgtgtc ctcggaaccc gaacacgcct
     9541 acgcaaagat acataccaga agagtagcag ctagaaatac gaggtgaggc aggcgtctac
     9601 tcatctgttc cggtttctct ggcatcaact tcccttctcg aaaaccaagg ccgtcgctgg
     9661 tcgcgacaca gcaacctcat aacgtcggcc ccaacgtcgc gatagagagc ccacgaatct
     9721 ggtaggagct gaatccggga ggttgaagaa catgcaacgt cgtgccccgc cgaagcgttc
     9781 gggtcgagcg tccgcggacc gctgtagcaa cgaggctcaa gtgaccgcgt gggaggagac
     9841 tacaccctat tcctgttggg tgaggcccgt agcccttcct gaagttctct ttccgactat
     9901 gccactctag tctctggcgc cgtcgatgtg gcgaacgtgg gcgctggcga gaatttccgg
     9961 gtccatcagg cgcgcgttga cgcccatgcg gttgaattcg cgatccaccg gaaaccagtg
    10021 cgagatgcag ccgcaggttc gacagtgatg gaactcgatc atcctgtcat tccacatgta
    10081 gatgttcgtg ggtaggtccg agggcaagag gcgtacctga tccaacgcgt agtaggccca
    10141 caagacaccg tagcgcctgc agatcgagca gttgcactcc gcaacctcag atagcggagc
    10201 atcgatctcc agtcggacgg caccgcaatg acacgaagcc tctatcatct cccccacagc
    10261 tcctttctct tccgttacga tcgcggaacc ggaaagtaac acgagacggg ttacgagacc
    10321 ggctggcgca agtatacgcg cggcccgggc gattccagcg ctgaggcaag actaccgggc
    10381 acttttcgtt ctattcggtg gtgccagcgg cgacttgccc ggtactattg ctgcacgctc
    10441 ctggcggttc catcggggag ttatggggtg gtggcttcgg acgcggcaca gggttctgcg
    10501 agagaggatg agacggggac atggaatctt tgacctggga tcaggtaaac gcctggcggc
    10561 tggcgcggca cggccttgcg ccgcgggtgg ggtttctgga ggccgcgagg cgcatgatcg
    10621 gtgtgcaggc gcaggtaatg tccgccgccg aactttcgtt gtgggcgcgt gtcgacgggc
    10681 tgcgtccatc cgacgttcaa accgcgcttt ggcaggaccg cacgctggtc aagacgtggg
    10741 cgatgcgggg tacgctgttc ctgatctccg ccgaagacct gccgctcgtg gtggccacgc
    10801 gccggatccg gaaagcgcgc tactgggtca agcatttcga gtcctacggc atccacgagc
    10861 cggagtacga ggcgcttctc gatgccatac cgcaggtgct gggcgacgag ccgatgacgc
    10921 gcgaacaact cgctgtggcg gcagcggagc acaccggcat cgacgggctg ctgccggcgc
    10981 tagccgaatc tagctggggc acggcgctaa aaccttcagc tttcaggggc gacctctgtt
    11041 acggcccgac cctgggccgc aacgtcgcct tcgtacgccc aagcgaatgg ctcgacggct
    11101 ggcgtgagat cgagccggag gtggcggcgc ggcggatcgt ccgccgctac ctgagcgtct
    11161 acggcccggc cacgccgcag gacttcggcc gctggtgggg tgccggtacg aggctggcca
    11221 cgaagacgtt cagggagttc ggagatgagc tggaagcggt ggatatcgaa ggccacagag
    11281 cgttcgcgct tcgctcgtcg ctagagccga tggagagcct ctcggcggcc ggcgtagtgc
    11341 gcctgctgcc gatgttcgac gcctacaccc atggttccat cgactacgag caacttcttc
    11401 ccacggaatt ccgacgcctg atcttccgtc cgcagggttg gatctcggcc gtcgtgctgg
    11461 tagacggacg catagtcggc gtatgggact acatgaccgg aagctcggtc acgacggtga
    11521 aggtccgcat gttcgcaaca ccagacgcca ggacccgcga cgcgatcgtg gccgaggccg
    11581 agcgcctgaa cgacttcctt gacaccaggg cgaccgtaga gttcgactct gcctgatcag
    11641 aacaagcctt gcgcgtgagc aagccggcaa gaagagacat ctagagaccc tatacctgtt
    11701 gcctgttgat aacggggggc gcttcgcgaa gcgcccctac agggggtttg tgggctgtag
    11761 tttttcgtag gctttacgct ttcccagaac gtagcctgac taccaagggc tgtggatcct
    11821 gtccgcgctt acggtgagga tgaggcgcac ctgatcacga cccccgccac gggaccactg
    11881 gtagggggca ccgaggtatc tctgggagag catttcgatg tgctcggcac caccgttggc
    11941 ggtgatgtcg accacgtgac cgcgaaccga atagtacctc agggggttat ccgggtcgga
    12001 gacggtaacg gccacgcgcg gatctcgccc gacgttgaga accttctgat aaccctggac
    12061 cgtgttgatc agaatatgct cgccgtcggt gtcgacccag gtctgggtga gctggggcga
    12121 gccgtcgggc atcgtggtag caatgtagca ggtactcggc tggcgtagca gagagatcag
    12181 agcttctggt agttccacgt tgggcctccg gtcgggtggg tatcggacga tgatgttaga
    12241 tagatgtcgt cgtggccagg atacgacgga tcacgtcccc gcaccaccag cgcgttctga
    12301 tggggctacc ctcgctctgg cgtagcggaa gaccgccccc cgaagggggc ggctctgctt
    12361 ctcgcgtgtg ggtcatacgc tctttgtatc gtctccggcg agtacccggt agagctgcct
    12421 tcgcacctcg gtcaggaggc ggcgggcctc ggagatctgg gcgctgtctc cggcctgcgc
    12481 cacctgcatg gtggccgtgg cgacctgacc cacgaggtta cgcagctcga gcaagtcctc
    12541 cccatagtcc tcggccgccg acttccacgg ggtcccgagt ttgtccctgt gctcccgcac
    12601 gtaggtatcg ccctcatctg tgaggtgggc aaccttgcgg ccatccgcct tctcgacccg
    12661 gacgagcccc tcatcctcca gttgctgaag ggcgggatag acggagccag ggctcgggtt
    12721 ccagaccccc ccgctacgct cgccgatctc ctggatgatc tggtacccgt gcatgggacg
    12781 ctccgagaga agaacgagga ccgcgccccg tacgtccccc tgccccatcc gcctgggacg
    12841 gtgcccgaac ccacccccac gaaatccccc aaaagggaac cccgccggtc catgaccccc
    12901 gggcccatga cccccaaagc cacgacgggc ggccatggcc atccgcaagc gacgccccag
    12961 gtgctcgtgc agccctccaa ccatcggtcc tcc
//
LOCUS       DBNGVD010000090         2801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2570318, whole genome shotgun sequence.
ACCESSION   DBNGVD010000090 DBNGVD010000000
VERSION     DBNGVD010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2570318"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="AC16CM_02760"
     CDS             <1..164
                     /locus_tag="AC16CM_02760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138777.1"
                     /translation="WLNLSRLNLRVYFDNAPAIALYERFGFEIEGTHKSFAYRDGEYV
                     DAHVIARLR"
     gene            complement(209..757)
                     /locus_tag="AC16CM_02765"
     CDS             complement(209..757)
                     /locus_tag="AC16CM_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138778.1"
                     /translation="MRLVDWYLGLAPGQKVVVGVAAFVLVLALTYLASIVILLAAGVG
                     PEGSSSQGEEEPVPASTTPSSSASASAFADTTLEVETARWRGNVAVVEGTWRGDLSSV
                     HCDLLEGSSAGQVTDWWDRSVAARISLSDRTFSQEFVRTGGDVKQPLDPKREYWATCW
                     AQFAGGLATGDEIQIKGTPPAG"
     gene            complement(835..1830)
                     /locus_tag="AC16CM_02770"
     CDS             complement(835..1830)
                     /locus_tag="AC16CM_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138779.1"
                     /translation="MEYGRLGNTGLMVSELCLGCMTFGKEADEETSREIVNRFLDAGG
                     NFVDTADVYSNGVSEEITGRALAGVRDDVVLATKVRFPMGAGPNDVGLSRKHIVQGCE
                     DSLRRLGTDYIDLYQVHCWDAATPLEETLATLTDLVRSGKVRYVGVSNFTAWQLMKSV
                     AVSEANGFERFVCLQPQYSLVERNIEREILPVCEEEGLGVIPWSPLGGGFLSGKYRRG
                     EQPPEDSRIAGAVESMEEHWERRSTERNWAALEVVGRISEETGRSYAQISLNWLLRQK
                     GVTAPIIGARTLEQLDDNLGASGWELSEEQVEALSWVSALEDVYPYRFIREAQRV"
     gene            1958..>2801
                     /locus_tag="AC16CM_02775"
     CDS             1958..>2801
                     /locus_tag="AC16CM_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138780.1"
                     /translation="MQSSDAGKLRDPRVPRIPPLAVLILWAAGTLVFALLPPGSEWKP
                     VIATVFSLSAATFAAALLLKSAPHAEGHERSFLRLLGAGMAFRLAGNALWSASQLFDL
                     RVAWPVAPQDIAYAISYPLLAGAMLHLVALTTHRRITLVSALDAIAVMLSVGTLIWYF
                     VLGPAATGAALGGVRDATVALSQPVCDAALLFLGLVVASSKATPRLSAYLNGGFLTLL
                     LADVAYLGLRSAGPYQSGNWPEMLWALGMILLGLTATVSPEPEASAGTTRIQPWRIVL
                     YWLGP"
BASE COUNT          435 a          982 c          867 g          517 t
ORIGIN      
        1 actggttgaa cctctcgcgg ctgaacctgc gcgtctactt cgacaacgcg cctgccatag
       61 ccctctacga gagattcggg ttcgagatag aaggaaccca caagagcttc gcctaccgcg
      121 acggcgagta cgtggacgcc cacgtcatag cccggctgcg gtagcacgtc tgccgggcgg
      181 cgcggctacc ggttgggaag aaccggcgtc acccggccgg gggcgtacct tttatctgga
      241 tctcgtcacc cgtggccaat cctccagcaa actgcgccca gcatgtcgcc cagtactctc
      301 tcttcgggtc gaggggttgc ttcacgtctc cccccgtgcg gacgaactcc tgcgagaaag
      361 tcctgtcgga aagcgagatc ctggctgcca cggagcggtc ccaccagtcg gtgacctgcc
      421 cggcgctgct cccttcgagc aggtcacagt gcaccgagga gagatccccc cgccacgtac
      481 cttcgaccac agccacgttt cctctccagc gtgccgtctc cacctccaac gtggtgtcgg
      541 cgaaagcgga ggcgctggcg ctcgaagacg gcgtggtgct cgccgggacg ggctcctcct
      601 caccctggga agaggagccc tccggcccta ccccggctgc cagcaagatc actatgctgg
      661 ccaggtaggt aagggcgagc accaggacga aggccgcgac acccacgacg accttctgtc
      721 cgggcgccag ccccaggtac cagtccacca gacgcatccg cgtatattag cccgatcccc
      781 gtccgggtac cccgacgggg tcttcgggtt gcagcggccc cggggagacc attcctatac
      841 ccgctgggcc tcgcggatga agcgatacgg gtagacatct tccagggcgc tgacccagga
      901 gagggcttcc acctgctcct ctgaaagctc ccagccgctc gcgccgaggt tgtcgtcgag
      961 ttgctcgagc gtgcgggccc cgattatggg ggcggttaca cctttttgcc tgaggagcca
     1021 gttgagcgag atctgggcgt agctcctgcc ggtctcctcg gagatcctgc cgaccacctc
     1081 gagggcggcc cagttgcgct cggtggaccg gcgctcccag tgctcttcca tggactcgac
     1141 ggcgccggcg atgcgcgagt cctccggcgg ttgctcgcca cgcctgtatt tccccgagag
     1201 gaagccgccg cccagtgggc tccagggaat cacgccaagc ccctcttcct cgcaaacggg
     1261 cagtatctcc cgctctatgt ttcgctccac gagagagtac tgcggctgca ggcaaacgaa
     1321 ccgctcgaag ccgttcgcct cgctcacggc cacgctcttc atgagctgcc aggccgtgaa
     1381 gttggagacg cccacgtagc gcaccttgcc gctcctgacg aggtcggtga gggtcgcgag
     1441 ggtctcctcg aggggcgttg ccgcgtccca gcaatgaacc tggtaaaggt cgatatagtc
     1501 cgtgccgagg cgtctgaggc tgtcctcgca tccctgcacg atgtgcttgc gcgagaggcc
     1561 cacgtcgttc gggccagcac ccatgggaaa gcggactttg gtggcgagga ccacatcgtc
     1621 gcggacgcca gcaagcgcgc ggccggtgat ctcctcggag acgccgttcg agtacacgtc
     1681 ggcggtgtcc acgaagttgc cgccggcgtc cagaaagcgg ttgacgatct ccctggacgt
     1741 ctcctcgtcg gcctcctttc cgaaggtcat gcaccccagg catagttcgg agaccataag
     1801 acccgtgttg ccaagacgcc cgtattccat gcttcgctcc tcccgaattc ctgtcgccgg
     1861 aaggccgtac tctagcacag gccgtgcctc cggcgggctc actactcggg gcatggattt
     1921 gataggatgg aagggttgga gttctggagg tagcaaggtg caaagttcgg atgcgggcaa
     1981 gcttcgtgac cctcgcgttc cgcgcattcc cccgttagca gtccttatct tatgggccgc
     2041 ggggacgctc gtctttgctc tcctgccccc tggctcggag tggaagccgg tcatcgctac
     2101 ggtcttctcc ctctccgcgg ccaccttcgc cgcagccctg ctactcaaga gtgcccccca
     2161 cgccgagggc cacgagcgct ccttcctgcg actcctgggg gccggcatgg ccttccggct
     2221 cgccgggaac gcgctctgga gcgcctcgca gctcttcgac cttcgggtcg cctggcccgt
     2281 ggccccgcaa gacatagcct acgccatctc ctaccccctg ctggcggggg ccatgctaca
     2341 cctcgtggcg ctcacgacgc acaggaggat cacgctggtc agcgccctcg acgcgattgc
     2401 ggtcatgctc tcggtgggca cgctcatctg gtatttcgtc ctcggtcccg cggcgaccgg
     2461 ggcagcgctt gggggggttc gcgacgccac agtggccctc tcccagccgg tctgcgacgc
     2521 cgccctcctc ttcctcggcc tcgtggtggc ctcatcgaag gcgacgccca ggttatcggc
     2581 ttatctgaac ggcggcttcc tgacgttgct gctcgccgac gtggcctacc tgggactccg
     2641 ctcggcgggg ccctaccaga gcggcaactg gccagagatg ctgtgggccc tcggcatgat
     2701 cctgctcggc ctcaccgcga cggtctcccc tgagcccgag gcttccgcgg gcacgacccg
     2761 gatacagccc tggcgcatag tcctgtactg gctgggcccg c
//
LOCUS       DBNGVD010000091         4483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2934468, whole genome shotgun sequence.
ACCESSION   DBNGVD010000091 DBNGVD010000000
VERSION     DBNGVD010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2934468"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC16CM_02780"
     CDS             <1..803
                     /locus_tag="AC16CM_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3138781.1"
                     /translation="PNVPEALDALVVRLLARDPEDRPASAASLSEDLRRLRDELPPLA
                     DVSGNAATRRATLRTEHVHSPPTLSGGRGSGPSAGRGRRRTLAVPLVALLVGAVLIGG
                     LVWALGRTAPDRAAPEAVARKVEVPLLVGLPPDEAQKRLEKVGLDLGSRDEAPSGTIA
                     EGAVSEQDPAAGTTAEQGRAVNVVLSTGQPREPASGGSPSATPTATPSATASPDAASP
                     EARKAAEEAAKEDQKRREEARKQAQERREEAREKAQERREERAKEGGG"
     gene            complement(835..1563)
                     /locus_tag="AC16CM_02785"
     CDS             complement(835..1563)
                     /locus_tag="AC16CM_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3138782.1"
                     /translation="MRRASMLVAVVAMLVVAFAGAASAAVISCTGGRCEGTPQSDGIT
                     GTDGRDRIFALNGFDGVFAGAGRDELNGGNLGDRLIGEDGSDTYFGGSGADFFTEIPE
                     ECCEEPPYTGNDVMDGGDGSDFMDAGEGDDVLRGRAGDERGNAGGSADPAMFGDFGDD
                     KLYGGPGDDGMIGGEGADEHYGGPDSDFIDAVDFESLDTPDLVNGGDGYDVCLVNEND
                     APVVNCERIETEPNPTPVTAVGAR"
     gene            complement(1645..2445)
                     /locus_tag="AC16CM_02790"
     CDS             complement(1645..2445)
                     /locus_tag="AC16CM_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3138783.1"
                     /translation="MRRLILLLATIGATVLLASGVAYALDVRCDAASDPDTAPGQCLG
                     TPDDDEIFGTARPDVIRALNGFDDVFARAGGDEIYGGNLADYLEGGIGWDTYFGGKGA
                     DYLTEFDSSDSGKDVMNGGPNNDVLEGGGGGDILRGQAGDESDTGDPTDSAMFGDPGN
                     DELYGGTGDDAMEGEEGTDEHYGGPDDDFIDAANNETYATDAPDLVDCGDGFDTALVL
                     PNDIVDENCEDVLEVFPGEGPVVVAASGGADDADQRRLEERFLKVRGG"
     gene            complement(2675..3061)
                     /locus_tag="AC16CM_02795"
     CDS             complement(2675..3061)
                     /locus_tag="AC16CM_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138784.1"
                     /translation="MDRIRLIEIIEAHAARLSDKGRALWEELEFLRESVPDPEDPDRT
                     AREYDLMERIFELPVPEQFGISRLAELVGGLRASEEAERGGESGDGQRARGVINAAML
                     QDREEGQSVDAQMTLDRAIARLEAQD"
     gene            complement(3181..3552)
                     /locus_tag="AC16CM_02800"
     CDS             complement(3181..3552)
                     /locus_tag="AC16CM_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3138785.1"
                     /translation="MLTHDAVQRWLDAYVEAWRTYDPEAIGALFAEEATYAYHPYDEG
                     EEVLRGRGAIVADWLEEPDEPGTWEASYRPLVVEGRRAVAEGTTSYTNGDFYWNLWTL
                     RFDEEGRCARYVEWFMVPPGS"
     gene            3790..>4483
                     /locus_tag="AC16CM_02805"
     CDS             3790..>4483
                     /locus_tag="AC16CM_02805"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3138786.1"
                     /translation="MGRRVRILHEGGPAWGRVEGEEVVLEGGGRVPEARAEYLAPVEP
                     TKILAVHLTYRSRVEEYAARTPPQPSYFVKPPSTLNGHRGVIRRPRGTEFLNYEGELA
                     VVVGRRMKGVGEEEALSYVGGYTCANDVGLHDFRHADRGSMLRVKGQDGFLPLGPEVV
                     SADAFDPTDFRIRTYLNGEVVQEGGSGDLIFPVAYQLADLCRLITLEPGDVVLTGTPA
                     NSRPMEPGDVVEV"
BASE COUNT          725 a         1575 c         1440 g          743 t
ORIGIN      
        1 accccaacgt gccagaagcg ctcgacgccc tggtcgtgag gttgctggcc agagaccccg
       61 aagaccgccc cgcgagcgcg gcctcgctct cggaggatct ccgccggcta cgagacgagc
      121 ttccgccgct tgctgatgtg tcgggaaacg ccgcaacccg tcgggcgacc ctccggaccg
      181 aacacgtaca ctcgccgcct actttgtccg ggggaagggg gtcgggcccg tccgcagggc
      241 gcggccgacg gaggaccctc gcggtgcccc tggtcgccct gctcgtgggc gcggtcctga
      301 tcggcggcct cgtgtgggcc ctggggcgga ccgcccccga ccgggctgca ccggaggccg
      361 tcgcgcggaa ggtcgaggtg ccgctcctgg tggggctgcc gccggacgag gcacagaagc
      421 gcctggagaa ggtggggctc gatctgggtt cccgggacga ggcccccagc ggcacgatcg
      481 ccgagggggc ggttagcgag caggatcccg ccgcgggtac cacggcagag cagggccgcg
      541 ccgtgaacgt ggtcctcagc acaggccagc ctcgagagcc agcctccggc ggctcgccct
      601 ctgccacacc gacggctacc ccctcggcta ccgcttcgcc ggatgcagca tcgcccgaag
      661 ccaggaaagc ggcggaggag gcggccaagg aagatcagaa gagacgagaa gaggcgcgca
      721 agcaggcaca agagcggcgc gaggaagccc gagaaaaggc ccaggagcga cgagaggaaa
      781 gggcaaagga gggcgggggg tagaacgctt agaacgctac gccctccgca aacttcacct
      841 ggcgcccact gcggtcaccg gggtggggtt gggctcggtc tcgattctct cgcagttcac
      901 gacgggggcg tcgttctcgt tcacgaggca gacgtcgtag ccgtcaccac cgttgaccag
      961 gtccggggtg tccaacgact cgaaatctac ggcatcgata aagtcactgt cggggccgcc
     1021 gtagtgttcg tcggcgccct ctccgcctat catgccgtcg tcgcccgggc caccgtagag
     1081 cttgtcgtca ccgaaatccc cgaacatcgc ggggtcagcc gagcctccgg cgttacctct
     1141 ctcgtcgccc gcccggcccc tcagaacgtc gtcgccctcg ccggcgtcca tgaagtcgga
     1201 cccatctccg ccgtccataa cgtcgttgcc cgtatagggg ggctcctcgc aacactcctc
     1261 cgggatctcc gtgaagaagt cggcgccgct gccgccgaaa taggtgtcgc taccgtcctc
     1321 accgatcagt cggtcgccga ggttgccgcc gttgagctcg tcacggccgg ccccggcaaa
     1381 caccccgtca aagccgttca aggcgaagat gcggtctcgc ccgtccgttc cggtgatccc
     1441 atccgattgc ggggttccct cgcagcgccc gccggtgcag gagatcaccg ccgccgaggc
     1501 cgcccccgcg aaggccacca cgagcatggc caccacggcc accaacatag atgctcttct
     1561 catcgctcct cctttggagg accggggctg ctattacgcc ccggcccggt gtttcgtgcc
     1621 gcctgagatc gccggcccgc gatcttagcc gccccgcact ttcaggaagc gctcttcgag
     1681 ccggcgctga tctgcgtcgt ctgctccccc ggaagccgcc acgacaacgg gaccttcgcc
     1741 ggggaagact tctagcacgt cctcacaatt ctcatccacg atgtcgttgg ggagaaccag
     1801 cgccgtgtcg aagccgtccc cgcagtccac caggtcgggc gcatccgtgg cgtacgtttc
     1861 gttgttcgcg gcatcgatga agtcgtcgtc gggtccgccg tagtgctcgt cggtgccctc
     1921 ttctccctcc atggcgtcgt cgccggtccc accgtagagc tcgtcgttgc ctgggtcgcc
     1981 aaacatcgcc gagtccgtcg gatccccggt atcgctctcg tcgccagcct ggcccctgag
     2041 gatgtcgccg cccccgcctc cctccaggac gtcgttgttg ggaccgccgt tcatcacgtc
     2101 cttgcccgaa tcggaggagt cgaactccgt gaggtagtcg gcgcccttgc cgccgaagta
     2161 ggtgtcccaa cctattccgc cctccaagta gtcggcgagg ttgccgccgt agatctcgtc
     2221 cccgcccgcc ctggcgaaca cgtcgtcgaa gccgttcagg gcgcggatca cgtccggacg
     2281 cgcggtgccg aagatctcat cgtcatccgg ggtacccagg cactgcccgg gggcggtgtc
     2341 gggatcactc gcggcgtcgc atcgcacgtc cagcgcgtag gccaccccgc tggccagtag
     2401 caccgtcgcc cctatcgttg ccagcagtag gatcagtcgt ctcaccgatg cctccttcgg
     2461 tgggtggcat gatgcctacg cggcacacct cgtcgatcaa cgaagataag tctggaggat
     2521 tgtaagccct ggtgcaactc cgtagccccc tcgcccgcgc ttctccgcgc acatctcgcg
     2581 ggtctgacca gggcactcgg ctgtccccac cacggtgagg tgcgtgcagc ggtcgtcttg
     2641 cgcgctacag gcctcctggt tcggcacaag cgctctagtc ttgcgcttcc aaccgggcga
     2701 tcgctctgtc gagcgtcatt tgcgcgtcta ccgactgccc ctcctcgcgg tcctggagca
     2761 tggcggcgtt gatcacgcca cgagccctct ggccgtctcc ggactcccct cctcgctccg
     2821 cctcctccga ggcgcggagg ccgccgacca gctcggcgag ccggctgatc ccgaactgtt
     2881 ctggcacggg caactcgaag atccgctcca tgaggtcgta ctcgcgggcc gtcctgtcgg
     2941 gatcctccgg atccggcacg gactccctga ggaactccaa ctcctcccac agcgccctgc
     3001 ccttatcgga tagccgcgcc gcatgggcct cgatgatctc gatgaggcgg atcctatcca
     3061 cgccccttcc tttctagcgg ttcgggttcg aatagcaccc gcgggaaggt ccgagcgcgg
     3121 acctcacgcg gaggggcgaa cggggcaagc agaaccgagc cgtgaaagcc cggggagcga
     3181 tcagcttccg ggcgggacca tgaaccactc cacgtaccgc gcgcaccggc cctcctcgtc
     3241 gaagcgcaac gtccacaggt tccagtagaa gtcgccgttc gtgtagctcg tggttccctc
     3301 ggccaccgcc cgccgtccct cgaccaccag gggacggtaa ctcgcctccc aggtgcccgg
     3361 ctcgtccggc tcttccagcc agtcggcgac gatcgcccca cggcccctga gcacctcctc
     3421 gccttcgtcg tagggatggt aggcgtaggt ggcctcctcg gcaaagaggg cccctatggc
     3481 ctcgggatcg tacgtgcgcc acgcctccac gtaggcgtcg agccaccgct gcacggcgtc
     3541 atgagtcaac atggtcctcc cccggcatcg gagttcgggc gcttgatccc cccaacaccc
     3601 ctgcagtctt ttcgccgata gacacgctca tcacccgccc gacatcctac gccaccagga
     3661 ccgtcgctgc tcccacacca cgaacacccc gtagcaatcc cgagccggaa aacccagagc
     3721 caagctcgtc ccagcatctt gacagggacg gagtttttga ataatgtatc caataggaaa
     3781 ggagaattca tgggacgaag ggtaaggatc ctgcacgaag gcgggcctgc ttggggcagg
     3841 gtcgaggggg aagaggttgt gctggagggt gggggacggg tccccgaggc tagggccgag
     3901 tatctggcgc cggtggagcc gaccaagatc ctcgccgtcc acctgaccta caggagccgt
     3961 gttgaggagt acgcggcgcg cacgccgccg cagccgtcgt acttcgtaaa gccgccgagc
     4021 acgttgaacg gccatcgcgg cgtgatacgg cgtccccgcg ggaccgagtt cctcaactac
     4081 gagggcgagc tcgctgtggt cgtaggacgt cggatgaagg gggttgggga ggaggaagcc
     4141 ctctcctacg tcggcgggta cacgtgcgcc aacgacgtgg ggttgcacga cttcaggcat
     4201 gccgatcgcg ggtccatgct cagggtaaag gggcaggacg ggttcctgcc gctcgggccc
     4261 gaggtggtct cggcggacgc cttcgatccc acggactttc ggatccggac gtacctgaac
     4321 ggggaggtgg tgcaggaggg gggatctggg gacctgatct tccccgtcgc ctaccagctc
     4381 gccgacctgt gccgcctcat cacgctggag ccgggcgacg tggtactgac cggcacgccc
     4441 gccaactccc ggccgatgga gccgggggac gtggtagagg ttg
//
LOCUS       DBNGVD010000092         2174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2388109, whole genome shotgun sequence.
ACCESSION   DBNGVD010000092 DBNGVD010000000
VERSION     DBNGVD010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2388109"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="AC16CM_02810"
     CDS             <1..804
                     /locus_tag="AC16CM_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3138787.1"
                     /translation="EDLVILPGTTLENEPPNRIAEWTYRLGFGRFARNYTVHVINRRR
                     GMPPGYTTRDMAADYATVLEQELGPSHVMGFSTGGEIAQYVALDHPSSVRRLILVVSA
                     CRLSAEGREICERWQVLAREGRWQDLRTDMASVTVSGETNKRLARLFMGVFGRLIIKA
                     PSDAQDFLTTLGADLGHDTTERLGEISAPSLVIGGTEDPFYSEHTIRETTEKIPDATL
                     SLYEGVGHGVPKERKRRYEQDALAFLADHREEWSGKGERSIPTRAEETR"
     gene            801..1532
                     /locus_tag="AC16CM_02815"
     CDS             801..1532
                     /locus_tag="AC16CM_02815"
                     /inference="COORDINATES: protein motif:HMM:NF028425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR3138788.1"
                     /translation="MKISAFLFTLLVAAAFLAGACSKQGGGTQQEVGKIRNESESVDP
                     KNAQSVRAQLKMGAGELNLTGGADQLLEADFSYNVAEWKPEVTYDVSGDEGELVVEQG
                     GSGGSNLTGEARNEWEVRLNDELPTDLVVQLGAGETDLDLDSLALAGLKLQMGAGKTT
                     VDLTGDYAQSFNASIQGGVGEATVELPSGVGVKAKAQGGLGRINAEGLERVGDSYVND
                     AFGESDVTLNVEVQGGVGEINLKVV"
     gene            1573..1788
                     /locus_tag="AC16CM_02820"
     CDS             1573..1788
                     /locus_tag="AC16CM_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138789.1"
                     /translation="MPARRYKGKSREIGAAGLQLAANAQKALAKLGLAEAEAGVPRRA
                     SASEIRSWRGKVRKHCSRARWGRGRCM"
BASE COUNT          432 a          649 c          742 g          351 t
ORIGIN      
        1 gaggacctcg tgatcctgcc aggcacgacc ctcgagaacg agcccccgaa ccggatcgct
       61 gagtggacct atcgcttggg cttcggccgc ttcgccagaa actacacggt ccacgtcatc
      121 aaccgcaggc gaggcatgcc gcccggctac accaccaggg acatggcggc ggattacgcc
      181 acggtgctgg aacaggagct cggaccctcg cacgtcatgg gtttctccac cgggggagag
      241 atcgctcagt acgtggcact cgaccaccca tcatcggtga gacgtttgat cctggtagtc
      301 agcgcctgcc gcctgtcggc ggaggggaga gagatctgcg agcgctggca ggtgctggct
      361 cgtgagggac gatggcagga tttgcggaca gatatggcct ccgtcacggt gagcggcgag
      421 acgaacaaac gcctcgcgag gctgttcatg ggggtgttcg ggaggctgat catcaaggca
      481 ccatccgacg cgcaggactt tctcaccacg ttgggggccg acctgggcca cgataccacc
      541 gaaaggctgg gcgagatctc cgcgccgtct ctcgttatag gtggcaccga agatcccttc
      601 tactcggagc acaccatccg cgagaccacc gaaaagatac ccgatgctac cctgagcctc
      661 tacgagggcg tcggtcacgg agtgccgaag gagcgcaagc gccgctacga acaggacgcg
      721 cttgcgttcc tggccgacca tcgtgaagaa tggtccggta agggagagcg gtcgatcccc
      781 acacgagccg aggagacgag atgaagatat cggcgtttct gttcacgttg ttggttgccg
      841 cggcgtttct ggccggggcc tgctcgaagc agggcggtgg gacgcagcag gaagtcggaa
      901 agattcggaa tgagtctgaa tcagtcgatc ccaagaacgc ccagtccgtc cgcgcccagc
      961 tcaagatggg ggctggagag ctgaacctca ccggcggcgc ggaccagctc ctggaggcag
     1021 acttctccta caacgtcgcc gaatggaaac cagaggtgac ttacgacgtg agcggcgatg
     1081 agggtgagct cgtcgtagag cagggaggct ccgggggctc caacctcacc ggcgaggccc
     1141 gcaacgagtg ggaggttcgc ctgaacgacg aactgcccac cgatctcgtg gtccagctgg
     1201 gcgcgggaga gaccgacctg gacctggaca gtctcgcgct ggcgggactc aagctccaga
     1261 tgggggcggg caagaccacg gtggacctta cgggcgacta cgcgcaaagc ttcaacgcaa
     1321 gcatacaggg tggggtcggg gaggcaacgg tagagctgcc cagcggggtc ggcgtcaagg
     1381 cgaaagccca gggaggactg ggcaggatca acgccgaggg actcgagagg gtaggcgact
     1441 cttacgtcaa cgacgccttc ggcgaatcgg acgttacgct caacgtagaa gtgcaaggtg
     1501 gcgtaggcga gatcaacctg aaggtcgtct gaggccgtct ggcaggctgc caggatactc
     1561 tgctcggtgt ccttgcccgc acgacggtac aaagggaagt cgagggagat cggagccgcc
     1621 gggttgcagc tcgccgccaa cgcccagaag gcgctcgcca agctcggcct cgccgaggcg
     1681 gaggcgggcg tgcccaggcg cgcctccgcc tccgaaatac gctcgtggcg cgggaaggtg
     1741 cgcaagcact gctcgcgcgc gaggtggggg aggggacgct gcatgtgagc gccgaggcga
     1801 cggcttcggg caggacaacg gcggcgtgat catccacttg ccgatcggct tgccaacggt
     1861 agccggggtt ccccagaccg gatcagaacc ctcgcgacca tgctccaaac atactcgtct
     1921 aggggcgttt cggaccgctt ccatcgaagt gccgatcgag atcaagtagt tgacgggagg
     1981 ctcgacgcct gtacattaag catgcgaggc agaacagggc gcctccctgc ttcgagaatc
     2041 ccgcgagcct tgtcgggtcg gtgcttctaa gcgtcgcata acgattaggg taattgcggg
     2101 cccgttcccg tcaccacccg tccgattttg gtcatgtgtc gctgccttcg cgttccgtct
     2161 gcgccagcct gcgg
//
LOCUS       DBNGVD010000093         4435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4574265, whole genome shotgun sequence.
ACCESSION   DBNGVD010000093 DBNGVD010000000
VERSION     DBNGVD010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4574265"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1696
                     /locus_tag="AC16CM_02825"
     CDS             332..1696
                     /locus_tag="AC16CM_02825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005528455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR3138790.1"
                     /translation="MDRIVAFCKRRGFVYQSSEIYGGLRSSYDYGPLGVEMKRNIKEE
                     WWRRMVHMREDMVGLDSAIIMHPRVWEASGHTATFNDMLVESRTSKRRYRADHLIEDA
                     TGIDAEGMSPEELTRIIEEDDRIKDPLDGGRDFAPVRPFNLMFETYMGPVKTPENLAY
                     LRPETAQGIFVNFKNVLQTSRVKVPFGIAQQGKSFRNEITPGNFIFRTREFEQMEMEF
                     FVEPGTDEEWHEYWIEERYNWYLNLGLKEENLRRYEHPQEKLSHYAKRTVDIEYDYAS
                     VGWSELEGIANRTDYDLKQHAAHSGENLEYFDQATNNRYTPYVIEPAVGPDRIMLAFL
                     VDAYTEEEVNGEARTVLKLHPRIAPTKAAVFPLSKKEPVSTIARELYDDLKGDYRLSY
                     DDSGSIGRRYRRQDEAGTPFCVTVDFDTIDDKKVTIRDRDTLEQERVPIEAVRDRLKA
                     LISG"
     gene            complement(1764..3368)
                     /locus_tag="AC16CM_02830"
     CDS             complement(1764..3368)
                     /locus_tag="AC16CM_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3138791.1"
                     /translation="MMANNAAGSDDLFQRKATGLLRGWSVWDGFIYAAFSINLITLGL
                     LAFSYAPFVPDGSLILAIILAGLYLIFQTITYASLIAVMPRAGGDYVWTSRVLGGGVG
                     FVLAVTGWWFILWHWVPIYADLLNKEVFVPLAALFGLDGTVAFFSGASGLFTASMIVA
                     IVASLYVSLGMRAYARIQKFCFYGGLVGLAIMILILLFSSRADFVSAYNSGAQELFGA
                     SGNAYQETIRVGEEAGGLSTGNAVYPTLLLIPFLAFFNLYSNWGATLYGEVRGASDFR
                     KNIYAMAGALIFTSIFAVILLLLFAKTFGWDFYYAANVNFWNGEGPVAAWPYPGLLTA
                     MLLGNPVLQVLLVAVLSLWFFGWTGSVFLSSTRVVFATAFDRVLPEWVSRTTNNGVPY
                     AALLLMLIPSIPVSYAYAFGENFATYVLDAVLVIAITFLGSTVSAALMPWLKPDIYNA
                     SPIARYRVLGLPLITVVAAAFGGFLLFCLYQWFFNDLYGVNNGQSMIYMLILYAIAFA
                     MYVGFRLLRRAQGMDLAMVYDEIPEE"
     gene            complement(3378..4352)
                     /locus_tag="AC16CM_02835"
     CDS             complement(3378..4352)
                     /locus_tag="AC16CM_02835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3138792.1"
                     /translation="MISRLFSREPDTRVYFATDIHGSETCWRKFLNSGKHYEANVMVL
                     GGDMTGKALVPIVREGKDRWHATLLENRREFDGEDEVKEFESSVMRRGYYPFRTDPDQ
                     MSELSESEELRDELFHKEMLGTVERWMRMADEKLDGTGIDCFVSPGNDDQFEVDEIIS
                     SARRVRLAEGEVVEFGDFQMVSTGWSNRTPWDTYREEDEDDLAERIKKMTSQVTAPPE
                     KTIYNFHCPPYGSGLDDAPEIDENMRPKHGGRSLVPVGSTAVREAIEEGQPALSLHGH
                     IHEAKGNTRIGRTLCINPGSSYEQGQLLGAVVNLDGSNKVKRFVLTSG"
BASE COUNT          929 a         1371 c         1381 g          754 t
ORIGIN      
        1 agggtccaat cggcgggcag gcccaggagg tcgggcggca cgataaagcc aagctcggcg
       61 aacagcacgt aaagcgcgag cggcacgaaa gccctcaccc ccgcaacgga agcgagcccc
      121 acgccgagac cgaaccagac catcgtctcc atcagcacag tatatcgaat cgagaaccag
      181 gaatgaggaa ccgggcgttt ggaaccgggc ggtctggcct ggccattccg acctccgact
      241 tcccaatttg cgggtgacca gtttatactc gcttgacttt ttgatgtgat gaccttcaag
      301 gttcggaggc cgcgtggcgc agaccgtgac gatggacagg atagtggcct tctgcaagcg
      361 caggggcttc gtgtatcaga gttcggagat ctacggcggc ctgcgctcct cctatgatta
      421 cggtccgcta ggcgtggaga tgaagcggaa catcaaggaa gagtggtggc gccgcatggt
      481 ccacatgcgc gaggacatgg tcggcctcga ttccgcaatc atcatgcacc ccagggtatg
      541 ggaggcctcg gggcacacgg cgaccttcaa cgacatgctg gtcgagagcc gcaccagcaa
      601 gcgccgctac agggccgacc atctcatcga ggacgcgacg ggcatagacg ccgagggcat
      661 gagccccgag gagttgacca ggatcatcga ggaggacgac aggatcaagg accccctcga
      721 cgggggacgt gacttcgcac ccgtaaggcc cttcaacctg atgttcgaga cctacatggg
      781 accggtcaag acgcccgaga acctcgccta tctgcgtccc gagacggcgc aggggatctt
      841 cgtgaacttc aagaacgtcc tgcagaccag ccgggtaaag gtgcccttcg ggatagcgca
      901 gcagggcaag tctttccgga acgagatcac ccccggaaac ttcatcttcc gcacccgcga
      961 gttcgagcag atggagatgg agttcttcgt ggagcctggc accgatgagg agtggcacga
     1021 gtactggatc gaagaacgtt acaactggta cctgaacctc ggcctgaagg aggagaacct
     1081 ccgtcgctac gagcacccgc aggagaagct ctcccactac gcgaagcgca ccgtcgatat
     1141 cgagtacgac tacgcctcgg tcggctggtc ggagctggaa ggcatcgcca accgcacgga
     1201 ctacgacctc aaacagcacg ccgcccactc cggtgagaac ctggagtatt tcgaccaggc
     1261 caccaacaac cgctacaccc cttacgtcat agagccggcg gtcggtcccg acaggatcat
     1321 gctcgccttc ctcgtcgacg cctataccga agaagaggta aacggcgaag cgcgcaccgt
     1381 gctcaagcta cacccgcgta tagcccccac caaggcagcc gtcttcccgc tctccaagaa
     1441 ggaaccggtc tccaccatag cccgcgagct ctacgacgac ctcaaaggcg actaccgcct
     1501 ctcctacgac gattccggct ctatcgggcg ccgctaccgc cgccaggacg aggctggcac
     1561 ccccttctgc gtcaccgtcg acttcgacac catcgacgac aagaaggtga ccatccgcga
     1621 ccgcgacacc ctcgaacaag agcgcgtgcc gatcgaagcg gtaagggaca ggctcaaagc
     1681 cctcatctcg ggttaagtaa gccttcagct atcagcagtc agctttcagc ttcttttgct
     1741 gactgctgat agctgaccgc tctttactcc tccggtatct cgtcgtagac catcgccagg
     1801 tccatgccct gtgcgcggcg gagcaggcgg aacccgacgt acatggcgaa cgcgatggcg
     1861 tagaggatca gcatgtagat catggattgt ccgttgttga caccgtagag gtcgttgaag
     1921 aaccactggt agagacagaa gagcaggaac ccgccgaagg cggccgcgac gaccgtaatc
     1981 agggggaggc ccagcacacg atacctggct atcggggagg cattgtagat atccggtttg
     2041 agccacggca tcagggccgc cgagacggtc gagccgagga aggttatggc gatgacgagc
     2101 accgcgtcga gcacgtaggt ggcgaagttc tccccgaagg cgtaggcgta gctgaccggg
     2161 atagacggta tgagcatgag cagcagcgcc gcgtagggga cgccgttgtt cgtggtgcgt
     2221 gagacccatt ccgggagcac cctgtcgaag gccgtcgcga agacgacgcg ggtggaggag
     2281 agaaacacag acccggtcca tccgaagaac cagagggaca gcacggcgac cagcaggacc
     2341 tgcaacacgg ggttaccgag gagcatcgcg gtgagcaacc ctggatacgg ccacgcggcg
     2401 accggacctt ccccgttcca gaagttgacg ttggcagcgt agtagaagtc ccacccgaac
     2461 gtcttggcga acaggaggag cagtatgacg gcgaagatag aggtaaagat cagggccccg
     2521 gccatggcgt agatgttctt tcggaagtcc gaggcgcccc tgacctcacc gtagagcgtc
     2581 gctccccagt tggaatacag gttgaagaag gcgaggaacg ggataaggag caacgtcggg
     2641 tagacggcgt tgcctgtgga gaggccaccg gcctcctcgc cgaccctgat ggtctcctgg
     2701 taagcgttgc cgctggcgcc gaagagttcc tgcgcccccg agttgtaggc ggagacgaag
     2761 tcggcccttg agctgaagag caggatcagg atcatgatgg cgagccccac gagcccgccg
     2821 tagaaacaga acttctggat gcgggcgtag gccctcatac ccagggagac gtagagcgag
     2881 gccacgatgg cgacgatcat tgacgccgtg aagagccccg aggctccgga gaagaaagcg
     2941 accgtgccgt ccaggccgaa aagcgcggcg aggggcacga acacctcctt gttcaggagg
     3001 tcggcgtaga tgggcaccca gtgccagagg atgaaccacc agcccgtcac ggcgagtacg
     3061 aaaccgacac ccccgccgag cacgcggctc gtccacacgt agtcgccgcc ggccctgggc
     3121 atcaccgcta tgagggaggc gtaggtgatg gtctgaaaga tgaggtagag gccggcgagg
     3181 ataatcgcca gtatcaggga accgtcgggg acgaacgggg cgtaagagaa cgccaggagc
     3241 ccgagcgtga tcaggttgat cgagaacgcg gcgtagatga agccgtccca caccgaccag
     3301 ccccgcaaga gcccggtcgc tttgcgctgg aagaggtcgt cggaccccgc cgcattgttc
     3361 gccatcattc tccctttcta tccactggtg agtacgaacc gcttgacctt gttgctgccg
     3421 tcgaggttga cgacggcgcc gagcaactgt ccttgctcgt aggaactgcc agggttgatg
     3481 cacagcgtgc gcccgatgcg ggtgttgccc ttggcctcgt ggatgtgtcc gtgcagggag
     3541 agggctggtt gcccctcttc gatggcctcc ctgacggccg tggagccgac gggcacgagc
     3601 gagcgtccgc cgtgctttgg ccgcatgttc tcgtctatct ccggggcgtc atcgaggccg
     3661 gagccgtagg gcgggcagtg aaagttgtag atcgtcttct ccggcggggc cgtcacctgg
     3721 gaggtcatct tctttatccg ctcggcgagg tcgtcctcgt cctcctcgcg gtaggtgtcc
     3781 cagggcgtgc ggttcgacca cccggtcgag accatctgga agtcaccgaa ctcgaccacc
     3841 tcgccctcgg ccagccgcac gcgcctggcg ctggagatga tctcatctac ctcgaactgg
     3901 tcgtcgttgc cggggctgac gaagcagtct attcccgtcc cgtcgagctt ctcgtcggcc
     3961 atccgcatcc agcgctccac ggtaccgagc atctccttgt ggaagagctc gtccctgagc
     4021 tcctcggact ccgagagctc ggacatctgg tcgggatcgg tacggaaggg gtagtagccg
     4081 cgccgcatga cggagctctc gaactccttc acctcgtctt cgccgtcgaa ctcccgccgg
     4141 ttctcgagca gcgttgcgtg ccagcggtcc ttgccttccc ggacgattgg aacgagcgcc
     4201 ttgccggtca tgtcgccgcc caggaccata acgttcgcct cgtagtgctt gcccgagttc
     4261 agaaacttgc gccagcaagt ctccgaacca tggatgtctg tggcgaagta caccctcgta
     4321 tcgggctcgc gcgaaaataa tctggagatc atggtccccc ctccacctcc tcgggcaggt
     4381 cgtaccaacg tttgcggtca cctatcttgg ccctcatctt ccaaccgaac ccctt
//
LOCUS       DBNGVD010000094         5071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1295605, whole genome shotgun sequence.
ACCESSION   DBNGVD010000094 DBNGVD010000000
VERSION     DBNGVD010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1295605"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16CM_02840"
     CDS             complement(<1..619)
                     /locus_tag="AC16CM_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3138793.1"
                     /translation="MPLVDLDRFDFLYATRVKGMKSAVTRDLMATLSRPGIISLAGGF
                     PDTKAFGEEAFREISGNIAQDAAQALQYGPTAGLEAIKDVIVEVMGAEGTPARQEDVF
                     VTTGAQQGLDLIAKVFLDEGDAVLCEGPTYAGALNAFAAYRPRIAHVPMDRAGIIPIA
                     ARETLKRARTQGLHVKFLYTVPNFQNPAGVTMIAERRRELLEMARE"
     gene            complement(1106..1645)
                     /locus_tag="AC16CM_02845"
     CDS             complement(1106..1645)
                     /locus_tag="AC16CM_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3138794.1"
                     /translation="MCCTLILVRRQAEEGAYETSWCILGYGGVATGIGCRRSVGGELR
                     RNADPNVLVGTNGADFIDGKGGNDQLFGLDGRDTILGRDGDDLIEGGRGPDRLTGGPG
                     NDTIYGGGGNDAVNGGPGDDTIIVGDDVNGPKGADQYACGDGEDIVYVRSSDNSTLQD
                     EENCETTTNKEPPPAPPVP"
     gene            2007..2558
                     /locus_tag="AC16CM_02850"
     CDS             2007..2558
                     /locus_tag="AC16CM_02850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR3138795.1"
                     /translation="MKAIFVTTNEHKRREVEQILGVTLEGVDLDLPEIQAIDPVEVAA
                     EKARAARGALGDTDLPVLVEDSGLMVDAWGGFPGALTKWVMQSVGNEGLLRMLAPEED
                     RSASAVCVVALAGVDGEVRTFRCEVRGAVAESPRGDGGFGYDPVFVPGWSDETYAEMG
                     EAKNTGSHRALAFRALKEWLEKA"
     gene            2649..3713
                     /gene="pdhA"
                     /locus_tag="AC16CM_02855"
     CDS             2649..3713
                     /gene="pdhA"
                     /locus_tag="AC16CM_02855"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR3138796.1"
                     /translation="MSAENENSGNGGEETREDGGAIATSEERLVDLYREMALIRAFEE
                     ACQRGFRQGKIGGYLHVYIGQEAVATGFLQAYKDGDRIITAYRDHAHALLLGSDPKEV
                     MAELYGKGTGLVKGKGGSMHLFDVDNGLMGGYGIVGGHIPLGVGVAYAMRYEETDHIC
                     QLYLGDGAISQGAFHEAANLAGLWGKDGQCPCLFIVENNQYGMGTSVERTTAMTDLAA
                     KFDAYGIEHEKVDGMDVEAVVEVAERVVEQVRETGKPYAVEALTYRTAPHGAADFFEK
                     YRTKEEVQEWRGRDPIGMVEHKLIENDVLDEDGIEEIKDEAKELIQEAVKFADESEEP
                     PEEELYTDVYAGEDEYMGEA"
     gene            3717..4706
                     /locus_tag="AC16CM_02860"
     CDS             3717..4706
                     /locus_tag="AC16CM_02860"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3138797.1"
                     /translation="MAETKTYREALREAMVHELDNDESVVLMGEDIGVYGGTHLITDG
                     LYDEYGPRRIMDVPISENGFVGAAVGMAMMGMRPVVEMMTWNFSFLAADQIIQNAAKV
                     RYFSGGQVEVPLVIRGPNGGGVQLSAQHTHSLESFYGHFPGLKVVAPVTPNDVKGMML
                     TAIRDPNPVIFLEAGALYGTKGEVEEGDNAVPFGKARIAREGSDVTLIAYGRQVNLCL
                     RVAETLSEDGFEAEVIDLRSLRPFDEDTIVESVEKTHRAVAVQEQWRWFGVASEVAAI
                     IQDRAFDSLDAPVERVSGAEVPGPYARNLELMAFPSEKQVENAARRVMYEEEK"
     gene            4708..>5071
                     /locus_tag="AC16CM_02865"
     CDS             4708..>5071
                     /locus_tag="AC16CM_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3138798.1"
                     /translation="MPEVIMPKMGDAMEEGTLLKWLKSEGDDVAEGDPIAEIETDKVT
                     MELEAEDAGTLAQLIADEGQDIPVGEAIAFIQGEGEDVPERSEGGGEAEEEAEQGGED
                     GGGAQATATETEAPEEEEG"
BASE COUNT          992 a         1494 c         1701 g          884 t
ORIGIN      
        1 actcgcgcgc catctccaga agctccctac gcctttcggc gatcatggtc acgcctgcgg
       61 ggttctggaa gttgggcacg gtgtagagga acttgacgtg cagtccctgc gtacgggccc
      121 tcttcaaggt ctcgcgggct gcaatgggga tgatgccggc ccggtccatc ggaacatgcg
      181 ctatgcgtgg cctgtaggct gcgaacgcgt ttagcgcccc cgcgtaggtc gggccctcac
      241 acagcaccgc gtctccctcg tccagaaaca ccttcgctat gaggtccaat ccctgctggg
      301 ccccggtggt gacgaagaca tcctcctgcc gcgctggcgt tccttccgcg cccatgacct
      361 caactatcac gtccttgata gcctctaggc cggccgtggg tccgtactgg agggcctggg
      421 ccgcgtcctg ggcgatgttg cctgagatct cccggaacgc ctcctcgccg aaggcctttg
      481 tgtcggggaa tcctcctgcc agcgagatga tgccagggcg cgagagcgtg gccatcaggt
      541 ctcgggtcac cgcggacttc atgcccttga ccctggttgc gtagagaaaa tcgaagcggt
      601 cgaggtctac gagcggcaaa agtcgtctcc ctgtagcaat ggtctgtact gcggaaagat
      661 tatgtgcggc gagcccgcgt cggccggaag aggcatccgt gtaccctgcg tctccagctt
      721 gtcgaggctc tccaacctca gctcatgcat aggggcgact gtatcataaa gaatcgtccc
      781 ttcctataag ggagggccag gggagggagg ttgggatcgg gccccgcgac acctgatcgc
      841 ggagagagga gcaagtatgg cggaagataa caagtccctg gtgttgcgct acgtcgatga
      901 ggtctcgaac ggtcacgacc tagagatcat ctaagacatc gtctcgccgc gctacgcgaa
      961 ccacgcggcc aggaccgaag agtacccgca cctcgatcta gccgtagcca gctggctacc
     1021 cgggggcttg gcgtacacgc gaaaagcgaa ggccgggtcc tgaaacaggg cccggcctcc
     1081 cttgtttgct agcggagtgg cgtccctagg ggacaggtgg ggccggtggg ggctccttgt
     1141 tcgttgtcgt ctcgcagttc tcctcgtcct gcagagtgct gttgtcggag gagcgtacgt
     1201 agactatgtc ttctccgtct ccgcaggcgt actggtcggc ccccttggga ccattgacat
     1261 cgtcccctac gatgatggtg tcgtcacctg gtccgccgtt tacggcatcg ttgccgccac
     1321 cgccgtagat ggtgtcattg ccggggccac cggtcagcct gtcggggccg cgaccgcctt
     1381 cgatcagatc gtcgccgtcc cggccgagga tcgtgtcgcg accgtcgaga ccaaagagct
     1441 ggtcgttgcc gccttttcca tcgatgaagt cagcaccgtt ggtgcctaca aggacattgg
     1501 gatcggcatt cctacgaagt tccccgccaa cgctacgccg gcacccaatg ccagtagcaa
     1561 cgcccccgta accaagaata caccaactcg tttcatatgc tccctcttct gcttgcctac
     1621 gaaccaggat caacgtgcag cacatatcgt tcttccttgt aacgctcctt tccgcgtggt
     1681 cctcgttgcc ggtacggtag cgggatgcta tgtactagca ataaacaaga cccagcgatg
     1741 agtactatat aagctgccac accaccgaaa catcccccaa acgtcgtata tttgatgctg
     1801 cttgcggttc ctaacgtgta gcgagcttcc ctgagtctcc acggcaccgg ccggcgttgg
     1861 cccgcgcgcc gacaatgagg ttgaccagtt tccggcctgg tcctctctgc aatttgtcgt
     1921 aggactttca ccacgttatc cgagtcccgt ggttacactc tagatcgcgg ttagagtctg
     1981 tgggacgcga ctagcaggag acgtctgtga aggccatctt cgtaaccacc aatgagcaca
     2041 agcgtcgcga ggtagagcag atactcggcg tcacgctgga aggcgtggac cttgatctgc
     2101 ctgagataca ggccatcgac ccggtcgagg tggcggcgga gaaggcccgc gccgcccgcg
     2161 gggcgctggg ggatacggat ctccctgtgc tggtggaaga ctcggggcta atggtcgatg
     2221 cttggggcgg ctttcccggg gcccttacca aatgggtaat gcagagcgtc gggaatgagg
     2281 gactgctcag gatgctcgcc ccggaggagg accgttccgc gagcgccgtc tgcgtggtag
     2341 cgctcgcagg ggtagatggg gaggtgcgca ctttcaggtg tgaggtgcgt ggggccgtcg
     2401 ccgagagtcc gcggggcgat ggcgggttcg gctacgatcc cgtgttcgtt cccggatggt
     2461 cggatgagac ctacgcggag atgggtgagg caaagaacac gggctcgcac cgggcgcttg
     2521 ccttcagggc cctcaaggag tggttagaaa aggcgtagga gcggggtaca tgtaaagggg
     2581 gatggatgcc agagtcgcgc ttttggtatt ctgtcgctta ttgtacctct gagagggaag
     2641 gggccgtaat gtcggcagag aacgagaact cgggaaacgg gggcgaagag acccgcgagg
     2701 acggtggtgc catagccaca agcgaggagc gcctcgtaga tctatatcgc gagatggccc
     2761 tcattcgggc cttcgaggag gcgtgccagc gcgggttcag gcagggcaag atcgggggct
     2821 acctccacgt ctacataggg caggaggccg tcgccacggg cttcttgcag gcctacaagg
     2881 atggcgacag gattatcacc gcctaccggg atcacgccca cgcgctcctg ctaggtagcg
     2941 atcccaagga ggtcatggcc gagctctacg gaaagggcac gggcctcgtg aagggcaagg
     3001 gcggctccat gcacctcttc gacgtggaca acgggctcat gggcggctac ggcatcgtcg
     3061 gcgggcacat accccttggg gttggcgtcg cctacgccat gcgctacgag gagaccgacc
     3121 acatctgcca gctctacctg ggcgacggcg ccataagcca gggcgccttc cacgaggcgg
     3181 ccaacctggc cgggttgtgg ggcaaggatg ggcagtgtcc ctgcctgttc atcgtcgaaa
     3241 acaaccagta cgggatgggc acctcggtcg agcgcaccac cgccatgacc gacctcgccg
     3301 ccaagttcga cgcctacggc atcgagcacg agaaggtgga cggcatggac gtggaggccg
     3361 tcgtagaggt tgccgagagg gtcgtcgagc aggtcaggga gaccggcaag ccgtacgcgg
     3421 tcgaggccct cacctacagg accgccccgc acggcgccgc cgacttcttc gagaagtatc
     3481 gcaccaaaga agaggtccag gagtggcggg ggcgtgatcc cataggaatg gtcgagcaca
     3541 agctcataga gaacgatgtg ctcgatgagg acgggataga ggagataaaa gacgaggcca
     3601 aggagcttat ccaggaggcc gtgaagttcg ccgacgagag cgaggagccg cccgaggagg
     3661 agctctacac cgacgtctac gccggcgaag acgagtacat gggggaggcg taagccgtgg
     3721 cggagaccaa gacctaccgc gaggccttga gggaggccat ggtccacgag ctcgacaacg
     3781 acgagagcgt ggtgttgatg ggtgaggaca tcggtgtcta cgggggcacc cacctcatca
     3841 ccgatggcct ctacgacgag tacggcccca ggcgcattat ggacgtgccc atctccgaga
     3901 acgggttcgt cggcgccgcc gttggcatgg ccatgatggg catgcgaccc gtcgtcgaga
     3961 tgatgacctg gaacttctcg ttcctggcgg ccgaccagat catccagaac gccgccaaag
     4021 tccgctactt ctccggcggc caggtggagg tgccgctcgt gatccgcggc ccaaacggcg
     4081 gcggcgtcca gctctcagcc cagcacacgc actccctgga gagcttctac ggccacttcc
     4141 ccggcctgaa ggtcgttgcg cccgtaaccc ccaacgacgt caagggcatg atgctgacgg
     4201 ccatacggga tccaaacccg gtgatcttcc tcgaagccgg cgccctgtac ggcacgaagg
     4261 gcgaggtgga ggagggcgac aacgccgtgc cgttcggcaa ggcccgcata gcccgcgagg
     4321 gctccgacgt gacgctcatc gcctacggcc gccaggtcaa cttgtgcctg agggtggcgg
     4381 agacgctctc cgaagacggc ttcgaggccg aggtgataga cctaagatcc ctgcggccct
     4441 tcgacgagga caccatcgtg gagtcggtgg agaagacgca cagggccgtg gccgtgcagg
     4501 agcagtggcg gtggttcggg gtggcgagcg aggtggcggc gatcatccag gacagggcct
     4561 tcgattcgct ggatgccccc gtggagcggg taagcggcgc cgaggtccca gggccgtacg
     4621 cacgcaacct cgagttgatg gccttcccga gcgagaagca ggtcgagaac gcggcccgta
     4681 gggttatgta cgaggaggag aagtaaagtg cctgaagtga tcatgcccaa gatgggcgac
     4741 gctatggagg agggcacgct cctcaagtgg ctcaagagcg agggcgacga cgtcgccgaa
     4801 ggggacccca tagcagagat cgagaccgac aaggtcacga tggagctcga agccgaagac
     4861 gcgggaaccc tcgcccagct tatcgccgac gagggccagg atattcccgt tggcgaggcc
     4921 atagctttta ttcagggtga aggtgaagac gtccccgagc gcagcgaagg tggcggagag
     4981 gccgaggaag aggcagaaca gggcggcgaa gacggcggtg gcgcccaggc cacggccacg
     5041 gagaccgagg cccccgagga ggaagagggg g
//
LOCUS       DBNGVD010000095         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3298910, whole genome shotgun sequence.
ACCESSION   DBNGVD010000095 DBNGVD010000000
VERSION     DBNGVD010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3298910"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..2042
                     /locus_tag="AC16CM_02870"
     CDS             372..2042
                     /locus_tag="AC16CM_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR3138799.1"
                     /translation="MAQQSVEEGRSKVGAVFWVSAAVSLAFILWGVIAPTSFGTVTQA
                     VFDWVVSNLGWFYLLAGNFFLVFVFLLALSRYGKFRLGKEGERPEFSTFAWFAMMFQA
                     GMGPSLIFWGVAEPLSHYGAPPFGMAQASTPEAAQLGIQYTYFHWALHPWAMYAVVGL
                     TVGYFSFRRNEPGLISPVFRPLLGDRVDGAWGKTIDVMAVLAVLFGVAVALGTAGLQL
                     TAGLGETFGTPTGIAVQLLVIGVTTVAFMISASTAIEKGINYLSQISMYVAGVLLVFF
                     LVMGPTAVQLGALTQGVGDYFGGIIPMSLRLDSFSPNNSWLGSWTVFYWSWWIAWCPY
                     VGLFIARISRGRTIRQFVLGAVIGPSVVTFVWVAVFGGSALHLAQTQGEGIAARVAAD
                     PASGMFVFLNQFPLAFPMSILTLLVLWIFFVAGADAGTVVLGSMSTGGPQEPKRWIKL
                     SWGVAMAAIAGILLVAGGLGALQSASVLFGLPFAFIMVAMCAAFYMHLRSETRGEDRR
                     EDVPSTPRATGAPSDEGPAPGQAFAAEEPAPGYNRNPGIEKPGTEGRQ"
     gene            2039..3031
                     /locus_tag="AC16CM_02875"
     CDS             2039..3031
                     /locus_tag="AC16CM_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3138800.1"
                     /translation="MIPGRARRSLLGLLVLTIFSAGLVAGCGSGGGSDGVEIAGDQFR
                     LSGTQRDAQFAVGSESFTEQEVLGNIAIQVLEAADATVIDRTGLGGNEEVRRELESGE
                     IDLYWEYTATGWLVHLSETNTIPEPEEQYEAVARQDLEENGIRWLDPAPGNDTYAIAA
                     SQETSEDLGVETISDFKRLVEERPGQATLCLNNDDGFRTRFDGLPGLERAYGFQLPEE
                     NLVETSVDAVYDATDEAEICNFGVVFTTSGFLDELDLELLKDDEDFFAVYNPALTIRQ
                     PVYKQYPQLRKIFVPISQKLDTETLRKLNYSVDVEGKSPENVATNWLRDEGFIE"
BASE COUNT          546 a          945 c          975 g          600 t
ORIGIN      
        1 gacctcatgg tggtagcctg gcggaacgaa acgcgtgacg gccggcccga aagcccagat
       61 gccaacggag ttcttgatca cagggttctc cttcccctcg gatagatacg agactgccct
      121 cccgacaccc atcatagccc gaatcggctt atcatgtggt ttgtagtatc cggtaacggg
      181 ctggtgtagc atacgcccga acgttccgga gccgcttcgc ctgagcgtgg accctcggtc
      241 cgggcgcggc ttacagcgtc tctgccgcga ggttctgtcg caactcgatc aaatgaaggc
      301 cacgcgcata cgtttcagtc accaagcatt gcagctgcta cacaggcaag aggtaaacga
      361 agggagatcg catggcgcag cagagcgtcg aagaaggtag gtccaaggta ggggcggtat
      421 tctgggtgtc ggccgcggtt tcgctggcgt tcattctgtg gggtgtgatc gcaccgacaa
      481 gcttcggcac ggtaacgcag gcggtcttcg actgggtggt ctcgaacctg ggctggttct
      541 acctcctggc gggaaatttc ttcctcgtct tcgtcttcct cctcgccctc agtcgttacg
      601 ggaagttcag gctgggcaag gagggcgaga gacccgagtt cagcactttt gcgtggttcg
      661 ccatgatgtt ccaggccggg atggggccat ccctcatctt ctggggcgtg gcagagcccc
      721 tctcgcacta cggtgccccg cccttcggta tggcgcaagc gtcaactccg gaggcggccc
      781 agttggggat acagtacacg tactttcact gggctctgca cccgtgggcc atgtacgcgg
      841 tcgtaggact tacggtcggg tacttcagct tccgaaggaa cgagcccggt ctcatcagcc
      901 cggtcttcag gccattgctg ggagacaggg tagacggcgc gtgggggaag accatagacg
      961 tgatggcggt ccttgcggtc ctgttcggcg tcgccgtcgc tctgggtact gccggactgc
     1021 agctcaccgc gggactcggc gagaccttcg gcaccccgac cgggatcgcg gtgcaactgc
     1081 tcgtcatcgg cgtcaccacg gtggcgttca tgatctcggc ctccacggcc attgaaaaag
     1141 gtatcaacta cctcagccag atcagcatgt atgtggccgg cgtcttgctc gtcttctttc
     1201 tcgtcatggg ccctacggcc gtccagctcg gcgccctgac gcagggggtc ggcgactact
     1261 ttggcggcat cattcccatg agcctgaggc tcgactcctt ctctcccaac aactcctggc
     1321 tcggaagctg gacggtcttc tactggtcct ggtggatcgc ctggtgtcct tatgtgggcc
     1381 tgtttattgc ccgaatttcc aggggtcgta cgatccggca gttcgtgctc ggggctgtta
     1441 tcggaccgag cgtggttacc ttcgtgtggg tagctgtgtt tggcgggtcg gcccttcacc
     1501 tggcccagac gcagggtgaa ggtatagccg cgagggtcgc cgccgacccg gcctcgggga
     1561 tgttcgtctt cctcaaccag tttccgctcg ccttccccat gtcgatcctg acgctcctcg
     1621 tactctggat cttcttcgtc gccggcgccg acgcggggac cgtggtgctc ggcagtatgt
     1681 ctactggtgg gccccaggag ccgaagcgct ggatcaagct ctcctggggc gtggcgatgg
     1741 ctgctatcgc cgggatactg ctcgtggccg gtggcctggg tgcgctgcag tcggcctccg
     1801 tcctgttcgg cttgcccttt gcttttatta tggtcgccat gtgcgcggcc ttctacatgc
     1861 acttgcgcag cgaaacccgc ggcgaggacc ggcgtgagga cgtaccgtcc acgcctcgcg
     1921 ccacgggggc gccgtctgat gagggtcctg caccaggaca ggcgttcgct gccgaggagc
     1981 cagcacccgg ctacaacagg aaccccggga tcgaaaaacc gggaaccgag gggcggcagt
     2041 gatccctggt cgggcgcgtc ggtcgcttct gggcctgctc gttctcacca tcttctccgc
     2101 tggcctggtc gcgggttgcg ggtcaggcgg aggctcggac ggggtagaga tcgccggcga
     2161 ccagttcagg ctgagcggga cacagaggga cgcccagttt gccgtcggct cggagagctt
     2221 caccgagcag gaggttctgg gcaacatcgc cattcaggtc ctggaggccg ccgacgccac
     2281 ggtgatagac aggacgggcc tcgggggcaa cgaggaagtg cgccgggagc tggaatccgg
     2341 ggagatcgac ctctactggg agtacacggc cacgggctgg ctcgtccacc tctccgaaac
     2401 caacaccata cccgagccgg aggagcagta cgaggcggtg gcccgccagg atctggagga
     2461 gaacggcatc agatggctcg acccggcgcc gggcaacgac acctacgcca tagccgcgag
     2521 ccaggaaacc agcgaggacc tcggcgtaga gaccatctcg gacttcaaac ggcttgtaga
     2581 agaacgtccc gggcaggcta cgctctgcct caacaacgac gacggtttca ggacccgctt
     2641 cgacgggctg cccggcctcg agcgggccta cggcttccag ctaccggaag agaacctcgt
     2701 tgagacatcc gtagacgccg tctacgacgc cactgacgag gcagagatct gcaacttcgg
     2761 tgtggtcttc accacgagcg gcttcctgga cgaactggac ctcgagctcc tcaaagacga
     2821 cgaggacttc ttcgccgtct acaacccggc gcttaccata aggcaacccg tttacaagca
     2881 atacccccaa ctgcgcaaga tcttcgtccc catctcgcaa aaactggata ccgaaaccct
     2941 gcgaaagctc aattactccg tggacgtgga aggcaagtcc cccgagaacg tggctactaa
     3001 ctggctaaga gacgaaggct tcatagaata acggcgggcc acggcgaagg ccggggcgtg
     3061 ctattc
//
LOCUS       DBNGVD010000096         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1295801, whole genome shotgun sequence.
ACCESSION   DBNGVD010000096 DBNGVD010000000
VERSION     DBNGVD010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1295801"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="AC16CM_02880"
     CDS             complement(<1..745)
                     /locus_tag="AC16CM_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138801.1"
                     /translation="MGEWRGEWREANRASWDERVPIHVSGEFYDVAAFKAGEERLQPF
                     EIAEVGDVSGKELLHLQCHFGIDTLSWARRGARVTGLDFSGPAVEAASGLASEMGLEA
                     TFVRSDVYEAAEAVGGRTFDIVYTGRGALNWLPDIGRWAGVVAGLVKPGGFLYLTEFH
                     PFTEIFGDDDLTVEHDYFQDEEPRVWDEPGTYADFEAETVHNVTYEWNHTLGAVISAV
                     IDAGLAVELLHEFDYTKFARWPILEKSGFD"
     gene            complement(839..>2116)
                     /locus_tag="AC16CM_02885"
     CDS             complement(839..>2116)
                     /locus_tag="AC16CM_02885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3138802.1"
                     /translation="GQDESIFQPEWSPDGTLSFVSDRTGWWNLYRLGEEIIEPLCEKE
                     AEFGTPQWGFGLSTYAFLPSGKIFCRYTEQDGSHLAILDPENGNLEPVETPYSSIAFV
                     RADAETGRVVFLGGSPTGPACIVSLDTPTGEHEVLRRSDEVEVDPGYLSIPEPIEFPT
                     ENGKTAYAFFYPPTNRDYAAPEGERPPLLVMSHGGPTAATSTSLDLEIQYWTSRGIAV
                     LDVNYGGSTGYGREYRRRLDGAWGVVDVEDCANGAVYLAERGLVDGERLMITGGSAGG
                     YTTLCALTFTDTFAAGASHFGVSDLEELAKETHKFESRYLDRLIGPYPERADLYRERS
                     PIHHTHKLSCPVIFFQGLEDEVVPKEQAETMFAALREKGLPVAYVPFEGEQHGFRRAE
                     NIRRALDGELYFYSRILGFEPAGQIEPVPIENA"
BASE COUNT          347 a          724 c          664 g          381 t
ORIGIN      
        1 tgtcgaagcc cgatttttcg aggatgggcc accgggcgaa cttcgtgtag tcgaactcgt
       61 gcaacagctc gaccgcgagg ccggcgtcga tcacggcgct tatcaccgct ccgagcgtgt
      121 ggttccactc gtaggtcacg ttgtgtaccg tctcggcctc aaagtcggcg taggtgcccg
      181 gctcgtccca gacccttggt tcttcgtcct gaaagtagtc gtgttccacg gtcaggtcgt
      241 cgtcgccgaa gatctcggtg aagggatgga actccgtcag gtagagaaag ccacccggtt
      301 tgaccagccc agccacgacc ccggcccacc gcccgatgtc gggaagccag ttgagggcgc
      361 cgcggcccgt atagacgata tcgaaggttc ttccccccac ggcctccgcg gcctcgtaga
      421 cgtccgaacg tacgaaggtc gcctccaggc ccatctccga ggccagcccg ctggccgcct
      481 ccacggccgg acctgagaag tccagccccg tcacccgggc gccgcgccgc gcccagctca
      541 gggtgtcgat gccgaaatgg cactgcagat gcaggagctc cttgcccgag acgtcgccca
      601 cctccgctat ctcgaacggc tgcaggcgct cctccccggc cttgaaggcg gccacatcat
      661 agaactcgcc cgagacgtga atcggtaccc gctcgtccca actggccctg ttcgcctccc
      721 gccactcccc acgccactca cccaacgcta gctcctttct tagcggttcg cttcgcgaac
      781 cgcgccgtga gcttcatgtt cttgctgata gctgatagcg cgccgtgcaa gcggcgcttc
      841 aggcgttctc tatcggcacg ggctcgatct ggcccgcagg ttcgaagccg aggatgcgcg
      901 agtagaagta gagctcgcca tccagagccc gcctgatgtt ttcggctcgc ctgaagccgt
      961 gctgctcgcc ctcgaagggc acgtaggcca cgggaagccc cttctcgcgc agcgccgcga
     1021 acatcgtctc ggcctgctcc ttgggcacca cctcgtcctc caggccctga aagaagatca
     1081 ccgggcacga gagcttgtgt gtgtggtgga tcggcgatcg ttccctgtag aggtcggccc
     1141 gctccgggta gggcccgatc aggcggtcca ggtagcgcga ctcgaacttg tgtgtctcct
     1201 tggccagctc ctccaggtcg ctgaccccga aatggctggc gccggcggcg aaggtgtcgg
     1261 tgaacgtgag cgcgcagagc gtcgtgtagc ctcccgcgct gcccccggtg atcatgagcc
     1321 gctcaccgtc cacgagacca cgctccgcca ggtagacggc cccgttggcg cagtcctcga
     1381 cgtctacgac gccccacgcc ccgtcgaggc ggcgccggta ctccctcccg tagcccgtgc
     1441 tcccgccgta gttcacgtcg agcacggcga tgccacggct ggtccagtac tggatctcca
     1501 ggtcaaggga cgtcgaggtc gcggcggtgg gcccaccgtg gctcatcacc agaagcggtg
     1561 ggcgctcgcc ctccggagcc gcatagtccc ggttggtggg cggatagaag aaggcgtaag
     1621 ccgtcttgcc gttctccgtg ggaaactcga tgggctctgg gatggacagg tagcctgggt
     1681 ctacctccac ctcgtccgag cggcgcagga cctcatgctc gccggtgggg gtatccaggc
     1741 tcacgataca cgctggtccc gttggtgaac cgccaaggaa gaccacccta cccgtctccg
     1801 cgtcggcccg cacgaaggcg atgctcgagt aaggcgtctc gacgggctcg aggttcccgt
     1861 tctccgggtc gaggatggcg aggtgcgagc cgtcctgctc ggtgtagcgg cagaagatct
     1921 tcccagacgg gagaaaggcg taggtggata gtccgaaccc ccactgcggg gtgccgaact
     1981 ctgcctcctt ctcgcagagc ggctcgatga tttcttcgcc aaggcggtac aggttccacc
     2041 agcccgtacg gtccgagacg aaagagagcg taccgtcggg ggaccattcc ggctggaaga
     2101 tggactcgtc ttgccc
//
LOCUS       DBNGVD010000097         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3664151, whole genome shotgun sequence.
ACCESSION   DBNGVD010000097 DBNGVD010000000
VERSION     DBNGVD010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3664151"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC16CM_02890"
     CDS             complement(<1..509)
                     /locus_tag="AC16CM_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3138803.1"
                     /translation="MTTDTRNAHPYHMYEAILAQPEAFARVIERNREAVEEFASRTAS
                     CGRIFLAGIGTSHHATLVGEHLMRAYGGGPDVRAVHSFDLALYGPEIRPDDCLVAVSH
                     RGAKRYTALALDEAREKGCPTALITGDAGGGEGRAATVFRTVAQEKSSAHTVSYTAAV
                     AVLASLAGRL"
     gene            complement(543..1160)
                     /locus_tag="AC16CM_02895"
     CDS             complement(543..1160)
                     /locus_tag="AC16CM_02895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3138804.1"
                     /translation="MTVRLHDYLPSGNGYKVRLLLAQLGIPFQRIEYDIASGETRTPE
                     YLENVNANGRVPVLETEDGTLLPESDAILFYLAVGTPYLPEDRLGKARVLQWMFFEQY
                     SHEPNIAVARAWLHVFDVEMTGERRAALEDRQQRGYDALDVMEGHLKSHDYFVGGRYS
                     VADIALYAYTHVADEGGFDLAGYPAVLAWLERVSSQPNYIPISQG"
     gene            complement(1157..1510)
                     /locus_tag="AC16CM_02900"
     CDS             complement(1157..1510)
                     /locus_tag="AC16CM_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138805.1"
                     /translation="MSDERATAGHRGATVAGAMRLLPGPDGERFAKVFGHGSMEVEVY
                     APRGADQQTPHTRDELYVVVSGSGEFVNGPERHHFGPGDVLFVPAGVEHRFEDFTDDL
                     VVWVVFYGPEGGEAG"
     gene            complement(1522..2115)
                     /locus_tag="AC16CM_02905"
     CDS             complement(1522..2115)
                     /locus_tag="AC16CM_02905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138806.1"
                     /translation="MWEGLTGVLEGRVVRLEPLGLGHEEGLFEAAQDERVWRWMHYDA
                     GESREMFHAWLEDALAASEAGTEGAFATVDVVTGKPVGSTRYLALRPEHGVLEIGWTW
                     LASDFWQTGANAEAKLLMLEHAFEDLGCLRVEFKTDARNERSRAALAALPAQFEGVFR
                     KHMLVRGGERRDSAYYSIIDDEWPEVRENLQRRLGVT"
     gene            2261..>3350
                     /gene="pdxR"
                     /locus_tag="AC16CM_02910"
     CDS             2261..>3350
                     /gene="pdxR"
                     /locus_tag="AC16CM_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR3138807.1"
                     /translation="MAKRQVEVPACVSLDASSAEPLHRQLYDGLRSGILAGRLSAGAR
                     LPSTRTLAVELGVSRTTVVAAFDNLLAEGYLEGKVGSGTYVARSLPDEVLGIRARPAS
                     SRPPLSGRPLSRRGRLLANTRTTAVRDTGVPRAFRPGVPALDEFPYGVWRRISGSLWR
                     RPPGALLGYGDPAGYWPLRREISAHLATARAVACDPEQVIVVSGSQQALDLASRVLLD
                     PGDAVWVEDPGYMGARGALAGSGARLVPVPVDGDGLVVAEGIRREPGARLACVTPSHQ
                     YPLGVTMSLARRLELLGWAGRSGSWVIEDDYDAEYRYTGRPLEALQGLDAEGRVVYVG
                     TFSKVLFPALRLGYLVVPPDLTDAFTAAR"
BASE COUNT          519 a         1157 c         1089 g          585 t
ORIGIN      
        1 agccttcccg cgagcgacgc cagcacggcc acggcggccg tgtagctgac ggtatgggcg
       61 gaggatttct cctgcgccac cgtacggaac acggtggcgg cccggccttc gccgccccct
      121 gcatcaccgg ttatgagggc ggtcggacag cccttctccc tggcctcatc tagcgcgagc
      181 gccgtgtagc gcttggcgcc gcgatggctc acggcgacga ggcagtcatc gggccttatc
      241 tctggaccgt agagcgcgag gtcgaaggag tgaacggcgc gaacgtccgg tccgccgccg
      301 taggcacgca tcaggtgctc ccccacgagg gttgcgtggt gcgaggtgcc gatgccggca
      361 agaaagatac gcccgcacga agccgttcgg gacgcgaatt cttcgaccgc ctcccggttg
      421 cgctcgatga ccctggcgaa cgcttcgggt tgggccagga tcgcctcgta catgtggtag
      481 gggtgcgcgt tgcgcgtgtc ggtggtcatg gtcggtcctt tctccgggtc acgcggccct
      541 gttcagccct gtgagatcgg tatgtagttc ggttgtgagg agacccgttc gagccaggcc
      601 agtacggccg ggtaaccggc aagatcaaag ccaccctcgt cggcgacgtg ggtgtaggcg
      661 tacagggcga tatccgccac ggagtagcgc ccgccgacga agtagtcgtg gcttttcaga
      721 tgcccctcca tgacgtcgag cgcgtcgtac ccgcgctgct gcctgtcctc cagtgcggcc
      781 ctgcgctccc cggtcatctc cacgtcgaaa acgtggagcc aggcgcgggc cactgcgatg
      841 ttgggctcgt ggctatactg ctcgaagaac atccactgca agacccgcgc cttgccgaga
      901 cggtcctccg gaaggtacgg ggtgcctact gcgagataga acaggatggc gtcggactcg
      961 ggcaggagcg tgccgtcctc cgtttcgagc acgggaacac gaccgttggc gttgacgttt
     1021 tcgagatact ccggcgtgcg cgtctcgccg ctggcgatat cgtactcgat cctctggaac
     1081 gggatgccga gctgcgcgag caacagcctg accttgtagc cgttgcccga gggcagataa
     1141 tcgtgcagcc gcacggtcat ccggcctccc cgccctcagg gccgtagaag acaacccaca
     1201 ccacgagatc gtcggtaaag tcctcgaagc ggtgctcgac gccggctggc acgaagagca
     1261 cgtcgcctgg cccgaaatga tgccgttccg gcccgttgac gaactcgccg ctcccgctga
     1321 cgaccacgta cagctcgtcg cgggtgtgcg gcgtctgctg atccgcgccc cgcggtgcgt
     1381 agacctcgac ctccatgctc ccatgcccga acaccttcgc gaaccgctct ccgtcaggtc
     1441 ccggcaacag ccgcatggcc ccagcaacgg tagctccccg atgcccggcc gtggctcttt
     1501 catcgctcat ccggcctccc ttcatgtgac ccccagcctc cgctggaggt tctctctgac
     1561 ctcgggccat tcgtcgtcga tgatgctgta gtaggccgag tcgcggcgtt cgccgccgcg
     1621 cacgagcatg tgcttgcgga agacgccctc gaactgggcc ggcagggccg ccagggccgc
     1681 acgcgagcgc tcgttgcggg cgtccgtctt gaactcgacc cgcaggcacc cgagatcctc
     1741 gaacgcgtgc tcaagcatca ggagctttgc ctcggcgttc gcgccggtct gccagaaatc
     1801 ggacgccagc caggtccacc cgatctccaa caccccgtgt tcgggtcgca aggccagata
     1861 ccgcgtgctc cccacgggct tccccgtcac cacgtccacg gtggcaaagg caccctccgt
     1921 accggcctca gaggcggcca gggcatcttc cagccaggcg tgaaacatct ccctggactc
     1981 gcctgcgtca taatgcatcc agcgccagac cctctcgtcc tgcgcagcct cgaagagccc
     2041 ctcctcatga cccagaccca acggctccag ccgcacgacc ctcccctcca gaacgccggt
     2101 caaaccctcc cacatgccac ccatcctcct gtcgaaacca gttggaaaac atgttaacgg
     2161 aatatggtcc ctgcgccaga tccattatca tgtcagcaga ccagaccatt caggctgaag
     2221 gctgatagct aatagctgac agctaccaag gaagaggagc atggccaaga ggcaggtgga
     2281 ggtaccggca tgtgtctcgc ttgacgcttc ttcggcggag ccgcttcacc ggcagctcta
     2341 cgacgggctg cggtcgggta ttctggccgg gcggctttcg gcgggggctc gcctgccctc
     2401 tacgcgcacg ctcgccgtcg agctcggcgt atcccgcacc accgtggttg cggccttcga
     2461 caatctgctc gccgaggggt atctggaggg gaaggtcggg tcgggcacct acgtcgcccg
     2521 ttctttaccc gacgaggtgc tcggcatccg ggcgaggccc gcctcctccc gtcccccgct
     2581 ctccgggcgg cctctctccc ggcgcgggag gttgctcgcg aacacgcgta ccaccgcggt
     2641 cagggacacg ggggtgccgc gtgcgtttcg gcccggcgtg ccggcactag acgagtttcc
     2701 ctacggggtg tggcgccgga tctccggtag cctgtggcga cggccgccgg gggcgctgct
     2761 cggttacggg gacccggccg ggtactggcc gctgcggcgg gagatctccg cccacctcgc
     2821 gacggcgcgg gccgtggcgt gcgaccccga acaggttatc gttgtctccg gctcgcagca
     2881 ggcactcgac ctcgcttctc gtgtgctcct ggaccccggc gacgccgtgt gggtcgagga
     2941 ccccgggtac atgggcgcaa ggggggcgct cgccgggtcc ggcgccaggc tcgtgcccgt
     3001 ccccgtcgat ggagatggcc tggtggtggc ggaaggtatc cggcgcgagc cgggggcacg
     3061 tctggcgtgc gtgacgccct cgcaccagta cccactcggc gtcaccatga gcctcgcccg
     3121 tcgcctggag ttactggggt gggcgggacg ttccggttcg tgggtgatag aagacgacta
     3181 cgacgcagag taccgttaca cggggcgccc gctcgaggcc ctgcaggggt tggacgcgga
     3241 gggccgcgtg gtctacgtgg ggaccttcag caaggtcctc tttccagccc tccggctcgg
     3301 ctatctggtt gtgccaccag atctcacgga cgccttcacg gcagcccgcg
//
LOCUS       DBNGVD010000098         2080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4756759, whole genome shotgun sequence.
ACCESSION   DBNGVD010000098 DBNGVD010000000
VERSION     DBNGVD010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4756759"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC16CM_02915"
     CDS             complement(<1..360)
                     /locus_tag="AC16CM_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3138808.1"
                     /translation="MSESVHKHRESAPDSVRVAVLTISDTRTPETDTGGDVAEELLVG
                     AGQEVVERRIVRDEASGIRNSLVDLLASSEVDAVVTTGGTGISGRDTTYEVVERMLEK
                     RLDGFGEIFRMLSYEEIG"
     gene            complement(357..995)
                     /locus_tag="AC16CM_02920"
     CDS             complement(357..995)
                     /locus_tag="AC16CM_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3138809.1"
                     /translation="MILKMLTVGPFQENCYIIGDEESGVGALVDPGDEAARIAMAVEE
                     TNLDIGSIIVTHAHIDHVGGVVSLSEEYSCPVLMHDESQPLLEGLPTQAMMMGIRFGK
                     VPTVDRYVGDEEILEVGGLRLRSLYTPGHAPGHLAFYVEDEGLVLSGDALFAGSVGRV
                     DLPGGSMEVLMRSIEERLLTLPDETVVYPGHGPRTTIGDERTANPFLQGGMF"
     gene            complement(992..>2080)
                     /locus_tag="AC16CM_02925"
     CDS             complement(992..>2080)
                     /locus_tag="AC16CM_02925"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3138810.1"
                     /translation="AGLGLYRFVKHKSAADVHELEHFWLVVEEGELEAARAGAEIGEK
                     VAGGACLARDLANEPSNVATPDYLAERAREIAERHGMDLRILDRANIEAEGLTGLATV
                     GRSASNEPRFIVLEHRKGGGSAPVVLVGKAVTFDSGGISIKPSAGMEDMKFDMSGGAA
                     VLGAMEAVGAMGLPLDVVALVPATENLPGGDAFKPGDVLQMQSGKTVEIVTTDAEGRL
                     ILADALTYASRYEPSAVIDCATLTGACVVALGSHASGLMGNDDDLISEVQEAGETAGE
                     RAWPLPLFGEYTEQIKGDTADIKNSGGRGGGALTAGAFLKEFADYPWAHLDIAGTAYG
                     KKSNAYTTRGGTGVPTRLLVEFLIGRSS"
BASE COUNT          340 a          732 c          629 g          379 t
ORIGIN      
        1 gccgatctct tcgtaggaga gcatcctgaa gatctcgccg aacccgtcga gcctcttctc
       61 gagcatgcgc tccacgacct cgtaggtggt gtcgcggccc gagatgccgg tgccgcccgt
      121 cgtgacgacg gcgtccacct ccgaactcgc gagcaggtca acgaggctgt tccttatgcc
      181 ggaggcctca tccctgacga tccggcgctc cacgacctcc tgcccggcac cgacaagcag
      241 ctcctccgcg acgtcgccgc cagtatcggt ctccggcgtc ctggtgtcgc tgatggttag
      301 cacggccacc ctgacagaat caggtgccga ctccctgtgc ttgtggacgc tctcgctcaa
      361 aacatgcccc cttgcaggaa cgggttggcg gtccgctcgt ccccgatggt ggtccgtggc
      421 ccgtggcccg ggtacacgac cgtctcgtcg ggaagcgtca acagccgttc ctcgatgctc
      481 cgcatcagca cctccatgct gccaccgggt aggtcaaccc ttcccacgct accggcaaag
      541 agcgcatccc cggacagcac gagcccctcg tcctcgacgt agaaggcgag gtgaccaggc
      601 gcgtggccag gagtgtaaag cgagcgcaac cggagacccc cgacctccag gatctcttcg
      661 tctcccacgt agcggtcgac ggtcggcacc ttgccgaacc tgatgcccat catcatggcc
      721 tgggtaggga gcccttctag caacggctgt gattcatcgt gcatcagcac gggacaggag
      781 tactcttcgg agagggagac cacccctccc acgtggtcga tgtgggcgtg ggttacgatg
      841 atcgacccga tatcgaggtt cgtctcctcg acggccatgg ctatccgggc cgcctcatcg
      901 ccggggtcga ccagcgcccc gaccccggat tcctcatcgc cgatgatgta gcagttctcc
      961 tggaacgggc caacggtcaa catcttcagg atcatgacga cctcccgatc aggaactcga
     1021 ccagcagccg ggtcgggacg ccggtcccac ccctggtagt gtacgcgttg cttttcttgc
     1081 cgtaggccgt tcccgctatg tcgaggtggg cccacgggta gtcggcgaac tctttcagaa
     1141 acgctcccgc tgttagcgcg ccacccccgc gcccgcccga gttctttatg tcggccgtat
     1201 cgcctttgat ctgctcggtg tactccccga agaggggtag gggccaggcc ctctcgccgg
     1261 ccgtttcgcc cgcttcctgc acctcagaga tcaaatcgtc gtcgttgccc atgaggcccg
     1321 aggcgtggct acccaacgcc accacacagg cgccggtgag ggtagcgcag tcgattacgg
     1381 ccgagggctc gtagcggctg gcgtaagtga gggcgtcggc caggatgagg cgtccctctg
     1441 cgtcggtggt gacgatctcg accgtcttgc cagattgcat ctggagcacg tccccgggct
     1501 tgaaggcgtc cccaccggga aggttctcgg tcgccggaac cagcgcgacg acgtcgaggg
     1561 gcaggcccat ggccccgacc gcctccatcg ccccgagcac cgccgccccg ccgctcatgt
     1621 cgaacttcat gtcctccatg ccagccgagg gctttatgga gatgcccccg gagtcgaagg
     1681 ttacggcttt accaacgagg acgacgggag ccgacccgcc ccccttgcgg tgttccagca
     1741 cgataaagcg cggttcgttt gacgccgacc ggcccaccgt ggcaaggcca gtaagcccct
     1801 cggcctcgat gttagctcgg tccagaatgc ggaggtccat gccgtgcctc tcggctatct
     1861 cacgcgcccg ctccgccagg taatcaggag tggccacgtt ggagggctcg tttgcgaggt
     1921 cgcgggccag acaggcgccc cccgctactt tctccccgat ctcggccccg gcgcgagccg
     1981 cctccagttc tccctcttcg acgaccagcc agaagtgctc gagctcatgg acgtcggccg
     2041 cgctcttgtg cttgacgaag cggtacaggc cgagaccagc
//
LOCUS       DBNGVD010000099         3379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3117896, whole genome shotgun sequence.
ACCESSION   DBNGVD010000099 DBNGVD010000000
VERSION     DBNGVD010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3117896"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16CM_02930"
     CDS             <1..615
                     /locus_tag="AC16CM_02930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3138811.1"
                     /translation="RAATIQGDLSNTAECEKTVDAAAEALGGLDALINNAGITKTADF
                     LDTDEELYDSVFDLNMKGYFFLARKAVPFMMERGGGSIVNITSVHGFAGFPQHTAYAA
                     TKGAINGFTRQLAIELTDRKVRVNAVGPGIIEVPRYFTDNPGYTTEFGNTLVPWGRVG
                     HPEDVGPTVAFLVSDAADFVTGQVLYVDGGTTARMGLWWGQGRE"
     gene            705..1790
                     /locus_tag="AC16CM_02935"
     CDS             705..1790
                     /locus_tag="AC16CM_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3138812.1"
                     /translation="MSERGSGLGSRKLGRKQFLAGSALASAALMLAGCRQDAAQQAGG
                     GGGSGGNFPDTPEWNFVFINHVTTNPFFVPTRYGIEDAQALLGTTSQWTGSENSVVKD
                     MVDSMDTAISGNANGIAVSVVDPVAFNKPIQKALDNGIPVIAYNANGKGPGTNPALAY
                     VGQDLFQSGIEMGKRIVSLVDEGLVALFIATPGQLNIQPRIDGAKQAIEDSGANIEIQ
                     QVTTGAELPDELSRIEAWYNGHKDVAGMFAVDAGSTQGVAQVMEKFGLHEQGVRAGGY
                     DLLPQILKLMKAGHIDFTIDQQPYLQGFFPVMQLYLYNISGGVTGPAETNTGLIFVTP
                     DTVDRYLETESRFEGDSETQKIVKAPS"
     gene            1828..2856
                     /locus_tag="AC16CM_02940"
     CDS             1828..2856
                     /locus_tag="AC16CM_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138813.1"
                     /translation="MSETTRESAAGDTAGAGTQESGLVGRLLTGLVRVREVSILVVLV
                     GLALYFQAANSAFLSPQNLLSLSQYAVPYVVIASALCLLLICGEIDLSVGQSFAFAPI
                     VMYLAYDKLFLVLPLAVIVGLLAVAGVGLINGLITVYLGVSSLITTLGMFFLLAGLNV
                     TLTGGFPATTPESSSVTRALGEGAWSGTVWAILIIGLLQLVLSRTRWGLHTYATGGNP
                     LGSREAGVSIARIKIGNFMLSGMLGGFVGIIESFRIQSIDPLLGGPDIVLLCIASAVI
                     GGTALLGGVGTVTGAFLGALVLTVLRNGFTLQGIGAYTFNIILGSAILVAMILNVYVA
                     RLRGAGRS"
     gene            2856..>3379
                     /locus_tag="AC16CM_02945"
     CDS             2856..>3379
                     /locus_tag="AC16CM_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3138814.1"
                     /translation="MENGSTNGTANGQDFLRVEDLVKRFGAISVLKGVDLHVKKGEVL
                     GLIGDNGAGKSTLVKTITGFHRPDDGRILIDGDEVNLRSVAQARSLGIQTVYQDLALV
                     NELSVFHNMFLNGELTIGPFLNNRKMKEKTSQYLADMGVNIPSIDTEVAMLSGGQRQA
                     IAVARAVYSDARMLL"
BASE COUNT          633 a         1095 c         1071 g          580 t
ORIGIN      
        1 cgggccgcca ctattcaggg cgacctgagc aacaccgcgg agtgcgagaa gaccgtggac
       61 gccgccgcag aggcgctcgg cgggctcgac gccctgatca acaacgcagg catcacgaag
      121 acggccgact tcctcgacac ggacgaggag ctctacgact ccgtcttcga cctgaacatg
      181 aagggttact tctttctcgc caggaaggcc gtgcctttta tgatggagag gggtgggggg
      241 agcatcgtca acatcacctc cgttcacggg ttcgccggct tcccccagca cacggcctac
      301 gccgcgacca agggggccat caacggcttc acccgccaac tggccataga gctcacggac
      361 cgaaaggtcc gcgtcaacgc cgttggccca ggcatcatcg aggtcccccg ctacttcacc
      421 gacaaccccg gctacaccac cgagttcggc aacaccctgg ttccgtgggg ccgcgtgggc
      481 caccccgaag acgttggccc aaccgtcgcc ttcctcgtct ccgacgcggc ggacttcgtg
      541 accgggcagg tcctctacgt ggacggtggt accacggcca ggatgggcct ctggtggggg
      601 cagggcaggg agtagatccc gaactcttga cacgaatacc gagtggtatg acaatgtcag
      661 acaggggaac agggcgtcgg caaagccaaa gacgggggta gtgaatgagt gagcgtggat
      721 ccgggctcgg cagccgcaag ctgggccgca aacagtttct ggccgggagt gcgctggcat
      781 cggcggcgtt gatgctggca gggtgccggc aggatgctgc gcagcaggcg ggcggtgggg
      841 gtggatccgg cggcaacttc cccgatacgc cggagtggaa cttcgtcttc atcaaccacg
      901 tgaccaccaa ccccttcttc gtaccgacca ggtacggcat cgaggacgcg caggcgctcc
      961 tcggcacgac ctcgcagtgg accggctcgg agaactcggt ggtcaaggac atggtcgatt
     1021 ccatggacac cgccatctcc ggcaacgcca acggcatcgc cgtctccgtg gtggaccccg
     1081 tagccttcaa caagcccatc cagaaggccc tggacaacgg catccccgtc atcgcctaca
     1141 acgccaacgg caaaggccct ggcacaaacc cggccctggc ttacgtgggg caggacctct
     1201 tccagtcagg catagagatg ggcaagcgga tcgtcagcct cgtcgacgag gggctcgtgg
     1261 cgctcttcat agcgacgccg gggcagctca acatccagcc gaggatcgac ggtgccaagc
     1321 aggccataga agactccggg gccaacatcg agatccagca ggtcaccacg ggcgcggagc
     1381 tgcccgacga gctgtccagg atcgaggcct ggtacaacgg ccacaaggac gtggccggga
     1441 tgttcgccgt cgacgccggg agcacgcagg gcgtcgccca ggtcatggag aagttcgggc
     1501 ttcacgaaca gggcgtcagg gccggcggct acgacctcct gccccagatc ctcaagctca
     1561 tgaaggctgg gcacatagac ttcacgatcg accagcagcc ctacctgcag ggtttcttcc
     1621 ccgtcatgca gctgtacctg tacaacatct cgggcggcgt gacgggacca gccgagacga
     1681 acacaggcct catcttcgtc acgcccgaca cggtggaccg ttacctcgag accgagtcgc
     1741 gcttcgaggg cgactccgaa acgcagaaga tagtgaaggc gccttcctag gccagggagt
     1801 tctcgcggag agcgggagga ggaaaacttg agcgagacca cacgcgagag cgccgcgggc
     1861 gacacggcgg gcgcgggaac gcaggaaagc ggtctggtcg gcaggctgct aaccggtctc
     1921 gtcagggtga gggaggtgag catactcgtc gtcctggtgg ggctggcact ctacttccag
     1981 gcggcgaaca gcgcgttcct atccccgcag aaccttctct cgctgtccca gtacgccgtc
     2041 ccttacgtgg tcatcgcgtc ggcgctttgc ttgctcctga tctgcggtga gatcgacctc
     2101 tccgtcggcc agtctttcgc gttcgcgcct attgtgatgt acctggccta cgataagctg
     2161 ttcctggtcc tgccgctagc cgtcatcgtg gggcttctgg cggtggcggg ggtcggcctg
     2221 atcaacggcc tcatcaccgt atacctcggc gtctcatccc tgatcacgac gcttggcatg
     2281 ttcttcctgc tcgccgggct gaacgtaacg ctcacaggcg gcttccccgc gaccacaccg
     2341 gagtcgagct ccgtaacccg ggcgctcggg gagggtgcct ggtcgggcac cgtgtgggcc
     2401 atcctcatca taggcctcct gcagctcgtc ctctcgcgca ccagatgggg ccttcacacc
     2461 tacgccacag gcggcaaccc cctcgggtcc agggaggcgg gggtgagcat cgcgcggatc
     2521 aagatcggca acttcatgct aagcggcatg ctcggcggtt tcgtcggcat catcgagtcc
     2581 ttccgcatac agtctattga tccgctcctc ggcggacccg acatagtgct cctgtgcatc
     2641 gcatcggcgg tcataggagg cacggcgctg cttggtggcg tcggaacggt tacaggcgcg
     2701 ttccttgggg cgctggtgct caccgtgttg cgcaacggct tcaccctgca gggtatcggc
     2761 gcgtatacgt tcaacatcat cctgggctcc gccatcctcg tggccatgat cctcaacgtc
     2821 tacgtcgcga ggctgcgcgg cgcggggagg tcgtgatgga gaacggttct accaacggca
     2881 ccgccaacgg ccaggacttt ctgcgcgtgg aggacctggt caagcgcttc ggggccatct
     2941 ccgtcctcaa gggcgtggat ctgcacgtca agaaaggcga ggtgctcggg ctcatcgggg
     3001 acaacggggc cggcaagagc acgcttgtca agacgataac aggcttccac cgtcccgacg
     3061 atggaagaat cctgatcgac ggcgacgagg tcaacctgcg ctcggtcgcc caggcccgct
     3121 ctcttggcat ccagaccgtc taccaggatc tcgccctcgt caacgagctc tcggtgttcc
     3181 acaacatgtt cttgaacggc gagttgacca tcgggccgtt cctcaacaac cgcaagatga
     3241 aagagaagac ctcccagtac ctcgcggaca tgggcgtcaa catcccttcc atagacaccg
     3301 aggtggcgat gctctcgggt gggcagcgcc aggccatagc cgtcgcgcgc gcggtctact
     3361 cggacgccag gatgctcct
//
LOCUS       DBNGVD010000100         2981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_385782, whole genome shotgun sequence.
ACCESSION   DBNGVD010000100 DBNGVD010000000
VERSION     DBNGVD010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_385782"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC16CM_02950"
     CDS             complement(<1..522)
                     /locus_tag="AC16CM_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3138815.1"
                     /translation="MATATEHERVREMDEPNNTIIRPNRILLATDGSEDARLAGRAAA
                     DLAARTRADLHVAHAWRHHITGLGYPSVAWTDYSYLDEREARRILASEVDAIEASGVT
                     VAEPHLLQGPPIDAILDLCDELRPGLLVLGSRGLGPIGRILLGSVSEGIIHHARCPVL
                     VIRGGEGAWPPAKV"
     gene            complement(529..1236)
                     /locus_tag="AC16CM_02955"
     CDS             complement(529..1236)
                     /locus_tag="AC16CM_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3138816.1"
                     /translation="MELSTSTRQKPDTATMAGLPGFLEGVASAITADAVFIVDPECHI
                     VYWDARAESLTGLLAEEMVDRHCYEVLSGEGEDGTSFCARVCSAVRLARAGQPANDYE
                     VRVFTRSGGERWVGVSSLAVPTDRGPYLVGLMRDVRAAHETLEMARYVVRHSRQDTRE
                     KRAVRRDRDAPALTPRQAEILGLLASGKSAGEIGRELYLSQATVRNHIRALLLALGAH
                     SQLEALARAREAGLLDG"
     gene            complement(1440..2327)
                     /locus_tag="AC16CM_02960"
     CDS             complement(1440..2327)
                     /locus_tag="AC16CM_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3138817.1"
                     /translation="MAMFPTKVLLATDGSAQAQRAAEMAAELSGRHGAELHVVYVEPV
                     PEAYVNAWSSADAEFSELVRGQAEKDAQAKADRESAKIKETGHEVTRAHGKMGQPEAE
                     IVRLAEEIGAGLTVVGSRGLGPLRRALIGSVSNGVVRHAHSSVLVVRGSGRLPGKILL
                     ANDGSEGAESASEVAAEIAATTGSELTILVALSLVTPYPSRLAQDTWGNTKHQARTFA
                     EQEATRLSAETSTDVEGRLVLGRPDVEIVRAGEDLGADMIVVGSRGIGAVRRAVMGSV
                     SDSVVRHAHCPVLVVRPER"
     gene            complement(2340..2558)
                     /locus_tag="AC16CM_02965"
     CDS             complement(2340..2558)
                     /locus_tag="AC16CM_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138818.1"
                     /translation="MDLAHGDQALRQPLHYVSGTASAESERDGYEKLTGGELLHLFAA
                     FRRLRRYRGQAVVPPLMRWGRWVATGIG"
BASE COUNT          476 a         1035 c          931 g          539 t
ORIGIN      
        1 aaccttcgcc ggcggccagg caccctcgcc gcctcggata acgaggaccg ggcaccgcgc
       61 gtggtggatg atgccctcgg agacacttcc gagcaggatg cgtccgatgg gaccgaggcc
      121 acggctaccc agaaccagca gtcccggccg cagttcgtcg cagaggtcga ggatggcgtc
      181 tatcggtgga ccctgcagga ggtggggctc ggcgacggtg acgccggagg cctcgatcgc
      241 gtccacctcg gaggccagta ttctcctcgc ctcccgctcg tcgagatagg agtagtcggt
      301 ccaggccaca gagggatagc cgagccctgt gatgtggtgg cgccacgcgt gggcgacgtg
      361 cagatcggcc ctcgtcctgg cggccagatc cgcggccgcg cggccggcga gcctcgcgtc
      421 ctccgagcca tccgtggcca acagtattct gttcggccgt atgatcgtgt tgttcggttc
      481 gtccatctcc ctgacccttt catgctccgt ggccgtggcc acccatgcct atccatccag
      541 caggccagcc tcgcgcgccc gggcgagagc ctcgagttgc gagtgggctc cgagcgccag
      601 aaggagcgcc cttatgtggt tgcgtaccgt ggcctgcgag aggtagagct ctcggccgat
      661 ctcgcccgcc gacttgccgg atgcaagcag cccgaggatc tcggcctgcc ggggtgtgag
      721 cgccggagcg tccctatctc ggcgcacggc acgcttctct cgggtgtcct ggcgtgagtg
      781 cctgaccacg tagcgggcca tctcgagcgt ctcgtgggct gcccgcacat cgcgcatcag
      841 gccaaccaga tagggtcccc tatcggtagg cacggcgagg ctgctcacgc cgacccaacg
      901 ctcccctcct gaccgggtga acacacgcac ctcgtagtcg ttggcgggct gcccggccct
      961 ggcgagcctg acggccgagc acacgcgggc gcagaaggag gttccgtcct caccctcgcc
     1021 gctcagtacc tcgtagcagt gtctatcgac catctcttcg gcgaggagcc cggtcaacga
     1081 ctcggccctg gcgtcccagt agacgatgtg gcactccggg tccacgatga agacggcgtc
     1141 cgccgtgatg gcgctcgcca cgccttcgag gaacccggga agccctgcca tcgtggcggt
     1201 gtcgggcttc tgtctcgtac tcgtggagag ctccataacc ccgtcccttc tcctcggttg
     1261 ccgcggcctt ctcatgaacc ttatgatggt ccggaccggg gtccgcgtcg tgggcagggc
     1321 taaccatctt cggcagggac ttgcgcctac agggagctgg gcaaaattac ccacgctgct
     1381 tcaaaggccc cggtagccgg ggtcgcctgc ggaggaagcg tcgaagtaaa gcttcgccgc
     1441 tagcgctctg gtcgaaccac gagaacgggg cagtgggcgt ggcgcaccac cgaatccgat
     1501 acgctcccca tcacggcacg cctcacggcc ccgatacccc ggctacccac cactatcatg
     1561 tcggcgccca gatcctcgcc cgcccgtact atctctacat cgggcctgcc gaggacgaga
     1621 cgcccctcga cgtccgtgct cgtctcggcg ctcaggcggg ttgcctcctg ttcggcgaag
     1681 gtgcgggcct gatgcttggt gttcccccac gtgtcctggg caagacggga ggggtagggc
     1741 gtgaccagcg acagggcgac gagtatggtg agctccgatc cggtcgtggc cgcgatctcg
     1801 gccgccacct ccgaggcgga ctcggctccc tcggagccgt cgttggcgag caggatcttg
     1861 ccgggtagtc gaccactacc ccggaccacc agcacggagc tgtgcgcgtg gcgaacgacg
     1921 ccgttcgaga cgctaccgat aagggcgcgc ctcaagggtc ctagcccgcg gctgccgacg
     1981 accgtaagcc cggccccgat ctcctcggct agccgcacga tctcggcctc aggttgtccc
     2041 atcttgccgt gggcacgcgt cacttcgtgg ccggtttcct ttatcttcgc cgattcccga
     2101 tcggccttcg cctgcgcatc tttctccgct tgcccgcgaa cgagttcgga gaactcggcg
     2161 tctgcagagg accacgcgtt gacgtatgcc tcgggcacgg gctccacgta gaccacgtgc
     2221 agctccgcgc cgtgcctgcc ggagagctcc gcggccatct ccgcggcccg ctgagcttgc
     2281 gcggacccat ctgtcgccag caacaccttc gtcggaaaca tcgccatcgc ttcctcctct
     2341 catcctatcc ccgtcgccac ccaccggccc catctcatca ggggtggcac tacggcctga
     2401 ccgcggtagc gcctcaacct gcgaaacgcc gcgaacaggt gcagcaactc tccgccggtc
     2461 agcttctcgt agccatcccg ctcgctctct gccgacgctg taccagaaac gtaatgcagg
     2521 ggctgccgca gggcctgatc accatgggcc aagtccatga tcaaatctag ccaggttttc
     2581 ctctcgggcc gccacctgtt ggagacctct gctccacatc tgatgtggag cagaggttct
     2641 gagctgcacg ccccaactat cggcgtgtta gaatttttgg gcaggaaagg gccgccaggc
     2701 agcgttcctc ggagatgtct tcaacgtgac gatccttgac gtgcaaagag cgtctactac
     2761 ttacgaacgg tcttgtcgtg ccgccgaacg ggccccgtct gctccaggtt ccgctgcagg
     2821 gctctgccgg tgagcgaacg cctggacaga ggatggcccg agcgccgggc tgaggataga
     2881 aacgacaccc ccgaccaggt gacgtccatt ctcgagagca tctccgatgg tttcttcgcc
     2941 gtcgatgagg actggcgatt tacttacgtg aacgccaggg c
//
LOCUS       DBNGVD010000101         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3482961, whole genome shotgun sequence.
ACCESSION   DBNGVD010000101 DBNGVD010000000
VERSION     DBNGVD010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3482961"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16CM_02970"
     CDS             <1..291
                     /locus_tag="AC16CM_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HIR3138819.1"
                     /translation="RLSNAYMDMLGKLAAHHMETGQYRESIRGCYKVLEKELCHEGSY
                     RLLMHCYARLGLRGRALEQYRICEKILEHKYGTTPSSETRAVYASILNHRSV"
     gene            403..612
                     /locus_tag="AC16CM_02975"
     CDS             403..612
                     /locus_tag="AC16CM_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138820.1"
                     /translation="MNDAVRDRLVTILAAGIAYLISQQVVDRLIDIPDEPGLRDDVIE
                     AVLKGATTATSTILASIIVRRLLKG"
     gene            690..>1520
                     /locus_tag="AC16CM_02980"
     CDS             690..>1520
                     /locus_tag="AC16CM_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3138821.1"
                     /translation="MRVVVIGGSGHVGTFLVPRLVEAGHEVVSVSRGTRDPYQANPAW
                     RSVEQVTLDREAEEARGTFGERVLELEPDAVVDMICFTPESAEQLVEALRGRIRHLLH
                     CGTIWVHGHSTQVPATEDLPRFPFGDYGVQKAAIEAYLLDETRKGGIPATILHPGHIV
                     GPGWAPLNPAGNFDLGVFERLARGSELAVPNLGMETVHHVHADDVARAFVLALQNWSV
                     AVGESFHVVSPAALTLRGYAEAVSGWFGQTADLRFVPWERWREEVSEEEAAGTWDHIA
                     H"
BASE COUNT          287 a          454 c          515 g          264 t
ORIGIN      
        1 cgcctctcca acgcctacat ggacatgctg ggcaaactcg ccgcgcacca catggagacc
       61 ggccagtatc gggagagcat caggggatgt tataaggttc ttgagaagga gctttgccac
      121 gagggcagct accgccttct gatgcattgc tacgcgaggc taggcctgag gggaagggcg
      181 ctggagcagt acaggatctg cgagaagatc ctcgaacata aatacggtac gacgccctcc
      241 tccgagacgc gggccgtcta cgcgagcatc ttgaatcaca ggagcgtgta gctcagacct
      301 ccgactcttg tgtggtgaag gttcgaaacg tgctttcgtg tttccgagta gtctggcctg
      361 ggtagttatt cgggcagaat gcaggagacc gaaggaacgg ttatgaacga cgcggtgcgg
      421 gatcggctgg taaccattct ggcggcgggg atcgcctatc tgatcagcca gcaggtcgtc
      481 gacaggctta tagatatacc cgacgagccc gggctcaggg acgatgtcat agaggccgtt
      541 ctcaaggggg ctaccaccgc gacatcgacc atcctcgcct ccataatcgt gcggcggttg
      601 ctcaagggct gaaccgcgcg agacgccatg ttcgccaggg gccacggaca tcaacaccgc
      661 ggttccggcc tacgccagga gggtatctga tgcgcgtcgt ggtgataggg ggcagcgggc
      721 atgttggtac cttcctcgtg ccccggctgg tcgaggccgg gcacgaggtg gtctctgtca
      781 gtcgtggcac gagggatccc taccaggcca accccgcctg gcgctccgtc gagcaggtaa
      841 cgttggatcg cgaggccgaa gaggccagag gcaccttcgg ggagcgggtg ctcgagctgg
      901 agcccgacgc ggtcgtggat atgatctgtt ttacgcccga gagcgccgaa cagctcgtcg
      961 aggcactgcg gggcaggatc aggcatcttc tgcactgcgg gaccatctgg gtacacggac
     1021 acagcacgca ggtgccagcg accgaggacc ttccccgctt tcctttcggc gattacggcg
     1081 tgcagaaggc tgctatcgaa gcctaccttc tggacgagac gcggaagggc ggcatacccg
     1141 cgacgatcct gcaccccggg cacatcgtgg gtccaggctg ggcgcccttg aacccggcgg
     1201 gaaacttcga cctcggggtc ttcgaacgac tggcaagggg aagcgagctg gccgtcccca
     1261 acctcggcat ggagaccgtg caccacgtcc acgccgacga cgtggcccgg gcgttcgtcc
     1321 tggccctcca gaactggagc gtcgctgtgg gagagagctt ccacgtggtc tctccggcag
     1381 ctctgaccct gcgaggatac gccgaggcgg tctccggctg gttcgggcag acggcggacc
     1441 tccgcttcgt gccgtgggag cggtggcgcg aggaggtctc cgaagaggaa gccgcgggga
     1501 cctgggacca catcgcccac
//
LOCUS       DBNGVD010000102         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2390138, whole genome shotgun sequence.
ACCESSION   DBNGVD010000102 DBNGVD010000000
VERSION     DBNGVD010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2390138"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="AC16CM_02985"
     CDS             complement(<1..1175)
                     /locus_tag="AC16CM_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HIR3138822.1"
                     /translation="MGVPREGAISFLARDRTIAGPGFNRWLIPPAALLVHLSIGEIYA
                     FSVFKTPLVERFGTGLTPIAIIFSISIVMLGLSAAVFGTWVERAGPRAAMFTAALCWS
                     SGFFISALGVATSQLWLVYLGYGLIGGIGLGIGYISPVSTLIKWFPDRPGLATGLAIM
                     GFGGGALIASPLTDRLLTAYASNPVDAIVPSFLTLGVIYLIAMMIGAFVVRLPAEDWQ
                     PEGWTPPTEEETSALETTANVSADSAIRTPQFWLLWTVLFCNVTAGIGLLEQAAPMIQ
                     DFFAVTAAAAAGFVGLLSLANMAGRLGWSSTSDYIGRKPIYMVYLGVGTVMYFVLASF
                     GSASLAMFLVLTLVILSFYGGGFATIPAYLKDLFGVAQVGAIHGRLLTAWSAAGVAGP
                     "
     gene            1420..>3260
                     /locus_tag="AC16CM_02990"
     CDS             1420..>3260
                     /locus_tag="AC16CM_02990"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1867..1869,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3138823.1"
                     /translation="MATAIEKVSENGKADGAREVATICGFCGVGCGLILKVEDGVIQK
                     VTGEKENPSSRGEACIKGREGWRHLYSDRRITRPLIRKDGELVPASWDEALDLVASKM
                     GEIKAEHGPYAFGTFSSSRSTNELNYLAGKFVREVLGNSNIDSCNRAUHAASVTGLVA
                     TFGAGGATTSYEEFEHTDVILCWGSNTMENHPIIYNHMRRGIKNGAKMIVIDPRKIDQ
                     VRKADKWLGLNVGTDICLANAMGHVIIEEDLYDKPFVERATKGFEAYREKVAGYTPEY
                     AEQITGVPADDIREVARMYANADRATLNWTLGVTEHHNGADAVFALIALGLLTGHVGK
                     YGSGLNPLRGQNNVQGGGDMGALPDKMVGGWLWNDPEARELFEDVWGVPLPEKKGKNQ
                     AEMMEAVKSGEIRCMYVIGENPVQSDADENKHESLFGKLDFLVVQDILMTKTARMADV
                     VLPAAASWAETDGTVINSERRVQRVRKAVDPPGEARDDIMILRDIARRMGYDWNFETA
                     EEVWEEVRLLAPKFSGISYERLQTHGGIQWPCHDGSFEEISDISPKEELMASTRGAEN
                     AGGEAITTDVVEREGPGTKFLHAELRAEEVDDPVPFTPVDHEGPVEMP"
BASE COUNT          683 a         1017 c         1085 g          475 t
ORIGIN      
        1 ggccctgcga ccccggcggc cgaccacgcc gtcagcagcc gcccgtggat ggccccaacc
       61 tgcgccacgc cgaacaggtc cttcaggtag gccgggatgg tcgcgaaccc gccgccgtag
      121 aagctcagga tcacgagggt cagcacgagg aacatggcga gcgacgccga tccgaaagag
      181 gccagcacga agtacatgac cgttccgacg ccgaggtaga ccatgtagat cggcttgcgt
      241 cctatgtagt ccgaggtcga ggaccagccg agcctccccg ccatgttcgc caacgagagc
      301 aggccgacga acccggccgc agccgccgcc gtcacggcga agaagtcctg gatcatgggc
      361 gcggcctgct ccagaagacc tatgcctgct gtcacgttgc agaagagcac tgtccacaac
      421 aaccagaact ggggcgtgcg gatcgcgctg tccgccgata cgttggcggt ggtctcgagg
      481 gccgaggtct cctcctcggt tggcggcgtc cagccttccg gctgccagtc ctcggcgggg
      541 aggcgcacga cgaacgcccc gatcatcatg gcgatcaggt agatcacacc gagcgtcagg
      601 aaggacggca cgatcgcgtc caccgggttg ctggcgtagg ccgtgagcag cctgtccgtg
      661 agcggcgagg cgatgagggc tccaccgccg aagcccatga tcgccagacc cgtggccagc
      721 ccgggtcggt cggggaacca tttgatcagg gtcgagaccg gcgagatgta cccgatgccg
      781 agaccgatgc cacctatgag accgtagccc aggtacacga gccacagctg cgaggtggcg
      841 acgccaaggg cgcttatgaa gaacccgctc gaccagcaga gcgcggccgt gaacatcgcc
      901 gcgcgcggcc cggcacgctc cacccaggtg ccgaagaccg cggcggagag gccaagcatc
      961 acgatgctta tgctgaagat gatcgcgatg ggcgtcaggc ccgtcccgaa ccgctctacc
     1021 agcggggtct tgaacacgct gaaggcgtag atctcgccta tgctcagatg caccagcagg
     1081 gcagccggtg gtatgagcca cctgttgaac ccgggccccg caatcgttct atccctcgcc
     1141 agaaaactga tggcaccctc cctaggaacc cccatacgtc aggccgtacg ttagtttgat
     1201 atcaagctcg tgtcaaccag ggcgcggcgt tggcagcaga accgcacgcc tggagacaac
     1261 gggttacagg tcgcgggctt caggaaaatc gcctgatgca tgacgccttt tcccgcttcc
     1321 cgacaggcgg ctcctggtag gcaacctggt aggcaattct catcctggaa gttagaatca
     1381 ggtgctacag ggaacccggt tcgagaaggg gtgagtcgca tggcgacggc tatagagaaa
     1441 gtctcggaga acggcaaggc cgacggcgca cgggaagtgg ccacgatctg cggtttctgt
     1501 ggtgttggat gtggcctgat cctcaaggtc gaggacggcg tcatccagaa ggtaacgggc
     1561 gagaaagaga acccgtccag caggggcgag gcgtgcatca aggggcgcga gggctggcgt
     1621 cacctgtaca gcgaccggag gatcacgcgc cccctcatcc gcaaggacgg cgagctcgtt
     1681 ccggcctcct gggatgaggc gctcgacctg gtggccagca agatgggtga gatcaaggcc
     1741 gagcacggcc cctacgcctt cggaaccttc agctcctcgc gttcgaccaa cgagctcaac
     1801 tacctggctg gcaagttcgt cagggaggtg ctcgggaaca gcaacataga ctcatgtaac
     1861 cgtgcttgac acgccgccag cgtcaccggt ctggtggcaa cgtttggagc gggcggggcg
     1921 accacctctt acgaggagtt cgagcacacg gatgtaatat tatgctgggg ctccaacacc
     1981 atggagaacc accccatcat ctacaaccac atgcgccgcg gcatcaagaa cggcgcgaag
     2041 atgatcgtta tcgacccgcg taagattgac caggtcagga aggccgacaa gtggctcggc
     2101 ctcaacgtcg gcaccgatat ctgcctggcc aacgcgatgg gtcacgtcat catcgaggaa
     2161 gacctctatg acaaaccctt cgtcgagcgc gccacgaaag gcttcgaggc gtacagggag
     2221 aaggttgccg gctacacgcc agagtacgcg gaacagatca cgggcgtccc ggcggacgac
     2281 atccgcgagg tggcccgcat gtacgccaac gcggacaggg cgacgctgaa ctggacgctt
     2341 ggcgtcaccg agcaccacaa cggcgctgat gcggtcttcg ccctcatcgc actcggcctc
     2401 ctgacgggcc acgtcggcaa gtacgggagc ggcctgaacc cgcttcgcgg ccagaacaac
     2461 gtgcagggcg gcggcgacat gggcgcgctg ccggacaaga tggtcggcgg gtggctctgg
     2521 aacgacccgg aagcgcgcga gctgttcgag gacgtgtggg gcgtaccgct gcccgagaag
     2581 aagggcaaga accaggccga gatgatggag gccgtgaaga gcggcgagat ccggtgtatg
     2641 tacgtgatcg gggagaaccc cgtccagtcg gacgccgacg agaacaagca cgagagcctc
     2701 ttcggcaaac tggactttct cgtcgtccag gacatcctca tgaccaagac cgcccggatg
     2761 gcggacgtgg tactgccggc cgcagcgagc tgggccgaga ccgatggcac ggtcatcaac
     2821 agcgagaggc gcgtgcagcg ggtgcgcaag gcagtcgacc cgcccggcga ggcgcgggac
     2881 gacatcatga tcctgcgcga catcgcgcgc cgcatgggat acgactggaa cttcgagacc
     2941 gccgaggaag tctgggaaga ggtgcgcctt ctcgcgccca agttcagcgg catcagctac
     3001 gagcggcttc agacgcacgg cggcatccag tggccctgcc acgacgggag cttcgaggag
     3061 atctccgaca tctctccgaa ggaggagctc atggcgagca ccaggggtgc cgagaacgcc
     3121 gggggcgagg ctatcaccac ggacgtggtc gagagagagg gtccaggcac caagttcctg
     3181 cacgccgagc tgagggccga ggaggtcgac gaccccgtcc ccttcacgcc cgtggaccac
     3241 gaaggtccgg tcgagatgcc
//
LOCUS       DBNGVD010000103         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1114888, whole genome shotgun sequence.
ACCESSION   DBNGVD010000103 DBNGVD010000000
VERSION     DBNGVD010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1114888"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..437)
                     /locus_tag="AC16CM_02995"
     tRNA            complement(352..437)
                     /locus_tag="AC16CM_02995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Leu,seq:caa)
     gene            596..>1707
                     /locus_tag="AC16CM_03000"
     CDS             596..>1707
                     /locus_tag="AC16CM_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3138824.1"
                     /translation="MRRTRRVTGLVAVGAIMALVAIGCGGGGGDSGGGGGGGGEAPTA
                     KIVSDLPRQGANRAQTTTMVNAINLALDERNNKAGDINIEYESLDDATAQAGQWDAAK
                     CAENAQSAAQDDEIIGWIGPFNSGCAAVEIPILNEAGLAMISPANTYTGLTKPTPDES
                     EPDKYYPTGQRNYARVIVTDVQQGQAGASFMDEEGVESVYILDDRETYGKGVADEFQT
                     AAEDLGIEVLGREGIDGSAPNYRSLMNKVAAADPDAIYFGGIIENNAAQIIKDKVGAG
                     MSNEEVIFVGPDGIFVDELITQGGNSVEGIYVSFGGIPPEKLGSAGQEFIDKYEETYD
                     DDVQPYTAYAYEAANVMLDAIERASEDAGGDVPDRQA"
BASE COUNT          354 a          536 c          560 g          257 t
ORIGIN      
        1 cccgttcagc caccatccaa ctcccttctt agcacgctcc ggctacgcct acgctcatca
       61 gcacactcgg gctttcgccc tcgcttgtca gctcgcatcg cccggcttcg cctagagctc
      121 gctttcagca cgctcccttc ggtcgctttc agccgatcac gctgcggcct gcaccctgcc
      181 actctagagg ttcgatgctc gagaaaggtg ggtagtcgaa gctgctgtta cggagagaga
      241 cgaccagctg aaaacgaggc gaagccgaag cgttgctgac aagcgaaggc gctagcccga
      301 gcgtgctgac aagccgcgaa gcggcgtgcg cgtaggccga aggccgtagc ataccggagg
      361 tcgggctcga accgacacgg tgctctcgca ccaccggatt ttgagtccgg cgcgtctacc
      421 aattccgcca ctccggcgtg agaagccgcg acattatacc aacgtcccgg gctctttcgg
      481 gagattgcgt tgcttgacaa gcgggagcat ccctattgat aatgccggcg gaacggggta
      541 cttgcaggct accatctggc gcatcgctgt cgcgagccgg gcacgaggag gagcattgag
      601 gagaacacgc cgagtaacgg gtctcgtggc ggtgggggcg ataatggccc tagtcgccat
      661 cgggtgcggt ggcggtgggg gcgatagcgg cggtggaggt ggaggcgggg gagaggcacc
      721 cacggccaag atcgtgagcg acctgccgag gcagggggca aaccgggcgc agaccaccac
      781 catggtgaac gcgataaacc tggccctcga cgagcggaac aacaaggccg gcgacatcaa
      841 catcgagtac gagagcctgg acgacgccac ggcccaggcc ggccagtggg acgccgccaa
      901 gtgcgccgag aatgcccagt ccgccgccca ggacgacgag atcattggtt ggatcggacc
      961 attcaactcc ggctgcgccg cggtggaaat tccgatcctg aacgaggcgg ggctcgccat
     1021 gataagcccg gcgaacacct acaccggcct gaccaagccg acccctgacg agtccgagcc
     1081 tgacaagtac tatccgacgg gccagcgcaa ctacgcgcgc gtcatcgtta ccgacgtgca
     1141 gcagggccag gcgggcgctt ccttcatgga cgaggaaggc gtcgagagcg tctacatcct
     1201 cgacgacagg gagacctacg gcaagggcgt ggcagacgag ttccagacgg ccgccgagga
     1261 cctcggtata gaggttctgg ggcgtgaggg catcgacggc tcggccccca actaccgctc
     1321 gctcatgaac aaggtagccg cggctgaccc cgacgccatc tacttcggcg gcatcatcga
     1381 gaacaacgcg gcccagatca taaaggacaa ggtcggcgcc gggatgagca acgaagaggt
     1441 tatcttcgtg gggcccgacg gcatcttcgt ggacgagctg atcacccagg gcggcaactc
     1501 cgtcgagggc atctacgtct cgttcggcgg gatccctccg gaaaagctcg gttccgcggg
     1561 ccaggagttc atagataagt acgaagagac ctacgacgac gacgtgcagc cctacacggc
     1621 ctacgcctac gaggcggcca acgtgatgct cgacgccata gagcgggcat ccgaggacgc
     1681 gggtggcgac gtgccggacc gccaggc
//
LOCUS       DBNGVD010000104         4169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3847433, whole genome shotgun sequence.
ACCESSION   DBNGVD010000104 DBNGVD010000000
VERSION     DBNGVD010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3847433"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16CM_03005"
     CDS             complement(<1..181)
                     /locus_tag="AC16CM_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138825.1"
                     /translation="MNGAANKQAYERYIGLLNAQDFGALDEVVDPERYREICVGFTPG
                     WVNLPEAVASLEKVVG"
     gene            complement(296..472)
                     /locus_tag="AC16CM_03010"
     CDS             complement(296..472)
                     /locus_tag="AC16CM_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138826.1"
                     /translation="MSPRDAALALLCTILALARVSKNRKISHGNMVLIELCLGLVSVG
                     ASVFWLKVLALALG"
     gene            complement(469..963)
                     /locus_tag="AC16CM_03015"
     CDS             complement(469..963)
                     /locus_tag="AC16CM_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138827.1"
                     /translation="MLLMDLNEQADKDFGAARRRAWMRRLGSSLRGKPAGGPQASFRD
                     VQNRRRAHNRVRRGVRTVDVDRIVGSVGRSKDFDSGFLPLRASTGARWKRVDVAFHRG
                     ADLPPVSLYEIEGDFFVLDGNHRVSVARFHGLRTVEAEVTELLPPHSGSPTPEAVVLE
                     ARAA"
     gene            complement(1087..3810)
                     /locus_tag="AC16CM_03020"
     CDS             complement(1087..3810)
                     /locus_tag="AC16CM_03020"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3138828.1"
                     /translation="MAEGMLADRQMLRAKLYVPRGRPGAVARARLYERLDEGVRRDLT
                     VISAPAGFGKTTLLADWSRQSELPVAWVSLDERDDDPIRFLSYLLAAIGTIHEGFGEP
                     TRAFLRSLPSGDELEPVLTALSNEILDLPSDFVLVLDDYHVLRSREIHEALAFLLEYA
                     PPPMRLVLAGRGSPPLPLASLRAEGRLTELGEADLRFTVDEAADFLGGTMGLDLSGEG
                     VAALEDGTEGWIVGLLLAAHAVKGRGDSADGPESLPGGTRHVFDYLAEEVLSRQPEEV
                     REFMLSTSVVETLSAPLCEALTDTEDARSMLERLERDNLFLVPLDEEGRYYRYHHLFA
                     AFLREHLGRTHPGAVAGLHRRAGLWYERDRCLTGAVEHALAAGDFGWAADLIEEETGA
                     RRRYVDASQLRLWLDALPERMVSTRPKLCLLGAWALAHSGELEDAERRLLHTEAALGL
                     DIDVPTIDDLSDEVRTMLGEVCIIRARLAALREQAPLTTRLSNRALQLLPEHEVRLRG
                     GVALDLGHAYCSTGEFDLASEAFAEAAATGRGADNLRTALFAFHYQAQLEIARGHLGK
                     AESLLLDGKRLAEDHPDSVPSVAGIIDVGLGDLAYQRGDLDAARRLFEAGIERGTQSG
                     EAKILVYGYVDLARVCVARGDADTALSLVRRAGRLTPRWPLIWAWQARFWLAQGDVAS
                     AARWAREYGTSEDYLNYPRHLERISMTRVLLAEGRTEEGFEALEGLLAEVRSSGMTAY
                     EMELLVLLALASERSGETEQAMDLLKAALALAEPEGFVRVFLDEGPPMAALLRTLIQH
                     HHNGGLRTSPNGSPEGYAGTLLERFALEAQPAGNKTTREAPAPGIEPLSDRETEVLSL
                     VADGRTNSQIAAELYLSVGTVKAHVHHIFGKLLVRNRSQAVARARELRLLD"
BASE COUNT          708 a         1395 c         1348 g          718 t
ORIGIN      
        1 cgccaacgac cttctccagc gaggcaaccg cctcggggag gttcacccag ccgggcgtga
       61 atccaacgca gatctcacgg taacgttcgg gatccacgac ctcgtccagt gctccgaaat
      121 cctgcgcgtt caagagccct atgtagcgct cgtaggcttg cttgttggcc gcaccgttca
      181 cctttctcct ctcagggtac gccaggaaca gaaccaatca tcccagatgc gatggttcgt
      241 gtcgcctacc cgtaaggtca ggtcgggggc cttctggggc gtgaccagac ggtcttcaac
      301 ccagggcgag ggcgagaacc ttgagccaga acacggaggc gccgaccgac accaggccaa
      361 ggcaaagctc tatcaagacc atgttaccgt gcgatatctt ccggttcttc gagacacgcg
      421 cgagggccag gatggtacac agaagcgcga gggcggcgtc ccgcgggctc atgcggccct
      481 cgcttccagt actacggcct cgggggtcgg gcttccggag tgtgggggca ataactccgt
      541 gacctccgct tccacggttc gcaacccgtg gaaacgggct accgagacgc ggtggttgcc
      601 atccagaacg aagaagtcgc cctctatctc gtaaaggctc acgggtggaa ggtccgcgcc
      661 cctgtgaaag gctacgtcga cgcgcttcca ccgcgcgccc gtgctcgcac gcaggggcag
      721 gaaaccgctg tcgaagtcct tgctgcgacc cacgctcccc acgatcctat cgacgtccac
      781 ggtgcggact ccccttcgca ccctgttgtg cgccctccgc cggttctgaa cgtcccgaaa
      841 agaagcctgc ggaccgccgg caggcttccc acgaaggctc gagccaagcc tccgcatcca
      901 ggccctgcga cgggcggccc caaaatcctt gtctgcctgc tcgttgagat ccatcagcaa
      961 catctctgaa ctcccctcta ctgaacctgt gatgaggtta agggccagcc aggcatgctc
     1021 catctagcga aggtcgtatc tggtggttta tgggaggttt aattggagcg gtcagctgtc
     1081 agctatctaa tccagcaggc gcagttcgcg ggcccttgcg acggcctggg agcggttgcg
     1141 caccaggagc ttgccgaaga tgtggtgtac gtgcgctttg acggtgccca cggagaggta
     1201 gagttcggcg gcgatctgtg agttggtgcg gccgtcggcg accagcgaga ggacttccgt
     1261 ctcgcggtcg ctgaggggct cgatgccggg ggctggcgcc tccctggtgg ttttgttgcc
     1321 cgcgggttgc gcttcgaggg cgaagcgctc gagcagggtg cctgcgtagc cttctggaga
     1381 gccgttcggg gatgtccgga gcccaccgtt gtggtggtgc tggatcaggg tcctcagcag
     1441 ggcggccatc ggcggtccct cgtcgaggaa cacgcgcacg aagccctccg gctccgcgag
     1501 cgccagcgcc gccttcagga ggtccatcgc ctgttcggtc tcgccgctcc gttccgaggc
     1561 gagcgccagc aacacgagca actccatctc gtaggccgtc atccccgagg accggacctc
     1621 agcaagcagt ccctccaggg cctcgaaacc ctcttcggtt ctgccctctg cgaggagcac
     1681 gcgggtcatg ctgatgcgct ccaggtgacg cgggtagttc aggtagtctt cagaggtccc
     1741 gtactccctg gcccaacgcg ccgcggacgc cacgtcgccc tgggccagcc agaaccgcgc
     1801 ctgccaggcc cagatgagcg gccatctcgg cgtgagcctg ccggcccgcc gtacgagcga
     1861 gagcgcggtg tctgcgtcgc cacgggcgac gcagacacgg gccaggtcga cgtacccgta
     1921 gaccaggatc ttcgcctccc cgctctgcgt gccgcgctcg atccctgctt cgaagagacg
     1981 acgcgccgcg tccagatccc cgcgctggta ggcgaggtcg cccagcccga cgtcgatgat
     2041 gccggcgacc gagggcacac tatcggggtg atcttcggcc agccgcttcc cgtcgagcag
     2101 caggctctcc gccttcccga ggtgtccgcg cgcgatctcc agctgggcct ggtaatggaa
     2161 ggcgaagagg gccgtcctga ggttgtctgc gccccggccc gtggccgctg cctcggcgaa
     2221 cgcctcgctt gccagatcga actcccccgt cgagcaataa gcgtgtccca ggtccagcgc
     2281 cacgccgccg cgcaaacgta cttcgtgctc cggcaacagc tggagggccc tgttggagag
     2341 cctggtggtt agcggcgcct gctcccgcaa ggcggccagg cgggcgcgga tgatgcaaac
     2401 ctctcccagc atggtccgta cttcatcgga gaggtcgtcg atggtgggga cgtcgatgtc
     2461 gaggcccagg gcggcctccg tatgcaggag gcggcgctcg gcgtcttcca gctctccgga
     2521 gtgggcaagc gcccaggcgc cgagcagaca gagcttcggc cgggtactca ccatcctttc
     2581 gggcagggcg tccagccata gtcgcaactg ggaggcgtcg acgtacctgc gtcgcgcgcc
     2641 ggtctcttcc tcgatcagat ctgctgccca cccgaagtcg cctgcggcca gggcgtgctc
     2701 cacggccccg gtcagacagc ggtctcgctc gtaccagagc ccggctcgac ggtgcagacc
     2761 ggctacggcg ccaggatgcg tgcgccccag atgctcgcga agaaaggcgg cgaagaggtg
     2821 atggtagcgg tagtagcgtc cttcctcgtc cagcggaacg agaaacaggt tgtcccgctc
     2881 cagcctctcc agcatcgacc ttgcgtcttc cgtatcggtg agcgcctcgc agagcggggc
     2941 gctcaaggtc tccacgacgg aggtagagag catgaactct ctgacctctt ccggttggcg
     3001 ggagagcact tcttcggcca ggtagtcgaa gacgtgacgc gtaccccccg ggagagactc
     3061 cggaccgtcc gcgctgtcac cgcgtccctt cacggcgtgc gccgccagca ggaggcccac
     3121 gatccaaccc tccgttccgt cctcgagggc cgcgacgccc tcgccggaga gatccagccc
     3181 catggtgccg ccgagaaagt ccgcggcctc atcgacggtg aagcgcaggt cggcctcgcc
     3241 aagctcggtc agccttccct cggcgcgcag gctcgcgagc ggtagcggcg ggctcccacg
     3301 cccggcgagg acgaggcgca tcggcggcgg cgcgtactcc agcaagaaag ccagagcctc
     3361 gtggatctcc cgggagcgca gcacgtggta gtcgtccagg acgagcacga aatcgctggg
     3421 caggtccagg atctcattgc tcaaggcggt gaggacgggc tccagttcgt ctcccgaggg
     3481 cagggagcgg aggaaggctc gcgtgggttc cccgaatcct tcgtggatcg tgccgatagc
     3541 cgccagaagg taggagagaa agcggatcgg gtcgtcgtcc ctctcgtcca gcgagaccca
     3601 ggccacggga agctcgctct gcctgctcca gtccgcgagc aacgtggtct tgccgaatcc
     3661 ggcaggcgcg ctgatgaccg tgagatcgcg ccgcaccccc tcatccagcc gctcgtacag
     3721 acgcgcacgg gcgacggcgc ctggcctccc acgtggcacg tagagcttgg ccctgagcat
     3781 ctgacgatcc gcgagcatcc cctctgccat ccccgaaatt gtactcgatg gcacccatga
     3841 ttccgacgaa cgaatcagcc gaaaagagcg gccgggctcg taataggagg gggatgatac
     3901 cggcccggcc atttgtatgt acgaggcgag aggccgttgg tttcattcgg cgtgggatcc
     3961 ggtcatacgt ctacggctct gcgcgaacgc caacgctctc ggcaggccgt ggattcccgc
     4021 ttccgcggga atgacggaca agaagatccg tgcatgttcg tgcccgggga tgagggggtt
     4081 ctggaagccc agacgctcac ctatcctgga cgcaccgtga ttcctcatcc gattccttgc
     4141 ttctgccaca ttcgctcaac tgactcatt
//
LOCUS       DBNGVD010000105         4938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2208069, whole genome shotgun sequence.
ACCESSION   DBNGVD010000105 DBNGVD010000000
VERSION     DBNGVD010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2208069"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="AC16CM_03025"
     CDS             complement(<1..446)
                     /locus_tag="AC16CM_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3138829.1"
                     /translation="MGWRNGGYASVLLVAVGAAMWGTDAVLRVPLLEVASPSQIVLLE
                     HLVLLLYSIPAVALGWRYFRGLGAAQWVALLVIGWGGSAVATLLFTQAFVIGNPTVVI
                     LLQKTQPLFAIALAGLLLRERLGWAYWPCFAVAMVGAYMISFSDLGP"
     gene            complement(450..1454)
                     /locus_tag="AC16CM_03030"
     CDS             complement(450..1454)
                     /locus_tag="AC16CM_03030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3138830.1"
                     /translation="MKHTGTVGYAAMFEQFHPTELLGWCKVAEESGFGSVMASDHFHP
                     WTPDQGQSAFVWSWMGALGATTNLRFGTGVTPPGFRYHPAIIAQGAATLEAMYPGRFW
                     LGLGAGEALNEHVVGDYWPEAPTRLRILMEAIEVIRRLFSGKVVKYSGDHIKLESAKL
                     YTMPDTPPPIYVATAGPLQSRRTGEFCDGIITVGAADEKIKMLMEKFEEGARRGDKDP
                     SEMPRMIQLHVSWADSQQEAEEQSVREWPNGGMPFPKADIRNPEDFAAMAKQVSVDNF
                     VNRVLMSADLDEHLEHIQHYVDLGFDEVYVHNVGRNQEEFIKAYGERVIPKLRWPGNG
                     "
     gene            complement(1637..2821)
                     /locus_tag="AC16CM_03035"
     CDS             complement(1637..2821)
                     /locus_tag="AC16CM_03035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138831.1"
                     /translation="MMELRRYREGDAETLARLGVAAFDTSVSDWMEMFDPGRNARLDL
                     EQVHVIEEDGEPRASATVLPLESFVGGVPRPMGGISAVMVHPAYRRRGYAGKLMRAAL
                     RDMREREVALSLLSPFAHAFYRVFGYELASETIQYSLKPSDLSTSPEQGLLRDYREGD
                     LPSLMEAYEAEARRHALSVRRSRDHWEEILAEKDGYVAVYEREGGVEGYILYKLSGWQ
                     EQDPRRTLKVNELVALGPRANEALLSFLAGFDPLVYVHGIKLTTSRGEPLHPYLQSSY
                     VKASIDPDQMLRLVDVETALGYLELASDEPLVLDVRDDVIAENQGEYTIGQGKVVRGA
                     EAEQSITLDVRQLTQLYAGYLPAADLARRDLIQPSSPEALELLDSLFPLGDPLLSRPD
                     HF"
     gene            complement(2989..3582)
                     /locus_tag="AC16CM_03040"
     CDS             complement(2989..3582)
                     /locus_tag="AC16CM_03040"
                     /inference="COORDINATES: protein motif:HMM:NF021886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3138832.1"
                     /translation="MRTLGYAVLGLLSREELSGYDLKRWMERPLGYFWSARHSQIYPE
                     LAQLEEEGLVAHTLVEQSGKPDKKVYRITAEGLRALEDWVTQPPGSRPVRDELTLKAY
                     SVWLTDKEKAARLFREEGLQHEGRLAHYEDLRAWMEGEHQDEMRDPASPWFATYATLR
                     RGIGYEKEYAEWCRWMADSMQEREPVEAPKNSEASGK"
     gene            3698..4222
                     /locus_tag="AC16CM_03045"
     CDS             3698..4222
                     /locus_tag="AC16CM_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138833.1"
                     /translation="MRKAGLMCGRSYAGVGFGSGVVRWWGHHGRGEFFGLDVEKMSPG
                     TRAVAALSVILPVALSGLLLVVFVPQLWWVFTTYFWIAFPAIGLLSGSVSEIGERTPR
                     ISEAELERELLEAIRDGGEVSAAAAAARTSLSVAEADRRLRALAENGHIAVRARGGGV
                     FYSLWGIGDGGSAP"
     gene            4219..4653
                     /locus_tag="AC16CM_03050"
     CDS             4219..4653
                     /locus_tag="AC16CM_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138834.1"
                     /translation="MSRESRLTAGVLLVLLPAVVYGGTSILSLLIGDPAYAQNGLRQD
                     LWRAGHAHAGVLLVLSLVTLRYVDEANLPGWLAGFARHSVPAAAVLLPAAFFLSVLSP
                     EATEPNAMIYLAFVGAVVLASGVFVLGVGLIRKPRIRKTEVR"
     gene            4735..>4938
                     /locus_tag="AC16CM_03055"
     CDS             4735..>4938
                     /locus_tag="AC16CM_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase family protein"
                     /protein_id="HIR3138835.1"
                     /translation="MAQIVNGRHMARIDEPFVVFIIGMGINNVFAVRKWLPTLRAMGP
                     MLRELYKHPEKGFLGGEFFFYGRG"
BASE COUNT          807 a         1585 c         1573 g          973 t
ORIGIN      
        1 ggcccgaggt cggagaagga gatcatgtaa gcccccacca tcgccaccgc gaagcagggc
       61 cagtaggccc acccaagccg ctcgcgcagc aggaggccgg ccagcgcgat ggcgaacagc
      121 ggctgggtct tctggagcag gatcaccacg gtcgggttcc cgatcacgaa cgcctgcgtg
      181 aagagcaggg tcgcgactgc cgaaccgccc cacccgatga ccagaagggc tacccattgg
      241 gcggcgccaa ggccccggaa gtacctccac ccgagcgcca ccgcggggat cgagtacagt
      301 agcaggacca ggtgctcgag cagcacgatc tgggagggtg atgccacctc cagcaagggc
      361 acccgcagca cggcgtccgt gccccacatc gcagccccca cggcgacgag caggacgctt
      421 gcgtacccgc cgtttctcca gcccacgcgc taaccgttcc cgggccagcg gagcttcggg
      481 atgacgcgct cgccgtaggc cttgatgaac tcttcctggt tgcgtcccac gttgtggacg
      541 tagacctcgt cgaagccgag atcgacgtag tgctgtatgt gctcgaggtg ctcgtccagg
      601 tctgcggaca tcaggacccg gttcacgaag ttgtcgacgc tcacctgctt tgccatggcc
      661 gcgaaatcct cggggttgcg gatgtcggcc tttgggaagg gcatcccgcc gttgggccac
      721 tccctgacgg actgctcttc ggcctcttgc tggctatctg cccacgagac gtgaagctgt
      781 atcatgcgcg gcatctccga ggggtccttg tccccgcggc gcgccccctc ctcgaacttc
      841 tccatcagca tctttatctt ctcgtctgct gcccccacgg ttatgatgcc gtcgcagaac
      901 tcacccgtcc tgcggctctg gagggggccg gcggtggcga cgtagatggg gggcggcgtg
      961 tccggcatcg tgtagagctt cgcgctctcc agctttatat ggtcgccgct gtacttgacg
     1021 accttccccg agaagagcct gcggatgacc tcgatggcct ccatgagtat gcgcaaacgg
     1081 gtgggcgcct ccggccagta atcccccacg acgtgctcgt tcagggcctc gcccgcgcca
     1141 agcccgagcc agaagcgccc ggggtacatg gcctctagcg tcgccgcccc ctgcgcgatg
     1201 atggcgggat ggtaccggaa gccaggcggc gtcacgccag taccgaagcg gaggttcgtc
     1261 gtcgcgccga gggcgcccat ccacgaccag acgaaggcgc tctggccctg atccggggtc
     1321 cacgggtgga agtgatccga ggccatgacc gatccgaaac ccgactcctc tgccaccttg
     1381 caccagccca atagctccgt aggatggaac tgctcgaaca tcgccgcata tcctacggtg
     1441 cccgtgtgct tcatgtcgct cccttcggtc gctagctatc agctgtcagc cgtcagctct
     1501 tgctcggatg gagtagccct ttattatttg ggacacagtt tacagttgcg gcacgcaagg
     1561 tgattttcgg tggcgagaat ggttttgcca ccgggcctga aacctgtagc cagatatctc
     1621 cctcaagata cctccctcaa aaatgatctg gcctgctgag cagcgggtcc ccgaggggga
     1681 agagcgagtc gaggagttcg agggcctcag gggagctcgg ctggatgagg tcgcgcctgg
     1741 cgaggtctgc tgcagggagg tagccagcgt agagctgggt gagctggcgc acgtcgagcg
     1801 tgatgctctg ttcggcttcg gccccgcgga ccactttgcc ttgcccgatg gtgtactcgc
     1861 cctgattctc ggctatgacg tcgtctctca cgtcgaggac gagcggttcg tctgacgcga
     1921 gctctagata gcccagggcg gtttcgacgt cgaccaggcg cagcatctgg tcggggtcga
     1981 tgctggcctt tacgtaggag ctctggagat aggggtggag cggctctccg cgcgaggtgg
     2041 ttagctttat cccatggacg tagaccaggg gatcgaagcc agccaggaaa gagaggagcg
     2101 cctcgttggc ccgcggcccg agggcgacga gctcgttcac cttgagggtg cgtcgtgggt
     2161 cctgttcctg ccacccggag agcttgtaga ggatgtaacc ctcaaccccg ccctcccgct
     2221 cgtagacggc aacgtacccg tctttctcgg caaggatctc ttcccagtga tcccggctcc
     2281 ggcgcacgct gagcgcatgc cgcctcgcct cggcttcgta ggcctccatc agggagggga
     2341 ggtctccctc cctgtagtcc ctgagaagtc cctgctctgg gctggtcgag agatcggagg
     2401 gctttagact gtactggatg gtctcgctgg ccagctcgta accgaagacc ctgtagaagg
     2461 cgtgcgcgaa cggcgagagc aacgacaggg cgacctccct ctcgcgcatg tcgcgcaatg
     2521 cggcgcgcat gagcttcccg gcgtagcccc gccgcctgta ggcgggatgg accatgaccg
     2581 cggagatgcc gcccatggga cgcggtactc cccccacgaa cgactccagc ggcagcacgg
     2641 tcgcgctcgc cctgggctcc ccgtcctctt ctatgacgtg tacctgctcg aggtcgagcc
     2701 tggcattacg cccggggtcg aacatctcca tccagtcaga gacgctggtg tcgaaggcgg
     2761 ccaccccgag gcgcgccagc gtctccgcat ccccctccct gtacctgcgt agctccatca
     2821 tcctccgact ttagccgtca gctttcagct attggccttc agctccttgt atctggctga
     2881 aggccagcag ggcggttggc cctgcgaccc ttccaggcgt ccagattcta ccggaattgc
     2941 gccgttggcg gtcagccgtc agcccactcg ggctgatcgc cgacggcctt actttccgct
     3001 ggcctcactg tttttcggcg cctcgactgg ttcccgttcc tgcatggagt ccgccatcca
     3061 cctgcaccac tcggcgtatt ctttctcgta gccgatgccg cgccggagcg tggcgtaggt
     3121 ggcgaaccag ggagaggccg ggtcccgcat ctcgtcctgg tgctcgcctt ccatccaggc
     3181 tcgaaggtcc tcgtagtgag cgagccgccc ctcgtgctgc aaaccctctt cgcgaaagag
     3241 cctcgcggcc ttctccttgt ccgtcagcca caccgagtac gccttcaacg tcagctcgtc
     3301 ccgcaccggc ctcgacccgg gaggttgtgt cacccaatcc tcgagcgccc gcaacccctc
     3361 agccgtgatc ctgtacacct tcttgtccgg cttgccgctc tgctctacca gcgtgtgcgc
     3421 caccaacccc tcctcctcca gctgcgcgag ctcggggtag atctgactgt gcctcgcact
     3481 ccagaaatat ccaagcggcc gctccatcca ccgcttcaag tcataccccg atagctcctc
     3541 cctggataac aaccccaaca ccgcataccc caacgtcctc atgatggtct ccagaactct
     3601 ccgttgtttt gctatgtcac ccttgacata ttaaccggtc acgcctagga tatctatgtc
     3661 aatgttgaca tatagacgta cagggggtgt ggaaagcgtg aggaaggcag gactgatgtg
     3721 cggtagatct tatgccgggg ttgggttcgg atctggagta gttcgctggt gggggcacca
     3781 cgggcgggga gagttcttcg ggctggatgt ggagaagatg agcccgggca ccagggccgt
     3841 cgccgcgctg tcggtgatcc tgccggtcgc gctctctggg ttgttgctgg tcgttttcgt
     3901 gccgcagttg tggtgggtat tcacgaccta cttctggatc gctttccccg cgatcgggtt
     3961 gctctccggt agcgtttcgg agatcgggga gaggaccccg cggatctccg aagctgagct
     4021 ggagcgggag ttgctggagg cgatacggga tgggggcgag gtatcggccg cggctgcggc
     4081 ggcgaggact tctttgtcgg tggcggaggc ggacagaagg ctaagagcgc tggccgagaa
     4141 cggccacatc gcggtacggg cgcgcggcgg cggcgtcttc tattcgctgt gggggatagg
     4201 cgatggcggg tcggcgccat gagccgcgag tctcggctga cggcgggcgt gctcctggtg
     4261 ttgttacccg cggtcgtcta cggagggacg agcatcctct cgctcctgat cggcgacccc
     4321 gcctacgcgc agaacgggtt gcgccaggac ctctggcggg ccggacacgc gcacgccggg
     4381 gtgctgctcg tgctctcgct ggtaacgctg cgctacgtgg acgaggcaaa cctgccgggc
     4441 tggctcgcgg gctttgcccg gcactccgtc ccggcggcgg cggtgctgtt gcctgcggcg
     4501 ttctttctct ccgtgctctc accggaagct acggagccga acgccatgat ctacctcgcc
     4561 ttcgtgggcg ccgtcgtgtt ggcctcgggt gtcttcgtgc tgggcgtggg ccttatcagg
     4621 aaaccgagaa tccggaagac ggaggtccga tgaagatccg cagaatagta tgtttcgtgg
     4681 tccctgccag ggatcagctg tagttccccg agcctagaga aagggaaggc tggcatggca
     4741 cagatagtaa acggcaggca catggctcgc atagacgagc ccttcgtcgt gttcattatt
     4801 gggatgggaa tcaataacgt cttcgccgtg aggaaatggc ttcccaccct acgcgcgatg
     4861 gggcccatgc tccgcgagct ctacaagcac ccggagaaag ggtttctcgg cggcgagttc
     4921 ttcttctacg ggcgggga
//
LOCUS       DBNGVD010000106         6129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_750849, whole genome shotgun sequence.
ACCESSION   DBNGVD010000106 DBNGVD010000000
VERSION     DBNGVD010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_750849"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1227
                     /locus_tag="AC16CM_03060"
     CDS             109..1227
                     /locus_tag="AC16CM_03060"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR3138836.1"
                     /translation="MAQAQELRQTITSVADLGGPILSAYLSVNARVPENQGRTYLVRL
                     RDAMNDAGVPKELQQRIRDYLDEETHPAARTLAIFANEDDLLEVYRLQVDVPESARWG
                     DPNVGPLMLVLDEYEPYGAAVLDAERFRYFVVSPLSSAHGEEEIKANGFREIDLHPSE
                     PYPRGGGSTDLDPAGRKQDANIHDFYKALGERTRDISFQEGVKRLILAGPRERTAEFR
                     RTLPGELQERVVAEEHVDISESEGKLLDDLETIREGAEHGRQRELLDEIRERGVRGPD
                     ETITALQEENRVYHLAALWEMEAEIRWCDNDGLAIQDVTAEECPYCGQETRVRPLTDV
                     LIELSTARGARLDFMLGENENTDVLRDEFGGMAGLTRF"
     gene            1285..1959
                     /locus_tag="AC16CM_03065"
     CDS             1285..1959
                     /locus_tag="AC16CM_03065"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3138837.1"
                     /translation="MADYEAAWDSFVGERRLEFGGHNDPSWQEGHRYSASLVVPGDAS
                     RFHERLEPLREALRPFSFVSLHPDHFMHITLILLGFLVDEPRDADEISRGRLREIEGR
                     AREVLSDFPAFTVNLANLNAFPGAAFIEAHDGGKIDQLRDALSVGCGLEKPSGPPHLT
                     LAYFQAPDGTEASEELVCAMAAFRHWPIGEITVDDVEITLLDLDPECPEPEMLARIPL
                     GEAISR"
     gene            complement(1967..2476)
                     /gene="sixA"
                     /locus_tag="AC16CM_03070"
     CDS             complement(1967..2476)
                     /gene="sixA"
                     /locus_tag="AC16CM_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR3138838.1"
                     /translation="MYLYLVRHAVAHKRDPELWPDDSQRPLTPEGEEDFVGAARGLAH
                     IVPEVDVLLSSPYTRAWRTAEILTQHAGWPGPQELPALEPDVPPEKAVLALETYADRQ
                     SIALVGHRPCLHELAVYLLTGDARGADMKIKKGGIVCIEFDEPPGPGTGRLRWLFTPR
                     VLRLSDFKD"
     gene            complement(2666..4741)
                     /gene="ppk1"
                     /locus_tag="AC16CM_03075"
     CDS             complement(2666..4741)
                     /gene="ppk1"
                     /locus_tag="AC16CM_03075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3138839.1"
                     /translation="MTDVARRTSLSDPSLYINRELSWLGFNNRVLEQARDERHPLLER
                     ARFISISETNLDEFFMIRVAGLQQQVASELPNPVPDGMTPEEQLLRIHDHTEEFFEER
                     RRILNTELMPALEAEGIIMVPYHEVSATERRELRERFVREILPILTPLAIDPAHPFPH
                     ISNLSLNVLVVIGNEGRRVMARIKVPTSIDRFVRLPDGPRGQGRRPEMRFVRVEEVIS
                     ANLDELFPGKEVVASYIFQVTRNADFVIEEDEAADLLQAIEDELESRWFGQSVRLVVS
                     DEMPDDLRDWLARKLKLDENSVFESPHPIGLADLEELTHLDRSDLLYPPITPRVPPEI
                     RSARSITQAIRNGDLLLYHPYDSFNPVVDLVRAAANDPEVLAIKQTLYRVGSNSPIVE
                     ALSEARDEQTQVAVLVELKARFDEEPNIIWARQLEARGVHVAYGIVGLKTHAKISLVV
                     RREGAGLRRYVHLGTGNYNPSTARVYTDFSYFTDDPGLAEDGSDLFNYLTGYSEQEEY
                     QELLVAPITLRARIEHLIEEQTEKARNGEEATITCKTNSLTDPHIIELLYEASQAGVK
                     IDLIVRSICCLRPGVEGVSENIRVVSLVGRFLEHARIFAFSDGDDERIYLGSADLMQR
                     NLDRRVETAFPLRERHHRKRVRRLLDLQLADNANGWELGPDGTFERRHPDEGEDQVDS
                     QRILLEEGF"
     gene            complement(4738..5616)
                     /locus_tag="AC16CM_03080"
     CDS             complement(4738..5616)
                     /locus_tag="AC16CM_03080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HIR3138840.1"
                     /translation="MSRHSVIDVGSNTIHLLVGEVDDGAVIPVAGEKVSARLGAGVEK
                     TGRLDERRLDLAAEAIKLFVGIAAMNGTSSPEVLATSAVRDAGNGQELVDRVREATGL
                     EMRLISGEKEAALGFRGALSAVRHEGKVLVVDLGGGSAQLILGDARGGPEERVSLPLG
                     TNRTTERFVSEGPPTDGELGALGEHVGRLLPAWDIGDAEVVAVGGSARAILKITRDGL
                     TADRLGRLSLETRGRASGVLAREHGLAPERARVLPAAITTLAAVLEHFGKGELTVARG
                     GIREGTLLALDEGEVI"
     gene            5866..>6129
                     /locus_tag="AC16CM_03085"
     CDS             5866..>6129
                     /locus_tag="AC16CM_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR3138841.1"
                     /translation="MLVCKGGDCKKRGSKTVRGALKDELRAQHMNRDVRVDSVDCLGL
                     CKHGPNLIVYPGGTWYLGATEHDIPEIVREHLVNGEPVEHLAAE"
BASE COUNT         1079 a         2016 c         1976 g         1058 t
ORIGIN      
        1 cgctccggat gcgggttcgc gggtgcgaat tcgtgtttac gtggccggta ggtaggggac
       61 ctagataggg ccgttctata ccacacacta acgagaggtg tttcaggcat ggcccaggca
      121 caggaactaa gacagacgat aacctccgtg gcggaccttg ggggtccgat cctctcggcg
      181 tatctgagcg tcaacgcaag agtaccggag aaccagggcc ggacgtacct cgtacgcctg
      241 cgcgacgcca tgaacgatgc gggcgtgccc aaagaactgc agcaaaggat cagggactac
      301 ctggacgagg agacgcatcc cgcggccagg acgctcgcca tcttcgcaaa cgaggacgac
      361 ctgctcgagg tctaccgtct gcaggtcgac gttcccgagt cggcccgttg gggggatccc
      421 aacgtcgggc cgctgatgct cgtgctggac gagtacgagc cttacggggc cgcggtgctc
      481 gacgccgaga ggttcaggta cttcgtggtc tcccccctca gcagcgccca cggggaagag
      541 gagatcaagg ccaacggctt cagggagatc gacctccatc ccagcgagcc ctatcccagg
      601 ggtggtggat cgaccgacct cgacccggcc ggccgcaagc aagatgccaa catccacgac
      661 ttctacaagg ctctcgggga gcgcacccgc gacatctcct tccaggaggg ggtgaagcgc
      721 ctcatcctcg ccggccccag ggagaggacg gcagagttcc gtcgtaccct ccccggcgag
      781 ctccaggagc gggtagtcgc cgaggagcac gtggacattt cggagtccga gggcaagctg
      841 ctcgacgacc tggagaccat cagggagggc gcagaacacg gtcgccagag ggagttgctc
      901 gacgagatca gggagcgcgg tgtcaggggg cccgatgaga ccataaccgc gctgcaagag
      961 gagaaccgtg tctaccatct ggccgccctc tgggagatgg aggccgagat ccgctggtgc
     1021 gacaacgacg ggctagccat acaggacgtc accgccgaag agtgcccgta ctgtggccag
     1081 gagacccgcg tgcgcccgct cacggacgtc ctgatcgaac tctccaccgc caggggagcc
     1141 cgcctggact tcatgcttgg cgagaacgag aacacggatg tcctgcgcga cgagttcggc
     1201 ggcatggcag ggctgacgcg attctagggt cgttcccgga acccggcggc gggcggggtg
     1261 ggccctctct gctagagttc ctggatggca gactatgagg ccgcgtggga cagcttcgtc
     1321 ggcgagcggc gcctggagtt cggcggacac aacgacccct cctggcagga ggggcacagg
     1381 tactcggcct cgctcgtcgt tcccggggac gcttcacggt ttcacgagcg cctcgagccc
     1441 ctgagggaag cgctacgtcc tttctccttc gtctcgctac atcccgacca cttcatgcac
     1501 ataaccctca ttcttctggg ctttctggtg gatgagccca gagacgccga cgaaatctca
     1561 cgcgggcggc tgcgggagat agagggccgc gccagggagg tgctctccga cttccccgcg
     1621 ttcaccgtca acctcgccaa cctcaacgcc tttcccggcg cggctttcat cgaggcccac
     1681 gacgggggga agatcgatca gttgagggac gccctttccg tgggctgcgg tctcgagaaa
     1741 ccatcgggac cgccacacct caccctcgcc tacttccagg cccccgacgg tacggaggca
     1801 tcggaagagc tcgtctgcgc catggcggca ttcaggcact ggcccatagg cgagatcacg
     1861 gtagacgacg ttgagattac gctcctcgac ctcgatccgg aatgcccgga gccggagatg
     1921 ctggcaagga taccgctcgg agaagcgatc agccggtagc tgacagctag tccttgaagt
     1981 ccgagagcct gagtacccgc ggtgtgaaca gccagcgtag cctgccggtt ccggggccgg
     2041 ggggctcgtc gaactcgatg cacacgatgc cgcccttctt gatcttcatg tcagcgcccc
     2101 tggcatcgcc ggttagcagg taaacggcca gctcgtgcag gcagggcctg tggccgacga
     2161 gggctatgga ctgcctgtcg gcgtaggttt ctagcgcgag cacggccttc tcgggaggga
     2221 cgtccggttc cagggccgga agctcttgtg gccctggcca gccggcgtgc tgggtgagga
     2281 tctccgccgt gcgccacgcc cttgtgtagg ggctgctcag cagaacgtcc acctcgggca
     2341 cgatatgggc gagaccacgg gccgccccca cgaagtcctc ctcgccctca ggcgtgagcg
     2401 gccgctggct gtcgtcgggc cagagttccg ggtcgcgttt gtgggcaacg gcgtggcgca
     2461 cgaggtagag gtacacggcg gcctaccgga ggcccgcgtc gatcgggcag agacccccgt
     2521 cgagatgacc gtctattccc gaatacttgc cgacttgcct ggtgatcatg cgcctcccgt
     2581 tacgctgtaa caggggcgca atttagcacg ggcacgtacc cggcggcaac gggcaaaaca
     2641 caaacaggcc gtcctgaaca gttacctaga aaccttcctc cagcagtatt ctctggctat
     2701 ccacctggtc ctcgccctcg tccgggtgcc tgcgctcgaa ggtgccgtcc gggccgagct
     2761 cccagccgtt ggcgttgtcg gcgagctgga ggtctagcag tcgtcttacc cgctttcggt
     2821 ggtgtcgctc gcgcaggggg aaggccgtct ccacgcgccg gtccaggttg cgctgcatca
     2881 ggtcagcgct accgaggtag atccgctcgt cgtccccgtc ggagaaggcg aagatgcgcg
     2941 cgtgctccag gaaacgcccc accagcgaga ccacgcggat gttctcgctc acgccctcta
     3001 cgccgggcct caggcagcag atgctgcgca cgatcaggtc tatcttcacc ccggcctggg
     3061 aggcctcgta gagcagctcg atgatgtggg ggtccgtgag cgagttggtc ttgcacgtga
     3121 tcgtggcctc ctcaccattc ctggctttct cggtctgctc ctcgatcagg tgctctatcc
     3181 tggcgcgcaa cgtgatcggg gcaacgagaa gctcctggta ctcctcctgc tcagagtacc
     3241 cggtcaggta gttgaagaga tcggagccgt cctcggcgag gccgggatcg tcggtgaagt
     3301 aagagaaatc cgtgtagacg cgggccgtcg acgggttgta gttgcccgtg ccgaggtgca
     3361 cgtagcgcct gaggccggcg ccctcgcgcc tgacgacgag ggagatcttg gcgtgtgtct
     3421 tgagccccac gatgccgtag gcgacgtgga ctccccttgc ctcgagctgg cgcgcccaga
     3481 tgatgttcgg ctcctcgtcg aaacgcgcct tcagctcgac cagcacggcg acctgcgtct
     3541 gctcgtcgcg cgcctccgag agggcctcca ctatggggga gttggagccg acgcggtaga
     3601 gggtctgctt tatggccagc acctcgggat cgttggccgc cgcccggacc aagtcaacga
     3661 cggggttgaa ggagtcgtag gggtggtaaa ggagcaggtc gccgttcctt atcgcctgcg
     3721 taatggagcg ggcgctccgg atctccggcg gcaccctggg ggtgatcggg gggtacagca
     3781 ggtcggagcg gtcgaggtgc gtgagctcct ccaggtcggc caaccctatg gggtggggcg
     3841 actcgaagac ggagttctcg tcgagcttga gtttgcgcgc cagccagtcc ctcaggtcgt
     3901 cgggcatctc gtctgagacc acaagcctca cgctctggcc gaaccagcgg ctctccaact
     3961 catcctctat ggcctgcaag aggtctgctg cttcgtcttc ctcgatcacg aagtcggcgt
     4021 tgcgcgtgac ctggaatatg tagctcgcta ccacttcttt cccgggaaag agctcgtcca
     4081 ggttcgcgga gattacctcc tcgaccctga cgaagcgcat ctcgggcctc cggccctggc
     4141 cacggggccc gtcgggaagc cgcacgaacc ggtctatcga ggtcggcact tttatgcggg
     4201 ccatcacccg cctgccctcg tttccgatca cgacgagcac gtttaacgag aggttggaga
     4261 tgtgcgggaa ggggtgggcc ggatctatgg cgagcggcgt gaggatgggc agaatctccc
     4321 tgacgaacct ctccctgagc tcccgccgct cggtggcgct gacctcgtgg tagggcacca
     4381 ttattatgcc ctccgcttcg agcgccggca tgagctccgt gttcaatatc ctgcgccgct
     4441 cttcgaagaa ctcctcggtg tggtcgtgga tgcgcaggag ctgctcctct ggcgtcatgc
     4501 cgtcggggac cgggttcggt agctccgagg ccacctgctg ctgcagcccc gcgaccctga
     4561 tcatgaagaa ctcgtcgagg ttggtctccg agatggagat gaagcgtgcc ctctccagga
     4621 gcggatgacg ctcgtcgcga gcctgctcaa ggaccctgtt gttgaacccc agccacgaaa
     4681 gttccctgtt gatgtaaagc gagggatccg agaggcttgt cctgcgcgct acatccgtca
     4741 tatcacttcc ccttcgtcga gcgcaagcag ggtgccttcc ctgattcccc ctcgcgccac
     4801 cgtcagttcc ccttttccga aatgctccag aacagccgcg agcgtggtga ttgcggccgg
     4861 caggaccctg gccctctccg gcgcgagccc gtgctcccgc gcgagcacgc cagaagcccg
     4921 ccccctcgtc tccagggaga gccgccccaa ccggtcggcc gtgagcccgt cgcgggtgat
     4981 cttcaagatc gcgcgcgccg aaccgccgac cgccacgacc tcggcatcgc cgatatccca
     5041 ggcgggcaag agccgcccca cgtgctcccc gagcgccccg agctcgccgt ccgttggagg
     5101 gccttcactc acgaagcgct cggtggtgcg gttggtacca agcggaagcg agacccgctc
     5161 ctcgggaccc ccgcgggcgt cgccgaggat gagctgcgcg gagccgccac cgaggtccac
     5221 gacgagcacc ttcccctcgt gccgcacggc cgagagggcg ccacgaaacc cgagggcggc
     5281 ctccttctcg cccgagatga ggcgcatctc gaggcccgtc gcttcccgta cgcggtccac
     5341 gagttcctgc ccgttgcccg catcgcgcac ggcactggtg gcgagtacct ccggcgagga
     5401 ggtcccgttc atggcggcta tgccgacgaa cagcttgatg gcctcagccg cgaggtcgag
     5461 gcgtcgctcg tcgaggcgtc ccgtcttctc taccccggcc ccgagacggg ccgagacctt
     5521 ctcgcccgct actggaatca cggccccatc atccacctcc ccgacgagca gatgaatggt
     5581 gttggaacca acatcgatga cagaatgccg gctcaatcca tctcccgaga ttgttcgcga
     5641 aacctcatac cacccatcat aacgtcccac cgccccgagt gatatattcg ggtcgtgccc
     5701 acgggttatt agatcgccca ggcccgcgcg tggcgcgggc cggctctaga agcggaggac
     5761 accatctcga agcgaggcaa gaaggccaga aagcacgcgc agaagaacgg cgcgctcgcc
     5821 atcctcgaaa agcccaacgc gaaggttcgc gactacgacg cccacgtgct ggtctgcaag
     5881 ggaggcgact gcaagaaacg cggctcgaaa accgtccgcg gcgcgctgaa ggacgagctg
     5941 cgcgcccagc acatgaaccg ggacgtccgc gtcgactccg tagactgcct cgggctctgc
     6001 aaacacggtc cgaacctcat cgtctatccc ggcggcacct ggtacctggg cgccacggag
     6061 cacgacatcc ccgagatagt tcgagaacac ctggtcaacg gcgaacccgt cgaacacctg
     6121 gccgccgaa
//
LOCUS       DBNGVD010000107         1963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2937763, whole genome shotgun sequence.
ACCESSION   DBNGVD010000107 DBNGVD010000000
VERSION     DBNGVD010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2937763"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="AC16CM_03090"
     CDS             complement(<1..973)
                     /locus_tag="AC16CM_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3138842.1"
                     /translation="MTATARTNLVPSLSEARTLARTYDVVPLYAEFIGDLETPISAVL
                     RFAEEDTVFLLESAESAERFGRYSFLGFDPKRTLSYRDGIYTVVDADGVREVPAKDPF
                     RGLAEIVGKKTVAPLPNLPAFVGGAVGYFSYDAVRYLENLPDAPEDDLNVPEAYFAVT
                     DTLIVFDHLRHKVLVISLVDAAGLRDVEGEGFSAAYRRAADDIRRISERLSAPLLRRP
                     PAFGTGFDISSNFTRERYEEAVEGAKEYIRAGDAFQIVPSQRFAAEIGDLDPLLLYRG
                     LRTVNPSPYMTYLKLGGLSLVGASPEPLVRVEGRRVMTRPVAGTRGRG"
     gene            complement(957..1592)
                     /gene="hisIE"
                     /locus_tag="AC16CM_03095"
     CDS             complement(957..1592)
                     /gene="hisIE"
                     /locus_tag="AC16CM_03095"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3138843.1"
                     /translation="MIPATNRITFDGNGLVPVIAQDAATGSVLTLAYANREAVEKTLD
                     SGEAHYYSRSRSELWRKGATSGNTQKVVEVRLDCDGDALLYVVEPRGPACHTGEESCF
                     FTSLAGEGVAATAESEVRLGTMIERLAATIAQRHEEMPEGSYTVSLIEGGPERLSQKV
                     GEEAVEVVIAALTDDRLSEETADLVYHLLVLLEERGVGTERVAKVLRDRHG"
     gene            complement(1602..>1963)
                     /locus_tag="AC16CM_03100"
     CDS             complement(1602..>1963)
                     /locus_tag="AC16CM_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR3138844.1"
                     /translation="NGGRVNTGMDAVDWLVEGERRGAGEFVLNSMDADGTESGYDLDL
                     ISTVAEKTTLPIVASGGAGGPEHMISAARAGAGAVLAASIFHFGEYSIAEVKERMSRA
                     GIPVRLLEPEREVKLGN"
BASE COUNT          318 a          673 c          633 g          339 t
ORIGIN      
        1 cgccgcggcc gcgggtaccg gcgacgggcc tggtcatgac ccggcgcccc tcgacccgga
       61 ccaggggttc aggtgaggcc ccgaccaggg agagacctcc cagcttcagg taggtcatgt
      121 agggcgatgg gtttaccgta cgcaggccgc ggtagagaag cagggggtcg aggtccccga
      181 tctcggcggc aaacctctgg gagggcacga tctggaacgc gtcgcccgcc ctgatgtact
      241 ccttggcgcc ctccaccgcc tcctcgtagc gctccctggt gaagttggag gagatgtcga
      301 accccgtgcc gaaggccggc ggccggcgca ggagcggggc cgagagacgc tcagagatcc
      361 tgcgtatgtc atctgcggcc cgcctgtagg ccgcggagaa cccctcgccc tccacgtcgc
      421 gcaggccggc ggcatcgacc agcgagatga ccagcacctt gtgcctgagg tgatcgaaga
      481 cgatcagggt atccgtgacg gcgaagtagg cctcaggaac attcaggtcg tcctctgggg
      541 catcaggtaa gttttcgagg taccggactg catcgtagga gaaataccca acagcccccc
      601 cgacgaaggc cggcaggttc gggagcgggg ccacggtctt cttgccaact atctcggcca
      661 ggcccctgaa ggggtctttc gcgggaacct cccgcacgcc gtcggcgtct acgacggtgt
      721 agatgccgtc tctgtaggag agcgtgcgct tggggtcgaa gccgaggaac gagtagcgtc
      781 cgaacctctc ggccgactcg gcgctctcca gaaggaagac cgtgtcctcc tcggcgaacc
      841 tgaggaccgc cgatatgggc gtctcgaggt cgcctatgaa ctccgcgtac agcggcacca
      901 catcgtaggt gcgggcgagc gtgcgcgcct ctgagagcga agggacgagg ttcgtcctag
      961 ccgtggcggt cacgcagcac cttcgccacc cgttccgtcc cgacaccgcg ctcctccagc
     1021 aaaaccagga ggtggtagac gaggtcggcg gtctcctcgg agagcctatc gtccgtcagg
     1081 gccgcgatga cgacctccac ggcctcctcc cccaccttct gggacaatcg ctctggcccg
     1141 ccctcgatca gggacaccgt gtacgatccc tcgggcatct cctcgtggcg ttgcgctatg
     1201 gtcgccgcca gacgctcgat catcgttccg aggcggacct cgctctctgc ggtggccgcc
     1261 accccttccc cggcgaggct ggtaaagaaa cagctttcct caccggtatg gcaggccggg
     1321 ccccggggct cgaccacgta gaggagggca tccccgtcgc aatccagcct gacctcgacc
     1381 accttctgcg tgttgccgct cgtcgcgccc ttccgccaga gctcggagcg cgagcgggaa
     1441 tagtagtggg cctcgccgct gtcgagcgtc ttctcgacgg cctcgcggtt cgcgtaagcg
     1501 agcgtgagca cgctccccgt cgcagcgtcc tgggcgatga cgggcaccag gccgttccca
     1561 tcgaacgtta tcctgttcgt ggcaggtatc atggtttcgg cctagttgcc aagcttgacc
     1621 tcccgttcgg gttccaggag ccgcactggg atgccggccc ggctcatacg ctccttgacc
     1681 tcggctatgc tgtactcgcc gaaatgaaag atgctcgccg cgagcacggc accggccccc
     1741 gccctcgcgg ccgagatcat gtgttcgggg cctccggccc cgccggaggc gactatgggc
     1801 aacgtggtct tctcggcgac ggtggagatc aggtcaaggt cgtagccgct ctcggtccca
     1861 tcggcgtcca tgctgttgag gacgaactca cctgcccccc gcctctcacc ctcgacgagc
     1921 caatcgacgg cgtccatgcc cgtgttgacc cgtccgccgt tga
//
LOCUS       DBNGVD010000108        10385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_387577, whole genome shotgun sequence.
ACCESSION   DBNGVD010000108 DBNGVD010000000
VERSION     DBNGVD010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_387577"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16CM_03105"
     CDS             complement(<1..590)
                     /locus_tag="AC16CM_03105"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HIR3138845.1"
                     /translation="MRTKPYESWTREDKRTRYYGSMWRPNRGMLVLAGLALILVSAGV
                     VFLDIRQAGDEAGAPPPKKDTTMELTIPSLRRVQNVPVYTGTAGDKSALHDGTLHLKG
                     TSFPWQQQNSNVYIAGHRLGYPNTKSWLVFWDLNELKRGSRVILTDSDGTRYIYSVYE
                     RFILGPRDTSATKPVAGMNVISLQTCTLPNYTDRLIV"
     gene            complement(658..1566)
                     /locus_tag="AC16CM_03110"
     CDS             complement(658..1566)
                     /locus_tag="AC16CM_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138846.1"
                     /translation="MAEASAQQDVIAAPAGVRTRSRWQDFVRTFFSNRLAVVGLVVLS
                     LFILMAIFAPLIAPYDPLAQDLEGKFAGPSLEHPFGQDELGRDILSRVIYGARISLTA
                     GLAAVALATVVGTIIGLLAGYFGGYTDSGLMRLMDVILAFPSILLAIIIVSVLGPSLA
                     NAMLAIGIVFIPQIARVVRSAVISVRERDYIEAERALGASNSQIIVSGILPNSMAPLI
                     VQATLTLATAILDIAALSFLGLGAQAPTPEWGAMLTDAFKSGFGVFVEGQHALIFPGI
                     AIALAVLSVNFVGDGLRDALDPRRRG"
     gene            complement(1570..2571)
                     /locus_tag="AC16CM_03115"
     CDS             complement(1570..2571)
                     /locus_tag="AC16CM_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3138847.1"
                     /translation="MAEYVGRRIFQIIPVLLGVSIVVFCMVRAIPGDPAQIMLGQTAT
                     EAQVAEVRNRLGLDEPLPVQYLVFLKGAVRGDLGDSIVTGRPVVNELLVRLPATLELT
                     GFAMIIAILVGVPVGVISAVRQYSVLDKVASTTALTGISMPIFWLAMILIVIFGVNLE
                     LLPFPGRLGPTTAITSITGLVLVDSLLTLNFAGFWDGLLHLIMPAIALGTIPMAVIMR
                     MTRSSMLEVMGEDYVRTARAKGVAPWRVVFKHSLRNAMLPTVTVIGLSAGLLMGGSII
                     TETIFSWPGVGQIAYESVNRRDYAMIQGVVLYGAALFVFINLAVDVLYAVLDPRVRL"
     gene            complement(2616..4316)
                     /locus_tag="AC16CM_03120"
     CDS             complement(2616..4316)
                     /locus_tag="AC16CM_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3138848.1"
                     /translation="MARTGEGNKISRGDFLRLSGAGLTGVTLLGLAGCGGGGTISGGG
                     GSSGGSTLTFGRGADSVTLDPVNATDGESFRVTRQIFDTLLDFAPESFDLQPSLASEI
                     PKPENGGRTYTLKLRPGIKFHDGTTLDAEAVKFNFDRWRDTKNEYHKGGGSQTSEFSY
                     YSSQFGGFDDDSVIESVEVVDENTVRFNLKEPQGPFLRNLTMSPFAIASPKAIQKDVD
                     GFWQNPVGSGPYTFVSWERNSQVRVEAFKDWWGSDLPASEGFGGPKMKTVIIRSIPEN
                     TARVAALTGNQLNMADGLVPDDVPTIEEQEGFKLDFRPPNTIGYLAMNNEKKPFDDPR
                     VRQAFNYAIDVPKIVETVLRGTGEVASNYMPSLLPYFNKSVEPYPYDPQKAKQLLKQA
                     GVSNLSTELWYMPIPRPYMPDAKSMAQVMQQDLKKVGVNAKLVTYEWGTYLEKTGEGA
                     HPMCLLGWTGDNGDPDNFLNVLLNSATATPTDALNVAYYKNPEVDKLLVQGQTTVDEN
                     ERKKVYFRAQEIMHEDAPWVPVAYVEPPIGLQQSVQGFAPNATSSEALNTVFISGGGK
                     "
     gene            complement(4425..5123)
                     /gene="nfi"
                     /locus_tag="AC16CM_03125"
     CDS             complement(4425..5123)
                     /gene="nfi"
                     /locus_tag="AC16CM_03125"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010045.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR3138849.1"
                     /translation="MKVSGLHGLDLSPSEARRLQENLASRVVAGPVLDLTGVRHVAGA
                     DVSTQGDMAYGTVVVLDFPGLTPVEVQGFREQLRFPYVPGLLSFRELPSVVGALERVQ
                     TQVDALILDAHGLAHPRRMGLASHLGIFLDVPTVGCAKSLLVGSFDEPGREKGSATDL
                     VHRGEVVGKVLRTRDGVSPVYVSVGNGIDLGSSVELVLACCPRYRLPETTRQAHNAAN
                     RLRKESEGEAPLAY"
     gene            complement(5482..7161)
                     /locus_tag="AC16CM_03130"
     CDS             complement(5482..7161)
                     /locus_tag="AC16CM_03130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR3138850.1"
                     /translation="MSKQTTNRMRAGVAGVIGAVVAFGIAELVHGLYPPVPSIFVSLA
                     QRIVELTPGTLVTRGIELLGTADIPTLIVCLLLGTAVVAALLGNLAVGRPSLASLSVV
                     VLAAVAVAAALADPFVEALPTVITIVVALLAGSAITELLLRAAGLRPTPEGAGEPLPA
                     GAPGSALSPAARSREAGPGGETSVGRGGFLLLGGGAVVAGLAAAGIGRLLAGGAQEVA
                     GKPRRLSLPDSPGKNDGAQGSAGRAATHDTLPRPPADASINVPGMPDLITPTSSFYLI
                     DTALVSPRIDVNNWKLSVKGAVQNPVEFSYKDLLGMPTREADITLSCVSNTVGGGLVS
                     NGRWTGVLLSDVLAEAGVSREEITSASRQLVGRSVDGFTTGFKTDIALDGRNAIVAFG
                     LNGSELPTKHGYPVRLVIPGLYGYVSATKWLTELELTDWDFDAYWIQRTWSKEGPVKT
                     QSRIDTVKDGDNLSPGKNPIGGIAWAPHRGIERVEVSTDGGDTWNTARLARQLAEDTW
                     RQYVYDWDATPGDHTIQVRATDGTGETQTAAEAPPHPSGATGYHTINVSVG"
     gene            7655..>10385
                     /gene="ileS"
                     /locus_tag="AC16CM_03135"
     CDS             7655..>10385
                     /gene="ileS"
                     /locus_tag="AC16CM_03135"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3138851.1"
                     /translation="MKFDKVSPQVDFPRLEEGVMGLWQETDAFHESVRRRPADRPYVF
                     YEGPPTANGKPGFHHALSRAFKDLIPRYKTMRGYRVERKGGWDCHGLPVEIGVERELG
                     LNGKEDIERYGVEEFNRLCRESVFRYVGDWQKMSERLGFWVDMENPYRTLDDSYIASV
                     WWALKTLHDKDLLYEGFKVSPHCPRDQTSLSSAEVALGYQQYPEPVVDPSVYVRLPLR
                     DEENISLLIWTTTPWTLISNTAVAANPDVDYVTVRVGEERLILARDLLEKALGEETDY
                     EVLETRKGAALEGLRYKRPFDYVPVEETENLWSVVVDGYVTTTEGTGLVHTAPAYGED
                     DARLGRKYGLPTVHPVRPDGTFDERVGPFAGRFVKDADPGLTEELAENGLLFRAETLE
                     HAYPHCWRCGTPLLYYAKKAWYGRTTAVLDDLLKENEDINWVPEHIKWGRFGDWLENN
                     IDWALSRERYWGTPLPIWRTASGEVVVVGSKEELEELAIDPVPEDLHRPYIDRVRVRH
                     PETGEEATRVPEVLDVWFDSGSMPFAQWGYPHGENSAERFGGQFPADYICEAVDQTRG
                     WFYSLLAVSTMLFGKSSYRTCQVLGHILDAEGKKMSKSLGNVIDPWEVFEKQGADALR
                     WALYTATSPGNTRRFSMGQVDEAVRKYLLTLWNTYSFFVTYARIDGFDPREDYVEPGD
                     RSLMDRWALSELQLTVRTVTERLDAYDVTAAGRAIGEFVDELSNWYVRRSRRRFWKGE
                     DDRDKKAAHSTLYECLVTITKLTAPFTPFVAESLYQNLVADSEGRESVHLAHWPEHEA
                     GLVDVGLSDRMAAARRVVGLGRAARNAAAIKTRQPLREVVVVDEGSDGSSLREGVQNL
                     REIVLDELNVKELAFGEAEDVFVYDLKPDLGVVGPKYGRLVPGIRAALDAAPAE"
BASE COUNT         1885 a         3270 c         3472 g         1758 t
ORIGIN      
        1 acgatcaggc ggtccgtgta gttcgggagc gtgcaggtct ggaggctgat cacgttcatc
       61 cccgcgaccg gcttcgtggc cgaggtgtcg cgggggccaa gaatgaaccg ctcgtagacg
      121 ctgtagatgt acctggtgcc atccgagtcg gtcaggatca ccctgcttcc gcgcttcaac
      181 tcgttgaggt cccagaacac cagccagctc ttggtgttcg ggtagcccag cctgtggccc
      241 gctatgtaga cgttcgagtt ctgttgctgc caggggaaac tcgtcccctt caggtgcaac
      301 gtcccgtcgt gcaacgccga cttgtcgccc gcggtgccag tgtagacggg gacgttctga
      361 acccgcctca ggctcgggat ggtcagctcc atggtggtgt ccttcttcgg cggtggcgcg
      421 cccgcctcat cccccgcctg cctgatatcc aggaaaacga cgccggcgga gaccagtatc
      481 agggccagcc cagccagcac gagcatgcca cggttaggcc tccacatgga accgtaatag
      541 cgcgtcctct tgtcttcgcg ggtccaagac tcgtacggtt tcgtcctcac gacgaaatat
      601 catacagtcc gcgggcgaca aaacaacgcg gagcaccacc ggcggcgccc atgcccgcta
      661 ccctctgcgg cgggggtcaa gggcgtcgcg cagcccgtct cctacgaagt tgaccgagag
      721 cacggccagg gcaatagcga tgccggggaa gatcagggcg tgctggccct cgacgaagac
      781 gccaaagccg ctcttgaagg cgtcggtgag catcgcgccc cactcgggag tcggggcctg
      841 ggcaccgagg cccaggaagg agagagccgc aatatccagg atagcggtgg ccagcgtgag
      901 cgtggcctgc acgatgaggg gggccatgct gttcggtagt atgccggaga cgatgatctg
      961 gctgttcgag gcgccgaggg cccgctcggc ctctatgtag tcccgctcac gcacgcttat
     1021 gacggccgag cgcaccacgc gggctatctg gggaataaac acgatgccta tggccagcat
     1081 cgcgttggcc agggagggac ctagtacgct aacgatgatg atcgccagca agatgctcgg
     1141 gaaggccagt atcacgtcca taagccgcat caggcccgag tcggtgtaac cgccgaagta
     1201 gccggcgagc agccctatga tggtaccgac gaccgtcgcg agggccacgg cggcgaggcc
     1261 cgcggtcagc gagatcctgg cgccgtagat cacgcgcgag aggatgtccc tcccgagctc
     1321 gtcctgcccg aacgggtgct cgaggctggg cccggcgaac ttgccttcga gatcctgcgc
     1381 cagtgggtca tagggcgcga tgaggggggc gaagatggcc ataagtatga acaggcttag
     1441 gacgacgagc ccgacgaccg cgaggcgatt ggagaagaag gtcctgacga agtcctgcca
     1501 acggctgcgc gtacggacgc ctgccggtgc ggctatgacg tcttgctggg cgcttgcctc
     1561 tgccatgcct tacaacctca ccctcgggtc gaggacggcg tagagcacgt ccacggcgag
     1621 gtttatgaag acgaagagcg ccgccccgta gaggaccacg ccctgtatca tggcgtagtc
     1681 gcgacggttg acggactcgt aggcgatctg gcccacccca ggccacgaga agatggtctc
     1741 cgtgatgatc gaccctccca tcaagagccc ggccgaaagg cctatgaccg tgaccgtggg
     1801 aagcatagcg ttcctcaggg agtgcttgaa gaccacccgc cacggcgcta cgcccttggc
     1861 ccgggccgtc ctcacgtaat cctcccccat cacctccagc atgctggagc gcgtcatccg
     1921 catgatcacg gccatcggta tggtgccgag ggctatggcc ggcatgatga ggtgcagcag
     1981 gccatcccag aaaccggcaa agttgagggt caggagcgag tccaccagca cgagacctgt
     2041 tatggaggtt atggccgtgg taggaccaag ccgcccgggg aacggtagca actccaggtt
     2101 cacgccgaag atgacgataa ggatcatggc gagccagaag atgggcatgc ttatccctgt
     2161 aagggcggtg gtcgaggcga ccttgtccag gaccgagtac tgcctgacgg cgcttatcac
     2221 ccctacaggc accccaacaa ggatcgcgat gatcatggcg aagcccgtga gttcgagggt
     2281 ggcaggcaga cgcacgagga gctcgttgac cacggggcgg cccgtcacga tcgaatcacc
     2341 gaggtctccc ctgacggcgc ctttcaggaa tacgagatac tgaaccggca ggggctcgtc
     2401 aagcccgagg cggttcctga cctcggccac ctgcgcctcc gtcgcggtct gccccagcat
     2461 gatctgagcg ggatcccccg ggatggccct gaccatgcag aacaccacga tggagacccc
     2521 gagcaagacc gggatgatct ggaagatcct gcgtcctacg tactcagcca gcgccgggac
     2581 ctccgtccga gcggtgagtt cctgccctca ccgccctact taccgccgcc ggagatgaag
     2641 accgtattca gcgcctcgct gctcgtggcg ttgggggcga agccctgcac ggactgctgc
     2701 aggcctatgg gcggctcgac gtaggcgacg ggcacccagg gggcgtcctc gtgcatgatc
     2761 tcctgcgccc tgaagtagac cttcttgcgc tcgttttcgt cgacggtcgt ctggccctgt
     2821 acaagcagct tgtcgacctc ggggttcttg taataggcga cgttcaaagc atccgtcggg
     2881 gtggcggtcg cgctgttgag cagcacgttg aggaagttgt cggggtcgcc gttatcgccc
     2941 gtccacccca gaaggcacat ggggtgtgcc ccctcgccgg tcttctccag gtacgtaccc
     3001 cactcgtagg tcaccagctt ggcgttgacg ccgacctttt tgaggtcttg ctgcatgacc
     3061 tgcgccatgc tcttggcatc gggcatgtac gggcgcggta tgggcatgta ccacaactcg
     3121 gtgctcaggt tggaaacgcc ggcctgtttg agcaactgct tggccttctg ggggtcgtat
     3181 gggtagggtt cgacgctctt gttgaagtaa ggcagcaacg agggcatgta gttggaggcg
     3241 acctcgcccg tacccctgag gacggtctcc acgatcttgg gaacgtcgat ggcgtagttg
     3301 aaggcctgcc tgaccctcgg gtcgtcgaac ggcttcttct cgttgttcat cgccaggtag
     3361 ccaatggtgt tgggcggcct gaagtccagc ttgaaccctt cctgctcctc gatggtaggc
     3421 acgtcgtcgg ggacgagacc gtcggccatg ttgagctggt tgcccgtgag cgcagcgacc
     3481 ctggcggtgt tctctggtat ggagcggatg atcacggtct tcatcttggg accgccgaat
     3541 ccctcgctcg cgggcaagtc ggagccccac cagtccttga aggcctccac gcgcacctgg
     3601 gagttcctct cccagctgac gaacgtgtag ggcccgctcc ccacggggtt ctgccagaag
     3661 ccatcgacgt ccttctgtat ggccttggga gaagctatgg cgaacggcga catggtcagg
     3721 ttcctcagga aaggcccctg gggctccttg aggttgaagc gcacggtgtt ttcgtctacg
     3781 acctcgacgc tctctattac ggaatcgtcg tcgaagcccc cgaactggga ggagtagtag
     3841 gagaactccg aggtctggct gccgccgccc ttgtggtact cgttcttggt gtcgcgccag
     3901 cggtcgaagt tgaacttgac cgcctcggcg tcgagggtag tgccgtcgtg aaacttgatg
     3961 ccgggccgca gcttgagagt gtaggtgcgc ccaccatttt cgggcttggg gatctcgctg
     4021 gccaacgacg gctgcagatc gaagctctcc ggcgcaaagt ccaggagcgt gtcgaagatc
     4081 tggcgcgtca cccggaagga ttcgccgtcg gtggcgttga cggggtccag cgtgacggag
     4141 tcggccccac gcccgaaggt gagcgtgctg ccaccagagc tacctccacc cccgcttatg
     4201 gtcccgccgc caccgcaccc tgccaggccg agcagcgtca caccggtcag gccggcgccg
     4261 ctcaaccgca ggaagtcgcc gcggcttatc ttgttgcctt cgcccgtgcg tgccaagttg
     4321 gcccctttcc ctgttgttaa tcccttgcgc acggtccacg cgggaccggt acggctagtc
     4381 ttgcacccct cgtaaactaa cattccttcc gggccgacgc taggctagta ggccaacggc
     4441 gcttcccctt cggactcctt cctgagcctg ttggccgcgt tgtgcgcctg ccgcgtggtc
     4501 tcgggcagcc tgtacctggg acagcacgcc aggacgagct ccacggaact ccctagatct
     4561 atgccgttcc cgaccgagac gtagacgggc gagaccccgt ccctggtcct caacaccttg
     4621 cccacgacct cgccgcgatg aacgagatcg gtggcactgc ccttctccct cccaggttca
     4681 tcgaagcttc ccaccagcag gctcttggca cacccgaccg taggcacgtc gaggaaaatc
     4741 cccagatgcg aggcaagccc catccgccgg ggatgggcga gcccatgagc atctagtatg
     4801 agcgcgtcga cctgcgtctg gacacgctcc agggctccga cgacggacgg taactctcgg
     4861 aacgagagca gcccgggaac gtaggggaac cggagctgct cccggaagcc ctgcacctcg
     4921 acgggcgtga gcccggggaa atccagcacg acgaccgtgc cataggccat gtctccctgc
     4981 gtggacacgt cagccccggc cacgtgacgc accccggtga gatctagcac cggtccggcc
     5041 accacgcgag acgccagatt ctcctgcaac cgcctcgcct cggagggcga gagatccagg
     5101 ccgtgtaacc cgcttacctt cataaggtta cgttaacaaa tgtgtaggcc agaccaggaa
     5161 actgggagat cagaagtaac ataccccaca aaaacaccgt cccgaccgat ggaatctgga
     5221 ggggggaacg gttccatcgg ccggggctcg gttcaatctg aatcttaccc ttagcctgtt
     5281 acgaaacgtt tgccgtgttg ttacggtatc gtaacgtaac ggaaacactt tgattacaga
     5341 actgtatcgg tgcaacagaa ttcgtattct agccggtacg agcctgtatt ccaggcggca
     5401 tatgtttaac cctgaactta taggtgaggg cggtttgcgg gaccgggcgg gttccaaacc
     5461 cgcccccaca cgggcacggc gctagcctac ggagacgttt atcgtgtggt agccggtggc
     5521 acccgagggg tgcggggggg cttccgcggc ggtctgggtt tcgccggtgc cgtcggtggc
     5581 gcgcacctgg atggtatggt cgcctggggt ggcgtcccag tcgtagacgt actggcgcca
     5641 ggtatcctcg gcaagttgcc tggccaggcg ggccgtgttc caggtatcgc cgccgtctgt
     5701 ggagacctcc accctctcga tgccgcggtg tggagcccag gcgatacccc cgatggggtt
     5761 cttgcccggg gagaggttat cgccgtcctt gacggtatcg atgcgagact gggtcttcac
     5821 gggaccttcc ttcgaccagg tgcgttggat ccagtacgcg tcgaagtccc aatccgtcag
     5881 ctccagctcg gtgagccact tcgtggcaga gacgtagccg tagaggccgg ggatcaccag
     5941 ccgtacgggg tatccgtgct tggtaggcag ctcgctgccg ttcagcccaa aggcaacgat
     6001 agcgttgcgc ccgtccaggg cgatatccgt cttgaagccc gtcgtgaagc catccacgct
     6061 gcgccccacc agttgcctgg aggcggaggt gatttcttcc cggctcacgc cagcttcggc
     6121 gagaacgtcg gagaggagaa cgccggtcca gcgcccgttg gagaccagcc cgccgccgac
     6181 agtgttcgat acgcaagaga gggtgatgtc tgcctcgcgg gtgggcatgc cgaggaggtc
     6241 cttgtatgag aactcaaccg ggttctgtac ggcgcccttc accgagagct tccagttgtt
     6301 cacgtctatc cttggggaga ccagcgccgt atcgatcagg tagaagctgg atgtcggggt
     6361 gatcagatcc ggcatccctg gcacgttgat ggaggcgtcg gccgggggcc ttggaagcgt
     6421 gtcgtgggtg gcggcccggc ccgcactccc ctgcgcccca tcgttcttcc ccggtgagtc
     6481 aggcaggctc aacctcctgg gcttgccggc gacctcctgg gcgccgccgg ccagaagacg
     6541 cccgatgccg gccgcggcga ggcctgccac gacggcgccg ccaccgagca gaaggaaacc
     6601 gccacggccc acggaagtct ccccaccggg gccagcctcg cgggacctgg cggccggtga
     6661 gagcgcgctg cccggcgcgc cggccggaag gggctcgccc gctccctcag gggttggacg
     6721 gaggccggcg gcccgcagga ggagttcagt gatggccgag ccggcgagta gggccacgac
     6781 gatggtgata acggtaggca acgcctctac gaacgggtcg gcgagggccg cggccacggc
     6841 tacggcggcc aggacgacaa cgctgaggga ggcgagggag ggacgcccga cggcgaggtt
     6901 gccgaggagg gcggccacga ccgcggtgcc aagcagcagg cagacgatca gggtcggtat
     6961 gtcggccgta ccgaggagct cgatacccct ggtgacgagg gtacccggtg tcaactccac
     7021 gatgcgctgg gccagcgaga cgaagatcga gggtacgggc gggtaaaggc cgtgcacgag
     7081 ctcagcgatc ccgaacgcca ccacggcccc aattacgccg gcgacgccgg ccctcatcct
     7141 gtttgtggtc tgcttgctca tatcggtgct ctccaatgcc tctgtgaggt gctttcctga
     7201 gtctataccc gggacggccg gcccgaaccg aaagccatca caagtactta cggatcatct
     7261 ctcgggaccg gatcaacccg acttgacacc ggcgtgccgt ggcgctacaa aaccctctac
     7321 aggcgacgaa gggaacgagt agggcctgga gcaacccgca agagcgagcc ggggacggtg
     7381 cgagcccggc gcgggagatg gtccgaacat cctccccgag ccgccggccg aaagcggaag
     7441 gcagagactg cctagacgat tagtaggacc ggccgggtgg gcccgttaca gcgcagtcgt
     7501 tgcgggggac cgcgacgaga cgagaggacc ggaccgcgag gtcaggggca agtagggtgg
     7561 taccgcgagg gcctagagtc gaggctcccg tccctacggt gtggagcggc gctccgccgg
     7621 gggcgggagc cttttgtatg aggaggtccg aggtatgaag ttcgacaagg tcagcccgca
     7681 ggtagatttc ccgaggctgg aagagggtgt catgggcctc tggcaggaga ccgatgcctt
     7741 ccacgagtcc gtgcggcgca ggcccgcgga caggccctac gtgttctacg aggggccgcc
     7801 gacggccaac gggaagcccg ggttccacca cgccctctcg cgggccttca aggacctgat
     7861 cccgcgctac aagacgatgc ggggctaccg ggtggagcgg aagggcggct gggattgcca
     7921 cgggttgccc gttgagatag gggtggagcg ggagctcggc ctgaacggca aggaggacat
     7981 cgagcgctac ggcgtcgagg agttcaaccg cctgtgccgc gagtcggtct tccgctacgt
     8041 gggggactgg cagaagatga gcgagcgcct cggcttctgg gtggacatgg agaacccgta
     8101 ccgcacgctt gacgactcgt acatagctag cgtgtggtgg gccctgaaga cgctgcacga
     8161 caaggacctg ctctacgagg gcttcaaggt cagcccgcac tgcccgcgcg accagacctc
     8221 cctctcgtct gcggaggtcg ccctcggcta ccagcagtac cccgagcccg tcgtcgaccc
     8281 gagcgtctac gtcaggctcc cgctccggga cgaggagaac atcagcctcc tcatctggac
     8341 caccacgccg tggaccctca tctccaacac cgccgtggcc gccaaccccg acgttgacta
     8401 cgttaccgtc agggtcggag aggaacgcct gatcctggcc cgcgacctgc ttgagaaggc
     8461 gctcggggag gagaccgact acgaggtgct cgagacccgc aagggcgcgg cccttgaggg
     8521 gctccgctat aagcgtccct tcgactacgt ccccgtggag gagacggaga acctgtggag
     8581 cgttgtcgtg gacggctacg tgaccacgac cgagggcacg ggcctggtcc acaccgcgcc
     8641 ggcctacggc gaggacgacg cccgcctggg ccgcaaatac gggctgccca cggtccaccc
     8701 ggtgaggccg gacggcacgt tcgacgagcg ggtcggcccc ttcgccggcc ggttcgtgaa
     8761 ggacgcggac cccggcctga ccgaagagct cgcggagaat ggccttctgt tcagggcgga
     8821 gacgctggag cacgcctacc cacactgctg gcgttgcggc acgcctttgc tctactacgc
     8881 caagaaggcg tggtacggga ggacgaccgc cgtgctcgac gatctcctga aggagaacga
     8941 ggacatcaac tgggtcccgg agcatataaa gtggggccgc ttcggtgact ggttggagaa
     9001 caacatagac tgggccctga gccgcgagcg ctactgggga acgccgctgc ccatctggcg
     9061 caccgcctcg ggcgaggtgg tagtcgtggg cagcaaggaa gagctggaag agctggccat
     9121 agaccccgtc cccgaggacc tgcacaggcc ttacatagac cgggtgcgcg taaggcaccc
     9181 agagaccggc gaggaggcaa cccgggtgcc agaggtcctc gacgtgtggt tcgactcggg
     9241 gagcatgccc ttcgcccagt ggggctaccc gcacggcgag aacagcgcgg agcgcttcgg
     9301 ggggcagttc ccggcggatt acatctgcga ggccgtcgac cagacgcggg gctggttcta
     9361 ctcgctgctc gccgtctcca ccatgctgtt cggaaagagc tcgtacagga cgtgccaggt
     9421 cctcgggcac atcctggacg ccgagggaaa gaagatgagc aagtcgctcg gcaacgtcat
     9481 agacccctgg gaggtcttcg agaagcaggg cgccgacgcc ctgcgctggg cgctctacac
     9541 ggcaacgagc ccgggcaaca cgcggcgctt ctcgatgggc caggtcgacg aggccgtccg
     9601 caagtatctg ctgaccctct ggaacaccta ctcgttcttc gtcacctacg cccgcataga
     9661 cggtttcgac ccgcgggagg actacgtgga gccgggggac cggagcctga tggaccggtg
     9721 ggccctcagc gagctgcagc tcaccgtgcg taccgttacg gagcggctgg acgcctacga
     9781 cgtaacggcg gcggggaggg ccataggcga gttcgtggac gagctctcca actggtacgt
     9841 caggcgctcg cggaggcgct tctggaaggg cgaggacgac agggacaaga aggcggccca
     9901 ttccacgttg tacgagtgcc tggttaccat caccaaactc accgccccct tcaccccgtt
     9961 cgtcgccgag tccctctacc agaacctggt tgctgacagc gagggtcgag agagcgtgca
    10021 cctcgcccac tggccggagc acgaggcggg gctggtcgac gtcggcctct ccgacaggat
    10081 ggcggcggcg cggcgcgtgg tcgggctcgg cagggccgcg cgcaacgccg cagcaataaa
    10141 gacgcgccag ccccttcggg aggtcgtcgt ggtggacgag ggttcggacg ggtcgtcgct
    10201 cagggaaggc gtccagaacc tgcgggagat cgtgctagac gagctaaacg tcaaggagct
    10261 cgccttcggg gaggccgagg acgtgttcgt ctacgacctg aagcccgacc ttggcgtcgt
    10321 cggcccgaag tacggaaggc tcgtgcccgg cataagggcg gcgctcgacg cggcccccgc
    10381 cgagg
//
LOCUS       DBNGVD010000109         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_205106, whole genome shotgun sequence.
ACCESSION   DBNGVD010000109 DBNGVD010000000
VERSION     DBNGVD010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_205106"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="AC16CM_03140"
     CDS             complement(<1..565)
                     /locus_tag="AC16CM_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3138852.1"
                     /translation="MPDEGFRVEPAEAGERLDRLAARRLGISRSLVQRLIGEGDLRVD
                     GEESYPSFKVRGGQLVEALLPEEGISAEDIPVPVVFEDEHVLVVDKPAGLVVHPGAGN
                     PSGTLVNALLARGIAGGEDPSRPGVVHRLDRDTSGLMVLARGEPAYSRLVEMMANRQV
                     ERLYRAVVVGEGLPETGTIDSPVGRDPD"
     gene            complement(634..1773)
                     /locus_tag="AC16CM_03145"
     CDS             complement(634..1773)
                     /locus_tag="AC16CM_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR3138853.1"
                     /translation="MPIRPIDIRRKEFKNGFRGYDANQVDDFLDAVADEYERNYTENQ
                     RMREEISSLRDRLQQFEDLEGSIRAALVHAEQAANDLRRAASSEAEDMRQSAQREADF
                     TIKEAQTRSHQMLADSSSRVERVQESYEALQEAKKSFTNDFRHLLKTYTDVMESMETA
                     SARQIEASLRERLDTESIAAAHEAALQDPRPTEEPDVGVPDEDEYTDPGLAVSEQEIA
                     ESEEEAVVPVEPDEVDPDATMRIETGTLVPEEPISEEELAAADVVPEQSEAQRHEGPL
                     ADEQPTEVLRAEDLGPFARAPGEPPAEEAAPSTVEDETQQFEESPVEGEPASEPAEEV
                     EASQNETPLAEENPETSPEGSPESEQAEQGDSRIFRASRFLRRRD"
     gene            complement(1778..2113)
                     /locus_tag="AC16CM_03150"
     CDS             complement(1778..2113)
                     /locus_tag="AC16CM_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3138854.1"
                     /translation="MALLQEFGFSVAALLSGVVTYTYYILFGFIIASILFSWFPGYPS
                     SSFMQAVYDAVRAVANPILMPIRSRVPMLQLGGFGIDLSPIIAILGLSIARTLLLIII
                     ENFIQPVTG"
     gene            complement(2113..2898)
                     /gene="proC"
                     /locus_tag="AC16CM_03155"
     CDS             complement(2113..2898)
                     /gene="proC"
                     /locus_tag="AC16CM_03155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3138855.1"
                     /translation="MQKVGIIGAGKIGGSLLLRLAESDGFDLGVSDIHPEQLGHFRDL
                     GIHTTSSNRELAEQSELLIVAVKPWDVAQVMREISDSFEGTDKAVASVAAGVTIASIA
                     ESLPPGTAILRVMPNVCASVGLGSAVVTANEAGRSALPTVVDIFRHVGEVQELPERLF
                     DAATALHGSGPAYVALFADALIQAGVREGIPRDVARKLVIGTIGGTAVLLKERSAHQV
                     RDEVMTPGGTTAAAFVAMEKAGFVGAVYDGVEAATEQARGLGG"
     gene            complement(2906..3484)
                     /locus_tag="AC16CM_03160"
     CDS             complement(2906..3484)
                     /locus_tag="AC16CM_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR3138856.1"
                     /translation="MGVRDRLDRIAAWFGFGVDEDDYYEDEEDMDRRYDDDEYSNGAR
                     GSEPGPTVRRLGRAERSSAFGTSLGDLFGSDSSARGDRLSANQNPPAHLRPVPDQRTT
                     RVSVLEPASFNDAQNLADRFKRQQPVILNLQNVDGDLSRRMVDFCSGLTYALDGNIQS
                     VANRVFLLTPRDVEVSAEERKRIAERAFFNQL"
     gene            complement(3474..>3665)
                     /locus_tag="AC16CM_03165"
     CDS             complement(3474..>3665)
                     /locus_tag="AC16CM_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138857.1"
                     /translation="VFAKLRAIRDRLRESWSPHFDLSELSMGMSGDYEVAVEEGATVV
                     RIGRMLIEEDGPVRRQDGS"
BASE COUNT          566 a         1241 c         1168 g          690 t
ORIGIN      
        1 tgtcggggtc gcggcctacg ggcgagtcga tggtcccggt ctcgggcagc ccctccccca
       61 caacgaccgc cctgtacagg cgctcgacct gccggttcgc catcatctct accagccgcg
      121 agtacgcagg ctcgcccctg gccagcacca tgaggcccga ggtgtcacgg tcgagacgat
      181 gcacgacgcc tggcctggac gggtcctctc cccccgctat gcccctggcc aggagcgcgt
      241 tgacgagcgt gcccgacggg ttgccggcac cggggtggac gacgagacct gccggtttgt
      301 ccacaacaag gacgtgctcg tcttcgaaga ccacgggcac ggggatgtcc tccgccgaga
      361 tcccttcttc gggtagcagg gcctcgacga gttgaccgcc cctgaccttg aaggaggggt
      421 aactctcttc gccgtcgacg cgcaggtcgc cctccccgat caggcgctgc acgaggctgc
      481 ggctgattcc gagacgccgc gccgccagcc ggtcgagccg ctcaccagcc tcggcgggtt
      541 cgaccctgaa gccctcgtcc ggcatcctgc tagaaaacgt ggccggtcag gctcgtgcgt
      601 gggccgccga ctctagtacg ggtggggaac ccctcaatca cgccgacgca ggaagcggct
      661 ggccctgaat atgcgactgt cgccctgctc ggcctgttcg ctttccgggg acccctccgg
      721 gcttgtctcc gggttctcct ccgcgagtgg ggtttcgttc tggctcgcct ctacctcctc
      781 cgccggctcg gacgcgggct ctccctccac gggagattcc tcgaattgtt gcgtctcgtc
      841 ctcgaccgtg gacggggcgg cctcttcggc ggggggctcc cccggcgccc tggcgaaggg
      901 gccgaggtct tcggcccgaa gcacctccgt tggctgctcg tccgcgagcg gtccctcatg
      961 ccgctgcgcc tctgattgct cgggcacgac gtcggcggcg gcgagctcct cctcgcttat
     1021 gggctcttcg ggaaccaggg tacccgtctc gatacgcatg gtggcatccg ggtcgacctc
     1081 gtccggctcc accgggacta cggcctcctc ttcggactcg gcgatttcct gctctgagac
     1141 ggcgaggccg gggtcggtgt attcgtcctc atccggaacg ccgacgtcgg gttcctcggt
     1201 tggccgcggg tcctgcagcg cggcctcgtg ggccgcggct atggactcgg tgtcgagccg
     1261 ctcgcgcagg gaggcctcta tctgccgggc cgaggcggtc tccatgctct ccatcacgtc
     1321 cgtgtaggtc ttcaggagat gtcggaagtc gtttgtaaag ctcttcttgg cctcctgcag
     1381 ggcctcgtag gattcctgaa cacgctcgac ccgcgaggat gagtcggcca gcatctggtg
     1441 ggagcgcgtc tgggcctcct ttatggtgaa gtccgcctcg cgctgggcgc tctggcgcat
     1501 atcctcggcc tcgctgctcg ctgcgcgcct caggtcgttg gcggcctgtt cggcgtggac
     1561 cagggcggcg cgaatagaac cttccaggtc ctcgaactgc tgcagcctgt cgcggaggct
     1621 cgagatctcc tcgcgcatcc tctggttctc cgtgtagttg cgctcgtact cgtcggcgac
     1681 agcatccaga aagtcgtcga cctggttcgc gtcgtagcca cgaaaaccgt tcttgaactc
     1741 cttgcgtctt atgtcgatcg gccgaatcgg catctcatca ccccgtaact ggttgaatga
     1801 aattctcgat aattatcaag agcaaggttc tggctataga caggccgagg atcgcgatta
     1861 tcggtgagag gtctatgccg aacccaccga gttgcaacat aggcacgcgg ctcctgatgg
     1921 gcatcagtat cgggttggca accgccctca cggcatcgta gaccgcttgc ataaagctgc
     1981 tcgaagggta tccggggaac caggagaaga gtatcgaggc gataatgaac ccgaacagga
     2041 tgtagtaggt gtaggtcacg accccagaca gaagtgcggc cacgctgaac ccgaactcct
     2101 gcaaaagggc catcacccgc caagccccct ggcctgctcc gtcgccgcct caaccccgtc
     2161 gtagacggcc ccgacaaacc cggccttctc catggcaacg aacgcggcgg ccgtggtgcc
     2221 gcctggcgtc atgacctcgt cgcgcacctg gtgggcgctg cgttccttga gcagcaccgc
     2281 cgtgcccccg atcgtgccga tcaccagctt gcgggcgacg tcgcgcggta tgccctccct
     2341 gaccccggcc tgtatgaggg cgtccgcgaa gagggcgacg tacgccggcc ccgaaccgtg
     2401 cagggccgtg gcggcgtcga agaggcgctc aggaagctcc tggacctcgc cgacgtggcg
     2461 gaagatatcc acgaccgtcg ggagcgccga gcggccggcc tcgttggcgg ttaccacggc
     2521 tgaaccgagg ccaacggagg cacacacgtt gggcatgacg cgcaggatcg ccgttccagg
     2581 gggcaggctc tcggcgatgc tagcgatggt gacgcctgcg gccacgctcg cgacggcctt
     2641 gtccgtgccc tcgaaggaat cggagatctc ccgcatcacc tgcgccacgt cccagggttt
     2701 cacggcgacg atcaggagct cgctctgctc ggcgagctcg cggtttgagg aggtggtatg
     2761 gatgcccagg tccctgaagt ggcccagctg ttcggggtgg atgtcgctta cgccgaggtc
     2821 gaaaccgtct gactcggcga gcctcaggag gagggagccc ccgatcttgc cagccccgat
     2881 gatcccgacc ttctgcaaag ccctcctaga gctggttgaa gaacgcccgc tcggcgatgc
     2941 gcttgcgctc ctccgcgctc acctccacgt cgcgtggcgt gagcaggaag accctgttgg
     3001 cgacggactg tatgttgccg tcgagggcgt aggtcaggcc ggagcagaaa tcaaccatcc
     3061 tcctggagag gtccccatcc acattctgga ggttgagtat gacgggttgc tggcgcttga
     3121 agcggtcggc caggttctgc gcgtcgttga agctggctgg ctcgagcacg ctgacgcgcg
     3181 tggtgcgctg gtcaggtacc ggcctcaggt gggccggcgg gttctggttg gcgctgaggc
     3241 ggtccccgcg cgccgacgag tcgctgccga agaggtcccc aagggaagtt ccgaaggccg
     3301 aggagcgctc cgcacgcccg aggcgcctca cggtcggccc cggctcggag cccctggcgc
     3361 cgttcgagta ttcgtcgtca tcgtacctgc ggtccatatc ctcttcgtct tcatagtagt
     3421 catcctcgtc gacaccgaac ccgaaccagg ccgcgatccg gtccagacgg tccctaactc
     3481 ccatcctgcc tccttacggg cccatcctcc tctattagca tccgcccgat ccttaccacg
     3541 gtcgctccct cctcgaccgc gacctcatag tcgccactca tgcccatgga gagttccgag
     3601 agatcgaagt gcgggctcca actttctcgt agcctatcac ggatcgcgcg aagtttcgcg
     3661 aaaac
//
LOCUS       DBNGVD010000110         1701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3120134, whole genome shotgun sequence.
ACCESSION   DBNGVD010000110 DBNGVD010000000
VERSION     DBNGVD010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3120134"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC16CM_03170"
     CDS             <1..864
                     /locus_tag="AC16CM_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3138858.1"
                     /translation="DPHEQSDRDFSRARRRAFLRKVGAYLRGDSGSNQLLSFDEVKDA
                     LGAVSQVYLGMREVPVSQIVGSVGRHRDFDRAFLPSKPDLGTRWRRIDEIMDRGEELP
                     PVSLYKIGDVYFVQDGNHRVSVARQRGVEMLDAEVIELRSRVPLGSALTAHDLLHKLE
                     HRHLLERLPFDRVLPEIKVQFSDVSDYRKLATFIEAHGFRLSQLWRRYVSPEEVLRDW
                     YEYGYRPIAEMIREERILDAFSNRTELDLYLWIVYHRERLALEARDEKISTQTAKDDI
                     MRSAPRRWRPN"
     gene            1076..>1701
                     /gene="sppA"
                     /locus_tag="AC16CM_03175"
     CDS             1076..>1701
                     /gene="sppA"
                     /locus_tag="AC16CM_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3138859.1"
                     /translation="MVGGLVALGLVGLAVFSVVLIAVASGGPTAPALYDEEYVSGDGA
                     DKVAIVPIEGTIASAEDTMGGVLPATSPEGLASALDQAGSDPSVAAVVLEINSPGGGV
                     TASDEMHQSIVDFERNTGKPVVVSMQDVAASGGYYIATAADRIVANETTLTGSLGVIF
                     QITNFAEAADKYGVKQVVIKSGKYKDIGNAFREMTPEERDIFQSIVDES"
BASE COUNT          322 a          550 c          563 g          266 t
ORIGIN      
        1 gaccctcacg aacaatccga cagagatttt tcacgcgccc gcaggagggc cttcctgcgc
       61 aaggtaggcg cgtacctgcg cggggattcc ggttccaacc agttgctctc cttcgacgag
      121 gtcaaggatg ccctgggggc cgtctcgcag gtctacctcg ggatgcggga ggtgccggtc
      181 tcgcagatag taggaagcgt cgggcgtcac agagacttcg accgggcgtt cctgccctcc
      241 aagcccgacc tcgggacccg ctggcgtcgc atagacgaga tcatggacag gggcgaagag
      301 ctgcccccgg tcagcctgta caagatcggg gacgtctact tcgtacagga tggcaaccac
      361 agggtctccg tcgcccgcca gcggggcgta gagatgctgg acgccgaggt aatagagctc
      421 cgcagcaggg ttcccctggg ctcggcgctg acggcccacg acctgctcca caagctggag
      481 catcgtcacc tcctggagcg gctgcccttc gaccgggtac tgccggagat aaaggtccag
      541 ttctccgacg tctcggacta caggaagctc gcgaccttta tcgaggcgca cggcttcagg
      601 ctctcccaac tctggcggcg ctacgtctcg cccgaggagg tcttgaggga ctggtacgag
      661 tacggctacc gccccatagc ggagatgatc cgcgaggagc gcatcctcga cgccttctcg
      721 aaccgcaccg agctcgacct gtacctctgg atcgtctacc accgcgagcg cctcgccctg
      781 gaggcgcgcg acgagaagat ctccacccag accgccaagg acgacatcat gaggagcgct
      841 ccccgccgct ggcgtccgaa ctgaaacggg cggccgggcc ccacctccga aggacgccca
      901 ggtcgagatc cgctctataa tcttcgtggt gaccggagcg ggcatcccgc ccgtcggtcc
      961 tgcaaacgcg cacagcgaag aagggagcgg tatggacact tcccctggcg ggccgcctcg
     1021 tcaacccgag acgcatgcgg aagaaccgag gagacgtcgg cgccgatggc cgttcgtggt
     1081 gggagggctc gtggcgctcg gcctggtcgg gctcgccgtg ttctcggtgg tcctgatcgc
     1141 ggtcgcgtcc ggaggcccga cggccccggc cctctatgac gaggagtacg tctccggtga
     1201 cggggcagac aaggtggcga tagtgcccat cgagggtacg atcgcctccg ccgaggacac
     1261 catgggaggg gtgctgccgg ccacctcccc ggaagggctg gccagcgccc tcgaccaggc
     1321 gggctcagac ccgagcgtcg ccgccgtggt gctggagata aactctcccg gcgggggggt
     1381 cacggcgagc gacgagatgc accagagcat cgtggacttc gagaggaaca cgggcaagcc
     1441 cgtggtggtc tccatgcagg acgtcgcggc ctcgggaggc tactacatcg cgaccgcggc
     1501 cgaccgcata gtcgccaacg aaacgaccct gacgggttcc ctcggcgtga tattccagat
     1561 caccaacttc gccgaggctg ctgacaagta cggcgtcaag caggtggtca tcaagagcgg
     1621 caaatacaag gatataggaa acgccttcag ggagatgacg ccagaagagc gtgacatatt
     1681 ccagtcgatc gtggacgagt c
//
LOCUS       DBNGVD010000111         4457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3485011, whole genome shotgun sequence.
ACCESSION   DBNGVD010000111 DBNGVD010000000
VERSION     DBNGVD010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3485011"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..368
                     /locus_tag="AC16CM_03180"
     CDS             36..368
                     /locus_tag="AC16CM_03180"
                     /inference="COORDINATES: protein motif:HMM:NF042492.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HIR3138860.1"
                     /translation="MAIWNRSPRGGALLLAWMLANPVVFPEPEDDEAWATRAVLGEEM
                     WLAERPMDRAMALNVGATALGLAGAWGAFRRRLLPTAASAVGLVALVLACWREYTLYY
                     ERHRDERG"
     gene            648..893
                     /locus_tag="AC16CM_03185"
     CDS             648..893
                     /locus_tag="AC16CM_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138861.1"
                     /translation="MDAPSQGVEQGGDGERGDHDGELGEPLLSGEGSEEGLDRDYAPE
                     VDQPERYGARAVDEGTVNDHVYVVEALFHDRYPGKDR"
     gene            1939..2151
                     /locus_tag="AC16CM_03190"
     CDS             1939..2151
                     /locus_tag="AC16CM_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138862.1"
                     /translation="MHRAEVLEREIVAHVDGELLTDPDRLAAHLDSAIQRERAALGNP
                     IATVEVWRRRGALPRVCTVVRRSVIA"
     gene            2185..3009
                     /locus_tag="AC16CM_03195"
     CDS             2185..3009
                     /locus_tag="AC16CM_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3138863.1"
                     /translation="MSTIMTPDRVQIFYKDWGEGQPIVFSHGWPLSADDWDTQMMFFA
                     RHGYRVIAHDRRGHGRSEQTGDGHDMDHYADDLAALTAHLDLRDAVHIGHSTGGGEVA
                     HYIARHGESRVARAVLISAVPPLMVQTDANPGGLPKSVFDDLQDQVASNRARLYRDFP
                     SGPFYGFNRPGAEPSEAIIENWWRQGMMGGAKAHYDGVVAFSQTDFTEDLEKITLPVL
                     VMHGDDDQVVPYENSGPRSAELLPNGTLKTYEGFPHGMPTTEAETINADLLEFIRG"
     gene            3191..4147
                     /locus_tag="AC16CM_03200"
     CDS             3191..4147
                     /locus_tag="AC16CM_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138864.1"
                     /translation="MQKELTIPLADEMGVAPLGVGAWAWGTSRFWGYGKEYGRGDVES
                     AFRTSVAENVTLVDTAEIYGNGLSERIIGEILREGGFEKRPVVATKFAPLPYRFGARS
                     LLNAVDKSLERLGIETIDLYQIHFPNPIFKINGLMDALAETVEAGKVRRVGVSNYSAD
                     QMRRAHDRLASHGVPLASNQVHYSLLNRAPETNGVLEACRDLGVTLIAYSPIAQGLLT
                     GKYGPGGNRPAGLVRRVGKAFGEQNLKRVEPVVDILREIGGAHGKEPAQVALNWLITQ
                     KKTLPIPGAKNERQARQNAGALGWEMTHEEAEKLDLATLGWR"
     regulatory      4357..4439
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          871 a         1396 c         1482 g          708 t
ORIGIN      
        1 cctggacccg cctactcagc gcgcccctgt tcttcgtggc gatctggaac cgcagcccga
       61 gaggaggcgc cctcctcctc gcgtggatgc tcgccaaccc ggtcgtgttc cccgaacccg
      121 aggacgacga ggcgtgggcg acgcgggcgg tgcttgggga ggagatgtgg ctcgccgagc
      181 gtcctatgga ccgggccatg gcgctcaacg tcggggcaac ggctttgggg ttggcggggg
      241 cgtggggagc ctttaggcgc cgtctgttgc cgacggcggc aagcgcggta gggctggtgg
      301 cgctggtgct ggcgtgctgg cgcgagtaca ccctctacta cgaacgccac cgcgacgaac
      361 gtgggtgacc ctcttgaact acgcaagggc gaactccgga gaattaacct tctaggacgt
      421 tcggtgaata aggggttgct agtgcgactg cttgcccttt ctttgacgcc gtttcggggc
      481 gcagccacaa ggtagacgtg ggcaggctgc atcgtctccc ttaccacccc ctcaagctct
      541 ctgtcgagtg cctggaggtc cgtctcgtcc ctcagcctcg ccgagaatgc ttccagggtc
      601 ttacgagcgt cgtacttcct cctgtagaag cggcgatcga tgaaggattg gatgcgcctt
      661 ctcagggggt cgaacagggc ggcgatggcg agcgtggaga ccacgacggc gagttggggg
      721 agccgctcct gtccggtgag ggttcggaag agggcctgga tcgtgactat gcccccgaag
      781 tagaccagcc cgagcgttac ggtgcgcgag ccgtagacga gggtacggtt aatgaccacg
      841 tctatgtcgt agaggcgttg tttcacgatc gctatcccgg taaagaccgg tagcccgagc
      901 aggccgaagg cgatgaccgg gtagctgacg tccgtgttcc agatggcgct gaggccaccc
      961 acgaagatgg cgcctacggc caccgcgccc ccgtaggcga gccatttgat ctgctgtcgc
     1021 tccaccgtcc ccgcctggcg catcctgacg acgagcgagg cggcggacgc caccatggag
     1081 actgcaccca ggatgccggc cacggccccc acccacaata ttggactgtc cacaccgtag
     1141 gggttcgtga tctgcgggtg atcttcgaga ggaccgggct ctagcgcgac acccgcgagg
     1201 aagccggtaa tcccgagcac cgcgcaccag gccacgggcc gccagcgcgg tgagggcagc
     1261 cggccgtcgg ggaccaagag caacaggaag gtcgtgggta tgcataccca gaggatccac
     1321 gcccacgagc caaaccaggc cgccgtctcg gcgaggttcc cgggtgccga accgctagcg
     1381 agccggtatt cagcgtagcc tccggtgaag ctgttgaggc ccaccatcgg cccgatgccg
     1441 caaaagatcc aacctatggc gttgcggggc tggcgggagg cgatgacggc gcccaccaca
     1501 gagaaagtga gcagcccgac gaggctgact atcacgttga aaggacccgc cacgctcccg
     1561 tacgtgagct gaagctccgt accgaaagat ctggccacaa gggccaacga gataccgcca
     1621 gcaagcagcg ccacggccag ccccaccagc gaccaggcca gccaggcggc ggtacggggg
     1681 gttatcccac cgattcctcg acgctgatcc tgtgtacctt gagtcacgac accactctgc
     1741 agccgtaccg tctcctgagc gttacggcaa ccgttacggg gccacgcggt agcgagcttc
     1801 ggagaaaaag ccttccacgc tgtgagtatc ctggaggatt gctgtgggcg acgcctcgta
     1861 gcggcctgac cctccttcca cgtgggcccc aagagtgcct tcgaagtgac tcgtcggccg
     1921 caaagcgccc accggaagat gcacagggcg gaggttttgg agcgcgagat cgtggcccac
     1981 gtcgacgggg agctcctgac cgatcccgac cgcctggcgg cgcacctgga ctcggccata
     2041 cagagggaga gggcggccct cgggaacccc atcgcgacgg tagaggtctg gcgaaggcgg
     2101 ggagctctcc cccgggtatg caccgtggta cggcggtctg tgatagcctg agaccgccga
     2161 gaacgagacc tgggaggcaa cgcgatgagc acgatcatga ccccagaccg agttcagatc
     2221 ttctacaagg actggggaga aggccaaccg atcgttttca gccatgggtg gccgctttcg
     2281 gccgacgact gggacaccca gatgatgttc ttcgcgcgcc acggctatcg cgtgatcgcc
     2341 cacgaccggc gcgggcacgg acgctccgag cagaccggcg acggccacga catggaccac
     2401 tacgccgacg acctggcggc gctgaccgcc cacctcgacc tccgggacgc cgtccacata
     2461 ggccactcca ccggcggcgg cgaggttgca cactacatag cccgccacgg cgaaagccgc
     2521 gtggccaggg cggtcttgat cagcgcggtg ccgccgctca tggtacagac cgacgccaat
     2581 cccggcggcc tgcccaagag cgtgttcgat gatctccagg accaggtcgc ctccaaccgg
     2641 gccaggctct accgtgactt tccttctggc cccttctatg gtttcaaccg gcccggtgcg
     2701 gaaccctcgg aggccatcat cgagaactgg tggcgccagg ggatgatggg cggtgcgaag
     2761 gcgcactacg atggggtcgt ggccttctcc cagaccgact tcaccgagga cctcgagaag
     2821 atcacgttgc ctgtcctggt catgcacggc gacgacgacc aggtcgtccc gtacgagaac
     2881 tctggtccgc ggtccgcgga gctcctacca aacgggacgc tgaagaccta cgaagggttc
     2941 cctcacggca tgccgaccac cgaagccgag acgatcaacg cagacctgct ggagttcatc
     3001 cggggctagg tcgtcccgac cggcggaccc tggcaggaga cgaaaagatg gaccacggcc
     3061 gcgaatgctc atctccttct cgcgagagaa gttcgacgct cggacgggcg ggatcatggt
     3121 acaccgatca gagcttacga atgctgcgcc cgagcctgag cagcggggcg aagatatgag
     3181 aggggatgct ttgcaaaagg agctcacgat tcctctggcg gatgagatgg gcgtcgctcc
     3241 tctcggggtt ggtgcttggg cgtggggtac gtcgaggttc tgggggtacg gcaaggagta
     3301 cggccgcggc gacgtggaga gcgccttccg gacgagcgta gccgagaacg tcaccctcgt
     3361 ggacacggcg gagatatacg gcaacggcct ctcggagaga atcatcggcg agatcctgcg
     3421 cgagggcggg ttcgagaaga ggccggtggt tgccaccaag ttcgcccccc ttccataccg
     3481 ctttggcgcg agaagcctgc tcaacgccgt agacaagagc ctggagcggc tcgggataga
     3541 gaccatagac ctctaccaga tccacttccc gaacccgatc ttcaagatca acggcctgat
     3601 ggacgccctc gccgagacgg tagaggcggg caaggtccgc cgggtaggcg tcagcaacta
     3661 cagcgccgac cagatgcggc gggcgcacga ccggctcgcc tcccacgggg taccacttgc
     3721 ctcgaaccag gttcactaca gcctgttgaa tcgtgcccct gagacgaacg gggtgctcga
     3781 agcgtgccgg gacctcggcg tgacgctcat cgcgtacagc cccatagccc agggcctcct
     3841 caccggcaag tacggccctg gcggcaacag gccggcgggc ctcgtgcggc gtgtgggtaa
     3901 agccttcggc gaacagaacc tcaagagggt agagccggtg gtggacatcc tgcgcgagat
     3961 cggcggcgcc cacggcaaag agccggccca ggtggccctc aactggttga tcacgcagaa
     4021 aaagacgttg cctatcccgg gggccaagaa cgaacgccag gcgcgccaga acgcgggtgc
     4081 gttggggtgg gagatgaccc atgaagaagc cgagaagctc gaccttgcga cgctggggtg
     4141 gcgctagacc cgccacccgt agaaagactg cttcgatcta ccgcccggta gccgcccgtc
     4201 gcgcgttctc tgaaacgtag cgctcgaagg ttgcgggctt taccagaacg taacgagtac
     4261 gtttgtcgtg ggtcagaaaa gatccacgag ccgtaatttt gtttacacga ggtgcatttt
     4321 taaggttccc aggatgcaca tgtacggtac aggctttcca cgaaaaggca aacccgtcgt
     4381 gagacggggg cgcaaagcca cgggtctccc ataagaacgg aggtggccgg gctgccgaaa
     4441 ggagaagcaa tgggacg
//
LOCUS       DBNGVD010000112         7084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4031615, whole genome shotgun sequence.
ACCESSION   DBNGVD010000112 DBNGVD010000000
VERSION     DBNGVD010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4031615"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="AC16CM_03205"
     CDS             complement(<1..882)
                     /locus_tag="AC16CM_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138865.1"
                     /translation="MGQKRGIEVENLVRDFKKGPRAVDGIDLRVEPGEIYGFLGPNGA
                     GKSTTVLMLTTLLPPTSGRATVAGYDVVKEGPQVRAAIGAALQEAALDPFLTGREHLH
                     LQGALHGLSRKERRRRGQELLERVGLEEAADRRVRGYSGGMKRRLDLASALVHQPRGL
                     FLDEPTTGLDPVSRKAIWEEVQALNDDGATIFLTTQYLEEADQLADRVGIIAGGLLVA
                     EDTPRALKAEIGNAHLEIAICDGKLERAQEVVAELGKPLPSKDGLLMLELSGGASGVA
                     PVVRALDDAGLAVESLDL"
     gene            complement(882..1751)
                     /locus_tag="AC16CM_03210"
     CDS             complement(882..1751)
                     /locus_tag="AC16CM_03210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3138866.1"
                     /translation="MDNPNGGMGGRTVLVTGGTSGIGKASAVAFAAMGANVVIVGRNP
                     ERGATALRDIKGQSHSDSVELMLADLSVQGEVRRLADQFLERHDRLDVLANNAGLVQS
                     SRTETADGIETTLAINHLAPFLLTNLLLDRLKESAPSRVITVSSEAERMGNIDFDDMK
                     SRHKYKGFAVYGMTKLANIMFTYELAERLKGTFVTANCLHPGAVGTNFGKNNAGPMAL
                     FFRLAKPFMRSPERGADTLIWLASSADVDGVSGKYFSDRKEIEAKEIANDPAARRRLW
                     EISEELTGLKVAV"
     gene            complement(1834..1995)
                     /locus_tag="AC16CM_03215"
     CDS             complement(1834..1995)
                     /locus_tag="AC16CM_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138867.1"
                     /translation="MRHSGETNTFQVRGSGEDNPSVAPRSPRTPDCEEWVWMVDEDPA
                     FYLSETAGA"
     gene            complement(1992..2849)
                     /locus_tag="AC16CM_03220"
     CDS             complement(1992..2849)
                     /locus_tag="AC16CM_03220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3138868.1"
                     /translation="MRSIQVGRVEITALTDMEGAFFGLDQIFPGVAQDQWEPHLRRYP
                     WAFADDDMLWGRVGSYLLRSPEGTVLVDTGAGPQVMGPGGKLLSDLKSAGVEPEDVDT
                     VFLTHLHGDHLGWSVRPDGEPTFANARYVTQAAEWEISVPYLGHAISALDDLGVLELL
                     DGEEPVGEDLTSIPTPGHSPGHSSLLVSSEGQQALVAGDAIVHPAQATEPTWNVQFDM
                     DKEQAARTREMLLAWLEADGIAVAAGHIPGSGFGRVARDAGGRHWQTFDENQEAGLPG
                     IDLSRGGTS"
     gene            2972..3439
                     /locus_tag="AC16CM_03225"
     CDS             2972..3439
                     /locus_tag="AC16CM_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3138869.1"
                     /translation="MDAIERETVIGESCLQLVDEFAAFGPLYLKWVRSRLQDREMTYA
                     RMRLLGTLHCKGPQIMSSISDELGVTRRNVTALVDALEKEGLVRRKPHPTDRRATVIE
                     MTENCELTMDKLYDEHRLAVAGLFADLEEGEREELARMLGVLRRRLQREGICG"
     gene            3520..4479
                     /locus_tag="AC16CM_03230"
     CDS             3520..4479
                     /locus_tag="AC16CM_03230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3138870.1"
                     /translation="MEPDPKPLDVLAIGEMVVDFISDEKTDTLGNASTFRRYLGGSPA
                     NIAVYVSKLGGESAVIAKTGIGAFGTFLKSELQRHGVTTEYLAMDHRTHTTVIFVSST
                     SATPDFEEFRSGDYLLRPDEVSEEAISRTRVVHASTFALSREPCRSAVLWAFSKAREM
                     GKTVSLDPNYSRRVWPDQKEARKVIEEAYCYVDLTKPSADDARRVFGPDLGPEQYIQK
                     FHDLGAQTVVFTMGEGGNLISEYGKTLVHVPARPVEVIDATGAGDAFWAGFLTATLDG
                     HPTERSVLFAREIVERKLRTKGPLPAEIDRAEIYQRLEAGQRG"
     gene            4484..6973
                     /locus_tag="AC16CM_03235"
     CDS             4484..6973
                     /locus_tag="AC16CM_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3138871.1"
                     /translation="MHVAFVNPQGNFDPEDSYWTAHPDFGGQLVYVKELALAMGNLGH
                     KVDIVTRRIIDPDWPEFSSEEDAYPDTPNVRILRIPCGPDTFLPKEELWPYLGTEFVP
                     RLLELYEREGSMPDAVTSHYGDGGLCAALIEERAGIPFSFTAHSLGAQKMDKMGIRRK
                     DMAGVDARYHFSSRIVAERLAMNRSSVNITSTGQERFNQYTHNAYRGAVDPSDDGRFA
                     AVPPGVALHIFDRSARYEDEEAIQKLVDDSLRRDLAAERVGLPCVVASSRLDPKKNHL
                     ALVEAFAGSPTLRESANLVILTGSLENPLEDYGAARDEERAVLDSLMEVVDRAGMRGE
                     VSMFAVRGQRALAAAYRHLAEQRSVFALTALYEPFGLAPLEAMAAGLPAVVTRFGGPS
                     ESMRDGDEEFGILVDPTDPEDVGAGLHALTSSPEAWGRYARAGYGRVLARYTWERTAE
                     GYLEAISGDRDDTAKGNEQGDAPVSADGLRYPGRLTIPSYFTDPSSGEGVSLERLDEL
                     YYGLDLLSVGETMVDFISHEMAQSLRTASQFSRYLGGQPANVAVYVAKLGGRSAVITC
                     LGSDYFGEYVEDQLQHHGVGTEAVSHTDEAATTNAFIARTTSVPDFQVNRGADALLTV
                     RQVDEETISRARIIHTSAFALSRDPQRLAVRRAMRLGSRLGKIVTLDPNYDPRVWPDK
                     VEAWEVLAEVMPYVTIVKPSLEDLRRLFDFNMDEEALEETALAEFHDLGADVVILTRS
                     GGVVTISENGTVERVGPLPKVQVENVTGGRDAFWAAMLMARLDGKHWSEAVRFAHEVA
                     SLKLGVEGHVQRMIDREEIYTRLQTTGDQPT"
BASE COUNT         1252 a         2363 c         2243 g         1226 t
ORIGIN      
        1 cagatcgagc gattccacgg caaggccggc atcgtcgagc gctcgcacga ccggtgcgac
       61 cccgctggca cctccgctga gctcgagcat caggagccca tccttggagg gaaggggctt
      121 gcccagctct gcgacgacct cctgggcgcg ctccagcttg ccgtcgcaga tcgcgatctc
      181 caggtgcgcg tttccgatct cggccttgag tgcccggggc gtgtcctcgg cgacgaggag
      241 cccgccggcg atgatcccca cccggtcggc gagctggtcc gcctcctcga ggtactgcgt
      301 ggtcaaaaag atcgtcgcgc cgtcgtcgtt cagcgcctgg acctcctccc agatcgcctt
      361 gcggctcacg gggtcgaggc cggtggtcgg ctcatcgaga aagaggcccc ggggctgatg
      421 gaccagggcg gaggcgaggt cgaggcgccg cttcatcccg ccggagtagc cgcgcaccct
      481 cctgtcagcg gcctcctcca gcccgacacg ctccagaagc tcctgccccc tccgccggcg
      541 ctccttgcgc gagagcccgt gcagggctcc ctgcaggtgc aggtgctccc ggcccgtcag
      601 gaaggggtcg agggcggcct cctgcaacgc agccccgatg gcagcacgga cctgcgggcc
      661 ctccttgacg acgtcgtagc cggccaccgt agccctgccg ctcgtaggcg ggagcagcgt
      721 ggtcagcatc aggacggtcg tggacttgcc ggcgccgttc gggccgagga agccgtagat
      781 ctcacccggc tcgacgcgca ggtctatgcc gtccacggcg cggggcccct tcttgaaatc
      841 cctgacgagg ttctcgacct ctatgccccg cttctggccc actagacggc caccttcagg
      901 ccggtcaact cctcgctgat ctcccagagc ctgcgccggg cggcgggatc gttggcgatc
      961 tctttggcct ctatctcctt gcggtccgag aagtacttgc cgcttacgcc gtccacgtcg
     1021 gccgaggagg ccagccagat cagggtgtcg gccccccgct cgggtgacct catgaacggc
     1081 ttggctagcc tgaagaaaag cgccatggga ccggcgttgt tcttgccgaa gttggtgccg
     1141 accgcgccgg ggtgcaggca gttggcggtg acgaaggtcc ccttgaggcg ctccgcgagc
     1201 tcgtaggtga acatgatgtt ggccagcttg gtcatcccgt agacggcgaa ccctttgtac
     1261 ttgtgccttg acttcatgtc gtcgaaatcg atgttgccca tgcgctcggc ctcggaggag
     1321 accgtgatca cccggctggg cgcgctctcc ttgagccgat ctagcaacag gttggtgagc
     1381 aggaacggtg caaggtggtt tattgcgagg gtggtctcta tgccgtccgc cgtctcggtg
     1441 cgactgctct ggacgagccc cgcgttgttg gctagcacat ccaacctgtc gtgccgctcc
     1501 aggaactggt ctgccagcct cctgacctcg ccctgcaccg agaggtcagc gagcatcagc
     1561 tccacggagt cgctgtggct ctgccccttg atgtcccgga gggcggtagc cccacgctcc
     1621 gggttgcgac cgacgatcac cacgttcgcg cccatcgccg cgaacgccac cgcggaggct
     1681 ttgccgatgc ccgaggtccc cccggttacg agcacggtcc tgccccccat gccgccgttc
     1741 ggattatcca agatatctcc cgatctagaa aatgcgcgtc ggcccggagt ctatcccgcc
     1801 agaccctccg ggctttgtga gcgccgccac tttctacgcg ccagctgtct ccgacaggta
     1861 gaaagccggg tcctcgtcga ccatccagac ccactcttcg caatccgggg tcctgggtga
     1921 tctgggcgcc acggacgggt tgtcctcgcc gctcccgcgt acctgaaacg tgttggtctc
     1981 gcccgagtgc ctcacgatgt acctcctcta gacaggtcga tgccgggaag gcccgcctcc
     2041 tggttctcgt cgaacgtctg ccagtggcgt ccccccgcgt cgcgcgctac gcggccgaag
     2101 cccgagccgg gaatgtgtcc agccgcgacg gctataccgt ccgcctccag ccacgcgaga
     2161 agcatctcgc gggtgcgggc ggcctgctcc ttgtccatgt cgaactggac gttccaggtg
     2221 ggctcggtag cctgcgcagg gtggacgatg gcatcgccgg ccacgagcgc ctgctgcccc
     2281 tcggaagaga cgagcaggct gctgtgcccc ggcgagtggc cgggggtcgg tatggaggtc
     2341 agatcctccc cgacgggctc ctcgccgtcg agcagctcca gaacgccaag atcgtcgagc
     2401 gcggagatgg catggcccag gtaaggcacg gagatctccc actccgcggc ctgcgtgacg
     2461 tagcgggcgt tggcgaaagt gggctcgcca tctggcctca cactccagcc gagatggtcg
     2521 ccatgcaagt gggtcaggaa gacggtgtcc acgtcttcgg gctccacacc agcgctcttc
     2581 aggtcgctca gcaacttgcc gccgggaccc atcacctgcg ggccggcccc ggtatctacc
     2641 agaaccgtgc cttccggaga gcgcaagagg taagacccga cgcgtcccca gagcatgtca
     2701 tcgtcggcaa aagcccacgg gtatcgtcga aggtgaggct cccactggtc ctgagccacg
     2761 ccggggaaga tctgatccaa cccgaaaaaa gcaccctcca tgtccgtgag cgccgtgatc
     2821 tcaaccctac ctacctgaat gctcctcaag atccaacctc tttccatctt caaaatgatg
     2881 tacatgtaca cattaacatt gacaacaatg aacgtcaaca gtaatatatg gggagaggcg
     2941 ttggtgtgat ggatctctca cgggatgagt cttggatgcg atagagcggg aaacggtgat
     3001 cggagaatcg tgcttgcagc tcgttgacga gttcgcggcc ttcgggcccc tgtacctcaa
     3061 gtgggtcagg tcgcggttgc aggatcggga gatgacgtac gccaggatgc gtttgctcgg
     3121 gaccttgcat tgcaagggcc cccagatcat gagcagcatc agcgacgagc ttggcgttac
     3181 gaggcgcaac gtgacggccc tcgtcgacgc gctcgaaaaa gagggcctgg tcaggcgaaa
     3241 gccccatccc acagaccggc gggctacggt catagagatg acggagaact gcgagctgac
     3301 gatggacaag ctctacgacg agcaccgcct cgcggtcgct gggctgttcg cggacctcga
     3361 agaaggagag cgcgaggagc tagccaggat gctcggcgtc ctgcgaagga ggctccagcg
     3421 tgagggtatc tgcggttaga gcttaatagc tgacagcgcg gttcgcgaag cgaaccgccg
     3481 agggtagacg cggggcgggg aagtttctag actctgttgg tggaacctga tccgaaaccg
     3541 ctcgatgtgc tcgccatcgg ggagatggtc gtggacttta tctccgatga gaagacggac
     3601 acgcttggga atgcttccac cttcaggcgg tatctcgggg ggtcgccggc caacatcgcg
     3661 gtctacgtct ccaagctcgg tggtgaatcg gcggttattg ccaagaccgg gataggcgcg
     3721 ttcgggacgt ttctgaagag cgagttgcag cgtcatggtg ttactacgga gtacctcgcc
     3781 atggaccacc gcacgcatac caccgtgatc ttcgtctcct ccacgtcggc aaccccggat
     3841 ttcgaggagt tcaggagcgg cgactacctg ctgaggcccg acgaggtctc cgaggaggcc
     3901 atctcgcgca caagggtggt acacgcctct accttcgcgc tctcacgcga gccctgtcgt
     3961 tcggcggtgc tctgggcctt cagcaaggcg cgggagatgg gcaagacggt ctccctggac
     4021 cccaactaca gcaggcgggt ctggccagac cagaaagagg ccagaaaggt catagaggag
     4081 gcctactgtt acgtcgacct taccaagcca tccgccgacg acgccaggcg cgtcttcggg
     4141 ccggacctgg gtccggagca gtacatccag aagttccacg accttggcgc ccagaccgtc
     4201 gtcttcacca tgggcgaggg ggggaatctg atctccgagt atggaaagac gctggtccac
     4261 gtccccgccc ggcccgtaga ggtcatcgac gcaaccggtg cgggggacgc gttctgggcc
     4321 gggttcctca ctgccaccct cgacgggcat cccacggagc gctccgtgct cttcgcccgc
     4381 gagatcgtcg aacgcaagct gagaacgaaa gggccccttc ccgcggagat cgatcgcgcg
     4441 gagatctacc agcgcctgga agccggccag agaggatgag cagatgcacg tcgccttcgt
     4501 gaaccctcag gggaacttcg accccgaaga ctcctactgg acggcgcacc cggacttcgg
     4561 cggtcagctc gtctacgtca aggagctcgc gctcgcgatg ggcaacctcg gccacaaggt
     4621 agatatcgtg acccgccgca tcatcgaccc tgactggccc gagttctcct ccgaagaaga
     4681 cgcttacccg gacactccga acgtacggat cctacgcatc ccctgcggac cagacacgtt
     4741 cctgcccaag gaagagttgt ggccctacct cggcaccgag ttcgtaccgc ggctcctcga
     4801 actgtacgag cgtgaagggt cgatgccaga cgccgttacc tcgcattacg gagatggtgg
     4861 cctctgcgcc gccctcatcg aggagcgcgc tggtatccct ttcagcttca ccgcccactc
     4921 actcggcgcg cagaagatgg acaagatggg gattcgacgc aaggacatgg ccggggtgga
     4981 cgcccgctac cacttctcca gccgcatcgt ggccgaacgc ctggcgatga accgctcgag
     5041 cgtgaacatc acgagcacgg gccaggagcg cttcaaccag tacacccaca acgcctacag
     5101 gggggccgtc gacccctcgg acgacggccg cttcgccgcc gttccgcccg gcgtagccct
     5161 gcacatcttc gaccggtcgg cccgttacga ggatgaggaa gccatccaga agctggtcga
     5221 cgattctttg cggcgcgatc tcgccgccga acgggttggc cttccgtgtg tggtcgcctc
     5281 cagcagattg gacccaaaga agaaccacct ggcgctggtg gaggctttcg ccggaagccc
     5341 aaccctacgc gagtcggcca acctggtcat cctgaccggg agcctggaga acccactgga
     5401 ggactacgga gcggccaggg acgaggaacg ggccgtcctc gactcgctga tggaggtggt
     5461 agaccgggct ggaatgcggg gtgaggtctc catgttcgcc gtgcgcggcc agcgggccct
     5521 cgccgccgcc taccgccatc tcgccgagca gcgctccgtc ttcgcgctca cggccctcta
     5581 cgagccgttc gggcttgccc ccctggaggc aatggccgcg gggctccccg ccgtcgtcac
     5641 caggttcggt ggccccagcg agtccatgcg cgacggcgac gaggagttcg gcattctcgt
     5701 ggatcccaca gaccccgagg atgtgggcgc cgggctgcac gccctaacca gcagccccga
     5761 agcctgggga cgctatgcca gggccggcta cggtcgcgtc ctcgcccgct acacgtggga
     5821 gaggaccgcg gaagggtacc tggaggcgat ctccggcgac agggatgaca ctgccaaggg
     5881 aaacgagcag ggcgacgcgc cagtctccgc tgacggcttg cgttatcccg ggcgcctgac
     5941 cataccctcc tatttcacgg acccatcctc gggagaaggg gtttctctgg agaggctcga
     6001 cgagctatac tacgggttgg atctcctctc cgtcggggag accatggtgg actttatctc
     6061 gcacgagatg gcccaatctc tgcggactgc gagccagttc tcacgttacc tcggcgggca
     6121 gccggccaac gtcgccgtgt acgtcgccaa gctagggggg cgctcggccg tgatcacctg
     6181 tctaggctca gactacttcg gcgagtacgt cgaggaccag ctccagcatc acggcgtggg
     6241 caccgaggcc gtgagccaca ccgatgaggc ggcaaccacc aacgccttca tcgcccgaac
     6301 cacgagcgtt cccgacttcc aggtcaacag gggagccgac gcgctgctca ccgtgcgcca
     6361 ggttgacgag gagactatct cgcgcgcccg aatcatccac acctccgcct tcgccctctc
     6421 ccgcgacccc cagcgcctcg ctgtaaggcg ggccatgagg ctgggaagcc gcctcggcaa
     6481 gatcgtcacc ctcgacccaa actacgaccc gcgcgtctgg cccgataagg tcgaggcctg
     6541 ggaggtcctc gcggaggtga tgccgtacgt gaccatcgta aagccctcgc tagaagacct
     6601 acgtcgcctc ttcgacttca acatggacga ggaagccctg gaggagacgg cactcgcgga
     6661 gttccacgac ctcggcgccg acgtggtaat ccttacccgc tccggcggtg tcgtcaccat
     6721 ctcggagaac ggcaccgtgg agcgcgtggg accgttgccg aaggtacagg tcgagaacgt
     6781 aacgggcggc cgcgacgcct tctgggcggc catgctcatg gcccgcctgg acggcaagca
     6841 ctggtccgag gccgtccgct tcgcccatga ggtagcgagc ctcaagctcg gggtcgaagg
     6901 tcacgtccaa cgcatgatcg accgcgagga gatctacacc cgcctgcaga ccaccggcga
     6961 ccaaccgact tgagccgtag aacgttgata cgggtaggag gggccgcctc acccccacgg
     7021 gccccctgtc ctcccgttcg ccccttgcgt tgtccatgct ttcgatcacc tccttgcccg
     7081 ttat
//
LOCUS       DBNGVD010000113         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1663646, whole genome shotgun sequence.
ACCESSION   DBNGVD010000113 DBNGVD010000000
VERSION     DBNGVD010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1663646"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16CM_03240"
     CDS             <1..629
                     /locus_tag="AC16CM_03240"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3138872.1"
                     /translation="ARPRQDAWTTPERPCRSPRVWKELGHDLALLHARVSEDGPAASL
                     ETDKMPDPRPWPNELAEEGYFTATEAGWLEGWLERLAPAALAPVPRCFLHGDSQATNV
                     MVRRGSLEYLAVLDWGSSGWGDPAWDFAGVPLRVVPAMLQGYRAVAPLQSGVEARILW
                     RHLQLSLYLLRRDPQPGYSWAERPLGMLLDVMRFLLDSPGREWSKWAP"
     gene            626..1087
                     /locus_tag="AC16CM_03245"
     CDS             626..1087
                     /locus_tag="AC16CM_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HIR3138873.1"
                     /translation="MKGTIYVAVVGSGEATGNLYERARTVGRLIAERDGIVVCGGLSG
                     VMEAAARGATEAGGVAIGILPDEDRERQNGYLSYSVATGMGQARNLSVVCSGDVVVAI
                     GGEYGTLSEIALARKVGRPVVVLGGWDLGEHVVASSSPEQAVGAAFGFLDG"
     gene            1134..1829
                     /locus_tag="AC16CM_03250"
     CDS             1134..1829
                     /locus_tag="AC16CM_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3138874.1"
                     /translation="MENDRIALVSGGNRGIGLEVCRQLSGRGITVVMGSRDAEQGRAA
                     AAGLPGGVVVHQLDVADPASVEGLARSVEEEYGRLDVLVNNAAISNDEGQSGVDADLD
                     RVKEALEANLFGAWRLCEFAIPMMRRNGYGRIVNVSTGLASLEDMGGGSPGYRVSKTA
                     LNVLTRILASELRGSGILVNAVNPGWVQTDMGGSSATRTVEEGAEALVWAATLPNNGP
                     NGGFFRDREPVAW"
BASE COUNT          356 a          631 c          722 g          364 t
ORIGIN      
        1 gagcgcgtcc acggcaagac gcttggacta ctcccgaaag accctgccga agtccccgcg
       61 tgtggaagga actcggccac gacctcgcac tcctgcatgc ccgcgttagc gaagacggac
      121 cggccgcaag cctggagacc gacaagatgc ccgacccacg cccctggcct aacgagctgg
      181 ccgaggaggg atacttcacg gcaacagagg ctggttggct cgaaggttgg ttggagcggt
      241 tggcccccgc ggcgctcgcg ccagtacccc ggtgtttcct gcacggcgac tctcaggcga
      301 caaacgtgat ggtccgccgg ggatctctgg aatacctggc cgttctcgac tggggaagct
      361 cgggctgggg agatccggcg tgggactttg ccggcgttcc gctcagggtc gtgccagcca
      421 tgctccaggg ctaccgcgcg gttgccccgc tccagagcgg cgtggaggca cgcatcctct
      481 ggcgccatct ccagctctcg ttgtatctgc tgaggcggga tccccaacca ggatactcat
      541 gggccgaacg cccgcttggg atgctcctcg acgtcatgcg tttcctgctc gactccccag
      601 gacgggagtg gtccaagtgg gctccatgaa gggtacgatt tacgtggcgg tcgtcgggtc
      661 tggcgaggca accggcaacc tctatgagag agcgcgtacg gtaggcaggc tcatagccga
      721 aagggacggc attgtcgtct gcggcgggct ctccggcgtg atggaggccg cggcccgcgg
      781 agccaccgag gccggtggcg tggccatcgg catactgcca gacgaggacc gggagaggca
      841 gaacgggtat ctatcttact ccgtggccac gggcatggga caggcgagga acctgtccgt
      901 ggtctgttcg ggggacgtcg tggtagccat cgggggcgag tacgggacgc tctcggagat
      961 agcgcttgcc aggaaggtgg gaaggcccgt cgtggtgctc ggaggttggg acctcgggga
     1021 gcatgttgtg gcaagctctt cgcccgagca agcggtcggg gcagcgttcg ggtttctcga
     1081 cgggtagtat tggttcttct tcggagatgt atatgagaac gggaggtcgc gctgtggaga
     1141 acgatcggat cgcactggta agcggtggaa accgggggat aggcctggag gtctgccgcc
     1201 agctgtcggg gcggggcatc acggtggtca tgggttccag ggacgcggag cagggtcggg
     1261 ccgcagcggc cgggctgccc ggcggggtgg tcgtccacca gctggacgtg gccgaccccg
     1321 ctagcgtcga gggcctcgcg cgctccgttg aggaggaata cggacggctc gacgtgctgg
     1381 tcaacaacgc ggccatctcc aacgatgagg gccagagcgg cgtagacgcc gacctcgacc
     1441 gggtcaagga ggcgctggag gcgaacctgt tcggggcctg gcgcctgtgc gagtttgcca
     1501 tccccatgat gcggcgcaac ggctacgggc gcatcgtcaa cgtctccacg gggctcgcct
     1561 ccctcgagga tatgggcggc ggctccccag ggtaccgcgt ctccaagacc gcgctaaacg
     1621 ttctgacgcg catcctcgcc tccgagttgc gcgggagcgg catcctcgtc aacgccgtca
     1681 accccggctg ggtccagacc gacatgggtg gatccagcgc cacacgcacc gtcgaagaag
     1741 gtgccgaagc cctggtgtgg gccgcaaccc ttcccaacaa cggcccaaac ggcggcttct
     1801 tccgcgaccg cgaaccggta gcctggtgag taggtttcag gcaacaggct accggtatat
     1861 gctggtctgt agagaaatct actctcctcg tatttagccg tcagctctca gccaaaaagc
     1921 tgacggctga ccgctaatag ctcgctctgc aaaggacgag tcgttcggct aacctctcca
     1981 gcttcttcct gaagcctggt acctgttcgc caactagccg ttcttccggg caaagactag
     2041 ccggacgggt gatgtccgtg ttcctggccg ggg
//
LOCUS       DBNGVD010000114         5579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_935434, whole genome shotgun sequence.
ACCESSION   DBNGVD010000114 DBNGVD010000000
VERSION     DBNGVD010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_935434"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC16CM_03255"
     CDS             <1..422
                     /locus_tag="AC16CM_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3138875.1"
                     /translation="TFDKSVSSTAGDFRSLTRLVLMDALKRAGTTVCEPMYRFELDIP
                     ADTFGATSSLLARLGASPRTQELRGQAYTLWGEIPAARVHELEQRLPALTRGEGVLLE
                     SVFDSYRPVRGEIPTRPRTDDNPLDREEYLLRLTRRV"
     gene            434..1336
                     /locus_tag="AC16CM_03260"
     CDS             434..1336
                     /locus_tag="AC16CM_03260"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR3138876.1"
                     /translation="MKAKENGASIDTSLVRRLVDAQFPRWAGLPVEPVGVGGWDNRTF
                     HLGEHMTVRLPSAEGYVAQVEKEHRWLPVLATHLPLPIPVPLAKGVPGEGYPWPWSVY
                     RWIEGETATVGRIRDLVEFATALAEFLVALQNVDPTGGPPPGPHNFFRGGSLEVYDAE
                     TRRALAALDGKIDTRAAREVWESALDAAWHGPPVWLHGDVAWGNLLVQEGRLSAVIDF
                     GTSGVGDPSCDLAISWTLFDGESREAFRAVLQPDDATRARGRGWTLWKALITLAGAPN
                     PGSKDPRPPGRVIDEVLADHEKSA"
     gene            complement(1417..1788)
                     /locus_tag="AC16CM_03265"
     CDS             complement(1417..1788)
                     /locus_tag="AC16CM_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR3138877.1"
                     /translation="MDGTTSSPRITHLSWGRLQTETGTFKDARLFPGGAEEWDWNQTG
                     TSHDPGIQPADVEPLLERGATVIVLSRGFHERLRIAPETHQMLQGKGSPTHIAQTEEA
                     AEIYEDLRTTEKVGALIHSTC"
     gene            complement(1901..2959)
                     /locus_tag="AC16CM_03270"
     CDS             complement(1901..2959)
                     /locus_tag="AC16CM_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3138878.1"
                     /translation="MEGLDLPQGVSSQVVATSRLETHLLASGPEDGIPVLFLHGNASS
                     SRFFGETLAALGPRYRGLAPDLRGFGGSETRPLDATRGLGDFADDVYALVQTLGLESF
                     HLVGWSAGGSAAMQYTMDHPSTVASLTLVDPMSPYGFGGTRDEGGTPCWPDFAGSGGG
                     TANPEFVSRLAANDRTEGDPNSPRNVMNNFYFKPPFRAAQEEAYLSSVLTTQTGEDNY
                     PGDMNLSENWPGVTPGRRGMNNAISPEYCNLEDFALVQPGPPVLWVRGEDDVIVSDTS
                     LLDFGYLGQLDAVPGWPGADVFPPQPMVSQLRAVLDAYEARGGRYREEVIPDCGHTPH
                     VEKPEQFQRLLFGFLEEQ"
     gene            complement(2978..3295)
                     /locus_tag="AC16CM_03275"
     CDS             complement(2978..3295)
                     /locus_tag="AC16CM_03275"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HIR3138879.1"
                     /translation="MYKWGQTNLCSRPTRRADELTREEIRAGTDELSGRLRRFGPRAV
                     AYTGIGVYRWFRATSKVEWGLQPESAVRGVTDVVVPSPSGLNRMRFDELVDHYSALEP
                     LLE"
     gene            complement(3464..3961)
                     /gene="mug"
                     /locus_tag="AC16CM_03280"
     CDS             complement(3464..3961)
                     /gene="mug"
                     /locus_tag="AC16CM_03280"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HIR3138880.1"
                     /translation="MAMDLLGYELKLVFCGYNPSLASGTSGHHYAHPGNRFWRVLLTS
                     GITDRLYEAREDGALLKMGVGFTNLCSRPTRRADELTREEIEAGALALRQKIKRFRPR
                     VVAYTGIGVYKWFLATSKVRWGVQEVSAVPGVTDVVVPSPSGLNRMQLDELVEHYRAL
                     SPYLR"
     gene            4212..4394
                     /locus_tag="AC16CM_03285"
     CDS             4212..4394
                     /locus_tag="AC16CM_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138881.1"
                     /translation="MDNEDTERHQHRDPDIAREERRTLARHHLAVRLGIDPRLAEYLL
                     ELQERIEKLEDRQGSP"
     gene            4486..4830
                     /locus_tag="AC16CM_03290"
     CDS             4486..4830
                     /locus_tag="AC16CM_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138882.1"
                     /translation="MEDDEWMSAQVEEEIEDLRQTHGFNANEATAYWHLQRAAKLMFG
                     MSKTEILEHVAHEEGEVNKELADIIHGAIVGESRVFQHFLALYRELGARVLRRDYPTG
                     WGMSASNEGKDD"
     gene            5159..>5579
                     /locus_tag="AC16CM_03295"
     CDS             5159..>5579
                     /locus_tag="AC16CM_03295"
                     /inference="COORDINATES: protein motif:HMM:NF022019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3138883.1"
                     /translation="MAEPGEDSLSKQEHRSVYLIESVPVGVYKIGYARDPEKRRRELE
                     RRGALGAARCHFGLSWTDKVQPWQSEACKPYRLLWSAPADDYRAAERTLHRLLAEKRI
                     KFESWPKCEWFTLDATDIDYICSIKRFEYGAVIMDKGH"
BASE COUNT         1048 a         1650 c         1887 g          994 t
ORIGIN      
        1 gcaccttcga caagagcgtg tcgagcaccg ccggggactt ccgcagcctg acgcggctgg
       61 tcttgatgga cgcgttgaag cgggccggca ccacggtgtg cgagccgatg taccgcttcg
      121 aactggatat tccagccgac accttcgggg ccacctcgtc cctgctggcc cggctcgggg
      181 cctccccgcg aacgcaggaa ctacggggtc aggcctacac gctgtggggt gagatccccg
      241 cggcgcgggt gcacgagctg gagcaacgac tgccggcgct gaccaggggc gagggcgtgc
      301 tcctcgaatc cgtattcgac agctacaggc cggtccgggg ggagataccc acccgcccgc
      361 gaacagacga caacccgctc gaccgtgagg aatacctgct gcgcctcacg cggcgggtgt
      421 agggagaaac ctcatgaaag cgaaagagaa cggagcaagc atagacacat cgctggtgcg
      481 gcgcctggtc gacgctcagt ttccccgctg ggcgggcctg cccgtcgaac ccgtcggggt
      541 gggcggttgg gacaacagga ccttccacct cggggagcac atgacggtac ggctgcccag
      601 cgcagagggc tacgtggcgc aggtagagaa ggagcaccgc tggttgccgg tgctcgcgac
      661 tcacctgccg cttccaatcc ccgttcccct ggcaaagggg gtcccgggag agggctatcc
      721 ctggccgtgg tccgtgtacc ggtggatcga gggtgagacc gccaccgtgg gacggatcag
      781 ggacctggtg gagttcgcca cggcactggc ggagttcctc gtggccctgc agaacgtaga
      841 ccccaccggt gggccaccgc ctgggccgca caactttttt cgtggcgggt ccctggaggt
      901 ctacgacgcc gaaacccggc gggcactcgc cgcccttgac ggcaagatcg atacccgcgc
      961 ggcgcgtgag gtgtgggagt cggcgctcga cgcggcgtgg cacggaccgc ccgtctggct
     1021 acacggtgac gtcgcctggg gcaacctcct ggtccaggaa ggccggttga gcgcggtcat
     1081 agatttcggg acctcggggg tgggagaccc ttcgtgcgac ctggcgatct cgtggaccct
     1141 cttcgacggg gagagccggg aggccttccg cgcggtgctc cagcccgacg atgccacccg
     1201 ggcgcgggga cgcggctgga cgttgtggaa ggccctgatc acgctcgccg gggcccccaa
     1261 ccctggctcg aaggacccga ggccacccgg gcgggtgatc gacgaagtgc tcgccgatca
     1321 cgagaaaagc gcatgatgaa gaacgcgaac caggaaagct ttcagccatc agcttttagc
     1381 catcagcttt tagccatcag ctttctggtt gctggctcaa caggtcgagt ggatcagggc
     1441 tcccaccttc tcggtggtcc gtagatcttc gtagatctcg gctgcctctt cggtctgggc
     1501 tatatgggtt gggctgcctt tgccctggag catctggtgt gtctccggcg ctatgcggag
     1561 gcgttcgtgg aagcctctag agaggacgat aacggtggcg ccacgctcta gcagcggctc
     1621 cacgtcggcg ggttggatgc cggggtcgtg gctggtgccg gtctggttcc agtcccactc
     1681 ctcggcgcct cccgggaaga gcctggcatc cttgaaggtg ccggtttccg tctgcaggcg
     1741 gccccaggag aggtgggtta tgcgtggcga ggacgtggtc ccgtccatgt cggctatccg
     1801 ctggagcggc gtctagttga cactgaagcg gaggagaaga aaggcaacgg gcatctggta
     1861 tttttcctga agcctgaaac ccgtagcctg aagccctgtc tcactgttct tccaggaagc
     1921 cgaatagtag ccgctggaac tgctccggtt tttcgacgtg cggggtgtgt ccgcagtcgg
     1981 ggatgacttc ctcgcggtag cgaccgccgc gggcctcgta ggcgtcgagc acggcgcgga
     2041 gctgggagac catgggttgg ggaggaaata catcggcgcc gggccaacca gggacggcat
     2101 cgagctggcc gaggtagccg aagtctagga gggaggtatc ggagacgatc acgtcgtcct
     2161 cgcctcttac ccagaggacg ggcggccctg gctgcaccag ggcgaaatct tccaggttgc
     2221 agtactccgg cgagatggcg ttgttcatgc ccctcctgcc gggagtcacg ccgggccagt
     2281 tctccgagag gttcatgtcg cccgggtagt tgtcttcgcc cgtctgcgtc gtgagcaccg
     2341 aggagaggta ggcctcctcc tgcgccgccc tgaagggcgg tttgaagtag aagttgttca
     2401 tgacgttgcg gggcgagttg gggtcacctt ccgtcctatc attggccgcc agccgggaca
     2461 cgaactccgg gttggccgtg ccgccaccgg agcctgcgaa gtcaggccag cagggtgtgc
     2521 cgccctcgtc cctggtaccg ccgaagccgt agggcgacat gggatcgacg agggtgagcg
     2581 aggcgacggt ggagggatgg tccatcgtgt actgcatggc cgcgctacca cccgcggacc
     2641 aacccacgag gtggaagctc tccaggccga gtgtctgcac gagggcatac acgtcgtctg
     2701 cgaagtcccc gaggccccgc gtcgcatcta gcggtctcgt ctcggagcca ccgaaaccgc
     2761 gcaggtcggg ggccaggccg cggtagcgcg ggccaagcgc cgcgagcgtc tccccgaaga
     2821 agcgcgagga ggaggcgttg ccgtgcagga agaggacagg gatgccgtcc tcgggcccgc
     2881 tcgcgagcag gtgggtctcg agccgggatg tggcgacgac ctgtgagctt acgccttgcg
     2941 ggagatccag accttccatc tcatgcctcc tctgtggtta ctctagcagg ggttcgaggg
     3001 cgctgtagtg gtcaacgagc tcatcgaagc gcatcctgtt caggcctgac ggagacggga
     3061 ccacaacgtc ggtcacgccc cgcacggcgg attcgggctg gaggccccat tcgaccttcg
     3121 aggtagcccg gaaccatctg tagacgccta tgcccgtgta ggcgacggct cggggaccga
     3181 agcgcctgag cctgccgctc agctcgtcgg ttccggccct gatctcctcc ctggtgagct
     3241 cgtccgcacg gcgcgtcggg cgggagcaga ggttggtctg accccattta tacaaacaac
     3301 cccctaacct tacacgctca cctcggttct gacggtgaga ttagcatagt cgtaccctcg
     3361 attgcgctcc accagacccg cgaggatcgc gtgtaaggcc cacagtcgcg ctgtggggcg
     3421 tagtcacgac tcgtccgcct atagaggtga catgagttcg accttacctc aagtacggcg
     3481 aaagcgcgcg gtagtgctcg acgagttcat ctaactgcat gcggttgagc cccgagggtg
     3541 atggcacgac cacgtcggtc acccccggta cggccgatac ctcttgcacg ccccacctca
     3601 ccttcgaagt cgccagaaac catttgtaga cgcctatgcc ggtgtaggca acgactcggg
     3661 gtcggaaccg ctttatcttc tggcgtaagg cgagagcgcc tgcctcgatc tcttcgcgcg
     3721 tcaactcatc ggcccttctg gtgggtctgc tgcaaagatt ggtaaatccc acgcccattt
     3781 tgaggagcgc cccatcctcc cgagcctcat atagcctatc ggtgatgcca gaagtaagaa
     3841 ggacgcgcca gaagcgattg cccggatggg cgtagtgatg accgctggtg cccgatgcga
     3901 gtgagggatt atagccgcag aaaacgagct tcagttcgta accgaggaga tccatggcca
     3961 taaggacagt ctaacggcac agcggttcca ttacctcgct acagtctgtt ttcttcccat
     4021 gctctcctgg atcggttacc gcgcccgcct gacccccgac ggcgaggcca cgctcgcagt
     4081 tagcgtgtga ggccgccagg ggcgggagga tcgcccacag aggtcgagca cgcaaacgta
     4141 taatggtgtg gagtgcgcac ggaacccgtg atggggtagg gtgttcgggt gtaggaagta
     4201 aggaggtcgc tgtggataac gaagacaccg agagacacca gcaccgtgat ccggacatcg
     4261 ccagagaaga gagacgaacc ttggcaagac accacctggc ggtaagactg ggaatagacc
     4321 ccaggctcgc ggagtaccta ctcgaactcc aagagaggat agagaagcta gaggataggc
     4381 agggttctcc gtagagcccc agtccgatgc tccaggcccg caggagggcg tacgtcgccc
     4441 gtggtggcgt aggttgttcc gaggtacgat cgagaggaac gtggtgtgga agacgacgaa
     4501 tggatgagcg cgcaagtcga agaggagatc gaggacctgc gccaaactca cgggttcaac
     4561 gcaaatgagg ccaccgccta ctggcacctc caaagggccg cgaagctgat gttcgggatg
     4621 agcaagacgg agatcttgga gcacgtcgcc cacgaggagg gcgaggtcaa taaggagctc
     4681 gcggacataa tccacggagc gatcgtaggc gagtcgaggg tcttccagca cttcctggcc
     4741 ctctataggg aactaggcgc aagggtgttg cgcagggact acccgaccgg atggggtatg
     4801 agcgcctcca acgaaggcaa agacgactaa cctttcgaaa cgcgctcgga gaaccgctca
     4861 cggttgcaag gatcttttgt tccgagagga gcataacctc gggccagagc agtctccaca
     4921 ggacgagcca cggcgcggat tctggcttcg gctggtgtag gtaccgtact acgaactaga
     4981 gatcggtttc cgcgcccgcc tgacttccga cggcggggcc acggtcgaag tgcgcgtgtg
     5041 atgcctgtca gaggtgcgat ggccgcacac aggggccgcc gagtcatcag ggacgtataa
     5101 tcttctgata cagcgatttg ggagagtgaa gcgaggtcct ggcggaggag gtcgttcggt
     5161 ggctgaaccg ggagaagatt cgctctcgaa gcaggaacat cgctccgtct acttgattga
     5221 gtccgtaccg gtcggcgtct acaagatcgg gtacgcccgc gaccccgaga agcgtaggag
     5281 ggagttggag cgtagaggag cgctcggggc ggctcgctgt catttcggct tgtcgtggac
     5341 cgacaaagtt cagccgtggc agtcggaggc gtgtaagccc taccgtttgc tgtggagcgc
     5401 ccctgcagac gactaccgcg cggcggagcg cacgttgcac aggttgctcg ctgagaagcg
     5461 catcaagttc gagagctggc cgaaatgcga gtggtttacc cttgacgcta ctgatatcga
     5521 ctacatctgc tccattaagc gcttcgaata cggggctgtg attatggaca aggggcatg
//
LOCUS       DBNGVD010000115         9220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1664080, whole genome shotgun sequence.
ACCESSION   DBNGVD010000115 DBNGVD010000000
VERSION     DBNGVD010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1664080"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="murJ"
                     /locus_tag="AC16CM_03300"
     CDS             complement(<1..1296)
                     /gene="murJ"
                     /locus_tag="AC16CM_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3138884.1"
                     /translation="MTGILRSMLSISVATVLSRVTGYGRWMAQAAVLATGLVADAYTA
                     SILLPSLIYELFMGGILYSIFIPVLVDRITKHGEEDARLLTNALFTLVLPLMLVLSLI
                     GIVFAEPLVGLVTNFQAAESLSPREIEEVQGLAAFFFRIFVLQMFFYGISTIGTGVLQ
                     AHRRFFLPTFAPVLNNLLVIASFVAYHFLRQSDERLALYTLAFGVTVGVAVMALALVP
                     TMLALGYRPRLQFGHPALIPTARLAGPMVVLVAASVGFQLFGAYLATGFRGGVASMNY
                     AFTIFSLPYGVFVVAVTTALMPELSEKYSRSDTDGYRDTFSFGLRTMVFVVVPSVVGL
                     ISLSQPIVGLLYERGNFDATDTRIVAGLLVIYAAGLLGYSVYFYLVRAFYSRQNTRTP
                     ALLNVVIFLLYAALAFGLSRIWGIAGVVLGLAVAYSVLAV"
     gene            complement(1670..2515)
                     /locus_tag="AC16CM_03305"
     CDS             complement(1670..2515)
                     /locus_tag="AC16CM_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3138885.1"
                     /translation="MKGRSKNPLPPALVLAFALSIALVLLVGCDAGTEDSYQGSSQME
                     ETTGLSGGVQGKITVASNIAYPPFEFSPRGKPKGFDIDLMNEIARRAGFEVRYQNVQF
                     DSILRGLDAGLFDASISAMSITPEREKQLDYSDPYFNADQALLVAGDSKIKAIDDLTE
                     ATVGVQAGSTGQLKGEDLYDDGQIGEIKQYMTIGEALSALKAGKIDGVIYDISAVNKK
                     VTENKGEIRYVEPISTGEQYGIAFPEDSVLVEPVNQALAEMKEDGTYEKLYKKWIGIP
                     PEEIP"
     gene            2772..4052
                     /locus_tag="AC16CM_03310"
     CDS             2772..4052
                     /locus_tag="AC16CM_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIR3138886.1"
                     /translation="MAKPNGDLARAVGEAVWARRERAVALLQELVNVRSVTGGEGAVA
                     GVVERAFSERGLEVDTWNASPGEMEPYIEQVGEQPSYENRPNIAGLMRGSGGGRSILL
                     NAHTDTVDPGDPAAWTHDPLSGAVDGDLLYGRGSCDMKGGLVTYLTAIEALSDLGLDL
                     RGDVTVAATVGEENGGLGALSTVLRGYRADAALITEPTRLALVPAQGGSLVFRLKISG
                     RSAHAATRDEGVSALEKFVPIFQDLQELERERNAALSHPLYDGIENKVPINVGLVRAG
                     NWASTVPESLVAEGRVGLIPGEEVDSFKDLVAERIETVAKRDPWLREHPPELQWFGGQ
                     FAPAEIEPDAPICEALKRAHEKVTGEKPPVEGVPYGADMRLFIHFGGMPCVMYGAGDV
                     RVAHAPDEHISITDLLTATSTIACLLADWCGGGQ"
     gene            4144..5580
                     /locus_tag="AC16CM_03315"
     CDS             4144..5580
                     /locus_tag="AC16CM_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3138887.1"
                     /translation="MESSGSKSAADRNVAGQSEGLVRAISAPLLFFYVLGDVLGSGIY
                     ALIGVMAAQVGGAFWTSFVIGVVAAMLTGFAYAELITKYPHAAGAAMYAGRAFENRFF
                     TFLVAFCMVAASLAAAGALALAFGGYFLELLSELLSLPLLLTALVFILVLAYINFRGI
                     SESVKANLAMTITEVVGLALVLVVGVVVLLSGEANFSRPFEFNDGSNPALLALTGATL
                     AFFAMTGFENAANVAEEVQNPSRVYPLALLGGMASAGVIYLVIAFIASMVTPLGQLAG
                     SDVALLEVVRQGMPAFPGWLFAIIACVAVTNTCLVSLITNSRIMYGMAREDVVPRIFA
                     RTHQSRRTPWVAIVFTTIIALILIVGVGETGVNTLASATVAFLLAVFALVCLCALVLR
                     RDSVSQDHYTAPTAILGLGVVVNVALLLYVLITDIQGLVAGDVGLRESVSVVCLVMLV
                     VGAGLYFANNLAQRRLDPSRTDDRNGRG"
     gene            5595..6047
                     /locus_tag="AC16CM_03320"
     CDS             5595..6047
                     /locus_tag="AC16CM_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3138888.1"
                     /translation="MTHVLIATDGSEQSLKAARYLRSLLDPASLERVSVIAVVRPLAA
                     VPFASDFGEEEHAAQEAEDAGGYSFQRAAQEAVERVAEELRGLTTNVDTIVRGGAPAD
                     QIIRAADDLEADLIVVGGRGKGAVEAIVLGSVAYRVLHHAPCPVLVTR"
     gene            6201..6851
                     /locus_tag="AC16CM_03325"
     CDS             6201..6851
                     /locus_tag="AC16CM_03325"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3138889.1"
                     /translation="MDSLFDVAEGGEDREKRLEELARQVSVCTKCDLAKTRTNTVFAS
                     GDPYSPLMLLGEGPGENEDATGLPFVGRAGKLLDDILAAVDLTRQQVYLTNVVKCRAA
                     VEEGGRMKNRQPKTGEIRACDPYLRGQIEAVRPEIMLCLGGPAAKAMIDKDFRITKDR
                     GRWHDIDGIKTMATFHPAYVLRQRGDDLTNTKRLVWKDIQNVYAEYQKAMEARESS"
     gene            7058..8092
                     /gene="ahbA"
                     /locus_tag="AC16CM_03330"
     CDS             7058..8092
                     /gene="ahbA"
                     /locus_tag="AC16CM_03330"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit alpha"
                     /protein_id="HIR3138890.1"
                     /translation="MLDSTDREILNRIQREVPLEREPFEAIGRELGIAGDEVIRRIEA
                     LKRGRVIRQISAIFDTRVLGYESSLVAATIPARKLNEGAKAINSHPGVSHNYERNNEF
                     NLWYTVAVPPDSRLGLQGTVDVLHHISGAEKTRVLPTLKLFKIGVTLDMNKGATAKKE
                     APQYGEVDRQGADRNISEDDKAAIKALQEDVPLTPRPFDLWGRQVGLSYEELLERAYD
                     LRDRKIMRRFSAVLYHRKAGFRANAMGVWRVPDERIDEVGDAFAHYQAVSHCYQRPTY
                     EDWPYSVFSMVHGRSVEECENVLAAMAEETGLTDYTSLYSTREYKKTRVRYFTPEMEA
                     WERLYAGILR"
     gene            8158..8898
                     /locus_tag="AC16CM_03335"
     CDS             8158..8898
                     /locus_tag="AC16CM_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3138891.1"
                     /translation="MLSAALIVALFAALLAGIVAGLTGFGLALISTPILLFVYEPRTV
                     VVLTVIFSVFITAAVVWDSWREAHRRLALGLLVPALFGVVVGTVVLDAVDPNYIRLAV
                     GAIVVFSALLLVRDFRLPGANTGWGTLVAGSASGALTTSTGLAAPPIVLLLASRGLPK
                     DEFRGTTALYFLPMSIAGFAVLAARGLVEAPEIPLGLALVPAAIIGKAIGTALLKRTS
                     ENAFQNITLGLVILTGTLGVLTATWALL"
     gene            complement(8948..>9220)
                     /locus_tag="AC16CM_03340"
     CDS             complement(8948..>9220)
                     /locus_tag="AC16CM_03340"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3138892.1"
                     /translation="APDLFVNLVASTREGDLETAARLHRRVLELVTLGVHSDPPIGAI
                     KLAMRMLGVPISPAVRGPALPADEGAEEQIAAILENAGVLDPSKSS"
BASE COUNT         1614 a         2917 c         3082 g         1607 t
ORIGIN      
        1 gacggccagc accgagtagg ctaccgcgag cccgagcacg acgccggcga tgccccagat
       61 ccgggagagc ccgaaggcga gggcggcgta gagcaggaag atcaccacgt tcagcaaggc
      121 tggggtcctg gtgttctgcc gcgagtagaa ggcgcgcacc aggtagaagt aaaccgagta
      181 tccgagcagc cccgccgcgt agatcacgag cagcccggcc acgatcctgg tgtccgtggc
      241 atcgaagttt ccgcgctcgt agagcaaccc gacgatgggc tgggagagtg agatcaggcc
      301 gaccaccgac ggtacgacga cgaagaccat ggtcctcaag ccgaaggaga aggtgtcgcg
      361 gtagccgtcg gtatcgctgc gagaatattt ctcggagagc tcgggcatga gcgcggtcgt
      421 gacggcgacg acgaacacgc cgtagggtaa agagaagatc gtgaaggcgt agttcatgga
      481 ggccacccct cccctgaacc cggtggcgag gtaggcgccg aacagttgaa aaccaaccga
      541 ggcggcgacc agaacgacca tggggccggc gaggcgcgcc gtcggtatga gggccgggtg
      601 gccgaactgt aggcgaggtc tgtaaccgag tgccaacatc gtgggcacca gcgcgagggc
      661 catgacagcc acgcccaccg tcacgccgaa ggcgagcgta tagagcgcca gccgctcgtc
      721 gctctgccgc aagaagtggt aggccacgaa cgaggcgatg accagcaggt tgttcagcac
      781 gggggcaaag gtgggaagaa agaagcgcct gtgggcctga aggacgccgg tgccgatggt
      841 gcttatcccg tagaagaaca tctgcaggac aaagatccgg aaaaagaacg ccgcgagccc
      901 ctgaacctcc tcgatctcac gcggggacag gctctccgcc gcctgaaagt tggtgaccag
      961 ccccacgagc ggttcggcga acacgatgcc gatcagcgag agaacgagca tcagcggcag
     1021 caccagcgtg aagagggcgt tcgtcaggag gcgggcgtcc tcttcgccat gcttcgtgat
     1081 tcggtcaacc agcacgggga taaagatcga atacagtatc ccgcccatga acagctcgta
     1141 gatcaggctc ggcaacagga tagaggccgt gtaggcatcg gccaccaacc ccgtagcgag
     1201 gacggcggcc tgcgccatcc acctcccgta ccccgtaacc ctcgacagaa cggtggccac
     1261 ggagatcgat agcatcgacc tgagaatccc ggtcatacga aggcttcgcc ttggcagcac
     1321 agcgcctgtc ggcgccttgt cagcccactc gggctggcgc cctcgcttgt cagctcgctc
     1381 cagcctgaag gctgtcgctt gtcagcacgc ttcggcccct gcggggcttc gctttcagga
     1441 agtcgtctcg ctccgtaact gcaagccagg catgagattc ctctacgcac cgatacatcc
     1501 cgtgcgtaag gtgcaggccg taacgggatc ctgctgaaag cgagccaggc ggagccgggc
     1561 gaagcgttgc cgacgagcga gggcgtaagc ccgagcgtgc tgacaagccg gcgtagcgaa
     1621 gcgaagctgg cgtgctgacg agcggaggcc cgaagggccg cagcgtgctc tatggtatct
     1681 cttcgggggg gatcccgatc catttcttgt agagtttttc gtaggtgccg tcttccttca
     1741 tctcggccag cgcctggttt acgggctcga ccagcacgct gtcttccgga aaggcaatac
     1801 cgtattgctc gccggtcgag atgggctcga cgtacctgat ctcccctttg ttctccgtca
     1861 ccttcttgtt tacggcagag atgtcgtaga tgacgccgtc gatcttgccg gctttgaggg
     1921 ccgagagggc ttccccaata gtcatgtact gcttgatctc gccgatctgc ccgtcgtcgt
     1981 ataggtcctc gcccttgagc tggcccgtgg agccggcctg gacgccgacg gtggcctcgg
     2041 tgaggtcgtc gatggctttg atcttcgagt cgcctgcgac caggagcgcc tggtcggcgt
     2101 tgaagtaggg gtcagaatag tcgagctgtt tctcgcgctc tggggttatg ctcatggccg
     2161 agatggaggc atcgaacagg ccggcatcca ggccgcgcag gatgctgtcg aactgcacgt
     2221 tctggtagcg gacctcgaag ccggccctcc tggcgatctc gttcatcagg tcgatgtcga
     2281 accctttggg cttgcccctg ggtgagaact cgaagggcgg gtaggcgatg ttggaggcga
     2341 ccgtgatctt gccctgcacg ccccccgaca ggcccgtggt ctcttccatc tgcgagcttc
     2401 cctggtagga gtcctcggtt cccgcgtcgc agccaacgag gagaaccagc gcgatagaca
     2461 gggcaaaggc gaggacgagc gccgggggga ggggattctt cgatcgtcct ttcacctgga
     2521 aactatccct ccgtgttcgt gctctctggt ctaacccggc cgtgacagaa agggcggtca
     2581 cggacggacc cggccgccgg caagctggcc ttcgcgccgt cttttcgcgc gcggggtaag
     2641 tacatcatat agtcccgccc caaaaggatg attctaatta cacggcggcg tcaattcaag
     2701 ctcggcggac attgccgtgg gaccctgccc gtgctacggt gccgcggcgt tgggaaccgg
     2761 agggaggaat cgtggcgaag ccgaacggag atctggcacg ggccgtgggc gaggccgtgt
     2821 gggcgaggcg cgagagggcc gtggccctgc tgcaggagct tgtgaacgtc cgctcggtga
     2881 cgggcgggga gggcgcggtg gccggggtgg tcgagagggc tttctcggag cgtggcctcg
     2941 aggtcgacac ctggaacgcg agccccgggg agatggaacc ctatatcgag caggtcgggg
     3001 agcagccctc ctacgagaac cgccccaaca tcgccggcct catgaggggg agcgggggcg
     3061 ggcgttccat cctcttgaac gcccacaccg acaccgtcga ccccggagac cccgcggcgt
     3121 ggacgcacga tccgctctcc ggcgccgtcg atggcgacct gctctacgga cgtggctcct
     3181 gcgatatgaa gggcggactc gtcacctacc tcacggccat cgaggccctc tctgatctcg
     3241 gcctggacct gcggggcgac gtcaccgtcg ctgccacggt gggggaggag aacgggggcc
     3301 tcggcgccct ctctaccgtt ttgcgcggat accgcgccga cgccgccctc ataaccgagc
     3361 cgacccgcct cgccctcgtg cccgcccagg ggggctcgct tgtcttcagg ctcaagatct
     3421 ccggtcgttc ggcccacgcc gccacccgcg atgagggcgt ctcggccctg gagaagttcg
     3481 tgcccatctt ccaggacctg caggagctgg agcgtgagcg caacgccgcc ctcagccatc
     3541 cgctctacga tgggatagag aacaaggtgc cgatcaacgt gggcctcgtc agggccggca
     3601 actgggcctc caccgtcccc gagtcgctcg tcgccgaggg gagggtcggt ctgatcccag
     3661 gcgaagaagt ggattctttc aaggacctcg tggccgaacg catcgagacg gtggcgaagc
     3721 gggatccctg gctccgcgag catcccccgg agctccagtg gttcggtggc cagttcgccc
     3781 ccgccgagat cgagcctgac gcccccatct gcgaagcctt gaagagggcc cacgaaaagg
     3841 tgaccgggga gaagccgccc gtcgaaggcg tcccctacgg cgcggacatg cgtctcttta
     3901 tccactttgg tgggatgccc tgcgtcatgt acggcgctgg cgatgtgagg gtggcccacg
     3961 ccccagacga gcacatcagc atcaccgacc tcctgaccgc gaccagcacc atcgcctgcc
     4021 tcctcgccga ctggtgcggc gggggccagt agttgcatcc gttcgtgatg ttgccccggg
     4081 gtcggcccat cggtacactt cgtggccgta gccagcgtag catgacagaa ggataggaga
     4141 actttggaga gttcgggctc gaagtcggcc gccgaccgta acgtggcagg gcaatccgag
     4201 ggtctggtgc gggccataag cgcgccgctt ctgttcttct acgtgcttgg ggacgtactg
     4261 ggatccggca tctacgccct gatcggggtg atggcagccc aggttggggg ggcgttctgg
     4321 acctcgttcg taatcggggt ggttgccgcc atgctgaccg gcttcgccta cgccgagttg
     4381 atcaccaagt acccccacgc ggctggcgcc gccatgtacg ctggccgggc cttcgaaaac
     4441 cgcttcttca cctttctggt cgcgttctgc atggtggccg cgagcctggc agccgctggt
     4501 gccctggcgc tggcgtttgg cggatacttt ctggagcttc tttcggagct tctgagcctg
     4561 ccgctactcc tgacggccct cgtcttcatc ctggtgctcg cgtacatcaa cttccgcggc
     4621 atctcggagt ccgtaaaggc gaacctcgcc atgaccatca ccgaggtggt cggcctggcg
     4681 ctggtgctcg tcgtcggggt cgtcgtgctg ctgagcggtg aggcgaactt cagccggccg
     4741 ttcgagttca acgatgggag caaccccgcg ctactggccc tgaccggcgc cacgctcgcg
     4801 ttcttcgcga tgaccggctt cgagaacgcg gcgaacgtgg ccgaggaggt tcagaacccg
     4861 agcagggtct acccgcttgc gctgcttggc ggcatggcct ctgctggggt catctacctg
     4921 gtcatcgctt tcatcgcctc gatggtgacg cccctcggcc agctcgcggg gtcggatgtg
     4981 gcgctgctcg aggtggtgcg gcagggcatg ccggccttcc cgggttggct gttcgcgatc
     5041 attgcgtgcg tggcggtaac gaacacttgc ctcgtctccc tgataaccaa ctcacggatc
     5101 atgtacggca tggcacgcga ggacgtggtc ccgcgcatct tcgcccgcac ccaccaatcg
     5161 cggcgcacgc cctgggttgc catcgtcttt accaccatca tcgcgctgat cctgatcgtt
     5221 ggcgtcggtg agacgggcgt caacacgttg gccagcgcca ccgtggcgtt ccttctggcg
     5281 gttttcgcgc tggtctgcct gtgcgcgctg gtactgcgcc gcgacagcgt gtcccaggac
     5341 cattacacgg cgccgacggc gatcctgggc ctcggggtgg tggtgaacgt ggcgcttctg
     5401 ctctacgtcc tgataaccga cattcagggc ctcgtcgccg gggacgtcgg gttgagggag
     5461 agtgttagcg tggtgtgcct ggtgatgctg gtggtagggg ccgggttgta cttcgcgaac
     5521 aacctcgcgc agcgcaggct ggacccatcg cggaccgacg accggaacgg ccgagggtag
     5581 acaggaggag gaccatgacc cacgttctga tcgccaccga cggttcggag cagtcgctca
     5641 aggcagcgag ataccttcgc tcgctcctgg atcccgcctc gctggagcgg gtcagcgtca
     5701 tagccgtggt ccggcccctg gccgccgtgc cgttcgcgag cgacttcggc gaggaagagc
     5761 acgccgccca ggaggccgaa gatgcaggtg ggtactcgtt tcagcgggcg gcgcaggagg
     5821 ccgtagagcg cgtcgctgag gagctccgag gcttaaccac gaacgttgac accatcgtcc
     5881 gcggcggcgc gcccgccgac cagatcatcc gcgccgcgga tgacctcgaa gccgacctga
     5941 tcgtcgtcgg cggccggggg aagggggcgg tcgaagccat cgtgctcggc agcgtggcct
     6001 acagggtgct gcaccacgcg ccctgccccg tgctcgtaac ccggtaggga gcgggaggcc
     6061 gttccgcatc ccctggaggc ggggcgcgtc gagacggcgt tcgcggatcg cgccgccggc
     6121 ccgtagaacc ctcgcgggac gttcccccag ggtgtgcggt gtagaatagc ccgcaggcaa
     6181 gctagcaatc tggaggagcc ttggacagcc ttttcgacgt cgcggagggc ggcgaggacc
     6241 gcgagaagcg gctggaggaa ctcgcccgcc aggtctccgt gtgcaccaaa tgcgacctgg
     6301 cgaagacccg caccaacacg gtgttcgcct cgggggatcc ctactcgccc ctcatgctcc
     6361 tcggcgaggg gcctggcgag aacgaggacg ccacggggtt gcccttcgtg ggtagagccg
     6421 gcaagctgct cgacgacatc ctggcggccg tggacctgac gcggcagcag gtctacctga
     6481 ccaacgtcgt caagtgccgc gccgccgtcg aggagggcgg caggatgaag aaccgccagc
     6541 cgaagacggg ggagatcagg gcctgcgacc cctacctgcg gggccagatc gaggcggtca
     6601 ggccggagat aatgctctgc ctcgggggtc ccgccgcgaa ggccatgata gacaaggact
     6661 tccgcatcac caaagatcgc ggccggtggc acgacataga cggcataaag acgatggcaa
     6721 ccttccaccc cgcctacgtg ctgaggcaac gcggcgacga cctgaccaac acgaagcgcc
     6781 tggtctggaa ggacatccag aacgtctacg ccgagtacca gaaggcgatg gaagcgcggg
     6841 aatcctcgta gcttgcggag gtccttcgca agctataagc tatcggttct tcggcaagaa
     6901 caagaagctg accgctgttg ctgacggctg aaagctcaca acaacgtaaa cattctcata
     6961 ccgagtttcg gtctcgcctg ttatagtgcg tttatgaagt agagaaaccg tcgagaaggg
     7021 ctcgtaaagc gtggtaagca caactaccgg cagtcgcatg ttggacagca ccgacaggga
     7081 gatactcaac cgcatccagc gcgaggtccc gctggagcgg gaacctttcg aggctatcgg
     7141 gcgtgagctt ggtatagcgg gggacgaggt gatccggcgc atagaggcgc tcaagagagg
     7201 gcgggtgata cgccagatca gcgcgatctt cgacacgcgc gtccttggct acgagtcgag
     7261 cctggtcgcg gccaccatcc cggcccgcaa gctgaacgag ggcgcgaagg cgatcaactc
     7321 ccaccccggc gtctcccaca actacgagcg caacaacgag ttcaacctgt ggtacacggt
     7381 cgccgttccg cccgactccc gactggggct gcagggcacc gtggacgtgc ttcaccacat
     7441 tagcggcgcg gagaagaccc gcgtcctgcc aaccctgaag ctgttcaaga tcggggtgac
     7501 cctggacatg aacaagggcg ccacggccaa gaaagaggcg ccccagtacg gcgaggtgga
     7561 caggcagggc gccgacagga acatctccga ggacgacaag gccgccatca aggcgctgca
     7621 ggaggatgtg cccctgacgc cgcgtccctt cgacctgtgg ggccggcagg tcggcctctc
     7681 ttacgaggag ctcctggaga gggcctacga tctgcgggac cgcaagatca tgcgccgctt
     7741 ctccgccgtg ctctaccacc gcaaggctgg cttcagggct aacgcgatgg gcgtctggcg
     7801 ggtgcccgac gagcgtatcg acgaggtggg cgacgccttc gcccactacc aggccgtctc
     7861 ccactgctac cagcgcccga cctatgaaga ctggccctac tcggtcttct ccatggtgca
     7921 cggccgcagc gtggaagagt gcgagaacgt gctcgccgcc atggccgagg agacgggcct
     7981 gacggactac acctcgctct actcgacccg cgagtacaag aagacacggg tgcgttactt
     8041 cacgccggag atggaggcct gggaacgcct ctacgccggc atattgcgct gaagttagct
     8101 atcagctttc agctattagc cctcagcttt cagccggcag catgagatag ggacgtattg
     8161 ctgagcgcgg ctttgatcgt tgcgctgttt gcggccctgc tcgcggggat cgttgcgggg
     8221 ctcaccgggt tcggactcgc cctgatcagc acccccatct tgctgttcgt ctacgagcct
     8281 cgcacggtcg tcgtgctcac tgtcatcttc tctgttttca tcaccgctgc ggtggtgtgg
     8341 gactcctggc gagaggcgca caggcggctc gcgttggggc ttctcgttcc cgcgctcttc
     8401 ggggtcgtgg tcggtaccgt tgttctggat gcggtcgacc cgaactacat acggcttgcg
     8461 gtgggcgcca tcgtggtctt ctcggccctg ctgctggtca gggatttcag actgccaggg
     8521 gcgaacacgg gctggggcac gctcgtagcc ggctcggcga gcggggcgct cacgacctcg
     8581 acgggcctgg ccgctccacc tatagtcttg ctcctggcct cacgtggcct cccgaaagac
     8641 gagttccgcg ggacgaccgc gctgtacttc ttgcccatga gcatcgcggg gttcgccgtg
     8701 ctcgctgcca ggggcctggt cgaggcccct gagatacccc tcgggctcgc gctcgttccg
     8761 gccgctatca taggcaaggc catcggtacg gcccttctca agcgcacctc cgagaacgct
     8821 tttcagaaca tcaccctggg gctcgtgatc ctgaccggca ccctgggcgt tttgacagcc
     8881 acgtgggcgc ttctgtaact accccagccg cacgccgatg cggccgtcga gagacgaggt
     8941 gcctcggcta gctggacttc gagggatcca gcaccccggc gttctcgagg atggccgcga
     9001 tctgctcttc cgcgccctcg tccgccggga gtgccggacc gcgcacggcg ggggagatcg
     9061 ggacgccgag catgcgcatg gccagcttga tagccccgat gggagggtcg ctgtggacgc
     9121 cgagggtcac gagctccagt acacgccggt gtagccgcgc cgccgtctcg aggtcgcctt
     9181 cccgcgtcga cgccaccagg ttcacgaaca ggtcgggggc
//
LOCUS       DBNGVD010000116         3173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2756731, whole genome shotgun sequence.
ACCESSION   DBNGVD010000116 DBNGVD010000000
VERSION     DBNGVD010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2756731"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="AC16CM_03345"
     CDS             <1..1563
                     /locus_tag="AC16CM_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HIR3138893.1"
                     /translation="ARESVKNHVMGPLERIEFSGPEDFSRDSKDLGVRFNVARTVNQA
                     YAVGREGNVLDRLSERLRASFGGATIPPDIDYKPELARSEVDEIATQVNEQPGEADVK
                     VYGSEVEVSKSRDGYELNPAATMTSLDNAIGDMSGTVSLQGEVLDPDVTTAEAEKAAD
                     KAREALSGPVEITAKDDESWKIEPDKLGAALEVTKQDGTIDVSLDRDGLESALTPVYD
                     DLTVKTVNASYDFDSDGDVMVKPAHFGREVESESLLDDIEGGIFDGQREYQVSTTVDK
                     PTYTTAELQAKKPTELLGSYHTNYTATSDKTQARINNLTTASRAISGTFLAPGEVFSM
                     NDTVSGLAYEEGHVIVNNATSNALGGGLCQVTSTLYNAALYSGLSIVERHPHATQLPY
                     IRPGMDATVWFGDQYGNGELDMKFKNTTDGYILLQEYVSNDNYIYAEVYGVPSDVSVR
                     MSSEPAYMGYDSSKWTTYYTKRENGKVVSREQWDTSYGALFENGKTVPTDEVPVAEVN
                     GDYFGPNIAAAP"
     gene            complement(1839..2918)
                     /locus_tag="AC16CM_03350"
     CDS             complement(1839..2918)
                     /locus_tag="AC16CM_03350"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3138894.1"
                     /translation="MGAYTVREVNDVAPSLWDEWLRGSPGGGHVLQSYAWGEFKRQVG
                     WRPMRLVLERDGEVAGVGQFLLYNTLPVPGALMYCSKGPWLPWDDREAVGAFFDGVAE
                     LARRSGAHTLKIEPEVHEDDAEARALLEGIGFRDARYDLNFDTTITMDLRIPEDELLA
                     NMRKSTRYSVRKAAREGVEVVEPEDFEGAWSTFYDWMEDTAERKSGFTIRRPKEYLHD
                     MMKTMHDAGRGHLFLAVHEGTPLAGVFVFTFGRKYWFMHGASSTEKRTYNPNHLLQWE
                     VMRWAREQGIDYYDMVGIPKPEDRNESDPYYGVYKFKIGFGGDVTDFVGCLDLPLKNS
                     RARAWYELEPAYYRLYYKVKHNVFY"
     gene            complement(2937..>3173)
                     /locus_tag="AC16CM_03355"
     CDS             complement(2937..>3173)
                     /locus_tag="AC16CM_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3138895.1"
                     /translation="LDSFGNNIPLADPEVGDLIGVFYSGAYGYSASNLGFLSHPSPAE
                     VLLWHGEAHLLRPGGRPEDVLDGQEALSRPGVRG"
BASE COUNT          604 a         1032 c         1012 g          525 t
ORIGIN      
        1 gcgcgagaga gcgtaaagaa ccacgtcatg ggcccgctcg agaggatcga gttcagcggt
       61 ccggaggact tctcccgcga ctccaaagac cttggcgtgc gcttcaacgt cgccagaacc
      121 gtgaaccagg cctacgccgt cgggcgtgag gggaacgtcc tggaccgcct ctccgagcgc
      181 ctgcgggcct ctttcggcgg ggcgacgata ccgcctgaca tcgactacaa gcccgagctg
      241 gcccgctccg aggtggatga gatcgcgacg caggtaaacg agcagccggg agaggcggac
      301 gtgaaggtct acggctcgga ggtagaggtc tccaagagcc gcgacggata cgagctgaac
      361 cccgccgcca cgatgacgag cctcgacaac gctatcggcg acatgagcgg gacggtcagc
      421 ctgcaaggcg aggttctgga ccccgacgtc acgacggccg aggccgagaa ggcggctgac
      481 aaggcccgcg aggccctctc ggggccggta gagataaccg ccaaagacga cgagagctgg
      541 aagatagagc cagacaagct gggagccgcc ctggaggtca ccaaacagga cggcacgata
      601 gacgttagcc tcgaccgtga tggcctggaa agcgccctca cccccgtcta cgacgacctg
      661 acggtcaaga ccgtgaacgc cagctacgac ttcgactccg acggtgacgt catggtcaag
      721 ccggcccact tcgggcgcga ggtggagagc gaaagcctgc tcgacgatat agagggcggc
      781 atcttcgacg gccagcgcga gtaccaggtc tcgacgaccg tcgacaagcc cacctacacc
      841 acggccgagc tccaggcaaa gaaaccgacc gagttgctcg gctcctacca caccaactac
      901 acggccacct cagacaagac ccaggcgcgc atcaacaacc tcaccaccgc ctcgcgcgcc
      961 ataagcggca cgttcctggc cccgggagag gtcttctcca tgaacgacac cgtctcaggc
     1021 ctggcctatg aggagggcca cgtcatcgtg aacaacgcga cgagtaatgc tctgggcggc
     1081 ggtctgtgcc aggtaacctc cacgctgtac aacgctgcac tctactccgg tctcagcatc
     1141 gtggagcgcc acccgcacgc cacccagctt ccgtacataa ggccgggtat ggacgccacg
     1201 gtgtggttcg gtgaccagta cgggaacggc gagctcgaca tgaagttcaa gaacaccacc
     1261 gatggctata tcctcctgca ggagtacgtg tcgaacgata actacatcta cgccgaggtc
     1321 tacggcgtac ccagcgacgt gagcgtgagg atgtcttcgg agccagcgta catgggctat
     1381 gactcttcca agtggaccac ctactacacg aagagggaga acggcaaggt ggtcagcagg
     1441 gagcagtggg acacctccta cggagccctc ttcgagaacg gcaagacggt ccccacggac
     1501 gaagtaccgg tagcagaggt caacggggac tacttcggcc cgaacatagc cgcagccccg
     1561 tagggcacgc tccggcttcg cctccgcgtg ccgcccgccg ccctgcgggc ggcttgtcag
     1621 cccactcggg ctagcgccct cgcttgtcag cacgctccag cctggcggct gtcgctcgtc
     1681 agcaacgctt cggcttcgcc tcgctttcgg ccagtcgtct cgattcgcaa cggtagcttc
     1741 gacatagggc tttcccgggc agcgaatctt tagagcggaa gggtgcaggc cgcaacggga
     1801 tctggctgtc aagcggaggc ccgaagggcc gcagcgtgct agtaaaacac gttgtgtttt
     1861 actttgtaat agaggcggta gtaggctggc tcgagctcgt accaggccct ggcgcgggag
     1921 tttttcaggg ggaggtccag gcagcccacg aagtccgtta cgtcgccgcc gaagccgatt
     1981 ttgaacttgt aaacgccgta gtaggggtct gattcgtttc ggtcttcggg cttggggatg
     2041 ccgaccatgt cgtagtagtc gatgccctgt tctctggccc agcgcatcac ctcccactgc
     2101 agcaggtggt tggggttgta ggtgcgcttc tcggtgctcg aagccccgtg catgaaccag
     2161 tatttacggc cgaaggtgaa gacgaagacg ccggctaggg gcgtgccctc gtgcacggcg
     2221 aggaacaggt ggccccgccc agcgtcgtgc atggtcttca tcatgtcgtg caggtactcc
     2281 ttcggccgcc tgatcgtgaa gccggacttg cgctcggccg tgtcctccat ccagtcgtag
     2341 aacgtgctcc aggccccttc gaagtcctcg ggctcgacca cctcgacgcc ctccctggcg
     2401 gccttgcgga cgctgtagcg cgtggacttc ctcatgttcg cgagcagctc gtcctcgggg
     2461 atgcggaggt ccatggtgat ggtggtgtcg aagttgaggt cgtagcgggc gtcccgaaag
     2521 ccgatgcctt cgagcagggc ccttgcctcc gcgtcgtcct cgtgaacctc gggctcgatc
     2581 ttcagggtgt gcgcgcccga gcgccttgcc agctcggcca cgccgtcgaa gaaggcaccg
     2641 acggcctccc tgtcgtccca gggcagccag gggcccttgg agcaatacat cagcgctccg
     2701 gggacgggta gggtgttgta gaggaggaac tggcccacgc cggccacctc gccatccctc
     2761 tccagcacca gccgcatggg ccgccagccg acctgccgct tgaactcccc ccaggcgtaa
     2821 ctctgcagca cgtgaccgcc gcccggcgag cccctgagcc actcgtccca cagggagggg
     2881 gctacgtcgt ttacctccct gaccgtgtag gcacccaaaa ccctaccgag ccccctctag
     2941 cctcgtacgc cgggcctgga gagggcctcc tggccatcca ggacgtcttc gggcctgccg
     3001 ccggggcgca gcaggtgtgc ctcgccgtgc cacagcagca cctcggcggg ggaggggtgg
     3061 ctcaggaatc ccaggttgga ggctgagtag ccgtatgcgc cgctgtagaa cacgcctatc
     3121 aggtcgccga cctcggggtc ggccagcggg atgttgttgc cgaacgagtc aag
//
LOCUS       DBNGVD010000117         8445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4944062, whole genome shotgun sequence.
ACCESSION   DBNGVD010000117 DBNGVD010000000
VERSION     DBNGVD010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4944062"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="AC16CM_03360"
     CDS             <1..939
                     /locus_tag="AC16CM_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138896.1"
                     /translation="SAWAAVKLLTGRKAPRGSVSPALRERTERLPEGLKDGALRLVNG
                     VAAGWLGFLLADRVLADGAQAVGVAAGLGVLAYLLPRLGALGVIVALTVMLVTNGAGL
                     GFSAIVPVLGGAWVMGAGSIDDDVRKLPLGPALAVPLALLFGLGTVLGSALVAGVPLL
                     FGALMRPLGACLSAAAGALTLICYDLTVRNGFFADGSPYAQLPYSGARLDVLPISSGV
                     GVVLEYAQGYLQYFPKLPLLVLLWAAMAGVVALGEWSGKWLVGLAVAMVGGALGYAML
                     VSQRAPQGTLASDMISLGLAAIIYGVLRYLAMRVRE"
     gene            946..1773
                     /locus_tag="AC16CM_03365"
     CDS             946..1773
                     /locus_tag="AC16CM_03365"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR3138897.1"
                     /translation="MGFLKHIEKRMESLVEGVFGRAFRRQIHPVEIAKGLTKQMDEGR
                     MVSISRTYAPNDFTVHLSREDTESIQAYQASLRDELIQYASTHAENKNYHLMTPPRIR
                     FESEETLRFGEFGVTAKLTGGDGPREKGAPQDTSGQTRIFRTDEASDGEMEQGTAAIS
                     ADEARRHGLAREIVELALEDDVHALAGRGPWGVGRSQENDIVINDPNVSRRHARISRS
                     DNGFVVEDLGSTNGTMLDGAPIDREMIEGGDELTFGQTTARFIRRIDSPGEESGGRR"
     gene            1777..2256
                     /locus_tag="AC16CM_03370"
     CDS             1777..2256
                     /locus_tag="AC16CM_03370"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3138898.1"
                     /translation="MLELQVPLLAAKALILLLLFAFIYAVMSRSIGDLRKIPNDEPYV
                     PGQSRDRNGVLRSRANGVPELVVEDSEILAPETRFEIGDGDTSVGRSTGSTIVLKSDD
                     YASGRHAQLTRHGGLLYVEDLGSTNGTFVNGRKTVGATPIRHGDTVRVGSTTFRYEE"
     gene            2261..3493
                     /locus_tag="AC16CM_03375"
     CDS             2261..3493
                     /locus_tag="AC16CM_03375"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR3138899.1"
                     /translation="MPFLELEPFGVTDAGKVRQNNEDALLIGDGEDETLFVVADGIGG
                     FEAGEVASSLAVEVLRELRPDRPFKSAITEANRRILAAGRTDEKLSGMGTTVVAIRFG
                     GTQQEPLAEVAHVGDSRAYLLRGGEMNPITEDHSLVAELVRSGDLTRDQAAEHPQKNL
                     ITRALGADEDVAVDTTVLPIEAGDRVLLCSDGLSDMVPEARISELLLESPEDPEGTAR
                     SLLAAALDAGGNDNITVIVVDVKEQAPPPRRVRRQSERRSGTSEMNAVELPAQRPEAR
                     PPPPTRESSRRESPRRAQDRARRHQKAARRKRKSGFRRVLAALVRGLAILAFLAIALV
                     PAYLWGSSRYYFAFEGGDVVAYEGLPYAPLGLELNRVWRRPGISEAEIKGPYREPIEN
                     HKLYTESQAERILGDLGS"
     gene            3506..4816
                     /locus_tag="AC16CM_03380"
     CDS             3506..4816
                     /locus_tag="AC16CM_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3138900.1"
                     /translation="MTQRAFTPMVLALLVTTLGFATLIFVQEATSPPLVYGAYYAGTL
                     FALYAGVRLLLPHSDVLLLPIVTLLTGLGLVMVFRLTYDVEGAETLALTQAVWILVGS
                     GTMFLLVLFFRNYDRLFDYKYLLAIFAVFLIASTFTPLGYTVNGARLWVDLGPVGFQP
                     SEFARIALVVFFAGYLAEKRDVMAATSRSFLGVELPAIKYFGPVALVWAASLGLLVFE
                     KDLGSSLLFFGVPLLMLYVATGRIAYILLGGLLFCLGSFVTFLLFDHVQVRVQSWLDP
                     WRDPDGTGFQILQSIFNIADGGLLGTGLGAGFAQTIPEVETDFVFSAVASELGLLGAT
                     AVLLAFLAFTYRGTKIALLAEDDASKLLAFGLTAMFALQTLIIVGGVTKAIPLTGITL
                     PFVSYGGSSVVGNFILTGLLLLVSERAGRREAEGLVYRDERWTS"
     gene            4804..6249
                     /locus_tag="AC16CM_03385"
     CDS             4804..6249
                     /locus_tag="AC16CM_03385"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3138901.1"
                     /translation="MDFVNAQVRRTFYLFAAGFFGLVVVLAYWQVYSREALANNPENG
                     FQTQRSISSPRGLILAGDGETVLARSEKRETPQGPVYDRVYPLGEPFSSVVGYWSTRY
                     GATGIEIANNTDLSSIAGEPQTLDDLINRATGGPQPGNNVVLTLDAGLQELAYQELQQ
                     SITGRGSIVAINPKNGDILAMVSSPSFDANDIDDNYPELASTPNAPLINRATQSLYPP
                     GSVFKVITAAAALEAGVRPQDEFFDSGAYELPGYTVTNFQGKDFGKVTFTQALAYSIN
                     VVFAKLAVEILGADALQQMAENFGFGSTYEDFPLPVATSTVNSLPPNQWTTGTLAQTS
                     FGQGEVQTNSLQMALVTAAIANDGRMVEPRLVREVRSPDGIILDKPTASIRTRSLPPD
                     TAGTLNQMMQQVIIQGGLTEAQIEGVKVAGKTGTAESGNGEAHSWWITFAPADDPKIA
                     MCVMVENGGRADEGALPVAARLMQAYLKEGV"
     gene            6285..>8445
                     /locus_tag="AC16CM_03390"
     CDS             6285..>8445
                     /locus_tag="AC16CM_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3138902.1"
                     /translation="MQQLVDNRYRLKRPLGSGGMADVYLAHDDILDRDVALKVMSTRY
                     KGDEEFVERFKREAQSAAALSHPNIVSIFDRGESEDGTYYIAMEYLPGGTLKDRILSR
                     GALPAHTAAAVALQMAEALRAAHERDVIHRDIKPHNILVTGSGDVKVTDFGIARAASS
                     STMTRTGHILGTAHYISPEQAMGEHVGPASDLYSLGIVLYEMLTGELPFDADTPIGIA
                     MKHVNGHVRPPQELNPSVPDGINAITLRLLAKNPDDRYGSDAELIDDLERFGAGLDPS
                     KATTELMTRAMPAAAAETTRMGATPPPRVAEAGKKRSRRFVPLLLVLLGLLLLAGLGW
                     GGYSLLRGGTAEKEVPRIAVPGLVGMNLEGAQDKYGDDFDIEVSDRIEGEKPVGTITQ
                     QDPSTGKADKNSEISVTVVGTRVASVPGVVGSERAAAEQELKEAGFEVEVKEQESSFG
                     DEGKVVSQDPEGGARPEVGSGVTITVGTGPSSVDVPQLYGITPAQARSVLEEANLELG
                     ERSREYNTDVAEGSIFFQDPSDTERVEPGTAVTVTVSLGVEPVEIPEVYGLSLAQAQA
                     TLSGVGLNSNPVEVEGDEAAGTALSTDPGVGATLAPGSAVTLYYSAGPPPVTTASPAA
                     SSPEATAPPDDEEQPEATPVPQETQELQVIPGQQDRGGNANQPGGQGGSGSGGGGSGD
                     GVSGGGGSGGGGSGGSGSGGSGSGGGGGSGGGGSGGGG"
BASE COUNT         1448 a         2793 c         2858 g         1346 t
ORIGIN      
        1 agcgcgtggg ccgccgtcaa gctgctgacg gggaggaagg ccccgagggg tagcgtctcc
       61 ccggcgctgc gcgagagaac cgagaggctc ccggagggcc tgaaggacgg ggcattacgt
      121 ctcgtcaacg gcgtggccgc gggctggctc gggttcctgc tcgctgacag ggtcctcgcc
      181 gacggcgcgc aggccgtcgg cgtcgccgcc gggctcggcg tgcttgccta cctgctgccg
      241 cgcctcggcg cgctcggggt catagtcgcg ctgaccgtaa tgctcgtgac caacggggca
      301 ggcctcgggt tctccgccat cgtgcccgtg cttgggggcg catgggtgat gggcgccggc
      361 tctatagacg acgacgtgcg gaagctgccg cttggtccgg ccctcgctgt acccctggcc
      421 ctgctcttcg gcctcggaac ggtcctcggg agcgcgcttg tggccggtgt gccccttctc
      481 ttcggcgcgc tcatgcgccc cctgggagcg tgcctctcgg cggcggccgg ggccctgacg
      541 ttgatctgct acgacctcac ggttcgcaac ggcttcttcg ccgacggtag cccctacgcc
      601 cagcttccct actcgggggc gaggctcgac gtgttgccga tatcctccgg ggttggcgtg
      661 gtgctcgagt acgcccaggg gtatctgcag tacttcccca aactgccgct tctcgtgctg
      721 ctgtgggcgg cgatggccgg ggtcgtcgcg ctcggcgagt ggagcgggaa gtggttggtt
      781 gggctggcgg tcgcgatggt cggcggggcg ctcggttatg ctatgctcgt ctcccagaga
      841 gcgccccagg gaacgctcgc ttcggatatg atttcgttag ggctcgcggc tataatatac
      901 ggggtgctca ggtacctggc gatgagggtg agagagtagg tctttttggg attcctcaaa
      961 cacatcgaga agaggatgga gtcgctggtc gagggcgtct tcggaagggc gttcaggcgc
     1021 cagatccacc cggtggagat cgccaagggc ctgaccaagc agatggatga gggcaggatg
     1081 gtctcgatct cccgcaccta cgcccccaac gacttcacgg tccacctctc cagggaggac
     1141 accgagtcca tccaggccta ccaggcctcc ctgagggatg agctgatcca gtacgcctcc
     1201 acccacgccg agaacaagaa ctaccacctg atgaccccgc cgaggatacg cttcgagtcg
     1261 gaagagaccc tccgcttcgg ggagttcggc gtaacggcca agctgacggg cggcgacggc
     1321 ccgcgcgaga agggggcccc gcaggacacc tcgggccaga cccgcatctt ccgcaccgac
     1381 gaggcatccg acggcgagat ggagcagggc acagccgcca tctcggccga cgaggcaagg
     1441 cgtcacggcc tcgcgcgcga gatcgtagag ctcgccctgg aagacgacgt gcacgccctc
     1501 gcaggccggg gaccgtgggg ggttgggcgt tcgcaggaga acgacatcgt cataaacgac
     1561 cccaacgtgt cgaggaggca cgccaggatc tcgcgctccg acaacggctt cgtcgtcgag
     1621 gacctcggtt cgaccaacgg gaccatgctg gacggggcgc ccatcgaccg cgagatgatc
     1681 gaaggcggcg acgagctgac cttcggccag accacagccc gcttcatccg ccgcatagac
     1741 tctccgggcg aggagtctgg cgggagacgc tagcgcatgc tagagctaca ggtgccgctc
     1801 ctggcggcca aggccctgat cctcctgttg ctcttcgctt tcatctacgc cgtgatgagc
     1861 cgcagcatcg gggacctcag aaagatccca aacgacgagc cctacgtgcc tgggcagagc
     1921 agggaccgta acggcgtctt gaggtcccgc gccaacggcg tccccgagtt ggtggtcgag
     1981 gactcggaga tcctggcccc cgagacacgc ttcgagatcg gggacggcga tacgagcgtg
     2041 ggccgctcga cgggtagcac gatcgtgctg aagagcgacg actacgcctc ggggcgccac
     2101 gcgcaactga ccaggcatgg cggcctgctc tacgtggagg atctgggctc gacgaacggc
     2161 accttcgtga acggacgcaa gaccgtgggt gccacgccaa tccggcacgg cgacaccgtg
     2221 agggtcggct cgaccacgtt caggtacgag gagtagcggc ttgcccttcc tggagctaga
     2281 gccgttcggc gtcaccgatg ccggcaaggt ccgccagaac aacgaggacg ccctgctgat
     2341 cggcgatggc gaggatgaga cgctcttcgt ggtcgcggac ggcatcgggg gcttcgaagc
     2401 gggcgaggtt gcgagctctc tggccgttga ggtgctgagg gagcttcgcc cggacaggcc
     2461 tttcaagagc gccataacgg aggcgaaccg caggatactg gccgcaggcc gcaccgacga
     2521 gaagctctct gggatgggta ccacggttgt cgccataagg ttcggcggca cgcagcagga
     2581 gccgctggcc gaggtggccc acgtgggaga ctcgcgggcc tacctcctcc gcggtggcga
     2641 gatgaacccc ataacggagg accactcgct cgtcgccgag ctggtcagga gcggcgacct
     2701 gaccagggac caggcggccg agcacccgca gaaaaacctc atcaccaggg cccttggggc
     2761 cgacgaggac gtcgccgtcg acaccaccgt gctccccata gaggcgggcg accgcgtgct
     2821 gctgtgctct gatggcctct cggacatggt ccccgaggcg aggatctcgg agctcctgct
     2881 cgagtccccg gaggaccccg aggggacggc acgaagcctc ctcgccgcgg ctctggacgc
     2941 cggcggcaac gacaacatca cggtgatcgt ggtcgacgtg aaggagcagg ccccgccgcc
     3001 ccgccgcgtg cgccgccaga gcgagcggag gtcaggcacc agcgagatga acgccgtgga
     3061 actgccggcg cagcggccgg aggcccgccc gccgcctccc accagggagt cttcccgtag
     3121 ggagtccccc cgcagggccc aggaccgggc taggcggcac cagaaggccg cgcgccgcaa
     3181 gcgcaagagc ggcttcaggc gggtgctcgc cgccctcgtc agggggctcg cgatcctggc
     3241 gtttctggct atcgcgctcg tacctgccta cctctggggg tccagtcgct actacttcgc
     3301 tttcgaggga ggggacgtgg tggcttacga gggcctgccg tacgcgcccc ttggtctgga
     3361 gctcaaccgg gtatggcgcc ggcccgggat cagcgaggcg gagatcaagg gtccctacag
     3421 ggagccaata gagaaccaca agctttacac cgagagccag gccgagagaa tcctcgggga
     3481 cctggggagt tagggtgatc ctgcggtgac ccagcgcgcc ttcacgccca tggtactggc
     3541 cctgctcgtt accacgctcg ggttcgccac cctcatcttc gtgcaggagg cgacgagccc
     3601 gccgcttgtc tacggcgcct actacgccgg taccctcttc gcgctctacg ccggggtgag
     3661 gcttctcctg ccccactcgg acgtgctcct gcttccgata gtgacgctgc tgacggggct
     3721 tggcctggtc atggtcttcc gcctgaccta cgacgtggag ggggccgaga cgctcgctct
     3781 cacgcaggcg gtctggatcc tggtggggag cggcaccatg ttcttgctgg tgctgttttt
     3841 ccgcaactac gaccgcctct tcgactacaa gtacctgctc gccatcttcg cggtcttcct
     3901 tatagcctcg accttcacgc ctctcggcta cacggtcaac ggcgcgcggc tctgggtcga
     3961 ccttggcccc gtcgggttcc agccctcgga gttcgcccgc atcgccctcg tcgtgttctt
     4021 cgccgggtac ctggccgaga agcgcgacgt gatggcggca acgagccgga gttttctcgg
     4081 cgtggagctg ccggcgatca agtacttcgg gccggtggcg ctggtgtggg ccgcctccct
     4141 tggtctcctg gtcttcgaga aggacctcgg ctcgagcctg ctattcttcg gcgtgccgct
     4201 gttgatgctg tacgtagcga cgggccgaat cgcctacatc ctgctcggcg ggctcctgtt
     4261 ctgcttgggc tccttcgtga cgttcctact cttcgaccac gtgcaggttc gggtgcagag
     4321 ctggctcgac ccctggcgcg acccggacgg gacggggttc cagatcctgc agagcatctt
     4381 caacatcgcg gacggcggcc tgctcggcac cgggctcggg gccggcttcg cccagaccat
     4441 cccagaagtt gagaccgact tcgtgttctc ggcggtggca agcgagcttg gcctgctcgg
     4501 cgctacggcc gttttgctcg ccttcctcgc ctttacctac aggggtacca agatagcgct
     4561 cctcgccgag gacgacgcct ccaagctcct cgccttcggg ctgacggcca tgttcgccct
     4621 gcagacgctc atcatcgtcg ggggggtcac caaggccatt ccgctaaccg gcatcacgct
     4681 ccccttcgtc tcctacggtg gctcctcggt agtaggcaac ttcatcctga cgggcctgct
     4741 gttgctcgtc tcggagaggg ctggccgcag ggaggccgag gggctggtct acagggacga
     4801 aaggtggact tcgtgaacgc ccaggtcagg cgcaccttct acctcttcgc cgccggcttc
     4861 ttcggcctcg tggtcgtgct cgcctactgg caggtctact ccagggaggc gctggccaac
     4921 aaccccgaga acgggttcca gacccagagg agcatctcct cgccgcgcgg cctgatcctg
     4981 gccggcgacg gcgagacagt gctggcaagg agcgagaagc gggagacgcc gcaggggccc
     5041 gtctacgacc gcgtctaccc gcttggtgag ccgttctcga gcgtggtcgg ctactggagc
     5101 acccgctacg gggcgaccgg catagagatc gccaacaaca ccgacctctc aagcatcgct
     5161 ggtgagccgc agaccctcga cgacctcata aaccgggcca cggggggtcc gcagccgggc
     5221 aacaacgtgg tcctgacgct cgacgccggg cttcaggagc tcgcctacca ggagctgcag
     5281 caaagcatca cgggcagggg ctccatcgtt gccatcaacc ccaagaacgg ggacatcctc
     5341 gccatggtct cctccccctc tttcgacgcc aacgacatcg acgacaacta cccggagctt
     5401 gccagcactc ccaacgcccc ccttatcaac cgggccaccc agagcctgta cccgcccgga
     5461 tcggtcttca aggttataac cgcggcggcg gcgctcgagg ccggggtcag gccccaggac
     5521 gaatttttcg acagcggggc ctacgagctg ccgggctaca ccgtcaccaa cttccagggc
     5581 aaggatttcg ggaaggtaac cttcacccag gcgctcgcct actccatcaa cgtcgtcttc
     5641 gccaagctcg cggtcgaaat cctgggggcc gacgccctgc agcagatggc cgagaacttc
     5701 gggttcggct ccacctacga ggacttcccc ctgcccgtgg cgacgagcac cgtgaacagc
     5761 ctgccgccga accagtggac cacggggacc ctggcccaga cctccttcgg gcagggtgag
     5821 gtgcagacca actcgctcca gatggccctc gtcaccgcag caatcgccaa cgacggcagg
     5881 atggtggagc cccgcctcgt gcgcgaggtc cgctctcccg acggcataat cctggacaag
     5941 ccaaccgcca gcatcaggac cagatccctg ccgcctgaca ccgcgggtac cctcaaccag
     6001 atgatgcagc aggtcatcat ccagggcggc ctgaccgagg cccagataga gggcgtcaag
     6061 gtcgccggca agacgggcac ggccgagtcg ggcaacgggg aggcacactc gtggtggatc
     6121 accttcgccc ccgccgacga cccgaagatc gccatgtgcg tcatggtgga gaacggcggc
     6181 agggcagatg agggggccct gccggtggcg gcacgcctga tgcaggccta tctgaaggag
     6241 ggagtctgaa cgttgtgggg tctggcgttt atacttcata gttcgtgcag caactcgtag
     6301 acaaccgtta ccggctgaaa agaccgctgg gaagcggcgg tatggccgac gtctacctgg
     6361 cccacgacga tatcctggac cgtgacgtgg ccctcaaggt gatgagcacc cgctacaagg
     6421 gcgacgagga gttcgtcgag cgcttcaagc gggaggccca gagcgccgcc gccctctccc
     6481 acccgaacat cgtctccatc ttcgacaggg gcgagtccga ggacgggacc tattacatcg
     6541 cgatggagta cctgcccggc ggcaccctga aggaccgcat cctgagccgc ggcgcgctgc
     6601 cagcccacac ggcggcggcc gtggcgctcc agatggccga ggccctgagg gccgcgcacg
     6661 agcgggatgt gatccaccgc gacatcaagc cgcacaacat cctggtgacg ggctccggtg
     6721 acgtgaaggt tacggacttc ggcatagcca gggctgcgtc ctcgtcgacc atgacccgca
     6781 cagggcacat cctgggcacg gcccactaca tctcgcccga gcaggcgatg ggcgaacacg
     6841 tgggacccgc gagcgacctc tactcgcttg gcatagtgct ctacgagatg ctgaccggcg
     6901 agctaccctt cgacgccgac acgcctatcg ggatagcgat gaagcacgtg aacgggcacg
     6961 tgcgcccacc gcaagagctg aacccttcgg tcccggacgg cataaacgcc ataacgctga
     7021 ggctgctcgc caagaacccc gacgaccgtt acgggagcga cgccgaactg atagacgacc
     7081 tcgagcgctt cggcgctggc ctcgacccct cgaaagccac gacggagctg atgacccgcg
     7141 ccatgccggc ggcggcagcg gagaccaccc gcatgggcgc aacacccccc ccgcgggtcg
     7201 cggaggctgg caagaagcgg agcagaaggt tcgtgcccct gctgctcgtg ctcctcgggc
     7261 tcctgctcct ggccggcctt ggctggggcg gctacagctt gctccgggga gggacagccg
     7321 agaaggaggt gccgaggatc gccgttccag gcctcgtcgg catgaacctg gagggtgccc
     7381 aggataaata cggggacgac ttcgacatcg aggtatccga caggatcgag ggcgagaaac
     7441 ccgttggcac cataacgcag caggatccct ccaccggaaa agccgacaag aactcggaga
     7501 tctcggtcac cgtggtgggc acccgcgtgg ctagcgtgcc aggcgtcgtc ggctcggagc
     7561 gcgccgcggc cgaacaggag ctgaaggagg caggcttcga ggtggaggtg aaggagcagg
     7621 aaagctcctt cggagacgag gggaaggtcg tcagccagga cccggagggc ggcgcgcggc
     7681 ccgaggtagg gtccggcgtg accatcaccg tgggaacagg gccgtctagc gtggacgttc
     7741 ctcagctata cgggatcacc ccggcgcagg cccgctcggt gctcgaggag gccaatctgg
     7801 agctcggcga gcgaagcagg gaatacaaca ccgatgtggc cgagggcagc atcttcttcc
     7861 aggacccctc ggacacggag agggtggagc cggggaccgc tgtgaccgtt acggtcagcc
     7921 ttggcgtaga gccagtcgag atccccgagg tgtacggcct gagcctcgcc caggctcagg
     7981 cgaccctgtc tggggtcggg ctgaactcca accccgttga ggtggaaggc gacgaggcag
     8041 cgggaaccgc gctctcgacg gacccggggg tcggcgcgac gctcgcaccc ggctcagccg
     8101 tgaccctcta ctacagcgcc ggtcccccac cggttaccac cgcctcaccc gccgcctcct
     8161 cccccgaggc aacagccccg cccgatgatg aggagcaacc cgaagcgacg ccagtgccgc
     8221 aggagacgca ggagctgcag gtgatacccg gacagcaaga ccgaggaggc aacgcgaacc
     8281 aacccggtgg gcagggtggt tccggatcag gcggcggcgg atctggcgac ggtgtatccg
     8341 gtggcggtgg ctccggtggc ggtggctccg gtggtagcgg gtccggtggt agcgggtctg
     8401 gtggtggcgg tggatctggt ggcggtggat ctggtggcgg tggag
//
LOCUS       DBNGVD010000118         7610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2393051, whole genome shotgun sequence.
ACCESSION   DBNGVD010000118 DBNGVD010000000
VERSION     DBNGVD010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2393051"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC16CM_03395"
     CDS             complement(<1..917)
                     /locus_tag="AC16CM_03395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018824865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3138903.1"
                     /translation="MEMRRIGSLEVSVVGVGCNNFGWRLDAGATAEVVEAALDAGITF
                     FDTADSYGSTRSEGFLGQALGKRRDGVVIATKFGSRIDERRKGARPEYVRKAVEDSLR
                     RLGTDRIDLYQLHWPDPEVPIGETLGALDELVQAGKVREIGCSNFSAEQLREAEEAAP
                     AGSARFASVQNQYSIMHREPEREVIPECERLDLALIPYYPIANGLLTGKYRRGQPMPE
                     GTRIAEGDRYSDLISEQNLATVESLIEFAESRGHTMLELAFSWLTSRPTVASVIAGAT
                     SPSQARANAAAAGWALSESEVEEVEKISSG"
     gene            complement(1066..2268)
                     /locus_tag="AC16CM_03400"
     CDS             complement(1066..2268)
                     /locus_tag="AC16CM_03400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3138904.1"
                     /translation="MSRTKKVLIIGGGVAGPVAAMALQRADIESVVYEAYTGGAEDAG
                     SFLTLASNGLDALRTIDAHHLVLERGFPTPRMEIRSGTGKHLGVVPNGGTLLDGTVSH
                     TLKRSDLYRTLRDEAIRRGARVEPGRRLVDAEERPNGGVVARFEDGTEAEGDLLIGAD
                     GVHSRTRRIIDPSAPGARYIPVLNVGGYAGGVRVQAEPETFHMVFGKRAFFGYAIHPS
                     GEVWWFANPPRPDEPTRAELAATGTERWREMLVELFAGDDSPAVEIICASRGRLAGWA
                     TYDLPGVPTWQTGSIIVIGDAAHATSPSSGQGASMAIEDGVVLARCLRDLPDVPQAFA
                     FYERLRRKRVERIVAHGARTSNSKAAGPIGRKIRDLMMPVILKHVANSGSLAWMHDYH
                     IDWGAKVA"
     gene            complement(2265..2759)
                     /locus_tag="AC16CM_03405"
     CDS             complement(2265..2759)
                     /locus_tag="AC16CM_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HIR3138905.1"
                     /translation="MNHETIHQTDEARIRALFGDLLGDWGRGDGEAYGSRFTEDADYV
                     AFDGTHTRGRREISTSHQQLFDKYLQGTRLTGRILSIKFLRPDVALIHATGGTIMRGK
                     AEPSPERASIQTLVAVREGEEWRFVAFHNCRVRPIVSGAAGFLIWALTDRLWRVFASG
                     KEDA"
     gene            complement(2943..3575)
                     /locus_tag="AC16CM_03410"
     CDS             complement(2943..3575)
                     /locus_tag="AC16CM_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3138906.1"
                     /translation="MASKRKVSNPLALAVMALLYERPMHPYEMVTVMRERGKHETVRL
                     RYSSLYSVVSAMEREGLISPRETMREGRRPERKIYEITDAGREEFLTWLRELLREPVK
                     EYTQFAAGLSFLPGLPPEEAIALLEERVGHLEAETGEMRARIDYAREEYALPRLFVVE
                     SEHELMLREAELDWVRGLIEDMRSGELGSLAEWHSILAGRNEGGGDEPMA"
     gene            complement(3683..4411)
                     /locus_tag="AC16CM_03415"
     CDS             complement(3683..4411)
                     /locus_tag="AC16CM_03415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138907.1"
                     /translation="MPEYDSLANVYDLEYAHDYDLPFWLSLAEREAGPVVEWGAGTGR
                     IAVPLAAGHEVTAVEISKEMAERGRQKGSSVGWILGDMRDVKPKRSYGLAVCAFNSFL
                     CLQSTEDALAFLGNAHDHLLPGGLLGIEVSAFSPEELAGGAELRHDFTRELPDGKLDR
                     FSVSTYDAATQLMHMRLFYERYDGSGSFENRMAQDLTIRTTNRDELLLMLRLSGFEAE
                     EVYGDFQGGPFDSRSDHLIVLARK"
     gene            4453..4827
                     /locus_tag="AC16CM_03420"
     CDS             4453..4827
                     /locus_tag="AC16CM_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3138908.1"
                     /translation="MITALDHVQLPMPEGREEDARDFFGRLLALEEIQKPPSLARRGG
                     VWFAVPDGRQIHLGVEEPFRPNRKAHPAFVCAVLEDLAQRLGARGHPVRWDGELAPRR
                     RFYSEDPFGNRLEFLEPVSEQA"
     gene            4892..5452
                     /locus_tag="AC16CM_03425"
     CDS             4892..5452
                     /locus_tag="AC16CM_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3138909.1"
                     /translation="MGIVLIIILVVIAVVLVIYAISTYNGLVRRRNEVEESYRQIDVQ
                     LKRRYDLIPNLLEGVKKYFRQEQDVLTQITQARSQAMQPQSPGEQAQSEARLSGAIGN
                     LFAVAESYPDLRSNTVLIDFQEELSSTENKISFARQHYNDSVQSYNTKIQSFPAVIFA
                     RAMGFTEREYFEAQGPERESVTVSYD"
     gene            5457..6446
                     /locus_tag="AC16CM_03430"
     CDS             5457..6446
                     /locus_tag="AC16CM_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3138910.1"
                     /translation="MEQRDLGTFRERIARNRRNSLLLIAAFLAFITLFGYIIGWAWLG
                     DPVRALGGLVFALVIGIVAGLISYYGGDKMVLAASRAREITHDDAPVLFNVVEEMSIA
                     AGLPMPKVYIVDDSAPNAFATGRDPQHAVVAVTSGLLEKLDRDELQGVIAHEMSHVAN
                     FDIRYSMLVGILVGTTVLISDFFLRGLWFGGGRGGGRRGGGDGGGQLQLIMMAIAIVL
                     AILAPLFARLLQLSISRQREFLADATAVRLTRNPKGLADALQKINGDKEVLEAANRAT
                     AHLYIVNPIKRFEKRSKGLFSTHPPIEERIQILRAIEAGGVAETSGQTSTEAP"
     gene            6472..6545
                     /locus_tag="AC16CM_03435"
     tRNA            6472..6545
                     /locus_tag="AC16CM_03435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6504..6506,aa:Gln,seq:ctg)
     gene            complement(6589..7080)
                     /locus_tag="AC16CM_03440"
     CDS             complement(6589..7080)
                     /locus_tag="AC16CM_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138911.1"
                     /translation="MAEEPQRKYLVRAQEISEQAVTASHPWNPNSEFTGTRLAALSGL
                     TRVGVNLMKVPPGKESFIYHSQEREEEWIYLISGRGVAEIEDEEFEVGSGDFMGFPTG
                     VAHHLRNPYDEDLVYLVGGENVDIEVANFPRLGRRIIRKRENVQVYRTSDATDFGELT
                     EDT"
     gene            complement(7149..>7610)
                     /locus_tag="AC16CM_03445"
     CDS             complement(7149..>7610)
                     /locus_tag="AC16CM_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3138912.1"
                     /translation="DKTPAAVRQIAHRARGHVAARRPRVRVDRAEHQRVVDRFLAAVG
                     SGDLQALFDVLAPEVVMVADGGGEVAAIRRPVEGREQVATLLSRFRTVSPDAVVGTAW
                     LNGAPAVRIDMAGEPDTAVSLVVSEGRITHIYAVRNPHKLARLDRETTFSR"
BASE COUNT         1258 a         2580 c         2359 g         1413 t
ORIGIN      
        1 ccggaagaga ttttctcgac ctcttcgacc tccgactcgg agagcgccca acctgcggcc
       61 gcggcgttgg cccttgcctg cgagggggag gtggccccgg cgatcaccga ggcaacggtg
      121 ggccgcgaag tcagccagga gaaggccagc tccagcatag tatgcccccg cgactcggcg
      181 aactcgataa gcgactctac ggtggccagg ttctgctcgg agatgagatc ggagtacctg
      241 tcgccctccg cgatgcgggt accttccggc atgggctgtc cccgtcggta cttgcccgtg
      301 agcaggccat tggcaatggg gtaatacggg atcagtgcga ggtccaggcg ctcgcactcg
      361 gggatcactt cgcgctccgg ttcacggtgc atgatgctgt actggttctg tacgctggcg
      421 aagcgcgccg agccagcggg tgccgcctcc tcggcctcgc gtagctgctc cgcagagaag
      481 ttggaacagc cgatctcgcg caccttgcca gcctgaacga gctcgtcgag tgcgcctagc
      541 gtctcgccga tgggcacctc cggatccggc cagtgcaact ggtagaggtc tatacggtcg
      601 gtgccgagcc tcctgaggct atcctcgacc gcctttcgca cgtactcggg tctggcgccc
      661 ttgcgccgct cgtctatgcg gctgccgaac ttcgtggcga tcaccacccc gtcacgacgc
      721 ttgccgagag cctggccgag aaacccttcg ctcctggtgc tgccgtagct atccgcggta
      781 tcaaagaagg ttatgcccgc gtccaacgcg gcctccacta cctccgcggt tgccccggcg
      841 tcgagacgcc aaccgaagtt gttgcagccg accccgacca cggaaacctc cagagagccg
      901 atgcgcctca tctccatggt gtgccgtcct ctcctgccgt ccgcgtacaa gactttgtgt
      961 gatcctgccc gccccgaaag gcgccagcat cccacagctt tttgcccggt tacgcggcaa
     1021 accacacggg caagcgatag aacgatacac cagccaggtg ccgccttatg ccaccttcgc
     1081 tccccagtcg atgtggtagt cgtgcatcca ggcgagggac ccgctgtttg ccacgtgctt
     1141 caggatcact ggcatcatga ggtctcgtat cttcctgccg atgggacccg cagccttgct
     1201 gttgctggtt ctggccccgt gcgcgacgat gcgctctacc cgcttgcggc ggagccgctc
     1261 gtagaaggcg aaggcctgcg gcacgtccgg caggtcgcgc aggcaacggg cgagcacgac
     1321 gccgtcctcg atggccatgg aagcgccctg tcccgaggag ggcgaagtgg cgtgcgccgc
     1381 gtccccgatc acgataatcg aacccgtctg ccacgtcggg accccgggta gatcgtaggt
     1441 ggcccatcct gcgagccttc cccgggaggc gcagatgatc tcgacggccg gggagtcgtc
     1501 cccggcgaag agttccacca gcatctcccg ccaccgctcc gtgcctgtcg cggcgagctc
     1561 cgcccgcgtt ggctcatcgg ggcggggcgg gttggcgaac caccatacct cgccggaggg
     1621 atggatcgcg taaccgaaga atgcccgttt cccgaagacc atgtggaacg tctcgggttc
     1681 ggcctggacg cgcacgccgc cggcgtaacc gccgacgttc agcaccggga tgtaacgggc
     1741 ccccggcgcg gacgggtcga tgatgcggcg cgtcctggag tggacgccgt cggccccgat
     1801 caggaggtct ccctccgcct ccgttccatc ctcgaaccgt gccacgacgc cgccgttggg
     1861 ccgctcctcc gcgtccacca gccgcctgcc gggctcgacg cgggctccgc ggcggatggc
     1921 ctcgtcgcgc agcgtcctgt agaggtcgga cctcttgagc gtgtggctga ccgtgccgtc
     1981 gagcagggtc ccgccgtttg gaacgacacc gaggtgtttg ccggtgccgc tccgtatctc
     2041 catgcgcggc gtggggaagc cccgctccag tacgaggtgg tgcgcgtcga tggtgcgcag
     2101 ggcgtcgagg ccgttgctcg ccagggttag aaacgaaccg gcgtcctccg cgccacccgt
     2161 gtacgcttcg tagacgaccg actctatatc ggcccgctgc agcgccattg cggccaccgg
     2221 tcctgccacg ccgccgccta tgatgaggac cttcttcgtg cggctcatgc gtcctccttc
     2281 ccggacgcga agacgcgcca gagcctgtcc gtgagggccc agatcaggaa gccggctgcg
     2341 ccactaacta tgggacgcac cctgcagttg tggaacgcga cgaaacgcca ttcctcgccc
     2401 tcgcgcaccg cgacgagggt ctggatggag gcccgttccg gtgaaggctc tgctttgccg
     2461 cgcatgatcg tgccgcccgt cgcgtggatg agggccacgt ccgggcggag aaacttgatg
     2521 ctcaggatac ggcccgtgag ccgcgtgccc tggagatatt tgtcgaagag ttgctggtgg
     2581 gaggtggaga tctcccgcct tcccctggtg tgcgtcccgt cgaaggctac gtagtcggca
     2641 tcctcggtga aacgggaacc gtatgcctcg ccgtcgcccc tgccccagtc gccgagcagg
     2701 tctccgaaaa gagcgcgtat tctcgcctcg tcggtctggt gtatggtctc gtggttcatc
     2761 aggtcgaaag gtccttcctc gtcgcggacg gcaacagagg cgccgtctct ccgcggagct
     2821 tcgacctgtt atccttgaga acgcgggttc tgggtaactg gtctcgcccc gcggggcctg
     2881 ctactcggga ttccgacccc cggtccgagg tgctgcaaca ccacaccggg ggtcatctct
     2941 gctcaagcca ttggctcgtc acctccgccc tcattgcgcc ccgcgaggat cgagtgccac
     3001 tccgcgaggc tacccagctc tcccgacctc atgtcctcta tgagcccgcg cacccaatcc
     3061 agctcggcct cccgcaacat gagctcgtgc tccgactcca ccacgaagag acggggcagg
     3121 gcgtactcct ccctggcgta gtcgatccgg gctctcatct ccccggtctc cgcctccagg
     3181 tgcccaaccc tttcctccag gagcgcaatg gcttcctcgg ggggcaaccc gggaaggaaa
     3241 gagaggccgg ccgcgaactg cgtgtactcc ttgaccggct cccgcaacag ctcgcgcaac
     3301 caggtcagga actcctcccg cccggcgtcc gtgatctcgt agatcttgcg ctccggccgt
     3361 ctcccctcac gcatcgtctc cctgggcgag ataagacctt ccctctccat cgccgaaaca
     3421 accgagtaca gcgagctgta cctgagccgc accgtctcgt gcttgccccg ctcacgcatc
     3481 acggtcacca tctcgtaagg gtgcatcggc cgctcgtaga gaagcgccat caccgccagc
     3541 gccagcgggt tggagacctt cctcttcgat gccaccctct ctccttaatc gtaatcgatt
     3601 agtcgagaat gactatatag gtaggatctc tgcttgtcaa gacttcggct ctggaggcgg
     3661 cgttctgctt cgaggtcggg gtctacttcc tggccagcac gatgaggtga tcgctcctcg
     3721 agtcgaaggg accgccctgg aagtcgccgt agacctcctc ggcctcgaag cccgagagcc
     3781 tgagcatgag cagcagctcg tcgcggtttg tcgtcctgat ggtgaggtct tgcgccatgc
     3841 ggttctcgaa ggagcccgag ccgtcgtacc gttcgtagaa caggcgcatg tgcatcagct
     3901 gggtggccgc gtcgtaggtg gagacggaga agcggtcgag cttgccgtcg ggaagctcgc
     3961 gcgtgaagtc gtgccgcagc tccgcgccgc ccgccagctc ctccggcgag aacgccgaga
     4021 cctcaatgcc aagcaggccg cctggcagca ggtgatcgtg ggcattgcca aggaacgcca
     4081 gggcgtcttc cgtgctctgc aggcacagaa acgagttgaa ggcgcacacg gcgaggccgt
     4141 agcttcgttt cggcttcacg tctcgcatat cgccaaggat ccagcctacg gaagagccct
     4201 tctgtcgacc cctctcggcc atctccttgg agatctcgac ggcggttact tcgtggccgg
     4261 cggctagtgg gacggctatt cgtcccgtgc ccgcgcccca ttccactacg ggtccggcct
     4321 cgcgttcggc cagcgagagc cagaacggaa ggtcgtagtc gtgggcgtac tccaggtcgt
     4381 atacgttggc cagagagtcg tattctggca agggttgctc cctccgcgca tcgggcctat
     4441 ccttcgtccg gcatgataac cgctctggac cacgttcagc tcccgatgcc ggagggccgg
     4501 gaggaagacg ccagggactt cttcggccgg ctgttggcgc tggaggagat ccagaagcct
     4561 ccgtccctgg cgaggagggg cggggtctgg ttcgccgtcc ctgacgggcg tcagatccac
     4621 ctcggggtcg aggagccgtt ccggcccaac cggaaggctc atcccgcgtt cgtgtgcgct
     4681 gtgctcgaag atctcgcgca gaggctcggg gctcgtggtc atccggtgcg gtgggatgga
     4741 gagctggcgc cccggcgcag gttctactcc gaggatccgt tcgggaaccg gttggagttt
     4801 ctcgaaccgg tttccgagca agcatgaatc tttgaccctc ccggcggcgt ataatcagga
     4861 gagacaacga ctagtcgcgg aggattgaag tatgggcatc gtcttgatca ttattctggt
     4921 cgtgatcgcg gtggtgttgg ttatctacgc catctccacc tacaacgggc tcgtgcggcg
     4981 gcgcaacgag gtcgaggagt cttatcggca gatagacgtg cagctaaagc gccgctacga
     5041 tctgatccca aacctcctcg aaggcgtgaa gaagtacttt cgtcaggagc aggacgtgct
     5101 gacccagata acgcaggccc gctcccaggc catgcagccc cagtcgccgg gcgagcaggc
     5161 ccagtccgag gcccggctgt cgggtgccat aggcaacctg ttcgcggtgg ccgagagcta
     5221 cccggacctc cggtcgaaca cggttctcat agacttccag gaggagctct cctcgacgga
     5281 gaacaagatc tccttcgcca ggcaacacta caacgactcc gtccagtcct acaacaccaa
     5341 aatacagagc ttcccggccg tgatcttcgc ccgcgccatg ggcttcacgg agagggagta
     5401 cttcgaggcc cagggccccg agcgggagtc cgtgacggtc tcctacgatt gagatcatgg
     5461 aacagaggga cctcggcacc ttcagggagc gaatcgcccg caacaggcgc aacagcctgc
     5521 tgctcatcgc cgcctttctg gcgttcatca ccctcttcgg ctacatcata ggatgggcct
     5581 ggctcggtga ccccgtgagg gcgctcggcg gtctcgtctt cgccctggtg atcgggatcg
     5641 ttgccggcct cataagctac tacgggggcg acaagatggt gctcgccgct tcgagggcca
     5701 gggagatcac ccacgacgac gcccccgtcc tgttcaacgt ggtggaggag atgtccatag
     5761 ccgccggcct ccccatgccc aaggtctaca tcgtcgacga ctcggccccc aacgccttcg
     5821 ccacaggccg cgacccccag cacgccgtgg tcgccgtcac cagcggcctg ctcgagaaac
     5881 tcgaccgcga cgagcttcag ggtgtgatcg cccacgagat gtcccacgtc gccaacttcg
     5941 acatccgcta ctccatgctc gtcggcatcc tggtcggcac gaccgtcctg atctccgact
     6001 tcttcctgcg cggcctgtgg ttcggcggcg gaaggggcgg aggacgccgg ggaggggggg
     6061 acggcggcgg acagctgcag ctcattatga tggccatcgc catcgtgctt gccatcctcg
     6121 cgcccctgtt cgcccgcctc ctgcagctct ccataagccg ccagcgcgag ttcctggccg
     6181 acgcgacggc cgtcaggctc acgcgcaacc ccaagggcct cgccgacgcg ctacagaaga
     6241 tcaacggcga caaagaggtt ctggaggccg ccaaccgtgc cacggcccac ctctacatcg
     6301 tcaaccccat caagaggttc gagaagcgct ccaagggcct cttctcaacc cacccgccta
     6361 tcgaggagag aatccagata ctccgcgcca tcgaggccgg cggcgtcgcc gaaacctcgg
     6421 gacagacctc gacggaggcc ccttaaagcg ttacaatatc cgccgttaca ttgccggatg
     6481 gtgtaacggc agcacgagtg actctgaatc acttagtcct ggttcgaatc caggtccggc
     6541 agccacccga ggatcctgca agcgggagac ctctccttcc tgggcttctc aggtgtcttc
     6601 ggtcaactcc ccgaagtcgg ttgcgtccga ggttctgtag acctggacgt tttctctctt
     6661 gcggatgatc cgacgcccca gccgcgggaa gttcgccacc tctatgtcca cgttctcccc
     6721 gcccaccagg tagaccaggt cttcgtcgta gggattgcgc aggtggtggg cgacgccggt
     6781 tgggaagccc atgaagtctc cggaaccgac ctcgaactcc tcgtcctcta tctccgcgac
     6841 cccgcgtccg gagatgaggt agatccactc ctcctcccgt tcctgggagt ggtagatgaa
     6901 ggactccttg cctggtggca ccttcatgag gttgacgccc acccgcgtta gcccggacag
     6961 ggcggcgagc cgggtgccag tgaactcgga attggggttc cagggatggg aggctgtaac
     7021 cgcctgttcg gagatctcct gcgccctcac caggtatttc ctctgcggtt cttctgccac
     7081 cgtgatctcc tccctgttcc ggacctggtg gaagtatcca acgcgggcat cgtctttgcc
     7141 acccggggtc agcggctgaa ggtggtctct ctgtccagac gggcgagctt gtgcgggtta
     7201 cggaccgcgt agatgtgggt gatacggccc tcggacacca ccaggctgac cgccgtatct
     7261 ggttcgccgg ccatatctat gcgtacggcc ggtgcgccgt tcaaccatgc ggtgccgacc
     7321 accgcatccg gcgagacggt cctgaagcgt gagagcaagg tcgccacctg ctcgcggccc
     7381 tctaccggac gccggatggc ggccacctcg ccgccgccgt cggcgaccat caccacctcg
     7441 ggtgctagca cgtcgaacag ggcctgcaga tctccgctcc cgaccgccgc caggaatcgg
     7501 tctaccaccc tctgatgctc ggcacgatct accctcaccc gcggtctacg ggcggccacg
     7561 tgtcctctgg cgcggtgggc gatctgccgg accgccgcgg gcgtcttgtc
//
LOCUS       DBNGVD010000119         4490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1846088, whole genome shotgun sequence.
ACCESSION   DBNGVD010000119 DBNGVD010000000
VERSION     DBNGVD010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1846088"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC16CM_03450"
     CDS             complement(<1..358)
                     /locus_tag="AC16CM_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138913.1"
                     /translation="MVGTIFLAVAAISLWTFFAGLSLTHATLLVGIALGVVSAGARFM
                     PIGFGVGRASFDAGGVPIFAALVLGGPACALLAAVPSAVHRDPTRTGFMAATHVLQVV
                     AGALAFALFYPQPLLLD"
     gene            940..1203
                     /locus_tag="AC16CM_03455"
     CDS             940..1203
                     /locus_tag="AC16CM_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138914.1"
                     /translation="MRTFSLLFAAIALVMLTIFVIYIARSPTDPVIAGGLFVSFLACV
                     IPALLLLKPYEPPEALQRRREEARRRREERRRSSDGPGSGSGS"
     gene            complement(1169..1615)
                     /gene="rimI"
                     /locus_tag="AC16CM_03460"
     CDS             complement(1169..1615)
                     /gene="rimI"
                     /locus_tag="AC16CM_03460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3138915.1"
                     /translation="MSGPHLRRMGEGDLSSVMEIDALCLPRPWSAAIWRGELTSPYGL
                     YLVIEDNGEVSGHIGVRHVLGELHITTIAVRPEHRRRGHARALIGAALAAYPRATHVH
                     LEVRPTNEGAISLYESLGFEATGSRPRYYGDEDALLMTLNLNQDRH"
     gene            complement(1612..2295)
                     /gene="tsaB"
                     /locus_tag="AC16CM_03465"
     CDS             complement(1612..2295)
                     /gene="tsaB"
                     /locus_tag="AC16CM_03465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3138916.1"
                     /translation="MLILALDASTPVTTVALGRGDLHGGASAREVLAEVSTRGRGASE
                     TLLPAVQTALDLCGEELHSVDLVLAGVGPGTFTGIRIAAATARALSVGSGIALARNST
                     LDALAVPALSCCPDVLALLDARRGQVFARRYSGTGPTTGIRCAGPEELSVGGEPLLVG
                     DGAVRYREVLAGLGHIPPDGSPLHRVSAAGHVLSADLTPVGPEELVPIYVREPDAEVR
                     RDLNPWSRP"
     gene            complement(2289..2738)
                     /gene="tsaE"
                     /locus_tag="AC16CM_03470"
     CDS             complement(2289..2738)
                     /gene="tsaE"
                     /locus_tag="AC16CM_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3138917.1"
                     /translation="MTFEGLEEAALQVLASKVARALVPGDVVVLSGEVGSGKTTFVRA
                     AALALGVTERVTSPTYQLARGYEGLVDGRRVVVNHMDLYRLEGIEDRDALELDDYLDP
                     EAVTFIEWAEPALDAIDDPSLVRISHETPTTRGVSLDGPIEERLSSC"
     gene            complement(2754..3377)
                     /locus_tag="AC16CM_03475"
     CDS             complement(2754..3377)
                     /locus_tag="AC16CM_03475"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3138918.1"
                     /translation="MEAQKEAMGCSKCGLCHSRTQVVFGEGPLNAALFVVGEAPGFNE
                     DREGRPFQGASGLLLDRLLESLGFARERVYLTTLNKCRPPGSPPRSPKPSEVSSCRPY
                     LVAQLEAVDPKVVVALGDLPSRVLTGKKESVSRIRGRAVPVDGRYVFPTLSLQRALYT
                     PADRALFISDFSRLPELLEAERPSSYETLNVTRSPEAEREPVQPGLW"
     gene            complement(3390..3590)
                     /locus_tag="AC16CM_03480"
     CDS             complement(3390..3590)
                     /locus_tag="AC16CM_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3138919.1"
                     /translation="MNIERSRSDRWIRGVCGGVAHTYGWNPNVVRLITVLLAIIIPGP
                     STVLAFAAYLILSFVLPESEEF"
     gene            complement(3667..4020)
                     /locus_tag="AC16CM_03485"
     CDS             complement(3667..4020)
                     /locus_tag="AC16CM_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138920.1"
                     /translation="MFEGLKWKSAARRSAVVIVIYLGLLYVLGVAFPGSSFSLDDRSQ
                     IISLLVNAVIFFFIFTFVYALIERSRERRTGGAGKQSRPGKPSQNGGDVGADASPLRG
                     RPNPNTSRKKSRRRR"
     gene            complement(4004..>4490)
                     /locus_tag="AC16CM_03490"
     CDS             complement(4004..>4490)
                     /locus_tag="AC16CM_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3138921.1"
                     /translation="DLVRPGVALYGLHPAGDEGDAASENLRPAMSLTSYGASVRRLAS
                     GDGVSYGMTFRAGAPMLAATVPVGYAEGYRRALSGKAEALIRGRRRPLLGRVTMDACV
                     FGVDDAVEVGDEVVLLGTQGVGRVGAEELGSRSGTINYEITTGINPRRVERSYRNVRR
                     T"
BASE COUNT          772 a         1470 c         1510 g          738 t
ORIGIN      
        1 gatccaggag cagcggttgg gggtagaaca gggcgaaggc gagcgcgccg gccacgacct
       61 gcaggacgtg ggtcgccgcc atgaagcccg tgcgggtggg atctcggtgt acggccgagg
      121 gcacggcggc caggagtgcg caggcaggac cacccagaac cagcgcggcg aagatcggca
      181 cgccgcccgc gtcgaaggaa gcccgcccca ccccaaagcc aatgggcatg aagcgtgccc
      241 cggcgctcac gaccccgagc gcgatcccta ccagcagcgt cgcgtgggtc agggagagcc
      301 cggcgaagaa ggtccagagc gaaatggcgg caacggcgag gaagatcgta cccaccacgt
      361 aggcacgggc aagctcgggg cgcttgcggg gcgcacccga agtgttaacg gtggatcacc
      421 accaggaaga cgatctttgg cgctcgtacc atcccgagca ttgtagaaca aagaatctcg
      481 cggggctaca tcttatgcac aacccgacgc gtcctctacg ttttccagtc acgcgctacc
      541 agcacctgcg ggtcatattc gattcacccc ctcacgcaag ccgctgcaac gtcttggtgg
      601 gtattttgca ccattcgtag cgcagataca gcagaaaatg tgaacgaacc gccgatactt
      661 ctggagaaaa gcgcgaaaga cttgccttga tgggccgttc gaccgcatga acgagggttg
      721 gggggacaca gtatctgggg gtgtggaaga cacctgacct cgactacact ggctgcaagg
      781 gccgtcgtcc taccccgggt agaacggggt ccacaagccc ggtcggttct ccccatcctc
      841 ccccgactgg gctttttcgc gtgtgcacac cggaccaggt atgccagggg acgatcacgc
      901 ttgcggtccc ttgtcagggt ggcggtctat actctttcca tgcgtacgtt ctcattgctg
      961 ttcgcggcca tcgcgcttgt gatgctcacg atcttcgtga tctacatcgc caggagccct
     1021 acggaccccg tgatcgcggg tggattattc gtctcttttc tggcctgcgt gatccctgcg
     1081 ctgttgctgt tgaagcctta cgagccgcct gaggcgttgc agcgccgaag ggaggaagcc
     1141 cgcaggaggc gcgaggagcg ccggcgctct agtgacggtc ctggttcagg ttcagggtca
     1201 taagcaacgc atcttcgtcg ccgtagtagc gcggtctgct gcccgtcgcc tcgaagccaa
     1261 gcgactcgta gagggagatc gcaccttcgt tggtcggtcg cacttcgaga tgcacgtggg
     1321 tggccctggg gtaggcggcg agcgcggcgc cgatcagggc cctcgcgtgc ccgcggcggc
     1381 ggtgttcggg tctgacggcg atagtggtaa tgtggagctc accgaggacg tggcgcaccc
     1441 cgatatgtcc cgagacctcg ccgttgtctt cgatcaccag gtagaggccg taggggctgg
     1501 taagttcccc gcgccagatc gccgcactcc aaggccgggg caagcacagg gcgtctatct
     1561 ccatcacgga agagaggtcg ccctccccca tcctgcgcag gtgcgggccg ctcaaggacg
     1621 gctccagggg ttgagatccc tcctcacctc ggcgtccggc tcgcgcacgt agatcggcac
     1681 cagctcttcg gggcctacgg gggtgagatc ggcggacagg acgtgcccgg ccgcggagac
     1741 ccggtggagc ggcgagccgt ctggcggtat gtgtccgagc ccggcgagaa cctcacgata
     1801 acgcacggcc ccgtcgccaa cgaggagagg ctctccgccc accgaaagct cttcaggccc
     1861 cgcgcagcgg atgccggtcg tcggccccgt cccggagtac ctgcgggcga aaacctgccc
     1921 ccttctggca tcgagcagcg ctagcacgtc tgggcagcac gagagcgcgg ggacggcgag
     1981 ggcgtccagg gtggagtttc tggcgagggc gatgcccgag cccaccgaga gcgcgcgggc
     2041 cgtggccgcc gctatgcgga tgcccgtgaa cgttcccggt ccgaccccgg cgagcacgag
     2101 gtccacggag tggagttcct ccccgcagag gtccagcgcg gtctgcacgg cggggagcag
     2161 cgtttcggag gcgccgcggc ccctggtgga gacctcggcg aggacctcac gggcgctggc
     2221 gccgccgtgg aggtcgccac ggcccagggc cacggtggtc accggcgtcg aggcgtcgag
     2281 ggcgaggatc agcacgatga caggcgctcc tcgatgggcc catccaggct taccccgcgc
     2341 gtggtcgggg tctcgtgtga gatgcgaacc agcgaggggt catcgatggc gtcgagtgcg
     2401 ggctcggccc attcgataaa ggttactgcc tccggatcca ggtagtcgtc gagttcgagc
     2461 gcgtcgcggt cttcgatccc ctccagccgg tacaggtcca tgtggtttac gaccacacga
     2521 cgtccgtcaa ccagcccctc gtagccgcgg gcgagctggt aggtcgggct ggtcaccctc
     2581 tccgtgacgc caagggcgag ggccgcggcg cgtacgaagg tggtcttgcc cgagcctacc
     2641 tcaccggaga gcactacaac gtcgccaggt acgagggccc gcgccacctt cgacgccagc
     2701 acctgcaggg ctgcctcctc cagaccctcg aacgtcacga gaacttcggg actctaccag
     2761 agccccggct ggacgggctc ccgctcggct tcgggggaac gggtgacgtt cagggtctcg
     2821 tagctggagg gccgctcggc ctcaaggagc tcgggaagcc gcgagaagtc ggagatgaag
     2881 agcgcccggt ctgccggggt gtagagggcg cgctgcaggg agagcgtcgg gaacacgtag
     2941 cgcccgtcca cgggaacggc ccggcccctg atgcgcgaga cggactcttt cttgcccgtg
     3001 agcacccggc tcggcaggtc gcccagggcc acgacgacct tcgggtcgac ggcctcgagc
     3061 tgggcgacga ggtagggccg gcaggagctc acctcggaag gcttgggaga ccgcggcgga
     3121 gacccgggcg gcctgcactt gttcagcgtg gtcaggtaga cccgctccct ggcgaacccc
     3181 agagactcga gcaaacggtc aagtagcagc cccgaagccc cctgaaaggg cctaccctcc
     3241 ctgtcctcgt tgaagccagg ggcctcgcct accacgaaca gggcggcgtt gagggggccc
     3301 tcaccgaaga cgacctgcgt cctggagtgg cagagcccgc acttcgagca gcccatggcc
     3361 tccttctggg cctccacaaa ctccaagccc tagaactcct cgctctcagg caggacgaag
     3421 ctcagtatca ggtaggccgc gaaggcgagc accgtgctcg ggccagggat gatgatggcg
     3481 agcagcacgg tgatgagccg cacgacgttt gggttccacc cgtacgtgtg ggccacgccg
     3541 ccgcagacgc cccggatcca acggtcactc ctcgacctct cgatgttcaa gccgttgcct
     3601 cctcttcgct atcatcgcga ttctacacga cggcggttcg tccaggagga gatgatccct
     3661 ggcgttctag cggcggcggc gggatttctt gcggctcgtg ttggggttgg ggcggcccct
     3721 tagcggggag gcgtcggccc ctacgtcgcc cccgttctgg gatggcttgc ccggtctgct
     3781 ctgtttgcca gccccgcccg tgcggcgttc cctgctgcgt tctatcaggg cgtagacaaa
     3841 cgtgaagatg aagaagaata taacggcgtt tacgagcagg ctgatgatct gggacctgtc
     3901 gtccaggctg aagctggagc cgggaaaggc gacacccagc acgtagagca acccgaggta
     3961 gatcactatg acgaccgccg accgtctggc ggctgatttc cacttaagtc cttcgaacat
     4021 tccggtagct cctctcgacg cgtcgggggt ttatccccgt agtgatttcg tagtttatcg
     4081 tgccggaccg cgagccgagc tcttcggcgc cgacgcgccc gacaccctgg gtgccgagca
     4141 gcacgacctc gtccccgacc tcgacggcgt catctacgcc gaagacgcag gcgtccatcg
     4201 taacccttcc caggagcgga cgcctgcggc ccctgatgag ggcctctgcc tttccggata
     4261 gcgcccgcct gtatccctct gcgtacccca cgggcaccgt ggccgcgagc atcggcgccc
     4321 ccgccctgaa ggtcattccg taggacacac cgtctcccga ggcaagcctc cgcacggagg
     4381 caccgtagct cgtgagcgac atcgcgggcc gcaggttctc cgaggccgcg tctccctcat
     4441 ctccggcggg gtgcaggccg tagagtgcga caccgggccg gacgaggtcg
//
LOCUS       DBNGVD010000120         5840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4944302, whole genome shotgun sequence.
ACCESSION   DBNGVD010000120 DBNGVD010000000
VERSION     DBNGVD010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4944302"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="AC16CM_03495"
     CDS             <1..1627
                     /locus_tag="AC16CM_03495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3138922.1"
                     /translation="EQLVQSVADPTGLGVLYLGPGHEPLAARDGLGNSLDPAEAYRDL
                     GLDEEDLEQAASSPQGGGRLEWSEGRSGYVAVWPLTASDTAVKGVLVYDVPRDELGDT
                     LTYLRYGILGAILTSILLAGAASVLLTHQVTRPLSETRDAAIRVASGDYSATVPVKSS
                     DELGEVARTFNYMAEEIEHYVGEIQEQKSRLEAVLQASPEAVVATDPEERVTMVNFAA
                     ARMLGIMASDRGRTLEEIRIPDGVLRCVREASTNGVAVREIEVGEKAYWAYAAQMDRG
                     EGASSNGTPGDGNAGIILAVRDITEHRSLEKAKTAFVSDFSHELRTPLTTIQSAVGLL
                     ERARERLDPLELRALELADQELGRIRGMVEELMTLAQMDSWQYQLEIGPANLSTVILT
                     AMESVEVKAERFGIRIYFEDAGEHRCACDVQKLYQVFINLLDNAIKYSDPGARVDIGV
                     EEDDTSLTVRIKDTGLGIPEEDLGQLFDRFYRVDKDRSRSSGGSGLGLAISKQIVEMH
                     GGSISVESELGAGSVFEVSLPKAFLSRSVSQAL"
     gene            1629..2819
                     /locus_tag="AC16CM_03500"
     CDS             1629..2819
                     /locus_tag="AC16CM_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3138923.1"
                     /translation="MARSGLLVALLAAVLLTGCTHIQNSYIPPLTPQTGGVDSGLGQG
                     TRALSSGPGYKGLPAWNRDGTRISFTVDGYVMDTSTGSGESGRLTTRDFVATDTEWTS
                     DETLAILGTTSPTGLEADNSLYRATPGGGSLALTRIAGSVLAMSPLPEEDDLLFALAT
                     GRHGSELAQTSDGGVTYKTYSDNHIDGHVSALSSSPGGDEALMSVHPRGDSRTSELRT
                     FDLRRGEGRQVARLEGDREILGTPQWMQQSIYFVAGKQTTNLDDDGSEPLFELYRVPA
                     DGGAPEPAPGVGEDFVASSIRVSPQGDRLAVIGRLNPKSPINLYVLDPATKKLEAVTA
                     NEDMEIKTDPGDLAWSPDGKNMAIVARVTPSTTPEVRAEPAQSILSDFYNLYEIPVSE
                     ETSR"
     gene            2816..3313
                     /locus_tag="AC16CM_03505"
     CDS             2816..3313
                     /locus_tag="AC16CM_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138924.1"
                     /translation="MRGVVVWLSGATLAFLLTAGAFLLLANIATIPDERTLSPKPPAA
                     ASQTLVGLELDPDQLASLRSSTDQNLDLTLRNEGDSPLTHVNVTLMVSSENTALSASR
                     YYQHSVARVPARGATNLRFGFDLSEPKQHVTGRPASEPAREILEFKATTPGGISTTKT
                     VILPP"
     gene            complement(3354..3965)
                     /locus_tag="AC16CM_03510"
     CDS             complement(3354..3965)
                     /locus_tag="AC16CM_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138925.1"
                     /translation="MKPTSFVSTVGEISAEEAVGVPDLDAVISYWTEDGYTAEEWFHE
                     LNSQWGTAGFVLRRGGEVQGFVVYALPEHLKGVVRYPLAIDDEAVVLAYAGGDPRTRR
                     RLLVRMLRDLRQRGFWKVQAIASDRGLSNHVSTSFLLESGWQPLHRAPYRMSYYTLFN
                     IDLKSAVEVGELARTLVGRVKLPGIQSPVPGTLARSTGEQSSR"
     gene            complement(3962..4390)
                     /locus_tag="AC16CM_03515"
     CDS             complement(3962..4390)
                     /locus_tag="AC16CM_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3138926.1"
                     /translation="MVDKQRLRTFVLGGAVGALVGVLLAPRSGRELRGSLANRAGEAR
                     ERGRESYFGAQEQMRDRLAEAREGSRRPRPDVADLGGSPSQEEEPEVSRPHLRDVSRD
                     VIAGAEEPADEERSEELRRKVKETRERLRGREGGEPGREP"
     gene            complement(4422..5405)
                     /locus_tag="AC16CM_03520"
     CDS             complement(4422..5405)
                     /locus_tag="AC16CM_03520"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HIR3138927.1"
                     /translation="MEEREITIVKGGRETPFSRGILAQTLAQAGARPELSHQIASEVR
                     AELLAEQLYTVEEEEVLARVRNKLVTKDAIVVGRLDKWRLLRESTEPIVVLIGGATGV
                     GTSTLAADVARRLNIQSVVGTDSIREVLRRAISPDLLPLLHKSSYEIKREDLRVPVEE
                     DEMVLYGFRQQASQVAVGVEAIVDRGLKEGTNLVVEGVHLVPEIILNRYRDHPNVCTL
                     VVYLSEEHVHRARFYIRALGTAMRRPAEEYISYFREIRQIHDYILQSAERAGVQAVEN
                     VSIENTSDAAVEIVANRVSGIAEEAGRRAPSMLLDAGTPRQGVPITRAKSG"
     gene            complement(5433..>5840)
                     /locus_tag="AC16CM_03525"
     CDS             complement(5433..>5840)
                     /locus_tag="AC16CM_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024937.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3138928.1"
                     /translation="VVEVVLEKVRALDPGFGYAMVLVRALAGAALFETMYQPGSLPGG
                     GAVPGLVIGGGIAALVAVLKVLSRPVSDGGSAGVSTTFLSAIEDVVAILGGIIGLFLP
                     YLPLVLVAFLLFFFNRIRRRRGRKYGGLRILGD"
BASE COUNT         1033 a         2002 c         1840 g          965 t
ORIGIN      
        1 cgagcagctg gttcagtcgg ttgctgaccc gacgggcctg ggggttctgt acctgggtcc
       61 cggtcatgaa cccctggctg cccgcgacgg gctcggaaac tctctggacc ccgcagaagc
      121 gtaccgggac ctcggcctcg acgaggagga tctcgaacag gccgcctctt cgccgcaggg
      181 tggtggcagg ctcgagtgga gcgagggacg ctcgggttac gttgcggtgt ggccgctcac
      241 ggcttcggac accgccgtca agggggtcct cgtctacgac gttccacgtg acgagctcgg
      301 ggacacgctc acctacctgc gctacggcat actcggggcc atactcacaa gcatcttgct
      361 ggccggggcg gcgagcgtgc tcctcaccca ccaggtcacg cgtccactct ccgagacccg
      421 cgatgcggcc atcagggtgg cgtcggggga ttactcggcc accgtgccgg tcaagagcag
      481 cgacgagctc ggcgaggtgg cccgcacgtt caactacatg gccgaagaga tcgagcacta
      541 cgtcggggag atacaggagc agaagagccg gctggaggcc gtccttcagg cctcgcccga
      601 ggctgtggtc gccacggacc ctgaggagcg ggtgacgatg gtcaacttcg cggcggccag
      661 aatgctcggc atcatggcct ccgacagggg acgcaccctg gaggagataa gaatccccga
      721 cggcgtcctg aggtgcgtgc gcgaggcctc cacaaacggg gtcgcggtgc gggagatcga
      781 agtcggagag aaggcttact gggcgtacgc ggcccagatg gacaggggcg aaggggcatc
      841 ctctaacggc acgccgggcg acgggaacgc cggcatcatc ctggccgtca gggacataac
      901 agagcaccgg tcgctcgaga aggccaagac ggctttcgta tcagacttct cgcacgagct
      961 gaggacccca ctcaccacca tccagagcgc ggtcggtctg ctggagcggg cgcgcgagcg
     1021 gctcgacccc ctggaactcc gggcgctaga gctggccgac caggagctcg ggcgcataag
     1081 gggaatggtc gaggagctca tgaccctcgc ccagatggat tcctggcagt accagctcga
     1141 aatcggcccg gccaacctga gcaccgtcat cctgaccgcc atggagtccg tggaggtcaa
     1201 ggccgagagg ttcggtatca ggatctactt cgaggacgcc ggcgagcacc ggtgcgcctg
     1261 cgacgtgcag aagctctacc aggtcttcat caacctgctc gacaacgcga tcaagtactc
     1321 cgaccctggc gccagggtcg atatcggggt ggaggaggac gacacctccc tgaccgtacg
     1381 catcaaggat accggcctcg gtatccccga ggaggacctc ggccagctct tcgaccgttt
     1441 ctacagggta gacaaggacc gctcccgctc gagcgggggt agcgggcttg ggctggctat
     1501 tagcaagcag atagtcgaga tgcacggggg gagtatctcc gtggagagcg agctcggcgc
     1561 gggttccgtc ttcgaggtca gcctgcccaa ggcctttctc tcccgctcgg tgagccaggc
     1621 cctctgagat ggcaaggtcg ggcctgcttg tagccctcct ggcggccgtg ctcctgacgg
     1681 gatgcaccca catccagaac tcctacatcc caccgctcac cccgcagacg ggaggggtgg
     1741 attcagggct gggtcaggga acacgggctc tgagttcggg acctggctac aagggcctgc
     1801 cggcctggaa ccgcgacggg accaggattt cgttcaccgt agacggttac gtgatggaca
     1861 cgtccacggg ctcgggggag tcggggcgct tgacaaccag ggatttcgtg gccacggaca
     1921 cggagtggac ctccgacgag accctggcga tcctcggcac cacctcccca accgggcttg
     1981 aggcggacaa ctccctgtac cgggcgaccc ccggtggggg ctcgctggcc ctgaccagga
     2041 tcgccgggag tgtactggcc atgagccccc tacccgagga ggacgatcta ctcttcgcgc
     2101 tcgcaaccgg ccgccacggg agcgagctcg cgcagacgag cgacggcggg gttacctaca
     2161 agacctactc agacaaccac atcgacggcc acgtctcggc cctttcctcc tcgcccggcg
     2221 gcgacgaggc cctcatgtct gtgcatcctc gcggggattc cagaacctcc gagctgcgca
     2281 ccttcgactt gcggcgcggg gaagggcggc aggtcgcgcg gctggaggga gaccgggaga
     2341 tcctcggcac cccccagtgg atgcagcaga gcatctactt cgtagccggc aagcagacca
     2401 ctaacctcga cgatgacggc tcggagccgc tcttcgagct gtatcgtgta ccggccgatg
     2461 gcggcgcccc agagccggcg cccggcgtag gcgaggattt cgtagcctcc agcatccgcg
     2521 tctcccccca gggcgaccgg ctggccgtga tcggccgcct caaccccaag tcgcctatca
     2581 acctctacgt gctcgacccc gcgacgaaga agctcgaggc cgtgacggcg aacgaggata
     2641 tggagataaa gaccgacccg ggcgacctgg catggtctcc cgacggcaag aacatggcca
     2701 tagtcgcccg cgtcaccccc tccacaacgc ccgaggtgcg cgcggaaccc gcacagagca
     2761 tactcagtga tttctacaac ctctacgaga taccagtctc cgaggagacc tcgcgatgag
     2821 gggcgtggtc gtctggctct cgggcgcaac cctggccttc ttgctcaccg ccggggcttt
     2881 cctgctgctg gcgaacatcg ctactattcc cgacgagaga accctgagcc cgaaaccgcc
     2941 cgccgccgca tcccaaaccc tcgtggggct tgaactggac ccggatcagc tcgcctccct
     3001 gagatccagc acagaccaga acctggatct tacgctcaga aacgagggcg actcacccct
     3061 gacccacgtg aacgtgaccc tgatggtctc ctccgagaac acggccctct ccgcctcccg
     3121 gtactatcag cattcggtcg cgagggtccc ggcacgcggt gccaccaacc tccgcttcgg
     3181 gttcgacctc tcggagccca agcagcacgt cacgggacgt cctgcctcgg agccggcgcg
     3241 cgagatcctc gagttcaaag cgaccacccc aggcggcatc tcgaccacga agacggtgat
     3301 cctgccgcct tgagagcggt cagctatcag cggtcagcta cccgctgacg gctctaacga
     3361 ctactctgtt cgccggtgga gcgggccagg gtacctggga ccggggattg gatgccgggc
     3421 agcttcacgc gcccgacgag cgtccgggcc agctctccca cctcgacggc gctctttagg
     3481 tcgatgttga acagcgtgta gtagctcatc ctgtaagggg cgcggtgcag gggttgccag
     3541 ccgctctcga gcaagaacga ggttgaaaca tggttggaca gaccgcggtc gctggcgatc
     3601 gcctgtacct tccagaagcc cctctgcctg aggtccctga gcatcctcac caggaggcgc
     3661 cggcgcgtcc gcgggtcccc ccccgcgtag gcaagcacca ccgcctcatc gtcgatagcg
     3721 agcgggtagc ggaccacgcc cttcaggtgt tccggcaacg cgtagaccac gaacccctgg
     3781 acctctcccc cccgcctgag gacgaacccg gcggtccccc actggctgtt cagctcgtgg
     3841 aaccattcct cggcggtata tccgtcctcg gtccagtagg agatgacggc gtcgaggtcc
     3901 gggaccccga ccgcctcttc ggccgatatc tcaccgacag ttgacacgaa ggaggtgggc
     3961 ttcatggctc cctgccaggt tcgccgccct cgcgacccct gagccgctcc ctggtctcct
     4021 ttaccttcct gcgaagctcc tcggacctct cctcgtcggc gggctcttcg gcgccagcga
     4081 tgacgtccct ggacacatcc ctgaggtgag gccggctcac ctccggttct tcctcctggg
     4141 aaggagaacc accgaggtcg gcgacgtccg gtcgcggcct gcgggagcct tcgcgggcct
     4201 cggccagacg gtccctcatc tgttcctggg caccgaaata gctctcgcgg cccctctcgc
     4261 gggcctcacc ggccctgttg gcgagcgagc cgcgcagctc cctgccgcta cggggggcca
     4321 gcagtacgcc caccagggct cccacggcgc cgccgagcac gaaggttctc agtcgctgct
     4381 tgtccaccac cgctatctcc ttgtgggggt ttcagaatat tctacccgct tttcgcgcgg
     4441 gtgatgggta ccccctgccg cggcgtgccg gcgtcgagga gcatcgacgg cgcccggcgg
     4501 ccggcctcct cggcgatgcc ggagaccctg ttggccacga tctccacggc cgcgtctgag
     4561 gtgttctcga tagagacgtt ctccaccgcc tgcacgccgg ccctctccgc gctctgcagt
     4621 atgtagtcgt ggatctggcg tatctcccgg aaataggaga tgtactcctc ggcggggcgg
     4681 cgcatcgccg ttccaagggc cctgatgtag aagcgtgccc ggtgtacgtg ctcctccgag
     4741 aggtacacga ccagcgtgca gacgttcggg tggtcgcggt agcggttcag aatgatctcg
     4801 ggcaccaggt gcaccccctc gaccaccagg ttggtccctt ccttgaggcc cctgtcgacg
     4861 atggcctcga ccccgactgc cacctgggag gcctgctgcc tgaacccgta gaggaccatc
     4921 tcgtcctcct cgaccggcac cctcaggtcc tcgcgcttga tctcgtagga gctcttgtga
     4981 agcaggggga gaagatccgg gcttatggcg cgcctgagca cctcccttat ggagtcggtg
     5041 ccaaccacgc tctggatgtt caggcgcctc gccacgtcgg cggcaagcgt gctcgtgccg
     5101 actccggtag cacctccaat gaggaccacg ataggctcgg tcgactcgcg caagaggcgc
     5161 cacttgtcca gcctgcccac cacgatcgcg tccttcgtga ccagcttgtt cctgaccctg
     5221 gcaaggacct cttcttcttc gacggtgtag agctgctcgg ccagaagctc ggcgcgaacc
     5281 tcgctggcta tctggtggga aagctcggga cgcgccccgg cctgggcaag agtctgggcc
     5341 aggatacccc gcgagaacgg cgtctccctg ccccccttga cgatcgtgat ctcccgctct
     5401 tccattgcac cctcctggag tgccgacgga ccctaatcgc cgaggatgcg gaggcccccg
     5461 tactttctcc cccgccgcct cctgatacgg ttgaagaaga atagcagaaa ggcgaccagc
     5521 acaagaggca gatatggcaa aaagagccct attatcccgc ccaggatcgc caccacgtcc
     5581 tcgatggcgc taagaaaggt ggtcgagacg ccagcggatc caccatctga gacgggacgt
     5641 gagagaacct tgagcacggc gacgagggcg gcgatacccc cgccgatcac gaggccgggc
     5701 actgcccctc cgccaggtag agaccctggc tggtacatgg tctcgaacag agccgcgccg
     5761 gccagagccc tgaccagaac catcgcgtag ccaaacccgg gatccagggc cctgaccttt
     5821 tccagcacga cctccacgac
//
LOCUS       DBNGVD010000121         7522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4579980, whole genome shotgun sequence.
ACCESSION   DBNGVD010000121 DBNGVD010000000
VERSION     DBNGVD010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4579980"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="AC16CM_03530"
     CDS             <1..1062
                     /locus_tag="AC16CM_03530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR3138929.1"
                     /translation="PDAGIDAVSFDTDSRTRPADLAARRVYGVARAADGARIAYKKLD
                     STLRGNVAVELAAALEASGRERAIVAPAFPAAGRATVGGTQLVHGVPVDETEMRNDPR
                     TPVREAHGPTLLAGVFPYVGTLGTRDVADSERVRLAVEENECVVADAEHDSDLEALVR
                     AVPDPARVLWAGSAGLALALANVYPGPCAGGAGEQRTPASPVLVVVGSLSGVAREQVR
                     RLVEEHGNVAVEAGREDAVAATRMALSGGACAVVHSPEERDPSGKGSIIETLAEVVSR
                     LSEEGLFGGLVMTGGSTAVGVARRLGATGIELRGEVEAGVPVGTLIGRNPYPVVTKAG
                     GFGGPDTLVGAVQALAQGR"
     gene            1071..2069
                     /gene="pdxA"
                     /locus_tag="AC16CM_03535"
     CDS             1071..2069
                     /gene="pdxA"
                     /locus_tag="AC16CM_03535"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3138930.1"
                     /translation="MTQPLIAVTMGDPAGIGPEILARTFAEEGFREGNRAVVVGDATM
                     LERAVKLLGLPLRVNSINEPEGAGFAPGTVDVLSETDLPEDLPFGKLDARAGDAAFRY
                     VERATRLAGEGRVAAVTTAPLNKEAMHLGGHQYPGHTEILAELTGTKDYAMMLVTDEL
                     KVIHVSTHVSLAEAIERVEPERELAVIRLAHGSLRKLGVERPKVAVAGLNPHAGENGL
                     FGTEDAERIAPAVAAAVEEGIDATGPHPPDTVMMRARTGAFDIVVVQYHDQGHIPIKL
                     MGFDTGVNVTVGLPFFRTSVDHGTAFDIAGTGEADPSSLRAAIDLARTLSGDEEGS"
     gene            2072..3322
                     /locus_tag="AC16CM_03540"
     CDS             2072..3322
                     /locus_tag="AC16CM_03540"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor-dependent isomerase,
                     group 22"
                     /protein_id="HIR3138931.1"
                     /translation="MEFPKVVKIRQRFPRLRVENVEEALREQLWRGEISSAIKPGMSV
                     ALTAGSRGIAQINEILRSMVGILKELGAEPFIVPAMGSHGGATAEGQVEILESLGVTE
                     EYCGAPIRSSMEVVQIGETGRGVPVYMDRIASEADGVVLVNRVKAHTDFRSSIESGLM
                     KMASIGLGKHEQALALHGFGVEGIRDFMVEVADEVLGSGHILFGVATVENAYDEPAII
                     EAIPAGQIHEREEELLAEYMGMMPALPVSDVDVLYVDALGKNYSGTGMDTNVIGRFRI
                     LGVDEPESPAVKYIVVGDVSEESHGNALGVGLADLTTQRLADRVEREAMNANVITSTF
                     VERAKVPMVLASDEEALRTAIRCNWGVPPDETRLVRIPNTLHLEYLYVSENMVDEALA
                     NAETEVVGDPEKLRFDREGYFEGF"
     gene            3462..4268
                     /locus_tag="AC16CM_03545"
     CDS             3462..4268
                     /locus_tag="AC16CM_03545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018832338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3138932.1"
                     /translation="MDFASFSGARVVDLSLTLSERLPGTWPGHMTYAHHNWNWFAEVG
                     GPTGTTRSSAPYQTNFLVIDEHCGTHFDAPTHFVPPPDSGLPLAGPLGDQTGDRVPLG
                     DLMGPAVVVDVRFLADTGEPGVSPFITADHVRAFEEEHGPLREGEVVLFRTGWDRYYV
                     EGDEGEKYMQGSLVTGEYPGWPAPAPETAVYLHERGIKTIGIDAPSIGSAHDGVPVHQ
                     EGLCRGMRYVEILTNLGELPVRGAFFIFMPLKIADSSGGPGRAMALVGGA"
     gene            4269..4658
                     /locus_tag="AC16CM_03550"
     CDS             4269..4658
                     /locus_tag="AC16CM_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3138933.1"
                     /translation="MPEHLNAPGLSDPPGYSHAVVASGGRLVTTAGAVPLDAGGNLVG
                     RGDYAAQTLQVLDNLGLALAAAGADGRDVLKTTVYVVTEDRSDLSDVWRVIQGSEVAQ
                     AASTLLGVSLLGYEGQLVEVEAIAVTG"
     gene            4787..6016
                     /locus_tag="AC16CM_03555"
     CDS             4787..6016
                     /locus_tag="AC16CM_03555"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3138934.1"
                     /translation="MQLFEELIEYPEAERLVLQSTPRLPVEEVPLEEALGLALARDLA
                     ATTDSPPFDNSAVDGFAIRSGDAEVGRVFHVVDEAPAGRPALGSVGEGEAIKIFTGGV
                     IPEGADCVVMVENTSGWGETFELKKVPAPGNNLRAAGEDTLRGDIILAGGTEIGSPEV
                     ALAATQGYGALPVYRRPKVVVLSTGTELVEPGTRGLEPGEIFDSNSYALVAAAREAGA
                     EASRLYAASDDVDTLRSAIGEALQTADVVVTSGGVSVGEKDLVKSTMLDLGVEQVFWG
                     VKFKPGKPLFYGTRGETRLFGLPGNPVSAMVCFDLFVRPALMRMTGRTDRNRPRVEVY
                     FERAIVNKFGRLHAMRVSLERTEKGWLATSVGGQGSGLVSSLTRADALALIGPASDGI
                     PPGEPIEAIVLRQDKLL"
     gene            6114..7157
                     /locus_tag="AC16CM_03560"
     CDS             6114..7157
                     /locus_tag="AC16CM_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3138935.1"
                     /translation="MFESNFEDNVFRRRLMKEVAPGVPVHLSFYSPSVKVQVMAPAEN
                     LPGANGRYIGDAVTIEFVPDDTAAVERVRELVWRWEDHNAFTAVRKVNHLRRSVCPES
                     VQGTLERVRGVDKRAAAKTAEVLEYEELREILGRGDFKTLARLPGMGEKRARAVVEFY
                     ENELGERRFLYAANRNDRFRGKPVITELDLAGDLKEQAWEHAIRARRLGDPDPLGPEE
                     PPGHTTVRNANLMHPLPMTPSLMGRGVYYPEQIELFADALKRVVDGEKLAGVEALRIQ
                     RGKLFHTTKMAGVGMKTRARVMASGLLDDEYTYESLIAIPGLTQTQARTIADAAKKRL
                     AALGPARTEVRVS"
     gene            7147..>7522
                     /locus_tag="AC16CM_03565"
     CDS             7147..>7522
                     /locus_tag="AC16CM_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR3138936.1"
                     /translation="MSPSLYGFVREHEPVSPEVVSTQFLSLANPNGDARALVEGLIGD
                     DPRFFWEDGSLKIAHAGSLPLDEAPYVVFDVETTGSAAGKGGAITEFGAMKLVRGEVV
                     DQFSTLVNPERPIDPFVVRLTGI"
BASE COUNT         1318 a         2364 c         2652 g         1188 t
ORIGIN      
        1 ccagacgctg gtatcgacgc cgtatcgttc gacaccgact cgcgcaccag gcctgcggac
       61 ctcgccgcca ggcgcgttta cggggtggcg cgcgcggccg acggggcgcg catcgcatac
      121 aagaagctgg actccaccct ccgcggcaac gtggccgtcg aacttgcggc ggccctcgag
      181 gcttcggggc gcgaacgggc catcgtggcg ccggcctttc cggccgccgg gcgcgctacg
      241 gtgggcggga cgcagctcgt acacggcgtt cccgtcgacg agacggagat gcgcaacgac
      301 ccgcgcaccc ccgttcggga ggcccacggc ccgaccctgc tggccggggt gttcccgtac
      361 gtgggcaccc tgggcaccag agacgtggct gactccgaac gggtacgtct ggcggtggag
      421 gagaacgagt gtgtggtcgc cgacgcggaa cacgattccg acctggaggc gctggtgcgc
      481 gccgtgcccg acccggcccg agtgctctgg gccggctcgg ctgggctagc gctcgccctc
      541 gcaaacgttt atccagggcc ctgcgcagga ggggcaggcg aacagaggac gccggccagt
      601 cccgtcctcg tggtcgtcgg tagcctcagc ggcgtcgcca gggagcaggt gcggcggctg
      661 gtcgaagagc acgggaacgt cgccgtcgaa gcggggcggg aagacgccgt cgcggcaact
      721 cggatggctc tttcgggcgg agcgtgcgcc gtcgtgcact cgcccgagga gcgggatccc
      781 tccggcaaag gatccattat cgagacgttg gccgaggtgg tctcccgact ctcggaagag
      841 gggctattcg gggggctcgt catgaccggt gggtctaccg ccgtcggggt agcgcggcgg
      901 ctaggggcga ccgggatcga gctccgcgga gaggtggagg cgggcgttcc cgttgggact
      961 ttgatcggcc ggaaccccta tcccgtcgta acgaaggccg gcggcttcgg cggaccggat
     1021 acactagtag gggcagttca ggcgctcgcg cagggcaggt aggagaacca atgacacaac
     1081 ccctcatagc cgtgacgatg ggagacccgg ctgggatcgg gccggagata ctggcaagga
     1141 ccttcgctga agagggcttt cgggagggaa accgggcggt cgtcgtcggg gacgcgacga
     1201 tgctcgaacg tgccgtaaag ctgctcggcc ttcccctgcg cgtgaatagt atcaatgagc
     1261 ccgagggggc ggggttcgcc cctggcaccg tggacgtgct ctccgagacc gacctgcccg
     1321 aggatctgcc cttcggcaag ctggacgcgc gggctggaga tgctgccttc cgctacgtcg
     1381 agcgggccac gcggttggcg ggggaggggc gcgtggcggc ggtaaccacc gcgccgctca
     1441 acaaggaagc catgcatctg ggcggccacc agtatcccgg gcacaccgag atcctggccg
     1501 aactgaccgg cacgaaagac tacgcgatga tgctcgtcac agacgagctg aaggtgatcc
     1561 acgtctccac ccacgtctcg ctcgcggaag ccatagagcg tgtcgagccg gagcgggagc
     1621 tggccgtaat ccgcctcgcc cacgggtcgc tgagaaagct aggcgtcgag agaccgaagg
     1681 tcgccgtcgc cggcctgaac ccgcacgccg gggagaacgg gctcttcggc acggaagacg
     1741 cggagcgcat agcgccggcc gtggccgcag ccgtagagga aggaatcgac gcgacggggc
     1801 cgcatccgcc ggataccgtg atgatgcggg ccaggacggg cgcgttcgac atcgtggtcg
     1861 tgcagtacca cgaccagggg cacataccca taaagctgat gggcttcgac accggcgtca
     1921 acgtcacggt gggcttgccc ttcttccgga ccagcgtgga tcatggcacc gccttcgaca
     1981 tcgccggtac gggggaggca gacccttcga gcctgcgcgc cgccatcgac ctggcccgca
     2041 cgctgtcggg ggatgaggaa gggagttaga gatggagttc ccgaaggtgg taaagatcag
     2101 gcagaggttt ccccgtctgc gcgtagagaa cgtggaggag gctctgcggg agcagttgtg
     2161 gcgtggggag atctcatccg ccatcaagcc gggcatgagc gtcgcactca ccgcgggaag
     2221 ccgcggcatc gcgcagataa acgagatcct gcgctccatg gtcgggatct tgaaggagct
     2281 gggggcggag ccgttcatag tgccggctat ggggagccac ggcggggcca cggccgaggg
     2341 acaggtcgag atcctggaat ccctgggcgt aacggaggaa tattgcggcg caccgatacg
     2401 ctcctcgatg gaggtggtcc agatcgggga aaccgggcgg ggggtgcccg tttacatgga
     2461 caggatcgcc tcggaggccg atggcgtggt gctggtcaac agggtcaagg cccacacgga
     2521 ttttcgctcc agcatagaga gcggcctgat gaagatggcc tccataggcc tgggtaaaca
     2581 cgagcaggcg ctcgccctgc acggcttcgg cgtcgagggc atccgagact tcatggtcga
     2641 ggtcgcggat gaggttctgg gctccgggca catcctgttc ggcgtggcga ccgtggagaa
     2701 cgcctacgac gagccggcca tcatagaggc gataccggcc gggcagatcc acgagcgtga
     2761 agaggagctt ctggccgagt acatgggcat gatgccggct ctgcccgtct cggacgtaga
     2821 cgtgctctac gtcgacgctc tgggcaagaa ctactctggg acggggatgg acaccaacgt
     2881 aataggccgc ttccgcatcc tcggtgtcga cgagccggag tcgcccgccg tcaagtacat
     2941 cgtcgtgggc gacgtcagcg aggaatcgca cggcaacgcc ctcggggtgg ggctcgccga
     3001 cctgaccaca cagcgtctcg ctgaccgggt cgagcgcgag gccatgaacg ccaacgtgat
     3061 cacgagcacg ttcgtggagc gggcaaaggt gcccatggtc ctggccagcg acgaggaggc
     3121 cctgcgtacc gccatccgct gcaactgggg cgtccccccc gatgaaacac gcctcgtccg
     3181 catcccgaac acgctgcacc tggagtacct ctacgtctcg gagaacatgg tcgacgaggc
     3241 gctcgccaac gccgaaaccg aggtggtcgg tgacccggag aagctgcgct tcgacaggga
     3301 aggatatttc gagggttttt gacgtagacg aatcgacctc ccgcgaccct tctcgaaccc
     3361 cgggtgaaca ttgcggcgaa cccgggaggg tcatacagta ggggaacgtc cagcgtgtgg
     3421 cgttcgggga ggttttcaga cgttttggga gaggagtagg cttggacttc gcatcgtttt
     3481 cgggggcgag ggtcgtggat ctttcgctga ccttgtcgga gcggttaccc ggaacctggc
     3541 cgggacacat gacctacgcc caccacaact ggaactggtt cgccgaggtc ggggggccga
     3601 cgggcacgac gaggagcagc gccccctatc agaccaactt cctcgtcatc gacgagcact
     3661 gcggcaccca cttcgacgcc ccgacccact tcgtcccgcc ccccgattct ggtctgcccc
     3721 tggccgggcc tctcggggat cagacgggcg acagggtccc actcggggac ctgatgggcc
     3781 cggcggtcgt ggtcgatgtg cgcttcctgg ccgacacggg cgagccaggc gtgagcccct
     3841 ttatcaccgc cgaccacgtc cgcgcgttcg aggaggagca cgggccgctg cgtgagggcg
     3901 aggtcgtgct cttcaggacg gggtgggacc gttactacgt cgagggagac gagggcgaga
     3961 agtacatgca gggctcgctc gtcaccggcg agtaccccgg ctggccagcc cctgcccccg
     4021 agaccgccgt ctacctgcac gagcggggca tcaagaccat cggtatcgac gccccgagca
     4081 tcggcagcgc ccacgacggc gtccccgtcc accaggaagg cctctgccgc gggatgcgct
     4141 acgtggagat cctgaccaac ctcggcgagc tgccggtgcg cggcgcgttt ttcatcttca
     4201 tgccgctcaa gattgccgat tcctccggtg gaccgggccg ggctatggcg ctggtgggag
     4261 gggcctgagt gcccgaacac ctcaacgcgc cgggactgag cgatcccccg ggctactcgc
     4321 atgcggtggt cgcctcggga ggacgactcg tcacgacggc cggggccgtc cccctcgacg
     4381 ccggcggcaa cctcgtgggc cgtggagact acgccgcgca gacgctgcag gtcctcgata
     4441 acctcgggct ggccctcgcg gcggcaggcg ccgacggccg ggacgtgctc aagaccaccg
     4501 tttacgtggt cacagaggac agatccgacc tctcggacgt ctggcgtgtc atccaggggt
     4561 cggaggtggc gcaggccgct agcacgctcc tcggcgtctc gttgctggga tacgaggggc
     4621 agctcgtgga ggtcgaggcc atagcagtaa ccgggtagcg ctaatgcagg accgtcatcg
     4681 ccgtggacca gaaaaccgga gtcgaaagcc gatagctgac ggctgacagc acaagaaatg
     4741 tggtagcgtc tctgcagcaa cgagataccc aaacagaggt gccagagtgc aacttttcga
     4801 ggagctgatc gagtaccccg aggctgagcg gctggtgctg cagagcacgc cgcggttgcc
     4861 cgttgaggag gttcccctgg aggaggctct tggcctcgcg ctcgcgcggg acctcgcggc
     4921 aacgaccgac tcgccgccgt tcgacaactc ggccgtggac ggcttcgcga tccgcagcgg
     4981 ggacgcggag gtcggtcgtg ttttccatgt ggtggacgag gcgccggcgg gacgtccggc
     5041 actcgggagc gtcggtgaag gtgaggcgat caagatcttc accgggggtg tgatccccga
     5101 gggcgccgac tgcgtggtga tggtcgagaa cacgtcgggc tggggcgaga cgttcgagct
     5161 caagaaggta ccggcccccg gcaacaacct ccgcgccgct ggcgaagata cccttcgggg
     5221 agacatcatc ctggccgggg ggaccgagat cggctctccc gaagtggcgc tcgcggcgac
     5281 gcagggttac ggggccctcc ctgtctaccg caggccgaag gtggtcgttc tctcgacggg
     5341 gaccgagctc gtcgagcccg gtacgcgcgg gttggaacct ggtgagatct tcgattccaa
     5401 ctcctacgct ctcgtcgctg cggcccgtga ggcgggtgcc gaggccagcc gcctctacgc
     5461 ggcctccgac gacgtggata ccctgcgttc cgccataggg gaggccctgc agacggccga
     5521 cgtggtcgtg acgagcggcg gggtgtcggt tggggagaag gacctcgtca agagcacgat
     5581 gctcgatctt ggcgtggagc aggtcttctg gggcgtcaag ttcaaaccgg gcaagcccct
     5641 cttctacggc acgcgtggcg agacccgcct cttcggcctc cccggcaacc ccgtctccgc
     5701 gatggtctgc ttcgacctct tcgtccgtcc agcccttatg cgcatgacgg gccgcacgga
     5761 caggaacaga ccccgtgtcg aggtctactt cgagcgtgcg atcgtcaaca agttcggcag
     5821 gctgcacgcc atgagggtga gcctcgaacg cacggagaaa ggttggctcg ccacgtccgt
     5881 tggcggccag ggctccggcc tcgtcagctc gctaaccagg gccgacgccc tcgcccttat
     5941 cggacccgcc tccgacggca tccccccggg agagcccata gaagccatcg tgctgcggca
     6001 ggacaagctg ctgtaaggcg acagtttcag ggttttttcc gatgcctgat gcctgtaacc
     6061 tgaaacctca tctccacgag tcgaggagct tctagtgtta gaatccaggt gcgatgttcg
     6121 agtccaattt cgaagataac gtctttcgca ggcggctgat gaaagaggtt gcgccggggg
     6181 ttccggtcca cctctcgttc tactcgccct cggtcaaggt acaggtgatg gcccctgcgg
     6241 agaacctgcc cggtgccaac gggcgctaca tcggggatgc ggtcaccatc gagttcgtcc
     6301 ccgacgatac ggcggctgtc gagcgggtcc gcgagctcgt gtggcgctgg gaggatcaca
     6361 acgccttcac ggcggtacgc aaggtcaacc acctcaggcg cagcgtctgc ccggagagcg
     6421 tgcagggcac gctcgaacgg gtccgtggcg tggacaagcg ggcagcggcc aagacggccg
     6481 aagtgctgga gtacgaggag ttgcgcgaga tcctcggccg gggagacttc aagaccctgg
     6541 cacggctgcc tggcatgggg gagaagcgtg ccagggcagt cgtggaattc tacgagaacg
     6601 agctgggcga gaggcgcttt ctgtacgctg caaaccgcaa cgaccggttc aggggcaagc
     6661 cggtaatcac ggagctggac ctcgcgggag acctgaaaga gcaggcttgg gagcacgcta
     6721 tcagggcgcg caggctcggc gacccggacc cgctcggccc ggaagagccc ccgggacaca
     6781 cgaccgtccg caacgcgaac ctcatgcacc cgctcccgat gacgccctcg ctcatgggcc
     6841 ggggcgtgta ctacccggag cagatagagc ttttcgccga tgccttgaag cgggttgtcg
     6901 atggggagaa gctcgccggc gtcgaggcgc tgcgcatcca gcgcggcaag ctcttccaca
     6961 ccaccaagat ggccggcgtt gggatgaaga cgcgggcgag ggtgatggca agcgggctgc
     7021 tcgacgacga gtacacctac gagagcctca tcgccatccc tggcctcacg cagacgcagg
     7081 ctaggaccat cgcagacgcc gcgaagaaga ggctggccgc gctcgggccg gcgcggacgg
     7141 aggtccgtgt ctcctagcct ctatgggttc gtccgagagc acgagcccgt tagcccggag
     7201 gtcgtctcca cacaatttct ctctcttgcg aaccccaacg gcgacgcccg cgccctcgtg
     7261 gagggcctga tcggggacga ccccaggttc ttttgggagg acggtagcct gaagatcgcc
     7321 cacgccggct cgctcccgct ggacgaggcc ccttacgtcg tcttcgacgt ggagaccacc
     7381 ggttccgctg ccgggaaggg tggcgccata accgagttcg gggccatgaa gctcgtgcgt
     7441 ggggaggtgg tggaccagtt ctccacgctc gtgaacccgg agaggcccat agaccccttc
     7501 gtggtgcgcc tgaccggcat aa
//
LOCUS       DBNGVD010000122         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3486853, whole genome shotgun sequence.
ACCESSION   DBNGVD010000122 DBNGVD010000000
VERSION     DBNGVD010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3486853"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16CM_03570"
     CDS             <1..971
                     /locus_tag="AC16CM_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIR3138937.1"
                     /translation="MELGGLHHMTAVTTDAAENVAFYTDVLGLRLVKKTVNQDDVSAY
                     HLFYGDEVGNPGTEITFFDWPQLRIAPNSPGAGEVSTTELRVPDRAALEWWVGRFQEL
                     GVPHGEIEENGRAHLDFTDHEGQRLRLSADEGQGVPGGVPWQESTVPAEVGILGLGAV
                     DLTVRELDPTALVLTEILGFRKVDESVHAGGQTVTFEVGEGGPGATVRVVERPDASWT
                     RLGRGGVHHIAFRTPDDEEHAAWRERLRRAGLGVTPQIDRFYFRSIYFREPGGVLFEI
                     ATDGPGFATDEDAAHLGERLSLPPFLEDRREAIEAGLTPISTAARR"
     gene            1214..>2709
                     /locus_tag="AC16CM_03575"
     CDS             1214..>2709
                     /locus_tag="AC16CM_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3138938.1"
                     /translation="MANLECRTATSRPNKWVALTVVCLVVFVICINTTAINTAVATIA
                     DDLSLSTSTLSWALDVYMLAAAALVVLGGQVGDILGRTPITLVGIGLFAVGSVVVATS
                     FSGAQLIGGRALQGAGGAVLMPATMAIINDTFSREESGTALGVWGAVSMLAFAFGPLY
                     GGILTEAISWRVIFWTDVALLLIAAALLLSSLRGLPGGRRGIAVDIVGAILLGSGSFL
                     VVLALQQGQGWGWASTRFLVTLCIGLSSLVAFLVVELRRHNPLLNLALFRRRRFVGGC
                     LGTFANTVGLIGLLYFFNLYAQSSVLFDLSPLHASLVLLPYTVSMFLFAYPSGRIADR
                     IGYRIPVVVGLLVMTAGFVMLSLVGVSTTEADLYLPIVLCGLGVGLTYSTTSAAGMSS
                     VPAGEAGQGAGVINMARFLGAVFVIAAGTILYVGQGVATLDEHLDRAKAGEVEKTKLD
                     RVLSGSPGALGSAAKELEPRTREAFVAGARQGIAGGFSDVMWLIAAVGA"
BASE COUNT          426 a          836 c          948 g          499 t
ORIGIN      
        1 agatggaact tggcggattg caccacatga cggcggtaac cacagacgcg gcggagaacg
       61 tggcgttcta cacggacgtg ctcggcctcc ggctcgtaaa gaaaaccgtc aaccaggacg
      121 acgtgtcagc ctaccaccta ttttatggag acgaggtagg gaacccgggc accgagatca
      181 ccttcttcga ctggccccaa ttgcgcatcg cccccaacag ccctggcgcg ggtgaggtct
      241 cgaccaccga gttgcgcgtt cccgaccggg cggcgctcga gtggtgggtt ggccggtttc
      301 aggagctcgg cgtgccacac ggcgagatcg aggagaacgg ccgcgcccat ctggacttta
      361 ccgatcatga ggggcagcgc ctgcggctct ccgcggacga gggccagggc gttcccggcg
      421 gggttccgtg gcaggagagc accgttccgg cggaggttgg gatcctgggc ctcggggcgg
      481 tagatctgac ggttcgcgag ctcgacccca cggccctggt actgaccgag atactcggct
      541 tccggaaggt ggacgagagc gttcatgccg gtggccagac cgtaacgttc gaggtcggcg
      601 agggtggccc gggggccacg gttcgggtgg tcgagcggcc cgatgcctcc tggacgcgtc
      661 tcggacgcgg aggcgtgcac cacatagcct tcagaacgcc ggacgacgag gagcacgccg
      721 cctggaggga gaggctccgc cgcgccggtc tcggggtgac gccgcagatt gaccgcttct
      781 acttccgctc catctacttc cgcgagccgg gcggggtgct gttcgagatc gccaccgacg
      841 ggccaggatt cgccaccgac gaagacgccg cgcacctcgg cgagaggctc tctctacccc
      901 ccttcctcga agaccgcagg gaggccatag aggccggcct gacccccatc tccacggcag
      961 cgagacgtta agagaagccg gggcgtccag gacgcggggg ctgcggtggg acccgggcga
     1021 ggacgtccgg gacagacggt tctgggccaa gcgggtgcgc cagcccttac ctgcgaaccg
     1081 gagaacagct accataagta tcgtcagagg acggtggttg ccgtagaagg gcgaccgcgt
     1141 agtcccacgg tccggcatga ccccgaagtt cgggagggcg ggaggccgta gaggatggcc
     1201 gatggagagc ggcgtggcga acctagagtg tagaacagcc acttctaggc cgaacaagtg
     1261 ggtcgccctg accgtcgttt gcctggtcgt cttcgtaatc tgcataaaca ccacggcgat
     1321 caacaccgcg gtcgccacga tagcagacga cctctccctc tcgacctcca ccctgagctg
     1381 ggcgctggac gtttacatgc tggcggcggc ggcgctcgtg gtgctcggcg gccaggtagg
     1441 tgacatcctc ggtaggacac cgatcacgct agtaggcatc ggtctcttcg cggtcgggtc
     1501 tgtggtggtc gccacctcgt tttcgggtgc acagctgatc gggggccggg ccttgcaagg
     1561 cgcgggtggc gccgtgctta tgccggccac catggcgatc atcaacgaca ccttctccag
     1621 ggaagagagc gggaccgctt taggggtgtg gggagcggtc tccatgctcg ccttcgcctt
     1681 cgggccactg tacgggggga tcctcacaga ggccatctcc tggcgggtga tcttctggac
     1741 cgacgtggcc ctcctgctga tcgccgcggc gctcttgctc tcctcgttgc gtggcctgcc
     1801 gggcggccga agggggatcg cggtcgatat cgttggggcg atcctgctcg gctcgggaag
     1861 tttcctggtg gtgctggcgc ttcagcaggg acagggttgg gggtgggcat caaccaggtt
     1921 tctggtcacg ctgtgcatcg gtctctcgtc gctggtggct ttcctggtgg tcgagttgcg
     1981 ccggcacaat cccctgctaa acctcgctct gtttcggagg cgtcgcttcg tcggtggctg
     2041 tctcggcacg ttcgccaaca ccgttggcct gatcgggctg ctctacttct tcaacctgta
     2101 cgcccagtcc tccgtgctct tcgacctctc gccgttgcac gccagcctgg tgttgttacc
     2161 ctacacggtt tcgatgttcc tcttcgccta cccgtcgggc cggatcgcgg accggatagg
     2221 ctaccggatt cctgtggtcg tcggcctgct ggtaatgaca gcgggcttcg tgatgctctc
     2281 gctagtaggg gtcagcacca cggaggccga cctctatttg cccatagtgc tgtgtggcct
     2341 gggggtaggg ctgacctact cgaccacctc cgcggcaggc atgtcctcag tcccagccgg
     2401 agaggccgga cagggcgctg gggtgatcaa catggcccgc tttctgggcg cggtgttcgt
     2461 catcgcggcc ggaacgatcc tgtacgtggg ccagggggtt gcgacgctcg acgaacacct
     2521 ggacagggcg aaggcgggag aggtcgagaa gacgaagctc gaccgggtct tgagcggatc
     2581 tcctggtgcg cttggaagcg cggcaaagga gctggagccc cgtaccaggg aggccttcgt
     2641 cgccggtgct cgccagggca tcgccggcgg gttctcggac gtaatgtggc tcatcgcggc
     2701 cgtgggggc
//
LOCUS       DBNGVD010000123         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3487154, whole genome shotgun sequence.
ACCESSION   DBNGVD010000123 DBNGVD010000000
VERSION     DBNGVD010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3487154"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC16CM_03580"
     CDS             complement(<1..340)
                     /locus_tag="AC16CM_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138939.1"
                     /translation="MGFEESALERLRRYRGEADRSIGAAHEAEAMARRFADRLFAGLE
                     YTAVLGRQAGFEVQTTYAGGALDLRTIAGGGAEAGVSFGVVPGAAAETDENLMHEEFS
                     HYSLDPSGYSG"
     gene            complement(345..1100)
                     /locus_tag="AC16CM_03585"
     CDS             complement(345..1100)
                     /locus_tag="AC16CM_03585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3138940.1"
                     /translation="MAIVTGASSGIGEATARELASRGASVVLASRAVESLEGLRREIS
                     ASGGVALVVETDVSDRGSVEAMVRRAVGEFGSLDVLVNNAGLGLSGRVSEVRAEDLRH
                     VFAVNVLGPLNCIQAALPHMGDGGRIINVSSVVGKRAIPKVGAYCATKSALDALSDAL
                     RVEMGGKITVTSVFPGTTRTNFRENSRRTKSEKRGWRPKGVTPDRVAVRIANAAEKGS
                     RDVYVTLPDRLFVAGVTLFPGLADRMLRSWAKD"
     gene            complement(1177..1353)
                     /locus_tag="AC16CM_03590"
     CDS             complement(1177..1353)
                     /locus_tag="AC16CM_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138941.1"
                     /translation="MVSKMESLRWLESLKRSQRLTAREEGRKEEILGIFYELYDISLR
                     YEDPPVFPPWRPSW"
     gene            complement(1733..>2280)
                     /locus_tag="AC16CM_03595"
     CDS             complement(1733..>2280)
                     /locus_tag="AC16CM_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3138942.1"
                     /translation="VAAGLSDAFENGFRVHIGRSAPEHDDVPACAILDTGGSETLAEG
                     IKRIRGVSPDIPILVFGLRVDLSLARAALRHGARGFIHAGMDLDQVARATEVAARGEL
                     VVPRTILEQLVTSEDPADLDALTTRQREILELVAKGYSNARIAQELFLSESTVKQHLR
                     ATYKILGVSDRKEAAKLINGL"
BASE COUNT          370 a          797 c          713 g          400 t
ORIGIN      
        1 gtcctgagta gcccgatggg tcgagactgt agtggctaaa ctcctcgtgc atgaggttct
       61 cgtctgtctc ggcggcggct ccgggcacga ccccgaagga gacaccggcc tctgcccccc
      121 ctcccgctat ggtccgcagg tcgagcgcgc cgccggcgta ggtggtctgt acctcgaacc
      181 ctgcctgcct gccgagcacc gccgtgtact ccagcccggc aaagaggcgg tcggcgaacc
      241 ggcgggccat cgcctcagcc tcgtgagcgg ccccgataga gcggtcggcc tcgccacggt
      301 agcgacgcag gcgctcgagg gcgctctcct cgaaacccac ccgcctagtc cttcgcccac
      361 gaacgcagca tgcggtcggc gaggccggga aagagcgtga cgccggcgac gaagagccgg
      421 tccgggagcg tcacgtacac gtcgcgggag cccttctcgg cggcgttggc tatcctcacc
      481 gcgaccctgt ccggcgttac gcccttcggg cgccagccgc gcttctcgct cttcgtgcgc
      541 cgtgaattct cccgaaagtt ggtgcgggtc gtgccaggaa agacgctggt gacggtgatc
      601 ttcccgccca tctccaccct gagggcgtcg gagagcgcgt ccagggcaga tttggtcgcg
      661 cagtaggcgc cgaccttcgg aatggcgcgc tttccaacca cggaggagac gttgatgatg
      721 cgcccgccat cccccatgtg gggcagggcg gcctggatgc agttcagcgg gccaaggacg
      781 ttgaccgcga acacgtgcct caaatcttcg gccctgacct cggagaccct gccagacagc
      841 ccgagcccgg cattgttcac cagcacgtca agcgacccga actcccccac ggcccgcctc
      901 accatagcct cgacggagcc cctgtctgag acgtcggtct ccacgacgag cgccacgccg
      961 ccagatgcgg agatctcccg tcgcaggccc tcgagactct ccacggccct cgaggcgagc
     1021 acgaccgagg ccccgcgcga cgcgagttcc ctggccgtcg cctcgccgat accgctagaa
     1081 gcaccggtaa cgatagccac cctaccatcc aggctctgtc tcacgggaac gattacgtgg
     1141 ggcggacccg gcgcgccgaa cccggtagcc tgccgctcac caggaaggac gccagggcgg
     1201 gaagacggga gggtcctcgt agcgcaacga gatatcgtag agttcgtaga atatgcccag
     1261 gatctcctct ttgcgaccct cttcccgggc ggtcagccgc tgggatctct tcaaactctc
     1321 cagccagcgc aggctttcca tcttgctgac cactatcttc ctgccttccg tacctggcga
     1381 gccatctcta ccctcactcg atcgggggca cgtatagtag cacgctccgg ctcgggcttg
     1441 cgccctcgca gcacgctgcg gccctacggg cctccgctag tcagcacgcc ggctccgctc
     1501 cgctacaccg gcttgtcagc acgctcgggc taacgccctc gcttgtcggc aacgcttcgg
     1561 cttcacctcg ctttcggccg gtcgtctctc tccgtgacta caccttaggc cacccacctt
     1621 ctccaggcat cgatacatcc cgggagaagg gtgcaggccg caacgggatc tagctgaaag
     1681 cgagccaggc gaagccggac gaagcgtgct ggcaagccgc tttgcggctt gcctacagcc
     1741 cgttgatgag ttttgcggcc tctttacggt cactgacgcc gaggatcttg taggtggcgc
     1801 gcaggtgttg tttgacggtt gattcggaga ggaagagttc ctgggctatc cgggcgttgc
     1861 tgtaaccttt ggcgacgagc tctagtatct cgcgctggcg ggtggtcagc gcgtcgaggt
     1921 cggcggggtc ttcgctggtt acgagctgct cgaggatggt tcttggaacg actagctcgc
     1981 ccctcgcggc cacctccgtg gcgcgggcta cctggtccag atccatgcca gcgtggatga
     2041 agcccctggc cccgtgccgc agggcagcac gggcgagcga cagatccacg cgcagaccga
     2101 agaccaatat ggggatgtcc gggctgaccc cccggatgcg tttgatgccc tccgcgaggg
     2161 tctcggaccc gcccgtgtcc aggatggcac aggcaggaac gtcgtcgtgc tcgggggccg
     2221 accttccgat atgcaccctg aacccgttct cgaacgcgtc gctcaagccg gccgccacta
//
LOCUS       DBNGVD010000124         4595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1847218, whole genome shotgun sequence.
ACCESSION   DBNGVD010000124 DBNGVD010000000
VERSION     DBNGVD010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1847218"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..721)
                     /locus_tag="AC16CM_03600"
     CDS             complement(425..721)
                     /locus_tag="AC16CM_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR3138943.1"
                     /translation="MESADDRLDNPYTYLEGYKVSDATGEDVGEVDGTVYDAPSDVLK
                     YLVVGGRTIPADRIEVHVENNHVSVPYSAQTVRSAPRIEEPSGAFDDLVHEHFA"
     gene            773..1753
                     /locus_tag="AC16CM_03605"
     CDS             773..1753
                     /locus_tag="AC16CM_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138944.1"
                     /translation="MGGSTDLGRNARSLKAFSFLLLALVVASLVLVHADSYEPAVSYI
                     LLYLVASVGIVTAAVVVPLLPAEQFSAAMCGSIYALYAALIGMMVFFSGGVSSELYMM
                     FFPLLLGSALHGSWRVMLAVLVAVLFCYALAMLPGLLEQGTDQDFASLVFYRLAAVGF
                     VGLFLLYMAGAVLTGGLAEGQATDSLELLEKVEEELAARKGVPVGVILVDPGADLQVM
                     DALLERVRSRIGEPVPLGEDNLFGVVVSGLDDGGVESAARRALAAASSLGAQDTRAGA
                     AIYPRDARSAEDLLISAGRALEAAFEVESSSAIVIAGVHAGSSPNAARPV"
     gene            2032..2781
                     /locus_tag="AC16CM_03610"
     CDS             2032..2781
                     /locus_tag="AC16CM_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3138945.1"
                     /translation="MGRFTRAIRGFFGRFLSGVEQRNPEILLENAVQDELDNLKKLQV
                     AAARTMAYEKMLSNDAVTKQKTLAVKERQARELTSRGQREAAIAVVQQKQEAEASLAD
                     IEARLEEARSNSQELEQAFRDQERRYNDVVRERAALMEEHQRSRALRTANEARANISL
                     SDSSRDLDKARQSIRQASYEAQAVGELGTSDTERQIAAAEIDIKRLDAERELEMMEIE
                     MGLREPEKIEASPVEEAPEPQVEETEENPRG"
     gene            2774..3577
                     /locus_tag="AC16CM_03615"
     CDS             2774..3577
                     /locus_tag="AC16CM_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138946.1"
                     /translation="MGDLVVRKQNLSRRLMVRVAPFASFAGLGAFAAVEGSFTLAGLL
                     GIGALVTGRRLLDRRENARRELLSRARQNRAELGRVAREDRTAASQMKRLVALQDGLL
                     ESWDLLPAECKPLLGDDIFTIMGEIEVAARLARRRAALRRHLQSVNQRSIARRIKGLE
                     KDLAKLEPDSPMRAPFESALEGRRGELAGYPEVLDGISMINAQLESAESLLGNLRMEL
                     LALDDSLILGPQDPGLARLKERIAYFRHGLDEVTRRVETLPATERMSAR"
     gene            3574..4278
                     /locus_tag="AC16CM_03620"
     CDS             3574..4278
                     /locus_tag="AC16CM_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138947.1"
                     /translation="MNDDRNTARQSFFSRVFAGTTEGEGTAFYSPEEVGTSTPGIGEQ
                     GATAHGFTVERAAEVIKNLPPEVPRLSAVRIVRGTLEAAGIDIAELESSTRARESRLN
                     SEIDLSESRIQQLKDETEEVIRNLEDEIRKAREARNFGVSEEERKIQSAETGLDNIDL
                     VREFFGLPQEEGEPPVAAAAVDDTADHTADTGGEETQILNGVEEEEGDETQVLRRPGP
                     LSDSEDYWEAEDKKEP"
     gene            4275..>4595
                     /locus_tag="AC16CM_03625"
     CDS             4275..>4595
                     /locus_tag="AC16CM_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138948.1"
                     /translation="MSIPRRLGRLAQGFVSSLQTDDRFRERLVRGRERSETIRDAFEA
                     AWRGASEEWRRAEERRTAEEAARQNGASTRERNSGWRTAPPFIPLSYPANVLSAYNRL
                     GLAPG"
BASE COUNT          819 a         1434 c         1603 g          739 t
ORIGIN      
        1 tcctgggact cacccgccgt caaggacgtc gtgaactgcg gccccggcac ggacaaggtc
       61 tacgccgacg aggccgacgt catcaaagac aactgcgagc gggtgagggt gcagtaggag
      121 cgcgctccag cctagcggct gtcgcttgtc agcacgttac ggcccttcgg gcctccactt
      181 tcagcacgct cccttcggtc gcttgtcagc agcgcttcgc caggcttcgc atggctcgct
      241 gtcagccaga tcccgctgcg gcctgtaccc taccgctctg aaggttcgat gccgaggaca
      301 ggtgggctgt cgaagtcacc gttacggaga gagacgactg gccgaaagcg aggcgaagcc
      361 gatgcgttgc cgacaagcga gacccgctag gctggagcgt gctgacaagg cgcgaagcgg
      421 cgtgctacgc gaagtgctcg tggacgaggt cgtcgaaggc cccggagggt tcctctattc
      481 ttggtgccga tcgtacggtc tgggcgctgt aggggaccga gacgtggttg ttctcgacgt
      541 gcacctcgat ccggtcggcg gggatggtac gtccaccgac cacgaggtac ttcagcacgt
      601 cgctcggggc atcgtagacg gtgccatcta cctcaccgac gtcttcccct gtggcatcgc
      661 tgaccttgta gccctccagg tacgtatacg gattgtccag gcggtcgtcc gcgctttcca
      721 tcgttccacc tccggtggcg ggagcgagtg tacctgcgta tgatacttcg gcgtgggcgg
      781 tagtacagat ctcggtcgaa atgcccggag cctgaaagcg ttttcgttcc tgctcctggc
      841 cctcgtggtg gcctcgctgg tgctggtgca cgcagattcc tacgagcccg ccgtaagcta
      901 catcctgctc tacctcgtcg cttccgtcgg gatcgtcacg gcggccgtcg tggtgccact
      961 tcttcccgcg gagcagttct cagccgcgat gtgcggctcg atctacgccc tgtacgcggc
     1021 cctgatcggc atgatggtct tcttctccgg gggcgtctcc agcgagctct acatgatgtt
     1081 tttcccgctg cttctcggct cggccctgca cgggtcgtgg cgcgtgatgc tcgcggtgct
     1141 cgtggccgtc ctgttctgct acgccctggc gatgctgcca gggctgctgg agcagggaac
     1201 ggaccaggat ttcgcctcgc tcgtcttcta caggctcgcc gcagtgggct ttgtgggcct
     1261 cttcctcctc tacatggccg gggccgtgct gaccggcggc ctcgcggagg gccaggcgac
     1321 ggacagcctg gagttgctcg agaaggtcga ggaagagctc gcggcgcgca agggtgtgcc
     1381 cgtgggtgtg atcctggtcg accccggcgc cgacctgcag gtcatggatg ccctcctgga
     1441 aagggtgcgt tcgcgtatcg gtgagcccgt gccccttgga gaggacaatc tctttggcgt
     1501 ggtggtaagc gggctagacg atggtggggt ggagagcgcg gcgcgacgcg cccttgccgc
     1561 cgccagctct ctcggcgccc aagacaccag ggcgggtgcg gccatctacc cccgcgacgc
     1621 ccgctccgcc gaagacctcc tgatctccgc cggccgcgcc ctcgaagccg ccttcgaggt
     1681 cgaatcttcc agcgctatcg ttatcgcggg cgtgcatgcc ggcagttcgc cgaacgccgc
     1741 acgccccgtg tagttagcac gcttcgcccg gtgggggctt gctttcggcc agatcccgct
     1801 gcggcctgca ccttccgcac agggtgtatc gctgcgcaga aaggccccac acctggcttg
     1861 ccgttacgga tcgagacgac cggccgacag ggcgccgcca ggcgccgtgc tgaccagcgg
     1921 aggcgttagc cggagcgcgc tctgaaaccc acgccggggg ttacgcgtac cgatgatata
     1981 atttgacagg tactctgcag gcacggctac agccgggggt gggcggcaca gatgggacga
     2041 tttacgaggg ccattcgcgg tttcttcggg aggtttctct ccggggtcga gcagcgcaac
     2101 ccggagatac tgttggagaa cgccgtccag gatgagctgg acaacctcaa gaagcttcag
     2161 gtcgcggcgg cgcgcacgat ggcctacgag aagatgctct ccaacgacgc ggtgacgaag
     2221 cagaagacgc tggccgtaaa ggagcgtcag gcccgcgagc tcaccagcag gggccagcgg
     2281 gaggcggcca tcgcggtggt ccagcagaag caggaggcgg aggcgagcct cgcggatatc
     2341 gaggcgcggc tcgaggaggc gcgcagcaac tcgcaggagc tggagcaggc cttccgagac
     2401 caggaacgcc gctacaacga cgtggtgcgg gagagggccg ctctcatgga ggagcaccag
     2461 cgctcgcgcg ccctccggac ggccaacgag gcccgcgcca acatctccct ctccgactcc
     2521 tcccgcgacc tggacaaggc gcggcagtcc atcaggcagg cctcctacga ggcccaggcc
     2581 gtcggcgagc ttggcaccag cgacacggag cgccagatcg cggcggccga gatcgacatc
     2641 aagcggctgg atgccgagcg ggagctggag atgatggaga tcgagatggg ccttcgcgag
     2701 cccgagaaga tcgaagcctc cccggtcgag gaagcaccag agccacaggt cgaagagacg
     2761 gaagagaatc cccgtgggtg atctggtcgt aagaaagcag aacctctcac gacgcctgat
     2821 ggtgagggtt gccccgttcg cgtcgttcgc cggcctgggc gccttcgcgg ccgtggaggg
     2881 aagtttcacc ctcgccggac tgctcggcat cggtgccctc gttacaggcc gacggctgct
     2941 cgacaggcgc gagaacgcgc gcagggagct cctgagccgg gccaggcaaa acagggcgga
     3001 gctaggacgg gtggcgaggg aagaccgcac cgcggcgtcg cagatgaagc gtctggtggc
     3061 gttgcaggac ggactgctcg agagctggga cctgctgccc gcggagtgca agccgctcct
     3121 gggcgatgac atctttacca tcatgggaga gatcgaggtc gcggcgcggc tcgcgcgccg
     3181 gagggccgcg ctcaggcggc acctgcagag cgtgaaccag cgctccatcg ccaggcgcat
     3241 caaggggctc gagaaggatc tggcgaagct ggagcctgac tcgcccatgc gggcgccgtt
     3301 cgagagcgcg ctggagggac gccgcggcga gctcgccggc tacccggaag tgctcgatgg
     3361 catcagcatg atcaacgccc agctcgagag cgccgagagc cttctcggca acctgcgcat
     3421 ggagcttctg gcgctggatg acagcctgat cctgggcccc caggaccctg gccttgcacg
     3481 tctgaaggag cgcatagctt acttccgtca cggcctcgac gaggtgacgc gacgggtcga
     3541 gaccctaccc gctacggaaa ggatgtcggc aagatgaacg atgatcgaaa cacggcgcgc
     3601 cagagttttt tctccagggt gttcgccggg accaccgaag gagagggtac tgccttttac
     3661 tcacccgagg aggtcggtac gagtacaccg gggataggcg agcagggggc taccgcgcac
     3721 ggcttcaccg tggagagggc ggcggaggta atcaagaacc tcccgccgga ggtgccgagg
     3781 ctctccgccg tgcgtatcgt gcgggggacg ctcgaggcgg ccggcataga tatcgccgag
     3841 ctcgagtcct ccacgagggc tagggagtcg aggttgaact ccgagataga cctgagcgag
     3901 agccgcatcc agcagctcaa ggacgagacc gaagaggtca tcaggaacct cgaggacgag
     3961 atacgcaagg cccgcgaggc gagaaacttc ggcgtctcgg aggaggagcg caagatccag
     4021 tccgccgaga cgggcctgga taacatagac ctggtgcgtg agttcttcgg cctgccgcag
     4081 gaagaaggtg aaccgcccgt cgccgctgcc gccgtcgacg acacggcaga ccacaccgcc
     4141 gacacaggcg gcgaagagac gcagatattg aatggagtgg aggaagagga gggggacgag
     4201 acgcaggtgc tgcgccgccc gggcccgctc tctgattcgg aagactactg ggaagccgag
     4261 gacaaaaagg aaccgtgagc atcccacgcc gtctggggcg tctggcccag ggtttcgtct
     4321 cgagcctgca aacggacgac cggttccgcg agaggctggt acgcggtcgc gagcgcagcg
     4381 agaccataag ggatgctttc gaggcggcct ggaggggcgc ctccgaggag tggcgcaggg
     4441 ccgaagagcg ccgcacagcc gaagaggccg cccgccagaa cggggcgagt acgcgggaaa
     4501 ggaactcggg ttggcggacg gcgcccccgt ttatcccgct cagctacccg gcaaacgtgc
     4561 tctccgcgta caacaggctg ggtctcgccc caggg
//
LOCUS       DBNGVD010000125         6950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3670034, whole genome shotgun sequence.
ACCESSION   DBNGVD010000125 DBNGVD010000000
VERSION     DBNGVD010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3670034"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC16CM_03630"
     CDS             complement(<1..578)
                     /locus_tag="AC16CM_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR3138949.1"
                     /translation="MESRILSAIRARLRSRDLDAYVAYTPSNVFYATGFQSYFLLQWW
                     RMQGTVLAVIPADESVGVGMMVGDFEAGPARRVSGIDDVRSYRLWPEIRDAAAVRDPL
                     APGESEMGRPAQFDEDEQDEIMRGLLGDRGLLGARIGTDLRYMLFDSYRRFSSLAPGS
                     EWVDFTEDMYGLRSIKHPFEVERLKLATELSEA"
     gene            715..1515
                     /locus_tag="AC16CM_03635"
     CDS             715..1515
                     /locus_tag="AC16CM_03635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3138950.1"
                     /translation="MQDATRRVIDLEQPRTESMPIHPAHKQAGYRYLLHRHHEDEFDP
                     ERSGPRTGAAGVLICGEHTGTHIDALSHQADSLILCGGVSVNEVQSPRGFSRHGVEEI
                     PAIVAPGVLLDVAASAGVESLAPGRVVTASDLADCCEAQRVDVEAGSVALVRLGNARF
                     WSDEERYLAGPGMDASAARWMADKNVLAVGADNMAWDVPGLEDPELGCMMPGHLLLLA
                     RHGIYIIENLNLDELAASGHHIFELVCTPLKLVGATGSPVRPLAIVPG"
     gene            complement(1548..4169)
                     /locus_tag="AC16CM_03640"
     CDS             complement(1548..4169)
                     /locus_tag="AC16CM_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3138951.1"
                     /translation="MVEYLRGAGGGTAGGAKLDALIHGMSDAVYVGDERGVSACNEAA
                     LLMLGCKHISELEGGLSVLFEKLRHRSPETGEHVPRTEEPLSRALEGETVIEEIVARH
                     HQTGEDVLLRCAASPMREEGSIVGAIVVNTDLTDSRWSENLARSTLDSLSTHIAILDE
                     TGRISATNRAWHRFASSNPPITSSVGPGTNYLEVCDAATGKEAEDARAFARGIRDVIS
                     GRLEAFELEYPCHSPDKKRWFVGRVTRLPDTVRPQVVVAHEDVTERRLAEEEARLRAG
                     QQEAVAGLGRKALAEPDLQVLLDETVGLVARTLGVEYCRTLELLPSGDEMLLRAGIGW
                     EEGLVGNATEKTGTGSQAGYTLLSNGPVILEDLAHETRFDRSPLLLRHSVVSGMSAVV
                     GGERGPFGVIGAHTMKQRIFTEDDVNFLQAVANVLGEAIEWRRAEEGMREVREAERRR
                     MARDLHDGPLQDLTYALTAAQLAQSMTDDSNLGRWLERAVEAMKRTGQELRTAVYDLR
                     LEVESDRPLPEQVRSLVELNRRMSPDRHIRLSVAEDLPPHPLGRKDPELLRILQEALA
                     NTRRHSEAENVSVSLGVEEGRFWAEVEDDGRGFEAGTSSGIGLKSMHERARALGGALR
                     IESEPGRGTRVRFTVARGRGPQGRMSILLVEDHTSFRDAATAVFQLEPGFEVVGHAGS
                     LAEARQMIDNGTPVADVAIIDLGLPDGYGGDLIKELHSKNLQTQVLVVSGSLDRAEVA
                     RAVEAGAAGVLHKSVGMGEVIDAVRRIGAGEMLMPLEEVVDLLRLAGHQREEDREARR
                     AVARLTPREIEVLRVLAEGLDGKEIAERLGISAKTGRNHVASIMEKLGVHSRLQALVF
                     AIKHGVVDPPRRHSQRF"
     gene            4528..5334
                     /locus_tag="AC16CM_03645"
     CDS             4528..5334
                     /locus_tag="AC16CM_03645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3138952.1"
                     /translation="MSETAPTAPDTVVLVHGFWVTPRSWEKWVERYEDKGYRVLAPAY
                     PGFEVEVEALREDPSPIEALTIPAITDHFEAVMDELDRPPIIIGHSAGGLITQILLDH
                     GYGAAGVAIDSVPAEGIRVSPVSQIRAIFPILRNPANRHKAVGFTPEQWHYAFTNTQS
                     REESDEVYERYHIPASGRLVWAGPLANFTPGHQETYVDFRNEDRAPLLFIAGSEDNIM
                     PPAVNQSNVKHYRNTRTPTDYREFEGRSHYGVIGGEGWEEVADFALEWAV"
     gene            complement(5434..6591)
                     /locus_tag="AC16CM_03650"
     CDS             complement(5434..6591)
                     /locus_tag="AC16CM_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3138953.1"
                     /translation="MVDDSKLPLAGVRVLDLSRVLAGPYATMVLGDLGADVIKVEHPD
                     RGDDTRHWGPPFAGEGEARESAYFLAINRNKRSIGVDLKDAEGLGRIKHLAAGADVVI
                     ENWRRGALERLGLGYDALRETNPGLIYCSITGFGPGPDEERPGYDFLVQARGGVMGIT
                     GQPGGAPTKVGVAISDIVCGLFASNAILAALHRRHVTGQGARIEVPLFESTLGWLANR
                     GQEYLVSGEDSGLIGNAHPSIVPYQTFDASDRPFVVAVGNNSQFAALCEAVGRPELAE
                     DDRFATNPDRVANREALIAELQEEIGKRPADEWAEEVRAAGVPSGPVNTLADVFADDH
                     VRGSGMLQDIIHASVGALKMLASPILIDSERLPIRRPPPTLGQHTDELSDW"
BASE COUNT         1208 a         2358 c         2149 g         1235 t
ORIGIN      
        1 gcctcggaga gctcggtcgc cagcttgagc cgctcgacct cgaagggatg ctttatgctc
       61 ctcaacccgt acatgtcctc ggtgaagtcc acccattccg acccgggagc cagggaagaa
      121 aaacgccggt aagaatcgaa gagcatgtag cggaggtccg tccctatcct ggccccgagc
      181 aatcccctgt cgccgagcag gccgcgcatg atctcgtcct gctcgtcctc atcgaactga
      241 gcgggacggc ccatctcaga ttccccgggc gcgaggggat cccgtaccgc cgcggcgtcc
      301 ctgatctcgg gccacaatct gtaggacctg acgtcgtcta tgcccgagac cctccgcgcc
      361 ggcccggcct cgaaatcccc gaccatcatg cccaccccaa cgctctcgtc tgccgggatg
      421 acggcgagca cggtcccctg catgcgccac cactgcagga gaaagtagct ctggaatccc
      481 gtggcgtaga acacgttgga cggggtgtag gccacgtacg cgtcgagatc ccgggagcgc
      541 aaccgcgccc ggatggccga gaggatcctg ctctccacgc tcgggctcct cctttcgacc
      601 tggtacgacg cgcccgatta tagggaaagc cacggttcag cgcccaccgt tgacgccggt
      661 gcccggtgcg gccacacttg cgcggaggga tcatccgagt cgggaggaac gcgcgtgcag
      721 gacgcaacca ggagggtaat agacctcgag caaccgcgga cggaaagcat gccgatccat
      781 cccgcccaca aacaggccgg ctaccgctac ttgctccacc gccaccacga ggacgagttc
      841 gaccccgaaa ggtcggggcc ccgcacgggg gcggcgggcg tcctaatctg tggcgagcac
      901 acgggcacgc acatagacgc cctgagccac caggcagatt cgctcatcct ctgcggtggc
      961 gtctccgtca acgaggtaca gtccccgcgc ggcttctccc ggcacggcgt cgaggagata
     1021 ccggccatag tcgcccccgg cgtgctcctc gacgtggccg ccagtgccgg cgtggaatct
     1081 ctagctcccg gtcgcgtggt gacggcctcc gaccttgcgg attgttgcga ggcccaaagg
     1141 gtcgatgtcg aggccgggag cgtggcgctc gtgcggctcg gtaacgccag attctggagc
     1201 gacgaggaac ggtatctggc ggggccgggg atggacgcga gcgcggcccg ctggatggcc
     1261 gacaagaacg tgctggccgt gggggcagac aacatggcgt gggacgtgcc cgggctggaa
     1321 gaccccgagc tggggtgcat gatgccaggt cacctgctcc tgctggcgag gcacggtatc
     1381 tacatcatcg agaacctgaa cctcgacgag ctggcggcct ccggccacca catcttcgaa
     1441 ctcgtctgca cgcccctgaa gctagtcggc gcgaccggct cccccgtaag gcccctggcg
     1501 atcgtaccgg gatagggtaa ccgccccgag accgcattgg ccggagctca gaatctctgc
     1561 gaatgccttc taggtgggtc aaccacaccg tgcttgatag cgaacacgag cgcctgtagc
     1621 ctggagtgaa cgccgagttt ctccatgatg ctcgccacgt ggtttcgccc ggtcttggcg
     1681 cttatcccga gacgctctgc tatctcttta ccgtccagtc cctcggcaag cacccgcagt
     1741 acttcgatct ccctgggcgt gagccgcgcc accgcccggc gggcttcgcg gtcctcctcc
     1801 ctctggtgtc cggcgagcct gagtagatcc acgacctcct ccaggggcat gagcatctcg
     1861 ccagccccga tacggcgcac ggcgtcgatc acctcaccca tacccaccga tttgtgcagg
     1921 acccccgctg ctccggcctc gacggcccga gccacctccg cgcggtcgag gctgccgctc
     1981 acgacgagca cctgcgtttg caggttcttg ctgtgcagtt ctttgatcag gtctccacca
     2041 tatccgtcgg gaaggccaag gtcgatgatg gccacgtccg ccaccggcgt cccgttgtct
     2101 atcatctggc gcgcctcggc gagggacccc gcgtgcccca cgacctcgaa gcccggctcg
     2161 agctggaata ccgcggtggc ggcgtccctg aaagaggtat gatcctccac cagcaagatg
     2221 ctcatccggc cctgtggtcc tctgcccctg gcaaccgtga agcgtactct ggtgcctctt
     2281 cctggctcgc tctcgattcg cagggcgccc cctagcgcgc gggcccgctc gtgcatgctc
     2341 ttcaggccta tcccggagga agttccagcc tcgaaacccc gcccgtcgtc ttcgacctcg
     2401 gcccagaacc tgccctcctc tacccccagg ctcacagaca cgttttcggc ctccgagtgg
     2461 cgccgtgtgt tggccaacgc ctcctggagg atgcgcaaca gctcagggtc tttccggccg
     2521 aggggatggg ggggtagatc ctctgccacg ctgagtcgta tgtgtcggtc gggggacatc
     2581 cggcggttca gctccacgag ggacctgacc tgctcgggca agggcctgtc gctctctact
     2641 tccagcctga gatcgtagac ggcggtgcgt agctcctgcc ccgtgcgctt cattgcctcg
     2701 accgcccgtt ccagccagcg ccccaggttc gaatcgtccg tcatcgactg ggccagttgc
     2761 gcggcggtaa gagcgtaggt gagatcctgg agcggcccgt cgtgcaggtc cctcgccatg
     2821 cgcctgcgct ccgcctccct gacctcacgc atcccttcct cggcccttct ccactcgatg
     2881 gcctcaccga gcacgttggc gacggcttga agaaagttca cgtcgtcctc cgtgaagatc
     2941 ctctgtttca tggtgtgcgc cccgataacg ccgaagggac cccgctcccc gcctacgacc
     3001 gcgctcatgc cgctgaccac gctgtgacga agcagaagcg gtgacctgtc gaacctggtc
     3061 tcgtgtgcca ggtcctccag aattaccggt ccgttcgaga gcagcgtgta gcctgcctga
     3121 gagcctgtgc ccgtcttctc cgtcgcgttt ccgacgagcc cctcctccca tccgatgcca
     3181 gcgcgcaaca gcatctcgtc gcctgaagga agcagctcga gggttctgca gtactcgacg
     3241 cccagggtac gggccacgag cccgacggtc tcatccagca ggacctggag gtcaggctcg
     3301 gcgagcgcct tcctaccgag gccggcgacc gcctcctgct gtcccgcccg cagcctggcc
     3361 tcctcttccg ccagcctgcg ctcggtgacg tcttcgtggg ccaccaccac ttgcgggcga
     3421 acggtatcag gcaagcgcgt taccctcccc acgaaccagc gcttcttgtc tggagagtga
     3481 cagggatact ctagctcgaa ggcctctagc cggccggaga ttacatccct tatcccccgc
     3541 gcgaaggcac gggcatcctc ggcttccttg ccggtggccg cgtcacagac ctccaggtag
     3601 ttggtcccgg gaccgacact ggaggtgatc ggagggttgg atgatgcgaa cctatgccat
     3661 gcccggttgg tggcggagat ccggcccgtc tcgtcgagga tggctatgtg cgtagagagg
     3721 ctgtccaggg tggagcgagc caggttttcg ctccacctgc tgtccgtcag gtccgtgttg
     3781 accacaatcg cgcccacgat acttccttct tcgcgcatcg gcgacgccgc gcaccgcagg
     3841 agcacgtcct ccccggtctg gtggtgccgc gcgacgatct cctcgatgac ggtctcacct
     3901 tccagtgcgc gggatagtgg ttcctcggtc cttggaacgt gctcccccgt ctccggggag
     3961 cggtgtcgta gcttctcgaa gagaacagag aggccccctt ctagctcgga tatatgctta
     4021 cagccgagca tcagcagggc cgcttcgttg caggccgaga cccctcgctc gtcccccacg
     4081 tagacggcgt cggacatgcc gtggatgagg gcgtcgagct tggcacctcc ggcggtgccc
     4141 cccccggctc ccctgagata ttcgaccatg acctcctccg agaggtggtt cgttctaggg
     4201 ttgcccccgg gctttccttc gtcgaccgat gatggtcctg acatccatca ccattactac
     4261 agagtgaagc aatcgtatca tgggcgaacc cggctttgcg agggtgataa cctgaccccg
     4321 gcttttcgta gtgcaaacgt actacgaaaa gataaggcac acgcatcatg acaggactcc
     4381 ccgcacgagc gatgataggg atgcgatagg aaaaggtggg gttcgaaccg gacggcagtc
     4441 tctcggggag ccgtccggtt ctcttgctgc aaactcgcgc tttgtgtaac gtgaagaata
     4501 tctttgcggc agaaggagga aaagtctatg agcgagactg ccccgaccgc acctgatacc
     4561 gtcgtcctgg ttcacggttt ctgggtaacc ccgcgcagct gggagaagtg ggtcgagcgc
     4621 tacgaggaca agggctatcg ggtactcgcc ccagcctacc ctggcttcga ggtagaggtg
     4681 gaggctctgc gggaggatcc ctccccgata gaggcgctta ccatcccggc tatcaccgac
     4741 catttcgagg cggtcatgga tgagctcgac aggcctccta tcattatcgg ccactctgcg
     4801 ggggggctta tcacccagat cttgctcgat cacggatacg gggcagcggg tgtcgccata
     4861 gactccgtgc ccgccgaagg catcagggtc tcaccggtct cgcagattcg cgctatcttt
     4921 ccgatcctga ggaaccctgc caaccgccac aaggcggttg gcttcacacc cgagcagtgg
     4981 cactacgcct tcaccaacac ccagagccgc gaggagtcgg atgaggtcta cgagcgctac
     5041 catatcccag cctcgggcag gctggtgtgg gcaggcccgc tagccaactt cacgccgggc
     5101 caccaggaaa cgtacgtcga cttccgcaac gaggaccgtg cgccgcttct cttcatagcc
     5161 ggtagcgagg acaacatcat gccgccagcg gtcaaccagt caaacgtcaa gcactaccgc
     5221 aacaccagga ccccaaccga ctacagggag ttcgaagggc gttcgcacta cggtgtgatc
     5281 gggggcgaag gctgggagga ggtagcagac ttcgccctgg agtgggccgt ctagtacgcc
     5341 aggacccacc caacagcctg agcacatcct ggtcccaggg ggccggtctc gcagcatggt
     5401 ctcgaaaccc gaggctctgg ccctcgagcc gccctaccag tcggaaagtt cgtctgtgtg
     5461 ctgaccaagg gtgggagggg ggcggcggat gggtaggcgt tcgctgtcga tgaggatagg
     5521 ggaggcgagc attttcaggg cgccgaccga agcatgaatt atgtcctgca gcatgcccga
     5581 gcccctgaca tggtcgtcgg cgaacacgtc ggcgagggtg ttgacggggc cgctcgggac
     5641 cccagcggcc ctgacctcct cggcccactc gtcggcaggc cgcttgccga tctcctcctg
     5701 gagctccgct atcaacgctt cccggtttgc cacgcggtcg gggttggtgg cgaaacggtc
     5761 gtcctcggcc agctcgggac ggcccacggc ctcgcacagc gcggcgaact ggctgttgtt
     5821 gccgacggcc acgacgaagg gcctgtccga ggcgtcgaag gtctggtagg ggacgatgga
     5881 gggatgggcg ttgccgatga ggcccgaatc ctcgccgctt accaggtatt cctggccacg
     5941 attggccagc cagccgagcg tagattcgaa gagcggcacc tcgatgcggg cgccctgccc
     6001 ggtcacgtgg cgtcggtgga gggccgccag gatcgcgttc gaggcgaaca gcccgcacac
     6061 tatgtccgag atcgccaccc caaccttggt cggagcgccc ccgggctggc cggtgatgcc
     6121 catgaccccg cccctcgcct gtaccagaaa gtcgtaacca gggcgctcct cgtcaggccc
     6181 aggaccgaaa ccggtaatgg aacagtagat gaggcccggg ttcgtctccc gtagtgcgtc
     6241 gtagccgaga ccgagcctct cgagcgcccc gcgcctccag ttttcgatta ccacgtcagc
     6301 ccccgccgcg agatgcttga tcctccccag cccctccgca tccttgaggt cgaccccgat
     6361 agagcgcttg ttgcggttga tggccaggaa gtaggcagac tcccgggcct cgccctcgcc
     6421 agcgaaaggc ggtccccagt gacgggtgtc gtcgccgcga tcgggatgct cgacctttat
     6481 cacatcggcg ccaaggtcac ccagcaccat cgtcgcgtag ggcccggcca ggacccgcga
     6541 gaggtccaga accctgacgc ccgcaagggg aagcttcgaa tcgtctacca tgacgccagt
     6601 ctaacagcac gccgcttcgc ggctcttcag cacgctcggc ctagggcctc gcttgtcagc
     6661 acactacggc cttcggcctc catttgtcag cacgcttcgc ccggctccgc caggctcgct
     6721 ttcagccaga tccctctgcg gcctgcaccc ttccgcacga cagcaacggt gccgggaaga
     6781 ggtgggtagt cgaagccacc gctacggagc gagacaactg gccgaaagcg aggcgaagcc
     6841 ggcgtgctga agagcggagg caaagccgta gcgtgctaac atgcctgtgc gaaggtcggg
     6901 ctccgagaga ggtggaaaat gaccgttacg caggctggca agaacgccga
//
LOCUS       DBNGVD010000126         7615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_26228, whole genome shotgun sequence.
ACCESSION   DBNGVD010000126 DBNGVD010000000
VERSION     DBNGVD010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_26228"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1227)
                     /locus_tag="AC16CM_03655"
     CDS             complement(358..1227)
                     /locus_tag="AC16CM_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3138954.1"
                     /translation="METTIGTRRTERPAETPDLIPGYFARIDKGNLLTHREEIDLSKR
                     AKAGDDRARKKLVEKNLRLVVSVAKRYRGMGLPFEDLIQEGNVGLMRAVEKFDPDRGW
                     RFSTYATWWIRQAVQRAVADKGRTIRVPVHMGDKIRKMARAYNELSASLNREPTDEEV
                     ARRLEWTVDEVLDVKGAMPDATSLNQTLSSDGDGSELGELIEDERASEVAGAVIGEME
                     VGSLKEAVARLPERHKHVLIRRYGLGDRDTSTLAQLSDELGISRERVRQLQREAERML
                     GSGELGMVLRGAA"
     gene            1619..1879
                     /locus_tag="AC16CM_03660"
     CDS             1619..1879
                     /locus_tag="AC16CM_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138955.1"
                     /translation="MRSGCFGLVVSVGMLCAAAVIGALSMALILSFGVSALGQPDGPE
                     RIFKVIVAAAAGVAFFLVGRGVWRDLRRRPTKPEDRSGESGR"
     gene            complement(1954..2823)
                     /locus_tag="AC16CM_03665"
     CDS             complement(1954..2823)
                     /locus_tag="AC16CM_03665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3138956.1"
                     /translation="MAEVIQGIFPGSEGLDGALPGVSAVATLNLSPEPGDVEGNLLLA
                     EREIRAALAIEPALRYVVLPELFTCAYSALEFAHLYAEDAEWGESARFFTALAREVGI
                     YIAYGFPEGGAGSPAGIFGSANLVGPEGVVSTYRKRNLVGTTPEHFVFTAGTESRIVD
                     AGGLRVSLAICWDISFPEMVREPALGGADLILVPAAWREPWGFQYDLSCAARALDNGI
                     YVASANQSGDYAEAAFAIHGHVYGPDGTRVSSSFGSTSIGKVDLATALRWRTTYGNTL
                     VERLGEEPLEIVS"
     gene            3039..4568
                     /gene="asnB"
                     /locus_tag="AC16CM_03670"
     CDS             3039..4568
                     /gene="asnB"
                     /locus_tag="AC16CM_03670"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR3138957.1"
                     /translation="MCGIVGAYGGMDKETASRMLARVSHRGPDDEGQIEVAGNVLGHR
                     RLSIVDVEGGGQPLVTPDGNLYLVGNGEVYNHEEIRKTLRDGDLRTRSDNEVALRLVA
                     ERGPDALSELLGMFAFLIAREDGTFLAARDPVGIKPLYWARQNGHVRFASEMSAFDEE
                     WQPHVASFPPGHYWTPEGGLVEFGKPVPPREDLESFQGPSEPGAPIPEDILRQVRERL
                     VQTVNRQMMGDVPVGVFLSGGLDSSLVAAIAARWYGERGERLKTFAVGLEDSPDLLAA
                     RAVAEYLDTEHHESIYTAEDALRVLPTVVRTIESFDPSLVRSAVPNYILAEFTARHVK
                     VVLTGEGADEIFAGYEYLEEFRTEEELHTELVRTIEGLHDLNLQRDDRVTMAHGLEAR
                     VPFLELDMISLGLSLPAGWKLAGEGQEEKRLLRQAFDGWLPDDFLWRKKAQFGDGSGA
                     ASVLKERMEESVTVEEFERERDQVEPPLRTREEVAYYRIFAEDLGEVRPRRTIGRFAT
                     A"
     gene            4637..5362
                     /locus_tag="AC16CM_03675"
     CDS             4637..5362
                     /locus_tag="AC16CM_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HIR3138958.1"
                     /translation="MTDLGRILTATLAALILVIALRPWAMDFGLGLATRWDIWAFRLM
                     SFWVIWAITYLVLTWFLIFRSSAQQTRRWALGQRNPPRPHLSMLRSVVLRVLRAMFLA
                     TRTSSLFFIVFVSLVSMVLAISLGPEVRDLESVQGVSLAIMAALGVLSAWGILHSSYA
                     LHYAYMYYRSEESPGGLDFPGEKSPKQRDFAYFALTVGTSFAVSDVDVTDDAMRQAVL
                     GHQILSFFYNTLILATVVTMATG"
     gene            complement(5411..6046)
                     /locus_tag="AC16CM_03680"
     CDS             complement(5411..6046)
                     /locus_tag="AC16CM_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006166637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR3138959.1"
                     /translation="MSEESNTRELDRLIFFSDAVFAIVMTLLVLDIRVPDVATDEVPG
                     MVLELWPKIFSYLLSFLVIGLYWIGHHQTFRYVRSYDRTLLWLNLLFLLSISFIPFPT
                     ALLGEYGELRFSVIFYAVSLALARLLLALVWWYVFSGPIRTSDELDHGLAKFHLWRSL
                     AIPAIFVLSIGIAFFSTSAAIASWVLLVVADTLAWRLWRKRNGTRGGSATT"
     gene            6567..7490
                     /locus_tag="AC16CM_03685"
     CDS             6567..7490
                     /locus_tag="AC16CM_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-keto acid cleavage family enzyme"
                     /protein_id="HIR3138960.1"
                     /translation="MEKFIISAAITGGMTVPGQSEAIPVTPEEIVDSAVGAREAGATI
                     VHLHVREPETGKPSSDIGLFREVVGGIKDRCDVIVQPTTGGGKGMTLEERAAVLPELE
                     PEMATFNTGSFNFGLFPIAERGGDFEEWEVDYLEGTRDYIFRNTFSDMERVCGMFREA
                     GAKPELEAYDVGHLYNIKYLIGKGLLDTPVHIQFVLGVMGANAATIEQLMHMRRTATE
                     LFGDDYTWSAAGVGYPAEFHLAAMCLMLGGNVRVGLEDNLRVSADRRAGSNAELVEKA
                     VALGAMFDREPATPDEAREALGLKGRAAVAF"
BASE COUNT         1395 a         2402 c         2434 g         1384 t
ORIGIN      
        1 cgagaccagg ctgacgacgg agtcacacag catcatccgc gacaccgaag aagaagcggg
       61 gctctacccg agcgatcact acccgatagt tgtagatttt ggattagctg tcactttcta
      121 gctgtcagct atcagccagg aactctaggt tgatggcagt cttcgagaag acacgttcct
      181 ctgccatctg ccacagggac tcataaacgc cccccacgaa acgaagaaag ggagcccctt
      241 tttcggggct ccctttctca gggtcgaact cgaggcttag gcccttacac cgggcgcgtc
      301 ttcagcccga aagctgacag ctagtagctg acggcgcggt tcgcgaagcg agccgctcta
      361 agcagcgccg cgcaggacca taccgagctc gccgctgccg agcatacgct cggcctcgcg
      421 ctggagctgg cggacccgct cgcgggagat accaagctcg tcgctgagct gggcgagggt
      481 cgaggtatcg cggtctccca ggccgtagcg gcggatgagc acgtgcttgt gacgctcggg
      541 caggcgcgcg accgcttcct tgagcgatcc cacctccatc tcgcctatca cggcgccggc
      601 gacctcggag gcgcgctcgt cctctatgag ctcaccgagc tcggagccgt cgccgtcgga
      661 ggacagggtc tggttgaggc tggtggcgtc aggcatggca cccttgacgt cgagcacctc
      721 gtcgaccgtc cactccaggc gcctggcgac ctcttcgtcg gtaggctcac ggttgaggct
      781 cgcggagagt tcgttgtagg cacgggccat cttgcggatc ttgtcgccca tgtgtacggg
      841 caccctgatg gtcctgccct tgtcggcgac agcccgctgt acggcctgac ggatccacca
      901 cgtcgcgtag gtggagaacc tccagccgcg gtcggggtcg aacttctcga cggcccgcat
      961 gaggcccacg ttgccctcct ggatgaggtc ctcgaagggc agccccatgc cacggtaacg
     1021 cttggccacg ctgaccacta gcctgaggtt cttctcgacc agcttcttgc gggccctgtc
     1081 atctccggcc ttggcccttt tggagaggtc gatctcctcc ctgtgcgtta gaaggttccc
     1141 cttgtcgatg cgcgcaaagt acccggggat aagatccggg gtctcagcag gccgctcggt
     1201 tcgcctggtc cctatggtcg tctccatcga actcctcctt gctcctgtgt gatccgacac
     1261 cgctccagaa gcccgctccc cacccacacc ggcggacgca acggacccag aggggtaccg
     1321 ctcagacctc acccttctct actcaggatt gcgtggatgc gggaatacag gcaatttttc
     1381 ggctttagcc aaactcgtat tttacacacc ggagaccgct tgtcaagagc gaatcgccag
     1441 cgcacggcct acatcctggc ggcgatggac agattagcag agaagcgcgg ggcgtggaag
     1501 gggtagcgtg gcacgagcgt gatcgcttta acggggctgc gtaccgcctt cccgtccttt
     1561 tcgaaccttc tgagtatata gttaggcggt ggaacgaggc gtgtaagaga ggagcgggtt
     1621 gcgttctggg tgtttcggcc tggtggtgag cgtcggcatg ttgtgtgccg cggcggtgat
     1681 cggcgcgctg agcatggccc ttatcctctc gttcggtgtc tcggctctcg ggcaaccgga
     1741 cggcccggag aggatcttca aggtaatcgt cgccgcggcg gccggggtcg ccttcttcct
     1801 ggtggggcgc ggcgtatggc gggacctcag gcgacggccc acgaaaccgg aggaccggtc
     1861 cggagaaagc gggcgttagc agggcggcat gtcgttgggc ggttcgcgaa ccgtccctac
     1921 aggtttggga cctgaaacct gtagcctatc ccttcaggag acgatctcca ggggttcttc
     1981 tccgaggcgc tctacgaggg tgttgccgta ggtggttcgc cagcgcagtg cggtcgcgag
     2041 gtctaccttg cctatgctgg tgcttccgaa ggaagacgag acccgcgttc cgtcgggacc
     2101 gtagacgtgg ccgtggatgg cgaaggcggc ttccgcgtag tcgcccgact ggttcgcgct
     2161 tgccacgtag atcccgttgt ccagggcgcg ggcggcacac gagagatcgt actggaaccc
     2221 ccacggctcg cgccaggcgg cgggaaccag gatcaggtcc gcgccaccga gcgctggctc
     2281 cctgaccatc tcgggaaaac tgatgtccca gcagatggcg agcgagacgc gcaatccccc
     2341 ggcgtcgact atccgggact cggtgcctgc cgtaaagacg aagtgctccg gggtcgtgcc
     2401 caccaggttg cgcttgcggt aggtggagac cacgccctcg gggcccacga ggttcgcgct
     2461 gccgaagatg ccggcggggg atccggcgcc cccttcgggg aaaccgtagg cgatatagat
     2521 accgacctcc ctggcgaggg cggtaaagaa cctggcgctc tcgccccact cggcgtcctc
     2581 cgcgtagagg tgggcgaact ccagggcaga gtaggcgcag gtaaagagct cgggcagcac
     2641 cacgtagcgc agcgccggct cgatggcgag tgcagccctg atctctcgct ccgccagaag
     2701 gaggttgcct tccacatctc ccggctccgg gctcaggttc agcgtcgcca cggcggagac
     2761 gccaggcagg gcgccatcga gtccctcgct accggggaat ataccttgaa tgacctccgc
     2821 catagtcgcc tcctctctgc aggttcccac gtcaccgtgc gcgggacagg taggctaatt
     2881 ctagcccccc agacaccccg gcgatatcgg ccaggtggcg aataataacc agatccagaa
     2941 gagacgtact tgtccctcgg tagcatcgac aaccgcggtg aacgtgggta acatctaacc
     3001 tgatcaggcc agcggataca ggaggataac gaggcgacat gtgcggtatc gtcggcgcgt
     3061 acggaggcat ggacaaggag accgcctcgc gaatgctggc gcgcgtctcc caccggggac
     3121 ccgacgacga ggggcagata gaggtggccg gtaacgtgct cgggcacagg cgtctctcca
     3181 tcgtcgatgt ggagggcggc ggtcagccgc tcgtcacgcc agacggcaac ctctacctcg
     3241 tgggaaacgg cgaggtctac aaccatgaag agatcaggaa gacgctacgt gacggcgacc
     3301 tgcggacgcg ctcggacaac gaggtggcct tgcgcctggt cgccgaacgt ggccccgatg
     3361 ccctctccga gctactcggc atgttcgcgt tcctgatcgc cagggaggac ggcacctttc
     3421 tggcggcccg cgatccggtc ggcataaaac cactgtactg ggcgcgccag aacggccacg
     3481 tccgcttcgc ctctgagatg agcgccttcg acgaggaatg gcagccccac gttgcctcct
     3541 tcccccccgg ccattactgg accccagaag gcggcctcgt cgagttcgga aaaccggtcc
     3601 cgcccaggga ggatctggaa tccttccagg ggccgagcga gccgggcgcc cccataccgg
     3661 aagacatcct gcgccaggtc cgcgagcgac tcgtacagac ggtcaacagg cagatgatgg
     3721 gcgacgtgcc cgtcggcgtc ttcctctccg gtggcctcga ctcgagcctg gtcgccgcca
     3781 tcgccgcccg ctggtacggg gagcgcggag agcggctcaa gaccttcgcc gtcggcctcg
     3841 aggattcgcc cgaccttctc gccgcccgcg ccgtcgccga gtacctggat acagaacacc
     3901 acgagagcat atacacggcg gaagacgcac tacgggtgct gcccacggtg gtccgcacca
     3961 tcgagagctt cgacccctcg ctggtacgca gcgccgtacc gaattacata ctcgccgagt
     4021 ttacggccag gcacgtcaag gtcgtgctaa ccggcgaagg cgcagacgag atcttcgccg
     4081 gctacgagta cctggaggaa ttccggaccg aggaggagct gcacaccgag ctggtcagga
     4141 caattgaggg cctgcacgac ctgaaccttc agcgggacga ccgcgtcacg atggcgcacg
     4201 gcctcgaggc acgggtgccg ttcctggagc tcgacatgat ctccctcggc ctctccctgc
     4261 ccgccgggtg gaagctcgcc ggcgagggcc aggaagagaa acgactcctg cgccaggcct
     4321 tcgatggctg gctgcccgac gacttcctct ggcgcaagaa ggcgcagttc ggcgacggct
     4381 caggcgcggc ctccgtcctg aaagagcgaa tggaagagag cgtcacagtt gaagagttcg
     4441 agagggaacg cgaccaggtc gagccccccc tgcgcacccg cgaggaggtc gcctactatc
     4501 gcatcttcgc cgaagacctc ggcgaggtcc gccccaggcg caccatcgga cgcttcgcga
     4561 cagcgtgaaa tgagggagat gatgtaagct acacctcatg cgctaccgaa gtctgctaag
     4621 gggcgaggaa ggcttcttga cggacctcgg aagaatactg acggcgacgc tcgccgctct
     4681 tatccttgtc attgcgctgc gaccctgggc gatggatttc gggctcgggt tggcaacccg
     4741 ctgggatatc tgggccttca ggttgatgag cttctgggtc atctgggcaa ttacctacct
     4801 ggtgctcacc tggttcctga tattccgatc gtccgcccag caaactcgtc ggtgggccct
     4861 gggccaacga aacccgcccc gcccgcatct ttctatgctg cgctcagtgg tattgcgggt
     4921 attgcgggcg atgttcctgg ctacccggac gagctccctg ttttttatcg tgttcgtaag
     4981 cctggtgagc atggtgctgg cgatcagcct tggacccgaa gtgcgtgacc tggaatccgt
     5041 acagggcgta tcgctggcca tcatggccgc gctgggggtg ctctcggcct ggggaatact
     5101 ccatagctcc tacgcgctcc actacgccta catgtactat cgatccgaag aatcgcccgg
     5161 agggctcgac ttccccggcg agaagagtcc gaagcagcgc gattttgcct atttcgccct
     5221 gacggtgggc acgagcttcg cagtctccga cgtcgatgtg accgacgatg caatgcgcca
     5281 ggcagtgcta ggacatcaga tcctctcgtt cttctacaat accttgatcc tggccacggt
     5341 ggttacgatg gctaccggct aaggagcctg ccgggtgggt acgactacgg gcttcgccag
     5401 cgggcgacgc tcaggtcgtc gccgaccccc cgcgggtacc gttgcgtttt ctccacagtc
     5461 gccatgccag ggtgtccgcg acgaccaaca gcacccagga agctatagct gcgctcgtgc
     5521 tgaagaacgc gatgccgatc gagagcacaa agatcgcggg tatggccagg ctcctccaca
     5581 gatggaactt cgccaaccca tggtcgagtt cgtcgctggt ccttatcggt ccgctgaaga
     5641 cgtaccacca caccagcgcc agcagaagtc gcgccagagc cagggaaaca gcgtagaaga
     5701 taaccgagaa ccgcagctca ccatactcgc caagcagagc ggttggaaac gggatgaacg
     5761 agatgctcag caggaacaag aggttcagcc acaatagcgt cctgtcgtag ctcctgacgt
     5821 agcggaaggt ctgatggtgg cctatccagt agagccctat cacgaggaag ctgagcaagt
     5881 aactgaagat cttgggccac agctccaaga ccatacccgg tacttcgtcc gtcgccacat
     5941 cgggtacccg aatgtcgagt accagcagcg tcatgacgat agcgaaaacg gcatcggaaa
     6001 agaagatcag gcgatctagc tccctggtgt tgctctcctc tgacaccaaa ctctcaccgc
     6061 cttgccgcgc tcggatccca ccgttcctac cctacatggg tccggatcca ggcgctatgt
     6121 ggcagaaggg cgcctctgga ccgggtggtc gaagtaccgg gaaaaatcgt tgcgcccttc
     6181 tagagcaggc gcaggccagc ctggcggccg cgtcaccgtc gattgggagc gtgcgggtcg
     6241 tttatcagca gagagtaacc gccggtgatg ccggcctcca gcgtctcggg ctgcaaagtt
     6301 tccgccgctt ctggccttgc cgtgttctcc accttcgcct tctttctcgg tcggttttgc
     6361 atcctctgat acgatcatga ccagtcgggg gacgggctcg tcacgcgttt gcagtatttc
     6421 tctttcggat cggctattcc gcggacgcgg tcgtaagaag ggcactcgtc aggcacacgt
     6481 tcggcgctcg gtcagggtgg tctggtttgg gacggggcga gtgggtatct agtagggaaa
     6541 cggggactct accgaaggag tggggcgtgg agaagttcat catcagcgcg gcgatcacgg
     6601 gcggtatgac ggtgcctggg cagagcgagg ccattcccgt tacgccggag gagatcgtgg
     6661 actccgccgt cggggcgcgc gaggccgggg ctaccatagt acacctgcac gtgcgcgagc
     6721 cagagacggg aaagccgagc tcggacatcg ggctcttccg cgaggtggtc ggcgggatca
     6781 aggaccgctg cgacgtgatc gtgcagccta ccaccggggg aggcaagggc atgacgctgg
     6841 aggagcgggc cgccgtgctg cccgagctgg agccggagat ggcaacgttc aacacgggct
     6901 ccttcaactt cggcctcttc cccatcgccg agcggggcgg agacttcgag gagtgggagg
     6961 tagattacct ggaaggcacc cgcgactaca tcttccgcaa caccttctcc gacatggagc
     7021 gggtctgcgg catgttccgt gaggccggtg cgaaaccgga gctcgaggcc tacgatgtcg
     7081 gtcacctcta caacataaag tacctgatag gcaagggcct gctcgacacg ccggtacaca
     7141 tccagttcgt gctaggcgtg atgggcgcga acgccgccac catcgagcag ctcatgcaca
     7201 tgcgccgcac ggccacggag ctattcggcg atgactacac gtggtcggcc gccggcgtcg
     7261 gctacccggc cgagttccac ctggctgcca tgtgcctgat gctcggcggc aacgtgcgcg
     7321 tcggcctcga agataacctc agggtcagcg cagacaggcg cgcgggctcc aacgcagagc
     7381 tggtcgagaa ggccgtagca ctcggcgcca tgttcgaccg cgagccggcg acacctgacg
     7441 aggcgcgaga agctcttggc ctcaaaggcc gggccgcggt ggctttttga gcacgcttcg
     7501 gcctttcggc ctacgcttgt cagcacgctc gggctggtgc cctcgcttgt cagcacgtta
     7561 cggcccttcg ggcctccact ttcagcacgc tcccttcggt cgctttcagc cgatc
//
LOCUS       DBNGVD010000127         4029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3305301, whole genome shotgun sequence.
ACCESSION   DBNGVD010000127 DBNGVD010000000
VERSION     DBNGVD010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3305301"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16CM_03690"
     CDS             <1..182
                     /locus_tag="AC16CM_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3138961.1"
                     /translation="LDTVWGGTTGAYTNVVDLYVHYLRRKLDAGQEPSRIRTVHGAGY
                     TFQPRPEYPVGSTGP"
     gene            182..1432
                     /locus_tag="AC16CM_03695"
     CDS             182..1432
                     /locus_tag="AC16CM_03695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3138962.1"
                     /translation="MLEGMRWRLTLGYAGIFALILVFLAVAAVVGFSRELTDQQDILL
                     TQEAEDQSRNLLDNEHREVLAEGSAEYSWVALDPDGHVTARDPTAATLGTLGLPSKAL
                     AREALREDEGVSATIRGPQGKVRVVSLPMRETGEVIGVIQYARSLDGVRQTIDRLLLV
                     LIPLALGGLGAALLGGLYMAGRAMRPARESFERQRAFVADASHELKTPLTLIRADAEM
                     VLFRDHLNEEDRKLLDHALAETDRMGAVLSDLLLVARLDAGKVDLAEKPFDLAALLSE
                     EAERFGARAASKGVRLEVLTPEELMACGDAKRTGQALAVLFDNAVRFTPSGGSIAVRG
                     RSGDRWAEASVADTGPGISPNDLPRVFDRFYRAEAARTRSKGGGTGLGLAIARDLVRA
                     QGGDLLAERPRGGGAVFRLRLPSR"
     gene            1469..1672
                     /locus_tag="AC16CM_03700"
     CDS             1469..1672
                     /locus_tag="AC16CM_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138963.1"
                     /translation="MNEPVRIPENVLEELESVRRYTRVEILDIPTLRYVAMETHKPAL
                     VTWIDRHQGDYARGLLDGFRVES"
     gene            complement(1699..2454)
                     /locus_tag="AC16CM_03705"
     CDS             complement(1699..2454)
                     /locus_tag="AC16CM_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3138964.1"
                     /translation="MIDPGGALLLVIAGFVAGAINAAAGGGSLVTFPALIAVGYPPLI
                     ANVTNNVAVCPGYATGAWGYRQELGGQRGRIVPLVAVSAFGSLVGVALILVSSQQAFD
                     SVVPFLVLAACALLAAQPFIKSRIGARAKEGRRPGFALMVGQTLAAIYGGYFGAALGV
                     AVLALLGTFLDDDLQRLNALKALLQLVIGGTAAVGFALLTPVAWIAVAIVGPASLAGG
                     LIGANLAKRVSDRALRGGIVTYGVLAAIWLFLR"
     gene            complement(2510..3190)
                     /locus_tag="AC16CM_03710"
     CDS             complement(2510..3190)
                     /locus_tag="AC16CM_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138965.1"
                     /translation="MRLQLSPAWFWWMARGTLLLLGTVLLLVSCGDLEAVDTSPGCPP
                     GVGRGTSDSGSARGGARELLESRNFGATSQAAGDLERGIVDRRLVNALGELTEKHRIC
                     VQAFKKGHYFLPGVPDGPRIPKGYGEAGGLPNTHYFGRAADIWWVDGKPVEGNATDPG
                     VLKVGRILAGIPQAKRPDQIIGPPEWSRTLGYGREEGWVLAPDQLRLHEDHIHVGYVD
                     EPRVRNAR"
     gene            3314..>4029
                     /gene="pcaH"
                     /locus_tag="AC16CM_03715"
     CDS             3314..>4029
                     /gene="pcaH"
                     /locus_tag="AC16CM_03715"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR3138966.1"
                     /translation="MSEHDVDRIIGAESGVQPPYLYPDYVATRLRAPEKPLVILPATL
                     SDTRGPAFGHAPVGKLDNDLTRQHQGEPLGERIIVAGRVLDSDGRPVRRGLIEIWQAN
                     AAGRYTHKNDRHPAPLDPNFSGAGRCLTDEEGRYRFVTIKPGAYPWKNHDNAWRPAHI
                     HLSLFGEAFRSRLITQMYFPGDPLFSHDPLFQSVRDPGARRRLISTFDPDTTQAEWAL
                     GYRFDIVLEGGRATPLENHR"
BASE COUNT          733 a         1355 c         1310 g          631 t
ORIGIN      
        1 tgctcgatac cgtgtggggc ggcaccacgg gggcttacac caacgtggtg gacctctacg
       61 tccactacct gcgcagaaag ctcgacgcgg gacaggagcc ttcccgcatc cgcaccgtgc
      121 atggtgcagg ctacacgttc cagccgaggc ccgagtaccc ggtcgggagc acaggtccat
      181 aatgctggag gggatgcgct ggcgcctgac gttgggatac gcgggcatct tcgccctcat
      241 cctggtcttt ctggccgtgg ccgccgtggt cggcttctcg cgggagctga ccgaccagca
      301 ggacatcctg ctcacgcagg aggccgaaga ccagtccagg aacctgctcg acaacgagca
      361 cagggaggtg ctggccgagg gttcggccga gtacagttgg gtagcgctcg atccagacgg
      421 ccatgtcacc gcaagggacc ccaccgccgc gacgctcgga acgctcggcc ttccttcgaa
      481 ggctctcgcc agagaggcct tgcgggaaga tgagggggtc tccgcgacga tccgtgggcc
      541 gcagggcaag gtcagggtcg tcagcctgcc catgcgcgaa acaggtgagg taataggggt
      601 catccagtac gcacgttctc tggatggcgt gcggcagacc atagacaggc ttctcctggt
      661 tctgataccc ctggcgttgg gagggcttgg cgccgcgctc cttggcggac tgtacatggc
      721 gggccgggct atgcgtccgg cgcgcgagtc cttcgagaga cagcgcgcct tcgtcgccga
      781 tgcctcccac gagctcaaga cgccgctaac cctcatcagg gccgacgccg agatggtgct
      841 gttccgggat cacctcaacg aagaggacag gaaacttctg gaccacgcgc tcgccgagac
      901 cgacaggatg ggcgcggtcc tctcggacct cctgctggtc gcccgcctgg acgcgggcaa
      961 ggtcgacctc gccgagaaac ccttcgatct ggccgcgctc ctctcggagg aggccgaacg
     1021 cttcggggcg agggctgcct ctaaaggtgt tcgcctcgaa gtcctcaccc ccgaggagtt
     1081 gatggcctgc ggggacgcca agaggacggg ccaggccctg gctgtcctct tcgacaatgc
     1141 cgtcaggttc acaccctccg gcggcagcat cgcggtccgt ggccgatcag gtgaccgttg
     1201 ggcggaggcc agcgtggctg ataccggacc agggatctca cccaacgacc tgccccgcgt
     1261 cttcgaccgt ttctaccggg ccgaggccgc ccgcacccgc agcaagggcg gcggaacagg
     1321 cctcgggctg gctatcgccc gagacctcgt gcgggcgcag ggcggggacc ttcttgccga
     1381 acgccccagg ggtgggggag ccgtcttccg tctccgacta cccagccggt aagcccggaa
     1441 tccgcggccc cgcggtacac ttgggatcat gaacgaaccg gtccgaatac ccgagaacgt
     1501 gctcgaagaa ctggaatccg tacgtcgcta cacccgcgtg gagatcctgg acataccaac
     1561 ccttcgctac gtggccatgg agacgcacaa acccgccctg gttacctgga tagaccgcca
     1621 ccagggcgat tacgccaggg ggctgctgga cgggtttcga gttgaaagct gatagctatt
     1681 agctgacggc taaaagcttc aacgcaggaa gagccagatg gcggctagga cgccgtaggt
     1741 tacgattccg ccgcgcaggg cgcggtcgct cacgcgcttg gccaggtttg ctccgatcag
     1801 gccccccgcg agcgatgcgg gcccgacgat agcgacggcg atccaggcta ccggtgtcag
     1861 cagggcgaac ccgacggccg cggtgccccc gatcacgagc tggagcaacg ccttgagcgc
     1921 gttcagccgt tgcaggtcat catcgaggaa ggtgcctaga agggccagga cggccacgcc
     1981 cagtgctgcc ccgaagtagc cgccgtagat cgcggcgagc gtctggccga ccatcagggc
     2041 gaagccgggg cgtctaccct ccttggcacg agcgccgatc ctgctcttga taaagggctg
     2101 ggccgccaga agggcgcagg cggccaggac gaggaagggg accacgctgt cgaaggcctg
     2161 ctgcgagctc accaggatca gggcaacccc caccaggcta ccgaacgcgc tgaccgccac
     2221 gagcggcacg atgcgcccgc gctggccgcc gagctcctga cggtagcccc aggcacccgt
     2281 agcgtatccg ggacagacgg cgacgttgtt ggtcacgttg gcgattagcg gtgggtaacc
     2341 gacagcgatc agggctggaa atgtgacgag cgagccgcca ccggcggcgg cgttgatggc
     2401 ccccgctacg aaacccgcga tgacgagcag caacgccccg ccaggatcta tcacgggctg
     2461 gttctgctag tcgtggcgag ggcgaacatg acccgtatat tagcctgcat catcgtgcat
     2521 tgcggactcg tggctcgtcg acgtagccga cgtggatgtg gtcctcgtgc agccgcagct
     2581 gatccggcgc cagaacccaa ccctcctccc gcccgtaacc cagggtcctg gaccactcgg
     2641 gaggccctat tatctggtcc ggcctcttcg cttgtggaat gccggcgagg atcctgccca
     2701 ccttcaagac acccgggtcg gtggcgttgc cctctacagg cttgccgtct acccaccaga
     2761 tgtctgcggc cctgccgaag tagtgggtgt tgggcaggcc gccggcctca ccgtagccct
     2821 tggggatgcg cgggccgtcg ggtacgccag gcagaaagta atgacccttc ttgaaggcct
     2881 ggacgcagat gcgatgcttc tccgtcagct cgcccagagc gttcaccaga cgcctgtcga
     2941 cgataccccg ctcgaggtct ccggccgcct gggatgtggc cccgaagttg cggctctcca
     3001 gcaactcgcg cgccccgcct cgtgcagagc cggaatccga ggttcccctt ccaacccccg
     3061 gtggacatcc cgggctggta tctacggcct ccaggtctcc gcacgagacg agcagcaaga
     3121 ccgtgccgag gagcaatagc gtccctcggg ccatccacca gaaccacgca ggagaaagtt
     3181 gtagccgcac cctgacatgt tatgacgtcg gacgggaaac gtgcccccac ccgtcccgcg
     3241 agctcaggaa catcgtgaaa ccggggtatc gcgacggctg gcgagaaggt agcctgaggc
     3301 ggatggagcg agcatgagcg agcatgatgt ggacaggatc atcggcgccg agagcggggt
     3361 acaaccgcct tatctgtacc ctgattacgt tgcgacgcgc ctcagggcgc cggagaagcc
     3421 ccttgtcatc ctgccggcaa cgctctccga tacgagaggt cccgccttcg ggcacgcgcc
     3481 ggttgggaaa ctggacaacg acctcacccg ccagcaccag ggcgagccgc taggcgagcg
     3541 catcatcgtc gcggggcggg tgctcgacag cgacggtcgc ccggtaagga gaggcctgat
     3601 cgagatctgg caggccaacg ccgcgggccg ctacacccac aagaacgaca ggcaccccgc
     3661 gccgctcgac ccgaatttct ccggcgcggg ccgctgcctt accgacgagg agggacgcta
     3721 ccggttcgtg acgatcaagc cgggcgccta tccctggaag aaccacgaca acgcctggcg
     3781 cccggcccac atacacctct cgcttttcgg ggaggcgttc aggagccgcc tgataaccca
     3841 gatgtacttc cccggtgacc cgctcttctc gcacgacccc ctcttccagt cggtccgtga
     3901 ccccggggcc cgtcggcgcc tgatctccac cttcgacccg gataccacgc aagcggaatg
     3961 ggccctcggc taccgcttcg acatcgtgct cgaaggagga cgagccaccc ccctggagaa
     4021 ccacagatg
//
LOCUS       DBNGVD010000128         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_26429, whole genome shotgun sequence.
ACCESSION   DBNGVD010000128 DBNGVD010000000
VERSION     DBNGVD010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_26429"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16CM_03720"
     CDS             <1..471
                     /locus_tag="AC16CM_03720"
                     /inference="COORDINATES: protein motif:HMM:NF044751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3138967.1"
                     /translation="VRQGRQPPAEDADPVATALARNSRVMIDFDYEGSPLVSDHGANG
                     TTAEPHPGQRYPDWTRFGGVAHHLLVFGHVPDTAALGRLDRRWARLVEIIHDPDVDPA
                     RAGIPKGGVVLIRPDGHIGFRSPTTDPKALAALDQHLSSYLIPDPAANGGPERA"
     gene            complement(609..1580)
                     /locus_tag="AC16CM_03725"
     CDS             complement(609..1580)
                     /locus_tag="AC16CM_03725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3138968.1"
                     /translation="MTDRLENAAPPNAAVLAPESVLESVAPLPTAPGAPRVATPDEER
                     LERLTRVCVDDVITAFGLGGVGRGRAVLELLSRVPVRRLARQVMTFDGIVGESGLGAG
                     GAWALARMCREARVEGQSKVPHRGPLLLVSNHPGLADAVALFAATPREDLRVIAAERP
                     FLDALPNTSRYLLTVAEDSGGRSGLVRAAARHLRDGRAVLTFPGGTIEPDPAVLPGAV
                     ESLDRWSSSADLFARLVPDLTVVPVVVSGVISARALRIPLIRLRRRRRDREWLAATLQ
                     MLVPALRNVNIRVEFGDPVYAGDGGSIGQAVIKKARRLMEKNSTSYP"
BASE COUNT          298 a          609 c          582 g          260 t
ORIGIN      
        1 gttcggcagg gacgtcaacc acccgccgaa gacgcggatc cggtcgccac cgcgctggcg
       61 cgcaactcac gcgtcatgat cgacttcgac tatgagggaa gcccgctagt ctccgaccac
      121 ggggcaaacg ggaccacagc agagccgcac cccgggcagc gctaccccga ctggacgcgg
      181 ttcggcgggg tggcgcacca cctgctcgtc ttcggacacg ttccagacac ggcagcgctc
      241 ggacggctgg accgacgctg ggccaggctc gtagagatca ttcacgaccc cgacgtagac
      301 cccgcccgcg ccggcatccc gaagggcggt gtggtcctga tccggcccga cggccatatc
      361 ggcttccggt caccgactac cgacccgaaa gcgctcgcgg ctctcgacca gcacctctcc
      421 tcatacctga ttccagaccc ggccgcgaac ggcggtccgg agagagcatg aaccaccccg
      481 gaggagacta caggattcag caagaagtac ttatgcctgc agcctgaaac cttatctcgg
      541 gttaccctcc gaacagcgcc ctcgccggcg gcgtccctca taccacggcg gaagatcccg
      601 ttgctatctt acgggtagga ggtggagttc ttctccatca ggcgtctggc cttcttgatg
      661 actgcctggc cgatggagcc accatccccg gcgtagacgg ggtctccgaa ctccaccctg
      721 atgttcacgt tgcgcagggc cgggacgagc atttgcagcg tggcggccag ccactcgcgg
      781 tcgcgccttc ggcggcgcag gcggatcagg gggatgcgca gtgccctggc cgagatcacg
      841 ccgctgacca cgacgggtac gaccgtgagg tccggcacga gccgggcgaa gaggtcggcg
      901 ctcgacgacc agcggtcgag ggactcgacg gcgccgggca ggaccgcggg gtcgggctct
      961 atggtacccc ccgggaaggt cagcacggca cgcccatccc gcaggtgcct ggccgcggcc
     1021 ctcacgaggc cggaacgccc tccggagtcc tccgcgacgg tcaggaggta gcgcgaggtg
     1081 ttcggtaggg catcgaggaa gggcctctcg gcggcgataa cgcgcagatc ctctcggggc
     1141 gtcgcggcga agagggcgac cgcgtcggcg aggccggggt ggttggagac gagcagcaac
     1201 gggcctctgt ggggcacttt ggactggccc tcgacccttg cctcgcggca catcctcgcc
     1261 agcgcccacg cgccacccgc tcccaggcca gactccccga cgatcccatc gaaggtcatg
     1321 acctggcgcg ccagacgacg gaccggtacg cgtgagagaa gctccagcac ggcccgtccg
     1381 cggcccaccc caccaagtcc gaaggcggtg atcacatcgt ccacgcatac ccgcgtgagc
     1441 cgttccagac gctcctcatc aggggtggcc acccgcggtg ccccgggcgc ggtggggagg
     1501 ggggcgacgc tctccagaac actttccggg gcgagaactg cggcgttggg cggagcggcg
     1561 ttctcgagcc tgtccgtcac caggggtgcc gtcctttctg tctctccacc cgaggcccag
     1621 gaggcctcca cttcacatgt tagtacgcag aaccgaccga ggtggttcta agttctcggg
     1681 tggatcgcga ctcgtatctt gttcaggggc ctagacaacg tcctggcggc ggcgcaggat
     1741 ctcgacggc
//
LOCUS       DBNGVD010000129         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_391906, whole genome shotgun sequence.
ACCESSION   DBNGVD010000129 DBNGVD010000000
VERSION     DBNGVD010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_391906"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..950)
                     /locus_tag="AC16CM_03730"
     CDS             complement(135..950)
                     /locus_tag="AC16CM_03730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3138969.1"
                     /translation="MVSPPQNESLRYRRPAELCAALAGYVGILAERLLTSPPAYETVR
                     WEEALAELGARFGNVSEILNEPALLEVEEGTRRLLAEIHPEDPYLPRWAADSLMARLC
                     YLACRLLEPETVVETGVAYGVSSAYILTALRENGRGMLHSVDLPPLRRRSGRFWGIAV
                     DQDLRFRWRLHRGPSVSILPRLLREIPTVDLFVHDSLHTHRNMRREFDTIWPHLKEGA
                     LLLADDVERNHAFGELAQKAPALLRVVEDRETSPLHGRAAPVVFGIAMKAHSG"
     gene            1207..1833
                     /locus_tag="AC16CM_03735"
     CDS             1207..1833
                     /locus_tag="AC16CM_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR3138970.1"
                     /translation="MKWLKVYGLPRLPLFLARWAMKLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMSRIGEKQGSDLREQLGVKTMKDTVDVVMLANRFFDI
                     EITLTETKEGEYVINADRCPWFGGMGHDGVPGWDVKPCAAFSTYEKAMVNAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDAELGDGPPTGVSPEMVSIGKKPGKPEA"
     gene            complement(1878..>2583)
                     /locus_tag="AC16CM_03740"
     CDS             complement(1878..>2583)
                     /locus_tag="AC16CM_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HIR3138971.1"
                     /translation="AALEGVEGVFLACSNQPRQVEYENRVIDAARGAGVRRIVKLSAH
                     GAGIGSPVAFWGWHARIEDHLRASGVPSVVLRPTFSMANLFASAEAVKHTGKLFVPAG
                     DAGIAMIHPQDVAAVAAVALLEDGHEGEPYTLTGPEAITFFQVARYLREALGHEVEYL
                     NVPDETALQGMVEQDLPEFVAGQIVTVFGILRGGAQERTTGTVQTLTGRKPRGFSDFA
                     REHAALFGRGVSLISK"
BASE COUNT          462 a          793 c          896 g          432 t
ORIGIN      
        1 tgtgcggaga ggtgcaggct gcagcgggat ctggctgaca gcgagccagg cgaagccggg
       61 cgatgcgttg ctgacaagcg accaacggga gcgtgctgac aaggcgcgaa gcgccgtgct
      121 gacaagcgga ggcgttagcc ggagtgcgcc ttcattgcta ttccgaacac tacgggggcg
      181 gcccggccgt ggagtgggga tgtttctcgg tcctcgacaa cgcgcaagag tgctggggct
      241 ttctgtgcta gttcgccgaa ggcgtggttt ctttccacgt cgtcggccag gagcagggct
      301 ccctctttca ggtgcggcca tatggtatcg aactcgcggc gcatgtttct gtgggtgtgg
      361 aggctgtcgt ggacgaagag gtcgaccgtg ggtatctccc ggaggagacg gggcaggatg
      421 ctgacgctgg gtccccggtg tagtctccac cggaaccgga gatcctggtc caccgcgatg
      481 ccccagaaac gcccggatct cctgcgcagc ggcggcaggt ccacgctatg tagcatgccc
      541 cgtccgttct ccctcagggc cgtgaggatg tacgcggagc tcacgccgta ggccaccccc
      601 gtctcgacga ccgtctccgg ctcgagcagg cggcaggcga ggtaacacag gcgggccatg
      661 agcgaatccg cggcccagcg gggcaggtag gggtcctcgg gatggatctc cgcgaggaga
      721 cgtcgggtgc cctcttctac ctcaagtagc gcgggctcgt tcaggatctc tgatacgttc
      781 ccgaagcggg ctccgagctc ggcaagcgcc tcctcccagc ggacggtctc gtaggccggg
      841 ggtgaggtga ggagccgctc ggccaggatc cccacgtatc cggccagggc ggcgcagagc
      901 tccgccggcc ggcggtagcg gagggattcg ttctgggggg gagataccat gactttggtg
      961 cggataactc cttgctagag gactgacgca ggcggaagaa tgatacgtta tctagaggcc
     1021 tcataggatc ggggagactg ccaccagagg gtgtcagagg gtgccatgcg tgcgctccaa
     1081 gcttgccgct ctggcgggga ctgcaggctg ggggatcccg gcgggcttcc gtgttcggga
     1141 agcaacagtt gcaggacgag tataatgggg gttcctggat gcaagcgcta agaaggactt
     1201 ttcattatga agtggctcaa ggtttacggt ctgcccaggc ttccactgtt cctggcgcgg
     1261 tgggcgatga aactcgggct tcccggcccg acgtggtaca agtggaaggc ggcgagcgcg
     1321 gggacaggcg tcatcctgga cgagatgatc cgggccgccg atgaccttgg ctacgatggc
     1381 cagaaagtcg gcaagctcgc catgagcagg atcggcgaga agcaggggag cgacctgcga
     1441 gagcagctcg gcgtcaagac gatgaaggat accgtcgacg tggtcatgct cgccaaccgc
     1501 ttcttcgaca tcgagatcac gctgaccgag acgaaagagg gcgagtacgt cataaacgcc
     1561 gaccgctgcc cctggttcgg cgggatgggc cacgacggcg tccccggctg ggacgtgaag
     1621 ccctgcgccg ccttctcaac ctacgagaag gctatggtca acgctatcaa cccgaacgtg
     1681 aagctctcct acaccgagaa gcggaccacc ggcggccata cctgccgcgg cgtctaccag
     1741 taccgggacg cggagctggg cgacggcccg ccaacgggcg tctcccccga gatggtcagc
     1801 atcggcaaga agcccgggaa gccagaagcc tagcgtagcc tcccggcttc ccggcgggcc
     1861 aaaccgcccg aactgtctta cttcgagatc agggacaccc ctctcccgaa cagggcggcg
     1921 tgctcgcggg caaagtctga gaagccgcgt ggcttgcgac cggtcagggt ctgaaccgtt
     1981 cctgtggtcc tttcctgggc accgccgcgt aggatcccga acacggtgac gatctgaccg
     2041 gcgacgaact cgggtaggtc ctgctcgacc atgccctgaa gcgccgtctc atcgggaacg
     2101 ttgaggtact cgacctcatg ccccagggct tcccggaggt accgggccac ctgaaagaag
     2161 gtgatggcct cggggcccgt tagcgtgtac ggctctccct cgtgcccgtc ttcgagcagg
     2221 gctacggcgg ccacggccgc cacgtcttgc ggatggatca tggcgattcc cgcatccccg
     2281 gctggcacga acagcttgcc cgtgtgcttc acggcctcgg cggaggcgaa caggttggcc
     2341 atgctgaagg tgggccgcag aacgacggaa ggaacgcccg aggcccgcag atggtcctcg
     2401 atcctggcgt gccagcccca gaaagctacc ggggagccga tcccggcccc atgcgccgag
     2461 agcttgacga tccggcgcac accggcccct ctcgccgcgt cgatcacgcg gttctcgtac
     2521 tcgacctggc gtggctggtt ggagcacgcg aggaagacgc cttcgacgcc ttcgagcgcg
     2581 gcc
//
LOCUS       DBNGVD010000130         1538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_938536, whole genome shotgun sequence.
ACCESSION   DBNGVD010000130 DBNGVD010000000
VERSION     DBNGVD010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_938536"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="AC16CM_03745"
     CDS             complement(<1..1086)
                     /locus_tag="AC16CM_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3138972.1"
                     /translation="MARSGATNILSRETPRNLGGKEFQLERNTDIPERIATNVSARLV
                     ALRERLRPIDCTSYSRECGMTGSRAHEAMSIPARSRIRNTRRASIVLALVVALASAVA
                     ALLVAGASSSAQQRAPEASATLGTNGAPVPKIRWQDCPESVAPQPYQCASASVPLSYR
                     HPNGKTIDLALARLQATDQKRKIGTLFWNPGGPGASGRIPPPFSKELHERFDIIGFDP
                     RGTNASIPLKCFSSNRQARETFGRPFPAKLEQVNAFFAANKRGTQLCEENAGPVISHM
                     STANVARDMDLLRQAVGDQKMTYQGYSYGTFLGETYANMFPGKVRAMTLDAVLDPVEW
                     TTGEKPGVGLTQPYTYRLDSFDGAQRAL"
     gene            complement(1136..>1538)
                     /locus_tag="AC16CM_03750"
     CDS             complement(1136..>1538)
                     /locus_tag="AC16CM_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138973.1"
                     /translation="HRRARAEAFTLIESAAPPGGGLPPHVHRAQDEGIYVLEGEYTLV
                     FEDRRSILSPGSFASVPRGTTHGLKVAGDLPGRSLTILTPPGALEGFFEEVGVPVAGG
                     TIPAPPEDAPEIEEVVASARRHGMYLLRRPV"
BASE COUNT          232 a          490 c          529 g          287 t
ORIGIN      
        1 cagggcacgc tgcgcgccat cgaagctgtc cagacggtag gtgtacggct gagtgaggcc
       61 gacgccgggc ttctcgccgg tagtccactc cacgggatcg agcaccgcgt ccagggtcat
      121 ggccctcacc ttgccgggga acatgttggc gtaggtctcc ccgagaaagg tcccgtagga
      181 gtagccctgg taggtcatct tctggtcgcc caccgcctgg cgaaggagat ccatgtcgcg
      241 ggctacgttg gccgtggaca tgtgggagat taccggtccg gcgttttcct cgcacagttg
      301 cgtgccgcgc ttgttggccg cgaagaacgc gttcacctgc tccagcttcg ccggaaacgg
      361 ccggccgaag gtctctcggg cctggcggtt ggaggagaag cacttcaagg ggatactggc
      421 gtttgtgccc ctcgggtcga agccgatgat gtcgaagcgc tcgtgcagtt ccttcgagaa
      481 aggtggcggg atgcgcccgg aagccccggg tcccccgggg ttccagaaca gggtgccgat
      541 cttgcgcttc tgatccgtgg cctgcagcct ggccagggcg aggtctatcg tcttgccgtt
      601 cgggtggcga tatgagagcg gcacgctggc cgaggcgcac tgatatggtt gaggggcaac
      661 gctctccggg cagtcctgcc accggatctt cggtaccggg gcgccgttgg tcccgagggt
      721 ggcggacgct tccggtgccc gttgctgggc cgacgaggac gcacctgcga ccaagagcgc
      781 cgctacggcc gaggccaggg cgaccaccag agcgagtacg atgcttgccc gccgggtgtt
      841 gcggatacgt gacctcgccg gtatggacat tgcttcgtgc gctctagacc ccgtcatgcc
      901 gcactcacga gagtacgagg tgcagtcgat ggggcgcagg cgttcgcgga gagcgaccag
      961 ccgtgctgaa acgttggtag cgatccgttc ggggatgtcg gtgttgcgtt caagctgaaa
     1021 ctcctttcct cccaggtttc tcggggtctc cctggataag atgttggtgg ccccgctccg
     1081 cgccaccctc gagctggatg gtggcgagga aagtcctcgt tcctcgtccg gcggttcaaa
     1141 ccggcctcct gagcaggtac atcccgtgtc gccgcgcgct cgccactacc tcctctatct
     1201 ccggcgcatc ctcgggcggc gcggggatcg tcccgcccgc gaccgggaca ccgacctcct
     1261 cgaagaaccc ttccagcgcc cctggaggcg tgaggatcgt cagggatcgg cccggcaggt
     1321 cacccgctac cttcaagccg tgggttgtcc ccctgggaac tgacgcgaac gaccccgggg
     1381 agaggatcga cctcctgtct tcgaacacga gcgtgtactc gccttcgagg acgtagatcc
     1441 cttcgtcctg ggcgcggtgg acgtggggag gcagcccgcc tcctggaggc gccgcactct
     1501 cgatgagcgt gaaggcctcg gcgcgcgccc tgcggtgt
//
LOCUS       DBNGVD010000131         1964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3124487, whole genome shotgun sequence.
ACCESSION   DBNGVD010000131 DBNGVD010000000
VERSION     DBNGVD010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3124487"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="AC16CM_03755"
     CDS             <1..137
                     /locus_tag="AC16CM_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HIR3138974.1"
                     /translation="PVIGLDVWEHAYYLKYQNRRPEYIQAWWDVLNWDEAERRFQAAK
                     "
     gene            complement(309..830)
                     /locus_tag="AC16CM_03760"
     CDS             complement(309..830)
                     /locus_tag="AC16CM_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138975.1"
                     /translation="MSFVNAFNSGDQAALSRTFFLSEGPSPPDFSNGTYDPWSWYTVS
                     NIEPGGKIVSGFVTYDQRELLRYFARRHKKGERMRLLKVSLTQTGLLGRDDNVGFVYA
                     MNRSASDLDPSFGGPERIASGQGAINCENRRIFTWRMQMKAGDERSSHEAANWLCTNP
                     PGWRPGKSVVACA"
     gene            complement(1187..1810)
                     /locus_tag="AC16CM_03765"
     CDS             complement(1187..1810)
                     /locus_tag="AC16CM_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3138976.1"
                     /translation="MTPARVFVVAPTPTLRAGLRSMLAAVESADLVVVGEGAASGTGR
                     EVSDADVVIVADGGWLEETPLSVTEEGTQSLLLLSDDEDVTSRLRSLSVRGWGVVPPD
                     APSEELGAAVAAVAQGLTVLPKTLAERLLDEPAAPVEELSDPLTARESQVLGLLGGGL
                     SNKMIARELHISEHTVKFHISSLYSKLGVSNRAEAVSQGARHGLLSL"
BASE COUNT          369 a          630 c          613 g          352 t
ORIGIN      
        1 cgccagttat agggttggac gtgtgggagc acgcctacta cctgaagtac cagaacaggc
       61 gtcctgagta catacaggcg tggtgggatg tgctgaactg ggacgaggcc gagaggcgct
      121 tccaggcggc gaagtaggct acaggataaa ggtttcaggc aatggtctgg aggggccgcg
      181 tggtatccac gcggcccctc cgatcgagag cgaacagatg cgcggtggcg ggacgaggag
      241 tccgtttgtt cctcacagat attctggcgc aagaagctga tggctgaaag ctgatacctg
      301 acagctcctt acgcgcatgc gacgacggat ttgcctggtc tccagcctgg ggggttggta
      361 cacagccagt tggcagcctc gtggctggag cgttcatccc cggccttcat ctgcatgcgc
      421 caggtgaaga tgcggcggtt ctcgcagttt atggcgcctt ggcccgaggc tatgcgctcc
      481 ggcccgccga agcttgggtc gaggtcggat gcggatctgt tcatagcgta gacgaaaccg
      541 acgttgtcgt ccctgccgag taggcccgtc tgcgtgaggc tcaccttgag cagccgcatg
      601 cgctctccct tcttgtgcct cctggcgaag tagcggagca gctctctctg gtcgtaggta
      661 acgaagccgc tgactatctt gccgccgggc tcgatgttgc taacggtgta ccaggaccag
      721 ggatcgtagg ttccgttgga gaagtcgggg gggctcggtc cttccgagag gaagaatgtc
      781 ctggacaggg ctgcctgatc cccgctattg aacgcgttta cgaacgacat caaaagaccg
      841 gccacttgcc ccggatggca gccgtctgga agagaagcgt catcgctggt cacgatcacc
      901 cgctccggct gccggtaaga gacgatctca ggagcctttc ccccagaacg cgcctttccc
      961 ttctcttcct ccccgcaacc gcccaacagc gcgagaacaa ggacaaagga gaccgccgct
     1021 atcctggaga gccttgaaat agcccaacct tccgccaacc caagctatcc gccttaccat
     1081 tctacccccg cagcacggac cacgccccac aggaaaaacc ctgacaagca agggcgtaag
     1141 cccgagcgtg ctgacaagcg gaggtccgga gggccgtagc gtgccctcag agcgagagta
     1201 ggccgtggcg tgcgccctgg cttacggctt cggcgcggtt gcttacaccg agcttggagt
     1261 agagcgagga gatgtggaac ttgaccgtat gctcgctgat gtgtagctcg cgtgcgatca
     1321 tcttgttcga gaggccgcct ccgagcaggc cgagaacctg gctctcccgg gcggttagcg
     1381 ggtcggatag ctcttcgacg ggcgcagcgg gttcgtcgag gagccgttcg gccagcgttt
     1441 tcgggagcac ggtgagcccc tgtgctacgg cggcgacggc cgcccccagc tcctccgacg
     1501 gcgcgtccgg cggcaccacc ccccagcccc ttacggacag ggagcgcaac ctggacgtca
     1561 catcctcgtc gtcggaaagc aagagtagcg actgcgtccc ctcctccgtg acggagagcg
     1621 gggtctcctc gagccaacca ccatcggcga cgatcactac gtctgcatcg gagacctcgc
     1681 gcccggtccc cgacgcggca ccctcgccga ccactaccag gtccgcactt tctacagccg
     1741 cgagcatcga acgcagtccc gcacgcaagg ttggcgtcgg ggccacaacg aacacccgcg
     1801 ccggggtcat gcggcgcgtc ccgattccac cagggaaacg ttcatgacct cgattctacc
     1861 gccgcgtagg atcctcgatt cgacggcatc tccggccctt gccagcgcct ccagcagcgt
     1921 ctccgcccca tcgagcggtt caccctcgat ggagagaagc acgt
//
LOCUS       DBNGVD010000132         9099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3124831, whole genome shotgun sequence.
ACCESSION   DBNGVD010000132 DBNGVD010000000
VERSION     DBNGVD010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3124831"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3000
                     /locus_tag="AC16CM_03770"
     CDS             <1..3000
                     /locus_tag="AC16CM_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3138977.1"
                     /translation="LDRGWPERRAEDRNDTPDQIASILESISDGFFAVDEDWRFTYVN
                     ARAEELWGRPREEMLGKNIWKVFPQAEGSEARRQIERAMQEGATTEFETLSAVLGTYV
                     SGRAYPSEHGLSVYFQDATERRRATDRLLALAAASRTFAEATLDHETLLRNVAEHVAE
                     ATGDACTVRLLSEDGTRLRPVAAHHPDPDLKRAIWEVVRDTDDRADSGLWRPVIEEER
                     VVRVKVPSRGMPSDTSEAQAEFIRRHPISEIMGAPLVTRGRLLGGISLVRYDRSEAFD
                     EGDERFLRDLADRAALAIDNAALYQRSREEAAEREQAQERSRRLAAIVESSEDAILSK
                     TLDGTITSWNGGAERLYGYRAEEVIGESVTILVPPELPREIPEILEKIGRGQKIEHFD
                     TVRVTRDGRRFNVSLSVSPLQDEAGNIVGASAIARDLTERKRMEGDLQRTLKELADMK
                     FALDESAIVAITNQRGEITYANEKFCEVSGYTRDELIGHDHRIINSGYHPKEFVSELW
                     RTIARGRVWRGELRNRAKDGSIYWVDTTIVPFLDDRGKPYQYVAIRYETTERKRAEQE
                     LREAREAERDRISRELHDVVLQDLTYALQSVRLSKKSEVGNDESRLEEAAAAMTRAVR
                     GLRSAVYDLSLEAEEGNLILSLESLVELNRGMNPGCQYRLEVGAGFPETLPQGCSKDV
                     LRVVQEALANVRRHSDARHVTVIASTGDGSAWLEVSDDGGGFDQTEVPVGTGTRGMLQ
                     RAKALGADLRVWSQPGEGTRVRLELPRQDTSTHPDRTGGEEVRVLLVDDHASFRQGVA
                     AALEAEPGLRVAGQAGSLAEARAMPGEFDVAVVDLGLPDGFGGDLIRELRAANPHAQA
                     LVLSATGERSEIARAVESGAAGVLHKSSEMAEVADAVRRLRAGETLMPLEEIVDLLRF
                     AGARKDEERDARQALSTLTEREREVLSLLAEGLEAQEIAARLHISAKTERNHVAKILF
                     KLGVHSRLQAVLFAGRHGLVEIGARDT"
     gene            3099..4196
                     /locus_tag="AC16CM_03775"
     CDS             3099..4196
                     /locus_tag="AC16CM_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HIR3138978.1"
                     /translation="MSVRDYDVVFVGAGLSALLLLAELRPRLPGRVAVIDPVPPSERG
                     LVHWSYWSHERTPYDRFAIGTWRQAEVAGIAPQPIAPYTMRLVRSTDVLAHLGALLED
                     VPLDWLRTTARSISRRNDKLYEISTDAGAVRASWVFDSATEVSPSFPSPLRPQAILGG
                     TGLVVEADRAVFDTRTATLFDPLDERSFAYLLPLSPSAALLESASFGPVAADEDQAPL
                     LHYLGARYPEARFAVTHEEMGTIPLGFAPSPTSGPRHILIGAKRGLVKPSAGYGVVRI
                     ARESKHLARLWREDRELPASRPVSWQWRLLDTAFLRLAVRDPRRPMALLHRTMHAIPL
                     AQTLRFIDEELPPRRLASLLSSTLPVVLRRP"
     gene            complement(4316..4921)
                     /locus_tag="AC16CM_03780"
     CDS             complement(4316..4921)
                     /locus_tag="AC16CM_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HIR3138979.1"
                     /translation="MRLPNTSHTSLPWRIHEIAPDFELEDVWALPTPGGPEDFPRLVR
                     KLAACDPSRSFPSAARALWELRDRVGELLGWDTPEAGIGPGMPGLHNRLPADLRDGPA
                     GPEFDALPASPLYLTDDEFAAEGANRTVHGVMHVGWVPDGAGGYRGQVAVLVKPNGLF
                     GKAYMAAIKPFRYLIVYPALMRMIGRDWRAPDRGCTPGARI"
     gene            complement(5102..5464)
                     /locus_tag="AC16CM_03785"
     CDS             complement(5102..5464)
                     /locus_tag="AC16CM_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138980.1"
                     /translation="MVSHADLPPTLLDLADAPWVDLDGRSLAPVFSSGGWPSGWREEV
                     LIEDLGRGWNMLRTPRWAYVEWDTGDKELYDMNNDRHQLHSLHARSDKADLMVGLSAR
                     LDALKVCSGASCRSAGIG"
     gene            complement(5501..6487)
                     /locus_tag="AC16CM_03790"
     CDS             complement(5501..6487)
                     /locus_tag="AC16CM_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3138981.1"
                     /translation="MSRKEFFALGGAGAAVAGLSLTGTIGGRRGGPNLLVIMTDDQPY
                     YTVTRMKAVRELIGNRGMTFSPNAYVSTPICGPARATLLTGRYSHNTGLTRTAGAYED
                     LRTSVYESDTIATRLGRAGYRTYFGGKYTNGYDGGAVPPGWDDWFAMVESEPEKAGSF
                     LYHDGSGIRRYYRSVVNETDVLGADAERFVRDRSGSSVPWFAYVCPHAPHSPYYPAPR
                     HDDQSSATPPGGVPSSGEKDLSDKPDWVRAEAPYTGSERRRATRVYRGKLGEIQEVDD
                     LVRRLMEALDQTGQQENTFVFFVTDNGYLLGEHGLHRKNLPYEEASRTPFIV"
     gene            complement(6720..7322)
                     /locus_tag="AC16CM_03795"
     CDS             complement(6720..7322)
                     /locus_tag="AC16CM_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138982.1"
                     /translation="MRENQSVSQMAMEVLKRQAVLRAERTGEPFAVALGTILRTEAGK
                     RLEELSEGEHGGESADQWQTGLPRERAEERGREQLRKERRRQREIERESARRAAWESF
                     IREEKREVKLRKKGQLAALLRERLAGELSADLRQLASLDRRQAAEGLVALTSNGKVYY
                     KPVEELEEGDMEARLASIRLRETWLKDQQDRWLEYGGSTG"
     gene            7745..8698
                     /locus_tag="AC16CM_03800"
     CDS             7745..8698
                     /locus_tag="AC16CM_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3138983.1"
                     /translation="MRSFQTNTRERRGVRRLWLWLLPVVSVALVVGVVLAAGPLSLTT
                     LGNRTGQDASGSEDSTGSWIDRVFGGGGPLAEGPLNVLVLGVDTRPDDPAMGSRTDTI
                     MLVQVVPKTGDVKLLSVPRDLYIEVGSGQKDRINAAYNYGGIEQTVDAMEGYTGVTID
                     HYAIVDFEGFREVIDAMDGVRVDVGQDVFPEKWHMGEGVQRLGGRKALFYARYRGTAG
                     GDLDRMERQRRLVAALRSKALQWNIIEKLPATMKVMNENVQTDLDLDSSVALGQVLIR
                     RGKHAEVTSEQLQGTPRTLPDGAEVLIPDDDENKLILAEFR"
     gene            8789..8869
                     /locus_tag="AC16CM_03805"
     tRNA            8789..8869
                     /locus_tag="AC16CM_03805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8822..8824,aa:Leu,seq:tag)
     gene            8912..>9099
                     /locus_tag="AC16CM_03810"
     CDS             8912..>9099
                     /locus_tag="AC16CM_03810"
                     /inference="COORDINATES: protein motif:HMM:NF017508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HIR3138984.1"
                     /translation="MTANVTKLEENKVRLDVEVAPEKVRQGVEAKIRELGRQVRVPGF
                     RPGKAPRRVIENRLGRDY"
BASE COUNT         1628 a         2946 c         2905 g         1620 t
ORIGIN      
        1 ctggacagag gatggcccga gcgccgggct gaggatagaa acgacacccc cgaccaaata
       61 gcgtctatcc tcgagagcat ctccgatggt ttcttcgccg tcgatgagga ctggcgattt
      121 acctacgtga acgccagggc tgaggagctc tggggccgcc cgcgggaaga gatgctcggt
      181 aagaacatct ggaaagtgtt cccacaggca gaggggtcgg aggctcgccg ccagatcgaa
      241 cgggccatgc aggaaggcgc gaccaccgag ttcgagaccc tgtcggcggt tctcggcacc
      301 tacgtgtcgg gacgggcgta cccttccgaa cacgggctct cggtctactt ccaggacgcg
      361 accgaacgcc ggcgggccac ggatcgattg ctcgcgctcg ccgcggcctc ccgcacgttt
      421 gccgaggcga cgctcgatca cgagaccctg ttgcgcaacg ttgccgagca tgtcgccgag
      481 gcgaccggag atgcctgcac cgtgaggcta ctctccgaag acggcacgag gctccgtccg
      541 gtcgccgccc accacccgga tcccgacctg aagagggcca tctgggaggt cgtgcgggat
      601 accgacgatc gggccgactc gggcctctgg cgtcccgtga tcgaggagga gcgcgtggtc
      661 cgggtaaagg tgccatcccg ggggatgccg tcagatacgt ccgaggccca ggcagagttt
      721 attcggcgcc accccatctc ggaaatcatg ggggcccctc tggtcaccag gggacgcctg
      781 ctcggcggga tctccctggt gcgttacgat cgctccgagg ctttcgacga gggagacgag
      841 cgctttctcc gtgatctagc agaccgcgcg gcactggcta tagataacgc cgcgctctac
      901 cagaggtcca gggaagaggc cgccgagcgc gagcaggcgc aggagcgcag ccggaggctc
      961 gccgctatag tcgagtcttc cgaggacgcc atcctgagca agaccctgga cggtaccatc
     1021 acgagttgga acggtggcgc cgagaggctc tacggctacc gtgccgaaga ggtgatcggc
     1081 gaatccgtca ccatcctggt cccgcccgag cttccccgcg aaatacccga gatccttgaa
     1141 aagatcgggc gcgggcagaa aatcgaacac ttcgacaccg tgcgggtgac cagagacggg
     1201 cggcggttca atgtctcttt gagcgtctca cccttgcaag acgaggccgg caacatcgtg
     1261 ggcgcctcgg ccatcgccag ggacctgacc gagcgcaagc gcatggaggg ggatctgcag
     1321 aggacgctca aggagctcgc cgacatgaag ttcgccctcg acgagagcgc catcgtcgcc
     1381 ataacgaacc agcgcggtga gatcacctac gccaacgaaa agttctgcga ggtctcgggg
     1441 tacacgaggg acgagctgat aggccacgac caccgcatca tcaactccgg ctatcacccg
     1501 aaggagttcg taagtgagct gtggagaacc atcgcgcgtg gcagggtgtg gcgcggggag
     1561 ctaaggaaca gggctaagga cggctcgatc tactgggtgg acacgaccat agtcccgttt
     1621 ctcgacgata ggggcaagcc ataccagtac gtggccatcc gctacgagac cacagagcgc
     1681 aagcgggccg agcaagaatt gcgggaggcc agggaggccg agcgcgaccg tatctcgcgc
     1741 gagctgcacg acgtagttct tcaggacctg acttacgccc tgcaatcggt tcgactctcg
     1801 aagaaatcgg aagtgggaaa cgacgaatcc aggctcgagg aggcggctgc cgccatgacc
     1861 agggccgtgc gcgggttgag gagcgccgtc tacgacctga gcctcgaagc cgaggaggga
     1921 aacctgatcc tctccctgga atccctcgtg gagctaaaca ggggtatgaa cccgggctgc
     1981 cagtaccggc tcgaggtggg tgcgggattt cccgagacgc ttccgcaggg ttgctccaag
     2041 gacgtgctgc gcgtcgtgca ggaggccctc gcgaacgtca ggcgccactc ggacgcccgt
     2101 cacgtaaccg tcatcgcaag caccggggat ggctcggcct ggctggaggt gtcggacgat
     2161 ggtggaggct tcgaccagac agaggttcca gtaggtacag gcaccagggg gatgttacag
     2221 cgggcaaagg ctcttggcgc agacctgcgg gtctggagcc aaccaggcga gggaaccagg
     2281 gtgcgcctcg aactgcccag gcaagacacc agtacacacc ctgaccgaac ggggggagag
     2341 gaggtcaggg tcctgctcgt agacgaccat gcctccttcc gccagggcgt cgccgctgcg
     2401 ctggaggccg agcccggcct gagggtcgcg gggcaggcgg gatccctcgc cgaagcacgt
     2461 gccatgcccg gagagttcga cgtcgcggtg gtagatctcg ggcttcccga cggtttcggc
     2521 ggtgacctga tccgggaact tcgcgccgcc aacccgcacg cgcaggcgct cgtcctgagc
     2581 gcgaccggcg agcgttcgga gatcgcgagg gccgtagaat cgggggcggc tggcgtactg
     2641 cacaagagct ctgagatggc cgaggtggca gacgccgtca ggcggcttcg cgccggcgag
     2701 acattgatgc ccctcgagga gatagtagat ctcttgcgct tcgcgggcgc gcgaaaggac
     2761 gaggagcggg atgccaggca ggcgttgtcc acccttaccg agcgggagcg ggaggtgctc
     2821 tcgcttctgg cagaaggcct ggaggcacag gaaatcgccg cgaggctcca tatcagcgcg
     2881 aagacggaac gcaaccacgt ggcgaaaata ctcttcaagc tgggcgtgca ctcccggttg
     2941 caggcggttc tattcgcagg acgtcacggc ctggtcgaga taggggcccg agatacctga
     3001 gagccactcc cgcgcttcga gacggcctgt gcccaccggt ccccgcctcg aaggccgcaa
     3061 gggctgacgt gctgcgtggc aggtagacag tttcggggat gagcgttcgg gactacgacg
     3121 tggtcttcgt cggggctggc ctctcggccc tgttgcttct ggcggagctc cggccgcggt
     3181 tgcccgggcg agttgcggtc atcgacccgg taccgccctc ggagcgaggg ctcgttcact
     3241 ggagttactg gagccatgag cggacgccct acgaccggtt tgctatcggc acctggcggc
     3301 aagccgaagt ggcggggata gccccacaac ccatcgcccc ttacaccatg cgtctggtac
     3361 gctccaccga tgtgctcgcg cacctgggcg ccctattgga ggatgttccc ctcgactggc
     3421 ttcggaccac ggcacgttcc atctcccgcc ggaatgacaa gctctacgag atctcgaccg
     3481 acgccggtgc cgtacgcgcc tcctgggtct tcgatagcgc caccgaggtt tccccctctt
     3541 ttccgtctcc acttcgaccg caggcgatcc tgggcggtac cggcctcgtg gtagaggcag
     3601 atcgcgccgt gttcgacacg aggaccgcaa ccctcttcga cccgctcgac gaacgatctt
     3661 tcgcctacct actgccgctc agcccctcgg cggcgctgct cgagtcggcc tcgttcggtc
     3721 cggttgctgc ggatgaagac caggcgccac tccttcacta cctgggagcg cggtatccgg
     3781 aggcgcgctt cgccgtcacc cacgaagaga tggggaccat accgctcggg ttcgcgccct
     3841 cgccgacgtc cgggcccagg cacatcttga tcggggccaa gcgtggtctc gtcaagccga
     3901 gcgccggcta cggcgtggtt cgcattgcca gggagagcaa gcatctggcc cgtttgtggc
     3961 gtgaggatcg agagctgccc gcgagccggc ctgtttcctg gcagtggcgg ctcctggata
     4021 ccgcgtttct gcgcctcgcc gtgagggatc cccgccgccc catggcattg ctccatcgca
     4081 ccatgcacgc gataccgctc gcccagacgc ttcgctttat cgatgaagag cttccgccac
     4141 gacggttagc gtccctcctc tcgtcaaccc tgcccgtcgt gctgcggcga ccctagcgcc
     4201 cgtttccaga gcgatggatc atctccgacg cgtcgccagg atgtcccgaa gggggtataa
     4261 cgctgcctga acacctccct ctttttggac gaactggcat cggcaccttt ggttgctaga
     4321 tccgtgcgcc aggtgtgcac ccccggtccg gcgcccgcca gtctcgcccg atcatccgca
     4381 tgagagccgg atacacgatc aggtaccgga acggcttgat cgctgccatg taggccttgc
     4441 cgaacaggcc gttgggtttc accaggacgg cgacctgccc tctgtagccg cctgccccgt
     4501 ccgggaccca gccgacgtgc atgacgccgt gcacggtccg gttggccccc tccgcggcga
     4561 actcgtcgtc tgtaaggtac agcgggctgg cgggaagcgc gtcgaactcg ggccctgcag
     4621 gcccgtcgcg caggtcggct ggcagccggt tgtggaggcc tggcatcccg ggtccgatac
     4681 cggcttccgg tgtgtcccag ccaagcagct ccccgaccct gtccctgagc tcccagagcg
     4741 cccgggccgc gctcgggaag ctcctcgacg gatcacaggc ggctaatttc cgcaccagcc
     4801 ggggaaagtc ctccggccca ccgggcgtcg gcagcgccca gacatcctct agctcgaagt
     4861 cgggggcgat ctcgtgtatt cgccagggca gggacgtgtg cgaggtgttc gggagcctca
     4921 tggctcgacc tccatccgtt gggttgcgtt ctccgtcctc ccgggtctga cggacgaaag
     4981 cgatcagaag tgacatgggc gcccccgccc cgcaagatca catcccccac gcggaccacg
     5041 tatgcccgga ccgggcaacg agcgcgatgg gcaccctcgt actcctcggc ggggtacgtt
     5101 tctagccgat gcctgcgcta cggcaagatg cacccgaaca caccttgagt gcgtccagcc
     5161 gggcggatag tccgaccata aggtcagcct tgtctgacct ggcgtgaagg ctgtgaagct
     5221 gatggcggtc attgttcata tcgtagagtt ccttgtcgcc ggtgtcccac tccacgtaag
     5281 cccacctggg cgtcctgagc atgttccagc cgcgtccgag gtcctcgatg agtacctcct
     5341 cgcgccaccc ggatggccac ccgcctgacg aaaagaccgg ggcaaggctc ctcccgtcga
     5401 ggtcaaccca gggcgcgtca gcgaggtcga gcagcgtcgg agggaggtcc gcatgagaga
     5461 ccaccgcgtc gctcctggaa ccggcttgaa gcttcggccc tcacacgatg aaaggcgtgc
     5521 gggaggcctc ctcgtagggg aggttcttcc tgtgcagccc gtgctcgcca agcaggtagc
     5581 cgttgtcggt gacgaagaag acgaaggtgt tctcttgctg tcccgtctgg tcgagggctt
     5641 ccatgagccg cctcaccaga tcatccactt cctgtatttc ccctagcttt ccccggtaca
     5701 ccctggtagc ccttcggcgc tcggacccgg tgtaaggtgc ctccgcccgc acccaatccg
     5761 gtttgtccga tagatctttc tctccgctgc tcggtacgcc cccaggaggc gtggcgctag
     5821 actggtcgtc gtgcctgggg gctgggtagt acggactgtg gggagcgtgt gggcacacgt
     5881 aggcgaacca cggaaccgat gagcccgacc gatccctcac gaagcgctcc gcgtcggcgc
     5941 cgagcacgtc ggtctcgttg accaccgacc ggtagtacct cctgattcca ctgccgtcgt
     6001 ggtatagaaa cgaaccggct ttttctggtt cggactccac catcgcaaac cagtcgtccc
     6061 acccgggagg cacggcgccg ccgtcgtaac cgttggtgta cttccccccg aagtaggttc
     6121 tatatccagc ccgcccgaga cgggttgcga tggtgtccga ctcgtaaact gatgtccgaa
     6181 ggtcctcgta ggccccggcg gtcctggtga gccccgtgtt gtgagagtac ctgccggtca
     6241 gaagcgtggc gcgtgcggga ccgcagatgg gggtagagac gtaggcgttc gggctgaagg
     6301 tcatgccacg gtttccgatc agctctcgca ccgctttcat acgggtaacc gtgtagtagg
     6361 gttggtcgtc ggtcatgatg acgagaaggt tgggtcctcc tctcctgcca ccaatagtcc
     6421 cggtaaggga cagtccggca acggcagcgc ccgcgccccc gagggcgaag aattcctttc
     6481 tactcatgcg cggctcactc acaggctaat tgcagtgcac cagcacacat ggcgacgtga
     6541 gcgaaattac gcgacctcgt tgtccgctcg ataaagcggt ctcggtctgc cgaaacactg
     6601 acctactggg agtcgggcgt tcggcgatca cctgaagatg agaaaccgaa tgctgatcgt
     6661 cagtgggcta cccaagcaaa ccactgacgc ttgtcctcga ccccatctac agggggtttt
     6721 tatcccgttg accctccgta ctcgagccat cggtcctgct gatccttgag ccacgtttcg
     6781 cgcagcctta tggaggcgag cctcgcctcc atatcccctt cctcgagttc ctccacaggc
     6841 ttgtagtaca ccttgccgtt gctggtgagg gccaccagcc cctccgcggc ctgtctccgg
     6901 tccagggaag ccagttgccg cagatccgcg gatagctctc ccgctagtct ctccctcaac
     6961 aacgcggcaa gctgcccctt ctttcgtagc ttgacctccc gcttctcttc gcgaatgaag
     7021 gattcccagg ccgcccggcg agcgctctca cgttctattt ctcgctgccg tcgccgttct
     7081 tttcgcaact gttcccgccc gcgctcctcg gcccgctctc gcggtaggcc ggtctgccac
     7141 tggtccgcgc tctccccgcc gtgctccccc tcgctcaact cctccaggcg ctttccagcc
     7201 tcagttctga gaatggttcc gagcgccact gcgaatggct cgccagttcg ttcggcgcgg
     7261 aggaccgcct gccgcttcag gacctccata gccatctggg acacactctg attctccctc
     7321 atgggggttc actcctcata gatatactgc ggctcgagaa agccctcttc atgcctgaag
     7381 ccggcgatct ctggcccatg cgacgatcgc tatttggtgc tgccgcaccg acgcatgcgc
     7441 cgattgctgg acattccatc tcgtcgcttc ctggaccatt cgaggcgagg cgcgacagaa
     7501 ccgttattgt agcacgaatt actagatgag gtgcttgccc catgcggcga cattccgccc
     7561 gatgttccgg cctaccggca tctggagatt cgggggtgac ggtagcgagc caggacagga
     7621 agagggaatc tccggtcccg ccagtgatgc ccgcaacctg aagcccggat ccatcccgga
     7681 tccgccttcc tccaccgtag ccccatgttg ctttgtgcat gcccgcagat tagactttcc
     7741 tgggatgagg agcttccaga ccaacaccag agaacggcgc ggcgtgcgcc gcctgtggct
     7801 ctggttgctg ccagtcgtct ccgtcgccct ggtggtaggc gttgtgctcg ccgccggtcc
     7861 gctctcactg acaacgcttg gaaaccggac gggtcaggac gcctccggga gcgaggactc
     7921 tacgggcagc tggatcgaca gggtttttgg gggtggggga ccgctcgccg aaggaccact
     7981 caacgtgctc gtgctcggcg tggacacgcg ccccgacgac ccggcaatgg ggagccggac
     8041 tgataccatc atgctcgttc aggtggtgcc caagaccggt gatgtcaagc tactctccgt
     8101 gccgcgcgac ctctatatcg aggtcgggtc gggtcagaaa gacaggatca acgccgccta
     8161 caactatggt ggcatcgagc agaccgtcga cgccatggag ggctacacgg gcgtcaccat
     8221 agaccactac gctatcgttg atttcgaggg cttcagggag gtcatcgacg ccatggacgg
     8281 cgtgagggtg gacgtgggcc aggacgtctt ccccgagaag tggcacatgg gcgagggcgt
     8341 gcagcgtctc ggcggacgca aggccctctt ctacgcccgc tacaggggca ccgccggcgg
     8401 cgacctggac cgcatggagc gccagcgaag gctggtagcc gcgctcagga gcaaggccct
     8461 gcagtggaac atcatcgaga agctccccgc gaccatgaag gtcatgaacg aaaacgtcca
     8521 gaccgacctc gatctcgact cttcggtggc cctcggccag gtcctgatcc gccgcggaaa
     8581 acacgccgaa gtaacctcgg agcaactgca gggcacccca agaacgctcc ccgacggcgc
     8641 cgaggtcctg atccccgacg acgacgagaa caagctgatc ctcgcagagt tccggtagcg
     8701 cagcccacgt gcgcccatag cgtaaagcct cgctataagg ggggcgccga gcactctgct
     8761 gttataatcc gcgcaggatt tgtggcgagc gagagtggtg gaatggtaga cacgctagat
     8821 ttaggatcta gtgcctcagg gcgtgcgggt tcaaatcccg cctctcgcac atcgccgccc
     8881 gggacgggcc ggtcaaaagg aactggaggg catgacggct aacgtaacta aactcgagga
     8941 gaacaaggtc aggctcgacg ttgaggtggc tcccgagaag gtcaggcagg gcgttgaggc
     9001 caagatcagg gagctcggcc ggcaggttcg ggtgcccggt tttcgtcctg gcaaggctcc
     9061 ccgcagggtg atagagaacc gcctcgggcg cgactacat
//
LOCUS       DBNGVD010000133         5867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2031598, whole genome shotgun sequence.
ACCESSION   DBNGVD010000133 DBNGVD010000000
VERSION     DBNGVD010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2031598"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="AC16CM_03815"
     CDS             <1..221
                     /locus_tag="AC16CM_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR3138985.1"
                     /translation="ATAKASAPAVVEYEYKASAPAAVEYEYKATATATASAAPAAELP
                     ETGGSSLIALGAGVLLVAGGLLARRIVR"
     gene            449..1210
                     /locus_tag="AC16CM_03820"
     CDS             449..1210
                     /locus_tag="AC16CM_03820"
                     /inference="COORDINATES: protein motif:HMM:NF051347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3138986.1"
                     /translation="MAVLALLATSLYLSDYFTAEQQRLAAAGDEAGAMKASRMAVRLD
                     PFDTDALQAQSLLWRQRMNYERAALSLREAIERDPNNYLPYLTLANLRLTMGEPDAAM
                     KDFREVLELNPGATQARSSLARTLMRQGKLREAAAEFETLKKERSASYQDLYDLGRIQ
                     ARTGEAPEGVRNIKRARRMVESGRVSGPQSANQRQLLVSMNLATADALVVAGRFGQAR
                     SILAQSPSEQAPGLLELLNSDPEAYREQVVNSDVI"
     gene            1197..1925
                     /locus_tag="AC16CM_03825"
     CDS             1197..1925
                     /locus_tag="AC16CM_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR3138987.1"
                     /translation="MTSSSGRGWRGGAREDTSLSEILYVLVSRRLLVVGIVLILAGVA
                     LLFGLFREPVYVAEARISVEPRGKLESEEARLAFLEEVRGSVVTQKMLEQVRRKAGWE
                     STPEEFSERLDPETIAGRGSGSGLLIRFAGDEPGQASRAANAYAEAFVDGVKALGDRP
                     VADGVPVAEASVEQRALPGGYSPRPLIYAAFAAGAGLLLGVAVALMLEGRASGWRGVR
                     DAEMTLKVSVLGSIPDYSQKRVEG"
     gene            1927..2559
                     /locus_tag="AC16CM_03830"
     CDS             1927..2559
                     /locus_tag="AC16CM_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase family protein"
                     /protein_id="HIR3138988.1"
                     /translation="MSLGALGDAGVRELLDPTAPVRSHYEELARNLLGVEGTGIVVVT
                     SPDPGAGRTSVCFGLGAAVAGMGRRAAVVDCNLENARLHKMIGEPNFVGLSSALDDSK
                     TLEQHGYEVVPGLLVVPTGPVLSHPASIMEDEGIPRALRYLKESRDLVLLDAPVARGI
                     LGSPNLSRSFDGVLLVVHASRTPRDAAREAADDLLAAGVNLLGVVLNGCS"
     gene            2546..4189
                     /locus_tag="AC16CM_03835"
     CDS             2546..4189
                     /locus_tag="AC16CM_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3138989.1"
                     /translation="MDAPDARPAGEEVKAPRSPEAQERLDAAQDTDKRHRPGRMLAVF
                     KGLVMLALLAVTAYAMVKDGFYMDTFWLPAAAGTLALLFATLFARGFYADLPPVGILA
                     IAFLAALVAVKGLSMVWTISETETIKETLRSSMYLAFFVMALGALTSWRQVGPLIDFS
                     VLVVAAVAGYGLLQKINPLDYPVRSIDGVRMDSTLGYSNTTAIIISMGVVLALSRMTG
                     LRSFALRGLYAALLLSFLVALYLTVSRGGLGSLGIGVIILFALGGARLQMLANLLLAV
                     VPAAWLWWRMQGLPGLLQSGIRPQEKLADGTAFRNDLIVALVVAFALQAGYTVLLNRY
                     ELLPSARRTLGIVAVAGLVLVACAGTFVAVRHFGGPGAAYQSLVGNADESSNMARRLT
                     SLDISSRGDYWRVGWAAWKEHPFTGTGAGTFQFTWLEERPSLKNVKQVHNLYLEQGTE
                     TGLFAFLALVGFVVILIGYSGRAALRSTGERRLLLSGLVCALAVYLISSVAEWHWYIP
                     ASTMFFFILAGIAMKLASREDWGPMEDETPPANGGLPRA"
     gene            4203..5189
                     /locus_tag="AC16CM_03840"
     CDS             4203..5189
                     /locus_tag="AC16CM_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3138990.1"
                     /translation="MTPGGHVPEAGYAVGMERVVGLPFSRLLGRLHVDLGRGDHRNTV
                     FLAGSGRSGTTWLSGVINYRGRYRYVFEPFNPARVGAFAGFGSKQYLRPDDTREAFLK
                     PARLALSGGLRDPWMDRFNTRLVARRRLVKDIRANLMLGWMRANFPGMPIVLLLRHPC
                     AVVTSRLALGWRDNLDETMAQEDLVRDHLLPMESQIRAAADDFERHLFLWCIDNYVPL
                     RQFAPGEIHLAFYEDLLLNPEDGLRSLFKFLGLDLDDRAYEKLGRPSPLSRMGAPPPC
                     VDGWREHVSSRQLERARGILALFGLDRVYGVGPMPSPSGAYSLMEENRGSAR"
     gene            5186..>5867
                     /locus_tag="AC16CM_03845"
     CDS             5186..>5867
                     /locus_tag="AC16CM_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3138991.1"
                     /translation="MKPSVVVLAGIRWNFLWQRHQTLATLFARSGYPTVFVETTGLAF
                     PKPGKETLRNVFSRIPGSSRRGGKPPGQNNATVYAPLTAPPTPKPFRWANQRFLVPRV
                     LRDLRKIVGPRPLVIAYPPTRTTLDLISGLDPGLVLYDCSDDYEHFPGVPRDIARTER
                     ELLLRADLVSCTSPHLLEKARRVRPDAFLSGPAVDYERFAVLQDQPPAREIETVCFFG
                     DATRERIDF"
BASE COUNT          910 a         1952 c         1963 g         1042 t
ORIGIN      
        1 ccgccaccgc caaggccagc gcccccgctg ttgtggagta tgagtacaag gccagcgctc
       61 ccgctgccgt cgagtacgag tacaaggcga ccgctaccgc caccgccagc gccgccccgg
      121 ctgctgagct tcccgagacg ggtggttctt cgctgatcgc gctcggtgcc ggtgtccttc
      181 tggtggccgg cggccttctg gcccgcagga tcgtccgcta gtcgagaccg gatgtagcct
      241 gaaattctag gctcaaccgg agcgggtccc ttcgggggcc cgcttttttc gtgcctccct
      301 gatggcgtgt ggtgcggcag gtccgtgctg ggggcgaggc gctgtggggc gtggcagatg
      361 gcttgtattc gcggggtgta gtggatatcc tcatgtacgg tgagcttgct tgaaccttac
      421 tattttcaga ttttctgcgg gttttctggt ggcggttctg gccctgctgg ccacctctct
      481 ctacctctcg gattacttta ccgccgagca gcagcgcctt gccgccgcgg gggatgaggc
      541 cggggcgatg aaagcttcgc ggatggctgt gcgcctcgat cccttcgata ccgatgcgct
      601 ccaggcccag tcgcttctgt ggcgccagag gatgaactac gagagggcgg ccctgtcgtt
      661 gcgggaggcc atagagcgtg accccaacaa ctacctgccg tacctgacgc tcgcgaacct
      721 caggctcacg atgggtgagc ctgacgccgc gatgaaggac ttccgcgagg tactcgagct
      781 caaccccggg gcaacccagg cgcggagctc cctcgccagg acgctgatga ggcaggggaa
      841 gctgcgggag gccgcggcgg agttcgagac gctaaagaag gagaggagcg ccagctacca
      901 ggatctctac gacctcggca ggatccaggc gcgaaccggc gaggcgccgg agggcgtgag
      961 gaacataaag cgtgccaggc gcatggtgga atccggcagg gtgtctgggc cacagtcggc
     1021 aaaccagagg cagcttctgg tgtccatgaa cctcgccacc gccgacgcgc tcgtcgtcgc
     1081 tgggcgcttc gggcaggcgc gaagcatcct cgcccagagc ccctccgagc aggcgcccgg
     1141 tctcctggag ttgctcaact ccgacccgga ggcctaccgc gagcaggtgg tcaacagtga
     1201 cgtcatctag cggccgcgga tggcgcggcg gggccaggga agacacctcg ctctccgaga
     1261 tcctctacgt gttggtgagc cgccgcctgc tggtggttgg tatcgtgttg atcctcgccg
     1321 gcgtggccct gctcttcggg ctcttccgcg agccggttta cgtggccgag gcgaggataa
     1381 gcgtggaacc ccgaggcaag ctggagagcg aggaggccag gctcgccttc ctcgaagagg
     1441 tgcgtgggag cgtggtcacg cagaagatgc tcgagcaggt caggcgcaag gccggctggg
     1501 aatccacccc agaggagttc tccgagcgcc tggaccccga gacgatcgca ggcaggggca
     1561 gcggttccgg gttgctgata cggttcgccg gcgacgagcc gggtcaggcc tcgagggcgg
     1621 ccaacgccta cgcggaggcg ttcgtcgacg gggtcaaagc cctgggtgac aggcctgtgg
     1681 ccgacggcgt gcccgtcgcc gaggcaagcg tggagcagag ggctctgcct gggggctaca
     1741 gcccgcggcc cctgatctac gccgccttcg ccgcaggggc ggggttgctg ctcggggtgg
     1801 ccgtggcgct gatgcttgag ggaagggcca gtggctggcg gggcgtgcgc gacgccgaga
     1861 tgacgctcaa ggtgagcgtg ctcggctcca taccggacta ttcccagaag agagtggaag
     1921 gctgaggtga gcctcggagc tctgggcgac gctggggtaa gggagttgct cgacccgacc
     1981 gcgccggtga ggtcccacta cgaggagctt gcccgcaact tgcttggggt ggaggggaca
     2041 ggcatcgtcg tcgtgacgag ccccgacccg ggcgccggac gcacgagcgt ctgcttcggc
     2101 ctcggggcgg ccgtcgccgg catgggacga agggccgccg tcgtggactg caacctcgag
     2161 aacgcgcggt tgcacaagat gatcggcgag ccgaacttcg tcgggcttag cagcgccctc
     2221 gacgacagca agaccctgga gcagcacggg tacgaggtcg tccccgggtt gctcgtcgtc
     2281 cccactggcc cggtgctgtc ccacccggcc tccatcatgg aggacgaggg gatcccaagg
     2341 gcgcttcggt atctcaagga gagcagggat ctggtgctcc tcgacgcccc tgtggcgcgg
     2401 gggatactcg gctcccccaa cctgtcccgc agcttcgacg gggtcctgct agtggtccac
     2461 gcctccagga cgcccaggga cgccgcccgc gaggcggccg acgatttgct ggcggcgggt
     2521 gtcaaccttc tcggggtggt gctgaatgga tgctcctgat gccaggccgg cgggagaaga
     2581 ggtgaaagcc ccccgctcgc ccgaggccca ggagcgtctc gacgccgccc aggatacgga
     2641 caaacgccac cgcccggggc ggatgctcgc cgttttcaag gggctcgtca tgctggccct
     2701 cctggccgtc acagcctacg ccatggtgaa ggacggcttc tacatggaca cgttctggct
     2761 gcccgccgcc gccggtactc tggcgcttct attcgcgacg ctcttcgcgc gcgggttcta
     2821 cgcggatctg ccgcccgtgg ggatcctggc gatagcgttc ctggccgccc tcgtcgccgt
     2881 caagggcctc tccatggtgt ggaccataag cgagaccgag accatcaagg agaccctgcg
     2941 ctcctcgatg taccttgcct tctttgtgat ggcgcttggc gcgctcactt cctggcggca
     3001 ggtcgggccg ctcatagact tctcggtcct ggtcgtcgcc gcggtggccg gctacgggtt
     3061 gctgcagaag ataaatcccc tggattatcc ggttcgctcc atcgacggcg tgaggatgga
     3121 ctccactctg ggttactcga acaccaccgc cattatcatt agcatggggg tggtgctcgc
     3181 gttgtcgcgg atgacgggcc tgcgaagttt cgccctgagg ggactctacg cagccctcct
     3241 gctctccttc ctggtcgcgc tctacctcac cgtctcgcgc ggcggcctcg gctccctggg
     3301 tataggcgtc atcatcctct tcgccctcgg cggcgcgcgc ctgcagatgc tcgccaactt
     3361 gctgctggcg gtggtgcccg cggcctggct ctggtggcgg atgcagggcc ttccgggtct
     3421 actccagtcg ggcatcaggc cgcaggaaaa actggcagac ggcacggcct tcaggaacga
     3481 cctgatcgtc gctctcgtgg tggccttcgc cctgcaggcc ggctacaccg tgctgttgaa
     3541 ccgctacgag ctcctgccct ccgcccgccg cacgctcggc atagtcgccg tggcggggct
     3601 cgtgctcgtg gcctgtgccg gaacgttcgt ggccgtcagg cacttcggtg gaccgggcgc
     3661 cgcctaccaa tctctcgtcg gcaacgcgga cgagtcgagt aacatggcca ggaggctcac
     3721 ctccctggat atcagctcca ggggagacta ctggagggtc ggctgggcgg cgtggaagga
     3781 acaccctttc actggcaccg gcgctgggac gttccagttc acctggctcg aggaaaggcc
     3841 ctcgctcaag aacgtgaaac aggtccacaa cctctacctc gagcagggca ccgagaccgg
     3901 cctcttcgcc ttcctcgccc tggtcgggtt cgtggtcatc ctgatcggct actcgggacg
     3961 cgccgccctg cgctcgacag gggagcggcg gctgctcctc tctggcctag tctgtgccct
     4021 cgccgtgtac ctgatctcct ccgtcgccga gtggcactgg tacattccgg catcgaccat
     4081 gttcttcttt atcctcgccg gcatcgccat gaaacttgcc tcgagggagg actgggggcc
     4141 catggaggac gagaccccac ccgcgaacgg cgggctcccg cgggcctgaa gcgttctgcc
     4201 tgatgacccc aggaggtcac gttccagagg ccggctacgc cgtcgggatg gagcgggtgg
     4261 tcggccttcc cttctctcga ctgctcggac gtcttcacgt cgacctcggg aggggtgacc
     4321 acaggaacac cgtctttcta gccgggagcg ggaggagcgg gaccacctgg ctctcggggg
     4381 tgatcaacta ccggggacgc taccgttacg tcttcgagcc tttcaacccg gccagggtcg
     4441 gcgcgttcgc aggcttcggg agcaagcaat accttaggcc cgacgacacg cgcgaggcct
     4501 tcctgaagcc ggcgcgcctc gcgctatccg ggggcctcag ggacccctgg atggaccggt
     4561 tcaacaccag gctcgtggcg cggcggcgcc tggtcaagga cataagggcg aacctgatgc
     4621 tgggctggat gcgggccaac ttccccggta tgcccatcgt gctactgctc cggcacccct
     4681 gcgccgtcgt cacctcccgc ctggccctcg gctggaggga caacctcgac gagacgatgg
     4741 cccaggagga cctggtgaga gaccatcttc tgccgatgga gtctcagata agggccgcgg
     4801 ccgacgactt cgagcgccac ctcttcctct ggtgcataga caactacgtc cccctgcggc
     4861 agttcgcacc gggcgagata cacctcgcct tctacgagga cctgctcttg aacccggagg
     4921 acgggctacg gagcctcttc aagttcctgg gtctagacct cgacgaccgg gcctacgaaa
     4981 aactcggacg tccctcgccc ctgagcagga tgggtgctcc cccgccttgc gtagacggct
     5041 ggcgcgaaca cgtgagctcc agacagctag agagggcccg tgggatcctg gcgctcttcg
     5101 gcctggaccg cgtctacggc gtggggccta tgccttcccc ttctggtgcg tactcgctta
     5161 tggaagagaa ccgggggtcc gcccggtgaa gccgtcggtg gtcgtcctgg ccggcatcag
     5221 gtggaacttc ctctggcagc gccaccagac tctggccacc ctgttcgccc ggtccggcta
     5281 tcccaccgtc ttcgtcgaga caaccggcct cgccttcccg aaaccgggca aggagacgct
     5341 acgcaacgtg ttctcgcgga taccaggctc ctcgagaaga ggcggaaagc cgccggggca
     5401 gaataacgcc accgtgtacg cgccgctcac ggccccgccc accccgaagc ccttccggtg
     5461 ggcgaaccag aggttcctcg tcccgcgcgt gctgcgcgac ctgcggaaga tcgtgggtcc
     5521 ccgaccgctc gtgatcgcct acccgcccac ccgtacgacg ttggatctca tctccggtct
     5581 cgaccccggg ctcgttctct acgactgctc ggacgactac gagcatttcc ccggcgtccc
     5641 gcgagacata gccaggaccg agcgcgagct actcctccgc gccgacctcg tctcctgtac
     5701 ctcgccacac ctgctcgaga aggcgaggcg cgtcaggcca gacgcgtttc tcagcggccc
     5761 cgcggtggac tacgagcggt tcgccgtcct gcaagaccag cctcctgcgc gcgagatcga
     5821 gaccgtctgc ttcttcggcg acgctaccag ggagcgcata gacttcg
//
LOCUS       DBNGVD010000134         2963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_574265, whole genome shotgun sequence.
ACCESSION   DBNGVD010000134 DBNGVD010000000
VERSION     DBNGVD010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_574265"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC16CM_03850"
     CDS             complement(<1..1268)
                     /locus_tag="AC16CM_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR3138992.1"
                     /translation="MENLTAGHPPGIAAALAEKRAVAEDVFDHFGEGADLIVGLGNGE
                     PVTVIDALEEGAGRLSDVKIHQMFPLRERRYMHGEVEGLRHVSWFLSPANREAFRRGG
                     CDLVPNNFCDVPRLMKRSTRRSLGLAAASPPDRHGFFSLGTHAEYMAPMIGEVPLFLE
                     VNHKMPRTFGENQVHVSQIAGWCEADYQLVELTRREERDTDQRIMEYVAERVPDSATL
                     QIGIGALPNDVLGALGDHTDLGVHSELFAEGFVDLIEKGVVTGSRKTSHRNRIITTTA
                     IGNQRLYDLVDENPGVEFWPVDYTNSPENIAKEDRMVAINATLEVDFLGQCASESLGS
                     HYWSSSGGQPDFARGAMFAEHGQSFILLHSTTADESVSRIVAQLHPGAAVTTFKNLVD
                     LVVTEYGVAELRGSSIRERTRRLIGVSHPK"
     gene            1696..1917
                     /locus_tag="AC16CM_03855"
     CDS             1696..1917
                     /locus_tag="AC16CM_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138993.1"
                     /translation="MFTDYQYLLLHDRDARERDLTERRAERGRYARPPGEPSLRARLA
                     RRLFALAVAAEREETWRQVWERLEARGRL"
     gene            2028..2822
                     /locus_tag="AC16CM_03860"
     CDS             2028..2822
                     /locus_tag="AC16CM_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3138994.1"
                     /translation="MVQALELRNVEKSFKKRRGEVVRALDSVSLSVGKGEVVGILGPN
                     GSGKSTLVRVISTLVLPDAGQVEVFGIDATRHPKKVQRSMNRVSVEASFFKKLSAMEN
                     LLYGAKLYGVTSAEARPRIAEILDNIGFARKRATEPMEHLSRGMQQKIALARALLTSP
                     VLMLLDEPTTGLDPRSKKDVQAFIKRIRDAHDSSILLCTHDMDEAEELCDRVGIMMDG
                     KILALEESEELKRRYAGEANGRAPTMEEAFMAATGYSVEEASAEEE"
BASE COUNT          549 a          904 c         1000 g          510 t
ORIGIN      
        1 aacttcgggt gggagacgcc gatcagccgc ctggtcctct cgcggatgct ggaaccgcgc
       61 agctcggcga cgccgtactc ggtcacgacg aggtcgacca ggttcttgaa cgtggtgacc
      121 gcggcgccgg ggtgtagctg ggcgacgatg cgggagaccg actcgtcggc tgtggtcgag
      181 tggagcaaga tgaaagactg gccgtgttcg gcgaacatgg ctccccgcgc aaaatccggc
      241 tgaccgcccg aggatgacca gtaatggctg ccgagtgact ccgaggcgca ctggccgaga
      301 aagtcgacct cgagggtggc atttatggcc accatgcggt cttccttggc gatgttctcg
      361 ggtgagttgg tgtagtcgac gggccagaac tcaacgcccg ggttctcgtc caccagatcg
      421 tagaggcgct ggtttccgat ggcggtggtg gtgatgatcc tgttgcggtg cgaggtcttg
      481 cgggagcccg ttacgacccc cttttcgatt aggtcgacga aaccttcggc gaacagctcc
      541 gagtgtacgc cgaggtcggt gtggtctccc agggcgccca ggacgtcgtt cggcagtgcg
      601 cctatcccta tctgcaacgt ggcactgtcg ggtacgcgct cggcgacgta ctccatgatc
      661 ctctggtccg tatcgcgctc ctcacggcgc gtgagctcca ccagctggta gtctgcctcg
      721 caccagcccg cgatctggct cacgtgcacc tggttctcgc cgaaggtcct cggcatcttg
      781 tggttgacct ccagaaacag gggcacctcc ccgatcatgg gcgccatgta ctcggcgtgg
      841 gtgccgagcg agaagaaccc gtggcggtcc ggcggggagg ctgccgccag ccccagcgac
      901 cggcgggtag agcgcttcat gaggcgtggc acgtcgcaga agttgttcgg gacgaggtcg
      961 caacctcccc ggcggaaggc ttcgcggttg gccggagata gaaaccagga gacgtgcctt
     1021 agtccctcca cctcaccgtg catgtagcgg cgttcgcgca gggggaacat ctggtggatc
     1081 ttgacgtccg agagcctgcc cgctccctcc tccagcgcgt cgataaccgt taccggctcg
     1141 ccgtttccga gcccgacgat gaggtcagca ccttcaccga agtggtcgaa cacgtcctcg
     1201 gccacggcac gtttctcggc gagcgcggcc gctatgccag gcgggtggcc cgctgtcagg
     1261 ttctccacat cccctcccat ctcgtacgga cgacctccgg cggacatgtt accggatggg
     1321 cgcgggccgc gaagcccaag aggaggcggg ccagcgttat ccggacggcg cggcaagatc
     1381 ggtgttgaca ggcccgccgc ggtagggtag tgtgctcccg ttatgttgtt gtcgcggaaa
     1441 ggactgatcg gttgatgcgc gtcgctatca cgaacatagg agttccggtg ttcgggggag
     1501 aggtgcgtcc cgaggtccgc gcggtcatga tgagaacgtg agctagcagc tcgatcaact
     1561 ctcacacgag gcccccggac cacacggacc gggggcttat attttgcccg attggcaatg
     1621 gtctccggct ccgaacccca accgacggta tcggccctca tccagggcgc gacagagagc
     1681 aaaggagaca cagcaatgtt caccgactat cagtacttgt tgttgcacga ccgcgacgcc
     1741 cgcgagcggg acctcaccga gaggcgggcc gaacgcggca gatacgccag gccccccgga
     1801 gaaccctcgc taagagccag gctagcccgg cggctcttcg ccctcgcggt ggccgccgag
     1861 cgcgaggaga cctggcgcca ggtctgggaa agactggagg ccagggggcg tttgtagggg
     1921 cggcctccgg ccgctcctgc agcggtcagc aatcagccgt cagcggtggg ctttgttctg
     1981 gctgatcgct gactgctgaa agctgacagc tacaaagagg aggtgtgatg gtacaggcat
     2041 tggaactgag aaacgtagag aagagcttca agaagcggcg gggggaggtg gtacgcgccc
     2101 tcgactcggt ctcactctcg gttgggaagg gggaggttgt aggcatcctc ggccccaacg
     2161 gtagcggcaa gagcacgctg gtgcgtgtga tctccacgct cgtccttccc gatgccgggc
     2221 aggtcgaggt tttcggcatc gacgcgacgc gccacccgaa gaaggtgcag cgttccatga
     2281 accgggtgag cgtggaggcc agcttcttca agaagctctc ggcgatggag aacctgctct
     2341 acggggccaa gctctacggc gtgaccagcg ccgaggcccg gccgagaata gcggagatac
     2401 tggacaacat cggctttgcc cgcaagcggg ccacggagcc aatggagcac ctgagccgcg
     2461 gcatgcagca gaagatagcg ctcgccaggg ccctgctcac gagccctgtc cttatgctcc
     2521 tggacgagcc gacgacgggg ctggacccca gaagcaagaa ggacgtgcag gccttcatca
     2581 agaggatcag ggacgcccac gactcctcga ttctgctctg cacccacgac atggacgagg
     2641 ctgaggagct ctgcgaccgc gtcggcatca tgatggacgg caagatcctg gccctcgaag
     2701 agtccgagga gctcaagcgc cgctacgccg gcgaggccaa cgggcgggcg cccaccatgg
     2761 aagaggcgtt catggccgcc accggctact cggtcgagga agccagcgcg gaggaggaat
     2821 agagatgaga ggcccgctaa agcccctctg gctgaccgag atggtggccg tctggggcta
     2881 cgtacaacgg aactacttcc tgaccaaacg gtacttcatg tgggagatcg tctggctcac
     2941 gttcaccacg gtcaacgcca tgt
//
LOCUS       DBNGVD010000135         2599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3124859, whole genome shotgun sequence.
ACCESSION   DBNGVD010000135 DBNGVD010000000
VERSION     DBNGVD010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3124859"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1128
                     /gene="murI"
                     /locus_tag="AC16CM_03865"
     CDS             316..1128
                     /gene="murI"
                     /locus_tag="AC16CM_03865"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR3138995.1"
                     /translation="MSDPRPIGVFDSGVGGLTVLAEIRDRMPYEHTVYFGDTARVPYG
                     VRDLAEVRWFTFEIINYLIGMDVKLVVIACNTATAAALRATQRSFEVPIIGVIEPGAR
                     AAVEETRNRRVGVLATPLTASSGAYRRAVHSLDAGIEVHEQACPEFVPMIERGEVDGP
                     ELEGVARGYLEPLMDRRVGAVVLGCTHYPLISAPINRILGPEVRLISSAGQTALEVGR
                     VLSRRDILRRPRHIEDEGKATFVCSADPEEFAALGGRFLDEEIKAVAPATVI"
     gene            1197..1270
                     /locus_tag="AC16CM_03870"
     tRNA            1197..1270
                     /locus_tag="AC16CM_03870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Pro,seq:tgg)
     gene            1297..1367
                     /locus_tag="AC16CM_03875"
     tRNA            1297..1367
                     /locus_tag="AC16CM_03875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1329..1331,aa:Gly,seq:tcc)
     gene            complement(1398..>2599)
                     /locus_tag="AC16CM_03880"
     CDS             complement(1398..>2599)
                     /locus_tag="AC16CM_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3138996.1"
                     /translation="RDENWAREYVLLTLRIGKAVEARTGDGWMLDYFGPPEWKALVDA
                     EDPEPGGVLLEATHAAEETLPHQGFGAQRTRYLAKHLRALGMAARRLSGERFSLGEQA
                     AGYFDLNVDWVAEERFGEAADLMDRVLPGQGEIRERLAAWKQRHELRPSRTHLLPALF
                     ERAILEARRRTDGLVGLPEGEEVVFGNLGDEPFLALAEYQGGLRSRVLVNTHRPFNVA
                     DLLYVACHESYPGHLAEIVMKEKHLVARKGYVEELVSFLPTPRFVVSEGLALWAREIS
                     FPGNGQLEWLEENAFPEAGIEPAGDPGEIQTARDLLWGAQCNAALMLDEGRPEEEAIR
                     YLTRWALMEEAEARRSLQSLRRPFAEAYVFCYHHGRELLEPGMRGPGRDAFVRRLLTE
                     QVCPSDL"
BASE COUNT          430 a          851 c          849 g          469 t
ORIGIN      
        1 gccgcctcca ctcgagcggg ccggcaaagc cggcccgcgc acgctccggc taacgcctac
       61 gcttgtcagc acgccgcttc gcgccttttc agcacgctcc cttcggtcgc tttcagccaa
      121 taacggctgc ggcctcgccc ttccgcacag agtaccgtcg cggagggaag ccccggttac
      181 cgagcccacc gatgcgaatc gagacgactg gccgaaagcg aggcgaagcc gaagcgttgc
      241 cgacaagcga gggcgctagc ccgagcgtgc tgacaagccg cgaagcggcg tgcggaacgg
      301 atataatccg ttccagtgag tgatccacgt ccaattgggg ttttcgattc cggtgtgggc
      361 gggctgacgg tgctggccga gatccgggac cggatgccct acgagcacac cgtttatttc
      421 ggggatacgg cgagggtacc ctacggggtc agggacctgg ccgaggttcg gtggttcacg
      481 ttcgagatca tcaattatct gatcgggatg gacgtgaagc tggtcgtgat agcctgcaat
      541 acggcgacgg cggcggcgct gagggcgacg cagcggtcct tcgaggtccc tatcatcggg
      601 gtcatcgagc ccggggcacg ggccgccgtg gaagagacgc ggaacaggcg cgtgggcgtg
      661 ctggcgacgc cgcttacggc gtcgagtggg gcttacaggc gggcggttca ttcattggat
      721 gcgggtatcg aggttcacga gcaggcctgt cccgagttcg tccccatgat cgagcgcggc
      781 gaggtggacg ggcctgagct tgaaggtgtc gcgcgtggct acctggagcc gctgatggat
      841 cgcagggtcg gcgcggtcgt gctcgggtgt acccattatc ccctgatctc ggcgcccatc
      901 aaccgcatcc tcggccctga ggtgcgcctg atctcttccg ccggccagac ggctctggag
      961 gtgggccgcg tgctctcccg cagggacata ctcaggcgtc cgcgtcacat cgaggatgag
     1021 ggcaaggcga cgttcgtttg cagcgccgac ccggaagagt tcgcggctct tggaggacgc
     1081 ttcctcgacg aggagataaa agccgtcgcc ccggccaccg taatctagca gggacaccca
     1141 ttacgaccgg cgctcccggg cgtccgcgtt tgttatactc actggcggcc ttctcgcggg
     1201 gcgtggcgca gcctggtagc gcacctgctt tgggagcagg gggtcggagg ttcaaatcct
     1261 ctcgccccga cagcggagcc agcgcacagc cgatacgcgg gtgtagctca acggtagagc
     1321 cccagccttc caagctggtt atgggggttc gattcccctc acccgctctc cacggcataa
     1381 cccccgaata actctctcta caagtcagat gggcaaacct gctccgtgag caggcgcctg
     1441 acgaaggcgt cccggcctgg gccgcgcatc ccgggttcca gtagctccct gccgtggtgg
     1501 tagcagaaga cgtaggcctc cgcgaagggc cgtcgcagag attgcagcga ccgccgcgcc
     1561 tcagcctctt ccatgagcgc ccacctcgtg aggtaccgga tcgcctcttc ctcgggcctg
     1621 ccttcgtcga gcatgagtgc agcattgcac tgtgcgcccc acagcaggtc cctggcagtc
     1681 tggatctcgc cgggatcgcc ggcgggctct atgccagcct cggggaaagc gttctcctcg
     1741 agccactcta gttgcccgtt ccctgggaac gagatctctc gggcccatag cgctaggccc
     1801 tcgctcacga cgaaccgggg agtcggcagg aaggacacaa gctcttcgac ataaccttta
     1861 cgggcgacga ggtgtttctc cttcatgacg atctcggcca gatggccggg gtagctttcg
     1921 tggcaggcca cgtagaggag gtcggccacg ttgaagggcc tgtgggtgtt gacgagcacc
     1981 cgcgagcgga gcccgccctg gtattcggcc agcgccagaa acggctcgtc gcccaggttc
     2041 ccgaaaacta cttcctcacc ttcgggcagt ccgacaagac cgtcggtgcg ccgcctcgcc
     2101 tccaggatcg ccctctcgaa gagtgctggc aggagatggg tcctggacgg tcgcagctcg
     2161 tgccgctgct tccatgccgc gaggcgctcc cggatctcgc cctgtcccgg caacacgcgg
     2221 tccatgaggt ccgcggcctc cccgaatcgt tcttcggcga cccagtcaac gttcaggtcg
     2281 aaatagccgg cggcctgctc gcccaaagag aaacgttcac cggagaggcg tcgggctgcc
     2341 atgccaaggg cgcgcagatg cttggccaga taccgcgttc gttgggcccc gaaaccctgg
     2401 tgtgggagcg tctcctccgc cgcgtgggtc gcctcgagca agaccccgcc aggctcggga
     2461 tcctccgcat cgaccagagc tttccactcc ggcggtccga agtagtcgag catccagccg
     2521 tcgccggttc gcgcttctac ggctttgccg atcctgagcg tcagcaggac gtattcgcgc
     2581 gcccagtttt cgtccctgt
//
LOCUS       DBNGVD010000136         3339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2760094, whole genome shotgun sequence.
ACCESSION   DBNGVD010000136 DBNGVD010000000
VERSION     DBNGVD010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2760094"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="AC16CM_03885"
     CDS             complement(<1..854)
                     /locus_tag="AC16CM_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3138997.1"
                     /translation="MPDTRDETFGTRLRNHREAAGLSQEELAQRAGLSTNAVGQLERG
                     ERKHPYPHTVSSLADVLGLSGQERSALLALVPRRGSPTPTPNITAMPLPLTPLVGRER
                     DLLAVRTLLEGGDARLVTLTGPGGVGKTRLALGVASEPGARYPGGVVFVTLAPVSDPD
                     LLLSTVAQALGLREAGGRSVREQVHDYLRNSRSLLVLDNFEHLLGAAPEVSGLLASCP
                     SLRVLATSRAPLRLRGEQEYPVQPLPVPRLARIPVVEEVERVASVRLLVDRAGESSPG
                     FRLTQQNAA"
     gene            993..1649
                     /locus_tag="AC16CM_03890"
     CDS             993..1649
                     /locus_tag="AC16CM_03890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3138998.1"
                     /translation="MIKDRAISLEALEISSVGAAEMEEAVGVMVRGMGDNPNVVAALG
                     QERERRHGKLLHLFGAMAAAEVPGRDRDVLAARGPDGSILGVCGMMPPGRCQPGPGRQ
                     LRLLPALLTLGPRSARHTLSWLGAWSKHDPDDRHWHLGPVAVDVHLQGLGIGSMLMRA
                     FCERMDEAGEDAYLETDKEINVRFYEKFGFGVTGEEEVLGVRNWYMYRRARKRDQGSR
                     "
     gene            1674..2273
                     /locus_tag="AC16CM_03895"
     CDS             1674..2273
                     /locus_tag="AC16CM_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3138999.1"
                     /translation="MSRAGDVIENPVTGERVVVRVGTEDSGGDLLVIDGYVKPGGAVT
                     GEHVHPSIDEDFTVLRGRVGFRIDGLESVAEPGTCLHVPAGTAHDWWNAGDEEAHVRV
                     EIRPGARFEEMAINLFGLAQDGKTNSRGMPNLLQAAILAREFSDVLYFTRPPLWVQRL
                     LFGSLAWLARLLGYRGSYPGYLERGPKERVEVELWTSQV"
     gene            2354..2755
                     /locus_tag="AC16CM_03900"
     CDS             2354..2755
                     /locus_tag="AC16CM_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139000.1"
                     /translation="MVETVEKRGTRSPRHLHLREDETLYVVEGFLSLWVAGEWVEAPA
                     STAHFLPRGVEHALLAETTTARVLSFFAPAGFEGFYGEMAAAGPYDIERLVTTAARYG
                     CEIAGPTPERPRLDPAASRPRRLPRRGPSRS"
     gene            complement(2718..>3339)
                     /locus_tag="AC16CM_03905"
     CDS             complement(2718..>3339)
                     /locus_tag="AC16CM_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR3139001.1"
                     /translation="PDNGIWESRGEKQHFVYSKVMCWVAFDSLVKGVERFGLEGPVNR
                     WRAVRDEIHADVMDRGFSEEKGSFTQAYDSDVLDACALLIPRVGFLPYHDERVVSTVE
                     TIRDELTEDGFVLRYRTDESDDGLLGREGSFLICSFWLVDALRGIGRKAEAEELFERL
                     LSLRNDVGLLSEEYDTRANRFVGNFPQAFTHLALVNCAMALSEGDA"
BASE COUNT          551 a         1048 c         1203 g          537 t
ORIGIN      
        1 gcagcgttct gctgtgtgag cctgaagcct ggggatgact ccccggcccg atccaccagg
       61 agcctcacgg aggctaccct ttccacctcc tcgacgaccg gaatgcgcgc caggcgtggt
      121 acgggaaggg gttgcacggg gtactcctgc tcgcctctca gacgcagggg tgcccggctc
      181 gtcgccagca ccctcaacga agggcacgaa gcgagcagac ccgagacttc cggcgcggcc
      241 ccgagcaggt gctcgaagtt gtccaggacc aggagcgatc gactgttccg taggtagtcg
      301 tgcacttgct cccgcacgga gcggccaccg gcttcccgta gcccgagggc ctgggccacg
      361 gtggagagga ggaggtcggg atccgagaca ggcgcaagcg tcacgaacac gacgccaccg
      421 gggtatcggg cccctggctc gctggccacg ccaagggcga ggcgtgtctt tcccactccc
      481 ccaggcccgg tgagggtgac gagcctcgcg tcacccccct cgagcaaggt gcgcaccgcg
      541 agcaggtcac gttcacgtcc gacgagcggg gtgaggggga gcggcatggc cgtgatattc
      601 ggggttgggg tcggcgaccc gcgtcgtggc accagggcga ggagcgccga gcgttcctgc
      661 ccggacagcc ccagaacgtc ggcgagggag ctcacggtgt gcgggtaggg gtgttttctc
      721 tcgccccgct ccagctggcc cacggcgttg gtgctgagcc cggccctctg cgccagctcc
      781 tcctggctga ggccggcggc ctcccgatgg ttcctgaggc gcgtgccaaa cgtttcgtct
      841 ctggtatccg gcatcccgac tcctcctgat ccgcatggta gcacggccac tcatccgcac
      901 tgagtaggcc ctgagtgggg cgtgtgggtg gtctccgctc tccccggggc gcagagtgat
      961 accagcaagc gagccaggca aaggagagaa ggatgatcaa ggacagagcc attagcctcg
     1021 aggcgctgga gatcagtagt gtaggcgccg cggagatgga agaggcggtc ggggttatgg
     1081 tacgcggtat gggcgacaac cccaacgtcg tcgcggcttt ggggcaggag cgggagcggc
     1141 gccacgggaa gctgctgcac ctcttcggtg cgatggccgc ggcggaggtg cccggccgtg
     1201 accgggacgt gctagccgcg cgcgggcccg acggttcgat cctgggggtg tgcggcatga
     1261 tgcctcccgg gcggtgccag ccgggaccgg gacggcaact gcgcctcctc cccgccctgc
     1321 tcacgctcgg tccgcggagc gccaggcaca cgctgagctg gctcggggcc tggtcgaagc
     1381 acgaccccga tgatcgtcac tggcacctcg ggcccgtggc ggtagacgtc cacctgcaag
     1441 gtctgggtat cggttcgatg cttatgcggg ctttctgcga gaggatggat gaggcggggg
     1501 aggatgccta tctggagacc gacaaggaga tcaacgtccg cttctacgag aagttcggtt
     1561 tcggggtgac gggcgaggag gaagtgctgg gcgtccgcaa ctggtacatg taccggcggg
     1621 cgcggaagag ggatcagggc tcccggtagc acggggtgcg gaaggaggac aagatgagca
     1681 gagcgggaga cgtgatcgag aaccccgtta ccggagagcg ggtggtggtc cgtgtgggca
     1741 ccgaggactc cggaggcgac ttgctggtga tcgacgggta cgtcaagcca ggcggcgccg
     1801 tcacgggaga gcacgtgcat ccgtccatcg acgaggactt cacggtgttg cgcgggcgcg
     1861 tgggcttccg tatcgatggt cttgagtcgg tcgcggagcc ggggacgtgc ctgcacgtgc
     1921 cggccggaac agcgcacgac tggtggaacg cgggcgacga agaggcgcac gtcagggtcg
     1981 agatccggcc cggcgcccgc ttcgaggaga tggccatcaa cctgtttggt ctggcccagg
     2041 acggcaagac gaactccagg ggcatgccta acctcctgca ggcggcgatc ctcgcccgtg
     2101 agttcagcga cgtgctgtac ttcaccagac cccccttgtg ggtgcagcgc ctgctgttcg
     2161 gctccctggc gtggctggcc cgccttctcg gctacagggg cagctacccc gggtatctgg
     2221 agcgcggccc caaggagcgg gtcgaggtgg agctgtggac gagccaggtt tgatggcaag
     2281 accagcatcg gcagcccgag agccggaaat cgtcgtccac atccggggcg gggatactgc
     2341 cggcaggtgc gggatggtgg agacggtcga gaagcggggt actcggtctc ctcgccacct
     2401 acatctccgg gaagatgaga cgctatacgt cgtagaaggc ttcctgagcc tctgggtcgc
     2461 gggcgagtgg gtcgaagccc cggcgagcac ggcgcacttc ctgccccgcg gggtggaaca
     2521 cgcccttctc gccgagacga cgacggcgag ggtgctctcc ttcttcgcgc ccgcgggttt
     2581 cgaagggttc tacggggaga tggccgccgc gggaccgtac gacatcgagc gcctggtgac
     2641 gacggcggcc aggtacggtt gtgagatagc cgggcccacc cccgaacggc cccggttgga
     2701 cccagcagcc agccgcccta ggcgtctccc tcggagaggg ccatcgcgca gttgaccagg
     2761 gcgaggtggg tgaacgcttg cgggaagttg ccgacgaacc tgttggcgcg ggtgtcgtat
     2821 tcctcgctca ggagaccgac gtcgttgcgc agggagagca ggcgttcgaa cagttcctcg
     2881 gcctccgcct tccggccgat gcccctcagc gcgtccacca gccagaagct acagatcagg
     2941 aaagatccct ccctgcccaa gaggccgtcg tctgactcgt ccgtacggta gcgcaggacg
     3001 aaaccgtcct cggtcagctc gtccctgatg gtctcgacgg tacttaccac gcgctcgtcg
     3061 tggtagggca agaaccccac ccgcgggatg aggagcgcgc aggcgtcgag cacgtcggag
     3121 tcgtacgcct gggtgaacga gcctttttcc tcgctgaagc ctctgtccat gacgtcggcg
     3181 tggatctcgt cgcgtacggc gcgccagcgg ttcacgggac cttcgagccc gaagcgctcg
     3241 acgcccttga ccagcgagtc gaacgctacc cagcacatca ccttggagta gacgaagtgc
     3301 tgcttctccc cgcgggactc ccagatgccg ttatccggc
//
LOCUS       DBNGVD010000137         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2214222, whole genome shotgun sequence.
ACCESSION   DBNGVD010000137 DBNGVD010000000
VERSION     DBNGVD010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2214222"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC16CM_03910"
     CDS             <1..318
                     /locus_tag="AC16CM_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR3139002.1"
                     /translation="SGKLIRLPLVMVVRDGKRYLVSMLGEDVNWVLNVKAANGRATLN
                     HGRSEDVRLEEVAPEHRAPVLRDYLGRAPRAGAHMPVQKDAPLADFERVSGRYPVFRV
                     VSV"
     gene            409..1260
                     /locus_tag="AC16CM_03915"
     CDS             409..1260
                     /locus_tag="AC16CM_03915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3139003.1"
                     /translation="MTDNVSNAAETLELGDLTVRRLGFGAMRLCGPGIWGVPEDPAAA
                     EAVLRRVVELGINLIDTADAYGPEVNERQIHSALYPYPEDLVIATKGGLVRPGPGRWN
                     RNGRPRHLREACEGSLRRLGVETIDVYQFHRPDSRVPFEDSVWALAELRDEGKIRHVG
                     LSNVGVREIAIALDIVPIVSVQNRYNLTDRSSEAVLETCEREGIAFIPWFPLATGDLA
                     RPGGDLDEIASSHGATPAQVALAWLLARSPVMLPIPGTSSVEHLEENVAATELELSEE
                     EIATLSS"
     gene            1318..2562
                     /locus_tag="AC16CM_03920"
     CDS             1318..2562
                     /locus_tag="AC16CM_03920"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3139004.1"
                     /translation="MPVSTTITTVYFRTALLAVLLVLLCAACGQTGGGNGGAEGGSGE
                     PEPIDVGETTAEATESGESSADEAGADETTAEASESEGDPTERVEVSTLATDLEVPWS
                     FAFLPGGDALVTERDSGRLLRVSVSGDVREVQTLPEGGSGEGGLLGVAVSPDYENDRY
                     VYAYYTTQRDNRVVRFRLGERPEPILTGIPVNTYHDGGRIQFGPDGMLYVSTGDAGDP
                     GNSQDRGSLGGKILRIEPDGSVPPDNPFAGSLVYSYGHRNVEGLAWDAEGRLYASEFG
                     QDTWDEVNRIEAGQNYGWPEVEGRGGEDEGYIDPLTVWPTSQASPSGAEIMVDGAVPQ
                     WEGDLFVAALRGERLWHLELNERGAITGRQKLLDGEVGRIRDVSQAPDGSLWVSTSNR
                     DSYGSPVSARDDRIFRLAPSGE"
     gene            2573..>3315
                     /locus_tag="AC16CM_03925"
     CDS             2573..>3315
                     /locus_tag="AC16CM_03925"
                     /inference="COORDINATES: protein motif:HMM:NF044863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sensor domain DACNV-containing protein"
                     /protein_id="HIR3139005.1"
                     /translation="MQNIRRTGQDAYPADLARFLCGVWDEERAGPLPEESKLEMLISA
                     CYQASLLHEEERAVTFRVILCDPARLPSSEGPPTGVHKLEFPEPRPFEVQELRRLSPA
                     ADYYRSLIGVYADREGGLKIWGLVHSGPRWLREGQGGRDAPPPLPPALVLRVYGPGRI
                     AADAGQENICKLEEGRISDLSMDVFRSRWLPAAFAPVRQELAEIHDTLRDQARKKGQV
                     WARLDNDITRVIGQHTIKRITSAVRDSHHG"
BASE COUNT          598 a         1012 c         1166 g          539 t
ORIGIN      
        1 tcgggtaagc tgatccgact gccactggtg atggtggttc gcgatggtaa acgctatctc
       61 gtttccatgc tcggggagga cgtaaactgg gtactcaacg tgaaggcggc gaatggcagg
      121 gcaaccctga atcacggtcg aagcgaagac gtgaggttgg aggaggtggc cccggagcac
      181 cgtgcgccgg tacttcggga ttacctgggg cgggctcccc gggccggggc gcacatgccg
      241 gtccagaagg acgccccgct cgccgacttc gagcgggtgt cgggcaggta ccccgtgttc
      301 agggtggtat cggtctgacg gtgtgaacat gtaggggccg gatccgacgg ccgtgccgga
      361 tctgaccgct gtggccctcg atagcgtgga cgacggaagg gacggcagat gacagacaac
      421 gtcagcaacg cggccgaaac gctcgagttg ggcgacctca cggtcaggcg cctcgggttc
      481 ggggccatgc ggttgtgcgg gcccggcatc tggggggtgc cggaggaccc cgcggccgcg
      541 gaggccgtgt tgaggagggt cgtcgagttg gggatcaacc tcatcgacac cgccgacgcc
      601 tacggccccg aggtgaacga gcgtcagatc cactccgccc tctaccccta cccggaggat
      661 cttgtaatag ccaccaaggg tggcctcgtc cgtcccggac cggggcgctg gaaccggaac
      721 ggtcggccgc ggcacctgcg cgaggcgtgt gagggaagcc tgcgccgtct cggtgtcgag
      781 accatcgacg tttaccagtt ccataggccg gacagccgcg tacctttcga ggactccgtc
      841 tgggccctcg ccgagctgcg ggacgagggc aagatccggc acgtgggcct atccaacgtc
      901 ggcgtgaggg agatagcgat agcgctcgac atcgtgccga tcgtcagcgt ccagaaccgg
      961 tataacctga ccgatcgctc ttcggaggcg gtccttgaga cctgcgaaag ggagggtata
     1021 gcgtttatcc cctggttccc gctcgcgacg ggcgaccttg cccgcccagg cggcgacctg
     1081 gacgagatag cgagtagcca cggcgctaca cccgcgcagg ttgccctggc ctggctcctg
     1141 gccaggtccc ccgtgatgct tccgatccca ggcacctctt cggtcgagca cctggaagag
     1201 aacgtcgccg caacagagct agagctctcc gaagaagaga tcgccacgct ctcgagttag
     1261 agtcggaatc gaggttcggc acaatataat ctccgccgaa tccgtagcag ggaggatatg
     1321 ccagtctcga ccactataac gaccgtgtac ttcaggaccg cgttgcttgc tgtcctgctc
     1381 gttctactgt gcgccgcttg cggacagacc ggtggtggaa acggtggcgc ggaaggcggc
     1441 agcggagagc cggagcccat cgatgtcgga gagacgacgg ccgaggctac cgagtccggt
     1501 gagtcctcgg cggatgaagc cggggccgac gagacgactg cggaggcttc agagtctgaa
     1561 ggggatccca cggagcgggt agaggtgagt accctggcca ccgacctcga ggtaccctgg
     1621 tctttcgcgt tcctacctgg tggcgacgcc ctcgttacgg agcgggattc cggcaggctc
     1681 ctcagggtga gcgtatccgg cgatgtcagg gaggttcaga ccctgcccga aggcgggtcg
     1741 ggcgagggtg ggctgctcgg cgtggcggtc tccccagatt acgagaacga ccgctacgtc
     1801 tacgcctact atacgaccca acgggataac cgggtggtga ggttccggct cggtgagagg
     1861 ccagagccca tactcacggg catcccggtc aacacgtacc acgacggggg caggatacag
     1921 ttcgggccag acggcatgct ctacgtgagc acgggcgatg ccggggatcc gggcaactcc
     1981 caggaccgcg gttctctcgg gggcaagatc ctgcgcatag agccggatgg ttccgttccc
     2041 ccggacaacc ctttcgcagg cagccttgtc tactcctacg gccaccgcaa cgtggagggt
     2101 ctggcctggg acgccgaggg gcggttgtac gccagcgagt tcgggcagga cacgtgggac
     2161 gaggtaaaca ggatcgaggc cggccagaac tacgggtggc ccgaggtcga gggccggggg
     2221 ggcgaggacg agggatacat cgatcctctc accgtctggc cgacctccca ggcctctccc
     2281 agcggggcgg agatcatggt cgacggagcc gtcccgcagt gggagggtga cctctttgtc
     2341 gccgccctca ggggcgagcg gctctggcac ctggagctga acgaacgggg ggcgatcacc
     2401 ggaagacaga agctcctcga cggggaggtg gggaggatca gggacgtgtc gcaggcgcct
     2461 gacgggtcgc tctgggtctc cacctccaat cgtgattcct acggcagccc ggtcagcgcg
     2521 cgggacgaca ggatctttcg gctagcccct tccggggaat agaatgagtg ccgtgcagaa
     2581 catcaggaga actggccagg acgcataccc ggcggatctc gcgcgcttct tgtgtggcgt
     2641 ctgggacgag gaacgggccg gaccgctgcc cgaagagagc aagttggaga tgctcatctc
     2701 cgcctgctac caggccagct tgctgcacga ggaggagcgg gccgttacgt tcagggtaat
     2761 cctgtgcgac cctgcgcgac tcccgtcgag cgagggcccg ccaacaggcg ttcacaagct
     2821 cgagttcccc gagccaaggc ctttcgaggt tcaggagctg aggcggctct cgcccgccgc
     2881 cgactactac cgctcactca taggcgtcta tgcggaccgg gaaggcgggc tgaagatctg
     2941 gggtctcgtt cattccgggc cgagatggtt gagggagggc cagggcggaa gggacgcccc
     3001 gcccccgttg ccgccggccc tcgtacttcg cgtgtacggt cccgggcgaa tcgcggccga
     3061 cgccggtcag gagaatatct gcaagctgga ggagggcagg atctccgacc tttccatgga
     3121 cgtgttcagg tcgcgctggc tgcccgctgc cttcgcgccc gtgaggcagg agctcgcgga
     3181 gatccacgac acgctcaggg accaggcacg caagaagggc caggtgtggg cccgtctcga
     3241 caacgacatc acgcgtgtga tcgggcagca caccataaag cgcatcacct ccgccgtcag
     3301 ggactcccat cacgg
//
LOCUS       DBNGVD010000138         3988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_574691, whole genome shotgun sequence.
ACCESSION   DBNGVD010000138 DBNGVD010000000
VERSION     DBNGVD010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_574691"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /gene="infB"
                     /locus_tag="AC16CM_03930"
     CDS             <1..1949
                     /gene="infB"
                     /locus_tag="AC16CM_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3139006.1"
                     /translation="AEQSGHNATIAQAPAEEPVSVPQSGRPQKKRRRVVIDASARNRG
                     ARPAAAPSTPREPERAPVEEEETKSSDGVRVEPGATVKDLGDALGVPPTQIIKVLMGL
                     GEMKTLTQTLSTEEIELIAEELGVQVEIGSVEEPEPVEIAPDDDPEDLVEKPPVVTVM
                     GHVDHGKTSLLDRIRRANVQSGEAGGITQHIGAYQVESEGRKVTFIDTPGHEAFTEMR
                     ARGARVTDVVVLVVAADDGIMPQTSEAIEHARAAGVPMVVAVNKIDVPNANQDRVFAE
                     LSERELVPEAYGGDTVTVPVSAKTGEGIEELLENILVVAELEDLKANPNAPASGYVIE
                     GERDPGRGPVATLLLSRGTLHKGDAVLAGTAYGRVRAMLDYTGQRVKDAGPGAPVEIL
                     GLSGVPEAGTRFEVLDHERIARDRAQQAEEKLRRQELAQGGSRRTLEELLGQGGTQDL
                     NLVIKADVAGSVEALKEALARLSTDEVRVNIVRSGVGALTDSDVMLGSASDGILLGFN
                     VRPTNTAKQVAEREGVEIRTYDVIYKALEEIEAAMRGMLAPEIQERETGTAEVRQTFR
                     VPNVGLVAGCYVTSGEIFRNNRVRVVRDGTVVYEGNIASLKRFRDDVRSVREGFECGV
                     GVENFNDVKDGDVLEFFETVEVPR"
     gene            1971..2240
                     /locus_tag="AC16CM_03935"
     CDS             1971..2240
                     /locus_tag="AC16CM_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3139007.1"
                     /translation="MRLELEMPFSSSLKDKRQILRSLKDRLRRKNVSVVESENQDSWQ
                     RATVEIALAAVSRGAAEERREELRRMLLGYEELAILDWREEFVKL"
     gene            2251..2607
                     /gene="rbfA"
                     /locus_tag="AC16CM_03940"
     CDS             2251..2607
                     /gene="rbfA"
                     /locus_tag="AC16CM_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3139008.1"
                     /translation="MSDRTRKVESQLKEITGEQVAALSDPRIQGLVTVTGVRVSPDLA
                     HATVFYSVLAGEDADAAHEGLQSAAGRIQAAVGAQTRLKRTPRLHFERDPVVESATRI
                     EAALREVRTDDDTHEE"
     gene            2585..3547
                     /locus_tag="AC16CM_03945"
     CDS             2585..3547
                     /locus_tag="AC16CM_03945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3139009.1"
                     /translation="MTTRTKNNTLAEIADFLKTLDRVAVTTHVGADGDAIGASAALVR
                     LMHKLGAEAVFCHSEAVQDYLAWLLPEEALTELPPGHDLLVGDTSRADRTGVPIPEAG
                     ARLNIDHHEDNPLYAEYNLVNPRAAATAEMVADLYAELGVPIDKPTAEALYVGLKTDT
                     GGFNFRNISPRAHELVADLLRAGVVPAEVNERINRRGNVEQLRIVGVSLANAVQYGEA
                     LISTVENADYERTGATEVESKESIDQLRSVAGVDVVAHLREVPQGTKGSLRSETVDVG
                     EVARHFGGGGHRLAAGYTTPRRPNEAREELLEVLKGVVDLGGER"
     gene            3613..>3988
                     /gene="truB"
                     /locus_tag="AC16CM_03950"
     CDS             3613..>3988
                     /gene="truB"
                     /locus_tag="AC16CM_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013660.7"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3139010.1"
                     /translation="MARVKHLLPKKTKIGHTGTLDPLASGLLVLLIGRSTRLSRYVMG
                     LRKSYTATARLGAVSDTLDAEGEISYLDAPIPSEDTLCEALPALTGDILQTPPMVSAL
                     KREGQRLYDLHRRGITVEREPRP"
BASE COUNT          738 a         1310 c         1367 g          573 t
ORIGIN      
        1 aggcggagca gtccggccac aacgccacca tagcccaggc cccagctgaa gagccggtct
       61 cggtgccgca gtccggccgg ccgcagaaga agcgccgccg agtcgtgatc gacgccagcg
      121 ccaggaaccg tggggcccgc cccgcggcgg cgccgtcgac cccgagggag cccgagcggg
      181 cccccgtcga ggaggaagag accaagagca gcgatggcgt acgcgtggag ccgggtgcca
      241 ccgtcaagga cctcggagac gctctagggg ttcccccgac ccagatcatc aaggtcctga
      301 tgggcctggg cgagatgaag accctgaccc agaccctctc caccgaggag atcgagctga
      361 tcgccgagga gcttggcgtt caggttgaga tcggctcggt cgaggagcct gagccggtcg
      421 agatcgcacc agacgacgac ccggaagacc ttgtcgagaa gccgcccgtg gtgacggtga
      481 tgggccacgt cgaccacggc aagacgagcc tgctcgacag gatccggcgc gccaacgtcc
      541 agtccggcga ggcaggcggc atcacccagc acatcggcgc ctaccaggtt gagtccgaag
      601 gccggaaggt gacgttcata gatacccccg gtcacgaggc gttcaccgag atgcgtgccc
      661 gtggagcccg cgtgaccgac gtggtcgtgc tggtggtcgc cgccgatgac ggcatcatgc
      721 cccagacctc ggaggccatc gaacacgcca gggccgcagg ggtacccatg gtcgtggcgg
      781 taaacaagat cgacgttccg aacgccaacc aggatagggt gttcgcggag ctctccgagc
      841 gggagcttgt gcccgaagct tacggcggcg acacggttac cgtgcccgtc tcggccaaga
      901 ccggcgaggg catcgaggag cttcttgaga acatcctggt cgtggcggaa ctggaagacc
      961 tcaaggccaa tccgaacgcg cccgcgagcg gctacgttat cgagggtgag agggacccag
     1021 gacgcggccc ggtggccacg ctgctcctta gccgcggcac gctccacaag ggcgatgccg
     1081 tgctcgcggg tacggcctac ggaagggtcc gcgccatgct cgactatacc gggcaacggg
     1141 tcaaggatgc cgggccggga gctcccgtgg agattcttgg cctctccggg gtcccggagg
     1201 ctggcacgcg cttcgaggtc ctggaccacg agcgcatagc ccgcgaccgc gcccagcagg
     1261 ccgaggagaa gttgcgccgg caggaactcg cccagggtgg gtcccgccgc acgcttgagg
     1321 aactgctcgg tcagggcggc acccaggacc tgaacctcgt tatcaaggcg gatgtggccg
     1381 gttcggttga ggcgctcaag gaggcgctcg ccaggctgtc taccgacgag gtgcgcgtca
     1441 acatcgtgcg tagcggcgtc ggggccctca cggactcgga cgttatgctc ggcagcgcct
     1501 ccgacgggat tctgctcggc ttcaacgtca ggcccaccaa cacagccaag caggtcgccg
     1561 agcgcgaggg cgtggagatt cggacctacg acgtgatcta caaggcgctc gaggagatcg
     1621 aagctgccat gcgcggcatg ctagcccccg agatacagga gcgcgagacg ggcacggctg
     1681 aggtgcgcca gaccttccgc gtgccgaacg tcggcctggt agccggctgt tacgtgacga
     1741 gcggtgagat cttccgcaac aaccgcgtcc gtgtggtgcg cgacggcacc gtggtctacg
     1801 agggcaacat cgcctccctc aagcgcttca gggacgatgt gcgctccgtg cgcgagggct
     1861 tcgagtgcgg cgtgggcgtc gagaacttca acgatgtcaa ggacggggac gtgctcgagt
     1921 tcttcgagac ggtagaggtc ccgaggtaaa ggcggctctt gttctgcgcg gtgaggctgg
     1981 aactggagat gcctttctcc tccagcctca aagataaacg acagattctg cgctccttga
     2041 aggaccgctt gcggcgcaaa aacgtctcgg tggtcgagtc ggagaaccag gactcctggc
     2101 aacgcgccac ggtggagatc gcgctcgccg cggtctcccg cggcgccgcc gaggagagac
     2161 gggaagagct ccgcaggatg ctgcttggct acgaggagct ggcgatcctg gactggcgcg
     2221 aggagttcgt gaagctgtga ggtcgaagcc atgagcgatc gcacccgcaa ggtcgagtca
     2281 caactgaagg agatcacggg cgaacaggtc gccgcgctat cggacccgcg catccagggt
     2341 ctggtaaccg taaccggtgt ccgcgtgagc cctgacctgg cccacgccac ggtcttctac
     2401 agcgttctgg ccggcgagga tgccgacgcg gcccacgagg gtctgcagag cgcggcgggc
     2461 cgcatccagg ccgccgtcgg agcccagacc cgtctcaagc ggacgccgcg cctgcacttc
     2521 gagcgggatc ctgtggtaga aagcgcgacc aggatagagg cagccctcag ggaggttagg
     2581 accgatgacg acacgcacga agaataatac gctggctgag atcgcggact tcttgaagac
     2641 cctcgaccgg gtggccgtga ccacccacgt cggggctgac ggggatgcga tcggggcctc
     2701 ggccgcgctg gtgcgcctga tgcacaagct cggcgccgag gccgtgttct gccactcgga
     2761 ggcggtacag gattaccttg cctggctcct tcccgaagag gcccttaccg agcttccccc
     2821 tggacacgac ctgctcgtag gggacacctc ccgcgccgac cgtaccggcg tgcccatacc
     2881 ggaggccggc gcccggctga acatcgatca ccacgaggac aacccgctct acgccgagta
     2941 caacctcgtc aacccgcggg ctgctgccac ggcggagatg gtagcggacc tctacgcgga
     3001 gcttggcgtg cccatcgaca agcccacggc cgaggcgctc tacgtgggcc tcaagaccga
     3061 cacgggtggc ttcaacttcc gcaacatctc gccgagggcc cacgagctgg tggcagatct
     3121 cctgcgcgcc ggcgtcgtgc ccgcagaggt gaacgagcgc atcaaccgca ggggcaatgt
     3181 agagcaactc aggatcgtgg gggtctccct ggccaacgcc gtgcagtatg gggaagccct
     3241 catctccacg gtcgagaacg cggactacga gcgcacgggc gccacggagg tggagtccaa
     3301 ggagtcgatc gaccagcttc gctcggtcgc tggcgttgac gtggtcgcgc acctgcggga
     3361 ggtcccgcag ggcacgaagg gctccctgcg ttcggagacc gtggacgtgg gcgaggtagc
     3421 ccgccacttc ggcggcgggg gccacaggct cgccgcaggc tacacaacgc cccgccggcc
     3481 caatgaggcc agggaagagc tcctcgaagt gctcaagggt gtcgtagacc tcggagggga
     3541 acgataaccg acgggcccct atcgggcggc ctgctgctgg acaagccgcc cggcatcacg
     3601 agcgcccgcg ctgtggcaag ggtcaagcac ctgctcccga agaagaccaa gatcgggcac
     3661 acgggcaccc tcgaccccct ggcctccggc ctgctggtgc tcctgatcgg ccgctccacc
     3721 cgcctctcgc gctacgtgat gggcttgcgg aagtcctaca cggcgacagc ccgactcggt
     3781 gccgtctccg acaccctgga tgcggaaggg gagatctcct acctcgatgc cccgatccca
     3841 tccgaagaca ccctgtgcga agcgctcccc gccctgacgg gcgacattct ccagaccccg
     3901 ccgatggtct ccgcgctgaa aagggaaggc cagaggctct acgacctgca ccgccggggc
     3961 attacggtgg agcgcgaacc ccgtcccg
//
LOCUS       DBNGVD010000139         5001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1121649, whole genome shotgun sequence.
ACCESSION   DBNGVD010000139 DBNGVD010000000
VERSION     DBNGVD010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1121649"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1109)
                     /gene="speB"
                     /locus_tag="AC16CM_03955"
     CDS             complement(156..1109)
                     /gene="speB"
                     /locus_tag="AC16CM_03955"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3139011.1"
                     /translation="MTRYEPADSFETPRFSGIRTFMRLPGNQDLGNADVAIVGAPFDT
                     GATFRAGARFGPEGIRSASALLRRYNPSLDVPIFDHLSVIDYGDVPVVPGYIDESYRR
                     IEEGLRPIHDAGVTPIVLGGDHSIALPELRAAAAVHGPLALVQFDSHPDTWDAYFGQN
                     HTHGTPFRRAVEEGLLDPSRSIQVGMRGSIYDKGDWDDAREMGFDLVPTDEVRNLGIP
                     EVIDRIRERVGDARTYVSFDVDFVDPAFAPGTGTPEIGGFTSREAQEFVRGLVGLEIV
                     GCDVVEVYPSYDPAQITALLAANIAHDLLSLIALRKGASSP"
     gene            complement(1134..2603)
                     /locus_tag="AC16CM_03960"
     CDS             complement(1134..2603)
                     /locus_tag="AC16CM_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3139012.1"
                     /translation="MAEAGAEQERSGGQQKLQPGLKRRHMTMISLGGIIGAGLFVGTG
                     PILNQAGPATILTYLLTGCLLILIMRMLGEMAVAQPSVGSFSDYGRMALGNWAGFSIG
                     WLYWYFWAIVVGFEATVAASIVGQYVSGIPPWLIALALVLLLTGTNLYSVGSYGEFEF
                     WFAGIKVAAIVIFIVLAILFLIGLFGPSPGLSNLYAKGGFFPNGPLAMFSGVATVIFA
                     FVGAEIVTIAAAESNEPGRNVARATNAVIYRVLLFYVVSVFFVAAIVPWNTGFTEDII
                     KSPFTLAFERIGIAGAGTVMNFVVLTAVLSVLNSSLYTTSRMLFALTRHNDAPRFLTN
                     TTRRGVPIWAILTGTIFAYVSVAIFYFFPEEIFTWLINASGAIALFVYLLIAVSQLVM
                     RRRLEREAPESLQLKMWFYPWLTYLSIAGIVAVLIGMAVLPDLRPLLIASLISLGVML
                     VAYFLRKAFGPPQRDPSEVIRESETRRESETRGESETTS"
     gene            complement(2709..3860)
                     /locus_tag="AC16CM_03965"
     CDS             complement(2709..3860)
                     /locus_tag="AC16CM_03965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3139013.1"
                     /translation="MIHFSAGELAARRRAASEELARRGLDGLLVFRQESMYYLTGYDT
                     FGYVFFQCLYLGADGETMTLLTRTPDVRQAEHTSLIGDIRVWYDAEGANPAEQLAVIL
                     EEHGCRGRRLGIETDAYGLTGFNMKRVEAALDGLCELVEASDLVTALRAVKSPAEIAY
                     VREAARLADLGLDRVVEVAGPGVFEGEVLAALQGAIFEGGGDLPANEVIIGSGPRALL
                     FRSAAGMRRMDAVDQLSLEWAGSYRHYHAAMMRTLAVGEANGYQRHLFSAVAEALEEM
                     TGALAPGRPVGEVDDAHRRVLDAAGLGEHRFAACGYSLGTTFSPNWMDWPMLFSGNAV
                     LAEPGMVFFLHCIVVDSEKGVAMSLGHTCLVTENGREVLSARPLGLTVV"
     gene            complement(3884..4993)
                     /gene="ald"
                     /locus_tag="AC16CM_03970"
     CDS             complement(3884..4993)
                     /gene="ald"
                     /locus_tag="AC16CM_03970"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3139014.1"
                     /translation="MGVPKEIKDMEGRVSMQPDGVAELVHHGHEVVVQSGAGKGAGFS
                     DEEYEEEGARLVDGPEEVFGAAGLIVKVKEPIPEEYDLFKEGQQLFTYLHLAADKQLT
                     EFLMDRKIESIAYETVETSDGLLPLLTPMSEVAGRMSVQAAAHHLESPQGGAGLLLGG
                     VPGTPAATVTIIGGGVVGTEAAKIALGMRALVSVLDISPKRLAYLSDIFEGRADLVIP
                     NRARTAAYVKQADVVIGAVLVHGAKAPKLVSREMVASMRPGSVVADVAIDQGGCIETA
                     RPTTHSEPTFVEEGVVHYCVANIPGAVARTSTLALTSATLPYLIQIAAKGIEGAAADN
                     EDLAKGLSTLRGDLVSGPVAEAHDLSYTDPSKILG"
BASE COUNT          889 a         1657 c         1610 g          845 t
ORIGIN      
        1 ctgtgccgcc gctccatcag ctcccggctc ttcggacccg gtatctcggt ctttatcctc
       61 gtcgacccaa gcatctcgcc tcctcttcct gtcggcatga tccgttccag tatactcgcg
      121 gcaacccgtc gggcgatacc cccggagggg gaccgttacg ggctcgaagc ccccttgcgg
      181 agcgcgataa ggctcagaag atcatgtgct atgttggccg ccaggagcgc cgtaatctgg
      241 gccgggtcgt aggatgggta gacctcgacc acgtcgcagc cgacgatctc gagccccacg
      301 aggccccgca cgaactcctg cgcctcccgg cttgtaaagc ccccgatctc gggcgttcca
      361 gtccccggcg cgaaggcggg atcgacgaaa tcgacgtcga acgaaacgta ggttctggcg
      421 tcgccgaccc gttcgcggat acggtctatg acctcgggta tgccgaggtt gcgcacctcg
      481 tcggtcggga cgaggtcgaa gcccatctcg cgcgcgtcat cccagtcgcc tttatcgtag
      541 atcgaacctc gcatgccgac ctggatggag cgggaggggt caagcaagcc ctcctcgacg
      601 gccctgcgga aaggcgtgcc gtgcgtgtgg ttctggccga agtaggcgtc ccaggtatcg
      661 gggtgggaat cgaactgcac gagggcgagc ggcccgtgaa ccgccgcggc ggccctcagc
      721 tccggcaggg ctatggagtg gtcgccgccg aggactatgg gcgtgacgcc ggcgtcgtgg
      781 atgggcctca ggccctcctc tatgcgtcgg tagctctcgt cgatgtagcc ggggaccacg
      841 ggaacgtcgc cgtagtcgat gacggagagg tggtcgaaga tgggcacgtc caggctcggg
      901 ttgtagcgcc tgaggagagc ggaggcgctg cggatgccct cggggccgaa gcgcgctccg
      961 gccctgaagg tcgcgcccgt gtcgaacggc gcgcccacga tcgcgacgtc ggcattcccg
     1021 aggtcctggt tgccaggcag gcgcatgaag gtgcggatgc ccgagaagcg cggcgtctcg
     1081 aaagagtctg ctggttcgta gcgggtcaat agtttttcac ctctatccgg gtatcaggag
     1141 gtcgtctccg actctcccct cgtctccgac tctctcctcg tctcggactc tctgatcacc
     1201 tcggaagggt ctctctgcgg ggggccgaac gccttgcgca agaagtaggc caccagcatc
     1261 acgccgaggc taatgaggct cgctatcaac aggggacgca ggtcggggag gacggccatc
     1321 ccgataagca cggccacgat gccagctatc gacaggtacg tcagccacgg atagaaccac
     1381 atctttagct gcagactttc gggcgcctcg cgctccaggc ggcggcgcat cacgagctgc
     1441 gagacggcga tcaagaggta gacgaagaga gcgatggccc ccgacgcgtt gatcagccac
     1501 gtgaatatct cttcggggaa gaagtagaag atcgcgacgg acacgtacgc gaagatggtc
     1561 ccggtaagga tagcccagat cggcacgcca cggcgggttg tgttggtcaa gaacctgggc
     1621 gcgtcgttgt gcctggtgag ggcgaaaagc atgcgggagg tcgtgtagag actggagttg
     1681 aggacggaga ggacggcggt caggaccacg aagttcataa ccgtccccgc ccccgctatc
     1741 ccgatcctct cgaaagcgag ggtgaacggg ctcttgatga tgtcctcggt gaacccggtg
     1801 ttccacggca cgatcgccgc gacgaagaac accgagacca catagaagag caggactctg
     1861 tagatcaccg cgtttgtagc cctggcgacg ttcctccctg gctcgtttga ctcagcggcg
     1921 gcgatggtga cgatctcggc cccgacgaag gcgaagatca cggtcgccac gccggagaac
     1981 atcgccagag gaccgttggg gaagaatccg cccttggcgt agaggttcga gagaccggga
     2041 gaaggtccga acaacccgat caggaagagt atggcgagta cgataaagat aacgatagcc
     2101 gcgactttga tgccagcgaa ccagaactcg aactcgccgt aggagccgac ggagtaaagg
     2161 ttggtgccgg tgagcagtaa gacgagggcc agagcgatga gccagggcgg tatgccggac
     2221 acgtactgcc ccacgatgga cgccgccacc gtggcttcga acccgaccac gatcgcccag
     2281 aagtaccaat agagccagcc tatggagaac ccggcccagt tgccgagggc catccggccg
     2341 tagtcggaga aggaccccac cgacggttgg gccaccgcca tctcccctag catgcgcatg
     2401 atcaggatga gcaggcaccc cgtcaggagg taggtcagta tggtcgccgg accggcctgg
     2461 ttgagtatgg gtccggtccc cacgaagagg ccggcaccga tgatcccgcc gagcgagatc
     2521 atggtcatgt gccgcctctt gaggccgggt tgcagcttct gctgcccgcc ggatctctct
     2581 tgctccgccc ccgcctccgc catgcatgcc ccttcccgga tcgtggtacc tccacgcgca
     2641 gcttacacgc taaacactct cttttacagc acccctgccg accgcccgtt tccggcgtgg
     2701 atacgccgct agaccacggt caaccccaac gggcgcgcgg agaggacttc cctcccgttc
     2761 tccgtcacca ggcaggtgtg gcccaggctc atggcgacgc ctttttcgga gtccacgact
     2821 atgcaatgca ggaagaagac catccccggc tcggccagta cggcgttccc cgaaaagagc
     2881 atgggccagt ccatccagtt ggggctgaag gtggtgccca gggaatagcc gcacgcggcg
     2941 aaccggtgct ccccgagccc cgccgcgtca agcacgcgcc ggtgggcgtc gtcgacctcc
     3001 cccaccggcc gtcccggcgc cagcgccccg gtcatctcct ccagggcctc cgcgaccgca
     3061 gagaagaggt gccgctgata cccgttggcc tcccccaccg cgagggtgcg catcatggcg
     3121 gcgtggtagt ggcggtagga gccggcccac tccagcgaca actgatcgac cgcgtccatg
     3181 cggcgcatgc cggcggcgga ccgaaacagg agggcgcggg gtcctgaacc tatgatcacc
     3241 tcgttcgccg gaaggtcccc gccgccctcg aagatggctc cctgcaacgc cgcgaggact
     3301 tcgccctcga aaaccccggg tcccgccact tccacaaccc ggtccaggcc aaggtcagca
     3361 agccgcgcgg cctcccggac gtatgcgatc tccgcgggtg acttgacggc ccgcaaggcg
     3421 gttacgaggt cggaggcctc gaccagctcg cagagaccat cgagggcggc ctccacccgc
     3481 ttcatgttga agcccgtgag gccgtaggcg tcggtctcta ttcccaggcg cctgcccctg
     3541 cagccgtgct cttcgaggat caccgcgagc tgctcggccg ggttcgcccc ctccgcgtcg
     3601 taccagaccc tgatgtcgcc gatcagggag gtatgttcag cctggcgtac gtctggggtg
     3661 cgggtcaaga gcgtcatggt ctcgccgtcc gcgcccaggt agaggcactg gaagaatacg
     3721 tacccgaacg tgtcgtagcc ggtcaggtag tacatcgact cctggcggaa cacgagcagc
     3781 ccgtcgaggc cacgcctggc gagctcttcg gacgcggccc gacggcgagc ggccagctcc
     3841 ccggcggaga agtggatcac gggccgcccg gctatcggat gctctacccg agtatcttgc
     3901 tcgggtcggt gtaggagagg tcgtgggcct cggccacggg cccggagacg aggtccccgc
     3961 gcaacgtgga cagacccttt gccaggtcct cgttgtcggc ggccgcgcct tcgataccct
     4021 tcgcggctat ctggatcagg tacggcaacg tcgcgctggt gagggcgagc gtcgaggttc
     4081 ttgccacggc cccagggatg ttggcgacgc agtagtgaac cacgccctcc tcgacgaagg
     4141 ttggctccga gtgcgtggtc ggcctcgccg tctctatgca cccgccctgg tcgatggcga
     4201 cgtcggcgac cacgctccca gggcgcatgc tcgcgaccat ctcgcgcgag acgagctttg
     4261 gcgcctttgc cccgtgcacg agcacggccc ctatgaccac gtcggcctgc ttgacgtagg
     4321 cggcggtcct ggcgcggttg ggtatgacca ggtcggccct tccctcgaag atgtcggaga
     4381 ggtaggcgag ccgcttgggg ctgatgtcca gcacgctcac cagcgcccgc atgccgaggg
     4441 ctatcttggc ggcctcggtg ccgaccacgc cgccgccgat gatcgtgacc gtcgccgcag
     4501 gcgtccccgg cacacccccg agcagtagcc cggccccgcc ctgcggactc tccaggtggt
     4561 gagcagcagc ctgtacggac atccttcccg caacctcgct catgggggta agaagcggca
     4621 agagcccgtc agaagtctcg accgtctcgt aggcgatgga ctctatcttg cggtccatca
     4681 agaactccgt gagctgtttg tccgccgcca ggtgcaggta ggtaaagagc tgctgaccct
     4741 ccttgaagag atcgtactcc tcagggattg gttccttgac cttcacgatc aggcccgccg
     4801 ccccgaacac ctcttcggga ccgtctacga gccgcgcgcc ttcctcctcg tactcctcgt
     4861 ccgagaaacc agcacccttg cctgcaccgg actggaccac cacctcatgg ccgtggtgca
     4921 cgagctcggc aaccccgtcg ggctgcatcg agacccggcc ctccatgtcc ttgatctctt
     4981 tgggtacgcc tattaccgaa g
//
LOCUS       DBNGVD010000140         1572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4764829, whole genome shotgun sequence.
ACCESSION   DBNGVD010000140 DBNGVD010000000
VERSION     DBNGVD010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4764829"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1163
                     /gene="nusA"
                     /locus_tag="AC16CM_03975"
     CDS             30..1163
                     /gene="nusA"
                     /locus_tag="AC16CM_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3139015.1"
                     /translation="MNTALLSALHEIETEKGIPFETVKGVLEESLLAAYEGREGAEED
                     ARVVLDEETGDLRVMKEGEDITPHDFTRIAAQVMRQTFYQRLSEVHSEQLMAEYGDRV
                     GKDVVTGIVQQAHRRMTLVDLGQVEAILPASEQVPNERYENGQRLKVFLLEIREGGRG
                     PSLTVSRRHEALLRNLFELEVPEIYDGLVEIKAVAREAGLRSKVAVWSKEAGVDPVGA
                     CVGPRGSRVRAVVSELRNEKIDIIQWDPEPARFIAKALSPARVREVYLDEDEKQAEVI
                     VPDDQLSLAIGREGQNARLAVKLTDWKIDIKPESQATEYEEEEEDWEPDEDSTMHRCR
                     AVLSNGRRCANMALPGSLFCGIQSHQAQAAEFEGMVEGRGSDE"
     gene            1156..>1572
                     /locus_tag="AC16CM_03980"
     CDS             1156..>1572
                     /locus_tag="AC16CM_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR3139016.1"
                     /translation="MSKRVYEIAREQSLENKEVLQRLQEAGVDVKSHSSSVDDNVYER
                     VFGDGSASNGRTEAQEAEAQEARAEAEQSGHNATIAQAPAEEPVSVPQSGRPQKKRRR
                     VVIDASARNRGARPTAEPSTPREPERAPVEEEETQSS"
BASE COUNT          335 a          457 c          568 g          212 t
ORIGIN      
        1 cgcgcgaacc tcaaggagga catatacaaa tgaatacggc attgttgtca gcgctacacg
       61 agatcgagac ggagaagggt atccccttcg agaccgtgaa gggcgtgctc gaagagtcac
      121 tgctcgccgc ctacgaggga cgtgaagggg ccgaagagga tgcccgggtg gttctggacg
      181 aggagaccgg cgacctcagg gtgatgaaag agggggagga tataacgccc cacgacttca
      241 cgcgcatagc ggcccaggtg atgcgtcaga cgttctacca gcgcctcagc gaggttcaca
      301 gcgagcagtt gatggccgag tacggggacc gggtcggcaa ggacgtcgta accggcattg
      361 tccagcaggc gcacaggcgc atgacgctgg tcgaccttgg ccaggtcgag gcgatcctgc
      421 cagccagcga gcaggtgccc aacgagcgtt acgagaacgg ccagcgcctc aaggtgttcc
      481 tccttgagat aagggagggt gggcgcggac ccagcctgac cgtgagccgg cgccacgaag
      541 ccctgctcag gaacctcttc gagctcgagg taccggagat ctatgacggg ctcgttgaga
      601 tcaaggctgt cgccagggag gccggtttgc ggtccaaggt ggccgtctgg tcaaaggagg
      661 ccggcgtcga ccccgtgggt gcgtgtgtcg ggccgcgggg tagccgcgtc agggccgtcg
      721 tctccgagct caggaacgag aagatagaca tcatccagtg ggaccccgag cccgcacggt
      781 ttatagccaa ggccttgagc cccgcgcggg tcagggaggt gtacctggat gaggacgaga
      841 agcaggcgga ggtgatcgtc cccgacgacc agctctcgct ggcgatcggg cgcgagggcc
      901 agaacgcccg cctcgccgtg aagcttacgg actggaagat agacatcaag cccgagagcc
      961 aggcgacgga gtacgaggaa gaggaagagg actgggagcc cgatgaggac tccacgatgc
     1021 accgctgcag ggcggttctc tccaacggcc gcaggtgcgc caacatggcg ctcccgggct
     1081 cgctcttctg cggtatccag tcgcaccagg cgcaagccgc agagttcgag ggcatggtag
     1141 agggtagggg atcggatgag taagagagtt tatgagatag cccgcgagca atcgctggag
     1201 aacaaggaag tgctacagcg cctgcaggag gcgggcgtgg acgtgaagag ccactcctcc
     1261 tctgtagacg acaatgtcta cgagagggtg ttcggggacg gtagcgcgtc aaacggccgc
     1321 accgaggccc aggaggcaga ggcgcaggaa gcccgggccg aggcggagca gtccggccac
     1381 aacgccacca tcgcccaggc ccccgctgag gagccggtct ccgtgccgca gtcaggccgc
     1441 ccgcagaaga agcgtcgccg cgtcgtgatc gacgcaagcg ccaggaaccg tggggcccgt
     1501 cccacggcgg agccgtcgac cccgagggag cccgagaggg cccccgtcga ggaggaagag
     1561 acccagagca gc
//
LOCUS       DBNGVD010000141         7672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2397239, whole genome shotgun sequence.
ACCESSION   DBNGVD010000141 DBNGVD010000000
VERSION     DBNGVD010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2397239"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..944
                     /locus_tag="AC16CM_03985"
     CDS             177..944
                     /locus_tag="AC16CM_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3139017.1"
                     /translation="MDYLEFLFLGVIGVAGGVLSGLVGVGGGAVFVPGLLFVGGWEIQ
                     EAVAASLVIIIFSSLSGTIRNARSNDPVNWPVAGLLSLAVAPSSLIGVLVSRVSPDVV
                     VEVAFASLLLALAYPTARGGGAYDGGRRISLPLVFLVGIFVGTVAGLVGVGGGVVLVP
                     LMVLGMGLTTKQAVSTSLAVVMFTGIVGAAGYVATGFRDPEQLLSLPPLIIGSVIGAP
                     LGVRMRDWLPERVVRIGFSVFMVVIALRLLSEAAGIF"
     gene            1056..1643
                     /gene="lepB"
                     /locus_tag="AC16CM_03990"
     CDS             1056..1643
                     /gene="lepB"
                     /locus_tag="AC16CM_03990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3139018.1"
                     /translation="MTERPYYEDQQFQTSEEERRPQRKKGGGALEYLVILLVSFALVF
                     GFVRPFVMEAFWIPSGSMIPTLEIGDRVLVNKFIYRFTEPDRGDIIVFESVDNPDEDL
                     IKRVVALPGDTVAVRRGHLILNGERQKEPFLNKNLPDRSFYAKTRVPKNHVFVMGDNR
                     GNSADSRVFGPLPEENIEGEAFLRFWPPNRIGILL"
     gene            complement(1946..2329)
                     /locus_tag="AC16CM_03995"
     CDS             complement(1946..2329)
                     /locus_tag="AC16CM_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3139019.1"
                     /translation="MSQRVADSFVEALWKLEEDRDVEALVEIHTEDCDVGNVAVPRTF
                     SGHEGLREFWTSYRDTFGDMKSEFRNVFADEDGHAALEWNTTGTANGNDVSYDGVSLL
                     EIEDGKVTRFRAYFDPRTVNEQVVQ"
     gene            complement(2399..2887)
                     /locus_tag="AC16CM_04000"
     CDS             complement(2399..2887)
                     /locus_tag="AC16CM_04000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3139020.1"
                     /translation="MGLKPGYEYYANLATEKGAVKIRLFAEEAPETVNNFVSLARDGY
                     FDGTTFHRVIEDFMAQGGDPTGTGMGGPGYTISDEFHPNLRHDRPGILSMANAGPNTG
                     GSQFFITYVATPWLDERHAVFGEVVEGMDVIGEIKERDPQRDRAPGDTIERVEIEELP
                     RS"
     gene            complement(2966..3562)
                     /locus_tag="AC16CM_04005"
     CDS             complement(2966..3562)
                     /locus_tag="AC16CM_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3139021.1"
                     /translation="MPRAGLSRVAVVEAAGALADEGGLENLSLVDLAGRLGIRKPSLY
                     NHVAGIGDLRRELALVGLRGLGRRLSSAAAGKAGEEGLFALAEAYRTFVKQRPGLYEA
                     TVRSYRLSDPDNADLAAAEREGMEPVLAVLASCGIHDREEAIHAARGLRSVAHGFATL
                     EEAGGFGMSVDQDESFSRLIGAYATGLLAYRDRPPGIG"
     gene            complement(3565..3990)
                     /locus_tag="AC16CM_04010"
     CDS             complement(3565..3990)
                     /locus_tag="AC16CM_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HIR3139022.1"
                     /translation="MGNRLGRESGESRVARDGASWSVLLARPAAMLRVEGATMLVGSV
                     LLYWVGGGSWWLFALLLLAPDLSMLGYLVGPRVGALTYNVFHSYPLPAALGIFGVLAG
                     APLAVSVALVWFAHIGMDRLVGYGLKYPTAFKDTHLQRV"
     gene            complement(4311..6398)
                     /locus_tag="AC16CM_04015"
     CDS             complement(4311..6398)
                     /locus_tag="AC16CM_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139023.1"
                     /translation="MLGKRLRRYLFALVLGAFALTAIPAAGFGQADRLQNLEPGERAE
                     LDEKVPVNFVFVGYDRDDVDADAFLAGLPDRYKPVVRSEYFYNESIDKSLLGLNYTYD
                     YNLKFAGGDYESKVFGFLSDAANPAPLTDYQKLYNGNSSAFCNPPDGDPLPCQTGGVR
                     DIEKNYSISAPAFEKWLAENPPEGVDTKRNTVFFVNWWGDGQQPREGFKHHVYTKTNE
                     PDPDTGFNFGREAASRKLIAWGGTTATDEENGLGSTRRVWFHDLSAGPDSYTDNWNVD
                     DPDLTGDGVEDYRLPPVWEYFAPGGYRPASGLTGDLSKVSRYVAIDLLFTSSPLYPPQ
                     FTPRLLPSKINLDVNTVEGISGTNASVRYQTPSLMIDEIRELHRLPYSIDQQDLAYDN
                     DARRCYTNWLRGVPCFEGKYPAYSGDANLFLYGVENLNRLLDDEQSRREYEATFINWA
                     VEDGANFQTSPPLLGRADDNYRTGVQSFIFSFLSPDIAQAYGLTTSEIHEYGHHLNLS
                     HPFDGFDYEEETDFGWDGEYYFTGVGNENNTMMSYIDLNWDFSQFDRDNTDRFQAAAY
                     INNANALTERIRKDPDAGRAADDLAAADASYGAAKAAIARHDYAATFRNSRAAYEHVL
                     KGARDAGVPVKESNEGRTAIPRATGNSEIPDRYIDKVPRSEQDGPLKRQKAEDIRVND
                     PRDFSPLAHRLRQ"
     gene            complement(6583..>7672)
                     /locus_tag="AC16CM_04020"
     CDS             complement(6583..>7672)
                     /locus_tag="AC16CM_04020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3139024.1"
                     /translation="TLIWSTRLVKGRGMAINERLLKRLIETPGVPGREEKQREIAREE
                     LGALTDEVRTDALGSVIGTKRGSGGARVMVAAHTDEIGYLVKYIDDKGFLRLQTLGGH
                     DPANMVSQRVLVTTSEGETLRGALQPARKPPHVATEEDKKTPKLDEFFVDLGIDAETV
                     KEKVRIGDYVVMDRTLEKVGDNLMGKAMDDRIGVFVLYEALRALGDHTATIFAAATSQ
                     EEVGLRGAEASGSALEPTVVVALDVTLALDVPGGGNENTITELGKGVAIKIMDGYSIS
                     NPKLVEHLRTIAERENIPYQMEILPAGGTDAGGVQRLHGGIPAVTLSVPCRYVHTPNE
                     TVSEADVEAAVKLLARYLEEDLDAGYDL"
BASE COUNT         1223 a         2495 c         2452 g         1502 t
ORIGIN      
        1 tcggttcgtt acggggcgcc atgtatgggg cttccctcca gtggcggtac actgtgcgcg
       61 gaaagtccgt gcgcagcggg atctagctga cagcgagctc taggcggagc cgggcgaagc
      121 gtgctgagga gcgaccgaag ggagcgtgct gcgaaggccg aaggccggag cgtgctgtgg
      181 attatctgga atttctgttt ctaggggtta tcggggtcgc cgggggggtg ctctctgggc
      241 tcgtcggggt tggcggcggg gcggtgttcg tgcctgggct cctgttcgtg ggaggatggg
      301 agatacagga ggccgtcgcg gcgagcctgg tcatcatcat cttcagctcc ctctcgggca
      361 ccatccgcaa cgcaaggagc aacgatccgg tgaactggcc ggtggccggc ctgctgtccc
      421 tggcggtcgc gccctcgtcc ctgatcgggg tcctcgttag cagggtctcc cccgatgtgg
      481 tcgttgaggt tgccttcgcc tcgctgctgc tggccctcgc ctacccgacc gcccgcggcg
      541 ggggtgcata cgacggcggc aggcgtatct ccctccccct ggtcttcctg gttggcatct
      601 tcgtcgggac ggtcgcgggg ctagtggggg tcggaggcgg ggtggtgctg gtgccgctca
      661 tggtgctcgg catgggcctc accaccaagc aggccgtctc caccagcctc gccgtcgtta
      721 tgttcaccgg catcgtgggg gccgccggct acgtcgccac cggcttcagg gacccggagc
      781 aattgctcag cctgccaccc ctcatcatcg ggtccgtgat cggcgccccg ctcggtgtcc
      841 gcatgaggga ctggctgccc gagcgcgtgg ttaggatcgg cttcagcgtg ttcatggtgg
      901 tcatcgccct ccgcctgctt tccgaggccg cgggtatctt ctagcctccc gcgccaaccg
      961 ttccggcacg gggccgggag aaaggtgcac gaagttgtgg ggggctgcga agttttccgg
     1021 gctatttggg tgctcgtgcg ttatgctaat gcctcatgac cgagcggccg tactacgagg
     1081 accagcaatt ccagacctcc gaggaggagc gccgtcccca acgcaagaag ggcggcgggg
     1141 cactcgagta cctggtcata ctgctcgtct ccttcgcgct cgtcttcggc ttcgtcaggc
     1201 cgttcgtcat ggaggcgttc tggatcccct cggggagcat gatccccacg ctcgagatag
     1261 gcgacagggt actggtgaac aagtttatct accgcttcac ggagcccgac cgcggggata
     1321 tcatcgtgtt cgagagcgtg gacaaccccg acgaagacct cataaaacgc gtggtcgccc
     1381 tgcccggtga caccgtggcg gtccgccggg gccacctcat cctgaacggc gaacgccaga
     1441 aggagccgtt cctgaacaag aacctcccgg acaggagctt ctacgccaag accagagtcc
     1501 ccaagaacca cgtcttcgtt atgggcgaca accgcggcaa ctcggccgac tcccgcgtct
     1561 tcggccccct gccagaggag aacatagaag gcgaggcctt cctccgcttc tggcccccaa
     1621 accgcatcgg catcctgctc tagagagcac gctccggcta acgcctacgc ttgtcagcac
     1681 actgcggccc ttcgggcctc cgtttgccag cacgcttcgc ccttgcaggg ctcgctttct
     1741 gctagatccc gccgcggcct gcaccttccg cacggggagt atcggtgcgt tggaaagcct
     1801 catgccggag ccgccgttac ggatcgagac gaccggccga aagcgaggcg aagccgaagc
     1861 gttgccgacg agcgagggcg ccagcccgag cgtgccgaca aggcgccttc aggcgccgtg
     1921 ccgacaagct gccgcaggca gcgtgctact ggacgacttg ctcgttcact gttcgcgggt
     1981 cgaagtaggc ccggaaacgg gttaccttgc cgtcttctat ctccaggagg ctaacgccgt
     2041 cgtaggagac gtcgttgccg ttggccgtgc ccgtggtgtt ccattccagg gcggcgtggc
     2101 catcctcgtc ggcgaacacg ttgcgaaact cgcttttcat gtcgccgaag gtgtcgcggt
     2161 agctcgtcca gaactcgcgt aggccttcgt ggcccgagaa ggtccggggc acggcgacgt
     2221 tgcccacgtc acagtcttct gtgtggatct ctaccagcgc ctcgacgtcc ctgtcttctt
     2281 ccagcttcca cagggcctcg acgaaactgt cagccactct ctgggacatg catcacctct
     2341 cgttagggta cgcgacgagg ttagtgctta cccttccggg gtgactgtaa atccgcactc
     2401 aggagcgcgg tagctcctcg atctccaccc tctctatggt gtcgcccggg gcgcggtcgc
     2461 gctgcgggtc gcgctccttt atctcgccga tcacgtccat gccctcgacc acctcgccga
     2521 agaccgcgtg cctttcgtcg agccatggcg tggccacgta ggtgatgaag aactgggagc
     2581 cgccggtgtt cggcccagcg ttcgccatgg agaggatacc gggcctgtcg tggcgcaggt
     2641 tggggtggaa ctcgtccgag atcgtgtagc cagggccgcc catccccgtg cccgtcgggt
     2701 cgccgccctg ggccatgaag tcctcgatca cgcggtgaaa ggtcgtgccg tcgaagtacc
     2761 cgtcgcgcgc cagggacacg aagttgttga ccgtctcggg cgcctcttcc gcgaagagcc
     2821 ggatcttgac cgcgcccttc tcggtggcga ggttggcgta gtactcgtag cccggcttga
     2881 gccccatctc gggcggcccg tcgtaggtct tgttcgccat cgcttgtcct tccgttggtg
     2941 ttcgccagaa cgtcgaacgc atatgctacc cgatacccgg cggacgatca cggtaagcta
     3001 gaagcccggt tgcgtatgcg ccgatcagcc tgctgaaact ctcgtcctga tccaccgaca
     3061 taccaaaccc tcctgcctct tccagggtgg cgaagccgtg ggcgacgctc ctgaggcccc
     3121 tggccgcgtg aatggcctct tcccggtcgt gtataccgca ggaagcgagc acggcgagga
     3181 ccggctccat tccctcacgc tcggccgccg cgagatcggc gttgtcgggg tcggagagcc
     3241 ggtaagagcg tacggtagcc tcgtagagcc ccggcctctg ctttacgaag gttctgtagg
     3301 cctccgcaag cgcgaagagc ccctcctcac ccgccttgcc ggcggctgca ctcgacagtc
     3361 tccggcccaa cccccgcaaa cccacgaggg ccagctcccg cctgaggtcc ccgatccccg
     3421 ccacgtggtt gtagagggag ggcttgcgga tgccaagacg cccggccagg tcgacgaggg
     3481 agaggttctc caaccctccc tcgtcggcca gagcgccagc cgcctccacg acggccaccc
     3541 tgctgagccc cgccctgggc acgctcagac cctctgcagg tgggtgtcct tgaaggccgt
     3601 ggggtacttc aacccgtagc cgacgagccg gtccatgccg atgtgggcga accacaccaa
     3661 cgctaccgag acggcaagcg gcgcccccgc tagcaccccg aagatgccga gcgccgccgg
     3721 aagcggatag gagtggaaga cgttgtaggt gagggccccg accctcgggc caacaaggta
     3781 tcccaacatc gagagatccg gggccagcaa gagcagcgcg aacagccacc aactcccccc
     3841 acctacccag tacaacaaca cgctcccaac caacatcgtc gccccctcga ccctcaacat
     3901 agccgccgga cgcgccagca aaacagacca cgaagcccca tccctcgcca cccgcgattc
     3961 ccctgactcc ctgcccaacc tgttccccat tttctgcacc tctcacgctc ggcgtgtttg
     4021 ctaacggtag tagttatata ctaacgctgt tagttttgca acccggcgtc cgggttcgcg
     4081 ttctcagggt tgctggatgt gcgtggggga ctgggatgtc cagcagaagg ggaagaggtc
     4141 accccgggct cgccggactg ggtatctcac cacaggttca ggctaccagc cctcagcaag
     4201 gacgatttgc tgagggctgg taattatagg cggtgagggt caacgctctg cggcgcgctt
     4261 tcgggccggc ggatgccctg cgccctcgaa aacgaggtcc tggggcggcg ctactggcgc
     4321 aggcggtggg ctagcggcga gaagtctcgc gggtcgttca cccggatgtc ctccgccttc
     4381 tgcctcttca gtggcccgtc ctgttcggag cgcggcacct tgtctatgta gcggtccgga
     4441 atctcgctgt ttccggtagc ccgggggatg gcggtcctgc cctcgttgct ctcttttacg
     4501 ggaacaccgg catcacgggc gcccttaagg acgtgctcgt aggccgcgcg ggagttcctg
     4561 aaggtggccg cgtagtcgtg cctggctatt gccgccttcg ccgcgccgta gctcgcgtcg
     4621 gccgcggcga ggtcgtctgc cgccctgccc gcgtcggggt ctttccggat cctttccgtc
     4681 agggcgttgg cgttgtttat gtaggccgcg gcctggaagc ggtcggtgtt gtccctgtcg
     4741 aactgggaga agtcccagtt caggtctata tagctcatca tggtgttgtt ctcgttgccg
     4801 accccggtga aatagtactc accgtcccag ccgaagtccg tttcttcctc gtagtcgaag
     4861 ccgtcgaacg ggtggctcag gttcaggtga tgcccgtact cgtggatctc cgaggtcgta
     4921 agcccgtagg cctgcgcgat gtccggcgag aggaagctga agatgaagct ctggacgccg
     4981 gtacggtagt tgtcgtcggc gcggccgagc aacggtggtg aggtctggaa gttcgccccg
     5041 tcttctacgg cccagttgat gaaggtcgcc tcgtactcgc gcctgctctg ctcgtcgtcc
     5101 aggagcctgt tcaggttctc caccccgtag aggaacaggt tggcgtcgcc cgagtaggcc
     5161 ggatatttgc cttcgaagca gggcacgccc ctgagccagt tcgtgtagca gcgtctggcg
     5221 tcgttatcgt aggccaggtc ctgctggtcg atgctgtagg ggagtcggtg gagctccctg
     5281 atctcgtcga tcatcaggga gggtgtctgg tagcgtacag aggcgttcgt gcccgagatg
     5341 ccctccacgg tgttcacgtc gaggttgatc ttgctgggca agagccgcgg cgtgaactgc
     5401 ggcgggtaga gcggggagct ggtgaacagc agatcgatgg cgacgtaacg cgagaccttt
     5461 gacaggtcgc cggtgagccc tgaggccgga cggtaaccgc ctggggcgaa gtactcccag
     5521 actggcggta gccggtaatc ctcgacgccg tcgcccgtga ggtccggatc gtccacgttc
     5581 cagttgtcgg tgtaggagtc tggcccggcg gagaggtcat ggaaccagac gcgcctggtc
     5641 gagccgaggc cgttctcctc gtcggtggcg gtggtgccac cccaggcgat gagcttgcgg
     5701 ctcgcggcct ccctgccgaa gttgaagccc gtgtcggggt ctggttcgtt ggtctttgtg
     5761 tagacgtggt gcttgaagcc ctcgcgcggt tgctgcccgt cgccccacca gttgacgaag
     5821 aacacggtgt tgcgcttcgt gtcgacgccc tccggcgggt tctcggccag ccacttctcg
     5881 aacgccggag cgcttatgga gtagttcttc tcgatatcgc gcacgccacc cgtctggcag
     5941 gggagggggt ccccatccgg cgggttgcag aacgccgacg agttgccgtt gtacagcttc
     6001 tggtagtccg tgaggggtgc cggatttgcc gcatcggaca ggaaaccgaa gaccttgctc
     6061 tcgtaatccc cgccggcgaa cttcaggttg tagtcgtagg tgtagttcag gccgagcagc
     6121 gacttgtcga tgctctcgtt gtagaaatac tcggagcgga ccacgggctt gtaccggtct
     6181 ggtagtccgg cgagaaacgc atccgcgtcc acgtcatcac gatcgtagcc cacgaacacg
     6241 aagttcacgg ggaccttctc gtccagctcc gcccgctctc ccggttccag gttctggagc
     6301 ctgtcggcct ggccgaaacc cgcggcgggg atcgcggtaa gcgcgaaagc gccaagaacc
     6361 agagcgaaca ggtaacgtct aagcctcttg cctaacatct tccctcccac cgacccgtgt
     6421 cacccgaatc ccaccccggc aagacgtgcc gcacaaacct acgcagaaca tcaaccagcg
     6481 tagtgtagcg gaccttgcaa gctgacgaaa cacactacag gcggccaacc ctcttctcta
     6541 gtcctggcta gtagctgaaa gccaacagct gatggctagc agctagagat catagccggc
     6601 gtcgaggtcc tcctccaggt agcgtgcgag gagcttaact gcggcctcca cgtcggcctc
     6661 gctgaccgtc tcgttcgggg tgtgcacgta gcggcagggg acggagaggg taacggcggg
     6721 gatgccgccg tgcaggcgct ggacgccgcc ggcgtcggta ccacccgccg ggagtatctc
     6781 catctggtat gggatgttct cgcgttcggc gatggtgcgc aggtgctcga ccagcttcgg
     6841 gttggagatg gagtatccgt ccatgatctt gatggccacg cccttgccga gctcggtgat
     6901 ggtgttttcg tttccaccgc cgggcacgtc caacgcgagc gtaacgtcga gcgccacaac
     6961 caccgtgggc tccagggccg agccgcttgc ctcggccccg cgcagtccga cctcctcctg
     7021 cgaggtcgcg gcggcgaaga tggtggccgt gtgatccccc agagccctga gtgcctcgta
     7081 cagcacgaag acgccaatgc ggtcatccat cgccttgccc atgaggttgt ccccgacctt
     7141 ctccagggta cggtccatga ccacgtagtc cccgatccgg accttctcct tgaccgtttc
     7201 cgcgtcgatg ccgaggtcaa cgaagaactc gtcgagcttg ggcgtcttct tgtcttcctc
     7261 ggtggccacg tgcggtggct tgcgtgcggg ctgcagcgcc ccacgcaggg tctcgccctc
     7321 cgatgtcgtg acgaggaccc gctgggagac catgttcgcc gggtcgtggc cgccgagcgt
     7381 ctgtagccgc aggaagccct tgtcatcgat atatttgacc aggtagccga tctcgtcggt
     7441 gtgggcagcg accatcacac gggccccgcc gcttccccgc ttcgtcccga tcacgctccc
     7501 cagcgcgtcg gttctgacct cgtccgtgag ggcgccaagc tcctcgcggg cgatctcccg
     7561 ctgcttctcc tcacgtcccg gtacgccggg cgtctcgatc agacgcttca aaagcctctc
     7621 gttgattgcc attccccgtc ctttcactaa cctcgtggac cagatgagcg ta
//
LOCUS       DBNGVD010000142         4046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3672965, whole genome shotgun sequence.
ACCESSION   DBNGVD010000142 DBNGVD010000000
VERSION     DBNGVD010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3672965"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1136)
                     /locus_tag="AC16CM_04025"
     CDS             complement(282..1136)
                     /locus_tag="AC16CM_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139025.1"
                     /translation="MKPRDALESDGGGIAEGRAEEAEERAALEPVGGTPGGFRNLVGK
                     ENSEWTAGPSLMAHGIIWLVIVALISVAIAYIRGQMEPGYTPKDINDAGALMFFVLGA
                     VASVIAVVARTQGAIVGEKQLGTAAWVLSKPASRRAFVLAKLAVHFRWLLVVVLLFPA
                     VAFYGLLTVVSTLPPPPLLFLGGLAILALGLLFYLALSLALGTIFESRGPLAGSVFGF
                     MVAGFMIASYAPWLTAAFPWLFFQSGYYLVSRGEIPTYGLISIPATALWTVLFVFLAL
                     RGFERTEL"
     gene            complement(1126..2100)
                     /locus_tag="AC16CM_04030"
     CDS             complement(1126..2100)
                     /locus_tag="AC16CM_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139026.1"
                     /translation="MVETRGLTKTYGQVTALKALDLSVRKNSIFGFLGPNGAGKSTAM
                     KLLLGLVRPTSGSGTVFGNDIVSESFRIRARVGYLAQNPRFYDHMTARETLRFVARFF
                     YSGPESAIERRVEESLSLVGLSDRADRRIAGFSGGELQRLGLAQAQINFPDLLILDEP
                     AASLDPMGRRDVLEIMTRLRDEHDTTIFYSTHILDDVQRVSDSVAILDRGRLLAQAPI
                     DELLTVGGIGGADYEVLLDGESETVMEQASARVRSQGWVSSLDESSEPGGGRRWIVRI
                     TDQPAAEENLLRVILQTRGVNVTGFGRKRQSLEQAFFDQIEKGGEDEA"
     gene            2353..2499
                     /locus_tag="AC16CM_04035"
     CDS             2353..2499
                     /locus_tag="AC16CM_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139027.1"
                     /translation="MGGIGKKIEGAAKKAAKDKISGGKKGKDSSGSKDSVGKVESAIK
                     KRLK"
     gene            2687..>4046
                     /locus_tag="AC16CM_04040"
     CDS             2687..>4046
                     /locus_tag="AC16CM_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3139028.1"
                     /translation="MVAQLTNVSLAFPDKKVLENVSLTIYPGDRISLVGENGAGKTSL
                     FRILKGKLEPDSGEVSFARGTRIGYLEQDLADVDQDPDRTCMDAALEPFGPLIELERV
                     IERLSSRLAEPGGEAPSLLADLGEAQHRFETSGGYEFRSRTESTLAGLGLPEPLWGRR
                     VSELSAGQRVRLALARLLLEEHDLILFDEPTNHLDVSAREWLEGHLTGMDAAYLVASH
                     DRRFLDAVSSKVAHLDRGELTVYPGDYTAFRELREQAEEEGWRKHEKNQKLSRKLQRQ
                     AQDYQRWSYAGEKKKRGAADKGFVSHKAAKLMKRSLAARRRMEEAAENARTEKPFEKS
                     PVKIQFGSARARSLVRAENLIVGYSEEWPLCGELSFEVSAGDRLAVLGPNGSGKTALL
                     RTVLGELPALGGELRLSPSTNVGYFDQDTRSVPPDDTALQVVAKTGCDETLARTVMGR
                     MGV"
BASE COUNT          766 a         1270 c         1331 g          679 t
ORIGIN      
        1 ggcctgccgg ccccgagcgt ggatgtcggt gaggcggccg tcgccgcgcg caccgttgac
       61 gttggaaggc tgcaggtact ctagggctag tccgtttggg tgcaggatat gctggtcccc
      121 gcgggcaaag attacaccgg gaggatctcg ttgatgtacg accctgtggc cctgcagcga
      181 tgctcacggt ggggttctgg taacgccatt ccgcaacgcc tccgggccct ctggcggccc
      241 gagccaccgt tccacctcct tcgcgaagag gtgggtatct tctagagttc ggtgcgttcg
      301 aaaccgcgca gggctagaaa gacgaacagc acggtccaga gtgctgtggc ggggatggag
      361 atcaggccgt aggtggggat ctcgccccgg cttaccaggt agtatccgga ctggaagaac
      421 aaccatggga aggccgccgt caaccacggg gcgtagctgg cgatcatgaa ccccgcgacc
      481 atgaacccga aaacgctccc ggccagcggc ccgcggctct cgaagatcgt tccgagcgcc
      541 agcgagagcg cgaggtagaa gagcaggcca agcgccagaa tcgcaagccc ccccaggaag
      601 agcagcggcg gcgggggaag tgtggagacg accgtcaaca ggccgtagaa cgccacggcc
      661 gggaagagca gcacgaccac caggagccac cggaagtgga ccgcgagttt ggccaggacg
      721 aacgccctgc gcgaagcggg cttgctcagg acccatgccg cggtcccgag ctgtttctcg
      781 cctacgatcg cgccctgagt tctggcgacc acggcgatca ccgaggcaac cgccccgagg
      841 acgaagaaca tcagcgcccc ggcgtcgttt atgtccttgg gcgtgtatcc cggctccatc
      901 tggccccgga tataggcgat cgccaccgag atcagggcca ctatgaccaa ccagattatg
      961 ccgtgcgcca tcagcgaagg gcccgcggtc cactcggagt tctccttgcc gacgaggttc
     1021 cggaatcctc ccggggtacc cccgaccggt tcgagggcgg cccgctcttc ggcctcctct
     1081 gcgcggccct cggcgatccc gcctccgtct gactccagcg catccctagg cttcatcctc
     1141 gccgcccttc tcgatctggt cgaagaaagc ctgctcgagg ctctgtctct tgcggccgaa
     1201 gccggtgacg ttcacgcccc gcgtttgcag tatcacccgc aggaggttct cctcggcggc
     1261 aggctggtcc gtgatcctca cgatccagcg acgtccccca cccggctccg atgattcgtc
     1321 gagcgaggag acccagcctt gcgagcgaac acgtgcgctc gcttgctcca tgacggtctc
     1381 cgactcgcca tcgagtaaga cctcgtagtc tgcgccccct atcccgccga ccgtcaggag
     1441 ctcgtctatc ggggcctggg cgaggagcct gccgcggtcg aggatcgcaa ccgaatcgga
     1501 gacgcgctgg acgtcatcga ggatgtgggt cgagtagaag atcgtcgtgt cgtgctcgtc
     1561 gcgcagtcgc gtcatgatct ccagcacgtc gcgtcgcccc atcgggtcaa gcgaggccgc
     1621 tggttcgtcg aggatcagaa ggtcaggaaa gttaatctgg gcctgcgcga gccccagccg
     1681 ctgcagttcc ccgcccgaga agcccgcgat cctgcggtcg gcccggtcgg agagcccgac
     1741 gagcgagagc gactcctcga cgcgccgctc gatggccgac tccggtcccg agtaaaagaa
     1801 ccgcgccacg aaccgcagcg tctcgcgtgc cgtcatgtgg tcgtagaagc gtgggttctg
     1861 ggcgaggtag ccgaccctgg cgcggatccg gaaactctcc gagacgatat cgttgccgaa
     1921 gacggtgccg gagccggagg tcgggcgcac gagcccgagc aacagcttca tggccgttga
     1981 ctttcccgcc ccgttcggcc ccagaaaacc gaagatggag ttcttccgca cgctgaggtc
     2041 gagggccttc aacgccgtca cctggccgta ggtcttggtg aggcccctgg tctcgaccac
     2101 ggcggacgta ctcccgcctg gatgcgctcc cggaacagaa ttgtcgtttg tggggatcgt
     2161 catgggatct gagagtttat cgctgcgagc aacaatgcca cgtaaacccg ttacgtgccg
     2221 accttgcgag ggtttcggag cgtcgaagat ttgccgacca gctccctgtc ggacgctggc
     2281 cccaatggtt ggtcgcgtgc atcgatgggt aatgatgtga acagatgaac accccgacga
     2341 agggcggacg acatgggcgg cataggcaag aagatagaag gtgcggcgaa gaaggccgcc
     2401 aaggacaaga tatctggcgg gaagaagggc aaggactcaa gcggttcgaa agatagtgtg
     2461 ggcaaggtag agagcgccat caagaaacgc ttgaagtaga agggcggttc tctcccggtc
     2521 ccgcccggat cccgagctct tgctccgggt tgctagatag cgttctccct ccccagcaaa
     2581 cacaccctgg cgggtagata ggcgctggga tatacttgcc ggcatgtagc gggcttcctt
     2641 tgcaacaggt ttttcctcgt tcttctctag cgcaaaggac ccttccatgg tcgcccaact
     2701 caccaacgtc tcgttagctt tcccagacaa gaaggtactc gaaaatgttt ccctcaccat
     2761 ctatccgggg gataggatct cgctcgtcgg cgagaacggc gcggggaaga ctagcctgtt
     2821 tcggatcctc aaaggaaagc tcgagcccga ctcaggcgag gtttccttcg ccaggggcac
     2881 gcgcatcgga tacctcgaac aggatctcgc ggatgtggac caggaccccg accgcacctg
     2941 catggacgcg gcgctcgagc ctttcgggcc cctgatcgag ctcgagcggg ttatcgagcg
     3001 gctctcctcc aggcttgccg agcctggagg ggaggctccc agcttgctgg ccgatctcgg
     3061 cgaggcccag caccgtttcg agacctccgg cggctacgag ttcaggtcga ggaccgaatc
     3121 cacgctcgcg gggcttggtc tccccgaacc gctctggggg cgaagggtct ccgagctctc
     3181 cgcgggacag agggtacggc tcgcgctggc gaggttgctg ctggaggagc acgacctcat
     3241 cctgttcgac gagccgacca atcatctgga cgtttcggcc cgcgagtggc tcgaaggaca
     3301 cctcacgggg atggatgcgg cctatctcgt ggcctcccac gaccggcgct tcctcgatgc
     3361 cgtctcgagc aaggtggccc acctcgaccg cggcgagctc acagtctacc ctggcgacta
     3421 caccgcgttc agggaactgc gggagcaggc cgaagaagag ggatggcgca agcacgagaa
     3481 gaatcagaag ttgagcagga agctgcagcg gcaggcccag gactaccaga gatggtcgta
     3541 cgccggcgag aaaaagaagc gtggcgctgc cgacaagggc ttcgtcagcc acaaggcagc
     3601 gaagctcatg aagcgttcgc tcgccgcccg gcgcaggatg gaggaggccg cggagaacgc
     3661 cagaacggag aagcccttcg agaagagccc ggtcaagata cagttcgggt cagcaagggc
     3721 gcggagcctc gtgcgggccg aaaacctcat cgtcggctat tcggaggaat ggcctttgtg
     3781 cggggagctc tcgttcgagg tctcggcggg ggacaggctg gctgtccttg ggccgaacgg
     3841 ctcgggcaag acggcgctcc tgcgtaccgt gctgggagag ctcccggccc tcggcgggga
     3901 gctccgtctc tctccttcta ccaacgtggg ctacttcgac caggacacca ggagcgtgcc
     3961 gcccgacgat acggcgctgc aggtggtcgc gaagaccggg tgcgacgaga ccctcgcaag
     4021 gaccgtgatg ggcaggatgg gggtaa
//
LOCUS       DBNGVD010000143         2819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_210892, whole genome shotgun sequence.
ACCESSION   DBNGVD010000143 DBNGVD010000000
VERSION     DBNGVD010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_210892"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1124
                     /gene="fba"
                     /locus_tag="AC16CM_04045"
     CDS             96..1124
                     /gene="fba"
                     /locus_tag="AC16CM_04045"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432022.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR3139029.1"
                     /translation="MALVTMRQLLDEAASGGYGVGAFNVNNLEQVQAIMEAARETESP
                     VIIQASRGARKYANDRFLYHLMEAATEVYPEISVALHQDHGNSPETCKSAIDLGFTSV
                     MMDGSLMDDGKTPSDFDYNVRVTREVVEMAHEAGVTVEGELGTLGGIEDGVGSGEVSL
                     TDPDQAVEFVERTGVDALAVAIGTSHGAYKFTKEPDREVLAMHIIEEINERLPTTHLV
                     MHGSSSVPKELVDIINEYGGKIEPTWGVPVREIQEGIKHGVRKVNVDTDLRLAATGAI
                     RKAFAEDPSEFDPRYYLKPARAAMQEVVRARMEAFGQAGHAKDYDPISLPDMAARYRE
                     QGHTLKVK"
     gene            complement(1204..1785)
                     /locus_tag="AC16CM_04050"
     CDS             complement(1204..1785)
                     /locus_tag="AC16CM_04050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3139030.1"
                     /translation="MQSVCVFCGSSSGDDPAYAQVARSLGATLAESKVRLVFGGGHVG
                     LMGEISNAAIAAGGDVIGVIPKFLVERELAHERVTDLRIVGSMHERKALMADLSDAFI
                     ALPGGSGTLEEFFEVLTWAQLGEHAKPCGLLNVAGYYEPLISMFDHMVGTGFLSKADR
                     SLLLVEDDPDRLLERFDHYQPPSRARWMDRSKT"
     gene            complement(1790..2236)
                     /locus_tag="AC16CM_04055"
     CDS             complement(1790..2236)
                     /locus_tag="AC16CM_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3139031.1"
                     /translation="MTWLLLLPAAFIAGMAAPTQFAINSQLRQAVGGPVMAATISFLV
                     GTVILFAATLIVSRSIPDLGPTVSAPWWMYLGGLLGAFYVCASVVLTPRLGVATTIGL
                     FLAGQVVASIVIDNFGLFGTPVQAASIPRILGAVLIIVGVAVVQRF"
     gene            complement(2268..2525)
                     /locus_tag="AC16CM_04060"
     CDS             complement(2268..2525)
                     /locus_tag="AC16CM_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139032.1"
                     /translation="MEREPLSPESDALWRRLWEMWQDNDEEDVILDSSKLDDLEDEIP
                     GLENRMKTALAYLQRARYIQYRSGVGEDGIEPILYDVYEPR"
     gene            complement(2528..>2819)
                     /locus_tag="AC16CM_04065"
     CDS             complement(2528..>2819)
                     /locus_tag="AC16CM_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3139033.1"
                     /translation="VTTGGTGISGRDTTYEVVERMLEKRLDGFGEIFRMLSYEEIGSA
                     AIMSRALAGSVGGKFVASLPGSRNAVRLAVEKLLVPELAHIVFELRKHQESR"
BASE COUNT          513 a          871 c          947 g          488 t
ORIGIN      
        1 ttggcccgga gcgggaaagc gtctatactt gccgttcccg tcacggttga tggtggtggg
       61 gttctgtact tcgatagatt cgggagggtt gttttatggc actagtaact atgcgccagc
      121 ttctggatga ggcggccagt gggggatatg gcgttggggc gttcaacgtc aacaacctgg
      181 agcaggtgca ggcgatcatg gaggccgcgc gggagacgga gtcgccggtc atcattcagg
      241 cctcgcgggg ggcgcgaaag tacgccaacg accgctttct gtaccacctg atggaggccg
      301 ccaccgaggt gtacccggag atctccgtgg ccctgcacca ggaccatggc aactcgcccg
      361 agacctgcaa gagcgccata gacctcgggt tcacgtcggt gatgatggac ggctccctta
      421 tggacgacgg gaagacccct tcggatttcg attacaacgt gcgcgttacg cgcgaggtgg
      481 tcgagatggc ccacgaggcc ggcgtcaccg tggagggaga gctgggcacg ctcggcggca
      541 tcgaggacgg cgtcggatct ggcgaggtca gccttacgga cccggaccag gcggtcgagt
      601 tcgtggagcg gaccggggtg gacgccctgg cggtcgccat cgggacgagc cacggggcct
      661 acaagttcac caaggagccg gaccgcgagg tcctggccat gcacatcatc gaggagatca
      721 acgagcggct cccgaccacc cacctcgtca tgcacggctc ctccagcgtt cctaaagagc
      781 tggtggacat catcaacgag tacggcggga agattgagcc cacgtggggc gtgcctgtgc
      841 gcgagattca ggagggcatc aagcacggcg tgaggaaggt gaacgtcgat acggatctac
      901 gcctcgcggc gaccggcgct atccgcaagg ccttcgccga ggatccctcc gagttcgacc
      961 cccgctacta cctcaagccc gcccgcgccg cgatgcagga ggtcgtgagg gctcgcatgg
     1021 aggccttcgg gcaggccggc cacgcgaagg actacgaccc catctcgcta cccgatatgg
     1081 cggcccgtta cagggagcag ggccacacgc tcaaggtgaa gtagagctgt cagcaatcag
     1141 ccgtcagcgg tgggcttttt tattgggctg atcgctgacg gctaaccgcg gcgagcttcc
     1201 ggatcaggtc tttgatcggt ccatccacct ggctcggctg ggcggttggt agtggtcgaa
     1261 cctctcgagt aggcggtctg gatcatcttc tacgaggagg agggagcggt ccgccttgct
     1321 caggaagccc gttccaacca tgtgatcgaa catggagatg agcggctcgt agtagccggc
     1381 gacgtttagg agaccgcagg gcttcgcgtg ctcgcccagc tgggcccagg tcaggacctc
     1441 gaagaactcc tccagggttc cgctgccgcc gggaagcgcg ataaaagcat ccgagaggtc
     1501 ggccataagg gccttgcgct cgtgcatgga gccgacgata cgcaggtctg tgacgcgctc
     1561 gtgtgctagc tcccgctcga cgaggaactt cgggattacc ccgatcacgt cgccgccggc
     1621 cgcgatggcg gcgttcgaga tctcgcccat caggccgacg tgaccgcccc cgaagaccag
     1681 gcgtactttc gactcggcga gcgtggcgcc gagcgagcgg gccacctgcg cgtaagcggg
     1741 gtcatcaccg ctgctcgagc cgcaaaagac gcagacgctc tgcaaggcgc tagaacctct
     1801 ggacgacggc aacgcccacg ataatgagga ccgcccccag gatgcgcggg atgctcgccg
     1861 cctgtacggg ggttccgaac aggccgaagt tgtctatcac tatagaggct acgacctgcc
     1921 cggccaggaa caggccgatg gtggttgcca ccccgaggcg gggcgtgagc acgaccgagg
     1981 cacacacgta gaaggccccg agcagaccgc ccaggtacat ccaccagggg gcgctcacgg
     2041 tggggccgag gtccggtatc gagcggctca ctatcagggt ggcggcgaac aggatcaccg
     2101 tgccgaccag aaacgagatc gtggcggcca tcaccggccc gccgacggcc tggcgcagtt
     2161 ggctgttgat ggcgaactgc gtgggtgcgg ccatcccggc gatgaacgcg gcgggaagta
     2221 gcaggagcca ggtcatcttg ttcacgctct cctcgtcttt ggccgcatca acggggttcg
     2281 tacacgtcgt agagaatcgg ctcgatgccg tcctcaccga cgccggagcg gtactggatg
     2341 tagcgggccc gctggaggta ggccagcgcg gtcttcatcc tgttctcgag gccggggatc
     2401 tcatcctcga ggtcgtccag cttgctagag tcgaggatca cgtcctcctc gtcgttgtcc
     2461 tgccacatct cccacagcct gcgccagagt gcgtcggact cgggggacag gggttccctc
     2521 tccatctcta cctgctttcc tggtgtttgc gaagttcaaa tacgatgtgc gcgagctcgg
     2581 gcacgagtag cttctccacg gcgagcctga ccgcattgcg ggagcctgga aggctcgcga
     2641 cgaacttgcc gccaacgctc cccgcgagcg cccgactcat tatggcggcc gagccgatct
     2701 cttcgtagga gagcatcctg aagatctcgc cgaacccgtc gagcctcttc tcgagcatgc
     2761 gctccacgac ctcgtaggtg gtgtcgcggc ccgagatgcc ggtgccgccc gtcgtgacg
//
LOCUS       DBNGVD010000144         4070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_575577, whole genome shotgun sequence.
ACCESSION   DBNGVD010000144 DBNGVD010000000
VERSION     DBNGVD010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_575577"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16CM_04070"
     CDS             <1..573
                     /locus_tag="AC16CM_04070"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3139034.1"
                     /translation="VGEVAEPAGEGRGGSSTLPHKRNPILSVIATANTRRVQAHAQTL
                     YGSMAGEHERAAGAWHAEWETLSEALALTGGAAAAVRETLEGLEVYPERMRRNLDETG
                     VMLMAENVVTVVADRLGRLEAHEIVRTAARRAADGGKTFRDELLAEPALQDRLSPDEV
                     DAALDPAGYLGSSGGFIDRALILYHREVST"
     gene            570..977
                     /locus_tag="AC16CM_04075"
     CDS             570..977
                     /locus_tag="AC16CM_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3139035.1"
                     /translation="MTKDGAYERGMRARREVLGDDYVDASMEQTTPFSADFQDLITRY
                     AWGETWTRPGLDRKTRSCITLTALMARGLPEELGLHVSGALRNGLTEEEIKEVILHGA
                     IYCGVPAARAAFAVAQRAIAEYHESQRSGGENG"
     gene            1086..2303
                     /locus_tag="AC16CM_04080"
     CDS             1086..2303
                     /locus_tag="AC16CM_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HIR3139036.1"
                     /translation="MRTQVGIVGGGPAGTLLSHLLHLRGIESVVLELRSREEVETTVR
                     AGVLEDGTASLLRETGVADRMDREGAVHHGIELRFDGAGHRIDFTDLVGKSIVIYGQQ
                     EVVKDLYTARLEAGGRILFGAAAVGLQDLDTREPRIRFRRGARFDSEAGEIRFDGDAP
                     EEVLHCDFVVGADGFFGPCRSAIPANIRTEHSRTYPFGWFGILVEGPPSTEELIYALH
                     DRGFALVSTRSPEIQRLYFQCDPTDHVGNWSDGRIWDELQARLATSEADWTLQRGDIF
                     QKSIVQMRSFICEPMQHGRLFLAGDAAHIVPPTGAKGMNLAVSDVLVLSRGLAAFYTS
                     GDAELLDSYSQTCLRRVWKASRFSWWMTSMLHRFHDEDDFRYRLQLAELDYVTSSRAA
                     STSLAENYVGLPI"
     gene            complement(2382..3230)
                     /locus_tag="AC16CM_04085"
     CDS             complement(2382..3230)
                     /locus_tag="AC16CM_04085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3139037.1"
                     /translation="MSTVPDINLNDGNAIPQLGFGVFQIDPDETAEAVNEALKVGYRH
                     IDTAEMYGNEREVGEGIRASGLDRADVFITSKLNNSYHDPDDARRAFDETLSDLGYDY
                     VDLFLIHWPLPTLYDGDFVSTWQTMEEFKADGRARSIGVSNFQVDHLERLAAETETVP
                     AVNQIEVHPYFTNEAVRAYGQEHGIATEAWSPIAQGGVLEDSTITRIADNVGKTPAQV
                     VLRWHIQRGDIIFPKSVTPSRIQENFEVFDFELGSGDMEEISGLDQGEDGRTGPHPDT
                     FDKVPS"
     gene            3384..3457
                     /locus_tag="AC16CM_04090"
     tRNA            3384..3457
                     /locus_tag="AC16CM_04090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3418..3420,aa:Asp,seq:gtc)
     gene            3575..3913
                     /gene="trxA"
                     /locus_tag="AC16CM_04095"
     CDS             3575..3913
                     /gene="trxA"
                     /locus_tag="AC16CM_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3139038.1"
                     /translation="MSEPIAVTEDTFEDEVLKSEVPVLVDFWAAWCGPCRQVAPVMDQ
                     IAAEYDGSLKVAKVDVDAEQGLAGAFGVSSIPTIAFFEPGEQPKAVVGALPKEMVESA
                     FELDRFAEAG"
BASE COUNT          721 a         1288 c         1338 g          723 t
ORIGIN      
        1 gtgggggagg tcgccgaacc tgccggcgag ggccgtggtg gctcctcgac gctcccgcac
       61 aagcggaacc ccatcctctc cgtaatagct accgcgaaca cccgccgcgt tcaggcgcac
      121 gcgcagacgc tctacgggtc catggccggc gagcacgagc gcgcagccgg cgcctggcac
      181 gccgagtggg agacgctctc ggaggctctc gccctgacgg gcggcgccgc ggccgccgtg
      241 cgggagacgt tggaggggct ggaggtctat ccggagagga tgcgtcgaaa cctggacgag
      301 acgggggtga tgcttatggc tgagaacgtg gtgacggtcg tcgccgaccg gctcgggcgc
      361 ctggaggccc acgagatcgt gcggacggct gcccggcggg ctgcggatgg cggcaagacg
      421 ttccgggacg agctactggc ggagccggca ctgcaagatc ggctctcgcc ggatgaggtc
      481 gacgccgccc tcgacccggc aggctacctg ggttcgtcgg gagggtttat cgaccgcgcg
      541 ctgatcctct accacaggga ggtttccaca tgacgaagga tggggcctac gagcgtggca
      601 tgcgggcgcg cagggaggtt ctgggcgacg actacgtcga tgcctcgatg gagcaaacga
      661 cgccgttctc cgcagacttc caggacctga taacacgcta cgcgtggggc gagacctgga
      721 cgcgacccgg gctcgaccgc aagacccgta gctgtatcac cctgacagcg ctcatggcgc
      781 gggggctacc tgaagagttg ggcctgcatg tgagtggagc ccttcgcaac ggccttacgg
      841 aggaggagat caaagaggtc atcctgcacg gggctatcta ctgcggggta cccgccgcca
      901 gggccgcttt cgccgtcgcc cagcgtgcca tcgccgaata ccacgagagc cagaggtcgg
      961 gaggcgagaa cggttgagga agggccacac cgtcatcggc cggtctacgc ggtctccagg
     1021 tcccctgcag ggaacctgga gatccgtccc ggcgttcgtt atgccgcagt ggaaggtagg
     1081 ggacgatgcg cacacaggta ggtatcgtgg ggggcgggcc ggcggggacg ctgctatcgc
     1141 accttctgca cctgcggggc atagagtcgg tggtgctgga gctcagatcg cgagaggagg
     1201 tcgagaccac ggtcagggcc ggggtgctgg aagatggcac ggcgagcctg ctgcgcgaga
     1261 cgggcgtcgc cgaccggatg gaccgcgagg gggctgttca ccacgggatc gaactccgtt
     1321 tcgacggggc cgggcaccgc atcgacttca ccgacctcgt cggcaagtcg atcgtgatct
     1381 acggccagca ggaggtggtg aaggacctct acacggcccg gctagaagcc ggcggaagga
     1441 tcctcttcgg ggccgccgcg gtgggcctgc aagacctcga taccagagag ccgaggatca
     1501 gattccgacg cggcgcgagg tttgactccg aggctggaga gatacgtttc gatggtgacg
     1561 cgccggagga agtactgcac tgcgacttcg tggtaggcgc cgacggcttc ttcggaccct
     1621 gccgcagcgc catccccgcg aacatccgca cggagcactc ccgcacctat ccgttcgggt
     1681 ggttcggcat cctggtagaa ggtccgcctt ctacggagga gctcatatac gctctgcacg
     1741 accgcggttt cgcgcttgtg agcacccgct cgccggagat acagcgcctg tattttcagt
     1801 gcgatcccac cgaccacgtg ggcaactggt ccgacgggag gatctgggac gagctgcaag
     1861 cccggcttgc caccagcgaa gcggactgga cgctacagag gggcgacatc ttccagaaga
     1921 gcatcgtgca gatgcgctcc tttatctgtg agcccatgca acacggtcgc ctctttctgg
     1981 ccggggacgc ggcccatatc gttcccccga cgggtgctaa gggcatgaac ctcgccgtct
     2041 ccgacgtcct cgtgcttagc cgcggtctcg ccgctttcta cacttcgggc gacgcggagc
     2101 tccttgactc ctactcacag acgtgcctca ggcgcgtgtg gaaagctagc cgcttctcgt
     2161 ggtggatgac gtccatgctt caccgcttcc acgacgagga tgacttccgc taccgcctgc
     2221 aactcgccga actcgactac gtcacctcct cgcgggccgc ttccacctcc ctggccgaga
     2281 actacgtagg tctacccatc tagagatagc ccggaagcag agaagggacc ccgctgctct
     2341 ggcggggtcc cttctctcgc gtgaggctct gtgggaggta gttagctcgg taccttatcg
     2401 aaggtatcgg ggtgcggtcc ggtacgaccg tcttcgccct ggtcgagccc ggagatctcc
     2461 tccatgtcgc cggagcccag ctcgaagtcg aagacctcga agttctcctg gatgcgcgag
     2521 ggcgtcacgg acttggggaa gatgatatcg ccgcgctgga tgtgccagcg gagcacgacc
     2581 tgggcgggcg tcttgcccac gttatcggct atccgggtga tggtggaatc ctccagcacc
     2641 ccgccctggg cgatgggcga ccaggcctcg gtggcgatcc cgtgttcctg gccgtaggcc
     2701 cgcacggcct cgttggtgaa gtaggggtgg acctcgatct ggttcaccgc aggtaccgtt
     2761 tcggtctcgg ccgccaggcg ctccaggtgg tcgacctgga agttggagac gcctatggac
     2821 cgggcgcggc cgtcggcctt gaactcctcc atggtctgcc aggtggagac gaagtcgccg
     2881 tcgtagagcg tgggcagggg ccagtggatc aggaacaggt ctacataatc gtagccgaga
     2941 tcggagagag tctcgtcgaa ggctctcctc gcgtcatcgg ggtcgtggta gctgttgttt
     3001 agcttgctgg tgatgaagac gtcggcccgg tcgaggccag aggcgcggat tccctcgccg
     3061 acctcccgct cgttgccgta catctccgcc gtgtcgatgt gcctgtatcc gaccttgagg
     3121 gcctcgttta cggcctcagc ggtctcgtcc ggatctatct gaaaaacccc gaagccaagc
     3181 tgcgggatgg cgtttccatc gtttagatta atgtctggta ccgtgctcaa atctttacaa
     3241 ctccttctag ctcgcgtgca aactcctgaa ccgggagcca cacgtgagta cccccaacca
     3301 gccaccccca aacagcgccg cccacccgcc acgcacgtta gaaaaccaca gccccgtttg
     3361 ataaactcca gttcgccctg cgcgggggcg tagttcagtt ggttagaacg ccggcctgtc
     3421 acgccggagg ccgcgggttc gagtcccgtc gctcccgccc acacgatacc cacggcctca
     3481 cccctccccg cgaccccgaa accccaggag ccgttacccc taccttgaca cctacatata
     3541 ctagagaaca gatacaagta agaggaggca cagcatgtcc gagccaatag cggtcacgga
     3601 agacacattc gaagacgagg tattgaagtc cgaggtaccg gtgctagtag acttctgggc
     3661 tgcgtggtgc ggtccctgcc ggcaggtagc gcctgttatg gaccagatcg ccgccgagta
     3721 cgacggttct ctgaaggtgg ccaaggtcga cgtggacgcc gagcaggggc tcgctggggc
     3781 cttcggcgtc tccagcatcc caaccatcgc gttcttcgag cctggcgagc agcccaaggc
     3841 cgtcgtcggg gcgcttccca aagagatggt cgagtcggcc ttcgagctcg accgcttcgc
     3901 cgaagccggt taagcccgtc aagacaccac cccggccggg taccccacct ggccggacgc
     3961 ttgattgcgt gcatcccgta ccgtagaatc tctctctgcc gatcctccgg cgggatagct
     4021 cagttggtag agcacatgac tgaaaatcat ggtgtccccg gttcgaatcc
//
LOCUS       DBNGVD010000145         7622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4765513, whole genome shotgun sequence.
ACCESSION   DBNGVD010000145 DBNGVD010000000
VERSION     DBNGVD010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4765513"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16CM_04100"
     CDS             complement(<1..328)
                     /locus_tag="AC16CM_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139039.1"
                     /translation="MSGRLEGRRVLVTHAERYIGPPVVELFREEGADVIADQSDYTDP
                     ATIKSVVEEAGRIDVLVANFAGPPSMMPVTNMLTETTEFEDSDFEAYLQELVWPLLRF
                     VRAALPQ"
     gene            528..1937
                     /locus_tag="AC16CM_04105"
     CDS             528..1937
                     /locus_tag="AC16CM_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HIR3139040.1"
                     /translation="MKPLDPRLFAAILAAGSARLASRTLKTGGGSTMPGVVARRVDPK
                     VLTKLSRRLSGGSAAITGTNGKTTTTRMVSTILRSAGIRAVNNSTGANLVTGVTAALV
                     ADSGLSGTPSSEMGLFEVDEASVPRVAAEAEIRILAVLNLFRDQLDRYGELAYTGKVI
                     SSSFGDLPRDGAVVLNADDPLVASLGRSAGRPVFYGVDEPALDTGRLQHVADSKDCPL
                     CGTALDYGTVYMGHVGVYDCPRCDFSRPTPDYRASRVRLEGAGGSSFLLSTPKGEVEA
                     KVSLPGLYNVYNALAAAAVAGEAGVGLQEISRGLGEFGGAFGRVERVRAGDREAFLLL
                     IKNPVGFNEILRTFVTGADAKNVLIAINDNDADGRDVSWLWDVDFEMLADARAEGKTD
                     AAPFTVSGIRARDMAVRLKYADLPVGPVIPDRREAIKTALEATPPGETLYVLPTYTAM
                     LEIRKTLSEMGYTHPFWED"
     gene            2140..2889
                     /locus_tag="AC16CM_04110"
     CDS             2140..2889
                     /locus_tag="AC16CM_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3139041.1"
                     /translation="MLTIHHLYADMMNLYGDRGNVISIKKRCEWRGISVDVIDVGLGE
                     KIEPTGCDLFLFGGGQDREQALLAEDLAGSKGADLRAIVEDGGVVLGVCGGYQLMGHS
                     YETPEGEKLPGVGIFDLHTEPRKVDEERLIGNVLVRIEAPETGGTREIVGFENHGGRT
                     YLGDGLGPLGEVIVGYGNNGKDGTEGARRLNAYGTYLHGSLLPKNPWLTDQLILNAIR
                     RVDEGFELEPLDDATEREAFSSVAGRVSGRR"
     gene            complement(2895..3608)
                     /locus_tag="AC16CM_04115"
     CDS             complement(2895..3608)
                     /locus_tag="AC16CM_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139042.1"
                     /translation="MSERVAAVLGVGPGLGAAVARRFAGEGFDVALMARREESVAGVR
                     EEIEGGGGTALSVSTDATDPDSVASAFELVRGELGDPEVFIYNAGAFQMGGILDLSPE
                     QFDACFRANCAGAFYAARQVLPAMVEAGRGTVLLTGATAALRGSARFSALAVGKFGLR
                     ALAQSMAREFGPQGIHVSHVIVDGQIDTPRVREMSPGREEHTFLSPDAIAETYWQLHS
                     QDPTAWTLELDLRPAVESF"
     gene            complement(3671..4189)
                     /locus_tag="AC16CM_04120"
     CDS             complement(3671..4189)
                     /locus_tag="AC16CM_04120"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139043.1"
                     /translation="MKIRDAVAADLPAIVEIYNSTIPGRMVSADTEPVSIEQRLGWFE
                     EHEPSRRPIWVVEEGGEVIGWLSLSEFFDRRPAYHATAEIGVYIREDRRGGGLGRRLV
                     EEAIRRGPELGLKTLTAGAFAHNAASIKLFERMGFREWAHFPRVAELDGVERDLVVLG
                     LRLDDREATRQD"
     gene            complement(4186..4863)
                     /locus_tag="AC16CM_04125"
     CDS             complement(4186..4863)
                     /locus_tag="AC16CM_04125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139044.1"
                     /translation="MHGRGRGRDGPLSGPHRRTVESGYDLIAEQYLATKSGDDPLSKE
                     ALEDLASLLPPAAAVLDLGCGAGVPATRWFAERGFAVTGVDVSARQLDLARTHVPEAT
                     FIKADMAEVGFEPGTFDAVVAFHSIIHVPRTEHPALLGRIHRWLKPNGAFLATMTITD
                     YEGRDEDWEGWGAPMVWSHFDADTNLAMLRDAGFGILYAEPRTGGGTGHEDETWLWVL
                     ARKDAAS"
     gene            complement(4827..5582)
                     /locus_tag="AC16CM_04130"
     CDS             complement(4827..5582)
                     /locus_tag="AC16CM_04130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139045.1"
                     /translation="MERLRGKRSIVTGAGSGIGRAIALRLAAEGANVLVSDVDETAAR
                     AVARELGGEALVRKTDVTSSGEVEALVAAAVDEWDGLDVMVNNAGVGVAGTVTNTSEK
                     DYEMVMDVCLRGTFLGMKHAIPAIRDSGGGSVINLSSVAALVGITDRAVYSAAKGAIL
                     AITRAAAIDHVEEGVRVNCIAPGTVDTPWIGRITSGYEDPQAARRNMQARQPHGRFVS
                     PEEIAAMAAYLASDESSSTIGACMVVDGGVTAR"
     gene            5669..5998
                     /locus_tag="AC16CM_04135"
     CDS             5669..5998
                     /locus_tag="AC16CM_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139046.1"
                     /translation="MNPYALWIQNLPQVDTPFDGMDGRMISGPDGQAVFFRAAERVEV
                     PAHSHGAQWGVVVSGLLHLTINGEEGTYRPGETYDIPDGAEHSAILEAETCVIDVFAD
                     PDRYSPK"
     gene            complement(6009..6782)
                     /locus_tag="AC16CM_04140"
     CDS             complement(6009..6782)
                     /locus_tag="AC16CM_04140"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3139047.1"
                     /translation="MKPNPLKRNLEEGGTALGTMVFEFATPGVARIAASAGAEFVVFD
                     QEHSGWGIETIRMLMATARAAELVPLVRVPSTQHHFISRPLDVGASGVMVPMVETAEQ
                     ASAVVTAAKYPPEGERGAAFGIGHDDYEAAEDVVSGMQQANDETLLIAQIETTDGIEN
                     VEEIAAVDGVDVLWIGHNDLTNSIGIPGQFDHPDYLRAVDRFLEACADNGKAPGIMST
                     SVEEARFQVDQGFRCVAYWGDIWLYASVLREGIERIREK"
     gene            complement(6788..>7622)
                     /locus_tag="AC16CM_04145"
     CDS             complement(6788..>7622)
                     /locus_tag="AC16CM_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139048.1"
                     /translation="TVEGADRLALVARFGVGYDDVDLGACTRNGVAVTITPDGVRRPM
                     ATSILALLLALSHRLLIKDRLARGGRWEERLDHMGTGLTGKTLGSVGLGNIGREMFTL
                     ARPLGMRHLAHDPYATPEMAAEVGAELTELEALLGTSDFVAINCALTPETHHLLDAGR
                     LDLMKSTAFVINTSRGQIVDQAALTGALRAGRLAGAGLDVFEEEPADPNDPIFSLPNV
                     VCAPHALAWTDELALGNGRSACESVLDVAAGRVPGNVVNREVLASPRWRDKLAMYAGR
                     T"
BASE COUNT         1307 a         2561 c         2474 g         1280 t
ORIGIN      
        1 tctggggcag cgcggcacgc acgaaacgca agagcggcca gaccagctcc tgaaggtagg
       61 cctcgaaatc cgagtcctcg aactcggtgg tctccgtcaa catgttggtc acgggcatca
      121 tgctcggcgg gcccgcgaag ttggcgacca gcacatcgat cctacccgcc tcctcgacca
      181 cgctcttgat ggtggccgga tcagtgtagt cggactggtc ggcgatgacg tcggccccct
      241 cctccctgaa gagctcgacg accggcgggc cgatgtagcg ctcagcgtgc gttacgagta
      301 cccgacggcc ttcaagacgg ccactcatcg acctcgcctc cgattacact tctgtggtct
      361 gcggaacata gcggccccac ccctccctgg ttgctcgcct taccggttct ccattcacta
      421 tacaagagcc ccgcagcacg gacccttaca ggtccttgat cctcaccgcg cgccctgcct
      481 ccccccgcgt gctcggggtg gtgcccactg tggatataat cgtcgccatg aaaccgttgg
      541 atccacgtct cttcgccgcg atactggccg ccgggtcggc gaggctcgcg agccgcacgt
      601 taaagaccgg tggggggtcc acgatgccag gggtcgtagc acggagggtg gacccgaagg
      661 tgctgaccaa gctctcgcgc cggctctctg gggggtcggc ggcgatcacg ggcaccaacg
      721 gcaagacgac caccaccagg atggtctcca cgatactgcg ttccgcgggt atcagggccg
      781 tcaacaactc gacgggcgcg aacctggtga ccggcgtaac cgcggcgctc gtggctgatt
      841 cgggcctatc gggcacgccg tcttcagaga tggggctctt cgaggtggac gaggcgagcg
      901 tccccagggt ggctgccgag gcggagatcc ggatcctcgc cgtcctgaac ctcttcaggg
      961 atcagctcga ccgctacggg gaacttgcct acacggggaa ggtgatctcc tcttctttcg
     1021 gcgacctgcc gcgggacggc gcggtggtac tgaacgccga cgacccactg gtggcgagcc
     1081 tggggcgctc cgccggaagg ccggtcttct acggcgtgga cgaaccagcc ctcgacaccg
     1141 ggcgtctgca gcacgtcgcc gactcgaaag actgcccctt gtgcggcacc gccctcgact
     1201 acgggaccgt ctacatgggg cacgtcggcg tctatgactg tccccgttgc gacttctcgc
     1261 gccccacacc cgactaccgg gcgagccggg tacggctcga aggggccggg ggctcgagct
     1321 tcctactctc gaccccgaag ggcgaggtgg aggcgaaagt gtcccttccc ggcctctaca
     1381 acgtctacaa cgcgctcgcg gcggcggcgg tggcgggcga agccggtgtc gggctccagg
     1441 agatatcgcg cgggctcggg gagttcggcg gcgctttcgg cagggtcgag cgggtaaggg
     1501 ccggggatag ggaggccttc ctgctgctca tcaagaaccc cgtcggcttc aacgagatcc
     1561 tacgcacctt cgtcacgggc gccgacgcca aaaacgtcct gatagccata aacgacaacg
     1621 acgccgacgg ccgcgacgtc tcctggctct gggacgtgga cttcgagatg ctcgccgacg
     1681 cccgcgccga aggaaaaaca gacgctgccc ccttcaccgt cagcggcatc agggcccggg
     1741 acatggccgt gcgcctcaag tacgccgacc tccccgtggg ccccgtaatc cccgaccgca
     1801 gagaggcaat aaagaccgcc ctcgaagcca ccccgccggg cgaaacgctc tacgtgctac
     1861 ccacctacac agctatgcta gagatacgca agacgctgag cgagatggga tatacgcatc
     1921 cgttctggga ggattagcag tcagcagtca gcagtcagca gtcagcagtt agctatcagc
     1981 ggtcagccag aaacgttggc cgacgtatcg ttggaacgag gcattaaccc gacgggtggg
     2041 cttgcagcaa cgaccaacat gttcgagact tcgatgcgaa acactgcgca aaaatggctg
     2101 aaagctgata gctaaaagcg ccgaccgaag ggaggcaaaa tgctaaccat ccaccacctc
     2161 tacgccgaca tgatgaacct gtacggggac aggggcaacg ttatctccat aaagaagcgt
     2221 tgcgagtggc gcggtatctc cgtggacgtc atagacgttg ggcttgggga gaagatagaa
     2281 ccgacgggct gcgacctgtt cctcttcggc ggtgggcaag acagggagca ggccttgctc
     2341 gccgaagatc tcgcgggctc caagggggcc gacctcaggg ccatcgtgga ggacggaggg
     2401 gtggtactcg gggtgtgcgg cgggtatcag cttatgggtc actcctacga gacgcccgag
     2461 ggcgagaagc tgcctggcgt gggcatcttc gatctccaca cggagccgag gaaagtcgac
     2521 gaggagcgcc tgatcgggaa cgtgctcgtc cgcatcgagg cccccgaaac cggcggcacg
     2581 cgcgagatcg tcggtttcga gaaccatggc ggacggacgt atctgggcga cggtctcggg
     2641 ccgcttggcg aggtcatcgt gggttatggc aacaacggca aagacggcac cgaaggggcg
     2701 aggcggctca acgcctacgg tacctacctg cacggctccc tgctacccaa gaacccctgg
     2761 ctcaccgatc aactgatact gaacgccata cgccgggtgg acgaaggctt cgaactcgag
     2821 cccctcgacg acgcgacgga gcgcgaggct ttctcctcgg tggccgggcg cgtgtccggc
     2881 aggcgctagg gtactcagaa gctctcgacc gctggccgca ggtccagctc cagggtccag
     2941 gccgtggggt cttgcgaatg gagttgccag taggtttcgg cgatggcgtc gggggagaga
     3001 aacgtgtgct cctcccgacc gggggacatc tcgcgaaccc tcggggtgtc gatctggccg
     3061 tccactatca cgtgcgagac gtggatgccc tgtggcccga actcccgcgc catggactgc
     3121 gcgagcgccc tcaagccgaa cttgccgacc gcgagcgccg agaagcgtgc cgagccgcgc
     3181 agggcggcgg tggcccccgt caggaggacc gtgcctcgtc ccgcctcgac catggccggg
     3241 agtacctgcc gggccgcgta gaaggccccc gcgcagttgg cccggaagca cgcgtcgaac
     3301 tgctccggtg agaggtccag tatcccgccc atctggaagg cacccgcgtt gtagatgaag
     3361 acctcggggt ccccaagctc gccgcgcacc agctcgaagg cggaggccac ggagtcgggg
     3421 tccgtggcgt cggtcgagac ggagagtgcc gtgcccccgc cgccctcgat ctcctccctg
     3481 accccggcga cgctctcttc ccggcgcgcc atcaacgcga cgtcgaatcc ttcaccggcg
     3541 aagcgccggg ccaccgccgc gccaagccct ggtccgaccc cgagaaccgc ggccaccctc
     3601 tcgctcatac cccgctcctt ctgtattgtg tgtacgtgag atcgccgggg aacacgttcc
     3661 ccgggtaacc ctaatcctgc ctcgtggcct cccgatcgtc cagccgcagg cccagtacca
     3721 ccaggtctct ctcgacgccg tcgagctcgg ccacgcgtgg aaaatgggcc cactctcgga
     3781 aacccatcct ctcgaagagc ttgatgcttg cagcgttgtg ggcgaaggcc cccgcggtca
     3841 gggtcttcag gcccagctcg gggccacgcc ttatggcctc ctcgaccagg cggcggccca
     3901 ggcccccgcc gcggcggtct tcccttatgt agactccgat ctcggcggtg gcgtggtagg
     3961 cgggcctcct gtcgaagaac tcgctcaggc tcaaccagcc gatcacctcc ccaccctctt
     4021 ccaccaccca gatgggacgc cgcgagggct cgtgctcctc gaaccatccc aacctctgct
     4081 cgatggagac gggctcggtg tcggccgaca ccatcctgcc cgggatggtg gagttgtata
     4141 tctccacgat ggcgggcagg tcagcggcga ctgcgtcgcg tatcttcacg aggccgcatc
     4201 cttgcgcgcc agcacccaga gccaggtctc gtcctcgtga cccgtgcccc caccggtgcg
     4261 gggctcggcg tagaggatcc cgaagcccgc atcgcgcagc atcgccaggt tggtgtccgc
     4321 gtcgaagtgg ctccacacca tgggcgctcc ccagccctcc cagtcttcat ccctgccctc
     4381 gtagtccgtg atggtcatgg tggcgaggaa agctccgttt ggtttcagcc agcggtggat
     4441 gcgcccgagc agggcggggt gttcggtgcg cggaacgtgg atgatggagt ggaaggcgac
     4501 caccgcgtcg aacgtccccg gttcgaagcc gacttcggcc atgtcggcct tgatgaaggt
     4561 ggcttctggg acgtgggtcc tggcgagatc cagttgcctc gcggagacat cgacgccggt
     4621 gacggcgaag cccctctcgg cgaaccagcg ggttgcggga acgccggcgc cgcagccgag
     4681 gtctagcacg gctgccgccg gcggcaggag ggaggcaagg tcttccaggg cctccttcga
     4741 gagcggatca tcgccgctct tggtggccag gtattgctcg gctatcaggt cgtatcccga
     4801 ctcgacggtt cggcggtggg gcccgctcaa cgggccgtca cgcccccgtc cacgaccatg
     4861 catgccccta ttgtcgagga ggattcgtcg gaggccaggt aggcggccat cgccgctatc
     4921 tcctccggcg agacgaatcg accgtggggc tggcgagcct gcatgttgcg gcgtgccgcc
     4981 tgcgggtctt cgtatcccga ggtgatgcgc ccgatccagg gcgtgtccac cgtgcctggc
     5041 gcgatgcagt tgaccctcac cccctcttcg acgtggtcga tggcggcggc ccgggtgata
     5101 gcgaggatgg cgcccttggc agcgctgtag acggccctgt ccgttatacc gaccagggcc
     5161 gccacggacg acaggttgat gaccgacccg cccccggaat ctcggatggc cgggatggcg
     5221 tgcttcatcc ccaggaacgt gcccctcagg catacgtcca tcaccatctc atagtccttc
     5281 tccgacgtgt tcgtgacggt tccggccacg cctaccccgg cgttgtttac catcacgtcg
     5341 aggccgtccc attcgtcgac ggcggccgcc accagcgctt cgacctcccc tgagctcgtt
     5401 acgtccgttt tcctgacgag ggcttcgccg cccagctccc tggcgaccgc gcgcgccgcc
     5461 gtctcgtcca cgtccgaaac cagcacgttg gcgccttcag cggcgagccg cagcgctatg
     5521 gcgcgcccga tacccgagcc cgcgccggtc actatggatc tcttgcccct caagcgttcc
     5581 atctcacccc cgaagtggtt ggagcgacca gtataatgac gccccgactg gcggtgcaaa
     5641 cgcaccttgc cgtaggaaag gagacgcttt gaacccttac gcactctgga tacagaacct
     5701 cccgcaggta gacacgccct tcgacggtat ggatgggcgg atgatctccg gccccgacgg
     5761 ccaggcggtg tttttccggg cagcggagag ggtggaggtt cctgcccact cccacggcgc
     5821 gcagtggggt gtggttgtga gcggccttct ccatctgacc ataaacggcg aagagggcac
     5881 ctacaggcca ggcgagacct acgacatacc ggacggcgcg gaacactcgg ccatcctcga
     5941 ggccgaaacg tgcgtcatag acgtcttcgc cgaccccgac cgctacagcc cgaagtagcg
     6001 ccgcggcgct acttctcgcg aatcctctct attccttccc gcaggacgct tgcgtagagc
     6061 cagatgtcgc cccagtaggc cacgcaacgg aaaccctgat ctacctggaa ccgggcctcc
     6121 tctacgctgg tggacatgat tcccggcgct ttgccgttgt cggcgcaggc ttcgagaaag
     6181 cggtcgacgg cgcgcaggta gtcgggatgg tcgaactggc cggggatacc aatagagttc
     6241 gtcaggtcgt tgtggcctat ccagagaacg tccactccat caacggcggc aatctcctcg
     6301 acgttctcga tgccgtcggt ggtctctatc tgggcgatca agagcgtctc gtcgttggcc
     6361 tgctgcatac ccgagaccac atcttcggcg gcctcgtagt cgtcgtgacc gatgccgaag
     6421 gccgcaccgc gctcgccctc cggcgggtat ttagccgcgg tgacgacggc gctggcctgc
     6481 tccgcggtct ctaccatggg caccatgacg ccggaagccc caacgtcgag cggacgcgag
     6541 ataaagtggt gctgggtcga gggcacccgc accagcggta cgagctcggc cgcccgggcc
     6601 gtggccatga gcatccggat cgtctcgatg ccccagccag agtgctcctg atcgaagaca
     6661 acgaactccg ccccggcaga ggcggcgatg cgcgccacgc ccggcgtggc aaactcgaag
     6721 accatggtcc caagcgccgt gcccccttct tccaggttgc gcttgagcgg gttgggcttc
     6781 atgggcttta cgtccttcct gcgtacatgg cgagcttatc tctccaacgc ggcgaggcca
     6841 gcacttcccg gttgaccacg ttgccgggga cgcgtccagc cgccacgtcg aggacgcttt
     6901 cgcaggcgct acggccgttc ccaagggcca gctcgtcggt ccaggccagg gcatgggggg
     6961 cacacaccac gttgggaagc gagaagatag ggtcgttcgg gtcggcgggc tcctcctcga
     7021 acacgtcgag accggcacca gcgaggcgac cggcgcgcag tgccccggtc aaagccgcct
     7081 ggtcgacgat ctggcctcta gaggtgttga tcacgaacgc cgtactcttc atgaggtcaa
     7141 gccgccccgc gtcgagcagg tggtgggtct cgggtgtgag ggcacagttg atggcgacga
     7201 agtccgaggt gccgagcagc gcctccagct ccgtgagctc ggctcccact tcggcggcca
     7261 tctccggcgt ggcgtagggg tcgtgggcca ggtggcgcat accgagcggc cttgcgaggg
     7321 tgaacatctc gcggccaatg ttgccaagcc cgaccgatcc gagggtcttg cccgtcaggc
     7381 cggtgcccat gtgatccaac ctctcctccc agcgcccgcc gcgcgcaagg cggtccttga
     7441 tcagcagcct gtggctcagg gccaggagca gggccaggat agaggtcgcc atgggacggc
     7501 gcacgccatc aggggtgatg gtgacggcca cgccgttgcg ggtgcaggct cccagatcga
     7561 cgtcgtcgta gccgacgccg aagcgcgcga cgagggccag gcgatcagcg ccctcgacgg
     7621 tg
//
LOCUS       DBNGVD010000146         5215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4765924, whole genome shotgun sequence.
ACCESSION   DBNGVD010000146 DBNGVD010000000
VERSION     DBNGVD010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4765924"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16CM_04150"
     CDS             <1..461
                     /locus_tag="AC16CM_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR3139049.1"
                     /translation="ALCLWWLYFDVVSSAAEHRLREAQGQARVRMAIEAYTYGHFPIV
                     AGIIVGAVGVEGVLAHAGDVKPLGFFYAAALYGGIALYLAGHLFFKQRIDATLGVPRL
                     VTVVVLLLALPVAAFLPPLVGLAGLVLLLVVLIGVETRRYAASGTEARTG"
     gene            complement(552..1802)
                     /locus_tag="AC16CM_04155"
     CDS             complement(552..1802)
                     /locus_tag="AC16CM_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3139050.1"
                     /translation="MRGIEGNRPLRIGLLGSGFIAGFHLQALLGVRDVEVAGVFSPTQ
                     ANREALASTANRMGLGPCEAYGSVEELLASPAVDAIWILGPNDTRLGHMRAIGKAVAD
                     GAALLGVACEKPLGRNLSEAREMLRLAEDAGLNHGYLENQVFSTAVQRGKDVIWRRGV
                     PAAGRPYLARASEEHSGPHAPWFWLGERQGGGVLSDMMCHSVEVARYLLTEPGRDRES
                     LKLVSANATIANLKWTRPKYAEQLKREMGSVVDYVNRPAEDFARGTLTLEDETGETVI
                     IEATTSWSYVGPGLRIQLELLGPEYAMEFSTLNTGLKVFLSRNVTGAEGEDLVEKQSA
                     EQGLMPVLEDEAATYGYTLEDRHMVEAFRKGEEPLETFHDGVAVVEMLMALYRSAETG
                     NTVRLADEDLETYVPPVARGEHKG"
     gene            complement(1815..2762)
                     /locus_tag="AC16CM_04160"
     CDS             complement(1815..2762)
                     /locus_tag="AC16CM_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139051.1"
                     /translation="MRRLRLNPIAIALLLALVLFVVGGLVQPGFASYGQAMNILRLAA
                     FLGIVAAGQTLVIISGGEGIDLSVGAVITLGAILVFRFGDGQNDQALLALVLALGVGL
                     AIGAMNGLGITLLGIPPLVMTLGMAGVVQGLILVVTGGRIEGEAAPALTGLVSGSLVF
                     GIPGVVLLWLLLGGVMWVLLRRTAYGRRLFAAGTNRVTARLSGVRVSWVLVLTYALSG
                     MLAALGGIVLLGYTEQVFLTLGDPYTLPSVAAVVVGGTLLAGGVGSYYGTMAGALVLT
                     LLTSLLTALELPESARLIVYGVTLLILLSLYGRQKALRQ"
     gene            complement(2759..3724)
                     /locus_tag="AC16CM_04165"
     CDS             complement(2759..3724)
                     /locus_tag="AC16CM_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139052.1"
                     /translation="MGPRLRRVALRQSYAFALVLLVVAVAINFYLQPNFFQPGILSGN
                     LLLFVPLMFVAVGQTAVVVGGGIDLSLGAIVSMANVIFVSLMGAEPSTSRIMLALGAG
                     FLAGILAGALNGFCVAVLRFQPIVTTFATSFIFAGLALYVLPEPGGAMPSGLVGFYYT
                     NPLGIPVVLWVVAALLLLWGLLRSTRYGTYLYATGGRDLSAFVSGVPVSSVRFLSYCI
                     AGLMASAGALALALSTGTGNPIGGTDLMLQSIVAVVLGGTRLSGGQGGVAGSLIGVVI
                     LSLIQSIVSFSGAPSSWQTLINGLIVVAALAGPGLISLLGRGRRN"
     gene            complement(3742..>5215)
                     /locus_tag="AC16CM_04170"
     CDS             complement(3742..>5215)
                     /locus_tag="AC16CM_04170"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3139053.1"
                     /translation="ALADGSLDVQSGEVVALMGANGSGKSTLGKIITGAIVPDGGRLL
                     LDGEETRFSSPQAARRAGIAAVYQELSLVPDMTVAENVWLANEPVALGRVRRRERKEQ
                     TTRLLALFENTLGSYVGPDSLVSDLSPSERQIVEILKALSREPRVMILDEATASLGAQ
                     QVERLFELIGDWKAGGMALIFISHRMEEIFQVADRATVLRSGNTVGEALLKETTQEHL
                     VGLMIERGVAERRVERTADGGKTVLLETEDLRSPVLDGVNLQLREGEILGLGGLQGQG
                     QSDLLLALFGAVPHSGTVRLAGEEVHFSRPRQAMREGLAFVPGDRGGEGLLPVRSILE
                     NLLLPSWSRYGALLDMRRARRDAEETSRGLSLKMGSLDHPVSSLSGGNAQKVVLGKWL
                     MRDPKVLLLDDPTKGVDVGAKGEFYNILSDLRQRGVAIVLHSSDDEELLGLCDRVIVM
                     RDGAVRRELSGGDLNKPALVSASVGAVADPVDDPSSGGPV"
BASE COUNT          928 a         1768 c         1690 g          829 t
ORIGIN      
        1 cggccctttg cctgtggtgg ctgtacttcg acgtggtctc cagtgccgcc gaacaccggc
       61 tccgggaagc ccagggccag gctcgcgtca ggatggcgat cgaagcctac acctacggac
      121 acttccctat cgtcgccggc ataatcgttg gcgcggtcgg cgtagagggt gtgctcgcgc
      181 acgccggaga cgtcaagccg ctcggcttct tctacgccgc ggccctgtac ggggggatcg
      241 cgctctacct ggccgggcac ctcttcttca agcagcgcat agacgccacg ctcggcgtgc
      301 ctcgactcgt cacggtcgtc gtcctgctcc tggcgctgcc cgtcgccgcc ttcttgccac
      361 cgctcgtcgg gctggctggc ctggtgctcc tgctcgtggt cctgatcggc gtcgagacga
      421 ggcgctacgc ggcgtccggt accgaagccc gcaccggctg atactgaaac agggtgatct
      481 ccgctcgcgt ttgtctgact cggacgggat cagcagcgcg cctctctgcc ggccttctgc
      541 agggttggga atcagccttt gtgctcaccc cgggcaacag gggggacgta ggtctcgaga
      601 tcctcgtcgg caaggcggac ggtatttccg gtctcggcgg agcggtacag cgccatgagc
      661 atctcgacga cggcgacccc gtcgtggaac gtctcgagcg gctcctcacc cttgcggaac
      721 gcctcgacca tgtgacggtc ttccagggta tagccgtagg ttgcggcttc gtcttcgagc
      781 acgggcatga ggccctgctc ggcgctttgc ttctcgacga gatcctcgcc ttcggccccg
      841 gtaacgttgc gcgagaggaa gaccttgagc ccggtgttga gcgtgctgaa ctccatggcg
      901 tactccggtc cgagaagctc gagttggatg cgtaggccgg gccccacgta tgaccaggag
      961 gtcgtggcct ctattatgac tgtctcgccg gtctcgtcct cgagggtcag ggttccacgc
     1021 gcgaagtcct cggccggacg attgacgtag tcgacgacgg agcccatctc gcgcttgagc
     1081 tgctcggcgt acttcggcct ggtccacttg aggttggcga tggtggcgtt ggcgctcacg
     1141 agcttcaggc tctcgcggtc tctacctggc tcggtgagga ggtagcgggc cacctcgacg
     1201 ctgtggcaca tcatgtccga gagtacgccg cccccttgcc tctcgccgag ccagaaccat
     1261 ggggcgtggg gaccgctgtg ctcctcggag gcgcgggcga ggtagggcct gccggcggcg
     1321 gggacgccgc ggcgccagat cacgtccttg ccccgctgga ccgccgtgga gaagacctgg
     1381 ttctcgaggt agccatggtt gaggccagca tcctccgcga gccgcagcat ctcgcgggcc
     1441 tcggagaggt tacgaccgag cggtttctcg caggcgaccc caagaagtgc ggccccgtcc
     1501 gccacggcct tgccgatagc gcgcatgtgg ccgaggcggg tatcgtttgg gccgaggatc
     1561 cagatagcgt caaccgcagg ggaggcgagg agttcctcca ccgacccgta ggcctcgcac
     1621 ggcccgagcc ccatcctgtt cgccgtgctc gccagggcct cgcggttggc ctgtgtaggg
     1681 ctgaacacgc cggccacctc gacgtcgcgg acgccgagga gcgcctggag atggaagccc
     1741 gcgataaagc cgctcccgag aagcccgatc cgcagcggac gattcccttc gatccctcgc
     1801 atcgttacca tcctttactg tctgagcgct ttctggcgac cgtagagcga gagcaagatc
     1861 agcagggtga cgccgtagac gatcaggcgc gcagactccg gaagctccag ggcggtaagc
     1921 aggctggtca acagcgtcag gaccagggcc ccggccatcg tcccgtagta gctacccacg
     1981 ccgccagcca ggagcgttcc cccgacgacc acggcggcca cggaaggcag cgtgtacggg
     2041 tctccgaggg tcaggaaaac ctgctccgta tagccgagca agacgatgcc accgagggcg
     2101 gcgagcatcc cgctcagggc gtaggtcaac accagaaccc atgacacccg cacccctgag
     2161 agcctcgcgg taacgcgatt ggtgccagct gcgaagagcc tgcggccgta ggcggtgcgc
     2221 cgcaggagga cccacatgac ccctccaagg agtagccaca gcagcacgac accggggatg
     2281 ccgaagacga gagaacccga gaccaggccg gtcagagccg gggcagcctc gccctcgatc
     2341 ctgccgccgg taacgaccag gatcaggccc tgcaccaccc ctgccatacc gagcgtcata
     2401 accagcggag ggatgccaag gagcgttatt ccgagcccgt tcatagcccc gatagcgagc
     2461 cccacgccga gcgcgaggac gagcgcgagc agcgcctggt cgttctgtcc gtccccgaac
     2521 ctgaagacga gtatggcgcc gagcgttata acggcaccga cggagagatc tatcccttcg
     2581 ccgccactga tgatgaccag cgtctggccc gccgcaacga tcccgaggaa cgccgcaagc
     2641 ctcaggatgt tcatcgcctg accgtaggac gcgaagcctg gctgtacgag cccgccgacc
     2701 acgaacagga ccagggccag aagcagggcg atggcgatgg ggttcagcct gagcctcctc
     2761 aattccgcct cccacgtccg aggagggaga tcaggccagg accggcgagg gccgcaacca
     2821 cgatcaggcc gtttatgagg gtctgccagg atgagggcgc gccggagaag gagactatgc
     2881 tctggatgag gctgaggatc accaccccga ttaaggaacc ggcgacccct ccttgcccgc
     2941 cgctcagacg cgtgcccccg agaacgacgg ccacgatgga ctgcagcatg agatccgtcc
     3001 ccccgatggg gttgccggtc ccggtgctca gggctagcgc aagggcgccg gcggaggcca
     3061 tgagacccgc tatgcagtag gagaggaagc gcacagatga aaccggcacg ccgctgacga
     3121 acgcagagag atctcgcccc cccgttgcgt acaggtacgt cccgtaccgc gtggatctca
     3181 gcaggcccca cagcagtagc agagcggcca ccacccagag caccactggt atccccaggg
     3241 gattggtgta gtagaaaccg accaacccag acggcatggc gccgcccggc tcggggagca
     3301 cgtagagcgc cagcccggca aagatgaagc tcgtcgcgaa ggtcgtgacg atgggctgga
     3361 aacggagcac ggcgacgcag aagccgttga gcgccccggc caggatgccg gccagaaatc
     3421 ctgccccgag cgcgagcatg atgcgggacg tagacggctc cgcgcccatg aggctcacga
     3481 agatcacatt cgccatagag acgatcgcgc cgagcgagag gtcgatgccg cccccgacca
     3541 cgacggccgt ctgccccacg gcgacgaaca tgagcggcac gaagagcagg aggtttccgc
     3601 taagtatccc aggctggaaa aagttgggct gcaggtagaa gttgatagcc acggccacga
     3661 ccagcagcac gagcgcgaag gcgtacgact gcctgagggc gacccgacgt agccgtggtc
     3721 ccaaagaagt ccgcgcggca ctcataccgg cccaccggaa gacgggtcgt cgacggggtc
     3781 cgcaaccgcg cccacgctcg ccgacacgag ggctggcttg ttcaggtcgc cgcccgatag
     3841 ctccctacga accgcgccat cacgcatcac gatcacacgg tcgcacaggc ccagaagctc
     3901 ctcatcgtcg gagctgtgga gcacgatcgc cacccccctc tggcggagat cggagagtat
     3961 attgtagaac tctcccttcg cgcccacgtc gacgcccttg gtcgggtcgt cgaggaggag
     4021 caccttcggg tccctcatca gccatttgcc aagcaccacc ttctgggcgt ttccgccgct
     4081 cagcgagctc accggatgat ccaacgaccc catcttgagg ctaaggcccc gcgacgtctc
     4141 ctcggcgtct ctgcgagccc tgcgcatgtc gagcagggcc ccgtaacgcg accaggaggg
     4201 cagcaggagg ttctccagta tggagcggac cggcaggagc ccctcaccgc cccggtcgcc
     4261 tgggacgaac gcaaggccct cacgcatcgc ctggcgcggg cgcgagaagt ggacctcctc
     4321 tcccgcgagc ctgacggttc ccgagtgcgg aacggcaccg aagagggcga gcaggaggtc
     4381 cgattgccct tgtccttgca gtccgccgag cccaaggatc tcgccttctc ttagctgcag
     4441 gttcaccccg tcgagcaccg gggagcggag atcctcggtc tcgagcagga ccgttttccc
     4501 gccgtcggcg gtcctctcga cgcggcgctc cgcgaccccg cgctcgatca ttagccccac
     4561 caggtgctcc tgggtagttt ccttcaggag tgcttcacct accgtgttcc cgctccggag
     4621 tacggtcgcc ctgtcggcga cctggaagat ctcctccata cggtgcgaga tgaaaatgag
     4681 ggccatgccc ccggccttcc aatcaccgat gagctcgaag agccgctcca cctgctgagc
     4741 ccccaggctg gcggtggcct catcgaggat cataacccgc ggctcgcggc tcagggcttt
     4801 gaggatctcc acgatctgcc gttcgctggg cgagaggtcg gacacgagcg agtccggccc
     4861 cacataggat ccgagggtgt tctcgaagag ggccaggagg cgcgtggtct gttccttgcg
     4921 ctctcttctc cggacccgcc ccagggccac aggctcgttg gcgagccaga cgttttcggc
     4981 gaccgtcata tcggggacca ggctaagctc ctggtagacg gcggcgatcc ccgcccggcg
     5041 ggccgcctgc ggggaggaga aacgtgtctc ctccccgtcg aggagcagac gtcctccgtc
     5101 ggggacgata gcccctgtaa tgatcttgcc gagcgtgctc ttgccgctgc cgttggctcc
     5161 catcagcgcc acgacctcgc cggactgcac gtccaggctg ccgtctgcaa gcgcc
//
LOCUS       DBNGVD010000147         3497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3855147, whole genome shotgun sequence.
ACCESSION   DBNGVD010000147 DBNGVD010000000
VERSION     DBNGVD010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3855147"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="AC16CM_04175"
     CDS             <1..117
                     /locus_tag="AC16CM_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HIR3139054.1"
                     /translation="FVLTEVALAVSIRDNLTLNVVMLIYPIEAIKEWQAAGH"
     gene            204..473
                     /locus_tag="AC16CM_04180"
     CDS             204..473
                     /locus_tag="AC16CM_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR3139055.1"
                     /translation="MCRNIKTLFNFAPPATEDEVRAASMQYVRKITGFNKPSRANEEA
                     FYAAVEDIAEISERLLASLETAAPPKDREEEAAKARARSALRFGG"
     gene            complement(730..1158)
                     /locus_tag="AC16CM_04185"
     CDS             complement(730..1158)
                     /locus_tag="AC16CM_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3139056.1"
                     /translation="MGTFNSSKTFPYSVEDLAPVARDVMDHFESQDFEVVETNIPTGG
                     VQVSIRKGGTFKAIIGMKTALNIKIEPVANGTTVDAGVGIFGQQAIPTAITLLVFWPV
                     IIAQVWNMAQESKLDEEALDVAEESLKTHASKTPDGATAE"
     gene            complement(1241..1513)
                     /locus_tag="AC16CM_04190"
     CDS             complement(1241..1513)
                     /locus_tag="AC16CM_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139057.1"
                     /translation="MDRPWFDQETDVLLIDEYIVEMDSFKRIVQDGKITDTELLEQTL
                     RVVTLLRKLEDALTPEAKALATETFGEMAVLNALQALRMEASSTLP"
     gene            1872..3173
                     /locus_tag="AC16CM_04195"
     CDS             1872..3173
                     /locus_tag="AC16CM_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139058.1"
                     /translation="MTLAPHAQKTRDPRAGAKGSGVLARLAWGVCGLVLVLIACAVAL
                     TVPNDYAHWRVDFLVPVASAALVGALVASRQPRNPVGWFILGHALCFSLGEFGRQYAI
                     YGVLTEPGSLPFARAMIWPTYWAWYPGLVLLVVLLPLYFPDGRLVSRGWRWASWLAII
                     FGASATALAMVRPGDSEARGIPNPLGIESLGDGNLVLPSVLDVLMSAGWLSLGSVAVT
                     SLIVRFRRSRAQERKQLEWFLYAVVLVLMVNAASRVVPAESLPLAARELLFVATFEGL
                     WVAIGIAILRYRLYGIDVLINRTLVYGTLTASLAALYFGGIVLLQMLFDLLSGQQSTL
                     AVVASTLMIAALFNPLRHRIQSFIDRRFYRRKYDARRTLEALSSKLREETNLDALSND
                     LVGMVRDTLEPAHVSLWLRPTRGPAEDGNDREFSGSYPTLP"
BASE COUNT          594 a         1052 c         1113 g          738 t
ORIGIN      
        1 ttcgtgctca cggaggtcgc gctggcggtc tctatcaggg acaacctgac cctgaacgtc
       61 gtgatgctga tctaccctat agaggcgatc aaagagtggc aggctgcggg acactagccg
      121 cggacgggtg tggggagacc cggacacggg ccgtatactt gtcgggtagt acggttggtc
      181 acctggtctg gaggtctggg ctcatgtgca ggaatatcaa gacgctcttc aacttcgcgc
      241 cgccggcaac cgaggacgag gtacgcgctg cctcgatgca gtacgtgagg aagataaccg
      301 gcttcaacaa accgtcgagg gcaaacgagg aggccttcta cgccgcggtc gaagacatcg
      361 cggagatctc ggagcggttg ctcgcctcgc tcgaaaccgc cgcaccgccg aaggacagag
      421 aggaagaggc ggccaaggcc agagcacgtt ctgccctccg gttcggcgga taatcgtacg
      481 gtggcccgtg acggggccag ttggccccgc cacgggcgaa cactcaacgg gagccggtcc
      541 caggtaaagg agggaggggg ctggcccgag gagcgtggtt tacccggcgc gtgggaaatg
      601 ctgtgatggg tacggccgcg ccgggttccc catcctcacc cgatcaatgc gtcccggcgg
      661 accgataaat acaccggacg caaaatcagg cgatagcgcc ccgggctggg acgtcccgtt
      721 ctggttacgt tactctgcgg ttgccccgtc cggggttttc gaggcgtgtg tcttgaggct
      781 ctcttcggcg acgtccaagg cctcctcgtc cagcttggac tcctgcgcca tgttccacac
      841 ctgcgcgatt atcacgggcc agaagacgag cagggtgata gcagtaggga tggcctgttg
      901 gccaaagatg cccacccccg catctaccgt ggtcccgttg gctaccggct cgatctttat
      961 gttcagggcg gtcttcatgc ctatgatcgc cttgaaggtg ccgcccttgc ggatgctcac
     1021 ctgcaccccg cccgtgggta tgttcgtctc gactacctcg aagtcctggc tctcgaagtg
     1081 gtccatcacg tcccgggcga cgggtgcgag atcctcaacg gaatagggga aggttttgga
     1141 agagttgaaa gtgcccatca gatgccctcc tttcgtgcac gcaagaggcc gtgctttgtg
     1201 agtttacttc tggcggtatc ttcttctgcg ggttgggttg tcaaggcagg gtcgaagaag
     1261 cctccatgcg gagcgcctgc agggcgttca gcaccgccat ctcgccgaat gtctctgtgg
     1321 ccagggcctt ggcctccggc gtgagcgcat cctctagctt gcgcaggagc gtgacaaccc
     1381 tcaaggtctg ctccagtagc tcggtgtcgg ttatcttgcc gtcctggacg atcctcttga
     1441 aggagtccat ctcgacgatg tactcgtcga tgagcaagac gtcggtctcc tggtcgaacc
     1501 atggcctatc cataagtcag gcctcctttc ccacctgtac ggcgaattgt ttgctccgaa
     1561 cgctttgcgg gtccctgttc acactccccg gtagctactc tactgtgttt cccgaccccg
     1621 gactacgagg ttgcgcgccg gacgttcccg gggctctggg ccagatttcg cacctcctgt
     1681 tcctcccgaa ctccccccgt cagagccaag gctctcagta taaacatcga tgcgcactgc
     1741 tcgaatcgct caaatgagct agattcggtg gtgttctcga tgagatggcc gaacatgagg
     1801 acagagggac cttcgggaaa tactctttct ggttttccgg atgctccggt ggttagtccg
     1861 agtagaatgt catgaccttg gcaccgcacg cgcagaagac acgagatcct cgggccggcg
     1921 caaagggctc cggcgtgctg gctcgtctcg cctggggtgt ctgcgggctt gttctggtcc
     1981 tgatcgcctg cgcggtcgcg ctaaccgtgc ccaacgacta cgcccactgg cgcgtggact
     2041 ttctggtccc ggtcgcgtct gccgcgctgg taggcgccct ggtcgcctcc aggcagccgc
     2101 gcaacccggt cggctggttc attctgggtc acgccctgtg cttctcgctc ggggagttcg
     2161 ggcgacagta cgccatctac ggggtgctaa ccgagcccgg ttcgctcccg ttcgccaggg
     2221 cgatgatctg gccgacctac tgggcatggt acccgggcct ggtgctgctg gtcgtcctgc
     2281 taccgctcta ctttcctgac ggtcgccttg tctccagagg ctggaggtgg gcctcgtggc
     2341 tagccatcat tttcggcgct agtgcgaccg cccttgccat ggttcggcct ggcgatagcg
     2401 aggccagggg catccccaac ccgctcggaa tcgaaagcct gggcgacggc aacttggtgt
     2461 tgccgtcggt gctcgacgtc ctcatgtccg ctgggtggct ctcgctgggc agcgtcgcgg
     2521 tcacgagcct gatagtacgt ttcagacgct cgcgggcgca agagcgcaag cagctagagt
     2581 ggttcctcta tgctgtggtc ctggtgctaa tggtaaacgc ggcgagccgg gtggtcccgg
     2641 ccgagtcgct ccccctggcc gcgcgggagc ttctgttcgt ggccaccttc gagggcttgt
     2701 gggtggccat aggtattgcg atcctccgct accgtctcta cggcatagat gtcctgatca
     2761 atcgcacgct ggtttacggt accttgacgg cctcgcttgc cgcgctctac ttcggtggca
     2821 tagtcctgtt gcagatgttg ttcgatctgc tcagcggtca gcaatccacc ctcgcggtcg
     2881 tggcctcgac gcttatgatc gcagccctgt tcaacccgtt gaggcatcgc atccagtcgt
     2941 tcatagaccg ccgcttctat cgtcgcaagt acgacgcgag acgaaccctg gaggccctct
     3001 cctcgaagct gagggaggag acgaacctgg acgccttgag caatgatctc gtagggatgg
     3061 tcagggacac gttggagccg gcgcacgtga gcttgtggct gcgcccgacc cgcggtccgg
     3121 ccgaggacgg gaacgacaga gagttttctg gctcgtatcc cacgctgccg tgatgccggt
     3181 ctacctgtcc aggcgaccga gggcacgtcg cgaggggatc cgaggcctct agtctgggcg
     3241 gtggtctgga cgggtggcgt acgtgctgct aataatgggc gccgcgaaga gtttcattat
     3301 cctgaagtgc gtttcataga ggaggcaggt gcgtggccga gaacaagaat gaaaatggtc
     3361 tacaccccag agtcgtggag atggcccgcg gggagaactt tgcggcgctc acgacgatat
     3421 tgccagacgg tcacccccag acccaggtga tgtgggtcga tgccgacgat gaaaacctct
     3481 tgatcaacac cgaggtg
//
LOCUS       DBNGVD010000148         4463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1306282, whole genome shotgun sequence.
ACCESSION   DBNGVD010000148 DBNGVD010000000
VERSION     DBNGVD010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1306282"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /gene="cydC"
                     /locus_tag="AC16CM_04200"
     CDS             <1..1497
                     /gene="cydC"
                     /locus_tag="AC16CM_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HIR3139059.1"
                     /translation="HDVTFRLLARVRTWFYERLEPLSPAVLSRYRSGDLLSRIAKDVE
                     ELENVYLRSFSPVAVALVISLLTASLLYLFDSTLAVVVLAFLAFTGVGVPVLVGYMSA
                     GLGREQLVLRAELSARIVDGIQGVEDILAFGREEDERRGVARLQGELDRTQRRMSLVA
                     GLQGSLTDVMMNLALVATLLIAIPEVASGATGGVYLAVLALVVLGSFEAIAPLGEAFQ
                     TLGRSIAAGERLFEISDTVPQVVDPVHPAPFPENVAVEFENVAYRYQGPEGFELEGIT
                     FSLRPGSRVAVVGASGSGKSTLVNLILRFWDPETGVVRLGGRDLREYAQRDLRARLAV
                     VPQDTHVFDGTLRDNLLLSRPGATEAEVRVAVEAAQLSGFVGSLTGGLDGHVGEHGLR
                     ISGGERQRLAVARALLRDAPLLVLDEPTANLDTITERDMLAAVRAAMEGRTTLMITHR
                     LVAMEEMDEILVLDRGRIIERGTHVELVRSGGLYRRMLDVQGGILGAA"
     gene            1603..2961
                     /locus_tag="AC16CM_04205"
     CDS             1603..2961
                     /locus_tag="AC16CM_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3139060.1"
                     /translation="MDDALLVSRMQFAFTVTYHYLFPMLTMGLALLIFVLKSIYMRNG
                     DERYNESARFWGKIFAVTFVMGVVTGIPMEFQFGTNWAAFSRFTGDVIAQTLAMEGAF
                     AFFLESAFVGLFLWGERRFGQRLHWFSSLMVFLGTWASGYFIIATNSWLQNPVGYRVL
                     DNGNIQLEDYWAVLLNPWMFAQYAHNMGGAVVCGAFAMAGLGAFYLLAQKHEEHGRIV
                     VKVGVVAGVLASVWMLFPSGHFSSEQVANEQPVALAAMEGQFKTEGPAGIVLIGQPDV
                     ENRRIDNPIVVPRALSYLVYQNWNAEVRGLDAFPEKNWPDNIPLLYYSYHVMVGLGTI
                     FIAILSLAAFLLWRGRLYGARWMLWILMLAVPFPFIANTAGWFTAELGRQPWLAYGLF
                     RTSEGVSPLVSSGSVLFTLIGFAGMYLIMGLLYVVLMVREVNHGPGQEDEMLLSPDTR
                     TV"
     gene            2971..4011
                     /gene="cydB"
                     /locus_tag="AC16CM_04210"
     CDS             2971..4011
                     /gene="cydB"
                     /locus_tag="AC16CM_04210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR3139061.1"
                     /translation="METLWFVLVALMVAMYVLLDGFDLGAGALHPFVARNDRERRMVL
                     RAIGPVWDGNEVWLIAAGGTLFFAFPVLYASGFSGFYLPLIIVLWLLMIRGLSIELRG
                     HVDNPMWGSLWDASFFVGSALLTVFYGAALGNVVRGVPLNEDGYFFQPLWTDFSPFSS
                     TPGILDWYTVLIGLLAFTTLVAHGANYLAVKTEGGLNARARRYSRAAWLATAALTVLA
                     TPATFWVNPDLLEGLNQRPYGYVLPLIAIAGLAGMGYFNLTLRDRGAFLSSSAYIVGM
                     LTSTVFAVYPNVLPAVNAANSLTIYNASSSAYSQGVGLVWWSIGMVLAAVYFIVIYRL
                     FRGKVRLEDEGY"
     gene            complement(4157..>4463)
                     /locus_tag="AC16CM_04215"
     CDS             complement(4157..>4463)
                     /locus_tag="AC16CM_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139062.1"
                     /translation="PEDNEMRLPIFETVLGGIHVTGSIVGTRVDLQEVFELHAMGRTR
                     VVHEERKLEDVNEAIAEVESGEVDARLVFDLGTERPARRSEGVFTEERRSASEQPTG"
BASE COUNT          684 a         1454 c         1418 g          907 t
ORIGIN      
        1 cacgacgtga cgtttcgcct cctggcccgc gttcgaacct ggttctacga acgtctcgaa
       61 cccctctcgc ccgccgtgct ctcccggtac cgcagcggcg accttctctc ccgcatcgcg
      121 aaggacgtcg aggaactcga aaacgtatac cttcgttcgt tctcgccggt ggcggtcgcg
      181 ctggtcatct cactgctcac cgcctcgttg ctctacctgt tcgattcgac gctggccgtc
      241 gtggttctcg cgtttctggc gttcacgggc gtcggggtcc ccgtgctagt gggctatatg
      301 tctgccggtc tcggtcgcga gcagctggtg ctgcgtgcgg aattgagcgc ccggatcgtg
      361 gacggtatcc agggcgtgga agacatcctt gctttcggga gggaagaaga cgaacggcgc
      421 ggggtggccc gactccaggg ggaactcgac cgtacgcagc gtcggatgtc gctggtcgcc
      481 gggctccagg gatctctgac cgacgtcatg atgaacctcg ctctcgtagc cacgcttctg
      541 atcgccattc cagaggtcgc ctctggcgct accgggggcg tatacctcgc cgtcctggcg
      601 ctcgtggtac tgggcagctt cgaggccata gcaccgctcg gtgaggcatt ccagacgttg
      661 gggcgttcca tcgcggccgg agagcggctc ttcgagattt cggacacggt accgcaagtc
      721 gtggacccgg tgcatcccgc gccgttcccc gaaaacgtcg ccgtcgagtt cgaaaacgtg
      781 gcctaccgct accaggggcc agaggggttc gaactcgaag gtatcacttt ttcgctcagg
      841 ccggggagca gggtggctgt cgtcggggcg agcgggtccg gcaagagcac gctggtcaac
      901 ctgatcctgc gcttctggga tcctgaaacg ggcgtggtcc ggctcggcgg ccgcgacctt
      961 cgcgagtatg cccagaggga tctgcgcgca cggctggccg tcgtaccgca ggacacccac
     1021 gtcttcgacg gcaccctgcg cgacaatttg ctgctctcca ggcctggtgc cacagaagcc
     1081 gaggtacggg tggcggtcga ggcggcgcag ctctccgggt tcgtcgggag cctgacgggc
     1141 gggctcgacg gccacgttgg cgagcacggg ctccgcatca gcgggggtga gcgtcagcgg
     1201 ctcgcggtgg ctcgggccct gctgagggac gcaccgctgc tcgtcctcga cgagccgacg
     1261 gccaacctcg acaccatcac ggaacgcgac atgctggcgg cggttcgcgc cgccatggag
     1321 ggacgaacca cgctcatgat tacccaccgt ctagtcgcga tggaggagat ggacgagatc
     1381 ctggtcctgg accggggacg gatcatcgag cgcggcaccc atgtcgagct ggtccggtcc
     1441 ggcggcctct accgccgtat gctcgacgtg cagggcggaa tcttaggggc ggcgtgacga
     1501 tgggccggcc cacgttggta gggaattggt tacttttgat caggtacgcc gatcccgtgg
     1561 gctgtagcct gaaccagtcc ggccagcaca gaggagcctg ccttggatga cgccctgctc
     1621 gtatctcgta tgcagttcgc cttcacggtg acctaccact acctgtttcc gatgctcacg
     1681 atggggctcg ccctactcat cttcgtgctc aagagcatct acatgagaaa cggcgacgaa
     1741 cggtacaacg aatccgcgag gttctggggc aagatcttcg ccgtcacctt cgttatgggg
     1801 gtggtgacgg ggatcccgat ggagttccag ttcgggacca actgggcggc cttctcccgc
     1861 ttcaccggtg acgttatcgc ccagaccctg gcgatggagg gcgcgttcgc cttctttctc
     1921 gagtccgcct tcgtgggcct cttcctgtgg ggcgagcggc gcttcgggca acgcctgcac
     1981 tggttctcgt cccttatggt atttctgggg acctgggctt cggggtactt catcatagcc
     2041 accaactcgt ggcttcagaa cccggtcggc taccgcgtac tcgacaacgg aaacatacag
     2101 ctggaagact actgggccgt actactgaac ccatggatgt tcgcccagta cgcccacaac
     2161 atgggcggcg cggtggtgtg cggcgccttc gccatggccg gcctcggggc cttctacttg
     2221 cttgcccaga agcacgagga gcacgggcgg atcgtcgtga aggtcggcgt cgtggccggg
     2281 gtgcttgcga gcgtgtggat gctgttcccg agcggccact tctcgagcga gcaggtcgcg
     2341 aacgagcagc cggtcgctct cgccgccatg gagggccagt tcaagaccga aggccctgcc
     2401 ggcatcgtcc tcatcggcca gcccgacgtt gagaaccgtc gcatagacaa ccccatcgtc
     2461 gtgccgcgcg ccctgagtta cctcgtctac cagaactgga atgccgaggt gcgggggctc
     2521 gacgccttcc ctgagaagaa ctggccggat aacataccgc tcctctacta ctcctaccac
     2581 gtgatggtcg gccttgggac catcttcata gccatcttga gcctggcagc gttcctcctc
     2641 tggcggggtc ggctctacgg ggcgcgctgg atgctctgga tcctgatgct cgccgtccct
     2701 ttcccgttca tagccaacac agccggatgg ttcaccgccg agcttggccg gcagccgtgg
     2761 ctggcctacg ggctcttccg cacctctgag ggggtctcgc cgctcgtatc ttccggcagc
     2821 gtgctcttca cgctcatcgg gttcgccggg atgtacctga tcatggggct cctgtacgtc
     2881 gtgcttatgg tcagggaggt caaccacgga cccggacaag aggatgagat gctgctttcg
     2941 cccgataccc ggaccgtgta aggaggaact gtggagacgc tctggttcgt cctggtggcg
     3001 ttgatggtcg cgatgtacgt cctgctcgac gggttcgacc tcggcgccgg cgcgctacac
     3061 ccgttcgtcg cgagaaacga ccgcgagcgg cgaatggtcc tcagggccat cggccccgtc
     3121 tgggacggca acgaggtctg gttgattgcg gccggtggta cccttttctt cgccttcccc
     3181 gtcctctacg cgtccggctt ctccggcttc taccttccgc tcatcatcgt actctggctg
     3241 ctcatgatcc gcgggttgtc catagagttg cgcggtcacg tcgacaaccc gatgtggggc
     3301 tccctctggg atgcgtcgtt cttcgttgga agcgccctgc taaccgtctt ctacggcgcg
     3361 gccctcggca acgtcgtgcg gggggtaccc ctgaacgagg acggctactt tttccagcca
     3421 ctgtggacag acttcagccc gttcagcagc acgccgggca tcctcgactg gtacacggtc
     3481 ctgatcgggc tcctcgcctt cacgacgctg gttgcgcacg gcgcgaacta tctggccgtg
     3541 aagacggagg gcggcctgaa cgcccgtgcc cgccgctatt cacgtgcagc ctggcttgcg
     3601 accgccgcgc tgacggtact ggcgacccct gccacgttct gggtgaaccc ggatctgctc
     3661 gagggcctga accagcgccc ttacgggtac gtgctaccgc taatcgccat cgccgggctt
     3721 gccgggatgg gctacttcaa cttgacgctg cgcgatcggg gcgcgttcct ctcttcgagc
     3781 gcgtatatcg tcgggatgct gacgagcacg gtcttcgcgg tgtacccgaa cgtgttgccc
     3841 gccgtaaacg cggcgaacag cctcaccatc tacaacgcat cctcgtcagc ctatagccag
     3901 ggggttggcc tggtatggtg gagcatcgga atggtcctcg ccgccgtgta cttcatcgtg
     3961 atctaccgtc tcttccgtgg caaggtccgg ctcgaggacg aagggtactg atccgcctcg
     4021 agccgtccgg tacccggtca gaggtgggca cgtcttgtac cttctctcgc ccgaacagga
     4081 ggacgcctcg gatggacccg aggcgtcctt cgtgctggtg ctggcgaccc gcgatcgggc
     4141 ccttccccac cccggtctag ccggtgggct gctcggaggc gctacgccgc tcttccgtga
     4201 agacgccttc gctacggcgt gctggtcgct cggtgccaag gtcgaagact agcctggcat
     4261 ccacctcgcc cgactcgacc tcagcgatgg cctcattgac gtcctccagc ttcctctcct
     4321 cgtggacgac cctcgtcctg cccattgcgt gaagctcgaa gacctcctgc aggtccaccc
     4381 tggttcccac gatagagccc gtaacgtgga tgccgcccag aaccgtctcg aagatcggca
     4441 accgcatctc gttatcctcg gga
//
LOCUS       DBNGVD010000149        10636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2034800, whole genome shotgun sequence.
ACCESSION   DBNGVD010000149 DBNGVD010000000
VERSION     DBNGVD010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2034800"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..609)
                     /gene="msrB"
                     /locus_tag="AC16CM_04220"
     CDS             complement(193..609)
                     /gene="msrB"
                     /locus_tag="AC16CM_04220"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3139063.1"
                     /translation="MTDTPRKIQKTEEEWQDQLTSEQYRVTRMKGTERPFSGEYNHNK
                     EEGIYRCVACDAPLFSSDTKYDSGSGWPSFWAPLDENNVETEEDRSLFMRRIEVHCAV
                     CGAHQGHVFEDGPAPTGQRYCINSAALRFDPEAASE"
     gene            complement(788..3667)
                     /gene="treY"
                     /locus_tag="AC16CM_04225"
     CDS             complement(788..3667)
                     /gene="treY"
                     /locus_tag="AC16CM_04225"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR3139064.1"
                     /translation="MVPDGVRVPRSTYRLQLNGGFTFSDAAALVPYLAELGVGDVYTS
                     PYLKARPGSTHGYDVVDPTSLNPELGTEDDYRRLVRVLRDHGMGHLLDIVPNHMGVGP
                     DNAFWYDVLENGPASHYATFFDIDWSPAGKRELQGKVLLPVLGDHYRATLGRGELGLV
                     FDAEKGAFAVTYYEHRLPIDPRTYPMILEGLDPSEAGEQRGEIRDLVTAFGDLPGRDA
                     TDEQSVSIRAHDAASLKGGLADLCARSSEVSSAVEERVRRLNGEGFRALHPLLAAQAY
                     RLAHWRVAGDEINYRRFFAVNDLAGVRVEDEDVFAATHGLVLRLIGEGAVDGLRIDHP
                     DGLRDPTAYLGRLRDAIIRASGGPVYTLVEKILARGEDLPDDWPVSGTTGYDFMNLVN
                     GLFVDPEGEAGISEAYRTFLGEDIDFPGLLLRCKRKVMRGALASELKALSYRLLAISE
                     HGRSYDFGFETLRRALAEVVAHFPVYRTYIGPGRISGVDREHLDVAVREAKAGSDGDP
                     GAFDFIRDVVLWAPRDDAALDFVYRFQQYTGPVMAKGMEDQALYVYDRFVSLNEVGGE
                     PEHFGVSVTEFHRQNAKRLARWPHAMLSTSTHDTKRSEDIRARINVLSEIPEEWQERI
                     GNWRELNAPFRREIEGEPAPSPNDEYLLYQTLLGAWSLGELDEAMLEELRGRTKAYME
                     KAMREAQVSTSWTDPNDGYEKAVADFVDGILRLDGPFLVAFLPFQRSVARTGALNGLG
                     QTLLKMTSPGVPDVYQGNEIWDFSLVDPDNRRPVDFGRRKRLLTALRTLDASSVSTLL
                     DDGIWQDGGPKIHLIQRALETRRKSPELFADGEYLALETSGTYKENLVAFARMRGGEV
                     AITVAPRLCAKMPPAEGSLLPDRQAWRGTSILLPEELAGVSLRNVFTGETTTLEHDGR
                     PVLNAGRLLRTFPVALLEGVSRENMHGGAEAKLSPDIHGRLQDRI"
     gene            complement(3649..5394)
                     /gene="treZ"
                     /locus_tag="AC16CM_04230"
     CDS             complement(3649..5394)
                     /gene="treZ"
                     /locus_tag="AC16CM_04230"
                     /EC_number="3.2.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033942 -
                     4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HIR3139065.1"
                     /translation="MRRRHEMPLGAEVLEDGETRFNLWAPAAEDVDLVLEGDGIPMSR
                     QEDGTFSLTTRAGAGTRYRFRIDGGEEVPDPASRSQPDGVHGASAVVDPVSYEWRDWY
                     PGGRPWEEVVLYELHVGTFTPVGTFAGVEGRLDHLVGLGVTAIELMPLSEFPGRRNWG
                     YDGVLPFAPESAYGTPDELKNLVQSAHDRGLMVFLDVVYNHFGPEGNYLSRYAPQFFT
                     DRYDTPWGAAVNFDGEGSGHVREYFIHNALYWLEEYGFDGLRLDAVHAIKDSSETHFL
                     DELAGRVREGPGHHRHVHLVLENDAKEARYLRGPYDAQWNDDLHHALHVVMTGESAGY
                     YADYAGEPVADLGRALSGGFVHAEAPDTSLPPGVFVSFVQNHDQVGNRAFGERIGKLA
                     SPEAVRAAAEIYLLAPQTPMLFMGEEWNADSPFPFFCDFGQDLAPLVAEGRRAEFAGF
                     FEAEDADSIPDPGAEETFVRGKLDWASLDEQEHARHLELYRGLLALRRAEILPRLGGS
                     PGGESGYEAVGERGLRVWWRLGDDSCLTLLANLGPEPLDGVERPPGKILYAGDGVAKD
                     GPELPGWSALWYLTG"
     gene            complement(5391..7538)
                     /gene="glgX"
                     /locus_tag="AC16CM_04235"
     CDS             complement(5391..7538)
                     /gene="glgX"
                     /locus_tag="AC16CM_04235"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3139066.1"
                     /translation="MTSVVWPGHPYPLGATWDGQGVNFALFSENAEGVELCLFDASGK
                     EEVERIELREWTDRIWHCYLPYCRLGQLYGYRVHGPYDPENGHRFNPAKLLLDPYAKA
                     TVGSVDWEYPHFGYRLGAEEEDLALDRQDNAAGMPKCRVVDTAFTWDDDRPPRTPWED
                     TVIYELHVKGFTQRHPEVPEDLRGTYAGLASAPAIEHLKKLGVTAVELMPVHAFVDDR
                     HLAERGLKNYWGYNSIGFFAPDMRYAASGSISEFKTMVKRLHSAGLEVILDVVYNHTA
                     EGNRLGPTLSFRGIDNAAYYRLVPDDLRHYMDFTGTGNTLNMMHPRVLQLIMDSLRYW
                     ILEMHVDGFRFDLASALARQLYEVDALSSFFTIIHQDPVISQAKLIAEPWDVGEGGYQ
                     VGNFPVGWAEWNGKYRDEVRSYWKGDGGIVGELAYRLTGSSDLYERGGRRPYASINFV
                     TAHDGFTLSDLVSYDRKHNEANGENNNDGESHNLSRNYGVEGQTEDLEIRRTRARQRR
                     NIMATLLLSQGVPMILSGDEMRRTQGGNNNAYCQDNETSWLDWNLSAPDKNFLAFVRR
                     MIRLRTEHPAFRRKSFFQGRELRGADAKDITWLTPEGREMTDEEWNNSFARSLGLHMS
                     GLLEGEHDAQGRPQVDDDFLLLFNADENGADFRLPTSPEDVRWEVFMDTSYAAGLRAA
                     RFLKPDDVYKLKPRSMAVLVNYRRRETVDEDAD"
     gene            complement(7638..9809)
                     /gene="glgB"
                     /locus_tag="AC16CM_04240"
     CDS             complement(7638..9809)
                     /gene="glgB"
                     /locus_tag="AC16CM_04240"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3139067.1"
                     /translation="MRQVEAVAAIVAGDHGDPFSFLGMHASEDGPVVRAFLPGARSVE
                     VVSREGDAVGVLERIHPDGFFAGAVRQSVPYRLRVRWEEGGEDEVEDPYRFPGVLGDQ
                     DLFLFGEGTHLGLYEKLGAHPTELEGVAGTSFAVWAPNAKRVSVVGNFNFWDGRKHPM
                     RKHPGGVWEIFLPGVGDGEVYKYEILGPNGELLPLKADPFGFRAERLPGTASVVHDPT
                     RFEWNDEAWMRGREGANALDAPMAIYEVHLGSWRRNPDGSYYTYGQLAEELVPYVAEM
                     GYTHVEFLPPTEHPFDGSWGYQPLGLFAPTSRFGTPDDFKDLVDAFHREGIGVFIDWV
                     PAHFPEDQHGLALFDGTHLYDHADPRQGRHPDWGTLVFNYGRPEVRNYLLSNALFWLS
                     EYHVDGLRVDAVASMLYLDYSRNPGEWIPNVHGGNENLEAVSFLRRANEVVFGGHPGA
                     TTLAEESTAWPMVSRPTSMGGLGFGYKWNMGWMHDSLQYMSEDPINRRYHHNEITFSL
                     AYAFGENFVLPLSHDEVVHGKGSLLEKMPGDEWQQFANLRAYYGFMYAHPGKQLLFMG
                     GEIAQRREWNHDISLDWHLLQEPSHEGIQALTKDLNALYRTTPALYRVDFDPAGFEWI
                     SGGDIENSVVSFLRRTDAEEDLAIVVCNFTPVVRYDYRVGVPEAAAYEETLNTDDARY
                     GGSGVGNTGRISTQRVEAHGREHSIVLTLPPLATVVLKPLG"
     gene            complement(9806..>10636)
                     /locus_tag="AC16CM_04245"
     CDS             complement(9806..>10636)
                     /locus_tag="AC16CM_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR3139068.1"
                     /translation="RLLTSWSPEGEDAPDEELAPEDTFFASLVRILGLRTGELHAAFA
                     APTDDEAFAPEMASPEEVAGWVDGVLAEAGSTLDLLKRRGSSLPDEVAPDVERLLGLR
                     EELRRSVKSVAGQNFGVVKTRHHGDYHLGQVLVVSNDFEIIDFEGEPARPLAERRKKH
                     SPLRDVAGMLRSFDYAIRSALMDLGADRADQLETLEPWVRLWKERTGRSFLDGYREGV
                     GDAASYPEDEEQARALIGLFTLEKALYEIRYELDNRPDWVGIPIKSVLDLLEDGEEDL
                     "
BASE COUNT         1768 a         3459 c         3464 g         1945 t
ORIGIN      
        1 gtagccaagt gctatctacc cccagaattt ctcgcctgaa gcaagattag aggaaagaca
       61 tcaggaaaag tggatcctgg aacacaaaac ctcgccggca cagcgaaatg tcatggtatc
      121 tacgctcttc agagcccggg cggctatcag ctttgatcaa agctgatagc cgccgctgaa
      181 agctggaagc ttttactccg aagcggcttc gggatcgaag cggagggcgg ccgagtttat
      241 gcaatagcgc tgcccggtcg gggccggtcc gtcctcgaat acgtgcccct gatgggcccc
      301 gcacaccgcg cagtgaacct ctatcctgcg catgaagagg ctacggtcct cctccgtctc
      361 cacgttgttc tcgtcaagag gcgcccagaa gcttggccat ccgctgcccg aatcgtactt
      421 ggtatcggac gagaagagcg gcgcgtcgca ggccacgcaa cggtagatcc cctcttcctt
      481 gttgtggttg tactcgccgc taaagggccg ctctgtcccc ttcattcgcg tgacacggta
      541 ctgctcagaa gtgagctgat cctgccactc ctcttccgtc ttctgtatct tccttggcgt
      601 atcagtcatg cgcaaattct agcacgccgc ggtttcgcct ccgcttgttg gcatgcccgg
      661 gctaacaccc tcgcttgcca ggctttcagc tcgggggcta gaggccgtta tggactcgat
      721 gaggaaaggc ccaggcagcc ggaaaccgga agaccgcgca agaccgagtc aaccggagaa
      781 ttcgcgttca aatgcggtcc tgaagtcttc catggatatc tgggctcaac ttggcttccg
      841 ctccaccgtg catattttct cgagaaacac cctctagcaa ggctacgggg aaggtgcgga
      901 gaagtcgccc ggcgttgagg acgggccttc cgtcatgctc gagggttgtt gtttcgccgg
      961 tgaacacgtt acgaagcgag acaccggcga gctcctctgg aaggagaatg gaggtgcccc
     1021 gccaggcctg ccggtccgga aggagggatc cttctgcggg gggcatcttc gcgcagagcc
     1081 gtggagcgac ggtgatggcg acctctcccc cgcgcatccg ggcgaaggcg acgaggttct
     1141 ctttgtacgt gcccgaggtt tcgagcgcca agtactcgcc gtccgcgaag agttccgggc
     1201 tcttgcggcg cgtttcgagc gctctctgga tcaggtgaat tttcgggcca ccgtcctgcc
     1261 agatgccgtc gtcgagcagc gtggatacgc tggaagcatc cagcgttctc agggcggtga
     1321 gcagcctctt gcgccgcccg aagtccaccg gacggcggtt gtcggggtca acgaggctga
     1381 agtcccagat ctcgttcccc tggtacacgt cggggacgcc gggcgaggtc atcttcagga
     1441 gggtctggcc caggccgttc aaggcgccgg tgcgggccac acttcgctgg aacggcaaga
     1501 acgctaccag gaaagggccg tccaggcgga gaatcccgtc cacgaagtct gcgaccgcct
     1561 tctcgtaccc atcgttcggg tcggtccagg aggtgctgac ctgagcctcg cgcatggcct
     1621 tctccatata ggccttcgtc cgcccgcgga gctcttctag catcgcttcg tccagttcgc
     1681 ccaacgacca ggcgccgagg agtgtctggt agaggaggta ctcgtcgttg gggctagggg
     1741 ctggctcgcc ttcgatttcg cggcggaacg gggcgttcag ttccctccag tttccgatcc
     1801 tctcttgcca ctcctcggga atctcggaga gaacgttgat gcgggctcgg atatcctcgc
     1861 ttcgcttggt gtcgtgcgtg gaggtcgaga gcatcgcgtg cggccagcgg gcgagacgct
     1921 tggcgttctg gcggtgaaac tcggtgacgg agacgccgaa gtgttccggc tccccgccga
     1981 cctcgttcag ggagacgaag cggtcgtaca cgtagagggc ctgatcctcc attcccttcg
     2041 ccataacggg ccccgtgtac tgctggaacc ggtacacgaa gtcgagggcc gcgtcgtccc
     2101 ggggagccca cagcaccacg tcgcggataa aatcgaaggc gcccggatcg ccgtcgctcc
     2161 ccgctttcgc ttcccgcacc gcaacgtcga ggtgctcccg gtcgactccg gagatacgcc
     2221 cagggccgat gtaggtccgg tacaccggga agtgggccac aacctccgct agcgccctgc
     2281 ggagggtctc gaagccaaaa tcgtacgagc gtccgtgctc ggagatcgcc agcaggcgat
     2341 aggagagcgc cttcaactcg ctagccagcg ccccgcgcat caccttgcgc ttgcaacgca
     2401 gaagtagccc cggaaaatcg atgtcttccc cgaggaaggt cctgtaagcc tcgctgatgc
     2461 cggcctcccc ctccgggtcc acgaacaacc cgttcaccag gttcatgaaa tcgtagcccg
     2521 tcgttcccga gaccggccag tcgtccggca gatcctcccc acgggccagt atcttctcga
     2581 ccagcgtgta aaccggccct ccagacgccc tgataatggc gtcgcgcaga cggccgaggt
     2641 aggcggtagg atccctcagc ccgtcggggt ggtcgatgcg cagcccgtcg acggcaccct
     2701 ccccgatcag gcgcagcacg agcccgtgcg tggctgcgaa gacgtcctcg tcctcgaccc
     2761 ggacacccgc gaggtcgttg acggcgaaga agcgccggta gttgatctca tcgccggcta
     2821 cccgccagtg ggcaagcctg taggcctgcg ccgcgagcag cgggtgcagg gcccggaagc
     2881 cttcgccgtt gagccgccga acgcgctcct cgacggcgct cgagacctcg gatgaccgcg
     2941 cgcacaggtc ggcgaggcca cccttcaggc ttgccgcgtc atgcgcccgg atggagacgc
     3001 tttgctcgtc cgtcgcatcc cggccgggta ggtcgccgaa ggctgtcacg agatctctga
     3061 tctctcctcg ctgctcaccc gcctcagaag ggtccaggcc ctcgaggatc atagggtagg
     3121 tgcgggggtc gatcgggagg cggtgctcgt agtaggtcac ggcgaaggca cccttctcgg
     3181 catcgaagac cagcccgagt tctccgcgtc cgagggtggc gcggtagtga tcgcccagta
     3241 ccggcagcag gaccttcccc tgtagctccc gcttgcccgc gggagaccag tcgatgtcga
     3301 agaaggtagc gtaatgcgag gcggggccgt tctccagcac gtcgtaccag aaggcgttgt
     3361 cggggccgac gcccatgtgg tttggaacga tgtcgaggag gtggcccata ccgtgatccc
     3421 ggagaacccg aaccagacgc cggtaatcgt cctcggtccc gagttccggg ttgagcgagg
     3481 tagggtcgac cacgtcgtag ccgtgcgtgc tgcccggcct ggctttcagg tagggcgagg
     3541 tgtagacgtc gccaaccccg agctcggcca ggtagggtac gagtgcggcc gcatccgaga
     3601 acgtgaagcc cccgttgagc tgcagcctgt aggtggagcg gggtaccctc accccgtcag
     3661 gtaccacagc gcagaccagc ccggaagctc cggcccgtct ttcgccacgc cgtcgcccgc
     3721 gtagagaatc ttgcccgggg gccgctctac cccgtcgagc ggttcagggc cgaggttggc
     3781 caggagcgtg aggcaggagt cgtcgccgag cctccaccac acgcgcagtc cccgttcgcc
     3841 caccgcctcg taccccgatt cgccaccggg ggagccgccg aggcggggca ggatctccgc
     3901 ccgccgcagc gcgagcagcc cgcggtagag ttccaggtgc ctggcgtgct cctgttcgtc
     3961 gaggctcgcc cagtccagct tgccccggac gaacgtctcc tcagcgcccg gatcggggat
     4021 gctgtcggcg tcctccgctt cgaagaaccc cgcaaactca gcgcggcgcc cctcggcaac
     4081 gagcggcgcg aggtcctgcc caaagtcgca gaagaacggg aacggggagt cggcgttcca
     4141 ttcctcgccc ataaaaagca tgggggtctg gggcgcgagc aggtagatct ctgccgccgc
     4201 gcgcacggct tctggtgagg cgagcttgcc tatcctctcc ccgaaggccc tgttgccgac
     4261 ctggtcgtgg ttctgcacga aagagacgaa gacgccgggc ggcaggctcg tatcaggcgc
     4321 ctctgcgtgg acgaaccccc cggacagggc cctcccgaga tccgccaccg gctccccggc
     4381 gtaatccgcg tagtaaccgg cgctctcccc cgtcatcact acgtgaagcg cgtggtgcag
     4441 gtcgtcgttc cactgcgcat cgtatggtcc acgcaggtag cgggcctcct tggcgtcgtt
     4501 ctccagaacc aggtgcacgt ggcggtggtg ccccggtcct tcccgcacgc gccccgcgag
     4561 ctcgtcaaga aagtgcgtct cggacgagtc tttgatggcg tgcacggcat cgagcctgag
     4621 gccgtcgaag ccgtactcct ccaaccagta gagggcgttg tggatgaagt actcgcgcac
     4681 gtgcccgctg ccctcgccgt cgaagttgac cgccgccccc caaggcgtgt cgtaccggtc
     4741 ggtgaagaac tgcggggcat accgagagag gtagttccct tcgggtccga agtggttgta
     4801 gacaacgtcc aggaagacca tcaggcccct gtcgtgcgcg ctctgcacga ggttctttag
     4861 ctcatccggg gttccgtagg cgctctccgg ggcgaaaggc aagaccccat cgtagcccca
     4921 gttgcggcgg cccgggaact cggagagcgg catcagctcg atcgccgtga cgccaagccc
     4981 gacgaggtgg tcgagccttc cctctacacc ggcgaaggtg cctaccggcg taaaggtgcc
     5041 gacatgcaac tcgtacagga ccacctcctc ccagggccgt ccacccggat accagtcccg
     5101 ccactcgtag gatacggggt cgacgaccgc gctcgcgccg tgcacgccgt cgggctgact
     5161 tctcgaggcg ggatccggaa cctcctcccc gccgtcgata cggaagcggt agcgtgtgcc
     5221 cgcacccgct ctggtggtca gggagaaagt gccgtcctcc tgtcgggaca tcgggatccc
     5281 gtcgccttcc aggacgaggt ccacgtcttc tgcggctggc gcccacaggt tgaacctcgt
     5341 ctcgccgtct tccagcacct cggcgccgag gggcatctcg tggcgccgcc tcaatccgcg
     5401 tcctcgtcga ccgtctcgcg cctacggtag ttcacgagca cggccatcga acggggtttc
     5461 agtttgtaga cgtcgtccgg cttgaggaag cgggcggccc tgagcccggc agcgtaggaa
     5521 gtgtccatga agacctccca gcgcacgtcc tcgggagagg ttggaaggcg gaagtcggcc
     5581 ccgttctcat cggcgttgaa gagcagcagg aagtcgtcgt cgacctgggg gcggccctgg
     5641 gcgtcgtgct cgccttcgag gaggccggac atgtggaggc cgagggagcg ggcgaaagag
     5701 ttgttccact cctcgtcggt catctcgcga ccctccgggg tcagccaggt gatgtccttg
     5761 gcgtctgcgc ccctaagctc gcggccctgg aaaaagctct tgcgccggaa ggccgggtgt
     5821 tcggtgcgga gccggatcat acgccgcacg aaggccagga aattcttgtc tggggcggag
     5881 aggttccagt cgagccagct cgtctcgttg tcctggcagt aggcgttgtt gttgccgccc
     5941 tgggttcggc gcatctcgtc cccggagagg atcatgggca cgccctgcga gagcaggagg
     6001 gtcgccatga tgttgcgcct ctggcgggcc ctggtgcgcc tgatctccag gtcctccgtc
     6061 tgcccttcga caccgtagtt acggctgagg ttgtggctct cgccgtcgtt gttgttctcc
     6121 ccgttggcct cgttgtgctt ccggtcgtag gagacgaggt ccgagagggt gaagccgtcg
     6181 tgggccgtga cgaagttgat gctcgcgtag ggcctgcgcc cgccgcgctc gtagagatcg
     6241 ctcgagccgg ttagcctgta ggcgagctcg cccacgatcc cgccgtcacc cttccagtag
     6301 gagcggacct cgtcgcggta tttgccgttc cactcggccc atcccaccgg gaagtttccg
     6361 acctggtagc cgccctcacc cacgtcccag ggctccgcta tgagcttggc ctgggagatg
     6421 acggggtcct gatggattat ggtgaagaag ctggagaggg catcgacctc gtagagctgg
     6481 cgcgccaggg cgctcgccag gtcgaatcta aagccgtcca cgtgcatctc cagtatccag
     6541 taccgcaggg agtccattat gagctgcaac acgcgcgggt gcatcatgtt gagcgtgttg
     6601 cccgtccccg tgaagtccat gtagtggcgc agatcatctg gcacgagcct gtagtaggcg
     6661 gcgttgtcga tgccgcgaaa ggagagcgtg ggccccagtc tattaccctc cgcggtgtgg
     6721 ttgtagacga cgtccaggat cacctcgagc cccgccgagt gcagccgctt gaccatggtc
     6781 ttgaactcgc tgatgctgcc cgacgccgca tagcgcatat ccggcgcgaa gaacccgata
     6841 gagttgtagc cccagtagtt cttgaggccc ctctcggcca ggtgcctgtc gtcaacgaaa
     6901 gcgtgaacgg gcatcagctc gacggccgtc accccgagct tcttgaggtg ctctatggcc
     6961 ggcgccgaag ccaggccggc gtaggtgcca cgcaagtcct ccggcacctc ggggtgcctc
     7021 tgggtgaacc ccttgacgtg gagctcgtag atgaccgtat cctcccacgg cgtgcgtggc
     7081 ggcctatcgt catcccaggt aaaggccgtg tcgaccaccc tgcacttggg catgccggcg
     7141 gcgttgtcct gccggtcgag ggcaaggtcc tcttcctcgg cgccgagcct gtacccgaag
     7201 tgggggtact cccagtccac agacccgacc gtcgccttgg cataggggtc gagcaggagt
     7261 ttggcagggt tgaagcgatg accgttttcg gggtcgtagg gcccgtggac cctgtagccg
     7321 tagagctggc cgagccggca gtagggcagg tagcagtgcc agatcctgtc cgtccactcc
     7381 ctgagctcga tcctctcgac ctcttccttc ccggaggcgt cgaagagaca gagctccacc
     7441 ccctcggcgt tctccgagaa cagggcgaag ttgacgccct gcccatccca ggtcgcgccc
     7501 agcggatagg gatgtccggg ccacacgacg ctggtcaagt acggttctcc acgttggcca
     7561 tgatgtcctt tcttcgaagg ccacaggcat caggtaatcc tcatgaggcc tgtgacctgt
     7621 agcctgatgc cttttcgcta gccgagcggc ttcagcacta ctgtcgccag cggcggtaac
     7681 gtgagcacga tggagtgctc tcggccgtgc gcctcgactc tttgtgtgct tatgcgacct
     7741 gtgttgccca cgccgctgcc gccgtagcgg gcatcgtcgg tgtttagggt ttcctcgtag
     7801 gcggccgctt cgggtacgcc tacgcggtag tcgtaacgca ctaccggcgt gaagttgcag
     7861 acgacgatcg ccagatcttc ctccgcgtcc gtgcgcctga gaaaggatac cacgctattc
     7921 tcgatatccc cgcccgagat ccactcgaac ccagcgggat cgaagtccac cctgtagagg
     7981 gccggggtgg tgcggtagag ggcgttcagg tcttttgtga gggcctggat gccctcgtga
     8041 gatggttcct ggagcaggtg ccagtcgagg gagatgtcgt ggttccattc ccggcgctgg
     8101 gcgatctcac cgcccatgaa gaggagttgt ttgccggggt gggcgtacat gaagccgtag
     8161 taggcgcgga ggttggcgaa ctgctgccat tcgtcgcccg gcatcttctc gagcagggac
     8221 cctttgccgt gcaccacctc gtcgtgcgag aggggaagga cgaagttctc accgaaagcg
     8281 taggcgaggg agaaggtgat ctcgttgtgg tggtacctgc ggttgatcgg gtcttcggac
     8341 atgtactgca ggctgtcgtg catccagccc atgttccact tgtagccgaa cccgagcccc
     8401 cccatggagg tcgggcggct gaccatgggc cacgcggtcg attcttcggc gagcgtggtc
     8461 gcgccgggat gccccccgaa gactacctcg ttcgcgcgcc gcaggaagga gacggcctcc
     8521 aggttctcgt tgccgccgtg cacgttcggg atccactccc ccgggttacg cgagtagtcg
     8581 aggtagagca tggaggcgac ggcgtcgacc ctgaggccgt cgacgtggta ctcggagagc
     8641 cagaacaggg cgttggagag caggtagttc ctgacctcgg ggcggccgta gttgaagacg
     8701 agcgtgcccc agtcggggtg ccggccctgg cgcgggtcag cgtggtcgta gaggtgggtc
     8761 ccgtcgaaga gggcaaggcc gtgctgatcc tcggggaagt gcgcggggac ccagtcgatg
     8821 aacacgccta tcccctcccg gtggaaggcg tccaccaggt ccttgaagtc gtctggcgtg
     8881 ccgaaccggc tcgtcggggc gaagaggccg aggggctggt agccccacga gccgtcgaag
     8941 gggtgctcgg tgggcggcag gaactccacg tgggtgtagc ccatctcagc gacatagggc
     9001 accagctctt cggcgagctg gccgtaggta tagtaggagc cgtcggggtt cctcctccag
     9061 gagcccagat gaacctcgta gatcgccatc ggggcgtcga gcgcgttcgc accttcacgc
     9121 ccccgcatcc acgcctcgtc gttccactcg aagcgcgtgg ggtcgtgtac gacggaggcc
     9181 gtgcccggaa gcctctcggc acggaagccg aaagggtcgg ccttcagcgg caagagctcg
     9241 ccgttcgggc cgaggatctc gtacttgtag acctcaccgt cgcccacgcc ggggagaaag
     9301 atctcccaga cgcccccagg atgcttgcgc atggggtgtt tgcgcccatc ccagaagttg
     9361 aagttgccga ccaccgagac acgcttcgcg ttcggggccc agaccgcgaa gctcgtgccg
     9421 gcgacgcctt ccagctccgt cgggtgtgcc cccagcttct cgtagagtcc gaggtgggtt
     9481 ccttcgccga acaggaagag gtcctgatct ccgagaaccc cggggaatcg gtaggggtcc
     9541 tcgacctcgt cctcgccgcc ttcctcccat ctgacccgca gacggtaagg cacgctctgc
     9601 cggacagccc cagcgaagaa gccatccggg tggatgcgct ccaggacgcc cacggcgtcg
     9661 ccttcgcggg agacgacctc gaccgacctc gcacccggca gaaacgcccg gaccacgggg
     9721 ccatcctccg aggcgtgcat gccgaggaag gagaacgggt ctccgtggtc tccggcgact
     9781 atggcggcga ccgcttccac ctgcctcata ggtcctcctc cccgtcctct agaaggtcca
     9841 ggacgctctt tatcgggatg ccgacccaat cgggacggtt gtccagctcg tagcggatct
     9901 cgtagagcgc cttctccagc gtgaagagcc cgatcagggc ccgcgcctgc tcctcgtctt
     9961 cggggtaaga ggcggcatcg cccacgcctt cccggtaacc gtcgaggaag gaccgaccgg
    10021 tccgttcctt ccagagcctg acccagggct cgagcgtttc gagctggtcg gcgcgatcgg
    10081 ccccgaggtc catcagcgcg gagcggatgg catagtcgaa cgagcggagc ataccagcca
    10141 catcccgcag gggcgagtgc ttcttgcgcc gctcggcaag gggcctcgcc ggctcgccct
    10201 cgaagtctat gatctcgaag tcgttgctga ccacgagtac ctgcccgagg tggtagtccc
    10261 cgtggtgcct ggtcttgacg accccgaaat tctgcccggc gacgctcttt acgctcctcc
    10321 tcaactcctc tcgcagaccg aggagccgct cgacgtcggg ggcgacttcg tcgggcaggc
    10381 tgctcccccg ccgcttcagc aggtcgaggg tgctccccgc ttccgcgaga accccgtcga
    10441 cccaccccgc cacctcttca ggggaggcca tctcgggggc gaaggcctca tcgtccgtcg
    10501 gggcggcaaa ggcggcatga agctctccgg tgcgcaggcc gagaatacga accaggctgg
    10561 cgaagaaggt gtcttcgggc gccagctctt catccggcgc gtcctcaccc tccgggctcc
    10621 acgaggtcag caggcg
//
LOCUS       DBNGVD010000150         6561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2763103, whole genome shotgun sequence.
ACCESSION   DBNGVD010000150 DBNGVD010000000
VERSION     DBNGVD010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2763103"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="AC16CM_04250"
     CDS             complement(<1..1832)
                     /locus_tag="AC16CM_04250"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase family protein"
                     /protein_id="HIR3139069.1"
                     /translation="MPRTLAWGFFVFLILLSVAIPAAAQNVPNAPTLSTTDRLDDRRF
                     VTTGDRAYEVGTEAGRYPAMGFHTRGEMGGVWSPPIKLLDGLWFGIGDRWIGPAEKFT
                     SGYGHVKMELPGRNGMTITRTDFVPDGRRSVLVGLTFTAGDSNRNFNLKMNAHSELMG
                     AYPWGETKPSQTTFNKQDGVSVRNDKLVFRERRSMENVPNALPHDWAAVVGSNLDLED
                     SETSAQTGEAYRGPQRDVICPASGPDAPPAPERCDDTAYGKGAGGQLVYNVKIAQNNT
                     KTVWFAVSGADFDGQNPDDARAAALQENANVLSDDPEALLEQKVEYRLGLRDMTRLDL
                     PGDRRLQRSIDWSKQNLADSVQTAKDLEIRRTRAGTVYPAPKGQLGRATFLGAGFPDY
                     QWLFGTDGEYTAFASVGVGQFGPIKDHLRALKRVSIIDNGDSGKVVHEVVTDGAVYFG
                     SNPDAGNTDETAKFPSAVALIWRWTGDDEFRDEMYGFSKRNMRYIFRELDEDRDGWPE
                     GLGNVEREGMGDEKLDNAVYTIRGLYDLADMARSKDDTATAQWATEKAQDMKRRFEAA
                     WWFGREGATQYADSLRNPDNEKVFQRHWIGATPMEVELTNQQNRA"
     gene            complement(1976..3760)
                     /locus_tag="AC16CM_04255"
     CDS             complement(1976..3760)
                     /locus_tag="AC16CM_04255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3139070.1"
                     /translation="MARGDQLAYEFARYLKIRGAWSAGWSPDGKRVSFLTEITGVPQV
                     WEMAYGGPSRPEQLTFYEERISSAEYSPTEDEMVFGMDAGGNERSQLFLLRDGEVTDL
                     TGDPEAIHYPGGFSPDGRRIAFTATRRNGTDFDVFVQDVSGGEPETAWEVSGYHTVAG
                     WAPDGGALIVSRHHSNVNNDLYRLDLGTGEATLLTPHEGDARFSGACMTPDGENLYLA
                     TDRDGEFLRLARLHLVTLELRYLTPDDWDVEGVELSRDGRYLVVSRNVDGYSDVLLFN
                     GRGVRMPDPQMPGGIVGGFRFSPDSKKLAFTLTAPERNPDVWVLDLVGGGARRLTRSS
                     TAGIPPGSFRKPRVVRYPSFDGREIPALFHEPEAESAPVIINVHGGPESQARPAFGPV
                     TQYLLGRGYAVFFPNVRGSTGYGKEYTHLDDVRLRMDSVRDLAHAAYWLGERGHERIA
                     VMGGSYGGFMVLSALTEYPDLWTAGVDIVGIANLVTFLENTGSYRRSLREPEYGSLDR
                     DREFLESISPIHKAENIEAPLMVVHGKNDPRVPVGEAEQIVDRVQRNGGTVQYLLYED
                     EGHGLAKLKNRLDAYPKIVSFLDAHLGA"
     gene            3847..4281
                     /locus_tag="AC16CM_04260"
     CDS             3847..4281
                     /locus_tag="AC16CM_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139071.1"
                     /translation="MVFRRLKSMFGGPTGSEDPPQPLYGLDESEREQIGTEADPLLDF
                     EAAMQRNEEAERAQQAGDTSLAIDLLEKSVAEGFVGSHPYERLASIYETRDPAEALRV
                     CEAFTKLAASGKMPRGAQRSADRKLPDFEARIDRYRGSLDKN"
     gene            4488..4850
                     /locus_tag="AC16CM_04265"
     CDS             4488..4850
                     /locus_tag="AC16CM_04265"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3139072.1"
                     /translation="MKSFLKTLLVAGALAVSLVAFQDDALADTQLASWYGPGLEGNYT
                     ASGDIFEPYTEYTAASPYLPFGTELLVTYGGASVVVEVTDRGPYVGGREIDLSAAAAD
                     AIGLTSAGVDYVDVQVLN"
     gene            5113..5634
                     /locus_tag="AC16CM_04270"
     CDS             5113..5634
                     /locus_tag="AC16CM_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3139073.1"
                     /translation="MEDGLQGVRFFEFLTEEEREEFAEAAEPVSFEAGENIIEEGAGQ
                     GSLFVLASGSVEVWKTLSGGGSRLLAEIDASRGRAVVGERGLLGESGASATVRAVSRV
                     EAVKIPRETFVSMVEEGRPAAFKLSYRIARTLARRLTRLDEEVVEAIRELESRGETDV
                     EAFRDRLITDWSV"
     gene            5644..6156
                     /locus_tag="AC16CM_04275"
     CDS             5644..6156
                     /locus_tag="AC16CM_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3139074.1"
                     /translation="MPDLKNVPIFKHLDADERAEFEELLEPASFRAGQMIFEEGAPEE
                     RLYVITSGTAEVYKTILPGRRQRLATIEAPTVVGEMGLLTEPRAAASVEATTRVEAHG
                     VGRDRLLEMLDADSPAACKLVYEIGRTLAERMAHTNATVAEVIARLENTRGYEDVDVF
                     RDQLVQDWGF"
     gene            complement(6175..>6561)
                     /locus_tag="AC16CM_04280"
     CDS             complement(6175..>6561)
                     /locus_tag="AC16CM_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139075.1"
                     /translation="IEFQFSAPIESSNGFVVSDLPYFKYGLDFLPPQGEITCYWDELD
                     SLLPYLESKNLKDGIHVTVRYRDLSDAQYQSQWRPNPFLYKDTRIVRRKGMGDLVNAV
                     ERMARSNDRGSSGQTLFPNPPSDDDM"
BASE COUNT         1136 a         2081 c         2135 g         1209 t
ORIGIN      
        1 gccctgttct gctggttcgt aagctcgacc tccatggggg tagccccgat ccagtgacgc
       61 tggaacacct tctcgttgtc cgggttgcgc agcgagtcgg cgtactgcgt ggcgccctcg
      121 cggccgaacc accaggcggc ctcgaagcgc cgcttcatgt cctgcgcctt ctccgtggcc
      181 cactgggctg ttgccgtgtc gtccttgctc ctcgccatgt cggcgagatc gtagaggcct
      241 cgtatggtgt agacggcgtt gtcgagcttc tcgtctccca tgccctcgcg ctccacgttg
      301 ccgagccctt cgggccagcc gtccctgtcc tcgtccagct cgcggaagat gtagcgcatg
      361 ttcctcttcg agaagccgta catctcgtcc cggaactcgt cgtcgccggt ccagcgccag
      421 atcagggcca ccgcggaggg gaacttggcc gtctcgtcgg tgttgcccgc gtcggggttt
      481 gacccgaagt acacggcccc gtcggtgacg acctcgtgca ccaccttgcc ggagtcgccg
      541 ttgtctatga tgctgacccg cttgagcgcc cgcaggtggt cttttatggg accgaactgc
      601 ccgaccccca cgctggcgaa cgctgtgtac tcgccgtcgg tgccgaagag ccactggtag
      661 tcggggaagc cggcgccgag gaaggttgct cgacctaact gccccttggg cgccggatag
      721 accgtgcctg ccctggtcct gcggatctcg aggtccttcg ccgtctgcac ggagtcggcg
      781 aggttctgct tgctccagtc tatggacctc tgcagcctgc ggtctcctgg aaggtcgagc
      841 ctggtcatat cgcgcaaccc gagcctgtac tcgaccttct gctcgagcaa ggcctcaggg
      901 tcatcggaga gtacgtttgc gttctcctgg agggcggcag ccctggcatc gtccgggttt
      961 tggccgtcga agtccgcgcc ggagaccgcg aaccacacgg tcttcgtgtt gttttgggcg
     1021 atcttcacgt tgtagacgag ctgtccgccc gcgcccttac cgtaagcggt gtcgtcgcaa
     1081 cgctccgggg ccggcggcgc gtcgggaccc gaggccgggc agatgacgtc cctctgcggt
     1141 ccccggtaag cctcgcccgt ctgtgcgctc gtctcgctgt cttcgaggtc gaggttggag
     1201 ccgacgacgg cggcccagtc gtggggcagg gcgttgggca cgttttccat gctcctccgc
     1261 tcccgaaaca cgagcttgtc gttccgcacg cttacgccat cttgcttgtt gaacgtggtc
     1321 tgggagggtt tggtctctcc ccacgggtag gcgcccatca gctccgaatg ggcgttcatc
     1381 ttgaggttga agttcctgtt ggaatctcca gccgtaaacg tcaggccgac gagcaccgaa
     1441 cgccgcccgt cggggacgaa gtccgtgcgg gtgatcgtca tgccgttcct accaggcaac
     1501 tccatcttca cgtgaccgta gccgctggtg aacttttctg ccggcccgat ccacctatcc
     1561 ccgataccga accagaggcc gtcgaggagt tttatcgggg ggctccagac gccgcccatc
     1621 tccccgcggg tgtggaaccc catggccgga tagcgtcccg cctctgtgcc gacctcgtag
     1681 gccctgtccc ccgtggtcac gaagcggcgg tcgtcgagcc tgtccgtggt cgagagcgtc
     1741 ggtgcgttcg gtacgttctg ggccgccgcg ggtatggcaa cggagagcag gatcaggaac
     1801 acgaagaaac cccaagccag cgtacgcggc atgcgtctgc ttgtttgaga gggtggagac
     1861 cacgggttat cgcaggacct tacagacaca cgcacgaatt ttccccttcc tgtaaacgtc
     1921 tcgtgaacgt gacacaaagc ttacgttttc gcatgaccag cgtcaacaga cgggatcagg
     1981 ctcccagatg cgcgtccagg aaggagacta tcttcgggta ggcgtctagc cggttcttga
     2041 gcttggcgag gccgtggccc tcgtcttcgt agagcaggta ctggacggtg ccgccgttcc
     2101 tctgcacccg gtccactatc tgctccgcct ccccaaccgg cacccgcgga tcgtttttgc
     2161 cgtggacgac catgagcggg gcctctatgt tctcggcctt gtggatgggg cttatggact
     2221 ccagaaactc gcggtccctg tcgagcgagc cgtactcggg ctcgcgcagg gaccggcggt
     2281 agctgccggt gttctccaga aaggtgacga ggttggcgat gcccacgatg tccaccccgg
     2341 ctgtccagag gtcggggtac tccgtgagcg cggagagaac catgaacccg ccgtaggagc
     2401 cgcccatgac agcgatcctc tcatggcccc gctccccgag ccagtacgcc gcgtgcgcga
     2461 gatccctgac cgaatccatc cgcaacctga cgtcgtcgag gtgggtgtac tccttgccgt
     2521 acccggtaga acctcgcacg ttggggaaga acacggcgta gcctctgcca aggaggtact
     2581 gcgtcacggg gccgaacgcc gggcgcgcct ggctctccgg accgccgtgc acgttgatga
     2641 tcacgggcgc gctctccgcc tccggctcat gaaagagggc cggtatctca cggccgtcga
     2701 agctcgggta acgcacgacg cgcggtttgc ggaagctacc aggcgggatg cccgcggtcg
     2761 aggaccgggt gaggcgtcgc gccccgccgc ctacgaggtc aaggacccac acgtcggggt
     2821 tgcgctcggg cgcggtgagg gtaaaggcga gcttcttcga gtcgggcgag aaccggaagc
     2881 cgcccacgat cccacccggc atctgcgggt cgggcatcct gacccccctg ccgttgaaga
     2941 gcagcacgtc ggagtagccg tccacgttgc ggcttacgac gaggtagcgt ccgtccctgg
     3001 atagctcgac accttccacg tcccagtcgt caggggtgag ataccggagc tccaacgtca
     3061 cgagatggag ccgcgcgagg cgcaagaact ccccgtcccg gtccgttgcc aggtacaggt
     3121 tctcaccgtc cggcgtcatg caggctcccg agaaccgcgc gtcgccctcg tgcggggtca
     3181 ggagcgttgc ctcaccggtc ccaaggtcca acctgtagag gtcgttgttc acgttggagt
     3241 ggtggcggga gacgatgagg gccccgccgt cgggcgccca gccagccacg gtatggtagc
     3301 cggagacctc ccacgcggtc tccggctcgc cgccggagac gtcctgcacg aaaacgtcga
     3361 agtccgttcc gttccgccgg gtcgccgtga aggcgatgcg cctaccgtcg ggtgagaacc
     3421 cccccgggta gtgaattgcc tcggggtcgc ccgtaagatc cgtgacctcg ccgtccctga
     3481 gcaggaacag ctgcgagcgc tcgttgccgc cggcgtccat gccgaaaacc atctcgtcct
     3541 ccgttggcga gtactccgcg gacgagatgc gctcctcgta gaacgtcaac tgctcgggcc
     3601 gggaaggacc cccgtaagcc atctcccaga cctggggcac gccggtgatc tcggtcagga
     3661 aagataccct cttcccgtcc ggcgaccacc ccgcgctcca ggcgcccctg atcttcagat
     3721 acctggcgaa ctcgtaagcc aactgatccc ccctcgccaa cgtgaccctc cggacaggtc
     3781 agcccgtgga gagggccgac cgacgtgctc ggatatactt gttcgagtat atccggaggg
     3841 agagacttgg ttttcaggcg cctgaagagc atgttcggag gaccaacggg gagcgaagat
     3901 ccgccgcagc ccctgtacgg cctggatgag agtgaaaggg agcagatagg caccgaggcc
     3961 gacccgctcc tcgacttcga agccgccatg caacgcaacg aggaggcgga gcgagcccaa
     4021 caggcgggag ataccagcct ggccatcgac ctgctcgaaa agagcgtcgc agaagggttc
     4081 gtcggtagcc acccctacga gcgactggcc tccatctacg aaacccgcga cccggccgag
     4141 gccctgcggg tgtgcgaggc tttcacaaag ctcgccgcta gcggcaagat gccccgcggt
     4201 gcccaacgat ccgccgaccg caaactgccc gacttcgagg cccgcatcga tcgctacagg
     4261 ggctccctcg acaagaacta gcaacacaac ccaacacccg taaaagccct gtgtaagcgg
     4321 ggtgtgagcc atcgacgtcc cgggcgtaac atctccgtaa cacgctctta accctgatgt
     4381 aatacgttca caaacaggcg gtaagaacaa cttgtagcgc cttgtaactc gcgcgtgaca
     4441 tagtaaggtt ctgggcttgt tgtgcaagag atctagaggg ggactccatg aagtcttttc
     4501 tcaaaacgct tctggttgcc ggcgcgcttg ctgtgtcgct ggtcgctttt caggatgatg
     4561 ccctggccga cacccagctg gcgagctggt acgggcctgg gctagagggc aactacacgg
     4621 cgagtgggga catcttcgag ccctacaccg agtacacggc ggcctcgccg tacctgccct
     4681 tcggcacgga gttgctcgtg acctacggtg gcgcttccgt tgtcgtcgag gtcaccgacc
     4741 gtggcccgta cgtgggcggt agggagatcg acctctctgc ggctgcggcc gacgctatag
     4801 ggctaacctc cgccggcgtc gactacgttg acgtgcaggt actcaactag agcggtccgc
     4861 gaagaggcag acctcttcgc aagctgtcag ctaaaagcct tcagcttctg gctgacctcc
     4921 cagagagcct caccggatct ggggcgagac acgagagcgg gcgtggcttt gatgccgcgt
     4981 ccgctttttt ctagctacca gtcctcagcg atcggccgaa gaaaaagccg accgctgacg
     5041 actgatagct gacggctcca catacgcata taatcttgac gaggaggttt agagacaagg
     5101 ggacggagaa gcgtggaaga cgggttacag ggggtcaggt tcttcgagtt tctgaccgag
     5161 gaggagcggg aggagttcgc cgaagcggct gagcccgtat cgttcgaggc tggcgagaac
     5221 attatcgagg agggggccgg gcagggtagc ctgttcgtgc tcgcctccgg gtcggtcgag
     5281 gtctggaaga ccctctccgg gggtggcagc aggctactcg cagagatcga cgcctcgcgc
     5341 gggcgtgccg tcgtggggga gagggggctg ctcggggaga gcggggcctc cgccaccgtc
     5401 agggccgtga gcagggtcga ggcggtcaag atcccgcgcg agaccttcgt gagcatggtc
     5461 gaggagggac ggcctgccgc cttcaagctc tcgtaccgca tagcgcgcac gcttgccagg
     5521 aggctcacgc gcctcgacga ggaggtcgtc gaggcaataa gggagctgga gagccggggt
     5581 gagaccgacg tcgaggcctt ccgggaccgt ttgatcacgg actggtccgt gtaaggagtt
     5641 ttgatgccag acctgaagaa cgttcctatc ttcaagcacc tggatgcaga cgagcgggcc
     5701 gagttcgagg agctcctcga gccggcgagc ttccgggccg ggcagatgat cttcgaggag
     5761 ggcgccccag aggagcggct ctacgttatc acctcgggaa cagccgaggt ctacaagacc
     5821 atacttcccg gacgcaggca gcggcttgcc accatcgagg cgccgaccgt ggtgggggag
     5881 atgggcctcc ttacggagcc ccgcgccgcc gcgagcgtcg aggcaaccac cagggtcgag
     5941 gcgcacggcg tgggcaggga tcgtctgctt gagatgctag acgccgactc cccggcggcc
     6001 tgcaagctcg tctacgagat cggccgcacc ctggctgaac gtatggccca taccaacgcg
     6061 accgtcgccg aggtcatcgc ccgcctggag aacaccaggg gatacgaaga cgtggacgtg
     6121 tttcgcgacc agctcgtgca agattgggga ttctagggcg gttcgcgaac cgccctacat
     6181 gtcgtcgtcg gagggtgggt tcggaaacag ggtctggccc gaggagcctc tgtcgttgct
     6241 gcgcgccatc ctctcgacgg cgtttaccag gtctcccatg cccttgcgcc tcacgatgcg
     6301 ggtgtccttg tagagaaagg ggtttggcct ccactggctc tggtactggg cgtccgagag
     6361 gtccctgtac ctgacggtga cgtggatgcc gtccttgagg ttcttcgact ccaggtaggg
     6421 cagcagggaa tcaagctcgt cccagtagca ggtgatctca ccctgcggcg gcaggaaatc
     6481 gagcccgtat ttgaagtagg gcaggtccga gaccacgaag ccgttggagc tctcgatggg
     6541 cgcggagaac tggaactcta t
//
LOCUS       DBNGVD010000151         4665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2946383, whole genome shotgun sequence.
ACCESSION   DBNGVD010000151 DBNGVD010000000
VERSION     DBNGVD010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2946383"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC16CM_04285"
     CDS             complement(<1..556)
                     /locus_tag="AC16CM_04285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139076.1"
                     /translation="MAESTKVAVLGTGIMGAAMARNLLAEGLEVRAWNRSREKAEPLG
                     EDGAEVVDSPADAARGADFLLTMLADADVVEEAVAGDVLPALAEGGVWLQMSTVGEKG
                     TRRLAETASEHGVDFVDAPVLGTRQPAEQGQLVVLASGPEEARERCQPVFDAVGNKTV
                     WLGEAGGGSRLKLVINNWIVGLLGV"
     gene            667..1620
                     /locus_tag="AC16CM_04290"
     CDS             667..1620
                     /locus_tag="AC16CM_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139077.1"
                     /translation="MGDNVAPALQAENVGRLYKSSGRGVSDVSFSVAAGEVYGLMGPN
                     GSGKSTLLRVLSTAMAPDSGNFRVGGTDGLKERRKVRARLGLMMDRPTHYNDLTGMAN
                     AYFFARAYGMEEDKAEGVLAELFDYFDLAEYKDDKVKTYSYGMGKKLALIEALAHGPE
                     VLLLDEPSLGLDYTSRLAYQRRIGELARDGVAVLLASNQVDEVESLCDRAAFLHRGRI
                     VAEGTPGELISSVRGVTRIVATLRNPVEYGSVAGVDGVQRVVPEGSDLVFHCDERPGV
                     VADVVGGIVGAGGDIVNLSVQRPNLEDVFVKLTGEALRDEV"
     gene            1610..2332
                     /locus_tag="AC16CM_04295"
     CDS             1610..2332
                     /locus_tag="AC16CM_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139078.1"
                     /translation="MRFDRPDAYWKKDVALFFSRRFALFLKMLYPLAIIVPVAASGMP
                     AKYAAAVIGIVAVMAGTFGAGESLTIDLNDGILVRVALTPLSPYRIVFEVLAVNAVLD
                     LLQLLPALLVVYLLHPVSLVWALAATLAVFATLVTANCIGIFIANFTSSPADVLLYAT
                     AILFPLIYISGFFRNQHPTGIMETLRGFVPFAYTDDALKTVFGLSTGTTALEILLYPT
                     IICAVLAFAVCLSAPRLLSQRQ"
     gene            2514..4643
                     /locus_tag="AC16CM_04300"
     CDS             2514..4643
                     /locus_tag="AC16CM_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3139079.1"
                     /translation="MNSNLRRYGIWVVIALVGAVCWGVLALSRGETINAGWLLFAALA
                     SYAIAYRFYARFIQTKVLGVDDSRATPAERLNNGRDFEPMDRRVLFGHHFAAIAGAGP
                     LVGPVLAAQMGFLPGTVWIIVGVILAGAVQDMTTLFFSMRRDGKSLGQMAREEIGPFG
                     GAAALIAVLSIMVILLAVLALIVVLALAESPWGSFSLAMTIPIAVFMGIYLRFLRPGR
                     VLEVSIIGVTLLFLAIIGGGWISESSSYAGFWTFSPNQIVFALIAYGFIASVLPVWIL
                     LAPRDYLSTFMKIGTIGLLAVGIALTLPTMQMPRTTEFAFNGQGPVFAGALFPFVFIT
                     IACGALSGFHSLIASGTTPKLIQKESQVRMIGYGAMLTESFVAVMALIAACILDPGVY
                     FAMNANPALLGDTAQSAAQAVSGFGFSVNAETLTGTAAAVGEESIIGRTGGAPTLAVG
                     MSQILTGLTGFLGPSLQAFWYHFAIMFEALFILTTVDAGTRVGRFMLQDLIGNVYRPF
                     ARTSWLPGNIIASALIVAGWGYFLYAGVNDPLGGINQLFPLFGIANQLLAAVALTIGT
                     TILIKMGKLRYAWVTALPLAWDAAVTLTASWQKIFSADPAIGFFAQRAAAAQSLQAGE
                     LNDTMIALKAQSLDDARQILINTTVDGVLSILFAAMIIIVIADAARVWYGLIWGGKQP
                     EMSEAPWEESNLDAEGKEIERETVGAR"
BASE COUNT          719 a         1603 c         1457 g          886 t
ORIGIN      
        1 gcacgccgag aaggccgacg atccagttgt ttatgacgag cttgagtcgg ctgccgcccc
       61 cggcctcacc gagccacacg gtcttgttgc cgacggcgtc gaagacgggc tggcacctct
      121 ctcttgcttc ctcggggccg gaggcgagca ccactagctg gccctgctcc gcgggctgtc
      181 tggtcccgag aacgggcgcg tcgacgaagt ccacgccgtg ctcgctggcc gtctcggcga
      241 gcctcctggt gcctttctcc cccaccgtgc tcatctgaag ccacacgccg ccttcggcca
      301 gcgcggggag cacatcgccc gccacggctt cctccacgac gtcagcgtcg gcgagcatgg
      361 tcagtaggaa atcggcgccg cgcgcggcat cggccgggct gtcgacaacc tcggcgccgt
      421 cttccccgag cggctcggcc ttctcgcgcg agcggttcca ggcccgcacc tccagaccct
      481 cggcgaggag gttgcgtgcc atcgcggcgc ccatgatccc cgttccaaga accgccacct
      541 tcgtgctctc agccataggt cctccagtct tcgaaccatc cgtacttacc gtaccccgcc
      601 acggtgcaaa cactcggtga tgcccgccac aacccgtcca ggggggcggc gctacaattc
      661 ccgtatatgg gagataacgt cgcgccagcc ctccaagccg agaacgtagg ccgtctgtac
      721 aagagcagcg ggcgcggggt ctcggacgtg agcttctccg tcgccgccgg cgaggtctac
      781 ggcctgatgg gtccgaacgg ctcgggaaag agcacgctgt tgcgcgtgct ctccaccgcc
      841 atggccccgg attcaggcaa cttccgcgtg ggcgggaccg atggcctgaa ggagaggcgg
      901 aaggtgaggg cgcggctcgg gttgatgatg gaccgtccca cccactacaa cgacctcacg
      961 gggatggcca acgcctactt cttcgcccgc gcctacggga tggaggagga caaggcggag
     1021 ggcgtgctcg ccgagctgtt cgactacttc gacctggccg agtacaaaga cgacaaggtc
     1081 aagacgtact cctacgggat gggcaagaag ctggccctga tcgaagccct cgcgcacggc
     1141 ccggaggttc tgctccttga cgagccctcg ctcgggctcg attacacctc gcggctcgcc
     1201 taccagcgca ggatcgggga gctggccagg gacggcgtcg cggtcctgct cgcgagcaac
     1261 caggtcgacg aggtcgagtc cctgtgcgac cgggccgcct tcctgcaccg cggcaggatc
     1321 gtcgccgagg ggacgcccgg ggagttgatc tcctccgtgc ggggcgtgac gcgcatcgtg
     1381 gccaccctgc gcaaccccgt ggagtacggt tccgttgcgg gggtagacgg cgtccagcgc
     1441 gttgttccgg aggggagcga cctggtcttt cactgcgacg agcgtcctgg cgtggtcgcc
     1501 gacgtcgtcg ggggcatcgt cggcgccggc ggggacatcg tgaacctctc ggtacagagg
     1561 ccgaacctgg aggacgtgtt cgtcaagctc acgggggagg cgttgcgcga tgaggtttga
     1621 ccgccccgac gcctactgga agaaggacgt ggcgttgttc ttctcccgga ggttcgcgct
     1681 gttcttgaag atgctctacc ccctggccat catcgtgccg gtggcggcga gcggcatgcc
     1741 cgccaagtac gccgccgccg tgataggcat cgtggccgtg atggccggga ccttcggggc
     1801 tggtgagagc ctgaccatag acctgaacga cggcatcctg gtgcgcgtcg cccttacgcc
     1861 gctctcccct tacaggatcg tcttcgaggt gctcgccgtc aacgccgtgc tcgacctgct
     1921 gcagttgctc cctgcgctcc tggtcgtcta cctgcttcac cccgtctcgc tagtctgggc
     1981 gctcgcggca accctggccg tctttgccac gcttgtcacg gccaactgca tcggcatctt
     2041 tatcgccaac ttcacctcct cccccgccga cgtgctgctg tacgctacag ccatcctctt
     2101 tcccctgatc tacatctccg gcttcttccg aaaccagcac ccgaccggca tcatggagac
     2161 gcttcgtggc ttcgtcccct tcgcctacac ggacgacgcc ctgaagaccg tcttcggcct
     2221 gagcacgggc acgaccgcgc tggagatcct gctgtatccc accatcatct gcgccgtcct
     2281 ggccttcgcc gtctgcctct ccgccccgcg cctgctctcc caacgccagt agcgtaaggt
     2341 cgcggcccgg agcgagaggg ggcgcttcgc gaagcgcccc tacatttcct acgtgtttcg
     2401 ggtgtccggc ccggagttgg cgatcccttg cccttgatct ccgcccgtta tgccaatctt
     2461 cgtggatttt ccgttattat gggcgcaggt tcttgggcaa gggtggaggg atcatgaata
     2521 gcaatcttcg tcggtatggt atctgggtcg tcatcgcgct cgttggggct gtgtgttggg
     2581 gggtgctggc actctcacgg ggggagacga tcaacgccgg gtggctgttg tttgcggcgc
     2641 tggcgtccta cgccatcgcc tacaggttct acgcccgctt catacagacc aaggtgctcg
     2701 gggttgatga ctcgcgggcc acgccggctg agcggctcaa caacggccgc gacttcgagc
     2761 cgatggaccg gagggtgctg ttcgggcacc acttcgccgc catagcggga gccgggccgc
     2821 tcgtcgggcc cgtgctcgcg gcacagatgg ggttcctgcc ggggaccgtc tggatcatcg
     2881 tcggcgtcat cctggctggc gccgtgcagg acatgaccac gctcttcttc tccatgcgca
     2941 gggacggcaa gtcgctgggc cagatggcga gggaggagat aggaccattc ggtggggctg
     3001 cggcgctcat cgccgtcctg tccatcatgg tcatcctgct cgccgtcctg gcgctcatcg
     3061 tcgtcctcgc gctcgccgag tccccgtggg gttcgttctc gctcgccatg accatcccca
     3121 tcgcggtgtt catgggcata tatctgcggt tcctgagacc aggacgggtg ctcgaggtct
     3181 cgatcatcgg ggttacgcta ttgttcctgg cgatcatcgg cggtggctgg atctccgaat
     3241 cctcgtccta cgcgggcttc tggaccttca gccccaacca gatagtcttc gccctgatcg
     3301 cctacggctt tatagcctcg gtgttgccgg tatggatact tctcgccccc cgcgactacc
     3361 tctcgacgtt catgaagatc ggcaccatag gcctgctcgc ggtcggcatc gccctgaccc
     3421 tgccgaccat gcagatgccc cggaccacgg agttcgcctt caacggccag ggtccggtct
     3481 tcgccggcgc cctcttcccg ttcgtgttca tcacgatagc gtgcggggcg ctctcgggct
     3541 tccactcgct catcgcctct ggcacgacgc ccaagctcat ccagaaagag tcccaggtgc
     3601 gcatgatcgg ctacggggcc atgctcacgg aatctttcgt ggcggtaatg gccctgatag
     3661 ccgcgtgcat cctcgacccc ggcgtgtact tcgccatgaa cgccaacccg gcccttctcg
     3721 gcgacaccgc ccagtcggcg gcccaggccg tgagcggctt cgggttctcc gtcaacgccg
     3781 agacgcttac gggtacggcc gcggccgtcg gcgaagaatc catcatcggc aggaccggcg
     3841 gggcgcctac cctcgcggtg gggatgagcc agatcctcac cggcctgacc ggcttcctcg
     3901 ggccttcact gcaggcgttc tggtaccact tcgccatcat gttcgaggct ttgttcatcc
     3961 tgacgaccgt ggacgccgga accagggttg gaaggttcat gctccaggac ctgatcggta
     4021 acgtgtaccg tcccttcgcc cgtacctcct ggctgccggg caacatcata gccagcgccc
     4081 tgatcgtcgc cgggtggggc tacttcctct acgccggcgt caacgacccg cttggcggca
     4141 tcaaccagct cttcccgctc ttcggcatcg ccaaccagct actggccgcc gtcgccctga
     4201 ccatcggcac gaccatcctc ataaagatgg gcaagctccg ctacgcctgg gtaacggcgc
     4261 tgccgctcgc gtgggacgcg gcggtaacgc tcacggcaag ctggcagaag atcttctccg
     4321 ccgaccccgc cataggcttc ttcgcccagc gggccgccgc cgcccagtcc ctacaggcgg
     4381 gcgagctgaa cgacaccatg atcgccctca aggcacagag ccttgacgat gcaaggcaga
     4441 tcctgatcaa caccaccgtg gacggcgtgc tctccatact gtttgcggcc atgatcatca
     4501 tcgtgatcgc cgacgccgcc cgcgtgtggt acggcctgat ctggggcggc aagcagcccg
     4561 agatgagcga ggcgccctgg gaggaatcca acctcgacgc cgagggcaag gagatcgaac
     4621 gcgagaccgt aggagccaga tagagaacga tgcgagagta cctgc
//
LOCUS       DBNGVD010000152         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_942917, whole genome shotgun sequence.
ACCESSION   DBNGVD010000152 DBNGVD010000000
VERSION     DBNGVD010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_942917"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC16CM_04305"
     CDS             complement(<1..652)
                     /locus_tag="AC16CM_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3139080.1"
                     /translation="MLEVAIMVEGQEGIGWDRWRRIARTVEDAGYAGLYRSDHFPYET
                     GSYQDALELWTSLTWLADNTRRIEFGPLVSPVSFRHPVIIAWQASAVDNLAGGRLRLG
                     LGAGWNEEEHGAFGFDLLDTDSRFERFEEGLEVVSRLLRSEKPVSYAGEYYNLRDALI
                     VPRSPRPGGIPIVIGGNGPRRTLPLAARYADEWNAVYLTPEEFADLDSRLDGLTREA"
     gene            1036..>1720
                     /locus_tag="AC16CM_04310"
     CDS             1036..>1720
                     /locus_tag="AC16CM_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3139081.1"
                     /translation="MLHTIFYGVLVVGGVCLLALVGFELVHRLVPASARQQHNDVAGF
                     IYAALGVIYAVLLALVVIAVWEEYGAASDTVEREANAAADIYWLADQLPEDRGNAVQE
                     LVHSYVEEVVYTEWPLMQRREQPPLEREGDAPTGWSIIDDIRHNIQGFEVRTQSEEQL
                     YAEGLDQVDTLSDARRMRLVASEEGVPGVLWSVLIFGGIAAVSFTYLFGLESTWAHRL
                     MVVTLAAVIG"
BASE COUNT          271 a          550 c          592 g          307 t
ORIGIN      
        1 cggcctcccg cgtcagcccg tccagacggg agtcgagatc cgcgaactct tccggtgtga
       61 ggtagacggc gttccactcg tcggcgtagc gggccgcgag cggcagcgtt ctgcgggggc
      121 cgttgccacc gatcacgatg ggtatcccgc cgggccgcgg cgagcggggc acgatcaggg
      181 catcgcgaag gttgtagtac tcgccggcgt aggagactgg cttttcgctc ctcagcagcc
      241 ggctcacgac ctcaagcccc tcttcgaagc gctcgaagcg gctatcggtg tcgagaaggt
      301 cgaagccgaa ggccccgtgc tcttcctcgt tccagccggc gcccaggccg agccgcaggc
      361 gtcccccggc caggttgtcg acggctgagg cctgccaggc gatgataacg ggatgacgaa
      421 acgacacggg cgagaccagc ggaccgaact cgatcctcct ggtgttgtcc gccagccagg
      481 tgagcgaggt ccagagctcc agggcatcct ggtaagagcc cgtctcgtac gggaagtggt
      541 cggaacggta caggccggcg tagcccgcat cctcgaccgt gcgtgcgatc cgccgccagc
      601 gatcccaccc gataccctcc tgcccctcta ccatgatcgc gacttcgagc acgagacttc
      661 ctttctccca cggctgggga cctcacgaca ggatcgaccc ctgcatacct ggtggttcgt
      721 agagattccc gcccctgcac cgctaccagg ataacggtac acgaagtcgg agacgcccgc
      781 agcgaccggg tctgccgccc ggacggccct gctgtggcgt tctctttctc gaatgctctg
      841 tagggatcgt gccgtgtcag cgagccgagc gtgaggttgg gttaacgggt gcagggcctc
      901 ccccacgagc ttctggtctc ctgacgttcc ggcggtttcg gccgggcggc gtagttcgcc
      961 cgggacgtag cgtggtgcct gtggtggcgg ctagaataac ggccagtcgg cctgatcagg
     1021 gaggggtgtc tcgccttgct gcacacgatt ttctatggcg ttctggtggt cgggggggtt
     1081 tgcttgctcg cgctcgttgg gttcgagctg gtgcaccgcc tcgtgcccgc ctccgcgcgc
     1141 cagcaacaca acgacgtggc cggcttcatc tacgcggcgc tcggtgtgat ctacgccgtg
     1201 ctactggcgc tcgtggtgat cgccgtgtgg gaggagtacg gggcggcaag cgacaccgtc
     1261 gagagggagg ccaacgccgc ggccgacatc tactggctgg ccgaccagct tccggaggac
     1321 cggggcaacg ccgtgcagga gctcgtccac tcctacgtcg aggaggtggt ctatacggag
     1381 tggccgctga tgcagcgcag ggaacagccc ccgctggagc gggagggaga tgccccgacc
     1441 gggtggtcca tcatcgacga catcaggcat aacatacagg gtttcgaggt gcgcacgcaa
     1501 tccgaggaac agctctacgc cgaggggctc gaccaggtcg acaccctctc cgacgccagg
     1561 aggatgcgcc tggtcgcctc tgaggagggg gtccccggag tgctctggtc ggtcctgatc
     1621 ttcgggggca ttgccgcggt tagctttacc tacctcttcg gcctggagag cacgtgggcg
     1681 cacaggctca tggtggtgac cctggcggct gtgatagggc
//
LOCUS       DBNGVD010000153         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3857420, whole genome shotgun sequence.
ACCESSION   DBNGVD010000153 DBNGVD010000000
VERSION     DBNGVD010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3857420"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="AC16CM_04315"
     CDS             <1..1527
                     /locus_tag="AC16CM_04315"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HIR3139082.1"
                     /translation="LVRGEVVDQFSTLVNPERPIDPFVVRLTGITDRMVADAPTIGEV
                     MPRFEEFVEGCVLVGHNVQFDCSFVTAARDGVKLPNEVLDTLKLARCLVPGLKRYRLS
                     ALVSHFGVRQTPNHRALADAAATTEVFKRLLKLLRSAGMGSVGDAAALRGGGGRIKPQ
                     KQHLASGLPNTPGVYYFVDKHGTTLYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVA
                     EVRIRETESELHALILEAREIKRLLPRFNSAGRGDKASWYIRLDTNEAYPVPERVSEN
                     VPDEGLIHLGPFRSAGTLDTCVEALGRIFPLKRCSGESGACFYGQMGRCAPCMGMGEE
                     EYRREVVDEVVALLRGEGGEDHLRALVAERQRLAADLEFEAAARLRDLITGIERVRLS
                     RAVVSADGVQAVVAPSTEPGMIEVFVLRSGRLISHRGFEPEDEKGLTRFASEVLARPA
                     PNGSAPGKEGSNEARIVAAYLRRRSTVVEAVQLREAGDLLKVAGSVVEAAGEGATSLA
                     "
     gene            1623..2321
                     /locus_tag="AC16CM_04320"
     CDS             1623..2321
                     /locus_tag="AC16CM_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139083.1"
                     /translation="MVQDLQGVTRKILLVDDEPSLQKMLTHALEREGFQVQVAGDGEA
                     ALEAFKSYEPHLIVLDIMLPKLDGTEVIRRIRSQSDVPVIMLTARDDEIDRVVGLELG
                     ADDYVTKPFAVRELVARVRAIMRRAAVPAGQRPDELTYDRLRINLPSRRVSADGEEVD
                     LTYTEFELLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNP
                     EFIHTARGVGYVFR"
     gene            2308..>3299
                     /locus_tag="AC16CM_04325"
     CDS             2308..>3299
                     /locus_tag="AC16CM_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3139084.1"
                     /translation="MFSARKRAEGRDPAPNPWHKARKLLARKDSFKPRVSMRIWLTAL
                     FVLVTAFAAVTAYQIVHPILRETLERSSAAAFNQVGVQFEEQVQRLNEQGEGLNEQRI
                     SAFARSHGLQWGIVDKDGSQVQGGNLDDWGVGTVERAFEEPRRPVGGKEPIKTGRYKG
                     QQRATYAYPIDVKVEGKDEYQPRAVVFVKYYTQSDIENVQSALNNIERLALLAGALAL
                     LIAGFAGYFAAVLISRRISRLGLAAENLAAGHFDERITSSVEDEVGSLGETFNAMAVS
                     LKGAFGQIEQEKERGSAILNGMTDAVIGVNRELEPVFLNPRAKRLLESSDHDFH"
BASE COUNT          612 a         1042 c         1133 g          512 t
ORIGIN      
        1 ctcgtgcgtg gggaggtggt ggaccagttc tccacgctcg tgaacccgga gaggcccata
       61 gaccccttcg tggtgcgcct gaccggcata acggaccgga tggtcgctga tgctcccacc
      121 ataggcgagg tgatgcctcg cttcgaggag ttcgtcgagg gatgcgtcct tgtcgggcat
      181 aacgttcagt tcgactgctc tttcgtcacc gccgcgaggg acggggtcaa gctccccaac
      241 gaggtactag acaccctgaa gctcgcccgc tgcctggtgc caggcttgaa gcgctaccgt
      301 ctcagcgcgc tcgtctccca cttcggggtg cgccagacgc cgaaccacag ggcgctcgcc
      361 gacgccgcgg cgaccaccga ggtgttcaag aggctgctca agctcctgcg ctccgccggc
      421 atgggcagcg tgggagacgc cgccgccctg cgcggcgggg gggggcgcat caaaccgcag
      481 aagcagcacc tcgcgagcgg gctcccgaac acgccgggcg tctactactt tgtggacaag
      541 catggcacca cgctctacgt cggcaaggcc aaagacctca aggcgcgcgt caggacctac
      601 ttcaacggcg gggacgggcg ccgcaaggtc gggcgcctcg tggaggaggt ggccgaggtg
      661 cgcatcaggg agacagagag cgagcttcac gccctcatcc tggaggcgag ggagataaag
      721 cgcctgctcc cacgcttcaa ctcggctggc aggggagaca aggcgagctg gtacatcagg
      781 ctcgacacaa acgaggccta ccctgttccc gagcgggtct ccgagaacgt tcccgatgag
      841 ggtctcatcc atctcggtcc cttcaggagc gccggcaccc tggacacctg cgtggaggcg
      901 ctgggcagga tcttcccgct caagagatgc tcgggtgaaa gcggggcctg cttctacggc
      961 cagatgggcc gctgcgcgcc gtgcatgggc atgggcgagg aggagtaccg tcgtgaggtc
     1021 gttgatgagg tcgtggccct gctgcgtggc gaggggggcg aggatcacct cagggcactc
     1081 gtcgcggagc ggcagcggct ggcggcggat ctcgagttcg aggcggctgc caggctcagg
     1141 gacctgatca ccggcatcga gcgggtgagg ttgtccaggg ccgtggtcag cgccgatggg
     1201 gtgcaggccg tcgtcgcccc ttccacagag cccggtatga tcgaggtctt cgtcctgcgg
     1261 tccgggcgcc tgatctcaca ccgcggcttc gagccagaag atgagaaggg cctgacgagg
     1321 ttcgccagcg aggttctggc caggcctgcg ccgaacgggt cggcaccagg caaggagggg
     1381 tccaacgagg cgaggatcgt cgccgcctac ctcaggcgtc gctcgacggt ggtcgaggcc
     1441 gtgcagttga gggaggcggg ggaccttctg aaggtggccg ggagcgtggt cgaggccgcg
     1501 ggagagggtg ccacgtcgct ggcgtaggag cccgcgttgc aggggggcta cccgccccat
     1561 catgctagaa taagggcatg cagtcgtttg ctcctccaga agagaagaag gtctagcagg
     1621 gtatggtgca ggatctacag ggcgtaaccc gcaagatcct cctcgtcgac gacgagcctt
     1681 cgctccagaa gatgctcacg cacgccctgg agcgcgaggg tttccaggta caggtcgcgg
     1741 gcgacggtga ggccgccctg gaggcgttca aatcttacga gccgcacctg atcgtgctcg
     1801 acatcatgct ccccaaactg gacggcacgg aggtcataag gaggatccgg tcccagagcg
     1861 acgtgcccgt aataatgctg accgcccgcg acgatgagat agacagggtc gtgggcctcg
     1921 agctcggcgc cgatgactat gtcaccaagc ccttcgccgt gagggagctc gtggcgcgcg
     1981 tgcgggcgat catgcgccgc gccgcggtac ccgcgggtca acgccccgac gagctgacct
     2041 acgacaggct caggatcaac ctccccagcc gcagggtgtc ggcggacggc gaggaggtgg
     2101 acctcaccta cacggagttc gagctcctgg tgacgcttgc ctccagcccc ggcagggtgt
     2161 tctcgcgcag cgctcttctg cgccgcgtgt ggggcgacga gttccgagac gaacgcacgg
     2221 tggacgtcca catccgccac ctccgcgaga agatagaacg cgacccgaga aaccccgagt
     2281 tcatccacac cgcgcggggt gtcggatatg ttttccgcta ggaagcgagc agagggccga
     2341 gaccccgccc ccaacccctg gcacaaggcc aggaagctgc tcgcccgcaa ggactccttc
     2401 aaaccgcgcg tcagcatgcg catctggctt acggccctct tcgtgcttgt taccgccttc
     2461 gccgcggtga cggcctacca gatcgttcat cccatcctcc gcgagaccct ggaacgttcg
     2521 agcgccgccg ccttcaacca ggtcggggtc cagttcgaag agcaggtgca gcggctgaac
     2581 gagcagggcg agggactcaa cgaacagcgc atcagcgcgt tcgcccgctc ccacgggctg
     2641 cagtggggca tagtggacaa agacggcagc caggtacagg gtgggaacct ggacgattgg
     2701 ggcgtcggga cggtggagcg cgccttcgag gagccaagga gaccggtagg cggaaaagaa
     2761 cctatcaaga cgggccgcta caagggccag cagcgggcaa cctacgccta tcccatcgac
     2821 gtgaaagtcg aggggaagga cgagtaccag cccagggccg tggtcttcgt caagtactac
     2881 acccagagcg acatcgagaa cgtgcagtcg gcgctcaaca acatcgaacg gttggccctg
     2941 ctcgccggcg cgctcgcgct ccttatcgcg gggttcgcgg gttacttcgc agccgtattg
     3001 atctcccgcc gcatcagccg cctcgggctg gccgccgaga acctggcggc gggccacttc
     3061 gacgagagga tcacctccag cgtggaagac gaggtgggct ccctcggcga gacgttcaac
     3121 gccatggccg tctccctcaa aggtgccttc gggcagatag agcaggagaa ggaacgcgga
     3181 agcgcgatcc tgaacggcat gaccgacgcc gtgatcggcg tgaacaggga gctggagcct
     3241 gtcttcctta acccccgcgc gaagaggttg ctcgagtcct ccgaccacga cttccacaa
//
LOCUS       DBNGVD010000154         3877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1489270, whole genome shotgun sequence.
ACCESSION   DBNGVD010000154 DBNGVD010000000
VERSION     DBNGVD010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1489270"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="AC16CM_04330"
     CDS             <1..1195
                     /locus_tag="AC16CM_04330"
                     /inference="COORDINATES: protein motif:HMM:NF018479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR3139085.1"
                     /translation="RFVRPQVMRNLASEQRAEERSSTWLELFFDLCFVVAVAALASGL
                     HHDPNLDGMLRFLGLFVPVWWSWMIFTWYATSFDNDDVPYRVTYFVAMLSMLGLAASV
                     GRIGVDPSAVVGFVLAYSLMRLLVAGLFVRAGRSVPAYLRPFVACYAAGNLIGATIWL
                     SSLLVPAPFQYALWALGLSVELLGPILAVMTLDNPGITFHPRHIAERYGLFTLIVLGE
                     SVLAVTSGTVGTDWAPTAVLTAVAGFVAAVSIWWLYFDHVRSSGIELGPRPAFYWGYG
                     HYAVYAGIAAFGVGVQLAIEAAAPLYALASGAPASEGYGLGARTVLAGGVILFLLGIA
                     FVDRINEGTQNDRVLIARLAAVTLLVPIIAFGSLLSPLTFTVTVCLVLLALTAYETLT
                     EDPPE"
     gene            complement(1244..1906)
                     /locus_tag="AC16CM_04335"
     CDS             complement(1244..1906)
                     /locus_tag="AC16CM_04335"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3139086.1"
                     /translation="MQSLLDFIGSIPRSPEGVLVFVTNLLVAHGYLVILFGAALDNFG
                     LPASGDIVLFAGGLFANGGELSLPLVMLSGFAGAFISDNSVYWIGRIGGRPMMYRILK
                     MRFLHFLINEKSLDKVGHYFETHGGKTVFVGRFGPGLRSMTPLFAGVSRMTYYRFLPY
                     NLSAVIVWAVAYSLVGYVFGQYWDELLAVARSLGYGVIALVVLLLIAYVLRRRWSGND
                     PH"
     regulatory      2217..2415
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2448..2657
                     /locus_tag="AC16CM_04340"
     CDS             2448..2657
                     /locus_tag="AC16CM_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139087.1"
                     /translation="MEKILGRSYRPVLPLWSLLVLALLLLALFVLLSASGELLLPLLG
                     QAAGPFDYLHEFAHDGRHLLGAPCH"
     gene            2666..3385
                     /locus_tag="AC16CM_04345"
     CDS             2666..3385
                     /locus_tag="AC16CM_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HIR3139088.1"
                     /translation="MVSAYLRRGMAAGLLVGLLIGLFAFFVGEPVLDRAIALEEASEG
                     SHHEDGAHHEEIFSRSTQKVGLFFATGLFGVTVGGVFGIGYAFFRDRLAAGSDLGRSV
                     SLAGAIFVGPFLIPFLKYPANPPSVGDPSTIRERTAAYFALVALSLLTMLVVWLTART
                     LKARGFGAPRRRVMVGAGLVLVLAILFVSLPAGPSAEGFPSGLLWAFRFSSIGTQLVF
                     WAGLGLLFGWLCERARRKGTA"
     gene            3382..>3877
                     /locus_tag="AC16CM_04350"
     CDS             3382..>3877
                     /locus_tag="AC16CM_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR3139089.1"
                     /translation="MSGALLVAGTHSDAGKSVVVAGICRWLAREGIGVAPFKAQNMAL
                     NSFVTREGAEIGRAQAAQAAAAGVEPEAAMNPVLLKPSAERITQVVVRGRAWATASAA
                     SYGAMKRDLMPVVLESLGDLRRRFDVVICEGAGSPAEINLRKNDLANMGLARAARLPA
                     LLVGD"
BASE COUNT          609 a         1302 c         1244 g          722 t
ORIGIN      
        1 gcgcttcgtg cgccctcagg tcatgcggaa cctcgcgagc gagcaacgcg ccgaggagcg
       61 gagctccacc tggctggagc tcttcttcga cctttgcttc gtcgtggcgg tcgcggcgct
      121 ggcaagcggg ctgcaccacg atccgaacct agacgggatg ctccgtttcc tcggtctctt
      181 cgttcccgtc tggtggtcgt ggatgatctt cacctggtac gcgacctctt tcgacaacga
      241 cgacgtgccc taccgcgtaa cgtatttcgt cgcgatgctc tccatgctcg ggctcgccgc
      301 ctctgtgggc cggatcggcg tggacccctc cgccgtggtc ggtttcgtgt tggcttactc
      361 cctgatgcgc ctgctcgtgg ccggcctctt cgtgagggcc ggacgcagcg tgccggccta
      421 cctgcgcccc ttcgtggcct gctacgccgc agggaacctt atcggggcga ccatctggct
      481 ctcttcactc ctcgtgcccg cgcccttcca gtacgcgctc tgggccctcg gactttccgt
      541 ggaactactg ggcccgatcc tcgccgtgat gaccctggat aaccccggga tcaccttcca
      601 cccgcgccac attgcagagc gctacgggct gtttaccctg atcgtgctcg gcgagtccgt
      661 gctggcggta acctcgggaa cggtcggtac tgactgggcc ccgacggcgg ttctgaccgc
      721 tgtggcgggc ttcgtcgcag cggtgagcat ctggtggctg tacttcgacc acgttaggtc
      781 ttccggcatc gaacttggcc cgcgcccggc gttctactgg ggctacgggc actacgccgt
      841 ctacgctggc atcgccgcct tcggcgtggg cgttcaactg gccatcgagg ccgcggcccc
      901 actctacgcg ctggcctcgg gagcgccggc ctccgaaggt tacggtctgg gggccagaac
      961 ggtgctcgcg ggtggcgtca tcctcttcct tctaggcatc gctttcgtcg accggatcaa
     1021 cgaaggcacc cagaacgatc gcgtcctgat agcacgcctc gcagccgtaa cgctcctggt
     1081 cccgatcatc gccttcggct ctctgctctc gccgcttacc ttcaccgtca cggtctgcct
     1141 ggtcctgctc gccctgacgg cctacgaaac gctaaccgaa gacccaccag aatagggtga
     1201 gaggcatctt gtcgaaagcc gtggcggcag ttcgcgaatt gccctaatgt ggatcgttgc
     1261 ccgaccagcg gcggcggagg acgtaggcga tcagcagcag gacgaccagc gctatcacgc
     1321 cgtagccgag ggatctcgca acggcgagga gttcgtccca gtactggccg aagacgtagc
     1381 ccaccagcga gtaggccacg gcccacacga tcaccgcgga gagattatag gggaggaacc
     1441 tgtagtacgt catcctgctt accccggcga acagcggggt catgctgcgc aggcctggcc
     1501 cgaagcgccc gacgaagacc gtcttcccgc cgtgggtctc gaagtagtgt cccaccttgt
     1561 cgaggctttt ctcgttgatc aggaaatgca gaaatcgcat cttgaggatg cggtacatca
     1621 tcggcctccc gccgatgcgc ccgatccagt aaacggaatt gtccgagata aaggcccccg
     1681 cgaacccgct cagcattacc agcggcaacg agagctcccc cccgttggcg aagaggccgc
     1741 cggcgaacag aacgatatcc ccagaagcgg gcagaccgaa gttgtccagg gccgccccga
     1801 agaggatgac gagatacccg tgcgccacga gcaaattcgt cacgaaaacc agaacaccct
     1861 caggcgacct gggtatagat ccaatgaagt ccagaaggct ttgcatgatt cgcagggttt
     1921 ataccacgca acaagcacgc ttcgccctgc gggctccgct cgtcggcacg gcgcttcgcg
     1981 ccttgtcagc acgctcgggc tagcgccctc gctcgtcagc aacgcttcgc ccggcttcgc
     2041 caggctcgcc ttcagccaga tcccgctgcg gcctgcaccc ttccgctcta gagattcgat
     2101 gcccaggaaa ggtgggtagt cgaagccacc gttgcgaatc gagacgacca gccgaaagcg
     2161 aggcgaagcc gaagcgttgc cgacaagcgg agcccgaagg gcggagcgtg cttgttgacg
     2221 tgtgaccgtc tccgggctat gctgcgcgca caagcaagga gcgaaggggc ccaggaagcc
     2281 ggtgtgagtc cggcacggtc ccgccactgt gaccggggag cgaacccgaa gacgtcaccg
     2341 acgcctgagg cggcgggaag gccgggagag caaagagccg gaagtcagga tactggcctt
     2401 tcgccgatgg tcccaaaacg gggcgaggac cccgagaagg aggcacgatg gagaagatcc
     2461 tcgggcggag ctaccgtccc gtactgccgc tgtggagcct gctggttctc gcgttgttgc
     2521 tcctcgcgtt gttcgtattg ctctcggcca gtggggagct gctgctgccg cttctcgggc
     2581 aggcggccgg gccctttgac tacctgcacg agttcgccca cgacgggcga cacctgctcg
     2641 gcgctccctg ccactagatc agacgttggt atcggcctac ctgcgccgcg gcatggccgc
     2701 cgggctgctc gtcggcctcc tgatcggcct cttcgcgttc tttgttgggg agcccgtcct
     2761 ggaccgggct atagccctgg aggaggcgtc cgagggttct catcacgagg acggcgccca
     2821 ccacgaggag atcttctccc gctcgactca gaaggttggc ctcttcttcg ctacggggct
     2881 cttcggcgtg acggtcggtg gcgtcttcgg catcggctac gccttcttcc gcgaccggct
     2941 cgccgcgggt agcgacctcg ggcgcagtgt ctcgctggcg ggcgctatct tcgttgggcc
     3001 gttcttgatc cccttcttga agtacccggc caaccccccg tctgtcggcg atccatccac
     3061 gatcagggag cgtaccgcgg cctacttcgc gctcgtcgcc ctctcgctac tgacgatgct
     3121 cgtcgtctgg ctcaccgcca gaaccctgaa agcgcgcggt ttcggggccc cgagacgccg
     3181 cgtcatggta ggggccgggc tggttctcgt tctagctata cttttcgtgt ccctccccgc
     3241 cgggccctcc gcagaggggt tcccttccgg cctgctctgg gccttcaggt tctcgtcgat
     3301 tggtacccag ctggttttct gggcgggtct ggggctgctc ttcggatggc tgtgcgagcg
     3361 ggcgcgacgg aaggggacag cttgagcggc gccctgctcg ttgcgggaac ccactcggac
     3421 gccggtaaga gcgtggtcgt ggccggcatc tgccgctggc tggcgcgcga gggcatcggg
     3481 gtcgccccgt tcaaggccca gaacatggcg ctcaactcgt tcgtcacgcg ggagggggcg
     3541 gagataggtc gcgcccaggc tgcccaggct gccgccgcag gggtcgagcc agaggctgcc
     3601 atgaaccccg tcctcctcaa gccctccgcc gagaggatca cccaggtcgt tgtgcgcggc
     3661 cgggcgtggg caacggcgag cgccgcgagc tacggcgcga tgaagagaga cctcatgccc
     3721 gtggtgctcg aatcgctcgg ggacctgagg cgccgcttcg acgtcgtgat ctgcgaggga
     3781 gccggcagcc ccgcggagat caaccttcgg aagaacgacc tcgccaacat gggcctggcc
     3841 cgcgccgccc gtctgcccgc cctcctcgtg ggagata
//
LOCUS       DBNGVD010000155         4193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4040332, whole genome shotgun sequence.
ACCESSION   DBNGVD010000155 DBNGVD010000000
VERSION     DBNGVD010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4040332"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16CM_04355"
     CDS             <1..307
                     /locus_tag="AC16CM_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139090.1"
                     /translation="LSLLVMMVLGGGTLSRGDFLPLFRSLSYASAIGLVLWIPFAPIL
                     AIPYGAYVATTAVKETMNVTPARAAAATLIPLGAVVLIILLLLGPSAAGFLVNPPGS"
     gene            complement(304..1518)
                     /locus_tag="AC16CM_04360"
     CDS             complement(304..1518)
                     /locus_tag="AC16CM_04360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR3139091.1"
                     /translation="MSLVRGFEGLQQVIEESFGEKIVALRRDIHREPELGFDTEKTAE
                     KVLAALEGLPLDVRTGIAENGLVATLEGEGDGPTVALRADMDALPILEDTGLSFASET
                     EGKMHACGHDGHTSMLVGAAHALSGMRDRLGGTVKFVFQPAEEGGGGGKVMVDEGVAD
                     GVSSIFALHLWPGLPFGTVATKGGPIMAAADAFEMEVKATGGHGAMPHQGSDAIAIAA
                     QVVTALQTIVSREVDPVEPAVLTVGEIAAGTAFNVIPDRAHLGGTVRTLNADLRRKMP
                     QRIEELARGLAKGMRGDVELDYTFSYPVTVNDGAAADLALDVAEDLFGGGSVLELPNP
                     SMGAEDFSYFLENLPGAFIWLGVGEDVSGLHTPGFAFDEDILPRGSALLTALAIEALQ
                     QAGRFGGDPAGS"
     gene            complement(1676..2311)
                     /locus_tag="AC16CM_04365"
     CDS             complement(1676..2311)
                     /locus_tag="AC16CM_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139092.1"
                     /translation="MRLLSVFFTAAIAMCGFFGMSALASTTAEAQVDAVCPGARVVDN
                     VGPTERDLRIGPFTITGGSFRLTYETSDADQSGLPFFDITVLDQAGKEVGGQVIREET
                     VAREIVRDNPGRFTIEARSEDLKYELTIEDCTGRGNPSSGDPNVPTDPIPRDQYSSDA
                     DPPNEDVIDDTISDEPLPDTGGVSLLGTAVFGSIFVFGAFAVLRPVVRRDP"
     gene            complement(2388..3611)
                     /locus_tag="AC16CM_04370"
     CDS             complement(2388..3611)
                     /locus_tag="AC16CM_04370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3139093.1"
                     /translation="MQASMLSMAVAVALILVWRSPDASGASTATRIDENQRGVPYAAG
                     ELLVTYDKGASRQRIDEVVDQSQATVAEELPRSDAQLLSFPTVKRERGGHVREEKLQR
                     AKVALQKRPGVEHVDFNYARLPQYSPDDTWFLAGEQWGLDNVNAPEAWNKALGVGSRV
                     AVVDTGIAGNHPDLESRIVAQKDLVHNDRVAEDDAAGHGTHVAGTVGAETNNAEGVAA
                     TCPGCKLLVAKSGNVRVLYDSDIVQGIYWSVDNRAKAINLSLGSEGKSRILEHAVDYA
                     WDNGVVVVAAAGNENTDRPSYPAAFRHVISVSATDQADMKARFSNYGTTVDVAAPGVS
                     ILSTVPGDGYGRKSGTSMASPHVAALAGLLASQNLSAPEIRRRIQRTATDLGATGKDD
                     YFGWGLIDANKAVRR"
     gene            complement(3760..>4193)
                     /locus_tag="AC16CM_04375"
     CDS             complement(3760..>4193)
                     /locus_tag="AC16CM_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR3139094.1"
                     /translation="LLGRFEETVDDFVVRRNDGTPGYQLAVVVDDAAQGVGEVVRGAD
                     LVDSTPRQILLSRLLALPEPTYAHVPLVLGPDGQRLAKRHGAVALSDRSEGPEELRSW
                     MARTLGLAEAGEVPSTDDLLNRFDPERLPREPTVWSPSSTA"
BASE COUNT          666 a         1454 c         1296 g          777 t
ORIGIN      
        1 gctctccctg ctcgtcatga tggtgcttgg agggggcacg ctctcgcggg gcgacttcct
       61 acccctgttc cgctccctct cctacgccag cgccataggg ctcgtgctct ggataccctt
      121 cgcccccatc ctcgccatcc cctacggcgc ctacgtggcc accacggccg tcaaggagac
      181 catgaacgtt acgccggccc gggccgccgc cgcaacgctc atccccctgg gggccgtcgt
      241 cctgatcatc ctcctgcttc tgggccccag cgcagccggc tttctggtca acccaccggg
      301 cagctagctt ccggctggat ctccaccaaa ccgtccggcc tgctggagtg cctcgatcgc
      361 tagcgcggtc aacagggcgg agccgcgcgg gaggatgtct tcgtcgaaag cgaaacccgg
      421 cgtgtgcagc cccgagacat cctccccgac gccgagccag atgaaggcac caggcaggtt
      481 ctccagaaag tacgagaaat cctcggcgcc catggagggg ttgggcagtt ccagcacgga
      541 cccgccgccg aagagatctt cggccacgtc aagggcgagg tcggccgcag ccccgtcgtt
      601 gacggtgacg gggtaggaga aagtgtagtc gagctccacg tcgcccctca tgcccttcgc
      661 caacccgcgg gccagctctt ctatgcgctg cggcatcttc cggcggaggt cggcgttcag
      721 ggtacgaacc gttcccccta gatgggcccg atcggggatc acgttgaagg cggttccggc
      781 ggctatctcc cctaccgtca gtaccgctgg ctcgacgggg tccacctccc tggagacgat
      841 ggtctggagc gccgtcacga cctgcgccgc gatggctatg gcgtcggagc cctggtgggg
      901 catagcgccg tgcccccccg ttgccttcac ctccatctcg aaggcgtcgg cggcggccat
      961 gatgggaccg cccttggtcg ccaccgtgcc gaagggcagc cccggccaga ggtgcagcgc
     1021 gaagatggac gagacaccgt cggcgacgcc ctcgtcgacc atgaccttgc cgcctccccc
     1081 gccctcctcg gccggctgga agacgaactt cacggtgcca cccagccgat cccgcattcc
     1141 ggatagcgcg tgggccgcgc cgacgagcat gctggtgtgc ccgtcgtggc cgcaggcgtg
     1201 catcttgccc tccgtctccg aggcgaaaga caggccggtg tcctccagga tggggagtgc
     1261 gtccatgtcc gcccgcagcg ccaccgtcgg cccgtccccc tcaccttcca gcgtggccac
     1321 gaggccgttc tcggcgatcc cggtccgaac gtcgaggggc aggccttcga gggctgcgag
     1381 caccttctcg gcggtcttct ccgtgtcgaa gcccagctcc ggctccctgt ggatgtcgcg
     1441 cctgagcgcg actatcttct ccccgaagct ctcctcgatc acttgctgca acccttcgaa
     1501 cccgcggact aagctcatcc ctagttcctt ccctttaaga cctaccgggc aagcctagtt
     1561 tacagccgac acggggcagc ggtgaattgc gggcgcgcat cctggcacgc acgttcatcc
     1621 tccagtcaag aacagcgggc tgacggctga tagctgaaag ctgaccgctc cggaatcagg
     1681 gatcccggcg taccaccggc ctgaggaccg cgaacgcgcc gaagacgaag atggagccga
     1741 agacagcggt gcctagcagg gacacgccgc ccgtgtcggg caggggctcg tcggagatcg
     1801 tgtcgtcgat gacgtcctcg ttagggggat cggcatcgct actgtactgg tccctgggga
     1861 tgggatccgt cggtacgttc ggatcccccg aggatgggtt tcccctgcct gtgcagtcct
     1921 cgatggtgag ctcgtacttc aggtcttccg accttgcctc gatggtaaag cgaccagggt
     1981 tgtcgcgcac gatctccctc gcgacggttt cttcccggat gacctgtccg ccgacctcct
     2041 tgcccgcttg gtccagcacc gttatgtcga agaacggaag cccgctctga tccgcgtccg
     2101 aggtctcgta ggtcagacga aaacttccgc cggtgatggt gaagggcccg atcctcaaat
     2161 ccctctcggt ggggccgacg ttatctacca ccctggcccc cgggcacact gcgtccacct
     2221 gggcctccgc cgtcgtgctg gcgagcgcgg acatgccaaa gaacccacac atcgcgatcg
     2281 ccgccgtaaa gaacacgctc aacaggcgca tgcgtccccc cataggtctt gtcgtctcat
     2341 tgttgcgtgc gctggtgggc gacgcaagcc tgtgcccgtt cctccgctca gcgcctcacc
     2401 gccttgttcg cgtctatcag cccccagcca aagtagtcgt ccttccccgt ggccccgaga
     2461 tcggtggcgg tcctctgtat gcgcctgcgg atttccggcg ccgaaaggtt ctgggaggcg
     2521 agcagaccgg ccagtgcggc cacgtgcggg gaggccatgc tcgtgcccga ctttctgccg
     2581 taaccatcgc cggggacggt ggacaggatg ctcactccgg gggcggccac gtcgaccgtc
     2641 gttccgtagt ttgagaaacg ggccttcatg tcagcttgat cagtggccga tacggagatc
     2701 acgtgcctga aggccgcggg gtagctgggc ctgtccgtgt tctcgttgcc ggccgcggcc
     2761 acgaccacga ccccgttatc ccaggcgtag tctacggcgt gttcgagtat cctggacttg
     2821 ccttcggatc cgagcgaaag gtttatggcc ttggcacggt tatcgacact ccagtagatt
     2881 ccctgaacta tgtcggagtc gtagaggacc ctgacgtttc cgctcttggc aaccaggagt
     2941 ttgcagcccg ggcacgtcgc ggcaaccccc tcagcattgt tcgtctcggc gccgaccgtt
     3001 ccagccacgt gcgtgccgtg gccagccgcg tcgtcttcgg ctaccctgtc gttgtggacc
     3061 aggtccttct gcgcaactat ccggctctcg aggtctggat ggttcccggc gatgccagta
     3121 tccaccaccg ctaccctcga ccccacgccc agggccttgt tccacgcctc gggggcgttg
     3181 acattgtcca gaccccactg ctcgccggcg aggaaccagg tgtcatcggg agaatattgc
     3241 gggagccggg cgtagttaaa gtcgacgtgc tcgacgccgg ggcgcttctg gagggctact
     3301 ttcgccctct ggagcttctc ttccctgacg tgtcctcccc gttcgcgctt caccgtcggg
     3361 aacgagagaa gctgggcatc cgagcgggga agttcttctg ctacagtagc ctgcgactgg
     3421 tcgactacct cgtcgattcg ctgccgggaa gcccccttat cgtaagtgac cagcagctcg
     3481 cctgccgcgt aggggacgcc tcgctggttc tcatcgattc tcgtagcggt gctagcccca
     3541 ctggcatccg gcgatcgcca gaccagtatc agggcgacgg ccacggccat cgatagcatg
     3601 ctggcttgca agagactcgt tcccctacca gatcgcaccc tccaccctcc cgactctaag
     3661 cacctgataa ccgaagcgcc attatgcctg tgggcgcgaa cgatgccaat atgccacccg
     3721 gacatacgtt gctgcctgaa cagaaaggtt ccagacccct cacgcggtcg aggatggcga
     3781 ccatacggtc ggttcgcgcg gcaggcgttc cgggtcgaaa cgattcagga gatcgtccgt
     3841 ggacggcacc tctccggctt ctgcgaggcc tagcgtgcgc gccatccacg agcgcaactc
     3901 ctcgggccct tcgctccgat ccgagagcgc cacggcgccg tggcgcttgg cgaggcgctg
     3961 accgtcgggg ccgagcacga gcgggacgtg ggcgtaggtc ggttccggca gggcgaggag
     4021 gcgtgagagc aggatctggc gcggcgtcga gtccacgagg tccgcacccc tcacgacctc
     4081 tcctacgccc tgcgcggcat cgtcgaccac gacggcgagc tggtagccgg gcgtgccgtc
     4141 gttgcggcgc acgacgaagt cgtcaaccgt ctcctcgaag cgtcccagca gac
//
LOCUS       DBNGVD010000156         4078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3310998, whole genome shotgun sequence.
ACCESSION   DBNGVD010000156 DBNGVD010000000
VERSION     DBNGVD010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3310998"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="AC16CM_04380"
     CDS             <1..867
                     /locus_tag="AC16CM_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3139095.1"
                     /translation="VREQGMGSSMLGFANLYGDVPGGQAEFLRVPQAQYGPIKVPEGG
                     SEMPDERFVYLSDVLPTSWQAVQYAGIPEHGSVAVFGLGPIGQMSARIAKHQGARVIA
                     IDFVPERLEMARRHGIEVVDTNNVDNVADTLREMTDGRGPDAVIEAVGMEAHGSPATG
                     LAQTITGLLPDSLAQQALNKGGVDRMSAFYQAVDAVRRGGTISAIGLYGGMADPVPML
                     MLFDKQIQLRLGQANVKHWVDEIMPLLVDSSDPLGVDDLATHRLPLEEAPHGYEIFQK
                     KQDGAIKVLLEP"
     gene            complement(1001..1183)
                     /locus_tag="AC16CM_04385"
     CDS             complement(1001..1183)
                     /locus_tag="AC16CM_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139096.1"
                     /translation="MEEYENTFEWAPPSQAWLEAAGWIAEEKRARLRDDPTGEAFARL
                     AFQLCMQQTQKTEPYG"
     gene            1415..2518
                     /locus_tag="AC16CM_04390"
     CDS             1415..2518
                     /locus_tag="AC16CM_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3139097.1"
                     /translation="MTEILRGERDGRRQEGKASKASVVDVDIHEMLTSVRDLAPYLDE
                     PWRSRVDVNDGYKGPPGNPYSFPQPTGVAIQDAVTDDGSPAGSKLELVQEQVLDRYGI
                     ENAILISLFHPTDNQIQPEFATALASAYNDWVLENWLERDERLRSSITVAAQEPRDAA
                     REIDRVGSDPRFVQVILPAVAHHVFGRPFYWPIFEAAQRNDLVVAFHQSGGTRTAVGL
                     PPYYIEWHTAISQAWQSQLISLIAGGVFDEFEELKVAMIESSWTWVPHLMWRFDYNYR
                     SLRREVPWVKRLPSEYIRERVRFSTQPMEYPEDPGDLYRMFEMIGSDEFLMFATDYPH
                     WDFDSPEQALPHTFPRALKEKILSSNARNFYGF"
     gene            2508..2912
                     /locus_tag="AC16CM_04395"
     CDS             2508..2912
                     /locus_tag="AC16CM_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3139098.1"
                     /translation="MASRGALTDAAGSGQKRHVVCRLEDIPPGAVRGYRTGNRRIAVA
                     RLDDGSFRVVADTCPHQAARLSLGSIERMWVSDAPGTLRQSETRNVLLCPWHGFEFDL
                     ETGGDPCVPGRPDLRLKTYRVEVVDGEVAVYL"
     gene            2969..3154
                     /locus_tag="AC16CM_04400"
     CDS             2969..3154
                     /locus_tag="AC16CM_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139099.1"
                     /translation="MRRFSARMDIVWLGLGLLGIGLCVAVLATALAGTTGTVLSALGL
                     PAMLAGVMLLDSARHRR"
     gene            3249..3581
                     /locus_tag="AC16CM_04405"
     CDS             3249..3581
                     /locus_tag="AC16CM_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3139100.1"
                     /translation="MAKRQNVNKMMQQVQQMQQQMQQAQEELANETVTASAGGGAVRA
                     TMTGGMELVSIEIDPEVVDPEDVEMLQDMVLAAVNEAMTSSQELANKKMGGITGGLGD
                     LGLNIPGL"
     gene            3588..>4078
                     /gene="recR"
                     /locus_tag="AC16CM_04410"
     CDS             3588..>4078
                     /gene="recR"
                     /locus_tag="AC16CM_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3139101.1"
                     /translation="MATYARPVERLITELSKLPSIGPRSAQRIAFHVIRGKKEDAIGL
                     AEALQEVKEKIKPCRRCFNLTEAEECEICRDARRDRTVICVVEDPYDIGPIERTNEYR
                     GLYHVLGGALSPLDGIEPEDLRIAELVERVRNEGTTELVVATNPNTTGEATALFIAQE
                     VKDL"
BASE COUNT          794 a         1274 c         1313 g          697 t
ORIGIN      
        1 gtgcgcgagc agggtatggg atctagcatg ctgggcttcg ccaacctgta cggtgacgtg
       61 cccggcgggc aggctgaatt cctgcgcgtt ccgcaagccc agtacggtcc catcaaggtg
      121 cccgagggag gttcggagat gcccgacgag cggttcgtct acctctccga tgtgcttccc
      181 acctcctggc aggcggtcca gtacgccggg ataccggagc atggcagcgt cgcggtcttc
      241 gggcttggcc cgatcggcca gatgagcgcg cgaatagcga agcaccaggg cgcacgcgtg
      301 atcgcgatag atttcgtgcc cgagcggctc gagatggcgc gtcgccacgg catagaggtg
      361 gttgacacca acaatgtcga taacgtggca gacaccctcc gcgagatgac cgacggccgg
      421 ggccccgacg cggtgatcga agcggtcggg atggaggccc acggctcgcc cgccaccggg
      481 ctggcgcaga cgataaccgg cctgctaccc gactccctgg cacagcaagc cctgaacaag
      541 ggcggggtcg accgcatgag cgccttctac caggccgtcg atgccgtgcg caggggcggc
      601 accatctccg ccatcggcct ctacggcggc atggccgacc cggtgccgat gctgatgctg
      661 ttcgacaagc agatacaact ccgcctggga caggcgaacg tgaagcactg ggtcgacgag
      721 attatgccgc tcctggtcga ttcctcggac cccctcggcg tcgacgacct ggccacccac
      781 aggctgccgc tcgaggaggc gccgcacgga tacgagatct tccagaagaa gcaggacggg
      841 gcgatcaaag tcctgctaga gccctgagct agaacatagc aaggagccaa cgcaagaatc
      901 cggagagccc gggtgacgaa ccaccggcgg tagtcagcgg atcgtcaccc gggttccccg
      961 atcttcgtca ccacccggac acaacacgtc tgtggtgttt ctacccgtag ggttcggtct
     1021 tctgggtttg ttgcatgcac agctggaagg cgagcctggc gaaggcttcc cccgtcgggt
     1081 catcgcgaag cctcgctctt ttctcttcgg cgatccagcc cgcagcctcc aaccacgcct
     1141 ggctcggcgg tgcccactcg aaggtgttct catactcctc catcgtccta ttctagcgca
     1201 ggctgcagag cgctcctgcc cgatgtacgg aatgttcggc cgccaccgga aactcgcgtt
     1261 tccccggagg aatggcgatt acgtccctcc gtaaggcccc gtgccagaga tcttgatatc
     1321 atccttcctt gaccatccat aataggtaag ctagcctgct agagtattgt taagtttaat
     1381 aacaaacaaa gttaactaag ggaggttcgt tcgcttgacc gaaatattga ggggcgagag
     1441 ggacggtcgg cgccaggagg gcaaagcgag caaggcttcg gtggtggatg tcgacatcca
     1501 cgagatgctc acctcggtca gggacctcgc gccctacctt gacgagccct ggcgcagccg
     1561 tgtcgatgtc aacgacgggt acaagggccc ccccggcaac ccctactcct tcccccagcc
     1621 caccggcgtg gccattcagg atgccgtaac cgacgatggc agtcctgcag gatccaagct
     1681 ggagctggta caggagcagg ttctcgaccg ctacggcatc gagaacgcga tcctgatcag
     1741 cctcttccac ccgaccgaca accagataca gcccgagttc gccaccgcgc tggcgagcgc
     1801 ctacaacgac tgggtactcg agaactggct tgagcgtgac gagcggctca ggagctctat
     1861 cacggtggcc gcacaggagc caagagacgc agcccgcgag atagaccgcg tgggctccga
     1921 cccccgattc gtgcaggtca tcctgccggc cgtggcccac cacgtcttcg ggcggccctt
     1981 ctactggccc atcttcgagg ctgcccagcg aaacgatctc gtagtcgcct tccaccagag
     2041 tggggggacc aggacggcgg ttggtctgcc cccgtactac atagagtggc acacggccat
     2101 aagccaggcc tggcagagcc agctcataag cctcatcgcg ggcggggtct tcgacgagtt
     2161 cgaggagctc aaggtggcga tgatcgagtc gagctggacg tgggttccgc acctgatgtg
     2221 gcgcttcgac tacaactacc ggagcctgag gcgggaggtg ccctgggtga agcggttgcc
     2281 gagcgagtac ataagggaga gggtcaggtt ctccacgcag cccatggagt acccggagga
     2341 tccgggcgac ctctacagga tgttcgagat gatcggttct gacgagttcc tgatgttcgc
     2401 gacggactac ccgcactggg acttcgactc cccggagcag gcgctgccgc acaccttccc
     2461 gagggccctc aaggagaaga tcctctcctc caacgccagg aatttctatg gcttctaggg
     2521 gcgctctcac ggatgcggcg gggtctggcc agaagaggca cgtcgtctgc cggcttgagg
     2581 atatcccgcc gggagccgtg aggggataca ggacggggaa ccgcaggatc gcggtggccc
     2641 gcctcgatga cggctcgttc cgcgtcgtgg ctgatacctg cccgcaccag gctgcccggc
     2701 tctctttagg ctccatcgag aggatgtggg tctccgacgc ccctggcacg ctccggcagt
     2761 ccgagacccg caacgtgctt ctgtgtccct ggcacggctt cgagttcgac cttgagacgg
     2821 ggggcgaccc gtgtgtgccg ggacgccccg acctcaggct caagacctat cgcgtggagg
     2881 ttgtggacgg cgaggtcgcc gtctacctct agggtagccg tactcccggg atgcgaggga
     2941 gagacggatc tataaaagca gaaatctgat gcggaggttc tcggcgagga tggatatcgt
     3001 ctggttgggt ctggggctgc ttggaatagg cctgtgcgtc gcggtcctgg cgaccgcgct
     3061 ggctggtacg acgggcaccg tgctgtccgc gcttgggtta ccggccatgc tcgccggggt
     3121 aatgctcctg gacagcgccc gccaccgcag gtagccggcg gggcgagtcg gcccggttct
     3181 cacactccct tcccgggcaa aagtcctata atcatttgat tcgtggacag agagatccgg
     3241 aggttcgggt ggcgaagcgg cagaacgtaa acaagatgat gcagcaggtc cagcagatgc
     3301 agcagcagat gcagcaggct caggaggagc tcgccaacga gaccgtcacg gcgagcgccg
     3361 gcggcggtgc cgtcagggcg accatgaccg gcggaatgga actcgtctcg atagagatag
     3421 accccgaggt cgtggacccc gaggacgtcg agatgctgca ggacatggtg ctcgccgccg
     3481 tcaacgaggc aatgacgagc tcgcaggaac ttgccaacaa gaagatgggt ggcatcaccg
     3541 gcggcctcgg cgacctcggc ctgaacatac ccggtctcta gagctctttg gcgacttacg
     3601 caaggcctgt agagaggctc ataaccgagc tctccaagct gccctccatc ggaccgagga
     3661 gcgcgcagcg catcgccttc cacgttatca ggggcaagaa agaagacgcc atcgggctag
     3721 ccgaggcgct gcaggaggtc aaggagaaga tcaaaccctg caggcgttgc ttcaacctga
     3781 ccgaggctga ggagtgtgag atctgccgcg acgcccgcag ggaccggacg gtgatctgcg
     3841 tggtcgaaga cccctacgat attggtccga tcgagaggac caacgagtac cgcggcctct
     3901 accacgttct gggcggcgcc ctctctcccc tggacggcat cgaaccagaa gacctccgca
     3961 tcgcggagct cgtggaacgc gtccgcaacg agggtaccac cgagctcgtc gtcgcgacca
     4021 acccgaacac gaccggtgag gcgaccgcgc ttttcatcgc gcaagaggtc aaggacct
//
LOCUS       DBNGVD010000157         3129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4587242, whole genome shotgun sequence.
ACCESSION   DBNGVD010000157 DBNGVD010000000
VERSION     DBNGVD010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4587242"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..576
                     /locus_tag="AC16CM_04415"
     CDS             94..576
                     /locus_tag="AC16CM_04415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HIR3139102.1"
                     /translation="MTLVQVLLGITVLSTGLFTGLLMTVLFFFERALKELSGTEFAVV
                     MQRFLKITRTHPLNYAMVLISGFAPIAVLVMVRETPGSPAFVLTAAGLLAFWCGPILT
                     SRFIAQPVYGVFMSWETDAPPKDWRKARDRYFHANLVRGPSSAIAFVCFVVAAMIVPL
                     "
     gene            784..1284
                     /locus_tag="AC16CM_04420"
     CDS             784..1284
                     /locus_tag="AC16CM_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3139103.1"
                     /translation="MKELHSEIEIAASAQRVWRILTDFASYPRWNPFIRRISGEPTTG
                     ERLEVRLEPPDSRGITLRPTVLRAEPTCELRWLGHLLVPGLFDGEHRLVIQPIAENRV
                     RFVQRETFKGLLVPLLARSLDDSTRRGFEEMNRALKVRAEALPADTDPRTSGQSVRRS
                     REDQER"
     gene            1326..1730
                     /locus_tag="AC16CM_04425"
     CDS             1326..1730
                     /locus_tag="AC16CM_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139104.1"
                     /translation="MEQRNIGTTESRRRPADGGYAGRDGKREYEVAGSEESGHRREED
                     LTTYEIRVKGHLDSRWSEWFDGLEITNLENGEAALSGDIVDQAALHGLLTRVRDLGLP
                     LVGVRRLDPQGEQDSARTPTSGTGRGGGPPLL"
     gene            1815..>3129
                     /locus_tag="AC16CM_04430"
     CDS             1815..>3129
                     /locus_tag="AC16CM_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3139105.1"
                     /translation="MEDTNGKYRSAPTEVPYASQAPDEVEVAVVGAGPAGLTVAAMLA
                     GYGVRVAVLDGASGPAEHSRAAVVHARTLEALDPLGVVDEALREGVIVGHFGVRDRDR
                     RLLAVDFDGLPTPHPYTLMLPQDDTEGLLRGALRRRGGEIAWEHEVVGVRQDAGGVEL
                     KIRAPRGEEWVRAKHVVACDGAHSSVRDAVEIPFEGETYPQSFVLADVRMGWALPDDE
                     VQLFFSPEGLVVVAPLPGGSHRLVATVDEAPAEPSLNDVQELLDTRGPRTSRTRVEEV
                     VWSSRFRVAHKVAARFREGGVFLCGDAAHVHSPAGGQGMNTGIQDAANLAWKLALVLR
                     GHVDEGAAGALLDSYEAERRQVALGVVSTAHRLTRLATVRSPVARRLRNALLAVAGLT
                     GPLPRRLAANLAEVDIVYRGGWSADGSNAVGRWIPEGGMAAAENQD"
BASE COUNT          556 a          973 c         1096 g          504 t
ORIGIN      
        1 atcggggaga tcaacctgga ggtggtttaa ggctgctttg gtcattctgt tgtttatgag
       61 gctgagtaaa caatcggaag aaaggagaac gccatgaccc tggttcaagt ccttctcggc
      121 atcaccgtcc tcagtactgg gctcttcacg gggctgctga tgaccgtgct gttcttcttc
      181 gaaagggcac ttaaggagct gtccggcacc gagttcgccg ttgtaatgca gcgcttcctg
      241 aagataacgc gcacgcaccc cttgaactac gccatggtgc tcatctctgg ctttgcaccc
      301 atcgcggtgc tggtgatggt gcgcgaaacc ccgggaagtc ccgcgttcgt gttgaccgcg
      361 gcgggcctgc tcgcgttctg gtgcggtccc atcctcacct cgcgctttat cgcccagcca
      421 gtctatggcg tctttatgag ttgggagacc gatgcgcctc ccaaggactg gcggaaagct
      481 cgcgaccggt acttccacgc caacctggtc cgcgggccga gctcggcgat agccttcgtc
      541 tgcttcgtgg tcgcggccat gatcgtgcca ctgtaggcgc gaaaggggag attgatatga
      601 gaggaaggaa gccatagaag gtcctttccg cggacaacag gcctgggctc gagaagcttg
      661 ccccgggagg aacccggata cgaagccccc ggctcctccc gtgctttgtt cgccggatct
      721 catggaaggt gaacggatgt agatccgtgg gatagacgcc gggcgtgaca ggaggtagtg
      781 ccgatgaaag agctgcattc cgagatcgag atcgccgcca gcgcacagcg cgtgtggcgt
      841 atcctcaccg acttcgcttc ctatccccgg tggaacccgt ttatccgccg gatcagcggt
      901 gagcccacta ccggcgagcg cctcgaagtc cgcctcgagc ctccagatag caggggcata
      961 accctccggc ccacggttct cagagccgaa cccacctgcg aattgcggtg gctgggccac
     1021 ctgttggtgc cgggcctgtt cgacggcgaa caccgcctgg tcatccagcc aattgcggag
     1081 aaccgcgttc gtttcgtcca gagagaaacg ttcaagggcc tgctcgtccc cctgctcgcc
     1141 cgcagcctgg atgacagcac gcggcgcggg ttcgaggaga tgaaccgagc cctcaaagtg
     1201 cgggccgagg cgcttcctgc ggataccgac ccccgaactt ccgggcagtc cgtcaggcgg
     1261 tccagagagg atcaggaaag gtgacccgat ctagcaacgg ctcggaccct gggaaggagc
     1321 agaagatgga gcaacgaaat atcggaacga ccgaaagccg ccgtcgtccg gcagacggcg
     1381 gctacgccgg gcgggacggt aagcgcgagt acgaagtagc cggatcagaa gagtcgggtc
     1441 atcgacggga ggaggatttg actacctacg agatccgggt gaagggacac ctcgattcgc
     1501 gctggtcgga gtggttcgac gggttggaga tcaccaacct ggaaaatgga gaggccgcgc
     1561 tatccgggga catcgtggac caggccgcgc tgcacggatt gttgaccagg gtacgtgacc
     1621 tgggactgcc tctggtaggc gtgagaaggc tcgatccgca aggcgaacaa gattccgccc
     1681 gaacaccgac gtccggaacg ggtagaggcg gcggcccacc gcttctatga accggttgcc
     1741 cgccgatgag gggcaaacgg ccacgtacgg gcgatggcga gcaaaaggtc acgggcagag
     1801 gaaggacgac gaatatggaa gacacgaacg gaaagtatcg ctcggcgccc acggaggttc
     1861 cctacgcaag ccaggcgcct gacgaggtag aggtcgcggt ggtgggggcc ggcccggccg
     1921 ggctcacggt ggcggcgatg ctggcggggt acggggtacg cgtggccgtg ctcgacgggg
     1981 cctccggtcc tgcggaacac tcccgcgcgg ccgtcgtcca cgcgaggacg ctggaggccc
     2041 tcgacccctt gggcgtcgtc gacgaggcgc tgcgggaggg cgttatcgtg gggcatttcg
     2101 gcgtgcgcga cagggaccgc cggctcctcg ccgtggactt cgacgggctg ccgacgcccc
     2161 atccctacac gctcatgctc ccgcaggacg acaccgaagg cctcctacgg ggggcgctgc
     2221 gccggcgggg cggggagatc gcgtgggaac acgaggtcgt cggggtgcgc caggacgcgg
     2281 gaggcgtcga gctcaagatc cgtgccccgc ggggagaaga gtgggtgagg gccaagcatg
     2341 tggtcgcctg cgacggcgcg cacagctcgg tgcgggacgc ggtggagata cccttcgagg
     2401 gagagaccta cccccagtcg ttcgtgctcg cggacgtgcg catggggtgg gctctgcccg
     2461 atgacgaggt gcagctcttt ttctcccccg agggcctcgt cgtggtggcc ccgttaccgg
     2521 gcgggagcca ccgcctcgtc gccacggtcg acgaggcgcc cgctgaaccg tccctgaacg
     2581 acgtccagga gcttctggac acccgcggac cgcgcacctc gcgtacccgc gtcgaggagg
     2641 tcgtctggtc atcgcgcttc cgcgtagccc acaaggtcgc cgcccggttc cgcgagggag
     2701 gcgtcttcct ctgcggcgac gcggcgcacg tccacagccc cgccggcggg cagggcatga
     2761 acaccggcat ccaggacgcg gccaacctcg cctggaagct ggcgctcgtg ctccgcggac
     2821 acgtcgacga gggggccgcc ggagcgctgc tcgactccta cgaggcggag cgcaggcagg
     2881 tcgccctcgg tgtcgtctcc accgcgcacc ggctgaccag gctggccacg gtgcgctcgc
     2941 cggtggcccg gcgcctcaga aacgccctcc tagcggtggc cggcctaacc ggaccgttgc
     3001 cgaggcgact cgcggcgaac ctggcggagg tagacatcgt ctaccgcggc gggtggagcg
     3061 cggacggctc gaatgccgtg gggcgttgga taccggaagg cgggatggcg gcagcggaga
     3121 atcaggaca
//
LOCUS       DBNGVD010000158        11447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3676658, whole genome shotgun sequence.
ACCESSION   DBNGVD010000158 DBNGVD010000000
VERSION     DBNGVD010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3676658"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..849)
                     /locus_tag="AC16CM_04435"
     CDS             complement(118..849)
                     /locus_tag="AC16CM_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3139106.1"
                     /translation="MEPLSRNSAGTLYHQLLRILRGRIESGEIGVGDRLPSEAELVSD
                     FGVSRTTARRALDELRREGLVRREPGRGTFLVSPRLRSNTAYLHSFTEEIKRWGYTPG
                     ARLISREEGEASEQVASRLEIESGEEVLFVRRLRLADELPIFVCDSYLPVGRFPALKD
                     ADYGTVSLSKLFQERIGRGIQNSRQWIGAAAATSDVAALLEIPPGIPVLHVRRISYVA
                     GGSPIELVEAYFHPERYQHYNELHV"
     gene            1129..2478
                     /locus_tag="AC16CM_04440"
     CDS             1129..2478
                     /locus_tag="AC16CM_04440"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3139107.1"
                     /translation="MFEFPIKEFFKQPRSLLGAGAYENAGPEAAAMGLKHVLFVTSGL
                     SGTGIIDECKTNFENAGVSVTVYDKVESNPKDYNVMDAYEAYSEGECDGFVSIGGGSS
                     HDTTKGARIVAAHDGRNVNEFQELNASETLDNPAHIAINTTVGTGSETTPAYVITDLS
                     SPEAPHKWVGFDRAAITTLAINDPVLCMTQPPEYVAYTGFDTLAHASECYVGRVQHLS
                     TTPIALAAIRLTQENLREATYNPKNYKAMEAMVWAQYTAAQAFSTGLLGILHSLSHAV
                     CAYYDIHHGLNNGVGLPRVWTYNQPAAPEKYADIAEAMGVDTKGMNVPQAADAAIEAV
                     IRLVKDCGCPESWSQVGEYPKTRMGKGYYEGKTNAAIQSDDDELQKMAEHMMGDLCTP
                     GNPRDLTVEGAREVLRDCMYDGMAVKTGNGYRQNVWGVAPEMGRRTQQTPTERPGPQ"
     gene            2586..3581
                     /locus_tag="AC16CM_04445"
     CDS             2586..3581
                     /locus_tag="AC16CM_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3139108.1"
                     /translation="MFESIEEVREKLAGQEYVCDRRLATVVYLAVKLDKPVLVEGPAG
                     VGKTELAKSLAAATDRTLIRLQCYEGLDEAKALYEWEYGKQLLYTQVLREKIGEVLGP
                     TEGLREAAATLREQEDVFFSENFLVERPILQSITSEIPPVLLLDEIDRADEEFEAFLL
                     EFLSDYQVSIPEIGTITSEHAPVVVLTSNRTRELSEALKRRCLHLQLDYPSPEVELEI
                     VRLKATGVGEALSRDLVQTIQELRTLDLRKAPSIGETLDWARSLVVLGAPHLDKDLIE
                     ETLSVIVKYDRDAEKVLAHVGAGPGRKDLGQKEPTGENPHEAMYEMHRSVHKHTH"
     gene            3709..5232
                     /locus_tag="AC16CM_04450"
     CDS             3709..5232
                     /locus_tag="AC16CM_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3139109.1"
                     /translation="MDTVVNDFVQVLRRHQVRVSPAESLDALHALGQTGLGERGVVRD
                     TLRTTLVKNSEDIETFDRLFDLYFGLRPTAEKRTARLDAHGHDHDHGEPTSMEFGEEA
                     EGEAPGSEEHSHEENENTEFRRHFDEDKMRPSEDIHGEADKMRLSMFSQELMLNRKPG
                     ALDQALQRITHQLKLRRARGMFSPGDLVTHGEGGQELPLDVSAADFQGLIDHLHDLDV
                     DESLIRQLEEQSEEIMRGLPQLIEQMVERQKQLEKKDDFALMRRSLRKVFDYSPAEQR
                     QTEAAIRRIARQIHGAKTRRLRQDRTGHISVPHTLRRNVRYEGIPFNPVLRRHHEKRP
                     RVVMLCDVSMSTRNMSRFWLHMVHQMQSLFSKVRTFVFVADVAEVTQYFEESSMDRAV
                     DQVFSGRVIDADVNSDFGRAAGQFRNEYLPSLNHRTTLVILGDGRNNGKAPNTEALEE
                     ISHHVRQTIWITPEPKWGWSLGSCDMPAYEPICDRVEVVRSVDQLAGVAENLVRARA"
     gene            5229..5759
                     /locus_tag="AC16CM_04455"
     CDS             5229..5759
                     /locus_tag="AC16CM_04455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3139110.1"
                     /translation="MTERVPFSNTEPYRFCPADGTGLQAKGGESSGVSCPTCGRSWYR
                     SSSPAVGAAIVQGGHVLVTVRGIEPEKGKLDLPGGFVEIGEHPRDALVREAQEELGVE
                     VEVEERPMLLAVHTYGEDGEYVLAIGFRTRIISGEPDPADDVADIRWATKDEVDTLDF
                     AWEHDRRIARRALEDA"
     gene            5822..6580
                     /locus_tag="AC16CM_04460"
     CDS             5822..6580
                     /locus_tag="AC16CM_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HIR3139111.1"
                     /translation="MSAIDGWLEGLLHGEVGVGVILLVSLLLGLRHASDPDHLAAVTT
                     LIASGEDRHRVRKAGTMGLLWGLGHGTTLVLIGLPLVLLNQYLPEIVGKIAEVAIGCI
                     IVLLALRLLVRWRRGLYHVHVHTHDDGEAHRHVHSHVEDESHGHAHRLPKRTPLSSYG
                     VGLVHGIGGSGGLTLLLISTISDKAQATGALFLFAAGTAASMVLLSTAFGLVIAGGPL
                     ARNFERVAPVLGVLSMAFGAWYALGALGIVVYPF"
     gene            6700..7458
                     /locus_tag="AC16CM_04465"
     CDS             6700..7458
                     /locus_tag="AC16CM_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3139112.1"
                     /translation="MGRSPLLAADGKALIVAMDHARAYGAIEGLEDPGAVIEASVEGG
                     ADAVMTTFGVAKRYHDQLAGRIPTVIRLDGGPSLFREDWLAYTEWSLLHSVDDALLLG
                     ADAVVLMAFVGIPVELETYRIIARVAGECLRANLPLVVEALPCPSERIPDPRATEAMA
                     SAARIGFEHGADLVKSYFTEDFEKVTENCPVPVLVAGGPKMDTREETLQVVRDATRAG
                     AAGIVFGRNIWQSEDTKGMIEALSHIIHEDQNTP"
     gene            complement(7503..8975)
                     /locus_tag="AC16CM_04470"
     CDS             complement(7503..8975)
                     /locus_tag="AC16CM_04470"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3139113.1"
                     /translation="MRRVVSVLASATLAVGLLFSGAYSGISVDLRKAAAQTVPARPNI
                     VFILTDDMRKDDLSYMPQTRALLKGEGMSFENAFVSHALCAPSRATIMRGQYAHNAGV
                     WSNSATDSPSTSTGGWQAYQARGNEADNVATRLDDAGYRTALFGKYMNRYAGTTYVPP
                     GWDRWFAIATSGEPQYFGYDVVSEDQTISHYGTNEADYTTDVLSNEAGEFMRNSALQG
                     QPFFAYVAPIAPHSPATPAPRDEHAYDGVTGPRFSSFDEEIVDDKPSWIRQLPRLTAD
                     RVTEIDNRHERRVESLQAVDDLVQNVVLTLDDAGELDNTYIFFTSDNGFHYGEHRIPM
                     QKWRPYEEDVNVPLLVRGPDVAPGSTTYKMALNTDYLPTFTRLAGAQTPAYVDGRSLR
                     PVLRNTVTSWRSAVLLEAAAEYSPPYRGVRTVNAGGVPKRKYVEYSGGEKELYELGSD
                     PRELANRYSSSYPAATALAARLQKLKGCARDTCFTFENGP"
     gene            complement(9163..10149)
                     /locus_tag="AC16CM_04475"
     CDS             complement(9163..10149)
                     /locus_tag="AC16CM_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HIR3139114.1"
                     /translation="MITDSAIYVDGKRTGTPDPEGTAPAAVERGGFAWVELVEPDDEE
                     LASVAGEFGLPEAAVRDVVKAHHRPRADRYGEALLVVLRTARYLEEEERVEFGGIHVF
                     LGRDFIVTIDHDRASSLEGVRRELEGHAGFLGKGTGAVLWAVVDRVVNGYDPVIDGLE
                     NDVEEIEEQVFGGEDVDVSRRIYELSREVLEFRRAAQPLSGVLERLFAQVHGSDRELL
                     RRMRGVQDRALRITERIDGFRELLQNILSVNLTIVSVRQNATMQRQNVQVQKISAWAA
                     IIAAPTLISGIYGMNFRYMPELNSVLGYPYALAIMFSISSLLYVGFKRAGWL"
     gene            10274..>11447
                     /locus_tag="AC16CM_04480"
     CDS             10274..>11447
                     /locus_tag="AC16CM_04480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3139115.1"
                     /translation="MSTEPILIGADVGTTNIKVVAFDRSGRALKSASSPTPTRYPRPG
                     RANYDPEELWASFTATLRRVTGGLESPGRVASIAVASVGEAAVPLDRDGEPTSDIIAW
                     FDGRARPQAQRLERTVGQERLFGLTGLSLQPIFGLCKLLWLKENEPDAYSRTAKWLNV
                     ADYMAFRLCGVAATDLSLASRTLALDLQGRRWAEGLLEEVGISPEVFATLRESGSDLG
                     PVTPAAARATGLPEGARVAAGGHDHVCGALALGVTERGTMLNSLGTAEAIFLPLGRPL
                     EDPKVGDQGYTQGAHVAGQYYVFGGQYTSGASVEWMRKILDGADYETLISEAEEVPPG
                     SLGAFFLPHLRLANPPYDDPAARGAFVGLSADVGRGALFRAMLEGLAYDSRNSLEPL"
BASE COUNT         2131 a         3698 c         3742 g         1876 t
ORIGIN      
        1 ctgcgcggcg ataccctcta tgcgaaaggt gcaggccgca gcgggatctg gccgaaagcg
       61 agccctgaaa gggcgagacg tgctgacaag cggaggcccg tagggccgga gcgtgctcta
      121 cacgtgaagt tcgttgtagt gctggtagcg ttcggggtgg aagtacgcct ccaccagctc
      181 gatgggagag ccacccgcga cgtagctgat gcggcgtacg tgcagcacag ggattcctgg
      241 cggtatctcg aggagggcag ccacgtccga cgtcgcggcc gccgcgccga tccactgcct
      301 cgagttctga atcccgcgtc cgatcctctc ctgaaagagc ttgctcaggg agacggtccc
      361 gtagtcggcg tccttgaggg ccgggaaccg tccgacgggg aggtacgagt cgcagacgaa
      421 gatgggaagc tcgtcggcga gccgcagacg cctcacgaac agcacctctt cgccgctctc
      481 gatctccagc ctcgaggcga cctgttcgct cgcctcgccc tcctcgcgag agatcaacct
      541 ggcccctggc gtgtatcccc agcgtttgat ctcttccgtg aagctgtgca ggtacgccgt
      601 gttggagcgc agccgcgggc taaccagaaa cgttccacgc cccggctccc gccgcacgag
      661 cccctcccgc ctgagctcgt ccagcgcccg ccgcgccgtc gtccggctta ccccgaagtc
      721 tgagaccagc tcggcctcgc tcggcaacct gtccccaacc ccgatctcac cagactcgat
      781 ccgaccccgc aagatccgca gaagctggtg gtagagtgtt cccgcgctgt tccgtgacaa
      841 aggctccata acagcatttg tagtatcaca ccattgttgc gtcaagacgt atcggagagc
      901 gccagcgata caggccgttc tataaagcca gtctggcaac agatctccgt gttcctcgaa
      961 aggatctgtt gcacggggtg aaggacgact gttacattgt aataacagtc ttcctgaaag
     1021 gggtgatcgg gagaggcaca agcgtttgag ggggatctat ccggggctcc cagcccaggt
     1081 ggcttgcgtg gatcgagagg aaaggagagg tagttggaca cctcacggat gttcgagttt
     1141 cccataaaag aattcttcaa gcagccgcgg tcgcttctcg gggctggggc ctacgagaac
     1201 gcgggtccag aggcagcggc gatgggtctc aagcatgtgt tgttcgtaac ctcggggttg
     1261 agcgggaccg ggatcatcga cgagtgcaag acaaacttcg agaacgccgg cgtctcggtt
     1321 acggtctatg acaaggtgga gtccaacccc aaggactaca acgtcatgga tgcctacgag
     1381 gcgtactccg agggggagtg tgacgggttc gtctccatcg gcggcggatc ttcgcacgac
     1441 accacaaagg gtgcccgcat cgtggcggcg cacgacggca gaaacgtcaa cgagttccag
     1501 gagttgaacg cttcagagac gctagacaac ccggcgcaca tcgcgatcaa caccaccgtg
     1561 gggacgggct cggagacgac gccggcctac gtcatcacgg acctgtcttc gcccgaggcg
     1621 ccgcacaagt gggtcggttt cgaccgggcg gccatcacga cgctagctat caacgacccc
     1681 gtactgtgca tgacgcagcc gcccgagtac gtggcgtaca caggtttcga cacgcttgca
     1741 cacgcctccg agtgttacgt cgggcgcgtg cagcaccttt cgacgacccc gatagcgctc
     1801 gcggcgatcc ggctgaccca ggagaacttg cgcgaggcga cctacaaccc gaagaactac
     1861 aaggcgatgg aggctatggt ctgggcgcag tacacggcgg cccaggcgtt ctcgaccggt
     1921 ttgctcggca tcctgcactc cctctcgcac gcggtctgcg cctactacga catccaccac
     1981 ggcctgaaca acggcgtcgg ccttccgagg gtgtggacgt acaaccagcc ggcggctccg
     2041 gagaagtacg ccgacatcgc cgaggccatg ggcgtggaca ccaagggcat gaacgttcca
     2101 caggcggctg acgcggccat cgaggccgtc atccggttag tcaaggactg cggctgcccg
     2161 gagagctgga gccaggtcgg ggagtatccc aagaccagga tgggcaaggg ttactacgag
     2221 ggcaagacaa acgcggccat ccagtccgac gacgacgagc tacagaagat ggccgagcac
     2281 atgatgggcg acctgtgcac gcccggcaac ccgcgcgacc tcacggtcga aggcgccagg
     2341 gaagtcctgc gcgactgcat gtacgacggt atggccgtca agacgggcaa cggctacagg
     2401 cagaacgtct ggggggtggc tccggagatg ggccgcagga cgcagcagac ccctaccgaa
     2461 cgtccaggac cccagtagtc aggcggttct cgacgggcga aacgaacagc gggagcgggc
     2521 tcgagcccgc tcccgtcatt tgaaacggta tatttgggga cctgatcttc acgaaaagga
     2581 gcattttgtt cgagagcatc gaagaggtca gggagaagct cgccgggcag gagtacgtct
     2641 gcgacaggcg tctggcgacc gtggtgtacc tggccgtaaa gctggataag cccgtccttg
     2701 tagagggtcc tgcgggcgtg ggcaagaccg agcttgccaa gtcgctcgcg gcggccacgg
     2761 acaggacgct aatccgcctg cagtgttacg aggggctgga tgaggcaaag gccctctacg
     2821 agtgggagta cggcaagcag ctcctctaca cgcaggtgct gagggagaag atcggggagg
     2881 tcctcggtcc gacggagggc ctgcgcgagg ccgcggcgac cctgcgcgag caggaggacg
     2941 tcttcttctc cgagaacttc ctggtcgagc ggcccatctt gcagtccata acctcggaga
     3001 taccgccggt gctcctgctc gacgagatcg accgcgccga cgaggagttc gaggcgttcc
     3061 tgctcgagtt ccttagcgat taccaggtct ccatcccgga gatcgggacc atcacgtccg
     3121 agcacgcccc ggtcgttgtc ctgacctcga accgcaccag ggagcttagc gaggcgctca
     3181 agcgccgctg cctgcacctc cagctcgact acccgagccc cgaggtggag ctggagatag
     3241 tccgcctcaa ggcaaccggc gtcggcgagg cgctgtcgag ggacctcgtg cagaccatcc
     3301 aggagttgag gaccctcgac ctgcgcaagg cgcccagcat cggggagacg ctcgactggg
     3361 cgcgctcgct tgtggtgctc ggcgccccgc acctggacaa ggacctcatc gaggagaccc
     3421 tgagcgtcat cgtcaagtac gaccgggacg ccgagaaggt gctcgcgcac gtcggcgccg
     3481 gccccgggcg caaggacctc ggccagaagg agccaaccgg cgagaacccg cacgaggcga
     3541 tgtacgagat gcaccgaagc gtccacaagc acacgcatta gagcgggccc ccgtgagcag
     3601 tgaagcggag gtggggaaaa gcttcaggtt tcaggcaaca ggtatcaggt atatttcctg
     3661 aagcctgtaa cctgtggcct tcttccaaaa gagtctggaa agtggtttat ggataccgta
     3721 gtcaacgatt tcgtgcaggt cttgaggagg caccaggtca gggtctctcc ggcggagagc
     3781 ctggacgcgc tgcacgcgct ggggcagact gggctcggcg agcgcggggt ggtacgggac
     3841 acgctgcgga ccacgctcgt caagaattcg gaggacatag agaccttcga tcggcttttc
     3901 gacctgtatt tcggcctgcg gcctacggcg gagaagcgga cggcgaggct ggacgcgcac
     3961 ggccatgatc acgaccacgg cgagccgact agcatggagt ttggggaaga ggccgagggg
     4021 gaggcaccgg gctcggagga gcacagccac gaggagaacg agaacaccga gttccgccgc
     4081 cacttcgacg aggacaagat gcgcccttcg gaagacatcc acggcgaggc ggacaagatg
     4141 cggctctcca tgttctcgca ggagttgatg ctcaatcgca agcctggggc cctggatcag
     4201 gcgctgcagc gcataacgca ccagctcaag ctccgccggg cccgcggcat gttcagcccc
     4261 ggcgacctcg tcacccacgg agaaggcggc caggagttgc cgctcgacgt ctccgccgcg
     4321 gattttcagg ggctcatcga ccacctgcac gacctcgacg tcgacgagtc gctcatccgc
     4381 cagctcgagg agcagagcga ggagataatg cgcggcctcc cccagctcat cgagcagatg
     4441 gtcgaacgcc agaagcaact cgaaaagaag gacgacttcg ccctcatgcg gcgctccctg
     4501 cgcaaggtct tcgactactc ccccgccgag cagcggcaga ccgaggcggc catccgacgc
     4561 atagcgcgcc agatccacgg cgccaagacc cgtcgtctga ggcaggaccg cacggggcac
     4621 ataagcgtcc cccacaccct ccgccgcaac gtccgctacg aaggtatccc cttcaacccc
     4681 gtactgcgca ggcatcacga gaagcgtccg cgcgtggtga tgctgtgtga cgtcagcatg
     4741 agcacgcgta acatgagccg gttctggctg cacatggtgc accagatgca gagcctgttc
     4801 tcgaaggtca ggaccttcgt gttcgtggcc gacgtggccg aggtcacgca gtacttcgag
     4861 gagagcagca tggacagggc cgtggaccag gtcttctcgg ggcgggtcat cgacgccgac
     4921 gtgaacagcg acttcggcag ggccgccggg cagttcagga acgagtatct gccctcgctc
     4981 aaccaccgca ccacgctcgt gatcctgggc gacgggcgca acaacggcaa ggcacccaac
     5041 acagaggcgc tggaggagat ctcacaccac gtcaggcaga cgatctggat caccccggaa
     5101 ccgaagtggg gctggtctct aggttcctgc gacatgcccg cctacgaacc catctgcgac
     5161 cgcgtcgagg tggtaaggag cgtggaccag ctagcgggcg tcgccgaaaa cctcgtgagg
     5221 gcccgcgcat gaccgaacgg gtgcccttct ccaacacgga gccctaccgc ttctgcccgg
     5281 cggacggaac gggactgcaa gcgaagggcg gtgagagcag cggcgtatcg tgccccacgt
     5341 gcgggcggtc gtggtacaga agctcgtccc ccgccgtggg ggccgcgatc gtgcagggag
     5401 gacacgtcct cgtgacggtg cggggcatcg agcccgagaa ggggaagctg gatctccccg
     5461 gcggcttcgt ggagatcggc gagcacccga gggacgccct agtgcgggag gcgcaggaag
     5521 agctaggcgt ggaggtcgag gtcgaggaac gcccgatgct gctcgccgtc cacacctacg
     5581 gcgaggatgg agagtacgtg ctcgctatag gtttccgcac gcgcatcatc tccggcgaac
     5641 cagacccggc cgacgacgtg gctgacatca ggtgggccac gaaagacgag gtcgacaccc
     5701 tcgacttcgc ctgggagcac gaccggagaa tagcccggcg ggcactggag gacgcataga
     5761 tgtcgcgcgg cggtatcagg aggaccgttt gctgggattg tcggtacgat gaagggggcg
     5821 tatgtccgcg atagacggct ggctggaagg actcttgcac ggggaggtcg gggttggcgt
     5881 catactgctc gtcagcctgc tgctcggctt gaggcacgct tccgaccccg accacctcgc
     5941 cgccgttacc accctgatcg cctccgggga ggaccggcac agggtgcgca aggcgggtac
     6001 catgggcctg ctctggggac tcggccatgg gaccacgctt gtgcttatcg gcctgccgct
     6061 cgtcctcctg aaccagtacc tgcccgagat cgtgggcaag atagccgagg tcgccatcgg
     6121 gtgcatcatc gtgctgctcg cgctgcggct gctcgtccgg tggcggcggg gcctctacca
     6181 cgtacacgtc cacacccacg acgatggcga agcccaccgc cacgtccact cccacgtaga
     6241 ggacgagtcg cacggacacg cccaccgcct cccgaagcgt accccgctct cctcttacgg
     6301 cgtcgggctg gtgcacggca tcggtggctc cggggggctt acgctgctcc tgatctccac
     6361 catctcagac aaagcccagg cgacgggggc gctctttctc ttcgcagcgg gaaccgccgc
     6421 ctctatggtg ctcctctcca cggctttcgg cctcgtcata gccggcggcc ccctggcccg
     6481 taacttcgag cgcgtcgccc ccgtgctcgg cgtcctcagc atggccttcg gagcctggta
     6541 cgccctcggc gcgctcggaa tagtcgttta tccgttctag cgaatcgcaa gcgaacagca
     6601 agccggcaac gttcgtcagg caatcttcct gtagcctgta gccttctcct gtagagtggc
     6661 tcttgcttta cgaaaacctt ccagggactg gggatggaga tgggaagatc accgttgctt
     6721 gctgctgacg ggaaggcgtt gatcgtggcg atggatcatg cgcgggctta cggcgcgatc
     6781 gaggggttgg aggaccctgg ggcggtgatc gaggcctccg tggagggcgg ggcagacgcc
     6841 gtcatgacga ccttcggcgt ggcgaaacgc taccacgacc agcttgccgg ccgcataccc
     6901 accgtgatcc gtctcgacgg cggcccgagc ctgttccgcg aggactggct cgcctacacg
     6961 gagtggagcc tcctgcattc cgtcgatgac gcgctcctgc ttggggccga cgccgtcgtg
     7021 ctgatggctt tcgtgggcat ccccgtcgaa ctggagacct accgcatcat cgccagggtg
     7081 gccggcgagt gcctgcgcgc caacctgccg ctcgtggtcg aggcgctacc gtgcccgagc
     7141 gagcgcatcc ccgacccgag ggccaccgag gccatggcct cggccgcccg catcggcttc
     7201 gagcacggcg ccgacctggt caagtcgtac ttcacggagg acttcgagaa ggtgaccgag
     7261 aactgccccg tgcctgtcct ggtcgccggt ggaccgaaga tggacacaag ggaagagacg
     7321 ctccaggtcg tccgggacgc aaccagggcc ggcgcagccg gcatagtctt cggacgcaat
     7381 atctggcaga gcgaagatac gaaaggcatg atagaagccc tgagccacat catccacgaa
     7441 gaccagaaca cgccgtaggg gctggtttca aacccgtcca aaccgccaga cgacgaacgt
     7501 cgctaaggtc cgttctcgaa cgtgaaacaa gtatctctcg cgcaaccctt cagtttctgc
     7561 agacgcgctg cgagagccgt ggcagcgggg tacgaggatg agtagcggtt ggcgagttcg
     7621 cgggggtcgg agcccagctc gtagagttcc ttctcgcctc ccgagtactc gacgtacttg
     7681 cgcttgggaa cgccgccggc gtttacggtg cggacgcccc tgtagggcgg ggagtactcg
     7741 gccgcggcct ccaggaggac ggcgctcctc cagctggtca cggtgttacg gagtacgggc
     7801 cggagggagc gaccgtccac gtaggccggg gtttgtgccc ccgccagcct cgtgaaggtg
     7861 ggcaggtagt cggtgttcag cgccatcttg taggtcgtcg accccggtgc cacgtcggga
     7921 ccgcgcacca gtagggggac gtttacgtcc tcctcgtagg gacgccactt ctgcatcggt
     7981 atacggtgct cgccgtagtg gaacccgttg tccgaggtga agaagatgta ggtgttgtcc
     8041 aactcgccgg cgtcgtccag cgttagcacc acgttctgca ccaggtcgtc tacggcctgc
     8101 aaggactcga cccgcctttc gtggcggttg tctatttcgg tgactcggtc tgcggttagc
     8161 ctgggaagct gcctgatcca ggacggcttg tcgtcgacga tctcctcgtc gaaagaggag
     8221 aagcgcggcc ccgtcacgcc atcgtacgca tgctcgtcgc ggggagccgg ggtagccggc
     8281 gagtgcggag cgatgggggc gacgtaggcg aagaaaggtt gcccttggag tgcgctgtta
     8341 cgcatgaact ctccggcctc gttgctcaac acgtcagtcg tgtaatccgc ttcgttcgtg
     8401 ccgtagtgcg agatggtctg gtcctcgctc acgacatcgt agccgaagta ctggggctcc
     8461 cccgaagtgg cgatggcgaa ccacctgtcc caaccagggg gcacgtaggt ggtgccagcg
     8521 tagcggttca tgtacttgcc aaagagcgcg gtcctgtaac ccgcgtcatc caagcgcgtg
     8581 gccacgttgt cagcctcgtt gcccctggcc tgataggctt gccagccgcc cgtggacgtg
     8641 gaggggctgt cggtcgcgga gttggaccac accccggcgt tgtgggcgta ctggccccgc
     8701 atgatggtgg ccctggaggg cgcgcacagg gcgtgggaga caaaggcgtt ctcgaagctc
     8761 atgccctcac ccttgagcaa cgcgcgagtc tgcggcatgt aactgaggtc gtccttgcgc
     8821 atgtcgtcgg tcaggatgaa cacgatgtta ggacgcgcgg gcaccgtctg ggccgcggcc
     8881 ttgcgcaagt ctaccgagat gcctgaatag gcgccggaga acagcaatcc tacagctaac
     8941 gtagccgacg ccaacaccga gaccactctt cgcattgcat cacccatctt cagcgggccg
     9001 aactcgaaac ggcacgtacc cgtgatccct actcacgcaa cagatgcatc atacacccac
     9061 gcaaaccgct acgcacctat cgggagatca actccgggat ccaacacacc ccgtagggac
     9121 ggttcgcgaa ccgcccaacg aggtcgcgaa cacgtaccgc tcctacaacc accccgcgcg
     9181 tttgaacccg acgtagagaa ggctggagat tgagaacatg atcgcgagcg cgtaggggta
     9241 gccaagaacg gagttcagct cgggcatata gcggaagttc atcccgtaga tgccgcttat
     9301 aagggtggga gcggcgatga tggcggccca ggcactgatc ttctgaacct gcacgttctg
     9361 gcgctgcatg gttgcgttct gccgcacgct cacgatggtc aggttcacgc tgaggatgtt
     9421 ctgcagcagc tccctgaacc cgtcgatccg ctccgtgatg cgcaacgcgc gatcctgcac
     9481 gccacgcatc cggcgcagca gctcgcggtc ggagccgtgg acttgcgcaa agaggcgctc
     9541 gagaaccccg gacagcggct gggccgcgcg gcggaattcc agcacctcac gcgagagctc
     9601 gtagatcctg cgcgacacgt cgacgtcctc gcccccgaag acctgttcct cgatctcctc
     9661 cacatcgttc tccagcccgt cgatgacggg gtcgtagccg tttacgaccc tgtccaccac
     9721 ggcccaaagc acggcccccg tccccttgcc gagaaacccc gcgtggcctt cgagctcccg
     9781 cctgacacct tccaggctgg aggccctgtc gtggtcgatg gtgacgatga aatccctgcc
     9841 gaggaagacg tggatcccgc cgaactccac ccgctcctct tcctcgaggt aacgcgccgt
     9901 ccttagcact acaagcagcg cctcaccgta acggtcagcc ctcggcctgt ggtgggcctt
     9961 gaccacgtcg cgcacggccg cctctggcag gccgaactcg ccggcaaccg aggcaagctc
    10021 ttcgtcgtcc ggctcgacga gctccaccca ggcgaacccg ccgcgctcca cagcggcggg
    10081 ggcggttcct tccgggtccg gggtccccgt acgctttccg tcgacgtaga tggcgctgtc
    10141 cgtgatcacg ggccgacatt atacgcccac cgtcgaacgc accgcccccg cctgccttcc
    10201 gtcccgttcg acagtatgat ggctggggag gaaacggccc ggaaagacct gaccctggag
    10261 gcttcaccat cagatgagca cagaaccgat cctgatcggg gccgacgtgg gcacgacgaa
    10321 catcaaggtc gtcgccttcg acaggagtgg ccgcgccctg aagagcgcga gctcgccgac
    10381 ccccacccgc tacccgagac cgggcagggc caactacgac cccgaggagc tgtgggcctc
    10441 tttcacggcc accctgcgcc gggtcaccgg cgggctggag agcccgggcc gcgtggcgag
    10501 catagccgtc gccagcgtgg gcgaggccgc cgtcccgctc gaccgggacg gcgagcctac
    10561 ctccgacata atcgcctggt tcgatggtag ggcgagaccg caggcacaga ggctggaacg
    10621 caccgtgggt caggagaggc tcttcggcct taccggcctc tccctgcagc ccatcttcgg
    10681 cctctgcaag ttgctctggc tcaaggagaa cgagccagac gcctactcgc gcacggcgaa
    10741 gtggctcaac gtcgcggact acatggcctt ccgcctgtgc ggcgtggccg ccacggatct
    10801 ctccctcgcc tcgcgcacgc tcgccctgga cctgcagggc cggcggtggg ccgaggggct
    10861 gctcgaggag gttggcatct cccccgaggt cttcgcgacc ctgagggaga gcggctccga
    10921 cctcggcccc gtgacccccg cggcggccag ggcaacgggg ttgcccgagg gggcccgtgt
    10981 cgcggcgggg ggccacgacc acgtctgcgg cgcactcgcg cttggcgtca ccgagagggg
    11041 caccatgctc aactcgctcg gtaccgcgga ggccatcttc ctgcccctgg gtagaccgct
    11101 ggaggacccg aaggttggcg accagggcta cacccagggg gctcacgtgg ctggccagta
    11161 ctacgtattc ggcggccagt acacctcggg ggcgagcgtc gaatggatgc ggaagatcct
    11221 ggacggggct gactacgaga ccctgatctc ggaggccgag gaggtcccgc ccgggtcact
    11281 cggcgccttc ttcctacccc acctgcgcct ggcgaaccca ccctacgacg acccggcggc
    11341 gaggggcgcg ttcgtcggcc tcagcgccga cgtgggccgc ggcgccctct tccgggccat
    11401 gctcgaaggg ctggcctacg actcgcgcaa ctcgctggaa ccactcc
//
LOCUS       DBNGVD010000159         5175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_215172, whole genome shotgun sequence.
ACCESSION   DBNGVD010000159 DBNGVD010000000
VERSION     DBNGVD010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_215172"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC16CM_04485"
     CDS             complement(<1..332)
                     /locus_tag="AC16CM_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3139116.1"
                     /translation="MSESSREARRLDKNSTLRSPRTLLALLVAVAIIVAALLVVLSQL
                     GGGGGSGGDVGEMYAGIPQKGTTLGDGDAPVTLYLYEDFQCPFCGQFDREMFPDLLDD
                     YVRDGEVKV"
     gene            674..1486
                     /locus_tag="AC16CM_04490"
     CDS             674..1486
                     /locus_tag="AC16CM_04490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139117.1"
                     /translation="MQMSDVHVGSGLFRPDLLEATIEESNALEPDLVAVVGDLTMEGY
                     RWEFEEARRYFDRLECPNVIYAMGNHDAKNVGYRHFEEFFGIREGAAEIPVPEGVAKV
                     VALDSTKPDLDEGEVGREHYGWLDSEFRGWDSGPRILMIHHHILAVPGTGRDVNNLRD
                     AGDVMAILRELHVDIVLSGHRHVPYVWSISGVRIVHSGTVSTMRVRGTMPPSYNVIEI
                     DEESVRIMLHQPGKGEEGIEPLASFSRTPVTTSEFYPDFDRFVIYDDLPFLK"
     gene            complement(1525..3102)
                     /locus_tag="AC16CM_04495"
     CDS             complement(1525..3102)
                     /locus_tag="AC16CM_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139118.1"
                     /translation="MRLITDGNRRWWVVVTMALTTLLMTIDFNGLTVALPTIGRDLGT
                     STTGLQWTINAYLLALAAPSVAAGRLADIFGRRRVLLIGTTVFVMGSAGSGLAQADWW
                     LIAARVVQGFGAAAFFAASLSIVSNAFPPDQRSKGIGVWAGIGTVGLAIGPLIGGFLT
                     QTLSWRWFFFVNIPVAVLAIVLTLLVVRESRDEGASSHVDLFGVLTITGGLAALVLAI
                     QQGDTLGWTSVLVIGSVLAALVLLGLFSVVEPRLPEPLVDLGLFANRGYVGANAVAFT
                     QNFGFAALLFFLTLYLQNILHYSPLEAGLVFLVFSAILAVVDSFAGRLAASVGARLPM
                     AVGMALCAVAFLLLAMLGPTSGISLVIVALLVAGVGQALAYTVSTTGGMQAIPEAKAG
                     AASGILSMIRLVGAVLGVAVTGALFKAMENARLTGLLSASGSDIDASERAEIKGLLSG
                     SAAAEDRLTHLAPGVADQVDRVVREAFVHAFDGAMVLCLAVSLTGVLAAFLVAGKSPQ
                     HATEPTPEIPTPETGRT"
     gene            complement(3258..3632)
                     /locus_tag="AC16CM_04500"
     CDS             complement(3258..3632)
                     /locus_tag="AC16CM_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139119.1"
                     /translation="MPTTVRIKPEVITAHRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     HGWVYAGEPDFVNRQIREVIIALPDIAFEPDAIEESVKTVLEKARSEEEREEGRLLLR
                     RAFEKTGQLEEAETFLSDGTGE"
     gene            complement(3754..4998)
                     /gene="hutI"
                     /locus_tag="AC16CM_04505"
     CDS             complement(3754..4998)
                     /gene="hutI"
                     /locus_tag="AC16CM_04505"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3139120.1"
                     /translation="MARIDNISLLIHDLDAAVTPAGSGPLRSRRFGELRVHSPASVAV
                     SGDRIVAVGPPEEVWREVPRGAEFDSLDGRGKVALPGLIDCHAHPAFLGDRAAEFELR
                     SSGAGYEEIHASGGGIRSTVAATRAGSEEELQAAVERHLGWMLEHGTTTVEAKSGYGL
                     DLETELKSLRAIAAAGEGSPVDVCPTFLGAHTVPPEFEDAAGYLEFVIHEVLPQAAPL
                     ARFADVFLERGSFEAPEAGRYLQACRERGLILRLHADQFSQRGAIPLAVKLGARSVDH
                     LEATGEEEARLLANSDVAAVLLPACGLFLDLPLPPARLLADEGAIVALATDFNPGSSF
                     CESLPLVMNLACTRLGLSPAEALCACTSNAAHVLGLRDVGRLAPGYRADVLILDAPDW
                     RHIAYHLGGDRFAARIKSGQVL"
     gene            complement(4982..>5175)
                     /locus_tag="AC16CM_04510"
     CDS             complement(4982..>5175)
                     /locus_tag="AC16CM_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139121.1"
                     /translation="GWGTRLMQALVARFPGKAWSIPQLVPEELAPTYFTRYGWKQMDT
                     NQQEMVLDLTGRSGRGQDR"
BASE COUNT          952 a         1726 c         1654 g          843 t
ORIGIN      
        1 accttgacct ccccgtccct gacgtagtcg tcgagcaggt cggggaacat ctcgcggtcg
       61 aattgcccac agaacgggca ctgaaagtcc tcgtagaggt acagcgtaac cggggcatca
      121 ccatcgccca gcgtcgtgcc tttctgcggg atgccggcgt acatctcgcc cacgtccccg
      181 ccggaaccac cgcccccacc gagctgactc aacacgacga gcagggcggc cacgatgatg
      241 gcgacggcga ccaggagagc cagaagcgta cgcggggacc gtagcgtgct gttcttatcc
      301 aaccgtctcg cctcccgtga agactcactc aagaagtcaa ttgtaatacg tacggggtac
      361 ctctccgcca gcaagtacca gggagcctga cgtatcagaa gaacgaggac cacaagaccc
      421 ttgccgagag gatcgccacg gggacgatgg ccgaccccgg ctgcccgagg tctcgcggga
      481 agtccggccg gtttcgggct tctgggtggt tgctgggttg gtccggtgat gccgggcgct
      541 agcgtccgtc cctctcccgc gctaccctgt tggacatgaa cgtatggtac tggagccaaa
      601 atgcggttcc gttgaaccag gacggcgggc cgtcgaccgg agatagaacg aatcggggga
      661 cgttttgagg atagtgcaga tgtctgacgt acacgtgggc tctgggttgt tcaggcccga
      721 tctcctggag gccaccatcg aggagagcaa cgctctcgag cctgacctcg tcgccgtagt
      781 gggggacctg acgatggagg gttaccgctg ggagttcgag gaggcaaggc ggtacttcga
      841 ccgtctggag tgccccaacg tcatctacgc catgggcaac cacgacgcca agaacgtcgg
      901 ctaccgccac ttcgaggagt tcttcggtat aagggaaggt gcggccgaga tccccgtacc
      961 ggagggtgtg gccaaggtcg tcgcgctcga ctcgaccaaa cccgacctcg acgaggggga
     1021 ggtcggccgc gagcactacg gatggcttga ctccgagttt cgcgggtggg atagcggacc
     1081 ccgtatactc atgatccacc accacatact cgccgttccc gggacgggcc gcgacgtcaa
     1141 caacctgcgc gacgccgggg acgttatggc catcctacgc gagttgcacg tggatatcgt
     1201 tctctccggc cacaggcatg ttccttacgt gtggagcatc tcgggggtca ggatagtcca
     1261 ctccggtacc gtctcgacca tgcgggtcag gggtaccatg ccgccctcct acaacgtgat
     1321 cgagatcgac gaggagagcg tgaggatcat gctgcaccag cctggcaagg gcgaagaggg
     1381 tatcgaaccc ctagcctcgt tctcccgcac ccccgtaacc acgagcgagt tctaccccga
     1441 cttcgaccgt ttcgtgatct acgatgacct gccgttcttg aagtagggac gaatcgcgga
     1501 ggcttggcac ctccgcgaac tgctctacgt ccgcccggtc tcgggcgtgg ggatttcggg
     1561 cgtgggctcg gtggcgtgtt ggggggattt tccggccacc aggaacgccg ccagcacgcc
     1621 ggtgagcgat acggccaggc agagaaccat cgcgccatcg aaggcgtgca cgaacgcctc
     1681 gcggaccacg cggtccacct ggtcggccac accgggggcg aggtgggtga gtctgtcctc
     1741 ggcggcggca gaccccgaca gcagcccttt tatctccgcg cgttccgatg cgtcgatgtc
     1801 tgaccccgag gccgagagca ggccggtcag cctggcgttc tccatcgcct tgaagagggc
     1861 ccccgtcacc gccaccccga gcacggcgcc gactaacctt atcatggaga gtatccccga
     1921 ggccgcgcca gccttggctt cgggtatcgc ctgcatgccc cccgtggtcg agacggtata
     1981 ggccagggcc tgccctacgc cggccacgag gagcgcgacg ataacgagcg agatgccgct
     2041 cgtggggccc agcatggcca gcagcaggaa cgccacggcg cacagcgcca tgccgacggc
     2101 catcggcagc cgcgccccga ccgacgccgc cagtcgtccg gcgaacgagt cgaccaccgc
     2161 gaggatcgcc gagaacacca ggaacaccag cccggcctcg agcggcgagt agtggaggat
     2221 gttctgcagg tagagcgtga ggaagaacag tagagcggcg aacccgaagt tctgggtgaa
     2281 tgccacggcg ttggccccca cgtagccccg gttggcgaag agccccaggt ccaccagcgg
     2341 ctccggcagt cgcggctcca cgacggagaa caaccccagc aagaccagcg cggcgagcac
     2401 agatccgatg accagcaccg aggtccaccc cagcgtgtcc ccctgctgga tggccagcac
     2461 cagggccgcg aggccgccgg tgatcgtgag cacgccgaag aggtccacgt gggaactcgc
     2521 gccctcgtct ctggattcgc gaacgaccag cagggtcagc acgatggcga ggacagccac
     2581 gggtatgttc acgaagaaga accagcgcca cgagagtgtc tgggtcagga aacccccgat
     2641 caggggtcct atggccagtc ccacggtgcc gatgccggcc cacacgccga tgcccttgct
     2701 ccgctgatcg ggcgggaagg cgttgctgac tatggagaga gaggcggcga agaaagcggc
     2761 cgcgccgaaa ccctggacga cccgcgcggc tatcagccac cagtcggcct gcgccagccc
     2821 cgaacccgct gagcccatga cgaacaccgt ggtgccgatc aggagcaccc tacgccggcc
     2881 gaagatgtca gcgagccttc ccgccgcgac cgatggggcc gcgagggcca gcaggtacgc
     2941 gttgatggtc cactgcagac cggtggtgga ggtgccgagg tcccggccga tcgtgggcaa
     3001 tgcgaccgtg agcccgttga agtctatcgt catgagcagg gttgtaaggg ccatggttac
     3061 caccacccac cacctgcggt tgccgtcggt gatcagacgc atcctcggtc ctccccaaga
     3121 tcgaggcccc acgggccacc taaaagaaag atctaccggc ttctccccga aggataatcc
     3181 cccctcaggt cgcggcgcat cccccaattg ggctagttgc ccgcgctgac cctactccta
     3241 ccgcctccgg cgtcgcgcta ctcgccggtg ccgtccgaaa gaaacgtctc ggcctcctcc
     3301 aactggcccg tcttctcgaa ggcccggcgc aggagcaggc gtccttcttc gcgttcctcc
     3361 tccgaacggg ccttctcgag cactgtcttc acggactctt ctatggcgtc gggctcgaag
     3421 gcgatgtccg gcagggcgat tatgacctcc cggatctgac ggttcacgaa gtccggctcg
     3481 ccggcgtaca cccaaccgtg gcgggccagc acccaggccc agcctttcat cctgatggtg
     3541 ttctctatgt cttcgtcctc gaggccgaac atctcgatcc tgagcctgtg ggcggttatt
     3601 acctctggct ttatgcgtac ggtagtcggc atgagcacag tgtacagcgg atcgcaccgg
     3661 acataagctg acgcgcaagc tctcggcaat agcctccaag tcagaacatg aaagctgaca
     3721 gctgatagct agagaacatc aactcgtcgg cgttcagagg acctgcccgg actttatcct
     3781 ggccgcgaac ctgtccccgc cgaggtggta ggcgatgtgg cgccagtcag gggcgtcgag
     3841 gatgagcacg tcggccctgt agcccggtgc gagcctgccc acgtcccgca ggccgagcac
     3901 gtgggcggcg ttgctcgtgc aggcgcagag ggcctcggcg ggcgagaggc cgagacgcgt
     3961 gcaggcgagg ttcatcacca gcggaagcga ctcgcagaag gagcttcccg ggttgaaatc
     4021 cgtcgccagg gcgacgatgg ccccctcgtc ggccaggagt cgcgctggcg ggagggggag
     4081 gtcgaggaag agcccgcagg ccggaagaag caccgccgcg acatcgctgt ttgccagcag
     4141 tcgggcttcc tcttcccccg ttgcctccag gtggtccacg ctcctggcgc cgagctttac
     4201 ggcaagtggt atcgcccccc tttgcgagaa ctggtcggcg tgcaacctga ggataagccc
     4261 ccgctcgcgg cacgcctgca ggtagcgccc cgcctcgggc gcttcgaagg atccacgttc
     4321 cagaaagacg tcggcgaagc gggcgagggg cgccgcctgg ggtagcacct catggatcac
     4381 gaactccagg taccctgccg cgtcctcgaa ctcgggcggg accgtgtgcg ccccgaggaa
     4441 ggtggggcac acgtcgaccg ggctgccctc tcccgcggct gctatcgccc gcagggactt
     4501 cagctccgtc tcgaggtcga ggccgtagcc ggacttggcc tccacggtcg tggtcccgtg
     4561 ttccagcatc cacccgagat gacgttcgac ggccgcctgt aactcttcct cgcttcccgc
     4621 ccgcgtcgcc gctaccgtgg acctgatgcc gccacccgaa gcgtggatct cctcgtagcc
     4681 ggcacccgag gaacgtagct cgaactcggc cgcccggtcc ccgaggaagg ccgggtgcgc
     4741 gtggcagtcg ataagcccgg gtagcgccac tttcccacgc ccatccaggc tatcgaactc
     4801 ggcccctcgc ggaacctccc gccacacctc ctccggagga ccgacagcaa cgatacggtc
     4861 cccggatacg gctaccgagg cgggggagtg aacacggagc tctccaaatc gcctgctgcg
     4921 gagcggcccg cttcccgcag gcgtaacggc cgcgtcgagg tcatggatca ggagcgatat
     4981 gttatctatc ctggccacgt cccgacctcc ccgtaaggtc aagtaccatc tcctgctggt
     5041 tcgtatccat ctgcttccag ccgtaccgcg taaagtaggt gggcgctagc tcttcgggaa
     5101 ctagctgcgg tatggaccat gccttgccag gaaatctggc aacgagagcc tgcatgagcc
     5161 gggtgcccca tccgc
//
LOCUS       DBNGVD010000160         3015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3494778, whole genome shotgun sequence.
ACCESSION   DBNGVD010000160 DBNGVD010000000
VERSION     DBNGVD010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3494778"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1031
                     /locus_tag="AC16CM_04515"
     CDS             111..1031
                     /locus_tag="AC16CM_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3139122.1"
                     /translation="MQSDAAQSSGKQRSRRRGISFEVKMMLPALVILAAISILPLIYI
                     IWMSLHNVTLLGGSGIESNWTGLDNWGRMFTDSNVWSGWLAMIIYFVATVGLEMVLGL
                     VIALVIHEVAWGKNLALSLVLIPMFIAPVIVGLLGRFLVNPTYGLYAWVLRESGLYDG
                     NILGGSVSAFVAVVLMDVWEWTPLVVLITLAGLVSIQPQVLEAARVDGANYFERLRHI
                     VLPSITGIVIVALLIRSMDALRYFDIIWQATNGGPANATKIIPLRLYESAFRFFHLGY
                     AATIGLLMLAVSILVANLFTGILRQRGLLK"
     gene            1028..1867
                     /locus_tag="AC16CM_04520"
     CDS             1028..1867
                     /locus_tag="AC16CM_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3139123.1"
                     /translation="MTTGARSSAGRVIPYALLILILLWTLVPVLWMLISSFKSLGDVL
                     SPTPLLMFEPTLANYISLVSGGNNVLTYFRSSLLASGISTIIATSLGCLAGYGLARSN
                     FRAKKHLAFWIISTRMAPIAAVILPLFIGFTFLHLINTVWGLVVAYLSFNLPFAIWIM
                     NAFFEDLPPGIEEAARVDGASNFQTFWRIALPMTAPGIVTTAILCLVFSWNDYAFAVT
                     FAGPGSQTLPVAASQLITQTGVDWGQLTAIGTVVALPMVLVGLAVRRYLVRGLTLGAV
                     TGE"
     gene            1891..>3015
                     /locus_tag="AC16CM_04525"
     CDS             1891..>3015
                     /locus_tag="AC16CM_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3139124.1"
                     /translation="MQNKAAVMYGTHDVRLEEVPVPEPGPNEVLVEIRAVGVCGSDVH
                     YYEEGRIGSFVVEEPLVLGHESMGTVVALGENATRHEVGDRVTLEPGVPDGTCRECRA
                     GRYNLCPNVRFFATPPIDGAFTNFVSINEDFAFALPDNLSDNAGALMEPLSVGIWACK
                     KGGVTAGDHVLVTGAGPIGLVAMQVAFAQGATEVTLTDVADERLRIGSRLGATRTLNV
                     AEESLDESGIEADVLIECSGNERALTDGIHRVRPAGVAVVVGMGPGEQTSVPLAFIQT
                     SEILLTGTFRYANTYPTAIDLAASGKVDLDAMVTSSYGLPETEEALQASGKDPANVKP
                     MVIPNGGEGVASGRIVCAGGLWWGGSRTTPTEVGFVVPCGG"
BASE COUNT          517 a          977 c          954 g          567 t
ORIGIN      
        1 caccgtaatc tacagtgagg tctccgcgat gctcaccgcg ggcaagagcc cgaaggacgc
       61 catgctcgac gcaaaagagc agatagacct cgcggtgaaa ggagaaccaa gtgcaatctg
      121 atgccgcaca gagctccggc aagcaacggt cccggcgcag ggggatctcc ttcgaggtca
      181 agatgatgct cccggcgctc gttatcctgg cggccatctc catattaccg ctcatctaca
      241 tcatctggat gagcctccac aacgtcacgc tactgggcgg gtccggcatc gagtcaaact
      301 ggaccgggct agacaactgg gggcgcatgt tcaccgactc caacgtgtgg tcgggttggc
      361 tcgccatgat catctacttc gtcgccacgg tggggctgga gatggtactc ggcctcgtca
      421 tcgccctcgt gatccacgag gtagcctggg gcaagaacct tgcgctctcg ctggtcctca
      481 tcccgatgtt catagcgccg gttatcgtgg ggcttctcgg gaggttcctg gtcaacccga
      541 cctacgggct ctacgcctgg gtgctgaggg agagcggcct ctacgacggg aacatcctcg
      601 gcggttccgt ctcggctttc gtcgccgtcg tgctgatgga cgtctgggag tggacgcccc
      661 tggtggtcct gatcacgctg gccgggctcg tctccatcca gccgcaggta ctcgaggccg
      721 ccagggtgga cggcgccaac tacttcgagc gcctcaggca tatcgtgctg ccctccatca
      781 ccggcatcgt gatcgtggcg ctcctgatcc gatccatgga cgcactcagg tacttcgaca
      841 tcatctggca ggcgaccaac ggcgggccgg ccaacgcgac caagatcata cccctgaggc
      901 tctacgagag cgccttccga ttcttccacc tgggttacgc ggcgaccatc gggcttctga
      961 tgcttgccgt ctccatcctg gttgcaaacc tgttcaccgg tattctcaga cagagggggt
     1021 tgctcaaatg accacgggcg cacgctcatc cgcgggtagg gtcatcccgt acgcgttgct
     1081 gatactcatc ttgctctgga ctctggttcc ggtcttgtgg atgctaatct cctccttcaa
     1141 gagcctcggg gacgtactct cgcccacacc gctcctgatg ttcgagccga cgctggccaa
     1201 ctacatcagc ctggtctcgg gcggcaacaa cgtgctcact tacttccgta gcagcctcct
     1261 tgcctctggg atctcgacca tcatcgcgac cagcctgggg tgcctggccg gctacgggct
     1321 cgcgcgaagc aacttcaggg ccaaaaagca cctggcgttc tggatcatct ccacgcgcat
     1381 ggcccccata gccgccgtga tcctgccgct cttcatcggg ttcacgttcc tgcacctgat
     1441 caacaccgtc tggggcctgg tggtcgccta cctgagcttc aacctgccgt tcgcgatctg
     1501 gatcatgaac gcctttttcg aggacctgcc ccccggtatc gaggaggcgg cccgcgtaga
     1561 tggggcatcc aactttcaga cgttctggcg catcgccctc cccatgacgg cccccggaat
     1621 cgtcaccacg gccatcttgt gcctggtctt ctcctggaac gactacgcct tcgccgtgac
     1681 gttcgccggt cccggaagcc agacgcttcc cgtggccgcc tcgcaactga tcacgcagac
     1741 gggcgtggac tggggccagc tcacggccat aggaacggtg gtcgcgctcc cgatggtcct
     1801 cgtcggcctc gccgtaaggc gctatctcgt aaggggcctg acgcttggcg ccgttacggg
     1861 cgaataagcg tcgacctaga aaggaaccga atgcaaaaca aagcggcggt catgtacggc
     1921 acgcacgacg tgaggctcga ggaggtgccc gtccctgagc cggggccgaa cgaggtcctc
     1981 gtcgagatca gggccgtagg cgtctgcggt tctgacgtcc actactacga ggagggccgc
     2041 atcggctcgt tcgtggtgga agagccactc gttctcggcc acgaatcgat gggcacggtc
     2101 gtggcgctcg gggagaacgc gaccaggcac gaggtgggcg accgggtgac gcttgagccc
     2161 ggtgttcccg atggcacctg ccgagagtgc cgggccgggc gctacaacct gtgcccgaac
     2221 gtgcgcttct tcgccacgcc cccgatcgac ggcgccttca ccaacttcgt gagcataaac
     2281 gaggacttcg ccttcgccct gcccgacaac ctctcggaca acgcgggtgc cctcatggag
     2341 ccgctctcgg tggggatctg ggcctgcaag aagggcggtg tcacggccgg ggaccacgtc
     2401 ctcgttaccg gcgccggtcc catagggctc gttgccatgc aggtcgcctt cgcccagggc
     2461 gcgacggagg tcacgcttac ggacgtggcc gacgaacggt tgcggatcgg tagtcgtctt
     2521 ggcgcgacgc gtaccctgaa cgtggccgag gagtctctgg atgagagcgg catcgaagcc
     2581 gacgtcctga tcgagtgctc cggcaacgag cgcgccctga ccgacggcat ccaccgcgtc
     2641 aggccggcgg gcgtcgcggt cgtggtcggg atgggacccg gcgagcagac gagcgtaccc
     2701 ctcgcgttta ttcagaccag cgagatcctg cttacgggca ccttccgcta cgcgaacact
     2761 taccccacgg cgatagacct cgccgcgtcg gggaaggtgg atctcgacgc tatggtgacc
     2821 agcagctacg gcctccccga gaccgaggag gcgttgcagg cgagcggcaa ggatcctgcc
     2881 aacgtcaaac cgatggtgat accgaacggc ggagaggggg tcgcgagcgg gcggatcgtg
     2941 tgcgcgggcg ggttgtggtg gggcggttcg cgaaccaccc cgacagaggt tgggttcgta
     3001 gttccatgtg ggggg
//
LOCUS       DBNGVD010000161         2286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1490562, whole genome shotgun sequence.
ACCESSION   DBNGVD010000161 DBNGVD010000000
VERSION     DBNGVD010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1490562"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..597)
                     /locus_tag="AC16CM_04530"
     CDS             complement(325..597)
                     /locus_tag="AC16CM_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139125.1"
                     /translation="MTLGRGRPPLVPDPGQQVILLNEDGSTIEGFRAVTGPISTETGA
                     IIIRVAVDQEFRDARKEGRRAVSMAWPAERVEVFVPWYKWQRWFTR"
     gene            complement(736..1386)
                     /locus_tag="AC16CM_04535"
     CDS             complement(736..1386)
                     /locus_tag="AC16CM_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3139126.1"
                     /translation="MRVLIAGAHGKTARRLTRMLVAEGHEVRGLVRKEDQTEDVASDG
                     AEPVLVDLEAEEVSGGVGEAVEGCDAIVFAAGAGPGSGDARKETMDYGGAAKLVEAAE
                     VRGVRRYLMLSAMGAADPEQGPEAMRPYLRAKARADERLAQSGLDYTIIRPGGLTEDE
                     GTGRIDAAESLGRRGEIPREDVASTFAAALQDHGTYHKTFDILSGDTPIPDALARL"
     gene            complement(1494..1871)
                     /locus_tag="AC16CM_04540"
     CDS             complement(1494..1871)
                     /locus_tag="AC16CM_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139127.1"
                     /translation="MGVLNLGYPLWLLAAVLVVLGALTFYVLRGRVSDRLLVPLTIAL
                     TPVSVVLVVAVAVLISSLLSPFYEDPSEGLRERPQNTPTATPEPTAPTTSPSASPDTS
                     PSATSSASATATGSATATASPSP"
     gene            complement(1871..2029)
                     /locus_tag="AC16CM_04545"
     CDS             complement(1871..2029)
                     /locus_tag="AC16CM_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139128.1"
                     /translation="MRIPPRGYVTLALLCGELVCVIGWAFTGIFFILVVGILAGVGSV
                     LAAMRWED"
BASE COUNT          391 a          790 c          732 g          373 t
ORIGIN      
        1 gcgacctcca ccacgagatc gaagatctct agcacgctcc ggctaacgcc tacgctctca
       61 gcacggccct tcgggccttt cagctcgctc ccttcggtcg ctttcagcca gtcgtctcga
      121 ttcgtaacgg tggctcagac aacccacctc ttccgggcag cgaatctcta gagcggaagg
      181 gggcaggccg cagtgggatc tagccgaaag cgagccaggc gaagccgggc gaagcgttgc
      241 tgacaagcga ggcgaagccg agcgtgctga caaacggagc ccggagggcg tagtgtgctg
      301 acaagcgagg cgaagccgag cgtgctatct cgtgaaccat ctttgccatt tgtaccaggg
      361 cacgaatacc tccacccttt cggcgggcca ggccatgctc acggcgcggc ggccttcttt
      421 gcgggcgtcc cggaactcct ggtcgacggc gaccctgatg atgatcgcac ccgtctccgt
      481 ggatatcggc cctgttacgg cgcggaaccc ctcgatcgtc gaaccgtcct cgttcagcag
      541 gatcacctgc tgcccggggt ccggtacgag ggggggtctc cccctgccca acgtcatctc
      601 tctatcgctc gcatccctgg tctccagggg acaagatatc ccaaacgccc acgacccgcc
      661 acctgacccc gaggagtcca ccagaacacc gggaaatgcc ccggtcgttc atccagagag
      721 accgtaacgt gcccttcaga gccttgcgag cgcgtccggg atcggggtgt ctccggatag
      781 gatgtcgaag gttttgtggt acgtgccgtg atcctggagc gcggcggcga aggtactggc
      841 cacgtcttcg cgggggatct cccccctccg ccccaggctc tcggctgcat ctatgcggcc
      901 tgtgccctcg tcctcggtga ggccgccggg acggatgatg gtgtagtcga ggccgctctg
      961 cgcgaggcgc tcgtcggccc ttgccttggc gcggaggtag gggcgcatgg cctcaggacc
     1021 ctgctcgggg tcggccgcgc ccatggcgct gagcatgagg tagcggcgaa ccccgcggac
     1081 ctccgcggcc tcgaccagct tggccgcccc gccgtaatcc atcgtctcct tgcgcgcgtc
     1141 gccgctcccc ggcccggccc cggcggcgaa aacgatggcg tcgcaaccct ccaccgcctc
     1201 tccgaccccg cccgagacct cctctgcctc gaggtcgacc agcacgggct cagccccgtc
     1261 agaggctaca tcctccgtct ggtcctcctt gcggacgagc cccctcacct cgtggccctc
     1321 cgcgacgagc atcctcgtca agcgcctcgc cgtcttgccg tgcgccccgg cgatcaaaac
     1381 cctcacgcca acctacctcc ctgaaagtcg ggtcctcccc cagtgtaacc cggcgcccgg
     1441 gggctgtgac ggagagagcc ggagaaggcg tcgggctccg gttgcgcgcc cgttcaggga
     1501 gaggggctcg ccgtggccgt ggccgagccg gtggcggtgg cagaagccga ggaagtggcg
     1561 ctcggggagg tgtcaggaga ggccgacggg gacgtggtgg gggccgtcgg ctccggggtg
     1621 gccgtcgggg tattctgggg ccgctccctc aggccctcgc tcgggtcttc gtagaacggc
     1681 gacagcaggg aggagatcag cacggctacg gccaccacga gcacgacgct tactggggta
     1741 agggcgatcg tcagaggaac gagcagccgg tccgacacgc gtccgcgcag cacgtagaac
     1801 gtgagcgcgc caagcaccac gagcacggcc gcgagcagcc acaaagggta tcccaggttg
     1861 agcacgccca ctagtcctcc cagcgcatcg ccgcgagcac cgagcccacg ccggccaata
     1921 tgccgacgac gagtataaag aagatgcccg tgaacgccca gcctatgacg cagactagtt
     1981 caccgcagag cagcgccaac gtcacgtatc ccctgggcgg gatcctcatc gagcctctac
     2041 ttcctctcct cggtaaccca gtggacgcgg cctggaagaa gatcatccct gcaacgatcc
     2101 ggtggcggac acatcattgt agtacgggag gtgccaacaa aatgtttcga tggggaagtg
     2161 cggcccccac ggcctgctgc gatgcccgag aagaggctac cggctgcagg cttcgggacg
     2221 gtcctggacc tgatgcccgt tgcctgtagg ctgtgatcat ctcgggttca ctctctccga
     2281 agcgac
//
LOCUS       DBNGVD010000162         3084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4041543, whole genome shotgun sequence.
ACCESSION   DBNGVD010000162 DBNGVD010000000
VERSION     DBNGVD010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4041543"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16CM_04550"
     CDS             <1..326
                     /locus_tag="AC16CM_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3139129.1"
                     /translation="GPALAKELIFTGRRIGAEEAREIGLANRVVGRGEALGAARELAA
                     EIGRNAPLAVRHAKTAANRALDVDLVSGLEYETDQFALLFATEDALEGMNAFGEKRDP
                     KFEGR"
     gene            341..721
                     /locus_tag="AC16CM_04555"
     CDS             341..721
                     /locus_tag="AC16CM_04555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3139130.1"
                     /translation="MRVRYAEIDAQGHVNNATYLSYFEVGRVEWLRSTGQSYREMERQ
                     GRGLVVVEALLKYRRPAYFDDELTLRTTLSELGRVSLRFDYEVLREAESLVTGHTRHA
                     CIDLATGKPVRMPAPLLELVPRGG"
     gene            833..1588
                     /locus_tag="AC16CM_04560"
     CDS             833..1588
                     /locus_tag="AC16CM_04560"
                     /inference="COORDINATES: protein motif:HMM:NF027034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR3139131.1"
                     /translation="MNPLAAQHAEWLLESGVAGTCTSHGAENNLYKMGLLLEHDENNT
                     FGMEDLLTGVTFEEAYEAVTHQLGNPPDMEETPGRGCIDPSRTATGLLEAGERIGNVA
                     RSGGRLIFATGHPGAMILYYLGLVRWAEELGGTVVTAAPRGRHQKGISLDWADRVGAL
                     GDGASLFHTHDPDPMRDVLGQSGPLDLVVADHGFAGAAIAAGVPTVVVMDTNDPAFAV
                     VAARGADVTVVPMDDNRPLNSYAAALEVLKGGS"
     gene            complement(1712..>3084)
                     /locus_tag="AC16CM_04565"
     CDS             complement(1712..>3084)
                     /locus_tag="AC16CM_04565"
                     /inference="COORDINATES: protein motif:HMM:NF052715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139132.1"
                     /translation="ARDLPERQRTMRSTLDWSHDLLHAPEKELFRRLSVFVGGWTLEA
                     AESVGADGLVDAEDVFAFLGNLIEQSLVETEGMADGTVRYGMLEPVRQYALEKLGDCG
                     EEAAMRRRQASFYLRFMEESGPGLRGRDQASLLRQMEAELGNLRVALRFSTENGGAQE
                     IARASWTSWTYWWLSGHLSEGRGWMEEALASESNMSESSRATLLFVAATLGQALGDIE
                     STQGMIEESMRIYEGLGDNIGYYYASGTAGLIAVGQGRYEEGLALMEESGQRRLEMGD
                     PWPASAMFGFSATVALRLGEGARARLLAERALSLAREVGARELITGALPTLATTARSD
                     GDLGPAARLFEEGLVLSAEVGDKSNVAYYLEALAEISVEENELDRAARLWGAAGALLE
                     TIEIISYPHATDRTLYDRQLASARQRLGEQAWEKAAREGRAMTPEEAVSYALERDSAL
                     EPPTSR"
BASE COUNT          527 a         1101 c          937 g          519 t
ORIGIN      
        1 tcggcccggc cctggccaag gagcttatct tcaccggccg ccgcatcggg gccgaagagg
       61 cgcgtgagat cgggctcgcc aaccgcgtcg tgggcagggg cgaggctctc ggcgccgcca
      121 gggaactggc cgcggagata ggccgcaacg cccccctcgc cgtcaggcac gccaagaccg
      181 ccgcgaaccg ggccctcgac gtggatctcg tgagcggcct ggagtacgag acggaccagt
      241 tcgccctgct cttcgccacg gaggacgccc tggaaggcat gaacgccttc ggcgagaagc
      301 gcgacccgaa gttcgaggga agatagtcca cgagacaaca atacgggtcc gctacgccga
      361 gatcgacgcc cagggtcacg tcaacaacgc gacctacctc tcctacttcg aggtcggtcg
      421 cgtggagtgg ctgcgatcca cgggccaatc ctacagggag atggagcgac agggccgcgg
      481 cctcgtcgtg gtcgaggcgc tcctcaagta ccgccgaccc gcctacttcg acgacgagct
      541 gacgctccgc accacgctct ccgagcttgg cagggtctcg ctccgcttcg attacgaggt
      601 gctgcgcgag gcggagtcgc ttgtcacagg ccacacccgc catgcctgca tcgaccttgc
      661 aacgggcaaa cccgtcagga tgcccgcacc gcttctggag cttgtgccac gcggaggttg
      721 agggcggttc gtcgtcgggc ggtttatcgt cgggcggttc gcgaagcgcc cctacatttc
      781 catattcacc tgtacctctc actgtgaacc gactctgcta cactctggcc ccgtgaatcc
      841 acttgccgca caacatgccg aatggctgct cgagtctggc gtagctggta cgtgtacctc
      901 ccacggtgca gagaacaacc tctacaagat gggcctgctc ctcgagcacg acgagaacaa
      961 cacgttcggc atggaggacc tcctgaccgg ggtaaccttc gaagaggcct acgaggctgt
     1021 cacgcaccag ctcggcaacc cgccggacat ggaagagacc ccgggacgcg gttgcataga
     1081 cccgtcgcgc acggccacag gcctcctcga agcgggagag cgcataggaa acgtcgccag
     1141 gtcgggtggt cggctcatct tcgccacggg gcatccgggg gccatgatcc tgtactacct
     1201 ggggcttgtc cggtgggcag aagagctcgg aggcactgtg gtaaccgccg ccccgcgcgg
     1261 acgccaccag aaaggtatat ccctcgactg ggctgaccgg gtgggagccc tcggcgacgg
     1321 cgccagcctg ttccacaccc acgatcccga ccccatgcgc gatgtgctcg gccagtcggg
     1381 acccctcgac ctcgtggttg ccgaccacgg tttcgcaggg gcggccatag ccgccggcgt
     1441 ccccacggta gtggtgatgg acaccaacga tccagccttc gccgtggtcg ccgcccgcgg
     1501 tgccgacgtg acggtcgtgc ccatggacga caaccgtccc ctgaactcct acgccgcagc
     1561 cctcgaagtg ctaaagggcg gctcctgact ccctgccctc ttccaccacc cgggcgttac
     1621 ccgaaacgga agaactcgag cagagcaaag caacaggcgt attttctgta gcctgaaacc
     1681 tgtaaccttc taacccctcc ctggcaagca tctagcggga agtgggtggt tccagggcgc
     1741 tgtccctctc cagggcgtaa ctcacggcct cttctggggt catagccctg ccctctcttg
     1801 ccgccttctc ccacgcttgc tctccgagcc tctgccgggc ggaggcaagt tgccggtcgt
     1861 agagcgtgcg gtcggtggcg tgggggtagg atattatctc tatcgtctcc aggagggccc
     1921 ctgccgcgcc ccagagtctg gccgcccggt ccagctcgtt ctcctcgacg gagatctccg
     1981 cgagagcctc caggtagtag gccacgttgg acttgtcgcc gacctcggcg gagaggacca
     2041 ggccttcctc gaagagccgc gccgccggac cgaggtcgcc gtccgacctg gccgtggttg
     2101 ccagcgtcgg aagcgccccc gtgatcagct ccctggctcc cacctccctg gcgagcgaga
     2161 gggcccgctc ggccagaaga cgggcgcgtg ctccttcacc cagcctcagc gccacggtgg
     2221 ccgagaaacc gaacatggcg ctggctggcc acgggtctcc catctccagc cttcgctgcc
     2281 cggactcctc catcaacgcg agaccctctt cgtagcggcc ctggcccacg gcgatgagcc
     2341 ccgcggtccc cgatgcgtaa tagtagccta tgttgtcgcc caggccctca tatattctca
     2401 tgctctcttc gatcatcccc tgcgtggact cgatgtcgcc gagggcctgt ccgagcgtgg
     2461 ccgcgacgaa caggagcgtc gcccgggaag actcggacat gttagattcg ctagcgagcg
     2521 cctcctccat ccagccgcgg ccctcgctca agtgcccgct caaccaccag tacgtccatg
     2581 aggtccagga cgcgcgcgcg atctcctgcg cccccccgtt ctcggtagag aaccgtagcg
     2641 cgacgcgcag gttgccaagc tcggcctcca tctgccggag aagggaggcc tgatcccgcc
     2701 ccctcagccc cgggcctgat tcttccatga agcgcaggta gaacgaggcc tgacgacgcc
     2761 tcatcgccgc ttcctccccg caatccccca gcttctcgag ggcgtactgc cgcaccggct
     2821 cgagcatgcc gtagcgtacg gtaccgtcgg ccatcccttc cgtctcgaca agagattgct
     2881 cgatcaggtt accgagaaag gcgaacacgt cctcggcatc gaccaggccg tccgctccca
     2941 cgctctcggc cgcctccagc gtccatcccc ccacgaaaac cgagagcctc ctgaagagct
     3001 ccttctcggg ggcgtgcaac aggtcgtggc tccagtcgag ggtgctacgc atcgtgcgct
     3061 ggcgctctgg aaggtccctc gccc
//
LOCUS       DBNGVD010000163         3578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1490820, whole genome shotgun sequence.
ACCESSION   DBNGVD010000163 DBNGVD010000000
VERSION     DBNGVD010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1490820"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16CM_04570"
     CDS             complement(<1..644)
                     /locus_tag="AC16CM_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139133.1"
                     /translation="MPIRWRLTIFNALVIATILTLLGVSVFLLVRDALLSGVEYTVRE
                     RAASVARTIETGQTLSASDVDRLTLEGVFVVVRDGDGEILARTVRSGPAGDSGERLWR
                     GAVVGGEPVEGRLEVSPGERGYVYALPVDPPDLGSLSPYLYKALARNREASPPGEVLG
                     EADTIPFPVDARVIEVGKSYEAAGETVSTFAALLAAAILISLLVSAGGGYLLARA"
     gene            complement(648..1325)
                     /locus_tag="AC16CM_04575"
     CDS             complement(648..1325)
                     /locus_tag="AC16CM_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3139134.1"
                     /translation="MKPGTRVLVVEDDRSIARLLELEFEHNGLAVRCAYDGPSGLDLA
                     REFAPSVIVLDIMLPEMDGVCVLKHLRRRGDRSAVLMLTARDAVPDKVHSLKLGADDY
                     MTKPFSTEELPARMGALLRRTEGDEILRVADLYIDTASREVRRDERAIELTAREYELL
                     EFMARNPRRVLSRGLLLSRVWGQDAGVETNVVDVYVGYLRKKLDAGGDEPLIRTLRGA
                     GYVLRAE"
     gene            1561..3255
                     /locus_tag="AC16CM_04580"
     CDS             1561..3255
                     /locus_tag="AC16CM_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3139135.1"
                     /translation="MAGLKADTIPPDFLVPGGMSRRDFLRLGGAGMAGAVLLGTAGCG
                     VFEGGSQGSTGAANPRNITFNLEDTIRDLDSSTTTDSVSTDVLLNVMSGLYRLDPDAQ
                     PVPDLAENVKISEDQLSYTFKLRDGIMWSNGDPVTAQDFEYAWKRVLNPDTGAQYAYI
                     ISTFVKGANAYNTGRGAADDVAVKATDEKTLEVELLAPSPFWLGLTAFYTYLPLNQKF
                     VEQQGEDYALSADALIFNGPYKLTSFKPTQGVTMVKNQDYWNRANVPIERVEGKIVKE
                     LDTAVNLYESGELDVQTIDGEYVGEYKDSPEYHTITFFATFYMVGNQKLPAFQNLNIR
                     KAIQIGYDRDALVNQILKNGSPTAPGLVPFGIAGPGKETFREYVGAVQPEFDPEKARS
                     LFQKGTDELGSNPTIELLAYDDSTARDMATFLQSQFQENLGARINIKVQPFDRKLELE
                     ENGDFQLSWQAWIADYNDPMTFMDLFESTSPFNTYQYSNPEYDKLISRARDELDEERR
                     MDMLRQAEIILVEDDAGTAPMYFQGQARLQKPFITRYVNQPYGGGRDISLWRIRTS"
BASE COUNT          735 a         1211 c         1045 g          587 t
ORIGIN      
        1 gcccgggcga gcaggtagcc cccgcccgcc gagacgagca gcgagatcaa gatcgccgcc
       61 gccagcaggg cggcgaaggt cgagaccgtc tcgcccgcgg cctcgtatga ctttcccacc
      121 tctataaccc gcgcgtccac agggaagggt atggtgtccg cctcgccaag cacctcgccg
      181 ggtggggagg cctccctgtt cctggcaagg gccttgtaga ggtaggggga gaggcttccg
      241 agatccggcg ggtcgaccgg gagcgcgtac acatatcccc gttcgcccgg cgacacctcc
      301 agccggccct caactggttc tccgccgacc acggcccccc gccagagccg ctcgccggag
      361 tcgccggccg ggcccgaacg tacggtgcgc gcgaggatct cgccgtcacc gtcgcgcacc
      421 accacgaaaa ccccttccag ggtgaggcgg tccacgtcgg aggcgctcag ggtctgcccc
      481 gtctctatcg tgcgggcgac gcttgcggcc cgctccctga ccgtgtactc cacgccggag
      541 aggagggcgt cgcgcaccag caagaagacc gaaaccccga gcagggtcag gatggtcgct
      601 atgaccagcg cgttgaagat ggtcaggcgc cagcgtatcg gcactggcta ctcggccctt
      661 agaacgtagc ccgcgcccct gagcgtccgg atcaggggct cgtcaccccc cgcgtcgagc
      721 ttctttctga ggtacccgac gtaaacgtca accacgttgg tctctacgcc cgcatcctgt
      781 ccccagaccc ggctaaggag caggccacga gagagcacgc gccttggatt ccttgccatg
      841 aactccagca gctcgtactc tctggccgtc agctcgatgg cccgctcgtc ccgcctcacc
      901 tcgcggctgg ccgtgtctat gtagaggtcg gccacgcgca ggatctcatc cccctccgtg
      961 cgcctgagca gcgcgcccat gcgggccggg agctcctctg tgctgaaggg tttggtcatg
     1021 tagtcgtcag ccccaagctt gaggctgtgc accttgtcgg gtacggcatc ccgtgccgtg
     1081 agcatgagga ccgcggacct gtccccgcgc cgcctcagat gcttgagcac gcacaccccg
     1141 tccatctcgg gcagcatgat gtccagcact ataaccgagg gggcgaactc cctggcgaga
     1201 tccaggccac tcggcccgtc gtaggcgcat cgcaccgcca ggccgttgtg ctcgaactcc
     1261 agctccagca gcctcgctat ggaccggtcg tcctcgacga cgagaacccg cgttcccggc
     1321 ttcactcgcc tcctcctgcc cgcctaccac attgtacccg accgacatta cggttcccgt
     1381 agtcggcaga gcccacagcc attctgccta cggtttctaa gagcacgatg agaatcggcg
     1441 cggtttctca tcgtgctctt aagacacgct taaccccctc ttagcaaccg cgccgagaat
     1501 gatacccaca gggttggggc cagattgtcc ctgccggagc acactgaaga ggggagcacc
     1561 atggcgggat taaaggcgga cacgataccc ccagatttcc tcgttcccgg cggtatgagc
     1621 cgcagggatt tcttgaggct cggcggcgcc gggatggccg gggccgtgct ccttggtact
     1681 gccggatgcg gcgtcttcga aggtggcagc cagggttcga ccggcgcagc caaccccagg
     1741 aacatcacct tcaacctgga agacaccatc agggatcttg actcttcgac gaccaccgac
     1801 tcggtctcaa ccgacgtcct gctgaacgtg atgagcggtc tataccgtct ggaccctgac
     1861 gcccagcccg tgcccgacct cgccgagaac gtgaagataa gcgaagatca actctcctac
     1921 accttcaagc tgcgcgacgg catcatgtgg tccaacggcg accccgttac cgcccaggac
     1981 ttcgagtacg cctggaagcg cgtcctgaac ccagacaccg gcgcccagta cgcctacatc
     2041 atctcaacct tcgtcaaggg cgccaacgcc tataacaccg gccggggcgc cgccgatgac
     2101 gttgcggtaa aggccacgga cgagaagacg ctcgaggtgg agctactcgc cccctcgccg
     2161 ttctggctcg gcctgaccgc cttctacacc tatctcccgc taaaccagaa gttcgtcgag
     2221 cagcagggcg aggactacgc cctctcggct gatgcgctca tcttcaacgg cccctacaag
     2281 ctcacgagct tcaagccaac ccagggcgtg acgatggtca agaaccagga ctactggaac
     2341 agggccaacg tgcccatcga gagggtggag ggcaagatcg tcaaggagct cgacaccgcc
     2401 gtaaacctct acgagtccgg cgagctcgac gtgcaaacga tagacggcga gtacgtgggc
     2461 gagtacaaag actcccccga gtaccacacc atcaccttct tcgccacctt ctacatggtc
     2521 ggcaaccaga agctaccggc gttccagaac ctaaacattc gcaaggccat ccagataggc
     2581 tacgaccggg acgcgctggt gaaccagatc ctcaagaacg gctcccccac agcacctggc
     2641 ctcgtgccct tcgggatcgc cggccccggc aaagagacct tccgcgagta cgtcggggct
     2701 gtccagccgg agttcgaccc ggagaaggcg agatcgctct tccagaaggg cacagacgag
     2761 ttggggagca accctaccat agagctgctc gcctacgacg actccaccgc cagggacatg
     2821 gcgaccttct tgcagtcgca gttccaggag aaccttgggg ccaggataaa catcaaggtc
     2881 cagcccttcg accggaagct cgagctcgaa gagaacggcg acttccagct ctcgtggcag
     2941 gcctggatcg cagactacaa cgacccaatg acgttcatgg acctcttcga gagtacctcc
     3001 cccttcaaca cctaccagta ctcaaacccc gaatacgaca agctcatctc ccgggccaga
     3061 gacgaactcg atgaggaaag gcgcatggac atgctaaggc aggcggagat catcctggtc
     3121 gaggacgatg ccgggaccgc gcccatgtac ttccaggggc aggccaggct gcaaaaaccg
     3181 ttcatcacga ggtacgtcaa ccagccctac ggcggcggca gggacatcag cctctggagg
     3241 ataagaacaa gctaacttca ggacccgctc caccctccag ccaatacaca aaaaccgcat
     3301 aatacaccga aggcagcctg atatcgtttt cgggcgccgg gtctatcacc gggctctaga
     3361 actctggtaa ggtgggatct ggcacgaggt aggagggagc ctgatttggc cgagacggtg
     3421 gcgcggtccg ggggacgtaa agagcggctc gaggacatac ttgtcgtgga ctgtgacgtc
     3481 cacgtgcacg agtctccagg ggcgctaatg ccctactgtg agatgccctg gagaaaggcg
     3541 ctcgagaaca tcagggacgt caaagagtac tacctgga
//
LOCUS       DBNGVD010000164         5313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4405666, whole genome shotgun sequence.
ACCESSION   DBNGVD010000164 DBNGVD010000000
VERSION     DBNGVD010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4405666"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1582)
                     /locus_tag="AC16CM_04585"
     CDS             complement(104..1582)
                     /locus_tag="AC16CM_04585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3139136.1"
                     /translation="MEESQREKNNLDDSAVLESITSQWWSFSSLIPLLTGALWLWLAA
                     RGGLPAILVGAVPGALLLGTGLSGLLWAVESRTFQFMALGSVLGIILCIPEIMLSGPL
                     TALVLGLGSAVSFMAAGYLALGQHRCPAGVPVPDTGPNLAARAAVNELMMCGLILTSW
                     PVAVGSTAVRVHREAADAYGLFEENGWFSDPTTYHQDPPPLQDPGIETIEHKGRQVEH
                     LSFESLYEPHEDEPGRERWLSYPRNPVAHAYILRHPGGPRPWLMCIHGIRMGTLGKSM
                     IRFQPEYLHEELGLNVIMPVLPMHGPRDSGGLISGERVLSGDVMDTLHTGAQAMWDLR
                     RIISWLRENEQAPAVGALGHSLGGYTVSLLASLEEDLDCVIAGNPAVDPSHLFWTNAL
                     AVATHSLSAEGIREETLKALLRPVSPLLLEPLVPHEGRAIFAGVVDRVVPPVQAHSLW
                     RHWREPRIAWYQGAHQRFIKAPEAREVLEQTLRASDMLPGSG"
     gene            complement(1667..4228)
                     /locus_tag="AC16CM_04590"
     CDS             complement(1667..4228)
                     /locus_tag="AC16CM_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139137.1"
                     /translation="MKLRRFSGLSFRNLRARLQRTILTAVGIVLGVGIVFGVLTLSNT
                     MSGTFQDLFTRAYGSSDLTVTAAGGSGGFDEKVARRVRDAEGVGSAAPRYSFSSSLIL
                     PKKGELPEVRSMRLFGVEPGSAGLATGFELTDGHFPKGRKQLTLDGGSAESAGLNLGD
                     EVTIGTPEGRKKLELVGLLRIPGGSFGGLAFGMVPLSYGQEVFDKRDQISGIAVEAAE
                     GTSVASLRERLDRELGEGLQVQRSETRTQQVTGQLQGFKIALLFFAGTSLFVGAFLVF
                     NALSMTVLERTRELGMLRALGSTRAMITRSVVLEAVLLGTLGSLLGLLFGYGMARGLV
                     YLFGKAFLFEITNLILSPFALVSALVIGVAVTVLAALYPATKAGRVSPVEAMRARSGA
                     TGGDKGWSRGLTVFGPVLGLLLAGAGVPWIYYLARNLSAELEGFVYASGIAAIIAAFL
                     GISLIIPVLVRPLAALFSPALRLLFGVEGRMAAANATRNRARTALTASALMVGISLVV
                     AFSALGGSLLGSIRDYLDSSLGSDYVVQPTDQNSGAGFTTRLPEEIKRVQGVEKTTSI
                     VSSFRQDGKKLDSVFGVDQNYPDIFRVDYAAGSSGSFSKLEDGGALIGKQLAEERGLG
                     VGSRIEIPGPRGRMKYPVEAVLENDVIGGGTGIYLSREALAEDFNETESEFLAVKAKP
                     GSDRAVLERRIEDIVKNYPQFTVYSNAEWKAQIESDFNRQYVFFYAIMGVSVAVSAFG
                     VVNTLSMSVFERTREIGILRAVGMTRLQIGRLIIDEGIVISLIGCLVGVALGSLLGYL
                     FVQGSGAGGFEIDFYYPRLPALAALLSGLFIGVFAGLFPARSAARKSIVEAVQYE"
     gene            complement(4248..4997)
                     /locus_tag="AC16CM_04595"
     CDS             complement(4248..4997)
                     /locus_tag="AC16CM_04595"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139138.1"
                     /translation="MDEGAGIAVETVGLGKVYGSGTNRVRALEDVSLGFPDGEFAAIM
                     GPSGSGKSTLLHILGALDRPSSGRVVVGGTELSGLSDRKLTLLRRERMGFVFQFFNLI
                     PTLTAEENVLLPVLIAGGKPAKYSARLDELVELVGLSDRRTHRPDELSGGEQQRVAIA
                     RALIKRPDIILADEPTGNLDSRTGSEVLGLLKESASRFDQTILMVTHDPRAAAAADRA
                     VFLSDGKVVDEARNPDQDEILERIKTLESEA"
     gene            5113..>5313
                     /locus_tag="AC16CM_04600"
     CDS             5113..>5313
                     /locus_tag="AC16CM_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139139.1"
                     /translation="MNRKIPLLHYAGNPISPCFSTREADTRPEEANIDTYEAMLIVIP
                     ELDEEQVENTIGRFRTIIERTGG"
BASE COUNT          989 a         1752 c         1716 g          856 t
ORIGIN      
        1 tgctgacaag cgaaggcgtc agcctgagcg tgccgacaaa cggaggccga aggccgtagt
       61 gtgctgacga gcgtaggcga agccgaagcg tgcggcgagg gcgtcagccc gagcccggca
      121 gcatgtcgga ggcgcgaagg gtctgttcga ggacttcgcg tgcttcaggg gcctttatga
      181 agcgctggtg ggcgccctgg taccaggcta tgcgtggctc tctccagtgg cgccacaggc
      241 tgtgggcctg gaccggtgga accacacggt cgaccacgcc ggcgaagatg gccctgccct
      301 cgtgcgggac taggggttcg aggaggaggg gggagacggg gcgcagcagg gctttgagcg
      361 tctcctcacg gatgccctca gcgctcaggg agtgggtggc cacggcgagg gcgttggtcc
      421 agaacaggtg tgaggggtct acggccgggt tgcccgcgat cacgcagtcg aggtcttctt
      481 ccagggaggc tagcagggag acggtgtagc cgccaaggga gtggccaagc gccccgacgg
      541 ccggcgcttg ctcgttctcg cgcagccatg agatgatgcg cctcaggtcc cacatcgcct
      601 gggcgccggt gtgcagcgtg tccataacgt cgccggagag gacgcgctcg ccgcttatca
      661 ggccgccgct atcacgcggg ccgtgcatgg gtaggacggg catgatcacg ttgaggccca
      721 gctcttcgtg caggtactcc ggttggaagc ggatcatgct cttccccagc gtgcccatcc
      781 ttatgccgtg gatgcacatc aaccacggcc gaggcccgcc ggggtggcgc aggatgtagg
      841 cgtgggcaac ggggttccga gggtaggaga gccagcgctc gcgccccggc tcgtcctcgt
      901 gcggttcgta gaggctctcg aaagacaggt gttcgacctg cctccccttg tgttctatgg
      961 tctcgatccc ggggtcttgc aggggtggcg ggtcctggtg gtaggtggtg ggatcggaga
     1021 accagccgtt ctcctcgaac agcccgtagg cgtcggcagc ctcgcgatgg actcgcacgg
     1081 cggtggagcc gacggcgacg ggccagctcg tgaggatcag gccgcacatc atcagctcgt
     1141 tgaccgccgc gcgggctgcc aggttcggac ccgtgtccgg cacagggacg ccagcagggc
     1201 accggtgctg tccgagggcc aggtagcccg ccgccataaa gctaaccgcc gagcccagtc
     1261 ccagcaccag cgctgtcagc ggccccgaga gcatgatctc gggaatacac aggataatcc
     1321 ccaggacgga gccgagcgcc atgaactgga acgtcctgct ctccacggcc cacagaaggc
     1381 cggagagacc ggtgccgagc agcagcgccc ccggcaccgc cccgaccagg atggcgggca
     1441 ggccaccccg cgccgcaagc cacagccaca acgcgcccgt cagcaggggt atcagagacg
     1501 agaaagacca ccactgggag gtaatggatt ccaggaccgc gctgtcgtcg aggttgttct
     1561 tctcccgctg gctctcttcc atagagcgat ccttccccac accctgtttg ccgcgaccgg
     1621 atcattctag acgctcatgc cacaagccgt gcagcgcaga accgctttat tcgtactgga
     1681 cggcctcgac gatgctcttg cgggcggcag agcgggcggg gaagaggccg gcgaagaccc
     1741 ctatgaagag gccggagagc agggcggcga gggccggtag cctcgggtaa tagaagtcta
     1801 tctcgaagcc gccggcgccc gagccctgga cgaagaggta gccgagaaga gagcccaacg
     1861 cgacgccgac gaggcagccg atcaggctga taactatccc ctcgtcgatg atgagccggc
     1921 ctatttgcag cctcgtcatc ccaacggccc tgagtatccc gatctcccgc gtgcgctcga
     1981 agacgctcat ggagagcgtg ttgacgaccc cgaaggcgga gacggccacg gagacgccca
     2041 tgatagcgta gaagaacacg tactggcggt tgaagtcgct ctctatctgc gccttccatt
     2101 cggcgtttga gtacaccgtg aactgggggt aatttttcac gatatcttcg atcctgcgct
     2161 cgagtacggc acgatccgag cccggcttgg cctttaccgc caggaactcg ctctcggtct
     2221 cgttgaagtc ctcggcgagg gcctccctgg agaggtagat gcccgttcca cccccgatga
     2281 cgtcgttctc gagcaccgcc tcgaccgggt acttcatcct tcccctggga ccaggaatct
     2341 ctatcctgga acccacgccg agcccacgct cctcggcgag ctgtttgccg atgagggcgc
     2401 caccgtcttc cagcttcgag aacgaccccg agctcccggc ggcgtagtcg acccggaaga
     2461 tatcggggta gttctggtca accccgaaga cggagtccag cttcttgccg tcctggcgga
     2521 aggaggagac gatgctggtc gtcttctcca cgccctgtac ccttttgatc tcttccggca
     2581 gcctggtcgt gaagccggcc ccggagttct ggtcggtggg ctgcacgacg tagtcgctgc
     2641 ccagcgagct gtccagataa tccctgatgg agccgagcag gctgccgccg agggcggaga
     2701 aggccacgac gagcgagatg ccgaccatca gggccgaggc ggtgagagcc gtcctcgccc
     2761 tgttgcgggt cgcgttcgct gccgccatcc taccctccac gccgaagagg agccgtagcg
     2821 ccggcgagaa gagcgccgcc agcggcctga cgaggacggg gatgatcagg gatatgccca
     2881 ggaaggccgc gatgatcgcc gcgatccccg acgcgtagac gaacccttcc agctccgccg
     2941 aaaggttcct ggcgaggtag tagatccagg gcacccccgc cccggcgagc aagagcccaa
     3001 gcacgggtcc gaagacggtc aggccacgcg accagccctt atcgccgccg gttgctcccg
     3061 agcgggcgcg catggcctcg acggggctga ccctccccgc cttcgtggcg gggtaaaggg
     3121 ctgccaggac cgtcaccgcc acgccgatga ccagggccga gactagcgcg aagggggaga
     3181 ggatgaggtt cgtgatctcg aacaggaaag ccttgccaaa taggtagacg aggcccctgg
     3241 ccatcccgta cccgaagagc aaccccagga gcgaccccag cgtaccgagg agtacggcct
     3301 caagcacgac ggagcgggtg atcatggccc gcgtcgaacc gagcgccctg agcattccca
     3361 gctcgcgcgt cctctccaga accgtcatcg acagcgcgtt gaagacgagg aaggccccca
     3421 cgaacagcga ggtccccgcg aagaagagga gcgcgatctt gaacccttgc aactgccccg
     3481 tgacctgctg cgtacgcgtc tcggacctct ggacctgcaa cccttccccg agctcccggt
     3541 ctagcctctc cctgaggctc gcgaccgagg tgccctcagc ggcctcgacg gcgatgccgg
     3601 agatctgatc gcgcttgtcg aagacttcct gaccgtagga caacggcacc atgccgaagg
     3661 cgagcccgcc gaaagacccg cccggtatac gcaggagccc gacgagctcg agcttcttcc
     3721 gcccctctgg cgtgccgatg gtgacctcat ctcccaggtt caaaccggcg ctctcggcgg
     3781 agcccccatc cagggtgagc tgcttgcggc ccttcggaaa gtgcccatcg gtcagctcga
     3841 agcccgtcgc gagcccagcc gatcctggtt cgacgccgaa gagtcgcatg ctcctgacct
     3901 cgggcagttc tcctttcttc gggaggataa gggaggacga gaacgagtac cggggggccg
     3961 ccgaccccac gccctccgcg tcgcgcaccc tcctggcgac cttctcgtcg aacccgccgc
     4021 tgccgccggc cgccgtgacc gtaaggtccg atgacccgta ggccctggta aagaggtcct
     4081 ggaacgtacc ggacatggtg ttggagaggg tgagtacacc aaatacgatg ccaaccccga
     4141 gcactatgcc aacggccgtg agaatggtcc tttgtaacct tgcccgcagg tttcggaagc
     4201 tgaggccgga gaagcgccgg agcttcacgc tcccccttcc ctgggcacta ggcctccgac
     4261 tccagcgtct tgatgcgctc caggatttca tcctggtcgg gattcctggc ctcgtcaacg
     4321 acctttccgt cggagaggaa gacggcccgg tcggctgcgg ccgcggcgcg cgggtcgtgg
     4381 gtcaccatga gtatggtctg gtcgaagcgg gacgccgact ccttgagcaa cccgagcacc
     4441 tcgctcccgg tccgcgagtc aaggtttccc gttggctcat cggccaggat gatgtccggg
     4501 cgcttgatca gggcccgtgc gatcgccacc cgctgctgct ccccaccgga gagctcgtcc
     4561 ggcctgtgag tccggcgatc actcagcccc acgagctcga cgagctcgtc gagtctggcc
     4621 gagtacttgg caggcttgcc gccagcgata agcacgggca acagcacgtt ctcctcggcc
     4681 gtgagcgtgg ggatcaggtt gaagaactgg aagacgaagc ccatccgctc acgcctgagc
     4741 agcgtgagct tcctgtccga gagccccgag agctccgttc cccctacgac cacccgcccc
     4801 gaagatggcc tgtccagggc gccgaggatg tgtaacagcg tgctcttccc cgacccggaa
     4861 ggacccatga tggccgcgaa ctcaccgtct ggaaacccaa gggaaacatc ctcgagagcc
     4921 cgaacccggt tcgtgcctga cccgtagacc ttcccgagcc ccacggtctc gacggcaatg
     4981 ccagctcctt cgtccatggg cagagtatat tcccgcgggg gttcacgacc gtgaacgata
     5041 cgacgctccc accagccgca acaggagaac aagaagctga cggccagaag ctgaaagccc
     5101 atagctagcg tcttgaaccg caagatcccg ctgttacact atgccggtaa tcccataagc
     5161 ccctgcttct cgactagaga agccgacact agaccggagg aggcaaacat cgacacttac
     5221 gaagccatgc tcatagtgat ccccgagctc gacgaggagc aggtggagaa caccatcggg
     5281 cgttttcgca cgatcatcga gcgcacgggc ggc
//
LOCUS       DBNGVD010000165        19833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4588765, whole genome shotgun sequence.
ACCESSION   DBNGVD010000165 DBNGVD010000000
VERSION     DBNGVD010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19833
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4588765"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1059
                     /gene="thrB"
                     /locus_tag="AC16CM_04605"
     CDS             151..1059
                     /gene="thrB"
                     /locus_tag="AC16CM_04605"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3139140.1"
                     /translation="MISVRVPATSANLGPAYDAVGLALSLSMRVGLERSPSPEIEIRG
                     TGASLIPRGPAHPAYRAARFVAEIVGESDAHFRLVQENHIPPTRGLGGSAAALVGGAV
                     AANELFGGQIAPPDLLNIVCELDGHPDNAAPALLGGLVIGTLTPAGVSAVRLDPKDLK
                     VVVAVPDFAVSTTAARSALPESVSHRDAVFNVGRSGLLLGALATGEYGLLRVAMQDRL
                     HQPYRAHLIPGLEEVIEAALAEGAHGACLSGSGPTVLAFAPENRAAKIGSAMQTAFEK
                     QAVETKAWVLDVDLAGARVEPPEEAI"
     gene            complement(1096..1482)
                     /locus_tag="AC16CM_04610"
     CDS             complement(1096..1482)
                     /locus_tag="AC16CM_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3139141.1"
                     /translation="MNLALWIVAGLLAAVFLLAGSTKLFFARERLARAPGGGWALDFS
                     AGFVKALGTVEVLGAVGLLLPALLDIAPVLVPLAALGLGLIMIGAATVEFRRKEFGHV
                     LVNLTYLALIAFLAWGRFFGPSSFIG"
     gene            1830..2048
                     /locus_tag="AC16CM_04615"
     CDS             1830..2048
                     /locus_tag="AC16CM_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139142.1"
                     /translation="MYWYNPKTRCTETVPAPTTDREALALLEDDINTMAFASEYERLR
                     QTGMGIEQALIFTGHEFRLKHLEFRAAR"
     gene            2182..2361
                     /locus_tag="AC16CM_04620"
     CDS             2182..2361
                     /locus_tag="AC16CM_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3139143.1"
                     /translation="MMPGQHGGSLDDHLHGIGTRIYFPVRCSGGMFGVGDRHASMNDG
                     EICGTSVEFAGEVTV"
     gene            2398..2652
                     /locus_tag="AC16CM_04625"
     CDS             2398..2652
                     /locus_tag="AC16CM_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139144.1"
                     /translation="MRETEGAWISHGTATDCPDAMRKACRKASYLLAGEWGLSLEDAF
                     ILLSIRADVGVAQARKPSPFATIARVVMPKLGAIPGPFRL"
     gene            3060..3992
                     /locus_tag="AC16CM_04630"
     CDS             3060..3992
                     /locus_tag="AC16CM_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3139145.1"
                     /translation="MADAGRSSDHNEEAADDARAGLGVGIPGVPKLRSRTLEVFGTIE
                     VAHHQFAPGEIETLPFKGPVVNLHLSAPHRLVQQQDGRRRGGLVGTNDAAITPTGLPG
                     YWRTDAASEDMSMLLEDGFIRRVANETGVEPGGLEVVPLFSAPEPQIERIGLALLSEM
                     ETRGLGGELYAESLATLLALHLLRNHSSLGRGPRRKVGSEGGFSRHALQQATDYMNDN
                     LQTKLTLQEIAGVAHMSPDHFGRSFRAAIGLTPHQYVIQRRVERARSLLTETNLTVTE
                     VAQAVGFSNSSHLAHHVRRLLGVPPSTLRRESTR"
     gene            4187..7747
                     /locus_tag="AC16CM_04635"
     CDS             4187..7747
                     /locus_tag="AC16CM_04635"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR3139146.1"
                     /translation="MCMYENLVGAEKRSGVLNKTLVALALLAMTLTVIAGVASPARAQ
                     EGTTNTITVDSVKDEINPEDGDCTLREAITSANTDRSSGDATRECAPGSSTDPDRIVL
                     SVTGAVNLTSALPTLSGNVRIEGPGTDQLTVKRDSTAGAFRIFTVGEGSVVSISGITI
                     SGGSSTSEFPDNRGGGIFNAGGNLKINDSTISSNSAAFGGGIYNTGGPVTLTDSTLSN
                     NQSSYSGGGIFNGGAGTVTIANSTLSDNSASSYGGGITTRGGTVTVTNSTLSGNSTEG
                     LGGGVYNLGGLAVMEYSTITNNRARSGSGSGLASFGDSATHTEMLSTIISANQGTDVD
                     ITEGSGDSVDSRGYNLIGDGNATGAFNQTGDKSGVDDPRLGPLADNGGPTQTHTLLSG
                     GPAIDAIPKGTNGCGTTFAEDQRGVSRPQGSGCDIGAFELKVPLTVNSTADTNDGACN
                     GDANGCTLREAIYAANASPGADTITFDLGAGAHTITLGSTLPVVTDDDGLTINGQQNQ
                     ITISGNNSVRVFYVNSGARLDLNNLTVANGDSGGGSGGGIYNSGTLGITNSTIRDNSA
                     FYGYRYGGGIYNDGKLSVTNSTISGNSADTGGGIANRQGTLGVTNSTISGNSAYVHGG
                     SIYNYQGTLGITDSTVSGNKASINGGGIYNNGTAALGNTIVANSIRGGSCGGSITDAG
                     GNLDTDGSCVDETVTTSQTTTDPLLDPKGLQNNGGPTQTIALMPGSKAVDAAVDANCP
                     STDQRGVSRPEDGDSDGTPRCDIGAFELDNVAPVAEPDTYSTPEDSTLAGNVLSNDTD
                     DNGDKLTAVLVSGPSNAASFELKADGSFSYTPNTNHNGPDSFTYKADDGTADSNAATV
                     NITLDPVNDRPGFTKGADQAVNEDAGTQTVTGWAKDISPGPPDESGQQVSFDMTNDNK
                     ALFTSEGQPRISSDGTLTYTLAKDASGTATVTVMATDNGGTTNGGQDTSAAVTFTITV
                     NPVNDVPTVGVAAGGQCGTGGTGGRINLTVNDVESAPGSLTLSAISSNQNLVPNSGLS
                     VGGSGASRTLTVSAVANRSGTATITVRAGDDQDSGTVTVTVRVGSDSTDTLGGTPGAD
                     IIFGKNGANTTAGQGGNDLLCGGNGPDVMSGGAGDDTLDGANGNDALRGNAGRDTLRG
                     SAGDDKLTGGTEADNFDGGSGTDTATDFNAAQGDAATNIP"
     gene            7835..10204
                     /locus_tag="AC16CM_04640"
     CDS             7835..10204
                     /locus_tag="AC16CM_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HIR3139147.1"
                     /translation="MTTHVMVIRSLKALPPLGAALLLWVALLGLTPSPAHAAGSCSTS
                     AGVTTCTFGPTGAEDTFKVPDGVSNVHVVATGAPGAPGAAGYYGGTTGSGARVSGDLT
                     VTPGQTLYVEVGGTPTGNNCDRGVACVGGFNGGGSSKSGGGGGGASDVREVARNQSGS
                     LDSRLIVAAGGGGGGQDGLCAPTLRYYFGGAGGSAGSSGGNGMTCLNAFGGTGGQAGG
                     QNAGGLGGKDVGQNGSLGQGGDGGVSGLLTGGGGGGGLYGGGGGGSIGGAMLYNEPYQ
                     SPAGGGGGGSSLVPTDGVATIVESGSSITISYDQPYAAPVTNDDSATTDEDKALVVDA
                     PGVLANDTHAARTTLMAFVTGGPGHGSLTFNSDGSYTYTPDKDYNGEDSFTYQASDGK
                     GGQDTATASITVKAVNDAPVAANDNATTEEDTPVTIPALSNDSDVDGDELALGIFTGA
                     THGTVSDNGDGTFEYTPNKDYNGEDSFLYKARDGASQSYAAGVFIDVTPVNDAPGFEG
                     GADQSTSEDSGTQSITGWATGISAGAFETQTVTFSATNDNNALFTAAEQPRISSDGTL
                     SYTPASNASGKAIVTVKATDDGGTANGGIDTSAAQTFTITVNPVNDTPTMSVLAGSSS
                     QSACLSGTSGRLTLELSDADSNPGDLEPSVASSSDARLVPKSNVAFGGTGGVRTATIS
                     TVPDLTGTSMVRITVSDGQASSNKSVTVKAGGSGGDTLGGTSGADLLLGKNGNDTLGG
                     LGKSDVLCGAGGNDRLTGGGGSDTFGGGSGTDVATDYNATEGDSRANIP"
     gene            10709..12412
                     /gene="ilvB"
                     /locus_tag="AC16CM_04645"
     CDS             10709..12412
                     /gene="ilvB"
                     /locus_tag="AC16CM_04645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR3139148.1"
                     /translation="MENTALNIEYRPRTGSEAMCEALLDEGVEHLFGYPGGAIMPFYD
                     ALPDYPLRHILTRHEQAAAHAADGYARATGRVGVCVATSGPGATNLVTGLATAQMDSV
                     PLVAITGQVGRPALGKDSFQETNVMGMTLPFTKHSFLVEDPDELYPTVRRAFEIARSG
                     RPGPVLIDVPKDVQFAACNDNGYRRLREAHPPVESPGLERAALLLRNARRPLILAGHG
                     VTLGGAEKELRLLAERTGIPVITTLLGISAFPDDHPLYLGWPGMHGQASVNRAIDRSD
                     VLFAVGMRFDDRITGAVHKFAPNAKVIHIDIDPAELGKVVKPTVGIAADAKAAMRTIL
                     GHLEEGPEGEGCRPWREELEKSQEPERRRKESAHTEYGPIQVMEAIKEAAGPNVRLVT
                     DVGQHQMWAAQFFEFTRANSLITSGGLGTMGFALPAAMGVAFAYPDEPVWVIAGDGGI
                     QMNIQELATIREFGLNIKVAVLNNGYLGMVRQWQQFFHNRRYSETPITGPNYEQLTAA
                     YGLSGRTISEGDDVGEAVRWAQEQEGCTFLDFHIDPEANVYPMIPSGMSVAEMIEEDG
                     A"
     gene            12405..12863
                     /locus_tag="AC16CM_04650"
     CDS             12405..12863
                     /locus_tag="AC16CM_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139149.1"
                     /translation="MPESATGVSLNTVEVRFSGGMLALNRLLMTLQNKRMPVANFNLG
                     NDPQGMRATVLLDCPPESALRYTAIISALEDVTEAGPAQTIEVALIETSADWRAIAER
                     SGVEAHENEGTLVATGEPENVEGFLAALGDDVDDLVRMGPVARPDARGGV"
     gene            12864..13886
                     /gene="ilvC"
                     /locus_tag="AC16CM_04655"
     CDS             12864..13886
                     /gene="ilvC"
                     /locus_tag="AC16CM_04655"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3139150.1"
                     /translation="MARVYREGDIGDEITTRKVAILGYGSQGHAHALNLKESGCDVTV
                     GLYEGSKSWSKAEEDGLPVKTIADAARDADVVMMLLPDEKQPGVFATEVGPNLTEGDL
                     MLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYVEGKGMPSLFAVENDASGQA
                     RDTILSYARGIGSGRAGIIETTFAEETETDLFGEQAVLCGGLTALLKAGFDTLVEAGY
                     QPELAYYECVNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTAGPKIIDEAAKERMRV
                     ILSDIQSGKWAKDWVLENQAGGSSFLAMRRREAESQVEKVGQELRALAAEGIETPAGR
                     AGGENL"
     gene            13883..14839
                     /locus_tag="AC16CM_04660"
     CDS             13883..14839
                     /locus_tag="AC16CM_04660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3139151.1"
                     /translation="MSRKAFAASDAEWAYHGGELVRLGDVRLSPATHALNYGTGVFEG
                     IRAYWSEERGTLQVLKLREHYERFEKSCRLLRIDLPMSVDELCDATLEILRRNAPRVD
                     TYIRPLAFKAVESVGVNLSGESELSIFTVPMGNYVELTGLKVCVSSWRRTPDNAIPAR
                     AKCTGSYVNTALAVDEAHRGGYDDAIFLTQDGQVSEASAANIFLVRKGVLITPPVTAD
                     ILEGITRDAVMELAEKELGMPVVERDVDRTELYAADEVFLSGTGFQIAPVVEVDDRPV
                     GTGSTGPVAERLQELYFKAARGGWDEYAHWTVTVEVAEPARQ"
     gene            15020..16561
                     /locus_tag="AC16CM_04665"
     CDS             15020..16561
                     /locus_tag="AC16CM_04665"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3139152.1"
                     /translation="MRRVEIFDTTLRDGEQSPGISLSVEEKVEIARQLARLRVDVIEA
                     GFPITSEGDFESVSRIAAEVAGPRITGLARIHEEDIVRAWEAVQWSGRPRIHTFVGTS
                     DLHIEHQMRSNRNDILQRAGEAVALAKSLCENVEFSPMDATRTDIGYLSEVVAVAVEA
                     GADVINIADTVGYTTPVEFTAFLKELQARVPGLSDRVLSVHCHDDLGMAVANSLAGVE
                     IGAGQVEVAINGIGERAGNASLEEVVMALATRSDFYDVEVGVEKRQLANTSRMISNIT
                     GYDVPPNKAIVGRNAFLHESGIHQDGVLKDRRTFEIMTKEDIGLEGTNIFLGKHSGRH
                     ALKDALEELGYYVEGEVLKRAFIRFKEIADHKKTVTAADLEAIAADEVGSFEGKFVLE
                     SFRVVAATGRQSRAAVTISHAEQGTFEAEAEGEGPVDAVFRAIDAATNIKGRLTDFRI
                     DAVTGGKDALGEVRISVEFEGNDYAGRGLSQDVVEAAARAYVRAVNVYTAGMVGARWD
                     ARVAP"
     gene            16537..17307
                     /locus_tag="AC16CM_04670"
     CDS             16537..17307
                     /locus_tag="AC16CM_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139153.1"
                     /translation="MGRAGSAVEIPERRDSAPPGYESLDGNIPVLHAPRLEVKSREIG
                     EYLQEGYRALSRVLSAGTPEIAAVLVADEDWRDAPRDNALPYPPGLPYFTRSVEPPLL
                     VLPESLSPVFGPKTEAMLPLIVWHELAHAFLLSREVVRTPVWLGEFVPQAASVTVARR
                     AGLPLGAHLSQIEREPGFTVRSFRGPGGPEDQMSFQNLLLVLAEAALDAFGEALLERL
                     FYGLWEQDEIVDEERAEELLAEALGPGGREWLASRPEF"
     gene            17319..18386
                     /gene="leuB"
                     /locus_tag="AC16CM_04675"
     CDS             17319..18386
                     /gene="leuB"
                     /locus_tag="AC16CM_04675"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3139154.1"
                     /translation="MRDSFEILLLPGDGIGPEVVGAAREVIDAAADHFDLEISYTERR
                     IGGIALREEGEAVSGETLDAALASDSVLLGAVGHPDFDDAPVRPEAGLLGLRKHLGAF
                     ANLRPVEAIPALLASSPLKPEVVEGVDVLIVRELTGGTYFGEKEEGEDRASDLCLYTR
                     EEIERVARVAFDAATRRRRRVTSVDKANVMATSRLWRRVVTEVGRDYPRVELDHILVD
                     AAAMYLVQSPGRFDVMLTENLFGDILSDEAAMLPGSMGMLPSASLGEPGSPGIFEPVH
                     GSAPDIAGEGKANPYATILSAAMMFRHTLGRPDVAEAIEQGVSVALEAHYLTPDLGGK
                     KTTEEVTKAVSQWVGAGEGVI"
     gene            18383..>19833
                     /gene="cimA"
                     /locus_tag="AC16CM_04680"
     CDS             18383..>19833
                     /gene="cimA"
                     /locus_tag="AC16CM_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR3139155.1"
                     /translation="MSIELFDTTLRDGTQREGVTLTAEDKARIAYELDSLGIHYIEAG
                     FPASNPKDLEFFESFDAASLSQARLVAFTRARRPGGRAEDDPAVTLLPSLSAPVACIV
                     AKGWKLHVEKVLRTTPEENLESVRDTVSYLADAGKEVIFDAEHYFDGFRDDPAYSLTL
                     LRAAAEAGATTLVLCDTNGGTLPTELKAIVARTVREFPDVRVGIHTHNDAGCGVANAL
                     AAVEAGATHVQGTVNGVGERCGNCDLVTTIANLQLKMGLKMMEEDQLRRLTETSNYVS
                     ELMNLTPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVGPEVVGNVRRTPVGELSG
                     KNSIRAKAEELGLEPGDATEVLGAIKQREYEGYHYEAADASLALLIGRTTGDDTPLFE
                     LETFRIITEKRAGGHTTTEATIKLSVDGHRVISTAEGNGPVNALDGALREAIGPHYPE
                     LKEIHLSNYKVRILDEHRATAATTRVLIDSTDG"
BASE COUNT         3833 a         6483 c         6558 g         2959 t
ORIGIN      
        1 cgagggcagg gcccccgatg gcacggtcgt gagcgtcctg accggccacg ggctcaagga
       61 ccctgacatc gtgctgggcc gcgtgcggct ccccgatccc gtccccgcca cgttcgaggc
      121 cgtaacaagc gtcatggaga ggtaagggcg gtgatctccg tccgcgtccc ggccacgtcc
      181 gcaaacctcg gacccgccta cgacgcggtg gggctcgcgc tgtccctgag catgcgggtc
      241 gggttggaga gatccccttc ccccgagata gagataaggg gcacgggcgc cagcctcata
      301 ccgcgcgggc cggcgcaccc ggcctacagg gcggcgcggt tcgtggccga gatcgtcggc
      361 gagtccgacg cccacttcag gctcgtgcag gagaaccaca tcccccctac cagggggctc
      421 ggcggaagcg cggccgcgct cgttggcggg gccgtcgcgg caaacgagct gttcgggggt
      481 cagatcgccc cccccgacct gctcaacata gtctgcgagc tggacggcca ccccgataat
      541 gccgccccag ccctcctcgg cgggctcgtg atcggaaccc tcacccccgc cggcgtcagc
      601 gccgtccgcc ttgacccgaa agacctgaag gtggtcgtcg cagtccccga cttcgccgtc
      661 tcgaccacgg cggcccgcag cgccctgccc gagtccgtct cccaccgcga cgcggtcttc
      721 aacgtcggcc gctccggcct gcttctcggc gccctcgcca ccggcgaata cggtctcctg
      781 cgcgtggcca tgcaggaccg gctccaccaa ccctaccgcg cccaccttat ccccggcctg
      841 gaagaggtga tcgaggccgc cctggcagaa ggcgcccacg gcgcgtgcct ctccggctcg
      901 ggtccaaccg ttctcgcctt cgcgcccgag aacagggccg caaagatagg ttcggccatg
      961 cagacggcgt ttgaaaaaca agccgtggaa acgaaagcat gggtcctcga cgtggacctc
     1021 gcaggcgcgc gcgtcgaacc tccggaagag gcaatctagg ggtttcgcgg atggtccgaa
     1081 tcgctttgcg tgcggtcagc cgatgaagga ggagggccca aagaaccggc cccacgccaa
     1141 gaaggcgatc agggctaggt atgtcaggtt caccagcacg tgcccgaact ccttgcggcg
     1201 gaactccacg gtagccgcgc cgatcatgat gaggcccagc ccgagggcgg ccaggggcac
     1261 aagtaccggc gcgatatcga gcagggcggg caggagcagg ccaacggctc ccaggacctc
     1321 gaccgtcccg agggccttga cgaagccggc gctgaagtcc agggcccatc ccccaccggg
     1381 ggccctggct agcctctccc gggcaaagaa caatttggtg gagccggcca acaggaagac
     1441 agcggctagc agtccggcaa cgatccacaa cgcgagattc accatcgcct cctcgatcag
     1501 ggggtacctg taccaagaag acaccgcgcg ggcccccgcc gtgacacccc gtcacgcgaa
     1561 agcggacgcc aggatggtgc cggcgttggc tccggggcgg ctagggtcca gaacggtatt
     1621 ccccggaggc tacccggcgt gatcattgcg gcgaagccgc cagagatctc ccgcgccacg
     1681 gttgcgagga ccggatgccg cggactttac cggacgggcc gtctgtctct cgtttgcctt
     1741 aaccctttgt taaccgagaa tttttcgtcc tttaaccttc ggaggagggt tgattgctag
     1801 tatcatgctt gtgggaagaa ggaggacgca tgtactggta caacccgaag acgaggtgca
     1861 cggaaaccgt accggcacca acgacagacc gggaggccct ggccctgcta gaagacgaca
     1921 tcaacaccat ggcgttcgcg tccgagtacg agcggctgcg ccagacaggc atgggtatcg
     1981 agcaggcgct catctttacg ggacacgagt tcaggctcaa gcacttggag ttcagggctg
     2041 ccaggtagtc gaagcagttc gcgagcagcc gtcagcggtc gcgggcctcg gcaggagcag
     2101 aagcccgacc gctgatcgcg ctacagttcc tatataacgg taggcttctc cctgaaaccc
     2161 ttcgccttta cggcgccgat aatgatgcca ggccagcacg gcggtagcct cgacgaccac
     2221 ctgcacggga tcgggaccag gatctacttc cccgtgcgtt gttccggcgg gatgttcggt
     2281 gtcggggaca ggcacgcgag catgaacgat ggcgagatct gcggcacgag cgtggagttc
     2341 gcgggcgagg taacggtctg attcgacctg ctgaagggca agcaggggta ctggcccgtg
     2401 cgcgagactg agggcgcctg gatctcccac ggcacggcca cggattgccc ggacgccatg
     2461 cgcaaggcct gccgcaaggc gtcctacctc ctggccggcg agtggggcct gagcctcgaa
     2521 gacgctttca tcctcctgag catccgtgcc gatgtgggcg tcgcgcaggc acgcaagccc
     2581 tcccccttcg ccaccatcgc ccgcgtggtg atgcccaagc tgggggccat accaggcccg
     2641 ttccgtctgt aggccccggg tgccatatgc atagctagca cggttctgac gaagacacgc
     2701 ccggtccatt aggatcaccg gctcccttac acgtcgtccc cgcaaaagcg gggatccatc
     2761 gcggcgagct tgccatgctt cgggcagtgc tcgaaccgca cgcagggcgg tccccaaaga
     2821 tacttcattt gggggatgca ccgcccatgc gccgcgcata tgctgctagc attatgggag
     2881 cgatgtagag gaggcagccc acgcgaaggt gtggagtgat gcgttccaga ggtctcgctg
     2941 tactccggta tcgtggacct gctcgtgcca tcgcccggcg agtttgcgcg gaggccaggg
     3001 gcgttccttc catctcgtcg aatagaggcg ctagcaagac ctacacaaag gtcgagtgat
     3061 tggcagacgc gggtcgttca tcggaccata atgaggaagc ggcggacgac gcgcgtgccg
     3121 gcctgggcgt gggaataccc ggggtaccga agctacggtc caggacgctg gaggtcttcg
     3181 ggaccataga ggtggcgcac catcagttcg cccctggcga gatcgagacg ctgcctttca
     3241 agggacccgt tgtgaacctg catttgagcg ccccgcaccg gctggtgcag cagcaggacg
     3301 gaaggaggcg cggggggctc gtaggcacga acgacgccgc catcaccccg acgggactgc
     3361 cgggctactg gcgcacggac gccgcctccg aggacatgag catgctcctc gaagacggct
     3421 tcatccgtcg ggtggctaac gagaccgggg tcgaaccagg aggcctggag gttgtcccgc
     3481 tcttcagcgc acccgagccg cagatagagc gcatcgggct cgcgttgctc tcggagatgg
     3541 agacgcgggg cctcggcggc gagctctacg ccgagtcgct ggcgaccttg ctggcgctgc
     3601 acctcctgcg taaccactcg tcgctaggac gtggcccaag gcgcaaggtc gggagcgaag
     3661 gcggcttctc gaggcacgcg ctccaacagg ccaccgatta catgaacgac aacctccaga
     3721 caaaactgac gcttcaggag atagccgggg tggcgcacat gagccccgac cacttcggcc
     3781 gctcgttcag ggcggccatc gggctcacgc cacaccagta cgttatccaa cggcgcgtag
     3841 agcgggccag gagcctgctc acggagacca acctcaccgt cacggaggtc gcccaggccg
     3901 ttggtttctc gaactctagc cacctcgccc accatgtcag gcgcctgctc ggcgtgcccc
     3961 ccagtaccct gcgccgggag agcacgcgct agcatcctcc gttcctcaaa atgcgcggga
     4021 ttctgttcca aaagcgcgag atcctgttag ccggttcata ccgcgctact ggacaattgc
     4081 tgtacatagg ctgcgctgat cttcgggatc aagagaccat gcaagcaggc aaagacagtc
     4141 aggcggagcc gactggctcc aacaacgctg atcgtaggag agagggatgt gcatgtacga
     4201 gaacctggta ggagctgaga aacgttccgg cgtgctcaac aagacactgg tagccctggc
     4261 actgctggcg atgacgctca ccgtgattgc cggggtggcg agtcccgccc gggctcagga
     4321 aggcaccacc aacactatca ccgtcgacag cgtcaaagac gagatcaatc cggaggacgg
     4381 agactgcacg ctcagggagg ccattacctc tgccaacacg gataggagtt ctggggatgc
     4441 cacccgggag tgtgcgccgg gctcaagcac cgatcccgac cgcatcgtct tgagcgtcac
     4501 cggcgcggtg aaccttacga gcgcgttgcc tacactttcc ggcaacgtgc ggatagaggg
     4561 accggggacc gatcagctca ccgtcaagcg tgatagcact gctggtgcct ttcgcatctt
     4621 cacggttggg gagggcagcg tggtctccat ctccgggatc accatctcgg gcggtagctc
     4681 caccagcgag tttccagaca acagaggtgg cggcatcttc aacgccggcg gcaacctgaa
     4741 gatcaacgac tccaccataa gctccaactc tgcagccttc ggcggcggaa tatacaatac
     4801 tggcggtccg gtgacgctca cggactcgac cctcagcaac aaccaatcga gctactccgg
     4861 cggcggcatt ttcaacggtg gcgctggcac ggtgactatc gccaactcga ccctcagcga
     4921 caattcggcg agcagttacg gcggtgggat taccaccagg ggcggtacgg tgacggttac
     4981 gaactcgacc ctcagcggta attcgacgga ggggctcggc ggcggcgtct acaatctcgg
     5041 cggcctcgcc gtcatggaat actccaccat aaccaacaac agggcccgaa gcggaagtgg
     5101 aagcggcctg gcgagcttcg gggacagcgc aacccacacg gagatgctct ccaccatcat
     5161 ctccgctaac cagggcaccg acgtggacat cacggaaggc agcggagact ccgtcgactc
     5221 gagaggctac aacctcatcg gcgacggcaa cgccaccgga gccttcaacc agaccggaga
     5281 taagagcggc gtggacgatc ccagactcgg tcccctcgca gacaacggag gtcccaccca
     5341 gacccacacc ttgctctccg gcggccccgc gatagacgct atcccgaaag ggaccaacgg
     5401 ctgcggcacc accttcgcgg aagaccagcg cggcgtttct cgtccgcaag gcagcgggtg
     5461 cgacatcggg gccttcgagc tgaaggtccc cctgaccgtg aacagcacag ccgacaccaa
     5521 cgatggcgcc tgcaacgggg acgcaaacgg atgcaccctg cgcgaggcga tatacgccgc
     5581 caacgcgagc ccgggggcag acaccataac cttcgacctc ggagctggag cgcacaccat
     5641 cacgctgggc tccacgctgc cggtggtcac cgacgacgat ggcctgacca taaatgggca
     5701 acagaaccag atcactataa gcggcaacaa cagcgtgcgg gtgttctacg tgaatagcgg
     5761 agcacggttg gatctcaaca acctgacggt ggccaatggc gacagcggcg gcggctccgg
     5821 cggcggcatc tacaacagtg gcacgcttgg tatcaccaat tccactatca gggacaactc
     5881 tgccttctac ggctaccgct acggcggtgg catctacaat gacggcaagc ttagcgtcac
     5941 caattccacc attagcggca acagcgccga cacaggcggc ggcatcgcca acaggcaagg
     6001 cacgcttggc gtcaccaatt ccactattag cggcaactct gcctacgtcc acggcggcag
     6061 tatctacaac taccagggca cgcttggcat taccgattcc accgttagcg gaaacaaggc
     6121 ttccatcaac ggcggcggca tctacaacaa tggcacggcg gcgcttggca acacgatcgt
     6181 tgcgaacagc attcggggag gcagttgcgg cgggagcata accgacgcag gcggcaacct
     6241 cgacactgac ggttcttgcg tagacgaaac cgttaccact tcccagacta ctaccgaccc
     6301 cctgctcgac cccaagggct tgcagaacaa cggcggtccc acccagacca tcgccctcat
     6361 gcccggtagc aaagccgtag acgcggcggt ggatgcaaac tgccccagca ccgatcagcg
     6421 cggcgtgtcc cgtccggagg acggcgacag cgacggcacg ccacgctgcg acatcggcgc
     6481 cttcgagctg gacaacgtgg ctcctgtggc cgagcccgac acctactcca cacccgagga
     6541 ctccactctt gccggcaacg tcctctcgaa cgacacggat gacaatggcg ataagctcac
     6601 ggctgtgctg gtcagcggcc cgagcaatgc cgcctctttc gagctcaagg cggacggttc
     6661 gttctcttac acccccaata ccaaccacaa cggtcccgac tcctttacct acaaggccga
     6721 cgacgggaca gctgactcca acgccgcgac ggtgaacatc accctcgacc ccgtcaatga
     6781 caggcctggt ttcacgaaag gggccgacca ggccgttaac gaggacgcgg gaacgcaaac
     6841 ggtcacgggc tgggccaagg acatctcacc cggtcctccc gacgagtcgg gccagcaggt
     6901 aagcttcgac atgaccaacg acaacaaagc tttgttcacc tcagaggggc aacccaggat
     6961 ctcctccgac ggcacgctca cctatacgct agccaaggac gccagcggca ccgcgaccgt
     7021 cacggttatg gccacagaca acggcggcac gaccaacggt gggcaggaca cctctgcggc
     7081 ggtgaccttc accatcaccg tcaacccggt caacgatgtc cccacggtgg gggtagcggc
     7141 cggggggcag tgcggcacgg gcgggaccgg cgggcgcatc aacctcactg tcaacgacgt
     7201 ggagagcgca ccgggctctc tcaccctcag cgctatctcc tcgaaccaga acctggtgcc
     7261 aaactccggc ctctcggtgg gcggcagcgg cgcgagccgg acgctaacgg tgagcgcggt
     7321 ggccaatcgc tcgggtacgg ccacgatcac cgttagggca ggcgatgatc aggacagcgg
     7381 cacggttacc gtcacggtca gggtcggaag cgactcgacc gacaccctgg gcggcacccc
     7441 tggggccgac atcatcttcg gcaagaacgg cgcaaacacc acggccggac agggcggaaa
     7501 cgacctgctg tgcgggggca acggcccgga cgtgatgagc ggcggcgcgg gagatgacac
     7561 cctcgacggg gccaacggca acgacgcgct gcgcggtaat gcgggtaggg acaccctgcg
     7621 cggcagcgcg ggcgatgaca agctcaccgg cgggacggaa gcggacaact tcgacggcgg
     7681 ttcgggcacg gacaccgcca ccgacttcaa cgctgctcag ggggatgccg caacgaatat
     7741 cccctaaccc cacgatggga gcagcgagta gcagagcggg aggcgacggc actttacccg
     7801 tcgcctcctc gacaagtaga ggagtgcaga gcagatgacg acacacgtga tggtgatacg
     7861 ctcgctcaag gcattgcccc ccctgggggc ggcgctgctg ctgtgggttg cgctgctagg
     7921 cttgaccccg agccccgccc acgcggcggg tagttgctcc acctctgccg gggtgacaac
     7981 gtgcaccttc ggcccgacgg gggctgagga caccttcaag gtccctgacg gggtgagcaa
     8041 cgtccacgtc gtcgcaaccg gcgcacccgg cgcgcccggc gccgcgggat attatggtgg
     8101 cactaccggt agcggggcca gggtgagcgg ggacctgacg gtcacgccgg gccagaccct
     8161 ctacgtggag gtcggcggca ccccgaccgg aaacaactgc gatcggggtg ttgcctgtgt
     8221 cggtgggttc aacggcggtg ggtcgagcaa atccggtgga ggcgggggcg gcgcctccga
     8281 tgtgcgcgag gttgcgagga accagagtgg aagccttgac tcgcgtctga tcgtggccgc
     8341 cggaggtggg ggcggcggcc aggatggtct ttgcgcgccc accctcagat actacttcgg
     8401 gggtgcgggc gggtctgcag ggtccagcgg cggcaacggt atgacgtgcc tcaacgcctt
     8461 cggaggcacc ggtggacagg cgggcggcca gaacgccggg ggtctcgggg gcaaagacgt
     8521 aggccagaac gggtcgctgg ggcagggcgg cgatggcggg gttagcgggc ttctcaccgg
     8581 tggcggtggc ggcggcggcc tgtacggcgg gggcggcgga ggcagtatcg gcggcgctat
     8641 gctctacaac gaaccctatc aatcgccagc gggtggtggc ggcggcggct ccagcctggt
     8701 gccgaccgac ggggtcgcca ccatcgtcga gagcggctct tcgatcacca tcagctacga
     8761 ccagccctac gcggcgccgg tcactaacga tgactctgcc actaccgatg aggacaaggc
     8821 gctggtcgtt gacgctccgg gtgtgctcgc caacgatact cacgccgcca gaaccacgct
     8881 catggcgttc gtgaccggcg gtcccggcca cggctcactg accttcaact ccgacggctc
     8941 ttacacctac acccccgaca aggactacaa cggtgaggac tccttcacct accaggcctc
     9001 cgacggcaaa ggcggtcagg acacggccac ggcctcaatc accgtcaaag ccgtcaacga
     9061 tgccccggtg gcggccaacg acaacgccac cacagaggag gacacccccg tcaccatccc
     9121 ggcgctctcc aacgactcgg acgtggacgg cgacgagctc gcccttggta tcttcaccgg
     9181 cgccacgcac ggcacggtga gcgacaacgg ggacggcacc ttcgagtaca ccccgaacaa
     9241 ggactacaac ggggaagact ccttcctcta caaggcccgc gacggcgcct cgcagagcta
     9301 tgcggccggg gtgttcatcg acgttacccc ggtcaacgac gcgcccggct tcgagggggg
     9361 agcggatcag agcacgtccg aggactcggg gacgcaaagc atcacgggct gggccacggg
     9421 catctcggcc ggagcattcg agacccagac ggtgaccttc agcgcgacca acgacaacaa
     9481 cgctctgttc accgcagcgg agcagcccag gatctcctca gatggcacgc tcagctacac
     9541 tccagccagt aatgccagcg gcaaggccat cgttacggtc aaggcaaccg acgatggcgg
     9601 cacggccaac ggcggaatcg acacaagcgc cgcgcagacc ttcaccatca ccgtcaaccc
     9661 ggtcaacgac acgccaacca tgtcggtgtt agcgggcagc tctagccaga gcgcatgcct
     9721 gagcggcacc agcgggcgcc tcaccctcga gctctccgac gcggacagca acccaggcga
     9781 tctcgagccg agcgtcgcaa gctcctcaga cgccaggctc gtgccgaaga gcaacgtggc
     9841 tttcggcggc acgggcggtg tccgtaccgc cacgatctcc acggttccgg atctcaccgg
     9901 gacctcgatg gtgaggatca cggtcagcga cgggcaggcg agcagcaaca aatccgtgac
     9961 ggtgaaggct gggggcagcg gcggggacac gctcggcggc acgagcgggg ccgacctcct
    10021 gctgggcaag aacggtaacg acacgctagg cggcttgggc aagagcgacg ttctgtgcgg
    10081 ggccggtggc aacgacaggc ttaccggcgg tggtggctct gacaccttcg gtggcggttc
    10141 aggcaccgac gtggccaccg actacaacgc cacggagggt gattccaggg cgaacattcc
    10201 ctgaacacag gaacagcccc tgaatacatc catcgccgag ccaacgaaaa tgcaagcacg
    10261 cctccgccgt ccctggaggt aaaagggtac ggtggaggcc tcgttcggag aaaccggtga
    10321 cgcagcgccg ggacgacgcg gacgagatct cccttgtggc gagaaccacg ctggccccgg
    10381 ccgaatacga acctatgtgc aacttgacaa gcctctaccg ttgtgtaacc cttcaaccca
    10441 cttgcgggct acatgagcga ggactgttga gaaaggtctc caagacggcg cacagagagg
    10501 tctcgggctt gatgctcaag atctcgcgtc tgctcgctcc acccatccac ccgcgagtat
    10561 ccacggtagc cgtaccctcc gccctacgac taattagccg ggcgggccgg ggcggcacgc
    10621 tccggttcgc ctagagccgc aagacgggca gccccgttct tcggcaaggc cggaacagaa
    10681 atacttctct tcatgaatgg ggtaaaccgt ggaaaacaca gcacttaaca tcgaatacag
    10741 gccgcgcacg ggtagcgaag caatgtgcga ggcgctactc gacgaagggg tcgagcacct
    10801 cttcgggtac ccgggcggcg cgatcatgcc cttctacgac gccctgccgg attatcccct
    10861 gaggcacatc ctgacccgcc acgagcaggc cgcggcccac gccgcagacg gctacgcgag
    10921 ggccacgggg cgcgtggggg tgtgcgtggc cacgagcggc ccgggtgcga cgaacctcgt
    10981 taccggcctt gccacggcac agatggactc tgtgccgctg gtggccataa cgggccaggt
    11041 tgggcgccct gcgctgggca aggattcgtt ccaggagacg aacgtgatgg gcatgacgct
    11101 gcccttcacc aaacactctt tcctggtcga ggaccccgac gagctgtacc ccacggtgag
    11161 gcgggcgttc gagatagcac gctcaggcag gcccggaccg gtcctcatcg acgttccgaa
    11221 agacgtgcag ttcgccgcgt gcaacgataa cgggtacagg cgcctcaggg aggcccatcc
    11281 gcccgttgag tcgcctgggc tggagcgggc tgcgctgctc ctcaggaacg ccaggcggcc
    11341 cctgatcctg gccgggcacg gcgtcacgct gggcggcgcg gagaaggagc tgaggctgct
    11401 cgccgagagg accggcatcc cggtgatcac cacgcttctg ggcataagcg cctttcccga
    11461 cgaccacccg ctctacctcg gctggcccgg catgcacggg caggcatcgg tgaaccgtgc
    11521 catcgaccgc tccgacgtgc tgttcgccgt ggggatgcgc ttcgacgaca ggatcaccgg
    11581 cgcggtccac aagttcgcgc ccaacgcgaa ggtgatccac atcgacatcg acccagccga
    11641 gttgggcaag gtcgtaaagc cgaccgtcgg catcgccgcc gacgcgaagg ccgccatgcg
    11701 caccatcctg ggccacctcg aggagggccc ggagggcgaa ggatgccggc cgtggcgcga
    11761 ggagctcgag aagagccagg agccggagcg tcggcgcaaa gagtcggcgc acaccgagta
    11821 cggcccgata caggtgatgg aggccataaa ggaggcggcc ggcccgaacg tgcggctcgt
    11881 cacggacgtg ggccagcacc agatgtgggc cgcccagttc ttcgagttca ccagggcgaa
    11941 cagccttatc accagcggcg gcctcggcac gatgggcttc gccctgccgg cggccatggg
    12001 cgtggccttc gcctatccgg acgagcccgt ctgggtgatc gccggcgacg gcggcatcca
    12061 gatgaacatc caggagctcg ccacgatcag ggagttcggc ctgaacatca aggtggccgt
    12121 gctgaacaac ggctacctcg ggatggtccg ccagtggcag cagttcttcc acaaccgccg
    12181 ctactcggag acgcccataa cgggcccgaa ctacgagcag ctcaccgccg cctacggcct
    12241 ctcggggcgc accatcagcg agggcgacga cgtgggggag gcggtcaggt gggcgcagga
    12301 gcaggaaggt tgcaccttcc tggacttcca catcgacccg gaggcaaacg tgtaccccat
    12361 gatcccctcc ggcatgtcgg tggccgagat gatcgaggaa gacggtgcct gagtcggcga
    12421 ccggggtctc cctgaacacg gtcgaggtcc gcttttccgg agggatgctc gccctgaaca
    12481 ggctcctgat gacgctccag aacaagcgca tgcccgtggc gaacttcaac ctgggcaacg
    12541 acccgcaagg catgcgggcg accgtcctgc tcgactgtcc gcccgagtcg gcgctccgtt
    12601 acacggccat catctccgcg ctcgaagacg taacagaggc gggcccggcc cagaccatag
    12661 aggtcgccct gatcgagacc tcggcagact ggcgggcgat cgcggagagg tctggggtag
    12721 aggcgcacga gaacgaggga accctcgtgg cgacgggaga gccggagaac gtcgaaggct
    12781 ttctcgcggc gcttggtgat gacgtagatg atttggtcag gatgggcccc gtggcccgtc
    12841 ccgacgctcg gggaggggtc tagatggcgc gggtataccg tgaaggagat attggagacg
    12901 agatcacgac ccgcaaggtc gcgatcctgg gctacggaag ccagggccac gcccacgccc
    12961 tgaacttgaa ggagtcgggc tgcgacgtga ccgtcgggct ctacgagggc tcgaagagct
    13021 ggtcaaaggc cgaggaggac ggcctcccgg tcaagaccat tgcagacgcg gcgagagatg
    13081 ccgacgtggt gatgatgctt ctgcccgacg agaagcagcc tggcgtgttc gccaccgagg
    13141 tcgggccgaa cctcaccgag ggcgacctca tgctcttcgc ccacggcttc aacatccact
    13201 tcaaccagat cgtggtcccc ccagaggtgg acctcgggct cgtggccccc aaggggccgg
    13261 ggcacgtgct caggaggctc tacgtggagg gaaagggcat gccctccctc ttcgccgtgg
    13321 agaacgacgc ctccggccag gccagggaca ccatactctc ctacgcccgt ggcatcggga
    13381 gcggccgggc cggcatcatc gagaccacct tcgccgagga gaccgagacc gacctcttcg
    13441 gggagcaggc ggtgctgtgc ggcggcctga cggcgctgct caaggccggg ttcgatacgc
    13501 tggttgaggc cgggtaccag ccggagctcg cctactacga gtgcgtgaac gagctgaagc
    13561 taatagtcga ccttatctac gagggtgggt tggccgggat gcggtactcc atctcgaaca
    13621 ccgccgagta cggcgattac acggccgggc cgaagatcat cgacgaggct gccaaagaga
    13681 ggatgcgggt catcctctcg gacatccaga gcggcaagtg ggccaaggac tgggtgctcg
    13741 agaaccaggc cggcggatcg agcttcctcg caatgcgccg cagggaggct gaatctcagg
    13801 tcgagaaggt cgggcaggag ttgcgggcgc tcgcggccga gggcatagag accccggcgg
    13861 ggcgagcagg aggtgagaac ctatgagtcg taaagcattc gcggcgtcgg acgccgagtg
    13921 ggcctaccac gggggcgagc tggtcaggct gggagatgta cgcctctccc ccgccaccca
    13981 cgccctgaac tacggcacgg gcgtcttcga gggcatcagg gcctactgga gcgaggagcg
    14041 tggcacgctg caggtgctca agctgcgcga gcactacgag cgcttcgaga agtcctgccg
    14101 cctgctgcgc atagacctgc ccatgagcgt ggacgagctc tgcgacgcca ccctggagat
    14161 cctgcgccgc aatgccccgc gtgtggacac ctatataagg ccgcttgcct tcaaggccgt
    14221 ggagtcggtc ggggtgaacc tctcggggga gtcggagctc tccatcttca cggtccccat
    14281 gggcaactac gtggagctca ccggcctcaa ggtctgcgtc tcctcctggc ggcgcacgcc
    14341 ggacaacgcc atcccggcgc gggccaagtg cacgggctcc tacgtcaata cggccctcgc
    14401 ggtcgacgag gcccacaggg gcggctacga cgacgccatc ttcctgaccc aggatgggca
    14461 ggtctcggag gcgagcgccg ccaacatctt cctcgtgagg aagggcgtgc tcatcacgcc
    14521 gccggttacg gcggacatcc tggagggcat aacccgcgac gccgtgatgg agctcgcgga
    14581 gaaagagctt gggatgcccg tagtggagcg cgacgtggac aggacggagc tctacgccgc
    14641 cgatgaggtg ttcctctccg ggacggggtt ccagatcgca cccgtcgtcg aggtggacga
    14701 caggcccgtc gggacgggca gcacgggtcc cgtcgccgag cgtctgcagg agctgtactt
    14761 caaggccgcg cgcggcggtt gggacgagta cgcccactgg accgtgaccg tcgaggttgc
    14821 ggagccggcg aggcagtgag atactgccac ccttcatgaa ggtctacacc taacatcgtg
    14881 cctctctcct ccgctctacg actaaccagg gtggcctgcg ggcgaattac ccgtcccggt
    14941 ctctagccgg gctgcccgcg gccaccacac accaggccgc acttcaagct tcgttttcga
    15001 tgtagagagg atgagacaga tgcgtcgagt agagatcttc gacaccaccc tccgcgacgg
    15061 tgaacagtcg cccggcatct ccctgtcggt ggaggagaaa gtagagatag cccgccagct
    15121 cgcccgcctg agggtcgatg tgatagaggc gggctttcca attacgagcg agggggattt
    15181 cgagtcggtc tcccgcatcg ccgccgaggt ggctgggccg aggatcaccg gcctcgcccg
    15241 cattcacgag gaggacatcg tcagggcgtg ggaggccgtg cagtggtcgg ggaggccgcg
    15301 catccacacc ttcgttggga ccagcgatct gcacatagag caccagatgc gctcgaacag
    15361 gaacgacatc ctgcagcggg ccggtgaggc cgtggccctt gccaagagct tatgtgagaa
    15421 cgttgagttc tcacccatgg acgcgacccg cacggatata gggtatctct ccgaggtcgt
    15481 cgcggtggcc gtggaggccg gggccgacgt gatcaacatc gccgacaccg tgggctacac
    15541 gacgccggtg gagttcacgg ccttcctgaa ggagcttcag gcgcgggtgc cagggctctc
    15601 tgaccgggtc ctctcggtcc actgccacga cgacctgggg atggccgtcg ccaactcgct
    15661 cgccggggtg gagatcgggg ccggccaggt cgaggtcgcc atcaacggca tcggggagag
    15721 ggccggtaac gcctcccttg aggaggtcgt gatggcgctc gccacgcgct ccgatttcta
    15781 cgacgtcgag gtcggggtgg agaagcgcca gctggccaac acgagccgca tgatctccaa
    15841 cattacgggc tacgatgtcc ccccaaacaa ggctatcgtg gggcgtaacg ccttcctgca
    15901 cgagtcgggc atccaccagg acggtgtgct aaaggaccgc cgcaccttcg agatcatgac
    15961 caaggaagac atcggcctgg aaggcacgaa catctttctc ggcaagcact cgggccgtca
    16021 cgccctgaag gacgccctgg aggagcttgg ctactacgtg gaaggcgagg tgctcaagcg
    16081 ggccttcatc cgcttcaagg agatagccga ccacaagaag accgtaaccg cagccgacct
    16141 cgaggccata gcggccgatg aggtgggctc cttcgagggc aagttcgtgc tcgagtcttt
    16201 ccgggtggta gccgcaacgg gccgccagag cagggccgcc gtgaccatct cgcacgccga
    16261 gcagggcacg ttcgaggccg aggccgaggg cgagggcccc gtcgacgcgg tgttccgcgc
    16321 catcgacgcc gccacgaaca tcaagggcag gctaacggac ttccgcatcg acgccgtaac
    16381 aggcggcaag gacgccctcg gcgaggtcag gatctccgtg gagttcgagg ggaacgacta
    16441 cgccggacgc ggcctctccc aggatgttgt cgaggcggcg gcgcgcgcct acgtgcgggc
    16501 cgtcaacgtc tacacggcgg gcatggtcgg agccaggtgg gacgcgcggg tagcgccgta
    16561 gagatcccgg agagaaggga tagcgccccg ccgggttacg agagcctgga tgggaacatt
    16621 cccgtcctcc acgccccgcg cctggaggtg aagtcccggg agatcgggga gtacctacag
    16681 gagggctaca gggccctctc ccgggtactc tccgcaggga cgccggagat cgccgcggtc
    16741 ctggtggcgg acgaggactg gcgggatgcg ccgcgagata acgcgcttcc ctacccgccc
    16801 ggcctcccct acttcacccg ctcggtagaa ccgccgcttc tggtgctgcc cgagtccctg
    16861 tcgcccgtgt tcgggccgaa gacggaagcg atgctgccgc tcatcgtctg gcacgagctg
    16921 gcccacgctt ttctgctctc gcgggaggtc gtgaggaccc cggtctggct cggggagttc
    16981 gtcccgcagg ccgcctcggt gaccgtcgcg agaagggccg gcctgccgct gggcgcccac
    17041 ctgtcgcaga tagagagaga gccagggttt acggtgagga gcttccgtgg cccgggtggg
    17101 ccggaagacc agatgtcgtt ccagaacctg cttctggttc tggcggaggc ggcgctcgat
    17161 gccttcggag aggccttgct ggaacgcctc ttctacggtc tctgggagca agacgagata
    17221 gtcgacgaag agcgggccga ggagttgctc gcggaggccc tcggcccagg tgggcgggag
    17281 tggctcgcgt cgaggcccga gttctaggag gatacgcttt gcgagactca ttcgagatac
    17341 tactgctacc cggggacggc ataggaccgg aggtggtcgg ggcagcccgc gaggtaatag
    17401 acgccgcggc cgaccacttc gacctggaga taagctacac ggaacgcagg atcggcggca
    17461 tcgccctccg cgaggaggga gaggccgtct ccggggagac ccttgatgcg gccctcgcct
    17521 ccgactcggt cctgctcggg gcggtcggcc accccgactt cgacgacgcc ccggtacgcc
    17581 cagaagcggg cctgctcggc cttagaaagc acctcggggc cttcgccaac ctcaggcccg
    17641 tcgaggcaat accggcgctg ctcgcgtcgt cccccctcaa gcccgaggtg gtcgagggcg
    17701 tggacgtgct gatcgtgagg gagctgacgg gtggcaccta cttcggggag aaagaggagg
    17761 gcgaggaccg ggcgagtgac ctgtgcctgt acacgaggga ggagatagag cgggtggcgc
    17821 gggtcgcttt cgacgccgcc acccggcgcc ggcggcgggt tacaagcgtg gacaaggcca
    17881 acgtcatggc caccagccgg ctgtggcgca gggtggtcac cgaggtaggc cgagactacc
    17941 ccagggtgga gctcgaccac atcctggttg acgccgcggc catgtatctc gtccagtccc
    18001 cggggaggtt cgacgttatg ttgaccgaga acctcttcgg cgacatcctc tccgacgagg
    18061 cggccatgct cccgggctcg atggggatgc tcccgagcgc gtcgttgggc gaaccaggct
    18121 ctcccggcat cttcgagccc gtgcacggct cggccccaga tatcgcgggg gagggtaagg
    18181 ccaaccccta cgcaaccatc ctgtcagctg ccatgatgtt caggcacacc cttggccggc
    18241 ccgacgtggc cgaggccatc gagcagggcg tctccgtggc cctggaggcc cactatctaa
    18301 cccccgacct cggcggcaag aagacgaccg aagaggtcac gaaggccgtg agccagtggg
    18361 tcggggcagg ggagggcgtg atatgagcat agagcttttc gatacgaccc tacgcgacgg
    18421 gacccagcgc gagggggtga ccctgactgc cgaggacaag gcccgcatag cctacgagct
    18481 cgactctctg ggcatccact acattgaggc gggcttcccg gcctcaaacc caaaggacct
    18541 ggagttcttc gagagcttcg acgccgcctc gctctcgcag gcgaggctcg tcgccttcac
    18601 gcgggcgcgg cggcccggcg ggagagccga ggacgacccc gccgtcacgc tactgccctc
    18661 cctctcggcg cccgtcgcct gcatcgtcgc caagggctgg aagcttcatg tcgagaaggt
    18721 cctcaggacc acgccggagg agaacctgga gtccgtcagg gacaccgtct cctacctcgc
    18781 cgacgccggc aaagaggtca tcttcgacgc cgagcactac ttcgacggct ttcgcgacga
    18841 cccggcgtac tcgctgacgc tgcttcgggc cgccgccgag gccggggcca ccacgcttgt
    18901 cctctgcgac accaacggcg gcaccctccc gaccgagttg aaggcgatcg tggcgcggac
    18961 cgtgcgcgag ttccccgacg tcagggtagg catacacacg cacaacgacg cggggtgcgg
    19021 ggtggccaac gccctggccg cggtcgaggc cggggcgacg cacgtgcagg ggacggtaaa
    19081 cggcgtgggc gaacggtgcg gcaactgcga cctcgtcacg accatcgcca atctgcagct
    19141 caagatgggc ctcaagatga tggaagagga ccaattgcgg cgtctgacgg agacctccaa
    19201 ctacgtctcc gagctaatga acctcacccc ggacacacac agaccctacg tcggacgcag
    19261 cgccttcgcc cacaagggcg ggctgcacgt cgacggcatc tcaaaagacc cagcaaccta
    19321 cgagcacgtc ggacccgagg tcgttggaaa cgtgcgccgc acccccgtgg gcgagctctc
    19381 gggcaagaac tccataaggg ccaaggccga ggagcttggg ctggagccgg gggacgcgac
    19441 ggaggtgctc ggggcgatca agcagcgcga gtacgagggg taccactacg aggccgccga
    19501 cgcgtcgctg gccctgctca tcggacggac cacgggagat gacacgccgc tcttcgaact
    19561 ggagacgttc aggatcatca ccgagaagcg cgccggaggt cataccacga cggaggcgac
    19621 cataaagctc tccgtggacg gccacagggt gatctcgacc gccgagggca acggacccgt
    19681 aaacgccctt gacggagcac tgagagaggc catcgggccc cactatcccg agctcaagga
    19741 gatacacctc tccaactaca aggtccgtat cctggacgag caccgcgcca ccgccgccac
    19801 gacccgcgtc ctcatagact cgacagacgg caa
//
LOCUS       DBNGVD010000166         2413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_945460, whole genome shotgun sequence.
ACCESSION   DBNGVD010000166 DBNGVD010000000
VERSION     DBNGVD010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_945460"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC16CM_04685"
     CDS             complement(<1..612)
                     /locus_tag="AC16CM_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3139156.1"
                     /translation="MTSGRNFEAEQNSAREEEASGSAATTEQVATTVGPKDGDDTNVA
                     KVARGAGISTAGQGVGKLLAFATQPVLARLLGPTFYGFYAAGVALINGLQIVSRFGME
                     NGVVRYVAHYREHEDAARVRGTIILALLFPVALSLVFSITLFFGAGCRASSGWLEENG
                     TMMATVLRGFAFVLPFFVFMSMTAWATQGFKTVTYAAYIQQLIR"
     gene            complement(806..2146)
                     /locus_tag="AC16CM_04690"
     CDS             complement(806..2146)
                     /locus_tag="AC16CM_04690"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3139157.1"
                     /translation="MNAILIILDSLRKDHIGAYGNEEIQTPNFDALAGESLRFTRALP
                     DSLPTLCARRAIHTGMRTWPFRNFYRLPGETFQPAGWQPIPETQTTLAEIAAGAGYNT
                     AIFADTQPMFHPSMNFQRGFKVFEWLRGQERDRYRPKLGIPEHELRQNTVPGNDENMV
                     DKVRQYLANTRGRKSEKDYFAPRLFRRGMEFLDTADGGLPFLLVLDSFDPHEPWDPPE
                     EYVKLYSDDYNGRDPIVPNYGSSDWITDAEMKRMKALYAGEVTMVDRWLGHFLDKMEE
                     TGRMEDTFLVVVSDHGVALGEHGFTGKPYNALWPELTDIPFFIRSPDGKGAGEASDYR
                     ASTHDVASTIVGALGLLPPQPMDGQYLTLLPGDSGPEPRDHFTLGYNQYLTTRDDRYA
                     LISVNDRSEAKLYDIQKDPDMHDDIAADNQDIIDKMFDDYVLKDAGGPLPPVPS"
BASE COUNT          385 a          739 c          815 g          474 t
ORIGIN      
        1 tcgtatgagc tgctggatgt aggccgcgta ggtcacggtc ttgaaaccct gcgtagccca
       61 ggccgtcatg ctcataaaca cgaagaaggg caacacgaac gcgaaccccc gcaacacggt
      121 cgccatcatg gtcccgttct cctcgagcca accggagctg gcccgacagc cggccccgaa
      181 gaagagcgtg atagagaaca cgaggctcag cgctacgggg aagagcaacg ccaggattat
      241 ggtgccgcgc accctggcgg catcctcgtg ttcgcggtag tgggcgacgt agcgcaccac
      301 gccgttctcc atgccgaacc tggagacgat ctgcagcccg ttgatcagtg caacgccagc
      361 ggcgtagaag ccgtagaacg tggggccgag cagccgcgcg agtacgggct gggtcgcgaa
      421 cgcgaggagc ttgccgacgc cctgtccggc cgtgcttatg cccgcgccgc gcgcgacttt
      481 ggcgacgttg gtgtcgtctc cgtcctttgg cccgaccgtg gtcgccacct gctcggtggt
      541 agcggcgctg ccggatgcct cctcctcgcg tgcgctgttc tgttccgcct cgaaatttcg
      601 tcctgaagtc acggcgacag attatagtga ttttgagcca aacggaaagc ccctctcata
      661 ccaagccttt ttgtgatgct acgctgccgc gcgagtacca cgcttcgtgc gggccgtgga
      721 ttcccgcttt cgcgggaatg acgggtgggg aaggcccggt gcgtgcccgt gtgcttcggg
      781 cacccccata gaggctgacc cggtatcatg agggaacggg gggcagcggg cccccggcgt
      841 cttttagaac gtagtcgtcg aacatcttgt cgatgatgtc ctggttgtcc gcggcgatat
      901 cgtcgtgcat gtccgggtct ttctgtatgt cgtagagctt ggcttcggag cggtcgttca
      961 cgctgatcag ggcgtaccgg tcatcgcggg tcgtgaggta ctggttgtag ccgagcgtga
     1021 agtggtcccg cggttccgga ccggaatctc ctggcaggag ggtcaggtac tgcccgtcca
     1081 tcggctgggg cgggagcagg ccgagggcgc cgactatagt ggaggcgacg tcgtgggtgg
     1141 aggcgcggta gtcgctggcc tccccggccc ccttgccgtc gggggagcgg ataaagaacg
     1201 ggatgtcggt tagctcgggc cagagcgcgt tgtacggctt gccggtgaag ccgtgctcgc
     1261 cgagggccac gccgtggtcg gagaccacca ccaggaaggt gtcctccatg cggcccgtct
     1321 cctccatctt gtccaggaag tggccgagcc agcggtcgac catcgttacc tcgccggcgt
     1381 agagcgcctt catccgcttc atctcggcgt ccgtgatcca gtcagacgag ccgtagttcg
     1441 ggacgatggg gtccctgcca ttgtagtcgt cgctgtagag cttgacgtac tcctccggcg
     1501 ggtcccacgg ctcgtgcggg tcgaagctat cgagcacgag gaggaagggc agccccccat
     1561 ccgcggtgtc gaggaactcc atcccacgcc tgaagagcct gggggcgaag tagtctttct
     1621 cgctcttgcg gcccctggtg ttggccaggt actggcgcac cttgtcgacc atgttctcgt
     1681 cgtttcccgg gacggtgttc tggcgcaact cgtgctcggg aatgcccagt ttggggcggt
     1741 agcggtcgcg ttcctggccc ctcaaccact cgaacacctt gaagccgcgc tggaagttca
     1801 tcgaggggtg gaacatgggc tgggtgtccg cgaagatggc cgtgttgtag ccggccccgg
     1861 ccgcgatctc ggcgagcgtg gtctgcgtct ccgggatggg ctgccagcca gccggctgga
     1921 acgtctcgcc cggcagcctg tagaagttcc tgaagggcca ggtcctcatg cccgtgtgta
     1981 tggccctccg cgcgcagagg gtcggcaacg agtctggcag ggcccgggta aaacggaggc
     2041 tctcaccggc gagggcgtcg aagttggggg tctggatctc ctcgtttccg taggcgccga
     2101 tgtgatcctt cctcaagctg tccagaatga ttagtatggc gttcaccgtt cgtacccccg
     2161 tataagagtc gagcaacccg gacctgcggc cgggacgccg ctattctaca gcgcttttgg
     2221 gaaagatagg ctacaggcta caggtttcag gcttcaggga gatcacctgt agccttctgg
     2281 cgggcgaacc aggccagcgt tctctccagg ccttcgcgga ccggggttcg cggctcccag
     2341 cccagcgact cgcgcgccct gatcctgggg ccgcggtcgt ctctcaccgt gacggtgagc
     2401 caggtatgga gcc
//
LOCUS       DBNGVD010000167         8724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4770445, whole genome shotgun sequence.
ACCESSION   DBNGVD010000167 DBNGVD010000000
VERSION     DBNGVD010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4770445"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="AC16CM_04695"
     CDS             complement(<1..1205)
                     /locus_tag="AC16CM_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3139158.1"
                     /translation="MSVNGFRTFASLLKSHRGAAELTQEELAGRAGLGTRTISNLERG
                     INKAPYRATIRRLADALGLSDEDLTRFAASARRPPKHPSDGSRMAVEGGFLGALPAAH
                     LVAREQELERVLRALEAAREGLGRLVLLTGEPGIGKTRLAQEAGVRAWEGGFIVASGR
                     CYEAQSGVPFYPFLEALSTLYEDAPQRVRGHVPERWPYLARLLPDHFPLAASGSSEEA
                     QRLLRSVTGFVRELSAERPVVLLLDDLHHADGASVDLLAHLARYTRTDRVLLVGTYRE
                     AEAGPDLGRAARELDREQLVEKVRVRRLGREETAELMSDRLDGAEVPEQFSGRVFDRT
                     GGNPFFTVEILKSLIERGDLFLREGRWFRKDMQDLAAPESVSEAILERVSRLNPRTRQ
                     TLEKASVLG"
     regulatory      1586..1666
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1779..3143
                     /locus_tag="AC16CM_04700"
     CDS             1779..3143
                     /locus_tag="AC16CM_04700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018796615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3139159.1"
                     /translation="MQDELAIGGTELTGRLVFPDSPDYERARLGWARQFSRFPLVIVF
                     CQNAQDVVNAISWCRENDVAFRARSGRHALEGWSSLDGGVIIDVSEMNGIDVDEDAGV
                     ATVQTGATQGAVVEALGKLGYTIPSGGEGTPGIGGVTLGGGIGWLSRSMGLVCDHLVG
                     LEMVIPSGEQGASVVQADESQNADLLWASRGGGGGNFGIATSYTFRLVPISTVAFYEV
                     TWGEQDWQLLDELLGVWQELAPSAPDELGSVFFASSKAEGTITTYGVLDGSEEQLRQM
                     LQPLLEVGEPEVTIETLPYLDAWLRIAATPEDDPPRKDKFSSVWVYESLPPEGIGTVR
                     RFLEDAPNPTADIWCLSWGGEVSRTPTDATAFYHRKPRYYIEWDISWEEAEEGSEVFA
                     WTEQFRLAMAPYAEGSYINVPDSSINDLGTYYGDNLARLREVKSKYDPENVFHFEQSI
                     PPAG"
     gene            3224..4627
                     /locus_tag="AC16CM_04705"
     CDS             3224..4627
                     /locus_tag="AC16CM_04705"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139160.1"
                     /translation="MIEDRDPTKDASSAGQEPASENGAPSKKVSRLAVWSYVLFNFGD
                     TPFAATILTLYFPLWLTEEYRAGPALFNYATALASLLVVLSAPALGAICDLRQSRKPS
                     LVVFTLITVFCTFSLAFSDELTGSLSVAIVLFVVAVVAFNLINVPYYALLRSASEGRG
                     TGKISGYTQAAGFIGTFIAVIGLTLLVAPEKFLGFTIGGPGEIRQVFGPLGAFVHTAK
                     ATVDSNTFLPTAVFFLVFALPAFFFVADVPERAPQRLRLGQAYRNVFATVKGIGAYTG
                     LGSYMVVTLLYMEAALIAIPNMCLFGKDVFAMKDQQISNLIIFSLLFSVVAAFGAGRV
                     SDKIGPKRALLATMGLWVVGILAVTFAWAPWVMYVASPLIFLANGATLAVGTVLVIAL
                     SPPSKLTEFMGLYVTVGMVSAVLGPAIVGLLLGVFGGLDAGAYRIGMGSIAVTMVIGI
                     FLLIARVPDARNTDAPS"
     gene            4712..5020
                     /locus_tag="AC16CM_04710"
     CDS             4712..5020
                     /locus_tag="AC16CM_04710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3139161.1"
                     /translation="MHARVSILEGDPDQIDHGLRYFREEVQSTLEAQPGFKGAVSLHD
                     VQNRHNGRTISITFWESEDHMHDNGGDHSRVLSKVAQTEGRELVGVEWYDVGVFELNV
                     "
     gene            5164..5517
                     /locus_tag="AC16CM_04715"
     CDS             5164..5517
                     /locus_tag="AC16CM_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139162.1"
                     /translation="MSEGSARTEMERRLIERSLEDDAFRQKLVEEPKGVVEQELGMRL
                     PEEVRVMVVEETSDTIYLVIPGTPMAGREELELSDQELESVAGGGSWGQDTCQSTCGG
                     DCTPNCTGDFICTTG"
     gene            complement(5616..6128)
                     /locus_tag="AC16CM_04720"
     CDS             complement(5616..6128)
                     /locus_tag="AC16CM_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity protein"
                     /protein_id="HIR3139163.1"
                     /translation="MIFGKQRDPRLVTVRRGGTLRDVDHHRLAFWAADCAEHVLHHFE
                     QAHPGDDRPRHAIELARAWARGEITMTQARGAAFGNAAAREASGAAKQAALAAAQAVA
                     VSHVPAHELGAAAYAIRASRAAAPDGEREQAGRRECRWQRAQLPGEIRELVLDDQRLR
                     NEVCWYVFDC"
     gene            complement(6290..7282)
                     /locus_tag="AC16CM_04725"
     CDS             complement(6290..7282)
                     /locus_tag="AC16CM_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3139164.1"
                     /translation="MEEVYAALARVPLFGNVRREGIKIERLEGGLTNVCYKVTVGNSA
                     YVLRLAGEGTSGYIDRSSEEHNAMVAAAAGVGAEVVYFDARDGMMLTRFVEGASVNHR
                     GGFGPGAPARAARTLARVHGLGDVFNSRFDVFAAMARYLELLREQGIRLPEGYGESRR
                     CARAVRLALEASPAPLVPCHNDPWPGNLLDAGGSVYLIDWEYSGMNDAMWDLAGLSVE
                     AGFGPWQDREMMEAYYDQIPSRALYSRLEVYKAMGDLHWSLWSFVRHKKDDPARDLWG
                     YGLERLARANARMGSAGFGQHLDLVRGSPSLVSQPGERSPLSGHIRPAYPRSSS"
     gene            complement(7421..8251)
                     /locus_tag="AC16CM_04730"
     CDS             complement(7421..8251)
                     /locus_tag="AC16CM_04730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139165.1"
                     /translation="MSSYEDYTRTSGNYDRTREPVGTEILVGCFAHAPVPLAQAVVLD
                     AGCGTGSYSDALLHYVGRIEAVDLNPGMLEVASRKLSWAGDRVAFHPARIDELPFGDA
                     TLDGVMINQVLHHLPDDASKGFPAHRGAFREFARVLKPGGVLTVSTCSQEQLRAGYWH
                     YHLIPEAADALRSRYIPLDELVEILDDSGLEHRGSFVPTDATVQGKAYFDPHGPLNQE
                     WRDGDSVWSLVGEARLERALSRIRRLDERGELGTYVARNDALRPHIGQATVVFASRAG
                     "
     gene            8433..>8724
                     /locus_tag="AC16CM_04735"
     CDS             8433..>8724
                     /locus_tag="AC16CM_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139166.1"
                     /translation="MIFGSVGTNLSGLYVDGVRKLGEDLEVLEGSGADFVEVWPQNLG
                     VILGGRLDAPRLRAVGEVLLETELTYTVHAPLEVNLMDMTSGGLQRDVLDAAL"
BASE COUNT         1526 a         2774 c         2846 g         1578 t
ORIGIN      
        1 tgtccgagta cgctggcctt ttcgagggtt tgcctggttc tggggttcag acgcgatacc
       61 ctttccagga tcgcctcgct cacgctctcg ggcgccgcga ggtcttgcat atccttgcgg
      121 aaccaacgcc cctcccggag gaagaggtcg ccgcgctcga tgagtgattt caagatctcg
      181 accgtgaaga aggggttgcc accggtgcga tcgaaaaccc gccccgaaaa ctgttcgggt
      241 acttcggccc catctaacct gtcgctcata agctcggcgg tctcttctcg gcccaacctt
      301 cgtaccctga ccttttcgac gagctgctcg cggtccagtt cgcgcgcggc cctaccaagg
      361 tccggtccag cttccgcttc gcggtaggta cccacaagta gaacgcggtc cgtgcgtgta
      421 tacctggcca ggtgcgccag gaggtccacg ctcgcgccgt cggcgtggtg cagatcgtcg
      481 agcaagagaa ccacgggcct ctcggcggag agctcgcgca cgaagccggt caccgacctc
      541 agaagccgtt gcgcctcctc gctgctgcct gatgccgcca gggggaagtg atccggcaac
      601 agccgggcca gatacggcca gcgttcgggg acatgcccgc ggaccctctg cggcgcatcc
      661 tcgtacagcg tggagagcgc ctccagaaaa gggtagaagg ggacgccgct ctgcgcctca
      721 tagcagcgtc cgctcgccac tatgaagccc ccctcccagg cccgaacgcc ggcctcctgg
      781 gccagccgcg tcttcccaat acccggctcc ccggtcagga gcaccaggcg gcccaggcct
      841 tcccgggccg cctccagggc acgcaggacc cgttcgagct cttgctcccg ggccaccaga
      901 tgcgccgccg gcaacgcgcc gaggaagccg ccttcgaccg ccatccggct gccgtcagaa
      961 gggtgtttcg gggggcggcg ggccgaggcc gcgaaccggg tgagatcctc gtcggagagc
     1021 cccagggcgt cggcgaggcg gcggatggtg gcgcgatatg gtgccttgtt gataccgcgc
     1081 tcgaggttgc tgatcgtgcg cgtccccagc ccggcccgcc cggccaactc ttcctgggta
     1141 agctcggcag caccccggtg gctcttgagc aaggatgcga acgtgcggaa cccgtttacc
     1201 gacatggaaa gttcacctcg cccgcattgt gcatcccgcg acgctccggg gcaagcctgt
     1261 cccgttatca agtcggtggg taagtcggtg ggtagaaatc cttactatgg cctggtaacc
     1321 agggtaaatt gcaacccgtc ggggatgcac acggacggga ggtagttcat ctaggcgcat
     1381 catctctgtt cgccccggtg gtgaccggcg tgggcgcgga gctttcggag ccaggacacg
     1441 atagatgaca gagctgccct tcgagattcc gggagtcctc taccccagaa caacttcagg
     1501 caacggcaaa gatagctcat ttaggggatg cgcactccga tcatcagcaa ttaacctgtg
     1561 ctcgttggag gcgagcgctc gagtgcagcg ataagggcaa acccgtcgtg aggcgggggc
     1621 gcaaaaccta agggcctgct agagagcagg cagccgggtt gccgaacggc agggggtctc
     1681 gcactggact cctggcgaac cgttcctcac cggtgagagc ggagggagag gcatgctcac
     1741 aagcgtgaca agcaagcgga caggggagga cgccacacat gcaggatgaa ctcgctatcg
     1801 gtgggaccga gctcacggga cgtctggtct ttccagatag cccggactac gagagggctc
     1861 gcctgggttg ggccagacaa ttctccaggt ttcccctcgt aatcgtcttc tgtcagaacg
     1921 cccaggacgt cgtcaacgcc atcagctggt gccgagagaa cgacgtagcc tttcgcgcca
     1981 ggagcggtag gcatgccctg gaaggctggt cctccctgga tggcggggta atcatcgacg
     2041 tgagcgagat gaacgggata gacgtcgacg aggacgcggg ggtggccacc gtgcagaccg
     2101 gcgccaccca gggtgctgtg gtggaggcac ttggaaagct ggggtacacc atcccgagcg
     2161 ggggggaggg tacccccggt atcggtggcg ttaccctggg cggtgggatc ggctggctct
     2221 cccgcagcat gggtcttgtg tgtgaccacc tggttggcct ggagatggtg atcccatcgg
     2281 gcgagcaggg tgccagcgtg gtccaggccg acgagagcca gaacgccgac ctcctgtggg
     2341 cctcacgcgg gggcgggggc ggcaacttcg gcatcgccac ctcctacacg ttcaggctcg
     2401 tcccgatctc gaccgtcgcc ttctacgagg tgacctgggg ggagcaggat tggcaactcc
     2461 tggatgaact ccttggcgtg tggcaggagc ttgcgccctc cgcgcctgat gaactcggct
     2521 cggtcttctt cgcttcgtcg aaggcggagg gcaccatcac cacctacggc gtcctggacg
     2581 gatcggagga acagctgcgc cagatgctcc aaccgctgct cgaggtcggc gagcccgagg
     2641 ttacgatcga gaccctgccc tacctcgatg cctggctccg aatcgccgcc accccggaag
     2701 acgatccacc gaggaaagac aaattctctt cggtctgggt ctacgagtcc ttaccgcccg
     2761 agggaatcgg gaccgtacgc cgattcttag aagatgcccc aaaccccacg gccgacatct
     2821 ggtgcttgag ttggggtggt gaggtaagtc gtaccccgac cgacgccacg gcgttctacc
     2881 acaggaagcc ccgctactac atcgagtggg acatctcctg ggaggaggcc gaggagggaa
     2941 gtgaggtctt cgcgtggacg gagcaattcc gtctggcgat ggccccctat gccgaagggt
     3001 cctacatcaa cgtgcccgac agcagcatca acgacctcgg gacctactac ggcgacaacc
     3061 ttgcccggct ccgggaggta aagagcaagt acgacccgga gaacgtcttc cacttcgagc
     3121 agagcatccc cccagcaggg tagggtgatg gagaacaagc agcaagacga tatcaggagc
     3181 aggctccacg ctcaacggga gccggaagac gaggagagca ccgatgatag aagatcgtga
     3241 cccgacaaag gatgcctctt ctgccggcca ggagcctgcg tcggagaacg gggctccttc
     3301 caagaaggtg tccaggctcg ccgtctggtc gtacgtcctc tttaatttcg gggacacccc
     3361 cttcgcggcc accatcctga ccctatactt cccgctctgg ctcacggagg agtacagggc
     3421 cgggcccgcc ctcttcaact acgccacggc cctggcgtct ttgttggtgg tgctgagcgc
     3481 cccggccctg ggggcgatct gcgaccttcg ccagagtcgg aaaccgtccc tcgtggtgtt
     3541 caccctgatt acggtgttct gcacgttttc gttggccttc tcggacgaac ttacggggag
     3601 cctgtcggtg gccatcgttc tcttcgtggt ggccgtcgtc gccttcaacc tgataaacgt
     3661 gccctactac gcgctcttgc gtagcgcgtc ggaggggcgc ggcaccggca agatcagcgg
     3721 ctacacccag gccgcgggct ttatcgggac cttcatcgcc gtcatcggcc ttacgctcct
     3781 ggtcgcccct gagaagttcc tggggtttac gatcggcggg cccggggaga ttcggcaggt
     3841 gttcggcccc ctgggcgcct tcgtgcatac cgcgaaggcc acagtcgatt ccaacacctt
     3901 cctgccgaca gcggtcttct ttctcgtgtt cgccctgcca gccttcttct tcgtggcgga
     3961 tgttcccgag cgtgctccgc agcggttgag gctgggtcag gcctatcgga acgtcttcgc
     4021 caccgtgaaa ggcatcggcg cctacaccgg cctcgggtcg tacatggtgg tgacgctgtt
     4081 gtacatggag gcggcgctca tcgccatccc caacatgtgt ctcttcggca aagacgtctt
     4141 cgcgatgaaa gaccagcaga taagcaacct aatcatcttc tcgctgctct tctccgtcgt
     4201 ggccgccttc ggcgccgggc gcgtctccga caagataggg cccaagaggg cgctcctggc
     4261 aacgatgggg ttgtgggtgg tcgggatact ggccgtcacg ttcgcctggg ccccctgggt
     4321 gatgtacgtc gcatcgcccc tgatctttct cgcgaacggc gcgacgctgg cggtgggtac
     4381 cgtcctggtc atcgccctct cgccgccctc gaagctcacg gaattcatgg ggctctacgt
     4441 cacggtcggc atggtctccg ccgtactcgg accagcgatc gtagggttgc ttctgggggt
     4501 gttcgggggg ctcgatgcgg gcgcgtaccg catcggtatg ggctccatag ccgtgaccat
     4561 ggtgatcggg atcttcctgc tcatcgcacg cgttccggat gcccgcaaca cggacgcccc
     4621 ctcatgagga cgaggcccct cttcgcacca atctatataa tggcggatct tgctccattc
     4681 gggagctttc ggagaaagga gtcgtgaagc gatgcacgct agggtgagca tattggaggg
     4741 agaccccgac cagatcgacc acggattgag gtatttccgg gaggaggtcc agtcaacact
     4801 agaggcacaa ccgggcttca agggggccgt ctcgcttcat gacgtccaga accgccacaa
     4861 tggaaggacg atctcgataa ccttctggga gagcgaagat cacatgcacg acaacggggg
     4921 agaccatagt cgtgttctca gcaaggtggc ccagaccgag ggccgggagc tggttggcgt
     4981 cgagtggtac gacgtgggtg tcttcgagct aaacgtctag gacggaatgc caccaggccg
     5041 gggctaccac gccccggcac tggcttggaa gtgtgtgcag cacgagattc tctccattcg
     5101 gcaccttgct accagaggcg gcacccaaca ggcggattcg atcgggaaac gaaaggggag
     5161 agcatgagtg aggggtcggc caggacggag atggagcgca ggctgataga gaggagcctc
     5221 gaggacgacg ctttcaggca aaagctcgta gaggagccga agggggtcgt ggagcaggag
     5281 ctcgggatgc gcttgcccga ggaggtacgg gtaatggtag tcgaagagac ttccgacacc
     5341 atctacctgg tgattcccgg caccccgatg gcgggccgcg aagaactgga gctttcggac
     5401 caggagctcg agtcggtggc cggcggcggc tcctggggac aggacacctg tcaatctact
     5461 tgtggtgggg attgcactcc aaactgtaca ggggatttca tctgtaccac tggatgatag
     5521 ggctcgccgg gatcccacct tctagaagct ccgggaccgt cacggacggc agcacgctct
     5581 cgcctgtcgc ccccctgatg tttcgggcca aactttcagc aatcgaacac gtaccagcac
     5641 acctcgttgc gcaacctctg atcgtccagc acgagctctc ggatctcacc gggaagttgt
     5701 gcccgttgcc accggcactc ccgacgaccg gcctgctctc gctctccatc cggggccgcc
     5761 gcgcgtgagg ccctgatcgc gtaagctgca gcacccagct cgtgggctgg cacgtgggat
     5821 accgctacag cctgggcggc ggctagggcc gcctgtttcg cggcccccga ggcctcccgg
     5881 gccgccgcat ttccgaaggc ggccccgcgg gcctgcgtca tcgtgatctc accccgcgcc
     5941 caggcgcgtg ccagttcgat ggcgtggcgt ggccggtcgt ctccgggatg cgcctgctcg
     6001 aaatggtgca gcacatgctc ggcgcaatcg gccgcccaga acgcgagacg gtgatgatct
     6061 acatccctca gggtgccgcc ccgacgcacg gtcacgagcc gagggtctcg ctgcttaccg
     6121 aagatcatct gcctgccacc cgtctctgat catacgcccc gattgtacct acgggctcac
     6181 cgtcaaaacc gagatgatcg cgcaggcttt cagcagtcag ctacctggca gaaagctcgt
     6241 taccgcccgg gagtgttaga tactctgcaa gccggcgcgg agggcggtct caggaggacg
     6301 aacgagggta ggccggtctg atgtggcccg agagaggact acgttcgccc ggttgcgata
     6361 ccagggaggg tgacccgcgc accaggtcca ggtgctggcc gaagcctgcg ctgcccatcc
     6421 gggcgttggc acgcgcaagc ctctccagcc cgtagcccca gagatccctg gccgggtcgt
     6481 ccttcttgtg ccggacaaag ctccagagag accagtggag gtcgcccatc gccttgtaga
     6541 cctcgagccg cgagtagagc gcccgagacg gtatctgatc gtagtaggct tccatcatct
     6601 cgcggtcttg ccaggggccg aacccggcct cgaccgagag gccggcgagg tcccacatcg
     6661 cgtcgttcat gcccgagtac tcccaatcta tgaggtagac gctccctccc gcgtcgagga
     6721 ggtttccggg ccacgggtcg ttgtggcagg gtacgagggg ggcgggggag gcctcaagcg
     6781 cgaggcgcac cgccctcgcg caccgccgtg actcgccgta accctcgggc aggcgtatgc
     6841 cctgctcgcg caggagttcc aggtaacggg ccatcgcggc gaaaacgtcg aagcgggagt
     6901 tgaaaacgtc cccgagaccg tggacccgcg cgagcgttcg ggcggccctt gccggtgccc
     6961 ccggtccgaa ccctccccgg tggttgacgg aggccccctc gacgaaccgg gtcagcatca
     7021 tgccatccct ggcgtcgaag tagactacct ccgcgccgac ccccgcggcg gccgcgacca
     7081 tcgcgttgtg ctcctcggag ctacggtcga tataccccga ggtgccttcc ccggcgagcc
     7141 tcaggacgta ggcggagttg cctaccgtta ccttgtaaca gacgttggtg aggccgcctt
     7201 cgaggcgctc gatcttgatt ccttcccgcc ggacgttgcc gaacaaaggc acgcgcgcca
     7261 gcgcggcgta aacctcttcc actcgtggtt ctctccctac acaaacgctt gactggtccc
     7321 caccggcgcc cgtggtgcgg tcgaatgatt gtaaaccccg tgttaaccgc atgcaatctt
     7381 ccgatcttgc ggcctccgat gatcgggggc cggcgccggc ctacccggcg cgggatgcga
     7441 agacgacggt tgcctggccg atgtgtgggc gcagcgcgtc gttgcgcgcc acataggtcc
     7501 cgagctctcc ccgttcgtcc agcctccgga tgcgggagag ggcccgctcg aggcgggctt
     7561 cgccgaccag cgaccagacc gagtccccgt cacgccactc ctggttgagg ggcccgtgag
     7621 gatcgaagta cgccttgccc tgtacggtgg cgtcggtggg cacgaagctc ccccgatgtt
     7681 cgagcccgga atcgtcgagg atctcgacca gctcgtcgag cgggatgtag cggcttctga
     7741 gggcgtcggc ggcctctggg atgaggtggt agtgccagta gccggcgcga agctgctctt
     7801 gcgagcaggt gcttaccgtg agcaccccgc ccggcttgag caccctggcg aactcgcgga
     7861 acgccccgcg gtgggccgga aagcctttcg aggcatcgtc cggcaggtgg tggaggacct
     7921 ggttgatcat gaccccgtcc agggttgcgt ccccgaaagg tagctcgtcg atcctggccg
     7981 ggtggaaggc cacccggtcc ccggcccagg acagcttgcg cgaggcgacc tcgagcatgc
     8041 cggggttcag gtcgaccgcc tctatccggc cgacgtagtg gagcagcgca tcagagtagc
     8101 tgccggtgcc gcacccggca tccagcacca ccgcctgcgc cagcgggacg ggcgcgtggg
     8161 cgaagcaacc gaccaggatc tcggtgccaa cgggctccct ggtcctgtcg tagttgccgg
     8221 atgttcgcgt gtagtcctcg tagctgctca actcggcctt tcttctaggc tggcgtcttc
     8281 ccggtacggg tctcctgcta agggtacacg gattcgctgg agatctccga ttgaacgcaa
     8341 gttttacctg ccgttaaccc gccttttacc cagggtccga ccggcgagcc tatactgcgc
     8401 ccgatcccgt atggacccga gggagggacc ttatgatctt tgggagcgtg ggtacgaacc
     8461 tgtcagggct ctacgtggac ggcgtgcgaa aactcggaga ggacctcgag gtacttgaag
     8521 ggtctggggc ggactttgtg gaggtctggc cgcagaacct gggggtgatc ctcggtggcc
     8581 gcctggacgc gccgaggctg cgggcggtcg gagaggttct gctagagacg gagctgacct
     8641 atacggtcca cgctcccctg gaggtaaacc tcatggacat gacctctggc gggctgcagc
     8701 gcgatgttct cgatgccgcc ctcg
//
LOCUS       DBNGVD010000168         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4224739, whole genome shotgun sequence.
ACCESSION   DBNGVD010000168 DBNGVD010000000
VERSION     DBNGVD010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4224739"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="AC16CM_04740"
     CDS             <1..640
                     /locus_tag="AC16CM_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139167.1"
                     /translation="VDEGEVVTLIGSNGAGKTTTLRSIHGVLPPREGKIRFAGEEIQG
                     LPAHDMISRGISQSPEGRKIFHRMTVQENLEMGAYHRNDNAGIREDIDRVFELFPRLQ
                     ERVKQEAGTMSGGEQQMLAIGRAIMSRPTLLLLDEPSMGLAPVLVERIFQTIQEINAQ
                     GTTILLVEQNANVALEIATRGYVLESGSIVNSASAEKLRQDPKVREAYLGEI"
     gene            982..1428
                     /locus_tag="AC16CM_04745"
     CDS             982..1428
                     /locus_tag="AC16CM_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139168.1"
                     /translation="MALHPGADLKESCLEISPRHPDYAARPIQTGFDWSSLSEYPFER
                     LYLVVFRSVRRPDADVDLLREHDDRAYEEALRSGGLLKYFKGQANERGECLSFCLWET
                     RESAREAAGAASHQSAAGITARMYVSYVLDRYWLKRAEGKLVFERI"
     gene            1545..>3043
                     /locus_tag="AC16CM_04750"
     CDS             1545..>3043
                     /locus_tag="AC16CM_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR3139169.1"
                     /translation="MRIYLIDGYSLLYRAFYALPQSISTSSGLPTNALYGFTSMLLKL
                     LDTEEEVGLGVVWDGGMPEFRLKIFPEYKAQRSAMPEDLRSQVDHMDEILDAMNVPAI
                     TAEGFEADDALATMSRQVPEGDELYIVTGDQDAMQLVNGDVRVLRTTRGVSETKTYGR
                     DEVIEEYGVTPEQIPDYKALTGDSSDNIPGVRGIGPKGAANLLKQFGTLDGVYENLEN
                     ISARGTRNKLEEGRENAFMSLELARMRFDVPVKLDPASLRFEGISPNIRDITRRYEFR
                     TLESRLTELPVAGGEELPPVERLEVSISDGPLGLPFEPVAASPVDDDSGRWCVAVSEK
                     EVRVTEGVPDGSLQVHDSKKLGVLDADFDTYLAAYLVKPGLGSYELETLAAERGLAEV
                     DLSHGDHAVAESARRAAQIHALSPRLRKELEEMGLARLYYDVELPLARVLGEIEDVGM
                     PVDAETLEGVGQDLDARISDVERDIYEDVGHPFNIGSPKQLGEVLFEEMG"
BASE COUNT          588 a          939 c          976 g          540 t
ORIGIN      
        1 cgtcgacgag ggagaggtcg ttacccttat aggctccaac ggcgctggca agaccaccac
       61 ccttcgctcc atccacggcg tcctgccgcc gagggagggc aagatccgct tcgcgggaga
      121 ggagatccag ggcctccccg cccacgatat gatctccagg ggcatctccc agagcccgga
      181 aggacgcaag atctttcacc gcatgacggt gcaggagaac ctggagatgg gtgcctacca
      241 caggaacgac aacgccggca tccgcgagga tatagaccgc gtcttcgagc ttttcccacg
      301 cctgcaagaa cgcgtgaaac aggaggccgg caccatgtcg gggggcgagc agcagatgct
      361 ggccatcggc cgggctatca tgagccgccc tactctcctg ctcctggacg aaccgtcgat
      421 gggcctcgca cccgtcctcg tggagcgaat cttccagacc atccaggaga tcaacgcgca
      481 aggcacgacc atcctcctcg tagaacagaa cgccaacgtg gccctcgaga tcgccacccg
      541 cggctacgtg ctcgaatccg gaagcatcgt caactccgcc tcggccgaaa agctgcgcca
      601 ggatcccaag gttagggaag cctacctcgg cgagatctag cccaaccttc tggaaaactg
      661 gctgtcagga cgagaaaagg ctgcaggttt cggccccagg aatgttgtct ggctttcttc
      721 atcggtcggc tggacaacgc ttttctgagc gcgtatagtt ggcagtgtgt taattggatt
      781 acatttccgt ttcgcgccta ctttctacgc accctcgtgg cggctcaagc gacgcgagtg
      841 gagcggctac gggttcgggc acacggcgcc aagggaacct ggcgacttgc gtggaaccgt
      901 gataggcagg aagggccggt tcaaaggtct ctggatggta ccaggaggtg acgtttagag
      961 tccatttttg aaaggtgatc catggccctc catccagggg cggacctcaa agagtcctgc
     1021 cttgaaatct cccccaggca tcctgactat gccgcccggc ctatccagac cggcttcgac
     1081 tggtcttctc tctccgaata tcctttcgag cgactttacc tggtcgtttt tcgttccgtg
     1141 cggcggcccg acgccgacgt cgacctcctg cgcgagcacg acgaccgcgc ctacgaagaa
     1201 gcgttacgtt ccggtggcct gctcaagtac ttcaagggtc aggcaaacga gaggggcgaa
     1261 tgcctctcgt tctgcctctg ggagacgcgc gaaagcgcca gggaagcggc tggcgccgca
     1321 tcccaccaat ccgcagccgg catcaccgct cggatgtatg tctcttacgt actcgaccgc
     1381 tactggctga agcgggccga agggaaactc gtcttcgaac gcatctagag cagtcagact
     1441 ccagccctcg gcgatcagct atttgctctt gctgagggct gaaagctgac agctgaaggc
     1501 tcgaggggcg ggaatccgtg ccgtcgtccg gtataatccg ggtagtgcgg atctacctta
     1561 tcgacggcta ttctctcctg tacagggcgt tctacgcgct gccgcagagc atcagtacct
     1621 cgagcggcct ccccacgaac gcgctgtacg ggtttacgag catgctgctc aagctactcg
     1681 acactgagga agaggtgggc ctgggggtcg tgtgggacgg tgggatgccc gagtttcgat
     1741 tgaagatctt ccccgagtac aaggcgcagc gctctgcgat gccggaggac ctgaggtctc
     1801 aggtcgacca catggacgag atcctggatg ccatgaacgt tcccgccatc acggccgagg
     1861 gcttcgaggc cgacgacgcc ctggccacca tgtctcgtca ggtgcccgag ggcgacgagc
     1921 tgtacatcgt aaccggcgat caggacgcca tgcagctcgt caacggcgac gtgcgggtgc
     1981 tcaggaccac ccgcggcgtc tcggagacca agacctacgg cagggacgag gtcatcgagg
     2041 agtacggtgt gacgccggag cagatccccg actacaaggc cctgacgggt gactcttcgg
     2101 ataacatccc aggcgtgcgt ggcatcggtc ccaagggcgc ggcgaacctt ctcaagcagt
     2161 tcggcacgct cgacggcgtc tacgagaacc tggagaacat ctcggccagg ggcacgcgca
     2221 acaagctcga agagggacgt gagaacgcct tcatgtcgct cgagctcgcc aggatgcgtt
     2281 tcgacgtgcc cgtcaagctc gacccggcct cgctccgctt cgagggtata tcgccgaaca
     2341 tcagggacat caccaggcgt tacgagttcc gcaccctgga gtcgcggcta accgagctgc
     2401 ccgtcgccgg tggggaagag ctaccgcccg tcgagcgcct ggaggtgagc atctccgatg
     2461 ggccgctcgg gctgccattc gagcccgtcg ccgcctcccc ggtcgacgac gacagcgggc
     2521 gctggtgcgt cgccgtctcg gagaaagagg tgcgcgttac ggagggggtg ccagacggct
     2581 ccctgcaggt gcacgactcc aaaaagctcg gggtgcttga tgcggacttc gacacctacc
     2641 tggcggctta cctggtaaag ccgggtctcg ggagctacga gctcgagacg ctcgccgccg
     2701 agagggggtt ggccgaggtg gacctgtcgc acggggatca cgccgtcgcg gagtcggcga
     2761 ggagggctgc gcagatccac gcgctctcgc cgcggctgcg aaaggagctg gaggagatgg
     2821 gtctggccag gctctactac gacgtggagc ttccgctcgc ccgcgtgctc ggggagatag
     2881 aagacgtcgg gatgcccgtg gacgccgaga ccctggaggg ggtcgggcag gacctcgacg
     2941 cccgtatctc ggatgtggag cgggatatat acgaggatgt gggccacccc ttcaacatcg
     3001 gttcgcccaa gcagctcggc gaggtccttt tcgaggagat ggg
//
LOCUS       DBNGVD010000169         3673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_763776, whole genome shotgun sequence.
ACCESSION   DBNGVD010000169 DBNGVD010000000
VERSION     DBNGVD010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_763776"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..397)
                     /locus_tag="AC16CM_04755"
     CDS             complement(125..397)
                     /locus_tag="AC16CM_04755"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004843 - cysteine-type deubiquitinase
                     activity [Evidence IEA];
                     GO_process: GO:0016579 - protein deubiquitination
                     [Evidence IEA];
                     GO_process: GO:0043161 - proteasome-mediated
                     ubiquitin-dependent protein catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-terminal hydrolase 14"
                     /protein_id="HIR3139170.1"
                     /translation="MANGACTHLDQIRDVEPSAEGCEECLQMGDRWVHLRECLICGHV
                     GCCDSSRNRHATGHFHATGHPLVRSFQPGEDWIWCYLDEVVMEPRG"
     gene            complement(424..2046)
                     /locus_tag="AC16CM_04760"
     CDS             complement(424..2046)
                     /locus_tag="AC16CM_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139171.1"
                     /translation="MSNIGNPPCDAGVISSRRADAPCAPRSGPWILAATIIGSAMAFI
                     DSTVTNVALPQIQTRLGATALDAQWIVESYALFLSALILVGGSLGDHYGRRRIFMLGV
                     AIFTLASVWCGLATSPGMLIVARAVQGVGGALLVPGSLAIISSSFEGEERGKAIGTWA
                     GLSGVTTALGPILGGFLVENVSWRAAFLINVPLAILVLVIAYKHVPESRDPDARKLDI
                     PGAALATLGLGGVVYGLITSAAAGFDNVRVIAALVLGVICIIGFVGWERRTREPMLPL
                     GMFRSRNFSGANVLTLLLYAGLGGSLYFLPFLLIQVHDYSATAAGSSFLPFTIITFLM
                     SRWAGGLIIRFGARLPLTIGPIIAAGGFMLLALPGTQGSYWTTFFPAIVVLGLGMSLV
                     IAPLTTTALSSVSGRHSGLASGVNNAVSRTAGLLAIPILGIFVFFVFSGSLDARVQQL
                     DLSPEARQQLEAEKVDLGGAEVPPGVSGDTAEEIETAVKQSFLGAFRLAMYISAGSAL
                     ASAVAAAIIIEGKNRPAPVQEADGRQEETAAA"
     gene            complement(2092..2670)
                     /locus_tag="AC16CM_04765"
     CDS             complement(2092..2670)
                     /locus_tag="AC16CM_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR3139172.1"
                     /translation="MRSRAREVLEFWFGSENDPEYGQFREEWFRKDPDFDATITARFA
                     ELYEEAAAGKLDGWREEAESCLALVIVLDQFPRNMFRGDGRTHAEDDRALSTSKYAVE
                     HALDRELPAFQRMFLYMPFMHSESVEEQRRSVEMFERLAGEPGAPDVVSYAEAHRDIV
                     ERFGRFPHRNEILNRQTTPEEAEFLTTEGSSF"
     gene            complement(2660..3247)
                     /locus_tag="AC16CM_04770"
     CDS             complement(2660..3247)
                     /locus_tag="AC16CM_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HIR3139173.1"
                     /translation="MGQTHIFEEICSEKRRVNARVLKQTVSLAVEIAREGREGRKIGT
                     LFVVGDSGEVMRRSKPLILDPLQGHADEDKQIEHHDMRETIKELAQLDGAFLVSNAGV
                     VLSAARYIDASSDNLDLPLGLGSRHMAGASISQQTSAVSVVVSESSMVRMFDDGELVS
                     EIVPELWMIEGYRSRVEGRTFTRSEEDITVIGRAE"
     gene            complement(3253..>3673)
                     /locus_tag="AC16CM_04775"
     CDS             complement(3253..>3673)
                     /locus_tag="AC16CM_04775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3139174.1"
                     /translation="TLTILGHKLKGVSVRREYEENLPRVRGNGGELNQVWTNLIDNAA
                     DALDGRGNITLRAYAREGQVMVEVADDGPGIPREAQGQVFEPFYTTREVGARTGLGLN
                     VVRRTVVAHGGSISVRSEPGETRFTVGLPVVGRQNGG"
BASE COUNT          654 a         1238 c         1147 g          634 t
ORIGIN      
        1 tcatgcgggc gagctggccc aacagggcga cgatgcctag aagcccgatc aggaactcca
       61 gcctgaactc tagaccgtgc acttctcccc ccacgacccc aagcggaaga ttacagcccc
      121 ttctctaccc tcgtggctcc atgacgacct cgtccaggta gcaccagatc cagtcctcgc
      181 ccggctgaaa agagcggacg agcgggtggc ccgtggcgtg gaagtgcccc gtggcatgcc
      241 tgttcctgga gctgtcgcaa cagccgacgt ggccgcagat gaggcactcc ctgaggtgta
      301 cccatctgtc tcccatctgc aggcactcct cgcacccttc ggcgctgggc tccacgtccc
      361 gtatctgatc cagatgcgtg catgctccgt ttgccaactc gaaacctcct cgtgagattg
      421 ccttcatgcc gccgccgtct cttcctgcct tccgtccgcc tcctgcaccg gtgccggtcg
      481 gttcttgccc tctataatga tcgccgccgc gaccgcgctc gcgagggccg agcccgcgga
      541 gatgtacatg gccaacctga acgcgcccag aaaagactgt tttacggccg tctcgatctc
      601 ttctgccgta tccccgctaa ccccgggcgg aacctcggcc ccgccaaggt ccactttctc
      661 ggcctcgagt tgctgccgcg cttccgggga caggtcgagt tgctgaacgc gggcgtcgag
      721 gctgcccgag aacacgaaga acacgaagat gccgaggata ggtatggcca gcagacccgc
      781 cgtcctcgag acggcgttgt tgacaccgga ggcgaggccg gagtgcctcc ctgacaccga
      841 gctcaaagcc gtggtcgtca gcggcgctat gacgagcgac ataccgaggc cgagcaccac
      901 gatggcgggg aaaaacgtgg tccagtagga gccctgggta cccggcaagg cgaggagcat
      961 gaaaccaccc gcagcgatga tggggccgat ggtaagcggc agccgcgccc cgaagcggat
     1021 gatcaagccc ccggcccacc tggacatgag gaacgtgatt atggtgaagg gcaggaagga
     1081 gctccctgcg gccgtcgccg agtaatcgtg gacctgtatg aggaggaagg gcagaaagta
     1141 gagcgaccct ccgagccccg cgtagagcag gagcgtcagc acgttggccc ccgagaagtt
     1201 tctggaccgg aacatgccga ggggtagcat gggctcccga gtcctccgct cccacccgac
     1261 aaaaccgatg atgcagatca ccccgagcac gagcgcggcg atcaccctga cgttgtcgaa
     1321 accagccgca gcagaggtta tgagcccgta tacaacccct cccagcccga gcgtggcgag
     1381 cgcggcgcct gggatgtcga gctttctggc gtctgggtcg cggctctcgg ggacgtgctt
     1441 gtaggcgatg acgagcacca ggatcgcgag cggcacgttt atcaggaagg cagcccgcca
     1501 ggagacgttc tcgacgagga agcctccgag gatgggaccc agggccgtcg tcactccaga
     1561 gaggccggcc caggtgccga tagccttgcc gcgctcctcg ccctcgaagg aggagctgat
     1621 gatggccagg gagccgggca ccagcagagc accgcccacg ccctgaacgg cgcgggccac
     1681 gataagcatg cccgggctcg ttgcgagccc gcaccacacc gaggcaaggg tgaagatggc
     1741 cacgccaagc atgaagatgc gcctgcgccc gtagtggtct cctagggagc caccgaccag
     1801 gatgagggcc gacaggaaca gggcgtagga ctccactatc cactgcgcgt cgagggcggt
     1861 ggcgccgagg cgcgtctgta tctgcggcag cgcgacgttc gtaaccgtgc tgtcgataaa
     1921 ggccatggcc gacccgatga tggtcgccgc gaggatccac ggaccagacc gtggtgcgca
     1981 cggggcgtcg gcacgcctgg agcttatgac gcccgcgtcg cacgggggat tacctatgtt
     2041 cgacaaaacg ccggtcctct ccgcggcatc ggtgtccggg ttgtgatccg ctcagaaaga
     2101 ggagccttcc gtggtcagga actcggcctc ctcgggggtc gtctggcggt tcaaaatctc
     2161 gttcctgtgc gggaaccgcc cgaagcgctc gactatgtcc ctgtgggcct cggcgtagga
     2221 gacgacatcg ggcgcgccgg gctcgccggc gagccgttcg aacatctcca ccgagcggcg
     2281 ctgctcctcc acgctctcgc tgtgcatgaa gggcatgtac aggaacattc tctggaaagc
     2341 gggaagctcc ctgtcaagcg cgtgctccac agcgtacttc gaggtcgaga gcgccctgtc
     2401 gtcctccgcg tgggtacgcc catcgccacg gaacatgttg cgtggaaact ggtcgagcac
     2461 gatgaccagc gccaggcaac tctcggcttc ctcgcgccag ccgtcgagct ttccagccgc
     2521 ggcctcctcg tagagttcgg cgaaccttgc ggtgatcgtg gcgtcgaagt cggggtcctt
     2581 gcggaaccac tcctcgcgga actggccgta ttcagggtcg ttctcgctgc cgaaccagaa
     2641 ctccagaacc tctctggccc tactccgcac ggccgatgac cgttatgtct tcttcgctgc
     2701 gggtgaaggt ccgaccctcc acgcggcttc ggtaaccctc gatcatccag agctcgggta
     2761 cgatctccga gaccagctct ccatcgtcga acatcctgac catggagctc tccgagacga
     2821 ccacggagac cgcgctcgtc tgctgggaga tggaggcgcc ggccatgtgg cggctgccca
     2881 gccctagggg caggtccagg ttgtcggagg aggcgtcgat gtagcgggcc gccgacagta
     2941 ccacgcccgc gttcgagacc aggaaagcgc cgtcgagctg ggccagttcc ttgatggtct
     3001 cccgcatgtc atggtgctct atctgcttgt cctcgtcggc gtgaccctgc agggggtcca
     3061 ggatcagggg cttcgagcgc cgcatcacct cacccgagtc cccgaccacg aagagcgtgc
     3121 cgatcttgcg cccctcgcgg ccttcgcgcg cgatctcgac cgccagcgag acggtctgtt
     3181 tgagcaccct tgcgttgacc cgccgcttct ccgagcaaat ctcctcgaat atatgagtct
     3241 ggcccatctc tatcaacctc cgttctgtct gccaactacg ggcaacccga ccgtaaacct
     3301 ggtctcgccc ggctccgacc ggaccgagat gctaccaccg tgggcgacga cggtgcgccg
     3361 caccacgttg aggccgagcc cggtcctcgc tccgacctcc ctggtggtgt agaagggctc
     3421 gaagacctgt ccttgcgctt cgcgcggtat ccccggccca tcgtcggcga cctcgaccat
     3481 aacctgccct tcccgagcgt aggccctcaa cgtgatgtta ccgcggccgt ccagcgcgtc
     3541 cgcggcgttg tcgatcaggt tggtccacac ctggttcaac tcgccgccgt ttccccgcac
     3601 cctggggagg ttctcctcgt actcccgtcg tacggagacg cccttcagct tgtgaccgag
     3661 gatggtcagg gtg
//
LOCUS       DBNGVD010000170         2471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3313967, whole genome shotgun sequence.
ACCESSION   DBNGVD010000170 DBNGVD010000000
VERSION     DBNGVD010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3313967"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="AC16CM_04780"
     CDS             complement(<1..1246)
                     /locus_tag="AC16CM_04780"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3139175.1"
                     /translation="MKTAWTGYGIKTDRSREISARLQRSIPGANTRSLAYFPPYSLAI
                     TSGSGCCIRDADGNEFVDLLNNYTASVHGHAVPAVNAAMAEQAARGTVFPAPAELQAE
                     LAERIIDRVASVEKLRFANSGTEAVMMAVRAARAFTGREKIVKAEGGYDGMWEQVPMS
                     LPGEADPYRTAMPGGVRGLVRMVDFNDVGGLETAMEEEGGEVAAILLEPVTGTGVFEG
                     TAEYFAAARRLADKHGALLIVDEVITLRLAFGGYQEVLGVRPDLTTMGKIIGGGLPVG
                     AFGGRADVMAIFDPSAPHHLHHSGTFNGNLMTMAAGCVTLDLLTAGEIERINALGERL
                     ADGLRRLLAERPDLRGLVNGCGSLVHVDFGTEGEVRNYSDLNLKSPVASRFHLAALEE
                     GLYFAPRGFMNTSTAMDEHVVNE"
     gene            complement(1429..>2471)
                     /locus_tag="AC16CM_04785"
     CDS             complement(1429..>2471)
                     /locus_tag="AC16CM_04785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3139176.1"
                     /translation="LAGFPETVAGVTVNRLCGSGLEAVAQAARSVMLGEAEVYVGGGV
                     ESMSRPPWAVPKPERAFPTGKATMYDTTLGWRMVNPKMEQMGHTDSLGMTAENLAQER
                     FLLTPDEDRAEALASRYDVSREAQDRFALRSHEKALAATDEKRLREEIVPVTVETRGS
                     ETLFEADEGPRRDTSPEKLARLGPAFRKDGSVTAGNSSPLNDGSAAVLVVSRDYAEAN
                     GLRPMARIRSMAVAGMPPRVMGIGPVPATEKALARAGITLADVGLIELNEAFAAQSLA
                     CLHEWGIDAEDERLNPNGGAIALGHPLGASGARILTTLVHEMGGREDVRFGLATMCIG
                     VGQGIAMVVERV"
BASE COUNT          372 a          869 c          792 g          438 t
ORIGIN      
        1 cttcgtttac tacatgctcg tccatcgccg tggaggtgtt catgaaacca cgtggtgcga
       61 agtagagacc ctcctctagc gcggccaggt ggaaccggct tgccacgggg ctcttgaggt
      121 tcaggtcaga gtagttgcgg acttcgccct ccgtcccgaa gtcgacgtga acgagagaac
      181 cgcaaccgtt gacgaggccc cggagatcgg gcctctctgc cagcaaacgg cgtagcccgt
      241 cggcgagccg ttccccgagg gcgttgatgc gttctatctc ccctgccgta agcaggtcca
      301 gcgtcacgca gccggcggcc atggtcatga ggttgccgtt gaaggttccc gagtggtgta
      361 ggtggtgcgg ggcactggga tcgaagatcg ccatcacgtc ggcccgcccg ccgaaggctc
      421 cgacgggcag gcccccgccg atgatcttgc ccatcgtggt caggtcgggc ctgacgccga
      481 gtacctcctg gtacccccca aaggcgagcc tcaaggttat gacctcgtcc acgatgagga
      541 gcgccccgtg cttgtcggcc agtcgccgcg ccgcggcgaa gtattctgcc gtgccctcga
      601 agaccccggt tcccgtcacg ggctccagga gtatcgccgc cacctcgccg ccttcttcct
      661 ccatcgccgt ttcgagcccg ccgacgtcgt tgaagtcgac catgcgcacc agcccgcgga
      721 cccccccggg catggcggtc cggtacgggt cggcttcacc cggcagggac atgggcacct
      781 gctcccacat accatcgtag ccaccctccg ccttcacgat cttctctcgc ccggtaaagg
      841 cccgggcggc gcgcacggcc atcatcacgg cctcggtgcc cgagttggcg aagcgcaatt
      901 tctccacgga ggcgacgcgg tcgatgatcc tttcggccag ctcggcctgc aactctgcgg
      961 gggcgggaaa gacggtaccc cgggccgcct gttccgccat ggccgcgttc acggcgggca
     1021 cggcgtggcc gtggaccgag gcggtgtagt tgttgaggag gtctacgaac tcgtttccgt
     1081 ccgcgtcgcg gatgcaacag ccggagccgg aggtgatcgc cagcgagtag ggaggaaagt
     1141 aggccaggga gcgggtgttg gcaccgggga tcgatcgctg gagccgagcc gagatctcac
     1201 gcgacctgtc ggtcttgatg ccgtatccgg tccaggccgt cttcacgcca ccctcccgtt
     1261 cggaaccaga acacgcccgg agcgtcgctg gtcggtagag atccttctcc tgattctacg
     1321 gcatcgccgg cccttccggc aggcgatagt gatgtgtcct gcaccgtccg gcccgctatc
     1381 atcgaggtct acccctgccc gccctctgcc tcgcggtcca gcaacgtcct atacccgctc
     1441 gacgaccatg gcgatgccct gtcccacccc gatgcacatg gtggcgagtc cgaaccgaac
     1501 gtcttcccta ccccccatct catgcacgag cgtggtgagg atgcgggcgc ccgaggcccc
     1561 cagggggtgt ccgagcgcta tggcgcctcc gtttgggttg agcctctcgt cctcggcgtc
     1621 gatcccccac tcgtgcaaac aggccaggct ctgcgcggcg aacgcctcgt tgagctcgat
     1681 caggccgacg tccgcgagcg ttatgccggc gcgggccaga gccttctctg ttgccggcac
     1741 cgggccaatg cccattaccc gcggcggcat cccggcgacg gccatgctcc tgatcctggc
     1801 catcggccgc aggccgttgg cctcggcgta atccctggag acgacaagca cggccgccga
     1861 gccgtcgttc agagggctcg agttgccggc cgtcacggac ccatctttcc ggaaagccgg
     1921 tccgagcctc gcgagcttct cgggcgaggt gtcgcgcctc ggtccctcgt cggcctcgaa
     1981 gagggtctcg gatcccctgg tctctaccgt cacaggcacg atctcctcgc gcaggcgctt
     2041 ctcgtccgtc gcggcgaggg ctttctcgtg gcttctgagg gcgaagcggt cctgggcctc
     2101 cctggagacg tcgtaacgtg aggcaagagc ctcggcccgg tcctcgtcgg gggtgagtag
     2161 aaaccgctcc tgggcgaggt tctccgcggt catgcccagg gagtcggtgt ggcccatctg
     2221 ttccatcttc gggttgacca tgcgccatcc gagggtggtg tcgtacatcg tggccttgcc
     2281 ggtcgggaag gcccgctccg gcttgggtac agcccacgga ggccggctca tggactcgac
     2341 gccaccccca acgtaaacct ccgcctcgcc gagcatgacc gagcgggccg cctgggccac
     2401 ggcctccagg ccagacccac acagccggtt caccgtgacc cccgccaccg tctcggggaa
     2461 gccagccagg a
//
LOCUS       DBNGVD010000171         6702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_34714, whole genome shotgun sequence.
ACCESSION   DBNGVD010000171 DBNGVD010000000
VERSION     DBNGVD010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_34714"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..784
                     /locus_tag="AC16CM_04790"
     CDS             20..784
                     /locus_tag="AC16CM_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3139177.1"
                     /translation="MSLRPYLEFAVESAYEAGRLTLGYFGAEAARPEFKADDTPVTVA
                     DREAERLIRARIEDRYPGHAVLGEEFGQTGGAGSEHLWILDPIDGTKSFVRGVPLYGV
                     LIGLQIAGTCEVGAAYFPALDEMVCAATGEGCYLNGRRTQVSTRSLDQGTVCFTEVAS
                     FREHGRDAAWERVLDVAGSARGWSDAYGHALVATGRAELMLDPIMNPWDCGPFPPILR
                     EAGGSFGDWSGNETIHANEAMSATTTTLPEALRLIG"
     gene            complement(934..2259)
                     /gene="glnA"
                     /locus_tag="AC16CM_04795"
     CDS             complement(934..2259)
                     /gene="glnA"
                     /locus_tag="AC16CM_04795"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3139178.1"
                     /translation="MADKQSVLQQAQENNVQFIRLWFTDVLGTLKSFAITVEELEDAL
                     DGGMGFDGSSITGYQDIEESDMIAMPDPSTFQVIPWSPQDAPTARMICDVRTPDGDPY
                     VGDPRHVLRRALERANEMGFDKFYCGPELEFFYFRDSKGTEPLDYGGYFDLTTLDAAT
                     ALRRETVQSLQQLGIKVEYSHHEVGISQHEIDLRFDDAMTMADKVMTYKTVVKEVATR
                     HGVYATFMPKPLKDQNGSGMHTHQSLFSGGKNAFFDANDEYYLSSEARSFIAGQLRHA
                     REISIIFAQHVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGQERATRMELRCP
                     DPSANIYLCFAALLHAGLEGIEKGYELPEPMERNLYNLSSEERQKMGIESLPADLGEA
                     IKEAEQSELLHKALGEHTYSRLLQLKREEFEDYRVQVTPYELDKFLPVL"
     gene            2552..3334
                     /locus_tag="AC16CM_04800"
     CDS             2552..3334
                     /locus_tag="AC16CM_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139179.1"
                     /translation="MSERPEEGREERRRTGPLGDDETRQVPADEGSGATRPVTTGRSS
                     GRQAREEKETRVIRSAQRPDTVRDATPYPRGYFEAVEEREDRLRDMYGGVDWLASFLG
                     FVFSIVVASVFSTVVGLVLVPLGFPPEISGGQLGASVLTGLAVLGVLIFLTYFFGGYV
                     SGRLARFDGGRNGAMVLAWTFIVVLIVALVAVVFSGFLPDGAAEIVVGLAQATRSAVD
                     GLAGTGVTGIVVAVAALLLALLGGFFGGRLGSRYHTEIDRAS"
     gene            3340..4374
                     /locus_tag="AC16CM_04805"
     CDS             3340..4374
                     /locus_tag="AC16CM_04805"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HIR3139180.1"
                     /translation="MGRHVLFETGIRRLGSRESGFFYRYPDTGETVREERVLSRIEGL
                     KVPPAWREARIARGPQAKVQAVGYDSAGRVQYLYSARYRERKEREKFERVLRFADALP
                     EMRRVTSNHLRRKGLGPEKVLATMIRLMNVAYFRVGDERYAKDNRTYGIATLRRKHLR
                     IDGDTMTFEYIGKWGMTQRKVVTDRGLRGVVEECVSLPGYEVFKYLDEAGEVRDVKAR
                     DVNLYVKQVMGEEFTPKDFRTWAGTLIAAVKLAELGPVGEPKHANKNVLAAVDAVAER
                     LGNTRDIARASYISPRVIDHYLEGSVVAYHAEYLEEVIAAEQGDLTDGERALLDLLKK
                     ELHRDLRKAA"
     gene            4402..4533
                     /locus_tag="AC16CM_04810"
     CDS             4402..4533
                     /locus_tag="AC16CM_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139181.1"
                     /translation="MKKAIAIKLLKSKTARRVAIKALQNKKVRKIAIDVARKRVMGR"
     gene            complement(4749..5876)
                     /locus_tag="AC16CM_04815"
     CDS             complement(4749..5876)
                     /locus_tag="AC16CM_04815"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR3139182.1"
                     /translation="MNSRDYISLIASDAFETGVREGTLGAEEELWLAAPETLKLASGA
                     QEILDVEPAKLFSGELIDCEIESNTGVHDEPSGVAEDLVMRRRVLLGHARRLGRVLGT
                     SGTHPIGDWREQEIINKPHYQRLKKKLGWLIRRNNTFSLHVHYAIDGRGKAVYLFNRL
                     REYVPHMLALSVNSPFWQGEYTDMHSARVLMFSRAYHRAGLPEPLDSWEEYVTYVDTL
                     QRTGLIDALGEIWWDVRLHPGLSTVEIRAFDAQTSTTRTEALVTLAAALCDMLVSEYE
                     SGDRRPIYTNRDIEENKWSAQRYGLDGAFLNHETREAVETTKAIGTLVARLSASSNRN
                     LSSLERLLEEPTESTRQLEVWHDTGSTLEVARDIAERTSMF"
     gene            complement(5895..6263)
                     /locus_tag="AC16CM_04820"
     CDS             complement(5895..6263)
                     /locus_tag="AC16CM_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139183.1"
                     /translation="MIAPTDYHELLDYRRSVSRLYAGARRETAGSPRNPTARSRRFRV
                     EREALLKGHPQSPLRERDRQNFSGPEYIDYDPALKFSPVVDREVEPGTLEISLQDDGP
                     LRLHRFGKVRYRGGREKLRV"
     gene            6377..>6702
                     /locus_tag="AC16CM_04825"
     CDS             6377..>6702
                     /locus_tag="AC16CM_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139184.1"
                     /translation="MLRAFEGLPEHGRPKQVDLDEMEPSSTPSSPESAVAASTVTAMT
                     LVYSAPTPGTGSRYMDGRVAHESRVLSVVPVRDAIAGSDRRGVGETVLEATRLSLSLT
                     GFADPRG"
BASE COUNT         1207 a         2039 c         2230 g         1226 t
ORIGIN      
        1 ggggtgctat cttacggccg tgagcctgcg accctacctg gaattcgccg tcgagagcgc
       61 ctacgaggcg ggacggctca cgctcggcta cttcggcgcc gaggccgccc gcccggagtt
      121 caaggccgac gacacaccag tgaccgtggc cgacagggag gcggagcgcc tcatccgggc
      181 gaggatagag gaccgttacc ccgggcacgc cgttctaggg gaggagttcg ggcagaccgg
      241 tggggcgggc tcggagcacc tctggatcct tgaccccata gacggcacta aatccttcgt
      301 ccgcggagtg cccctgtacg gcgtgctgat aggcctgcag attgccggca cgtgcgaggt
      361 tggcgccgcc tacttcccgg ccctggacga gatggtctgc gccgccacag gagaaggctg
      421 ttatctcaac ggacgaagaa cccaagtgtc gaccaggagc ctcgatcagg gcaccgtgtg
      481 tttcaccgag gtcgccagct tccgtgaaca cggccgcgat gccgcgtggg aacgcgtact
      541 cgacgtcgcc ggcagcgccc gcggctggtc cgacgcctac gggcacgccc tcgttgccac
      601 cggaagggcc gagctcatgc tcgatccaat catgaacccc tgggactgcg gtcccttccc
      661 gcccatcctc cgcgaggccg gtggctcttt cggcgactgg tctggcaacg aaacgatcca
      721 cgccaacgag gcaatgagcg ccacgacaac caccctgccc gaggcgttgc gcttgatcgg
      781 gtaggacggt gccaggtcgg gcggctcgcg aaccgcccct acatcgttgt gggagttcga
      841 cgttttcgtc gcccgcgtaa cgtcgcgacc acgaacgagg ggtgcgacct tcgccgcacc
      901 cctagctgat agctgaccgc tataggctga cagctacaac accggcaaga acttgtccag
      961 ctcgtacggc gtcacctgca ccctgtaatc ctcgaactcc tcgcgcttga gttgcaggag
     1021 gcgcgagtag gtgtgctcgc caagggcctt gtgcaggagc tccgactgtt cggcctcctt
     1081 gatcgcctcg ccgaggtcgg cagggaggct ctcgatcccc atcttctgcc gctcttcaga
     1141 gctgaggttg taaaggttgc gctccatagg ttcggggagc tcgtagccct tctcgatgcc
     1201 ctccaggccg gcgtggagga gcgccgcgaa gcacaggtag atgttcgccg acgggtctgg
     1261 gcaacggagc tccatgcggg tcgccctctc ctggccggga tggtagagag gaacacggac
     1321 cagtgcggag cggttgcggc ggctccaggc aatgtagacc ggggcctcgt agcctgggac
     1381 caggcgcttg tatgagttca cgtgctgggc gaagatgatg ctgatctcgc gcgcgtggcg
     1441 tagctggccg gcgatgaacg accgggcctc gctggagaga tagtattcgt cgttcgcgtc
     1501 gaagaacgcg ttcttgccgc ccgagaacag gctctggtgg gtgtgcattc ccgagccgtt
     1561 ctggtccttt agcggcttgg gcatgaacgt cgcgtagacg ccgtgccggg tggcgacctc
     1621 cttgaccacg gtcttgtagg tcatcacctt gtctgccatc gtcatggcgt cgtcgaagcg
     1681 gaggtcgatc tcgtgctggg agatgccgac ctcgtggtgc gaatactcca ccttgatgcc
     1741 gagctgctga agggactgca cggtctcgcg ccgcagggcc gtggccgcgt ccagcgtcgt
     1801 caggtcaaaa tagccaccgt agtcgagggg ctccgtgccc ttcgaatccc tgaagtagaa
     1861 gaactccagc tcgggtccgc agtagaactt gtcgaagccc atctcgttcg cccgctccag
     1921 cgcacgcctc agcacgtggc gcgggtcgcc cacgtagggg tcgccgtccg gggtcctcac
     1981 gtcgcagatc atgcgcgccg tcggggcgtc ctgcgggctc caagggatca cctggaacgt
     2041 cgaggggtcc ggcatggcga tcatgtcgga ctcctcgata tcctggtagc ccgtgatcga
     2101 agacccgtcg aagcccatgc ccccgtccag cgcgtcctct agctcctcga ccgtgatcgc
     2161 gaagctcttg agcgtgccaa gcacgtcggt gaaccacaac ctgatgaact gaacgttgtt
     2221 ctcctgcgcc tgctgcaaca cgctttgctt gtcagccaaa gtcgagccta cctcctcgaa
     2281 tttccgttct ttactccagc ccccgggcgg ggacttatcg actacacccg ctatgatatg
     2341 aacctacata cgcgttgcat atggcctggc gtacaagcca ggccgcagga tctggccaga
     2401 tcctcatcct aatgtataaa cgtggcgtct gatatggttg tagacgaccg tatcgggtag
     2461 tcatcggggc ctgaccgatg cgcgcgaagg gtagaatttc aggcactgta ctacagaaga
     2521 tgaatcgccg taatgacata aaggggtcga gatgagcgag agaccagaag agggccgcga
     2581 ggaacgccgc aggactggtc ccctggggga cgacgagaca cgccaggttc ccgctgacga
     2641 gggctcgggg gccacgcgcc ccgtcaccac gggcagatcg tcgggcaggc aggcccggga
     2701 ggagaaggag acgcgcgtga tccggtccgc gcagcgcccc gacaccgtga gggacgcaac
     2761 cccctacccg agaggctact tcgaggccgt cgaggagcgc gaggacaggc tcagggacat
     2821 gtatgggggc gtggactggc tggcaagctt cctcggtttc gtgttctcca tagttgttgc
     2881 ttcggtattc tccaccgtgg tcgggctggt gctcgtgccg ctcggctttc cgcccgagat
     2941 ctccggcggg cagctcgggg cttcggtgct tactggcctc gccgtgctcg gggtcctgat
     3001 cttcctgacc tactttttcg gcgggtacgt gtccggccgc ctcgcccgct tcgacggggg
     3061 acggaacggc gcgatggtgc ttgcctggac gtttatcgtg gtcctcatcg tcgccctggt
     3121 agccgtggtc ttctctggct tcctgcccga cggcgcggcc gagatcgtgg ttggcctcgc
     3181 acaggcgacg agatctgcgg tcgacggcct ggcgggaacc ggcgttaccg gcatcgtggt
     3241 tgccgttgcc gccctgctcc tcgcgctgct tggagggttt ttcggaggac gcctgggaag
     3301 caggtaccac accgagatag accgggcgag ctagccagct tgggccggca cgtacttttc
     3361 gagacgggca tcagacgtct cggttccagg gagagcgggt tcttctaccg ctaccccgac
     3421 acgggcgaga ccgtacgaga agagcgggta ctgtcgcgca tcgagggcct caaggttccg
     3481 cccgcctggc gggaggcccg tatcgcccgt ggcccgcagg ccaaggtgca ggccgtgggc
     3541 tacgactcgg ccgggagggt gcagtacctg tacagcgcca ggtatcgtga gcggaaggag
     3601 cgcgagaagt tcgagcgggt gctccgcttc gccgacgccc tgcccgagat gaggagggtc
     3661 acgagcaatc acctgaggcg caaggggttg ggtcccgaga aggtgcttgc cacgatgatc
     3721 cggctgatga acgtggccta cttccgggtg ggcgacgagc ggtacgcgaa ggacaacaga
     3781 acctacggta tcgccaccct ccgccgcaag cacctgcgca ttgatggcga caccatgacc
     3841 ttcgagtaca tcggcaagtg gggcatgacg cagcgcaagg tcgtaacgga ccgcgggctc
     3901 cgcggcgtgg tcgaggagtg cgtgagcctg cctggttacg aggtcttcaa gtacctcgac
     3961 gaggcgggcg aggtgcgcga cgtaaaggcc agggacgtaa acctgtacgt caagcaggtc
     4021 atgggcgaag agttcacgcc caaagacttc agaacctggg ctgggaccct gatcgccgcg
     4081 gtcaagctcg ccgagctggg acccgtcggt gagccgaagc acgcgaacaa gaacgtgctc
     4141 gctgcggtgg acgccgtagc cgagcggttg ggcaacacgc gcgatatagc ccgcgcctca
     4201 tatatcagcc cgcgggtaat agaccattac ctggagggtt ccgtggtagc ctaccatgca
     4261 gagtacctgg aagaggtcat agcggccgag cagggcgacc ttaccgacgg cgagagggcg
     4321 ctgttggacc tgctgaagaa ggagctccat cgcgatctca ggaaagccgc gtaaaggcag
     4381 agcccgaagg agacgcaaac cgtgaagaag gccatagcca tcaagctgct gaagagcaag
     4441 acggcccgca gggtcgccat aaaagccctg cagaacaaga aggtccgaaa aatcgccata
     4501 gacgtggcaa gaaagcgagt tatgggaaga tagcaagctc cggtgcaccg ttcgccccga
     4561 cccggggtga acctgaaatg agactacgag tttcaggttt cagaaacatc acgaggtgcc
     4621 tgacctccgt ggctcgtacg catctctggc ccatctgtca tcaagaaccg tgctgaatca
     4681 gcaagcgggt gaatcggcca ccttcgatgc cggtgatcaa ctatctgtta ttggctgaag
     4741 gctgatagct aaaacatcga ggttctctcg gctatgtctc gcgcgacctc cagggtggag
     4801 cccgtatcgt gccagacttc gagctggcgg gtggactccg tgggctcttc cagcagcctc
     4861 tccaggctgg agaggttcct gttgctcgac gcggacaggc gtgctaccag ggttccgatt
     4921 gccttcgtgg tctccacggc ctctctggtt tcgtggttca gaaacgcgcc atccaggccg
     4981 tagcgctggg cgctccactt gttctcctcg atgtcccgat tcgtatagat cggcctgcgg
     5041 tcgcccgact cgtactcgga gacaagcatg tcgcaaagcg cggcggcgag ggttacgagc
     5101 gcctcggtgc gggtcgtgga ggtctgtgcg tcgaaggccc gtatctcgac cgtgctcagg
     5161 ccgggatgga gcctgacgtc ccaccagatc tcaccgaggg catctataag gccggtgcgc
     5221 tggagggtgt cgacataggt tacgtattct tcccaggagt cgaggggttc ggggaggccg
     5281 gcgcggtggt aggcgcggga gaacatcagg acgcgggcag agtgcatgtc cgtgtactcg
     5341 ccctgccaga agggggagtt gaccgagagg gcgagcatgt gtggcacgta ctcgcgcagg
     5401 cggttgaaca ggtagacggc cttgccgcgc ccgtcgatag cgtaatgaac gtgcaacgag
     5461 aacgtgttat tgcgccggat cagccacccc agcttcttct tgaggcgctg gtagtggggc
     5521 ttgtttatga tctcctgctc ccgccagtcg ccgatgggat gagttccgct cgtgccgaga
     5581 acgcggccca gtcgccgggc gtgccccaac aggacacgac ggcgcatgac gaggtcttcg
     5641 gccacccccg aaggctcgtc gtggacgccc gtgttcgact cgatctcgca gtctatgagc
     5701 tcgccggaga acagcttcgc gggttcgaca tcgagtatct cctgcgcccc gctggcgagc
     5761 ttgagggtct cgggggcagc cagccagagc tcttcctccg cgccaagggt accctcccgc
     5821 acgcccgtct cgaaggcgtc gctggctatg agggagatgt agtcgcggga gttcaagttc
     5881 ctcctcgggc gattctagac acgtagcttt tctcgcccgc cgcgatatcg gaccttgccg
     5941 aaacgatgta ggcgcagcgg accatcgtct tgcagtgaga tctccagcgt tcccggttcg
     6001 acctcgcggt cgactacggg cgagaacttg agggcgggat cgtaatcgat atactccggg
     6061 cccgagaagt tctgtctatc cctttcccgc aggggagatt gggggtgacc tttcagtaac
     6121 gcttcgcgct cgacacggaa gcgtctgctg cgggccgtcg gattccgggg cgagcccgcc
     6181 gtctctcgcc gcgcccccgc atagagcctg gagacggagc ggcggtagtc gagcaactca
     6241 tggtagtcgg tcggggcgat catctggagg agtatattac cgccccgcaa ggaacgtaag
     6301 ccgacccgcg ttagaatgtt gaaccggtgg caagacagga ggtgctggtg ccaagagaag
     6361 caggagagat aacccggtgc tccgtgcttt cgaggggttg ccggaacacg gacgtcccaa
     6421 acaggtcgat ctagacgaga tggagccttc tagcacacca tcctcacccg agagcgccgt
     6481 ggcggcgagc accgtgacgg cgatgacgct ggtctacagc gcgcccaccc cgggtacggg
     6541 gtcccgctac atggacggcc gcgtggcaca cgagtcgagg gtactatccg tcgtgcccgt
     6601 gcgggacgcc atcgctggtt cggaccggag gggtgtgggt gagacggttc tggaggcgac
     6661 ccgcctgtcg ctctccctga cgggtttcgc cgatccccgc gg
//
LOCUS       DBNGVD010000172         2828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_946536, whole genome shotgun sequence.
ACCESSION   DBNGVD010000172 DBNGVD010000000
VERSION     DBNGVD010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_946536"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..878)
                     /locus_tag="AC16CM_04830"
     CDS             complement(156..878)
                     /locus_tag="AC16CM_04830"
                     /inference="COORDINATES: protein motif:HMM:NF022655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HIR3139185.1"
                     /translation="MRGVLVAVIVPVVLIFLVGPYTFLPSLIEYAVATDVQNRLGIEK
                     RPEVELRSDPPLAMLKGEFSGGRVAMRNVDLGGVRAEKASIDLDPFDIDVWDTMKSGR
                     VVDREPLSGALEIEVSEEEIANLARKNSGMPVTDVSVRKDGVLIDSEASALGTQFPVS
                     VQGDLIVEDGELVFEPEALEAAGVPVPDELADQLLSGADFGYPLDRLPYRTTIDAVET
                     REGKIVLDGRVPSIPLGVYPSG"
     gene            complement(883..1974)
                     /gene="prfB"
                     /locus_tag="AC16CM_04835"
     CDS             complement(883..1974)
                     /gene="prfB"
                     /locus_tag="AC16CM_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3139186.1"
                     /translation="MPELRDRTAELEERLAELEAFFDVEGLRQEAGELGDEMSRPGFW
                     DDPDEARTVSARFSRVQGRIELLDSLRGTLSDSGELLELAEEDVELLSEVEEELGRVE
                     RALEEQEVARLFAGEYDDGDAILTINPGAGGVDSQDWAEMLARMYRRWSERRGFGLEI
                     IEYTEGEEGGIKSATFTVSGEYAFGLLSAERGVHRLVRISPFDAGARRHTSFASVAVA
                     PVVGEAVEVEIEEKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQH
                     QNREVAMRVLRARLFELEREKREQEIAAQSGEKAEIEWGSQIRSYVLHPYKLVKDHRT
                     GEETANVDRVLDGDIDAFIYAYLKNRTAA"
     gene            complement(1974..>2828)
                     /locus_tag="AC16CM_04840"
     CDS             complement(1974..>2828)
                     /locus_tag="AC16CM_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR3139187.1"
                     /translation="RAQEQVESRNFQIRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTMSYVEDALTGVVTEYVSDDVYPEDWDLDALSAEVNRFYPCTIDFKALDVESLTPAE
                     VLGMVLDDARERLEERRTEWQERTTELERLGVARTDGVNTFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKEDYVTYIYRVENIQL
                     REEEMQQLTYSGGEEVPEQQRSASPRKRADAKVGRNEPCPCGSGKKYKKCHGMPGAPP
                     LTREVQGG"
BASE COUNT          438 a         1001 c          836 g          553 t
ORIGIN      
        1 gcgaccgtgt ccctaccgta gaccttggtg acgttgttga atcggatcat gacctctcca
       61 gaattaacct gcgggccgcc aacctcgaag gggtttcgaa gtcccccctc cagggacccg
      121 gccaccgcgg accatagcaa ctaccgccgg cgttatcaac cgctggggta gacgccgagc
      181 ggtatgctgg gcaccctgcc gtcgagcacg atcttgccct ccctggtctc gacggcgtcg
      241 atggtggtcc tgtagggcag gcggtccagc gggtacccga agtcggcccc ggagagcagc
      301 tggtcggcga gctcgtcggg aacagggacc ccggccgcct ccagggcctc cggctcgaag
      361 acgagttcgc cgtcttcaac gatcaggtcc ccctgcacgg agacgggaaa ctgcgtgcct
      421 agggccgagg cttccgagtc gatgaggacc ccgtctttcc gcacgctcac gtctgttacc
      481 ggcatcccgg agttctttcg cgcaaggttg gcgatctcct cttcggagac ctcgatctcc
      541 agcgcccccg acaggggctc cctgtcgacc acgcggccgc ttttcatggt gtcccagaca
      601 tcgatgtcga aggggtcgag gtcgatgctc gccttctcgg cccgcacgcc gccgaggtcg
      661 acgttcctca tggcgacgcg gccgcccgaa aactcgccct tcagcatcgc aaggggcggg
      721 tcgctcctca gctccacctc gggtcgcttc tcgatgccga gccggttctg aacgtcggtg
      781 gcgacggcgt actcgatcag ggaaggcagg aaagtgtagg gccctaccag gaatatcaag
      841 acgacgggca cgatcacggc cacgagcacg ccccgcatgg cgctaggccg cggtccggtt
      901 cttcagatag gcgtagataa aagcatctat gtcaccgtct agcacgcggt ccacgttggc
      961 cgtctcctcg ccggtgcggt ggtccttgac cagcttgtaa ggatgcagca cgtaggaccg
     1021 gatctgggag ccccactcga tctcggcctt ctccccgctc tgggccgcta tctcctgctc
     1081 gcgcttctcg cgctcgagct cgaaaagtcg cgccctcagg acgcgcatgg cgacctcgcg
     1141 gttctggtgc tggctacgct cgttctgaca ctgggccacg atacccgttg ggaggtgcgt
     1201 gatcctgacc gccgagtcgg tcttgttgac gtgctgcccg cctgccccgg aggcccggta
     1261 cgtgtcaacc ttcaggtctt tctcctcgat ctcgacctcg acggcctcac ctaccacggg
     1321 cgcgacggcg actgatgcga agcttgtatg cctcctcgcg ccggcgtcga aggggctgat
     1381 cctgaccagc cggtgcaccc cgcgctcggc agagagaagc ccgaaggcgt actcccccga
     1441 gacggtgaag gtggcgctct tgatgccccc ttcttcaccc tcggtgtact cgataatctc
     1501 cagcccgaag ccccgccgct cggaccagcg tcggtacatg cgggcgagca tctcggccca
     1561 gtcctgcgaa tctaccccgc ctgcgccggg gtttatggtc aggatggcgt cgccgtcgtc
     1621 gtactcgccg gcgaagagcc tggccacctc ctgctcttcg agggcccgct cgacgcgccc
     1681 cagctcctct tcaacctcgg agagcagctc cacatcctcc tcggccagct cgagcagttc
     1741 cccagagtcg gagagggtac ccctcaggct gtcgagcagc tcgatccggc cctgcacccg
     1801 agagaagcgg gccgatacgg tcctggcctc atccggatca tcccagaaac cgggccggct
     1861 catctcgtcg cccagctccc cggcctcctg ccgaagcccc tccacgtcga agaacgcctc
     1921 cagctcggcg aggcgttctt cgagctcggc ggtcctgtcc ctgagctcgg gcactaccct
     1981 ccctgaactt ccctggtcag cggtggcgcc ccgggcatgc cgtggcactt cttgtacttt
     2041 ttgccgctgc cgcacgggca gggttcgttt cgcccgacct tggcgtcggc cctcttccgc
     2101 gggctggccg aacgctgctg ttccggcact tcctcaccac cgctgtaagt tagctgctgc
     2161 atctcttcct cgcgcagctg gatgttctcc acacggtaga tgtaggtgac atagtcctcc
     2221 ttgagccccc gctccatctc ctggaagata tcgaagccct cgcgcttgta ctcgacgagc
     2281 gggtcccgct gggagagccc ccgccagccg atgccctcgc gcaggtagtc catctcgtag
     2341 agatgttccc tccagcggga gtctacgacg gagagaagcg ttctgcgctc ggcttcctcg
     2401 aaggtattga ccccgtcggt cctggcgacc ccgagccgct ccagctcggt ggtccgctcc
     2461 tgccactcgg tcctgcgctc ctccagccgc tcccgcgcgt catccagaac catcccgaga
     2521 acctcggctg gcgtaagcga ctcgacatca agagccttga agtctatggt gcacgggtag
     2581 aagcggttca cctctgccga gagggcatct aggtcccagt cttcggggta gacgtcgtct
     2641 gagacgtact cggtcacgac acccgtcagc gcgtcctcca cgtagctcat ggtgtcgacg
     2701 tcctcgccca tcaagatgtc acgccttatg gcgtagatca cctctcgctg cttgttgagc
     2761 acgtcgtcgt actccaggat gcgcttcctg atctggaagt tcctgctctc gacctgctcc
     2821 tgggcgcg
//
LOCUS       DBNGVD010000173         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1128893, whole genome shotgun sequence.
ACCESSION   DBNGVD010000173 DBNGVD010000000
VERSION     DBNGVD010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1128893"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16CM_04845"
     CDS             <1..200
                     /locus_tag="AC16CM_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HIR3139188.1"
                     /translation="DDSGEGRWTVLEAIHESVPANAIAGSLFARFQSRQEDSYAMKVI
                     AALRGEFGGHAIKEAATEEEK"
     gene            252..611
                     /locus_tag="AC16CM_04850"
     CDS             252..611
                     /locus_tag="AC16CM_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3139189.1"
                     /translation="MKDFHELKVWEKGHELTLAAYKATARFPVEERYGLTSQIRRASA
                     SVPANIAEGCGRGGDTQLARFLQIAAGSASELEYHLLLANQLDMLNSPEHKNLTGQVT
                     EVKRMLTSFIKKLKADS"
     gene            611..2155
                     /gene="zwf"
                     /locus_tag="AC16CM_04855"
     CDS             611..2155
                     /gene="zwf"
                     /locus_tag="AC16CM_04855"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3139190.1"
                     /translation="MADSLQAESLQYALRDEQRLPRVPEPGVMVIFGASGDLTARKLV
                     PALYDLAAQRRLPLEFAVVGISRTEMSHEEFREKLRKMLEEQRSGEVSDDVWESFSNG
                     IFYMAGDSNKQETYDELKELLEKLDSERGTQGNRAFYLSSSPSLFPAIVQHLGDAGMN
                     REEDGAWSRLVVEKPFGRNLESAEELNAELQRYFDETQIYRIDHYLGKETVQNILALR
                     FSNGIFEPIWNQHYVDHVQITVAEDIGVGTRGAFYEEAGALRDIVQNHVMQVLCLTAM
                     EPPVSFDAESVREEKVKVLKAVRPIPGDDVDAYAVRGQYEQGWVFGEEVSGYREEENV
                     DPESVTETFVALKVFVDNWRWAGVPFYIRAGKRMPKKATEVAIQFHSSPHTPFARDDT
                     EGLEPNVLVVRVQPEEGLSLKIGAKVPGSGFEVSSVNMDLLYGTAFLEEAPDAYQRLL
                     LDLMLGDPTLFIRADEAEGAWRILDPVMHHWAEEKSVHFYAAGTWGPPEADELLGQDG
                     REWRRP"
     gene            2140..>2996
                     /locus_tag="AC16CM_04860"
     CDS             2140..>2996
                     /locus_tag="AC16CM_04860"
                     /inference="COORDINATES: protein motif:HMM:NF021632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HIR3139191.1"
                     /translation="MEKAVIGEARKVPGGGRMSVGQIEHELGKLRTNDDGSLALRSSV
                     LNLIVVTDEDSAEEITTSVSRLAGRYPARAVVFIADPEDEPNVDVGLSAFCHVRGGGG
                     AQVCAEQITIHAEGPPAEHLESFAGPLLIPDLPVFLWYPGAFSSRATGFSAMAQLADR
                     VILDSAGGPTEDHGACLREISNLLEDPATPAIGDLQWVGLSPWRSLLADTFGVRERAG
                     ELEKIERAEVLYAPGGENRALLLVGWLASTLGWTPESTTRDGGDRTIEFSGPSGGITV
                     ELSPDSPDA"
BASE COUNT          570 a          931 c          996 g          499 t
ORIGIN      
        1 tcgacgactc gggcgaggga cgctggacgg tcctcgaagc catccacgag tccgtcccgg
       61 ccaacgccat agccggctcc ctcttcgccc gcttccagtc ccgccaggaa gactcctacg
      121 ccatgaaggt aatagccgcc ctccgcggcg agttcggtgg gcacgcgatc aaagaggccg
      181 caacggaaga ggagaagtga ggtcatcagc cttcagctat cagcattttt accaggtgcc
      241 gataatcgtg catgaaggat tttcacgagc ttaaggtctg ggagaaggga cacgagttga
      301 ccctggctgc ctacaaggcc acggcgaggt ttccagtcga ggagcgttac gggctgacga
      361 gccagataag acgcgcgtct gcgtccgtgc ctgccaacat cgcggagggt tgcggcaggg
      421 gcggcgatac gcagctggcg cgcttcttgc agatcgcggc gggatcggcg agcgagctcg
      481 aataccatct gctgttggcg aatcaactag acatgttgaa ctctccggag cacaaaaatc
      541 ttacagggca agtgacggag gtcaagcgaa tgctcacatc gttcatcaag aagctgaagg
      601 ctgatagctg atggctgaca gcttgcaggc cgaaagcttg caatacgccc tccgcgacga
      661 acagcggctt ccccgggtgc cggagcctgg ggttatggtg atcttcggcg cgtctggtga
      721 ccttaccgcc cggaagcttg ttcccgcgct ctatgatctg gcggcgcagc ggaggctgcc
      781 cctggagttc gcggtcgtgg gaatctcgcg gaccgagatg agccacgagg agttccgcga
      841 gaagctacgc aagatgctgg aggagcagcg ctccggggag gtctccgacg acgtctggga
      901 gtccttttcg aacggcatct tctacatggc gggcgactcg aacaagcagg agacctacga
      961 cgagctcaag gaacttctgg agaagctcga cagcgagagg ggaacgcagg ggaacagggc
     1021 cttctaccta tcttcttcgc cctcgctctt ccccgccatc gtgcagcacc tcggggatgc
     1081 cggcatgaac agggaagaag acggcgcctg gtcccggctc gtcgtggaga aacctttcgg
     1141 gcggaacctg gagagcgccg aggagttgaa cgctgagctc cagcggtact tcgacgagac
     1201 ccagatctac cgcatcgacc actacctggg caaggagacc gttcagaata ttctggcgct
     1261 aaggttctcg aacgggatct tcgagccgat ctggaaccag cactacgtgg accacgtcca
     1321 gatcacggtc gcagaggata tcggggtcgg cacgcgcggg gccttctacg aggaggccgg
     1381 cgccctccgc gacatcgtcc agaaccacgt gatgcaggtg ctctgcctta cggcgatgga
     1441 gcccccggtc tccttcgacg cggagtcggt tcgcgaggag aaggtgaagg tcctgaaggc
     1501 cgtccgcccc atccccgggg acgacgtgga cgcatatgcg gtacgcggac agtacgagca
     1561 gggctgggtg ttcggcgagg aagtctccgg ctaccgggaa gaggagaacg tggaccccga
     1621 gtccgtaacc gagaccttcg tggcgctcaa ggtcttcgtg gacaattggc ggtgggccgg
     1681 cgtgcccttc tacatcaggg ctggcaagcg catgcccaag aaggcgacgg aggtcgcgat
     1741 acagttccac tcctcgcccc acaccccgtt cgcccgcgac gataccgagg gcctcgagcc
     1801 gaacgtgctg gtggttcgcg tgcaacccga ggaggggcta tccctcaaga taggggccaa
     1861 ggttccagga tccgggttcg aggtgagctc ggtaaacatg gacctgctct acgggacggc
     1921 cttcctggag gaggcccccg acgcctacca gcggctgctg ctcgacctga tgctcggcga
     1981 ccccacgttg tttatccggg ctgatgaggc cgagggcgcc tggaggatac tcgaccccgt
     2041 catgcaccac tgggcggaag agaaaagcgt gcacttctac gcggcgggaa cctggggacc
     2101 acccgaggcc gacgagcttc tggggcagga cggcagggaa tggagaaggc cgtgatcggg
     2161 gaggcccgca aggtccccgg cggcgggcgg atgagcgtgg ggcagataga gcacgagctt
     2221 ggcaagctcc gcaccaacga cgacggctca ctcgccctcc gctcaagcgt gctcaacctc
     2281 atcgtggtga cggacgagga ttcggccgag gagatcacga cctccgtctc caggcttgcg
     2341 ggccgttacc cggcgcgggc cgtggtgttc atcgccgacc ccgaggatga accaaacgtt
     2401 gacgttggcc tctcggcctt ctgccacgtc aggggaggcg gtggcgcgca ggtgtgcgcc
     2461 gagcagatca ccatccatgc cgagggtcca cccgctgagc acctggagag cttcgccggc
     2521 ccgctcctga tccccgacct ccccgtcttc ctctggtatc ccggagcgtt ctcatcccgc
     2581 gcgactgggt tctccgccat ggcgcaactg gccgaccggg tgatccttga ctcggccggg
     2641 ggaccaactg aagaccacgg ggcctgcctg cgtgagatct ccaacctcct cgaagatccg
     2701 gcgacgcccg ccatcgggga cctgcagtgg gtggggctct caccctggcg ttcgctgctc
     2761 gccgatacct tcggcgtccg cgagcgggcg ggagagctgg agaagatcga acgcgccgag
     2821 gtgctctacg cgcctggcgg agagaacagg gccctcctgc tcgtcggctg gctcgcctcg
     2881 accctgggat ggactccgga gagcacgacc agggacggcg gtgaccggac catagagttc
     2941 tccggtccct ccggcggcat caccgtcgag ctctcccccg attcaccgga cgcgag
//
LOCUS       DBNGVD010000174         4545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4955599, whole genome shotgun sequence.
ACCESSION   DBNGVD010000174 DBNGVD010000000
VERSION     DBNGVD010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4955599"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..2690
                     /locus_tag="AC16CM_04865"
     CDS             336..2690
                     /locus_tag="AC16CM_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR3139192.1"
                     /translation="MAKAGILRLSVIVAAVFMASLLVLALTGLADAASSSEAPGAPGK
                     KAVWTAADKDGFGTSTTTTSKVWYTLNDGKLTEVYYPDLGTPSVRDLQFVVSDGKTFA
                     ELETKATTHKVRLVDDRALEYRQINTDRDGKYRITKTYVTDPARSTVLVDVTFESLTG
                     KPYRLYALYDPSLNNGGDNDTATSKDGQLLASDGKVASALIGSPEFGPSSSGYLGTSD
                     GWTDLKNDYKMDWNFRSASTPGNVVQTAKTALTGLSGDRRLTLALGFGATRSGALSTA
                     RGSLTGGFQEAQAGYRAGWHGYLGSLKEAPASVDTSLQRTTYDVSAMTLAAHEDKTYR
                     GAYVASPSMPWVWGTTSQETGDILDSRGPDETSGAYHLVWSRDLYQIATALMAAGDRA
                     GANRALTYLFERQQKPDGSFPQNSTVDGSEHWENVQLDQVAFPLVMAWQLGRKDGATY
                     RDHIKPAADYIVDHGPATPQERWENQGGWSPATIASEIAGLVCAAKIARLNGDEASAT
                     TYMSKADNWQRNIEEWTVTTNGPLADHPYYLRITKSPGDDPADSDVPPNPNAPTTYSI
                     GDSGPSTIDQRLVVDTSYLELVRLGVKPADDPAIVRTLNVVDSKAIVGPFPTPPNEPQ
                     KRGLRVDTPNGTFWHRFNFDGYGETRDGGPWDIGYPSCDTLPRPCLQSQRTIGRAWPI
                     FAGERGEYELAAGGSGAADSASERLASIAKTGNAGYMLPEQVWDDFPPSGRSGFPKGE
                     GTLSATPLAWTHAQYVRLAWSIQVGHPVEQPRVVSCRYAGRCAR"
     gene            complement(2702..2890)
                     /locus_tag="AC16CM_04870"
     CDS             complement(2702..2890)
                     /locus_tag="AC16CM_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139193.1"
                     /translation="MSQLAWAHAHETLLWASKHKRSRHTFNYDHINGPDPTSQLSSVW
                     HIPAVPMREKTHGRRAVP"
     gene            complement(3070..3933)
                     /locus_tag="AC16CM_04875"
     CDS             complement(3070..3933)
                     /locus_tag="AC16CM_04875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139194.1"
                     /translation="MTPPGEMSGHHETARQQSRATWKAVASGWYAQREGIWKASRPVT
                     QWMVRKLDPQPGDTVLELAAGLADTGLMAARLVGESGRVIVTDFTPEMVAAARRRAEE
                     LGVQNAEFRVLDAERMDLETDSVDGVLCRWAYMLMIDPSAAFAETRRVLRPGGRLAFS
                     VWAARVRNPAISLVGRVLESQGHIPPPDPRAPSAFAMADPGRIREFVLGAGFAEPEIE
                     EVAFRWPFADQDAYWRYLSETAASVSPVLRALSPEAQKAVREQVEEAARPYRSGEGYD
                     FPAVCLNAVTY"
     gene            complement(4311..>4545)
                     /locus_tag="AC16CM_04880"
     CDS             complement(4311..>4545)
                     /locus_tag="AC16CM_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139195.1"
                     /translation="CLPPTDKGGRMEGSSGNVVLSCGGCGERTTLGDPLSVWRSGRTS
                     FECRCGASLTLSDRLQPTDTNEQTEIGTAALCR"
BASE COUNT          845 a         1503 c         1463 g          734 t
ORIGIN      
        1 aacttctccc aaaacggcgc gtcagtacta cgcccgcgcg tacctgcact cggcctcccc
       61 atctcccgcc aggcagctca agcatgttcg gtgccctcgc ctctcacgcc gagtcccgga
      121 cccggcagcc ccgagctgtt gcggatctcc gtactgcccg ggtagcgtaa ggaggcgtgg
      181 acgccacctc tgtgcgcgtt gctctgttta gtcgaacttc atgcgctctg tgtggaggtg
      241 gggttgcgcc atttgtaagc tcaagtgtat gatgccgttg ttacgtgtac aggggcaagg
      301 ggcggtttcc cgttcagtga cataggggga aaggcatggc gaaggccggg atactcaggc
      361 tatcggttat cgtcgcggca gtctttatgg caagcctgct ggtgcttgcg ctcaccggat
      421 tggccgacgc tgcgtcgtct tcggaagctc ccggggcccc gggaaagaag gcggtgtgga
      481 cagccgcaga caaggacggc ttcggaacct ctacgaccac gaccagcaag gtctggtaca
      541 cgctgaacga cgggaagctc accgaggtct actatcctga tctgggcacg ccgagcgtgc
      601 gcgacctgca gttcgtggta agcgacggca agactttcgc cgagctcgag accaaagcta
      661 ccacccacaa ggtacggctc gtggacgaca gggcgctcga gtaccgccag atcaacacgg
      721 accgagacgg caagtacagg atcaccaaga cctacgtgac cgaccccgcc cgctcgacgg
      781 tgctcgtcga cgtgaccttc gagtcgctaa ccggcaaacc ctaccggctc tacgcgctct
      841 acgatccctc gctgaacaat gggggcgaca acgacaccgc caccagcaag gacggacagc
      901 tgttggcgag cgacggcaag gtggccagcg cgctcatagg atcccccgaa ttcggaccga
      961 gctccagcgg ctacctcggt actagcgacg gttggacgga cctcaagaac gactacaaga
     1021 tggactggaa cttccgctcg gcctcgacgc ccggcaacgt ggtccagacc gccaagacag
     1081 cgctcaccgg actctccggc gaccggcgcc tgacgctcgc cctgggcttc ggggctacgc
     1141 gctcgggagc cctctccacg gcgcggggtt cgctcacggg cggcttccag gaggctcagg
     1201 ccggctaccg agccggctgg cacggctacc tcggctcgct caaggaagcc ccggccagcg
     1261 tagacacatc cttgcagcgc acgacctacg atgtctcggc catgaccctc gctgcccacg
     1321 aggacaagac ctacaggggc gcctacgtcg cctcgccttc gatgccgtgg gtctggggaa
     1381 cgacctccca ggaaactggg gacatcctcg acagcagggg gccggacgag acctcggggg
     1441 cctaccacct cgtctggtcg cgcgacctct accagatcgc gactgcgctc atggcggccg
     1501 gcgacagggc cggggcgaac cgcgccctca cctacctctt cgagaggcag caaaaacccg
     1561 acggatcgtt cccccagaac tccacggtcg acggcagcga gcactgggag aacgtacagc
     1621 tcgaccaagt ggccttcccg ttggtgatgg cctggcagct cggacggaag gatggagcga
     1681 cttaccggga tcacatcaag cccgcggcgg actacatagt agaccatggg ccggccacgc
     1741 cccaggagcg gtgggagaac cagggcggct ggtcgcctgc cacgatcgcg tcggagatag
     1801 ccggtctggt gtgcgcggcg aagatagcgc gcctcaacgg cgacgaggct tcggccacaa
     1861 cttacatgag caaggcggat aactggcagc ggaacatcga ggagtggacc gtcaccacca
     1921 acggcccgct cgccgaccac ccctactacc tacgcatcac caagagtccc ggcgacgatc
     1981 ccgccgactc cgacgtcccg cccaacccca acgccccgac cacctactcg ataggcgaca
     2041 gcggtccctc gaccatcgac cagcggttgg tggtcgacac cagctacctg gaactggtca
     2101 ggctcggcgt caaaccggcc gacgaccccg ccatcgtgcg gaccctgaac gtcgtcgact
     2161 ccaaggcgat cgtgggcccc ttcccgaccc cgccgaacga gccgcagaag agggggctca
     2221 gggtggacac cccgaacggc accttctggc accgcttcaa cttcgacggc tacggtgaga
     2281 cgcgcgacgg ggggccgtgg gacatcggct acccctcgtg cgacacgctt ccgagaccct
     2341 gcctacaaag ccagaggacg atcggccggg cttggccgat cttcgctggc gaaaggggcg
     2401 agtacgaact tgccgcgggg ggttcgggcg cggcggactc cgcctcggag cgtttggcgt
     2461 cgatcgccaa aaccggaaac gccggctaca tgctgcccga gcaggtgtgg gacgacttcc
     2521 cgccctcggg tcgatccggc ttccccaagg gggagggcac cctttcggcc accccgctgg
     2581 cctggacaca cgcccagtac gtgcgcctgg cgtggtccat ccaggtagga cacccggtgg
     2641 aacagccccg ggtagtctcc tgccgctatg ccgggcgttg cgccagatag gagcttatcg
     2701 gctacggcac agcacgacgg ccgtgggtct tctctctcat gggcacggcg gggatgtgcc
     2761 agaccgagga gagctgggag gtggggtctg ggccgtttat gtggtcgtag ttgaaggtgt
     2821 ggcgcgagcg tttgtgtttg ctcgcccaca ggagggtttc gtgggcgtgg gcccacgcaa
     2881 gttgggacaa cgccgggctt aaacttctgt gcccggctgg cgtggccagg agatcacgat
     2941 acttgggatc gccgcgctct agacgttggc agcggcatcc cgcgaggagg cggggcgaat
     3001 cgcggtgaga taacgcgaac cgggctacct acggacaaag ctacacggaa ctgtagagtc
     3061 ggcccaggtt tagtacgtta cagcgttaag gcatacggcc gggaagtcgt agccttcgcc
     3121 agatcggtac ggtcgagccg cttcctccac ctgctcccga accgccttct gcgcttcggg
     3181 ggatagggct cgcaagacgg gcgagacgga tgcggccgtc tcgctcagat aacgccagta
     3241 ggcgtcctgg tcggcgaaag gccagcgaaa agcaacctcc tctatctcgg gctcggcaaa
     3301 cccggcaccc aaaacgaact ctcggatgcg ccccgggtcg gccatggcga aggcgctggg
     3361 tgccctaggg tcgggtggcg ggatatgccc ctgtgactcc agtaccctgc ccacgagcga
     3421 gatagccggg ttgcgtacgc gggcggccca gacggagaaa gctagacgac cccccgggcg
     3481 tagtacgcgc cgggtttccg cgaaagccgc agaaggatcg atcatcagca tgtaggccca
     3541 gcggcagagg acgccgtcga cgctatccgt ttcgaggtcc atacgctcgg catcgagcac
     3601 cctaaactcc gcgttctgca cgcccagctc ctccgccctt cgacgagccg cagcgaccat
     3661 ctcgggcgtg aaatccgtga ctatgacacg ccctgattcg cccaccaagc gagccgccat
     3721 gagcccggtg tcggccaggc cggcggccag ttcgagcacg gtgtcccccg gctgcgggtc
     3781 gagttttcgg accatccact gcgtgaccgg acgagaggcc ttccagatgc cttcgcgctg
     3841 agcgtaccag ccggaggcca cggccttcca ggtggccctg ctttgctggc gtgccgtctc
     3901 atggtgcccc gacatctcgc caggtggtgt catgccgatc tcccttgcct tctcgttcgt
     3961 gtaccaagat agagagccga caagatcagc gcaagctgat tgcgttcaga ccgtctccaa
     4021 gacccctata agattcgcga cgttcgctga ggcattccgc tgtggcggaa tcatcggatg
     4081 cgaggcgggc accgttgtcg ccaccgaccg cgtgtcggcc gacgccgatc acgacaacgg
     4141 cgctaccctt tacggtgcag ctcgatacgg aagggacgat gagcgtacga ctcgtcacct
     4201 aagcaccgac ggccaaagag ggggtttctc cgcttggctg cactcggggc gcggatcgag
     4261 gtcggcacgt ggggggcggg gatggcggtg gctacggggg aggtgatccc ctagcggcag
     4321 agcgctgcgg tgcctatctc ggtttgctcg ttggtgtcgg tgggctgaag ccggtccgag
     4381 agcgtgaggc tggccccgca cctacactcg aaggaggtcc tcccggagcg ccacacagag
     4441 agcggatctc cgagcgtggt cctctccccg caccccccgc acgacagcac aacattccca
     4501 gaagatcctt ccatgcgacc ccctttgtcc gttggcggca gacaa
//
LOCUS       DBNGVD010000175         7847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4226671, whole genome shotgun sequence.
ACCESSION   DBNGVD010000175 DBNGVD010000000
VERSION     DBNGVD010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4226671"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC16CM_04885"
     CDS             complement(<1..437)
                     /locus_tag="AC16CM_04885"
                     /inference="COORDINATES: protein motif:HMM:NF047111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3139196.1"
                     /translation="MSWGAFHGARALETERFVPGQIVIKLAPGVNLSELNLSSYGLRV
                     EETFLGQANTNVYLLKTKNDANLDDTLAVLRKQVSLIAYAELNYVVDAPEAAGRHRAF
                     PAGDATPTTGNYSRNGAYSDSALNLTAARKISKGAGTRVAVLDT"
     gene            complement(605..1018)
                     /locus_tag="AC16CM_04890"
     CDS             complement(605..1018)
                     /locus_tag="AC16CM_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139197.1"
                     /translation="MNACEDHVGETTRVLLVEDHALFRQAMSHLLHQHSRMEVSQAGT
                     LAEARHLLSGVDVVALDDYLPDGAGIDLIGEVRASNPKASVLVLNDAPDSANRRLALA
                     AGADSVVSKVESPFTIIDEITCLGCARQVCNHTNS"
     gene            1285..1953
                     /locus_tag="AC16CM_04895"
     CDS             1285..1953
                     /locus_tag="AC16CM_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139198.1"
                     /translation="MSGGLVRLGGVCGLLFVVLMLPAYLVGSPDAATPLSAPREVLGY
                     FGTSAENFVFFNGSLILLCPFFFLWFLGVLYGVLRRAEGVDGVLSTVMLVGGIFFIGL
                     QCVGNAVEILHPAAVQRFVNFVPDPQLAFASLALSTWLYHFCQVGDAVMIFAASLVVL
                     GSGILPRWLALVGLLAAILALLHLLLPLIGSLSGLVWVALVSVLMLTGLSQGTQAPRR
                     RARR"
     gene            2020..2781
                     /locus_tag="AC16CM_04900"
     CDS             2020..2781
                     /locus_tag="AC16CM_04900"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3139199.1"
                     /translation="MRENRLRAIWGDGGTVLNGWVHIPSPFSAEVLAHSGLDSLTVDM
                     QHGPVDYADLVTMLQAISTTDTVPVVRVPWHDPGLVMRALDAGAYGVICPMVDTPGQA
                     ESFVGACRYPPDGYRSYGPFRAAIYGGEDYTEHANQTVVAIAMIETREAIENLDGILA
                     VDGLDGVFVGPSDLGQSLGYGPGTDRDEPEVVEAIDRVVSTARDRGLAAGIFTGTPEH
                     ASRMIEKGFNFVNVSTDVQLLAAASAGVVTQLRGE"
     gene            2829..3086
                     /locus_tag="AC16CM_04905"
     CDS             2829..3086
                     /locus_tag="AC16CM_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3139200.1"
                     /translation="MAQRETTVGPEAGIHGRPAARILKAAKSFSSQISLVKGDREVNA
                     KSIIKITGFARKGEQVIVRAEGDDAEQAVETVAAEISADED"
     gene            3102..4730
                     /gene="ptsP"
                     /locus_tag="AC16CM_04910"
     CDS             3102..4730
                     /gene="ptsP"
                     /locus_tag="AC16CM_04910"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3139201.1"
                     /translation="MKLSGVGASEGVAVGPAFVPRTGGSEPERTGIGEREVEAEIGRF
                     GDAVRVVAARISETAEGLRESGNEDEAGIFDFHAEMAEDPELASAVEERVKNLQSPEA
                     AVLAAGEEYAAEFEAIEDEYLSSRADDVRDVARQISAELMGQADSGFEALYEPSVVLA
                     KSLAPSDTARIPKGLALAFVTAEGSRTSHVSIMARSMGIPAVVGVGDALSGALEAETV
                     AVDGTEGSAITDPDPETVARFDKLSQALAEERVALDQYRHAEARTADGLRIEVAANLG
                     SASEAEEALEWGAEGVGLFRTEFLFMERDDLPTEEEQYDAYSTVARAFGHKPVIIRTL
                     DVGGDKNLPGVDQIHEDNPFLGWRGIRMSLDTPDLFKPQLRALLRAAAHGNVKVMFPM
                     VVEAGELLAAKEMLAEARRELEEQGADVGEIETGVMIETPAAAVCAAELASEAAFFSI
                     GTNDLVQYTLAADRGNERLRRLQGADHPAVLALIRQTCSAAQEANIPVGVCGEAAGDP
                     QLIPNLLDLGVTELSMAAPSIPKAKKVVSEWKRT"
     gene            4777..5517
                     /gene="nagB"
                     /locus_tag="AC16CM_04915"
     CDS             4777..5517
                     /gene="nagB"
                     /locus_tag="AC16CM_04915"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR3139202.1"
                     /translation="MKARIFPDYEDMSAAAADAVIRRVSEKPTSVFLLPTGTTPLGMY
                     RRLVDVYRLGGVSFAGAAFFNLDEYLGLVPDHPASYHVYMKDHFYGLIDADPARIFVP
                     DGAAPDPVAECERYEAAIEAFGGVDMCVLGIGRNGHIGFNEPGASFHSRTRVVTLSES
                     TRLVNSADFEANRAPERAITVGMATIAESREVLLLASGTNKASAVAAALEGEVSEDMP
                     ASMLGEHARATMFLDEDAASGLGQGAAG"
     gene            5660..6655
                     /locus_tag="AC16CM_04920"
     CDS             5660..6655
                     /locus_tag="AC16CM_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3139203.1"
                     /translation="MRVGLLGAGRIGVLHAGLLACDPRVKEFLVGDADERRAESIAAE
                     VGGRSGSIEDILRSGPDCVVIAASTQAHAPLILACVEAGIPAFCEKPIALGYSDTVEV
                     VEAVESSGAVLQIGFQRRFDAGYAEARRLVESGELGTIYSVRLATHDPEPPPEEYIAG
                     SGGIFRDLHIHDFDVLRWLTGAEAREVYAAGGVRGFGFFGEHGDVDTSAALITMQDGS
                     VAVLTGGRHDPLGYDVRAEIFGSRDSVAVGVNGRTPLRSVEPGVQPSGKVAYPDFQNR
                     FLDAYRAELEHFLSLARGEAENRCTARDALEALKIAMAADLSLAEHRPVMVSEVG"
     gene            6727..>7847
                     /locus_tag="AC16CM_04925"
     CDS             6727..>7847
                     /locus_tag="AC16CM_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3139204.1"
                     /translation="MGTSVPRGLSRRDFLKTSGAGLAGAVLLGAAGCGGAEQGGGVTE
                     LYYTASPDESGTTQKLIDKFNQQNKGTYKVIFREGNSDTGQRLDKLRTQFQAGGEELD
                     VILGDVIWTAELAANGWVTDLTDRFPQSTQKEYLPGSVEAIIYEDKPYAMPWYTDTGL
                     LYYRKDLLEQSGFDAPPKSWDELKTMALKVMDQSGTKFGFVFQGARYEGGVCDGCEFI
                     WGHGGNVLDPEDPNKVIVDSPEAIAGLATERSMITDGVSPQAVTVYKEDESAGAFLNG
                     DAVFLRNWPYVYALIGTSDYPKLDTDQVGVSELPSGDGKPGNGTVGDQPLYISSSSKN
                     PDAAWKFIEFLSSPEQQKLRAIEGSYLPTRSDLYDDPEI"
BASE COUNT         1370 a         2467 c         2643 g         1367 t
ORIGIN      
        1 gtgtcgagca ccgcgaccct cgtgccagcg cctttgctga tctttcgggc tgccgtgagg
       61 ttgagtgcgg agtcggaata ggcgccgttc ctggagtagt tgccggtagt gggcgtcgca
      121 tcgccggccg ggaaagccct gtgcctgccc gcggcctcgg gcgcgtcgac gacgtagttt
      181 agttcggcgt aagcgattag cgagacttgc ttcctcaaaa cagcgagcgt gtcgtccagg
      241 ttcgcgtcgt tctttgtctt gagcaggtag acgttggtgt ttgcctggcc gaggaatgtt
      301 tcctccaccc tcaggccgta actactgagg ttcaactcgc tcaggttaac cccgggggcg
      361 agtttgatga ctatctggcc aggaacgaaa cgctccgtct ccagcgccct ggcgccgtgg
      421 aacgcacccc atgacaccaa caaacagagc gctacgagta atgccgccag ggttgcaaaa
      481 gcttgtgatg atgctttggt aatcatagtc ggttcccgag acctcgttgt tcgttcgccg
      541 gacagcacct actcttctcg aggcattata gactatgggt tggcacgaag tgtccccgcc
      601 gtggctagct gtttgtgtga ttgcatacct gccgggcgca gccgaggcag gtgatctcat
      661 cgattatcgt gaagggagac tcgaccttcg agaccacgct atccgccccg gccgcgaggg
      721 ccaggcgcct gttcgccgaa tccggcgcat cgttcaggac gagaaccgag gccttcgggt
      781 tgctggcgcg cacttccccg atcaggtcta tgcccgcccc gtctggcagg tagtcgtcca
      841 gcgccacgac gtcgacgccg ctgagcaggt gccgcgcctc cgccagggtg ccggcctgcg
      901 agacctccat acgagaatgt tggtgcagca ggtgggacat cgcctgccga aaaagggcat
      961 ggtcctcgac cagcagcacc cgggtcgttt ccccgacgtg gtcctcgcaa gcgttcatgc
     1021 ggttcctccc gattctgctg ctagctttcg gcggccggct ctgggccgac caagaaatga
     1081 agtcgcgatg catcgtccct ttttgtgacg gtatgcaacc ctatatatgc ctccggctgc
     1141 ctcccctgat acgccgctcc cgggggttct acccgcggta cgatccgttc cggccggaaa
     1201 ccgagcgggt aggggaggac gaggttaccc gaaatcccct gtagaatgca ttgttgccag
     1261 gggtcggtga ggaggaggaa gcttatgtct ggtgggttgg tgaggttggg tggcgtttgc
     1321 ggcctgttgt tcgtggtgtt gatgttgccg gcatatctgg tcggctctcc cgacgccgcg
     1381 accccgctct ctgcacccag ggaggtgctc gggtatttcg gcacatcggc ggagaacttc
     1441 gtgttcttca acgggtctct catcctactc tgtcctttct tctttctgtg gtttctcggg
     1501 gtgctctacg gggtgctgcg ccgggccgag ggggtagacg gggttctctc cacggtgatg
     1561 ctggtcggcg ggatcttctt tatcggcctc cagtgcgtcg gcaacgcggt agagattttg
     1621 caccccgccg cggtgcagag gttcgtcaac tttgttcccg acccccagct agccttcgcc
     1681 tcgctggccc tctcgacgtg gctataccac ttctgccagg tgggtgacgc ggtaatgatc
     1741 ttcgcggcct cacttgtggt gctcgggagc ggcatcctgc cgcggtggct ggcgctggtt
     1801 ggtcttctgg ctgccatcct ggccctgctc caccttctgt tgccgctgat tggctccctc
     1861 tcagggctcg tgtgggtggc cctcgtctcc gtgctgatgc tgaccggcct gtcgcaaggc
     1921 acccaggcac ccaggcgacg cgcgagacgc taacggcacc ccggccgggg atgctctagg
     1981 ctacgaggct ctggtccagc attcgagagg gggcaattgg tgagggagaa tcgtctgagg
     2041 gccatctggg gcgacggggg tactgtcttg aacggctggg tgcacatccc gagccccttc
     2101 tccgccgagg tgctggccca ctcgggcctg gacagcctga cggtagatat gcagcacggg
     2161 cccgtagact acgcggatct ggtcaccatg cttcaggcca tctctaccac ggataccgtg
     2221 ccggtggtac gggtgccgtg gcacgatccc ggcctcgtta tgcgggccct ggacgcgggc
     2281 gcttacgggg tgatctgccc catggtcgac acacccgggc aggctgaatc gttcgtcggt
     2341 gcctgccgct atccgccgga cgggtaccgt agctacggcc ccttcagggc ggccatctac
     2401 ggcggcgagg actacaccga gcacgccaac cagaccgtcg tcgctatcgc catgatcgag
     2461 acgcgcgagg ccatcgagaa cctggacggg atcctggccg tcgatggcct cgacggggtc
     2521 ttcgtcggtc cctcggacct tggccagagc ctcggttacg ggcctggcac cgaccgcgat
     2581 gagcccgagg tggtcgaggc gatcgatagg gtggtgtcca ccgcgcgaga ccgcgggctc
     2641 gccgccggca tctttacggg cacgcccgaa cacgcctcgc gcatgatcga gaagggtttc
     2701 aacttcgtca acgtctccac ggacgtacaa ctgctggcgg cggcatcagc cggggtggtg
     2761 acccaactca ggggcgagta agggtatcat ggggagagaa tagccgtttc tcgtgggagg
     2821 agcatcgcgt ggcacagagg gagacgaccg tcggtccaga ggcgggcata cacggcaggc
     2881 cggccgcccg aatcttgaag gcggccaaga gcttctcgtc ccagatctct ctcgtaaagg
     2941 gagaccggga ggtgaacgcc aagagcatca tcaagatcac ggggttcgcc aggaagggcg
     3001 agcaggtgat tgtccgagcc gagggcgacg acgccgagca ggccgtagaa accgtggcgg
     3061 cggagatctc ggccgatgaa gactagcccc gggcggcgaa ggtgaagctg tcaggcgtgg
     3121 gggcatccga aggcgtggcc gttggccccg ccttcgttcc gcggacgggc ggatcagaac
     3181 ccgagagaac cgggattggt gagcgtgagg tagaggccga gatcgggcgg ttcggcgacg
     3241 cggtccgggt tgtggccgcc aggatctccg agaccgcaga agggctacgc gagtcgggga
     3301 acgaggacga ggccggcatc ttcgacttcc atgcggagat ggccgaagac ccggagcttg
     3361 cctccgccgt ggaagagcga gtcaagaacc tgcagagtcc cgaggccgcc gtgctcgccg
     3421 ccggggagga gtacgcggcc gagttcgagg caatcgaaga cgaatacctc tcctcccgcg
     3481 ccgacgacgt gcgtgatgtg gcgcgtcaga tctccgccga gctcatgggc caggcggact
     3541 ctggcttcga ggccctgtac gagccctccg tcgtgcttgc gaagagcctc gcgccctctg
     3601 ataccgcgcg catcccgaag ggcctggcgc tcgccttcgt caccgccgag gggtcaagga
     3661 cctcccatgt ctccatcatg gcccgctcca tgggcatccc cgccgtggtc ggcgtgggag
     3721 acgcactctc gggcgcactg gaagccgaga ccgtcgccgt ggacggcacg gaaggctcgg
     3781 ccatcacgga ccccgatccc gagacggtcg ccaggttcga taagttgtcg caggcactgg
     3841 ccgaggaacg ggtcgcgctc gaccagtaca ggcatgcgga ggcccgcacc gccgacggac
     3901 tgcgcatcga ggtagccgcc aacctcggct cggcctccga ggcggaggag gcgctagagt
     3961 ggggggccga gggcgtcggg ctcttccgca cggagttcct ctttatggag cgcgacgacc
     4021 tgccaacaga agaagaacag tacgacgcct acagtaccgt cgcccgcgct ttcggccaca
     4081 aacccgtcat catccgcacc ctcgacgtgg ggggggacaa gaaccttcca ggggtagacc
     4141 agatccacga ggacaacccc ttcctcggct ggcggggcat caggatgagc ctcgatacgc
     4201 ccgacctgtt caaaccccaa ttgcgcgctc tgctgcgggc cgctgcccac ggcaacgtca
     4261 aggtcatgtt ccctatggtc gtcgaagccg gggagcttct ggcggcgaaa gagatgcttg
     4321 ccgaggcccg cagggaactg gaggagcagg gcgcggacgt cggggagatc gagacagggg
     4381 tgatgataga gacgcccgcg gctgcggtct gcgcggcgga gcttgcctcg gaggcggcct
     4441 tcttctccat cggcaccaac gacctggtcc agtacacgct cgccgcggac aggggcaacg
     4501 agcgactcag gaggctgcaa ggcgccgacc accccgcggt actggccctg atccgtcaga
     4561 cttgtagtgc tgcgcaggag gcgaacatac ccgtgggcgt ttgcggtgaa gcggccggcg
     4621 acccgcaact gatccccaac cttttagacc tgggcgtaac agagctaagc atggccgccc
     4681 cctctatacc gaaggccaag aaggtggtgt cggagtggaa acggacgtag tggcgggtct
     4741 gaaccaagcc tccccaaccg ggcgcgaggc gatccaatga aggcccgcat cttccccgat
     4801 tacgaggaca tgagcgcggc agccgccgac gccgtcatcc gcagggtaag cgagaagcca
     4861 acctccgtct tcctgctgcc cacggggacg acgccgctgg ggatgtaccg gcgcctcgtg
     4921 gacgtgtacc ggctgggtgg ggtctccttc gccggggcgg ctttcttcaa cctggacgag
     4981 tacctcgggc tggtgccgga ccatccggcc agctaccacg tgtacatgaa ggatcacttc
     5041 tacggcctca tagacgccga cccggcacgc atcttcgtcc cagacggcgc cgccccggac
     5101 cccgtagcgg agtgcgagcg gtacgaggcg gccatagagg cgttcggcgg cgtggacatg
     5161 tgcgtgcttg ggatcggacg caacggccac atcggtttca acgagccagg cgcgtcgttc
     5221 cactcgcgga cgcgggtcgt gacgctttcg gagagcaccc gcctcgtcaa ctccgcagat
     5281 ttcgaggcca accgcgcgcc cgagcgggcc attacggtcg gcatggcgac catcgccgaa
     5341 tcccgggagg tcctgctgct agcctccggc accaacaagg ccagcgcggt cgcggcggcg
     5401 ctcgaagggg aggtctcgga ggatatgccg gcctcgatgc ttggagaaca cgctcgtgcc
     5461 acgatgttcc tcgacgagga cgcggcctcg ggcctcggcc agggcgcagc aggttagcgg
     5521 cacgttgacg cggaagtggc gctccagtag catcggctca ggtgggttct tgtagggagg
     5581 ggatggaagc cctgaaccgt caacggtcct cgcagagtcg atggcagggt ccccgtgtgc
     5641 ccgtggtcgc ggggcccgga tgagggtcgg actgctcgga gccggccgca tcggggtgct
     5701 ccacgctggc ctcctcgcct gcgaccccag ggttaaagag ttcctcgtgg gggacgccga
     5761 cgagcggcgc gccgagagca ttgccgcgga ggtgggggga aggtcggggt ccatcgagga
     5821 tatcctccgg tcggggcccg attgcgtggt catcgccgcc tccacccagg cgcacgcgcc
     5881 cctgatacta gcctgcgtcg aggccggcat accggcgttt tgcgagaagc ctatcgcgct
     5941 cggctacagc gacacggtcg aggtcgtgga ggccgtcgag tccagcggcg ccgtgctcca
     6001 gatcggcttc cagaggcgct tcgacgccgg gtacgccgaa gcgcggcgcc tcgtggagag
     6061 cggggagctt ggaaccatct actccgtgag gcttgccacc cacgaccccg aaccaccacc
     6121 cgaggagtac atcgcgggat ccggcggtat cttccgtgac cttcacatcc acgacttcga
     6181 cgtactgcgc tggctgacgg gcgccgaggc tcgagaggta tacgccgctg gcggcgtacg
     6241 cggcttcggc ttcttcggcg agcacggcga cgtggatacc tcggcggccc tcatcacgat
     6301 gcaggacggc tcggtggcgg tactgaccgg tggccgtcac gacccccttg gctacgacgt
     6361 gagggcggag atcttcggct ccagagacag cgtcgccgtt ggcgtgaacg gccgcacacc
     6421 cctgcgatcc gtcgagccgg gcgtacagcc ctcggggaag gtcgcgtatc cggacttcca
     6481 gaaccgcttc ctcgacgctt acagagccga gctggagcac ttcctctccc ttgcccgtgg
     6541 cgaggccgag aaccggtgca ccgcccgcga cgccctcgag gcgcttaaga tagctatggc
     6601 cgccgacctc tcgctcgccg aacaccgccc cgtcatggtg tccgaggtcg gatgaggcgg
     6661 tcggaaaagg ggcgcgaacc attagagccg cagtattcgt cacgcaggcg aggaagggag
     6721 taacttgtgg gaacatcggt gccaaggggg ctatcgcgta gggacttcct gaaaactagc
     6781 ggtgcggggc tcgccggggc cgttttgctc ggggccgcgg gctgcggtgg ggccgagcag
     6841 ggcggtgggg tgaccgagct gtactacacg gcatccccgg acgagtcggg gactacccag
     6901 aagcttatcg acaagttcaa ccagcagaac aagggcacct acaaggtcat cttccgcgag
     6961 ggcaactcgg acacgggcca acgcctggac aagctaagga cccagttcca ggccggcggg
     7021 gaggaactag acgtgatcct cggtgacgtc atctggaccg ccgagctcgc cgcgaacggg
     7081 tgggttacag atctaacgga ccggttcccg cagagcaccc agaaggagta cctgcccggt
     7141 tcggtcgaag ccataatcta cgaggacaag ccctacgcca tgccatggta cacggacacg
     7201 ggtctcctct actaccgtaa agatctgcta gagcagagcg gtttcgacgc ccctccaaaa
     7261 tcctgggacg agctgaaaac gatggccctc aaggtcatgg atcagtccgg gaccaagttc
     7321 ggtttcgtgt ttcagggtgc caggtacgag ggcggggtgt gcgacggctg cgagttcatc
     7381 tggggccacg gcggaaacgt actggatccc gaggacccga acaaggtcat cgtggacagt
     7441 cccgaggcca tagcaggcct cgccacggag cgcagcatga taaccgacgg tgtctcgccc
     7501 caggcggtga cggtctacaa ggaagacgag tcggccgggg ccttcctgaa cggcgacgcc
     7561 gtcttccttc gcaactggcc ctacgtgtac gccctgatcg gcacctccga ctatccgaag
     7621 ctggacaccg accaggtcgg cgtttccgag ctaccgtccg gcgacggtaa gccaggcaac
     7681 ggaacggtgg gcgaccagcc actgtacatc agctccagct ccaagaaccc ggacgccgcg
     7741 tggaagttca tcgaattcct ctcctcgccc gagcagcaga aattgagggc gatcgagggc
     7801 tcgtacctgc cgaccaggtc cgacctctac gacgacccgg agatcca
//
LOCUS       DBNGVD010000176         4605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4956253, whole genome shotgun sequence.
ACCESSION   DBNGVD010000176 DBNGVD010000000
VERSION     DBNGVD010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4956253"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="AC16CM_04930"
     CDS             <1..1461
                     /locus_tag="AC16CM_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3139205.1"
                     /translation="LSELVAGFALVAVVLTVAGLTSGFVERAPISFPIIFLGLGLLLG
                     RLDILHLGPESALLETVATLTLALVLCLEAVRIGNIGTGGAGFVPILSLGPGTVVIVA
                     VIAVGSYLLLDIAVIEALLLGTILASTDPVVLRDVVRESRIPASVRQALNIEAGTNDI
                     VVLPTLLILIALANDEASGAGGWALLIAKVLVLGPVIGFVIGAGAAWLMTQADRRYAV
                     NQVYQSLYGIGIVLLAYASATALGGDGFLAAFAAGLAVAVLNFDLCQCFLDYGETTSE
                     MAMLLSFILFGVVIYDLFAEVPMLASLILAAVVIFIARPLAIGLVLRKAAVSNAARAF
                     IGWFGPRGLNSLLLALLVVEAGVAGSEFLLGVVGVVVTVSVVIHGASATPLSRRYGEA
                     VMRETHEEERDDAAGIFEGAASESIRISASQLSGMLGERNPPVVLDVRTRSQYEQDHS
                     RIPGAVRVAPDEIDEWARERERGSQIVGQRVVAYCT"
     gene            complement(1668..2645)
                     /locus_tag="AC16CM_04935"
     CDS             complement(1668..2645)
                     /locus_tag="AC16CM_04935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014642406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIR3139206.1"
                     /translation="MIPAKLRPGDEVRVVSPAVSLGFIPEEQRTMARERFEALGFRVS
                     FSSNVEVLDRFDSSPVEVRVSDLHEAFSDPEVRGVFTTLGGYNSNQLLDYLDYELIGA
                     NPKVLCGFSDITALATAIHARTGLVTYSGPHFTTFAMKRGIEYTTEFLERCLMREEPF
                     EVPPADHWSDDPWYEDQEARDFVPNPGYEVIHEGEAGGRLLGGNLGTLALLFGTPYMP
                     DLEGAVLLLEDDEEESAFTFDRHLQSLIHQPGFTGVGGLVIGRFQRASEMDLDTLKAI
                     IGSKRELDRLPVVANASFGHTSPQFTFPIGGYGTLRAENGRVELRIDEH"
     gene            complement(2658..3686)
                     /locus_tag="AC16CM_04940"
     CDS             complement(2658..3686)
                     /locus_tag="AC16CM_04940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3139207.1"
                     /translation="MKSGTHTFLPTSRVHFGEDALGRLQEEARPYGRAFVLTGRSLNE
                     KTDLVRNVEALLGGRHAGTFAGIGQHTPGGTVEEAASHAEDAAADLLVAVGGGSVIDG
                     TKAVARQLGYPEQVAVPTTLSGAEWAQRVGVTDESEGLKKGFADPRTVPPVVILDPAA
                     TVYTPEPLWLSTGIRAIDHAVEGYLYGGDHPITDVTGLEGARRLMEYLPRSKRSPEDL
                     EARLELQLAAWLAYFGPMNTPMGLSHELGRRIGASYDVPHGITSCIVLAASLEVLGAR
                     IPGERWSGLSEALGGEPPERIAALVRDLELPASLREVGVPEEELESIAAEYGDRQVDV
                     SDIFRAAY"
     gene            complement(3722..>4605)
                     /locus_tag="AC16CM_04945"
     CDS             complement(3722..>4605)
                     /locus_tag="AC16CM_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR3139208.1"
                     /translation="TKRVILVSGATGQQGGTVARNLLKRGFAVRALTRDTEKAAAREL
                     ADLGAEVVGGDLEDRESVDRALEGVYGVFSVQQFWGIGAEGEIRQGVLLADAARAAGV
                     QHLVYSSVGSAHRETGIPHFDSKWEVEEHVRASGVPYTVLRPVFFMQNWEFMREPVLG
                     GVLPQPLDPDKPFQMIDSDDIGVFAADAFDDPETWVGREVDIAGDELTMPEIAGVFSR
                     VIGRNVDYVQVPWDAFEEQMGEEYAVMYRWFNDHGYEANIAALRDEYPELSSFEQYLR
                     AHGWENAEVPSGTGAGS"
BASE COUNT          764 a         1551 c         1435 g          855 t
ORIGIN      
        1 ttgtctgagt tggtcgcggg cttcgcgctc gtcgccgtgg tcttgaccgt cgcgggcctc
       61 acctccggct tcgtagagcg ggcgccgatc tcgttcccga tcatctttct cggcctaggt
      121 ttgttgctgg gccgcctaga tatcctgcac ctgggccccg agagcgcgct gctggagacc
      181 gtggcaacac tgacgctcgc cctggttctg tgcctggaag ccgtgcgaat aggcaacatc
      241 ggcacgggcg gcgccgggtt cgtacccata ctgtcgcttg gaccggggac ggtcgtgatc
      301 gtcgccgtta tcgccgttgg ttcgtacctc ctgctcgaca tcgcggtgat cgaagcgttg
      361 cttctcggca ccatcctggc ctctacggac cccgtagtgc tgcgcgacgt ggtcagagaa
      421 agtcgcatac ccgcctccgt gaggcaggcc ctcaacatcg aggccggcac caacgacatc
      481 gtcgtgctgc ccactttgct catcctgatc gccctcgcca acgacgaggc ttctggcgcg
      541 ggcgggtggg cactgctgat cgcaaaggta ctcgtgctcg ggcctgtgat tggcttcgtc
      601 atcggtgcgg gcgcggcgtg gctgatgacc caggcggacc gacgctacgc ggttaaccag
      661 gtctaccaat ctctctacgg catcgggatc gtacttctcg cctacgcgag cgcaacggca
      721 ctaggcggtg atggcttcct cgccgccttc gctgccggcc tggcggtagc ggtgctcaac
      781 ttcgacctgt gccagtgttt tctggactac ggtgagacca cgagcgagat ggcgatgctg
      841 ctctccttca tcctgtttgg cgtcgtgatc tacgacctgt tcgcggaggt gcccatgctg
      901 gcctcgctca tcctggccgc ggtggtgatc ttcatcgccc gcccgctggc gatagggctg
      961 gtgttgcgca aggccgccgt gagcaacgcg gcgcgggcgt ttatcgggtg gttcggcccg
     1021 cgcggcctga attctttgct gctggcgctc ctcgtcgtcg aggccggtgt tgctggctcc
     1081 gaattcttgc ttggcgtcgt tggcgtggta gtcaccgtct ccgtcgtgat ccacggggcc
     1141 tcggccacac ccctctcgcg ccgctacggc gaggcggtca tgagggagac ccacgaggag
     1201 gagagggacg atgccgccgg catcttcgag ggtgccgcga gcgagtcaat caggataagc
     1261 gcctcacaac tctccggaat gctgggggag cggaatccgc ccgtcgtact cgacgtccgc
     1321 acccgctcgc agtacgagca ggatcacagc cgtatccccg gcgccgtgag ggtcgctcct
     1381 gacgagatag acgaatgggc acgagaacgg gagcgcggga gtcaaatagt agggcaacgc
     1441 gtcgtagcct actgcacctg accggacgag gctaccagcg cccgggtggc gcggaaacta
     1501 aacaaaatgg gttacgaggc aaccgccctg gaaggcggct tcgacgtctg gaaggcacaa
     1561 tacccgacag agagcccgcg acctgaaacg gtagcgtaag cttctcactg ttcttagccc
     1621 gaaaccagag gtaggggcgc ttcgcgaagc gcccctaccg aagcgctcta atgctcgtcg
     1681 atccttaact ccacgcgacc gttctcggct cgtaacgtac catagccccc gatcgggaac
     1741 gtgaattgtg gcgaggtgtg gccgaagctg gcattggcga cgacgggaag gcggtcgagt
     1801 tcccgcttgc ttccgatgat ggccttcagg gtgtcgaggt ccatttccga tgctctctgg
     1861 aaccttccga tcaccagtcc ccccacgccc gtgaacccgg gttgatggat gagcgattgc
     1921 aggtgcctgt cgaacgtaaa ggcgctttcc tcctcatcat cctctagcag aagaactgcc
     1981 ccctccaggt cgggcatgta cggggtgccg aacaggaggg ccagggtgcc aaggtttccc
     2041 ccgagcaatc tcccccccgc ctcgccctcg tggatcacct cgtagccggg gttgggcacg
     2101 aagtcgcggg cttcctgatc ctcgtaccac gggtcgtcgc tccagtggtc ggcgggcggc
     2161 acctcgaagg gttcctcgcg catcaggcag cgttccagaa actcggtggt gtactcgatg
     2221 ccacgcttca tggcgaaggt ggtgaaatgg ggtcccgagt aggttacgag gcccgtcctc
     2281 gcgtggatcg cggtggccag cgctgttatg tccgagaagc cgcagaggac ctttgggttg
     2341 gccccgatca gctcgtagtc gaggtaatcg agcaactggt tgctgttgta gccgccaagg
     2401 gtggtaaaga ctcccctgac ctccgggtcc gagaacgcct cgtgcaggtc agaaaccctc
     2461 acctccacgg gcgaagaatc gaagcggtcc aacacctcga cgttgctgga gaacgaaacg
     2521 cgaaacccga gcgcctcgaa ccgctccctg gccatggtcc tctgctcctc cgggatgaac
     2581 ccgaggctga ccgcgggtga gactaccctg acctcgtcgc ccggccggag cttcgcgggg
     2641 atcacgggca aaccagccta gtaggcagcc ctgaaaatgt ccgacacgtc aacctgcctg
     2701 tccccgtact ccgcggcaat ggactcgagc tcctcctcgg gcacgccgac ctcgcgcagg
     2761 ctggccggca attcgaggtc ccgtacgagc gcggctatcc gctccggcgg ctccccgccg
     2821 agcgcctcag agagcccgct ccagcgctca cccggtatcc gcgctcccag cacctccagg
     2881 ctcgctgcga gaacgatgca agaggttatg ccgtggggaa catcatagct ggccccgatc
     2941 ctgcgcccca gctcgtgcga gagccccatg ggcgtgttca tcggcccgaa gtacgcgagc
     3001 caggcggcga gctgcaactc gaggcgcgct tccaggtctt ccggcgaacg tttcgaacgt
     3061 ggcaagtact ccatgaggcg gcgcgcgccc tccaggccgg tcacgtcggt tatggggtgg
     3121 tcgccgccgt agaggtatcc ctcgaccgcg tgatctatgg ccctgatacc ggtcgagagc
     3181 cagaggggct cgggcgtgta gacggtcgcg gcgggatcga gtatcacgac gggcggcacc
     3241 gttctcggat cggcgaagcc cttcttgagc ccttcgctct cgtctgtaac acccaccctc
     3301 tgggcccatt cagccccgga gagggtggtc ggtaccgcca cctgctcggg gtagccgagt
     3361 tgcctggcga cggccttggt gccatcgatg acgctgccgc cgccgacggc gacgagcagg
     3421 tcggctgcgg catcctcagc gtgcgaggcg gcctcctcga cggtcccacc cggggtgtgc
     3481 tgcccgatcc cggcgaaggt tcccgcgtgc ctgccgccca gcaaggcctc gacgttccgg
     3541 acgagatccg tcttctcgtt caggctgcgg ccggtcagga cgaaggcccg accgtagggc
     3601 cgggcctcct cttgcaatct cccgagggcg tcctcgccaa agtgcacgcg ggaggtcggg
     3661 agaaaggtgt gtgttccgct cttcacaccc ctatgttacc gtcgttcgcg gccccgggcg
     3721 actagctgcc agcgcccgtc ccggagggga cctcggcgtt ctcccatccg tgggcccgga
     3781 gatactgctc gaaggaggac agctcggggt actcgtccct gagggctgca atgttcgcct
     3841 catagccgtg gtcgttgaac caccggtaca ttacggcgta ctcctccccc atctgctcct
     3901 cgaacgcgtc ccaggggacc tgcacgtagt ccacgtttcg cccgatcaca cgcgagaaca
     3961 cgccggcgat ctccggcatg gtcagctcat caccggcgat gtctacctcg cgcccgaccc
     4021 aggtctccgg atcgtcgaag gcatcggccg cgaatacccc gatgtcatcg gagtcgatca
     4081 tctgaaaggg cttgtccgga tccaacggct ggggaagcac cccgccgagg acgggctcgc
     4141 gcatgaactc ccagttctgc atgaagaaga cgggacgcaa caccgtatac ggtacgccgc
     4201 tagcccgcac gtgctcctcg acctcccact tgctgtcgaa gtgcgggatg cccgtctccc
     4261 tgtgcgcgct tcccacggag ctgtagacga gatgctggac accggccgcc ctggccgcgt
     4321 ccgcgagcaa tacaccctga cgaatctcgc cctcagcacc tatgccccag aactgctgca
     4381 ccgagaacac cccgtagacg ccttctaggg cacggtctac cgattcccgg tcctcgagat
     4441 cgccgccaac cacctcggcc cccaggtcag ccaactccct cgcagccgct ttctcggtat
     4501 cacgggtcaa cgcccgtaca gcgaatcccc tcttcaacag gttacgggcc acagtcccac
     4561 cctgctgccc ggtagccccg ctcaccagga tcacacgctt cgttt
//
LOCUS       DBNGVD010000177         3248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3862779, whole genome shotgun sequence.
ACCESSION   DBNGVD010000177 DBNGVD010000000
VERSION     DBNGVD010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3862779"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC16CM_04950"
     CDS             complement(<1..543)
                     /locus_tag="AC16CM_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3139209.1"
                     /translation="MKTDSRPILLLSTVAFLFCSVLAGTGLFRPLDEVVLALAQRGNS
                     ALVDGIGLLASLLGGVEFVAVAALTLAAGLVVRGQRRLAARLLAAFVITGIIELVFKM
                     VVPQVPVPDEVLRGPDPSLFDVSTPYPYPSGHMLRAVLLFGAVYILWPNGLLRLAIVV
                     FLVVSAASRLYLGTHWPSDML"
     gene            complement(656..1540)
                     /locus_tag="AC16CM_04955"
     CDS             complement(656..1540)
                     /locus_tag="AC16CM_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3139210.1"
                     /translation="MKANLDRQRDPKKIERESETPELLAKYLAHIGQGNLLTHKEEID
                     LSLRAKAGDLRARQRLIEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDP
                     DRGFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKVSRAISELSLEIEREPT
                     EEEVAKRLDWDPDEVRLTMRAMPDATSLDQPVSSEETASQLGDFIEDDKVSDTPDTVM
                     REMETEHLKEAIERLPERARYVLVRRYGLDDREPATLAELGDELDISRERVRQLQREA
                     ERILKGGEYGRVLRDAVA"
     gene            1767..2699
                     /locus_tag="AC16CM_04960"
     CDS             1767..2699
                     /locus_tag="AC16CM_04960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3139211.1"
                     /translation="MMDSTSRIPDRIDEDVAGIVACWQERRGGDGDDGANGRMLAGTE
                     ELVSVFVGFLRSPETVATYSRGGTTRALVERIAGYQHELGRDAVGVIEDFTVLRRCIW
                     DSVESGVELSELQGAEVSEFFGKLMQASDWVTETGLEAFDVIVQREMEEALGRAAATD
                     LVTGLPDRDQLNRLLLPQAIDEHERFSVAVFDIAAFTETVASDGVKRARKSLRTLAQA
                     VQETSPKDSIFARFGDDEVCVLLVDQTGEDAFGVAERVLELLAGQEAGFEVDVGIAEY
                     PAHGQAAGELMAETLKALDTAKRVGGGGIVTAHK"
     gene            2713..3033
                     /locus_tag="AC16CM_04965"
     CDS             2713..3033
                     /locus_tag="AC16CM_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3139212.1"
                     /translation="MIAIFNSLPVREGAADDIVDRFAESRGDVQDFPGFVSMEVLKSD
                     QEDEVLVITRWRDREAFEAWVHSDSFRRAHGRGGTGGLLTGHPTMSSYRVAVERVPGA
                     PRTA"
     gene            complement(3037..3174)
                     /locus_tag="AC16CM_04970"
     CDS             complement(3037..3174)
                     /locus_tag="AC16CM_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139213.1"
                     /translation="MRERLAFGILIVLLIFLFAFTFAVLGKGEYEPGAQAPLQAPSHL
                     A"
BASE COUNT          602 a         1026 c         1027 g          593 t
ORIGIN      
        1 cagcatgtcg gagggccagt gggttcccag gtagagcctg ctcgccgccg agaccacgag
       61 gaacaccacg atcgccagcc tgagcaggcc gttcggccag aggatgtaga ccgcgccaaa
      121 gagtaggacg gcgcggagca tgtgaccgct gggatagggg tagggcgtgc ttacatcgaa
      181 cagggagggg tcggggcccc gaagcacctc gtccggcacg ggtacctggg gcaccaccat
      241 cttgaaaacg agctcgatga tgccggtgat cacgaacgcc gcgagaagcc gtgccgccag
      301 acgcctctgt ccgcgtacga caaggcccgc ggcgagggtg agagcggcca ccgcgacgaa
      361 ctccaccccc cccagcaagg aggcgagtag cccgatcccg tccaccagcg ctgagttacc
      421 ccgctgggct aacgcgagca ccacctcgtc cagggggcga aacagaccgg taccggccaa
      481 caccgagcaa aacaggaaag ccaccgtcga gagcagcaga attggccgac tgtcggtctt
      541 cacgccctca cagactatca gggtggaaac accagagcgc cgggcttagt gcccgacgct
      601 cgtagatcag cgagcccgaa cgcctcgaag atcgcggcgc tggggccgaa tggtcctatg
      661 ccaccgcgtc gcgcaggacg cggccgtact cgccgccttt gaggatgcgc tccgcctccc
      721 tctgcaactg cctgacccgc tcgcgcgaga tatcgagttc gtcaccgagc tcggcgaggg
      781 tcgccggctc gcggtcgtcg agaccgtagc gcctcacgag cacgtagcga gccctctctg
      841 gcaaccgttc gatggcttcc ttcaggtgct ccgtctccat ctcccgcatc acggtgtcag
      901 gtgtgtcgga gaccttgtcg tcctcgatga agtcaccgag ctgggaagcg gtctcctcgc
      961 ttgataccgg ctgatccagg ctcgtcgcgt cgggcatagc gcgcatggtg agacgcacct
     1021 cgtcggggtc ccagtcgagc cgcttggcga cttcttcctc tgtaggttcg cgctctatct
     1081 ccagggacag ctcgcttatc gccctgctta ccttgcggat cttctccgtc atgtgcacgg
     1141 gcaccctgat ggtcctgccc ttgtcggcga ccgccctctg cactgcctga cggatccacc
     1201 acgtcgcgta tgtcgagaaa cggaatcctc ggtcggggtc gaatttttcg acggccttca
     1261 tcaggccgat gttgccctcc tggatcaggt cctcgaacgg cagaccgtag ccgcggtact
     1321 tcttggccac gctgaccacg agcctcaggt tcttctcgat gagcctctgc ctggcccgca
     1381 gatccccggc cttggccctc aaagagaggt cgatctcctc cttgtgggtg aggaggtttc
     1441 cctggccgat gtgggcaaga tacttggcaa gcagctcggg ggtctcggat tcccgttcga
     1501 ttttcttggg gtccctttgc ctgtccaaat tagccttcaa gataccctcc tcgctaaatg
     1561 ccaatcctac cggaaatcag ttttgaaaac tactattgat ttaccgcgaa gtttaagtcc
     1621 ctaaacgttt cgggacctcc ctcttcgccg gttctcgtac atgtttgtca ccttactgat
     1681 acatacgtac gcagaccaga aatggttgca tcctgggtgc gccctcccgc acccgccggt
     1741 gagatgggct ggcggggtat actctgatga tggatagcac ctcccgaatc ccggaccgca
     1801 tagacgagga tgtcgccggc atagtggctt gctggcagga gcgaaggggc ggagatggcg
     1861 atgacggggc caacggccgg atgttggcag gcacggagga gctggtatcg gtcttcgtcg
     1921 gcttcctgcg cagtcccgag accgtcgcga cctacagcag gggcgggacg acccgcgccc
     1981 tcgtagagag aatcgccggt taccagcacg agctggggcg ggacgccgtg ggcgttatag
     2041 aggacttcac cgtactcagg cggtgcatct gggactcggt cgagtcgggc gtagagctct
     2101 cggagctgca gggcgccgag gtatcggagt tcttcggaaa gctcatgcag gcgtccgact
     2161 gggtcaccga gaccggactc gaggccttcg atgtcatcgt gcagcgggag atggaggagg
     2221 ccctcgggcg ggccgccgcc acggacctcg tgaccgggct tcccgacagg gaccagctca
     2281 accgcctgct actgccgcag gccatcgacg agcacgagcg gttctccgta gccgtcttcg
     2341 acatcgctgc tttcaccgag accgtggcgt cggacggggt caaacgcgcc agaaagtctc
     2401 tgcgcacgct cgcacaggcg gtgcaggaga cctccccgaa ggattcgatc ttcgctcgat
     2461 tcggcgacga cgaggtatgc gtcctacttg tcgatcagac cggtgaggat gctttcgggg
     2521 tggctgagcg cgtgctcgaa ctcctcgccg ggcaggaagc cggcttcgag gtcgacgtcg
     2581 gcatcgccga gtatccggcc cacgggcagg cggcagggga gttgatggcc gagacgctca
     2641 aagccctcga cactgccaaa cgcgtcggag gcggcggcat cgtaaccgcc cacaagtagt
     2701 caggagatag gtgtgatcgc gatcttcaac agccttccgg taagagaagg cgcggcggac
     2761 gatatcgtgg accgtttcgc agagagccgc ggtgacgttc aggacttccc ggggttcgtc
     2821 tccatggagg tactgaagtc cgaccaggag gatgaggtcc tcgtcattac ccgctggcgc
     2881 gatcgagaag ccttcgaggc ctgggtccac agcgactcgt ttcgccgtgc ccacgggcgt
     2941 gggggtacag gcggtctgct tacgggccat cccacgatga gctcttacag agttgcggtt
     3001 gagcgcgtcc ccggtgcccc ccgaacggcc tgaagatcag gccaggtgcg agggagcttg
     3061 cagcggggcc tgcgcacccg gctcgtactc gcccttaccg agcacagcaa acgtgaaagc
     3121 aaacaggaag atcagcaaca cgatcaaaat cccgaacgct agacgttccc gcacctggtc
     3181 tcctctctcg aatacgaacc tgcgcgcatt ctaacctaat cggcggggct cgtgtttgca
     3241 gtcccgcg
//
LOCUS       DBNGVD010000178         3775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3681426, whole genome shotgun sequence.
ACCESSION   DBNGVD010000178 DBNGVD010000000
VERSION     DBNGVD010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3681426"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16CM_04975"
     CDS             <1..182
                     /locus_tag="AC16CM_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139214.1"
                     /translation="PYSFASGVLLKSNMPATIAETVFITNDREAQLLSNGTGTRQHQI
                     AQALKTGIERYLATQ"
     gene            396..872
                     /locus_tag="AC16CM_04980"
     CDS             396..872
                     /locus_tag="AC16CM_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR3139215.1"
                     /translation="MDDKRKRYVEEFGLLFGQFGLSRMLGRVLGVLMISDPPERSAGE
                     LAEALGASRGSISQTTRSLIQMGLVERRARPGERRDYFCVKPGAWGGLMRREMDALGE
                     FRETAERGLAMLGSGDPEVRRNLEEMRDFYAYWEKEMPAVLERWEKEGSHGTRDTD"
     gene            850..1755
                     /locus_tag="AC16CM_04985"
     CDS             850..1755
                     /locus_tag="AC16CM_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139216.1"
                     /translation="MGLAIQTEKLTKSYGRKARGIEEVDLAVEEGEVFGFLGPNGAGK
                     TTTVRTLLGFLRPTSGRGEVFGLDIKKDSVEVRATVGNLPGEFALEDKLTGERLLRYF
                     ARLRGVRDLGYAYELAERFGAELHRPMRRLSRGNKQKIGLVQAMFHRPPLLMLDEPTG
                     GLDPLVQEEFLDIVEEVKAEGRTVFISSHNLAEVERICDRVGIIRGGRLVAVETTDTL
                     IDKSFRHVVLTFAEEVDPKPFEALPGVRDLRAGGNRLAFTLYDDLDEMVKLAAGHHLL
                     NMEYERPSLEEVFLAYYERENGESR"
     gene            1752..2591
                     /locus_tag="AC16CM_04990"
     CDS             1752..2591
                     /locus_tag="AC16CM_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3139217.1"
                     /translation="MSTVAEQKRPQRTAPLGTLLAFVSHTVRMQLRGMIIWGAALGLY
                     SAAMVASFTAIEGSAEQLNQLMDAYPKGMLEAFGVTDLGDPANYLHSQFFGLAPLALS
                     FFPIMAFSGAIAGAEERGTIDVLLGNPLPRWQLVVGSFLATALSLLGICAIVGILTWG
                     TAVLVDVDLSWREIMDGVLNLWPITIAFGSLALLCSSLFHRKALATSVPAFLLFGMYL
                     LDAIGRASEDLEDWRPASAFYYYGSAVENGIDWTHAGAITLVALALTLLATLAFRRRD
                     IYT"
     gene            2841..>3775
                     /locus_tag="AC16CM_04995"
     CDS             2841..>3775
                     /locus_tag="AC16CM_04995"
                     /inference="COORDINATES: protein motif:HMM:NF017485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HIR3139218.1"
                     /translation="MEHGTLVRARGLGVLRGVLSSPVVRDFLELLGLLEGRPDPGTLA
                     DVFGRLWEELAFEEERLLPDAWQSYLVGRMLDDENAFSLGAERGTVSPSVWRQAGRDL
                     ITLREMFAWDAETLLGGVEAAVPALTDMWVAWSDPAPEEDSPRHNIARKLSAAEDWEA
                     CVPLLADHFARHGAGPFGRHRVFQWRGGGLRAVTLPDPVRLEGLISYETEREPLVLNT
                     ERFLSGLPAHHALLYGPPGTGKSSTVKAVLNEYAGAGLRLVEVRKEDLADLGGVLEDL
                     RGRGPRFVVYVDDLSFEEHEVEYKALKALLEGSVE"
BASE COUNT          671 a         1156 c         1267 g          681 t
ORIGIN      
        1 ccccatactc cttcgcctcg ggcgtcctcc tgaagagcaa catgccggcg accatagccg
       61 agaccgtctt catcacgaac gaccgggaag cacaactcct ctccaacggc acaggcaccc
      121 gccagcacca gatagcccag gccctgaaaa caggcatcga acggtacctc gccacgcaat
      181 aacagggaaa ggtttcaggc ctcagcagga tgcgtgtcac gattttcgcg aaagcaccgt
      241 caacctcgta ggctcccacc tgcagcgggt tgcgtgcaag ccttcgacca gacgccaaaa
      301 agctgacagc tacctttcaa acctttcaca aatcattgaa acttgctagc tacgggagta
      361 tcctgtgggt aattcgaggc gttcacggag ctattttgga cgataagcgg aagcggtacg
      421 tcgaggagtt cgggctactt ttcgggcagt tcgggctttc gcggatgttg gggcgggtgt
      481 tgggggtgtt gatgatctcc gatccgccgg agcgttcggc tggcgagttg gcggaggctc
      541 tgggggctag caggggttcg atcagccaga cgacgaggtc gctcatccag atggggcttg
      601 tcgagcgtcg ggcgaggcct ggtgagcggc gggattactt ttgcgtaaag ccgggggcgt
      661 ggggtggtct catgcgccgc gagatggatg cattgggcga gtttcgggag acggcggagc
      721 ggggtctcgc gatgcttggc tccggcgatc ctgaggtcag gcgcaacctc gaggagatgc
      781 gcgacttcta cgcctactgg gagaaggaga tgcccgccgt gctggagcgg tgggagaaag
      841 aaggttcaca tgggactcgc gatacagact gagaagctaa ccaagtccta cgggcgaaag
      901 gcgaggggca tagaggaggt agacctcgcg gtcgaggagg gtgaggtatt cggcttcctc
      961 gggccaaacg gggctgggaa gaccaccacg gtaagaacgc tcctagggtt cctgcggccc
     1021 acgagcgggc gtggtgaggt cttcgggctg gacatcaaga aggacagcgt cgaggtcagg
     1081 gccaccgtcg gcaacctgcc cggggagttc gccctggagg acaagttgac gggggagagg
     1141 ttgctccggt acttcgccag gttgcgcggg gtcagggacc tcgggtacgc gtacgagctg
     1201 gcggagcggt ttggggccga gttgcaccgt cccatgcgca ggctctcgcg ggggaacaag
     1261 cagaagatcg ggctggtcca ggccatgttc cacaggcccc cgctcctgat gcttgacgag
     1321 ccgaccggcg ggctcgaccc gctcgtgcag gaggagtttc tggacatcgt cgaggaggtg
     1381 aaggcagaag ggcgcaccgt gttcatctcc tcacacaacc tcgccgaggt cgagaggatc
     1441 tgcgaccggg tcgggatcat ccgcggcggc aggctcgtcg cggttgagac cacggacacc
     1501 ctcatagaca agtccttccg gcacgtcgtc ctgacctttg cggaggaagt cgacccgaag
     1561 cccttcgagg ccctccccgg cgtcagggac ctcagggccg gcgggaacag gctggccttc
     1621 acgctctacg acgacctcga tgagatggtc aagctcgcgg ccggccacca cctcctcaac
     1681 atggagtacg agcgtcccag cctcgaagag gtctttctgg cctactacga gcgggagaac
     1741 ggagaatcac gatgagcacg gtcgcggagc agaagaggcc acagaggacg gcgcccctgg
     1801 ggacgctact cgcgttcgtc tcccacaccg tcaggatgca actacggggc atgatcatct
     1861 ggggggccgc gctcgggctg tatagcgcgg ccatggtcgc ctcgttcacg gccatcgagg
     1921 gaagcgccga gcagctcaac cagctcatgg acgcctaccc gaaaggtatg ctcgaggcct
     1981 tcggcgtcac cgacctcggc gaccccgcca actacctgca ctcgcagttc ttcggcctcg
     2041 cgccgcttgc cctgtcgttc tttcccatca tggcgttctc gggcgccata gcgggcgccg
     2101 aggagcgcgg caccatagac gtgctgctcg gcaaccccct gccgcgctgg cagctcgtcg
     2161 tcggcagctt cctcgccacc gccctctccc tgctcggcat ctgcgccatc gtcgggatcc
     2221 tgacctgggg tacggccgtg ctggtagatg tggacctctc ctggcgggag atcatggacg
     2281 gagtgctcaa cctctggcca ataacaatag ccttcggctc acttgccctc ctgtgctcct
     2341 cgctcttcca ccgcaaggcc ctggccacct ccgttcccgc cttcctgctc ttcggcatgt
     2401 acctcctcga cgccataggc cgcgcctccg aagacctcga agactggcgg cctgcctccg
     2461 ccttctacta ttacggatcc gccgtagaga acggtatcga ctggacgcac gccggcgcca
     2521 taaccctggt agcgctagcc ctcacgctcc tggcaacgct cgccttccga aggcgcgata
     2581 tctatacgta gcacgcgtag gctgacgcct acgcttgtca gcacgctccc tttggtcgct
     2641 tgtcagccag ggctaggcct gcacctttcc gcacgggtgt atcgatgctc aggaaagccc
     2701 tatgtcgaag ctgccgttac ggagagagac gactggctga cagcgaggcg aagccgaagc
     2761 gtgctgaaag cgagagcctt caggctcgaa gcgttgccga caagcggagg cgaagccgaa
     2821 gcgtgctact atgctccttc atggagcatg gtacgctggt cagggcgcgt gggttgggag
     2881 ttttgcgggg ggtcttgagt tcgcccgtcg tgcgcgattt tctggagctt ctggggttgc
     2941 tcgaggggag gccggatccc ggcaccctgg cagacgtttt cgggcggttg tgggaggagt
     3001 tggccttcga ggaggagcgt ctgcttcccg atgcctggca gtcctatctc gtgggacgga
     3061 tgctcgatga tgagaacgct tttagcctgg gggccgagag ggggacggta agtccttcgg
     3121 tgtggcggca ggcggggcgg gacctgataa cgttgcgaga gatgttcgcg tgggacgccg
     3181 agacgttgct cggcggggtt gaggcggccg tgccagccct caccgacatg tgggttgcgt
     3241 ggagcgatcc tgcgcccgag gaggactccc cacgccacaa catcgcgcgc aagctctcgg
     3301 cggccgagga ctgggaggcc tgcgtgccgc tcctggcaga tcatttcgcc cggcacgggg
     3361 ccgggccctt cggccgccac cgggtgttcc agtggagggg gggagggctc cgggccgtca
     3421 cccttccaga ccctgtcagg ctcgaagggc tcatctccta cgagacggag cgggagccct
     3481 tggtcctgaa cacggagcgc ttcctgtcgg gtcttcccgc ccaccacgcc ctcctctacg
     3541 gtccacctgg aaccggcaaa tcctcgaccg tcaaggccgt gctcaacgag tacgcggggg
     3601 ccgggttgcg cctggtggag gtcaggaaag aagacctcgc cgacctgggc ggcgtccttg
     3661 aagacctgcg tggccgcggc ccacggttcg tcgtctacgt agacgacctc tccttcgagg
     3721 agcacgaggt cgagtacaag gccctcaagg cgctgctcga aggcagcgtc gagga
//
LOCUS       DBNGVD010000179         4943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1677228, whole genome shotgun sequence.
ACCESSION   DBNGVD010000179 DBNGVD010000000
VERSION     DBNGVD010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1677228"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..671
                     /locus_tag="AC16CM_05000"
     CDS             180..671
                     /locus_tag="AC16CM_05000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139219.1"
                     /translation="MGNEVEVRRALPDDAESLTRIAFASKRYWGYPERWISLWSSTLT
                     ITPEFVRDHEVYAAFSGGEAVGFYALTGEGHELELDHLWVSPDWIGSGMGRLLFEHAM
                     KVAASRGASIVEIEADPNAEGFYLRMGARLVGENVYDMEGERRTLPLLQVELSGKEPG
                     TES"
     gene            complement(686..1909)
                     /locus_tag="AC16CM_05005"
     CDS             complement(686..1909)
                     /locus_tag="AC16CM_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139220.1"
                     /translation="MNGRPLVLLAWSMWAVSFVLLALGVLFSYLTRATPDPNAFGLTF
                     DALLSAALLAFPTVGALVASRRHGNAIGWLFCVVGVLFGVQSAAYGWGVYALFTRPGA
                     LPVAGLAAWFSTWLFVPVLFCIPVYLFLLFPDGRLLSRHWRPVAWLAGVGLLATTLGS
                     ALSPGHLEGPPFNAIENPVGLSGGGRILDWTTIVGFWLTLGSILLAGVSLVLRYLRAG
                     GPERQQLKWFATAAALFAATSIVYSVLFARVAEQAGQISTLLAFAGIPVAVGVAILRH
                     RLYDIDVVINRTLVYGTLTAMLIAVYSVGVAATQTVFRLLTGQEQQPQLAVVISTLAI
                     AAIFNPLRRRVQTFVDRRFYRKKYDATRTLASFNSRLREETDLDSLRDDVLGVVTETM
                     QPKHTSLWLRPTRRT"
     gene            complement(2173..3282)
                     /locus_tag="AC16CM_05010"
     CDS             complement(2173..3282)
                     /locus_tag="AC16CM_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3139221.1"
                     /translation="MSAPDRVLTLRELNRATLARQMLLGRENVSPFEAVRRLVALQAQ
                     VTSPPYVGLWTRLDGFRREDLTHLMLERRVVRATLMRATLQLMTADDYLLLRPALQPA
                     LTGSMRSIAGKRLQGVDLDRLIDAARDFFEREPRPFADLRPVLADLEPDRDPSALAYA
                     VRTTLPLVQVPSGGVWGYSGKAPFTTPERWLGQPLSGSEEARDLVLRYLAAFGPAMAR
                     DVQTWSGRTRLKPVVEEMRPGLRTFRDENANELFDLPDAPLPPADTPAPPRFVPDYDN
                     LVLSHAERGRVLPEEHRKKVFLSAARVRATFLIDGFVRGTRKIERDRKTAVLQIEPFE
                     TLSGEDHDILQEEGERLVRFVAEPQGAGTFGVRFW"
     gene            complement(3279..4022)
                     /locus_tag="AC16CM_05015"
     CDS             complement(3279..4022)
                     /locus_tag="AC16CM_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139222.1"
                     /translation="MATEERDVRGVAIVTGGARGIGFEVVRQLAQQGRTVILGARDPE
                     KAGAAAKELEADGLDVRAGTVDVADDASVRGLADRVAGEFGKLDVLVNNAAAFADWSE
                     TASGADLDASRAVLDTNLFGTWRACQAFLPLIRQSDQGRIVNVASGSGSHGDPQFGLS
                     TPGGMAASYAVSKAAVLALTSKLAAELEGTGILINAVDPGLTATAPGMDEMGARPIPA
                     GAASVAWAATLPDDGPSGGFFRDGEPMPW"
     gene            complement(4104..>4943)
                     /locus_tag="AC16CM_05020"
     CDS             complement(4104..>4943)
                     /locus_tag="AC16CM_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3139223.1"
                     /translation="PTALVMLSIFSVQFGAAIAKNLFDSLGPSGTVFLRIGFAALVLL
                     VLVRPKLVGVHGGRAFLVAGLFGLALALMNLSFYQAIDRIPLGVAVTLEFVGPLGVAV
                     AGSRRVIDVLWVVLAAAGILLLAPLGAFGGMDLDPLGVAFALLAGCLWASYIFLSART
                     GSAFPGGTGLVIALCIGTVVLAPVGIAGAGSALLDPKLLLAGFGVAMLSSAIPFSLEL
                     EALRKIPARIFGVLMSLEPAMAALAGFVLLGERLGVRAVMAVLFVTIAAAGASLFGRQ
                     FGG"
BASE COUNT          919 a         1567 c         1712 g          745 t
ORIGIN      
        1 atcgagtgcg ccgaggaccg ggccgaggag atctcccggc ggacacaggc ggccatggtc
       61 agggccgggg aagacatcct ggagaaggtg cccgtcgagg tcgaagcgac gatctcgcgc
      121 gagtggagaa agtgaggttt caggcttcag gtttcaggcg tcaggcttcg gctttttctt
      181 tgggtaacga ggtcgaggtt cggcgggcgt tgcccgatga tgctgaatcg ctaacgcgta
      241 tcgccttcgc ttccaagcgg tactgggggt atccagaacg ttggatctcc ctctggagct
      301 ccacgctgac cattacaccg gagttcgtca gggaccacga ggtgtacgca gcattctctg
      361 gcggtgaggc cgtcggcttc tacgccctta cgggagaagg ccatgagttg gagctcgacc
      421 acctctgggt ctcgccggac tggatcgggt caggcatggg ccgcctgctt ttcgagcacg
      481 ccatgaaggt ggccgcctcg cggggagcga gcatcgtcga gatcgaagcc gacccgaacg
      541 ccgaaggctt ctatctcagg atgggcgcgc ggctcgtcgg cgagaacgtc tacgatatgg
      601 aaggcgagag acgcacgttg cccctgttgc aggtagagct gtcggggaag gagcctggaa
      661 ccgaaagctg acagcagatc gctcttcacg tccgcctggt ggggcgtagc cacaaggagg
      721 tatgcttggg ctgcatcgtc tccgtgacca ctcccagtac atcgtctctc aaggagtcga
      781 ggtcggtttc ctcgcgtaac ctggagttga aggaggcaag cgtcctggta gcgtcgtact
      841 tcttcctgta gaagcgtcgg tctacgaacg tttgaacgcg gcggcgcagg gggttgaaga
      901 tggcggctat cgccagcgtc gagatgacga cagcgagttg gggctgctgt tcctggccgg
      961 tgaggagacg gaacaccgtc tgggtggcgg ccacaccgac agagtagact gcgatcagca
     1021 tggcggtcag ggtaccgtag acgagggtac ggtttatgac cacgtctatg tcgtagaggc
     1081 gatggcgcaa gatggcgaca ccgacggcga cggggatgcc ggcgaaggcc aggagggtgg
     1141 agatctgacc ggcctgctcg gcgactcttg cgaaaaggac cgagtagacg atgctggtgg
     1201 ccgcgaagag cgcggcggcg gtcgcgaacc acttgagttg ctgacgctct gggccgccgg
     1261 cgcgaaggta gcgtagtacg agggagacgc ccgcgagcaa gatgctcccg agggtaagcc
     1321 agaaccccac gatcgtcgtc cagtccagta tcctaccgcc gccggagagc ccgacggggt
     1381 tctcgatcgc gttgaaaggt ggtccttcga ggtggccggg cgagagcgcg ctgcccaggg
     1441 tagtcgccag caacccaacg ccggccagcc aggccacggg cctccagtgc ctggagagca
     1501 gtcgtccgtc gggaaaaagt aggaacaggt agacgggtat acagaacaat acgggcacga
     1561 acagccaggt cgagaaccag gccgccagcc cggcaacggg cagcgccccg ggcctggtga
     1621 acagcgcgta gaccccccag ccgtacgcgg cgctctggac gccgaacagc acccccacga
     1681 cacagaataa ccagccgatc gcgttgccgt ggcgtcgcga ggccacgagc gcgcccacgg
     1741 tcgggaacgc gagcagggcg gcggagagga gcgcgtcgaa ggtcaggccg aaggcgttgg
     1801 ggtcgggcgt cgcccgggtc aagtaagaga agagcacccc gagcgccagc aggacaaacg
     1861 acaccgccca catcgaccag gcgagcaaga ctagagggcg gccgttcacg ggtcgctacc
     1921 atccggtgcc cccacgtccg ggcaagaacc tggccacaaa gcaacgcccg cggaccgtac
     1981 cttctcccgc atcgcctggg tggtgagatg ccggcgccca ctgacctgtt cctctgttct
     2041 tgccacagca ctactctaca gtagtaccgt cttcccaacg ttacgagaac cgttacgaag
     2101 cgctgcggca ccggctgata ttcgcgggcg aaaccgaagc gtgccgaaga ggcgcgttac
     2161 gtcatcgtaa cgctaccaga acctgacccc gaaggtcccg gcgccctgcg gttcggccac
     2221 gaagcggacg aggcgttcgc cctcttcttg gagaatgtcg tggtcttctc cggacagggt
     2281 ctcgaagggc tcgatctgga gtaccgccgt cttcctgtct ctctcgatct tccgggtgcc
     2341 acgcacgaag ccgtcgatga ggaaggtcgc cctgaccctg gcggccgaca gaaagacctt
     2401 cttgcggtgc tcttctggca ggacccgtcc tctctcggcg tgcgagagga cgaggttgtc
     2461 gtagtcgggc acgaagcggg gcggggccgg ggtgtccgcg ggaggaagtg gggcgtcggg
     2521 caggtcgaaa agctcgttcg cgttctcgtc gcggaaggta cgcagccccg gcctcatctc
     2581 ctccacgacg ggcttgagtc gcgtcctgcc agaccacgtc tggacgtccc ttgccatcgc
     2641 gggcccgaac gcggccaggt acctcagcac gagatcccgc gcctcctcgg aaccggagag
     2701 cggctggccg agccagcgtt ctggggtagt gaagggtgcc tttccggagt agccccacac
     2761 gccgcccgag ggtacctgca ccagcgggag cgttgttcta acggcgtagg ccagggccga
     2821 ggggtccctg tccggctcga gatcggccag cacggggcgc agatccgcga aggggcgcgg
     2881 ctcccgctcg aagaagtccc tggcggcgtc gatgaggcgg tcgaggtcta cgccctgcaa
     2941 acgctttccg gcgatggatc tcatggagcc ggtgagcgcc ggctgcaggg cggggcgtag
     3001 cagtaggtag tcgtccgccg tcatgagctg caaggtggcc cgcatgagcg tggcccgcac
     3061 gacgcgccgt tccagcatca ggtgcgttag gtcttcgcgc ctgaagccgt cgaggcgggt
     3121 ccagagcccg acatagggtg ggctcgtgac ctgggcctga agggcgacca ggcgtcgcac
     3181 ggcctcgaag ggggatacgt tctcccgccc cagcaacatc tggcgggcga gggtcgcccg
     3241 gttcaactcc cggagcgtga ggacgcggtc cggggcactc accagggcat gggctcccca
     3301 tctcggaaga agccgccgga tgggccgtcg tcggggaggg tggccgccca ggccacgcta
     3361 gcggcccccg cggggatggg tcgcgcgccc atctcatcca tgccaggcgc cgtcgcggtc
     3421 aggccggggt ccacggcatt gatcaggata cccgtgcctt cgagctctgc cgccagcttc
     3481 gaggtcaggg cgagcacggc ggccttggag acggcgtagc tagccgccat cccgcccggc
     3541 gtcgacaggc cgaactgcgg gtccccgtga gaaccgctgc cgctggcgac gttcacgatc
     3601 cgtccctggt cgctctgccg gatgaggggc aggaacgcct ggcaagcgcg ccaggtgccg
     3661 aacaggttcg tatcaagcac ggcgcgagaa gcgtcgagat cggcacccga agccgtctcc
     3721 gaccagtcgg cgaaagctgc tgcgttgttg acgagcacgt cgagcttgcc gaactccccg
     3781 gccacccggt ctgcgagccc cctgacgcta gcgtcgtcgg cgacgtctac ggtcccggcc
     3841 cgcacatcca gcccgtcggc ctccagctct ttcgcggcgg cgcctgcctt ctccgggtcg
     3901 cgcgccccca ggatcaccgt cctgccctgt tgcgcaagct gcctgacgac ctcgaatccg
     3961 atgccccggg cgccacccgt tacgatggcc acacccctga cgtctcgctc ctcggtggcc
     4021 aagatgatct cctctcgctc tccgtagaac ccagattcat gtccattcta cggatgaaga
     4081 agtctctggc taccctgtcc tgatcagccc ccgaactgtc ggccgaacag cgaggcgcca
     4141 gccgcggcga tggttacgaa cagaacggcc atcacggctc gtactccaag ccgttcgccc
     4201 agcaggacga agccggccag cgcggccatg gccggctcga ggctcatcag gacgccgaag
     4261 atgcgcgcgg ggatcttgcg cagggcctcg agttccaggg agaaggggat ggcggaggag
     4321 agcatcgcca ccccgaagcc ggcgagcaga agcttcgggt cgagcagggc ggaaccggcg
     4381 cccgctatcc ccaccggggc gaggacgacc gttcctatgc agagcgcgat gaccagcccc
     4441 gtcccgccgg ggaaggcgct acccgtacgg gcggagagaa agatatagga ggcccagagg
     4501 caaccggcaa gcaaagcgaa cgcgacgcct aaagggtcga ggtccatccc accgaacgcg
     4561 ccgaggggag cgagcaggag gatcccggcc gcggcaagca cgacccagag cacgtcaatc
     4621 acgcgccggg agcccgcgac ggccacgccg agtggaccga caaactctag cgtgacggcg
     4681 acgccgagcg ggatgcggtc gatggcctgg tagaaggaga ggttcatcag cgcgagcgca
     4741 agcccgaaga gcccggcgac gaggaaggcc ctcccgccgt gcacgcccac cagtttcggg
     4801 cgcaccagga caagtagcac gagcgcggcg aagcctatcc gcaggaagac cgtaccgctg
     4861 ggtcccaggg agtcgaagag gttcttggct atggccgccc cgaactggac ggagaagatg
     4921 gagagcatca cgagagccgt ggg
//
LOCUS       DBNGVD010000180         2283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2041907, whole genome shotgun sequence.
ACCESSION   DBNGVD010000180 DBNGVD010000000
VERSION     DBNGVD010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2041907"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16CM_05025"
     CDS             <1..265
                     /locus_tag="AC16CM_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3139224.1"
                     /translation="VEMLRMVSRRRQEEYEARRAIEKLTPREKQVLAALGEGLDSRDI
                     AEKLHITIETERTHMVNILGKLDVHSRLQALVFAARHGLVEIR"
     gene            498..815
                     /locus_tag="AC16CM_05030"
     CDS             498..815
                     /locus_tag="AC16CM_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139225.1"
                     /translation="MANEPRAYREGIAAVISQLRPTVEVEMVEPNGLDDSIRRYVPDM
                     VICSKATETLKVRVRVWVELYPENGAVSVANIGGRRVEYTEIQLPDLLSIVDKAGELS
                     QAN"
     gene            887..1207
                     /locus_tag="AC16CM_05035"
     CDS             887..1207
                     /locus_tag="AC16CM_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139226.1"
                     /translation="MGARRILVANELASYRQTLVLVIREMRPHVEVFEAASESLEREI
                     RRLRPDLVICSRVTFTIKQAVANWVELYPEFKPYSTFYIDGQHRAKSEVELSDLLALC
                     ASPP"
     gene            complement(1194..1904)
                     /locus_tag="AC16CM_05040"
     CDS             complement(1194..1904)
                     /locus_tag="AC16CM_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139227.1"
                     /translation="MAEREFRLPGSSYEELVNIIVAYGTREDAARTGDVGKLDSVHQS
                     SVSRNNAFLVEIGVLQGESRKTITRRGRELAAALARKDRTEVRNNWRAIVAASEFLQN
                     VVSAVKLREGMLYPTVQAYIAHAAGQPRNKPVMNGAGAIVEILKTAGMLREEAGELVA
                     TFGEQPQDAPQENGSPAETSEVGATVISATVDEAVVADAGTRPTVSIHVQVRCTADEI
                     EELAPRLKALVRELSTGD"
BASE COUNT          419 a          786 c          692 g          386 t
ORIGIN      
        1 cgtcgagatg ttgcgtatgg tcagccgcag gcgtcaggag gagtacgagg cgaggagggc
       61 catcgagaag ctcaccccac gcgaaaaaca ggtgctcgct gcccttggcg aggggctaga
      121 cagccgagat atcgccgaga agctgcacat caccatcgag accgagcgca cccacatggt
      181 caacatcctg ggcaagctgg acgtccactc caggctgcag gcgctggtct tcgccgcccg
      241 ccacggcctc gtcgagatca ggtagacgat cgtgaaggca tcccctatgc cggaccacgc
      301 cggtggtccc ccgacaatcc ggggatggca ccgtgctaga atcaggcccg ttcgtaccca
      361 tggccgcaca agccgacccc atgcaagaag ccgccctggg aggcataagc cctttgccga
      421 aaccgcccga taaccgaccc gagggccctg ccgacgcggg gaaccctgcc catgggcgga
      481 gcaggacgcg gatcctgatg gccaacgagc cgcgcgccta ccgcgagggg atagcggcgg
      541 tcataagcca gctacggccc acggtcgagg ttgagatggt tgaaccgaac ggcctcgacg
      601 actcgataag gcgctacgtg cctgacatgg tcatctgcag caaggctacc gagacgctca
      661 aggtcagggt gagggtctgg gtggaactct accccgaaaa cggcgccgtc tccgtggcga
      721 acatcggcgg caggcgcgtg gagtacaccg agatacaact gcccgacctc ctctccatcg
      781 tggacaaggc cggggagctc tcgcaggcca actagtccat cggggctagg aaaagatacc
      841 ccgtttttgc gatgccggta gcgtcgccga gggccaccat ctacttgtgg gcgcaagacg
      901 catactcgtg gccaacgaac tcgcctccta ccggcagacg ctcgtgctcg tgatcaggga
      961 gatgcgtccc cacgtcgagg tcttcgaggc cgcctcggaa tctctggagc gggagatcag
     1021 acgcctccgc cccgaccttg tgatctgcag ccgcgtcacc ttcacgatca agcaagcggt
     1081 cgccaactgg gtggagctct accccgagtt caagccttac tccaccttct acatcgacgg
     1141 tcagcacagg gcgaaaagtg aggtcgagct ctccgacctg ctcgcgctct gtgctagtcc
     1201 cccgtagaga gctccctgac gagcgccttg agccgcgggg ccaactcctc gatctcatcg
     1261 gccgtacacc ttacctgcac gtgtatgctg accgttggcc gcgttcccgc atccgccact
     1321 acggcctcgt ccacggtcgc ggagatcacg gtagccccca cctcggaagt ctcggccggc
     1381 gagccgttct cctgtggcgc gtcctggggt tgctccccga aggtggcgac cagctctccg
     1441 gcctcttccc gcaacatgcc ggcggtcttg aggatctcca cgatcgcgcc tgcgccgttc
     1501 atgacgggct tgttgcgcgg ctggccggcc gcgtgggcga tgtaggcctg gacggtgggg
     1561 tagagcatcc cctcgcgcag cttcacggcc gagaccacgt tctgcaagaa ctcgctggcc
     1621 gccacgatgg cgcgccagtt gttcctgacc tccgtacgat ccttgcgcgc cagcgccgcc
     1681 gccagctcgc gcccccgccg cgtgatcgtc ttcctgctct caccctgcag aaccccgatc
     1741 tccaccagaa aagcgttgtt gcggctcacg ctggactggt gtaccgagtc caacttgccc
     1801 acgtccccgg ttctcgcggc atcctcgcgg gtcccgtaag ccacgatgat attgaccagc
     1861 tcttcgtagg aagaccccgg aagccgaaac tccctctccg ccatccgaaa cctccgtggt
     1921 gagatgcctg caacaacgac ccgcattata aagcacgctc ggctccgcct cgcttgtcag
     1981 cacgctgcgc ccttcgggct ccgcttgtca gcacgctcgg gctaacgccc tcgctcgtcg
     2041 gcaacgcttc ggcttcgcct cgctttcggc cagtcgtctc tttccgtaac ggtggctcag
     2101 acaacccacc tcttccgggc atcgaatctc tagagcggca gggtgcaggc catagcggga
     2161 tctagctgac agcgagccct gtaagggcga agcgttgctg aaaagcgagc caggcgaagc
     2221 cgggcgagcg tgctgacaag ccggcgcagc ggagcgaagc cggcgtgctg acgagcggag
     2281 ccc
//
LOCUS       DBNGVD010000181         9706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1495268, whole genome shotgun sequence.
ACCESSION   DBNGVD010000181 DBNGVD010000000
VERSION     DBNGVD010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1495268"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="AC16CM_05045"
     CDS             complement(<1..1313)
                     /locus_tag="AC16CM_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139228.1"
                     /translation="MKRLLLVVTTLLLGMLLAAGMALVLGFDDEPSVAQAQVRQDDAQ
                     APGARGQAEGQGVFMRPEVGHRQAPPAREVEAPPPGLTDRGKDALREASRGEAQPAGR
                     PTVDVGAVTSAPSTNDPENNEPLPDTPSLGNSFLGVADTGSFPPDSQIAAGPNNIIAS
                     TNGAVNILDKRGNSLSSQSLANFFSPLGSEHTDVFDPWVVYDPYLDRFWMMAVSGRTT
                     ALSNIVIGLSNTQDATLGWRLWELDATVNGATDTQNWCDYPKIGFDAQAIYVTCNMFR
                     LDTTVTPNVNRYQYSKLRIMTKNQFVNNTGIFWWDFWDLREGNTGSTTLFTLQPAQMF
                     GASASNGEFLVNARGGGGTGSTLDVWRVTNAAECCNGDGVGPNLANQGHGVGSYGPSD
                     GARQPGSTTQIDNGDSRLLYAFWKNGRLSTGHTVACGGTNACGGFT"
     gene            1542..2750
                     /locus_tag="AC16CM_05050"
     CDS             1542..2750
                     /locus_tag="AC16CM_05050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3139229.1"
                     /translation="MDLNLMRELSVKTDSKMVLLILDGLGGLPLDPSGETELEAAETP
                     NLDALAARSDLGLSRPVAAGVSPGSGPGHLALFGYDPLRFQVGRGVLSALGVGFDLGE
                     KDLAARVNFATRDEEGRISDRRAGRIPSEKGEELVALLNENLKVEGVEVFVMHEKEYR
                     AVAVFRGEGLSGALADSDPQRTGLEPLTVEPTDGSQEARKSAEIANSFVEQANEILAD
                     QHPANTVLVRGFGMHPALPSFDETYDLDAAAIASYPMYKGLARLAGMELLKEGEGIPG
                     EFETLKANWDDHDFFFVHIKPTDAAGEDGDFDRKASVIEQVDAQIPGLLDLGPDALAI
                     TGDHATPAKLKSHSWHGVPFLLSSPYTLPTADAFGERPCAGGSLGVFQAEEIMGYLMG
                     HALKLNRFGA"
     gene            2772..3443
                     /locus_tag="AC16CM_05055"
     CDS             2772..3443
                     /locus_tag="AC16CM_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3139230.1"
                     /translation="MQNGGELALLEEKIVSCRLCPRLVAWREEVAREKRAAFKDEVYW
                     GRPVPGFGDPDANVVVFGLAPAAHGANRTGRYFTGDRSGDFLFSALYRTGFAGSPVSR
                     RPDDGMRLHGLWISAAVRCAPPQNKPTPAERDACLPYAARELELLDAKVVVCLGAFAW
                     DAALRLLDVRPKPKFSHGVEHWVGSLVLLGCYHPSQQNTFTGVLTPEMIDSVLSRARG
                     LTEAG"
     gene            3503..4000
                     /locus_tag="AC16CM_05060"
     CDS             3503..4000
                     /locus_tag="AC16CM_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139231.1"
                     /translation="MGFFQSDKPGTILMAILVAVALLFVGACSDGERERTGEDPPKQK
                     TEITEETTSLATMPETTGSEPPEVTLGNDSGDGPEVVIRLEGDPETTFSGLCSVGESQ
                     KVLSGEVPKRFQFDPKGQRISCRIEKRDDQDGDLKVVLAAGETTRSVQQTNTPGGVIK
                     VAYKG"
     gene            4119..4655
                     /locus_tag="AC16CM_05065"
     CDS             4119..4655
                     /locus_tag="AC16CM_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3139232.1"
                     /translation="MCRWTESDSLRKHMLAVEAAMRAYARRFGEDEEKWGVTGLLHDM
                     DYEKHPTPDEHPMVGVGELERLGYPEDVIHAIKGHADYLDVPRDTPMSKTLYAVDELS
                     GFIVACAMVRPEGLQNMKAKSVRKKMKQKSFAAAVNRDDITRGAEDLGVDLNEHIEFV
                     AGALGERSAELGFESREA"
     gene            4713..5762
                     /locus_tag="AC16CM_05070"
     CDS             4713..5762
                     /locus_tag="AC16CM_05070"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3139233.1"
                     /translation="MVRVMVVFGGRSGEHEVSLASARAIAEALGKSGRHEVVPVGITR
                     EGRWISSGEPMRELESRSGYLADGSPEVSVPPASAAEKLPAGLGSVDVVFPVLHGPYG
                     EDGTIQGMLELAGIPYVGSGVLGSAAGMDKPTMKKIFAHHGLPQVGWVAFTRKQWEED
                     REGLREEIERLGYPCFVKPANLGSSVGIRKAEGAGELGEAVDEAARHDRRIIVEEGID
                     AREIEVSVLGNEAPDVSVPGEIVIKNNDFYDYEAKYVEGGMELIAPAPIPDETTREIR
                     RIARAAFEAIDAAGMARVDFFLERGTDRLLLNEINTIPGFTPTSVYAELWATSGLPYE
                     ELLDRLVQLALERHG"
     gene            5965..6981
                     /locus_tag="AC16CM_05075"
     CDS             5965..6981
                     /locus_tag="AC16CM_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3139234.1"
                     /translation="MLFALFQEETTGIAGVVNDAGDVATDAAREPRSIFKGLGEFLQG
                     TYGYFLSGEFIGDLIASLTVVFLGILFYRILTHGLPRILRWRRREDFLDEEAVTRIKR
                     QDTAITLTRNALRYVVFAIVALVVLSIFIENPLPGVAGATILAAVIGFGAQSFLRDVI
                     AGFSIVFEGQYSVGDFVLIKPQDVSGIVEELGLRMTKIRSLSGEVSYIPNGTMQGVVN
                     YVSGQQRFNVEVQVTDAEAASRIEGALGESSELYLSPPRLVERNETDGRIRMRIVAGV
                     LPSMAWLVEDNLTAHIKAAAGEEALAAEPLTYKVDQANLRRIRSLLPQEAERNLPPQA
                     NRKL"
     gene            complement(7106..7738)
                     /locus_tag="AC16CM_05080"
     CDS             complement(7106..7738)
                     /locus_tag="AC16CM_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3139235.1"
                     /translation="MSDIPLFPLNVVLMPGAPLPLHIFEERYKQMVNECLEQEDEFGM
                     VFADESGTREVGCTARIVELVERYEDGRMLILVEGSRRFKLNNVLTGKPYYVGEVEYF
                     GEEPEEDVEALAEECIGLLERVVEAATEGSVGIEIEPPYRNLSFAIAGRVEFEPEIRQ
                     QILELTSERERLQKVRELLSAAAERLERERQTREKAQTNGHLRGDWQPGT"
     gene            complement(8014..8163)
                     /locus_tag="AC16CM_05085"
     CDS             complement(8014..8163)
                     /locus_tag="AC16CM_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139236.1"
                     /translation="MDLALLLLQAGGDGGATAVGLFRFFAGFLLVITFFCGFLGFTYW
                     ISKDE"
     gene            complement(8163..9506)
                     /locus_tag="AC16CM_05090"
     CDS             complement(8163..9506)
                     /locus_tag="AC16CM_05090"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3139237.1"
                     /translation="MPIGNLDVPIIGKNAVIGILVQTHILFAAFIIGAVLIAATSEYL
                     GMVTKQNNYERFARNLARFVVLLFASGAALAITFVLALVTLFPVFFSILQNIFFWVFL
                     VEAFMFLGQIIIVYAWYNVWDKLAYRKSLHVVFGFIAGFFGLMAMTMIDAVASFMLTP
                     AEVTSQADLVARSFLNQTMVPLNMHRFVGNFSYAGFLIAGWAGFRYLRTTREDDREYY
                     DWMGHFGLLWGFGFLIMQPVIGYGYLKGIRESSPDSFNTIMLGDKSWVFNLLVIWIAV
                     LSIASTAYFVHKLRFAVKPSETLRKLAVGALGFTALFTVLNIIPANADLVPQIGLVLG
                     QGEDTTIPLGSMYPWKYIGLIGLMLVGFFIVALYLKASANGFHWGRASRWSQIALMSC
                     AFFVVLTMVTMGYTRETARRVDNNPGYLIHDCVTLDQKLVSKHCPTEPLTEGVGE"
     gene            complement(9522..>9706)
                     /locus_tag="AC16CM_05095"
     CDS             complement(9522..>9706)
                     /locus_tag="AC16CM_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochromesubunit B of the bc complex-like
                     protein"
                     /protein_id="HIR3139238.1"
                     /translation="VLVPTIFIGLLFAVPWLDRSPQHYNAISRPFASVIGVLVVIVIV
                     LNMLMAVSRIMNFPTG"
BASE COUNT         1846 a         3092 c         3068 g         1700 t
ORIGIN      
        1 gtgaagccgc cgcaggcgtt ggtccctccg caggccaccg tgtgtccggt ggagagcctt
       61 ccgttcttcc agaaggcgta caggagcctg gagtcgccgt tgtcgatctg ggtcgtgctc
      121 cccggctgcc tggcaccatc agagggcccg tagctaccga ccccgtgccc ctggttggcc
      181 aggttgggcc ccacgccgtc cccgttgcag cactccgccg cgttggttac gcgccagaca
      241 tcaagcgtgc tgccggtccc acccccaccc ctggcgttga ccaggaactc accgttagag
      301 gcagaagccc cgaacatctg ggccggctgc agggtaaaca acgtggtcga tccagtgttg
      361 ccctccctga ggtcccagaa gtcccaccag aagatgccgg tattgttgac gaactggttc
      421 ttggtcatga tccgcagctt cgaatactgg tagcgattga cgttgggggt gacggtggtg
      481 tcgagccgga acatgttgca ggtaacgtag atggcctgcg cgtcgaagcc gatcttggga
      541 tagtcacacc agttctgggt gtcggttgcg ccgtttaccg tggcatccag ctcccacagc
      601 ctccagccca gggtggcatc ctgggtgttg gagagcccga tgacgatgtt gctcaacgcc
      661 gtggtgcggc cggagacggc catcatccag aaccggtcga gatagggatc gtatacgacc
      721 caggggtcga acacatcggt gtgctctgac cccagaggcg agaagaagtt ggccaggctc
      781 tgggaagaga gcgagttccc cctcttgtcc aggatattga cggctccgtt ggtggaggcg
      841 atgatgttgt tcggccctgc tgctatctga gagtccggcg ggaacgagcc ggtgtctgcc
      901 acgccaagga acgagttgcc cagggagggt gtgtccggca gcggttcgtt gttctcaggg
      961 tcgttcgtgg atggagcgga agttacggcc cccacatcta ccgttggcct tcccgccggc
     1021 tgcgcttccc ccctgctggc ctctctcagg gcgtccttcc ccctatcggt gaggccgggg
     1081 ggaggggcct cgacctcgcg ggctggcggc gcctgccgat ggcccacctc gggacgcatg
     1141 aatacgcctt gcccctctgc ctggccccga gcgcctggag cctgcgcgtc gtcttgacgt
     1201 acctgggcct gtgccacgga gggttcgtca tcgaacccaa gcaccagcgc catcccggcg
     1261 gcgaggagca tcccaagcaa cagcgtcgtg accacaagga gcagtcgttt catagcctct
     1321 cgccctccta acaggaaaac cctcacgggc ggtcgcattc gggccgcgat caccgcatac
     1381 ccccgcccga gccgaaagcg catcacccaa atggggtata ttcgctacta ctggaacaaa
     1441 cacaggtaat ggccgcagcc ccagaaccgg ggccgcgcct tcgagatagt catggaccac
     1501 tttgttagac ttccggcgcg ccccctacga aaggaccgac cttggatctc aacctgatgc
     1561 gcgagctatc cgtcaagacg gactcgaaga tggtcctgct catcctggac ggtctgggag
     1621 ggctcccgtt ggacccctct ggcgagacgg agctggaggc cgccgagacc ccgaacctcg
     1681 acgccctggc cgcgcgctcg gacctcgggc tctcgcgccc cgtcgcggcg ggtgtgagcc
     1741 cgggcagcgg ccccggacac ctcgcgctct tcggctacga tcccttgcgc tttcaggtcg
     1801 ggcgtggggt cctctccgcc ctcggcgtcg gcttcgacct cggggagaaa gacctggccg
     1861 cccgcgtaaa cttcgcgacc agagacgagg agggtaggat ctccgaccgc cgcgccggcc
     1921 gcatcccctc ggagaagggc gaagagctcg tcgcgctcct gaacgagaac ctgaaggtcg
     1981 aaggcgtcga ggtcttcgtg atgcacgaga aggagtacag ggccgtggcc gtcttcaggg
     2041 gcgaaggcct ctccggcgcc ctcgccgact ccgaccccca gcgtaccggg ctcgaaccgc
     2101 tcaccgtcga gccgacggac ggatctcaag aggcgcgcaa gagcgccgag atcgccaact
     2161 ccttcgtgga acaggcaaac gagatcctgg ccgaccagca ccccgcgaac acggtactgg
     2221 tgcgcggctt cggcatgcac cccgcccttc cctccttcga cgagacctac gacctcgacg
     2281 ccgcagccat cgccagctac ccgatgtaca agggcctggc ccgtctggcc ggcatggaac
     2341 tcctgaagga gggcgagggc atacccggcg agttcgagac gttgaaggcg aactgggacg
     2401 accacgactt cttcttcgtc cacatcaagc cgaccgacgc cgcaggcgag gacggagact
     2461 tcgaccggaa agcatccgta atagagcagg tagacgccca gataccgggt ctgctcgacc
     2521 tcggccccga cgcccttgct atcaccggcg accacgccac accggccaag ctaaagagtc
     2581 actcctggca tggcgtcccg ttcctcctgt cctcccccta caccctcccg accgccgacg
     2641 cattcggtga gcgtccctgc gcggggggct ccctcggcgt gttccaggcc gaagagataa
     2701 tgggctacct gatgggccac gccctgaaac tcaaccgctt cggagcgtga gagagcgccg
     2761 caaggcgtag catgcaaaac ggtggggaac tcgctttgct ggaggagaag atcgtctcct
     2821 gccggctctg tccccggctc gtcgcctggc gtgaggaggt agccagggag aagcgggcgg
     2881 ccttcaagga tgaggtttac tggggtcgtc cagtcccggg gttcggggac ccggacgcca
     2941 acgtggtggt cttcggactc gcccccgctg cccacggggc caaccggacg ggccgctact
     3001 tcacgggcga ccgctcaggc gactttctat tctcggccct gtatcggacc gggttcgccg
     3061 gcagcccggt ctcgcgcagg ccagatgatg gcatgcggct acacggactc tggatctcgg
     3121 ccgccgtccg ctgcgccccg ccgcagaaca agccgacccc ggccgagcgc gacgcctgcc
     3181 tcccctatgc ggcccgtgag ctggaactgc tcgacgcgaa ggtcgtggtc tgcctcggcg
     3241 ccttcgcctg ggacgccgcc ttgcgcctgc tcgacgtgag gccgaagccg aagttctcgc
     3301 acggtgtcga gcactgggtt ggctcgctag ttctgctcgg ttgctaccat ccctcgcaac
     3361 agaacacctt taccggcgtg ctcacccccg agatgataga cagcgtcctg tcgcgggcgc
     3421 gggggttgac ggaagcgggc tgagtggtac ccgcaaacct cagggcccca catgaccgct
     3481 tcgtcggtta gaatgcatgc ccgtgggttt cttccagtcc gataaacctg gcacgatcct
     3541 gatggccatc ctggtcgcgg tcgccctgct attcgtgggg gcctgttccg atggcgagcg
     3601 ggaacgaacg ggagaagatc cgccgaagca gaagaccgag atcacggaag agaccacctc
     3661 tctggccacc atgcccgaga cgaccggttc ggagccgccg gaggtaaccc tcggcaacga
     3721 ttccggggac gggcccgagg tggtgattcg tctggaaggc gaccccgaga ccacgttctc
     3781 cggcctatgc tcggtgggag agtcgcagaa agtactcagc ggcgaggtac ccaaacgctt
     3841 ccagttcgac ccgaaggggc aaaggatctc ctgccgtatc gagaagaggg acgaccagga
     3901 tggcgacctc aaggtcgtcc tggccgccgg cgaaaccacc cgctccgtac agcagacgaa
     3961 cacccccggc ggcgtcataa aagtcgcgta taaaggatag gtttcgagca aaggcatcag
     4021 gttagcttcc tgaagcctga aacccgtagc ctgatgccta tcgaccgaag ggagataccg
     4081 tggaacaagt agtaaaccgg gaagacgcct gggacctaat gtgccggtgg accgagagcg
     4141 actccttgcg caagcacatg ctcgccgtcg aggccgccat gcgggcgtac gcgcgccgct
     4201 tcggcgagga cgaagagaag tggggcgtga ccggcctgct gcacgacatg gattacgaga
     4261 agcatcctac gccggacgag catccgatgg tgggggtcgg ggagctggag aggcttggtt
     4321 atccagaaga cgtgatccac gccataaagg ggcacgccga ctacctggac gtaccgcgtg
     4381 acacgcccat gtcgaagacg ctctacgccg tggacgagct ctccggcttt atcgtcgcct
     4441 gcgccatggt gcgccccgaa ggcctccaga acatgaaggc caagagcgtt cgcaagaaga
     4501 tgaagcagaa gtctttcgcc gcggccgtca accgcgacga catcacgcgc ggcgccgagg
     4561 atctgggcgt ggatctgaac gagcacatag agttcgtggc cggcgcgctc ggggaacgct
     4621 ctgccgaact cggctttgaa tcccgggagg cttagaaata cggtccgcgc ctttgctacg
     4681 atgcatgggc tgaagaggag aggagcggcg gtttggtcag ggtgatggtg gtcttcggag
     4741 gccgcagcgg ggagcatgag gtgtccctgg cctcggcgcg tgccatagcg gaagcgctgg
     4801 ggaagagtgg gcgtcatgag gtcgtccccg ttggcatcac cagggagggg cgttggatct
     4861 cctccggcga gcccatgcgc gagctggaat cgaggtccgg ttacctggcc gacgggtcgc
     4921 cagaggtctc cgtccccccg gcctccgcgg ccgagaagct cccggcgggg ctaggttccg
     4981 tcgacgtcgt cttccccgtc ctgcacggcc catacggcga agacggcacc atacagggca
     5041 tgctcgagct cgccggcatc ccctacgtcg ggagtggggt gctcggcagc gccgcaggca
     5101 tggacaagcc gacaatgaag aagatcttcg cccaccacgg tctcccgcag gtcgggtggg
     5161 tcgcctttac ccgcaagcag tgggaggagg acagggaggg cctcagggaa gagatagaga
     5221 ggctcgggta tccgtgcttc gtcaagcccg ccaacctggg ctcaagcgtc ggcatccgca
     5281 aggcggaggg cgccggggag cttggcgagg ccgtcgatga ggccgcgcgc catgaccggc
     5341 gcatcatcgt cgaggagggt atcgacgcca gggagatcga ggtctccgtc ctcggcaacg
     5401 aagcccctga tgtctccgtg cccggcgaga tcgtcatcaa gaacaacgac ttctacgact
     5461 acgaggccaa gtacgtagag ggcggtatgg agctcatagc cccggccccc atccccgacg
     5521 agaccacacg cgagataagg cgcatagccc gcgcggcctt cgaggccata gatgcagcgg
     5581 gaatggcgcg cgtcgacttc ttcctggagc gcggcacgga ccggttgctg ctcaacgaga
     5641 tcaacactat ccccggcttc acccccacaa gcgtctacgc ggagctctgg gccaccagcg
     5701 gcctccctta cgaggagcta ctagatcggc tcgtgcaatt ggccctggaa cggcacggtt
     5761 gaggtaggtt ttggcatcag gctgcaggca tcgggctaca gggcttgcag cggatggcac
     5821 gcgatttgac cttctctggt gggatccgtc ggctttcgcc gggcgcgatt tgtcatcgaa
     5881 cttgacgcct gtggcctgca gcctgtagcc tttgacccct ccgctcggct tgcagccgca
     5941 cgcagagaat actttaatgg acgcatgctt ttcgccttgt ttcaggaaga gacgaccggg
     6001 atcgcgggcg tggtcaacga cgctggagac gtggccacgg acgctgcgcg ggaaccccgc
     6061 tccatcttca aggggctcgg ggagttcctc cagggcacct acggctactt cctgagcggc
     6121 gagttcatag gagacctcat cgcttcgttg actgtggtgt ttcttgggat cctgttctac
     6181 cggattctga cgcacgggtt gccccgcatc ctgcggtggc gccggcggga agacttcctc
     6241 gatgaggagg ccgtgacccg catcaagcgc caggacacgg ccatcaccct tacccgcaac
     6301 gccctgcgct acgtggtctt cgcgatcgtc gcgctggtcg tgttgagcat cttcatagag
     6361 aacccgctcc ccggcgtggc gggtgccacg atcctcgccg cggttatcgg tttcggggct
     6421 caaagctttt tgcgggacgt gatagccggt ttctccatcg tcttcgaagg gcagtacagc
     6481 gtcggggatt tcgtcctgat aaagccacaa gacgtatctg ggatcgtgga agagttgggc
     6541 ttgaggatga ccaagatccg gtctctctcc ggcgaagtta gctacatccc gaacggcacg
     6601 atgcagggtg tggtcaacta cgtctcgggc cagcaacgct tcaacgtgga ggtccaggtc
     6661 accgacgccg aggcagcctc caggatcgag ggggcgctag gcgaatcgtc cgagctctac
     6721 ctgagcccgc cccgcctcgt ggagcgcaac gagaccgacg ggcgcatcag gatgcgcata
     6781 gtggccggcg tccttccctc catggcctgg ctcgtagaag acaacctcac ggcgcacata
     6841 aaggctgccg ccggcgaaga agccctcgcc gcagaaccgt tgacctacaa ggtagaccag
     6901 gctaacttga gaaggatcag gtctctgctc ccccaggaag cggaacgcaa cctgccgccg
     6961 caggcgaaca ggaagctcta gacgctttca gccatcagct ttcagctatt agccgtcagc
     7021 aatatgtcgc ctgccatctt cgtagggagt tcgcgacgaa ccttctccac aaaatcactg
     7081 gctgaaagct agaagctgat agctcctaag tccctggctg ccagtcgccg cgcaggtggc
     7141 cgttggtctg agctttctcg cgggtctgcc gttcacgctc cagcctctcc gccgccgcgc
     7201 tcaggagctc tctgaccttc tgaagccgtt ctcgttccga ggtgagctcc aggatctgct
     7261 gcctgatctc gggctcgaac tctacgcgtc cggcgatcgc gaacgagagg tttctgtagg
     7321 gcggctctat ctctatgcca acggaaccct cggtggctgc ctccacgacg cgttcgagca
     7381 gcccgatgca ctcctcggcc agcgcctcca cgtcctcctc gggctcctcc ccgaagtatt
     7441 cgacctcgcc cacgtagtag ggcttgcccg tgaggacgtt gttcagcttg aaacgcctcg
     7501 aaccctctac caggatcaac atccgcccgt cctcgtaccg ctcgacgagc tccacgatcc
     7561 tggccgtgca acccacctcg cgcgtcccgg actcatcggc gaagaccatc ccgaactcgt
     7621 cctcctgctc gaggcactcg ttcaccatct gcttgtaacg ttcctcgaag atgtgaagcg
     7681 gcaatggggc acccggcatc agcaccacgt tcagcgggaa aaggggtatg tccgacacac
     7741 gagctccttc cgctctttga ggccattcta acagcacgcc ggcttcgctc cgctggcttg
     7801 tcggcacgct ccctacggtc gcttgtcagc aacgcttcgc cgacttcgcc aggctctcct
     7861 tcggccagat cccgttgcgg cctgcacccc cccgctgtga agattcgata cccaggaaag
     7921 ccttatgtcg aagctgccgt tacggagaga gacgaccagc cgaaagcgag agccttcagg
     7981 ctcgaagcgt gctgaacggc gcctaggcgc cgttcactcg tctttgctga tccagtaggt
     8041 aaagcccagg aagccgcaga aaaacgttat gaccaggaga aagccggcga agaacctgaa
     8101 gaggcccacg gcggtcgcgc ccccatcgcc tcccgcctgc aggagtagca gggcaagatc
     8161 cattactctc ccaccccttc ggtaaggggc tcggtcgggc agtgcttgct gacgagcttc
     8221 tggtcaagcg tcacgcagtc gtggatcagg tatccggggt tgttgtccac ccggcgtgcc
     8281 gtctcacgcg tgtagcccat ggtcaccatg gtaagcacca cgaagaacgc gcacgacatc
     8341 agcgcgatct ggctccaccg gctggcccgt ccccagtgga agccattcgc cgaggccttc
     8401 aggtaaaggg cgacgatgaa gaagcctacg agcatgagcc cgatcaggcc gatgtacttc
     8461 cacgggtaca tgctgccgag ggggatggtg gtgtcctcgc cctgccccag aacgagcccg
     8521 atctgtggca cgaggtcggc gttggccggg attatgttga gcaccgtgaa cagagcggtg
     8581 aagcccaggg cgcccacggc tagcttgcgt aacgtctccg aaggcttgac ggcgaaccgc
     8641 agcttgtgca cgaagtaggc cgtcgaggcg atgctaagga ccgctatcca gatcaccagc
     8701 aagttgaaga cccaggactt gtcgccgagc atgatggtgt tgaaggagtc gggggagctc
     8761 tcgcggatac ccttcaggta accgtagccg atgaccggct gcatgatcag gaagccgaag
     8821 ccccacagga ggccgaagtg ccccatccag tcgtagtact ccctgtcgtc ctcccgcgtg
     8881 gtcctcaggt acctgaaccc ggcccagccg gcgatcagga accctgcgta ggaaaagtta
     8941 ccgacaaagc ggtgcatgtt gagcggcacc atggtctggt tcaggaagct tctcgccacg
     9001 aggtcggcct gcgaggtcac ctcggcgggc gtgagcataa acgaggcaac ggcgtcgatc
     9061 atggtcatcg ccatcaggcc gaagaagccg gctatgaagc cgaagaccac gtgcaggctc
     9121 ttgcggtagg cgagcttgtc ccacacgttg taccaggcgt agacgatgat gatctggccc
     9181 aggaacatga acgcctcgac caggaagacc cagaagaaga tgttttgcag gatggagaag
     9241 aagaccggga acaacgtcac cagggccagc acgaaggtga tggccagggc ggcgccggag
     9301 gcgaagagca ggaccacgaa gcgggccagg ttcctggcga agcgctcgta gttgttctgc
     9361 ttggtcacca tcccgagata ctcgctcgtg gccgctatca ggacggcccc gatgatgaac
     9421 gcggcgaaca ggatgtgagt ctgaacaaga atgccgatta cggcgttctt gccaattatc
     9481 ggtacgtcga ggttgccaat aggcaccgct agatcccctc tctagcccgt cgggaagttc
     9541 atgatcctgc tgaccgccat gagcatgttc aggacgataa cgataaccac cagaacgccg
     9601 ataaccgaag cgaacggcct gctgatcgcg ttgtagtgct gcgggctgcg gtcaagccac
     9661 gggacggcga agaggagccc gataaagatc gtgggcacca agacgg
//
LOCUS       DBNGVD010000182         2863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_949618, whole genome shotgun sequence.
ACCESSION   DBNGVD010000182 DBNGVD010000000
VERSION     DBNGVD010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_949618"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1627
                     /gene="lysS"
                     /locus_tag="AC16CM_05100"
     CDS             146..1627
                     /gene="lysS"
                     /locus_tag="AC16CM_05100"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3139239.1"
                     /translation="MNQTGSSTGISIPAWAERVADTLGPGPHVVVSGISVSGNIHAGN
                     LREVLVAEAVANALRARGEEVRFVFHADTIDPLRKIAPGIPKSYEEYIGHSLSRVPDP
                     EGCHVSYSEHFLAPFEEALREMGMDVEVIRSHELYERGVYTDVTSESIEHTAALRGIL
                     QEVTNRTMPEHWSPYLPRGSSGKLTGMRVLEHLPEQTSVVFADEDGFEEAADYSRGEG
                     KLGWRVELAARWKALGVTFEPFGKDHTSRGGSTDTADRMANEVFHYPVPGRFEYEWIG
                     LKGRGDMSSSRGIVLLPLDLLRIMPPGAVRRIILGRDPARRFDIDLGAGFPRFMDEYR
                     SEAGEPFVPFNHLVTVAQTVGDDVDTAAEMLRRGGYGEAAENGEKLARDLAYARNWAA
                     EWAPESMRLGLLDEEASKEAAAGLDDEQRAYLREVSERLVPEMEGEAVQDVLYSTAIG
                     RGLKPKKAFAAVYTVLLGKTSGPKAGPFIAGLTPEQVSQRLSV"
     gene            1947..>2863
                     /locus_tag="AC16CM_05105"
     CDS             1947..>2863
                     /locus_tag="AC16CM_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3139240.1"
                     /translation="MFERFTERARKVVVLAQDEARHFNHNYIGTEHLLLGLLREDEGV
                     AAQALYSLNVNLDEVREQVESIVGYGEEGTGAQAPFTPRSKKVLELALREALQLGHNY
                     IGTEHILLGLVRESEGVAARVLSNLDVDPDKVRREVVRRLGGGRSRSRGGGGTEAGSG
                     RGVEAKRPKTRQLDQYGRNLTAYAEEDKLDPVIGRNQEIARIMQILVRRTKNNPVIIG
                     EPGVGKTAIVEGLAEEIAEERVPDILAGKEVYTLDLGALVAGSKYRGEFEERLKKILK
                     EITDHGDIILFIDEIHNLVGAGAAEGAIDA"
BASE COUNT          555 a          875 c          975 g          458 t
ORIGIN      
        1 ccgtgttgaa gaagaaggtg ggggagaagg tcatggtctc caccccccgc ggcgcgacgg
       61 agtacgagat cgtgaacgtc gaggcggcca gctagtccct ccgaacgacc cctcgttcga
      121 ccaccgaatc ccatgggagc atcgcatgaa ccagaccggt agctcgaccg gcatttccat
      181 accagcctgg gccgagaggg tcgccgatac gcttgggccg gggccgcacg tcgtggttag
      241 cggcatcagc gtctctggca acatccacgc tggcaacctg cgcgaggtcc tcgttgccga
      301 ggcggtggcg aacgccctga gggcgcgcgg tgaggaggtc aggttcgtct tccacgccga
      361 caccatagac ccgctgcgca agatcgctcc cggcatcccg aagagctacg aggagtacat
      421 cgggcacagc ttgtcgcgcg tgccggatcc cgagggctgc catgtctcct actccgagca
      481 cttccttgcg cctttcgaag aggcgctgcg ggagatgggg atggacgtcg aggtcatccg
      541 ctcgcacgag ctttacgagc gtggcgtcta caccgatgtg accagcgagt ccatcgagca
      601 cacggccgcg ttgcgcggga tcctgcagga agtgacgaac cggacgatgc cggaacactg
      661 gtcgccctac ctgccacgag gctcctccgg aaagctgacc gggatgcgcg tcctggagca
      721 cctgcccgag cagacaagcg tcgttttcgc cgacgaagac ggcttcgaag aggctgcaga
      781 ctattccagg ggcgaaggca agctgggctg gcgggtcgag ctcgcggccc gctggaaagc
      841 cctgggcgtc accttcgagc ctttcggcaa agatcacacc agcaggggag gctcgacgga
      901 caccgcagac cggatggcca acgaggtctt tcattatccg gtgcctggac gcttcgagta
      961 cgagtggatc gggctcaaag gacgaggaga tatgagcagt tccaggggca tcgtgctctt
     1021 gcccctggat ctcctgcgga taatgccccc tggcgccgtg cggcgcatca ttctgggccg
     1081 agatcccgcc cgccgcttcg atatcgacct cggggccggc tttccgcgct ttatggacga
     1141 gtatcgctct gaggccggcg aacccttcgt tcccttcaac cacctggtca ccgtcgccca
     1201 gaccgtcggt gacgacgtgg ataccgcggc ggagatgctg cgccgcggcg gttacgggga
     1261 ggcggcggag aacggggaga agctggcgcg tgacctcgcc tacgcccgga actgggcagc
     1321 tgagtgggcg ccggagtcca tgcggctcgg gttgctggac gaggaagcgt cgaaggaggc
     1381 cgcggcagga ctcgacgacg agcagcgcgc ctatctgcgc gaggtctcgg agaggctcgt
     1441 gcccgagatg gaaggcgagg ccgtgcagga cgtgctctac tcgacagcca tcgggcgggg
     1501 gctcaaaccg aagaaggcct tcgcggccgt ctatacggtc ttgctcggca agacgagcgg
     1561 gcccaaggcc ggtcctttta tagccgggtt gacgcccgag caggtttctc agcggctctc
     1621 agtgtaagcc ctgacacctc tgcgcgggcg ttcaggaggt atcttggagg tggcgctata
     1681 gaggggcgac ggtccccgcc gcacacgagg cgtagcgctt atagcgtaaa ggttacgaag
     1741 gacgccgaaa tgagagtgta acggttgtat actccgtgta gccggcaacg cgttagagag
     1801 ggacgtacat cagagaggcg cagttttttc gaaaggcaga ttttagcgat gtgtaatccg
     1861 tcgatgttta acgatcgtat acaggtacat attgtcgtga cccccgaagc cgtgaaggac
     1921 cctcgaagcg gaggaggatc ccgctaatgt tcgaacgttt cacggagcgg gcccgcaagg
     1981 tcgtggtgct cgcgcaggac gaggcccgtc atttcaacca caactacata ggcacggagc
     2041 acctgctcct cggtctcctg cgtgaggacg agggcgttgc cgcgcaggct ctctactccc
     2101 tcaacgtgaa cctcgacgag gtgcgcgagc aggtcgagag catcgtcggt tacggcgagg
     2161 aaggcaccgg ggcgcaggcc ccgttcacgc cccgctccaa aaaggtgctc gagcttgccc
     2221 tcagggaagc cctgcaactg gggcacaact acatcggcac cgagcacatc ctgctcgggc
     2281 tcgtaaggga gagcgagggc gtcgccgccc gcgtgctctc caatctcgac gtcgacccgg
     2341 acaaggtcag gcgtgaggtc gtcaggaggc tcgggggcgg ccgtagccgc agccggggcg
     2401 gcggtggcac cgaggccggc agcgggcgcg gggtcgaggc caagcgtcca aagacccgcc
     2461 agctcgacca gtacggcagg aacctcacgg cctacgccga agaggacaag ctcgaccccg
     2521 tcatcggcag gaaccaggag attgcgagga tcatgcagat cctcgtccgg cgtaccaaga
     2581 acaacccggt gatcatcggc gagcccggcg tcggcaagac cgccatcgtc gaggggctgg
     2641 cggaggagat cgccgaggag cgcgtcccgg acatactggc cggtaaagag gtctacacgc
     2701 tggacctcgg ggcgctcgtc gcgggttcca agtaccgcgg cgagttcgag gagcgcctca
     2761 agaagatcct gaaggagatc acggaccacg gcgacatcat cctgttcata gacgagatcc
     2821 acaacctcgt cggggcgggt gccgccgagg gcgccatcga cgc
//
LOCUS       DBNGVD010000183         2866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4958255, whole genome shotgun sequence.
ACCESSION   DBNGVD010000183 DBNGVD010000000
VERSION     DBNGVD010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4958255"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16CM_05110"
     CDS             <1..468
                     /locus_tag="AC16CM_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3139241.1"
                     /translation="LILVVFFGSLRIPLVILLAVPLTTVGAFGALFLTNTAISVPSLL
                     GLLLLIGIVVSNAILLVDFATRFRDQYETADEAILEAGRARLRPILMTAFATIFALMP
                     LALGLSGGGNGLISSSLAITVVGGLATSTFLTLLVVPVGYSLARRNQKRGKKV"
     gene            474..965
                     /locus_tag="AC16CM_05115"
     CDS             474..965
                     /locus_tag="AC16CM_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139242.1"
                     /translation="MVRGLETGERAPYFSLLAAGSGRRVDTQNVVGRRVVLVFHLQGT
                     APTAREINRAVRLRYPDPEDVLIASVIDLSIVPPFYWMSVGLILGQAYDRAAQELPPG
                     ADPAEYVVILPDWGGLVSRRYDVGNTGRAAAIVVLDEDGSVAASYQGERPVESVLEVI
                     GRT"
     gene            1091..1297
                     /locus_tag="AC16CM_05120"
     CDS             1091..1297
                     /locus_tag="AC16CM_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139243.1"
                     /translation="MREVLRDRFEDIPVQVPRSEIEALGVEIGLEDPLEAARVFDRLK
                     GVSWRGDYVRTEEGWIAAWVEEVN"
     gene            complement(1341..>2866)
                     /locus_tag="AC16CM_05125"
     CDS             complement(1341..>2866)
                     /locus_tag="AC16CM_05125"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3139244.1"
                     /translation="SATGPAGTKPAFWVDGNTHAGEVTGSMAALYLIEHLLENHGSED
                     LPTRLLDEQAFYILPRLSPDGAERYLTTPHTLRATPRPWPEPEEEPGLQPGDVDGDGN
                     IVQMRVADENGEWRVSDGDERLLVPRAPDEADPNATYYRLYREGTFKDFDGFERKIAR
                     PLYGLDMNRQYPYHWQDEERDAGPYPLSEPESRAQVDFVLDHDNIFGVHTYHTYCGAI
                     LRPYSNRPDEEMREDDLAIYKALGDRGTEITGYPNISVYHDFAYDQKKYITGAFDDWA
                     YDYYGVFAFTIEFWSMAEAAGVKVEDFIEFARNPPEEDALKMLAWNDRELEGEGFVPW
                     RPFDHPQLGRVEVGGWKTKFTFQNPPPRYLKAECEKLARFALAHASSAPRLEARLETE
                     RLAPGLRRVELVVENKGYLPTNITSVAVDKKLAKPVKATLGLPSGAGLASGQLEEDLG
                     HLAGRSALAASRWQNPPFFQGLPSDYASRKVWVVRGDGPIEIEVRGGRAGTVHLETG"
BASE COUNT          440 a          902 c          978 g          546 t
ORIGIN      
        1 ctgatcctgg tcgtcttctt cggctcgctg cgcatccccc tcgtgatact gctcgccgtg
       61 cccctgacca cggtcggtgc cttcggcgcg ctgttcctga ccaacacggc catctcggtg
      121 ccgtcgctgc tcggcctcct gctactcatc ggcatcgtgg tctcgaacgc catcctgctc
      181 gtggacttcg ccaccaggtt cagggaccag tacgagacgg ccgacgaggc catcctggag
      241 gccggccggg cgcggctgcg tcccatcctc atgacggcct tcgccaccat cttcgccctc
      301 atgccgctgg cactcgggct ctcgggcggt ggtaacggcc tgatctcctc cagcctggcc
      361 ataacggtgg tgggggggct cgccacctcc accttcctga ccctgctggt cgtgcccgtc
      421 ggctactcgc tggccaggag gaaccagaaa cgcggaaaga aggtctagag gctttggtcc
      481 ggggacttga gacaggtgag agggcgccgt acttcagcct gctggccgct gggtcggggc
      541 ggcgggtgga cacgcagaac gtggtggggc gccgggtcgt gctcgtcttc cacctgcagg
      601 gcacggcccc gacggcgcgt gagatcaacc gcgccgtccg cctccgttac cccgaccccg
      661 aggatgtcct catcgccagc gtcatagacc tctccatcgt gccgccgttc tactggatgt
      721 ccgtcggcct gatcctgggt caggcttacg acagggccgc acaggagctg ccccccggcg
      781 ctgatcctgc cgagtacgtc gtcatcctcc ccgactgggg cggcctcgtc agccgccgct
      841 acgacgtcgg gaacacgggc cgggcggcgg ccatcgtcgt gctggacgag gacggtagcg
      901 tcgcggcctc ctaccagggg gaacggccgg tcgagagcgt actggaggtg atcgggcgga
      961 cctagacccc gcagagtgtt tttgacgaca gtctcgctcg agggggagag gcagactgtc
     1021 tccataggag aggaggtcgg ccatcagaga gggaagatac cgtacccccg atagggagca
     1081 gcagatcagg atgcgcgagg tcctgaggga caggttcgaa gacatccccg tccaggtccc
     1141 gagaagcgag atagaagccc tcggcgtcga gataggcctc gaagacccgc tcgaggcggc
     1201 cagggtcttc gaccgcctga agggagtctc ctggaggggc gattacgtca ggaccgaaga
     1261 gggttggatc gccgcctggg tcgaagaggt gaattagagc ggtcagccac cagcttccga
     1321 ccttgcttgt tgctgatggc ctaacccgtt tcgaggtgta ccgtgcctgc ccttccgccg
     1381 cggacctcga tctcgatggg accgtcgccg cgtacgaccc agaccttccg gctggcgtag
     1441 tcggagggca ggccctgaaa gaagggtggg ttctgccacc tgctggcggc gagcgcggag
     1501 cgccccgcga ggtggccgag gtcctcttct aactgtccgg aggccaggcc cgcacctgac
     1561 ggtagcccga gggtcgcctt caccggtttg gccagcttct tgtctaccgc cacgctggtt
     1621 atgtttgtcg gcaggtagcc cttgttctcg acgacgagct cgacgcgacg gagacccggc
     1681 gcgagccttt cggtctccag gcgggcctcc aggcgggggg ctgaagaggc gtgggcgagg
     1741 gcgaagcggg ccagtttctc gcattcggcc ttcaggtacc tgggcggcgg gttctggaac
     1801 gtgaacttgg tcttccagcc gccaacttcg acgcggccca gctgggggtg gtcgaagggt
     1861 ctccagggta cgaagccctc gccttctagc tccctgtcgt tccaagccag catcttcagg
     1921 gcgtcctctt ccggtggatt acgggcgaac tcgataaagt cctccacttt cacgcccgcg
     1981 gcctcagcca tggaccagaa ctcgatggta aaggcgaaga cgccgtagta gtcgtaggcc
     2041 cagtcgtcga aggcgccggt tatgtacttc ttctggtcgt aggcgaagtc gtggtagacg
     2101 gagatattgg ggtagccggt gatctccgta cccctgtcgc ccagcgcctt gtagatcgcg
     2161 aggtcgtcct ccctcatctc ctcgtccggc ctgttcgagt agggacgaag tatcgcgccg
     2221 cagtaggtgt ggtaggtgtg cacgccgaag atgttgtcgt gatctagcac gaaatcgacc
     2281 tgcgcccttg attcgggttc ggagaggggg tagggtcccg cgtccctctc ctcgtcctgc
     2341 cagtggtacg ggtactggcg gttcatatcc agcccgtaga ggggacgcgc gatcttgcgc
     2401 tcgaaaccgt cgaagtcctt gaaggttcct tctctgtaga gccggtagta ggtggcattc
     2461 ggatcggcct cgtcgggcgc gcgggggacc agaagcctct cgtcgccatc cgagacgcgc
     2521 cattcgccgt tctcgtcggc cacgcgcatc tgcacgatat tgccgtcccc gtccacgtca
     2581 ccgggctgga gaccgggctc ctcttccggc tcgggccagg ggcgcggggt ggcccttagc
     2641 gtatgggggg tggtgaggta gcgttcggcg ccgtcggggg agaggcgggg taggatgtag
     2701 aaggcctgct cgtcgagcag gcgggtcggt aggtcctccg agccgtggtt ctcgaggagg
     2761 tgttcgatca ggtagagcgc ggccatcgag ccggtaacct caccggcgtg ggtgttgccg
     2821 tccacccaga aggctggttt cgtgccagcc ggacccgtcg ccgagt
//
LOCUS       DBNGVD010000184         7602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4410451, whole genome shotgun sequence.
ACCESSION   DBNGVD010000184 DBNGVD010000000
VERSION     DBNGVD010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4410451"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1213)
                     /gene="fmt"
                     /locus_tag="AC16CM_05130"
     CDS             complement(296..1213)
                     /gene="fmt"
                     /locus_tag="AC16CM_05130"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3139245.1"
                     /translation="MKVAFAGTPPFAATILRALISSSHEVGIVISQPDRRSGRGRRAT
                     QTPVAELALQHDLPLEQPARVSELDEALSSLDALVVAAYGQILLPSTLYATRYHAYNV
                     HASLLPRYRGAAPVERAIMNGESETGVTIMKMDEGLDTGPMALQVPVRIPPEMNAGEL
                     TDLLANTGAKAIVEALNLIESNELTLVEQDNLDATYASKPGGEERIIGWHHGATEVHD
                     LVRALSPRVGARTHHDRVDGPVKIWRSRVAGTGTPPSHPGHIRAESGRMVVSCGSGSL
                     EVLELQAPGGRRLPTREFLLGRPLEGTFR"
     gene            complement(1222..1719)
                     /gene="def"
                     /locus_tag="AC16CM_05135"
     CDS             complement(1222..1719)
                     /gene="def"
                     /locus_tag="AC16CM_05135"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3139246.1"
                     /translation="MTLDIRKFGDPVLKTRAAPVETFDDSLASFTEEMLATMRENDGV
                     GLAANQVGRLKRVLVASVEDEDYVVVNPVLSEVSETKEPGQEGCLSIPGINVEVERAT
                     AVTVTGQDASGEPVRLEARDMLARVFQHEVDHLDGVLILDRTDRQSRKDAMREWREQL
                     LAQQG"
     gene            complement(1724..3919)
                     /locus_tag="AC16CM_05140"
     CDS             complement(1724..3919)
                     /locus_tag="AC16CM_05140"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR3139247.1"
                     /translation="MRVSLLIPTHALPPLSYRIPKRYRAEIGLGSAVVAPLSGRQRLG
                     IVVGFEGEEDRAREDVLSVVPDLSPGPDLLRLCLAICDTAAVPLPTVLRAALPPGIDA
                     GRYLVLRPIQDQPWEPGSTVTRTTLKRVLGPDGLRREEASGRIVLSPSALQPRAVEWA
                     VVRAAADPDLSRAPRQRDLFVALKDVGGASPTSVLLGKTGASRTALRELARRGALRLV
                     RRPEPAPLVVAEGTADAPARPFSRPAAGAVRNGGAFLWRITSREEPDAVAAVVAATLR
                     GGGQALVLVPEVRAIGPLVEHLRRALPAGRTIAEYHGGLGRARAAVYGAARDGNVDVV
                     VGTRTAALLSLARPGSTCVVDEPNDAHMAGPGHEGLPVHVRDVALERSRVDGAAVFFL
                     SPSPSLRLYAREVRSREGLRELAASRPERWPAVRIVDMRDSGATLSSTLIEACRRCVE
                     GGGRAGLVLRRLGYAPTVSCSRCGTMKLCPNCELPLLLHGRNGPLVCTRCGHREKLGE
                     CSNCGSERLRPTGLGVERVRDQLSEALGTQIGLITADEREYADAPVVVGTAPRLLDGG
                     WDAVILPDADAFLGASNVGAVERAFRLFFRAGEAARGLLIVQTRLPEHYALRAGVRGD
                     YEAFAAAELPRLRTLAYPPFGHFASLTFDGSESAVLGAVESRLRPTLGPEVEMSSPVP
                     LGRTGGRSAWRVLLRSRRRPAVARAAADAARAAARTHGLTVRVELDQEV"
     gene            complement(3974..5212)
                     /gene="metK"
                     /locus_tag="AC16CM_05145"
     CDS             complement(3974..5212)
                     /gene="metK"
                     /locus_tag="AC16CM_05145"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3139248.1"
                     /translation="MVERAAEPTTWNAADDQKLRTSHLFTSESVTEGHPDKIADQISD
                     AILDAALADDPMSRVACETLVTTGLVVVAGEITTSTYVDIPTLVREKIAEIGYTRAKF
                     GFDAATCGVIVSIDPQSDDIAVGVDRALEIRNDDETLDEIGAGDQGMMFGYACEETPE
                     LMPLPITLAHALTRRLSEVRKSHALDYLRPDGKSQVTVRYEGDRPVAVEKVLISAQHR
                     AGAEATIRDDLMETVILPVMPRDLFDPATAEVLVNPTGRFVTGGPQGDTGLTGRKIIV
                     DTYGGSAPHGGGAFSGKDATKVDRSASYAARWVAKNLVAAGLAGRCLVQVAYAIGVAR
                     PVSVMVETFGTARVDPATLGALVQEHFDLRPGAIIRDLGLRGPIFARTAAYGHFGRRE
                     PGFSWEETARADDLRRAAGL"
     gene            complement(5215..6285)
                     /gene="coaBC"
                     /locus_tag="AC16CM_05150"
     CDS             complement(5215..6285)
                     /gene="coaBC"
                     /locus_tag="AC16CM_05150"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3139249.1"
                     /translation="MILISVGAAPGALAIPAVVRKLAEAGQQVEVILGPRARHFVGPA
                     AFGDAALVDEPSETPEATLYAPATSETVARLARGLDRGAGALGVIAPDLDAGTAEHPA
                     VRENLDLLRADGHRIVEGSGAGMADPGSIVAGLLGGLDGPMQGMRLLVTAGGTREPID
                     SVRFVGNRSSGKMGLAVAREAVRRGADVTVVSANVEMVEPGTETRAVESAGELRDAVV
                     ELAARADALVMAAAVSDFTPASPVREKIRRGSETMSLELVPTDDILASVSGRYPGLFV
                     VGFAATHGDALEDARDKLGRKGADLVVGNDISGEGVGFGADENEVYVVGREEEHFVPR
                     ASKSAVAGIILDTMMNEMERQR"
     gene            complement(6309..6671)
                     /gene="rpoZ"
                     /locus_tag="AC16CM_05155"
     CDS             complement(6309..6671)
                     /gene="rpoZ"
                     /locus_tag="AC16CM_05155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3139250.1"
                     /translation="MLNDLKVDELLGKVDSRYGLAVASAKRARQINEYTSTLGLNDAL
                     GIPGPQVHTRSQHPLTIAIEELRQDKLNVSFRQPLEELDEEAGAAGGDDFVSPPSGGG
                     VPQASDVDTGAPQDEGVA"
     gene            complement(6664..7182)
                     /gene="gmk"
                     /locus_tag="AC16CM_05160"
     CDS             complement(6664..7182)
                     /gene="gmk"
                     /locus_tag="AC16CM_05160"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3139251.1"
                     /translation="MIRASLEAVPELAYSVSATTRNPRPGEENGRDYVFLSRGEFERW
                     IGEGRFLEWAEYSGNLYGTPEQKVEELLGEGKSVILEIELQGARQVKTKRPDAVMVFV
                     RAPSLEETRRRLTGRATESEEAMESRLATAIGEVAATDEFDHEIVNADRTQARNDMIE
                     TMESIVRGGNGA"
     gene            complement(7221..7544)
                     /gene="mihF"
                     /locus_tag="AC16CM_05165"
     CDS             complement(7221..7544)
                     /gene="mihF"
                     /locus_tag="AC16CM_05165"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIR3139252.1"
                     /translation="MLRRAPERTVDERRNALEEANRVRFARAGTKRDLKSGSLAIYDL
                     LMDPSEELKGAKVEEMLLAVKGMGRIKVTRVMRDAGISRSKTLVGLTHGQRDRLIRAL
                     GCEEG"
BASE COUNT         1198 a         2638 c         2470 g         1296 t
ORIGIN      
        1 agccggtccc caatctcgct cgccacaagc acgtcgagcg ctaccctgcc gtaaccccca
       61 gcccccacta tcacgaccct catgtcccgg tcgatgctta ctgtctgtac ctgaacctcg
      121 ggttcaggta cgttcgtagc tatttccggc tctcttatct ccctgtcctc ccaacgggaa
      181 tcacgcccga cgaccagggc agtttagcac cgttgctggc atgttgctct cttacgggca
      241 tcgtctacag ggcaccttcg atcgcgccgt gcgtggggag aaaaccggcc tccggtcagc
      301 ggaacgtgcc ctcaagaggg cggccgagaa ggaactcgcg ggtggggagg cgccgaccac
      361 cgggcgcctg tagctccagc acctcgaggg accccgatcc gcaggacacg accattcgcc
      421 cgctctcggc ccggatgtgg cccggatggg aaggcggcgt tcccgtccca gccacccggg
      481 agcgccagat cttgaccggc ccgtcgaccc ggtcgtgatg ggtcctcgca cccaccctcg
      541 gtgagagggc tcttaccagg tcgtgtacct ccgtggcacc atgatgccag ccgatgatcc
      601 gctcctcgcc ccccggcttt gaagcatagg tggcatcaag attatcctgt tcgacgaggg
      661 ttagctcatt ggactcgatc aggttcaaag cctctactat agctttagct ccagtgttgg
      721 cgagaagatc cgtgagctcg ccggcgttca tctctggcgg tatgcggacg ggaacctgga
      781 gggccatcgg gccggtatcc aggccctcgt ccatcttcat gatggtcacg ccggtctcgg
      841 actcaccgtt catgatggcg cgctcgaccg gggccgcgcc gcggtatctt ggcaggagtg
      901 aggcgtggac gttgtaggca tggtatctgg tggcgtagag ggtgctgggg agcaggatct
      961 ggccgtaggc ggcgaccacc agggcatcga gagaggagag agcctcgtcc agctcggaga
     1021 cgcgggctgg ctgctcgagg ggcaggtcgt gctggagggc tagctcggcg accggtgttt
     1081 gggttgctct gcgtccccgc ccgctgcggc ggtcgggctg cgagatcaca atccccacct
     1141 catgggaaga ggagatcagg gcgcgcagga tggtcgctgc gaaaggcggg gttccggcga
     1201 aagcgacctt caagcgttct ctcagccctg ttgggcgagc agttgctcgc gccactcgcg
     1261 catggcgtcc ttgcgcgact gcctatccgt gcggtcgagg atcaggacgc cgtcgaggtg
     1321 gtcgacctcg tgctgaaata ccctggcgag catgtcgcgg gcctccaggc gcacgggctc
     1381 tcccgaggca tcctggcctg ttacggtaac ggcggtggca cgttccacct cgacgtttat
     1441 gccggggatg gagaggcagc cctcctggcc cggctccttc gtctccgaca cctcagatag
     1501 aacggggtta acgaccacgt aatcctcgtc ctccaccgag gcgacgagaa cccgcttgag
     1561 gcggccgacc tggttggcgg cgaggccaac accatcgttc tcacgcatgg tggcgagcat
     1621 ctcctccgtg aagctcgcga gcgagtcgtc gaacgtctca accggcgcgg cgcgggtctt
     1681 gagcacagga tccccgaatt ttctgatgtc gagcgtcatt tttctagacc tcctgatcca
     1741 gttcgacacg aaccgtgagc ccgtgcgttc tcgccgccgc tctggcggca tccgccgccg
     1801 cgcgggccac cgcgggccgt ctgcgggagc gtagcaggac ccgccatgcg ctacgtcctc
     1861 cggtccgtcc caggggaacc ggggacgaca tctccacttc gggccccagc gtcggccgca
     1921 gccgggattc taccgcaccc aggacggccg attcggaccc gtcgaaggtc agcgaggcga
     1981 agtgtccgaa cggggggtag gcgagggtgc gcagccgcgg gagttcggca gctgcgaacg
     2041 cttcgtagtc gccccgcact ccggcgcgca gggcgtagtg ttcgggaagg cgcgtctgga
     2101 cgatgagcag cccacgggca gcctcccccg cgcgaaagaa caacctgaac gcccgctcca
     2161 cagcgccgac gttactcgcc cccagaaagg cgtcggcgtc tggcaggatc acagcgtccc
     2221 accctccgtc gaggaggcgg ggggcggttc cgacgaccac gggcgcgtcg gcatactccc
     2281 tttcgtcggc ggtgatgagc cctatctgcg ttccgagagc ttccgagagc tggtctcgca
     2341 cccgctcaac gccgagcccg gtcggcctga ggcgttcgga gccgcagttg gagcactctc
     2401 ccagtttctc cctgtggccg cagcgcgtac acaccagtgg cccattcctc ccgtgaagca
     2461 ggaggggcaa ttcgcagttc gggcagagct tcatggtgcc gcagcgactg caggagacag
     2521 tcggcgcgta gcccagacgc cgcaggacga gtcctgccct ccctcccccc tcaacacacc
     2581 ggcggcaggc ttctatcagg gtagaactga gcgtcgcgcc ggaatcccgc atatctacga
     2641 tgcgcaccgc gggccaccgt tcgggccggc tcgcggcgag ctcgcgcaat ccctcccgac
     2701 tgcggacctc ccgagcgtag agcctgaggg aaggtgaagg agagaggaag aacacggccg
     2761 ccccgtctac ccgggagcgc tccagcgcca catcacgcac gtgcaccggg agaccctcgt
     2821 ggccaggccc ggccatgtgt gcgtcgttcg gttcgtccac cacgcaggtc gagcccgggc
     2881 gcgcgaggct aagcagcgcg gccgtccgcg tccccacgac cacgtccaca ttgccgtcgc
     2941 gggcggcccc gtaaacggca gcccgagcac gcccaagccc gccgtgatac tcggcgatag
     3001 tacggccagc gggcaacgcg cggcgcaggt gttccactag cggcccgatt gccctcacct
     3061 ccggaactag tacgagggcc tgtcctccgc cgcgcagcgt ggctgccacg acggcggcca
     3121 cggcatccgg ctcctctcgt gacgtgatcc gccacaggaa agctccgccg ttcctgacgg
     3181 cgcctgccgc cgggcgcgag aacgggcggg caggggcgtc ggcagtaccc tccgcgacca
     3241 ccaggggggc tggctcgggg cgccgcacca gccgtaacgc tcccctgcgg gccagctccc
     3301 gcaacgcggt gcggcttgca ccggtcttcc cgaggagcac agaggtcggg ctggccccgc
     3361 ccacatcttt caaggctacg aacaggtccc gctggcgggg ggcacgtgac aggtccgggt
     3421 cggcggcggc ccggaccacc gcccactcta ccgccctggg ttgcagagca gacggcgaca
     3481 gcacgatgcg tccgctcgcc tcctcccggc gcagcccgtc ggggcccaga acgcgcttga
     3541 gcgtggtacg cgtcacggtg ctgccgggct cccaaggctg gtcctggatc gggcgtagca
     3601 cgagatagcg cccggcgtcg atgccgggcg gcagggcggc cctcaggact gtcgggagcg
     3661 ggacggcggc tgtgtcgcag atggcgaggc acagcctcag gaggtcggga cccggtgaga
     3721 ggtctggtac caccgaaagc acgtcctcac gggcgcggtc ttcctccccc tcgaagccga
     3781 ccacgatgcc aaggcgctgc cgtccggaga gtggtgcgac gaccgcggag cccaacccta
     3841 tctcggccct gtagcgtttc ggtatacggt agctcagggg cggaagcgcg tgcgttggta
     3901 tgagcaggct aacccgcacc gcttgcagtc ggcgggggat atctcgagtc ttctggccag
     3961 ggcctgccgg caactacagg ccggcggcac ggcgaagatc gtcggcccgc gcggtctcct
     4021 cccaggagaa gcctggctcg cggcgtccga aatggccgta ggccgcggtg cgcgcgaaga
     4081 tgggcccgcg caggccaagg tcgcgaataa tggcgccagg tctcaggtcg aagtgttcct
     4141 ggaccagcgc gcccagggtt gccgggtcca cccttgccgt accgaaagtc tcgaccatca
     4201 cgctaacggg gcgggccacg cctatagcgt aggccacctg gaccaggcac ctcccggcca
     4261 gcccggccgc aacgaggttc ttcgccaccc atcttgccgc gtaggacgcc gagcgatcca
     4321 ccttcgtcgc atccttgccg gagaaagcgc cgccaccgtg cggggccgac cccccgtagg
     4381 tgtccacgat gatcttgcgc cccgtgaggc ccgtgtcgcc ctggggtcca cccgtcacga
     4441 accttcccgt ggggtttacc agtacctccg ccgttgcggg gtcgaacagg tcccggggca
     4501 tcacgggcag gatgaccgtc tccatgaggt cgtccctgat ggttgcttcg gcccccgcgc
     4561 ggtgctgggc ggagataagc accttctcca ccgccaccgg cctgtcgccc tcgtagcgta
     4621 cggtcacctg gctcttgcca tccggcctca ggtagtcgag ggcatggctc tttcgaacct
     4681 ccgagaggcg gcgcgtcagg gcgtgagcca gggtaatggg gagcggcatc agctcgggcg
     4741 tctcctcgca ggcgtagccg aacatcatgc cctggtcgcc ggcgccgatc tcgtccaggg
     4801 tctcgtcgtc gttcctgatc tccagcgccc ggtctacccc gacggcgata tcgtcggact
     4861 ggggatctat ggagacgatc acgccgcagg tggcggcatc gaagccgaac ttggccctgg
     4921 tgtagccgat ctccgctatc ttctcgcgca ccagggtcgg gatgtccacg taggtcgagg
     4981 tggtgatctc accagccacg accacgagcc cggtggtgac gagcgtctcg caggccaccc
     5041 ggctcatcgg gtcgtcggcg agcgcggcat cgaggatggc gtcggagatc tggtccgcga
     5101 tcttgtccgg atggccctca gtaacggatt cggaggtaaa gaggtgcgag gtcctgagct
     5161 tctggtcgtc tgccgcgttc caggtggtcg gttcggccgc tctctccacc atcgctacct
     5221 ctgcctttcc atctcgttca tcatggtgtc caaaatgatg ccggctacgg ccgacttgga
     5281 ggccctcggc acgaaatgct cctcctcgcg gcccacgacg tagacctcgt tctcgtccgc
     5341 gccgaacccg acaccctccc cagagatgtc gttgccgacc acgaggtcgg cccccttgcg
     5401 tccgagcttg tcccgcgcgt cctccagggc gtccccgtgc gtcgccgcga atcctaccac
     5461 aaagaggcca gggtaccgcc cggagacgga agcgagtatg tcgtcggtcg gaacgagctc
     5521 caggctcatg gtctccgagc cgcgccggat cttctccctt accggcgagg caggggtgaa
     5581 gtccgagacc gccgcggcca tgacgagcgc gtccgccctc gcggcgagct ccacgacggc
     5641 atcccgcagc tctcccgccg actccacggc tcgggtctcc gtgccgggct cgaccatctc
     5701 cacgttggcg gacaccacgg taacgtccgc gccgcgacgc accgcctcgc gggcgactgc
     5761 cagccccatc tttccagaag aacggtttcc tacgaagcgc acgctgtcga tgggctccct
     5821 ggtgccgccc gccgtgacca gcaagcgcat cccctgcatg ggcccgtcga ggccgcccag
     5881 gagcccggcg acgatgctac ccggatcggc catacctgcg ccggatcctt caacgatgcg
     5941 gtggccgtcc gcgcgcagga gatcgaggtt ctcacggacc gccgggtgct ccgccgtacc
     6001 ggcgtcgagg tcgggggcga tcacgcccag ggctccggct ccgcggtcga ggccgcgggc
     6061 cagccgggcc acggtctcgg atgtcgccgg ggcgtagagc gtcgcctcgg gggtctccga
     6121 tggttcgtcc accagggccg catcgccgaa ggcggcgggg ccgacgaagt ggcgggcccg
     6181 cgggccaagg atgacctcca cctgctggcc cgcctcggcg agcttacgga cgaccgcggg
     6241 tatcgccagc gccccagggg cggctccgac ggagatcagg atcaaggata cgtgaggctc
     6301 tggcccttct aggccacgcc ctcgtcttga ggggcgccgg tgtcgacatc ggatgcctgc
     6361 gggacgccgc caccgctggg cgggcttacg aagtcatccc cgccggccgc accggcctcc
     6421 tcgtccaact cttccaatgg ctgcctgaac gagacgttca gcttgtcctg gcgcaactcc
     6481 tcgatggcta tggtcagggg atgctgggaa cgggtgtgga cctgtggtcc gggaataccg
     6541 agggcgtcgt tcagcccaag ggtagaggtg tactcgttga tctggcgcgc ccgcttggcg
     6601 cttgccacgg ccagaccgta acgtgagtcc accttaccca acagctcatc taccttgaga
     6661 tcgttaagca ccgttgcctc ctcttactat cgattccatg gtttctatca tgtcgttgcg
     6721 ggcctgtgta cggtcggcgt ttacgatctc gtgatcgaac tcgtccgtgg ccgcaacctc
     6781 gccgatggcg gtggcgagcc ggctctccat agcctcctcg gattctgtgg ccctcccggt
     6841 gagcctgcgg cgggtctctt ccagggatgg tgccctgacg aagaccatca cggcgtcagg
     6901 acgcttggtc tttacctgcc tcgcgccctg gagctctatc tccaggatca cggacttgcc
     6961 ttcgcccagg agctcctcga ccttctgttc gggcgtcccg tagaggttac cagaatactc
     7021 ggcccactcc aggaacctgc cctccccgat ccaacgctcg aactcccccc gcgagaggaa
     7081 gacgtagtcc ctgccgttct cctcgccggg gcgggggttg cgcgtcgtcg ccgagacaga
     7141 gtaagcgagc tccggaacgg cctccagtga ggccctgatc aaagtcgatt tcccggctcc
     7201 ggagggaccg gagacgacga ttaaccttcc tcgcacccta gagcccggat gagacggtcc
     7261 ctctgcccgt gcgtgaggcc tacgagcgtc ttggagcggc tgatgccggc atccctcatg
     7321 acgcgcgtaa ccttgatgcg acccatgccc ttgaccgcga ggagcatctc ctcgaccttg
     7381 gcacccttga gctcctcgga aggatccatg agcaggtcgt agatagcgag gctgccgctc
     7441 ttgagatccc gcttggtacc ggcccgggca aagcgcaccc tgttcgcttc ttcgagcgcg
     7501 ttcctgcgct cgtccacggt ccgttctgga gcccttcgca acatcaacga tttagtatac
     7561 atcgccgcaa ccaacccggc caatgcccgg cccaccgctt tc
//
LOCUS       DBNGVD010000185         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3135757, whole genome shotgun sequence.
ACCESSION   DBNGVD010000185 DBNGVD010000000
VERSION     DBNGVD010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3135757"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /gene="nusB"
                     /locus_tag="AC16CM_05170"
     CDS             <1..263
                     /gene="nusB"
                     /locus_tag="AC16CM_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3139253.1"
                     /translation="EQEGLDAKLAEVSEGWPLHRMSAIDRTILRLALYEMLYVDDVPP
                     EVAVNEAVELATGYSGEDAPAFVGGVLRGASLRQHGEVGQGG"
     gene            256..1161
                     /locus_tag="AC16CM_05175"
     CDS             256..1161
                     /locus_tag="AC16CM_05175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3139254.1"
                     /translation="MGEAGMNGSGVRWESHRRLFEEYLGGLRFTEEARLEGLEEAMRY
                     SMLAGGKRVRPTLCMEVAWIFGADPALVLPSAAAIELIHTYSLIHDDLPAMDNDDFRR
                     GMPTAHKKFGEAMAILAGDAFFGEALTLITVHQQASCEQQLDVVRELADSTGVNGMVG
                     GQIMDMDRTGQGEVTDPETLYMIHKYKTGALIKSSARIGAILAGATREEQAAVSEYAS
                     ELGLCFQIVDDVLNATSTREELGKSAGSDAEQGKATFVGVFGLDGAKREADGAAKRAV
                     GALSMVDGATSGLRELAYFVRHRES"
     gene            1171..1974
                     /locus_tag="AC16CM_05180"
     CDS             1171..1974
                     /locus_tag="AC16CM_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3139255.1"
                     /translation="MNGSPKGSKQRLDAMLVERGLAESRSRAEALILAGSVRVGDRVV
                     TKAGSRIAPDEEIHVQDAKRFVSRAGEKLEAAIREFDVQVKGRDCLDAGASTGGFTDV
                     LLQRGAGRVLAVDVGYGQLAWSLRNDPRVTVMERTNVRHLSGGELPFGPDLLVADLSF
                     ISLAVALGRLFATTLTLREAVLLVKPQFEAGPEAVGRGGLVRDPAVHAAVVLDVARTF
                     GELGFGAVGVMRSTVPGRKSGNVEYPVHLVRDAVSSLDGSKVGEVVAGG"
     gene            1967..>2424
                     /locus_tag="AC16CM_05185"
     CDS             1967..>2424
                     /locus_tag="AC16CM_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3139256.1"
                     /translation="MAEDMKAGSIRRVGIVANPKAGDGYVDRLVAVLREHRVEVAEVR
                     GDDQPDGHRDMDLVFVLGGDGTMLRASRIYPDNVLLGVNLGKVGFMSGMLPEDLEKGV
                     EKVLAGGLEVQEYKMLDVLVEGEEPSVAANDAVLIKKRPEQLISVDVSIAG"
BASE COUNT          415 a          739 c          881 g          389 t
ORIGIN      
        1 aggagcagga agggctggat gccaagctcg cagaggtctc tgaagggtgg ccccttcacc
       61 gcatgagcgc catagaccga accatcctgc gcctcgcgct ctacgagatg ctctacgtag
      121 atgatgtgcc gcccgaggtg gcggtcaacg aggccgtgga gcttgctacc gggtactcgg
      181 gggaggacgc cccggccttc gtggggggcg tgttgcgggg cgctagcctg cgccagcacg
      241 gtgaggtggg gcagggtggg tgaggccggc atgaacggga gcggggtccg ctgggagtcg
      301 caccggcggc tcttcgagga gtacctgggg ggcctcaggt tcaccgagga ggccaggctg
      361 gaggggctcg aagaggccat gaggtactcc atgctcgctg gcggaaagcg cgtcaggccg
      421 accctgtgca tggaggttgc ctggatcttc ggcgccgatc ctgccctggt gttgccctcc
      481 gccgcggcca tagagctcat ccacacctac tccctcatac acgacgatct gccggccatg
      541 gataacgacg acttcaggcg cggaatgccg accgcccaca agaagttcgg cgaggccatg
      601 gctatcctcg ccggcgacgc cttcttcggc gaggccctca cgctcatcac cgtccaccag
      661 caggctagct gcgagcagca gctcgatgtg gtgcgcgagc tcgccgactc cacgggcgtc
      721 aacggtatgg ttggcggcca gatcatggat atggacagaa ccggtcaggg ggaggttacc
      781 gaccccgaga cgctctacat gatccacaag tacaagacgg gggccctgat caaatcctcg
      841 gcccgcatcg gcgccatcct ggccggcgcg acccgcgagg aacaggcggc cgtctccgag
      901 tacgcctcgg agctggggct gtgcttccag atcgtggacg acgtcctgaa cgccacctcc
      961 acacgcgagg agctcggaaa gagcgccgga tccgacgccg agcagggcaa ggcgacgttc
     1021 gtcggcgtgt ttggtctgga tggggcaaag agggaggcag acggcgcagc caagagggcc
     1081 gtcggggcgc tctccatggt ggacggcgcc acctcgggcc tgcgggagct ggcatacttc
     1141 gtgcgccaca gggagagcta gccgtcgggc atgaacggta gcccgaaagg gagcaagcag
     1201 cggctcgacg ccatgctcgt cgaacggggc ctcgccgaga gcaggagccg ggcggaggcc
     1261 ctgatcctcg ccggatccgt gcgcgtgggt gaccgcgtgg tgacgaaggc cggatcgcga
     1321 atcgcacccg acgaagagat ccacgtgcag gacgcgaaac gctttgtctc aagggccggc
     1381 gagaagctgg aagctgccat cagggagttc gatgtgcagg tgaagggccg cgattgcctc
     1441 gacgccggcg cctcgaccgg tggcttcacc gacgttctgc ttcagcgggg ggccggtagg
     1501 gtgctcgccg tggatgttgg ctacggccag ctcgcgtggt ccctgcgcaa cgacccgcgg
     1561 gttaccgtta tggaacgtac caacgtcagg cacctctcgg gcggggagct gccgttcggt
     1621 ccagatctgc tcgtggccga cctgtctttc atctcgctcg ctgtcgccct cgggcgcctc
     1681 ttcgcgacca ccctgaccct gcgcgaggcc gtgttgctgg tcaagcccca gttcgaggcc
     1741 gggccggagg ccgtcggccg gggggggctc gtgagggacc ccgcagtaca cgccgccgtt
     1801 gtccttgatg tcgcccggac tttcggcgag ctcggcttcg gcgccgtcgg ggttatgcgg
     1861 agcaccgtac ccggacgaaa atccgggaac gtagagtatc cggttcatct ggtccgggac
     1921 gcggtatcct ccttggacgg gtctaaagtg ggggaggtgg tcgccggtgg ctgaggacat
     1981 gaaggctggg tcgatccgcc gcgtggggat agtcgccaac ccgaaggcgg gcgacggcta
     2041 cgtcgacagg ctcgtggcgg tcctgcgcga gcaccgggta gaggtcgccg aggtcagggg
     2101 cgacgatcaa cccgacggcc acagggacat ggacctggtg ttcgtactcg gtggcgacgg
     2161 aaccatgctc agggcgtccc ggatctaccc ggataacgtg ctcctcggcg tcaacctggg
     2221 caaggtcggc ttcatgtcgg ggatgctgcc cgaagacctg gagaagggcg tcgagaaggt
     2281 gctcgccggc ggtctcgaag tgcaggaata caagatgctg gacgtgctgg tggagggcga
     2341 ggagccgagc gtggccgcaa acgacgccgt gctcatcaag aagcgtcccg agcagctcat
     2401 ctcggtggat gtctccatag ccgg
//
LOCUS       DBNGVD010000186         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1860185, whole genome shotgun sequence.
ACCESSION   DBNGVD010000186 DBNGVD010000000
VERSION     DBNGVD010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1860185"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2001
                     /locus_tag="AC16CM_05190"
     CDS             58..2001
                     /locus_tag="AC16CM_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3139257.1"
                     /translation="MADPTVSRGRAGHRIRRWLLQDRIEEVKGPEAREGEQTHAWWQV
                     VCLTGVDYFSTLGYIPGIAALAAGALSPIATLLIVLLTLLGMLPMYRVVARESPSGQG
                     SIAMLETLLSFWRGKLFVLCLLGFVATSWIITITLSASDATAHFTENPLVPQFFQDYE
                     VAITIVLLALLGAVFMKGFREAIGVAVSIVGVYLALNLVVVGTGFYEILSNPQTLAGW
                     QDALFTSYGSPFTMLAVSFLVFPQLALGLSGFETGVSMMPLVRGDEDDDPERPEGRIR
                     NTHKMLFTAALIMSFYLLTTSFVTSVLISPEDFEQGASDRALAFLAHEFLGNVFGTIY
                     DISTMTILWFAGASAMAGLLNIVPRYLPRYGMSPEWGRAVRPLVLVYTLISVVVVIIF
                     AADVNEQAGAYATGILAMMTSAAFAVTLTVARHGHRIGAVLFGLTTLVFVYALVANVY
                     RRPDGITIALFFIGAIVVISLVSRVQRSTELRQERIDVDETAESFIREAAERGEIHLV
                     AHRRRNGTKEEYERKEREQREDNHIPSEVPILFLEVQVDDASEFMDVLEVRGVEVGGH
                     KVLRSVSSVVPNAIAAFLLHLRDKTGHTPHCYFGWTEGNPIIYLIRYILFGEGDTAPV
                     THEVLREAEPNPERRPAIHVGGR"
     gene            1998..2672
                     /locus_tag="AC16CM_05195"
     CDS             1998..2672
                     /locus_tag="AC16CM_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3139258.1"
                     /translation="MKRQFLVVGLGRLGISMVATLDSLGHEVLGIDKRDDVIQEVADD
                     LPRVELVAADATDERAMRGLNVNGFDAAAVMIGENHIEAGILATATLKEAGVPRIIAR
                     ATGGVHARVLERIGADRVTRPELELGQQLARTMASPAILDYVDFGEDEALIEARVPPE
                     WLDKSLGELALSHEKGLTVVALKPQGGPGILPHGDTVLHSGDVLVVGGTKEALDRSPL
                     IRPSDR"
     gene            2685..>2797
                     /locus_tag="AC16CM_05200"
     CDS             2685..>2797
                     /locus_tag="AC16CM_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3139259.1"
                     /translation="MRTVIIGAGEVGLSTARLLSHEGHSVVLVEKDEKLVE"
BASE COUNT          500 a          881 c          904 g          512 t
ORIGIN      
        1 ctatcgtcca acccaggcac gccggtccgg ggcccacaaa ctcagagggg caacgtagtg
       61 gccgacccaa cggtttcaag aggtcgggcc gggcatcgga tccggcgctg gcttctgcag
      121 gaccgcatcg aggaagtcaa gggaccggag gcccgcgagg gcgaacagac ccacgcctgg
      181 tggcaggtgg tgtgcctcac gggcgtagac tacttctcca cccttggtta cataccgggc
      241 atagccgcgc tcgcggcagg cgcactctcg cccatagcca cgctcctgat cgtgctcctg
      301 accctactgg ggatgctgcc catgtacagg gtcgtggcaa gggagagccc aagcggccag
      361 ggctccatcg ccatgctcga gaccctgctc tcgttctggc gaggcaagct cttcgtgctc
      421 tgcctgctcg gcttcgtggc cacttcctgg atcatcacga tcacgctctc cgcctcggat
      481 gcgacggccc actttaccga gaacccgctg gtgccccagt tcttccagga ttacgaggtg
      541 gcgatcacca tcgtgttact ggccctgctt ggtgcagtat tcatgaaggg attcagggag
      601 gccatcggcg tggcggtctc catagttggg gtgtatctgg cgctgaacct ggtggtcgtg
      661 ggcaccggct tctacgagat actctccaac ccccagacgc tcgccgggtg gcaagacgcg
      721 ctgttcacga gctacggcag cccgttcacc atgctggctg tctcgtttct ggtatttccg
      781 cagctcgctc ttggcctatc cggcttcgag accggcgtca gcatgatgcc gctcgtgcgc
      841 ggggatgagg acgacgaccc ggaacgcccc gaaggccgta tccgcaacac ccacaagatg
      901 ctctttaccg ctgccctgat catgagcttc tacctcctca cgaccagctt cgtgacctct
      961 gtgctgatct cccccgagga tttcgagcag ggcgcaagcg accgcgccct cgctttcctg
     1021 gcccacgagt tcctggggaa tgttttcggc accatctacg acataagcac aatgacgata
     1081 ctgtggttcg ccggtgcctc ggcgatggcc ggtttgctaa acatcgtgcc ccgctatctg
     1141 ccccgctacg gcatgtctcc cgagtggggc agggcggtca ggccgctggt tctggtgtac
     1201 accctgatct ccgtggtagt cgtgatcatc ttcgcggctg atgtgaacga gcaggccggg
     1261 gcttacgcga cgggcattct ggctatgatg acttcagcgg cgttcgccgt gacccttacg
     1321 gtggcccgtc acgggcacag gataggggcg gtgctcttcg ggctcaccac cctcgtcttc
     1381 gtatacgcgc tcgtggcaaa cgtctacagg cggccagacg gcatcaccat cgcgctcttc
     1441 ttcatcgggg cgatcgtcgt gatctcgctc gtctcccgcg tgcagcgttc tacggagttg
     1501 cgtcaggaga ggatagacgt agacgagacc gccgagagct ttatccggga ggccgccgag
     1561 cggggcgaga tccacctggt ggcccaccgc cgccgcaacg gcaccaagga agagtacgag
     1621 aggaaagaga gggagcagag ggaggacaat cacatcccat cagaggtgcc tatcctgttc
     1681 ctggaggtac aggtcgacga cgcctccgag ttcatggacg tgctagaggt gaggggtgtt
     1741 gaggtcggcg gtcacaaggt gcttcgttcc gtgagctcgg tggtcccaaa cgccatcgct
     1801 gcctttctcc tgcacctgag ggataagacg gggcacacac cgcactgcta cttcggctgg
     1861 accgagggca accccatcat ctacctgatc cggtacatcc tcttcggcga aggtgatacg
     1921 gctcccgtca cccacgaggt cttgagggaa gccgagccga acccggagag gcgccccgcc
     1981 atacacgtgg gtggacgatg aagcggcagt tcctggtcgt tggtctcggg cggctcggca
     2041 tctcgatggt cgccacgctc gactccctgg gccacgaggt cctcggcata gacaagcgcg
     2101 acgacgtgat ccaggaggtc gccgacgacc tgccccgtgt ggagctcgtg gccgctgacg
     2161 ccaccgacga gagggccatg cgcggcctga acgtcaacgg cttcgacgcc gccgccgtaa
     2221 tgatcggcga gaaccacatc gaggccggca ttctggcgac ggccaccctg aaggaggccg
     2281 gggtacccag gatcattgcc cgggcaacgg gaggggtcca cgccagggtg ctcgaacgca
     2341 tcggcgccga cagggttaca aggcccgagt tggagctggg ccagcagctc gccagaacga
     2401 tggcctcacc ggccatcctc gactacgtcg acttcgggga ggatgaggcc ctgatcgagg
     2461 cgagggtgcc tccggagtgg ctcgacaagt cgctcggcga gctggcgctc tcgcacgaga
     2521 aaggcctcac ggtggtggca ctcaaacccc aggggggtcc cggcatcctg ccccacggtg
     2581 ataccgtatt gcactcgggt gacgttctcg tcgtcggggg tacgaaagag gccctggaca
     2641 ggtcaccgct tatcaggccg tccgatcgtt aggaggcacc cgggatgagg acggtcataa
     2701 taggggcagg ggaggtgggg ctgagcaccg cccgcctgct ctcgcacgag ggccacagcg
     2761 tcgtcctggt ggagaaagac gagaagcttg tggagaa
//
LOCUS       DBNGVD010000187         3905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4229546, whole genome shotgun sequence.
ACCESSION   DBNGVD010000187 DBNGVD010000000
VERSION     DBNGVD010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4229546"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="AC16CM_05205"
     CDS             <1..1065
                     /locus_tag="AC16CM_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3139260.1"
                     /translation="WRSLSQWIGGIGIIVLFVAIAPQAGFGATQMYTAEAATPLQERI
                     APRIRDTVKVLALVYGGLTIGGVVVLHLAGMGTFDAFNYAFSTVSTGGFATDADSVAS
                     FDSWAVDLALTAGMVLAGTNFAIYYVAARHGLGRAVGNAELVTYLGVILVCTVLVTVD
                     LYTSAYPGSLAVVFREALFQSASLTTGTAFSTTDWSAWPPFSVALLVVVMAVGGCAGS
                     TAGGIKAIRVYLLWRNAAQEMFRLIHPRAVTPLVLGDRVLPERTRLGLLSFFFIYVAT
                     MIIGTLLMALHNVPLGQAFGSVFACVNITGSFPGAAGTPGFYEALPATGKTILAALML
                     LGRLELFTVLVLLSRSFWRA"
     gene            1329..2480
                     /gene="sucC"
                     /locus_tag="AC16CM_05210"
     CDS             1329..2480
                     /gene="sucC"
                     /locus_tag="AC16CM_05210"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3139261.1"
                     /translation="MDLYEYQGKELLRNYGLETLPGIVAGTPEEAARAAEALGGTVAI
                     KAQVLIGGRGKAGGIKVVSNPREAREAADQILGMDIRGHTVRRVYVEGGAEIEREFYL
                     SITVDRTAKRPLILFSTEGGVEIEEVAETNPDAIVRLHVDPLVGLLPYQVREVTFAAG
                     LEGDAAKGVGRTLTSLYEAFEGLDASLVEINPLVLTSEGHVLTLDAKVTVDNSSLYRH
                     GDIADLHDVEAADVQEQRAQEVGLQYVKLDGNVGILGNGAGLVMSTLDVVAQAGGDPA
                     NFCDVGGGADAEKIAEALDIVTSNEQVESVLFNIFGGITRGDEVARGLLSAIERTGIT
                     LPIVVRLDGTNAEEGRTILAENTPENVHAEETMLEAARKAVELARDGGR"
     gene            2484..3359
                     /gene="sucD"
                     /locus_tag="AC16CM_05215"
     CDS             2484..3359
                     /gene="sucD"
                     /locus_tag="AC16CM_05215"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3139262.1"
                     /translation="MAILVDEGTRLLVQGITGREGAFHTGRMLSSGTAVVAGVTPGKG
                     GQDYEGVPIFNTVDEAISETGANASVIFVPPPFAADAIFESLAGGLSTVCCITEGIPV
                     HDMMRVAGYIEHKDATLVGPNCPGVLSPGKSNVSIMPGDIFMPGKVGVVSRSGTLTYQ
                     IVNELTQRQIGQSTAVGIGGDPIIGTDFIEILSLFEADPETECAVMIGEIGGNAEQLA
                     AEYVRGEMKTPVVAYIAGFTAPEGKTMGHAGAIVSGGDSTAEAKSEALREAGIRAATN
                     PSEVAELVADALGRS"
     gene            3489..>3905
                     /locus_tag="AC16CM_05220"
     CDS             3489..>3905
                     /locus_tag="AC16CM_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HIR3139263.1"
                     /translation="MADPTVSIGRAGHRIRRWLLQDRIEEVKGPEAREGEKTHAWWQV
                     VCLTGVDYFSTLGYIPGIAALAAGALSPIATLLIVLLTLLGMLPMYRVVARESPRGQG
                     SIAMLETLLSFWRGKLFVLCLLGFVATSWIITITLSA"
BASE COUNT          675 a         1216 c         1308 g          706 t
ORIGIN      
        1 tggcgcagcc tgagccagtg gatcgggggc atagggataa tcgtgctgtt cgtggccatc
       61 gcgccgcagg cgggtttcgg cgcaacccag atgtacacag cagaggcggc tacacccttg
      121 caggagcgca tcgcccctcg cattcgggac accgtcaagg ttctggccct cgtctacggc
      181 gggctcacaa tagggggggt cgtggtcctc cacctggccg ggatgggtac cttcgacgcg
      241 ttcaactacg cgttcagcac cgtctccacc ggcggcttcg ccaccgacgc tgactccgtg
      301 gcctccttcg attcctgggc cgtggatttg gccctcacgg cgggcatggt actcgccggc
      361 acgaacttcg ccatctacta cgtggcggcc aggcacgggc tgggacgcgc cgtcggcaac
      421 gccgagctcg taacctatct tggggtgatc ctggtgtgca cggtgctcgt aaccgtcgac
      481 ctctacacct ctgcctaccc gggctcgctg gccgtggtgt tccgcgaggc cctcttccag
      541 agcgctagcc tgaccacggg aacggccttc agcacgacgg actggagtgc ctggccccca
      601 ttttcggtgg cgctgctggt cgtcgtgatg gccgttgggg gctgtgcagg ttcgaccgct
      661 gggggcataa aagccatccg ggtctaccta ctgtggcgca acgccgcgca ggagatgttt
      721 cgcttgattc atcctcgggc ggtgacgccg ctggttcttg gcgacagggt actgccagag
      781 cggacccggc tggggctcct gagcttcttc ttcatctacg tggcaaccat gatcataggc
      841 accctcctta tggcccttca caacgtcccg ttgggtcaag ctttcgggtc tgtgttcgcg
      901 tgcgtgaaca taacaggctc gttccccggc gcggctggaa cccccgggtt ctacgaagcg
      961 ctgcccgcta caggcaagac catcctcgcc gccctgatgc tcctgggtcg actcgaactg
     1021 ttcaccgtac tcgtgctgct cagccgctcc ttctggcggg cttagcttct agcttctagc
     1081 ttctagcttt tagctcttag ctttcagcgg tcagctttca gcctgtagtt cttgttggcg
     1141 ggtgggcgat agttgggttg gagcggtgcc ggggtgggcc tgtgggtggc gtgatggtgg
     1201 ttgcagggga aatatgctcg tgcgtcagcg gagccttgtt tgggggcctc tcgttgtttt
     1261 tcgcgtgcgc ggtatgggaa tctgtataat tctggaccgt cgtagcgggc ttcgcggggg
     1321 tttccaactt ggatctctat gagtatcagg gcaaagaatt gttgagaaac tacggcctcg
     1381 agacgctgcc aggcatcgtg gccggcacgc cggaggaggc cgcgagggcc gcagaggccc
     1441 tcgggggcac ggtagccatc aaggcccagg ttctgatcgg cggccggggc aaggccggcg
     1501 gcatcaaggt cgtgagcaac cccagggagg ccagggaggc ggccgatcag atccttggca
     1561 tggacatccg gggccacacg gtcaggcgcg tctacgtcga aggtggggct gaaatcgaga
     1621 gggagttcta cctctcgatc acggtggacc ggacggcgaa acggcccctg atcctgttct
     1681 ccaccgaggg cggcgtagag atagaggagg tagccgagac aaacccggat gccatagtcc
     1741 ggcttcacgt cgacccgctg gtcggcctgc ttccctacca ggtgcgcgag gtcaccttcg
     1801 ccgctggtct ggagggcgac gccgccaagg gggttggaag gacgctaaca agcctatatg
     1861 aggccttcga gggcctcgac gctagcctgg tcgagatcaa ccccctcgtc cttacgagcg
     1921 aggggcacgt tctgacgctg gacgcgaagg ttacggtgga caactcgagc ctgtaccgac
     1981 atggcgacat agccgacctg catgacgtgg aggccgccga cgtgcaggag cagcgggcgc
     2041 aggaggttgg cctgcagtac gtcaaactgg acggcaacgt gggcatcctg ggcaacggcg
     2101 ctgggctcgt gatgagcacg ctcgacgtgg tagcccaggc cggaggcgat cccgccaact
     2161 tctgcgacgt gggcggcggt gcggacgcgg agaagatcgc cgaggcgctc gatatcgtca
     2221 cctccaacga gcaggtcgag agcgtgctct tcaacatctt cggcggcata acccgcggcg
     2281 acgaggtggc gcgtggcctg ctatcagcaa tcgaacggac cggcatcacc cttcccatcg
     2341 tcgtgcgcct cgacggcacg aacgccgagg aggggcgcac gatactggcc gagaacacgc
     2401 cggagaacgt tcacgcggaa gagaccatgc tggaggcggc ccgcaaggct gtggagctcg
     2461 ctagagacgg gggacgataa aggatggcga ttctcgtaga cgaagggacg aggcttctcg
     2521 tccagggcat aacaggcagg gagggcgcct tccacaccgg aaggatgctc tcgagcggga
     2581 cagcggtggt cgcgggtgtt acgcccggca agggcggtca ggactacgag ggtgtaccca
     2641 tcttcaacac cgtcgatgag gccatcagcg agaccggggc aaacgccagc gtgatcttcg
     2701 tgcccccgcc tttcgcggct gacgccatct tcgagtcgct cgccggcggg ctctccacgg
     2761 tctgctgcat cacggagggc atacccgtgc acgacatgat gcgggtagca ggctacatcg
     2821 agcacaagga cgcgacgctc gtcgggccga actgtcccgg cgtcctctcg cccggcaagt
     2881 ccaacgtctc catcatgccg ggcgacatct tcatgcctgg caaagtcggc gtggtcagcc
     2941 gcagcggcac gctcacctac cagatcgtca acgagctaac gcagcgccag atcgggcaga
     3001 gcacggccgt gggcatcggg ggcgacccca taatcggaac ggactttatc gagatcctta
     3061 gcctcttcga ggcggacccc gagacggagt gcgccgtgat gattggcgag atcgggggca
     3121 acgccgagca gctggccgcc gagtacgtca ggggcgagat gaagaccccc gtcgtggcct
     3181 acatcgcggg cttcaccgct ccggagggca agaccatggg gcacgcagga gccatcgtct
     3241 ccggtggaga cagcactgcc gaggccaaga gcgaggccct gagggaggct ggcatcaggg
     3301 cggcgacgaa cccatcggag gtcgccgagc tggtcgccga cgcgctggga cggtcgtgag
     3361 ggactagccc ccgcagggtc cccacccgta ttcctcccat accttatact ccctaggctg
     3421 accatgaacg tctatcgtcc aacccaggca cgccggtccg gggcccacaa actcagaggg
     3481 gcaacgtagt ggccgaccca acggtttcga taggtcgggc cgggcaccgg atccggcgct
     3541 ggcttctgca ggaccgaatc gaggaggtca aggggccgga ggcccgcgag ggcgaaaaga
     3601 cccacgcctg gtggcaggtg gtgtgcctca cgggcgtcga ctacttttct acccttggct
     3661 acatacccgg catagccgcg cttgcggcgg gcgccctctc gcccattgcc acgctcctga
     3721 tcgtgctcct gaccctcctc gggatgctgc ccatgtacag ggtcgtggca agagagagcc
     3781 cgcgcgggca gggctccatc gccatgctcg agaccctgct ctcgttctgg cgaggcaagc
     3841 tcttcgtgct ctgcctgctc ggcttcgtgg ccacttcctg gatcatcacg atcacgctct
     3901 ccgcc
//
LOCUS       DBNGVD010000188         2765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_768083, whole genome shotgun sequence.
ACCESSION   DBNGVD010000188 DBNGVD010000000
VERSION     DBNGVD010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_768083"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16CM_05225"
     CDS             <1..462
                     /locus_tag="AC16CM_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3139264.1"
                     /translation="PVAANRGASGIDGTVATAAGFARGLGRPVTLLIGDLALLYDLNS
                     LAMLRDVPVVVVVLNNDGGGIFSFLPVAERKEFFEPYFGTPQGVGFESAAEMFGLHYE
                     RPETTGEFVEVYRGACARGSSTLIEVRTDRAENLALHRRILGDVAGFVGRG"
     gene            470..937
                     /locus_tag="AC16CM_05230"
     CDS             470..937
                     /locus_tag="AC16CM_05230"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3139265.1"
                     /translation="MAHGRVTETIPAQARLVFDLVHDYPKRLEWDTLLSAAYLDDGYT
                     EAAKGATSVCVGKWYLGGIALKTFYVVFDRPGVAAVKMVNRPPFFDSWGASLRHEDTG
                     QDSSRITYAFHFTTKPEVLRFVLDPLMGVVFEFEVKRRLRALKRHFSRPTYPK"
     gene            934..1749
                     /gene="menH"
                     /locus_tag="AC16CM_05235"
     CDS             934..1749
                     /gene="menH"
                     /locus_tag="AC16CM_05235"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HIR3139266.1"
                     /translation="MTHQSGLAYEVSGKRELPGVLLLHGFMGSSADWRGVEAVLGDRA
                     FCIAPDLPGHGASVGLAPVAYTMEETARAVVRILDELGTRRPVVVGYSMGGRLALYLA
                     LRCPDRCAAIFLESASPGLEGDEERAVRRDADEERARWLESGDFGGFLRAWYRQPLFA
                     SLARDEGLLRRIIEARRHNDPRELARSLRGIGTGSQPSLWEELGGLSLPALAVAGERD
                     ARYAGISSRMASISPKVEPVVIPDVGHNLHDEAPEEYAALLGGFLDRITPGAG"
     gene            complement(1783..2553)
                     /locus_tag="AC16CM_05240"
     CDS             complement(1783..2553)
                     /locus_tag="AC16CM_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3139267.1"
                     /translation="MMYLGLSLAVVMGVALGLLGGGGSILAVPILVYALGFGAKEAIA
                     VSLAVVGLTSLLAAAEHWREGHVRFRLALVFGLVTAVGAYLGAYLAGFVDGSFQLSLF
                     AVVMLTAAIFMYRNGRSEDHEKRDHSGERVMGILARFALPGIGVGTLTGLVGVGGGFL
                     IVPSLVLLGGIPMEAAIGTSLLVIAMNSFAGLAGYLGEATFPLGLVSLFSALAVAGTF
                     AGTFLVRFVPEGALKRWFAVLLVLMALFILYENWHVIL"
     gene            complement(2563..>2765)
                     /locus_tag="AC16CM_05245"
     CDS             complement(2563..>2765)
                     /locus_tag="AC16CM_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3139268.1"
                     /translation="QRLRDGIPDHIRDTDGKPLAVVCGSGVRSALAASLLRRSGVVGV
                     VHVADGGVPMLASEGVELVAGT"
BASE COUNT          498 a          900 c          932 g          435 t
ORIGIN      
        1 ccagtcgccg ccaacagggg cgcgagcggc atcgacggca ccgtcgccac cgcggcgggc
       61 ttcgcgcggg gtctcggaag gcccgttacc ctcctcatag gggatctggc gttgctgtac
      121 gatctcaact ccctcgccat gctgcgcgac gtgcccgtcg tcgtggtggt gctcaacaac
      181 gatggtgggg ggatcttctc cttcctcccc gtcgccgaac gcaaggagtt cttcgagcct
      241 tatttcggca cgccgcaggg cgtgggcttc gaatccgccg ccgaaatgtt cgggctgcac
      301 tacgagcgtc cggagacgac cggagagttc gtcgaggtct accgcggggc ctgcgcccgt
      361 ggttcctcca ccctgatcga agtccgcacg gaccgtgcgg agaaccttgc cttgcaccgc
      421 aggatactcg gggacgtagc gggtttcgta gggagaggat gatacgcgcg tggcgcacgg
      481 tagggtgacg gagacgatac cggcccaggc ccggctggtc ttcgatctgg tgcacgatta
      541 tcccaagcgc ctggagtggg atacgctgct gagcgccgcc tacctcgacg acggctacac
      601 ggaggccgcg aagggtgcga cctcggtatg cgttggaaag tggtacctgg gcggaatcgc
      661 gctcaagacg ttttacgtgg tcttcgaccg ccccggggtc gcggccgtca agatggtgaa
      721 ccgaccgccc ttcttcgatt cgtggggagc gtcgctccgg cacgaggata cgggccagga
      781 ctcctcacgc atcacctacg cgttccactt cacgacgaaa cccgaggtcc tgcgtttcgt
      841 cctcgacccg ctgatggggg tggtcttcga gttcgaggtg aagagacggt tgcgggccct
      901 gaagcgtcat ttcagccgac ctacctaccc gaagtgacgc accagagcgg gctggcctac
      961 gaggtctcgg gcaaaaggga gctcccgggc gttctcttgc tgcacggttt catgggatct
     1021 tccgcggact ggcggggcgt ggaggccgtc ctcggagacc gggcattctg catagccccg
     1081 gatctaccgg gacacggagc ctctgttgga ctcgcgcccg tggcctacac catggaggaa
     1141 acggcgcggg cggtagtgag aatcctcgac gagctcggga ccaggcgtcc ggtcgtggtc
     1201 ggttactcga tgggcggacg gctggccctg tacctggcgc tacgctgtcc tgatcggtgc
     1261 gccgccatct tcctcgaatc ggcctcaccg ggcctcgaag gcgacgaaga gcgggcggtg
     1321 cgaagagatg cagacgaaga gagggccagg tggctggaat ccggagactt cggggggttc
     1381 ctgagggcct ggtatcgtca gccgctcttc gcctccctgg cgcgggacga aggattgctc
     1441 cggaggatca tcgaggcgcg gcggcacaac gatccgcggg agctagcgcg ctcgctccgg
     1501 ggtatcggga cgggaagcca gccttcgctg tgggaggagc tcgggggact ctccttaccg
     1561 gcgctcgccg tcgccggaga acgggatgcg aggtacgctg gcatctcctc tcgcatggcc
     1621 agcatcagcc cgaaagtcga gcccgtggtg ataccagacg tgggacacaa cctgcacgac
     1681 gaggcccctg aggagtacgc agccctgctc ggaggcttcc tggaccggat tacccctggc
     1741 gctggttgag accgaaccgg tacgtcccgg agccgtcgtt gctcagagaa tcacgtgcca
     1801 gttctcgtag agtatgaaga gcgccatcaa gacaagcagt acggcgaacc acctcttgag
     1861 ggccccttct gggacgaacc gcacgaggaa ggtcccggcg aaggtcccgg cgaccgccag
     1921 cgcggaaaac agtgagacga gacccaacgg gaacgtcgcc tcgcccaggt acccggccag
     1981 accggcgaag gagttcatcg cgatgaccag caaagaggtg ccgatcgcag cctccatggg
     2041 gatcccgccg agtagcacca gggacggcac gatcaggaac cccccgccga cgccaacgag
     2101 accggtaagg gtcccgacgc cgatccccgg cagggcgaag cgggccagaa tccccatgac
     2161 cctctcgccg gaatggtctc tcttttcgtg gtcttctgat ctgccgttgc ggtacatgaa
     2221 gatggccgcg gtcagcatca ccacggcgaa gagcgagagt tggaaagatc cgtccacgaa
     2281 gccggccagg tacgccccga gataagcgcc aaccgcggtg acgagcccga acacgagcgc
     2341 gagacgaaac ctcacgtggc cctcgcgcca gtgctcggcg gcggcgagga ggctagttag
     2401 cccgaccacg gccaggctca cggcgatggc ctccttcgcg ccgaaaccaa gcgcatacac
     2461 gaggatcggc acggcgagta tggatccgcc gccaccgagt agcccgagcg ccacccccat
     2521 cacgacggcg agcgacaggc ccagatacat catcccggtg cctcaggttc ccgcgacgag
     2581 ctcgacgccc tcgctggcga gcatcgggac gccgccgtca gccacgtgta ccacgcccac
     2641 cacgcccgac ctccgaagca gcgaggcggc cagggcgctg cgcacgccgc tgccgcagac
     2701 caccgccagg ggcttgccgt ccgtgtcgcg gatatggtcg gggatcccgt cgcggagccg
     2761 ctggt
//
LOCUS       DBNGVD010000189         4045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1314954, whole genome shotgun sequence.
ACCESSION   DBNGVD010000189 DBNGVD010000000
VERSION     DBNGVD010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1314954"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="AC16CM_05250"
     CDS             complement(<1..1010)
                     /locus_tag="AC16CM_05250"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3139269.1"
                     /translation="MKAMALAAGKGTRLFPLTGEIPKPMAPVVNTPIIEHIFELLARH
                     GIEEVRVNVHYLADALLGAYGEESKVDGMRVYLEREDKLLGTAGGVKRLADRFDETFV
                     VISGDALTDVNITELVERHKERGALATIALRRVFDTSEFGVVDVDDEGNIEGFQEKPD
                     PGEAISTLANTGIYVFEPRALDYIPEGAFFDFAENVFPKLLENGEKFVGYEEDFYWSD
                     IGTLEAYRQAQYDVLSGKVDVRIPGEKRGESFWVGEDAQIHPSAEIEGQVLVGRDAVV
                     GRGVKLLEDVTIGTDCWIRPNATIKRSILLPGASVGDGAYLEDCIVGHHYDVRPQETI
                     RGG"
     gene            1061..1954
                     /gene="murB"
                     /locus_tag="AC16CM_05255"
     CDS             1061..1954
                     /gene="murB"
                     /locus_tag="AC16CM_05255"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3139270.1"
                     /translation="MHTGTLQRLFPSAKLDEPLKRYTAWKIGGPADALLEPGTVEDLI
                     SATQIAREHDIPVTILGGGTNVLVRDGGIRGLTIRLAKSLRNVEIEGTTVTADAGVLY
                     PVLANTTAGRGLAGLQFATGIPGTVGGAVYMNAGAYGSETAEVLDWADVLRDGEVIRM
                     ENRDLDLSYRYSALHDHPGWIVLRAGYTLVPGDPTELKARIKEFRAQRMSGSPNRPSC
                     GSTFKRPQGDFPGRVIEAAGLKGTRVGNIEVSPVHANYLVNLGGGTAEDAIALIELVR
                     KTVQEKLGIELESEVRIVGEP"
     gene            complement(2033..2620)
                     /locus_tag="AC16CM_05260"
     CDS             complement(2033..2620)
                     /locus_tag="AC16CM_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LabA-like NYN domain-containing protein"
                     /protein_id="HIR3139271.1"
                     /translation="MEGRATERVAVFVDGANLYHSIKNYYKGVLDYARLLSAAVGDRK
                     LLRATFYIVEKQETEETQGTPSARSFVYNLNKFGYKVRSKPLAVHETVTASGEKTISH
                     KGDWDIGVVVDMMRLAAHADTYVLVSGDGDYVDAVEYLQSEKGIRVEVISAAQCTSQA
                     LLDVCDAHTDLADIPDLFRRSGPKQAVSDQHSAVS"
     gene            2880..3185
                     /locus_tag="AC16CM_05265"
                     /pseudo
     CDS             2880..3185
                     /locus_tag="AC16CM_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            3312..>4045
                     /locus_tag="AC16CM_05270"
     CDS             3312..>4045
                     /locus_tag="AC16CM_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3139272.1"
                     /translation="MNEIERLEVPEPLLPVGEAFREAGHELYLVGGYVRDSLLGGAHP
                     DIDATTDARPGEIKHLLRPRAEHLWTVGERFGTIGSTLGGLAVEVTTYRTDQYTRGSR
                     HPEVRFGDSLEEDLARRDFTINAMAADTLTGEVLDPFEGRQDLQSGIIRAVGDPLERM
                     RDDPLRMLRAVRFQTTLSEPERPFAMTLDLEGAISENAHWLESISAERKREEFEKILV
                     SENVASGLRTLVRLGLMPYIVPEFMET"
BASE COUNT          752 a         1308 c         1245 g          740 t
ORIGIN      
        1 ccacccctga tggtttcctg cggacgcacg tcgtagtggt ggccgactat gcagtcttcg
       61 aggtatgccc cgtcgccgac gctcgcgcca gggaggagga tgctgcgttt gatggtggcg
      121 tttggccgga tccagcagtc ggtcccgatg gttacgtctt ccagtagctt gacgccccgg
      181 ccgaccacgg cgtctcttcc cacgaggacc tggccctcta tctccgcgga agggtggatc
      241 tgggcgtcct ctccgaccca gaaggactcg ccccgcttct cgccagggat cctgacgtca
      301 accttgccgg agagcacgtc gtactgggcc tggcggtagg cctcgagcgt tcctatgtcc
      361 gaccagtaga agtcctcctc gtagccgacg aatttctccc cgttctcgag cagcttcgga
      421 aagacgttct cggcgaagtc gaagaaggcc ccctccggga tgtagtccag agcccgtggc
      481 tcgaacacgt agatgcccgt gttggccagc gtgcttatgg cctcccccgg gtctggtttc
      541 tcctggaaac cctcgatgtt accctcgtcg tccacgtcca ctaccccgaa ctccgaggtg
      601 tcgaaaaccc ggcgcaacgc gatggtggcg agggcgcccc gctctttgtg gcgctccacc
      661 agctccgtga tgttcacgtc ggtcagcgcg tcgcccgaga tgacgacgaa ggtctcgtcg
      721 aagcggtcgg cgagacgctt cacgcccccg gccgtgccga gcagcttgtc ctcccgctcc
      781 aggtagaccc tcatgccgtc caccttggac tcctcgccgt aggccccgag gagggcgtcc
      841 gccaggtagt gtacgttgac gcgaacctcc tcgatgccgt ggcgggcaag cagctcgaag
      901 atgtgctcga ttatcggagt gttcaccacg ggggccatcg gtttgggtat ctcccccgtt
      961 agcgggaaga gcctggttcc cttgcccgcc gcaagcgcca tcgccttcat aagcctcttc
     1021 ctctctgcaa agctttactc agtactctat attccctacc gtgcacacgg gcactctaca
     1081 acggctcttc ccctcggcca agctcgacga gcccctcaag cgctatacgg cctggaagat
     1141 agggggaccc gcagacgccc tcctcgagcc agggacggtg gaggacctga tctccgccac
     1201 acagatagcc cgagagcacg acatccccgt gactatactc ggcggcggca cgaacgtgct
     1261 cgtccgcgac ggtggcatca ggggcctcac catccgtctg gccaagtcct tgaggaacgt
     1321 ggagatcgaa ggcacgaccg tcaccgcaga cgccggcgtc ctctaccccg tcctggccaa
     1381 caccaccgcc ggccgcggcc tcgccgggct acagttcgcc accggtatcc ccggtaccgt
     1441 gggcggcgcc gtctacatga acgcaggcgc ctacggctcc gaaacggccg aagtgctcga
     1501 ctgggcagac gtacttagag acggcgaagt catccgaatg gagaacagag acctcgatct
     1561 ctcctaccgt tacagcgccc tgcacgacca cccgggttgg atcgtcctgc gcgccggcta
     1621 cacgctcgtt ccgggcgacc caacggagct gaaggctcgc ataaaagagt tcagggcgca
     1681 gcgcatgagc ggctccccga acaggccaag ctgcggttcc accttcaaac gcccccaggg
     1741 agatttccca ggccgcgtga tagaagcagc cggcctgaaa ggcacgcgcg tgggaaacat
     1801 cgaggtctca cccgtacacg ccaactacct ggtcaacctc ggcggtggca ccgcagaaga
     1861 cgccatagcc ctgatcgagc tggtaagaaa aaccgtccag gagaaactag gcatcgaact
     1921 cgagtcggaa gtaagaatag tcggcgagcc gtagaaaagc ttcaggttac aggaaaatta
     1981 acctgaagcc tgtggcctgc cacctgtagc ctgacacctg tagccttccc tctcagctaa
     2041 cggctgagtg ctgatcgctg acagcctgct tcggcccgga ccggcggaac aggtcgggga
     2101 tatccgcgag gtcggtgtgg gcgtcgcaga cgtcgaggag ggcctgggag gtgcactggg
     2161 ccgcgcttat gacctcgacg cggatgcctt tctcgctctg gaggtactcg acggcgtcca
     2221 cgtagtctcc gtcgccggaa acgaggacgt aggtatcggc gtgggcggcg aggcgcatca
     2281 tgtcgacgac gacgcctata tcccagtcgc ccttgtggga gatggtcttc tcccccgagg
     2341 cggtcacggt ttcgtgtacg gcgagcggct tggagcgcac cttgtagccg aacttgttga
     2401 ggttgtagac gaaagagcgc gccgagggcg taccctgcgt ctcctcggtc tcctgcttct
     2461 cgacgatgta gaaggtcgcc ctcaggagct ttctgtcccc gacggccgcg gagagaagcc
     2521 tggcgtagtc aaggacgccc ttgtagtagt tcttgatcga gtggtagagg ttggccccgt
     2581 cgacgaacac ggcgacccgc tctgttgctc tcccttccat agcgcccgaa tattagcaga
     2641 aatcatggca tgcccgtgag gtagctgtca gcactcagct agtagctatc agctttcagc
     2701 cctcagctat cagcaaggaa aagagctgat gaccgaacgg tcggtccgca ggggccaaaa
     2761 tccagcgggc cgcactagtt taccgggcca gctacttgcg ataatatccc aggggctgat
     2821 gggcatgaat tagagagggg tgaaccggca cggaattcga ggtcaacaag gtcgagcacg
     2881 ccgaagggta ctccatcatc tccgtgcgcg gagagatgga cctccatacc gcccccacgt
     2941 tccagcatgc catagagcgt gccgccaacg acgtggatac ggtcgtcgtc gacatgggcg
     3001 ccgtggcgtt catggactcc accgccctct cctccctcat gcgttccaaa gacaccctcg
     3061 accagaaggg cgtctccctg cgcctcgccg ccccctcgtc ggccgtcgaa cgcatcttct
     3121 ccgtcaccgg cttttctgac tacttcgaga tcttcccctc ccgggaggct gctatccccg
     3181 cctgaaaggc gaaaagcttc tagctgtcag ccatcagctg tcagcttctg gttcttctgg
     3241 ggtgttgcgt ctggtgctgg ctgggccgat gtgatgctgg ggctctagcg tactagaatc
     3301 agatggtggt tatgaacgag atagagaggc tggaggttcc ggagccactg ttgcccgtgg
     3361 gcgaggcgtt ccgggaggcc gggcacgagc tctacctggt cggcgggtac gtgcgggaca
     3421 gtttgctcgg cggggcacac cccgacatcg acgcgacgac ggatgccagg ccaggggaga
     3481 tcaaacacct cctcaggccg cgcgccgagc acctgtggac cgtgggggag cgtttcggga
     3541 ccatcgggtc taccctgggc ggccttgccg tcgaggtaac gacctacagg acagaccagt
     3601 acacccgtgg cagccgtcac cctgaggtgc gctttgggga tagcctggag gaggacctcg
     3661 ccagaaggga cttcaccatc aacgccatgg ccgccgacac gctcacgggg gaggtcctcg
     3721 accccttcga gggcaggcag gacctgcagt ctggcatcat ccgcgccgtc ggagaccccc
     3781 tggagaggat gcgggacgac cccctgagga tgctccgggc ggtccgtttc cagaccaccc
     3841 tgagcgaacc cgagaggccc ttcgcgatga cgctggacct cgaaggggcc atcagtgaga
     3901 acgcccactg gctggagagc atctccgccg agcgcaagcg ggaggagttc gagaagatcc
     3961 tcgtctccga gaacgtggcc tccggcctga ggacgctcgt gcgcctcggg ctaatgccct
     4021 acatcgtgcc cgagttcatg gagac
//
LOCUS       DBNGVD010000190         5490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4047352, whole genome shotgun sequence.
ACCESSION   DBNGVD010000190 DBNGVD010000000
VERSION     DBNGVD010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4047352"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /gene="acnA"
                     /locus_tag="AC16CM_05275"
     CDS             complement(<1..2036)
                     /gene="acnA"
                     /locus_tag="AC16CM_05275"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3139273.1"
                     /translation="MANDPFDARKTLTTDSGDVEIYSLKTLDEKTEGDVFSLPFSIRV
                     MLESLLRNAGGKFVSERDVEDLASWPESVGEELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMQSVGGDPGKINPLVPTDLVIDHSVQVDAFGTPAALQFNAEREFQRNRERYEFLKW
                     GQQAFDNFSVVPPATGIIHQVNLEYLAKGVIVKDGVAMPDTLVGTDSHTTMINGLGVL
                     GWGVGGIEAEAVTLGQPYYMLVPEVIGFKLTGALREGVTATDLVLTVTQMLRSHGVVG
                     KFVEFYGEGLSKLSLPDRATIANMSPEFGATCTFFPVDAETLGYLRGTGRDEELVGLV
                     EAYSREQGLWRTDETPEPRFSEMLELDLTSVEASLAGPRRPQDRVALDDMQPSFRQAL
                     AEMVDPDHPLLGDGDDAYNHADEESFPASDTPNPDIRTGGAASTGESHTTDDTESFPA
                     SDAPAGASDATNEGGGDPTSGAEPGNGKAEVEPAGSITVTVDGEELSLGHGSAVIAAI
                     TSCTNTSNPSVMMGAGLLAKKAVERGLTVAPHVKTSLAPGSKVVTEYLQTSGLLDPLE
                     RLRFDVVGYGCTTCIGNSGPLAEEISTAVEENDLVVAAVLSGNRNFEGRINPDVRANY
                     LASPPLVVAYALAGTVDIDFSRDPIGEDGSGDPVYLSDIWPSQEEVAREIDNAL"
     gene            complement(2108..3670)
                     /locus_tag="AC16CM_05280"
     CDS             complement(2108..3670)
                     /locus_tag="AC16CM_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR3139274.1"
                     /translation="MLLGAALALLSGSLYAATLAPTVLPYGAPDTLDSPMLQAEVSVL
                     GIGHPTGYPTYMMLTHLFTYLPVGDPAYAINLASAVYGVAAVLVVYLAGLRLCGRPVA
                     AAAGALAFGLSDAFWSQAVISEVYTLEALMMASVILALLAWRDTHADRYLLVSALLVG
                     LSLTHHLTSVLLVPAVLAFVFLTDRGVFSKVGLGLKGLGLFLLGLLPFLYLPIRAIMH
                     APLNEADPSTPWRFLLLVTGGSFLAESSEPGRHCSPSALSLADPSTKLGLLWDQLLGQ
                     FSVILVLVGLFAAFFLLFRDRAVAVLLGTLFFGCLGQAVVYLQLGIEDFYVFLIPAFL
                     VFGLCISAGLGAALRWAEGLGGGPAVGVVVVSLLSLLMLAIPFVGVRAAYVEHDRSGD
                     FGGRKTIETVAGNVKQNATVLHHRSPLWYMVLVEERRRDLTLVDPFCTSWDRHTDVVW
                     PDDISASGAAERYGTGDTTGVEAARLAAEDGPVYLLANARSSLGSFREAGFRVVPVGK
                     GGELYRLVPGDR"
     gene            3775..4314
                     /locus_tag="AC16CM_05285"
     CDS             3775..4314
                     /locus_tag="AC16CM_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139275.1"
                     /translation="MAKRGYTRDTIRGGASEWDVSRPFSSYIRTLAVLAVHPVRFFEL
                     LPKIPDLRAPGLFLAFSGLPTAVMWFIFGGLYPALVGLVVPLPLSFVLAGLYHPGALG
                     GRYGYVVTWRVLAYPLGFYLPFAAVPLVGPLGAAFVGLVLMPVGLVKAQEVGALRALI
                     SCVTVAALLGITWYLLLSA"
     gene            complement(4324..4812)
                     /locus_tag="AC16CM_05290"
     CDS             complement(4324..4812)
                     /locus_tag="AC16CM_05290"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139276.1"
                     /translation="MHPTAGTEVREATIEDAAGIQRLARELAETVGDAPPVEEAIRER
                     LGELLETPRSRVLVVENGSGLVGAVSFWIKPDLAHGDIVVEVPMLVVAEDQRRNGVGR
                     LLMEEVRSTASEHDASLIELVATTTNVVAREFYNSLGFVEADVVSLEFVGSHENPPQP
                     GD"
     gene            4951..>5490
                     /locus_tag="AC16CM_05295"
     CDS             4951..>5490
                     /locus_tag="AC16CM_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HIR3139277.1"
                     /translation="MQVLWGIGGMVVLLAIAFGLSTNRRAINPRTVFGALAIQVAFGV
                     IVLYWEAGKLVLRAVSQGVQAVINSSREGITFLFGPVLPEQGLVFAFQVLPVIIFFAS
                     LTSVLYHWNVLQWVVRIIGGGLQRALGTSQAESMNATANIFVGQTEAPLVIRPYISNM
                     TRSEFFAVMVGGLSTVAGSV"
BASE COUNT          910 a         1792 c         1830 g          958 t
ORIGIN      
        1 tcgagggcgt tgtctatctc ccttgccacc tcctcctggg agggccatat atcgctcagg
       61 tagacgggat ccccggatcc atcctcacct atggggtccc gggagaagtc tatgtcaacc
      121 gtgccggcca gggcgtaggc aacgacgagc ggcggggagg ccaggtaatt ggcgcgcacg
      181 tcggggttga tgcggccctc gaagttcctg tttcccgaga gcacggcggc aacgaccagg
      241 tcgttctcct cgacggcggt ggagatctcc tccgccagcg gtcccgagtt gccgatgcag
      301 gtcgtgcacc cgtatccgac cacgtcgaac ctcagccgct ccaggggatc gagcagccca
      361 gaggtctgca ggtactccgt tacgaccttt gaaccgggcg cgaggctggt cttgacgtgc
      421 ggggcgacgg tgagccccct ctccacggcc ttcttcgcca gcaggccggc gcccatcatc
      481 accgaggggt tggaggtgtt tgtgcagctc gttatggctg cgatgaccgc cgagccgtgc
      541 ccgaggctta gctcctcgcc atcgaccgtc acggtgatcg aaccggccgg ctccacctcg
      601 gccttgccgt tccccggctc ggcccccgag gtcgggtctc cgccgccctc gttggttgca
      661 tcggaggccc ctgccggggc gtcgctcgcc gggaaggact cggtgtcgtc cgtggtgtgg
      721 ctctcgcccg tagacgcggc cccgccggtg cggatgtccg ggttcggggt atcgctggcg
      781 gggaaggatt cctcgtcggc gtggttgtaa gcgtcgtccc catccccaag tagtggatgg
      841 tcggggtcga ccatctcggc cagcgcctgg cggaaggatg gctgcatgtc gtcgagggcc
      901 accctgtcct gcgggcggcg cggcccggcg aggctcgcct cgacggaggt gagatcgagc
      961 tccagcatct ccgagaagcg gggctcgggc gtctcgtcgg tgcgccagag gccctgctcc
     1021 ctggagtagg cctcgaccag cccgacgagc tcctcgtcgc ggcccgtgcc gcgcaggtaa
     1081 ccgagcgtct cggcgtccac ggggaagaac gtgcaggtgg ccccgaactc gggggacatg
     1141 ttggcgatgg tggccctgtc ggggagcgag agcttgctga ggccctcccc gtagaactcc
     1201 acgaacttgc cgaccacgcc gtgcgagcgc agcatctggg tcacggtcag gacgaggtcc
     1261 gtggccgtca cgccctcacg cagggccccc gtcagcttga acccgatgac ctcggggacg
     1321 agcatgtagt agggctggcc cagggtgacg gcctccgcct cgatgccacc aacgccccat
     1381 cccaggacgc cgaggccgtt gatcatggtg gtgtggctgt ccgtgccgac cagcgtgtct
     1441 ggcatcgcga cgccgtcctt cacgatgacg cccttcgcca ggtactccag gttgacctgg
     1501 tggatgatcc cggtggccgg gggaaccacg gagaagttgt cgaaggcctg ctggccccac
     1561 ttcaggaact cgtagcgctc gcggttgcgc tggaactcgc gctcggcgtt gaactgcagg
     1621 gccgcaggcg tcccgaaggc gtccacctgc accgagtggt ctatcaccag gtcggtcggt
     1681 acgagggggt tgatcttgcc cgggtccccg cccacgctct gcatggcgct ccgcatggcc
     1741 gcgaggtcga cgaccgcggg caccccggtg aagtcctgca tgacgaccct ggccggcaag
     1801 taggccagct cctcaccgac ggattcgggc caggacgcga ggtcctccac atccctctcc
     1861 gagacgaact tgcccccggc gtttcgtagc aacgattcca gcatgaccct gatggagaag
     1921 gggagcgaga acacgtcccc ctctgtcttc tcgtccaggg ttttcaggct gtagatctct
     1981 acgtcacccg aatcggtggt cagcgtcttg cgtgcgtcga aaggatcgtt agccatgcca
     2041 gcaacctctt ctccaagttt tcgctatgtg tgatccgcac atcagattat agatgaaagc
     2101 gccgggttta ccggtccccc ggcacgagcc tgtagagctc accgcccttg ccgaccggca
     2161 ccacgcggaa gccagcttcc cggaaggacc ccaggctgct gcgggcgttg gccagaaggt
     2221 agacggggcc gtcctcggcc gccaggcgcg cggcctcgac gcccgtggtg tcgccagtac
     2281 cgtaacgctc tgctgccccc gaggcgctta tgtcatcggg ccacaccacg tccgtgtggc
     2341 ggtcccagga ggtgcagaag gggtcgacca gggtgaggtc ccgcctccgc tcctctacca
     2401 gcaccatata ccagagcggg ctcctgtggt gcagcaccgt ggcgttctgt ttcacgttgc
     2461 ctgccacggt ttcgatggtc ttccgtcccc cgaagtcccc gctgcggtcg tgctccacgt
     2521 aggctgcacg caccccgacg aagggtatgg ccagcatcag gagggagagg agagagacaa
     2581 cgaccacgcc gacggcgggg ccgcccccca gcccttcggc ccaccgcaac gcggcgccaa
     2641 gcccggccga gatgcacagc ccgaagacaa ggaaggcggg aataaggaac acgtagaagt
     2701 cctcgatgcc gagctgaagg tatacgaccg cctggcccag acaaccgaag aaaagcgtgc
     2761 cgagaagcac ggcaaccgcg cgatccctga agagcaggaa gaaggcggcg aacaggccga
     2821 ccagcaccag gatgacggag aattgtccaa ggagttgatc ccagagcaat cctagcttcg
     2881 tcgatgggtc ggctagcgag agggccgacg ggctacaatg cctgcccggc tctgaagact
     2941 ctgccagaaa gcttcccccg gtgacgagca gcaagaagcg ccacggggtc gagggatctg
     3001 cctcgttcag gggggcgtgc attatcgccc ttatggggag gtagaggaac ggaaggaggc
     3061 cgagcaggaa gagcccgagg cccttcagcc cgaggccgac cttcgaaaac acgccccggt
     3121 cggtcaggaa cacgaaggcg aggaccgctg gcacgagcag gacgctcgtc agatggtggg
     3181 tcagggagag gcccacaagg agggccgaga cgagcaggta gcggtcggcg tgggtatctc
     3241 tccaggcgag cagcgcgagg atgacggacg ccatcatgag ggcttcgagg gtgtagacct
     3301 cggagatcac ggcctgagac cagaaggcgt cggagagccc gaaagcgagc gcaccggcgg
     3361 ccgcggcgac gggccgtccg cacagcctga gccctgccag gtagacgacg agcacggccg
     3421 cgacgccgta gaccgcggag gcgaggttga tggcgtaggc cgggtccccc accggcaggt
     3481 aggtaaagag gtgcgtcagc atcatgtagg tcgggtagcc cgtcgggtgg cctatcccga
     3541 gaaccgagac ctcggcctgt agcatcgggg agtcgagcgt gtcgggggcc ccgtaaggca
     3601 ggaccgttgg ggccagggtc gcggcgtaga gagaaccgga gagcagcgcc agcgccgcac
     3661 cgagcagcac gggggtcctg cgccaagggg gcttcgttcc tcgggagcca ttcatcgcag
     3721 gggcagaata ctacgtcggt gcggttgccc accccgggtc tgtatactcg gcccgtggca
     3781 aagcgcggct acacacggga taccatccgg ggcggggcca gcgagtggga cgtatcgcgg
     3841 cccttctcca gctacatcag gacgctggcc gtgctggccg ttcacccggt caggttcttc
     3901 gagctgctgc cgaagattcc ggacctcagg gcgccggggc tctttctcgc cttctccggc
     3961 cttccgaccg cggttatgtg gttcatcttc gggggcctct accctgcgct cgtgggcctc
     4021 gtagtgcctc taccgctctc gttcgttctc gccggcctct accaccctgg cgcgctcggc
     4081 ggccggtacg gctacgtcgt gacctggagg gtcctagcct atcccctcgg cttctacctg
     4141 ccgttcgccg ccgttcccct ggtcggccct ctgggagccg ccttcgtcgg gctcgttcta
     4201 atgcccgtgg ggctcgtgaa agcgcaggaa gtcggggctc tcagggccct gatctcctgc
     4261 gtcacggtag cggcgcttct ggggataacc tggtacctcc tgctctctgc ctgaagccgg
     4321 gaatcagtct cccggctggg gagggttctc gtgggagcca acgaactcca gggagaccac
     4381 gtcggcctcg acgaagccaa gcgaattgta gaactcgcgg gcgaccacgt tcgtggttgt
     4441 tgcgacgagc tcgatcaggc tggcgtcgtg ctccgaggct gtggaacgca cctcttccat
     4501 caggagcctg cccaccccgt ttcgcctctg gtcttcggcg acgacgagca tcggcacctc
     4561 gaccacgata tcgccgtggg cgaggtcggg tttgatccag aagctaacgg ccccgaccag
     4621 cccgcttccg ttctccacca cgagaacccg ggacctgggc gtctcaagga gctccccgag
     4681 ccgttctctg atggcttctt ccacgggggg agcgtcgccg accgtctcgg ccagctctcg
     4741 ggccagcctc tggatgcctg cggcatcttc gatggtcgcc tcgcgcactt cggtcccggc
     4801 tgtggggtgc attcttctcc tcggattgct acctgagggt acatacccca agcggccctc
     4861 tgtcgaacca acgcccccgg tagcccgagg ctcctggtgc tgagagaatg ccacctcagg
     4921 gaaggttaga ggctgaaagg aggctggcgc atgcaggtgc tgtggggtat cggtggcatg
     4981 gtggtgctac tcgccatcgc gttcggactc tcgacgaacc gacgggcgat aaacccgcgc
     5041 accgtgttcg gtgcgctggc gatccaggta gccttcggtg tcatcgtgct gtactgggag
     5101 gcgggcaaac tcgtcttgcg agccgtctcg cagggcgttc aggccgtcat caactcctcc
     5161 agggaaggca tcacgttcct tttcggaccc gtactaccag aacaggggct cgtcttcgcc
     5221 ttccaggtgc tgcccgtcat aatcttcttc gcttcgctga cctcggttct ctaccactgg
     5281 aacgtacttc agtgggtggt caggatcatc ggtgggggac tccagagggc cctcggaacg
     5341 agccaggccg aatccatgaa cgccaccgca aacatcttcg tcgggcaaac agaggccccg
     5401 ctcgtcatcc ggccctacat cagcaacatg acccgttcgg agttcttcgc cgtgatggtc
     5461 ggtgggctct cgaccgtcgc gggctcggtg
//
LOCUS       DBNGVD010000191         3057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_404139, whole genome shotgun sequence.
ACCESSION   DBNGVD010000191 DBNGVD010000000
VERSION     DBNGVD010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_404139"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16CM_05300"
     CDS             complement(<1..232)
                     /locus_tag="AC16CM_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3139278.1"
                     /translation="MSEQRLDPVTLSVLASALAGIAEEMGAVLIRGSYSSNIKERRDC
                     STALFDARGLMVAQAEHMPVHLGAMPEAVAAIM"
     gene            complement(229..2205)
                     /locus_tag="AC16CM_05305"
     CDS             complement(229..2205)
                     /locus_tag="AC16CM_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3139279.1"
                     /translation="MVSKDTRLGVDVGGTFTDLVALSEGNLITAKVPSTPRDQSEGVM
                     RAIETSDVGPAAVVALAHGMTVATNALLERRGARTALVTTEGFRDVLEIARQNRPALY
                     DLTHDRPPPLVPRELRFMVAERMGPQGEISPLDEDSLQKAIAAVEAAEVEAVAVCLLF
                     AFMHPDHEKRVGEALREALPGVHVSLSSEVLPEFREYERFSTTAADAYLAPRLAAYFS
                     NLAEKAEEAGVPGPLIMQSSGGVVPVDDAISDAAGCVLSGPAGGVVGAAYVGEKGGFR
                     DLLTFDMGGTSTDVAPIIGGEAQTTTETVVAGVPIKLPMVDVHTVSAGGGSIAWADPG
                     GALRAGPQSAGAEPGPAAYDAGGEEPTVTDANLFLGYLADRAELGGEVVLRRDLSERA
                     LASLGKELGLDAEETALGILRVADAEMVRALRVISVERGLDPRDFALLAFGGAGGMHA
                     CSLAEELGIETVLVPRAGGVLSALGLAISDLRRDYVSPFLSGLGEVEEDEFGRRFAEM
                     ESTAAGDLDGPEYTRRADLRYGGQSFEITVDADSFGKLEDRFHAAHEQRYGYRMDDEK
                     VELVNLRLISTVPVEKPEMSEPQQEVDARAGQREANFDGGWKEVAVLDREKMGEGSEV
                     VGPAIVEFKESTCVVRPGWQGAVDGVGTLVLERR"
     gene            complement(2240..>3057)
                     /locus_tag="AC16CM_05310"
     CDS             complement(2240..>3057)
                     /locus_tag="AC16CM_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139280.1"
                     /translation="LGSALATVLAATLPEAAMGSWGWRIPFLLALPLGIVGLYIRLRL
                     EDTPEFRALEESGDVAGSPFMETVRENWKPILQIAGLVVIHNVGFYLVFTYMSTYFPT
                     YLGFSTTSAFVSTTLAGLVAMALIPPLGALSDRVGRKPLLVSASVLFAVLAYPLFALM
                     NTDSLTLAVSAHVVFAVIEALFVCCSLAAGAELFTTRVRSGGFGIGYNFSVAIFGGTA
                     PYIATFLIDRTGNPLSPSYYVIFAAVVTLITVLTMRETAGHRLPQTSAEAEAT"
BASE COUNT          504 a         1069 c         1004 g          480 t
ORIGIN      
        1 ccatgatggc tgccacggcc tcgggcatcg cgccgaggtg gaccggcatg tgctcggcct
       61 gggcgaccat cagcccgcgg gcatcgaaga gcgcggtgga gcaatcgcgc cgctccttta
      121 tgttcgagga ataggaaccc cgtatcagga cggcccccat ctcttccgct atgccggcaa
      181 gggcgctggc tagcacggag agggtcaccg gatccagcct ctgctcgctc atcttctctc
      241 cagcaccaac gtgccgacgc cgtccacggc cccctgccag ccgggcctga ccacgcacgt
      301 cgattctttg aactccacta tggccggccc caccacctcc gagccctcgc ccatcttctc
      361 ccggtcgagc accgccacct ccttccaccc gccgtcgaag tttgcttccc gctgtccggc
      421 ccgcgcgtcg acctcctgct gcggctcgct catttccggc ttctcgacgg ggacggtgga
      481 gatgaggcgc aggttgacca gctcgacctt ctcatcgtcc atgcggtaac cgtagcgttg
      541 ctcgtgggcg gcgtggaagc ggtcctccag cttcccaaaa gagtcggcgt ccaccgtgat
      601 ctcgaacgac tgccctccgt aacgcaggtc tgccctgcgg gtgtactccg gcccgtcgag
      661 gtcccctgcc gccgtactct ccatctccgc gaacctccgc ccgaactcgt cctcttccac
      721 ctcgccgagg ccgctcaaga acgggctgac gtaatcgcgg cggaggtcgg agatagcgag
      781 gccgagcgcg ctcaggacgc cgccggcacg ggggacgagc acggtctcta tgccgagctc
      841 ttcggccagg gagcaggcgt gcatgccgcc ggcgcccccg aaggcgagga gggcgaagtc
      901 cctcgggtcg agaccgcgtt ccacgctgat cacgcgcagc gcccgcacca tctcggcgtc
      961 ggccacccgc agtatcccaa gcgcggtctc ttccgcgtcc aggccgagct ctttcccgag
     1021 ggaggcgagg gcccgctcag agaggtcgcg cctgagcacc acctccccgc caagctcggc
     1081 cctgtcggcg agatagccga ggaacaggtt ggcgtccgtg acggtgggtt cttcgccacc
     1141 cgcgtcgtag gctgcggggc ccggctcggc ccccgccgac tgagggccgg cgcgcagggc
     1201 cccgcccggg tcggcccacg cgatagaacc cccgccggcg ctcaccgtgt gcacgtctac
     1261 catgggaagc ttgatgggca cgccggccac caccgtctcc gtggtcgtct gtgcctcccc
     1321 cccgatgata ggcgccacgt ccgtggaagt gccgcccatg tcgaaggtca gaaggtcgcg
     1381 gaaaccgccc ttctccccga cgtaggcagc gccgacgacg cccccggcgg gtcccgagag
     1441 cacgcagccc gctgcgtcgg agattgcgtc gtcgaccgga accaccccgc cggaggactg
     1501 catgatcagg ggtcccggca ccccggcctc ctcggccttc tcggccaggt tggagaagta
     1561 cgccgccagc ctcggggcca ggtaggcgtc agcggccgtc gtggagaacc gctcgtactc
     1621 cctgaactcc ggcagtacct cgctgctcaa ggagacgtgt accccgggta acgcctcacg
     1681 aagagcctcg cccacgcgct tctcgtggtc ggggtgcatg aacgcgaaga gcaggcagac
     1741 ggcgacggcc tcgacctcgg ccgcttccac ggcggcgata gccttctgca agctatcttc
     1801 gtcgaggggg gagatctcgc cttgcggacc catcctctcg gcgaccatga agcgcagttc
     1861 gcgcggtacg agcggcgggg gacggtcgtg ggtcaggtcg tagagggcgg ggcggttctg
     1921 acgggctatc tcaagcacat ccctgaagcc ctccgtggtt accagggccg tgcgggcgcc
     1981 gcgacgctcc agcagggcgt tggtcgccac ggtcatgccg tgggcgagcg cgactacggc
     2041 cgcgggcccc acgtcggaag tttcgatcgc cctcatcacg ccctccgact ggtccctggg
     2101 cgtcgatggg accttggccg tgatcaggtt tccctcggag agggctacga ggtccgtgaa
     2161 cgtaccgcca acgtccacgc cgaggcgggt gtccttgctc accatctggt ccccgcttcg
     2221 gacgccggca gacctggcat catgtcgcct ccgcttcggc cgatgtctgc gggagcctgt
     2281 gacctgccgt ctcgcgcatg gtaagcacgg tgatcagggt gaccactgct gcgaagatca
     2341 cgtagtagga gggcgagagg gggttgccgg ttcggtctat gaggaacgtg gcgatgtaag
     2401 gggcggtccc accgaatata gccaccgaga aattgtagcc gatgccgaag cccccagagc
     2461 gcaccctggt tgtgaacagc tcggccccgg ccgccagcga gcagcacacg aacagggctt
     2521 ctatgacagc gaagaccacg tgcgccgaga cggcgagcgt caggctgtcc gtgttcatga
     2581 gcgcgaacag cggataggcc agcaccgcga acagcacgct cgccgacacg agaaggggct
     2641 tgcggcccac gcggtcggag agggcgccaa gcggtgggat aagcgccatc gccacgaggc
     2701 cggccagcgt ggtcgagacg aaagcggacg tcgtgctgaa gcccaggtag gttgggaagt
     2761 acgtggacat gtaggtgaac acgaggtaga agccgacgtt gtggatcacg acgaggccgg
     2821 cgatctgcag tatgggcttc cagttttccc tcacggtctc catgaagggc gaccccgcga
     2881 cgtcgccgct ctcctccaac gcccgaaact ccggcgtgtc ttccagcctc agcctgatgt
     2941 acagcccgac gatgcctagc gggagcgcca ggaggaaagg tatgcgccag ccccacgagc
     3001 ccatggctgc ctcggggagc gtggccgcaa ggaccgtggc cagcgccgag cccaaaa
//
LOCUS       DBNGVD010000192         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3683560, whole genome shotgun sequence.
ACCESSION   DBNGVD010000192 DBNGVD010000000
VERSION     DBNGVD010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3683560"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="sufB"
                     /locus_tag="AC16CM_05315"
     CDS             <1..1071
                     /gene="sufB"
                     /locus_tag="AC16CM_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3139281.1"
                     /translation="QAERDSLAGVGAQYESEVVYHSLKEEWENSGVVFMDMDGGLREH
                     EDIVKEYFGTIIPPDDNKFAALNSAVWSGGSFVYVPPGVHIDIPLQAYFRINAENMGQ
                     FERTLIIADEGSYVHYIEGCTAPTYTTNSLHSAVVELIAKPGARIRYSTIQNWSHNTY
                     NLVTKRGAAYENSTIEWVDGNLGSKLTMKYPAVYLLGEGARGEILSVAYAGDGQHQDA
                     GGKVVHAAPNTSSLITSKSISRGTGRSTYRGLLKVHEGAVNSKSRVECDALLLDENAR
                     TDTYPYIEIEEKKVNTEHEATVSKVGEDQLFYLMSRGLSEEEAMAMVVNGFIEPIAKE
                     LPLEYAIELNRLIQLEMEGSVG"
     gene            1213..2574
                     /gene="sufD"
                     /locus_tag="AC16CM_05320"
     CDS             1213..2574
                     /gene="sufD"
                     /locus_tag="AC16CM_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3139282.1"
                     /translation="MQQKEAPEILNSRGISRETVEALSSFKGEPAWLAEKRLEGWRSF
                     EALPMPTLRDEAWRYTDISDVTLEDFLPYAPSRDVASESDLPDAVQRLISEGEQNSAL
                     LVQHNSETAYSRVDEELSSKGVVFTDLHTALVEHEDIVRDKLFGLVPADYDKFAALSA
                     AAFAGGSFLYVPRGVDVELPIQSYRWLDVVGSIMPRTLVVVEEGANVTYIDEYASEAG
                     EDPAFSNGAVELYVGQGANLRYVSLQNWERNVLHFNTIRSQTEKDATINSLVVSFGSQ
                     LSRTNVEAGLSAAGSDSEMLGLYFADQNQVLDHHTLQDHLAPNAHSDLVYKGALRDES
                     LAVFSGLIRVEPGAQKTDAYQTNRNLILGTDDAFAVSLPRLEILADDVKCSHGSTTGQ
                     VDETELFYLMSRGIPRREAEKLVVFGFFGEVTSRIPLKGLKDKLDRAIEGKIGLGFEE
                     RVA"
     gene            2575..2928
                     /locus_tag="AC16CM_05325"
     CDS             2575..2928
                     /locus_tag="AC16CM_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR3139283.1"
                     /translation="MPEFHKLASADEVGPGKIKQYIIEDRFVALCNVGGEFHAFEDVC
                     THQFAHLTEGEFDDSTVTCPLHGAKFDVRTGAAKSLPAVKPVPKHEVKVENGSVYVAL
                     NPKQVKVNRRRGLRR"
     gene            2935..3429
                     /locus_tag="AC16CM_05330"
     CDS             2935..3429
                     /locus_tag="AC16CM_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3139284.1"
                     /translation="MLLEGSCHCGAVHFRVESETPYPYQACYCSICRKTAGGGGYAIN
                     LGADANTLEVEGGENLSVYNAKLYDEGGPDEVPVSDAERNFCRFCGSCLWLYAPDYPD
                     LVHPFASAIDTPLPKPPEHVRLMLDHAAPWCEIPTGEGEAHFSEYPEESLVGWHRRHG
                     LLAP"
BASE COUNT          768 a         1236 c         1246 g          666 t
ORIGIN      
        1 caggccgagc gcgactcgct cgccggcgtc ggagcgcagt acgagtccga ggtcgtctac
       61 cattcactga aagaggagtg ggagaatagc ggcgtcgtct tcatggacat ggacggtggc
      121 ctgcgcgagc acgaggatat cgtcaaagag tacttcggga cgatcattcc tcccgacgac
      181 aacaagttcg cggcgctcaa cagcgcggtc tggtccggcg gctcgttcgt gtacgtaccg
      241 cccggggtcc atatcgacat cccgctgcag gcctacttcc gcatcaacgc ggagaacatg
      301 ggccagttcg agcggacgct catcatcgcc gacgagggtt cgtacgttca ttacatcgag
      361 ggctgcaccg ccccgacgta cacgacgaac tccttgcact cggccgtggt ggagctcatc
      421 gccaagcccg gggccaggat caggtactcc accatccaga actggtcgca caacacctac
      481 aacctcgtaa ccaagcgggg tgcggcctac gagaactcga ccatcgagtg ggtggacggc
      541 aacctgggct ccaagctcac catgaagtac ccggccgttt acctcctggg cgagggtgcc
      601 aggggtgaga tcctgtctgt cgcctacgcc ggtgacgggc agcatcagga cgcaggcggc
      661 aaggtcgtgc atgcggcgcc caacacgtcc agcctcatca cctccaagtc catctccagg
      721 gggactggta ggagcacgta tcgcgggctc ctcaaggtgc acgagggcgc ggtcaactcc
      781 aagagcaggg tcgagtgcga cgcgttgctg ctcgacgaga acgccaggac ggacacctac
      841 ccgtacatag agatcgagga gaagaaggtc aacaccgagc acgaggccac cgtctccaag
      901 gtcggtgagg accagctctt ctacctcatg agccgcgggc tctccgaaga ggaggccatg
      961 gccatggtcg tcaacggctt catcgagccc atcgccaagg agcttccgct cgagtacgcc
     1021 atcgagctga accgcctgat ccagctcgag atggaaggct cggtcgggta gagagctatc
     1081 agctgtcagc cttcagcggt gggctttctg gctcgggtgg cgttgaccgg accgtagagt
     1141 aacgggtgat ctatggaggg cgggccctgt atttgggacc cgccctcgtt ttgaggagta
     1201 aaggcaggtc ttatgcagca gaaggaagcg ccggagattc taaacagcag gggtataagc
     1261 cgggagacgg tggaggccct ctcctccttc aagggtgagc ccgcatggct cgcggagaag
     1321 cgcctggagg ggtggcgtag cttcgaggct ctcccgatgc ccaccctgcg cgatgaggcg
     1381 tggcgttaca cggacatctc cgacgtcacc ctggaggact tcctacctta cgccccgagc
     1441 cgggacgtcg cgagcgagtc ggacctgcct gatgcggtac agcgcctcat cagcgaaggc
     1501 gagcagaact cggccctgct cgtgcagcac aactcggaga cggcgtacag ccgggtggac
     1561 gaggagctct cgagcaaggg cgtcgtcttt acggatctgc acacggcgct cgtggagcac
     1621 gaggacatcg tcagggacaa gctcttcggg ctcgtgcccg ccgattacga caagttcgcg
     1681 gccctgagcg cggcggcttt cgccggcggc tccttccttt acgtgccccg cggggtggac
     1741 gtggagttgc cgatccagag ctatcgttgg ctcgacgtag tgggctccat catgccccgt
     1801 actctggtcg tggtcgagga gggtgccaac gtaacctata tcgacgagta cgccagcgaa
     1861 gccggggagg accccgcgtt ctccaacggg gccgtcgagc tctacgtagg gcagggcgcc
     1921 aatttgcgct acgtctccct gcagaactgg gagcggaacg tgctccactt caacaccatc
     1981 cgctcacaga ccgagaagga cgccacgatc aacagcctcg tggtctcctt cggctcgcag
     2041 ctctcgcgca ccaacgtcga ggccggactg tcggctgctg gcagcgattc ggagatgcta
     2101 ggtctttact tcgcggacca gaaccaggtt ctcgaccatc acacgctgca ggaccatctc
     2161 gccccgaacg cccactcaga cctcgtgtac aagggcgctt tgcgcgacga gtccctcgcc
     2221 gtcttcagcg ggctcatccg cgtcgagccg ggggcgcaga agaccgacgc ttaccagacc
     2281 aaccggaacc tcattctagg gaccgacgac gctttcgcgg tgagccttcc acgcctggag
     2341 atactggccg acgacgtgaa gtgctcgcac ggttcgacga cggggcaggt ggacgagacg
     2401 gagttgttct atctcatgag ccggggcatc ccgcgccgcg aggccgagaa gctcgtcgtg
     2461 ttcgggttct tcggggaggt aacgagtagg atccccctga agggtttgaa ggacaagcta
     2521 gaccgggcca tagagggcaa gatcgggctc ggcttcgagg agcgtgtcgc ctaggtgccc
     2581 gagtttcaca agctggcatc cgcggacgag gtgggtccgg ggaagatcaa gcagtacatc
     2641 atcgaggacc gcttcgtcgc cctgtgcaac gtggggggcg agttccacgc cttcgaggat
     2701 gtctgcaccc accagttcgc ccacctgacc gagggtgagt tcgacgactc cacggtgacg
     2761 tgccccctcc acggcgcgaa gttcgacgtg aggacgggcg ccgcgaagag cctgcccgcc
     2821 gtgaagcccg tcccgaagca cgaggtcaag gtggagaacg gcagcgtcta cgtcgcgctc
     2881 aacccgaagc aggtcaaggt caaccgcagg agaggcctca gaaggtaacc cgtcttgctc
     2941 ctcgaaggtt catgccactg cggggcggtc cacttcaggg tcgagtccga gacgccctac
     3001 ccgtatcagg cctgctactg ctccatctgc cgcaagaccg ccggtggagg cggctacgcc
     3061 atcaacctgg gggccgacgc gaacaccctg gaagtagaag gcggggagaa cctctccgtc
     3121 tacaatgcga agctctacga tgagggcggg ccggatgagg tgcccgtcag cgatgcggag
     3181 cggaacttct gccgcttctg cggaagctgc ctctggctct acgcaccgga ctaccctgac
     3241 ctcgtccacc ccttcgcttc ggcgatagac acgccgctac cgaaaccgcc cgagcacgtt
     3301 cgcctcatgc tcgaccacgc cgcgccctgg tgcgagatcc ccaccggaga aggcgaagca
     3361 catttctccg agtaccctga ggaatccctc gtggggtggc accgccgcca cgggcttctg
     3421 gctccctaga gcagtttgcg gaaaagcttg tgggagccct tgtcctgggg gcctattcat
     3481 cggaagacgt agaagaccgc ctggccaagc ttcgcccaaa cggcgttctt agaagttcgc
     3541 cacacgctcg caacgcccaa gctacggcaa agatagttac tttaggggat gctctggccg
     3601 caataggagc gtatgctgcc ttcgttacgt ggccagctga ggctgcgtga gggcgcaggt
     3661 tcgctacctg ccctccttct agcccggggc tagccaccca ccccactacc agcccaccag
     3721 gcacgaaagg ggaccccctc acaacgggta ggccgggctg ctcggccgct caggaacgag
     3781 agccaccgcc catccaaacg ctcgaagaga tgataacgag gagatagtgt gtcgagaaaa
     3841 gtgatcttgc tggcagcgat cgtggccctg gtgttggctg ccgccgtccc ggcgctcgct
     3901 agtagccgga gcccca
//
LOCUS       DBNGVD010000193         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3684194, whole genome shotgun sequence.
ACCESSION   DBNGVD010000193 DBNGVD010000000
VERSION     DBNGVD010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3684194"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="AC16CM_05335"
     CDS             <1..1889
                     /locus_tag="AC16CM_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3139285.1"
                     /translation="TMLGLGLGIDYALFFVSRFREELESFPTSEAVPRTVATAGRSIF
                     FSGTAVLIGLSGLLFFPFMFMRSIGVAGVIVVFVSVIAALTLLPALLAVLGPNVNRLA
                     IRRRGNGMGTAFWSRSAEVVMRRPFAVILLTALLLGVLLYPVGHMKVGIPEATVLPQK
                     YESRAGDDILKENFEYAELNPMEIVATLNEDPLSAAGLADTKELGEKVSGSEGVARVE
                     SVYAIGKAAARDYTVRVEDARAEAQRQAAGKVDGLVEAQIEELIQKNTDEAVEGQLAE
                     LEATYGTVPPGAEDQVRAEVQPRVEEEVRASEERIREEVERRVAAEVDRKVPDLPEGI
                     SSDGMVTPEGVANFLKLPAARDSAELQNALDSYVANDRTLLRGVTQANPYTQDAYAAV
                     DAVRAVDPPTGTNFLVGGLSAGQEDFISSLYGKAPYAAAFVIGVTYIVLLITFRSVIL
                     PLKAVIVNILSLTASFGAMVFVFQDGNFSGLLNFTPLGFVDATLPILMFCTIFGVSMD
                     YEVFLLSRIREAYENGDSNTASVSKGLVATAGIITSAAAIIMVVTGAFAFTGIVLTKA
                     IGLGLAVAVFVDATIIRILLVPATMRILGDWNWWPGGRKTTFGADPKKSSLPDDDRQL
                     KAEG"
     gene            1945..2355
                     /locus_tag="AC16CM_05340"
     CDS             1945..2355
                     /locus_tag="AC16CM_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3139286.1"
                     /translation="MVEFERILKDRGYRLTNQRRIIVRELEGERHLSAEEIYDRVKGD
                     HPELGLSTVYRTLDLLTELGIVRKDDFGEGYARYELATERVHHHARCGECGAVIEFNE
                     ELMEYLALQVERETGFVTSWHEITLHGRCANCAR"
     gene            complement(2444..2929)
                     /locus_tag="AC16CM_05345"
     CDS             complement(2444..2929)
                     /locus_tag="AC16CM_05345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139287.1"
                     /translation="MQPLVRPYEEQDTAALTELSLRAWAPVFESLEQTLGPAIFRRQH
                     PDWREDQRQAVERLCASEETRIWVAEVDGEIVGFVGTGVFDPELAMGEISILAVDPDH
                     QGSGIGTALTEFALDRLAEAGMRVAMVETGGDPGHAPARRTYEGAGFTLLPVARYFKN
                     L"
     gene            complement(2967..3725)
                     /locus_tag="AC16CM_05350"
     CDS             complement(2967..3725)
                     /locus_tag="AC16CM_05350"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3139288.1"
                     /translation="MRITVIGTGTVVPSLQRRQSCVVVRTGGETLVFDLGSGAVRGML
                     RAGLDPFAVDRIFFTHFHPDHTVDAVPLLFAINYGSQEDRTRPLHVSGPQPFAEFWAS
                     LMKVWGRWMTGDYATLLSELPRECSSPIELAGCRLAWSPAEHRHESIAYRLDTAGGGF
                     VYTGDTEYSESVVELARGADTLLIECAFPDHAPVPGHLTPSGVASMASEAGVRRVVLT
                     HIFPATDLLDLVAEVGKGFDGEIVVAEDGLTLEV"
     gene            complement(3876..>4001)
                     /locus_tag="AC16CM_05355"
     CDS             complement(3876..>4001)
                     /locus_tag="AC16CM_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3139289.1"
                     /translation="AASRLYLGTHWPSDMLGGALLGIAGLAWAFGSHQPSAISRQ"
BASE COUNT          726 a         1251 c         1271 g          753 t
ORIGIN      
        1 ctaccatgct tggcctcgga cttggcatcg actacgcgct gttcttcgtg agccgtttca
       61 gggaagagct ggagagtttc cccacttcgg aggccgtgcc gcgcacggtg gccacggcgg
      121 ggaggtcaat cttcttctcg gggacggcgg tgctgatcgg tctctccggg ttgctgtttt
      181 tccccttcat gttcatgcgc tctatcgggg tagccggggt gatcgtcgtg ttcgtctcgg
      241 ttattgccgc cctgacgctc ttgcccgccc ttcttgcggt gctcggaccg aacgtgaaca
      301 ggctggcgat ccggcgccgc ggaaacggga tgggcacggc cttctggagc cgcagcgcgg
      361 aggtggtgat gcgccgcccg ttcgccgtga tcctgttgac cgccctcctc cttggcgtct
      421 tgctctaccc cgtagggcac atgaaggtcg gaattcccga ggcgaccgtc ctgccccaga
      481 agtacgagtc ccgcgcaggt gacgacatcc tgaaagagaa cttcgagtac gcggagctga
      541 acccgatgga gatcgtcgcc accctgaacg aagacccgct tagcgccgcg ggtctcgcgg
      601 acacgaagga gctcggcgag aaggtgtcgg gatcagaagg cgtcgcccgc gtcgagagcg
      661 tatacgcgat cgggaaggcc gcggcccgag attacaccgt ccgcgtcgag gatgcccgcg
      721 ctgaggcgca gaggcaggcc gccggcaagg tggacgggct cgtggaagct cagatcgaag
      781 aactcatcca gaagaacacg gacgaagccg tggaagggca attggcggag ctcgaggcca
      841 cctacggtac ggtcccgcca ggggcggagg accaggtcag ggcggaagtc caaccacggg
      901 tggaggagga ggtgcgtgcc tcagaagaac gcatcaggga ggaggtagag cgtcgtgtgg
      961 ccgcggaggt ggaccggaag gtgcctgacc tgccggaggg aatctcatcg gacggcatgg
     1021 ttacacccga gggtgtcgcg aacttcctga agctgccggc ggcccgggat tcggcagagc
     1081 tccagaacgc cctggattcc tacgtggcga acgatcgaac cctgctccgg ggtgttaccc
     1141 aggcaaaccc gtatacgcaa gacgcctacg ccgcggttga tgccgttcgc gccgttgatc
     1201 ccccgacggg cacgaacttc ctggtgggtg ggctctccgc gggacaggaa gacttcatct
     1261 cgagcctcta cggcaaggct ccttacgccg cggccttcgt gatcggggtg acctacatag
     1321 tccttctcat taccttccgc tccgttattc tccccctgaa ggccgtgatc gtgaacatcc
     1381 tgagccttac ggcgagcttc ggggccatgg tcttcgtgtt ccaggatggt aacttctccg
     1441 gtctcctcaa cttcacaccc cttggcttcg tggacgcgac gctccctatc ctgatgttct
     1501 gcaccatctt cggggtctcg atggactacg aggtattcct cctctcgcga ataagggagg
     1561 cctatgagaa cggtgactca aacaccgcga gcgtctccaa gggcctcgtg gccaccgccg
     1621 gcatcatcac gtcggccgcc gcgatcatta tggtcgtgac gggagccttc gccttcacag
     1681 gtatagtcct gaccaaggct atcgggctcg gcctggctgt agccgtcttc gtggacgcaa
     1741 ccatcatccg catactcctc gtccccgcca ccatgcgcat cctgggcgac tggaactggt
     1801 ggccaggggg ccgtaagacc accttcggcg ccgatccgaa gaaatcttcc ctaccagacg
     1861 acgaccggca gctgaaagcg gagggctgac agctacataa gatcaattct tgatactggt
     1921 gcagggtaag actaaactat cagcatggtc gaattcgagc ggatactcaa ggacaggggt
     1981 tacaggctaa ccaaccagcg tcgcatcatc gtgcgcgagc tggaagggga gcgtcatctt
     2041 tcggccgagg agatctacga ccgggtgaaa ggcgatcatc cggagcttgg cctttccacc
     2101 gtgtaccgga cgcttgacct gctcaccgag ctgggcatcg tgcgcaagga cgatttcggt
     2161 gagggctacg cccgctacga gctcgcgacc gagcgtgtac accatcacgc ccgatgcggc
     2221 gagtgcggcg ccgtcatcga attcaacgag gagctgatgg agtatctggc cctgcaggtc
     2281 gagcgggaga ccggcttcgt cacgagctgg catgagatca cgctgcacgg acgctgtgcg
     2341 aattgcgccc gttgatggaa gcgggatggg gcagcacgat caccaagacg gctcatgcta
     2401 cctgctgggg gtgggatgaa gtgcttctgc gtggccggca gagttagagg ttcttgaagt
     2461 acctggctac cggcagcagg gtgaagccgg ctccctcgta ggtgcgccgc gccggggcgt
     2521 gaccagggtc gccgcccgtc tcgaccatgg ctaccctcat cccggcctcc gcgagcctgt
     2581 ccagcgcaaa ctccgtcagg gccgttccta ttcctgatcc ctggtggtca gggtcgacgg
     2641 ccagaataga gatctcgccc atcgcgagct cgggatcgaa gactccggtg cccacgaaac
     2701 ccacgatctc cccgtcgacc tccgcgaccc agatccgcgt ctcctccgag gcacacaatc
     2761 tctccaccgc ctgccgctga tcctccctcc agtctggatg ctggcgcctg aagatggccg
     2821 gtcccagcgt ctgttcgagg gattcgaata ctggtgccca ggcccggagc gagagctcgg
     2881 taagcgctgc ggtgtcctgt tcctcatacg gccgaacgag tggttgcacg atctaggtac
     2941 ctcctggaag cgcttggaac gaggcctcaa acctccaatg tgaggccgtc ttcggcaacg
     3001 actatctccc cgtcgaagcc tttgccgacc tccgccacga ggtccagcag gtcggtcgcc
     3061 gggaagatat gcgtgaggac gacgcgtcgg acgcccgctt cgctcgccat gctggccaca
     3121 cccgaaggcg taaggtgacc gggcacgggg gcgtggtcag ggaaggcgca ttcgatcagc
     3181 agcgtgtccg cgccccgggc taactcgacc accgactcgc tgtactcggt atcacccgtg
     3241 tacacaaacc cgccgcccgc cgtatcgagc ctgtaggcaa tgctctcgtg gcggtgttcg
     3301 gccggggacc aggcgagacg gcagccggcc agctcgatgg gggaggagca ctcgcgggga
     3361 agctcggaga gcagcgtggc gtagtcaccg gtcatccacc ttccccacac cttcatcaag
     3421 gatgcccaga attcggcaaa gggttgtgga cccgacacgt gcagcggtct cgtgcggtcc
     3481 tcctgtgagc cgtagttgat cgcgaagagg agcgggacgg cgtcgacggt atgatccgga
     3541 tgaaagtgcg tgaagaagat gcgatccacg gcgaaggggt ccaggccggc ccggagcatc
     3601 ccgcgtaccg cccccgagcc aagatcaaag acaagcgtct cgccgcctgt acgtaccacg
     3661 acgcagctct gtctccgctg taggctcggg actaccgtgc cggttcctat gaccgttatc
     3721 cgcatagtcc tcccttcgtc ggaagctttc agctatcagc cgtcagcttt cagctccttg
     3781 cttgcgggac aaactgagtt tcgcaccctt agggttcgtg gtccggtgtg cgtttgcgta
     3841 tcgttgtctc ctcaggtcgg gcgcccttta ttcccctact gacggctgat agctgatggc
     3901 tgatggcttc caaacgccca cgccagtccg gctattccga gaagtgcgcc gcccagcatg
     3961 tcggagggcc agtgggttcc caggtagagc ctgctcgccg c
//
LOCUS       DBNGVD010000194        16189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4048489, whole genome shotgun sequence.
ACCESSION   DBNGVD010000194 DBNGVD010000000
VERSION     DBNGVD010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4048489"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16CM_05360"
     CDS             complement(<1..484)
                     /locus_tag="AC16CM_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139290.1"
                     /translation="MHASRPEAVEEHTHTHVPLLVVGAGPYGLATAACARHAGIEQIV
                     VGEPMAFWRENMPAGMFLRSGSDWHLDAAEVNTFEAYLEERGIDPADFDPIPVGLFLD
                     YVDWFEEREDLQVVPDLVRDLSRPNGHFEATLESGGRITADAVVAAPGISHFTVVPEW
                     V"
     gene            complement(592..1122)
                     /locus_tag="AC16CM_05365"
     CDS             complement(592..1122)
                     /locus_tag="AC16CM_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR3139291.1"
                     /translation="MRRELGDLTVECIRGDITSQGDVEAVVNAANARLASGGGVAGAI
                     HRAAGPGLAEEAVPLGPIEPGEAVITGAHGLPNGYVIHTLGPVYGQDRPEPVLLARCY
                     RNSLALAEDNGVASIAFPAISTGIFGYPVEEAAETALQTVREEAERLEVVRLVRFVLF
                     GERDLEAHERVLSGLR"
     gene            complement(1119..2276)
                     /gene="nagA"
                     /locus_tag="AC16CM_05370"
     CDS             complement(1119..2276)
                     /gene="nagA"
                     /locus_tag="AC16CM_05370"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3139292.1"
                     /translation="MSLVALRGQVVTDYEVWPDGTVLLEDSSVAGVSPDDSLAADAHE
                     VHYYGEALVLPGFVDLQVNGAFGIDVASESGRLSELSRALLSTGTTSYLPTVISSPES
                     IYEEALPAIEAAASGRRSGAEVLGVHLEGPFINLSKRGAHAAAHVVRPDTELLARLLD
                     LGPVRMVTLAPELEGATALMDLASSRGAVVCAGHSDVAFEPAYGALDGSAAGVTHLFN
                     AMSSMHHREPGLPGAAFAHPRAVCGLIPDGLHVHPEMVGLAFRMLGPDRICLVTDAIA
                     AAGMEEGEYRLATRTVYVDGGVPKLGSGAIAGSVLTMRGAFKNILAFTGCTVPEAARM
                     TSTAPARLIGEGRRKGRLVSGYDADVTVLAPDLSVQAVWRGGGLAYSREGM"
     gene            complement(2351..2872)
                     /locus_tag="AC16CM_05375"
     CDS             complement(2351..2872)
                     /locus_tag="AC16CM_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139293.1"
                     /translation="MARELQGDPGASRYARRMDSESSVSYMGCLFSVLADAEQTDGRF
                     GLIEMVVPRGREPSRHLHHSDDEGFYVLDGRVIFYVGEETYLADPGTFVFLPHGVPHS
                     YAFETDVVRMLSFIAPGGLEAHFQDSRFSEPARDLALPPPGELDQDLLEEMAGDLAAY
                     GTEVVGPPGPPRS"
     gene            3106..3741
                     /locus_tag="AC16CM_05380"
     CDS             3106..3741
                     /locus_tag="AC16CM_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139294.1"
                     /translation="MIRAVLRVMLAVCLFSMLAACSEDPAGQRNGGQGSPVPSGTSAS
                     VASSGTNTSPTTETAEPAEGPVVESTVGGAVLRRDESGLKIVQARLLRAKENDEIFVK
                     ARVERPAEARDCYLMEASTWFDLRDAIESENISDVPDVLEVLWADPSSTTRFSGGDTL
                     AISFPENTDRKAGGPDTEHTSFFVVCYKTANPESNVTWYDVAHVEGTPEVP"
     gene            complement(3797..4618)
                     /locus_tag="AC16CM_05385"
     CDS             complement(3797..4618)
                     /locus_tag="AC16CM_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3139295.1"
                     /translation="MIGVLTGMPPVLQAFLATLFTWGMTAAGAALVFGTKNVPRKLLD
                     AMLGFAGGVMIAASFWSLLAPAIELAEAGSLPVWVPPVIGFLAGGVFLRLLDRLLPHL
                     HPGLKLSEAEGIHTSWRRSVLLVSAITLHNIPEGLAVGVTFGAVGIGVTEGVPGATLG
                     AAIALAIGIGLQNFPEGTAVSMPLRREGMSRGRSFFYGQLSGVVEPIAAVIGASAVLI
                     AQPILPYALAFAAGAMIFVVAEELIPEAKRGSPDIAALSLMVGFAVMMTLDVALG"
     gene            complement(4693..5985)
                     /locus_tag="AC16CM_05390"
     CDS             complement(4693..5985)
                     /locus_tag="AC16CM_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3139296.1"
                     /translation="MRGVEGVLPGETATARAAHESLRGKRRGIRKILPFLGPAFIAAV
                     AYVDPGNFATNIAGGAQFGYLLLWVILVSNLMAMLIQSMSAKLGIATGKNLPEVCRAR
                     FPKPVTFLLWVQAEIIAMATDLAEFIGAALALNLLFGIPLFPAGLLTAVGAFGILELQ
                     RRGFRPLEAVIAAMVGVIVVAFAFQMFYAEPEADRILAGLFTPGFAGTESVLLAAGIL
                     GATVMPHVIYLHSALTQRRIVGKTEQEKRRIFRFELVDVIIAMAIAGAINASMLIMAA
                     ALFHSAGLTGVGDIDKAFEQLRILEGSIPAILFGIALLASGLSSSSVGTLSGQVVMQG
                     FVNRQIPLFLRRLITMAPALIILGIGLNPSRSLVLSQVVLSFGIPFALVPLLIFCRNR
                     DVMGTLVNYKVTTALASVVVALIVSLNVFLLYKTFLGG"
     gene            complement(6135..7313)
                     /locus_tag="AC16CM_05395"
     CDS             complement(6135..7313)
                     /locus_tag="AC16CM_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3139297.1"
                     /translation="MGREKRGPAWVLLGLLPLLGLGLLLASIFIYGVGVGRSDAPPVE
                     DLTVGRVTLPTENEFVVHVQNGGPNPVTLEQVVVNDALWDFRVEPDGTIPRLGSAEVV
                     IPYMWVEGEAYEIVLISETGTTFDAEVPVAVLTPGFSGETFSEYALIGFYVGVVPVSL
                     GLLWYPFLRRLGSAGMNFVLALTVGLLVFLALDTLLEAVEIAGGLPGAFSGVPLVILV
                     ALLTLFGLLGVERLFRRGEVGRLATSYRIALGIGLHNLGEGLAIGAAFALGEAALGTF
                     LILGFTLHNITEGVGIAAPVLKEKRPSLAHFGALALLGGGPAIVGTWIGGFAYSNLFS
                     TVFLAIGVGAILQVVYEVGRLLMRDSAHSKTPVFSATNLGGLTTGIAIMYVTALLVSV
                     "
     gene            complement(7317..8744)
                     /locus_tag="AC16CM_05400"
     CDS             complement(7317..8744)
                     /locus_tag="AC16CM_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3139298.1"
                     /translation="MAAALTLGAAMIHVWAMPEHFGEWWLYGILFLLVAALQGFYGVA
                     LWRWAVKTVFILGMGGNLAIVAFYLVTRTVGVPFGPHVGEVEAVGVLGLTATICEVAL
                     VVTLAVLYRMGAEVPVRGAEVAFLEGGGKAGALSRRDFLRVTGAVGAFGASGAVLGAH
                     VERALGQEAHHQGGHVSGVGHNFHGGNGVVGDVNLSRFDPTRFLRDFYRGEERKEGGR
                     TVREYELTAVETEIEVAPGIVYPAWAYNGQVPGPTLRATEGERVRIRFKNNSVHPHTI
                     HFHGFHPANMDGVFELVGTGQEFVYEFDAEPAGLHLYHCHTAPLRKHIEKGLYGAFII
                     DPKEGRPEADEMVMVMNGFDTNFDASNEVYAVNTVAFHYQRHPIQIVKDRLIRVYAVN
                     VLEFDFVNSFHTHATFFHYYPTGTRKPGSGEISPSEFTDTKIFGQGERGILEFRYGFP
                     GKFMFHAHVSEFAELGWMGLFEVEE"
     gene            8882..9562
                     /locus_tag="AC16CM_05405"
     CDS             8882..9562
                     /locus_tag="AC16CM_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3139299.1"
                     /translation="MTDPTVEKPYSSSVGDYIKAIWELGGVGSASTKDVADRLLVSPA
                     SVSNMFVRLQEMGLAEYERYQGASLTERGRVEALRLIRRHRLIETFLLEHLGYDWQEV
                     HEEAERLEHAVSDGFTERLAQLLGHPDRDPHGDPIPSAEGTLETDDSFVLSQAIAGQR
                     LCISKVRDEDAAMLDYFGDRNLVPGRWLRIREVRSLDDVVIVEDEEGEVYALGEALAR
                     AIFVRNDC"
     gene            9607..10497
                     /locus_tag="AC16CM_05410"
     CDS             9607..10497
                     /locus_tag="AC16CM_05410"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR3139300.1"
                     /translation="MVNNGNPQDEATKAGSSGEVAVEDSPERGYPRYRIEPGERASLA
                     AIDPNETEHYHKKKQVAKELERQRRRIQGLQARLFAENERGLLIVLQAMDTGGKDGTI
                     KHVFSGVNPQGCRVSSFKRPSAEEANHDFLWRYHKSVPARGRIGIFNRSHYEDVLVVR
                     VKSLVPEEVWRHRYRLINDFERNLTNDGISVLKFYLHISRGEQKRRLQSRLDDPDKRW
                     KFSSADIKERAFWDDYQAAFEDALTNCSTEHAPWYVVPANKKWYRNLVVARTIADTLA
                     AIDPQFPPAEEGLEKVHIPD"
     gene            complement(10582..11715)
                     /locus_tag="AC16CM_05415"
     CDS             complement(10582..11715)
                     /locus_tag="AC16CM_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3139301.1"
                     /translation="MLGEDAQEGIGCGLAGEDRGMDRVLAALYGSSGGSGARSAGLGG
                     SAPNVARWLGDIRTYFPSPVVRVMQKDALERLNLRQMLLEPEMLESVEPDVHLVATLV
                     SLGRVIPEGTRDSARRVVRKVVEELERRLQEKTVRAVRGSLDRVARTTRPRPADIDWD
                     RTIRANLKNYQPEHRTIIPEKLLGNARRRKSAALRDIILCVDQSGSMASSVVYSGIFG
                     AVLASIRAVNTRMVAFDTSVADLTEDLQDPVDLLFGVQLGGGTDINRALAYCQTLVSR
                     PTDTVLVLITDLYEGGDRDAMLKRAADLTASGVNMVCLLALSDDGAPTHNPQLAAALA
                     NLGVPAFACTPDLFPDMMAAAIQKQDLDLWAARNDIVTARGEE"
     gene            complement(11744..13930)
                     /locus_tag="AC16CM_05420"
     CDS             complement(11744..13930)
                     /locus_tag="AC16CM_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HIR3139302.1"
                     /translation="MSVRVFGIRHHGPGSARSLLTALEDMSPDAVLVEGPPDASEVLP
                     LLARPEMVPPVALLVYPSDAPQKAAFYPFAAFSPEWQALRFALGRNVTARFVDLPRSL
                     DGGDEDESSEVREDPLGLLAEAAGYEDRELWWERQVEQRSDATDLFEAITEAMTALRE
                     GHEPPPREARREAFMRREIRRALREGHENVAVVCGAWHAPALKVEDHSPKSDDALLKG
                     LRKKKTSATWIPWTTGRFSYRSGYGAGIASPGYYEHLWNASGGNVGGRWLTHIARLLR
                     SEDLDASPANVVEAVRVADTLAALRDLATPGLGELREAALATLCGGETEKLDLIREKL
                     EIGEKLGEVPTEAPIVPLQRDLERLQKRLRLKPSAEERHLDLDLREDNGRARSELLHR
                     LRLVGTDWGEPRAISRKSGTFHELWALRWRPELSVALVEASVWGNTVQSAASSRVRNL
                     AEDADLPRLTSLLDAAILARLPEESVENLLGRVRDTAASADAGLLMDALPPLARVARY
                     GDVRGTEASIVVPVVDALFERALVALPGACSALNDDAARAMANSIGGVQECVNLLDNP
                     IMAERWHATLTPLVENEGVHALVRGWSCRLLLEAGVLDEEGMRKLAGLALSPAVPAPE
                     AAAWVEGALRGSGLVLLHQDGLWRALDLWISNLDPETFTVMLPLIRRAFSGFEPPERR
                     AMGEKVRKLKDEHDDAATPERDEVDHRRAARTLPVLAQILGVDRDG"
     gene            complement(13934..15025)
                     /locus_tag="AC16CM_05425"
     CDS             complement(13934..15025)
                     /locus_tag="AC16CM_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139303.1"
                     /translation="MTDGQNAVLRRHAEQEYAEELAELARTDDRQRPPNWRLSPWAVK
                     TYLMGGRLSNGFEVVPKYVGKGRLMEMAIATLATDRALLLYGIPGTAKSWVSEHLAAA
                     VSGDSTLLIQGTAGTDEAAIRYGWNYARLLTEGPSEEALVESPMMNAMRSGTVCRIEE
                     LTRIAPEVQDTLITVLSEKALPVPELNTEVQASKGFNVIATANNRDKGVNELSSALTR
                     RFNTVVLPVPETIDEEVEIVERRVASLGRALELPAEKPAMEEIRRVVTVFRELRGGVT
                     EDGRTKLKSPTGTLSTAEAISVLTGGLASAAFYGDGTMKAGDVAGGLTGAIIKDPVQD
                     RVAWLEYLQTVVRERKGWKDLYRECREVV"
     gene            complement(15022..>16189)
                     /locus_tag="AC16CM_05430"
     CDS             complement(15022..>16189)
                     /locus_tag="AC16CM_05430"
                     /inference="COORDINATES: protein motif:HMM:NF039502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HIR3139304.1"
                     /translation="LAAQNLDWEWAVATRGSVDDESTWEEGPPEERIVALRRVREREP
                     ALGLSWVEETWKSEKADTRKAMVGALETGLSSQEEAFLERALDDRSVRVREAAATLLA
                     RLPESDYAGRAMARARTILVRYEPPAGGLRGVAAGFSRRGHTGRISVEPPEHPDAEWE
                     RDLPGAMPERGVGEKAWRISRALSVVEPARWEERFGVAPSDLISAARGDWEVPLLAGW
                     CRASVLHRSQSWAAPLWRACYGLEHDPEGWQTWQVALSMADLIPQSDLTSAITRLPKN
                     GEMPSRLSSMLQATSGPWLPELSEAFLEGLRRRVRGVCKYGLAQGEEPWVTVIPHASV
                     SLATECLRGAEGFREILEGCSGPQDHARYRWGPVLKRFDETLELRRELVEEIPL"
BASE COUNT         2991 a         5390 c         5150 g         2658 t
ORIGIN      
        1 cgacccactc ggggacgacc gtgaaatggg agatgccggg tgccgcgacc acggcgtcgg
       61 ccgtgatgcg cccgccgctc tccagcgtcg cctcgaagtg cccgtttggc ctggagaggt
      121 cccgcacgag gtcgggaaca acctgaaggt cctccctctc ctcgaaccag tccacatagt
      181 ccagaaatag cccaaccggg atgggatcga aatccgcggg gtcgatccca cgctcctcca
      241 gatacgcctc gaaggtgttg acctcagccg cgtcgaggtg ccagtcggaa ccggagcgca
      301 ggaacatacc cgccggcatg ttctcccgcc agaaggccat gggctccccg accacgattt
      361 gctcgatgcc agcatgcctg gcgcacgcgg cggttgccag cccgtacggt cccgcgccta
      421 cgaccaacag cggtacatgc gtgtgcgtat gctcttctac ggcttcgggt ctcgacgcgt
      481 gcacgggcta acctcctgta tccatgaacc atccaccagg aactgtaaag aactttacat
      541 cttcagatct gtcatatctc acccagaccg taagaggccg aatcggcttc ttcaccgtag
      601 cccggaaagt accctctcgt gggcttcgag gtctctctcg ccgaagagca cgaaccgcac
      661 caggcgtacc acttcgagcc gttccgcctc ctcgcgcacc gtctgcagcg cggtctccgc
      721 cgcctcttcc accggatacc cgaatatgcc ggttgagatg gcgggaaaag cgatagatgc
      781 cacaccattg tcctcggcta gggccaggga gttacggtag cagcgggcca ggagcaccgg
      841 ctcgggacgg tcctggccgt agacgggtcc cagcgtatgg atcacgtatc cgttgggaag
      901 cccatgcgcg ccggtaatga cggcctcgcc cggctcaatc gggccgagtg ggacggcttc
      961 ttcggcgaga cccggtccgg cggcgcggtg gatggccccg gccacgcccc caccagatgc
     1021 gagcctggcg ttcgcggcgt tgaccacggc ctcgacgtct ccctgggagg ttatgtcccc
     1081 ccggatgcac tcgaccgtca agtcaccaag ctctcgtctc acatgccctc cctggagtag
     1141 gccagcccgc cgccccgcca gacggcctgc acggagaggt cgggggccag gaccgtcacg
     1201 tcggcgtcgt aacccgagac gaggcggccc ttgcgacggc cctcgcctat caacctcgcg
     1261 ggggcggtcg aggtcatgcg cgccgcctcg gggacggtgc agccggtgaa ggccaggatg
     1321 ttcttgaagg ccccgcgcat ggtgagcacg ctgcccgcga tggccccgct ccccagcttc
     1381 gggacgccgc cgtcgacgta gacggtgcgg gtggcgaggc ggtattcgcc ctcctccatg
     1441 cccgcggcgg ctatcgcgtc ggtgacgagg cagatacggt ctgggccgag catacgaaag
     1501 gcgagcccca ccatctcggg gtggacgtgc agcccgtcgg ggataaggcc gcacaccgcc
     1561 cgcggatggg cgaacgcggc ccccggtagc cccggctcgc gatggtgcat ggagctcatc
     1621 gcgttgaaga ggtgtgtgac gccggcggca ctcccgtcca gcgcaccgta ggccggttcg
     1681 aaggccacgt cggagtggcc ggcgcagacc accgccccgc ggctcgaggc aaggtccatg
     1741 agcgcggtcg cgccctccag ctcgggcgca agcgtaacca ttcggaccgg accgagatcc
     1801 aggagtcggg cgagcaactc cgtgtctggc ctcacgacgt gggccgcggc gtgggccccc
     1861 cgcttcgaca ggtttatgaa gggtccttcc aggtgaaccc cgagcacctc ggcgccagac
     1921 cgccttcccg aggccgcggc ttcgatggct ggtagggcct cctcgtagat gctctcaggc
     1981 gatgatatga ccgtgggcag ataggaggtg gtaccggtcg agagcagggc cctggagagc
     2041 tcggacagcc tgccgctctc cgaggccacg tcgatgccga aggcaccgtt cacctgcagg
     2101 tcgacgaagc cgggtaggac cagggcctcg ccgtagtagt ggacctcgtg cgcgtcggcg
     2161 gcgagcgagt cgtccgggct cacgcccgcc acggagctgt cctccaacag tacggttcca
     2221 tccggccaga cctcgtagtc cgtgacgacc tgacccctga gcgccaccag gctcatgaac
     2281 cctccaaacc gctctgcctc atcatagggt gcatcttctg ctttccggac cgatggatca
     2341 agggctccta ctaactccgc ggtggccctg ggggaccgac gacctcggtg ccgtaggcgg
     2401 caaggtcccc ggccatctct tccaggaggt cctggtctag ctctcccggc gggggaagcg
     2461 cgaggtccct ggccggctcg ctgaacctgg aatcctggaa gtgggcctcc agcccaccag
     2521 gcgcgatgaa ggagagcata cggacaacgt cggtctcgaa agcgtaggaa tggggcacgc
     2581 catgtggcag gaagacgaac gtgccaggat cggcaaggta ggtttcctcc ccgacgtaga
     2641 agatgaccct accgtcgagc acgtagaagc cctcgtcgtc gctgtggtgg aggtggcgcg
     2701 atggctcgcg gccccttggc accaccatct cgatcaaccc gaaacgtccg tcggtctgtt
     2761 cggcatccgc caggacgctg aaaagacagc ccatgtagga gaccgaagac tccgagtcca
     2821 tacggcgagc gtaccgcgat gcccccggat ctccctgtag ttcccttgcc atgcacgacc
     2881 tcctttcccc gccatgatcc acagtataga cctggtgttc gagaggctcg tgtccctgcg
     2941 gcctcttcgc accctccccg accgtcgggc atgggggtgt atcatggacg attagaccga
     3001 tggttcgttg aagaggggtc catgacagca cagggcagcg acacgggttg gtggtggccg
     3061 ggctggcgat catagtcaga gcggtcacga ggacctgaac ggaatgtgat acgggccgtt
     3121 ctcagggtga tgctggcagt gtgcctgttt tctatgctcg ccgcctgctc ggaggacccg
     3181 gccggacagc ggaacggagg tcagggttcg cccgtcccgt cgggcacgtc cgcgagcgtc
     3241 gcttcttccg gaacgaacac ctctccgacg accgagacgg cggagcccgc agagggcccc
     3301 gtggtcgaga gcaccgtggg tggcgcggtc ctgcgcagag acgagagcgg cttgaaaatc
     3361 gtccaggcca ggctgctgcg cgcgaaggag aacgacgaga tattcgtgaa ggccagggtg
     3421 gagagaccgg cggaagcgag agactgctac ctcatggagg cttcgacctg gttcgacctg
     3481 cgcgacgcga tagagagtga gaacatctct gatgtgcctg acgtgctaga ggtgctctgg
     3541 gcggatcctt catcgacgac caggttctcc ggtggcgaca cgcttgccat ctcctttccg
     3601 gagaacacag accggaaagc cggggggccg gacaccgagc acacgtcgtt cttcgtggtg
     3661 tgctacaaga ccgccaaccc cgaaagcaac gtgacgtggt acgacgtggc ccacgtcgaa
     3721 ggcacccccg aagtgccgta ggaggctaca ggccccgggc ttcaggaata ttgcccgttg
     3781 tttgaacagc cggggcctat ccgagggcca catcgagggt catcatgacg gcgaagccga
     3841 ccatcaggga gagggccgcg atgtctgggc tgccgcgttt ggcctcgggg atgagctctt
     3901 ccgccaccac gaagatcatg gctccggcgg cgaaggccag cgcgtagggc aggatgggct
     3961 gggcgatgag cacggcagac gccccgataa cggccgcgat aggctcgacg acccccgaaa
     4021 gttgaccgta gaagaagctg cggccgcggc tcatcccctc gcgccgcagc ggcatggaga
     4081 ccgccgtgcc ctccgggaaa ttctgcagcc cgatgccgat ggccagcgcg atcgccgccc
     4141 cgagcgtagc cccggggaca ccttccgtaa cgcctatacc aaccgccccg aaggtgacgc
     4201 ccacggcaag gccctcgggg atgttgtgca gcgtgatcgc ggacaccagg agcacgctgc
     4261 gccgccagct ggtgtggata ccctcagctt cggacagctt caagccaggg tgcaggtggg
     4321 gcagcaaccg gtcaaggagc cgcaagaaca ccccaccggc caggaagcct ataaccggcg
     4381 gcacccaaac aggcagcgaa cccgcctccg caagctctat cgccggcgcg agaagcgacc
     4441 agaagctggc cgcgatcatt accccgcccg cgaacccgag catcgcgtca agtagcttgc
     4501 gcggcacgtt cttggttccg aacaccagcg ccgccccagc cgccgtcatg ccccacgtga
     4561 acaacgtcgc cagaaacgcc tgcaagaccg ggggcatccc ggtaagaacc ccgatcatct
     4621 tgcgtacccc ctccggcgaa tcttgttgcc gtcgaacaac aggcttcctc taacatcgaa
     4681 atacttcacg acttaacctc ccaagaacgt cttgtacagg agaaaaacat tgagcgacac
     4741 gataagggcg accacgacag acgcgagggc cgtggtgacc ttgtagttga cgagggtccc
     4801 catcacgtcc ctgttgcggc agaagatgag cagcggcacg agcgcgaacg ggatgccgaa
     4861 cgagagcacg acctggctta ggaccagcga acggctgggg ttcaggccga tgccgaggat
     4921 gatgagggcc ggagccatgg ttatgaggcg gcgcaagaac aacggtatct gcctgttgac
     4981 aaaaccctgc atgacgacct gaccgctcag ggtgccgacg gaggaactgg agagcccgga
     5041 tgccagcagg gcgatcccga agaggatggc cgggatgctg ccttcgagga tgcgaagctg
     5101 ctcgaaggcc ttgtcgatat cgccgacgcc ggttaggccg gccgagtgga agagggccgc
     5161 ggccatgatg agcatgctcg cgttgatggc cccggctatg gccatggcga tgatcacgtc
     5221 taccagctcg aagcggaaga tcctgcgctt ctcctgctcg gtcttgccca cgatcctgcg
     5281 ctgcgtgagc gccgagtgca gatatatgac gtgcggcatg accgtggccc cgagaatccc
     5341 agccgccaag aggacgctct cggtaccagc gaaccccggc gtgaagagcc cggccagtat
     5401 cctgtcggcc tcgggctccg cgtagaacat ctggaaggcg aacgccacca cgataacccc
     5461 gaccatcgcc gctatcacgg cctccagcgg cctgaacccc cgccgctgca actcgagtat
     5521 cccgaacgcc cccacggccg taagcaggcc agcagggaag agcggtatcc cgaagagcag
     5581 gttgagcgcc agcgccgccc cgataaactc ggccagatcg gtagccatcg cgatgatctc
     5641 ggcctgcacc cacagcaaga atgtaaccgg ctttggaaaa cgcgcccggc acacctcggg
     5701 caggttcttg cccgtagcta tccccagctt ggccgacatc gactggatga gcatggccat
     5761 caggttggag accaggatca cccacaacag cagatacccg aactgcgccc caccagcgat
     5821 gttggtcgcg aagttccccg gatccacgta agccaccgca gcgatgaacg caggcccgag
     5881 aaagggcagg atcttgcgta tcccccgccg ttttcccctc aacgattcgt gcgcagcccg
     5941 cgccgttgcc gtctctccgg gaagtacccc ttcgactcct cttatctctt cgacccggtt
     6001 cttcacataa tcttctccac gatcatctta atttttaggc ttggctaatt gcgaagtgta
     6061 ccagacctaa aaggaatcta tgggatctct cgagttcggg cacgacctct cgggctcgaa
     6121 cttgccagcc tggcctacac gctcaccagg agggcggtca cgtacatgat cgcgatcccg
     6181 gtggtgagac ctccgagatt cgtcgccgag aatacgggcg tcttcgagtg ggccgagtct
     6241 ctcatgagga ggcgtcccac ttcgtaaacg acctggagta tggccccgac tccgatggcg
     6301 aggaagacgg tcgagaagag gttggagtag gcgaagccgc cgatccaggt cccgacgatg
     6361 gccggacctc caccgagcag cgcgagcgcg ccgaagtgcg ccaagcttgg cctcttctcc
     6421 ttcaagaccg gcgcggcgat gccgacccct tcggtgatgt tgtggagggt gaagccgagg
     6481 atcaagaagg tccccaaagc cgcctcgccg agcgcgaagg ccgccccgat ggcgagtccc
     6541 tcccccaggt tgtgcaaccc tatccccagg gctatgcgat aggaagtcgc gagcctgcca
     6601 acctccccac gccggaacag ccgctcgaca ccgagcagcc cgaacagcgt caagagggcg
     6661 acgaggataa ccaggggcac cccagaaaaa gcacccggca gcccgccggc gatctccacg
     6721 gcctcgagca gcgtgtcgag cgccagaaag accagtaggc cgaccgtcag ggcgaggacg
     6781 aagttcatgc ctgcgctacc aaggcgccgg aggaacgggt accacagaag cccgagcgaa
     6841 acgggcacga cgcccacata gaaccctatc agggcgtatt cggagaaggt ctctccggag
     6901 aagccagggg tcagtacggc gacgggtacc tcagcgtcaa acgtcgtccc ggtctcgctg
     6961 atcagcacga tctcgtacgc ctctccctcc acccacatgt aggggataac aacttccgcc
     7021 gagccgagcc gcgggatcgt tccgtcgggc tcgacccgaa agtcccacag cgcgtcgttg
     7081 accactacct gttccagggt gaccgggttc ggaccgccgt tctggacgtg caccacgaac
     7141 tcgttttccg tcggcagggt cacgcgacca acggtgaggt cctctacggg cggtgcgtcg
     7201 ctgcggccca cccccacccc gtagatgaag atcgatgcca gcaacaatcc gagccccaga
     7261 agtggtagga ggccgagaag cacccaggcc ggcccgcgct tttcgcgccc catcccctac
     7321 tcctctacct cgaacaggcc catccagccg agctcagcga actcgctgac atgggcgtgg
     7381 aacatgaact tgccgggaaa accatagcga aactccagga tcccgcgctc cccctggcca
     7441 aagatcttgg tgtccgtgaa ctcggagggc gagatctccc cgcttcccgg tttcctcgtg
     7501 cccgtcggat agtagtggaa gaaggtggcg tgcgtgtgga agctgttgac gaagtcgaac
     7561 tcgagcacgt tgaccgcgta cacccggatc aaacggtcct tgactatctg gatgggatgg
     7621 cgctggtagt ggaaggccac ggtgtttacg gcgtacacct cgttagaggc gtcgaagttg
     7681 gtgtcgaagc cgttcatgac catgaccatc tcgtcggctt cgggacggcc ttccttggga
     7741 tctatgatga acgcgccgta gaggcccttt tcgatgtgct tcctgagcgg cgccgtgtgg
     7801 caatggtaga gatgcagccc ggccggctcg gcgtcgaatt cgtacacgaa ctcctgcccc
     7861 gtccctacaa gctcgaagac accgtccatg ttggcagggt gaaatccgtg gaagtggatg
     7921 gtgtgggggt gcacgctgtt gttcttgaag cggatgcgga cccgctcccc ctccgtagcc
     7981 ctcagcgtgg gaccgggcac ctgcccgttg taggcccagg ccggatacac gataccggga
     8041 gcaacctcta tctcggtctc caccgccgtc agctcgtact ccctgacggt cctcccgccc
     8101 tctttccgct cctcgcccct gtagaagtcc ctcaagaacc tggtcggatc gaatcgcgag
     8161 agattcacat ctccgacaac cccatttccc ccgtggaaat tgtgcccgac gccagagaca
     8221 tgccctccct gatggtgcgc ctcctgcccg agtgcccgct cgacgtgcgc gccaagcacg
     8281 gcgccagagg cgccgaaagc ccccacagcc cccgttaccc tcaggaagtc ccggcgcgag
     8341 agagcgccag ccttcccccc accctccaaa aacgccacct ccgccccccg aacgggcacc
     8401 tctgctccca tcctgtagag cacagccagc gtgacgacga gcgctacctc acaaatcgtc
     8461 gcggtgagac ccaggacccc caccgcctcg acctccccaa cgtgcggccc gaagggcaca
     8521 cccaccgtgc gcgtgacgag atagaacgcg acgatggcaa ggttgccccc catgccaagg
     8581 atgaagaccg tcttgacagc ccatcgccac aaagccacac cgtagaaacc ctgcagagcc
     8641 gccaccagca agaacaggat cccgtacaac caccactctc cgaaatgctc gggcatggcc
     8701 cagacgtgga tcatcgccgc gcccagagtc aatgctgccg ccatccccaa caaagttctt
     8761 cgagcgccca tgccaaccac cactggattt tttagcttta cctaattttc aatatagcat
     8821 aagctaataa ccaatgcgac acaccgagcg aaacgaggca ggtctataat gtcctttact
     8881 catgactgat cccacggtgg aaaagcccta ttcctcgtcg gtcggggact acatcaaggc
     8941 catttgggaa cttggtgggg taggcagcgc ctcgacgaag gacgtcgcgg acaggctact
     9001 ggtctcgccg gcttccgtct ccaacatgtt cgtgcgtttg caggagatgg gtctggcgga
     9061 gtacgaacgc taccaggggg cttcgttgac ggagcgcggg cgggtagagg cgctgaggct
     9121 gatacgccgc cacaggctca tcgagacgtt tctgctcgag cacctcggtt acgattggca
     9181 ggaggtacac gaggaggccg agcggctgga gcatgcggta tctgatgggt tcaccgagcg
     9241 tctggcgcaa ttgttggggc atcctgatcg cgacccgcac ggggacccga tcccttcggc
     9301 ggaggggacc ctggagacgg acgactcttt tgtcctgagc caggccattg cgggccagcg
     9361 gctctgtatc tccaaggtca gggacgagga tgctgcgatg ctcgactact tcggcgaccg
     9421 caacctcgta ccggggagat ggttgcggat cagggaggtg cggtccctcg acgacgtcgt
     9481 gatagtggag gatgaggagg gggaggtcta cgctctgggc gaagcgctgg cgcgagcgat
     9541 cttcgtcagg aacgactgct agcctcgcga tcttgtccgg aagaaccaga cgggagaagg
     9601 agtatcttgg ttaacaacgg caacccgcag gacgaggcca ccaaggcagg atcgtcgggc
     9661 gaggtagcgg tcgaggattc gcccgagcgg ggctatccgc gttacaggat cgagcccggc
     9721 gagcgggcat ctctcgcggc aatagacccg aacgagaccg agcactacca caagaagaaa
     9781 caggtggcca aggagctgga gcggcaacgc aggcgcatac agggccttca ggcgcggctg
     9841 ttcgcggaga acgagcgggg gcttctcatc gtgcttcagg ccatggacac ggggggcaaa
     9901 gacggcacca tcaagcacgt cttcagcggc gtcaacccgc aagggtgcag ggtgtcctcc
     9961 ttcaagagac caagcgccga ggaggccaac cacgacttcc tgtggcgtta ccacaagagc
    10021 gtgccggcga ggggtaggat cggcatcttc aaccgctccc actacgagga cgtgctcgtc
    10081 gtgcgcgtca agagcctcgt gccagaagag gtgtggcgac accgctaccg cctgatcaac
    10141 gactttgaac ggaacctgac gaacgacggc attagcgtcc tgaagttcta cctgcacatc
    10201 tccagaggcg aacagaagcg gcggcttcag tcccgcctcg acgatccgga caagcgctgg
    10261 aagttctcct cggctgacat caaggagcgc gccttctggg acgactacca ggccgccttc
    10321 gaggacgccc tcacgaactg ctccacagaa cacgccccgt ggtacgtcgt tccggccaac
    10381 aagaagtggt accgcaacct ggtggtagcc cgcaccatag ccgacaccct tgcggcgata
    10441 gacccgcaat ttcctcctgc cgaggaaggg ctcgagaagg tacatatacc ggattgagac
    10501 ggtctttcag gcagagaacc agagaggaag gtttcagact tcgggaagat cacctgtagc
    10561 cgtctcttcg agacggccgc cctactcttc gccacgggcg gttacgatat cgttgcgggc
    10621 ggcccagagg tcgaggtcct gcttctgtat ggcggcggcc atcatatcgg ggaagaggtc
    10681 gggggtgcag gcgaatgcgg ggacgccgag gttcgcgagc gctgcggcga gttgcgggtt
    10741 gtgggtgggt gcaccgtcgt ccgagagggc gagcaggcag accatgttga cgccgctggc
    10801 ggtcagatct gcggcacgtt tcagcatcgc gtcgcggtcg ccgccctcgt agagatcggt
    10861 gatcaggacg agcacggtat ctgtggggcg ggagacgagg gtctggcagt aggcgagggc
    10921 gcggtttata tcggtgcccc cgccgagttg gacgccgaag aggaggtcga cgggatcctg
    10981 gaggtcctcg gtcagatcgg cgacggaggt atcgaaggcg accatgcgcg tgttgacggc
    11041 cctgatcgag gcgagtacgg ccccgaagat gccagagtag acgacggagg aggccatcga
    11101 gccgctctgg tccacgcaga ggatgatgtc acgaagcgcg gcgctcttgc gccggcgggc
    11161 gtttccgagg agcttctcgg ggatgatggt gcggtgctcg ggctggtagt tcttcaggtt
    11221 ggcccggatg gtgcggtccc agtctatgtc tgcggggcgc ggccgggtcg ttcttgccac
    11281 ccggtcgagg ctaccgcgca cggcgcgcac cgtcttctcc tgcagccgcc gctccagttc
    11341 ctctaccacc ttgcggacca cgcgccgggc ggagtctctg gtcccctccg ggatgacgcg
    11401 accgagcgag acgagggtgg cgaccaggtg tacgtctggc tccacggact cgagcatctc
    11461 gggctccagg agcatctgtc gcaggttcag cctctcaagg gcgtccttct gcatcacgcg
    11521 cacgaccggc gacggaaagt acgtccttat gtccccgagc cagcgcgcga cgttcggcgc
    11581 gctgccaccg agccccgcgg agcgcgcccc tgaaccaccc gatgagccgt acagggcggc
    11641 gagcacgcgg tccatccccc ggtcttcccc ggcgagcccg cagccgatgc cttcctgcgc
    11701 gtcctctccc agcatcagcc gccagcggcg caggcgctcg agctcatccg tcacggtcca
    11761 cccccagtat ctgcgcgaga accggcaacg tccgggcggc ccgacgatgg tcaacctcgt
    11821 ccctctccgg tgtggccgcg tcgtcgtgct cgtctttcag tttccggacc ttctcgccca
    11881 tcgcccgtct ctcggggggt tcgaagcccg agaaggcgcg acggatcagg ggaagcatga
    11941 cggtaaaggt ctcggggtcg aggttcgaga tccagaggtc cagcgcccgc cacaggccgt
    12001 cctggtgcag gagcacgagg ccgctcccgc gcaaagcgcc ctccacccag gcggcagcct
    12061 cgggtgcggg tacggcaggc gagagcgcga gccccgccag ctttcgcatc ccctcttcgt
    12121 cgagaacacc ggcctcgagc aacaaccggc agctccaacc acgcaccaga gcgtggacgc
    12181 cctcattctc cacgaggggc gtcagggtag cgtgccacct ttcggccatg atggggttgt
    12241 cgagcaggtt cacgcactcc tgtacaccgc cgatgctgtt ggccatcgca cgcgccgcgt
    12301 cgtcgttcag ggcggagcac gccccgggaa gggctacgag cgcgcgctcg aagagggcat
    12361 ccaccacggg cacgacgatg gaagcctccg taccccgcac gtcgccgtag cgggcaacgc
    12421 gcgcgagcgg gggcagggca tccatcagaa gcccggcgtc ggccgaagcc gccgtgtcgc
    12481 gcacccggcc gagcaggttc tccaccgact cttcagggag ccttgccagg atggcggcgt
    12541 ccagcaagga ggtgaggcgg ggcaggtcgg cgtcctctgc gaggttgcga acgcgtgatg
    12601 aggcggcgga ctggacggta tttccccaaa cgctggcctc gaccagggcc acggagagct
    12661 cgggacgcca gcgtagggcc cacagctcgt ggaacgtgcc gctcttgcgg gagatggcgc
    12721 gcggctcgcc ccagtctgtg ccgacgaggc gcagacggtg caacaattcg ctgcgggcac
    12781 ggccgttatc ctcacgaagg tcaaggtcga ggtggcgttc ctcggcggaa ggcttgagtc
    12841 gcagcctctt ctgcagcctt tccaggtcgc gctgcagggg cacgatagga gcctcggtgg
    12901 gaacttcgcc cagcttctcg ccgatctcca gcttctcccg gatcaggtcc agcttctccg
    12961 tctcgccccc acacagcgtc gccagggcgg cctcgcgcag ctcaccgagg ccgggggtcg
    13021 cgaggtcgcg gagcgcggcg agcgtgtccg ccaccctgac ggcctcgacc acgttggccg
    13081 gcgaggcgtc gaggtcctcg gaacgcagca gacgggcgat gtgggtgagc caacgcccgc
    13141 cgacgttgcc gccggacgcg ttccagaggt gctcgtagta gccgggcgag gcaatgcccg
    13201 cgccgtaacc agagcggtac gaaaagcggc ccgtggtcca ggggatccag gtggccgagg
    13261 tcttcttctt tctgagcccc ttcaaaaggg cgtcgtccga cttcgggcta tggtcctcta
    13321 ccttcagcgc gggagcgtgc caggccccgc acacgacggc cacgttttcg tggccttccc
    13381 gcagggccct gcggatctca cgcctcataa acgcctcgcg cctggcctcc cgcggcggcg
    13441 gctcgtggcc ttcgcgcagc gcggtcatcg cctccgtgat ggcttcgaag aggtcggtgg
    13501 cgtcgctgcg ctgctccacc tgcctctccc accacagctc ccggtcttcg tatccggcgg
    13561 cctcggcgag gagcccgagg gggtcctccc tgacctctga gctttcgtct tcatcacccc
    13621 catccagcga acgaggcagg tccacgaagc gggccgttac gttgcggcct agcgcgaacc
    13681 ggagtgcctg ccattcgggc gagaacgcgg cgaaggggta gaaggcggct ttctgggggg
    13741 cgtctgacgg gtagacgagc agggccaccg gcggcaccat ctccggcctg gcaagcagcg
    13801 gaagcacctc cgaggcgtcc ggtggaccct cgaccagaac ggcgtcgggg ctcatgtctt
    13861 cgagtgcggt gaggaggctt ctggcgctac cggggccgtg gtggcggatg ccgaagacgc
    13921 ggacgctcac cgttcagacg acctcgcggc actcgcggta caggtccttc cagcccttgc
    13981 gctcacggac cacggtctgg aggtactcca gccacgccac gcggtcctgg acggggtctt
    14041 tgatgatggc gcctgtcagg ccaccggcca catctccggc cttcatcgtg ccgtcgccgt
    14101 agaaagcggc gctcgccagg ccgccggtaa gaacgctgat ggcctccgcg gtggagagcg
    14161 tacccgtcgg ggacttgagc ttcgtcctgc cgtcctccgt cacgccgccg cgcagctcgc
    14221 ggaacacggt tacgacgcgc cggatctcct ccatagccgg cttctcggcg ggcaactcca
    14281 gcgcccgtcc cagggaagcc acgcgccgct ccactatctc gacctcttcg tcaatcgtct
    14341 cgggtacggg caagacgacc gtgttgaagc ggcgcgtaag ggcgctagac agctcgttca
    14401 cgcccttgtc tcggttgttg gccgtggcga tcacgttgaa gcccttgctg gcctggacct
    14461 cggtgttcag ctcgggcacg ggcagggcct tctcggagag gacggtgatg agggtgtcct
    14521 gaacctcggg cgctatgcgg gtcagctcct cgatgcggca caccgtgcca gatcgcatag
    14581 cgttcatcat cgggctctcc accagagcct cttccgaggg accctcggtc aggagcctcg
    14641 cgtaattcca gccgtagcgg atggcggcct cgtccgtgcc tgcggtgccc tgtatgagga
    14701 gcgtggagtc gcccgagacc gccgccgcga ggtgctcgct cacccacgac ttggccgtgc
    14761 cggggatacc gtagagcagc agcgcccggt cggtggctag cgtggctatc gccatctcca
    14821 tcagacgccc tttgcccacg tacttgggca cgacctcgaa cccgttggac aaccttccac
    14881 ccatcaggta agtcttgacg gcccacggcg agagcctcca gtttggcggc cgctggcggt
    14941 catcggtcct ggcgagctcg gccagctctt cggcgtattc ctgctcggcg tgccttcgca
    15001 gtacggcgtt ctgtccatcg gtcacagcgg tatctcctcc acgagctcgc gtcgcaactc
    15061 caacgtctca tcgaacctct tcagcaccgg cccccaacga tacctggcat ggtcctgcgg
    15121 gccagaacaa ccttcgagta tctcccggaa accctcggcc cctcgcaggc actcggttgc
    15181 caacgatacg gacgcgtgcg ggatgaccgt tacccaaggt tcttcgccct gcgccagccc
    15241 gtacttgcag acaccccgaa cccgccgtcg gaggccttcg agaaaagcct cggacaactc
    15301 cggcaaccac gggccggagg tagcctgcaa catagaagag agacgagatg gcatctcacc
    15361 gttcttcggc aaccgcgtga tggcggatgt gagatccgac tgcggaatca gatccgccat
    15421 agacagcgcg acctgccagg tctgccagcc ttcaggatcg tgttcgagcc cgtagcacgc
    15481 ccgccagagt ggggccgccc aactctggct tcggtgcaac acgctcgccc ggcaccatcc
    15541 cgcgagcaac ggcacctccc agtcgccgcg cgcggcggag atcagatcgg acggcgccac
    15601 gccgaagcgt tcctcccagc gtgcgggctc taccacagat agcgcgcggg agatcctcca
    15661 cgctttctcc ccaaccccac gctccggcat tgcacctggc aggtcgcgct cccattcagc
    15721 gtcgggatgc tcgggtggct cgacggagat cctgccggtg tgtccccggc gcgagaaccc
    15781 agcggccacg ccccgaagac cgccagcggg gggctcgtag cgaaccagga tggttcgagc
    15841 gcgggccatc gcccgcccgg cgtagtcgct ctcggggagc cgggcaagaa gcgtggcggc
    15901 ggcctcgcgc acccgtacgc tacggtcgtc gagggccctt tccagaaacg cttcctcttg
    15961 cgaagacaat ccggtttcga gcgcccccac catcgccttc cgcgtatcgg ccttctcgga
    16021 cttccaggtc tcctcgaccc acgagagacc cagggcaggt tcgcgctcgc gaaccctgcg
    16081 cagcgcaaca attctctcct ccggcgggcc ctcctcccag gtgctctcgt catccaccga
    16141 tccacgggtc gccaccgccc attcccagtc gagattctgc gcggcgagc
//
LOCUS       DBNGVD010000195         8909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2955771, whole genome shotgun sequence.
ACCESSION   DBNGVD010000195 DBNGVD010000000
VERSION     DBNGVD010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2955771"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="AC16CM_05435"
     CDS             complement(<1..359)
                     /locus_tag="AC16CM_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3139305.1"
                     /translation="MFGLTQEELASRSGLSPNAVSALERGQRRRPYPHTVRALADALE
                     LSEKERATLLAAVPRREEPAPSGSPDAIPPAPRLPRPVTQLVGREREVGKVVALISRP
                     GTRLLTLTGVGGVGKTRL"
     gene            complement(536..1021)
                     /locus_tag="AC16CM_05440"
     CDS             complement(536..1021)
                     /locus_tag="AC16CM_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3139306.1"
                     /translation="MKRIATIATASMLMVSLTTGAAFASTIVGNPWSDTLKGTSGSDT
                     ISGKGEGDTLIGRGGDDSLYGNKGNDGLYGGPGDDEIHGGPGDDRAEERSVHGGEGAD
                     TLHGDAGGDALYAGPGNDTIHSVGDNAGDLVDCGSGTDTVWRGDDLKLDRFVDCERFV
                     G"
     gene            complement(1094..1582)
                     /locus_tag="AC16CM_05445"
     CDS             complement(1094..1582)
                     /locus_tag="AC16CM_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139307.1"
                     /translation="MGTEGRTDVNVGPGEGRAVRMPGQQIYTRKVGGGQTGGAFSLFE
                     VEVGPGGGDGPHVQHREDECFYVLEGRFEFAVEGAGIEALPGTLVYASRGELHSFENV
                     GEAVGRLLVLHTPGGPHERFVSEVGGPAAKGSAPREQEASEAGRLLRSAAEYGIELMT
                     PP"
     gene            complement(1793..2854)
                     /locus_tag="AC16CM_05450"
     CDS             complement(1793..2854)
                     /locus_tag="AC16CM_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3139308.1"
                     /translation="MKAKEEVRVATADLISRARGGDDAAFRELTEPHRRELLVHCYRM
                     LGSFQDAEDALQDTLLSAWRGLGGFEGRASIRTWLYRIATNRCLNALRSARRRPAREW
                     DNPGVEPPEPTRLGEVVWLEPYPDALLRGVADAPPGPEDRYEQTEAISLAFVTALQIL
                     PARQRAVLILREVLGYRASEVAGMLDSTVDAVNSALKRARAGLHRRLPPSGEPSPTPH
                     SPAERALAARFAAAYEAGDVDALIALLTADVRVAMPPIPFEYHGRDAVARFFAAVIDR
                     GRPCNLVPTRANGQPAFGVYAPTPAGDPGHGTGLYVLTFAGERIRGITRFDPDLLPRF
                     GLPRTLPTPDRRDLDPPQG"
     gene            2974..3402
                     /locus_tag="AC16CM_05455"
     CDS             2974..3402
                     /locus_tag="AC16CM_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR3139309.1"
                     /translation="MGDFNAGVIEEFRANGGRVGGPFEGYPLILIHHVGARTGVESVT
                     PVGCFARSDDRLVIIASNGGARDNPDWYYNVKAHPEVGVEFGAEAFVVEVRELEGEER
                     ERAWAGVVEEVPAVADTQNETSRTIPVLLLTRKTPHRRDR"
     gene            3425..4171
                     /locus_tag="AC16CM_05460"
     CDS             3425..4171
                     /locus_tag="AC16CM_05460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3139310.1"
                     /translation="MSDLSGRNTVVVGASRGLGRGIAREFAEAGAPVVAIARSAPALA
                     ELASTSANIRTEVADATDATEAWSLLDRYEPEVLVLVAGANPVMRPLQHQTWETFSAN
                     WHADVRIAFTWLREVLLQPLPPGSRVVVVSSGAAINGSPASGGYAGAKATQRFIAAYA
                     QDESSRAGLGITVTSVMPSMTPHGDVGRRGVRAYAARSGQPERVFLEQLGEPLTSKTA
                     GSALVDLVKADPATVAREYLLTAAGLEKLM"
     gene            4562..5005
                     /locus_tag="AC16CM_05465"
     CDS             4562..5005
                     /locus_tag="AC16CM_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3139311.1"
                     /translation="METRAGTGTSVRATGGCLCGAVRYEVRGPLRDVVACHCSQCRRT
                     SGHFGAFTATRSGDLVLTEEEGLRWYRSSESARRGFCGVCGSNLFWEPDSGGRVSIGA
                     GTLDLPTGLATAAHVFVEDAGDYYTIADGLPQRADGDHGVEIPGI"
     gene            5282..6781
                     /locus_tag="AC16CM_05470"
     CDS             5282..6781
                     /locus_tag="AC16CM_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3139312.1"
                     /translation="MSGHAVVVAGGGPTGMMLAGELALAGVDVAIVERRASQELVGSR
                     AGGLHARTIEVLDQRGIADRFLAEGQKAQVAGFASTPLDISDFPTRHPYGLGLWQNHI
                     ERILAGWVEELGVEMYRGCEVTGFAQDDTGVDVELSDGRSLRAEYLVGCDGGRSRIRK
                     MAGIEFPGWDPTTSSLIGEVQMVEEPELGLRHDELGRHALGRSEYEILDGEVVFAKEG
                     PVQVMVTERQPPPTGEPTLDDLSQALITVYGTDFGAHSLTWISRFSDTTRQAAAYRNG
                     RVLLAGDAAHVHAPDGGQGLNTGVQDAVNLGWKLAQVVNETSPESLLDTYHAERHPVA
                     ARVLRNTMAQVALRRQESRIEAVGEVVSELLGMEEPRKRYAAMMSGLGIHYDLGEGHP
                     LLGRRMPDLDLVTAEGPLRVFTLLHDARPVLLDLGEPGGLDITPWKDRVQLVEAEYTG
                     DWELPVLGTVPAPTAVLIRPDGYVAWVGEEGTDQGLAGALTTWFGPRGG"
     gene            complement(7027..7887)
                     /locus_tag="AC16CM_05475"
     CDS             complement(7027..7887)
                     /locus_tag="AC16CM_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3139313.1"
                     /translation="MTLFALGIVLVAAVFHATWNLLAKRVGDGGAVFVWLFGVCSVVI
                     YAPLAVVVVLVVGPHLGPVQYLFMAGSGILHLGYFVLLQRGYAVGDLSLVYPLARGTG
                     PLLATAAAIVLFGERPSLLVIFGIALITTGVFVLTSEGGSLRFSVGSGVFYGLMTGVF
                     IAAYTIWDKQAVSALLIPPLLQSWATTIVTTLLLTPVAMANRKKVLAIWRDFKLETIG
                     VGILSPLSYILVLTALVFTPVSYVAPAREISILIGAAMGSRLLSEGDSSRRLIAAAAM
                     VAGIVALALG"
     gene            complement(7981..8721)
                     /locus_tag="AC16CM_05480"
     CDS             complement(7981..8721)
                     /locus_tag="AC16CM_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3139314.1"
                     /translation="MSGGVVEGVPAYRRVGEEISARIKSGELAPGSRIPSERELAAEK
                     GISRMTARAAVGLLTRRGLVERRERSGVFVARPKIEQSLDTVAGLSDQLGARGVVPGA
                     EVLEARTVPVGESGDTEVGSRLGLSDEDAVHLVMRVRTGDGEPLVLEETYLPEGLWPG
                     LLDHDLSGSLYALARKIYGFSVADARQELEPMPLDPEAAAMLGVCADLPALRVTRTAW
                     DTEGRAIEFARDLHRGDRITFVSETHDD"
BASE COUNT         1477 a         2857 c         3047 g         1528 t
ORIGIN      
        1 aggcgggtct tgcccacgcc gccgaccccg gtcaacgtga gcagccgcgt cccggggcgt
       61 gagatcaggg ctaccacctt cccgacctcg cgctcgcgcc ccacgagctg ggtgacgggc
      121 cgggggagcc tgggggccgg cggaatggca tccggggagc cggagggggc gggctcctcc
      181 cgtctgggaa cggctgccag cagcgtggcg cgttctttct cggacagttc cagggcgtcg
      241 gccagcgccc tgacggtgtg agggtacggg cgtcgcctct ggccgcgctc gagggcgctg
      301 accgcgttcg gggagagccc agaccggctc gccagctcct cctgcgtaag cccgaacact
      361 tctcgcattc tacgcagtcg tgtgccgaac tcgccgcggc ctacagcggt ttccttgcct
      421 ggtgcctcct ccacaaatcc aaggatacca gaagcgaaag agaccgaaac agcgtacggg
      481 ggtctccccg attggagaga ccccgttctt accgctttcc taccgggcgc gggccttacc
      541 cgacgaacct ctcgcagtcg acgaagcggt cgagcttcag gtcgtcgccc ctccagacgg
      601 tgtcggtgcc ggacccgcag tccaccaggt cgccagcatt gtcgccgacg gaatggatgg
      661 tgtcgtttcc gggccccgcg tagagggcgt ccccgcccgc gtcgccgtgg agtgtgtcgg
      721 cgccctctcc accgtggacg gagcgctcct cggcgcggtc gtcgccgggg ccgccgtgga
      781 tctcatcgtc gccggggcca ccgtagaggc cgtcgttacc cttgttgccg tagagagagt
      841 cgtcgccgcc cctgccgatc agggtgtcac cttcgccctt gccggagatg gtgtcgctcc
      901 cggacgtgcc ctttagcgtg tcggaccagg gattgcccac gatggtcgag gcgaaggccg
      961 cccccgtcgt gaggctcacc atcaacatgg acgctgtagc aatggtcgct atccttttca
     1021 tctcttcggt tcctttctct gtttgcggta cagttcgtcg cgttttcctg ggcatcgtgg
     1081 ccggtctccc tggctaggga ggagtcatca gctctatgcc gtattccgcc gcagaccgga
     1141 gaagccttcc cgcctccgac gcctcctgct cccgcggggc gctccccttg gctgcgggcc
     1201 ctccgacttc gcttacgaag cgctcgtgcg ggccgcccgg tgtgtggagg accagtagcc
     1261 tgcctaccgc ctcgccgacg ttctcgaaag agtgcagctc gcccctgctc gcgtagacga
     1321 gcgtgccggg gagggcctcg atcccggctc cctcgacggc gaactcgaag cgaccctcca
     1381 ggacgtagaa gcactcgtcc tcgcggtgct gtacgtgggg tccgtcgccg cccccgggtc
     1441 cgacctcgac ctcgaagagg gagaaggccc cgccggtctg cccgcctccc accttgcggg
     1501 tgtagatctg ttgccccggc atccgcacgg cccggccctc tccgggcccg acgttgacgt
     1561 ctgtgcggcc ttccgttccc atgatttacc tcctttcccg gtgctacctc gtgtctttgc
     1621 gtgcccctac tttgccttcc gaaaaggcgg cccaccacgc acgcaaccca cacagtgtgc
     1681 ggagtgtggg aagcgtacgg gcgcgaggaa ggaccccgga cgagatcagc tttcagccat
     1741 cagcttttgg cttttcttct cttgctgacc gctgatcgct gacggctgga ggctatccct
     1801 ggggagggtc gaggtctctg cgatccgggg tgggaagcgt tcgtggcagg ccgaaccggg
     1861 ggagcaagtc ggggtcgaag cgggtgatgc cgcggatgcg ctcgccggcg aaggtcagga
     1921 cgtagaggcc ggtcccgtgc ccagggtcgc cggcgggggt cggggcgtag acgccgaacg
     1981 ccggttggcc gttcgcccgc gtcggcacga ggttgcacgg ccggccccgg tcgatgacgg
     2041 cggcgaagaa gcgggccacg gcatcccggc cgtggtattc gaaagggatc gggggcatgg
     2101 ccacgcggac gtcggcggtg agcagggcga tcagggcgtc gacatcgccg gcctcgtagg
     2161 cggcggcaaa ccttgccgcg agcgcccgct cggcgggtga gtggggggtg ggggaaggtt
     2221 cgcctgaggg cggcaggcgg cggtgcaggc cggcgcgggc ccgtttgagg gcgctgttga
     2281 ccgcatcgac ggtcgagtcg agcatgccgg ccacctcgct ggcgcggtaa ccgagcacct
     2341 cgcgcaagat gagcacggcc cgctggcggg cgggcaggat ttgcagggcg gtcacgaaag
     2401 ccagggagat ggcttcggtc tgttcgtagc ggtcctccgg gccgggcggc gcatcggcca
     2461 cgccccgcag aagtgcgtcg gggtagggct cgagccagac gacctcgccg agacgggtcg
     2521 gctcgggcgg ttcgaccccg gggttatccc actccctggc cgggcgccga cgtgccgagc
     2581 ggagtgcgtt gaggcaccgg ttggtcgcga ttcggtacag ccaggtgcgg atcgaggcgc
     2641 gtccctcgaa ccccccaagg ccccgccagg cggagagcag cgtgtcctgc agggcgtcct
     2701 cagcgtcctg gaacgatccg agcatccggt agcagtgcac cagcagttcc cgccgatgcg
     2761 gctcggtcag ctcgcggaaa gcggcgtcat ccccgccccg cgccctgctg atcaggtcgg
     2821 ccgtcgccac cctcacttcc tctttagctt tcatctctca ggttttcagt atagacaccg
     2881 cccgcgggtc ggatcgggcg gttgggggcg cccatttctt gtgctttgcg gtgtctatgt
     2941 tgttgaggac cacgattgat cgggaggtag gtgttgggtg atttcaacgc cggcgtgatc
     3001 gaggagtttc gtgccaacgg gggacgggtt ggtggcccgt tcgagggtta tccgctgatc
     3061 ctgatccacc acgtgggcgc caggacgggc gtcgagagcg tgacccccgt cggctgcttt
     3121 gcgcgttcgg acgaccgact cgtgatcatc gcctcaaacg gcggcgcacg ggacaacccc
     3181 gactggtact acaacgtgaa ggcccacccg gaggtcggcg tcgagttcgg cgccgaggct
     3241 ttcgtggtcg aagtgaggga gctcgaaggc gaagagcggg aacgggcgtg ggccggggtt
     3301 gtcgaggagg tacctgccgt ggcggatacc cagaacgaga ccagccgcac gatccccgtc
     3361 ctgctgctca cgcggaagac cccgcacagg agagaccggt aaaccacgta gggaaaggaa
     3421 gatcatgagc gatctgtcgg gtaggaacac ggtcgtcgtt ggtgcgagcc ggggactggg
     3481 gcgcgggatc gctcgcgagt tcgccgaagc cggcgctccg gtcgtggcga tcgcccggtc
     3541 cgcaccggcc ctcgccgagc tggcctctac cagcgcgaac atccgcaccg aggtcgcgga
     3601 tgcgaccgac gcgacagagg cgtggagcct gctggaccgg tacgagccgg aggtcctcgt
     3661 cctcgtcgcc ggcgccaacc ctgtgatgcg cccgctccag caccagacat gggagacgtt
     3721 ctcggccaac tggcacgccg acgtcaggat cgcgtttacc tggctgcgcg aggtgctgct
     3781 gcagccgttg ccacccggca gcagggtggt ggtggtgagc agcggggccg ccatcaacgg
     3841 atctccggcc agcggtggat acgccggagc caaggccacc cagcggttca tcgcggccta
     3901 cgctcaagac gagtcgagcc gggccgggct gggcatcacc gtcacgtcgg tgatgcccag
     3961 catgacccct cacggagacg tgggaaggcg gggggtcagg gcgtacgcgg cccggagcgg
     4021 ccagcccgag agagtattcc tcgagcaact gggggaaccg ttgacctcca agacagccgg
     4081 gtccgccctg gtagacctgg taaaggcgga cccggcgacg gtcgctcgcg agtacctgct
     4141 aaccgccgcc gggctggaga aactgatgtg aaaaggctac aggctttagg tttcaggctt
     4201 caggaatatt gcctgatgcc tgtgcctggt tacggcaata cctggcgtga agatccaacc
     4261 tctcgggcta gccgcggatt cccgctttag cgggaatgac ggtgtgggtt ggtcggagca
     4321 ggtagagggt cacacgacct gtagtctgct gtctgttgcc ttcctggcat cttttcgcca
     4381 gatctgctct tgcgacttgc tactcttgcg tcgcgatcct ggttgcgggt gggcgcttcg
     4441 cgaagcgccc ctacaagggg tctctgggca tgtagcattc ctgttttctg cctcgactgg
     4501 ccggagggtt cccggggtgg catgctcttt gccgtaggcg aacatctgta gaggagaggg
     4561 tatggagact cgggcgggga ccgggacatc ggttagggct acgggtgggt gcctttgcgg
     4621 tgctgtccgg tacgaggtgc gggggccgct acgtgacgtg gtcgcctgcc actgctcgca
     4681 gtgccgccgc accagtggtc atttcggggc cttcacggct acgcgctcgg gggatctggt
     4741 cctgacggag gaggaggggc tgcgctggta taggtcgtcg gagagcgccc ggcgggggtt
     4801 ctgcggggtt tgtggatcca acctcttctg ggaacctgat tcgggcgggc gcgtctcgat
     4861 cggcgcgggc acgttggacc tgcccacagg gctcgccacg gcggcccacg tgttcgtcga
     4921 ggacgcgggt gactactaca cgatcgccga cggtctgccc cagagggcgg acggcgatca
     4981 cggtgtcgag attcccggta tttgagagct tctagctaat agctatcagc tgtcagcttt
     5041 tttggctgat ggcttctggc gttggggggt gaagaggaag catggtggtc gtatgacctt
     5101 gtccctccat ctttattata tagcgcactg gggggcttgc cgcaagaccg gggggatggc
     5161 ggataatgcc ggccgaagcc gggagctacg gagattggag tagctagatg cgtgtgttgc
     5221 tgtctgggtg cgggctgctt ggttgcgggg agatgggcat gacttcggac ggggggtggc
     5281 tatgagtggg catgccgtcg tggtagccgg gggaggccca acggggatga tgctggctgg
     5341 cgagctggcg ctggccgggg tcgacgtcgc catcgtcgag cgccgcgcga gtcaggagct
     5401 cgtcggctcg cgagcgggcg gcctgcacgc gcgtaccatc gaggtcctcg accagcgagg
     5461 tatcgccgac cggttcctcg ccgaggggca gaaggcccag gtcgcggggt tcgccagcac
     5521 ccccctggac atcagcgact ttccgaccag gcacccctac gggctcgggc tctggcagaa
     5581 ccacatcgag cgtatcctgg ccggctgggt agaggagctc ggggttgaga tgtatcgcgg
     5641 atgtgaggtt accggcttcg cccaggacga caccggcgtc gacgtcgagc tctccgatgg
     5701 ccggtcgttg cgggcggaat acctcgtcgg gtgcgacggg ggacgcagcc gcatccgcaa
     5761 gatggccggc atcgagttcc cgggctggga cccgaccacg agcagcctga tcggcgaggt
     5821 ccagatggtc gaggagccgg agctgggcct ccgccacgat gagctcggca ggcacgcact
     5881 cggcaggtcg gagtacgaga tcctggacgg tgaggtggtt ttcgcgaaag agggtccggt
     5941 gcaggttatg gtgaccgaga gacagcctcc tccaaccggc gaacccaccc tcgacgacct
     6001 aagccaggcg ctcatcaccg tgtacgggac agatttcggg gcccacagcc tgacctggat
     6061 ctcccgcttc agcgacacga ccaggcaggc cgcagcctac cgcaacggac gagtcctgct
     6121 cgccggcgac gccgcgcacg tgcacgcccc tgatggggga cagggcctca acaccggcgt
     6181 gcaggacgcg gtgaaccttg gctggaagct ggcccaggtg gtcaacgaga cgtcgccgga
     6241 gagcctgctc gacacctacc acgccgagcg ccacccggtc gccgcacgcg tgctccgaaa
     6301 cacgatggcg caggtcgcgc tccgccgcca ggaatcccgc atagaggccg tgggcgaggt
     6361 cgtgtccgag ctactcggca tggaagagcc ccgcaaacga tacgccgcga tgatgtccgg
     6421 tcttggcatc cactacgacc ttggcgaagg tcaccccctg ctgggacgcc gcatgcccga
     6481 cctggacctc gtcaccgccg aaggcccgct tcgggtattc accctgctgc acgacgccag
     6541 gccagtacta ctcgacctcg gcgagccagg cggcctggac atcaccccgt ggaaggaccg
     6601 ggtacagctg gtcgaagccg aatacaccgg cgattgggag ctacccgtgc tcggcaccgt
     6661 ccccgccccc accgccgtcc tgatccggcc cgatggatac gtcgcctggg tgggggaaga
     6721 aggcacagac cagggactcg ccggggcact aaccacctgg ttcggaccgc gtggaggtta
     6781 gctgtcagct atcagctatt caaggcatca ggcttcaggt aattttcctg aagcctgatg
     6841 cctgcgctgg agtacagcga caccggggcg tgaagagaca atctctcggg ctagccgtgg
     6901 attcccgctt tcgcgggaat gacgggtgtg ggtaacgcgg gagtgatggg tgtgggtaac
     6961 gcaggagtga tgggtgggat gatcagactg agtatagggt cccacaacct gtagcctgat
     7021 gccttcctat ccaagtgcta acgctacgat tccggctacc atcgctgcgg cggctatgag
     7081 tcttcgggag gagtctcctt ctgagaggag tcgggagccc atggcggccc cgatcaggat
     7141 ggagatctcc cgcgccggcg cgacgtagct tacgggcgtg aagacgagcg cggtcaggac
     7201 gagaatgtag gagagcgggc tcaggatgcc aaccccgatg gtctcgagct tgaagtcgcg
     7261 ccagatggcc aggaccttct tcctgttggc catcgcgacc ggggtcagga ggagcgtggt
     7321 gacgatggtg gtggcccagc tctgtagcag gggtgggatc aggagcgcgc tgaccgcctg
     7381 tttgtcccat atggtatagg ccgcgatgaa gacgccggtc attagcccgt agaagacgcc
     7441 gctcccaacg ctgaaccgaa ggctcccccc ctcgctggtc agcacgaaga cgccggtcgt
     7501 gatgagcgct atgccgaaga taacgagcaa ggagggccgc tcgccaaaga gcacgatggc
     7561 cgccgcggtc gccagcaacg gccccgtgcc acgcgccagt ggatagacta gcgatagatc
     7621 ccccacagca tacccccgct gcaacagcac gaagtacccg aggtgcaaga tgccgctccc
     7681 ggccatgaac aggtactgca cgggccccag atgaggcccg accacgagca ccacgacaac
     7741 cgcaagcggc gcgtagataa ccaccgagca gacgccgaac agccagacga agacagcccc
     7801 cccatccccc acccgcttgg ccagcaaatt ccacgtggca tgaaaaaccg cagccacaag
     7861 cacgatccca agcgcaaaca gggtcattgt ggaggcaaca ggcaacaggc aacaggcaac
     7921 aggtgatctt cctgaagcct gatgcctgaa acctgtagcc ttactttctg cagagccact
     7981 ctaatcgtcg tgggtctcgg agacgaaggt tatgcggtct ccgcggtgca ggtccctggc
     8041 gaactctatg gcccggccct ccgtgtccca cgcggtccgg gtgaccctca gcgccggcag
     8101 atcggcgcat accccgagca tggccgcggc ctccgggtcg agcggcattg gctccaactc
     8161 ctgcctggcg tccgccacgg agaaaccgta gatcttcctc gccagggcgt acaggctccc
     8221 gctcaggtcg tggtcgagca accccggcca gagcccctcc ggcaggtagg tctcttcgag
     8281 cacgagcggc tcgccgtccc cggtgcgcac gcgcatgacc aggtgaaccg cgtcctcatc
     8341 agagagcccg agcctcgaac ctacttctgt gtcgcccgac tcccccacgg gaacggtgcg
     8401 ggcctcgagc acctccgccc cgggaaccac gccgcgcgcc ccgagctgat ccgagagccc
     8461 cgcaaccgtg tcgagggact gctctatctt gggccgagcc acgaagaccc cggacctctc
     8521 ccgcctctcc acgagcccgc gccgcgtgag caggcctacg gccgccctgg ccgtcatcct
     8581 ggagatgccc ttctcggccg ctagctcgcg ctcggagggg atcctgcttc caggcgccag
     8641 ctcaccagac tttatgcggg cagagatctc ctccccaacc cgcctgtaag cgggtacccc
     8701 ctcgactacc ccgccactca cacggcctca tcacccgtcg tcaatagcac gagaaccgcc
     8761 cacgctagta ccgcaacacc cagtagtgct tccatcatca tgacctcctt ctggtctata
     8821 ccatttggtc tataccagat gctatcaggg agaatggtgt gggtcaagcc aggaaaaagg
     8881 cttcaggttt caggaatggt gggtggatt
//
LOCUS       DBNGVD010000196         7531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1498702, whole genome shotgun sequence.
ACCESSION   DBNGVD010000196 DBNGVD010000000
VERSION     DBNGVD010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1498702"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /gene="radA"
                     /locus_tag="AC16CM_05485"
     CDS             <1..1232
                     /gene="radA"
                     /locus_tag="AC16CM_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3139315.1"
                     /translation="QKARAAGKTLALSEVPVVEGGGRMDAGVGELNRVLGGGIVPGSM
                     VLVGGEPGVGKSTLLLQVMGNLGEGCLMISGEESPRQVALSARRLGVEGRGFRVLSET
                     DLDVIEATILEERPSAVVVDSIQTLYSPELSGAPGGVGQVRECAARLMRLAKSEGIAM
                     VLVGHVTKDGSIAGPRVLEHMVDTVLQFEGDRFQSFRVLRALKNRFGSTNEVGVFEMT
                     DRGMVEVEDPSAFFLSRREEDVPPGVVTVCLLEGTRPMLVEIESLVAPSPLANPRRIA
                     NGVDTGRVNMLCAVLSRRAGLAVQDQDVYVNVTGGVRVEEPAADLGVALAIASAMRDR
                     PVGKGTACFGEVGLTGDVRYVSGAPRRTSEMSKMGFGRIIRPEGAKDGPVNDGTGRGA
                     SVLEVSTLEEAVAVALL"
     gene            1262..2314
                     /gene="disA"
                     /locus_tag="AC16CM_05490"
     CDS             1262..2314
                     /gene="disA"
                     /locus_tag="AC16CM_05490"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HIR3139316.1"
                     /translation="MTRALDLVAPGTELRAAIENVIRAQNGTLIVVDNPERLETLGII
                     SGGMRIELEFTPMRLYELAKMDGAIVVSKNLSTIHYANVHLSPDPGYHSKETGTRHLA
                     ADRTAQQTGSLVIAVSERRRVVSLYQGTYGLHVLEDIGVVLSKANSAIATLEKFTLRL
                     REEARGLTVHEYDGAVTLREVVNAVVTFEYSARIAEEIETYVRELGSEGRLVEMQLEQ
                     AFHGVPEQYEALLRDYVADEPDYDSVREKLGTFTAEQLSDSEELTRALGYRPSGPLED
                     SFLKPRGYRQLERVPRLPGRVAESIIKEFGSLKERLEASEADLDEVEGVGQARARAIH
                     RGLERQRSLETTDGLS"
     gene            2375..3061
                     /gene="ispD"
                     /locus_tag="AC16CM_05495"
     CDS             2375..3061
                     /gene="ispD"
                     /locus_tag="AC16CM_05495"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3139317.1"
                     /translation="MLAGGTGSRMGRPKQFIELRGLPALQYTLQAFEDCPEVARIYAV
                     GKEPMVEDLARSVGISKYAACAEPGESRSLSTRNGLLICEEDPETIVLVHDGSRCLVT
                     PELIGRIVEAARAPSRPDGVVPGIAVSDTIKAASDGVVSKTLDRSTLWAVQTPQAFRL
                     GLLRRLHELTEEHLRTATDDASLVERDGGRVEVVAGEKTNIKLTSPEDLVLAEAILAA
                     REDATQEVRS"
     gene            3061..3540
                     /gene="ispF"
                     /locus_tag="AC16CM_05500"
     CDS             3061..3540
                     /gene="ispF"
                     /locus_tag="AC16CM_05500"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3139318.1"
                     /translation="MRVGLGIDVHAFAAPEEGRRLVLGGVEIPFDRGLLGHSDADVLA
                     HAVTDALLGAAGLEDIGHYFPDTDERFRDADSIWLLREVRALIGGTWEVSNVDAVVVC
                     ERPKIRDHRGAMRANLAVALGVDSSRVSVRGTTSEHLGFTGRGEGVAAQAVCLLEGR"
     gene            3537..4829
                     /locus_tag="AC16CM_05505"
     CDS             3537..4829
                     /locus_tag="AC16CM_05505"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3139319.1"
                     /translation="MSAVRVRFAPSPTGLLHVGGARTALFNYLFARHHGGDLVLRIED
                     TDRERSAPEFERAQVEDLAWLGLSFDEGPFRQSERLETYERAAEPLVGANLAYEKADE
                     AGRHALYFRPPERSGSFRDGLRGEVTFGAVDDFVILKSDGTPSYNFAVVVDDAEMGIT
                     HVIRGEEHLSNTARQGLLYEALDADKPRFIHLGVILGPDGKKLSKRHGAASIAEYRRE
                     GYLPEALVNYLALLGWNHPQGKEEFSGLAELADEWDPSRLGASPAVFDPDRLLFFDAA
                     HIRSLSPEELRRRLEPFLDGPLPHGRELAAVEAVREEMRTLSEATGLLREITDPVDPS
                     GFVAGLPDSSGEVFSYVAAELEGRRFDGVESARNFVAGLRAWAKERGIKARDLLHPLR
                     LALTGRNRGPEMGYLFAVLGADGARERIVRAGEARLGT"
     gene            4826..6247
                     /gene="cysS"
                     /locus_tag="AC16CM_05510"
     CDS             4826..6247
                     /gene="cysS"
                     /locus_tag="AC16CM_05510"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3139320.1"
                     /translation="MSIRVRDSLSTGTVEVGEDGRIGLYVCGPTVYDHIHIGNARAPL
                     FWDVVVRYLRSRNYDVTFVQNITDIEDKIINKANEEGIPWQEIVSRYTGSFHERLRML
                     GVRLPDVEPRATEHIPEMISLIEELIERDHAYAPEAAGGDVYYAVGSFAPYGALSHQR
                     PEEMRETEKGQTGFKRSPLDFALWKASKPGEPSWQSPWGPGRPGWHIECSAMVGKHLP
                     DGADIHGGGTDIRFPHHENELAQSTAAHPERPFVRAWAHHGMVTIGGSDKMAKSIGNV
                     LDVESAVELHGSNAIRMWLLQSHYSQPIEYSGEILEEKRRSFERLQNLYQALGGSTSS
                     SELSDRLATDLTERFDAAMQDDLNTPEAMAALFDVAGRAGREISARPGAAGEFGSLAG
                     AMEEVITVFGFDLARELSSEVEGVRIRYSKDPGEQVLERVAARETARREKDWTVADRL
                     RDELDAEGWSVEDTSEGPILRRR"
     gene            6248..6958
                     /gene="rlmB"
                     /locus_tag="AC16CM_05515"
     CDS             6248..6958
                     /gene="rlmB"
                     /locus_tag="AC16CM_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3139321.1"
                     /translation="MKREIIYGSRPVIEALESGRRRVFEVFDAVGDREVSKAAASRGI
                     TPKRVSRQRVDELARGGVHQGVVARVEPYPYSGLGEILASPDTLVLVLDGVTDPRNLG
                     AVLRAAEGAGASGVVIPRDRAAGVTAAAVKASAGASEHVRVARETNLKRAIEEMKEAG
                     LWVYAAEGNGAAYPQLDLTGPVGLVFGSEGRGVRRLVREACDGTVAIPMLGNVGSLNV
                     SVAAAVLLYEVRRQRGWP"
     gene            6949..7437
                     /locus_tag="AC16CM_05520"
     CDS             6949..7437
                     /locus_tag="AC16CM_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3139322.1"
                     /translation="MALILDGYNVIGALDRYRRAESLDAARELLINDALKASGWTGRP
                     LILVFDAHRGPGPGHAERRAGGAVRVIYSAPGESADDVIERLLGALEESATVYTADFA
                     LQRTALARGSTRATPREFATLLDELPATTHSPDVPRKSRIADRLSPETLRKLEEIRRK
                     GY"
BASE COUNT         1327 a         2341 c         2678 g         1185 t
ORIGIN      
        1 ggcagaaggc cagggccgcg gggaagacgc tcgccctgag cgaggtgccc gtcgtcgagg
       61 gcgggggcag gatggacgcc ggggtcgggg agctgaacag ggtactgggt ggtgggatcg
      121 tgcccggttc catggtgctg gtcggtggcg agcctggcgt tgggaagagc acgctcttgc
      181 tgcaggtgat ggggaatctg ggagaagggt gcctcatgat ctctggtgag gagtcgccac
      241 ggcaggtggc gttgagcgcg cgcaggctcg gggtcgaagg ccggggcttc agggtgctct
      301 cggagacgga tctggatgtg atcgaggcga ccatactgga ggagcggccg agtgccgtgg
      361 tggtcgattc catccagacg ctctactcgc cggagctctc gggagcgcct ggtggggtgg
      421 ggcaggtgcg cgagtgcgcg gcgcgcctga tgcggctcgc caagagcgag gggatagcca
      481 tggtcctcgt tggtcacgtc accaaagacg gctccattgc gggtccgcgc gtgctggagc
      541 acatggtcga caccgttttg cagttcgagg gtgacaggtt ccagtctttt cgagtcttgc
      601 gggcgctcaa gaaccgtttc ggttccacta acgaggttgg cgtcttcgag atgacggacc
      661 gcgggatggt ggaggtcgag gacccttcgg cgttcttcct ctccaggagg gaggaggacg
      721 tgccgcccgg ggtggtcacc gtgtgtttgc tggaggggac gcggccgatg ctggtggaga
      781 tagagagcct cgtcgccccg agcccgctcg ccaacccgcg gcgtattgct aacggggtag
      841 acacgggcag ggtcaacatg ctctgcgccg tgctcagccg gagggccggg ctcgcggtgc
      901 aggaccagga cgtctacgtc aacgttaccg gtggtgtgcg ggtggaagag cccgcggcgg
      961 acctcggggt ggcgctcgcc atcgcctcgg ccatgcggga taggcctgtc ggcaagggca
     1021 ccgcgtgttt cggcgaggtc gggttgacgg gggatgtgcg ctacgtctcg ggggcgccgc
     1081 gcaggacctc cgagatgtcc aagatgggct tcggccgtat aatcaggccg gagggagcca
     1141 aggacggccc ggtcaacgac gggaccggta ggggagctag cgtcttggag gtcagcacgt
     1201 tggaggaggc ggtggcggtg gcgctgctgt gagcccctcg aggaggaagc tgccaacgca
     1261 ggtgaccagg gccctcgacc tcgtggcgcc gggcacggag ctaagggcgg ccatagagaa
     1321 cgttatcagg gcgcagaacg ggaccctcat cgtggtcgac aaccccgagc ggctggagac
     1381 cctcggcatc atctcggggg ggatgaggat cgagctcgag ttcaccccca tgcgcctcta
     1441 cgagctggcc aagatggacg gcgcgatcgt ggtctccaag aacctgtcga ccattcacta
     1501 cgcaaacgtc cacctcagcc ccgacccggg ttaccactcg aaggagaccg gtacacgcca
     1561 cctcgcggcc gaccggacgg cgcagcagac cgggagcctg gtcatagccg tctcggagag
     1621 gcggagggtg gtcagcctct accagggtac ctacgggttg cacgttctgg aggacatcgg
     1681 ggtagtcctc tccaaggcca actctgccat cgccacgctg gagaaattca ccctgaggtt
     1741 gcgggaggag gcaagggggc tgaccgtgca cgagtacgac ggggccgtga cgctcaggga
     1801 ggtcgtaaac gccgtcgtta ccttcgagta ctcggccagg atcgccgagg agatagaaac
     1861 ctacgtcagg gagcttggca gcgaggggcg tctggtcgag atgcagctag agcaggcctt
     1921 ccacggtgtt cccgagcagt acgaagccct gctccgcgac tacgttgccg atgagccgga
     1981 ctacgattcc gtcagggaga agctcggaac tttcaccgcc gagcagctat ctgattctga
     2041 agagctaacc cgggccctgg gctacaggcc gtccggcccg cttgaggaca gcttcctcaa
     2101 gccaaggggg taccgtcagc tcgagcgcgt gccacgcctg cccggcaggg tggccgagag
     2161 catcatcaaa gagttcggtt ccctgaaaga gcggctggaa gcgtccgagg ccgacctgga
     2221 cgaggtcgag ggggtcggcc aggcccgcgc ccgcgccatc caccgcggcc tcgagcgcca
     2281 gcgcagcctg gagacgaccg atgggctctc ctaggggctc ggaggttccc ggggaaggcg
     2341 cgagatagag gagcaaggga cagcgatcgc gctcgtgctc gccggcggga ccggcagccg
     2401 catgggccgc cccaagcagt tcatagagct ccgcggcctg ccagccctcc agtacaccct
     2461 gcaagccttc gaggactgcc ccgaggtggc caggatctac gccgtaggca aagagccaat
     2521 ggtcgaagat cttgccaggt ctgtcggcat cagcaagtac gccgcctgcg ccgaaccggg
     2581 cgagtcgcgt tccctctcga ccagaaacgg actgctcatc tgcgaggaag accccgagac
     2641 catcgtgctc gtgcacgacg ggtctcgttg cctcgtcacg ccagagctca tcgggcgcat
     2701 cgtggaggcg gcgcgggcgc ccagccggcc cgacggcgtg gttcccggga tagccgtatc
     2761 tgacaccatc aaggccgcct ctgacggcgt tgtttcgaag acccttgaca ggtcgacact
     2821 ctgggcggta cagaccccac aggccttccg cctcgggctg ctgcgccgtc tgcacgagtt
     2881 gacggaagag cacctgcgca ccgccacgga cgacgcgtcg ctcgtcgagc gggacggcgg
     2941 cagggtcgag gtcgttgccg gcgagaagac caacatcaag ctgacctccc cggaagacct
     3001 cgtcctcgcg gaagcgatac tagccgcacg ggaggacgcc acgcaggagg tccggtcgtg
     3061 atgcgggtcg ggctcggcat cgacgtgcac gcgttcgcgg cgccggaaga gggccgcagg
     3121 ctcgtacttg gcggggtaga gatcccattt gacagaggtc tcctcggtca ttctgacgct
     3181 gacgtcctgg cccacgctgt aaccgacgcc ctgctcgggg ccgccggcct cgaagacatc
     3241 ggccactact tccccgacac ggacgagcgg tttcgggacg ccgactccat ctggttgctg
     3301 cgtgaggtac gcgctctgat cggggggacg tgggaggtct ccaacgtgga cgccgtggtg
     3361 gtctgcgagc gacccaagat ccgggaccac aggggggcca tgcgggccaa cctcgcagtc
     3421 gccctagggg tggacagctc ccgcgtgagc gtgcgcggca cgacgagcga gcacctcggc
     3481 tttacgggcc gcggcgaggg cgtggcagca caggcagtgt gcctgctaga gggccgttga
     3541 gcgcggtcag ggtacggttc gcccccagcc cgacgggctt gctccacgtc ggcggcgcgc
     3601 gcacggccct cttcaactac cttttcgcca ggcatcatgg gggggacctc gttctccgta
     3661 tcgaggatac cgacagggag cggagcgccc cggagttcga gcgggcgcaa gtggaagacc
     3721 tcgcctggct cgggctgtct ttcgacgaag gcccattccg gcagagcgag cgcctggaga
     3781 cctacgagcg tgccgcggag cctctggttg gggccaacct ggcttacgag aaagcggacg
     3841 aggcgggaag gcacgcgctg tactttcggc ccccggagcg cagcggtagc tttagagacg
     3901 ggttgcgggg ggaggtcacg ttcggggccg tggacgactt cgttatattg aagtccgacg
     3961 gcaccccttc ttacaacttc gccgtcgtgg tcgacgatgc ggagatgggg ataacgcatg
     4021 tgatccgggg cgaggagcac ctctccaaca cggcccgcca gggcctgctc tacgaggccc
     4081 tggacgcgga taagccccgg ttcatccatc tcggcgtgat cctggggcct gacggcaaga
     4141 agctaagcaa acgtcacggt gccgctagca tcgccgagta ccgtcgggaa gggtatctgc
     4201 cggaggccct ggtgaactac ctcgcgctgc tcggctggaa ccacccgcag ggaaaggagg
     4261 aattctccgg cctcgccgag ctcgcggacg aatgggaccc gtcgcgcctc ggggcgagcc
     4321 cggcggtctt cgatcccgac cgtctcctgt tctttgacgc cgcccatatc cgaagcctat
     4381 ccccggagga gcttcgtcgg cgtctggagc ctttcctcga cgggccgctg ccgcacggac
     4441 gggagctggc cgcggtggag gcagtaaggg aggagatgcg tacgctatcc gaggccacgg
     4501 ggctactgcg ggagatcacg gaccccgtcg acccttctgg cttcgttgcc gggctacccg
     4561 attcgagtgg cgaggtcttc tcctatgtcg cggcggaact tgagggccgg cgattcgatg
     4621 gtgtggagtc ggctcgaaac ttcgttgccg ggctccgtgc ctgggcgaag gagcggggga
     4681 tcaaggcccg cgacctgctc catcctctga ggctcgcgct caccggcagg aacagggggc
     4741 ccgagatggg ctacctcttc gcggtgcttg gtgccgacgg ggcgcgggag aggatagttc
     4801 gggcaggaga ggctagacta gggacatgag catacgcgta agagacagcc tgagcaccgg
     4861 cacggtcgag gtcggggagg atggtaggat cgggctctac gtctgcggtc ccaccgtata
     4921 cgatcatatc cacatcggca acgcgcgggc gcccctgttc tgggatgttg tggtcaggta
     4981 cctgaggagc cgcaactacg acgtgacgtt cgtccagaac atcacggaca tagaagacaa
     5041 gatcatcaac aaggccaacg aagagggcat cccgtggcag gagatcgtaa gccgctacac
     5101 gggctccttc cacgagcggt tgcggatgct aggcgtgcga ctaccggacg tcgagccgcg
     5161 ggccacggag cacataccgg agatgatctc cctgatcgag gaactcatcg agagggacca
     5221 tgcctacgcc ccggaagcgg ccggcgggga cgtatactac gcggttggga gcttcgcgcc
     5281 gtacggtgcc ctcagccacc agcgtcccga ggagatgcgc gagacggaga agggccagac
     5341 gggcttcaag aggagcccgc tcgacttcgc gctgtggaag gcatccaaac cgggggagcc
     5401 ctcctggcaa agcccgtggg ggcccgggcg tcccggctgg cacatcgagt gctccgccat
     5461 ggttggcaag catcttcccg acggagcgga catccacggc ggcggcacgg atatacgctt
     5521 cccgcaccac gagaacgagc tcgcccagag caccgcggcc catcccgagc gcccgttcgt
     5581 cagggcgtgg gcgcaccacg gtatggtgac catcgggggc tcggacaaga tggccaagtc
     5641 cataggcaac gtgctcgacg tcgagagcgc cgtcgagttg cacggcagca acgccatccg
     5701 gatgtggttg ctacagagcc actactcgca gcccatcgag tactcggggg agatcctgga
     5761 ggagaaacgc cgctccttcg aacgtctcca gaacctctac caggcgctcg gaggctccac
     5821 gtcgtcttcg gagctctccg accggctcgc caccgatctt acggagcggt tcgacgccgc
     5881 gatgcaggac gacctgaaca ccccggaagc catggcggcg ctcttcgacg tcgccggtcg
     5941 cgccgggcgg gagatctcgg cccgtcccgg ggccgccggc gagtttggtt cgctggccgg
     6001 ggctatggaa gaggtcatta cggtcttcgg cttcgatctg gctcgcgagc tgtcctccga
     6061 ggtggagggt gtccgcatca ggtactccaa agatcctggc gagcaagtcc tcgagcgggt
     6121 ggccgccagg gagaccgccc gccgcgaaaa ggactggacc gtcgccgacc ggcttcgcga
     6181 cgagctcgac gccgaaggat ggtccgtgga ggacacgtcc gaaggcccca tcctccgccg
     6241 ccggtaattg aagcgggaga tcatctacgg ctccaggccc gtgatcgagg cgctggagag
     6301 cggtcggcga agggtcttcg aggttttcga cgccgtggga gaccgggagg taagcaaggc
     6361 cgctgcctca cgaggtataa cgccgaagcg ggtgtcccgc cagcgggtgg acgagctagc
     6421 caggggtggc gtgcaccagg gcgtggtggc ccgtgtggag ccctatccct actccggcct
     6481 gggagagatc ctcgcctcgc ccgacactct ggtactcgtt ctcgacgggg taacagaccc
     6541 ccgcaacctc ggggccgtgc tgcgggctgc ggagggtgct ggcgcgagcg gcgtcgtcat
     6601 ccccagggac cgggccgcag gcgtaacggc cgcggctgtc aaggcgagcg cgggggcgag
     6661 tgagcacgtg cgcgtggcgc gcgagaccaa cctcaagcgt gccatcgagg agatgaaaga
     6721 ggccggcctc tgggtgtacg cagccgaggg taacggcgcc gcgtacccgc agctcgacct
     6781 caccggtccg gtggggctcg tgttcgggag cgagggacgg ggcgtaaggc ggctcgtgcg
     6841 cgaggcctgc gacggcacgg tagccatccc catgctcggg aacgtggggt ctctcaacgt
     6901 ctccgtcgca gcagcggtct tgctttacga agtgcggagg cagcgcgggt ggccctgatc
     6961 ctggacggct acaacgtgat cggtgccctc gaccgctaca ggcgagccga aagcctcgac
     7021 gccgcaagag agcttctgat taacgacgcc ctgaaagcgt cgggatggac gggaagaccc
     7081 ctgatactcg tcttcgacgc acaccgcggt cccgggccgg gccacgccga acggcgggcc
     7141 ggcggggccg tgcgcgtgat ctacagcgcc ccgggggaat ctgccgacga cgtgatagaa
     7201 cgcctcctcg gtgccctcga agagtcggca accgtctaca ccgccgactt cgcactccag
     7261 cgcaccgccc tggcccgcgg ctccacccgc gccaccccac gcgagttcgc caccctactt
     7321 gacgagctac cagccacgac acacagcccc gatgtcccgc gaaagtcccg aatagcggac
     7381 cgcctctcgc ccgaaacact gcgcaagctc gaagagatac gccgaaaggg gtattagctg
     7441 tcagccgtta gctgtcagca agtacttgct gccgaggggc gtgcaccatc gttgcccaga
     7501 accttgctgt tagaatgctg gcgacgcatc a
//
LOCUS       DBNGVD010000197         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2773608, whole genome shotgun sequence.
ACCESSION   DBNGVD010000197 DBNGVD010000000
VERSION     DBNGVD010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2773608"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16CM_05525"
     CDS             complement(<1..452)
                     /locus_tag="AC16CM_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3139323.1"
                     /translation="MRRRANLVTPVVRRERHPYARLQSTEVQLPDLVEIQLNSFNKFL
                     DEGIQKTFTEISPIEDYTGSYAVEFGEGRFEEPPVSVEECMSKDKTYAAPLFVKVRFI
                     IKETGEVREQDVFMGDFPLMTESGTFVINGTERVVVTQLVRSPGAYIM"
     gene            complement(767..1147)
                     /gene="rplL"
                     /locus_tag="AC16CM_05530"
     CDS             complement(767..1147)
                     /gene="rplL"
                     /locus_tag="AC16CM_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3139324.1"
                     /translation="MAVNKEELMETIENMTVLELSELVSALEERFGVSATAVAAAPAA
                     AAGDGAAAAEEEQTEFDVVLQGPGEKKIQVIKVVRAATGLGLKEAKALVDEAPNPVKE
                     ALSREDAEALQAQLEEAGASVELK"
     gene            complement(1174..1704)
                     /gene="rplJ"
                     /locus_tag="AC16CM_05535"
     CDS             complement(1174..1704)
                     /gene="rplJ"
                     /locus_tag="AC16CM_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3139325.1"
                     /translation="MNREEKAQVIENLTAKLRVGSAVLVDYQGMDVARSTDLRRRSRE
                     SGVDFVVAKNTLTRRAADAAGVEGLQEFLFGPTALAFSEDPVASAKLMAEFADQVESF
                     ALKGGLLEGGRVLDGDEVVALSKLPSRDQLLAQIVGGISSPLTGLVNVLNNTLQGLVV
                     ALGQIAEQKQQGAEQS"
     misc_feature    complement(1715..1853)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1878..2567)
                     /gene="rplA"
                     /locus_tag="AC16CM_05540"
     CDS             complement(1878..2567)
                     /gene="rplA"
                     /locus_tag="AC16CM_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3139326.1"
                     /translation="MGRKLLEQKSRIDPEHRYTPREALELLKSLEGAKFDESVEAHIH
                     LNVDPRRADQMVRGTLMLPNGTGKTRRVAVFAEGEKAREAEEAGADVVGSEDLASRIR
                     NDGFMDFDVAVATPDQMRSVGQLGPVLGPRGLMPNPKSGTVTQDVARAVEEIKRGKIE
                     YRVDRYGIIHGVIGKKSFDLDSLVENYFALREELQRARPAPVKGRYFKTVSLSTTMGP
                     SVKVDPAAEKD"
     gene            complement(2560..2997)
                     /gene="rplK"
                     /locus_tag="AC16CM_05545"
     CDS             complement(2560..2997)
                     /gene="rplK"
                     /locus_tag="AC16CM_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3139327.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNDATRDQMGQIIPVEITVYEDRSFTFITKTPPAAFLLKQAAGLERGSGEPNRNKVG
                     TVSRAQVEEIARTKMPDLNAADIDGASKIVEGTARSMGITVNG"
BASE COUNT          507 a         1046 c          878 g          628 t
ORIGIN      
        1 tccatgatgt aggcgccggg cgacctgacg agctgcgtca cgaccacgcg ctcggtgccg
       61 tttatgacga acgtaccgga ctctgtcatg agcgggaagt cgcccatgaa gacgtcctgc
      121 tccctgacct cgccggtctc cttgatgatg aagcgtacct tcacgaacaa cggggccgcg
      181 taggtcttgt ccttggacat gcattcttcc acggagaccg gcggctcctc gaagcgcccc
      241 tccccgaact ccacggcata agaacccgtg taatcctcta tgggagagat ctccgtgaag
      301 gtcttctgaa taccctcgtc taaaaactta ttaaatgaat tcaactggat ctcgacgaga
      361 tccggcagct gaacctcggt ggactgtaac cgagcatacg ggtgccgttc ccggcgcacg
      421 acaggggtca ccaaattagc tctcctccgc atgattcgtt aaaggatggg caaacacgca
      481 aacgaatatc ttacccccac tacaggggca ggtcaagggg tatttctgag aaatatgtgg
      541 ctgatctgga accagcgcgg agagtgtagc acagccgttc gtggcatgcc acgaacggag
      601 ctatcagcca tcagcttttg gctgtcagct tcgatctcta ccatggccac tctaaccggt
      661 cctcgaggga ttctgcacag aaacgagagc ggcgcccccg tcagggagcg ccgcctgcgt
      721 aagcccgtag ctattagctg atagctatca gctaatagct ataaagctac ttcaactcga
      781 ccgaggctcc ggcctcctcc agctgtgcct ggagcgcctc cgcgtcctcg cggctcaggg
      841 cttccttcac ggggttcggg gcctcgtcca cgagggcctt cgcctccttg aggccgaggc
      901 ctgtggcggc gcggaccacc ttgatgacct ggatcttctt ctcgccgggg ccctggagca
      961 cgacgtcgaa ctcggtctgc tcctcttcgg cggcggcggc gccgtcaccg gcagcggcgg
     1021 ccggggccgc ggcaacggcc gtggcggaga cgccgaagcg ctcctccagg gcgctcacga
     1081 gctccgaaag ctcgagcacc gtcatgttct cgatcgtctc catcaactct tctttgttta
     1141 cagccatctg tcttccttcc tgtctctact tgcctacgac tgttcggcgc cctgctgctt
     1201 ctgttcggct atctggccga gcgccacgac caggccctgc aaggtgttgt tcaagacgtt
     1261 caccagcccc gtgagcgggg aggagatgcc accaactatc tgggcgagga gctgatcccg
     1321 gctcggcagc ttgctgaggg ccacgacctc gtccccgtcg agcacgcgcc cgccttccag
     1381 caggccgccc ttcagggcga aagactcgac ctggtcggcg aactccgcca tcagcttcgc
     1441 gctcgctacc gggtcctcgg agaaggccag ggccgtcggc ccgaagagga actcctgcag
     1501 tccctcgact ccggccgcat cagcggcccg cctcgtcagc gtgttcttcg ctaccacgaa
     1561 gtccacgccg gactcgcggc tacgcctgcg gaggtccgtg ctcctggcaa cgtccatgcc
     1621 ctggtaatcg acgagcaccg cagagccgac ccgcagcttc gcggtgaggt tctcgatcac
     1681 ctgcgctttc tcttccctgt tcaccacaac tcctatctcg aatagaaaac cccgaggccg
     1741 cagaggcgct cggggtcttg tcccggagaa gctttccgca acctcggccg gatcattaag
     1801 ccctcgtctc tcgaccgggc cccggctgtc tcaggttttt gattttgtcc agggactact
     1861 tgagtccgcc ggtgatacta atccttctcc gccgcggggt caacctttac ggagggcccc
     1921 atcgtcgtgc tcagcgagac ggtcttgaag tagcggccct tcacgggggc tggacgggcc
     1981 cgctgcaact cctcgcgcag ggcgaagtag ttctctacca ggctgtccag gtcgaatgac
     2041 ttcttgccga tcacgccgtg gatgatgccg tagcggtcca cgcggtactc gatcttgccg
     2101 cgcttaatct cctcgacggc ccttgccaca tcctgcgtga ccgtgccgct cttcgggttg
     2161 ggcatgagcc cccgcgggcc gagcaccggg ccgagctggc ccacggaccg catctggtcc
     2221 ggggtcgcca cggccacgtc gaagtccata aagccgtcgt tcctgatgcg gctggcaagg
     2281 tcttcggaac cgaccacgtc ggcccctgcc tcctcggcct ccctggcctt ctcaccctcg
     2341 gcgaagaccg ccaccctacg tgtcttgccg gtgccgttag gcagcatcaa cgtgcctcgc
     2401 accatctggt cggcgcgcct ggggtccacg ttcaggtgga tgtgggcctc cacgctctca
     2461 tcgaacttgg cgccttccag gctcttgagc agctccagcg cctcacgagg cgtgtagcgg
     2521 tgttccggat ctatcctcga cttctgttcc agcagctttc tacccattga ccgtaatccc
     2581 catgctcctc gcggtacctt ccacgatctt cgacgcgcca tcgatgtccg cggcgttcag
     2641 gtcgggcatc ttggtgcgcg ctatctcctc gacctgagcc cggctcaccg tgccgacctt
     2701 gttcctgttc ggctcgccgc tgcccctctc caggccggcg gcctgcttca gcaggaacgc
     2761 ggccggcggc gtcttggtta tgaacgtgaa ggaacggtcc tcatagaccg taatctccac
     2821 ggggatgatc tggcccatct ggtcgcgcgt ggcgtcgttg aactgacgca cgaactcccc
     2881 gatgttgacc tgcgcctggc caagggccgg gccgacaggg ggcgcagggt tggcctgccc
     2941 gcccgtgatc tgcaacttca gcttgcgcgc tacgcggcgc ccgcgtcctc tacccatgaa
     3001 atcctctcta cagctttgct acctggttga aggagagctc cacgggcgtc tcacgcccg
//
LOCUS       DBNGVD010000198         6176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3320616, whole genome shotgun sequence.
ACCESSION   DBNGVD010000198 DBNGVD010000000
VERSION     DBNGVD010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3320616"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC16CM_05550"
     CDS             <1..499
                     /locus_tag="AC16CM_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3139328.1"
                     /translation="LDVADDKGVYPFVPEMIRTYLAEEPVLANIDTYSLAVEEDRRYV
                     MEHFGNLVIKSRSGWGGKDVLIAPQESREDVEQFRKQVEENPVEYVAQEMIDFSTHVL
                     CEIEDDDFVLRDAHADLRIHALAPDSESVRIVPGAMTRVAAPGNKLVNISSGGKMKDT
                     WVLSP"
     gene            complement(523..1080)
                     /locus_tag="AC16CM_05555"
     CDS             complement(523..1080)
                     /locus_tag="AC16CM_05555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3139329.1"
                     /translation="MKIAVVGRGNIGGGLANLWEKAGHEVTRIGRDGADVSDADAVLL
                     AVPGEAISEALDGLRGIGGKTFIDPTNLLGVSPPEGFPSNAEYVKSRTGGPTAKAFNT
                     NPAAVYDRLGETTVAPNNLWCGDEEAREVAERLSVDAGFEPLYGGPLDNAAEQETLAT
                     GNLILSIFEGLGEPFVYRFAPPDRF"
     gene            complement(1077..2048)
                     /locus_tag="AC16CM_05560"
     CDS             complement(1077..2048)
                     /locus_tag="AC16CM_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139330.1"
                     /translation="MGKKDEDTESGRYLSRVYLASLPERTARAGAALTGGLVYETGEV
                     LLPLVVRRSRIYQAVVGRLLRITIELVGGVDGVFPTEEMLVRELMARKTAGNVVEIGS
                     FLAVGWSPVWLLAGASDLIGGSKAYLRVLVTELEESGVLSAEEDVASFEELLSALEGT
                     SGVLADTVDVPPLNVAGVRASWQELQRQAAELPDARGLEEIFSELRLASRREGRSVME
                     ISTVVALGAVRAGVKLGNVHVFDYYRGALRTIADEGLVSFLYRTSTPYLTRARSHFDP
                     GSSTLSERLLRRWSERRSPGARRASAQDVGPSVENEAEAQERRKGAG"
     gene            complement(2149..2442)
                     /locus_tag="AC16CM_05565"
     CDS             complement(2149..2442)
                     /locus_tag="AC16CM_05565"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3139331.1"
                     /translation="MPRYVVLVNWTEQGIKNVKETIERTDRGMELAKKHGTEFKQAFW
                     TVGPYDMVAVFESPDDETLSAHLLEICASGSVRTTTMRAYDREEMSGILQRVD"
     gene            complement(2544..3995)
                     /locus_tag="AC16CM_05570"
     CDS             complement(2544..3995)
                     /locus_tag="AC16CM_05570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3139332.1"
                     /translation="MDLDGSPPTLASEVAVQRGRLDRALARASAAPLREGNRLEILRN
                     GPDTYAAWLEAISGARRWVHLDNYIFQDDEVGERFAEALMSKAGEGVRVRVLYDWFGC
                     LDVRRSFWQKMREAGVDVRAVNPPTFGGPLGMIRRDHRKLLAVDGEYASTGGVCISEG
                     WLVTSPETGLPYRDTAVGIRGPAVAEAELAFAGLWGVTGEALPEDECPDPHNITRAGG
                     AAARVVVQEPRRMRTLRMLQLLTAGVERRLWITDAYFLSMAILSQSLMSVARDGVDVR
                     VLVPATNDLPWIGALSRTGYRQFLESGVRIFEYGGPMIHAKTMVADGWWSKVGSTNLN
                     VASLVTNWEIDLVAEDTGFAGKMEELFEEDLSNAREVRLAGTDRRPRVRPERPIQTSD
                     RDARRGVVGSGSGGSATILRVGSTALQKSGYPLETHEKALAVAASSALLGVSLLSLRF
                     PRFLAWPLATTGAIYGGLGVLRAARSVLADRKP"
     gene            complement(4055..4456)
                     /gene="sufU"
                     /locus_tag="AC16CM_05575"
     CDS             complement(4055..4456)
                     /gene="sufU"
                     /locus_tag="AC16CM_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR3139333.1"
                     /translation="MQSFYKEIVLDHFQRPRNRHGLENATIEERIDNPLCGDHVTVYA
                     NVQNDTLEEVSFTGRGCSISQASASMLTEQLTGASPEDAEQKIRAFLHMMRTEENEDL
                     GELAALKGVVQTPNRTRCATLAWEAMHRALD"
     gene            complement(4534..>6176)
                     /locus_tag="AC16CM_05580"
     CDS             complement(4534..>6176)
                     /locus_tag="AC16CM_05580"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 family protein"
                     /protein_id="HIR3139334.1"
                     /translation="SAELGSSGAPGTYGTANLGDAYRIVWHYRATDEQRTFTVRYRLS
                     GLAVAYDDVVDVYWQAWGDEWQEPLGSLEASMNLPGDPRKGAVRVFGHPASVNGETSL
                     GSGSVSPRLVASDVPPEQYVEMRVVFPRELLSSTDGAKVVQGNGLQKILDQEAADARS
                     ESRALWLQRLQPVFALVFIALAVAMMAWVYMRYGREPGVDYSELYEREPPTDDPPAVV
                     SAIMGQKPSVGTREFTATLFDLIRRGVLEAQPVSVKQGGLLGEKTITDLRIDADYRDR
                     GEDYERNVAELEDFERRVLNVALRVLHNGPVNLTDFEERIEEDSEANRSSYNAFTEDL
                     KREVEGRDLVERSAGRWLGPVALLLGLFGVVWFLLSLFGLNLLAVISLAISNAFILII
                     GIAILWFVLRRILAASGSALNQLWVRRTSKGALLHARWRAFRHYLTDFSRMQEAAPAS
                     LALWEQFLVYGIALGVAEQVLDAARLQAPPEVAQGGSFYSPGYGGSVIGPTGFTFTSL
                     ENGFSSAFAPPSSSSSGGGGGFSGGGGGGGGGGGGGAW"
BASE COUNT         1030 a         2141 c         1915 g         1090 t
ORIGIN      
        1 cctcgacgtc gccgacgaca agggtgtcta tcccttcgtg cccgagatga tacggaccta
       61 cctcgccgag gagccggtgc tcgccaacat cgacacctat tctctggccg tcgaggagga
      121 caggcgctac gtgatggaac acttcgggaa tctggtcatc aagagccgca gcggctgggg
      181 tggcaaggac gtcctcatcg ccccgcaaga gtcgagggaa gacgtcgagc agttccgcaa
      241 gcaggtggag gagaacccgg tcgagtacgt ggcacaggag atgatagatt tctccaccca
      301 tgtcctgtgc gagatcgagg acgatgactt cgttctgcgc gacgcccacg ccgacctgcg
      361 catccacgcc ctcgcccctg actcggagtc ggtccggata gtgccaggcg ccatgacacg
      421 cgtcgccgcc ccgggcaaca agctcgtcaa catctcaagc ggcggcaaga tgaaggacac
      481 ctgggtactg tcaccgtaga gctttccgct ctcagccggc gctcagaagc gatccggcgg
      541 cgcgaacctg tacacgaacg gttctccgag gccctcgaag atgctcagta tgaggttgcc
      601 cgtggcgagg gtctcctgct cggcagcgtt atccagcgga ccaccgtaga gcggctcgaa
      661 gcccgcgtcg acgctcagtc gctcggcaac ctctcgcgcc tcctcatcac cgcaccacag
      721 gttgttcggc gcaacggtcg tctcgccgag ccggtcgtag accgcggccg ggttggtgtt
      781 gaacgccttc gccgtcgggc cgcccgtcct ggacttcacg tattcggcgt tggacgggaa
      841 gccctccggg ggggatacgc cgagaaggtt ggtcgggtcg atgaacgtct tccctccgat
      901 cccccgcagg ccgtctagcg cttcggagat agcctcgccg gggaccgcca gcagcacggc
      961 gtctgcgtcc gagacgtctg cgccgtcgcg gcctatcctc gtcacctcgt gtcccgcctt
     1021 ctcccagagg ttcgcgagac ccccaccgat gttgcccctg cccacgaccg cgatcttcat
     1081 cccgcccctt tcctccgttc ctgggcctcg gcctcgttct cgacgctcgg gcccacatct
     1141 tgcgccgagg ctcgtcgtgc cccgggtgaa cggcgttccg accaacgccg caagaggcgc
     1201 tccgagaggg tgctagatcc gggatcgaag tgcgagcgtg ctcgcgtcag atagggggtg
     1261 ctggttcggt acagaaagga gaccaggccc tcgtccgcga tggtccgcag cgcgccgcga
     1321 taatagtcga agacgtggac gtttccaagc ttcacgccgg ctcgtaccgc gccgagcgcc
     1381 accacagtgg atatctccat caccgaacgg ccctccctcc tggaggccaa ccgtaactcg
     1441 gagaagatct cctccaggcc cctggcgtcg ggcagctccg ccgcctgccg ctgtagctct
     1501 tgccacgagg cgcgtacgcc ggcaacgttc aacgggggta cgtccacggt gtcggcaagc
     1561 acgccggaag tgccttcgag cgcgctcagt agctcctcga aacttgccac gtcctcttcg
     1621 gccgaaagca cgccggactc ctcgagctcg gtgaccagca cccgcagata cgccttcgag
     1681 cccccgatca ggtcggaggc ccccgcaagg agccagaccg gcgaccatcc gacagccaga
     1741 aagctgccga tctcgaccac gttgccagcc gtcttgcgtg ccatcaactc tcgcaccagc
     1801 atctcttcgg ttggaaacac gccatcgaca ccgccgacca gctcgatcgt gatgcgcagc
     1861 aaccgcccta cgaccgcctg gtagatcctg gaccgtcgaa ccacgagcgg caacagcacc
     1921 tcgcccgtct cgtacaccaa cccaccggtc agcgcggccc cggcccgcgc cgtcctttcg
     1981 ggcagggagg ccagatatac ccgcgagagg taacgcccgg attccgtatc ctcgtccttc
     2041 ttgcccatca cggtcgttgt attacggcgg tgacgcgttc accccgagct ccgcgtctgc
     2101 gccaccaccc ttgctggagc ctggcgtttc ttcccgtgga aggtttgatc aatccaccct
     2161 ctgcaagata cccgacatct cttcgcggtc gtaggcgcgc atggtcgttg ttctgacgct
     2221 tccggaggca cagatctcca gaaggtgggc gctcagggtc tcgtcgtcgg gggactcgaa
     2281 aacggcgacc atatcgtagg gacccaccgt ccagaaggcc tgtttgaact cggttccatg
     2341 cttcttcgcg agctccatgc ccctgtcggt cctctctatg gtttccttga cgttctttat
     2401 cccctgctcg gtccagttca ccagcacgac gtacctgggc atatgctcct cctcttccgc
     2461 ctttgctctg ggctgtcggg tctccccgtt cgtcgtcatg atgccagagg tacaggcagg
     2521 tcacattctt tactttcacg tccctacggc ttccggtcgg cgaggaccga gcgggcggcg
     2581 cgaagcaccc cgagaccccc gtagatggcg ccggtggtgg cgagaggcca ggcgaggaag
     2641 cgcgggaagc ggaggctcag gagtgagacc ccgagcaggg cgctgctcgc cgccacggca
     2701 agggcttttt cgtgggtctc cagcgggtag ccgctcttct gtaaggccgt gctacccacg
     2761 cggaggatgg tggcggatcc gcccgatccg ctccccacga cgcccctgcg ggcgtcacgg
     2821 tcggaggtct ggatcgggcg ctccggacgc acccgcgggc gcctgtccgt ccccgccagg
     2881 cgcacctccc ttgcgttgga gaggtcttcc tcgaaaagct cctccatctt tccggcgaag
     2941 ccggtatcct ctgccaccag gtctatctcc cagttggtga cgaggctggc gacgttcagg
     3001 tttgtcgagc caaccttcga ccaccagccg tccgcgacca tggtcttggc gtggatcatg
     3061 ggtcctccgt actcgaagat cctgaccccc gattccagga actgccggta gcccgtgcgg
     3121 gagagcgccc cgatccaggg cagatcgttg gtggccggca cgagcaccct cacgtccacc
     3181 ccgtcgcggg ccaccgacat gagggactgg gagaggatcg ccatggagag aaagtaggcg
     3241 tcggtgatcc agagccgccg ctccacgccc gccgtgagca actgcagcat gcgcaacgtc
     3301 cgcatcctgc gcggctcctg gacgacgacc cgcgcggccg ccccaccggc acgcgtaatg
     3361 ttgtggggat cggggcactc gtcctcgggc aaggcctcgc ccgtgacgcc ccacaacccc
     3421 gcgaaggcca gctcggcctc ggcgacggcc ggtccacgga taccgacggc ggtatccctg
     3481 tagggaagcc ccgtctctgg gctcgtcacg agccagccct ccgagatgca cacgccgccc
     3541 gtggaggcgt actccccgtc caccgcgagc agcttcctgt gatcgcgccg aatcatccca
     3601 agcggaccac cgaaggtggg cgggttgacg gccctgacgt ccacaccggc ctcgcgcatc
     3661 ttctgccaga atgagcgccg cacgtcgagg caaccgaacc agtcgtagag cacgcggacc
     3721 ctcacaccct ccccggcctt gctcatcagg gcttcggcga acctctcacc tacctcgtcg
     3781 tcctggaaga tgtagttgtc caggtgcacc caacgccggg ccccggagat cgcctcgagc
     3841 caggccgcgt aggtgtctgg cccgttcctc aggatctcca gccggttgcc ctcccgcaaa
     3901 ggggcggcgc tcgcgcgggc gagcgcacgg tcgagccggc cgcgctgcac ggccacctcg
     3961 ctggcgaggg tgggagggct gccatccagg tccatcgaaa tcctttcggg cggtgggttg
     4021 ctggtgggcg cttcgcgggc gtccctaccg ttctctagtc cagggcgcgg tgcatggcct
     4081 cccaggccag ggtggcgcag cgggtccggt tgggggtctg gaccacccct ttcagggccg
     4141 ccagctcgcc gaggtcttcg ttctcctcag tgcgcatcat gtgcagaaac gctcgtatct
     4201 tctgctctgc atcttcgggg cttgcgccgg tcaactgttc ggttagcata gaggcggagg
     4261 cctgggagat ggagcatccg cggccggtga agctcacctc ttcgagggtg tcgttctgga
     4321 cgttggcgta gacggtgacg tgatctccgc aaagggggtt gtctatgcgc tcttcgatgg
     4381 tggcgttctc cagcccgtgg cgattgcgcg gacgctggaa gtggtctagc acgatctctt
     4441 tgtagaagct ctgcatcacc ttgcggaaga ttgtatcagg gggttatgga gcggtgttcg
     4501 tcaagggcga gccaggggcc tgtagccttc ccatcaccag gcacccccgc cccctccacc
     4561 gccaccaccc ccgcccccac cgctgaagcc gccgcctccc ccggagctgg aggaggacgg
     4621 gggagcgaag gcgctcgaga acccgttctc gaggctggtg aaggtgaaac cggttggacc
     4681 gatcacggac ccaccgtaac ccgggctgta gaagctgccg ccctgggcca cctccggggg
     4741 ggcttgcagg cgggcggcgt ccaggacctg ttcggcgacg ccgagggcta tgccgtatac
     4801 cagaaactgt tcccagaggg cgagcgaggc gggggcggct tcctgcatgc ggctgaagtc
     4861 ggtgaggtag tggcggaagg ccctccacct ggcgtggagc agggcgccct tcgacgtgcg
     4921 cctcacccag agctggttca gggccgaccc gctagcggcc aggatgcgcc gcaacacgaa
     4981 ccataagatc gcgatcccaa tgataaggat gaaggcgttt gatattgcca ggctgatcac
     5041 ggccaaaagg ttcaaaccaa agagcgagag caagaaccat accacgccga acagaccgag
     5101 caggagggct acggggccaa gccacctgcc ggcgctccgc tctacgagat cccggccctc
     5161 cacttcacgc ttgagatctt ccgtgaaagc gttgtaggag gaacgatttg cctcgctgtc
     5221 ttcctctatt cgctcctcga aatccgtcag gttgaccggg ccattgtgga gcacgcgcaa
     5281 ggccacgttc aggacccggc gctcgaagtc ctctagctcg gcgacgtttc gctcgtagtc
     5341 ctcgccgcgg tctctgtagt cggcatcgat gcggaggtcc gttatggtct tctcgcccag
     5401 gagcccaccc tgcttcaccg agaccggttg cgcttccagg acgccgcgcc ggatcagatc
     5461 gaacagggtg gcggtgaact ccctggtccc gaccgagggt ttctggccca tgatcgcgct
     5521 gaccaccgcc ggagggtcgt ccgtgggcgg ctcccgctcg tagagctccg agtagtccac
     5581 ccctggctcc cgaccgtagc gcatgtagac ccacgccatc atcgccacgg ccagggcgat
     5641 gaacaccagc gcgaagaccg gctgcaggcg ctggagccac agcgcgcggg actcggatct
     5701 ggcatccgcg gcctcctggt ccagtatctt ctgcagtccg ttcccctgca ccacctttgc
     5761 cccgtccgtc gaagatagca actccctcgg gaacaccacc cgcatctcga cgtactgctc
     5821 gggcggcacg tcgctggcga cgagcctggg ggagaccgaa ccagagccga ggctcgtctc
     5881 cccgttcacc gaagccgggt gcccgaatac cctgacggcg cctttccggg ggtctcctgg
     5941 taggttcatg ctcgcctcca gtgaacccaa cggctcctgc cactcgtcac cccaggcctg
     6001 ccagtaaacg tcgaccacat cgtcgtaggc cacggcgagg ccgcttagac gatatctgac
     6061 ggtgaacgtg cgctgctcgt ctgtggcccg gtagtgccac acgatcctgt acgcgtcgcc
     6121 caggttcgcc gtgccgtagg tgcccggcgc gccagacgac cctagctccg ccgacg
//
LOCUS       DBNGVD010000199         3251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2773925, whole genome shotgun sequence.
ACCESSION   DBNGVD010000199 DBNGVD010000000
VERSION     DBNGVD010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2773925"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16CM_05585"
     CDS             complement(<1..251)
                     /locus_tag="AC16CM_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3139335.1"
                     /translation="MEPAVSVSGLTYRYRGQQKPALGGVDLKVAEGEFVVIMGPSGAG
                     KSTLCVSLNGLIPHFFRGKMEGEVRVGGRSTREGNVGEFA"
     gene            complement(481..1491)
                     /locus_tag="AC16CM_05590"
     CDS             complement(481..1491)
                     /locus_tag="AC16CM_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR3139336.1"
                     /translation="MDFGVTLQTNPPASRVVELTRKAEDLGFSHAWTFDSHILWQEPY
                     VIHSQMLSATERITVGPLVTNPVSRDPSVTASTFATLNDMFGNRTICGIGRGDSVVRV
                     LGRKPTTLRQLEEAMHVIKELAEGRAVDYSGVGVEIPWVEDGKLEVWMAGYGPKALGL
                     IGRKADGFILQLADPVILEWTMKHVHDAAREAGRDPKEVKICVAAPAYVGDDLAHQRE
                     QCRWFGGMVGNHVADLVSRYGEDGEVPHALTDYIKAREGYDYSHHGKADNRSTDFVPD
                     GIVDRFCVLGTVEDHVKKLDELADLGVDQFNVYLMHDAMDETLEAYGEEIIPSLETRS
                     AV"
     gene            complement(1589..3118)
                     /locus_tag="AC16CM_05595"
     CDS             complement(1589..3118)
                     /locus_tag="AC16CM_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HIR3139337.1"
                     /translation="MEQEEFRDLAVNNRAELERIKTGAMSSSLYNEDLAPTGPEQRTW
                     TTYNIAALWMGMSIVITTYLLASGLMAAGMNWWQALLTISLGNVIVLIPMVLNAHAGT
                     RYGVPFPVFVRASFGVRGANFAAIARALVACGWFGIQTWLGGAALDALVTAVWDGWAS
                     VTGHTFIAFIVFWAIQLVIILTGIEGVKWFESLSAPLLLGGSIALLTWGFVAGGGVGN
                     VFSTSAELQQGNAPFWALFFPSLAANVGYWITLSLNIPDFTRYAKTQRSQVIGQALGL
                     PLTMTAFSFIGIAVTAATVVVFGEAIWDPVVLITRLTSGIPLLLILAMFIIAIAQIST
                     NMAANVVSPSFDFSNLAPRYISFRTGGVITALIGVVSFPWLILQTAGAYVFTWLVGYG
                     SLLGAIGAVMIADYWILRRRQLDLAELYKMDGKYSYSRGVNWKAIAAVFIGVIPVIPG
                     FLKAASTPDFAGVFSNPTFIESLYNYGLFFTFGVSALAYLALSMIGGRATEPAREPEA
                     T"
BASE COUNT          603 a         1013 c         1050 g          585 t
ORIGIN      
        1 gcgaactccc caacgttgcc ctcgcgggtg gagcgtcccc ccacgcgcac ctccccctcc
       61 atcttgcccc tgaagaagtg ggggatcagg ccgttcagag agacgcagag cgtgctcttc
      121 ccagccccgg aaggtcccat gatgaccacg aactccccct cggccacctt caggtcgacg
      181 cccccgagcg cgggcttctg ctgtcccctg taacggtagg taagccccga gacgctaact
      241 gccggttcca tctatgccgt acaccaaccc atgtgcgcag catatgacaa agggggcgcc
      301 cagcgctacc ccccacggta cccgggctaa atatccccac gagaatcggc gatacgatgc
      361 tcgaagttcg aataacgggt aggcgccggg atctccgcat ctagacccag gcatggttct
      421 ggatccagac gccggcgtat caacatcgag aagggagaga ggcctccgag agcaacctct
      481 ctacaccgcg gacctcgtct caagggaagg gatgatctcc tctccgtaag cctccagcgt
      541 ctcgtccatg gcgtcgtgca tgaggtagac gttgaactgg tccacgccga ggtccgctag
      601 ttcgtctagc ttctttacgt ggtcttcgac ggtgccgagc acgcagaagc ggtccacgat
      661 gccgtcgggg acgaagtcgg tggagcggtt gtccgccttg ccgtggtggc tgtagtcgta
      721 gccctcgcgc gccttgatgt agtcggtgag ggcatgcggc acctcgccgt cctcaccgta
      781 gcgggacacg aggtcggcga catggttgcc gaccattccc ccgaaccagc ggcactgttc
      841 gcgctggtgg gccaggtcat cgccgacgta ggcgggggct gccacgcaga tcttgacttc
      901 tttggggtcg cgtccggcct ccctggccgc gtcgtggacg tgcttcatgg tccactccag
      961 gatcacgggg tcggcgagct gcaggatgaa gccgtcggcc ttgcgaccga tgaggccgag
     1021 cgctttcggt ccgtagccgg ccatccacac ctcgagtttt ccgtcctcga cccagggtat
     1081 ctctactccg acgccggagt agtcgaccgc gcggccctcg gccagctcct ttatcacgtg
     1141 catggcctct tcgagttgcc tgagcgtggt cggcttgcgg cccaggaccc ttaccacgga
     1201 gtcgccgcgt ccgatgccgc agatggtgcg gtttccgaac atgtcgttga gggtggcgaa
     1261 ggtggaggcc gtcacggaag ggtcgcggct taccgggttg gtgacgaggg gcccgaccgt
     1321 gatcctctcc gtggccgaga gcatctgcga gtggatcacg tacggctcct gccagaggat
     1381 gtgcgagtcg aaggtccagg cgtgggagaa gcccagatcc tcggccttcc tggtcagttc
     1441 gacgacccgg ctggccggcg ggttggtctg gagcgtgacg ccgaagtcca tgcgttctcc
     1501 tatcttttct cgtgccgcca cccgcggagc tcggcacatc tgccgtccgc ggcgacgcga
     1561 actcgtgagc gggcgttggg catgtctctc aggtcgcctc gggctcgcgg gccggttcgg
     1621 tggcccggcc tccgatcatg gagagcgcca ggtaggccaa ggccgagacg ccgaaggtga
     1681 agaacaggcc gtagttgtac aagctctcga tgaacgtggg gttcgagaac acgccggcaa
     1741 agtcgggggt ggacgctgcc tttagaaagc cggggatgac cggtatcacg ccgatgaaca
     1801 ccgcggcgat ggccttccag ttcacgccgc gcgagtaaga gtacttgccg tccattttgt
     1861 agagctcggc caggtccagt tgcctgcggc gtaagatcca gtagtccgcg atcataaccg
     1921 ccccgatggc cccgaggagg ctgccgtagc cgacgagcca ggtaaaaacg taggcgccgg
     1981 cggtctgtag gatcaaccag ggaaaagaaa ccacgccgat gagggcggtg atgacgccgc
     2041 ctgtcctgaa cgagatatac ctgggcgcca ggttcgagaa gtcgaacgag ggcgagacca
     2101 cgttcgcggc catgttcgtg gatatctggg ctatcgcgat tatgaacatg gcgaggatga
     2161 ggagaagcgg gatgccgctc gtgagcctcg tgatcagaac gaccgggtcc cagatggcct
     2221 ccccgaagac gacgacggtc gccgccgtga ccgcgatccc gataaacgag aaggccgtca
     2281 tggtaagcgg gagcccgaga gcctgcccga taacctggga gcgttgggtc ttcgcgtagc
     2341 gggtgaagtc cgggatgttg agggagagcg ttatccagta ccccacgttc gccgccaggg
     2401 acgggaagaa cagcgcccag aacggggcgt tgccctgttg gagttcggcc gaggtcgaga
     2461 acacgttgcc cacgccgccg cccgcaacga acccccaggt caggagcgcg atgctgccgc
     2521 cgagaaggag cggtgcggag agagactcga accacttcac gccctcgatg ccggtcagga
     2581 tgatgacgag ctgtatagcc cagaacacga tgaaagctat aaacgtgtgc ccggtgacgc
     2641 tcgcccagcc gtcccagacc gccgtgacga gagcgtcgag ggccgccccc cctagccacg
     2701 tctggatgcc gaaccagccg caggccacca gcgcccgggc tattgccgca aagttggccc
     2761 cgcgcacgcc gaaggaggca cgcacgaaca cgggaaaagg aacgccgtac ctggtgcccg
     2821 cgtgggcgtt gaggaccatg gggatcagga caatgacgtt acccagggag atggtcaaga
     2881 gtgcctgcca ccagttcatt ccggcggcca tgaggcccga ggccaaaagg tacgtggtga
     2941 tgactatgga catccccatc cagagcgccg cgatgttgta ggtcgtccac gtccgctgct
     3001 ccggtccggt cggggccaga tcttcgttgt acaggctgga cgacatggcc ccggtcttga
     3061 tgcgctccaa ctctgcccgg ttgtttaccg caagatcccg gaattcttcc tgctccatga
     3121 tcctcccttc ggtcgattca aggcttcagg ctgcaggttt caagcatcag gtagagtttc
     3181 ctgaagcctg ttgccttatg cctgtagcct atttcgccca ctccatggtc ttgatgacga
     3241 ggttgtgcag c
//
LOCUS       DBNGVD010000200         4412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_953170, whole genome shotgun sequence.
ACCESSION   DBNGVD010000200 DBNGVD010000000
VERSION     DBNGVD010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_953170"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1448
                     /locus_tag="AC16CM_05600"
     CDS             267..1448
                     /locus_tag="AC16CM_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR3139338.1"
                     /translation="MRRVRRRGSDGVSQRASSLELFYDLVFVFAITQVSHLLLTHLTW
                     AGVLQAAIVLLAVYWSWNYTTWTTNELDTETVPVRLLLLGLMLVSLLMSVAIPKAFGD
                     QALLFAGSYVAIQVGRHSFLAFAAADSGTVERERAGRILTWFVAVGVLWIAGAFVDGT
                     GRYVLWLVALSLDYCAPFVTYWVPGRRRLAPETWNVGTEHFAERFGLFIILTLGESIV
                     ITGATTSEGVLNWKTILAFVMAFLASAAIWWLYFTSVAALGEHYLEVADHRTTLARDA
                     YTVLHVVFVAGIILSAVGDELVIAHPTDVLPPYEVAAVAAGPAVYLLAHSLFTRRLTG
                     KWYPSKLLGTLACVAVGFVGLFVPALVLAAALILVLVAVIAAGYLAPRSRVEGAELRQ
                     G"
     gene            1536..2774
                     /locus_tag="AC16CM_05605"
     CDS             1536..2774
                     /locus_tag="AC16CM_05605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3139339.1"
                     /translation="MKTIIVGAGLAGLTCAKVLRERGVEVAIYEASDGVGGRVRTDEQ
                     DGFLLDRGFQVYFTSYPVSRRHLDYGALDFRVFDPGAVVCEGGEKSVLSDPLRDPKAL
                     VPSLLSRAATFGDKLKTLDLAVRTVQGGISAGSENGEPDTSTLEYLRTAGLSQQYIDS
                     FFRPFYGGIFLNRELSTSARVLRFTLRMLSTGRTVVPALGMAEIPRQLASRLPEDAVR
                     LESPADSLLRDGDRVVGITSGGREHEADAVVVATDAPAAGELTGEAVPEGAVGEACIY
                     YETDGIGEAKKILLNAGEAPFVNNAVQISNVSERYAPPGRHLLSAVALTGLDLPDGEI
                     YRRGIEDVSSWYPDTDFRPLAIYRIPYGQFAQPPGVHGRLPENRTQTPGLFLAGEYTE
                     DASINGSMLSGEKAAGAVLA"
     gene            2771..3523
                     /locus_tag="AC16CM_05610"
     CDS             2771..3523
                     /locus_tag="AC16CM_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139340.1"
                     /translation="MSDRNGVPALRIQDLRKTYSNGFLALDGVSLEVEAGKFFGLLGP
                     NGAGKTTLINSVVSLVRPDAGSVEVFGRDAYSEFREARRIIGVSPQEVNLDKFLTVQE
                     TLVFHAGYYGVPKKKARERAEELLERFGLTEKRDQRTNTLSGGMKRRVLFARALIHDP
                     KVLFLDEPTAGVDVELRYRLWGYIRELNRGGMTILLTTHYLEEAEALCDEIALINGGN
                     IVARGSSEDLKSDYEARNVEEVYMKVIGHDLS"
     gene            3510..4277
                     /locus_tag="AC16CM_05615"
     CDS             3510..4277
                     /locus_tag="AC16CM_05615"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139341.1"
                     /translation="MILADRTFQTLLTREVLRFTRVWMQTIVPQILTSLLYLVVFGIA
                     LGGRIKQIEGIPYLDYILPGIALMSLITNSQMNSSWSVFDAKRERYIEEVLISPMSNL
                     QIALAYSLGGMLRGVVMGAGVFLVGIPFVGISIKHPLMLVVIGLLASFAFSSLGTMVG
                     ALANRIEHISLITSVIIQPLAFLGGVFYSVGMLPPLLRVATFFNPIFYTVDAARYVTL
                     GVSDLNPYPTLGMVCLIAVICFGGAWWAISRGPNLRY"
BASE COUNT          719 a         1410 c         1433 g          850 t
ORIGIN      
        1 cgctcgtcga tcccgagcaa cctcggcgcc cagaggaagc cgaccctttc gaggtagcgc
       61 aggagttcgt gcacgaaggg ggcgttcggt cccatgggcc tacggaccgt gtccccgacg
      121 cgtacgatgc caggtgtcga ccggccgccg acgagcggag tttcgccgcc ttctctcacg
      181 gacatatcct atccgcccac gcgttctcgg ggagatgctc ccagggtggt tgggcggcgg
      241 ttaaccgatg cggatacact tcatccatga ggcgtgtgag gcggcggggg tctgacgggg
      301 tcagccagag ggcctcgtct ctggagctct tctacgacct cgtgttcgtc ttcgccatca
      361 cgcaggtctc ccacctgctc ttgacgcacc tgacgtgggc aggggtattg caggccgcga
      421 tcgtgctgct cgcggtgtac tggtcctgga actacacgac gtggaccacc aacgagcttg
      481 acacggagac cgtgccggtc cgactgctcc tcctcggtct catgctcgtg agcctgctca
      541 tgtcggtggc catcccgaag gccttcgggg atcaggcgct gctcttcgcc ggaagttacg
      601 tcgctataca ggtcgggagg cactccttcc tggcgttcgc ggcggccgac agcgggacgg
      661 tagagcggga gcgggccggg cgcatcctca cctggttcgt ggcggtcggc gtactctgga
      721 tcgcgggcgc cttcgtggat ggtacaggcc gctacgtgct ctggctggta gcactctcgc
      781 tcgactactg cgccccgttc gtgacgtact gggtgccggg caggcggcgg cttgcgcccg
      841 agacctggaa cgtggggacc gagcacttcg ccgagcgttt tggcctgttc atcatcctta
      901 cccttggcga gtcgatcgtc ataaccggtg cgaccacctc agaaggcgtc cttaattgga
      961 agacgatcct ggcgttcgtc atggccttcc tggccagcgc ggccatctgg tggttgtact
     1021 tcacgtcggt tgcggcgctc ggcgaacact acctggaggt cgccgatcac aggaccacgc
     1081 tcgccaggga tgcctatacg gtcctgcacg tggtgttcgt ggcaggcatc atactatccg
     1141 cggttgggga cgagctggtg atcgcgcacc cgaccgacgt actgcccccc tacgaggtgg
     1201 ccgcggtggc cgccggcccg gccgtgtacc tgctggccca ctcgctcttc acccgcaggt
     1261 tgacgggcaa atggtaccct tccaagctcc tcgggacgct cgcctgcgtc gcggtcggct
     1321 tcgtcggcct cttcgtgccg gcgctggtgc tggcggcggc cctcatcctg gtactcgttg
     1381 cagtgatagc cgccggctat ctcgcgccga ggtcccgggt ggagggcgcg gaactccgtc
     1441 aggggtgacc tggcacacca tttctcgcta aaggcccctg ccggcgtcaa aggtgttact
     1501 tgatcttcgg gattgtgcgt taaactctcg cgggtatgaa aaccatcatc gtcggggccg
     1561 gtcttgccgg cctcacctgc gcaaaagtgt tgagagaacg aggtgtcgag gtcgccatct
     1621 acgaggcttc cgatggcgtc ggtggtcgcg tgcgcaccga tgagcaagac gggtttctgc
     1681 tcgacagggg cttccaggtg tacttcacct cctatcccgt ctcacgcagg cacctcgact
     1741 acggggcgct agatttcagg gtgttcgatc ccggtgcggt cgtctgcgag ggaggcgaga
     1801 agagcgtgct ctccgacccc ctgcgcgacc cgaaggcgct cgttccctcc ctgctctcca
     1861 gggcggcaac gttcggggac aagctgaaaa ccctggatct ggcggtgaga accgtacagg
     1921 ggggcatcag cgcgggctcg gagaacggcg aaccggacac ctctacgctg gagtacctgc
     1981 ggaccgccgg cctgtcacag cagtatatag actcgttctt caggccgttc tacggcggca
     2041 tattcctgaa ccgcgagctg agcacgagcg cccgcgtact ccgtttcacc ttgaggatgc
     2101 tgtctacggg ccgcacggtc gtgcctgccc tcggcatggc cgagataccg cgccagctcg
     2161 cctcccgtct accggaagac gcggtccgac tcgaaagccc cgcagactcg ctgttgcgcg
     2221 acggagatcg ggtggtcggc atcacctctg gcggacggga gcacgaggcc gacgccgtcg
     2281 tcgtggccac cgacgcgccc gcggcggggg agctcacggg cgaggctgtt cccgaggggg
     2341 ccgtcggcga ggcgtgcatc tactacgaga cggacgggat cggggaagcg aagaagatcc
     2401 tcctgaacgc cggtgaagca ccgttcgtga acaatgccgt ccagattagc aacgtctccg
     2461 agaggtacgc gccgcccggc cgccaccttc tctccgccgt ggcgctcacc ggcctggatc
     2521 ttccggacgg ggagatctac cgccggggca tagaggatgt gagcagctgg taccccgaca
     2581 cggacttcag gccgctcgcg atctaccgca taccgtacgg gcagtttgcc cagcctcccg
     2641 gggttcatgg caggctgccg gagaacagaa cgcagacgcc aggactcttc ctggctggcg
     2701 agtataccga ggacgcctcc ataaacggct ccatgctctc tggagagaag gccgctgggg
     2761 cggtgctcgc gtgagcgacc gcaacggtgt tccggcgctc cggatccagg acctcaggaa
     2821 aacatattcc aacgggtttc tggcgctcga tggtgtctcg ctggaggtgg aagccggcaa
     2881 gttcttcggt cttctcgggc ccaacggcgc gggcaagaca accctgatca acagcgtggt
     2941 cagcctcgtc aggcccgacg ccggctcggt ggaggtcttc ggcagggatg cctacagcga
     3001 gttcagggag gcgcggcgga tcatcggggt ctccccccag gaggtcaacc tggacaagtt
     3061 cttgaccgtg caggagacgc tcgtcttcca cgccggctac tacggggtgc cgaagaagaa
     3121 ggcccgcgag agggccgagg agcttctgga gcggttcggg ctcacggaga agcgcgacca
     3181 gcgcaccaac acgctctccg gcggcatgaa gcggcgggtg ctctttgcca gggccctcat
     3241 acacgacccc aaggtcctct ttctggatga gcccacggcg ggcgttgacg tggagctacg
     3301 ctacaggttg tggggctaca tcagggagct aaaccgtggg gggatgacca tactcctcac
     3361 gacgcattac ctggaggagg ccgaggcgct atgcgatgag atagcgctta tcaacggcgg
     3421 caacatcgtc gcccggggca gcagcgagga tctcaagagc gattacgaag ccaggaacgt
     3481 cgaggaagtc tacatgaagg tgatcggcca tgatcttagc tgacaggacc ttccagacgc
     3541 tgctcacgcg cgaggtcctg cgcttcactc gtgtctggat gcagaccata gtgccgcaga
     3601 tcctgacctc tttgctctac ctggtggtct tcggcatcgc gctcggagga cgcataaagc
     3661 agatagaggg catcccctac ctggactaca tcctgcctgg catcgccttg atgagcctca
     3721 ttaccaactc gcagatgaac tcatcgtggt cggtcttcga cgccaagcgc gagcgctaca
     3781 tagaagaggt cctgatcagc cccatgagca acctgcagat agcgctggcc tacagcctcg
     3841 gcggtatgct gcgcggcgtg gtgatggggg ccggggtgtt cctggtcggg atacccttcg
     3901 tggggataag catcaagcac ccgctcatgc tcgtggtcat aggtcttctg gcctcgttcg
     3961 cgttctcctc cctcgggacg atggtcgggg cgctggcgaa ccgcatagag cacatctccc
     4021 tcataacgag cgtcatcatc cagccgctgg cgtttctggg cggcgtgttc tactccgtcg
     4081 gtatgctgcc ccccctgctc cgggtggcca cgttcttcaa ccccatcttc tacaccgtcg
     4141 atgcggcccg ctacgtgacg ctgggcgtct ccgatctgaa cccctacccg accctgggta
     4201 tggtctgcct gatcgccgtc atctgcttcg gcggcgcctg gtgggccata agccgtggcc
     4261 cgaacctccg gtactaggtt cactccttcc gcccgggcgt gggcttacgt tctcgccgtt
     4321 ctcgtctcat cgcgtttgct gttgatgggg gccggcgccc tggctgtggc ctttcttccc
     4381 gacgccgacc cggcggggga ccccctggag cc
//
LOCUS       DBNGVD010000201         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4232416, whole genome shotgun sequence.
ACCESSION   DBNGVD010000201 DBNGVD010000000
VERSION     DBNGVD010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4232416"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1067
                     /gene="cbiB"
                     /locus_tag="AC16CM_05620"
     CDS             159..1067
                     /gene="cbiB"
                     /locus_tag="AC16CM_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR3139342.1"
                     /translation="MSGLLLDAAFGEPPEAIHPTVLMGRAISMFEGSAFVGEEPESRR
                     LAGIVLALSLPALVFISTTRLLDALPERLRGLVGAILISTTLSMRGLAEAAGGVERAL
                     AQRRLEEARTRVGRFVGRDTDGLSEPEVCRAAVESVAENASDGVVAPMLYGVLFGAPG
                     ALAYKAINTLDSMVGHPQPPHAELGWASARLDDLANLAPSRATAIAVAIVSGRPLRTL
                     RIARRYGPLTASPNAGVAEAAFAGALGVVLGGTNSYGGVPRSGPTLGEGRPPEAGDIP
                     RAVAIMRRCCVLLGAIALLKAAAARG"
     gene            1111..2127
                     /locus_tag="AC16CM_05625"
     CDS             1111..2127
                     /locus_tag="AC16CM_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3139343.1"
                     /translation="MAAVSRDLQVSVEALLDFSQNINPLGPPPQALDAARRALFEESG
                     RYPDLEYRELREGLAGYLEVPPENVVPTNGGAEALFLAAGAARAGDALILEPTFSEYA
                     AAARASGRGVVRRLVRRPEDGFRFDHAALGDLRGISVVFLCNPNNPTGESLDPAEVLG
                     IADRVREAGALLVVDEAFADFAPGISVGGMVGESLWVARSFTKFFAIPGLRLGCLICD
                     DAELVKRLQPSWPVNAVAAAAGIAAVDDAGFSTASVSEVTRLRDDLYFALDNLPGLQP
                     YPGAANFLMVRGPEALAGRLARRGLLIRGCEPFMGLGAGFFRVAVRGEAENERLLSAL
                     REEL"
     gene            2142..2627
                     /locus_tag="AC16CM_05630"
     CDS             2142..2627
                     /locus_tag="AC16CM_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR3139344.1"
                     /translation="MLGGARSGKSALAERLASEIGGDSVLYVATAIVGDDEDLRRRVR
                     FHRDRRPTEWGTLEVGNLDDVVDAAEGWDAVLVDSLTLWVSSREADQALPEFEGFLAG
                     VDGLEVPFVLVSDEVGLGVVPESAAGRTFRDQLGLVNQRAAAAAREVHLCVAGVDLRI
                     R"
     gene            2665..>3232
                     /locus_tag="AC16CM_05635"
     CDS             2665..>3232
                     /locus_tag="AC16CM_05635"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR3139345.1"
                     /translation="MRGASLDEAAREVHLLPLVGLVTGLPGALVLSSALFPPGVASTI
                     ALGAALLLAGLHHADGVLDVGDALMARGNVERRRAVLKDARVGMGGLGALFLVYAPSV
                     AALTALSIESPVRVALALLAGEVAARSAMVLVLSYGEPAEAASSTVPFVRALTGPRHA
                     LATVLALLAPLPFLFALEALAPLAVLAAP"
BASE COUNT          428 a         1070 c         1144 g          590 t
ORIGIN      
        1 ctcggcagga cgttggcgaa cttcgttttg cgcgccgttc tgctcggcac tgtcggcggt
       61 ctggtcgggg cgatccgcgg tcgaccctcc tccatgagga acgagaagcc cgaagtcgcg
      121 ccacgcacgg aatgagttcc tggcatggcg gacaggtcgt gtctggtctg ttgctcgacg
      181 ctgcgttcgg agaacccccc gaagccatcc acccgaccgt gctcatggga cgcgctatct
      241 cgatgttcga aggctcggct ttcgtggggg aagagccaga atcgagacgt ctcgccggca
      301 tcgtcctcgc cctctcgttg cctgcgctgg ttttcatctc gaccaccagg ctcctcgatg
      361 ccttacccga aaggctgcgg ggtctggtag gggccatcct tatctccacc acgctctcga
      421 tgcggggcct ggccgaagcc gcgggtggcg tcgagcgagc tctggcgcaa cgaaggctcg
      481 aagaagcaag aacgcgggtg ggacgcttcg tcggtcggga cacggacggg ctctcggagc
      541 ccgaggtttg ccgggccgcc gttgaatcgg tcgccgagaa cgcgagcgat ggcgtggtcg
      601 cgccgatgct ctacggcgtc ctcttcgggg ccccgggcgc gctcgcgtac aaggccatca
      661 acacccttga ctcgatggtc ggacaccccc agcccccgca cgcggagctc ggctgggctt
      721 ccgcaaggct tgacgatctc gccaacctgg cgccttcccg ggcaaccgcc atcgccgtcg
      781 cgatcgtctc gggaaggccg ctgcgtacgt tgcggatcgc ccgccgctac ggtccgctca
      841 ccgccagccc caacgcgggc gtcgccgagg ccgcgttcgc tggtgccctc ggcgtggtac
      901 tcggtggcac gaacagttac ggtggcgtgc cgaggagcgg tcccacgctt ggcgagggcc
      961 gtcctccgga ggccggcgac atcccccgcg cggtggccat catgcgccgc tgttgcgtgt
     1021 tgctcggcgc catagccctt ctgaaagcgg cggcagcgcg tggttgaggg ctggtccgat
     1081 cacgcggggg accgccacca tggggctcac gtggcggccg tgtcgaggga tctgcaggta
     1141 agcgtcgaag ctctcctcga tttcagccag aacataaacc ctcttggccc accaccgcag
     1201 gcgttggacg ccgcgcgaag ggccctcttc gaagagtccg gccggtaccc cgatctggag
     1261 tacagagagc tgcgcgaggg cctcgccggg tacctggagg ttcctcccga gaacgtcgtc
     1321 ccgaccaacg gcggggccga agcgctgttc ctcgccgctg gggcggccag ggcgggggat
     1381 gccctgatcc tggagcccac gttctcggag tacgcggcgg cggcgcgggc ctcgggcagg
     1441 ggggtcgtcc gaaggctggt gagaaggccg gaagacggct tccgctttga ccacgccgcg
     1501 ctcggagacc tccggggcat ctctgtagtg tttctctgca acccgaacaa ccccacgggt
     1561 gaatcgctag accccgccga ggttctcggg atagcagatc gcgtcaggga ggccggggct
     1621 ctcctcgtcg tcgacgaggc cttcgcggac ttcgcaccgg gtatcagcgt cggcgggatg
     1681 gtgggggaga gcctgtgggt cgcgcggtca ttcaccaagt tctttgcgat ccccggtctc
     1741 cggctcggtt gcctgatctg cgacgatgcg gaactcgtca agagattaca gccctcgtgg
     1801 ccggtgaacg ccgtcgccgc cgccgcaggc atcgccgctg tcgacgacgc aggcttctct
     1861 acggcctcgg tgtcagaggt cacgcgcctg cgagacgacc tctacttcgc cctggacaac
     1921 cttcccggct tgcagcctta ccccggcgcg gccaactttc tgatggtacg ggggcccgag
     1981 gcgctcgccg ggcggctcgc gcgccgcggc ctgctcatcc ggggctgcga gccgtttatg
     2041 ggtctgggtg ccgggttttt ccgcgtcgcc gtaaggggcg aagccgagaa cgagcggctg
     2101 ctctcggccc tgagggagga gttgtagccg tccacgccct catcctcggc ggcgcccgga
     2161 gcggcaagag cgccctcgcc gagcgtctgg cctccgagat cgggggggac agcgttcttt
     2221 acgtggcaac ggctatcgtt ggagatgatg aagacctgcg ccggcgcgtg cgatttcaca
     2281 gggaccgcag gcccaccgaa tggggcaccc tcgaggtcgg gaacctcgac gacgtcgtgg
     2341 acgcggcgga gggttgggat gccgtgcttg tcgattcgtt gaccctgtgg gtgtcgtcgc
     2401 gtgaagcgga ccaggcgctc cccgagttcg aaggcttctt ggccggtgtc gatggcctcg
     2461 aggttccttt cgtgctggtc agcgatgagg tcgggctggg ggtagtccca gaatctgcgg
     2521 cggggaggac cttcagagac cagctggggc tcgtcaacca gcgggccgcc gcggcggcgc
     2581 gggaggtcca cctctgcgtc gccggggtgg acttgaggat caggtagctt gggagccctg
     2641 ctgtcctttt ttacgatcct gcccatgcgt ggggcgtctc tggatgaggc ggccagggag
     2701 gttcacctct tgccgctcgt cggcctcgtc acggggttgc ccggggccct cgtgctgtcg
     2761 tcggcgctct tcccgcccgg ggtggcatcc acgatcgcgc tcggggccgc cctgctcctg
     2821 gctggcctgc atcacgccga cggcgtactc gacgtggggg acgcgctgat ggccaggggt
     2881 aacgtggaga ggcggcgggc ggttctcaaa gacgcccgtg tcggcatggg cggcctcggc
     2941 gcgcttttcc tcgtctacgc cccgagcgtg gcggccctta cggcgctgtc gatcgagtcg
     3001 cccgtccgag tggcgttggc cctgctggcc ggtgaggtgg cggcccggtc ggcgatggtg
     3061 cttgtactct cctatggtga gccggcggag gcggcgtcct ctaccgttcc cttcgtcagg
     3121 gcgcttacag gaccccgcca cgcccttgcc acggtgctgg cgctgctggc gccgctgccg
     3181 tttctctttg cgttggaggc cctcgcacct ctcgccgtgc tcgccgcacc tc
//
LOCUS       DBNGVD010000202         6345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_588483, whole genome shotgun sequence.
ACCESSION   DBNGVD010000202 DBNGVD010000000
VERSION     DBNGVD010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_588483"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16CM_05640"
     CDS             complement(<1..719)
                     /locus_tag="AC16CM_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3139346.1"
                     /translation="MILDFERPIKDFEDRITELRRLAGPSEELRGEISRLEEALDAAR
                     RRIYSNLTPYQRVQVARHPERPNFRAYRDALADDFYELSGDRVFGDDPAVRGGLARIG
                     DHNVVLLGHDKGTDVNSRVKGNFGMAHPEGYRKVKRFYERAARFGLPVVVLVETPGAF
                     AGRGAEERGQAWAISDDLMTLARIPVPVISVIIGEGCSGGALAMCATDYVGMLENSYL
                     SVIAPEACASIIFRDPSRAAEA"
     gene            complement(716..1558)
                     /gene="accD"
                     /locus_tag="AC16CM_05645"
     CDS             complement(716..1558)
                     /gene="accD"
                     /locus_tag="AC16CM_05645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3139347.1"
                     /translation="MRNWLSKRREYSRTVPETESGVGPMDSVFTKCEKCGEPIYEKDL
                     RARFNVCPHCEFHYPLSASERIRLLTDSGSFEERDAGILADDLLGFENYRQRLQKARG
                     RTGLDDAILSGTGAVGGKRVALAVLDFSFIGGSMGSVVGERVARTVELAGSEELPLVI
                     VSASGGARMFEGVYLLMQMAKTSVALSRYVEGSWPYISVLTDPTFGGVTASFATAADV
                     VLAEPGARIGFTGARVIEQTTKERLPEGFQTAEFQKEHGMVDRIVHRLSLKADLERLL
                     GFLP"
     gene            complement(1555..2349)
                     /gene="trpA"
                     /locus_tag="AC16CM_05650"
     CDS             complement(1555..2349)
                     /gene="trpA"
                     /locus_tag="AC16CM_05650"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3139348.1"
                     /translation="MSGEHVRAAFREGGTALVPYLTGGYPSLEGAREVGEAFLEAGGD
                     VLEIGVPFSDPLADGPTVQNTTTLALQNGADPDYCLDLASGFSGRVPVVFLLYYNVIF
                     ARGPERFLREAADAGVSGLVIPDLPVDEAGHFAALADEAGIAFCPLAAPTSTDERISR
                     VGEMATGFVYCVSVAGVTGVRDTLPPGAVELLRRVRGRTEAPVALGFGIGSAEAAVEA
                     AGEADGVIIGSKLMRLVEEGGAGHAGTWLRGVREALSGVTKTGRRA"
     gene            complement(2346..3605)
                     /gene="trpB"
                     /locus_tag="AC16CM_05655"
     CDS             complement(2346..3605)
                     /gene="trpB"
                     /locus_tag="AC16CM_05655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3139349.1"
                     /translation="MRQETDKGYFGPFGGRYVPETLIHALEELEEAYERYRNDPEFEE
                     DRDRLARDFVGRPTPLMFAERLTRKWGGANVWFKREDLAHTGAHKINNTLGQILLADR
                     MGKERIIAETGAGQHGVATATVAALYGKDCVVYMGEEDTRRQRLNVVRMKLLGAEVVP
                     VLSGSRTLKDAVNEAIRDWVTNVEDTHYIIGSVVGPAPYPRIVRDFQAVIGSEIENQC
                     RERLGSDPDAIVACVGAGSNAIGAFHPFVGRKGVRLVGVEAAGRGLSSGEHGASLAEG
                     NLGILHGAMSYVLQTDAGQIREAHSISAGLDYPATGPEHAFLKDEGLAEYASVTDKEA
                     LEALQECSRAEGIIPALETSHALYYAETVARELGPGKNLVVNISGRGDKDVEVAANAL
                     GIDAEEAEAVSDSEAPSTTSETDEAAF"
     gene            complement(3589..4203)
                     /locus_tag="AC16CM_05660"
     CDS             complement(3589..4203)
                     /locus_tag="AC16CM_05660"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3139350.1"
                     /translation="MTFVKICGITNAGDARLAAEAGADAVGFIFAGSPRHVGIEEARS
                     ISVALPEDTIRVGVFVDEEPAKILRISQEVGLDLVQLHGDETPETVTDLREAGVKVMK
                     ALRVRSAATLEAIDEYEADLFLLDAYSARARGGTGERFDWGVAKSLRGRDNIVVSGGL
                     GPENVREAVEFFKPFGVDASSSLEDGPGRKNGELVRRFVLAARN"
     gene            4389..5744
                     /gene="ggh"
                     /locus_tag="AC16CM_05665"
     CDS             4389..5744
                     /gene="ggh"
                     /locus_tag="AC16CM_05665"
                     /EC_number="3.2.1.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004573 - Glc3Man9GlcNAc2 oligosaccharide
                     glucosidase activity [Evidence IEA];
                     GO_function: GO:0102547 - glucosylglycerate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate hydrolase"
                     /protein_id="HIR3139351.1"
                     /translation="MRPEDIVARAATVLRENDMGGWTRASPTLYPHQWSWDSAFIAIG
                     LAHLDTGRAAQEIGSLLDHQWDNGKIPHIVFNPHAPPQSYFPGPEHWASAADAPDAPT
                     GPATTSGLCQPPVHAIAALRILRVAEARGEGVEEARAFLGEVYTKLLAWHRYLATSRD
                     PEGSGLVTIFHPWESGTDNSPRWTPALERVEIGKIPYYERLDLGHVDPSERPTGYEYD
                     RFIWLVDIIKGADCDDEVLFTKCPFMAKDVLASAILVRANEALVEISDCVGAPEEDRA
                     TLRAWIERGRKGLDARWNPDLGLCSDYDLRAGETLPARTIAGFAPLIAGSEREDQLEA
                     LMLTLDSPLFAGDEDLRWPLPPSTSPQEKGFLPRSYWRGPVWPVINWLLWWSLDGAGE
                     RKRADRLKAYSLAQLSDGCFGEYYEPFTGEALGSDDQSWTAAVALDWLSDEELNGSLA
                     R"
     gene            5741..>6345
                     /locus_tag="AC16CM_05670"
     CDS             5741..>6345
                     /locus_tag="AC16CM_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3139352.1"
                     /translation="MTGLRVGDAFPDFRLPDQDGRRRRLSDYTRPGPLDERLGFDDGY
                     PLVLVFGRGFFCPRDQEQMRGLVRFQSELAVNYCKLISVSADSQRVSAAFRAGLGARW
                     PFLSDEGREVIRSIDILDETEGEYAGVSRPFTFVLAPGLEIHRIYDGWFFVGRPTLEE
                     LRQDLREIMRTRKDYRYEAYDNPGVKRIRIPQQEWADGPPPL"
BASE COUNT         1072 a         2177 c         2029 g         1067 t
ORIGIN      
        1 gcctcggcgg ccctgctcgg gtccctgaag attatggagg cgcaggcctc gggcgcaatg
       61 acggagaggt aggagttctc aagcatcccg acatagtcgg tcgcgcacat ggcaagggcg
      121 ccgcctgagc aaccctcgcc gatgataacg gagattacgg gcactggaat cctcgcgagc
      181 gtcatgaggt cgtcggagat ggcccaggcc tgtccccgct cctcggcgcc cctgccggcg
      241 aaggcgccgg gtgtctcgac gagcacgacc acggggagac cgaacctggc ggcccgctcg
      301 tagaaccgct tcaccttgcg gtatccctcg gggtgtgcca tgccgaagtt acccttcacc
      361 ctgctgttga cgtccgttcc cttgtcgtgg ccgaggagca cgacgttgtg gtcgcctatc
      421 cgggccagcc cgccccgcac ggccgggtca tcgccgaaga cgcggtcgcc ggagagctcg
      481 tagaagtcat cggccagggc gtcgcggtag gcgcggaagt tggggcgttc ggggtggcgg
      541 gccacctgga cccgctggta aggggtgagg ttggagtaaa tacgccttct cgcggcgtcg
      601 agggcctcct cgaggcgtga gatctccccc cgcaactcct cgctcgggcc ggcgagccgc
      661 ctgagctcgg tgatgcggtc ctcgaagtcc ttgatgggcc tctcgaagtc caggatcacg
      721 gcaagaaccc cagaaggcgt tcgagatcgg ccttcaggct gaggcggtgc acgatgcggt
      781 cgaccatccc atgctctttc tggaactccg ccgtctggaa gccttcggga agcctctcct
      841 ttgtggtctg ctcgatgacg cgcgcccccg tgaagcctat cctggccccc ggctcggcaa
      901 gcaccacgtc ggccgccgtg gcgaacgagg cggtaacccc gccgaaggtg gggtcggtca
      961 acaccgagat gtatggccac gacccctcca cgtagcgcga gagggccacg ctggtcttgg
     1021 ccatctgcat gagcaagtag acgccctcga acatcctggc gccgcccgaa gccgatacga
     1081 taacgagcgg caactcctcg ctaccggcaa gctcgacggt cctcgcgacc ctctcgccga
     1141 ctacgctccc catcgaacca ccgatgaagc tgaaatccag cacggcaaga gccacccgct
     1201 tgccacccac ggcaccggtc ccgctcagga tggcgtcatc gaggcccgtt cgtccacgcg
     1261 ccttctggag cctctggcgg tagttctcga aaccaagaag gtcatcagcg aggatgcctg
     1321 cgtccctctc ctcgaagctg cccgagtcgg taaggagacg gattctctcg gaggcggaga
     1381 gcgggtagtg gaactcgcag tgggggcaga cgttgaagcg ggccctgagg tctttctcgt
     1441 agatgggctc cccacacttc tcgcacttgg tgaagacgga gtccatcggc ccgacgccgc
     1501 tctcggtctc gggaaccgtc ctggagtact cccggcgttt gctgagccag ttcctcaagc
     1561 ccggcgcccc gtcttggtga ccccggagag cgcctcgcgc accccgcgga gccacgtgcc
     1621 cgcgtgcccg gctccaccct cctcgacgag ccgcatgagc ttgctcccga tgatgacccc
     1681 atccgcttcg ccggcagcct cgacggcggc ctccgccgac ccgatcccga agccgagggc
     1741 cacgggagcc tcggttctcc cccggacccg tctcaatagc tcgacggcgc cgggcggaag
     1801 ggtatcgcgt acccccgtca ccccggccac cgagacgcag tagacgaagc ctgtggccat
     1861 ctcgccaacg cgcgagatgc gctcatctgt cgaggtcggg gcggccagcg ggcagaaggc
     1921 gataccggcc tcgtctgcga gcgcggcgaa gtgaccagcc tcatccacgg gaagatctgg
     1981 aatgacgagg ccggagacgc ccgcgtcggc cgcctcgcgc aggaaacgct ccgggccgcg
     2041 ggcgaagatc acgttgtagt aaagcaggaa gaccacgggc acacgccccg agaatcctga
     2101 ggcgaggtcg agacagtagt cggggtcggc cccgttctgc agggcgagcg tggtcgtgtt
     2161 ctggacggtc ggcccgtccg cgagcgggtc tgagaaggga acacctatct ccagtacgtc
     2221 gccgccggcc tccagaaagg cctcccccac ctcccgtgct ccctccagcg aggggtagcc
     2281 cccggtcagg taaggcacga gcgccgtacc cccctcccga aaagccgccc gcacgtgctc
     2341 gccgctcaaa aggccgcttc gtccgtctcc gaggtggtcg acggcgcctc cgagtcggag
     2401 acggcctccg cctcctccgc gtcgattccg agcgcgttgg ccgcgacctc aacgtccttg
     2461 tcaccgcgtc cggagatgtt gacgaccagg ttcttgccag gcccgagctc cctggccacc
     2521 gtctctgcgt agtagagcgc gtgggacgtc tccagcgccg gaattatgcc ttcagccctt
     2581 gagcactcct ggagcgcctc gagcgcctcc ttgtcggtca cgctcgcgta ctcggcaagg
     2641 ccctcgtctt tcaggaaggc gtgctccggg ccggttgcgg ggtagtccag gccagccgag
     2701 atggaatggg cttcgcggat ctggccagca tccgtctgca gcacgtagct catcgcgccg
     2761 tgcaggatgc cgaggttccc ctcggcaagc gacgcgccgt gctcaccgct ggagagcccc
     2821 cgtccggcgg cctcgacgcc aacgagccgc acgcccttac ggccgacgaa ggggtggaac
     2881 gcgccgatgg cgttcgagcc ggcgcccacg caggcgacga tggcgtcggg gtccgaacca
     2941 aggcgctcgc ggcactggtt ctctatctcc gaacctataa cggcctggaa gtcgcgcacg
     3001 atgcgggggt agggcgcggg gcccacgacg ctcccgataa tgtagtgggt gtcctcgacg
     3061 ttggtgaccc agtcccttat ggcctcgttc acggcatcct tcagcgtccg cgaaccggag
     3121 agcactggca cgacctcggc cccgagaagc ttcatcctca ccacgttcag cctctgacga
     3181 cgggtgtctt cctcgcccat atagaccacg cagtccttgc cgtagagggc ggcgaccgtc
     3241 gcggtggcga cgccgtgctg gccggcgcca gtctcggcga tgatacgctc cttgcccata
     3301 cggtcggcaa gcaggatctg acccagggtg ttgttgatct tgtgcgcccc ggtgtgcgcc
     3361 aggtcttcgc gcttgaacca cacgttcgcc ccgccccact tgcgggtcag acgttcggcg
     3421 aacataaggg gggtcggacg gccgacgaag tccctcgcca gacggtccct atcctcctcg
     3481 aactcgggat cgttcctgta ccgctcgtaa gcctcttcga gctcttcgag ggcgtggatc
     3541 agggtctcgg gaacgtagcg gcccccgaag ggaccgaagt aacccttatc agtttcttgc
     3601 cgcaaggacg aacctccgca ccaactcgcc gttttttctg cccggcccgt cctccaggga
     3661 actcgatgcg tcaaccccga agggcttgaa gaactcgacc gcctcgcgca cgttctccgg
     3721 gccaagcccc ccggaaacga cgatgttatc acgtcctctc agagacttcg ccacccccca
     3781 gtcgaaccgc tctcccgtgc ccccccgagc ccgtgcgctg tacgcgtcga gcaggaacag
     3841 atctgcctcg tactcgtcga tcgcctcgag ggtggccgca ctccggaccc gcagcgcctt
     3901 catcactttg acgcctgcct cccgcaggtc ggttacggtc tcgggtgtct cgtcaccgtg
     3961 caactgaacg aggtccaacc cgacctcctg cgagatcctc aatatcttcg ccggttcttc
     4021 gtcgacgaag acgccgaccc tgatcgtgtc ctcgggcaga gcaacggaga tgctccgcgc
     4081 ctcttcgatg ccaacgtgcc gtgggctccc ggcgaagatg aacccgacgg cgtcggcgcc
     4141 tgcctcggcc gccaggcggg cgtcaccggc gttggttatg ccgcaaatct ttacgaacgt
     4201 cataggccag agtgtactct ctaaagcgcc aggcgatcgg cgatcggttc ccggtttgtt
     4261 gccgaaagag cgtagccgtg aggacgcagg ttgcgaagcg gaccgcccca tagattacta
     4321 tgctctcatg gagacaacgg agacacttgc cgggggctcg gacggacctc ggccggaagg
     4381 gagcatcttt gcgaccggag gatatcgttg cgcgggcggc gacggtgttg cgagagaacg
     4441 atatgggcgg atggaccagg gcctctccaa cgctctaccc ccaccagtgg agttgggact
     4501 cggcgttcat cgccatcggt ctggctcacc tcgacactgg tagggccgcg caggagatag
     4561 gctccctgct ggaccaccag tgggacaacg gcaagatccc gcacatagtc ttcaaccccc
     4621 acgcgccccc gcagagttac tttccgggcc ccgagcactg ggcgagcgcc gctgatgccc
     4681 ctgacgcccc cacgggcccg gccacgacga gcgggctctg ccagccgccg gtgcatgcca
     4741 tagcggccct gcgtatcctc cgcgtcgccg aggcgcgggg cgagggcgtc gaggaggcca
     4801 gggcgtttct cggcgaggtc tacacgaagc ttctggcctg gcaccggtac cttgccacca
     4861 gccgcgatcc cgagggttcc ggcctcgtaa ccatctttca tccctgggaa agtggcacgg
     4921 acaactcccc gcgctggacc cccgccctgg agcgggtcga gatcgggaag ataccctact
     4981 acgagcgcct ggaccttggg catgtggacc cctccgagcg acccacgggc tacgagtacg
     5041 accgctttat ctggctcgtc gacatcatca agggagcaga ctgcgacgac gaggtgctgt
     5101 tcaccaaatg tcctttcatg gccaaagatg ttctggccag cgccatcctg gtaagggcca
     5161 acgaggcgct cgtggagata tccgattgtg taggagcccc ggaggaggac cgggccaccc
     5221 tccgcgcctg gatcgaacgc ggtcgcaagg gcctggacgc gaggtggaac cccgatctcg
     5281 gcctgtgctc ggactatgac ctccgcgccg gcgaaacgct cccggcccgc actatcgccg
     5341 gcttcgcgcc gctgatcgcc ggcagcgagc gggaggacca actcgaggcc ctgatgctga
     5401 cgctcgactc gccgctgttc gcgggagacg aggacctccg ctggcccctc ccacccagta
     5461 ctagccccca ggagaaggga tttcttccta gaagctactg gcgcggtccc gtatggcccg
     5521 tgatcaactg gctgctctgg tggtctctgg acggcgcggg tgagaggaag agggcggacc
     5581 ggctcaaggc ctactctctg gcccaattgt ccgacgggtg cttcggcgaa tactacgagc
     5641 cgttcaccgg cgaggcgctg ggctcagacg accagtcgtg gaccgcggcg gtggccctcg
     5701 actggctgtc ggatgaggaa ctaaacggca gcctggcccg gtgacggggt tgcgagtagg
     5761 ggacgcgttc cccgacttcc gcctgcccga ccaggacgga cgccgtcgcc ggctctcgga
     5821 ctacacgagg ccaggccccc tcgacgagag gttgggcttc gacgacgggt atcccctggt
     5881 cctggtcttc ggacgcggct tcttctgccc cagggaccag gagcagatgc gcggcctcgt
     5941 ccggttccag tcggagctcg cggtcaacta ctgcaagctc atcagcgtga gcgccgactc
     6001 ccagagggtg tcggccgcct tcagggccgg cctcggggcg cggtggccct tcctctcgga
     6061 cgaggggcgc gaagttatca ggagcataga tatactagac gagaccgagg gcgagtatgc
     6121 cggggtctct cgccccttca ccttcgtgct ggcacccggc ctcgagatcc acaggatcta
     6181 cgacggctgg ttcttcgtcg ggaggcccac gttggaagag ctgcggcagg acctgcggga
     6241 gatcatgcgc acacgcaaag attaccgtta cgaggcatac gacaaccccg gggtaaagag
     6301 gatccgtatt ccccaacagg aatgggccga tgggccacca cccct
//
LOCUS       DBNGVD010000203         2998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1499893, whole genome shotgun sequence.
ACCESSION   DBNGVD010000203 DBNGVD010000000
VERSION     DBNGVD010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1499893"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16CM_05675"
     CDS             <1..513
                     /locus_tag="AC16CM_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR3139353.1"
                     /translation="IERYARDGYLGESGYYANNWPAWRAENDEEIRRMLSGEEKLPLE
                     RSTEYAADVIEAITLGGEKVVYGNVENTGLIENLPDGCVEVACLVNRNGVQPTHFGAL
                     PEQLAALNRAHMAVHELVVQALLERDRQAALYALMLDPLTAAVCSLEEIRNMFDEMWE
                     AQRPFLTSFQ"
     gene            complement(615..1082)
                     /locus_tag="AC16CM_05680"
     CDS             complement(615..1082)
                     /locus_tag="AC16CM_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3139354.1"
                     /translation="MSGDVSPVPRGFHTVTPYMTIKGAYEAIEFYKEAFGAEEIFRWA
                     DPDGRVRHAEVMIGDSPIMITDEAPDFGMYGPRSSGGSPVHIFLYVDDVDATFEKALA
                     AGATELMPVENSEDGDRRGGVTDPFGHVWYIATHVEGISRDDLQRRYDTAANQ"
     gene            complement(1193..2371)
                     /locus_tag="AC16CM_05685"
     CDS             complement(1193..2371)
                     /locus_tag="AC16CM_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3139355.1"
                     /translation="MLLAAQGLMSPPGHSAQKADVLEAVRRMGVLQIDSISVVARSPY
                     LVLWSRIGAYDPLWLDELLAEGKIFEYWSHAACFIPIEDYGLYRRLMLDETDKTRVWM
                     EAHPDALKHVMAHIRKEGPVRSAEFARTDGKAGGWWEWKPEKRALEHLFAAGELMISH
                     RENFQRVYDLRERVLADALPTWEDAFAPTEHEVRRALALKAVRALGVAVARWVPDYFR
                     TPKRGAASLLEGLADDGSLLRAEIEDEPAYVHPENARVAESILSGRTKSSATTLLSPF
                     DPVVWDRDRASELFGFDYKIEVYTPAARRRYGYYSLPILHDGALVGRLDAKAHRKEGL
                     FEVKAAHLEPDVVASEHLVSSVAGALRDCAAWHGAPEVLVREARPSEVGGALRVALEV
                     "
     gene            complement(2565..>2998)
                     /locus_tag="AC16CM_05690"
     CDS             complement(2565..>2998)
                     /locus_tag="AC16CM_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR3139356.1"
                     /translation="SRLREVFERLRPRLRTFSDQGGTELFDLPDAPRPDPEVPAPVRF
                     VAAFDNLILSHADRSRVIAAEHRKALASRNGMVPATVLVDGFVCGTWKVVRERGETTL
                     WITPFEPLPKQDREALMEEGERLVSFVTDGAGDFAVRFFEP"
BASE COUNT          541 a          986 c          952 g          519 t
ORIGIN      
        1 atcgagcgtt atgcccggga tggatacctg ggcgagagcg gctactatgc caacaactgg
       61 cccgcctggc gggccgagaa cgacgaggag atccggagga tgctctccgg cgaggagaag
      121 ctacccctcg aacggagcac cgagtacgcc gccgacgtga tcgaagccat cacgctgggc
      181 ggggagaagg tggtctacgg caacgtagag aacacgggcc tgatcgagaa cctgcccgac
      241 ggctgcgtgg aggtcgcctg cctggtgaat cggaacggcg tccagccgac ccacttcggc
      301 gccctgccgg aacaactggc cgccctcaac cgcgcgcaca tggccgtcca cgaactggtc
      361 gtccaggctc tgctcgagcg cgaccgtcag gccgccctgt acgccctgat gctcgatccg
      421 ctgacggcgg ccgtgtgctc gctggaagag ataaggaaca tgttcgacga gatgtgggaa
      481 gcccaacgcc cgtttctaac ctcgttccag tagaaccatc gtagagcgag attcaggcca
      541 tcagctcctc gttcttgctg acagcggtcg ttcacgaccc tgacgccgaa cgcctcgcct
      601 accggccttt gcggctactg gttggctgcg gtgtcgtagc gccgctggag atcatcgcga
      661 gagatgccct cgacgtgcgt ggcgatgtac cagacgtgtc cgaaggggtc ggtaacgccg
      721 ccccggcggt cgccgtcctc actgttttct acgggcatta gctctgtggc accggccgcg
      781 agcgcctttt cgaaagtcgc gtccacgtca tcaacgtaga ggaaaatgtg taccggagag
      841 ccgccggacg accgcgggcc gtacatcccg aagtccgggg cctcgtcggt gatcatgatg
      901 ggagaatcac cgatcatgac ctccgcgtgg cggacgcggc cgtcagggtc agcccagcgg
      961 aagatctcct ccgccccgaa cgcctctttg tagaactcga tggcctcgta cgcgcctttg
     1021 atggtcatgt acggcgtaac ggtgtgaaaa ccccggggta cggggctgac gtcgccactc
     1081 atgcgtcccc tttcctgcac tacgacacag accgagtgta cgtccggacg gcctacttcc
     1141 catcctccga atccgccgca ctagaagcct ccgcgagccc atcggggccg ggtcaaacct
     1201 cgagcgccac ccggagcgcc ccgccgacct cagaagggcg agcttcacgg accagcacct
     1261 ccggcgcccc gtgccatgcc gcgcaatcgc ggagcgcacc ggcaacgctg gatacgaggt
     1321 gctcggaggc cacgacgtct ggttccaggt gcgcggcctt tacttcgaag aggccttctt
     1381 tgcggtgggc tttggcgtca agccgaccca cgagggcgcc gtcgtgcagt atggggaggg
     1441 agtagtatcc gtagcggcgc ctggcggccg gggtgtagac ctcgatcttg tagtcgaagc
     1501 cgaagagctc ggaggccctg tccctgtccc agacaaccgg gtcgaagggc gacaacaggg
     1561 tcgtggcgga agatttcgtc cggccagaga ggatactctc ggccaccctc gcgttctcgg
     1621 ggtgcacgta ggccggctcg tcctctatct cggcccgcag cagggaacca tcgtctgcca
     1681 gcccttcgag tagcgacgcg gcccccctct ttggcgtgcg gaagtagtcg gggacccagc
     1741 gggcgactgc cacacccagg gcccgcacgg ccttcagtgc cagcgcccgc cgtacctcgt
     1801 gttcggtcgg ggcgaaggcg tcttcccagg taggcagggc gtcggccagc acgcgctcgc
     1861 gcaggtcgta gacgcgttgg aagttttcgc ggtgcgagat catcaactcc ccggcggcga
     1921 agaggtgctc gagcgcgcgt ttctcgggct tccactccca ccagccgccg gccttgccgt
     1981 cggtacgggc gaactcggcg gaacggacgg gtccttcctt tcggatgtgt gccatgacgt
     2041 gcttcagcgc gtcagggtgg gcctccatcc acacccgcgt cttgtccgtc tcgtcgagca
     2101 tcagacgccg gtagaggccg tagtcctcta taggaataaa gcacgccgcg tgcgaccagt
     2161 actcgaagat ctttccctcg gccagaagct cgtccaacca gaggggatcg taagccccta
     2221 tcctgctcca cagcaccagg taggggctgc gtgccaccac agagatggag tcgatctgca
     2281 acacccccat cctgcggacg gcctcgagta cgtctgcctt ctgcgcggag tgcccgggcg
     2341 gtgacatcaa cccctgtgcg gcgagcaaca tcgtgcgcgc cgcgtccctc gaaaactcga
     2401 tccttcacac ctcctgaccg gctacggcga actgtggagc tactagcctt tgctgaaagc
     2461 cgaccgcagg tcgcttgcaa agcaggttcg gcatacatgc caaccgtcgt agttatacgg
     2521 caaccggcgc ggtcctacga gtatcaagac ctcataaggg ttctctaggg ctcgaagaac
     2581 cgaaccgcga agtctcccgc gccgtccgtc acgaagctca ccagtcgctc tccctcctcc
     2641 atcagggctt cgcggtcctg cttcggcagg ggctcgaaag gtgtgatcca gagcgtcgtt
     2701 tccccgcgtt ctcgcactac tttccacgta ccgcagacga agccgtcgac gagaacagtg
     2761 gcaggaacca taccgttcct ggaggcgaga gctttgcgat gttcggcggc gatgacgcgg
     2821 gagcggtcgg cgtgagagag gatcaggttg tcgaaggcgg ccacgaagcg caccggagcc
     2881 ggcacctcgg gatcgggcct cggggcgtcg ggcagatcga agagctcggt acccccctga
     2941 tcgctgaagg tacgcagccg cggacgtagt cgctcgaaga cctctcggag cctgctca
//
LOCUS       DBNGVD010000204         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2774786, whole genome shotgun sequence.
ACCESSION   DBNGVD010000204 DBNGVD010000000
VERSION     DBNGVD010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2774786"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="AC16CM_05695"
     CDS             <1..957
                     /locus_tag="AC16CM_05695"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3139357.1"
                     /translation="EQRGVLRVLKADGTLTTFLDISGKVDAAGERGLLGVAFDPAFPE
                     NHYVYLYYTRKSTSTNPAHNRVVRVTADGNRAVSGSERLILRLNNLSSATNHNGGAIH
                     FGEGGKLFVAVGDNADGTNAQSLRTLKGKMLRINKDGTIPRGNPFYERAAGNNRAIWA
                     LGLRNPFSFAVQPRTGEMFINDVGQSTWEEINRGLAGENYGWPRYEGPESDPAYRGPV
                     FAYAHGDFGTTGCAAITGGTFYDPPTAQFPSRFAGDYFFADLCGGWIRRLDGATGDVR
                     SRGFATGISRPVDLKVSKNGVLYYLSRGEGSGSVGKIRYTGT"
     gene            complement(1013..1354)
                     /locus_tag="AC16CM_05700"
     CDS             complement(1013..1354)
                     /locus_tag="AC16CM_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139358.1"
                     /translation="MTEAASDRQEIERRIVRRSLEDEAFRQQLLDDPRAAIGEEIGAP
                     LPDEVQVVAVEETADTIYLVLPHSGSSAGQDPEEVSDQDLEAVAGGGVATQNTCVSGE
                     NTCSTCGGWSC"
     gene            complement(1415..1744)
                     /locus_tag="AC16CM_05705"
     CDS             complement(1415..1744)
                     /locus_tag="AC16CM_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139359.1"
                     /translation="MSDTTGRQEMERRIVERSLQDEDFRRRLLEDPKATVEEELGVRL
                     PDEVGIVTLEETADTIYLALPSRSPAGQEDLSDQELEAVAGGGVPHSAGTCAGQSTCD
                     TCGQWGC"
     gene            complement(1835..2188)
                     /locus_tag="AC16CM_05710"
     CDS             complement(1835..2188)
                     /locus_tag="AC16CM_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139360.1"
                     /translation="MSEASGGGGRAEIERKIVERSLQDEVFRRRLLADPRATLEQEYG
                     ERIPEGVQVRAVEETADTIYLVLPSSSAGAGAGGLSDQDLEAVAGGDTSWTCSGWSCG
                     PPDQCQTAVGPGCTS"
BASE COUNT          413 a          887 c          745 g          438 t
ORIGIN      
        1 gaacagcgcg gcgtgcttcg ggtgctgaag gcggacggga ccctgaccac gttcctggac
       61 atctcgggca aggtggacgc ggcgggcgag cgcggcctgc tgggcgtcgc cttcgacccc
      121 gccttccccg aaaaccacta cgtctacctc tactacacaa ggaaatcgac gtccaccaac
      181 ccggcacaca atcgggtggt tcgcgttacg gcggacggca acagggccgt ctcgggcagc
      241 gagcggctca tcctcaggct caacaacctg agcagcgcca ccaaccacaa cggcggcgcc
      301 atacacttcg gcgaaggagg caagctcttc gtggccgtcg gtgacaacgc agatggcacc
      361 aacgcgcaat cactgcgcac cctgaaaggg aagatgctcc gcatcaacaa ggacggcacg
      421 atcccccggg gaaacccctt ctacgagcgc gccgcgggca acaaccgcgc catctgggcg
      481 ctcgggcttc gcaacccgtt cagcttcgcg gtccagcccc ggacgggaga gatgttcata
      541 aacgacgtgg ggcagagcac ctgggaggag atcaaccgcg gcctggccgg ggagaactac
      601 gggtggccca gatacgaggg acccgagtcc gaccccgcct accgtggccc cgtcttcgcg
      661 tacgcgcacg gcgacttcgg caccacggga tgcgccgcca taaccggcgg gaccttctac
      721 gatcccccca cggcacagtt cccgagccgc tttgctggcg actacttctt cgcggatctc
      781 tgcggcgggt ggataaggag gctcgacggc gccacgggag acgtccggag ccggggcttt
      841 gccaccggca taagccggcc cgtagacctc aaggtgagca agaacggtgt cctctactac
      901 ctcagcaggg gagaaggatc gggctcggtc ggaaagattc gctacacggg gacctgagtc
      961 ggcccgtgaa aagagtcaac ccgagaaggc tacaggtgat gtacgtggcc cgttagcagc
     1021 tccacccacc gcaggtgctg caggtgttct cacccgacac gcaggtattc tgggtggcga
     1081 cccctccacc ggccaccgct tcgaggtctt gatccgagac ctcctcgggg tcctggccgg
     1141 ccgatgatcc gctgtggggg agcaccaggt agatggtgtc cgccgtctcc tccacggcaa
     1201 ccacctgtac ctcatcgggc aacggcgcgc cgatctcctc cccgatcgcc gccctgggat
     1261 catctagaag ctgctggcgg aacgcttcgt cctccaggct ccgccggact atccggcgct
     1321 cgatctcctg gcggtcgctg gccgcttcgg tcatgatctt ccccctttgc tctcgctcgt
     1381 cctgcttgcc gcaagtgcct ctgaaggctg cccgtcagca accccactgc ccgcaggtgt
     1441 cgcaggtaga ctgccccgcg caggtgccgg cgctgtgcgg tacgccgccg ccggctaccg
     1501 cctcgagttc ctggtcggag aggtcttcct gcccggctgg cgacctgctg gggagcgcca
     1561 ggtagatcgt gtcagcggtc tcctctagcg tcacgatgcc cacctcatcg ggcaaccgaa
     1621 cgccaagctc ctcctctacc gtagccttcg ggtcctcgag tagccttctc ctgaagtcct
     1681 cgtcctggag gctacgctcg acgattcttc gctccatctc ctggcgacca gtcgtgtcgc
     1741 tcattcagct ctcccctttc tcgtctgccc tggtcttcgt cggatgccca caccacgtcc
     1801 tggcacggtg agagcgctac atgagactag ctgctcaact cgtgcagccg ggcccgactg
     1861 cggtctggca ttgatccggc gggccgcagg accagcccga gcaggtccag gaggtatcgc
     1921 cgccggccac cgcttccaga tcctggtcgg agagaccgcc ggccccggct cccgccgagc
     1981 tgcttggaag caccaggtag atggtgtctg cggtctcctc cacggccctt acctgcaccc
     2041 cctcgggtat acgctccccg tactcctgct ccagggtggc ccttggatcg gcgagaagcc
     2101 ttctcctgaa tacctcgtcc tgaaggctac gctcgacgat cttgcgctcg atctccgcgc
     2161 ggccgccgcc cccacttgct tcgctcaaga ttcctactcc tttcgcatcg accacggatg
     2221 agcctgaccg tggccttcgc gcattgctaa tccgaccacg gcctcgtcga aagtacatcc
     2281 tgacggccac ttgcggcacc tcgtggtgcc tctggtgtcc cctcaggagt tcggcttcag
     2341 gcgcccagct cgccaccgac gggcgagaat ccggcatcac cctctgggtc ggcggggtac
     2401 accgctccac ggtagccctg ggaccggcaa gcggccttcg ctgccgctac gccttcaggg
     2461 ctaccgtggg atgaacctcc ggt
//
LOCUS       DBNGVD010000205         5193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1136745, whole genome shotgun sequence.
ACCESSION   DBNGVD010000205 DBNGVD010000000
VERSION     DBNGVD010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1136745"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16CM_05715"
     CDS             <1..506
                     /locus_tag="AC16CM_05715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139361.1"
                     /translation="TLEDTSLEMWRDVFELNLFAAVDLVKRVAPGMRERGWGRIINIS
                     SENGTQPYPDMISYSASKGALDNFSKALSKQYAADGILVNTVSPAFIETPLVNEMMEQ
                     AAEEQGISTDSAVRQFLENNRPHIELGRPGRIDEVGPIVAFLASEKASFINGSNYRVD
                     GGSVASI"
     gene            754..1500
                     /locus_tag="AC16CM_05720"
     CDS             754..1500
                     /locus_tag="AC16CM_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3139362.1"
                     /translation="MSLALLTILGLSILPNACKAGSAPAIVGGTGDNELRGTPQADSI
                     RGLAGEDRIEGLEGDDVLHGMPGRDTLYGGEGRDAVDGGSYADRASGGPGIDLLVGYD
                     GQDWLYGGGKPDALWGGKDDDHLYAGGGGGFASGGPGEDEVVGGDGRDRLDGEADDDE
                     VSGGPGEDRLEGGMGEDLLDGQDGDDSLFGEIDRDTIRGGNGNDFIDGIDGISGDRID
                     CGPGPADIAQIDAGDEATSCETLRTVGGRR"
     gene            1663..4308
                     /locus_tag="AC16CM_05725"
     CDS             1663..4308
                     /locus_tag="AC16CM_05725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3139363.1"
                     /translation="MEETSYVQRSRVGRAPWSRVFEGTRLTRALWVLNLVIVALTLLL
                     VFLTPAFLLPAERPAAVLVVVSLFLSLACSSVGVLVASHSPRNPVGLVFCVMGSSYGA
                     WRLSVACADYALLVRSGGPMGEVAAWTSAWLWFPVVASGALLVLLYPDGGLPSPRWRP
                     AVWAVGGGAVLATFGTALRPGPLLAYYYVSNPYGVIGGTSPAGFLVEASTILGGVLLC
                     AGCLAAIAALALRLTDGQGRRHLGWFACATFPALLIPAAVLATWSVERFGLLVLGRTF
                     SPLLRAVEASVLSVEPDDMAGAVIALSVAASLEFLGACAILLVPIFAFVGMRRHGLYG
                     LDSAAPLAASRWFRVFAGGIVAGALPFAFVYLAVFLYVILYLVVRQHQPEQEHLGWIV
                     TFVGGWGALAFFVAVTFLVAFLVSRRAESRSVLLGTLVGLVATAVQVVLFLVDPPIVP
                     GGFLVYLCLGLAGGYFGGLAGRSTLSGGLYRVSRRIGAARDAAAVAAVIGENLGGPGV
                     DGVAVWRRDGPESRPTEAYGVGPRGDDGERAELWGSWRAEGGEGWPPGLGPAEVEAAV
                     LPTPGERSWATVQRSQLSPEEKRAWEHSGVRSALLVPLIVPGKAWSGLLMVTFRKRLR
                     FSGRAASAYLAVASQAALVLENLRLIEEARQTGRRGGILLERQRLAREIHDTLAQGFT
                     GVITSLTAAELSSDASEMGADSARYLRDAKRIAGDSLAEARRLVWALRPEALDRYSLS
                     EALSNLVRQWSEQSGIEARGTTNGTPRELLPEAEVALLRAAQESLTNVQKHAHASRVD
                     VTLTYMDDRVVIDVVDDGVGFDPAETRPVIGPQDERGFGLTAMRERVEQIGGKLAVET
                     APGEGTAIAVELRDGEVPRTIEEVH"
     gene            4305..4946
                     /locus_tag="AC16CM_05730"
     CDS             4305..4946
                     /locus_tag="AC16CM_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139364.1"
                     /translation="MTEQRTRILVCDDHPVVRSGLRGMIQSQPDLEVIAEAGDGAQAV
                     ALAGRLRPDVVLMDLRMPGMDGVAATASIKERHPAIHILILTTYDTDADILRALDKGA
                     TGFLLKDEREDRLFDAIREAADGRSTLAPGVASRLVERFRGNADSHVSEREIEILMLV
                     AQGWSNREIARELHISDSTVKAHMLHIFDKLGVTDRTAAVTTALRQGIIRLEP"
BASE COUNT          836 a         1567 c         1855 g          935 t
ORIGIN      
        1 gcaccctgga agacacctcc ctggagatgt ggagggatgt cttcgagttg aacctcttcg
       61 cagcggtaga tctggtgaag cgcgtcgctc ccggcatgcg ggagaggggt tggggaagga
      121 tcatcaacat ctcttccgag aacgggacgc aaccctaccc ggacatgatc tcctacagcg
      181 cctccaaggg cgccctggac aacttctcaa aggccctctc caagcagtac gccgccgacg
      241 gcatcctcgt caacacggta agccccgcct ttatcgagac ccccctggtg aacgagatga
      301 tggagcaggc cgcggaggag caggggatct cgaccgatag cgccgtcaga caatttctgg
      361 agaacaaccg tccgcacatc gagctaggtc ggcccggccg catcgacgag gtcggcccga
      421 tcgtggcgtt cctggcatcc gagaaggcgt ccttcatcaa cgggtccaac taccgcgtgg
      481 acggcgggtc ggtcgcgtcg atctagaaga aaggcgttcg accgggagga gatcaagagg
      541 ctctcatccg ttttgaggtt gtgtgttcct gtcatgctcg tcgatctctt tcagctcgtg
      601 gcactcggac cgcggcaggt ctggacagga cggccgatcc cggctagggt gatatttgtt
      661 ctccagccct ggcgcgatgg acctggttga aggatgtatg catctcgaac acgatgtcga
      721 atcggtggcg gatggccccg gagacggggc gcattgtcgc tggcgctcct gacgatactc
      781 gggctatcta tcctgccgaa cgcctgcaaa gcggggtccg cgcccgccat agtaggggga
      841 accggagaca acgagttgag aggcacaccg caggccgact caatacgcgg ccttgccgga
      901 gaggaccgca tcgaggggct ggagggcgac gatgtcctgc atggcatgcc cggccgggat
      961 acgctgtacg gcggcgaagg ccgcgacgcc gtcgacggag ggtcatatgc ggatcgtgcg
     1021 tctggtggcc ccggaataga cctgttggtc gggtacgacg ggcaggactg gctgtacggc
     1081 ggcgggaaac cggatgccct gtggggtggc aaggacgacg accacctgta cgccggtggc
     1141 gggggcggtt tcgcaagcgg cggtcccggc gaggatgagg tggtgggcgg cgacgggcgg
     1201 gaccgcctgg acggcgaggc tgacgacgac gaggtctccg gcggcccggg cgaggatcgg
     1261 ctggagggtg gaatgggcga ggacttgctc gatggccagg acggcgacga ctccctgttc
     1321 ggagagatag accgcgacac catacggggc gggaacggca acgacttcat cgatgggatc
     1381 gatggaatct ccggggacag gatcgattgc ggtcccggtc ctgcggacat cgcccagatc
     1441 gacgcgggag acgaggcgac cagctgtgag accctccgga ccgttggggg ccggagatga
     1501 aggtcggagg ggaaggagtt ctggctacct tccggtcgtt cgccgcactg gcagcgggcg
     1561 ggatggatcg tgtaccctga tgcaagcggt ggcgcggggg agattggtga tgcgggtgcc
     1621 tgctcacgct ccttccggaa gggaaacaac ataggtttgg gtatggagga gacatcctac
     1681 gtgcagagat cgcgcgtggg ccgcgccccg tggtccaggg ttttcgaggg cacgcggctc
     1741 acccgggctc tatgggtact gaacctggtg atcgttgctc tgacgctact gctcgtcttc
     1801 cttacgcctg ctttcttgtt gccggcagag agaccggcgg cggttctggt ggtggtctct
     1861 ctcttcctgt cgcttgcctg ctcgtcggtg ggcgtcctgg tcgcctccca ctcgccgcgc
     1921 aacccggttg gactggtctt ctgcgttatg gggtcatcct acggtgcctg gcgtctctcg
     1981 gtagcttgcg cggactacgc tctactcgta cgttccgggg ggccgatggg agaggtggca
     2041 gcgtggacct cggcgtggct ctggttcccg gtggtagcct ccggggcgct gcttgtgctc
     2101 ctctatcctg acggcgggtt gccctccccc cgctggcgtc cagcggtgtg ggccgtgggc
     2161 ggcggtgccg tcctggccac cttcgggacc gcgcttcggc ccggtcccct gcttgcgtac
     2221 tattacgtaa gcaatccgta cggggtcatt ggggggacgt ctccagcggg cttcctcgta
     2281 gaggcgtcga cgatccttgg cggggtcttg ctctgtgcgg gttgcctggc ggcgatcgcc
     2341 gcgctggcgc tgcgcctcac cgacggtcag ggccgtcgac acctcgggtg gttcgcgtgc
     2401 gccacctttc cggcgctact cattccggcc gcggtcctgg cgacctggag cgtcgagagg
     2461 tttgggctgc tcgtgctcgg caggacgttt tctccactgc tgcgtgccgt cgaagcctcc
     2521 gtgctttccg tcgaaccgga cgatatggcg ggggcggtga tcgcgctaag cgtggctgca
     2581 agcctcgagt tccttggcgc ctgtgccatc ctgctggtac cgatcttcgc cttcgtgggc
     2641 atgcgcaggc atggcctgta cggcctggat agcgcggcac cactcgccgc gtcgcgctgg
     2701 ttccgggtgt tcgccggggg catcgttgcg ggtgccctgc cattcgcgtt cgtctacctg
     2761 gcggtcttcc tctacgtcat cctctatctc gtcgtgcggc aacaccagcc ggagcaggag
     2821 catctcgggt ggatcgtaac cttcgtgggc ggttggggtg cgttggcgtt cttcgtagcg
     2881 gtcacgttcc tcgtggcctt cctcgtatcc cggagggccg agtcgcggtc cgtgctcctc
     2941 gggacgctcg ttggtctggt tgcgacggcc gtacaggtgg ttctgttcct cgtcgatccg
     3001 cctatcgttc ccgggggctt tttggtttac ctgtgcctcg ggctggccgg cggctacttc
     3061 ggtggactcg ccggccgctc caccctctcc ggcggtctgt acagggtcag ccgccggatc
     3121 ggcgcggcaa gggatgccgc ggcggtcgcc gccgttatcg gcgagaacct cggcggtccc
     3181 ggggtcgatg gcgtcgccgt gtggaggagg gatggtcccg agagccgtcc cacggaggct
     3241 tacggcgtgg ggccgcgagg ggacgacggg gagcgggccg agttgtgggg ctcatggagg
     3301 gccgagggag gcgaaggctg gcccccaggg ctcggtccgg cggaggtgga agcggccgtg
     3361 ctcccgacgc ccggggagcg gtcctgggcc acggtgcagc gatctcagct gtccccggaa
     3421 gagaagcggg cgtgggagca ctcgggggtt cgttcggcgc ttctggtccc cctcattgtt
     3481 cccggcaagg cctggagcgg gctgttgatg gtgacttttc ggaagcggtt gcgcttctcc
     3541 ggccgcgcgg caagtgcgta cctcgcggtg gcctcgcagg ccgccctggt gctggagaac
     3601 ctgcgcctga tcgaagaggc caggcagacg ggacgccggg ggggaatcct cctcgaacgc
     3661 cagaggctgg cgcgggagat acacgacacc ctggcccagg ggttcaccgg cgtcattacg
     3721 agcctcaccg ccgcagagct aagcagcgat gcttcagaga tgggcgcgga ctcagcacgt
     3781 tacctgcggg acgccaagcg catcgccggg gacagcctgg ccgaggctcg caggctggtg
     3841 tgggccctgc gccccgaggc gttggatcgc tactcgctct ccgaagcgtt gagcaacctg
     3901 gtccgccagt ggtcggagca atccggaatc gaggcacgcg ggaccacaaa cggcacgccg
     3961 cgcgagctcc tcccagaggc ggaggtggcg ctcctgcgcg cggcgcagga gtcgctgacc
     4021 aacgtccaaa agcacgccca cgccagcagg gtcgacgtaa cactcacgta catggacgac
     4081 cgggtcgtaa tcgacgtggt cgacgacggc gtcgggttcg atccggcgga gaccagaccc
     4141 gtaatcgggc cgcaggacga gcgcggcttc gggcttacgg ccatgcgcga gcgcgtcgaa
     4201 cagattgggg ggaagctcgc cgtcgagaca gcgccggggg agggtaccgc catcgccgtg
     4261 gagttgcgtg acggggaagt tccgcgaact atcgaggagg tacattgacc gagcaacgca
     4321 ccagaatcct ggtctgcgac gatcacccgg tggtgaggtc gggcctcagg ggtatgatcc
     4381 agagccagcc ggacctggag gtcatcgccg aggccggcga cggcgcacag gctgtggcgc
     4441 tagccggccg gctccggccg gacgtggtgc tcatggacct gaggatgccg gggatggacg
     4501 gcgtggcggc gacggcctcc atcaaagaga ggcatcccgc catccacatc cttatcctta
     4561 ccacctacga cacagacgcc gatatcctgc gcgccctgga taagggggcg accgggttcc
     4621 tgctgaagga cgagcgcgag gacaggctct tcgacgccat aagagaggcc gcggacggca
     4681 ggtccaccct ggcgccaggc gtggcttcac gcctggtaga gaggttcagg ggaaacgcag
     4741 attcacacgt tagcgagaga gagatagaga tcctcatgct cgtcgcgcaa ggatggagca
     4801 accgggagat cgcccgcgag ctgcacataa gcgactcgac ggtcaaggcg cacatgctcc
     4861 acatcttcga caaactcggc gtgaccgacc gaaccgcggc cgtcaccacg gccctcagac
     4921 agggcatcat aaggctggaa ccgtgatacg aggcaacccg atttcgggct cgtggtcgag
     4981 aaatcggctc cgagatagat gcgctgcttc tgaggaagtc aggcaacagg ctacaggcaa
     5041 ctctcctcta gcctgttgct tgatgccttt ctattcgggt ttgctatagg gcaacacgat
     5101 tctgcgggcg aggataccgc cgatgcagat tgccggggcg ccaaacagcc acagggacga
     5161 gggaccaccg gtctcgggca gcttctcgag atc
//
LOCUS       DBNGVD010000206         2421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2594896, whole genome shotgun sequence.
ACCESSION   DBNGVD010000206 DBNGVD010000000
VERSION     DBNGVD010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2594896"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="AC16CM_05735"
     CDS             complement(<1..552)
                     /locus_tag="AC16CM_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3139365.1"
                     /translation="MQRIVRWCLGNKSVVILATLLLIGSGAYATTQLNQELLPDISFP
                     IVTISTPVSGAGPDLVDEQVTQPVEDAINGVQGIESVRSTSAQGFSVVLVEFDLDQDT
                     EEAESDLQAALDGISLPSQAGEPEVESQSASQFPILSLSLAARDGNLADLTEYAENDV
                     VSAIEEVDGVSSVDLIGGAEKQIK"
     gene            complement(552..1019)
                     /locus_tag="AC16CM_05740"
     CDS             complement(552..1019)
                     /locus_tag="AC16CM_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3139366.1"
                     /translation="MDREKLVDETLMLLPTLMRLVERPSPVEFGEMAHRGLPDVQVSP
                     GHIQVLIALTRGPRSVGKLAEELGVSPPAATQLVDKLTAHGMVRRHNDPADRRVVLVD
                     YVEGMHEVARTIVEKRRRPLQAALSKMTDDEASAFVKGLKLVADSFGTAGEES"
     gene            1156..2112
                     /locus_tag="AC16CM_05745"
     CDS             1156..2112
                     /locus_tag="AC16CM_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139367.1"
                     /translation="MKLRIAVALGGGIGGLALYNRWLEALGGEIPDRLGGRRHRYRWR
                     GHDLVYSVAGEGEPLLLVHGIYAGASSFEFRKNFRELSRSFRVYALDLLGCGMSDRPD
                     RRYGTEDVTSQIEDFVREEIGGQAHLVASSLSAALVVPAAVRSPRLFRKLVLICPTGY
                     GTLDRPSGRLGDVVYGLFRTPVLGNTLYHTIVSRWGIRYYLQGMTYHDPRFVSGDLIE
                     DYYRIAHQPGAKYFVSAFVSGKLNLGVADLWSRVPHRTLICWGLEAKTAPAGQAQRFV
                     ANNPRSEPRLFKNAGLLPHDERAETFNEEVRTFLVGKSSNPS"
BASE COUNT          420 a          786 c          759 g          456 t
ORIGIN      
        1 cttgatctgc ttctccgcgc cgccgatgag gtccacgctc gagacgccat ccacctcttc
       61 tatggcggag acgacgtcgt tctcggcata ttctgtgagg tcggcgaggt tcccgtctcg
      121 ggccgccagc gagagcgaga ggatggggaa ctgcgaggcc gactggctct cgacctcggg
      181 ctcgccggcc tgcgaaggca acgagatgcc gtcgagggcg gcctggagat cgctttcggc
      241 ctcctcggtg tcctggtcga ggtcgaactc caccagcacg accgagaagc cctgcgcgga
      301 cgtggaccga acggactcga tgccctgcac cccgtttatg gcatcctcaa cgggctgggt
      361 gacctgctcg tccacgaggt cgggcccggc gcctgagaca ggggtcgaga tggttacgat
      421 ggggaacgag atgtcgggca acagctcctg gttgagttgc gtcgtcgcgt aagccccgct
      481 ccctataagc agcaaggtcg ccaggatcac gaccgacttg ttgcccaggc accagcggac
      541 gatcctctgc atcagctctc ctccccagcg gtcccgaagc tatcggctac cagcttgagc
      601 cctttaacga acgcactggc ctcatcatcg gtcatcttgc tcaaagcggc ctgcaaggga
      661 cgcctgcgct tctccacgat ggtcctggct acctcgtgca ttccctctac ataatcgacg
      721 agcacgaccc gcctgtccgc cggatcgttg tgcctgcgga ccatgccgtg cgcggtgagc
      781 ttgtccacga gctgcgtcgc ggcgggcggt gagaccccca actcctcggc cagcttgccc
      841 accgacctcg gtccccgggt cagcgcgatc agaacctgaa tgtggcccgg cgaaacctga
      901 acgtcaggta acccgcggtg cgccatctct ccgaactcca ccggactggg acgctcgacc
      961 aaccgcatca acgttggtag caacatcaac gtctcgtcga cgagcttctc ccgatccacg
     1021 tgaacgctcc tacaaaactt aagtcgctta cacaattacc tcagatgcta tcctgggtct
     1081 gtagattgtc aatagaaaaa gtggttgatg ccttccggta cgaccgcggg cgtggatagt
     1141 ttccgaggag gttcgatgaa actgaggatt gcggtggctc tcgggggtgg cataggcggc
     1201 ctcgcgctct acaatcggtg gttggaggcg ctaggcggcg agatccccga ccggctcggt
     1261 ggccggaggc accgctaccg gtggaggggg catgacctcg tctactctgt ggccggggag
     1321 ggtgagccct tgctccttgt gcacggcatc tacgcggggg cctcctcctt cgagtttcgg
     1381 aagaacttcc gggagctatc cagaagcttc agggtgtacg ccctggatct tctggggtgt
     1441 gggatgtccg acaggcccga ccgtcgttac gggacggagg atgtcacctc gcagatcgaa
     1501 gacttcgtgc gggaagagat cggtggccag gcccacctcg tcgccagctc actctccgcg
     1561 gcgctcgtgg tgccagcagc cgtgcgcagc ccacggctct tccgaaagct cgtcctcata
     1621 tgcccgaccg gctacggcac cctcgaccgt ccgtcgggga ggctgggaga tgttgtatac
     1681 ggcctcttcc ggacccccgt tctcgggaac acgctctacc acaccatcgt ctcccggtgg
     1741 ggtattcgct actacctgca gggcatgacc taccacgacc cccggttcgt gtccggggac
     1801 ttgatcgagg actactaccg gattgcccac cagccggggg cgaagtattt cgtctccgcc
     1861 ttcgtctcag gcaaactaaa cctcggggtc gccgacctgt ggtcgcgcgt gccgcaccgg
     1921 accctcatct gctggggtct cgaggcaaag acggccccag ctggtcaggc gcagcgtttc
     1981 gtggcgaaca acccccgctc cgaaccgcgc ctcttcaaga acgccggcct cctgccccac
     2041 gacgaacggg cggagacctt caacgaagag gtgagaacct tcctggtagg aaaatcgtcg
     2101 aacccctcct agcggcggag ccgaaacctg cccacccgtc gtcgaacacg tttcgggcgg
     2161 ggtcgcggcg ttcgggaaag gcggttcgta gttgggcggt tcgcgaaccg cccctacagg
     2221 ggtttggaga tcacggtgcc ggtaggccgc cgacacggga ccgtgggggt ttcgatgccg
     2281 gtgaaccttg cggggctatg ggtttgcggg ggcggctttg ttggagcgtc tctttcgcag
     2341 gtatgaggtt acgccttcca ccgccagcga gaagcccagc caggcccatc ccacgccgaa
     2401 gagtatccag agcgcggcgc c
//
LOCUS       DBNGVD010000207         6876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4234095, whole genome shotgun sequence.
ACCESSION   DBNGVD010000207 DBNGVD010000000
VERSION     DBNGVD010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4234095"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2691
                     /locus_tag="AC16CM_05750"
     CDS             <1..2691
                     /locus_tag="AC16CM_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR3139368.1"
                     /translation="LRRIEREAEAAREYREIETRYRELSLAHLYRIATRELDGLRERS
                     EEAVARVAALVESEREMREEEQGVERRLRQVEEDIGRVDLRLEALEDGAEDLRTEALR
                     LERAVVRIEATRGREGERQLALSRLRAEANRVADTVRALEYSAADLEADRAGKREEAE
                     RLRRTATLARDRSASAERENARLSREVEGLRARIAALADRGTVTVLPEVELARLGAAI
                     SDLEEVDEQGVAEVARGLRDRVGAALGRVDGLSEEVSRRRGALAAAIGRAESRVRALR
                     ESAPTSSGTRLYEVIRARPGYEVAVEAALGEYGAGVLAENLDEGMKLLSQSEPVAVRL
                     DARAMEANGDTPGKPLSACVEVTDGRYAEAVQRLLTGIYVVERPDEGANANGYVAVTR
                     DGFRLTRTGVSLRPGSGNFAREARLAAWTELMEDLKNGPGGLLYDLRDEISGLGVRLD
                     SLAGTVRDLASLAARAARARTLISREAGRRRGAAEASRKELVARERESVTLAREASEK
                     ERALAAATEAAERARGELATATAASEAARLASADAERAASELRATLAAGKKRQAEIAR
                     RLERTPARKEDLTGALASRASRLAGGAVTTVRERRNALRKVKGEAAEARREISLRQTS
                     LSRRAVELAGELATARAHAERIAEEVARAETSSAEAGEEIQSEWGATLETARQEAERH
                     GEDTDKERRALARRLKRFGDVNLLAVSQEEGLRERHQFVSAQRADAEAAATELNRIIL
                     SVDGEIEARFSETFGRVREAFGEMVPRMLAGSSGVLELSEEGVEIGLRLGRKGWRPLR
                     VLSGGERALLALSFLFSVFLSQPGSGSAAFCILDEAEAALDDLNLARFLSVVDSHRAR
                     GQYLLVTHQKRTMAAADVLYGVVQDASGATTVVSKRLQGE"
     gene            2702..3916
                     /gene="ftsY"
                     /locus_tag="AC16CM_05755"
     CDS             2702..3916
                     /gene="ftsY"
                     /locus_tag="AC16CM_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3139369.1"
                     /translation="MARSWRNFFRKSTDEEPEKGQAVASPPEGAEEGNGHRSQEVVPD
                     EEIGSAVPTAEEVQAGRKEPAPADEWEVEEPSLADETLPEPSPAEAETEAQAVAEAQE
                     SSGWLGRLRQGLSRSRASFVGQLNAAVAEFRDVEDDEFWERVEEIMIASDVGVPTTAR
                     LVAELEQEALEKNITSGTELRELMIDHATRMLEGPVELDISHTPTVILMVGVNGTGKT
                     TTIGKLSRFLPGTTMFAAGDTFRAAAIEQLQTWGERTGTPVVARERGADAAAVAHESI
                     QAARRTGTDVLLIDTAGRLHTKVNLMAELDKVQRVIQRQMPGAPHEVLMTVDATTGQN
                     GLRQAQMFKEVAGLTGIILTKLDGTAKGGIALAIANEVGVPIKLISVGEKVEDLHPFD
                     AREFAEALFGDL"
     gene            3917..5248
                     /gene="ffh"
                     /locus_tag="AC16CM_05760"
     CDS             3917..5248
                     /gene="ffh"
                     /locus_tag="AC16CM_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3139370.1"
                     /translation="MFDTLGDRLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YGVVKEFVSNVRERATGAEVSKALSPGQQVVKIVDEELANLMGGTAHKLSFASRPPTV
                     VMLAGLNGHGKTTTAGKLALFVRKLGKNPYLVACDTYRPAAIDQLQQIGRELSVPVYT
                     EGTGPRPEEIAERGIKEARDGGYDFVIVDTAGRQVVDMEMMEEIRRVRKVSRPHSVLL
                     VLDVVTGQNAVEVAQAFQEYADFDGMVLTKLDGDARGGAALSVVSVTGRPVKFASEGE
                     KMGEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDQTAAEAQGKKLMSGKFTFEDFLQ
                     QMQMIKKMGPLSGLLGMIPGLNKQLKDVEIDDRQLGRVEAMITSMTVKERRNPKLLQN
                     PSRARRIARGSGATQQDVQKLVKQFNEMQKMMRQMGKGGGMPRIPGLSGRR"
     gene            5273..5515
                     /gene="rpsP"
                     /locus_tag="AC16CM_05765"
     CDS             5273..5515
                     /gene="rpsP"
                     /locus_tag="AC16CM_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3139371.1"
                     /translation="MAVKIRLARHGSKGNPFYRIVVADSRSPRDGKFIDRIGTYDPQQ
                     EPSDIKVDEEKAREWLGKGAQPTEQVRNLLKISGVL"
     gene            5523..5762
                     /locus_tag="AC16CM_05770"
     CDS             5523..5762
                     /locus_tag="AC16CM_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3139372.1"
                     /translation="MEQLENLLAVLARSLVDEPDEVEVSGTETDSRVDLELRVAPDDM
                     GRVIGRHGRTIRAIRTVVKAASVKQGKRVSVEVPD"
     gene            5846..6400
                     /gene="rimM"
                     /locus_tag="AC16CM_05775"
     CDS             5846..6400
                     /gene="rimM"
                     /locus_tag="AC16CM_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3139373.1"
                     /translation="MKQADDSNHHETEPGEPGDVAPRPAGAVPIGVVVGTHGVRGTLR
                     VRAFGPGRHLRKGMEPVVGGIRRRISGVRETPKGFLVDVEDIGSREAARSLKGEELLI
                     GRGELDPPEEGEYYVADLVGLTAFDDGGGVVGVVIDTFETAAHEVLIVRDEGGSDLYV
                     PFTLEHVPELDPGSRRLVIRPPES"
     gene            6413..>6876
                     /locus_tag="AC16CM_05780"
     CDS             6413..>6876
                     /locus_tag="AC16CM_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3139374.1"
                     /translation="MDVFCVFPDAVRASVMVGVVGRAIERGTVGFRTFDLREHAPGGR
                     IDDMPYGGGAGMVVRVDVVARALEEVYGVPAREVREDRRVVLTEPAGRRLTQGYVDEV
                     AAGADLTVICGRYGGMDERVWTELATEAVSLGDFVLSGGEIVAAALADAAIRR"
BASE COUNT         1277 a         2005 c         2563 g         1031 t
ORIGIN      
        1 ttgcgcagga tcgagcgcga ggccgaggcc gcccgcgagt accgggagat cgaaacgcgc
       61 taccgcgagc tctccctggc ccacctctac aggatcgcga cgagggagct ggacgggttg
      121 agagaacggt ccgaggaggc cgtcgcccgg gttgccgcgc tggtcgaaag cgaacgcgag
      181 atgcgcgagg aagagcaggg cgtcgagcgg cgcctgcgac aggtcgagga agacatcggg
      241 cgggtagacc tgcggctgga ggccctcgaa gacggcgcag aggatctgcg gaccgaggcg
      301 ctacgcctgg agagggccgt ggtccgcatc gaggccactc gggggcgtga aggagagaga
      361 cagctcgctt tgtctcgcct gcgggccgag gcgaaccgcg tcgccgacac cgtgcgggca
      421 ctggaataca gcgccgccga cctcgaggcc gatcgcgcag gcaagcgcga ggaagcggag
      481 aggttacgcc ggacggccac gctggccagg gaccggagcg cctcggccga gcgcgagaac
      541 gccaggctat cccgcgaggt cgagggcctg cgggcccgca tcgccgcgct tgccgatcgt
      601 ggcacggtga ccgtgctgcc tgaagtggag ctcgctcggc tcggcgcggc catttcggac
      661 ctcgaggagg ttgatgagca gggtgttgcg gaggtggcgc gcgggttgcg tgaccgggtc
      721 ggcgccgcgc ttggtcgtgt agatgggtta tcggaggagg tcagccgtcg ccgaggcgcg
      781 ctggccgcgg ctatcgggag ggccgagtcg cgggtacgtg cgcttcggga gagtgctcct
      841 accagctcgg gcaccaggct ctacgaggtt ataagggccc gtcctggcta cgaggtcgcg
      901 gtggaagcgg cgctcgggga atacggggcc ggggtcctgg ccgagaacct ggacgagggc
      961 atgaagctac tctcgcagtc cgaaccggtc gcggtacgct tggatgcacg ggccatggag
     1021 gccaacgggg atacgcctgg gaaaccactc tccgcctgtg tcgaggtaac ggacgggaga
     1081 tacgcggaag ccgtgcagcg gctactcacg ggcatctacg tggtcgagcg tccggacgaa
     1141 ggcgcgaacg cgaatggcta cgtcgccgtc accagggacg gcttccgcct cacacgcacc
     1201 ggggttagcc taaggccagg ctcggggaac ttcgcgcgtg aagcacggct cgctgcctgg
     1261 accgagctta tggaggactt gaagaacggt ccgggtggct tgctgtatga cctcagagac
     1321 gagatatccg ggttgggggt acgccttgac tctctcgccg gaacggtcag ggacctcgct
     1381 tcgttagcgg cccgcgcggc acgagcgagg accctgatct cccgcgaagc cggccgccgc
     1441 cggggcgcgg cggaggcttc gcgcaaggag cttgtcgctc gggagagaga atccgtgacc
     1501 ctggcgcgcg aggcctccga gaaggagagg gcgctagccg cggccacgga ggcggcggag
     1561 cgggcgcggg gcgagctggc cacggccaca gcggcgtcgg aggcggcccg tctggcgtca
     1621 gccgatgccg agcgcgcggc ttcggagttg cgggccacgc tcgcggcggg caagaaacgt
     1681 caggcggaga tcgcacgccg tctcgagcgg acgcctgccc ggaaggaaga cctgaccggg
     1741 gcgcttgcaa gcagagcgtc gaggctggcg gggggggccg taaccacggt cagggagcgg
     1801 aggaacgcgc tccgaaaggt caagggggag gcagcggagg cccggcggga gatctccctc
     1861 cggcagacct cgctctcgcg gagggccgtc gagctggccg gagagctggc cacggcaagg
     1921 gcgcacgccg agagaatcgc cgaggaggtt gccagggccg agacttcctc cgccgaggcc
     1981 ggggaggaga ttcaatccga gtggggcgca acgcttgaga ccgccagaca ggaggccgaa
     2041 aggcacggtg aggacaccga caaggagcgg cgcgcgctcg cgcggcgact caagcgcttc
     2101 ggcgatgtga acctgctcgc cgtttctcag gaagagggcc tgcgcgaacg ccaccagttt
     2161 gtctcggcgc agcgggccga cgccgaggcc gcagccaccg aacttaaccg cataatacta
     2221 tcggtcgatg gggagatcga ggcgcgattt tcggagacgt tcgggcgggt cagggaggct
     2281 ttcggggaga tggtaccgcg gatgcttgcc gggtcctcgg gcgtgctgga gttatccgag
     2341 gagggtgtag agataggcct caggctcggg aggaaaggtt ggcgcccgct gcgggtgctg
     2401 tctggcgggg agcgggcgtt gctcgcgctc tctttcctgt tcagcgtttt cctcagccag
     2461 ccggggagcg gttcggcggc cttctgcatc ctggacgagg ccgaggcggc gctcgacgat
     2521 ctcaacctgg ccagatttct ctctgtggta gactctcacc gggcaagagg ccagtatctg
     2581 ctggtcaccc accagaagcg aacgatggcc gcggcggacg tgctctacgg ggtagttcaa
     2641 gacgcctccg gggccaccac cgtagtgtcc aagcgtttgc agggagagta agaagccgcc
     2701 catggcgcgt tcgtggcgta attttttcag gaagagcaca gacgaagagc cggagaaggg
     2761 ccaggcggta gcgagccctc cggaaggggc cgaggaaggc aacggccacc gctcgcagga
     2821 agtggtgccc gacgaggaaa tcgggtcggc cgtgccgacc gctgaagagg tacaggccgg
     2881 gcgaaaagag ccggctcccg cggatgagtg ggaggtggaa gagccctctc tggccgatga
     2941 aactttgccc gagccctcgc cagccgaggc tgagacggag gcccaggctg tcgctgaggc
     3001 ccaggagtcg tccgggtggt tgggcaggct caggcagggt ctcagtcgaa gccgggcctc
     3061 ctttgtgggg cagctcaacg ccgccgtcgc cgagttcagg gacgttgagg acgacgagtt
     3121 ctgggagagg gtggaggaga tcatgatcgc ctctgacgtg ggggttccca ccacggcgag
     3181 gctggtcgcc gagcttgagc aggaggcact ggagaagaac atcacaagcg gcaccgagct
     3241 acgagagctc atgatcgacc acgccacccg catgctggag ggtcccgtag agctcgacat
     3301 ctcgcacacg ccgacggtga tcctcatggt cggcgtaaac ggtacgggca agaccacgac
     3361 gattggcaag ctctcgcgct tcctgccggg gaccaccatg ttcgccgccg gcgacacctt
     3421 cagggcggcg gccatcgagc agttgcagac gtggggggag cggacgggca cccccgtcgt
     3481 ggcccgcgag cggggcgcgg atgctgccgc cgttgcccac gagtccattc aggccgccag
     3541 gcggaccggc accgacgtgt tgctcatcga cacggcgggc aggctgcaca ccaaggtcaa
     3601 cctgatggcc gagctggaca aagtgcagcg ggtcatccag cgtcagatgc caggagcgcc
     3661 gcacgaggtg ctcatgacgg tcgatgccac cacgggccag aacgggctca ggcaggcaca
     3721 gatgttcaaa gaagtagccg gtctgaccgg tatcatacta accaagctcg acggaacggc
     3781 aaaggggggc atcgcgctcg ccatcgccaa cgaggtcggg gtacctataa agctgatctc
     3841 tgtcggggag aaggttgagg atctgcaccc cttcgacgcg agagagttcg ctgaagcatt
     3901 gtttggagat ttgtagatgt tcgatactct gggcgacaga ctcaacaaag ccctcgacgg
     3961 tgtacgcggc agcggcaacc tgaccgagga ccaggtaaag aaggtaactc gtgagattcg
     4021 tctggccctt ctcgaggccg acgtgaacta cggcgtggta aaggagttcg tctccaacgt
     4081 gcgcgagcgg gccacaggcg cggaggtctc caaggccctg agcccgggcc agcaggtcgt
     4141 caagatagtg gacgaggagc ttgccaacct gatgggcgga accgcccaca agctctcttt
     4201 cgcctcgagg ccgccgacgg ttgtgatgct cgccggcctg aacggccacg gcaagaccac
     4261 gacggcgggc aagctggcat tgttcgtgcg caagctcggc aagaaccctt acctggttgc
     4321 gtgcgacacc taccgcccgg ccgccatcga ccagctgcag cagatcgggc gcgagctctc
     4381 ggtgccggtc tatacggaag gtaccggccc caggcccgag gagatcgccg agcgcggcat
     4441 caaggaggcc agggacggcg gctacgactt cgtcatcgtc gacacagcag gtcgccaggt
     4501 ggtcgatatg gagatgatgg aggagatccg gcgcgtcagg aaggtgtcga ggccgcactc
     4561 ggtgctcctg gtcctcgacg tggtaacggg ccagaacgcg gtcgaggtgg cgcaggcctt
     4621 tcaggagtac gccgacttcg acggaatggt cctgaccaag ctcgacggtg atgcccgtgg
     4681 cggcgccgcg ctctctgtgg tctcggtgac ggggcggccg gtcaagttcg cctccgaggg
     4741 cgagaagatg ggcgagttcg actacttcta cccggacagg atggcgggac gcatcctggg
     4801 gatgggcgat gtgctcacgc tcatcgagaa ggccgagcag cagatggacc agaccgcggc
     4861 cgaggcgcag ggcaagaagc tcatgagcgg caagttcacc ttcgaggact tcttgcagca
     4921 gatgcagatg ataaagaaga tgggcccgct ctccgggttg cttggtatga tccccggcct
     4981 gaacaagcaa ctcaaggacg tcgagataga cgacagacag ctcggccggg ttgaggccat
     5041 gatcacctcc atgaccgtaa aagagcgcag aaacccgaaa ttgttgcaga acccgagccg
     5101 ggcgcgtagg attgcgcggg gctctggggc gacccagcag gacgtccaga agctcgtcaa
     5161 gcagttcaac gagatgcaga agatgatgcg ccagatgggc aagggcggcg ggatgccccg
     5221 catacccggc ctgtcgggac gcaggtagag taagagttcg agaggagatg gtatggcggt
     5281 aaagatcagg ctggcgcggc acggttccaa gggaaacccc ttctaccgga tcgtggtggc
     5341 cgactcgcgc agcccgcgcg acggcaagtt tatcgacagg atcggcacct acgacccgca
     5401 gcaggagcct tcggatatca aggtggacga ggagaaggcc agggagtggc tcggcaaggg
     5461 ggcgcagccg acggagcagg tgcgcaacct gctgaagatc tccggggtac tctagagccg
     5521 gcatggagca gctagagaac ttgctcgccg tcctggcccg ctcgctcgta gacgagcccg
     5581 acgaggtcga ggtctcggga accgaaacgg actcgagggt ggacctcgag ctcagggtgg
     5641 cgcccgacga catgggacgc gtgataggtc gccatggccg caccatacgc gcgatccgca
     5701 cggtggtgaa ggcagcctcc gtgaaacagg gcaagcgggt ctccgtggaa gtccccgact
     5761 gaggtaccaa cccacggccg cgtgatacgt ccgtaggggc tggcttcaga cccgccaacc
     5821 cagggaagac ctacggtggt tgcatttgaa acaggcggac gattccaacc accacgagac
     5881 ggagcctggg gagccgggtg atgtggctcc ccggccggcc ggtgccgtgc ctattggtgt
     5941 cgtggtcgga acgcacgggg tgcgcggtac cctgagggtc cgggctttcg gccctgggcg
     6001 acacttgagg aagggtatgg agccggtggt aggtggcatc aggcgtcgta tctccggtgt
     6061 cagggagacc cccaaggggt tcctcgtcga cgtggaagac atcgggagca gggaagcggc
     6121 acgatccttg aagggcgaag agctactgat aggccgcggt gagctggatc ccccggaaga
     6181 gggagaatac tacgtggcgg acctggtcgg gttgaccgcg ttcgacgatg gcggtggggt
     6241 cgtgggcgtt gtgatcgaca ccttcgagac ggccgcccac gaggtgctga tcgtacgcga
     6301 tgaggggggt agcgatctct acgtcccctt taccctggag cacgttcccg aactggaccc
     6361 cgggtcgcga cggctcgtga tccggccccc agagagctag cctgtgcaga acatggacgt
     6421 cttctgcgtc ttccccgacg ccgtccgggc ctccgtaatg gtcggtgtcg tgggaagggc
     6481 tatcgagcgt ggcaccgtcg gcttccgcac cttcgacctg cgcgagcatg ccccgggtgg
     6541 ccgcatagac gacatgcctt acggcggtgg tgcgggcatg gtggtcaggg tcgatgtggt
     6601 ggcccgggcg ctggaggagg tctacggcgt tccggcccgc gaggtcaggg aggaccggcg
     6661 ggtcgtcctc acggagcccg cggggcgcag gctcacgcag ggctacgttg atgaggtcgc
     6721 cgcgggggct gaccttacgg tcatctgtgg acgctacggg gggatggacg agcgggtgtg
     6781 gacggagctc gcaaccgagg ccgtctcgct cggggacttc gttctgtcgg ggggtgagat
     6841 cgtggccgca gcgctggctg acgccgcaat ccggcg
//
LOCUS       DBNGVD010000208         5933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4051930, whole genome shotgun sequence.
ACCESSION   DBNGVD010000208 DBNGVD010000000
VERSION     DBNGVD010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4051930"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16CM_05785"
     CDS             complement(<1..200)
                     /locus_tag="AC16CM_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MntP/YtaF family protein"
                     /protein_id="HIR3139375.1"
                     /translation="MTLVSLLILGFILSLDNARLAVALGAFDLGWRRALRIALVFGLW
                     DGVSPLVGLLVGRYFGKEIGEVA"
     gene            complement(197..1333)
                     /gene="pqqE"
                     /locus_tag="AC16CM_05790"
     CDS             complement(197..1333)
                     /gene="pqqE"
                     /locus_tag="AC16CM_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HIR3139376.1"
                     /translation="MDRPFGMLAELTYACPLHCPYCSNPLNLADYKDELDTEEWQRVL
                     SEARALGVLQLHLSGGEPLQRRDLVEIVACARGLGFYTNLITSALGLSRRRAEELKSA
                     GLDHVQISIQAEEQSLSDRIAGTPSYERKIVAARMVKEMDWPLTMNVVIHRHNIDRMG
                     RILDLAERLGADRIELANTQYYGWARRNRAALLPTRAQLDRAEEVVREARGRLRDVME
                     IIYVIPDYYSKYPKPCMGGWGKNQLTVTPNGNVLPCPAAHTLELPRATVRESSLASIW
                     AESPLFNRFRGTDWMPDPCRSCDRREVDFGGCRCQAFQLTGDAARTDPVCHLSPDHEI
                     VAEVVRTANQEENRDEDELVPRVITVRGSNLPTADISENERSMS"
     gene            complement(1326..1607)
                     /gene="pqqD"
                     /locus_tag="AC16CM_05795"
     CDS             complement(1326..1607)
                     /gene="pqqD"
                     /locus_tag="AC16CM_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HIR3139377.1"
                     /translation="MSITVQATNRPRLARLVRLEWDNVRESHVLLAPEGVLVLNRTGA
                     TILRLCDGERTVEEIVEELRTKYDRVSGDEVGDFIGKLAARRWVVLGDG"
     gene            complement(1604..2305)
                     /gene="pqqC"
                     /locus_tag="AC16CM_05800"
     CDS             complement(1604..2305)
                     /gene="pqqC"
                     /locus_tag="AC16CM_05800"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HIR3139378.1"
                     /translation="MVVSDTEGFVDALRAQSQGYHSLHDFHVRMNEGGLDRGQIQGWT
                     ANRFYYQENIPRKDAAILSNCPDRGVRRRWIRRIHDHDGTGEEDGGIEAWLRLGEAVG
                     LTREEMWDDRHVVPGVRFAVDAYVNFARTRPWVEAVASSLTELFAPDLMAERLAAFER
                     LYTWIDPSGLAYFRSRLTQAPRDSEHALEVVMEHCRTPEAQARAVAALSFKNDVLWSM
                     LDAIDRAYPGTEERS"
     gene            complement(2308..3201)
                     /gene="pqqB"
                     /locus_tag="AC16CM_05805"
     CDS             complement(2308..3201)
                     /gene="pqqB"
                     /locus_tag="AC16CM_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HIR3139379.1"
                     /translation="MWVRVLGSAAGGGFPQWNCGCPGCRAVRAGSRPSRPRTQSSVAV
                     SADYRSWYLLNASPDIRAQIEAFPALHPKEIRDTPLRSVLLTDAELDHTLGLLLLRES
                     DDIEIHCTEAVHETLLDGTSLLQTLGAYSGVGWRAVATGKETSLGDGLTYLAFGVPTD
                     KTARFGTGADGESVVGYRISDKRTGRALVYVPGAQQLTEPVREQLRDCACLLFDGTCW
                     QDDEMVRLGIARKTARDMGHVPISGEGGSLEKLALLPIERKIYIHINNTNPVLIEDSP
                     ERRAVQERGLEVAFDGLELEI"
     gene            complement(3316..3417)
                     /gene="pqqA"
                     /locus_tag="AC16CM_05810"
     CDS             complement(3316..3417)
                     /gene="pqqA"
                     /locus_tag="AC16CM_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HIR3139380.1"
                     /translation="MEATTMEWVTPEFEEVGTSAEVTAYAGIWPDSE"
     gene            complement(3498..5186)
                     /locus_tag="AC16CM_05815"
     CDS             complement(3498..5186)
                     /locus_tag="AC16CM_05815"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR3139381.1"
                     /translation="MTQEYVPAPPAVSMQDLTAQKGSAPQVTKDVNYERILGAREKEP
                     HNWLTYYGDYDGKRYSLLDQINKDNVSKLLPAWVFQFGSQGLHAGPSTYAFEAAPIIV
                     DGVMYVSGWDGLLWAVDAKKGNLLWQYKHAIPFDVSLCCGNVNRGVAVGHGKVFMATL
                     NAHVVAIDSETGEKVWDTIHGDVRAGESATVAPLLVKDMVIVGSSGGEFGSRGHLDAY
                     NVETGERVWRCYMVPKPGEPGFETWPEEGEAWQRGGGNCWVTGTFDPETNNLYWGTGN
                     PAPDFDGEVREGDNLYTDSVVAIDVDTGEKQWHYQCTPHDLWDYDSNMECILFEKDDR
                     KLLGHFDKNGYFFILDRLNGDLVSVTPFVDRINWGEIDAEGNVTPSIYPEKEGEEVHF
                     FPGPAGAKEWTHACFNPETELFYVPVQDTGSTVARRRREFKEGIPYWGAAVTVDSSDL
                     AGGITAFDTDGKEVWRWDNQIPMCASVLTTAGGLVFAGSPQGVFHALDAETGEHLWEF
                     QCGSGHHSSPTTYSVDGRQYIAVPVGWGAWVEGFLPGLMGGPHGSAVFAFALPE"
     gene            complement(5213..5677)
                     /locus_tag="AC16CM_05820"
     CDS             complement(5213..5677)
                     /locus_tag="AC16CM_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901661 - quinone metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_3727 family PQQ-associated protein"
                     /protein_id="HIR3139382.1"
                     /translation="MATEQQELSAVVSELGLDHQNKATIGRLLNHGGLGHVDEGDDGR
                     MSATIRITEDELTYSPSILVMPHGGDLEIEFLNDDKNTHCAIIPSNGDYQWIWLPNHS
                     RGKVNINLDGPGYYWYSSNVGNDEGRGLMGAIVVMGDVPDEAKLDRPPQPRP"
BASE COUNT          974 a         1949 c         1856 g         1154 t
ORIGIN      
        1 gccacctcac cgatctcctt gccgaagtag cgtcctacca gcaaccccac cagcggcgag
       61 acgccgtccc acagaccgaa caccagcgcg atacggagcg cgcggcgcca accgaggtcg
      121 aaggcaccga gcgcgaccgc cagccgggca ttgtccaggc tgaggatgaa cccgaggatg
      181 agaagggata caagggtcat gacatgctcc tctcgttctc ggagatgtcg gcagtcggaa
      241 ggttgctccc tcgaaccgta atgacgcggg gcacgagctc gtcttcgtcc cggttctctt
      301 cctggttggc ggtcctcacg acctcggcaa cgatctcgtg gtctggcgag aggtggcaga
      361 cggggtccgt gcgcgccgcg tcgccggtta gctggaacgc ctggcagcgg caaccgccga
      421 aatctacctc gcggcggtcg caactgcggc agggatcggg catccagtcc gttccccgga
      481 aacgattgaa aaggggcgac tcggcccaga tcgaggccag agagctctcc cggacggtgg
      541 ccctggggag ctcgagggtg tgcgccgccg ggcagggcag gacgttgccg ttgggcgtta
      601 ccgttagctg gttcttgccc cagccgccca tgcacggctt ggggtacttg ctgtagtaat
      661 ctgggatgac gtagatgatc tccatcacgt ccctcaggcg cccgcgggcc tcgcgcacga
      721 cctcttcggc ccgatccaac tgggcccttg tgggcagcag ggccgcgcgg ttacgcctgg
      781 cccatccgta gtactgggtg ttggccagct cgatcctgtc ggcccccaac ctctcggcga
      841 gatccaggat cctgcccatc ctgtctatgt tgtggcggtg gatgaccacg ttcatggtga
      901 gcggccagtc catctctttg accatgcggg ccgccacaat tttacgctcg tacgaggggg
      961 tgcccgcgat tcggtccgag agcgactgct cttcggcctg gatgctgatc tgcacgtggt
     1021 caagcccggc ggacttgagt tcttcggccc gccgccgcga gagcccgagg gcgctggtga
     1081 tgaggttggt gtagaaacca agccccctgg cgcacgccac gatctccacg agatcccggc
     1141 gctggagcgg ctccccaccg gagaggtgga gctgcagaac gccgagtgcc cgcgcctcgg
     1201 agagtacccg ttgccactct tcggtatcta gctcgtcctt gtagtccgcc aggttcagcg
     1261 ggttggagca gtaggggcag tggagcgggc aggcgtaggt caactcagcc agcatgccga
     1321 agggcctatc catcgccgag caccacccac cgcctggcgg cgagcttgcc gatgaagtcg
     1381 cccacctcgt cgcctgacac gcggtcgtac ttggtgcgca actcctccac gatctcctcg
     1441 accgttcgct ccccgtcgca caaccgaagg atggtcgcgc cggtcctgtt caagacgagc
     1501 acgccctccg gtgcgagcag cacgtggctc tcgcgcacgt tatcccactc tagccggacc
     1561 aggcgcgcca gacgcggccg gttggtggcc tgcaccgtga tgctcatgac ctctcctccg
     1621 tccccggata ggccctgtct atggcatcca gcatactcca cagcacatcg ttcttgaagg
     1681 atagggctgc cacggcgcga gcctgtgctt ccggcgtccg gcagtgctcc atgaccacct
     1741 ccagggcgtg ttccgagtcg cgcggggcct gcgtgaggcg ggacctgaag taggcaaggc
     1801 cggaggggtc gatccaggtg tacaggcgct cgaacgcggc gaggcgttcc gccatcaggt
     1861 cgggggcgaa gagctcggtc agagaggagg ccacggcctc gacccatggg cgggtgcggg
     1921 cgaagttgac gtaggcgtct accgcgaacc gtactccggg gaccacgtgc ctgtcgtccc
     1981 acatctcctc cctcgtcagc ccgacggcct cgcccaaccg cagccaggcc tctatcccgc
     2041 catcctcctc ccctgttcca tcgtggtcgt ggatgcgccg gatccagcgc cgccgcaccc
     2101 ccctgtcggg gcagttggag aggatggcgg cgtcctttct ggggatgttc tcctggtagt
     2161 agaagcggtt ggccgtccag ccctggatct gccctcggtc gagacctccc tcgttcatcc
     2221 tgacgtggaa gtcgtgcagg ctatggtatc cctgcgactg cgcgcgcagg gcatcgacga
     2281 aaccttctgt atcggatacc accaactcta gatctccagt tctagcccat cgaaggccac
     2341 ttccaggcca cgctcctgca cggcgcgccg ctccggcgag tcctcgatca ggacggggtt
     2401 ggtgttgttg atgtggatgt agatcttgcg ttctatgggg agcagggcca gcttttcgag
     2461 gctaccgccc tcgcccgaga ttggtacgtg ccccatgtcc cgcgccgtct tccgggcgat
     2521 gccgagccgc accatctcat cgtcctgcca gcaggtcccg tcgaagagca ggcaagcaca
     2581 atcccgcaac tgctcacgca ccggttcagt caactgctgg gcacccggca cgtacaccag
     2641 ggcgcggcct gttcgcttgt ccgagatgcg gtaccccacc acgctctcgc cgtccgctcc
     2701 ggtcccgaaa cgggccgtct tgtccgttgg aacgccgaag gccaggtagg tgagaccatc
     2761 accaagcgac gtctccttgc ccgtagcaac cgctctccaa ccgacgcccg agtacgcgcc
     2821 gagcgtctgc aagagcgatg tgccgtcgag gagcgtctcg tggacagcct cggtgcaatg
     2881 tatctctatg tcgtcggatt ctcgaagcaa gagcaagccg agggtgtggt ctagctcggc
     2941 gtccgtcagc agcacgcttc gcagcggagt atccctgatc tccttcggat gaagggctgg
     3001 gaacgcctct atctgggccc ggatgtcggg agaggcgttg agcagatacc agctccggta
     3061 gtcggcgctg accgccacag acgactgtgt gcgcggccgg ctcggacgcg agccggcccg
     3121 cacggcgcgg cacccggggc aaccacaatt ccactgcgga aaccctcccc ccgcggccga
     3181 tccgagcact cgtacccaca caccagtcct ccgtaaggag ctttcagccg tcagctttca
     3241 gcggtgggct cttttggctg accgctagaa gctagaaatg gcgccggacc tgatcggccc
     3301 ggcgccgggt tctttctatt ccgaatctgg ccagataccc gcgtaggcgg ttacctcggc
     3361 gctggtccct acttcttcga actctggcgt tacccactcc atggttgttg cttccatggc
     3421 cgtcacctcc tctcgaattg gctcttctgc ttctgtaccg ggcgacttgc gccgcccggt
     3481 atggggtgtt tacctctcta ctcgggaagg gcgaacgcga agaccgcgga cccatggggg
     3541 ccgcccatga ggcccggcag gaacccttcg acccaggcgc cccagccgac ggggacggct
     3601 atgtactgcc ttccgtccac gctgtaggtc gtcgggctcg agtggtggcc gctcccgcac
     3661 tggaactccc acagatgctc accggtctcc gcgtcgagcg cgtggaacac gccctgcggc
     3721 gacccggcga acacgaggcc gcctgcggtg gtaagcacgg aggcgcacat cgggatctgg
     3781 ttgtcccacc tccacacttc cttgccatcg gtgtcgaagg cggtgatacc gccggccagg
     3841 tcggaggagt caacggtaac ggcggctccc cagtagggga tgccctcctt gaactccctg
     3901 cggcggcggg cgaccgttga gccggtgtcc tgaacgggaa cgtagaagag ctccgtctcc
     3961 gggttgaagc aggcgtgcgt ccattccttg gcgccagccg gaccggggaa gaagtggact
     4021 tcctcgccct ccttctcggg atagatgctg ggcgtaacgt tcccctcggc gtcgatctca
     4081 ccccagttga tgcggtctac gaagggggtg acgcttacca gatcaccgtt caggcggtcg
     4141 aggatgaaga agtacccgtt cttgtcgaag tggccgagca gcttgcgatc gtccttctcg
     4201 aagaggatgc actccatgtt cgagtcgtag tcccacaggt cgtgcggcgt gcactggtag
     4261 tgccactgct tttcgccggt atctacgtct atggcgacga cgctgtcggt gtatagattg
     4321 tcgccctcgc ggacttcccc gtcgaagtcg ggggccgggt tcccggttcc ccagtagagg
     4381 ttgttggtct ccgggtcgaa ggttccagtg acccagcagt tgccgccgcc gcgctgccac
     4441 gcctcgcctt cctccggcca ggtctcgaag ccgggctcgc cgggctttgg gaccatgtag
     4501 caacgccaga cgcgctcacc ggtctcgacg ttgtacgcgt cgaggtggcc gcgagaacca
     4561 aactctccgc ccgagcttcc gacgatgacc atatccttta cgagcagcgg ggccaccgta
     4621 gcgctctccc cggcgcggac gtcgccgtgg atggtatccc acaccttctc gccggtctcc
     4681 gagtcgatcg cgacgacgtg cgcgttcagg gtcgccatga agaccttgcc atgccccacg
     4741 gcgacgccgc ggtttacgtt gccgcagcag agcgagacgt cgaaggggat tgcgtgcttg
     4801 tactgccaga gcaggttgcc cttcttcgcg tcgacggccc acagcaggcc atcccagccc
     4861 gagacgtaca tgacgccgtc aacgatgatc ggggcggcct cgaaagcgta ggtcgagggc
     4921 ccggcgtgaa gaccctgcga gccgaactgg aacacccagg cgggcaggag cttgctgacg
     4981 ttgtccttgt tgatctggtc gagaaggctg tagcgcttgc cgtcgtagtc accgtagtag
     5041 gtgagccagt tgtggggctc tttctcgcgg gccccgagga tgcgctcgta gttgacgtct
     5101 ttcgttacct gcggcgcgct ccctttctgc gcggtaaggt cttgcattga gacggcgggc
     5161 ggcgccggta cgtattcctg cgtcactgat tctcctttcc tagcgaggtt tcttacggac
     5221 gcggttgcgg tggacggtcg agcttggcct catcaggcac gtcccccata acgacgatgg
     5281 cccccatcag cccccggccc tcgtcgttgc ccacgttgga gctgtaccag taataccctg
     5341 gcccatcgag gttgatgttg accttgcccc gcgaatggtt gggcagccag atccactggt
     5401 agtccccgtt gctcgggatg atcgcacagt gggtgttctt gtcgtcgttg aggaactcga
     5461 tctccaggtc gccgccatgg ggcatgacga gtatggaggg cgaatacgtc aactcgtctt
     5521 cggtgatcct gatcgtggcg ctcatgcgcc cgtcgtcacc ctcgtctacg tgcccgagcc
     5581 ccccgtggtt gagcagacgc cctatggtcg ccttgttctg atggtcaagc ccaagttcgc
     5641 ttacaaccgc tgaaagctct tgttgctcgg tcgccatcga tgctctctcc tttccccagc
     5701 tcttcaccaa gaatgctacg tctacaacct cgtaggtgcc cttggttcca ccccctaacg
     5761 cgttaccccc aggaacaata caataatata tcaacccgct tgatgcaatc gttgtgaacg
     5821 gaagagcagg caacgtgctt tttgttgctt tccagggcgt tgggcgcgtc agggacgtcg
     5881 cataccatgc aaggcatcct gagaggccac ggcgaagggt gggggttcgc agg
//
LOCUS       DBNGVD010000209         7883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2413359, whole genome shotgun sequence.
ACCESSION   DBNGVD010000209 DBNGVD010000000
VERSION     DBNGVD010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2413359"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC16CM_05825"
     CDS             complement(<1..665)
                     /locus_tag="AC16CM_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3139383.1"
                     /translation="MELRRLRYFVAVAEELSFNRAAQRLRISQPPLSNQIKRLEEELG
                     VLLFVRTNRGVRTTEAGEALLEEARRILVQLEQTTRVVQRVGHGEVGRLTLGFVPSAS
                     NEVLPPILRTFRNRFPDVELFLREMRPDQVVSRLHDKQIDAGFLFLPLDDALLNIECV
                     SREPLVLALPESHRLAAKPRVELQALAEEPFILPARYSMPGLFGQVTRACRQAGFVPR
                     AVQ"
     gene            1022..2359
                     /locus_tag="AC16CM_05830"
     CDS             1022..2359
                     /locus_tag="AC16CM_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139384.1"
                     /translation="MQRADSEDGGAGGVLTDVREEPRQLRPVVVAAAVGSFVEYYDFA
                     VYGALAAILSVVFFPSGNPTAALLSTFAIFAVAFFVRPLGGLLWGHLGDKIGRQRTLA
                     TVIILMAVGTVGIGLLPSYAAIGLAAPVLVVALRCVQGFSAGGEIMGASTFVAEYAPE
                     GRRGFLTSFIQSATTAALLAGAAIAFLLTSIFPEDAVTSWGWRIPFLLAGPIGIIGLY
                     IRLRLEDTPHFKAIQEGHEVTQNPVRETLSHGENYKQIVLAAGVGVVQFVAFYMLLTY
                     MPTYLTEELGFQSWESFLSLTIATAFLMVTIPFAGLVSDRIGRRTTLMVATATLLVLT
                     YPCFLLISQGTFLAAVVGQVLLAIPASATAANLLAAQTEMFPTRIRYTAYAIAMNIGV
                     ALFGGTAPFVATYLISVTGSNLAPAFYLMFAAALSLITALAMTETAKEPLRDV"
     gene            2514..3311
                     /locus_tag="AC16CM_05835"
                     /pseudo
     CDS             2514..3311
                     /locus_tag="AC16CM_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            3432..4478
                     /locus_tag="AC16CM_05840"
     CDS             3432..4478
                     /locus_tag="AC16CM_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3139385.1"
                     /translation="MKAFVKTGHQPGDAEIREVSVVRPGPGEVLLRVASCGVCGSDVH
                     ALRSDPGFEWVTTPVTLGHEFSGIVEEVGSGVARTSPGDRVVAVAVQGCGRCETCLSG
                     TTQLCPDRVAVGLSRDGGMAEYAIMPERHLVPVPEGLDLAVAALGEPLSVAVHAVDVR
                     AEIGPEQRVVVSGPGPIGVLCGMLARHRGAEVLLTGVGQDSESRLPAAERAGLRTANL
                     SERPLEDHLRDRFGEPAPDVWIESSGSVGALGSALESVRPGGTVGVVGLYAEELRFSP
                     TNAVRREIGLLFSYSCNYADYQTALGLLSAGAIDPNPLLSKYPLGSAQEAFEAVGRGR
                     TVKAMLLPQGDARR"
     gene            complement(4580..5209)
                     /locus_tag="AC16CM_05845"
     CDS             complement(4580..5209)
                     /locus_tag="AC16CM_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139386.1"
                     /translation="MEHWHGFETARNVAQVYETTIADTASDLSAHLVTVPARSLGGGS
                     YLFRGPDGPLETLGLLRVADVPFGGVARSLAACLVSDDVEEFLRRFDRGFEEETQHSS
                     VRLPKPFEFAEYLTFARVVPFEQPSLSADSLGNILTAQGHGEAGYVYHEATGTPLLAV
                     AIPSGMVICGSGTGLVRALDSGLRQRLVEFIKDRSGERHGGDEGVGRDG"
     gene            5467..6324
                     /locus_tag="AC16CM_05850"
     CDS             5467..6324
                     /locus_tag="AC16CM_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3139387.1"
                     /translation="MAELVELREAVAGLVSDGDTVALEGFTHLIPSAAAHEIIRQEKK
                     DLTLIRMTPDLIYDQMIGTGCARKLIFSWGGNPGVGSLHRFRDAVENGWPRRLELEEH
                     SHAGMANRYVAGASNLPFAVLRGYVGTDLPKVTSTIVPITCPFTGEELVAVPALNPDV
                     AVIHAQQADRRGNVAVWGISGIQKEAALSAQRVIVTVEEVVEEFEPRPFQIVLPAVVV
                     DAIAVEPGGSRPSYADGYYDRDNAFYEAWDGISRDRERFGEWMERHVLGTRDFAEYTR
                     SIEREESLR"
     gene            6360..7106
                     /locus_tag="AC16CM_05855"
     CDS             6360..7106
                     /locus_tag="AC16CM_05855"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3139388.1"
                     /translation="MMTVAASRELQDGAVCFVGIGLPSEATNLARATHAPNCVLIYES
                     GTVGAKPDVLPLSIGDGVLAEYADSVVSVPEIFNYWLQAGRVDVGFLGAAQMDRYGNI
                     NTTVIGPYESPKVRLPGAGGAPEIAASARDVVIILRQKPRSFVEELAFVTSVGHRHGG
                     DSRNELGYGGAGPTIVITDLGVLRPDPETRELTMTALHPGATVEEAKEATGWELKVAG
                     ELETTDPPTEGELTILRDLKARTAESRKRA"
     gene            7202..>7883
                     /locus_tag="AC16CM_05860"
     CDS             7202..>7883
                     /locus_tag="AC16CM_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3139389.1"
                     /translation="MSYGGTNEAWIVGAVRTPIGRHGGALSAVRPDDLGAVVLEALVE
                     RASVPPSEVEDVYFGCANQAGEDNRNVARMSLLLAGFPETVAGATVNRLCGSGLEAVA
                     QAARSIMLGEADVYVGGGVESMSRAPWAVPKQERAFPTGNATMYDTTLGWRMVNPKME
                     QMGHTDSLGMTAENLAQERFLLTPDEDKPDEVASAYDVSREAQDRFALRSHEKALAAT
                     DEKRFREEI"
BASE COUNT         1458 a         2459 c         2632 g         1334 t
ORIGIN      
        1 ttctgtaccg ccctcggaac gaaacccgcc tggcggcagg cccgggtaac ctgtccgaag
       61 agaccgggca tcgaatagcg cgcgggaagg atgaacggtt cttcggcgag ggcttgtagc
      121 tcgactcgcg gttttgcggc gagacgatgc gactccggca gggcaagaac cagaggttca
      181 cgcgagacgc attctatgtt gagcaaggca tcgtcgagcg ggaggaacag aaaccccgca
      241 tcgatctgct tgtcgtgcag acgtgacact acctggtctg gtcgcatctc acgtaaaaag
      301 agctcgacgt ccggaaagcg gttcctgaac gtacgcagta tgggcggtag cacctcgttg
      361 gaggccgaag gcacgaagcc caacgtgagg cggccaacct cgccatgccc cacacgctgg
      421 acgacgcggg tagtttgttc tagctgaacg aggatacggc gtgcttcttc caggagtgct
      481 tcccccgcct cggtcgtcct gacgccgcgg ttggtcctca cgaaaaggag aaccccaagc
      541 tcctcttcga gccgcttgat ctgattgctc aagggcggct gcgagatacg aagcctctgg
      601 gcggcccggt tgaagctgag ctcctcggcc accgccacga agtaacgcaa acgccgtagt
      661 tccatgttcg ggttatagca aaaacgtatg cccggtgcac taaattagta ttggacatat
      721 gacccgtgga tgataacaat agcgaccagg gaaggaaacg gaccgagatg atcaagtggg
      781 ggaaaggatt cttggagaaa gtaggccgtc ggagctaacg ccgaagacgg ggtgtccgca
      841 cgtagctcat gcgcggccag aagtacggaa agatggcctg tcggacatct cgtgtgaggc
      901 tctaggggag cctctctttg aggggtagtg tcatggaacg cccagctaca caggtgacac
      961 tccctaaacc tctgcaagtg actaatcgat cttcgtaatc ggtcttcgaa aggggagaag
     1021 tatgcaacgc gcagatagcg aagatggtgg agctggcggg gttcttaccg acgtacggga
     1081 agaaccccgc cagctgcggc cggtggtcgt cgcggccgcg gtcggcagct tcgtggagta
     1141 ttacgatttc gcggtgtacg gtgccctcgc cgcgatcctg tcggtcgtct ttttcccctc
     1201 cggaaatcct accgccgcgt tgctatccac gttcgcgata ttcgcggtgg ccttctttgt
     1261 gcggcctctc gggggtctgc tctggggcca tctcggggac aagatcgggc gccagcgcac
     1321 gctggccacc gtcatcatcc tcatggccgt gggcacggtc ggtataggat tgttgccgag
     1381 ctacgccgcg ataggcctgg cggctcccgt cctggttgtg gccctgcgat gcgtgcaagg
     1441 cttctcggcc gggggcgaga tcatgggcgc gagcactttc gttgccgaat acgcaccgga
     1501 ggggcgcagg ggcttcctga cgagcttcat acagtccgcg accacggccg cgttgctcgc
     1561 cggcgcggcg atcgcgttcc tgttgacgag catctttccg gaggacgccg tgacctcctg
     1621 gggatggcgc ataccattct tgctggcggg accgatcggc atcatcggtt tgtacatccg
     1681 gctgaggctc gaagacaccc cacatttcaa ggccattcag gagggccacg aggtcacaca
     1741 gaaccccgtg cgcgaaacgc tcagtcatgg ggagaattac aagcagatag tcctggcggc
     1801 cggggtgggc gtcgtccagt tcgtcgcctt ctacatgctg ctgacctaca tgccgaccta
     1861 cctcaccgag gagctcggct tccagagctg ggagagcttc ctctcgctaa ccatcgcgac
     1921 cgcgttcttg atggtcacca tcccgttcgc gggcctcgtc tccgaccgaa tcggcaggag
     1981 gacgacgctt atggtggcca cggcgacgct gttggtgctg acctatccct gcttcctgct
     2041 cataagccag ggcactttcc tcgccgccgt ggttggccag gtgctgctgg ccataccggc
     2101 ctccgccacc gcagcgaacc tgctcgcggc ccagaccgag atgttcccca cccgcatccg
     2161 ctacaccgcg tacgccatcg ccatgaacat cggcgtcgct ctgttcgggg gcaccgcgcc
     2221 gttcgtggcc acgtacctga tctctgtgac aggaagcaac ctggcccccg ccttctacct
     2281 gatgttcgcc gccgcgctgt cgttgatcac cgccctggca atgacggaga cggccaagga
     2341 gccgctccgc gacgtgtagc cacggaggtc tagacggagc gggcaaggag agtctaggtg
     2401 acttcgggcg aaaaggcgtt cagaccgact ggtcggaacg aacaggcaag gcatcgattc
     2461 gcgacgtgtc cgatgccttg cgcgtccccc aaaacgagat aggagggtgt cggatcgcgg
     2521 attcggtgga cgaagcggca cgcctggtcg acgcggccga agccgccaaa gtcccgctcc
     2581 tcgtgggcca ccaccgacgt cacagcccca tcctggtgaa ggcgcgccat gtagcacggg
     2641 acggcacgct cggaccgatg gtcgccgtga tgggcagcgc gcttttctac aagcccagcc
     2701 actacttcga cgaagcgccc tggcggcgcc aagccggcgg cggtcccatc ctgatcaaca
     2761 tgatccacga agtagacaac ctgcgctccc tatgcggtga catcgtggac gtgcaggcct
     2821 tctcctccaa tgcgacccgc ggcctccccg tggaaggcac cgttgcgatc aacctgcgtt
     2881 tcgcaaacgg cgccctcggc acgttcctgc tctcggacac ggcggcgtcg gtgaggagtt
     2941 gggagcagac atcgcgcgag aacaccggct acgcgcacta ccaggacgag gattgctact
     3001 tgatctccgg tacgctcggc tcgctcgcgg tgcccaccat gaggctaaaa gtctacgcgg
     3061 gagagcgttc ctggtggaag ccgttccacg aagacatcgt caacatcgaa cgcgccgacc
     3121 cgctggcgcg ccagctagag cacttctgct ccgtcatccg cggcgagacg gagcctttgg
     3181 tgagcgggcg ggacgggctc caaacgctgc gcgttacgct cgcgatacac gagggcgcac
     3241 tgaccgaaag ctccgtgcac ctgccgcttg cggagaatac cggggcaacg gtgcacaagg
     3301 aggggagttg agcgacatgc ttacgctacg aagtctcggt ttgcggcaaa gtaatcgtcg
     3361 accgacgagt tgaacaggtg agtcttggca gggtatttga agcagttcct cggaatccca
     3421 ggggtgcgta gatgaaggct ttcgtaaaga cgggtcatca acccggagac gctgagatca
     3481 gggaggtatc ggtcgtccga cccggccccg gtgaagttct gctgcgtgtg gcgtcctgcg
     3541 gtgtctgcgg cagcgacgtc cacgctctca gatcggaccc cggcttcgag tgggtcacga
     3601 cgccggtgac gctcggtcac gagttctcgg gcatcgtcga ggaggtcgga tccggcgttg
     3661 cgcggacgag ccccggagat cgggtggtcg cggtcgcggt acagggttgc ggccgctgcg
     3721 agacttgcct gtccggcacg acccagttgt gtcccgatcg cgtggccgtg ggcctctccc
     3781 gcgatggggg gatggcggag tacgccatta tgcccgagcg gcacctcgta cccgtccccg
     3841 aaggcctcga cctcgcggtg gcggccctgg gcgagccgct gtccgtggcc gtgcacgcgg
     3901 tcgatgttcg ggcggagata ggccccgaac agcgggtggt ggtgtcgggg ccggggccca
     3961 tcggggttct gtgcgggatg ctagccaggc acaggggggc ggaagtgctg ctcaccgggg
     4021 tcgggcaaga ttccgagtca cgcctcccgg cggcggagcg cgcggggctc aggaccgcca
     4081 acctgagcga gcggcccctg gaagatcatt tgcgggaccg ctttggggag cccgctcccg
     4141 acgtctggat cgagagttcc ggatcggtgg gggcgctcgg ctccgctttg gagtccgtgc
     4201 gtccgggtgg gacggtcggc gtggtcggcc tgtacgccga ggagttgcgg ttctccccca
     4261 ccaacgcggt acgcagagag atcggcttgt tgttcagcta ctcctgcaat tacgcggact
     4321 accagacggc cctgggcctg ctgtcagccg gcgccataga ccccaatccc ctgctctcga
     4381 agtatccact cgggagcgca caggaggcct tcgaagcggt tggccggggc cggaccgtga
     4441 aggcgatgct cctccctcaa ggagatgcaa ggagatgaat tcaggggcga agaaccgacc
     4501 agatctcatg aggagggcat cgatcgtgtc acgatgccgt acgaaacgcc tccggatcgg
     4561 gcgagcgcca gccgagaggc tatccgtctc gcccgacgcc ctcgtctccg ccgtggcgtt
     4621 cgccggagcg gtccttgatg aactcgacga gccgttgcct gagacccgaa tcgagggccc
     4681 ttaccaaccc cgtgcccgag ccgcaaatta ccattcccga cgggatcgca acggccaaaa
     4741 gcggggttcc ggtcgcttcg tggtacacgt agcccgcttc gccatggccc tgcgccgtca
     4801 ggatgttgcc cagggaatcg gccgacagcg agggttgctc gaacgggacg accctcgcga
     4861 aggtcaggta ctcggcgaac tcgaagggct tgggcaaccg cacgctggag tgctgggtct
     4921 cctcctcgaa gccccggtcg aacctccgca ggaactcctc gacgtcgtcg gagacgaggc
     4981 acgcggccag gctgcgggcc acgccgccga acggcacgtc cgcgacgcgc aacagcccca
     5041 gcgtttcgag cgggccgtcc gggccccgaa acaggtaaga tcccccaccc agcgatcgcg
     5101 cggggaccgt gacgaggtgg gccgaaaggt cagacgcggt gtccgcgatc gtggtctcgt
     5161 atacctgcgc gacgttgcgg gccgtctcga aaccgtgcca gtgctccata cccgcagttt
     5221 acctattatc ctcgcgccgt gcgctggcgt cacatcaccg aggcatatcc gaggcattcg
     5281 tttggtattg gacacaaatc gagatagcga ccagaataac gaaggggaca agaaggtgga
     5341 acgcggaggg gcaccaagca tcaacggccg atcggatcat tgggagagcc gaagccgaga
     5401 cgcttcgatc ccccgcttgg ggttgacgga aggaggacgc gacgagcatg cgtgtaaggg
     5461 gtcgacatgg ctgagttggt agagttgcgg gaggcggtcg ccgggttggt aagcgacggc
     5521 gacacggtcg ctttggaggg cttcactcac ttgatcccga gcgcggccgc acacgagatc
     5581 atcaggcagg agaaaaaaga cctcaccctg atacggatga cgccggacct gatctacgac
     5641 cagatgatcg ggacgggttg cgcccggaag ctgatcttct cctggggcgg aaaccccggc
     5701 gtggggtccc tccaccgctt ccgcgacgcc gtggagaacg gctggccgcg ccgattggag
     5761 ttggaggagc actcccacgc cggcatggcc aaccgctacg tcgccggggc ttcgaaccta
     5821 cctttcgccg tcttgagggg ctacgtcggc accgaccttc cgaaggttac ctcgactatc
     5881 gtccccatca cttgcccctt cacgggcgag gaactcgtgg ccgttccggc gctgaacccg
     5941 gacgtcgcgg tgatccacgc ccagcaggcc gacagacgcg gcaacgtggc cgtctggggc
     6001 ataagcggca tccagaagga ggcggcgctt tcggcgcagc gcgtgatcgt cacggtcgag
     6061 gaggtggtcg aggagttcga gcccaggccg ttccagatcg tgcttccggc cgtggtcgtc
     6121 gacgccatag cggtcgagcc gggcggttcg cgcccgtcat acgccgacgg ctactacgac
     6181 cgcgacaacg ccttctacga ggcttgggac gggataagcc gcgaccggga acggtttggg
     6241 gagtggatgg agcgccacgt gctcggcacg cgagacttcg ccgagtacac gaggagcatc
     6301 gagagagagg agtcactcag gtgacagacg ccgcggcacg ggagtacacc accgacgaga
     6361 tgatgaccgt ggccgcttcg cgcgagctcc aggacggagc ggtgtgcttc gtgggcatag
     6421 gcctgccgag cgaggcgacg aacctggcgc gggccaccca cgcccccaat tgcgtcctga
     6481 tctacgagtc ggggaccgta ggcgccaagc ccgacgtcct tccgctctcc atcggggacg
     6541 gggtgcttgc cgagtacgcc gactcggtcg tctcggtgcc cgagatcttc aactactggc
     6601 tgcaggccgg gcgcgtggac gtcggtttcc tcggggccgc ccagatggac cgctacggga
     6661 acatcaacac cacggtcatc ggtccttacg agagccccaa ggtgcgtctc cccggcgctg
     6721 gcggggctcc cgagatcgcg gcctcggcga gggatgtggt catcatcctg agacagaagc
     6781 cccgctcttt cgtcgaggaa ctcgccttcg tcacctcggt cgggcaccgc cacggcggcg
     6841 actcccgcaa cgagctcgga tacgggggcg caggtccgac aatagttatc accgaccttg
     6901 gcgtcttgag gcccgatccc gagaccaggg agctcacgat gaccgccctg cacccgggcg
     6961 ccaccgtgga ggaggcaaag gaggctacgg gttgggagct caaagttgcg ggagagctgg
     7021 agacgaccga tccacctacg gagggggagc ttacgatcct gcgcgacctc aaggccagga
     7081 ccgcggaatc acggaagagg gcctaagtgg agcacgaaag attcccttgc ggctcatcag
     7141 tggtcagtga tgcagtagag agtcacgcca gaaaacctag acccaggatg ggaggagtag
     7201 catgagctac ggtgggacca acgaagcctg gatcgtggga gcggtccgca ccccgatagg
     7261 ccgccacggc ggcgccttga gcgccgtccg tcccgacgac ctgggcgccg tcgtccttga
     7321 agccttggtg gagcgtgcca gcgtaccgcc ttcggaggtc gaggacgtat atttcggatg
     7381 cgccaaccag gccggcgagg acaaccgcaa cgtcgccagg atgagcctgc tcctggccgg
     7441 cttcccggag acggtcgcgg gcgccacggt aaaccgattg tgcggctcgg gcctggaggc
     7501 cgtggcccag gccgcccgct cgatcatgct cggcgaggcc gacgtctacg tgggaggcgg
     7561 ggtggagtcg atgagccgcg ccccgtgggc cgttcccaag caggagcggg ccttcccgac
     7621 aggcaacgcc acgatgtacg acaccacttt aggctggcgc atggtcaacc cgaagatgga
     7681 gcagatgggc cacaccgact ctttaggcat gaccgcggag aacctcgccc aggagcggtt
     7741 cctgctcacg cccgacgagg acaagcccga cgaggtggcc tcagcctacg acgtctccag
     7801 ggaggcccag gaccgcttcg ccctcaggag ccacgagaag gccctcgccg cgacggacga
     7861 aaagcgcttt cgcgaagaga tcg
//
LOCUS       DBNGVD010000210         6666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_409171, whole genome shotgun sequence.
ACCESSION   DBNGVD010000210 DBNGVD010000000
VERSION     DBNGVD010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_409171"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16CM_05865"
     CDS             <1..291
                     /locus_tag="AC16CM_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3139390.1"
                     /translation="PFVAALEFASGKTASVVGKPERDFFRLALEDMGLEAGQVAMVGD
                     DAEADVLGARRAGLMGIQVRTGKWQADAAGADLIIDSVADLPEGLQTVGGEG"
     gene            288..686
                     /locus_tag="AC16CM_05870"
     CDS             288..686
                     /locus_tag="AC16CM_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3139391.1"
                     /translation="MKVQDIPVETSGHYQLVTITGEVERAVREAGVEEGICVIASTHT
                     TAAVTVNEDYDRDVPTDLAAACRAFLDVLDVRFEHAEGNSDSHFLTSLFGQSQTLLVR
                     GGTLDLGRWQGIFLAEFDGPRRRTVRVAVL"
     gene            808..2199
                     /locus_tag="AC16CM_05875"
     CDS             808..2199
                     /locus_tag="AC16CM_05875"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3139392.1"
                     /translation="MNGLVKGGLALGGLALGIGAIRRALNPTPRYLSWEKPPYNEFEK
                     KVLVLGGGFGGYTAAMDLCKMVEDRDDVGVLVIARDNFFTFWPMVPGIVSSDIDARNV
                     AQPLRRSLITSGASFRRANVTSVDSERKIVKAEGGIEFPYDQLIISLGGMPNFFGIPG
                     VAEYSMTMRGVGDAERVRNRVIERFEEVSLIRDEVPDSKLTFVVIGGGATGVEVASEI
                     HTLVHEHLAPDYPNIDPHRVRIYLLEALPNILLELDPALRRAARTRLANERIEVMVNT
                     MAEEITDECVKLKGGSQIDTENVIWTAGNRPNQTVQDFGLPFDERTGIKVDLTLKVEG
                     EDDIWAIGDCAAVQDVRQEEGKIVPPTAQAAIQQGHVVARNVIKNIDGEQDLREFEYK
                     PLGQLVELGSDFAVNEVMGVRFTGVIAAVFWRMAYLVRLRSPQSRVRIAADWIFGLFF
                     RPAVTQIRGSSDE"
     gene            2296..2865
                     /gene="pgsA"
                     /locus_tag="AC16CM_05880"
     CDS             2296..2865
                     /gene="pgsA"
                     /locus_tag="AC16CM_05880"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3139393.1"
                     /translation="MSLANQLTLLRLVAVVPVMLALALPFEWARYVAVVLYVGAILTD
                     YFDGIVARRNNEVTGFGKLMDSIADKALIVSLFFALVGEGSMPAWMAAVMVIREFAVT
                     GLRMVALEAGEVIAANSWGKAKMNAQSLAVFILLLGKPGAEGWQGALSQIGWWVMLVA
                     VVLTLLSGWSYLKDTPRILSITSKRDQRL"
     gene            2982..3827
                     /locus_tag="AC16CM_05885"
     CDS             2982..3827
                     /locus_tag="AC16CM_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139394.1"
                     /translation="MEGGNDKRLRPAEVEMILRRAAELNARRWTRDAEETPSVSPEVL
                     VQVAAAAGLPEQDVRRAIWDMSSEKTAEPQSYAHRFYGPARLRAVRDVERPAENVGED
                     LEGLLRLDQGLKLRRKTELGSMWDPGDQLGVVRRTLDFSGDRTLLKAGSVEMRAEEVG
                     DSRCEASLTADYSNQRGEYLSLAGILAATLAVLFVLAGMQDPLFLLGVVPALAAPVFG
                     FRYAYGRGCREIRRSLDDLLDAVEMDRPKPDDAQDRPDRPQGPIQGLEPIPRFAPNKN
                     KDKKT"
     gene            3997..6201
                     /locus_tag="AC16CM_05890"
     CDS             3997..6201
                     /locus_tag="AC16CM_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR3139395.1"
                     /translation="MPLNVLKEIFGFDSFRPGQEEVIRAVLEGKDTLAVMPTGGGKSL
                     CYQIPALMQESLTVIVSPLISLMKDQVDSLLQSSVADAATLHSGLSAEERWEVERRMR
                     TGEVKMLYVAPERLRSLEFVLSLRRAGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVR
                     DLGNPPVLALTATATPRVRKDILRSLKMRSPEVVVTSFNRPNLTYRVIPAREKKHKLP
                     RILDVIRTSPPPGIVYGTTRKECEELAAELRRAGVDAAAYHAGMGSARRASVQESFMT
                     DELDVVVATIAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGRDGINSECVVLYRG
                     ADLGRRKRLVTLGKAGEGEVSSFFSALAGVESGGRVNVPPGSLSALGGVDPESAGIVM
                     GSLEESGLISRGYDLWAEVEVHRIEEEPEGLREEVSAVHAALPGSGTVGLPALARKVG
                     LRPAVVQGAVYRMMVDDIVEAVPRGSLVDVRLKAPSLDADSRRTIAARLKNRANTAYA
                     QIRDVESFADLRTCRREHLLRHFGDEEEVAPCGGCDVCLGEVEEPARTGRAGVSIGTP
                     LVSAFAHLAPEDDGSGVDPELFERLRTWRSEQARQQQVPAYVVLHNSHLEEISTHKPH
                     NIQELGAIKGIGLRRAARYGEDLLALIHGEDVGVGDESDGTSETAGTDVTADGGDGTE
                     SNGFRVHLESAERLLGEGRGADAVPELGRAIELGGEEARREVNRLLGKSRVG"
     gene            6224..>6666
                     /locus_tag="AC16CM_05895"
     CDS             6224..>6666
                     /locus_tag="AC16CM_05895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3139396.1"
                     /translation="MRLSGKRVAILVAEGFEDLEYWVTVMRLQEEGAEVVSVGPNTDP
                     VTGKNALEAQADTTADGVDATELDGVVIPGGWAPDKLRRYPDILDLVRTVHERGRPVG
                     IICHGGLVAISAGIVSGARATGSLGIKDDLENAGAIWVDEAAFREG"
BASE COUNT         1212 a         2026 c         2296 g         1132 t
ORIGIN      
        1 cccttcgtgg cggcactcga gttcgcaagc gggaagaccg cgtccgtcgt gggcaagccc
       61 gagcgggatt tcttccgact ggcgctggag gacatgggtc tcgaggccgg gcaggtggcc
      121 atggttggcg atgatgccga ggctgatgtc cttggggcta ggagggccgg tctcatgggt
      181 atacaggtaa ggaccggaaa atggcaggcc gacgccgcgg gggccgacct cataatagat
      241 agcgtcgctg atttgccgga aggtcttcaa acagtgggag gagaaggatg aaagttcagg
      301 atatacccgt cgagaccagc gggcactacc agttggtcac cataaccggc gaggtagagc
      361 gtgccgtgcg ggaggccggt gtggaagaag gcatctgcgt catcgcttca acgcacacca
      421 ccgcagcggt caccgtcaac gaggattacg acagggacgt gcccacggac cttgcggcgg
      481 cgtgccgggc cttcctcgac gttctggacg tgcgcttcga gcacgccgag ggtaacagcg
      541 actcgcactt tctgaccagc ctcttcggac agtcgcagac gctgctcgta agaggcggga
      601 cgctcgatct cgggcgctgg cagggcatct ttctggccga attcgacggc ccgcgcagga
      661 ggacggtcag ggtcgccgtc ttgtaggtca cctgtaatcc cgattgttta ggcgggcgtc
      721 aacattcggt aagataggat cgtgaatctt ggggtcgcgg gctcagatgt cgtggccggt
      781 cgaaaagggt ctttgtggga ggcttgtatg aacggattgg tcaagggtgg gctggcgcta
      841 ggtgggttgg ccctcggcat cggggcgata cggcgggcgc taaacccgac gcccaggtat
      901 ctctcctggg agaaaccacc atacaatgag ttcgagaaga aggttctcgt gctaggcggc
      961 ggcttcggcg ggtacacagc ggcgatggac ctgtgcaaga tggtcgagga tcgcgacgac
     1021 gtcggggtcc tggtgatagc ccgcgacaac ttcttcacct tctggcccat ggttccaggt
     1081 atcgtcagca gcgacataga cgcccgcaac gtcgcgcagc cccttcggcg ctcccttatc
     1141 acctcggggg cgagcttcag gcgggcgaac gtcacaagcg tcgactccga gcgcaagata
     1201 gtcaaagccg agggcggcat cgagtttccc tacgaccagc tcataatctc ccttggcggc
     1261 atgcccaact tcttcggcat ccccggggtt gccgagtaca gcatgaccat gaggggggtc
     1321 ggggacgccg agcgcgtacg taaccgcgtc atcgagcgtt tcgaggaagt ctcgctcata
     1381 cgcgacgagg tgcccgactc caagctaacc ttcgtggtca ttggcggggg ggccaccggc
     1441 gtggaggtcg cctccgagat ccacaccctc gtgcacgagc acctcgcgcc cgattacccc
     1501 aacatcgacc cccatcgcgt caggatctac ctcctcgaag cgctgccgaa catcctgctg
     1561 gagctggatc ccgccctcag gcgcgccgcc cgcaccaggc tcgccaacga gcggatcgag
     1621 gttatggtga acacaatggc cgaggagata accgacgagt gtgtcaagct caagggtggg
     1681 agccagatag acacggagaa cgtgatctgg acggcgggca accgcccgaa ccagactgtg
     1741 caggacttcg ggctgccctt cgacgagcgg acggggatca aggtcgacct cacgctcaag
     1801 gtcgagggcg aggacgacat ctgggccata ggggactgcg ccgccgttca ggacgttcgt
     1861 caggaggaag gcaagatcgt gccgcccacc gcccaggcag ccatacagca gggccacgtg
     1921 gtggcaagga acgtgatcaa gaacatcgac ggcgagcaag acctccggga gttcgagtac
     1981 aagccgctag gacagctcgt ggagctcggg agcgacttcg ccgtcaacga ggtaatgggc
     2041 gtgaggttca cgggggtcat cgccgccgtg ttctggcgta tggcctacct ggtacggttg
     2101 cgcagcccgc agagcagagt gcggatcgcc gctgactgga tctttgggct cttcttccgt
     2161 cctgccgtta cgcagattcg gggttcatcc gacgaatagc cccgcaactt gagacacggc
     2221 cacaaagatg ctagtttgac cgggtctaat gaggaaagga cgcggcgtga caggggatag
     2281 aagaccaggg gtcacatgag cctcgcaaac cagcttaccc tgctccgact ggtggccgtc
     2341 gtccccgtga tgctggccct cgccctaccc ttcgagtggg cgcgttacgt tgcggtggtg
     2401 ttgtacgtcg gcgcgatcct gaccgactac ttcgacggta tagtggcccg ccgcaacaac
     2461 gaggtaacgg gcttcggcaa gctgatggac tcgatcgccg acaaggccct catcgtctcg
     2521 ctgttcttcg cgctcgtagg agagggctcc atgcccgcct ggatggcggc cgtcatggtc
     2581 ataagggagt tcgccgtgac gggcctgagg atggtcgccc tcgaggccgg ggaggtaata
     2641 gccgccaaca gttggggcaa ggccaagatg aacgcccaga gcctggccgt ctttattctc
     2701 ctgctgggca aacccggggc ggaggggtgg cagggggccc tcagccagat agggtggtgg
     2761 gtcatgctcg tcgccgtggt gctcaccctg ctctccggct ggtcctacct gaaagacacc
     2821 ccgaggatcc tctccatcac ctcgaagcgg gatcaaaggc tctagagcgg accgctcggc
     2881 cgacctcgtt gtcggctatc agccagagcc ggcaactttt tgtttttccg acggctgaaa
     2941 gctaaccgca gatagccaaa agctgtagaa tcacgcccca gatggagggc ggtaacgaca
     3001 agcgattgag gccggcggag gttgagatga tcctgcgccg cgccgccgag ttgaatgccc
     3061 gcaggtggac ccgcgatgcc gaagagaccc cttcggtatc ccccgaggtt ctggtgcagg
     3121 ttgcggcggc ggccggtctc ccggagcagg acgtgcggcg ggctatctgg gacatgtcct
     3181 cggagaagac ggccgagccc cagtcctacg cccacaggtt ctacgggccg gcccgtctca
     3241 gggccgtgcg ggacgtggag cgccccgccg aaaacgtggg tgaggacctg gaaggcctac
     3301 ttcgcctgga tcagggcctc aagctcaggc gcaagacgga gctcgggtcc atgtgggacc
     3361 ccggcgacca gctgggcgtc gtgcgcagga cactagactt ctcgggcgac aggactctgc
     3421 tcaaggccgg aagcgtggag atgcgcgctg aagaggtggg ggattcgagg tgtgaggcta
     3481 gcctgacggc cgactactcc aaccagcgtg gtgagtatct ctcgctggcc ggcatcctgg
     3541 cggccaccct ggcggtactc ttcgtacttg ccggcatgca ggacccgctc ttcctgctcg
     3601 gggtcgtccc cgcgctcgcg gcaccggtct tcggtttcag gtacgcctat ggccgggggt
     3661 gccgggagat caggcgttcc ctcgacgacc tgctcgatgc cgtggagatg gacaggccaa
     3721 agccggacga cgcccaggac cgccccgacc gtcctcaagg ccccatacag ggcctcgaac
     3781 ccatcccaag gttcgccccc aacaagaaca aggacaagaa gacgtagcgt aggggctggt
     3841 tcgaaaccag tccaatgtag gggcagttag cgaactgccc tagagggccc ttcgggtccg
     3901 ggccgaaacc caccctacgt tgttcgaaca cgctggcatc atggctgtga taccttcctc
     3961 tttgtctaag taggggtcat cccttatatt tatccagtgc cgctcaacgt cttaaaggag
     4021 attttcggct tcgactcgtt caggccgggc caggaagagg tgatccgggc cgtcctggaa
     4081 ggcaaggaca ccctggccgt gatgccaacg gggggtggca agagcctgtg ctaccagata
     4141 cccgcgctga tgcaggagag cctgacggtg atcgtctcgc ccctcatctc cttgatgaag
     4201 gaccaggtcg actcgttgct gcagagctcg gttgccgacg ccgcgacgct ccactccggg
     4261 ctctcggccg aggagcgctg ggaggtggaa cgcaggatgc gcacgggcga ggtcaagatg
     4321 ctctacgtcg cgccggagcg cctgagatcc ctggagttcg ttctctccct ccgacgcgcc
     4381 ggggtcggtt tgttcgtggt tgacgaggcc cactgcatct cggagtgggg gcacaacttc
     4441 cgccccgact acctgtttct gcctcgtgcc gtgagggacc ttggcaaccc gccggtgctc
     4501 gccctcaccg ccaccgcgac gccccgggtc cgcaaggaca tactgcgctc gctcaagatg
     4561 cgctcgcccg aggtggtcgt cacaagcttc aaccgaccga accttaccta cagggtcatc
     4621 cccgccagag agaagaagca caagctgccg cgtatccttg acgtgatccg cacgtcgccg
     4681 ccccccggca tcgtctacgg cacaacccgc aaggagtgcg aggagctggc tgccgagctc
     4741 cggcgcgccg gggtggacgc cgccgcttac cacgccggaa tgggctcggc ccggagggcc
     4801 tccgtgcagg agagcttcat gaccgacgag ctggacgtgg tcgtcgcgac catagccttc
     4861 gggatgggtg tcgacaagcc caacgtcagg ttcgtgatcc actccagcgt gcccggaagc
     4921 ctcccagcct acatccagga gtcgggtagg gcgggaaggg acgggataaa ctccgagtgc
     4981 gtggtcctgt accgtggggc ggacctagga cgacgcaagc ggctcgtcac gctcggcaag
     5041 gccggtgagg gcgaggtctc ctccttcttc tcggcgctgg ccggggtcga gagcggcggg
     5101 cgggtcaacg taccacctgg ctcgctctcg gcgcttggcg gcgtcgaccc cgagtctgct
     5161 ggcatcgtga tgggcagcct ggaggagtcc ggcctgatct cccgcggata cgacctgtgg
     5221 gccgaggtgg aggtccaccg cattgaggag gaaccggagg ggttgcgcga ggaggtgagc
     5281 gccgtgcacg ccgccctgcc aggctcagga actgtcgggc tgccggcgct cgcgcgcaag
     5341 gtcggcttgc gccccgccgt ggtccagggg gctgtgtacc gaatgatggt cgacgacatt
     5401 gtggaggccg tcccccgtgg ttcactggta gacgtgaggc tcaaggcccc gtcgctcgac
     5461 gctgacagcc gtaggaccat cgcggccagg ctcaagaacc gggccaacac ggcctacgcc
     5521 cagattcggg acgtggagtc cttcgccgac ctgaggacct gccgtcgcga gcacctcctc
     5581 aggcacttcg gcgacgagga ggaggtggcc ccgtgcggcg gctgcgacgt ctgcctcggc
     5641 gaggtggaag agccggccag gaccggtcgc gccggggttt ccatcggaac accgctcgtg
     5701 tccgcgttcg cccatcttgc gcctgaggac gacggttcgg gggtggaccc cgagctcttc
     5761 gagaggttga ggacctggcg gagcgagcag gccagacagc agcaggtgcc ggcctacgtc
     5821 gtgctccaca actcgcacct ggaggagatc tccacccaca agccgcacaa catacaagag
     5881 ctcggcgcca tcaagggcat cggtcttcgc cgcgcggccc gctacggcga agacctgctg
     5941 gccttgatcc acggcgaaga tgtcggggtc ggcgacgaat ccgacggtac gtcggaaact
     6001 gccggcacgg acgttacggc cgatggtggg gatggcacgg aatcgaacgg cttccgggtg
     6061 cacctggagt cggcggagcg gctcctcggg gagggccgtg gtgctgatgc cgtgcccgag
     6121 ctgggtcgcg ccatcgagct cgggggtgaa gaggcgaggc gcgaggtgaa tcggttgttg
     6181 ggcaagagca gggtcggatg atagcggggg gagaggagtc ctgatgcggt tgagcggcaa
     6241 gcgggtggca attctggtgg cagagggttt tgaggacctg gagtactggg ttacggtgat
     6301 gcgcctgcaa gaggaggggg ccgaagttgt ctcagtcggg ccgaacaccg accccgtcac
     6361 cggcaagaac gccctggagg cccaggccga cactacggcg gacggcgttg acgccacgga
     6421 gctggacggt gtcgtgatcc ctggcggctg ggctcccgac aagctgaggc gctaccccga
     6481 tatcctggac ctcgtcagga cggtccacga gcggggccga ccggtcggca tcatctgcca
     6541 cggcgggctc gtcgccatct ccgccggcat agtatcgggc gccagggcga cggggtcgct
     6601 gggcatcaag gacgacctgg agaacgccgg ggccatctgg gtggacgagg ccgccttcag
     6661 ggaagg
//
LOCUS       DBNGVD010000211         5091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1866018, whole genome shotgun sequence.
ACCESSION   DBNGVD010000211 DBNGVD010000000
VERSION     DBNGVD010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1866018"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..538)
                     /locus_tag="AC16CM_05900"
     CDS             complement(125..538)
                     /locus_tag="AC16CM_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cif family virulence factor"
                     /protein_id="HIR3139397.1"
                     /translation="MIHKKTSGRLDFAALREAIEQKDPDALLAFYAQGAELLVANAAF
                     PDSRAFELRGRAQIERYLRAVCDQEVGRAVRGEAVYGLRSIAFVEACRYSEGAPVYVH
                     TTLEIEGGLIVRQVDVVKHPGADDPTGSWAPDAGK"
     gene            complement(772..3105)
                     /locus_tag="AC16CM_05905"
     CDS             complement(772..3105)
                     /locus_tag="AC16CM_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3139398.1"
                     /translation="MTSEPGRRQTNLPLELSSFIGREKEIAGARKMLEEGRLLTLTGP
                     GGAGKTRLALAIASETAGAFEDGVWLVELAPLSDPELVPQAVASVVGVRESPGKALAD
                     TLAEHLGTRDLLLLLDNCEHLADACVSLADRLLRRCPNLRILATSREALGIAGESLFD
                     VPPLSLPDPHRGPTAGSLPEYEAVRLFVERARAVRPGFALTEENSLAIAHVCYRLDGI
                     PLALELAAARAKVLSVEQISERLDDSFALLTGGGRTRIPHHKTLRAAMDWSYDLLTGQ
                     EQTFLARLSVFAGGFALEAAEAVGTGEDIDRYEALDLLASLVDRSLVLVAEHDGEARY
                     RLLETVRQYGRGKLEGLGEADQTLRRHAGFYLALAESVDLMSREQAVWLERLEIEHDN
                     FRAALVLSLGEDAASKEDVRSGLRLAVALWPFWYTRGYLSEGRGWLESAVARGGSEDS
                     SAKAAALNGAGYITLFQGDYGAARALLEQSLALYRELGDDEGVASSLIYLGFVAVLSL
                     QDLETVPTLEEEAVGLRPRLADPLVLANLHLLSGLVALNEGDLERAVVLHRESLAMFR
                     DIHDARGMGHCFNNLGFLALLQDNHEEAAALLRENLRLARQSGYKLAIQYSFLGLGFV
                     ADSWDDPLRAARLWGATETMEETFGIEITPIARSAMNFEARVSLARSNAGETAFETAR
                     SEGRAMSPEQAIEYALQEPEPPESVEPSPAYPLGLSAREVEVLKLAAEGLTNAQIASK
                     LFISPRTVNAHLGSIYHKIGSSTRAQAARFAAEQGLL"
     gene            3411..4580
                     /locus_tag="AC16CM_05910"
     CDS             3411..4580
                     /locus_tag="AC16CM_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139399.1"
                     /translation="MSATVAGRGTGMKRKQTLGPPGHENARMSLRAAALLARAVCVLS
                     LALAGAGLLLLAANRIYLGVPVFEEWVEDTVIAVGFSTVGAVIAHRFPSRNPIGWLFC
                     ALGLVAAVLLFCGEYAVYAMVAGPWSLPGGEAAAWIVTWLWVVHSGLFAFLGLLFPDG
                     RLPTARWRYFAWTVVVVVVFGTVTTAFSPGHIDSLSLISNPLGIEGAPDLHNAVEVFV
                     FALTLAAAASVLVRLYRADGVERLQIKWFAYSASVLAVGALISWVVSDAVDARWLRWD
                     VGFVVTVTGLAGLPVALGIAILRYRLHDIDLIVNQALVYAALTAILAGVFEITLVIAQ
                     HVLLTFTHVEDSRLAYFATAMVMAAMFEPLKRRIDIFVEGYLLRKDDEDGEQTVL"
     gene            complement(4662..>5091)
                     /locus_tag="AC16CM_05915"
     CDS             complement(4662..>5091)
                     /locus_tag="AC16CM_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3139400.1"
                     /translation="EAARLWGAAESLRESLNISLAPIERSYYDPPIEAARLRVDDATW
                     AAARAEGRAMSTEQAVEHALQEQMPEEPTIPDDYPAGLSAREVEVLSLLATGLTNAQI
                     ASKLFISPRTVNAHLGSVYHKIGSSTRAEAARFAAEQGLL"
BASE COUNT          827 a         1641 c         1639 g          984 t
ORIGIN      
        1 caccaccgcc agcagcgccg ccaacgtaaa cgtcttgctc tcatctagta ggccgatcct
       61 gctctggggg ttggtatgga tggtcatgtg tacggtcctt tctctcattc tctccatgcc
      121 gcgctcactt gcccgcatca ggtgcccaac tacctgtggg gtcgtctgcg ccggggtgct
      181 tgaccacgtc cacctggcgt acgatcagcc ccccttctat ctccagcgtg gtgtggacgt
      241 agactggggc tccctccgag tagcggcatg cctccacgaa ggcgatgctc ctgaggccgt
      301 agacggcctc acccctcacc gcgcggccca cctcctggtc acagaccgcc ctgaggtagc
      361 gttctatctg cgccctgccc ctgagctcga aggctctgct gtcgggaaaa gcggcgttcg
      421 ctacgagtag ctcggcgccc tgggcgtaga aggcgagcag ggcatccggg tccttttgct
      481 cgatggcctc gcgcagcgcc gcaaagtcca gcctccctga ggttttcttg tggatcatgt
      541 gccttctcct ccttgacgcc gcgttcctgt atgcggatgc agcgtaagac cgggaggaga
      601 gggctacatc gtcaggggta cctattcggg gtacctattt gctctaggag ggtaggtagc
      661 aggactaggt atggtctctg gtgttgcggt gttcgaaagc agtgagcctg gggagcggta
      721 ggcttctggc aacaggcaac ggacgattat cctgagacct gtagcctatc ctcagagaag
      781 gccttgctct gctgcgaatc gggctgcctg ggcgcgggtg gaagagccta tcttgtggta
      841 gatggagccg aggtgggcgt ttacggtacg ggggcttatg aagagtttgc tagctatctg
      901 ggcgtttgtg agtccttctg cggcgagttt gagcacctcg acctcgcggg cgctcaggcc
      961 gagggggtag gcgggagagg gctcgacgct ctcgggcggc tctggttcct ggagggcgta
     1021 ctcgatagcc tgttccgggg acatggcgcg tccttctgac cgggctgttt cgaaggccgt
     1081 ctcgcccgcg ttcgagcgcg ccaggctcac gcgggcctcg aagttcatcg cggagcgggc
     1141 gatgggggtg atctctatgc cgaacgtttc ctccatggtc tctgttgctc cccacaagcg
     1201 cgccgcgcgg agcgggtcat cccacgagtc ggccacgaaa ccgagcccca gaaacgagta
     1261 ctggatagcc agtttgtagc ctgactggcg tgccagacgc aggttttccc gcagcaaagc
     1321 cgccgcttcc tcgtggttgt cctggagcag cgccaggaac ccgaggttgt tgaagcagtg
     1381 ccccatcccc cgcgcgtcgt gtatatccct gaacatcgcc aggctctccc ggtgcaggac
     1441 taccgcccgc tccaggtcgc cctcgttcag ggcgaccagc cccgacaaca ggtgcaggtt
     1501 ggccaacacc agtgggtctg ccagccgcgg cctgagccca acggcctctt cctcaagggt
     1561 tggaacagtt tcgaggtctt gcaggctcaa gaccgccacg aacccgaggt agatgagcga
     1621 agacgcgacg ccttcgtcgt ccccgagctc ccggtagagg gccaggctct gttccaggag
     1681 cgctctggct gccccgtaat ccccctgaaa gagggttata tagccggcgc cgttcagggc
     1741 cgcagccttc gcggatgagt cttcggaacc gccccgcgcg acggcgctct caagccaccc
     1801 acggccctcg ctcaggtacc cacgcgtgta ccagaaaggc cacaacgcga ccgccagtcg
     1861 caatcccgat ctgacgtctt ccttggatgc cgcatcttct ccgagcgaca ggacgagcgc
     1921 ggctctgaag ttgtcgtgtt cgatctccag gcgctccagc cacaccgcct gctccctcga
     1981 catcaggtcg acgctctcgg ccagcgccag atagaacccg gcgtgtcgtc gcagggtctg
     2041 atcggcctcg cccaacccct ccagttttcc acgtccgtac tggcgcaccg tctccagaag
     2101 cctgtaacgc gcttcgccgt cgtgttcggc aacgagcacc agcgatctgt ctaccaggga
     2161 cgccagcaga tccagtgcct cgtatctgtc gatgtcctct cccgtaccga ccgcttcggc
     2221 ggcttcgagc gcgaaaccgc cggcgaatac ggagaggcgt gcgagaaagg tttgctcctg
     2281 accggttagc aggtcgtagc tccagtccat cgccgcccgg agggtcttgt gatggggtat
     2341 cctcgtccgg ccgccgccgg taagcagggc gaaggagtcg tcgagcctct cggagatctg
     2401 ctctaccgac agcactttcg ccctcgcggc ggcaagctcc agggcaaggg gtatgccgtc
     2461 caggcggtag cagacgtggg ctatggccag ggagttctct tcggtgagcg cgaagcccgg
     2521 cctgaccgcc ctcgcccgct ccacgaacag cctcaccgcc tcatactccg gcagacttcc
     2581 cgccgtcggg cctcggtggg ggtctggtag ggagagcggt ggaacgtcga aaagtgactc
     2641 gccggcgata ccaagagcct cacggctcgt ggccaggatg cgcaggttcg gacaacggcg
     2701 cagcaagcgg tccgcgagcg agacgcaggc atccgcgaga tgctcgcagt tgtccaggag
     2761 caggagcaga tcgcgcgtcc cgaggtgctc ggcgagcgtg tccgcgaggg ccttgccggg
     2821 cgactcccgc acccccacaa cagaggcgac ggcctggggg acgagctccg ggtctgaaag
     2881 cggcgccagt tccacgagcc agaccccatc ctcgaaggcc ccagcggtct ccgacgctat
     2941 cgcgagagcc aagcgtgtct tgcccgcgcc gcccggcccg gtcagggtca agagccggcc
     3001 ctcctccagc attttcctgg cccctgcaat ctccttttcc cgccctatga agcttgaaag
     3061 ctcgagcggt aggtttgtct gcctgcgtcc gggttcggag gtcatgaggg acgcattggc
     3121 gggtcgacca tcgggtccgt ctcttcaggc atcgtcgctt cgttcacgtg gccggagcct
     3181 ccgcctgtgt ttccgacgag aggcagaggc acgtccgtaa gcccaccggg catctttccc
     3241 gtgacaggac cgcgcatcat catgaaatta tagccaggcg gccatggtac ggaccaggcg
     3301 ccccaaatag gtaaccagaa taggtacccc aaataggtac ccctgacgat gccggcgtct
     3361 caccgagcgg cgatgctgta gttggaagaa aagcggtggt ggaggagacg atgagcgcga
     3421 cggtagcggg acgggggacc ggaatgaaga gaaagcagac gctcggccca cccggacacg
     3481 aaaacgccag gatgagcctc cgagccgccg ctttgctcgc tcgtgcggtg tgcgtcctct
     3541 ccctggcgct cgcgggcgcg gggctgcttc tactagccgc caaccggata tacctcggcg
     3601 ttcccgtctt cgaggagtgg gtcgaggaca ccgtgatcgc ggtcggcttc tcgacggtag
     3661 gcgcggtgat cgcccaccgc ttcccctccc gcaaccccat aggttggctc ttctgcgccc
     3721 tcggcctcgt cgcggcggtg ctgctgttct gcggcgagta cgccgtctac gcgatggtgg
     3781 cgggtccgtg gtcgttgccc gggggcgagg ccgctgcctg gatagtcacc tggttgtggg
     3841 tcgtgcactc gggcctcttc gcgtttctcg ggctactctt tcccgacggc cggctgccga
     3901 cggcccgctg gcgctacttc gcgtggacgg tcgtcgtggt cgtggtgttc gggaccgtca
     3961 cgaccgcctt ctcaccaggc cacatagaca gcctcagcct catcagcaac cccctcggta
     4021 tcgagggggc cccggacctc cacaacgccg tcgaggtgtt cgtgttcgcc ctcacgctcg
     4081 cggcggcggc ctcggtgctg gtcaggttgt acagggcaga tggcgtggag aggttgcaga
     4141 taaagtggtt cgcctattcc gcctccgtgc tcgccgttgg cgcgctcatc tcatgggtgg
     4201 tctccgacgc cgtagacgcg aggtggttgc gctgggatgt tggcttcgtc gttaccgtga
     4261 ccggcctggc gggcctgccc gtggccctcg gtatagccat cctgaggtac cgcctccacg
     4321 acatcgacct gatcgtcaac caggccctcg tctacgctgc gcttacggcg atcctggccg
     4381 gcgtcttcga gatcacgctc gtgatagcgc aacacgtgct gctcacgttc acgcacgtgg
     4441 aagactccag gctagcctac ttcgcaaccg ccatggtgat ggcggcgatg ttcgagcccc
     4501 tcaagcgccg catagatatc ttcgtggaag gctatctctt gcggaaggat gacgaagatg
     4561 gggagcaaac ggtgctctag cgggacggtt ctgcggcgac atggcacgca acgaggaaga
     4621 gcaatagacg tttctttcct cccggggccc gtagcctatc ctcagagaag gccttgctct
     4681 gctgcgaatc gggccgcctc tgcgcgggtg gatgagccta tcttgtggta gacggagccg
     4741 aggtgggcgt ttacggtacg ggggcttatg aagagtttgc tagctatctg ggcgtttgtg
     4801 aggccagtgg cgaggaggct caggacctcg acctcacggg cgctcaggcc cgccgggtag
     4861 tcatccggaa tcgtgggctc ctctggcatc tgctcctgga gggcatgctc gacagcctgc
     4921 tcggtggaca tggctctacc ctctgcccgg gccgccgccc acgtcgcatc gtcgacccgc
     4981 aaacgtgccg cctctatggg agggtcgtaa taagaacgtt ctatgggcgc gagggagatg
     5041 ttcagggact cgcgcagtga ctccgccgcg ccccatagcc gtgccgcctc g
//
LOCUS       DBNGVD010000212         6391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2413652, whole genome shotgun sequence.
ACCESSION   DBNGVD010000212 DBNGVD010000000
VERSION     DBNGVD010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2413652"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1283)
                     /locus_tag="AC16CM_05920"
     CDS             complement(3..1283)
                     /locus_tag="AC16CM_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139401.1"
                     /translation="MSASAPTQATPRRLLLLAARVAWVAIAAITLLGAFASSVPARYA
                     ELAHPSENVGEAIAELGLSATAYALYNVALDTIFVSVFAMIAIIIFWRRSGDPIALLV
                     ATMLVVWGPLNGLFVLAPGSTDGMYGPLQAILGSVLTYLGYITWMLFFYLFPSGRFVP
                     GWTRWLALLYGVFFFGLWTFTPYGPESWPPPLFGAAVLMVWGSFLVAQVYRYARVSSP
                     VERQQTKWVVFGTSVALVGVLATIFTLGAAVDLRPDQVAERMLSMFLMDAFMLALPIS
                     IGIAVLRSRLFEIDTIINRTLVYGSLTLMLAAVYFAGVVGTQYVFRAVTGQERLPQLA
                     VVVSTLAIAALFDTLRRRLQSFIDRRFYRSKYDAAKTLEAFSSKLRNETDLISLSDDL
                     VAVVTETMRPAHATLWLHPSAGSSRRSLEKEQIR"
     gene            complement(1280..2611)
                     /locus_tag="AC16CM_05925"
     CDS             complement(1280..2611)
                     /locus_tag="AC16CM_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139402.1"
                     /translation="MSTRASSHLATIRGRLLLLARAVWIVVAVTVLAIMVFSVPSSYE
                     YYSEVCTATPGVCSGPVIGQTTPVQATPEGLQALQDAGLSVRFYAISNVVIDKVFQLV
                     WFAVGALIFWRRSDDRMALLTSLFLVTFGTVTVDPAAAISLVSFQPAWWLPVRGVELV
                     GYVCSALFFFLFPGGRFVPSWTRWLAVAFVALNLFMSPFAGSYASGSALETISYLVFV
                     AGVASLVWAQVYRYRRVSSPAQRQQTRWVVFGTTLGIAGTFPTRLPVDLSLVDGNTPL
                     MLLLLEAGFSLSFLLLPLSIGVAVLRSHLFDVDVLINRTIVYLVLSAALVGTYFGGIV
                     LLQRAFLSLTGQERLPQLAVVASTLVIAALFDPLRRRIQSFIDRRFYRKKYDARKALE
                     GFSAKLRNETDLDALSEDLVSAVRETVQPAHASLWLRPRAEANRGMRERAV"
     gene            complement(2853..3281)
                     /locus_tag="AC16CM_05930"
     CDS             complement(2853..3281)
                     /locus_tag="AC16CM_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3139403.1"
                     /translation="MSAEGCLIRVGEVAERLGVSPRTVKYYEELGLVEPGERSAGGFR
                     LYGGREIERLERILQMKSIGYSLAAIRELLAARDRAQEADRVTVLGTAAEHLREREQE
                     ASELVLKIREDLRRAESLREELGRDIALCERRMRELGGPG"
     gene            3448..4659
                     /locus_tag="AC16CM_05935"
     CDS             3448..4659
                     /locus_tag="AC16CM_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139404.1"
                     /translation="MRSAAEGNEATGGGRDGGEVEGSLWRQPRSVWAVGFACVVAFMG
                     LGLVDPILPAIARQLDATPSQVELVFTSYFLVTGVAMLITGFVSSRVGHKRTLLVGLA
                     MIVVSSALAGLADGISGIVGFRAGWGLGNALFIATALAVIVEVSRAGTEKAVILYEAA
                     LGLGISVGPLLGGLLGGISWRGPFLGVAVLMSIGFFAILVLLPPIPMAEHRSSILEPI
                     RALGHPGLLTLGLTALCYNFGFFTMLAYTPFPLGLDAHGLGLVFFGWGVMLAITSVFL
                     APRIQARFGTLPTMYAMLSLVGLDLALMGAGVDSRVVLVIAVIIGGAFLGVNNTLITT
                     AVMQSSPVDRPVASAAYSFIRFVGGAAAPFLAGKLAESFAPGLPFYVGAAAVFGSIVV
                     LFSGRAYLSKV"
     gene            4842..5657
                     /locus_tag="AC16CM_05940"
     CDS             4842..5657
                     /locus_tag="AC16CM_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3139405.1"
                     /translation="MTKKSGSVGFSIGEVSQAVGLAPQTLRLWERERLIKPRRTDRGY
                     RVYTEPDVKRLREIKQLRKVEGLNFAAIRKQLGDAPETASTNGSAFGGSSEAPGERLR
                     RLRIKAHKTLKEVSAATGLSISFISALERGGSGASVASLRLLAGAYGVNMREVFGADL
                     EENSPLVRGTERPVMQWDNGVRFEEMATGEKVMDPSFIRVPPGAGSEGFYSHNGEEFI
                     YVISGRLFVELKDQGKFEVKSGDTLYFPSTTPHRWWSEDEPVEAVYVNTPPTF"
     gene            5669..>6391
                     /locus_tag="AC16CM_05945"
     CDS             5669..>6391
                     /locus_tag="AC16CM_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3139406.1"
                     /translation="MQQDGHLDALLSIPALFGAMVSPDGEWVAWSWARLGPAADVFAA
                     PTDGSRPPLRLTQTAEGDTEIVSWAPDGESVLVSQDNDGDERARLFRIRLAEPGKMEP
                     LTQANPNYFTRGGQLHPNGRWLVYAANLDARSGEETEADRLYRHDLETGERKVLAAPA
                     KGSISDPELNRAGTHVLYSRNDLDPAGQQTWLVDIEGHTDREILNFGPTVKVSACWLH
                     DGRRVLFVVEAESHRKLGVWDEG"
BASE COUNT         1217 a         2037 c         2040 g         1097 t
ORIGIN      
        1 gctcaccgga tctgctcctt ctccaggctc cgtctcgaag acccggcaga aggatgtagc
       61 cacaatgttg catgcgccgg ccgcatcgtc tccgtgacca ccgcgaccag atcgtcgctg
      121 agggagatca ggtccgtctc gtttctcaac ttcgaggaga aggcttctag cgtcttggcc
      181 gcgtcgtact tgctacggta gaaacggcgg tcgataaagg attggagacg tcggcgcagt
      241 gtgtcgaaca gggcggctat ggccagcgtg gagacaacca cggcaagctg ggggagtcgc
      301 tcctggccgg tcacggcgcg aaagacgtac tgggtcccaa ccacgccagc gaagtacacg
      361 gccgccagca tcagcgtgag cgagccgtag acgagcgtgc ggtttatgat cgtatctatc
      421 tcgaacaggc gcgaacgcag taccgcgatg cctatggaga tagggagtgc cagcatgaag
      481 gcatccatga gaaacatgct gagcatcctc tctgccacct ggtcgggacg aaggtccacc
      541 gcggccccga gggtgaatat ggtggcgagc acgccaacca gcgccacgga ggtcccgaac
      601 accacccact ttgtctgctg gcgctctacc ggcgaggaga cgcgcgcgta gcggtagacc
      661 tgcgccacca ggaagctgcc ccacaccata aggacggcgg caccgaagag cggtggtggc
      721 cagctctccg ggccatacgg cgtaaaggtc cagaggccga agaagaaaac gccgtagagg
      781 agcgccagcc agcgcgtcca gcctggtacg aagcggccgc tcgggaagag atagaagaag
      841 agcatccacg ttatatatcc gaggtaggtg agcacgctgc ccagaatcgc ttgcaaggga
      901 ccgtacatcc cgtcggtcga gcccggcgcc agcacgaaca gcccgttgag gggcccccac
      961 accaccagca tcgtcgcaac gagcagcgcg attggatcgc cggagcggcg ccagaagatg
     1021 atgatggcga tcatggcgaa gacggagacg aagatcgtgt caagtgcgac gttgtagagg
     1081 gcgtatgcgg tcgcggagag cccgagctcg gcgatggcct ctcctacgtt ctcggaggga
     1141 tgggcgagtt cggcataacg agcagggacg ctgcttgcga acgcccccag aagcgtgatc
     1201 gccgcgatcg ccacccatgc caccctggcc gcaagaagca agagccttcg tggcgttgcc
     1261 tgtgtcgggg cgctcgcgct cataccgctc gctctctcat gccccggttg gcttcagcac
     1321 gcgggcgtag ccacagcgag gcgtgtgcgg gctggaccgt ctccctgacc gccgacacca
     1381 ggtcttcgct aagggcatcg agatctgtct cgtttcggag cttcgccgag aagccttcca
     1441 gcgcctttcg ggcgtcgtat ttcttgcggt agaagcgccg gtcgataaag gattggatgc
     1501 gacgtctcag cgggtcgaac agggccgcga tcacgagggt cgaggcgacg acggcaagct
     1561 gtgggagcct ctcctgaccg gtgaggctga ggaaggccct ttgcaacaga acgatgcccc
     1621 cgaagtaggt ccctactagc gccgcgctca ggacgaggta gacgatggtg cggttgatca
     1681 ggacgtccac gtcgaagagg tgcgagcgca acacggccac gccgatggac aacgggagga
     1741 gcaagaagga caacgagaag cctgcttcga gaagcagcag catgaggggc gtgttgccgt
     1801 ctacgaggga gagatccacc ggcagccgcg tggggaaggt tccagcgatg cccagggtcg
     1861 tgccgaacac cacccacctt gtctgctgcc gttgcgctgg agaagaaacc ctgcggtaac
     1921 gatagacctg tgcccagacc aggctcgcta cgcccgccac gaacaccagg tacgagatcg
     1981 tttccagggc ggacccgctt gcatacgatc ccgcaaaagg gctcatgaac aaattaagag
     2041 caacgaacgc gacggcgagc cagcgtgtcc agcttggcac gaaccgtccg ccgggaaata
     2101 ggaagaagaa cagcgcgctg catacgtacc ccaccaactc cacgccacgg accggcaacc
     2161 accaggctgg ttggaaggag accagggaga tcgcggccgc ggggtcgacc gtaaccgtcc
     2221 cgaacgtcac cagaaacaac gaggtcagca gcgccatccg gtcatcggag cggcgccaga
     2281 agatgagcgc tccaaccgca aaccacacca gctggaagac tttgtcaatg acgacgttcg
     2341 agatcgcgta gaagcgcacc gacaaccccg catcctgcaa cgcctgcaag ccctctggcg
     2401 tggcctggac gggcgtggtc tggccaatca ccggcccgga acacaccccg ggcgtggcgg
     2461 tgcagacttc agaatagtat tcgtaactgc tcggaacgct gaagaccata atggcaagca
     2521 ccgtaactgc caccacaatc cacaccgcgc gggccagcaa cagcagacgc ccacggatgg
     2581 tagcgaggtg tgacgaggcg cgggtgctca tcccaccgcg accccgaaaa acccggaaag
     2641 tcctatcagc ctcgtacctt cggagaccgt atgccgcgac ctcccgcggc acgccgcgat
     2701 cgtgcccggc ctgatcacct cgttatggag catcgtcatc gccctgttcg gatcacccgt
     2761 aactccttct ggaactattt ctagcggtcg ggcgtctgct aaccgttacg gcgaccgtta
     2821 cggaagcgga cctcggagaa agtttcgggc ggtcaccccg ggccgccgag ttctcgcatg
     2881 cgcctctcgc acagcgcgat gtcgcgcccg agttcctcac ggagtgactc cgcgcgccgg
     2941 aggtcctcgc ggattttgag caccagctcc gaagcctcct gctcgcgctc tctcaggtgc
     3001 tcggcagccg tcccgagcac cgtaaccctg tccgcctcct gggccctatc ccttgcagcg
     3061 agtagctccc gaattgcagc cagcgaatag ccgatgctct tcatctggag gatgcgctcc
     3121 agccgctcta tctcccgccc cccgtagagc ctgaacccac cggcagaacg ttccccaggc
     3181 tcgacgaggc ccaactcctc gtagtacttc acggtgcgcg ggctcacccc cagacgctcc
     3241 gcaacctcac ccaccctgat caaacacccc tcagcgctca ccaagaaacc tctcttcccg
     3301 atagaccaag cttaaccgat ccactcccca cgtgaacgac ctaacgatca aactatacgt
     3361 gtacgtacag gtatattaag atatgcgggt tgatggaggg cgaggcgggc ccctggttct
     3421 tttcgaagag aggagtgatg aggagctttg cgatctgcgg ctgagggcaa tgaggcaacc
     3481 ggcggtggcc gggatggcgg cgaggtagag ggtagtttgt ggcggcagcc caggtcggtg
     3541 tgggccgtgg gcttcgcctg tgtggtggct ttcatgggcc tgggtctggt cgaccccata
     3601 ttgccggcga tcgcccggca gctcgacgcc acgccgagcc aggtggagct ggtctttacc
     3661 agctactttc tcgtgaccgg ggtggcgatg ctcatcacgg gcttcgtctc cagccgggtc
     3721 gggcacaagc gcacgctgct ggtgggcctg gccatgatcg tggtctccag cgcgctcgcg
     3781 gggttggctg atggcatctc cggcatcgtg ggtttcaggg ccggatgggg gcttggcaac
     3841 gcgctgttta tcgccacggc gcttgccgtt atcgtcgagg tctcccgggc ggggaccgag
     3901 aaagcggtga tcctctacga ggccgcgctc gggttgggaa tctccgtcgg gccgttgctc
     3961 ggtgggctgc tcggtggcat cagctggcgg gggccgttcc tgggcgtcgc cgtgctcatg
     4021 tccatcgggt tcttcgccat actcgtcctg ctcccaccca tccccatggc cgagcacagg
     4081 agctcgatcc tggagcccat ccgggcgctc ggccatcccg gactcctgac gctgggcctg
     4141 acggcactct gctataactt cggcttcttc accatgcttg cctacactcc cttcccgctt
     4201 ggcctcgacg cgcacgggct cgggctcgtg ttcttcggct ggggggtcat gctggcgatc
     4261 acctctgttt ttctggcccc gaggattcag gcccgcttcg gaacgctgcc cacgatgtac
     4321 gccatgctct cgctcgtcgg gctcgacctc gcgctcatgg gtgccggcgt cgactcgcgg
     4381 gtcgtgctcg tgatcgccgt catcatcggg ggggcctttc taggcgtgaa caacaccttg
     4441 atcaccaccg ccgtcatgca atcctcgccc gtcgaccgtc cggtggcctc ggccgcctac
     4501 agcttcatcc ggttcgtggg cggcgccgcc gcacccttcc tggcgggcaa actggccgaa
     4561 tctttcgcac ctggcctccc cttctacgtc ggggccgcgg ccgtgttcgg cagcatcgtc
     4621 gttctcttct ccggacgcgc gtacctgagc aaggtgtaga ccaaccaccg tcagatccct
     4681 tcgggccaca aagatacgac gtttgcgtga tgtaccgttg cgggcggggc gtgtacgcta
     4741 tcccctgcca agcgcggagg atgcttgctt ttgtaggtaa aacattacaa taatggttca
     4801 agcgcatagg gattggcatt gcgggccaga cgggagatgc attgacgaag aagagcggtt
     4861 cggtggggtt ctccatcggc gaagtttcac aggcggtggg gcttgcgcca cagaccctca
     4921 ggctatggga gagagaacgc ctgatcaaac cccgcaggac ggatcggggc tacagggtct
     4981 acaccgaacc cgacgtgaaa cgcctgcgcg agatcaaaca gctcaggaag gtcgaggggc
     5041 tgaacttcgc ggcgatccgc aagcagctcg gtgacgctcc cgagacggcc agcacgaacg
     5101 gttcggcttt cgggggttct tcggaggctc ccggagagcg gttacgacgg ctcaggatca
     5161 aggcgcacaa gacgctgaag gaggtttcgg ctgccacggg gctctcgatc tcttttatct
     5221 cggcgctcga gcgtgggggc tccggggcct ccgtcgcgtc gttgcgcctc ctggcgggcg
     5281 cctacggggt gaacatgcgc gaggtgttcg gcgccgacct ggaggagaac tccccgctgg
     5341 tgcggggcac cgagaggccc gtgatgcagt gggacaacgg cgtacgcttc gaggagatgg
     5401 cgacgggtga gaaggtgatg gatccttcat ttatccgggt gcctcctggt gcgggtagcg
     5461 aggggttcta ctcccacaac ggcgaagagt tcatctacgt gatctccggc cggctcttcg
     5521 tcgagctcaa ggaccaggga aagttcgagg taaagtctgg cgacacgctg tacttcccat
     5581 ccacaacccc gcaccgctgg tggtccgagg atgagcccgt cgaagccgtc tacgtgaaca
     5641 ccccgccgac cttctagcgc ctatccccat gcaacaggac ggacacctcg acgccctgct
     5701 ctctatcccc gcgctcttcg gggccatggt ctcgcccgac ggcgaatggg tcgcctggag
     5761 ctgggcacgc ctcggtcccg ccgccgacgt ctttgccgcg cccacggacg gctcgcggcc
     5821 tccccttcgc ctcacgcaga ccgccgaggg agataccgag atcgtctcct gggctcccga
     5881 cggcgagtcc gtgctcgtct cgcaggacaa cgacggtgac gagcgggccc gcctcttccg
     5941 gataaggctc gcggagccgg gcaagatgga gccgctgacg caggcgaacc cgaattactt
     6001 caccaggggc ggccagttgc accccaacgg gcgctggctt gtctacgccg caaacctgga
     6061 cgcaaggagc ggagaggaga cggaggccga caggctctac aggcacgacc tcgaaacagg
     6121 cgagaggaag gtgctcgccg caccagcgaa gggcagcatc tccgatccgg agttgaaccg
     6181 cgctggcacc cacgtgctgt actcgcgcaa cgatctggac cccgccggac agcaaacctg
     6241 gctggtcgac atagagggcc acacggaccg ggagatcctg aacttcggcc ccacggtcaa
     6301 ggtctctgcg tgctggctgc acgatggccg cagggtcctc ttcgtggtag aggcagaatc
     6361 ccaccgcaag ctgggcgtgt gggacgaagg g
//
LOCUS       DBNGVD010000213        12245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1684296, whole genome shotgun sequence.
ACCESSION   DBNGVD010000213 DBNGVD010000000
VERSION     DBNGVD010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1684296"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="AC16CM_05950"
     CDS             complement(<1..497)
                     /locus_tag="AC16CM_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139407.1"
                     /translation="MRLATPVLMVATLVLAFPAGAAFAARDECVPEKRCVGSGSDDHL
                     EGTKGNDRILARGGRDFVDGRGGSDKVVGGGGADGDEFVAGGLFGDSPRMSADSNRDG
                     NDTLYGGNGRDLLYGFGGSDVLIGGGGADYIMAGEYRMGRGRPGVETSKNPGVDVITA
                     GDGGD"
     gene            828..1745
                     /locus_tag="AC16CM_05955"
     CDS             828..1745
                     /locus_tag="AC16CM_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139408.1"
                     /translation="MKTQLELNIRSRGAMSGVLTMGSLDPAFAAATGLEDGEPVVVDL
                     RSVEFVEPAGLCGLAALLEYLCARCEMVDLALTGHDVPAYLERMNFFRSFEERVRTNA
                     DVAMLEERRRHNPGTLQELINFHGEEEIPGIIERISEILENQGYRLRERVAICSTLSE
                     VCANAAEHGVSSFGAYAAVQAYHHIVSGSRRRGEEVLIAIADGGVGVRETLSRNPQYA
                     EFTTTDNDALRHALEMGVSATGEIGRGGGLALVAGIAARAGGSLSLRSGAGRVTVYEN
                     RKNSRNVPNCPGTFVRVSLPRTPQENTAR"
     gene            1841..2413
                     /locus_tag="AC16CM_05960"
     CDS             1841..2413
                     /locus_tag="AC16CM_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3139409.1"
                     /translation="MVEDRAHQVFEVAGGDFGGKLMVTRRTGRAVREALEEALEGLPK
                     GGILYIDSRGVELMDYSFADEALGILASRSASGEYGDRHLVLVEEDRDLLENVEASLR
                     QRSLAMIRVDEIGAEPAIVGKVPDHLVETLQAIYDAGSITNAALAARLGLNHTACNNR
                     ATRLVKLGLIHRRTETAAPGGRQYTYERIA"
     gene            2490..3062
                     /locus_tag="AC16CM_05965"
     CDS             2490..3062
                     /locus_tag="AC16CM_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139410.1"
                     /translation="MRDEYMIERQGKRFVLYAGLLEEAHNRGLRSIETELLQVPAKEN
                     GDVAIVKAVIRTEEGKFAGIGDASPQNVTRAIAPHLIRMAETRAKARALRDAINVGVT
                     AFEELGGEEEVAAPSRSEERDGRPRAENEEGRGELPATRKQLNYLEALISDVVDDGIG
                     RFEEMVGKPVGELTREEASEWIGRLSGRAA"
     gene            3062..3265
                     /locus_tag="AC16CM_05970"
     CDS             3062..3265
                     /locus_tag="AC16CM_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139411.1"
                     /translation="MGGSVWIAALGEEVLYEEDLPSCCRRTAHGQDRSFECPVCGAMW
                     REAIPVEPEQDAFTRREERRGAA"
     gene            3292..3582
                     /locus_tag="AC16CM_05975"
     CDS             3292..3582
                     /locus_tag="AC16CM_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139412.1"
                     /translation="MGTSRKGRKKRSALRPDTALVSAAQPDQEIYIWSLPACCLRSLK
                     ETAMSEVGERRRLQLDCSCGRNWRITSSLDDRILERFVTHGGPRAGGSYPAA"
     gene            3639..4481
                     /locus_tag="AC16CM_05980"
     CDS             3639..4481
                     /locus_tag="AC16CM_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3139413.1"
                     /translation="MKEAQDVLTQYLGRVRGGRLLDADEEKALSRRAHRGDLRARQRI
                     IECNLRLVISIAKKYRGRGVAFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAVADHARTVRLPAHVVDALYRLRRAENALSLELGREATEEELVERLGVKPEEAR
                     RLREVGQPISSINARIKSEEGSEVGELLPDERSGDDYARVEAGQWERTLVEAVRSLPE
                     REARIIEMRHGLDGGETRTLREVSEELGISQERARQVEIKALRTIRTGRYASSLRHAL
                     SEAV"
     gene            complement(4658..5416)
                     /locus_tag="AC16CM_05985"
     CDS             complement(4658..5416)
                     /locus_tag="AC16CM_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139414.1"
                     /translation="MTREFEIEGSTVRYEVHGEAGVPLVLVHGWACRRADWEAIIADL
                     GNRQVVAVDLPWHGESSGGHESWDISTFADVIVELVDREHLEAPILVGHSMGGAVCVE
                     AAKRLGSTIPRVIGIDTLTYLGVYPRQEEETVRATGDALRADFTGVVRAMVESLFVDR
                     DRPGLIETVVTEMTSIPEAQGIAALEGLLRWDLDAALSGLQVPVTTFAARTLLAPEAV
                     ERYGDRMEIVPVEHGGHFYLREDPRATAALLESY"
     gene            5685..7883
                     /locus_tag="AC16CM_05990"
     CDS             5685..7883
                     /locus_tag="AC16CM_05990"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3139415.1"
                     /translation="METSRDVSDRTPELFAELGGTDNNGFPAIDSPVYHTQAGTPYLK
                     RPGVVLLAKPQTNVSGLAGFLEGFDPDLRFPEYLDDPTRLPDSSQLCKTAGQLCYASF
                     GPRRTMNENATAYIERLTSAGHGSVLEHANFNFLLYGISRSVTHELVRHRAGAAFCLA
                     GDTLVYSDHFCNGRRDGTKKRTMAQLYEMTKTSHGRSRLKLLQPRSLDEATGVFDRGR
                     VKAVVCSGVKPVFKVELEDGKSITCSRDHRFLTPDGWAPLHRITNGLEISPGGFAVHG
                     PLDTKIMVNGTAAYRDQGWLEEHYIQRNLSMTEVAGVAGASTQTVKKWLRRYGIRKPL
                     GVRTRWTFANEGKLYADRKWLEEQYVLKGLEQEAIAQLAGVSRHTIRSWVRKYGLQKP
                     MGSWSTGRIPWNKGRRYTAGWNHTPETRRSFSEQKRGDKNPQWRGGITKHAITIRRGV
                     DELRPQLLERDRYRCRLCGSRSRQLDMHHIIPVWARPDLVLDLDNIATVCRACHLTMA
                     GREMEYAERFGAAPAEEDLAKQRPRARKRLFVPRPVRIKSVAYAGEQMTYDLEMKGPN
                     HNFVANGIVTHNSQISQRYVSGSVLRFVERPEYQEDEELHRLFEERADRAAAEYDTMA
                     ERLLGRQEGGASMLTADYKTDARKKVQQTARSLLPNETEAPMVFTGNVRALRHIIEMR
                     ADAHAESEIRNLALRLFLCLRTADPILFGDYELGELPDGTYTVATKHRKA"
     gene            complement(7930..9645)
                     /gene="metG"
                     /locus_tag="AC16CM_05995"
     CDS             complement(7930..9645)
                     /gene="metG"
                     /locus_tag="AC16CM_05995"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3139416.1"
                     /translation="MSEQRRRYLVTSALPYANGPLHVGQISGAYLPADAFVRYLRARG
                     EDVVYVCGTDDHGVPITLRAEREGKSPREVVDYWWAHMQDTFERLGISFDNFSHTSSE
                     LHARNVQLFYRKLKEGGYISEAESKQMYSPTEDRFLPDRYVEGTCPVCGYKEARGDQC
                     DNCGSWYEAYELIEPHSKVTGGPVEVRETTHLYLDLPKFSERLREYVVSQDHWRTSVK
                     NFILGIIDGGLEKRPITRDIQWGVPVPEPGFEEKRFYVWFDACIGYVSSTVEWAEKVG
                     EPDRWREYWQDPDSRLIHFIGKDNTVFHTLIWPAQLMGTAEDGDEPFVLPYDVPANEF
                     MNLEVIVDGERRPMQMSTSRNLAVWLHEALERFPADPLRFYLASNLPETGDVVFSWRD
                     FQGRVNSDLIGNLGNYINRVLSFAEKYAGGEIPDPGELPEEALAVLEDFRALEARYEE
                     RMLAPRPREALGELMAMGRRANRFFDASAPWKTRKEDPEQTSLTLYVCSVLLGSIAYH
                     AAPYVPEAIERLGGFFEGPIARVSDLDGLPEAYRVTGAKPLFQRIEDDEVAAAEGLLS
                     RAVSS"
     gene            9792..10247
                     /gene="dut"
                     /locus_tag="AC16CM_06000"
     CDS             9792..10247
                     /gene="dut"
                     /locus_tag="AC16CM_06000"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3139417.1"
                     /translation="MSQDPPLSVPFRRLVIGAEPPGRAHSGDAGYDLRSVERVELSPG
                     ARALVKTGISVAIPEGYAGLVLPRSGLAVKHGISLVNTPGLIDSGYRGEILVPLINHD
                     REEAFLVERGARIAQLVLVRAATALFSEVEFLEAATDGRGEGGFGSSGS"
     gene            10295..10867
                     /locus_tag="AC16CM_06005"
     CDS             10295..10867
                     /locus_tag="AC16CM_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3139418.1"
                     /translation="MNATTHAVFGVAALAGAGLVLGTDPPAYAYPVAVAAAWIPDVDN
                     PRSTLGNGLSRVNNPLLNALSRPLSWALRTTSFVLVRTVGHRTLTHSILGLLLFSLPV
                     LLLLGGFPNLSLALVVGYASHVFADALNTRGVPLLWPVGKPFRLLPGGGVRSGGAVEV
                     AVALLTLAFTLYTLLLLYPALRGVIGLQPL"
     gene            complement(10814..11428)
                     /gene="yqeK"
                     /locus_tag="AC16CM_06010"
     CDS             complement(10814..11428)
                     /gene="yqeK"
                     /locus_tag="AC16CM_06010"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR3139419.1"
                     /translation="MGDHENANTTAENARNLARSTLSDKRYGHTLRVADTAAELARIH
                     GLDENRARLAGLIHDAAREHGYEEFLRLAEEWNLPVGEPERQSPKLLHGPVAAELARR
                     ELGVGDEGVLEAVRVHTTAKPGMEPLALALYVADKIEPARDYPSVGRLRRLAREDLRV
                     ATTESLRRAIDYNEGRGKDVHPSSRAALEWLEADHTPQGRVEQQ"
     gene            11452..>12245
                     /locus_tag="AC16CM_06015"
     CDS             11452..>12245
                     /locus_tag="AC16CM_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR3139420.1"
                     /translation="MMNGMNINSAEIVTIGTEMLLGDLVDTNTAWISQRLAELGVPIY
                     RHTTVGDNPERIVEALRQASSRSAFVITTGGLGPTTDDLTNACLATLTGREMVEYAEA
                     REHVDEMFERFGRTPTENNYKQALFPTGTELIPNPVGTAMGALVEWEETLFATLPGVP
                     VEMKTMFGETLEPLIRSRSDGAIISRTLHFAGIGESALAEKVQEFLDATDPTVAPLAG
                     QGRVRLRITTRAATEEEALDKISPVEREIVARLDDFYYGQGDETLEG"
BASE COUNT         2292 a         3968 c         3937 g         2048 t
ORIGIN      
        1 tggtctccgc catcgccggc ggtgatcacg tccaccccgg ggttcttgga ggtctcgaca
       61 ccgggtcggc ccctgcccat gcgatactcg ccggccatga tgtagtcggc cccgccgcca
      121 ccgatgagca cgtcggagcc gccgaaaccg tagagcaagt ctcggccgtt gccaccgtat
      181 agggtgtcgt tcccgtcgcg gttgctgtct gccgacatcc tcggcgagtc accaaagagg
      241 ccgccggcca cgaactcatc cccgtcggcc ccgccgccgc cgaccacctt gtccgagccg
      301 ccgcggccgt caacgaagtc ccgcccgcca cgggccagga tcctgtcgtt ccccttcgta
      361 ccttcgaggt ggtcgtcgga gccgctgcca acgcaacgct tctcgggaac acactcgtct
      421 cttgccgcga acgcggcgcc cgcgggaaac gccagaacta gcgtcgccac cattaaaacc
      481 ggagtcgcca accgcatgac cctctcctct cgccataccg cctggcccct ccaggtaagg
      541 accactatat gggaacggtg ttacggtcac gcgtccaaga tgtaacgctt cgtataagtt
      601 tcgataacgt ttggataact ggatggggaa ggggtcaggc ttcaggttac aggcatcagg
      661 aatcgcgccg aagattacct ctgccccaac gttcttcccg tcaattaaac gtcgaggctc
      721 gaagggttac tcggcagggt atcgagcttc tccagaaacc acctaaagcc tgaagactga
      781 agcctgtaac ctttctgcaa accgccttac cgactacaat aaggctgatg aagacacagc
      841 tcgaactgaa catcaggagc aggggcgcca tgagcggggt gctcaccatg gggagcctgg
      901 accccgcgtt cgccgcggcg accgggctgg aggacggcga gcctgtagtc gtcgacctgc
      961 gttcggtcga gttcgtggag ccggcggggc tctgcgggct ggccgcgcta ttggagtacc
     1021 tgtgcgcgcg ttgcgagatg gtcgacctgg cccttaccgg gcacgacgtg ccagcctacc
     1081 tggagcggat gaactttttc aggtcgttcg aggagagggt gcgcaccaac gcggacgtcg
     1141 ccatgctgga ggagcgcagg aggcacaacc cggggacgct gcaggagctc atcaactttc
     1201 acggggaaga ggagataccc ggcatcatcg agaggatctc ggagattctg gagaaccagg
     1261 gctaccgtct gcgcgaacgc gtcgccatct gctccaccct ctccgaagtc tgcgcgaacg
     1321 ccgcagaaca cggcgtctcc tccttcgggg cctacgcggc cgtccaggcc taccaccaca
     1381 tcgtcagcgg ctccaggcgc cgcggcgaag aggtcctaat agcgatagcc gacggtgggg
     1441 tgggggtgag ggagaccctc tcccgcaacc cgcagtacgc cgagtttacg accacggaca
     1501 acgacgccct gcgccacgcc ctggagatgg gcgtctcggc caccggtgag atcggccgcg
     1561 gcggtggcct cgccctcgtc gccggtatag ccgcccgcgc cggaggctcc ctctccctcc
     1621 gctccggcgc aggccgcgta accgtctacg agaaccgcaa gaactcccgc aacgtgccga
     1681 attgtcctgg tactttcgtc cgcgtctccc tgccccgcac cccccaggaa aatacagcca
     1741 gatgagccag agatttcacc agatatgcag ttgacttatc cacccccagc cggtacaatc
     1801 ctctcatatg atttcactga cgacagtgag gacagttccg gtggttgagg accgggcgca
     1861 ccaggttttc gaggttgccg gcggcgactt cggaggcaag ctaatggtga cccgtcggac
     1921 gggacgcgcg gtgcgcgagg cgctcgagga ggcattagag gggttgccca aaggcggcat
     1981 cctctacata gactcgcggg gcgtcgagct catggactac tcgttcgctg atgaggcgct
     2041 tgggatactc gcgtcgaggt ccgcgagcgg ggagtacggg gacaggcacc tcgtgctcgt
     2101 cgaggaggac cgggatctcc tggagaacgt cgaggcctcc ttgcggcagc gttcgctcgc
     2161 catgatccgg gtagacgaga tcggggccga gcctgccatc gtgggcaagg tgccagacca
     2221 cctcgtcgag acgctgcagg cgatctacga cgccggctcc atcaccaacg cggccctcgc
     2281 cgccaggctg gggctcaacc acaccgcctg caacaaccgg gcgaccaggc tcgtcaagct
     2341 cggcctgatc caccgccgca ccgagaccgc cgctcccggc ggccggcagt atacctacga
     2401 gcggatcgcc tgatgtttat ttttgatcac atcctcactg tcgtcagtga agacagcgtt
     2461 tccggcctgt ggctggagga ggtacgagca tgagggacga gtacatgatc gagcgccagg
     2521 gcaagaggtt cgtgctctac gcgggtcttc tggaggaggc gcacaaccgg gggctgagga
     2581 gcatcgagac cgagctcttg caggttcctg ccaaggagaa cggcgacgtg gccatcgtca
     2641 aggctgtcat caggacggag gagggcaagt tcgccggtat cggggacgcc tccccgcaga
     2701 acgtcaccag ggccatcgct ccgcacctga tccggatggc cgagacgcgg gccaaggcga
     2761 gggctttgcg tgacgccatc aacgtcggtg tcacggcctt cgaggagctt ggcggcgagg
     2821 aagaggtcgc tgcgccctcc aggagcgagg agcgcgacgg gcggcccagg gccgagaacg
     2881 aggaaggaag gggtgagttg cccgcgacca ggaagcagct caactacctg gaggcgctca
     2941 tctcggacgt ggtcgacgat ggtatcgggc gcttcgagga gatggttggg aagcccgtcg
     3001 gtgagctcac gcgtgaagag gccagcgagt ggatcgggcg cctctccggg agggctgcgt
     3061 agtgggtgga tccgtctgga tcgcggcgct gggcgaggag gttctctatg aggaagacct
     3121 cccgtcctgc tgccggcgca ccgcccacgg ccaggatcgc tccttcgagt gcccggtatg
     3181 cggcgcgatg tggcgggagg ccatacccgt agagcccgaa caagacgctt tcacccggcg
     3241 cgaagagcgc aggggagcgg cgtagcgaga ccggtgagag ggagacgaga cgtgggaaca
     3301 tcacgcaaag gacgcaagaa gcggagtgcc ctcaggccgg acacggccct cgtttccgcc
     3361 gcgcagccgg atcaggagat ctacatctgg tcgctgccag cctgctgcct gcgctccctc
     3421 aaggaaacgg cgatgtcgga ggtaggtgag cggaggcgcc tgcagctgga ctgctcctgt
     3481 gggaggaact ggcgcatcac gtccagcctg gatgaccgca tcctggagcg tttcgtaacg
     3541 cacgggggcc cgagagccgg aggctcctac ccggcagcat gacgaaggcg atcaaagctg
     3601 acagctgaag gctggcagct aacaagaggg gagacgcaat gaaggaagca caggacgttc
     3661 tcacgcagta tctgggaagg gtgcggggtg gcaggctgct cgacgccgac gaggagaagg
     3721 ccctctcccg gcgggcccac cggggtgacc tcagggctcg acagaggatc atcgagtgca
     3781 acctgcggtt ggtgatctcc atcgccaaga agtacagggg gcggggtgtc gccttcgagg
     3841 acctcatcca ggaggggaac gccggcctga tccgggccgt cgagaagttc gaccccgaga
     3901 tgggcaatcg cttctccacg tatgcgacgt ggtggatccg ccaggctgta acgcgggccg
     3961 tggccgacca cgccaggacc gtcaggctgc cggcgcacgt cgtggacgcc ctctaccgcc
     4021 tgcgccgcgc cgagaacgcg ctgagtctgg agcttggccg cgaggcgacc gaggaagagc
     4081 tggtagagag gctcggggtc aagcccgagg aggccaggcg gctacgtgag gtagggcagc
     4141 caatcagcag catcaacgcc aggatcaaga gcgaggaggg ctccgaggtt ggtgagctgc
     4201 tccccgacga gcgctccggc gacgattacg ccagggtgga ggccggccag tgggagcgca
     4261 cgctcgtcga ggccgtgcgg tcgctgcccg agcgggaggc caggatcatc gagatgcgcc
     4321 acggcctcga cggcggcgag acccgcacct tgcgcgaggt ctccgaagag cttggtatct
     4381 cccaggagag ggccaggcag gtggagatca aagccctcag gaccatccgc accggccgct
     4441 acgccagctc tctgcgccac gccctctccg aagccgtgta gcccggcccc agacacccag
     4501 attcccacac caggagcctc gaggaacccc tcggggctcc ccttcggcaa cccgtcgagg
     4561 accaacacta cgaagaatca cagaagcatc gatcatctgc gatgcgagtc atcagctttc
     4621 agcaacaaca aaaaagctga tcgctgaaag ctaatagcta atagctctca agcaacgccg
     4681 ccgtcgcgcg ggggtcttcg cggaggtaga agtgcccgcc gtgctctacc ggaacgattt
     4741 ccatccggtc accgtagcgt tctaccgcct cgggcgcgag cagcgtcctt gccgcgaagg
     4801 tggtgactgg aacctgaagc ccggacaacg cggcgtcgag atcccagcgc aggaggcctt
     4861 cgagggcggc gataccctgg gcctcaggga tgctggtcat ctcggtgacc acggtctcga
     4921 tcagcccggg ccggtcgcgg tccacgaaga gcgactcgac catggcccgc acgacgccgg
     4981 tgaagtccgc gcggagcgcg tcgcccgtcg cacggaccgt ctcctcttcc tggcgggggt
     5041 ataccccaag gtaggttagc gtgtcgatgc caatcacacg cgggatcgtg gaaccgagcc
     5101 gcttggccgc ctcgacacac accgcgccgc ccatggagtg gcccacgagg atcggtgcct
     5161 ccaggtgctc gcggtctacg agctcgacga tcacatcggc gaaggtgctg atgtcccagg
     5221 attcgtggcc gccggaggac tcgccgtgcc agggtagatc cacggcgacc acctgacggt
     5281 tgccgaggtc ggctataatt gcctcccagt ccgcgcgccg gcaggcccag ccgtgcacca
     5341 gcaccagcgg aacaccggcc tcgccgtgta cctcgtagcg gacggtgcta ccctcgatct
     5401 cgaactcgcg cgtcatcgca ttccccaaac ccgtagccgg cggagacgga cgcccgtcac
     5461 cattccgacc cccgcgtaat ccatcgcgaa cccttaccct gcgaccggtc gatggcggca
     5521 agtatagtcc ttcggctacc cgcttaccgc gaccacacgc cccggcatgg acacgccccc
     5581 tgggaacgag gagacgtgta gaccgcgcca ttctcccaaa cgcttggaga caacggagcc
     5641 cggatcggat aacctctgtt caatcgagcg ttgggaggaa cactttggag acgagcaggg
     5701 acgtttcgga tcgaacaccg gagctcttcg cggagcttgg aggaaccgac aacaatggct
     5761 tcccagccat cgattccccc gtgtaccaca cgcaggccgg aaccccttac ctcaaacgac
     5821 ctggtgtggt cctgctcgcg aagccgcaga ccaacgtatc cggcctcgcc gggttcctcg
     5881 aaggattcga ccccgacctc cgcttccccg agtacctcga cgaccctacc aggcttcctg
     5941 actcctccca gctctgcaag acggccggcc agctgtgcta cgccagcttc ggcccccgcc
     6001 ggacgatgaa cgagaacgcc acagcctaca tcgagcgtct gacctcggcg gggcacggaa
     6061 gcgtcctaga gcacgcgaac ttcaacttct tgctctacgg catcagccgc agcgtgaccc
     6121 acgagctggt ccggcacagg gccggggccg ctttctgtct ggctggtgac accctggttt
     6181 acagtgacca tttctgcaac ggtcggcggg atgggaccaa gaagcgcact atggcgcaac
     6241 tgtacgagat gacaaagaca tcgcatggcc gttctcgtct gaagctgcta cagccacgat
     6301 ctctagatga ggcgacaggg gttttcgacc gaggtagggt aaaggccgtc gtctgttcag
     6361 gggtcaaacc ggtattcaaa gtggaattgg aggatggcaa gagcatcacc tgctcacgag
     6421 accaccggtt cctcacccct gatggctggg cgccgctaca caggatcact aacggactcg
     6481 aaatctcgcc cggtgggttc gccgtgcacg gaccccttga tacgaagatc atggtgaacg
     6541 gcaccgctgc ctatagggat cagggatggc tcgaagagca ttacatacag cgcaatctct
     6601 ctatgaccga ggtcgccggc gttgcggggg cctcgacgca aactgttaag aaatggttgc
     6661 gcagatacgg gatcagaaaa cccctcggtg tccgtactcg ctggacattc gccaatgaag
     6721 gcaaactcta cgccgatagg aagtggctgg aagagcagta cgtgctcaaa ggtctggaac
     6781 aggaggccat agcccagctc gccggagtct ccaggcacac tattcgctct tgggtgcgta
     6841 agtacggtct gcagaaaccc atgggctcgt ggtccacggg ccggatacct tggaacaagg
     6901 gtagacgtta cacggcggga tggaaccata ctccggagac gcggcggagc ttctccgaac
     6961 aaaagagagg cgacaaaaac ccgcagtggc gcggcggaat cacgaaacac gccataacta
     7021 tccgccgagg cgtagacgaa ctgcgtccgc agctcttgga gcgtgaccgt taccgctgcc
     7081 ggttgtgcgg ttcacgctct aggcaactcg acatgcacca cataatcccc gtgtgggcta
     7141 ggcctgatct cgtgctggat ctcgacaaca ttgccaccgt ctgccgggcc tgtcatctca
     7201 cgatggccgg tcgtgagatg gagtatgccg aacggttcgg agcagcgcct gcagaagaag
     7261 acctggcaaa gcagcgaccg cgcgcaagga aacgcctgtt cgtgccgcgg cccgtccgta
     7321 tcaagagcgt tgcttacgct ggcgagcaga tgacttatga ccttgagatg aagggtccca
     7381 accataactt cgtggccaac gggatagtca cgcacaattc ccagattagc caacgctacg
     7441 ttagcggaag tgttctacgc ttcgtggagc gtcccgagta ccaggaggac gaggagttgc
     7501 accggctctt cgaggagcgt gccgaccggg cggcggccga gtacgacacg atggccgagc
     7561 gtctgctggg gcgtcaggag ggcggggcta gcatgctaac cgccgactac aagaccgacg
     7621 cgaggaagaa ggtacagcag accgcgcgct ccctgctccc caacgagacc gaggcaccca
     7681 tggtattcac gggcaacgtg cgtgccctgc gtcacatcat agagatgcgc gccgacgcgc
     7741 acgcggagag cgagatccgt aacctcgccc tgcgcctctt cctgtgcctg cgcaccgcag
     7801 accccatcct cttcggcgac tacgagctcg gagagctacc cgacggcacg tacaccgtgg
     7861 ctactaagca tcgcaaggcg tagacaggag agccaggagc cttcagcttc atttgctgac
     7921 tgctgaaggc taggagctga ccgccctaga aagtagcccc tcggccgccg ccacctcgtc
     7981 gtcctcgatc ctctggaaca gcggcttcgc gccggttacc cggtaagcct ctggcaggcc
     8041 gtcgaggtcg gagacgcggg cgatagggcc ttcgaagaac ccgccgaggc gttctatggc
     8101 ctcgggaacg tacggggcgg cgtggtaggc gatagagcct aagaggacgg agcagacgta
     8161 gagcgttagg ctggtctgtt ccgggtcctc tttgcgggtc ttccaggggg ccgaagcgtc
     8221 gaagaatcgg ttggcgcggc gtcccatcgc catgagctcc cccagcgcct cgcggggcct
     8281 tggggcgagc atccgctctt cgtagcgcgc ctcaagcgcc ctgaagtctt caaggacggc
     8341 cagcgcctcc tcgggtagtt ccccgggatc ggggatctcg ccgcctgcgt acttctcggc
     8401 gaaggagagg acgcggttga tgtagttgcc gaggttgccg atcaggtccg agttgacccg
     8461 gccctggaaa tcgcgccagg agaagaccac gtcccccgtc tcgggcaggt ttgaggcaag
     8521 gtagaagcgc agtgggtcgg ccgggaagcg ctccagggcc tcgtgcagcc agaccgcgag
     8581 gttacggctc gtggacatct gcatggggcg ccgctcgcca tcgactatga cctccaggtt
     8641 catgaactcg ttggctggca cgtcgtaggg aagaacgaac ggttcgtcgc cgtcctccgc
     8701 cgtgcccatg agctgggcgg gccagatcag ggtgtgaaag accgtgttgt ccttgccgat
     8761 gaaatggatg agcctggagt cggggtcctg ccagtactcc ctccaccggt cgggttcgcc
     8821 aaccttctcg gcccactcca ccgtggagga gacgtacccg atgcaggcgt cgaaccagac
     8881 gtagaagcgc ttctcctcga agcccggctc cgggacgggc acgccccact ggatgtccct
     8941 cgtgatgggc cgcttctcga gccccccgtc gatgatgccg aggataaagt tcttgaccga
     9001 ggtacgccag tgatcctggc tcaccacgta ttccctgagg cgctcggaga atttcggcag
     9061 gtccaggtac aggtgggtgg tctcacggac ctcgaccggc ccgcccgtca ccttggagtg
     9121 gggctcgatc agctcgtatg cctcgtacca ggagccgcag ttatcgcact gatcgcccct
     9181 ggcctccttg tagccgcaca ccgggcacgt accctcgacg tagcggtccg gcagaaaacg
     9241 gtcctccgtc gggctgtaca tctgcttgga ctccgcctcc gagatatagc cgccctcctt
     9301 cagcttgcgg tagaagagtt ggacgttccg cgcgtgcagc tcggacgagg tgtgcgagaa
     9361 gttatcgaag gagatcccga gccgctcgaa ggtatcttgc atgtgggccc accagtaatc
     9421 cacgacctcc cgcggcgatt taccctcgcg ctcggccctg agcgttattg gaacgccatg
     9481 gtcgtccgtg ccgcacacgt agaccacgtc ctctccgcgg gcacgcaggt accgcacgaa
     9541 cgcgtcagcg ggcagatagg cgcccgagat ctgcccgacg tgcaagggcc cgttggcata
     9601 aggcaacgct gatgttacga gataccgtcg tctctgctcg ctcataaata aaggatagca
     9661 ggctacaggc cacaggttac aggcctcagg agtcgagagc ggtatttcgt agcgagaatg
     9721 tcgggccggc cacgacccta cccgaaacct gatgcctgta gcccgaaacc tccagtgcta
     9781 ccattctccg gatgagccag gatcccccgc taagcgtacc ttttcgtcgt ctcgtaatcg
     9841 gggccgagcc tccaggccgg gcgcactccg gggacgctgg ctacgatctg cgctccgtgg
     9901 agcgagtaga gctttcccca ggcgccagag cactggtgaa gaccgggatc tcggtcgcca
     9961 tccccgaagg ctacgcgggg ctcgtactac cgcgcagcgg gcttgcggtg aagcatggca
    10021 tctccctcgt caacacacca gggctcatcg actccggcta ccgcggcgag atactggtgc
    10081 ccctgatcaa ccacgaccgc gaggaggctt tcctggtaga gagaggagcc aggatcgccc
    10141 agctggttct ggtacgtgcc gccaccgcgc tcttctccga ggtcgagttt ctggaggcgg
    10201 caaccgacgg ccgcggtgaa ggcgggtttg gctcctctgg gtcctagcgc tcgggtcgtc
    10261 aaacccagac ccgtccccac gtggcgcgcg tgatttgaac gcgacgaccc acgccgtatt
    10321 cggggtcgcg gcgctagcgg gcgccgggct cgtgctcgga accgacccgc cggcctacgc
    10381 gtatccggtc gcggtcgccg cggcctggat accggacgtg gacaacccgc gcagtaccct
    10441 cggaaacggc ctgagccggg tcaataatcc cctcttgaac gcgctgagcc ggccgcttag
    10501 ctgggccctc agaaccacgt ccttcgtgct ggtgaggacg gtgggccacc gcaccctgac
    10561 ccactctatc ctgggccttc tgctcttctc gctcccggtc ctgttgctcc tgggagggtt
    10621 tccgaacctt tcgctggcgc tcgtggtcgg ctacgcctcg cacgtgttcg cggacgcgct
    10681 caacacccgt ggggtgccgc tcctgtggcc ggttggaaag ccgttcaggc tgctccctgg
    10741 tgggggggtg cggtcgggtg gcgccgtcga ggtcgccgtg gccctcctga ccctcgcctt
    10801 cacgctctac acgctactgt tgctctaccc tgccctgcgg ggtgtgatcg gcctccaacc
    10861 actctagggc cgcgcggctc gacgggtgca cgtccttgcc ccgcccttcg ttgtagtcga
    10921 tggcgcgtcg cagggactcg gtggtggcca cgcggaggtc ctccctggcc agccggcgca
    10981 ggcgccccac gctcgggtag tcgcgggcgg gttcgatctt gtccgccacg tacagtgcga
    11041 gcgcgagcgg ttccatcccc ggcttcgccg tggtgtgaac ccggactgcc tccagaaccc
    11101 cttcatctcc caccccgagc tcgcgcctcg ccagctccgc cgcgaccgga ccatgcagga
    11161 gcttcgggct ctgcctctcg ggttctccca ccggaaggtt ccattcctcc gcgagcctca
    11221 ggaactcctc atacccgtgc tcgcgggccg cgtcgtgaat gaggccagcg aggcgcgccc
    11281 ggttctcgtc gaggccgtgg atgcgggcca gctcggcggc ggtatcggcc accctcaggg
    11341 tgtgaccgta gcgcttgtcc gagagcgtcg agcgggcaag gttgcgggcg ttctccgctg
    11401 tagtgttcgc gttctcgtgg tcccccatac gctggattgt agaatacggc catgatgaat
    11461 ggaatgaaca taaacagcgc tgagatcgtc accatcggaa ccgagatgct cctcggggac
    11521 ctcgttgata ccaacacggc ctggatcagc cagcgcctcg ccgagctcgg ggtacccatc
    11581 taccgccaca ccaccgtggg cgacaacccc gaaaggatcg tggaggccct gcgacaggcc
    11641 tcctcccgct ccgccttcgt gataaccacc ggtggtcttg gcccgacgac ggatgacctg
    11701 acgaacgcct gcctggcgac cctgacgggc cgcgagatgg tcgagtacgc cgaggcccgc
    11761 gagcacgtgg acgagatgtt cgagcggttc ggccgcacgc caaccgagaa caactacaag
    11821 caggcgctgt tccccacggg gacggagctc atccccaacc ccgtcggtac tgcgatgggg
    11881 gcgctcgtgg agtgggagga gacgctcttc gccaccctcc ccggggtgcc cgtcgagatg
    11941 aagaccatgt tcggggagac gctggaaccc ctgatacggt cccgcagcga cggcgccata
    12001 atttccagga cgctccactt cgccggcata ggcgagtcgg cgctcgccga gaaggtccag
    12061 gagttcctcg acgcaacgga ccccacggta gccccgctcg ccgggcaggg cagggtgcgg
    12121 ctcaggatca cgacccgcgc cgccacagag gaggaggccc tggacaagat ctcccctgtc
    12181 gagcgggaga tcgtggcccg cctcgacgac ttctactatg gccagggcga cgagaccctg
    12241 gaagg
//
LOCUS       DBNGVD010000214         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_227447, whole genome shotgun sequence.
ACCESSION   DBNGVD010000214 DBNGVD010000000
VERSION     DBNGVD010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_227447"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..424)
                     /locus_tag="AC16CM_06020"
     CDS             complement(134..424)
                     /locus_tag="AC16CM_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139421.1"
                     /translation="MHGDRIVELTLALKEADALHAVLEDLLEKSPANPTLEHTYRLLG
                     WRILAARGGSGLTGRIATLAREAESLEAYEVARDRELGPVLDGLESAENRDP"
     gene            complement(426..773)
                     /locus_tag="AC16CM_06025"
     CDS             complement(426..773)
                     /locus_tag="AC16CM_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139422.1"
                     /translation="MGNRRVALKPHASRIRHWVEEGRGDTWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHSAILDETEDGVLLKTDARDSEVFEREWRSYLGGSPDELQV
                     VITQDRIYIEKTR"
     gene            complement(870..1304)
                     /locus_tag="AC16CM_06030"
     CDS             complement(870..1304)
                     /locus_tag="AC16CM_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139423.1"
                     /translation="MALLLLAHAAGTLFMVGVIWFVQVVHYPLFARVGPSSFSAYSAR
                     HTRLTGLVVGPPMLLEATTAVALVVWSPAGVSVSLLWAGLILVAGIWLSTAVLQAPRH
                     TTLGGGFDPAVHRFLVTSNWLRTLLWSLRGLVVLCILYQAMG"
     gene            complement(1305..2303)
                     /locus_tag="AC16CM_06035"
     CDS             complement(1305..2303)
                     /locus_tag="AC16CM_06035"
                     /EC_number="1.6.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3139424.1"
                     /translation="MNESKRARTCLIVGAGISGLLAGEALQREGWEVTVLDKSRGVGG
                     RMATRRVGAGTFDHGAQFFTVRKDRFSDLVEGWLEAGAAVEWSLGFTDDGGTPNEDGH
                     PRYRGADGMTSIPKHLAGNLDVRTGERAVRADASVAGWEISCESGLRLETSALLLAMP
                     VPQALDLATSGNYGLPAGARQDLEAISYDPCLALMVLLDGSANVPEPGGMQIKGEPLD
                     WIGDNRRKGISEAPAITIHAGPEWSRAHFDDQEADITRDLISFAGDRLGTDLSSLVVE
                     TSLARWRYSWVTEAHPEPFLVSPGDPTLLFCGDGFGQPKVEGAALSGLAAADYLLG"
     gene            complement(2300..>2980)
                     /locus_tag="AC16CM_06040"
     CDS             complement(2300..>2980)
                     /locus_tag="AC16CM_06040"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3139425.1"
                     /translation="MVERSRRASGGTVGACLAALEEGFAANLAGGTHHAFTDRGEGYC
                     VFNDSAIASRAVQAAGLVERVVVIDTDVHQGNGTAEILRGDETVFTFSIHGAKNFPFH
                     KEESDLDASLPDGADDTEFLTTLEMGLEAALEAADADLAIYLAGADPFEGDRLGRLSV
                     TKAGLAERDRMVLGACRDRGIPVAVTMAGGYSRNVEDTVDVHFQSIRRAADLLERSVG
                     TDGRTRRS"
BASE COUNT          513 a          980 c          938 g          549 t
ORIGIN      
        1 tgctcgatca atatacagcg cctacattac aacaacgcta accgcagata acattttggc
       61 aacagggcaa gtcttcggta atttctacgt tatcttgagc ttatcgctga ttgctgacag
      121 ctgatagctt tactcacgga tccctgtttt cggcgctttc caggccgtct agcacagggc
      181 cgagttcgcg gtcccgtgcc acttcgtagg cttccaggga ttcggcttcg cgggcgagcg
      241 tcgcgattct tccggtcagg ccgctgccac ctctggcggc gaggatacgc cagccgagca
      301 gccggtaggt gtgttcgagc gtaggattcg cggggctctt ctcaagaaga tcctcgagca
      361 cggcatgcag agcgtccgct tcttttagag ccagggtcag ctctacaatg cggtccccat
      421 gcaagctagc gcgtcttctc gatgtagatg cggtcctggg tgataaccac ctgcagctcg
      481 tccggcgatc cgcccaggta ggatcgccac tcccgctcga agacctcgga gtccctggca
      541 tcagtcttca ggagaacacc gtcttcggtc tcgtcgagga tcgcagagtg ctccgagagc
      601 tggccacgac ggacgggatc tcgacggtaa atggaattcc tggaacggaa gctctgtacg
      661 ctcgaaggcg tcgtgttcag ctcctgcgcg atccaggtgt cacctcgacc ctcttcgacc
      721 cagtgccgga tcctggacgc gtgcggcttg agtgccaccc ttctgtttcc caaggccccg
      781 ttctccttcg accagcctag ttttctaggt tatatgtacc actttttgtt acccgacgct
      841 agatgcatct ctggcctttg tggacagtgc tagcccatcg cctgatacag gatgcagagc
      901 accacgagcc ccctgaggct ccagagcagg gtacgcagcc agttcgacgt tacgagaaac
      961 cggtggaccg cggggtcgaa ccccccgccc agggtagtgt gacgtggtgc ctggagtacg
     1021 gccgtggaga gccagatccc ggcaaccaga attaggccag cccacagcag ggacaccgag
     1081 acacccgccg ggctccagac gaccagcgcc acggcggtcg tcgcctcgag aagcatgggc
     1141 ggcccgacga ccagtccggt cagccgggtg tgcctggcgg agtaggcgga aaagctcgaa
     1201 gggccgacgc gggcgaacag cggatagtgc accacctgta cgaaccagat cacgcctacc
     1261 atgaagagcg tcccggcggc gtgggcgagc aacaggagcg ccatctagcc caggaggtag
     1321 tccgctgcgg ctagccccga gagggccgcg ccctcgacct tgggctggcc gaacccatct
     1381 ccgcagaaga gcagcgttgg atcccctgga gagacgagga acggttctgg gtgggcctcc
     1441 gtcacccagc tgtagcgcca ccgggctaga gaggtctcga cgacaagcga cgagagatca
     1501 gtgccgagcc gatccccggc gaaggagatc aggtcacgcg tgatatccgc ttcctggtcg
     1561 tcgaagtggg cccggctcca ttccggtccc gcgtgaatgg tgatggcggg cgcctcggag
     1621 atgcccttgc ggcggttgtc gccgatccag tccaatggtt cgcctttgat ctgcatgccc
     1681 cccggctctg gcacgttggc ggagccgtca agcagcacca tgagcgccag gcagggatcg
     1741 taagatatgg cctccagatc ctgccgggcg cctgccggta gcccgtagtt ccccgaggtt
     1801 gcgagatcca gggcctgcgg aacgggcatg gcgagcaaca gggctgaagt ttctagccgc
     1861 aggccggact cgcaggagat ctcccagccg gccacacttg cgtcggccct cacggctctc
     1921 tcgccggtgc gcacgtcgag gttgccggcg agatgtttgg ggatggaggt catgccgtca
     1981 gcccccctgt aacgcgggtg gccgtcctcg ttcggcgtgc ccccgtcgtc agtgaaaccc
     2041 agcgaccact ctacggcggc accggcttcg agccaaccct ctacgaggtc agagaaccgg
     2101 tctttcctga ccgtgaagaa ctgggcgccg tggtcgaagg tgcccgctcc cacacgccgg
     2161 gtggccatac ggccaccgac gccacggctt ttgtctagaa cggttacctc ccagccctct
     2221 cgctgcaggg cctcgccggc gagcaggccc gagatgccgg cacccacgat caggcaggtt
     2281 cttgctctct tcgactcgtt caagatcttc gcgtccttcc gtccgttcca acgcttctct
     2341 ctagcaggtc ggcggcgcgt ctgatgctct ggaagtgcac gtccaccgta tcttcgacgt
     2401 ttcgtgagta gccgccggcc atcgttacgg cgaccgggat accgcggtcc cggcaggccc
     2461 ccaggaccat cctgtcccgc tcggccaggc cggccttcgt taccgagagc cggccgaggc
     2521 ggtcgccctc gaacgggtcg gcgccggcca ggtagatggc gaggtcagca tcagcggcct
     2581 cgagcgcggc ctccagcccc atctcgagcg tggtcaggaa ctctgtgtcg tccgcgccgt
     2641 cgggcaggga tgcgtccaga tcgctctctt ccttgtggaa aggaaagttc ttggcgccgt
     2701 ggatcgagaa cgtgaacacg gtctcgtccc cccgcaagat ctccgccgtc ccgtttccct
     2761 ggtgcacgtc ggtgtcgatc acgacgaccc gctctacgag gcccgcggcc tgtacggccc
     2821 tggaggcgat ggccgagtcg ttgaagacgc agtagccctc gcccctgtcg gtgaaggcgt
     2881 ggtgcgtgcc gcccgcgagg ttcgcggcga atccctcctc cagggcggcg aggcaggcgc
     2941 ccacggtccc gcccgaggcg cggcgggagc gctccaccat
//
LOCUS       DBNGVD010000215         4248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_227484, whole genome shotgun sequence.
ACCESSION   DBNGVD010000215 DBNGVD010000000
VERSION     DBNGVD010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_227484"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="AC16CM_06045"
     CDS             <1..1249
                     /locus_tag="AC16CM_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3139426.1"
                     /translation="VVLVVDETPMYRGGENLKEMVANTLRESFELEQVVVGEGEGELH
                     AVEASVAEVEDALGDAGCVVSVGSGTITDICKLATMKGSIPYVAVQSAGSVDGFADDF
                     SVLLKSGAKRTVSSRFPDALIIDLNVLSSAPQEMNAAGFGDILSNFMSSADWYLAHVM
                     GMGGYHRAPPAMLSEQTDDLLDRAAALERREPEALYRLARVLTLSGLAAGVSGSTAPG
                     SGTEHLVGHLLDMSAMQLGRPFAFHGAQVGVACVPVAAAWEALFEGLDPSEVDVDACF
                     PAPDRMEPMVREAFAEIDPSGGVGEECWSEYAKKLVRWSECRPQLESFLSNWDAYREE
                     FQGIVATPDRLGKALEASGAPARFRDLNPPVPQDTAFWALRNCHMMRNRFTLADLLFF
                     LGWWDDAFVERLLERSRTVGGGL"
     gene            complement(1273..2028)
                     /locus_tag="AC16CM_06050"
     CDS             complement(1273..2028)
                     /locus_tag="AC16CM_06050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139427.1"
                     /translation="MSFSGEVAWVTGSSKGIGRATCLGFAERGCDVVVHYSGSREEAE
                     KVAGEVEKHGRDALLIQGDVSDSGEVERMVGEIEDHYGRLDILVNNAGSFINRLTFTE
                     MTEDVWDRVMEVNLKSVYLCSQAILPMMKRHGGGRIVNMTSISARNGGSPNSVAYSTS
                     KGGVSTLTRAMAKELISDGIRVNAVAPGRIDTPLHDEFTPDEKREEAAKRIPEGREGT
                     PEEVVGAVLFLTSSEADYIVGEIIEVNGGLLMD"
     gene            complement(2229..3239)
                     /locus_tag="AC16CM_06055"
     CDS             complement(2229..3239)
                     /locus_tag="AC16CM_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIR3139428.1"
                     /translation="MDRRGSEVARAAWLYYLEELTQGEIARRLGVSRSTVVRLLQRAK
                     ETGMVRVTLNVPHDVFEMERELERLYGLRQVRVVPEAADESEQRRWLGHAAAELLPDL
                     VEPGTVAAVSWGTTLQALAASLRGERAVEGVEVVPLVGGFHRASSGTNAHWVVEQLGG
                     YFRAHTRALYAPVYVEDGSAAEALMRNADIRDTLDLVRRASLAVFSVGSLEPEATMIH
                     LGYLSPDEQAFLQERGAVGDITCRWIDIEGNPVDLPSTINPIGVSLEDLREIPERLTI
                     SGGKRKREALLGTLRGGYATTLVTDDGTASYLLEHVTDPSNSVERELQDIVLDDPPAE
                     TT"
     gene            3675..>4248
                     /locus_tag="AC16CM_06060"
     CDS             3675..>4248
                     /locus_tag="AC16CM_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3139429.1"
                     /translation="MAVGLTESGLRRSRDLESLGQPFDLVVVGGGVNGCGIAWETALH
                     GLRVLLVERDDIGSGTSSWSSRLIHGGLKYLEKLDVRLVRESLREREWLLSSAPHLVR
                     PLRMILPFYSGGKHGPLSLEAGMVAYDVLSWDKSVPHHSVWGKRGVLRRVPGLETEGL
                     SGAAASSVRVTERLEPDADAHERLDEKYGHY"
BASE COUNT          719 a         1373 c         1351 g          805 t
ORIGIN      
        1 ggtggtgctg gtcgtggacg agacgccgat gtaccgtggc ggagagaacc tcaaggagat
       61 ggtcgccaac accctgaggg agagcttcga gctggagcag gtggtggtag gcgagggcga
      121 aggggaactg cacgccgtcg aggcgtccgt ggcagaggtc gaagatgccc tcggggacgc
      181 cggctgcgtg gtatcggtcg gctccggcac gattaccgac atctgcaagc tggctaccat
      241 gaaaggatcc ataccgtacg tggctgttca atcggccggc tcggtggacg ggttcgcaga
      301 cgacttctcc gtgctgttga agagcggggc caagcgaacc gtgtcctcgc gcttcccaga
      361 cgccctgatc atcgacctca acgtgctgtc ttcggcgccc caggagatga acgcggcggg
      421 cttcggcgac attctctcca acttcatgtc ctccgccgac tggtacctcg cacacgtgat
      481 ggggatgggg ggctaccatc gggccccacc cgccatgctc tccgaacaga cggacgatct
      541 gctggatagg gcggcggcgc tcgaacgacg cgagcccgag gcgctctaca ggctggccag
      601 ggttcttacc ctcagcggcc tcgccgcggg cgtatccgga agcaccgcgc cgggctctgg
      661 caccgagcac ctggtgggcc accttctcga tatgtcagcc atgcaactcg gccgcccgtt
      721 cgccttccac ggcgcccagg taggggtcgc gtgcgtgcca gtggccgccg cgtgggaggc
      781 cctgttcgag ggcctggacc cgtccgaggt cgacgtagac gcctgttttc cggcgccaga
      841 tcggatggaa ccgatggtgc gcgaggcgtt cgccgagata gacccgtcgg gcggggtcgg
      901 cgaggaatgc tggagcgagt acgccaagaa gctggtgcgc tggagcgagt gccgcccgca
      961 actggagagc ttcctctcca actgggatgc gtacagggaa gagtttcagg ggatcgtcgc
     1021 cacccctgac aggctcggga aagcactcga agcttctggg gcccccgcgc gtttccggga
     1081 tctgaacccc ccggtacccc aggacacggc gttctgggcg ctcagaaact gccacatgat
     1141 gcgcaaccgc ttcaccctcg cggacctgct tttcttcctg gggtggtggg atgacgcctt
     1201 cgtggaaagg ctcctggaac gctccaggac tgtgggaggg ggcctctaga agcgccaaac
     1261 cggctggggc cctcagtcca tcaacaggcc gccgtttacc tcgatgatct cgccgactat
     1321 gtagtcggcc tcggaagagg tcaggaaaag cacggcaccg actacctctt cgggcgtacc
     1381 ttcccttccc tcgggaattc tctttgcggc ttcttcccgc ttctcatcag gggtgaactc
     1441 gtcatgcaag ggcgtgtcta tgcgccccgg cgcgaccgcg ttcacgcgga tgccgtcgga
     1501 gatcagctcc tttgccatcg ccctggtgag ggtgctgacg ccgcctttgg atgtggagta
     1561 ggcgaccgag ttcgggcttc ccccgtttct ggccgagatg gaggtcatgt tgacgatcct
     1621 gcccccgcca tgccgcttca tcatgggcaa gattgcctga gaacacaggt agacagactt
     1681 gaggttcacc tccatgacgc ggtcccagac gtcctcggtc atctccgtga acgtcagcct
     1741 gttgatgaaa gatcccgcgt tgtttaccag gatgtcaagc cgaccgtagt ggtcttcgat
     1801 ctccccgacc atcctctcta cctcacccga gtcggagacg tcgccctgta tcagcaacgc
     1861 atccctgccg tgcttctcga cctcccccgc aaccttctcg gcttcctccc tgcttccact
     1921 gtaatggacc accacgtcgc agcctcgctc ggcgaagccc aggcaggtcg cccgtccgat
     1981 ccccttgctg ctaccggtaa cccaggccac ttctcccgaa aacgacaagt gctcaacccc
     2041 ctggtcaaat gtttctcata tcgacgcgag ggcaactcta gctcatgttc gcgaccagaa
     2101 caagtggcgg gtctgggcct ggagcgcgac gccgtcgtcc gctgcggttc ttgcggcgag
     2161 ctagggtggg ttgttagcag ggggctaccg ttgccagggc tgatcggcgc cgctggccgt
     2221 cgggcccgtc aggtggtttc ggcgggtgga tcgtccagga cgatgtcctg gagttcacgc
     2281 tccacagagt tgctcgggtc cgtgacgtgt tccaggaggt aagaggccgt gccatcatct
     2341 gtcacgagcg tggtcgcgta gccgccgcgg agggtgccga gcagagcttc acgtttgcgc
     2401 ttccccccgg agatcgtcaa ccgctccggg atctccctga ggtcctccag cgagacgccg
     2461 atggggttga tggtggaggg caggtccacg gggttgccct cgatatctat ccagcggcac
     2521 gtgatgtctc ccacggcacc gcgttcctgc aagaaagcct gctcatcagg cgacaggtaa
     2581 ccgaggtgga tcatggtcgc ttccggctcc aggcttccca cgctgaagac cgccagcgaa
     2641 gcccgcctga cgagatccag ggtatcccgg atgtccgcgt tccgcatcag ggcctcggca
     2701 gcggagccgt cctccacgta gacgggggcg tacagggccc gcgtgtgggc ccggaagtat
     2761 ccaccgagtt gctcgaccac ccagtgcgcg ttggttccgc tggaggcccg gtgaaaccca
     2821 ccgacgaggg gcactacctc gaccccctcc accgctcgct ctcccctgag cgaggccgcc
     2881 agggcctgca gggtggtccc ccaactcacc gccgcgacgg ttccaggctc caccagatcg
     2941 gggagcaact cggccgccgc gtgccccagc cagcgcctct gttctgactc gtctgccgct
     3001 tctggaacga ccctaacctg tctcagcccg tagagccgct ccagctcccg ctccatctcg
     3061 aagacgtcgt gcggcacgtt gagcgttacg cgtaccatcc cggtctcttt cgccctctgt
     3121 aatagccgga ctaccgtgct ccggcttaca cccagccgcc gggcgatctc accctgcgtg
     3181 agctcttcca ggtagtagag ccaggccgcc cgcgcaacct cactgcctct tctgtccata
     3241 atttactcct acgtatctcg ggcgccgccg acctctcctg cgccttccat acggttgtct
     3301 acgacccgaa taccaccaga cagccaaaac gcccgggata gctcccagtt tactgtttcg
     3361 tagtgcgttg tcgagatgga gacacccggt caggcgacat gccccggaag gccgcgcccg
     3421 ggcaccgggg ctattgcacc cgttttcaga aagtgatata aatattctgg tgttgaacaa
     3481 ttgtgctcgt ccctggggaa ggaacttgcg aagcttgtac tacttcctgt ggatgataga
     3541 agaagcccgg gtcaagatat atccacgggc gacagcgtgg cgtgcgggcc cgccgccgga
     3601 tatatgtcgc gggcgcggtg ggagggcgta tacgctggga gcgggatgac gtcttcgggg
     3661 agaggcggta gaagatggct gtcgggctga cagagtcggg tttgcgccgc tcgcgtgacc
     3721 tcgaatcgct gggccaaccc ttcgaccttg tggtggtcgg agggggggtc aacgggtgtg
     3781 gcatagcctg ggagacggcg ctgcacggcc tgcgcgtgtt gctggtcgaa cgcgacgaca
     3841 tcgggagcgg aacatcctcc tggtcgagcc ggctcataca cggcggcctg aagtacctgg
     3901 agaagctcga cgtcaggctc gtcagggagt ctctgcgaga acgggagtgg ttgctctcat
     3961 cggcacccca cctggtccga cctctaagga tgatcctgcc attctactcg gggggaaagc
     4021 acggtcctct ctcgctagag gcggggatgg ttgcctacga cgtgctctcc tgggacaagt
     4081 ccgtacccca ccactcggtc tggggaaagc gcggggtgct ccggcgcgtc cccggcctcg
     4141 agaccgaagg cctgtcgggg gccgccgcgt cctccgtgcg cgtaactgaa cggctcgagc
     4201 ctgacgccga tgcccacgaa cgcctcgacg agaagtatgg gcattacc
//
LOCUS       DBNGVD010000216         2881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4781684, whole genome shotgun sequence.
ACCESSION   DBNGVD010000216 DBNGVD010000000
VERSION     DBNGVD010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4781684"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..820)
                     /locus_tag="AC16CM_06065"
     CDS             complement(245..820)
                     /locus_tag="AC16CM_06065"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HIR3139430.1"
                     /translation="MRTYRIRRRGGALGFLLALVLLMSGLVLVGYSFMIGDPFASAAL
                     AKDPPQNARMTLTVPEMRRVDQVPVYNGVASDEAALHDGTLHVRGTGFPWQEGSNVYI
                     AGHRLGFPGTKSSLVFWDLNSLENGDKVILTDANGTKYTYSVYKTFVVDPNASYVTDP
                     VPGKSVVTLQTCTLPDYEQRLIVRAQLESVG"
     gene            complement(944..1627)
                     /locus_tag="AC16CM_06070"
     CDS             complement(944..1627)
                     /locus_tag="AC16CM_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR3139431.1"
                     /translation="MMIKVQAGKARKMLVSLLGIVLVAAGLGLLVYSVLAGGSPASAE
                     DPGRVQGRISEELEARFNRSIPERIVARVKGDDRPELETPESKRLELTVPGMERVDGV
                     PVYDAPRSGYDRALHDGVLHVRGTGFPWQRQANVYIAGHRVGYPGTESNLVFWDLNKL
                     RKGDEILLTDADGTRYTYRVFEKRTISPSEVGILRPVQGKNIVSLQTCTLPDYSHRLV
                     VRGELVERQ"
     gene            1771..>2881
                     /locus_tag="AC16CM_06075"
     CDS             1771..>2881
                     /locus_tag="AC16CM_06075"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HIR3139432.1"
                     /translation="MKVGIVREVAEGERRVGLVPETVGRLVKDGFEVVVETGAGRDYN
                     LDENYEEAGASVAGGAEEVYGEADIVIKVQSPTEEEIGHLREGQVLVCFLNGPQEPEL
                     VEQLAETGVTLFSNEAVPRTSAAQAMDALSSMGSIAGYKCALIAADTIGKYVPMLSTA
                     AGTTKAANVLVFGVAVAGLQAIAIMNRLGGEVFAYDIRPETKEQAQSLGATFIDSDDE
                     KEDEEEDPYEEYEPTGWRKLMTSLGFYSFAEPPRDKYIVEGEEEEESTEEEAEEGWSQ
                     EKLEEDQKLLRKRMGEMDIVITTALVPGRKAPQLVDKDMVESMKPGSVIVDLAAESGG
                     NCELTEAGETVEHEGVRIVGPVNLPSTMPIHASQLY"
BASE COUNT          550 a          900 c          943 g          488 t
ORIGIN      
        1 acacggtccg aacctcatcg tctatcccgg cggcacctgg tacctgggcg ccacggagca
       61 cgacatcccc gagatagttc gagaacacct ggtcaacggc gaacccgtcg aacacctggc
      121 cgccgaactc aggccccgca aacgcgcaaa accgtagggg aagaacctgc ccccacacgt
      181 tcacgtagtt cggcaccgaa accccttgta ggggcggttc gcgaaccgcc caacggcgat
      241 ggtcctaacc gacgctctcc aattgcgcgc gaacgatgag gcgttgttcg taatctggga
      301 gggtacacgt ctgcagggtg acgacgctct ttccggggac ggggtcggtt acgtaggaag
      361 cgttggggtc gacgacgaag gttttgtaga cgctataggt gtacttcgtg ccgttggcgt
      421 cggtcaggat gaccttgtcg ccgttctcca gcgagttcag atcccagaac acgagggagc
      481 tcttcgtgcc gggaaagccc aggcggtgtc cggcgatgta gacgttggac ccctcctgcc
      541 aggggaaacc cgtacccctg acgtgaagcg tcccgtcgtg aagcgcggcc tcgtcgctgg
      601 cgacgccgtt gtagacggga acctggtcga cgcgccgcat ctcggggacg gtcagcgtca
      661 tcctcgcgtt ttgcggggga tctttagcaa gggccgcgct ggcgaagggg tcgcctatca
      721 tgaaagaata acccaccagc accagcccgg acatcaacaa cacgagcgcc agcaggaagc
      781 caagcgcccc acctcgtctt ctgatcctgt aagtcctcat gcctgtctcc tctccgcaag
      841 cccgggctat ccgcgcctcg gcacgtagcc cgtgattgta gttgcgctac aagatgggct
      901 cccgccgggg acgatgtgtc ccgcgggagc cttgccgtcc ttcctactgc ctctccacca
      961 gctcgccccg cactacgagg cggtgcgagt agtcggggag ggtgcaggtc tgcaggctta
     1021 ctatgttctt gccctgcacg gggcgcagga tgccaacctc gctgggggag atggtgcgct
     1081 tctcgaagac cctgtaggtg tagcgggtcc cgtcggcgtc ggtcaacagg atctcatcac
     1141 ccttccggag cttgttgaga tcccagaata ccaggttgct ctccgtccca gggtagccta
     1201 ccctgtggcc cgcgatgtag acgtttgcct gccgctgcca ggggaaaccc gtgcccctga
     1261 cgtggagcac gccgtcgtgg agtgccctgt cgtagccgct cctcggggcg tcgtagacgg
     1321 gcacgccgtc cacccgctcc atgccgggaa cggtgagctc gagcctcttg ctctccggtg
     1381 tctcgagttc gggacggtcg tcgcccttta ccctggcgac tatcctctcg gggatgctcc
     1441 tgttgaacct ggcctccagc tcctcggaga tcctgccttg cacccgcccg gggtcctcgg
     1501 ccgaggccgg cgagcccccg gcgaggaccg aatagaccag gaggccgaga ccggcggcga
     1561 ccagcacgat tccaagaagg gaaaccagca tcttccttgc cttccccgcc tgcaccttga
     1621 tcatcatcgc cctcctctct cccgttatat ctagggctga taactgaact ttatgtggcg
     1681 caggtgacag aacggttaca aacaggggtt ttgtcggtaa cggttggcca tttcgagcgc
     1741 cggcgcgcct tcgacgctat aatccggcgc gtgaaggtag ggatcgtgcg ggaggtagcg
     1801 gaaggcgagc gccgcgtcgg gctggtgccc gagaccgtcg gtaggcttgt caaggatgga
     1861 ttcgaggtcg tcgtagagac tggcgccggc cgtgactaca acctggacga gaactacgag
     1921 gaggcggggg cgagcgtcgc cggtggcgcc gaagaggtgt acggcgaggc agacatcgtg
     1981 atcaaggtgc agagcccgac ggaggaggag atcgggcacc tgcgcgaggg tcaggtactc
     2041 gtctgctttc tgaacgggcc gcaggagccg gagctggtgg agcagctggc cgaaaccggg
     2101 gtaacgctct tctcaaacga ggcggtcccg cgcaccagcg ccgcgcaggc catggacgcc
     2161 ctctcctcga tgggctccat cgccggctac aagtgcgcgc tcatcgccgc cgacaccatc
     2221 ggcaagtacg tgccgatgct ctccaccgcc gccggcacca ccaaggcggc caacgtgctg
     2281 gtgttcgggg tggccgtggc tggcttgcag gcaatagcca tcatgaacag gctcggggga
     2341 gaggtcttcg cctacgacat ccgccccgag accaaggaac aggcccaatc acttggcgcc
     2401 accttcatag actccgacga cgagaaggaa gacgaggaag aggatcccta cgaggagtac
     2461 gaacccacgg gctggcgcaa gctgatgacc tcgctgggct tctactcgtt cgccgagccc
     2521 ccccgggaca agtacatcgt cgaaggcgaa gaggaagaag agagcacgga ggaagaagcg
     2581 gaggaggggt ggtcgcagga gaagctcgag gaggaccaga agctcctcag gaagcgcatg
     2641 ggcgagatgg acatagtaat caccacggcg ctcgttccgg gccggaaggc cccgcagctc
     2701 gtggacaagg acatggtcga gagcatgaag ccagggtccg tcatagtgga cctcgccgcg
     2761 gagagcgggg gcaactgcga gctcaccgag gccggggaga ccgtggagca cgagggggtc
     2821 aggatcgtgg gccccgtaaa cctgccgagc accatgccca tacacgctag ccagctctac
     2881 t
//
LOCUS       DBNGVD010000217         3381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3506951, whole genome shotgun sequence.
ACCESSION   DBNGVD010000217 DBNGVD010000000
VERSION     DBNGVD010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3506951"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16CM_06080"
     CDS             complement(<1..199)
                     /locus_tag="AC16CM_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139433.1"
                     /translation="MLLRLVGAAVVLLVAGATGVGCGFGGGGGLTLGYLDWNENVASA
                     TLTKVLLEDDLGYDRVELKLAG"
     gene            554..626
                     /locus_tag="AC16CM_06085"
     tRNA            554..626
                     /locus_tag="AC16CM_06085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Arg,seq:cct)
     gene            complement(741..1004)
                     /locus_tag="AC16CM_06090"
     CDS             complement(741..1004)
                     /locus_tag="AC16CM_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3139434.1"
                     /translation="MFSRSNLSRRHFKPILKEAGLDSETRPYDLRHTFATLWMESGEE
                     GILLQRVLGHSRYETTANRYVHPSDRATGEAMSRFGARFAKPS"
     gene            complement(1428..1736)
                     /locus_tag="AC16CM_06095"
     CDS             complement(1428..1736)
                     /locus_tag="AC16CM_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139435.1"
                     /translation="MSEGSGRAETERRIVQRSIEDEAFRQRLVENPKGAVEEELGSQL
                     PEEVRVVTVEESADTVYLVLPGTPMAGAEGEALSDQELEAVAGGWDTGGYDTQADGCL
                     "
     gene            complement(1936..2775)
                     /locus_tag="AC16CM_06100"
     CDS             complement(1936..2775)
                     /locus_tag="AC16CM_06100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3139436.1"
                     /translation="MTENGTPHPDAPGTIVLVHGFWVTPLSWEKWVERYEDRGYRVLT
                     PTYPGFEGGVEALREDPSPIEALTFPAIVEHIGGIIDELDEPPIIMGHSAGGVLTQIL
                     LDHGYGASGVAIDSVPAEGVRVVPVSQIRSLFPILKNPANRHKAVGFTKEQWHYAFTN
                     NLSREESDEVYERYHIPASGRLVWVGATANFTPGHQEAYVDFRNEDRAPLLFIGGSED
                     NIMPPAVNQSNAKHYRYAKSPTHYKEFEGRSHYSVIGGEGWEEVADYALEWAVEHAKR
                     PAA"
     gene            complement(3020..>3381)
                     /locus_tag="AC16CM_06105"
     CDS             complement(3020..>3381)
                     /locus_tag="AC16CM_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3139437.1"
                     /translation="SGNDELIGGSGNDKAQGGPGADELYGTSGTDTLIGGDGNDDLYG
                     GVGDDGELSGGGGTDDISGGWGDDVIRSRDGLPNDFVSCGPNTDTAIVDPDPNDPRSP
                     GDTVSADCEEVRLRLNR"
BASE COUNT          566 a         1128 c         1007 g          680 t
ORIGIN      
        1 ctccggcaag cttgagctcg accctgtcat agcccaggtc gtcctcgagt agcaccttgg
       61 tgagcgtggc gctggccacg ttctcgttcc agtccagata ccccagggta aggcccccac
      121 caccaccgaa tccgcaaccc acgcccgtgg ccccggccac gagcaagacc acagcggccc
      181 ccaccaatct caagagcaac cccgccccac gacggtggct ccttcccaag ctatggtcag
      241 cctccgcagc cgctccttcg gtagcctgca acgtcggatc ccgcgccccg cgtctcacac
      301 ctctcccgtg gcaaaaccag gaatgcgtgc gatccatgcg ccgtacccac ccttcccagg
      361 gcgtgggcac ggatgcatca tacgtcgtcg gcgccgcctg ttcaacttgc cgccgcggac
      421 cctatgaccg cttgcaggat agcgccttgc agcaccccgt cctgctcgcg tcacgctggc
      481 attcgcgtgg gcgaggctag tggacggacg tggtggtaag catggccgcc tcgggttaaa
      541 ctgttggcgc cttgcgcctg tagctcaggg gatagagcag cggtttccta aaccgcgagt
      601 cggaggttcg aatccttcca ggcgcgcttc caaatcggta tatctaaact gattctgtcc
      661 ataacaggac caccatccac aagatcccac cagtcttttc accagcacgg atgcagagaa
      721 ttttacctcg gtgggtccgt tcaggagggt ttggcgaagc gggcaccgaa gcggctcatg
      781 gcctcccccg tggccctgtc ggagggatgg acgtagcggt tggccgtggt ctcatagcgg
      841 ctgtgtccga ggacgcgctg gaggagaatt ccctcctcac cggattccat ccaaagggtg
      901 gcgaaggtat gtctcaaatc gtaagggcgg gtctcggagt cgaggcccgc ttccttgaga
      961 atcggcttga agtgtcgcct cgagaggttc gagcgagaga acagcctcct acgaagctac
     1021 cggactctga gtagtccacc gcacaccgga aactcgcaca ccaggtgcct cagcttcccg
     1081 gcagcggcga gtgatagctg gccgcttttc agcgttgctc cgttcgcagg ctcacggtta
     1141 gggtctctga gcgacccttt aggaacgcac ctctggatac acaaaagcta ctctgggtcg
     1201 ctctgtggcc cgcacagcag agtaacggtc aggacagcga agcgacgcat cgaccttctg
     1261 cgccagcctc tatgagggcc aggagccatc caggacgtac tgcggcccga gccacggagc
     1321 gtcgccgttc gagtcccctt cgctcagcgt tggttggatt gcagcccttt gggtcgagca
     1381 ggggcggagt ctgcctgctc ctcccctcaa ctatcgactg gtcaccctca caggcatcca
     1441 tcggcctgtg tgtcataccc cccagtatcc cagcctccgg ccaccgcctc tagctcttga
     1501 tccgaaagtg cttcgccctc ggcgcctgcc atcggggtgc cgggaagcac caggtatacg
     1561 gtgtccgcac tttcctccac ggtcactacc cgcacctctt cgggaagctg cgagcccagc
     1621 tcctcctcta cggccccctt cgggttctct acgagccttt gcctgaaggc ctcgtcctcg
     1681 atgcttctct gtactatcct gcgctccgtc tcggccctac ctgacccttc gctcacgcgc
     1741 tcccctccct tttcgctctc accccatcgt gcccgggtgt ttgccgggtc tgggttgtac
     1801 gacgccgaca tgttagcaag cgcgaggtag taggtgcctc tgccaggtgg ctacttcttg
     1861 gaacgccgta catgcgaagt tcaacttctg agaaaaccct tccgcgcatt tcggtgaata
     1921 gcgtttgtgg tgcgctcaag ctgccgggcg cttcgcgtgc tcgacggccc actccagcgc
     1981 gtagtctgcg acctcctccc agccttcgcc cccgatcacg ctgtagtgcg aacgcccctc
     2041 gaactccttg tagtgggtgg ggctcttggc gtagcggtag tgcttggcgt tggactggtt
     2101 gaccgccggc ggcatgatgt tgtcctcgct accccctatg aacagaagcg gcgccctatc
     2161 ctcgttgcgg aagtccacgt aggcttcctg gtggcccggc gtgaagttgg cggtagcccc
     2221 cacccacacc agcctgcctg aggcggggat atggtagcgc tcgtagacct cgtctgactc
     2281 ttcacgtgag aggttgttgg tgaatgcgta gtgccactgc tctttggtga agcccacggc
     2341 cttgtggcgg ttggccgggt tcttcaagat ggggaagagc gaacggatct gcgagaccgg
     2401 caccaccctc acgccctcgg ccggaaccga gtctatggcc acgcccgagg caccgtaacc
     2461 gtgatcgagt aggatctggg tgagcacccc gcccgccgag tggcccatga tgatcggcgg
     2521 ctcgtccaac tcgtcgatga tgccccctat gtgctcgacg atggcgggga aggtcagcgc
     2581 ctctatgggg gaggggtcct ccctcagcgc ctcgactccg ccctcgaagc ctgggtaggt
     2641 cggggtcagc acgcggtagc ccctgtcctc gtagcgctcc acccacttct cccaactgag
     2701 cggtgtcacc cagaagccgt ggaccaagac gatggtgccg ggggcgtcgg ggtgcggggt
     2761 gccgttttcg gtcaaggggg cctcctgagt gagccgtttc cttccggtac caagtgtgcc
     2821 tcatgcgaag tttcggcgca tcctcccgtc ggccgaactt ccagaagcgc gcgtaggcga
     2881 agttccgaga acgccgtaca tgcgaagttc gcagaatgat ccttccacgc actagcatga
     2941 ataagggcga gaggtggggc taggctaatg tgccaggacc cggatccgtc gccgacggca
     3001 agagctcttg tgcccgcttc taacggttga gccggagcct cacctcttca cagtcggcgg
     3061 ataccgtgtc gcctggcgac ctgggatcgt tcgggtcagg gtccacgatg gcggtatctg
     3121 tgttgggacc acagctgacg aagtcgttag gcaggccgtc gcggctgcgg atcacgtcat
     3181 cgccccagcc ccctgagatg tcgtccgtgc caccaccacc agagagctcg ccatcgtcgc
     3241 ccactccccc gtagaggtcg tcgttgccgt ctccgccgat cagggtgtcg gtgccggagg
     3301 tgccgtagag ctcgtcggcg cccggaccgc cctgggcctt gtcgttgccg gatccaccta
     3361 tgagctcgtc gttgcccgaa a
//
LOCUS       DBNGVD010000218         7209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4054114, whole genome shotgun sequence.
ACCESSION   DBNGVD010000218 DBNGVD010000000
VERSION     DBNGVD010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4054114"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CM_06110"
     CDS             complement(<1..815)
                     /locus_tag="AC16CM_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139438.1"
                     /translation="MFWRGVGETPDEGVAETPEEHTGPIHIERGAPRPATILRVAGEL
                     EARGGTIVELFKEIDSPLGRVVVPIHFRQDDQDFLVEIATEPWNGRRSREAVDRAAIV
                     RNSEYAEAGLEILSAYPVPSEVGFFFGRSPAAMLQLDLARLTPDVPEVAAGVFREVAS
                     SHWGVDLNYEPAYLPLVEELLLAVLDADEEEGAPPLSDGLVAGLGCFLGETIRRNAHP
                     PGAWRPPEEWGAGPVVELDGFVLDPVGKARAFLENGPQESMAFYVQFTLELLE"
     gene            906..1559
                     /locus_tag="AC16CM_06115"
     CDS             906..1559
                     /locus_tag="AC16CM_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3139439.1"
                     /translation="MPNSRELILVRHGQSTANAEGIWQGQLDFPLSDEGRRQAGQAGT
                     ALSRERLDGLYASPLSRAFETASIIRSSSGFRGEVVAVEGLSERGGGILEGYTWAEQE
                     RRNPALAEKFLAIPEEDRWALVGAETDEEVIARFEEAISSIVTRQPTGSRIVVVSHGG
                     VMRAYLRELFGEEVLPDAQRAANASITRMRWDDAGPHLLELAVTAHLAEDPGFTNME"
     gene            complement(1618..1824)
                     /locus_tag="AC16CM_06120"
     CDS             complement(1618..1824)
                     /locus_tag="AC16CM_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3139440.1"
                     /translation="MPREQGRVKWFDAEKGYGFLVRPTGDDLFVHHSEVQGDPSELSP
                     NGEVEYEVGRNDRGPNARRVRSLG"
     gene            complement(2463..3470)
                     /locus_tag="AC16CM_06125"
     CDS             complement(2463..3470)
                     /locus_tag="AC16CM_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139441.1"
                     /translation="MVELTDKIAEAAWRIRNLEDYDGRLERAVRRFDEVTRARPEPAM
                     VVYETALMKSGRSEDEVRALVAEFRETFEDEEEPLTIPHASGIMNVEGDGWFFGDEDL
                     RVLTGRLLGQFQHRVAQYNYVDEPEVMKRWADGYDTRLFVRRRIHGTEPVVGVVGGLD
                     LMLFQYLRVAGGGNTLVPTENVARTLGALGYEGDAYEVLANGETLAIYLELPAPIVGE
                     MLEDLGREGFTDFPEPPRETAEPEETGSVDDGNAVTDDGSAAGDAGSEEGVQKPSPED
                     RAVRRATREDSGSGEEPSRVQDPQAQDAPGVSEEGGGKKQASAPEGGRGDAPGTADGD
                     R"
     gene            complement(3564..3803)
                     /locus_tag="AC16CM_06130"
     CDS             complement(3564..3803)
                     /locus_tag="AC16CM_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3139442.1"
                     /translation="MPEGVDFKAAAREVLGEVGHPLHYSDITEIALHSGYLHSAGRTP
                     QNTMRARLSVDVRDNPDSPFVQRAPGVYGLKAPNP"
     gene            complement(3796..4851)
                     /gene="pheA"
                     /locus_tag="AC16CM_06135"
     CDS             complement(3796..4851)
                     /gene="pheA"
                     /locus_tag="AC16CM_06135"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3139443.1"
                     /translation="MKIDAIDEEIVRLLDRRAWLARRIGQIKQDNGLAAYAPARERVV
                     LDRVAALSEGEFPKRGLQAVFREIISSSISLEGRLKVAYLGPEATFTHEAALRSFGSS
                     IELEPQVTVAEVFARVERGEAQHGVVPVENSMEGAVTHTLDELMNSPLKICGEVYLPV
                     SQNLISSETSMERVSMVCSHPMALAQAASWLRRELPWATLEDVDSTAEAARRAAEEPG
                     VAAVGSVLAAGTHNLKVLARNIQDARTNTTRFIVLGREWAARTGRDKTSVVFSVKDRP
                     GVLKDALSAFSEQGINLTRIESRPSRKRAWTYVFFADLLGHPDEDRVRQAMESLEEHC
                     PYVSLIGAYPEVAPEIA"
     gene            complement(5171..5992)
                     /gene="uppP"
                     /locus_tag="AC16CM_06140"
     CDS             complement(5171..5992)
                     /gene="uppP"
                     /locus_tag="AC16CM_06140"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HIR3139444.1"
                     /translation="MLEFIEAIFLGVVQGLTEFLPVSSSGHLLLGQYFLGLDENRFGL
                     SFDVALHMGTLVAVVTYFWRDLIRMALAFLRSFGHRDLENDPDQRLAYLVIASTVPAA
                     LIGFLFEGFFENVVRSPWVVVFNFVLVGILFLVAEAAGSHRRKASSLGFGAAVGIGIA
                     QATALVPGVSRSGATITLGLFLGLRREEAARFSFLMSVPIIAGAGSLQLGEVLATGMS
                     AHQWLLFLVGVVTSAAVGYVTIRFLLNYLTHHSLRVFAYYRFAVAGVVAVLLMLF"
     gene            complement(6305..7000)
                     /locus_tag="AC16CM_06145"
     CDS             complement(6305..7000)
                     /locus_tag="AC16CM_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3139445.1"
                     /translation="MPVDVSVTEYTKLQEQECRALLKLLGEMGIATADRVYGPGDAIY
                     NEGEYGDALYVLTQGVLKLFRPYSGSKEATLRLLKPWDIFGHLAFAGETRQRAYAEAV
                     TECRVTKVPKVFVERAVRQEPRAAFKLMTLLELRLVQYEELVQCLLPRETEVRLANLL
                     PLLAQKFGESKNGLVTITLRLTHQDLAAMVASTRESVTKVLNEMRSRGVIEIESGRIT
                     LKDTSALTRLSRS"
BASE COUNT         1260 a         2396 c         2262 g         1291 t
ORIGIN      
        1 tcctccagca gttccagcgt gaactgaaca tagaaggcca tcgattcttg cgggccgttc
       61 tcgaggaagg ctcttgcctt tccaacgggg tcgagtacga aaccatccag ctcgacaact
      121 ggcccggccc cccactcctc gggtggtcgc caggcgcctg gcgggtgcgc attgcgtctg
      181 atggtctcgc ccaggaagca ccccaggccg gccacgaggc cgtcggagag tggcggggcg
      241 ccctcctcct cgtcagcatc gagcacggct agcagcagct cttcgaccag gggaaggtag
      301 gcgggctcgt agttcaggtc gacgccccag tggctgctcg ccacctccct gaagaccccg
      361 gcggccacct ccggcacgtc cggcgtaagc ctcgcgaggt ccagttgaag catggcggcc
      421 ggcgacctgc cgaaaaagaa ccctacctcc gagggtacgg ggtaggcgct caggatctcc
      481 aggccggcct cagcgtactc cgagttgcgc actatggcgg cccgatcgac ggcctcgcgg
      541 gatctccgac cgttccacgg ttcggtggcg atctcgacca ggaagtcctg gtcgtcctgg
      601 cggaagtgta tcggcacgac cacccggccg aggggggagt cgatctcttt gaagagctcg
      661 acgatcgtgc ccccgcgagc ctctagctcc ccggccacgc gcagtatcgt ggcgggacgg
      721 ggtgcgcccc tttcgatgtg aatggggccg gtatgctctt ctggggtctc ggctacgcct
      781 tcatccggcg tctcccccac gcctctccag aacatccagc cagtccttcc tcaaaagcgc
      841 gaacgagcaa attctaacat cggtcaggat ttgccggcgg cgctcccatg cactaccctt
      901 atccgatgcc gaactcgcga gaactaatac tggtgcgtca cggtcagtcc acggccaacg
      961 cggaggggat ctggcaaggt cagttagact ttccgctctc agacgaaggc cgaaggcagg
     1021 ccgggcaggc gggtacggcg ctgtcccgcg agcggctcga tggtctctac gcgagccccc
     1081 tctcccgtgc cttcgagacc gcgagcatca tccgctcgag ttcaggcttc cggggggagg
     1141 tcgtcgccgt ggagggcctt agcgagaggg gcgggggaat actcgaaggg tacacctggg
     1201 cggagcagga gagaagaaac cctgcgctgg cggagaagtt tctcgccatc ccggaggagg
     1261 accgttgggc gctcgtcggg gcggagaccg acgaggaggt catcgccagg ttcgaggaag
     1321 ccatctcctc gatcgtcacc cgccaaccca cgggctcacg catcgtcgtc gtgtcgcacg
     1381 gcggcgtgat gcgggcctac ctgcgtgaac tcttcggaga agaagtatta cccgacgcgc
     1441 agcgggctgc caacgcttcg atcacgcgaa tgcggtggga cgatgcggga cctcatctgc
     1501 tggagttggc ggtaacggcg cacctcgcag aagaccccgg cttcacgaac atggaatgat
     1561 ccggtagagg tgtaattatg cgggaagtgg acgcggggtc cgcgaaccgc cccttcgtca
     1621 gccgagggac ctgacgcgtc gggcgttcgg gccacgatcg ttgcgcccga cctcgtactc
     1681 gacctccccg ttgggggaga gttcggaggg gtcgccctgc acctcggagt gatggacgaa
     1741 caggtcgtcc ccagttggac gcaccaggaa accgtatccc ttctcggcat cgaaccactt
     1801 gacgcggccc tgctcgcgcg gcaaatcgtc gccggcgggg ggtgctgtct cctcgggctc
     1861 aatatcctgc gccacggctg ccaccaccgt atcggccgag ccgtctgccg aggcgggctc
     1921 taccccgtcg gcctcgagca cgatggagtc ttcggctacc tttacgacca ctgcctccac
     1981 cctggcccag tgctccctcc agataggatc ctccgcgatg gaaggatcga gccagaggcc
     2041 ccagccgtcg cgctccccgt ggtcgagaac tccctcgaag acggtctctg gttcacgtgg
     2101 gccgcgttcg cggcggtaga tgtcgttgcg cgagcgaaag gactgtacgg aggacgcgct
     2161 cgtgccgaga gcgtcggcga tccagtcgtc cgttttgccc gcctccaccc actccctgat
     2221 gcgatccatg tgtggtttca acgctatgcg ctttctggag tctgccatgc taccgcttgc
     2281 tccttcttcg aacccgttct gataagtgcc gcgctaagcg attgatagac gagctgttgt
     2341 tagtagatgc ctgagctagt ctaccatttc ctccgggcta gagagcggtt ctgccaggaa
     2401 accgtttctc gacacggacc gacgggggcc aggatccggt agcccggctt cgggctccgt
     2461 ccctacctgt ccccgtctgc cgtgccaggg gcatctcccc gcccaccttc gggagcggac
     2521 gcctgcttct tgccgccgcc ctcttcggac acacccgggg cgtcctgggc ctgcgggtcc
     2581 tgtaccctag agggttcctc accgctacct gaatcctcgc gggtggctcg gcgcacggcc
     2641 cggtcctcgg ggctcggctt ctgcacgccc tcttcggacc cagcgtcgcc ggcggcggag
     2701 ccatcatccg ttaccgcgtt gccgtcgtcg accgaccccg tctcttccgg ctcggccgtc
     2761 tcgcgtgggg gctcggggaa atcggtgaag ccctcgcgtc cgagatcctc gagcatctcc
     2821 ccaacgatcg gcgcgggaag ctccaggtag atcgccaggg tctccccgtt agcgagcacc
     2881 tcgtaggcat caccctcgta gcctagggcc ccgagtgttc tggcgacgtt ctccgtcggc
     2941 acgagggtat taccgccgcc cgcaacgcgc aggtactgga acagcatgag atccagcccc
     3001 ccgaccacgc caacgactgg ttcggtcccg tgaatgcggc gcctgacgaa gaggcgcgtg
     3061 tcgtagccat cggcccagcg cttcatcacc tcgggctcgt ccacgtagtt gtactgggca
     3121 accctgtgct ggaactgtcc gagcagacgc cccgtcaaca cccgcaggtc ttcgtcaccg
     3181 aaaaaccacc cgtcgccctc cacgttcatg atgccgctgg cgtgcgggat ggtaaggggc
     3241 tcctcctcgt cctcgaacgt ctcacggaac tcagcaacca gcgcgcgaac ctcatcctcg
     3301 ctgcggccgc tcttcatcaa ggccgtctcg tagacgacca tcgcgggctc ggggcgcgcc
     3361 cgcgtgacct cgtcaaacct gcggacagct ctctccaggc gcccgtcgta gtcttcgagg
     3421 tttctgatcc tccacgccgc ctcggcgatc ttatccgtaa gctcgaccaa cgtctctcct
     3481 cggtgttata gaaagcaatc gcccgcaaat atagcatgcc ggcacgccgg ggctggagtc
     3541 ccggcgtgcc ggggactttc ccatcacggg ttgggggcct tcaggccgta gactccaggt
     3601 gccctctgga caaaggggct atccgggttg tctcgcacgt cgacagagag ccgcgccctc
     3661 atcgtgttct ggggggtccg gccggcgcta tgcaggtagc cggagtggag cgcgatctcg
     3721 gttatgtccg agtagtggag tgggtggccg acctcgccga gtacctcgcg cgctgcggcc
     3781 ttgaagtcca ccccttcagg caatctcggg agcgacctcg gggtaagccc cgatgaggct
     3841 cacgtacggg cagtgctcct cgagtgattc cattgcctgc ctcacccggt cctcgtcagg
     3901 gtgtcccagc aagtcagcga agaacacgta ggtccacgcc cgcttgcggc ttgggcggct
     3961 ctcgatgcgg gtgaggttga tcccctgctc cgagaaggcc gaaagcgcgt ctttcagcac
     4021 gcctggcctg tccttgaccg agaagaccac gctcgtcttg tccctacccg tcctagcggc
     4081 ccactcacga cccagcacga tgaagcgggt ggtgttcgtg cgggcgtcct ggatgttgcg
     4141 cgccagcacc ttcaggttgt gggtacccgc cgccagcacg ctcccgacgg cagcgacccc
     4201 cggctcttct gccgcccgcc gggcggcctc ggccgttgag tccacgtcct ccagggtcgc
     4261 ccagggcagc tcgcgcctga gccagctggc cgcctgggcc aaggccatgg ggtgtgagca
     4321 caccatgcta acccgctcca tcgaggtctc ggaggagatc aggttctgcg agacgggcag
     4381 gtagacctcg ccgcagatct tcagggggct gttcatgagc tcgtccaggg tgtgcgttac
     4441 ggcgccctcc atggagttct cgacgggcac gacaccgtgc tgggcctccc cacgctcgac
     4501 cctcgcgaac acctcggcga ccgtgacctg tggctcgagc tcgatgctcg acccgaacga
     4561 gcgtagagcc gcctcgtggg tgaaggtggc ctcgggaccc aggtaggcga ccttgaggcg
     4621 tccctccaac gagatggagg acgagatgat ctcacggaac acagcctgca gaccgcgctt
     4681 cggaaactcc ccctcgctga gcgccgcgac ccggtccagc acgacccgct ccctggccgg
     4741 ggcgtaggcc gcgaggccgt tatcctgctt tatctgtcct atccgccgcg caagccaggc
     4801 acggcggtcg agcaggcgca cgatctcctc gtcgatcgcg tcgattttca ctcggatctc
     4861 ccccatctcc tcgccggggg aaacgtcggc aacacccgcg tctcgctgcg ggttttctcg
     4921 tttgtgtgcc tggtcaggtt gcgtcatggc gtgtgcggtc tcctgtggaa gtttgacggg
     4981 gtttggatca ggaggagcgc ggccgcgcgt caggggcggg agcgttcctc ccggataaat
     5041 cgccgcaacg cttcgaccgc ctgtagcatg gtgcgatcaa cctaccagac gactgccccc
     5101 cgtgtcaaag ccacaaaccc gcatttcacc aggtcaagaa cggttcgcga accgcccgaa
     5161 tacacgttgc ctagaacaac atcaacagga ccgcgacgac gcccgcgacg gcgaaacgat
     5221 agtaggcgaa gacgcggagg ctatggtgcg tcaggtagtt gagcaggaag cgtatggtga
     5281 cgtacccgac ggcggccgag gtgacgacgc ccacgaggaa cagcaaccac tggtgggcgc
     5341 tcatgccggt cgcgagtacc tcccccagtt gcaggctgcc ggccccggcg atgatcggga
     5401 cgctcatcag aaacgagaac cgggccgcct cctcgcgcct caggcccaga aacaggccga
     5461 gcgtgatggt agccccagac cgtgacacgc caggcacgag cgccgtggcc tgggcgatac
     5521 ctatcccgac cgcggcgcca aacccgagac tggaagcttt ccgcctgtgg ctcccggcgg
     5581 cctcggcgac gaggaacagt atgccgacga ggacgaaatt gaacacaacc acccagggcg
     5641 agcgtaccac gttctcgaaa aagccctcga aaaggaaccc gatcagggca gccggtacgg
     5701 tggaggcgat cacgaggtag gccaaccgct ggtctgggtc attctcgagg tccctgtgac
     5761 cgaatgacct caagaaggcc agcgccatcc tgatcaggtc cctccagaaa tatgtgacca
     5821 ccgccaccag cgtccccata tgcagggcca catcgaaaga caatccgaac cggttctcat
     5881 cgagcccgag aaagtactgc ccgagcaaga ggtgtcccga gctggagacc ggcaggaact
     5941 ccgtgagccc ctgtaccacc cccagaaaga tagcctcgat aaactcaagc acgagagcac
     6001 gctccggctc cgcctacgct tgtcagcacg ccggcttgcg ccggcttgtc agcacgctcg
     6061 acttcgcctc gcttgtcggc aacgcttcgg cttcgcctcg ctttcggcca gtcgtttcgg
     6121 gccgtcacta aatgctcggc aagggaccat cctgcgcgcc ggtgctcacc gtgcgtaagg
     6181 tgcgggccgc agcgggatct ggctgaaaac gagccccagg cgaagccggg cgaagcgtgc
     6241 cgacgagcga gggcgccagc ccgagcgtgc tgacaagcgt aggcagcagg ccgaagcgcg
     6301 ctcttcagga acggctcaat ctggtcaggg cactcgtatc cttgagggtg atacgaccgg
     6361 actcgatctc tatcacgccc ctgctgcgca tctcgttcag gaccttggta acggattctc
     6421 tggtggacgc gaccatagca gcgaggtcct ggtgggtgag gcgcagggtt atggtaacga
     6481 ggccgttctt gctctcgccg aatttctggg cgaggagcgg caggaggttg gcgagccgca
     6541 cctcggtctc gcgggggagg aggcactgga ccagctcttc gtactgtacc aggcgcaact
     6601 caagcagggt catcagcttg aaggcggccc gcggctcctg tcggacggcg cgttcgacga
     6661 acaccttggg aaccttggta accctgcact cggtaacggc ctccgcgtag gcgcgctgcc
     6721 tggtctcgcc cgcgaacgcc aggtgaccaa agatgtccca gggcttgagc aggcgcaagg
     6781 tagcctcttt gctgccggag tacggccgga aaagcttcaa gacaccctgg gtcagcacgt
     6841 acagggcgtc gccgtattca ccctcgttgt agatggcatc cccgggaccg taaaccctgt
     6901 ccgcggtggc tatgcccatc tcgccaagaa gcttaagtag cgcccggcac tcctgttctt
     6961 gcagcttcgt gtactcggtc acggaaacgt caacaggcac gggtgtccct ttcaactagt
     7021 gccggcaggg tttcacgatg gtactcacat ggcttctcgc tgagcgtgct acacattgct
     7081 acattgtaca ttacgcggga ctgcaaacac gagccccgcc gattaggtta gaatgcgcgc
     7141 aggttcgtat tcgagagagg agaccaggtg cgggaacgtc tagcgttcgg gattttgatc
     7201 gtgttgctg
//
LOCUS       DBNGVD010000219         2167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2778188, whole genome shotgun sequence.
ACCESSION   DBNGVD010000219 DBNGVD010000000
VERSION     DBNGVD010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2778188"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="AC16CM_06150"
     CDS             <1..1792
                     /locus_tag="AC16CM_06150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3139446.1"
                     /translation="VVVLLSWLLFDPVRMDEVSLGVVASGLYVMNWLLAARATDYFAA
                     GLQASPVQHFWTLAVEEQFYLLWPALLLLGAWWSRRTGRGLRPALALAFAAIVVSSLA
                     YSVYSTESQAGAAYFSTLTRAWELALGGLIALIPVSRLTNLPRVWAGLAAWAGLGAIA
                     FSTVRFNDDTLFPGYAALIPTLGTAAVIAAGFATVSGENPTGAARLLTLGPVRHVGRL
                     SYSWYLWHWPPLVFAATVWGSLSPLQGMAVLAVSYVPALLTNRLVEKPFLHSETLTRF
                     PRKALALGGVCTSLSVALGVLLFALTPSIPEAPESQVAGAAALRNGHALQTSARAVHP
                     TPREAETKENRPRMYADGCHLDLTEVEAPRCVYGDPSSDTTVVLFGDSHAMQWFPALN
                     RLADERGWRLVGITKSACPPAQVSIYNAGLRREYTECDRWRELTLDRIVQQEKPDLVV
                     TSSLPTYKPRENGKRLRGQASEEAMVEGYASTLEDLRSTGASVALLEDVPHPDKNVPE
                     CVSRSLDNLERCAFPRSKALNVPPVNPKAAEKVEGVRLIDPTPVVCPEKSCPSVIGDA
                     LVYRNGAHLTATYVRSMTPWLGKQLPEPAG"
BASE COUNT          335 a          739 c          715 g          378 t
ORIGIN      
        1 ggtcgtggtc ttgctgtcgt ggctactgtt cgatcccgta cgcatggacg aggtatccct
       61 gggggtcgtc gcgtccggcc tgtacgtgat gaactggctg ctggcggcgc gtgccacaga
      121 ctacttcgcg gccgggttgc aggcgagccc ggtccagcac ttctggacgc tcgcggtgga
      181 ggagcaattc tacctgctgt ggcccgcgct cctcctgctc ggcgcctggt ggagcaggcg
      241 tacgggacgc ggcttgcggc ctgcgcttgc cctggccttc gccgccatag tggtctcgtc
      301 gctagcctac agcgtctact ccacagaatc ccaggccggg gctgcctact tctccaccct
      361 gacacgcgcc tgggagctcg cgctcggcgg cctgatcgcg ctgataccag tctcgcgcct
      421 cacgaatctt cccagggtgt gggccggcct tgcggcgtgg gcggggctcg gcgcgatcgc
      481 cttctccacc gtacgcttca acgacgacac cctgttcccg ggctatgcgg ccctgatccc
      541 gacccttggc acggcggcgg tgatcgcggc gggcttcgcg accgtctcgg gagagaaccc
      601 tactggggcg gcacgcctgc tcaccctcgg tccggtaagg cacgtaggac ggctctcata
      661 ttcgtggtac ctctggcact ggccgcccct cgtcttcgcc gccacggtgt ggggaagcct
      721 gtccccgcta caaggcatgg cggtcctggc ggtctcctac gtccccgccc tcctgaccaa
      781 ccgcctcgtc gagaagccct tccttcattc ggagacgctc acccgcttcc cacgcaaggc
      841 cctcgccctc ggtggggtgt gtacctcgct gtcggtcgct cttggtgtgc tgctcttcgc
      901 gttgacgccg agcattccag aggcacccga atcccaggtt gccggggccg ccgccttgcg
      961 gaacggtcac gccctccaga cgagcgcaag ggcggtccat cccacgccgc gagaggcgga
     1021 gaccaaagag aaccgtccgc ggatgtacgc cgacggctgc cacctcgacc tcaccgaggt
     1081 cgaggccccc cgatgcgtct acggcgaccc ctcctccgac accaccgtgg tccttttcgg
     1141 agactcccac gccatgcagt ggttccccgc cctgaacaga ttagccgatg agcggggctg
     1201 gcgcctggtc ggcatcacta agagcgcctg cccccccgcg caggtgagca tctataacgc
     1261 cggcctgcgc agggaataca cggagtgcga ccggtggcgt gagctcaccc tggatcgcat
     1321 cgtccagcaa gagaaacccg acctggtggt gaccagcagc ctgccaacct acaagccgag
     1381 ggagaacggc aagaggctgc gtgggcaagc tagcgaggag gctatggtgg aaggctacgc
     1441 ctcaaccctc gaagatctgc gcagcaccgg cgcctccgtg gccctcctgg aggacgtgcc
     1501 acacccggac aagaacgtgc cggagtgtgt ctcgcgctcg ctggacaacc tggagcgttg
     1561 cgctttccca aggagcaagg ccctcaacgt ccctccggtc aatccgaagg ccgccgagaa
     1621 ggtcgagggg gtgcggctga tcgacccgac acccgtggtg tgcccggaga agagttgtcc
     1681 ctcggtaatc ggggacgccc tggtgtaccg caacggggcc cacctgaccg cgacctacgt
     1741 acgctccatg accccctggc tcggcaagca actgcccgag ccggcgggtt gaccggcggt
     1801 aggtccccag aggtttatcc ggtccaggcc ccctcgaacg cgttattata gtgaaggtcc
     1861 tggcccgccg atcccgtctc gaggcgggat cgcgagcagg tccggccggc tgagagcgct
     1921 ttctcgaacc acctgttagc gggagaccag ttccgggggt gcgtcctcgc atatctcgag
     1981 gactgctcct cggtgttccg gcccgatggg atatgaggga gccaggctct agcgagtagg
     2041 cgggatgaga ggacgtctcg ggaaggatga agccaggagt gaacgcacga tgagggcggg
     2101 ccgacgtgcg gccgtgggca ggtggaccgg cgccgcggtc gttttcgcgt gggctctgct
     2161 cgggctg
//
LOCUS       DBNGVD010000220         5544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1867569, whole genome shotgun sequence.
ACCESSION   DBNGVD010000220 DBNGVD010000000
VERSION     DBNGVD010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1867569"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="AC16CM_06155"
     CDS             complement(<1..1032)
                     /locus_tag="AC16CM_06155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HIR3139447.1"
                     /translation="MIGDAPRILSVATALPPHRIGQEEAKTFARGMFSRRDGDIERLL
                     PIFDNVNVDSRYFCVPVEWFERDHGFPEKNALYVEHALDLSEKAASKALDRIGAKPED
                     VGAIFFVSTTGISTPSLDSKLIFRLGLSEHTRRVPIWGLGCSAGASGLARASEHARLY
                     PDKLALLVAVELSGLTFQRGDRSRSNLISTSLFADGAAAVLLGDSTGPEILGSYSTTW
                     PDTEDVMGWELVETGLKVQLSKSVPAIVQARFRENLEEACASLGLDFDELEHFVLHPG
                     GSKVLDAFENVLGLESGGLATSRGVLRDCGNMSSVTVLFILERFLESREFTRGELGVL
                     SAMGPGFSAE"
     gene            complement(1029..1865)
                     /locus_tag="AC16CM_06160"
     CDS             complement(1029..1865)
                     /locus_tag="AC16CM_06160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3139448.1"
                     /translation="MDFLDVLKRRKTTNGPFLPDPVRPEHQRLLVEVAAMAPSHFNSQ
                     PWRFVLSDDRETIEEVARISGDSMTRLLEEGTFWKRYRPYFRFSEAEMEQRRDGIHFD
                     QMPAVLKPFRRQIFSSTGQAVMNRFGVPKTLGEDNRKLVAGSPLLLAVLLDKTEYRPG
                     ELSGFYSVFGMGAAVENIWLTTTELGMGIQFVSTPMEIPENWERLKRLFKVPEDLELM
                     AVYRLGYVPTEQRRPTIDWSSRQRKRLSQYVFRNTCETPEPDAGAGTEPGVSRQASPG
                     KN"
     gene            complement(1875..2183)
                     /locus_tag="AC16CM_06165"
     CDS             complement(1875..2183)
                     /locus_tag="AC16CM_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139449.1"
                     /translation="MIFYVATVFFVANFVLGILVQFRVVDTKPFRWLHHALFFAAFVS
                     AAAAAGVGFLQGAPYRWVLLPVLMLFTVLPRVRAGTPGHAALASGALILYATGCVWML
                     "
     gene            2353..3594
                     /locus_tag="AC16CM_06170"
     CDS             2353..3594
                     /locus_tag="AC16CM_06170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3139450.1"
                     /translation="MAEKRPEAYITGIGVVTPIGVGREKFWGSLLAGESGAGPITLFD
                     PEGYDVRIAAECSDFDPESFMDRKVAARTDRFGQMGLASAKLALEDADAWDVLNQEPE
                     RVGLILGSGLGGVTSMESTQATIDSKGPGRVNPFAVTKIMPNSAAAHVGIQLGVQGPS
                     IAGALACTCGTDMMALGYDLIRRGDADVVLCGASEAALTPVIVAGFIAMRAMSRRNDD
                     PEGACRPYDVDHAGFLIGEASAVMVLESAESMERRGSEPYAKITGVGRTTDAYNLTDP
                     DPGGHGIRRAMQLAVADAGLEGEQIGYINPHGTGTVAGDAPESQAMAAINPDVKVSAT
                     KSTLGHSLGASGALETAICALAMKERTIPPMRNLTEVAEGCADLDYVVDEPREVPDLQ
                     AAICANLGVGGHNVAISLERA"
     gene            3591..4154
                     /locus_tag="AC16CM_06175"
     CDS             3591..4154
                     /locus_tag="AC16CM_06175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3139451.1"
                     /translation="MSDVYTVPEGLPEPTDDGACDHLPGLDLPTVSLTSTSGDEVDLS
                     ELAGTTVVYCYPLTGRPDRELPGGWDEIPGARGCTPQSCAFRDHHAELEDLGARVFGL
                     STQTPDYQREAAQRLHLPFPLLSDQNLEFAEALDLPTFGAEGMTLVKRVTLIVEDGEI
                     RKVFYPVFPPGRNAEEVILWLSQQATS"
     gene            4168..5022
                     /gene="larE"
                     /locus_tag="AC16CM_06180"
     CDS             4168..5022
                     /gene="larE"
                     /locus_tag="AC16CM_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR3139452.1"
                     /translation="MNPVPTDVLQEKLTELEGTLAPYGSALVAFSGGVDSSLALAVAA
                     RALPRHRVLAVTSNNETYLPSELDLAREFADSLGVEHLVVNTRELDNPNYASNPTNRC
                     YFCKSTLYSDLARLAREKGYACVVDGANKDDEGDYRPGRKAAKELGVISVLSAARMSK
                     EEVRELAKHLGLPTWDKPALACLSSRFPYGQEITPEKLTQVARAEEFLRREGFQQVRV
                     RHHGEIARLEVGPGELERAFAMREKISAELLDAGFLYVTLDLAGYKSGSLNRALKKDK
                     KALPLIST"
     gene            5025..>5544
                     /locus_tag="AC16CM_06185"
     CDS             5025..>5544
                     /locus_tag="AC16CM_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR3139453.1"
                     /translation="MDNVEGPLLALTIVLMAVGLVGCVVPGLPGVTLIFLSALLYAIL
                     TDFETVGTLVLVILFVLAALAFLTDFVATSYGARRFGASNWGTVGGAVGGIVGAVAGL
                     VFAGVGSLFGLILGSIAGVFIGEYLRRQRRGDQERQSTDWRRASRAAGGVIVGYLASA
                     VIQGLLGLASIII"
BASE COUNT          998 a         1772 c         1853 g          921 t
ORIGIN      
        1 ctccgctgag aagcccgggc ccatcgccga gagcacgccg agttcgcccc tcgtgaactc
       61 gcggctttcg aggaaacgct ccaggatgaa gagcacggtg acgctcgaca tgttgccgca
      121 gtcgcgaagc acacccctgc tggtagcgag gcccccagat tcgaggccga gcacgttctc
      181 gaaggcgtcg agaaccttgg agccgccggg gtggagcacg aagtgctcga gctcatcaaa
      241 atcgaggccg agggacgcac acgcctcttc caggttctcg cggaacctgg cctgcacgat
      301 ggcgggaacg ctcttggaga gctggacttt caggcccgtc tcgaccagct cccagcccat
      361 cacgtcctcc gtatcgggcc aggtggtgct gtagcttccg aggatctccg gtccggtcga
      421 gtctccgagc agcacggcgg ccgcaccgtc ggcgaagagg ctagtggaga tcaggttgct
      481 ccgcgagcga tcgccgcgct ggaaggtgag gccggagagc tcgacggcga cgaggagggc
      541 gagcttgtcg gggtagagac gggcgtgctc cgaggcgcgg gcaagccccg acgcgccggc
      601 ggagcagcct aggccccaga taggaacgcg gcgcgtgtgc tccgagaggc cgagcctgaa
      661 gatgagcttg gagtcgaggg atggagtaga gatacccgtg gtagagacga agaagatcgc
      721 gcccacatcc tccggtttgg ccccgatcct atcgagtgcc ttgctcgctg ccttctcgga
      781 gaggtcgagg gcgtgctcga cgtacagggc gttcttctcg gggaagccgt ggtcccgctc
      841 gaaccactcc accggcacgc agaagtaacg gctatcgacg ttgacgttgt cgaagattgg
      901 gagcaggcgc tcgatgtcgc cgtcccggcg cgagaacatg ccccgggcga acgtcttcgc
      961 ctcctcctgc ccgatgcgat gcggcggcag ggccgtggcg acagaaagga tccttggggc
     1021 gtctccgatc aattcttccc cgggcttgcc tgccgggaca cgccgggttc cgtaccggcc
     1081 ccggcgtctg gttccggtgt ctcgcaggtg ttgcggaaca cgtactgtga gaggcgtttg
     1141 cgctggcggc tggaccagtc gatggtgggc ctgcgttgct cggtgggaac gtatcccagc
     1201 ctgtagaccg ccatgagctc caggtcttcg ggcaccttga acaacctctt aaggcgctcc
     1261 cagttctcgg ggatctccat cggggtggag acgaactgga tgcccatacc gagctcggtg
     1321 gtggtgagcc agatgttctc gacggcggcc cccatcccga agaccgagta gaagccggac
     1381 agttcgcctg gcctgtactc cgtcttgtca aggaggacgg ccagcaggag cggtgagccg
     1441 gcgactagct tcctgttgtc ctccccaagc gttttcggga cgccgaagcg gttcatcacc
     1501 gcctggcccg tggaggagaa gatctggcgc cggaaaggct ttagaaccgc gggcatctgg
     1561 tcgaagtgaa tgccgtcacg cctctgttcc atctcggcct cggagaaacg gaaatacggc
     1621 ctgtagcgct tccagaaggt gccttcctcg aggaggcgcg tcatgctgtc cccgcttatc
     1681 cgggcgacct cctcgatggt ctctcggtcg tcagatagca cgaaacgcca gggctggctg
     1741 ttgaagtgtg agggtgccat ggcggcgacc tccaccagca gccgctggtg ctcgggtctc
     1801 acggggtcgg gcaggaaggg cccgttcgtc gtcttgcgcc tcttgaggac gtcaaggaaa
     1861 tccaaatcag cctcctacaa catccatacg cagccggtgg cgtagaggat cagggcgccg
     1921 ctagcaagcg cggcgtgccc aggcgtcccg gcccttaccc gcggtagcac ggtgaagagc
     1981 atgaggacgg gcagaagcac ccaacgataa ggcgccccct gcaagaatcc cacaccggcg
     2041 gcggcggcgg ccgatacgaa ggccgcgaag aacagggcat ggtggagcca gcggaaaggc
     2101 ttcgtatcca cgacgcgaaa ctggaccagg atgccaagaa cgaagttggc aacgaagaag
     2161 acagtggcga cgtagaagat caatgctcgc tcctcgggct catctttgag ggttatagca
     2221 caggcgccgc cggcgacggt aagagcagac gccgccggcc ggcaccagaa ggccacacgg
     2281 cggcagagtc ggcgacggac tctgatgcta agatttgtac ttcgatggat ctttaatttt
     2341 gtggggggct agatggctga aaagcgcccg gaagcttata tcacggggat aggggtggtc
     2401 acccctatag gggtcgggcg cgagaagttc tggggctcgc tcctggccgg cgagagcggg
     2461 gccgggccaa ttaccctctt cgacccggag ggctacgacg tgcggatcgc agccgagtgc
     2521 tcggatttcg accctgagag cttcatggac aggaaggtgg cggcccgcac cgaccgcttc
     2581 gggcagatgg gcctcgcctc cgcgaagctg gcgctcgagg acgcggacgc ctgggacgtg
     2641 ctgaaccagg agccagagag ggtcggcctg atactcggga gcggactcgg gggcgtgacc
     2701 agcatggaga gcacccaggc gaccatagac agcaagggcc ctgggcgcgt caacccgttt
     2761 gccgttacca agatcatgcc aaactcagcg gctgcccacg tgggtatcca gctcggcgtg
     2821 cagggtccct ccatcgcggg ggcgctcgcc tgcacctgcg gcaccgacat gatggcgctc
     2881 gggtacgacc tgattcggcg cggcgacgcc gacgtggtgt tgtgcggcgc ctccgaggca
     2941 gccctgacgc ccgtgatagt ggcaggcttt atcgcgatgc gcgccatgag ccggcgcaac
     3001 gacgacccgg agggggcctg tcgtccctac gacgtggacc acgccggatt ccttatcggg
     3061 gaggcctccg ccgtgatggt gctggagagc gccgagtcga tggagcgccg gggctccgag
     3121 ccctacgcca agatcaccgg cgtgggacgt acgacggacg cctacaacct caccgatccc
     3181 gaccctggcg gccacggcat caggcgtgcc atgcagctcg ccgtggccga cgccggcctt
     3241 gagggggagc agatcggcta catcaacccc catggcaccg gcaccgtggc tggcgacgca
     3301 cccgagtccc aggccatggc cgccattaac cccgacgtca aggtatccgc gaccaagtcc
     3361 accctcggcc actcccttgg ggcgagcggc gccctggaga cggcaatctg cgccctcgcc
     3421 atgaaggagc ggacgattcc tcccatgaga aacctcacgg aggtcgccga aggctgcgcg
     3481 gacctggact acgtggtcga cgagccacgc gaggtgcccg acttgcaggc ggccatctgc
     3541 gccaacctgg gcgttggggg ccacaacgtc gctatatccc tggagcgggc ttgagcgacg
     3601 tctatacggt gccagagggt ctgcccgagc cgacagacga tggcgcctgc gatcacctgc
     3661 ccggcctgga ccttcctacg gtatccctga cctcgacctc gggtgacgag gtcgacctct
     3721 ccgaactggc cggcaccacc gtggtgtact gctacccgct gacggggagg cctgaccggg
     3781 aactgccggg agggtgggat gagatcccgg gcgcccgcgg ctgcacaccc cagtcgtgtg
     3841 cctttcgcga ccaccacgcc gagctcgaag atctcggtgc gagggtcttc ggcctgagca
     3901 cccagacccc cgactaccag cgggaggccg cgcagaggct tcaccttccg tttccgcttc
     3961 tcagcgacca gaacctggag tttgcggaag ccctggacct tcccacgttc ggggccgaag
     4021 ggatgaccct cgtcaagcgc gtgaccctga tcgtcgaaga cggggagatc cggaaggtct
     4081 tctatccagt ttttccaccg gggagaaatg ccgaagaggt gatcctctgg ctctcccaac
     4141 aggcaacaag ctagagagga cgtgcatatg aaccccgtcc cgacggacgt actgcaagag
     4201 aagctcacgg aactggaggg aaccctggcc ccctacggga gcgccctagt cgctttttcg
     4261 ggcggtgttg actcctcact ggcactggcc gtcgcggcgc gggcgctgcc gaggcacagg
     4321 gtgctggccg tcacgtccaa caacgagact tatctgccgt cggagttgga cctcgctcgc
     4381 gagttcgccg actccctcgg ggtagagcac ctcgtcgtga acaccaggga gctcgacaac
     4441 cccaactacg ccagcaaccc aacaaaccgt tgctacttct gcaagagcac gctctactcc
     4501 gacctggcgc ggctcgccag ggagaagggc tacgcttgcg tcgtggacgg ggctaacaag
     4561 gacgacgaag gcgactaccg cccgggcagg aaggcggcga aggagctcgg ggtgatctcc
     4621 gtgctctccg cggcccgcat gtccaaagag gaggttagag agctcgcgaa acacctcggg
     4681 cttcccacgt gggacaagcc ggcgctcgcc tgcctctcca gccgctttcc ctacgggcag
     4741 gagataacgc cggagaaact cacccaggtg gcgcgcgccg aggagttcct gcgcagagaa
     4801 gggttccagc aggtccgcgt caggcaccac ggggagatcg cccgcctcga ggttggccct
     4861 ggtgagctgg agcgggcctt cgccatgcgg gagaagatct ccgccgagct tctcgacgct
     4921 ggctttctct acgtcaccct ggacctcgcc gggtacaaat ccggcagcct caacagggcg
     4981 ctgaagaaag acaagaaggc tttgcccctc atctcgacct gagcatggac aacgtagaag
     5041 gaccgctgct agccctcacc atcgtcttga tggcggtcgg cctcgtcgga tgcgtcgtgc
     5101 ccggcctgcc cggcgtcacg ctcatctttc tctcggcgct gctctacgcc atcctcacgg
     5161 acttcgagac ggtaggaacc ctcgtcctgg tgatcctctt cgtgctcgcc gcgctcgcct
     5221 tcctcaccga cttcgtcgcg acctcctacg gcgcgcggcg cttcggggcc agcaactggg
     5281 gcaccgtggg tggcgcagtg gggggcatcg tcggtgccgt ggcggggctg gtattcgccg
     5341 gtgtcgggtc cctgttcggc ctcatcctgg gctccattgc gggtgtcttc ataggggaat
     5401 acctgcgccg ccaacgccgc ggcgatcagg aacgacagag caccgactgg cgacgtgcct
     5461 cccgggcggc gggcggcgtg atcgtcggct atcttgcctc agcggtgatc cagggtcttc
     5521 tggggctagc gagcatcatc atct
//
LOCUS       DBNGVD010000221         5461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2232219, whole genome shotgun sequence.
ACCESSION   DBNGVD010000221 DBNGVD010000000
VERSION     DBNGVD010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2232219"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="AC16CM_06190"
     CDS             complement(<1..871)
                     /locus_tag="AC16CM_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3139454.1"
                     /translation="MFPTRVLLAFDGSTGSRDAAQMAVSLSNALDSPLYVVLVENLTP
                     TGRAIPEEPYFGADAGYRSRGFSESEVRDEAERQAKQIREQGGRIAGLHHRIGHPVAE
                     ILHVAEETDAGLVVVGSRGLGRLRRKLLGSVSEGVVRHAHCAVLLVRGGCGELGPAVP
                     LVLAVDGSRETAPATEAAAEISRATGCPVHLVYALQIERYRPYPGPETWEGWEEDLAR
                     AELDATRWLQGHAEDLKSKGARTVEAHLFRGEPDGAILRLVDTLGAGLVVLGSRGLGG
                     IRRAFLGSVSESVM"
     gene            complement(955..1938)
                     /locus_tag="AC16CM_06195"
     CDS             complement(955..1938)
                     /locus_tag="AC16CM_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3139455.1"
                     /translation="MLSSVLLATDGSADAAVASEVAASLCDKLGAELHVVHAWRTPAM
                     IEAAPVPIEPFAEDARRILDAEVDRAARGGARRIQAHQEEGSPIRVILGLADVLGSDL
                     IVMGSRSRGPLLRLALGSVSEGVLYRAARPVLVARGGENAWPPRRIVVGDDGSAEAGR
                     AGELAADIGDLFGARTTLVRASDNASRSHGSSDYGSNTSERVVVDFQREDRMDLRLRA
                     EVLGLKVGTEPETRLVGGDAASVILEAAREDPATLVAVGARGLGGIRRALLGGVSNKV
                     LRASEGPVLVYATPAKMPDKGLQDRMDQGERRSPASPRRSGRPATSLWSLL"
     gene            complement(2014..2490)
                     /locus_tag="AC16CM_06200"
     CDS             complement(2014..2490)
                     /locus_tag="AC16CM_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3139456.1"
                     /translation="MKTLVVYDTQFGNTERLARNIAEALHEHGEARVAPVAGVSGLHG
                     GEVDLLVVGGPTQGHGLPGAMESFLRNAPAGTMRGTRALAFDTRYRGPRLLQGSAAHH
                     IAGQLRRLGCQLLAPPESFFVTGEEGPLEPGEVDRARGWALHALGRAEQASAHRAH"
     gene            complement(2514..3011)
                     /locus_tag="AC16CM_06205"
     CDS             complement(2514..3011)
                     /locus_tag="AC16CM_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3139457.1"
                     /translation="MNGGSKDGNPGKILVATDGSHDAASAVHRAAGMARYFGAELFLV
                     HVVPVSSPYRMSGGDFDEGPSLYEEDTERARALLDEQARMAREDGVQISGTYLKSGEP
                     AAEVVALAETMGADLIVAGGRGMSPMRRPIGSVSGSIAAHAHCPVLVVRDTNGGRNGA
                     VDPGA"
     gene            complement(3011..5461)
                     /gene="ppsA"
                     /locus_tag="AC16CM_06210"
     CDS             complement(3011..5461)
                     /gene="ppsA"
                     /locus_tag="AC16CM_06210"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR3139458.1"
                     /translation="MVLGTRWVRHFSEVGIEDVPLVGGKNASLGEMTRELGTLGIRVP
                     EGFALTADAYRHFLESNGLVLPVRDALDGLEPGDVEDLGRKSRLIRDLVLGAEIPSEV
                     KDEVLRGYEELSSRYGVDHADVAVRSSATAEDLPTASFAGQQESYLNVHGEAQLLESV
                     RKCFASLFTPRAISYREDMGFDHFGVALSVGIQKMVRSDLASSGVIFTLDTESGFPDV
                     VFVTSTWGLGENIVQGRVIPDGFYVHKPTLGEGYAPLIRKRLGTKEFKLVYDSSGHRL
                     VENVPTSPQERATFSVSDEDVLKLARWAVDIEEHYGQKRGEQTFMDIEWAKDGITGDL
                     FILQARPETVHGAGRVPTLRMHRLKERGRMLAEGLAVGNTVATGPARVLEDPSEIEAF
                     RHGEVLVTEITDPDWEPIMKVASAIVTERGGRTSHAAIVARELGIAAVVGTGDATQRI
                     RTGVPVTVSCCEGEVGHVYAGVLDYEVTEVDPGAVGRPSTKIMMNVANPERVFELAQI
                     PNDGVGLARMEFVFAGWVGVHPLALTRYASLPPGIKLEVSRLTRGYEDKSEFFVDRLS
                     QGIGTLAAAFWPRPVVLRFSDFKTNEYAHLLGGELFEPEEENPMLGWRGSSRYYHPDY
                     KEGFMLEVRAVKRVREVFGLKNLVVMLPFCRTPEEGERVLEVMREGGLARGRDGLEVY
                     VMAEIPSNVVLASEFANLFDGFSIGSNDLTQLVLGVDRDSERVAPLFDERNEAVKRMC
                     ATLIEEAHRAGRKVGICGQAPSDYPDFATFLVGLGIDSISLSPDAVVPTTLRILKEEG
                     SPGGYTEDDTTTKRREDR"
BASE COUNT          888 a         1949 c         1673 g          951 t
ORIGIN      
        1 gcatcaccga ctccgagacg ctgccgagaa atgcccgccg gatgcccccg agcccccggc
       61 tgccaagcac gacaagcccg gcccccaggg tgtcgacgag gcgcaatatt gccccatctg
      121 gctctccacg aaacaggtgg gcttcgaccg ttctggctcc tttcgacttc aggtcttcgg
      181 cgtgaccctg caaccacctt gtcgcgtcca gctccgcccg cgcgaggtct tcctcccatc
      241 cctcccacgt ctcgggtccc gggtagggcc tgtagcgctc tatctgtagg gcgtagacga
      301 ggtgaacggg gcagccggtc gcgcgcgaga tctccgccgc tgcctccgtt gcaggcgccg
      361 tctccctcga accgtcaacg gcgagaacta gcggtacggc aggcccaagc tccccgcatc
      421 caccccgcac gaggagcacg gcgcaatggg cgtggcggac tacgccctct gatacgctac
      481 ccagtagctt tcgcctcagt cggcccagac cccggcttcc gaccaccacg agcccggcgt
      541 cggtctcctc tgccacgtgc aagatctcgg cgaccggatg gccgatccga tgatgtaagc
      601 ccgcgattcg accgccctgt tctcttattt gcttcgcctg ccgttcggct tcgtccctga
      661 cctcgctctc cgagaacccg cgcgaccggt acccggcatc ggccccgaaa tacggttcct
      721 cgggaatcgc tcttccggtt ggcgtcaagt tctcgaccag aaccacgtag aggggggagt
      781 cgagggcgtt cgagagcgac accgccatct gcgcggcgtc ccgagacccc gtagatccat
      841 cgaaggccag caacactctc gttggaaaca cgtgcccgcc cgcctaaccg tacccgtcga
      901 cgtacacgcc gactggcaac cccggccagg cgtcgcacgg ccgccaggta gtgatcacag
      961 cagagaccat aaagatgttg ccggccgacc cgaccggcgc gggctagccg gtgatcttct
     1021 ttctccctgg tccatccggt cctggaggcc cttgtcaggc atcttcgcgg gcgtcgcgta
     1081 gaccaggacg gggccttcgg aggcccgcag gaccttgttc gaaacgccgc cgaggagggc
     1141 cctccttatc ccacccagtc ccctggcccc gacggcaacc agcgtggccg ggtcctctct
     1201 cgcggcttcg aggatcacgc tcgcggcgtc gccccccact agtcgggtct ccggttccgt
     1261 acccaccttc aggcccaata cttcggccct gagcctaaga tccatccggt cctcccgctg
     1321 aaaatccact accactcgct cggaggtgtt cgacccgtag tcagatgaac cgtgcgatct
     1381 cgacgcgtta tcggaagccc gcacgagcgt agttctggcc ccgaagagat caccgatatc
     1441 ggcggcgagc tcaccggccc tgcccgcctc tgccgagcca tcgtcgccga ccacgatcct
     1501 tcggggtggc cacgcatttt ccccgccgcg cgccaccagc accgggcggg cggcacgata
     1561 gagcacccct tcggagacgc taccgagggc gaggcgcagc agcggccccc tactacggct
     1621 gcccatcact atgaggtccg acccaagcac gtcggccagg ccgagtatga ccctgatcgg
     1681 ggagccctct tcctggtgcg cctggattct cctggcgcca ccacgcgcgg cgcgatccac
     1741 ctcggcgtcc agaattcttc tcgcgtcctc agcgaatggt tcgatcggca cgggtgcggc
     1801 ctctatcatg gcgggggtgc gccaggcgtg gacgacatgg agttcggccc ccaacttgtc
     1861 gcacaagctt gccgccacct ctgaggctac cgcggcgtcg gccgatccat ctgtcgccag
     1921 gagtacgctg ctcaacatcg ttcgggaacc tcttgacttt ccggagggta gacaaaccag
     1981 cagggtgcgg gccccaacag aacctggtct ccttcagtgg gcacggtgcg cggaagcctg
     2041 ctccgccctc ccgagggcgt gaagggccca acccctggcc cggtcaacct cgccgggctc
     2101 cagggggcct tcctcgcccg tcacgaagaa gctctccggc ggggcaagca gttggcagcc
     2161 cagacggcgc agttgccccg ctatgtggtg cgctgcggaa ccctgaagga ggcgtggtcc
     2221 gcggtatctg gtgtcgaagg ccagggcacg cgtcccccgc atcgtccccg cgggggcgtt
     2281 acgcaggaag ctctccatgg cgcctggaag cccgtggccc tgggtgggac caccgaccac
     2341 gaggagatcc acctcccccc cgtgtaatcc ggagaccccg gcgacagggg ccacgcgcgc
     2401 ctcaccatgc tcgtgaaggg cctctgcgat attgcgggcc agacgctccg tgttgccgaa
     2461 ttgcgtgtcg tagacgacca gcgtcttcat gcgttcgctc ctctcctctt gctttacgcc
     2521 ccgggatcga ccgccccgtt tcggccgccg ttcgtgtccc ttaccacgag cacggggcag
     2581 tgggcgtggg cggcgatgct gcctgagacg cttccgatgg gacgcctcat cgggctcatg
     2641 ccccggccgc cggccacgat caggtcagca cccatcgtct cggccagggc gaccacctcg
     2701 gcggctggct caccggactt caagtaggtg cccgagattt gcaccccgtc ctcccgcgcc
     2761 atccgtgcct gctcatcgag caacgccctg gcccgttccg tgtcctcctc gtagaggctc
     2821 gggccctcgt cgaaatctcc gccggacatt ctgtaggggg aggaaacggg cacaacgtgg
     2881 accaggaaga gctcggcgcc aaagtaccgg gccatcccgg ccgcccggtg cacggctgac
     2941 gcagcgtcgt gggaaccgtc ggtggccacg agtatctttc cggggtttcc gtctttcgag
     3001 ccgccgttca tcaccggtcc tctcttctct tggttgtcgt gtcgtcctcg gtgtacccgc
     3061 cgggagagcc ttcctccttc agtattctca gggtggttgg caccacggcg tctgggctca
     3121 ggctgatgga atctatgcca agcccgacca ggaacgtcgc gaagtccggg tagtctgacg
     3181 gggcctgccc gcatataccg accttgcgac ccgcacggtg ggcctcttct atgagggtgg
     3241 cgcacatcct ctttacagcc tcgttccgct cgtcgaagag cggcgccacc ctctcggaat
     3301 ccctgtccac cccgaggaca agctgggtga ggtcgttcga tccgatggag aagccgtcga
     3361 agaggttggc gaactcgctg gcgagcacga cgtttgaggg aatctcggcc atgacgtaga
     3421 cctcgagccc gtcccggccc ctggcgaggc cgccctcgcg catgacctcg agcaccctct
     3481 cgccctcctc cggggtgcgg cagaaaggta gcatgaccac gaggttcttg aggccgaaga
     3541 cctcgcgcac ccgcttgacg gcgcggacct cgagcatgaa accttccttg tagtcggggt
     3601 ggtagtaacg gctcgacccg cgccagccga gcatcgggtt ctcctcctcg ggctcgaaga
     3661 gctccccgcc gaggaggtgg gcgtactcgt tggtcttgaa atccgagaag cgcagcacga
     3721 cgggccgggg ccagaaagcc gcggcgagcg tgcctatccc ctgcgagagc ctgtccacaa
     3781 agaactcgct cttgtcctcg tagccccggg tcagacgcga gacctcgagc ttgattccgg
     3841 gcgggagcga ggcgtaacgg gttagcgcca ggggatgcac ccctacccat ccggcaaaga
     3901 cgaactccat gcgcgcgagg ccgacgccgt cgttcgggat ctgggccagc tcgaagaccc
     3961 gctctgggtt ggccacgttc atcatgatct tcgtgctcgg tcggcccacc gccccggggt
     4021 ccacctcggt gacctcgtag tccagaacgc ccgcgtagac gtggccgacc tccccctcgc
     4081 agcaggagac cgtgacgggc accccggtcc ggatcctctg tgtcgcgtcc cccgtgccaa
     4141 ccacggccgc aatcccaagt tcacgggcga cgatcgccgc atgtgaggta cgccctccgc
     4201 gctccgtcac gatggcggag gcgaccttca tgatcggctc ccagtcgggg tctgttatct
     4261 ccgtgaccag cacctcgccg tgacggaaag cctcgatctc gctcgggtcc tcgagcaccc
     4321 gggccgggcc cgtggcaacc gtattgccca ccgcgagccc ctccgcaagc atccttccgc
     4381 gctccttgag ccgatgcatg cgcagcgtcg gcaccctgcc ggcaccgtgc acggtctccg
     4441 ggcgggcctg caggatgaat aggtcgcccg tgatgccgtc cttggcccac tctatgtcca
     4501 tgaacgtctg ctcgccacgc ttctggccgt agtgctcctc gatgtcgacg gcccagcgcg
     4561 ccagcttcag cacgtcctcg tccgagacgg agaaggtggc tcgctcctgg ggggaggttg
     4621 gcacgttctc cacgagacgg tgccccgatg agtcgtagac gagcttgaat tccttggtcc
     4681 cgagcctctt tcgaattagc ggggcgtagc cctcaccgag ggtgggtttg tgtacgtaga
     4741 agccatcggg gatgacgcgg ccctgcacta tgttctcgcc gagcccccag gtcgaggtca
     4801 cgaaaacgac atcagggaac cccgactccg tatccagggt gaagataacg ccggaggacg
     4861 cgaggtccga gcgcaccatc ttctgtatgc ccacggagag ggccaccccg aaatggtcga
     4921 agcccatgtc ctcgcggtag ctgatggccc ttggggtgaa gaggctcgcg aagcacttcc
     4981 tgaccgactc gaggagctgc gcctccccgt gaacgttcag gtagctctcc tgctgtcccg
     5041 cgaagctcgc ggtgggcaga tcctccgcgg tcgccgaaga acgaacggcc acgtcggcgt
     5101 ggtcaacccc gtagcgggat gagagctcct catagccgcg gagtacctcg tctttcacct
     5161 cggacggtat ctcggcgcca agcacgaggt cccggatgag gcggctcttg cgtcccaggt
     5221 cctcgacgtc gccgggctcg aggccgtcca gtgcatcacg aacgggaagg accagcccgt
     5281 tcgactcaag aaagtgccgg taggcgtcgg ccgtcagggc gaacccctcg ggtaccctga
     5341 tgccgagcgt cccgagctcc cgggtcatct cccccagcga ggcgttcttg ccccccacga
     5401 gcgggacgtc ttcgatcccg acctccgaaa aatgcctgac ccagcgcgtc ccgagcacca
     5461 t
//
LOCUS       DBNGVD010000222         3398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3325343, whole genome shotgun sequence.
ACCESSION   DBNGVD010000222 DBNGVD010000000
VERSION     DBNGVD010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3325343"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16CM_06215"
     CDS             complement(<1..548)
                     /locus_tag="AC16CM_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3139459.1"
                     /translation="MDAVQKAGNGHPGTAMALAPLAYTIFTKFMRHNPENPGWKDRDR
                     FILSAGHASILQYSMLHLTGYDLPLEQIERFRQTGSITPGHPEHFLTPGVETTTGPLG
                     QGFANGIGMAMAERFLADRYNRIGHDIVDHRVFAICSDGDLMEGVANEAASLAGQNAL
                     GKLVYYYDDNRITIDGTTAISF"
     gene            complement(960..1748)
                     /locus_tag="AC16CM_06220"
     CDS             complement(960..1748)
                     /locus_tag="AC16CM_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139460.1"
                     /translation="MATIANIDKTARTAREMAEAQRDTFEAVAENFAAAQRRSMGLAE
                     GGLEFVRLQEQNARATQEFFANGVRLLQLQQRNAEFVQGWMSEAVEVVREQAEQNART
                     VEAFARTASKQQESLRSLTRTWTGAYGDFFSPFTYFQEGVKTFQRVAQQGLAATEQVA
                     RQGLKVSEEATRQGLRVAEEAAEQTDKALRQGEKAAHEVELRTAVSSALGTPNYDELS
                     VAEVSRRLDGLSTDQLHKVREFEKNNKNRETLIEQIDRKIRANS"
     gene            2003..2251
                     /locus_tag="AC16CM_06225"
     CDS             2003..2251
                     /locus_tag="AC16CM_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3139461.1"
                     /translation="MPRGTFRWFDDARSYGCISPNDGGEDIFVRNTGISGEGFETIDE
                     GDEVSLQVTLVRKGMQACDVSEVLAAAWGRRPRSGVLS"
     gene            2268..2873
                     /locus_tag="AC16CM_06230"
     CDS             2268..2873
                     /locus_tag="AC16CM_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139462.1"
                     /translation="MYPDHITQEDFVKRSAWAEISLGYARAHHQDHLARLLGAVREDV
                     EFEGTLVSLPPGEHLRSAREGSRGEIARKLARGMESEEDAKRRKREEENARNRLAAWK
                     LFVEAEFRGLRLRQEGQLGRQLGEPLAGESAAALERLAEEDRRQALSGLVALMSNGRT
                     AYKRVEDLREGDMPARAAADRLRTTWLKERRDGWLVRGIGA"
BASE COUNT          564 a         1051 c         1127 g          656 t
ORIGIN      
        1 tcaaaggaga tggcggtcgt gccgtcgatg gttatgcggt tgtcgtcata gtagtagacg
       61 agcttgccga gggcgttctg cccggccagc gaggcggcct cgtttgcgac gccctccatc
      121 aggtcgccgt cggaacagat ggcgaagacc ctgtggtcca cgatatcgtg cccgatgcgg
      181 ttgtaccggt cggccaggaa gcgctcggcc atggccatcc cgatgccgtt ggcgaagccc
      241 tgcccgagcg gccccgtagt cgtctcgacg ccgggcgtca ggaagtgctc ggggtggcca
      301 ggggtgatcg agcccgtctg acggaaacgc tcgatctgct cgagcggcag gtcgtagccg
      361 gtgaggtgta gcatggagta ctgcaggatc gaggcgtgcc cggcggagag gatgaagcgg
      421 tccctgtcct tccaacctgg gttctccggg ttgtgacgca taaacttcgt gaagatggtg
      481 taggccagcg gggccaacgc catcgccgta ccgggatggc cgttgccggc tttttgtacg
      541 gcgtccatcg ccagcgtcct gaccgtatca atggaacgac gcgccacgtc ctgctgggtc
      601 ttgaggtcca cgatccctcc cactttgttc gttttcacca cggcgccagg ccccgacgca
      661 taccacgccc ggacccgacc gcaagaaccc gacgccggac gtcgcgctct cggcgctatt
      721 gttacccgat aggtaccttc tgtaagccac acccctctcg tcccaggtgg tcctcgcgcc
      781 gggaccgtct accccagccc cagctacact cccgctaccg ttcgcgttac cccccgccgt
      841 gccggccagt acggaccata caggattacg ggatcaggta cgagcagaaa cgcaggcaag
      901 agaagaggcc gggattcgca tcccggcctc cggcgtaact ctttgtggga agaacccctc
      961 taggagttgg cccttatctt gcggtcgatc tgctctatga gggtctcacg gttcttgttg
     1021 ttcttctcga actcacgaac cttatggagc tgatcggtgg agaggccgtc gagccgcctg
     1081 gaaacctcgg ctacggacaa ctcgtcgtag ttgggggtgc caagggcgct cgacacggcg
     1141 gtcctgagct caacctcgtg ggcggccttc tcaccctggc gaagggcctt gtcggtctgc
     1201 tcggcggctt cctcggccac gcgaagtccc tgacgggttg cctcctccga gaccttgagt
     1261 ccctgcctcg ctacctgctc cgtcgccgcg agcccctgct gggcgacccg ctggaaggtc
     1321 ttgacaccct cctggaagta ggtgaagggg ctgaagaaat cgccgtaagc ccccgtccag
     1381 gtgcgggtga gggaccgcag gctctcctgc tgcttgctcg cggtccgcgc gaaggcctcg
     1441 accgtacgag cattctgctc ggcctgctcg cgtacgacct cgacggcctc gctcatccaa
     1501 ccctgcacga actcggcgtt gcgctgctgc agctggagaa gcctcacccc gttggcgaag
     1561 aactcctggg tcgccctggc gttctgctcc tgtagacgca cgaactcgag cccaccctcg
     1621 gcgagcccca tgctccgccg ctgggcggca gcaaagttct cggcaacggc ctcgaacgtg
     1681 tcgcgctggg cttccgccat ctcccgggcg gtgcgcgccg tcttgtctat gttggctatg
     1741 gtagccatgc tggtacctcc tgtagctggt aactggttgc agctatgttc ttaccacaca
     1801 tatgttctgt gtaaacctca gatcggtggg aggccctttc tcttctcggc ctgttcgcgg
     1861 gtttacgggg ggtctcgggc gggcccgtgg tacaattggg ggaatcatag ccgtgggttt
     1921 tatggttagc tgagcgccgc cctcgacacg aggagcggct tttttgttgc ggtgtagccc
     1981 acggaggagg cggaattcag gcatgcccag aggaacgttc agatggttcg acgacgccag
     2041 gtcctacggc tgcatctccc ctaacgatgg tggtgaggac atcttcgtac gcaacacggg
     2101 aatatccgga gagggcttcg agaccatcga cgagggtgac gaggtctctc tccaggtgac
     2161 cctggtacgc aagggcatgc aggcctgcga tgtatcggag gtcctggcgg ccgcatgggg
     2221 acggcgcccc cgatctggcg tcctcagctg accgaaagga gcgtgccttg tacccggatc
     2281 acattacgca agaggatttc gtgaagaggt cggcctgggc agagatctcg ctgggatacg
     2341 cccgcgcgca ccatcaggat cacctggcgc ggctgctggg ggcggtcagg gaagacgtcg
     2401 agttcgaggg gacgctggtc tccctgccgc ctggggagca cttgcgctcg gccagggagg
     2461 gctctcgggg agagatcgcg cgcaagcttg ctcgcggcat ggagagcgag gaagacgcga
     2521 agcggcgaaa gcgggaagag gagaacgctc gcaaccggct cgcagcctgg aaactgttcg
     2581 tcgaagcgga gtttcggggt ctgaggctgc gccaggaggg tcagctcggc cggcagctgg
     2641 gtgagcctct cgcaggggag tcggcggccg cgctcgaacg gctggccgaa gaggaccggc
     2701 ggcaggcgct cagcgggctg gtcgcgctga tgagcaacgg acggacggcc tacaagcggg
     2761 tcgaggacct ccgcgaaggg gatatgccgg ccagggccgc ggcggaccgg ctgcgtacta
     2821 cctggctcaa ggagcgccga gacgggtggc tggtacgggg cataggcgcc tgagaagagt
     2881 cttccctttc ggcccagagg ctcctcgata tgagacgttc agggccgtct ggcgggttct
     2941 ccaggttgcc cggttggcaa ggcttcctgg tccacgacac gtggccacca cgctcgaagc
     3001 acgttcgcac gtgctttcca acatgcagca gagggccgcg cgagcactcg aagaggcccc
     3061 ggagtagcgt cctggcatgg ttgctacacc ggggttccgg ctctcctccg ggacctgggt
     3121 actactgccc agtaccgccg tgtccgcaat tgcggctcaa gtaagccact ttctcacgtc
     3181 ggctctggac gtcgatccgc tgcgtatcgt ccagtaccgt ccgggtaggc gttcgtcttg
     3241 acagaggttc ccgtcactcg gtcgctctca gcactgcctt ccaggagtta tgaagcgccg
     3301 agaggaggag cagtacggta aggctccctt agccgccacg gttatggtct tgcagctgtc
     3361 cccacgggtg cttgtgagtt cgccaatgca ttatttgg
//
LOCUS       DBNGVD010000223         1904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4237103, whole genome shotgun sequence.
ACCESSION   DBNGVD010000223 DBNGVD010000000
VERSION     DBNGVD010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4237103"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16CM_06235"
     CDS             complement(<1..352)
                     /locus_tag="AC16CM_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3139463.1"
                     /translation="MPENEREPVGLFGVPMDLGQDRRGVDMGPSAIRYARIEDALEEL
                     GHRVTDLGNAGVPIPEMVDSGEHVKHLDAVKRVCEEVAQRATSIVSDGLFPIFLGGDH
                     SISIGTVSGVARSAS"
     gene            complement(345..1895)
                     /locus_tag="AC16CM_06240"
     CDS             complement(345..1895)
                     /locus_tag="AC16CM_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3139464.1"
                     /translation="MNSTGLNHSFAAAAGTPYAADAAAETYRAGGNAADAAVAAAAAV
                     GVTEPLMSSIGGGGFALVRDPSGGAELIDYYDTMPGKGLPASSFGAGGAPQTMILKYG
                     AGVRSIVGGASSAVPGALRGWEKLLERHGRLTLREALAPAIRLAREGFDLCRTSGLWF
                     QVAEEVLQLTEETRKHYYKGDRVYLEGEEMRFPELADTLQAVGEDGVGLFYEGELARR
                     ISSYMLEMGGIITEQDLAEYEAVIREPMSVSYGAGAMYTNGPPSAGGSTLAQMLKVVS
                     AYDLVAMPDEEYARLVAGAMRLALEDRDNQYLDGAKNSEVARRLTGEEYAAEQRQRIF
                     SSFGSPHTTHLSCVDAEGLAISITASMGYGSGLVVPGTGIPMNNTLGEPELNPKGFHA
                     LDPGERLVSNMSPTVVSSEEGGLISLGSPGASRIPTAILQTVMNVVDFGMSLEEAVLA
                     PRFHAEDDLFAYEAGARTADLEGYERVLSYETPNMFFGGVNAVRRTPSGRFEAAADPR
                     RSGGTTFA"
BASE COUNT          310 a          651 c          619 g          324 t
ORIGIN      
        1 ccgaggcgga gcgagcgacc ccagacacgg tcccgatgga gatggagtgg tccccgccca
       61 ggaagatggg aaagagtcca tcggagacga tggacgtggc cctctgggcg acctcctcgc
      121 aaacgcgctt cacagcgtcg agatgcttga cgtgttcacc cgaatcgacc atttcgggga
      181 tgggcacccc cgcgttcccg aggtcggtga ccctgtggcc gagctcctca agcgcatcct
      241 cgatgcgggc gtagcgtatg gcgctcggtc ccatgtccac gccccttcgg tcctggccca
      301 ggtccatggg cacaccgaag aggccgaccg gctcgcgctc gttctcaggc aaaggtggtc
      361 cctcccgaac gtcgagggtc ggccgccgcc tcgaagcgcc ctgagggcgt cctcctgacg
      421 gcgttgaccc cgccgaagaa catgttgggg gtctcgtagc tgagcacccg ctcgtagcct
      481 tcgaggtcgg ccgtgcgggc accggcctcg taagcgaaga ggtcgtcctc ggcgtggaag
      541 cgcggggcga ggaccgcctc ctccagcgac atcccgaaat caaccacgtt catgacggtc
      601 tgcaggatgg ccgtcggtat acgcgaggcg cctggtgagc cgagcgagat caggcccccc
      661 tcctccgagg agacgacggt ggggctcatg ttcgagacca ggcgctcccc cggatcaagg
      721 gcgtgaaagc ccttggggtt cagctcgggc tcgcccagcg tgttgttcat cgggatgccg
      781 gtgcctggca cgacaagccc tgatccgtag cccatgctgg cggtgattga gatggctagg
      841 ccctcggcgt ccacgcagga gaggtgcgtg gtgtgcgggg atccgaagga ggagaagatc
      901 ctctgccgtt gctcggcggc gtactcctcc cccgtcaacc gccgggcgac ctcgctgttt
      961 tttgcgccgt cgaggtactg attgtcgcgg tcctcgagcg ccagtctcat ggccccggcg
     1021 acgagtcgcg cgtattcctc gtccggcatg gccaccaggt cgtacgctga gacgaccttg
     1081 agcatctggg cgagggtgga gccaccggcg gagggaggcc cgttggtgta catggccccc
     1141 gcaccgtaac tgacgctcat cggctccctg attactgcct cgtactccgc gagatcctgc
     1201 tcggtaatga tgccgcccat ctcgagcatg taggacgaaa tgcggcgtgc cagctccccc
     1261 tcgtagaaga gccctacccc gtcttcccct accgcctgca gcgtgtcggc gagctcgggg
     1321 aaccgcatct cttcaccctc caggtagacc ctgtcgccct tgtagtaatg cttgcgggtc
     1381 tcttcggtaa gctgtagtac ctcctcggcc acctggaacc agaggcctga cgttcggcag
     1441 aggtcgaacc cctcgcgggc cagcctgata gctggtgcga gcgcctctct cagggtcagc
     1501 cggccgtggc gctcgagcag tttctcccat ccccggaggg cgcctggaac cgccgacgac
     1561 gccccaccga ctatggagcg cacgccggcc ccgtacttga gaatcatagt ctgcggggcg
     1621 ccgcccgcac cgaatgagga ggccggaagc cccttgcctg gcatcgtgtc gtagtagtca
     1681 atcagctcgg ccccgccaga cgggtccctg acgagggcga agccgccgcc cccgatcgaa
     1741 ctcatcagcg gctcggtaac gccgacggcc gccgccgccg cgacggcagc atccgccgcg
     1801 ttgcccccgg cacggtacgt ctccgccgcc gcgtcggccg cgtaaggcgt gccagcggcg
     1861 gcggcgaatg aatgattgag gccagtacta ttcacaacca gcta
//
LOCUS       DBNGVD010000224         2902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4601317, whole genome shotgun sequence.
ACCESSION   DBNGVD010000224 DBNGVD010000000
VERSION     DBNGVD010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4601317"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="AC16CM_06245"
     CDS             complement(<1..923)
                     /locus_tag="AC16CM_06245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR3139465.1"
                     /translation="MLEDFEHRYRVVQGRDARFDGSFYVAVTSTGIYCRPSCPAVTPR
                     RANVRFYPSAAAAQGAGFRACKRCRPDAVPGSPEWDVGADVVARAMRLIADGLVDREG
                     VPGLARHLGYTERHLNRLLAAEVGAGPLAIARARRAHNARLLIETTTLPITEIAFAAG
                     FTSVRQFNGTIREVFAATPRELRRVRHRGDAGSGGISLRLPFREPFDARGMLWFLGTR
                     AVTGVEHFDGETYRRTLRLPHGAGTVALSDGGDHVLCVLELEELRDLGTAVQRCRRLL
                     DLDADPAAVTDVLGDDPMLGPIVRRSPGRRVP"
     gene            complement(1063..2589)
                     /locus_tag="AC16CM_06250"
     CDS             complement(1063..2589)
                     /locus_tag="AC16CM_06250"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3139466.1"
                     /translation="MEEREARRSPSGPAIAPGGKDQAEYTKRLRRLELESSPLDPDAE
                     RRASLRDRVVAHADSFTECMDRRTVYVASDDDGSALYDLPISEGPMDAESVLGLLDQV
                     LNRSGITVGSPGHMGYIPGSNLHAAALGDYLGAVLNKYAGLYFASPGMVRMDRVLLRW
                     VADFVGYPVSASGDLTSGGSIANLIGVVTAREACGLRARDFEKTVVYLGAQTHHAIDK
                     ALRIAGLGETVKRFVPLDDRYRMSPGALEEAIEADTKTGLKPWLIAATAGTTDTGAVD
                     PLDSIADVARRHDLWLHVDGAYGAAFALTEHGGRVLRGIGRSDSMVMDPHKGLFMPFG
                     SGIALVRDGRHLLHAHHYDASYLQDQDVLASGDEISPADLSPELTRPSRALRLWLPLK
                     LAGVAPFRAALEEKLLLARYFHERMLHEDRFEVGPAPDLSIVTFRYLPERGDANAFNR
                     KLVDAVQRDGRIFISSTTIEGRFTLRLAILSLRTHLETVDLAITILREKARQLERDHR
                     "
     gene            complement(2702..>2902)
                     /locus_tag="AC16CM_06255"
     CDS             complement(2702..>2902)
                     /locus_tag="AC16CM_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139467.1"
                     /translation="LMADNPRGIVKNVASLDKKERDQVILMHDIHPQDVRALPGVIDH
                     LERSGRQFVGVDELLRDKYPVP"
BASE COUNT          470 a          936 c         1008 g          488 t
ORIGIN      
        1 ggcacgcgcc gcccgggtga acggcggacg atcggcccga gcatcgggtc gtcgccgagc
       61 acgtcggtga cggcggcggg gtccgcatcg aggtctagta gcctgcggca gcgttgcacc
      121 gcggtcccga ggtccctgag ttcctccaac tcgagcacgc acagcacgtg gtcgccgccg
      181 tcggagagcg ccaccgtgcc ggcgccgtgc ggcaggcgca gcgtccgccg gtaggtctcc
      241 ccgtcaaagt gctcgacgcc ggttacggcc ctcgtcccga ggaaccacag catgccgcgg
      301 gcgtcgaaag gctccctgaa cggcaggcgg agggagatac caccagatcc agcgtcgccc
      361 cggtggcgaa ctcggcgcag ctccctcggg gtggccgcga agacctcgcg tatggttccg
      421 ttgaactgac gaacgctggt gaaaccagcg gcgaacgcta tttcggttat cgggagggtg
      481 gtggtctcga tgagcagcct ggcgttgtgg gcgcgtcggg cgcgggcgat ggcgagcgga
      541 ccggccccga cctcggccgc gagcagacgg ttcaggtggc gctccgtgta accgagatgc
      601 cgtgcgagac cgggaacacc ctccctgtct acaagcccgt cagcgatgag ccgcatcgcc
      661 ctggccacga cgtcggcccc gacgtcccac tccggcgagc ctggcacggc gtcgggcctg
      721 caccgcttgc atgcgcggaa cccggctccc tgcgccgccg cggcactcgg gtagaagcgt
      781 acgttcgcac gccggggcgt tacggccggg caactgggcc ggcagtagat ccccgttgag
      841 gtgacggcca cgtagaagct gccgtcgaag cgggcgtcac ggccctgcac gacacgatag
      901 cgatgttcga agtcttctag catggtcgga ttgtcgcacc caggcgatgg aattactggc
      961 gagaatcgga catcggcacg ggacgatcgg ggagatgaaa ccagggtgga gggaggctac
     1021 gggttacggg tctcaggcat cgggatctcg ctggaagggg cgtcatcgat ggtcgcgttc
     1081 gagttgcctg gctttctcgc gcaggatggt gatggcgagg tcaacggtct cgaggtgggt
     1141 gcgcaggctc aggatggcca ggcgcagggt gaagcggcct tcgatcgtgg tcgatgagat
     1201 gaagatgcgt ccatctcgtt gcacggcgtc aaccagcttg cggttgaacg cgttggcgtc
     1261 tccgcgctcc ggtaggtagc ggaacgtcac gatcgacagg tccggcgcgg gtccaacctc
     1321 gaagcggtct tcgtgcagca tccgctcgtg gaagtagcgc gcgaggagca gcttctcctc
     1381 cagcgcggcg cgaaacgggg cgacgccggc gagcttgagg gggagccaca ggcgcagcgc
     1441 cctagacggg cgcgttagct cgggcgagag atcggcgggg gagatctcgt cgccggaggc
     1501 gagcacgtcc tggtcttgca ggtagcttgc gtcgtagtgg tgggcgtgca ggagatggcg
     1561 gccgtccctg accagcgcga taccgcttcc aaacggcata aacaggccct tgtgcgggtc
     1621 catgaccatg gagtccgagc ggcctatccc ccgcagcacg cgcccgccgt gctcggtaag
     1681 ggcgaaggcc gcgccgtatg caccgtcgac gtgcagccaa agatcgtgac ggcgggcgac
     1741 gtccgcgatg ctgtcgagag gatccacggc gcccgtgtcc gtggttcctg cggtagccgc
     1801 gataagccac ggtttcaggc cggtcttcgt gtcggcctcg atcgcttctt caagcgcccc
     1861 tgggctcatg cggtaccgat cgtcgagtgg aacgaagcgc tttacggtct cgccgagccc
     1921 tgcgatccgg agcgccttgt cgatggcgtg gtgcgtctgc gcgccgaggt agacgacggt
     1981 cttctcgaag tccctcgccc tgaggccgca agcctccctg gccgtcacga ccccgatgag
     2041 gttggcgatg cttcccccgc tcgtgagatc ccccgaagcc gacaccgggt accccacgaa
     2101 atctgccacc cacctgagga gcacgcgatc catacgcacc atcccgggcg acgcgaagta
     2161 cagcccggcg tacttgttca ggacggcccc caggtagtca ccgagggccg cggcgtgcag
     2221 gttgctcccc gggatgtagc ccatgtggcc gggcgagcct acggttatgc cgctcctgtt
     2281 cagaacctgg tctagcagcc ccaggaccga ttcggcgtcc atgggtccct cagagatggg
     2341 caaatcgtac agtgcgctac cgtcgtcatc ggaggccacg tacacggtcc tgcgatccat
     2401 gcactccgtg aagctgtcgg cgtgggcaac gaccctgtcc ctcagagatg ccctacgttc
     2461 ggcgtcgggg tccaaaggac tggactctag ctcgagtcgc ctcaaccgct tcgtgtactc
     2521 agcctggtcc ttaccgcccg gtgcgatggc cggccccgag ggggagcgac gtgcctcccg
     2581 ttcctccata ggccaccttc ctcgcctcgc gtacccccag gaggatatac gaagttgcca
     2641 gaagctgtcg cgaagatccc cacggcgttg ccgcgcgggc ggggccgtta tccgggaagc
     2701 tctacggtac ggggtacttg tcgcgcagca gctcgtccac gccgacgaac tggcggcccg
     2761 agcgctcgag gtggtcgatg acgccgggca gagcccgcac gtcctgggga tggatgtcgt
     2821 gcatgaggat cacctggtcg cgttccttct tgtccaggct ggcgacgttc tttactatgc
     2881 cccgcgggtt gtcggccata ag
//
LOCUS       DBNGVD010000225         5243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4966340, whole genome shotgun sequence.
ACCESSION   DBNGVD010000225 DBNGVD010000000
VERSION     DBNGVD010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4966340"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="AC16CM_06260"
     CDS             complement(<1..580)
                     /locus_tag="AC16CM_06260"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HIR3139468.1"
                     /translation="MEINRSLKLAIDVLLGSVVPILVLSYLSAPMGAVPAYLLSALIP
                     VGWVMADLFFLSRRFNFITAFLGLNAIVRGLLAFWFVDGALYAFKDTVGSILIVVVFG
                     ASLLWGRPLLGAFVAQALDPRTPEQEASLERLFAERPVARTLLISTAGLALLNTATAT
                     INIVLNLRIVTAPFGTGEFNSQVAQVNALTRLT"
     gene            complement(985..1097)
                     /locus_tag="AC16CM_06265"
                     /pseudo
     CDS             complement(985..1097)
                     /locus_tag="AC16CM_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907679.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1353..1628
                     /locus_tag="AC16CM_06270"
     CDS             1353..1628
                     /locus_tag="AC16CM_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3139469.1"
                     /translation="MREAILDTTGALVAEHGLLSATMSRVAEETGIGRVALYKYFPDV
                     ESILLAWHERDVGGHLERLSLRPTHSSSTRATPANSRRSRIGASMPT"
     gene            1904..2143
                     /locus_tag="AC16CM_06275"
     CDS             1904..2143
                     /locus_tag="AC16CM_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139470.1"
                     /translation="MSDGLKVLLGVLVGAIVVLLFVATLGGGTMMGGMGSMMGGSMMG
                     GGIFGILFAVLFWAVLVALLIALVLWIVNQTQQRR"
     gene            2188..3309
                     /locus_tag="AC16CM_06280"
     CDS             2188..3309
                     /locus_tag="AC16CM_06280"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3139471.1"
                     /translation="MPNGTDILITGGYGTVGGRVAADLAPDYPDRVVVAGRSAEKATR
                     LAGELGHGARARRVDVGDPDSVEAALGGVGLVVSCIDQPEPHLLRAAIARGLAYTDIA
                     PHLMTRRPTEAMKAEATRTGARIVLGSGLAPGISSLLARLGADRVGVVESVESNVLLS
                     VGDAYGPASRAYLLEEISLSYAVRFKGRETSARSFGGHARVTFPPPFGRRTAYLFPFS
                     DQVFFPETLGARTALSRLALEPPWLGALLSALVRLRVTAMLGRRAGAKERVQRLNAWL
                     RCRHEGHDWYGVAVEVEGARGRVRASLLGRGQATGTAVGAAAVVRALARDEVRQAGIW
                     LAEEVVAPGSFFEHLAARGLVPTVEEIPEGKRTVRHSKG"
     gene            3575..4279
                     /locus_tag="AC16CM_06285"
     CDS             3575..4279
                     /locus_tag="AC16CM_06285"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3139472.1"
                     /translation="MATEDEKGKIIAVVAYPGVALLDLVATQTVLDRGTKYRTVLVGE
                     RTEPLGSDTPLAIVPEKGFEEVPAPYAIVVPGGGMASLEAMGNERLLNYLRFAEHSAE
                     LIVSVSTGAFVLAAAGLLEGRRATTHLAYSELLEKLGVDYVRGYSVEDGKFLSMEGVS
                     SAIDTTLGLVAKLRGEAAAKRAEMIIEYDPQPPFGPIDWGGANGEGLGDELDEHRADL
                     ERALSNRPDLYRKLLG"
     gene            4337..5038
                     /locus_tag="AC16CM_06290"
     CDS             4337..5038
                     /locus_tag="AC16CM_06290"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3139473.1"
                     /translation="MATQEQKTIAFVVYPGLTLFDLAGPLQIFAGVSRLAPEYRAVIV
                     SERSEPLDTDLRVKMVPTHAFEEVPNPHVLLVPGDRSSTFTAMRNESLRSYVRSAAET
                     AEVAGSICTGALVVASVGLLEGRRATTHWVYYKILEQFGATYVRRRWVEDGKFISSAG
                     VTAGIDMALHLAARLTDEETARRVQRSLGYDPRPPYGGMDYEHLGAMSAVRAGLSLAA
                     PVIAARPKRMIGQNR"
BASE COUNT          934 a         1672 c         1757 g          880 t
ORIGIN      
        1 gcgtcagccg ggtcagggcg ttcacctgcg ccacctggga gttgaactcc cccgtgccga
       61 agggggcggt cactatccgc aggttaagca cgatattgat cgtggcggtg gcggtgttca
      121 gcaacgccag ccccgcggtg ctgatcagaa gcgttcgagc tacggggcgt tcggcgaaga
      181 ggcgctccag cgaagcctcc tgctcggggg tgcggggatc gagggcctgg gctacgaagg
      241 ctccaagcag ggggcgaccc cacagcagcg aggcgccaaa gaccacgacg atcaggatgc
      301 tgccgacggt gtctttgaag gcgtagagcg ccccgtcgac gaaccagaag gccagcagac
      361 cgcgaacgat ggcgttgagg cccagaaagg cggtaatgaa gttgaagcgc ctgctgagaa
      421 aaaacaggtc ggccatcacc cagcccacgg ggatgagggc ggagagcagg taggcgggta
      481 cagcccccat cggagccgaa aggtaggaga ggaccaggat cgggacgacc gagcccagca
      541 gaacgtcgat ggcgagtttg agtgagcggt tgatttccac tcctaccgcc ttcccgaccg
      601 ctgccttttg catgtccttt gacctccgtc tctctcgcgc tctacaaggc atcttcagta
      661 aagcccacgg ctcgggcgcc tccctcggcc gatggactac tttcgcgcta ggactttggc
      721 cctacctcag accaccatcg ctcgacctag cccccaccac gaaacgatgt ctctgggagc
      781 tccacgcacc ctccccaagg cggagctcga gcaacgccgc gcgatggcgg gggtaggcac
      841 gagggtgtag gcgatcctta ccggaggcca agagcactcc ccatccccag ttgtgtggcc
      901 gagtcctagc ggtgcacggg gctgcccctg gtaccaagga aattcgaact tctcagaacg
      961 tcgttaggaa actgtctgaa aacctcacga gcgggccaat ctcctcgcca taaggcgact
     1021 catagccacg tagatgaacg cttctgcgct ctctggcagc ctctcgtaac ccataggctc
     1081 atcctcctgt tctgagatag ccacgagaga gaaggtcctc tccacctagg gctcctcggc
     1141 aggaacggcc tcggaccttg ctccggcatg aacttcttaa cgtcgaccgg gaccccctcc
     1201 ttattgagcc ccctcaccca tgcccacatc acctcttcgg gagcgagctt tctggggtgc
     1261 ctgacgatct gcgcactcca ccccagggtc ttctccaccc agcctatgcc cctgtgctcc
     1321 tgtcccggtg tatccggcct ccagccaaag aggtgcgaga ggcgatcctg gacacgaccg
     1381 gggcgctggt ggccgagcac gggctgcttt cggcgacgat gtcgcgcgtt gccgaggaaa
     1441 ccggcatcgg gcgtgtggcg ctctacaagt atttcccgga cgtcgaatcg atcctgctcg
     1501 cctggcacga acgtgacgtc ggcggccacc tcgaacggct cagcttgagg cccacgcatt
     1561 catcgagcac aagggccacg ccagcgaact ctcggcgctc ccggatcggg gcgagcatgc
     1621 cgacctagcg cggcgacacc tcgcggacat catcctggac cgtactgtct ttccgaactc
     1681 gcggcggcca gcggccttcc gaacgaggcc gcgatccgcc gactcgtaac ggtcaccctg
     1741 gacgggttgc gtcccccgcg ctgaccgagc gctcggacgc cctttttgcc ttaggagctc
     1801 gcccgattgg cgaaagctgc ccaggcaaac gaacaatctt gctcatgcgc cacagatgct
     1861 gggtgcgaca ctgggttcag gagtaaagaa ggaaggtgcg gccatgagcg acggtttgaa
     1921 ggtcctcctc ggggtattgg tcggcgcgat agtcgtcctg ttgttcgttg cgaccctcgg
     1981 tgggggcacc atgatgggcg gcatgggctc catgatgggc ggttccatga tgggcggtgg
     2041 catcttcggc atcctgttcg ccgtgctgtt ctgggccgtg cttgtagcct tgctcatagc
     2101 gttggttctc tggatcgtca accagaccca gcagcgccgt tagcccctgg gaaccgactc
     2161 ttcgcgcggg tcggaaggta acatcccatg ccgaatggta ccgacatcct gatcaccggc
     2221 ggctacggca ccgtgggagg ccgcgtggcg gcggatctcg cgccagacta tccggatcgc
     2281 gtcgtcgtgg cgggtcggag cgccgagaag gcaactcggc tcgcgggcga gcttgggcac
     2341 ggcgcgcgag cccgcagggt cgacgttggc gaccccgatt cggtggaggc cgctctgggc
     2401 ggcgtcggcc tggtggtgag ctgtatcgac cagcccgagc cacacctgtt gcgcgcggcc
     2461 atcgcgcgcg gcctggccta cacggacata gcgccccacc tcatgacccg ccgtcccacc
     2521 gaagccatga aggccgaggc gacacggacg ggcgcccgaa tcgtactggg gagcgggctc
     2581 gcgcccggca tctcgagcct gctcgcccgc ctgggggcgg acagggtcgg cgtcgtcgag
     2641 agcgtagaga gcaatgtgtt actctccgtc ggcgacgcct atgggcccgc gtcgcgcgca
     2701 tatcttctgg aggagatctc gctgtcctac gcggtgcgtt tcaagggccg cgagacgtcc
     2761 gcgcggtcct tcggcgggca cgcccgcgtc acattcccgc ctcctttcgg taggaggacg
     2821 gcctaccttt tcccgttctc cgaccaggtc ttcttccccg agaccctcgg tgcccgtacg
     2881 gcgctctccc gcctggcgct cgaaccgccg tggcttggag cgctgttgtc ggcgctcgta
     2941 cggctaaggg tgacggcgat gctcgggcgt cgggcgggcg ccaaggaacg agtccaacgc
     3001 ctcaacgcgt ggctcaggtg ccggcacgag gggcacgact ggtacggtgt cgctgtggag
     3061 gttgagggag cgcgcggccg ggttcgggct tctctgctgg gccgcggcca ggctaccggg
     3121 acggccgtag gagcggcagc tgtggtgcgt gcgctcgcta gagatgaggt taggcaggcg
     3181 gggatctggc ttgcggagga ggtcgtggcg ccggggtctt tcttcgaaca cctggcggcc
     3241 cgcggcctgg tgccgacggt tgaggaaata cccgaaggca agcgcaccgt tcggcacagc
     3301 aagggctgaa gctcctcggt cggcccatga tcgcgcctcg gaatcgccag cgccactaga
     3361 ggacaacact aggggacaag agtaggagga gctcgatccg cggtgaaggt ggagcgggtt
     3421 cggtcccggc gccccgcgcg ggaggccggc ggccaccctc aagtgccttc tattattatc
     3481 gccatacatg taaactgacg cgagggaaag ctggaggata cgaaaggcct agttagacat
     3541 ttatcgtacc tcccgacaag gagtcgaggt agatttggca acggaagacg agaagggcaa
     3601 gattattgcc gtcgtcgcct acccgggggt ggcgctgctc gacctcgtcg cgacgcagac
     3661 cgtgctcgac aggggtacca agtaccggac ggtcctcgta ggcgagcgca cagagccctt
     3721 gggttccgat acccctctgg cgatcgttcc cgagaagggg ttcgaggagg tgcccgcccc
     3781 ttatgctatc gtcgtccccg gcgggggcat ggcctcccta gaggccatgg gaaacgagag
     3841 gctcctaaac tacctgcgct tcgcggagca ctcggccgag ctgatcgtat cggtgtccac
     3901 gggcgccttc gtcctggccg cggccggcct gctcgaaggt cggcgcgcga ccacccacct
     3961 agcctactcg gagctgctgg agaagctcgg ggtggactac gtccggggct actcggtcga
     4021 ggacggcaag ttcctgagca tggaaggggt ctcgagcgcg atcgacacta cgctgggatt
     4081 ggtggccaaa ctcagaggcg aagccgcggc caagcgcgcg gagatgatca tcgagtacga
     4141 cccccagccg cccttcgggc cgatagactg gggcggggcg aacggagaag ggctagggga
     4201 cgagctggac gagcacaggg cagacctgga gcgggcgctt tcgaatcggc cggatctcta
     4261 ccggaagttg ctcggctgaa gggcaccact cggcttcgtc cctacacacc ggacaggaaa
     4321 ggaaataaga atacgaatgg cgacacaaga acagaagacc atcgctttcg tcgtctaccc
     4381 gggactgacg ctcttcgacc tggccggacc gctccagatc ttcgcgggcg tgagccggct
     4441 cgcccccgaa tacagggcgg tgatcgtaag cgagcgctcg gaacccttgg acaccgattt
     4501 gcgcgtgaag atggtcccga cccacgcctt cgaggaggta cccaaccctc acgtcctgct
     4561 tgtccccggc gaccgctcct cgacgtttac ggccatgaga aacgagtcgc tgcggagcta
     4621 cgtgcgttcg gcggccgaaa ccgcagaggt ggcagggtcg atctgcaccg gggcgctcgt
     4681 cgtggcgtcc gtgggattgc tggagggcag gcgggcgacc acccactggg tctactacaa
     4741 gatcctggag cagttcgggg ccacctacgt tcgcaggaga tgggtagaag acggcaagtt
     4801 catctcctca gccggcgtca ccgccgggat cgacatggcg ctgcacctgg cggcgaggct
     4861 caccgacgag gagacagcca ggcgggtgca gcgctcgctg ggctacgacc cgcgcccgcc
     4921 gtacgggggc atggactacg agcacctcgg cgcgatgtcc gccgtgaggg ccggccttag
     4981 cctggcggcc cccgtcatcg cggccagacc gaagcgcatg atcgggcaaa accgctgaag
     5041 cggatcgagt catggctcgg tggggcggtc ctcgagcagg ccaagtgacg accgcccacc
     5101 gcgccgggac cacgaaagga gaggttgtgg agcaccagga cgcttttccg accgagatca
     5161 gggggctccc gcaggctgcg agcccgagca cggctcgcct ccgcgacggc gattttgtcg
     5221 aactgcgcat ccgccccgtg cgc
//
LOCUS       DBNGVD010000226        13685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4783574, whole genome shotgun sequence.
ACCESSION   DBNGVD010000226 DBNGVD010000000
VERSION     DBNGVD010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4783574"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="sigJ"
                     /locus_tag="AC16CM_06295"
     CDS             complement(<1..778)
                     /gene="sigJ"
                     /locus_tag="AC16CM_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HIR3139474.1"
                     /translation="MSDDPFVRHRSLLFTVAYEMLGSAADAEDVVQETWLRWADLGTD
                     QADVRNPRAYLVRIVTRQALNRMRTLARRREEYVGEWLPEPLPTGPDVAEDAELAESV
                     SIAMLTVLESLAPAERAVFVLREVFDVPYAEIAAAVDKTPAAVRQIAHRARGHVAARR
                     PRVRVDRAEHQRVVERFLAAIGSGDLQVLLDVLAPEVVLVADGGGEVAAIRRPVEGRD
                     LVATLLSRFRTLSPDAVVGTAWLNGAPAVRIDLAGEPDTAV"
     gene            complement(775..1368)
                     /locus_tag="AC16CM_06300"
     CDS             complement(775..1368)
                     /locus_tag="AC16CM_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3139475.1"
                     /translation="MDGNTRIPKAELTGLYGAVVKRMSRKMMGDVPEPVEVAWHNRKV
                     LNFSFNIGRQAQKWDQCDENLKSFAHMAVASLIGCSFCLDFGYFQAHNDGLDLTKARE
                     VPRWRESVIFTPLERDVMEYAEAMSQTPPTVTDEMSARLLGQLGAPALVELTAFIGLA
                     NLYTRTNTAFGIESQGFSASCQLAPLTTVGNGVRSTA"
     gene            1671..2834
                     /locus_tag="AC16CM_06305"
     CDS             1671..2834
                     /locus_tag="AC16CM_06305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3139476.1"
                     /translation="MREPVILEAVRTPFGKAGGAFRGVRPDALLARALDGLLERSGLK
                     AEKVEDVINGTVTQAGEQGANVGRLGVLLAGFPFTVPAVSLNRMCGSSQQAAHFASQA
                     IAAGDVGYAIASGVESMTRVPMFSDTGDGVSGFEVNPDLLEKHNLVHQGESAERIAER
                     WKISRRDLDAFATESHRRASAAAHEGKNVEVLPSLGLDADGNEVELTADEGIRNNIDQ
                     EKMAGLKTVFRPEGDGVVTAGNSSQLTDGASAVLIADRERALADGFTPRARFLARIAV
                     GEDPVMQLTGVIPATRLALEKAGLGMDDLDWIEINEAFASVVLSWADELAPDMERVNP
                     WGGAIAHGHPLGATGAGLMAKMIAGLEATDGQFGLQVMCIGHGMSTATIVERI"
     gene            2952..5351
                     /locus_tag="AC16CM_06310"
     CDS             2952..5351
                     /locus_tag="AC16CM_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3139477.1"
                     /translation="MVIQPSEPGKYSRRTRGDLWESEERFRLLVEGVEEYAIFMIDSG
                     GYVSTWNPGARRIKGYEAGEIVGEHFSVFFTGEDVERGHPEELLRLAASEGRYEEEGL
                     RVRKDGSTFWANAVVTALRNESGDLRGFAKVTRDITARKKAEERERLLLREQAARERA
                     TDILESISDAFYAVDTRWRFTYVNGRAEELLGRSREELLGKNVWEEFPEAVNRQAYHQ
                     ARRAMEEGVTTEFEAELAGGAWVSGRVYPSREGLSVYFRDETENHRNEEAQRFLVQAS
                     AVLSSSLDYNETLASVARLAVPTLADWCTVHFVEEDGVSRRLALEHTDPEKLRLAHEL
                     EQRYPPDPDAPQGLYNVLRTREPELVARIPDELLEQVARDDRHRELLIGLGLRSYMVV
                     PLLARGRVLGAISFISAESERRYGEADLRLAEELARRAAYAVDNARLYKEAQKEIAER
                     RRAQEELRASRDQLEAILLGAAEGITAQDPTGKIIYANEAAARMTGFSSVQEMTTSSP
                     DEVLARYEIRDEEGNLLPIEKLPGRRALLGEEGVEEIICFRILSTEEVRWATMKAMPI
                     FGERGTVLMAVSILRDITESRRAKEALRRVTEAERSRIARDLHDGVLQDLSYSAATLG
                     MLMLQPGDEKLKEQLQMAIDAVRSAAQGLREVVNGLRLEGEEGRPFTATIEALIRRNR
                     TMARKATINLHIDERVPEASLGETGTQISHVIQEALTNARRHSGAEVISVSLRMNGDV
                     LVAEVSDNGQGFGEGTRPGVGLESMRERAALIGGELEIKSESEQGTSVRLEAPLPGKS
                     S"
     gene            5387..5743
                     /locus_tag="AC16CM_06315"
     CDS             5387..5743
                     /locus_tag="AC16CM_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139478.1"
                     /translation="MHVEDHAGFRDLMKILLDGQSDMEVVAQAGSLHEARVQAAGSEV
                     DVAVLDLSLPDGNGLDLIPELRRANPSVGVIVLSANLDPAGPERASGAGADEILDKLA
                     PVEAVLNSVRKLGIRD"
     gene            complement(5963..6385)
                     /locus_tag="AC16CM_06320"
     CDS             complement(5963..6385)
                     /locus_tag="AC16CM_06320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3139479.1"
                     /translation="MRQIAAVLLFCAMFSAVLLAGPTTKAHAQASGQAVVDTASSYIG
                     TPYGAYGLDCSGFTSAVFADLGVSIPDSPGGQYAYGVPSNAKAGDLVFFDEGGYGISH
                     VGIATGYGTVIHSSTYFGEVVESDMSYISGYVGAVDAY"
     gene            complement(7023..7334)
                     /locus_tag="AC16CM_06325"
     CDS             complement(7023..7334)
                     /locus_tag="AC16CM_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3139480.1"
                     /translation="MREIDKSYAPQVDRPGIGHLLERARISRGVSLDEAERATLIRKD
                     YLQRLESDDHSAMPEPIYVRGFVKAYADYLGLDGDRFAAQIALWQERRRRTRRGPLWD
                     G"
     gene            complement(7336..7707)
                     /locus_tag="AC16CM_06330"
     CDS             complement(7336..7707)
                     /locus_tag="AC16CM_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139481.1"
                     /translation="MRVLMANEPRSHQEVFAGALRVLRPHVEVITVRPDALEGEAPGL
                     RPDAVVCSRVTPALRTAAGRWMEVRLEEGALLVRTSDAGNSPDPDPGLDVLLQFVDRG
                     EERPEPPGNRTTPVTGKGSEV"
     gene            complement(7710..8924)
                     /locus_tag="AC16CM_06335"
     CDS             complement(7710..8924)
                     /locus_tag="AC16CM_06335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3139482.1"
                     /translation="MNAWQKRNRPSHDDKGPEQRARSSNLTEGVANLGPSHNISPALS
                     PHLDFRLKTLGGLLYVVGAIALLLFVHGGWFELPAGNTFAIDRVVLAGGILVWSVWFF
                     PWHRFGRNVLLIPVSAAIVLITLAVYFSGGWQSPLSIFYLFVVVFCAGYFSTGVAALC
                     VAVTLLASLTPQLYAPDTSSLIGYLAIQTPTYAGLVFACRYGLRERTRTQSEQASARI
                     RDLEERLQHEASLDPLTGLYNRGHFETRFREEFERARRTGERFMVLFVDVDDFKLIND
                     THGHRAGDEALQLVADVLRACSGHVNVVARHGGDEFLVMLPGASLPEARRFFEEMREM
                     VAERSGESLGLGLRLSAGAVQCPGDATDPATLLDAADEAMYRAKRRGKNRLFTGLSLA
                     PTGHRRASSRGE"
     gene            complement(9003..9416)
                     /locus_tag="AC16CM_06340"
     CDS             complement(9003..9416)
                     /locus_tag="AC16CM_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139483.1"
                     /translation="MRQPEEYRVRAASEAAFGSTEYFFGYLWDEGSYVVQQGQDGLVP
                     MRDGARRFADEAPHGGAITQRAPPLPLPSPFSGFGLTMSGAVLGASSYAEGHVPLLAV
                     IFCCLSALLWRGRYRAYGALLRAGTVPRPALERPG"
     gene            10907..11926
                     /locus_tag="AC16CM_06345"
     CDS             10907..11926
                     /locus_tag="AC16CM_06345"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3139484.1"
                     /translation="MRGLHVYSDPASGRHVNELAGVESPERFPAAIAGVEEAHRGGVP
                     VEWRSCAPAGEDDLAAVHDAAYLDGVREMARAGGGYLSPDTALGKGSWEAASLASGAA
                     CSAVDSALAGETAFAVARPPGHHAGRDYGMGFCLTNHAAVAAGHALAGGMERVAILDW
                     DVHHGNGTQGIFYDDDRVLYLSMHQSPFYPGTGEAGEVGEGKGRGFTVNVPLPAGSGE
                     DVYAAVFTGVFAPLIGEFSPGLILVSAGFDAQADDLLGGMALSSDAFARFAAFVTQLS
                     REIGAAPPAFVLEGGYNLRALTESVAACIRGATDEAPDWRYDGGVRQVEKSREVLAPF
                     WRGVG"
     gene            11933..12595
                     /locus_tag="AC16CM_06350"
     CDS             11933..12595
                     /locus_tag="AC16CM_06350"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3139485.1"
                     /translation="MRLFRSPPFEDRRDAGRKLAAKLSRFRGAGCVVFGLPRGGVPVA
                     YEISRSLGAPLDVFVSRKLGAPGQPEFGIGAVAAGGVRILNTDVIKRLGITDEYVDRI
                     TAQEAAEVNRRLRYFRGGRPEPEVAGRTAILVDDGLATGVTARAALEALKLRGPERLI
                     LAAPVCAAQTVGLFASLVDELVCLTSPSDLGAIGLWYRNFDQTTDQEVVQLLREAREE
                     LG"
     gene            12740..13399
                     /locus_tag="AC16CM_06355"
     CDS             12740..13399
                     /locus_tag="AC16CM_06355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3139486.1"
                     /translation="MCGRYTLSTPAGRLAEEFQLDSTVEIPPSYNVAPTQQVAAVLED
                     EGGRRLEMLRWGLVPSWADDPDIGSRMINARSETAPEKPSFRRAFRGRRCLIPADGFY
                     EWKRENGGKQPYYFHMQDGHPFAFAGLWESWDKGDGTLRTCTILTTRANPVLQGIHDR
                     MPVILPHDAYNAWLDPDADREELGELMIPYPEGDLEAYPVSRFVNSPRNNDERCIEPA
                     A"
     gene            13396..>13685
                     /locus_tag="AC16CM_06360"
     CDS             13396..>13685
                     /locus_tag="AC16CM_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR3139487.1"
                     /translation="MSLEVGAEVREALQGGEPVVALESTLISHGLPYPQNLEVALEAE
                     GIVRAEGAVPATIGVVGGIARIGLDGAELELMATAEGIPKLSARDLPVALAKG"
BASE COUNT         2423 a         4416 c         4639 g         2207 t
ORIGIN      
        1 tgaccgccgt atctggttcg ccggcaagat ctatgcgtac ggctggcgcg ccgttcagcc
       61 acgcggtgcc aaccaccgca tccggcgaga gcgtcctgaa gcgggagagc agggtcgcca
      121 ccaggtcacg gccctccacc ggacgccgga tggcggccac ctcgccgccg ccgtcggcga
      181 ccagcaccac ctcgggcgcc agcacgtcga gcaggacctg cagatctccg ctcccgattg
      241 ccgccaggaa tcgttccacc accctctgat gctcggcacg atctaccctc acccgcggtc
      301 tacgggcggc cacgtgtcct ctggcgcggt gggcgatctg ccggaccgcc gcgggcgtct
      361 tgtcgacagc ggcggcgatc tcggcgtagg gcacgtcgaa gacctcgcgc agcacgaaga
      421 cggcccgctc ggcgggtgca agggactcga gtaccgtcag catcgcgatc gagacgctct
      481 cggccagctc ggcgtcctcg gccacgtccg gaccggtggg cagcggctcg ggcagccact
      541 cccccacgta ctcctcgcgc ctgcgcgcca gcgtgcgcat ccggtttagc gcctgccggg
      601 tgacgatccg gaccaaatag gcccgcgggt tgcgtacgtc cgcctggtcg gtgccgaggt
      661 ccgcccagcg tagccaggtc tcctgcacga cgtcctcggc gtccgcggcc gagccgagca
      721 tctcgtaggc cacggtgaag agcaggctgc gatgacggac gaacgggtcg tcgctcatgc
      781 cgtcgagcgt accccgttgc ccactgtggt gagcggcgcg agctggcagg aggcggagaa
      841 gccctgcgac tcgatgccga acgcggtgtt ggtgcgggtg tacaggttcg ccaacccgat
      901 gaacgccgtc agctccacca gcgccggagc cccgagctgc ccgagcagcc gcgcggacat
      961 ctcgtcggtc accgtgggcg gggtctgact catcgcctca gcatactcca tgacgtcgcg
     1021 ctccagcggt gtgaagatta cggactctcg ccagcgcggc acctcgcgcg cctttgtcag
     1081 gtcgagccca tcgttgtggg cctggaagta cccgaagtcc aggcagaaac tgcacccgat
     1141 caggctggcc accgccatgt gggcgaagga cttcaggttc tcgtcgcact ggtcccactt
     1201 ctgcgcctga cgaccgatgt tgaaggagaa gttgagcacc ttcctgttgt gccacgccac
     1261 ctcgaccggc tcgggcacgt cgcccatcat cttgcgggac atccgcttga ccaccgcgcc
     1321 gtacaagccg gtcagttcgg ccttcgggat cctggtgttg ccgtccatgt tgcctccagt
     1381 gtctctctag gtttccacat aaagacaccg gcagggctcc ggttgtgaca tcttcggttg
     1441 atgttctcgg tccaggtctc aggccccgcc gctcgaaacg cgtcgagcat cgcctacccg
     1501 aagtccgagg ggaacgtgaa gttctttctc cacccctacg gcggcgctgg tggcacccgt
     1561 gagcggggaa caatacactg tgatggaacc ttttgcgcga gctcgggcac acgatcgggg
     1621 ggcactgctg cccgggagca ccaggcatct acgaggggag agaatcagaa atgagggagc
     1681 ccgtaatact ggaggccgtg cgcaccccgt tcggaaaggc cggtggtgcc ttccgcgggg
     1741 tgcgccccga cgcgctcctg gcgcgcgccc tcgacgggct actagagcgt tcggggctga
     1801 aggcggagaa ggtggaggac gtcataaacg gcaccgtcac ccaggccgga gagcaggggg
     1861 ccaacgtcgg taggctcggc gtcttgctcg cggggttccc cttcacggtg ccggcggtaa
     1921 gcctcaatcg catgtgcggc tccagccagc aggccgccca cttcgcctcg caggccatcg
     1981 ccgccgggga cgtcggctac gctatagcgt cgggcgtgga gagcatgacg cgggtgccca
     2041 tgttcagcga tacgggggac ggcgtttcgg ggttcgaggt gaacccggat ctcctggaga
     2101 aacacaacct cgtgcatcag ggcgagagcg ccgagcggat cgcggaacgg tggaagatct
     2161 cgcgccggga cctagacgct ttcgccaccg agagccaccg gcgggcgagc gcggcggccc
     2221 acgagggaaa gaacgtcgag gtattgccct cccttggcct ggacgccgac ggcaacgagg
     2281 tagagctcac ggcggatgag ggcatccgta acaacataga ccaggagaag atggccgggc
     2341 tcaagaccgt cttcaggccg gagggagatg gcgtggtgac ggcgggcaac tcctcccagc
     2401 tcaccgacgg ggcctcagcg gtgctcatcg cggaccggga gagggcgttg gccgacggct
     2461 tcacgccgag ggcgcgcttc ctggcgcgga tcgcagtggg tgaggacccc gtcatgcaac
     2521 tcaccggcgt catcccggcc acgcgcctgg cgctagagaa ggcggggctc ggcatggatg
     2581 acctggactg gatcgagata aacgaggcct tcgcctcggt cgtcctctcc tgggccgacg
     2641 agctagcccc cgacatggag cgggtaaacc cctggggtgg ggccatagca cacggccacc
     2701 cgttgggggc caccggggcc ggcctgatgg cgaagatgat agccggcctg gaagccacag
     2761 atggccaatt cggcctgcag gtcatgtgca tcggccatgg catgtccacc gccacgatcg
     2821 tggagcgcat ctagtcaggg atctcggcta catcgaccgt aggattctcc atgatgcacg
     2881 tcgctccaac tcgaaactcc ggaccccctt gtgagaaaat acctatcttc ggccttggag
     2941 gtgtagaagt agtggtgatt caaccgtccg aacctggcaa gtactcgagg cggaccaggg
     3001 gcgatctgtg ggagagcgag gagcgcttcc ggctgctggt tgaaggggta gaagaatacg
     3061 ccatcttcat gatcgactca ggtggatatg tctccacctg gaacccggga gcccggcgta
     3121 tcaagggtta cgaggcgggg gagatcgttg gcgagcattt ttcggttttc tttaccgggg
     3181 aggacgtgga gcgcggacac cccgaagagc tgctgcgcct cgccgcctca gaggggcgat
     3241 acgaagaaga gggcctgagg gtccgtaagg acggatcgac cttctgggcg aacgcggtcg
     3301 taaccgcgct caggaacgaa tcgggagacc tgcgtgggtt cgccaaggtg accagagaca
     3361 taacggcgcg caagaaggcc gaagaacgtg aacgcctgtt gctccgcgag caggcggcca
     3421 gggagcgtgc caccgacata ctcgagagca tcagtgacgc cttctacgcc gtagacacgc
     3481 ggtggcgctt tacctacgtc aacggcaggg ccgaggagct cctgggccgc tcccgggaag
     3541 agctgctggg caagaacgtc tgggaggagt tcccagaggc ggtaaaccgc caggcgtacc
     3601 accaggcccg gcgcgccatg gaagaaggcg tcaccacgga gttcgaggcc gagctcgcgg
     3661 gcggcgcgtg ggtgtcggga cgggtctatc cctcacggga aggtctctcg gtgtacttca
     3721 gggatgagac cgagaaccac aggaacgagg aggcgcagcg ctttctcgtc caggccagcg
     3781 ccgtactctc ctcctccctt gactacaacg agacccttgc cagcgtggcg cgcctcgcgg
     3841 tacccacgct cgccgactgg tgtaccgtgc acttcgtcga ggaagacggc gtttccagga
     3901 ggctcgccct cgaacacacc gacccggaga agttgcggct ggcccacgag ctagaacaac
     3961 ggtacccgcc cgatcccgac gccccgcagg ggctatacaa cgtgctgagg acccgcgagc
     4021 ccgagctggt ggccaggata cctgatgagc tcctggagca ggtggcccgc gacgacaggc
     4081 acagggaatt gctgatcgga ttgggcctac gctcatacat ggtggtaccc ctccttgctc
     4141 ggggccgcgt cctcggggcg ataagcttca tctcggccga gtcggaacgg cgctacggtg
     4201 aggccgacct gcggctggcg gaggaactgg cccgtcgcgc cgcctacgcg gtcgacaacg
     4261 ccaggctcta caaggaagcg cagaaggaga tcgccgaacg ccgaagggcg caggaggagt
     4321 tgagggcgtc gagggaccag ctcgaggcca tcctgctggg ggccgccgag ggcataaccg
     4381 cccaggaccc aacggggaag atcatctacg cgaacgaggc ggcggcccgg atgaccggct
     4441 tctcctccgt gcaggagatg acgacctcat cgccagacga ggtcctggcc aggtacgaga
     4501 tccgggacga agagggcaat ctgctcccca tcgagaagct tcccgggcgc agagccctcc
     4561 ttggtgagga aggggtcgag gagataatat gcttccgcat cctctccacg gaagaagtgc
     4621 ggtgggccac gatgaaggcc atgcccatct tcggggagcg gggaacggtc ctcatggcgg
     4681 tcagcatcct gcgcgatatt accgagagca ggcgggccaa ggaggccctc cggcgtgtga
     4741 ccgaggccga gcgcagcagg atagcgcgcg acctgcacga tggtgttctg caggatctct
     4801 cctactcggc ggccaccctc ggcatgctca tgctccaacc cggggatgag aagctgaagg
     4861 agcagctaca gatggccatc gacgccgtca gaagcgccgc ccagggcctg cgcgaggtgg
     4921 tcaatggcct gcgcctcgaa ggcgaggaag gcaggccctt cacggcgacg attgaggccc
     4981 tgatcaggcg caaccgcacc atggccagga aagccacgat caacctgcat atcgacgaac
     5041 gggtcccgga ggcctcgctc ggggagaccg ggacccagat ctcccacgtg atccaggagg
     5101 ccctcacgaa cgcgcgcagg cattcggggg ctgaggtgat ctccgtgagc cttcgtatga
     5161 acggggatgt cctggtcgcc gaggtctccg ataacggcca gggctttgga gaaggcacga
     5221 gacccggcgt cggcctggaa agcatgcgtg aaagggccgc cctgatcggc ggcgaactgg
     5281 agatcaagag cgaatcggaa cagggcacga gcgtgcgttt agaagctccc ttaccgggaa
     5341 agtcttcttg atgggcgaag ggatagatac cagggaaatc cgtgtgatgc acgtagagga
     5401 ccatgccggc ttcagggatc tgatgaagat cctgctagac ggccagtcgg acatggaggt
     5461 ggtggcgcag gccgggtccc tgcacgaggc acgtgtccag gccgcaggct ccgaagtcga
     5521 tgtggcggtc ctcgatttga gcctcccgga cggaaacggt ctggacctga tccccgagct
     5581 tcgccgggct aacccgagcg tgggtgtgat cgtgctgagc gccaacctcg accctgctgg
     5641 cccggagaga gcctccggcg cgggcgccga cgagatcctg gacaaactag cccctgtaga
     5701 ggcggtcctg aactccgtta gaaaactggg gatcagggac taaatagtac tcgtcgctat
     5761 ccccacagca ccacaaccag gcagatctga aaagacaaac ggtaaaccag gaggccgggg
     5821 ccggcgcttg gtagccgacc ccgacctcct ggttcctgga acagttagcg gacgaactga
     5881 tcctcaacga tagcaatcag ttgtcggtcc tcgggatcag cactccttgc ctgatagctg
     5941 aggcctaact gctagctttc atctagtacg cgtcgacggc gcccacgtaa ccagagatgt
     6001 agctcatgtc ggactccacg acttcaccga agtaggtgga agagtggatc acggtcccgt
     6061 agccggtggc tatgccgacg tgcgagatgc cgtaaccgcc ctcatcgaag aacaccaggt
     6121 cacctgcctt ggcgttcgag ggcacgccat aagcgtactg gccacctggc gagtcgggta
     6181 tggacacgcc gaggtcagcg aacaccgccg aggtgaagcc ggagcaatcc agaccgtagg
     6241 caccataggg cgtcccgatg taggagctcg cggtgtcgac gacggcctgc cccgaggcct
     6301 gggcgtgcgc ctttgtggtc ggacccgcca gtaggacggc cgagaacatg gcgcagaata
     6361 gcaggacagc agcaatctgc cgcaaaatga atccccctcc agttttttct tgcacaacga
     6421 cggcggatgc taacagagac gattctgatg tcaagtaggg tgcaactcgt cctcgagagc
     6481 cccggagtgc tcgttaagac caggcttgag acgggtgcta gtttcgagat ccggacctgc
     6541 ttttttcgcg cgcgggtata aaaccgcccg cgcgcgaggc gaacaggtct tcacgggccg
     6601 tcttacctcc ccggcgcgaa cgttgggatc gtgagacata acccagatgg aaatatgtcc
     6661 cggaaacgca cggaatctgg tccaggcgcc ggaccgttac ttcacgggct tggcctatcc
     6721 aggggcaggc aagccgacct gacgagagac ggaacgtagc cgcggactcc gcgtaggtaa
     6781 cgtaatcctc ctgaggagcg ccacccgcca acgcgcgata cttcctgtca gaatcggata
     6841 cacggtgtcc tgcaggtccg ggtgagcccg caaccttttc tggtcagccc cccgaccggg
     6901 gcccactggc cccacaccgg cgagaaatgg cccgaaggat gtatacggaa ccacccgatt
     6961 ctgcgacgag ggactaggca ggggggctag ttacgtacta gacgcctgga gaggagacgt
     7021 ctctagccgt cccagagggg gccccgacgg gtccttctcc gcctctcttg ccagagcgcg
     7081 atctgggcgg cgaacctgtc gccgtcgagc ccgaggtagt cggcgtaggc tttgacgaaa
     7141 ccgcgaacgt agatcggctc gggcatcgcc gagtggtcgt cgctttccag ccgctgcagg
     7201 taatccttgc ggatcaaggt agccctctcg gcctcgtcca gggatacacc ccggctgatt
     7261 ctagcgcgct ccagcagatg acctattcca ggtctgtcta cctgcggggc gtagcttttg
     7321 tcgatctctc tcatctcaga cctccgagcc tttgcctgtg acgggtgtcg tgcgattgcc
     7381 gggcggttcg ggccgttcct cccccctgtc cacgaactgg agcagcacgt cgagcccggg
     7441 gtcagggtcg ggggagttgc cagcgtcgct ggttcgtacc agaagcgctc cttcttcgag
     7501 gcggacctcc atccagcggc ccgcggcggt cctgagcgcg ggcgtgacgc ggctgcacac
     7561 tacggcgtcg gggcgtagcc ccggcgcctc cccttccagg gcgtcaggcc tgacagttat
     7621 cacctcgacg tgcggacgca gcacacggag ggccccggca aagacttcct ggtgcgagcg
     7681 gggttcgttt gccatcagaa cacgcatcgt tactcccctc gtgaggacgc ccgccggtgt
     7741 ccggtgggtg ccagggagag gcccgtaaac aggcggttct tgccccgacg cttggccctg
     7801 tacatggcct cgtctgctgc gtccagaagc gtcgccgggt ctgtggcgtc gccggggcac
     7861 tgcacggccc ctgcgctgag acgcagcccg aggccgagcg attctcccga gcgttccgcc
     7921 accatctccc gcatctcctc gaagaaccgt cgggcctcgg ggagcgaggc cccgggcaac
     7981 atcaccagga actcatctcc gccgtggcgg gcgaccacgt tcacgtgccc ggagcacgcc
     8041 cggagcacgt cggcgaccag ttgcagggct tcgtcgcccg cccggtgtcc gtgggtgtcg
     8101 ttgatgagct tgaagtcgtc gacgtccacg aagagcacca tgaaacgctc gcccgtgcgc
     8161 cgtgccctct cgaactcctc cctgaagcgc gtctcgaagt ggccgcggtt gtagaggccc
     8221 gtcagcgggt cgagcgaagc ttcatgctgc agacgctcct ccaggtccct gattctggcc
     8281 gaggcctgct cgctctgcgt tctcgtgcgt tcgcgcaggc cgtaccggca ggcgaacacg
     8341 agcccggcat aggtgggcgt ctggatggcc aggtacccga tcaggctgga ggtatcgggg
     8401 gcgtagagtt gaggggtgag gctggcgagc agcgtgacgg ccacgcagag cgcggctacc
     8461 cccgttgaga agtagccggc gcagaagacc accacgaaga ggtagaagat cgagagcgga
     8521 ctctgccagc cgccggagaa gtacacggcg agggttatga gcacgatggc ggctgagacg
     8581 gggatgagca gaacgttccg cccgaaacgg tgccagggaa agaaccacac ggaccacacc
     8641 aggatcccac ccgccagcac caccctgtcg atcgcgaacg tgttcccggc tgggagctcg
     8701 aaccatcccc cgtggacgaa caagagcaag gcgatggcgc ccaccacgta cagcaggccg
     8761 ccgagcgtct ttaatcggaa gtccaggtgc ggagacagag caggagagat attgtgtgac
     8821 gggccgaggt tggccacacc ctccgtgagg ttggaggaac gggcgcgctg ctccggcccc
     8881 ttatcgtcat gagatggtcg gttgcgcttt tgccaggcgt tcatgttttc tccccgctct
     8941 accgtacgtc aggacaacgt gggacgtcgg gcgcacgaag gttgcgccca gagcgaggag
     9001 gatcagccgg ggcgttcgag tgccggccgc ggcaccgttc cggcccgcag caacgcgccg
     9061 taggcacgat accgcccccg ccacagaagg gcggagaggc agcagaagat cacggcgagc
     9121 agtggaacat ggccctcagc gtaggaagaa gcgccgagaa cggcaccgct catggtgaga
     9181 ccgaagccgg agaaggggga tgggagcggt agtggtggcg cccgctgcgt aatggcacct
     9241 ccgtgcgggg cctcgtcagc gaaccggcgc gcgccgtccc gcatcgggac gagaccatcc
     9301 tggccctgct gaaccacata actaccctcg tcccagaggt agccgaagaa gtactctgta
     9361 gatccgaagg cggcctcaga cgcggcccgg acccgatatt cctccggctg ccgcactcca
     9421 cccgggggcg cggctggagg agccgacccg acgacccgag aactcccgga ggaaccctca
     9481 gcatcctctc cgcgaacggc agcaggaggc ggggcggcgg tatcggacga ggtctcggcg
     9541 ggagcgggga acgcttcagt agaacgtgcg tcctggccgg gctccttgcc gttcgatgtc
     9601 gcgagggtcg ccctgttgcc ggggtcctgc gtcaggcccg tatccgaggg tagaccacca
     9661 gccggggtgt ttgtggcccc gccgtcgcta ccgcttcttg ctatcccggg atccttcccg
     9721 accgcggcct gcggctgacc gcccccgacg tctggttcct gtaccgtgcc ccacccggat
     9781 tgcacttccg gctgtgccgg cgtcccgtcc cgaccgttct ccgtcggaga acctacctgg
     9841 tccggcagcc caccaggctc gcccggctgg gtttgggatc ccttaccctc cggtcggccg
     9901 gtgtcctcgc caccccggaa gtctaccctg gggttctcgc tgggctcttt tacgacctca
     9961 ggtcttggcg tgggttgctg atcggtccgg ggtggctggc cccggtctac gggtttttcg
    10021 gggtaagccc gttcggggcg ctcggtcgga ccgatggcac cttgtgaggg cttctggggg
    10081 ggatccggcg gcgcgtgcgc gggcctctgg ggtggaggac cccgcctgcc cggcgtcgcc
    10141 gcaggcttct cctggccgat cggggcgcca ggctccgcag gcgggagtgg tgtgggtcgt
    10201 gatgctggcg gctgacgttc ggttcggggc acgcccgatg gtgcggccgg ccccggttgg
    10261 acatgccccc cggaaggccg cgagacaggt gcgacgttgg cgggtgcgac gcttgccgtc
    10321 cgccctgccc ggggcgccgc tggtctcgac tggggcacgg cctccgggcc ggctaacctc
    10381 tggggggttg cgggaccggc gggagcctgt acccccgctt gctgctgcgc cagggacgcc
    10441 ctaccggtac acaacagcag gcccagcgcc accacgatgc agatcgtggc cagagccaag
    10501 cggttacgcg cgaaagtgct gcccatggca cttccaacat cggcgggatg aagcagcctg
    10561 tcgtgatcgt ccataatccc caacgccgtc atacgatcca aggactggca gactacccgg
    10621 cctgttctcg gtggttcagc ccaccacctt cgctcgacat tacaggcccg ccagggagct
    10681 ttgtcaaatg ctccccgccc ccgccgccgg actctagtac ccgatactag cacttcggtt
    10741 cgggcccccg tacagccgga attctgaccc tgagcgtggg cctcgaagca ctgcgttcgt
    10801 cttgaatgcc ttctttcgcg gccacccctc tcttccggag tccccacccg gcatgccggc
    10861 atagtagcat agtgcgtgga agaagctatt gtatcctgga tcgggggtga gaggtttgca
    10921 cgtatacagt gacccggcct cggggcgcca cgtgaacgag ctggcaggcg tcgagagccc
    10981 ggagaggttc cccgcggcca tcgcgggggt cgaggaggca caccggggcg gtgtacccgt
    11041 ggaatggcgt tcgtgcgccc ccgctggcga ggatgacctc gccgccgtac acgacgcggc
    11101 ctacctcgac ggcgtcaggg agatggcccg ggcgggaggc ggctatctca gcccggacac
    11161 cgccctggga aaaggttcct gggaggctgc ctccctcgcc agcggggccg cctgctcggc
    11221 cgttgatagc gccctcgccg gcgagacggc cttcgccgtt gcgcgccctc ccggacacca
    11281 tgccgggcga gactacggga tggggttctg cctcaccaac cacgccgccg tcgccgctgg
    11341 ccacgccctc gcgggcggga tggagagggt cgccatcctg gactgggacg tacaccacgg
    11401 caacggcacc cagggcatct tctacgacga cgacagggtc ctgtacctct cgatgcacca
    11461 gagccctttc taccccggca cgggtgaggc gggcgaggtg ggggagggca agggccgtgg
    11521 tttcacggtc aacgtgccgc tgcccgccgg ctccggggaa gacgtctacg cggctgtctt
    11581 caccggggtc ttcgcgcccc tgatcgggga gttctcgccg ggcctgatcc tggtctccgc
    11641 gggcttcgat gcccaggcgg acgaccttct cgggggcatg gccctctcca gcgacgcctt
    11701 cgcacgcttc gccgcgttcg tcacgcagct ctcccgcgag atcggcgccg cgccgcccgc
    11761 cttcgtgctc gaaggaggct acaacctgcg ggcccttacc gagagcgtgg ccgcctgcat
    11821 tcggggggct acggatgagg cacccgactg gcggtacgac ggtggggtca ggcaggtaga
    11881 gaagagccgt gaggtcctcg ccccgttctg gcggggtgtc ggctgatccg gcatgaggct
    11941 cttccgctct ccgccgttcg aggaccgtag ggacgcggga cgtaagctcg ccgcgaagct
    12001 gtcccgtttc aggggtgccg ggtgcgtggt cttcgggctg ccgcggggtg gggtacccgt
    12061 ggcgtacgag atctcgcgct ctctgggggc gccgctcgac gtgttcgtct cgcgcaagct
    12121 cggggcgccg ggccagccgg agttcggcat cggggccgtc gcggccggcg gcgtgcgcat
    12181 cctgaacacc gacgtcataa agcgcctcgg catcacagac gagtacgtgg accggatcac
    12241 tgcccaggag gccgccgagg tgaaccggag gctccgctat ttccggggcg gtaggcccga
    12301 gccggaggtc gccggccgga ccgcgatcct ggtcgacgac ggcctcgcca ccggcgtgac
    12361 ggcccgcgcc gcactggaag ccctcaagct gcgcgggccg gagcgtctga tactggccgc
    12421 accggtctgc gccgcccaga cggtggggct cttcgcctcc ctggtggacg aactggtgtg
    12481 cctgacctct ccctctgacc tgggggccat aggcctctgg tacaggaact tcgaccagac
    12541 cacggaccag gaggtcgtgc agctgctgcg ggaggccaga gaagaactcg gctgacccgt
    12601 actgttgcta cccggatgat gggagagagg cacccatcca gtacaattag agcggtcagc
    12661 catcagcaaa acaacaagct gaaggctgaa agctagaggc tagaagctag aaactcggct
    12721 tctgggagag gagagaacca tgtgcggcag gtacacgctt tctactccgg cgggcaggct
    12781 ggccgaggag ttccagttgg atagcaccgt ggagattccc ccgagctaca acgtggcccc
    12841 gacacagcag gtcgccgcgg tcctcgagga cgagggagga cgacgcctgg agatgctccg
    12901 gtgggggctc gtgccctcct gggccgacga cccagacatc gggtcgcgca tgatcaacgc
    12961 ccgcagcgag acggcgccgg agaagccttc cttccgcagg gctttccggg ggcgccggtg
    13021 ccttatcccc gccgacggtt tctacgagtg gaagcgcgag aatgggggca agcaacccta
    13081 ctacttccac atgcaggacg ggcatccctt cgccttcgct ggcctctggg agagctggga
    13141 caagggagac ggaacgctca ggacctgcac gatactcacc accagggcaa accccgttct
    13201 gcaaggaatc cacgacagga tgcccgtgat cctgccccac gacgcctaca acgcctggct
    13261 cgaccctgac gccgaccggg aggagctcgg ggagctcatg atcccctacc cagaaggcga
    13321 cctcgaggcc taccccgtca gccgtttcgt caacagcccg cgcaacaacg acgaacgctg
    13381 tatagaaccg gccgcttgag cctggaggtc ggggccgagg tcagggaggc cctgcagggg
    13441 ggggagccgg tcgttgcgct ggaatcgacc ctgatctccc acggtctgcc ctatccgcag
    13501 aacctcgagg tcgccctcga ggcggagggt atcgtgcggg ccgaaggcgc cgttcccgcc
    13561 acgattgggg tcgtcggcgg gatcgccagg atcgggctcg acggggctga gctggagctg
    13621 atggccaccg ccgagggcat cccgaagctc tcggccaggg accttcccgt ggcactcgcc
    13681 aaggg
//
LOCUS       DBNGVD010000227         6536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3326340, whole genome shotgun sequence.
ACCESSION   DBNGVD010000227 DBNGVD010000000
VERSION     DBNGVD010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3326340"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1912)
                     /locus_tag="AC16CM_06365"
     CDS             complement(41..1912)
                     /locus_tag="AC16CM_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139488.1"
                     /translation="MKVDLNNAWEVTALLQRFEPVIRSTRGDVFYPMDVEPYVKNCSL
                     WVQKPGREARRVVPDGKLSLEALAQQPRDEAGAVHFLKLSDAEEMSRNEPEGRLQMFR
                     RHARDRKESREAFRAGRGRLARVGYFSRFVDALYSIALLARGRVPGESAAAALEAYEH
                     IMAEHEHYRYHGRVLRQNGWVVLQYWLFYPFNNWRSGFFGANDHEADWEKVFLYLSES
                     EDGEVRPEWVAYAAHNYTGDNLRRRWDDPEVVKVGEHPVIFVGAGSHASYFAQGEYLT
                     ELTVPLPGPVARVASRLRSFWKARLGQYEEDEEADDGSGYLRIPFVDYARGDGLSVGE
                     GGDRAWDEPRLLAHPIPDWVSGYRGLWGLYARDPFEGEDAPAGPMYNRDKTVARAWYD
                     PVGWAGLDKVSPPTEAIETVLEQREDIAARCETLRAEIEEKSRELKKLGAGLSAMRGR
                     SHLDGPRAQGASRAAELSGEIDRLRARLATDEAVSESLLEYAGRLRGGEHEPVRAHIG
                     RAHTPASEDELRISRVAEAWAAVSVSLMIISFVAIALLERQHLISMLVFSIAFFSFVE
                     AGFRGRLVNLVISANVGLAVVATLILIYEFFWPLVVATVLVVGLYVLWDNLRELRSR"
     gene            complement(1902..2330)
                     /locus_tag="AC16CM_06370"
     CDS             complement(1902..2330)
                     /locus_tag="AC16CM_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139489.1"
                     /translation="MEPNKAAYRTVKLIGLLLILEVVALVAIGSFEFFQIRWGGLNLE
                     GARHDEVERALYALFIPSGILTLMSAFSFLFMRRRGWLLAAIAQALSLAVSLWLYSWF
                     QPTYIYPIMAYCVLMILFLNSHDVRVVFLSRQDSGASVEG"
     gene            complement(2471..3835)
                     /locus_tag="AC16CM_06375"
     CDS             complement(2471..3835)
                     /locus_tag="AC16CM_06375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3139490.1"
                     /translation="MDPLAELAEALPEGRVVSGGDDLERHAGGVFTYHAPVRPEAVVY
                     PHDRGEVLEILRFAHEHRVPVVPFGQGSSLEAHTLPVRGGISLDLAMMDRVLEIRPDD
                     FIVRVQPGLTHKRLNAELADHGLHFPVDPGWDASLGGMAGTNASGTNAVKYGVMRDQV
                     LGLEVVLANGTVMRTGGMAIKSSAGYDLTGLFVGSEGTLGVFTDLTLRLYPLPEHTVA
                     ARAVFPDVEAAGRAAVAMIRTGMAVGRVELVDARTIEAVNAYKETDYAVAPNLFLEFS
                     GSEAGVEYDVAAARERSAAGGCSLFEVEADEAARERLWEARHEAAIAIQSLHPGMGMM
                     STDVCVPISDLPGALRHARDTIESRGLEGAILGHVGDGNYHAVFPVDPHDAWDQERAE
                     AVNTEIVDYALQRGGTCTGEHGIGVGKTDHLQKEHGDSLPFMRDIKSIADPNGIMNPG
                     KIFA"
     gene            complement(3839..4657)
                     /locus_tag="AC16CM_06380"
     CDS             complement(3839..4657)
                     /locus_tag="AC16CM_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3139491.1"
                     /translation="MKLVTYSVDGSGPRVGALEGEEIRPLGHEDMIEFIEYGGSPEPG
                     EDTVPLAEARIHAPIAGPEKVIGIGLNYEDHAAETGADIPEKPIVFAKYANTVIGPGE
                     TITIPPITEQADYEAELAVIIGKTARNVSESEALDYVFGYTNANDVSSRDLQFSEGGQ
                     WTRSKSIDTFCPLGPYIATADEVGDPQDLSVRCILNGEVMQDGTTSKMIFSVAELVSF
                     LSQGMTLVPGDVIVTGTPPGVGSARDPQVWLKDGDEVTIEIEGLGSLTNPVETE"
     gene            4814..>6536
                     /locus_tag="AC16CM_06385"
     CDS             4814..>6536
                     /locus_tag="AC16CM_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3139492.1"
                     /translation="MGNRLFVVFAALVVALPVLVLSGGGLARASGSSDEALARPSYRH
                     PVQNEQFYFVMPDRFENGSASNDTGGLAGGTSDEDVLRHGYDPELKGYYHGGDIAGLR
                     EKLDYLDDMGVSAVWMTPMFKNKPVQGDGTIAGSSAGYHGYWITDFTRIDPHMGTNRE
                     LQALIDEAHDRGMKVFFDIITNHTADVIRYKEGQYSYISKAQEPYRTASGKPFDDRDY
                     AGTEEFPELDPQVSFPYTPVVPRAERDVKEPDWLDNRIYYHNRGDTDFQNEDEDQQYG
                     DFVGLDDLFTEHPRVAQGMVDVYNDWVDYGVDGFRIDTVKHVNTEFWQKFGPEVLAHA
                     KATGNEDFFMYGEVYSYDPSFTSIYTTEARLQAILDFPFQGAAREFASQGEPTRDLRD
                     FFRQDDYYTDADSNAYSLPTFLGNHDMGRFGYFLEQDNDGATDRELLKRDRLGHALMY
                     FARGMPVVYYGDEQGFTGGDPGNDQLARQDMFPTRTEEYKDDDQIGSGATPADNNFDR
                     DHPLYQILQDLAAARQEHKALRRGAQIQRYSDDQAGIYAFSRIARGEQVEYVVALNNA
                     ETTKTATF"
BASE COUNT         1180 a         2176 c         2055 g         1125 t
ORIGIN      
        1 tccttccagt ttctggctga tggctgatag ctgatagcta ttatctactc ctcagctctc
       61 gcaggttgtc ccagaggacg tagaggccga ccactagcac ggttgcgacc acgagtggcc
      121 agaagaactc gtagatcagg atcagcgtgg cgaccacggc caggccgacg ttggcgctga
      181 tcaccaggtt caccaggcgg cccctgaagc cggcctccac gaaggagaag aaggcgatgc
      241 tgaagacgag catggagatc aggtgttgcc gctcgagaag cgctatggcg acgaagctta
      301 tgatcatgag gctgacgctc acggcggccc aggcctcggc cacgcggctg atcctgagct
      361 cgtcctccga ggcgggcgtg tgggcgcgcc cgatgtgggc gcggacgggt tcgtgctcgc
      421 cgcctctcag gcgtccggcg tactccagga gcgattcgga gacggcttcg tcggtggcga
      481 gcctggccct gaggcggtcg atctcaccgg agagctcggc cgccctgctg gcgccctgtg
      541 cgcggggacc gtcgaggtgg gacctgccgc gcatcgccga gaggccggcg ccgagcttct
      601 tgagctcgcg gctcttctcc tcgatctcgg cccttagcgt ttcgcaccgc gcggcgatgt
      661 cttccctctg ctcgaggacg gtctctatcg cctcggtcgg gggagatacc ttgtccagcc
      721 cagcccagcc cacggggtcg taccaggcac gggcgacggt cttgtcgcgg ttgtacatcg
      781 gtcccgccgg cgcgtcctcc ccttcgaagg ggtccctggc gtagaggccc cagagcccac
      841 ggtagcccga gacccagtcg gggattggat gggcgagcag cctgggctcg tcccacgccc
      901 tgtcgccccc ctcgccgacg ctcaggccgt ccccgcgggc gtagtcgacg aaggggattc
      961 tcaggtagcc agacccgtcg tctgcctcct cgtcctcctc gtactggcca agcctggctt
     1021 tccagaagga tcgcaaccgg ctcgccaccc ttgctacggg tccggggagg ggcacggtca
     1081 gctccgtcag gtactcgccc tgggcgaagt agctggcatg ggagcccgcg ccgacgaaga
     1141 taacgggatg ttcccccact ttcacgacct cggggtcgtc ccagcgacgc ctgaggttgt
     1201 cccccgtgta gttgtgggcc gcgtaggcca cccactcggg cctgacctcc ccatcttcgg
     1261 actcggagag gtagagaaag accttctccc agtcggcctc gtgatcgttc gcgccgaaga
     1321 acccgctcct ccagttgttg aagggataga agagccagta ctgcaagacc acccaaccat
     1381 tctggcggag cacacgcccg tggtagcggt agtgctcgtg ctcggccatg atatgctcgt
     1441 aggcctcgag ggccgccgcc gccgactccc cgggaaccct gccgcgggcg aggagcgcga
     1501 tggagtaaag ggcgtcgacg aaacgcgaga agtacccgac gcgggcaagg cgcccccgcc
     1561 cggcccggaa ggcttccctc gattccttgc gatccctggc gtgcctgcgg aacatctgca
     1621 ggcgcccttc gggctcgttc ctactcatct cctcggcgtc gctcagcttc agaaaatgga
     1681 ccgcgccggc ctcgtcccga ggctgttggg ctaacgcctc aaggctcaat ttgccgtcgg
     1741 gcaccacgcg ccttgcctcc ctgccgggct tctggaccca caggctgcag ttctttacgt
     1801 atggttccac gtccatcggg tagaagacgt ctcccctggt ggaccggatc accggctcga
     1861 agcgctggag cagggccgtg acctcccacg cgttgttcag gtcaaccttc aacggaggcc
     1921 cccgaatctt gcctggagag gaacaccacc ctcacgtcgt gggagttgag gaacaggatc
     1981 atcagcacgc agtaggccat gatgggatag atgtaagttg gctggaacca cgagtagagc
     2041 cagagcgaca cggccaggct caaggcctgc gcgatggcgg ctagaagcca tccccgacgg
     2101 cgcatgaaca ggaagctgaa cgccgacatc aacgtcagga tgccagaagg tatgaacaag
     2161 gcgtagagcg ctctctccac ctcatcgtgc cgcgccccct ccaggttcag ccctccccac
     2221 cggatctgga agaactcgaa gcttccgatt gcgacgagcg cgacgacctc caggatcagg
     2281 agcaacccta tcaacttcac cgtccggtac gcagctttgt tcggttccat caataccccc
     2341 tggacctcct ctccaagacc tttattctac agcccgcagc cgccagagac cgcctcgagt
     2401 agggagcctt cagctttcag ctatcagcga ttagctatcg gcttttcgtc ttccgcggag
     2461 ggggcgatta tcaggcgaag atcttgcccg ggttcatgat gccgttcggg tcggctatgc
     2521 tcttgatgtc gcgcatgaag ggcagggaat cgccgtgttc tttctgcagg tggtcggttt
     2581 ttccaacgcc gatgccatgc tcaccggtgc aggtaccgcc gcgctgtagg gcgtagtcca
     2641 cgatctcggt gttgaccgcc tcggcccgct cctggtccca cgcatcatgc gggtccacgg
     2701 ggaagaccgc gtggtagttt ccgtccccga cgtgtcccag gatggccccc tccaggccgc
     2761 gggactcgat ggtgtcgcgg gcgtggcgca gggccccggg gaggtcggag ataggcacgc
     2821 acacgtccgt gctcatcatc cccatccccg ggtggaggct ctggatggcg atggctgcct
     2881 cgtggcgggc ctcccagagc ctctcacggg ccgcctcgtc tgcttcgacc tcgaaaaggg
     2941 agcatccccc cgccgccgac ctctctcggg ccgccgccac gtcgtactcc acgcccgcct
     3001 ccgagccgct gaactcgagg aagaggttcg gggctacggc gtagtccgtc tccttgtagg
     3061 cgtttaccgc ctctatcgtg cgggcgtcga ccagctcgac gcgccctacg gccatcccgg
     3121 ttcggatcat ggccaccgcg gcacgccccg ccgcctcgac gtcggggaag acggccctgg
     3181 cggcaaccgt gtgctccggg agcgggtaga gacgaagggt cagatccgtg aacacgccca
     3241 gggttccctc tgatcccacg aagaggccgg tcaggtcgta gcccgccgag gacttgatgg
     3301 ccatcccgcc cgtccgcatc accgtaccgt tcgcgagcac cacctcgagg ccgaggacct
     3361 gatcgcgcat gaccccgtat ttgaccgcgt tcgtcccgct cgcgttggtg ccggccatcc
     3421 cgccaagcga ggcgtcccag ccgggatcca caggaaaatg gagcccgtgg tcggccagct
     3481 cggcgttgag cctcttgtgg gtgaggccgg gctgaacacg gacgataaag tcgtccggcc
     3541 tgatctcgag aacacggtcc atcatcgcca ggtcgaggct gatgcctccc cggacgggca
     3601 gggtatgggc ctccaggctg ctcccctgcc cgaaaggcac gacggggacg cgatgctcgt
     3661 gggcgaagcg aaggatctcc aggacctcgc cgcgatcgtg ggggtagacc acggcctcgg
     3721 gccgaacggg cgcgtggtag gtaaagacac caccggcgtg ccgctcgagg tcatcgcccc
     3781 cggagacgac gcggccctcc ggcagggctt cggccagctc cgcgagcggg tccaccggct
     3841 actccgtctc gaccgggttg gtgagggaac ccagcccctc tatctcgatc gtcacctcgt
     3901 ccccgtcctt caaccacacc tgcgggtcgc gggccgaccc gacaccgggg ggcgtacccg
     3961 tgactatgac gtccccaggc acgagcgtca tgccctgcga gaggaacgac accagctcgg
     4021 cgaccgagaa gatcatcttc gaggtggtcc catcctgcat gacctcgccg ttgaggatgc
     4081 atcggacgga aaggtcctgc ggatccccca cctcatcggc ggtggcgatg tagggtccca
     4141 gcgggcagaa ggtatctatg gacttgctcc tcgtccactg accgccctcc gagaactgca
     4201 ggtcgcgcga ggagacgtcg ttggcgttgg tgtagccgaa cacgtaatcc agggcctccg
     4261 actccgagac gttgcgggcc gtcttaccta tgatcacggc gagctcggcc tcgtagtcgg
     4321 cctgctcggt tatgggaggg atggttatgg tctcgcccgg ccctataact gtgttggcgt
     4381 acttggcgaa gacgattggt ttctcgggga tgtcggctcc cgtttcggcc gcatgatcct
     4441 cgtagttgag gccgatgccg ataaccttct cgggacccgc gatcggcgcg tggatgcggg
     4501 cctcggcgag cggcacggtg tcctcaccgg gctccgggct cccgccgtac tcgatgaact
     4561 cgatcatgtc ctcgtggccg aggggcctta tctcctcacc ctccagggcc cccacccgcg
     4621 gcccacttcc gtccacggaa tacgtaacca gcttcaaatc gtcgtccctt cctcgcgtac
     4681 acgacgcaac gatacgcatc ttagcaggga ctgacgtgag gccggggatc ctttatcggg
     4741 gctccaggtc atgctaccgt agttatgaac agccctggag accggctccg gggcagagag
     4801 gggcggagga cgggtgggta acaggctttt cgtggtattt gcggcgctcg tagtggcgtt
     4861 acctgtgctg gtgctatccg ggggcgggtt ggcgcgggcg agcgggtcat cggacgaggc
     4921 gctcgcccgc ccctcgtaca ggcatcccgt ccagaacgag cagttctact tcgtcatgcc
     4981 agaccgcttc gagaacggca gcgcctccaa cgacaccggc ggcctggccg gcggcacctc
     5041 cgacgaggac gttctgcgcc acgggtacga tcccgagctc aagggctact atcatggcgg
     5101 cgacatcgcg gggttgcgcg agaagctcga ctacctcgac gacatggggg tatcggcagt
     5161 atggatgacg cccatgttca agaacaagcc cgtgcagggc gacggcacca tagccggctc
     5221 atctgccgga tatcacgggt actggatcac cgacttcacc aggatcgacc cgcacatggg
     5281 caccaaccgg gagctccagg ccctgataga cgaggcccac gacaggggca tgaaggtgtt
     5341 cttcgacatc atcaccaacc acacggccga cgtgatccgt tacaaagagg gccagtacag
     5401 ttacatcagc aaggcgcagg agccgtaccg tacggcaagc ggcaagccct tcgacgaccg
     5461 tgactacgcc ggcacagagg agtttccgga actcgacccg caggtcagct tcccctacac
     5521 gccggtcgtg ccccgggcgg agcgcgacgt gaaggagccg gactggcttg acaaccgcat
     5581 ctactaccac aacaggggcg acacggactt ccagaacgag gacgaggacc agcaatacgg
     5641 cgacttcgtc ggcctcgatg acctgttcac cgagcacccg cgcgttgccc agggcatggt
     5701 cgacgtctac aacgattggg tggactacgg tgtagatggt ttccgcatag acaccgtcaa
     5761 acacgtcaac accgagttct ggcagaagtt cggccccgag gtgctggccc acgcgaaggc
     5821 gaccggcaac gaagacttct ttatgtacgg ggaggtctac agctacgacc cgtcgttcac
     5881 aagcatctac accaccgagg cgaggctgca ggcgatcctg gacttcccct tccagggcgc
     5941 ggcgagggag ttcgcctcac agggcgagcc caccagggac ctgcgcgact tcttcagaca
     6001 ggacgactac tacacggatg ccgactcgaa tgcttacagc cttccgacct tcctcggcaa
     6061 ccacgacatg ggtcgcttcg gctacttcct cgaacaggac aacgacggcg cgacggacag
     6121 ggaattgctc aaacgcgaca ggctcggcca tgccttgatg tacttcgcac gcggtatgcc
     6181 ggtcgtgtac tacggcgacg agcagggatt cacgggaggc gaccccggca acgaccagct
     6241 cgccaggcag gatatgttcc cgaccaggac cgaggagtac aaagacgacg accagatcgg
     6301 ctcaggcgcg acgcccgccg acaacaactt cgacagggac cacccgctct accagatcct
     6361 acaagacctc gccgccgcaa ggcaggagca caaggcgctc cgcaggggag cccagatcca
     6421 acgctacagc gacgatcagg ccggcatcta cgccttctcg cgcatagccc gtggggaaca
     6481 ggtcgagtac gtggtcgcgc tcaacaacgc cgagaccacg aagacggcga ccttcg
//
LOCUS       DBNGVD010000228         7708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_412986, whole genome shotgun sequence.
ACCESSION   DBNGVD010000228 DBNGVD010000000
VERSION     DBNGVD010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_412986"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC16CM_06390"
     CDS             complement(<1..387)
                     /locus_tag="AC16CM_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3139493.1"
                     /translation="MIGTRIEGGRGIIELRWNNIPLPEPHLAGILVSGVLHLTSSRRL
                     FGDRPLYRSAGWSLVVAGFTISALAVRAASEVDLERPSELISSGPYAICRNPMYVGWT
                     LLYFGIALASRNVWMVALLPAVAALTH"
     gene            complement(368..910)
                     /locus_tag="AC16CM_06395"
     CDS             complement(368..910)
                     /locus_tag="AC16CM_06395"
                     /EC_number="1.13.11.20"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0017172 - cysteine dioxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HIR3139494.1"
                     /translation="MSFTPYALDAGLLPLEVALPRAVSVLSGLAEDDDFLESHVLPFL
                     EKPAEDWYVACREDSPDGSTSLQVFVWPPGSATEIHDHSSWGAFRCVVGSVQEERYER
                     TDDGSRPDHARLTKVWQLQWRKEDGTSTVLPYEGGIHRVGNTTAEPAISVHLYGPRLG
                     EIDGRDYDLSRDYVCDRHED"
     gene            complement(885..2060)
                     /locus_tag="AC16CM_06400"
     CDS             complement(885..2060)
                     /locus_tag="AC16CM_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR3139495.1"
                     /translation="MNTRREVKMRSRRTALEPERVDTVIIGGGQAGLATGYHLAKRAM
                     PFVVLDASERTGDSWRKRWDSLRLFTPARFSGLPGWRFPAPAISFPTKDEMADYLEAY
                     AARFDLPVRTGVKVDRLFREGERFVVQSGARRFEAKHVVVATGAHRVPSVPAFAGELH
                     PNIVQLHSSQYRRPSQLGEGAVLVVGAGNSGAEIAFELSRTHPTRLSGKPSGQIPVRH
                     GPTAARFVFPVVRFAAHHVLTLDTPVGRKVRPQFISHGAPLIRVKVKDLAAAGVEQVP
                     RTVGAKDGRPALEDGRVLDVSNVVWCTGYKEEFSWIDLPIFDRDERPVHERGVVVGES
                     GLYFVGLPFQYAASSDVIPGVGRDTEYVAKHITADVSHGRYQERMGKDADELHAIRS"
     gene            complement(2191..3831)
                     /locus_tag="AC16CM_06405"
     CDS             complement(2191..3831)
                     /locus_tag="AC16CM_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3139496.1"
                     /translation="MVDAAGELVHGRESYARRAWMDAYTSLSQADRTTPLGAEDLDLL
                     ATSAYMIGHDDECLGSLERAYHVHLESGEVLRAVRCAFWVGITLALHGEVGRASGWLS
                     RAQRLVDREADDCVEQGYLMIPAMLRHEATGDWEAAYAIAADAVEIGERFGDADLLAL
                     TVHEQGLALIHQGRVEEGLGLLDEAMVAVTAGEVSPIVTGIVYCSVISGCQKVYALRR
                     AREWTAALTRWCEDQPEMVAFGGTCMVHRSEIMALHGAWQDALEEARRARQRCERANN
                     PSSAAQAHYSQGEIYRLQGKFAEAEEAYRNASRSGWEPQPGLALLRVAQGNEDAAAAA
                     IRRAVGEATDPLKRARLLPAYVEIMLSSGDVREARRASDELREISVRYESAMLDAMVA
                     HAKGAVDLAEGDPRAALLALRRAWQMWQEIEVPYEVARVRVLLGEACRALGDEDTAVL
                     ELEAAHGIFEQLGAAPELARLESPPRDSGSVASHRLTSRELEVLRLVADGETNRAIAV
                     ELVVSERTVDRHVSNIFAKLGVSSRAAATAHAYRRQLI"
     gene            4201..4356
                     /locus_tag="AC16CM_06410"
     CDS             4201..4356
                     /locus_tag="AC16CM_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139497.1"
                     /translation="MKGARSGYWMALPALLAILALVAYPLAFTTYYPTGGPAWLLARS
                     QRHHAFA"
     gene            4685..4951
                     /locus_tag="AC16CM_06415"
     CDS             4685..4951
                     /locus_tag="AC16CM_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3139498.1"
                     /translation="MADVHVYVNRTAAHEGTNLEDIEEALRQLDYVSHVEANPVGNVV
                     AVSFEGGKTEQEGIKDAVESIGYEVSRFSKRSTIADQDRNLWDI"
     gene            complement(5164..6261)
                     /locus_tag="AC16CM_06420"
     CDS             complement(5164..6261)
                     /locus_tag="AC16CM_06420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139499.1"
                     /translation="MTEKKTAGASGDLGVMEGKGFYNRHSRPQRGAVSFGLPLLEHAV
                     GAVPLPDAGGVLQVADYGVAGGNNSMEPMRTIIDGVRQRSSEDLAVSVFHTDLPTNDF
                     DPLFTLLASPASYLQGTSNVFAYAGGKSFYERLFPDSHLHIGWNAIAIHWLSRVPATI
                     PDHIWSNRAAGTVKEAFARQSEGDWQAFLYHRSHELRPGGRLVVLGGASDDEGASGAE
                     GLMDMANAALQEIVEGDTLRPDEYERMVIPTYNRTLKEFETPFSPGQKGSPLELESSS
                     EVVLPDPFWPEYEQSGDARAFAADYEAFFRAAYGPSLFGALDADRTPQEHEQIADAFF
                     DNLREKVAADPATASCHWRVVLLLIAKGADA"
     gene            complement(6292..7122)
                     /locus_tag="AC16CM_06425"
     CDS             complement(6292..7122)
                     /locus_tag="AC16CM_06425"
                     /inference="COORDINATES: protein motif:HMM:NF017373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3139500.1"
                     /translation="MANALEALWTAISGFTPRLVGAVLVLVVAFLVARLLQRLGARLL
                     QRVGLDRLFESTGAAASLQRVGFGRSPSFVVAYVLFWGVLLAGVAAALSILGLASLER
                     NMDLVVALAGKAVVAVLILAGGLAAAGWLSNLAIIRSESAGLRGGDLFGRAVFAFVLA
                     IAFLLAAAQLGINISILVLLAAILFGAAGIVAALALGLGLVPLSGNIAAGRYVQGNGI
                     REGDVISVNGIEGTVEELGLASVTLRSADGRIHYVPNRVLLEGVVSKTAPGERGDREQ
                     "
     gene            7295..7367
                     /locus_tag="AC16CM_06430"
     tRNA            7295..7367
                     /locus_tag="AC16CM_06430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7328..7330,aa:Ala,seq:cgc)
BASE COUNT         1457 a         2517 c         2393 g         1341 t
ORIGIN      
        1 gtgggtgagt gctgccacgg ctggaagtaa ggcgaccatc cacacgtttc gcgaggcaag
       61 agcgattccg aaatagagca aggtccagcc gacatacatc gggttgcggc atatcgcgta
      121 gggcccacta gaaatgagtt cagagggtcg ctccaggtcg acctcggagg cggctcgcac
      181 cgccagtgct gatatggtga atccagctac cacaaggctc catccagcgc tacgatatag
      241 tggcctgtca ccgaaaagtc tccgggagct cgttagatgc agcactccgc tgacgagtat
      301 tccggccagg tggggctcgg gaagcgggat gttgttccat ctgagctcga tgatgcctct
      361 tcctccttca atcctcgtgc cgatcacaga cgtaatctcg tgaaaggtcg taatcccgtc
      421 cgtctatctc tccgagccgg ggaccgtaga gatggaccga gatggcaggt tccgccgtgg
      481 tattgccaac ccggtggatg ccaccctcat acggcagcac agtggaggtt ccgtcttcct
      541 ttctccactg gagttgccag accttcgtca ggcgggcatg gtctggcctg gaaccgtcat
      601 cggtgcgctc gtaacgttcc tcctggacag accccacgac gcaacggaag gcgccccagg
      661 atgaatgatc gtggatctcg gtggcagaac ccggaggcca gacaaacacc tgcagggacg
      721 tggacccatc aggagaatct tcgcgacagg ctacgtacca gtcctcggcc ggcttctcca
      781 aaaatggcaa gacgtgcgat tccaggaaat cgtcatcttc tgccagacca gataggacgg
      841 acacggcccg tggcagcgcc acctccaggg gtagaaggcc ggcgtcaaga gcgtatggcg
      901 tgaagctcat cagcgtcctt ccccatccgc tcctggtatc gtccatgcga tacgtctgcg
      961 gtgatgtgtt tcgcgacgta ctccgtgtcc ctcccaacgc cgggtatgac gtcggaagag
     1021 gccgcgtact ggaagggcaa ccccacaaag tacagcccgg attctccgac cactacgcca
     1081 cgttcgtgca ccgggcgttc gtcccgatcg aagatgggga ggtcgatcca ggaaaactcc
     1141 tccttgtacc ccgtgcacca gaccacgttc gacacgtcca ggacgcgccc gtcctctagc
     1201 gcgggacgac catcctttgc gccgacggtt ctcggcacct gctcgacccc ggcggcggca
     1261 agatccttca ccttgacacg gatgagcggc gcgccatggg agataaactg gggtcggact
     1321 ttccgtccta cgggcgtatc cagggtaagc acgtgatggg cggcgaaccg caccacagga
     1381 aatacgaacc tggccgccgt aggcccgtga cgaacgggga tctgcccaga aggtttgccc
     1441 gacaggcgcg tgggatgtgt gcgggacagc tcgaaagcga tctccgcccc ggagttgccc
     1501 gcgccgacca caagaacggc gccttccccg agctgggagg gacgacgata ctggctggaa
     1561 tgaagttgca cgatgttcgg gtggagctcg ccggcgaacg ccgggacact cgggacccgg
     1621 tgggcacccg tcgcaacgac cacgtgcttc gcctcgaaac ggcgggctcc ggattgcact
     1681 acgaaacgtt ctccctcccg aaagagtctg tccaccttga cgccggttct aaccggaaga
     1741 tcgaaccggg ccgcgtaggc ctcgaggtag tcggccatct cgtccttcgt ggggaaagaa
     1801 atggcgggtg cggggaagcg ccagcccggg agccccgaga agcgggccgg ggtgaacagt
     1861 cgcagcgaat cccaccgctt gcgccaggag tcaccggtcc tttcgctggc gtccaggacc
     1921 acgaagggca ttgcgcgctt cgccaggtga tagccggtag cgagacctgc ctgaccgcca
     1981 ccgatgatca ccgtatctac gcgttccggt tcaagggcgg tacgccgtga gcgcatcttc
     2041 acttcgcgcc ttgtgttcat cgatgcctcc tcgttgccag agggcgggtc tgcacaatcg
     2101 acagtctcct ccagaagagg gggcggtaca tcggcggaac tacccaactt atcctactgg
     2161 cgacgtgggt agaactaccc acgagggtgc ttagatgagc tgacgcctat aggcgtgggc
     2221 ggtggcggcg gcacgcgatg acacacccaa cttggcgaag atgttgctga cgtgccgatc
     2281 caccgtcctc tcgctgacga cgagctccac ggctatggcc ctgttcgttt cgccatcggc
     2341 gaccaggcgc agtacctcga gttcgcgcga ggtcagccta tgagaggcaa cagaacccga
     2401 gtcgcggggg ggcgattcta gccgggccag ctcaggcgcc gcgccgagct gttcgaagat
     2461 accgtgggcc gcttccagct ccaggacggc ggtatcctca tccccgaggg cacggcaggc
     2521 ttctcccaga agcaccctta cgcgtgcgac ctcgtaaggc acctcgatct cctgccacat
     2581 ctgccacgcc cggcgcagcg ccagaagggc ggcccgaggg tcgccttcgg ccagatccac
     2641 cgctcctttg gcgtgtgcga ccatcgcgtc caacatggcg ctctcgtagc gtaccgagat
     2701 ctcccgtagc tcgtcgctgg cgcggcgggc ctccctgacg tcgcccgagg agagcatgat
     2761 ctcgacgtag gcgggcagga gtctggcgcg cttcagggga tctgtggcct cgcccacggc
     2821 acgacggatc gcggcggcgg ccgcgtcttc gttcccctgg gcaacccgca acagggccag
     2881 gccaggttgc ggctcccacc cggaccggct cgcgttgcgg tacgcttcct cggcctcggc
     2941 gaatttgccc tgcaaacgat agatctctcc ctggctgtaa tgggcctggg cagcggacga
     3001 cgggttattg gcccgttcgc aacgctggcg ggcccgtcgc gcttcttcca gcgcgtcctg
     3061 ccaggcgcca tgcaatgcca tgatctcgga gcggtgaacc atgcaagtgc cgccgaaggc
     3121 caccatttcg ggctgatcct cgcaccatcg cgtcagggcg gcggtccatt ccctggcgcg
     3181 gcgcagcgca tacaccttct gacaacccga gatcacgctg caatagacga tgcctgtgac
     3241 gatgggcgaa acctcccccg ctgtcacggc taccatggct tcgtccagca gcccgagccc
     3301 ctcctcgacc cgcccctgat ggatcagggc gagcccctgc tcgtgcacgg tgagggcgag
     3361 cagatccgcg tcaccgaagc gctcgccgat ctcgacagcg tcggctgcga ttgcgtaggc
     3421 ggcctcccaa tcgcccgtgg cctcgtgccg cagcatggcc ggtatcatca aatacccctg
     3481 ctcgacgcag tcgtcagcct cacggtcgac cagccgctgg gcgcggctga gccagccgct
     3541 cgcacggccc acctctccat gcagcgccag ggtgataccc acccagaaag cgcatcgcac
     3601 cgcgcgcaac acctctccgg attcgaggtg cacgtgataa gcacgctcca ggctgcccag
     3661 gcactcatcg tcgtgaccga tcatgtacgc cgacgtggcg agcaaatcga ggtcctcagc
     3721 cccgagcggt gtcgtccggt cggcctgcga aagcgacgtg taggcgtcca tccacgcccg
     3781 cctcgcgtag gactcacggc cgtgtacgag ctcacccgcg gcgtctacca cggccacacc
     3841 aaccgtttcg agaaaacggc gggcaggatg ggtggcccga aggggaggta gtcatcaggc
     3901 cggcaaggcg tgcacccgtg accgaagtcc cccacgagat gccgcagctc gattcctacc
     3961 gccgggccct ccgcttccgc ggtaatcgcg ctcgtatgat gctctttgcg gcgcaggtta
     4021 tccatcgcac cgaagaacct cctagtccgc gggttattat aacgtgtgca tcaatcaaag
     4081 tgggttcccg atgcacgccg cgcgacaacc gtggaggcgt aaacggcgat ctgccccggc
     4141 cttggactga gggtcagggg tgagaagtgg cgtggggcaa tcagcagcat cgtaaaggtc
     4201 ttgaagggag ccaggtcagg ctactggatg gccctgcccg cgcttttggc tatactggcg
     4261 ctcgtcgcct acccgctggc cttcaccacc tactacccca ctggcggccc cgcctggctc
     4321 ctggcccgca gccaacggca ccatgcgttc gcctagcggg acgtagcatc tacctacagc
     4381 ccttcgagat gacccagcta gacagggatg ggaggtggga tcaggggccg ctggtctcct
     4441 ccctggagcg gcgcgagttt cccgccatcc tgatctggga aactgccgta cgatcagggt
     4501 gtctacaggg agtggtggac ccggtgctgc tccaggccat agacgaaact tcaggcccac
     4561 ccatgaatac ggcggaaacg tggtctagcg tcccaggccg gatgaggacg tcccctgata
     4621 atccgggtat attggacaag cgggccggag cgggcaggtt tacaccgacc gaaaggagat
     4681 cagcgtggcg gacgtacacg tctacgtcaa cagaacggcg gcgcatgagg gcacgaacct
     4741 cgaagacatc gaagaggcct tgaggcagct ggactacgtc tcccacgtcg aggccaaccc
     4801 ggtgggtaac gtggtcgccg tctccttcga gggaggcaag accgagcagg agggcatcaa
     4861 ggatgccgtc gaaagtatcg gctacgaggt ctcgaggttc tcgaaaagat ccaccatcgc
     4921 cgaccaggac cgaaacctgt gggatatata gctgtcagcc atgtagcaat tagctatcag
     4981 ctatgagcca gaacagaagt cgaagctaga gcggttctcg aaaaggcttc aggctacagg
     5041 tttcgggctt cagaagaatt gcctgaggcc tgtagccttt tctcgggttc actctgtccg
     5101 cacatcgtcc caaaagctga cagctgattg ctcgccaagg ggttgggccc cctggcttcc
     5161 gctctaggcg tctgccccct tggcgataag cagcagtacc acgcgccagt ggcatgatgc
     5221 tgtggcgggg tctgctgcga ctttctctcg caggttgtcg aagaaggcgt cggctatttg
     5281 ctcgtgttcc tggggggtgc ggtccgcgtc gagggccccg aagagcgagg gtccgtaggc
     5341 cgcccggaag aacgcttcgt agtcggcggc gaaggctctg gcgtcaccgc tctgctcgta
     5401 ctcgggccag aacgggtcag gcaggacgac ttcggaggat gattcgagct caagggggct
     5461 gcctttctgg cctggggaga acggggtttc gaactctttc agcgtgcggt tgtaggtcgg
     5521 gatgaccatt cgctcgtact cgtcggggcg gagcgtgtcg ccttcgacta tctcctgcaa
     5581 tgccgcgttc gccatgtcca tgaggccctc ggctccgctg gcaccctcgt cgtcgctcgc
     5641 cccgccaagc acgacgaggc ggcctccggg ccgaagctcg tggctcctgt ggtacaggaa
     5701 ggcctgccag tccccttccg attgcctggc gaacgcttcc ttcaccgtgc cggccgcccg
     5761 gttggaccag atgtggtcgg ggatggtcgc agggacgcgg ctgagccagt ggatcgcgat
     5821 ggcgttccac ccgatgtgaa ggtgggagtc ggggaagagc cgctcgtaga acgatttgcc
     5881 tcccgcgtat gcgaacacgt tggaggttcc ctgcaggtag cttgccgggg aggccagcaa
     5941 cgtaaacaac gggtcgaaat cgttggtggg caggtcagtg tggaagacgg agacggcgag
     6001 gtcctccgac gagcgttgcc tgaccccgtc gatgatggtc cgcatcggct ccatagagtt
     6061 gtttcccccg gccacgccgt aatccgcgac ctgcaacacc cctccggcat cggggagcgg
     6121 gacggccccg acggcgtgct ccagcaaggg tagcccgaac gatacggctc cgcgctgcgg
     6181 cctcgaatgc ctgttgtaga aacccttgcc ctccatcacg cccaggtctc cggacgcgcc
     6241 agcggtcttc ttctcggtca tcttctgttt ccccccatag tagatgcgtg actactgctc
     6301 tcggtctccc cgctcaccag gcgcggtctt gctcaccacc ccttcgagca gcacccggtt
     6361 ggggacgtag tggatgcgtc cgtccgccga tctcagcgtg accgaggcga ggcccagctc
     6421 ctccaccgtg ccctctatgc cgtttaccga gatcacatcc ccttccctga tgccgtttcc
     6481 ctgcacgtag cggcccgcgg cgatgttccc agagagaggg accaggccaa ggccaagggc
     6541 cagggcggcc acgatcccgg ccgcgccgaa gaggatcgcg gccaggagta ccaggatgga
     6601 gatattgatc cccagctgcg cggcggccag cagaaaggcg atcgcgagca caaaggcgaa
     6661 gaccgcgcgg ccgaacagat ccccacccct caggccagcg ctctcggatc gtatgatcgc
     6721 caggttggag agccaacccg ccgccgcgag gcctccggcc aggatcagga cagcgaccac
     6781 ggccttcccg gcgagtgcga ccacgagatc catgttgcgc tccagggatg ccaaccccag
     6841 gatgctcagg gccgcggcga cccccgcaag cagtaccccc cagaacagca cgtacgcgac
     6901 gacgaaagag gggcttctcc cgaagcccac ccgctgcaaa gaggccgccg cccccgtcga
     6961 ctcgaacagg cgatccagcc caacccgctg caataacctg gctcccagac gctgcagaag
     7021 gcgcgccacg aggaaggcga ccaccagcac caggacggcg ccgaccagcc tcggggtaaa
     7081 cccggaaata gctgtccaca aagcctccag ggcgttggcc atctgcaaac tcctccgcac
     7141 acgagacccc gcgctcctcc tgccggcgcg ggcggcatac atactagctt acaccgtcct
     7201 caggttcatt ggacggccag cctcggcccg atctaacccc tccccaccct ccttgcaatc
     7261 cagggtgcgg atgctaggat ttctgatgct tccagggccc gtagctcagc cgggagagcg
     7321 ccgccctcgc atggcggagg tcacgagttc gagtctcgtc gggtccactc accgaaaccc
     7381 tcataactac gcggaaactc acgaaaaaga gaagggctag aatcgtctcc cggccctcac
     7441 tacaccagct aatacaccaa cgcggcccca atgccggcaa ctcctcctta tccgaaggca
     7501 tctcccattg cgccggatgc ctgatcccca tcgagggaag gtggtagtgc gagtaggtgt
     7561 cgagtgtcat ggcgatggtg gcgtgtccga ggagctcctg aacgatcttc gggtggacgc
     7621 cacggccgag tagcagatcc ctcgaacacc agggcctcat gaagtatcta gagcttgctt
     7681 ggggccatgt agttgcagat gtgctggg
//
LOCUS       DBNGVD010000229         4007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4419948, whole genome shotgun sequence.
ACCESSION   DBNGVD010000229 DBNGVD010000000
VERSION     DBNGVD010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4419948"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1157
                     /locus_tag="AC16CM_06435"
     CDS             336..1157
                     /locus_tag="AC16CM_06435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139501.1"
                     /translation="MERSAGSEDYVLRGGRQGANRLRILNRVKWPTTEPLLEAAGLRA
                     GMRCLDVGCGGGDVTLKMAAFVGGEGHVVGVDRDPSILQLARWEAEDRGLPVTFRTVE
                     AEELSEESAYDLVFSRYLLSHLPRPQRVLEAMVRAARPGGRLVLEDVYFPGHVCYPPS
                     AALDRFVELYQAVARTNEGGDAAIGVRLLGMALEAGLEEVRVGLVVPTFREGEGKLVT
                     QVTMEHIREAVVGAGLATSSEVDSIVDELGRLAADDRTLMSMAPTFQVWGRKAGR"
     gene            1317..1724
                     /locus_tag="AC16CM_06440"
     CDS             1317..1724
                     /locus_tag="AC16CM_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3139502.1"
                     /translation="MADYGASMLDHLNLGVSDIERSRTFYTAALGAIGIEELLSFGPQ
                     DTASSSKIIGYGVGAKPFFWLVDGQRVGENTHVAFAVDSRDGVHAFYEAALAAGGKDN
                     GAPGLRPEYHEDYYGAFVLDPDGINVEAVYHGA"
     gene            1953..3725
                     /locus_tag="AC16CM_06445"
     CDS             1953..3725
                     /locus_tag="AC16CM_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3139503.1"
                     /translation="MADQRFGRFGDPAEYVETDVLIVGAGAAGLRAAIELTRAGVDVL
                     VLGKRRHGDAHTVWAAGGINASLGNLDPGDRWDIHAADTVGEGHFVNDPRAVEILARE
                     APERIRELYDWGCPFSLTEDGNLNQRYFGAQSFRRTCFAGDRTGEAMMGTLVDRAREE
                     GVPYREDVYVTKLLKSGGRVNGAVAFDLSSGRRILFGARAVVLAAGGYTSIYRRGSSR
                     PEENNGDAMALAYGAGAALKDMEFVQFHPTGMVEPEEMDGRLVTEAVRGEGGRLYNAE
                     GGRFMEFYSPERMELDARDVVARANYREIQEGRGTPEDAVLLDISHRDPEYIKERLPN
                     MYEQFMEQGVDITEEPMKVSPTAHYGMGGIEVEPRTCATVVEGLYAVGESAAGVHGAN
                     RLGGNSLAETVVFGKILGEHLARTIDDLPGAPLDEEAARERHAYLDALATGAGTNDPA
                     ELIDELRGLVWDHAGIVRTAGGLREGLDRLRDLRKRAGDLGAPGRPSDRPGRWLEHAL
                     DLGAMIVAAEAVLRGALAREESRGAHHREDFPEESDAWRKNILCARDEDGEMYLWTEP
                     VAEVSPEVQKALDEDYSLDYHYLE"
BASE COUNT          704 a         1162 c         1461 g          680 t
ORIGIN      
        1 tgcacaacaa gcagagaacc ttactaggtc gtgaacgagt tacaaggcgc tacatgccgg
       61 ttttcagccc tgtttgtgaa ccaattacat tgaggttgcg aacatgttac ggatatgtta
      121 cggcgggagg tctgtcgcta gaaagtcctg ctaatgcggt tcatttagta tgttgtggat
      181 gatgaggtgt gggctgcacc gcatcatgaa aaaggggagt cttttcgcgt tcggcgattg
      241 gtggtgaaaa ttcccgatga tgcgctccga gttgcaggat ggcattatgt ttgcggggta
      301 cggcgtacgg tggcagcggg cggaagggtg ccgggatgga acgttcggcg ggttcagagg
      361 attatgtgct tcggggtggc cggcagggag cgaaccgact tcgcatattg aatcgggtca
      421 aatggccgac aacggagccg ctgctcgagg cggctggttt gcgggccggg atgaggtgcc
      481 tggacgtggg gtgcgggggt ggggacgtga cgctgaagat ggccgccttc gtcgggggtg
      541 agggccacgt ggtcggcgtc gatcgcgacc cgtcgatcct gcagctggca cgctgggagg
      601 ccgaggatag gggcctgcct gtgaccttcc ggacggtgga ggccgaggag ttgtccgagg
      661 agtcggccta cgacctggtc ttctcccgct atctcctctc gcacctgccc cggccgcagc
      721 gggtcctgga ggcgatggta agggccgcgc ggccgggcgg tcgcctggtc cttgaagacg
      781 tctacttccc cggtcacgtc tgctacccgc caagcgccgc cctcgaccgc ttcgtggagc
      841 tctaccaggc cgtcgccagg acgaacgagg gtggggacgc ggcgatcggg gtgcgcctgc
      901 tcgggatggc gctcgaggcc gggctggagg aggtacgcgt cgggctcgtc gtgcccacct
      961 tccgcgaggg cgagggcaag ctggtgacgc aggtcacgat ggagcacata agggaagccg
     1021 tggtcggggc agggttggca accagctccg aggtggacag catcgtagac gagcttggca
     1081 ggcttgccgc agacgacagg acgctgatga gcatggcgcc cacgttccag gtgtggggcc
     1141 gcaaagccgg gaggtgagga ccgtccgggt cggccggtac cgtgccgtct tggtagccct
     1201 gaggccctag ccttgggtcc ccgaaactgg gcggtgtacc cggccgtagg gtccgctatg
     1261 cttgggccgg tttctggtgt tcgatccctc gcctggagag aaaggagccg gtctagatgg
     1321 cagattacgg cgcttcgatg ctggatcacc tcaacctcgg ggtaagcgac atagagcggt
     1381 cgcgcacctt ctatacggcg gcgctcggcg ccatcgggat cgaagagttg ctcagcttcg
     1441 gtccacagga caccgcttcg agcagcaaga taatcggtta cggcgtgggg gcgaagccgt
     1501 tcttctggct cgtggatggt cagcgcgtgg gggagaacac gcacgtggcc ttcgccgtcg
     1561 attcccgcga cggtgtgcac gcgttctacg aggcggcgct tgccgcgggc ggcaaggaca
     1621 acggcgctcc cggactacgt cccgagtatc acgaggacta ctacggcgct ttcgttctgg
     1681 atcccgacgg gataaacgtg gaagctgtct accacggtgc ctgagggctc ggttcgctcg
     1741 acccgtagag cattgcccct gcgggcttgg gaaccgccac gtagttgggt accttcgagg
     1801 tagtccttcg agcgaagtct ttcacaaacc ttcgacgggg aagttaatcg ggtcacctta
     1861 gcgcgacgtc ggggcgcgta tgctctacgt tacaaaacaa acgcgccccg ggtgcatcgg
     1921 ggcggggacg atcgacgtga gaggcgataa gcatggcaga ccagaggttc gggcgtttcg
     1981 gcgaccctgc agagtacgtg gagactgatg tattgatcgt gggcgccggc gcggcggggc
     2041 ttcgcgcggc catagagctg acgcgggccg gcgtggacgt gctggtgttg ggcaagcgcc
     2101 gtcacggcga cgcccacacc gtatgggcgg ccggcggcat caacgcctcc ctcggcaact
     2161 tggaccctgg cgatcgctgg gacatccacg ccgccgacac cgttggcgag ggccacttcg
     2221 tgaacgatcc aagggccgtc gagatcctgg cgcgagaggc accggagcgc atacgggagc
     2281 tctacgattg ggggtgcccg ttctccctga cggaggatgg caacctcaac cagcgctact
     2341 tcggtgccca gtccttccgg cgaacctgct ttgcggggga tagaacaggc gaggcaatga
     2401 tgggcacgct cgtggacagg gccagggaag agggcgttcc ataccgcgag gatgtctacg
     2461 tcacgaagct cttgaagagc ggggggcggg tcaacggggc cgtcgctttc gacctctcaa
     2521 gcgggcgaag gattcttttc ggggcacggg cggtcgtgct ggccgcgggt gggtacactt
     2581 ccatctaccg gcgcgggtca tctcgtcccg aggagaacaa cggggacgcc atggcgctcg
     2641 cctacggggc cggggcggcc ctcaaggaca tggagttcgt gcagtttcac ccgaccggta
     2701 tggtggagcc cgaggagatg gatggcaggc tcgtaaccga ggccgtcagg ggtgagggag
     2761 ggcgtctcta caacgccgag ggcgggcggt tcatggagtt ctactcgccg gagcgcatgg
     2821 agctcgacgc ccgcgatgtg gtggcgcgcg ccaactaccg cgagatacag gagggacgcg
     2881 gcacccccga agacgccgtg ctgctggaca tctcccatcg cgaccccgag tacataaaag
     2941 agcggctacc gaacatgtat gagcagttca tggagcaggg cgtggacata accgaggagc
     3001 ccatgaaggt ctctccgacc gcccactacg gtatgggcgg tatcgaggtc gagccgcgga
     3061 cctgcgccac ggtcgtggag ggcctgtacg ccgtcggcga gtcggccgcg ggcgtgcacg
     3121 gcgccaacag gctcgggggc aactcgctgg ccgagaccgt cgtcttcggc aagatcctcg
     3181 gcgagcatct cgccaggacc atcgacgacc tgcccggtgc gccgctcgac gaggaggcgg
     3241 cacgcgagcg gcacgcgtac ctcgacgcgc tggcgaccgg ggccgggacc aacgacccag
     3301 cagagctcat cgacgagcta cgcgggctcg tctgggacca cgccggcatc gtccggacgg
     3361 ccggggggct gcgcgagggg ctcgaccggc tccgggacct ccggaagcgg gctggggacc
     3421 tcggggctcc cggaagaccg tcggaccggc caggtcgatg gctcgagcac gccctcgacc
     3481 tcggtgccat gatcgtcgcg gccgaggctg tccttcgcgg cgccctcgcc agggaggagt
     3541 cgcggggcgc ccaccaccgc gaggactttc ccgaagagtc ggacgcgtgg cgcaagaaca
     3601 tcctctgcgc cagggatgag gatggtgaga tgtacctgtg gaccgagccc gtggccgagg
     3661 tgtcgccgga ggtgcagaag gccctcgacg aggactacag cctcgactat cactacctcg
     3721 agtaggtccc gtcacgtgat agtgagcccg cgtgccatcg tggttcgggt tatagaatgc
     3781 cgggtaaaat cagggggccg gggatggatc ccggccagcc ctgtataacg agacccggcg
     3841 aagcgtccgc tagcgaggga aagctcagct cgtgagcgac caccacatga tagaagaggg
     3901 cgaacaggag gccgccatcg gggctttcct gctggcccac gagggccata ccggggacgt
     3961 ggaggtccag ataggagcgt ggaccatcta ctgccggtgc gccctct
//
LOCUS       DBNGVD010000230         8482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4419965, whole genome shotgun sequence.
ACCESSION   DBNGVD010000230 DBNGVD010000000
VERSION     DBNGVD010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4419965"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="AC16CM_06450"
     CDS             <1..1319
                     /locus_tag="AC16CM_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HIR3139504.1"
                     /translation="GDVETLGRIEELEAEANAVYGNHRSVVGGEKLVENEVRELLRTS
                     EDEGIRREAWEASKSVGRAVEGTVRELARLRNRLAREQGYEDYYARSLELQEIQAEEL
                     AGIMEDLAFATDAPFRRLKDDLDVELRKKFGVEEVMPWHLSGPFFHRASDLAGIDLDR
                     FFEGKDLEELTRKTFDGLGLDVRGVISRSDLYEREGKSQHAFCTRIGREYPYDVRVLA
                     NVRPDANWMDAMLHEFGHAVYDRHINPRLPYLLRSFAHANTTEAIALMMGALVDDPGW
                     LRSIAGADAGDLDADAEKLGVHRRVGRMILTRFVLVMYHFEQALYADPDSEDLNRVWW
                     DLVGRYFYVDRPPGRDEPDWAAVIHVAVAPVYYHNYMLGELISAQLRNYLESHITRGP
                     FYDNEVAGRYLLESFFGPGARENWRDTVQRATGEPLNAQHFLEALR"
     gene            complement(1534..2484)
                     /locus_tag="AC16CM_06455"
     CDS             complement(1534..2484)
                     /locus_tag="AC16CM_06455"
                     /inference="COORDINATES: protein motif:HMM:NF022114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HIR3139505.1"
                     /translation="MSITGRVLGRVLALQLLSLLVVTLISGCSGSDLTSRSEKVSGDA
                     ARGDVPESTVGASLIDGDLTRASDVSPQVVFVGRPEDGGGSGYDADTVLGVRYGLHHH
                     YERAVIDLGTGKRPAGTVPEWTLTSPEGEGLLRVNLPSAVATCVSGGEFDKGLLESFH
                     VVRAPDGGMFVDFLTRQPFRYRVLQLQDPARLVLDFEPSEESLKAPPPAQGGDTVLVE
                     PRPNVEVSDPLTVSGYSRNPEAANTITLTGPDGEILARRNVRSNDWSHTWGYFDATID
                     LPPFSGKGTLKVGTGNARDGTFEGVEVPVRAGHRPAARSG"
     gene            2660..3619
                     /locus_tag="AC16CM_06460"
     CDS             2660..3619
                     /locus_tag="AC16CM_06460"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3139506.1"
                     /translation="MIGLRDIERAQERLRGVALRTPLLPCPGGEEGRALYFKPENLQP
                     TGAFKLRGAYNKISSLSPEERGRGVVAHSSGNHAQAVAYAARALDTRAVIVMPRGAPG
                     VKLDATASLGAEIVLVGPDSAERSRKAEELAAEHGYVPVPPYDDEVLMAGQGTVGAEI
                     LEDLPDAESVLVPVSGGGLIGGVSAAIKLTRPGVRVIGVEPELASDARESLKSGTLVE
                     FPANRVARTIADGLRVQKLGEAPFEHIRAFVDDIITVKEEEILEAMRRLALRARLVAE
                     PSGAVTFAAHLFHREELPASLLTVAVISGGNVDPALLGAVVSG"
     gene            complement(3679..4461)
                     /locus_tag="AC16CM_06465"
     CDS             complement(3679..4461)
                     /locus_tag="AC16CM_06465"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3139507.1"
                     /translation="MYVYPIKSCGGISTTSAEVGATGLLYDRSWMLVDQTGEFMSQRA
                     HPRMALISTRLTDELLIVSAPGMPDLPIPLRQENGNLIDVEVWGDVNRGALVGGEADR
                     WFGEFLRFPCRLVRKPEDDPRPVDSVYAQSGDQTSFADGFAFLLVSEASLDDLNSRLE
                     APLPMNRFRPNFVVSGCGAYAEDEWDRLHIGGIPFRVAEACPRCAITTTDQETAERGK
                     EPLRTLATYRKIDGEVFFGRNLIHDAPGTVRVGDTLEITPRL"
     gene            complement(4550..5041)
                     /locus_tag="AC16CM_06470"
     CDS             complement(4550..5041)
                     /locus_tag="AC16CM_06470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139508.1"
                     /translation="MASSESLREFRTSDAREIARWPDSLEEVRRWAGSDPGWPVDVSV
                     FGRWHADPEVRPFIMLEGQEPVGYGEVWVDEPEREVELARIIVSPETRGRGVGRRLVC
                     LLLDQASLTGLPDAFVRVVPENAAAIGCYRGAGFSPVSETEREEFNRGQPVDYVWMHH
                     PLG"
     gene            complement(5044..6765)
                     /locus_tag="AC16CM_06475"
     CDS             complement(5044..6765)
                     /locus_tag="AC16CM_06475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3139509.1"
                     /translation="MSRMFGRTLREVPSGVEVEGHGLLLRAGFVRQLAAGVFSYLPLA
                     RRSIAKIEGILREEMDAVGGQEVSMPVVQPAEVWKRSGRYDSTGPELVRLRDRRDREL
                     VLAMTHEEIVAGLAAGEVDSYQRLPRLVYQIQTKFRDDPRPRAGLIRAREFTMKDAYS
                     LDRDEEGLDEQYQTLHEAYFRIFARCGLPAVDVGADVGIMGGSMSHEFMYLSPVGEDT
                     ILLCDACGYRANRQVAGFRKPLPEAEEQRPLEKIPTPNADTIESLAGYLEVPESRTAK
                     AVFMVGTIGNSERFVFAVVRGDMGLSETKLANAVGASELRPALEAEIRAVGAEPGYGS
                     PLGVEGAIVVADDAIVDSPNLVAGANEVGFHFVNVNYGRDFEADVVADLASAEDGSPC
                     PECGAAMRAERGVEVGNIFKLGTRYSEAFGASFLDAGGERKPIIMGSYGIGVGRLLAC
                     VAEEHRDEDGLVWPVSIAPYQVHLVVAEAGEPADGLYEELGSTGVEVLYDDRRESLGA
                     KFKDADLIGIPLRLTLTPRSLQRGGVEVKGRADKESHIVPVDDVVETVRSMLDDLKRG
                     AERAEGG"
     gene            complement(6793..7695)
                     /locus_tag="AC16CM_06480"
     CDS             complement(6793..7695)
                     /locus_tag="AC16CM_06480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139510.1"
                     /translation="MPSNLTGTHQDFRVRLMHDADLDRVLELRSIVRWSADPRAFDLL
                     RGVRGARWAVAEAQDGSLTGMVGAVPLGGVGIICHLAVHDEHRGSGLGALLSYWAVTY
                     LKSRGTRTIRLYSTQQAEGLYRSIGFHKIAPRTVYWLGQARQTSVSGWSGGHAVETLT
                     FGDLPELYGVDYWSCGAERSALILATLKLHPGQGLVSRDAAGRIKGYLIRSSLGRTVR
                     IGPFLASTPEVARLLLASALREAGGAPVRLTAPGPVDHHAHSLLLEFGFQGTQDRMRM
                     ELGETEAPPKDGLIHYGTTAYLAT"
     gene            complement(7971..>8482)
                     /locus_tag="AC16CM_06485"
     CDS             complement(7971..>8482)
                     /locus_tag="AC16CM_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3139511.1"
                     /translation="WTADQISDALRIAGEMNLDRLVSNQPQYNMIQRRIEPDVIPLCE
                     REGVGQVVFSPLAQGVLTGKYRPGDAPEEGTRAADPENNRFMQQLMNEEVLSAVDGLR
                     SVASDSGLSLPQLALAWVLRQEDVSSAIIGASRPEQVDDNAAASGVKLSADTLSEIDR
                     ILDGVTRFE"
BASE COUNT         1467 a         2802 c         2811 g         1402 t
ORIGIN      
        1 agggtgacgt ggagaccctt ggacgaatcg aggagctgga ggccgaggca aacgctgtct
       61 acggcaacca caggagcgtg gtcggcggcg agaagctggt cgagaacgag gtcagggagc
      121 tgctgcgtac ctctgaagac gaaggcatac gcagggaggc ctgggaggcc tccaagtccg
      181 tcggccgcgc ggtcgagggc accgtaaggg agctggcccg gctcaggaac cggcttgccc
      241 gcgagcaggg gtacgaggac tactacgccc gctcgctgga gctacaggag atacaggccg
      301 aagagctggc cgggatcatg gaggatctcg ccttcgcgac cgacgcgccc ttcaggaggc
      361 tgaaggacga cctcgacgtg gagctgcgaa aaaaattcgg cgtcgaagag gtcatgccct
      421 ggcacctctc cggtccgttc ttccaccggg catccgacct agccggaatc gacctcgacc
      481 gctttttcga ggggaaggac ctcgaggagc tgaccagaaa gaccttcgac ggccttggtc
      541 tcgacgtgcg gggtgtcata tccaggagcg acctctacga gagggagggc aagagccagc
      601 acgccttctg tacccgtatc gggcgtgagt acccctacga cgtgcgcgtg cttgccaacg
      661 tcaggccaga cgccaactgg atggacgcca tgctccacga gttcggccac gccgtctacg
      721 acaggcacat caaccccagg ctcccctacc tgctccgcag cttcgcccac gccaacacca
      781 ccgaggcaat cgccctcatg atgggcgccc ttgtagatga cccggggtgg ctgcgctcca
      841 tcgccggtgc cgacgcgggg gatctggatg cggacgccga gaagctgggg gtgcaccgcc
      901 gggtgggcag gatgatcctc acccgtttcg tgctcgtcat gtaccacttc gagcaggcgc
      961 tctacgccga cccggatagc gaggacctga accgcgtgtg gtgggacctc gttgggaggt
     1021 acttctacgt ggaccgcccg ccggggcgtg acgagcccga ctgggccgcg gtcatccacg
     1081 tcgccgtcgc ccccgtctac taccacaact acatgctggg ggagcttatc tcagcccaac
     1141 tcagaaacta cctggaatcc cacataaccc gggggccctt ctacgacaac gaggtagcag
     1201 gccgctacct cctggagtcc ttcttcggtc ccggtgcccg cgagaactgg cgagacaccg
     1261 tgcagcgcgc caccggagaa ccgctcaacg cgcagcactt cctggaagcg ctgcgctagc
     1321 acgctccggc ttcgcctcgc tttcggccag tcgtctcgat tcgcatcggt gggctcggta
     1381 accggggctt ccctccgcga cggtactcta tgcggaaggg cgaggcctca gccgttattg
     1441 gctgaaagcg accgaaggga gcgtgccgac aaacgaggcc gaaggccgag tgtgctgaca
     1501 aggcgcgaag cgccgtgctg acaagcgtag gcgttagccg gagcgtgcgg cgggccgatg
     1561 gcccgcccgg acggggacct cgacgccttc gaaggtgccg tcgcgggcgt ttccggttcc
     1621 gaccttcagg gttcctttgc cggaaaacgg ggggaggtcg atggtggcgt cgaagtaacc
     1681 ccaggtgtgg ctccagtcgt tgctcctcac gttcctgcgg gcgaggattt ccccgtcggg
     1741 gccggtcagg gtgatggtgt tggccgcctc tgggttacgg gagtagccgc tcacggtgag
     1801 ggggtctgat acctcgacgt tcgggcgggg ctctaccagg acggtgtcgc cgccttgcgc
     1861 cgggggcggt gccttcaggc tctcctccga cggctcgaag tcgaggacca gccgggccgg
     1921 gtcttgaagc tggagcaccc tgtagcggaa cggttggcgc gtgaggaaat caacgaacat
     1981 gccgccgtct ggcgcgcgca ccacgtggaa gctttcgagc aagcctttgt cgaactcgcc
     2041 accggagacg cacgtcgcta ccgccgaggg caggttaacg cgaagtaggc cttcgccctc
     2101 ggggctcgtc agggtccact cgggaacggt gccggcgggc cgcttgccgg taccgaggtc
     2161 gatgaccgcc cgctcgtagt ggtggtgaag cccgtaacgc accccgagca ccgtgtcggc
     2221 gtcgtagcca gaaccgccgc cgtcctcagg acgtccgacg aacacgacct ggggggagac
     2281 gtcggatgcc ctggtcaggt ccccgtcgat aagagaagcc cccacggtgg actctggaac
     2341 gtcgcctcgg gcagcgtctc cggacacctt ctcagatcgg gacgtcaggt ccgatccaga
     2401 gcaaccagag attagcgtga ccaccagaag cgagagcaac tggagggcga gaacccgccc
     2461 caacacccgc ccggttatgc tcacgccccc gtgtccatgt acaggtattc ggtcataggc
     2521 gccggctatt gtagggtaat acggtttgca aggtgcttaa cgtggtgttg cgctcctgtt
     2581 acgatgcgat tcgcttcgcg aaccacgctg tcagctatca gcttttgaac cggaggctga
     2641 aagaaaggag ggacgcgtct tgatcgggct ccgcgacata gagagggcgc aggagaggct
     2701 gcggggggtg gcgctccgca cgcccctctt gccctgcccg ggcggtgagg agggcagggc
     2761 gctgtacttc aagccggaga atttgcagcc gaccggcgcc ttcaagctgc gcggggccta
     2821 caacaagatc tcctcgctct cacccgagga gcgcggccgt ggggtcgtgg cccattcaag
     2881 cggcaaccat gcacaggccg tagcctacgc tgccagggcc ctcgacacgc gggccgtcat
     2941 cgtcatgcca cgcggggcgc caggggtcaa gctagacgcc accgcctctc tgggcgccga
     3001 gatcgtgctc gtggggccgg atagcgccga gcgttcacgc aaggccgaag aactcgcagc
     3061 cgagcacggc tacgtccccg tgccccccta cgacgacgag gtactcatgg cgggccaggg
     3121 caccgtcggg gcggagatac tggaagacct gccagacgcg gagagcgtgc tcgtgcccgt
     3181 cagcggcggc gggctgatag gcggggtctc cgcggcgatc aagctcaccc gtcccggggt
     3241 gcgggtgatc ggggttgagc ccgagctcgc atccgacgcc agggagagcc tgaagagcgg
     3301 gacgctggtc gagttccctg ccaaccgggt cgcccgcacc atcgccgacg ggctgcgcgt
     3361 gcagaagctg ggcgaggctc ccttcgagca catccgtgcc ttcgtcgacg acatcatcac
     3421 ggtgaaggag gaagagatac tggaagctat gcgacgcctg gcgctgaggg cgaggctagt
     3481 cgcggagccg agcggggccg taacgttcgc cgcccacctc ttccaccgcg aggagctacc
     3541 agcctccctc ctgaccgtcg ccgtcatcag cggcggcaac gtggatccgg ccctgctagg
     3601 ggcggtggtc tccggttaga tcgagctatc agcggtcagc aagaaaagct gaaagctgac
     3661 ggctgatggc tgaccgctct aaaggcgtgg tgtgatctcg agggtgtctc cgacgcgcac
     3721 ggtgccgggg gcgtcgtgga tgaggttgcg tccgaaaaag acttcgccgt cgatcttgcg
     3781 gtaggtggcg agggtgcgta ggggctcctt gccacgctct gccgtctcct ggtccgtcgt
     3841 ggttatggcg cagcgtgggc aggcttcggc gacccggaag gggatacccc cgatgtggag
     3901 ccggtcccat tcgtcttcgg cgtaggcgcc gcagccgctg accacgaagt tgggcctgaa
     3961 gcggttcatg gggagggggg cttccagcct gctgttcagg tcatcgaggg aggcctcgga
     4021 gacgagcaga aacgcgaagc cgtcggcgaa gctcgtctgg tctccgctct gcgcgtagac
     4081 ggaatcaacc gggcgtgggt cgtcctccgg cttgcggacg aggcggcacg gaaaccgcag
     4141 gaactcgccg aaccagcggt ctgcctcccc accaacgagc gcgcccctgt tcacatcgcc
     4201 ccagacctcg acgtctatca ggttcccgtt ctcctgccgg agcggtatcg gcaggtcggg
     4261 catgcccggc gcgctgacga tcagaagctc gtcggtgaga cgggtggaga tcagggccat
     4321 cctgggatgg gcacgctggg acatgaactc gcccgtctgg tcgacgagca tccagctacg
     4381 gtcgtagaga agcccggtcg cgccaacttc ggcgctggtc gtcgagatac cgccgcacga
     4441 tttgatcggg tagacgtaca acccacttag agcaacgttc ccggcatagc gcaaagcatc
     4501 cctcccctgt tcggaaccgt ctccatgatc caacgtaacg ctggaggctt caacccagag
     4561 ggtggtgcat ccagacgtag tcgaccggct ggccgcggtt gaactcctcg cgctcggtct
     4621 cggacacggg cgagaacccc gcgccgcggt aacagccgat ggcggccgcg ttctcgggga
     4681 ccacgcgcac gaaggcgtcg gggagcccgg taagggaggc ctggtccagg agcaggcaca
     4741 ccagccggcg gccaacacca cgccccctcg tttccgggct gacgatgatg cgggcgagct
     4801 cgacttccct ttcgggctcg tctacccaca cctcgccgta gccgaccggc tcttgccctt
     4861 cgagcatgat aaacggtctg acttccgggt cggcgtgcca gcggccgaac accgacacgt
     4921 cgacgggcca tccggggtcg ctgccagccc accgccgcac ctcctctagc gagtcaggcc
     4981 agcgcgcgat ctcccgcgcg tctgatgttc gaaactcgcg aagtgactcg gagctggcca
     5041 cgcctacccg ccctcggccc tctcggcacc gcgtttcagg tcgtccagca tgctcctgac
     5101 ggtctcgacc acgtcatcga cggggacgat gtggctctcc ttgtctgcgc ggcccttgac
     5161 ctctactcct cctctttgca gggaacgggg cgtcagggtc agccgcaggg ggatgccgat
     5221 caggtcagcg tccttgaact tggccccgag gctctcgcgc cggtcatcgt acaacacctc
     5281 gacgccggtg gacccaagct cctcgtagag cccgtctgcc ggctcccccg cctcggccac
     5341 gaccaggtga acctggtagg gtgcgatgga gacgggccag acgaggccgt cctcgtcgcg
     5401 gtgctcctcg gcgacacagg ccaggagccg acctaccccg atcccgtatg accccattat
     5461 gattggcttg cgctccccgc cggcgtcgag gaagctcgca ccaaaggcct cactgtagcg
     5521 cgtacccagc ttgaagatgt tccccacctc gacgccccgc tccgcgcgca tggcggcgcc
     5581 gcattcgggg cagggcgagc cgtcctcggc cgaggcaagg tcggccacca cgtccgcctc
     5641 gaagtccctg ccgtagttga cgttcacgaa gtgaaagcca acctcgttgg cgccggccac
     5701 gaggttcggg gagtctacga tagcgtcgtc ggcgacgacg atggctccct ccaccccgag
     5761 cggagatccg tacccaggct cggcgccgac ggccctgatc tccgcctcga gcgcgggcct
     5821 gagttcgctg gccccaacgg cgttggccag cttggtctcg ctcagcccca tgtcgccacg
     5881 cacgacggcg aagacgaacc gctcggagtt cccgatggtg ccgaccataa agacagcctt
     5941 ggccgtccgg gactcgggaa cctccagata acccgccagt gactcgatgg tgtccgcgtt
     6001 cggcgttggt atcttctcaa gggggcgctg ctcctcggcc tccggtagcg gcttccggaa
     6061 accggctacc tggcggttcg cccggtagcc gcaggcgtcg cagaggagga tggtgtcctc
     6121 cccgaccggg gacaggtaca tgaactcgtg ggacatgctc ccacccatga tcccgacgtc
     6181 cgcgccgacg tcgacggccg gaaggccgca gcgggcgaag atcctgaagt acgcctcgtg
     6241 aagcgtctgg tactgctcgt ccaggccctc ttcatcccta tccaggctgt aggcatcctt
     6301 catggtgaac tcgcgggccc ggatcaggcc agccctcgga cgcgggtcgt cgcgaaactt
     6361 ggtctggatc tggtagacga gccggggcag cctctggtaa gagtcgacct caccggccgc
     6421 gagcccggcc acgatctcct cgtgggtcat cgcaagcacc agctcccgat ccctgcggtc
     6481 gcgcagcctg accagctcgg gccccgtcga gtcgtaccgc cccgacctct tccaaacctc
     6541 ggcaggctgc accacgggca ttgaaacctc ctgcccgccg acagcatcca tctcctcccg
     6601 caagatgccc tctatctttg cgatggatct ccttgccaga ggcagataag agaacacccc
     6661 ggcagccagc tgcctgacga acccggcccg gagcaacaac ccgtgtccct cgacctccac
     6721 accggaaggc acctcgcgta acgtccggcc gaacatcctg ctcatgcgca tgatttagcc
     6781 gtcagctctc agctaagtag ctagatacgc cgtcgtgccg tagtggatca ggccatcctt
     6841 cgggggcgct tcggtctcgc ccagctccat ccgcatccgg tcctgcgttc cctggaaacc
     6901 gaactccaga aggagggagt gggcgtgatg gtcgacggga ccgggggccg tgaggcggac
     6961 cggcgcgcca ccggcctctc gcaaggcgct cgcgagcagg aggcgtgcca cctctggggt
     7021 cgaggcaagg aaggggccga tccggaccgt gcggcccagg gagctccgga tcaggtaccc
     7081 cttgatcctg ccagccgcgt cgcgggagac gagcccctga ccgggatgga gcttgagggt
     7141 ggcgaggatc agggccgacc tctcggcccc acagctccag taatcgaccc cgtagagctc
     7201 gggaagatcc ccgaaggtga gcgtctcgac agcgtggcca cccgaccacc cggacacgct
     7261 ggtctgcctc gcctgtccaa gccagtacac ggtgcgcggc gcgatcttgt ggaaaccgat
     7321 ggagcggtag agaccttctg cctgctgtgt cgagtagagc ctgatggtac gtgtgccacg
     7381 gctcttcaga taggtcacgg cccagtagga gagcagggcg ccgaggccgg aacccctgtg
     7441 ctcgtcgtga acggcgaggt ggcagatgat gccgactccc ccgagcggaa cggcaccgac
     7501 cataccggtc agggagccgt cctgcgcctc cgccacggcc cagcgggcac cgcgcacccc
     7561 ccgcagcaaa tcgaaggccc tggggtcagc cgaccatctt acgatgctcc tcaactcgag
     7621 gacgcggtcg agatctgcgt cgtgcatgag gcggacacga aagtcctgat gggttccggt
     7681 caggttgctc ggcacgttac ggagggtagc tttctctact gcggtgtctt ctctcgtggt
     7741 gtaggctagc atttgtatcc ccgatccatg ttgactcctg tatggcgtct attccgcgcg
     7801 ggaggtccga gtgcgcgaac ctcagatgac gcggatgccc cgagcgcgac ccctagaggg
     7861 ccggtcgggg cggggacggt cgaggaggcc gtccgacgag agagctcggg gtaaaggtca
     7921 tgctatccac gcccaccatg ctacaccaga tggcacccgg cgggaagcgt ctactcgaac
     7981 cgggtaacgc catccagaat acggtcgatc tcggagagcg tgtccgctga gagtttcacg
     8041 ccggaggccg cggcgttgtc atcgacctgc tcgggcctgg aggccccgat gatggcgctg
     8101 ctcacgtcct cctggcgcag cacccacgcc agcgcgagct gcggcaagct caggccggag
     8161 tcggaggcga cggagcgcag cccgtcgacc gcggagagga cctcctcgtt catgagctgc
     8221 tgcatgaacc tgttgttctc cgggtcggcg gcgcgggtac cctcctcggg agcgtcgccc
     8281 ggcctgtact tgcccgtaag cacgccctgg gcgagcggcg agaacacaac ctgccctacg
     8341 ccctcgcgtt cgcacagggg tatcacgtct ggctcgatgc ggcgttggat catgttgtac
     8401 tgcggctggt tggagacgag ccggtccagg ttcatctccc cggcgatcct gagggcgtcg
     8461 gagatctggt cagccgtcca ct
//
LOCUS       DBNGVD010000231         2200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_959533, whole genome shotgun sequence.
ACCESSION   DBNGVD010000231 DBNGVD010000000
VERSION     DBNGVD010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_959533"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..847
                     /gene="map"
                     /locus_tag="AC16CM_06490"
     CDS             104..847
                     /gene="map"
                     /locus_tag="AC16CM_06490"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3139512.1"
                     /translation="MTIESDRDLAGLRRAGRVVALAIEEMKAALESGMTTAELDAVGA
                     AVYERHGARSAPQLVYGFPGVNLISVNDEAVHGVPGGRVIETGDLVKIDVTAEVGGYV
                     ADAAVTVAMPRGPRVHGELRDCAELAFARAASVARAGRPVNKIGRVVDNTVRGRGFVT
                     LAGLSGHGIGKTIHEPPTIPNRFDKKSDSLLTAGLVVTIEPIIAAGSDRTVEGSDGWT
                     LRTADGSPAAHHEHTIVITKGSPIVLTAA"
     gene            complement(849..1241)
                     /locus_tag="AC16CM_06495"
     CDS             complement(849..1241)
                     /locus_tag="AC16CM_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3139513.1"
                     /translation="MAGPETPKLMVDVVIPSENGVVLVRRGSEPFEGQWALPGGFVEV
                     GETVRQAAAREAEEETGLTVEVSRLVGVYSEPDRDPRGHNVSVAFLATVLSGRMRAAS
                     DAAEVEVLDPGSVELAFDHARIISDAMG"
     gene            1370..1978
                     /locus_tag="AC16CM_06500"
     CDS             1370..1978
                     /locus_tag="AC16CM_06500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139514.1"
                     /translation="MKLLCISDKVEAMLHGPNLTSYARGVDAVISCGDLPFAYLEYIV
                     TFLGVPVYYVLGNHDPGPEGPEYPGGCTPLDGRIVEAEGGLVLAGLSGSPMYSGGPNQ
                     YTERQMRGRARALSFRIRCRGLVGRPKPRILVTHSPPFGLGDAEDTAHVGFKSFVDFI
                     DRHEPPLLLHGHIHLYGPNQESVLKRGETKIINVYGHRIIEV"
BASE COUNT          393 a          711 c          730 g          366 t
ORIGIN      
        1 agtttacacc accatcagtg acccggaacc agacttcgtt cacagcacag gcctacaata
       61 cgaggatgcc catgccccca atgcccggac gagaggaaca gccatgacca tcgaatcgga
      121 cagggacctt gccgggctgc gtcgggcggg aagggtagtg gccctggcga tcgaagagat
      181 gaaggcggcc ctagagtccg gcatgaccac ggcggagctc gatgccgtgg gggccgcggt
      241 ctacgagcgc cacggggcga ggtcagcacc gcagttggtg tacgggttcc ccggcgtcaa
      301 cctcatcagc gtgaacgacg aggccgtgca cggtgttcct ggcggccggg taatagaaac
      361 gggcgacctc gtcaagatcg acgtcaccgc cgaggttgga gggtacgtcg cagacgccgc
      421 ggtcacggtc gccatgcctc gggggccgcg ggtgcacggg gagttgaggg actgcgcgga
      481 gctggcgttc gcccgagcgg cctccgtggc ccgggcgggg cggccggtga acaagatcgg
      541 ccgcgttgta gataacaccg tgcgcgggcg aggcttcgtg accctcgccg gtcttagcgg
      601 tcacgggatc ggcaagacca tccacgagcc tcccacgata cccaaccggt tcgacaagaa
      661 atctgactcg ttgctaaccg cggggcttgt cgttaccatc gagccgatca tcgccgccgg
      721 ctcggacaga accgtggagg gttccgacgg ctggacccta aggacggcag acggaagccc
      781 ggcggcgcac cacgaacaca ccatcgtaat cacgaaaggt agccccatcg tgctcacggc
      841 ggcgtgaact atcccatcgc gtcggagatg attcgggcgt ggtcgaaggc cagttcgacg
      901 gagccgggat ccaggacctc cacctccgcg gcgtcggagg cggcccgcat cctgccggag
      961 aggacggtgg ccaggaaggc gacgctgacg ttgtggccgc gcgggtcgcg gtccggttcg
     1021 gagtagacgc cgacgagacg cgatacctct acggtgagcc ccgtctcctc ctcggcctcg
     1081 cgtgccgccg cctgcctaac cgtctccccc acctcgacga agccccccgg caacgcccac
     1141 tggccctcga atggctcgct accgcgacgg accagaacga ccccgttctc ggatgggata
     1201 accacatcca ccatcagctt cggcgtctct ggtcctgcca aggttcgctc ctcccgcgtg
     1261 ttccaggatc tgtccgaggc cctgattata tgagatccgg gggtggggac ggtgtcgcgt
     1321 tctagggggg cgcatcggtt atgctagggg aactgatggg tggtcgctag tgaagttact
     1381 ctgtatcagc gacaaggtgg aggccatgct ccacggcccc aacctgacct cctacgcgag
     1441 gggcgtggac gcggtgatct cctgtggaga ccttcccttc gcctacctcg agtacatcgt
     1501 cacgtttctg ggcgttcccg tctactatgt gcttgggaac cacgaccccg gcccggaggg
     1561 gccggagtat cctggcggtt gcacgccgct tgacgggagg atcgtggagg cggagggtgg
     1621 cctggtgctc gccggtctct ctggctcgcc catgtactcg gggggaccca accagtacac
     1681 ggagcgccag atgcgcggcc gggccagggc gctctcgttt cggatacgtt gcaggggcct
     1741 cgtcggccgt ccgaagcccc ggatcctcgt cacccactcg ccgcccttcg gcctcggcga
     1801 tgcggaggac acggcccacg tcggcttcaa gtcgttcgtg gacttcatcg accgccacga
     1861 gccacccctc ttgcttcacg ggcacatcca cctctacggg ccgaaccagg aaagcgtgct
     1921 gaagcgggga gagacaaaga tcatcaacgt ctacggccac cgcatcatcg aggtgtaggg
     1981 agcaggtagc ccacagaagc agcgaggccg gtaaccgggt ctcgccccct tgtcggcggg
     2041 gccggggggc tgtagattga ccgtattatc atggaccctc acgaacaatc cgacagagat
     2101 ttttcacgcg cccgcaggag ggccttcctg cgcaaggtag gcgcgtacct gcgcggggat
     2161 tccggttcca accagttgct ctccttcgac gaggtcaagg
//
LOCUS       DBNGVD010000232         4007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4238919, whole genome shotgun sequence.
ACCESSION   DBNGVD010000232 DBNGVD010000000
VERSION     DBNGVD010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4238919"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="AC16CM_06505"
     CDS             complement(<1..288)
                     /locus_tag="AC16CM_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR3139515.1"
                     /translation="MAHLEAAEGALGSDQARRHLEQARRTARDNLIEARHLVAALRPE
                     ILEGSSLSGALERLARRWSEETDIPATVSVTGGESTLTQESQVALFRAAQEA"
     gene            complement(414..989)
                     /locus_tag="AC16CM_06510"
     CDS             complement(414..989)
                     /locus_tag="AC16CM_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139516.1"
                     /translation="MMKPFEDMLTERAASEPSTGDAARGTNAWIRWAVIWDALFYVLL
                     VVVLVSSLLDMGFYGRPQLVLGGLTVLFGLWYWFMIIRHRRWIQNDLPMLAYAAGAIA
                     LCVTLIWLNPTYNLLLFVMYSQLYSFLRIRWVVPASLLLTGLLVLRGVVAAPEAWRSW
                     VVIATLSVFFGIFFALWINSRSSSRAGSAGS"
     gene            complement(1030..1752)
                     /locus_tag="AC16CM_06515"
     CDS             complement(1030..1752)
                     /locus_tag="AC16CM_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139517.1"
                     /translation="MTVVETRLFLREPTGLFFTLLFPVMVLLLFSAIFGNGRVPGETG
                     LRMIDVMAPSYTGMVIGTTALLGLPITLAVYRQQGTLRRLRATPLHPSVVLVAHVLVN
                     LLMTAVGISLLLLTARIFYDLRLPEAPLSVVFAFLVASLSFFALGFVLAGVLPNARTA
                     QIVGQVVFFPMFFLSGAAGIQPDMFPEALRLVSDLLPLTYVVELVQGLWISGEWNLRA
                     LAVLLGVMVASVLVSARTFRWE"
     gene            complement(1782..2177)
                     /locus_tag="AC16CM_06520"
     CDS             complement(1782..2177)
                     /locus_tag="AC16CM_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DrrA family ABC transporter ATP-binding protein"
                     /protein_id="HIR3139518.1"
                     /translation="MRENRARGATVLLVTHSMEEAEKLCDRVAIIDHGRVVALDSPET
                     LVREHDPEASVRLEGVDGLDPAVLRRIDEVSRSEWDEDELVVYGRGPLLARVIVALDH
                     HGFTPRGVCVGRPSLEYVFVEKTGREVRD"
     gene            complement(2170..2685)
                     /locus_tag="AC16CM_06525"
     CDS             complement(2170..2685)
                     /locus_tag="AC16CM_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139519.1"
                     /translation="MEPVIEVMDLRKRYGKVVAIDVISFSVGRGEIFGIVGPNGAGNS
                     TTVECLARLRERDGGTVSVLGLDPHRDGRKLRERIGVQLQEAALPDDVKVWEALDLYA
                     SFYRRSAGWEGLLKQWGLAPKRDTRFENLSGGQKQRLFIALALINRPELVFLDEITTG
                     LDPQAHVGPGA"
     gene            2948..3853
                     /locus_tag="AC16CM_06530"
     CDS             2948..3853
                     /locus_tag="AC16CM_06530"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3139520.1"
                     /translation="MRLRNWRSKGESVTEQEILDKGYANPDALVSTQWVADNLDNTEN
                     YRIVESDEDVLLYDIGHVPNSVKIDWVEDLNDPLMRDYLDPEHFANLMAEKGIGPDTT
                     VIFYGDRNNWWATYALWVFRLFGHENVKVMDGGRVKWEAEGREMTQDVPSFPRAEYPV
                     PERDDSRIRAFKEDVDKHLDVSGPLVDVRSPGEYKGELLHMPDYPQEGALRGGHIPGA
                     ANVPWARAVREDGTFKSADELRGIYEGEAGLSDGEDVIAYCRIGERSSHTWFVLSYLL
                     GYDNVRNYDGSWTEWGNLVRAPIER"
BASE COUNT          806 a         1261 c         1283 g          657 t
ORIGIN      
        1 ggcctcctgg gcagcccgaa acagggcgac ctgcgattct tgcgtcagag tactctcacc
       61 gcctgtcacg gagactgtgg cggggatatc cgtctcctcg gaccagcgcc ttgccaggcg
      121 ctccagcgca ccagacaggg atgaaccctc caggatctcg ggccgcaagg ccgccacgag
      181 gtggcgggcc tcgatcaggt tatcgcgggc cgtgcgccgc gcctgctcga ggtgccgtct
      241 cgcctgatcc gagcccagcg ccccttccgc cgcctcgagg tgggccacga tgctgataaa
      301 cccctgggcg agggtgtcgt ggatctccct ggccagacgg ccacgctcct cgaggatgcc
      361 ggcgcggcgc tgtctggcgg cgagttgctc gcgggtagac tccagctctt ctatcagctc
      421 cctgcgctcc ctgctctgct cgatgatcga gagttgatcc agagcgcgaa gaagatcccg
      481 aagaagacgg agagcgtcgc tatgaccacc caggaacgcc aggcctctgg cgcggccaca
      541 acgccccgta agaccaggag gcccgtcaga agcagggagg caggaactac ccagcgtatc
      601 cttaggaagc tgtagagctg ggagtacatc acgaagagga gcaggttgta ggtcgggttc
      661 agccagatca gggtgacgca caacgctatg gcgcccgccg cgtaggcgag catggggagg
      721 tcgttctgga tccagcgccg atgccggatg atcatgaacc agtaccagag tccgaacaga
      781 acggtcagcc cgcccaacac gagctgcggc cttccgtaga agcccatgtc caggaggctg
      841 gagaccaaaa cgacgaccag caacacgtag aagagtgcgt cccagatcac ggcccacctg
      901 atccaggcgt tggtgcccct ggccgcgtcg ccggtcgagg gttccgacgc cgcacgctcc
      961 gtaagcatgt cctcgaacgg cttcatcatg ggtatcttac gcccgcatac ggtgtctggt
     1021 cggtgaccgt tactcccatc tgaaggtcct ggctgagacg agaaccgagg cgaccatcac
     1081 gccgaggagc accgcgagcg cccggaggtt ccattccccg gagatccata gcccctgtac
     1141 gagctccacg acgtaggtga gcggcagcaa gtcggagacg aggcgcaggg cttcggggaa
     1201 catatcgggc tgaatccccg cagccccgga gaggaaaaac attgggaaga agacgacctg
     1261 accaacgatc tgggctgttc tggcgttggg caggaccccg gcgagaacga accccagggc
     1321 gaagaagctc aggctggcca ccaggaaggc gaacaccacg gagagcggtg cttccggcag
     1381 tcgcagatcg tagaagatcc tggccgtaag gagcagaagc gagataccga cggccgtcat
     1441 gagcaggttg acgagcacat gcgccaccag taccacggac gggtgcaggg gcgtggcgcg
     1501 caggcggcgc agtgtgccct gctgcctgta aacggccagg gttatgggga ggcctagcag
     1561 cgcggtggtc ccgatgacca tacccgtgta ggagggggcc atgacgtcga tcatgcgcag
     1621 acccgtctcg ccgggaaccc tgccgttgcc gaagatggcg gagaagagca acaacaccat
     1681 gacggggaac aacagggtaa aaaagagccc cgtcggctcc cgcagaaaca gcctggtttc
     1741 cactacggtc atcttccaaa gagctcgcat cgtacctccc tctagtccct cacctcgcgc
     1801 ccggtctttt ctacgaacac gtactcgagg ctgggtcgtc ccacgcatac ccctctcggc
     1861 gtgaacccgt ggtggtccag cgccacgatg acgcgtgcca gaagcggacc acgaccgtag
     1921 actacgagct cgtcctcatc ccattccgac cggcttactt cgtctatccg gcgcaggacg
     1981 gcgggatcca acccgtccac tccttccagg cgtacgctgg cctccggatc gtgctctctg
     2041 acaagggttt cgggcgaatc gagggccacc actcggccgt ggtcgatgat cgccaccctg
     2101 tcgcagagct tctcggcctc ctccatggag tgcgtgacca gcagaaccgt ggccccgcgg
     2161 gcacggttct cacgcaccag gtcccacgtg ggcctgcgga tcgaggcccg tggtgatctc
     2221 gtccagaaac acgagctctg gtctgttgat gagcgcgagg gcgatgaaga gccgctgctt
     2281 ctggcccccg gagaggttct cgaacctggt gtcccgcttc ggggcgagac cccactgctt
     2341 cagcagcccc tcccagcccg cggaacgccg gtagaacgag gcgtagagat cgagagcctc
     2401 ccacaccttg acatcgtcgg gcagggcggc ctcctgcagt tgcaccccga tacgctcccg
     2461 gagctttcta ccgtccctgt gcgggtccag tccgagcacg ctgaccgtgc ctccgtccct
     2521 ctctcgtagt ctggccaggc actcgaccgt ggtagagttt ccggccccgt tgggaccgac
     2581 gatgccgaag atctcgcccc ggcccacgga aaaagagatc acgtctatgg ccacgacctt
     2641 gccgtatcgc ttgcgcagat ccataacctc gataacgggt tccacccgtt cacctccttc
     2701 tcgctctcct ggtaggagta tccgtccggc gaggaccgcc gtgatcggta gacgggccag
     2761 atgtggaagg aattcaggtt gatgcctctg tcagccgatc ggttgattgg cctgttgtat
     2821 ccgcgcgacg gagcagtatt tgctggctcg ggaaggtcca gcacggagat cgctcagagc
     2881 gtgttgtgat ggcgggggga gagcagaact ttaaatccga ccaactctat cggagttggg
     2941 ttagaatatg cgcttgcgta attggagatc gaaaggagaa agcgtgaccg agcaggagat
     3001 actggacaag ggctacgcaa accccgatgc cctggtcagc acccagtggg tggcggacaa
     3061 cctggataat accgagaact atcgcatcgt ggagtccgac gaggacgtgc tcctctacga
     3121 cataggccac gttccgaact ccgtaaagat agactgggtc gaggacctca acgacccact
     3181 gatgcgcgac tacctcgacc ccgagcactt cgcgaacctg atggccgaga agggcatcgg
     3241 cccggacacc acggtcatct tctacgggga taggaacaac tggtgggcca cctacgccct
     3301 ctgggtgttc cgtctcttcg gccacgagaa cgtcaaggtc atggacggcg gaagggtcaa
     3361 gtgggaggcc gagggccgcg agatgaccca ggacgtgcca tcctttccaa gggccgagta
     3421 ccccgtgcca gagagggacg attcgaggat acgggccttc aaggaggatg tggataagca
     3481 cctggacgtc agcgggccgc tcgttgacgt gaggagcccg ggcgagtaca agggcgagct
     3541 gctccacatg cccgactatc cccaggaggg cgccctcagg ggcgggcaca tccccggggc
     3601 cgcgaacgta ccgtgggcgc gggccgtcag ggaagacggg accttcaaga gcgccgacga
     3661 gctacgcggc atctacgagg gcgaggccgg cctcagcgat ggggaggacg taatagcgta
     3721 ctgcaggatc ggggagcgct cctcgcacac ctggttcgtg ctgtcctacc tcctcggcta
     3781 cgacaacgtg cgcaactacg acggctcgtg gaccgagtgg ggcaacctgg tcagggcccc
     3841 aatagagcgt taggaacgat ttggagccca ccctggaccg caagagccgc gtagctctgc
     3901 tcgtggaaca cgtcgagagc ccccgccaca agggggctct cgaaagcgcc gacgtcgtgg
     3961 tgccgggagg aagccccgag tgcggtggtt ctgtaaccgt ctacctc
//
LOCUS       DBNGVD010000233         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4603259, whole genome shotgun sequence.
ACCESSION   DBNGVD010000233 DBNGVD010000000
VERSION     DBNGVD010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4603259"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="AC16CM_06535"
     CDS             <1..1335
                     /locus_tag="AC16CM_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HIR3139521.1"
                     /translation="GLLAICLVLITTGAVAAAAHFVIGIPWAAAFVLGAVVAPTDPVA
                     AEATFRRLGVPDRVHTIVGGESIVNDGTALVAYNVAVAAVVTGTFSVVHAGLEFLLVS
                     GGGILLGAVVAVAVSPLYERLADPTIVVVFTLMPAYFSYIVAETLGASGILAVVTLGL
                     YVGWQVPKRFTARARVQSNSFWTVLTFLMDSLLFILVGLEFPIILEGISEYSFPQLLA
                     YAALVCGAVIGLRLLWFFFAPSSIPALDRLLRSTYLHAPWRERAVMGWCGMRGAVSLA
                     AALAIPLQTQAGAEFPYRDLILFLTYTVIFATLVFQGLTLPFLIRRLGVKDEGHEARM
                     AELQARVKATQAALTRLEEVSSDENIPARSQEQLRELYNERIRRYESGLEAGRVTDEY
                     RESSEVWTTWRRELFDAERNTIVTLRDRGEISADVMRRVERDIDLEELRQSG"
     gene            1349..1657
                     /locus_tag="AC16CM_06540"
     CDS             1349..1657
                     /locus_tag="AC16CM_06540"
                     /inference="COORDINATES: protein motif:HMM:NF023070.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139522.1"
                     /translation="MPTMFVLVNLKEDVTPAAYERWLEERYVPAVLGLASVDDWRGHR
                     VVRPGQGDSEPPYRYVATVEINDLERLGEDMDSEQVQALLGELGRYAEVTQLMTERFV
                     "
     gene            1685..>3137
                     /locus_tag="AC16CM_06545"
     CDS             1685..>3137
                     /locus_tag="AC16CM_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HIR3139523.1"
                     /translation="MPQIEIIVGLMVAVAALATLANRVKVPYPILLVIGGSVLGFVPG
                     LPKVRLDPELVFLLFLPPLLYVSAIFTSWRDFRANIRPITFLSVGLVLMTTFVVGAVA
                     HVATGLPWAAALVLGAIVSPTDAIAATAVAQRLGVPRRIVTILEGESLVNDATGIVAF
                     RVAVAAVVTGAFSIWGAGLQFVVGAAGGIAVGLAVGWLVIWTRRHISQDPSIQNIISL
                     LTPFVAYLTAEELPHTVWVQVHDLIGIPEDLHFSGVLAVVATGLYVGRKSPAVITSST
                     RLQGFGTWQLISFLLNGFIFILIGLQLDRVVSSLDEFTATDLVLYALLISVTVVVVRM
                     VWVFPATYVPRWVSRTLRERDPAPSWRSVSIIAWTGMRGVISLAAALSLPFQTASGAP
                     FPGRDLIIFLTFSVILATLVVQGLSLPPLIRALGLEDDHIGEREENKGRLKVADAALR
                     RLEELKEADCVREDTAERVEGLYNYRRNRFASRF"
BASE COUNT          484 a         1015 c         1031 g          607 t
ORIGIN      
        1 ggcttgctgg ccatctgcct ggtgctgatc accacgggcg ccgtcgcggc ggccgcgcac
       61 ttcgtgatcg ggattccctg ggctgctgcc ttcgtgctcg gtgcggtagt agcgcctacg
      121 gacccggtgg cagcggaggc gaccttccgc aggctgggcg ttcccgaccg cgtgcacacc
      181 atcgtgggcg gcgagagcat cgtgaacgat ggtacggcgc tcgtcgcgta caacgtggcc
      241 gtcgcagccg tggtgacagg gacgttctcg gtggtgcacg ccggcctcga gttcctgctc
      301 gtcagcgggg gtgggatact cctcggcgcg gtggtggccg tggccgtctc cccactatac
      361 gaacggctgg cggacccgac catcgtggtc gtcttcaccc tgatgccagc ctacttctca
      421 tacatcgtgg ccgaaacgct aggcgcctcc ggcatcctcg ccgtggtcac cctgggcctc
      481 tacgttggct ggcaggtccc gaagcgcttt accgccaggg ctcgcgtgca gagcaactcg
      541 ttctggacgg tacttacgtt cctcatggac tcgttgttgt tcattctggt ggggcttgag
      601 ttccccatca tactggaggg cataagcgag tactccttcc cgcagctgct cgcctacgcc
      661 gcgctcgtct gcggggccgt gataggtctc aggctcctgt ggttcttctt cgccccgagc
      721 tccatcccgg ccctcgacag gctgctccga tccacgtacc tgcacgcgcc ctggcgggag
      781 cgggcggtga tggggtggtg tggtatgcgg ggtgctgtct ccctggccgc cgcgctggcc
      841 ataccgttgc agacgcaggc cggggccgag tttccctaca gggatctcat cctcttcctt
      901 acctacacgg tcatcttcgc cacccttgtc ttccaggggc ttaccctccc cttcctcatc
      961 cgccgcctcg gagtcaagga cgaggggcac gaggcccgca tggccgagct ccaggcgcga
     1021 gtaaaggcca cccaggctgc gctgacgcgg ctcgaagagg tatcaagcga cgagaatatt
     1081 ccggcccgta gccaggaaca gttgcgcgag ctctacaacg agcgcatccg acgctacgag
     1141 tcgggactcg aagccgggcg cgtcacggac gagtacaggg agagctcgga ggtgtggacc
     1201 acctggaggc gcgagctctt cgacgccgag cgaaacacca tcgtcaccct gcgggatagg
     1261 ggtgagatca gcgccgacgt gatgcgccgc gtcgaacgcg acatagacct ggaagaactg
     1321 cgccagagcg gctgataggg ggaacgaaat gcccacgatg ttcgtgctgg tcaacctgaa
     1381 ggaggacgtg accccggcgg cgtacgagcg ctggctcgag gaacgctacg ttcctgcggt
     1441 gctcgggcta gcgtccgtag acgactggcg tggccacagg gtggtccgcc ccgggcaggg
     1501 agatagcgag ccaccctacc ggtacgtggc gacggtcgag atcaacgatc tggagcggct
     1561 cggcgaggat atggacagtg agcaggtgca ggcccttctc ggggagctgg gccgctacgc
     1621 ggaggtaacg cagcttatga cggagcgctt cgtctgagca gggaacgttg atggaggttg
     1681 tggtttgccg cagattgaga taatagtcgg tcttatggtg gcggtggcgg cgctcgccac
     1741 gctcgccaac cgggtcaagg tgccataccc gatcctgctc gttatcgggg gctcggtgct
     1801 cgggttcgtg ccggggctac cgaaggtgag gctcgacccc gagctcgtgt tcctgctctt
     1861 cttgccgccg ttgctctatg tctcggcgat attcacttcg tggcgtgact tccgggcgaa
     1921 catcaggccc atcacgttcc tctcggtcgg cctggtcctg atgaccactt tcgtggtcgg
     1981 cgccgtggca cacgtggcaa ccgggctacc ttgggcggcc gcgctggtgc tcggcgcaat
     2041 agtctccccc accgacgcca tcgccgccac cgcggtagcc cagcgcctcg gcgtcccccg
     2101 ccgcatcgtc acgatcctgg agggtgagag cctggtcaac gacgcgacgg gcatcgtcgc
     2161 cttccgggtg gccgtggcgg ccgtggtcac tggagccttc tctatctggg gggccggatt
     2221 gcagttcgtc gtgggagcgg ccggcgggat cgccgtcggc ctggctgtgg gctggctcgt
     2281 catctggacg cgccgccaca tctcccagga tccctccata cagaacataa tctcgctcct
     2341 cacgcccttc gtcgcctacc tgacggccga ggaattgccg cataccgtgt gggtccaagt
     2401 ccacgacttg atcggcatcc cggaggacct gcacttctcc ggcgtgctcg ccgtagtcgc
     2461 gaccggcctg tacgtcggcc gcaagagccc ggccgtaatc acttcgagta cacgcctgca
     2521 gggcttcggc acgtggcagc tcatctcttt cctgctaaac ggtttcatct tcatcctcat
     2581 aggtttgcag ctagatcgcg tggtcagcag cctggacgag ttcacggcca ccgacctcgt
     2641 cctctatgcc ctcctgatca gcgtcacggt agtggtggta cgcatggtct gggtctttcc
     2701 cgcgacgtac gtgccgcgct gggtaagccg taccctcagg gagagggacc ctgctccttc
     2761 atggcggagc gtctccatca tcgcctggac gggaatgcgg ggggtgatct cgctagcggc
     2821 ggccctgtcc ctgcccttcc agaccgcatc gggcgcgccg ttccccggac gcgacctcat
     2881 aatcttcctg acgttctcgg tgatcctggc gactctggtg gtccagggac tctccctgcc
     2941 cccccttata agggccctcg gcctggagga tgaccacatc ggagagcggg aggagaacaa
     3001 gggccgcctc aaggtggccg atgccgcact gcggcgcctg gaagagctga aggaggcaga
     3061 ctgtgtacga gaggacacgg cggagcgggt cgaaggcctg tacaactacc gccgcaacag
     3121 gttcgcatcc cgcttcg
//
LOCUS       DBNGVD010000234         4064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_48727, whole genome shotgun sequence.
ACCESSION   DBNGVD010000234 DBNGVD010000000
VERSION     DBNGVD010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_48727"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC16CM_06550"
     CDS             complement(<1..238)
                     /locus_tag="AC16CM_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139524.1"
                     /translation="MKTDLATVIRQANQRVVGHKKMLVLAIAMLALVSMAGCGGIMDL
                     AGDDPSPKKKQVANKNETKPKTVVVNEGMSKKEEK"
     gene            complement(448..1122)
                     /locus_tag="AC16CM_06555"
     CDS             complement(448..1122)
                     /locus_tag="AC16CM_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139525.1"
                     /translation="MAKTSLLRMTTLVVAGVVAMAFALVVSSVSAQAVQTGTRTFSND
                     QLIRIPANNLSGGTGGIANPYPSSIRVTGFKKVTDINVILRGVGHTDPDDLDVLLKGP
                     TGRTAILWSDAGGNTDILNVRVKLDDEASQFLPDSGQVFQGNFMTSNYEVGVDSWPGV
                     VQSDNRLLSRFDGTDPAGGWRLFVNDDFSQPDTGDRNGTGNFTGGWALQVKGVCTRAT
                     ADNRCR"
     gene            1642..2013
                     /locus_tag="AC16CM_06560"
     CDS             1642..2013
                     /locus_tag="AC16CM_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139526.1"
                     /translation="MALLRQTFGLILENCTGLPSAQAASTAGASRLLSQPDGDIALAV
                     VSVDTADGPDSGLIEHLHDLGLPVLAFGLKESEDLLARALEAGADDAISLHEAMAHFV
                     ARARQLAAPARSGPRLSARPG"
     gene            complement(2298..3254)
                     /locus_tag="AC16CM_06565"
     CDS             complement(2298..3254)
                     /locus_tag="AC16CM_06565"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3139527.1"
                     /translation="MARLAVLRGGHSMERDVSFLTGRRVQHALERLGHEVYPLDIEDP
                     TTATLMDLAPDAAFVCLHGPGGEDGTVQALLETLGIPYTGSGPLSSIRCMDKDYAKRA
                     LRANGIETPTSRTFLRKAMNEMGASAALEVAAEDLGYPFVVKPAREGSSFGLSRVEGP
                     EGLLDAVYEALGYDAKILLESWVDGTEISIPVIEPAGEDPRALGLVEIRPREGAYNFE
                     AKYTPGATDFAIPAEIPPEDAAHVEETALAAYRVLGCSGYARVDTIYAEGTTWVLDVK
                     TIPGFTETSTFPLAAEAAGISFETLVETILDAALHTEATAKL"
     gene            complement(3258..>4064)
                     /locus_tag="AC16CM_06570"
     CDS             complement(3258..>4064)
                     /locus_tag="AC16CM_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3139528.1"
                     /translation="LKRARTQGLHVKFLYTVPNFQNPAGVTMIAERRRELLEMAREFD
                     LVIVEDNPYGMLRFEGEPLPSLAALEQEEGDLDRVVYVGTFSKIFAPGVRLGWVHAQP
                     GILHKINVGKQGADLCSSNLSQMMIASYFQNADWRAYVKRLTTMYKERRDAMLDSLAE
                     FMPREVHWTHPEGGLFVWATLPSYLDATAMLPRAIAKNVAYVPGEGFYGGSPGTGKNN
                     MRLNFSFVEPERIRRGIELLSEVIRERMELRSDLERGSHRGVRSAVGTDG"
BASE COUNT          669 a         1303 c         1297 g          795 t
ORIGIN      
        1 tcttttcttc tttcttgctc atcccctcgt tgaccacgac ggtctttggt ttggtctcgt
       61 tcttgttggc gacctgcttt ttcttcggcg agggatcgtc cccggcgaga tccatgatgc
      121 cgccgcaacc ggccatgctc acgagggcta acatcgcgat tgccagtaca agcatcttct
      181 tgtgtccgac cactctctgg ttggcttgcc ggatcaccgt cgcgaggtct gtcttcacac
      241 ctataatcct cccttgatgg aacgcgtaaa ggccccatct tccctacgca gtgagggact
      301 attaacctgt gacgggagat gttgcagcgt acgacacggg cgtgttcggt gcctttggcc
      361 aggcgggtag gggggaagtc ttgcaaggtc aaggaggggg ccgggatccc gcgaaatcca
      421 gagaccccgg ccctccgcta cgctagccta tcggcagcgg ttatccgcgg tcgcgcgggt
      481 gcagacgccc ttgacctgga gcgcccagcc gccggtaaag ttgcccgtgc cgttcctgtc
      541 gcccgtgtcc ggctgcgaga agtcgtcgtt gacgaaaagc ctccagccgc ctgcggggtc
      601 cgtgccgtcg aacctggaga gcaacctgtt gtcgctctgg acgacgccgg gccaggagtc
      661 cacgccgacc tcgtagttcg aggtcatgaa attgccctgg aagacctgac cgctgtccgg
      721 aaggaactgc gacgcctcgt catccagctt gaccctgacg ttcagtatat ccgtgttgcc
      781 ccccgcgtcg gaccacagga tggcggtacg ccccgtcggt cccttcagca atacatcaag
      841 gtcatccggg tcggtatgcc cgacaccgcg caggatcacg tttatgtccg tgaccttctt
      901 gaagccggtt acccgtatgc tggaggggta ggggttggcg atcccgccgg taccacccga
      961 gaggttgttt gcgggtatcc tgatcagctg gtcgttgctg aaagttctcg ttcccgtctg
     1021 gaccgcctgt gccgagacag aggatacgac cagggcgaac gccatggcca ccacccccgc
     1081 caccaccaac gtcgtcatcc tcaacagact cgtcttcgcc aaggcatgtt ctcctctcct
     1141 ccgggttccg tacttatctg ctctgctatg acttacgcgc tgactggtct ctttcgccct
     1201 ccgatcgggg ttgttgccga gaacgccggc atgatcacaa gaaatctagt gcggcgtatc
     1261 acccaaatga gctaactttt gtaacgatcg gagattgtcg agcaccaact cggatgaggg
     1321 gaaggtctgc tgccgccgta tggcggagag cttttcgtat gccgcgaggc gcacgttggt
     1381 gcgtccaacg gccaggtttc accttccatc ggagcgtgga gcggtcgggt tttctgcggg
     1441 accttttccc ggttggggct tcgtctacct ggcgtcgatc cgatagagga aggcgattac
     1501 ccgctcccac ccggcctcgg acgaggcgcg gtggatttct tgtgatccgt acctcccgtt
     1561 gccccgtaaa gtacacgtgg accgccggag cagggggaga ccatcttcac gcgaacgaga
     1621 cgcatactga tcatcagcga cgtggcgctg ctacggcaga cgttcgggct gattctagag
     1681 aattgtaccg gcctcccttc agcgcaggcc gcctcgactg ccggcgcaag caggttactg
     1741 tcccaacctg acggtgacat cgccctggcg gtcgtcagcg tggataccgc agacggccct
     1801 gattccggcc tgatcgaaca cctccacgac ctcggcctgc cggtgctggc cttcggtctg
     1861 aaagaaagcg aggacctcct cgcgagggcc ctcgaagccg gtgccgacga cgctatctca
     1921 ctccacgaag ccatggccca cttcgtcgcc agggccaggc aactagccgc gccagcacgc
     1981 tccggccctc gcctgtcagc acgccccggc taacgccctc gctcgtcagc tcgcatcgcc
     2041 cggctccgcc tagagctcgc tgtcagcacg ctcccttcgg tcgctctcag ccggatccct
     2101 ctgcggcccg cactttacgt acaggatgtg tcggcacgta ggtaggctgc gaacctggct
     2161 caccgttacg gagagacgac tagccgaaag cgaggcgcgc cgaagcgttg ccgacaagcg
     2221 acagccgcca ggctggagcg tgctgacaag gcgcgaagcg ccgtgctgac gagtccgcgg
     2281 agcgggcgtg ccggaagtta cagcttcgct gtagcttccg tatgcagggc ggcgtcgagg
     2341 atggtctcca cgagggtttc gaaggagatg ccggctgcct cggcagccag ggggaaggtg
     2401 gaggtctcgg tgaagcccgg gatggtcttt acgtctagta cccaggtggt gccttcggcg
     2461 tagatggtgt caacgcgggc gtagccggag cagccgagta ccctgtaggc tgcaagcgcc
     2521 gtctcttcga cgtgggcggc gtcttccggc ggtatctccg cggggatagc gaagtctgtg
     2581 gcacccgggg tatacttggc ctcgaagttg tacgcgccct cgcggggccg aatctcgacc
     2641 aggccgaggg ccctcgggtc ctcgccggcc ggctcgataa cagggataga tatctctgtc
     2701 ccatccaccc agctctccag caggatcttg gcgtcgtacc caagggcctc gtagacggcg
     2761 tctaaaaggc cttcgggtcc ttctacgcgc gagagcccga agctggaacc ctcccgcgcg
     2821 ggcttgacca cgaacgggta ccccaggtct tcggccgcga cctcgagggc ggcggaggcc
     2881 cccatctcgt tcatggcctt tctgaggaac gtgcgggagg tgggcgtctc tatgccgttg
     2941 gcgcggagcg cgcgtttggc gtagtccttg tccatgcagc ggatgctgga gagcggcccg
     3001 ctccccgtgt aggggatgcc cagggtttcg aggagcgcct gcacggtgcc gtcttcgccg
     3061 ccggggccgt gcaggcagac gaaggcggcg tcgggggcga gatccatgag ggttgccgtg
     3121 gtcgggtctt cgatgtcgag gggatagacc tcgtggccga gacgttcgag ggcgtgttgc
     3181 acgcggcggc ccgtgaggaa ggagacgtcg cgctccatgg agtgtccccc gcgcagcact
     3241 gcaaggcgtg ccacgggcta accgtccgtg ccgacggccg agcggacgcc ccggtgcgag
     3301 ccgcgttcca ggtcgctccg tagctccatg cgctcccgga tcacctccga aagcagctct
     3361 atgccgcgcc ggatcctctc cggctccacg aacgagaagt ttagcctcat gttgttcttg
     3421 cccgtgccag gcgagccacc gtagaacccc tcgcctggca cgtaggccac gttcttcgct
     3481 atggcccgcg gaagcatcgc cgtcgcgtcg aggtaggatg gcagggtcgc ccacacgaag
     3541 agcccgccct cggggtgggt ccagtggacc tccctgggca tgaactcggc cagggagtcg
     3601 agcatcgcgt cgcggcgttc cttgtacatg gtggtcaggc gcttcacgta ggcgcgccag
     3661 tccgcgttct ggaagtagga ggcgatcatc atctgcgaga ggttcgagga gcagaggtcg
     3721 gcgccctgct tgccgacgtt gatcttgtgc aggatgccgg gctgcgcgtg tacccacccg
     3781 agcctgacgc cgggggcgaa gatcttcgag aacgtcccca cgtagacgac cctgtccaga
     3841 tccccctcct cctgctcgag cgccgccagg gacggcaggg gctcgccctc gaagcggagc
     3901 atcccgtagg ggttatcctc gacgatcacc aggtcgaact cgcgcgccat ctccagaagc
     3961 tccctacgcc tttcggcgat catggtcacg cctgcggggt tctggaagtt gggcacggtg
     4021 tagaggaact tgacgtgcag tccctgcgta cgggccctct tcaa
//
LOCUS       DBNGVD010000235         3711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_596014, whole genome shotgun sequence.
ACCESSION   DBNGVD010000235 DBNGVD010000000
VERSION     DBNGVD010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_596014"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16CM_06575"
     CDS             <1..909
                     /locus_tag="AC16CM_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3139529.1"
                     /translation="VTATANYLLVAFQLLVLGIFVALSIGYLVGTDTGATLTSPFFSP
                     DLTLPALAAGAAVAAYSYIGFDGLTLLAEETRDPRRTIPRAMIIVVLFFGALFVSISY
                     FAQLVHPGGTFENADSAALELARVVGGNLFASILLAGLIVGQFASGLAIQAGAARLLF
                     AMGRDGALPRGLSRVVPRYRTPAVAISLIGVTGLGALFLDVTTSTSFINFGAFSAFTL
                     VNLSVILHFLRHRPDSGVRGAFLWIVLPLAGAIVDFYLLLNLDGRAKLIGLIWLVLGV
                     LWLAWLTRGFREAPPQLSIDTNPEES"
     gene            941..1732
                     /locus_tag="AC16CM_06580"
     CDS             941..1732
                     /locus_tag="AC16CM_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3139530.1"
                     /translation="MTQTLKVVLAQLSPSLRRTDDNVQTMVRMMEERPRADLVLFPEL
                     FMSGYTTREVEGLAVDPDGPEVARVARAARDNSTAVVFGAPERFGSGYANSAIYVDRS
                     GSVAGIYRKAFLFGAEREAYVPGDELLVVDLGGPKAGLMICFDVEFPEVARALARAGA
                     DLLLTISANMDPFGRDHHVFCTSRALENGLPHAYVNQIGKGEEFTFAGGTMALSSDGD
                     ILAETSAPAEEVLEIDLELPARSTMRPDYLNQLRPPLAVSSQPEN"
     gene            complement(1736..2689)
                     /locus_tag="AC16CM_06585"
     CDS             complement(1736..2689)
                     /locus_tag="AC16CM_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3139531.1"
                     /translation="MSSRSWIPYAALLVVFWGVWGAFSGLPTAEYGYPDEMVYILWSL
                     TMLIPAFFILRSEGTFDRRPIAAVYGLLIGLTGAGGQLVLFKALTIGPAYLIFPIVAL
                     SPGITVLMATFLLRERLLGLTKVGVVLALIALVFFNISGASGDAGVGPWLILALLITL
                     AWGVQAYYMRKAATVGVNDGTTFGYMALSALLLVPVAFFMMGGFPEGAPWQAPTLTLG
                     TQLLNAVGALFLVMALSRGKASIVAPTTNALAPVLTVLISLAVYQTLPTVYGTLGILL
                     AIAGSTLMVYGDEKRGEVSGEEQAVIANETPGKSEVNRPTV"
     gene            complement(2686..3675)
                     /locus_tag="AC16CM_06590"
     CDS             complement(2686..3675)
                     /locus_tag="AC16CM_06590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR3139532.1"
                     /translation="MYLGVDGGGTKTALCLLRGDGRVAAEALAPSCYYFADGIGLVSR
                     VLRQGIEEVCQKASVTPADVRYAFFGLPTYGEVSGDVPALDAAPGAILGHDRYACDND
                     TICGWSGSLGGIDGINVVSGTGSITYGERAGNGVRVGGWGELFGDEGSAYWIAIKALN
                     AFSRMGDGRLPVGPLHETLRAHLALEADLDLVDVILNRWQGERREIAALSRVVSGAAE
                     AGDECASRILSGAAEELADLVDTTRRLLDFGQEEEIPVSYSGGVFNSRPVVEGFEVAL
                     GQRHDGYELRKPLFSPVLGAAIYAAKLAGEPLEVESIDPSEAARQDRKGEPER"
BASE COUNT          632 a         1277 c         1174 g          628 t
ORIGIN      
        1 gtgacggcca cggcgaacta cctgctggtg gccttccagt tgctggtact aggcatcttc
       61 gtcgcgctga gcatcgggta cctggtcggg acggacaccg gggccaccct gacgagcccg
      121 ttcttcagcc ccgacctgac gctcccggcc ctcgcggctg gcgcggccgt ggccgcctac
      181 tcctacatcg ggttcgacgg cctcaccctg ctcgccgagg agacccgcga cccgcgccgt
      241 accatcccgc gcgccatgat aatcgtggtc ctcttcttcg gcgccctgtt cgtaagcatc
      301 tcctacttcg cacagctagt ccaccccggg ggcaccttcg agaacgccga ctccgctgcc
      361 ctcgagcttg ccagggtcgt cggcggcaac ctgttcgcct ccatcctcct ggccgggctt
      421 atcgtggggc agttcgcctc tgggctcgcc atccaggccg gcgccgcgcg gctcctgttc
      481 gcgatgggac gcgacggcgc gctgccgcgc gggctaagcc gggtcgtgcc ccgctatcgc
      541 accccggccg tcgccatctc gctcatcggc gtcaccggcc tcggggcgct gttcctcgac
      601 gtcaccacct ccacctcgtt catcaacttc ggcgccttca gcgccttcac gctcgtgaac
      661 ctgagcgtga tcctgcactt cctgcgccac cgtccggata gcggtgtgcg cggggcgttc
      721 ctgtggatcg tgctgccgct ggccggcgcg atcgtcgact tctacctgct gctcaacctc
      781 gacggccggg ccaagctgat aggcctgatc tggctggtcc tgggggtgct ctggctggcc
      841 tggctcaccc gaggcttccg ggaggccccg ccccagctga gcatagacac caacccagaa
      901 gagagctgag aacggtctcc cgaagagagg agcattcctg atgacccaga cgctcaaggt
      961 ggtgctcgcc cagctgagcc caagcctgcg ccggacagac gacaacgtac agaccatggt
     1021 cagaatgatg gaggagcgtc cccgggccga tctcgtcctc ttccccgagc ttttcatgag
     1081 cggctacacg acgagggagg tagaagggct ggctgtagac ccggacgggc cggaggtggc
     1141 acgcgtggcg cgggccgcga gggacaactc gaccgcggtg gtgttcggtg cgccggagcg
     1201 cttcggaagt ggttacgcca actcggccat ctacgtagat aggagcggaa gcgtcgccgg
     1261 gatctaccgc aaggccttcc tgttcggggc agagcgggag gcgtacgttc ctggggacga
     1321 gctgctggtc gttgacctcg gcggtccgaa ggctgggctc atgatctgct tcgacgtgga
     1381 gttcccggag gtggcgaggg cgctggcgcg ggccggggcc gacctgctcc tgaccatctc
     1441 cgcgaacatg gacccctttg gacgcgacca ccacgtcttc tgcacctccc gcgcgctcga
     1501 aaacgggctc ccgcacgcct acgtgaacca gatcggcaag ggcgaagagt tcaccttcgc
     1561 gggaggaacc atggccctct cctccgacgg agacatcctg gccgagacca gcgcccccgc
     1621 agaagaggtg ctcgagatcg atctcgagct gccggcaaga agcaccatga ggcccgacta
     1681 cctcaaccag ctacgcccac cccttgcggt cagctctcag ccagaaaact gaccgctaca
     1741 ccgtcggcct gttcacctcg ctcttgcctg gggtctcgtt ggctatgacg gcctgctctt
     1801 ccccgcttac ctcgccgcgt ttttcgtccc cgtagaccat cagtgtagag ccggcgatgg
     1861 ccagcaagat accgagggtg ccgtagaccg tcggcagcgt ctggtagacg gcgagcgaga
     1921 tcaggacggt cagcaccggc gcgagggcgt tggttgttgg tgccactatg gatgccttgc
     1981 cgcggctgag ggccatgacc aggaacaggg cgccgaccgc gttcaggagc tgcgtgccga
     2041 gcgtgagcgt cggggcctgc cagggtgcgc cttcgggaaa gccgcccatc atgaagaagg
     2101 cgacagggac aagcagcagg gcgctcagcg ccatgtagcc gaaggtggtg ccatcgttta
     2161 ccccgaccgt ggccgccttg cgcatgtagt acgcctgaac gccccaggcg agcgtgatca
     2221 gcaacgccag gatcagccac ggccccactc cggcgtcgcc ggaggcgccc gagatgttga
     2281 agaagacgag cgcgatgagg gccagcacca cgccaacctt ggtcaggccg agaagacgct
     2341 ctctcagcaa gaaggtcgcc atgagcaccg tgatccccgg tgaaagggcc acgatcggga
     2401 agatgaggta tgccggtcct atggtgagcg ctttgaacag cacgagctgc ccgccggcgc
     2461 ccgtcagacc gatgagcagc ccgtagaccg ccgcgatcgg acggcggtcg aaggtcccct
     2521 cgctgcgtag gatgaagaag gctggtatca acatggtgag ggaccagagg atgtagacca
     2581 tctcgtccgg gtagccgtac tccgccgtgg gcaaccccga gaacgcgccc catacgcccc
     2641 agaaaacaac cagcaatgcc gcgtaaggta tccagcttcg tgagctcatc tttcgggttc
     2701 tcctttccta tcctggcgtg ccgcttctga tgggtcgatg gactcgacct ccagcggttc
     2761 gcccgcgagc tttgccgcgt agatggccgc ccccaggaca ggcgagaaca ggggcttcct
     2821 caactcgtat ccgtcatgcc gttgcccgag ggccacctcg aagccttcga cgacgggtcg
     2881 cgagttgaac acgccgccgg agtaggacac gggtatctcc tcctcctgac cgaagtcgag
     2941 caggcgcctg gtggtgtcta cgagatctgc tagctcttct gccgccccgg acaggatccg
     3001 ggacgcgcac tcgtctcccg cctctgcggc tcctgagacc acccggctta aggccgctat
     3061 ctccctccgt tcgccctgcc agcggttgag gatgacatcg acgagatcca ggtcggcctc
     3121 cagcgccagg tgggcgcgca gcgtctcgtg cagcggcccc acgggcagac gaccgtcgcc
     3181 catgcgggag aaggcgttaa gcgcctttat ggcgatccag taggcggagc cctcgtcgcc
     3241 gaagagctcg ccccatcccc cgaccctgac cccgttcccg gccctctcgc cgtaggttat
     3301 ggacccggtg ccgctcacga cgtttatgcc gtcgatcccg ccaagggaac ccgaccaccc
     3361 gcagatcgtg tcgttgtcgc aggcatagcg gtcatgcccg agaatggcac cgggtgccgc
     3421 atcgagggcc ggcacatcac cgctaacctc accgtaggtc ggcagaccaa agaacgcgta
     3481 gcgcacatcg gccggtgtga ccgaggcctt ctggcacact tcttcgatcc cctgccgcag
     3541 cacccggctc acgaggccta taccatccgc gaagtagtag caggaaggtg ccagcgcctc
     3601 cgcggcgacg cgcccgtccc cgcgaaggag gcaaagggcc gtcttggtgc ctcccccatc
     3661 gacgccgagg tacatcagga gctcccctcc tctatgggat gaatggagac g
//
LOCUS       DBNGVD010000236         5300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2418965, whole genome shotgun sequence.
ACCESSION   DBNGVD010000236 DBNGVD010000000
VERSION     DBNGVD010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2418965"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="AC16CM_06595"
     CDS             <1..582
                     /locus_tag="AC16CM_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR3139533.1"
                     /translation="LAFGEAEDVFVYDLKPDLGVVGPKYGRLVPGIRAALDAAPAEVG
                     IRAAGGESVTVDVEGDGITLSPEELLVEPRQREGYALEREGQLSVALSTELDQELLNE
                     GLVRELVHRVQNLRRENGFEIEEAIRVGLSGNPRVASLLDGRWGEYFKAEVLARELDL
                     APDDGGAPGDDFEDVTVDGETLRVRIQPLARAG"
     gene            641..1099
                     /locus_tag="AC16CM_06600"
     CDS             641..1099
                     /locus_tag="AC16CM_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139534.1"
                     /translation="MLGRKSRAEKLKEQAESNSLVPTAALSAAFAGARPVIERLLYDD
                     DLRDNIRTFIESARSIADELSDEDPQEIITRLWSDDKLRGQVEAAAGAASQGSRRVKG
                     ERVRTPRRRGGKLLFLLLLAALGFLFLNPRTGPQARKFVGDAYSSLTSGE"
     gene            complement(1154..1555)
                     /locus_tag="AC16CM_06605"
     CDS             complement(1154..1555)
                     /locus_tag="AC16CM_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139535.1"
                     /translation="MSEISLPLLGTLRLRSFPKIVGRAVGRVFGRTKAGPSQDPEMVS
                     LAASAIEEYAREHAALFEREERLRAKAERLAREGTPSESANNRAERARVEAEAGLLAL
                     RERFAPSAGERRRVFEREVVRLYPTLSFPHP"
     gene            1554..2135
                     /locus_tag="AC16CM_06610"
     CDS             1554..2135
                     /locus_tag="AC16CM_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139536.1"
                     /translation="MTHILNRPAPIVVILLSMILLSGCTGPFGSSPREQADEAISAAN
                     QSIEEHNELFDQARSTYSDVKNKIESGEDPSKEKENITAARTNLQEARTHLQDAEGSL
                     ATVDELDVDPAIKKYTSLLSEAMQDQLDAEAKEIDFYGILEQDPALADNREKALDLLS
                     EIGEGYQKAEETYREAQDLADKNPKLIEGSQGQ"
     gene            complement(2204..3064)
                     /gene="mutM"
                     /locus_tag="AC16CM_06615"
     CDS             complement(2204..3064)
                     /gene="mutM"
                     /locus_tag="AC16CM_06615"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR3139537.1"
                     /translation="MPELPEVTVISEDVAALALGRRVLRAAVFRPDVTNVDPEEFGSR
                     LVGKTLEATGRRGKMIVLDFGEVVGLVHLVISGRVLRLPAWQEPDRMNTAVLEFADEG
                     EPVVLAFTRLWLGYFDLYEPGDENNHPLLARLGPDPFSDGFTVEYLVSALDRKANTKG
                     LLLDQSVVSGLGNIYVDEVLFAARVNPTRRANTLTQEEIEKLHTATRDILGRAIQARG
                     TTFDSYHDAFGETGQYQHQLQVFTHAGEPCPRCGTEISKSRVAGRGTYTCPTCQPSEP
                     SAVSRQPLAD"
     gene            complement(3065..3907)
                     /locus_tag="AC16CM_06620"
     CDS             complement(3065..3907)
                     /locus_tag="AC16CM_06620"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3139538.1"
                     /translation="MAQEQSGNGLGFELGAFDLDGTVLRRNLRITKETIAALDRLRER
                     GMRLVVATGRRFEDAREHAERLGFEEGDPVICYGGSMVRRINGETLLHRTLPTELGME
                     VLDWAARRDLHARVFVDGGIVTSPDTPATMEHLRGYEDPGVDAVESPVRWLEEGGVEP
                     TKLVIVDYPDEIGGWLEEARTTFAGRLFVTRSLPHYVEIGGLEGTKSMALRFLCERWG
                     IEQDRVLAFGDADNDIDMLRFAGRGVAVGGMTVEVREAADEVVPGVDEDGVACYVDKL
                     LEKS"
     gene            complement(3983..5065)
                     /locus_tag="AC16CM_06625"
     CDS             complement(3983..5065)
                     /locus_tag="AC16CM_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HIR3139539.1"
                     /translation="MSDTRSRTAGVAVWLALGALTAPAGYLAQRAGLPAGWLVGPMLA
                     ALVVALLWEKHPTVPRWGRVASLAVVGGVLASTFRPSVVPLISRHLLPVILVVCGTLV
                     LSLGVGLLLPALIHLDRKTAALGTLPGAASGMLAMSDPLGADARLVALMQYTRVVVVV
                     VTATLVGRLGLEAGEGSAQSTVQGLQPAPGGTDLLVQNDLTTYALTALVAGIGAWAGT
                     RVRLPAGALLGPLILGISLEELGVMHLAWPQGVPQVAYLVIGLWVGLLFDGDSIKRAG
                     KLLPVMVLSSTCLVVACGVLGWILSAITGIDPITAFLATTPGGIDSVAIVALGSGADA
                     PLVLAIQMLRVLAVIAVGSFLGWLWS"
     gene            5157..>5300
                     /locus_tag="AC16CM_06630"
     CDS             5157..>5300
                     /locus_tag="AC16CM_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139540.1"
                     /translation="MELGLEGKVALVTGGSKGVGRGVADALAAEGCHLCICSRNEQEV
                     RKAA"
BASE COUNT         1002 a         1727 c         1738 g          833 t
ORIGIN      
        1 ctcgccttcg gggaggccga ggacgtgttc gtctacgacc tgaagcccga ccttggcgtc
       61 gtcggcccga agtacggaag gctcgtgccc ggcataaggg cggcgctcga cgcggccccc
      121 gccgaggtcg gcatccgcgc cgccggcggc gagagcgtta ccgtcgacgt ggaaggagac
      181 gggatcaccc tctcaccaga agagctgctc gtcgaaccca gacagaggga gggttacgcc
      241 ctggagcggg agggccagct ctcggtggcc ctgagtaccg agctcgacca ggaactgctc
      301 aacgaaggtc tggtcaggga gctggtgcac agggtgcaga acctccggcg cgagaacggc
      361 ttcgagatag aagaggccat cagagtaggc ctctcgggca acccgagggt cgcttcccta
      421 ctagatggcc ggtggggcga atacttcaaa gccgaggttc tggccagaga actcgacctg
      481 gctcccgatg acggcggcgc tcccggcgac gacttcgagg acgtgacggt tgacggggag
      541 accctgcggg taagaatcca gcccctcgcc agggcagggt agatccgtgg cgaaacgggt
      601 atgatgtacg gacgaagaga tcaggacagg gaggtttagc ttgttgggaa gaaagagccg
      661 tgcggagaag ctaaaagagc aggccgagtc caattccctg gtcccgacgg cggcgctcag
      721 cgcggccttc gccggggcga ggcctgttat agagaggcta ctctacgacg acgacctcag
      781 ggataacatc cgtaccttta tcgaatcggc ccgctccatt gccgacgagc tctccgacga
      841 ggacccgcag gagatcatca ccaggctctg gagcgatgac aagctccgtg gccaggtcga
      901 ggccgcggcc ggtgccgcca gccagggcag caggcgggtg aagggcgaga gggtccgtac
      961 gccccgccgt aggggcggca agctgctgtt cctcctgctt ctcgcggccc ttggcttcct
     1021 gttcctgaac ccgagaacgg ggccccaggc acgcaagttc gtgggggacg cctacagttc
     1081 cctcacctcg ggggaataga agcgaaaaag tcaccgcccg gccgccccgt gggcagtgcc
     1141 gccggtggtg tcatcacggg tggggaaacg agagagttgg gtagaggcgc acgacttcgc
     1201 gctcgaagac gcgtctcctc tcccccgccg aaggcgcgaa ccgctcgcga agagcgagaa
     1261 gtccggcttc ggcctcaacc ctggcgcgct ccgcacggtt gttggcgctc tcgctcgggg
     1321 ttccctcccg cgcgagccgt tcggccttcg cccgcaaccg ttcttcccgc tcgaagaggg
     1381 cggcgtgttc cctggcgtac tcttctatgg ccgaagccgc cagcgaaacc atctcagggt
     1441 cctggctggg ccctgctttc gtgcggccga agacccggcc aaccgcacgt cccactatct
     1501 tcggaaagga gcgtagcctg agggtgccca acaaaggtag actgatctca ctcatgaccc
     1561 acattctaaa ccgcccggcg ccgattgtag taatcctgct ctcaatgatt ctcctctccg
     1621 ggtgcactgg ccctttcggg agcagcccgc gcgaacaggc cgacgaggct attagcgccg
     1681 ccaaccagtc catcgaagag cacaacgagc tattcgacca ggctcgctcc acctactcgg
     1741 acgtcaagaa caagatagag tcgggcgaag atccctccaa agagaaggaa aacataacgg
     1801 ccgccaggac caacctgcaa gaggcgcgca cacacctgca ggatgccgaa ggatccctgg
     1861 cgaccgtgga cgaactggac gtcgaccctg ccatcaagaa gtacaccagc ctcctctccg
     1921 aggccatgca agaccagctg gacgccgagg ccaaagagat agatttctac ggcatcctgg
     1981 aacaggaccc ggccctggcg gacaatcgcg agaaagccct ggacctgctc tccgagatcg
     2041 gagaaggtta ccagaaggcc gaagagacct accgcgaagc ccaggacctg gccgacaaga
     2101 acccgaagct gatagaggga tcacagggtc aataaagctg tcagccgtcg gctttcagct
     2161 attctggctt gcccgcgcag gcgtcgcgcg cccgacctac cagctaatcc gctaatggct
     2221 gacggctaac agctgacggc tcagagggct ggcaggtggg acaggtgtag gttcctcttc
     2281 cggcgacgcg ggatttcgag atttcggtgc cgcagcgggg gcatggctcg ccagcgtggg
     2341 tgaagacctg gagctggtgc tggtactggc ccgtctctcc gaaggcatcg tggtaggagt
     2401 cgaaggtggt gccgcgggcc tgtatggcgc ggcccaggat gtcgcgggtg gcggtgtgga
     2461 gcttctctat ctcctcctgg gttagggtgt tggccctgcg ggtggggttg acgcgggcgg
     2521 cgaagaggac ctcgtccacg tagatgttgc cgagccccga gaccaccgac tggtcgagta
     2581 gcaggccttt tgtgttcgcc ttgcggtcga gggcggagac caggtactcg accgtgaacc
     2641 cgtcggagaa ggggtcgggg cccaggcgcg cgagtagtgg gtggttgttc tcgtcgccag
     2701 gctcgtagag gtcgaagtaa ccgagccaga gccgggtgaa ggccagcacc acgggttcgc
     2761 cttcgtccgc gaactcgaga accgccgtgt tcattctgtc cggttcttgc caggcgggca
     2821 ggcgcaggac ccttccggag atgaccaggt gcacgaggcc gaccacctcg ccgaaatcga
     2881 ggactatcat cttgccccgt cgtccggtgg cctctagcgt ctttcccact agccggcttc
     2941 caaactcctc ggggtccacg ttcgtcacgt ccggcctgaa gacggcggcc cttaggaccc
     3001 tgcgacccag ggcgagggcg gccacgtctt cggagatgac cgtcacctcc ggcaactctg
     3061 gcatctagct tttctccagc agcttgtcca cgtagcaggc gaccccgtcc tcatccacgc
     3121 ccggcaccac ctcgtctgcg gcctcgcgga cctcgacggt catgccgcca acggcgaccc
     3181 cgcgcccggc gaagcggagc atatctatgt cgttgtccgc gtcgccgaag gcgaggaccc
     3241 tgtcctgttc tatgccccac cgttcgcaca ggaaccggag ggccatggac ttggtgcctt
     3301 cgaggccgcc gatctcgacg tagtggggca ggctacgggt gacgaagagc ctcccggcga
     3361 aggtggtccg cgcctcctcc aaccagcccc cgatctcgtc ggggtagtcc acgatcacga
     3421 gcttggtcgg ctccacgccg ccctcctcga gccaacgcac cggggactcc acggcgtcga
     3481 cgccgggatc ctcgtagccc ctgaggtgtt ccatggtcgc gggggtgtcc ggcgaggtga
     3541 cgatcccgcc gtcaacgaac acgcgcgcat ggagatccct cctggccgcc caatccagca
     3601 cctccatgcc aagctccgtg ggtagcgtgc ggtgcagcaa ggtctcgccg ttgatcctac
     3661 gcaccataga gccgccgtag cagatcaccg ggtccccctc ctcgaaacca agccgctccg
     3721 cgtgctccct cgcgtcctcg aaacgcctgc ccgtggccac gacgagcctc attcccctct
     3781 cgcggagacg atccagggcg gcgatggttt ccttcgtgat cctcaggttc cgccgcagca
     3841 cggtcccgtc gaggtcgaag gcgccgagtt cgaagcccag gccgtttccg ctttgttctt
     3901 gagccaaata caacctttca gccgtcgtgg ggacgagaag ataaccggat aacgcccccc
     3961 gtttcgacca gtttttagca catcacgacc acagccaccc gaggaacgag ccgaccgcga
     4021 tcacggccag gacacgcagc atctgtatgg ccaggacaag cggcgcgtcg gcgccgcttc
     4081 cgagcgccac gatggcaacg gagtcgatcc caccgggcgt ggtcgccaga aaggccgtga
     4141 tcggatctat gcccgtgatc gcgctcagga tccacccgag gacgccgcac gcgacgacga
     4201 ggcaggtaga ggagagaacc attaccggca acagcttccc cgcccgcttt atagagtcgc
     4261 cgtcgaagag gagcccgacc cacaagccga tgacgaggta ggccacctgc ggcacgccct
     4321 ggggccaggc caggtgcatc acacccagct cttcgagaga gatcccgagt atcaggggac
     4381 ctagcagagc cccggcgggc agccggaccc tcgtgccggc ccacgcgccg atcccggcga
     4441 ccagggcagt aagggcgtag gtagtgaggt cgttctggac cagaaggtcg gtgcctcccg
     4501 gggccggctg caggccctgg accgtggact gtgccgaccc ttctcccgcc tccaacccta
     4561 ggcgcccgac cagcgtggcc gtcacgacca caacgacgac gcgggtgtat tgcatcaggg
     4621 ccacgaggcg cgcgtcggcg ccaagcgggt cgctcatggc gagcatcccc gaggcggccc
     4681 cggggagcgt cccgagggcc gccgtcttcc tgtcgaggtg aatcaaggcc gggagcaaca
     4741 gccctacccc gaggctcaga accagggtgc cgcataccac gaggatcacc ggcagcaggt
     4801 ggcgggagat caggggcacc acagatggcc tgaaggtgga ggcgagcaca ccccccacca
     4861 cggcgagcga tgctacgcgt ccccagcgcg gcaccgttgg atgcttctcc cagagaagcg
     4921 ctacgacgag cgcagcgagc atgggtccga ccagccaacc ggcgggcagg ccggcccgct
     4981 gggccaggta tcctgccggg gccgtcaggg cgccgagggc cagccatacg gcgacaccgg
     5041 ccgttcgtga tctcgtgtca gacatctcgt cataaggtac tacggcgttt cgggaaggtg
     5101 tgctggggca acaatggcca gacgtagagg tcgaccgttt ctggaaggag cccgcgatgg
     5161 agctgggact ggaaggcaag gtggcgctgg taaccggcgg gagcaaggga gtaggacggg
     5221 gtgtcgccga cgcccttgct gcagaggggt gtcacctctg catctgctcc cgcaacgagc
     5281 aagaggtgcg aaaggctgcc
//
LOCUS       DBNGVD010000237         2140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_779452, whole genome shotgun sequence.
ACCESSION   DBNGVD010000237 DBNGVD010000000
VERSION     DBNGVD010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_779452"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="AC16CM_06635"
     CDS             complement(<1..835)
                     /locus_tag="AC16CM_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3139541.1"
                     /translation="MTAFPLWAWFGFTALIAVLLALDLLVLARGAREISFRRAALLSV
                     FWVGVAMLFGVLVFVVAGPARGTEYLTGYIVEKSLSVDNVFVFALIFSYFAVPARYQY
                     RVLLWGVVGALVLRGVFVLLGAGLLERYDWMVYVFGVFLIYTGIRMALHRDTEVHPDR
                     NPILRLARRLVPISEGYEGEKFFVRKAGVLMATPLFAVIIVVGTTDVIFAVDSIPAIF
                     AITSSAFVVWSANAFAVLGLRPLYFMLAGMIDRFVYLSLGLSVVLVFVGAKFIYSGLF
                     GK"
     gene            1200..1853
                     /locus_tag="AC16CM_06640"
     CDS             1200..1853
                     /locus_tag="AC16CM_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR3139542.1"
                     /translation="MVLLCTALGAGLSLAASIRARGLRRSVLLLVLGTGLTATGELLA
                     TGPLKLLRHRLRYRLVGVPFAILLGWYAVIHGSLVIARRVAERTRSGEDATKTVPVLA
                     ALVGVSLDLILDPAGLDLGLWKWNSDGTYAAEVAGPNGHVGVPLVNYLGWASLVGGAT
                     YLYGSACEDKAEDPLPALLLLPLYLAAVGWAIERRKYRYILLSAPFPVALYVALQKG"
     gene            1888..>2140
                     /locus_tag="AC16CM_06645"
     CDS             1888..>2140
                     /locus_tag="AC16CM_06645"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR3139543.1"
                     /translation="MSPKPVILDVDPGHDDAVAMMLACGHPDLDLLAVTTVAGNVSLD
                     KTTRNALRVLSLIGCDDVPVGRGASGPLERPLYTAEDIHG"
BASE COUNT          403 a          722 c          687 g          328 t
ORIGIN      
        1 ccttgccgaa gagaccagag tagatgaact tcgccccgac gaagacgagc accacggaga
       61 ggccaaggct caggtagacg aagcggtcga tcatcccagc aagcatgaaa tagaggggtc
      121 tcaggccaag gaccgcaaag gcgttcgccg accagacgac gaacgcgctg ctcgtgatgg
      181 cgaagatcgc cggtatggag tcgacggcga agatcacgtc ggtggtcccg accacgatga
      241 tgaccgcgaa cagcggggtc gccattagaa ctcccgcctt tcgaacgaag aacttctcgc
      301 cctcgtagcc ctccgagatc ggcacgaggc gccgcgcgag gcgcaggatg gggttcctgt
      361 cgggatggac ctcggtatcg cggtggaggg ccatccttat gccggtgtag atcaggaaca
      421 cgccgaagac gtagaccatc cagtcgtagc gctcgagcag accagccccg aggaggacga
      481 agacgccgcg cagcacgagc gccccgacca cgccccacag aagcaccctg tactggtagc
      541 gggcgggcac ggcaaagtag gagaagatga gggcgaacac gaacacgttg tcgaccgaga
      601 ggctcttctc gacgatgtag cccgtcaggt actcggtgcc gcgtgcgggg ccagccacga
      661 cgaagacgag gacaccgaag agcatcgcaa cgcctaccca gaaaacgctc agcagcgcgg
      721 ccctgcggaa ggagatctca cgcgctccgc gggcgagcac cagcaagtcg agcgcgagga
      781 ggaccgcgat cagggccgta aacccaaacc aggcccacaa cggaaacgcg gtcatgaggg
      841 gccccccggg tcggacgtga gagagcggtc ctggggcggg agcggaatcg acatagccca
      901 tctcctctcg aacgggccgc gaaacgcgaa aaagcgagcc atcgcagcct tttgcctgcc
      961 gatggtctcg cccattgcgc ctcagcgccg gtacccggcc gggggcttcg ccccgtattg
     1021 acgaccggtc cccccgctaa ggcgggtggc tactccccat tggcaatgac tttacccaac
     1081 aaaaggccgg tcgtcaacaa gagaagataa agctcctcca gcggcgtatg ctgtagacgg
     1141 tcggctgggt ttggcttgga ggtccctctc tgaacgacgc agatcccagg ttatcgaggt
     1201 tggtcctcct ctgcaccgcc cttggcgccg gcctgagcct ggcggcctcg atcagggccc
     1261 gcggcctccg ccgctccgtg cttctcctgg tgctcggtac cgggctgacg gccacgggcg
     1321 agctcctcgc cacgggcccc ctgaagctgc tgcgccaccg tctgcgttac cggctggtgg
     1381 gcgtgccctt tgccatcctg ctcggctggt acgctgtcat ccacggctcc ctcgtcatcg
     1441 ccaggcgggt tgcggagcgc acccgttccg gagaggatgc cacgaagacc gtcccggtcc
     1501 tggctgcact cgtcggcgtc agcctcgacc tcatccttga cccagccggc ctcgacctcg
     1561 gcctctggaa gtggaacagc gacggtacct acgcagcaga ggtggccggc ccgaacgggc
     1621 atgtaggggt gccgctcgtc aactacctgg gttgggcctc gctggtcggg ggcgccacct
     1681 acctgtacgg gtctgcttgc gaggacaagg cggaggaccc tctccccgcg ttgctcttgc
     1741 tacccctcta cctggcggcg gtcgggtggg ccatcgagcg gcgcaagtac cgttacatcc
     1801 tcctctcggc gccgttcccc gtcgcgctgt acgtggcgct ccaaaagggc taagttacca
     1861 tcctgtaaga gatcgagagg agccaaggtg agcccgaagc ccgtgattct tgacgtagac
     1921 cccggccacg acgacgctgt ggccatgatg ctggcctgcg gccaccctga cctcgacctg
     1981 ctcgccgtca cgaccgtcgc cggcaacgtc tccctcgaca agaccacgcg caacgccctg
     2041 cgcgtcctct ccctcatcgg gtgcgacgac gtgcccgtcg ggaggggggc ttccgggccc
     2101 ctggagaggc cgctctacac cgccgaggac atccacggcg
//
LOCUS       DBNGVD010000238         1865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2419702, whole genome shotgun sequence.
ACCESSION   DBNGVD010000238 DBNGVD010000000
VERSION     DBNGVD010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2419702"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1011)
                     /gene="trpS"
                     /locus_tag="AC16CM_06650"
     CDS             complement(19..1011)
                     /gene="trpS"
                     /locus_tag="AC16CM_06650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3139544.1"
                     /translation="METKQRVFSGIQPSGNFHLGNYVGALKNWVAIQDEYENFFCIVD
                     LHAITVPQDPAVLRHKIREVAAIYLASGLDPEKCVIFRQSHVSAHAELCWLLNSVARF
                     GELSRMTQFKDKTTKGGVESASAGLFDYPVLMAADVLLYNAHLVPVGDDQRQHLELMR
                     TLARRFNHLYGETFVVPEAMIPPTGARVMALDEPGEKMSKSSPRPASYVGILDDPGAI
                     RKKIRRAKTDSGTEITASEDKPAITNLLDIYSAMTGEPVPDIEAEYEGKGYGDLKKDL
                     AEVVVEGLAPVRDRANELLADPSELDRLLESGARRAESAAQPTLRHVQANLGLD"
     gene            1156..>1865
                     /locus_tag="AC16CM_06655"
     CDS             1156..>1865
                     /locus_tag="AC16CM_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7800 domain-containing protein"
                     /protein_id="HIR3139545.1"
                     /translation="MPELVLGPLLRYAGETDATVWVETDAACEVEVLGCSSPTFRVGS
                     HHYALVHVTGLEDACEYGVALDGEEVWPEADSRFPPSVIRPLKSGEALKLALGSCRIS
                     APHEPPYTLNHREDERGLGIDALYATAMDLKDRPPEDLPHLLVMLGDQIYAHKPPFDT
                     LRFIEGRRDTAEPPGEVVADFEEYARIYRDAWSDPAIRWLLSTVPSAMIFDDHEVGDD
                     WNISQAWVEEMRQHPTWN"
BASE COUNT          311 a          625 c          591 g          338 t
ORIGIN      
        1 cggagtcgtt gcaacgggct aatcgaggcc caggttcgcc tgaacgtgcc gaagggtcgg
       61 ctgcgcggcg ctctccgcgc gcctcgcccc ggactcgagc aggcggtcca gctcggaggg
      121 gtcggccaga agctcgttgg cgcggtccct gacgggcgcg aggccctcca ccacgacctc
      181 ggccaggtcc ttcttcaggt cgccgtagcc cttgccctcg tactcggcct cgatgtctgg
      241 gacgggctcg ccggtcatgg cggagtagat gtcgagcagg ttggttatgg ccggtttgtc
      301 ctcggaggcc gtgatctccg tccccgaatc cgtcttggcg cgccggatct tcttccttat
      361 ggcgccaggg tcgtccagga tgccgacgta gctggccggc ctaggagagc tcttgctcat
      421 cttctccccc ggctcgtcga gcgccatcac cctcgccccc gtaggcggga tcatggcctc
      481 cggaaccacg aaggtctcac cgtagaggtg gttgaacctc ctggcgagcg tccgcatgag
      541 ctccaggtgc tgtctctgat cgtcaccaac gggcacgagg tgcgcgttgt agagcagcac
      601 atcggcggcc atgagcaccg gatagtcgaa gaggcccgcg ctcgccgact cgacgccgcc
      661 cttggtcgtc ttgtccttga actgcgtcat gcgcgagagc tcgccgaaac gggccacgct
      721 gttcagcagc cagcacagct ccgcgtgcgc gctgacgtgg ctctggcgga agatcacgca
      781 cttctcgggg tcgaggccag aggcgaggta gatcgctgcc acctcccgga tcttgtgcct
      841 taacaccgcc ggatcctgcg gcaccgtgat ggcgtgcaaa tccacgatgc agaaaaagtt
      901 ctcgtactca tcctgtatgg caacccagtt cttgagcgcc cccacgtagt tgcccaaatg
      961 gaagtttccg ctcggttgaa tcccgctgaa taccctctgt ttcgtctcca taaccacatt
     1021 ctaacggacg caccagggtc cgcctatcgc gcccggtgcc ggacgcgctg acggctgacc
     1081 gctctaacag gcctgatgta tcctgtgctc ctgcctgaga ggggaaaacg ctgatagacc
     1141 agatagagaa gcacgatgcc tgagctcgtc ctcgggccgc tcttgcgcta cgcgggggag
     1201 acggacgcga ccgtgtgggt agagacggac gccgcctgcg aggtggaggt actcggttgc
     1261 tcctcgccca cgttccgcgt cggaagccat cactacgccc ttgtccacgt tacgggcctg
     1321 gaggatgctt gcgagtacgg ggtcgccctt gacggtgagg aggtgtggcc cgaggcggac
     1381 tcccgcttcc cgccgagcgt gatccggcct ctgaaaagcg gggaggccct gaagctggcc
     1441 ctcgggtcct gtcgcatctc agccccgcac gagccacctt acaccctgaa ccatcgcgag
     1501 gacgagcggg gcctcgggat cgacgccctg tacgccacgg cgatggacct gaaggacagg
     1561 ccccccgaag acctaccgca cctcctcgtg atgctggggg accagatcta cgcccacaaa
     1621 ccccctttcg acaccttgcg attcatcgag ggccggcgcg acaccgcgga gccccctggc
     1681 gaggtcgtgg ctgacttcga ggagtacgcc aggatctacc gcgatgcctg gtcggatccg
     1741 gcgatacgct ggctgctctc caccgtgccg tcggccatga tcttcgacga ccacgaggtc
     1801 ggcgacgact ggaacatctc gcaggcctgg gtggaggaga tgcgccagca ccccacctgg
     1861 aacga
//
LOCUS       DBNGVD010000239         5592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_233108, whole genome shotgun sequence.
ACCESSION   DBNGVD010000239 DBNGVD010000000
VERSION     DBNGVD010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_233108"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1096
                     /locus_tag="AC16CM_06660"
     CDS             146..1096
                     /locus_tag="AC16CM_06660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR3139546.1"
                     /translation="MLKGHVLRPGGTIGVPAPASPYHNRSEVLRGVEWWEDQGYRVKL
                     GRNVFARDAYVAGTPEGRAEDLMDMFTDDEVDVVHCFQGGYGAAQTIELLDFDAIGQN
                     PKPFIGYSDITALHTALRHYTGLVTFYGPGLAGVNDRETKKFTQERLLKALTSEEPLG
                     EVPRDPKNTYIRSFGGGSVTAPMVGGCLWLLCQAIGTPWQPDFDGRILFFEDVDAPPW
                     YIDGFLNQMRQAGVLDGVAGVVVGELEKCDWREERPEFPQTLSIEDVLERYIEPLGVP
                     ALYGYPLGHGERLCTIPLGVSAVLNGDDRTLTIQEPALLE"
     gene            1153..2943
                     /locus_tag="AC16CM_06665"
     CDS             1153..2943
                     /locus_tag="AC16CM_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3139547.1"
                     /translation="MVVVLVALVSVLAGIASAAAQDSSGEDEKVVFRVGATNDIDGFN
                     PFKIVEIPSYEVMGLTYDLLVETSPKDSSPAPGLAESWETSDDGLTWTFHLNKDARWH
                     DGKPVTSEDVAYTYQRILDEEQGLYIDAVKQIDSIETPDKHTVVFTTKKPSVQMLNTY
                     VYILPKHIWKDVPADETKSFKNEPVIGSGPFQAVEWREGQFARLEANPDYFRGAPHVD
                     EVIFQFYDNADTMVQALKNGEVDYIYNMPINLFKSLQNQEGIETNSAPDPGFTELGFN
                     LYEPTPEAIEEFGAPEKTTGNPALLDPRVREAINWAIDEEVLTDKVLQGEGVAGSTLV
                     PPALATYHLELPESDIQGFDIERSRELLAQAGWEDTNGDGTVDKGGEELQLRLFLRTE
                     DSDSATQGAFLEDWFEKAGIGLETKVMGDTALTDAIYAADYDMFIWGWGSEADPDFIL
                     SVLTCDQIMGWSDTFWCNDRYSRMYAEQKEQLDLDERAATVKEMQRIAYEDNPYVILY
                     YDNQLEAWRTDKFKGWSKTPSDADPGQVAFTFTTKTYMDLQPLSAGGAGGDESGGTSP
                     LLFVGLGVVAIAVLAGIVVLIRRGSAEDRA"
     gene            2958..3917
                     /locus_tag="AC16CM_06670"
     CDS             2958..3917
                     /locus_tag="AC16CM_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017975881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139548.1"
                     /translation="MGGKLLQAAGTLLFVLVLNFFLFRILPGDPERTFIRLQRISPSQ
                     IDEVEREFGLDRPLWVQFFDYLGDTARGELGISYVFSRPVAEVIGTALWPTILLVGVA
                     TLAATVIGTLMGIWGAWRRGSPIDTSLLGFGLVTYAMPEFWLGMLLILGFAVALGWFP
                     TSFMTTPGLVDASAGAQLVDTMEHLFLPALTLTLALIGEYALIMRSSVMEAMNEEYVT
                     TARAKGMREALILRKHIVPNALLPLVTLAALNIGFIVSGAIAVETVFSWPGLGLLTFR
                     AINGGPDYPLLQGLFLLFSVAVILANLLAALLLGLLDPRIGRA"
     gene            3944..4819
                     /locus_tag="AC16CM_06675"
     CDS             3944..4819
                     /locus_tag="AC16CM_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139549.1"
                     /translation="MVGRARRIRAFKSSARLFARNKMGVAGLTILTVFVLLAVFADVL
                     VPQASLSVTNAPGTPLEPPSARFWLGTDERGRSVLDLVIQGSQISLLVGFLASAVSML
                     IGSLVGIVAGYVGGWIEVVLMRFTDWFLVIPFLPLAIVLVAILGRGLFVIAFVIGITT
                     WPGTARVVRAQVLTLKERSYVERARALGASNSQIVTRHILPNVFPLIFANTILIVAIA
                     IYTEAILSFLGLGDPFSISWGSILESAFAEGAISLGAWWYLVPPGLCIALVVLAFAMC
                     GYAFDEILDPRLRDR"
     gene            4816..>5592
                     /locus_tag="AC16CM_06680"
     CDS             4816..>5592
                     /locus_tag="AC16CM_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139550.1"
                     /translation="MTETAAPTGTLLEVKNLRVLYEVRGTKAPAVDGVNLSLQPGDAL
                     GLAGESGCGKSTLALACLGLLPRNATLEGSIRYRETEVIGAKPGRLRALRWAGISMVF
                     LGAMSAFNPVKRVGDQVVEAILLHEDVSRGEARRRTEELFEQVGIPPRRAGDYPHEFS
                     GGMRQRAMIAMALACNPPLVIADEPTTALDVMIQAQILNLLARLRDERNIALLVITHD
                     LSVLAQIADRVAVMYAGRIVEEGSAAELLTSPRHPYSKSLV"
BASE COUNT          999 a         1801 c         1784 g         1008 t
ORIGIN      
        1 tctcgacgtc gggcgtccag ggtgacgccc gaggggcgac cgccgcaaaa ggtggggcgc
       61 tgttcgcggc cgccacggcg gaaatagcct cctacgtcac ggagttgagg agcatgccga
      121 tcccacatag aaaggatcac cactgatgct caagggccac gtcttgcgtc ccggaggaac
      181 gatcggggta ccggctcccg cgagccccta ccacaaccgc agcgaggtac tgcgcggggt
      241 tgagtggtgg gaggatcagg gctacagggt gaagctgggc cgcaacgtgt tcgcccgcga
      301 cgcctacgtg gccggcacgc cggagggccg ggccgaagac ctaatggaca tgttcaccga
      361 cgacgaggtg gacgttgtcc actgctttca gggcggctac ggcgcggccc agaccatcga
      421 gctgctcgac ttcgacgcca tagggcaaaa ccccaaaccg ttcatcggct actcggacat
      481 caccgcgctt cacaccgcgc tgcgacacta cacggggctg gttacgttct acggaccagg
      541 cctcgccggc gtcaacgaca gggagaccaa gaagttcacg caggagcggc tgttgaaggc
      601 ccttacctca gaagaaccgc tcggcgaggt gccgcgtgac ccgaagaaca cgtacatccg
      661 ctctttcggg ggcgggtcgg tgacggcgcc tatggtgggc gggtgcctgt ggctcctgtg
      721 ccaggctata ggcacgccgt ggcagcccga cttcgacggc aggatcctct tcttcgaaga
      781 cgtggatgcg ccgccctggt atatcgacgg tttcctgaac cagatgcgcc aggccggcgt
      841 gctggacggc gtcgcggggg tggtcgtcgg cgaactggag aaatgcgact ggcgggagga
      901 acggcccgag tttccccaga ccctctccat cgaggacgtg ttagagcgct acatcgagcc
      961 tctgggtgtg ccagccctct atggctaccc tctaggtcac ggcgagcggc tatgcaccat
     1021 ccccctcggc gtgtccgcgg tgttgaacgg agatgacaga acccttacaa tccaggagcc
     1081 agcattactg gaatagacca gcgttgctgg aatcgagggg cgaaggggaa agggaatatg
     1141 cgaaggattt ctgtggtcgt ggtgttggtg gccctggtct cggttcttgc cggcatcgcg
     1201 agcgcggcgg cccaggactc ctcgggggag gatgagaagg tagtcttccg cgtcggggcc
     1261 accaacgata ttgacgggtt caaccccttc aagatcgtcg agatcccctc ctacgaggtc
     1321 atgggcctaa cctatgatct actggtagag acttctccca aggactcgag cccggcgccg
     1381 ggtctggccg aatcttggga gacctcggac gacggtctca cctggacctt ccatctcaac
     1441 aaggacgcca ggtggcacga tggaaaaccc gtgacctccg aggacgtcgc ctacacgtat
     1501 cagcgtattt tggacgaaga gcagggcctg tacatcgacg ccgtcaagca gatcgatagc
     1561 atagagacgc cagacaagca cacggtcgtt ttcacgacca agaaaccatc ggtgcagatg
     1621 ttgaacacgt acgtctacat cttgcccaag cacatctgga aggacgtgcc cgcggacgag
     1681 accaagagct tcaagaacga gccggtgatc ggctccggtc ccttccaggc cgtagaatgg
     1741 agggagggtc agttcgcgcg cctggaggcc aaccccgatt acttccgggg cgctccccac
     1801 gtagacgagg tcatcttcca gttctacgac aacgccgaca caatggtgca ggcgctcaag
     1861 aacggcgagg tggattacat ctacaacatg cccatcaacc tcttcaagtc gctccagaac
     1921 caggagggta tcgagaccaa ctcggcaccc gaccccgggt tcaccgagct cggcttcaac
     1981 ctctacgagc ccacgcccga ggcgatagag gagttcggcg cgccagagaa gaccacgggg
     2041 aacccggcat tgctcgatcc gcgggtgcgt gaggcaatca actgggccat cgacgaggag
     2101 gtccttaccg acaaggtcct acagggcgag ggcgttgccg gcagcacgct cgtaccgccg
     2161 gcgctggcca cctaccacct cgagctcccg gagtccgata ttcagggctt cgacatcgag
     2221 cgctccaggg agctgctggc gcaagccgga tgggaggaca ccaacggcga cggtacggtg
     2281 gacaagggcg gcgaggagct gcagctgcgc ctgttcttgc gcacggagga ttcggactcg
     2341 gccacccagg gcgcgtttct cgaagactgg ttcgagaaag cagggatcgg gctcgagacc
     2401 aaggtcatgg gcgacacggc cctcaccgac gccatctacg ccgcagacta cgacatgttt
     2461 atctggggct ggggctcgga agcggatccc gacttcatcc tctcggtgct cacctgcgac
     2521 cagatcatgg gctggtcgga cacgttctgg tgcaacgaca ggtactccag gatgtacgca
     2581 gagcagaaag agcaactcga cctcgacgag cgggccgcca ccgtaaagga gatgcagcgg
     2641 atcgcctacg aggacaatcc ttacgtgatc ctctactacg acaatcagct cgaagcctgg
     2701 cgcacggaca agttcaaagg ctggagcaag accccgtcgg acgccgaccc cggccaggtg
     2761 gctttcacgt tcacgaccaa gacctacatg gacttgcaac ccctgagcgc gggcggggct
     2821 ggcggcgacg aaagcggtgg cacctcaccg ttgcttttcg tggggctggg cgtcgtcgcg
     2881 atcgcggtcc tcgccggaat cgtcgtcttg atcaggcggg ggagcgcgga ggacagggcc
     2941 tgagccaggc acgttacctg ggcgggaaac tgctgcaggc cgcgggcacg ctgctgttcg
     3001 tgctcgtcct gaacttcttc ctctttcgca tcctgccggg cgaccccgag cgtactttca
     3061 tccggttgca gcgcatctcg ccttcgcaaa tagacgaggt cgagagagag tttgggctgg
     3121 accgcccgct ctgggtacag ttcttcgact accttggcga caccgcgcgg ggcgagctcg
     3181 gcatctccta cgtcttctcc cggccggtcg cggaagttat cggaaccgcg ctgtggccga
     3241 cgatcctgct cgtcggggtg gccacgctgg cggcgacggt gatcgggacg ctcatgggca
     3301 tctggggtgc gtggcgccgt gggagcccca tcgacacctc tctgctcggc ttcgggctcg
     3361 tcacctacgc gatgcccgag ttctggctcg ggatgctcct gatcctcggt ttcgccgtcg
     3421 ctctcggctg gtttccgacc tcgttcatga ccacccccgg gctcgttgac gcctcggcgg
     3481 gcgcgcaact cgtcgacacg atggagcacc tcttcctgcc ggcgctcacc ctgaccctgg
     3541 ccctgatcgg agagtacgcc ctgatcatgc gttccagcgt catggaggcg atgaacgagg
     3601 agtacgtgac gacggcccgc gccaagggga tgcgcgaggc cctgatcttg cgaaaacaca
     3661 tcgtccccaa cgcccttttg cccctcgtga cgctcgccgc gcttaacatc gggttcatcg
     3721 tctccggcgc catcgcggtc gagaccgtct tctcgtggcc aggcctgggc ctgctcacct
     3781 tccgcgccat caacgggggg ccagactacc cactcttgca gggcctcttc ctgctgttct
     3841 cggtggccgt gatcctggcc aacctcctgg cggctctgct acttggcctg ctcgacccca
     3901 ggatcgggcg cgcgtgagta cccaggccac gccaggcgcc cgcttggtcg ggcgcgcgcg
     3961 ccggatccgg gctttcaaat cctcagccag gctcttcgca agaaacaaga tgggcgtggc
     4021 ggggctcacc atcctgaccg ttttcgtcct gctcgccgtc ttcgccgacg tgttagtccc
     4081 acaggccagc ctctccgtga ccaacgcccc gggcaccccg ctagagccac cttcggcgcg
     4141 gttctggctc ggcacggacg agcgggggcg ttcggtactg gatctcgtaa tccaggggtc
     4201 ccagatctcc ttgctcgtcg ggttcctcgc ctccgcggtc tcgatgctca tcggctccct
     4261 ggtagggatc gtcgcagggt acgtcggggg ttggatcgag gttgtcctca tgcgcttcac
     4321 cgactggttc ctggtgatcc ccttcctccc cctggccatc gtgctcgttg ccatactggg
     4381 ccggggactt ttcgttatcg ctttcgttat tgggatcacg acctggccgg gtaccgcgcg
     4441 tgtcgtgcgc gcccaggtac tgacactaaa ggagcgatcc tacgtcgaga gggccagggc
     4501 cctcggcgca agcaatagcc agatcgtaac ccgacacatc ctgcccaacg tgtttcccct
     4561 gatcttcgcc aacaccatcc tgatcgtcgc gatcgcgatc tacaccgagg ctatcctctc
     4621 cttccttggt ctaggcgatc ccttcagcat ctcctggggc agtatcctgg agtccgcctt
     4681 cgcggagggc gccatctcgc tcggcgcctg gtggtacctg gttccgccgg gcctttgcat
     4741 agccctggtg gtgctggcgt tcgcgatgtg cggctacgct ttcgacgaga tactggaccc
     4801 aagactgcga gacagatgac cgaaaccgcc gcgcccacag gaaccctgct ggaggtgaaa
     4861 aacctgcgcg tcctgtacga ggtgcgcgga acgaaggctc cagctgtcga cggcgtaaac
     4921 ctctcgctcc agccgggtga tgccttgggg ctcgcggggg agtctgggtg cggcaagagc
     4981 acgctcgcgc tggcatgcct cggactactg ccgcgcaacg cgacgctcga ggggtctatt
     5041 cgctatcggg agacggaggt gatcggggcc aagccaggac ggctcagggc gctgcgctgg
     5101 gctggcatat ccatggtctt cctgggggcg atgagcgcgt tcaaccccgt caagcgggtg
     5161 ggggaccagg tggtcgaggc tatcctgctg cacgaggacg tctccagggg cgaggcccgc
     5221 cgcagaacgg aggagctctt cgagcaggtc ggcatcccgc cgcgcagggc aggggattat
     5281 ccgcacgagt tctcgggcgg gatgcgccag cgggccatga tcgccatggc cctggcgtgc
     5341 aacccgccgc tggtgatcgc cgacgagccc acgacggcgc tggacgtgat gatacaggcc
     5401 cagatcctga acctcctggc ccgactgcgc gacgagcgga acatagccct gctcgtcatc
     5461 acccatgacc tctcggtcct ggcccagatc gccgaccggg tggccgtcat gtacgccggc
     5521 cgcatcgtcg aagaaggttc cgccgcagaa ctgctcacga gcccccgcca cccctactcg
     5581 aagtctctgg tc
//
LOCUS       DBNGVD010000240         2975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1327341, whole genome shotgun sequence.
ACCESSION   DBNGVD010000240 DBNGVD010000000
VERSION     DBNGVD010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1327341"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC16CM_06685"
     CDS             <1..921
                     /locus_tag="AC16CM_06685"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3139551.1"
                     /translation="SIAGTIGAALAAAMVYGLGEEEIGHAVAISASMGAGIIEANRMG
                     GTVKRIHCGWAAHAGVTAAELAAGGLTGPTTAFEGRFGFLQAYLDDRADAGALTRNLG
                     DRWELSRVFFKPYPANHFTHAGIDAAIRLREDGLEVGEIESIELGVAGPVLRTIAQPE
                     DEKARPKTGYAAQFSGPFTIATALIGGGGLGVSLDDFTDEAVLDRRKLDLASRVRCSA
                     DEECDRIFPDQFPAVLRVDLENGETREARVSHNRGGPENPLSDEELETKFYANATRLL
                     SREGAEALGGALQNLENLSGVDEITRLAQA"
     gene            complement(946..1542)
                     /locus_tag="AC16CM_06690"
     CDS             complement(946..1542)
                     /locus_tag="AC16CM_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3139552.1"
                     /translation="MKVDFYYDLVSPYSYLAYGQIERICRENGAELVLRPMLLGAVHK
                     EAGIKSPIETELKARYQARDIRRWAKYYGVPLMFPDPFPFRTLKTMRAAMFLLKRGEL
                     EAFTEAAFAHYWEEGGAPKGFDEADEEAPISTVATRIGADPGEVLEGAASQEAKQGLK
                     DATSDALRRGVFGAPAFFVGDEMFWGNDRLHFVEAALR"
     gene            complement(1612..>2975)
                     /locus_tag="AC16CM_06695"
     CDS             complement(1612..>2975)
                     /locus_tag="AC16CM_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139553.1"
                     /translation="IVAVALPGIQQDLGFSSTGLQWVISAYTLAFGGFLLLAGRAADL
                     WGRRRMFLVGLAVFTGASLACGLAATPSALVLGRIMQGFGAAIVAPAALSSLTTHFPE
                     GNGRGRALGVGTAAAAGGGATGWLLGGLVAGSLGWEWVFFINLPVGLAGLALAPVLVS
                     ESRDQNAPSRLDVAGAVSVTAGLTLLVYGITRVEEAGFTAPATLASLASSGVLLALFV
                     IVEGWVKNPLVPLGVFRSRVLVGSNLVALVLTAATTPPMLLCVLYVQQVLGFSPAHAG
                     LVFPPFNLAVIGGSLFGPRLVARLGSRATMVCGLILVSAGSATLLGIGPQAGGYLLYL
                     IPAFVLMGTGLGCASVASTASGTAAASGGLQGLASGLLNSAAQIGTVLGLAVLVPLSA
                     ARTEALAHTISHEAALVEGFRLAFVGAAVLAVCGIAVAVLLTGPEQCEAADPSADPEG
                     AVQ"
BASE COUNT          564 a         1034 c          968 g          409 t
ORIGIN      
        1 tccatcgcgg gaaccattgg cgcagccctc gcagcggcca tggtctacgg gctcggggag
       61 gaggagatag gtcacgccgt cgcgatctcg gcgagcatgg gggccggcat tatcgaggca
      121 aaccgcatgg ggggcacagt gaaacgtatc cactgcggct gggccgccca tgcgggcgtt
      181 accgccgcag aactggccgc cggcggtctt accggcccaa ccaccgcttt cgagggccgt
      241 ttcggcttcc tgcaggctta cctcgacgac cgcgcagacg ccggggcgct gacccggaac
      301 ctgggagata ggtgggagct gagccgggtc ttcttcaaac cctacccggc caaccacttc
      361 acccacgccg gcattgacgc ggcgataagg ctccgcgaag atgggctgga ggtcggggag
      421 atcgaaagca tagaactcgg tgtcgcgggc cctgtcctgc gcaccatcgc ccaacccgag
      481 gacgagaagg ccaggccgaa gacgggctac gcggcccagt tctcgggccc ctttaccatc
      541 gcgacggctc tcatcggggg gggaggcctc ggcgtctcac tggacgactt cacggacgag
      601 gccgtgctgg accggaggaa gctggaccta gcctcccgcg tgcgttgctc ggcggacgag
      661 gaatgcgacc ggatcttccc cgaccagttt ccggccgtgc tacgggtcga cctcgagaac
      721 ggcgagacac gcgaggcccg cgtttcgcac aaccgcggag gacccgaaaa ccccctgagc
      781 gacgaggaac ttgagaccaa gttctacgcc aacgcgaccc gactcctctc gagagagggc
      841 gccgaagcgc tcgggggggc gctacagaac ctcgagaatc tgtccggcgt cgacgagatt
      901 acccgcctcg cgcaagccta gcgcgtcggc gctgatcccc cacccttacc gcagcgctgc
      961 ttcgacgaag tgtagcctgt cgttgcccca gaacatctcg tctccgacga agaaggcagg
     1021 ggcgccgaag acgccccgcc gcaacgcatc cgaggtcgcg tccttgagcc cctgtttcgc
     1081 ctcctgactt gcggcgcctt ccaacacctc cccggggtcg gcgccgatcc gggttgccac
     1141 ggtggagatc ggggcttctt cgtcagcctc atcgaaacct ttcggcgccc caccctcttc
     1201 ccagtagtgc gcgaaggccg cctcggtaaa agcctccagt tcgcccctct tcagcaagaa
     1261 catcgcagcc cgcatcgtct tgagggtacg gaaggggaag gggtcgggaa acatcagcgg
     1321 tacaccgtag tatttggccc agcgacggat atccctggcc tggtatctgg ccttgagctc
     1381 cgtctcgata ggggatttta tgcccgcctc tttgtgaacg gccccgagca gcatcgggcg
     1441 cagcacgagc tctgccccgt tctcccggca gatacgttct atttgcccgt acgccaggta
     1501 ggagtaaggg ctgacgagat cgtagtagaa atccaccttc atagggtcgc tcccttcgct
     1561 cacccggctc gccgacccgc gacgattctc cggggattac cggccaggct gctattgtac
     1621 ggcgccctcg gggtctgctg aagggtcggc ggcctcacat tgctcgggcc ccgtcaggag
     1681 aacggcgacg gcgatgccgc aaacggccag tacggctgcc ccgacgaaag ccaacctgaa
     1741 gccctcgacg agcgcggcct cgtgggagat ggtgtgggcg agggcctccg tgcgggcggc
     1801 ggacaggggg accagaacgg cgaggccgag tacggtcccg atctgggccg ccgagttcaa
     1861 gaggccggag gcgaggccct gcaacccgcc gcttgcggcg gcggttcccg aggcggtcga
     1921 ggcgaccgag gcgcatccta gaccggttcc cataagcacg aaagcgggga tcaggtacag
     1981 aaggtacccg ccggcctggg gcccgatccc gagcagggtc gcggagccgg cggagaccag
     2041 gatcaagccg cacaccatcg tggccctcga cccgagccgc gccaccaggc gcgggccgaa
     2101 cagggagccg cctatgaccg cgaggttgaa gggcgggaac acgaggccgg catgggccgg
     2161 cgagaacccg agaacctgct gtacgtagag gacgcagagc agcatgggtg gggtggtggc
     2221 cgcggtgagc acgagagcga cgaggttcga cccgaccagc acccgcgaac gaaacacccc
     2281 aagcggcacg agcgggttct ttacccaccc ctccacgatc acgaagagcg ccagcagcac
     2341 tccggatgag gcgagcgacg ccagggttgc cggcgcggtg aaccccgcct cttccacgcg
     2401 cgtgatcccg taaactagca gcgtcaggcc cgccgtgacg ctgacggccc cggccacgtc
     2461 aagccgggag ggggcgttct ggtcgcggct ctcggagacc agcaccgggg ccagcgccag
     2521 ccccgcgagc ccgacgggca ggttgatgaa gaaaacccac tcccatccca ggcttcccgc
     2581 gacgagacct ccgagcaacc agccggttgc ccccccaccc gccgccgcgg ccgtccccac
     2641 cccaagcgcg cgcccacgac cgttaccctc gggaaagtga gtggtcaggg aagagagcgc
     2701 ggcaggcgcc acgatggccg ccccaaaacc ctgcatgata cggcccagca ctagcgcgga
     2761 cggggtagcc gccagcccac acgccagaga agccccggta aacaccgcga gcccgaccag
     2821 gaacatcctc cttctccccc acaggtctgc cgcccgcccc gccagcaaca ggaaaccccc
     2881 gaaagccagc gtgtaagcgg agatcaccca ctgcaacccg gtactcgaaa acccgaggtc
     2941 ctgctgtatc cccggcaagg ccacggccac gatgg
//
LOCUS       DBNGVD010000241         4957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4971129, whole genome shotgun sequence.
ACCESSION   DBNGVD010000241 DBNGVD010000000
VERSION     DBNGVD010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4971129"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3221)
                     /locus_tag="AC16CM_06700"
     CDS             complement(<1..3221)
                     /locus_tag="AC16CM_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139554.1"
                     /translation="MDSSRIQPSSPRLRGRRSDILPLERTSFIGRGREVAEIERLLSE
                     RQLVTLCGPGGAGKTRLALAVARSLIEAFEGDVWWVELASISIPDLVPRAVATALGVP
                     EAPDLSPAEALVDNLQGRRAFLVLDNCEHLIEACADLADVLLATCPDLTLLATSREPL
                     RVQGETNFMVPILSVPDPGLSLSTGEMADYEAVRLFIERAGAVDSGFALTERNAPAVV
                     RLCDRLNGIPLAIELAAARTRVLSVEQILEKLDDPLGLLSSGGRTVTARHRTLRATLQ
                     WSFELLVEAEKALLYRLSVFVGGWDLEAAEAVGTGDAVEAGLVLDLLSALVDKSLVVV
                     EENGGDLRYGMLEPVRQFGREKLHGSPDEPEVRFRHAEHYLALAERAELELVGPDQGL
                     WLGRLRTEFANLREAHSWSLESGDEEERAWLRLRLPAALWRFWGGQRFEEGKRWLQTA
                     LERDTGEFPATRARALDGLGFILVFQQDYGRAIEALEEAIALYKKLGDLSGAALALAN
                     LGYAALHGGYMDRVPAFVRDGEALMREDLKGHARAYLRVIMATAAMGQGDFDSGVAQV
                     EEGLALCRELGDLRNTSMSLFILGMARLAGGDLDQGASSLEEGAQITRELGDSLGGAY
                     YLLGLGKLAAMRGTPIRAARLWGAAEAQREQLGMALSTFDLAASGYEEDLAAVRSALD
                     RETFESAWAEGRETSSDQAIRDFLEEPAPDEAVAPDLPPAGLPVDDTGGHHNLPASRG
                     GLVGREREMSAIGGTLTSTRLLTLTGPGGCGKTRLALEVARNLVDANTGQYPDGVWLV
                     ELASLTDGTLVPGTVSSALGLRERPDLSATESLVEYLRPRRLLLVLDNCEHLIEACAS
                     LLDTLLGTCEHLDVMATSRETLGIPGEVNWVVPSLTVPDAGSVPYPDDLTRYESVRLF
                     VERARSRAPTFALTLENAAAVASICRKLDGIPLAIELATARMGTLSAEQISERLGDAL
                     GFLTTGDRTRAPRQRTLRATLEWGYELLGEGERGLFGRLSVFAGGWDLEAAEAVGAAE
                     GIGAGEVLDLLGRLVDQSLVVAERETADDRTRYRMLEPIRQYAL"
     gene            3519..4010
                     /locus_tag="AC16CM_06705"
     CDS             3519..4010
                     /locus_tag="AC16CM_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3139555.1"
                     /translation="MGKDRGTPGMDFGVRVAAVVERGGELLLVRHEKPGRDAYWVLPG
                     GRLEPGETIPECAVRELEEETGLAASFSGVLYVGEFLREGRHTIDVVARVTLEGDDEA
                     VLGSDPEVAPDAEPTLREVRWVSVDELRGIELLPDSVKRRLLTDAEAGWMPEEVYLGD
                     SGG"
     gene            4003..4725
                     /locus_tag="AC16CM_06710"
     CDS             4003..4725
                     /locus_tag="AC16CM_06710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3139556.1"
                     /translation="MGEAQGALLSVVIMDAAIAMLVVGGFWLSIFLMRRFGNKVGYSL
                     APLGFSRPEGGTSGGVFLGVFVGVFAITLGFVVNPLSAYVLERLGYSTQSTVQQPFMQ
                     GLSGWVQESPGLAVTAIILVVVVFGPAVEELIFRGVVFNGLYRMGAWFTTRSVGEDVP
                     AMPYGKTIFAFSALVSSVLFALLHLEPVLLPMLLILAVILCYLFRRTGSLLPCIVAHA
                     TFNSFATAIIVLDGLGVLDVPI"
BASE COUNT          804 a         1666 c         1602 g          885 t
ORIGIN      
        1 agcgcgtact gcctgatcgg ttcgagcatc ctgtagcgcg tcctgtcatc cgcggtctct
       61 ctctcggcca cgaccaacga ttgatccacg agccttccga gaagatccag cacctccccg
      121 gcaccgatac cttccgcggc accgacggcc tcggctgcct ccaggtccca gcccccggcg
      181 aaaacggaga gccgcccgaa cagcccacgc tctccctcgc ccaacagttc gtatccccac
      241 tccagggttg cccttagcgt ccgctgcctc ggagcccgcg tccggtctcc cgtcgtcagg
      301 aaccccaagg cgtcccctag tcgctcggag atctgctccg cagagagcgt ccccatcctg
      361 gcggtcgcga gctctatggc aagcggtata ccgtccagct tccggcagat gctcgccacg
      421 gccgccgcgt tctcgagggt cagggcgaag gtcggcgccc tggagcgcgc ccgctccacg
      481 aacagccgca ccgactcgta gcgcgtgagg tcatcggggt acggcacgct ccccgcgtcg
      541 ggcacggtca gggaagggac cacccagttg acctccccgg ggatgccaag cgtctcgcgg
      601 ctggtcgcca tgacgtccag gtgctcgcac gtgccaagga gcgtgtcgag gaggctggcg
      661 caggcttcga tcaggtgttc acagttgtcc aggaccagca acagccgcct ggggcgcaga
      721 tactcgacca gcgattccgt cgccgagaga tccggccgct cgcgcaggcc gagtgcggag
      781 gagaccgtcc ctggcaccag cgtcccatcc gttagcgagg caagctctac gagccagacc
      841 ccgtccgggt attggccggt gttggcgtcc acgaggttac gcgctacctc aagcgcgagg
      901 cgcgtcttgc cgcatccacc gggtcctgtg agcgtcagga gccgtgtcga agtgagcgtg
      961 ccccctatag cggacatctc gcgctctcgt ccgacgaggc cgccccggga ggccggcagg
     1021 ttgtggtggc cgcccgtgtc atcgacggga agccccgctg gaggcaaatc gggtgcgacc
     1081 gcctcatccg gtgccggttc ctccaggaag tctcttatgg cctgatccga tgacgtctcc
     1141 ctcccttcgg cccaggccga ttcgaaggtc tcgcggtcca gcgcggagcg cacggcggcc
     1201 aggtcctctt cgtagcccga ggcggcgagg tcgaatgtgg agagggccat gcccagctgc
     1261 tctctctggg cttctgctgc cccccagagc ctcgcggccc tgatgggcgt gccgcgcatg
     1321 gcggccaact tccccagacc caggaggtag taggcgccgc ccaggctatc tcccagctcc
     1381 cgcgttatct gggcaccttc ctcgagcgac gacgctccct gatcgagatc gccccccgcc
     1441 agcctggcca tgcccaggat gaagagggac atggaggtgt tcctgagatc gcccagctcc
     1501 cggcacaggg ccaggccttc ttcgacctgc gccacccccg aatcgaagtc gccctgcccc
     1561 atcgccgcgg tagccatgat tacgcgcagg taggcgcggg catgaccctt caggtcttcc
     1621 cgcataagcg cctcgccatc ccgcacgaaa gctggcacgc ggtccatgta gcccccgtgc
     1681 aacgcggcgt atccgaggtt cgcgagcgcg agcgccgcgc ccgaaaggtc gccaagcttc
     1741 ttgtagaggg cgatggcctc ttcgagggct tctatcgccc gcccgtagtc ctgttggaaa
     1801 acgaggatga agccgagccc atcgagcgcc cttgccctgg tggcgggaaa ctccccggtg
     1861 tctctctcga gggcggtctg cagccaccgc ttgccctcct cgaaacgctg cccgccccag
     1921 aaccgccaga gcgcggcggg gaggcgcagc ctcagccagg cgcgctcttc ctcgtcaccg
     1981 gactccaggg accaggagtg ggcctccctg aggttcgcga actccgtacg aaggcgcccg
     2041 agccacaagc cctgatccgg cccgaccagc tcgagttcgg cccgctcggc gagggccaga
     2101 tagtgctccg cgtgccgaaa gcggacctcg ggctcgtccg gacttccgtg cagcttctcg
     2161 cgcccgaact gcctgacggg ctcaagcatc ccgtagcgca agtctccccc gttttcctcg
     2221 accacgacca gcgatttgtc cacgagcgcc gacagcaaat ccaggaccag ccccgcttcg
     2281 accgcatccc cggtccccac ggcttccgcg gcctccaggt cccacccccc gacgaacacg
     2341 gagagccggt acaacaacgc cttctcggcc tcgacgagca actcgaagct ccactggagg
     2401 gttgccctta gcgtcctgtg gcgggccgtg accgtgcggc ctcccgagct cagtaggcct
     2461 agaggatcgt caagcttctc gaggatctgt tcgaccgaga gcacccgtgt tcgggccgcg
     2521 gccagctcta tggccaacgg tataccgttc agcctgtcgc atagccgcac caccgccggg
     2581 gcgttccgct cggtcaacgc aaaccctgaa tcgacagcgc cggcacgctc gataaagagc
     2641 cgcaccgcct cgtagtcggc catttctccg gtcgagagcg agagaccggg atcgggcacc
     2701 gagagtatcg gcaccatgaa gttggtctcg ccctgaacgc gcaaaggctc gcgacttgtc
     2761 gccagaagcg tcagatccgg gcatgtcgca agcaggacat cggcgaggtc ggcgcacgcc
     2821 tcgatgaggt gctcgcagtt gtccaggacc agaaaggccc tgcggccctg caggttatcc
     2881 acgagcgcct cggctggaga gagatctggc gcctcgggca cgcccaacgc cgtggctacc
     2941 gcccttggta cgagatccgg gatcgaaatc gatgccagct cgacccacca cacgtcgccc
     3001 tcgaacgctt cgatgaggct cctggccacc gccagggcca ggcgcgtctt gccggcccct
     3061 cccggaccac agagcgtcac aagctgccgc tcggagagca acctctcgat ctcggcgacc
     3121 tccctgccgc gcccgatgaa gctagtgcgt tcgagcggca ggatgtctga tcggcgcccc
     3181 cgaaggcgcg gggaggacgg ttggatcctg gaggaatcca ctacgcgcag cctacgaggg
     3241 ccaggctccg cggaggttct cgacgatggc ggctccggta tacaacgctg atgctctcct
     3301 tccttccgga accaacctta acggactcgg gccgggcgcg tatcccccaa atgggtgatt
     3361 ctcattcctt cctgagacgc gggacggttc acaaaccggg cgggttcgag accagcccct
     3421 acaggggtgt ggggttaccc ggcacgtgtt aggcgaggcg tcgttggacg ggtctggatt
     3481 cacgtaacgt ggtttctcgg cggggttata ctcgctgtat gggcaaagat cgcggaacgc
     3541 cgggcatgga tttcggggtg cgggtggcgg cggtggtaga gcggggagga gagttgctgc
     3601 tggtacgcca cgagaagccc ggtagggatg cctactgggt gctgccgggg ggtaggcttg
     3661 agcctgggga gacgataccg gagtgtgccg ttcgtgagct agaggaggag accggcctcg
     3721 cggcaagctt ctccggggtg ctttacgtgg gcgaattcct gcgcgaggga cgccatacca
     3781 tagatgtcgt ggccagggtg acgctggagg gtgacgacga ggccgttctg gggagcgatc
     3841 ccgaggtcgc gccggatgcc gagcctacct tgagggaggt gcgctgggta agcgttgacg
     3901 agctgcgggg gatcgagctt cttccggatt ccgtgaagag acggctccta accgacgccg
     3961 aagcagggtg gatgcccgaa gaggtctacc tcggtgactc tggtgggtga agcgcagggc
     4021 gccttgctct ccgtggtgat catggacgcc gccatcgcga tgctagtagt cggggggttc
     4081 tggctctcga tcttcctgat gaggcgcttc gggaacaagg ttggctattc cctggcaccc
     4141 ctggggttct cacgtcccga ggggggcacc tctggcgggg tctttctcgg ggtgttcgtc
     4201 ggcgtcttcg ccatcacgct cggctttgtg gtcaacccgc tgagcgcgta cgtcctcgaa
     4261 cggctcggct actccacaca atctacggta cagcaaccgt tcatgcaggg gctctcgggc
     4321 tgggtgcagg agagcccggg cctggccgtg accgccataa tcctggtcgt ggtcgttttc
     4381 gggccggccg tggaggagct aatcttcagg ggggtcgtct tcaacggcct ctaccggatg
     4441 ggagcctggt tcaccacgag gtccgtgggc gaagacgtcc cggccatgcc ttacgggaag
     4501 acaatcttcg cgttctcggc actcgtctct tctgttcttt tcgccctgct gcacctggag
     4561 ccggtgctcc tgcccatgct gctgatactc gcggtgatcc tctgttatct cttccggcgc
     4621 acgggaagcc tgctgccatg tatcgtggcc cacgcgacct tcaattcgtt tgccaccgcc
     4681 attatcgttc ttgatgggtt gggggttctg gacgttccta tctaggggcc gggctttgtt
     4741 gggcggttcg tgaaccgccc ctacatggtt tgttgggtag ccgcaccggc aggtcgggcg
     4801 gggcgtcgta ggagcaggtt cgagacccgc cctccctaga tgcgtgttgc gtggtttgac
     4861 gatagaaagg gggccatccg actccccggc gaaaggaacc tcggatggcc cagccgcagc
     4921 atacctccac cctcgcatcc ctggaagggg cgatcac
//
LOCUS       DBNGVD010000242         4584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2238276, whole genome shotgun sequence.
ACCESSION   DBNGVD010000242 DBNGVD010000000
VERSION     DBNGVD010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2238276"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="AC16CM_06715"
     CDS             complement(<1..968)
                     /locus_tag="AC16CM_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3139557.1"
                     /translation="MKVSRIETLRLGEFPNVLFVLVYTDEGLTGLGETFFGARAVEAY
                     VHETIAPYLLGKDPLRIDWHADQLYGYLGYGSSGVETRGDSAVDIALWDIFGKATGQP
                     LYQLLGGKCRESIRTYNTCAGYRYVREAPRQEVENWGLPEEAAEATEGPYEDLDAFLN
                     RADELAEDLLSQGITGMKIWPFDPYAEASGGHYISDTDLQKALEPFRKIREAVGMQMD
                     VMVEFHSLWDLPTAKRIARATQEFGPYWYEDPIKADALAALAEFASSTHVPVTASETL
                     SGRPAFLQLINKGAAGIVMLDLSWCGGVSEAKKIATMAEACHLPVAP"
     gene            complement(1000..2370)
                     /locus_tag="AC16CM_06720"
     CDS             complement(1000..2370)
                     /locus_tag="AC16CM_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIR3139558.1"
                     /translation="MSSQSAEVGRVGSNKLVYFFGALGGLLFGYDTGVIAGAILFINK
                     DLGLSPFMSGLVVSSLLVGAMIGAAFAGPIADATGRRRLVLVAALVFAVGAIGAALAT
                     NAAMLVLFRVVLGLAVGAASLTVPLYLAEVAPTEIRGAVASLNQLMIVTGILIAYIAN
                     SALAGAGAWRWMIGLAVIPSVLLFLGMLFMPETPRWLVSKDREEEAREVLRRNRDEVA
                     IEAEINDIKRVESQEEGGLRELLAPWVRPALLVGMGLAIFQQIVGINTIIYYAPTTLT
                     NVGFGDSAAIIANAGIGVINVTMTLVAIRFIDRAGRKPLLLAGAAGMALSLAVLGLTS
                     LLLPAPTGVGPVGIITLVCLALFIAAFAVSWGPIVWVMLGEIFPLKVRGSAMAVATVL
                     LWGANFVVSLTFPVLLDALGLGWLFVGYAFIGVAAFFFVRSFVTETKGRSLEEIEADL
                     QERATV"
     gene            complement(2500..3321)
                     /locus_tag="AC16CM_06725"
     CDS             complement(2500..3321)
                     /locus_tag="AC16CM_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3139559.1"
                     /translation="MTGKARDRVINRDSLSTYLEKQIVSGRVAEGSKLPSERAIAGEH
                     GLSRPAVREALRDLAGRGLVEIVPGRGAYVRGPRAGDLVGPLEAFYRRQRVTPRDLVE
                     ARKMIECEAAFLAAGRSGSGDLGAMERALTRFEHSADLVDKTRYDIAFHMSIVRAAHN
                     PVVETMYGSIRGLVVQLMLRSLGDPTVSRAGVPYHREIYEAIRNGDGERARSSMSGHL
                     VIAERLYGDDFDRSLHGVFQRELERLLGPGVTLDELLDLAVETDSDDKEAPWEPA"
     gene            3382..4134
                     /locus_tag="AC16CM_06730"
     CDS             3382..4134
                     /locus_tag="AC16CM_06730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139560.1"
                     /translation="MDGAGVAITGGAGDIGGAMGRELSTLGASVTLIDLKSAEDAEPW
                     LERARGANGEVGYIQADVTDRAAVDEALARIEPLDVAIGNAGIVDSAPFLEITEEQWQ
                     NHLAVNLGGCFNLGQSAARLMVRRERPGRIIFTGSWVQEVPWPEITAYSVSKAGIRML
                     ARQMARELAGHSILVNVIAPGIVRAGLAKRQMETEPQYARRVRHVIPLGEPQTAEQVA
                     RATAFLCSEAADYMTGSTLLVDGGCSLFQFDE"
     gene            4153..>4584
                     /locus_tag="AC16CM_06735"
     CDS             4153..>4584
                     /locus_tag="AC16CM_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HIR3139561.1"
                     /translation="MSTRRYRPQRNWGARVLEYDRAGMRVVFLENEKLRIGLLTDKGT
                     DVIEFNYKPRDVDFVWLAAGGVRNPTSYLSTSPDSLATFLDYYPGGWQEIFPNGGAPS
                     SYLGAQFGQHGEVSNLPWDYEIVEEDEGAVAVSFSVRTQKTP"
BASE COUNT          843 a         1494 c         1450 g          797 t
ORIGIN      
        1 ggggccaccg ggagatggca agcctcggcc atcgtggcta tcttcttcgc ctcgcttacg
       61 ccgccgcacc agctcaggtc gagcatcacg atgccggcgg ctcccttgtt tatcaactgc
      121 aggaaggcgg gacggcccga gagcgtttcg ctcgccgtta ccggtacgtg ggtggatgat
      181 gcgaactcgg cgagggcggc gagggcgtca gcctttatgg gatcctcgta ccagtaaggc
      241 ccgaattcct gggtggcacg tgcgatcctc tttgccgtcg ggaggtccca tagcgagtgg
      301 aactccacca tcacgtccat ctgcataccc accgcctcgc gtatcttgcg gaacggttcg
      361 agggccttct ggagatccgt atcggaaatg tagtggccgc ccgaggcctc cgcgtagggg
      421 tcgaacggcc aaatcttcat gcccgtgatc ccttgcgaga gcaaatcctc ggccagttcg
      481 tcggcccggt tgaggaaagc atcgagatcc tcgtatggcc cctcggttgc ctcggcggcc
      541 tcctcgggca gcccccagtt ttcaacctcc tggcggggcg cctcgcgcac gtaacggtag
      601 cccgcgcagg tgttgtaggt cctgatgctc tcccggcact tcccccccag gagttggtag
      661 aggggctgtc ccgtagcctt gccgaagatg tcccagagcg cgatgtccac ggccgagtcg
      721 cccctcgtct cgaccccgct gctcccgtag ccaaggtagc cgtagagctg gtcggcgtgc
      781 cagtcgatgc gcagggggtc cttcccgagc aggtagggcg cgatcgtctc gtgtacgtag
      841 gcctcgacgg cccgggcgcc gaagaaggtt tcgccgaggc ccgtgaggcc ctcgtcggta
      901 tagacgagga caaagagcac gttcgggaat tccccgagac gaagtgtttc gattcgggac
      961 actttcatgc gggtaccttt cggaacggag ccactcccct tacacagtcg ccctttcctg
     1021 cagatcggct tctatttcct cgaggctgcg tccctttgtc tcggtgacga aggagcgcac
     1081 gaagaagaac gccgccaccc ctatgaaggc gtaccccacg aacagccagc ccaaccccag
     1141 cgcgtcgagc aggaccggga acgtcaagga aacaacgaag ttcgcgcccc acagcaacac
     1201 cgtcgccacc gccatggccg agccccgcac cttgagcgga aagatctcac cgagcatgac
     1261 ccagacgatg ggtccccagc tgaccgcgaa ggcggcgatg aacagggcca ggcacacgag
     1321 cgttatgatg cccacagggc ccacgccggt cggtgcgggc aggagcagcg aggtaagccc
     1381 caggacggcg aggctcaagg ccatgccggc ggcgccggcg agcagtagcg gcttgcgccc
     1441 ggcccggtct atgaagcgta tcgcaaccag ggtcatcgtc acgttgatca cgccgatgcc
     1501 ggcgttggcg atgatggccg ccgagtcgcc gaagcccacg ttcgtgaggg tcgtgggagc
     1561 gtagtagatg atggtgttga tcccgacgat ttgctggaag atggcgagtc ccatgcccac
     1621 caacagagcg ggtctcaccc agggcgccag cagttccctg aggccgcctt cttcctggct
     1681 ctccacgcgc ttgatgtcgt tgatctcggc ctcgatggcc acctcgtccc tgttccgcct
     1741 cagcacctcc ctggcctctt cttcccgatc cttgctcacg agccagcggg gggtctccgg
     1801 catgaagagc atcccgagga agaggagcac ggaggggatc acggccagcc cgatcatcca
     1861 gcgccacgcc ccggccccgg ccagcgccga gttggctatg taggcgatga gtatgcccgt
     1921 gactatcatg agctggttga gggaggcgac cgccccacgg atctcggttg gggctacctc
     1981 cgccaggtac agcggtacgg taagggaagc cgccccgacc gcgagtccca gcacgacccg
     2041 gaaaagcacc agcattgcgg cattggtcgc aagcgccgcg ccgatcgccc ccacggcgaa
     2101 gacgagggcc gccaccagga cgagacggcg ccggccggtg gcgtctgcga tcggccctgc
     2161 gaaggccgcg ccgatcatcg cacccaccaa gagcgagctt accaccagcc cgctcatgaa
     2221 cggcgagagc ccgaggtcct tgttgatgaa caggattgca cccgcgatga ccccggtgtc
     2281 gtacccgaag agcaaccccc caagggcgcc gaagaagtaa accaacttgt tgctgccgac
     2341 cctgcccact tccgcagact ggctagacat gaaccgtcac ctccatactc cctttcccat
     2401 ttcgtgtcct ttctgcgccg ctacaccggc ttccgatggc ttcgttccca acctcctcct
     2461 aagacctgcc gtttcttgtg ctcgccccaa gatggtgcct cacgcgggct cccatggagc
     2521 ctccttgtca tcactatcgg tctcgacagc caggtccagc agctcatcta gcgtcacgcc
     2581 gggaccgagg aggcgctcga gttccctctg gaatacgccg tgcaggcttc ggtcgaagtc
     2641 gtccccgtag agcctctccg cgatcaccag atgaccagac atcgacgagc gggcgcgttc
     2701 tccgtccccg ttgcggatgg cctcgtagat ctcccggtgg tagggcaccc cggcacggga
     2761 gacggtcgga tcccccaaag accgtagcat cagttgcacg accaggcccc tgatcgagcc
     2821 gtacatcgtc tcgaccaccg ggttgtgcgc ggcgcgcacg atactcatgt gaaacgcgat
     2881 atcgtaacgg gtcttgtcta ccaggtcggc gctgtgttcg aagcgcgtga gcgcccgctc
     2941 catggcgccg agatccccgc tcccggacct ccccgccgct agaaaagccg cctcgcactc
     3001 gatcatcttg cgggcctcga caagatcccg cggggtaacg cgctggcgtc ggtagaaggc
     3061 ctctagcggg cccaccagat cacccgctcg cggcccgcgg acgtaggccc cgcgtccggg
     3121 cacgatctcg acgagccccc gacctgccag gtcgcgcagg gcttcgcgca ccgccggccg
     3181 gctcaggccg tgttccccgg cgatagcccg ctccgaaggc agcttcgatc cctccgccac
     3241 gcggcccgag actatctgct tctcgaggta ggtggagagg gagtcccggt tgattactcg
     3301 atctctggct ttaccggtca taccaaaata gtgtacactt tgttactagg tggagtcaac
     3361 ggggaggtgg acgtggatcg gatggacggg gccggcgtag cgatcacggg tggcgccggc
     3421 gacatcggag gtgccatggg aagggaactc tcgaccctgg gtgcttccgt gaccctcatc
     3481 gatctgaagt cggccgaaga cgctgagccg tggttggaga gggccagggg ggccaacggc
     3541 gaggtcggct atatacaagc cgacgttacg gatcgggctg ccgtggacga ggcgctagcg
     3601 cgcatagaac ccctggatgt tgccatcggc aacgcgggca tagtcgactc cgcgccgttt
     3661 ctcgagatca ccgaagagca gtggcagaac caccttgcgg tcaacctggg cggttgcttc
     3721 aatctggggc aatcggccgc acggctcatg gttaggcgcg agaggccggg ccgtattatc
     3781 ttcaccggtt cgtgggtgca ggaggtaccc tggccggaga tcacagcgta ctccgtctcg
     3841 aaggccggaa tccggatgct ggcacgccag atggcgcggg agctagcggg gcacagcata
     3901 ctggtaaacg tgatcgcccc cggcatcgtg agggctggct tggcaaagcg gcaaatggag
     3961 acggaacccc agtacgcgcg ccgcgtgagg cacgtgatac cgctcggaga accgcagacc
     4021 gccgaacagg tcgcaagggc gaccgctttc ctgtgctcgg aggcagcgga ctacatgacc
     4081 ggttcgacgt tgctggtcga tggtggctgc tcgttgttcc agttcgacga gtaatggacg
     4141 cgtgaacgag gcgtgagcac tagaaggtac aggccacaaa ggaactgggg tgcccgcgta
     4201 ctcgagtacg atcgcgccgg catgagggtg gtgttcctgg agaacgagaa gcttcggata
     4261 gggttgctca cggacaaggg caccgacgtg atcgagttca actacaagcc acgggacgtg
     4321 gacttcgtat ggctggccgc aggaggtgta cgcaacccga cctcctacct ctcgacctca
     4381 cctgactcct tagcgacctt cctggattac tacccaggcg ggtggcagga gatcttcccc
     4441 aacggcggcg ccccctcatc ctacctcggg gcccagttcg ggcagcacgg tgaggtgagt
     4501 aaccttccct gggattacga gatcgtcgaa gaggatgagg gcgccgtcgc cgtcagtttc
     4561 tccgtcagaa cccagaaaac gccg
//
LOCUS       DBNGVD010000243         4823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1874079, whole genome shotgun sequence.
ACCESSION   DBNGVD010000243 DBNGVD010000000
VERSION     DBNGVD010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1874079"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="AC16CM_06740"
     CDS             complement(<1..463)
                     /locus_tag="AC16CM_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139562.1"
                     /translation="MIRPSDLERFISDFEDRLAPVERAADEAWWNLATTGTDVAREEF
                     VAAGKAYSDLFSNPDEYGRLRGLYGDLESVESPLLRRRIEVLYRMFEERQGDVETLGR
                     IEELEAEANAVYGNHRSVVGGEKLVENEVRELLRTSDDEGLRREAWEASKSV"
     gene            complement(577..1404)
                     /locus_tag="AC16CM_06745"
     CDS             complement(577..1404)
                     /locus_tag="AC16CM_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030544 - Hsp70 protein binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3139563.1"
                     /translation="MADQINYYEVLGVSREASQAEIRNAYRNLAKERHPDHPGGSTEE
                     FSRLQEANAVLSDPNRRRRHDEALDLAHAADQLAGINFDFDVAEDELSAKRREREAGG
                     PSFGERLRGRFSRKEEEDPYEELRRDARGAGRNQGRRTRGRYDVTEARWYEPHRFDPE
                     PITLKSGAITFVAAFLAFIAVGQIGYWAEGLSAGVFSFLTVLSPFMFILYTLVGLAVA
                     YFAFRTAGYATLAISFVAALVVGAQGPEGLLQFVTMGMIILLVVVYLGNRRESAARR"
     gene            complement(1417..2580)
                     /gene="queG"
                     /locus_tag="AC16CM_06750"
     CDS             complement(1417..2580)
                     /gene="queG"
                     /locus_tag="AC16CM_06750"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3139564.1"
                     /translation="MGEDRTSPDSARRVLEERAREAGFDLVGVASAEPLREGGERLRA
                     WQKEGMAADMGYMHRPVELLSDPKKLQKSARSVVSLGVSYYPGEHPENEGGGRVARYA
                     WGRDYHEVIKARLFRLREELEQELGIRIKARGFTDAVPLLERSAAQHAGLGFFGRNSC
                     LINHDIGSYFFIADLIVDLDLAPDPPGTGTCGRCTRCMDRCPTGAIRAPGVVDARLCI
                     SYLTIENKGEIPRGLREKVGDWAFGCDVCQEVCPYNKTKASRSRWPEFSAESGHGPYL
                     EIAEVLEIRSDEEFGERFAGTPLTRPGRAGLLRNCCVAAGNTRLESAVPALVRALRED
                     PSALVRGHAAWALGEIGGSEAVLREASGAEDDEWCREEIRLALADGTPGAALG"
     gene            2754..3299
                     /locus_tag="AC16CM_06755"
     CDS             2754..3299
                     /locus_tag="AC16CM_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139565.1"
                     /translation="MMLLRLLGIGLAYGFGFMFVKSLFPEPFVLLILQGGQSSEGDLT
                     TLALVYMGVGLIGGLIAAPLFGAAVLPGRARREGRGSRLVLSLAFALLTGLISGILTL
                     VVYATGILPPGGVLDPLVLIRDANHPTGIPLLYAWSIARDLLPAGLAGLFLSPLLGEA
                     LQHLYAAEKAPEQKTYGWEDA"
     gene            complement(3463..3711)
                     /locus_tag="AC16CM_06760"
     CDS             complement(3463..3711)
                     /locus_tag="AC16CM_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139566.1"
                     /translation="MTVFRYGREYFEEGEYVRRVDAKPGDGLDPVVRIVEDLGTVEIP
                     CGKVDCRSNCIEYMVAAKNKRNDYAIAECKVSSLVSII"
     gene            3861..4724
                     /locus_tag="AC16CM_06765"
     CDS             3861..4724
                     /locus_tag="AC16CM_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3139567.1"
                     /translation="MGLTFYIVDVFAEEKYAGNQLAVIRGGADLSDEVLGRITLEMNY
                     SETTFVLSDEEIDGGYDVRIFTPGQEVPFAGHPTLGTAYVIRHEILDEPTDQVTLNLK
                     AGRIPVTFGDVLWMRQLPPTFGPSLDHVSTARALGLEAEELDDRFPVQEVSTGLPAIV
                     VPLRSLAALQRCKIDWERYLQLAGPGKNLYVFCQGSHAEGPGDLSARMFANDLGVAED
                     PATGSAAGCLAGYLVQHRYLVTDSVDVRVEQGYEIDRPSLLYLRATREGDEIAVHVGG
                     KVQMVARGELV"
BASE COUNT          828 a         1684 c         1441 g          870 t
ORIGIN      
        1 cgacggactt ggaggcctcc caggcctccc tgcgcaggcc ctcgtcatca gaggtgcgca
       61 gcagctccct gacctcgttc tcgaccagct tctcgccgcc gaccacgctc ctgtggttgc
      121 cgtagaccgc gtttgcctcg gcctccagct cctcgattcg gcccagggtc tccacgtcac
      181 cctgacgctc ctcgaacatc ctgtacagga cctcgatccg gcgtcgcagg agcgggctct
      241 ccacggattc caggtccccg taaaggccac ggagcctccc gtactcgtcc ggattggaga
      301 acaggtcgct gtacgctttc cccgcggcca cgaactcctc ccgcgccacg tccgtgccag
      361 tggtcgcaag gttccaccag gcctcgtcag ctgcccgctc tacgggcgcc agacggtctt
      421 cgaaatctga gatgaagcgc tccagatcgg agggacgaat cacgaatcag ctccttctag
      481 gtcggtggtg ctcgaaaacg acgagcttcg agtaggaggt atccggctgc aggtttcggg
      541 cttcagggaa ttcacccgat gcctgaaacc tgtggttcag cgccgggcgg cgctctcgcg
      601 tcggttgcct aggtagacca cgacgagcag gatgatcatg cccatggtca cgaactgaag
      661 cagcccctcg ggcccctggg cgccgacgac gagcgcggcc acgaaggaga tcgccagcgt
      721 cgcgtaaccc gccgtcctga acgcgaagta agccaccgcc agcccgacca gcgtgtacag
      781 gatgaacatg aaggggctca gcaccgtcag gaaagagaaa actccagccg atagcccctc
      841 cgcccagtat ccgatctgcc ccaccgcgat aaacgccaga aaggcagcga cgaaggtgat
      901 ggccccactc ttgagtgtta tgggctcggg gtcgaagcgg tggggctcgt accaacgggc
      961 ctcggtgacg tcgtagcgcc cacgggtcct gcgtccctgg ttgcgccccg caccccgtgc
     1021 atccctgcgt agttcctcgt agggatcctc ttcttccttc cggctgaagc gccccctcaa
     1081 gcgctccccg aaggaaggtc cgcccgcctc acgttccctg cgcttggccg agagctcgtc
     1141 ctccgcgacg tcgaagtcga agttgatgcc ggctagctgg tcggcggcgt gggccaggtc
     1201 gagcgcctcg tcgtgcctgc ggcggcggtt cggatcggag aggaccgcgt ttgcctcctg
     1261 cagcctggag aattcctccg tgctgccgcc gggatgatcg gggtggcgct ccttggccag
     1321 gttgcggtaa gcgttcctga tctctgcctg tgaggcctcg cgcgagacgc cgagcacctc
     1381 gtagtagttg atctgatctg ccatcgtttc cctattctaa cccaaggcgg cgcccggggt
     1441 tccgtcggcc agcgccaacc ttatctcttc ccggcaccac tcatcgtcct ccgccccgga
     1501 cgcctcccgt agcaccgctt ccgacccccc gatctcaccc agcgcccacg ccgcgtgccc
     1561 acgcacgagc gccgagggat cttcgcgcag ggcccgaacg agcgcgggta cggcactctc
     1621 cagccttgtg ttcccggcgg caacgcagca gtttcgaaga agcccagccc gtcccggacg
     1681 cgtgaggggg gtcccggcga agcgctcacc gaactcctcg tcggagcgta tctccaggac
     1741 ctctgctatc tccagatagg gaccgtgccc ggattcggcc gagaactcgg gccagcggct
     1801 tctcgacgcc ttggtcttgt tgtaggggca aacttcctgg cacacgtcgc agccgaacgc
     1861 ccagtcaccg accttctcac gcagcccgcg cggtatctca cccttgttct cgatggtgag
     1921 gtaggagatg cacagcctgg cgtccaccac gccgggggcc cttatggccc cggtcggaca
     1981 tcggtccatg cagcgggtac accgtccgca ggtgcccgtg ccgggcgggt caggcgcgag
     2041 gtcgaggtca acgatcaggt ccgcgataaa gaagtacgag ccgatgtcat ggtttatgag
     2101 gcacgagttg cgcccgaaga acccgagccc ggcgtgctgg gccgcggaac gctccaggag
     2161 cggcacagcg tccgtgaagc cgcgggcctt gatcctgatc ccaagctcct gctccagctc
     2221 ctccctcagc cggaacaacc tcgccttgat gacctcgtgg tagtcccgcc cccacgcgta
     2281 ccgcgccacc cttcccccac cctcgttctc gggatgctcc cccgggtagt aggagacccc
     2341 cagggagacc acgctcctcg cgctcttctg cagtttcttc gggtcggata acagctctac
     2401 cggcctgtgc atgtaaccca tgtcggcggc catcccctcc ttctgccacg cccgcagacg
     2461 ctcccctccc tcgcgcaagg gctccgcgct ggccacgccc acgaggtcga agccggcctc
     2521 acgcgcccgc tcctcgagca cacgccgcgc cgagtccggt gatgtccgat cttcgcccat
     2581 gccgcgattc tacccggcca cacgcgaaca ggacgttccg gcgggcccgc cggaacgtcc
     2641 tggcccgtga aagctcttgc tgacgtttct gccgccgcag ggtaaattct tggggcaatg
     2701 ataaaagtgc tctatcagcg tttcatcccc ttcgggccca cggaggaaaa atcatgatgc
     2761 tcttgcggtt gctcggcatc gggcttgcct acggcttcgg cttcatgttt gtgaagtcgc
     2821 tctttcccga gcctttcgtg ctcctgatcc tgcagggagg gcagagcagc gagggcgacc
     2881 tgaccaccct ggcgctcgtc tacatggggg tcgggttgat cggcggcctg atagcggccc
     2941 ccctcttcgg ggcggccgtc cttccgggac gagcccgcag ggaaggccgg ggctcgcgtc
     3001 tggttctcag cctcgccttc gctctgctta cgggcctgat ctccggcatc ctcaccctgg
     3061 tggtctacgc gaccggcatc ctaccgcccg gcggtgtcct cgaccccctg gtcctaataa
     3121 gagacgctaa ccacccgacc gggatcccgc tcctctacgc ctggtccata gcccgagacc
     3181 tgctccccgc aggcctggcc ggcctcttcc tgtcccccct gctcggcgag gcgctccaac
     3241 acctctacgc cgcggagaag gcccctgaac agaagaccta cgggtgggaa gacgcgtagg
     3301 agaggtttca ggccacaggc atcaggcatc aggcttccgg tctcttcggc ggcacccgaa
     3361 ccccctctaa cgcgtaaccc gaaacagggc acatcacccg agtctcgtac agacagagac
     3421 gattcctgca gcacttccta cctgatgcct gtggcctgaa gcctagataa tacttaccag
     3481 cgaggagacc ttgcattcgg caattgcgta gtcgttgcgt ttgttcttgg cggcgaccat
     3541 gtactctatg cagttgctcc ggcagtctac cttgccgcag gggatctcca cggtgccgag
     3601 gtcctcgacg atgcgtacta cggggtcgag gccgtcgccg ggcttcgcgt ccacgcggcg
     3661 cacgtactct ccctcttcga aatattccct accgtaacgg aaaaccgtca tgagcagatt
     3721 gtacacggat ttgacctgga tcacatcgcg gcatacggag caacggtgat gggaatcggg
     3781 gctccccggc gcatctctct tgatgaacgg ctgacggcct gacatgcttg ggtcctgacg
     3841 aaccgtgaga ggagttgcac gtgggcctca ccttctacat cgttgacgtg ttcgccgagg
     3901 agaagtacgc gggcaaccag ctcgccgtga tccgtggcgg agccgacctc tccgacgagg
     3961 tgctcggccg gatcaccctg gagatgaact actccgagac caccttcgtc ctctcggatg
     4021 aggagattga cggcggctac gacgtgcgaa tcttcacgcc tggccaggag gtgcccttcg
     4081 ccggccaccc gacgctcggc acggcgtacg tgatccggca cgagatcctg gacgagccca
     4141 ccgaccaggt aacgctcaac ctgaaggccg gcaggatccc cgtgaccttc ggcgacgtgc
     4201 tctggatgcg ccagctccca cccaccttcg gtccgagcct ggatcacgtc tccaccgccc
     4261 gcgccctcgg cctcgaggcg gaggagttgg acgaccgctt tcccgtgcag gaggtatcga
     4321 ccgggctccc ggccatcgtg gtcccgctga gaagtctggc cgccctgcaa cggtgcaaga
     4381 tagactggga acgctacctg caactggccg gaccggggaa gaacctctac gtcttctgcc
     4441 aggggtcgca cgccgaagga cccggagatc tatccgcccg catgttcgcc aacgacctcg
     4501 gcgtggcgga agacccggcc accggcagcg ccgccggctg cctggccggg tacctggtcc
     4561 aacaccgcta cctcgtaacc gattcggtgg acgtacgcgt cgagcaggga tacgagatag
     4621 atcgtccctc gctcctgtac ctgcgggcga cgagagaagg ggacgagata gccgtccatg
     4681 tcggaggcaa ggtccagatg gtggccaggg gtgagctcgt ctgaggtaga cccatcgttg
     4741 ggttctggat gccccccaga acccccgccg gagtgacccg aaatcaccac aacataacag
     4801 ccgaataaca acacgataac ggt
//
LOCUS       DBNGVD010000244         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_235857, whole genome shotgun sequence.
ACCESSION   DBNGVD010000244 DBNGVD010000000
VERSION     DBNGVD010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_235857"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="AC16CM_06770"
     CDS             <1..298
                     /locus_tag="AC16CM_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3139568.1"
                     /translation="VYQSASSPLRNPLGLPERLVMRFGWTAPGRRIGEALLHLAGVEK
                     PDLSWDLVHPEPWFHNHLSSLHIRGREATLRVEKTTPFNNDEPHLFPIFEHRLA"
     gene            338..739
                     /locus_tag="AC16CM_06775"
     CDS             338..739
                     /locus_tag="AC16CM_06775"
                     /EC_number="4.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-mandelonitrile lyase"
                     /protein_id="HIR3139569.1"
                     /translation="MRIVTATDMQTAPGPEDWFTGAVWRDNAPVEGPPDVAVNRVFFE
                     PGARTNWHTHPEGQILYVVSGTCRAAKEGESPVEVEAGGIVYFAPDERHWHGASPETF
                     MVHMAINIANTTDGGTEWLQPVTDDEYSAFE"
     gene            complement(782..1255)
                     /locus_tag="AC16CM_06780"
     CDS             complement(782..1255)
                     /locus_tag="AC16CM_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3139570.1"
                     /translation="MDESSQEHFDHLRELHFPPERNYLRAHLTLFHKLPGEQEAEISS
                     DLHEICRKQEPLTFSVTGLRLLGRGVAYALSSPQLLALRRELVRGWEPWLGAQDRQGF
                     KPHITVQNKVAPEQARALHQELQATFSPFEVDGLGLSLWRYLGGPWEPAGTYLFG"
     gene            complement(1311..2348)
                     /gene="nrfD"
                     /locus_tag="AC16CM_06785"
     CDS             complement(1311..2348)
                     /gene="nrfD"
                     /locus_tag="AC16CM_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3139571.1"
                     /translation="MGETRRDRIQQGNGRAETTHHPETDAKRKDKNYYGIPPIKRAHW
                     TWQIPLYYWIGGIGAGVQLFSTIAQILGHEDQALTRASRYTVLGTMVVSPVLLIWDLG
                     RPERFYNMLRILKLRSPMSTQSWSVFAFGNLGGLIAVRQAADDGLLGTDNLIAKLARL
                     IPARLLSLLALPIGLNVGSNTGNLIAATSVPIWARNWLFMGPTFLTSGLSTSLSWLSL
                     VLHLTRSGEERTLRALHRAEKATLRVEAGLMAASLARMSRWSKPLFSREVAPLFLGGT
                     LIGGILAPMALLFGRESRSKSLLASVLALGGGLAFRFAIIQAGRKSADDPEAYFTFAS
                     GESAPKPEDKV"
BASE COUNT          444 a          772 c          839 g          403 t
ORIGIN      
        1 cgtctaccag tcagccagct ctccccttcg aaacccgctg gggctcccgg agcgcctcgt
       61 gatgcgcttt ggatggacgg ccccgggacg gcgcatcggc gaggcgctac tacacctcgc
      121 cggcgtcgaa aaaccggacc tgagctggga tctcgtgcac ccggaaccat ggttccacaa
      181 ccacctctcg agcctacaca tccgtggtcg tgaagccacg ctgagggtgg agaagaccac
      241 acccttcaac aacgacgaac cgcatctgtt cccgatattc gaacaccggc tagcgtagaa
      301 agctagcagc tatcagccgc cgacataagg aggcttcttg agaatcgtaa cagccacaga
      361 catgcagact gcgcccggac ccgaggactg gtttaccggt gcggtgtgga gggacaacgc
      421 ccccgttgaa gggccgcccg acgtggccgt gaaccgcgtc ttcttcgagc cgggcgcccg
      481 cacgaactgg cacactcatc ctgagggcca gattctctac gtggttagtg gtacctgccg
      541 ggcggcaaag gagggcgaat cccccgtcga ggtggaggcc ggtggcatcg tctactttgc
      601 cccggacgag agacactggc acggggccag cccggagacg ttcatggtcc acatggccat
      661 aaacatcgcc aacacgaccg acggcggcac ggagtggctg cagcccgtca cggacgatga
      721 gtactccgcc ttcgagtaac ggatcgtggt tgaacagaac cggcgagaca gcccgcggcc
      781 ctcagccaaa aaggtaagtg cccgccggct cccagggacc ccccaggtag cgccacagcg
      841 agagaccgag gccatcgacc tcgaacggcg agaaggtcgc ctgtagttcc tggtgcaggg
      901 ctcgcgcctg ctcgggtgcg accttgttct ggaccgtgat gtggggcttg aaaccctggc
      961 ggtcctgcgc gccgagccac ggctcccatc ctctgacgag ttctcgacgc agcgcaagta
     1021 attgcggtga ggaaagcgcg taagccacgc cgcggcccag caggcggagg cccgtcacgg
     1081 agaaggtgag aggctcctgc ttgcgacaga tctcgtgaag gtcggaggag atctccgcct
     1141 cctgctcgcc cgggagcttg tggaagagcg tcaggtgcgc gcggaggtag ttgcgttctg
     1201 gggggaagtg gagctcgcgt agatggtcga aatgctcttg cgagctctcg tccatccgga
     1261 gcgtcaggat caggggcgcg ggcacgtcgg cgtcgggggc gatctcccgt ttacaccttg
     1321 tcttcgggtt tgggggcgct ctcgccgctg gcgaaggtga agtaggcttc ggggtcgtcc
     1381 gcgctcttgc gtccggcctg gatgatcgcg aaccggaagg cgaggccgcc gcctagcgcg
     1441 agcaccgagg cgaggaggct tttggaacgg ctctccctgc cgaagagcag ggccatcggc
     1501 gcgagtatgc cgccgatgag ggtgccgccc aagaagagcg gggcgacctc ccgggagaag
     1561 agcggcttgc tccaccggct catgcgcgcg agcgaggcgg ccatgagccc ggcctctaca
     1621 cgcagggtag ccttctcggc gcggtgcagg gcgcgcaggg tcctctcttc gcccgagcgt
     1681 gtcaggtgga ggacgagcga gagccaggag agggacgtgg agaggcccga ggtcaggaac
     1741 gtgggaccca taaacagcca gttgcgcgcc cagataggga cggaggtagc ggcgatcagg
     1801 ttccccgtgt tcgagcctac gttgagcccg ataggcagtg ccaggagcga gagaaggcgc
     1861 gcgggtatca gcctcgccag ctttgcgatg aggttgtccg tgccgagtag gccgtcatcg
     1921 gcggcctggc ggacggcgat caggccgcca aggttgccga acgcgaagac cgaccagctc
     1981 tgggtggaca tcggggagcg gagcttcagg atgcgaagca tgttgtagaa gcgctccgga
     2041 cgaccgaggt cccagatcag gagcacgggg ctgacgacca tggtgccgag caccgtgtag
     2101 cggctggcgc gggtgagggc ctggtcttcg tggccgagga tctgggctat ggtggagaac
     2161 agctggaccc ccgctccgat acccccgatc cagtagtaca gtgggatctg ccacgtccag
     2221 tgcgcccgct taatgggcgg gattccgtag tagttcttgt ccttacgctt cgcgtccgtc
     2281 tcgggatggt gcgttgtctc agcccgcccg ttgccctgct ggatccggtc cctccgcgtc
     2341 tcgcccattc gtctaccccc gctccctgaa cgccagcgcc acgaccgccc cgagcaccgc
     2401 cccggctacc atgctcgcga tggtccctcc cttgaccttc tcctgcggca agaaggga
//
LOCUS       DBNGVD010000245         3857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1875340, whole genome shotgun sequence.
ACCESSION   DBNGVD010000245 DBNGVD010000000
VERSION     DBNGVD010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1875340"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..333
                     /locus_tag="AC16CM_06790"
     CDS             124..333
                     /locus_tag="AC16CM_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139572.1"
                     /translation="MNDAVRDRLVTILAAGIAYLISQQVVDRLIDIPDEPGLKDDVIE
                     AVLKGATTATSTILASIIVRRMLKG"
     gene            411..1373
                     /locus_tag="AC16CM_06795"
     CDS             411..1373
                     /locus_tag="AC16CM_06795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3139573.1"
                     /translation="MRVVVIGGSGHVGTFLVPRLVAAGHEVVSVSRGRRDPYQVHPAW
                     RSVEQVTLDREAEEAGGTFGERVLALEPDAVVDMICFMPESAEQLVEALRGRIRHLLH
                     CGTIWVHGHSTQVPATEDLPRFPFGDYGVQKAAIEAYLLDETRKGGIPATILHPGHIV
                     GPGWAPLNPAGNFDLGVFERLARGSELGIPNLGMETVHHVHADDVARAFVLALQNWSV
                     AVGESFHVVSPAALTLRGYAEAVSGWFGQTADLRFVPWERWREEVSEEEAAGTWDHIA
                     HSPNCSIARATELLGYRPRYTSLEAVREALGWLIEHGRIEAEWA"
     gene            1507..>3857
                     /locus_tag="AC16CM_06800"
     CDS             1507..>3857
                     /locus_tag="AC16CM_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3139574.1"
                     /translation="MKQRAWDFREVGRLPSPGDNVAMATRRLEAGTRVSYGDTEFEIP
                     HTILEGHRFAVEPVAEGEDLLSWGLRFGRALRDIAPGDYACNAKILKVLAARHLGFDL
                     PPEPNFSDAELDPYVLDEKRFRSSEQVSLHEEPRTFMGYRRGRGRGVGTRNYVVMMGV
                     TSRLTGFVRALELEMNGVADAYENVDGIVCVAHTEGGEDRKPNNLDLLLRTLSGFMVN
                     PNVGAVLVLDHDGEEAVTNRMLRDYLERHHYPIDDLPHEFMSLEGSFRKDLERAKTVV
                     GGWLGEVDAARRTEVPASELKIGLQCGGSDAFSGVSANPLVAWVGREIVRNGGGVNLA
                     ETDELIGAERYVLENVRDLQTARRFLSCVERFKERVSWHGHTAEDNPSGGNNFRGLYN
                     ISIKSIGAAMKKHPDVRIDQVIEYAQRMDEGGFYFMDSPGNDLESVAGQVAAGANMIF
                     FTTGNGSITNFPFVPTIKFVTTTGRYELLSRDMDVNAGAYLDGTPMDDLGRETFERTL
                     RAASGEKTVGERAGHAQVSIWRDWKQTSGENLDLLENEAEPDGRPLPVKPGVPDVEFS
                     FEAIQSGRGPVVDQIALVMPTSLCSGQISRRIANRLNEGDATRGKVTRFVALPHTEGC
                     GVSAGSAEAIYSRTVLGHLANPSVRFGLLLEHGCEKTHNDYFRNRLEEAGVDPARFGW
                     ASVQLDGGIDSVVAKVEGWFDEVLQDAETLEYESVGPEALRLGLHVSGHVSDEAALGL
                     AGVTRAVVCSGGTVVVPERAAVLSSPAYLESVLGDHPVENTLA"
BASE COUNT          700 a         1184 c         1340 g          633 t
ORIGIN      
        1 cggagcgtgt agctcagacc tccggatctt gtgtggtgaa ggttcgaaac gtgctttcgt
       61 gtttccgagt agtctggcct gggtagttat tcgggcagaa tgcaggagag cgaaggaacg
      121 gttatgaacg acgcggtgcg ggatcggctg gtaaccattc tggcggcggg gatcgcctac
      181 ctgatcagcc agcaggtcgt cgacaggctt atagatatac ctgacgagcc cgggctcaag
      241 gatgatgtca tagaggccgt tctcaagggt gctaccacag cgacctcgac catcctcgcc
      301 tccataatcg tacggcggat gctcaagggc taaaccgcgc gaaacgccat gttcgccagg
      361 ggccacgaac atcaaaaccg cggttctggc ctacgcctgg agggtatctg atgcgcgtcg
      421 tggtgatagg gggcagcggg cacgttggaa ctttcctcgt gccccggctg gtcgctgccg
      481 ggcacgaggt ggtctcggtc agtcgaggca ggagggatcc ctaccaggtc caccccgcct
      541 ggcgctccgt cgagcaggta acgttggatc gcgaggccga agaggccgga ggtaccttcg
      601 gggagcgggt gctcgcgctt gagcccgacg cggtcgtgga catgatctgt tttatgcccg
      661 agagcgccga acagctcgtc gaggcgctgc ggggcaggat caggcatctt ctgcactgcg
      721 ggaccatctg ggtacacggg cacagcacgc aggtgccagc gaccgaggat cttccccgct
      781 ttcctttcgg cgattacggc gtgcagaagg ctgctatcga agcctacctt ctggacgaga
      841 cgcggaaggg gggcataccc gcgacgatcc tgcaccccgg gcacatcgtg ggtcccggct
      901 gggcgccctt gaaccccgcg ggaaacttcg acctcggggt cttcgaacga ctggcaaggg
      961 gaagtgagct gggcatcccc aacctcggga tggagaccgt gcaccacgtc cacgccgacg
     1021 acgtggcccg ggcgttcgtg ctggccctcc agaactggag cgtcgctgtg ggagagagct
     1081 tccacgtggt ctctccagcc gctctgaccc tgcgaggata cgctgaggcg gtctccggct
     1141 ggttcgggca gacggcggac ctccgcttcg tgccgtggga gcggtggcgc gaggaggtct
     1201 ccgaagagga agccgcgggg acctgggacc acatcgccca cagccccaac tgcagcatcg
     1261 ccagagccac tgagctactc ggctacaggc cccgttatac ctcgctcgag gccgtgaggg
     1321 aggcgctggg atggctcatc gagcacggcc ggatagaggc ggaatgggcc tgatccttcc
     1381 agggctggag ggtgatcagg ccgtccgcgg gtaggggacg aacctcccgg ttttaggaat
     1441 ggccgggccg cggggcgcgg tacactcaag acaggaaacg atgccggatc cggtgggagg
     1501 cgggacgtga aacagcgagc gtgggatttt cgggaagtgg ggcgacttcc ttcaccgggc
     1561 gacaacgtgg ctatggcgac ccgcaggctg gaggcgggaa cgcgcgtctc gtacggggac
     1621 accgagttcg agatccccca cacgattctg gagggccacc gcttcgccgt cgagcctgtg
     1681 gcggaggggg aggatctgct ctcctggggc ctccgtttcg gccgtgccct cagggatatt
     1741 gcaccggggg attacgcgtg caacgcgaag atcctgaagg ttctcgcggc gcgtcatctt
     1801 ggcttcgatc tcccccccga accaaacttc agcgacgcgg agctggaccc ctatgtcctt
     1861 gatgagaaac ggttccgttc cagcgagcag gtctcccttc acgaagagcc gcgcacgttc
     1921 atgggctaca ggcgcggacg gggcaggggc gtggggacgc gcaactacgt ggtcatgatg
     1981 ggcgttacct cgaggctcac ggggttcgtg agggcgctgg agctggagat gaacggcgtg
     2041 gcggatgcct acgagaacgt ggacggtata gtctgcgtcg cccatacgga gggcggcgag
     2101 gaccgcaagc cgaacaacct ggacctgctg ctcagaaccc tgagcggctt catggtcaac
     2161 ccgaacgtgg gtgccgtcct cgtcctcgac cacgacggcg aggaggccgt tacgaaccgc
     2221 atgctccggg actacctgga gagacaccac tatccgatag acgacctgcc gcacgagttc
     2281 atgagcctgg aggggagctt caggaaggac ctcgaacggg ccaagacggt cgtgggggga
     2341 tggttgggtg aggtcgacgc ggcacggcgc accgaggtgc cagcctcgga gctcaagatc
     2401 gggctgcagt gcggcgggtc cgatgccttc tccggggtct ccgcgaaccc gctggtggcc
     2461 tgggtaggca gggagatcgt caggaacggc gggggtgtca acctggctga gacggatgag
     2521 ttgatcgggg ccgagcgcta cgttctggag aacgtgagag acctgcagac ggccaggcgt
     2581 ttcctctcct gcgtggagcg gttcaaggag cgcgtctcgt ggcacggtca tacggccgag
     2641 gacaacccct ccgggggtaa caactttcgt gggctctaca acatctccat caagtccata
     2701 ggggcggcca tgaagaagca ccccgacgtg cgcatagacc aggtgatcga gtacgcccag
     2761 aggatggacg agggcggctt ctacttcatg gacagcccgg gcaatgatct ggagagcgtc
     2821 gccgggcagg tcgccgcggg ggcgaacatg atcttcttca ccacgggcaa cggctccatt
     2881 accaacttcc ccttcgtccc aaccatcaag ttcgtgacca ccacgggccg ctacgagctg
     2941 ctctcccggg acatggacgt gaacgctggc gcctacctgg acggcactcc catggacgac
     3001 ctgggccgcg agaccttcga gcgcacgctc cgggcggcct cgggcgagaa gaccgtcggc
     3061 gagcgcgccg ggcacgccca ggtctctatc tggcgcgact ggaaacagac tagcggcgag
     3121 aacctcgacc tgctcgagaa cgaggcggaa ccggacggca ggccgcttcc ggtgaagccc
     3181 ggcgtccccg acgtcgagtt ctccttcgag gccatacaga gcgggcgggg accggtggtg
     3241 gaccagatcg cgctcgtcat gccaacaagc ctctgctccg gccagatctc acgccgcata
     3301 gcgaaccggc tgaacgaggg cgacgctacg aggggcaagg tcacccgctt cgtcgccctg
     3361 ccacacaccg agggctgcgg cgtctccgcg ggctctgcgg aggccatcta ctcccgcacc
     3421 gtgctcggac acctcgccaa cccgagcgtc aggttcggcc tgctcctgga acacggctgc
     3481 gagaagaccc acaacgacta cttccgcaac cgcctcgagg aggcgggcgt ggaccccgcc
     3541 cgtttcggct gggcgagcgt ccagctagac ggcggcatag acagcgtggt ggcgaaggtc
     3601 gagggctggt tcgacgaggt gctgcaggac gccgaaacgc tggagtacga gagcgtcggg
     3661 cccgaagccc tgcggctcgg cctgcacgtc tccggtcacg tctcggatga ggccgcgctc
     3721 ggcctcgcag gggtaacgcg cgccgtggtc tgctcgggcg gcacggtagt cgtgcccgag
     3781 agggccgccg tcctctcatc gcccgcctac ctcgaatcgg tgctaggtga ccatcccgta
     3841 gagaacaccc tggctta
//
LOCUS       DBNGVD010000246         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2422813, whole genome shotgun sequence.
ACCESSION   DBNGVD010000246 DBNGVD010000000
VERSION     DBNGVD010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2422813"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="AC16CM_06805"
     CDS             complement(<1..1548)
                     /locus_tag="AC16CM_06805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139575.1"
                     /translation="MNRLKEVRAGLRRTRETLSNFTPFLRPRRAGLVFALFVLLLETV
                     MSLAQPWPLALIIDYVLTDRKQLPAVVPDLLADEALLLAGIAFLIVLIFTVTRSVASF
                     RRYLLQKLGQQTVFDMREALYAKVHALGLDYHGSKRTGDTITRVTSDVKEVRTMLVDS
                     VVEVFSSITILIGMMVVMLWLDWQLTLLALVTVPFLFLAVSRYRRALVQRMRIVRTRE
                     GAIASVMQEAIIGIRAVKLFAREEDEMERFRKESRESLKASVDSAMIEAKFSTVLGIV
                     GGLGTALVVYFGARQVLSGGLTLGGLTVFVAYLALFLSPLWALSRQVNQIGTSLVSGE
                     RIIDLLSAEPSVEDLPGARPAPEFDGRVAFEDVRFAYDEEAGEILHGVDFDVEAGSRV
                     ALVGVSGAGKTTVTSLIARLYDPQEGRVLIDGEDVKDFTLKSLRDSITFVPQDPLLFR
                     ASVAENISYGRPGASREEIEAVAELAGADRFIRELPEGYDTVLSERGESLSGGQRQRI
                     SIARAMLR"
     gene            1591..2010
                     /locus_tag="AC16CM_06810"
     CDS             1591..2010
                     /locus_tag="AC16CM_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139576.1"
                     /translation="MSNDSTEADVARRVIGALEDLLFKSKISETASQLGIEAAFPRSP
                     KKLLDALHESPPDLLVLDLNSARFGPLELLGQVKSEEALRDVPTVGFLSHVQKDLAVA
                     AREAGCDRIVARSAFTRDLPRILSGDTPDTIDEAGVG"
BASE COUNT          395 a          692 c          684 g          356 t
ORIGIN      
        1 cctgagcatg gcccgcgcga tggagatgcg ctgcctctgc cccccggaga ggctttcacc
       61 acgctcggag agaacggtgt cgtacccttc tggcagctcc cggataaacc ggtcggcccc
      121 ggctagctcg gcgacggcct ctatctcttc ccggctcgcc cctggccgcc cgtacgatat
      181 gttctcggcg acgctggccc tgaacagcag ggggtcctgg ggcacgaacg ttatggagtc
      241 gcgcaacgac ttgagggtga agtccttgac atcctccccg tcgatgagca ccctcccctc
      301 ctgcgggtcg tacaggcggg cgatgaggct ggtcacggtg gtcttgccag cgccgctgac
      361 ccccacgagc gcgaccctgc tcccggcttc cacgtcgaag tccacgccgt gcaggatctc
      421 gccggcctcc tcgtcgtagg cgaagcgcac gtcttcgaag gcgaccctgc cgtcgaactc
      481 gggcgcggga cgcgccccgg gaaggtcctc taccgagggc tccgcgctca ggaggtctat
      541 gatgcgctcg cccgagacga gggaggtgcc gatctggttt acctgcctgc tcaacgccca
      601 aagcggcgag aggaagagcg ccaggtaggc cacgaagacg gtgaggccgc ccagggtgag
      661 gccgccagag aggacctgcc tcgccccgaa gtacacgacg agcgccgtgc cgaggccgcc
      721 gacgatgccg agcaccgtgc tgaacttggc ctctatcatg gcgctgtcga cgctggcctt
      781 gagggactcc cggctctcct tcctgaagcg ctccatctcg tcttcctcgc gggcgaagag
      841 cttgacggcc ctgatgccga tgatggcctc ctgcatgacg ctggcgatgg cgccctcccg
      901 cgtcctgacg atgcgcatcc gctgtacgag cgcccgcctg taccggctga cggccaggaa
      961 gaggaagggc acggtgacaa gcgcgagcaa cgtgagctgc cagtcgagcc agagcatcac
     1021 gaccatcatt ccgatgagga tggtgatcga agagaacacc tcgaccacag aatcgacgag
     1081 catggtgcgc acctctttaa cgtcgctggt gacgcgggtt atggtgtcac cggtgcgctt
     1141 gctgccgtgg tagtcgagcc cgagggcgtg aaccttcgcg tacagggcct cgcgcatatc
     1201 gaagacggtc tgctgcccca acttctggag caggtagcgc cggaacgagg cgacgctcct
     1261 ggttaccgtg aagatcaaga cgataagaaa ggcgatcccg gccaagagca gcgcctcgtc
     1321 ggccaggaga tccggcacca cagccggcaa ctgtttcctg tcggtgagca cgtagtcgat
     1381 aattagcgcg agcggccacg gctgcgcgag gctcatcacg gtctccagta acagcacgaa
     1441 cagcgcgaac acgagcccgg cgcggcgggg tctcaagaac ggtgtaaagt tggatagtgt
     1501 ctctctcgtg cgccgcagtc cggcgcggac ttctttcaat ctattcaccc gtatatgata
     1561 ccgcacccac caggggtaga atgacccaac gtgtccaacg acagcacgga ggccgacgtg
     1621 gcgagaaggg tcattggtgc cctggaagac cttctgttca agagcaagat ctccgagacg
     1681 gcatcccaac ttggcatcga ggcggcgttt ccaagaagcc cgaagaagct gctagacgcc
     1741 ctgcacgagt cccccccgga cctgctggtc ctcgacctga actccgcccg attcgggccg
     1801 ctggagcttc tggggcaggt aaagtccgag gaggctttgc gagacgtccc cacggtcggc
     1861 tttctctcgc acgtacagaa ggacctggcg gtcgccgccc gagaggcggg ctgtgaccgc
     1921 atcgtcgccc gcagcgcctt cacgcgggac ctgccgagaa tactgtcagg ggatactcca
     1981 gacaccatcg acgaggccgg cgttggctga ggggtccgag ggttgggagc ggctcggcag
     2041 cgagaggctc ctctcaaaca ggtacttcac cttgcgctcg gaccggctgc ggctaccgga
     2101 cggggggatc aaagacccat acttcgt
//
LOCUS       DBNGVD010000247         5010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3880202, whole genome shotgun sequence.
ACCESSION   DBNGVD010000247 DBNGVD010000000
VERSION     DBNGVD010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3880202"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC16CM_06815"
     CDS             complement(<1..822)
                     /locus_tag="AC16CM_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3139577.1"
                     /translation="MIPRNSWAVEQSAGRVRFEPPDGVRNDFQRDRDRIIHAKAFRRL
                     MHKTQVFISPDGDHFRTRLTHTLEMMQISRTIARALDLNEDLAEAISLGHDLGHTPFG
                     HTGEQALSRALAPHGRTFHHNEHSLRVVDRLEQGGDGLNLTVEVRDGIRNHTGKVHPS
                     TREGEIVRIADRVAYVNHDVDDALRAGIIRAEDLPEGPISTLGVRMSVRIDTLVRDMI
                     HNSRQKGEIALSDPVYGALMELRSWLFRNVYNKPGSTENQKAAGVVTSLFDYYLEH"
     gene            complement(843..1595)
                     /gene="recO"
                     /locus_tag="AC16CM_06820"
     CDS             complement(843..1595)
                     /gene="recO"
                     /locus_tag="AC16CM_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3139578.1"
                     /translation="MAVYKSKGIVLRSIRYGEADRILDLYTRDAGLISAIAKGIRRTK
                     SRFGARLEPLSCVDFVAYQGRSLDTVTQAETLRSFHGVRENLARFEAAAGMVGSVRAL
                     SGGDEADRRVFNLLYNALDTLEESDSAFESVEASLGLKLSVLAGYAPQLDTCLSCEND
                     LDTAERPLHFAPEHGGVLCAGCRSQTGDAFPLAPGTLESVRALIETPVREAPLQAGPR
                     ENVLRVVRSHVLAHAPGNTLVGRGITGTRRPA"
     gene            complement(1576..2277)
                     /gene="deoC"
                     /locus_tag="AC16CM_06825"
     CDS             complement(1576..2277)
                     /gene="deoC"
                     /locus_tag="AC16CM_06825"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3139579.1"
                     /translation="MRVREFAKTVDHTLLKPDAREEDIVRICEEAARYHFAAVCIPPC
                     YVPLAARQLRGADVKVATVVSFPFGNDATAVKIAAVREAVTRGASELDVVMNISKFLT
                     GQFGYVADELASLTQEVSVVAMNNGLNDVVVKVIIETAYLSDKMKRLATQIVASSGAD
                     FVKTSTGFGPYGATPEDVALLREEAQEGLAVKASGGIRTLEDALDILNAGASRLGMSA
                     SVAVMEEAEGGGLQE"
     gene            complement(2320..3228)
                     /gene="era"
                     /locus_tag="AC16CM_06830"
     CDS             complement(2320..3228)
                     /gene="era"
                     /locus_tag="AC16CM_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3139580.1"
                     /translation="MIGEDADGLKSGFIAVVGRPNVGKSTLVNKLVGQKVSITSPRPQ
                     TTRSPIRGVRNGPGYQAIFVDTPGSQKPRDTLRARMQEGVIDSLSESDAILLLLDAAQ
                     AREGLGSGDRYVARLVAESGTPAIVVINKTDLLRTREEALPIVDAASELADWKEIFQV
                     SAASGTNVEPLMESVVGLLPPGPRYFPEGAVTDYPESLILAEYVREKALGVLREEVPH
                     AVAVEIEDVERKENVTVVYAIIHVERPTQRMIVLGKRGKTIKQIGTEARRDVERLLGT
                     RIYLDLKVKVSPGWRSDRKFLERLGL"
     gene            complement(3225..3614)
                     /locus_tag="AC16CM_06835"
     CDS             complement(3225..3614)
                     /locus_tag="AC16CM_06835"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018018783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3139581.1"
                     /translation="MDRKTLLEAARTASERAYAPYSDFHVGAAILTRAGQVHAGSNIE
                     NASYGLSICAERNAATTMAFADPGDRQIELVAVYSPNASPCFPCGACRQFLREFGCKE
                     VIVEDGSGLRSYPFEEILPNSFGPEDL"
     gene            complement(3626..4369)
                     /locus_tag="AC16CM_06840"
     CDS             complement(3626..4369)
                     /locus_tag="AC16CM_06840"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3139582.1"
                     /translation="MTRRVERAGKGQQGVVRSFEHAYRGMISAVRTQRNMRFHVVVAV
                     LVLLASLLVGASKLELAILVLTIMLVFVTEMVNTAMEFVVDLATREYHPLAKLAKDVS
                     AGAVLVSSVGAVLVGYLVLADDLGPLSLATLESIRRSPAHLTLVSLGVVVLIVLLGKA
                     LTHSPRNFAGGMPSGHAAVAFAGWVAASFIAVAAGAGSYAGLISVISLLMALLVCQSR
                     VESGLHSVYQVVVGAVIGVLVTTAAFQLL"
     gene            complement(4353..4763)
                     /gene="ybeY"
                     /locus_tag="AC16CM_06845"
     CDS             complement(4353..4763)
                     /gene="ybeY"
                     /locus_tag="AC16CM_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3139583.1"
                     /translation="MPVAVVDEVGYERLDVETASHLFSAAFSEMGLDLARIGEVSVAL
                     VDATTIHGLNLRFRNRDEPTDVLSFGVDGPYGEMVGEVVICPSCASPEMGVEELVVHG
                     ALHLAGMDHGEDFESSEMAGVQEAVMGAWRDQEG"
     gene            complement(4790..>5010)
                     /locus_tag="AC16CM_06850"
     CDS             complement(4790..>5010)
                     /locus_tag="AC16CM_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139584.1"
                     /translation="LPKPTPKRIEDVVTDTVRRKLEDGQFDECRLTMHDIHEAGEAIR
                     ESMIGFLGPRIEYPEPTKESRSGKKAAK"
BASE COUNT          851 a         1626 c         1607 g          926 t
ORIGIN      
        1 gtgctccagg taataatcga agaggctggt taccacgccg gcggctttct ggttctccgt
       61 ggagccgggt ttgttgtaca cgtttcggaa gagccaactt ctgagctcca tgagcgcccc
      121 gtaaaccgga tccgacaggg ctatctcacc tttctgtcta gagttatgga tcatgtctcg
      181 aaccaaggtg tcgatcctga cgctcatcct tactcccagg gtagagatcg ggccttccgg
      241 aaggtcctcc gcacggatga tgccggctct cagggcatcg tccacgtcgt ggttgacgta
      301 ggcgacgcgg tcggcgatgc gcacgatctc accctccctg gtagacggat gcacctttcc
      361 cgtatggtta cggatgccgt ccctcacctc cacggtaagg ttcaggccgt ctcccccctg
      421 ttccaggcga tcgaccacgc gcagggagtg ctcgttgtgg tggaaggtac gtccgtgtgg
      481 ggcgagggcc cgggagaggg cctgttcgcc agtatggccg aacggggtat gaccgaggtc
      541 gtgtccgagg gagatcgcct ccgccaggtc ttcgttgagg tcgagggcgc gggcgatggt
      601 gcgtgagatc tgcatcatct ccagcgtgtg ggtgagcctg gtccggaaat ggtctccgtc
      661 gggagagatg aacacctggg tcttgtgcat gagacggcgg aaggctttgg cgtggatgat
      721 gcggtccctg tcgcgctgga agtcgttgcg taccccgtcg ggcggctcga accttacccg
      781 tcctgcgctt tgctctacgg cccaggaatt gcgggggatc accggagccg attcggcagg
      841 tctcacgccg ggcgccgggt tcccgtgatt ccccgtccga cgagggtgtt gccgggagcg
      901 tgggccagaa cgtgggagcg gaccaccctc aggacgttct cgcgcgggcc tgcctgcaat
      961 ggcgcctcgc ggacgggagt ttcgatcagg gccctgacgc tctccagcgt accaggggct
     1021 agcgggaagg cgtcacccgt ctgcgaccgg cagccggcgc acagcacacc cccgtgctcc
     1081 ggggcgaagt ggagcggtcg ctctgccgtg tcaaggtcgt tttcgcaact caggcaggtg
     1141 tcgagctggg gggcgtatcc ggcaaggacc gagagcttca agccaaggga ggcctccacg
     1201 gactcgaagg ccgagtcgct ctcctcgagc gtgtcgaggg cgttgtagag gaggttgaag
     1261 acgcggcggt cggcctcgtc gccgccggag agagcgcgca cgctgccaac catcccggct
     1321 gcggcctcga agcgggcgag gttctccctc acgccgtgga agctacggag ggtctcagcc
     1381 tgcgtcacgg tatccaggga gcggccctgg taggcaacga aatccacgca tgacagaggc
     1441 tccaggcggg ctccgaaacg agatttcgta cgcctgatgc ctttggctat agcggagatc
     1501 aggccggcgt ccctggtata cagatcgagg atacggtccg cctcgccgta gcggatggag
     1561 cggagcacta tccctttact cttgtagacc gccaccctcg gcctcctcca tcaccgccac
     1621 gctggcgctc atgccgagcc gcgaggcccc ggcgttgagg atgtccaggg cgtcttccag
     1681 cgtgcgtatg ccaccggaag ctttcacggc cagcccctcc tgtgcctcct ccctcagtag
     1741 cgccacgtcc tccggcgttg caccgtaagg gccgaagccc gtcgaggtct tgacgaaatc
     1801 cgcgccgctg gaggccacga tctgggtggc cagccgcttc atcttgtccg agaggtaggc
     1861 cgtctcgatg atgaccttga ccacgacgtc gttcagcccg ttgttcatgg cgaccacgct
     1921 gacctcctgg gtgaggctcg cgagctcgtc ggcgacgtag ccgaactgac cggtcagaaa
     1981 tttcgagatg ttcattacca cgtcgagctc ggaggcgccc ctagtgacgg cctccctgac
     2041 ggccgctatc ttgaccgccg tcgcatcatt accgaaggga aaagagacca ccgttgccac
     2101 cttcacgtcc gcgccacgca gctgcctggc ggccagcggc acgtagcagg gtgggatgca
     2161 caccgcggca aagtggtaac gggccgcctc ctcgcagata cggacgatgt cctcttccct
     2221 ggcgtccggc ttcaggaggg tatgatccac cgtcttcgcg aactcgcgta ccctcattgc
     2281 caaatcggtg ctcctctctc ggccctgcgt gcaggggaat tataaaccca accgctctag
     2341 aaacttcctg tcgctccgcc atcccggact aaccttcacc ttgaggtcga gatatatacg
     2401 tgtgccgagc agccgctcga cgtcccggcg ggcttcggta ccgatctgtt tgatcgtctt
     2461 gccacgcttg ccgagcacga tcatgcgctg tgtggggcgt tcgacgtgga tgatggcgta
     2521 gacgacggtc acgttctcct tgcgctcgac gtcctctatc tcgacggcca ccgcgtgtgg
     2581 cacctcttcc ctcagaaccc cgagcgcctt ctcacgcacg tactcggcga ggatcaggga
     2641 ctcagggtag tccgtgacgg ccccttccgg aaaatagcgg gggccagggg gaagtaaccc
     2701 caccacggac tccatcagcg gttccacgtt agtgccgctg gccgcgctga cctggaagat
     2761 ctccttccag tcggcgagct cggaggcggc gtccacgatc ggcagggctt cctcgcgcgt
     2821 gcggaggagg tcggtcttgt tgataacgac gatggcgggt gtgcctgact cggccacgag
     2881 gcgggccacg tagcggtcgc cgctgccgag gccctccctc gcctgtgcgg cgtccaggag
     2941 tagcaagatc gcatcggatt ccgagaggct gtcgattaca ccctcctgca tcctggcgcg
     3001 gagcgtgtcg cgcggcttct gcgagcctgg ggtgtccacg aagatcgcct ggtaccctgg
     3061 gccgtttcgg acgccccgga tcgggctgcg cgtcgtctgg gggcggggtg aggtaatcga
     3121 gaccttctgc ccaacgagtt tgttgaccag cgtgctcttg cccacgttgg gtcgaccgac
     3181 gacggcgatg aagccgctct tcaggccgtc cgcgtcctcg cctatcaaag gtcttctggt
     3241 ccgaacgagt tgggaaggat ctcttcgaat gggtagctcc ggagtcccga cccgtcttct
     3301 acgatgacct ccttgcagcc gaactcacgc aggaactgcc tgcaggcgcc gcatggaaaa
     3361 cacggcgagg cgttcgggct gtacacggcg acgagctcga tctgacggtc cccagggtcg
     3421 gcgaaggcca tggtcgtcgc ggcgttcctc tcggcgcaga tggagaggcc gtaggaggcg
     3481 ttctcgatgt tggagccggc gtgcacctgt cctgccctgg tcaggatggc cgcaccgacg
     3541 tgaaagtcgc tgtacggcgc gtaggcgcgc tccgaggccg tccttgcggc ctccaacaag
     3601 gttttgcggt ccatatcttc tccaatcaca aaagttgaaa ggcggccgtc gtcacgagga
     3661 cgcctataac ggcccccacc acgacctgat agacgctgtg cagcccgctc tcgacccggc
     3721 tctggcacac gagcagggcc atgagcaggg agatcacgga tatgagcccg gcgtaggatc
     3781 cggccccggc cgcgacggcg atgaagcttg ccgcaaccca cccggcgaag gcgaccgcag
     3841 cgtgccccga gggcatgccg ccggcgaagt tgcggggaga atgggtgagg gccttgccga
     3901 gaagcacgat gagcaccacc acaccaagcg ataccagcgt caggtgggcc ggggagcgcc
     3961 tgatgctctc cagggtggcc agcgagagcg gcccgagatc gtctgccaga acgaggtagc
     4021 caacgaggac ggcacccacg ctcgacacga gcacggcccc agccgagacg tctttggcga
     4081 gcttcgccag gggatggtat tccctggtgg ccaggtccac cacaaactcc atcgcggtgt
     4141 tgaccatttc ggtaacgaag acgagcatga tggtcaggac cagaatggcg agctcgagct
     4201 tgctcgcccc cacgagcagg ctggccagaa gcaccagcac ggcgaccacg acgtggaagc
     4261 gcatattccg ctgggtgcgg accgcggaga tcatgccccg gtaagcgtgc tcgaagctgc
     4321 gcacgactcc ctgctggcct ttgcccgccc gctcaaccct cctggtcacg ccacgccccc
     4381 atcacggcct cctggacccc ggccatctcg ctcgactcga aatcctcgcc gtgatccatg
     4441 cccgccaggt gtaacgctcc gtgcaccacc agctcctcca cgcccatctc cgggctcgca
     4501 caggaagggc agatcacgac ctcccccacc atctccccgt agggaccgtc caccccgaag
     4561 ctgagcacgt cggtcggctc gtccctgttc ctaaaccgga ggttgaggcc atggatagta
     4621 gtagcgtcca cgagcgctac ggagacctcg ccgatccggg cgaggtccag acccatctcc
     4681 gagaaggcgg cggagaacag atgggaggcc gtctccacgt ctagccgttc gtagcccacc
     4741 tcgtctacca cggcgacggg catgaatgga cctcccttcg gtcggcggtc tactttgccg
     4801 ccttctttcc gcttcgggat tctttagtgg gttcggggta ttcgatacga ggcccgagga
     4861 acccgatcat ggactcacgg atcgcctccc ccgcctcgtg gatgtcgtgc atggtcaggc
     4921 ggcactcatc gaactggccg tcttcgagct tcctgcgcac ggtgtcggtg accacgtcct
     4981 cgatgcgctt cggggtcggc ttcggcaggg
//
LOCUS       DBNGVD010000248         3192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2058228, whole genome shotgun sequence.
ACCESSION   DBNGVD010000248 DBNGVD010000000
VERSION     DBNGVD010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2058228"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..975)
                     /locus_tag="AC16CM_06855"
     CDS             complement(181..975)
                     /locus_tag="AC16CM_06855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139585.1"
                     /translation="MEANGRLTICQISDIHCGNARFIPDLLDRTIIEINRMNPAVVVV
                     SGDLTDAGYREEFEEAAEYIDRIECEHIMVIPGNHDARNVGYMHFERLFGERFSVLDL
                     EDVILVGVDSSEPDLNDGQVGREHYEFITETFSEAGDKLKIFVVHHHLIPLPGTGRER
                     NIVFDAGDVLQRLIDVGVNLVLSGHKHVPYTWKLEDMIIVNAGTASTLRLRGDTRPCY
                     NIIEVENGKVEVFRKYPFKKREPAVSFEPATQQYLRSKEDTARDYR"
     gene            complement(1047..2627)
                     /locus_tag="AC16CM_06860"
     CDS             complement(1047..2627)
                     /locus_tag="AC16CM_06860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3139586.1"
                     /translation="MPRALMLLAVIVVSLCLSTLLLACSSGPPEGESGGDQGGAGDTP
                     GKQANTGRGNVQEPSGGTDGAEPPAALVPITHLTSTQEGVSMKDLSGARQLAVARGSR
                     EEAGGLVEGAEFRDYDSVGAVIDHVSRTPGALGLVPWDEVGPRVRALPVDGKSLLDPD
                     SEGVEGYPLRPEGGTVPDPEKLRRMVVGGDIVFDRGQYYMVIRRGMGVDFPLDGGYAA
                     VTSRVPEQSSYSETGIIHQFTAERTGGEGAVRDYLSGADLTLANFENPVIRASVYHPD
                     ATTFTGDLRLMPELDQAGIDGVTLGNNHILDAGTEGLRETMGHLDDADIAYAGAGMDL
                     AQARKPMIFDVGGTKIGVLSYMGVPNYDWAWATESTPGTAPLLQNFMEQDIKRLRSKV
                     DVVVVSPHWGVEYMATPEPEQVELAHAAVDAGADVFIGGHAHWPKGIEMYKGTPIYYG
                     VGNFLLDQSWSEETSTGIFADVTLYGDDVIQTRPVPFIMLDRAQPNFLTPRGGGNRAL
                     RNIYAASLGSEFQSSRKR"
     gene            complement(2639..>3192)
                     /locus_tag="AC16CM_06865"
     CDS             complement(2639..>3192)
                     /locus_tag="AC16CM_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139587.1"
                     /translation="DMSFYALVILAVFAVFYVVLRRGLHRTTKAMNPHEGVRHYSPAE
                     ESVNKIEGILKGDRKPPMNPSLDPTTIDVFVSGHTHLPSFSMLEREDAKPCVLVNSGC
                     FLRQLQPISTNLKGPSVFVSKFVLTHVRVFVEDGALRVELWEQPKPASQNMTRLEHLL
                     SLGRRPPQPAPDSKPRLMDSAKL"
BASE COUNT          529 a         1001 c         1049 g          613 t
ORIGIN      
        1 ggagacggcg tcagcataaa gcgggccagg gacgggtcat caccctaggg cagctctcga
       61 gaaaaatggt ccgttcagcg gtgaagggca acaggcatca ggaaaattac ctgatgcctg
      121 ttgcccaagg cccgaagccg cttctcaaac agaaaagctg acagctgata gctattagct
      181 ctaacgatag tcgcgggccg tatcctcctt ggagcgcaag tattgctggg tggcaggctc
      241 gaagctcacc gccggttcac gcttcttgaa cgggtacttg cggaagacct ccaccttccc
      301 gttctcgacc tcgatgatgt tgtagcaggg tcgggtgtcg cccctgaggc gcagcgtcga
      361 ggcggtgccg gcgttgacta tgatcatgtc ctcgagcttc caggtgtagg ggacgtgttt
      421 gtggcccgag aggacgaggt ttacgcccac gtcgatcagc ctctgcagga cgtcgcccgc
      481 atcgaagacg atgttccgtt cacgtcccgt gccagggagg gggatgaggt ggtggtggac
      541 cacgaagatc ttaagtttgt ccccggcctc ggagaaggtt tcggtgatga actcgtagtg
      601 ctcccggccc acctgcccgt cgttcaggtc gggctccgag gagtcgaccc cgaccaggat
      661 gacgtcttcg agatccagga ccgagaaccg ctctccgaag agccgctcga agtgcatgta
      721 gccaacgttc ctggcgtcgt ggttgcccgg tatgaccatg atgtgctcgc actcgatgcg
      781 gtcgatgtac tccgcggcct cctcgaactc ctccctgtac cccgcgtcgg tcaggtcgcc
      841 cgagaccacg accacagccg ggttcatgcg gttgatctcg atgatcgtcc tgtcgagaag
      901 gtcggggata aagcgcgcgt ttccgcagtg gatgtccgag atctggcata tggtcagccg
      961 accgttcgct tccatatctc gctcggatct ccctctccgg ttacctggcg ccccgcacca
     1021 ttatagcctg caacgtttca ggcgcgtcag cgctttctgg aggactggaa ctcggacccg
     1081 agtgaggcgg cgtatatgtt gcgcagggcc ctgtttcctc ctcccctcgg cgtcaggaag
     1141 ttgggctggg cacggtcgag catgatgaag gggacgggtc gggtctggat tacgtcgtcc
     1201 ccgtagagcg tgacgtcggc gaagataccg gtcgaggtct cctctgacca ggactggtcg
     1261 agcaggaagt tgccaacgcc gtagtagatg ggcgtccctt tgtacatttc gattcccttg
     1321 ggccagtgcg cgtggccgcc gataaagacg tcggcccccg cgtcgaccgc ggcgtgcgcc
     1381 agctctacct gctccggctc tggggttgcc atgtactcga cgccccagtg cgggctgacg
     1441 acgacgacat cgaccttcga ccgcagacgt ttgatgtcct gctccatgaa gttctggagg
     1501 agcggcgccg tgcctggggt ggactcggtg gcccaggccc agtcgtagtt gggcacgccc
     1561 atgtaggaga ggacgcctat cttcgtgccc cccacgtcga agatcatggg cttgcgggcc
     1621 tgggcgaggt ccatgccggc tccggcgtag gcgatgtcgg cgtcgtcgag gtgacccatc
     1681 gtctcccgta gcccctcggt cccggcgtcc aggatgtggt tgttgcccag ggtgacgccg
     1741 tctatgcccg cctgatccag ctccggcatc aacctgaggt cgcccgtgaa ggtggtcgcg
     1801 tcggggtggt agacggaggc ccttatgacg gggttctcga agttggcgag cgtgaggtcg
     1861 gcgccggaga ggtagtccct gacggcgcct tcgccaccgg ttcgctcggc ggtgaactgg
     1921 tggatgatcc cggtctccga gtaggagctc tgctcgggca cccggctcgt gacggctgcg
     1981 taacccccgt cgagagggaa gtccaccccc attcccctcc ggatcaccat gtagtactga
     2041 ccgcggtcga agacgatgtc tccgccaacg accatccgcc gcagtttctc ggggtcggga
     2101 acggtcccgc cctccgggcg caggggatag ccttcgacgc cctcggagtc ggggtcgagg
     2161 agcgacttgc cgtcaacggg aagcgccctg accctcggcc ccacctcgtc ccaggggacg
     2221 agacctagcg cccccggcgt ccggctcacg tggtcgatca cggcccccac ggaatcgtag
     2281 tcccggaact ccgccccctc gacgagcccg ccggcctcct cgcgcgatcc ccgcgccacg
     2341 gccagctgcc gcgccccgga gaggtccttc atgctcacac cctcctgggt cgaggtgagg
     2401 tgggtgatcg gaacgagggc ggccggcggc tcggccccat cggtaccccc ggaaggttcc
     2461 tgcacgtttc ctcgcccggt gttcgcctgt ttgccgggcg tatcgccggc ccccccctgg
     2521 tcgccgccag attctccttc cggcggcccg gaagagcagg cgaggagcag ggtggacagg
     2581 cagagagaga cgacgatcac cgccagaagc atcagtgcac ggggcatcct tatctccctc
     2641 atagcttcgc cgagtccatc aggcggggtt tcgagtcggg tgctggttgg ggtgggcgtc
     2701 gtccaaggga gaggaggtgt tcgaggcggg tcatgttctg gctggccggc ttgggctgct
     2761 cccagagctc cacgcggagg gcgccgtctt ccacgaagac gcgcacgtgg gtaaggacga
     2821 acttcgagac gaagaccgac ggtcccttca ggttcgtcga gatgggctgc aactggcgca
     2881 ggaagcaccc cgagttgacc agcacgcagg gcttagcgtc ctccctctcc agcatggaga
     2941 acgagggcag atgcgtgtgt cccgagacga agacgtctat cgtggtgggg tcgagggagg
     3001 ggttcatcgg cggcttcctg tctcccttca ggatcccctc tatcttgttc acggactcct
     3061 cggccgggga ataatggcgg actccctcgt gcgggttcat cgctttcgtg gttctgtgca
     3121 gaccgcgcct cagcacgacg tagaacaccg cgaacacagc caggatcacc agggcgtaga
     3181 agctcatgtc ga
//
LOCUS       DBNGVD010000249         1769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1693770, whole genome shotgun sequence.
ACCESSION   DBNGVD010000249 DBNGVD010000000
VERSION     DBNGVD010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1693770"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="AC16CM_06870"
     CDS             <1..882
                     /locus_tag="AC16CM_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005514967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3139588.1"
                     /translation="GMRFSEAVDTDVTEVEASVAVPRYFAAYEEAFDLPVYRPVKVTV
                     VCDRNGRFRIETNRGNLTPRGVINATGTWETPYVPDYPGADRFRGRQLHTRDYRTAEE
                     FAGRHVIVVGGGISAVQLLDEVSRVTTTTWVTRRPPDFREGPFTEERGRAAVALVEDR
                     VRRGLPPRSVVSVTGLPVTPAVEAMRERGVLNRLPMFEEILDDGVRWPDGTVVRADVI
                     LWCTGFRSTLDHLAPLMLREPGGGISMSGRLATQIAKDPRVHLVGYGPSASTIGANRA
                     GRAAATELMAFLEGKEA"
     gene            887..1105
                     /locus_tag="AC16CM_06875"
     CDS             887..1105
                     /locus_tag="AC16CM_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3139589.1"
                     /translation="MANVANIGREELRARVDGGEHTILLEVLAPRYFRHSHLPGALNL
                     PPGRVREMAPTLLPDKGAEIVLYCWDAA"
     gene            complement(1366..1590)
                     /locus_tag="AC16CM_06880"
     CDS             complement(1366..1590)
                     /locus_tag="AC16CM_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139590.1"
                     /translation="MTTLRVEAKQGEATSTYVTVEQIVRADFYTEGTERNLAYAMVFM
                     TDGNRYRVEGEQAFALETHMTQARISLTPP"
BASE COUNT          288 a          572 c          599 g          310 t
ORIGIN      
        1 gggatgcgct tctccgaggc ggtggacacc gacgtgacgg aggtggaggc gagcgtcgcg
       61 gtaccgaggt atttcgcggc ctacgaggag gccttcgacc tccccgtgta ccggcccgtg
      121 aaggtgacgg tcgtctgcga ccgaaacggt cggttcagga tcgagacgaa ccggggcaac
      181 ctgacgcccc gtggggttat caacgcaacg ggaacctggg agacgcccta cgtcccggac
      241 taccccgggg cggaccgatt caggggcagg caactgcaca cgagggacta ccgaacggcc
      301 gaggagttcg ccggccggca cgtgatcgtg gtcggcggtg gcatctccgc cgttcagttg
      361 ctcgacgagg tctcgcgcgt cacaaccacg acctgggtca cccgcagacc gccggatttc
      421 cgggagggcc cgtttaccga ggagcgcggt cgcgccgccg tggcgctggt cgaagaccgg
      481 gtgcgtcgcg gcctgccccc ccggtcggtg gtctcggtga cggggctgcc cgtcaccccc
      541 gcggtcgagg cgatgcgaga gcggggggtg ctgaaccggc tcccgatgtt cgaggagatc
      601 ctcgacgatg gcgtccgctg gcccgatggc accgtcgtcc gtgcagacgt catcctgtgg
      661 tgcaccggct tccgaagcac gctcgaccac ctggcgccgc tgatgctacg tgagccgggt
      721 ggcggcatca gcatgtccgg caggctcgcc acccagatcg cgaaggaccc gagggttcat
      781 ctggtcggct acggcccctc cgcgtccacc ataggcgcga accgggccgg cagggccgcg
      841 gccacagagc tgatggcgtt tctcgagggg aaggaggcgt agcgcggtgg cgaacgttgc
      901 gaacataggt cgcgaggaac tgagggcgcg ggtagatggg ggagagcaca ccatccttct
      961 cgaagtgttg gcaccccgat acttcaggca ttctcatctg ccaggagcgc taaacctccc
     1021 gccgggcaga gtcagggaga tggcgccgac cttgctccca gataaaggtg ccgagattgt
     1081 gctctattgc tgggacgcag cgtgacccac ctcccggcag gctgcccgtg agctggcggc
     1141 catgggttac accaacgtgc gggactacgt cgagggcaag aaggcgtgga tcgctgcagg
     1201 gctacccgtg gagggcagaa gccggcggct ccgacggccg ggttcatgag gtggcgtggc
     1261 aaagtgagcg cctacggagg tggaccgaag ccccgcaagg gctgtgcttc ctgaaacctg
     1321 atgccttttc caggacgctc ccgccctgcc tgtacgctac ggcgattacg ggggagtaag
     1381 cgagatcctt gcctgcgtca tgtgcgtctc gagggcgaac gcctgctcgc cctccacgcg
     1441 gtaccggttg ccgtccgtca tgaagaccat ggcgtaggca aggtttctct ccgttccttc
     1501 ggtatagaag tccgcgcgca cgatctgctc cacggtcacg tacgtgctgg tggcttcgcc
     1561 ctgttttgcc tcgaccctta gggtggtcat gatcttctcc cctttcctgg ctgcaaccct
     1621 tcctgcctac cacacgagca ttatacgcga cggcccagcc tgccccctac cgccccgtcc
     1681 ttgttcctga agtgaaaccc ggatcggccg gcgggcgtct cacataatgg gtggcggtcc
     1741 ttctctcatc tgccccgcca cccgtattc
//
LOCUS       DBNGVD010000250         5165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1694174, whole genome shotgun sequence.
ACCESSION   DBNGVD010000250 DBNGVD010000000
VERSION     DBNGVD010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1694174"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            526..1803
                     /locus_tag="AC16CM_06885"
     CDS             526..1803
                     /locus_tag="AC16CM_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3139591.1"
                     /translation="MQEVFAISLFVLVLVLIAFELAHRTVLALLGAAVLVAFGVVSQE
                     QAASEFVDWNTIGLLAGMMVIVAILERTGVFEYLAIKSAQWGKARPGRILILLALVTA
                     FLSAFLDNVTTVILMVPVTFLIADALEASPIPFLLTQVMASNVGGASTLIGDPPNILI
                     GSAAELTFADFVANMTPVVLLTLPVVLGILYLMFRRDLGGGDNAEDVIANMDAAGSIR
                     DGVLLRRSLIILALVIVAFFLHGALHLEAATIALFGAAALLLYSGLNVEEVLRDVEWP
                     TLLFFVGLFVLVGGLEVTGFVGSIAEALTGVGGGNSVTTAIVVIWGSGIASGIVDNIP
                     FTATMIPVIQDLAEAQNLSEQQVEPLWWALALGADFGGNLTLIGASANVVVAGMSERA
                     GQRISFLKFMLYGIPVTLASLAVATLYVVVRYY"
     gene            complement(1989..2945)
                     /locus_tag="AC16CM_06890"
     CDS             complement(1989..2945)
                     /locus_tag="AC16CM_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139592.1"
                     /translation="MEPETLTGRQLCYGRCSTAESIYKTPEGGAVIQALYDEALAGLG
                     TGYESLTLRTRFGDTHVLALGSEDAPPVVFLPGGNFLNPTCLGWFLPLAESHRLYAPD
                     IVGQPGRSAQERPSPKGDGHALWVEDVMDGLGLQRAPLVGLSYGAGLAIRVMGLAPER
                     VSRAALVSPSAIATGSVPPMLARVILPMLLYRLRPTRAGLLRAARPLLTEPEEPAFLP
                     AVRQLGAVYRHLRLDAGLPRTATKEELEGFEGPVAVFASEEDAFFPARVVLPRAEEIF
                     PNLVLTERLEGCQHVPSRAAFHRVNERILAFLAADPDGTWPG"
     gene            3412..3813
                     /locus_tag="AC16CM_06895"
                     /pseudo
     CDS             3412..3813
                     /locus_tag="AC16CM_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603311.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
     gene            4015..>5165
                     /locus_tag="AC16CM_06900"
     CDS             4015..>5165
                     /locus_tag="AC16CM_06900"
                     /inference="COORDINATES: protein motif:HMM:NF017495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HIR3139593.1"
                     /translation="MVTILVALTFWLDRRFRLFSFLGTAILVITGAALLVNARIIPPS
                     VPVGDQQTINPFYLFASDYAVPLAIVLLLATADLRSLRLLGRSAILAWVLAAVGTILG
                     TAVAVLLLSGTIGPESWKLGGMYAASYIGGGVNYTAVGEAVDASDTLFATGAAADTIM
                     TNVWMVATALIPALFVRFYASIYDPGRVAEGLAEEEEGTSSEAFWQKKEVSIYDLAYL
                     AAVVFTVLALAELISTAATNLVGFEVPIELWYTTLALLAAFTPVNRLNGGEEIGNFLL
                     HLFFAVLGAGTVLSTLIGQGPIFFLFLVILVTIHALVVFGIGRWRKIEIETLCVASQA
                     TVGGPSTALALAISKRWSALVTPAVLLGVLGYATGNYIGVAMAYLIRSFT"
BASE COUNT          868 a         1730 c         1621 g          946 t
ORIGIN      
        1 accacccgca agggccgcgt catcgtcctc atggaaggac cgcttccagt ccccgatgcg
       61 gacccgctaa ggtcgcagct gaaagagcgg ggcgttgccc cctccgaagt acgagtcgag
      121 ctcgaccccg tgcgtatcgt caccttcgac tgacccggcc gagtcgggcc ggctgacgtt
      181 gatggtagga tcctgaatcc aggtcgtcct cgacgatatg cgggcgcaca acctggatac
      241 cacgagctcg gaggagtaca ccgctccggt gggacgaaag ctgtaagaag ttgctgtacg
      301 ctcttcgaag ccaggccccc gcctcgcgca cgaccgggac gacgggcacg ttgttgtcgc
      361 gcagggcctt caggatctct cgcgcgtggc tctccggcct gacgctcgcg accggtcgcg
      421 tcacgtcctc taacctgatg tggcgtccga atgctggttc tcggtccacc gggtaccctc
      481 gactacctga ctctgccaag cataacctgt actatcctat ggagcatgca ggaagtcttc
      541 gccataagtc tgttcgtgct cgttctggtt ctcatagcct tcgagcttgc ccaccgcacg
      601 gtgctggccc tccttggggc tgcggtgctg gtcgccttcg gcgtcgtaag ccaggagcag
      661 gcggcgagcg agttcgtgga ctggaacacc atcgggctcc tcgccggaat gatggtgatc
      721 gttgcgatcc tggaacgtac cggcgtcttc gagtaccttg ccataaagag cgcccagtgg
      781 ggaaaggcac ggccagggcg tatcctcata ctgctggcgc tcgtaaccgc gttcctctcg
      841 gccttcctgg acaatgttac gaccgttatc ctcatggtcc cggtgacgtt cctgatcgcc
      901 gacgccctcg aggcctcccc gattccgttc ctgctcacgc aggtcatggc gtccaacgtt
      961 gggggggcgt ccaccctcat cggtgacccg ccgaacatcc tgatcgggag cgccgcggag
     1021 ctcaccttcg cggacttcgt cgcaaatatg accccggtag tcctgctcac gctgccggtg
     1081 gttctgggca tcctctacct tatgttccgc agggacctcg ggggcggcga taacgcggag
     1141 gatgtcatcg ccaacatgga cgcggcgggc agcatccgcg acggggtgct gctgcgccgg
     1201 tccctgatca tcctcgccct cgtgatcgtg gccttctttc tgcacggggc gctccacctc
     1261 gaagcagcca ccatagccct cttcggcgcg gcggccctgc tgctctattc aggcctgaac
     1321 gtcgaggagg tcctgcgaga cgtggagtgg cccacgctac tcttcttcgt cggtctattc
     1381 gtcctcgtcg ggggtctgga ggtaaccggc ttcgtgggga gcatcgccga ggcactgacc
     1441 ggggtcggag ggggcaactc cgtgaccacc gctatcgtcg ttatctgggg cagcggcatc
     1501 gcctcgggta tcgtggacaa tattcctttc acggccacca tgatcccggt gatccaggat
     1561 ctggccgagg cgcagaacct ctctgaacag caggtcgaac ccctgtggtg ggcgctcgcc
     1621 cttggcgccg acttcggcgg caacctgacg ctcatcggcg cctcggccaa cgtggtcgtg
     1681 gccggtatga gcgaacgcgc cgggcagagg atctcctttc tcaagttcat gctctacggg
     1741 atccccgtga ccctggcatc cctggccgtg gcgaccctct acgtggtcgt gaggtactac
     1801 tagctccctc cgggtgatcc ttcgaccgag tcctcttcca gcttgcgcag cgtagcagcc
     1861 tcccgaagca gcaggctgcg acgatcaaag ccgcaacgaa agagggccca gacacaagac
     1921 atccttcgct accccgctga agatggccgg gagaacggcc acgatgagaa ggctcgactg
     1981 ggtctcctct accccggcca ggtcccatca gggtccgctg cgaggaaggc gagaattcgc
     2041 tcgttgaccc ggtggaaagc cgccctcgag ggtacgtgct ggcagccttc gaggcgctcg
     2101 gtcaggacga ggttggggaa gatctcctcc gccctcggta gaaccacccg ggcagggaag
     2161 aaggcgtcct cctcggaggc gaacaccgca accggcccct cgaagccctc tagctcttcc
     2221 ttcgtggcgg tccgcggaag cccggcgtcc agccggaggt gacggtagac ggccccgagc
     2281 tgtcggacgg cgggcaagaa ggcgggctcc tcgggctctg tcagcagggg cctcgcggca
     2341 cgcaggagtc ccgcccgcgt tggccgaagg cggtagagga gcataggcaa gatcaccctg
     2401 gcgagcatcg gcgggaccga gccggtagcg atcgcagaag gggagacgag cgcggccctc
     2461 gagacgcgtt ccggggcgag acccatgacc ctgatggcga ggcccgcccc gtaggagagt
     2521 cccaccagcg gcgcgcgctg caaccccagg ccgtccatga catcctcgac ccagagcgca
     2581 tgaccatccc ccttcggcga gggcctctcc tgtgcgctcc tccccggctg gccaacgatg
     2641 tcgggtgcat agaggcggtg gctctcggcc agcggcaaga accacccgag gcaggtcggg
     2701 ttcaggaagt tccccccggg aaggaacacc accggcgggg cgtcctcaga cccgagggcg
     2761 aggacgtggg tatcgccgaa gcgcgtgcgg agggtcaggc tctcgtaccc tgtgcccaga
     2821 ccggccagcg cctcgtcgta gagcgcctgg atcacggccc cgccttcggg cgtcttgtag
     2881 atagactccg ccgtagagca cctcccataa caaagctgac gccccgttag tgtctcaggc
     2941 tccatcggcg acgtccatcc tcggccagga gcacctgcag agcaaatccc ctcgcctgtc
     3001 gagatgctct cccgacagca cgacgcggat ggggacatag tagaggacga cggcctccag
     3061 cgcgtagtcg agggatccac caggtagaac acccgcagcg gcaccagtaa ccgggactcg
     3121 tctagtccca cggactggtc gaggtcagat ccacactccc acgtggggcc agaaagcggc
     3181 gaacagcaca cccgctttgg atgccacaac taccggctcc gcgcctcgta cgcccgcatt
     3241 attgtagagt tcgttatgaa acaagcccag cacggtttct tcggccacca tgacaccatt
     3301 ggcctcgcag gcgaagaaat cgaccgtccc gtgacgggag aaccgctccc cgcctatacc
     3361 gttatccaca gagccccacc actcgatgga cccgggggta cggtggagtg tctgggcact
     3421 gggcccaacc ggagcgcccg gacgaaggac ccgccaggct cgctcgcctg ggagtcggcg
     3481 ctgctcgaga cacacgtcga cgtggtgggt gacctggagc tgcgcctcga cgccagcgcc
     3541 accgcggccg ataccgcctg gatcgccctg ctgcaagacg tcgcccccga tggcaccgtc
     3601 acagaggtca ccgccggctg gctgcaggcg agtctgcaca cggtcgatga gcaggccacc
     3661 gcatccgcct gctgctgtgc agcgacgacc agcccaggga gatacccaac catcattggc
     3721 ttccgacacg cgtccgtggg cacgagcagc cgcaataccg tctactccag ttcgcggcta
     3781 ctcttgccgg tgatctcgtc agccgcgccc tagccgcctc gtggaagcgg cggtacgtcg
     3841 cttcgctgtt ttcgggacgc agggtccgac cctacgccgc gtagatcttg acgcacggcc
     3901 gggggcctga ctatgatgct tcccaggtaa gggggagtgg agggttcgag cgcaggtggg
     3961 gggaacgtct tgggtggaca ggcgcttgtc actagcccgt ttggggttgg ggctgtggtc
     4021 acgattcttg tcgccctcac cttctggctg gaccgcaggt ttcggctatt ctcctttctg
     4081 ggcacggcga tcctggtcat taccggcgcc gccctcctgg tcaacgcccg catcatcccg
     4141 ccttcggtac ccgttgggga tcagcagacg ataaatccct tctacctctt cgccagcgac
     4201 tacgccgtgc ccctggccat agtcttgctg cttgcgaccg ccgacctgag gagcctgcgc
     4261 ctacttggta gatccgccat cctggcctgg gtactggccg ccgtcgggac tattctcggc
     4321 acggcggtgg cggtgctgct tctctcgggc accatagggc ccgagtcctg gaagctcggc
     4381 gggatgtacg ccgccagcta cattgggggc ggcgtcaact acacggctgt tggtgaggcg
     4441 gtagacgcct ccgacaccct gttcgccacg ggtgccgccg cagacaccat catgaccaac
     4501 gtctggatgg tcgcgacggc cctgatcccg gcgctcttcg tccggttcta cgcttccatc
     4561 tacgatccgg gtagggtcgc cgaggggctc gccgaagaag aggaaggaac ctcctccgag
     4621 gccttctggc agaagaagga ggtctcgatc tacgacctcg cctacctcgc cgccgtggtc
     4681 ttcaccgtgc ttgcccttgc cgagctgatc tcgaccgcgg caacgaacct ggtcggcttc
     4741 gaggtgccga tagagctgtg gtacaccacg cttgccctcc tggccgcctt cacgcccgtg
     4801 aaccggctga acggtgggga agagatagga aacttcctcc tgcacctgtt cttcgccgtc
     4861 ctcggggccg ggacggttct atcgaccctg atcggccagg gcccaatctt cttcctgttc
     4921 ctggtcatcc tggtgactat ccacgccctt gtggtgttcg ggatcggacg ctggagaaag
     4981 atcgagatcg agacgctgtg cgttgccagc caggccaccg tcgggggacc ctctacggcg
     5041 ttggcactcg cgatctccaa gagatggagc gccctcgtca cgcccgcggt gctccttggc
     5101 gtccttggct acgctacggg caactacata ggggtcgcaa tggcttacct gatacggtcg
     5161 ttcac
//
LOCUS       DBNGVD010000251         3596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4791108, whole genome shotgun sequence.
ACCESSION   DBNGVD010000251 DBNGVD010000000
VERSION     DBNGVD010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4791108"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..238)
                     /locus_tag="AC16CM_06905"
     CDS             complement(47..238)
                     /locus_tag="AC16CM_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139594.1"
                     /translation="MGPEIWQFLLVLLGFAVVAVLWLLIVYLPGRTLERWEDQRPGHG
                     KGGSSSDPGNPDGPLKPGS"
     gene            complement(358..1266)
                     /locus_tag="AC16CM_06910"
     CDS             complement(358..1266)
                     /locus_tag="AC16CM_06910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3139595.1"
                     /translation="MDKNLWKSERVPGEVEISIGRAGEGGDPLVCLHGITAQHRAFNA
                     TARHLAPSHGLVGVDLRGRGDSDKPESGYGLATHAADVIRVLDHLGLEDAVLVGHSMG
                     AFVALRAALASPERVRALVLLDGGWPRVESSPGEMTGEEKQEARVIEEGLARAFRRLD
                     MVFDSPAAYLDFWFPDRGLTMEDLPPDLADYYLYDLERVDGGYNPKASRVAAEEDSPS
                     VASTGPTAVEMRDVACPVALVRAEQGFFPGSDPLVSDQARDVMVGSLDIREEMLVRGA
                     NHYTLLWPEYTGVWVPRVFDPPFWER"
     gene            1323..2258
                     /locus_tag="AC16CM_06915"
                     /pseudo
     CDS             1323..2258
                     /locus_tag="AC16CM_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
     gene            2324..3484
                     /locus_tag="AC16CM_06920"
     CDS             2324..3484
                     /locus_tag="AC16CM_06920"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR3139596.1"
                     /translation="MKHSESGFSTRAIHAGQEPDPATGATIVPIYQTSTYTQEAPGQN
                     KGYEYSRSGNPTRAALEECVAALEGAEHGLAFASGLAATTAVMSLLSPGDHIVAGDDL
                     YGGTYRLFGKVLSESGGLDFTYADTTDPASVEEALRPETKMLWIETPTNPMLTLSDIA
                     GLSEMAHERGAVVAVDNTFASPYFQNPLALGAEIVVHSTTKYMGGHSDVIGGAVATSN
                     EKFYEEIKFYQNAAGAVPGAFDAWIVLRGLKTLAVRMRQHEENALAVARFLQDHPQVE
                     TVNYPGLPSHPQHELAKKQMSGFSGMVSFTLKGGTEAAYAAVQKTRVFHFAESLGGVE
                     SLITHPASMTHAAIPRDQREARGVTDGLLRLSVGIEDARDLISDLDQAISAG"
BASE COUNT          653 a         1202 c         1202 g          539 t
ORIGIN      
        1 gaggatcacc agatggtcga gccgggtcag cacggcatgc cgggtgctag gaccccggct
       61 tcaggggccc gtccgggttg ccgggatcgg acgacgatcc gcccttgccg tggccggggc
      121 gctgatcctc ccaccgctcc agggtgcggc ccgggagata cacgatgagg agccacagca
      181 cggcgaccac cgcgaacccc agcaagacaa gcaagaactg ccagatctcc ggccccaaga
      241 atactcatct ccctcgtctt acggtgtttg tactggctac gggtacatta tcgtacacga
      301 cggctgctta cgtcgtgaat gctttgccct ggtctctcta cccggcatcg tttcggctca
      361 acgctcccag aagggcgggt cgaagaccct cggaacccag accccggtgt actcgggcca
      421 gagcagcgtg tagtggttgg cgccccggac aagcatctct tcgcggatgt cgagggaccc
      481 gaccataacg tcgcgggcct ggtccgacac gagcgggtcg ctgccgggga agaagccctg
      541 ctcggccctg acgagcgcca ccgggcaggc cacatcccgc atctccacgg cggtaggacc
      601 ggtggaggca accgaggggg agtcctcttc agccgccacg cgggaggcct tgggattgta
      661 gccgccatcg acccgttcga ggtcgtagag gtagtagtcg gcgaggtcgg gcggcaggtc
      721 ttccatggtc aggcccctgt cggggaacca gaaatcgagg taggccgcgg ggctatcgaa
      781 gaccatatcg aggcgccgga aggccctggc gagcccctcc tctataaccc tggcttcctg
      841 cttctcctcc ccggtcatct cacccggcga gctctcgacg cggggccagc cgccgtcgag
      901 caggacgagg gcccgcacgc gctcgggtga ggcgagcgcg gcccgcaggg ctacgaaggc
      961 gcccatggaa tgccccacga ggacggcgtc ctccagaccg aggtggtcca ggacgcggat
     1021 cacgtcggcg gcgtgcgtcg ccaggccgta gcccgactcg ggcttgtcgg agtcgccacg
     1081 accacgcagg tcgacgccga cgagaccgtg ggatggtgcg aggtgacggg ccgtcgcgtt
     1141 gaaggccctg tgctgggccg tgatgccgtg gaggcatacg agggggtcac cgccctctcc
     1201 ggcgcggccg atcgagatct ccacctcgcc ggggacacgc tcgctcttcc agaggttctt
     1261 atccatgggc cgcgattata cgccagggca ttcggagaag ccggtggtgg taatctacct
     1321 tcatggacta ctacgagaac gtgctggggg ctgtcggcaa cacaccgctc gtcaggctcc
     1381 ggaagatcga gcacgacgaa ggcctggcgg ccaccgtcct agccaagctc gagtacatga
     1441 acccggccgg gtcggtgaaa gacaggccgg ccatcaagat gctggaggtg gccgagaggg
     1501 agggtctcct gaaaccgggc gggaccgtgg tcgagcccac gagcggcaac acgggcgccg
     1561 gcctggccct ggccgccgcg gtcaggggct acaggtgcat ctgcgtgatg ccggacaagg
     1621 tgagccagga gaagcgggac ctcctccggg ccatgggcgc cgaggtcgtc gtaacgccta
     1681 cgaccccgcc agaggatccc gagggctact acgccgtcgc cgagaggctg gcggaggaga
     1741 tccccggcgg cttcaagccg ggccagtacg agaacccgtc caaccccctt gcccactacg
     1801 agacgacggg acccgagctc tggcggcaga ccgacggcct cataacccac ttcgtcgccg
     1861 ggatgggcac ctgcgggacc atcacgggca cgggccggta cctgaaggag cagaacccgg
     1921 acgtgagggt cgtgggggtc gaccccgagg ggtccatctt ctccgaccct ggcaacatcc
     1981 accagtacgc cgtggagggc gtcggggagg acttctaccc gggaaaccac gacgcgtccg
     2041 tggtcgacga ggtgatccag gtcgacgacc gcgaatcgtt tctgatgacc agacgcctga
     2101 tgtccgaaga gggactcttc gtcggcggct cgtgcgggat ggcggcggtc ggggcggtca
     2161 gggccgcgaa ggacctgccc gagagcgccg tgctcgtcgt gctgctgccc gacacggggc
     2221 gtaactacgt ctccaaggtc ttcgacgacg actggatgct cgttaattcc ggcgcctcgc
     2281 gggaagacct gggcaaaccc taccgcgtct tcggggagga atcttgaagc attccgagag
     2341 cgggttctca acccgcgcca tacacgccgg ccaggagcca gacccggcca ccggcgccac
     2401 catcgtcccc atctaccaga cctccaccta cacgcaggag gcaccaggac agaacaaggg
     2461 ctacgagtac agcaggtcgg gcaacccgac gcgcgctgcc ctcgaggaat gcgtcgccgc
     2521 cctcgaaggg gccgaacacg ggctcgcctt cgcctcgggg ctggcggcaa cgaccgcggt
     2581 gatgagcctt ctctcgccag gcgaccacat cgtcgccggg gacgacctct acggcgggac
     2641 gtaccggctc ttcggcaaag tgctctccga gtccggcggg ctggacttca cgtacgccga
     2701 caccacggac cctgcctccg tcgaggaggc gttaaggccg gagacgaaga tgctctggat
     2761 cgagacgccg accaacccga tgctgacgct ctcggacata gccgggctct cggagatggc
     2821 ccacgaacgg ggcgcggtcg tggccgtcga caacacgttc gcctcgccct acttccagaa
     2881 cccgctcgcg cttggggccg agatagtggt ccattccacg accaagtaca tgggcggaca
     2941 ctccgacgtg atcggtgggg ccgtggcgac ctccaacgag aagttctacg aggagatcaa
     3001 gttctaccag aacgccgccg gcgcggtgcc tggcgcgttc gacgcctgga tcgtgctgcg
     3061 cggcctcaag accctcgccg tccgcatgcg ccagcacgag gagaacgccc tggccgtggc
     3121 acgattcctg caagaccacc cgcaggtcga gaccgtgaac tacccgggcc tgccgagcca
     3181 cccgcagcac gagctggcca aaaaacagat gagcggcttc tccggcatgg tctccttcac
     3241 gctgaaaggc ggtaccgagg cggcctacgc tgcggtgcag aagacgcggg tcttccactt
     3301 cgccgagagc ctcggcgggg tcgagtccct cataacccat ccggcctcca tgacccacgc
     3361 cgcgatcccc agagatcagc gcgaggccag gggcgtcaca gacggcctgc tccggctctc
     3421 cgtcggcatc gaggacgcgc gggatctgat ctccgacctc gaccaggcca taagcgccgg
     3481 ctagccttgc atggcgagta caagacccct gcggtcaagc tcgtcgtggt ggacttcggg
     3541 gtcgaggatg gcaacttgtc gaacgtcttg gtaagcggcg acttctttct cgaacc
//
LOCUS       DBNGVD010000252         6279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_966409, whole genome shotgun sequence.
ACCESSION   DBNGVD010000252 DBNGVD010000000
VERSION     DBNGVD010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_966409"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1644)
                     /locus_tag="AC16CM_06925"
     CDS             complement(373..1644)
                     /locus_tag="AC16CM_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3139597.1"
                     /translation="MKFLSRSRVTQSAGAGSVYIAVSFGVSWVLTFAFQGLSTRFLGV
                     QNYAPLALLWSATFLTVQVLWVGATQTLGRYVAEREAKGEDWEPVVVSVRRWQIGLSG
                     VFLLGALLASPALTNLFGSVWLTVAFVAAVLLYAPEYFRRGVFNGHRQPLRLGVQIVA
                     ESAGRVLIAAVLLVVGWGVFGPAIAIVLAPLIGVLAVRPARVPPPKGEGQPFSTGHAL
                     QFAAPVLACMAFAQALMNGGPILADLIGGTRAQVSLFAAALIFTRIPQYVLSPAIGAL
                     LPRASRVLSTDGRAAFERFLLRALGVVGLVGVFMVGGTWLLGGWGLRLFAGEEFVVER
                     AVLVALAAMAAFYLLSETVSQGLFALGRGRIAALGWFVGLLASAVCLVTLGVGVVERV
                     SYSLALGTLAATVMQTLLYLASRGRADGTPR"
     gene            complement(1786..2832)
                     /locus_tag="AC16CM_06930"
     CDS             complement(1786..2832)
                     /locus_tag="AC16CM_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3139598.1"
                     /translation="MVEHVSIAGVVIALLFAAYGAYRYRGGGSRLDLLLSLLIAIGVA
                     LVSVVPQFFGIFTRLLGLEDRGFALLSIAVLLLFGLFLNLLARVREATRRNGEIVTGL
                     AVKEYSERYLSSEERVEGHPGEVLIIVPAYDEEGGIRDVLRRIPDEVLGHQVKTVVVD
                     DGSADRTADFARAEGVPVVSHVVNRGQGDALRTGFAIARAEKALVVINLDADGQYKPE
                     ELGRLVGPIIEDRADFVLGSRFMGYYQEAGSVRHIGVVFFSRLISLLTGVKVSDCTNG
                     YRAIRVSELHKLNLREDRFNANEIILEGLKSKLRFEQVPVSMMSRAAGETKKPPKLAY
                     PLGVFRVIISTWLR"
     gene            complement(2836..4035)
                     /locus_tag="AC16CM_06935"
     CDS             complement(2836..4035)
                     /locus_tag="AC16CM_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008185904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3139599.1"
                     /translation="MKVLVLGGDGFCGWPTSLHLSAQGYEVAIVDNLSRRKIDVELEC
                     ESLTPIRPMGERLRVWEDLTGNRIDFHNFNVAENYQKLLDLLLEWRPDAVIHFAEQRA
                     APYSMKSSYHKRYTVNNNLNATNNLLAAITESGLDTHVVHLGTMGVYGYGTAGMQIPE
                     GYLTVKVETEEGEMVEQEILYPPNPGSIYHMTKTQDQLLFAYYNKNDLTRVTDLHQGI
                     VWGTQTAETRLDERLINRFDYDGDYGTVLNRFLMQAAVGYPLTVHGTGGQTRAFIHIQ
                     DTVRCIQLALENPPEKGDRVNVYNQMTETHRLRDLATLVSERTGAEIDYVQNPRNEAD
                     ENDLHVRNDRFLDLGLQPTTLSEGLLEEVTEIARKYADRCDTSKIPATSLWRPREEVV
                     PQQDGHR"
     gene            4399..5958
                     /locus_tag="AC16CM_06940"
     CDS             4399..5958
                     /locus_tag="AC16CM_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR3139600.1"
                     /translation="MAAVSLAGIGVFLFVFALYVMTLSPTVLYLQDPKLLDAVMVQMQ
                     VAVLGMTHPTGYPTYLMLTHLFTYLPVGDPAYRVNLGSAFYAALAVVAVYLTGLLLTR
                     RVVAAVAGALAFGLGAAIWSQAVIAEVYTLNALLVAATVGVLLLWRLRRMDRYLLLSA
                     FLMGLCLTNHLTSGLLLPAGVLLVALVDRAKLKDVRLLLKGAGLFVLGLTPYLYLPLR
                     AAMDPPMDANNPTTLGRFWYVVSGGNLTGSFFSFGPAELPGRMLFYAGHLLQNMPFLV
                     VMVALTGAVVVFLRDRAAGTFLGFLFFGWLFYSIENDIPDIELYFIPTYLVLSLWAAA
                     GFGALLDEAESLTGGLSRAARWGAIGALCVVVLLLPLFGVRETYAGADMSGVYRGQKE
                     ISAVAENAAPNATVLHHRSSMWYMVLVEKRRRDLTIVDPFYHNTDASWADLVWPADID
                     LATTDRRYGTDDLSGAKSARMAAEKGRVYLLDQESTNLQPFRQAGFRVAVVEEGYLYE
                     LLPPGREPYAN"
BASE COUNT         1098 a         2075 c         1998 g         1108 t
ORIGIN      
        1 aagagcctcg taccgtacag agaccgcagc gcccaccgcc ttgtctcttc ggccgaggat
       61 cccttcagcg acagccagat caggcccagc cagaccacgc acagcacgtc gtagagggcc
      121 aggtagaaca tggtccagcc ggccccctga ctccaggtcc agcgggaaga aaaagatcac
      181 acccaaagca gcgagcacgg cgatccacgc gcgaacggcg tcacctttgg gggtcatccg
      241 acgtacggag gagatcagac caccgacccg cctgaacata acccgggctt gatcccgtcg
      301 agacttgcgc cccccatcgg ccatgcaggt actctagatc acccgtcaag ccccgcacac
      361 gggtggtgga ggctagcggg gggtgccgtc cgcccgaccc cgcgaagcca ggtacagcag
      421 tgtttgcatc acggtagcgg cgagggtgcc gagggcgagg gaataggaga ccctctcgac
      481 cacgccgacc ccgagcgtaa ccaggcagac ggcggaggct agcagcccga cgaaccaccc
      541 gagcgcggcg atccttccac gccccagggc gaagaggccc tggctgaccg tctcggagag
      601 caggtagaag gcggccatcg ccgccagcgc cacgagcacg gcccgttcca ccacaaactc
      661 ctccccggcg aagagcctga gcccccagcc cccgagcagc cacgtgcccc cgaccatgaa
      721 gactcccacg aggccaacga ccccgagcgc cctcagcagg aaacgctcga aggcggcccg
      781 accgtcggtc gagagaacgc ggctcgcgcg gggcaagagc gccccgatgg ctgggctcag
      841 cacgtactgc gggatacggg taaagatgag ggccgccgcg aacaacgata cctgggccct
      901 ggtgccgccg atcagatctg ccaggatggg cccgccgttc atcagcgcct gggcgaaggc
      961 catacacgcc agcaccggcg cggcgaactg gagggcgtgc cccgtcgaga acggctgtcc
     1021 ctcccccttc ggcggtggca cgcgcgccgg cctgacggcc agaacaccga tgagcggcgc
     1081 gagcacgatg gcgatggccg gcccgaacac gccccacccc acgacgagca ggaccgcggc
     1141 tatcagcacc cgccccgccg actcggccac gatctggacc ccgagcctga gcggctgcct
     1201 gtgcccgttg aagacgccgc gccggaaata ctccggcgcg tagagcagga cggcggccac
     1261 gaacgccacc gtgagccaca cgctgccgaa gaggttcgtg agcgccgggc tggccagcaa
     1321 cgcaccgagc aagaacaccc ctgacagccc gatctgccaa cgcctcaccg agacgaccac
     1381 gggctcccaa tcctccccct tcgcctcccg ctcggccaca taccgcccca gggtctgggt
     1441 cgccccgacc cagagcacct gcaccgtcag gaaagtggcc gaccacaaca gcgcgagcgg
     1501 cgcgtagttc tgcacgccga gaaagcgtgt agagagaccc tggaacgcaa acgtaagcac
     1561 ccacgagacg ccgaaggaga cggcgatgta aacgcttcca gctccagcac tctgggttac
     1621 tcttgacctg gagaggaact tcatggttgt ttagctatct gctttcagct atctgctctt
     1681 gtgtcctagt tagaacctgg cccgaatatg ctaccgcggt tcgaaccggt aggaaaccgc
     1741 gcaccagcct ctcacacgct cttgctgata gctaatagct tgaatttacc taagccacgt
     1801 cgagatgatc accctgaaga cgcccagcgg gtaggcgagc ttcgggggct tcttggtctc
     1861 gccggcggcg cggctcatca tggagaccgg gacctgctcg aagcggagct tgctcttgag
     1921 cccttcgagg attatctcgt tggcattgaa gcggtcctcg cgcaggttga gcttgtggag
     1981 ctcggacacc ctgatggcgc ggtagccgtt cgtgcagtcg gagaccttga cgccggtgag
     2041 aagcgagatc aggcgcgaga agaatacgac cccgatgtga cggacgctcc ccgcctcctg
     2101 gtagtaaccc atgaaccttg agccgagcac gaagtcggcc ctgtcctcga ttatgggccc
     2161 aacgagacgg ccgagctcct ccggcttgta ctggccgtcg gcgtcgaggt tgatgacgac
     2221 cagggccttc tcggcccgcg ctatggcgaa gccggtccta agggcgtcgc cctgcccgcg
     2281 attgaccacg tggctcacca cgggcacgcc ctcggccctg gcgaaatccg ccgtcctgtc
     2341 agccgagccg tcgtccacga cgaccgtctt gacctggtgg cccaggacct catcggggat
     2401 gcggcgcagc acgtccctta tgccgccctc ttcgtcgtag gccggcacga ttatcaggac
     2461 ctcgccggga tgtccctcga ccctctcttc ggaggagagg tatctctcgg agtactcctt
     2521 tacggcgagg ccggtgacga tctcgccgtt gcgccgggtg gcctcccgca cgcgggccag
     2581 gaggttcagg aagagcccga agaggagcag cacggcgatg gagagcagcg cgaaaccgcg
     2641 gtcctcgagg ccgagcaggc gggtgaagat gccgaaaaac tggggtacca ccgagacgag
     2701 ggccacgccg atggcgatga gcagcgagag gagcaggtcg agcctgctcc ctcccccgcg
     2761 gtacctgtag gcgccgtacg ccgcgaagag cagggctatg acgacgccgg cgatgcttac
     2821 gtgttcgacc acctgctacc ggtgcccgtc ctgctggggc acgacctctt cccgcggcct
     2881 ccacaacgag gtggctggga tcttcgaggt gtcgcagcgg tcggcgtact tgcgggctat
     2941 ctcggttacc tcctccagca gaccttcgct cagggtggtc ggctgcaggc ccagatccag
     3001 gaaacggtcg ttgcggacgt gcaggtcgtt ctcgtcggcc tcgttgcgcg ggttctgcac
     3061 gtagtcgatc tcggcgcccg tgcgctcgga gacaagcgtg gccaggtcgc gcaaacggtg
     3121 ggtctcggtc atctggttgt agacgttgac ccggtcgccc ttctcgggcg ggttctccag
     3181 ggcgagctgt atgcagcgca cggtgtcctg gatgtggata aaggcgcggg tctggccacc
     3241 ggtgccgtgg acggtgagcg ggtagcccac ggcagcctgc atcaggaagc ggttgagcac
     3301 ggtgccgtag tcgccgtcgt agtcgaagcg gtttatgagg cgctcgtcta gcctggtctc
     3361 ggcggtctgg gttccccaca cgatgccctg atgcaggtcg gtgacccgcg tcaggtcgtt
     3421 cttgttgtag taggcgaaca ggagctggtc ctgggtcttg gtcatgtggt agatgctgcc
     3481 cgggttgggc gggtagagga tctcctgctc taccatctca ccctcttcgg tctcgacctt
     3541 gaccgtcagg tacccctcgg ggatctgcat cccggccgtg ccgtagccgt agacgcccat
     3601 ggtcccgagg tggaccacgt gggtgtcgag cccgctctcg gtgatcgccg caagcaggtt
     3661 gttggtcgcg ttgaggttgt tgttgacggt gtagcgcttg tggtagctgg acttcatgga
     3721 gtagggcgcg gcccgctgct cggcgaagtg gatgaccgcg tcgggccgcc actcgaggag
     3781 caggtccaga agcttctggt agttctcggc gacgttgaag ttgtggaagt cgatcctgtt
     3841 gccggtcaga tcctcccaga cacggaggcg ctcacccatg ggccggatcg gggtcagaga
     3901 ctcacactcc aactcgacgt ctatcttgcg ccgggagagg ttgtcgacta tggccacctc
     3961 gtatccctgc gccgagaggt gcagcgaggt gggccagccg caaaagccgt cgccgcccag
     4021 aacgagtacc ttcatctcac gcgctccctt cttgctctac gctaatcgta ttctaccgtt
     4081 tgggatcgag ccggaaccag gctcgacagc cagcgattgt aacggaaaag ttccggggat
     4141 tactaatcgt gggggtgtcg tcctttgtgg tctctcccgc aagccggccc cgcagcccca
     4201 ccctcgagca cggcccgggc gacccggcgg acgctatatt agcgcagatt aatgtaaatt
     4261 aacatcgaaa cgtcgtccgg ttacaatagc gcccgcacgt aagtctggcg aagggcatcc
     4321 cggaaagtgg cactggaaca gcaaaaaccc gcccacccac ccgcgaaacc atccccggta
     4381 tccgggggac tggcacgcgt ggcggccgtc tctctggccg ggatcggcgt cttcctgttc
     4441 gtcttcgcgc tctacgtgat gacgctctcc cccacggtgc tgtacttgca ggacccgaag
     4501 cttctcgacg cggtgatggt ccagatgcag gtcgccgtgc tcggcatgac gcaccccacg
     4561 ggctacccga cctatctgat gctgacccac ctcttcacct acctgccggt gggggacccc
     4621 gcctaccgcg tgaacctcgg ctccgccttc tacgcggccc tggccgtggt ggcggtctac
     4681 ctgacggggc ttctgctaac gaggcgggtc gtggccgcgg tcgctggcgc cctagccttc
     4741 gggcttggcg cggcgatctg gtctcaggcc gtcatcgcgg aggtctacac cctgaacgcc
     4801 ctgctcgtgg cggcaaccgt cggcgtcctg ctgctctggc gcctgcgacg gatggaccgc
     4861 tacctcttgc tctcggcctt cctgatggga ctctgcctga ccaaccacct taccagcggg
     4921 ctgctcctgc cggcgggcgt ccttctcgtt gccctcgtgg accgggccaa gctcaaggac
     4981 gtcaggctcc ttctcaaggg tgccgggctc ttcgtgctcg gtctcacgcc ttacctgtac
     5041 ctcccgctga gggcggccat ggacccgcca atggacgcga acaacccaac gaccctcgga
     5101 cggttctggt acgtggtcag cgggggcaac ctgacgggct cgttcttctc cttcgggccc
     5161 gctgagttgc ctggcagaat gctcttctac gcggggcacc tgctccagaa catgccgttc
     5221 ctcgtggtga tggtcgcgct taccggcgcc gtggtggtgt tcctcaggga ccgcgcggcg
     5281 ggtacgttcc tgggcttcct gttcttcggc tggctcttct actccatcga gaacgacatc
     5341 cccgacatcg agctgtactt tatccccacc tacctcgtgc tctcgttgtg ggccgccgcc
     5401 gggttcgggg cgctgctgga cgaggccgaa tccctcaccg gcggcctctc gcgggcggcg
     5461 cggtgggggg ccatcggagc gctctgcgtg gtggtgctcc tcctgccgct gttcggcgtg
     5521 cgcgagacct acgctggggc cgacatgagc ggggtctacc ggggacagaa agagatctcg
     5581 gcagtggccg agaacgcggc tccgaacgcc accgtcctgc atcacaggag cagcatgtgg
     5641 tacatggtgc tcgtggagaa gcgccgcagg gacctcacca tcgtagaccc cttctaccac
     5701 aacacagacg cttcctgggc cgacctcgtg tggcccgcgg acatagacct tgcgaccacc
     5761 gaccgccgat acggtaccga cgacctctcc ggggccaaat ctgccaggat ggccgccgag
     5821 aagggccgcg tctacctcct ggatcaggag tccacgaacc ttcaaccctt caggcaggcc
     5881 ggcttcaggg tggcggtcgt agaagaaggg tacctctacg agctgctacc gcctggtaga
     5941 gaaccctacg ccaattagag cggtcagtga ttggagcggt cagctttcag cttttggctg
     6001 cgagagatgc aaacctgagg aaggtaacag gcgatgggta tcaggtacta ttctccagac
     6061 ctgatacccg tagccttctc ccgccgggtg gtcgtttttc gaggatcgct ctagtctttg
     6121 gctgattgcg gaccgctgat agccgaaggc tgaaattcgc caccttcggc gatgcggcat
     6181 tcttcggagg cacacttccc aagcgtgccg agttgcgatt ccaggcgctt cttgaggtcg
     6241 gacggggccg attcgtacac gttgcgcagc tggtagggg
//
LOCUS       DBNGVD010000253         3428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_421148, whole genome shotgun sequence.
ACCESSION   DBNGVD010000253 DBNGVD010000000
VERSION     DBNGVD010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_421148"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC16CM_06945"
     CDS             complement(<1..258)
                     /locus_tag="AC16CM_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3139601.1"
                     /translation="MQEMVIIGLIVLVIFGPKNLPSMARDVGRFVSDARRSVEEFKQD
                     LVSEKEDGQELRNGRDPEASREPEENAGRKGVAAIEGSAQRT"
     gene            complement(279..545)
                     /locus_tag="AC16CM_06950"
     CDS             complement(279..545)
                     /locus_tag="AC16CM_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002863454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3139602.1"
                     /translation="MAGLASPSHLIILLALVLLLFGAKRIPELAKGVGTGIKELHEGI
                     STGQGAGAEAEALESEEAGSGAGSATRIGSMGEPVAARADRGGL"
     gene            complement(673..1440)
                     /locus_tag="AC16CM_06955"
     CDS             complement(673..1440)
                     /locus_tag="AC16CM_06955"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3139603.1"
                     /translation="MNGMDRQRFEDLKDGYVLGALPEEERLEFEQYLAAHPDLQSEIE
                     ELGTLAGLLALSVSEQEPPPDLRRRIMDTVEAEAVRPAEMTPPHAGRQFWIAGLWDAV
                     GLRELALAAAAVLVVGLFAWGMLLQGEVRELQGRVQSLQSQPQVRSQDPQMIALGGTG
                     AEQEARAKLITLKGDRAVLVAENMPPAPEGKTYQIWVIKGDTPKPSGLFEPRGDSVAA
                     VVENSVERADAVAVTIEPEGGSKEPTTDPMLVGKVKT"
     gene            complement(1437..2024)
                     /locus_tag="AC16CM_06960"
     CDS             complement(1437..2024)
                     /locus_tag="AC16CM_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3139604.1"
                     /translation="MGDGIRQYLLYADEDLMPLLEVGDAEAFAVLYDRHGRMAYSLAY
                     RMMGEKQEAEDVVREAFIKVWRSAGGYRVGRGSVRTWILSIIRNRGIDQIRSHGRRGR
                     MQDKVEASAPRSEPSEAFAETWKNAQQEQVREALNTIPREQLKILELAYFSGYTHAEI
                     AERLELPLGTVKGRMRLGLQNLRDYYQARGAAVPR"
     gene            complement(2212..2970)
                     /locus_tag="AC16CM_06965"
     CDS             complement(2212..2970)
                     /locus_tag="AC16CM_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3139605.1"
                     /translation="MTDAPNSEGFGMRSSTSRRRFLTGSAAALAGGALMAVPGMARAH
                     STPEPPTDIDVLNYALTLEHLEYAFYRDGLRKFDEQDFRSSNLFSGSGNLLRPTVYEN
                     FKRIRNHEGAHVDTLKSVIRSLGGRPVPEAEYNFRRTAFTSVEKFVSVAQFLENTGVS
                     AYDGAIANIEAAALLTAGATIATVEARHASYLNLLTKDVPFPRAFDEPVAPRAICEAI
                     QAENGGFIVSAPKPYGPYRSLDALCKRLPNTITP"
BASE COUNT          554 a         1114 c         1060 g          700 t
ORIGIN      
        1 ggtgcgctgc gcgctgcctt cgatcgctgc aacccctttg cgccccgcgt tctcttctgg
       61 ttcccgactc gcttccggat cccggccatt acgaagctcc tggccgtcct ccttttcgga
      121 gacgagatcc tgtttgaatt cctctacaga gcggcgggcg tcgctgacga accggccgac
      181 gtctcgggcc atgctaggga ggttcttggg accgaagatg accaagacaa tgagacctat
      241 gattaccatc tcctgcatgc cgatgccgaa cacggggctt agagacctcc ccggtcggca
      301 cgcgcggcaa ccggttcgcc catcgacccg atgcgggttg cgctaccggc tccactacca
      361 gcttcctcgc tctcgagcgc ctcggcttca gctccggccc cttggccggt ggagatccct
      421 tcgtgcaact ccttgatgcc cgttcccacg cccttggcca actccggtat ccgcttggcc
      481 ccgaacagca gcaagacgag cgcgagcaag atgatcaggt gggaaggcga tgcgagtcct
      541 gccaacatgg gtgtcgatcc tttgcttcgt ttgtgtgtca ccggttattg ggcatacgat
      601 cttgggggag gggttggatc agcgccttct gggcaggtgg agaccttgtt ctgccggttc
      661 ccttactctc ctctaggttt tgaccttgcc gaccagcatc ggatcggtcg tcggctcctt
      721 cgagccgccc tcgggctcga tggtcacagc cacggcgtcg gcccgttcca cggagttctc
      781 caccacggcc gcgaccgagt cccccctggg ctcgaagagg ccgctcggct tcggggtgtc
      841 gccctttatg acccagatct ggtaggtctt gccctctggg gctgggggca tgttctcggc
      901 aaccagtacc gcccgatcgc ctttgagggt tatcagtttc gcccttgctt cctgctccgc
      961 acccgtgccc ccgagcgcga tcatctgggg atcctgggac cggacctggg gctggctttg
     1021 gaggctctgt acgcggccct gcagttcgcg cacctctccc tgcagaagca tgccccaggc
     1081 gaacaggccg accacgagca cggccgcggc ggcaagcgcg agctccctga gccccacggc
     1141 gtcccacagc ccggcgatcc aaaactggcg gccggcatgc gggggtgtca tctccgccgg
     1201 gcgtacggcc tccgcttcca ccgtatccat aatcctgcgg cggagatccg ggggcggttc
     1261 ttgttccgaa acggagaggg ccagaagccc tgcaagggtg cccagctcct ctatctccga
     1321 ttgcaggtcc gggtgcgccg cgaggtactg ctcgaactcc aggcgctcct cctccggcaa
     1381 ggcgcctaga acgtagccgt ccttgaggtc ctcgaaacgt tgccggtcca taccgttcat
     1441 ctgggcaccg ccgctcctcg cgcttggtaa tagtctcgga ggttctgcag ccctagcctc
     1501 atccggccct tgaccgtccc caggggcagc tcgagacgct cggctatctc ggcgtgggtg
     1561 tagcccgaga agtacgccag ctcgaggatc ttaagctgct cgcgcggtat ggtgttcagt
     1621 gcttctcgga cctgctcttg ctgggcgttt ttccaggtct cggcgaaggc ctcactgggc
     1681 tcggatctgg gcgcggaagc ctcgaccttg tcctgcatcc tgccgcgacg cccgtgggag
     1741 cggatctggt cgatgccgcg attgcggatt atggagagga tccaggtcct gacgctgccg
     1801 cgccccaccc ggtagccacc ggcggagcgc caaaccttga tgaacgcctc ccggaccacg
     1861 tcttcggcct cctgcttctc gcccatcatc ctgtacgcca gcgagtaagc catgcgcccg
     1921 tggcggtcgt agaggacggc gaaggcctcg gcgtcgccta cctctaagag cggcatcagg
     1981 tcctcgtccg cgtaaagcag gtactgtcgg attccatccc ccacacccct attctacgaa
     2041 actctcgcag gcggggcgca cggccccgcc tgctaggggc tgatctcgag cgttcgtacg
     2101 tcgccattga tctgaatccg ccttccacgt gtgcgagcgg ttgggttcgt cgaacacgaa
     2161 gcggggccgg aggtcgaccc ccggccccgg tcttctgtgt ggggttcttc cctacggcgt
     2221 gatggtgttg ggaagcctct tgcacagggc gtcgaggctc ctgtagggac cgtagggctt
     2281 cggagccgag acgatgaacc cgccgttttc cgcctgtatg gcttcgcaga tcgcccgcgg
     2341 tgccaccggc tcgtcgaaag ccctggggaa gggcacgtcc ttggtgagga ggttgaggta
     2401 agaagcgtgg cgagcctcca ccgtggctat cgtcgccccg gcagtcaaaa gtgccgccgc
     2461 ctcaatgttg gcgatcgccc cgtcgtaagc ggataccccc gtgttctcca ggaactgcgc
     2521 cacggagacg aacttctcca cgctggtgaa ggccgtcctc ctgaagttgt actcggcctc
     2581 tggcacgggc cttcccccaa ggctcctgat cacgctcttg agcgtgtcca cgtgggcgcc
     2641 ttcgtggttg cggatgcgct taaagttctc gtagacggtg ggcctaagga ggttgcccga
     2701 tccggaaaag aggttgctcg agcggaagtc ctgctcgtcg aatttcctaa gcccatcgcg
     2761 gtagaaggcg tactccaggt gctcgagggt gagggcgtag ttgaggacgt cgatgtccgt
     2821 ggggggctcg ggggtgctgt gcgccctagc catccccggt accgccatca gcgcgccgcc
     2881 cgcgagcgct gcggccgagc cggtcaagaa gcgtcgccgg gaggtcgacg agcgcatccc
     2941 gaaaccttct gagtttggag catcggtcat gtatccactc tccttatttc tgtgcggggc
     3001 cgaagtcccg gcttattgac ggggttcgtt ccccgataaa aagcggatac gaaagcgggg
     3061 aactcctcag atcaccacgc gccaacaagt gtgctagcgg aggcgcgcta gacgaacttt
     3121 ttagaatgcc gttagtgcga agttcggttc gacggcagcc cagtggacaa gaaattctcc
     3181 ggtagttgcg ttgttacgag tcgcaaccgc ttgatacaca atgccgcaac gaaagctacc
     3241 aggtcgccgc cattggatgc cgaaggggtc actgggtgcc gctctcgagc acggaccgcc
     3301 gcgctcgacg ggtcgcttgc tacgtcgaac ttccgagaat tgaactgcct gcgaacttcg
     3361 gagtacacct cttctaccac tcgggtgaat aggagtagtc ttcgctgcta ccatgccaac
     3421 cagaaggc
//
LOCUS       DBNGVD010000254         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4611025, whole genome shotgun sequence.
ACCESSION   DBNGVD010000254 DBNGVD010000000
VERSION     DBNGVD010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4611025"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="AC16CM_06970"
     CDS             <1..888
                     /locus_tag="AC16CM_06970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3139606.1"
                     /translation="PDMLIGLQVPRFTWPQGTSGIGPKLAEVGRTADEAGFASLWVMD
                     HFFQIEAVGEVDEPMLEGYSALTYLASVTERVKLGTLVTGVHYRYPGLLIKTATTLDV
                     LSGGRAYLGIGAGWNERESRGLGVPFPSTRERFERLEETLQIAQQMWSGEVGTYEGEH
                     YHLAETLNEPQALSEPHPPIMIGGMGEQRTLRLVAQYADACNLFTYGGPDVIRHKLDV
                     LRGHCEDVGRDYGEIERTALGTANLSEDGMSAEDLIGFCREMNEAGIQHLIFNMPNVH
                     EIEPLETFAEKIIPAVAEF"
     gene            complement(990..1466)
                     /locus_tag="AC16CM_06975"
     CDS             complement(990..1466)
                     /locus_tag="AC16CM_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139607.1"
                     /translation="MADDGDTNEVQGYGQEVAREPSPALRRLDRLVGTWELSGDVGGT
                     VTYEWMEGGFFLIQWINMVDKEDGTRSTGMEVIGHERPLGQEPSADVKSRFYSDTGGT
                     LDYTYELEDDTLTIWFGDKTSPAYYRGILSEDGDRLIGAWHYPGGGGYEANSTRVR"
     gene            complement(1518..1973)
                     /locus_tag="AC16CM_06980"
     CDS             complement(1518..1973)
                     /locus_tag="AC16CM_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3139608.1"
                     /translation="MTLPADDRILITREFDAPKHLVYRAWTEPGLIERWWGGERGEVT
                     SVEVDLRVGGVWRCVMVLEDGTEAAFRGEYREVVANERIVSTEVYEGAPGVQALNTVT
                     FEESEGRTTLKVLVEHASKEARDAHLASGMETGMQEGMNLLEGLVFELG"
     gene            complement(1975..2343)
                     /locus_tag="AC16CM_06985"
     CDS             complement(1975..2343)
                     /locus_tag="AC16CM_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3139609.1"
                     /translation="MARAATTTDAFNAVAEPRRREILDLLVDGERPVGDMVGQLGMSQ
                     PLVSKHLRVLREVGAVEVREEGRRRLYRLNAEALRPIHDWVSGYERFWSGRFGRLDAV
                     LEDLKRREGSGGDGGGSKEG"
     gene            complement(2464..>3665)
                     /locus_tag="AC16CM_06990"
     CDS             complement(2464..>3665)
                     /locus_tag="AC16CM_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139610.1"
                     /translation="QALGAAALIPGSLAILGAAFEGERRGAAVGTWAAFAGIAAVLGQ
                     VLGGFLVDAISWRVAFMVNVPMAIVVLLIVFRYVPESRAEHPRAFDIPGVVLITVGLG
                     GVVYGLIGASDQSLGPWEVAAMVVGTVALAGFVIVERHSPEPLVPLHLFGSRNFSGAN
                     LMTFLLYAAFGGALFLLPIVLIEVHGYSATAATGALVPFAVITAVMGRWAGGLVTRFG
                     EKLPLVVGPTLAATGFILFAVPGLGGSYWTTYFPAVVVLGVGMSLVFGPLSVAILNAV
                     ETEHSGLGSGVNRSVQRTAKLLGLAVLAFLLLAAFDSSLDARVDALDLPPGQKAALRA
                     EKVHLGANDVLKGASNEDRIAIDHAVDQAFISAFRIAMIVSAGMALTSAAAAAFLIQG
                     KRDGEPG"
BASE COUNT          681 a         1261 c         1094 g          629 t
ORIGIN      
        1 ccagacatgc ttataggttt gcaggtaccg aggtttacct ggccccaggg cacctccggc
       61 ataggcccga agctggctga ggtcggcaga accgctgacg aggcagggtt cgcgagcctg
      121 tgggtcatgg atcacttttt ccagatagag gcagtcggcg aggtagatga gccgatgctc
      181 gagggctaca gcgcactcac ctaccttgca agcgtcaccg aacgggtgaa gctcggaacg
      241 ctggtgaccg gcgtccacta ccgctacccc gggctcctga tcaagaccgc gaccacgctc
      301 gacgtgctct cggggggaag ggcctacctc ggcatcggcg cgggctggaa cgagcgcgag
      361 tccaggggcc tgggcgtgcc ctttccatct accagggagc gcttcgagcg gctggaggag
      421 accctgcaga tcgcgcagca gatgtggtcg ggggaggtcg ggacctacga gggagagcac
      481 taccacctcg ccgaaaccct caacgagccc caggccctca gcgagccgca cccgcctatc
      541 atgatcggtg gcatgggaga gcagagaacg ctcaggctcg tggcgcagta cgctgatgcc
      601 tgcaacctgt tcacctacgg tggccctgac gtgatccgcc acaagctcga cgtcctgcgc
      661 gggcactgcg aagacgtggg aagggactac ggggagatcg aacgcacggc cctcggtacc
      721 gccaacctct ccgaagacgg catgagcgct gaggacctca taggcttctg ccgggagatg
      781 aacgaggccg gcatacaaca cctgatcttc aacatgccca acgtccacga aatagaaccg
      841 cttgagacgt tcgccgagaa gatcataccg gccgtagccg aattctagag cgacgcttgt
      901 cgaaagtgag ggaaggcttc aggcaacagg catcgggtga ttttcctgtg gcccgagacg
      961 tcctcccgaa gaatcgctcc ggggcgaggc tagcgtaccc tggtagagtt ggcttcgtac
     1021 ccgcctccgc ccggatagtg ccaggcgcca ataagcctgt caccgtcctc gctcagaata
     1081 cccctgtagt aggctggcga ggtcttgtct ccgaaccaga tcgtgagggt atcgtcctcc
     1141 agctcgtagg tgtagtcgag ggtaccgccg gtgtcggagt agaagcggct cttcacgtcc
     1201 gcgctgggtt cctgccctag cgggcgctcg tggccgatga cctccatgcc ggtgctcctg
     1261 gttccatctt ctttatccac catattgatc cactgaataa gaaagaagcc gccctccatc
     1321 cactcgtagg taaccgtccc ccccacgtca cccgagagct cccacgtgcc gaccagcctg
     1381 tcgagcctcc tcagggctgg gctgggctca cgtgccactt cttgcccgta tccctggacc
     1441 tcgttcgtgt caccgtcgtc cgccatggtt ctccttcctt gcggctgccc ttctcctgtc
     1501 ccgcgtcttg ctcgtcatca gccgagctcg aacaccaacc cctccagcag gttcatgccc
     1561 tcctgcatcc ccgtctccat gccggaggcc aggtgcgcgt cgcgggcctc cttgctcgcg
     1621 tgttcgacga ggacctttag cgtcgtgcgt ccttcgctct cctcgaaagt tacggtgttg
     1681 agcgcctgca ccccgggagc gccctcgtag acctcagtcg agacgatccg ctcgttggca
     1741 acgacctccc ggtactcgcc tcggaaggcg gcctccgtcc cgtcctcgag gaccatcacg
     1801 cacctccaca cgccccccac ccgcaggtcg acctccacgc tcgtcacctc cccacgctcc
     1861 ccgccccacc agcgctcgat gagcccgggc tcggtccacg ccctgtagac caggtgcttc
     1921 ggcgcatcga attctctcgt gatgaggatc cggtcgtccg ccggaagcgt caccttagcc
     1981 ttctttcgac ccaccaccgt ctccccctga gccttcccgc cgcttgaggt cctccagtac
     2041 agcgtcaagc cgcccgaacc ggccggacca gaaacgctcg tagccgctca cccaatcgtg
     2101 aatcggtctg agcgcctcgg cgttgagccg atagagccgc ctccttccct cctcacgcac
     2161 ctccacagcc cccacctccc tcagcaccct gaggtgcttg gatacgaggg gctggctcat
     2221 cccaagctgg ccgaccatgt ctcctacggg ccgctcccca tcgacgagga ggtccagaat
     2281 ttcccgcctg cggggctccg ccaccgcatt gaaagcgtcc gtcgtggtag ccgcccgtgc
     2341 catgagaaga ttatacataa ctatatagtt atgtatcaag acccggacag cagcgtggga
     2401 ctgaaagctc gttccattag cacaggaacg gtggccgctc tggcctggtg tgtggggatc
     2461 gggctagccg ggttccccgt cccgcttgcc ctgaatcagg aatgcagcgg cggctgcgct
     2521 cgtgagggcc attccagccg agacgatcat cgcgatacgg aacgcgctta taaaggcctg
     2581 gtccacggcg tggtctatgg ctatcctgtc ctcgttgctc gcccccttca gcacgtcgtt
     2641 cgcgccaaga tggaccttct ccgcgcgcag ggccgctttc tggccgggcg ggagatccag
     2701 ggcgtccacc ctggcgtcca ggctgctatc gaaggctgcc agcaggagga acgcgaggac
     2761 cgccaggccg agcaatttcg ccgttctctg gaccgacctg ttgacgcccg agccgaggcc
     2821 ggagtgttcg gtctcgaccg cgttcaggat cgcgaccgaa agcggtccga acaccaggct
     2881 catcccgacg ccaagcacca ccacggccgg aaagtaggta gtccagtatg atcctccgag
     2941 ccccggaaca gcgaagagga tgaaccccgt ggcggccagt gtggggccga ccaccagtgg
     3001 tagcttctcg ccgaagcggg tcaccagccc gccggcccag cgtcccatca cggccgtgat
     3061 tacggcgaac ggtacgagcg cgccggtcgc ggcggtggcg gagtagccgt ggacctcgat
     3121 caggacgatc ggcagcaaga acagcgctcc cccgaacgcg gcgtaaagca ggaacgtcat
     3181 caggttagcg ccgctgaagt tgcgcgagcc gaagaggtgg agtggaacca gaggctcggg
     3241 cgagtgccgc tcgactatta cgaacccggc cagcgccacc gttccaacga ccatggctgc
     3301 cacctcccac ggaccaaggc tctggtctga ggccccgatc aggccgtaga cgacaccccc
     3361 gaggcccacg gtgatcagca ccacccccgg gatatcaaag gcgcgcgggt gttcggcgcg
     3421 gctctcggga acgtatcgaa acactatcag aagcacgact atggccatcg gcacgttgac
     3481 catgaaggcg acccgccacg agatagcgtc gaccagaaag ccgcccaaga cctgtccgag
     3541 cacggcggcg atgcctgcga acgccgccca ggtaccgacc gcagcgccgc gcctctctcc
     3601 ttcgaatgcg gcaccaagga tggccaacga gccggggatc agggctgcgg caccgagggc
     3661 ctgaa
//
LOCUS       DBNGVD010000255         5311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_239182, whole genome shotgun sequence.
ACCESSION   DBNGVD010000255 DBNGVD010000000
VERSION     DBNGVD010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_239182"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC16CM_06995"
     CDS             <1..604
                     /locus_tag="AC16CM_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139611.1"
                     /translation="LATIIESLDLEEAVLIGHSTGGGEVARYVGRHGTSRVSKVVLVG
                     AVPPLMLKTEANPDGLPIEVFEDIRSGVAADRSQFFKDLSEPFFGINRPGSNVSQGVR
                     DAFWLQGMRGGLKNELDCIKAFSETDFTEDLRKFDVATLIIHGDDDQIVPIVASALAS
                     SKLVKNSTLKIYEGGDHGLTDTHKDQLNRDLLAFLLRGTH"
     gene            789..1637
                     /locus_tag="AC16CM_07000"
     CDS             789..1637
                     /locus_tag="AC16CM_07000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3139612.1"
                     /translation="MTSENNHIEAATDAAAPDTVVFIHGLWMTPRSWEKWTERYEARG
                     YKTIAPAWPGMEGEVEALNADPSPIAELDIDQIVDHYTRIIGELDKPPIIMGHSLGGT
                     VLQVLLDRGLGAAGVGIAPGTVKGVPDLPLSTLRATLPALRNPFKRHSAVPLSAKEFH
                     YAFANTLSREESDEIYDRYHVPCAVNVLREAAFASLHRDAPTKVDFANDDRAPLLFLT
                     FEHDHIIPPEVGRHNAEKYSGSQAVTEFQEYLGRPHFPGAPGYEEVADHAIEWAAEHA
                     VARASV"
     gene            1754..2491
                     /locus_tag="AC16CM_07005"
     CDS             1754..2491
                     /locus_tag="AC16CM_07005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3139613.1"
                     /translation="MLYRNVADGVHRIGEHFVNWYLVEENGHVCVVDAGLPSSWSSLL
                     EALHAIGRKPHDIEGLVLTHAHFDHIGFAERARVEFGVPVWVHENDAPLTKRPWLYMT
                     ERSPVSYLGRKNLPIVSSLIRAGALRVGPIREVRRYGNEGTLDVPGSPKVLFTPGHTL
                     GHCALHFPERDVVVAGDALVTHDPYTDTRGPRIVARGATADSERALSSLELLADTDAR
                     MLLPGHGEPWTDGAERAVREALRVGAL"
     gene            complement(2634..3605)
                     /locus_tag="AC16CM_07010"
     CDS             complement(2634..3605)
                     /locus_tag="AC16CM_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3139614.1"
                     /translation="MKVATHIVFAECCWFATSAVLDVHYGTSAVLVVAATSILPDADD
                     PKSWLGHRLGSVPQELRRLLAPGSCFHSLLALLFCTTALGLPLWWVADQPALAVAAFV
                     GYGSHLFADMMSLGGAQLFWPSQAIAVLPGRDQYRVVSGSASERVFVAVALLLALLFY
                     PVSRAGFDGLIYRMGGSDRVYGKVQKVTDGDTVSVEVYGQLQHVRLIGVDTPETVAPD
                     QPIGCYGKKASAYTKKTLSKRLVRMEIPRVGDSEDAYGRTLAYLYLDADHNGSYEHPF
                     NEDLLKLGYARTTTFSHAYRREFERLRERAEEQETGLWDDCTATTPY"
     gene            complement(3613..4185)
                     /locus_tag="AC16CM_07015"
     CDS             complement(3613..4185)
                     /locus_tag="AC16CM_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139615.1"
                     /translation="MDFDTRQADRGRRGPGSSRERETGRLPRPDDDRDTLTIPAGARK
                     AALVHLALAAWRAGEIDPRKLGQVRGREVDEAGFQRALYRSSMGEIEHVLRPVLKGRR
                     VEVQGRLAGAGRTALERLGAEVEELGLEDEKRYIDMTPAIFVLAALAMAVRYVSLGLD
                     DQDLYILAGIVFAVMYGLRPFFSRMQRPRD"
     gene            complement(4198..5109)
                     /locus_tag="AC16CM_07020"
     CDS             complement(4198..5109)
                     /locus_tag="AC16CM_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3139616.1"
                     /translation="MTRGPIISLRDVSKSYGAFELGPIDLDVEPGCVVAVVGPVGSGK
                     STLLKMLMNLVPPDSGEVSLFGGAFPEDEVAIKQKIGFAPERATGYEDLSPRTLRELV
                     SYFYPGWDQKAYEVPLSGTEIEEDRPFEALSSGTQRLLIVALALAAGSELLVLDEPTA
                     GLDPFARSRVLAGISHFIADERHRNRTVVFATHAMEDVRLIADHIAFLVDGELLGLFE
                     TGALQERWTAFWPERAPEGDIPGMVELDSDDPVRIVSDSPGETAEALRARNIGVLRTG
                     ALELEEILSHLMRQRAEGRAGSPRARE"
     gene            complement(5106..>5311)
                     /locus_tag="AC16CM_07025"
     CDS             complement(5106..>5311)
                     /locus_tag="AC16CM_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139617.1"
                     /translation="LTRQGRGTVVAEITEEKLRARRHEAVKAAFAEAVGTGRRAGLTG
                     DELHAVLDETLQGDQTETGGDRL"
BASE COUNT          971 a         1734 c         1651 g          955 t
ORIGIN      
        1 cctcgcaact atcatcgaaa gcctcgacct ggaagaagcg gttctgatcg ggcactccac
       61 cgggggtggg gaggtcgccc gctacgttgg acgccacgga acgtcacggg tgtcgaaggt
      121 tgtgttggtc ggggccgtgc cgccgctgat gctgaagacc gaagcgaacc ccgatgggct
      181 ccccatcgag gtattcgaag acatccggtc cggggttgcg gcagatcgct cacagttctt
      241 caaggacctt tccgagcctt tcttcgggat caacaggcct ggttccaacg tttcccaggg
      301 tgtgcgggac gctttctggc tgcaaggaat gagaggaggc ctcaagaacg agctcgattg
      361 catcaaggct ttctcggaga cggatttcac ggaagatctc agaaagttcg acgttgctac
      421 cctgatcatc cacggcgacg acgaccagat agtacccata gtcgcctctg ccctcgcctc
      481 gtccaagctg gtcaagaact cgaccctgaa gatctacgaa ggtggggacc acggcttgac
      541 ggacacacac aaggatcaac tgaaccgaga cctgcttgct ttcttactgc gaggcaccca
      601 ttagcagagg ttgccgtatc tctaggccaa cttcggtctg atccactgga ttcgcggaga
      661 cgagcttcgc ttgtggagat cgaacgacac tgcaattcgg agagaggaga agacacggat
      721 aacctgccag gcgcacagat cttcgacgta ttccccgcac cgacctcacc tgtagattgg
      781 aggatcgcat gaccagcgag aacaaccaca tcgaagccgc cacagacgct gctgccccgg
      841 acacggtcgt gttcatccac gggctgtgga tgacaccacg cagctgggag aagtggacag
      901 agcgttacga ggctcggggc tacaagacca tcgcaccggc ctggcccggc atggagggcg
      961 aagtcgaagc gctaaacgcc gatccttccc cgatcgccga actggacata gaccagatcg
     1021 tcgaccacta cacgcgcatc atcggtgagc tggacaaacc gccgatcatc atgggccatt
     1081 ccctcggtgg aaccgtgctg caggtcttgc tcgatagggg cctcggcgcc gcgggcgtgg
     1141 gcatagcccc cggaacggtg aaaggggtac ccgacctgcc cctgtctacc cttcgagcaa
     1201 ccctgcccgc cctgcgcaac ccattcaagc gccactctgc cgtcccgctg agcgcaaagg
     1261 agttccacta cgccttcgcg aacacgctga gccgggaaga gtcggacgag atctacgatc
     1321 gctaccacgt gccctgtgcc gtgaacgtgc tgcgggaagc cgccttcgcg agcctgcacc
     1381 gtgacgcccc gacgaaggta gacttcgcca acgacgaccg cgcgccgctg ctgtttctca
     1441 ccttcgagca cgaccacatc attccccctg aagtcggccg tcacaacgcc gagaagtact
     1501 cgggatccca ggccgttacc gagtttcagg agtaccttgg gcggccccac ttccctggcg
     1561 cacccggcta cgaggaggtc gccgaccacg ccatcgagtg ggccgcagaa cacgccgtag
     1621 cccgcgcatc ggtctgagca acgccccggt tagtgggccg ttcagatcga cggcccactg
     1681 caaccccaac atcacaccaa gaggtcggga cggcaaacca atgccccggg gcacgacacc
     1741 aggaggcacc ccgatgctgt atagaaacgt agccgacggc gttcatcgga tcggcgaaca
     1801 ttttgtcaac tggtacctcg tagaggagaa cggccacgtt tgcgtggtgg atgcgggcct
     1861 gccctcgtcc tggagctctc tgttggaagc cctgcacgcg atcggtcgca agccccacga
     1921 tatcgaggga ctcgtgctca cgcatgctca tttcgaccat ataggtttcg ccgaaagggc
     1981 gcgggtggaa ttcggcgtcc ctgtgtgggt tcacgagaac gacgcgcccc tcacgaagcg
     2041 accctggctg tacatgaccg aacgcagccc ggtctcctat ctgggccgca agaacctgcc
     2101 catagtgtcc tctctcatcc gggccggagc actacgcgtg ggacctattc gggaggtacg
     2161 gcggtacggt aacgagggaa cgctcgatgt gcccggatct ccaaaggtct tgttcacgcc
     2221 tgggcacacc ctgggccact gtgctctcca ctttcccgaa cgtgatgttg tcgtcgcggg
     2281 cgacgctctg gttactcacg atccctacac ggacactcgt ggcccccgga tcgtggcgcg
     2341 cggggcgacg gccgatagcg agcgggcact ttcctcgctc gaattactgg cggacacgga
     2401 cgctaggatg ctactacccg gccacggcga gccctggaca gacggtgccg aacgggccgt
     2461 gcgcgaggcc ttgcgcgtgg gcgccctgta gtcgtcgcaa gggggagcgt tgccgcccgc
     2521 tcggaagcac cggcggctgt ctctgcggtg ccatccggta tgaggaggtg cgcgggccgc
     2581 tgcgtgatgt ggtcgcctcc tgccactgct cgcagtgcca ccgcaccagc tcttcagtag
     2641 ggtgttgtgg cggtacagtc atcccagagg cctgtctcct gctcctctgc tctttcgcgc
     2701 aggcgttcga actcgcggcg gtaggcgtgg gagaaggtgg tggtcctggc gtaacccagt
     2761 ttcaggagat cctcgttgaa ggggtgctcg taggagccgt tgtggtcagc gtcgaggtag
     2821 aggtaggcga gggtgcggcc gtaggcgtcc tccgagtctc ctacgcgcgg tatctccatc
     2881 ctgaccagcc tcttcgaaag ggtcttcttt gtgtaggcgc tcgccttttt gccgtagcag
     2941 cctataggtt ggtctggtgc cacggtctcc ggggtgtcta cccctatcag cctcacgtgc
     3001 tggagctggc cgtagacctc tactgagacg gtgtcgccgt ctgttacctt ctggaccttg
     3061 ccgtagaccc ggtccgaacc gcccatgcgg tagatgaggc cgtcgaagcc tgccctggag
     3121 acgggataga agaggagcgc gagcaatagt gccacggcga cgaagacgcg ttcggaggcg
     3181 gagcccgaca cgacgcgata ctggtccctc ccgggtaaca cggctatagc ctgactgggc
     3241 cagaagagct gggcgccacc caacgacatc atgtcggcga agaggtgcga gccgtagccc
     3301 acgaacgcgg caacggcgag ggccggctgg tcagcgaccc accagagcgg caggccgagg
     3361 gctgtcgtgc agaagagcag ggccagtagg gaatgaaaac agctgcccgg cgcgaggagg
     3421 cgacggagtt cctgcggcac gcttccaagc ctgtgaccga gccagctctt cgggtcgtcg
     3481 gcgtcgggta ggatgctcgt cgccgcgacg acgagcaccg ccgaggtgcc gtagtggacg
     3541 tcgaggacgg cggaggtggc gaaccagcaa cactcggcga agacgatgtg ggtggccacc
     3601 ttcacggtta cgctagtccc ttgggcgctg catcctggag aaaaaaggcc tcaaaccgta
     3661 catcacggcg aacacgatgc cggccaggat gtagaggtcc tggtcgtcga gacccagaga
     3721 gacgtaacgc accgccatcg ccagggctgc gaggacgaag atggccggcg tcatgtcgat
     3781 gtagcgcttt tcgtcctcca gcccgagctc ctcgacctcg gcacccagcc tctcgagcgc
     3841 ggtgcgtcct gcgccggcca ggcgtccctg gacttcgacc cgccggcctt tcaggacggg
     3901 acgcagtacg tgctcgatct cccccatcga ggagcgatag agcgccctct ggaatccggc
     3961 ctcatccacc tcgcgtcccc gcacctgacc gagtttcctg gggtctatct caccagcccg
     4021 ccacgcggcc aacgcgaggt gcacgagcgc cgccttcctc gcaccggcgg gtatcgtaag
     4081 ggtatctcgg tcgtcatctg gccgtggcag acgaccggtc tccctctccc gggaagagcc
     4141 ggggccccga cgtccccggt cagcctgcct cgtgtcgaag tccacgtccc ttgccttcta
     4201 ctctcttgcg cgtggggatc cggcgcgtcc ttccgccctt tggcgcatga ggtgagagag
     4261 tatctcctcc aactccaacg ctccggtgcg gagtacgcct atgttccggg cacgcagcgc
     4321 ctctgccgtc tccccgggcg aatcgcttac gatacgtacc ggatcatcgc tatccagctc
     4381 gaccatgcct ggaatatcgc cctccggcgc cctctccggc cagaaggcgg tccacctctc
     4441 ctgcagggcg cccgtctcga agagcccgag cagctcccca tccaccagga acgctatgtg
     4501 gtcagcgatg agcctgacat cctccatggc atgggtcgcg aacacgaccg ttctgttcct
     4561 gtgccgctcg tcggctatga agtgcgagat gcccgcaagg acccggcttc gggcgaaggg
     4621 gtcaaggccc gccgtaggct cgtccaagac gagaagctcg gagccggccg ccagggccag
     4681 cgcaacaatg aggagccgct gcgtgcccga ggagagtgcc tcgaagggcc tgtcctcctc
     4741 tatctccgta cccgagaggg gcacttcgta ggccttctgg tcccatcctg gatagaagta
     4801 ggatacgagc tcgcgcaggg tcctggggct caggtcttcg tagcccgtgg cgcgctcggg
     4861 ggcgaagcct atcttctgtt ttatggctac ttcgtcctcg ggaaaagcac ccccgaagag
     4921 gctgacctca ccggagtcag gtgggacgag gttcatgagc atcttcagga gggtgctctt
     4981 gccgctcccg accggcccca cgaccgccac cacgcagccg ggctccacgt cgaggtcgat
     5041 cgggccaagc tcgaaagccc cgtaggactt gctgacgtct ctgagcgaga tgatcggtcc
     5101 ccgggtcata acctgtcccc ccctgtctcg gtctggtccc cttgcaaggt ttcgtctagc
     5161 acggcgtgta gctcatcccc ggtgagaccc gcgcggcggc cggtcccgac ggcctcagcg
     5221 aaggctgcct ttacggcctc gtggcgcctg gctctcaact tctcctcggt aatctccgcg
     5281 accacggtgc ccctcccctg cctggtcagg a
//
LOCUS       DBNGVD010000256         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3519117, whole genome shotgun sequence.
ACCESSION   DBNGVD010000256 DBNGVD010000000
VERSION     DBNGVD010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3519117"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2090
                     /locus_tag="AC16CM_07030"
     CDS             249..2090
                     /locus_tag="AC16CM_07030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3139618.1"
                     /translation="MTVPLIPREVLFGNPERVGPRLSPDGERLAFLAPKDGVLNVWVG
                     AVGAPLSDFEPVTDDRRRGIRVCFWAEDNRHIVYLQDEGGDENWRVHAVDASTHEDRD
                     LTPFDGVQAQILDKSRHFPDTLLVALNRESPERHDVYRLSISTGDLELAAENPGNVAG
                     WVADASFEVRAAMAATQEGGFDLLLRDGEEAGWRRLFGWDKEDALSSAPLGFTEDGAK
                     LYLLDSRDANASRLTLLDLQSGDTEVLVEDPRYDVGQVLTNPETHEVQAAAVERARTD
                     WVVLDDAIDEDFEKIAGLGRGDFAVASRDRADENWLVAFTADDGGASYYSYDRKGKTG
                     EHLFDARPDLAGYDLARMKPISFTSRDGLTIEGYLSLPPESSDGPVPMVLDVHGGPWA
                     RDGWGYNPEAQWFANRGYACLQVNYRGSTGYGKEFLNAGNKEWGARMHDDLVDAVGWA
                     VEEGVADPERIAIYGGSYGGYAALAGATFTPDLFRCAVDIVGPSSLITLINSIPPYWK
                     PLLATFHERVGNPETEREFLESRSPLFFVDRVKIPMLIAQGANDPRVKQAESEQFVAA
                     MQEKGIDHEYMLFEDEGHGFARPENRLKFYAAAESFLARHLGGRTEE"
     gene            complement(2182..2334)
                     /locus_tag="AC16CM_07035"
     CDS             complement(2182..2334)
                     /locus_tag="AC16CM_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139619.1"
                     /translation="MEDRGEVCLAEEVERLRKLGLSDEEVAGRLGVDVSWVESLVSPG
                     DGEPQA"
BASE COUNT          483 a          834 c          767 g          346 t
ORIGIN      
        1 cgcaccttcg ccgacggcgc catccgagcc gcaaccttcg ccgccaacgc gcgtcccggc
       61 ctgtactcaa tgaaagacgc cctcgggacc tagaagccag caagcgctaa actcaacagc
      121 gtgaaggcac gagaaagctg acaaggcgtg aagcgccgtg ccgaaagcga gagccttcag
      181 gctcgaagcg ttgccgacaa gcgaggccct aagggcgaaa cgtgctgtga caaccagagg
      241 gaggaaccat gaccgtgccg ctgataccgc gagaggttct gttcggaaat ccagagcgcg
      301 tcgggccacg actctcccct gacggggagc gcctggcctt tctcgcgccg aaggatggcg
      361 tgttgaacgt ctgggttggt gccgtcgggg ccccactctc ggacttcgag cccgtgaccg
      421 acgatcgcag gcgcggcatc agggtctgtt tctgggctga ggacaacagg cacatcgtct
      481 acctccagga cgagggcggc gacgagaact ggcgcgtgca cgccgtagac gcgtccacgc
      541 acgaggaccg cgacctgaca ccgttcgacg gcgtgcaggc ccagatcctt gacaagagcc
      601 gccacttccc ggacacgctg ctcgtggcgc tcaacaggga gagccccgag cgccacgacg
      661 tctaccgcct gagcatctca accggcgacc tggagctcgc cgccgagaac cccggcaacg
      721 tcgccggctg ggtcgccgac gcgtccttcg aggtccgggc cgccatggcc gccacccagg
      781 aaggaggctt cgaccttctc ctgcgggacg gcgaggaggc cggctggcgc aggctcttcg
      841 gctgggacaa ggaagacgca ctatcaagcg caccccttgg ctttaccgaa gacggcgcga
      901 agctatacct ccttgactcg cgcgacgcca acgcctcgcg cctgacgctg ctcgacctgc
      961 agagcggcga tacggaggtg ctcgtagaag acccgcgtta cgacgtcgga caggtgctga
     1021 cgaaccccga gacccacgag gtacaggccg ccgccgtcga gcgggcgagg acggactggg
     1081 tggtgctgga cgacgcaatc gatgaagact tcgagaagat agcgggcctg ggccgcgggg
     1141 acttcgccgt ggcgagccgc gaccgggccg acgagaactg gctcgtcgcc ttcaccgccg
     1201 acgatggcgg ggcctcgtac tactcctacg accgtaaagg caagaccgga gagcacctct
     1261 tcgacgcccg tcctgacctc gccggctacg acctcgccag gatgaaacct atctctttca
     1321 cctcccgcga cggcctgacc atagagggct acctctccct gccgccggag agctcggacg
     1381 ggcccgtgcc gatggtgctc gacgtgcacg gaggcccgtg ggccagggac gggtggggct
     1441 acaacccgga ggcccagtgg ttcgcgaacc ggggttacgc ctgcctgcag gtcaactacc
     1501 ggggatcgac ggggtacggc aaagagttcc tcaacgccgg caacaaggag tggggcgcca
     1561 ggatgcacga cgacctcgtc gacgcggtcg gttgggccgt cgaggagggc gtggcagatc
     1621 ccgagcggat agcaatctac ggcgggtcct acggcgggta cgccgcgctc gccggggcca
     1681 ccttcacgcc cgacctcttc cggtgcgccg tcgacatcgt cggcccgtct agcctgatca
     1741 ccctgataaa cagcatccct ccatactgga agcccctgct cgccaccttc cacgagaggg
     1801 tcggcaaccc cgaaacagag cgggagttcc tggaatcgcg ctcgcccctg ttcttcgttg
     1861 accgtgtaaa gataccgatg ctcatagccc agggcgccaa cgacccgcgc gtaaagcagg
     1921 cagagtcgga acagttcgtc gccgccatgc aagagaaggg catcgaccac gaatatatgc
     1981 tcttcgaaga cgagggccac ggcttcgccc gacccgaaaa ccggctgaag ttctacgccg
     2041 ccgccgaaag cttcctggcc agacacctgg gaggccgaac cgaagaataa cgcagggcgt
     2101 ctggacaacc cgccaaagac ggacgtacgg cgccattacg caccaacggt gtaggggcag
     2161 ttcgcgaacc gcccgactac gtcatgcctg gggctccccg tccccgggcg agacgaggct
     2221 ctccacccaa ctcacgtcga cgcccagcct gcccgccacc tcttcatcgg agaggccgag
     2281 cttcctgagg cgttcgacct cttcagcgag acagacctcg ccccgatctt ccaaaccttt
     2341 cctccgatcc tctagctaac cctcgcgcga gcggcggccc cgcatgatca ggaccagaga
     2401 aacgaccagc agcggggcca acagagccgc
//
LOCUS       DBNGVD010000257         4203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4613034, whole genome shotgun sequence.
ACCESSION   DBNGVD010000257 DBNGVD010000000
VERSION     DBNGVD010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4613034"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..983
                     /locus_tag="AC16CM_07040"
     CDS             72..983
                     /locus_tag="AC16CM_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139620.1"
                     /translation="MARGLKKSYAGFEAVKGVDFEISGGECYGFLGPNGAGKTTTMKM
                     IYGAVIPSGGELSVAGLDVYHNERAIKRRIGVVPQENNLDDELGVRENLLIYGRYYDL
                     PKKTVLQRTDELLDFVQLADKVDARVEQLSGGMKRRLLIARALINDPEIVVLDEPTTG
                     LDPQARHLVWDRLRALTDEGKTLVLTTHYMEEAARLCDRLVIMEGGRIIAGGTPKQLV
                     REHVSPQVLEFRASAGVIDGLIPLVDPVADAVERTGDEVLLVYTEEAEDLVVRVQRAD
                     VDLQNTVQRQAGLEDVFLRLTGRSLIE"
     gene            983..1780
                     /locus_tag="AC16CM_07045"
     CDS             983..1780
                     /locus_tag="AC16CM_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139621.1"
                     /translation="MSTIRIPSPRGAFRVWQRNVTIFRKFWVSGLFPNFVEPLIYLAA
                     LGLGLGTFIQQGGISGESYVQYIAPGLLASNAMFAASFESTFNTFVKLRFDKIYDAII
                     TTPVNAEDVIAGEYLWAGTRAALYGTGFLGMLMVLGTAFGEPLITSWWAVFIPPMLLL
                     IGIMFSVLGTLFTSLISHIDLFAYYFTLVVTPLFLFSGIFFPVQNFPAPVPQVAWFTP
                     LYHAVNVCRELATGPSPWVLVDVAWIVVFTGAIALVPIQIMRRRLIG"
     gene            1807..2430
                     /locus_tag="AC16CM_07050"
     CDS             1807..2430
                     /locus_tag="AC16CM_07050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139622.1"
                     /translation="MEAEQFTVRFAEPEDDPVIATLVVEGFLDKFRPVFGSRMDRSLK
                     IMERWVDLEHLSGGVTSLVIEGYSTSELAASVGVRTEASREDVLARGLWKALTRNLGF
                     LRAVWATTLLSYPRYSSKPSEAYVERLVVSPSFRRLGMGRRLLSAAEDLARDRGKEAI
                     GLHVTADNLPALRLYESEGYREISRQRSLMTSHFLNIREWLYLKKEL"
     gene            complement(2738..4009)
                     /locus_tag="AC16CM_07055"
     CDS             complement(2738..4009)
                     /locus_tag="AC16CM_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139623.1"
                     /translation="MSIVLIVVLVLVVLVVGVSVFLGRATSEVESARDTEYLELEGVL
                     VRYNVVGGGPAVVLVHGWLTSSRLWEQLAGRLAQRFTVYTLDLSGFGESDKPAAGYGI
                     RNGSRLLYAFCAHFGLTRANVIGHDFGGAMAVKLAADHPDVVGRLVIVSTPADEDQID
                     VPTLLWLATLPLVGPLFYALGRAARPVRRLWMRPFVADPDDLTEEVVDDAGRSTPSAV
                     AKTLSIGKREISRGRLARQARIIKIPLLVVAGEQDQIVDPQSVGVWAGGVDRAEIALI
                     DECGHLPMIERTAEFNAQILAFLTGDPRYLDFIPPARTATPAGEQPQIEEVPDQAEPE
                     AAPVDIAPVPPPADDTSDLPPAAPDLDRAGGPKVVRKRNGVYPARTRPPDPEGPYPIE
                     ETPQRRKPSDDHFLTDLPDDLFNWPERRREE"
BASE COUNT          733 a         1345 c         1355 g          770 t
ORIGIN      
        1 tgagaggttt gcggttgaac acggagacga agagcaacgt cgtgaaccgg tctggaagct
       61 catctaccct tatggcgcga gggctcaaga agagctatgc cgggttcgag gcggtcaagg
      121 gcgtcgactt cgagatctcc ggcggcgagt gttatggttt tctcggcccc aacggcgccg
      181 gcaagaccac tacgatgaag atgatctacg gtgcggtcat accctcgggc ggcgagctct
      241 cggtggccgg cctcgacgtg tatcacaacg agcgcgcaat aaagcgccgc ataggggtcg
      301 tgccccagga gaacaacctc gacgacgagc tcggggtgcg cgagaacctg ctcatctacg
      361 gcaggtacta tgatctgccc aagaagacgg tcttgcagag gacggacgag ctcctcgatt
      421 tcgttcagct cgccgacaag gtggacgcca gggtcgaaca gctctccggg gggatgaagc
      481 gcaggctact catcgcgcgg gccctcatca acgacccgga gattgtggtg cttgacgagc
      541 ccacgacggg tctcgacccg caggcgcggc atctcgtctg ggaccggctc agggcgctca
      601 cggacgaggg caagacgctc gttctcacca cccactacat ggaggaggcc gcccgactct
      661 gcgaccgcct cgtcatcatg gagggaggcc gcatcatcgc ggggggaacc ccgaaacagc
      721 tcgtgcggga gcacgtcagc ccgcaggtcc tcgagttccg cgcgagtgct ggcgttatag
      781 acggcctgat cccgctggta gacccggtcg ccgatgcggt ggagcgaact ggcgatgagg
      841 tcctactcgt ctacaccgag gaggcggaag atctcgtagt gcgggtgcag agagccgacg
      901 tggacctcca gaacaccgtg caacggcagg ccgggctcga ggacgtgttc ctgaggctga
      961 cgggaaggag cctgatcgag tagtgtcgac catacgaata ccatcaccgc ggggcgcctt
     1021 ccgggtctgg cagcgcaacg tcaccatctt ccgcaagttc tgggtcagcg gcctttttcc
     1081 aaacttcgtc gagcccctca tctaccttgc ggcgctcggc cttgggctgg gcacgttcat
     1141 tcagcagggc ggcatttccg gcgagagcta cgtgcagtac attgcgccgg ggctgctggc
     1201 gagcaacgcc atgttcgccg cctccttcga gagcaccttc aacaccttcg tcaagctcag
     1261 gttcgacaag atctacgacg ccatcataac cacgccggtg aacgccgagg acgtgatcgc
     1321 cggcgaatac ctgtgggccg ggacccgggc cgccctctac gggaccggct ttctcggcat
     1381 gctgatggta ctcggcaccg cgtttggcga gcccctgatc acctcctggt gggcggtctt
     1441 catcccgccc atgctcctgc tcatagggat catgttcagc gtgctcggaa cgttgtttac
     1501 cagcctgatc agccacatcg atctcttcgc ctactacttc acgctggtcg ttacgcccct
     1561 gttcctcttc tccggcatct tcttcccggt tcagaacttc ccagctcccg tcccgcaggt
     1621 ggcgtggttc accccgctct accacgcggt caacgtgtgc agggagctcg ctactggccc
     1681 gtccccctgg gtgctggtcg acgtggcctg gatcgtcgtt ttcaccggtg ccatcgcact
     1741 cgtgcccatc cagatcatgc gtcggcgtct catcggctga cgggcaaact ctggtaggat
     1801 ggcgcggtgg aagccgagca gttcaccgta cgcttcgccg agccggagga cgatccggta
     1861 atagctaccc tcgtggtcga ggggttcctc gacaaattcc ggccggtctt cggaagcagg
     1921 atggaccggt cgctcaagat catggagcgg tgggtcgatc tggaacacct ctccggcggc
     1981 gtaacatccc tggttatcga gggctactcg acctcggagc tggctgccag cgtcggcgtt
     2041 agaacggaag cctccaggga agacgtgctg gcccgaggcc tgtggaaggc cctgacccgc
     2101 aacctcggct ttctacgtgc tgtgtgggca acgaccctgc tctcttaccc caggtactcc
     2161 tccaaaccct cggaggccta tgtagagcgc cttgtggtct caccatcctt caggcgcctg
     2221 ggcatgggca ggcgcctgct ctccgcggcg gaagacctgg ccagggaccg gggcaaggag
     2281 gctatcggcc tgcacgtaac cgccgacaac ctgccggccc tccgcctcta cgaatccgaa
     2341 ggctaccggg agatctcccg acagagatcc ctgatgacct cccacttcct gaacatccgc
     2401 gaatggctgt atctgaagaa ggagctgtag ggagcacgct ccggctgacg cctacgcttt
     2461 ctcagcacgg cccttcgggc ctttcagcac gctcccttcg gtcgctttca gccagtcgtc
     2521 tctgttcgtg acggtgatcc agatgtggag ccttcctacg tatcgtcacg ttctgcgggt
     2581 aaagtgcagg ccgcagcggg atctggccga aagcgagcct ggcgaagccg ggcgaagcgt
     2641 tgctgacaag cgaagggcgc tagcccgagc gtgctgacaa acggaggccg acaggccgta
     2701 gtgtgctgag gagcggagcc cgaagggcgt agcgtgctta ctcttcgcgc cggcgttcgg
     2761 gccagttgaa gaggtcgtcg ggtaggtcgg tgaggaagtg atcgtctgag ggcttgcgcc
     2821 tctggggggt ctcctcgatc gggtaggggc cctctgggtc gggaggtcgg gttctggccg
     2881 ggtagacccc gttccgctta cggaccacct tcggtccgcc ggcccggtcg agatctggcg
     2941 cggccggggg caggtccgag gtgtcgtcag cgggaggagg aaccggggcg atgtcaacgg
     3001 gtgctgcttc gggttcagcc tggtcgggga cctcttctat ctgaggttgc tctccggcgg
     3061 gtgtggcggt ccgggccggc gggatgaaat ccaggtagcg agggtcgccg gtcaggaaag
     3121 cgaggatctg ggcgttgaac tcggcggtgc gttctatcat gggcaggtgg ccgcactcgt
     3181 cgataagggc gatctcggcc cggtcgactc ccccggccca cacgccaacc gactgcgggt
     3241 ctacgatctg atcctgttct ccggcgacga cgagcagtgg gatctttatg atcctggcct
     3301 gcctagcgag cctgccacgg gagatctcgc gtttccctat gctgagggtc ttggcgacgg
     3361 cggacggcgt ggacctaccc gcgtcgtcga caacctcctc ggtcaggtcg tcggggtcag
     3421 cgacgaaggg gcgcatccac aaccggcgga ccggtcgcgc cgcgcgcccc agggcgtaga
     3481 acaggggtcc gacgaggggc aaagtagcca gccagagcag ggttggcacg tcgatctggt
     3541 cctcgtcggc cggggtagac acgataacga gccgtccgac cacgtccggg tgatcggcgg
     3601 ccagcttcac ggccatggcc ccgccgaagt cgtggccgat aacgttggcg cgcgtcaggc
     3661 cgaagtgggc gcagaaggcg tagagaagcc tgctcccgtt ccgaatgccg taccccgcgg
     3721 cgggcttgtc cgactccccg aaaccgctaa ggtctagggt gtagaccgtg aaccgctggg
     3781 ccagacgccc agcgagttgc tcccagaggc gcgaagaggt aagccacccg tgcaccaaca
     3841 ccaccgcggg cccaccaccc acgacgttgt accggaccag aactccctcc agctccaggt
     3901 actctgtgtc gcgggcgctc tctacctcgc tggtcgcccg cccgagaaag accgaaaccc
     3961 cgacaaccag cacaaccagc accagaacta ctatcaaaac gatgctcatg tgcgcatgat
     4021 agcgcaaaag agcacgcttc ggcttcgcct acgctcctca gcacactccg gcctgcggcc
     4081 tccgtttgtc agcacgctcc agcctgcggc tgtcgcttgt cagcacgctt cgcccggctt
     4141 cgcctggggc tcgctttcag ccagatcccg ctgcggcctg caccctgccg ctcttgagat
     4201 tcg
//
LOCUS       DBNGVD010000258         3455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3701799, whole genome shotgun sequence.
ACCESSION   DBNGVD010000258 DBNGVD010000000
VERSION     DBNGVD010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3701799"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16CM_07060"
     CDS             complement(<1..529)
                     /locus_tag="AC16CM_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3139624.1"
                     /translation="MSRVVVVGGGLGGLASAALLGRAGHEVTLLEANPWLGGKSRRIE
                     LDGRRMDTGPSLLTFPGVWDELIRRWDAAGGNPGEASTVAGLKLRRLPEVGRYYFRGE
                     STSLPVEKGHPWHEPWERFAGVHGGLGPEVTSLLTSDPTDRRTLPALVRLLKVYGPKL
                     TTRSYLDGLSWLPAGL"
     gene            complement(526..1368)
                     /locus_tag="AC16CM_07065"
     CDS             complement(526..1368)
                     /locus_tag="AC16CM_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3139625.1"
                     /translation="MILRLFHISRPVLWINTLGPAVVGMWLAGDLWRWEAVPILLWLT
                     LPFNLLIYGVNDVFDQETDARNPRKGTLEGARIDPSEVRPIALGVIASNIPFLAYFGL
                     FVPPSAVAWMLLYALLFVGYSVPPARFKARPYLDSLSNAAYAFPLVFVPLGLGEGPVW
                     PAAIGLMAWSAAKHTFDAVQDVDEDRKVGITTTAVKLGPGGVVLWSGAFWALATVCFA
                     LVNLPVAVVNAAIAGTLLYFLHENPTPRTGHRLYRYSIAFPYLAGTVAGVQLVAALSL
                     GAYP"
     gene            1481..2227
                     /locus_tag="AC16CM_07070"
     CDS             1481..2227
                     /locus_tag="AC16CM_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR3139626.1"
                     /translation="MVLTRLRRATPRLLVAACALAVVAAFFAVRFPDVPGASIGSYVS
                     TFLIALPSAVALFGYLGPSRALLSLLVLSGFAYGIESLGVVTGFPYGHFFYGEALGPT
                     LAGLVPFLLPVSYVPLVVGAVAATWGAPRPLHIAFSALILVWMDTILDPGATLLGFWV
                     WPGGGAYYGVPASNFAGWLLSGALAAALLLTTGRWSDSPHQGLLDSAVISTAFWTAVA
                     TFSGLVLPALLGITLLVFLLARRAYLPASK"
     gene            2387..2608
                     /locus_tag="AC16CM_07075"
     CDS             2387..2608
                     /locus_tag="AC16CM_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139627.1"
                     /translation="MIQEAGTGEVGIRREGSAYRSGWEDGRFGQVQTFATNNSLAVWT
                     EFPDRLSYYRGHREGRRIRNMLGSDVSPV"
     gene            complement(2773..3141)
                     /locus_tag="AC16CM_07080"
     CDS             complement(2773..3141)
                     /locus_tag="AC16CM_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3139628.1"
                     /translation="MRRLRETREEGFTLIELVVVIAIIGILTAIAIPNYLGQQQEAKD
                     AAAMAQLRAAATSQDLYHAKQNAFAGDATELEAYGFRQGGQPVAVMAADASTYCMQAA
                     GGTVNFKITQDSGRPVPGTC"
BASE COUNT          594 a         1118 c         1083 g          660 t
ORIGIN      
        1 tcaaccccgc cggcaaccac gagagaccat cgaggtagga gcgggtcgtc aacttcggcc
       61 cgtacacctt gaggagccgg accagggcgg gtagtgtcct gcgatctgtg gggtcggacg
      121 taagcaagct cgtgacctcg ggcccgagac caccgtgaac gccggcgaac cgttcccagg
      181 gctcgtgcca gggatgcccc ttctccaccg gcagcgaggt gctctcgccg cgaaagtagt
      241 accttcctac ctcgggcaac cgcctcagct tcagcccggc gacggtggaa gcctctccag
      301 ggttacctcc cgctgcgtcc cagcgccgta tgagctcgtc ccacacgcca gggaaggtaa
      361 gtagcgacgg ccctgtatcc atccgtctgc cgtcgagctc gatgcgccgg ctcttaccgc
      421 caagccacgg gtttgcctcg agcaacgtta cctcgtgccc ggcacgcccc aggagcgccg
      481 cggaggccaa cccaccgagg ccaccgccaa ccaccacgac gcggctcatg ggtaagcccc
      541 cagcgacaac gccgcgacga gctgcacgcc ggccaccgtg ccggccaggt aggggaaggc
      601 gatggagtat ctgtagagcc ggtgccccgt gcgtggggtg gggttctcgt ggaggaagta
      661 caacaacgta ccggctatcg ccgcgttcac gacagctacc ggcaagttga cgagggcgaa
      721 acacaccgtg gccagcgccc agaacgcccc gctccacagg acgacgccgc caggtcccag
      781 ctttaccgcg gtcgtggtga taccgacctt cctatcctcg tccacgtcct ggacggcgtc
      841 gaaggtgtgt ttggccgcgc tccacgccat caatcctatc gcggccggcc agaccggtcc
      901 ctccccgagg ccgagcggta cgaagaccag cgggaaggcg taggcagcgt tgctcaaaga
      961 gtcgaggtag ggcctggcct tgaagcgcgc gggcggcacg gaatagccca cgaagagcag
     1021 cgcgtacagg agcatccagg cgacggcgga gggtggcacg aacagcccga aatatgccag
     1081 gaacgggatg ttagacgcga tgacgccgag cgctatgggc cggacctccg agggatctat
     1141 cctggctcct tccagggtac ccttgcgggg attccgggcg tcggtctcct ggtcgaagac
     1201 gtcgttgacg ccgtagatga gcaggttgaa gggcaacgtc agccagagga ggataggtac
     1261 tgcctcccag cgccagaggt cacccgcgag ccacatgccc accaccgcgg gcccgagggt
     1321 attgatccag agcacgggcc gcgagatgtg gaagagacgc aggatcacgc gtctaatata
     1381 cagctacggg cttcgagcgg tcagtaatgt agctatcagt ggtgggcggt cagcttctag
     1441 cctccggttt cgggcggatc gcgtgccgct gtagaatcgt atggtgctta ccaggcttcg
     1501 ccgcgctacg ccccgactcc ttgtggccgc ctgcgccctg gccgttgtgg ccgctttttt
     1561 cgccgtccgc tttcccgacg tgcccggcgc gagcatagga tcttacgtct ctacgttcct
     1621 gatcgcgctt ccctcagccg tggcactttt cgggtacctc ggtcccagca gggcgcttct
     1681 atccctgctc gtgctctcgg gttttgccta cgggatcgaa tccctcggcg tcgtgaccgg
     1741 gttcccatac ggacacttct tctacggcga agcgctggga cctaccctgg ccgggctcgt
     1801 tccctttcta ctgcccgtgt cctacgtgcc gctcgtggtc ggggccgtgg cggccacctg
     1861 gggcgccccc cgaccactgc acatcgcctt ttcggccctg attctcgtct ggatggacac
     1921 gattctcgac cccggcgcca ccctcctggg cttctgggtg tggccgggtg gcggggccta
     1981 ctacggcgtg ccggcgagca acttcgcggg atggctcctc tccggcgcac tcgccgcagc
     2041 cctgctcctc accaccggcc ggtggtccga ttccccacac cagggcctgc tcgacagcgc
     2101 ggtgatctct accgccttct ggacggccgt ggccaccttc tcgggtctcg tgctcccggc
     2161 cctgctcgga atcacgcttc tcgtcttcct cctcgcccgc cgcgcatatc tgcccgcctc
     2221 gaagtagtta cgataggcac aatcttttcc accgattcat cgaaacggca ccagatatag
     2281 cagtcccgta acttttgttc gattttatta agctgacctc ttacatttgt gaatccattg
     2341 tgtttacata atgctcgtaa gatacacccg tcacaggagg tctgtagtga ttcaagaagc
     2401 tggcaccggg gaggtgggga tccgccgtga gggttcagcc tacaggagtg gctgggagga
     2461 tggacgtttc gggcaggtac agaccttcgc caccaacaac agcctggcgg tgtggaccga
     2521 gttcccggac aggctctcct attatcgggg gcaccgcgag ggacgtcgca taaggaacat
     2581 gctggggagc gacgttagcc cggtctgagg gtaacgggcg gttcttagct atcagattct
     2641 ggcccttatc gctgcctacg ggtggtcggc agcgataagg gccgacggct ccccctccag
     2701 gttcgccctc ggccctcggg gcatgttaac caaagtgctg ggtgagtgcc aaccggtcga
     2761 atcccggtag tcctagcacg taccgggtac gggtctaccc gagtcctggg ttatcttgaa
     2821 gttcaccgtg ccgcctgcgg cctgcatgca gtatgtgctt gcgtctgcgg ccatcaccgc
     2881 gaccggttgt cccccttgcc tgaaaccgta ggcctccagt tcggtggcgt cacccgcgaa
     2941 ggcgttctgt ttcgcatggt acaggtcttg agaggtggcg gcggcacgaa gttgcgccat
     3001 cgcggcggcg tccttcgcct cctgctgctg gccgaggtag ttcggtatcg cgatggccgt
     3061 cagtatcccg atgatcgcga tgaccacgac cagctcgatc agcgtaaagc cctcctcgcg
     3121 cgtctcacgt aacctccgca tgtttccgcc tctgttgtcc cggggtctgc accgagtctt
     3181 cggcggtaga gagcgccagc tttagttgca gaacaggcaa cacgtgtcag aacctcggtg
     3241 gacaggcttt gcagagggtt ggcaagcgcc gcgggggtga gggacgccgg ccgcaagaaa
     3301 aatcatccga aaacgaggtg gggtacaata cggggcagga tgggaatcac aacctggagg
     3361 ctgttgatag tggggctggt gctacttgcc ggtctcgtct cgcgtgtcac ctacgagcag
     3421 ctgatccacc cggaagagcc agccgtggcg caatc
//
LOCUS       DBNGVD010000259         4969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2244224, whole genome shotgun sequence.
ACCESSION   DBNGVD010000259 DBNGVD010000000
VERSION     DBNGVD010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2244224"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="AC16CM_07085"
     CDS             complement(<1..516)
                     /locus_tag="AC16CM_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HIR3139629.1"
                     /translation="MEALTRAATVGMSREAPPEGGLPTDGLLAGAQRNPERELLLRAG
                     MLAVYDAAGRRAKTGVGMPATAPEETMPACSARVAEILRPLLAGQRPEILREALDRLH
                     LAGMRVPHALLPAALGVQQQGLRPAVAHVLGERGSWLAAQNLDWEWAVATRGSVDDES
                     TWEEGPPEERIV"
     gene            complement(516..1820)
                     /locus_tag="AC16CM_07090"
     CDS             complement(516..1820)
                     /locus_tag="AC16CM_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3139630.1"
                     /translation="MSVEAVAALAPDSRVAAAGRKLGVSGSWNSPGRSDGALWGECKG
                     SAVYQVRVDLSDLAVKCSCPSRKHPCKHGIGLLFLNLDSPLPAVPAPDWVTDWLARRA
                     AGPKRRKSAAKRGGTVSDPEAKAKRAEKRLSRVAGGLDALDLWMEDLVRDGLATAGTR
                     PASFWETQAKRMVDAQAPGVAARLRHLSALPNSSPDWTGRLLDGLGRLALLSEAFRRL
                     DSLDEPLAESVRAEVGIPLPQEAVLDRGERVSDDWIVLGQRTDDEWRLRTRRTWLLGG
                     ESRRYAMILQFAASGAPFAEQFVSGTIVSGELAFYPGAYPLRAVVRSRAGVATRAVEL
                     PGHRTVEAFLDHASTITASQPWLEWLPATLSGVVPLLDGERWLVRDQDGGALPLSGGE
                     HWTLISISGGHPLDLVAEWEGGTLLPLGVVTGGRYRMIQGHE"
     gene            complement(2030..2818)
                     /locus_tag="AC16CM_07095"
     CDS             complement(2030..2818)
                     /locus_tag="AC16CM_07095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139631.1"
                     /translation="MKEREEVAVITGTARGIGRRAALTLAEEGYRIAVNDLDTPEETL
                     RELRAAGTEALSMPGDVSDEAAVRGMVETVLENLGRVDVLVNNAGISAIVPAEETTLA
                     DWNRTIAVNLTGPFLTCREFGKVMLGQGSGSIVNVSSVAGLLGVSDRTAYNASKHGLV
                     GLTRTLAAEWGGRGVRVNAVCPGWVKTEMDEEDQGAGGYADADIEGRVPMARFATPED
                     IARAVAFLADPVWSGFVNGHTLSVDGGWFADGSWEGLRRSKQGL"
     gene            complement(2914..3870)
                     /locus_tag="AC16CM_07100"
     CDS             complement(2914..3870)
                     /locus_tag="AC16CM_07100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3139632.1"
                     /translation="MPDKVLVAREIPEAGLRVLEPFDTTVLHERPPERDELLEAVRGV
                     SGVLSTATETMDGEVMDAAGEGLRVIANMAVGYDNVDVEAAGERGIIVTNTPGVLDET
                     TADVAFMLLLAAARRLGEAERLLRAGGWEWWGPKQLMGRDVWGKKLGIVGFGRIGQAV
                     ARRARGFGMEILYHNRSRKEEAEREIEARYMQLGDLLEAADFVSIHTPLTPETTHLIG
                     ADELDGMKPEAVLVNTSRGPVVDEAALAEALADRRIFAAGLDVYENEPEVHPKLLELE
                     NVVLAPHIGSASVETRDRMATLAAENLAAVLRGESPKTPVNE"
     gene            complement(3870..4358)
                     /locus_tag="AC16CM_07105"
     CDS             complement(3870..4358)
                     /locus_tag="AC16CM_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3139633.1"
                     /translation="MRRFDLAEAVRLREVGDYREAESLISRSLAEIPDDAEANYQMSC
                     LYDLQGREREAVPHYVKAVAGDLPEKDRRGALLGLGSTYRSLGEYPEAVEVLGQGVSE
                     FPADRAMQTFLALALYNVGEHRQAVDLLLKHLVETTSDPGIRSYEKALRFYTGRLDEV
                     WG"
     gene            complement(4361..>4969)
                     /locus_tag="AC16CM_07110"
     CDS             complement(4361..>4969)
                     /locus_tag="AC16CM_07110"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259000.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR3139634.1"
                     /translation="QYLIQKGVDSRDFNSYGSRRGNHEVMVRGTFGNIRLRNQLVDEE
                     GGYTVHLPDGEKTTIYEASLQYAEENVPLLVLAGKEYGSGSSRDWAAKGSFLLGVRAV
                     IAESFERIHRSNLIGMGVLPLQYSDGESAESLGLTGHESFDISGLADLEPGKVLTVKA
                     TADDGGVTEFEAKARVDSPVEVEYLRNGGILQTVLRQLLDEE"
BASE COUNT          788 a         1743 c         1579 g          859 t
ORIGIN      
        1 aacaattctc tcctccggcg ggccctcctc ccaggtgctc tcgtcatcca ccgatccacg
       61 ggtcgccacc gcccattccc agtcgagatt ctgcgcggcg agccacgatc ctcgttcccc
      121 gagcacgtgt gccacggccg gtcgtagtcc ctgctgttgc acgccgaggg cggcgggcag
      181 cagcgcgtgc ggcacacgca tgccggccag gtgcaggcga tccagggctt cgcgcaggat
      241 ctcgggccgc tggccggcaa gtagcgggcg caagatctcg gccaccctcg cggaacaggc
      301 cggcatcgtc tcctcggggg ctgtcgctgg cattcctacg cctgttttcg ccctcctacc
      361 cgcggcatcg tagacggcga gcatcccggc ccgcaggagg agctcccgct cggggttcct
      421 ctgtgcgccg gcgagaagac catctgtggg tagcccaccc tctggtggtg cttcccggga
      481 catcccgacg gtggcggcgc gggtgagggc ttccactact cgtgcccctg gatcatgcgg
      541 taccttcccc cggtcacgac cccgaggggg agcaatgtcc caccctccca ctccgcgacg
      601 aggtcgaggg ggtggccgcc ggagatggag atcagggtcc agtgctcgcc gcccgagagc
      661 ggcagcgctc cgccatcctg gtcgcggacc agccagcgtt ctccgtcgag aaggggcacg
      721 acacccgaga gcgtcgccgg taaccactcc agccagggct ggctggccgt tatggtggag
      781 gcgtgatcca ggaacgcctc aacggtcctg tggcccggca gttccaccgc acgggtcgcc
      841 acgcccgcgc gggacctgac cacggcccgc aggggatagg caccaggata gaaggcgagc
      901 tctcccgaca cgatcgtgcc cgagacgaat tgctcggcga agggcgcacc ggaagccgcg
      961 aactgcagga tcatagcgta acgccggctt tctccgccga gcagccaggt tcggcgggtc
     1021 ctcaggcgcc actcgtcatc ggtgcgttgc ccgagcacga tccaatcgtc ggagactctc
     1081 tcgccgcggt ccaacaccgc ttcttgcggg agcgggatgc cgacctcggc cctgacgctc
     1141 tcggcgaggg gctcgtcgag agagtcgagc cggcggaagg cttcggagag gagcgcgagc
     1201 ctcccgagcc catcgaggag cctccctgtc cagtccggcg acgagttcgg caacgccgag
     1261 agatgcctga ggcgggcggc gacgccgggc gcctgggcgt ctaccatgcg cttcgcctgc
     1321 gtctcccaga aagaggccgg cctggttccc gccgtggcga ggccatctct taccaggtcc
     1381 tccatccaca ggtcgagcgc atcgagcccg ccggcaaccc gtgagagccg cttctcggcc
     1441 cgcttcgcct tcgcctctgg atctgatacc gtccccccgc gcttcgcggc gctctttcgg
     1501 cgcttcgggc cggctgcacg cctggcgagc caatccgtga cccaatctgg tgccggcacc
     1561 gcgggtagcg gcgagtccag gttcagaaaa agtagcccga tgccgtgctt gcaggggtgt
     1621 ttgcggctcg ggcaggagca ttttacggcg aggtcagaga ggtccacacg cacctggtag
     1681 acggcgctgc ctttgcactc gccccagagg gctccgtcgg agcggccggg gctgttccag
     1741 gatccagata ccccaagctt cctacctgcg gcggcgaccc tggagtccgg cgcgagcgcc
     1801 gcgacagctt cgacgctcat gccagcgctc aagagttacc catccacctg acgcaaggcc
     1861 accagaacac agaaccagta catccaacca tgcgacgaat tctaacgacc catacgatcc
     1921 gaaaacacag ttgaaaagcc tggcacaccg tgtagagatg ctctgcagct tcgagccgtc
     1981 tcccacgagc caaaaagccg acagagctga gggctgatag ctgacagctc tacagaccct
     2041 gcttgctgcg ccgtagacct tcccacgacc cgtcggcgaa ccagcctccg tccacggaga
     2101 gggtgtgccc gttgacgaaa ccgctccaga ccgggtcggc gaggaatgcg acggccctcg
     2161 ctatgtcttc gggtgtggcg aagcgtgcca tggggacgcg gccctcgatg tccgcgtcgg
     2221 cgtagccgcc cgcaccctgg tcctcctcgt ccatctcggt tttcacccag ccggggcaga
     2281 cggcgttgac ccgcacgccc cgtccccccc actcggcggc cagcgtgcgg gtcagcccga
     2341 cgagcccgtg tttgcttgcg ttgtatgccg tccggtccga gaccccgagc aggccggcca
     2401 cggagcttac gttcactatg cttcccgaac cctgcccgag catcaccttg ccgaactccc
     2461 tgcacgtcag gaagggcccg gtcaggttta cggcgatggt cctgttccag tcggcgagcg
     2521 tggtctcctc ggcgggcacg atggcgctga ttccggcgtt gttcacgagc acgtctaccc
     2581 gtccgaggtt ctccaggacc gtctcgacca tcccgcgcac cgcggcctcg tccgacacat
     2641 caccaggcat tgacagggct tccgtaccgg ccgcgcgaag ctctcgcagc gtctcctcag
     2701 gcgtgtcgag gtcgttcacg gctatccggt atccttcttc cgcgagcgtg agcgccgccc
     2761 gccttcctat accgcgggcc gttccggtga tgaccgcgac ctcttccctc tccttcaaac
     2821 tattctcctc ctgaaacggt ttgcggaaag agcgaacccg ggatgggaag gctacaggct
     2881 gcgggcctct cttatcctgc ggcctgaagc ctcctactcg ttcaccggtg tcttcgggct
     2941 ctcgccacga agtacggcgg ccaggttctc ggccgcgagg gtggccatcc ggtcccgcgt
     3001 ttcgacggag gcgctaccga tgtgcggggc gaggaccacg ttctccagct ccagcagctt
     3061 cgggtgcacc tcgggctcgt tctcatacac gtcgaggccg gcggcgaaga tgcgccggtc
     3121 ggcgagggcc tcggcgaggg ccgcctcgtc gacgacgggg ccacgcgagg tgttcacgag
     3181 gacggcctcc ggcttcatgc cgtcgagctc gtcggcgccg ataaggtgtg tggtctcggg
     3241 cgtaaggggg gtgtggatgg agacgaagtc ggccgcctcg agcagatcgc ccagttgcat
     3301 gtatcgggct tctatctcac gctccgcctc ctctttgcgg gagcggttgt ggtagaggat
     3361 ctccatcccg aagcccctgg cacggcgggc tacggcctgc ccgattcggc cgaacccgac
     3421 gataccgagc ttctttcccc acacgtcgcg gcccatgagt tgtttgggcc cccaccactc
     3481 ccaccctcca gcccgcagca ggcgctccgc ctctcccagg cgccgggcgg ctgccagcag
     3541 gagcatgaag gccacgtcgg ccgttgtctc gtccagcacg cctggggtgt tggttacgat
     3601 gatgcccctc tcaccggcgg cctctacgtc cacgttatcg tagccgacgg ccatgttggc
     3661 gatgaccctc aggccttccc cggcggcgtc catgacctca ccgtccatgg tctcggtagc
     3721 ggtcgagagg acgccggaga caccgcggac cgcctcgagc agctcgtcgc gctccggggg
     3781 gcgttcgtgc aggacggtgg tgtcgaacgg ttccaggacg cggagcccag cctccgggat
     3841 ctctcgggcg accagaacct tatctggcat cagccccaga cctcgtctag cctgccggtg
     3901 tagaagcgca gcgctttctc gtaggacctt atccccgggt ccgaggttgt ctccaccagg
     3961 tgctttagca gcaggtccac cgcctggcga tgctccccga cgttgtacag ggccagcgcc
     4021 aggaaggtct gcatggccct atcagctgga aactcggaga ccccctggcc cagaacctcc
     4081 accgcctcgg ggtactcgcc gagggatcgg tacgtgctgc ccaggccaag gagcgcgcca
     4141 cgcctatcct tttctggaag gtcgccggct acggccttca cgtagtgggg cacagcctcc
     4201 cgctcacggc cctggaggtc gtaaaggcac gacatctggt agttggcctc cgcgtcatcg
     4261 gggatctccg ccaggctgcg ggagatcaag gactccgctt cgcggtagtc accgacctct
     4321 cgcagcctga ccgcctcggc cagatcgaac cgtcgcaacg ctattcctcg tcgagcagtt
     4381 ggcgcagcac cgtctgaagg atgccgccgt tacgcaggta ctcgacctcg accggcgaat
     4441 cgacgcgcgc cttcgcctcg aactccgtca ccccgccgtc atcagcggtt gccttcacgg
     4501 tcagcacctt gcccggctcc aggtcggcaa ggccggagat gtcgaaggac tcgtggcccg
     4561 tcaggccaag agattcggcg ctctcaccgt ccgagtactg gaggggaaga acgcccatgc
     4621 cgatcaggtt cgagcggtgg atgcgctcga agctctccgc gatcacggcc ctgacgccga
     4681 gcaggaaaga accctttgcc gcccagtcgc gggaggatcc agaaccgtac tccttgccgg
     4741 cgagaacgag gagcggcacg ttctcctcag cgtactgcag gctcgcctcg tagatggtgg
     4801 tcttctcccc gtcggggaga tggacggtgt agccaccctc ctcgtcgacg agctggttgc
     4861 gcaggcggat gttgccgaag gtgccgcgca ccatgacctc gtggttgccg cggcgcgagc
     4921 cgtaggagtt gaagtccctc gagtcgacgc ccttctggat gaggtactg
//
LOCUS       DBNGVD010000260         3267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4066588, whole genome shotgun sequence.
ACCESSION   DBNGVD010000260 DBNGVD010000000
VERSION     DBNGVD010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4066588"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1197)
                     /gene="solA"
                     /locus_tag="AC16CM_07115"
     CDS             complement(52..1197)
                     /gene="solA"
                     /locus_tag="AC16CM_07115"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR3139635.1"
                     /translation="MRYDAIVAGLGGMGSATAYHLAGRGSRVLGLERYTPAHDQGSSH
                     GQSRIIRQAYFEDPSYVPLLLRAYELWEQLERESGEDLMRLTGGLMIGPPESHTFTGS
                     KATADEYGLPYEVFEAAELKRRYPAFEPTEGTVALFEEKAGFVRPEAAVKAHLARASA
                     MGADLRFGEPILTWRPTASGVRVETASGTYEAERLVVTAGAWAPKLLADLGLPLEVTR
                     QILFWLDPVGGVEPFLSDRFPIFIWEPEDGNSFYSIPAHDGPDGGVKVAFFRADGTPT
                     DPDSIDREVHDHELEFIRSYLQRYVPTLDGGLLYAKTCMYTNTPDEHFVISTHPEYPQ
                     VAIAAGFSGHGFKFCGVVGEILADLVTKGETDHPIGLFSPARLTTTG"
     gene            complement(1209..2801)
                     /locus_tag="AC16CM_07120"
     CDS             complement(1209..2801)
                     /locus_tag="AC16CM_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3139636.1"
                     /translation="MRTTDTGEGTASQTRTVASLIGGSTPEDAPGGRTNSVNPARTSE
                     TVAEVLLGDADTFLDACRAARAAQREWAGVPAPIRAQVIKKIGRIVENNKEALAHLVT
                     REIGKPYAESLGEVQEIVDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRNPVGVAAII
                     TAGNFPVAVPSWYMIPALTCGNAVVWKPAEYSPAIGDALAQLFIAGGLPDGVLNVVQA
                     EGPAAFDGLERALDEGLVDKVGFTGSSEVGKKIGELCGRHMQSPCLELGGKNPLVVMP
                     DADLDLAVEGALFSGFGTAGQRCTSLGTVIVHESVHDEFLRKLSEAVAAAKIGDPTED
                     VLYGPMLSERFHDRFIGFLELIEDHHSLHGSTGMGTITEDNPRKGFVGDPEAGIYCHP
                     TIVDGVTMEDEIYRTETFGPLVGVATFSDFEEAVAISNGHGYGLSAAIYTTDAKNALR
                     FRERNSAGMLSVNNSTSGAEAHLPFGGNGKSGNGSRLSGIWVIDQFTRWQSMNWDYAG
                     KLQKAQMDIADLPADLDFTLPD"
     gene            2920..>3267
                     /locus_tag="AC16CM_07125"
     CDS             2920..>3267
                     /locus_tag="AC16CM_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3139637.1"
                     /translation="MGVVDKAIANSVPIIPRPIVKKISSRYIAGDTLPEAISTIRDLN
                     KQGCVATIDLLGESTESKADAMSKLRDYEKVVDALDESDLNSGISVKLTGLGLTLNEE
                     LCRANLEEIVEYAA"
BASE COUNT          551 a         1048 c         1087 g          581 t
ORIGIN      
        1 cttccctgat agccggcacg tctaccggtc tctcatgccg gatctccatc gctagccggt
       61 ggtggtgagg cgggcaggag agaagaggcc tatagggtgg tcggtctcgc ccttcgtgac
      121 gaggtcagcc aggatctcgc ccaccacgcc gcagaacttg aagccgtgtc cagagaaccc
      181 ggccgctata gcgacctgcg ggtattcggg atgggtggag atcacgaagt gctcgtctgg
      241 ggtgttggtg tacatgcagg tcttggcgta cagcaacccg ccgtcgaggg tggggacgta
      301 cctctgcaag tagctcctga tgaactcgag ctcgtggtcg tggacctcgc ggtctatgct
      361 atctgggtcg gtgggcgtcc cgtcggcccg gaagaaggcg accttgactc cgccgtcggg
      421 cccgtcgtgg gccggtatcg agtagaagga gttgccgtcc tctggctccc agatgaagat
      481 cgggaagcgg tcggagagaa acggctcgac accccctacc ggatcgagcc agaacaggat
      541 ctggcgcgtc acctcgagcg gtagcccgag gtccgcgagc agcttcgggg cccatgcgcc
      601 ggccgtcacg accagccgct ccgcctcgta ggtgcccgag gccgtctcga cccgcacccc
      661 ggaggcagtc ggcctccacg tcaggattgg ctcgccgaac ctgagatccg cgcccatggc
      721 cgaggccctg gccaggtggg ccttgacggc ggcctcggga cggacaaacc cggctttctc
      781 ctcgaagagg gccacagtgc cctccgtagg ttcgaaggcc gggtagcggc gtttcagctc
      841 ggcggcttcg aagacctcgt agggcaggcc gtactcgtcg gcggtggctt tgctgcccgt
      901 gaacgtgtgg ctctcgggcg gcccgatcat cagccccccc gtgagccgca taaggtcctc
      961 gccggattcc ctctccaact gctcccacag ctcgtaggcc cgcaggagca aagggacgta
     1021 ggaggggtcc tcgaagtagg cctggcggat gatcctggac tgtccgtggc tggaaccctg
     1081 gtcgtgggcc ggggtgtacc gttcgaggcc gagcacgcgg ctgccgcgcc cggcgaggtg
     1141 gtatgccgtc gcgcttccca tccccccgag gccggctacg atagcgtcgt atctcacagg
     1201 cggggttcct agtccggtag ggtgaagtcg aggtcggccg ggagatcggc tatatccatc
     1261 tgggccttct gcagcttgcc cgcgtagtcc cagttcatgg actgccagcg ggtgaactgg
     1321 tcgatgaccc agataccgga gaggcggctg ccgttaccgc tcttgccgtt gcccccgaag
     1381 ggcaggtgcg cctcggcgcc gctggtcgag ttgttgacgg agagcatgcc ggcggagttt
     1441 cgctccctga aacgcagggc gttcttcgcg tcggtggtgt agatggcggc agagagcccg
     1501 tagccgtgcc cgttggagat ggcgacggcc tcctcgaagt ccgagaaggt tgcgacgcct
     1561 accagcgggc cgaaggtctc ggtgcggtag atctcatcct ccatcgtgac gccatccacg
     1621 atggtgggat gacagtagat gccggcctcg gggtcgccga cgaagccctt cctcgggttg
     1681 tcctccgtga tggttcccat cccggttgag ccgtggaggg agtggtggtc ctcgatcagc
     1741 tccagaaaac cgatgaaccg gtcgtggaag cgctcgctca gcatggggcc gtagagcacg
     1801 tcctcggtcg ggtcgccgat cttcgccgcc gcgacggcct cggaaagctt ccgtaggaac
     1861 tcgtcatgga cggactcgtg cacgatcacg gtcccgagcg aggtgcagcg ctggcccgcc
     1921 gttccgaagc ccgagaagag cgcgccctcg accgcgaggt ccaggtccgc gtctggcatc
     1981 acgaccaggg ggttcttgcc cccgagctcc aggcaaggag attgcatgtg cctgccgcag
     2041 agctcgccta tcttcttgcc gacctcggag gagcccgtga aaccgacctt gtccacgagg
     2101 ccctcgtcca gggcccgctc caggccgtcg aaggccgcag gtccctcggc ctgaaccacg
     2161 ttcagaacgc cgtcggggag gccgcccgct atgaaaagct gcgccagggc gtcgccgatg
     2221 gccggggagt actcggcagg cttccagacc acggcgttgc cgcaggtgag ggccgggatc
     2281 atgtaccacg agggcacggc aacggggaag ttgccggcgg tgatgatggc ggctacgccg
     2341 acgggattgc ggaaggtgaa gagctgcttg tctggcatct ccgaggggac cgtctggccg
     2401 tagaggcggc gaccctcgcc ggtgaagaag tcgcaggtgt caacgatctc ctgcacctcg
     2461 cccagagatt ccgcgtacgg cttgccgatc tcgcgcgtaa ccaggtgggc gagcgcctcc
     2521 ttgttgttct ccacaatgcg cccgatcttc tttatgacct gggcccttat gggagccgga
     2581 accccggccc actcccgttg cgccgctcgg gccgcccggc acgcgtcaag gaacgtatcg
     2641 gcgtccccga gcagcacctc ggccactgtc tccgacgtgc gtgccgggtt caccgagttc
     2701 gtgcgtccac ccggggcatc ttctggcgtg ctgcccccga tcagggaggc cacggtgcgg
     2761 gtctgcgaag ccgttccttc gccggtgtcg gtggtgcgca tcgtctacct ccagcgtctc
     2821 tcaactccca ttttcgtccc tatcgtacac caaagcctcg gatccctcct tcgagtagaa
     2881 tcaggggtaa taaaggcgtc ttttggagag attcgggata tgggagtagt ggacaaggca
     2941 attgcaaact cggtgccaat tattccgcgg cccatagtca agaagatctc cagccgctac
     3001 atcgccggcg acacgctccc agaggccatc tccacaataa gggacctcaa caagcagggc
     3061 tgcgtcgcca ccatcgatct cctcggggag agcaccgaga gcaaggccga cgccatgtca
     3121 aagctccgcg actacgagaa ggtcgtagac gccctcgacg aaagcgacct gaacagcggc
     3181 atctccgtca agctcaccgg cctcgggctg accctgaacg aggagctgtg ccgggcgaac
     3241 ctcgaggaga tcgtcgagta cgccgcc
//
LOCUS       DBNGVD010000261         3505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_969452, whole genome shotgun sequence.
ACCESSION   DBNGVD010000261 DBNGVD010000000
VERSION     DBNGVD010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_969452"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC16CM_07130"
     CDS             complement(<1..553)
                     /locus_tag="AC16CM_07130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3139638.1"
                     /translation="MDLGGGVAHLVLNPSAGKGRAGGLRKFVSEFLEDRGVRPVWHIT
                     EGPGHAGAIVGGLPEGAPVVAVGGDGTVHEVAETCVESGRVMGILPAGSGNDYVKALG
                     IGTRLRRALAVLVSGRVRVVNTAEVDGVPFNNGLGIGFDAEVAAGVALAPAYLGGTGR
                     YLWSVGRLLKDFRCHEARLTLDGR"
     gene            1030..1437
                     /locus_tag="AC16CM_07135"
     CDS             1030..1437
                     /locus_tag="AC16CM_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139639.1"
                     /translation="MSRDEAYSVVEGDEFGAIALEESQREVYRRRRVMYELENVPRVV
                     TARPEMILVVHDGAKVAYECRVFYKAPRPVGVVEQASVEATTERILELRSDADPVIRL
                     ISEKVEEFHAFRSSLLEDGEPAPERRVFYANEL"
     gene            complement(1796..2335)
                     /gene="thpR"
                     /locus_tag="AC16CM_07140"
     CDS             complement(1796..2335)
                     /gene="thpR"
                     /locus_tag="AC16CM_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3139640.1"
                     /translation="MARTFVAIFPPREVREALVHAARNLRNSRDFRLIGQEKIHLTLK
                     FLGDVAEQDLGTIEKALQPLGESHDPFEASTSTFGAFPSVQRARVLWAGIEQGSNQLR
                     TIAETVEDLLFPVGFERESRPYVPHLTLGRARARRAKLDLPQTSPPTLRFTVCGVDLV
                     KSTTDQNGVTYSKLASFRF"
     gene            complement(2340..3140)
                     /gene="larB"
                     /locus_tag="AC16CM_07145"
     CDS             complement(2340..3140)
                     /gene="larB"
                     /locus_tag="AC16CM_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR3139641.1"
                     /translation="MDHTENILAAVANGELSVATAAGRLKKGEVRYLDEFAALDLGRS
                     IRKGVPEVVYARGKSPAQTASICATLLESCKRVIVSSPTPEHEAEIRATLPDTPVRQA
                     GRSIVVGTGEPLPGGGRIGALSAGTSDLPVLEEALAVAREMGVSVRSFHDVGVAGLHR
                     LVAPLEELRGFDPDVLIVAAGMEGALPSVVSGLVPVPVIGLPTSTGYGLGGDGTAAIL
                     GMLQTCSPGLSVVNVDNGVGAGATAALIANRAAQGRKVQENGHKPIPD"
     gene            3214..>3505
                     /locus_tag="AC16CM_07150"
     CDS             3214..>3505
                     /locus_tag="AC16CM_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR3139642.1"
                     /translation="MSIIELIVALAAILGAAVLFTNAVEIMGERLNLGHGAVGSLLAA
                     VGTALPETMIPIVAILGAVIVGTGAAAAGEIGVGAILGAPFLLATLALFLVGL"
BASE COUNT          641 a         1089 c         1141 g          634 t
ORIGIN      
        1 cccggccatc tagcgtaagc ctggcctcgt ggcagcgaaa atccttgagc aacctcccga
       61 ccgaccacag ataccgtccc gtacccccca ggtaagctgg cgccagggcg acgccggcag
      121 cgacctcggc gtcaaagcct atgccgagcc cgttgttgaa gggaaccccg tctacctcgg
      181 cggtgttgac gacccttacc ctgccagaca cgagaacagc cagcgcccgc cgcagcctgg
      241 tgccaatacc tagggccttg acgtagtcgt tgccgctgcc ggccggcagg atgcccatga
      301 cccggcccga ctccacacac gtctccgcaa cctcgtgtac ggtcccatcc cccccaacgg
      361 ccacgacggg cgccccctcc ggtaaccccc ccacgatggc gccagcgtga ccgggaccct
      421 ccgtgatgtg ccagaccggc cgcacccccc gatcctccag aaactcggag acgaacttcc
      481 gcaacccccc ggcccgtcct ttgccggccg atgggttgag aaccagatgc gctactcctc
      541 cgcctagatc cacacccgga aaataccaga accgtgaccc accggcccgg tcaccgagta
      601 actcaagcta ttctcgaggt atagatcaga gcgtagtgaa ccgtttgcag gtgaggtcgc
      661 agttcatccc acagcatgac gaccgcgcga aaccttgcgg gagaggagca aggcaccgcg
      721 cggggtctcg tggaacgatg cggaatcggc tccgagtaga agtccggtaa gtggtgcgcc
      781 tgttctccgg tgtctcgaag acccgtcgga cataaggttg aagtaaaggt agagagcgat
      841 gtcgcgcccc gggtgagagt cgcgcagggt gatgaaaaga gtacaatacg ccccaacatg
      901 gccgtaccca tcgaaaattg cctgcacctc gaaggtctga aggttgctat cagatccgag
      961 taccgctgcc ctctatgtgg gaaggtcatg gaccttgcgg aagttgcggg gtgggttcgg
     1021 gaggctgaga tgagccgtga tgaggcttac tctgtcgttg agggtgacga gttcggagcg
     1081 atagctctgg aagaatcgca gagggaagtc tacaggcgac ggagggtgat gtacgagtta
     1141 gagaacgtac caagggtcgt gacggcgagg ccggagatga tcctggtcgt ccatgatggc
     1201 gcgaaggtgg cttacgagtg ccgcgtcttc tacaaagcac cgcggcccgt tggggttgtg
     1261 gagcaggcga gcgtcgaggc taccacagag agaattctgg aattgcgctc cgacgccgat
     1321 cccgtaatca ggctaatctc cgagaaggtc gaggagttcc acgcctttcg ttccagtctc
     1381 ctcgaggatg gggagcctgc gcccgagcgc agggtgttct acgcgaacga gctatgagcg
     1441 ctggcagcaa ccccggccag ccgctagaat caaccaccag atggatacca acttcaacag
     1501 cgcggtcgca tgagcggtag caccttcata gccctcgtcg tcatgatcct gatcctgctc
     1561 ttcatcgtca tgtcgatagg gcccctgatc tcggcgttct agcacgcacg gaccttcggg
     1621 tcctccgctt gtcagccaat ccagacctct gttcgtcggc acgctcgggc catcaccctg
     1681 gcctgtcgtc atgcgtcgcc tcgtcgtcgg ccggatccgg gagcggccgc acgttaccgt
     1741 agatagaggg cgctgtcctg ttctagcgcc tcgttgttcg tctgagcatg aagccctaga
     1801 agcggaagga tgcgagcttc gagtaggtca ccccgttctg gtctgtggtg ctctttacca
     1861 ggtctacgcc gcagaccgtg aagcggagag tgggcgggga ggtttgcggg aggtccagct
     1921 ttgcccggcg ggctcgcgcc cggcccaggg tcaggtgggg gacgtagggg cggctttccc
     1981 tttcgaagcc gacggggaac aggaggtctt ccaccgtttc ggcgatggtg cgcagctggt
     2041 tcgagccctg ctctatgccg gcccacagga cacgggctct ctgcaccgac gggaaagccc
     2101 cgaaggtcga ggtggaggct tcgaacgggt cgtggctctc accgagtggc tggagggctt
     2161 tctctatggt tcctaggtct tgctccgcta cgtcgcccag aaacttcagc gtaaggtgga
     2221 tcttctcctg gccaatgagg cggaaatccc tgctgttccg caggttccgg gccgcatgga
     2281 ccagcgcctc ccgcacttcc cgtggtggga agatggcgac gaaagtcctt gccacgggat
     2341 caatccggga ttggtttgtg gccgttctcc tgcaccttgc gtccctgggc agcacggttg
     2401 gcgatgaggg ccgccgtggc gcccgcgccg acgccgttgt ccacgttgac gacggagaga
     2461 cccggggagc aggtctggag catgccgaga atggcagcgg tgccgtcgcc gcccaggccg
     2521 tagccggtgg aagtggggag ccctataacc ggtacgggga cgaggccgga gacaacgcta
     2581 ggaagcgcac cttccatccc cgctgcgacg atcaggacgt cagggtcgaa ccccctgagc
     2641 tcttccagcg gagctaccag gcggtggagg ccggccacac cgacatcgtg gaagctcctc
     2701 acggaaacgc ccatctcgcg ggccacggcg agcgcctcct cgagcacggg cagatccgag
     2761 gtgcccgcgc tcagagcacc gatccggccg ccaccaggca gaggttctcc ggtaccgacc
     2821 acgatggagc gcccggcctg ccgcactggg gtgtcgggga gcgtcgcgcg aatctccgct
     2881 tcgtgctccg gggtagggga gcttacgatg acgcgcttgc acgactcgag cagggtggcg
     2941 cagatgctgg ccgtctgggc ggggctcttg ccgcgggcgt agacgacctc gggaacaccc
     3001 ttcctgatgg agcgtccgag atccagggcc gcgaactcgt ccaggtagcg aacctcgccc
     3061 ttcttcaacc gcccggcggc agtggcgacc gagagctcgc cgttcgcgac ggcggccagt
     3121 atattctcag tatgatccat ttcctccgaa tctgggtata gtacgggcca tgaatctaac
     3181 acgtcgtttc ctcgaaagtt gggagtgggg tcaatgtcga taatcgagct catagtggca
     3241 ttagccgcga tactcggcgc ggcggtgctc ttcacgaacg ctgtcgagat catgggcgag
     3301 cgactgaatt tgggccacgg ggcggtaggg agcctgctgg cagcggtggg gacggcgctt
     3361 ccggaaacga tgatccccat cgtggccata ctcggcgcgg tcatcgtggg cacgggagcg
     3421 gccgcagccg gcgagatagg cgtcggtgcg attctgggcg ccccgttctt gctggccaca
     3481 ctggctttgt tcctggtggg gctct
//
LOCUS       DBNGVD010000262         5948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2062175, whole genome shotgun sequence.
ACCESSION   DBNGVD010000262 DBNGVD010000000
VERSION     DBNGVD010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2062175"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC16CM_07155"
     CDS             <1..478
                     /locus_tag="AC16CM_07155"
                     /inference="COORDINATES: protein motif:HMM:NF049060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7144 family membrane protein"
                     /protein_id="HIR3139643.1"
                     /translation="RWLETERRIAMAQHSEPMPGRTSDPMSPAGGAVTHRPGWFTFTA
                     VMMFTVAGFELLSAILAFGGTGWWVTEMGNLVYTNFFFWGVLDLIVALIALYAGVSLL
                     RGGEFGLTMGYLFAGLTAIRWLFVIPAAPILSVVVIALCVMVIYGLAKHSDYAEGV"
     gene            603..1097
                     /locus_tag="AC16CM_07160"
     CDS             603..1097
                     /locus_tag="AC16CM_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR3139644.1"
                     /translation="MATLSVLKFNDPYGADRVLVALQGMQERELITLEDAAVVSWPEG
                     NKKPKTRQMHGTAGAGATWGAFWGFLFGLIFFVPFLGAAIGAGMGGMTGALADVGIDD
                     DFIKQVREKVTEGTSALFALTSGATAPDKVVDELRQYDFEIISTNLPEEQERKLRDAF
                     AQTS"
     gene            1226..1879
                     /locus_tag="AC16CM_07165"
     CDS             1226..1879
                     /locus_tag="AC16CM_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HIR3139645.1"
                     /translation="MSDLLTGLLFLAVGGSVAPPLLFLTILFLGSRRPLPNAGALVLG
                     YLTVCAAVGISGLVLFGDAGSAISVVGRVISVTLGALLLVLGLGSLLGSSGSDTSPPG
                     WLASIDNSSPFRAFAVGMALFPLQVKNLAIFIVCLNLIIASDLGTEGSILAITPVLLI
                     FALPVLALMGLYAAVPQRASSMLGSLQVWMGKHGRTITVVICLVFGAFFLLRGLLGA"
     gene            1988..3127
                     /locus_tag="AC16CM_07170"
     CDS             1988..3127
                     /locus_tag="AC16CM_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3139646.1"
                     /translation="MKEASGNQADDKSGIGESITSALRRPSFFRVALLLAALVVVFTG
                     MRLAAPILNPILFALVLALLFSPIYAWLRRRRIPTPLALVIMLVGLTVLFATIAGIMG
                     VSIARFSGDIGSYASKLNGQLDSIQSLTSNLGVSRDDLQKALDPRAVTGAIGSILGGV
                     ADFLSNLFLILIIVLFLLAEGPALMSRLRASTGENHPQVARLTMFGRSVIRQLGLRAI
                     VNLVTGTGVTILLLVLRVDFPLMWGILAFFLSFIPWIGLPLAVAPAVVLALAEHGLTS
                     ALLVIAGVIGINVLAENALSPMLMGRGLSISPTILFIGFIFWAWLLGGPGAFLAAPLT
                     IFLILMLGSFPETQWLANVMGVGEPEATDEEEPAVAPTQRPSVSP"
     gene            3141..4061
                     /locus_tag="AC16CM_07175"
     CDS             3141..4061
                     /locus_tag="AC16CM_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3139647.1"
                     /translation="MRGRERIGIIELGRRSILSFFFEHPMATYSAALAYRGLFGLFPF
                     LLILVLLVGAIGLPDLFEQTAEDAQQYRQVPGQLEPVVEPAREQLQPLLGMLERAEER
                     AGSKLLIFGVGIALWSVSAVARTLTEAFNVAYNVDETRRWYTQLAASLAFGPILAMAV
                     IVSVALLLIGPRLVHSVAELVDLDEMFVLLWGWLRFPVALLLLGAVLTVVYRFGPNIR
                     QRFRSILLGAALAVTLWVISSVLFAFYLSNFATFGFAYGSIGAAVGLLLYLYLCASVV
                     LLGAELNAAIYHSVGDEAGGKQNQGGHDRE"
     gene            4054..4605
                     /locus_tag="AC16CM_07180"
     CDS             4054..4605
                     /locus_tag="AC16CM_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR3139648.1"
                     /translation="MSEEPRYRQRLSSLATNWRALALRGLIALLFGLVILFWPGLVLT
                     VLSILFGIYAAVDGAITFVPALRSPERGPQRTLPLTEGVVGIVAGLVALLWPGLTAVG
                     LVYVIAGWAVATGVLKVLTAMLLRTEARMLAGSGVLSVLFGILLVVLARSDVPFLAPF
                     IGGFSLVVGLALIVFAFRLRERG"
     gene            4788..5075
                     /locus_tag="AC16CM_07185"
     CDS             4788..5075
                     /locus_tag="AC16CM_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139649.1"
                     /translation="MGCIFALFGGLFPRLALLVLWIARPALVGAAFGTFLLPLLGLIF
                     LPFATLIYVIVYTPGVGLVGFDWFWVILAVLLDVGHWTTSYTQRNQIPGVR"
     gene            5111..5644
                     /locus_tag="AC16CM_07190"
     CDS             5111..5644
                     /locus_tag="AC16CM_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR3139650.1"
                     /translation="MMQKIGPVQLLAIGFPPDADFEGRIMAEIERLEAHDTIRVLDLL
                     FVTRDAETGDLLALDYQGEDLGAIVGALLGFEFEDEAMRPNGSAEGTEGHAFGLSQDE
                     IQDIAASLDPGSSAGFVLIEHVWARDLKEAFRDAGGFPLGEGFLTPEGLAEIAIEVVA
                     MSEAIEELEADQANAGA"
     gene            5677..>5948
                     /locus_tag="AC16CM_07195"
     CDS             5677..>5948
                     /locus_tag="AC16CM_07195"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3139651.1"
                     /translation="MGLVANRAAGRMQARQTYRTMARMERRRSFVQNQFGMRQDFGRQ
                     EEPVEAAAPAQPSEPGYVGELERLSQLRDQGIITNEDFEAKKKQILG"
BASE COUNT          953 a         1873 c         1926 g         1196 t
ORIGIN      
        1 gagatggcta gagaccgaga ggagaatagc gatggcgcag cacagcgaac cgatgccggg
       61 aagaacgagt gaccccatga gccctgcggg cggcgcggtg acgcacaggc ccgggtggtt
      121 caccttcacg gccgtcatga tgtttacggt tgcaggcttc gagctcttga gtgcgatcct
      181 ggcgttcggg gggaccggct ggtgggtcac cgagatgggc aacctggtgt acaccaactt
      241 cttcttctgg ggcgtgctcg acctgatcgt cgcgctcatc gctctctacg cgggggttag
      301 tctcttgcgc ggaggagagt ttggcctgac gatgggctac ctcttcgccg gcctcaccgc
      361 catcaggtgg ctcttcgtca tcccggcggc cccgatactc tccgtggtcg tgatcgcgct
      421 ttgcgtgatg gtcatctacg ggctggccaa gcactccgac tacgccgagg gcgtgtgagg
      481 gcatcagggg cacgacctga cggatcactg ccaggatgag gacccgtggg gggaggtcgc
      541 ctcctcgagc tctgctcggt gcgcatggca tccgtagact cggatagaga ggagaaacgc
      601 tagtggcgac actatcggta ctcaagttca acgaccctta cggcgctgac agggttctcg
      661 tggcgctcca gggcatgcag gaacgggagc tgatcacgct ggaggacgcg gcggtggtca
      721 gttggccaga gggcaacaag aagcccaaga cccgccagat gcacggtacc gccggcgccg
      781 gcgccacctg gggtgccttc tggggcttcc tgttcggcct gatcttcttc gtaccgttcc
      841 tcggtgcggc gataggcgca ggcatgggcg gtatgacggg cgcgctggcc gacgtcggca
      901 tcgacgacga cttcataaag caggtccgag aaaaggtcac ggagggcacc tcggcgctct
      961 tcgccctcac cagcggggct acggcacccg acaaggtcgt agacgagctc aggcagtacg
     1021 acttcgagat catctcgacc aaccttcctg aagagcaaga gcggaaactc cgcgacgcat
     1081 tcgcgcaaac aagttaagcg tgacgatcct gcggggcggt ccattgccgg gccgccccgc
     1141 ggcaacgaga gagagccgtt ttgtcacaga gatcccaggt ctgtggtcgc ccggactcag
     1201 gtgtcaaccg gctgacggcg tgcctgtgag cgaccttctc accgggctcc tatttctggc
     1261 cgtcggtggc tcggtcgcgc ctcccctgct cttcctcaca atcctgtttc tgggttccag
     1321 acgtcccctt cccaacgccg gtgcgctggt gctcgggtac ctgacggtgt gtgcggcggt
     1381 gggcatctcg gggctcgttc tcttcgggga cgcggggagc gctatctctg tggtgggccg
     1441 ggtcatcagc gtgaccctcg gggccctgct cctcgtactc ggcctcggga gcctccttgg
     1501 ctcttccggt tctgacacct cgccgccggg atggctggca tccatagaca acagttcgcc
     1561 cttcagggct ttcgccgtgg gtatggcgct cttcccgctg caggtcaaga acctcgccat
     1621 cttcatcgtg tgcttgaacc tgatcatcgc gtcggatctc ggcaccgaag ggagtatcct
     1681 ggcgatcacg ccggtgctcc tgatcttcgc cctcccggtc ctggcgctca tgggcctcta
     1741 cgccgctgtc cctcagcggg cctcgtctat gctcggctcc ttgcaggtgt ggatgggcaa
     1801 gcacggccgc acgatcacgg tggtgatctg cctggtgttc ggggcgttct ttctcctcag
     1861 gggtcttctc ggagcgtgag accgcgagga gcaggtgatt gccgctggga cagccgcatg
     1921 agcagggcga gagaccgtgc aaaacacgcc gggcgacggg gcctggcgca aacggaaagg
     1981 tcagatcatg aaagaagctt cgggcaacca ggcagacgac aagtccggca tcggtgagag
     2041 cataacctcc gcgctgcgca ggccatcgtt cttccgggtg gcgctgttgc tcgccgcgtt
     2101 ggtggttgtg tttaccggga tgcggctggc agcgcctatc ctgaatccta tcctcttcgc
     2161 gctcgtcctg gcactcctgt tcagcccgat ctacgcctgg ctcaggcgac gccggatccc
     2221 taccccgcta gccctggtca tcatgctcgt agggctaacc gttctattcg ctaccatagc
     2281 aggcatcatg ggtgtctcga tcgccaggtt cagcggtgat atcgggagtt acgccagcaa
     2341 gctgaacggc cagttggaca gcatccagag cctgaccagc aacctcgggg tctcgcgaga
     2401 cgacctgcaa aaggcgttgg atcccagggc cgtcacaggg gccatcggct ccatcctggg
     2461 cggcgtcgcc gactttctga gcaacctgtt cttgatcctc atcatcgtgc tgttcctgct
     2521 tgcggaaggt ccggccttga tgagccgcct gcgcgccagc acgggtgaga atcatcctca
     2581 ggtagcccgg ttgaccatgt tcggccggag cgtgatacgc cagcttgggc ttcgcgccat
     2641 agtcaacctc gttacgggca ctggtgtaac catcctgttg ctggtgttga gggtcgactt
     2701 tccgctgatg tggggcattc tcgccttctt ccttagcttc ataccctgga tcgggctgcc
     2761 cctcgcggtt gccccggccg tggtactcgc cctggccgag cacggcctca ccagcgcgct
     2821 gctggtcata gcgggggtaa ttggcatcaa cgtgctggcc gagaacgcgc tctcgccgat
     2881 gctcatgggg aggggcctga gcatctcgcc gacgatcctc tttatcgggt tcatcttctg
     2941 ggcgtggttg ctgggaggtc cgggagcatt tctggcggcc ccgctcacga tcttcctgat
     3001 cctgatgctc ggaagcttcc cggagacgca gtggctcgcc aatgttatgg gcgtgggtga
     3061 gcccgaggcc acagatgaag aggagccggc ggtagcgccg acccaacgcc cgagcgtgag
     3121 cccctagcag gacagacggt gtgcgcggcc gggagcgaat agggatcatc gagctcggcc
     3181 gacgctcgat cctgagtttc tttttcgagc accccatggc tacgtactcg gccgcgctcg
     3241 cctaccgcgg gcttttcggg ctcttcccgt tcctgctgat actcgtcttg ctggttggcg
     3301 cgatcggctt gcccgacctg ttcgagcaaa cggcggaaga cgcgcagcag taccggcagg
     3361 ttcccgggca actcgagcct gtggtcgagc cggccaggga gcagctccaa ccgctcctgg
     3421 ggatgctcga gcgggccgag gagcgggccg ggagcaagct gctcatcttc ggggtaggca
     3481 tcgcgttgtg gtcggtctcg gcggtggccc gcactctgac cgaggccttc aacgtcgcct
     3541 acaacgtcga cgagacccgc cgctggtaca cacaactggc cgcctccctg gctttcgggc
     3601 ccatcctcgc tatggctgtc atcgtctcgg tcgcgttgct cctgattgga ccgcggttgg
     3661 tgcatagcgt ggccgagcta gtggacctcg acgagatgtt cgtgctcctg tggggttggc
     3721 tacgctttcc cgtggccctt ttgctgctcg gcgccgtgct caccgtggtc taccgtttcg
     3781 gccccaacat caggcaacgt ttccggtcta ttctcctcgg cgcggccctt gccgtaacgc
     3841 tgtgggtgat ctcctcggtc ctgttcgcct tctacctgtc aaacttcgcc accttcggct
     3901 tcgcgtacgg gagcataggt gccgctgttg gtctactcct gtacctctac ctctgcgctt
     3961 cggtggtgtt gcttggcgcg gagctgaacg cggcgatcta ccattctgtc ggggacgaag
     4021 ccggcggcaa gcaaaaccaa ggaggacacg atcgtgagtg aagaaccacg ttaccggcag
     4081 cgactaagct ccctggcaac caactggcga gccctcgctc tgcggggcct catcgccctc
     4141 ctgttcggcc tcgtgatcct gttctggccc ggcctcgtgc ttaccgtcct gtccattctg
     4201 ttcggcatct acgcggcggt cgacggcgcg atcaccttcg tcccggccct cagatccccg
     4261 gagagaggcc cgcagaggac acttcccctg acagaaggcg tggtcggcat cgtcgccggg
     4321 ctcgtggccc tgctgtggcc cgggctcacg gcggtcgggt tggtctacgt catcgcgggg
     4381 tgggccgtcg cgacaggcgt gctcaaagtg ctcacggcga tgctcctgcg caccgaggcc
     4441 aggatgctgg ccggaagcgg cgtcctgtcg gtgctcttcg gcattctcct ggtggtcctg
     4501 gccaggtccg acgtaccgtt cctcgcgccc tttatcggtg gcttctccct ggtcgtcggc
     4561 ctcgccctga tcgtcttcgc cttccgcctg cgcgaacgcg ggtagcccat gatctctcac
     4621 gtgaggaccg atacctacgc gatcctcagc agcgggatat cccctagggg atatgcgtct
     4681 ctgggggatg aacgcccacc tcgccggggc ttatgctccg gcaagcgtca tgcaagacag
     4741 acgcgcgaac cacggaggac cggaccaacc gacgggaggt aacgaggatg ggttgtatat
     4801 tcgcgctatt cggtggcctc ttcccgcgcc tcgcgctgct cgtcctttgg atcgccaggc
     4861 ccgccctcgt cggggcggcc ttcggcacct ttctcctccc cttgctcggc ctcatcttct
     4921 tgcccttcgc gaccctgatc tacgtgatcg tctacacgcc tggcgtcggc ctggtcggct
     4981 tcgactggtt ctgggtcatc ctcgccgtcc tgctcgacgt tggccactgg acgaccagct
     5041 acacccagag aaaccagatc cccggggtac gatgaaacca catgagcaga ccatctagcg
     5101 gaggtgcgcg atgatgcaga agattggacc cgttcaactc ctggcaattg gcttcccacc
     5161 ggacgccgat ttcgagggca ggatcatggc cgagatcgag aggcttgaag cacacgacac
     5221 cattagggtc cttgacctcc ttttcgtgac cagggacgcc gagacggggg atcttctggc
     5281 cctcgactac cagggtgagg acctgggcgc gatcgtgggt gcgttgcttg gattcgagtt
     5341 cgaggacgaa gccatgcggc ccaacgggtc ggccgagggt acggagggcc acgccttcgg
     5401 gctctcgcag gacgagatcc aggacatagc cgcctcgctc gatccgggaa gctccgcagg
     5461 cttcgtcctt atcgagcacg tgtgggcgcg ggatctgaag gaggcttttc gtgacgctgg
     5521 gggcttcccg ctcggggaag gctttctgac gcccgaaggc ctggccgaga tcgctatcga
     5581 ggtcgtcgcg atgtcagagg ccatagagga gctggaggcc gatcaagcca acgccggggc
     5641 gtagtaacgt tcggccagag agaagggaga gacgagatgg ggctggtagc caaccgggcc
     5701 gcgggccgga tgcaggcgcg gcagacctac agaacgatgg cgcgcatgga gaggcgccgc
     5761 tcgttcgtac agaatcaatt tggaatgcga caggacttcg gacgccagga agagccggtc
     5821 gaggcggccg ccccggcgca accctccgag ccggggtacg tgggtgagct cgaacggcta
     5881 tcccagctca gggaccaggg catcatcacc aacgaggact tcgaggccaa gaagaaacag
     5941 atcctcgg
//
LOCUS       DBNGVD010000263         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_58391, whole genome shotgun sequence.
ACCESSION   DBNGVD010000263 DBNGVD010000000
VERSION     DBNGVD010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_58391"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1424)
                     /locus_tag="AC16CM_07200"
     CDS             complement(33..1424)
                     /locus_tag="AC16CM_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3139652.1"
                     /translation="MEAGKRLETTVGTILAGRYKVESTLGSGGMAVVYRAEDDILGRA
                     VALKTLHEEYAEVPSFQRRFRQEARAMASLDHQNIVKVYDISQDGEVPFIVVECVAGR
                     DVGDLLANRRGGRLNEQFAKRMAEQLLLALSYAHRRGIIHRDIKPSNILLTQEGIVKV
                     ADFGIARIIEEDDAGGAPGEIVGSARYMSPEQLRGEDATPRSDIYSVGVLLYHCLTGR
                     PPFSGDVRSLARQHIHKDPTPPRKLNRRISPATEAVIMKALQKDPRERYFSASAMLDD
                     IEIEAPSKKTTETPKLARRKTRSAGLLLATTLAVMLILGGGVALASSLIGFPQDGGVA
                     GTLSRMNPVETNPPPPPKNTGETAAQEDAPDQTGSKNVASVTEDEPSGKQDQPEKVPV
                     PDVTAYYDYYAADALQSSGFKVDYVRDYRDGYAPRGVTWATDPVAGSMAPEGSTVTVY
                     ATPKDLPLPPRLR"
     gene            1603..>1877
                     /locus_tag="AC16CM_07205"
     CDS             1603..>1877
                     /locus_tag="AC16CM_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3139653.1"
                     /translation="MKTEFDAYLRYDELTRTLRALAEEYPDLCKVRSIGKSYGGRDIW
                     LAELTNSATGPAGTKPAFWVDGNTHAGEVTGSMAALYLIEHLLENHGS"
BASE COUNT          302 a          617 c          604 g          354 t
ORIGIN      
        1 tgctgacaag cgcaggcgtt agccggagcg tgctagcgaa gcctcggggg aagggggagg
       61 tctttgggcg tggcgtagac ggtcacggta gagccttcag gggccatgct tccggcgacg
      121 gggtccgttg cccaggtaac gccgcgtggg gcgtagccat cgcggtagtc gcggacgtaa
      181 tcgaccttga agccgctgct ctgcagggcg tcggcggcgt agtagtcgta gtaggccgtc
      241 acgtcgggca cggggacctt ctcgggctgg tcctgtttac cggatggctc gtcctccgtg
      301 acggaggcca cgttcttgct gccggtctga tccggggcgt cttcctgggc cgccgtctcg
      361 ccggtgttct tcggcggggg cggggggttc gtctcgacgg ggttcatgcg gctcagggtc
      421 ccggccaccc cgccgtcctg ggggaacccg atcaggctgg aggcgagcgc caccccgccg
      481 ccgaggatta gcatcaccgc cagcgtggtg gccagaagga ggcccgcact gcgtgtcttg
      541 cggcgcgcca gtttgggggt ctccgtcgtc ttcttgctcg gggcctctat ctcgatgtcg
      601 tccagcatgg cgctcgccga gaagtacctc tccctcgggt ccttctggag tgccttcatg
      661 atcaccgcct ccgtggcggg cgagatccgg cggttcagct tgcgcggcgg cgtagggtcc
      721 ttgtgaatgt gctgcctggc gaggctcctg acgtcgcccg agaacggcgg ccttcctgtc
      781 aggcagtggt agagaagcac gcccacggag tagatgtcgc tccgcggcgt cgcgtcctct
      841 cccctgagct gctccgggga catgtagcgg gcgctgccca cgatctcacc gggcgcgccg
      901 cccgcgtcgt cttcctcgat gatgcgggcg ataccgaagt cagcaacctt gacgatgccc
      961 tcttgcgtga ggagtatgtt tgagggcttg atgtccctgt ggatgatgcc ccgcctgtgg
     1021 gcgtaggaga gcgcgagcaa gagttgctcg gccatcctct tcgcgaactg ctcgttcaga
     1081 cggccaccac gccggttggc cagcaggtcc cccacgtccc ggccggccac acactcgacc
     1141 actatgaagg gcacctcgcc gtcctgggag atgtcgtaga ccttcacgat gttctgatgg
     1201 tccaacgagg ccatagcgcg ggcctcctgc ctgaagcgcc gttggaaaga gggcacctcg
     1261 gcgtactcct cgtgcaaggt cttgagcgcc acggcacggc cgaggatgtc gtcctccgcc
     1321 ctgtagacga cagccatccc tccggaaccg agcgtactct ctaccttgta acgccccgca
     1381 aggattgttc cgaccgtggt ttccagtctc tttcccgcct ccaactaaaa ctccccggaa
     1441 tgcatcaatt ataactgccc ccgcacactc caggtacact aaccgagggt tagcacgacg
     1501 ttacccaata tgctggagcg tgtcaatcgc gagggccaca tctagtggcc cttccgcgcc
     1561 ataatggtac gtgatccacc ccggacccgc cgaaaggaga tcatgaagac cgagttcgac
     1621 gcctacctgc gctacgacga gctcacccgg accctgcgcg cgctcgctga agagtacccc
     1681 gacctctgca aggtacgctc catcggcaag agctacgggg gacgagacat ctggctggcc
     1741 gagctcacca actcggcgac gggtccggct ggcacgaaac cagccttctg ggtggacggc
     1801 aacacccacg ccggtgaggt taccggctcg atggccgcgc tctacctgat cgaacacctc
     1861 ctcgagaacc acggctc
//
LOCUS       DBNGVD010000264         6413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1335145, whole genome shotgun sequence.
ACCESSION   DBNGVD010000264 DBNGVD010000000
VERSION     DBNGVD010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1335145"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16CM_07210"
     CDS             complement(<1..851)
                     /locus_tag="AC16CM_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3139654.1"
                     /translation="MSEDVRPGGTYKTLAEQREVLTPAEERFERLRQTIGLVLGPVAF
                     LIMYFLPLPLPRDQHTLAAILVFTIIYWLSEAIPIPVTAVLALALCVLFNVPSVGPNA
                     EDAPGDIVFAGFADPVIFLFIGAFILAQAMITHGLDRRFAFLVLSLPGVGRSTYGVII
                     AFGVIAASISAFISNTTTAAMLLPIGLGMMAALGGMVSDQSDTHRDVSRLRFGTALML
                     MISYGAGVGGLLTPIGTPPNLIGIGFIEEETGVRISFFDWVLTAFPIVVLMFIALCTI
                     LILLNRP"
     gene            complement(1039..1293)
                     /locus_tag="AC16CM_07215"
     CDS             complement(1039..1293)
                     /locus_tag="AC16CM_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139655.1"
                     /translation="MSTEKDKGLIGSTSDQLKETTGILKGGGPWHSANSDVYHNNPSC
                     QTGGSIRPENVVQGTGDKPLCGECEKLNSAGGPLGNLTGL"
     gene            complement(1716..2810)
                     /locus_tag="AC16CM_07220"
     CDS             complement(1716..2810)
                     /locus_tag="AC16CM_07220"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR3139656.1"
                     /translation="MESGFGATAPYTVGVEEEFQLVDPRSRELTPAVEGVLAAGNGTK
                     LVTSELSQSCVEMLSPVFGSVGELARELPGLRREVRDLARSRGVEIVGAGAHPFSEPT
                     EQPLTQGAHNLRVEEEMGWVARTQAIYGLHVHVAVPDGEAAIRAMNTLAGYVPLIVAL
                     SNNSPFWRGSDTRLGSTRIKVFEMFPRSGLPPAFRSWEDFEDHIGALVAAGAIPDYSW
                     CWWDARPHEKFGTVELRSPDVQTDESYTAALVALVQCLVVRAASGDVPITDPVYNAEN
                     KWRAVRYGMDADFYDFAANRGVQARSMVRTLVEELRPVSQDLACEEQLLGVLEIADRG
                     TGAELQRAALERSGSLEGVVDYLIENTAPG"
     gene            3003..3719
                     /locus_tag="AC16CM_07225"
     CDS             3003..3719
                     /locus_tag="AC16CM_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3139657.1"
                     /translation="MSGEGFGKKVAVVTAAGSGMGAACARELSRRGYRVVLMSPSGRA
                     EDLADELGGLGLRGSVTEVGDLESLVGEALDRYGRIDGVVNSTGHPPSGEILDLTDEE
                     WYEALDLVLLNVVRTARLVTPTMASQGGGAIVNISTYSAFEPAPEFPLSSSLRAALAS
                     FTKLYSNRYGAEGIRMNNILPGYMENFDIDEQTLSSIPMQRQGSLAEIAGTAAFLLSD
                     AAGYITGQNIRVDGGITRSV"
     gene            4172..4744
                     /locus_tag="AC16CM_07230"
     CDS             4172..4744
                     /locus_tag="AC16CM_07230"
                     /EC_number="1.13.11.20"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0017172 - cysteine dioxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HIR3139658.1"
                     /translation="MITESSATAELVGGLEALATLAPERAVRKAVPFLTRLTDGAAYL
                     AEHVAPLFREAKDADEWYVARRWEGEGDSCSLEIFVWPAGSGTLIHDHTSWGVFRCVV
                     GTTLEKRYERLDDGSRFEHARLKKAWQHAWGPEDGISTVLPGNGGIHRVENPGVIPAI
                     SVHLYGPRLGEMDGRDYDPSREYVCDRRPA"
     gene            5059..5934
                     /locus_tag="AC16CM_07235"
     CDS             5059..5934
                     /locus_tag="AC16CM_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR3139659.1"
                     /translation="MMASERKTIVVTGATGLQGGAVARRLLLDGWHVRGLTRNAASKR
                     ARALEELGAEVAQGDMAEAASLRPVFEGAYGVYSVQNPFIGGPEQEVRQGKNVAEVAK
                     DAGVEHLVYGSAGTGRKGTGVPSWETKLQIEDHIKALELPLTILRPTAFMELLTHKKF
                     FPPVAAWQVMPKLMGSSRRLPWLCTEDLGVIAARAFAEPRSFVGRDLTLASDVNSLEE
                     CRSEYREVMGTNPRRLPMPAWLFHRFGFVGKDLTTMWRWLRTGDVDLDTSRTRAIHPD
                     ALTVGGWLRKQKAAE"
BASE COUNT         1228 a         1981 c         2034 g         1170 t
ORIGIN      
        1 tccgggcggt tgagcaagat caggatggtg cacagcgcga tgaacatgag tacgactatc
       61 gggaaggctg tcaggaccca gtcaaagaag ctgatcctca cgccggtttc ctcctcgatg
      121 aagccgatgc cgatgaggtt gggcggggtc cctatgggtg taagcaaacc cccgacgcca
      181 gcaccgtaag agatcatgag cataagtgct gtcccgaagc gtagccggct cacgtccctg
      241 tgggtatctg actgatcgct caccatccca ccgagggcag ccatcatccc gagtcctatg
      301 ggcagaagca tggcggcggt cgtggtgttg gagatgaaag cgcttatcga ggcggcgatg
      361 acgccgaagg cgattatcac gccgtaggtc gagcgcccta cgcccggcag cgagagcacg
      421 aggaaggcga atctgcgatc gaggccgtgg gtgatcatgg cttgcgcgag gatgaatgcc
      481 ccgatgaaca ggaatatgac ggggtcggcg aaaccagcga agacgatgtc gccaggagcg
      541 tcttcagcgt tcggaccgac gctcggtacg ttgaaaagca cacatagagc cagggccagg
      601 accgccgtga ccggaatggg gatagcctcg gagagccagt agatgatggt gaagacgagt
      661 atggcggcga gcgtgtgctg atccctcggt agcggcagcg gaaggaagta catgatcagg
      721 aacgccaccg gcccgagcac gagccctatg gtctgacgca gccgctcgaa cctctcctcc
      781 gccggtgtaa gcacctcacg ctgctctgca agcgtcttgt aggtgcctcc cggcctaacg
      841 tcttcgctca agaacccctc ccctcgagaa aaccacagct atcaatgcct accctatcta
      901 ccaatcatct caaaaccccg aaagctaggc ccttcggccg aagcgggccg actagtgagg
      961 acgttagccc gagcgtgctt acaagccgac gctagccgct gtgctgacaa gcgaaggccc
     1021 gcagggccga agcgtcctct acaggccggt gaggtttcct agggggccgc cagcgctgtt
     1081 gagcttctcg cattctccgc acagcggctt gtctccggtc ccctgcacca cgttctcggg
     1141 cctgatgcta ccgccggtct ggcagctggg gttgttgtgg tagacgtccg agttggcaga
     1201 atgccatgga cccccgccct tcagaatgcc ggttgtctcc tttagctggt ccgaggtaga
     1261 gccgatcagg cccttgtcct tttccgtgga cacgcgctcc tccttacgta gttcccaacc
     1321 ttggcttatt taagcagatc gcatatgcga atacactccg ccgcaccccg acgagtgccg
     1381 cttgatcgtc ccgcatttcg tggcgtttgt ttcgcggaaa ccacgtcggg taaatagacg
     1441 accaacgttc gtagagagcg agacaggagg tgagcgaacg tgggactcgg caaccttggt
     1501 gacagcctgg gtgatctcag caacctgaac ctggaccagc tacaacagta cctgccgaac
     1561 acggattttc ccgccgatgt ccctcgtact ttttgggacg cccctggcga catctaccgg
     1621 gctgaacagg tccgtccttc agagaacggt aacctgagct tggaaaaggc atcaggcatt
     1681 cttctgaagc ctatatcctc cttcgaaaac ctacctcagc ccggggcagt gttctcgatc
     1741 aggtagtcga ctacgccttc cagcgaaccg ctcctctcca gggcggcacg ctggagttcg
     1801 gcccccgtac cgcgatctgc gatctctagc acgccgagca gttgctcttc gcaggccagg
     1861 tcctgggaga cgggccgcaa ctcctcgacg agggtgcgga ccatggagcg ggcctgcacc
     1921 cccctgttgg cggcgaagtc gtagaagtcg gcgtccatcc cgtagcgcac ggcgcgccac
     1981 ttgttctccg cgttgtacac ggggtccgta atgggcacat ctcccgaggc ggccctgacc
     2041 accaggcact gtacgagggc caccagggca gccgtgtagg actcgtcggt ctgcacgtcc
     2101 ggcgagcgga gctccacggt gccaaacttc tcgtggggcc tggcgtccca ccagcaccag
     2161 gagtagtcgg ggatggcccc cgcggcgacc agggccccga tatggtcctc gaagtcctcc
     2221 caggagcgga acgccggcgg gaggccggaa cggggaaaca tctcgaatac cttgatacgc
     2281 gtggagccca gcctggtgtc ggagccgcgc cagaaaggcg agttgttgga cagcgcgacg
     2341 atgagcggca cataccctgc gagcgtgttc atggcccgga tcgccgcctc cccgtcgggg
     2401 acggcgacgt ggacgtgcag accgtagatc gcctgcgtcc tcgcgaccca gcccatctcc
     2461 tcctcgacgc ggaggttgtg ggccccttgc gtgagcggct gctccgtcgg ttccgagaaa
     2521 ggatgggccc ccgcaccaac gatctcgacg cccctgctgc gcgcgaggtc gcgcacctcc
     2581 ctgcgcagcc ccggtagctc gcgggccagc tcgcccaccg agccgaacac cggggagagc
     2641 atctccacgc acgattgcga gagctccgag gtgaccagct tcgtgccgtt cccggcggcg
     2701 agcacccctt cgacggccgg cgtcagctcg cgcgagcggg gatcgacgag ctggaactcc
     2761 tcctcgacac cgaccgtata gggcgccgtc gcgccgaacc cgctctccat ccggatactt
     2821 tagcagttga gcacgctccg gctagcacct ccgctcgtca gcacggcgct tcgcgcctcc
     2881 tcagcacgct cggctccgtc tcgctcgtcg gcaacgctta ggcttcgcct cgctttcagc
     2941 gttctgggct gtgttgtggg agggtatgat tcattggtga aggctataga gggagaaggt
     3001 ggatgagcgg ggaagggttc gggaagaagg ttgccgtcgt cacggcggcg gggagcggga
     3061 tgggcgcggc ctgcgccagg gagctctccc ggcgcggcta cagggtcgtc ctgatgtcgc
     3121 cctcgggccg ggcagaggac cttgccgacg agctcggggg tcttggcctg aggggctcgg
     3181 tcacggaggt gggggatctc gaatcgctcg tcggagaggc gctcgacagg tacggacgga
     3241 tcgacggcgt cgtcaacagc acgggccacc ctccctccgg ggagattctc gacctgaccg
     3301 acgaggagtg gtacgaggcc ctcgacctgg tgctgctgaa cgtcgtgcga accgcccgcc
     3361 tcgtgacgcc cacgatggcg agccagggag gtggtgccat cgtcaacatc tctacctact
     3421 ctgccttcga gccagccccg gagtttcccc tgtcttcctc cctccgcgcc gccctggcca
     3481 gcttcaccaa actttactcc aaccgctacg gggcagaagg catcaggatg aacaatatcc
     3541 tccctggcta catggagaac ttcgatatag acgaacagac cctgagctcc ataccaatgc
     3601 aacgccaggg ctcgctcgcc gagatagcag gaaccgcggc cttcttactc tccgacgccg
     3661 ccggctacat aacggggcag aacataaggg tagacggggg aataaccagg tcggtgtgaa
     3721 ggcttcagcc tttaggcttc aggtttcagg tcttcttacg gaaccgcttc ctccgtggcc
     3781 ggtttcaggt cttcccctac gtatcgagac tactggctga aagggagccc tgacagcgag
     3841 tttcagtgat gttggcccac atcgtatagg ttggttggcg ggctcatcga gaaatgggcg
     3901 tgcggagcgt cggggcggcc tacggcgcgc ctgaagcggc ttagaaactt cgaggaccgc
     3961 tacgacccga cgaacctttc ggatgaacgc gaacatcgaa ccccgagtcg acgaaagtga
     4021 cccttccgac gtgaaggatc ggcgtggatg ctgcccgcca gaaacgagtt aataacattt
     4081 aatacaaaca tcacccattt gggggatgca aaaagcgcca tcgaagacga tattggcttt
     4141 gtaagtaacg tttacaacaa ggagaagaga cgtgattacc gagtcgtcgg ccaccgctga
     4201 gctagtaggc gggttggaag ccctcgccac gctggctccg gagcgagccg tacggaaagc
     4261 cgtgcccttc cttacccggc tgacggatgg cgcggcctac ctggccgaac acgtcgcacc
     4321 cctctttcgg gaggccaagg acgccgacga atggtacgta gcccgtcgtt gggaaggaga
     4381 aggcgactcc tgttccctag aaatcttcgt ctggcccgcg ggaagcggta ccctgatcca
     4441 cgaccacacc tcctggggcg tcttccgctg cgtggtggga accaccctcg agaagcgcta
     4501 cgagcgactc gacgacggtt cgcgcttcga gcacgcgcgc ctgaagaagg cctggcagca
     4561 tgcctggggt ccggaggacg ggatctcgac ggtcctgccc ggcaacgggg gcatccaccg
     4621 ggtcgaaaac ccgggcgtga tcccggcgat aagcgtgcac ctctacggtc cgcggctcgg
     4681 ggagatggac gggcgagact acgacccctc ccgcgagtac gtgtgcgatc ggcgccccgc
     4741 ttgagttcgg gacgagccgg tcgcgattcc tgtgccgggg ccacgccctc ctgcacacct
     4801 tcgaaccaac catcgcgacc gggcacgcaa gcgtccccgt gatggataag agaatcgacc
     4861 gtgtcagaac aagagaacag gcgcaaggcc tgccgcatgc tggaggaggg cttcggacag
     4921 agcaaactgg aggtcttcga ctaggtcctg gatgccgact tcgtctgcta cgaccccaac
     4981 tctgaggctg gggaggcaag ggcgccgaca ccatcaagca agagatcgag tggttcagcc
     5041 gaacagggag ccaacgctat gatggcctcc gagcgtaaaa cgatcgtcgt taccggggcc
     5101 acgggccttc agggtggtgc cgtggctcgc cgcttgctcc tggacggttg gcatgtccgt
     5161 gggcttactc ggaatgccgc cagcaagcgg gccagggccc tcgaggagct cggcgccgag
     5221 gtcgcgcagg gcgatatggc cgaggcggcc tccctccgtc ccgtcttcga gggagcatac
     5281 ggcgtctaca gcgtccagaa ccccttcatc ggcgggccag agcaggaggt caggcaaggc
     5341 aagaacgtcg ccgaggtggc caaggacgca ggcgttgagc atctcgtcta cggttctgca
     5401 ggcaccggga ggaagggtac cggcgtcccc tcctgggaaa ccaagctgca gatcgaggat
     5461 cacataaagg cgcttgagct tccgcttacc atcctgcgtc ctacagcctt catggagttg
     5521 ctgacccaca agaagttctt tccgcccgtt gccgcgtggc aagtgatgcc gaagctgatg
     5581 ggatcatcgc ggcgtcttcc ctggctctgc accgaggatc tcggcgtcat cgccgcgaga
     5641 gcgttcgccg agccccgttc gttcgtcggg agggacctga cgctggccag cgacgtcaat
     5701 tctttagagg agtgccgctc ggagtatcgg gaggtgatgg gcacgaaccc gcggcgcttg
     5761 ccgatgcccg catggctgtt ccatcgtttc gggtttgtgg gcaaggattt gaccacaatg
     5821 tggcgatggc tcagaacggg cgatgtcgat ctggatacct cgcgtacgcg cgccatccat
     5881 ccggatgcgc tgacggtagg aggctggcta aggaaacaga aggcggcgga gtagtgggca
     5941 tccgggtcga aaggtcagct ggtgccgtgg ggtctttcgt cgatactcat agagcagccc
     6001 ctccatagcc gggaaggttt ccctattctg caccggacag gagcctattc acccgagtgc
     6061 gtggaaggat gattctccga cgtaggtcta gagaagggac cacctggctg gaaaagatgt
     6121 gccgggtggc gggtatgcat tgcttcgttt ctgtactaag atcctgtcta tcatcaagcc
     6181 gggtgctcct tatttccccc ctccaagagc agcccggctt ttctcgtgtc agggttgacg
     6241 tctggggtcg tgtgggacgg ggccccgcga agttcgcatg tacggcgctc caagaagttc
     6301 tcaatcgtct gcctgggaac gtcgtctgcc caactttgcg gaagggactt ccgtgtcgct
     6361 tcggagattc ccaatacggc gctctctcct tagcggcccc tcacccgctc gca
//
LOCUS       DBNGVD010000265         2213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_424567, whole genome shotgun sequence.
ACCESSION   DBNGVD010000265 DBNGVD010000000
VERSION     DBNGVD010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_424567"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16CM_07240"
     CDS             <1..759
                     /locus_tag="AC16CM_07240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3139660.1"
                     /translation="PLEATCARAEGPYRFVTLTDGSEVSCHALLIATGVSYRKLDVPG
                     VERLTGAGVYYGAAITEALACRDRDVFVLGAGNSAGQAAIHLSRYANSVTMLVRGASL
                     ARTMSQYLVSQIETHEKITVSPRTTVAGLTGGSSLEEIVIKDHTTGDVRSVPAFALFI
                     FIGATPHTEWVADVLERDSSGFILTGPGLLRGARRPRGWHLNRDPFLLETSVPGIFAA
                     GDVRHRSVKRVASAVGEGSMAVQFVHQYLSRVPE"
     gene            949..>2213
                     /locus_tag="AC16CM_07245"
     CDS             949..>2213
                     /locus_tag="AC16CM_07245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3139661.1"
                     /translation="MLDELRQVPLLADLSEEDLERLYEMAETVSVPAGQFVLREGDAG
                     DSLYIVLEGELEATRRRGGQEVVLSSYEPGQFFGEMALLEQAPRSASVRTLRESRVLA
                     IDQAAFRTLLSRSPSAPFNILRTVTSRLRSTESMLIQNEKMAALGTLAAGLAHELNNP
                     SSAIQRSVAQLRDALADLERSAAQIGSLSEEELGALRVEAMTRGPTTHSYDPLALSER
                     EDELQGWLEDHGVGQPWELAPVLASFGWTRDEVERLAIRFSPVKLPIVVRWLAATSSV
                     HGLLAEVGRGAEAIFEIVEAVKAYSYLDQAPIQEVDVRETLENSLVLLRPKLKMGVTI
                     RRDYADDVPRVEAYGSELNQVWTNIIDNAIDAVQGKGELELRTYPADGTVVIEITDDG
                     PGIPPEIRQRIFEPFFTTKGPGAGTGLGL"
BASE COUNT          409 a          675 c          718 g          411 t
ORIGIN      
        1 cccctggagg cgacgtgcgc gagggcagaa ggtccctacc gtttcgtcac cctcacggat
       61 gggtccgagg tcagttgcca cgccctgctt atagctactg gcgtgtcgta tcgcaagcta
      121 gacgtgcccg gcgtcgaaag gctcaccggc gcaggagtgt actacggggc cgctataacc
      181 gaggctctgg cctgtcggga cagggacgtg ttcgtcctcg gagcgggaaa ttcggccggt
      241 caggccgcaa tacacctctc gcggtacgcc aactcggtga ccatgctcgt cagaggagcc
      301 tcgctagcca ggacgatgtc ccagtacctc gtctcgcaga tcgagacgca cgagaagatc
      361 accgtgtcgc cgcgaactac ggtcgcggga cttaccggcg gcagcagcct cgaggagatc
      421 gtcatcaagg atcacaccac aggcgatgtg cgcagcgtcc ccgcgttcgc cctcttcatc
      481 tttatcggcg cgacgccgca tacggaatgg gtcgccgacg tcctggagag agattcgtcc
      541 ggctttatcc tgaccggacc cggcctgctc cgtggtgctc gtcgtcccag ggggtggcat
      601 ctcaaccggg accccttctt gctcgagacc agcgtgcccg gcatcttcgc agcgggggat
      661 gtgcgccaca ggtcggtcaa gcgcgtggca tcggcggtcg gggagggttc aatggccgtg
      721 cagttcgtcc accagtatct gagcagggta cccgagtagg atgtcgaggg ggttgaacag
      781 cctgacagag ctaattcttt atcatatcaa gaatgtctac cgtgtccgtc cgcccgaagg
      841 cttgtccttg gctcctggca aagctcgggc agcggggtca agccggtgca aaaacgtctc
      901 gcatgctcgg cggaagatgg gatctaggcc gggcgtccgc ggaggataat gctcgacgaa
      961 ttgcgccagg ttccccttct cgcagacctc tccgaagagg atctcgagcg actgtatgag
     1021 atggccgaga cggtttcggt tcccgcggga caattcgtgc tgcgggaggg cgacgctggt
     1081 gactcgctgt acatcgtcct cgagggcgag ctcgaggcga ccaggcgtcg gggcggccaa
     1141 gaggtcgtgc tgtcgtccta cgagccgggg cagttcttcg gcgagatggc cctgctggag
     1201 caggcaccac gttccgcctc ggtacgcacg ttacgggaga gccgtgtgct ggcgatagat
     1261 caagccgcct tccggaccct gctctcccgc agcccgtcgg cgcccttcaa catcttgcgt
     1321 acggtaacct ccaggttgcg cagtaccgag tcgatgctga tccagaacga gaagatggcc
     1381 gctctcggca cgctcgccgc ggggttggca cacgaactca acaatccgtc gtcggccatc
     1441 cagcgtagcg tggcgcagtt gcgcgatgcc ctggctgact tggagcgttc ggcggcccag
     1501 atcggctcgc tctccgagga ggagcttggc gctctgcggg tcgaagccat gacgcgtggg
     1561 cccacgacac attcctacga ccctctggcg ctcagcgagc gggaggacga gctgcaaggt
     1621 tggctggagg atcacggcgt cgggcagccg tgggaactcg cgcccgtgct ggcctctttt
     1681 ggttggacga gggatgaggt tgaacggttg gccatccgct tctctccagt gaagttgccc
     1741 atcgtcgttc ggtggctggc agccacctcc tcggtccacg gcctgctcgc cgaggtggga
     1801 agaggtgccg aagcaatatt cgagatcgtc gaggccgtca aagcatactc gtatctggat
     1861 caagcaccga tccaggaagt cgacgtgagg gagaccctgg agaactctct ggtgctcctg
     1921 cgtcccaagc tcaagatggg cgtgaccatc aggcgagatt acgctgatga tgtgccgcgc
     1981 gtagaggctt acggatccga gctcaaccag gtatggacca acattatcga caacgcgatc
     2041 gacgccgtac agggtaaagg cgaactcgaa ctacgcacct accccgcaga cggcacggta
     2101 gtcatcgaga tcaccgacga cgggccggga atccctccgg aaatccggca acgcattttc
     2161 gaacccttct ttaccacgaa gggtcccggc gcaggcacgg gcttgggact tca
//
LOCUS       DBNGVD010000266         3206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_970426, whole genome shotgun sequence.
ACCESSION   DBNGVD010000266 DBNGVD010000000
VERSION     DBNGVD010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_970426"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="AC16CM_07250"
     CDS             complement(<1..210)
                     /locus_tag="AC16CM_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139662.1"
                     /translation="MRTITIRYTAFRDCPNCGGRGDDGASWRPTECKRCKGKGRLVTG
                     VEVEDSLRGLLPDSSYDFNTGGEVFL"
     gene            247..663
                     /locus_tag="AC16CM_07255"
     CDS             247..663
                     /locus_tag="AC16CM_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3139663.1"
                     /translation="MVLLAVSAGMAIALQVTVNSIGLRDLGIGGLIGVSGLATAAVGF
                     LTTLMTERPEYTGKALLCALVSGVLGAFILGSIVLAAGQGGVARTLSLVIASQLIAGL
                     VIDRAGLLGAAPQEIGTSRILGIALILIGGVLVVRS"
     gene            complement(721..1971)
                     /locus_tag="AC16CM_07260"
     CDS             complement(721..1971)
                     /locus_tag="AC16CM_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3139664.1"
                     /translation="MIRESIVKDPSLAPEGHRKIDWVAQHAPVLNKIFGEQLADGSMR
                     GRKIAMTIHLEAKTAYLALLLSEAGADVTVAGSNPLSTQDDVCAALAERGVRVYATHD
                     PSDEDFELYLHNTLETGPDLLVDDGAELVGRLIEHHPDLAGNVIGASEETTTGVLKLK
                     AMTAEGVLPFPVLAANDARMKHLFDNRYGTGHSSIVALLSNTNLFLSGKAVVIMGFGW
                     VSRGLAKYADGMGGRVIVCEPDPVKLLEAYSEGYEVMNSLQAAEVGDVFLTGTGNLKV
                     LGVEHFALMKDGALLANAGHYDHEFDLASLREISTGSREVRRNVTEYDLPDGRRLHVL
                     ARGRLVNIAAGDGHPVEIMDLTFAVQALAAHHIANHADEMAPGIQPIPDEIDDLVARA
                     KLASLGVEPETLTEEQISYQQSWR"
     gene            2123..2422
                     /locus_tag="AC16CM_07265"
     CDS             2123..2422
                     /locus_tag="AC16CM_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139665.1"
                     /translation="MSYVTIQVIWLLCVLITPPALRLYPSKFWALPFCLVTVANGLHM
                     IFFRYEYNAVLRRAMRIIPYAQYLTPQREDPRYFLPLGGAYTIFGLVSIVLLIVA"
     gene            complement(2419..>3206)
                     /locus_tag="AC16CM_07270"
     CDS             complement(2419..>3206)
                     /locus_tag="AC16CM_07270"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3139666.1"
                     /translation="MIARDWPVNLAHRGASASAPENTLEAFRLALRSGAGGIELDVHL
                     THDGYPVVIHDPTLDRTTNLTGAVAEMGLDEVRWADAGYCFSTDGGKTYPYRSMGLQV
                     PTLAEVLRECPGVPVNIDMKADRPGIEAAVLGVLRRRDAAGRALVVSSRFGTVRRFRG
                     ISEGRISTGASRWETAVFLLASNLRLERLLVPDYDALQVPPRHRGIPLVTRRFVQAAH
                     ARNVRVDAWTINEKEQMRRLLDLGVDVIMTDRPEKLAEVLGKS"
BASE COUNT          530 a         1041 c         1001 g          634 t
ORIGIN      
        1 cagaaagact tcgccccctg tgttgaagtc atagctagag tctggcagaa gcccccgaag
       61 ggagtcctcg acctcgacac cggtcacgag ccgacccttt cccttgcacc tcttgcattc
      121 cgtaggacgc caggaggcgc cgtcatctcc gcggcccccg cagttggggc aatcccggaa
      181 agccgtatag cggatcgtta tagttctcat gtttcgaata ctatcaggga ggcaggcttg
      241 aactacgtgg tgctgctggc cgtatcggcg gggatggcga tcgcgttgca ggtgacggtc
      301 aactctatcg gcctcagaga cctcggaatc gggggtctta tcggggtctc cggtctcgcc
      361 accgcggcgg tggggttcct gacgacgctc atgacggaac gtcccgagta caccggcaag
      421 gcgttgctgt gcgcgctcgt ctccggcgtg ctcggggcct tcatcctcgg ctccatagtc
      481 ctcgctgccg gacagggcgg cgtcgcacgc acgctctcgc tcgtcatcgc aagccagctc
      541 atcgcgggcc tcgtcatcga tcgggccggt ctgctgggag cggccccgca ggagatcggc
      601 acatccagga tcctgggcat cgccctcatc ctgatcggtg gcgtcctggt cgtccggtca
      661 tgatccgctg taggggcgtt tcgcgaacca cccctacagc gggttcgttt gcgtcggggc
      721 ctaacgccag ctctgctggt aggagatctg ctcttcggtc aacgtctcgg gttctacgcc
      781 taacgaggcg agctttgcgc gggcgacgag gtcgtctatc tcgtcgggga tgggctgaat
      841 accaggggcc atctcgtcag cgtggttggc gatgtggtgg gcggcgagcg cctgtacggc
      901 aaaggtgagg tccattatct cgaccgggtg accgtcgccc gcggcgatgt tgacgagcct
      961 cccgcgcgcc aggacgtgca gcctcctgcc gtcgggtagg tcgtattcgg taacgttgcg
     1021 cctgacctcg cgagaacccg tcgaaatctc gcgcagggag gcgaggtcga actcgtgatc
     1081 gtagtggccg gcgttcgcga gcagggcacc gtccttcatc agggcgaagt gctcgacccc
     1141 gagtaccttg aggttgcccg tcccggtcag gaacacgtcc cccacctcag ccgcctgcag
     1201 cgagttcata acctcgtagc cttccgagta ggcttccagc agcttcacgg ggtcaggctc
     1261 gcagacgatg acgcgtcccc ccatgccgtc ggcgtacttg gccagcccgc gggagaccca
     1321 gccgaagccc atgatcacga ccgctttgcc cgagaggaag aggttggtgt tggagagcaa
     1381 ggcgactatg gaggagtggc ccgtgccgta gcggttgtcg aagaggtgct tcatcctggc
     1441 atcgttggcg gccagcacag ggaaaggcag cacgccctcc gccgtcatgg ccttcagctt
     1501 cagaacgccc gtggtggtct cctcggaggc ccctataacg ttgcccgcga ggtcggggtg
     1561 gtgttcgatc aggcgcccca caagctctgc accgtcgtcc accaggaggt cgggccccgt
     1621 ctccagcgtg ttgtgcaggt atagctcgaa gtcctcgtcg gaggggtcgt gggtggcgta
     1681 gacccgcacg ccgcgctcgg cgagggccgc gcatacgtcg tcctgggtgg agagcgggtt
     1741 gctccccgcc acggtaacgt ccgcccccgc ctcggagaga agcagggcca ggtaggccgt
     1801 cttcgcctcc aggtgaatgg tcatcgcgat cttgcgtccc cgcatcgagc catctgccag
     1861 ctgctcaccg aagatcttgt tcaggacggg tgcgtgttgg gccacccaat ctatcttgcg
     1921 gtgcccctca ggggcgagcg aagggtcttt gacgattgat tcccggatca tctctctcct
     1981 acctcgtctg cgtgctgcgg gccatgtctg gcccggtccc cacgagttta caatctgtat
     2041 gaacggaatg ctctcgtgta tgtccttaca ttagaatcac gagatcgacg gaagacacag
     2101 atacaggatc acgaccgcct ggatgagcta tgtgaccatc caggtgatct ggctgctctg
     2161 cgtcctgatc acgcccccgg ccctgaggct ttacccctcg aagttctggg ccctgccttt
     2221 ctgcctggta acggtggcga acggtctgca catgatcttc ttccgctacg aatacaacgc
     2281 ggttttacgc cgcgctatgc gtatcatccc ttacgcgcaa tacctgacgc cccaacgaga
     2341 ggaccccaga tacttcctgc ccctcggtgg cgcctacacg atcttcggcc tggtctccat
     2401 cgtgctgctc atcgtggcct agctcttacc cagcacttcg gccagcttct cgggcctgtc
     2461 ggtcatgatg acgtcgacgc cgagatctag caagcgtcgc atctgttctt tttcgttaat
     2521 ggtccaggcg tccaccctca cgttcctcgc gtgggccgcc tgaacgaagc gtcgggtcac
     2581 gaggggtatt cccctgtgcc tcggtggcac ctgcagcgca tcgtaatcgg gaaccaggag
     2641 ccgttccagg cgaaggttgc ttgcgagaag gaagaccgcc gtctcccacc tcgacgcccc
     2701 cgtagagatt cggccctccg agatcccgcg aaaacgccgc acggtgccga agcgggaaga
     2761 gaccaccaga gcccgtcccg ccgcgtcgcg cctgcgcagg acgccgagca ccgccgcctc
     2821 gatgccggga cggtcagcct tcatgtcgat gttgaccggg acgccgggac attcccgcaa
     2881 gacttctgcc agcgtgggta cctgcaaccc catactgcga tagggatagg tttttccgcc
     2941 gtcggtgctg aagcagtacc cggcatcggc ccagcgtacc tcgtcgagcc ccatctcggc
     3001 gacggcgccg gtcaggttcg tggtcctgtc cagggtcggg tcgtggataa ccacggggta
     3061 cccatcatgg gtcagatgta cgtcaagctc gatcccaccg gcgcccgatc gcaaggccag
     3121 ccgaaaagcc tcgagcgtgt tctccggcgc gctggcagaa gcgcccctgt gggccaggtt
     3181 cacgggccaa tcgcgcgcga tcatgc
//
LOCUS       DBNGVD010000267         3603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4980144, whole genome shotgun sequence.
ACCESSION   DBNGVD010000267 DBNGVD010000000
VERSION     DBNGVD010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4980144"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="AC16CM_07275"
     CDS             complement(<1..736)
                     /locus_tag="AC16CM_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3139667.1"
                     /translation="MDPKTIIVMEGDQTGQELLEEALRVLDPAVTGLDVEFVRYDLSL
                     ENRRATDNEVVHEAAAAMTESGFGIKAATVTPENPGDVGSPNAILRKGIDGTVIVRTG
                     RRIPGVNPTSGVHAPISVVRMAVDDAYGAKEWREGEGLDEVAFRTEKISRRVCRGVSE
                     FAFIQARKMRAKVFGGPKFTVSPVYEGMLKEEMDRAAENNRDVRYDPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMF"
     gene            952..1182
                     /locus_tag="AC16CM_07280"
     CDS             952..1182
                     /locus_tag="AC16CM_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139668.1"
                     /translation="MGTLLVVAAVVVVLVFAGVAFGTRGGRRDDDSPAAGGYYGPPGD
                     NGHHDHGGDGGDGGGWFDGGGGGDGGGGGSGN"
     gene            1437..1664
                     /locus_tag="AC16CM_07285"
     CDS             1437..1664
                     /locus_tag="AC16CM_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HIR3139669.1"
                     /translation="MGNKQDRDIEKIYSTPEFVAKLRRLADALEADERFEIQVAGERI
                     YVPVSAEFNIEHEREGNEEEIEFQLKWTNHS"
     gene            complement(1672..2631)
                     /locus_tag="AC16CM_07290"
     CDS             complement(1672..2631)
                     /locus_tag="AC16CM_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3139670.1"
                     /translation="MLFRQIECFVAVARLGNLSRAAEEMYLTQPTLTARLKALEEEVG
                     DSLFVRTSRGMRLTEAGREFLPYAERIVGGFDEGKRRLAELRGASGGRLRIGASPGVG
                     TYALPGLLERFAAAHPGVSISVRTGHSGAILEMTLSEEVQLGLTRDMRHPEVESLPLY
                     QDELVLVVDPGHRFTRRGVASLAEIGEEQLIFFDHDSSYFEQTHALFRNAGLGELRIM
                     EVDNIEAAKRMVEHRLGVAFLPRTAVVRSVTTGNLVLVSIEENPEMSRSIVALKRRDV
                     PATGPVAAFLEVADAMGEKRDGAQLSPTLATEFENLQLIDLFT"
     gene            2772..2981
                     /locus_tag="AC16CM_07295"
     CDS             2772..2981
                     /locus_tag="AC16CM_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139671.1"
                     /translation="MIPVQYRDPQTEEILERRYEEGAPAIGTRVRIGFEEFRVLYRWR
                     CVPTSCIVYVHRVARERRQAVRAAA"
     gene            3059..>3603
                     /locus_tag="AC16CM_07300"
     CDS             3059..>3603
                     /locus_tag="AC16CM_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR3139672.1"
                     /translation="MAEGTRTYAEGIEFTTGIPEEFEEILTPEAVAFVAKLSREYRGR
                     VVELLDKRAERQERINAGEMPDFLPETREVREGDWKIAPIPDDLQDRRVELTGPPDRK
                     MTINALNSGASCWMADFEDANCPTWHNMLESQLNMKQAIEGTISFDDPGSGKHYELGE
                     DTAVILARPRGWHLFEKHMLV"
BASE COUNT          719 a         1145 c         1124 g          615 t
ORIGIN      
        1 cgaacatctg catgaccagg tcagagatgc agtcgccgtc gcggttgagc gtgggtatga
       61 cgagcggctc gccgtaggta gtgagcagca gcgcgtaggt ggcgtctatg agctgggggt
      121 cgtagcgtac gtcgcggttg ttttcggcag cccggtccat ctcctctttg agcatcccct
      181 cgtagacggg agagaccgta aacttgggac cgccgaacac cttggcccgc atcttgcgcg
      241 cctggatgaa ggcgaactcg gagacgcccc ggcaaacgcg acgcgagatc ttctccgtcc
      301 tgaaagctac ctcgtcgagt ccctcgccct cgcgccactc ctttgcgccg taggcgtcgt
      361 cgacggccat gcgcacgacg gagatgggcg cgtgaacccc gctcgtcggg ttgacgccag
      421 ggatgcgccg ccccgtgcgc acgatgacgg tcccgtcgat gcccttcctc aagatggcgt
      481 tgggcgaacc cacgtcaccg gggttctctg gcgtaacggt cgccgccttt atgccgaagc
      541 ccgactcggt catggccgcg gccgcctcgt gcacgacctc gttgtccgtt gcccgcctat
      601 tctccagcga gaggtcgtag cgcacgaact ccacatcaag gcccgtaacc gcgggatcca
      661 gcacccgcaa agcctcctcc agcagctcct ggcctgtctg gtcgccctcc ataacaatta
      721 tagtcttggg gtccaatcgt cgcacctctg caattagtcg tatcggatat ccgtctagag
      781 tatacgaccc acactagaac ccccgcacac caccatcccc cacacccctg tatagggcgg
      841 ttcgaaaccc gccaggcaac aacctgcacg tcctcaccca gacggttagc acggaccgca
      901 caagggtaga atcatgcaga ggtttgcaag atccaggcag gggggttctg agtgggaacg
      961 ttactggtgg ttgcggcggt agtagtggtg ctggtgttcg caggtgtcgc cttcggcaca
     1021 cgcggtggcc gcagggacga cgacagcccg gccgccggcg gctactacgg acctcccggg
     1081 gacaacggcc accacgatca cggcggagat ggtggggatg gcgggggctg gttcgacggt
     1141 ggaggcggcg gtgatggcgg cgggggtgga agcggcaact gaaaccgtca gcaacgcgaa
     1201 tagcattgga aaccttctca tcgcggccag acctttctac acctttctac gggagagtag
     1261 gcctcctgtt acgaacaccg atcctgagga ctcgctcaga agcccggcca ccatgcggag
     1321 gcgggatctt cacccaccgt cgggagagac cttgcccacc tcaaagctgt tcgccgcgga
     1381 atccctggct ctgtaagcta gaatatgcgt aatggtccac tagtcaggga ggagtaatgg
     1441 gcaataagca ggacagggac atagagaaga tctactccac gccggagttc gtggcgaagc
     1501 tgcgcaggtt ggccgacgcg cttgaggctg acgagcggtt cgagatccag gtagcggggg
     1561 agaggatata cgtgccggtc agcgccgagt tcaacatcga gcacgagcgc gaggggaacg
     1621 aagaggagat cgagttccaa ctcaagtgga ccaaccactc ctgaccttcc ctcaagtaaa
     1681 caggtctatg agctgaaggt tctcgaactc cgtggccagg gtgggggaga gctgggcccc
     1741 gtcccgcttc tcgcccatcg cgtcggcgac ctcgagaaag gcggccaccg gccccgtggc
     1801 aggcacgtcg cggcgcttga gggcgacgat ggaacggctc atctccgggt tctcctcgat
     1861 ggataccagg acgaggttcc ccgtggtgac ggacctgacc acggccgtcc ggggcaggaa
     1921 ggcgaccccg agcctgtgct ctaccatccg cttggccgcc tcgatgttgt ccacctccat
     1981 aatccttagc tccccgagcc cggcgttcct gaacagcgcg tgcgtctgct cgaagtagct
     2041 ggagtcgtgg tcgaagaaga tgagctgctc ctccccgatc tccgccaaac tcgctacccc
     2101 acgccgggta aacctgtgcc cgggatctac gacgagcacc agctcatcct ggtacagcgg
     2161 caaactctcc acctcgggat ggcgcatatc ccgcgtgagg ccgagctgga cttcctcgct
     2221 caaggtcatc tccagaatgg ccccggaatg ccccgtcctc accgagatgg aaaccccggg
     2281 gtgcgccgca gcaaaccgct ccaataaacc aggcaacgca taggtcccaa ctcccggcga
     2341 agccccgatc ctgagccgtc ccccagaagc ccccctgagc tccgccaacc gccgcttccc
     2401 ctcatcgaac ccgccaacga tcctctccgc atacggtaga aactccctgc ccgcctccgt
     2461 aagccgcatc ccccgactgg tcctcacgaa cagagagtcc ccgacctcct cctccagcgc
     2521 cttgagccgc gccgtcaaag taggctgcgt gaggtacatc tcctcagcag cccggctcaa
     2581 gttgccaagc cgcgccaccg caacgaaaca ctcgatctgc ctgaaaagca cggggtgact
     2641 cctttcagta gccagctgtc agctattagc tataagtttc tcctatatca acaatacgtg
     2701 caaacaattg gaataatacc tggggtgttg gtatcttgct ttcacggcag ggcgaccgaa
     2761 aggtggaggc tatgatcccg gtacagtaca gagacccgca gacggaggag atcctggagc
     2821 gccgttacga ggagggcgcg ccggctatcg ggacgcgggt gaggataggg ttcgaggagt
     2881 tccgggttct ctacaggtgg cgttgcgtgc cgacctcctg catcgtttac gtgcacaggg
     2941 tcgccaggga gcgtcgtcag gcggttcggg ctgctgccta ggctcggctc cgagcgttcc
     3001 ggggcgtggc ttgctagagt agccacgctc gggaagctgt agagaggagc cggaggtcat
     3061 ggccgaagga acacgcacgt atgccgaggg gatcgagttt acgaccggga tccccgagga
     3121 gttcgaggag attctgacgc ccgaggcggt cgccttcgtg gcgaagctct cgcgcgagta
     3181 cagggggagg gtggtcgaac tcctcgacaa gcgggcggag cgccaggaga ggatcaacgc
     3241 gggcgagatg cccgactttc tgccggagac gcgggaggtc agggagggcg actggaagat
     3301 agcccccatc cccgacgacc tgcaggacag gcgcgtggaa ctcacgggac cgcccgaccg
     3361 caagatgacc ataaacgcgc tcaactcggg ggcgagctgc tggatggccg acttcgagga
     3421 cgccaactgc ccgacctggc acaacatgct ggagagccag ctaaacatga agcaggccat
     3481 agagggcacc atctccttcg acgacccggg ctcgggcaag cactacgagc tcggcgagga
     3541 cacggccgtc attctggcca ggccccgcgg ctggcacctc ttcgagaagc acatgctcgt
     3601 gga
//
LOCUS       DBNGVD010000268         4005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_425917, whole genome shotgun sequence.
ACCESSION   DBNGVD010000268 DBNGVD010000000
VERSION     DBNGVD010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_425917"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16CM_07305"
     CDS             complement(<1..400)
                     /locus_tag="AC16CM_07305"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR3139673.1"
                     /translation="MQGARTSRFARPQRVLGVMVLAGATVALIFVASVFGYGVFAQDS
                     GDTTGDRAKNVIFVLGDGLGLAQRDAIQLSKVGPYDRLAMDRLPYQGLSNTNAEDPET
                     FVTDSAAGATALATGVKTYNGSINVDNGGDK"
     gene            complement(602..1597)
                     /locus_tag="AC16CM_07310"
     CDS             complement(602..1597)
                     /locus_tag="AC16CM_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3139674.1"
                     /translation="MSLALIAVALAYAFDFANGFHDTANAVATIIYTKAMKARTAIIM
                     SGLLNFLGAVVVGTAVAVIITEVVPADVVTIRLILAALMGGLVWNLGTWYYGLPVSSS
                     HTLVGSLVGAGLAASWLSGVQWGALTEIILALLVSPVLGIGLAWMVTRLTLWISGRRK
                     TASTAHPKALRWMTILSGAAVSFAHGSNDGQKTMGVITLVLIAEFGHPESAGVPFWVI
                     VTAALAIGLGTMIGGWRIIRTVGEKISQVGITPVQGFSAQLSAALIILIGSRIGAPVS
                     TTHVLSSAVAGSTVGQHTHKGLNLKVASSIFVAWLVTLPATAVMAAVIYLLLGLI"
     gene            complement(1718..2992)
                     /gene="serS"
                     /locus_tag="AC16CM_07315"
     CDS             complement(1718..2992)
                     /gene="serS"
                     /locus_tag="AC16CM_07315"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3139675.1"
                     /translation="MLDLRFIRENVEAVEENCRNRGVEADVGLVIELADRRSALIQEL
                     NELRQRQNEMARSVGKERDEERRGRLIEESRAMKERLPSKEGELHEVEDRLRDEQSKI
                     PNMTHPDSPIGRDDTENVEIRRWGEIPNFSFEPKDHAEIGEALGIIDFDAGARTTGSK
                     FYFLRGDAVLLELGLIRYALDIIMESGYQPTITPDLARDQMLEGTGYIPRGPETQIYS
                     VEDSDLSMIATAEIPLAGQHAEQILDEAALPLRYAGLSHCFRTEAGSHGRASRGLYRV
                     HQFTKVEMFAFTTPEGSEDIHNEMVEIEEQIFQGLGLPYRVVDICTGDLGGAAYRKYD
                     LEAWMPGRNEFGEVTSTSNTTDYQARRLAIRFRKEGGRPQLLHTLNGTAIAMSRALIS
                     LLEIYQEEDGSVTLPEVLFPYVGRERLVAVAT"
     gene            complement(3066..3593)
                     /locus_tag="AC16CM_07320"
     CDS             complement(3066..3593)
                     /locus_tag="AC16CM_07320"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HIR3139676.1"
                     /translation="MVGSLSVVYLIVGVCLVALQRLLELVYSRRNERRLRARGAVERG
                     AGHYLLMVGLHVLWLVSTLVEGLLRGPEIPVWWPLPLAAFLLVQPLRYWAIVSLGTNW
                     NTRVLVVPGMKPVKAGPYRYLSHPNYVVVAVEILAFPLIFGAWLTAVFFSLLNAALLF
                     VRIRTEDRALREHAG"
     gene            complement(3655..>4005)
                     /locus_tag="AC16CM_07325"
     CDS             complement(3655..>4005)
                     /locus_tag="AC16CM_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014815.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HIR3139677.1"
                     /translation="KVQLSKSVPAIVQARFRENLEEACESLGLDFDELEHFVLHPGGS
                     KVLDAFENVLGLESGGLATSRDVLRDCGNMSSVTVLFILERFLESREFTRGELGVLSA
                     MGPGFSAEHVFFRC"
BASE COUNT          746 a         1282 c         1243 g          734 t
ORIGIN      
        1 gcttgtcacc cccgttgtcc acgttgatag aaccgttgta cgtcttgacg ccggtggcga
       61 gcgcggtcgc cccggcggcg gagtctgtaa cgaaggtctc cggatcttcg gcgttggtgt
      121 tgctcaaacc ctggtagggc aggcggtcca tggcgagcct gtcatagggt ccgaccttcg
      181 agagctgtat ggcgtctctc tgtgccagcc ccaggccatc gcccaacacg aagatcacgt
      241 tcttcgccct gtcccccgtc gtatccccgc tatcctgcgc gaacaccccg taaccaaaga
      301 cgctggccac aaagataagg gctaccgtcg cgcccgcaag caccataacc ccaagcaccc
      361 gttgcggtcg cgcgaacctg ctcgttcttg ccccttgcaa ttcccctcct cagttagtct
      421 gccttctact cctcacttgc cgacccgcag tgtacagtct tctcgcccgg cacaaaagta
      481 acgggacata cgtaagcggt ctctggcggc gatagagatc gtggactgcg gccccgttcg
      541 tggagcatct gcagggctcc ctccggtaat cgcgggttgc gacctacgtc ctcatcgggc
      601 cctagatgag gccgaggagg aggtagataa cggctgccat cacggcggta gcagggaggg
      661 ttaccagcca agccacaaag atgctcgaag cgaccttgag gttgaggccc ttgtgcgtgt
      721 gctggccgac ggtggacccg gccacggccg acgaaagcac gtgcgtggtc gagacgggcg
      781 cgccaattcg gctgccgatc aggatgatca gggccgccga gagctgggca gagaagccct
      841 gcacgggcgt gatcccgacc tggcttatct tctctcccac ggttctgatg atgcgccagc
      901 ccccgatcat ggtgccaagc ccgatggcta gggccgccgt cacgatcacc cagaacggca
      961 ccccggctga ttcggggtga ccgaactctg ctatgagaac gagcgttatc acgcccatgg
     1021 tcttctgccc gtcgttggag ccgtgcgcga aagagaccgc cgcgcccgag aggatcgtca
     1081 tccaccgtaa agccttcgga tgggcggtag aggctgtttt tctccgtccg gagatccaga
     1141 gcgtaaggcg ggtaaccatc caggccaggc ctatgcccag caccggtgag acgaggaggg
     1201 cgaggatgat ctccgtcagc gccccccact ggacgccgga gagccagctc gcggccagac
     1261 cggctccgac caggctgccg accagggtat gtgagctgga gacggggaga ccgtagtacc
     1321 aggtccccag gttccacact aacccaccca tcagggcggc aaggatcagc cttatggtta
     1381 ccacgtccgc cggaacaacc tcggtgatga tcaccgccac cgccgttccg accacgacgg
     1441 ccccgaggaa gttgagcagg ccgctcatga taatggcggt tctggccttc atcgccttgg
     1501 tgtagatgat cgtcgccacc gcgttggctg tgtcgtggaa accgttggcg aaatcgaagg
     1561 cgtaggccag cgccacagcg atgagcgcta gagacacgcc tgactccagc gcacgccgtc
     1621 cccttcccta ccctgaccac tgttcccgat caccgcgcgt atattctcat aatctctggg
     1681 ctcgtgtatg gcgcaggacg aggtcctgct atttcggcta ggttgctacg gccacgagcc
     1741 tctctcttcc cacataagga aagaggacct cggggagggt aacggagccg tcttcctcct
     1801 ggtagatctc gagaagcgag atcagggccc tgctcatggc gatggccgtg ccgttgaggg
     1861 tatgcagtag ctgaggcctt ccgccttctt tcctgaagcg gatggcgagg cggcgggcct
     1921 ggtagtcggt ggtgttggag gtgctcgtta cctcaccgaa ctcgttccgg ccaggcatcc
     1981 aggcctccag atcatacttt cggtaggcgg caccaccgag gtcgccggta cagatgtcga
     2041 ccacgcggta aggcaggccg aggccctgga agatctgttc ctctatctcg accatctcgt
     2101 tgtggatgtc ttccgaccct tcaggtgtag tgaaggcgaa catctccacc ttggtgaact
     2161 ggtgtacgcg gtacaggccg cggctcgcgc ggccgtgcga gcccgcctcg gtgcggaagc
     2221 aatgggacag gccggcgtag cgtagcggga gggcggcctc gtccaggatc tgttcggcgt
     2281 gctggccggc gagcgggatc tcggcggtgg cgatcatgga caggtcggag tcttcgaccg
     2341 agtagatctg ggtctccggc ccgcgcggga tgtaccccgt cccctccagc atctggtccc
     2401 gagccaggtc gggggttatg gtgggctggt acccactctc catgatgatg tcgagggcgt
     2461 agcggatcag gccgagttcc agcagcacgg cgtccccgcg caaaaagtag aacttgctac
     2521 ccgtggtccg ggcgccggcg tcgaaatcta tgattccgag cgcctccccg atctcggcgt
     2581 ggtccttcgg ctcgaaggaa aaattcggga tctcgcccca gcgtcggatc tcgacgttct
     2641 cggtgtcatc cctgccgatg ggcgagtccg ggtgggtcat gttggggatc ttggactgct
     2701 cgtcgcgcaa cctgtcctcg acctcatgca actccccttc cttggagggc agccgctcct
     2761 tcatagcacg cgactcctcg atgagccgtc cccgcctctc ctcgtcgcgc tccttgccta
     2821 cgctcctggc catctcgttc tgtcgttgcc tgagctcgtt caactcctgg atgagggcag
     2881 agcggcggtc ggccagctcg atgacaagcc ccacgtcggc ctcgacgcca cgattccgac
     2941 agttctcctc gacggcttcg acgttctcac ggatgaatct gagatcgagc acgggggcct
     3001 cccttcggtc ggcaggtatc aggcttcagg ttcaaggcat cagccagggg ctgatcggtc
     3061 ttatcttagc ctgcatgctc ccggagtgcc cgatcctctg tccggatgcg gacgaagagg
     3121 agcgcggcgt tcagcagcga gaagaagacc gcggtgagcc aggccccgaa gatgagcggg
     3181 aacgccagaa tttctacggc aaccacgacg tagttgggat gggaaagata gcggtaaggc
     3241 ccggccttga cgggcttcat accgggcacg acgagcaccc tggtgttcca gttcgtgcca
     3301 agggacacga tcgcccagta ccgtaacggc tgtacgagca gaaaagccgc cagcggcaag
     3361 ggccaccaga cgggtatctc gggaccgcgc aagagtccct ccacgagcgt cgagaccagc
     3421 cagagcacat gaagcccgac catcaggaga tagtggccgg ccccccgctc cacggcacca
     3481 cgggctcgca accggcgctc gttacgccgc gaatagacga gctccagcag ccgctgaagt
     3541 gcgacgagac acacccccac aatgaggtag acgacgctca atgagccgac caccccaccg
     3601 gataaccctg atgcctgatg cctgatgcct gatgcccagg atgctgacct gcggtcagca
     3661 tctgaaaaac acgtgctccg ctgagaagcc cgggcccatc gccgagagca cgccgagttc
     3721 gcccctcgtg aactcgcggc tttcgaggaa acgctccagg atgaagagca cggtgacgct
     3781 cgacatgttg ccacagtcgc gcagcacgtc cctgctggtg gcgagccccc cagattcgag
     3841 gccgagcacg ttctcgaagg cgtcgagaac cttggagccg ccggggtgga gcacgaagtg
     3901 ctcgagctca tcaaaatcga ggccgaggga ctcacacgcc tcttccaggt tctcgcggaa
     3961 cctggcctgc acgatggcgg ggacgctctt ggagagctgg acttt
//
LOCUS       DBNGVD010000269         4350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1518061, whole genome shotgun sequence.
ACCESSION   DBNGVD010000269 DBNGVD010000000
VERSION     DBNGVD010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1518061"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /locus_tag="AC16CM_07330"
     CDS             <1..2229
                     /locus_tag="AC16CM_07330"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR3139678.1"
                     /translation="WWLYGAALAAGGAFSILFNGETYRVGDGGFGDLSVVSLIAGDRA
                     FLPLLLAAVVAALVGSAGRWRFLLVVPATVVYTIVAVYGLEFLPELFSVSGWREISVV
                     LGRDVYEAANIMYAEPIPYSLAPGLFLILIPVVMILVAFATSATIYERSPVISMAVLG
                     LTIGVLSTISFEDGAGIFFAVFLACTVGLLLSLDRAGPEDPGRPAIAAGVFVVALVLL
                     IPRLPFSDETVSPGAIDWTRIGTGGTSRLDVQADVGDYLTAGRDAELMRVQSTEPLLW
                     RGGTMDYFDGVRWLDTTRPGDDDGEEISPGVETRPVVQRVQVLNARTDIIFGGYRIVE
                     TSEPFGAAVQNSDGSWSMETPFEEGDSYQLLSQVPQPTAAQLRSAGVDYPSVVTQKFL
                     QLPDDTPPVVAQTADRIERRYDPQTPYDEARAIEQYLTYDGGFTYNLDVSYRRADKAI
                     EEFLGDGKEGFCTQFSTSMALLLREMGVPSRVVYGSTSGEEVGRNEYLVKGSNMHTWV
                     EAYFPGVGWYPFNPTPGFSMPAAMEANAPRPDLPLADQGSQSENDNLDQRRSERERAQ
                     QTPQEQNENPTDGNPTGNPGDVAIPVWPLLVLLPVLLVAAVPLSKRALLARGKPENLY
                     RDLTGRLRDVLPPGKSHVADSPALTPTERMLLLAGAAGVEEEPPTRFARAYSEYLYSA
                     HADEERLTTDYQDAIEAYVRLPGWRRALGALNPASLLVRAGAYASARKTRLVKKLRGK
                     LR"
     gene            2302..2676
                     /gene="erpA"
                     /locus_tag="AC16CM_07335"
     CDS             2302..2676
                     /gene="erpA"
                     /locus_tag="AC16CM_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3139679.1"
                     /translation="MTTTAREVLMQQKEETTIVAITDNAAEKVKALMAQEGEEDLALR
                     IGVRPGGCSGFQYSIYFDDEIADDDEVLETKGVRVLVDAMSVPYIMGSEFDYVDGLMG
                     AGFAVNNPNVQGGCGCGSSFTC"
     gene            complement(3057..3620)
                     /locus_tag="AC16CM_07340"
     CDS             complement(3057..3620)
                     /locus_tag="AC16CM_07340"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139680.1"
                     /translation="MSQGFSILDLAAEDAEAVGQAARLLVEGFRDDWPGAWPNEEEAL
                     QEVREALGADRICRVARGEDGTVLGWIGGIPGYEGKVWELHPLVVRSDVRRRGVGRVL
                     VSDLEARVRERGALTLWLGTDDVTGMTTLSGKDLYPDVLGHLENIQNLRGHPYEFYSR
                     LGYSLIGAMPDANGWGKPDIFMAKRLQ"
     gene            complement(3617..4174)
                     /locus_tag="AC16CM_07345"
     CDS             complement(3617..4174)
                     /locus_tag="AC16CM_07345"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3139681.1"
                     /translation="MTEGSEGWERLGSERLLSNRYFTLRSDRLRLPDGGIKDPYFVLE
                     RPDAAIIVPITEEDEVVLVRQYRPPLRMMELGLPAGLVEAGERPEEAARRELAEETGH
                     TGGGWELLGSLASSPSLKDNWAYLFLARGVEETAAPAPDEHEMVEVVKVPVEDLRGLV
                     RSGEIVSSSGVAAIMLALERLREDK"
     gene            complement(4167..>4350)
                     /locus_tag="AC16CM_07350"
     CDS             complement(4167..>4350)
                     /locus_tag="AC16CM_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139682.1"
                     /translation="EEALRDVPTVGFLSHVQKDLAVAAREAGCDRIVARSAFTRNLPR
                     ILSGDTPDTIDEAGVD"
BASE COUNT          742 a         1477 c         1379 g          752 t
ORIGIN      
        1 tggtggctct acggggcggc gctcgccgca ggcggcgcgt tcagcatcct gttcaacggc
       61 gagacctacc gtgtcggcga cggggggttc ggagatctct ccgtggtctc cctcatcgcc
      121 ggggacaggg ccttcttgcc gctgctcctc gcggccgtcg tggcggcgct ggtgggctcg
      181 gcgggcaggt ggcgcttcct tctcgtcgtt cccgccacgg tcgtctacac catcgtcgcc
      241 gtgtacggcc tggaatttct ccccgagctc ttctcggtct cggggtggcg cgagatctcc
      301 gtcgtcctgg gccgcgacgt ttacgaggcg gcgaacatca tgtacgccga gccaataccg
      361 tacagcctgg cccccggcct cttcctcatc ctcatacccg tggtcatgat cctggtcgcc
      421 ttcgccacct cggcaaccat ctacgagagg agccccgtca tctccatggc cgtgctcggg
      481 ctcaccatag gcgtgctctc gaccatcagc ttcgaggacg gggccggtat cttcttcgcg
      541 gtgttcctgg cgtgcaccgt gggcctgctc ctctccctag atagggccgg gccggaggac
      601 cccggccgcc ccgccatcgc ggccggggtc ttcgtggtcg cgctcgtgct cctcatcccc
      661 aggttgccct tctccgacga gacggtcagc cccggtgcga tagattggac gcgcataggg
      721 acgggcggca cctccaggct cgacgtccag gccgacgtgg gggactacct cacagcaggt
      781 agggacgccg agctcatgcg cgtccagagc acggagccac tgctgtggcg cggcgggacc
      841 atggactact tcgacggcgt gaggtggctg gacacaaccc gtcccggaga cgacgacggg
      901 gaggaaatct cccccggcgt cgagacccga cccgtggtgc agagggtgca ggtgctcaac
      961 gcccgcacgg acataatctt cggagggtac agaatagtcg agacctccga gcccttcggg
     1021 gctgcggtac agaactccga cggctcgtgg tctatggaga cacccttcga ggaaggcgac
     1081 tcctaccagt tgctctcgca ggtgccccag cccaccgcgg cgcagctcag gagtgcaggc
     1141 gtcgactacc cttctgtcgt gacgcagaag ttcctgcagc tcccggacga tacaccgccc
     1201 gtagtcgcgc agacggccga ccggatcgaa cgcagatacg acccccagac gccctatgac
     1261 gaggcgcgcg ctatcgagca gtacctgacc tacgacggcg gctttaccta caacctggac
     1321 gtgagctacc gccgggccga caaggcgata gaggagttcc ttggcgacgg caaggagggc
     1381 ttctgcaccc agttctcgac ctcgatggcg ctcctcttgc gcgagatggg cgtgccctcg
     1441 cgggtggtct acggttccac ctcgggcgaa gaggtgggca ggaacgagta cctggtcaag
     1501 ggctccaaca tgcacacctg ggtggaggcc tacttcccgg gggtcggctg gtaccccttc
     1561 aacccgaccc cgggcttctc catgccagcc gcgatggagg cgaacgcccc acgccccgac
     1621 ctcccgctgg ccgaccaggg cagtcaatcg gagaacgata acctggacca gcgcaggtcc
     1681 gagcgcgaga gggcgcagca gacgccccag gaacagaacg agaaccccac agatggcaac
     1741 cccacgggca accccgggga tgttgccatc cccgtgtggc cactgctcgt cctgttgccc
     1801 gtgctgctcg tggccgccgt acccctctcc aaacgcgcgc tcctggcccg gggcaagccc
     1861 gagaacctct acagggacct caccggccgc ctgcgcgacg tgctaccgcc gggcaagagc
     1921 cacgtcgccg actcgccggc gctgaccccg accgagcgca tgttgctcct cgcaggggca
     1981 gccggcgtgg aggaggagcc cccgacccgt ttcgcccggg cctactccga gtacctctac
     2041 tcagctcacg ccgacgagga acgtctcact accgactacc aggacgcaat agaggcctac
     2101 gtaaggttgc cgggctggcg cagggcgctc ggggccctca acccggcctc gctgctcgtc
     2161 cgcgccggag catacgcctc cgcccgcaag acacggctcg tcaagaagtt gcgcggaaag
     2221 ctccgatagc tgaaagctga tggctgacag ctttcaagcg ttgaagtata cggtttgcgg
     2281 tgtaataatt tccctaccaa catgacgact acagcacggg aggtgctgat gcagcagaaa
     2341 gaagagacta ccatcgtcgc catcaccgac aatgccgcag agaaggtcaa ggccctcatg
     2401 gcccaggagg gcgaagagga tctcgcgctg cgcatcggcg tcaggcccgg cggctgctcc
     2461 ggtttccagt acagcatcta cttcgacgac gagatcgccg acgacgatga ggtcctcgag
     2521 accaagggcg tcagggtgct cgtcgacgcc atgagcgtcc cctacatcat gggcagcgag
     2581 ttcgactacg tggacggcct catgggtgcc ggcttcgccg tgaacaaccc caacgttcag
     2641 ggtggctgcg gttgcggtag ctcgtttacc tgttagttag ctgttagctt tcagccttca
     2701 gctgtcagct ctcgaatacc cagggcccgg ggcactacgc tccgggcctt atttgtgctg
     2761 atgccgaatc tggttgcttg acaacggtgg gctatcatcg cgcgggccta cgtctcctaa
     2821 tacccgtgga ttccagcttt cgcgggaatg acggatgaga ggtactcggc gtgcgccaac
     2881 agtcggaaag cgtaaccgtt cggacaaggt gagtttgaat ccggtcgaga gtaaaggtgc
     2941 tcaagcggcg aacaagacga ggcaccacct tctgcgtcat tcccgcgaaa gctggaatcc
     3001 acgagttgca aagacgtggg gcctgatgga gggtggtatc gctgtttgcc cggtgtttac
     3061 tggaggcgtt tggccataaa gatgtcgggt ttgccccagc cgtttgcgtc tggcatcgcg
     3121 ccgatgagcg agtaaccgag cctggagtag aactcgtagg ggtgtccgcg caggttttgt
     3181 atgttctcca ggtgacccag aacgtcgggg tagagatcct tgccggacag ggtggtcatg
     3241 cctgttacgt cgtccgtgcc gagccagagc gtcagggcac cacgctccct gacacgtgcc
     3301 tccaggtcgg agacgagcac gcgtccgacg cccctgcgcc tgacgtcgga gcgcaccacc
     3361 agcgggtgca gctcccagac cttgccctcg taacccggta tgccgccgat ccaacccagc
     3421 accgtgccgt cctcgccgcg ggctacccgg cagatcctat ccgcgcccag ggcctcacgc
     3481 acctcctgca gggcctcctc ctcgttcggc caggcccccg gccagtcgtc cctgaacccc
     3541 tcgaccagga gcctcgccgc ctgcccaacg gcctccgcgt cctctgcggc gaggtcgagg
     3601 atggagaacc cctggctcac ttgtcttccc gcaaccgctc cagggcaagc atgatggcgg
     3661 cgacgccgct cgagcttact atctcgccgg agcgtacgag gccgcggagg tcttctacgg
     3721 gaaccttcac cacctcgacc atctcgtgct cgtcgggggc cggggcagcg gtctcctcga
     3781 cgcctcgggc gaggaagagg taggcccagt tgtccttgag gctgggggag gaggccaggg
     3841 agcccagaag ctcccacccg cccccggtgt ggccggtctc ctcggctagc tcgcggcggg
     3901 ccgcctcctc cgggcgctcg cccgcttcga ccagacccgc cggaagcccg agctccatca
     3961 tccggagcgg cggacgatac tgccgaacga ggacgacctc atcctcttcg gtgatcggca
     4021 cgatgatcgc ggcgtcgggg cgctccagca cgaagtaggg gtctttgatc cccccgtcag
     4081 gtagccgcag ccggtccgag cgcaaggtga agtacctgtt ggagaggagc ctctcgctgc
     4141 cgagccgctc ccaaccctcg gacccctcag tcaacgccgg cctcgtcgat ggtgtctgga
     4201 gtatcccctg acagtatcct cggcaggttc cgcgtgaagg cgctgcgggc gacgatgcgg
     4261 tcacagcccg cctctcgggc ggcgaccgcc aggtccttct gtacgtgcga gagaaagccg
     4321 accgtgggga cgtctcgcaa agcctcctcg
//
LOCUS       DBNGVD010000270         2868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2065140, whole genome shotgun sequence.
ACCESSION   DBNGVD010000270 DBNGVD010000000
VERSION     DBNGVD010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2065140"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC16CM_07355"
     CDS             complement(<1..238)
                     /locus_tag="AC16CM_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3139683.1"
                     /translation="MDTRELDQQRAEAFAGRMIGTLNEGAIAVMISIGHRTGLFDAMS
                     GLSPSTSGEIADAADLSERYVREWLGAMTVGGIVE"
     gene            complement(266..1675)
                     /locus_tag="AC16CM_07360"
     CDS             complement(266..1675)
                     /locus_tag="AC16CM_07360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3139684.1"
                     /translation="MTVGGEESSLHGAEVERLASGLEGDLFRPVDAGYEEARRLWNGV
                     IDRRPALIARSTNVEDVVAVVSFARENGLLLAVRGGGHNVAGTASAEGGLVLDLSAMK
                     DIEVDPAARTVRAGAGVIIGELDAETQKHGLATPMGVVSETGIAGLTLGGGVGHLRRK
                     HGLSSDNLLSVELVTADGRVLTASKEENADLFWAVRGGGGNFGVITSFEYRLHPVGPE
                     VMCAFVFYPGDRSEEVLRSIEAYVAGAPNGVSPLSFLGRVPRVYDFPAEWPGEQFVAT
                     LAVYDGPVEEGAEVLEPLRVLGDPIAEFSGPMPYTEVQKLLDEDYPDGWRYYWKSVNI
                     DGLGGGVVEALKEHAEAAPSDHSTIDVWFQGGAMDRVGADESAFGKRSAIMLGIEANW
                     EDQGDDAANIGWARDCYSDLGRFSGGGVYLNFPGFLEGGQGLMRNAFGGNYERLVALK
                     NHYDPTNLFRLNQNIEPTT"
     gene            complement(2484..>2868)
                     /locus_tag="AC16CM_07365"
     CDS             complement(2484..>2868)
                     /locus_tag="AC16CM_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139685.1"
                     /translation="AQDAEALAALSERYPAFQKAYDEGKVTHHLLIARSDDLPREDAF
                     ELVAASAQRCRALGYQYMVTEATNQWTGAAFEALGGVRVHFAPFQVRPVVPESDGPLE
                     NVTTSPNGFLSDKDSGGMFYVVRLA"
BASE COUNT          483 a          968 c          854 g          563 t
ORIGIN      
        1 gctccacgat gccgccgacg gtcatggcac cgagccattc gcgcacgtag cgttcgctca
       61 ggtcggccgc atcggcgatc tccccgctcg tcgaggggga aagacccgac atcgcgtcga
      121 agaggcctgt gcgatgtccg atgctgatca tgaccgctat ggctccctcg ttgagcgtcc
      181 cgatcatacg tcccgcgaag gcttcggccc tttgctggtc gagttctctc gtgtccatgt
      241 catctctcct tttccgcccc gtggttcagg tcgtgggctc tatgttctgg ttgaggcgga
      301 acaggttggt aggatcgtag tggttcttga gggcgaccag acgctcgtag ttcccgccga
      361 aggcgttgcg catcaggccc tggccacctt cgaggaagcc ggggaagttg aggtacaccc
      421 cgcctccgga aaagcgccca aggtccgagt agcaatccct ggcccacccg atgttcgccg
      481 catcgtcccc ctgatcctcc cagttcgcct cgatgccgag catgatggcg ctcctcttac
      541 cgaaagcgct ctcgtctgcc ccgacccggt ccatggcccc gccctggaac cacacgtcta
      601 tggtggagtg atcggagggt gcggcctcgg cgtgttcctt caaagcttct accacgccgc
      661 cgccaagccc gtcgatgttc accgacttcc agtagtagcg ccacccgtcc gggtagtctt
      721 cgtccaggag tttctggacc tcggtgtagg gcatcgggcc actgaattcc gcgatcgggt
      781 cgccgagcac gcgcagtggc tcaagtacct ccgcaccctc ctcgacggga ccgtcgtaca
      841 cggccagggt tgccacgaac tgctcgccgg gccactcagc ggggaagtca tacacgcgtg
      901 ggacccggcc aaggaagcta agcgggctta ccccattggg cgcgccagcc acgtacgcct
      961 cgatggagcg cagcacctct tcggaacgat cccctgggta gaacacgaag gcgcacatta
     1021 cctcgggtcc cacgggatga agccggtact cgaacgaggt gatcacaccg aagttgccgc
     1081 caccgccgcg aacggcccag aacagatccg cgttctcctc cttgctcgcc gtcagcacgc
     1141 gtccgtcggc ggtaaccagc tccacggaga ggaggttgtc ggacgagagg ccgtgcttgc
     1201 gcctcaggtg gcccacgccg ccgcccagcg tgagtccggc gataccggtc tcggagacga
     1261 cgcccatggg cgtcgctagc ccgtgcttct gcgtctccgc gtcgagctcg cctatgatga
     1321 cgccagcccc ggcgcgtacg gtacgcgcgg cggggtctac ctcgatatcc ttcatggcag
     1381 agaggtcaag gaccagtcct ccctcggcgc tggccgtgcc ggcgacgttg tggccaccgc
     1441 cgcgcaccgc caggagcagg ccgttctcgc gggcgaagct tacgaccgcg accacgtcct
     1501 cgacgttggt gctgcgggct atgagcgcgg ggcgccggtc gatcacgccg ttccagagcc
     1561 ttcgagcctc ctcgtaaccg gcgtctacgg gacgaaacag gtcgccctct agcccggatg
     1621 caagcctctc cacctcagcc ccatggagcg aggattcttc tccccctacc gtcatgatct
     1681 ttactcccac caccatctcc tctctccgcg tcgtcggccc cgatttcggt gccttgctac
     1741 ccctcgcctg caaagtagct tgggacttcg gccgtagagc gacggccgtg cctagtacct
     1801 gcacggggtg aggaccgttc ccttagcgcc agtaggccac gggcgtgccg ccttcggtcg
     1861 tggtggtacc cgccggtgcc cttcccattt ctagtctctc ttctcatagc gcaggcctcc
     1921 tgactgttgg ttctcatcca gaacgtgcgg atacgaggtc cctgtctgta ttcacctcag
     1981 cattccgccg cctgtcgtct tcgaagggac cacgtgaaca ctcgacttgc cagcgcgctc
     2041 gcggttcccg agtttatcct ggagaaggcg cgaaggcgcc ggaccagtcg gtctttctct
     2101 atctgccgcg tcacgtcctc acggtgctgc ctggaaaggc cgatgtcgtt caggtcgtac
     2161 attccgggtt ccccactttc ctttgctttc tacgggtaca agtaaaacgc cggacgccga
     2221 tgcggacatc gggcgtaaga acgatcatcg gcacggactt ccggcggcat ggccacgtag
     2281 gactaaagtc ttatagtgag tgaagatcgc aaagatcggc gatcgattct agaaaaggcc
     2341 ccgagccaga acacccaact cgggagaaga tcccgtttca ttcagggctc tggcccagga
     2401 tcgctggtcg attcatcgca ccgttttcac gggtgctgga tgggacgaag ccacgtcctc
     2461 cgtcccatcg gtccgcgccg cctctaggcg agacgaacga cgtagaacat gcccccgctg
     2521 tccttgtcag agagaaaccc attcgggctc gtggtgacgt tttccagcgg tccgtcgctc
     2581 tctggaacga cgggacgaac ctggaatggg gcgaaatgta cgcgcacccc gccaagggcc
     2641 tcgaaggctg cccccgtcca ctggttggtc gcctcggtga ccatgtactg gtagcccaga
     2701 gcccggcacc tctgtgcgct ggccgccacg agctcgaaag catcttccct gggaagatcg
     2761 tcggaacggg cgatcagaag atggtgcgtg actttgcctt cgtcgtatgc cttctgaaac
     2821 gccggatacc gttctgagag tgccgcgagc gcctccgcgt cctgggcg
//
LOCUS       DBNGVD010000271         6231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2065356, whole genome shotgun sequence.
ACCESSION   DBNGVD010000271 DBNGVD010000000
VERSION     DBNGVD010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2065356"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16CM_07370"
     CDS             complement(<1..484)
                     /locus_tag="AC16CM_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3139686.1"
                     /translation="MERLGAKVRLDPINLGARNGDPLYRQVERRIEDLLLRGLYKAGD
                     RIPPEAELVSSLGVSRVTVRAGVARLVQRGVLERRQGSGTFLLRPPEGTRLASGLERL
                     ETYTVHARRLGLKLASADLTIEASGATAEEAAALDVGEGTPLAKVSRVLLIEGEPTAW
                     M"
     gene            675..2135
                     /gene="eat"
                     /locus_tag="AC16CM_07375"
     CDS             675..2135
                     /gene="eat"
                     /locus_tag="AC16CM_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HIR3139687.1"
                     /translation="MSEQERRTSGGAGYEDVGSDYLEQRQLQRGAAGWVLLAGLGVAY
                     VISGDFAGWHFGLGVGGWGGLLVATVLMATMYTAMVFSLAELSSTIPTAGGGYGFARR
                     AMGPLGGFMTGTAILIEYSIAPAAIATFIGGYVETLTGIGGWVVYLACYLVFVGIHIY
                     GVGEALKLMFAITVVAVVALAAFIIGMIPQFEVANLFTIDPTNAAGASSFLPFGVVGV
                     WAAFPYAIWFFLAIEGVPLAAEETRDPSTDMPKGLIGGMSALLLFAVCVLLFGPGGAG
                     AAAFTSDEMLAAPSVLPLALTEAYGGSNALVTFVNIAGLAGLVASFFSIIFAYSRQTF
                     ALSRAGYLPRVLSVTTGRRTPWVALIVPGIIGFLLALTGDGATLINIAVFGATISYAL
                     MMLSHIVLRRREPDLERPYRTPGGTVTSGIALVLAVLAVIATFIVDVRAAFITLGVYA
                     LFLAYFGLYSRNHLVAQAPEEEFATIEQAESELAGS"
     gene            2175..3512
                     /locus_tag="AC16CM_07380"
     CDS             2175..3512
                     /locus_tag="AC16CM_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3139688.1"
                     /translation="MDLDTLRSEVEAGRIDTVLLAMTDMQGRLQGKRLTASHFLQEVV
                     EHDAEGCNYLLAVDVEMNTVEGYAMSSWERGYGDFVFKPDMETLRRVPWQDGTALVTC
                     DLVWEDGSPVVASPRQVLKRQLDRLSERGLEAFVGTELEFLVFMDTYEEAWRRGYKDL
                     EPANYYNVDYSLLGTARIEPLLRRIRNSMAAAGLFVEDAKGECNLGQHEINFRFDRAL
                     ATADGHSIYKNGAKEIAAQENHSISFMPKFDEREGNSCHIHLSLRGEDGSAIFPDGDG
                     FSETFEHFVAGQISCLREFMLFFAPNINSYKRYAKGSFAPTAVAWGKDNRTCSLRVVG
                     HGNSLRVENRIPGGDVNPYLAVSAMIAAGLYGIENKLPLEDEFAGNAYETDKPHVPTN
                     LREARELFAGSEAARSAFGDEVVEHYLNMARVEIEAFEAAVTDWERYRNFERL"
     gene            3668..5029
                     /locus_tag="AC16CM_07385"
     CDS             3668..5029
                     /locus_tag="AC16CM_07385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3139689.1"
                     /translation="MLDIINPATEQVLESLTPATEDDADRAIARAREAFPAWRDVEPA
                     DRARLLRRLADALESERENLAQLESKNTGKPIGDSRGEMGMVAETFHYYAGMPERLLG
                     DTIPVSGGVDVTFREPLGVVGLIVPWNFPLTIASWKMAPALAAGNTLVLKPAELTPLT
                     AMEFESIAREAGIPDGVVNVLVGPGSKVGRRLVEHPDVAKVAFTGSTEVGRGIMAGAA
                     GTIKRVTLELGGKSANVVFADADLEKAAASAPAAVFGNAGQDCCARSRILVQRGVFDE
                     FLSLMEPAVTGIRVGDPFDEATAMGPLISADHREKVASYVTDDAPVAIRGEAPGGAGF
                     WFPPTVLAPVDNRDRAAEEEIFGPVVAVIPFEDEEDAVRLANDTVYGLSGSLWTGNIA
                     RAMRLARLIETGVLSINSNTSVRVATPFGGMKQSGVGRELGPHALDYYTEVKNVFVST
                     EED"
     gene            5031..5798
                     /locus_tag="AC16CM_07390"
     CDS             5031..5798
                     /locus_tag="AC16CM_07390"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIR3139690.1"
                     /translation="MPGRLSGKVAVVTGGAGGIGRETARRFAEEDARVCVVDLADEPG
                     GEVAEEIGGLYVNADVTDADEVQNVYAETARRFGGVDVLFNNAGISPPDDNSILDTDL
                     DAWQRVQDVNLKSVYLCCKYGIPHLLERGGGSIINTASFVAVMGAATSQISYTASKGG
                     VLALSRELGVEFARRGIRVNALCPGPVNTPLLQELFAKDPEKAQRRLVHLPMGRFAEA
                     TEIANAVLFLASDEASYVNASTFLVDGGLSGAYVTPE"
     gene            5862..>6231
                     /locus_tag="AC16CM_07395"
     CDS             5862..>6231
                     /locus_tag="AC16CM_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3139691.1"
                     /translation="MLEVEGIYCKLEYLNPSGSVKARIAKYMVERAEEEGLLKEGDTI
                     VEATSGNTGNALSMVAAVKGYRMLVVMPEGMSGERVAISRAFGADVLEVGHFHVNEAL
                     AKAKELGQEPGYFCPSQFESE"
BASE COUNT         1068 a         2046 c         2069 g         1048 t
ORIGIN      
        1 ccatccacgc ggtcggctct ccctctatga gcaggacgcg tgagaccttt gccagcgggg
       61 taccttcgcc gacgtccagc gcggcggcct cctcggccgt agcccccgag gcttcgatgg
      121 tcagatccgc gctcgccagc ttcaacccca ggcgccgcgc gtgaacggtg tacgtctcca
      181 gccgctcaag gcccgacgcg agccgcgttc cctccggcgg acgcaggagg aacgtaccgc
      241 tcccctggcg gcgctcgagc acgccccgct gcacgagtcg ggccaccccg gcccttaccg
      301 tgacccgcga cacccccagc gaagacacca gctcggcctc agggggaatg cggtccccgg
      361 ccttgtacag cccgcgcagc agaaggtcct ctatccgtcg ctcgacctgc cgatagaggg
      421 gatctccgtt tcgcgctccc aggtttatgg gatccagcct gaccttcgcc cccagacgtt
      481 ccaacgcaag agaatcctac ccgacggcgc gtggagcgca aggagatcgg gtgcccgcct
      541 cttgtatgac aaattggtat taactatgat gtgattgtag ccagacggcc gaggtgggcg
      601 gctgtccgtg actgttagcc tgggttgata ctgtcggcgg tgagtcggag cggacttcgg
      661 ggaagggagt tggggtgagc gagcaggaac ggcggacgtc gggcggtgcc ggctatgagg
      721 acgtggggtc cgattatctg gagcagaggc agctccagag gggggccgca ggctgggtgc
      781 tgctggccgg actcggtgtg gcctacgtta tctcgggcga ctttgcgggc tggcacttcg
      841 ggctcggcgt cggcgggtgg ggagggttgc tcgtcgccac ggtgctcatg gccacgatgt
      901 acacggccat ggtcttctcg ttggccgagt tgtcttccac catccccacg gcgggcgggg
      961 gctacgggtt cgcgcggcgg gccatggggc cgttgggcgg gttcatgacg gggacggcga
     1021 tcctgatcga gtactccata gcccccgcgg ccatcgccac gtttatcggg gggtacgtgg
     1081 agacgctcac cggcatcggc gggtgggtgg tgtacctggc gtgctacctg gttttcgtcg
     1141 gcatccacat ttatggtgtg ggggaggcgc tcaaactcat gttcgccata acggtcgtgg
     1201 ccgtcgtggc gctcgcggcg ttcatcatcg gcatgatccc gcagttcgag gtggcgaacc
     1261 tgttcaccat agatccgacg aacgcggccg gcgcgagctc gttcctgccg ttcggggtgg
     1321 tcggcgtctg ggcggcgttc ccttacgcga tctggttctt cctcgccatc gagggcgtgc
     1381 ccctcgccgc cgaggagacg cgggacccat cgacggacat gccgaagggg ctcatcgggg
     1441 gcatgtcggc gctcttgctc ttcgcggttt gtgtgctcct cttcggcccg ggaggggctg
     1501 gtgcggcggc gttcaccagc gacgagatgc tcgcagcgcc ttccgtgctg ccccttgccc
     1561 tcaccgaggc ctacgggggt agcaacgcgc tcgtgacctt cgtcaacatc gcggggctgg
     1621 ccggactggt cgccagcttc ttctccataa tcttcgccta ctcgcgccag acgttcgccc
     1681 tctccagggc cgggtacctg cccagggttc tatcggtcac caccggcagg agaacgccgt
     1741 gggtggccct gatagtgcct ggcatcatcg ggttcctcct cgcgctcacc ggtgacgggg
     1801 cgaccctcat aaacatcgcc gtcttcggcg cgaccatctc ctacgccctc atgatgctct
     1861 cgcacatcgt cttgaggagg cgtgagcccg acctggagcg gccgtaccgc acgccggggg
     1921 gtacggttac ctcgggcata gcgttggtcc tggcggttct ggctgtgatc gcgacgttca
     1981 tcgtggacgt gagggcggcg tttattacgc tcggcgtcta cgcgctcttc cttgcatatt
     2041 tcggcctcta cagccgcaac cacctcgttg cccaggcgcc ggaggaagag ttcgccacca
     2101 tagagcaggc cgaatcagag ttggccggaa gctagagagg agaactattg gacaccacgc
     2161 aggcggaggg acgtatggac ctcgacaccc tacgctccga ggtggaggct gggcgcatag
     2221 acacggtcct cctcgcgatg acggacatgc agggccgcct gcagggcaag cgcctcacgg
     2281 cctcccactt tcttcaggag gtggtcgagc acgacgcgga gggttgtaac tacctgctag
     2341 ccgtggacgt ggagatgaac accgtagagg gctacgccat gtcatcctgg gagcgcggct
     2401 acggcgactt cgtcttcaag ccggacatgg agaccctcag gcgcgtcccc tggcaggacg
     2461 gaacggcgct cgtgacctgc gatctcgtct gggaggacgg ttcaccggtc gtggcctcgc
     2521 cccggcaggt cttgaagcga cagctcgacc ggctctccga gcgggggcta gaggccttcg
     2581 tcgggaccga gctcgagttc ctcgtcttca tggacaccta cgaggaggcc tggcgcaggg
     2641 gttacaagga cctggagccc gccaactact acaacgtgga ctactcgttg ctcgggaccg
     2701 cgcgcatcga gcccttgctc cggcgcatcc gcaacagcat ggcggcggcg ggtctcttcg
     2761 tcgaggatgc gaagggcgaa tgcaacctcg ggcaacacga gatcaacttc cggttcgaca
     2821 gggcgctggc cacagccgac gggcactcca tctacaagaa cggcgcgaag gagatcgccg
     2881 cccaggagaa ccactccatc tccttcatgc ccaagttcga cgagcgcgag ggaaactcct
     2941 gccacatcca tctctccctg cgcggcgagg acgggagcgc catcttcccc gacggcgacg
     3001 ggttctcgga gaccttcgag cacttcgtag cgggccagat ctcctgcctg cgcgagttca
     3061 tgctgttctt cgccccgaac ataaactcgt acaagcgcta cgccaaagga tccttcgccc
     3121 cgaccgcggt ggcgtggggc aaggacaacc gcacctgctc tctgcgcgtc gtcggacatg
     3181 gcaactcctt gcgggtcgag aaccgtatcc ccggcggtga tgtcaaccct tacctggccg
     3241 tctcggccat gatcgccgcc gggctctacg gcatcgagaa caaactcccc ctggaggacg
     3301 agttcgccgg caacgcctac gagacggaca agccccacgt cccgaccaac ctgcgcgaag
     3361 cccgggaact attcgcgggc agcgaggcgg ccagatccgc gttcggggac gaggtcgtcg
     3421 agcattacct gaacatggcg cgcgtggaga tagaggcctt cgaggccgcc gtcaccgact
     3481 gggagcgcta caggaacttc gaacgcctgt gagggtgccg accgggtcgg acttccctgc
     3541 agcctccccg tgacggccag cccagaagat gatacggtcg aggtgcctga atacccccgc
     3601 ctgccctttc acagaggcag gagagaataa ggaccccaca aaagccgtcg taccggaggt
     3661 ttcctagttg ctcgacataa tcaacccggc cacggaacaa gtactggaga gccttacccc
     3721 cgccacagaa gacgatgccg atagggcgat cgcccgcgcc agagaggcgt ttccggcgtg
     3781 gagggacgtg gagccagccg accgcgcgcg actgctccgc cgcctcgccg acgccctcga
     3841 gtcggagcgg gagaacctgg cgcagctcga gtcgaagaac acgggaaagc cgatcggcga
     3901 ctcccgcggc gagatgggga tggtggccga gaccttccac tactacgccg gcatgcccga
     3961 gcgcctgctc ggagatacca tccccgtctc tggcggggtg gacgtcacgt tccgcgaacc
     4021 acttggcgtg gtcggtctca tcgtgccgtg gaacttcccg ctcaccatcg cgagctggaa
     4081 gatggcgccg gcgctcgcgg cgggcaacac gctcgtcctg aagcccgccg agctcacgcc
     4141 tcttaccgcc atggagttcg agagcatagc ccgcgaggcc ggcatcccgg acggcgtggt
     4201 gaacgttctc gtggggcccg ggagcaaggt cggccggcgc ctcgtcgagc acccagacgt
     4261 cgccaaggtc gccttcacgg gctccacgga ggtcggcagg ggcatcatgg ccggggccgc
     4321 gggaaccatc aagcgcgtca cgctggagct cgggggcaag tcggcaaacg tcgtcttcgc
     4381 cgacgccgac cttgagaagg ccgccgcctc cgcgccagcc gccgtcttcg gcaacgccgg
     4441 tcaggactgc tgcgcccgct cacgcatcct cgtgcagagg ggcgtcttcg acgagttcct
     4501 ctccctcatg gaacccgccg tcacagggat aagggtcggt gaccccttcg acgaggcaac
     4561 ggccatggga cccctcatct ccgccgacca ccgcgagaag gtggcttcgt acgtgacgga
     4621 tgatgcgccc gtggcgatcc ggggcgaggc accgggtggc gccggcttct ggtttccgcc
     4681 aactgttctg gcgcccgtgg acaacaggga ccgggcggcg gaggaggaga tcttcggccc
     4741 cgtggtggcg gtcataccct tcgaggacga ggaggacgcc gtccgcctcg ccaacgatac
     4801 ggtctacggc ctctccggct cgctgtggac ggggaacatt gccagggcca tgcgcctggc
     4861 acgcctcatc gagaccggcg tgctgtccat aaactccaac acctccgtca gggtcgcgac
     4921 gcccttcggc gggatgaagc agtcgggcgt gggacgcgag ctcggcccgc acgccctcga
     4981 ctactacaca gaggtcaaga acgtcttcgt gtccacggag gaggattagc atgccaggac
     5041 gcctgagcgg caaggttgcc gtcgtgaccg gtggcgccgg cggcataggc cgcgagacgg
     5101 ccaggcgctt cgcggaagaa gacgccaggg tctgcgtcgt cgacctcgcc gacgaaccgg
     5161 gaggcgaggt ggcggaagag atcgggggcc tctacgtgaa cgccgatgtg accgacgccg
     5221 acgaggtgca gaacgtgtac gctgagacgg cccggcgttt cgggggcgtc gacgtactct
     5281 tcaacaacgc cgggatctca ccccccgacg acaactccat cctggacacc gacctagacg
     5341 cctggcagcg ggtgcaggac gtgaacctca agagtgtcta cctgtgctgc aagtacggca
     5401 tcccgcacct gctcgagcgg ggcggtggtt ccatcatcaa cacggcctcc ttcgtggccg
     5461 ttatgggggc cgccacctcc cagatctcct acaccgcctc aaaaggcggc gtgctggcct
     5521 tgagccgcga gctcggggtg gagttcgccc ggagggggat acgcgtgaac gcgctctgcc
     5581 ccggtccggt gaacacgccc ctgttgcagg agctcttcgc caaggatcca gagaaagcgc
     5641 agcgcaggct cgtgcacctg ccgatgggcc ggtttgcgga ggccacggag atcgccaacg
     5701 ccgtcctctt cctggcttcc gacgaggcct cctacgtcaa cgcctcgacc ttcctggttg
     5761 acggcggcct ctccggggca tacgttacac cggagtagga ggtggtaggc gtgaacacag
     5821 gactatccaa caacatcctc gacgccatag ggaacacgcc gatgctcgag gtggagggca
     5881 tctactgcaa gctcgagtat ctgaacccgt ccggctccgt aaaggcccgc atagccaagt
     5941 acatggtgga gcgtgccgag gaggaggggc tcctgaaaga gggcgacacc atcgtggagg
     6001 ccacgtccgg caacacgggc aacgccctct ccatggtcgc cgcggtcaaa ggctaccgga
     6061 tgctcgtcgt gatgcccgag ggcatgagcg gggagcgggt cgcaatcagc cgcgccttcg
     6121 gggccgacgt gctcgaggtc gggcacttcc acgtcaacga ggccctcgcc aaagccaagg
     6181 aactcggcca ggagcccggc tacttctgcc cctcccagtt cgagagcgag t
//
LOCUS       DBNGVD010000272         4895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_792036, whole genome shotgun sequence.
ACCESSION   DBNGVD010000272 DBNGVD010000000
VERSION     DBNGVD010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_792036"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="AC16CM_07400"
     CDS             <1..385
                     /locus_tag="AC16CM_07400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139692.1"
                     /translation="IETEGQLIGATVLSNDENLPSRSATFGIIIGDRAYLDQGYGTEA
                     STLLLDYAFAVLGYHKVNLDLFEYNARARAMYERLGFVLEGRRRENHWSRGRFWDDIL
                     MGVTAEEWWAEHGRPQPPELGSARR"
     gene            382..1110
                     /locus_tag="AC16CM_07405"
     CDS             382..1110
                     /locus_tag="AC16CM_07405"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3139693.1"
                     /translation="MSSDAPFTVFPAIDLRGGKCVRLKQGDFGRSKEYDADPVQRARE
                     WERQGAEAIHVVDLDGAEQGSPVQLELVERMAGAVDVPLQVGGGIRRLNDLRAARASG
                     AERVVIGTGAVGDRRLRLAAVEEAGSSLVVAVDARDGIVATHGWRRSSGIPVLDLAEE
                     LAADGVASVLFTDVARDGMDAGAALEATAGVARAIPTVASGGVRGVEDVAALSEFPGV
                     VGVVVGTALYEGQVTLAQLMAARR"
     gene            complement(1153..1461)
                     /locus_tag="AC16CM_07410"
     CDS             complement(1153..1461)
                     /locus_tag="AC16CM_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139694.1"
                     /translation="MPTLDCTVRRTGDYESRTEEYELVYSVGASPSRSLNIKYQAARA
                     YEINAALDAAVETAREAIADDDAGQRGPTHEALAKFSWRAVQAAKYEEGEPAASSLEY
                     "
     gene            1530..3497
                     /locus_tag="AC16CM_07415"
     CDS             1530..3497
                     /locus_tag="AC16CM_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3139695.1"
                     /translation="MNDFFETRKKKPRRRKLGRFLRGVRALFNLALILGVCAVIVVAC
                     LYLFVSKEFEGDLDRTYPELAETSYVYDVNGQKVGEFPVAESRETVGYEGLGENLPMA
                     VVALEDRRFYDHWGFDPEGLARAAWTDLRSWQVEEGGSTLTEQLMKNLYVDQDERFET
                     SFRRRFVQAALAFTYERKHSKDEILTAYLNTVYFGDGAYGAQTAAQTFFGKDAKDLSL
                     SEAATLAGFLHAPSSYVTWEGDVIVPQARERRDWVLRLMREQGMISRQELEQAQAAPL
                     DFAPSPPPQDTAYTPFLEKVRRDVEARLGPQALQRGGLRIQTTMDPELQHAAVETPAE
                     VLPYPSDPSAAVVTVEPQTGAIRALAGQEGEFNLALDARRQPGSSFKPIVLAAALRDD
                     ISPQSTYISRELNFYYQDEFYEVHNYDFVERGEISVSDAMAESDNTVFVQLAADVGLQ
                     NVVETAEDLGITSTVEPYPSTAIGGLGAGVSPLDMASAYSTFAGGGIHRDPYSIESIE
                     RNSYGKSETVYDHEIRGQRVLSGNEAAVETEVLRGVVEDGTATMFHDLDEEIGHPSAG
                     KTGTTDNFADAWYVGYTPRLCTAVWVGYPEGRESLVGVHGLAEPNGETFPMDIWSVYM
                     AQATEGDLSLDFPAADESGLDVVSGDYSSGF"
     gene            3840..4088
                     /locus_tag="AC16CM_07420"
     CDS             3840..4088
                     /locus_tag="AC16CM_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139696.1"
                     /translation="MTSGNIRMGPDPDRIMNALKKALRDDPKMRERPAEEVARELAGG
                     GYLDEEPDPVLVAELLGTMDPEGPGRETDELTEEGNPT"
     gene            4147..4596
                     /locus_tag="AC16CM_07425"
     CDS             4147..4596
                     /locus_tag="AC16CM_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139697.1"
                     /translation="MDLRGLYAGSFIDWGAVSASWNKRTVPSRLLLFAARRYLEEGTK
                     AEPERAEKLSQAPLPDEIKLAFASPPAPDAPPSRRWGEFVDAAIATELEMVSYGERPP
                     LLHELRAGLDQAATEAGPETDQGRWFLARRDALPGMDLPENPEYLPI"
BASE COUNT          862 a         1530 c         1646 g          857 t
ORIGIN      
        1 gatcgagacc gaaggccagc tgatcggggc gacggtgttg agcaacgacg agaaccttcc
       61 gagccgctcg gcgacgtttg gcatcatcat cggcgaccgg gcttacctcg accagggcta
      121 cggtaccgag gcgagcaccc tgctcctcga ctacgcattc gcggttctcg ggtaccacaa
      181 ggtgaacctc gacctcttcg agtacaacgc ccgcgcaagg gccatgtacg agaggctggg
      241 tttcgttctg gaagggcgcc gcagggagaa ccactggtcg cgcggccgct tctgggacga
      301 catcctcatg ggcgttaccg ccgaggaatg gtgggcagaa cacggcaggc cacaaccgcc
      361 cgaactcggg tccgcgcgtc gatgagcagc gacgccccct tcacggtctt cccggcgata
      421 gacctgcgcg gcggcaagtg cgtgcgcctc aaacagggcg acttcggccg cagcaaagag
      481 tacgatgcgg accccgttca acgagcccgc gagtgggaac gtcagggggc tgaagccatc
      541 cacgtcgttg acctcgacgg cgccgagcag ggtagcccag ttcagttgga gctcgtggag
      601 cgcatggccg gtgcggtcga tgttccgcta caggtgggag gcggcatcag gagactgaac
      661 gacctgcgcg ctgcccgggc gagcggtgcg gaacgcgtcg taatcggcac cggcgcggtc
      721 ggcgacagga ggctacgcct ggcggcggtc gaggaggccg gctcctcgct ggtcgtcgcg
      781 gtcgatgcga gggacgggat cgtggccacg cacggctggc gccggtcgag cggtatcccc
      841 gtcctcgacc tcgccgaaga gctggccgcc gacggcgtgg cctcagttct gttcacggac
      901 gtggcccgtg acggcatgga cgccggcgca gccctcgagg cgacggctgg tgtcgcgaga
      961 gcgatcccta ccgtcgccag cggcggcgtc cggggtgtcg aagacgtggc cgccctctcc
     1021 gagttccccg gcgtggtagg tgtcgtggtc ggtaccgcgc tctacgaggg acaggtcacg
     1081 ttagcgcagc taatggcggc gcgtcgctag gctcatccgc cgtagaggcg gttcgctaac
     1141 cgccctcatc gttcaatatt ccaggctaga ggccgcgggt tcgccctctt cgtatttggc
     1201 ggcctgcacg gcgcgccagg agaacttggc cagggcttca tgggtgggac ccctctggcc
     1261 cgcgtcgtcg tcggcgatgg cctccctcgc cgtctcgacg gccgcgtcga gggcggcgtt
     1321 gatctcgtag gcgcgggcgg cctggtactt gatgttcagg ctgcgggagg gcgaggcgcc
     1381 gacggagtag accagctcgt actcctccgt gcggctctcg tagtctccgg tgcgcctcac
     1441 cgtgcaatcc aaagttggca tgcctgcatc ctatcatctg cgcgcttccc gtccccgata
     1501 aggttggggt agactacggc aggagggccg tgaacgattt cttcgagacc agaaagaaga
     1561 agccaaggag gcgcaagctg gggcgcttct tgcgtggggt gcgcgccctc ttcaacctgg
     1621 ccctgatcct tggtgtgtgc gccgtgatcg tggtcgcatg cctctaccta ttcgtctcca
     1681 aggagttcga gggcgacctc gacagaacct accccgagct tgccgagacc tcctacgtat
     1741 acgacgtgaa cgggcagaag gtaggggagt ttcccgtcgc cgagagccgc gagaccgttg
     1801 gatacgaggg cctcggcgag aacctcccga tggccgtcgt cgccctcgag gacaggcggt
     1861 tctacgatca ctgggggttc gacccggagg ggctcgcccg cgcggcatgg acagacctga
     1921 ggtcctggca ggtcgaggag ggcggctcca cgctcacgga gcagcttatg aagaacctct
     1981 acgtggacca ggacgagcgc ttcgaaacct ccttccgccg acgcttcgtc caggccgccc
     2041 tggctttcac ctacgagcga aagcactcca aggacgagat cctcacggcc tacctgaaca
     2101 ccgtgtactt cggtgacggg gcttacgggg ctcagaccgc ggcacagacg ttcttcggaa
     2161 aggatgctaa agacctcagc ctctccgagg cggccacgct cgccggcttc ctgcacgcac
     2221 cctcgtccta cgttacctgg gagggtgacg ttatcgtgcc gcaggcccgg gagcgccgtg
     2281 actgggtact ccgcctgatg cgggaacagg gcatgatctc acgtcaggag ctcgaacagg
     2341 ctcaggccgc gcccctggac ttcgccccca gcccgccacc gcaggacacc gcctatacgc
     2401 ctttcctcga aaaggtcagg cgtgacgtgg aagcccggct tgggccgcag gccctgcagc
     2461 gcggaggact ccgcatccag accacgatgg accccgagct gcagcacgcg gccgtcgaga
     2521 cgcccgccga ggtgcttccc taccccagcg atccctccgc cgccgtggtt accgtggagc
     2581 cccagacggg ggcaataagg gccctggcgg gtcaggaagg cgagttcaac ctggccctcg
     2641 acgccagaag gcaaccaggc agctctttca agcccatcgt gctagccgcc gcattgcggg
     2701 atgacatctc tccccagagt acctacatct cgcgcgagtt gaacttctac taccaggacg
     2761 agttctacga ggtccacaac tacgacttcg tggagcgggg ggagatctcc gtctctgacg
     2821 cgatggccga gtcggacaac acggtcttcg tccagctcgc cgccgacgtg ggcctgcaga
     2881 acgtggtcga gacggccgag gacctcggaa taacgagcac ggtggagccc tacccgtcta
     2941 ccgcaatagg tggactgggg gcgggtgtta gcccgctcga tatggcctcg gcctactcca
     3001 cgttcgccgg cggtggcatc caccgcgatc cctactccat cgagagcatc gagaggaaca
     3061 gctacggtaa gtccgagacc gtctatgacc acgagatccg gggccagcgt gtgctgagcg
     3121 gcaacgaggc ggctgtcgag acggaggttc tgaggggtgt ggtcgaggac ggtaccgcta
     3181 ccatgttcca cgacctggac gaggagatag gtcatccctc ggcgggcaag accggaacca
     3241 cggacaactt cgccgacgcc tggtacgtcg gctacacgcc acggctctgt actgctgtgt
     3301 gggtcggata tccggagggc cgggaatcac tggtcggtgt gcacggcctg gcagaaccaa
     3361 acggcgagac tttccccatg gatatctggt cggtctacat ggcgcaggcc acggagggtg
     3421 atctctccct ggacttcccc gcggccgatg agagtggcct cgatgtggtc agcggcgact
     3481 actcgagcgg gttctagcgc tgcgcttcgc ctacgctccg gctaacgcct ccgctcgtca
     3541 gcacgctccg gcctgaaggc tgtcgcttgt cagcacggcg attgacgcct tctcggcatt
     3601 tcggggtatt gcggtgtgac gctgagcgtt ccgctccagt atcggggcat gaaccacgag
     3661 tgggttgtgg cacccccttg ttgatccttg cttccgtcat ccccgtggaa gcgtcaggtg
     3721 gagtacatgg ggtccatctc gggtctgctg gcggggtgtg cgatgcgtgc gcacggggta
     3781 cctctgaaag aggttgcgtt gttggagtgg gagatgttag acgaccagcg ggaggtggga
     3841 tgaccagcgg caacatcagg atgggccctg atccggacag aattatgaac gctctcaaga
     3901 aagctcttcg ggatgacccc aagatgcgcg agcggcccgc cgaggaggta gcccgcgagt
     3961 tggcgggggg cggatatctg gatgaggagc ccgatccggt gctcgtggcc gagttgctcg
     4021 ggaccatgga tccggaaggc cctggacgtg agaccgacga gctgaccgaa gaaggaaacc
     4081 cgacgtaggg tggatccgcg gcggacaacc gcggcctttc ctgctctgtt gcgttaggat
     4141 ggattcatgg atttgcgcgg gctctatgcc ggttctttca tagactgggg ggcggtttcc
     4201 gcctcgtgga acaagcgcac cgttccatcc cggttgctgc tctttgccgc ccgccgctac
     4261 ctcgaagaag gtaccaaggc ggagccggaa cgcgccgaaa agctctcaca agccccgcta
     4321 cccgacgaga taaagctggc cttcgcctcg ccgccagcac cggacgcgcc cccctcccgc
     4381 cgctggggag agttcgtcga cgcggcgatc gcgaccgaac tggagatggt ctcctacggc
     4441 gagaggccgc cgctcctgca cgagttgcga gcgggcctgg atcaggcggc aacagaggcg
     4501 ggacccgaga cagaccaagg acgctggttc ctggcccgcc gcgacgcact acctggcatg
     4561 gatcttcccg aaaacccgga gtatcttccg atctaggcgt caggcgtcac cagtactttg
     4621 tattgggtct tttggggcct gcttcgattg tgcgacgctc gggcgtagca gtccggtccg
     4681 gaacccgaaa ccggtagcct gtaacctgta gcctgtttac aatcacgcca tcgccgtaaa
     4741 cgggatggga taacgaggag aaaggtacac gatggagtcg ataccgagtg ctagctacag
     4801 ccttaccttg cgggtcgagt ttccgcacga ggcgggggct ctagggaaaa tcctgaccgt
     4861 cgttggggat gccgacgggc tcgtcggcgc ggtgg
//
LOCUS       DBNGVD010000273         5765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_64131, whole genome shotgun sequence.
ACCESSION   DBNGVD010000273 DBNGVD010000000
VERSION     DBNGVD010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_64131"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="AC16CM_07430"
     CDS             <1..1436
                     /locus_tag="AC16CM_07430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3139698.1"
                     /translation="DGIRPDVLRAAIRDGEAPHLGAIAERGEAVWDAVSVFPSITPAA
                     TAAIATGEAPARSGIVGHAWYDEHERRVVVYGAMTETVMASGPIRVFHNNVWRMNRDD
                     LHAATLFETLHDRGFDGACVNFPVRRGPHTHPIRMKTVEGYLKGGRYLGPSVEGPKEY
                     FMGDLFYSRDTGFSGRKANGGVLRSVGINDDYAARVGAMLLKDRAAPFNLVYFFKGDS
                     IAHHHGLEAQRRWLVTADEYVARIFEAGGGTDRVLDEYAVMALSDHGHAPLLPKNRYV
                     DLRDIPGQTVSSGVKARFGNGATLVLVPNGRSALVYLRDDVGLDPLLDGLVGRRGVDL
                     AAWIDGDWATVRRPGREVRFRPGVGPADPFGRSWELQGDPLALDIQVTDGVLRYGDYP
                     DALERLWGCLHSPRCGDVVLSAAPGYTFGEISGGYHSASDHGSLHASDSEVFVLASGV
                     PAPRRITDVAPTLMSHFGTATLAGERA"
     gene            1429..2172
                     /locus_tag="AC16CM_07435"
     CDS             1429..2172
                     /locus_tag="AC16CM_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139699.1"
                     /translation="MPDSSNGPSGTEERAGLRPRYRRAFGCVTWVYMGMLVFLLANFL
                     LVLPSRYLGSGALFGYLPPRLAFLGTALILPGMVLGAMLGARTFRTPVRRGTRVGAGV
                     GAMIGFSGFFALEWLATALGFAERDQAFRIVLFPDLGGTLAYYAFVPLTLAATALAVY
                     ALYSKNSDFGRRLRLAVLGAVLAVLAGLGVMATDFDFLGVLGALVSTLAAAIGGYVSG
                     AGYARAGGNDMIPPGASIRPRPTRPRKPR"
     gene            2377..4779
                     /locus_tag="AC16CM_07440"
     CDS             2377..4779
                     /locus_tag="AC16CM_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3139700.1"
                     /translation="MGLPTISGHPPGNLSSELTSFIGRERETAEVKRGLEAFRLLTLT
                     GPGGCGKTRVALAVGESLATRFDGGVWWVELAPVSDPALVPRAVASALGVHEQVGRSV
                     DDLLHDHLESRRLLLILDNCEHLIGACATLANTLLRACPSLRVLATSREAMGIAGENV
                     VLVPCLSLPDPMASRPWGDAVRLFVDRAATVAPGFALDERNSSALVRICERLDGLPLA
                     IELAAAKVKVLSAEQICTRLDDCFRLLGTGNRTLLPHHRTLRATMDWSHALLDREERI
                     LFRRLSVFSGGFTLEAAEHVCAWGDIGENEVLAWLSRLVDKSLVLAGEQGGEARYHLL
                     QTVREYAREQLHESGEAAHASRRHAAFILDLAERAESGLTGTWQGWWLARLESELDNL
                     RAAIRWSLEEEPEACLRLAGALWEFCYMRGHYGEGREWLEGALARAGDSSPASYARAL
                     AGAGILDLLQCEYDRAISLLEESLAIFRKLEDRSGVGSVLQSLGSIARERGRYAEAEN
                     LHSESLALQRELGNKEGVARSLVGLGFAAWLQGDTVLAGSLCSEALNLYRELQDAEGM
                     AWSLVNLGAAAQHAGNPGRATTLLEEGLVLSRETGYKEGIAWSLDQLGVVAQRRGDHE
                     RASALFRESFELHVDLGDRWRAASVLESWAGTLRRQRRPEPAARLLGAAGGLRDQIST
                     PVPPCERATHEDNVSALRTAMGEETFAAALARGRALKPARILADNGPVDVQPTSPTSL
                     TTREVEVLRLVAHGLNDPQVAEQLFISRRTVHAHLRSIYQKLGVTTRTAATRFALEND
                     LL"
     gene            5078..>5765
                     /locus_tag="AC16CM_07445"
     CDS             5078..>5765
                     /locus_tag="AC16CM_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139701.1"
                     /translation="MIETGTFARLRAYRMGLLVTVLGILVTVSLAGPADAKTLTVNST
                     QDFSDVYPGDGVCDTIAVQLLKPCTLRGAIQEANSLAGMDVIYFDIPGGGVRTIEPDS
                     QLPAATQRVTIDGYTQPGATKNTLVRGNNAVLRIELDGSSAPGALLPDGLLVSASDSI
                     VRGLVINNFSGEGVQFSGSDNRLEGNFIGTNASGLGTNVGPDDPGNHLSGVIIAQGTS
                     GNVIGGDTPEK"
BASE COUNT          956 a         1861 c         1950 g          998 t
ORIGIN      
        1 tcgatggtat aaggccggac gtgttgcggg cggccatccg cgacggggag gcgccgcatc
       61 tcggcgccat cgccgagagg ggggaggcgg tctgggatgc ggtctccgtg tttccaagca
      121 tcacgcctgc ggcgacggcc gcgatagcca ccggggaggc acccgccagg agtggcatcg
      181 tggggcacgc ctggtacgac gagcacgagc ggcgggtggt cgtctacggc gccatgactg
      241 agaccgtgat ggcgagcggc cctataaggg tgttccataa caacgtctgg cggatgaacc
      301 gcgacgatct gcacgccgcg accctgttcg agaccctgca tgaccgcggt tttgacggcg
      361 cgtgcgtcaa cttccccgta aggcgcgggc cgcacacgca cccaatcagg atgaagaccg
      421 tggaagggta tctgaaaggc ggacgctacc tcggaccctc ggtggagggc ccgaaggaat
      481 actttatggg ggacctcttc tacagccgcg acaccgggtt cagcgggcgc aaggccaacg
      541 gcggtgtcct ccgctccgta ggtatcaacg acgactacgc ggccagggtg ggggccatgc
      601 tcctcaagga cagggcagcg cccttcaacc tcgtctactt cttcaagggt gactccatag
      661 cgcaccacca cgggctcgag gcccagcggc gctggcttgt caccgccgac gagtacgtgg
      721 ccaggatctt cgaggccggc ggtggcaccg accgggtact cgacgagtac gctgtcatgg
      781 cgctctcgga ccacgggcac gcgccgctgc tcccgaagaa ccgctacgtg gacctgcgcg
      841 atatcccagg ccagaccgtc tcttccggcg taaaagcccg cttcggaaac ggcgccacct
      901 tagtcctggt tccgaacggg cgctctgctc tcgtgtacct ccgcgatgac gtcgggcttg
      961 acccgctcct cgacgggctc gtcgggcgtc gcggcgtgga cctcgcggcg tggatagacg
     1021 gagattgggc caccgttcgc aggccgggac gcgaggttcg cttccgtcct ggcgtgggac
     1081 ctgccgaccc gttcggacgg tcctgggaac ttcaggggga tccgctcgcc ctggacatcc
     1141 aggtcaccga tggtgttctg cgctacgggg attatcccga tgccctggag aggttgtggg
     1201 ggtgcctcca ctcgccgcgg tgcggggacg tggtgctctc ggcggcgccg ggatacacat
     1261 tcggggagat ctccggtggc taccactcgg ccagcgacca cggctcgctg cacgccagcg
     1321 actccgaggt cttcgttctg gcaagcggcg tgccagcccc gcgcaggatc acggacgtag
     1381 ccccgaccct gatgtcccac ttcgggaccg cgacgcttgc cggggaacgt gcctgatagc
     1441 agcaacggcc cctctggaac tgaggagcgc gcggggttac ggccacgcta caggcgggcg
     1501 ttcgggtgcg tcacatgggt ctacatgggg atgctcgtct ttctactggc caacttcctt
     1561 ctcgtcctcc cgtcgaggta cctgggaagc ggggccctct tcggctacct accgccaagg
     1621 ctggcatttc tcgggacggc cctgatcctg cccggcatgg tccttggcgc catgcttggg
     1681 gcacgcactt tccggacgcc ggtcaggcgc ggaacccgcg tgggggcggg tgtgggggcc
     1741 atgatcggct tcagcggctt cttcgcgctg gagtggcttg ccacagcgct cgggttcgcc
     1801 gagagggacc aggcattccg catagtcctc ttcccagacc tgggtggaac gcttgcctac
     1861 tacgccttcg tcccgctgac gctggccgcg accgcgctcg cggtctacgc cctctacagc
     1921 aagaactccg acttcggacg aaggctcagg ctggccgtcc tgggtgccgt gctggcagtg
     1981 ctcgcgggcc tcggcgtaat ggcgacggac ttcgatttcc tgggcgttct tggcgccctg
     2041 gtctcgacgc tggcggcggc catcggcggc tacgtgagcg gggccggcta cgccagggcc
     2101 ggtgggaacg acatgatccc accaggcgcc agcatccgac cccgaccgac ccgcccccgc
     2161 aagccccgct gacaaagcac cggtgacacc ctggcttcgt caggggatgg ccgattctgc
     2221 tgctattgcg cccgaaagct ctcccgtaca cacgtgttct tgcctggttt gatataaacc
     2281 cggtatgtag tgtgggttac gaccgagggg atcgtaggcc tggcgcgctc gtctcacaac
     2341 gcttcgcttc ggcaggatcc ggaaaatagc cgcgcggtgg gactgcctac catctcggga
     2401 catccgcctg gcaacctgtc gtcggagttg accagcttca tcgggcgcga gcgggagacg
     2461 gccgaggtga aacggggcct cgaggccttc cggctactca cgctgaccgg tccgggaggc
     2521 tgtggaaaaa cacgcgtggc cctggccgtc ggggagagcc ttgcgacacg attcgacggc
     2581 ggcgtgtggt gggtggagct tgcgccggtc tcggatcccg ccctcgtgcc gcgggccgtg
     2641 gcctccgcgc ttggcgtaca cgagcaggta ggacgttcgg tggacgatct gctccacgat
     2701 catctggagt cgaggaggct gctcctaatc ctggacaatt gcgagcacct gatcggggcc
     2761 tgcgccacgc tcgcgaacac gttgttgcgc gcctgcccca gcctgcgggt gcttgcaacc
     2821 agccgggagg ccatgggcat cgccggcgag aacgtcgtgc ttgtaccctg cctctccttg
     2881 cctgacccca tggcctcccg gccgtggggc gatgccgtca ggctctttgt cgacagggcc
     2941 gcgaccgtgg caccgggctt cgcgctcgac gagcgaaact cgtctgccct ggtgcggata
     3001 tgcgagaggt tggatggcct gccccttgcc atcgagctgg cggctgccaa ggtgaaggtg
     3061 ttgtcggcgg agcagatctg cacccgcctc gatgactgct tccggttgct tggaaccggc
     3121 aaccgtaccc tgcttcccca tcacagaacg ctccgggcga ccatggattg gagccacgca
     3181 ctgctcgacc gggaggaacg aattctgttc aggaggctct cggtgttttc tggtggcttt
     3241 acgctggagg ccgccgagca tgtctgtgcg tggggtgaca tcggggaaaa cgaggttctc
     3301 gcctggctct cccggctggt ggacaagtcg ctggtgctgg cgggggagca gggaggcgag
     3361 gcccgctacc acctcctgca gaccgtcagg gagtacgcgc gggagcagct tcatgagtcg
     3421 ggggaggccg cccacgcgag ccggcgtcac gcagccttca tcctggacct cgccgaacgc
     3481 gccgagtccg ggctcacggg tacgtggcag gggtggtggc tggcccgcct ggagtcagag
     3541 ctcgacaacc tgcgggcggc gattcggtgg tctcttgagg aggagcccga ggcatgcttg
     3601 cggctggcgg gtgccctgtg ggaattctgc tacatgcgcg gccactacgg ggagggacgc
     3661 gagtggttgg agggcgcgct cgcccgggcc ggcgattcgt cgccggcctc ctacgccagg
     3721 gcgctcgccg gcgccggaat ccttgatctt cttcagtgcg aatacgaccg ggccataagc
     3781 ctactcgagg agagcctggc catcttcagg aaactggaag acaggtcggg cgtcggctcg
     3841 gtactgcaga gcctggggag catcgcgcgg gagcggggcc gctacgccga ggcggagaac
     3901 ctccacagcg agagcctcgc cctgcagcgt gagctgggca acaaagaggg tgtcgcccgc
     3961 tccctcgtcg gcctgggttt tgcggcatgg ctgcagggag ataccgttct cgccgggtcg
     4021 ctgtgctctg aagccctgaa cctctaccgg gagctgcaag acgccgaggg catggcctgg
     4081 tcgctcgtca acctgggcgc tgcggcgcag cacgcgggca accccgggcg ggcgacgacg
     4141 cttctggagg agggcctcgt gctctcccgg gagaccgggt acaaggaggg catagcctgg
     4201 tcgcttgacc agctcggtgt cgtggcacag aggcgtggcg accacgagcg ggcgtcagcc
     4261 cttttcaggg agagcttcga gctgcacgtg gatctgggag acaggtggcg cgccgccagc
     4321 gtgctggagt catgggcagg gactctccgc aggcaaaggc gtcccgaacc ggcggcacga
     4381 ctcctcggcg cggccggggg cctgcgcgat cagatctcca cgcccgtccc accctgcgaa
     4441 cgcgccaccc acgaggacaa cgtgtctgcc ctccgcacgg ccatgggcga ggaaaccttc
     4501 gcggcggcgc tggcgcgcgg gcgggcgctg aaacccgcac gtatcctcgc cgacaacggg
     4561 ccagtagacg tccaaccaac ctcacccacc agcctgacca cccgggaggt cgaggtgctc
     4621 cggctcgtgg cccatggcct caacgatccg caggtcgccg agcaactctt catcagccgc
     4681 cgcaccgtac acgcccacct gcgctccatc taccagaaac tcggcgtgac caccagaacg
     4741 gcagccaccc gcttcgccct ggagaacgat ctactgtagc gttccagacc acgttgtagt
     4801 aaccccgcca gacgatctac agtggttctc ggagagatcg aacctgctca aagggtgcgt
     4861 tcagcaagca gcactcagct tatctgcttg tggcagatgg cttgtagctt gctttccaca
     4921 acgtcggtca tttcgcagac cgacctaggc aatattgccg atgtgcttta tgagacatac
     4981 gaatagcctg agacggacag gcgcacgttg gagattcccg tgaagggcgg actggtctgg
     5041 tcgtcggtcc gggacacaag ttaccgaggt gttgggcatg atcgagaccg gtacttttgc
     5101 gaggttgcgg gcgtacagga tggggcttct ggttacggtt cttgggatcc tggtaaccgt
     5161 cagcctggct ggacccgccg acgccaagac cctcaccgtc aactccacgc aggacttctc
     5221 ggacgtatac ccgggcgatg gcgtctgcga taccatcgcc gtccagctcc tcaagccctg
     5281 taccctgagg ggcgccatac aagaggccaa ctccctcgcc ggaatggatg tcatctattt
     5341 cgatatcccg ggaggagggg ttcggaccat agagccggac tcccagttgc ccgcggcgac
     5401 ccaacgggtc acgatagacg gctacaccca acccggcgcg acgaagaaca ccctggtccg
     5461 gggcaacaac gccgtgctca ggatcgagct cgacggcagc agtgctcccg gcgcgctgct
     5521 gccagacgga cttctggtca gtgcttcgga cagcattgta agggggctcg tgatcaacaa
     5581 cttctccgga gaaggggtgc agttcagcgg ctcggataac aggttggagg gcaactttat
     5641 aggcaccaac gcctccggcc tcggaaccaa cgtgggcccc gacgaccccg gcaaccacct
     5701 gagcggcgtg atcattgccc agggcacgtc cggcaacgtc atcggggggg acaccccgga
     5761 aaaac
//
LOCUS       DBNGVD010000274         7846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2433664, whole genome shotgun sequence.
ACCESSION   DBNGVD010000274 DBNGVD010000000
VERSION     DBNGVD010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2433664"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="aroC"
                     /locus_tag="AC16CM_07450"
     CDS             <1..778
                     /gene="aroC"
                     /locus_tag="AC16CM_07450"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3139702.1"
                     /translation="SSARETTARVAAGGVARKLLSEFGVEITSAVSRIGEVAYPKENA
                     ALEAASADESEVRCPDPEVTERMKAEIDAARYARDALGGEFVVVARGLPPGLGSYVDW
                     RDKLDARLAAAMMSINAIKGVEFGMGFELAARRSSEVQDEILRENGRLARNTNRLGGF
                     EGGMTNGEPVVVSAAMKPISTIAKALRTVDLATGEQTRAFKERADSCAVPAAAVIGES
                     MAAIVLAEAFLEKFGMDNMTDIRADYEAYGVRIAATASGT"
     gene            782..1276
                     /locus_tag="AC16CM_07455"
     CDS             782..1276
                     /locus_tag="AC16CM_07455"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3139703.1"
                     /translation="MEGSVAIVGYMGSGKSTLGRLLASATGLEFVDLDRAITRKAGRE
                     IPEIFAEYGEKRFRDLEQEALRESLEAASPRIVACGGGVVTRRENRALLQGVTTVFLR
                     EDVDVLYGRTRGPGRPLRAASRKEFEARYAERLPLYREVADMEVEIDGRSPSLVVEEV
                     LECL"
     gene            1267..2346
                     /gene="aroB"
                     /locus_tag="AC16CM_07460"
     CDS             1267..2346
                     /gene="aroB"
                     /locus_tag="AC16CM_07460"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3139704.1"
                     /translation="MSVETVEVPVARPYPVVVGAGLDLGAVVSELLQPGLCAVVTDAT
                     VGPLYAASVRDVLEAAGWRVAGEIEVPTGERSKSMDVYADTVGRLARLGLTRDGTVFA
                     LGGGVVGDLGGFVAATYMRGVDLVMLPTSLLAMVDSSVGGKVGVDLPEGKNLVGAFIR
                     PRLVAADLQWLGTLPEGEVSNGLAETIKMGLLSGGEYFEDLSLIPSARSGDLDALQRL
                     VLHSIRFKARVVAEDELEGGLRAILNYGHTIGHGLEAAADYGLPHGSAVAAGMVAAAT
                     LARDRYGRDLVALHENLLDAAGLPRRVSGVDTDRALEAMSRDKKRTAGNAEHRFVLLG
                     DIGQPVRNVPVSERQAREAIGVVLG"
     gene            2348..2767
                     /locus_tag="AC16CM_07465"
     CDS             2348..2767
                     /locus_tag="AC16CM_07465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3139705.1"
                     /translation="MFVLNGVNLGTLGTRRPEVYGTMTLDDIERFVSEAFPDVDLEFR
                     QTDFEGEMVGYIREAADHDGLIINAGAWTHYSYALHDALEAVDVPKVEVHLSNVHRRE
                     GWRRESVISPAVHAVIAGMGAQGYVAAVGYVLESSSP"
     gene            complement(2817..3797)
                     /locus_tag="AC16CM_07470"
     CDS             complement(2817..3797)
                     /locus_tag="AC16CM_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIR3139706.1"
                     /translation="MPSSELRRLVEAAHLYYINDFTQERVASLMGVSRSKVSRMLKEA
                     RARGLIEFRVRSSLITEPGLQKVLQRELGLKECLVLAAPESSDEAERVGALAGQYLQE
                     NIDDGNVVGVGWSSAVYSTVTNSGLQKRQDLVVAQLMGSVGNAIIELNGMYVTGRLAD
                     DLGASAHYIHAPMIVTDTAVRDGLLRDPYIRKTLEVARHADVILVGIGAVNENLGQYR
                     AGYLDDADLEDLWARGAVGEVIGTYISPEGQVVPLELNERIVGLDFRAMRKIPLRVGV
                     SWGARKALANIGAARSGLINVLVTDENTADEMATIINKGTNAKPGETTSL"
     gene            4007..5398
                     /locus_tag="AC16CM_07475"
     CDS             4007..5398
                     /locus_tag="AC16CM_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3139707.1"
                     /translation="MRRFARKDFLKLGGMLAAGAATAPLASACGGSGAASGGRLAGTS
                     LTVATVSNSQMEDMESLVGDFTSKTGIEVQFLFLPENDLRQRVTQDVAMRAGNFDIVT
                     IGSYDTPFWGRYEWTVPLDPFFEKMSATERREYDLEDLLAPIRTVLSNQGQLFALPFY
                     AESSMLFYRTDLFKEAGLQMPDRPTWEEVSGFAKQVHDPDDGVYGMVLRGLPGWGANM
                     APFGTFINTFGGRWFNENWEPQLETPEVKEAARVYSDLVTNYGQPGATSDNFPECQNL
                     FASGDGAMWYDATSTAGYLSDPETSEIADNVGFAYGPTGTTERGAHWLWSWALGIESS
                     SRNQEAAFEFLKWATSKEYIDLVGRELGWVRVPPGTRRSTYQDTKYKNNPWAEIEIQS
                     IETANPERPTRQPVPYTGIQYVGIPEFQQLGDEVGRSLASVLAGDMSIDDMTQLSQQQ
                     ALEVARQGQYLKS"
     gene            5545..6387
                     /locus_tag="AC16CM_07480"
     CDS             5545..6387
                     /locus_tag="AC16CM_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3139708.1"
                     /translation="MYPGLIVVAIITQIPFLLTLYLSMQSWNIIRPDLGRRFVGLGNY
                     TREIFSAEFGAIALQTLALTVMALVICCLVGLGLALLLNRDFFAKGLVQTLLVTPFFI
                     MPTVTGLIWKNELFSPNFGLVTWMAGLLGIEGFNPMADYPLLMVSTMVAWQWEPLFML
                     VLLAGLAAIPEEEKEAAQLDGCTRLGIFRYVELPHLLPYYRMVILLGSIFILQVFGEI
                     YVGTAGGPGTSSMNLQYLTYQTGLVGWQVGAASAIGVATLVLTLILLTGLLRTMNAVV
                     REGA"
     gene            6389..7234
                     /locus_tag="AC16CM_07485"
     CDS             6389..7234
                     /locus_tag="AC16CM_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3139709.1"
                     /translation="MSSVPASSKDRRRTPWVHYLQNTVLYLAVVVIFGPIFWLILTGF
                     KEDAAAYVLPPQIFFTPTFEQYASALQEFWSPFFNSLVIVGTSTVICLVLGIPAAFAL
                     SYFHSPRNESIVFWFITTKALPLAAVLTPLFVIFRSLRLLDTPWALIIVFTGMNTPIV
                     VWMMYQFMKDLPRGIMEAAQVDGVNLFQMLWRIVIPLTRAGIASTAMLCVIFSWNEFL
                     FSVSFTAIEWQPLSVAVAAQQTSRGQFWAQLSAFTTLAILVPVVVGWLTQKQLVRGLT
                     TGAVK"
     gene            7282..>7846
                     /locus_tag="AC16CM_07490"
     CDS             7282..>7846
                     /locus_tag="AC16CM_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR3139710.1"
                     /translation="MTKVSVLGSGVMASAMVFPLTDNGHEVNLVGTHLDREIIDSIQN
                     TGVHPDLDLELPESVRAFQLEDAEEAFEGAEIVMSGVNSFGVKWASEQLAGLLKPGMM
                     VIAVTKGLEADDEGNLSILPDVLASAVPEDLRAEVSWSAITGPAIAGEVAVKHDTCVV
                     FAGLDEGVLESLAETFRTDYYHVWTSTD"
BASE COUNT         1368 a         2467 c         2603 g         1408 t
ORIGIN      
        1 ctccagcgcc cgcgagacca ccgcgcgggt cgcggccggc ggcgtcgcca gaaagctcct
       61 ctccgagttc ggggtcgaga tcacgagcgc ggtctccagg atcggggagg tagcctaccc
      121 gaaggagaac gccgcgctcg aggccgcaag tgcagacgag tcggaggtca gatgcccgga
      181 ccccgaggtt acggagcgga tgaaggccga gatcgacgcc gcccgctacg cccgcgacgc
      241 cctgggaggc gagttcgtgg tcgttgcaag aggtcttccg cccgggctag gctcctacgt
      301 ggactggcgc gacaagcttg acgccaggct cgcggcggcc atgatgtcca taaacgccat
      361 caagggcgtg gagttcggga tgggcttcga gcttgcggcc cgccgctcga gcgaggtgca
      421 ggatgagatc ctgcgcgaga acggccgcct ggcacgcaac acgaaccgcc ttggcggctt
      481 cgaaggcggg atgaccaacg gggagcccgt ggtggtctcg gctgcgatga agcccatctc
      541 cactatagcc aaggcccttc gcacggtcga cctcgcaacg ggagagcaga cccgggcctt
      601 caaggagcgg gccgactcct gcgcggtgcc ggccgcggcc gtgatcgggg agtcaatggc
      661 agcgatcgtg ctggcagagg ctttcctgga gaagttcggc atggacaaca tgaccgacat
      721 ccgggccgac tacgaggcct acggagtgcg catcgccgcg accgcatcgg gaacctgagg
      781 cttggagggg tccgtggcca tcgtggggta tatggggagc gggaagtcca cgctgggtcg
      841 ccttctcgcc agcgctactg gcctcgagtt cgtggacctc gaccgggcca tcaccagaaa
      901 ggctggcagg gagataccgg agatctttgc cgagtacgga gagaagcggt tcagggatct
      961 ggagcaggaa gctcttcgcg agtcactgga agccgctagc ccccgcatcg tggcctgcgg
     1021 gggtggtgtg gtgacgcgcc gcgagaacag ggcccttctg caaggagtga ccacggtttt
     1081 cttgcgcgag gatgtggacg ttctgtacgg caggacccgg gggccagggc gtcccctgcg
     1141 cgccgccagc aggaaggagt tcgaggcgcg ctacgccgag cgcctccccc tctacaggga
     1201 agtcgccgac atggaggtcg agatcgacgg gagatccccc tccctggtgg tcgaggaggt
     1261 gctcgagtgt ctgtagagac cgtggaggtg cccgtcgccc gtccctaccc cgtggtggtc
     1321 ggggccgggc tcgacctcgg cgccgtggtc tccgaactcc tgcaaccggg cctctgcgcg
     1381 gtcgttacgg acgccacggt tggcccgctc tacgccgcca gcgtgcgcga tgtgctagaa
     1441 gcggccggct ggagggtggc gggggagatc gaggtcccca ccggcgagcg gtccaagagc
     1501 atggacgtct acgccgacac ggttggtagg ctcgcccgcc tggggttgac ccgcgatggc
     1561 accgtcttcg cgctcggtgg cggggtggta ggggatctcg gcggcttcgt cgccgccacc
     1621 tacatgcgcg gggttgacct cgtgatgctg ccgacctcgc tgcttgccat ggtggacagc
     1681 tcggtcgggg gcaaggtggg tgtcgacctc ccggagggca agaacctggt tggcgccttc
     1741 atccggccga ggctggtcgc agccgatctc cagtggctcg gcacgcttcc tgaaggcgag
     1801 gtctcaaacg gtctcgctga gactataaag atgggcctgc tctccggggg agagtacttc
     1861 gaggatctgt ccctcatccc atcggcccgc tcgggagatc tcgacgcgct gcagaggctc
     1921 gtcctgcact ctatccgctt caaagcgcgc gtcgtggcag aagacgagct ggaggggggc
     1981 ctgcgcgcga tcctcaatta cggccacacc atcggacacg gcctggaggc cgcggctgat
     2041 tacggcctac cccacgggtc ggccgtggcc gccggcatgg tcgccgccgc gaccctcgcc
     2101 agggaccggt acggccggga tctcgtcgcc ttacacgaaa acctgctaga tgcagccggc
     2161 cttccccgcc gggtgtcggg cgtcgacacg gatcgggcgc tcgaggccat gagcagggac
     2221 aagaagcgaa cggctggcaa cgccgaacac aggttcgtgc tgctagggga catcggccaa
     2281 cccgtaagga acgtgcccgt gagcgagcgg caggcgcgcg aggcgatcgg ggtcgtcctt
     2341 ggctagggtg ttcgtcctga acggcgtgaa cctcggtacc ctcggtacgc gtcgtcccga
     2401 ggtctacgga accatgaccc tcgacgacat agaaaggttc gtctcggaag cgttcccgga
     2461 cgtggacctg gaattcagac agacggattt cgaaggcgag atggtcgggt acatcaggga
     2521 ggcggccgat cacgacgggc tgatcatcaa cgccggcgcc tggacccact actcgtatgc
     2581 cctgcacgac gcgctcgagg ccgtggatgt gccgaaggtc gaggtccact tatcaaacgt
     2641 gcacaggcgc gaagggtggc ggcgtgaatc cgtcatctca cccgccgttc acgccgtgat
     2701 cgccggtatg ggagcccagg gttacgtggc cgcggtcgga tacgtcttgg aatcgtccag
     2761 cccgtaaagc acgccgcagg cgggtactcc agcaccggaa atcctgagca ccagggttag
     2821 agagaagtcg tctccccggg tttcgcgttg gttcccttgt ttatgatggt cgccatttcg
     2881 tcagccgtgt tctcgtcggt gacgaggacg ttgatcaggc ccgatcgcgc cgcgccgatg
     2941 ttggcgaggg ccttcctggc accccagctt actcctacgc ggagcgggat cttgcgcatc
     3001 gctctgaaat ccagtcccac gatgcgctcg ttgagttcta gtggcaccac ctgaccttct
     3061 ggggagatgt aggtgccgat gacttcgccg accgctcccc gcgcccataa gtcctcgagg
     3121 tcggcgtcgt cgaggtagcc ggcgcggtac tgccccaggt tctcgttgac ggccccgatc
     3181 cccacgagga tgacgtcggc gtgccgggcc acctcgaggg ttttgcggat gtaggggtcg
     3241 cgcaatagac cgtcgcgaac cgcggtgtcc gtcacgatca tcggcgcgtg tatgtagtgc
     3301 gcgctcgcac caaggtcgtc tgccagccgg ccggttacgt acatgccgtt gagctcgatg
     3361 atggcgttgc ccacgcttcc catgagctgc gccaccacga ggtcttgcct cttctgcagg
     3421 cccgagttgg tcaccgtgct gtacaccgcg ctgctccaac ccacacccac gacgttcccg
     3481 tcgtctatgt tttcctgcag atactgtccc gccagcgcgc cgaccctctc ggcctcgtcg
     3541 gaggactccg gggccgccag caccaggcac tccttcaagc ccagctctct ctgtagaacc
     3601 ttctgcaagc caggctcggt gatcagggat gaccggaccc gaaactcgat caaaccgcgc
     3661 gccctggcct cctttagcat gcgcgatacc ttggagcgcg atacccccat gagcgaggcg
     3721 accctctcct gcgtaaagtc attgatgtag tacaggtgcg cggcctcgac cagacgccgc
     3781 aactcactgc tgggcacttg tgtccccgaa tttcacaccg tatcccggcg ccatgttctc
     3841 gcgcgtactc ccgtgttccg cggtcccacc tgggagccat tattgcacgt ttgtgcagaa
     3901 ataatgcaga aaattgacaa ccgcgatcgg atgaaactag aatggcgagg gaccggagag
     3961 gaggatcgga ttggcgaagc aggggacgcg gccagtcggg caagatatgc gtaggtttgc
     4021 gcgcaaggac ttcctcaagc tggggggcat gctcgccgcg ggcgcggcaa ccgcgccgct
     4081 ggcctcggcg tgcggtgggt cgggcgcggc ctcgggcggc cggttggccg gcacgagcct
     4141 gaccgtggcg acggtcagca actcgcagat ggaggacatg gagagcctcg tcggggactt
     4201 taccagcaag accggcatcg aggtccaatt cctcttcctt ccggagaacg acctgcggca
     4261 gcgggtgacg caggatgtgg cgatgcgggc gggcaacttc gacatcgtca ccatcggcag
     4321 ctacgacacg ccgttctggg gccgctacga atggacggtg ccgctcgacc cgttcttcga
     4381 gaaaatgagc gcgacggaaa gacgggagta cgacctcgag gatctgctcg cgcccatccg
     4441 gacggtgctt tcgaaccagg ggcagctctt cgcgctcccg ttctacgccg agagctcgat
     4501 gctcttttac aggacggacc tcttcaagga ggctgggctg cagatgcccg accggcctac
     4561 gtgggaggag gtctccggct tcgcaaagca ggtgcacgat ccggacgacg gcgtctacgg
     4621 catggtcctg cggggtctgc ccggatgggg ggcgaacatg gcccccttcg ggacgtttat
     4681 caacaccttc ggtgggcgct ggttcaacga gaactgggag ccgcagttgg agaccccgga
     4741 ggtcaaggag gcggccagag tctattcgga tctggtgacg aactacggcc agccgggtgc
     4801 gacctcggac aacttccccg agtgccagaa cctcttcgcg tccggcgacg gggccatgtg
     4861 gtacgacgcg acctcgacgg ccggctacct gagcgacccc gagacctcgg agatagcgga
     4921 caatgtcggt ttcgcctacg ggccgacggg gaccaccgag cgcggggcgc actggctctg
     4981 gtcttgggcc ctggggatcg agtcctccag ccgcaaccag gaggccgcct tcgagtttct
     5041 caagtgggca acgagcaagg agtacatcga tctcgtgggc cgggagctcg gttgggtgcg
     5101 ggtgccgcct ggaacgcgcc ggtccaccta ccaggataca aagtacaaga acaacccgtg
     5161 ggccgagata gagatacagt ccatcgagac cgccaacccc gagaggccca cgcggcaacc
     5221 cgtcccctac acggggatcc agtacgtggg gatcccggag ttccaacagc tcggcgacga
     5281 ggtgggcagg agcctggcga gcgtcctcgc gggagatatg agcatcgacg atatgaccca
     5341 gctgtcccag cagcaggcgc tggaggtggc ccgccagggg cagtacctga agagctagcg
     5401 gccagggtcc cgacgcacgc agcgaggtaa gggaggttcg tagcggtgca agcaagcgac
     5461 agaatatccg aaccggcgac ggcccgggga gctccagggt cccgcagagc ccccgcctcg
     5521 ccccgtcggc agcgagcttc gctgttgtat ccgggcctga tcgtggttgc catcatcacg
     5581 cagataccct tcttgctaac cctgtacctg agcatgcaat cctggaacat cataagaccc
     5641 gatctcgggc gtcgctttgt ggggctcggc aactacacgc gcgagatctt ctcggcggag
     5701 ttcggcgcca tcgcgctgca gaccctcgcc cttacggtga tggccctggt gatctgctgt
     5761 ctcgtcgggc ttgggctggc gctgcttttg aacagggact tctttgccaa agggctcgtc
     5821 cagaccctgc tcgtcacgcc ctttttcatc atgccgaccg tgacgggcct catctggaaa
     5881 aacgagctgt tcagccccaa tttcggtctc gtcacctgga tggccggcct cctggggatc
     5941 gaaggcttca accctatggc ggattaccca ctgttgatgg tctcgacgat ggtcgcctgg
     6001 caatgggagc cgctattcat gctcgtgctg ctcgcggggc tcgcagccat cccggaggag
     6061 gaaaaggagg cggcccagct cgacgggtgc acgcggctcg ggatcttccg ctacgtcgag
     6121 ctccctcatc tgctgccgta ctaccggatg gtcatactac tcggtagcat ctttatcctg
     6181 caggtgttcg gcgagatcta cgtgggcacc gcgggcggtc caggaacgag ctcgatgaac
     6241 ctgcagtact tgacgtacca gacgggcctc gtcggctggc aggtcggggc ggcctcagcc
     6301 attggggtcg ccacgctggt gctgactctc attctgctga cgggcctgct gaggacgatg
     6361 aacgccgtgg taagggaggg agcgtaacgt gagcagcgta ccggcctctt cgaaggacag
     6421 acggcggacc ccctgggtcc actacctgca aaacacggtt ctctacctgg cggtcgtcgt
     6481 gatcttcggc ccgatcttct ggctgatcct caccggcttc aaggaggatg ccgccgccta
     6541 tgtgctgccg ccccagatct tcttcacccc gaccttcgag cagtacgcgt cggcgctgca
     6601 agagttctgg tcgccgtttt tcaactcgct ggttatagtc ggcacctcga ccgtaatctg
     6661 cctggtgctc ggcatcccgg cggccttcgc cctgagttac ttccacagcc cgcgcaacga
     6721 gagcatcgtg ttctggttta ttacgaccaa ggcgctgccg ctggccgcgg tactgacgcc
     6781 gctgttcgtc atattccgct cgctccgctt gctggacaca ccgtgggcgc tcataatcgt
     6841 gttcacgggg atgaacacac caatagtggt gtggatgatg taccagttca tgaaggacct
     6901 gccgcggggc atcatggagg cggcgcaggt cgacggggtc aacctcttcc agatgctctg
     6961 gcgcatcgtc atcccgctca cgcgggccgg catcgcctcg accgcaatgc tgtgcgtgat
     7021 cttctcctgg aacgagttcc tcttcagcgt ctcctttacg gcgatagagt ggcagccgct
     7081 ctcggtcgcc gtggccgccc agcagacctc ccgcggccag ttctgggcgc agctctcggc
     7141 gtttaccacc ctggctatac tggttccggt agtcgtcggc tggctcaccc agaagcagct
     7201 ggtgcgcggc ctgaccaccg gcgcggttaa gtagtcccct cgccgggtat cgttcccaaa
     7261 atccccagga ggaggaagaa cgtgacgaag gtaagcgtgc ttggatcggg ggtcatggct
     7321 tccgccatgg ttttcccgct cacggacaac ggccacgagg tcaacctggt aggaacccac
     7381 ctcgacaggg agatcataga ttccatccag aacaccgggg tccacccgga cctggatctc
     7441 gaactccccg aaagcgtaag ggccttccaa cttgaggacg cagaggaggc cttcgagggc
     7501 gccgagatag tcatgagcgg cgtgaactcc tttggcgtga agtgggccag cgagcaactc
     7561 gccggcctac tgaagccggg gatgatggtg atcgccgtta ccaagggcct ggaggctgat
     7621 gacgagggca acctgagcat cctccccgac gttctcgcct cggcggttcc cgaggacctg
     7681 agggcagagg tctcgtggtc cgccatcacc ggccccgcaa tcgccgggga ggtcgccgta
     7741 aagcacgaca cgtgcgtggt cttcgccggg ctcgacgagg gggttctgga gagcctcgcg
     7801 gaaacgtttc gcacggatta ctatcacgtg tggacctcca cggact
//
LOCUS       DBNGVD010000275         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3162569, whole genome shotgun sequence.
ACCESSION   DBNGVD010000275 DBNGVD010000000
VERSION     DBNGVD010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3162569"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1161
                     /locus_tag="AC16CM_07495"
     CDS             352..1161
                     /locus_tag="AC16CM_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3139711.1"
                     /translation="MMHRLLSVVKRHPIVTFFVLTYAISWAFLPIEAVRFLPSGPLIA
                     ALIVIPLTQGWAGLKVLGLRMIRWRVRWYWYVVALGLPLAVHLATAVLNIPFAGVPSL
                     AFSSVTTFLMLFAVRLVNPADGPLGEHPGWNGVALPGLQAERSPLLATMILAPLVAGW
                     HLPLFFLEEGGLQPSILVGGLVTTMAVTFWYAWLFNHTRGSVLMVVVAHSVEGSLQAQ
                     GWIYMGVWLAVAIGLVVFDRKAWRAPAPAEATTQPVRPSRAPREATPAAHA"
     gene            1309..1581
                     /locus_tag="AC16CM_07500"
     CDS             1309..1581
                     /locus_tag="AC16CM_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3139712.1"
                     /translation="MIIMPRNKKWNDLTGRQKAPFVLRGIAQFALLVAALADIYLRPA
                     EEIRGSKWLWGVAAFANFMGIGPIVYFLFGRKHAARRTTDMEGDGT"
     gene            1650..>2270
                     /locus_tag="AC16CM_07505"
     CDS             1650..>2270
                     /locus_tag="AC16CM_07505"
                     /inference="COORDINATES: protein motif:HMM:NF028425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR3139713.1"
                     /translation="MKRLAVLSVVVVLILCSCGAQGQGVGELQRESRSIQPENAQSFA
                     AELRMGAGELNAIGGADTLMEADFAYNVADWEPEVNYEVGSETGELSIEQGGGEDAPF
                     GGDARNEWDLRFNDDVPLDLRVKMGAGESDLDLDSLTLTGLDLEMGAGETKVDLTGDY
                     ARGFAVGIEGGVGEATVLLPSDVGARVEAEGGLGKINAEGLQREGEA"
BASE COUNT          449 a          661 c          768 g          392 t
ORIGIN      
        1 gtacgtagtg cacaggatac gcaaggggtc ttcgaagggg tcttcgccca aacctccgcg
       61 gcaggtaagt cagcacggcg gctaggtccg tcaaggagtt ctgcgagctc tatgccgacg
      121 ccttctatga gtcagcgcca ggacggtagc ggaagtcctg atgaagcgcc agcacccaag
      181 aagtcggtag cagaggcggg cgacacgagc gcctgatgga ggtgagataa agaacattcc
      241 aaaagatcct atccgcaagc cacaggggct tacacagatg tcgcaaaact acgagataag
      301 ggaggttcga cttatgccta cacaacacac tgaaaaaagg aggacaccgg tatgatgcac
      361 cgactcttga gcgtggtgaa acgacacccg atcgttacgt tcttcgtgtt gacttacgcc
      421 atatcgtggg cattcttgcc gatcgaggcc gtgaggttct tgccatccgg accgctaatc
      481 gccgccctca tcgtaatccc cctaacccag ggatgggctg gcctgaaggt gctcggttta
      541 cggatgatcc gctggcgcgt caggtggtac tggtatgtgg tggccttggg tcttccgctc
      601 gcggtccacc tggccaccgc ggtgctgaac atcccgttcg caggcgtccc gtcgctggcc
      661 ttcagctcag tgaccacctt cctcatgctc ttcgcggtgc gcctggtcaa tcccgcagac
      721 gggccgctgg gcgagcatcc gggctggaac ggggtagccc tgccaggttt gcaggccgag
      781 cgctctccgc tactcgccac catgatcctc gcaccgctcg tagcaggctg gcacttgccc
      841 ctgttctttt tggaagaggg tggcttacaa ccctccatct tggtgggcgg tctcgtgact
      901 acgatggccg tgacgttctg gtacgcatgg ctcttcaacc acaccagagg tagcgtgctc
      961 atggtcgtag tggcgcactc cgtcgaaggc agtcttcagg cccagggctg gatctacatg
     1021 ggggtatggt tggccgtggc gattgggctg gtcgtcttcg accggaaggc ctggcgcgca
     1081 cccgcgcctg ccgaggcgac gacacaacct gtgaggccgt ccagggcgcc tagagaagcg
     1141 acaccggcgg cgcacgccta gccccagggc tcgagaagcc cgctccgcag gaggagccga
     1201 gacacgaagc gtccggctcc tcccggaagg gtcgcccgac tgacgcggtc catgggaccg
     1261 gccgcaggct acgcatacag ccgagagaag aggagaaagg ccaaagagat gatcatcatg
     1321 ccgcgcaaca agaagtggaa cgaccttacg ggcaggcaga aagctccctt cgtgctccgg
     1381 gggatcgccc agttcgcact gctagtggcg gcgctcgcgg acatctacct gcgcccggca
     1441 gaggaaatca ggggcagcaa gtggctgtgg ggcgtggcgg cgttcgccaa cttcatgggc
     1501 atcggtccaa tcgtctactt cctcttcggg cgaaagcatg ccgcgcgacg gacaaccgat
     1561 atggaaggag acggaacatg aagaaatcgt ctgcgagggt tgccggatgt agtgagccac
     1621 gcagcagctc cacgagcgaa ggagatggga tgaagcgatt agccgtactg tcggtcgtgg
     1681 tggtcctcat tctgtgttct tgtggggcgc aagggcaggg ggttggcgag ctccagcgcg
     1741 agtcacgctc gatccagccg gagaacgctc aatccttcgc cgccgaactg aggatgggcg
     1801 ctggcgaact gaatgcgatc ggcggggccg acaccctgat ggaggccgac ttcgcctaca
     1861 acgtcgccga ctgggagccc gaggtgaact acgaggtggg cagcgagacg ggcgagctga
     1921 gcatcgagca aggcggtggc gaggacgctc ccttcggcgg cgacgcgcgc aacgagtggg
     1981 atctgcgctt caacgacgat gtgccgctcg acctccgcgt taagatgggg gccggggaga
     2041 gcgacctcga ccttgacagt ctcaccctga cgggattaga cctcgagatg ggggccgggg
     2101 agacaaaggt ggatctcacg ggcgattacg cccgcggttt cgccgtcggc atagagggcg
     2161 gggtcggcga ggccacggtg ctactgccca gcgacgtggg tgcgcgggta gaagccgagg
     2221 gcggcctggg caagatcaac gccgaggggc tccagcggga aggcgaagcc
//
LOCUS       DBNGVD010000276         3700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3890543, whole genome shotgun sequence.
ACCESSION   DBNGVD010000276 DBNGVD010000000
VERSION     DBNGVD010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3890543"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC16CM_07510"
     CDS             <1..620
                     /locus_tag="AC16CM_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3139714.1"
                     /translation="RLELLGWAGRSGSWVIEDDYDAEYRYTGRPLEALQGLDAEGRVV
                     YVGTFSKVLFPALRLGYLVVPPDLTDAFTAARELVDRHPPTAEQAVLAEFIAAGHFGR
                     HLRRMRALYAARQEVLIEEISHHLSGLLDASPAGSGMHLVGWLPAGTDDRKASRQAAR
                     QGVEAPPVSLYRTHESVRGGLMLGYAAVDEERTREGVRRLARALE"
     gene            790..1587
                     /locus_tag="AC16CM_07515"
     CDS             790..1587
                     /locus_tag="AC16CM_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139715.1"
                     /translation="MDHRTSGVAMSYRDLGDGPGDPLVLLHAFPLNGKMFEPQITAFS
                     DDRRVVAPDYPGFGRSPRTPAQPDMRYYAEGLGDLLDRLHLERVVLGGVSMGGYVAFE
                     CMRLFPERISALVLANTRPEPDPAETREARNEMALSVARDGIEVLVELQMERLLAPES
                     LQDEGLVEKVRSIILENSPDGAVAALGAMRERPDSRPFLGEIDVPTLVVGGEEDGISS
                     PEVMGTMAAKIPGARHLTIPNAGHLSNLEAPDEFNAALSDFLKGHGG"
     gene            complement(1655..2113)
                     /locus_tag="AC16CM_07520"
     CDS             complement(1655..2113)
                     /locus_tag="AC16CM_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139716.1"
                     /translation="MKRTRKAGSVVYVPILVVVCALVLAGCKSGSTSDMQQSCRSEGG
                     FLSGREITCTGSVGTAKGEPSVAIIDADGDLSGNYRLEATIKVGKGEAKAYIDTPNGG
                     REGGRVSPGKPLRISAVVGLDDQDDEVSVNLKVLGKQIEDLGYQATVTPL"
     gene            2239..3105
                     /locus_tag="AC16CM_07525"
     CDS             2239..3105
                     /locus_tag="AC16CM_07525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139717.1"
                     /translation="MTTLVHVSDLHFGRPAVSERLDALRGFIGENRPDAVAVSGDLTQ
                     RCSNSEFSKARAYLDSLEEIAPCVVIPGNHDIRWLGSVARNLGFAGLFREKAHRFKYS
                     KYKRHISEELSPSLEVPGAVIAGVNTAHGITRGSITRRFRDLGVIGHVKHTDIVRVSK
                     VFESAAPDAARIVMMHHNPIRGELSGRHGLANTEQALHAFADLGTELILCGHDHQDAI
                     HTIERGAHGLIISTAGTISNRIRPGRASSFNVVEVDDHELHITAHSWRKGAGFVPSDD
                     RTFTRRTASRQT"
     gene            3174..3386
                     /locus_tag="AC16CM_07530"
     CDS             3174..3386
                     /locus_tag="AC16CM_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139718.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDQYLAEEASLRNLQSDKQTI
                     HPRDLPPDQREALSEDIFEILKDTRR"
BASE COUNT          700 a         1252 c         1111 g          637 t
ORIGIN      
        1 gtcgcctgga gttgctgggg tgggcgggac gttccggttc gtgggtgata gaagacgact
       61 acgacgccga gtaccgttac actgggcgcc cgctcgaggc tcttcagggg ttggacgcgg
      121 agggccgggt ggtctacgtg ggaaccttca gcaaggtcct ctttccagcc ctccggctcg
      181 gctatctggt cgtgccacca gatctcacgg acgccttcac ggcagcccgc gagctcgtcg
      241 acaggcaccc gcccaccgcg gagcaggccg tgctcgccga gttcatcgcc gccggacact
      301 tcggtaggca cctgcgccgc atgcgcgccc tctacgccgc caggcaagaa gtcctgatag
      361 aagagatctc tcaccacctc tccgggcttc tcgacgccag ccctgccgga tcgggcatgc
      421 acctcgtggg gtggcttcca gccggcaccg acgaccggaa ggcctctcgg caggccgcgc
      481 ggcaaggcgt cgaagcgccg ccggtctcgc tctacagaac ccacgaaagc gtcaggggtg
      541 gtctcatgct cggctacgcc gcggttgacg aagagaggac acgcgaagga gtacgccgcc
      601 tggcaagagc gctggagtag aggcatcagg tcacaggttg caggcttcag taaagggatg
      661 aagccggaaa cgctccgccg aaaacgttac catcaggggc ggcgatggga taacagagag
      721 gtcaccttag ggccaaaacc tgaaacctga agcctgtaac ctgtagccta ttcaaaagga
      781 gagacccggg tggatcacag aacttccggc gtggcgatgt cgtacaggga cctcggagat
      841 gggccgggcg acccgctggt cttgctccac gcctttccgt tgaacgggaa gatgttcgag
      901 ccccagatca cggcgttttc ggacgatcgg cgcgtcgtgg ccccggatta tcccgggttc
      961 gggcgttccc cgcgcacacc cgcccagcca gacatgcgct actacgcaga agggctcggc
     1021 gacctgctcg acaggctgca cctggagcgt gtggttctcg gcggcgtctc catgggcggc
     1081 tacgtcgcct tcgagtgcat gaggctcttt cccgagagaa tctccgccct cgtgctcgcc
     1141 aacacccggc ccgagcccga cccggcggag acgcgcgagg cccgaaacga gatggccctg
     1201 agcgtggcca gggatggcat cgaggtgctc gtcgagctcc agatggaacg cctcctggcc
     1261 cccgagagcc tgcaagacga gggcctcgtg gagaaggtgc gctccatcat cctcgaaaac
     1321 tcccccgacg gcgccgtggc cgccctcggc gccatgcgcg agcgtcccga ctcgcggccg
     1381 ttcctcggcg agatagacgt tcctaccctc gtggtcggcg gtgaggagga cggtatctcc
     1441 tctcccgagg tcatgggcac tatggccgcc aagatacccg gcgccaggca cctgaccatc
     1501 cccaacgccg gccacctctc caacctcgaa gccccggacg agttcaacgc ggccctgagt
     1561 gatttcctca agggacacgg cggataaggc ttcaggcaac aggcttcagg aaaattacct
     1621 gttgcctgaa gccggggggg ttcgcgaacc gcccctacag cggggtcacg gtagcctggt
     1681 agcccagatc ctcgatctgt ttgccaagca ccttgaggtt cacggagacc tcatcatcct
     1741 ggtcgtcgag gcccacgacg gcgctgatgc gcaacggttt accgggggag acccttcccc
     1801 cctctctccc gccattgggg gtgtcgatgt aggcttttgc ctcacccttg cccactttga
     1861 tggtggcctc cagcctgtag tttccggaca ggtctccgtc ggcatcgatt atggcgaccg
     1921 agggttcgcc tttcgctgtg cccacgctgc cggtacacgt tatctctctc ccggagagga
     1981 agccgccctc ggatctgcag gactgttgca tatccgaggt gctgccagac ttgcaccccg
     2041 ccagcacgag cgcgcagacc acgacgagga tgggcacgta caccacgcta ccggccttcc
     2101 tggttctctt catgctttcc tccttctcgc cctctgtgat agtagcgaga aagcctattc
     2161 cacgctcgcc tgtgagcgcc ggctgcccga tggggccaac gtttgtcgag ctggcgtgtt
     2221 cgggtagaat cgatattcgt gaccaccctc gtgcacgttt cggacctgca tttcgggcgg
     2281 cccgcggtct cagagcgtct cgacgccctg agagggttca tcggggagaa ccggcccgac
     2341 gcggtagccg tctccggtga cctgacccaa cgctgttcga actcagaatt ctcaaaagcg
     2401 cgtgcctacc tcgattcgct cgaagagata gcaccatgcg tggtcattcc cggcaaccac
     2461 gacatccgct ggctcgggtc tgtggcccgc aacctcggtt tcgcggggct attcagggag
     2521 aaggcccacc gcttcaaata ctccaaatac aagaggcaca tctcggagga gttgagcccc
     2581 tcgctcgagg tgccaggcgc ggtcatcgcc ggcgtcaaca cggcccacgg catcacgcgg
     2641 ggctcgatca cgcgtcgctt cagggacctc ggtgttatag ggcacgtcaa gcacaccgac
     2701 atagtgcgcg tcagtaaggt cttcgagagc gccgccccag acgcggcccg catcgtgatg
     2761 atgcaccaca atcccataag gggcgagctc tccggccgcc acggcctcgc caacacggag
     2821 caggccctcc acgccttcgc cgacctcggc acggagctta tactctgcgg ccacgaccac
     2881 caggacgcga tccacaccat agagaggggc gcccacggcc tcataatctc gaccgcggga
     2941 acgatctcca accgcatccg accgggccgc gcctccagct tcaacgtggt cgaggtagat
     3001 gaccacgaac ttcacataac ggcccactcc tggcgtaagg gggctggctt cgttccctcc
     3061 gatgaccgca ccttcacccg ccgcacggcc tccagacaaa cctgaaaccc acaacccatc
     3121 ccgacgatat aatctgtcgg ataccaacca cccgcacgga agggacgacc tgcatgtgga
     3181 gcagattgac ccgcggcggc gacgggctgc cccgcgagaa ggttgacgcc atagtccgtc
     3241 tcatagacca gtacctggcc gaagaggcaa gcctgcgcaa cctccagtcc gataaacaaa
     3301 ccatccaccc ccgcgacctc ccccccgacc aacgagaggc cctgagcgaa gacatcttcg
     3361 agatcctcaa agatacacgc cgatagagca cgctccggct tcgcctacgc tttcagcacg
     3421 ctccggcttc gcctacgctt gtcagcacgc tccagcctgg cggctgtcgc ttgtcggcaa
     3481 cgcttcggct acgcctcgct ttcggccagt cgtcccgatt cgcatcggta gcttcgacat
     3541 aaggctttca cgggtatcga agcttcaaag cggcagggtg caggccgcaa cgggatctgg
     3601 ctgacagcga gccctgtaag ggcgaagcgt tgccgacaag cgacaaccgc aaggctggag
     3661 cgtgctgaca agccggcgta gcgaagccgg agcgtgctct
//
LOCUS       DBNGVD010000277         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2980535, whole genome shotgun sequence.
ACCESSION   DBNGVD010000277 DBNGVD010000000
VERSION     DBNGVD010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2980535"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="AC16CM_07535"
     CDS             <1..654
                     /locus_tag="AC16CM_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3139719.1"
                     /translation="VLDPEDPNKVIVDSPEAIAGLATERSMITDGVSPQAVTVYKEDE
                     SAGAFLNGDAVFLRNWPYVYALIGTSDYPKLDTDQVGVSELPSGDGKPGNGTVGDQPL
                     YISSSSKNPDAAWKFIEFLSSPEQQKLRAIEGSYLPTRSDLYDDPEIQESVPVVSLAR
                     TALQHTLPRPVTPYYSDMSLEMQEQFNSSLLGDVSPAEAARTLKANLEDFIRQGQDS"
     gene            683..1657
                     /locus_tag="AC16CM_07540"
     CDS             683..1657
                     /locus_tag="AC16CM_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3139720.1"
                     /translation="MRIGLVGAGFMGGVHLNAYANIPEVEVVGVADANRESAAGAAQL
                     AGARPYSSYEELVAAEDVDVVDVCLPTAFHKDLAVKAARDGKHVILEKPIARTIEDAA
                     EILESFSGGGPRLFVGHVVRFFPEYVRIKEKIDAGDLGTIGVVRTTRRSPLLRGWNDW
                     YADWRVSGGVLVDLVIHDFDFLRWALGDVERVYARGVLGREYNRLDYALVTLRFAGGA
                     IAHVEAHWGYPGPFNYSIEVAGSRAMLTAGSTEPATLELVGSAEQAPGLASGPGPYER
                     ELAHFVRCIATGEEPVVKPEDAYEALRISLAATESIFGGKPVTLGERP"
     gene            1654..>2317
                     /locus_tag="AC16CM_07545"
     CDS             1654..>2317
                     /locus_tag="AC16CM_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3139721.1"
                     /translation="MSVRVGMMSFAHVHAPAYAAVLANLEMADFVGLTDTDADRGREA
                     AERYGVRYFEDAESLLGDVDAVVICSENRNHARDAIPALQAGVHVLCEKPISTTLDDA
                     RAIISASGASGSQLRTAFPVRYLPSIVRAREIVRDGSLGRILAINGTNHGRIPGGWFV
                     DPELAGGGAVMDHTVHLADILRWMLDVEVRSVYAEVDSFFGATETDDAAILTLELEGG
                     PFA"
BASE COUNT          419 a          757 c          755 g          386 t
ORIGIN      
        1 gtactggatc ccgaggaccc gaacaaggtc atcgtggaca gtcccgaggc catagcaggc
       61 ctcgccacgg agcgcagcat gatcaccgac ggcgtctcgc cgcaggcggt tacggtctac
      121 aaggaggacg agtcggccgg ggccttcctg aacggcgacg cagtcttcct tcgcaactgg
      181 ccctacgtgt acgccctgat cggcacctcc gactatccga agctggacac cgaccaggtc
      241 ggcgtttccg agctaccgtc aggcgacggt aagcctggca acggaacagt gggcgaccag
      301 ccactgtaca tcagctccag ttccaagaac ccggatgccg cgtggaagtt catcgaattc
      361 ctctcctcgc ccgagcagca gaaattgagg gcgatcgagg gctcgtacct gccgaccagg
      421 tccgacctct acgacgaccc ggagatccag gagagcgtcc ccgttgtctc cctcgccagg
      481 acggccctgc agcacaccct tccacgtccc gtaacgccgt actactcgga catgtccctg
      541 gagatgcagg aacagttcaa ctcctcactg cttggcgacg tcagccctgc ggaagcggca
      601 cgcaccctga aagcgaacct cgaggacttt atccgtcagg gccaggattc ctagccatga
      661 gcgataccag aggaggaacc gagtgcggat cggcctcgta ggagccggct ttatgggcgg
      721 ggtgcacctg aacgcctacg cgaacatccc agaggtagag gtggttggcg tcgcggacgc
      781 caacagagaa agcgccgcgg gtgcagccca gctagccggg gcgcggccct actcttccta
      841 cgaggagctc gtcgcggctg aggacgtcga tgtggttgac gtgtgcctgc ccaccgcctt
      901 ccacaaggac ctcgccgtga aggccgcacg cgacggcaag cacgtcatcc ttgagaagcc
      961 gatagcccgc accatcgaag acgcggcgga gatcctggag tctttctccg gcggcgggcc
     1021 gcggctcttc gtcggccacg ttgtgcgttt cttccccgag tacgtgcgca taaaggagaa
     1081 gatcgacgcc ggagacctgg ggaccatcgg ggtggtgcga actacccgtc gctccccgct
     1141 tcttcggggt tggaacgact ggtacgcgga ctggcgcgtg agcggcggcg tgctggtcga
     1201 cctcgtgatc cacgacttcg atttcctgcg gtgggcgctt ggcgacgtgg agagggtgta
     1261 cgcccgcggc gttctgggac gcgagtacaa ccgcctggac tacgccctgg tcaccctgcg
     1321 ctttgcgggc ggcgccatcg cacacgtcga ggcgcactgg ggataccctg gcccgttcaa
     1381 ctactcgatc gaggtggctg ggagccgtgc catgctgacc gccggcagca ccgagccagc
     1441 aacgcttgaa ctggtcggct ccgcggagca agcgcccggc ctcgcttccg gtcctggacc
     1501 ttacgagagg gagctcgcgc acttcgtccg ctgcatcgcc accggggagg agcctgtcgt
     1561 caaaccggaa gacgcctacg aggcactcag gatcagcctc gccgcaacgg aatctatctt
     1621 tggggggaaa ccggtgacgc tgggggagcg gccatgagcg tcagggtcgg catgatgagc
     1681 ttcgcccacg tccacgcccc agcctacgcc gccgtgttgg cgaacctcga aatggcggac
     1741 ttcgtcggcc tgaccgatac ggacgcggac cgtggccggg aggcagccga gcgttacggc
     1801 gtgcgttact tcgaggatgc cgagtcgctg ctcggggatg tggacgcggt ggtgatctgc
     1861 tccgagaaca ggaaccacgc cagggatgcg atccccgccc tgcaagccgg cgtgcacgtg
     1921 ctctgcgaga agcccatctc tacaacgctc gatgacgcaa gggccatcat atccgcctcc
     1981 ggggcctccg gcagccagct caggactgcc tttccggtcc gctaccttcc ctctatcgtg
     2041 cgcgcgaggg agatcgtgcg ggatggttca ctgggccgta tcctggcaat aaacgggacc
     2101 aaccacggcc ggatcccggg cggctggttc gtagatcccg agctggccgg cggtggcgcg
     2161 gtgatggacc ataccgttca cctcgccgac atactgcgct ggatgctcga cgtggaggtc
     2221 agaagcgtct acgcggaggt cgactcgttc ttcggggcca cggagacgga cgacgccgcc
     2281 atcctcaccc tcgagctaga aggcgggcca ttcgccg
//
LOCUS       DBNGVD010000278         2473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2251193, whole genome shotgun sequence.
ACCESSION   DBNGVD010000278 DBNGVD010000000
VERSION     DBNGVD010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2251193"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="AC16CM_07550"
     CDS             <1..1017
                     /locus_tag="AC16CM_07550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3139722.1"
                     /translation="LLTSDPTDRRTLPALVRLLKVYGPKLTTRSYLDGLSWLPAGLKE
                     IIAIHTLNAGVAPGRTPALYASMPAIMATDGVFVPEGGVYEIVLALGMLARHAGVEIE
                     TDVPVREIRDGTVVTAERKYDADAVVSDLDEGRLEGLLVRGKRSEQQGLSCSGIAVYA
                     ALRKELPTGIGAHGVVLPSDPGDLYASLEAGEEPRETMAFVNYYRGGEIYPNDGGTLA
                     ILLTAPANGREYGLKDPFVAREIERVSEAVGLPGPATGYFGDYGLLDPAYFGGWGSKG
                     GALYGSVRPFWRSGPLHRPRYNERGRPWLWRVGASVHPGGGIPAVLGGAMISTDRMLR
                     SLPR"
     gene            1047..2186
                     /locus_tag="AC16CM_07555"
     CDS             1047..2186
                     /locus_tag="AC16CM_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3139723.1"
                     /translation="MNLGNQRHLFEIPDDVAYLNCAYMSPQLRRAREIGGWSVTRKSR
                     PWEITPDDFFREVEEVRTLFARLVGGDAEGVAVVPSVSYGISVAAANVPVGDGEKIII
                     LQDQFPSNVYSWRELASQKGAKLVTVPRPEDADWTRALLAQIDEHTAVVAAPNCHWTD
                     GSLVDLIQVGERVREVGAALVIDGIQSVGAMPFDVSEVRPDFLIAASYKWLLGPYGVG
                     FMYVDEGYREGQPIEHNWVNRHKSEAFSQLVDYQDAFQPGARRYDVGERSNFVLLPMA
                     AEALRQLLDWGVGNTYETIGALTDLIERAAEERGIATIPRDRRARHLIGLMLGSDAPD
                     DLATRLMHHGVYVSVRGPSVRVSPHLYNTERDIERFFDALGEITA"
     gene            2263..>2473
                     /locus_tag="AC16CM_07560"
     CDS             2263..>2473
                     /locus_tag="AC16CM_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3139724.1"
                     /translation="MLIGLQVPRFTWPQGTSGIGPKLAEIGRTADEAGFASLWVMDHF
                     FQIEAVGEIDEPMLEGYSALTYLAGV"
BASE COUNT          435 a          785 c          841 g          412 t
ORIGIN      
        1 ttgcttacgt ccgaccccac agatcgcagg acactacccg ccctggtccg gctcctcaag
       61 gtgtacgggc cgaagttgac gacccgctcc tacctcgatg gtctctcgtg gttgccggcg
      121 gggttgaagg agataatcgc catccacacg ctcaacgccg gggtcgcgcc gggaaggacg
      181 cccgccctgt acgcgagcat gcccgcgatc atggccactg atggcgtttt cgtgccggag
      241 ggtggcgtgt acgagatcgt gctcgccctt gggatgctcg ccaggcacgc cggggtcgag
      301 atagagacgg atgtaccggt gcgtgagata cgagatggca cggtggtgac ggccgaacgt
      361 aaatacgacg ccgatgcggt ggtgagcgat ctcgacgagg gccggctgga aggtttgctc
      421 gtccggggta aaaggtccga gcaacaaggt ctctcctgct ccgggatcgc cgtttacgcg
      481 gcgctgcgga aggagttgcc caccggtatc ggggcgcacg gcgttgtgct gccttccgac
      541 ccgggcgacc tgtacgccag cctcgaagcc ggggaggagc cgcgggagac gatggccttc
      601 gtcaactact atcgcggggg cgagatctac ccgaacgacg ggggaacgct agccatcctg
      661 ctcacggccc cggcgaacgg ccgggagtac ggcctgaaag atcccttcgt ggcgcgggag
      721 atcgaacgcg tctccgaggc cgtcgggcta cccggtcccg ccaccggcta cttcggggat
      781 tacgggctcc tcgatcctgc gtacttcggt ggctggggaa gcaagggtgg ggccctctac
      841 ggttccgtgc gtcccttctg gaggagtggg cccctgcaca ggccccgtta caacgagcgc
      901 gggcgtccct ggctgtggcg ggtcggagcc tcggtgcatc ccggtggagg catacccgcc
      961 gttctcgggg gggccatgat ttccaccgac aggatgctcc ggtccctgcc gaggtgaaga
     1021 cgcccgagag aaggaggagg acagggatga accttggaaa ccagcgccac ctgttcgaga
     1081 tacccgacga cgtcgcctac ctgaactgcg cctacatgtc gccgcagcta cgccgggcgc
     1141 gcgagatcgg ggggtggtcc gtaacgcgca agtcacgccc ctgggagata acgccggacg
     1201 acttcttccg ggaggtggaa gaggtccgca ccctgttcgc gcggctcgtc ggcggggacg
     1261 ccgagggcgt cgccgtcgtt ccctccgtca gctacggaat ctccgtcgcg gcggccaacg
     1321 tgccggtagg cgacggggag aagatcatca tcctccagga ccagtttccg tccaacgtct
     1381 actcctggcg cgagctggcg tcacagaagg gcgcgaagct ggtcaccgta ccccgccccg
     1441 aagacgccga ctggacgcgc gctctgctcg cgcagatcga cgagcacacc gcggtggtcg
     1501 cggcaccgaa ttgtcactgg accgacggtt cgctcgtgga tctcatccag gtcggcgagc
     1561 gggtcaggga ggtcggggcg gcgctcgtga tcgacggcat acagtctgta ggcgccatgc
     1621 ccttcgacgt ctcggaggtg aggccggact tcctgatcgc cgcctcgtac aagtggctgc
     1681 tagggcccta cggcgtcggt ttcatgtacg tcgacgaggg ataccgcgaa ggccagccca
     1741 tcgagcacaa ctgggtgaac cgccacaaga gcgaggcctt ctcacaactg gttgactacc
     1801 aggacgcctt ccagcccggc gcccgtcgct atgacgtggg ggagcggagc aacttcgtcc
     1861 tgctcccgat ggccgccgag gccctccggc agctcctcga ctggggggtc ggaaacacct
     1921 acgagaccat cggcgcgttg acggatctta tagaacgagc ggcagaagaa cgcggtatcg
     1981 cgacgatccc cagagacagg cgggcgcggc acctgatcgg cttgatgctt ggctccgatg
     2041 ccccggacga cctcgccacc aggctcatgc accacggcgt ctacgtcagc gtgcgcggcc
     2101 cgagcgttcg cgtctcaccc cacctctaca acaccgaaag ggacatcgag cggttcttcg
     2161 acgcgctggg ggagatcacc gcctagggtg ccctaccatg gctcgttccg tgctcggcgt
     2221 atagactccg ccggtcggat ctaccagaga ggagagggag acatgcttat aggtttgcag
     2281 gtaccgaggt ttacctggcc ccagggcacc tccggcatag gcccgaagct ggccgagatc
     2341 ggcaggaccg ccgacgaagc agggttcgcg agcctgtggg tcatggatca ctttttccag
     2401 atagaggctg tcggcgagat cgacgagccg atgctggagg gctacagcgc actcacctac
     2461 ctggcaggcg tca
//
LOCUS       DBNGVD010000279         4420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2069215, whole genome shotgun sequence.
ACCESSION   DBNGVD010000279 DBNGVD010000000
VERSION     DBNGVD010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2069215"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="AC16CM_07565"
     CDS             <1..362
                     /locus_tag="AC16CM_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3139725.1"
                     /translation="LDRENEYPDRLSGGQQQRVAIVRALAMRPHLMLLDEVTSALDPE
                     LVAEVLSVIKGLAQEGMTMLIATHEMGFARDIADRICFLEAGRILEQGPPSKIFTSPE
                     NPRTRQFLSRIIEAGRL"
     gene            831..1031
                     /locus_tag="AC16CM_07570"
     CDS             831..1031
                     /locus_tag="AC16CM_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139726.1"
                     /translation="MMGRQTVRISVEVRSGGARFRVGVQARSIREALRAVGGRYPRGV
                     VAVAFPEPGGFLAHVQTGQEAA"
     gene            1213..1740
                     /locus_tag="AC16CM_07575"
     CDS             1213..1740
                     /locus_tag="AC16CM_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139727.1"
                     /translation="MVGYVNIEEQVDFDFTRARHRAFLRRMRARVFGDPWSTRMQSLE
                     DASGGIGAFNRVRLGRKVVAVEKIVGSVGRFRDFDRDFLPTRQSLETKWKRVDRAYHR
                     GQNLPPVSLFEVDGRYFVEDGNHRVSVARYQGVEWIDAEVRVLHGDMPATPVREAGLE
                     TMAGEQMVDPQPIAA"
     gene            1788..3515
                     /locus_tag="AC16CM_07580"
     CDS             1788..3515
                     /locus_tag="AC16CM_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3139728.1"
                     /translation="MITSIQSRANVGRVLTVTVLVVALAAFLMLAATPSYASTTFYVN
                     STSDRPDANLGAPDWDTGYTVERDGGGEEAECTLRAAIEQANSTQGADTIKFAIPGTG
                     VKTIAPQSELPLIAESLTIDGYTQPGSSPNTRAVGSDAVLRIELSGPANGHGLVIEAP
                     NSTVRGLVVNSSGEGIRITGSGATGNRVEGNYLGTDPSGTLDLGHGDYGIAIGGASNN
                     TIGGTTPGARNLISGNLGGIGIEGDGATGNKIVGNYIGTDRTGTVDLGNRSSGVYIDD
                     APNNTVGGTVAAARNIISGNDAYGVQIEGSGATGNEISGNYVGTDRSGAGPLENADGV
                     YIDNAPGNTVGGTTVGARNVISGNDSEGVVILGPGATGNRVAGNYVGTGATGQRVPGH
                     NDSGITILNAANNVVGGREAGARNTISGNGVGVFIQGDGATGNRVLSNSIFGNAALGI
                     DLYGVFGPNPNDPDDKDTGPNGLQNKPVLSSARKSSTGTTTVRGTLDSTPGKTFLVQF
                     FSNPEGGDEGKTLLGSTKVSTSSSGDASLSFSTRKLVRLGQNITATATNTSTNSTSEF
                     SAPRKVVAR"
     gene            3576..>4420
                     /locus_tag="AC16CM_07585"
     CDS             3576..>4420
                     /locus_tag="AC16CM_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3139729.1"
                     /translation="MTIQITTQRAETRVRTSRSLLLGLGLGVALAGAVALGGTAHEAE
                     AAFTEKIVFSSDRTAGADNPTGDYEVFKMNPDGTGVRQLTFNEADDWDPTLSPDGRRI
                     SYETNTTSSNPEGDWEVYTMSALDGGDKRNLTNNDAGVDDYNPVFSPGGKRIAYATEG
                     IQQTNPEGDPEIYVLNASNGTDNKNLTNNGLDLDGVYLIEDYNPTFSPDGTKIAYTSL
                     GKQPSNPEGDDEVYRLRALDGTGKKNLTNNGPDVDDYLPRFSPDGKKIAYESDGVQNS
                     NPQGE"
BASE COUNT          959 a         1384 c         1405 g          672 t
ORIGIN      
        1 tgctggacag ggagaacgag tacccggacc ggctctccgg cggccagcag cagagggtgg
       61 ccatcgtgcg cgcgctcgcc atgcgccccc acctcatgct gctcgacgag gtaaccagcg
      121 ccctcgatcc ggaacttgtc gctgaggtcc tctccgtcat caaggggctg gcccaagaag
      181 gcatgacgat gctcatcgcc acccacgaga tgggtttcgc gcgtgacata gccgaccgga
      241 tctgctttct ggaggccggc cgtatcctcg agcagggccc accgagcaag atcttcacct
      301 ccccggagaa cccgcggacc cgccagtttc tcagccgcat catcgaggca ggacgcctgt
      361 gagcacgctc cggctaacgc ctccgcgtgc tcgaaatgat cagaaatatg tagtcgaggg
      421 tctggcgcga gattcgtgag ggatgatact atgaggttgg ttgttgtacc tcgattagga
      481 ggaaagggtc cagtggacta gtttgtcaac agccatctgc gaactgttac gagttcgcgg
      541 accgggtcgg tggtcgtctg ctaaaagggc ggctcgccgg cccggatatt ttttgttttc
      601 ggctttgagc tatcagccat cagctatcaa cttccagcca gttctcgttt gccgctcgtg
      661 gcgaggactt gggttcttat agcccccgtt tttgatcttt ctggttctcc atggcccgtt
      721 cgggccatct gccttcgtgt ccgaaacatg gcccagacag gtgaagtcgc tcgtctccac
      781 ggtcgcttga atagagggtg caaggttcac cgagagacga gaggagaacg atgatgggac
      841 ggcagacggt gaggatctcc gtggaggtca ggagcggcgg cgcacgcttc cgggtgggcg
      901 ttcaggccag gagcatcagg gaggcgctgc gcgcggtggg aggccggtat ccacgggggg
      961 tagtcgcggt ggctttcccg gagcccggag gctttctcgc tcacgtgcag acggggcagg
     1021 aggccgcctg agaagcctgg aaatggaaga cgagcccacg gttaccccac aagagaaccc
     1081 aggataacga aatgtccgaa tcattcgcgc aacagctcgt acaccagcag cgcgagaacc
     1141 ccgcggagat cgccttcacc gcaccagacg gcccgaacgg cgacgctcca caacccacgt
     1201 gaggaacgaa gcatggtcgg atacgtgaac attgaagagc aggtcgactt cgacttcacc
     1261 cgcgcccgcc acagggcgtt tctccgccgc atgcgagccc gggtcttcgg agatccctgg
     1321 tccacccgta tgcagtcgtt ggaggacgcg agcggcggga tcggcgcgtt caacagggtc
     1381 cgccttggca gaaaggtcgt ggccgtcgag aagatagtgg gcagcgtggg gcgtttcagg
     1441 gacttcgatc gggacttcct gccgacccgc cagagcctgg aaacgaagtg gaagcgcgtg
     1501 gaccgcgcct accacagggg acagaacctg ccccccgtct cgctcttcga ggtggacggt
     1561 cggtatttcg tggaggacgg caaccaccgc gtgagcgtgg cgcgctacca gggcgtcgag
     1621 tggatagacg ccgaggtgag ggtcctgcac ggcgatatgc ctgcgactcc cgtgcgggag
     1681 gcaggcctgg aaacgatggc gggcgaacag atggtggacc cgcaacccat tgcggcatga
     1741 gcgtacttcc gggctcgaag gctacagaga tgaaaggaac aagacagatg atcacttcga
     1801 tacagagcag ggcgaacgta ggaagagtac tgacggtaac cgtcctcgtg gtggcgctcg
     1861 cggcgttcct catgctggct gcaactccct cctacgcctc gaccaccttc tacgtcaact
     1921 caacctcaga ccgccccgac gccaacctgg gggctcccga ctgggacacg ggttacaccg
     1981 tggagaggga cgggggcggc gaggaggccg aatgcacgct cagggctgcc atagagcagg
     2041 ccaactcgac ccagggggca gacaccatca agttcgccat ccccggcacc ggtgtgaaga
     2101 ccatcgcccc ccagagcgag ttgcccctga tcgccgagtc gttgacgata gacggctaca
     2161 cccagcccgg ttcatcccca aacaccaggg ctgttggcag cgatgcggtc ttgaggatag
     2221 agctcagcgg gcccgccaac ggtcatggcc tcgtgatcga ggcgccgaac agcaccgtca
     2281 gggggctggt cgtcaacagt tccggtgagg ggatacgcat caccggttcc ggcgccaccg
     2341 gcaacagggt ggagggcaac tacctgggca ccgacccttc tggcaccctg gatctcggcc
     2401 acggcgacta cggaatcgcc atcggcgggg cgtccaacaa caccatcggg ggaaccacac
     2461 cgggagcgcg caacctaatc tcaggcaacc ttggtgggat cggcatcgag ggcgatggtg
     2521 ccacgggcaa caagatagtg ggcaactaca taggcaccga cagaaccgga accgtcgatc
     2581 tgggcaacag gtcgagcggc gtatatatcg acgatgcgcc aaacaacacc gtcggaggca
     2641 ccgtggccgc ggcgcgcaac atcatctctg gcaacgacgc ttacggcgtg cagatcgagg
     2701 gtagcggtgc cacaggcaac gagatctcgg gcaactacgt gggcacggac aggtcaggcg
     2761 caggccccct ggagaacgcg gacggcgtat atatcgacaa cgcgccaggc aataccgtcg
     2821 gggggaccac ggtcggggct cgcaacgtca tctccggcaa cgacagcgag ggcgtagtga
     2881 tcctgggccc gggcgccacc ggcaacaggg tggctggcaa ctacgtgggt accggcgcca
     2941 ccggacagag ggttccgggc cacaacgact cgggcatcac catcctgaac gcggccaaca
     3001 acgtggtcgg aggccgggaa gctggggcgc gcaacaccat ctccggcaac ggagtcggcg
     3061 tcttcatcca gggggacggt gccaccggca accgtgtcct ctctaactcc atcttcggca
     3121 acgccgccct cggaatagac ctctacggcg tgttcgggcc gaaccccaac gatcccgacg
     3181 acaaggatac cggccccaac ggcctgcaga acaaaccggt cctctcctcc gccaggaaga
     3241 gctccaccgg taccacgaca gtcaggggca cgctcgacag caccccgggc aagaccttcc
     3301 tggtgcagtt cttctccaac cccgagggag gggacgaggg caagaccctc ctcggctcga
     3361 cgaaggtttc caccagcagt tcgggcgacg cctctctctc cttctcgacc aggaagctgg
     3421 tcaggctggg gcagaacata acggccaccg ccacaaacac ctcaacgaac agcacctcgg
     3481 agttctcggc acccaggaag gtggtggcga gataggagaa aggcgtaaag aagagcggct
     3541 aaaccagtta cgtacagaga gaaaggaaac ctgagatgac catccagatc acgacacaga
     3601 gagcggaaac acgggtcagg acgagtagat ccctgctcct cggcctcggg ctcggggtgg
     3661 cgctggccgg ggcggtggcg cttgggggta cggcccacga ggcggaggcg gccttcaccg
     3721 agaagatagt cttcagctcc gaccgaaccg ctggcgcgga caaccccacc ggcgactacg
     3781 aggtcttcaa gatgaaccct gacggcacgg gcgtcaggca gctcaccttc aacgaggccg
     3841 acgactggga tcccacgctc tcccctgacg gcaggaggat ctcctacgag accaacacta
     3901 cctcctccaa cccggagggc gactgggagg tctataccat gagcgccctc gacggcgggg
     3961 acaagcggaa cctcaccaac aacgacgccg gcgtggacga ctacaacccg gtcttctctc
     4021 cgggaggcaa gaggatcgcc tatgcgaccg agggcatcca gcagacgaac ccggaaggcg
     4081 accccgaaat ctacgtcttg aacgcctcca atgggacgga caacaagaac ctgacgaaca
     4141 acggcctgga tctggacggc gtctacctga tcgaagacta caatcccacc ttctcaccag
     4201 acggcacgaa gatcgcctac acgagcctgg gcaagcagcc ctccaacccg gagggcgacg
     4261 atgaggtcta ccgcctgaga gccctggacg gcacgggcaa gaagaacctc acgaataacg
     4321 gcccggacgt cgacgactac ctgccaaggt tctcccccga cggcaagaag atcgcttacg
     4381 agagcgacgg cgtgcagaac tccaaccccc agggtgaaga
//
LOCUS       DBNGVD010000280         4435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3344296, whole genome shotgun sequence.
ACCESSION   DBNGVD010000280 DBNGVD010000000
VERSION     DBNGVD010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3344296"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="AC16CM_07590"
     CDS             <1..1031
                     /locus_tag="AC16CM_07590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3139730.1"
                     /translation="SSSMARRLMRRIGLALLGLIVVLLTLAVLGAIYQSIATQLAERA
                     YPPPGEMVDVGGYSLHINCVGQGSPTVILDAGSGLFSAQWVRVQRQVSSATRVCAYDR
                     AGMGWSEMGPGPRDAKQITSELHTLLSKAGIEGPYVLVGHSFGGMYMQTYAARYPDEV
                     AGVVLVDSSTDPDQFSQRPEAQDSNEPQKQTSAVVSQLVRFGISLPARVGIVRLLSYL
                     DRASPELPQEQRAQTDALTPSTREFTTSVLEFLAPTQTLRLGSPGSLGNKPLAVVTAP
                     KQAEPGWIKHQDKLATLSTNSIHRVVKGATHESLLYDRSDAQSTSAAIVEVVGVVRND
                     RPLAQENH"
     gene            complement(1166..1813)
                     /locus_tag="AC16CM_07595"
     CDS             complement(1166..1813)
                     /locus_tag="AC16CM_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139731.1"
                     /translation="MRARALMRRMMWLAKGAAIFWGAVLTIALMLGAGTVAIAAVPGD
                     PFNLGQTNTINNALTKLAGSRDGGAMLTVDNDSSASGSRALDLRVEDGRPPINVNAVA
                     GKATNLNADKLDGKDSEEIGVNGLELVEEDSSTNSDSSKTLRADCPDEKVLVSGGYEL
                     IGGTSGSFLQLQTDVVVTDVELSTATQSVDVTAFEENPTSADWLVRASALCATAP"
     gene            complement(2198..2581)
                     /locus_tag="AC16CM_07600"
     CDS             complement(2198..2581)
                     /locus_tag="AC16CM_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139732.1"
                     /translation="MAAIAKAMGFPPGLWFEELGLGIQADSAEKGQNLADKVDLLLNT
                     ITNDKTGSPYTDAEVARMSLGVLTEDEVAGIRAGMIPSPTVDKLVALARVLGVEAGYF
                     LDGLEEPPLLDREALDYFVTRRRAP"
     gene            2991..3587
                     /locus_tag="AC16CM_07605"
     CDS             2991..3587
                     /locus_tag="AC16CM_07605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139733.1"
                     /translation="MTGDPVYTTRDLGLDTWDDFARLVEANNGVWGGCWCMGFHPEGL
                     DREDASANRDAKRAHVERGTVHQVLVYADEDCVGWCQYGTAAELPNIQNRKAYAKDLV
                     VLPAWRIGCIFTDRSHRGQGVARAAVGAALAAIEASGGGIVEAYPEQIEGRPPQRGSY
                     LHTGPETLFEEFGFRRDRRISKWRWVMRLRVDAPSRPG"
     gene            complement(3681..>4435)
                     /locus_tag="AC16CM_07610"
     CDS             complement(3681..>4435)
                     /locus_tag="AC16CM_07610"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR3139734.1"
                     /translation="GKPAAMSVPGGCGIPFLWGSAIYPHDSRRASAKGGSGQAGRMSG
                     SGNRLPDVILYGGVIACLALLALVSRGDVGLIRGAKILFGGTLLVVALAAVALASRHL
                     WNTPTTPTALELSRCFESVRSMGGARFEAFMADLFRAMGHRVQPRGGPGDQGVDIIID
                     LGKGRIAVQCKNHAVPVGNRPVQEVYAGARHHRCVEAWVVAPAGYTRGARALANSTGV
                     SLHDADAIRRWIKTVDAIERQRADGTPTRTGK"
BASE COUNT          777 a         1405 c         1399 g          854 t
ORIGIN      
        1 tgagcagcag tatggctaga aggcttatgc ggcgaatcgg gttggcgctc ctggggttga
       61 tcgtcgtcct gttgaccctc gctgtcctcg gggcgatcta ccaatccatc gctacacagc
      121 ttgccgagcg agcctatccc ccgcccggtg agatggtcga cgtgggaggc tacagcttgc
      181 acataaactg cgtgggccaa ggcagcccta ccgtgatctt ggatgcggga tcggggctgt
      241 tttcggccca atgggtcagg gtgcagcggc aggtctcctc tgccacacgc gtgtgcgcct
      301 acgaccgggc ggggatgggc tggagtgaga tgggccccgg tcccagggac gccaagcaga
      361 tcaccagcga actgcacacc ctgctttcca aagccggcat cgaaggccct tacgtgctgg
      421 tgggccactc cttcggcggc atgtacatgc aaacgtacgc cgcccgctat cccgacgagg
      481 tggcgggcgt ggtcttggtc gattcctcta ccgatccgga tcagttcagc cagcgaccgg
      541 aagcacagga tagcaacgaa ccacaaaagc agacatccgc cgtggtttcc cagctggtgc
      601 ggttcggcat ctccctgcct gcgcgggtcg ggattgtccg cttgttgtcc tatctcgatc
      661 gggcttcccc cgagttgccg caggagcagc gcgcgcaaac cgatgcactc accccctcga
      721 cgcgagaatt cactacctcc gtactagagt ttcttgcccc cacccagacg ctcagactgg
      781 ggagtccggg gagcctggga aacaaaccgc tggcggtggt cactgccccc aaacaggcgg
      841 agcccggttg gatcaagcat caggacaaac tggccaccct ctcgaccaac agcatccacc
      901 gggtcgtgaa gggggcgacg cacgaatcac tgttgtacga tcggagcgac gcgcaatcga
      961 ctagcgctgc catcgttgag gtggtgggcg tcgtgcgaaa cgatcggccg ctggcgcaag
     1021 aaaaccacta acctcctgca cctgaacttt ttagaacgcc gttagggcga agttcgcaga
     1081 atttaccttc atcgaaggtg ggtgaatagg ggaagtggcg catcctatcg agccggggcg
     1141 ctccccctac caccgaatgt gggcgctacg gggccgtagc gcagagcgcg ctggccctca
     1201 ccaaccaatc tgcactggtc ggattctcct cgaacgcggt tacgtcaacg gactgggtgg
     1261 ctgtcgagag ctccacatcg gtgaccacga cgtcggtctg tagctggagg aaggagccac
     1321 ttgtgccgcc gatgagttcg tagccgcccg acaccaagac tttctcgtcc ggacaatccg
     1381 cccgcagggt cttggaggag tcggagttgg tggagctgtc ctcttcgacc agctccaagc
     1441 cgttgactcc tatttcctcg gagtccttgc cgtcgagctt gtcggcgttt aggttggtcg
     1501 ccttgcccgc gacggcgttc acgtttatgg gcggcctccc gtcctccacc ctcagatcca
     1561 acgcccgcga tccggatgcg gaggagtcgt tgtccaccgt cagcatggcg cccccgtccc
     1621 tggaacccgc tagtttggtg agggcgttat tgatggtgtt ggtttgcccc agattgaaag
     1681 ggtcacccgg gaccgcggca atagccacgg tcccagcccc aagcatcaac gctatagtca
     1741 ataccgctcc ccagaagatg gccgccccct tggccaacca catcatcctt ctcatcagcg
     1801 ctctcgctcg catgctcttc gctcctgttc tctgtagttg cctacctgac gaggatcagc
     1861 atagggtgca ggcaagggag ggcgcatgcg tgaaactacg caacgggttc gctatttatg
     1921 gggaggaggc taagggcaga gacagcagcc gaagttcttg gaacgccaat tgtcggccat
     1981 ctaagtgcgg gacggagaag tccgctctgg atggccacca ccgacgaggg cgcagatggc
     2041 gagctctgtt ttcgctgact tcgcggttct tgaccggtac cctctactgg gaacttagtc
     2101 ttctcctggt cgcgcgctcg gcgcgcgctc tcaagctcgt cgacgatgtt cattatcatc
     2161 cgcttctcgg gacccggcag gcggaagctc ttgcgcgcta tggcgctcgt cgtctcgtca
     2221 cgaagtagtc caatgcctcc ctgtcgagca aaggtggttc ctctaaccca tccaggaagt
     2281 accccgcctc gacgcccaag actctggcga gcgcgaccag cttgtccacg gtagggctcg
     2341 ggatcatccc cgcccttatg cccgccacct cgtcctcggt aaggacgcct aagctcatgc
     2401 gagccacctc ggcgtcggtg taaggcgatc cagtcttgtc gttggtgatc gtgttcagta
     2461 gtagatcgac cttgtcggcc aagttctggc ctttctccgc cgagtcggct tggattccca
     2521 gaccgagttc ctcgaaccac agccccggcg ggaagcccat tgctttagct atggccgcca
     2581 tcttctcata accaggattt tcgatcctgt ctttacggag attggttata tacgagcgtg
     2641 ttacgatccc gttggaggca tcctggaggt cctgctgtcc gtgagcgtct tgcccttgtg
     2701 gcagaggacg gcctttggcc ggccggcgcc gggatcacac gctctgtcct ctcgcgctgg
     2761 taaggttatt gctgtcccga gtacagggta ggcatccgcc aggtcaggca catctgtcag
     2821 gtggctactt ctggggagga actttctgaa acaccacaca cgaagtcccc agaacgggct
     2881 ttcccgcggg aggtgggcgc ccgtggccgg gtggatggta cgatcatcct cgtgatgcgg
     2941 gacgattcgc cagaggatta cgtccgctac ccgagacatc gcaggttcgc atgaccgggg
     3001 acccagtcta cacgacccgc gacctcggcc tggatacctg ggacgacttc gccaggctgg
     3061 tcgaggcgaa caacggcgtc tggggtgggt gctggtgcat gggcttccat cccgagggac
     3121 tcgaccggga ggacgcctca gccaaccgcg atgccaaacg ggcccacgtc gagcggggga
     3181 ccgttcacca ggtgctcgtc tacgcggacg aggactgcgt gggctggtgc cagtacggga
     3241 ccgccgcgga gctgcccaac atccagaacc gcaaggccta cgcaaaggac ctcgtcgtct
     3301 tgccggcgtg gcgcatcggc tgcatcttca ccgacaggtc gcaccggggt cagggggtcg
     3361 cgcgtgccgc cgtaggcgcc gccctggcgg cgatcgaggc ttccggcggg ggcatcgtcg
     3421 aggcctaccc ggagcagatc gagggacggc cgccgcagcg cgggtcctac cttcacaccg
     3481 gccccgagac cctgttcgag gagttcggct tcagaaggga ccgaaggatc tccaagtggc
     3541 gctgggtgat gcgtctgagg gtggatgccc catcacgacc cggttagggt ggggtcaccg
     3601 tactggatcg cgtataagcg gggaacgcca ggctataggt ttcaggtatc gggttttctg
     3661 aggcccgagt cctgcgtggc tcatttcccg gttctcgtag gggtcccgtc tgcgcgctgt
     3721 ctttcgatgg cgtcgacggt tttgatccac cgtcgtatgg cgtcggcgtc gtggagcgag
     3781 acgccggtac tgttcgccag cgcgcgggca ccccgggtgt atccggcggg ggcgactacc
     3841 caggcttcga cgcagcggtg gtggcgcgcc ccggcgtaga cttcctgcac gggcctgttg
     3901 ccgacgggca cggcatggtt cttgcactgt acggctatgc gccctttccc gaggtcgatg
     3961 atgatgtcga ctccctggtc acctggcccc ccgcggggct ggacgcgatg ccccatggcc
     4021 ctgaaaaggt cggccatgaa ggcctcgaag cgggccccgc ccatggaacg gaccgactcg
     4081 aagcaccgcg agagctccag ggcggtcggg gtggtgggcg tgttccagag atgcctggac
     4141 gccaacgcta cggcggcgag ggccaccacc agaagggtgc cgccgaagag gatcttggcg
     4201 ccacggatca acccgacgtc gccacgcgac acgagtgcga gcaatgccag gcacgcgatg
     4261 accccaccgt acaggatcac atcgggtagc ctgtttcccg acccgctcat acgtccagcc
     4321 tgccccgatc cgcccttcgc gctagctcgc ctcgaatcgt gcgggtagat ggccgacccc
     4381 caaaggaacg gtatcccgca gcccccggga acgctcatgg ccgccggttt cccga
//
LOCUS       DBNGVD010000281         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2798109, whole genome shotgun sequence.
ACCESSION   DBNGVD010000281 DBNGVD010000000
VERSION     DBNGVD010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2798109"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16CM_07615"
     CDS             <1..652
                     /locus_tag="AC16CM_07615"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3139735.1"
                     /translation="ELRSKEYAHDYRYFPEPDLLPLELSPAYVREIEARLPELPEHML
                     SRFVEQYGLSEYDAGVLTSDRDLAAFYEAVAAEVDPKQASNWVSGDLRALLNETATDI
                     SDSKVEPAHMTELIELVAAGSVSRSAARTALDRVFETGDSPSEVVEREGLASVGSDEL
                     SGVVDEAISSNPDEAERVRNGDKKVIGFLVGQVMKATRGNADGGRVRELLMEKLGS"
     gene            697..1911
                     /locus_tag="AC16CM_07620"
     CDS             697..1911
                     /locus_tag="AC16CM_07620"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF051391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3139736.1"
                     /translation="MNVPRVGVPFFESSDLADKVSEAGGEPVLLRLPAARPGTGVALA
                     REWMADSLEVQCSARDLDGLLLHAECPEEYFGMLVAALRLDLPTVCARPASLPLFAAL
                     VGLGLVSGVNGPATTSVSLVERDGPRSGELVDNFSLANALRSGVAAGGGPELLVHLAA
                     LAREAGVTGFDQMIRVLIPETSEVPPEWLVEHGVPALLSSLGDVLHDVPTVTGNLLEN
                     LPTAPPPPEAHANFVFVKARASGAEAVCRMREGVTEAAGKCRVFGSEKEAVSAVREGG
                     VGGGTMLVVGGCGPRGGQGLLRLDGLGLALREAELGVPVLTDGLAPESATGTWISLFT
                     PEATMGGVIGLLRDGDPLRIDLTQGRIRAEIGAGELETREPTGFPDPPETAYAARYAR
                     SALPALEGAGFG"
BASE COUNT          294 a          672 c          687 g          312 t
ORIGIN      
        1 cgagctccgc agcaaggagt acgcccacga ctaccgctat ttcccggagc cggacctgct
       61 ccccctggag ctctcgccgg cgtacgtgcg ggagatagaa gcccgactcc ccgagttgcc
      121 cgaacacatg ctgtcccgat tcgtcgagca gtacggcctc tccgagtatg acgccggcgt
      181 gctgacgtcc gacagggacc tcgcggcgtt ctacgaggct gtcgcggcgg aggttgaccc
      241 caaacaggcc tctaactggg tctccggcga cctgcgggct ctcctcaacg agacggcgac
      301 ggacatctcc gactcgaaag tggagccggc acacatgacg gagcttatcg agcttgtcgc
      361 cgccggaagt gtctcccgct cggcggccag gacggccctc gacagggtct tcgagaccgg
      421 tgactcgcca tcggaggtcg tcgagcgcga gggcctcgcc tcggtcggct cggacgagct
      481 ctccggtgtc gtggacgagg ccatctcgtc caaccccgac gaagccgaac gtgtccgcaa
      541 cggcgacaag aaggtcatcg gctttctcgt ggggcaggtg atgaaggcga cccgcggcaa
      601 cgccgacggt ggacgcgtgc gcgagctgtt gatggagaag ctgggctcct agggttcggg
      661 catcaggctg caggcatcag caaccggtgg tcggccctga acgtaccccg cgtcggcgtt
      721 ccctttttcg agtcctcgga cctcgccgac aaggtcagcg aagccggcgg cgaacccgtg
      781 ctcttgcgtt tgcccgcagc ccggcccggc accggggtcg ccctggccag ggagtggatg
      841 gcagattccc tggaggtcca gtgctcggcc agggacctag acggcctgct gctccacgcg
      901 gaatgccccg aggagtattt cgggatgcta gttgcggccc tgcggcttga cctccctacg
      961 gtgtgcgcgc ggcctgcgag cctcccgctc ttcgccgccc tcgtcgggct tggcctcgta
     1021 tcgggcgtca acgggcccgc gaccacgtcc gtctccctgg tcgagcgcga cgggccgcgt
     1081 tccggcgagc tggtagacaa cttctcgctg gccaacgcgc tcaggtcggg ggtcgcggcc
     1141 ggcggcggcc ccgaactcct cgtccacctc gcggcgctcg cccgcgaggc aggtgttacg
     1201 gggttcgacc agatgatccg ggtcctcatc cccgagacgt ccgaggtacc cccagagtgg
     1261 ctcgtggaac acggcgtacc cgccctgctc tcctcgctgg gcgacgtgct acacgacgtc
     1321 cccaccgtta cgggcaacct tctggagaac ctcccgaccg cgccgcctcc cccggaggcg
     1381 cacgcaaact tcgtcttcgt gaaggccagg gcgtcggggg ccgaagccgt ctgccgcatg
     1441 cgcgagggtg tcacggaggc ggcggggaag tgccgcgtct tcggctccga gaaggaggcg
     1501 gtctcggccg tgcgcgaagg cggggtcggg ggaggcacga tgctcgttgt gggtggttgc
     1561 gggccgcgtg ggggacaggg gctgctgagg ctcgacggtc tggggctggc cctgcgcgag
     1621 gccgaactcg gggtgccggt gctcacggat gggctcgcgc ccgaaagcgc aacggggacc
     1681 tggatctcgc tctttactcc ggaggccacg atgggcggcg tcatcggcct tctccgcgat
     1741 ggtgatcccc tccgaataga cctcacccag ggccgcatcc gcgcggagat cggggcagga
     1801 gagctggaga ccagggaacc aaccgggttc ccagacccac ccgagacggc gtacgccgcc
     1861 cgctacgcca ggagcgcgct acccgcgctg gagggcgccg gcttcggcta acctccagcg
     1921 gtggccggct ttctggccct gacgtgcagg aagtaggcca cgacc
//
LOCUS       DBNGVD010000282         5104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2980981, whole genome shotgun sequence.
ACCESSION   DBNGVD010000282 DBNGVD010000000
VERSION     DBNGVD010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2980981"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /locus_tag="AC16CM_07625"
     CDS             <1..1795
                     /locus_tag="AC16CM_07625"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR3139737.1"
                     /translation="GAVLATSLWYGLFILAFLLCAMAALSQMNLSEARERAGLETAPS
                     KGLLLGSVVPCFFAVMLVGLVLFSFLPQRHAMSLTMMPTSAFQNISDRFSGGVQNPYY
                     RPPGGDPFSGPPMSISPSSYHGFLPYMDLRSRGRLSDEIVMKVRSEETVPYRGVVFDD
                     YNGKGWEISTGEDAERLDSDGVRFDTYTAQNAEPAQGPYREVAQIFNVEEDSSNVIFG
                     AYHPETVYFPTSSIEIDPYGALRAPYEIPSGSTYSVISHLPNATPTQLRSAGTAYPEE
                     IASRYTSLPPTGLGRTSALADRLTEGTKNPYDAVLKMNEYLKRTYPYDLSIPPQSEDM
                     NAVEYFLFEQKRGYCEQFSSSLAVMARSQGIPARVATGYAPGEYNPFTGLYDVRASDA
                     HAWVEVYFPGYGWSTFDPTPSFDSTPWQYRAADNLQGGKAFTFLASRLGEVAGPAAGA
                     AGKLMCGVARLDPASIIVVGLLFAGAYLLVFYGGKRYLRSRRQPPSVRPIKVSDARLY
                     SRYRRVTTALEEAEITRAPQETPESYARRAAESLDEESIARLGEIYLYARFRDAVPST
                     LVEEFDRLEPRALSAIERAISRQPSTTGHQA"
     gene            1994..2959
                     /locus_tag="AC16CM_07630"
     CDS             1994..2959
                     /locus_tag="AC16CM_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139738.1"
                     /translation="MGEQNGGPTLEGLAQRLEALTEKLGGLERENEALRKELSTRGGP
                     GAHRSGDGEPGSLLQGMMSRRSLLSKSGAAAVAAVAAGTLLYPREASANHFGPGIEVD
                     WVFAHSDDRSAVTGVSGPAGIYGVYGRAERPGYFGVAGRNQTAEGGTGVDGYSPNGVG
                     VKGRGNNGVHGEANSTGWAGVYGNNIVLGYGCVGDGSGSSNAGVLGRGTGGSDGVRGE
                     SAGTGADVAGVKGKNTGSGAGIMGEASSSGSAGVYGRNPNGYGGRFEGQGKAHLQLIP
                     RDTVGPPTAGGHGKGELVMDRNATLWVCVVGGGPGTWRRFQTAAT"
     gene            3115..3687
                     /locus_tag="AC16CM_07635"
     CDS             3115..3687
                     /locus_tag="AC16CM_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139739.1"
                     /translation="METAVDERPDRLFFEEAQRFSLWIRVIVLVPVFALGLPACVMWF
                     GDDGGPGLVGVLFTVLALFALVGAALTFMMRLDTRLDSGNLRLWIHPRRWSLLPGRMT
                     RKDIAVSDISRWRVRTYNALSGTEYWGWHVWGLGVARGGRFLYMMRPDNPIRGRGVQL
                     QLASGERVLVGSGRPEELEHAITTAKNASR"
     gene            4162..4551
                     /locus_tag="AC16CM_07640"
     CDS             4162..4551
                     /locus_tag="AC16CM_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3139740.1"
                     /translation="MEINKDLVAASSTPMVLAILAEEDSYGYAILQRVRESSGGHMEW
                     TDGMLYPVLHRLERLGHIEARWEVAQSGRRRKYYRITSRGRAQLAEERRQWQAVDATL
                     RGIWQAFVPSLVSARHPAPALAPPGDA"
     gene            4554..>5104
                     /locus_tag="AC16CM_07645"
     CDS             4554..>5104
                     /locus_tag="AC16CM_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3139741.1"
                     /translation="MSEPGEGVSLEEQIGQWRGYLRHRRAIHPVDVAELEDHLREQVA
                     VLVEAGLTTDEAFLVAVKRMGKLDALSREFAREHSDRLWKQLVVVPSDPEDARPRVRT
                     DALVAFCVAIAAAVLIKLPALFGIQLGDEEEAGFYLRNLSLFVLPLLSGYFAWKRRLD
                     ASTIRLLVLAFVLAGVFANVYPFV"
BASE COUNT          895 a         1656 c         1703 g          850 t
ORIGIN      
        1 tggcgccgtc ctcgcgacga gcctgtggta cggcctcttc atcctggcct tcctgctgtg
       61 cgccatggcc gccctctccc agatgaacct ctccgaagcc cgcgagcggg ccgggctcga
      121 gacggcaccg agcaagggtc tcctgctcgg ctcggtcgtg ccctgcttct ttgccgtgat
      181 gctggtcggg ctggtcctct tctccttcct tccccagcgg cacgcgatga gcctgacaat
      241 gatgccgacc tcggcgttcc agaacatcag cgaccggttc tcgggcggcg tccagaaccc
      301 gtactacagg ccgccgggtg gggacccgtt ctccggaccg cccatgtcca tctccccgtc
      361 gtcctaccac ggcttcctgc cctacatgga cctgcgcagc cgcgggaggc tctccgacga
      421 gatcgttatg aaggtgagga gcgaggagac cgtaccctac cgtggggtcg tcttcgacga
      481 ctacaacggc aaggggtggg agatctccac cggcgaagac gccgagaggc tggactcgga
      541 cggcgtgcgc ttcgacacgt acacggccca gaacgccgag cctgcgcagg gaccatacag
      601 ggaggtggcc cagatcttca acgtcgagga ggactcctcg aacgtgatct ttggcgccta
      661 ccaccccgag acggtctact tcccgacgag cagcatcgag atcgacccct acggcgccct
      721 gagggcaccc tacgagatcc cctctggctc tacctactcc gtgatctcgc acctcccgaa
      781 cgccacccca acgcagctcc gctccgccgg aacggcctac ccggaagaga tagcgagcag
      841 atacacaagc ctccccccca ccggcctcgg acgcacgagc gccctcgccg acaggctgac
      901 cgagggcacg aaaaacccct acgacgccgt gctgaagatg aacgagtacc tgaagaggac
      961 ctacccctac gacctgtcca tcccacccca gagcgaggac atgaacgccg tggagtactt
     1021 cctcttcgag cagaagcggg gctactgcga gcagttcagc tcctctctgg ccgtgatggc
     1081 ccgctcgcag ggcatcccgg cgagggtggc cacgggctac gcacccggcg agtacaaccc
     1141 cttcaccggc ctctacgacg tgcgcgcctc cgacgcccac gcctgggtcg aggtctactt
     1201 ccccggatac ggctggtcga ctttcgatcc caccccgagc ttcgactcta cgccctggca
     1261 gtacagggcg gcggacaacc tgcagggcgg caaggccttc accttcctcg ccagcaggct
     1321 cggtgaggta gcaggtccgg cggcgggcgc ggccggcaag ctgatgtgcg gcgtggcccg
     1381 cctcgacccg gccagcatca tcgtggtggg cctgctcttc gccggagcct acctcctcgt
     1441 cttctacggc ggcaagcgct acctccgctc acgccgacag cccccgtccg tacgccccat
     1501 caaggtctcg gacgcccgcc tctacagcag atacaggagg gtaacgaccg ccctcgaaga
     1561 ggcggagata acccgggccc cgcaagagac ccccgaaagt tacgctcgca gggccgccga
     1621 atcactagac gaggaatcca tagcccgcct gggcgagata tacctctacg cccgcttccg
     1681 agacgccgtc ccgtcgaccc tggtggaaga gttcgaccgc ctcgaaccac gagccctctc
     1741 agcgatagag cgggcgatca gccgccagcc gtcaactacg ggccaccagg cgtagagacc
     1801 tccccgtagg ggcggttcgc gaaccgccca tcgaacgtgc cgacacccga aggaccggaa
     1861 caaagcccca cgattcagag accgctacga aaaaacccga aatgatcttt tcgtaacgtt
     1921 gttcgtaacg ttcaggcgac gctctctcgc tatggtacgg gcacacaaag cgaggcggaa
     1981 agagagggcg agcatgggtg aacagaacgg tgggccgacc ctcgagggcc tggcccagag
     2041 gctcgaggcg ctgaccgaga agctgggagg gctggagcgc gagaacgaag cactccgaaa
     2101 agagttgtcc acacggggtg ggcctggtgc gcaccgctcc ggagacgggg agcccggatc
     2161 tttgctccag gggatgatga gcaggaggtc tctgctcagc aagtccgggg ccgcagcggt
     2221 ggccgccgtg gccgcgggaa cgctgctgta cccgcgcgaa gccagtgcca accatttcgg
     2281 ccccggcatc gaggtggact gggtgtttgc gcatagtgac gacaggagcg ctgttacggg
     2341 ggtctctggc ccagcgggca tctacggtgt atacggtagg gcagagaggc ccgggtactt
     2401 tggtgtggcc ggccgcaacc agaccgccga aggcggcacc ggcgtcgacg gctacagccc
     2461 gaacggggtc ggcgtgaagg gccgcggcaa caacggggtc cacggcgagg ccaactcgac
     2521 cggctgggca ggcgtgtacg ggaacaacat cgtccttggc tacggctgcg tgggggacgg
     2581 gagcggcagc tccaacgccg gcgtgctcgg acgtggcacg gggggctcgg acggcgtcag
     2641 gggagagtcc gccggtaccg gcgccgacgt cgccggcgtg aaaggcaaga ataccggatc
     2701 cggcgcaggc atcatgggtg aagcgagcag ctccgggtca gccggagtgt acggacgcaa
     2761 cccgaacggt tacggcggga ggttcgaggg ccagggcaag gcgcacctgc aattgatacc
     2821 aagagacacc gtcggtcccc ccaccgccgg cggtcacggc aagggcgagc tggtcatgga
     2881 cagaaacgcg acgctctggg tctgcgtggt cggcggtggt ccaggcacgt ggaggaggtt
     2941 ccagacggcc gccacctgag ggtcttgctg tatcgcctcg cgagggccgg aggttcgcgc
     3001 cccggccctc caggggcgga ttagctatta gctatcagct ttcagctttt ggcggtgaga
     3061 agccgccggc ttttggcgac ggctggtggc tatgtctggg gaggaactgt tcgtatggag
     3121 actgctgtgg acgaacgtcc ggatcggctc tttttcgagg aggcgcaacg cttctctttg
     3181 tggataaggg ttatcgtgct ggtgccggtc tttgcgctcg gtcttccggc ttgcgtgatg
     3241 tggtttggcg atgatggggg tcccggtctc gtcggggtgt tgttcacggt gctcgccctg
     3301 ttcgccctcg ttggtgcggc gctgaccttt atgatgcggc tcgacaccag gctcgactcg
     3361 ggcaacctca ggctctggat ccacccacgc aggtggagct tgctgcccgg acgaatgacg
     3421 cggaaggaca tagccgtcag cgacatctcg cggtggcggg tgcgtaccta caatgcgctg
     3481 tcgggtacgg aatactgggg gtggcacgtc tggggccttg gcgtcgcgag agggggcagg
     3541 ttcctctaca tgatgaggcc agacaacccc ataagaggac gcggggtgca actccagctg
     3601 gcgagcggcg agagggtgct cgttggatcg gggcgccccg aagagctgga gcacgctatt
     3661 accacggcca agaacgcaag ccggtagccg ccggatacga cggaaccagg aaggtcaggg
     3721 gcaggcggag gcacccacgc gcggatgcca ggggcacggt cctcgaagcc agggctacac
     3781 agcgcttcga gccgtgcaag tcctccggga gcgggaaccc ggcccggcag gcgcccaccc
     3841 tcggcaaaac cggccacccg agaagatgcc gaatctggtt gtatggtctc ctagtctgtt
     3901 tgtgggccaa catgccgcac cttctccctt tccccgtcat tcccgcgaaa gcgggaatcc
     3961 acgcccgccg aagacgtggg tctctgcgtt ctgggggcaa gatccacccg ggttacgtat
     4021 gatgccttgc cgccttcgcc ccgctcgaca ctccccgaac ccccgccctt tatttacctg
     4081 gcgctgctat gtataatgct gtcaggtata tacggacgag atcgccagcg ttgcgggacg
     4141 cgtggggtcg tagggaggat tatggagatc aacaaggatc tggtagccgc atcttcgacc
     4201 cctatggtgc ttgcgatcct ggccgaggag gacagctacg gttacgctat cctccaacgc
     4261 gtacgggagt cgtcgggggg gcatatggag tggacggacg ggatgctcta ccctgttctg
     4321 caccggctcg agcggctcgg ccacatcgag gcgcggtggg aggtcgcgca gagcgggcgc
     4381 cggcgcaagt actaccgtat cacctcccgg gggcgggccc agctcgctga ggagcgcagg
     4441 cagtggcagg ctgtggacgc cacgctacgg ggcatctggc aggcgttcgt cccgtcccta
     4501 gtctcggccc gccacccggc gccggccctg gcacctcctg gggatgcctg acgatgtcgg
     4561 agccgggtga gggggtatct ctagaggagc agatcgggca gtggcggggc tacctccgcc
     4621 accggcgggc gatccacccc gtcgacgtgg ccgagctgga agaccatctg cgcgaacagg
     4681 tagcggttct ggtcgaggcg gggctaacca ccgacgaggc gttccttgtg gcggtcaagc
     4741 gcatgggcaa gctcgacgcg ctctcgcggg agttcgcgcg cgagcattcg gaccgactct
     4801 ggaagcagct cgtcgtcgtt ccctcagatc ccgaggacgc gaggccccgg gtcaggacgg
     4861 atgccctcgt cgccttctgt gttgcgattg cggcggccgt gctcatcaag ctgccggcgc
     4921 tatttgggat ccagctgggt gacgaagagg aggccggctt ctacctccgc aacctgagcc
     4981 tcttcgtgct gcccctcctg agcggctatt tcgcctggaa acggcggctc gatgccagca
     5041 ccattcgcct gctggtcctc gctttcgtct tggccggcgt gttcgccaac gtctacccgt
     5101 tcgt
//
LOCUS       DBNGVD010000283         5030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1887499, whole genome shotgun sequence.
ACCESSION   DBNGVD010000283 DBNGVD010000000
VERSION     DBNGVD010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1887499"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="AC16CM_07650"
     CDS             complement(<1..1143)
                     /locus_tag="AC16CM_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3139742.1"
                     /translation="MDLYGYRRENGSVGIRNHVVILPVDDISNAACEAVADHIKGTMA
                     LPHSYGRLQYGEDLALHFRTMIGIGANPNVAAVVVIGIEENWTKKIADGIAKTGKPVA
                     SFSIEGKGDLETVRRASWKAKEFVQWASETRREPVELGELTASIKCGESDTTTGLASC
                     PTVGQAVDRLVDEGATVFFGETSELTGGEHLIAERVATPELKEAFQKTYDKYVALIET
                     QSEDLLGSQPTQGNIAGGLSTIEEKALGNIEKTGTKEIIGVLRPAERPENGPGLYFMD
                     SSSAAAEHVTLMAAGGAVVHMFPTGQGNVVGNPIVPVIKVSGNPNTVATMGEHIDVDV
                     SGLLSREITLPDAGNLMIEYLVRTANGRLTSAEALGHREFTMTKLYP"
     gene            complement(1146..1424)
                     /locus_tag="AC16CM_07655"
     CDS             complement(1146..1424)
                     /locus_tag="AC16CM_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3139743.1"
                     /translation="MHKFLIHKKDDHVGVATSDIGPGEKVVGVYMDDDSLVEVTSKGE
                     VPLGHKIAVEGCEPGGAVVEYGVQIGKAPEGFAVGDYVHTHNLKSARW"
     gene            1718..3211
                     /locus_tag="AC16CM_07660"
     CDS             1718..3211
                     /locus_tag="AC16CM_07660"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3139744.1"
                     /translation="MGVSANEVTRSILIGGEWRATETYCEVTNPATGEVVSRQARSDE
                     DVVREAINSAAGAHEEWQKTSGFERYQFLWRVGDLLSERAEAIGETLAEETGKLLAEA
                     VGEVRFSADYFYWFAGEARRLEQLSVVDGRATGPQVVVKKPVGIVASLTPWNFPVSIQ
                     ARKVAPALAAGCTVIARPSEEAPNSVVQLFRCLVDAGLPEGAANLVTGSAGAVTNPMM
                     EDPRVRLVSFTGSTGVGKMLVEKSAPTMKKLALELGGCAPFIVLEDADLDHVLEQAMI
                     AKLRNCGQSCVAANVYYVHDDLYEKFVEGLADRVRALKLGDPFDEQTTVGPLINAERR
                     RAIVELRARAVSAGFEPVASAPSLDGGSALSDDCFFAPELLAAPSHKKVEDELLRTEI
                     FGPLVLVAGFSDLGRLIPKLNQNPLGLAGYVFSRDTAKAARVATRLEVGISGINEGVA
                     SAANVPMGGVKDSGLGREGGHVGMEEFLEPQYLAVRGKSLSAWAFGL"
     gene            3264..4673
                     /locus_tag="AC16CM_07665"
     CDS             3264..4673
                     /locus_tag="AC16CM_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139745.1"
                     /translation="METVTIRSIRDVYDFVNTRPMASARTRILVFIALGGIFIDAYDL
                     TSLGIGVDSLQEQLSLTPFQLGSVTAIMALGALVGALVGGILADKYGRYKMVVVNLLF
                     LVFAAIGAALAPNLTWLLVFRFLLGVGVGMDLPLALTFIAEFTNSRIKGAAVNLWQLV
                     WYVAAVCTGLVALPFLLIGVEENLWRYAVGFAAVPALLVLILRLIYVDESPMWAAQHQ
                     GLEEAGKILEKNYNVRIVVAPPEEQVEEVVPRNERPRLREIFSPQLRMRTVLASIISG
                     TQSMEYFAVGFYLPTIAVLIFGEGALFAVVGAIALNLFGIVGGGTQVFLTQRLGVWNL
                     AVIGYCIAAASLIVVGLTRDSIPAYAGALLIGLFIFGHSFGPGSQGKTMAALSYPTNL
                     RGTGTGWAETMSRVGSILGFYVFPLVLATAGLYNTLLYLAVVPIIGLTTLLLIRWEPV
                     GKDVEETQESPAQAAVSSG"
BASE COUNT          913 a         1589 c         1537 g          991 t
ORIGIN      
        1 agggtaaagc ttggtcatcg tgaactcccg gtggcccaaa gcctccgcgg atgtgagacg
       61 cccgttcgcc gtgcgcacca ggtactcaat catcaggttg ccggcatcgg ggagcgtgat
      121 ctcgcggctc aacaacccgc tcacgtccac gtcgatgtgc tcccccatcg tcgcgaccgt
      181 gttcggattc ccggacacct tgatgaccgg cacgatgggg ttgccaacga cgtttccctg
      241 accggtcggg aacatatgca ccacagcccc gccggcggcc atcaacgtta cgtgctcggc
      301 ggccgccgag gagctatcca tgaaatagag cccgggtccg ttctccggcc tctccgccgg
      361 cctgagtacc ccgatgatct ctttcgtccc cgtcttctcg atgttgccga gcgccttctc
      421 ctctatcgtc gagaggccgc ccgcgatgtt cccctgcgtc ggctgcgagc cgagcaggtc
      481 ctcggactgc gtctcgatca gggctacata cttatcgtag gtcttctgaa atgcttcttt
      541 gagctccggg gtcgccaccc tctcggcgat taggtgctcc ccgccagtaa gttccgaggt
      601 ctcaccgaaa aagacggtcg cgccctcgtc cactagccga tccacggcct gcccgaccgt
      661 cgggcaggag gcgagacccg tcgtggtgtc cgactcgccg cacttgatgc tggccgttag
      721 ctcaccgagt tcgaccggtt ctcggcgcgt ctccgaggcc cactgtacga actcttttgc
      781 cttccacgaa gcccgcctga cggtttccag atccccctta ccttctatgg agaaggaggc
      841 caccggcttg cccgtcttgg ctatgccgtc ggctatcttt ttcgtccagt tctcctcgat
      901 gccgatgacg accaccgcgg cgacgttcgg gttcgcgccg atcccgatca tggtccgaaa
      961 atggagcgca agatcctccc cgtactgcag cctgccgtaa gaatgcggta gggccatcgt
     1021 gccctttata tggtccgcga ccgcctcgca agcagcgttt gagatgtcgt cgaccgggag
     1081 tatcacgacg tgatttcgga tgccgactga gccattctcg cgcctgtagc cgtagagatc
     1141 catgtctacc acctcgcgct cttcaggttg tgcgtgtgga cgtaatcgcc tacggcgaac
     1201 ccctcgggag ccttgccaat ctggactccg tattcgacca ccgcaccgcc cggctcgcac
     1261 ccctccacgg ctatcttgtg tcccaaagga acctctccct tcgaggtcac ctcgaccaag
     1321 gaatcgtcgt ccatgtacac tccaacaacc ttctcccccg gccctatgtc ggaggtggcc
     1381 accccgacgt ggtcatcctt cttgtggatc aagaacttgt gcatctctac tcccccgctt
     1441 ataggtgtta ccaattcaca caaaaatgct accaaatacc caaatgaggc gcaaggttcc
     1501 gtgggggatt ttcgtcggca gagattagca aacatgcaaa ctcacccttc gtgagccggt
     1561 aatttgcggc aatctaccta accgagaacc ccacaacgcg aacgtacgag cgtctggcac
     1621 cgcgacaaag aagttatcga ttgctattga aaggtattac caatttggta taacctacca
     1681 ccaattccag ggaaagcggt gagaggggaa gagtctgatg ggcgtaagcg cgaacgaggt
     1741 gacgagatcc atactcatcg gcggcgagtg gcgggcgacc gagacgtatt gcgaggtgac
     1801 gaaccccgcc acgggcgagg ttgtttcgcg tcaggcgaga tccgacgagg acgtggtgag
     1861 ggaagccata aactctgctg cgggggctca tgaggaatgg cagaagacct cgggcttcga
     1921 gcggtatcag tttctctggc gggttgggga tctgctctcc gagagggccg aggccatcgg
     1981 tgagacgctg gccgaagaga cgggcaagct gctcgcggag gccgtcggag aagtgcggtt
     2041 ctcggccgat tatttctact ggtttgcggg ggaggcgcgg cggttggagc agctttcggt
     2101 cgtggacggg agggcgaccg gtccgcaggt cgtggtcaag aagccggtag gcatcgtcgc
     2161 cagcctcacg ccctggaact tcccggtctc gatccaggcc cgcaaggtcg cgcccgccct
     2221 cgccgccggc tgcaccgtga tcgcccggcc cagcgaggag gccccgaact ccgtcgtcca
     2281 gctttttcgg tgtctcgtgg atgcagggct gcccgagggc gctgcgaacc tcgttaccgg
     2341 aagcgccggt gccgtcacga atccgatgat ggaggatccc cgcgtcaggt tggtaagctt
     2401 caccggctcg accggcgttg ggaagatgct ggtcgagaag tccgctccca cgatgaagaa
     2461 gctcgctctg gagctgggcg ggtgcgcccc cttcatcgtg ctggaggatg cggatctgga
     2521 ccacgtcctg gagcaggcca tgatcgccaa gctccgcaat tgtgggcagt cgtgcgtcgc
     2581 ggccaacgtt tactacgttc acgacgacct ctacgaaaag ttcgtggaag gactcgcgga
     2641 tcgagtgcgt gccctgaagc tcggggatcc gtttgacgaa cagaccacgg tagggccgct
     2701 catcaacgcc gagcgtcgca gggccatagt cgagctgcgg gcgcgggctg tctccgctgg
     2761 gttcgagcca gtagcctcag ccccgtccct ggacggtggc tcggcgctct ccgacgactg
     2821 cttcttcgcc cccgagctgc tcgcggctcc ctcacacaaa aaagtcgaag acgagttgct
     2881 ccggacggag atcttcggcc ccctcgtcct ggtcgccgga ttctcggacc tcggtcgcct
     2941 gattcccaaa ctgaaccaga accccctggg gctcgcgggc tacgtgtttt ccagggacac
     3001 ggcgaaagcg gcacgggtgg caacacgatt ggaggtgggc atcagcggca tcaacgaagg
     3061 ggtggccagc gccgcaaacg tccccatggg cggcgtcaag gacagcggcc tcgggcgtga
     3121 agggggccac gtcggcatgg aagagttcct cgagccccag tacctcgccg tgaggggcaa
     3181 gtccctgtcg gcatgggctt tcgggctata ggagaggaga aaagcacgtg gaaagaaacg
     3241 gagcagacgg gaggaacacg cctatggaga ccgtgaccat ccgttcgata cgggatgttt
     3301 acgatttcgt caacacccga cctatggcca gcgcgaggac gcgaattctg gtgttcatcg
     3361 cgctcggcgg catctttata gacgcctacg acctgacgag cctgggcatc ggggtggact
     3421 ccctccagga gcagctctct ctcacgcctt tccaactggg cagcgtcacc gcgatcatgg
     3481 ctttaggcgc cctggtggga gccttggtgg gcggcatcct cgcggacaag tacggtcgct
     3541 acaagatggt cgtggtcaac ctgctctttc tcgtcttcgc cgccataggc gccgcgctcg
     3601 ccccgaacct gacgtggttg ctcgtgttcc gtttcctact cggtgtgggg gtcggtatgg
     3661 atctgccgct ggcccttacc ttcatcgccg agttcaccaa ctcgcggata aagggcgcgg
     3721 ccgtcaacct ctggcagttg gtctggtacg tggccgccgt ttgcacgggc ctcgtcgctc
     3781 taccgttcct cctcataggt gtggaagaga acctgtggcg ctacgcggtc ggcttcgccg
     3841 ccgtaccggc gctactcgtc ctaatactcc gcctcatcta cgtggacgag agcccgatgt
     3901 gggccgccca gcaccagggg ctcgaagagg cgggcaaaat cctcgaaaaa aactacaacg
     3961 tgcggatagt cgtcgcccct cccgaggagc aggtcgagga ggtcgtcccg cgcaacgaga
     4021 ggccgcgcct gcgcgagatc ttctcccccc aactcagaat gaggaccgtc ctcgcctcca
     4081 tcatctccgg cacccaaagc atggagtact tcgccgtcgg cttctacttg ccgaccatcg
     4141 ccgtcctgat ctttggagaa ggcgccctgt tcgcggtcgt cggggcgata gcgctgaacc
     4201 tcttcggcat cgtgggcggc gggacccagg ttttcctcac gcaaaggctc ggggtgtgga
     4261 atctcgccgt catcgggtac tgcatcgccg cggcgagcct catcgtcgtc gggctgaccc
     4321 gcgacagcat tccggcgtac gcaggagcgc tcttgatagg actcttcatc ttcgggcatt
     4381 ccttcggccc cggttcgcag ggcaagacga tggccgccct ctcctacccg actaatctca
     4441 ggggtacggg tacaggatgg gcagagacca tgtcccgggt cggtagcatc ctcggcttct
     4501 acgtgttccc gctggtcctg gccaccgcag gcctctacaa cacgctcctc tacctggcgg
     4561 tcgtgccgat tattggcctg acaaccctcc ttttgattcg ctgggagccg gtgggcaagg
     4621 acgtcgagga gacacaggaa tctcctgccc aagcggcggt gtcctctgga tagacgtcca
     4681 tcgaaccttg ccgtaggaga cctacccgct cttccgtgcg tccgagaccg cttccacgag
     4741 atgccgcggc caccatgatc cttcagcgag tgcgtgcttt gcgcttgagt gcattggcat
     4801 gaaagagatt ctcgcaggag gccacgtcgg catggaagag tccctcgatg cccggtacct
     4861 cgtcgtgagg ggtaaaccct tcgcgacaga ggcccttgga ggacaagaaa gtggacaaag
     4921 acgccgatga cgagggtgcc cgcaggaccg atagcgccgc cgcgcagcgg tagggcgcgg
     4981 ggtcgatgag agtgtatcta gcaaaggagc accactgctg tgagcgatac
//
LOCUS       DBNGVD010000284         3243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2798158, whole genome shotgun sequence.
ACCESSION   DBNGVD010000284 DBNGVD010000000
VERSION     DBNGVD010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2798158"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..704
                     /locus_tag="AC16CM_07670"
     CDS             168..704
                     /locus_tag="AC16CM_07670"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3139746.1"
                     /translation="MANDQEGDHVLKQAVQDAMNEQIKNELFSAYQYLSMAAYCESES
                     LPGFAHWMRAQAQEETVHAMKFYDFILERNGRVVLGPIDGPLVEFGSPLEVFQSALEH
                     EQKVTGMINDLYGLAVRENDYASQAFLQWFVTEQVEEEKNAGDVVETLRMIGDGTEAL
                     FLLDRELGQRRVEEPAAG"
     gene            885..1313
                     /locus_tag="AC16CM_07675"
     CDS             885..1313
                     /locus_tag="AC16CM_07675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR3139747.1"
                     /translation="MAERMSRDEYYEFLLGRARTAVLCTVRADGRPHAAPVWFDLDGD
                     AFVITTGQDTVKGRNIRRDPHVALCIDDENPPFHFVIVEGTAVLSVQDPDLLHWSTRI
                     GGRYMGPEKADEYGRRNAVPSELLVRISPQKVLAQKNISD"
     gene            1370..2059
                     /locus_tag="AC16CM_07680"
     CDS             1370..2059
                     /locus_tag="AC16CM_07680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3139748.1"
                     /translation="MDGSRADTDFGGAGLVERLREAVAQPFEGWREVYESFSPVDLET
                     GERRPRVPPPGANSRRAAVLVPILLDDGEARVVYTVRKGDLAEHAGQISFPGGSAEPS
                     DASLMETALREAEEEIDLSRGLVEVIGELEEMYIPPSDFRVSPFVGLLPARAELVLAP
                     DEVEEIFTVSLETLTAPETLRKVPWTYEGCDYEVPVFATGGPSPRNIWGATAAMTGVL
                     LARLGWHRYRR"
     gene            2067..2468
                     /locus_tag="AC16CM_07685"
     CDS             2067..2468
                     /locus_tag="AC16CM_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139749.1"
                     /translation="MLRLVAIAIFGLMFLAEAGDIFGLALTLADPQPAADRSGLAVGA
                     EVVRHSVLLIFAVVLAGSALLAVVGLLARKPALYHRGALVCAVGYLVYGVFQVAGGAL
                     QVGAGTVVVAGLIYMMLGGIAYAMNRSVSPG"
     gene            complement(2455..2781)
                     /locus_tag="AC16CM_07690"
     CDS             complement(2455..2781)
                     /locus_tag="AC16CM_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139750.1"
                     /translation="MSDEKYPCTKDLRSTFTSTTGGVLTNEVGAITGQLELLTRPARS
                     GIEAEVRYEGARDLYTVSGSPLETTDSHPEGHRTILQKLTTPGRIERGNELPVDLTSS
                     QGVSPG"
     gene            complement(2778..>3243)
                     /locus_tag="AC16CM_07695"
     CDS             complement(2778..>3243)
                     /locus_tag="AC16CM_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019151.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HIR3139751.1"
                     /translation="ADGTRFGWDEPDGSCDVRDDALIRDGRGVEVDEDCSITAGEWLD
                     PFTGATLADSQDVDIDQFVPLANAWRSGADGWDAEQRETYANDPGVLLSVDDAANQTK
                     GDKGPEAWLPPNSDYRCEYARRWIWIKHEWNLTVNEQERTTLQDLLDGCETG"
BASE COUNT          541 a          983 c         1104 g          615 t
ORIGIN      
        1 cgggatcact cacctggggg gagtgggttg gcggctcgat actggccgcg ctggtcatgc
       61 ccgtggcggc cttcaccacc tcgctggccc actccaacct cgagaagcgt tcttgaatcg
      121 ggccttcatc ccaccggaag cgatcggggt atagtacggt cagagatgtg gcgaacgacc
      181 aggaaggaga tcacgtgctc aagcaggcgg tccaggacgc gatgaacgaa cagataaaga
      241 acgagctatt ttcggcctat cagtatctgt cgatggccgc ctactgcgag tccgaaagcc
      301 tgcctggctt tgcgcactgg atgcgcgccc aggcgcagga ggagaccgta cacgccatga
      361 agttctacga cttcatcctg gagcgtaacg ggcgcgtggt gttgggccct atcgacgggc
      421 cgctggtcga gttcgggtcg ccgctcgagg tgtttcagag cgctctggaa cacgagcaga
      481 aggtcacggg gatgatcaac gacctctacg gcctggcggt gagggagaac gattacgcga
      541 gccaggcttt cctccaatgg ttcgtaacgg agcaggtaga ggaggagaag aacgccggtg
      601 acgtggtcga gacgctcaga atgatcgggg acggcaccga agcgctcttc ctgctcgaca
      661 gggaactggg gcagcgccgt gtggaggagc cggcagccgg gtgaaggacc tcgcgaccaa
      721 ccgcccacgg tccgtcgtgc tcgctcttca gagaagcgac caggtggctt cttatggatg
      781 atagctgacg gcagatagcc cgcttcccgc agcctcgggc gcttcgaaaa ccctcgagat
      841 gtctagaggg tgaccgggta agctggtgat ggaggtgtgt ggatttggcg gaacgaatgt
      901 cccgggacga atactacgag tttctgctcg gtagggcgcg cacggcggta ctctgcacgg
      961 tccgggccga tggtaggccg cacgcggctc ctgtctggtt cgacctcgac ggcgacgcgt
     1021 tcgtcatcac gacgggacag gacacggtca agggccgaaa catacgccgg gatccccacg
     1081 tcgccctctg catcgacgac gagaacccgc cgtttcactt cgttatcgtc gaaggtacgg
     1141 cggtgctatc cgtccaggac cctgacctcc tgcactggtc cacccgcatc ggcggtcgct
     1201 acatgggtcc ggagaaagcc gacgaatacg gcaggcgcaa cgccgtgcca tcggaactac
     1261 tggttcgcat aagcccccag aaagtactgg cccagaagaa catctcggat tgagcgccgc
     1321 ctcctggcaa gggggccgaa ccggagccga agcgtgctag cattgcgtga tggacggatc
     1381 tcgggcggat actgattttg gcggtgctgg cctcgtggag cggttgcggg aggcggtcgc
     1441 ccaacccttc gaagggtggc gtgaggtcta cgagagtttc tcgccggtgg acctggaaac
     1501 gggagagagg cgcccacggg tgccgccgcc gggggcaaac tcgcggcggg cggcggtgct
     1561 ggttccgatc ctcctggacg acggggaggc cagggtggtg tatacggtga ggaagggaga
     1621 cctcgccgag catgccggcc agatctcctt ccccgggggc agcgccgagc cgtcggacgc
     1681 atcgcttatg gagacggcgc tacgtgaggc cgaggaggag atcgacctca gccgcggcct
     1741 ggtcgaggtt atcggagagc tggaggagat gtacattcca ccctctgatt tccgcgtgag
     1801 cccattcgtc gggctgctgc cggcccgagc cgagcttgtg ctcgcgcccg acgaggttga
     1861 ggagatattc acggtctccc tggagacgct caccgccccc gagacgcttc gcaaggtgcc
     1921 ctggacgtac gaaggctgcg actacgaggt gccggtcttc gctacagggg ggccgtcacc
     1981 gcgcaacata tggggcgcta cggcggcgat gaccggggtc cttctcgcac gtcttggctg
     2041 gcaccgttac aggcgctagg ggcggtgtgc tgcggctcgt cgccatagcc atcttcggtc
     2101 tgatgtttct ggccgaggca ggggatatat tcgggctggc gcttacgctg gccgaccccc
     2161 aaccagccgc tgaccggtca ggtctggcgg ttggggccga ggtggtacgg cacagcgtcc
     2221 tcctgatctt cgccgtggtt ctagccggca gcgccttgct ggccgtggtc gggctgctcg
     2281 cccgcaagcc agcgctctac catcggggag ccctggtttg cgccgtaggc tacctggtgt
     2341 acggggtgtt ccaggtcgct ggcggggcgc tccaggtcgg tgccggcacc gtcgtcgtgg
     2401 caggcctgat ctacatgatg cttggcggca tcgcctacgc gatgaaccgc tccgtcagcc
     2461 cgggctgacc ccctggctgc tggtcaggtc gactggaagc tcgtttcccc tttcgatcct
     2521 gcccggggtg gttagcttct ggagtatggt ccggtggccc tccgggtggg agtcggtggt
     2581 ctccagaggt gagccgctca cggtgtacag atctcgggcg ccctcgtaac ggacctcagc
     2641 ctcgatgccg ctgcgcgcgg ggcgggtgag tagctccagt tggccggtga tggctcccac
     2701 ctcgttcgtt agcacgcctc cagtggtgct ggtgaaggtg gacctcaggt ccttcgtaca
     2761 gggatatttc tcgtcgctca ccctgtttcg caaccatcga gcaggtcctg cagcgtcgtc
     2821 cgctcttgct cgtttaccgt caggttccac tcatgcttga tccagatcca acgccgcgcg
     2881 tactcgcaac ggtaatcgga gttgggcggc agccacgcct cggggccttt gtctcccttg
     2941 gtctgattgg cggcgtcgtc gacggaaagc aatactccag ggtcgttggc gtatgtttcc
     3001 cgctgctccg cgtcccaccc gtccgcgccg gatcgccagg cgttggcaag gggaacgaat
     3061 tggtctatgt cgacgtcctg cgagtccgca agcgtggcgc cggtgaaggg gtccaaccac
     3121 tcaccggcgg taatggagca atcctcatcg acctcgaccc cgcgtccgtc cctgatgagg
     3181 gcgtcgtctc gaacgtcgca acttccgtcg ggctcgtccc acccgaaccg ggttccgtca
     3241 gcg
//
LOCUS       DBNGVD010000285         5964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_611712, whole genome shotgun sequence.
ACCESSION   DBNGVD010000285 DBNGVD010000000
VERSION     DBNGVD010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_611712"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="AC16CM_07700"
     CDS             <1..884
                     /locus_tag="AC16CM_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3139752.1"
                     /translation="AARKITQGAGTRVAVLDTGAQLTHPALKANFAGVPRYDFVDDDT
                     DPSEPTLSKNKERRAQEVVGHGTHVAGIVDLVAPRAEIMPLRVLDRAGYGTTFHIAEA
                     IAYAEANGADVINLSLGTPSWSRLLRDKVDAAIGSGVVVVAAAGNHGSLQPQYPASNG
                     PPPLSVARETDGLLAVTSVDAAERKSDFANWGKWVDIVAPGEQILSTYPVSRYAYWSG
                     TSMASPFVAGEAALIQSADGPLVPGGVEDRIRSGARCVNQKNELKYTGMLGSGHADVD
                     ASLRGIEGPHCTSLVGL"
     gene            983..1471
                     /locus_tag="AC16CM_07705"
     CDS             983..1471
                     /locus_tag="AC16CM_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3139753.1"
                     /translation="MEAWRTLLKKYERLVFSVPRKYGLSAEDAADVTQLTFTILVQSM
                     DKLPEDSRLGGWLTTVARRHTWRLLERNRRQGIGRHAVLDENIMGGGTNPIESWELGE
                     WLGQGLSLIGRSCRDLLFALYLDPTQPSYAEVAERLGMAVGSVGPTRIRCLERLRRVI
                     DE"
     gene            1582..2211
                     /locus_tag="AC16CM_07710"
     CDS             1582..2211
                     /locus_tag="AC16CM_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139754.1"
                     /translation="MPRDDRQIGFDLLADWLEGRLPADEARSVEEAVAAADGDTLADV
                     AWLRKFFEATESAIVESPPPWLEDTLVATFEDHTRGRPAPGLFRRMLAGLTFDSNLQP
                     AAGLRAVGGQQARRQLIYQAEVFDLALNFMARDADSDLDIDGQAFPHEDGEQEILVVQ
                     LLRDGEELALTVTDELGSFAFRRVEPGRYELVLGTEGTEVSISPVDVSL"
     gene            2215..5097
                     /locus_tag="AC16CM_07715"
     CDS             2215..5097
                     /locus_tag="AC16CM_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3139755.1"
                     /translation="MTSGAGGLILPGPVEGLLSGSVPAEEGAAVLRAANLLDTEGLGR
                     LLDMAEELVHSDPGKAHRLAELCATGAAAGGLPAIAARSAYVRLQTHFARGEFDAALR
                     MAREAYEGYLASDMALDAVRTHVGRMSVLLELGLYQEALDAGQTVLDTLDGRGDVTVY
                     PSTHQRDMLTALVQQNLGGCLEYMGRYEEALAAYSVAESLYGELGETERVGEILDNRG
                     AVLLYLGRGTEALGAHEAAARVFAGANLTLPYATALCNIGEANRQLADYRSSLIAFEE
                     AHRIYQNLDALKDESQLVLDTANVYLDLNLYPEALAAYQRSSNLLRRTGMAHDCARAL
                     WGMGVTLALTSNFEEAERSLSEAAELFSAAGNSTFLSGVMLGQSLVQEKRGDPEASLA
                     HAERALELVLERDWSVQRVYAHLRMVDVLLPDVDGAERHLMQARLLSERLALPQLRQR
                     LNERLGRLRRLQGSEEEALVALEEAVDEIERLRDTVTHESMRASFLLDKIGAYEELLK
                     VHLHRWAESDPRGVFAVAERAKSRGLVDLLTGVSRRGALPADEPLQERIMSLQSDLNA
                     TYNQMLGVGEVPPQVPLPDLHGRTIELEREISELRLRTVPLTSDPFTTAAPPEVWEEL
                     PPDVTLVAYHIMEDEIIAFVGGRDRVRVVRGCGSAGVVAKLLRQLDVQWDRMGADPGF
                     VGKHTEHLTRLTERVLASLYDELVASLEPLPGAPSAGGHDPQKITIIPHGMLHRVPFH
                     ALFDGEAYLLDRFEVSYAPSATVFTLCQRQEPRDQESALVLSVRDPLIPHVSGEALAV
                     ASHLPGAELLADGQATVDAVRERAPGCDVLHLASHGMFRGDNPMFSSLKLHDGWLTAA
                     DVLALDLDGALVTLSACESARNRVLAGDEIVGLARGFLGAGAATLVASLWLVQDETTS
                     WMMEGWYARMREGAGRTTALREAQLALRERHPHPYYWAPFVLMGRR"
     gene            complement(5127..>5964)
                     /locus_tag="AC16CM_07720"
     CDS             complement(5127..>5964)
                     /locus_tag="AC16CM_07720"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3139756.1"
                     /translation="RALRFLGLGTSSLCPVSADGQGRMRPDALRYALAESSEPTIICA
                     QAGNVNTGAIDPLDDICDAAGDAAGDAWVHEDGAFGLWAAASPSLRHLVRGLEHADSW
                     ATDAHKWLNVPYDSGLVFCAHPRSHRAAMGTQASYLVASETGERDQLDWNPEASRRAR
                     GFPIYAAIRSLGRSGVADLVDRCCAHARRFAEALEREPGVEVLADVVLNQVLVRFLDP
                     GGDHGSRTRTVIRAVQEDGTCWLGGTTWQGQVAMRISVSNWSTTTEDVDRSVEAILRA
                     AR"
BASE COUNT         1047 a         1947 c         2044 g          926 t
ORIGIN      
        1 cggcagcccg aaagatcacc caaggggcgg gtacgagggt cgctgtgctc gacaccggcg
       61 ctcaactgac acatcccgcg ctcaaggcaa acttcgccgg cgttccacgc tacgatttcg
      121 tagacgacga cacagatcct tcggagccga cgctctccaa gaacaaggaa cgacgcgcgc
      181 aggaggtggt cgggcacggc acgcacgtgg ccggaatcgt cgatctggtg gccccacggg
      241 ccgagatcat gccgcttcgg gtgctggata gggcgggcta cggcaccacc ttccacatcg
      301 ccgaggcaat agcctacgcc gaggctaacg gggctgatgt gatcaacctg tccctgggca
      361 cgccgagctg gtccaggctt ctccgggaca aggtcgatgc tgctatcggt agcggcgtcg
      421 tcgtggtcgc cgccgccggc aaccacggct cgttgcaacc ccagtacccg gcgtccaacg
      481 gtcctccgcc tctgtcagtc gccagggaga ccgacggctt gctcgcggta acctcggtcg
      541 acgccgcgga gaggaagtcc gacttcgcca actggggaaa gtgggtcgac atcgtcgcgc
      601 cgggtgagca gatcttgagt acctacccgg tgagcaggta cgcgtactgg agcggcacat
      661 cgatggcctc gcccttcgtg gcgggggagg cggcgctgat tcagagcgcg gacggcccgc
      721 tcgtacccgg gggtgtcgag gacaggatac gatccggcgc ccggtgcgtg aaccagaaga
      781 acgagctgaa gtacacgggc atgctgggct ccggccacgc cgacgttgac gccagcctca
      841 gggggatcga aggaccgcac tgtacctccc tggtcggcct gtaaccagcg gcggaccggg
      901 ggtctagatc aactttcccg aagagagggg agcagggggc gaatccgacc gcgacctgat
      961 ccgggcctgc cgcgatggcc gggtggaggc ctggcgaacg ctcctgaaga agtacgagag
     1021 gctagtattc tccgtaccca gaaaatacgg gctctcggcg gaggacgcag ccgacgtcac
     1081 gcagctcacg tttaccatcc tggtgcagag catggacaag ttgccagagg acagcaggct
     1141 tggcgggtgg ctcacgacgg tggcccgccg gcacacctgg cgccttctcg aacgcaaccg
     1201 ccgccagggt atcggcaggc acgcggtgtt agacgagaac atcatgggcg gcggcacgaa
     1261 ccctatcgag agttgggaac tcggcgagtg gctgggccag gggctgtccc tgatcgggcg
     1321 gtcctgcaga gacctcctgt tcgcgctcta cctcgatccc acgcagccct cctacgccga
     1381 ggtcgccgag cggctgggca tggccgtggg gagcgttggc ccaacgagga tacgttgcct
     1441 ggaacgcttg agacgggtga tcgatgagta gcccagagac cagaaaacgg gcaagtcgtg
     1501 gtcccgtgta ttcgattgcc gtgtacgggg catttgttag acaaggggcc ggcaagcgac
     1561 ctcccccgct cgagaggtaa ggtgccacga gacgatagac agataggttt cgatctgcta
     1621 gccgactggc tggagggccg ccttcccgcg gacgaggcca ggtcggtcga ggaggccgtc
     1681 gccgcagccg acggcgatac gctcgccgat gtggcctggc tgcgcaagtt cttcgaagcg
     1741 accgagagcg ccatcgtgga gtcaccaccc ccctggctgg aggacacgct ggtggcgacc
     1801 ttcgaggacc atacgagagg ccggccggca ccgggtctct tcaggcgcat gctcgccggg
     1861 ctgaccttcg acagcaacct gcaacccgcc gctgggctaa gggccgtcgg cggccagcag
     1921 gcacgtcggc agctcatcta ccaggccgag gtgttcgacc tcgccctcaa cttcatggcg
     1981 cgcgacgcgg acagcgacct agacatagac ggtcaggcct tcccccacga ggacggggag
     2041 caggagatcc tggtcgtcca gctactgcgt gacggagagg agctggccct gaccgtcacg
     2101 gacgaactgg ggagcttcgc attcaggcgc gtcgagcctg gccgatacga actcgtgctc
     2161 ggcacggagg gcacggaggt atcgatatcc cccgtagacg tgagcctcta acgcgtgacg
     2221 tccggtgccg gaggcctgat cctccccggc cccgtagagg ggttgctgtc cggatcggtg
     2281 cccgccgaag agggtgccgc cgtgttgcgc gcggcgaact tgctggacac cgaaggactc
     2341 ggtcggctgc tcgacatggc ggaggaactg gtgcacagcg atcccggcaa ggcccatcgc
     2401 ctggccgagc tgtgcgcgac gggggcggcg gctggcggtc tccccgcaat cgccgcccgc
     2461 tcggcctacg tccggctgca gacccatttc gccaggggcg agttcgacgc ggcgcttcgt
     2521 atggccaggg aggcatacga gggatacctg gccagcgata tggcgctcga cgccgtgcgg
     2581 acccacgtgg gcaggatgtc ggtgctcctc gagctcgggc tctaccagga ggcccttgac
     2641 gccgggcaga ccgttctcga cacgctcgac ggccggggag acgtgacggt ctaccccagc
     2701 acccatcagc gagacatgct gacggcgctc gtgcagcaga acctcggggg ttgcctggag
     2761 tacatgggac gctacgaaga ggcgctcgcg gcctacagcg tggccgaaag tctctatggg
     2821 gaactcggcg agacggagcg cgtcggggag atactcgata atcggggcgc cgtgctcctc
     2881 tacctcgggc ggggcaccga ggccctgggc gctcacgaag cagccgcacg ggtcttcgcc
     2941 ggggctaatc ttacgctgcc ttacgcgacg gcactctgca acatcggcga ggccaacagg
     3001 cagctggctg attacaggtc cagccttatc gccttcgagg aggcgcatcg catctaccag
     3061 aacctcgacg cgctcaaaga cgagagccag ctcgtcctcg acacggcaaa cgtctacctc
     3121 gacctgaacc tctaccccga ggcgctcgca gcctaccaga gatccagcaa cctcctgcgc
     3181 aggacaggga tggcccacga ttgcgctcgc gcgctctggg gcatgggcgt tacgctggcc
     3241 ctcacctcca acttcgagga ggcggaacgg tcactttccg aagccgctga gctgttctcg
     3301 gcggcgggaa actccacctt tctgagcggg gtaatgctcg gccagtcact cgtgcaggag
     3361 aagcgcggcg atcccgaagc ctccctggct cacgccgagc gggctctgga gctcgtcctc
     3421 gagagggact ggtccgtgca gcgcgtctac gcccacctgc ggatggttga cgtgctgctg
     3481 cccgacgtgg acggggccga acggcacctc atgcaggcgc gcctgctctc cgagcgcctg
     3541 gcgctacccc agctgcgcca gaggctcaac gagcggctgg gtcggctgag gaggcttcaa
     3601 ggcagcgagg aggaggcgct cgttgccctc gaggaggcgg tagacgagat cgaacgcctg
     3661 cgcgacaccg tgacccacga gtcgatgcgc gcctccttct tgctggacaa gatcggggcc
     3721 tacgaagagc tactcaaggt gcacctccac aggtgggccg agagcgaccc gcgcggggtt
     3781 ttcgccgtgg ccgagcgcgc caaatcccgc ggcctggtgg acctattgac cggcgtctcc
     3841 aggcggggag cactccccgc tgatgagcca ttgcaggaac gcatcatgag cctgcagtcc
     3901 gacctcaacg ctacatacaa tcagatgcta ggcgtcgggg aggtaccgcc gcaggttccc
     3961 cttcccgacc tgcacggtcg gactatcgag ctggagcggg agatcagcga gctccgcctg
     4021 cgcacggtgc cgctcacctc ggaccctttc accacggccg cgccgccgga ggtgtgggag
     4081 gagctccccc ccgacgtgac gttggtcgcc taccacatca tggaggacga gatcatcgct
     4141 ttcgtcgggg gacgggaccg ggtcagggtc gtgcgcggtt gtggatcggc cggcgtggtg
     4201 gcgaagctcc tgcggcaact tgacgtgcag tgggaccgca tgggagctga ccccgggttc
     4261 gtaggcaaac acacggagca cctgacccgg ctcaccgagc gcgtgcttgc ctcgctatac
     4321 gacgagctgg tagcatccct cgaaccgctc cctggggctc catccgcagg cgggcatgat
     4381 ccgcagaaga tcaccataat cccccacggc atgctccaca gggtgccatt tcatgctctg
     4441 ttcgacggcg aggcgtacct gctcgaccgc ttcgaggtct cctacgctcc gagcgccacc
     4501 gtcttcaccc tctgccagag gcaggaaccc cgggaccagg agagcgcgct cgtcctgagc
     4561 gtcagggacc ccctgatccc ccacgtgtcc ggggaggctc tggctgtggc ctcgcatctc
     4621 ccgggagcag agttgctggc cgacgggcag gcgaccgtgg acgccgtcag ggagcgtgcc
     4681 ccgggttgtg acgtgctgca cctggctagc cacggcatgt tccgggggga caaccccatg
     4741 ttctcatcgc tcaagctgca cgacggctgg ctgacggccg ccgacgtgct ggccctcgac
     4801 ctagacggcg ccctcgttac gctgagtgcc tgcgagtcag cgcgcaaccg ggttctggcc
     4861 ggtgacgaga tagtaggcct cgcccgcggt tttctcggcg cgggggccgc gacgctcgtc
     4921 gctagcctgt ggctcgtgca agatgagacc acgtcctgga tgatggaggg ctggtacgcc
     4981 agaatgcgcg agggcgcagg cagaaccacg gcgctgcgcg aagctcagct ggcactcaga
     5041 gagaggcacc cgcatccgta ctactgggcc ccattcgtgc taatgggacg gcggtaggac
     5101 gggcggcaga gcagcgtcag gagccatcag cgggcggcac gcaggattgc ctccacggag
     5161 cgatctacat cttcggtcgt ggtcgaccag ttggagaccg agatcctcat ggccacctgg
     5221 ccctgccagg tcgtgccccc aagccagcag gtaccatcct cctgcacagc cctgatgacg
     5281 gtacgggtcc tggagccgtg gtcaccgccg gggtcgagaa agcgtacgag gacctggttt
     5341 agaaccacgt cggcgagcac ctcgacccca ggctcccgct caagcgcctc ggcgaagcgc
     5401 cgggcgtgag cacagcagcg gtcgacgagg tcggcgacac ccgaacggcc caacgagcgg
     5461 atggccgcgt agatcggaaa cccgcgcgcc cggcgtgagg cctcggggtt ccagtcgagc
     5521 tggtcccgct cgccggtttc ggaggcgacc aggtagctcg cctgcgtccc catggccgcc
     5581 cggtgcgacc gggggtgcgc acagaagacg agccccgaat cgtaggggac gttcagccac
     5641 ttgtgggcgt cggtggccca ggaatccgcg tgctccaggc cccggacgag atgccggagg
     5701 gatgggctgg cggcggccca caaaccgaag gccccatcct cgtgcaccca ggcgtcgccg
     5761 gccgcgtcgc cggccgcgtc gcagatgtcg tctaggggat ctatggctcc cgtgttcacg
     5821 tttccggcct gggcgcagat gatggtgggt tccgaagact cggccagggc gtaacgaaga
     5881 gcatctggcc gcatccggcc ctgtccgtcc gctgatacgg ggcacaagct cgaggtgccg
     5941 aggcccagaa agcggagtgc ccgg
//
LOCUS       DBNGVD010000286         4125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4621214, whole genome shotgun sequence.
ACCESSION   DBNGVD010000286 DBNGVD010000000
VERSION     DBNGVD010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4621214"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16CM_07725"
     CDS             <1..254
                     /locus_tag="AC16CM_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3139757.1"
                     /translation="SLAGEWAPRGVRVNSVSPGYIRTPLTELGMSKTEWSEVWLSQTP
                     MGRLAEPEEIAPAVLYLASDAATFATGTNLVVDGGYTSW"
     gene            406..1659
                     /locus_tag="AC16CM_07730"
     CDS             406..1659
                     /locus_tag="AC16CM_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3139758.1"
                     /translation="MGENRAGASRERVGEVYRSESRRVFATLVRLLGDFDLAEEALHN
                     AFAAALEQWPRDGVPGNPRAWLVSVGRFKDIDALRRRARFDAALTELADRLDEGVGEP
                     DIAEEDGLEDDRLRLIFTCCHPALSSEAQVALTLREVCGLTTEDVARAFLIPAPTLAQ
                     RIVRAKARIRDEQIPYRVPIREELPGRLDGVLRVVYLVFNEGYSASAGDALMRPDLSG
                     EAIRLGRVLVALLPEPEAVGLLALMLLQDSRRKARTTPDGDLVPLEEQDRGLWDRRRI
                     TEGISLVRRALSQDGFGPYTIQAAIAAVHAQAPSVTATDWAQIVGLYDLLLRITPSPV
                     VELNRAVAVAMLDGPEDGLLLVDAIMDRGELEGYHLSHAVRADLCRRMGDVVAARGSY
                     KRALELAEQEPERRFLARRLADLAR"
     gene            1777..2169
                     /locus_tag="AC16CM_07735"
     CDS             1777..2169
                     /locus_tag="AC16CM_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3139759.1"
                     /translation="MRFLVMHKSNEENEAGVPPSPALVENMGKLMEESARAGVLLAAE
                     GVHPSSKGARLSFSGGRRTVTDGPFAEAKELIAGFAVLQVDSKEEAIEWATRFADVIG
                     DVEIEVRQVVELSGFASEAGTTNEGGQQ"
     gene            2166..2720
                     /locus_tag="AC16CM_07740"
     CDS             2166..2720
                     /locus_tag="AC16CM_07740"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3139760.1"
                     /translation="MRKVVAAEFLSLDGVMESPDQWHFPYFNDEMGQAVGEGFESSDA
                     LLMGRVLYEEWAAFWPNQDLEENPMATTMNNLTKYVVSTTLEEPLEWNNTSLIGGDVA
                     EEIHQLKQQPGKDITISGSGALVRSLLDYGMLDELKLMVHPIVVGSGKKLFEDGGQRT
                     PLELVQSTTFATGVLYLTYKPADG"
     gene            2813..3607
                     /locus_tag="AC16CM_07745"
     CDS             2813..3607
                     /locus_tag="AC16CM_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3139761.1"
                     /translation="MNSVISRDGTRIAYDELGGGPPVILVGGAFNTRTFGPNKGLAPL
                     LEEHFTVINYDRRGRGGSTDTAPYAVEREIEDLQALIEVSGGSAHVFGISSGAALALE
                     AANSGLAIEKLALYEAPFIVDDSRAPVPDDYLPKLEGMLAADRRNAAIRLFLRQGVGV
                     PAVFVAMMRMMPVWSKLKAVAPTIIYDATLVNDYRRGEPLPSDRWASLTLPTLVAVGG
                     KSPEWMRNAMRELADVLDAEHRTLEGQTHIVKADALAPVLVEFFAE"
     gene            3652..4023
                     /locus_tag="AC16CM_07750"
     CDS             3652..4023
                     /locus_tag="AC16CM_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139762.1"
                     /translation="MNIETSGQIDPGRARIVDVSDPGYGDDTNEVQGHNHNVAVSIIV
                     VDAPPGGGPKLHKHPYEEVFVIQEGTATFTAGEETIKAKAGKVVIVPSGIPHKFVNTG
                     EGRLRQVDIHASDRFITEWLE"
BASE COUNT          805 a         1308 c         1330 g          682 t
ORIGIN      
        1 ggagcctcgc cggggagtgg gccccacgag gcgtacgggt caacagcgtc tcgcccggtt
       61 acataagaac gcccctgacc gagctcggca tgtccaagac cgagtggtcc gaggtgtggc
      121 tctcgcaaac ccccatggga cgcctcgccg aaccggagga gatcgccccg gccgtcctct
      181 acctcgcctc cgatgcggca accttcgcca ctggaacgaa cctcgtcgtc gacggcggct
      241 atacctcgtg gtaagcaagt ggtcaacggc gtcaccgaga cgcaagcgaa cgtccctgta
      301 ggggcggttc gcgaaccgcc cgactgcgtg ccgcccaggg gaataacccg ccgttgcccc
      361 cacgacgcca cccgctaaca tactggtatg tcccgtacgt ccgatatggg ggagaacagg
      421 gccggcgcct cgcgtgagag ggtgggtgag gtctatcgct ccgagtctcg acgcgtcttc
      481 gccaccctgg ttcgcctgct tggcgacttc gacctcgccg aggaggcgtt gcacaacgcc
      541 ttcgccgccg cgctggagca gtggccgagg gacggcgtgc ccggtaatcc gcgcgcgtgg
      601 ctggtctccg tcggccgctt caaggacatc gacgctctgc gccgccgcgc ccgcttcgac
      661 gcggccctca cggaactcgc cgaccggttg gacgaagggg tcggagagcc tgatatcgcg
      721 gaagaagatg gactggagga cgacaggctg cgcctgatct tcacctgttg ccatcccgcc
      781 ctctcttccg aagcgcaggt ggccctgacg ctgcgcgagg tctgcggtct gaccacagaa
      841 gacgtagccc gagccttcct gatccccgcg cccacgctgg cccaacgcat agtacgcgcc
      901 aaggccagga ttcgtgacga gcagattcct taccgcgtgc ccattcggga ggagctaccc
      961 gggcgtctgg acggtgtgtt acgcgtcgtc tacctcgttt tcaacgaggg gtactccgcg
     1021 tcggcgggtg atgcgctcat gcggcccgac ctctcgggag aggccatccg gctcgggcgc
     1081 gtgctggtcg ctctgctgcc cgaaccagaa gccgtgggcc tcctcgcgct catgctgctg
     1141 caagattcgc gccgcaaggc ccgcaccaca ccagatggag acctggtccc cctcgaggaa
     1201 caggaccgcg ggttgtggga ccgtcgacgg ataacggagg gtatctctct ggtcaggcgg
     1261 gccctgtcgc aggacgggtt cggtccttac accatccagg ccgcgatcgc cgcggtacac
     1321 gcgcaggcgc cgagcgtcac ggccacggac tgggcccaga tcgttggact ctacgacttg
     1381 ctcttgcgga taacaccttc gcccgttgtc gagctgaacc gcgccgtggc cgtggccatg
     1441 cttgacggac cagaggacgg gctcctcctc gttgacgcca taatggatcg cggtgagcta
     1501 gagggctacc acctgtccca cgccgtgagg gccgatctgt gccgcaggat gggcgacgtg
     1561 gtggcggcca ggggctccta caaacgggcg ctagagctgg ccgagcaaga acccgagcgg
     1621 cgcttcctgg ccagaaggct cgccgatctg gcgcgttaga gttggccgtc cgaatctttc
     1681 gagaattttc tcgactttct tcgaacctct gtcgattcgg gtgtctgccg aacgactact
     1741 ttgtagaagg cacaaacgac gagaggagag aacaggatgc ggtttctggt gatgcacaag
     1801 agcaacgagg agaacgaggc aggggttccg ccgagcccgg cgctcgtgga gaacatgggc
     1861 aagctaatgg aggagtcggc gcgcgcgggg gtgctgctcg cggccgaggg ggttcacccg
     1921 agttcgaagg gagcgcgcct ctccttctcc ggcggccggc gcacggttac cgacggccct
     1981 ttcgccgagg caaaggagct gatcgccgga ttcgccgttc tgcaggtcga ctccaaggaa
     2041 gaggccatcg agtgggccac ccggttcgcg gacgtgatcg gcgacgtcga gatagaggtg
     2101 cgtcaggtgg tcgagctctc gggctttgcc tccgaggcgg gcacgaccaa cgaaggaggg
     2161 caacaatgag gaaagtggtg gcggctgagt ttctgtccct ggacggtgta atggagtcgc
     2221 ccgaccagtg gcacttccct tacttcaacg acgagatggg tcaggctgtg ggggagggct
     2281 tcgagtcgtc ggacgccctg ctcatgggac gcgttctcta cgaggaatgg gcggcgttct
     2341 ggcccaacca ggacctcgag gaaaacccca tggccacgac gatgaacaac ctaaccaagt
     2401 acgtggtctc gaccaccctg gaagagcccc tggagtggaa caatacatct ctgattgggg
     2461 gcgacgttgc ggaggagatc caccagctca agcagcagcc cggcaaggac atcacgatct
     2521 caggcagcgg tgctctggtc cgctcgctgc tcgattacgg catgctcgac gaactgaagc
     2581 tcatggttca ccctatagtc gtgggtagcg gcaagaagct cttcgaggac ggcggacagc
     2641 gtacgccgct ggagctcgtg cagtccacga ccttcgctac cggcgtcctc tacctgacct
     2701 acaagcccgc cgacggctga gaaaaccgca acacaggccg acgtcgcggg gagataatcc
     2761 ccgtgacggg gcaacgtggc agcaaatatt cgacaacgcg caggtgatca gcatgaactc
     2821 ggtaatctct agagacggta cccgcatcgc ttacgacgag ctgggagggg gaccgccggt
     2881 cattctggtt ggtggagcgt tcaacaccag aacgttcggc ccgaacaagg gcctggctcc
     2941 attgctggag gaacacttca cggtgatcaa ctacgatcgc cggggccggg gcggtagcac
     3001 agacaccgca ccctacgccg tggagcgtga gatcgaagac ctccaagccc tcatcgaggt
     3061 ttcgggcgga tcagcccacg tgttcggcat ctcctcggga gcggccctcg ccctcgaggc
     3121 ggcgaacagc gggctcgcta tcgagaagct agccctgtac gaggccccct tcatcgtgga
     3181 cgatagccgt gcccccgtgc cggacgacta tctgccgaaa ctagagggta tgttggccgc
     3241 ggatcgcagg aacgctgcga tcaggctgtt cttgaggcag ggcgtgggcg tccccgccgt
     3301 gttcgtggcc atgatgcgga tgatgcccgt ctggtcgaag ctcaaagccg ttgcccccac
     3361 gataatctac gacgccaccc tcgtgaacga ttaccgaaga ggtgaaccat tgccctcaga
     3421 caggtgggcc tcccttacct tgcccaccct cgtagcggtc ggcgggaaga gccctgaatg
     3481 gatgcgaaac gcgatgcgcg agctcgcaga tgttctagac gccgagcacc gcacccttga
     3541 aggccagaca catatagtga aggccgacgc tctcgcgccc gtactcgtgg agttcttcgc
     3601 ggagtagccg agcaccaggt atgcggtacg ggcgatacga gaggagaaga cgtgaacatc
     3661 gagactagcg ggcagatcga cccgggcagg gcgcgcatcg ttgacgtatc cgacccggga
     3721 tacggagacg acacgaatga ggtgcaggga cacaaccaca acgtcgccgt ctctatcatc
     3781 gtggtcgatg cgccgccggg aggaggaccg aaactccaca agcacccata cgaggaagtc
     3841 ttcgtgatcc aggaaggcac cgccacgttc acggccggag aggagaccat aaaggcgaaa
     3901 gccggcaagg tcgtaattgt cccaagtggc atcccgcaca agttcgtcaa caccggcgag
     3961 ggccgcctcc gtcaggtcga catccacgcc agcgaccgat tcatcaccga atggttagaa
     4021 tgacccccca catggaacga cgaacccaac ccctgtaggg gcggttcgcg aaccgcccca
     4081 ccacaaaccg cccgcgcaca cgattcgccc gcccgcgacc cccta
//
LOCUS       DBNGVD010000287         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4802417, whole genome shotgun sequence.
ACCESSION   DBNGVD010000287 DBNGVD010000000
VERSION     DBNGVD010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4802417"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>1974)
                     /locus_tag="AC16CM_07755"
     CDS             complement(109..>1974)
                     /locus_tag="AC16CM_07755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3139763.1"
                     /translation="LFLRRPADRMALFVSLALLVFGTATFTDTMDVLAAEPSPWRLPD
                     MMLQAAGTIAFGLFLYLFPDGRFVPRWTAPVALAWVLWQLSSYFATPFTGSDGKGPWT
                     WVVLAVWIVALGTAVYAQGYRYRRVSGAVERQQIKWVVLGISAALGGFLGTTVALAVF
                     APAPDSPKVMLAHLVGHAFSYAAMLLIPISIGIAILRYRLFDVDLIINRTIVYAVLTT
                     GVIGVYVLVVGSMGAVMRVQEDFLVSILAAGLVAVLFQPVRERLQRAVNHFMYGKRDD
                     PYAVLSHLGQRLESTLAPDEVLPAVTRTVREALKLPYAEVRLRRREGFEIAAAAGEPV
                     DDTLRLPLVYGGETVGLLVLGLRAGEESFSSEEERLFADLAHQIGVAARATLLGDEAI
                     RLSSDLQRSRERLVSAREEERRRLRRDLHDGLGPQLASLTMTAEAARDLISTDPRRAE
                     DLLASVMERAQEAVTDVRRLVYELRPPALDALGLLAALRAYADHHTDGGVRVTVESPG
                     ALPPLPAAVEVAAYRIAMEALNNVVRHSGASNCEIRLSLDESAGLLALRVSDDGRGIE
                     GDHEPGVGLSSMRERAEELGGSCAIEAPPSGGTRVTALLPCALDGVYRTGSEGGG"
BASE COUNT          368 a          657 c          655 g          294 t
ORIGIN      
        1 gcctcaccta tcacctcgag gtcggaggcg gagaggagca gggcgcgaag cccgtcacgg
       61 aagtgaggat ggtcgtcggc gatgagcacc cggatctgct ccaaggctct agccccctcc
      121 ttctgaaccg gtccggtaca cgccatcgag cgcgcagggc aacaatgccg taacccgtgt
      181 accgccagaa ggtggggctt cgatcgcaca cgagccacca agctcttctg ccctctcgcg
      241 catcgaggag aggccaacgc ccggctcgtg atcgccctcg atcccccgcc catcatcgga
      301 tactctgagg gccaacaaac cggcactttc gtccagagag agacgaattt cgcagttgct
      361 ggcacccgag tgtcggacga cgttgttcag ggcttccatg gcgatgcggt aggccgcgac
      421 ctcgacggcg gcgggaagcg gtggcagggc gcccggagat tcgaccgtca cccgcacacc
      481 gccgtctgtg tgatggtcgg cgtaagcccg tagcgccgcc agcaggccga gggcatcgag
      541 cgcggggggt ctcaactcgt agacgagcct gcgcacgtca gtgaccgcct cctgggctcg
      601 ttccatcacg ctggcgagca ggtcttcagc gcgtcgcggg tcggtcgaga tcagatccct
      661 cgcagcctcc gccgtcatcg tcaggctggc cagttgtggg ccgagcccgt cgtggagatc
      721 gcgcctgagc ctgcgccgct cttcctcgcg cgcgctcacc agccgctcgc gcgatctctg
      781 gaggtccgag gagaggcgta tcgcctcgtc gccaaggagg gtggcgcgcg ccgcgacgcc
      841 tatctggtgt gccagatccg cgaagaggcg ctcttcctca gacgagaagc tctcctcccc
      901 ggcgcgcagc cccaggacca gcaggcccac ggtctctccc ccgtagacaa gcggcaggcg
      961 gagggtgtcg tctacgggtt cccccgcggc cgccgcgatc tcgaaccctt ccctgcgccg
     1021 cagccttacc tccgcgtagg gtagcttcaa ggcctcccgc acggtcctgg ttacggctgg
     1081 taggacctca tcgggggcga gcgtcgattc gaggcgctgt cccaggtgcg agagaaccgc
     1141 gtaagggtcg tcccgcttcc catacatgaa gtgatttacg gcgcgctgca gcctctcgcg
     1201 cacgggctgg aagagcacgg cgacgaggcc agcggcgagg atggagacca ggaagtcttc
     1261 ctgcacccgc atcacggctc ccatgctacc caccaccagc acgtaaaccc cgatgacgcc
     1321 cgtggtgagg acagcgtaga cgatggtgcg gttgatgatc agatctacgt cgaagagcct
     1381 gtagcgcagg atagcgatgc cgatggagat cgggatgagg agcatcgccg cgtaggagaa
     1441 cgcgtgccct acaaggtggg cgagcatgac tttcggagag tccggcgccg gggcgaagac
     1501 cgccagggcc acggtcgtcc cgagaaaccc gccaagcgcc gccgagatgc ccaagaccac
     1561 ccatttgatc tgctgtcgct ctaccgcgcc cgagacgcgg cggtaacggt agccctgggc
     1621 gtagacggcc gtgccaaggg ccacgatcca gacggcgagc accacccagg tccacggacc
     1681 ttttccgtct gaaccggtaa acggggtagc aaagtaggag gacagttgcc acagcaccca
     1741 cgcgagcgcc acgggcgccg tccaacgcgg tacgaaccgt ccgtcgggga agagatacag
     1801 gaagaggccg aaggctatcg ttcccgcggc ctggagcatc atgtcgggca gccgccaggg
     1861 agagggctca gcggccagca cgtccatggt gtccgtgaag gtcgccgtcc cgaagacgag
     1921 cagggccagc gatacgaaca gcgccatacg gtcggcgggc ctgcgcaaga acag
//
LOCUS       DBNGVD010000288         4934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3527547, whole genome shotgun sequence.
ACCESSION   DBNGVD010000288 DBNGVD010000000
VERSION     DBNGVD010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3527547"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="AC16CM_07760"
     CDS             <1..1109
                     /locus_tag="AC16CM_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR3139764.1"
                     /translation="RLGGNAEGRRSGIGLKATPGALSGEQIAFLVRQRVARLATADAA
                     GRPHVVPVCFAFDQSSVYIALDEKPKKVPASDLKRVRNILANPEVALTADRYAEDWSL
                     LAFLMVQGVAEILEPDTDEHAAAVRLLRGKYHQYETMRIQENPVIAIRPERAVAWGAL
                     DEPGSHGPGVLDTIQGRRSVRKYLPKEVTDGVVEQVLEAARWAPSPHGRQPWRFAVIR
                     RDETRERLADAMGEEWRANLEMDGQSQEIVERRLEGSRRRLLDAPVLVLLCLYLEDLD
                     EYPDAGRQRGEITMAVQSLGAAAQNALLAAYDLGLDTGWMCAPLFCPEKVVEALGLNP
                     NLVPHALLTLGYAEGDPPKRRARRPLEELIVYRD"
     gene            1393..1722
                     /locus_tag="AC16CM_07765"
     CDS             1393..1722
                     /locus_tag="AC16CM_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139765.1"
                     /translation="MSQASAGGQSRADVERKLIQKSLQDEDFRQKLLAEPKGTLEQEL
                     GTQLPEGVEVRVMEESADTIYLVLPGNASPPEGQSSEISDQELEAVAGGDGGTWGDAC
                     NYTVGHI"
     gene            complement(2307..2735)
                     /locus_tag="AC16CM_07770"
     CDS             complement(2307..2735)
                     /locus_tag="AC16CM_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3139766.1"
                     /translation="MASGEGGGVFNTGTLYQCGATLSGNQAPPGTEDDISGTPAQQCP
                     TQEQRCTISGTRGSDTLLGTARDDVICGRGGTDTLMGRGGDDVLRGGGGPDALVGDSG
                     SDGLFGGAGSDALLASDGVRRNDAMTGGTGRDRCVGDQGT"
     gene            3052..3882
                     /locus_tag="AC16CM_07775"
     CDS             3052..3882
                     /locus_tag="AC16CM_07775"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3139767.1"
                     /translation="MDEPDVASLRSRREDVDRGLWWGKEGKFPASLPARRFHIIGVGL
                     PKTGTTSLASLFQPFRWGMEFLFAESVARLLAWKGGRLGHERIRRWLALRAEAGALEM
                     DSASFNHNFLEILVEMHPAAHFVYTHRDPVSWTDSFLNMTLRQGKQHGVREWPLWQVG
                     LGSLMAPSFDPELFVSPERLRLSLRPLAEELLGYYARETARIEAALPPERSLVIPTED
                     LSTSFDTLAEFVGVPKAALNEAGSHAGRSQGNIPLVAALPDLPHLLERNGLGAAYPLP
                     "
     gene            4093..4428
                     /locus_tag="AC16CM_07780"
     CDS             4093..4428
                     /locus_tag="AC16CM_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139768.1"
                     /translation="MSEAPASDARRGEAERNLVQRSLQDEAFRQKLLDDPRGAVEQEL
                     ATQLPESVEVRVVEESTDTIYLVLPSAAPIGEVGEITDEELELVAGGVPGHNVWSGTM
                     DSCMMGNPC"
     gene            complement(4820..>4934)
                     /locus_tag="AC16CM_07785"
     CDS             complement(4820..>4934)
                     /locus_tag="AC16CM_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR3139769.1"
                     /translation="ATVKSHLIHIFDKLGVADRTAAVTVALERGILRLPSR"
BASE COUNT          902 a         1574 c         1650 g          808 t
ORIGIN      
        1 ttcgcctcgg aggtaatgcc gagggtagac gaagcggtat cggcttgaag gcaacccccg
       61 gcgcactgag cggggagcag atcgctttcc tggtccggca gagggtggcc cgcctcgcga
      121 ccgccgacgc tgcgggacgt ccccacgtcg tacccgtgtg cttcgccttc gaccaatctt
      181 ccgtatacat agccctcgat gagaagccga agaaggtgcc ggcctccgac ctcaaacggg
      241 tcaggaacat cctggcgaac cctgaggtcg ccctgaccgc cgaccgttac gccgaagact
      301 ggagcctgct cgccttcttg atggtacagg gcgtcgccga aattctggag cccgacaccg
      361 atgagcacgc cgctgccgtg cggctcctga ggggcaagta ccaccagtac gagacgatgc
      421 ggatacagga gaaccccgtc atagccattc gtcctgagag ggccgtcgcg tggggcgcac
      481 tcgatgaacc aggatcacac gggccagggg tgctcgacac catccaaggc cgcaggtccg
      541 tccggaagta tttgcccaaa gaggtgacgg atggggtcgt ggagcaggtg ctcgaggccg
      601 cccgctgggc cccttctccc cacggaaggc agccctggcg cttcgccgtg atcagacggg
      661 acgagaccag ggagcggctg gccgacgcga tgggcgaaga gtggcgcgcc aacctggaga
      721 tggacggcca gagccaggag atcgtcgagc ggcgcctcga aggctcgcgc aggcgtctgc
      781 tggacgcgcc ggttctcgtg ctgctgtgcc tctacctcga agacctcgac gaataccccg
      841 acgccgggcg tcagcgtggt gagatcacga tggccgtcca gtcgctcggt gccgccgccc
      901 agaacgcgtt gctcgcggcc tacgacctcg gtctcgacac gggctggatg tgcgccccgc
      961 tcttctgccc ggagaaggtg gtcgaggcgc ttgggctcaa cccgaacctc gtcccacacg
     1021 cgctgcttac gctcgggtac gcggagggtg acccaccgaa aaggagagcg cgcagaccgc
     1081 tcgaggagct tatcgtctac agggattgat acggctccgc gagtgcctct cgatggtgca
     1141 aagtcccgag agcgcgtggg agaccagccc atcgagcggg agaacaggac aaccgcctcg
     1201 cgctctcagt ccaggtcggt gagaagccct cctgcccaag acgaagttgg tctggcaacg
     1261 ggccaggagc agcccttgcg cggagcacac gaccgtgcta ggtgtgatac cgactcgcga
     1321 tgggagcagg tctcaggaca acgacggacc gctcctgggt ggccggggac acacagcgaa
     1381 gggagagggg ccatgagcca agcatcagcc ggtggccaaa gccgcgccga cgtggagcgt
     1441 aagctgatcc aaaaaagcct acaggacgag gacttccgcc aaaagctgct cgcggagccc
     1501 aaagggaccc tggagcagga gctggggacg caactgcccg agggcgtgga ggtaagggtg
     1561 atggaggaga gcgcggacac catctacctg gtgcttcccg gcaacgcctc gccgcccgaa
     1621 ggccagagca gcgagatctc cgaccaggag ctggaggccg tggccggcgg cgacggtggc
     1681 acctggggtg acgcctgcaa ttacaccgtg ggacacattt aacgagaacc tgaagtcgtt
     1741 tgtccatatg gccgccgcct ctctgctcgg ttgcacctgg tgtctcggct tcgactcgtt
     1801 ccaggcgcaa agcgagagcg tcgtctaggc gaacttccat gagcggcggg gttgtcaacg
     1861 gttatggtgg aagcccaggc ggggctcgaa gcgtaggcga gcacgagcgc ccacaccaac
     1921 aaggccgccc ccacgaaggc cgccatcttc gacgggcgaa tcgcctcacg ggtcatcgtc
     1981 ttccgggcac ccctttccta gccagaacca ccatcgccgt ggcgttcttc gcggcgagct
     2041 ccgtcgcctt ccccgtcgct tgaagccgtc cggctacgct cgcatctctt tcatggacca
     2101 acggtaggaa ttcctcaccc cacctgcacc cataaaatga tgagtatctc cggaagatct
     2161 ggagcttttt cgggaggagc ttctcggagc accgtcaacg cacagttcgg agggcaaacg
     2221 ttccccactc cgctggatag cggagcgggg gaccgaaagg agcttccacc ccaggaagat
     2281 gcgctcctac tatcggcagc ttctctttac gtcccctggt cacctacgca ccggtccctt
     2341 cccgtgccac cagtcatcgc gtcgtttctc cttaccccgt ccgaggcgag cagggcgtcg
     2401 gagccagccc cgccgaagag cccgtccgaa ccggagtcgc ccacaagggc gtcgggaccc
     2461 ccgccgccgc gcagcacatc gtcgccaccg cgccccatca gcgtgtcggt cccgccccgt
     2521 ccgcagatca cgtcgtcgcg ggcggttccc aggagcgtgt cggacccgcg ggtgccggag
     2581 atggtgcagc gctgctcctg cgtcgggcac tgctgggccg gggtgcccga gatgtcgtcc
     2641 tcggtgccag gaggcgcctg gttgccgctg agcgtggcgc cgcactggta aagggtgccc
     2701 gtgttgaaga cgcctccacc ctctccggag gccacgttcg agctttggtg aacaaggcac
     2761 gaaaaggtct gggccgttgg gcccgaccat cctcgtgcta ctcccagggg acactctggc
     2821 gcaggcaccc ttgcgcggag cacacgacca tgcaagctgt gtgatactgc cccgcggtgg
     2881 gagcgggcct cagagcgccg agacgctgcc cccaggcggt cgaagacaat gcgaagggag
     2941 agaagattgt gaccgtagcg agcggtggcg ccaggcgcgg cttgctccgc ctccctggcc
     3001 aatcgaggtc gaccgcagcc ttccgtctcg gtggtacctg ggagagatcc gatggatgaa
     3061 cccgacgtcg cctcgctgcg gtcgcggcgc gaggacgtcg accgcggcct ctggtggggt
     3121 aaagagggaa agttccccgc ctcactaccc gccaggcgct ttcacatcat cggggtcggt
     3181 ctgcccaaga cggggacgac ctcgctggct tccctgtttc agcccttccg ttggggcatg
     3241 gagttcctct tcgccgaatc cgtcgcgcgc ctcctcgcct ggaagggtgg caggctcggc
     3301 cacgagcgca ttcgccgctg gctagccctg agggccgagg ctggagcgct ggagatggac
     3361 tcggctagct tcaatcacaa ttttctggag atactggtcg agatgcaccc cgcagcccat
     3421 ttcgtctaca ctcaccgaga tcccgtctcc tggaccgatt cttttctgaa catgacgttg
     3481 cggcaaggca agcagcacgg ggtcagggaa tggcccctgt ggcaggttgg gctcggcagt
     3541 ctcatggctc ccagcttcga tcccgaactc ttcgtctccc ccgaacgtct ccgcctctcg
     3601 ctccgcccgc tcgcggagga actgctgggt tactatgcgc gcgagacggc ccgtatcgag
     3661 gccgcgcttc cgcccgaacg gtcgctggtc atccccaccg aagacctttc cacgagcttc
     3721 gacaccctcg cagagttcgt cggggtgccg aaggcggccc tgaacgaagc gggatcacac
     3781 gcgggccgca gccagggcaa catccccctc gtggccgcgc tgcctgacct gcctcatctc
     3841 ctggagcgga acgggttggg tgctgcgtac ccgttgccct gaagcgggta gggtaggcgc
     3901 cgcccggaaa agcgagccgg tatggccgag gcttccgcga atcaagaggg tagcatgcct
     3961 ctccccgagt agctggccgt accgagccag gagcagccct tgcgcggaga agagcactat
     4021 gctagatatg gtccaatctg cgatgggagc atgccccaga gcgccagacg agcacagcga
     4081 agggagagga ccttgagcga agcaccagca agtgacgcca ggcgtggcga ggcggagcgc
     4141 aatcttgtgc agaggagcct ccaggacgag gccttcaggc agaaactgct tgatgatccc
     4201 aggggtgccg tggagcagga gttggcgacg caactgcccg agagcgtgga ggtaagggtg
     4261 gtagaggaga gcacggacac catatacttg gtgcttccca gtgctgcgcc gatcggtgaa
     4321 gtcggtgaga tcactgacga ggagcttgag ctggttgccg gcggggtacc gggacacaac
     4381 gtatggagtg ggacaatgga ctcgtgcatg atgggtaacc cttgctaatg atgtccatga
     4441 ccgtcgccca gcatggagtt ccgctttgcg acggtgccca aaaccccggg ctcagctacc
     4501 agcgacttga gcccggctta cgccagccct ttgggcacag gctgcaggag ggggtctacg
     4561 tggtcgtggg cggcagtacc agggtaaagc tggacgctgg ggtatcatag ctcgagcgct
     4621 gggttgctgt ccgcgacggc acacgcacag caacccagtt cgaggccggt gccgatgggg
     4681 ccgggatcct cgcgttcgga gcaccgaaca cggggacttc gtggctgccg ataccgggcc
     4741 gccccggagt ggtggacggg ttgacctggc aagggcgcaa cggggtcatg cgcaaaccac
     4801 cccggcgagt tacgccgggt taacggcttg gcaggcggag gatgcctctc tccagggcga
     4861 cggtgacggc ggccgttcgg tcggcgacgc ccagcttgtc gaagatgtgg atgaggtggg
     4921 atttcaccgt ggcc
//
LOCUS       DBNGVD010000289         2133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_612495, whole genome shotgun sequence.
ACCESSION   DBNGVD010000289 DBNGVD010000000
VERSION     DBNGVD010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_612495"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..151
                     /locus_tag="AC16CM_07790"
     CDS             8..151
                     /locus_tag="AC16CM_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3139770.1"
                     /translation="MPLVSIVIGAIFLGERPTTSLLVGTFLIVTGIYTVNRPSTGKGK
                     LAN"
     gene            complement(457..1341)
                     /locus_tag="AC16CM_07795"
     CDS             complement(457..1341)
                     /locus_tag="AC16CM_07795"
                     /inference="COORDINATES: protein motif:HMM:NF021287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR3139771.1"
                     /translation="MDIRHIDIDFEPKLERPILIAGFTGWNDAADAASVAVETLASSW
                     DARRFGGFDGEEFFDFQTTRPQVKLVDGVTREIEWPENTLSATQASLEATGGRGVVLL
                     SGPEPNFRWRTFSQTVVDLARALDVELVVTMGALLADVPHSRPVSVAANSQDPTLVES
                     LDLSASRYEGPTGITGVLHRVCADGGLPSVSFWASVPHYLPAVPSAPATIALLEKLSG
                     LLGMKVDTTELESTAETYQDQVSVAVSQDSDLSSYVKMLEERYDSQLDQGSRNLPSGD
                     DLAQELEGFLRDHRREEE"
     gene            complement(1386..1958)
                     /gene="aat"
                     /locus_tag="AC16CM_07800"
     CDS             complement(1386..1958)
                     /gene="aat"
                     /locus_tag="AC16CM_07800"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR3139772.1"
                     /translation="MRLTPQILEACYRAGAFPMADPYGHIGFYRSDPRSVLELDELHV
                     SRSLARVLRKGTHEVRVDEDFEGVIRACADREETWINAEIIEAYVRLHAAGKAHSVEA
                     YSEGNLVGGLYGVTLGGVFMGESMFTRMRDASKVCFVHLVCRLRERDFRLLDCQIQNP
                     HLASLGAREIPEEEYLRRLQHALGLERSFL"
BASE COUNT          384 a          686 c          684 g          379 t
ORIGIN      
        1 tttcttcgtg cccctcgtca gcatagttat cggggcgatc ttcctcggcg aacggccaac
       61 gacctcgctc ctggtcggga ccttcctgat cgtcactggc atctacaccg taaatcgccc
      121 gtcaaccgga aaaggcaaac tcgccaacta ggcccgaact tcccctctgt ttggcgacgc
      181 ctcgaaggtg gtcccggtct tgccgagctc ctccgcgacc gagcatcgcg acgacggccg
      241 gccgggattt cagaggctgg gcacgggtag atgtccccgg cggttacaga aacctgcaac
      301 gcttccacgg cgagttcagg tcatcgggat acacgtcacg gtgcgacttc gtgccggtgc
      361 cggtagcaac gcgattacag gtttcgagca aaccgccgtg cccaagcgcg attatgtcac
      421 gcgagtgccc aaagcttgct gcctgaaacc tgtagtctat tcctcctcgc ggcggtggtc
      481 gcggaggaag ccttccagct cctgggccag atcgtcacca gatggcaggt tgcgcgagcc
      541 ctggtcaagc tgcgagtcgt agcgttcctc gagcatcttt acgtacgagg acagatccga
      601 gtcctgagag acggctacgg agacctgatc ctggtaggtc tcggccgtgc tttcgagctc
      661 ggtggtgtct accttcatac cgagcaaccc ggagagcttc tcgaggaggg cgatggttgc
      721 cggggcggac gggacggcgg gcaggtagtg tggaaccgag gcccagaagc taacggaggg
      781 aagccccccg tcggcgcaga cccggtgcag cacgcccgtg atacccgtcg ggccctcgta
      841 acgggaggcg gagaggtcca atgactcaac aagggttggg tcctgggagt tggcggcgac
      901 cgagacaggg cgcgagtgcg gcacgtccgc cagcagggca cccatcgtca cgaccagttc
      961 tacgtctaga gccctggcca gatcgacgac cgtctgggag aacgtgcgcc accggaaatt
     1021 cggttccggc cccgacagca agacgacccc gcggcccccg gtggcctcca ggctagcctg
     1081 cgtcgcggag agcgtgttct cgggccattc gatctcgcgc gtcaccccgt cgacgagctt
     1141 gacctggggg cgggtggtct ggaagtcgaa gaactcctcg ccgtcgaagc cgccgaaccg
     1201 ccgtgcgtcc cacgagcttg ccagcgtctc gacggcgacg ctcgcggcgt ccgcggcgtc
     1261 gttccaacct gtaaaaccgg cgattaggat cggacgctcg agcttcggct cgaagtcgat
     1321 gtcaatgtgt cgtatatcca tggggcaagc ttaccacgcg ccggaagtgg tcagccgggt
     1381 caggattaca agaaagagcg ctccaggcct agggcgtgtt gcagccgcct gaggtactcc
     1441 tcctccggta tctcgcgcgc cccaaggctt gccaggtggg ggttctggat ctggcagtcg
     1501 agcaacctga aatctctctc ccgcaggcgg cagacgaggt gaacgaagca gaccttggag
     1561 gcatctctca tgcgcgtgaa catcgactcc cccatgaaca caccccccag ggtgacgccg
     1621 tagagccccc cgacgaggtt gccctcgctg taggcctcga cgctgtgggc ttttcccgcc
     1681 gcgtgcagcc ggacgtaggc ctcgattatc tccgcgttga tccaggtctc ctccctgtcg
     1741 gcgcacgcgc ggatgacgcc ctcgaagtcc tcatcaacgc gtacctcgtg ggtccctttg
     1801 cggagcacgc gggcgagcga ccgggagacg tgcaactcgt cgagctccag cacgctccgc
     1861 gggtcggagc gataaaaccc gatgtggccg taagggtcgg ccattggaaa cgcgccggcc
     1921 cggtagcagg cctcgagtat ctgcggggtc agcctcaagc ggcgggaagt cctaaccggg
     1981 gtttcgggcg aagatgaggt gggttcccct tcctggcacc ggtacgatct ctacgccctc
     2041 gaagccggca tccgtgagcc aaccctgata atctccacgg gtgtaggttc ccccgccggc
     2101 cgtcaggacc agcatgttta cggcgaagag ggc
//
LOCUS       DBNGVD010000290         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1523828, whole genome shotgun sequence.
ACCESSION   DBNGVD010000290 DBNGVD010000000
VERSION     DBNGVD010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1523828"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="AC16CM_07805"
     CDS             complement(<1..673)
                     /locus_tag="AC16CM_07805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016413 - O-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase family protein"
                     /protein_id="HIR3139773.1"
                     /translation="MTLDSGAGASHAPALQGKTNYPIGPFEPELGGHIPEATLAYETW
                     GQLNEAADNAVLLVHALTGDSHATGGPNETHRRGGWWDPMVGPGRPIDTNEYFVVCSN
                     VLGGCSGSTGPASVDPESGRPYGMRFPVVTIRDMVRAQKRLLDALGIRRLALVAGGSI
                     GGQQALEWAVEFPDFVEKAVPVAATGALGPQGVGMSEMGRRAIMADPDWQEGDYYGTG
                     RSPNEG"
     regulatory      complement(770..886)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1003..1977)
                     /locus_tag="AC16CM_07810"
     CDS             complement(1003..1977)
                     /locus_tag="AC16CM_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139774.1"
                     /translation="MSNQEGDSVHTLRAGWPRRIFGLRVVLLLSAVAAGAGLAIALGL
                     SGARAESYSAYDDPQKPALSFLLSDDQNAEEFQKEFGLGDEQMQKVLALANRERTTLD
                     KAYDDSDRIIEANRGASDSKIRGKISASGFDEKLKKAIAKTKSDVGKLLPEGRTADLG
                     PWVDEQWLAEGAEYEAVSEASYSATSAGYKCRVWMSYYRGLTRYEVALPHKKVKFSGG
                     KKVRITDIRKGTSARAPVKDSGPWNIRDNYWRGSKYRSMWKDLPRCVPEAQAAFFNNY
                     NRGKDQFGREVGNPAGIDITPGVARRMDIWPKMQRRGLIKVRVQYLWG"
     gene            complement(2043..>2212)
                     /locus_tag="AC16CM_07815"
     CDS             complement(2043..>2212)
                     /locus_tag="AC16CM_07815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835370.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3139775.1"
                     /translation="KRGFVVAPWDGSRETEQKIKEDTKATIRLLPFEREEGEDLVSGK
                     PGLTAIFARAY"
BASE COUNT          344 a          758 c          661 g          449 t
ORIGIN      
        1 ggccctcatt ggggctgcgg cccgttccgt agtagtcgcc ttcctgccaa tccggatcgg
       61 ccatgatggc gcggcggccc atctcgctca tccccacccc ctgcggcccg agggcaccgg
      121 tggctgcgac aggcacggcc ttctcgacga aatcagggaa ctcgacggcc cactccagcg
      181 cctgctggcc gcctatggag ccgcccgcca cgagggcgag cctgcggatt ccaagggcgt
      241 cgagcagacg cttctgggcc ctgaccatgt ccctgatcgt aaccacgggg aaacgcatcc
      301 cgtaggggcg cccactttca ggatccaccg aggcgggccc cgtgcttccc gagcaaccgc
      361 cgagcacgtt ggagcagacc acgaagtact cgttggtgtc tatcggacgc cccggcccga
      421 ccatagggtc ccaccagccg ccgcgcctgt gtgtctcgtt gggccccccg gtggcgtgtg
      481 agtcacccgt cagggcgtgg accaggagaa ccgcattatc tgcggcctcg ttcagctggc
      541 cccaggtttc gtaggccagt gtggcttcgg gaatgtgccc gccgagctcc ggctcgaagg
      601 gacctatggg gtagttggtt ttaccctgga gggccggggc gtggctagcg ccggcccccg
      661 agtctagtgt catattcggc agtaatgtag cctcctctcg gtcgggttac acgccttcgg
      721 cgcaacgcct ctcgcgcaac ccgctcgtag aagaggctgc ccgcctctcc ccacatcttc
      781 caggacggta atgatcgtcc tgctggactt ggcaccttgc gttccgtgct accgccggtt
      841 gccgcggttt ctcggggcca gttccctccg ccgctctcga tgcgagttcg cgctaggcgt
      901 ggatgctaac ccacccgagg tcttagcgtc aagggccgaa agggcaccca tcgggacgcc
      961 gggccgaaag gtgcgttcca ggggtcgccg gccgcctgtg cgtcatcccc agagatactg
     1021 gacccggacc ttgatcagcc cgcggcgttg catcttgggc cagatgtcca tccggcgggc
     1081 cacgccgggg gttatatcga tgccagccgg gtttccgacc tcccgcccga actggtcttt
     1141 gcccctgttg taattgttga agaaggcagc ctgcgcctcg ggtacgcagc gtgggaggtc
     1201 tttccacatg ctcctgtact tcgagcccct ccagtagttg tcccggatgt tccagggacc
     1261 ggaatccttc acaggtgccc gagcgctggt gccctttcta atgtcggtga tcctgacctt
     1321 ttttccaccg ctgaacttga ccttcttgtg cggtagggcc acctcgtacc tggtgaggcc
     1381 cctgtaatag gacatccaca ccctgcactt gtaaccggcc gacgtggcgg aatacgaagc
     1441 ctcgctcacg gcctcgtatt ccgcgccctc ggccagccac tgctcatcaa cccaggggcc
     1501 gagatccgcc gtcctgccct ccggcagaag cttcccgacg tcggacttgg tcttggcgat
     1561 ggctttcttc agcttctcat cgaaaccgga ggcagagatc ttgcctctta tcttcgagtc
     1621 cgaagcgcct ctgttcgcct cgatgatcct gtcgctgtcg tcgtacgcct tgtctagcgt
     1681 ggtcctctct ctgttggcca gggccagaac cttttgcatc tgctcgtcgc ccaggccgaa
     1741 ctccttctgg aactcctcgg cgttctgatc gtctgagagc aagaaggaga gcgccggttt
     1801 ctggggatcg tcgtatgcgg agtagctctc cgcccgggcc cccgacagac cgagcgcgat
     1861 cgcgaggcca gccccagccg caacggcaga cagcaacaac acgacccgta ggccaaagat
     1921 ccggcgcggc cagcccgcac gcaatgtatg cacggagtcc ccctcctgat tgctcaacgc
     1981 cacgaatttt acacgttcgt gcgtatgtta cgagctggcc ccgtcggaag gcagtcccct
     2041 cctcagtacg cccgggcgaa gatcgcggtc agacccggtt ttccggagac gaggtcctcg
     2101 ccctcctcgc gctcgaaggg gaggagccgg atcgtggcct tggtgtcctc tttgatcttc
     2161 tgctccgtct ccctgctacc gtcccagggg gcgacgacga agccacgttt tt
//
LOCUS       DBNGVD010000291         3634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4438541, whole genome shotgun sequence.
ACCESSION   DBNGVD010000291 DBNGVD010000000
VERSION     DBNGVD010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4438541"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1478)
                     /locus_tag="AC16CM_07820"
     CDS             complement(282..1478)
                     /locus_tag="AC16CM_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR3139776.1"
                     /translation="MGAREGDNRARAGGVGPRGWLRERVEGRTQVRQVELFFDLVYAL
                     AVTQLTYHLLARMSFRGALETLLLLLAVWTTWVYTGWITNYFDPDTRPMRLMLLGAAF
                     ASLVMSASIPAAFDDRGLLFATAIATIQVGRTWFVLAGLGRGHHMSGNFQRAMIWWSA
                     TGVLWMAGGLVAGDLRIVVWMAAVVAQYVGVWIGFPVPGLGRSRTTDYTIVGGHLAER
                     CQLFILLALGESILVIGADFGELAPWGPTALAFAVAFAGSVALWWIYFDRGAEAGRRA
                     MSTAADPGRLGISAYNYCHVPMVAGIIVAAAADGLTMAHPTGRVTVGTTALILGGFAL
                     YLLGNALFEWVLGGHLPRSRLIAVCALAALLPLSLATSALVLLSAATLIMVCVAIRDE
                     RVPEGA"
     gene            complement(1547..>3634)
                     /locus_tag="AC16CM_07825"
     CDS             complement(1547..>3634)
                     /locus_tag="AC16CM_07825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3139777.1"
                     /translation="LLILAGAGSGKTRVLTHRVAHLIDQGLAAPEEVLAITFTNKAAR
                     EMKERVALLVGPDSRRMWVSTFHAFCARILRVHAEKLGYKREFTIYDGADQVRLVKRC
                     IVELGKDPKRFSPRSFQAQISSAKNVLMAPDDFLRNTEGYMAENVAEVYDLYQKRLYE
                     NNAMDFDDLIMQTVALLELFPEVRERYQTRFKYIHVDEYQDTNHAQYRLVNILAAAHR
                     NLCVVGDDDQSVYSWRGADIRNILDFERDYPEAKVVKLEQNYRSTQTILDAANAVVAN
                     NASRKAKELWTAGDEGERIRVFAASDEYAEARFVVSEIERLMDDGTSARDISVFYRTN
                     AQSRALEDVLVREGIPYQVVGGVRFYERAEIRDAMAYLAVIANPDDSGSLERIINVPK
                     RGIGNTSVAKLQDHANKSGITLYEALTEADAAGLTGAAKKACKAVRDLFEGLRVAALE
                     VPPTDLIGAVLDESGYRKELEAEDTIEAESRLENLEELINAAREYERVEPEPTLAGFL
                     QEQALYSDQDSMTSEGGSVTLMTLHNAKGLEFSHVFVVGMEEGTFPHARSLDEQNLEE
                     ERRLAYVGITRARETLTLTHARLRSSWGEREYRMPSRFLSEIPDEYKSGTVPSTLTGA
                     TGRGGWGVARSGGRGPQAPASGGTQYRAGERVRHAKFGVGEVVEADASRIVIRFGTEE
                     RTFVPEIAPLSKI"
BASE COUNT          623 a         1224 c         1156 g          631 t
ORIGIN      
        1 gcctcgtagt agttgtccgg gtcgacgagg ttcttgtact cggcgtatcc gatggccgag
       61 gcgatcgtgt ccgtatccgg gttcttgtgg cccgttacgt aaacgtgcgg cataccactc
      121 cttttccctc atccgaacgc ggttccagtg gccgggcttg ggcctagatc gtcgcccttc
      181 cgcagtaacc gaatcgctgt ttctgctccg caaagcgtat cagataccgt gccagacgcg
      241 gcgcgcgacc tttcccgaca gagcccgtgc gatccccggc gtcatgctcc ttcggggaca
      301 cgctcgtccc ggatcgccac gcagaccata atcagggtcg cggcggagag gagcaccagc
      361 gccgaagtgg cgagggacag aggcaggagc gcggcgagcg cgcagacggc gatcagcctc
      421 gagcgcggca ggtggccacc gagcacccac tcgaacagcg cgttgccgag cagatagagt
      481 gcgaaaccgc ccaggatcag ggccgtcgtc ccaaccgtta ccctgcctgt cgggtgtgcc
      541 atggtcagac cgtctgcggc ggcggccacg atgatgccag ccaccatcgg gacgtggcag
      601 taattgtagg cggagatccc caaccgcccc gggtcagcgg cggtggacat cgcacgccgg
      661 ccggcttcgg caccgcggtc gaagtagatc caccacagcg cgacgctgcc cgcgaaggcc
      721 acggcgaaag caagggcggt cggaccccag ggggcgagct cgccgaaatc ggcgccgatc
      781 accaggatgg actcgccaag cgccaggaga atgaacaact ggcagcgctc ggccaggtgc
      841 ccgccgacga tggtgtagtc cgtcgtgcgc gaacggccca ggccgggcac gggaaagccg
      901 atccagacgc caacgtactg cgccaccacg gccgccatcc acaccacgat tcgcagatcg
      961 ccggctacca gccctccggc catccacagc acccccgtcg ccgaccacca gatcatcgct
     1021 ctctggaaat ttccgctcat gtggtggccg cgcccgaggc cggcaagcac gaaccaggta
     1081 cggcccacct ggatagtggc gatggccgtg gcgaagagta ggcccctgtc gtcgaaggcg
     1141 gcgggtatcg aggcggacat gaccaggctc gcgaacgcgg ccccgagcag catcagacgc
     1201 atgggtcgtg tgtccgggtc gaagtagttg gtgatccatc cggtatagac ccaggtggtc
     1261 cacacggcca ggagcagcaa cagcgtctcg agggcgccgc gaaacgacat gcgggccaac
     1321 agatggtacg tgagctgcgt taccgcgagg gcgtagacca ggtcgaagaa caactcgacc
     1381 tgcctgacct gggttctgcc ttcgacgcgc tcgcgcagcc agccgcgggg ccccacacca
     1441 ccagctctcg cccggttgtc tccttccctg gcgcccacag ggcacaggat gccacgccca
     1501 gaccacggaa tgaatagcca gccgccggta acgccccggc gggacgctag atcttgctga
     1561 gcggcgctat ctcgggaacg aaggttctct cctcggtccc gaaccggatc acgatcctgc
     1621 tcgcgtcggc ctcgacgacc tcccccaccc cgaacttggc gtggcggacc cgctccccag
     1681 cccggtactg cgtcccgcca gaagcgggag cctggggacc ccggcccccg gagcgcgcca
     1741 cgccccaccc gccgcggccg gtggcgccgg tgagggtgct cggcacggtg cccgacttgt
     1801 actcgtccgg tatctccgag aggaagcgcg agggcatcct gtactcgcgc tcgccccagc
     1861 tggagcgcag cctggcgtgg gttagcgtga gcgtctccct cgcgcgtgtg atgccgacgt
     1921 aggcgagccg ccgctcctcc tcgaggtttt gctcgtccag ggagcgggcg tgcggaaagg
     1981 tcccctcctc catccctacg acgaagacgt gggagaactc caggcccttg gcgttgtgca
     2041 gcgtcatcag cgtcacgcta ccgccctcgg aggtcatgct gtcctgatcc gagtacagcg
     2101 cctgctcctg caggaagccg gcgagcgttg gctcgggctc gacgcgctcg tactccctgg
     2161 ccgcgttgat tagctcctcc aggttctcaa ggcgcgattc ggcctctata gtgtcctcgg
     2221 cttcgagctc tttcctgtac ccggattcgt ccagcacagc cccgatcagg tccgtcggtg
     2281 gcacctcaag ggcggccacc ctcagcccct cgaacaggtc acgcacggct ttgcaggcct
     2341 tcttcgccgc gcccgtcagg cccgccgcgt cggcctccgt cagcgcctca tagagggtga
     2401 ttccgctctt gttggcatgg tcctggagct tggccaccga ggtattgccg ataccccgtt
     2461 tggggacgtt gatgatgcgc tccagcgaac ccgagtcgtc ggggttggcg atcaccgcca
     2521 ggtaggccat ggcgtcccgg atctcggccc gctcgtagaa gcgtaccccg ccgacgacct
     2581 ggtagggaat cccctcccgc accagcacgt cctcaagagc ccggctctgg gcgttggtcc
     2641 tgtagaacac ggagatgtcc cgcgccgacg tcccatcatc cataaggcgc tcgatctccg
     2701 agaccacgaa ccgcgcctcc gcgtactcgt cggaggcggc gaacaccctg atcctctccc
     2761 cctcatcccc agcggtccac aactccttgg ccttgcgcga agcattgttg gccaccaccg
     2821 cgttagccgc atccaggatg gtctgcgtgg aacgataatt ctgttccaac ttcacgacct
     2881 tagcctcggg atagtcccgc tcgaagtcga ggatgttcct gatatccgct ccgcgccagc
     2941 tatagactga ctgatcgtca tcgccaacga cacacaagtt acgatgtgct gcggccagga
     3001 tgttgacgag gcggtactgg gcgtggttgg tgtcctggta ctcgtctacg tggatgtatt
     3061 tgaagcgggt ctggtagcgt tcccggacct cggggaagag ctcgagaagg gccacggttt
     3121 gcatgatcag gtcgtcgaaa tccatggcgt tgttctcgta tagacgcttc tggtagaggt
     3181 cgtagacctc ggccacgttc tcggccatat aaccttccgt gttgcgcaag aagtcgtcgg
     3241 gtgccatgag cacgttcttg gccgagctga tctgggcctg aaacgagcgg gggctgaagc
     3301 gcttggggtc cttgccgagc tccacgatgc accgtttgac gaggcggacc tggtcggcgc
     3361 cgtcgtagat ggtgaactcc ctcttgtagc cgagcttctc ggcgtggacg cgcaggatgc
     3421 gggcgcagaa ggcgtggaag gtggagaccc acatcctcct ggagtcgggg ccgactagca
     3481 gcgccaccct ctccttcatc tccctggcgg ccttgttggt gaaggtgatc gcaagcacct
     3541 cctcgggggc ggccagcccc tggtctatga ggtgggcgac gcgatgagtg agcacgcgcg
     3601 tcttcccgct gccagccccg gcaaggatga ggag
//
LOCUS       DBNGVD010000292         2143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2070684, whole genome shotgun sequence.
ACCESSION   DBNGVD010000292 DBNGVD010000000
VERSION     DBNGVD010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2070684"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="AC16CM_07830"
     CDS             complement(<1..427)
                     /locus_tag="AC16CM_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3139778.1"
                     /translation="MQTEGVDARRAVEELKELADLTGGPEGARRVAWTDEWQKAREWF
                     RGKLEEIEGITEIETDEAGNVWATAPGDSERAVLLGGHIDSVPNGGWLDGALNVVGAL
                     EVMRALAPQERPVTLRLVDWADEEGARFGRSLFGSGAAAG"
     gene            complement(427..1734)
                     /locus_tag="AC16CM_07835"
     CDS             complement(427..1734)
                     /locus_tag="AC16CM_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3139779.1"
                     /translation="MTDPGELFDRHKEALPSWMPLYYEHPLELQRGEGFKVWDSEGNE
                     YLDFFGGIVTTISGHAVPEITDAIKSQVDKILHTSTLYLIENQIKLAEKLIELAPISG
                     EKKAFFVGSGSEANEAALLFATRYRNSGEIIALRSSYHGSTFMTMGITGQSVWSPTSR
                     SALDVAYARNPYRLRCPLCRGEGRCNLLCADDVRAVVEEETTGNVAAFIAEPIQGVGG
                     FVDAPPGYFERVKEILDETGILLISDEVQTGFGRTGDHFWGIDAFDVEPDMIVMAKGL
                     GNGLAIGAVVGRAEVIESVSPNLHLSTFGGNPISTAGALANINYVLDNDLQSNAHAVG
                     THLKGRLEKMAENHDVVAEIRGRGLMLALELVGDGLEPDPAAAARFLEACRRRGLLVG
                     KGGLKANSIRISPPLIVTREAADEAADIMDEALAEVGATEKVG"
     gene            complement(1731..>2143)
                     /locus_tag="AC16CM_07840"
     CDS             complement(1731..>2143)
                     /locus_tag="AC16CM_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3139780.1"
                     /translation="FTLIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQARI
                     YGAYPRKGTLAPGADADMVIWDPELSITATLENRHGNVDYTPYEGREFHGGPEAVYVR
                     GELVYRDGEVVGERGSGRFVERRFKATEGLEVRV"
BASE COUNT          319 a          738 c          695 g          391 t
ORIGIN      
        1 cgccggcggc ggcgccagat ccgaagaggc tgcgtccgaa tcgggcgccc tcctcgtcgg
       61 cccagtcaac gagacgcagg gtgaccggcc gctcttgcgg ggcgagggca cgcatgacct
      121 cgagcgcgcc caccacgttc agggcgccgt cgagccagcc accgttcggg acggagtcta
      181 tgtggccgcc aaggagcacg gcccgctcgg agtcgccggg ggccgtggcc cagacgttgc
      241 cggcctcgtc cgtctctatc tcggttatgc cttctatctc ctcaagcttg cccctgaacc
      301 actcgcgggc cttctgccac tcgtctgtcc aggcgacgcg cctggcgcct tctgggccgc
      361 cggtgaggtc ggccagctcc ttcagttcct ctacggcccg gcgggcgtcc accccctccg
      421 tctgcactag cctaccttct ccgtggcgcc gacctcggcg agagcctcat ccatgatgtc
      481 tgcggcctcg tccgccgcct cgcgggtaac gatgagcggc ggggagatgc ggatgctgtt
      541 cgccttcagg ccgcccttgc cgacgagcag gccgcgcctc cggcacgcct ccaggaagcg
      601 cgcggcggcg gccgggtccg gctcgaggcc gtcacccacc agctcgaggg ccagcatcag
      661 gccacggccg cggatctcgg cgaccacgtc gtggttctct gccatcttct cgagacgtcc
      721 cttgaggtgg gtgcctaccg cgtgggcgtt ggactggagg tcgttgtcaa gcacgtagtt
      781 gatgttggcc agggcccctg cggtcgagat cgggttcccg ccgaaggtcg agaggtggag
      841 gttgggggag acggactcga tcacctcggc cctgccgacg acggcgccga tggccaggcc
      901 gtttccgagg cccttggcca tcacgatcat gtccggctcc acatcgaagg cgtcgatgcc
      961 ccagaagtga tccccggtcc ggccgaaacc cgtctgcacc tcatccgaga tcaggagtat
     1021 ccccgtctcg tccaggatct cttttacccg ctcgaagtat ccgggtggcg cgtcgacgaa
     1081 ccccccgacg ccctggatgg gctcggcgat gaacgccgcg acgttgcccg tggtctcctc
     1141 ctcaacgacg gcccgcacgt cgtcggcaca caggaggttg cagcgtccct caccccggca
     1201 cagcgggcag cggagcctgt acgggttgcg ggcgtaggcc acgtcgaggg ccgagcgtga
     1261 ggtcgggctc cagacgctct gacccgtaat tcccatggtc atgaacgtcg agccgtggta
     1321 ggaggaacgc agcgcgatga tctcgccgga gttacggtag cgcgtcgcga agaggagggc
     1381 ggcctcgttc gcctccgagc cgctgccaac gaagaaggcc ttcttctcgc ccgagatcgg
     1441 ggcaagctcg atcagcttct cggccagctt gatctggttc tcgatcaggt acagggtcga
     1501 ggtgtgcagg atcttgtcca cctggctctt tatggcgtcg gtgatctccg gcaccgcgtg
     1561 gccgcttatg gtggtaacga tcccgccgaa gaagtccagg tactcgtttc cttcggagtc
     1621 ccacaccttg aaaccctcgc cgcgctggag ctcgagcggg tgctcgtagt agagcggcat
     1681 ccacgagggc agcgcctcct tgtgccgatc gaatagctca ccggggtctg tcatacccgt
     1741 acctccagtc cttccgtggc cttgaaacgc cgctcgacga acctgccaga gccccgctcc
     1801 ccgaccacct caccatctct gtagaccagc tctccccgca cgtagaccgc ctcgggccca
     1861 ccgtgaaact ccctgccctc gtagggcgtg tagtccacgt tgccgtgacg gttctctaac
     1921 gtcgccgtga tggacagttc cggatcccat atcaccatgt ccgcgtcggc ccccggagca
     1981 agcgtccctt tccgggggta ggcgccgtag atgcgcgcct ggttggtgga gaggacggcg
     2041 acgaactggt tttcggagag cttgccctcg cgtacgccga gcgtccagag cgtcatggcg
     2101 cgttcttcgg cgccgggtag gccgttgggg atgagggtga agt
//
LOCUS       DBNGVD010000293         3435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3164951, whole genome shotgun sequence.
ACCESSION   DBNGVD010000293 DBNGVD010000000
VERSION     DBNGVD010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3164951"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="AC16CM_07845"
     CDS             complement(<1..664)
                     /locus_tag="AC16CM_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3139781.1"
                     /translation="MSSRVTGADLRERLAVVDENIRGALERGGRSGESVRVLVATKYY
                     APEQLEELARAGVELVGENRAEDLVQKQEEFGDRFEWHFIGHVQRRKARVLVPRVTLV
                     HSVDSVRLVEEISKRAPEGGSDVLLQVNVADEESKYGVSEDEVEGLLEAAADTGGSVR
                     VRGFMTIAPLVEDPEEVRQVFAKLRAIRDRLRESWSPHFDLSELSMGMSGDYEVAVEE
                     GAT"
     gene            complement(661..1422)
                     /locus_tag="AC16CM_07850"
     CDS             complement(661..1422)
                     /locus_tag="AC16CM_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR3139782.1"
                     /translation="MSKTERGTLNGAPAAVHTADGGAVYVAPDPQPEEASLFFFTRLG
                     GVSMPPYDSLNVSRKVGDTPGAVGENLSIIRAAMKGRPAAWVTQVAGDTVLRVSGAGL
                     AGEADALITAEPDLCLSVAVADCVPVALVGEQEVAMVHSGWRGTLTGVSGKTARELRG
                     SPIRAYVGPSIRRCCYEVSQELADRFATVFGREVVSGRYLSLQRAIRTDLEGAGVEVH
                     DLGLCSGCRPDLFYSHRMQKPLTGRNLASIVREAR"
     gene            complement(1437..2516)
                     /gene="ftsZ"
                     /locus_tag="AC16CM_07855"
     CDS             complement(1437..2516)
                     /gene="ftsZ"
                     /locus_tag="AC16CM_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3139783.1"
                     /translation="MLDAATNYLAVIKVVGIGGGGTNAVNRMINSGLQGVEFIAVNTD
                     AQALQMCDADQKIHIGEKITRGLGAGADPKIGMEAAEENKAEIEEALRGADMVFVTAG
                     KGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRSTYAEEGIKRLKENVDSLI
                     IIPNDRLLQVAEKRTSMMDAFKMADDILRKGVQGITDLITVPGLINLDFADVRTIMNN
                     SGSALMGIGESNSETRGPEAAKMAISSPLLEASIEGATGIILNITGGPDLGLFEVNEA
                     AEIVHNAAHQDANLIFGAVIDENNGDRVSVTVIATGFDQRLANARRSERSTVAEPPTQ
                     QAEEESPVSSETGDVLDIPAFLRRR"
     gene            complement(2560..>3435)
                     /gene="ftsA"
                     /locus_tag="AC16CM_07860"
     CDS             complement(2560..>3435)
                     /gene="ftsA"
                     /locus_tag="AC16CM_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3139784.1"
                     /translation="EARQLDLDDDEEVIHVVPRGYVLDGSEGVKNPVGLAARKVTMRA
                     HVVSGAVSSIQNLLRAVEDCGVTASRIVLEPLASAEACLTDADRESGVVLLDVGGGTT
                     DVAIFYRGALTHTDVIPIGGESFTADLAYGLKIPEYKAEELKVRYGTTLSDTIDSVAA
                     VRLDDRHYNASFISQILEYRAQELYDYVQDSLTGIRISDLISGGVVLTGGGSLLDGMT
                     DLAEERLGLRARTAVPRRVRGEVKPVQKPQYATSVGLLYFSARNDDPRPKGKGVGSGS
                     FGNILEAVRDWFRGK"
BASE COUNT          561 a         1174 c         1037 g          663 t
ORIGIN      
        1 cggtcgctcc ctcctcgacc gcgacctcat agtcgccact catgcccatg gagagttccg
       61 agagatcgaa gtgcgggctc caactttctc gtagcctatc acggatcgcg cgaagtttcg
      121 cgaaaacctg gcgtacttcc tcggggtcct cgacgagggg ggcgatggtc ataaagccgc
      181 gcactcgaac gcttcccccc gtatctgcgg cggcctccag cagcccttct acctcgtcct
      241 cggagacacc gtacttggac tcctcatcag ccacgtttac ctgcaggaga acgtcggacc
      301 cgccctctgg ggcacgcttg gagatctcct ctacgaggcg taccgagtca acggaatgca
      361 cgagcgttac ccgcggtacg aggaccctgg ccttgcgacg ttgcacgtgg ccgataaagt
      421 gccactcgaa acgatctccg aactcctcct gtttttgcac gaggtcctcg gcccggttct
      481 cgccgaccag ctctacaccc gcccgggcga gctcctcgag ttgctccggc gcataatatt
      541 tggtggcgac gagcacccgc acgctctcgc cggaacgccc accccgctcc aaggcccccc
      601 ttatgttctc gtccacgacg gcgagcctct ccctgagatc cgcgcccgtc acacgcgagc
      661 tcaccgggcc tccctcacga tagaggcgag gttccggccc gtcaggggtt tctgcatgcg
      721 gtgggagtag aagagatccg gccggcaccc actgcacagc cccaggtcgt gaacctctac
      781 cccggccccc tcgaggtcgg tccttatggc tctctgcagg gagaggtaac gacccgagac
      841 cacctcccgt ccgaacacgg tggcgaacct gtcggccagt tcctgggata cctcgtagca
      901 acagcgccgg atggagggac cgacgtaggc gcgtatcggg gagccccgca gttcccgcgc
      961 cgttttcccg gagacgccgg tgagcgtccc gcgccagcca gagtggacca ttgccacctc
     1021 ctgctccccg acgagcgcga cgggaacgca gtcagcgacg gccacggaca ggcacaggtc
     1081 gggctctgcg gtgatcaggg cgtcggcctc gccggcgagc ccggcaccag agacgcggag
     1141 cacggtgtcc cccgcaacct gcgtgaccca ggccgctggt cgtcctttca tggccgccct
     1201 gatgatggat aggttctccc cgaccgcccc tggtgtgtca ccgacctttc tggagacgtt
     1261 gagggaatcg tagggtggca tagagacacc accaaggcgg gtgaaaaaga agagggatgc
     1321 ctcctcgggc tgcgggtccg gtgcgacgta gacggcgccc ccatccgcgg tgtggaccgc
     1381 ggcgggggcg ccgttcaggg tgcctcgctc ggttttgctc acgccccggc taatgcttac
     1441 cggcggcgga ggaaagccgg gatatctagg acgtcgcccg tctccgagga gaccggcgat
     1501 tcctcctcgg cctgctgggt cggcggttcg gcgacggtgc tgcgctcgga gcggcgcgcg
     1561 ttggcgaggc gctggtcgaa gccggtcgct atcacggtca cgctcaccct gtcaccgttg
     1621 ttctcgtcga tcacggcacc gaagatcagg ttcgcatcct ggtgggcggc gttgtgcact
     1681 atctccgcag cctcgtttac ctcgaagagc ccgaggtcag gcccccccgt gatgttcagg
     1741 atgatcccgg tcgcgccctc gatgctggcc tccaggagcg ggctcgagat cgccatcttg
     1801 gcggcctcgg gccccctagt ctcgctgttc gactccccga tgcccatgag tgcagacccg
     1861 gagttgttca tgatggtgcg cacgtcggcg aagtccagat tgatcaggcc aggcacggtg
     1921 atcaggtcgg tgataccctg gacgcccttc ctcaagatgt cgtcggccat cttgaaggcg
     1981 tccatcatgc tggtccgctt ctctgcgacc tgcaggagcc tatcgttggg gatgatgatg
     2041 agggaatcaa cgttctcctt gaggcgcttg atgccctcct cggcgtacgt ggagcgcctg
     2101 cgcccctcga aggcgaaggg cctagttacg acccccaccg tcagtgcccc ggaatccctg
     2161 gcgatcttgg cgaccaccgg cgccgcgccc gtccccgtcc cgccgccctt gccggcggtc
     2221 acgaacacca tgtcggcccc gcgcagggct tcctcgatct ccgccttgtt ctcctcggcg
     2281 gcctccatcc cgatcttcgg gtcagcgccg gcgccaaggc cgcgggtgat cttctcccca
     2341 atgtgaatct tctggtcggc atcgcacatc tggagggcct gcgcgtccgt gttcaccgcg
     2401 atgaactcaa ccccctgtaa accggagttg atcatgcggt tcacggcgtt ggttcccccc
     2461 ccgccaattc cgacaacctt tattaccgcc aagtagttag tcgccgcgtc caacatgcct
     2521 ttctaaccct ccatcacgca ttctggggga cccaccggtt tacttgcccc ggaaccagtc
     2581 cctgaccgcc tccaatatgt taccaaaact ccccgaccca acacctttac cctttggtct
     2641 agggtcatcg tttctggccg agaagtataa cagaccgacc gaggttgcat actgaggttt
     2701 ctgtacaggt ttaacctccc ctctaaccct tcgtgggacg gcggtgcggg cgcgcagccc
     2761 caatctttct tcggcgaggt cggtcatacc atcgaggagg gaaccaccgc cggtaagaac
     2821 cacgccgccc gagatgaggt ccgagatcct tatgcccgtg agcgagtcct ggacgtaatc
     2881 gtagagttcc tgggcccggt attccaggat ctggcttata aagctggcgt tgtagtgcct
     2941 gtcgtcgagc ctcacggccg cgacggaatc gatggtgtct gagagcgtcg tcccgtagcg
     3001 gacctttagc tcttcggcct tgtactccgg gatcttcagc ccgtaggcga ggtcggcggt
     3061 gaagctttca ccgccgattg ggatgacgtc ggtgtgggtt agcgccccgc ggtagaagat
     3121 cgctacatcc gtggttccgc cgccgacgtc gagtagcacg acgcccgact cgcggtcggc
     3181 gtccgtcagg caggcctcgg cggacgcgag gggctcgagt acgatcctgg aggccgttac
     3241 cccgcaatcc tcgacggctc gcaggaggtt ctggatgctc gagaccgcgc cgctcacgac
     3301 gtgagctcgc atggttacct tgcgggccgc caggcccacg gggttcttga cgccctccga
     3361 gccatccagc acgtagccgc gggggaccac gtggatcacc tcttcgtcgt cgtccagatc
     3421 aagctgcctt gcttc
//
LOCUS       DBNGVD010000294         3929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4622577, whole genome shotgun sequence.
ACCESSION   DBNGVD010000294 DBNGVD010000000
VERSION     DBNGVD010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4622577"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="AC16CM_07865"
     CDS             complement(<1..1453)
                     /locus_tag="AC16CM_07865"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3139785.1"
                     /translation="MEPVRTEQRPRAIPAPGVNISSLIEKVVAYGPEGTEELVAEAYN
                     AAHAAHRGQIRKSGEPFVYHPLATADVLAELRLDPTTIAAALLHDVLEDTSMTKEELA
                     DRFGDELAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSRDVRVIIIKLADRLHNM
                     RTVAYLKRETQLKKATETLEIYAPLAHRLGIYSIKWELEDLAFATLHPRRYEEIKSLV
                     AARRGDREAFINGTAAELLRHLRETHIEAEVHGRVKHFYSIYNKMVRRNKEFNEIYDL
                     AGLRVVVGDVRDCYGALGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLL
                     EIQIRTQEMDTTAEFGIAAHWMYKHGLTDRQVDRLTWLKSMMEWQRETTDATEFMESL
                     KGELVADEVFVFTPKGDVVSLPSGATPIDFAYHVHTEVGHRTIGARVNDRIVPLDSEL
                     VSGDRVEVITGKNSGPSRDWLAVVQSGRARNKIR"
     gene            complement(1637..3307)
                     /locus_tag="AC16CM_07870"
     CDS             complement(1637..3307)
                     /locus_tag="AC16CM_07870"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3139786.1"
                     /translation="MVDAELAPFRRSGNVRRFSQISRVLVRHGFGFVFDVRRDRREKK
                     GLQEFLAPNFGVRLRRALDDLGPTFVKFGQVLSTRSDILPEGVLSELQRLQDTAKPMP
                     AGVAQRIIERELGAPVDEVFASFDPAPLGSASIGQVHKAVLRSGEIVAVKVQRPEAPS
                     RVAGDLELMRDFAAFLNRRFGQGLFVDVGGLVAEFEVVIRRELDFSAEAENARRFAAN
                     FAGTQVVIPGVYLDLSTSRVLTEEFIEGTRFRDIRPLSMTPSERRRVATMGADAIFKM
                     AFEDGFFHGDPHPSNLLLTPSGDLALLDFGMVGYMSQGDIDALSRLFIAVIQRDASAA
                     LRGLEHLGVGYTTEIRGDLVRDLREFLNKYSGLSVGEVTLGQALSELIALARRYRLRM
                     PPVFPLLTKALVTAEGLARSIDPTINVYEVARPYAQRLLSERFRPRSVAEVVGERAME
                     YARYAEEMPDQVSLIMTEFADGEIEVQLEHGGLDDLLSSVDVLANRVVFAIITAALFV
                     GSAMLGAFDVAGPDVPLTGAPLISFLGFTLALIQTAILLVIIFRSGRL"
     gene            complement(3329..3730)
                     /locus_tag="AC16CM_07875"
     CDS             complement(3329..3730)
                     /locus_tag="AC16CM_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR3139787.1"
                     /translation="MRGTVGDTLDRLILLQIGAAAATRDRVQQIVDGLVEQGRVEREE
                     GRTVVEDVMNRARERSTGARSLVDASVQQGLRRTGVPTKEDYEDVVFRIEQLEHRMRM
                     LEDHPVPPPPPAGEPEMPPASRETPETPPGL"
BASE COUNT          701 a         1296 c         1216 g          716 t
ORIGIN      
        1 ggcggatctt gttgcgggcc cggccactct ggaccacggc cagccagtcg cggctcggac
       61 cggagttttt gcccgtgatg acctcgaccc tgtcccccga gacaagctcg gagtcgaggg
      121 gcacgatcct gtcattcacc ctggccccga tggtgcggtg tccgacctcc gtgtgaacgt
      181 ggtaggcgaa gtctatgggc gtggcgccgc tcggcaaact gaccacgtcg cccttcggcg
      241 tgaacacgaa aacctcgtcg gctactagct cgcccttgag cgactccatg aactccgtgg
      301 cgtccgtcgt ctccctctgc cactccatca tgctcttgag ccacgtcagg cggtccacct
      361 gacggtcggt caggccgtgc ttgtacatcc agtgggccgc gatgccgaac tccgccgtcg
      421 tgtccatctc ctgggtacgg atctggatct ccagcaactt cccctcgttt gacattaccg
      481 tggtgtgaag cgactggtac atgttgaact tgggcatcgc gatgtagtcc ttgaaacgcc
      541 cggggatggg cttccagatg gaatgaataa ccccgagcgc accgtagcaa tcgcgcacat
      601 cgcccaccac cacccgcagg ccggcaaggt cgtagatctc gttgaactcc ttgttgcgtc
      661 gcaccatctt gttgtagatg gagtagaagt gcttgacccg accgtgcacc tcggcctcga
      721 tgtgcgtctc gcgtagatgc cgcaggagct ccgcggccgt tccgttgata aatgcttccc
      781 ggtcaccacg cctggcggcg actagactct ttatctcctc gtaacgccgc gggtggaggg
      841 tggcgaatgc caggtcttcc agctcccact ttatcgagta gatgcccagc ctgtgcgcga
      901 gcggggcgta gatctcaagc gtctccgtag ccttcttgag ctgcgtctcg cgcttcaggt
      961 aggcgaccgt cctcatgttg tgcagcctgt cagccagctt gatgatgatc acccgcacgt
     1021 cgcggctcat ggccacgatc atctttctga gtgactcggc ctgggcctcc tccaggttcc
     1081 cagaagggag ccgcttcagc ttggtgacgc cgtccacgat ctccgcgagc tcgtcaccga
     1141 accgatcggc cagctcctct ttcgtcatgc tcgtatcttc gagcacgtcg tgcaagagcg
     1201 cggcggctat ggtcgtaggg tccagcctca gctccgctag cacgtcagcc gttgcaagcg
     1261 gatggtagac gaacggctcg ccgctcttgc ggatctgacc gcgatgcgcg gcatgggcgg
     1321 cgttataggc ttcggcgacc agctcctcag tcccctccgg cccgtaagct accaccttct
     1381 cgataagaga agagatgttg acgccaggag ccggaatagc cctcggacgt tgctctgtac
     1441 gtacgggttc cacagagaaa ttataccagt cgggttgaag ctatcagctt tcagccttca
     1501 gccatcagct gccgtggtaa gtgatgtaac aacgaggata aatccatacc ttcgacgata
     1561 gcaaccggca atgcgaaccc gacagttcga gccagaacaa acgtagctga aagctgatag
     1621 ctaactgctg acagctctaa agtcgtcccg agcggaaaat gatcaccagc aggatcgccg
     1681 tctggatcag ggccagcgtg aagccgagga agctgatcag gggcgcgccg gtcaggggaa
     1741 cgtcggggcc cgccacgtcg aaggcgccga gcatggcgga gcccacgaac agggcggcgg
     1801 tgatgatggc gaagactacc cggttggcga gcacgtccac gctgcttaac aggtcgtcga
     1861 ggccgccatg ctcgagctga acctctatct ctccgtcggc gaactcggtc atgatgaggc
     1921 tgacctggtc cggcatctcc tcggcgtagc gggcgtactc catcgcgcgc tccccgacca
     1981 cctcggccac gctccttggc ctgaagcgct cggagagcag cctctgcgcg tatggtctgg
     2041 caacctcgta gacgttgatc gtcgggtcta tggagcgggc gagcccctcc gcggtcacga
     2101 gcgccttcgt gagcaggggg aagaccggcg gcatcctgag cctgtagcgc cgcgcgaggg
     2161 cgatgagctc ggagagcgcc tgtcccagcg tgacctcccc gacggagagc cccgagtact
     2221 tgttgagaaa ctcccgcaag tcgcgcacga gatcgcctct tatctccgtc gtgtagccca
     2281 cgccgaggtg ttcgagacct cgcagggcgg ccgacgcgtc gcgctgtatt accgcgatga
     2341 acagcctgga gagggcatct atgtcgccct ggctcatgta accgaccatg ccgaagtcga
     2401 gcagggcgag gtcgccggag ggggtcagga ggaggttgct agggtggggg tcgccgtgga
     2461 aaaagccgtc ctcgaaggcc atcttgaaga tcgcgtcggc ccccatggtg gcgacgcgac
     2521 gccgctccga gggcgtcatc gagagcgggc ggatatccct gaaccgggtc ccctctataa
     2581 actcctcggt cagtaccctg gacgtcgaca ggtccaggta gaccccgggg atgaccacct
     2641 gcgtgcccgc gaagttggct gcgaagcggc gggcgttctc cgcctccgca gagaagtcta
     2701 gctcgcgcct gatcaccacc tcgaactcgg ccacgagccc cccgacgtcc acgaagagcc
     2761 cctgaccaaa cctgcgattc agaaacgccg cgaagtcgcg catgagctcg aggtccccgg
     2821 ccaccctgga gggcgcttcc ggccgctgca ccttgaccgc cactatctcc ccgctgcgca
     2881 acacggcctt gtggacctgc ccgatgctcg cagagcccaa cggcgcggga tcgaaggagg
     2941 cgaacacctc gtcaacgggg gcgccgagct ccctttcgat gatcctctgc gccacgccgg
     3001 ccggcatggg cttggccgtg tcctgcagcc tctgcagctc ggagaggacg ccctccggaa
     3061 gtatgtcaga ccgcgtgctg aggacctggc cgaactttac gaaggtcggg ccgaggtcgt
     3121 cgagggcgcg gcgcagcctg acgccgaagt tcggcgcgag gaattcctgc aggcccttct
     3181 tctccctccg gtcccgcctg acgtcgaaga cgaagccgaa gccgtgacgt acgagcacgc
     3241 ggctgatctg ggagaagcgc ctgacgttcc ccgagcgacg gaacggagcg agctctgcgt
     3301 cgaccaccgg cggcctacgg ggggtagact agagccctgg tggcgtctcc ggggtctccc
     3361 tggatgccgg gggcatttcc ggctctccgg cgggcggtgg cggcgggacg gggtggtcct
     3421 ccagcatccg catcctgtgc tcgagctgtt caattctgaa caccacgtct tcgtaatctt
     3481 ccttcgtcgg aaccccggtc ctcctgagcc cttgctgcac ggaggcgtcg accagcgacc
     3541 gtgcccccgt cgagcgctcg cgggcgcggt tcatcacgtc ctcgacgacg gtgcgtccct
     3601 cttcgcgctc cacgcggccc tgctcgacga gtccgtccac gatctgctgg accctgtctc
     3661 gcgtcgccgc agcggccccg atctgcaaaa ggatcaaacg atccagcgta tctccgactg
     3721 ttccacgcat ggatacctcc ctggagtgag cctcaccacc cacatgataa cgcaagccac
     3781 agaaagagaa cccggcggac ccaagaaacc gggtcaggcc cgctggggtt cccggcgtat
     3841 cgctccggcg cgccacgcct tgtagagcga gagcaccggc gaggcgataa agatcgagct
     3901 gtacgcgccc gccgcgatgc cgaccagca
//
LOCUS       DBNGVD010000295         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4258106, whole genome shotgun sequence.
ACCESSION   DBNGVD010000295 DBNGVD010000000
VERSION     DBNGVD010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4258106"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1025)
                     /locus_tag="AC16CM_07880"
     CDS             complement(261..1025)
                     /locus_tag="AC16CM_07880"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3139788.1"
                     /translation="MAVDVGNTQTVLGLFDGEESLGHWRVATEANRTADELAVFLAAL
                     LALEGLRLDGIGAVIVSSVVPALTRSYRNLASDNLGVPFYVVNAEMETGLDNCYDDPT
                     AVGADRIVNAVAAGKHYGFPAIIVDIGTATTVEAVDGEGCYLGGAILTGLYVSLDALV
                     ERTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAIDALVRRSRDELAEDGVKVI
                     ATGGPAGVIVPYCREIQEFDPDLTLKGLLALYELNT"
     gene            complement(1025..1522)
                     /gene="folK"
                     /locus_tag="AC16CM_07885"
     CDS             complement(1025..1522)
                     /gene="folK"
                     /locus_tag="AC16CM_07885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3139789.1"
                     /translation="MTRTFLSLGSNLGDRLGYLRTATAALGRGPRTAMVATSKIYETE
                     PVEVEGEQPPYLNCVVELECDLPATELLRYCQGVEAALGREMKGERAPRTLDVDVLLF
                     GGETSERPDLTLPHPGVMRAFNRRGLADLDAGLDVPGFGKVGELLGRAESEGISVFGG
                     AEELC"
     gene            complement(1519..>1993)
                     /gene="folP"
                     /locus_tag="AC16CM_07890"
     CDS             complement(1519..>1993)
                     /gene="folP"
                     /locus_tag="AC16CM_07890"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3139790.1"
                     /translation="DPRMAAVVAEAGCPVVLMHMQGEPKTMQSEPRYADVVREVGDFL
                     RSRAEHAVEAGVSRENIIVDPGLGFGKTVDHNLALLRNLKSVVDLGFPVLVGASRKRF
                     IGSITGVEEAAERVFGTVASTVLAYERGATLFRVHDVRANSEALAVAEAVVSTPR"
BASE COUNT          341 a          662 c          633 g          357 t
ORIGIN      
        1 ggggttacga gctcctgatt cctattctcc ataagccgcg agattataag ggcgggtgca
       61 aaaggtgtca acggtcaaag ctgacagcta atagctatca gctgtcagct tctctacggc
      121 aggggaccgt ctctcggaag ggttgcacga cagaataccg cggtgcctgc atggttctag
      181 gacgctacga ggggccgttt tctgttctct cttgctgaaa gctgatggct gaaagccgat
      241 ggctgaaagc tgaccgctct ctaagtgttc agctcgtaaa gggcgaggag tccttttagc
      301 gtgaggtcgg ggtcgaactc ctggatctcg cggcagtacg ggactattac gccggccggg
      361 cctccggtgg caattacctt gacgccgtct tcggcaagct cgtcgcggga acggcggacc
      421 agggcgtcta tggcgccggc gtagccgtag atgaagccgc tacggattga gtcaggggtg
      481 ttggtggcta ttacccttgg cggctcctcc tccaggtcca cgctggggag cttggcggtc
      541 ctctctacca gcgcgtcgag ggagacgtag aggccggtca agatagcgcc gcccaggtag
      601 catccctcgc cgtccacggc ctccacggtc gtggccgtcc cgatatccac gatgatggcc
      661 gggaacccgt agtgcttgcc ggcggccacg gcgttgacga tcctgtccgc cccgacggcc
      721 gtcgggtcgt cgtaacaatt gtcgaggcca gtctccatct ccgcgttcac cacgtagaag
      781 ggcacgccga ggttgtctga ggcgaggttg cggtaggagc gcgtaagggc cggcacgacg
      841 cttgagacga tgaccgcccc gatcccatcg aggcgcaaac cttcgagtgc gagaagagcg
      901 gcgagaaaga cggcgagctc gtcggccgtg cggtttgcct cggtggcgac ccgccagtgc
      961 cccagcgact cctccccatc gaagagcccg agaaccgtct gcgtgttccc aacgtcgacg
     1021 gccatcaaca aagctcctca gcccccccga acacgcttat cccttcgctc tccgcccgcc
     1081 ccagcaactc ccctaccttc ccgaacccgg gaacatcaag gcccgcatcg agatcggcga
     1141 gccccctgcg gttgaacgcg cgcatcaccc cggggtgcgg caacgttagg tcaggtcgct
     1201 ccgaggtctc tcccccgaag agcaggacgt caacgtccag cgtgcgcggg gccctctcgc
     1261 ccttcatctc gcggccgagg gcggcctcga cgccctggca gtagcggagt agctccgtcg
     1321 ccggaaggtc gcactccagt tcgaccacac agttcaggta cggtggttgc tccccttcga
     1381 cctcgaccgg ctccgtctcg tagatcttcg aggtggcgac catagccgtt ctgggtcccc
     1441 tgccgagcgc ggcggtcgca gtgcgcaggt agccgagcct gtcgcccagg ttgctaccga
     1501 ggctcaggaa cgtgcgcgtc accggggagt ggagactacg gcttctgcca cggcgagcgc
     1561 ctcgctgttc gcgcgcacgt cgtggacccg gaagagcgtc gcgcccctct cgtaggcgag
     1621 cacggtggag gccacggtgc cgaagacgcg ctcggccgcc tcctcgacgc cggtgatgct
     1681 ccctataaac cgcttgcgag aggcacccac gagcacgggg aacccgaggt caaccacgct
     1741 cttcagattt cggagcaggg ccaggttgtg gtccaccgtc ttcccgaaac cgagaccggg
     1801 gtcgacgatg atgttctccc ggctcacgcc agcctcgacg gcgtgctcgg ccctggagcg
     1861 gaggaaatcg cccacctctc gcaccacgtc cgcgtaccgg ggctcgctct gcatggtctt
     1921 cggctcaccc tgcatgtgca tcaggaccac ggggcatccg gcttcggcca cgaccgctgc
     1981 catacgggga tcg
//
LOCUS       DBNGVD010000296         1904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2982921, whole genome shotgun sequence.
ACCESSION   DBNGVD010000296 DBNGVD010000000
VERSION     DBNGVD010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2982921"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16CM_07895"
     CDS             complement(<1..533)
                     /locus_tag="AC16CM_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139791.1"
                     /translation="MHLRVGFPLRSEGHLGRAAALASGHPSYHVDGVNGTARVSVDLD
                     LPSDWKLLDDFSGLLRGEAEYATEGSPISADELYGGLRCFLRKQRSGAAAKEWCTPAS
                     LEGKQLFPCRQIRVYDNDHLTANSWYSFGRMDEGAVFSVDKEAITERVLSDLGSCVRC
                     PILDLDATAEIVARLPET"
     gene            complement(677..1315)
                     /locus_tag="AC16CM_07900"
     CDS             complement(677..1315)
                     /locus_tag="AC16CM_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3139792.1"
                     /translation="MREEATGGPLSGRRAEAVRNDRVILDAAREVFVADPKAPISAVA
                     GRAGVGIGALYRRYGSKEDLLRRLAADGLAVYIAVVGAALADEGDPWEAFANFMRRIV
                     DADTHSLTLRLAGTFSPTEELYRDSARAQELNEALFRRTRGAGAIRPDVEVDDIALLF
                     EQLAAVRIGDEERNRQLRRRYLELQLDSLRDSSGSVLPGPPPSWEEIQSRWG"
     gene            1421..1783
                     /locus_tag="AC16CM_07905"
     CDS             1421..1783
                     /locus_tag="AC16CM_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HIR3139793.1"
                     /translation="MDWKLELVAIPVRDVDVAKDFYVEKVGFNADHDHIISDELRFVQ
                     LTPPGSACSIAIGTGIVDSEPGSMRGVQLVVSDVEAARRELVGRGVEVSEVWEGPGGK
                     FVFFGDPDGNRWSVQEIG"
BASE COUNT          338 a          599 c          627 g          340 t
ORIGIN      
        1 gtctcgggaa ggcgggccac tatctcggcc gtcgcgtcga ggtcgaggat ggggcacctg
       61 acgcacgacc cgaggtcgga gaggacacgc tccgtgatgg cttccttgtc cacgctgaag
      121 acggccccct cgtccatcct gccgaatgag taccacgagt tggccgtgag gtggtcgttg
      181 tcgtagacgc ggatctgacg gcacgggaag agctgcttgc cttcgagaga ggccggggtg
      241 caccactctt tggccgccgc cccggaacgc tgcttgcgca ggaaacacct caggccaccg
      301 tagagctcat ccgccgaaat gggacttcct tccgtggcgt actccgcctc gccgcggaga
      361 aggcccgaga aatcgtccag gagcttccag tcagagggga gatccaggtc cacagagacc
      421 cgcgccgtgc cgttgacgcc gtcgacgtgg taggaagggt gccccgacgc gagggccgca
      481 gcccggccga ggtggccttc ggatcttagc gggaaaccga ctctcaagtg catgtcctgc
      541 agattctaac ccacgccacc ggaaaccgtc ggtggcgggt ggtgctagaa gtaggcatca
      601 ggctacaggt ttcaggctgc aggagggtta cctgatgcct ttccccggca ccgcatttct
      661 ccgagaaccg ctccggtcag ccccagcgac tttgtatctc ttcccagctc ggtgggggtc
      721 ccggcaacac cgagccggaa ctatcgcgca gcgaatctag ttgcagctcc aggtaacggc
      781 ggcggagttg cctgtttcgc tcctcgtccc cgatcctgac ggccgccagt tgctcgaaca
      841 ggagggcgat gtcgtcgacc tcgacgtcgg gtcggatggc cccagccccc ctggtgcgcc
      901 ggaagagcgc ctcgttcagc tcctgcgccc tcgcgctgtc acggtacaac tcctccgtgg
      961 gggagaacgt gccggccaga cgcaacgtca gcgagtgggt gtccgcgtcc acgatgcgcc
     1021 gcatgaagtt cgcgaaggcc tcccagggat ccccctcgtc cgcgagcgca gcacccacaa
     1081 ccgctatgta gaccgcaagg ccatcggcgg cgagccgccg cagaaggtcc tccttgctcc
     1141 cgtaacggcg gtagagtgcc cctatcccca ccccggcccg cccggccacg gcggagatgg
     1201 gcgccttcgg atccgccaca aacacctccc gcgcagcgtc caggatcacc cggtcatttc
     1261 gcacggcctc agcacgccgg ccactcaacg gcccccccgt agcttcttca cgcatgccgt
     1321 aattatatgt ggaacagagc gttccgttca agtataatga atcggaacgg aacagtttgt
     1381 tccgcaagag tctcacgggg gtctaggaga gaggagcatc atggactgga agctcgagtt
     1441 ggtggcgata ccggtaaggg atgtggatgt ggcgaaagac ttttacgtcg agaaggttgg
     1501 cttcaacgcg gaccacgacc acatcatcag cgacgagtta cgtttcgtgc agttgacgcc
     1561 tcctggatcg gcctgttcca tcgccatcgg cacggggatc gtggattcgg agcctggttc
     1621 gatgaggggc gtgcagctgg tcgtttcgga tgtggaggcc gcgcgccggg agctcgtcgg
     1681 gcggggggtc gaggtcagcg aggtctggga ggggcctggt gggaagttcg tcttcttcgg
     1741 cgatccagac ggcaaccgct ggtccgtgca ggagatcggc tgacgtagcc gtcgcgtcgg
     1801 agggcacacc gagaggagag tttttttcca tggccgaagt cctcgggcgg cgcgcgctca
     1861 accgcgcgct gctggagcgt cagatgttgt tggcacgcca gaac
//
LOCUS       DBNGVD010000297         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4258979, whole genome shotgun sequence.
ACCESSION   DBNGVD010000297 DBNGVD010000000
VERSION     DBNGVD010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4258979"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="galT"
                     /locus_tag="AC16CM_07910"
     CDS             <1..702
                     /gene="galT"
                     /locus_tag="AC16CM_07910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3139794.1"
                     /translation="HDSTLAGMPERRIRNLVHVWADRYEELGALPFVEYVFIFENKGE
                     AIGVTLDHPHGQIYGFPFIPPRPAEELRAARDYGEHNATACLHCDVLAQEQEDGRRVV
                     VEGEHFTAFVPFYAHFPFEVHVYARRCAPSIAELDADERRDLASVLKRLLTGYDALFG
                     FSLPYMMVMHQAPTDGGDYDGVAHFHIEFYPPNRTGDKLKYLAGSETGAGAFIMDVLP
                     ENSAETLRGKIEEGR"
     gene            699..1673
                     /gene="galE"
                     /locus_tag="AC16CM_07915"
     CDS             699..1673
                     /gene="galE"
                     /locus_tag="AC16CM_07915"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3139795.1"
                     /translation="MRVLVTGGAGYIGSVVASQLAAAGHETVVLDDLSRGHAGAVPEE
                     ATLVRGDLLDAGLVSETLAGGFDGVLHFAALSLVGESVREPELYYRNNVCGTLNLLEA
                     MRESGTGKLVFSSTAAVYGEPESVPIMESDPALPTNPYGSSKLAVDRLIGAVAGAHGL
                     AATSLRYFNVAGASGRYGEDHHPETHLIPLVLRAAAGGTSVKVFGTDYPTRDGTAVRD
                     YIHVEDLGRAHLLALEAAEPGEHSVYNLGNGSGFSVREVVEAARRVTGRDIDAVEAPR
                     REGDPPALVAASDRIRSDLGWEPEKPGLDDMISDAWAWMQDHPRGYDE"
     gene            1685..>2741
                     /gene="galK"
                     /locus_tag="AC16CM_07920"
     CDS             1685..>2741
                     /gene="galK"
                     /locus_tag="AC16CM_07920"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3139796.1"
                     /translation="MRETEQRAARAYEEKFGEAPELVTSAPGRVNLIGEHTDYNGGFV
                     LPCAIDRRVAVAMGAGGGGLYSADFDEMREPGQRDGSWADYPRGVAWAMGEDGRQIRP
                     FQAAFAGDVPLGSGLSSSAAIEAATALAIDSFLGLGIGRKDQAVLCQRAENDFLGVAS
                     GIMDQYASLLCEAGAALLVDCRSLEATSVPLDLPANGLALLVCDTRVERGLADTGYND
                     RRSTCESAAATLGIEHLRDATEDDLDRLSGEQLQRARHVVSENARVLEAVEALNHGDF
                     DLFGKLMYASHASLREDYEVSTPELDTFVEKARDHGALGARLTGAGFGGCAIALIPES
                     RADRVKSACKQDFEAADF"
BASE COUNT          467 a          931 c          916 g          427 t
ORIGIN      
        1 cacgactcga ccctggccgg gatgccggag aggcgcatcc gcaacctcgt ccacgtctgg
       61 gccgaccgtt acgaggagct cggcgccctc ccgttcgtgg agtacgtctt tatcttcgag
      121 aacaagggcg aggccatcgg ggtgacgctt gaccacccgc acggccagat ctatggcttc
      181 ccgttcatac ccccgcgccc cgccgaggaa ttgcgggcgg ccagggatta cggagaacac
      241 aacgccaccg cgtgccttca ctgcgacgtg ctggctcagg agcaagaaga cgggcgtagg
      301 gtggtcgtgg agggcgagca cttcacggcc ttcgttccgt tctacgccca cttcccgttc
      361 gaggtgcacg tgtacgccag gcgctgtgcg ccgtccatcg ccgaactcga cgcggacgag
      421 cgccgcgacc tcgcctccgt cctgaagcgc ctgctcaccg gctatgatgc cctgtttggc
      481 ttctcgctgc cctacatgat ggtgatgcac caggccccga cggacggcgg cgactacgat
      541 ggtgtggccc acttccacat cgagttctac ccgccgaacc gcaccgggga caagctcaag
      601 taccttgcag ggagcgagac cggggccggc gcctttatca tggatgtgct ccccgagaac
      661 agcgcggaga ccctgcgcgg aaagatagaa gagggccgat gagggtcctg gtaaccggcg
      721 gagcggggta catcgggagc gtcgtggcct cccagcttgc ggctgccggc cacgagaccg
      781 tggtgctgga cgatctctcc cggggccacg ccggggccgt accagaagag gcgacgctgg
      841 tcaggggaga cttgctggac gccggcctcg taagcgagac gctcgccggg ggcttcgacg
      901 gcgtgctcca cttcgcggcg ctctcgctcg tgggcgagtc cgttcgtgag ccggaactct
      961 actaccgcaa caacgtctgc ggcacgctca accttctgga ggccatgcgc gaatccggga
     1021 cggggaagct tgtgttctca tccacggccg ccgtgtacgg cgaacccgag agcgtcccta
     1081 taatggagtc ggacccggcc ctgccaacca acccctacgg aagctccaag ctggcggttg
     1141 accgcctcat aggggccgtg gccggcgccc acgggctggc cgcgaccagc ctgcgctact
     1201 tcaacgtggc gggggcgagt ggacgctacg gggaggacca tcaccccgag acgcacctga
     1261 tccccctggt cctccgggcc gccgccggag gcacttccgt aaaggtgttc ggcaccgact
     1321 acccaacccg agacggcacg gccgtgcgcg actacatcca cgtcgaggac ctcggccgtg
     1381 cccacctgct tgcgttggag gcggccgagc cgggggagca cagcgtctac aacctgggca
     1441 acgggtccgg tttctcggtc agggaggtcg ttgaggcggc ccgccgcgtt acgggcagag
     1501 atatagacgc cgtcgaggcc ccgcggcggg agggagaccc cccggcactc gtggccgcaa
     1561 gcgacaggat ccggtccgac ctcggctggg agcccgagaa gccgggcctg gatgacatga
     1621 tctccgacgc ctgggcctgg atgcaagacc acccacgagg ctacgacgag taggagatcc
     1681 caacttgcga gagacggagc agagagcagc cagggcgtac gaggagaagt tcggcgaagc
     1741 cccagagctg gtgaccagcg cgccaggccg cgtgaacctg atcggtgagc acaccgacta
     1801 caacggaggc ttcgtgctcc cctgcgccat cgaccgccgc gttgccgtgg ccatgggggc
     1861 tggaggagga ggcctctact ccgcggattt cgacgagatg cgcgagccgg ggcagaggga
     1921 cggctcctgg gcggattatc cgcgtggcgt ggcctgggcg atgggagagg atggccgcca
     1981 gattcgcccc tttcaggccg ccttcgcggg cgacgtgcca ctcggctcgg ggctgtcatc
     2041 ctcggcggcc atagaagctg ccacggcgct cgccatcgac tcgttcctcg ggctgggtat
     2101 cggccgcaaa gaccaggccg ttctctgtca gcgggccgag aacgattttc tgggcgtcgc
     2161 cagcgggata atggatcagt acgcctccct gctctgcgag gccggggcgg cccttctggt
     2221 ggactgccgc tccctcgaag caacgagcgt ccccctcgac ctcccggcca acggcctcgc
     2281 tctcctcgtc tgcgacaccc gcgtcgagcg tggcctcgcc gacaccggct acaacgaccg
     2341 caggtctact tgcgagagcg cggcagctac gctaggcatc gagcacctgc gtgatgccac
     2401 agaagacgac ctggaccgcc tctctgggga acagctccag cgcgcgcgcc acgtggtctc
     2461 ggagaacgcc cgcgttctcg aggccgtcga ggcgctgaac cacggcgact tcgacctttt
     2521 cggcaagctg atgtacgcct cgcacgcctc ccttcgcgaa gactacgagg tctccacccc
     2581 tgagctcgac accttcgtgg aaaaggccag ggatcacggc gcccttggcg cccgcctgac
     2641 cggcgccggt ttcggcggat gcgccatcgc cctgatcccc gaatccaggg ccgatagggt
     2701 caagagtgcc tgcaagcagg atttcgaagc ggccgacttc g
//
LOCUS       DBNGVD010000298         3798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4259453, whole genome shotgun sequence.
ACCESSION   DBNGVD010000298 DBNGVD010000000
VERSION     DBNGVD010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4259453"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..586)
                     /gene="rfbC"
                     /locus_tag="AC16CM_07925"
     CDS             complement(26..586)
                     /gene="rfbC"
                     /locus_tag="AC16CM_07925"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR3139797.1"
                     /translation="MNVIETGLPGVLIVEPDVFGDARGFFMESWNGARYEEHGIPNTF
                     VQDNLSFSAHGVLRGLHFQNPHPQGKLVSVLEGEVFDVAVDIRVGSPTFGRWTGVTLS
                     AENRRQFWVPPDFAHGFVVTGESALFSYKCTEYYAPEHDGSILWNDPQIGIEWPLQNP
                     SLSGKDGAAPTLDDMPDGRLPRYEKS"
     gene            complement(583..1464)
                     /gene="rfbA"
                     /locus_tag="AC16CM_07930"
     CDS             complement(583..1464)
                     /gene="rfbA"
                     /locus_tag="AC16CM_07930"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR3139798.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPLTTLMLA
                     GIREILVISTPDDLPRFEQLLGDGSGWGVSFEYAVQPEPEGLAQAFVIGRDFVGSDDV
                     SLVLGDNIFFGQGFSGLLERSAARGEGATVFGYYVRDPQRYGVVEFDGDGTVLSIEEK
                     PERPRSHYAVTGLYFYDNRVLDVAAGLRPSPRGELEITDVNVDYLERGELQVELMGRG
                     MAWLDAGTHESLHQSANFIETIEQRQGLKVGCPEEISYRRGYIDAEQLERLAHPMRKN
                     DYGRYLLELLGSEDFVR"
     gene            complement(1461..2540)
                     /gene="rfbB"
                     /locus_tag="AC16CM_07935"
     CDS             complement(1461..2540)
                     /gene="rfbB"
                     /locus_tag="AC16CM_07935"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3139799.1"
                     /translation="MHREKPTILVTGGAGFIGGNFVLESVASGGFEIVNLDALTYAGN
                     PQTLASVADSPDHVFVRGRIGDRELVDRLLVEYRPTAIVNFAAETHVDRSIDGPGAFI
                     QTNVVETHELLEAALAYCGTLEGEEHAAFRFLHVSTDEVYGTLGPTGYFTEESPFRPN
                     SPYAASKAASDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEKLVPLMIMTALRGGSLP
                     VYGDGQQIRDWLFVEDHCKAIRTVLERCVPGETYNVGGHNERTNLEVVRTVCALLDEL
                     APDSPHAPHESLITFVTDRPGHDRRYAIDAGKIERDLGWVPRETLESGLRKTVEWYLE
                     NGEWCAAVLSRGYGGERLGIRGGTA"
     gene            2737..>3798
                     /gene="thrC"
                     /locus_tag="AC16CM_07940"
     CDS             2737..>3798
                     /gene="thrC"
                     /locus_tag="AC16CM_07940"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3139800.1"
                     /translation="MRFVLGVGARVVRQGVIGRYRGWLPEIPDGAIASIGEGGTPLVE
                     ATTISERVGARLFLKFEGMNPTASFKDRGMTVAMSRAVAEGSRACVCASTGNTAASAA
                     AYAARAGVECFVVVPAGKIALGKAVQVLAHGARIVQIDGNFDAALRLSQRIAEELGDV
                     TLVNSVNPDRIEGQKTAAFEVAEALGGSPDALALPVGNAGNITSYWKGFREWREAGHT
                     ESVPRMLGFQAEGASPLVAGHDFENPETVASAIRIGSPASKEGALAAARDSGGAIESV
                     TDAEILAAQDLLAREEGVFCEPASAAGVAGLLKLADEGRAPDGTVVSVLTGHGLKDPD
                     IVLGRVRLPDPVPATFEAVT"
BASE COUNT          620 a         1227 c         1289 g          662 t
ORIGIN      
        1 aagggaccgg cagacccgcc cgtgctcatg atttctcgta gcgcggcagc ctgccgtccg
       61 gcatgtcgtc tagcgtgggg gcggcgccgt ccttgccaga gagggagggg ttctgcaggg
      121 gccactctat ccctatctgc gggtcgttcc acaggatgga cccgtcgtgc tcgggggcgt
      181 agtattcggt gcatttgtag gagaagaggg cgctctcgcc ggtcacgacg aagccgtgtg
      241 cgaagtcggg gggcacccag aactggcgcc tgttctcggc cgagagggta acgccggtcc
      301 accggccgaa agtgggagag ccgaccctga tgtccacggc cacgtcgaag acctcgccct
      361 cgagcaccga gacgagcttg ccctgggggt gggggttctg gaagtgtagc ccgcgcagca
      421 cgccgtgggc tgagaaagag aggttatcct gcacgaaggt gttggggatg ccgtgctctt
      481 cgtagcgggc cccgttccag ctctccataa agaaaccgcg ggcgtcgccg aacacgtcgg
      541 gctcgacgat caggacccct ggaagccccg tctcgataac gttcacctca cgaaatcctc
      601 gctgccgagc agctcgagga ggtaacggcc gtagtcgttc ttcctcatag ggtgggcgag
      661 gcgctcgagc tgttcggcgt cgatgtagcc cctgcggtag gagatctcct cggggcagcc
      721 caccttgagg ccctgccgct gctcgatggt ctcgatgaag ttggccgact ggtgcaaaga
      781 ctcgtgcgtg ccggcgtcga gccaggccat cccccgtccc atgagctcga cctgcaactc
      841 cccgcgctcc agatagtcca cgttgacgtc cgttatctcc agctcccccc gcggcgatgg
      901 ccggaggcct gcggccacgt cgagcaccct gttgtcgtag aagtagagcc ccgtcacggc
      961 gtagtgcgac cggggccgct ccggtttttc ctcgatgctg agcaccgtgc catcgccgtc
     1021 gaactcgacc accccgtagc gctggggatc acggacgtag tacccgaaga ctgtggcacc
     1081 ctcgcccctg gcggccgacc gttcgaggag cccggagaag ccctgcccga agaagatgtt
     1141 gtcaccaagc acgagcgaga cgtcgtcgga gccaacgaaa tccctcccga taacgaaggc
     1201 ctgggcaagc ccctccggct cgggctgcac ggcgtactcg aagctcacgc cccacccgga
     1261 cccgtcgcca agcagttgct cgaagcgcgg caggtcgtcg ggggtcgaga tcacgagtat
     1321 ctccctgatg ccggctagca tgagcgtggt cagggggtag tagatcatgg gcttgtcgta
     1381 gacgggcagc aactgcttgc tgaccgccct ggtgagcggg tagaggcgtg tcccagaccc
     1441 cccggcgagc acgatgccct tcacgccgtc cccccacgga tgcccagccg ctccccaccg
     1501 tagccccgcg agagaacggc cgcgcaccac tccccgttct ccagatacca ctcgaccgtc
     1561 tttctaaggc cactctccag cgtctctctc gggacccagc cgaggtccct ctctatcttg
     1621 ccggcgtcta tggcgtagcg tctgtcgtgg cccggcctgt cggttacgaa ggtgatgagc
     1681 gactcgtgcg gcgcgtgcgg cgagtcgggg gccagctcgt cgagcagggc gcagacggtc
     1741 ctgacgacct ccaggttcgt ccgctcgttg tgcccgccca cgttgtacgt ctcgcctggc
     1801 acgcaacgct cgagcaccgt gcggatagcc ttgcagtggt cctccacgaa gagccagtcc
     1861 ctgatctgct gcccgtcgcc gtagaccggc agggacccgc cgcgcagggc ggtcatgatc
     1921 atgagcggga ccagcttctc cgggaaatgg tagggcccgt agttgttgga gcagttggtg
     1981 gtcagggtgg gcaggccata agtatgatgg taggcccgga ccagatggtc tgaggccgcc
     2041 ttggaggccg cgtagggcga gttcggccgg aaggggctct cctcggtgaa gtagcccgtc
     2101 ggcccgagcg ttccgtacac ctcgtccgtg gagacgtgaa ggaagcggaa cgcggcgtgc
     2161 tcctctccct ccagcgtgcc acagtaagcc agcgcggcct ccaacaactc gtgcgtctcg
     2221 accacgttcg tttgtatgaa cgctcctggc ccgtcgatgg aacggtccac gtgcgtctcc
     2281 gcggcgaagt tcacgatcgc cgtcggccgg tactccacga gcagacggtc aaccagctcc
     2341 cggtccccga tcctaccccg cacgaagacg tggtccgggc tatcggcaac agacgcgagc
     2401 gtctgggggt tacccgcgta ggtcagagca tccaggttca cgatctcgaa cccacccgag
     2461 gccaccgact ccaggacgaa attccccccg atgaacccgg ccccgccggt tacgaggatc
     2521 gtgggtttct ctctgtgcaa agcgctcctc ggtgcatagc tgtcagcttt tagccgtcag
     2581 ctttcagctt gttgatctct ggctgaccgc tgatcgctga aggctgatag ctctttacag
     2641 cctactagaa tcgtcctgtt gttgaaaggc tgcgcttttg cgtgataggt ttgttctttc
     2701 ggcctggaag caggggccgg cacgggttac ggagagatga ggttcgtatt gggcgtcggg
     2761 gcgcgggttg tgcggcaggg tgttatcggg aggtatcggg ggtggttgcc cgagataccc
     2821 gatggggcca tcgccagcat cggggagggt gggacgccgc ttgtcgaggc tactactatc
     2881 tcggagcgcg tgggggcgcg gctgttcttg aagttcgagg ggatgaaccc gacggcctca
     2941 ttcaaggacc gaggcatgac ggtggccatg agccgggccg tcgccgaggg gagtcgggcc
     3001 tgtgtttgcg cctcgacggg caacacggcg gcctcggctg cggcctacgc cgcgagggcc
     3061 ggcgtggagt gcttcgtggt cgtgccagca ggcaagatcg cgctcgggaa ggccgttcag
     3121 gtcctcgcac acggggccag aatagtccag atcgacggca acttcgacgc ggccctgcgg
     3181 ctctcgcaga ggatcgccga ggagctcggc gacgtgacgc tggtcaactc ggtcaacccg
     3241 gaccggatcg agggccagaa gacggcggcc ttcgaggtag ccgaggctct cggcggttcc
     3301 cccgatgcgc ttgccctgcc tgtgggcaac gcggggaaca tcaccagcta ctggaagggt
     3361 ttccgcgagt ggcgggaggc cgggcacacc gaatcggtcc cacgcatgct cgggttccag
     3421 gccgagggcg cctcgccgct tgtggccggc cacgacttcg agaaccccga gacggttgcg
     3481 agcgccatca ggatagggtc gccggccagc aaggagggcg ccctcgccgc ggcgcgcgac
     3541 tccggcggcg ccatcgagag cgtcacggac gcggagatcc tggcggccca ggaccttctc
     3601 gcccgggaag agggggtctt ctgcgaaccg gcctcggcgg cgggcgtggc cgggctcctc
     3661 aagctggccg acgagggcag ggcccccgat ggcacggtcg tgagcgtcct gaccggccac
     3721 gggctcaagg accctgacat cgtgctgggc cgcgtgcggc tccccgatcc cgtccccgcc
     3781 acgttcgagg ccgtaaca
//
LOCUS       DBNGVD010000299         4420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2254825, whole genome shotgun sequence.
ACCESSION   DBNGVD010000299 DBNGVD010000000
VERSION     DBNGVD010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2254825"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1165)
                     /gene="adhP"
                     /locus_tag="AC16CM_07945"
     CDS             complement(77..1165)
                     /gene="adhP"
                     /locus_tag="AC16CM_07945"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HIR3139801.1"
                     /translation="MKAAVVHDFSEPLSLEEVPKPEPAEDEVVVEVEASGLCHTDIHA
                     AHGDWPVKPKLPLIPGHEGVGIVESVGKNVTEVREGDRVAVPWLGYACGACEYCASGW
                     ETLCEHQLNTGYGIDGGHAEYVKANARFVGRVPGGIHPFEAAPLTCAGVTTYKAVKVS
                     GARPSDLVAVFGVGGLGHLAVQYARIAGAAVVAVDLFDDKLRLARELGADYAVNAREQ
                     DPIEEIRKLGGADAVIATAVSPRAFEQGFGSLKRGGTLTLVGLPEDNEMRLPIFETVL
                     GGIHVTGSIVGTRVDLQEVFELHAMGRTRVVHEERKLEDVNEAIAEVESGEVDARLVF
                     DLGTERPARRSEGVFTEERRSASEQPTG"
     gene            complement(1428..2333)
                     /locus_tag="AC16CM_07950"
     CDS             complement(1428..2333)
                     /locus_tag="AC16CM_07950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139802.1"
                     /translation="MRNTALVLASMTLAGLMACMAAVFAAVPGDAQTSTTTLVGAGDI
                     AGCSQTNDSATARLLGRIEGTVFTLGDNVQGQGTRSEFRNCYDPTWGKYKNRTRPSAG
                     NHDYNTEGAIPYFDYFGWRAEPRRGYYAYQRGSWHIVALNSNCDRVRVGGCGRRSPQG
                     RWLRNNLDNHPARCTIAYFHHPLYATGKNTTSTKVRPLWEILYNHRADIILSGHAHRY
                     ERYAPIRPGGVVDQQNGIRQFVVGTGGATDGSEIYRGQAPGLQVVKLNTPGVLKLDLG
                     VGSYRWKFVPMAGKTFTDSGTDECH"
     gene            complement(2418..2915)
                     /locus_tag="AC16CM_07955"
     CDS             complement(2418..2915)
                     /locus_tag="AC16CM_07955"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3139803.1"
                     /translation="MPDEQAKAFVRRQIDELWNRGNLDFAVECFTEDFVGHDPASPEG
                     VHGPEGFKQNVATTRAAFPDFHVEIVDQVAEGDRVVTRYVTTGTQQGEVGGLPPTGRR
                     VAVAGMGIDYFREGKICESWEYYDVMGTMQQLGMIVVPGPLLLARTLVHQARKLRTRS
                     LGGDR"
     gene            complement(3064..3198)
                     /locus_tag="AC16CM_07960"
     CDS             complement(3064..3198)
                     /locus_tag="AC16CM_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139804.1"
                     /translation="MEFENKPGSNAPPRVGERVMVLYEPQRPEEAKVAFDCIVRRKTD
                     "
     gene            complement(3294..3434)
                     /locus_tag="AC16CM_07965"
     CDS             complement(3294..3434)
                     /locus_tag="AC16CM_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139805.1"
                     /translation="MASDGYSTFIAGLEAMFGERIGTLGLGEVERRRFPQASAITRGR
                     GR"
     gene            complement(3480..3779)
                     /locus_tag="AC16CM_07970"
     CDS             complement(3480..3779)
                     /locus_tag="AC16CM_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139806.1"
                     /translation="MRGSPEGDGDGCIVAAPGGPVALGSGASGALAANGPSSVLAGLV
                     GSFTARGIDLFASAAAVVIVRSDTEDAAASSVGVGYTLAGDVIRALPEALAPLSD"
     gene            3972..>4420
                     /locus_tag="AC16CM_07975"
     CDS             3972..>4420
                     /locus_tag="AC16CM_07975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3139807.1"
                     /translation="MKAIVYTEYGPPDVLQIREVEKPAPREDEVLVKVHAASANPADW
                     HLMRAEPFLARIANGLLKPKNTRLGADLAGRVEVIGRNVTQFRAGDDVFGELSLDELG
                     AFAEYTCAPEELLALKPAMLTFEQAAAVPLAAITALQGLRDKGQIQPG"
BASE COUNT          760 a         1470 c         1325 g          865 t
ORIGIN      
        1 ggacgcctcg gatggacccg aggcgtcctt cgtgctggtg ctggcgaccc gcgatcgggc
       61 ccttccccac cccggtctag ccggtgggct gctcggaggc gctacgccgc tcctccgtga
      121 agacgccttc gctccggcgt gctggtcgct cggtgccaag gtcgaagact agcctggcat
      181 ccacctcgcc cgactcgacc tccgcgatgg cctcattgac gtcctccagc ttcctctcct
      241 cgtggacgac cctcgtcctg cccatcgcgt gaagctcgaa gacttcctgc aggtccaccc
      301 tggtccccac gatagagccc gtaacgtgga tgccgcccag aaccgtctcg aagatcggca
      361 accgcatctc gttatcctcg ggaagcccca cgagggtgag cgtgcccccc cgtttgagcg
      421 agccaaaccc ctgctcgaac gcccttgggg agacggccgt ggctataacg gcgtcggcac
      481 ctccgagctt ccggatctcc tcgatgggat cctgttcgcg ggcgttgacg gcgtagtccg
      541 ccccgagctc tctggcgagc cggagcttgt cgtcgaagag gtcgacagcg accacggcgg
      601 cgccggctat cctggcgtac tggacggcca ggtggccgag gccaccgacg ccgaaaaccg
      661 cgaccaggtc ggacggcctt gcccccgaga ccttcaccgc cttgtacgtg gtcacccccg
      721 cgcaggtcag cggggcggcc tcgaaggggt gaatcccgcc tggaacccgt ccgacaaacc
      781 tggcgttggc tttgacgtac tcggcgtgtc cgccgtcgat cccgtatccg gtgttgagtt
      841 gatgctcaca cagggtctcc cagccggagg cgcaatactc gcaagccccg caggcgtagc
      901 cgagccaggg cacggccacc cggtcgccct ccctgacctc ggttacgttc ttgcccacgc
      961 tctcgacgat accgacgcct tcgtggccgg gaatcagggg cagcttcggc ttgaccggcc
     1021 aatcaccgtg ggcggcgtgg atatcggtgt ggcagaggcc ggaggcctcc acctcgacga
     1081 ccacttcgtc ctcggcgggt tcgggtttgg gcacctcctc gagcgagagc ggctcggaaa
     1141 aatcgtgtac caccgcagcc ttcatggatc acgcctcctc aggctcgttt ctggttcacc
     1201 cgctgtccgc cacgccatcc ctctagatat acctggatgg gcgctcggga tcattgcgac
     1261 aggctacccc agggcaggac cctttccctg atcagaattg accaaccggg aagacaacct
     1321 tgaccagagt cggcggtgct cgccggcatc tttatgcgcc gccacaacat gtagcgtcga
     1381 atcgtttgcc tccaggggcc gggggtgaac aggggcgatg gcacacccta gtgacactcg
     1441 tcggttcccg agtcggtgaa cgtcttgccc gccatgggga cgaatttcca cctgtaggaa
     1501 ccaaccccga ggtctagctt caggacgccc ggcgtgttga gcttcacaac ctgcagccct
     1561 ggggcctggc cgcggtagat ctcactgccg tccgtggcgc ctccggtccc taccacgaac
     1621 tggcggatgc cgttctgctg gtctaccacg ccccctggac gtatcggagc gtagcgctcg
     1681 tagcggtggg cgtgcccgga gaggatgatg tctgctctgt ggttgtagag gatctcccac
     1741 aggggcctta ccttggtcga ggtggtgttc ttgcccgtgg cgtagagcgg gtggtggaag
     1801 taggctatgg tgcacctggc ggggtggttg tcgaggttgt tcctgagcca gcgcccctga
     1861 ggagagcgcc ttccgcagcc gccaaccctt accctgtcgc agttgctgtt tagggccact
     1921 atgtgccagc tgccgcgttg gtaggcgtag tatcctctcc tgggctccgc ccgccatccg
     1981 aagtagtcga agtagggtat cgccccttcg gtgttgtagt cgtggttgcc tgcggagggt
     2041 ctggtgcggt tcttgtactt gccccaggtt gggtcgtagc agttcctgaa ctcgctgcgg
     2101 gtgccctggc cctgcacgtt atccccgagg gtgaagacgg tcccctctat cctgcccaat
     2161 agcctcgccg tggcgctgtc gttggtctgg gagcagccgg ctatgtctcc agcccccacc
     2221 aacgtcgtgg tactggtctg cgcgtcgccc ggaactgccg caaacacggc agccatacat
     2281 gccatcaggc ccgccagagt catcgaagcg agcaccagag cggtgtttct catctcctcc
     2341 ccttccataa cagagagcct ctacaaggct acagagccgc acgagggcag taggatgcca
     2401 cgttacgaag gcccctacta cctgtcccct cccaaagatc tagtacgcaa cttcctcgct
     2461 tgatggacca gcgtgcgtgc aagtagcagt gggcctggca cgactatcat gcccagttgc
     2521 tgcatcgtgc ccataacgtc gtagtactcc cacgactcgc agatcttgcc ctcccggaag
     2581 tagtcgatgc ccatcccggc caccgcgacc cgcctgccgg tcggggggag gcccccaacc
     2641 tctccctgtt gggtgccggt ggtcacgtaa cgtgtcacca cccggtcccc ctcggcaact
     2701 tggtccacta tctcgacgtg gaaatcgggg aaggccgccc gagtcgtggc gacgttttgt
     2761 ttgaaccctt ccgggccatg caccccttct gggcttgcgg ggtcgtggcc cacgaagtct
     2821 tcggtgaagc actccacggc gaagtcgagg ttgcccctgt tccacaactc gtctatctgg
     2881 cgtcgcacga aagccttcgc ctgttcgtct ggcatggcgc ttcctccttc ctatcgaggc
     2941 cggctacgca taacgctgtc tagccgatct accgccggcc cgatcccata ccgcatccca
     3001 accctagcct cggcacggct caccttcttg aaacaagttg attatttctc tagctactgt
     3061 agatcaatct gtcttgcgcc tgacaataca gtcaaaggcg acctttgcct cctcgggccg
     3121 ctggggctcg tagaggacca taacccgctc gccgacccgt ggaggcgcgt tgctgcctgg
     3181 cttgttctcg aactccaccg tctagccgtc agcagtttgg aaccgcacgg aagggaaaaa
     3241 cacccgcccg gggaggtcgc tcacgtgatg ggtgtgtctg atcatccctg gagctatctc
     3301 cctctacctc tcgtgatggc ggaggcctgg gggaagcgtc tgcgctcgac ctcgcccagg
     3361 ccgagcgtcc cgatcctctc cccgaacatc gcttcgagtc cagcgatgaa cgtagaatat
     3421 ccatcgctgg ccacctgccg atcctcgtag ttcaattctc cggccctgag cgacggcgcc
     3481 tagtcgctca ggggagccag cgcctcggga agcgcccgga taacatcccc cgccagcgtg
     3541 taacccacgc ctacgctgga cgcggcggca tcctcggtgt cggaccgcac gatcaccacc
     3601 gccgcggcgg acgcaaacag gtctattccc cgcgctgtaa acgacccgac gagccccgcc
     3661 agcacgctac tgggcccatt ggctgccagg gcgccgctcg cgccggatcc gagggctacc
     3721 gggccgccgg gtgcagcgac gatacacccg tctccgtctc cctctggcga cccgcgcata
     3781 ggtgctgacg ttgcggttgc ggctgtccga aacctcggcc gtgaccgtcg gtagcctgga
     3841 gtctttcctg cgagacatgg cggcagggct agtgagcgcc atttctggga tccacggaga
     3901 gtatgatctc gacaaccagt gcggtccctc gagaccgcgc caatgcaaac ctggaggaaa
     3961 ggaggaagct catgaaagcg attgtctaca cagaatacgg accaccggat gttcttcaga
     4021 tcagggaagt ggaaaaacct gcgcccaggg aagacgaagt attggtgaag gtccatgcgg
     4081 catccgcgaa tccagccgac tggcatctta tgcgagcaga gccattcctg gcacgaatag
     4141 cgaatgggct tctgaagccc aagaacacga ggctcggcgc tgatctggcg gggcgtgttg
     4201 aagtgattgg ccgcaacgta actcagtttc gagcaggtga tgatgtattt ggcgaactgt
     4261 ccctggatga gttgggcgcc tttgccgagt atacctgtgc tcctgaagag ctactggcgt
     4321 tgaaaccggc catgttgaca ttcgagcaag cagcagccgt ccctctagcc gccatcaccg
     4381 ctttgcaggg tctacgcgac aaagggcaga ttcagccagg
//
LOCUS       DBNGVD010000300         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_798531, whole genome shotgun sequence.
ACCESSION   DBNGVD010000300 DBNGVD010000000
VERSION     DBNGVD010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_798531"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16CM_07980"
     CDS             <1..663
                     /locus_tag="AC16CM_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HIR3139808.1"
                     /translation="TRTQKTPFHLEKELRLRSGESRLLVEETLKNESEVPLHAMWGHH
                     IAFGRPFLEDGCRIRLPEGATVVLHDEPIHPEGRRIKGGRTYAWPIVEDQAGASVDLS
                     VLPEAGTMSEMLYLKDLSEGWYEVENPKKALALRVEWDVERMPYLWFWQEYGASGFYP
                     WYGRHYNIGLEPFSSYPTNGIAEAVSNGTAMLVGAREERRFSMGVEVLEGSEGGEGGE
                     HG"
     gene            656..1447
                     /locus_tag="AC16CM_07985"
     CDS             656..1447
                     /locus_tag="AC16CM_07985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139809.1"
                     /translation="MADLIGKVGIVTGALGIGLAAARKLASDGASVVVCSDREEQVEE
                     AVTALHEEALEARGLRANVTSPADMEGLVGFAAESYGGVDILVNAAGVQRYGTVEDTV
                     EEMWDEVLGVNLKGVYLASKYVIPQIRKRGGGSIVNVSSVQAFASQKGVAAYTASKGG
                     VNALTRAMALDHAAENIRVNAVCPASVDTPMLRWSAELFKGEKTAEETLEGFGKMHPL
                     GRVARAEEVAEVIAFLAGPRASFVTGGEYKVDGGMLAALGVQLPE"
     gene            1470..2711
                     /locus_tag="AC16CM_07990"
     CDS             1470..2711
                     /locus_tag="AC16CM_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3139810.1"
                     /translation="MKITDLRATTVTVPLEASLLHANGAHWGRFVRTIVELETDEGLI
                     GLGEMGGGGESTEVAFEGLKPYLLGHDPSQLEAMRFKIMNPTASLYNNRTQLHAALEF
                     ACLDLVGQKLGVPVHDLLGGRLRDEVSFASYLFFRYADPETGEGEVRTAEQLVEHARA
                     LKEEHGFAAHKLKGGVYPPEYELECYRALAEAFPGDTLRYDPNAALSVEEGARFGRAI
                     EDLDNDYYEDPTWGLNGMRRLRGMVNIPLSTNTVVIDFEQLAANILDPAVDVILLDTT
                     FWGGIRPCMKAAGVCETFQLGVAVHSSGELGIQLATMLHLGAAIPNLSFSADAHYHHL
                     RDDIIEGGKLEYRGGAIRVPDTPGLGVRLDRDRLEHYAELYKELGGYPYDRDPGHPGG
                     YALVPKQRFADPSTSLKPDLR"
     gene            complement(2891..>3178)
                     /locus_tag="AC16CM_07995"
     CDS             complement(2891..>3178)
                     /locus_tag="AC16CM_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139811.1"
                     /translation="VGGYWTRKGDVEVDLVGAEEKARPRRVAFVGSIKWRERTPFGGR
                     DLGRIAAQRDEVPGTDKDTVLIGVSREGFDEQGSSLDAALVPTDLLEAWKQ"
BASE COUNT          621 a          969 c         1056 g          532 t
ORIGIN      
        1 accagaactc agaaaacgcc cttccatcta gagaaagagc ttcggctacg ctccggagag
       61 agccgcctcc tcgtggagga gacgctcaag aacgagagcg aggttcccct gcacgccatg
      121 tggggacacc acatagcgtt cgggcgtcct tttctcgagg acggctgcag gatacgtctt
      181 cccgaagggg cgacggtagt gctgcacgat gagccgatcc accccgaagg acgccgtata
      241 aagggcggca gaacctacgc ctggcccatc gtcgaagacc aagcgggtgc cagcgtggac
      301 ctgagcgtgc tgccggaggc aggaactatg agcgagatgc tctacctcaa ggacctctca
      361 gagggctggt acgaggtgga gaacccgaaa aaggctctgg cgctccgagt cgagtgggat
      421 gtcgagcgga tgccgtattt gtggttctgg caggagtatg gcgcgtcggg attctacccg
      481 tggtacggac gccactacaa cattggccta gaacctttct caagctaccc gacgaacggt
      541 attgcagagg cggtgagcaa cggcaccgcc atgctcgtgg gcgcgcggga agaacggcgt
      601 ttctctatgg gggtcgaggt tctggagggc tcagagggtg gagaaggagg agaacatggc
      661 tgatctcatc ggcaaggtgg gaatcgtaac cggcgccctg ggcatcggtc tggccgcggc
      721 acgcaagctg gcgtcggacg gcgcctcggt cgtcgtttgc agcgaccgcg aggaacaggt
      781 agaagaggcg gtaacggccc tacacgagga ggccctggag gctcgtggcc tccgggccaa
      841 cgtgacctcc ccggccgaca tggaagggtt ggtgggcttc gctgccgaga gctacggggg
      901 ggtcgatatc ctcgtcaacg ccgccggcgt tcagcgctac gggaccgtag aggacaccgt
      961 ggaggagatg tgggatgagg ttctcggcgt gaacctcaag ggcgtttacc tggcgtccaa
     1021 gtacgtcatc ccccagatca ggaagcgcgg gggaggctcg atcgtcaacg tctcgagcgt
     1081 gcaggcgttc gcctcgcaga agggggtcgc cgcgtatacg gccagcaagg gaggcgtcaa
     1141 cgccctgacc cgggcgatgg cgctggatca cgccgcggag aacataaggg taaacgcggt
     1201 ctgcccggcc tccgtggaca cgccgatgct gcggtggtcg gccgagcttt tcaaggggga
     1261 gaagacggcg gaagagacgc tggagggctt cggcaagatg cacccgctcg ggcgggtagc
     1321 cagggcggaa gaggtggcag aggttatcgc cttcctcgct ggtcctaggg ccagctttgt
     1381 taccggcggc gagtacaagg tggacggcgg catgctggcc gcgctcggcg tccagttgcc
     1441 agagtgaggg cgatcgggaa aggagttgca tgaagatcac cgacctgcgc gcgaccaccg
     1501 tcacggtacc actggaggcg tcgcttctgc acgccaacgg cgcccactgg ggccgcttcg
     1561 tccgcaccat agtcgagttg gagaccgacg agggccttat cggcctgggc gagatggggg
     1621 gcggaggaga gagcacggag gtggccttcg agggcctcaa accgtatctc ctcgggcacg
     1681 atccctccca gctcgaggcg atgcgcttca agatcatgaa cccgacggcg agcctctaca
     1741 acaaccgcac ccagctgcac gccgcgctcg agttcgcctg cctggacctc gtgggacaga
     1801 aactcggggt ccccgtccac gacctgctcg ggggcaggct tcgcgacgag gtgtccttcg
     1861 ccagctacct gttcttcaga tacgccgatc ccgaaacggg cgagggcgag gtacggaccg
     1921 cggaacagct cgtcgagcac gcccgggccc tcaaggaaga gcatggtttc gccgcccaca
     1981 agcttaaggg tggggtctat ccgcccgagt acgagctgga gtgctacagg gcgctggccg
     2041 aggcatttcc tggagacacc ctacgctacg atccgaacgc agccttgagc gtcgaggagg
     2101 gcgcccgatt cggacgggcc atagaggacc tcgacaacga ctactacgaa gacccgacct
     2161 ggggcctgaa cgggatgcga cgtcttcgcg ggatggtgaa catacccctc tccaccaaca
     2221 ccgtcgtgat cgatttcgag cagctcgccg ccaacatcct cgacccggcc gtggacgtga
     2281 tactgcttga caccaccttc tggggcggca taaggccatg catgaaggct gcaggggttt
     2341 gtgagacatt ccagctcggg gtggccgtcc attcctctgg agagctgggg attcaactcg
     2401 cgacgatgtt gcacctgggc gcggcgatcc cgaacctgtc cttctcggct gacgcccact
     2461 accatcacct tcgcgacgac atcatagagg gcggaaagct cgagtaccgt ggaggagcca
     2521 tccgggtccc cgacacccca ggactcggag tccgtctgga ccgggacagg ctggagcact
     2581 atgcggaact gtacaaggag ctgggagggt acccctacga ccgagacccg ggccaccccg
     2641 gcggctacgc cctggtaccg aagcaacgct tcgcagaccc cagtacgagc ctcaagccgg
     2701 atcttcgttg aatagagcat cgcgcgagaa gaagccctac gctactctct tggtaggaca
     2761 accaggtggt gagggtcaat gaggtattcg gtagtgtttc tttctgcgag accgtcggta
     2821 gctgagaagg tctaggaatc agaaggccgc aggttcgagt cctgccgagc gcgctctcag
     2881 aagccccgta tcactgcttc caggcttcca gcaggtccgt gggaacgagg gccgcgtcga
     2941 ggctactgcc ctgctcgtcg aatccctctc tggagacgcc gataaggacg gtatctttgt
     3001 ccgttcctgg cacctcgtcg cgctgcgccg cgatgcgacc caggtcgcga ccgccgaagg
     3061 gcgttctctc acgccatttg attgagccca cgaacgcgac gcgcctcgga cgcgccttct
     3121 cctccgctcc gacgaggtcc acctccacgt cccccttcct cgtccaatac ccgcccac
//
LOCUS       DBNGVD010000301         3152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1890737, whole genome shotgun sequence.
ACCESSION   DBNGVD010000301 DBNGVD010000000
VERSION     DBNGVD010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1890737"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16CM_08000"
     CDS             <1..534
                     /locus_tag="AC16CM_08000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139812.1"
                     /translation="ERLILRRFRDSDLAAFVAYRNDPEVARYQSWDSIDELEARALIR
                     EMASARPGVPGDWFQFAIESKETGALLGDCALQVDGQEPYKAELGFTLAREHQGKGFA
                     SEAASRLLDYAFASLDLHRVFALADCRNRPSWTLLDRVGMRREGHFLENVWFKGEWCD
                     EYLYAVLKDEWLDRPTG"
     gene            complement(531..2030)
                     /gene="xylB"
                     /locus_tag="AC16CM_08005"
     CDS             complement(531..2030)
                     /gene="xylB"
                     /locus_tag="AC16CM_08005"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3139813.1"
                     /translation="MSVLVGLDVGTGGARAVAVDGSGKIVAEAGSEFPLHTPRPGWTE
                     QDPEDWWTGAKEVLARVASEASEEVAGIGLTGQMHGSVFLDSSDEVVRPALLWNDQRT
                     QAQCDRITEAVGAERLISIAGNPALTGFQAPKIVWLRDEEPEEYGRVSRVLLPKDYVR
                     LMLTGEHATDASDASGTLLLDVRARDWSGEILDALEIPHEWMPKVYEGPENTGALRES
                     VAEELGLLPGIPVAAGGGDNAAAAVGTGIVEPGLVNSSVGTSGVLFAHTEEFKPDPSG
                     RLHAFCHAVPGAYHLMGVTLSAGGSLSWWRETLGGDFDELVEEASNVPPGSEGLVFLP
                     YLSGERTPHLDPKARGSFFGLTARHGVAHMTKAVMEGVVFSLRDSLQVMRDLDVPVED
                     VRATGGGARSGLWRQLQADIYGTPIRRTVADEGPAYGAALLAGVASGTYTDVEEATSV
                     VELREELTEPDAGRSKAYGEYYEVYRSLYPATGPVMARLSDLAAGTSDG"
     gene            complement(2027..3106)
                     /locus_tag="AC16CM_08010"
     CDS             complement(2027..3106)
                     /locus_tag="AC16CM_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3139814.1"
                     /translation="MIKNSVGIWAFGPAVTRFVPPGYHHEVADEPMTDKTERVAGGLN
                     DLLDGLEYHFPGEINEDNVDEVTRILASHEMDLPVIAAGLHPDPTYALGAFVNPDEGL
                     RRQGIDTLRRGIDLCAQIGANFIIWPGAEGYNYTFQRPYAQTWRRFVEGVAELTDHAN
                     ERNVKVFLEHKNSEPAMKILMQNIGMTLFTIQKVNALGVDTTNLQVNMDWQHLIMNGE
                     NLAEYADLLASEHKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDVGYGEGGE
                     IVGFDLYPYTEDQIAAVRRAILQWEFIWDLARKIDRGALDGARDRADALAGQREVYRV
                     LGMDEDYEVEVGRRRREARAGRSQA"
BASE COUNT          497 a         1031 c         1046 g          578 t
ORIGIN      
        1 gagcgcctga tcctcaggcg tttcagggat tccgacctcg cggcgttcgt ggcctaccgc
       61 aacgaccccg aggttgcccg ctaccagtcc tgggattcga tcgatgagct ggaagccagg
      121 gctcttatca gggagatggc gtcggcgcga ccgggcgttc cgggcgattg gtttcagttc
      181 gcaatcgagt cgaaggagac gggggcgctg ctcggggact gtgccttgca ggtcgacggg
      241 caagaaccct acaaggcgga gctcgggttc acgctagcgc gggagcacca ggggaagggg
      301 ttcgcctcgg aagcggcctc ccgcctgctg gactacgcct tcgcctccct tgacctgcac
      361 agggtcttcg ccctcgcgga ctgcaggaac aggccctcct ggacgctgct tgacagggtg
      421 ggcatgcgtc gggaggggca cttcctggag aacgtctggt tcaagggtga gtggtgcgac
      481 gagtacctgt acgccgtcct gaaagacgag tggctcgaca ggccaacggg ctagccgtcc
      541 gacgtacccg cggctaggtc cgagaggcgc gccataacag ggcccgtcgc cgggtagagc
      601 gagcggtaga cctcgtagta ttccccgtag gctttcgagc gcccagcatc gggctcggtt
      661 agctcctccc gcagttccac cacggaggtc gcttcctcca cgtctgtata ggtaccagag
      721 gctacacccg ccaggagcgc ggcgccgtag gccggcccct cgtcggcgac ggtgcggcgg
      781 atgggggtgc cgtagatgtc ggcctggagc tggcgccaga gcccgctcct cgccccgcct
      841 cccgtggcgc ggacgtcttc tacgggcacg tcgagatctc tcattacctg cagggagtcg
      901 cggagggaga agaccacgcc ctccatcacg gccttcgtca tgtgggcgac gccgtggcgg
      961 gccgtcaggc cgaagaagga gcccctggct ttcgggtcga ggtgcggggt cctctcgccc
     1021 gataggtatg gcagaaagac tagaccctcg gagccgggcg gaacgttgga ggcctcctcg
     1081 accagctcgt cgaagtcgcc acctagcgtc tctctccacc aggagagcga cccaccggcc
     1141 gagagcgtca cgcccattag atggtaggcg cccggcacgg cgtgacagaa ggcgtgaagc
     1201 cgccccgaag gatccggctt gaactcctcc gtgtgggcga agaggactcc gctcgttccc
     1261 acggacgagt tgacgagccc gggttccacg atgccggtac caaccgcagc agcagcgttg
     1321 tcgccaccgc ccgcggcaac tggtatgcca gggagaaggc caagctcttc cgccacgctc
     1381 tcacggaggg caccggtgtt ctcggggcct tcgtagactt taggcatcca ctcgtgcggg
     1441 atctcgaggg cgtccaggat ctcaccggac cagtcgcggg ctcgaacgtc caacagcagc
     1501 gttcccgagg catcagaggc gtccgtggcg tgctcgcccg tgagcatgag cctcacgtag
     1561 tccttgggga gcaggacgcg ggagacccgg ccgtactctt ccggctcttc gtccctcaac
     1621 cagactatct tcggagcctg gaagccggtc agggcgggat ttccggcgat ggagatcagg
     1681 cgttccgcgc cgacggcctc agttatccgg tcgcactggg cctgggtgcg ctggtcgttc
     1741 cagagcaggg ccggccttac gacctcatca gaggagtcga ggaagacgga gccgtgcatc
     1801 tggcccgtga gaccgatgcc agccacctcc tcgctggcct cggaggcgac cctggcgagg
     1861 acctctttcg cgcccgtcca ccagtcttcg gggtcctgct cggtccagcc ggggcgtggg
     1921 gtgtggagcg ggaactcgga gcccgcctcc gcgacgatct tccccgagcc gtcgacggcg
     1981 acggccctgg cgccgccggt ccccacgtcg agcccgacga gtacgctcac gcctgactcc
     2041 tgccagcccg ggcttctcgg cggcgcctgc cgacctccac ctcgtagtct tcgtccatgc
     2101 caaggaccct gtacacctct ctctgcccgg cgagcgcgtc ggcccggtcc ctggccccgt
     2161 ccagggcgcc gcggtcgatc ttccgcgcca ggtcccagat gaactcccat tgcaggatgg
     2221 cgcgcctgac ggcggctatc tggtcttccg tgtaagggta gaggtcgaag cccacgatct
     2281 cgcccccctc cccgtagccc acgtcttgca gggtctgggc cagctcaagg gtctgcatga
     2341 agaagttggt gccgaccacg ttatcgtcgt cgaaggtgcc ccagccgtcg ttgccgtgct
     2401 ggtggccaag cttgtgctcg gaggcgagga gatcggcgta ctcggccagg ttctcgccgt
     2461 tcattattag gtgctgccag tccatgttta cctgcaggtt ggtggtgtcg accccgaggg
     2521 cgttcacctt ctgaatggta aacagcgtca tcccgatgtt ctgcatcagg atcttcatgg
     2581 ccggctcgga gtttttgtgc tccaggaaga ccttgacgtt cctctcgttg gcgtggtccg
     2641 tgagctcggc gaccccctct acgaaacgcc gccacgtctg ggcgtaaggg cgctggaacg
     2701 tgtagttgta accttcggcc cctggccaga tgatgaagtt cgccccgatc tgcgcgcaca
     2761 ggtcgatgcc gcgcctcaag gtgtctatcc cctggcgtct gaggccctca tcggggttga
     2821 cgaaggcgcc gagtgcgtag gtcgggtcgg ggtggagccc ggcggcgatt accggcaggt
     2881 ccatctcgtg ggaggcgagg atccgggtga cctcgtctac attgtcctcg tttatctcgc
     2941 cggggaaatg gtattccagg ccgtcgagca ggtcgttgag gccgccggcg acccgctccg
     3001 ttttgtccgt catgggctca tccgcgacct catggtggta gcctggcgga acgaaacgcg
     3061 tgacggccgg cccgaaagcc cagatgccaa cggagttctt gatcacaggg ttctccttcc
     3121 cctcggatag atacgagact gccctcccga ca
//
LOCUS       DBNGVD010000302         2661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2985357, whole genome shotgun sequence.
ACCESSION   DBNGVD010000302 DBNGVD010000000
VERSION     DBNGVD010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2985357"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16CM_08015"
     CDS             <1..307
                     /locus_tag="AC16CM_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR3139815.1"
                     /translation="EVELGQQLARTMASPAILDYVDFGEDEALIEARVPSEWLDKSLA
                     ELALSHEKGLTVVALKPQGGPGILPHGDTVLHSGDVLVVGGSKEALDRSPLIRPTDR"
     gene            320..1654
                     /gene="trkA"
                     /locus_tag="AC16CM_08020"
     CDS             320..1654
                     /gene="trkA"
                     /locus_tag="AC16CM_08020"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR3139816.1"
                     /translation="MRTVIIGAGEVGLSTARLLSHEGHSVVLVEKDEKLVEKATEELD
                     ALVIAGNGANPKLLREAGINRTDLLIAASSSDEVNIIACLAAKSQGVARTVARIHNAD
                     YYEPGDPFAEEMLGIDFIIHTEQMAAHEIKEALLVPGAINIDSFAGDTIEVAEVILVK
                     ESGAVDRPLKEVRLPDNSLIVGVVRGGEALVPRGDTVLRERDHVLLISGRRQISDAVK
                     VLATDTAPVREVTIYGGGRIGLRLALAVEKAGMSVRVIERDEARARYVASQLRRGFVL
                     HDEGISRDFMLQEGVHRTDAFVAVTADDRANLLAAMYARQLGARITIAGISRGEFAPL
                     ADALGVDVTISPRTLAAEAILRFVRKGEVIDVALLASGAEMIELRVPEDCRVAGRPLS
                     EVGFPEGALVGALLRDGDVIIPTGRDILRAGDDAVVFTIEDAVVEVENLFAR"
     gene            complement(1931..>2661)
                     /gene="rocF"
                     /locus_tag="AC16CM_08025"
     CDS             complement(1931..>2661)
                     /gene="rocF"
                     /locus_tag="AC16CM_08025"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HIR3139817.1"
                     /translation="EHVKHLDAVKRVCDEVAQRATSIVSDGLFPIFLGGDHSISIGTV
                     SGVAHSASGERTGLIWADAHADFNIPETSPSGNIHGMPLAALTGRGHPELVGVGGQSP
                     SVRTEDVVIIGLRSVDLEEKNLLREAGVKVYTMREVDVYGAGRIVRSAIRDLAHVDRV
                     HLSFDLDVVDPEVAPGVGTPIRGGLTYREAHLLMELLNEAGIITSLDMVEVNPILDVR
                     NGTATLAVELVESLMGRRIIDLPV"
BASE COUNT          476 a          840 c          903 g          442 t
ORIGIN      
        1 agaggttgag ctgggccagc agctcgccag aacgatggcc tcgccggcca tcctcgacta
       61 cgtcgacttc ggggaggacg aggccctgat cgaggcaagg gtgccttcgg agtggctcga
      121 caagtcgctc gccgagctgg cgctctcgca cgagaagggc ctcacggtgg tggcactcaa
      181 accccagggt ggtcccggca tcctgcccca cggtgacacc gtgttgcact cgggtgacgt
      241 tctcgtcgtc ggggggtcca aggaggccct ggacaggtca ccgctcatca ggccgaccga
      301 tcgttagggg gtacccggga tgaggacggt aataatagga gctggggagg tggggctgag
      361 cacagcccgt ctgctctcgc acgagggcca cagcgtcgtc ctggtggaga aagacgagaa
      421 gcttgtggag aaggctaccg aggagctaga cgcgctcgtc atcgccggga acggcgccaa
      481 cccgaagctg ctcagggagg ccggcatcaa caggaccgac ctgctaatag ctgcgagcag
      541 ctccgatgag gtcaacatca tcgcgtgcct cgccgccaag tctcagggcg tggcacgcac
      601 cgtggcgcgc atccacaacg ccgactacta cgagccagga gacccgttcg ccgaggagat
      661 gctcggaata gacttcataa tccataccga gcagatggcg gcccacgaga tcaaggaggc
      721 cctcctcgta cccggcgcca tcaacataga cagcttcgcc ggggacacca tcgaggtggc
      781 agaggtgatc ctggtcaagg aatcgggcgc cgtcgaccgc cccctcaagg aggtaaggct
      841 ccccgacaac agcctcatcg tcggcgtcgt caggggcggc gaggcgctcg ttccaagggg
      901 cgataccgtg ctccgagaga gggaccatgt gctcctgatc agcggccggc gccagatctc
      961 cgacgccgtc aaagtgctcg ctaccgacac ggcaccggtc cgggaggtga ccatctacgg
     1021 gggcggccga atcgggctga ggctggcgct cgcggtggag aaggctggca tgtcggtcag
     1081 ggttatcgag cgggatgagg cccgcgcccg ctacgtggct tcacaactaa ggagaggctt
     1141 cgtgctgcac gacgagggga tctcgcgtga cttcatgctg caggagggcg tgcacaggac
     1201 agacgccttc gtggccgtga ccgccgacga ccgggccaac ctgctcgcgg cgatgtacgc
     1261 ccgccagctc ggggcccgca taaccatcgc cggcatcagc cggggggagt tcgcgccgct
     1321 agccgacgcc ctcggcgtgg atgtgaccat ctcgcccagg acgctcgccg cagaggccat
     1381 cctgcgcttc gttcgcaagg gcgaggtcat cgacgtggcg ttgctggcga gcggggccga
     1441 gatgatcgaa ctgcgcgtcc ccgaagattg cagggtggca ggccgcccac tctccgaggt
     1501 gggcttcccc gagggcgccc tcgtgggggc cctgctgcgc gacggcgacg tgatcatacc
     1561 cacgggcagg gatatcctca gggccggcga cgacgcggtc gtgttcacca tcgaagacgc
     1621 cgtggtagag gtcgaaaatc tcttcgccag gtgaagcacg ctccggcttc gcctccgctt
     1681 gtcagcacac tacggccttc ggcctccgtt tgtcagcacg ctccctgcgg tcacttgtca
     1741 gcaacgcttc gccgggcttc gctcggctcg ctttcagcca gatcccgctg cgtccgcacc
     1801 taccgcacga cagtaacggt gcccggaaaa gccctatgtc gaagccgccg ttacggaccg
     1861 agacgaccag ccgaaggcga ggcgaagccg aagcgttgcc gacaagcgag ggcgagagcc
     1921 ggagcgtgct ctacactggc aggtctatga ttcttcgtcc catcaggctc tctacgagtt
     1981 ctactgccag ggtggccgtg ccgtttcgta cgtccagaat ggggttgacc tctaccatgt
     2041 ccagggaggt tatgatgccg gcctcgttga ggagctccat gaggaggtgg gcttcccggt
     2101 aggtcaggcc gccgcgtatc ggggtgccaa cgccgggtgc tacctcgggg tcaaccacgt
     2161 ccaggtcgaa cgagaggtgg acgcggtcta cgtgggcgag gtccctgatg gcgcttcgaa
     2221 cgatgcgtcc ggcgccgtag acgtccacct ccctcatggt gtagaccttg actcctgctt
     2281 cgcgcagcag gttcttctct tccaggtcca cggagcggag gccgatgatc accacgtctt
     2341 cggttctgac gctcgggctc tggcccccca cgccgaccag ctcagggtgg cccctacccg
     2401 ttagcgcggc gaggggcatg ccgtggatgt tgccggaagg ggaggtctct gggatgttga
     2461 aatccgcgtg ggcgtcggcc cagatcaggc cagtccgctc tcccgaggcg gagtgagcga
     2521 cccctgatac tgtcccgatg gagatggagt ggtccccacc cagaaagatg ggaaagagcc
     2581 cgtcggagac gatggaagtg gcccgctggg cgacctcatc gcagacgcgc ttgacggcgt
     2641 cgagatgctt gacgtgttcg c
//
LOCUS       DBNGVD010000303         3383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3349048, whole genome shotgun sequence.
ACCESSION   DBNGVD010000303 DBNGVD010000000
VERSION     DBNGVD010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3349048"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="AC16CM_08030"
     CDS             <1..1206
                     /locus_tag="AC16CM_08030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3139818.1"
                     /translation="LCTHFIQPSAVPTMERLGLLPLIEAAGAVPNEIDVWTRWGWVRT
                     PEGASHGYNIRRETLDPMLRRLASETPGVDLMPGQMAFRLLREDGDENGRISGVAIRD
                     RAGETQEISARLVVAADGRDSKMARLASVPSKSRPNNRFVYFAYYRNLHTVSGKRSQM
                     WLLEPDTAYTFPNDDGVTLLTCLPARQKLPAFKKDLEGSFERFFEGLPEGPRLAGAER
                     VSKVMGATNLVNRSRPASVDGLAFVGDAALWSDPLWGVGCGWAFQSAEWLADEIASAL
                     ANGGDLHEALARYRDKHRKKLSGHQRIISDFSAANSYTPVERLMFSAAARDEQSARHF
                     HAFGSRLVGVREFLAPRAVARALFVNVRHAAGRLNGRGTERSEKALAGRVAYVNTIGT
                     SKPGEEKAR"
     gene            1203..2045
                     /locus_tag="AC16CM_08035"
     CDS             1203..2045
                     /locus_tag="AC16CM_08035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3139819.1"
                     /translation="MTTDTPGTKSPAYLMGRSEAETRRLIAQHQLYARFTRRLLEDAG
                     IAGGMKVLDVGSGAGDVALLAAELVGPTGMVLGVDQDPVVLETASARAEASGLTNVSF
                     HVGNLPQDVPGDDFDAVVGRLVLLYVPDPAIALRGLVERLEPGGIVAFGEFNFLPDSM
                     VTHPPTPSGESLWGWMQAVVRGIDLDPTTGYHLRNTFLEAGLPEPEMNSCAPVGGGPD
                     FPGYDYGAESLRSMLPLIFKLGIATEEEVEIDTLARRLRDDVVASGGVIKTPELVGAW
                     TRKN"
     gene            complement(2136..2690)
                     /locus_tag="AC16CM_08040"
     CDS             complement(2136..2690)
                     /locus_tag="AC16CM_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139820.1"
                     /translation="MTDSIRTFDRMGETAQTMVRTSQESARIFTDYTVKAQEANTQYA
                     RRAVETWIDGLRKQTELSQEVAQQLFGKAEEQADVYRSFFGQWGNVPAMRFPFAGAAN
                     DFMFPFQRQGMRLVETGVRNTEEVIDTASFPIANYDEKNVDEISARLDSLGEDQIRRL
                     RSYEKAHKNRQTLIERFDTKLRAS"
     gene            complement(2803..>3383)
                     /locus_tag="AC16CM_08045"
     CDS             complement(2803..>3383)
                     /locus_tag="AC16CM_08045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3139821.1"
                     /translation="FGLPFQESIKEDGWGGREQEDIRCGIEALIDAGISRPGRVGVTG
                     TSYGGYSAWWAITHFSPRVVAAAAPICGMTDLVVDYNATRPDLRPYSEEMMGGTPDSV
                     PKVYRERSPIEAVHNIHGELLIVQGLKDPNVTPDNVEAVTVVLHRQNIPYELLTFEDE
                     GHGIARPENLHILYPHIADFFLRAFDKTPTPE"
BASE COUNT          588 a         1078 c         1081 g          636 t
ORIGIN      
        1 ctatgcaccc acttcatcca gccgagcgcc gtgccgacca tggagaggct aggcctcctt
       61 cccttgatcg aagcggccgg ggcggtgccg aacgagatag acgtgtggac ccgatggggt
      121 tgggtccgca ctcccgaggg cgccagccac ggctacaaca tccgccgcga gaccctggat
      181 cccatgctcc ggcgcctcgc ctcggagacg ccaggtgtgg atctcatgcc cggccagatg
      241 gccttcaggc tgttgaggga ggatggtgat gagaacggcc ggatctccgg cgtcgccatc
      301 agggaccggg ccggagagac ccaggagatc tccgcgcggc tcgtggtggc cgccgacggc
      361 cgcgactcga agatggcgcg gctggctagc gtgccttcga agtctagacc caacaaccgc
      421 tttgtctact tcgcctacta ccgcaacctg catacggtct cgggcaagag atcccagatg
      481 tggctgctcg aacccgacac ggcctacacc ttccccaacg acgacggcgt gacgctgctg
      541 acctgcctgc ccgccaggca gaaattgccc gccttcaaga aggatctgga aggtagcttc
      601 gagcgcttct tcgagggcct gccggaaggg ccacgattgg ccggcgcgga gcgcgtctcg
      661 aaggtaatgg gtgcaaccaa cctcgtcaac cgctcgcgcc ccgcctccgt ggatggcctc
      721 gccttcgtgg gcgacgcggc cctctggtcg gacccccttt ggggcgtcgg ctgtgggtgg
      781 gccttccaat cggcggaatg gctcgcagac gagatcgcgt ccgctctggc gaacggcggc
      841 gatctccatg aggcgctcgc ccgctaccgc gacaagcaca ggaagaagct ctccgggcac
      901 cagcgcatca tctccgactt ctccgcagca aattcctaca ctcccgtaga gaggctgatg
      961 ttctctgcag cggcaaggga cgaacagtct gccagacact tccacgcctt cggctcccgg
     1021 ctagtcgggg tgcgcgagtt ccttgcaccg agggcggtcg cccgtgccct gttcgtcaac
     1081 gtgcgacacg ccgcgggacg cctgaacggt cgtggaacag agcgttccga aaaagcactc
     1141 gccggtcgtg tcgcgtacgt caacactatc ggtacgagca aaccgggaga ggagaaagcg
     1201 agatgaccac cgacacccca gggacaaagt cacccgccta cctcatgggc cgaagcgagg
     1261 ccgagacgcg gcgcttgata gcgcaacacc aactctatgc ccgcttcacg cgacgcctgc
     1321 tcgaggatgc cggcatcgca gggggcatga aggtcctcga cgtgggcagc ggggccgggg
     1381 atgtggcgct gcttgccgcc gagctcgtcg gcccgacggg gatggtgctc ggggtagacc
     1441 aggaccccgt ggtcctcgag accgcctctg cccgcgccga agcgtccggc ctcaccaacg
     1501 tctccttcca cgtcggaaac ctcccccagg acgttcccgg cgatgacttc gacgccgtgg
     1561 tggggaggct tgttctgctc tacgttccgg atccagccat agcactcagg gggctggtcg
     1621 agcgactcga gccagggggt atcgtggcat tcggggagtt caactttctt cctgactcca
     1681 tggttaccca tcctcccacc ccgtctgggg agagcctgtg gggctggatg caggccgtgg
     1741 ttcgcggcat cgacctcgac cccacgacgg gctaccatct cagaaacacc tttctggagg
     1801 cggggctacc cgagccagag atgaacagct gcgcaccggt cggcggggga ccagactttc
     1861 ccggctacga ctacggcgcc gagtccctgc ggagcatgct gcccctcatc ttcaagctcg
     1921 gcatcgccac ggaggaagag gtcgagatag ataccctggc ccggaggctt cgcgacgacg
     1981 tcgtggcctc gggcggcgtc attaaaactc cagagctggt aggagcctgg acgcgcaaga
     2041 actgaacaac gcccgattgg cgatgcccag aagacacgaa agaagggccg cggcttgaga
     2101 ccacggccct tcttttcagg attgtctgga ctctgttacg aagccctcag cttggtgtcg
     2161 aagcgctcga tcagggtctg gcggttcttg tgggccttct cgtagctcct gaggcgtcgt
     2221 atctggtctt cgccgaggct atcgagccgc gcggagatct catcgacgtt cttctcgtcg
     2281 tagttggcga tggggaacga ggcggtgtct atgacctcct cggtgttcct gacgccggtc
     2341 tcgacgagcc tcatgccctg cctctgaaag gggaacatga agtcgtttgc cgcgcccgca
     2401 aacgggaaac gcatcgcggg aacgttgccc cactggccga agaacgaacg gtacacgtcg
     2461 gcctgctcct cagccttgcc gaagagctgc tgcgcgacct cctgggacaa ctcggtctgc
     2521 ttgcgcagcc cgtcgatcca ggtctcgacc gcccgccggg cgtactgcgt gttggcttcc
     2581 tgcgccttga ccgtgtagtc agtaaagatc ctggccgact cctgcgaagt ccggaccatg
     2641 gtctgcgcgg tctcgcccat cctgtcaaac gtcctgatgc tgtcggtcac tctgattcct
     2701 ccatgtcgaa gctttctact gccttacagc acttatgtgt accccgcaca tacaggcact
     2761 aaacatcctg acacggaatt ttctggcatc gcatttcagg cgttattccg gtgtgggggt
     2821 cttgtcgaaa gccctcagga agaaatccgc gatgtggggg tagaggatat ggaggttttc
     2881 cggtctggct atgccgtggc cctcgtcttc gaaggtgagg agttcgtagg ggatgttctg
     2941 gcggtgcagt actaccgtca cggcttcgac gttatccggg gtgacgttcg ggtccttgag
     3001 gccctgaacg atcagtagtt cgccgtggat attatggacg gcctctatgg gggatctttc
     3061 gcggtagacc ttggggacgc tgtcgggggt gccgcccatc atctcttcgc tgtaggggcg
     3121 caggtcgggg cgggtagcgt tgtagtcgac cacgaggtcg gtcatgccgc agatcggggc
     3181 cgcggcggcg accacccggg ggctgaagtg cgtgatcgcc caccacgccg agtagccgcc
     3241 gtatgaggtg cccgtcacgc cgacgcggcc gggacgtgag atgccggcgt ctataagggc
     3301 ctcgatcccg cacctgatgt cttcctgctc gcggccgccc cagccgtcct ccttgatgga
     3361 ttcctggaag ggtagtccga acc
//
LOCUS       DBNGVD010000304         4826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4441961, whole genome shotgun sequence.
ACCESSION   DBNGVD010000304 DBNGVD010000000
VERSION     DBNGVD010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4441961"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="prfA"
                     /locus_tag="AC16CM_08050"
     CDS             <1..1068
                     /gene="prfA"
                     /locus_tag="AC16CM_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3139822.1"
                     /translation="EETLGRYDALTKELSDPDIFNDQRHYAEVAKEHARMRRGAEMSR
                     ELLDAVEAERDARELVPQAENAEEREFFQAEARAAEEQIVDLSERIRTELIDRDPNDD
                     KDVIIEIRGGAGGDEAALFAGELHDMYTRYADRLGFKHRTLSSSPAEVGGYKEVAVEI
                     SGDRAYSVFKHEGGTHRVQRVPKTESQGRIHTSTATVAVLPEAEDVEVEIHQGDLEVD
                     VYRSSGPGGQSVNTTDSAVRITHKPTGLVVTCQNEKSQLQNKEQAMRILKSRLLEREM
                     RERAEQEGALRQAQVGTGDRSAKVRTYNFPQGRITDHRVGVTSHNLEATLGGDLEEFT
                     QALAAKERADKLAAEAAGTPG"
     gene            1037..1942
                     /gene="prmC"
                     /locus_tag="AC16CM_08055"
     CDS             1037..1942
                     /gene="prmC"
                     /locus_tag="AC16CM_08055"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3139823.1"
                     /translation="MPPKQREPPASTVRAGGAGAREAVRQAAGTLERAGVPEPAASAE
                     VLLSELLGIGRAEIPLYQKPLTDEQASRYGAWISRRLEREPVQRILGYAYFRNLVLDL
                     NEDTLIPRPDTESVVDLALERIDLRGYPCTVLDVGTGTGAIAISIAQERPRCEVHATE
                     VSEGALDAARANADRNDTDVRFHHADVVSGLGFLHGGIDLLVSNPPYVDETSASRRLA
                     PEVRRWDPAVALYSGKDELSFFRRIFAETPTLLKGGADVVLEIGDGQAERVLDLGKSS
                     GFSPLGVRNDLAGTPRAVLLRWEGS"
     gene            1939..2505
                     /locus_tag="AC16CM_08060"
     CDS             1939..2505
                     /locus_tag="AC16CM_08060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3139824.1"
                     /translation="MKELSPREAVEGLRSGEVALVPTETVFGLVADEPGLSRIREIKG
                     RDDKKPIALLCRSAPEAFGLASNVPPLAQKLADLYWPGPLTLVLDLPTGETIGLRVPA
                     GRTVRELLEAYGGPLYATSANLSGEPARSTPDQVDPQVLAAVDIVVVGEGGSGEASAV
                     VGLSGEKVRLLRPTDELTPEKLAQLAGE"
     gene            complement(2517..3452)
                     /locus_tag="AC16CM_08065"
     CDS             complement(2517..3452)
                     /locus_tag="AC16CM_08065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HIR3139825.1"
                     /translation="MNVESGESGARIEAIRRFNRFFTRTIGVLRGGLLHTPYSLTEAR
                     VLFEVANREEATASDLSRELGLDPGYLSRTLSGLERGGLICRDTSEADGRRRVLSLTA
                     DGVDAFELLDTRSREEIAELLDGLSEEEQRRLLGAMRSIEGLLGKGLKFSGPFFVRSH
                     EPGDMGWVVQRHGELYLQEYGWDERFEALVAHIVADFIDGYDPSGERCWIAEMEGERV
                     GCVFCVRESKTVAKLRLLLVEPEARGLGLGGHLVDECIRFTRSHGYQRLTLWTNSVLD
                     AARHIYESRGFRLVDQEVHESFGRELVGQNWELEL"
     gene            3625..4650
                     /locus_tag="AC16CM_08070"
     CDS             3625..4650
                     /locus_tag="AC16CM_08070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3139826.1"
                     /translation="MPTRPKLYLRSYAVPDDMEAHAAAVEEDGWDGMFFMDSQNLSMD
                     VFGSLYLAASATSRLELGTAVTNLVTRHPAVTASSFATLHHVSGGRAHIGVGRGDTAL
                     ELVGIKPPSAARFGELLGELQGYLRGDAIDVNGFQSCVSWLPFQGESKVPVDVFGSGP
                     HVLDVGARHGDKLTAAVGAEPERIAWAVETAREARRDAGLDPETLDVGAFVVVGAGTD
                     SRALDELVRGNASISAHFQRDVTSSLSHTDASVVEKVTSHYDNYHHGLEHASQAESIP
                     ADFLRRFCVIGPPEECAERLCGLIGQGLSHLVLVGGSRDIDTTVRERSDHLVASEVLP
                     AIRAAFA"
BASE COUNT          897 a         1519 c         1603 g          807 t
ORIGIN      
        1 gaggagacgc tcggccgcta cgacgcgtta acgaaagagc tctctgatcc cgacatcttc
       61 aacgaccagc ggcattacgc cgaggtcgca aaggagcatg cgcgcatgcg tcgcggggcc
      121 gagatgagca gggagttgct cgacgccgtg gaggcggagc gcgacgcccg cgagctcgtt
      181 ccccaggccg agaacgcgga ggagcgcgag ttcttccagg ccgaggcgcg cgcggccgag
      241 gagcagatcg ttgacctctc ggagcgaatc agaacggagc tcatagaccg cgacccgaac
      301 gacgacaagg atgtcataat cgagatccgt ggcggcgcgg gcggcgacga ggcggccctt
      361 ttcgccggcg agctgcacga tatgtacacc cgctacgcag acaggctcgg tttcaagcac
      421 aggacgctct cctccagtcc tgccgaggtg ggcggctaca aggaggtagc cgtggagatc
      481 agcggcgaca gggcctactc ggtcttcaag cacgagggtg ggacgcatcg cgtccagcgg
      541 gtgccgaaga cggagagcca gggccgcatc cacacctcga cggctaccgt ggccgtgctc
      601 cccgaggcag aggacgtgga ggtggagatc caccagggcg acctggaggt ggatgtctac
      661 cgctccagcg gccccggtgg gcagagcgtg aacaccacgg actcggcggt caggatcacg
      721 cacaagccga cgggcctcgt ggtgacctgc cagaacgaga agagccagct ccagaacaag
      781 gagcaggcca tgaggatcct gaaatcccgt cttctggaac gtgagatgcg tgagagggca
      841 gagcaagaag gcgcgctccg acaggcccag gttggcacgg gagaccgctc ggccaaggtc
      901 cgtacctaca atttcccaca ggggcgcatt acggaccacc gcgtcggcgt aacctcccac
      961 aacctcgagg caaccctcgg cggcgacctc gaggagttca cgcaggccct cgccgccaag
     1021 gagagggctg acaagcttgc cgccgaagca gcgggaaccc ccggctagca cggtgcgcgc
     1081 aggcggggca ggcgcccgtg aggccgtacg tcaagctgca gggacgctgg agcgggccgg
     1141 cgttcctgag ccagccgcct ccgccgaggt cctcctctcc gagcttctcg gcatagggcg
     1201 ggccgagatc cccctttatc agaaaccgct gacggacgag caagcctcga ggtacggggc
     1261 ctggatctcc cgacgactcg agcgcgagcc ggtgcagcgc attctgggct acgcatactt
     1321 caggaacctg gtcctcgacc tgaacgagga cacgctcata ccgcgcccgg ataccgaaag
     1381 cgtggtcgat ctcgcgctgg aacgaataga cctgcgcggc tatccctgca cggtcctcga
     1441 cgtcggaacc ggcacagggg ccatcgcgat ctccatagcc caggaacgtc cccgctgcga
     1501 agtccacgcc accgaggtat ccgagggcgc cctcgatgcc gcgcgggcca acgccgaccg
     1561 taacgacacc gacgtgcgtt tccaccacgc ggacgtggtc tcggggctcg ggttcttgca
     1621 tggggggatc gaccttctcg tctcgaaccc tccttacgtg gatgagacca gcgcctcccg
     1681 ccggctcgcc ccggaggtga gaaggtggga ccccgcggtc gccctctaca gcggcaagga
     1741 tgagctatcg ttcttccggc gcatattcgc ggagacgccg acgctgctga agggtggggc
     1801 cgatgtcgtg ctcgagatcg gtgacggtca ggccgagagg gtgctggatc tcgggaagtc
     1861 gagcggcttc tcgcccctgg gagtgcgcaa cgacctggcg ggcacaccaa gggccgtgct
     1921 gctccggtgg gagggttctt gaaagagttg tcaccacggg aagcggtcga gggcttgaga
     1981 tccggcgagg tcgccctcgt acccacggag acggtcttcg gcctcgtcgc cgatgagcca
     2041 ggcttgtcgc gcatcaggga gatcaaaggc cgcgacgata agaaaccaat cgcgcttctc
     2101 tgccgctcgg cgccggaggc cttcgggttg gcgtcgaatg tacccccgct cgcgcaaaag
     2161 ctcgccgacc tctactggcc gggtcctctg acgctggtcc tggatctacc cacaggagag
     2221 accatcgggc tgagggtgcc cgccggccgg accgtgcggg agcttctgga ggcctacggc
     2281 ggccctctct acgccacgag tgcgaacctc tccggcgaac ccgcgaggtc gacacccgat
     2341 caggtcgacc cccaggtgct cgcggccgtg gacatcgtag tggtaggaga agggggtagc
     2401 ggcgaggcgt cggccgtcgt cggtctttca ggggaaaagg tacgactcct tcgtcccacc
     2461 gacgaactga cgccggagaa gctggcccag ctcgccggcg aatgaggaga cgggtctcat
     2521 aactcgagtt cccagttctg cccgacgagc tctcgaccga agctttcgtg cacctcttga
     2581 tctacgagcc tgaacccccg actttcgtag atgtgccggg cggcgtccag cacgctgtta
     2641 gtccagagcg tgagcctctg atagccgtgg ctcctggtga accggatgca ctcgtcgacg
     2701 agatgtcctc ccagcccgag accgcgcgcc tccggctcga ccagtagcaa cctcaacttt
     2761 gccaccgtct tgctctctct tacgcagaag acgcacccga ccctctcgcc ctccatctcg
     2821 gcgatccaac atcgctcccc ggatgggtcg tagccgtcga tgaagtcggc cacgatgtgc
     2881 gccaccaacg cctcgaaacg ctcatcccaa ccgtactcct ggaggtacag ttccccgtgt
     2941 cgctgaacga cccagcccat gtcgccgggc tcgtgggacc gtacgaagaa aggcccggag
     3001 aacttgagcc ccttgccgag aagaccctcg atgctcctca tggccccgag cagccgtcgc
     3061 tgctcttctt cggagagccc gtccagcaac tcggctatct cttcgcgcga acgggtatcg
     3121 agcaattcga aggcgtccac gccatcggcc gtgagggaga gaacgcggcg ccggccatcg
     3181 gcctcggagg tgtccctgca gatgaggccc ccgcgctcca gaccagacaa cgtccggctc
     3241 agataaccgg gatccaaccc cagctcgcgc gagagatccg aggcggtcgc ctcctcacgg
     3301 ttggccacct cgaagagcac ccgggcctcg gtcaacgagt agggcgtgtg gagcaggcca
     3361 ccgcgcagaa ccccgatggt tcgcgtgaag aagcggttga agcgcctgat ggcctctatc
     3421 ctggcccctg attccccact ctccacgttc acctcctgga gcaacacttt atggtgggat
     3481 atttaattgc ttttgtcaac taaatgcaag gatgcggttg gttcttgtcg ggtagtgagg
     3541 gtggggagta tccgggcagc ggtgtggtct ggtggtgttt aatgtggagg cgtgaggcgc
     3601 tagtgtgatc ggagaggagc gctgttgccg acgagaccca aactctatct gagatcctac
     3661 gccgttcctg acgacatgga ggcgcatgct gctgcggtgg aggaagacgg ctgggatggc
     3721 atgttcttca tggattcgca gaacctgagc atggatgtgt ttggatctct ttaccttgcg
     3781 gcttcggcca cctcgcggct ggaactgggt acggccgtga ccaacctcgt gacgcgccat
     3841 ccggccgtca cggcctcgtc gttcgcgacc ctgcaccatg tctctggagg gcgggcgcac
     3901 atcggggtcg gcaggggcga cacggcgctc gagctggtgg ggatcaaacc accctcggcg
     3961 gcgcggttcg gggagttgct cggggagctt cagggttatc tgcgcggcga tgcgatcgac
     4021 gtcaacggct ttcagagctg cgtgagctgg ctgccatttc agggggaatc caaggtgccg
     4081 gtagacgttt ttggatctgg gccgcacgtt ctcgacgtcg gagccaggca cggtgacaaa
     4141 ctaacagcgg ccgttggcgc cgagccggag aggattgcgt gggcggtcga aacggcccgt
     4201 gaggcgagga gggacgcggg cctggatccg gagaccctgg atgtgggtgc cttcgtcgtg
     4261 gtcggtgcgg gtacggacag ccgggccctc gacgagctcg tcagggggaa cgcgagcatc
     4321 tcggcacact tccaacgcga cgtaacgtcc tcgctgtcgc acaccgatgc cagcgtggtc
     4381 gagaaggtta cgagccatta cgacaactac catcacgggc tcgagcatgc ctcccaggcc
     4441 gagtccatcc ccgccgattt cctcaggcgt ttctgcgtca taggcccgcc cgaggagtgt
     4501 gccgagaggc tgtgcgggct gatcggtcag ggtctatctc acctggtcct ggttggaggc
     4561 tctcgcgaca tcgataccac ggtgcgcgag cgctccgacc atctggtcgc aagcgaagtg
     4621 ctgccagcca tacgcgccgc gttcgcgtag ggaggccacc ggagggtcat gggcggcgaa
     4681 cggttttcgt acaggatcac gaagaacggc aagcttttcg tgtactggca tggcacggat
     4741 ggaaaacgtg agatcgtctt gaagggcgct aagtccgagc ggcttatcac acagctaccg
     4801 gcgatggatc acgaagcgca gcaact
//
LOCUS       DBNGVD010000305         5614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1346602, whole genome shotgun sequence.
ACCESSION   DBNGVD010000305 DBNGVD010000000
VERSION     DBNGVD010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1346602"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1089)
                     /locus_tag="AC16CM_08075"
     CDS             complement(58..1089)
                     /locus_tag="AC16CM_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019505404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139827.1"
                     /translation="MGEKRRAGKMVVVTGGNAGLGYECARAIAASGQRWRVVLAVRSM
                     EKGERAAVRIAEETANPEVEAMALDLASLASVRSFAGTLARRDDLPPLRALVCNAGVQ
                     VVSGTSYTEDGFEETFGVNHLGHFLLVNLLLGRLEAPARIVSVSSGTHDPERKTGMPA
                     PHYRGAEAMAHPEKYPGPVEESEGPGKIGRRRYTTAKLCNVMFAYELDRRLREEGIST
                     PGAPIDTNAFDPGAVPGTGLARDWGPLARLTWDGGVRKLVPILRRMGVSFNTPEASGR
                     SLARLVTDPSLRGVSGAYFEIDERARSSEESYDREKARELWKTSAALVDLHSAETPLR
                     ATAAASGGS"
     gene            1186..1782
                     /locus_tag="AC16CM_08080"
     CDS             1186..1782
                     /locus_tag="AC16CM_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3139828.1"
                     /translation="MTRGASSTARGGETRSALLGVAAGLLEEGGMGAVTLRAVGDRAG
                     VSRQAPYNHFSDKAALLGALAARYLGRLDEEMTVAAGEAGEDAFEALGAMTVAYVRFA
                     LESPSRYRLIGREMSGLRLEVHEAARAVHERFVRTVGACQEAGELPGGDPEELAAIVF
                     ATTHGAVELALSGHTEEEKGLGDPARLVRRLLDRLRRG"
     gene            complement(2012..2482)
                     /locus_tag="AC16CM_08085"
     CDS             complement(2012..2482)
                     /locus_tag="AC16CM_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139829.1"
                     /translation="MSAEANKPIKDMTHEEALEALPRLRTEAAVANARVHEVMDRVAQ
                     TEDIPTHVDVHGDHLVISVRGKELAMTFLSDLRVPLEHVERAEADPNIEHTLWRGWRV
                     PGVHLPGVRFYDVHGHRDKTIVIYLKDETYDRLIVEVSDPDAVIAKINEALETG"
     gene            2632..3516
                     /locus_tag="AC16CM_08090"
     CDS             2632..3516
                     /locus_tag="AC16CM_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3139830.1"
                     /translation="MSEARNRAAVATEISLVFAAIFWGLNFAATKYATATIPPLAFVT
                     LRFAAGGLLLLLVLRLLEPKSKLRRGDLLPMAALGCLGVATAQTGFTYGVSLTTAGST
                     GLIFATAPVWGLLLGAVLGLERPTWKGVAGVGLSIVGVALVVLDGLLAGHASLVGDLL
                     VLVAALCVGAYAVFSMPLLEHHTPLAVATYPVLFGIPLLLILSSPQLLSLEWGDVGAG
                     PWAAVAYAGVFATAFSFSAWQGGISRIGANRVLVYQYLITAVGVTSGIVFFGEALSVN
                     KVVGGAVILVGVYLARRQ"
     gene            complement(3544..>5614)
                     /locus_tag="AC16CM_08095"
     CDS             complement(3544..>5614)
                     /locus_tag="AC16CM_08095"
                     /EC_number="3.2.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF012906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004567 - beta-mannosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005537 - D-mannose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HIR3139831.1"
                     /translation="HPDQWGRNPERVSMRKAQFGFGWDWGPRLPTVGIWRPVELRRER
                     RARIRGVHFHTLEIAPSGERVAVAVRVEADRFAAEGAIEASISLTAPGGEPVSGATLT
                     LEGEGADLTGTAYVSVERPCLWWTHDLGRPSLYGLEVALRQDGDVLDTHASKVGIRTL
                     ELDQSPDPDEPGTRFFRFVLNGVPIFARGADWIPADSFVGAIPRERYDNLIAAARDAN
                     MNMLRVWGGGIYEHDAFYELCDSLGILVWQDFMFACAVYPEEPASFVAEVEEEARYQV
                     RRLRGHACLALFCGNNENQWIHDRTYWDRDDTTVPGTLYYHEVLPRVVAELDGRTPYW
                     PGSPFGGDDHNDRRQGNVHNWEVWHGNFPRHFGQQPGRNTAPESVSYLRYAEDSGRFI
                     SEFGMHASPVRETLRRAIPEDQLYHHSPSMDHHNKDDPKHKGDNLMSTVTGLPRDLDE
                     YVDFSMISQAEGLKFALEHFRRRKPHCSGTLIWQLNDCWPVLSWSVLDHNGFGKAGYF
                     YARRAYSPLLASFKMLPDGSVELWLTNDTLSLMRDRVTVHLATFGGEKIWEENHSVEV
                     SSNASRPVARWTAEQASGAPDRYLSVTSSGDHFPSNRHFFAPIKDLEREPVEPHVEIV
                     PKGDHQLSVRLEAPSYAYFVNLEVSHEATHFSDNYIDLVPGEKREIQVTNERCRLSRE
                     MVRVRWR"
BASE COUNT          860 a         1789 c         1884 g         1081 t
ORIGIN      
        1 ccgtttgtcg caggttctaa tcctatcggt cccgctcgac gaataggaca agctcaccta
       61 ggagcctccg gacgctgcgg ccgtggcgcg tagcggtgtc tccgccgagt ggagatcaac
      121 cagcgcggcg ctcgtcttcc agagctccct tgccttctcc ctgtcgtagg attcctccga
      181 agaacgtgcc cgttcgtcta tctcgaagta cgcgcccgag acgccccgca gtgacgggtc
      241 tgtcacgagg cgggccagag accggccgga tgcctcgggc gtgttgaagg agacgcccat
      301 acgacgcaag atggggacga gtttcctcac ccctccgtcc caggtgaggc gtgccagcgg
      361 cccccaatca cgcgccaggc cggtgcctgg tacggccccg gggtcgaacg cgttcgtatc
      421 gatcggggcc ccaggggtgc ttatcccctc ctctcgcagg cgccggtcga gctcgtaggc
      481 gaacatgacg ttgcagagtt tggcggtcgt gtagcgcctc ctgccgatct tgcccggacc
      541 ctcgctctct tctacgggac cggggtactt ctcagggtgc gccatcgcct cggcgccgcg
      601 atagtgcggg gccggcatgc cggtcttgcg ctcggggtcg tgggtgccgc tggagacgga
      661 gacgatcctc gccggggcct cgagccgccc gagcagcaag ttcacgagca ggaagtggcc
      721 gaggtggttg accccgaacg tctcctcgaa gccgtcctcc gtgtaacttg tgccgctcac
      781 cacctgcacc ccggcgttgc acacgagggc ccggagcggt ggcagatcgt cgcgccgggc
      841 gagcgtcccg gcgaatgacc gtacggaggc gagcgacgcg agatccaggg ccatcgcctc
      901 gacctccggg ttcgcggtct cctccgcgat ccgcaccgcc gcccgctcgc ccttctccat
      961 gctgcgaaca gcaaggacga cacgccaccg ttggcccgag gcggcaatcg ccctcgcaca
     1021 ctcgtagccg agccccgcgt tgccgcccgt tacgactacc atcttcccag ccctccgttt
     1081 ttcccccatc tctttctcct tcgctgctcg acctcgcgta ttgacaccgt caacattacc
     1141 cgttcgtgtt gacgtcgtca acctggtacg ggatactcgt tcgtgatgac caggggagcc
     1201 tcatctacgg cgcggggtgg cgagactcgc tcggccttac tgggggttgc ggccggtctg
     1261 ctcgaggagg gggggatggg cgccgtgacc ttgcgcgctg tgggggaccg ggcgggggtc
     1321 tcgaggcagg caccttacaa ccacttctcc gacaaggcgg cgctcctggg tgcgcttgct
     1381 gcgcgctacc tcgggcgtct ggacgaggag atgaccgtgg ccgccgggga ggctggtgag
     1441 gatgcgttcg aggctctcgg agcgatgacg gtggcctacg tgcgcttcgc gctggagagc
     1501 ccgtccaggt accgcctgat cggccgggag atgagcggcc ttcgtctgga ggttcacgag
     1561 gcggcccggg ccgtgcacga gcggttcgtg cggacggtcg gggcgtgcca ggaggctggc
     1621 gagctgcccg gtggcgaccc tgaagagctg gcggcgatcg tgtttgcgac cacccatggg
     1681 gcggtggagc tagcgctctc cggtcacacc gaagaggaga agggactggg cgatcccgcc
     1741 agactcgtgc gccgcctact cgaccggctg cgtcgcggtt gacgctcgtt gctgttcagg
     1801 ggtaagtagg gaaaagacga gaggcagcgt gtcgcaaggg gccagggtac tcgcggagga
     1861 gttccagctg aagatacgct ctctgcaggc ccctctccgc gcagacgcgc ggagccttca
     1921 cgggcaagag aggctctatg gtggcacacc ttcacgacta tgagagagcc ccagtgcgtg
     1981 gaaagaggct acccggggtt gttctgggac gctagcctgt ctcgagagcc tcgttgatct
     2041 tcgcgatgac cgcgtccgga tccgagacct caacgatcag gcgatcgtag gtctcgtctt
     2101 tgaggtagat cacgatggtc ttgtcccgat gaccgtgcac gtcgtagaac cggacgccgg
     2161 gcaggtgcac cccgggcaca cgccatcccc tccacagcgt gtgctcgatg ttcgggtcgg
     2221 cttcggcccg ttccacgtgc tccaggggga cacgcagatc gctcaagaag gtcatcgcca
     2281 gctctttgcc cctgacgctt atcacgaggt ggtcgccgtg aacatctacg tgggttggaa
     2341 tatcctcggt ctgggctacc cggtccatga cctcgtgaac cctggcattc gccacggccg
     2401 cctccgtgcg cagcctgggg agggcttcca gcgcctcctc atgggtcatg tccttgatgg
     2461 gcttgttcgc ttccgccgac atggttctcc tccttatcgt ccctttcgac cggaaataat
     2521 gccacgtggc gccgtccggt gcatcccaac gactccttcg ccccggcgta caacccactt
     2581 gtggccacgt tcacccgatc gtgtcgcaac caaaaggtac aatcgtccct cgtgtctgaa
     2641 gccaggaaca gggctgctgt tgcgacggag atctccctgg tcttcgccgc catcttctgg
     2701 gggctgaact tcgcggccac caagtacgcg acggcaacca tcccgccgct cgcgttcgtc
     2761 accctgcgtt tcgctgccgg cggcctcttg ctgctcctcg tactgcgtct tctcgagccc
     2821 aagagcaaac tcaggagggg tgacctgctg ccgatggcgg ccctgggctg cctcggggtg
     2881 gcgacggccc agacggggtt cacgtacggc gtgagcctta ccacggcagg tagcaccggc
     2941 ctgatcttcg cgacggcccc ggtatggggt ttgctcctcg gggcggttct cggcctggag
     3001 cggccgacat ggaaaggcgt ggccggtgtc gggctctcca tcgtgggcgt cgccctggtc
     3061 gttctggacg gcctgctggc ggggcacgcc agcctggtcg gggacctgct ggtcctggtc
     3121 gcggccctgt gcgtgggcgc ctacgcggta ttctccatgc ccttgcttga gcaccacacg
     3181 ccgctcgccg tcgcgaccta tcccgtgctg ttcgggattc ccctcctgtt gatactctcc
     3241 tccccgcaac tcctgagcct ggagtgggga gacgtggggg ccgggccgtg ggccgccgtg
     3301 gcctacgccg gcgtgttcgc cacggcgttc tcgttctctg cctggcaggg tggtatcagc
     3361 cgcatcgggg ccaacagggt gctcgtctac cagtacctca tcaccgccgt tggggtcacc
     3421 tcgggcatcg tgttcttcgg cgaagcgctc agcgtcaaca aggtcgtcgg gggagccgtg
     3481 atcctggtcg gcgtctatct cgcgcgaagg caatgaaagc tatcagctgt cagctttcag
     3541 ctcttatcgc catctgacac gcaccatctc ccggcttaac ctgcatcgct cgttcgtgac
     3601 ctgaatctcc cgcttttctc ccgggacgag atctatgtag ttgtccgaga aatgggtcgc
     3661 ttcgtgggag acctccaggt ttacgaagta ggcgtatgag ggtgcctcca gccttacgga
     3721 gagctggtgg tcgcctttcg gaacgatctc cacgtgtggt tcgacgggct ccctctccag
     3781 gtctttgata ggggcgaaga agtggcggtt ggacgggaaa tggtccccgc tggaggttac
     3841 tgagaggtag cggtcagggg cgccggatgc ttgctctgcg gtccagcggg ctacgggccg
     3901 gctggcgttg gaagagacct cgacgctgtg gttctcttcc cagatcttct ccccgccgaa
     3961 ggttgcgagg tgtaccgtga cgcggtccct catcagcgag agggtgtcgt tggtgagcca
     4021 gagttctacg gagccgtcgg gcagcatctt gaaggaggcg agaagcgggg agtaggccct
     4081 ccgggcgtaa aagtatccgg ccttgccgaa gccgttatgg tcgaggacgc tccacgagag
     4141 gactggccag cagtcgttta gctgccagat cagggtgccc gagcagtggg gcttgcggcg
     4201 cctgaagtgc tccagcgcga acttcaggcc ctcggcctgt gagatcatgc tgaagtccac
     4261 gtactcgtcg aggtctcggg gcaaacccgt cacggtcgac atcaggttgt cgcccttgtg
     4321 cttgggatca tccttgttgt gatggtccat ggagggactg tgatgataga gctggtcttc
     4381 gggtatggcg cgcctgaggg tctcacggac gggcgaggcg tgcatcccga actcgctgat
     4441 aaacctccca gagtcctctg cgtagcggag gtaggagacg ctctcgggag cggtgttgcg
     4501 ccctggttgt tggccgaagt ggcgggggaa gttgccgtgc cagacctccc agttgtgaac
     4561 gttgccctgg cggcggtcgt tgtggtcgtc gccgccgaag gggctaccag gccagtacgg
     4621 ggtgcggcca tcaagctcgg cgacgacgcg cggcaagacc tcgtggtagt agagggtgcc
     4681 ggggaccgtg gtgtcatccc ggtcccagta ggttcggtcg tggatccatt ggttctcgtt
     4741 gttgccgcag aagagggcga ggcaggcgtg tccccgcagg cgtctgacct ggtagcgggc
     4801 ttcttcttcg acctcggcga cgaaagaggc cggttcctcg gggtagaccg cgcaggcgaa
     4861 catgaagtcc tgccagacca ggatgcccag cgagtcgcag agctcgtaga aggcgtcgtg
     4921 ctcgtagatg ccgccacccc agacgcgcag catgttcatg tttgcatcgc gggccgcggc
     4981 gatcaggtta tcgtagcgct cgcgcggtat ggcgccgacg aaagagtcgg cggggatcca
     5041 gtcggccccg cgggcgaaga tggggacccc gttcaacacg aagcggaaga aacgggtccc
     5101 tggctcgtcg gggtcagggg actggtcaag ctctagcgtc cttatgccta ccttcgaggc
     5161 gtgcgtgtcg agcacgtctc catcctgacg cagggctact tcgaggccgt atagcgaggg
     5221 ccggccaagg tcgtgggtcc accacaggca cggtctctcc acgctaacgt aggccgtgcc
     5281 cgtgaggtcg gcgccttctc cttcgagcgt gagcgttgcc ccagaaacag gctccccgcc
     5341 gggtgcggtt agcgagatgg aggcctcgat cgcgccctcc gccgcgaacc tgtcggcctc
     5401 gacccgcacg gcgacggcga cccgctctcc cgacggggcg atctccaggg tatggaaatg
     5461 aactcccctg atgcgggcgc gacgttcccg ccgcagctcg acgggacgcc agatcccgac
     5521 agtcggaagc ctcggccccc aatcccaccc gaagccgaac tgggccttgc gcatggagac
     5581 ccgctccggg ttcctgcccc actgatcggg gtgc
//
LOCUS       DBNGVD010000306         8971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_799658, whole genome shotgun sequence.
ACCESSION   DBNGVD010000306 DBNGVD010000000
VERSION     DBNGVD010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_799658"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1477
                     /locus_tag="AC16CM_08100"
     CDS             278..1477
                     /locus_tag="AC16CM_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR3139832.1"
                     /translation="MRNLSGAKHPATKAVWAGEERYLVEGATQVPIVQSIPFGYDDLD
                     EWTRVALGEREGHIYSRNTNPTTHAFEEKVRTLEGAEAATSFATGMAAVSNTLLEFLQ
                     PGSRVVSVKDTYGGTNKVFLEFLLRAGVEVRLCDTTDHDAIAKEVARGCNLLYLETPT
                     NPTLKLVDIKRLAKVGHEAGAVVVVDGTFATPINQSPLSLEADLVIHSASKYLGGHSD
                     ALGGVLCGSDALVKRVYHYREINGAALDPFAAYLLLRGLKTLDLRIARHNENAMQVAR
                     FLEQHPGVERVFYPGLESHPQHELARRQMRGFGGMLSFSLRGGYEAVRTFLPRLQFAH
                     RAANLGAVETTVGPPATTSHVELSASERREMGIPESLIRYSTGIEHPDDLISDLENAL
                     STTHAEN"
     gene            1771..2700
                     /locus_tag="AC16CM_08105"
     CDS             1771..2700
                     /locus_tag="AC16CM_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139833.1"
                     /translation="MVGLPLTIVVTVYIALRSDPSEPEILPIAAPVLVWVVGLLILQW
                     TSLLTDGVGPAAPEGEEPQAGTADPPLDREALHAVLRVDGGHVSSAPTSRSFAVRGFL
                     LFTAFTIALPAGALLYITGIVGATISPLGVGGPAIPVAVLPGLALVAFGVLRLAGQYR
                     QATRVADSYLKDLGLRLDGPSPPVSVAHPKGATARPPGGLAYAGERHGWPVRIEQSAG
                     ASSVHVANPMPPFVARELDLALSPEPGTPPAVVDALAELSPHPRWANVRVTAGDDGVL
                     VERQVDASMAYRKLWLDDLWLAERLVSVSDNRI"
     gene            2747..3484
                     /locus_tag="AC16CM_08110"
     CDS             2747..3484
                     /locus_tag="AC16CM_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139834.1"
                     /translation="MDTGTSVGAPLLLALELTVLYAAGSYAMGRLVSLLGAGGILGRS
                     AFYLLVFPGVVLHEGAHYLACLLTGTRVVRFAPFAPQRTADSRLVLGYVRHEQRALPI
                     RAIIGLAPVVLNPLGLLLTTALLTPLTFRQVADPSAGVVVREIFTGGFLTGSPIQATV
                     WAYLSLSFALGSVPSREDLSGLPLLLAVLGVGILVAGSLRLGTEGVLASAAYDLSALA
                     AGLYALPAAVAAGAAIVTGLLAAPGQR"
     gene            3585..3962
                     /locus_tag="AC16CM_08115"
     CDS             3585..3962
                     /locus_tag="AC16CM_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3139835.1"
                     /translation="MSEDRCDLICIDAPRAEAIRGRLLGEEVALGAAERAKALSDPTR
                     LTLAAALREAGEGGELCVCDLSWIAGRSQNLVSHHLRQLRSHGLVRSRRNGKLVMYSL
                     SDEGASLLSAVLGRSLVGEKVGT"
     gene            3959..6247
                     /locus_tag="AC16CM_08120"
     CDS             3959..6247
                     /locus_tag="AC16CM_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3139836.1"
                     /translation="MNRPRKMLPVLGQEAQGPKETEEGANDAAGCTDDCCQSPEVIIP
                     STGGSELEEPEGGVASGVLERTVLRVEGMDCASCATTVERRVGGLPGVRSATVNFAAG
                     RLDAEHDSGLALEEIEAAVRAAGYGVAETEEVERPPFWRTPRALSVSASALFFILGLA
                     LTLTGAPEYTRVAAYLSAIVIGGVPIFRAAIVGLRARHMDMNVLMSVATVGAAGIGQW
                     AEAASVVVLFAAGNALQVYAIDRTRGAVRALAGLAPDEVLVRREGSEVIVPAEEVEVG
                     ETVVVRPGERLAVDGRVVEGSSTVNEAPVTGESVPVEKGAGSVVYSGSVNGQGGLQVE
                     ATKRAGDSTLQNVARLVEEAQASKAPSEQFVDRFSRIYTPIVVVAAVVLAVVPPVLGA
                     GFGEWFYRALALLIIACPCALVISTPVTVVSGIGAASRRGILLKGGEALEAAGRLKAL
                     AFDKTGTLTEGRPVLSRVVALDGSDEASVLALAAALERRSEHPLAHAILSAGAGRAEE
                     GSSLPPVANFRSVAGRGAEGEVGGKRYLIGSPRMFAEREVPLEDASETLEEIERAGET
                     PVLLGTHGGPLAVFGLADSIRPDAKATVEALRASGVEELVMLTGDAEAPARRVAEELG
                     IGYRAGLLPEEKIEAVRGLVATHGDAGMVGDGVNDAPALAVSSVGFAMGAAGTDVALE
                     TADVALMRDDLSKLAEAVALSRAAEGIIRQNVAVSILIKGLFVLLAPFGLVALWMAVL
                     ADMGTSIAVTLNGLRLFRKGRR"
     gene            6306..6950
                     /locus_tag="AC16CM_08125"
     CDS             6306..6950
                     /locus_tag="AC16CM_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIR3139837.1"
                     /translation="MSARNVAYALLLVLAVAVAAGALFLWASSRPPQEGSAEAGFARD
                     MMVHHAQAVEMAEIVRDKTENPQIRTLATDISLTQQAQIGQMQGWLAVWGLSPTGTEK
                     PMAWMGHPVNGPMPGMASQEEIDRLGTLPPEEADELFLRLMIPHHEAAIPMARAIEDR
                     SDDQVVDLFAEKVAATQRVEVKNMQKMLEKMGASPPKDEPSMEGMDMGEDHHGG"
     gene            complement(6954..7202)
                     /locus_tag="AC16CM_08130"
     CDS             complement(6954..7202)
                     /locus_tag="AC16CM_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139838.1"
                     /translation="MRKIVGSVGRSGEFDAWFFPACSCTADRWRSAYGALLEGKTLPP
                     VELYKLGGAYFVVDGNHRVSVARWRGMVAVDAVVTEFV"
     gene            complement(7221..7439)
                     /locus_tag="AC16CM_08135"
     CDS             complement(7221..7439)
                     /locus_tag="AC16CM_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139839.1"
                     /translation="MICNQERTGVYSRGGAEREEEGGRGFRRARRAAYLSCLGNRIRW
                     RFFGTAPSCESLRSFEEELAGRGLERKD"
     gene            7497..8354
                     /gene="arsM"
                     /locus_tag="AC16CM_08140"
     CDS             7497..8354
                     /gene="arsM"
                     /locus_tag="AC16CM_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR3139840.1"
                     /translation="MGVGNEELRGRVRERYAVAAMSVTEGSGAASCCGSAGEPGHDSE
                     ALGEDWTGGGYSAEELETLPQAAGAASLGCGNPTALATLSAGEVVLDLGSGGGIDVLL
                     SAKRVSPGGKAYGLDMTDEMLALAERNRAESGAENVEFLKGHIEEIPLPEDHVDVVIS
                     NCVINLSTDKPRVLSEAFRVLKPGGRFAVSDMVFLGDKGKLPAEVVQSIEAWSGCVSG
                     ALEKEEYEGLLREAGFEDVSIEVTHTYDAELAAAGSTSGCCGDSGCCGDTATADVELA
                     SAFIRGRKP"
     gene            8351..8779
                     /locus_tag="AC16CM_08145"
     CDS             8351..8779
                     /locus_tag="AC16CM_08145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3139841.1"
                     /translation="MSREQIGVLEENGVARTESAGDAGVERLVALGRALSDPIRVRML
                     SMMADGRGCCDLSGSEVPAGEGQEGICVCEFEEQFGMGQSKVSYHMRKLKDAGLVSEE
                     KRGRWSFYSLDREAAGGLLGEAADRLLSGPEKREAAGGCC"
     gene            8766..>8971
                     /locus_tag="AC16CM_08150"
     CDS             8766..>8971
                     /locus_tag="AC16CM_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR3139842.1"
                     /translation="MAAAEASRTGRVLFLCTHNSARSQMAEGLLRHLAGDSFEVRSAG
                     TEATHVRPLAIRAMGEIGVDISGQ"
BASE COUNT         1468 a         2909 c         3112 g         1482 t
ORIGIN      
        1 gggagaaccg tgaatgaaca acacgggcga ccctccaccc tcgtcgctgt actggacagg
       61 accgttggcg gtgtcggcga tggtggcgtc aggtcggttc tgggatggca acgagtctcc
      121 tcctcgattc gggcaggcga cggtgggatg gttccaggat gctcctggcc gcgcaacgcc
      181 gcgaacacat caccgtaagc tctgtattgt actgggcaca ccggcgagag ttgaacgggg
      241 cgctcgggga gaacgcgcac ccaggaggag gaaggtaatg aggaacctgt cgggagcgaa
      301 acacccagcg acgaaggccg tttgggccgg ggaggaacgc tacctcgtcg agggcgccac
      361 ccaggtgcct atcgtccaga gtataccgtt cggctacgat gatctcgatg agtggacgcg
      421 cgtcgcgctc ggtgagcgtg agggccacat ctacagccgc aacaccaacc ccaccaccca
      481 cgctttcgag gagaaggtga ggacgctaga aggtgcggag gccgccacga gcttcgccac
      541 cggcatggca gccgtcagca acacgcttct cgagtttctc caacccggca gccgcgtcgt
      601 ctccgtgaag gacacctacg gcgggacgaa caaggtcttc ctggagttcc ttctcagggc
      661 tggtgtggag gtgaggctct gcgataccac ggaccacgac gctatcgcga aggaggtcgc
      721 cagaggctgc aatctcctct acctggagac accgacgaac cccacgctca agctggtcga
      781 cataaagcgg ctcgcgaagg tcgggcacga ggccggcgcc gtagtggtgg tcgacggcac
      841 cttcgccacg cccataaacc agagcccact ctccctggag gccgacctgg tgatccacag
      901 cgcctccaag tacctcgggg gacactcgga cgccctcggc ggcgtcctgt gcggatcgga
      961 cgccctcgta aagcgggtct accattaccg ggagatcaac ggcgccgccc tcgatccctt
     1021 cgccgcctac ctgctcctgc gcgggctgaa gaccctggat ctccggatcg cccgccacaa
     1081 cgagaacgcc atgcaggtcg cgcgtttcct ggagcaacac cccggcgtag agagggtctt
     1141 ctaccccggc ctggaatccc atcctcagca cgagctcgcc aggaggcaga tgaggggttt
     1201 cggggggatg ctgagcttct cgctccgtgg cggctacgag gcggtccgga ctttcctgcc
     1261 ccggttgcaa ttcgcccacc gggcggcgaa cctgggtgcc gtcgagacca cggttgggcc
     1321 acccgccacc accagccacg tcgagttgag cgcgagcgag cgcagggaga tgggcatccc
     1381 cgagtcgctc atcaggtact ccacaggcat agagcaccct gacgacctga tctcggacct
     1441 ggaaaacgcc ctgtccacaa cgcacgccga gaactgaaga ccccgcacgc gtagattccg
     1501 cacctggcgt gccggggaag gccgatctgg aacaggtcga tatccatgaa acctgccttg
     1561 gccatagcct cggccacgcg aagaaagtag gatatcggca ggaggaagcc ccgtagatac
     1621 gaccgctgac aagatgcaag tcacgccctg cgaagttcac gagtgcctct ggtgcgtaga
     1681 gacgtggatg ccgtccttcg caggccccgt agaagcggat actcggacgt gaacatcggg
     1741 gggcgttcct ggatatacct cctttacgtc atggttggtc tgccgctgac gatcgtggta
     1801 accgtctaca tcgcgctgag gtccgaccct tccgagccgg agattctgcc catagccgct
     1861 cccgtgctgg tgtgggtcgt ggggctcctg atcctgcagt ggacctctct cctgaccgac
     1921 ggggtgggcc cggccgcacc ggagggggag gagccgcaag cgggaacggc cgaccccccg
     1981 ctcgaccgcg aggcgcttca tgcggtgcta cgcgttgacg ggggacacgt gtcgtcggca
     2041 cccacttccc gatcctttgc cgtgaggggc ttcctccttt tcacggcctt cacgatcgcg
     2101 ctccccgcag gtgccctcct gtacataacc ggtatcgtcg gggccactat ctctcctctt
     2161 ggcgtgggcg gacctgcgat accggtggcc gtactcccgg gcctcgcgct cgtggccttc
     2221 ggtgttcttc gcctcgcggg ccagtaccgc caggccacca gggtggccga ctcgtatttg
     2281 aaagacctgg ggttgcgcct tgacggtcct tcccctccgg tcagcgtcgc ccaccccaag
     2341 ggggcgaccg cgcgtccccc gggaggtctc gcctacgcgg gggagcggca cggatggccc
     2401 gtgcggatcg aacagtctgc gggagcctcg tcggttcacg tcgcgaatcc catgccgcct
     2461 ttcgtggcgc gcgagctcga tctagccctc tcacccgagc cgggtacgcc ccccgccgtc
     2521 gtagacgcgc tcgccgagct gagcccgcat ccgcgctggg caaacgttcg tgtcacggcc
     2581 ggcgatgacg gggtgctcgt cgaacggcag gtagacgcga gcatggctta tcggaagctc
     2641 tggctcgacg acctctggct cgccgagcgc ctcgtatcgg tctcggataa tagaatctga
     2701 ccggagatgg atctatggat cttccgagaa gcatgaccgg agcgtgatgg acaccgggac
     2761 gtcggtgggg gccccgctcc tgctcgccct ggagctgacc gtcctctatg cagccggctc
     2821 gtacgccatg ggccgcctgg tctccttgct cggggccggg ggtatcctgg gtcgatcggc
     2881 gttctacctc ctggtctttc cgggcgtggt gctccacgag ggtgcccact acctggcgtg
     2941 cctgctcacc ggtaccaggg ttgtccgatt cgcgcccttc gcaccacaga ggacggcgga
     3001 cagccggctg gtgctcggct acgtccgcca cgagcagcgc gccctgccga taagggcgat
     3061 catcgggctc gcgcccgtgg ttctgaaccc gctgggactc ctgctcacga ccgcgctgct
     3121 cacgccgctt acgttccgcc aggtggccga cccgtctgcc ggggttgtag tacgggaaat
     3181 ctttacaggc ggcttcctga ccggctcccc gattcaggcc accgtgtggg cgtacctgtc
     3241 gctgagcttc gcgctcggaa gcgtcccgag cagggaagac ctgtcgggcc tgccgctcct
     3301 gctggcggta ctcggcgtgg ggatcctcgt agcaggctcc ctgcggctcg gcacggaagg
     3361 cgtcctcgcc tcggcagcct acgacctgtc cgcgctggcg gcgggactct acgccctgcc
     3421 agccgcggtg gccgcgggtg ccgccatcgt cacgggcctc ctggcagcac cgggccagcg
     3481 ttaaagcccg accagtttcg tgtaccctcc tcggcccggc tgcgtcaccg aacaccacgg
     3541 actgctatac tcggcctaac atatcaacgg gtgttggagt gtatatgagt gaggaccgct
     3601 gcgacctgat ctgcattgac gccccgcggg cggaggcgat acgcgggagg ctgcttggcg
     3661 aggaggttgc ccttggggcc gccgagcgcg ccaaggcgct ctccgatccc acgcgcctga
     3721 cgctcgccgc ggccctgcgc gaggctggcg aaggtgggga gctctgcgtt tgcgacctct
     3781 cctggatagc cgggcgctcg cagaacctcg tctcacacca cctcaggcag ctgagatccc
     3841 acggcctcgt tcgctccagg aggaacggca agctggtcat gtactcgctc tcggatgagg
     3901 gcgcctcgct cctctcggcc gtgctgggta ggtcactggt aggtgagaag gtagggacgt
     3961 gaatcgcccg agaaagatgc tccccgtcct ggggcaggaa gcgcaggggc cgaaagagac
     4021 ggaggagggg gccaacgacg cggccggctg cacggacgac tgctgccaga gcccggaggt
     4081 catcatcccg agtacagggg ggtctgagct agaggaacct gagggtggtg tggcgtccgg
     4141 cgtcctggag aggaccgtct tgcgcgtcga ggggatggat tgcgcgagtt gcgcgaccac
     4201 cgtggagagg cgcgttggcg gcctccctgg cgtccgttcg gcgaccgtga actttgccgc
     4261 tggcaggctc gatgcagagc acgactccgg cctggcccta gaggagatcg aggccgccgt
     4321 cagggccgcc gggtacgggg tggcagagac ggaggaggtc gagaggcctc cattctggcg
     4381 caccccgagg gccctgtctg tctctgcctc ggccctcttt ttcattctcg ggctagccct
     4441 gacgctcacc ggggcgcccg agtacacgcg cgttgcggcc tacctctcgg ccatcgtgat
     4501 cggtggggtg ccgatcttcc gggcggccat agtcgggttg agggccaggc acatggacat
     4561 gaacgtcctg atgagcgtgg ccacggtcgg ggctgcgggg ataggacagt gggcggaggc
     4621 cgcctctgtg gtggtcctct tcgccgcggg gaacgcactt caggtctacg ccatcgaccg
     4681 aacgcgcggg gccgtgaggg ccctggcggg gctcgccccg gacgaggtgc tcgtcaggag
     4741 ggaaggctcc gaggtaatcg tcccggcgga agaggtcgag gtcggcgaga cggttgtggt
     4801 gcggcccggc gagcggcttg cggtagacgg cagggtcgtc gagggatcct ccacggtcaa
     4861 cgaggccccg gtcacgggtg agagcgtgcc cgtcgagaag ggtgctggca gcgtcgtata
     4921 ctctggctca gtgaacggcc agggggggct ccaggtcgag gcgacgaaac gggccgggga
     4981 ctcgacgctg cagaacgtgg cccgcctcgt ggaggaggcc caggcgtcca aggccccttc
     5041 cgagcagttc gtggaccgct tctcgcgcat ctacaccccc attgtggtgg tcgcggccgt
     5101 ggtgctcgcg gtcgtgcccc ccgttctcgg tgcgggcttc ggtgagtggt tctacagggc
     5161 gcttgcgctc cttatcatcg cctgcccgtg cgccctggtc atctccacgc ccgttacggt
     5221 ggtctcaggc atcggggcgg cttccaggcg gggcattctg ctcaagggtg gtgaggcgct
     5281 cgaagccgca ggtaggctca aggcgctcgc cttcgacaag accggtaccc tgacagaggg
     5341 acgccccgtg ctctcgcgtg tcgtggcgct agacggtagc gacgaggcct cggttctggc
     5401 gctggcagct gcgctggaga ggagatcgga gcatccgctg gcgcacgcca tcctctccgc
     5461 cggagccgga cgtgccgagg aaggatccag ccttcccccg gtcgcgaatt ttcggtcggt
     5521 ggcgggtagg ggagcagaag gcgaggtggg aggaaaaagg tacctgatcg ggagcccccg
     5581 catgttcgcc gagagagagg tacctctgga ggacgcctcc gaaacgttgg aggagatcga
     5641 acgcgccggc gagaccccgg tcctccttgg cacccacggc ggtcccctgg cggtcttcgg
     5701 cctcgccgac tccatccgtc ccgacgcgaa ggcgaccgtc gaggccctgc gcgcgtcggg
     5761 ggtcgaggag ctcgtgatgc tgactgggga cgccgaggcc ccggcccggc gcgtggccga
     5821 agagctcggc atcggttacc gggcggggct cttgcccgag gagaagatag aggccgtgcg
     5881 cgggctcgtg gcgacccacg gcgacgccgg tatggtcgga gacggcgtga acgacgcgcc
     5941 ggccctcgcc gtctcctccg tcggtttcgc catgggggca gccggtacgg acgtggcgct
     6001 agagacggcc gacgtcgccc tcatgcgcga cgacttgtcg aagttggccg aggccgtcgc
     6061 gctctcgcgc gccgcagagg gtatcatcag gcagaacgtg gccgtctcga tactcatcaa
     6121 gggtctcttc gtgctgctgg cgcccttcgg gctggtggcg ctctggatgg ccgtcctcgc
     6181 ggacatgggc acctccatcg cagtcaccct gaacggcctc aggctcttcc gcaagggtag
     6241 acgctgatag tgcccccggc tggacgactc ggcgcgggcg ccgccccgcc gatagcacgt
     6301 tcgcgatgag cgcacgaaac gtcgcctacg cgctgttgct cgtcctcgcc gtcgcggtcg
     6361 ccgccggggc cctgttcctc tgggcctcat cccggccgcc acaggagggg tcggcggagg
     6421 ccgggttcgc ccgcgacatg atggtccacc acgcccaggc cgtggagatg gccgagatcg
     6481 tgcgcgacaa gaccgaaaac ccacagataa ggacgctcgc caccgacatc tcgctgaccc
     6541 agcaggccca gatcggccag atgcagggct ggctcgccgt gtggggcctc tcgccgacgg
     6601 gcaccgagaa accgatggcc tggatgggcc accccgtgaa cggccccatg ccggggatgg
     6661 cctcccagga agagatagac cgcctcggca cgctgccccc cgaggaggca gacgagcttt
     6721 tcctgcggtt gatgatcccc caccacgagg cggccatacc aatggcccga gcgatagagg
     6781 accgctccga cgaccaggtg gtggacctct tcgccgagaa ggtcgcggcg acccagaggg
     6841 tggaggtaaa gaacatgcag aagatgctgg agaaaatggg cgcctccccg ccgaaagacg
     6901 agccctccat ggagggcatg gacatgggag aagaccacca cggcgggtaa tccctaaaca
     6961 aactccgtga ccaccgcgtc cacggccacc ataccccgcc agcgcgccac cgagacccgg
     7021 tggtttccgt ccaccacgaa gtaggcgccc cccagcttgt agagctccac cggcggcagc
     7081 gtcttgccct ccagcaacgc cccgtaggcg ctcctccacc ggtctgcggt gcacgagcag
     7141 gccgggaaga accacgcatc gaactcgccg ctcctgccca cgctgcccac gatcttgcgc
     7201 acctcgacgg tctcgaagcc tcaatccttg cgctcgagac cgcgacccgc cagctcctcc
     7261 tcgaaggatc tcaaggactc gcagctcggc gccgtaccga agaacctcca ccttatcctg
     7321 ttgcccaggc aagacagata cgctgcccgc ctcgcccggc ggaagccccg cccgccctct
     7381 tcttcccgct ctgctccgcc tcgcgagtac accccggtcc tctcctggtt gcatatcaac
     7441 ttttcttgtt gtactgtata tcgaagattg ttgatgcgag cgaggaggac gtaaacatgg
     7501 gcgttgggaa cgaggagttg agggggcggg tcagggagag gtatgcggtg gcggccatgt
     7561 ccgtcaccga gggctctggt gccgcctcgt gttgcgggtc ggcgggcgag cccggtcatg
     7621 attccgaggc gctcggcgag gactggaccg ggggcggata ctctgccgag gagttagaga
     7681 cgctgccgca ggcggcgggc gctgcgtcgc tcgggtgtgg caacccgacg gcgttggcga
     7741 cgctctctgc gggggaggtg gtgcttgacc ttggcagtgg gggcggcata gacgtgctgc
     7801 tctctgcaaa gagggtctcg ccgggtggga aggcctacgg gctggacatg accgacgaga
     7861 tgctcgcgct cgccgagcgg aacagggccg agtcgggggc ggagaacgtc gagttcctga
     7921 agggccacat agaggagata ccgctgcccg aggaccatgt cgacgtggtg atctccaact
     7981 gcgtcatcaa cctctccacc gacaagccca gggtgctctc agaggccttc cgcgtcctga
     8041 aaccgggcgg gcgcttcgcg gtctcggaca tggtgttcct gggcgacaag gggaaactgc
     8101 ctgccgaggt ggtacagagc atcgaggctt ggtccggctg cgtctcgggg gcgctcgaga
     8161 aggaggagta cgaggggctg ctgcgggagg ccggcttcga ggacgtctcc atagaggtca
     8221 cccacaccta cgatgccgag ctcgcggcgg ctggttccac ctccgggtgc tgtggggact
     8281 cgggttgttg cggggacacg gcgacggcgg atgtcgagct tgccagcgcc ttcatcaggg
     8341 gccgcaagcc gtgagccggg agcagatcgg ggtcctggag gagaacggcg tcgcccgcac
     8401 ggaaagcgcc ggggacgccg gggtggagcg cctcgtcgcc ctgggtaggg ccctctccga
     8461 ccccatcagg gtaaggatgc tctccatgat ggccgacggc aggggatgct gcgacctctc
     8521 cggctcggag gtgcctgccg gagaaggcca ggagggcatc tgcgtctgcg agttcgagga
     8581 gcagttcggc atgggtcagt cgaaggtctc ctaccacatg aggaagctaa aggacgccgg
     8641 gctggtcagc gaagagaagc ggggccgctg gagcttctac tccctcgata gagaagccgc
     8701 tggaggtctc ctcggcgagg ccgccgaccg gctgctctcc ggccccgaga agcgagaagc
     8761 tgccggtggc tgctgctgag gcctcgcgga cgggcagggt ccttttcctg tgcacccaca
     8821 actccgcgcg ctcgcagatg gcggaggggc tgctgaggca tctggcgggg gatagcttcg
     8881 aggtcaggag cgcgggcacg gaggccaccc acgtaaggcc gctggcgatc agggcgatgg
     8941 gggagatcgg cgtggacatc tcggggcaag a
//
LOCUS       DBNGVD010000307         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_616761, whole genome shotgun sequence.
ACCESSION   DBNGVD010000307 DBNGVD010000000
VERSION     DBNGVD010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_616761"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16CM_08155"
     CDS             <1..290
                     /locus_tag="AC16CM_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3139843.1"
                     /translation="LLFENQGEENSGYVTDDFLRGLAEDTPGLDVKKWEDARAGESFT
                     RELEAVQAKAQSEGVNSTPTLIFEGPGGQVKIDRLTDYDQITSAVDKADGS"
     gene            280..723
                     /locus_tag="AC16CM_08160"
     CDS             280..723
                     /locus_tag="AC16CM_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3139844.1"
                     /translation="MAPSVRSGRRYTAPRVALLALAVVGVLISAYLTWTHFAGLTPVC
                     TGSGEGCQTVQSSRYASLLGIPVAVLGLVAYGGLVFSVAVWAEIGIFLSFLISLVGTL
                     FSVYLTYLEIFVIGALCQWCLASAVIMVAALVCATLAVLPLLRAR"
     gene            complement(755..2146)
                     /gene="argH"
                     /locus_tag="AC16CM_08165"
     CDS             complement(755..2146)
                     /gene="argH"
                     /locus_tag="AC16CM_08165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3139845.1"
                     /translation="MSERDNSPLWGGRFGASPAEAFERLNASIPFDIRLAPYDIRGSI
                     AHARMLGVQGIVSAEESATLVRGLEAVLAEVEAGEFAWDLSDEDVHTAVERRLREVAG
                     DVALKLHTGRSRNDQVALDTHLFARDAADEIRGAVLATMRALVEVAQKERGLILPGYT
                     HLQRAQPILLSHHLLAHFEALKRDVRRLEAARGAANVSPLGAAALAGTPHPVDPDLTA
                     RELGMEPLSNSLDAVSDRDFALDLLYACAVLGLHLSRMGEEWVLWTSAEFAFATLDDA
                     YSSGSSIMPQKKNPDAYELMRGRAGRLIGDLNALLITLKSLPLGYSKDLQEDKEPLFD
                     AVDAVLPMLAVLPEMLRTARFHGERMEAAAGGFALATELADFLASNGVPFRDAHHAVG
                     RLVKRCEELGSSLEDLPLEELAEAHPALARLPEGLLSPRGSVRNKRSSGSTSPDAVDT
                     QLGRAREFLDAAG"
     gene            complement(2201..>2609)
                     /locus_tag="AC16CM_08170"
     CDS             complement(2201..>2609)
                     /locus_tag="AC16CM_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR3139846.1"
                     /translation="KSRVALLVEHVESPRHKGALESADVVVPGGSPECGGSVTVYLQG
                     DGNGGVEALTWTGQGDTISMGSTSIVVDRIVSEKLTMDEILAEDYDQLVDLIGRDIIR
                     NRKRHATLGLSTIKAAVRQYQKKIRTTQPKTRS"
BASE COUNT          421 a          860 c          858 g          470 t
ORIGIN      
        1 cgctcctctt cgagaaccag ggagaggaga acagcggcta cgtcacggac gatttcctgc
       61 ggggcctggc cgaagacaca ccggggctgg acgtgaagaa gtgggaagat gcgcgcgccg
      121 gagaatcctt caccagagag ctggaggccg tccaggcaaa ggcacagtcg gagggcgtaa
      181 actccacgcc gaccctgatc ttcgaaggac caggaggtca ggtcaagata gaccggctga
      241 ccgactacga ccagatcacc tcggccgtag acaaggcgga tggctcctag cgtcaggtcc
      301 gggaggcggt atacggcccc ccgtgtcgcg ctcctggcgc tggcggtggt gggggtcctg
      361 atcagcgctt acctcacctg gacccacttc gccggcctga cccccgtctg tacaggaagc
      421 ggggaaggct gccagaccgt gcaatccagc cgctacgcct cgctcctggg gatacccgtg
      481 gccgtgctcg gcctcgtcgc ctacggcggc ctcgtcttct ccgtggccgt atgggccgag
      541 atagggatct tcctcagttt cctgatctcg ctcgtcggga cgctcttcag cgtctacctg
      601 acctacctgg agattttcgt gataggagcc ctgtgccagt ggtgcctggc gagcgcggtg
      661 atcatggtcg cggccctggt ctgcgcaacg ctcgccgttc tgccgttgtt gcgagcaagg
      721 tagctcaccc ctggcccgcg agggttaccc gggctcagcc cgcggcgtcc aggaactcac
      781 gggctcgccc taactgggta tcgacggcgt caggggacgt ggagcctgat gaccgcttgt
      841 tccgtacgct gccgcgcgga gagagcaacc cttctggcag ccgggcgagc gcgggatgcg
      901 cctccgcgag ctcctccagg ggtagatcct ccagcgagga tcccagctcc tcgcagcgct
      961 tgacgagcct tcccacggcg tgatgcgcgt ccctgaaggg gaccccgttc gaggcgagaa
     1021 agtcggcgag ctcggtggcg agggcgaagc cgccggcggc cgcttccata cgctcgccgt
     1081 ggaaccgggc cgtgcgcagc atctcgggca gcacggcgag catgggcagc accgcgtcca
     1141 cggcgtcgaa gagaggttcc ttgtcctcct ggaggtcctt cgagtacccc agcgggaggc
     1201 tcttcagcgt gatcagaagc gcgttcaggt ccccgatcag gcgcccggct ctaccgcgca
     1261 tgagctcata ggcatcgggg ttcttcttct ggggcatgat cgacgaaccg gaggagtagg
     1321 cgtcgtcgag ggtggcgaag gcgaactccg cgctggtcca aagcacccac tcctccccca
     1381 tcctgctgag atgcaaccct agcacggcgc acgcgtagag cagatccaga gcaaaatccc
     1441 ggtccgacac ggcgtcgagc gagttcgaga ggggctccat tccgagctcc cgcgccgtga
     1501 ggtcgggatc tacggggtgc ggcgtgcccg cgagggcggc tgccccgagc ggcgacacgt
     1561 tggcggcccc ccgcgcggcc tccagcctcc tgacatccct tttcagggcc tcgaagtggg
     1621 cgagcagatg gtgggagagc agaatgggct gggcgcgctg aaggtgggtg taaccgggca
     1681 ggatgagacc gcgctctttc tgtgccacct cgacgagggc gcgcatcgtc gcgagcacgg
     1741 cgccgcgtat ctcgtcggcc gcgtccctgg cgaagaggtg cgtatcgagg gccacctgat
     1801 cgttcctgct ccgccccgtg tgcagcttga gggccacgtc gccggcaacc tccctgagcc
     1861 tgcgctcgac ggccgtatgc acgtcctcgt cggagaggtc ccaggcaaac tccccagcct
     1921 cgacctctgc gagcaccgcc tcgagccccc gtaccaacgt ggccgactct tcggccgaga
     1981 cgatcccctg caccccgagc atcctggcgt gggcgatgga acccctgatg tcataggggg
     2041 cgaggcggat gtcgaagggt atcgaggcgt tcaggcgttc gaacgcctcg gccggggatg
     2101 ccccgaacct accgccccac aggggactgt tgtctctctc gctcaccacg gtgcagttta
     2161 cagcggatgg ccagagaagt tagcttctag ctctcagccg tcagctcctg gttttcggct
     2221 gcgtcgtgcg gatcttcttc tggtactgcc ttacggcggc ctttatggtg gagaggccaa
     2281 gcgtggcatg cctcttgcgg ttccttatta tatccctgcc gatcaggtcg acgagctgat
     2341 cgtagtcctc ggcgaggatc tcgtccatgg tgagtttctc gctcacgata cgatccacca
     2401 ctatggatgt ggaccccatg cttatggtgt ccccctgccc cgtccaggtc agggcctcta
     2461 ccccgccgtt gccgtcgcct tggaggtaga cggttacaga accaccgcac tcggggcttc
     2521 ctcccggcac cacgacgtcg gcgctttcga gagccccctt gtggcggggg ctctcgacgt
     2581 gttccacgag cagagctacg cggctcttg
//
LOCUS       DBNGVD010000308         5480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2986070, whole genome shotgun sequence.
ACCESSION   DBNGVD010000308 DBNGVD010000000
VERSION     DBNGVD010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2986070"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3261
                     /locus_tag="AC16CM_08175"
     CDS             <1..3261
                     /locus_tag="AC16CM_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3139847.1"
                     /translation="HGDLVHGHVRDLPESEQPELRAEEILSIALVPIFVEGRWWGFIG
                     FDECVQEREWSAAEVGALGAAAGTLGAAIRRRQTEELLRGSEERYRAVIEQATDGIYL
                     LDVETRRFFETNPSFRRMFGYTAQEVGEMDIYDLVAHPREDVDTTIRQTLRSGRRIVG
                     ERKYRRKDGDLLDVEVGVSVISLDGRDVVCTIVRDVTERKRNEEALRGSEAELRALFE
                     AMTDLIFVIDGEGRHLKVALTNPALLYRSHDETLGHTLHELFPKEQADEFLGHVRSVL
                     STRRSVSFEYSLPMELGEKWFEGTVSPMLEDSVVWVARDITERKKSEEALTRSESRLR
                     QIIETEPECVKVLGTNGSLLEMNPAGLAMIEAESLEQVRGRSVYRYIAPDHREAFVNL
                     TEKVLRGGSGTLEFELVGLKGTRRWLDTHAVPLRDAGGEAAGLLAITRDITQRKEAEK
                     RLRDAEFKYRTLVERIPATIYIQVPRSDGASAYAVTYMSPQVEKLLGHPAQRFVEDPR
                     FWNSVTHPDDLQRMVVETGRTDRTGEPFALEYRMLHADGDYRWIRDEAVLVRDEQGNP
                     MFWQGVFSDVTERKRAEDAMQQSERLYRTVIEQATENIFLIDVESRRIVESNHAFREA
                     LGYSEEELHRMTLYDVVAADPESVDINLRRTLKEGRASVGERRYRRKDGTLLDVEAGG
                     SVIVRDGRQTLVAVAHDITERARAQHMLEERVATLSGIAGELTLDRPTETVLGDLARS
                     VVKASTAVACGVVLIGDGEGAVHLFGSYGLPENYTAALGEAYRAGVRSPSLEAYRSRE
                     PVLVHGLRARILGDPLYAPVHRYMREVSWDVVYSLPLVSRGSALGAIFFCFPPAGEPG
                     EDEKIFLRAVADQAAVAVENARLFSEARGKAALEERQRLARELHDSVSQALYGIALGV
                     ETARELLPDHPERAEEPLDYATTLAEAGMTEMRALIFELRPESLEKEGLVAALEKQAA
                     AVQARHGIKVEAEWDREPEATLEVKEALYRVAQEALHNTVKHARATNVKLKLEELPDV
                     FTLGISDDGLGFEPHNDFPGHLGLKSMRERAARLGGTLEVTSEPGHGVRILVSIPR"
     gene            3408..3854
                     /locus_tag="AC16CM_08180"
     CDS             3408..3854
                     /locus_tag="AC16CM_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139848.1"
                     /translation="MRTTAAYGTAVAVLATAVNLLHGISHVGQEVLSLQAWQWAYVIC
                     VIFLAPLVAALLLWSPFRLAGAWLLFGSMVGSLVFDLAYHFLIEGPDNVFTQEPGAWL
                     AAFRVSSVLLVATSGLGALVGGWVLLRFSRPRTLSEAPVPAGRGRR"
     gene            3851..4051
                     /locus_tag="AC16CM_08185"
     CDS             3851..4051
                     /locus_tag="AC16CM_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139849.1"
                     /translation="MNEMNPYAWRQRREDLMREIERDRSGRALGAERKAHTFSPAWEL
                     RRMAGLLLKLIRRLVSRNGAHP"
     gene            4048..4419
                     /locus_tag="AC16CM_08190"
     CDS             4048..4419
                     /locus_tag="AC16CM_08190"
                     /inference="COORDINATES: protein motif:HMM:NF039463.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6463 family protein"
                     /protein_id="HIR3139850.1"
                     /translation="MTRLPRPTSGAALVVIGILHTSIGLLEYRAVLREMVRDGMIDTV
                     EGDAEREAAFWFLTCGVSLVSLGWSSRWARRQTGTLPSFVGLALLGIARAGVTFMPRS
                     GFWAVFVAAILAYGERHQQGG"
     gene            4423..5046
                     /locus_tag="AC16CM_08195"
     CDS             4423..5046
                     /locus_tag="AC16CM_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3139851.1"
                     /translation="MGRVYDSITGELASWLGAQPMFFVATATSDPDTRVNLSPRGLDT
                     FRVLGPDRVAWLDLTGSGVETIAHLRADGRVTLMFCAFDDPPQIIRLHGHGRVHEPGE
                     EEFGELRPRFPDLPDGRAIIDVAVERVASSCGYGVPRMELLGPRERLLDSARRKGPQK
                     LAEYRARRNAHSIDGLPGLVPDRDPVGNRRSAGRSHEPDTSAIRSRP"
     gene            5043..>5480
                     /locus_tag="AC16CM_08200"
     CDS             5043..>5480
                     /locus_tag="AC16CM_08200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3139852.1"
                     /translation="MNVLLAGASGAIGVPLTRHLISAGHTVVGLSRTPNNHDALRALG
                     AETMVVDALDRDALLRAVDGLAADALIHQLTALRNASPRLRADDPTGALRVRGTSNLL
                     DAAELVGARRFLLQSLIFGYGYGDHGTRKLTEIDPFGHAQGGYA"
BASE COUNT          956 a         1756 c         1876 g          892 t
ORIGIN      
        1 catggagatc tggtacacgg tcacgtgcgg gacctccctg agagcgagca gcccgagctc
       61 agggccgagg aaatcttgtc tatagccctc gtgccgatct tcgtcgaagg acgctggtgg
      121 gggtttatag gcttcgacga gtgcgtgcag gaacgcgagt ggtcggctgc ggaggtcggg
      181 gccctcgggg ccgccgccgg caccctgggc gcggccatca ggaggcggca gacggaggaa
      241 ctgctcaggg gaagcgagga acgctaccgg gccgttatag agcaggccac agacggcatc
      301 tacctgctcg atgtggagac ccgccgcttc ttcgagacga acccctcctt ccggcggatg
      361 ttcggctaca cggcgcaaga ggtcggggag atggacatct acgacctcgt cgcccatccg
      421 cgcgaggatg tcgacaccac catccggcaa accctgagga gcgggcgccg catcgtcggg
      481 gagcgcaagt accggcgaaa ggacggcgat ctgctcgacg tggaggtcgg ggtgagcgtg
      541 atctccctcg acgggcggga cgtggtctgc acgatcgtgc gcgacgtcac ggaacgcaag
      601 aggaacgagg aggccctgcg cggctccgag gccgagcttc gggcgctgtt cgaggcgatg
      661 acggacctca tcttcgtaat agacggggaa ggacgccacc tgaaggtcgc cctgaccaac
      721 ccggcgctgc tgtacagatc ccatgacgag acgctcgggc atacgctgca cgagctgttc
      781 ccgaaggagc aggcagacga gttcctcggc cacgttcgga gcgttctgag cacacgccgg
      841 agcgtcagct tcgagtacag cctgccgatg gaactagggg aaaagtggtt cgagggtacc
      901 gtctcgccca tgctcgagga ctcggtggtc tgggtggccc gcgacatcac ggagcgaaag
      961 aagagcgagg aagccctgac ccgcagcgag agccgcctgc gccagatcat cgagactgaa
     1021 ccggaatgcg tgaaggtgct cggcacgaac ggatcgctgc tagagatgaa ccccgccggg
     1081 ctcgccatga tcgaggccga gtccctggag caggtgcgcg gcaggtcggt ctaccgttac
     1141 attgcgccgg atcacagaga ggcgttcgtg aacctcacgg agaaggtgct gcggggcggg
     1201 tcggggacgc tggagttcga gctcgtgggg ctcaagggca cgcgccgctg gctggacacc
     1261 cacgccgtgc ccctgcgtga cgccggcggt gaggccgctg gcttgctcgc tataacgcgg
     1321 gacatcacgc agcgaaagga ggccgaaaag cggctgcggg acgcggagtt caagtacagg
     1381 acgctcgtgg agcgaatccc ggctaccatc tacatccagg taccgcggtc cgacggcgcg
     1441 agcgcctacg ccgtcacgta catgagcccc caggtggaga agctcctcgg ccacccggcg
     1501 caaaggttcg tggaggatcc gaggttctgg aacagcgtga cccatcccga cgatctccaa
     1561 cggatggtgg tcgagaccgg gcggaccgac cggacgggcg agcctttcgc cctggagtac
     1621 cgcatgctcc acgcggacgg cgattaccgg tggattcggg acgaggctgt gctcgtcagg
     1681 gacgagcagg gtaacccgat gttctggcag ggtgtcttct ccgatgtcac ggagcgcaaa
     1741 cgcgccgaag acgccatgca acagagcgaa cgtctgtacc gcacggtcat agagcaagcc
     1801 accgagaaca tcttcctcat agacgtggag agtaggcgca tcgtggagtc gaaccatgcc
     1861 ttccgggagg cgcttggtta ctccgaagag gaactgcacc gaatgacgct ctacgacgtt
     1921 gtcgccgccg accccgagag cgtggatatc aacctgcggc ggaccctgaa agagggccgc
     1981 gcctccgtcg gcgagagacg ctaccggcgc aaggacggca cgcttctgga cgtggaggcc
     2041 ggcggtagcg tcatcgttcg cgacggccgg cagacgctag tcgccgtcgc ccacgacatc
     2101 accgagcggg cgcgggccca gcacatgctg gaggaacgcg tggcgacgct ctccggtatc
     2161 gccggtgagt tgaccctgga ccggcccacg gagaccgtgc tcggcgacct cgccaggagc
     2221 gttgtcaaag ccagtacggc cgtcgcctgc ggggtggtcc tgatcggtga tggagagggc
     2281 gctgtgcatc tcttcggctc gtacggccta cccgaaaact acaccgccgc cctcggtgag
     2341 gcctaccggg ccggggtcag atcccccagc ctcgaagcgt accggagccg cgagcccgtc
     2401 ctggttcacg gcctgagggc tcgcatcctg ggcgaccctc tgtacgcccc cgtacaccgt
     2461 tacatgcgcg aggtgtcgtg ggacgtcgtg tacagtttgc ccctcgtctc acgggggagt
     2521 gcactgggtg ccatcttctt ctgtttcccc cccgctggag agcccggcga ggacgagaag
     2581 atctttctgc gggccgtcgc cgaccaggcg gccgtcgccg tcgagaacgc caggttgttc
     2641 tccgaggccc gcggcaaggc cgcactcgaa gagcgccaga ggctggcccg cgagctgcac
     2701 gactccgtgt cgcaggccct atacggcatc gcgctcgggg tcgagacggc ccgcgagctc
     2761 ctgcccgacc acccggaacg cgccgaggag cccctcgact acgccacgac cctcgccgaa
     2821 gccggcatga ccgagatgcg cgccctgatc ttcgagctga ggcccgaatc actggagaag
     2881 gagggcctgg tcgccgcact tgaaaagcaa gccgccgcag tccaggcacg ccatggcata
     2941 aaggtcgaag ccgagtggga cagggagccg gaggcgaccc tggaggtcaa agaggcactg
     3001 taccgcgtcg cccaggaagc gctgcacaac acggtcaaac acgcccgcgc gaccaacgtg
     3061 aagctgaagc tagaagagct tcccgacgtt ttcacgctcg gtatatccga cgatggtctc
     3121 ggcttcgaac cgcacaacga cttccccggt catctcggcc tcaaatctat gcgcgagcgg
     3181 gcggcgcgcc tgggtgggac gctcgaggtc acgagcgagc cgggccacgg cgtacgtatc
     3241 ctggtcagca tccccagata aagtccatgg gccgtatcag ccggttcccc tcgccgacgg
     3301 cagtggcacg ggcagagtac cgccggaggc cgcgtgatgt cacggcgcac ggcgccttcc
     3361 cgtccccctt tcgacggttc gttggatgtc gaccccggag gtaggctatg cgcacgacgg
     3421 cggcgtacgg aacggcggtg gcggtcctgg cgacggcggt caacctgctg cacgggatct
     3481 cccacgtcgg gcaggaagtt ctgtctcttc aggcgtggca atgggcgtac gtgatctgcg
     3541 tcatcttcct cgcccccctg gtcgcagcgc tcctgctatg gtcgcctttt cggctggcgg
     3601 gggcctggct cctcttcggc tcgatggtgg ggtctctggt cttcgatctc gcctaccact
     3661 tcctcatcga gggccccgat aacgtcttta cccaggagcc gggcgcgtgg cttgcggcgt
     3721 ttcgggtctc gtccgtcctg cttgtcgcca cgagcgggct cggggcgctg gtcggcggat
     3781 gggtgttgct aaggttctcg cgcccgagga cactgtctga ggcccccgtg cccgcaggta
     3841 ggggacggag atgaacgaga tgaacccgta cgcctggcgc cagcgtcgag aggacctgat
     3901 gagggagata gaacgcgaca gatcgggcag agctctgggg gcggaacgca aggcgcacac
     3961 gttctcgcca gcgtgggaac tgcggaggat ggccggcctt ctgctgaagc tcatcaggag
     4021 gttggtgtcg agaaatggag cccacccatg acccgcttac cgcggccgac ctccggcgcc
     4081 gcgctcgtgg ttattggtat cctgcatact agcattgggc tcctagagta tcgtgcggtg
     4141 ctgcgtgaga tggtgcgtga cgggatgatc gacaccgtag agggcgatgc cgagcgtgag
     4201 gcagcgttct ggtttctcac ctgcggggtc tcgctcgtct ccctgggatg gtcttcccgg
     4261 tgggcgcgtc ggcagacggg tacgctgcct tccttcgtgg ggctggcgct cctcggcatc
     4321 gcgagggcgg gggtcacctt tatgccgcgc tccgggttct gggccgtctt cgttgcggcc
     4381 atcctggcct acggcgagag acatcaacag ggaggttgag aagtgggccg agtctacgac
     4441 agcataaccg gcgagctggc aagctggctg ggggcacagc cgatgttctt cgtggcgacc
     4501 gccacctcag atcccgacac gcgcgtcaac ctctctccgc ggggcctcga caccttccgc
     4561 gtcctcgggc cagaccgcgt ggcgtggttg gatctcaccg gtagcggggt ggagaccatc
     4621 gcccacctca gggccgacgg acgtgtaacc ctaatgttct gcgccttcga cgacccgccg
     4681 cagataatac gtctccacgg ccacggtcgc gtgcacgaac ccggtgagga agagttcggg
     4741 gagctgcgtc cgaggttccc agatctgccc gacgggcgcg cgatcatcga cgtagcggtc
     4801 gagagggtcg cgagctcttg cgggtacggg gttccgcgta tggagctcct ggggccccgc
     4861 gagcgattgc tagactctgc ccggcgcaag ggaccgcaga aactggccga gtacagagcc
     4921 cgtaggaacg cgcacagcat cgacggactc cccggcctcg tgccggaccg ggacccagta
     4981 gggaaccgtc gttcggcggg aagatcgcac gagcccgata cctcagcaat ccggagcagg
     5041 ccgtgaacgt cttgctggcc ggggcttcgg gggccatcgg agtccccctt acccgacatc
     5101 tgatctccgc cggccacacg gtcgtcgggt tgagccgtac gccgaacaac cacgacgccc
     5161 tgcgcgcgct cggcgccgag acgatggtcg tagacgcttt ggaccgcgac gccttgctcc
     5221 gcgcggtcga cggcctcgcg gccgacgccc tcatccatca gctcacggcg ctcaggaacg
     5281 cgtcgccacg cctgcgcgcc gacgacccga cgggcgcgct tcgcgttcgc ggaacctcca
     5341 acctgctcga tgccgccgag ctagtgggtg cccgccgctt cctcctgcag tctttgatct
     5401 tcggctacgg atacggcgac cacgggacgc gtaagctcac cgaaattgat cctttcggcc
     5461 acgcacaggg tggctacgcc
//
LOCUS       DBNGVD010000309        12410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2986109, whole genome shotgun sequence.
ACCESSION   DBNGVD010000309 DBNGVD010000000
VERSION     DBNGVD010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2986109"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..680
                     /locus_tag="AC16CM_08205"
     CDS             72..680
                     /locus_tag="AC16CM_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139853.1"
                     /translation="MSVRVLLAEDQAMVRGAIAALLALEEDIEIVGEASRGDEVVALA
                     LESRPDVALLDVEMPGGDGLDAAASLRQELPSCRVIILTTFGRAGYLKRAMANGASGF
                     LLKDAPSSELAEAIHKVMRGERVVDPDLAIMALSAGDNPLTERERETLAASEDGATIE
                     DVAARLYLSEGTVRNYLSSAIKKLGKRNRVEAARLAQKRGWL"
     gene            complement(684..2069)
                     /locus_tag="AC16CM_08210"
     CDS             complement(684..2069)
                     /locus_tag="AC16CM_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIR3139854.1"
                     /translation="MAQAASADGSTGGNRRFVYVVASVAALGGLLFGYDTGVISGALL
                     FIKTDFQLNAFLEGFIVSSLLLGAVVGAGVSGALSDRLGRRTIILVAAVIFAIGSIGA
                     GLAPNVWVLVFFRFFLGLGVGSASALVPSYISESAPTDVRGSLSSLFQLAITLGILIA
                     YLVNAAFAPIGSWRWPVALAFVPALVLLVGMYFLPETPRWLVGKDRDEEARRVLSRTR
                     TEEEVEQELQEIRRAEEQEGEQAGYRELLAPWVRPMLVVGVGLAIFQQFVGINTVIYY
                     APTIIESTGLAEVASVLATVGIGVVNVLMTIVAIAVIDRVGRKPLLLVGLAGMTVSLV
                     ILGAAFAFSGLSGIISWVTLAGLMLYIASFAVSFGPVLWVMLPEIFPLKARGTGTGVS
                     ALSNWGANFVVAQAFLPLVAIIGRSAVFWILAGICIVAALFIQFLVPETKGRSLEQIE
                     TDLRERVGSGT"
     gene            complement(2250..4172)
                     /locus_tag="AC16CM_08215"
     CDS             complement(2250..4172)
                     /locus_tag="AC16CM_08215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3139855.1"
                     /translation="MSERNTPDRGAETPSPMAPFDAWAEWMRANTGAMTAAPGASVPW
                     LMSPGVSTGDQAEALPEGTIRTDPVLSVFEKLWDANPLQNVLPIDWIEITRSLQTLWA
                     REMSDPARAVERATDFNIKLFESTMQSWQDATNRLWGLDTQQEKEEEERPDKRFSEPD
                     WEQNPFYETLKENYLLASNFLLEEAEETDGQDTEEQRRLKFHLKQFVDAMAPVNYLLT
                     NPAALKRAYETGGVSIAEGARNLLADLQEGRLSMTDANAFQPGENLAITPGKVVYRNE
                     LIELIQYEPTTEQVYETPVMFIPPWINKYYILDLQPKNSFVKYLVDEGYTVFMISWKN
                     PDASMEDTQFEDYMTKGPLAAAEAVRDITGSDKVNPVGYCVGGTLLAITLSWLAAGDD
                     ENPFGPPTFMVALHDFTEVGETGVFIDEPSIEFMEQQMLERGYLDNRKMANMFNLLRS
                     NDLIWSNVVNNYLLGQKPPAFDLLYWNSDGTRMARDAHSFYLRNTYLENNLVQPGKVR
                     IKGRPIDLSKIEGDIYAVGAEKDHIVPWASAWKISRLADGTTRFTLANSGHIAGMINP
                     PSKGKGRYWATENGGAFETSSEWRENAKEHEGSWWEDWGKWLQDHSGEKVSPPEMGSE
                     TYPPIVEAPGTYVKEK"
     gene            complement(4391..5008)
                     /locus_tag="AC16CM_08220"
     CDS             complement(4391..5008)
                     /locus_tag="AC16CM_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139856.1"
                     /translation="MARNELRASALWARPCRAYNVQIGLDAAARASLGSIQQKLKRAE
                     PNLLVCPARTLHVSVAWLLAVHASYPAPEDSIWERHADGWTAELRRIAAESRSFRITY
                     RHLIATDSAVIALAAPTGPVNRIRRMIGERLSLPPETRNEADLVHTTLFRYNGPLSDP
                     GRFLASLETERAHATSEVDELVVSKELVFPSLQTEVLARLPLAPI"
     gene            5435..6667
                     /locus_tag="AC16CM_08225"
     CDS             5435..6667
                     /locus_tag="AC16CM_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3139857.1"
                     /translation="MAVTSEGVRRGVRPVDFVNVGKLLSDEEKSVRDRVREYVDREVI
                     PGAADYWDRAEFPFEVLPGLGELGVAGGTFSEEYGCAGWNNVAYGLALAELARGSGSL
                     ATFVHVQGGLAMAAIHELGSEEQKQRWLPAMARCEKIGCFGLTEPEAGSDPGSLGTTA
                     VERDGGYVLDGEKKWIGNASFSDVAVIWARTEDGRILGFLVETDNPGFRADVLPRKAS
                     QRAAWQTHITMKDCHVPEGARLPRAEGLGSTLSILTHSRYGVGWDGLGQAADCYETAL
                     AYAGEREQFGQPIASFQLVQQKLVGMVNEISLSQLLSIHVGRLKDAGELDPATVSMFK
                     MNNVAKARQIAAAAREVLGGNGILLDYRVMEHMADIEGVYTYEGTNDVNTLIVGQAIT
                     GHRAFSPRAPQRAEEKSG"
     gene            6840..7952
                     /locus_tag="AC16CM_08230"
     CDS             6840..7952
                     /locus_tag="AC16CM_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3139858.1"
                     /translation="MRQTSKTDPSPKHPGVMVQREQPFNAETPLELLRRDYVTPRELF
                     YVRNHGSLPAIDPATYRLTVDGLVERPLELSLEQLENEFSRVEMVATLYCAGNRRREL
                     AEVRAMPGKVPWEAGAAGNARWTGAPLHEVLRSAGVREEARHAAFTGLDEDEESGTGT
                     HFGGSVPIEKAKGDGVILAYEMNGEPLAPEHGFPLRVVVGGYIGARNVKWLSGISLQQ
                     GPSDNHYQAREYKLFPPDVTEETVDFSGGEMLGEIPLNSVVCDPGEGAVLPAGPGPVR
                     GYAVAGGARRVERVEVSCDGGESWVEASLERHGNHGTAWSFWEAGLKLAPGACQIVAR
                     ATDSADGTQPETVEEVWNFLGYANNAWHRVNVRAEG"
     gene            8038..8550
                     /locus_tag="AC16CM_08235"
     CDS             8038..8550
                     /locus_tag="AC16CM_08235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139859.1"
                     /translation="MNEGGILTLGDGTRVPVRRVRVGDAAALQRLVGRSSDRSVELRF
                     FGPLKALSDDMARRFADVDGRDKFALVALDPEYPEEIVGVVRYAREGDQDGAEYAALI
                     EDRFQGKGLGTRLTRCLIEAARENGIGYLYALVKRENADMLRLLRHLDFPERKSWQDG
                     NERVEIYLAG"
     gene            complement(8640..9851)
                     /locus_tag="AC16CM_08240"
     CDS             complement(8640..9851)
                     /locus_tag="AC16CM_08240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3139860.1"
                     /translation="MSFGNGNGNGEIIISTPLRTGIGTFGGSLKDTPATDLGATVGKE
                     VLSRAGIEGDQVDQVIVGNILSAGQGMNPGRQVGIKSGLPVEVPGMTLNRMCGSGLQA
                     IVSAAQEIALGDADVVLAGGIENMDRAPFLLDKGRYGYRMGMPSAEIQDHMVFDGLWD
                     VFNDYHMGVTAENVAEEFGITREDSDAYAVRSHQRAAMAHEEELFDGQIVPVEIKKKK
                     ETVQFTTDEHIRANASVEGLQKLKPVFKKDGGTVTAANASGINDGAALMLVSSERKAE
                     ELGLPVAGRLVSAAVAGVDPSVMGTGMIPASRLALKKAGLSVDDLDAVEANEAFASIA
                     IAVERTLEVPEEKMNPVGGAIALGHPIGATGAVLTAKILHHLARTGGQYGLVTLCIGG
                     GMGIAAIFERV"
     gene            complement(9954..10295)
                     /locus_tag="AC16CM_08245"
     CDS             complement(9954..10295)
                     /locus_tag="AC16CM_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139861.1"
                     /translation="MDERQQKQVNEAAEKFADALKESYQSIADRSVSAQELNAQVTQD
                     FFNGVINNLRTQAENNRALSEDLIEQQRKQQEASQALAQESLGAYMDFLNSMFSYYRG
                     NLDEAQRRANQ"
     gene            complement(10433..10768)
                     /locus_tag="AC16CM_08250"
     CDS             complement(10433..10768)
                     /locus_tag="AC16CM_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139862.1"
                     /translation="MPTMEQTNRAVQRLAENTRDSYQTVVDHAVGLQERNVRFARGVV
                     DGTIKELRHQAESNRSLAGELFERADEQREAYRTLVEQSLDAYMEFAYAPLAYYKENL
                     EATRKAVRR"
     gene            complement(10800..11216)
                     /locus_tag="AC16CM_08255"
     CDS             complement(10800..11216)
                     /locus_tag="AC16CM_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3139863.1"
                     /translation="MSDEAQEREVESTGRGFRGVEARPRNWLVPVILLSLREWNSYGY
                     ELMERASAFGFEAMNPGTLYRTLRQMEKEGIVESSWETSRGGPARRMYTITDAGRSYL
                     DFWAKSLEQYQRTMDNFFRLYTGRSLREERERGGEK"
     gene            11783..>12410
                     /locus_tag="AC16CM_08260"
     CDS             11783..>12410
                     /locus_tag="AC16CM_08260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HIR3139864.1"
                     /translation="MGTLNQAVVVVTGASRGLGAAIAEELASGGAKVVVNYSRSKEPA
                     DDLVEKISEAGGEAIAVQADVSDAEQAQKLIDKAIEEFNRIDVLINNAGINIDRTLKK
                     LSVEDWDKVIQVDLNSAFYTVHAVLPHMTEQGGGKIINMSSFVGEAGNIGQANYSAAK
                     AGLLGFTKTAAQELAKSGITVNAISPGFIETDMVASIPEEAQAKLKKQV"
BASE COUNT         2217 a         3877 c         3951 g         2365 t
ORIGIN      
        1 gaagcgggac ccctgccaga aggtggcttc cgcctgagcg tccatcttcc cctctcgcag
       61 gaggaaaccc gatgagcgtc cgggtgctgc tagccgagga ccaggcgatg gtacgcgggg
      121 ctatcgcagc tctgcttgct ctcgaggagg acatcgagat cgtcggagag gcgtcccgtg
      181 gcgacgaggt cgttgccctt gctctggaat cgcggcctga tgtggctttg ctcgacgtgg
      241 agatgcccgg cggcgacggt ctcgacgcgg cggcctccct gcgccaggag ctgccgtctt
      301 gccgcgtgat catccttacc accttcgggc gcgccggtta cctgaagcgt gcgatggcga
      361 acggggcctc cgggttcttg ctcaaggacg ccccatcttc ggagctggcg gaagccattc
      421 acaaggtcat gcggggtgag cgtgtggtcg accccgacct cgccatcatg gccctgagcg
      481 ctggcgacaa cccgctcacc gagcgcgaaa gggagacgct ggccgcctcc gaagacggcg
      541 ccaccataga agacgtggcg gccaggctct acctttccga aggcaccgtc cgcaactacc
      601 tctccagcgc catcaagaag cttgggaaga ggaaccgcgt cgaagcggcg cgcctggccc
      661 agaaaagagg ctggctgtaa gctctaggtg ccgctcccaa ccctctcgcg caggtcggtc
      721 tctatctgtt ccaggctgcg tcccttcgtc tctggcacca ggaactggat gaaaagtgcc
      781 gccacgatgc agatacccgc gagtatccag aaaaccgccg acctgcctat gatggccact
      841 agcggtagga acgcctgggc gaccacgaag ttggcccccc agttgctcaa ggcagagacg
      901 cccgtaccgg taccgcgagc tttgagcggg aagatctccg gcagcatcac ccagagcacc
      961 gggccgaagc taacggcgaa cgaggctatg tacagcatca gaccggccag cgtgacccag
     1021 gaaatgatgc cggagagccc ggagaacgcg aaggcggccc cgaggatcac gagcgacaca
     1081 gtcataccgg cgaggccgac gagcaacagc ggcttgcggc ccacgcggtc tatcacggcg
     1141 atggcgacta tcgtcatcag cacgttcacc accccgatac cgacggtagc gaggacggag
     1201 gccacttccg cgagccctgt agactcaatg atcgtgggcg cgtaatagat gacggtgttg
     1261 atccctacga actgctggaa gatcgcgagc ccgacaccta ccacgagcat cggcctgacc
     1321 caaggggcga gcagttcgcg gtagccagcc tgctctccct cctgttcctc tgcgcgtcgg
     1381 atctcctgta gctcctgctc tacctcttcc tcggtccggg tcctgcttag caccctacgg
     1441 gcttcttcgt cgcggtcttt accgacgagc cagcgcggcg tctccggcag gaagtacata
     1501 ccgacgagaa gcaccagcgc gggcacgaaa gcgagcgcca ccggccaccg ccagctacca
     1561 atgggagcga aggccgcgtt gaccaggtag gcgatcagga tgcccagcgt gatcgcaagc
     1621 tggaacagcg aggagagcga cccgcgcaca tccgtcggcg cggactcgga gatgtaagac
     1681 ggcaccaggg ccgaggcgct gcctacccca agccccagga agaaccggaa gaagacgagc
     1741 acccacacgt tgggcgcgag ccccgcgccg attgacccga tagcgaagat gacggcggcg
     1801 acgagtatga tcgtccgccg accaagcctg tcggagagcg ctccgctgac cccagccccg
     1861 accacagccc cgagcagcaa cgagctgacg atgaaacctt ccagaaacgc attgagttga
     1921 aagtccgtct ttatgaagag aagtgccccg gagataaccc ccgtatcgta gccgaagagc
     1981 agccctccga gagccgcgac ggaggccacc acatagacga aacgcctgtt gccacctgtc
     2041 gacccgtctg ccgacgctgc ctgggccata ctgatctcct tttcgcgctt tcttctttcc
     2101 ccatatcaga cacgtgtctg atcaccttgc cattcatgcc cagcctggcc aattaccaat
     2161 caggcaattt cagagcagcc tgcgaagacg tttaggagcg tctccgcagg cataccggaa
     2221 tacttcagac agttatcagc tcccgaagcc tacttttcct tcacgtaggt tccgggggcc
     2281 tccactatgg gggggtaggt ctcgcttccc atctcgggtg ggctgacctt ttcaccggag
     2341 tggtcttgca gccacttgcc ccagtcctcc caccaggagc cctcgtgctc cttggcgttc
     2401 tcgcgccatt cggaggaggt ttcgaaggcg ccgccgttct cggtggccca gtagcgcccc
     2461 ttgcccttgg agggggggtt gatcatgccc gctatgtggc ccgagttggc gagggtgaag
     2521 cgggtggtgc cgtcggcgag cctggagatc ttccaggcgg aggcccaggg aacaatgtgg
     2581 tccttctcgg cgcccacggc gtagatgtcg ccctcgatct tcgacaggtc tatgggacga
     2641 ccctttatcc tgaccttgcc aggctgcacc aggttgttct ccaggtaggt attgcgcagg
     2701 tagaagctgt gggcgtcacg ggccatgcgg gtgccgtcgg agttccagta caggaggtcg
     2761 aaggccggtg gcttctggcc gagcaggtag ttgttgacga cgtttgacca gatcaggtcg
     2821 ttggagcgca gcaggttgaa catgttggcc atcttgcggt tgtccaggta cccgcgctcg
     2881 agcatctgct gctccatgaa ctctatactc ggctcgtcga tgaagacccc ggtctccccc
     2941 acctcggtaa agtcgtgcag ggcgaccatg aacgtcggcg ggccgaaggg gttctcgtca
     3001 tcgccagcgg cgagccaaga gagcgtgatg gcgagcagcg ttccacccac gcagtagcct
     3061 accgggttga ccttatcgga accggttatg tccctgacgg cctcggccgc ggcgagcggt
     3121 cccttggtca tgtagtcctc gaactgggtg tcctccatcg aggcgtcggg gttcttccag
     3181 gagatcataa acaccgtgta gccctcgtcc acgaggtact tgacgaagct gttcttgggc
     3241 tgcaggtcga ggatgtagta cttgttgatc cagggcggta tgaacatgac gggcgtctcg
     3301 tagacctgct cggtggtcgg ctcgtactgg atgagctcga tgagctcgtt ccgatagacg
     3361 accttgccag gcgttatggc caggttctcg cccggctgga aggcgttggc gtcggtcatg
     3421 ctcaggcggc cttcctgaag gtcagcgagc aggttacgcg cgccctcggc gatgctgacg
     3481 ccgcctgtct catacgcccg cttcagggcc gccgggttgg tgagcaggta gttgacaggg
     3541 gccatggcat ccacgaactg cttgaggtgg aacttgaggc gccgctgctc ttctgtatcc
     3601 tggccgtctg tctcctcggc ctcctcgagc agaaagttgg aggcgagcag gtagttctcc
     3661 ttgagcgtct cgtagaacgg gttctgctcc cagtcgggct cggagaaccg cttgtcggga
     3721 cgctcctctt cctccttctc ctgctgtgta tcgaggcccc acaggcggtt ggtggcgtcc
     3781 tgccaactct gcatcgtgct ctcgaagagc ttgatgttga agtcggtggc ccgctctacg
     3841 gcgcgtgccg ggtctgacat ctcgcgcgcc cacagggtct gcagggagcg tgtgatctcg
     3901 atccagtcga tgggtaggac gttctgcagc gggttggcgt cccagagctt ctcgaagacg
     3961 gagagcacgg gatcggtacg tatggtcccc tcgggcaacg cctccgcctg gtccccggtg
     4021 ctgacgccgg gcgacatcag ccagggcacg ctggccccgg gggccgccgt catcgcaccc
     4081 gtgtttgccc gcatccactc ggcccacgcg tcgaacggtg ccatcggaga cggggtttcg
     4141 gcaccacgat ctggggtatt acgttcgctc atctgttcct ccttgagtat tttcacctgg
     4201 acggccaggc cgccggcttg tagtcggttt cactatatac ccactcttct gctaccgcta
     4261 aaagcgacgg cggatggcgg gcgattctcg aacagggtga cccgttcggc ggttgaacgc
     4321 taaaggctac aggtttcagg cttcaggtca accaactgtt gcctggtatt tgctcggcgg
     4381 gtcgatgtgt ttagatgggg gcgagcggga ggcgtgccag gacctccgtt tgcagggagg
     4441 ggaacaccag ctccttcgag acgaccagtt cgtcgacctc ggaggtggcg tgtgctctct
     4501 ccgtttcgag gctggcgagg aatcgcccgg gatcggagag cgggccgttg taacgaaaca
     4561 gcgtggtgtg caccagatcc gcctcgttgc gagtttcggg gggtaggctc aggcgctcgc
     4621 cgatcatgcg gcgaatgcgg ttcaccggcc cggtcggagc ggcgagggcg atcacggcgg
     4681 aatcggtggc tatgaggtgc ctgtaggtga tccgaaacga cctgctctct gccgcgatcc
     4741 ggcgcagctc cgctgtccaa ccgtccgcgt gacgctccca gatggaatcc tccggagccg
     4801 gataactcgc gtgcacggcg agtagccagg ccacgctcac atgaagggtc ctcgcggggc
     4861 acaccagcag gttgggttct gcccgcttta gcttctgctg gatgcttccg agcgatgccc
     4921 tggcggctgc gtccagcccg atctggacgt tgtaggcccg acagggccgc gcccatagcg
     4981 cactggctcg caactcgtta cgcgccactc tatcttggtg tgtcccacgc aattccagac
     5041 ctctctgctc gtccgaacta cgcaaacacc actctatcct cctgacgacc cacccaagag
     5101 aaaagaatga cacatcctcg aaagaaggca acaggcttca ggtttcgggc ttcaggaatg
     5161 ggaggcggat cggtcctcgg tagtcgttac acaaacgggc agtttcgggt tgttggcgat
     5221 gcgatttgtg tgagcgtgga ttcccgcttg ctcaagggaa tgacgggtgt ggggtgatct
     5281 cacagaggtg tagggtccca cgacctgtag cctgtagcct gaaaccttcc ctcctcacct
     5341 tcacgccttt cgcggggcca cgctacgttg actgggtgga ttctagaacc tatgctcaat
     5401 acggcattgg gtatcggagc gaggagtttg gggcatggct gtaacgtctg agggtgtgcg
     5461 gcggggggta cggcccgtgg atttcgtgaa cgtgggcaag ttgctctcgg acgaggagaa
     5521 gtcggtcagg gaccgggtac gcgagtacgt tgaccgcgag gtgatccccg gggccgcaga
     5581 ttactgggac agggccgagt ttccgttcga ggtgctgcca gggcttgggg agctaggcgt
     5641 cgccggcggg acgttctctg aggagtacgg gtgtgccggc tggaacaacg tcgcctacgg
     5701 gctagctctc gcggagctcg cgcgcgggtc gggctctctg gccacattcg ttcacgtgca
     5761 gggtgggctc gccatggccg ccatacacga gctggggtcc gaagagcaga agcaacgctg
     5821 gctgccggcc atggcgaggt gcgagaagat cgggtgtttt ggcctcaccg agccggaggc
     5881 gggaagcgac cccggctcgc tcggcacgac ggcggtggag agggacggcg gctacgtgct
     5941 cgacggggag aagaagtgga tcggcaacgc ctccttctcg gacgtggccg tgatctgggc
     6001 caggaccgag gatggcagga tccttggctt cctcgtcgag accgataacc ccggcttcag
     6061 agccgacgtg ctaccccgta aggcctcaca acgcgcagcc tggcagacgc acatcaccat
     6121 gaaggattgc cacgttcccg agggcgccag gttgccccgg gccgaaggcc tcggttcgac
     6181 gctctccata cttacccact cgcgctacgg cgtagggtgg gacgggctcg ggcaggccgc
     6241 cgactgctac gagacggctc tggcctacgc cggagagagg gaacaattcg gtcagcccat
     6301 agcctctttc cagctcgtcc agcagaagct cgtcgggatg gtcaacgaga tatccctgag
     6361 ccagctactc tccatccacg tgggacgcct gaaggatgca ggcgaactcg accccgcgac
     6421 ggtctcgatg ttcaagatga acaacgtcgc caaggcacgc cagattgccg cggccgccag
     6481 ggaggtcctc gggggcaacg gcatcctcct cgactaccgc gtcatggagc acatggcaga
     6541 tatcgagggc gtctacacct acgaaggcac caacgacgtc aacaccctca tcgtgggcca
     6601 ggccataacc ggccaccgcg ccttctcccc gagggcgccg caacgcgccg aagagaagtc
     6661 ggggtaatgt tgggtgagcc attagctttc agctttcagc tcttggctga ttgctgaaat
     6721 ctgacggctc tcaagcgatg atcaggacgg cccaccggcc gtgatcctcc atcgggggcg
     6781 tcaggctgca gaactcaggg atcaatgagc ggaagtctta tcagagtcag gaggcattcg
     6841 tgaggcagac atccaagaca gacccttcac ccaagcatcc cggggtgatg gtccagcggg
     6901 agcagccttt caacgccgaa accccgctgg agttgcttcg acgcgattac gtgacaccca
     6961 gggagctgtt ctacgtgcgc aaccacggct ccctaccggc gatcgaccca gcaacctatc
     7021 gcctcaccgt ggatggcctg gtggagcggc ccctggagct ctccctggag cagctagaga
     7081 acgagttctc ccgggtcgag atggtcgcca ccctctactg cgccggcaac cgccggcggg
     7141 agctcgcgga ggtccgcgcg atgccgggga aggtcccctg ggaggcgggc gccgccggca
     7201 acgcccgctg gaccggcgcc cccttgcatg aggtgctccg ctcggcgggg gtccgtgagg
     7261 aggccaggca cgcggccttc acggggcttg acgaggatga ggagtcgggc acgggcaccc
     7321 atttcggggg gtcggtcccg atcgagaagg ccaaaggcga tggggtgatc ctggcctacg
     7381 agatgaacgg cgagcccctc gcaccggagc acggcttccc gctgagggtt gtggttggcg
     7441 gctacatagg ggcccgcaac gtcaagtggc tctctgggat ctcgctccag caaggcccct
     7501 cggacaacca ctaccaggcc cgcgagtaca agctttttcc gcctgacgtt accgaggaga
     7561 cggtcgactt ctccgggggg gagatgctgg gagagatccc gctcaactcc gtggtttgcg
     7621 acccggggga aggcgcggtc ctcccggccg ggcccgggcc ggttcggggc tacgccgtag
     7681 cgggcggtgc cagacgcgta gagcgggtag aggtctcctg cgatggagga gagtcctggg
     7741 tggaggcgag cctcgaaagg cacggcaacc atggcacggc ctggagcttc tgggaggccg
     7801 gcctgaagct agcaccgggg gcctgtcaga tagtggccag ggccacggat tcggcagacg
     7861 gcacgcaacc cgagacggtc gaggaggtgt ggaactttct cggctacgcc aacaacgcct
     7921 ggcaccgcgt gaacgtgcgt gcggagggct agaagaccgg cccagcttca ccagaagcca
     7981 gagacgcggt agagttttgt gtgtgcgaca cgtgagcaca cgaagtagga acgaccgatg
     8041 aacgaaggcg ggattctgac gctgggcgac ggaaccaggg tgcccgtgcg gcgtgtcagg
     8101 gtgggagacg cagccgcttt gcagcggctc gtcggccggt cgagcgacag gtccgtggag
     8161 ctaaggttct tcggtcccct caaggcgctc tccgatgaca tggccaggcg cttcgccgac
     8221 gtggacggtc gagacaagtt cgcgctggtg gcgctggatc ccgagtatcc cgaggagata
     8281 gtcggggtcg tgcgctacgc gcgggagggc gaccaggacg gggcggaata cgcagcactc
     8341 atcgaggaca ggttccaggg caagggcctc ggtacccggt tgacgaggtg cctgatcgaa
     8401 gccgcccgcg agaacggcat agggtatctc tacgccctcg tcaagcgcga gaacgccgat
     8461 atgttgaggt tgctccgaca cctggatttc ccggagcgca agagctggca ggatggaaac
     8521 gaacgcgtgg agatatacct ggccggctag ccgatcttcc tacgagaaag ttcggctgaa
     8581 aagggcgagg cgcccggtag atgccggacg cccctgggct ggcgctctcg ctctggcgct
     8641 tagacgcgct cgaagatcgc ggctataccc atgccgccgc cgatgcacag cgtcaccagg
     8701 ccgtactggc ccccggtgcg ggcgaggtgg tgcagtatct tggcggtgag gacggcgccc
     8761 gttgccccga tggggtgtcc gagggctatc gctccgccaa ccgggttcat cttctcctct
     8821 ggtacctcga gcgtacgctc caccgcgatg gcgatagagg cgaaggcctc gttggcctcg
     8881 acggcatcga ggtcatcgac ggagagcccg gccttcttca gggccagcct ggaggcgggg
     8941 atcatacccg tccccatcac cgaggggtcg acccctgcca cggcggcgga gacgagcctg
     9001 ccggctacgg gcaagcccag ctcctcggcc ttgcgctcgc tcgagacgag catcagggcc
     9061 gcgccgtcgt tgatgccgga ggcgttggcc gccgttaccg tgccgccgtc cttcttgaag
     9121 accggcttga gcttctgcag gccctccacg ctcgcgttgg cgcggatatg ctcgtccgtg
     9181 gtgaactgta cggtctcctt cttcttcttg atctcgacgg gcacgatctg gccgtcgaag
     9241 agctcttctt cgtgcgccat ggccgcccgc tggtggcttc ttacagcgta ggcatcggag
     9301 tcctcacggg ttatgccgaa ctcctctgcc acgttctcgg ccgtgacgcc catgtgatag
     9361 tcgttgaaca cgtcccagag cccgtcgaag accatatggt cctgtatctc ggccgatggc
     9421 atgcccatcc tgtagccgta gcggcccttg tccagcagga agggtgccct gtccatattc
     9481 tcgataccgc cggccaggac cacgtcggcg tcgcccaggg ctatctcctg ggccgcggag
     9541 actatggcct gcagccccga gccgcacatc ctgttgagcg tcatgccggg aacctctacg
     9601 gggaggccgc tcttgatacc cacctgcctg cccgggttca taccctggcc ggcggagagg
     9661 atgtttccta ctatgacctg gtcgacctgg tcgccctcga tgccggcgcg cgagagtact
     9721 tccttgccga cagtggcgcc gaggtcagtg gccggcgtgt ccttgagcga accgccgaaa
     9781 gttcctattc ccgtcctgag gggcgtcgag atgatgatct cgccgttacc gttaccgttg
     9841 ccgaagctca ccctctactc cttccgtctc taagtgatca caggatacat caaaagacag
     9901 ggcccggacc acgtggccca gaccctgttc tcgccgcaaa gtgccaccca gccctactgg
     9961 ttggccctcc gctgcgcctc gtcgaggttg cccctgtagt aggagaacat ggagttgagg
    10021 aagtccatgt aagccccgag cgattcctgg gccaaggcct gggatgcttc ctgctgcttt
    10081 cgctgctgct cgatgaggtc ctcagaaagc gccctgttgt tctcggcctg cgtcctcagg
    10141 ttgttgatga cgccgttgaa gaagtcctgc gtcacctgcg cgtttagttc ctgcgccgag
    10201 acggaccggt ccgcgatgga ctggtaagac tccttgagcg cgtcggcgaa cttctcggcc
    10261 gcctcgttta cctgcttttg ctgcctctcg tccatgatct cctttcctgt tacgaaccca
    10321 cctgggtata ctttgttacc ctctgaggcc gccacgaaac acacaacggg ggagcgggcc
    10381 acccggcccg ctcccccgtc tcagaacctt cttctgcctg gcagagccct acctacctgc
    10441 ggacggcttt cctggtcgcc tccaggttct ccttgtagta ggcgaggggc gcgtaggcga
    10501 actccatgta ggcgtcgagg gactgctcga cgagggtccg gtacgcctcg cgctgctcgt
    10561 ctgcgcgctc gaacagctcg cccgcgaggc tgcggttgct ctcggcctga tgcctgagct
    10621 ccttgatggt gccgtcgacc acaccccggg cgaacctgac gttgcgctcc tgcaagccga
    10681 ccgcgtggtc cacgaccgtc tggtaagagt ccctcgtgtt ctcggcgagc ctctgcacag
    10741 ccctgttcgt ctgctccatg gtgggcattg cctctctcct ctctcgcgtg tgacgcgaac
    10801 tacttctcgc cgccgcgctc tctctcttcc cgcagcgacc tgccagtgta gagccggaag
    10861 aaattgtcca tggtccgctg gtactgctcg agcgacttgg cccagaaatc gaggtaagag
    10921 cgccccgcgt cggtgatggt gtacatcctg cgcgccgggc cgccccgcga agtctcccaa
    10981 ctggactcca cgataccctc tttctccatc tgacgtagcg tgcggtacag agttcccggg
    11041 ttcatcgcct cgaacccgaa ggccgaagcc cgctccatca actcgtaccc gtaggagttc
    11101 cactctcgta acgacagcag aattacaggc accagccagt ttcgcggcct cgcctctacc
    11161 cccctgaagc cccgtcccgt gctttctacc tcgcgctcct gtgcctcgtc actcatatct
    11221 ctctcttcct tgctctatgt gcattttaca tatatgtatc cgcacgtcaa gggtagtatc
    11281 cgaccgtatg ccggaagtcc catgatatga agagctatgg tcgactcccg gtctccgtcg
    11341 aaagtcacta ttacatctag ctcccgcgcg gctctaatcg tcctgcatat attatgacag
    11401 agcatcccgt ttgcggcttc gtgcgcttga tctgtgtcgg tggtgtcgcg gacgacggtc
    11461 cggtttccgg ccagaattgc atagaaaggt gcatgactgg tccaccctgg cgttgtagat
    11521 taggggagca tcgcgcgagg gggatcggcg tccgcaactc ctttcccgtt ctccaattta
    11581 tcaccccaca tcacgcggac gccgcccgct cgggcgcact tctttgcagc gtgcttcggg
    11641 tcacctccgc cttccagcgt gccgcttccc tcctggttcg ctttcggctt tctggtttta
    11701 ggtgctgttg gtacctagtt tgcgtgtgaa ttcttctgtc aataaacttt actccaggag
    11761 cctagtggaa aaggagggca gtatgggtac tttgaatcaa gccgttgtcg tggtcaccgg
    11821 cgccagcagg gggttaggtg cggccatcgc ggaggagttg gccagcgggg gggccaaagt
    11881 agtcgtcaac tattccagga gcaaggagcc cgccgatgat ctggtggaga agatctcgga
    11941 agccggtggg gaggccatag cggtacaggc cgatgtctcg gacgccgagc aggcccagaa
    12001 gctcatagac aaggccatag aagagttcaa ccgtatagac gtcctgatca acaacgccgg
    12061 catcaacatc gaccgtacgc tgaagaagct ctccgtggag gactgggaca aggtcattca
    12121 ggttgacctg aacagcgcct tctacacggt ccacgcggtg ctgccgcaca tgaccgagca
    12181 gggcgggggt aagatcatca acatgagctc cttcgtcgga gaggccggca acatcggcca
    12241 ggccaactac tcggcggcca aggcgggcct gctcggcttc accaagaccg cggcccagga
    12301 actggccaaa tccggcatca cggtgaacgc gatcagcccc ggcttcatcg agaccgacat
    12361 ggtcgccagc attcccgagg aggcgcaggc caagctcaag aagcaggtcc
//
LOCUS       DBNGVD010000310         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2986379, whole genome shotgun sequence.
ACCESSION   DBNGVD010000310 DBNGVD010000000
VERSION     DBNGVD010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2986379"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16CM_08265"
     CDS             complement(<1..677)
                     /locus_tag="AC16CM_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139865.1"
                     /translation="MLVEDSDDDALLILRELERAGYDVEHQRVYTAEAMEEALRSSWG
                     VIISDYRMPRFSAMEAMRMANASPSDAPFIVVSGRIGEDVAVEAMRGGAYDYVMKSNL
                     SRLPQTVERGLEKAEERRQRRKVEDELERRDAILDAVRFAADHFLGEAAGWEESIRAV
                     LGRLGEATEASRVYVFENYTGGDGELWATQRYEWVAPGISAQIDNPVLEGLPYKAAGF
                     GRWIQTLG"
     gene            complement(719..1372)
                     /locus_tag="AC16CM_08270"
     CDS             complement(719..1372)
                     /locus_tag="AC16CM_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139866.1"
                     /translation="MNVRVLIADDHGVVRQGLTMYLKLDPDLEVVGEASNGEEALQMA
                     RDLKPDVVLMDVLMPVMDGIEATEKIKAELPDVEVVALTSVLDDGAVTGAVRAGAMGY
                     LLKNTKPRELCRAIKGAAAGQVQLVPEAAARLMREMRAPEKPEALTARETEVLELLAR
                     GRANKQIARELFVEEKTVKAHVSGILRKLGVSSRTQAALYAVRTGLVSMDELNEEAW"
     gene            1710..2714
                     /locus_tag="AC16CM_08275"
     CDS             1710..2714
                     /locus_tag="AC16CM_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139867.1"
                     /translation="MSRQKPATVLDKEVSPGKPRSLPHAAYPKAADVERHGLQPLPDS
                     LPLVDFAQAEPFRSVRYSAVEAMDLTQAQLLQAAWVIAYGFARREPQARHLRPPKNPP
                     KGLMDARHTDPFGTTHFGTWDTATHMYWITRLAALTDPTTPGDAIEINEDVLEQSLVI
                     LDGEERPIGAAFNETMPPFDVEPPIREGDPFLDTVFAVWEPVYAQLGAQDAEALAALS
                     ERYPAFQKAYDEGKVTHHLLIARSDDLPGEDAFELVAASAQRCRALGYEYMVTEATNQ
                     WTGAAFEALGGVRVHFAPFQAHPVVPESDGPLENVTTSPNGFLSDKDSGGMFYVVRLA
                     "
BASE COUNT          482 a          926 c          801 g          524 t
ORIGIN      
        1 cccaacgtct gtatccagcg tccgaagccc gcggccttgt agggcaaccc ctcgagcacc
       61 gggttatcga tctgcgccga gatgcctggt gccacccatt cgtaccgctg cgtggcccaa
      121 agctcaccgt cgccgcccgt gtagttctcg aacacgtaga cgcggcttgc ctcggtcgcc
      181 tcaccgaggc gaccgagaac ggcccttatg ctctcttccc acccggctgc ctcgcccaga
      241 aagtggtctg cggcgaagcg taccgcgtcg aggatggcgt cgcgcctctc tagctcatcc
      301 tctacctttc gccgctgccg tcgctcctct gccttttcca ggccccgctc gaccgtctgc
      361 gggaggcgcg agaggttgct cttcatcacg tagtcgtagg ccccgccgcg catagcctcg
      421 acggcgacgt cctcaccgat cctgcctgac acgacgataa atggggcgtc cgagggcgag
      481 gcgttggcca ttctcatggc ctccatcgcc gaaaagcgcg gcatcctgta gtcggagatg
      541 atcacacccc acgacgaccg gagcgcctct tccatcgcct cggcggtata aacgcgctgg
      601 tgttccacgt cgtaccccgc gcgttccaac tcgcgcagga tcagcaaggc gtcatcatcg
      661 gaatcctcta cgagcaagag ccgtagatgc ttcttcagga tccctttttc cggtgtcgtt
      721 accacgcttc ttcgttcagt tcgtccatgc tgacgaggcc ggtcctgacg gcgtagaggg
      781 cggcctgggt acggctgctc acccccagct ttctaagaat gccggaaacg tgggctttga
      841 ccgtcttctc ctcgacgaag agctcccgcg cgatctgctt gttggcccgg cccctggcca
      901 acaactcgag cacctcggtc tcgcgggccg tgagcgcctc tggcttctcc ggggcgcgca
      961 tctcgcgcat taggcgtgcc gctgcctcgg ggacgagctg cacctggcct gcggccgcgc
     1021 ccttgatcgc gcggcacagt tccctgggtt tcgtgttctt gagcaggtag cccatcgccc
     1081 cggcccgcac cgcgcccgtt accgcaccgt cgtcgagcac gctcgtgagc gcaacgacct
     1141 ccacgtcggg aagctcggct ttgatcttct ccgtggcctc tataccgtcc atgacaggca
     1201 tgagcacgtc catcagcacc acgtccggtt tcaggtcgcg ggccatttgt agcgcctctt
     1261 caccgttgga ggcctctccc accacctcga ggtctggatc cagcttcagg tacatggtca
     1321 gcccctggcg gaccacaccg tggtcgtcgg cgatcaaaac cctcacgttc atgcgaatac
     1381 cgggccttcg gctcgtggtt ggtcttttgt cccagattat ctcccctgcc aaggtctttg
     1441 gcaacgatgg tgtcttggca agagcctgcg ggatcgttgg actttggtcc tacacgggtt
     1501 cgctcccgga agtctatgac gacattagtt ctttcgcccg atgccgcttc gcggggccac
     1561 cgctttacta gagagcgcaa attcgattgg caggcggtga aaccccacaa cctgtggccg
     1621 tcggtacggc ccgacgagat cagggaggtg agataaggac gattttcaga catgcaagtc
     1681 gatcggccaa cgagagaagg agtagaagca tgtcgcgaca gaagcccgca accgtactcg
     1741 acaaggaagt ttcccccggt aaaccgcgtt cactgcccca cgccgcatat ccgaaagccg
     1801 cagatgtgga gcggcacggg ttgcagccct tgcccgactc cttgccgctc gtggacttcg
     1861 cgcaggctga accctttcgt agcgtccggt actcagcggt ggaggcgatg gaccttaccc
     1921 aggcgcagct gctgcaggca gcctgggtta tagcctacgg ctttgcccga cgggaaccgc
     1981 aggcccgcca cctcaggccg ccgaagaacc cgccaaaggg gctcatggac gcgcggcaca
     2041 cggacccgtt cggaaccaca cacttcggca cctgggacac ggcgacgcat atgtactgga
     2101 tcacacgcct cgccgccctg accgacccca ctacaccagg agacgccatc gagatcaacg
     2161 aagacgtcct ggaacaatct ctcgtcatcc tggacgggga ggagcgtcct atcggcgcgg
     2221 cattcaacga gacgatgccg cccttcgacg tggaaccccc gattcgggag ggagacccct
     2281 tcctcgatac cgtgttcgct gtctgggagc cagtctacgc gcagctgggc gcccaggacg
     2341 cggaggcgct cgcggcactc tcagaacggt atccggcgtt tcagaaggca tacgacgaag
     2401 gcaaagtcac gcaccatctt ctgatcgccc gttccgacga tcttccaggg gaagatgcct
     2461 tcgagctcgt ggcggccagc gcacagagat gccgggctct cggctacgag tacatggtca
     2521 ccgaggcgac caaccagtgg actggggccg ccttcgaggc ccttggcggg gtgcgcgtac
     2581 atttcgcccc gttccaggct catcccgtcg ttccagagag cgacggaccg ctagaaaacg
     2641 tcaccacgag cccgaatggg tttctctctg acaaggacag cgggggcatg ttctacgtcg
     2701 ttcgtctcgc ctagaggcgg cgcggaccga tgg
//
LOCUS       DBNGVD010000311         7720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3350108, whole genome shotgun sequence.
ACCESSION   DBNGVD010000311 DBNGVD010000000
VERSION     DBNGVD010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3350108"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16CM_08280"
     CDS             complement(<1..700)
                     /locus_tag="AC16CM_08280"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3139868.1"
                     /translation="MSVIIDAGTRVLVQGITGQEGRFWTGHMIGLGTDVVAGVTPGKE
                     GQEVEGVPVFDTVRRATREFEADASLLFVPPRFARDAVYEALDAGIRKVVVLCEGIPV
                     HDLLRIRKAALSEGAMVIGGNTSGVISTGEAMMGFFPYWIERVYRPGKIGVMTRSGSL
                     TNEVTAQIVEAGFGASTLVGIGGDSVPLTRFAEVLPLFEADLGTDAVVIIGELGGTME
                     EEVAGAIEVGSFTKP"
     gene            complement(697..1818)
                     /locus_tag="AC16CM_08285"
     CDS             complement(697..1818)
                     /locus_tag="AC16CM_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3139869.1"
                     /translation="MKLFEYQAKNLFREAGIPVPEGRLVGEAAELEDAIREVGVPLAI
                     KAQVLQGGRGKAGLIQLASTEEEAREKGEEILQKTGRGLLVEKALDIESELYLSITPE
                     PVSGAALVMASAEGGVEIEEVAKERPERIVRELVDLSEGLLPFQARDVMFGLGLQGDA
                     MKQGAAVLQGLYDLFRRHDAELAEINPLALTEEGLVAADGKVTIDDSALWRQERFSPD
                     RDHFESDVEFDAAGEGIPYLRFDGDIGLLCAGAGLTNVIFDLVNYGGGSVNSYVEFGG
                     PNYRKGAKALELMMRSRPRIVLVVTFGTIARADVIAEDLAGAIKDLNPEFPIVAAIRG
                     TGEEKVEELLAGTGIEPLRDTEVAVEQAIELARAEGARA"
     gene            complement(1822..3276)
                     /gene="sauS"
                     /locus_tag="AC16CM_08290"
     CDS             complement(1822..3276)
                     /gene="sauS"
                     /locus_tag="AC16CM_08290"
                     /EC_number="1.2.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylating sulfoacetaldehyde dehydrogenase"
                     /protein_id="HIR3139870.1"
                     /translation="MRVRDTGDGAVAVVDEKPGPAGVVAGLVSRARAAMERVSGYDQE
                     RVNEVVQAVAWAIMERERAEELARVAVEDTGLGNVGDKIDKNQRKTLGTLRDLLEPTA
                     RSVGVVSVDEEKGITEIAKPVGVVGAVTPSTNPGATPANKVMMALKGRNAVIVAPSPK
                     GVSTCALLLEYIHAELGKIGAPLDLVQMLPEVNKEMTHELMEQADFLTVTGSASNVRA
                     GQTSGTPNACVSAGNVVSIVDRTADIEDAARKIHRSKTFDYGTSCSNDNSVVIEAPVY
                     EAMVGALEREGGYFCDEEERALLREAMWPGGGKRSPKTLCKAPAVIAEEAGLESEAAR
                     NATFFMVEGEGIGEEDPFSGEKLAVVLTVYKAPDFDEALEITKRILDYVGRGHSCGLH
                     TTDEANAERIGFEMDVCRLLINQVQVVGNGGSFDNGLNFTLSMGGGSWAGNNIGENLS
                     YKHFINITRVSRVIPEVVPTHEELLGGYWQKYGR"
     gene            complement(3475..5229)
                     /gene="xsc"
                     /locus_tag="AC16CM_08295"
     CDS             complement(3475..5229)
                     /gene="xsc"
                     /locus_tag="AC16CM_08295"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR3139871.1"
                     /translation="MTKVKMTPSEALVETLVAEGVDHVTGIVGSAFMDVLDLFPAAGI
                     RFVPVRHEQSAAHMEDAFGRVTGRAGVVIGQNGPGITNMVTSVAAANMAHTPMVVFSP
                     SAGTATIGWDGFQEADQVSIFESITKATVRVTHPSRAADALRTAFRIAYAERGPVLYD
                     IPRDYFYGEIDEEILEPHQYRADAKTAGSPASLDRAAELLAGAESPVIIAGKGVVDAE
                     AYGVVADIAEHLTAPVTNSYLHTDSFPADHPLAAGPLGYMGSKAAMKLLSEADVVLAL
                     GSRISVFGTVPQYDVDWFPRDAKIIQVDINPKQIARTHPIDVGIVADAKLAGEQILSR
                     LRDADPGRKPDEGRVSRIADEKRRWQEEIVDLAMEDGDPINPRRALLELSRVLPDDTI
                     VTTDIGNVCSTSNSYLTFKEGRKLIAALTFGNCGFAYPAALGAQLARPDCPVVAIIGD
                     GAWGMSLHEVSTAVEQNLPVIACVFNNQRWGAEQKNQVDFYDNRFVGSMIEGPNFAEV
                     ARAMGAVGIRVDDPEDVGSAFEEALRSDGPAVLEIMVDGTQLAPPFRRDALKLPERFL
                     EKYAETDHRNLARETAQA"
     gene            complement(5412..6737)
                     /locus_tag="AC16CM_08300"
     CDS             complement(5412..6737)
                     /locus_tag="AC16CM_08300"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HIR3139872.1"
                     /translation="MPRPGADRKDFAMMDGIPTRRDHESRFKLVSTPITFGAGAVEEA
                     GWELNQLGVKRALIITDPGVAATGIVEKVLESIRAQNIECGLFDRVEAEPSLRSMQEA
                     ADFAAEGGFDGFVGVGGGSSLDTAKVANLIATHPAPVADYIYPPIGEGRKPPSPLRPL
                     LAIPTTAGTGSEATTIAVLDLPELKTKAAISHRYLRPDHGIVDPLLARSMPPDVTASC
                     GLDVACHAVESFTAKPFYDRPAPQTPEERPPFQGSNPVSDIWASQALEYAGRYLGRAV
                     ADGEDIEARGHMMLGASIAGIGFGSAGTAIPHACSYPISSLKHDFRSPGYPERPFVPH
                     GFAVIVTAPAAFRFTYPASPDKHRLAAELLVGKQMHDADEDTLPNALVGLMKDAGAPS
                     GLRELGYDEDDLNALAEGALKQQRQLTIAPREAGHAELVAIFRESLDNW"
     gene            7105..>7720
                     /locus_tag="AC16CM_08305"
     CDS             7105..>7720
                     /locus_tag="AC16CM_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014644501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3139873.1"
                     /translation="MRGASPHFTHVVETTAELREVMGEPGGLALGKQLDRLDRHCCQF
                     VALSPFVLVGTSNASGAFDVSPRGDEPGFVLVLDEKTLVIPERPGNRRLDTLLNVLET
                     GSVGLLFLVPGFEETLRVNGRASVVRDADVLERTASHGKCPPVAIAVEVEECFLHCAK
                     AFKRSRLWQAEGWPERSVMPSLGRMLRDQGAAPGASTEELESGIQ"
BASE COUNT         1179 a         2652 c         2474 g         1415 t
ORIGIN      
        1 gcggcttggt aaagctcccc acctctatgg ccccggccac ctcctcctcc atcgtgccac
       61 cgagctcccc gatgatgacc acggcgtccg tccccaggtc ggcctcgaac agcggcaata
      121 cctcggcgaa acgtgttagc ggaacggagt cgccgccgat accgaccagg gtcgacgcgc
      181 cgaaaccggc ctcgactatc tgggcggtga cctcgttggt gagcgaccca ctcctggtca
      241 tgacgccgat ttttccgggg cggtagacgc gttcgatcca gtagggaaag aaccccatca
      301 ttgcctcgcc ggtggagatg acgcccgagg tgttgccccc tatgaccatc gcgccctcgg
      361 agagcgcggc cttcctgatc cgcagcaggt cgtggacggg gatgccctcg cacaacacca
      421 cgaccttccg tatgccagcg tcgagcgcct cgtacacggc gtccctggcg aaacggggcg
      481 gcacgaagag cagcgaggcg tcggcctcga actcccgcgt ggcccgcctc acggtgtcga
      541 acacgggtac gccctcaacc tcctggccct ccttgcccgg cgtcaccccg gccaccacgt
      601 ccgtgccgag cccgatcatg tgcccggtcc agaaacgtcc ttcctggccc gtgatcccct
      661 gcaccagcac cctggtcccc gcgtctatga tgacgctcat gccctcgcgc cttccgccct
      721 cgcgagctcg atggcctgct ccacagccac ctcggtgtcc cgcagcggct cgatgccggt
      781 cccggctaac agctcctcta ccttctcctc gcccgtgccc ctgatcgccg cgacgattgg
      841 aaattcgggg ttcagatcct ttatggcccc tgccaggtcc tccgctatca cgtcggcccg
      901 ggcgatggtt ccgaaggtga cgaccagcac gatcctgggc cgggaccgca tcatgagctc
      961 caacgccttg gcgcccttgc ggtagttggg gccgccgaac tccacgtagc tgttcacgct
     1021 gccgccgccg tagttgacga ggtcgaagat cacgttcgtc aggccggccc ccgcgcataa
     1081 tagcccgatg tctccgtcga atcggaggta cggtatgccc tccccggcgg catcgaactc
     1141 cacgtcgctc tcgaagtggt ccctgtcggg ggagaagcgc tcctggcgcc acagggcgct
     1201 gtcgtcgatg gttaccttgc cgtcggcggc cacgagtccc tcctccgtaa gcgcgagcgg
     1261 gttgatctcc gccagctcgg cgtcgtggcg gcggaagagg tcgtagaggc cctggagcac
     1321 ggcggcgccc tgcttcatcg cgtcgccctg gaggccgagc ccgaacatga cgtcgcgcgc
     1381 ctggaagggc agcagcccct ccgacaggtc gacgagctcc ctgacgatcc gctcaggccg
     1441 ctccttcgcg acctcctcga tctccacccc gccctccgcg ctggccatga ccagcgccgc
     1501 tcccgagacc ggctcggggg ttatggacag gtagagctcg ctctcgatgt ccagcgcttt
     1561 ctcgaccagc aagccgcgtc cggtcttctg gaggatctct tcgcccttct cccttgcctc
     1621 ctcctccgtc gaagcgagct gtatgagccc ggccttgccc cgcccaccct gaagtacctg
     1681 ggccttgatg gcgagaggaa ccccgacctc ccggatggcg tcctccagct cggcggcctc
     1741 gccgaccagc ctgccttcgg ggacgggtat gcccgcttcc cggaataggt ttttcgcctg
     1801 gtactcgaag agcttcacgc gctaccgtcc gtacttctgc cagtatccgc cgagcagctc
     1861 ttcgtgcgtc ggcacgacct cggggatgac ccgcgagacc ctggtgatgt ttatgaagtg
     1921 tttgtaggag aggttctctc cgatattgtt gccggcccag gagccgccgc ccatggagag
     1981 cgtgaagttc aaaccgttgt cgaagctacc cccgttgccg acgacctgga cctggttgat
     2041 gagcaaccgg cagacgtcca tctcgaaccc gatgcgctcc gcgttggcct cgtcggtggt
     2101 gtgcagacca caggagtgcc cgcgccccac gtagtccagg atccttttcg taatctccag
     2161 cgcctcgtcg aagtcgggcg ctttgtagac ggtgagcacc accgcgagct tctccccgga
     2221 gaaggggtct tcttccccga tgccctcccc ctcgaccatg aagaaggtgg cgttcctggc
     2281 cgcctcgctc tccaggcccg cctcttcggc gatcaccgcc ggggccttgc aaagggtctt
     2341 cgggctgcgt ttgcccccgc cgggccacat ggcctcccgc agaagcgcgc gctcctcttc
     2401 gtcgcagaag tacccgccct cgcgctccag cgccccgacc atcgcctcgt agaccggggc
     2461 ctctatcacg accgagttgt cgttggagca gctggtgccg taatcgaagg tcttgctccg
     2521 atggatcttg cgtgccgcgt cctcgatgtc ggcggtcctg tcgacgatgg agacgacgtt
     2581 gccggcgctt acgcaggcgt tcggggtgcc gctggtctgc ccggcgcgca cgttgctggc
     2641 cgagcccgtg acggtcagaa agtcggcttg ctccatcaac tcgtgcgtca tctccttgtt
     2701 gacctcgggc agcatctgca cgaggtcgag cggggccccg atcttgccca gctcggcgtg
     2761 gatgtactcc agcaacagcg cgcaggtcga gactcccttg ggggagggcg cgacgatgac
     2821 ggcgtttcgg cccttcaagg ccatcatcac cttgttggcc ggcgtcgccc ccgggttggt
     2881 ggacggcgtt accgccccga ccacgccgac gggcttggcg atctccgtga tccccttctc
     2941 ctcgtccacg cttacgaccc cgaccgacct cgccgtaggc tcgaggaggt cccgcaacgt
     3001 gcccagcgtc ttgcgctggt tcttgtcgat cttgtccccg acgttcccga gaccggtgtc
     3061 ctccaccgcc acgcgcgcca actcctcggc ccgctcccgc tccatgatgg cccacgccac
     3121 agcctggacc acctcgttca cccgctcctg gtcgtacccg gaaacacgct ccatggccgc
     3181 cctggccctc gagaccagcc ccgctactac cccggcaggg cccggcttct catccacaac
     3241 cgccaccgca ccatctccgg tatcccgaac tctcatatat tgtcaccttt acatctttgt
     3301 ctaccaggtg aatcgacaat gcgggatcgt atacggaggg gattggcctg tcaagcgggt
     3361 aggaaaggat tttgccgcaa gttttcaggt tggccggaac gccctccgga aactagcagc
     3421 cgccagccag aacgaaaagc cgacggctgc tagcttaccg ctgaaggctt cccttcaggc
     3481 ttgcgcggtc tccctggcca ggtttctgtg gtccgtctcg gcgtacttct ccaggaagcg
     3541 ctcgggcagc ttcagggcat cgcgcctgaa cggcggggcg agttgcgtgc cgtccaccat
     3601 gatctcgagc accgccggcc cgtctgatcg cagggcctct tcgaaggcgc ttccgacgtc
     3661 ttcgggatcg tccaccctga taccgaccgc gcccatggcc ctggccacct cggcgaagtt
     3721 ggggccctcg atcatggagc ccacgaagcg gttgtcgtag aagtcgacct ggttcttctg
     3781 ctcggcgccc cagcgctggt tgttgaagac gcaggcgatg accggcaggt tctgttccac
     3841 ggcggtcgag acctcgtgca ggctcatgcc ccaggcgccg tccccgatga tcgccaccac
     3901 cgggcagtcg ggccgcgcca gttgcgcccc gagggcggcg gggtaggcga agccgcagtt
     3961 gccgaacgtc agcgcggcga tcagcttgcg gccctccttg aacgtgaggt agctgttgga
     4021 ggtggagcag acgttgccga tatcggtggt aacgatggtg tcgtcgggaa ggacgcgcga
     4081 gagctccagc agcgccctgc gcgggttgat ggggtccccg tcctccatgg cgaggtccac
     4141 gatctcttcc tgccaccggc gcttctcgtc ggcgatgcgg gagacgcggc cctcgtcggg
     4201 cttgcggccc ggatccgcat ccctcagacg ggagaggatc tgctctccgg ccagcttcgc
     4261 gtcggccacg atgcccacgt cgatggggtg ggtgcgcgcg atctgcttgg ggtttatgtc
     4321 cacctggatg atcttcgcgt cccttggaaa ccagtccacg tcgtactgtg ggaccgttcc
     4381 gaagacagag atcctgctgc ccagcgcgag caccacgtcg gcctccgaga gcaacttcat
     4441 cgcggccttc gagcccatgt acccgagcgg cccggccgcg agggggtggt cggccgggaa
     4501 ggagtcggtg tgcaggtagg agttcgtcac cggcgccgtc agatgctcgg ctatgtccgc
     4561 gaccacgccg taggcctcgg cgtcgaccac gcctttgccc gcgatgatca cggggctttc
     4621 ggcgccggcg agcagctccg ccgcgcggtc cagggacgct ggggaacccg cggtcttcgc
     4681 gtccgcacgg tactggtggg gctccaggat ctcctcgtct atctccccgt agaagtagtc
     4741 gcgcggtatg tcgtagagta cggggccccg ctcggcgtag gcgattcgga aggccgtcct
     4801 gagggcgtct gcggccctgc tcgggtgggt tacgcgcacg gtggccttcg ttatggactc
     4861 gaagatggag acctgatccg cctcctggaa cccgtcccag ccgatggtgg ccgtgcccgc
     4921 ggagggcgag aacacgacca tgggcgtgtg cgccatgttg gcagccgcga cgctcgtgac
     4981 catgttggtg atgccaggcc cattctggcc gattacgacg ccggccctgc ccgtgacgcg
     5041 cccgaaggcg tcctccatgt gggccgcgct ctgctcgtgg cgtaccggca cgaagcggat
     5101 gcccgccgcc ggaaacaggt ctagcacgtc catgaacgcg gatcccacga tccccgtgac
     5161 gtgatcgacc ccctccgcca ccagcgtctc cactaaggcc tcactcggcg tcatctttac
     5221 ctttgtcacc ctggacctcc ttctgatgtc gcgctcgatg cccctgctct ggcgagcagc
     5281 actccaaccc catactactt accaccacgg acccggaaac attcatttgt tcacagtatt
     5341 ggtgcgcaat ccaagctatc agagggccgg ggaaccgtca aggaagcctg tccgggagac
     5401 agggtgctcc gttaccagtt gtccagggac tcgcggaaga tcgccacgag ctccgcgtgg
     5461 ccggcctctc tgggcgcgat ggtcagctgg cgctgttgtt tgagcgcgcc ttcggcgagg
     5521 gcgttcaggt cgtcctcgtc gtagccgagc tccctgaggc cgctcggtgc gcccgcgtct
     5581 ttcatgagcc cgaccagggc gttcgggagg gtgtcttcgt cggcgtcgtg catctgtttg
     5641 cccactagca gttcggcggc gagcctgtgc ttgtccgggc tggcgggata cgtgaacctg
     5701 aacgcggcgg gcgcggtgac gatcacggcg aacccgtggg ggacgaaagg acgttccggg
     5761 tacccgggag acctgaagtc gtgcttgagg ctagatatgg ggtaggagca ggcgtgggga
     5821 atcgccgtgc cggccgaccc gaacccgatg ccggcgatgg aggccccgag catcatgtgc
     5881 ccgcgcgcct ctatgtcctc accgtcggcc acggccctgc cgaggtacct gccggcgtac
     5941 tccagcgcct gagaggccca gatgtcggag acggggttgg agccctggaa cggcggcctc
     6001 tcctccggcg tctggggcgc cggcctgtcg tagaacggtt tggccgtgaa ggattcgacc
     6061 gcgtggcagg ccacgtccag gccgcaggag gcggtaacgt ccggcggcat ggaccgcgcg
     6121 agcagcgggt ccactatccc gtggtccggg cgcaggtaac ggtgcgagat cgccgccttc
     6181 gtcttcagct cgggcaggtc gagcacggcg attgtcgtgg cttcggagcc ggtcccggcc
     6241 gtcgtcggta tcgccagcag gggccgaagt ggggaagggg gcttgcggcc ctcgccgatg
     6301 ggaggataga tgtagtccgc cacgggcgcg gggtgggtgg cgatcaggtt cgccaccttg
     6361 gccgtatcga ggctcgaccc gccaccgacg ccaacgaaac cgtcgaaccc accttcggcg
     6421 gcgaagtccg cggcttcttg catggatcgc agcgaaggct cggcctcgac ccggtcgaac
     6481 agcccgcact cgatgttctg ggcccggatc gactccagca ccttctctac gatgccggtc
     6541 gcggccacgc cggggtccgt gatgatcagc gcccgtttga cgccgagctg gttcagctcc
     6601 catcccgcct cctcgacggc cccggctccg aacgtaatgg gcgtcgaaac gagcttgaaa
     6661 cggctctcgt ggtcccgcct cgtcggtata ccgtccatca tggcaaagtc cttccggtcg
     6721 gcgcctggtc ggggcaaagg cctcctcgcg tgccggaggc tctggccggg gatcatattt
     6781 tcggggccac gtcgtgtcaa tcgcacgccc ctcgatcccc atacgtctca tcgtccgaaa
     6841 cgccaccaca aaaacagccg gcctccgcgc cgctcgtcgg gaatagtttc ttctccccag
     6901 gcaacagcgt cgaggatacc gtctatcttc cggctgacgg cctgggtgcc cgggcccgaa
     6961 aaatcgtcgc cgcgtcgact aacatgtgtg ggtcttgcgc gagacggggc atggcgagca
     7021 cggatggccg tgtcggggcc ctccttgcgg cgcgaggaac cacggcgcag gacgaagtaa
     7081 aggttgccat cgaggaggag cgtcatgcga ggagctagcc cacacttcac ccacgtggtc
     7141 gagactacgg cggagctgcg cgaggtcatg ggggagccgg gcgggctggc gctcgggaag
     7201 cagctcgatc gtctggaccg ccattgctgc caattcgtgg ctctctcgcc gttcgtgctg
     7261 gtcggtacct ccaacgccag cggggcattc gacgtatccc cgcgtggtga cgagccaggg
     7321 ttcgtgctcg tgctggacga gaagacgctc gtgatccccg agcgtccggg caaccgcagg
     7381 ctagatacgc tcctgaacgt cctggagacc ggctcggtcg ggttgctctt tctcgtaccg
     7441 ggtttcgagg aaacgctcag ggtcaacggc cgcgcgagcg tggtacggga cgcagacgtt
     7501 ctggaacgga ccgcctccca cggaaagtgc ccgccggtgg ccatagcggt ggaggtcgag
     7561 gagtgtttcc tgcactgcgc caaggccttc aagcgctcgc gtctctggca ggcagaaggg
     7621 tggccggagc gttcggtgat gccgtcgtta gggcggatgc tccgcgacca gggggccgca
     7681 ccgggcgcga gcacggaaga actggagagc ggcatccagg
//
LOCUS       DBNGVD010000312         6361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3168649, whole genome shotgun sequence.
ACCESSION   DBNGVD010000312 DBNGVD010000000
VERSION     DBNGVD010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3168649"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="AC16CM_08310"
     CDS             complement(<1..957)
                     /locus_tag="AC16CM_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3139874.1"
                     /translation="MPRRDDIASILIIGSGPIVIGQAAEFDYSGTQACRALRDEGYRV
                     VLVNSNPATIMTDPEVADATYVEPLTAETVAEILRHERPDALLPTLGGQTALNLAIEL
                     HEAGVLDELDVELLGASITSIRKAEDRQLFSEAMQRIGQKVPASRTVKSLAEAEELAG
                     EIGYPLIIRPSFTLGGKGGATANDHSELRRAVADGLDASPVGSALVEKSVAGWKEFEL
                     EVMRDLQDNVVVICSIENVDPMGVHTGDSITVAPAQTLSDRQYQTLRSASLRIIREIG
                     VSTGGSNIQFAVDPESDEHYVIEMNPRVSRSSALASKATGFPI"
     gene            complement(959..2077)
                     /gene="carA"
                     /locus_tag="AC16CM_08315"
     CDS             complement(959..2077)
                     /gene="carA"
                     /locus_tag="AC16CM_08315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3139875.1"
                     /translation="MEYVRSQGRSKATLVLEDGAAFEGWTFAGEGEVAGEVVFTTSMV
                     GYQETITDPSYRGQILLFTYPLIGNYGVIPGDDESRRVQAAAVLVREYTPHPSNWASE
                     RSLADLLEQENVLGIEGVDTRALTRHLRDRGAMRGVISTSESDRNVLRDRATAHPSMV
                     GLDLASTSSELTEPTLLPAFGEERCRVVALDYGVKGSIYRELRSHGASVLAMPGSTNP
                     DDILATGPDGLFLSNGPGDPAALGDAVNTLRSLLGEVPTFGICLGHQLLGLALGCETY
                     KMPFGHHGANHPVRNLATGRIEITSQNHGFAVNEDTLQDGVELTHRNLYDGTVEGIAS
                     RDLRAWSVQYHPESSPGPRDSGYLFEDFVEGISSKARA"
     gene            complement(2061..3368)
                     /locus_tag="AC16CM_08320"
     CDS             complement(2061..3368)
                     /locus_tag="AC16CM_08320"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3139876.1"
                     /translation="MTPDKSPAELLIRGAHVLDPSSGLDGITDVRISSGRISEIGEGL
                     RGTREIDAAGLHLFPGFVDVHAHWRTPGREDEETIETGSAAAAAGGFTGVVMMPNTDP
                     IVDRPVVVSGLVRRVERESRVRARVSAALHVGLAGERLTEMRLLKEAGAFCVSDDGLG
                     TASAGVLRNGMLYACSAGLPVILHCEDHTLATGVVHDGVAASLAGLPGSPASAEDTAT
                     ATALILAAETGAKVHITHVSTTLSAALVGFFKNRADVTSDTTPHHLTLTDELVSTLEG
                     LYRVNPPLRPDGDRGGVEAALRDGVLDFVATDHAPHAQEEKELPMEEAAPGFLGHETA
                     FAALYTDLVLGGRIPLTRLVEAMSCAPGRWVGEGGTLGVGQPADLTLVDLSEEWVVDR
                     GAMVSRSSNSPYLGRTLTGRILSTIVGGEPVHDRTGAEFGVRA"
     gene            complement(3365..4264)
                     /locus_tag="AC16CM_08325"
     CDS             complement(3365..4264)
                     /locus_tag="AC16CM_08325"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3139877.1"
                     /translation="MSLQDTSREELREVLDLATAFANGRITDALAHKTICLAFFEAST
                     RTAVSFELAARRSGAHVISLSERGSAISKGESLIDTVVTLDSLGADAIVLRHPSVGAA
                     ALATRHTSAPVINAGDGCGQHPTQALLDLYALSDALGGFDELAGRRVAIVGDVLHSRV
                     ARSVIPAFVSSGLEVALVAPRTLLPPEAEAWGLPMLSSVDEAVGWGADVLYMLRLQKE
                     RMTGARIPSIAEYARYYGVARHHLHDGLLIMHPGPVNRGVEISGECVLDPSSLIPDQV
                     AAGVCVRSAVLALSTGAIQRAAA"
     gene            complement(4272..4826)
                     /gene="pyrR"
                     /locus_tag="AC16CM_08330"
     CDS             complement(4272..4826)
                     /gene="pyrR"
                     /locus_tag="AC16CM_08330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3139878.1"
                     /translation="MGRSYVYESAHSQVLSPNDLGRSLRRISHEILERNASSLDSLAL
                     VGVLTRGVPLAGRISANIRQFEGIDVPVGSLDITLHRDDLEQAGPEVRGSSVPFDVSG
                     RTVVLVDDVLYTGRTARAAMDALLELGRPAAVRLAILVDRGHRELPIRADHVGKNVPT
                     ARGQRVLVNLEETDGEDGVIVVGD"
     gene            complement(4979..6319)
                     /locus_tag="AC16CM_08335"
     CDS             complement(4979..6319)
                     /locus_tag="AC16CM_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139879.1"
                     /translation="MLFGAAASGEGAATRWVVVLCWVAVAMDGFDLVVLGAVAPALLE
                     YREWGLTAPQVGAITSYGLFGMLIGALGIGTLSDVIGRKRSILVSVAGFSVFTALCAV
                     APTPGIFGMLRFLAGLGLGGLIPTAATMVSEYAHVRRGGSSITFMMTGYHVGGVLTAL
                     LAIPVLPSLGWRAMFVIGALPALVIVPLIIRNLPESTSFLIARGRRVEAEELASRYGI
                     ALEAEEVREVETEERASEGAGFRALKTLFSGGYLLATLAFWVASFMGLLLVYGLNQWL
                     PEIMRQSGYALGAALTFLLVLNVGAVIGLLVAGPVSDRYGSKSACAGWFALAAVFLFL
                     LSIQMPLIATYVVVLLTGFFVFSAQVLLYAYVSKHYPTSGRGTALGWAAGIGRVGAIC
                     GPILGGMLLGAGLAVPWGFYAFACVGLLGCIFIAAVPRSPAEALRQTSPEGATQ"
BASE COUNT         1100 a         2117 c         2108 g         1036 t
ORIGIN      
        1 gattgggaac cccgttgcct tgctcgccaa cgcactcgac cggctcaccc tggggttcat
       61 ctcgatcacg tagtgctcat cactctccgg gtcgaccgca aactggatgt tcgatccacc
      121 cgtggagacc ccaatctcac gtattatgcg taaagacgcg gagcggaggg tctggtactg
      181 gcggtcggag agggtctggg ccggggccac ggttatggag tcgccggtat ggacgcccat
      241 ggggtcgacg ttctctatgg agcagatcac gacgacgttg tcttggaggt cgcgcatgac
      301 ctcgagctcg aactccttcc acccggcaac gctcttctcg acgagggccg accctacggg
      361 gctcgcgtcg aggccgtctg cgacggcgcg gcggagctcg gagtggtcgt tggccgtggc
      421 ccctcccttg ccccccaggg tgaagctggg acggataatg agggggtagc ctatctcccc
      481 ggccagttct tccgcctcgg cgaggctctt cacggtacgg ctggcgggga ccttctggcc
      541 gatgcgttgc atggcctccg agaacaactg gcggtcttcg gccttgcgga tcgaggttat
      601 cgaggcgccg aggagctcta cgtcaagctc gtctaggacg cctgcttcat gcaactctat
      661 ggccaggttg agggccgtct ggccgccgag cgtgggaagc agggcgtcag gccgttcgtg
      721 gcgcaggatc tcggcgaccg tctcggcggt gaggggctct acgtaggtgg cgtcggcgac
      781 ctcggggtcg gtcattatgg tcgccgggtt ggagttgacg aggaccacgc ggtacccctc
      841 gtcgcggagg gcgcggcagg cctgggtgcc ggagtagtcg aactcggcgg cctgacctat
      901 aacgatgggt ccagacccga tgatgaggat gcttgcgatg tcgtcgcgac gcggcacgct
      961 aggccctcgc cttcgacgag atgccctcca cgaaatcctc gaagaggtac cccgagtccc
     1021 tcgggccggg gctcgactcg ggatggtact gcacgctcca ggcacgcaaa tccctgctgg
     1081 cgatgccctc gacggtgcca tcgtagaggt tgcggtgggt cagctcgacg ccgtcctgca
     1141 aggtatcctc gttaaccgcg aaaccatggt tctggctcgt tatctctatg cgccccgtcg
     1201 cgaggttcct tactgggtgg ttcgcgccgt ggtgtccgaa gggcatcttg tacgtctcgc
     1261 aaccaagggc gaggccgaga agctggtgcc ccaggcagat gccgaacgtc ggtacctcgc
     1321 caagcagcga acgcagggtg ttcacggcat cgccaagggc cgccgggtcg ccagggccgt
     1381 tggagaggaa caggccgtcc ggcccggtgg ccaagatgtc gtccgggttc gtcgagcccg
     1441 gcatggcgag aactgaggcg ccgtggctcc tgagctccct gtagatggag cccttcaccc
     1501 cgtagtctag ggccacaacg cggcaacgct cctccccgaa ggccgggagc agggtcggct
     1561 ccgtcagctc cgagctggtg gaggcgagat ccaggcccac catggaagga tgggcggtgg
     1621 cccggtcgcg tagaacgttg cggtccgatt ccgaggtcga gatcacaccc cgcatcgcgc
     1681 ccctgtctct gaggtgacgc gtgagggcgc gcgtgtccac accttcgatg ccgagcacgt
     1741 tttcttgctc cagcagatcg gccagggagc gttcgctggc ccagttgctc gggtgggggg
     1801 tgtactcgcg gaccagcacg gcggctgcct gcaccctgcg tgactcgtcg tcgccgggga
     1861 tcacgccgta gttgccgatc agggggtagg tgaagagcag gatctggccc cggtaggagg
     1921 gatcggtgat ggtctcctgg tagccgacca tgctcgtggt gaagaccacc tcgccggcta
     1981 cctcgccttc tccagcgaag gtccagcctt cgaacgcggc cccgtcttcc agaaccagcg
     2041 tcgccttgct cctaccctgg ctacgcacgt actccaaact ctgctcccgt cctgtcgtgt
     2101 accggctcgc cgccaactat agtgctcaag atcctgcccg tcagggtcct tccaagatat
     2161 ggcgagttgg aagaacggct gaccatggcg ccccggtcta caacccactc ctcggagagg
     2221 tccacgagcg tcaggtccgc aggctggcca accccgagcg taccaccttc gccaacccac
     2281 cggccagggg cgcagctcat ggcttcgact aggcgcgtca gcgggatcct gccgccaagg
     2341 accaggtcgg tatagagcgc ggcgaaggcc gtctcgtgtc cgagaaagcc cggcgcggcc
     2401 tcctccatcg gtagctcctt ctcctcctgg gcgtgcggcg cgtggtccgt ggccacgaaa
     2461 tcgaggacgc cgtcacgcag ggcagcctcc acgccaccgc ggtccccgtc tggcctgagc
     2521 ggggggttga ccctgtagag cccctccagg gtcgagacga gctcgtccgt cagcgtgagg
     2581 tgatggggcg tggtgtccga agttacgtcc gccctgttct tgaagaagcc gaccagcgcc
     2641 gccgacagcg tcgtagagac gtgggttata tggaccttcg cccccgtctc agcggccagg
     2701 atcagggccg ttgcggtggc cgtgtcctcc gcgctcgccg gggatccggg gagtccggcg
     2761 agtgaggcgg cgacgccgtc gtgaacgacg ccggtggcga gcgtgtggtc ctcgcagtgc
     2821 aggatcaccg gtagcccggc cgagcaggcg tagagcatgc cgttcctgag aaccccggcc
     2881 gacgcagtcc cgagcccgtc atcggagacg cagaaggcgc cagcctcctt aagcaggcgc
     2941 atctccgtga ggcgctcgcc agcgagcccg acgtggagcg ccgctgagac gcgggccctg
     3001 accctggatt ctctctccac gcggcgtacg aggccgctaa ccacgacagg cctgtctacg
     3061 atggggtcgg tgtttggcat catcacgacg ccggtgaagc cgcccgccgc cgccgccgcc
     3121 gagccggtct ctatggtctc ctcatcctcg cggcccggcg tgcgccagtg ggcgtgcacg
     3181 tctacgaagc cgggaaagag gtgaaggccg gcggcgtcga tctcccgcgt gccgcgcaga
     3241 ccctcgccta tctccgagat cctgcccgac gagatgcgca catctgtgat gccgtcgagg
     3301 cccgaggacg ggtcgagcac gtgggctccg cggatcagca gttccgctgg tgacttatcg
     3361 ggggtcacgc ggcggcccgc tggatggcgc cggtcgagag ggcaaggacg gcggagcgta
     3421 cacacacgcc ggcggcgacc tggtcgggga tcagggacga agggtcgagg acacactcgc
     3481 cggagatctc gacgcccctg ttgacggggc ccggatgcat gatgagaagc ccgtcgtgca
     3541 ggtgatgccg cgccacgccg taatatctgg cgtactcggc gatggagggg attcgggcgc
     3601 ccgtcatccg ctccttctgc aggcgcagca tgtagaggac gtcggccccc caccctacag
     3661 cctcgtccac cgaggagagc atgggtagtc cccaggcctc ggcttcgggt ggcagcagcg
     3721 tgcggggggc gacgagggcc acttccagcc cggagcttac gaacgccggt atcacgctgc
     3781 gggccacgcg gctgtgcagg acgtccccga cgatggcgac gcggcgtccg gcgagttcgt
     3841 cgaaaccgcc gagcgcgtcg gagagtgcgt agaggtcgag gagggcctgg gtcgggtgct
     3901 ggccgcagcc gtctccggcg ttgatcacgg gcgccgaggt gtgcctggtg gcgagtgcgg
     3961 ctgcgccgac ggatgggtga cgcagcacta tggcgtcggc gccgaggctg tccagcgtga
     4021 cgacagtgtc gatcagggac tcgcccttgg atatggccga gcccctttcg gagagcgaga
     4081 tcacgtgcgc cccagaacgc ctcgcggcta gctcgaacga gaccgccgtc ctggtcgagg
     4141 cctcgaagaa ggccaggcag atggtcttgt gcgcaagcgc gtccgtgatg cgcccattgg
     4201 cgaacgcggt ggcgaggtcc aggacctcgc gcagttcttc gcgactcgtg tcttgcaggc
     4261 tcaaaaaact cctagtctcc aacgacgatc accccgtcct ccccatcggt ctcctccagg
     4321 ttgaccagaa ccctctgacc gcgcgcggtc ggcacgttct tgcccacgtg gtcggccctg
     4381 atcggtaact ctctgtgtcc ccggtcgacc aggatggcca accggaccgc ggcaggacgt
     4441 cccagctcca gcagggcgtc catcgccgcc cgcgccgtcc tgcccgtata caggacgtca
     4501 tccacgagta cgaccgtcct gccgctcacg tcgaagggca cgctgctgcc ccgcacttcg
     4561 ggaccagcct gttcgagatc gtcacggtgg agcgttatat cgagtgaacc aaccgggacg
     4621 tcgatgccct cgaactggcg gatgttggct gagatcctcc ccgccagtgg gacaccccgg
     4681 gtgagcaccc cgaccagcgc gaggctatcc aggctcgaag cgttccgttc caatatttcg
     4741 tgggagatgc ggcgtagcga acggccgagg tcgttcgggg atagtacctg cgagtgggcg
     4801 ctctcgtaca cataggatcg gcccaagccc tgcctccttc gcgtctcaca ggacgccgtt
     4861 aaaggatcgg tctgccgagc gtcaagctac cagagggccg ggggggcgtc aaggggtgga
     4921 aagctctcag ctctcagcca tcagctgtca gcaagaaccc atcgctgaac gctcttcgtc
     4981 actgcgtcgc gccttccggc gatgtctgcc ttagggcctc ggccggggag cggggtacgg
     5041 cggcgatgaa gatgcagcct agcaacccga cgcaagcgaa ggcgtagaag ccccacggta
     5101 cggccagccc cgcccccagg agcatcccgc ccaggattgg gccgcagatc gccccgaccc
     5161 tgcctatgcc cgccgcccat ccaagggccg tgccgcgccc gctcgtaggg taatgcttgc
     5221 tcacgtaagc gtagagcaga acctgcgcgc tgaacacgaa gaagcccgtg agaaggacca
     5281 cgacgtacgt cgcaatcagt ggcatctgaa tgctaaggag gaacaggaag accgcggcaa
     5341 gcgcgaacca ccccgcgcag gcggacttcg agccgtatcg gtccgacact ggcccggcaa
     5401 cgaggagccc gatcacggcc cccacgttga gcaccagcag aaacgttagg gccgcaccaa
     5461 gggcgtatcc cgattgccgc atgatctccg gcagccactg gttgaggccg tacacgagca
     5521 ggagccccat gaaagacgca acccagaacg cgagcgtcgc cagaagatac ccgccggaga
     5581 acaacgtctt gagtgccctg aaaccagccc cttcggatgc cctttcctcg gtctcgacct
     5641 cgcgcacctc ctccgcttcg agcgcgatcc cgtagcggga agccagctcc tccgcctcca
     5701 ccctccggcc ccgtgcgatc agaaaactcg tggattcggg gaggttgcga atgatcaggg
     5761 gcacgatcac gagtgccggc agggccccga tgacgaacat ggcgcgccag ccgagggagg
     5821 gcagcaccgg gatggcgagc aacgccgtga gcaccccgcc gacgtggtag cccgtcatca
     5881 tgaaagttat ggaggatccg ccgcgccgca cgtgggcata ctcggagacc atcgtggcgg
     5941 cggttgggat caggccgccg agcccgagcc ccgcgaggaa gcggagcatc ccgaagatgc
     6001 caggcgtcgg ggcaacggcg caaagggccg taaagaccga gaagccggcc acgctaacca
     6061 ggatggaccg cttgcgcccg atcacgtccg agagcgtacc tatgccgagc gccccgatca
     6121 gcatcccgaa caacccgtat gaggttatgg ctcccacctg cggggccgta agcccccact
     6181 cccggtactc cagaagcgcc ggggccaccg ccccaagcac cacgagatcg aagccgtcca
     6241 tcgccacagc aacccagcac aagaccacaa cccaacgcgt cgccgcacct tccccactgg
     6301 ccgcagcccc gaacaatatc ggctcctttc tcctctttgc ccggatattc tgaccataat
     6361 c
//
LOCUS       DBNGVD010000313         5270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_617387, whole genome shotgun sequence.
ACCESSION   DBNGVD010000313 DBNGVD010000000
VERSION     DBNGVD010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_617387"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC16CM_08340"
     CDS             <1..322
                     /locus_tag="AC16CM_08340"
                     /inference="COORDINATES: protein motif:HMM:NF016114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR3139880.1"
                     /translation="LEYIREQGGEGDICGAYYAFDGSPVHLEMNERSIAISAEQLSSV
                     PNRVGVSSGPEKPRANIGAARSRFLNVLITDEHTAGQMLEILDEESLQPSEVSQHKKP
                     NARS"
     gene            complement(356..832)
                     /locus_tag="AC16CM_08345"
     CDS             complement(356..832)
                     /locus_tag="AC16CM_08345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HIR3139881.1"
                     /translation="MDGKRYRMAVGSDDAGVNLKNFIRDQLGEDERVEEVIDFGVADA
                     DDKTPYPVVCIKAAEAVARGEADRAVLFGGTGLGEAISANKVQGIRAVVAHDPYSIER
                     SILSNNCQVLCLGERVIAPELADRIVKQWLGYEFDTSSPSAAKVQAITDYEENGSL"
     gene            993..1511
                     /locus_tag="AC16CM_08350"
     CDS             993..1511
                     /locus_tag="AC16CM_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139882.1"
                     /translation="MRQEIEVGEYRFEDDGTIPNNPTLPLLVYPQALNGSDLDPSRCK
                     ALLFENGWGGSWVDGVFPYHHYHSTSHEVLCVVGGSASIAFGGPQGETIEVSAGDVVV
                     IPAGVGHCKRGSDGGFSVVGAYPKGQENYDLCTGEEGERPGVLENIENAALPESDPLF
                     GEEGPLVRRWKG"
     gene            1972..2202
                     /locus_tag="AC16CM_08355"
     CDS             1972..2202
                     /locus_tag="AC16CM_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139883.1"
                     /translation="MMRALLCNCRQHLEAENDAALCAVVREHLIAMHPDIEPSREQVE
                     EIVATRAYDLEYKEVYPDVDFAFGPVSRGPRE"
     gene            2207..2482
                     /locus_tag="AC16CM_08360"
     CDS             2207..2482
                     /locus_tag="AC16CM_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139884.1"
                     /translation="MEPENMLAGATVRVRERHRIAERRGMVGRVTGRYGGEGYVALDV
                     RFSGGRQRLFRPQDLEEISPPQPWWRSLLNLERTRNRTDSRLRTLDS"
     gene            complement(2687..4777)
                     /locus_tag="AC16CM_08365"
     CDS             complement(2687..4777)
                     /locus_tag="AC16CM_08365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3139885.1"
                     /translation="MSTTERATGRAIGMPLDRIDGPQKVKGTAPYAYEHHVEHPLYLF
                     AVRSSIARGRIVSMDVSRAQDVPGVVIVVTHENAPRMADTRDQELATLQSDRVDYRGQ
                     YVGAVIAETYEVARHAASLVEISYEEETHAAVLDPASDGLYAPEVVNAGFPTDTEEGD
                     FDRSFSSAAVRLDETYTTPTEHNNPMEPHTTVAIWEGGNLTLYESTQGVHPVGRTVAR
                     VFGLDRDRVRVVAPYVGGGFGSKGLPHANTMLAVLAAQMVEGRPIKFALTRQQMFAGV
                     GYRTPTVQRMRLAADEEGHLTAVGHDVIEQTARFKEFAEQTAVVSRMMYATQSRRTTH
                     RLAALDVPVPSWMRAPGEAPGMFALESAMDELAEACNLDPIEFRVRNEPEIDPESGLP
                     YSSRNLVACLREGARRFGWEDRDPAPRTRQEGRWLVGTGVASATYPVFRMPGSRATIR
                     YEGDGRYAVEIGAADIGTGTWTALTQISADALEIGVEDVDLRIGDTRLPYASVEGGSS
                     GITAWGAAIFEAARVFRERHGEDPSEGDEVQARTPNNPETENYAMHAFGAYFVETRVD
                     ADTGEVRVPRMTGVFAVGNVINPGTARSQFIGGMAMGLSMALHEESLVDGRFGHVVNQ
                     DLAEYHIPTNADVGEIDVSWIDEDDPYVNPMGSKGIGEIGIVGSAAAIANAVYHATGI
                     RVRDLPITPDKILP"
     gene            complement(4774..>5270)
                     /locus_tag="AC16CM_08370"
     CDS             complement(4774..>5270)
                     /locus_tag="AC16CM_08370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018851989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3139886.1"
                     /translation="MAVAMAALDAFVNVRGPEGGRRIPIEDLHRLPGDEPRHDTVLEH
                     GELITSVDLPPPGFLSNSKYRKVRERASYAFALVSVAAALDVQDGVVRDVRIALGGVA
                     HKPWRATKAEEALRGEPAGEENFRAAADIELESAEPLQDNAFKVPLARNVITRTLLEL
                     SEGR"
BASE COUNT          853 a         1716 c         1740 g          961 t
ORIGIN      
        1 tctggagtac atcagggagc agggtggcga gggcgacatc tgcggcgcct actacgcctt
       61 cgacggctcg cccgtccacc tggagatgaa cgagcgctcc atagccataa gcgcggagca
      121 actctcgagc gtacccaacc gcgtcggggt cagctcgggc cctgagaaac cgcgggccaa
      181 catcggggcg gcccgctccc gcttcctgaa cgtcctgatc acggacgagc acaccgcagg
      241 ccagatgctc gagatcctcg acgaggagag ccttcagcca tcggaggtca gccagcacaa
      301 gaagccaaac gctagaagct gatagctgaa ggcgcggtgc gcaaaacgaa ccgctctaga
      361 ggctgccgtt ctcctcgtag tcggttatgg cctgaacctt ggcggccgat ggggacgagg
      421 tgtcgaactc gtagcccagc cactgcttca cgatgcggtc ggccagttcg ggggcgatca
      481 cacgttcccc gaggcagagc acctggcagt tgttcgatag tatggaccgc tctatggaat
      541 aggggtcgtg ggcgactacg gcgcggatgc cctgcacctt gttggccgag attgcctcgc
      601 ccaggccggt acccccgaag agcacggccc tgtcggcctc gcccctggca accgcctctg
      661 cggcctttat gcagactacc gggtagggtg ttttgtcgtc ggcgtcggcc acaccgaagt
      721 cgatgacctc ctcgacccgc tcgtcttccc cgagctggtc cctgatgaag ttcttgaggt
      781 tgaccccggc atcgtcggag ccgaccgcca tcctgtaccg cttgccatcc atctcgctgc
      841 gctcccttcg accggtgtct tgctggcgtc gtctctcgcg ggctacttac cccgctcgtg
      901 ccacggtcaa aatggggcgg ctggttgaag cccacgacac cggggaacat cgagattgcg
      961 aacgtctccg tggcggccac cggaaggaag agatgaggca agagatcgag gtcggagagt
     1021 accggttcga ggatgacggc accatcccca acaacccgac gctaccgttg ctcgtgtacc
     1081 cgcaggcgct gaacgggtcg gacctggatc cctcccgatg caaggcgctg ctcttcgaga
     1141 acggctgggg aggctcctgg gtggacggcg tcttccccta ccaccactac cactcaacct
     1201 cccacgaggt cctatgcgtc gtcggcggat cggcgagcat agccttcggc ggcccgcagg
     1261 gtgagaccat cgaggtctcg gccggcgacg tggtcgtgat accggccggc gtggggcact
     1321 gtaaaagggg ctctgacggc gggttctccg tggtcggggc ctaccctaaa ggtcaggaga
     1381 actacgacct ctgtaccgga gaagagggcg agcgtcccgg ggtactggag aacatcgaga
     1441 acgccgcact gccggaatct gatcccctgt tcggcgaaga aggaccgctc gtgagacgtt
     1501 ggaaagggtg accttcgcga agtatcctct gcgatccgcc ggagcgcttc cgcggccctg
     1561 atcacgacgt gccggtccgc gtgccagacg gcccccatct ccctcagctc tatcatagcc
     1621 ctggtgacgg cgatggtaga ggcgtacccg gcctccgagc ggcaagaata tcgtgcccct
     1681 gcaagcgggc gtaaacacgt gctcgcttcc cctggaatct ctagaaggta tgccctaggc
     1741 taaccgtccg agtctctccg gcgagagtgg cgagaagaga ccgacttcgg aaaccaacgt
     1801 cgtgtgctct gcatccagtt tctggtctta ctatcgtccg gccgaatact ccctgcccac
     1861 ggctcttgta tcccacgata ccgacactgt gaccgtcatt ggtcaagatg cgtgctgcaa
     1921 ggttcttgcc gtcctcgtga gggtggcaag ttcttcggga aaggagaaga tatgatgcgc
     1981 gcattgcttt gcaattgccg ccagcatctc gaagcggaaa acgacgccgc gctatgcgcc
     2041 gtggtacggg aacaccttat cgccatgcac cctgacatcg agccttcccg ggagcaggtc
     2101 gaggagatag tagcgacccg cgcctacgac ctggagtaca aagaggtcta cccggacgtg
     2161 gatttcgctt tcgggcccgt tagccgcggg cctcgggagt agcgtcgtgg aaccagagaa
     2221 tatgctcgca ggcgcaacgg tcagggtacg cgagcgccac aggatcgcgg agcgccgcgg
     2281 catggtgggc agggtcacgg gtcgttacgg cggagaaggg tacgtggcct tggacgtgcg
     2341 cttctcgggc ggacggcaga ggttgttccg cccccaggac ctggaggaga tctcaccccc
     2401 ccaaccctgg tggcgctcgc tgttaaatct agagaggacc cgaaacagaa ccgactccag
     2461 gctccgcact ctcgattcct gacccacctg gcgggtctgg tcgcggccca ggtaggcgta
     2521 ggcaaagccg gtgccatagc ccctccggtg cgttctttgc ggcggaacgg atttgggcgg
     2581 ttcacgaacc gcccctacag gggtgtgggt ggatcatcgt gtgttgcgcg cggtatgtgg
     2641 ttggacggct cgcgaagcgc ccttcagggt ttcgtggtgg gggcgctcat gggaggattt
     2701 tgtcgggggt tatggggagg tcccggacgc gtatcccggt ggcgtggtag acggcgtttg
     2761 ctatggcggc ggcggagccc acgatgccga tctcacctat ccccttggag cccatggggt
     2821 tcacgtaagg gtcgtcctcg tctatccagg acacgtctat ctcgcccacg tcggcgttgg
     2881 tagggatgtg gtactcggcg aggtcctggt tcaccacgtg accgaagcgc ccgtccacca
     2941 ggctctcctc gtgcagcgcc atagagaggc ccatggccat gcccccgatg aactgtgacc
     3001 tggcggtccc tgggttgatg acgttgccga cggcgaaaac cccggtcatc cgtgggacgc
     3061 gcacctcccc cgtgtccgcg tccacccgcg tctcgacgaa ataggccccg aaggcgtgca
     3121 tggcgtagtt ctccgtctcg gggttgttgg gggtacgggc ctggacctcg tcgccctccg
     3181 aagggtcttc gccgtgccgc tcgcggaaca cgcgggccgc ctcgaagatg gccgcgcccc
     3241 acgccgtgat ccccgaggag ccgccctcca cggaggcgta gggcagcctg gtgtccccga
     3301 tccggaggtc cacgtcttcg acgccgatct cgagggcgtc ggcggagatc tgggttagcg
     3361 ccgtccaggt gccggtgccg atatctgcgg ccccgatctc cactgcgtac ctcccatctc
     3421 cttcgtagcg gatggtcgcc cgggatcccg gcatccggaa gacgggatag gtggccgagg
     3481 caaccccggt gccaacgagc catcgccctt cctgcctggt tcggggggcc gggtcgcggt
     3541 cctcccatcc aaagcgccgc gccccttccc tcaggcaggc cacgaggttg cggctggagt
     3601 acgggagccc ggactccggg tcgatctccg gctcgtttcg gacgcggaac tcgatggggt
     3661 cgagattgca ggcctcagcc agctcgtcca tggccgactc cagcgcgaac atgccggggg
     3721 cctcgcccgg tgcccgcatc cacgagggca cgggtacgtc gagcgcggcg aggcggtggg
     3781 tcgtgcggcg gctctgcgtg gcgtacatca tccgggagac aaccgcggtc tgctcggcga
     3841 actccttgaa cctggccgtc tgctctatca cgtcgtggcc taccgccgtg aggtgtcctt
     3901 cttcgtccgc cgcgaggcgc atcctctgca ccgtcggggt gcggtacccg acgcccgcga
     3961 acatctgctg gcgcgtgagg gcgaacttga tcggccgccc ctcgaccatc tgcgccgcga
     4021 gcacggcgag catggtgttg gcgtggggca ggcccttcga gccgaaccca ccacccacgt
     4081 acggcgcgac gacccgcacc cggtcccggt ccagcccgaa gaccctggcg accgttctgc
     4141 cgacggggtg tacaccctgg gttgactcgt acagggtcag gtttccaccc tcccagatcg
     4201 cgaccgtggt gtgcggctcc atcgggttgt tgtgctcggt cggcgtcgtg tacgtctcgt
     4261 cgagcctcac cgccgcggag gagaaagaac gatcgaaatc tccttcttcc gtgtccgtgg
     4321 gaaacccggc gttcaccacc tccggtgcgt acagaccgtc ggatgccggg tcgagcacgg
     4381 cggcgtgtgt ctcttcctcg taggagatct cgacgaggct tgcggcatgc ctggccacct
     4441 cgtaggtctc ggcgatcacg gccccaacgt actggccacg gtagtcgacc cggtcggatt
     4501 ggagcgtcgc gagttcctga tctcttgtgt cggccatccg cggcgcgttc tcgtgggtta
     4561 cgacgatgac cacgccagga acgtcttgag ccctgctgac gtccatgctg acgatgcggc
     4621 cgcgggcgat gctgcttcgc accgcgaaca ggtagagcgg gtgttccacg tgatgctcgt
     4681 aagcgtaggg ggccgtgccc tttacctttt gcggcccgtc gatgcggtcg aggggcatgc
     4741 cgatcgccct gcctgtcgcc cgttctgtcg tgctcatcgt ccctccgaaa gctcgagcag
     4801 cgtacgggtg atgacgttac gcgcgagcgg caccttgaag gcgttgtcct gcaacggttc
     4861 ggcgctctcc agctcgatgt ccgcggcggc gcggaagttc tcctcgcccg ccggttcgcc
     4921 tctcagggcc tcctccgcct tcgtggcgcg ccagggtttg tgggccaccc cgccaagggc
     4981 gatgcggacg tcgcgcacca cgccgtcttg cacgtcgagt gccgcggcga ccgagacgag
     5041 ggcgaaggcg taggaggccc gctcgcgcac cttgcgatac ttcgagttgg agaggaaccc
     5101 agggggcggc aggtccaccg aggtgatgag ctcgccgtgc tccagcaccg tgtcgtgccg
     5161 gggctcgtcg cccggcaggc ggtgcaggtc ctctatcggg atgcggcgcc cgccctcggg
     5221 gccacgcacg ttgacgaacg catcgagcgc ggccatcgcc acggccatgt
//
LOCUS       DBNGVD010000314         8309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2623854, whole genome shotgun sequence.
ACCESSION   DBNGVD010000314 DBNGVD010000000
VERSION     DBNGVD010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2623854"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1260)
                     /gene="obgE"
                     /locus_tag="AC16CM_08375"
     CDS             complement(19..1260)
                     /gene="obgE"
                     /locus_tag="AC16CM_08375"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3139887.1"
                     /translation="MQFVDEASFVVRGGRGGDGSVSFNREKYKPRGGPDGGRGGDGGS
                     VILHATEDLSTLEPHAYRNVVRAGRGVHGSGNNRAGERGRDVTLDVPVGTQVFDINGL
                     LADLSEPGETFVVAGGGEGGRGNGSFATSTRRAPAFRERGLPGEEREIRLELKVLSDV
                     GLVGLPNAGKSSLLRALSAARPRVGDYPFTTLAPQLGVVDEKTYRGPFVVADIPGLIS
                     GASAGKGLGNRFLRHVARARLLALVLDASEDPEGAEGTLRAELHAARLTAKPTVVVLN
                     KVDLLDGELRSYLREAFPGASQVSASTGEGVVELRDRLEETLRGMGDQGGHDDTIERR
                     HRVFRPTWKGVRVEPENDGYVVSGEEVERLALKTDWGNAEGVERFQYELERAGVVGAL
                     RRAGAESGDEVRIGETVFDFQ"
     gene            complement(1292..1567)
                     /gene="rpmA"
                     /locus_tag="AC16CM_08380"
     CDS             complement(1292..1567)
                     /gene="rpmA"
                     /locus_tag="AC16CM_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3139888.1"
                     /translation="MAHKKGGGSSRNGRDSAGRRLGVKAYGGQMVTAGSIIVRQRGSK
                     VHPGPNVGKGSDDTLFAKIDGRVDFGRRRGKSVVSVVEAPVLEEATA"
     gene            complement(1557..1856)
                     /gene="rplU"
                     /locus_tag="AC16CM_08385"
     CDS             complement(1557..1856)
                     /gene="rplU"
                     /locus_tag="AC16CM_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3139889.1"
                     /translation="MFAVVKSGGKQYRVTEGQELVVDRISGDVGDSVELPVGFTADES
                     GFDLADRTARVEILEHLRGNKIHIYKYKPKKDHRKKTGHRQELTRVRVLEVSDGS"
     gene            2120..3286
                     /gene="rodA"
                     /locus_tag="AC16CM_08390"
     CDS             2120..3286
                     /gene="rodA"
                     /locus_tag="AC16CM_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3139890.1"
                     /translation="MQSIRITSAGPVVRNKKQDKASIGQRLRRADFLLFLSALALGGI
                     GVLAIYAAEADYRQLYAINQGLGFSVGFVGAVVLALLDYRWLERHLRLVYGTIIVMLV
                     AVVLFGVTVNGSQSWLSIGPVHLQPSEFAKPLAIVVLAGFVAHNSFTYNMTFIKAIAL
                     LAVPILLVLAQPDLGTALVFAAIFVTMIYVGGARWYQLAGLFLAGCVAVYLAIRFRIL
                     EEYQIARLTSFLDPQSDPQVSYQVENSKMAIGSGGFTGKGINSSGTLGDLGYLPEDRT
                     DFIFANLAEKVGFVGAMLVLVIFFVLVWRVLHAASVSRDRFGVLIAVGVATMLTFHAL
                     INIGMAMGMMPVTGLPLPFVSYGRSNLLVSMMSVGLVQSIVIQARLKSEVSPRT"
     gene            complement(3545..4063)
                     /gene="mreD"
                     /locus_tag="AC16CM_08395"
     CDS             complement(3545..4063)
                     /gene="mreD"
                     /locus_tag="AC16CM_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3139891.1"
                     /translation="MEQSTVVRAAVVVAVAALLDAVVVPYLTFGYFSPHLTLVAVVFV
                     AAPLRDLQAVLLGFFGGVLLDALGGDLFGVGALGGLIAAALASRVSAVRRRGAERILL
                     AQVAGVSVAIYDLLGYAARWLSGLSVPQLGEYTLWGILPDVLINALIALLIGGWLLRV
                     VDPKPQHSWDGG"
     gene            complement(4073..5002)
                     /gene="mreC"
                     /locus_tag="AC16CM_08400"
     CDS             complement(4073..5002)
                     /gene="mreC"
                     /locus_tag="AC16CM_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3139892.1"
                     /translation="MGQKKPGAVSGLATLLILCVASLALFTVFVKEGDCEQGGECGPL
                     HTVQLGAAEVLQPVRSVFAVILSPVTNTGERLMSAFDDSREQELERELRRTEELAAQT
                     SRLERENAELRELLEGQRSAYQYGPLAQVVAPVGDQFTQRIIINVGTQDGVESNMPVV
                     IGQSTLVGRTTEVSRHTAQVMLITDQMFAAGVRIIPPARFDPSSGEISPAVTDESTSY
                     GQGLLGTDLEGYLGVDLVSTSAHAEEGDFIVTSGRAGDKELLFPPGLYVGNVESVSSQ
                     DIDQFKKIVVEPAVGPQDLEQVRVITDWAGRNG"
     gene            complement(5014..6042)
                     /locus_tag="AC16CM_08405"
     CDS             complement(5014..6042)
                     /locus_tag="AC16CM_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3139893.1"
                     /translation="MFGGLFGRDIAIDLGTANTLVFVKGRGIVLSEPSVVAIDTKTDK
                     VVAVGSTAKRMLGRTPSNIVAMRPLKDGVIADFEITEKMLAYFIRKVQPQRMLFRSLV
                     GPRVVVCVPSGVTGVELRAVKEATESAGARQAYTIEEPLAAAIGAGLPVNEAQGSMVV
                     DVGGGTSEVAVLSLGGIVTKSSIRIAGDDIDEAISTYIQKEYKLAIGTQTAEQLKIEL
                     GSAFRLEEEESAEIRGRDLVTGLPKTVVITSEEVREAISVPVDAILAAVRDTLDRTPP
                     ELASDIMDRGMILAGGGALLRHLDERLRRETGIPVHVADDALMCVAIGSGRSLEEIDF
                     YRSALASA"
     gene            complement(6092..6778)
                     /gene="radC"
                     /locus_tag="AC16CM_08410"
     CDS             complement(6092..6778)
                     /gene="radC"
                     /locus_tag="AC16CM_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3139894.1"
                     /translation="MDRRRYTIKELPPELRPRERLLAEGPEALSAAELLGILLGIGSR
                     EQTAVELGQQVISESGGLFGLHGSMVHDLRKVHGIGEAKACIVLAAVEFGKRLGRVRN
                     PGRPVISSPEDVDGLLRGRIAHLDRENFVVVLLNTKNEVLSYPTVSVGTLSASLVHPR
                     EVFKPAIRASAAGVILAHNHPSGRVGPSREDREVTRRLKEASEIIGIEVLDHVILGDG
                     YFSMKEHGIL"
     gene            complement(6810..7382)
                     /locus_tag="AC16CM_08415"
     CDS             complement(6810..7382)
                     /locus_tag="AC16CM_08415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3139895.1"
                     /translation="MHFVLASESARRVDLLRTAGYEFQPQKSGFPEVTLDDPVETVNA
                     NASGKALAIERDPETVVLAADTIVYLPNLPAGERILGQARDGDDVTRFLGFLQGRAHE
                     VYSGVAVLKGDDLAVSHAVTTVKVQNLDAPRIASYARYGEGVGKAGGYAIQGRAGSFI
                     EWIGGDYTNVVGLPLTLTGRMLANAGIPHP"
     gene            complement(7392..7793)
                     /gene="ndk"
                     /locus_tag="AC16CM_08420"
     CDS             complement(7392..7793)
                     /gene="ndk"
                     /locus_tag="AC16CM_08420"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3139896.1"
                     /translation="MEDTLVLIKGDGVRRKLIGEIIRRMEAKDLDIADMKLMDVNREL
                     AEEHYAEHREKPFFDELVDFITSTPVVAMRVRGEGAIKVMRSLMGSTNPAEAAPGTIR
                     GDLALSMPDNLVHGSDSPESAGRELGLFFGT"
     gene            complement(7804..>8309)
                     /locus_tag="AC16CM_08425"
     CDS             complement(7804..>8309)
                     /locus_tag="AC16CM_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3139897.1"
                     /translation="YEAFPMISSIVLLQDPFQMVSDFLASPILRISGQLVALLFVVLW
                     LALVYWTFADATRRGAVRILWGLVAVVFPYVGTLIYLIVRPHEYLDESRERELETAVL
                     ERELRNSVLLCPNCRNMVEKDFLVCPSCRWELKKPCEKCDRPLNMEWETCPYCGTDQR
                     SDQTITR"
BASE COUNT         1402 a         2777 c         2564 g         1566 t
ORIGIN      
        1 cctatcttca agtccacctc actggaagtc gaacaccgtc tcgcctatcc tgacctcgtc
       61 gcccgactcg gcgccggccc gccgaagggc gccgacaacg ccggcccgct ccagctcgta
      121 ctggaacctc tcgacgccct ccgcgttgcc ccagtcggtc ttgagcgcga gccgctcgac
      181 ttcctcaccg gagacgacgt agccgtcgtt ctccggctcg accctgacgc ccttccaggt
      241 cggcctgaag acgcgatgcc gccgttcgat ggtgtcgtcg tgcccgccct ggtcccccat
      301 cccgcgcagc gtctcctcta acctgtcgcg caactcgacg acaccttcgc ccgtggaggc
      361 ggagacctgt gaggcccccg ggaatgcttc ccgaaggtac gaccggagct ctccatcgag
      421 caggtccacc ttgttaagaa ccacgaccgt gggcttggcg gtgagccttg ccgcgtggag
      481 ctcggcccgc agcgtcccct ccgccccctc cgggtcctcg ctcgcgtcga gaacgagggc
      541 gagcaacctc gcgcgcgcca cgtgccgcag gaagcgattg ccaagcccct tgcccgcgct
      601 cgcgccggag atgagccctg gaatgtcggc gaccacgaag ggtccgcggt aggtcttctc
      661 gtccacgacc ccgagctggg gcgcgagggt ggtgaaggga taatcgccga ccctgggacg
      721 cgcggcgctg agggcgcgaa gcagggatga cttgcccgcg tttggcagcc cgacgagccc
      781 cacgtcggag aggaccttga gctccagacg gatctcacgt tcctcgcctg gcaggcccct
      841 ctccctgaac gccggcgccc tacgggtgga ggtggcaaag gaaccgttgc ccctgccgcc
      901 ctcgccgccg ccggcaacca cgaaggtctc ccccggctcg gaaaggtccg cgagcaggcc
      961 gttaatgtcg aatacctgcg ttccaacggg cacatccagc gtgacgtccc gaccgcgctc
     1021 gccggccctg ttgttaccgg agccgtggac cccgcgcccg gccctgacca cgttgcggta
     1081 ggcgtggggc tcgagggtgg agagatcctc cgtggcgtgc aggatcaccg atccgccgtc
     1141 gccgccgcga ccgccatcag ggccgccgcg cggtttgtac ttctcgcggt tgaatgagac
     1201 gctgccgtcg cccccacgcc caccacggac gacgaagctg gcttcatcta cgaactgcaa
     1261 aggggctaga gacccccgta acggaaggtg gtcaggccgt ggcctcctcc agaacgggcg
     1321 cctcgaccac gctgacgacg ctcttgccgc ggcggcggcc aaagtcgacc ctgccgtcta
     1381 tcttggcgaa gagcgtatcg tcggagccct ttccgacgtt cggccccggg tgaaccttcg
     1441 agccgcgctg gcgaacgatg atgctgcctg cggtgaccat ctggcccccg tacgccttta
     1501 ccccgagcct ccggcccgcc gagtcgcggc cgttgcgcga tgagcctcca ccttttttat
     1561 gagccatcag acacctccag aaccctcacc cgggtcagct cctgcctgtg cccggtcttc
     1621 ttcctgtgat ccttcttcgg cttgtacttg tagatgtgga tcttgttccc ccgcagatgc
     1681 tccaggatct ccaccctggc cgtccggtcg gcgaggtcga acccgctctc gtccgccgtg
     1741 aagccgacgg gcagctcgac cgagtcgccc acgtcgcccg agattcggtc gacgaccagc
     1801 tcctggccct ccgtcaccct gtactgcttt cctccgcttt tcacaaccgc aaacattacg
     1861 ttctcctaag atttcgagct acgataggcc tacgaaacga acccataatg ccacagtata
     1921 cccgccatgt agcgtatcga ccagcacgtg tggcgctctg atccaggctc tccttctcgc
     1981 cgcggattcg tgcaaaagtg gggattgacg aggtctggca gaaccggcga gcaggagagc
     2041 gcgttcagga cctgcggacc cgacgctcga ttttctaacc ggcgttgccc ctgggtggac
     2101 tattcggtat ctttgggcca tgcagtcgat caggataact tccgcgggac ccgtcgtccg
     2161 gaacaagaag caggataagg caagcatcgg gcagcgtctg cggcgtgccg actttctact
     2221 gtttctgtcc gcgctggctc tgggcggtat cggggtactg gccatctacg cggccgaggc
     2281 ggattacagg cagctctacg ccatcaacca ggggttgggg ttctcggtcg ggttcgtggg
     2341 tgcggtcgtg ttggccctgc tcgattaccg ctggctggaa cggcacctca ggctggttta
     2401 cggtacgatc atcgtgatgc tcgtcgccgt ggtgctcttt ggcgtaacgg taaacgggtc
     2461 gcagagctgg ctgagcatcg gccccgtaca tctgcagccc tcggagttcg cgaagccgct
     2521 cgccatcgtg gtgctcgccg gctttgtcgc gcacaacagc ttcacctaca acatgacctt
     2581 catcaaggct atagccctcc tggcggtgcc catcttgctg gtgctggccc agccagacct
     2641 ggggaccgct ctggtgttcg cggccatctt cgtgaccatg atctacgtgg gcggtgccag
     2701 atggtaccag ctcgcaggcc tgttcctcgc gggttgcgtg gccgtatacc tcgcgatccg
     2761 attccgaatc ctggaggaat accagatagc ccgcctcacc tcgtttctgg accctcagag
     2821 cgaccctcag gtcagctacc aggtcgagaa ctccaagatg gccataggct cggggggatt
     2881 cacgggcaag gggataaact cgtcgggaac cctcggcgac ctcggctacc tgcccgagga
     2941 ccgaaccgac ttcatcttcg ccaacctggc agagaaggtc ggcttcgtcg gggccatgct
     3001 ggtgctcgtg atcttcttcg tccttgtctg gcgcgtactg cacgcggcct ctgtttcacg
     3061 cgaccgcttc ggggtgctca tagccgtggg cgtcgctact atgctgacct tccacgccct
     3121 gataaacatc ggcatggcca tgggcatgat gcccgtaacc ggcctccccc taccgttcgt
     3181 gagctacggc cggagcaacc tgctggtgag catgatgtcg gtcggcctgg ttcagagcat
     3241 agtgatacag gccaggctca agtccgaggt ttcccctcgg acttgagcgg cacgctgcct
     3301 ggcggcagct tgtcagcaca ctcgggctag cgccctcgct tgtcggcaac gcttcggctt
     3361 cgcctcgctt tcggctagtc gtctctctcc gtaacggcag cttcggcaac ctaccttccc
     3421 tgcgtaacaa tgaattcggg cggataggtg caggccgcag cgggatctgg ctgacagcga
     3481 gcctggcgaa gccgggcgaa gcgttgctga caagcgtagg cccgaagggc cgtagcgcgc
     3541 ggcgttagcc gccgtcccag ctgtgttgcg gctttgggtc gactactctc agcagccagc
     3601 cgccaatgag gagtgctatc agggcgttga tcaggacgtc ggggaggatg ccccagaggg
     3661 tgtattcgcc cagttggggc acggagagac ctgacagcca tcgggcggcg taacccagaa
     3721 gatcgtagat tgccaccgag acgccggcta cctgggcgag gaggatcctt tcagcccctc
     3781 tgcgacggac ggcgctgaca cgggaggcaa gggctgcggc tatgaggccg ccgagggcac
     3841 ccacgccgaa gagatcccca ccgagggcgt cgaggagcac gccgccgaag aagccgagca
     3901 ggacggcctg caggtcccgc agtggggcgg cgacgaagac caccgctacc agggtgaggt
     3961 ggggcgagaa gtatccgaag gtcaggtacg gaacgactac agcgtcgagg agggcggcga
     4021 cggccacgac gacggcggcg cgtacgacgg tggactgctc caaatggggt ccctagccgt
     4081 tcctgccggc ccagtcggtg atgaccctga cctgctccag gtcctggggc cctacggcgg
     4141 gctcgaccac tatcttcttg aactggtcga tgtcctgaga gctcaccgac tccacattgc
     4201 ccacgtagag gccgggtggg aagaggagtt ccttatcccc ggcccgcccg ctcgttacta
     4261 tgaaatcgcc ctcttcggcg tgggcgcttg tgctgaccag gtcgaccccc aggtatccct
     4321 ccagatcggt gcccagaagg ccctggccgt aggaggtgct ctcgtcggtc acggcggggc
     4381 tgatctcccc agatgacgga tcgaacctgg caggcggaat aatcctgacc ccggccgcga
     4441 acatctggtc ggtaatcagc atgacctggg ccgtgtgcct cgagacctcg gtggtgcgcc
     4501 cgacgagggt gctctggccg ataaccaccg gcatgtttga ctccacgccg tcctgggtgc
     4561 ccacgttgat tatgatcctc tgggtgaact gatcgcccac gggtgcgacg acctgcgcga
     4621 ggggaccata ctggtaggcc gaccgctgtc cctccaggag ttcgcgcagc tcggcgttct
     4681 cgcgctccag gcgcgaggtc tgggccgcca gctcttcggt ccggcgcaac tcacgctcca
     4741 gctcctgttc cctgctgtcg tcgaaggcgc tcatgagcct ctcgccagtg ttcgtcaccg
     4801 gcgagaggat cacggcgaag acgctcctga caggctgtaa tacctcggcg gccccaagct
     4861 ggaccgtgtg caggggccca cattcgccac cctgctcgca gtcaccctct ttcacgaaga
     4921 ccgtgaacag cgcgaggctt gccacgcaga ggatcagcaa cgtggcgagg ccgctaacgg
     4981 cgcctggttt cttctgtccc accctctcgc ccgctaggcc gacgcgaggg cgctgcggta
     5041 gaagtcgatc tcttccagcg acctcccgct gcctatagcg acgcacatca gggcatcatc
     5101 agcaacatgg acagggatcc ccgtctcgcg cctcagccgc tcgtcgaggt gccgcagcag
     5161 agccccgccg ccggcgagga tcatgccacg gtccatgata tccgaggcga gctcgggggg
     5221 cgtgcggtct agcgtgtcgc gcacggccgc gaggatcgcg tcgaccggca cgcttatggc
     5281 ctccctgacc tcctcgctcg tgatcacaac ggtcttgggc aatcctgtga cgaggtcccg
     5341 gccccggatc tcggccgact cctcctcctc cagccggaaa gcgctgccca gctctatctt
     5401 gagctgctcg gcggtctgcg tgccgatggc gagtttgtac tccttctgga tgtaggtgga
     5461 gattgcctcg tctatgtcgt ccccggcgat gcggatggag gactttgtga ctatgccgcc
     5521 gagggagagg acggcgacct cgctggtccc gccaccaacg tcaacgacca tcgacccttg
     5581 cgcctcgttg accggcaggc ccgcccctat ggccgcggcc agcggctcct cgatggtgta
     5641 ggcctgcctg gctcccgccg actccgtcgc ctcctttaca gccctcagct caacgcccgt
     5701 gacaccagat ggcacgcaca cgacgacgcg cggtccgacg agcgaccgga agagcatgcg
     5761 ctggggctgc accttgcgaa taaagtacgc gagcatcttc tccgtgatct cgaagtccgc
     5821 gatgacgccg tccttgaggg gccgcatggc gactatgttg ctcggcgtgc gccccagcat
     5881 gcgcttcgct gtggacccta cggccacgac cttatccgtc ttggtgtcta tcgcgaccac
     5941 cgagggctcg gacaacacga tcccccgccc cttcacgaac accagagtgt tcgccgtgcc
     6001 aaggtctata gctatgtccc tcccgaataa tcctccgaac ataccctacc catcctctgt
     6061 ttcgaactcc cccagcggcc ctaccccgac cctagagtat tccgtgctcc ttcatactaa
     6121 agtacccgtc gcccaggatc acgtggtcca gaacctcgat acctataatc tcggaggcct
     6181 ccttgagcct cctcgtgacc tcacggtcct cacggctagg cccgaccctg ccgctggggt
     6241 ggttgtgggc gaggatcacg cccgccgcgc tggcccggat tgccggtttg aatacctccc
     6301 tggggtgaac cagagacgcc gagagcgttc cgaccgagac cgtgggataa ctcagtacct
     6361 cgttcttggt gttcaagagc acgacgacga aattctcccg gtcgagatgg gctatccgcc
     6421 ccctcaaaag accgtcaacg tcctcgggcg atgatatcac gggccgtccg gggttgcgaa
     6481 ccctcccgag gcgcttgccg aactccacag cggccagaac gatgcacgct ttggcctccc
     6541 cgatcccgtg tactttccgc aagtcgtgca ccatagaccc gtgtaatccg aagagcccgc
     6601 cactctccga gatcacctgc tgcccgagct cgacagcggt ctgctcccgg ctgccgatcc
     6661 caagcaagat cccgagcaac tccgccgccg agagcgcctc gggaccttca gccagaagcc
     6721 gttccctggg cctgagctcg ggcggaagct ccttgatggt gtaccgccga cgatccatgt
     6781 tcgtatatta tattatacag aggatggggt caggggtggg ggatgccggc gttggctagc
     6841 atgcgtccgg tcagggtcag gggtaggccg actacgttgg tgtagtcgcc gccgatccac
     6901 tctatgaagg agcctgccct gccctggata gcgtagcccc cggccttgcc tacgccctcc
     6961 ccgtatcggg cgtaggaggc tatgcgtggt gcgtcgaggt tctggacctt taccgtggtt
     7021 acagcgtggc tgacggccag gtcgtcgccc ttcaggacgg cgaccccgga atagacctcg
     7081 tgggcgcggc cttgcaggaa gccaaggaac cttgtgacat catctccgtc tctggcctgg
     7141 ccgaggatac gttcgcctgc cgggaggttc ggcaggtaga cgatggtatc ggccgcgagg
     7201 acgaccgttt cggggtcgcg ttcgatggcc agcgccttgc cgctcgcgtt ggcatttacg
     7261 gtctctacgg ggtcatccag ggttacttcg gggaaaccgc tcttctgggg ctggaattcg
     7321 tagccggcgg tgcggagcag gtccacccgc cgggccgact cggaggccag gacgaagtgc
     7381 acgacctggg atcaggtgcc gaagaagagg cccaactctc gcccggcgct ctctggggag
     7441 tcggagccgt ggaccaggtt gtcgggcatg ctgagggcca ggtcgcccct gatggtgcca
     7501 ggggccgcct cggcggggtt ggtggagccc atcagggatc tcatcacctt gatggcacct
     7561 tcgcccctga cccgcatggc gaccacgggg gtcgaggtga tgaagtccac aagctcgtcg
     7621 aagaacggct tctcgcggtg ttcggcgtag tgctcctcgg ccagctcgcg gtttacgtcc
     7681 atcagcttca tgtcggcgat gtcaaggtcc ttcgcctcca tgcggcggat gatctcgccg
     7741 atcagcttgc ggcgcacgcc gtcgccttta atcagtacca gggtatcttc cattatcttc
     7801 tccttatctt gttatcgtct ggtcgctgcg ctggtcagtg ccgcagtagg ggcacgtctc
     7861 ccattccatg ttcaggggcc tgtcgcactt ctcacagggt ttctttagtt cccatctaca
     7921 cgaggggcag accagaaaat ccttctcgac catgttgcgg cagttagggc aaagcagcac
     7981 gctgttcctg agctcgcgct cgagcacggc cgtctcgagc tcgcgctcgc gggactcgtc
     8041 caggtactcg tggggacgca ctatgaggta gatgagggtt ccaacgtagg ggaagacgac
     8101 agccaccagg ccccacaaaa tgcgtacggc cccgcggcgg gtggcgtcgg cgaacgtcca
     8161 gtagactagg gccaaccaca gcaccacgaa caggagcgcc accagttgac cggagattct
     8221 gaggatcggg ctcgccagaa agtcggagac catctgaaac gggtcctgca gcagcacgat
     8281 agacgagatc ataggaaaag cctcatagg
//
LOCUS       DBNGVD010000315         4154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4080365, whole genome shotgun sequence.
ACCESSION   DBNGVD010000315 DBNGVD010000000
VERSION     DBNGVD010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4080365"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16CM_08430"
     CDS             <1..333
                     /locus_tag="AC16CM_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3139898.1"
                     /translation="TLMTAGLGKQAITHFEVLDHADRHTMLRVRLETGRTHQIRVHLS
                     AIGYPVYADPLYGTPVPDRRLWLHAEQLSFQHPVTGQALEFHSEIPADLRDSAISLEV
                     DLPDEARS"
     gene            complement(423..1214)
                     /locus_tag="AC16CM_08435"
     CDS             complement(423..1214)
                     /locus_tag="AC16CM_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139899.1"
                     /translation="MNALSLWVGDSATMLRRQLRHMQRYPSLVVLLVSVPVILLLLFV
                     YVFGETMGAGLGGPSGGRGAYADYVTPGIILITVASVAQGTAISVAMDTTGGIVTRFR
                     TMAISRASVLTGHVLGAMTKTMLAVAVVVGVAMLVGFQPTATALEWLAAVGVLAMVSW
                     ALTWLTVALGLVTDSAETASNLPMPLILLPFFGSGFVPTESMPDWLRWFAEYQPFTPI
                     METLRGLLLGTAIGNSAILAVAWCASISLVGFLWAKALFNRDSTP"
     gene            complement(1211..2134)
                     /locus_tag="AC16CM_08440"
     CDS             complement(1211..2134)
                     /locus_tag="AC16CM_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3139900.1"
                     /translation="MGLRKSFGGQVVLDGIDLEVAEGTVFALLGPNGAGKTTTVNILS
                     TLITADGGDIRVAGHDPAREPDAVRRSIGVTGQFSAVDDLLTGEENLRLMADLGHLGR
                     AEGRRRAAGLLERFDLVQAAKKTPPTYSGGMRRRLDLAMTLMVDPRVVFLDEPTAGLD
                     PRGRRTMWRIIRDLVADDVTIFLTTQYLEEADQLADQIAILDRGTLVAQGTGYELKRR
                     IPGGHVRLEFADARGLESAARIVGGSTREEDALALRVPTDGGVGSLKTMLDRLEGGGI
                     PVEEISMHTPDLDDVFLALTGHPETGKDAPR"
     gene            2551..3648
                     /locus_tag="AC16CM_08445"
     CDS             2551..3648
                     /locus_tag="AC16CM_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR3139901.1"
                     /translation="MTKESKEREELLRELVEENRRSNVEGLSYLQAVAERSGMNLSDL
                     QCMNILALTGPVTAGRLGEEMGLTTGAVTGLVNRLEKSGYVRREKDPDDGRRVVIRPV
                     AEELERAGLVYPGSREEMSLNSLLSDYDDRTLAIFLEVVRKSNRATREETARIRAASR
                     GGDREGGFSAPLGSVKSGRLVFAKGADRLTLRASSGMGDLYRGRFEGVLPKVDVKDGT
                     VTFRYSSGFGGLFERRSRPGEVTLNTAIPWEVEVRGGAYKVEADLRGSELSSFILKGG
                     ISHLDLRLPEPSGVVPIRLSGGASKVTIQRPLGVGARLNLKSGAAALTFDEQSFDAVG
                     GTVRLQSEGYDGASERYEIEVSGGASDLTVR"
     gene            3816..>4154
                     /locus_tag="AC16CM_08450"
     CDS             3816..>4154
                     /locus_tag="AC16CM_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139902.1"
                     /translation="MSGAAANGEGAATRWVVILCWVAVAMDGFDLVVLGAVAPALLEY
                     REWGLTAPQVGAITSYGLFGMLIGALGIGTLSDVIGRKRSILVSVAGFSVFTALCAVA
                     PTPGIFGMLRF"
BASE COUNT          732 a         1273 c         1417 g          732 t
ORIGIN      
        1 accctcatga ccgcgggcct gggcaaacag gccatcacgc acttcgaggt cctggaccat
       61 gcggaccggc ataccatgct ccgagtccgg ctcgagacgg gaaggacaca tcagatccgg
      121 gtccacctct cggctatcgg gtacccggtg tacgccgacc ctctgtacgg aacgcctgtc
      181 ccagaccgcc gtctctggtt acacgcagag cagctatcct tccagcatcc tgtaaccggg
      241 caagctttgg agtttcactc ggagatacca gcggatctgc gcgactcggc catctcgctc
      301 gaagttgacc tccctgatga agccaggtca tgaggtcccg gctagctctc cgtccgagac
      361 cctggaggac cgtgttcttc gcatcggccc ggggtatgca ggccccgggc cgcacgtctc
      421 ggctaggggg tggaatcccg gttgaacagc gccttcgccc agaggaatcc gaccagcgag
      481 atggacgcgc accaggcgac ggcaaggatg gcgctattgc ctatcgcggt acccaggagt
      541 agcccccgca gtgtctccat gatcggggtg aaaggctgat attcggcgaa ccagcgcagc
      601 cagtcgggca tcgactcggt tgggacgaac ccgctgccga agaacggcaa aagtatcagg
      661 ggcatgggca ggttgctcgc ggtctcggcg ctatcggtga cgaggccgag ggcgaccgtg
      721 agccaggtga gcgcccagga caccatcgcc agcacgccga ccgccgcgag ccattcgagg
      781 gcggtggccg tgggctgaaa accgacgagc atcgccacgc cgacgacaac ggcgacggcg
      841 agcattgtct tggtcatggc gccgaggacg tggccggtca ggaccgaggc gcgggagatc
      901 gccatggtgc ggaaccgggt gacgatgccg cctgtcgtgt ccatggccac ggagatggct
      961 gtcccctggg ccacggaggc cacggttatg aggatgatgc cgggggtgac gtaatcggcg
     1021 taagcgcctc gtccgcccga cgggccgccg agcccggcgc ccatggtttc gccgaacacg
     1081 tagacgaaca ggagcaggag tatgacgggc acgctgacga ggagcacgac cagcgaaggg
     1141 tagcgttgca tgtgcctgag ctggcggcgc agcatggttg ccgagtcgcc cacccagagc
     1201 gagagcgcgt tcatcgtggg gcatcctttc cggtttcggg gtggccggtg agggccagga
     1261 atacgtcatc gaggtcggga gtgtgcatgg agatctcctc gaccggaatc ccaccgcctt
     1321 cgaggcgatc gagcatggtc tttagcgatc cgacgccgcc gtcggtcgga acccgcagcg
     1381 cgagcgcgtc ttcttcgcgc gtgctccccc cgactatcct ggccgccgat tcgagcccgc
     1441 gagcgtccgc gaactcgaga cggacgtgcc cgcccgggat gcggcgcttg agctcgtacc
     1501 cggtcccctg ggcgaccagc gtgccgcggt cgaggatcgc tatctggtcg gccagctggt
     1561 cggcctcctc caggtactgg gtggtcagga agatggttac gtcgtcggcc acgaggtcgc
     1621 ggataatccg ccacattgtg cggcggccgc gcgggtcgag gcccgcggtc ggctcgtcga
     1681 ggaagacgac gcgcgggtcg accataagcg tcattgcaag gtcgagcctg cgccgcatgc
     1741 caccggagta cgtgggcggc gtcttcttgg ccgcctggac gaggtcgaag cgctcgagca
     1801 atccggccgc gcggcgccgg ccctcggcac ggcccaggtg acccaggtcg gccatcagcc
     1861 tcaggttttc ctcgccggtg agcaggtcgt ccaccgccga gaactgcccc gtgacgccta
     1921 tagaccggcg gaccgcgtcg ggctcccgcg ccggatcgtg gcctgcgacg cggatgtcgc
     1981 cgccgtcagc ggtgatgagc gtggagagga tgttcaccgt cgtggtcttg ccggcaccgt
     2041 tggggccgag cagcgcgaat acggtgccct ccgcgacctc caggtcgatg ccgtctagca
     2101 cgacctgccc cccgaacgac ttgcgtaagc ccatagccga gatcgccgca ggacccgatt
     2161 caaaccccgc ctcccgtaac ccggggccgc cggacagcgc cccacccacg gggcccgatc
     2221 tatagtcgtt catctgtaac ctccttctaa ggatgtgcgg gctaacgacc cggactacgc
     2281 tgccccatgc gggctgcttt cctcgcagct gcagacccgg aggtaggctc cccaacgaga
     2341 cccttctcta gcgcccgcgc ccccggccca gcggtgcgcc acaattgcca ggggcccgtc
     2401 caactttctt cctcgatccc gatcctcctt cctcggaaaa ttatcgcgtg ctatacttca
     2461 tgtacttagt cactaatgta aaatcacaaa aatatattag taaataagat aaatacagga
     2521 taagctgtac cgaaagggtt gtcaagcggg atgacgaaag aatcgaaaga gcgggaggag
     2581 ttgcttcggg agctcgtgga ggagaaccgt aggagcaacg tggaggggtt gtcctatctg
     2641 caggccgtgg cggagcgcag tgggatgaac cttagcgact tgcagtgcat gaacatcctg
     2701 gcgttgacgg ggccggttac ggccgggcga ttaggggagg agatggggct cacgacgggg
     2761 gcggtgaccg gtttggtcaa ccgactagag aaatccggtt acgttcgccg ggagaaggac
     2821 ccggacgacg gtaggagggt ggtcatccgg cctgtagcgg aggaattgga gcgggcgggg
     2881 ttggtttacc ctggttcgcg agaggagatg tcgctcaact ctttgctctc tgactacgac
     2941 gatcggacgc tcgcgatatt tctggaggtc gtgcggaagt ccaaccgtgc gacccgggag
     3001 gagactgcca ggatccgggc tgcatccagg ggcggcgacc gagagggcgg gttctccgcc
     3061 cccctgggct cggtgaagag cggccgcctg gtgttcgcca agggggccga ccggctcacc
     3121 cttcgcgcct cctcggggat gggagacctc taccggggac gcttcgaggg ggttctcccg
     3181 aaggtcgatg tgaaagacgg gaccgtgacc ttccgctatt cctcgggctt cgggggactc
     3241 ttcgagcgtc gcagccgtcc cggggaggtt acgctaaaca cagcgatacc gtgggaggtc
     3301 gaagtgcgcg gcggagcgta caaggtcgag gcggatctca gaggctcgga gctatcgtcc
     3361 ttcatcctaa agggtgggat cagccacctc gacctgaggc ttccggagcc atcgggagtg
     3421 gtcccgatac gtctctccgg tggcgcaagc aaggtgacca tacaacgtcc ccttggggta
     3481 ggggcacggc tgaacttgaa aagcggcgcc gccgccctga ccttcgacga gcagagcttc
     3541 gatgccgtcg gcggaacggt tcgtcttcag agcgagggtt acgacggagc ttccgaacgc
     3601 tacgagatcg aggtctccgg gggcgctagc gatctcaccg ttcggtgagc caacccacca
     3661 ctggcacgtg tgcctggtcg ggttgtcgac gccgagcact ccggctcccg aacagccacc
     3721 ctcgctgccg agtttcctag atggcgtctt cccgtactgg acgtatagca cgattatggt
     3781 cagaatatcc ggggaaggtg aagaaaggag ctgagatgtc cggggccgcg gccaatgggg
     3841 aaggtgcggc gacccgttgg gttgtgatct tgtgctgggt cgctgtggcg atggacggct
     3901 tcgatctcgt ggtgctcggg gcggtggccc cagcgcttct ggagtatcgg gagtgggggc
     3961 ttacggctcc gcaggtaggt gccatcacct catatgggtt gttcggcatg ctgatcgggg
     4021 cgctcggcat agggacgctc tcggacgtga tcgggcgcaa gcggtccatc ctggttagcg
     4081 tggccggctt ctcggtcttt acggcccttt gcgccgttgc cccgacgcct ggcatcttcg
     4141 ggatgctccg cttc
//
LOCUS       DBNGVD010000316         1716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2440850, whole genome shotgun sequence.
ACCESSION   DBNGVD010000316 DBNGVD010000000
VERSION     DBNGVD010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2440850"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16CM_08455"
     CDS             complement(<1..407)
                     /locus_tag="AC16CM_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR3139903.1"
                     /translation="MTLQGYSIPRTSTGKASLVPPPPWHYVGDFLVVDYWADPDAVRA
                     VLPEGLDPHPDPGRCAAVCADWQSCSEGGDELVEPIRSHYREFFIVVNALLDGEEVTT
                     CPFIWVDQDFALARGWIQGFPKKLGSIWMTRTFG"
     gene            complement(404..1327)
                     /locus_tag="AC16CM_08460"
     CDS             complement(404..1327)
                     /locus_tag="AC16CM_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3139904.1"
                     /translation="MISLRRIAHVSLRVADLDDATSRWSNQFGLVERERDDRKSRLAC
                     DDEPFCLELVAAEAPGIGHVAYELNRSCTPDDAGRHFENLGVDYSEREGGLEVADPEG
                     NRVIVSPYREPGSRWTAHARPAGDQPVGHPRKLGHVNFLTGDISSGVEFYTRVLGMRV
                     TDWLGDGGVWFHINGDHHVMALVDKGYSHLHHLAFDVVDIGQMRVALDHLGRHGRWLG
                     WGPARHGIGGNIASYVRIVEEECFVELYCDMEQLEAEHEPRRWPDNRYSSNTWGPLPP
                     RSYFRFDAAAVASERESLEMLGTPLPEEVRS"
     gene            complement(1324..>1716)
                     /locus_tag="AC16CM_08465"
     CDS             complement(1324..>1716)
                     /locus_tag="AC16CM_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3139905.1"
                     /translation="LFKPAADEMLRWATAVHYFRRTTTRDTVLADTEIPAGAKVTTWL
                     VSGNRDETVFDNPDAFDVGREPNKHMAFGPGGIHHCLGAHLARLEVRIAFEEMLKRVD
                     GIELTGPPERLRSNFFNGIKRMPVRVVR"
BASE COUNT          279 a          570 c          579 g          288 t
ORIGIN      
        1 ccgaaggtgc gggtcatcca gatagagccg agcttcttgg ggaaaccctg gatccaaccc
       61 cgcgccagcg cgaagtcctg gtccacccag atgaacgggc aggtggttac ctcctccccg
      121 tcgagcaaag cgttcaccac gatgaagaac tcccggtagt gggaccggat gggctcaacg
      181 agctcgtccc cgccctcgga gcacgactgc cagtccgcgc acacggccgc gcagcgtccc
      241 gggtcgggat ggggatctaa cccctctggc agcacggccc tcacggcgtc cgggtcggcc
      301 cagtagtcca cgacgaggaa gtccccgacg tagtgccacg gcggcggggg gacgaggctc
      361 gccttgcccg tgctcgtacg tggtatggaa tatccttgca acgtcatgat ctgacctcct
      421 cggggagcgg tgttccgagc atctccaggc tctcgcgctc ggaggcgacg gccgcggcgt
      481 cgaaacggaa ataggaacgg ggtggcaggg gcccccaggt gttggaagag taacggttgt
      541 cgggccagcg gcgtggttcg tgctcggcct ctagctgctc catgtcgcag tacagctcca
      601 cgaagcactc ctcctccacg atgcgcacgt aggaggcgat gtttccgccg atgccgtggc
      661 gcgcagggcc ccagcctagc cagcgtccgt gccttccaag gtggtcgagg gcgacccgca
      721 tctggccgat gtccaccacg tcgaaggcga gatggtgcag gtggctgtag cccttgtcca
      781 cgagggccat cacgtggtgg tcgccgttta tgtggaacca cactccgccg tcgccgagcc
      841 agtccgtcac ccgcatgccg agcacccgcg tgtagaactc cacaccgctc gagatgtccc
      901 cggtcaggaa gttgacgtgc cccagcttgc gaggatgccc gacaggctgg tccccggcag
      961 gcctggcgtg cgccgtccac cgcgaccccg gctccctgta gggagagacg atcacccggt
     1021 tcccctctgg gtcggccacc tccagaccgc cctcacgctc gctgtagtcg acgccgaggt
     1081 tctcgaaatg acgcccggcg tcgtctggcg tgcacgagcg attcagctcg taggccacgt
     1141 gcccgatacc cggggcttcg gcggcgacca gctccaggca gaacggctcg tcgtcgcagg
     1201 cgaggcggga cttccggtcg tcacgttcgc gctccacgag gccgaactgg ttcgaccagc
     1261 ggctggttgc atcgtcgaga tctgcgacgc gcaggctcac gtgggcgatg cggcggagcg
     1321 agatcaccgg accacccgca ctggcatgcg cttgatgccg ttgaagaagt tggacctcag
     1381 gcgttcggga gggcccgtga gctcgatgcc gtcgacgcgt ttcagcatct cctcgaaggc
     1441 aattcggacc tccagccgcg ccaggtgggc gccgaggcag tggtggatgc caccagggcc
     1501 aaaagccatg tgcttgttcg gctcgcggcc cacgtcgaag gcgtcggggt tatcgaagac
     1561 cgtctcgtcg cggttgcctg agacgagcca ggtcgtgacc ttggctccgg ccgggatctc
     1621 ggtgtcggcc agcaccgtgt ctctggtcgt ggtgcgccgg aagtagtgga cggccgtggc
     1681 ccagcgcagc atctcgtcgg cggccggctt gaacag
//
LOCUS       DBNGVD010000317         4911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4080707, whole genome shotgun sequence.
ACCESSION   DBNGVD010000317 DBNGVD010000000
VERSION     DBNGVD010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4080707"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="AC16CM_08470"
     CDS             complement(<1..1425)
                     /locus_tag="AC16CM_08470"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3139906.1"
                     /translation="MEVDRNRLADIELTDNALAVLKKRYLKKNEKGEAVEEPIEMFRR
                     VASNIAEGEFRFKEGEEAERLYQESRERFLQMMLSRKFMPNSPTLMNAGRELQQLSAC
                     FVLPVEDSIDGIYDTLKHQAIIHKSGGGTGFGFSRLRPKNDLVKSTMGVSSGPVSFMA
                     IYDASTDKIKQGGTRRGANMGILRVDHPDVEEFIDCKRDNDQINNFNISVAITDEFMK
                     AVEADADFDLRNPRDGVVIKTVRAKDLFRKIVEGAWLNGEPGVVFIDKINDDNPTPQF
                     EIESTNPCSEAHLPPYDSCNLGSINLERFYIEETGEVDWDELRETVHTAIRFLDNVIE
                     QNNYPLEEIDRMSRGNRRIGLGVMGFADLLIKLGITYDSDEGLAFAERMMKFVDDEAW
                     EESRALADERGVMPNFEGSRHEARGDRVRNATVTTIAPTGTISMIAGCSGGIEPLFAV
                     AFMRRQADMEMPDVNPEFVKLARER"
     gene            complement(1514..1990)
                     /gene="nrdR"
                     /locus_tag="AC16CM_08475"
     CDS             complement(1514..1990)
                     /gene="nrdR"
                     /locus_tag="AC16CM_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3139907.1"
                     /translation="MQCPYCDFEDTKVIDSRLSETKDAVRRRRECLSGACGKRFTTYE
                     RREPLRLMVIKRDGGKEPFDREKLRAGLAKACAKQQVTDEQVEFIVDEIEADLRERRR
                     HEVTSRRLGDMVLMRLRRMDMVAYLRFASVYRQYTDVDQFRTELVRLAGSGMGSTR"
     gene            complement(2356..2844)
                     /locus_tag="AC16CM_08480"
     CDS             complement(2356..2844)
                     /locus_tag="AC16CM_08480"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3139908.1"
                     /translation="MAERPEQQFSETDLAMMRRALCVAEEAAEKGEVPVGCVVARGGE
                     ILAVAANEREATGDPTAHAELLAVRRASAALGGWRLTGCTLYATLEPCPMCAGAAHAA
                     RLSRLVFAAPDPKAGYAGTLHNTPADTRLNHTLVVQGGLLAENAASLLRDFFRNRRRK
                     PG"
     gene            complement(2903..3670)
                     /locus_tag="AC16CM_08485"
     CDS             complement(2903..3670)
                     /locus_tag="AC16CM_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139909.1"
                     /translation="MLVHLPDRWGTSRVWRSWAADEEQADDLIEETAGEARAAGRRRV
                     LWHTGDLVAPPFMDECLTRHGFRKNEDLKVLAFVLGNGQEQQLPRMRDPEYLFVELVR
                     CAGALDEALRVDDEVFGSPSPTGGEFAEHAGELEELRRRERGGRTDDGASVAMRFVAH
                     MNSPPGCERESPLCTVAAAGAQIVGETLRLWGAGTRRAFRGRGAYGALVLERCRVGRH
                     FGTTLALAKANVATSAPILERAGFYPVGVERRHVLEI"
     gene            complement(4127..>4911)
                     /locus_tag="AC16CM_08490"
     CDS             complement(4127..>4911)
                     /locus_tag="AC16CM_08490"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3139910.1"
                     /translation="NMNRPEEMAYAEEHGIPVPTTKESPYSVDENLWGRSIEAGPLED
                     PDHEPTEDVFELTTSPENAPDEPQYVEVGFEEGLPISLDGEDLQLIDLIAELNAVAGA
                     NGVGRIDMIEDRLVGIKSREIYETPAALAIVAAHRELEALTLTRDVLRFKTTVEQRYA
                     ELAYEGLWFTPLKSALDAFITETQKTVTGSVRLKLYKGNATVVGRTAPNALYSKDLAT
                     YDPGSTFDEAAAAGFIALWGLSARQWASVNGGSEYSPAPSQH"
BASE COUNT          858 a         1601 c         1448 g         1004 t
ORIGIN      
        1 gcgctccctg gcgagcttga cgaactcggg gttcacgtct ggcatctcca tgtcggcctg
       61 tcggcgcatg aacgccacgg cgaagagcgg ctcgatgccg cccgagcagc cggcgatcat
      121 ggagatggtg cccgtgggtg caatggttgt gacggtcgcg ttgcggaccc tgtcacccct
      181 tgcctcgtgg cgcgagccct cgaagttggg catgacgccg cgctcgtcgg cgagggccct
      241 cgactcctcc caggcctcgt cgtccacgaa cttcatcatc cgctcggcga aggccagacc
      301 ctcgtcggag tcgtaggtaa tgccgagctt tatgagtaga tcggcgaagc ccatgacccc
      361 gagcccgata cgcctgttgc cccggctcat ccggtcgatc tcttccagcg ggtagttgtt
      421 ctgctcgatc acgttgtcca ggaagcggat ggcggtgtgt accgtctccc tcaactcgtc
      481 ccagtcgacc tcgcccgtct cctctatata gaagcgctcc aggttgatgc tcccgaggtt
      541 gcacgagtcg taaggcggca gatgcgcctc cgaacagggg ttggtgctct cgatctcgaa
      601 ctgcggcgtc gggttgtcgt cgttgatctt gtctatgaac acgacgccag gctcaccgtt
      661 gagccaggca ccctccacga tcttgcggaa gaggtccttc gccctgacgg tcttgatgac
      721 cacaccgtcg cgggggttcc tgagatcgaa gtccgcgtcg gcctcgacgg ccttcatgaa
      781 ctcgtcggtg atcgcaacgg agatgttgaa gttgttgatc tggtcgttgt cgcgcttgca
      841 gtcgatgaac tcctcgacgt ccgggtggtc caccctgagg atgcccatgt tggccccccg
      901 ccgcgtgccg ccctgcttga tcttgtccgt cgaggcgtcg tagatggcca tgaacgaaac
      961 gggtccgctc gacacgccca tcgtgctctt gaccagatcg ttcttgggac gcaacctgga
     1021 gaacccgaag cccgtgcccc caccgctctt gtggatgatg gcctggtgct tgagggtgtc
     1081 gtagatgccg tcgatagagt cttcgaccgg caacacgaag cacgcgctga gctgctgcaa
     1141 ctcacgtccg gcgttcatga gcgtcggcga gttgggcatg aacttccggg agagcatcat
     1201 ctggaggaac ctctccctgg actcctgata gagcctctcg gcctcctcac cctccttgaa
     1261 gcggaactcg ccctcggcga tgttcgacgc gacccgccgg aacatctcta taggctcctc
     1321 gacggcctca cccttctcgt tcttcttgag gtagcgcttc ttgagaacgg caagcgcgtt
     1381 gtcggtgagt tctatgtctg caagcctgtt tcgatccact tccatactaa tcctccctat
     1441 tctaccgctt gtttcttcga ttccatcacc ggaatggccc gccctacccc gtttcggcgg
     1501 gccccggtcc ctactacctg gtgctgccca tacccgagcc ggcgaggcgc acgagctcgg
     1561 tacggaactg gtccacgtcc gtgtattgcc tgtagacgga cgcgaaccgc aggtacgcga
     1621 ccatatccat cctcctgaga cgcatcaaga ccatgtcccc gagacgcctc gaggtcacct
     1681 cgtgccgcct tcgctcgcgc aggtcggcct cgatctcgtc gacgataaac tccacctgct
     1741 cgtccgtgac ctgctgcttg gcacaggcct tggccagccc agcccgcaac ttctcccggt
     1801 cgaacggctc cttgccaccg tcgcgcttga tcaccatgag cctcaacggc tcacgcctct
     1861 cataggtggt gaacctcttg ccacacgccc ccgagagaca ctcccgacgt cgcctgacag
     1921 cgtcctttgt ctcggaaagc cgagaatcta tgaccttcgt atcctcgaaa tcacagtacg
     1981 gacactgcat ctctaagccc caatcgctcc aacaccatat ctggtactgc gaaaacctag
     2041 cacactatat tttatggatg caataccccc aaaatggacc ttaacttcag gttaaattga
     2101 cgctaaaccc ccgaatgtcc cgttcgcacg cggaatacgc ccgaaacacc ccgccccgag
     2161 aatttttcaa agtttttttc atatcgaccc gtttacaggg aatgtcccgc aaattgcgta
     2221 ccgttatgtt caggtccaca gatgccctta tagtaacaat cgaggctaag ttatgtacac
     2281 caataaatct atacctcaag tataggtaca gtgggtgtct ggctttcttc cccgggatgg
     2341 agggggatcc gggctctatc cgggtttgcg gcggcgatta cggaagaagt cgcggagcag
     2401 cgaggcggcg ttttccgcga ggagcccccc ctggacgacg agggtgtggt tgaggcgggt
     2461 atcggccggg gtgttatgga gggtgccggc atagccggct ttggggtcgg gtgccgcaaa
     2521 gacgaggcgg gagagccggg cggcgtgggc ggcgccggca cacatggggc aaggttccag
     2581 ggtggcgtag agggtgcagc ccgtcaggcg ccagccgccg agggcggcgg aggcacgtcg
     2641 tacggccaga agctcggcgt gggcggtggg atctcccgtc gcctcgcgct cgttggcggc
     2701 gacggcgagg atctcgcctc ccctggcgac cacgcacccg acggggacct cccccttctc
     2761 ggccgcctct tcggcaacac agagagcgcg gcgcatcatc gcgaggtcgg tctcggaaaa
     2821 ttgctgttct ggtctctctg ccaccaggcc gatgataaca gcgcgcggtt tgggcctgtc
     2881 gtgagcaatt aggccacagg tttcagatct ccagcacatg acggcgctcg acgccgacgg
     2941 gatagaaacc cgcgcgctcg agtatggggg ccgaggtggc gacgttggcc ttggcgagag
     3001 ccagcgtcgt gccgaaatgc ctcccgaccc tgcatctctc caggaccagc gcgccgtatg
     3061 cgccccggcc cctgaacgcc cgacgcgttc ctgctcccca caacctcagt gtctccccga
     3121 cgatctgggc cccggcggcg gccacggtgc agagcgggct ctctctttcg caccctggag
     3181 gagagttcat gtgtgctacg aacctcatcg cgaccgacgc accgtcatct gtccgcccgc
     3241 ctcgctcgcg acgtcgcaac tcctccagct ctccggcgtg ctcggcaaac tccccgcccg
     3301 tcggcgatgg tgaaccgaaa acctcgtcgt caacgcggag cgcctcatcc agtgccccag
     3361 cacaacgaac cagttccaca aacagatact ctggatctct catccggggc aactgctgct
     3421 cctgaccatt tcccagaacg aacgcaagga ccttcagatc ctcgttcttt cggaagccgt
     3481 gcctcgtaag acactcgtcc atgaacggtg gcgccacgag atctcccgtg tgccacagaa
     3541 ccctcctcct gccagcagcc cgggcttcgc cagccgtctc ctcgatgagg tcgtccgcct
     3601 gctcctcatc tgccgcccat gagcgccaca cccggctcgt gccccaacga tccggcagat
     3661 gtaccaacaa acgccgctcg tcctcgatac acctaccatc tcgcgggaca tgaacccaac
     3721 gccctgactc ccgcagtacg gccccctctg tccatctctc gcccacatga acagggtagc
     3781 ggagcgatgc cgggccggca gaagattcca gacctcaatg ctccagagct atccctatcc
     3841 gcccaccgtg cggccaggtg atggcccctt ccaggatatg gcgtcgttaa cgcgcggaag
     3901 accggccctt aagcgagccg tagccccccg cttgctcgag ggagtgaagc ccggcacacg
     3961 cccgcggccg gaaatcccga ccgcgaaccc ggcaccagca tgtactccag atttaccgtg
     4021 aacctttgat acaggtttat tcttctgtca gcgacgtgga ggcctgattc tacacctgat
     4081 tgtcctggaa acagcacttt tttgttctac agtatcaacc gttggtttag tgttgcgaag
     4141 gagcggggct gtactctgaa ccgccgttga cgctcgccca ctggcgggcg gagaggcccc
     4201 acagggcgat aaagccggcg gccgcggctt cgtcgaaggt gctgccgggg tcgtaggtgg
     4261 ccaggtcctt gctgtagagg gcgtttgggg cagtgcgccc cacgacggtg gcgttgccct
     4321 tgtagagctt gaggcggacg gagccggtca cggtcttctg tgtctccgtt atgaaagcat
     4381 caagggcaga tttcaggggg gtgaaccaca ggccttcgta ggcgagctcg gcgtaacgtt
     4441 gttccacggt ggtcttgaag cgcaggacgt ctctggtcag ggtcagggct tcgagctccc
     4501 ggtgggctgc gacgatagcg agggcagcgg gggtttcgta gatctcgcgg ctcttgatgc
     4561 cgacgagtcg gtcttcgatc atgtctatgc ggcccacgcc gttggcgcca gcgacggcgt
     4621 tgagctcggc tatgaggtct atcagctgca ggtcttcccc atccaggctg atcggaaggc
     4681 cctcttcgaa gccaacctcg acgtactgcg gttcgtctgg ggcgttctcg ggggacgtgg
     4741 tcagctcgaa cacgtcctca gtgggttcgt ggtcggggtc ctctagaggg ccggcctcga
     4801 tggagcggcc ccagaggttc tcatccacgc tatagggtga ctcctttgtg gtcggcacgg
     4861 ggataccgtg ctcctcggcg taggccatct cctcggggcg gttcatgttc c
//
LOCUS       DBNGVD010000318         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2987837, whole genome shotgun sequence.
ACCESSION   DBNGVD010000318 DBNGVD010000000
VERSION     DBNGVD010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2987837"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="AC16CM_08495"
     CDS             <1..887
                     /locus_tag="AC16CM_08495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR3139911.1"
                     /translation="AYRVFVLFSLILAIGYLVWTVEELRLRPGWAAVGVVMLLLSSPL
                     LSTLALGQVYPVLALGLVASWLADRRGRLEISGAALGLVVALKPSLLPVLLWPLVRRR
                     WRAVGAAVAAGVLATLIGVLVLGPGTTLDYVGVLRDRSVSPYWDNASLPAAAARLFTE
                     NSYAQNVATLPWMVTLGYALGIGTILLSALRVRHGPEVGLWVLVAASLLASPIAWHNY
                     LVLLGPGVLVLFARGMAAPAFLLLALQAIPAQWPLIWNQKGTVVASLALTLYMFVLIA
                     HWLALLAATREPSIVEDG"
     gene            963..1859
                     /locus_tag="AC16CM_08500"
     CDS             963..1859
                     /locus_tag="AC16CM_08500"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3139912.1"
                     /translation="MSQTIMQRIVEALPVLDRVSDALQPGVQKAVDAGGTTARNVLDG
                     VWFEAPLHPVMTDVPIGAWTTALVFDGLDLATGKKPIRHAADAALLFGTLGAFGAAAT
                     GLSDWRYLSGGSRRMGVAHGLLNTVGLAFSIASLIQRATGRRNAARLTFLTGYSINGM
                     AAHLGGELSYHYGLRVDRNVFEKPGPDDFTPVLDEQDLGPDELRRVEVEGVGVLLSRS
                     SGGQICAIAATCNHFGGPLEQGEREDDTVICPRHNSRFDLCSGQPIDGPAVFPQSRYE
                     TRTRDGKIEVKAAAENIQRIVR"
     gene            complement(2191..2811)
                     /locus_tag="AC16CM_08505"
     CDS             complement(2191..2811)
                     /locus_tag="AC16CM_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3139913.1"
                     /translation="MRLLYPTLGALVILAVLSVVIGRQNRDLLRVRAYRDFFLRLIPP
                     LSTGVFIAGLPFVMNLTTVENYGSVLLVYIAGAAVLTSLMTRAVTPDERRATAAFRKG
                     EYEKAADYYDEISTRRPLPRHFSALGASLEASGNPVAAIEATDRAIKLDPKLGIAYYN
                     RASALASLDEKARARGDLQKVFQVDSNRNLRRAAAEALESLEHTSP"
     gene            complement(2808..3410)
                     /locus_tag="AC16CM_08510"
     CDS             complement(2808..3410)
                     /locus_tag="AC16CM_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139914.1"
                     /translation="MGSGAITIVLIFLLLFVSWQTIYAANRDLARAKAWGDLLRRALP
                     FFALLLMVLSLFVIQSFGQGWTLLAWMAVAGVAIIGASLLSITSLERNANRAFRSGDY
                     ARAVELYEKLVQEKPLARNKAFLGAALGASERNEESVDLSTQAIEQDPEYGLAYYNRA
                     LVLQRMGKKSRVVKDLNRALEVDLPRRFRSAARGMLEEMS"
     gene            complement(3418..>3804)
                     /locus_tag="AC16CM_08515"
     CDS             complement(3418..>3804)
                     /locus_tag="AC16CM_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HIR3139915.1"
                     /translation="DGIPDLTDDGPLRFSSFAEVSGAYEISEPEELAALDQHHMWTSE
                     YAESRFKWRPKKPLTVLVLRTYLLPEAVELPYMDEYGGCKSWIELQQTIPTEGARPAL
                     SEEEFQALVTPALGVLRTLEPAPVGA"
BASE COUNT          599 a         1189 c         1333 g          683 t
ORIGIN      
        1 ccgcgtacag ggtgttcgtg ttgttctcgc tgatcttggc catcggttac ctggtgtgga
       61 ccgtggagga actgcgttta cggccggggt gggcggcggt gggggtggtg atgctccttc
      121 tctcctctcc gctgctgtcg acgctggcgc tcgggcaggt ctaccccgtg ctcgcgctcg
      181 gactcgtggc ttcgtggctg gcggataggc gggggaggct ggaaatctcg ggcgcggcgc
      241 tcggtctggt ggtggcgttg aagccttcac tactgcccgt gcttctctgg cccctcgtgc
      301 ggcgcagatg gagggccgta ggggccgcgg tcgccgctgg ggtactggcc acgctcattg
      361 gcgtgctcgt gctcgggccc gggacgacgt tggattacgt cggggtgttg agggaccgtt
      421 ccgtcagccc gtactgggac aacgcctcct tgccagcagc ggcggcacga ttgttcacgg
      481 agaactcgta tgcccagaac gtggcgacgc ttccctggat ggttaccctc ggttacgcgc
      541 tcgggatcgg gaccatcttg ttgagcgccc tgcgggtcag gcacgggccg gaggtgggac
      601 tctgggtgct cgtcgccgcc tcgcttctcg cctcgcccat cgcctggcac aactacctcg
      661 tgttgctcgg tcccggcgtc ctggtgctct ttgcgcgggg tatggccgcc ccggctttcc
      721 tgctgcttgc cctgcaggcc atcccggcgc agtggcccct catctggaac cagaagggca
      781 cggtggtggc atcgctggcg ttgacgttgt atatgttcgt cctgatcgca cactggctcg
      841 cccttctcgc agcgaccaga gagccgtcca tcgtcgagga cgggtagcgg ttcatgggcc
      901 ggagtggcat cgttcccgtt cgggtatagg gatcagtacc caccagcgga aaggcggaag
      961 agatgtcaca gaccatcatg caacgcatcg tcgaggcgtt gcccgtcctg gacagggtct
     1021 cagacgcctt gcagcccggg gtgcagaagg ccgtagacgc gggcggcacg acggcgcgca
     1081 acgtactaga cggcgtctgg ttcgaggcgc cgttgcaccc cgtgatgacc gacgttccga
     1141 tcggggcctg gaccacggcg ctcgtcttcg acggcctcga cctcgcgacg ggcaagaagc
     1201 ccatacggca cgcggctgac gctgccctcc tgttcggcac cctgggtgcc ttcggggcgg
     1261 cggcgaccgg gctctcggac tggcggtacc tgagcggggg ctccagaagg atgggggtcg
     1321 cccacggtct actgaacacg gtcgggctcg cgttcagcat cgcctcgctc attcagaggg
     1381 ccacgggacg caggaacgcg gcgcgactca cctttctgac cggctactcg atcaacggca
     1441 tggcggccca cctcggcggg gagctctcgt accactacgg cctgcgcgtg gacaggaacg
     1501 tcttcgagaa accaggcccc gacgacttca cccccgtcct ggacgaacaa gacctggggc
     1561 ccgacgagct acgccgggtc gaggtcgaag gcgtgggcgt gctactcagc cgctccagtg
     1621 gcggccagat ctgcgccatc gcggccacct gcaaccactt cggcggcccg ctggagcagg
     1681 gcgagcgcga ggacgacacc gtgatctgcc cccggcacaa ctcacgcttc gacctgtgca
     1741 gcggccagcc catagacggc cccgccgtct tcccccagtc ccgatacgag acgcgaacca
     1801 gggacgggaa gatagaggtc aaggccgccg cagagaacat ccagaggata gtcaggtaga
     1861 gcacgctccg gctaacgcct gagcttgtca gcacgccggc tccgcttcgc tccgccggct
     1921 tgtcagcacg ctcgggcttt cgccctcgct tgtcggcaac gcttcggctt cgcctcgctt
     1981 tcggccagtc gtctctctcc gtaacggtgg cttcgacata tagggctttt ccgggcagcg
     2041 aatcgttaga gcggaaaggt gaaggccgca gcgggatctg gccgacagcg agacccgaag
     2101 ggtcgaagcg ttgctgacaa gcgacagccg ccaggctgga gcgtgctgac aagcggaggc
     2161 gttagccgga gcgtgctgac acggagcccg tcagggcgac gtgtgctcca ggctttccag
     2221 ggcttcggcg gcggcgcggc ggagattgcg gttcgagtct acctggaaga ccttttgaag
     2281 gtcgccgcgg gccctggctt tctcgtcgag ggaggccagg gccgaggccc ggttgtagta
     2341 ggcgatgcca agcttcgggt cgagctttat ggcacgatcg gtggcttcta tggcggcgac
     2401 ggggttgccc gaggcttcca ggctcgcccc cagggcggag aagtggcggg ggagtggcct
     2461 gcgggtggag atctcgtcgt agtagtcggc tgccttttcg tactcgcctt tgcggaaggc
     2521 ggccgtggcc cggcgttcgt cgggggtgac ggcgcgggtc ataagcgacg tcaggactgc
     2581 ggctccggct atgtagacga gcaggacgct gccgtagttc tcgacggtcg tgaggttcat
     2641 cacgaagggg aggccggcga taaagacgcc ggtggagaga ggcggtatga ggcgcaggaa
     2701 gaagtcgcgg taggcccgca cacgcagcag gtctctgttc tggcggccga tcacgacgct
     2761 caggaccgcg aggatgacga gcgcgcccag agtggggtag aggagcctca agacatctct
     2821 tccagcatcc ccctggctgc cgaccggaag cggcggggaa ggtccacctc cagcgccctg
     2881 ttgaggtcct tgacgaccct gctcttcttg cccatccgtt gcaagacgag ggcgcggttg
     2941 tagtaggcga ggccgtactc ggggtcctgc tcgattgcct gcgtggagag gtccacggat
     3001 tcctcgttac gctcggaggc cccgagggcg gcccccagaa aagccttgtt gcgggcgagg
     3061 ggcttctcct ggacgagctt ctcgtagagc tccaccgccc tcgcgtagtc gccgcttcgg
     3121 aaggccctgt ttgcgttgcg ctccagggac gtgatggaga ggaggctggc ccctattatt
     3181 gcgacgcctg ccaccgccat ccaggccagg agcgtccagc cctgcccgaa gctctggatc
     3241 acgaacaggc taagcaccat cagtagcagg gcgaagaacg gcagggcgcg gcgtagcagg
     3301 tcaccccacg ctttcgccct cgccaggtcg cggttggcgg cgtatatggt ctgccagctc
     3361 acgaagagga gaaggaagat cagaactatg gttatcgctc ccgagcccat tgctacctca
     3421 ggcgccgacc ggcgcgggtt cgagggtccg aagcaccccg agtgcgggcg tgacgagggc
     3481 ctggaactcc tcctccgaga gcgcgggtct ggccccctcg gtagggatgg tctgctggag
     3541 ctcgatccag gacttgcagc ccccgtactc gtccatgtag ggaagctcga cggcctcggg
     3601 cagcaggtac gtgcgcaaga cgaggaccgt cagcggcttt ttggggcgcc acttgaagcg
     3661 gctctccgcg tactccgagg tccacatgtg gtgctggtcg agggccgcga gctcctccgg
     3721 ctcggagatc tcgtaggccc cggagacctc ggcgaacgag gagaagcgca ggggaccatc
     3781 atcggtgaga tcagggatcc catc
//
LOCUS       DBNGVD010000319         3647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1894385, whole genome shotgun sequence.
ACCESSION   DBNGVD010000319 DBNGVD010000000
VERSION     DBNGVD010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1894385"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..893)
                     /locus_tag="AC16CM_08520"
     CDS             complement(252..893)
                     /locus_tag="AC16CM_08520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3139916.1"
                     /translation="MFLFLFQSQPSVAVAFLLGLVVGITVHEASHAASAYLLGDDTAY
                     REGRVTLNPISHLDVMGSLMLLMAGFGWGKPTPVVPSKLRGGVFGPVAVALAGPVSNL
                     VIVAICAALYLLPPFQDPGYLYILVLMVAFTNALLFVFNLIPIPPLDGSKILFPFLPR
                     ALDGFVFFMNQYGPIILLGLILVSFVTNLPIFNILLAPVAPILNFFGLPPVGF"
     gene            complement(1253..2062)
                     /locus_tag="AC16CM_08525"
     CDS             complement(1253..2062)
                     /locus_tag="AC16CM_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139917.1"
                     /translation="MGKPGRKRTPRKPSHPGSTNGRAGSPAGNFVELREARKAKRGEV
                     YKKRRLLAALLLTLGALTLILAVFVQTSASDTGDGAVPIDPNNAGPDTVLAEASGVGI
                     STPIRPASLSGLGYHAEGESIIAMEPRGKNLSANALVGLFSRGETPEQINYYVMDASG
                     REGPQTGALDVGAPTGTTVYAPVTGTVTAIRPDPMVDDANVVEIKPDANPNVRVNVSL
                     VQSDGNAGVNDDVTAGITELGTVADSAQVLDPQLSSYTHDTGNHVTVSVSG"
     gene            2211..3383
                     /locus_tag="AC16CM_08530"
     CDS             2211..3383
                     /locus_tag="AC16CM_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3139918.1"
                     /translation="MPTRKLVVSTYLQYIALALGVVLLVAFVRQVGGVLLTFLAAGVL
                     AYVLNPIVRRLEEMRVPRIIAVLGVFVVLFLVVGVTLLLIIVPSIGQVQIIIRSPQDL
                     VDQAVALLNRVQDIPYVGRYVRELNQDRILQLVQDNAPSAQQVANVTTGVIGGVFGVF
                     GTIFNLFLMVLISIYLLLEREKITGAMLNTIPETVRDQSLELFHAVEGSLIRYLRGQL
                     LLCAIMGVLGWAIMFFTVGEYALAVGAWVAATELIPVLGAFLGAIPAVAIALFVPQGG
                     FFEAVLVAFLFLIAQQLEGNLLVPRIQGGSVGVHPLVVLFATLAGTALYGILGAIFAV
                     PLVAIIAATIRYLRGTLLYERWDKPPVRAAEPEQDGQVRASPSMAAGTEDEAEQAR"
     gene            3380..>3647
                     /locus_tag="AC16CM_08535"
     CDS             3380..>3647
                     /locus_tag="AC16CM_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3139919.1"
                     /translation="MTIPHQEPDKPTFREASRGALLFLLRPLVRWLSVMRVRPDTLTV
                     VGWTLALCAAVLFGLGHARVAGLVMLTGGVFDALDGAVARETNRM"
BASE COUNT          611 a         1117 c         1229 g          690 t
ORIGIN      
        1 gctccggctt cgcctccgct tgtcagcacg ttacggccct tcgggcctgc acttgtcagc
       61 acgctccagc ctggcggctg tcgcttgtcg gcaacgcttc gcccggctcc gcctgggctc
      121 gctttcagct agatcccgct tcggcctgca cccttccgct ctgaagattc gctgcccggg
      181 aaagccctat gtcgaagcca ccgttacgaa tcgagacgac cagccgaaag cgaggcgaag
      241 ccggagcgtg ctcagaagcc tactggtggc agtccgaaga agttcaggat gggggctacg
      301 ggtgccagaa ggatgttgaa gatagggagg ttggtgacga aggagaccag gatcaggccg
      361 agcagaatta ttggcccgta ctggttcatg aagaacacga agccgtccag ggctcggggc
      421 aggaatggga agagtatctt cgagccgtcg agcgggggaa ttgggatcag gttgaacaca
      481 aagagcaggg cgttggtaaa ggcgaccatg aggacgagga tgtacaggta gccgggatcc
      541 tggaagggtg gcaacaggta cagggcggcg cagatggcca cgatcacgag gttggatacg
      601 ggtccggcga gggcgacggc caccggcccg aacacccctc ccctgagttt ggagggcacc
      661 accggcgtcg gtttgcccca tccgaagccc gccatcagga gcatcaggct ccccatcacg
      721 tcgagatgtg agatgggatt gagggtcacc cttccttcac ggtaagcggt atcgtcccca
      781 agcagataag ccgaagccgc gtgggaagcc tcgtggaccg tgatccccac gaccagcccc
      841 agcaagaatg ccacggcaac agagggttgc gactgaaaaa gaaacaaaaa cattacgaca
      901 tgatacacgc ttcggcccgc gggctacgcc gcacgcttcg gccaacgcct ccgcttgtca
      961 gcacgctcca gccttgcggc tgtcgcctcg ctttcggctg atcgtctcga ttcgcatcgg
     1021 tggcttcgtc tacccacctt ttccgggtat cgagtcttta gaggggtagg gtgcgggccg
     1081 cagagacatc tggctgaaag cgagcagggc gaagccgggc gaagagttgc tgacaagcaa
     1141 gggcgttagc ccgagcgcgc tgacaaacgg aggcgttggc cgaagcgtgc tgacaagcgc
     1201 aggccgaagg ccgaagcgtg ctgcgagggc gttagcccga ggccggctcg cctcagccgc
     1261 ttacgctgac ggtgacgtgg ttgccggtgt cgtgggtgta ggaggagagc tgggggtcaa
     1321 ggacctgggc cgagtcggcc accgtgccaa gctcggttat gccggcggta acgtcgtcgt
     1381 tgacgccggc gttgccgtcg ctctgcacga gcgagacgtt gacgcggacg ttcgggttgg
     1441 cgtctggctt tatctccacg acgttggcat cgtcgaccat ggggtcggga cggatggccg
     1501 tgacggtgcc cgtcacgggg gcgtagacgg tggtgcccgt gggtgccccg acgtcgaggg
     1561 cgccggtctg gggtccctcc cttcccgaag cgtccatcac gtagtagttg atctgctccg
     1621 gcgtctcccc cctggagaag aggccgacca gcgcgttggc cgagaggttc ttgccccggg
     1681 gctccatcgc gatgatgctc tcaccctctg catggtagcc gagcccgctc aggctcgcgg
     1741 ggcggattgg ggtggagatt cccacgcccg aggcctcagc cagaaccgtg tcgggaccgg
     1801 cgttgttcgg gtcgattggg acggccccgt cgccggtgtc cgaggcgctg gtctggacga
     1861 acacggcgag gatcagcgtg agagcgccga gcgtcagcag cagggcagca aggaggcgcc
     1921 tcttcttgta gacctcgccc cgcttcgcct ttctagcttc gcgtagctcg acgaagttgc
     1981 cggcgggact tcccgcacga ccgttggtcg atcccggatg cgaaggcttc ctgggagtac
     2041 gcttgcgccc gggctttccc atccgctccc caacatgttg ggttctctct gcgtccaacc
     2101 ctctcatact gggcgaggat tatacagagg ggtacccgag gtgtaaactc gcgcaccgta
     2161 ctgtcgcatg ctggtatatt ttgtatcgta gagctcggat cggagcgggg ttgcccacca
     2221 gaaaactggt cgtatcaacg tacctgcagt acatcgcgct tgccctaggc gtagtactcc
     2281 tggtggcctt cgtgcgccag gtcggcggcg tgttgcttac cttcctggcc gctggcgtcc
     2341 tcgcctacgt cctgaacccg atcgtgcgtc gcctcgaaga gatgcgtgtc ccgcggatca
     2401 tagccgtcct cggggtgttc gtggtcctgt tcctggtcgt cggggtgacc ttgctcctga
     2461 tcatcgtccc ctcgataggc caggtgcaga tcataatccg tagtccgcag gacctcgtag
     2521 atcaggccgt cgcgctgctt aaccgggtcc aggacatccc gtacgtcggc cggtacgtga
     2581 gagagctgaa ccaggaccgg atcctgcagc tcgtgcagga caacgccccg tcggcccaac
     2641 aggtggcgaa cgtgaccacg ggcgtgatcg gcggggtgtt cggggtcttc gggaccatct
     2701 tcaacctctt tctgatggtg ctgatctcca tctacctgct gctggagcgg gagaagatca
     2761 ccggcgcgat gctcaacacc atcccggaga cggtacgcga ccagagcctg gagctcttcc
     2821 acgcggtgga ggggtccctg atcaggtacc tgaggggaca gctgctcctc tgcgcgatca
     2881 tgggcgtcct gggatgggcc atcatgttct ttaccgtggg agagtacgcc ctcgccgtgg
     2941 gggcctgggt ggcggccacg gagctgatcc ccgtgctcgg ggctttcctg ggggccatcc
     3001 cggcggtcgc catagcgctg ttcgtccccc agggcgggtt cttcgaggcc gtgctggtgg
     3061 ccttcttgtt cctgatcgcc cagcagctcg agggcaacct gctcgtgccg aggatacagg
     3121 gcggctcggt tggcgttcac ccgctcgtag tactcttcgc gacgctagct ggtactgccc
     3181 tatacgggat acttggcgcg atcttcgcgg tccccttggt cgccatcatc gccgcgacga
     3241 tccgctacct gcgggggacc ctgctctacg agcgctggga taaaccgccg gtgagggccg
     3301 ccgaaccaga gcaggatgga caggtaaggg cctcaccctc gatggcggcc ggcaccgagg
     3361 atgaggcgga acaggcacgg tgaccattcc ccaccaggaa ccggacaagc ctactttcag
     3421 ggaggcgtcc aggggggcgc ttctcttcct gctgcgtccg ctcgtcaggt ggctctccgt
     3481 gatgcgcgtg cgcccggata cgctcacggt cgtgggatgg accctggcgc tgtgcgccgc
     3541 cgtcctgttc ggcctcggcc acgcgagggt cgccggcctg gtgatgctca cggggggcgt
     3601 tttcgatgcg ctcgacggcg ccgtcgcccg cgagacgaac cggatga
//
LOCUS       DBNGVD010000320        18474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2805295, whole genome shotgun sequence.
ACCESSION   DBNGVD010000320 DBNGVD010000000
VERSION     DBNGVD010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18474
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2805295"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16CM_08540"
     CDS             complement(<1..670)
                     /locus_tag="AC16CM_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139920.1"
                     /translation="MIDNSDTVDARSNSEAPTSGVTESVVVTAVRALTQASQTLTAAA
                     TQMMQTVQGLPDGTSAKDIAQFPAGGVLPAASSVPQINTWEDDPFSEATPTTNPVSPD
                     PVVVDVTTINAPLLRFAIRDPQPLPARYPPGTPDFRYWVAAEALTRGINFWMPLLPTG
                     TRWSAASSPLPVNLVVGQLLNAQYRRFDGLRFFQNTVNGIPVYSGESPDVVCHELGHA
                     ILDAL"
     gene            complement(1504..2964)
                     /locus_tag="AC16CM_08545"
     CDS             complement(1504..2964)
                     /locus_tag="AC16CM_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR3139921.1"
                     /translation="MKTFERIKRVVHTWANNTLNSAAYVLSGGTKTLHEGRYHRSTGR
                     WTNWNNTFSARPQRFETPESEAAICEMVGLSKSVRVIGGGHTFNPSPLSVETMLSLDK
                     YNKILAVDTENRVVRVQAGVRLRDLMQELGERGLALPVLGSTNAQSLAGLIATDLHGT
                     GRDHGFLSEQVLSLRIVNAAGKAETFRRDSDVFRAAFGGLGSCGVVTEVELECVPDFN
                     LGKGIRVVRRDWVERNIDAILEENDHTSFYYIGGGGVERVRMNLWNRTVLEPPATIKL
                     RKMRNELIDMLFSGYLLGLARALKLTGPTAALGLLFFKLTMDGRSDVYSSAEGFARKL
                     YYRHDEIEFGIPYEAHQECMKELEAMLREKGHVTIIEVRFTPDKSNGLLGPGAGRRTC
                     YIELAPSLSLDPAEMFDEAEKIFLKYGGHPHLGKATKVTSAGMETMHGERFVVFQRAR
                     ERQDPEGKFLNDFAAQVFSEGAAAPASGHVDQLLHT"
     regulatory      complement(3198..3278)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3775..3924
                     /locus_tag="AC16CM_08550"
                     /pseudo
     CDS             3775..3924
                     /locus_tag="AC16CM_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4309..4578)
                     /locus_tag="AC16CM_08555"
     CDS             complement(4309..4578)
                     /locus_tag="AC16CM_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR3139922.1"
                     /translation="MAPRTRRAISSREADRQTLREAFEFCRNGDVLVVARLDRLGRSL
                     RELIGLVGELEERGVGFRGLDESLRSASRRRGLDRDRPDLTTNPT"
     gene            complement(4777..5394)
                     /locus_tag="AC16CM_08560"
     CDS             complement(4777..5394)
                     /locus_tag="AC16CM_08560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139923.1"
                     /translation="MRGTLAGKTVAVLGGSSPHGAAAARELGRQGANVALGGRDRQRL
                     EELEEEIRAHGGEALVVGVHLAKRHHPAHLVAAAVETFGGLDVLLYMASAAAPPLGSL
                     NLDAWERSLDVNVRGFVYCLAAALPAMRERGGGHVVMAGVEDSGGTRDPLLRAWRAAV
                     GTILEELPRELCTDGIRAAEVELSARQAGDPEECARTIVRALIDP"
     gene            complement(5391..5918)
                     /locus_tag="AC16CM_08565"
     CDS             complement(5391..5918)
                     /locus_tag="AC16CM_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139924.1"
                     /translation="MFHGLSEKTNWRAVIVGWAVAIAAGIVLNLLFEAAHVLLFGGDV
                     MNPDNLKTALVTLSLLSGFLAHFAGGYVAGRKARAHGGLQGVMVAVLGFLFVVAAALI
                     TSAIVVATAGLFLVEGGVALPALTLGLAGGALIAGLALLVLNVLGGFFGGKLGEWETG
                     PVEGSGGAGPLAGGR"
     gene            complement(5940..6710)
                     /gene="narI"
                     /locus_tag="AC16CM_08570"
     CDS             complement(5940..6710)
                     /gene="narI"
                     /locus_tag="AC16CM_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR3139925.1"
                     /translation="MNAWHQFLWVIFPYLSLVTFVLGHVYRYRYDQYGWTPKSSQLLE
                     RRLLMWGVLLFHWGFFFVFGGHVMGLLVPLGVYRAIGVSDHNYHLLSLWVGAPVGVVA
                     MVGILLLNIRRWFIPRIRNNTESIRFLTDGLLLVVIFLGLAATVGYRVYASQYELHGE
                     FEYRDSVGAWFRSLFLFRPDAALMVGAPLIFQLHTLSAFVLFALWPFSSLVHVFSFPL
                     GYLRRSYVQYRSSNPQKTLARARAGKGPAKAEQSSRVE"
     gene            complement(6707..7375)
                     /gene="narJ"
                     /locus_tag="AC16CM_08575"
     CDS             complement(6707..7375)
                     /gene="narJ"
                     /locus_tag="AC16CM_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HIR3139926.1"
                     /translation="MIFKLLSILLRYPDDGLMEHRAEISEAARGLPDSPAKDNILAFL
                     AHWESEPASRLQASYTGAFDFKRRGCLYLSYYRYGDQRARGQVLADMKAAYARAGYPL
                     DTTELPDYLPLVLEFASEVLEEGLPMLAENRAAIEVIRRSLRHDNSPYSHLVEALTLF
                     LPDLDEETAAEMQKLIVQGPPQESVGLEPYAADVPPGNPFGNEGSLGGAAPTGHVAFH
                     GRKR"
     gene            complement(7372..9096)
                     /gene="narH"
                     /locus_tag="AC16CM_08580"
     CDS             complement(7372..9096)
                     /gene="narH"
                     /locus_tag="AC16CM_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HIR3139927.1"
                     /translation="MRVQAQVAMVMNLDKCIGCHTCSVTCKNVWTSREGTEYQWWNNV
                     ETKPGVGYPKLYEDQERWKGGWALGKNGKLELKAGGKLRKLANIFFNPDLPSIDDFYE
                     PWTYDYETLTNAKLSEHQPVARPISQLSGEPVDLQWGPNWDDDLAGAPETGHLDPNIR
                     GELEESVRFEYEKVFMMYLPRICEHCLNPSCVASCPSGAMYKREEDGIVLVDQEQCRG
                     WRYCVSGCPYKKVYFNWDSGKAEKCTLCYPRIETGQPTICSETCVGRLRYIGLVLYDA
                     DGVEEAASVPDEKDLLEAQRSVFLNPNDPEVAEQARFDGIPDDWISAARRSPIYRLAI
                     EWGIALPLHPEYRTMPMVWYVPPLSPVMGFVEGNGAEADPDDVFPAIDELRIPIQYLA
                     NLLSAGDEGVVRRVLKKLAAMRGYMREKDLSGIGDAEIASAVGMEPGEIEDMYRLLAI
                     AKYEDRYVIPLAHKELAAELYEQRGTAGLDFAGGRTPAEVEHRNDAHACGLDFAGGPG
                     SCSSSAEAATLGGGRNGAFYAMKDELEKKARELGGKAQAPNVQLFTSKKDFAAFHLKG
                     RGPKGADA"
     gene            complement(9096..12782)
                     /locus_tag="AC16CM_08585"
     CDS             complement(9096..12782)
                     /locus_tag="AC16CM_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HIR3139928.1"
                     /translation="MAKSTNAFLRNLQYFRRGEKRSDGHAELSARDREWEYAYRQRWQ
                     HDKIVRSTHGVNCTGSCSWKVYVKDGIITWESQQTDYPSNGPDSPEYEPRGCPRGASF
                     SWYTYSPIRIKYPYVRGVLVEMYREALARIGDPVDAWASIVEDPEKARRYKAARGKGG
                     LVRSSWPEVSEIMAAAHVYTIKRYGPDRVIGFSPIPAMSMVSYSGGSRFMSLIGGTIP
                     SFYDWYADLPPSSPQVWGDQTDVPESGDWWNASYLMMWGSNVPITRAPDAHFMTEARY
                     KGQKTVVVSPDYSDHTKFADHWLPVQPGTDGALAMAMGHVILKEFYVERQVPYFVDYA
                     KKYTDLSMLVTLRERDGADGTPEGAPYVTDRFLHAEDLAETSENAEWKTVFVDATTGE
                     PVVPNGSMGFKYGEEGAGRWNLELGESDPALTFLGRHDALVSLDLPRFDLGDTDEGGT
                     VMRRGVPAKRVGGHLVTTVFDLMLAQYGVGREGLPGEWPGGYDDPEPYTPSWQEEFTS
                     VDSERVTRIAREFARNAERSEGRSMIIMGAGTNHWYHSDEIYRSMLALVLLCGCQGKN
                     GGGWAHYVGQEKVRPITGWTTIAFAFDWARPTRHMAGTALWYLASSQWRYDTFKADEF
                     ASPLGKGKLAGKHMADCLAMSARLGWLPSFPGFDRNPLEITEEAEREGIDVKDYVVRE
                     LKAGRLRFAAEDPDAPENFPRVLTLWRSNLLGSSAKGHEFFLKHLLGVPDATIRADET
                     PAAMRPEEVAWREAPEGKLDLLTTIDFRKNGSSLYSDIVLPTATWYEKHDISSTDMHP
                     FVHPFNPAITPPWEAKSDWDIFNYVAKAFSKLAKKHLGVRKDVIAVPLMHDTPDDLAQ
                     PSGKVLDWKKGECEPIPGKTMPRLVVVERDYGAVAEKMQALGPAVENVGIAAKGNLWK
                     PHQEVEYLKGRNGTVRRGVGAGRPALERADQVCEAILALSGTTNGRLSVEGFKQMEKR
                     TGVKLTDLSEERGEELITFADITTQPRKVIASPEWSGTESRTRRYSPFTINVDRSVPW
                     RTLTGRQQFYVDHEWMLEYGEGLPAYRPPMRMARHTGDLRSGEPGKAEVVLRYLTPHS
                     KWSIHSEFQDNLTMLTLFRGGPTMWISDRDAEKMGVKDNDWIEAFNRNGVVSARAVVS
                     HRIPEGTTIMYHSQDRHLNVPISEVSGTRGGTENSLTKIFVKPTHMIGGYAQLSYGFN
                     YYGPTGSQRDELTVMRKRETEVVY"
     gene            complement(12782..13999)
                     /locus_tag="AC16CM_08590"
     CDS             complement(12782..13999)
                     /locus_tag="AC16CM_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3139929.1"
                     /translation="MSAGTSADGPRPGAYRALALATLAFALCFSVWGLVAPLAPQFQE
                     LYRLSSFQISVVIATPVILGSLFRIPLGLITDRLGGRVVFPVLMLVLIVPVIFIGLLG
                     GSFGGLLFWGFFLGLAGASFAVGVPFVNGWFPPRMQGLALGVYGMGNIGTALAAYSAP
                     AIAAFYGWRWAFWVFVVPLVAMAVVFYVLGRDAPRSGPRSSVTAGISLFRQEVMPWVL
                     SLFYFLTFGGFVALGIYLPKLLVDLFGFTQTGAGLRAAGFVVLATLSRPVGGWLADRA
                     GGARTLMLVFAGLPVTALVLSFEPGIFLFTVGALASAVLFGLGNGAVFKLVAEYYPGK
                     TGAVTGVVGAAGGLGGFFPPLVLGIVRDATGAYTLGFVFLALFAVGCLVVNAMFVRRR
                     PSGSGATTEGRVR"
     gene            complement(14198..14674)
                     /locus_tag="AC16CM_08595"
     CDS             complement(14198..14674)
                     /locus_tag="AC16CM_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3139930.1"
                     /translation="MALILFVHTVHVLLAGAWLGGVVFTTFVVSPALKSMKWDEAQRI
                     GVRAVIGRRYARVGSANLVLLAVFAAVDGVFGGFGTLLYAEYALIVALFGLVAVHGAF
                     FGRRLAGLAKAELEAKSAEEASAFALRRRDLQKLSLKASWVNLAVSVVVLAFAANV"
     gene            complement(14758..15216)
                     /locus_tag="AC16CM_08600"
     CDS             complement(14758..15216)
                     /locus_tag="AC16CM_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3139931.1"
                     /translation="MGDGKRKTKPMKLELSSEGRYALRALVYLAREMDRGELVTADRI
                     SAEALVPRRLLARVMAKLARGGLVESSEGRGGGSRLARPPDEVTLRDAVEAVEGPFEI
                     THCIMQQRPCGEGRPCALHGAWTEGQNTILERLGTRTLAEFSSQAPPFDR"
     gene            15329..15712
                     /locus_tag="AC16CM_08605"
     CDS             15329..15712
                     /locus_tag="AC16CM_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139932.1"
                     /translation="MSSRPHPESERPMHGPLQAFDLGTEVARLREEEAFREGLRNSIT
                     LRKGEGMSVVLLVMRAGDRLEEHAAPGPITVAVREGRIRFATPDGEVEAGPETLLACD
                     AGVRHAVGAVEDAVCVLTIARGGGR"
     gene            15712..16569
                     /locus_tag="AC16CM_08610"
     CDS             15712..16569
                     /locus_tag="AC16CM_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3139933.1"
                     /translation="MYVDVSAGQAVDLAGIASADHGLGAIWVHEGGEDLNVNLVRFEA
                     GEGVGVHRNDEMDVVFVGVAGSGEVVVDGEKFPLKPGLLVYVAKGRLRATRSDLGELA
                     YLTVHRRREPLRLAARVSWDKASVGSGSSEGGYGMKRHPSLRKLSDDHHGGLVRARRL
                     RRAAAGEGESPREAARAFLRFWEEDTSAHFREEEEVLLAVYARHGGDLNADPVREMAA
                     DHAHIRGLVMTLIEEDESGEVSPDTLREMGERLEAHIRLEERSVFPLLESTLTEEGLE
                     ELGARLAPG"
     gene            17435..17800
                     /locus_tag="AC16CM_08615"
     CDS             17435..17800
                     /locus_tag="AC16CM_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3139934.1"
                     /translation="MPTSKKRLRFAERWEQTWREHPETRDLIFPSETGGPMNRNNLLN
                     RHFKPLARKAALPKEARLYTLRHTFATLWMESNEPVKVLQEILGHSRIDQTMNTYAHV
                     MPHIQADAFGRFSKRFSQR"
BASE COUNT         3375 a         5949 c         5905 g         3245 t
ORIGIN      
        1 gtagagcatc caggatagcg tggccaagct cgtgacagac gacatcaggg ctttctccag
       61 agtacacggg tatgccattc accgtattct gaaagaaccg taagccatcg aagcgccgat
      121 actgggcgtt gagcaactgt ccgacaacaa ggttcactgg aaggggagag ctggcagcag
      181 accagcgggt gcccgtaggt agcagcggca tccagaagtt gatgcctcgc gtaagagcct
      241 ctgcggccac ccaataccgg aagtccgggg tgcctggagg ataccgagcg ggcaaaggct
      301 gcgggtcgcg gatagcaaac cgcagcaacg gtgcattaat ggtagtcacg tcgacaacca
      361 ccggatctgg gcttaccggg ttcgtggttg gagtagcttc gctgaagggg tcgtcttccc
      421 aagtgttgat ctgcggtacg gatgaggccg caggcaaaac gccgccggcc gggaactggg
      481 cgatgtcctt agcggacgta ccatcaggca agccctgcac agtttgcatc atttgcgtcg
      541 cggccgccgt aagggtctgg ctagcctgtg tcaatgcccg taccgcagtg acaacgacgc
      601 tctctgtaac accgctcgtg ggtgcctcgc tgttcgagcg ggcgtccacc gtgtcactat
      661 tgtcgatcat gtttggctcc cttccgagtc aatgcggttg tcgcactact aacgcgagcc
      721 gccacttctt cgtcgactag cacacctacc ccctcttgcc tcctttccca agaatgaagc
      781 tctatctccg ttgctcctat atgcacagat cagcgagggg tcaggttcac ctcaaagcac
      841 catttaagcg aacagcgatg aatagagaat gaattttcgg cgggaacgga aacccatggt
      901 ccgattctgg gatactgctg ccgtcacgaa tcgtatgcgg ctatgtacat atcttctgaa
      961 agtatatggc cggtagtttt gcgactctcg caacgccctt tttcggaggt gaggtggcgt
     1021 tgagctgagg ccaagtggag atgcggacct ccagaatgct tatcctgaag aaccgtgtgt
     1081 agtgtgacgg atcgcatatg cgttcgagcg gacctacgac ggtgtggcag cgatgctcga
     1141 cgaactcgcg atcaggtacg atgtcccctg cctgtgacgg tcacagacgg ccttgaagtt
     1201 ctcgaccgcc gcgtggcgcg acttatggaa gatgcgcctg acctccgctt ctgggtcagt
     1261 ctgcgggtaa ggtccccggc gtccgctcgc tatccccgaa gacgaagtgc tctttttcgg
     1321 gcaacttacc tttcgatcgg cgcttcggcc agcctactag cgtggatgtt ggcggaataa
     1381 ggtacgcggc gatgggtatc tatcgatggg tatgtaaagt cggccgccac cgtgcggaag
     1441 agtagggatg atgcgcttat ttacctctac tccttgagag agggcggcac tcttcggcct
     1501 cgcctaagtg tgaaggagtt gatcgacgtg cccgcttgca ggggctgctg caccttccga
     1561 gaacacctgt gcggcgaagt cgttgaggaa ctttccctcg gggtcttgtc gttcacgcgc
     1621 acgctggaac acgacgaagc gctccccgtg catggtctcc attcccgcgg aggtcacctt
     1681 cgtcgccttc cctaggtgag ggtgtcctcc gtacttgagg aagatcttct ctgcctcgtc
     1741 gaacatctcc gccgggtcca gcgacaggct aggcgcgagc tcgatgtaac acgtccgccg
     1801 accggcgccg gggccgagga gcccgttcga tttgtcgggc gtgaagcgca cttcgatgat
     1861 ggtgacgtgc cccttctctc ggagcatggc ctccaattcc ttcatgcact cttggtgagc
     1921 ctcgtagggg atgccgaact cgatctcgtc gtggcggtag tagagcttgc gggcgaatcc
     1981 ctccgccgag gaatagacgt cgctcctgcc gtccatcgtc agcttgaaga ataggagccc
     2041 gagcgccgcg gtaggccccg tcagcttgag ggcccgcgcg aggccgagca agtagcccga
     2101 gaacagcatg tcgatgagct cgttgcgcat cttccgcagc ttgatcgtcg cgggtggctc
     2161 caaaacggtc cggttccaca ggttcatgcg aacgcgctcg acgccgccgc cgccgatgta
     2221 ataaaagctc gtgtggtcgt tctcctcgag gatggcgtcg atgttcctct cgacccagtc
     2281 gcgccgcaca acacggatcc ctttgcccag gttgaagtcc ggtacgcatt ccagctcgac
     2341 ctcggtgacc accccgcagc tgccgagccc gccgaacgcg gcccgaaaga cgtcggagtc
     2401 ccgccggaac gtctccgctt ttccggcggc gttcacgatc cgcagcgaca gcacctgctc
     2461 ggacaggaag ccgtgatccc tcccagtacc gtggaggtcc gtcgctatca ggccggcgag
     2521 gctctgggcg ttggtggagc ccagcacggg cagggccagg ccacgctccc cgagttcttg
     2581 catcagatca cgcagccgga cccccgcttg cacccggacg accctgtttt ccgtgtctac
     2641 ggccaagatc ttgttgtact tgtcgaggga cagcatcgtc tctaccgaga gcggagaggg
     2701 gttgaacgtg tggccaccgc cgatgacgcg cacgctcttc gataatccga ccatctcgca
     2761 gattgccgct tcgctctccg gggtctcaaa cctctgtggc cgggcagaga atgtgttgtt
     2821 ccagttggtc cacctgccgg tcgagcggtg gtagcgtccc tcgtgaagcg tcttcgtacc
     2881 gcctgatagc acgtacgcgg cactgttcaa ggtattgttc gcccacgtgt gcacgacacg
     2941 ctttatccgc tcaaaagtct tcatggactc cctttctctc gatgagctcg gtatcgtcct
     3001 cgtaggacga tccggttccg tttcaggtcg tgaacaacgc gtacgacgtg cctacgatcg
     3061 catctgcgag ggggcccttg agcacctgcc aaggagaaag tggcgccgtg gtagccaatg
     3121 ctcgagtaag ctcgccgaag cgttcctagt ctgtccccta cacctcctcg tacggacgca
     3181 gcatcgtcgc cccgccgttc ggcagcccgg ctacctcgca gccaggtggc tcttgggctt
     3241 cgcgctcccg cctcacgaag ggtatgcctt tctcgctaga acacacggat gcgcaggcga
     3301 gcaggcacag tccaagtatt cgatacaaat gtagcataag ctgcttgcta acctcacgca
     3361 acacacctcg gaggatgtat cctgcacgta gacggtgctg tcccgtgctg ctttgcgcca
     3421 cgctccgtcc tcggtcatgc ccgccttatt accagggcgc gatgaattcc gaatgaattt
     3481 ttggcgcagc tgctcagaag cgtcagcccg aatgtcaccc tgttgtcaag gtctgcaacc
     3541 gccgcacccg gttgcgtcca taagattctg caaaaggggc cagaagggaa ggcaccctcc
     3601 tacaagattg ttggtgctca agtcaactct tgaggaggga aagatggccc gccacaatcg
     3661 taaggtggac gaggagaggg tgcaagaagt cctactggat gaccccggct tcttgcgtga
     3721 gatcatctag cagatccttc aggagatgtt ggaggcctag agaccgaaca cgtaggcgcg
     3781 gctcactacg agcgtagagc cgcccgcacc ggccaccgca atgactacaa gccgagaacg
     3841 ttgcgcgcca gggtcggcac cctcaacctg ctcgtccctc aggataagga aggaaccttc
     3901 tccacctggg taaggagaag gtagccgagt acatcgagga gtacgtggag gaatgcctca
     3961 cctgcctgag cttctcgtag tctcatcgta ggtgcatccg caccaccacc ggcccgataa
     4021 cggacgtcga tagttggggg acggagctta cagacttcgt cccttcttcc ttcccttatt
     4081 cacccaacgt tggggaaggc ttagtctcgg aactgcagac gcaggatcct gcatggacca
     4141 acgccgagta cccgctaggg cgagccatct gcctccggaa cgccacgagc ctaacgcaat
     4201 atgcgtggtg ccggataaag atgcgaaacc gtgcacaaga tccggcaaga caccgcgcaa
     4261 gagcgaaccc gaccgcgagt agaattccct tgaaccatgg ccatctacct acgtgggatt
     4321 tgtggtcagg tcgggcctgt ctctatcaag acctctgcgc cgcgaagcgc ttcgcaagct
     4381 ctcatcgaga ccccggaatc ctacgccccg ctcctcgagc tcccccacta agccgatcaa
     4441 ctcccggagg gatctgccca gcctgtcgag tcgcgccacc accagcacgt cgccgttgcg
     4501 gcagaattcg aatgcttccc gcaacgtttg cctatcagcc tccctactgg agattgcacg
     4561 cctggtgcgc ggagccacgc taagaaacta cgtaccgcaa cacttctcgt tgcccctctt
     4621 gcccgtagtc tggattttgg cgacggaatc ggcgagcgcg aggttcctcg cgcatgtctc
     4681 aggtgtagac ccgagcctct tcgcgacttg gccggtttct gtggggtcga agcgtgcggc
     4741 ttcgggaccg gtaaacgcag aagtcggtgt ctggagctag ggatctatca aggcacgaac
     4801 tatcgtcctc gcgcactctt cggggtcccc agcctgccga gcgctcaact cgacctcggc
     4861 ggcacggata ccgtcggtgc aaagctctcg aggaagctct tcgaggatcg tgccaaccgc
     4921 ggcccgccat gcgcgcagga gtggatcacg ggtgcccccg ctatcttcga cgccggccat
     4981 gaccacgtgg ccccctccgc gttcgcgcat ggctggcaag gccgcggcca ggcagtagac
     5041 gaagcccctc acgttcacgt ccagggaacg ttcccaggcg tcgaggttga ggcttcccag
     5101 gggcggggcg gcggcagagg ccatgtacag cagtacgtcg agcccgccga aggtttccac
     5161 cgcggccgcc accaggtgcg ccgggtggtg tcgcttcgcg aggtgtacgc cgacgaccag
     5221 cgcctcgccg ccgtgcgccc ggatctcctc ttccaactcc tcgagcctct gccggtcgcg
     5281 tccgccgagg gcaacgttgg cgccctggcg tccgagctcc ctggcggcgg cggccccgtg
     5341 ggggctggag ccgccgagta ccgcaacggt cttgccggcc agcgtccccc tcatcgcccg
     5401 cccgcgaggg gcccggcgcc ccccgagccc tcaaccgggc cggtctccca ctcgcccagc
     5461 ttcccgccga agaagccacc gaggacgttc agaacgagca aggcgaggcc ggcgatcaag
     5521 gcgccgcccg cgagcccgag cgtcagggcg gggagcgcga cgcccccttc gaccaggaag
     5581 agcccggcgg tggccaccac gatggccgag gttatcaggg cggcggccac cacgaacagg
     5641 aacccgagca ccgccaccat cacgccttgc aagcccccgt gggctcgggc cttcctgccg
     5701 gctacgtatc ccccggcgaa gtgggccaaa aaaccggaga gcaaagagag cgtcacgagg
     5761 gcggtcttga ggttgtccgg gttcatgacg tcgccgccga agagcaggac gtgggccgcc
     5821 tcgaagagga ggttgagcac gatgcccgcc gcgatcgcca cggcccaacc gacgatcacg
     5881 gccctccagt tcgtcttctc cgacaggccg tggaacataa gactatttct cccgccgaat
     5941 cattcgacgc ggctgctctg ctcggccttg gccggcccct tgccggccct cgctcgcgcg
     6001 agggtctttt gcgggttcga gctacggtac tggacgtagc tgcggcgcag gtagcctagg
     6061 gggaagctga agacgtgcac gaggctggag aagggccaca gggcgaagag gacgaacgcc
     6121 gagagcgtgt ggagctggaa gatcaggggg gcgccgacca tcagggcggc gtcgggacgg
     6181 aacaggaaga ggctgcggaa ccacgcgccc acggaatccc ggtactcgaa ctcaccgtgc
     6241 agttcgtact ggctagcgta gacgcggtag ccgacggtcg cggcgagacc caaaaagatc
     6301 accaccagga gcaacccgtc cgtcaggaac ctgatcgatt cggtgttgtt tcttatgcgc
     6361 ggtatgaacc agcggcggat gttcaggagc aggatgccca ccatcgcgac gaccccgacc
     6421 ggcgccccga cccacaagga tagcaggtgg tagttgtggt ccgagacccc gatggcgcgg
     6481 tagacgccta agggcacgag caggcccatg acgtggccgc cgaagacgaa aaagaagccc
     6541 cagtggaaga gcaggacgcc ccacatcagg aggcgccgct ccagaagttg actggacttc
     6601 ggcgtccagc cgtactggtc gtagcggtag cggtagacgt gcccgagcac gaacgtcacc
     6661 agggagaggt acgggaagat cacccacaag aactggtgcc acgcgttcaa cgcttcctcc
     6721 cgtggaaagc gacgtggcct gtgggagcgg cgccacccaa agaaccctcg ttgccgaaag
     6781 gattccccgg cggcacgtcg gccgcgtagg gctccagccc cacgctttcc tgcggcggcc
     6841 cctgcacgat gagcttctgc atctccgccg cggtctcctc gtccaggtcc ggcaggaaga
     6901 gggtgagggc ctcgacgagg tgcgagtagg gactattgtc gtggcgcagc gagcgccgga
     6961 tgacctctat ggcagcacgg ttctcggcaa gcatgggaag tccctcctcc agaacctccg
     7021 aggcgaactc gaggacgagc ggcaggtagt cggggagctc tgtggtgtcg agcgggtagc
     7081 cggcccgcgc gtaggccgcc ttcatgtcgg cgagaacctg cccgcgggcg cgctggtccc
     7141 cgtagcggta gtagctcagg tacaggcagc cgcggcgctt gaaatcgaag gccccggtgt
     7201 atgatgcctg caggcgggag gcgggctcgg actcccagtg ggccaggaac gccaggatgt
     7261 tgtccttggc cggggagtcg gggaggccgc gggcggcctc ggagatctcg gccctgtgct
     7321 ccatgagccc gtcgtctggg tagcgcagca ggatggagag aagcttgaag atcacgcgtc
     7381 tgcccccttc ggcccgcgcc ccttgaggtg gaacgccgcg aagtccttct tgctagtgaa
     7441 gagctgcacg ttgggcgcct gcgccttgcc gccgagctcg cgggccttct tctcgagctc
     7501 gtctttcatg gcgtagaagg cgccgttgcg gcccccgcca agggtggcgg cctccgccga
     7561 agaggagcag ctccccgggc cgccggcgaa gtcgaggccg cacgcgtgcg cgtcgtttcg
     7621 gtgctcgact tcggccgggg tgcgcccccc ggcgaagtcg agcccggccg ttccgcgctg
     7681 ctcgtagagc tcggcggcga gctccttgtg ggcgagcggg atgacgtagc ggtcctcgta
     7741 cttggcgatg gcgagcaggc ggtacatgtc ctcgatctcg cccggctcca tcccgacggc
     7801 tgaggcgatc tcggcgtccc cgatacccga gaggtccttc tcgcgcatgt agccgcgcat
     7861 cgcggcgagc ttcttgagga cgcgccggac gaccccctcg tcgccggccg agaggaggtt
     7921 ggcgagatac tggatcggta tcctcagctc gtctatggcc gggaagacgt cgtcggggtc
     7981 ggcctccgcg ccgttgccct cgacgaagcc catcaccggc gagagcggag gcacgtacca
     8041 gaccatcggc atcgtgcggt actcggggtg cagggggagt gctatgcccc attctatggc
     8101 gaggcggtag atcggggatc tgcgggccgc ggagatccaa tcgtccggga tgccgtcgaa
     8161 gcgcgcctgc tcggcgacct ccggatcgtt cgggttcagg aacacggagc gctgggcctc
     8221 gagcagatcc ttctcgtccg ggacggatgc cgcctcctca accccgtccg cgtcgtagag
     8281 gacgagcccg atatagcgca ggcggcccac gcaggtctcc gagcagatgg tcggctgccc
     8341 ggtctcgatg cgcgggtagc acagggtgca tttctcggcc ttgccggaat cccagttgaa
     8401 gtagaccttc ttgtacgggc agccggagac gcagtaacgc catccgcggc actgctcctg
     8461 gtcgacgagc acgatgccgt cctcctcgcg cttgtacatc gcgcccgagg gacacgacgc
     8521 cacgcacgag gggttcaggc agtgctcgca gatgcgcggc aggtacatca tgaagacctt
     8581 ctcgtactcg aagcgcaccg actcctcgag ctcgccccgg atgttcgggt cgaggtgtcc
     8641 ggtctcgggt gcacctgcga ggtcgtcgtc ccagttggga ccccactgga ggtcgacggg
     8701 ctcgccggag agctgcgata taggacgggc caccggctgg tgctccgaga gcttggcgtt
     8761 ggtgagggtc tcgtagtcgt aggtccaggg ctcgtagaag tcgtctatgg atgggaggtc
     8821 cggattgaag aagatgttgg ccagcttgcg gagcttgcct ccggccttga gctcgagctt
     8881 gccgttcttg cccagagccc agccgccctt ccagcgctcc tggtcctcat agagcttcgg
     8941 gtagccgacg ccgggcttgg tctcgacgtt gttccaccac tggtactcgg tgccctcgcg
     9001 gctcgtccac acgttcttgc aggtcaccga gcaggtgtgg cacccgatac acttgtcgag
     9061 gttcatcacc atcgcgacct gagcctggac gcgcatcagt agaccacctc cgtttcgcgc
     9121 ttgcgcatga ccgtcagctc gtcgcgctgt gagccggtcg ggccgtaata gttgaagccg
     9181 taggaaagct gggcgtagcc cccgatcatg tgcgtcggct tcacgaagat tttggtgagg
     9241 gagttctccg tcccgcccct cgtgcccgag acctcgctaa tgggaacgtt caggtgcctg
     9301 tcctgggagt ggtacatgat cgtggtcccc tccgggatgc ggtgggagac gacggccctc
     9361 gctgaaacca ccccgttcct gttaaacgcc tcgatccagt cattgtcctt tacgcccatc
     9421 ttctcggcat cccgatcgct gatccacatc gtcggcccgc ctctgaagag cgtcaacatc
     9481 gtcaggttgt cctggaactc ggagtggatg gaccacttgg agtgcggcgt gaggtacctt
     9541 aggacgacct ccgccttgcc gggctcgccg ctcctcaggt ccccggtatg cctggccatc
     9601 cgcatcggcg ggcggtaggc tggcaggccc tcgccgtact cgagcatcca ctcgtggtcc
     9661 acgtagaact gctggcgccc cgtgagcgtg cgccagggga cggagcggtc cacgttgatc
     9721 gtgaacggcg agtagcggcg cgtgcggctc tcggtgcccg accactcggg agaggcgatg
     9781 accttgcgcg gctgggtcgt tatgtctgcg aacgtgatga gctcctcgcc acgctcctcg
     9841 gagagatcgg tgagctttac gccggtgcgc ttctccatct gcttgaagcc ctcgacggag
     9901 aggcggccgt tggtcgtacc ggagagggcc agtatagcct cgcacacctg gtccgcccgc
     9961 tccaaagccg gcctccccgc gccaacgccc cggcgcacgg tgccgttgcg tcccttgagg
    10021 tactcgacct cctggtgcgg cttccagagg ttgcccttgg ccgcgatgcc cacgttctcg
    10081 accgctggac ctagggcctg catcttctcg gcgacggctc cgtagtcgcg ctcgactacg
    10141 accagcctcg gcatcgtctt gccggggatg ggctcgcact cgcccttctt ccaatcgagc
    10201 acttttccgg agggctgggc gagatcgtcg ggggtgtcgt gcatgagggg aaccgcgatc
    10261 acgtccttcc tcaccccgag gtgctttttg gcgagtttgg agaacgcttt ggcgacgtag
    10321 ttgaagatgt cccagtcgct ctttgcctcc cacgggggcg ttatcgcggg gttgaagggg
    10381 tggacgaacg ggtgcatgtc cgtagatgag atgtcgtgct tctcgtacca cgtcgccgtc
    10441 ggcagcacga tgtccgagta gagcgacgac ccgttcttgc ggaagtctat ggtggtgagc
    10501 aagtcgagct tgccctccgg cgcctcgcgc cacgccacct cctcggggcg catcgctgcc
    10561 ggggtctcat cggcccggat cgtcgcgtcc ggcacaccga ggagatgttt caagaagaac
    10621 tcgtgaccct tggccgaaga gcctaatagg ttcgatcgcc agagggtgag cacgcgcggg
    10681 aagttctccg gcgcgtccgg gtcctcggcg gcgaagcgca ggcggcccgc cttcagctcg
    10741 cgcaccacgt aatccttgac gtcgatgcct tcccgttcgg cctcctccgt aatctccagc
    10801 ggattgcggt cgaagccggg gaaggacggc agccagccga ggcgggcgga catcgcgagg
    10861 cagtccgcca tgtgtttgcc ggcaagtttg cccttgccca gaggcgaggc gaactcgtcg
    10921 gccttgaagg tgtcgtagcg ccactggctg gaggccaaat accagagcgc cgtgccggcc
    10981 atgtggcggg tgggcctcgc ccagtcgaag gcgaaagcta tcgtggtcca gccggtaatg
    11041 gggcgcacct tctcctgacc gacgtagtgg gcccagccgc cgccgttctt gccctggcac
    11101 ccacacagaa ggacgagggc tagcatggag cggtagatct cgtcggagtg gtaccagtgg
    11161 ttggtgccgg cgcccatgat gatcatggac cgaccttcgc tcctctccgc gttgcgcgcg
    11221 aactcgcgcg ctatccgcgt gacgcgctcg gagtcgacgc tcgtaaactc ctcctgccaa
    11281 ctcggtgtgt acggctcggg gtcgtcgtat ccaccgggcc actcgcccgg cagcccctcc
    11341 cgccccacgc cgtactgcgc gagcatcaaa tcgaagacgg tcgtgacgag gtgcccgccg
    11401 accctcttcg caggaacgcc ccttcgcatc acggtccctc cctcgtcggt gtccccgagg
    11461 tcgaagcgcg ggaggtccaa ggatacgagc gcgtcgtgcc tacccagaaa ggtcaaagcc
    11521 gggtcggact ccccaagctc gaggttccac ctgccagccc cctcctcgcc gtacttgaag
    11581 cccatcgagc cgttcggcac gaccggctcc ccggtcgtcg cgtctacaaa cacggtcttc
    11641 cactcggcgt tctcggaggt ttcggcgagg tcctcggcgt gcaagaagcg gtcggtgacg
    11701 taaggggcgc cttcgggagt gccgtcggcc ccgtcgcgtt cgcgcagggt cacgagcatc
    11761 gagaggtcgg tgtacttttt cgcgtagtcg acgaagtagg gaacctgccg ctcgacgtag
    11821 aactccttga ggatgacgtg gcccatagcc atggccagcg ccccgtcggt gcccggctgg
    11881 acgggaagcc agtggtccgc gaacttggtg tggtcggagt agtcggggct cactacgacg
    11941 gttttctggc ccttgtagcg ggcctcggtc atgaagtggg cgtcgggggc gcgggtgatg
    12001 gggacgttcg agccccacat catcaggtag gaggcgttcc accaatcgcc cgactccggg
    12061 acgtcggtct gatctcccca gacctgggga gaggagggcg gaaggtcggc gtaccagtcg
    12121 tagaagctgg gtatcgtgcc cccgataagc gacatgaacc gtgatcctcc ggagtaggag
    12181 accatgctca tcgcggggat cggggagaac ccgatgacgc ggtcgggccc gtatctcttg
    12241 atcgtgtaca cgtgggccgc ggccatgatc tccgagacct cgggccacga ggaccgcacg
    12301 agcccgccct tgccccgtgc cgccttgtac ctcctggcct tctcgggatc ctcgactatc
    12361 gacgcccagg cgtccacggg gtcgccgatg cgcgcgagcg cctcgcggta catctcaacc
    12421 aggacgccgc ggacgtaggg gtactttatg cggatcgggg agtaggtgta ccaggagaag
    12481 gaggccccgc gcgggcaccc gcgcggctcg tactcgggcg aatcggggcc gttggagggg
    12541 tagtcggtct gctgggactc ccaggtgatg atgccgtctt tgacgtagac cttccacgag
    12601 caggatccgg tgcagttgac gccgtgggtg gagcgcacga tcttgtcatg ctgccacctc
    12661 tggcggtagg cgtactccca ctccctgtcg cgcgcgctga gctcggcgtg cccgtccgag
    12721 cgcttctcgc cccggcggaa gtactgcaag ttcctgagaa aagcattcgt ggacttcgcc
    12781 actaccgcac cctcccttcc gtggtcgcgc cactacccga cgggcgcctt ctgacgaaca
    12841 tggcgttgac gacgaggcac ccgacggcga agagcgccag aaatacgaac ccgagggtgt
    12901 acgccccggt cgcgtcccgc acgatgccca gcaccagggg cgggaagaag ccgccgagcc
    12961 caccggccgc cccgacgacg cccgttacgg cgccggtctt gcccgggtag tactcggcaa
    13021 ccagcttgaa gacggccccg ttaccgagcc cgaagagcac cgcgctcgcc agcgccccca
    13081 ccgtgaagag gaagatcccg ggctcgaagc tgaggacgag cgccgtgacg ggtagccccg
    13141 cgaagacgag catgagcgtc cgggcgccac ctgcgcggtc cgcgagccag cctcctaccg
    13201 gacgcgagag cgtggccagc accacgaagc cggccgccct gaggccggcc ccggtctgcg
    13261 tgaagccgaa gaggtcgacc agcaacttgg gcaggtagat gccgagcgcc acgaacccgc
    13321 cgaaggtgag gaagtagaag aggctcaaga cccagggcat gacctcctgg cggaacaggg
    13381 atatcccggc cgtcaccgaa gatcgcggcc ccgacctggg ggcgtcccgc ccaaggacgt
    13441 agaagaccac cgccatcgcc actagcggca cgacgaagac ccagaacgcc caccgccagc
    13501 cgtagaaggc ggctatggcc ggggcgctgt aggcggcgag cgccgtccct atgttgccca
    13561 tcccgtagac gccgagggct aggccctgca tacgtggcgg gaaccacccg ttgacgaacg
    13621 ggacgccgac ggcgaacgag gccccggcga gtcccaggaa gaacccccag aagagcaggc
    13681 ccccgaaaga gccgcccaag agcccgatga agatcacagg cacgatcagg accagcatca
    13741 gaacaggaaa cacaacccgg ccgcccagac ggtccgtgat caaccccagg gggatgcgaa
    13801 acagcgaacc caggatcacc ggcgtcgcta tgaccacgct gatctggaag ctggatagcc
    13861 ggtacagctc ctgaaactgc ggcgccaacg gagccacgag cccccaaacg gagaagcaca
    13921 acgcaaacgc caacgtcgcc agcgccagcg cccggtacgc ccccggccgc ggcccgtcgg
    13981 cgctcgtgcc cgcgctcatc ccccaacccc ctctcccgaa acggcgaaaa ctgctgcgcc
    14041 cactttccat accggacctt cccccacagc ccaactcgac gaggttcgac caacctcctg
    14101 atccggaaat accaactcat gcgaagctac gcgcaagggg aactgcatcc cccacccgat
    14161 ccctcggttg actccccgga gcaccatctc accgtcccta gacgttcgcg gcaaacgcta
    14221 acaccaccac gctcaccgcg aggttcaccc acgacgcctt gagcgagagc ttctgcaagt
    14281 cgcgccgccg cagcgcgaaa gccgaggcct cctcggcgct cttagcctcg agttcggcct
    14341 tcgccaatcc cgcgagacgc cggccgaaga acgctccgtg gacggccacc agcccgaaca
    14401 gcgctacgat caaagcgtac tcggcgtaaa gtagcgtccc gaagccgccg aagaccccgt
    14461 cgacggcggc gaagactgcc aacaggacca ggttggcgct accgacgcgc gcgtagcggc
    14521 gcccgatcac ggccctcacc ccgatccttt gggcctcgtc ccacttcatc gatttgagcg
    14581 cgggagatac cacgaacgtc gtgaacacga cgcccccaag ccaggcgccc gccagaagga
    14641 cgtggaccgt atggacaaag agaatcagcg ccacctccca tactgttacc aatattgtga
    14701 tagtaaatgt ccgagtatcg cggcgtcaag ggttgggaag aattcgggcg gcggaagcta
    14761 gcgatcgaag ggcggggctt gggaggagaa ttcggcgagg gttcgggtgc cgaggcgttc
    14821 gaggatggtg ttctggccct cggtccaggc gccgtgcagg gcgcacgggc ggccctctcc
    14881 gcagggtcgc tgttgcatga tgcagtgcgt gatctcgaac ggtccctcca cggcctccac
    14941 ggcgtcgcgc aaggttacct catcgggcgg ccgggcgagg cgggagcctc ccccccggcc
    15001 ctcgctgctc tccacgagtc cgccccgggc gagcttcgcc ataacccggg cgagcagccg
    15061 gcgtggcacc aaggcctcgg ccgagatcct gtccgccgtc acaagctcgc cgcggtccat
    15121 ctcccgcgct agatagacca gtgcccgcag cgcgtaacgg ccctcgctcg agagctcgag
    15181 cttcatcggc ttggtctttc gcttgccatc acccacaaga tacaccgcca cgccgcccgc
    15241 gtgaaagcgt tcgcccgccc ttccttcata ctgacaccaa ttatgtcata gttaaagaac
    15301 tggagacgtc cgtcgagagg agaaagagat gtcgagcaga ccgcatcccg aatcggagcg
    15361 gccgatgcac ggaccgttgc aggccttcga cctcggtacg gaggttgcca ggttgcgtga
    15421 ggaggaagcg ttcagggaag gacttcggaa ctcgatcacg ctgcgcaaag gcgagggcat
    15481 gagcgtggtg ttgctcgtga tgagggccgg agacaggctc gaggagcacg ccgcgccggg
    15541 ccccatcacc gtcgccgtca gggagggccg catccggttc gccaccccgg acggggaggt
    15601 cgaggccggg ccggagaccc tgctcgcctg cgacgccggg gtgcgccacg ccgtaggagc
    15661 cgtagaagac gccgtctgcg tgctaaccat cgcgcgtggc ggggggcgct gatgtacgtc
    15721 gacgtctcgg cgggccaagc ggtggacctc gccgggatcg cgtcggccga ccacggcctg
    15781 ggagcgatat gggtccacga aggcggcgag gacctgaacg tcaacctggt gcgtttcgag
    15841 gcgggcgagg gcgtcggggt ccaccgcaac gacgagatgg acgtcgtctt cgtgggcgtc
    15901 gcgggttctg gtgaggttgt ggtcgacggg gagaagttcc ccctgaagcc ggggctgctc
    15961 gtctacgtcg cgaaaggtcg cctgagggca acccgcagcg atctcggcga gttagcctac
    16021 ctcaccgttc accggcgaag ggaaccacta cggctggccg cgcgcgtctc ctgggacaag
    16081 gcatcggtgg ggtcaggatc gtcagaagga ggctacggca tgaagcggca cccatccctg
    16141 aggaaactct cggacgatca ccatggcggg ctggtgcggg ccaggcgcct ccgtcgggcc
    16201 gccgcaggag aaggggagtc gccgcgtgag gcggcgcggg cgttcctgcg tttctgggag
    16261 gaggacacga gcgcccactt ccgagaagag gaggaggtgc tgctggccgt gtacgcgagg
    16321 cacggcggtg acttaaatgc cgatccggtc cgagagatgg ccgcggacca cgcccacatc
    16381 cggggcctcg tgatgaccct gatcgaagag gatgaatcgg gcgaggtcag tccggacacg
    16441 ctgcgcgaga tgggggagcg tctcgaggcc cacatccgcc tggaggaacg gagcgtcttt
    16501 ccgctcttgg agtccaccct caccgaggag ggtttggaag aactaggagc acgcctcgca
    16561 cccggttaag cggtccctct cgagtcgctt ccgacgatca acctgcaagg gtgccgaagc
    16621 cgcgcttggc gtgatcgccc cgcggctcac accgcctatc gtcgggaggt ttcgtcgttc
    16681 agaggcgatc ctcttgcgcc gccgactcgt acttaccgcg ggagcccttc tccggtacgg
    16741 ccgacagtaa cgccattttg atcggtcgtc gctttgcaaa cctgtcggcc aaccctgtaa
    16801 gggacgcttg ttgtacatgc aggatcgtgc acgcgaattt ctgagaaggg gtgaatccgt
    16861 ggcccccacc cctgcatggt acgaaccgcc cggggcagcc taacctttct gcggaggggg
    16921 actttggtgg cgtcgctccg tggttcgggc cgcagactgc ccttgagggg cggcctccgg
    16981 ccctcatgga tgctggtgcg gaagggcggt aggccgcttc gctgttcagg gtgctagttt
    17041 aggaccgaac tgaagcccag aggacggcgg ggcgtgcgag agggggggga acctgacagt
    17101 aaagccaaat tggttatctg acgacctcta tgctgacatg gaagcaaagg tgtcggggag
    17161 cgcaccgccc gctacctcca ccaggtcgcg agtgctgcgc tcgagagggc cgcaaagaag
    17221 ctcctgatcc cgttcagcgt cgtccgctac gccgaccctc ccagagagcc cagcgtcccc
    17281 ggtcaagcga cgatccgccg ccgcgtctct atagatggct ccgggagatg ggagatcctc
    17341 gacgggacga agacctcgcg caaaaacaag cgcgccaagg cacgcacgct ctacctgatg
    17401 ccaggggcag tggaggcgct gaaaagcgaa cgcaatacct acctcgaaaa aacgtctgcg
    17461 cttcgccgag cggtgggagc aaacctggcg cgaacacccc gaaaccagag acctcatctt
    17521 cccctccgag accggcggcc ccatgaaccg caacaacctc ctaaaccgcc acttcaaacc
    17581 gctcgcccgg aaggccgcct tgcccaaaga ggcgaggctc tacaccctca gacacacctt
    17641 cgccacgctc tggatggaat ccaacgagcc ggtaaaggta ctccaggaga tcctcggcca
    17701 ctcaagaata gaccagacga tgaacaccta cgcgcacgtc atgccgcaca tccaggccga
    17761 cgccttcgga cgcttctcga agcgcttttc gcaaagataa gtggtcaaat aagtggtcaa
    17821 agccgctcgg aggcgatggg tcagaaacga aaccccgcga tttgcggggt tttctcctgc
    17881 gcgctcggca ggactcgaac ctgcgacctt ctgattccta gaccgaatag agcagttcta
    17941 tgcggtcctt cagagtgcgt taagcgcgat ttctaagcgg ttttcgtgga ttagggtatc
    18001 ggctagttct gccctgctcc cgtcctactc ccgtcgcgct cccgtcaaaa ttggcacccg
    18061 gtatgtcgat gggagattcg catgctagct ctggcataac gtcgtaccac ggttagtcgg
    18121 cggcccgggc ccgtacggtg cggcgagtcg gaagccgaag aatcaagggg aggaaagagg
    18181 ttcaatgata atgcatcccg tcgcgtcagg acgtttgtct caatcaagac ttacgcagtt
    18241 gattgagggt accatctggc gcaggtgtct accggcgcgg agtaatatct caagcagatg
    18301 ctattcaggc cctccatagc cgcacatgcc catgcgcggt gagttcacta ctggaagcag
    18361 catctccggc tcttcgcaat cgtggtcctc ggcgtgaagc ttgcaatagt aggggttctc
    18421 gcgctcgtac atgcagaagg tgcagatcca gtcggcatcc tctccgcaga cctc
//
LOCUS       DBNGVD010000321         2736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1530701, whole genome shotgun sequence.
ACCESSION   DBNGVD010000321 DBNGVD010000000
VERSION     DBNGVD010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1530701"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="AC16CM_08620"
     CDS             complement(<1..289)
                     /locus_tag="AC16CM_08620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3139935.1"
                     /translation="MRALEGKKALVTGAGMGIGQGIAVELARQGAEVAVHYAHSEPDE
                     TVSRIEQLGGRAATIQGDLSNTAECEKTVDAAAEALGGLDALINNAGITKTA"
     gene            complement(286..1452)
                     /locus_tag="AC16CM_08625"
     CDS             complement(286..1452)
                     /locus_tag="AC16CM_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3139936.1"
                     /translation="MKITHLDTFVVGTEWRNLTIIRAHTDEGLTGLGEARMVNHTDAL
                     VGYLAEAAPRHLIGHDPFRIEELVRKMGRDDFSRAGEVMSSGIAMFETACWDIVGKAL
                     GQPVYNLLGGSVRDRVKAYANGWYRVERTPEEFHEAAKKVVERGYRALKLDPFGPGHY
                     ELEYEEKLRAVSLVEAVRDAIGPEVEILLEMHGRFSPATAIEMARMLRPFGLGWVEEP
                     VPPVNLKALKKASDGIEDTVATGERIHQRLEYREVFELQAADVIQPDISHIGGLLETK
                     KLAAWADAYYMTIAPHNVGGQINTAAALHLAAATTNFRIQEYFNDFADPWVRETAPGL
                     PEVVDGYFELPKGPGLGVELDEDVIAAHPKQNVHFNLFSEGWEKREGARTGADG"
     gene            complement(1449..2396)
                     /locus_tag="AC16CM_08630"
     CDS             complement(1449..2396)
                     /locus_tag="AC16CM_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR3139937.1"
                     /translation="MRAHELLSGLGMPGRDLHDPPDSGKRFPDGAQYRVEIPSVEGPR
                     VLEAVIDEARKREVQIHRVSQGSGIMLLTDAEILEMCQVAREAALELSLFVGPRASWE
                     TGATAISGAGKVLGAQHRGQDQLAYALEDVLRGVSLGLRGVLVADAGLLWVLRDLKAK
                     AELPEDLVLKVSVQLAAANAAAVKAMEDLGADTYNVPTDLSMAQLAAIRAVADLPLDV
                     YVEVPDDFGGFVRHYEIPDLVRVASPVFVKFGLRNAPNIYPSGTHLEATAVALGRERV
                     RRARIGQEMLARYYPDAETTPPGAVFPGLPTDAEVKEKA"
     gene            complement(2393..>2736)
                     /locus_tag="AC16CM_08635"
     CDS             complement(2393..>2736)
                     /locus_tag="AC16CM_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3139938.1"
                     /translation="MRRSIEAPEGYVDADVEFHALLAQGARNEVLLTMLEPVVELLRA
                     SRRVSAARPGNAQRALAEHEQILRHVEAGDAAGARREMRAHLAKTSEDIEAAIGEGML
                     DTGEFKEKEGV"
BASE COUNT          372 a          968 c          879 g          517 t
ORIGIN      
        1 cggccgtctt cgtgatgcct gcgttgttga tcagggcgtc gagcccgccg agcgcctctg
       61 cggcggcgtc cacggtcttc tcgcactccg cggtgttgct caggtcgccc tgaatagtgg
      121 cggcccgtcc cccgagttgc tctatgcggg agacggtctc atccggctcg gagtgggcgt
      181 agtggacggc cacctcggcg ccctggcggg cgagctcgac ggcgatgccc tggcctatgc
      241 ccatgccggc gccggtcacc agcgccttct tgccttcgag ggctctcacc cgtcggcccc
      301 cgttctggcg ccctcgcgtt tctcccagcc ctcggagaag aggttgaagt ggacgttctg
      361 tttcgggtgg gccgctatca cgtcttcgtc cagctcgacg ccgaggcctg gtcccttcgg
      421 gagctcgaag tagccgtcca ctacttccgg gaggcctggg gccgtctccc ttacccaggg
      481 gtcggcgaag tcgttgaagt actcctgaat gcggaagttc gtggtcgcgg cggccagatg
      541 cagggctgcg gcggtgttga tctgcccccc cacgttgtgc ggcgcgatgg tcatgtaata
      601 agcgtcggcc caggccgcga gcttcttggt ctccagcaac ccgccgatgt ggcttatgtc
      661 gggctgtatg acgtccgcgg cctgcagctc gaagacctcc ctgtactcca ggcgctggtg
      721 gatgcgctcg cccgtggcga cggtgtcctc tatgccgtcg gaggccttct tcagggcctt
      781 gaggttgacc ggcggcaccg gctcctcgac ccaacccagc ccgaagggcc tgagcatgcg
      841 cgccatctcg atagccgtgg ccggggagaa gcggccgtgc atctcgagca ggatctcgac
      901 ctctggcccg atggcgtccc tgacggcctc gactagcgag acggcccgaa gcttctcctc
      961 gtactccagc tcgtagtggc cagggccgaa gggatccagc ttcaatgccc tgtacccgcg
     1021 ctccacgacc ttcttcgccg cctcgtggaa ctcctcgggc gttcgctcca ccctgtacca
     1081 gccgttggcg taggccttga ccctgtccct gacggagccg ccgagcaggt tgtagaccgg
     1141 ctggcccagt gccttgccca cgatgtccca gcaggcggtc tcgaacatgg cgatgccgct
     1201 gctcataacc tcgccggccc gcgagaagtc gtcgcggccc atcttcctga ccagctcctc
     1261 tatcctgaag gggtcgtgcc cgatgaggtg cctcggggca gcctcggcca gataaccgac
     1321 gagcgcgtcc gtgtggttga ccatcctcgc ttccccgagg ccggttagcc cctcgtccgt
     1381 gtgggcccgg atgatggtca ggttgcgcca ctccgtaccg acgacgaagg tgtcgaggtg
     1441 cgtgatcttc atgctttctc cttcacttcg gcgtccgtag gtaggccggg aaaaacagcg
     1501 cctggagggg tggtctcggc gtcgggatag taacgcgcca gcatctcctg cccgattctg
     1561 gcccgcctga cccgctccct gccgagcgcg acggccgtcg cctccaggtg cgtgccgctc
     1621 gggtagatgt tgggcgcgtt cctcaggccg aacttgacga aaacgggcga ggctaccctg
     1681 acgaggtccg gtatctcgta gtgcctcacg aaccccccga agtcgtctgg cacctcgacg
     1741 tagacgtcga gtggcaggtc ggccacggct cgtatggcag ccagctgcgc catcgaaagg
     1801 tcggttggca cgttatacgt gtcggccccg aggtcctcca tcgccttcac ggctgccgcg
     1861 ttggccgcgg cgagctgcac ggagaccttc aggacgaggt cctccggcag ctccgctttg
     1921 gccttgaggt cgcgcaggac ccagagcagc cccgcgtcgg cgaccaggac cccgcgcaga
     1981 cccagggaga cgccacgtag cacgtcttcc agggcgtagg cgagctggtc ctgcccccgg
     2041 tgctgggcgc cgaggacctt gcctgcgccg gagatcgccg tcgcgccggt ctcccacgag
     2101 gcgcgcggcc ccacgaagag cgagagctcc agggcggcct cgcgcgccac ctggcacatc
     2161 tccaggatct cggcgtcggt gagcagcatg atccccgacc cctgggagac gcggtgtatc
     2221 tgtacttccc gtttcctggc ctcgtcgata accgcctcga gcacccgcgg tccctcgacg
     2281 ctgggtatct ctaccctgta ctgcgccccg tcgggaaagc gtttgcccga gtcgggtgga
     2341 tcgtggagat cccgccccgg catcccgagc ccggatagta gttcgtgtgc cctcataccc
     2401 cctccttctc cttgaattcc ccggtgtcga gcatcccctc gccgattgcg gcctcgatgt
     2461 cttctgaggt cttggcgaga tgcgcccgca tctcccgtcg cgcccccgcg gcgtccccgg
     2521 cctccacgtg gcggagtatc tgttcgtgct ccgcgagggc ccgctgcgcg ttgccaggcc
     2581 tggccgcgct gacccggcgg ctcgcccgaa gcaactcgac cacaggctct agcatggtga
     2641 gcaagacctc gttgcgtgcc ccctgcgcga gcagcgcgtg gaactcgacg tcggcgtcca
     2701 cgtagccctc gggcgcctcg atggaccgcc gcatcc
//
LOCUS       DBNGVD010000322         4987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_619825, whole genome shotgun sequence.
ACCESSION   DBNGVD010000322 DBNGVD010000000
VERSION     DBNGVD010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_619825"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16CM_08640"
     CDS             <1..266
                     /locus_tag="AC16CM_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139939.1"
                     /translation="GVMLTYGSGWGTVALAQGPQKGGASVPGLQAAGNDAALPKVDLG
                     GVEATELSTPVGTGLSWNADGVSYALAGSVPASELEQAARTLR"
     gene            270..1250
                     /locus_tag="AC16CM_08645"
     CDS             270..1250
                     /locus_tag="AC16CM_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139940.1"
                     /translation="MVEASDQRAPSVGTDGVPALELEGLTKRFGDFVAVEDLSFVVRR
                     GDVYGFLGPNGSGKSTTIRMILGLVRPTSGEIRVFGHPATSPEERAGMAGFVEAPGFY
                     EYLSARDNLRLLAAADRRAEGRAPLREVLETVGLSERARDKVRTYSQGMKQRLAIAAA
                     LLREPEFLVLDEPTNGLDPGGMRDIRALVRQLSGEGLTIFLSTHLLSEVEQLCNRVAV
                     VSRGRLLAEGALAEIAEQARDRTEYRLVVDDPERTRTVLNSREDVALLNGRPEELEGF
                     RPEEVVRFSTGAEGAGPVVRGLVEEGVEVLALVPVRPNLEELFLRLTEDD"
     gene            1250..2050
                     /locus_tag="AC16CM_08650"
     CDS             1250..2050
                     /locus_tag="AC16CM_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139941.1"
                     /translation="MVRWELFKLIRQRRTFLGLGAAAVVPILFLIAVRITREGPPPGE
                     APFAAQLLSNGLVIPLITLAFGSVVLLPLLTVLVAGDAIAGEVSAGTLKTMLGRSVSR
                     SGLFWAKAVAVEVYALCVVVVFVGVGMLAGWLAVGFEPLAGLGGTPISVGEGIGLIWA
                     SAGLVALPLLALAAFAIFLSSVTHNSVASVVGGVVLILLLQLVSTLGLGGLLDPYLLT
                     NQFSAWLNLMRDPIDWDPIVRAAWVSLLWAAASFLAAWAVFVRRDVLS"
     gene            2167..2487
                     /locus_tag="AC16CM_08655"
     CDS             2167..2487
                     /locus_tag="AC16CM_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3139942.1"
                     /translation="MEARSVAGSEAFSGEKMQKNALFDSPHMFYDTYCLLPGQAQKVH
                     VHEDSDKVYYVLRGTGRFIVAEEERDLGEGHAVIARAGDSHGVRNETQEELVLLVTMA
                     PRPS"
     gene            2506..2859
                     /locus_tag="AC16CM_08660"
     CDS             2506..2859
                     /locus_tag="AC16CM_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139943.1"
                     /translation="MPRQPYVLLFDEVVPGATPLTILVDDRGAVPLFDSEDKAGDFLA
                     STDFGAEFEAVEVSGAGLARALEAVEREVGYVAINPPPAGEGPMRVRMGGLTELVEAL
                     RQNPEDDLFDLRGLS"
     gene            complement(3013..3570)
                     /gene="msrA"
                     /locus_tag="AC16CM_08665"
     CDS             complement(3013..3570)
                     /gene="msrA"
                     /locus_tag="AC16CM_08665"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3139944.1"
                     /translation="MMENNGSSHVEVATLGGGCFWCLEAVYQELRGVEKVESGYSGGD
                     VPNPTYRQVCSETTGHAEVVQVTFDPNEVSYRDILDVYFTIHDPTTLNRQGADVGTQY
                     RSAIFYHSEEQKSVAEETISDLETQGIWNNPIVTEVVPFDEFYVAEDYHQDYFRNNGF
                     QPYCQVIIAPKVAKFRKQNLERLRG"
     gene            3710..4153
                     /locus_tag="AC16CM_08670"
     CDS             3710..4153
                     /locus_tag="AC16CM_08670"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR3139945.1"
                     /translation="MAPREHIKRAVQKIREVWRHWRTFREIDAGHRYQARYHLHQRRT
                     RRGLVSRYWKPVSLICGPILIFAGFLFIPTPGPSYLIIAIGMWLLAGEILALARIFDK
                     VGVRLGRVWHRIKDLWHALHVVVKVMIVCALAILLIHTVYSLFDG"
     gene            4236..4658
                     /locus_tag="AC16CM_08675"
     CDS             4236..4658
                     /locus_tag="AC16CM_08675"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR3139946.1"
                     /translation="MIDSVKRFAWQQWRSYSRGRPGKRFQERYRRRQESGPGKWSVSR
                     ILNLTLGILIVAVSAVGGLLPGPGWGTAIIGFALIAGEFHPAARLLDRMELALRAFLQ
                     LLRDTWGHSWAGKALIIIATAVIVFILAYTTYALLFAG"
     gene            complement(4703..>4987)
                     /locus_tag="AC16CM_08680"
     CDS             complement(4703..>4987)
                     /locus_tag="AC16CM_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HIR3139947.1"
                     /translation="GLYCTPTSKPCYYYASDNLQSPAYKGLTPDDLVDIAVDNGLHFD
                     GASQQGVVFHLIGALSQFGKVGTVCIGDSHESALKYYEDTVAVLDREAQR"
BASE COUNT          809 a         1544 c         1671 g          963 t
ORIGIN      
        1 acggtgtgat gcttacctac ggctcgggat ggggaaccgt ggccctggcc cagggtccgc
       61 aaaaaggcgg cgcatcagtt ccagggctcc aggcagccgg taacgacgcc gcgctaccga
      121 aggtggatct cggtggcgtc gaggccaccg agctctccac ccccgtgggc acggggctaa
      181 gctggaacgc ggacggggtc tcctacgccc tggccggcag cgttcccgcc tccgagctgg
      241 agcaggctgc ccggacgctc cgctagacct tggtcgaagc ctcagaccag agagcgccgt
      301 cggtcggcac cgacggcgtt cccgcgctcg agctagaagg cctgaccaag cgtttcggcg
      361 actttgtcgc ggtagaggat ttgagcttcg tggtcaggcg gggggacgtc tacgggtttc
      421 tgggacccaa cggcagcggg aagagcacga ccatacgcat gatcctcggc ctcgtcaggc
      481 ccacgtccgg tgagataagg gtgttcggcc atccggccac ctcgccggag gagcgggcgg
      541 ggatggccgg cttcgttgag gcgccgggtt tctacgagta cctctcggcc cgcgacaacc
      601 tgaggctcct ggcggcggcg gaccggcggg ctgagggtag ggcgcccttg cgggaggtgc
      661 tcgagacggt agggctctcg gagagggcca gggacaaggt gagaacctat tcccagggca
      721 tgaagcagcg ccttgccata gccgctgccc tgctcaggga gccggagttt ctcgtcctgg
      781 acgagcccac gaacggcctg gatcccgggg gcatgcgcga tatcagggcg ctcgtcaggc
      841 aactctccgg cgagggcctg acgatcttcc tctccaccca cctgctctcg gaggtcgagc
      901 aactgtgcaa ccgggtcgcc gtcgtgagcc ggggccgtct tctggccgaa ggtgcgctgg
      961 cggagatcgc cgagcaggcc agggaccgca ccgagtaccg cctggtggtc gacgacccgg
     1021 agaggacgcg tacggtcctg aattcaaggg aggacgtggc tctgctgaac ggtcgacccg
     1081 aagaactcga aggattcagg ccagaggagg tggtccggtt ctcgacgggg gcggaagggg
     1141 ccggacccgt ggtcagaggc ctggtcgagg aaggcgtcga ggtgctagcg ctggtgcccg
     1201 tccgtcccaa cctggaggag ttgttcttgc ggctgacgga ggacgactag tggttcgctg
     1261 ggagctgttc aagttgatcc gccagcggag gaccttcctc gggctggggg ccgccgccgt
     1321 cgtcccgatc ctgttcctga tcgcggtcag gatcacacgg gagggtccac cgccggggga
     1381 ggcgccgttc gccgcccagt tgctctccaa cggcctcgtc atacccctca taacgctggc
     1441 cttcgggtcc gtggttctcc taccgctgct gaccgtcctc gtcgccgggg acgccatagc
     1501 gggggaggtg tcggccggca cgctgaagac catgctcggc cgctcggtga gccgctcggg
     1561 actgttctgg gccaaggccg tggccgtcga ggtgtacgcg ctgtgcgtgg tggtcgtctt
     1621 tgtcggggtt ggaatgctgg cggggtggct ggcggtgggg ttcgagccgc ttgcgggcct
     1681 cggcgggacg cccatctccg tcggggaggg catcgggctc atatgggcca gcgccggcct
     1741 cgttgcgctg cctctcctgg ccctcgcggc cttcgccatc ttcctctctt ccgttaccca
     1801 caacagcgtc gcgtccgtgg tcgggggggt cgtcctgatc ctgctcctcc agctcgtctc
     1861 caccctgggg ctgggcggcc tccttgaccc ctacctgctg accaaccagt tctcggcctg
     1921 gctcaacctc atgcgcgacc ccatagactg ggaccctatc gtcagggcgg cctgggtgag
     1981 cctcctgtgg gcggccgcct ccttcctggc cgcctgggcc gtgttcgtcc gcagggacgt
     2041 ccttagctga agcacgctgt cagctttttg tcgacaccgg ccggctgagg gcgtgctagg
     2101 aggatgatcc catttgtgcg ctgctgtaga gtggggctcc ctgttatcag agtgaaggag
     2161 tagtgtatgg aagcgagatc cgtcgcaggc tcggaggcgt tctccgggga gaagatgcag
     2221 aagaacgccc tgttcgactc accacatatg ttctacgaca cctactgtct gctcccaggc
     2281 caggcccaga aggtccacgt ccacgaggac tcggacaagg tctactatgt gctgcggggc
     2341 accggtcggt tcatcgtggc cgaagaggag cgggatctcg gcgagggcca cgccgtgata
     2401 gccagggccg gagactccca cggcgtccgt aacgagacac aggaggagct ggtcctcctg
     2461 gtgacgatgg cccccaggcc ctcctgaccc gtggcctcgg aggagatgcc gcgccagccc
     2521 tacgtgctcc tcttcgatga ggtcgtgccc ggtgccacgc ccctgaccat cctcgtagac
     2581 gaccgcgggg ccgtgccgct cttcgactcg gaggacaagg ccggagactt cctggcttcg
     2641 acggacttcg gcgcggagtt cgaggcggta gaggtctcgg gtgccgggct cgccagggcg
     2701 ctcgaagccg tggagcgcga ggtgggctat gtcgccatca acccgccgcc cgccggcgag
     2761 ggccccatga gggtccgaat gggcggcctg acggagctgg tcgaagccct gcgccagaac
     2821 ccggaagacg acctcttcga cctgcgcggg ctttcttaga gccttcagct gatagctgtc
     2881 agccgtcagc tatttcacgc cttcaggaga gtttctgtcc tgtcgcccgt tgctcgatgc
     2941 tctcatccac atctggtgcc gcttcgagac caacaagctg atcgctgata gctaatagct
     3001 aacggctgac agctaacccc tcaggcgttc caaattctgc ttgcggaact tggcgacctt
     3061 gggagcgatt atcacctggc aatatggctg gaacccgttg tttcggaagt agtcctgatg
     3121 gtagtcctcg gcgacgtaga actcgtcgaa tgggaccacc tccgtcacga tggggttgtt
     3181 ccagatccct tgcgtctcca ggtcggagat cgtctcttcg gcgacgctct tctgctcttc
     3241 tgagtggtag aagatcgcgg acctgtattg tgtccccacg tcagcaccct gacggttcag
     3301 ggtcgtcggg tcgtggatgg tgaagtagac gtccaggatg tccctgtagg agacctcgtt
     3361 ggggtcgaac gttacctgca ccacctcggc gtggcccgtc gtctcggagc aaacctgcct
     3421 gtaggtcggg ttgggaacat cgcccccgga gtacccggac tccaccttct caacgccgcg
     3481 caactcctgg taaaccgcct ccaggcacca gaagcagccg ccgcccagcg tcgcaacctc
     3541 cacgtgggat gaaccgttgt tctccatcat ccttctttcc gtatgttttc tacggtttcg
     3601 gtataccgca cgcgacggat gcgtgcacgg tggtcgcgca cattggagag acgatggtct
     3661 gcgccggtta ccccttgctt ttcggcgggg cgttgttcta actttgccgg tggcccctcg
     3721 ggagcacatc aagcgcgccg ttcagaagat cagggaagtc tggcgtcact ggcgcacctt
     3781 ccgggagatc gacgcggggc accgctacca ggcccgctac catctgcacc agcgtcgtac
     3841 caggaggggt ctggtatcaa ggtactggaa gcccgtgagc ctgatatgcg gtcccatact
     3901 gatcttcgcc ggcttcttgt tcatcccgac gcccgggccc agctacctga tcatcgccat
     3961 cgggatgtgg ctgctcgccg gagagattct ggccctcgcc cgcatcttcg acaaggtggg
     4021 ggtcaggctt gggagggtct ggcataggat caaagacctg tggcacgctt tgcacgtcgt
     4081 agtgaaggtc atgatcgtct gcgccctcgc catcctgctg atccacaccg tctattccct
     4141 gttcgacggt tgatggcacc ccgaggatgg tgcaacctgg gagcgctttt cgcgtacgta
     4201 aaaacgagag cgcacagaag agaggcacca tcaggatgat cgatagcgta aaacggttcg
     4261 cctggcagca atggcggagt tacagtcgag gcaggcctgg caaacgcttc caggagcgtt
     4321 accgaaggcg ccaggagagt ggtcccggca agtggagcgt gagccgcatc ctgaacctca
     4381 ccctgggcat cctgatcgtg gccgtgagcg cggtaggtgg cctgctcccc ggcccgggct
     4441 ggggaacggc gatcatcggg ttcgccctga tcgcgggcga gtttcatcct gccgcacgcc
     4501 tgctcgaccg gatggagctg gcgctgcggg cgttcttgca gctcctcagg gacacctggg
     4561 gccattcctg ggcagggaaa gcgctcatca tcatcgcgac cgcggtaata gtcttcatcc
     4621 tggcctacac gacctacgcc ctgctcttcg ccggttagag ccttcgggga tcaaccagat
     4681 gcaagaagct aatagctgag agctatctct gcgcttcgcg gtcgaggacg gcgactgtgt
     4741 cctcgtagta tttcagggcg ctctcgtggc tgtcgcctat gcagacggtg cccaccttgc
     4801 cgaactggga gagggctccg atgaggtgga agaccacacc ctgctgggag gcaccgtcga
     4861 agtggaggcc gttgtccacg gcgatatcaa ccaggtcgtc tggggtcagg cccttgtaag
     4921 cggggctctg gaggttgtcc gaggcgtagt agtagcaggg tttcgacgtc ggggtgcagt
     4981 agaggcc
//
LOCUS       DBNGVD010000323         3237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2077899, whole genome shotgun sequence.
ACCESSION   DBNGVD010000323 DBNGVD010000000
VERSION     DBNGVD010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2077899"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC16CM_08685"
     CDS             complement(<1..962)
                     /locus_tag="AC16CM_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139948.1"
                     /translation="MTETTKDQEKTIGGPERHRLPAVVRIAGWLIVPLYLVSDLAYYI
                     LTYSATPQGDSSLIDQVEGFALDLGFSTFAAVGALLVIRRPYNAIGWIMASIGLMVPV
                     FNSGTAYALYVMVHRGRPDTLAVFGAWAGNWYWFVMLSLALIYLPMLFPDGRLLSRRW
                     LPVAVLGGSGALGVAILGALTDTLVVGNGPRFEIDNPIGVEGLAAPWTLPVFVVFEIL
                     FAIGVGGAAASVIVRLKRSRGVERRQLEWFAYVTALFFGAAMITGIVSSVAGVGWLDE
                     ISFATSLIGLVCLPIAVGVAVLKYRLYDIDIIINRTLVYGSLTLL"
     gene            complement(1039..1305)
                     /locus_tag="AC16CM_08690"
     CDS             complement(1039..1305)
                     /locus_tag="AC16CM_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139949.1"
                     /translation="MSAGDHAARQEAKRRLSEPEGDHELHAAMEGMKERHPHLWNVLH
                     RVHFARDIDPSELDAWRRAPEGSPESDLVDHYDRALGYLGVALQ"
     gene            complement(1527..>3237)
                     /locus_tag="AC16CM_08695"
     CDS             complement(1527..>3237)
                     /locus_tag="AC16CM_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CASTOR/POLLUX-related putative ion channel"
                     /protein_id="HIR3139950.1"
                     /translation="TLWLGLMHTIDPGNVGGDTGGWSYLLTMLTVTAGGILVFSTLIG
                     VIFSGIDQKLADLRKGRSFVVERGHTVIYGWSPEVFPVVAELAKANESRRRSCIAILA
                     EEDKVEMEDQIRARVGKTGRTRVVCRTGDPINIEDIAIVNPNEARSIIVLPPEDEEAD
                     SRVIKTVLALTNNPDRPEKPYHIVSRIGRPENLGVARMVGRDELRLVAVDDLIARITA
                     QTCRQSGLSVVYTDLLNFGGDEVYFDHEPRLVGLTFGEVLVSYEACAVIGLRTREGRT
                     LLNPPSETEVSVGDEIILIAEDDSTVRLSEGPAPEIDAGAIRETPPYEPAPERTLILG
                     WNNRAPKIVSELDHYVRPGSEVTVVTSDVACPPSALGVLENQRLSYRAGDVTERHVLD
                     GLGVAEYDHVVVLGDADGSDANAADAKTLVTLLHLREISERGGGRFSTVSEMLDVRNR
                     ELAEVTGADDFIVSDHLASLMMCQVSENRDLSTVFEGLISAEGSELYLRPATDYVKPG
                     VPLNFYTVVEAARRRGETAVGYRLAEETANPAASYGVYLNPEKSRLITFSGHDRLIVL
                     AES"
BASE COUNT          584 a         1043 c         1048 g          562 t
ORIGIN      
        1 aggagcgtga gcgagccgta gacgagcgtg cggttgatga ttatgtctat gtcgtagagg
       61 cggtacttca ggaccgccac gcctacggct atgggcaggc acaccaggcc gatcaagctc
      121 gttgcgaacg agatctcgtc gagccatccc acgccggcca cgctcgagac tatgccggta
      181 atcatggcag cgccgaagaa cagggcggtg acgtaggcga accactccag ctgccggcgc
      241 tccaccccgc gcgagcgctt caagcggact atcacagacg ccgccgcgcc gccaacgccg
      301 atggcgaaca ggatctcgaa cacgacgaac accggcaggg tccagggcgc ggccaggccc
      361 tccaccccga tcgggttgtc tatctcgaag cgcgggccat tgcccacgac cagcgtatcc
      421 gttagtgccc ccaggatggc gacgcccagc gcgccagatc ccccgagcac ggcgaccggg
      481 agccagcggc gggagagcag gcgtccgtcg gggaaaagca tgggcaggta gatcagagcc
      541 agagagagca tcacgaacca gtaccagttc ccggcccacg cgccgaacac ggcgagggtg
      601 tctggcctac cgcggtggac catgacgtag agggcgtagg ccgtgccgga gttaaagacc
      661 ggcaccatca ggccgatgga ggccatgatc caaccgatgg cgttgtaggg acgtctgatc
      721 accagcagcg ctccgaccgc cgcgaaggtg gaaaatccca gatccagcgc aaagccctcc
      781 acctggtcga ttagcgatga atctccctgg ggagtagcac tgtaggtgag gatgtaatag
      841 gcgaggtcgc taacgaggta gagcgggact atcaaccatc ccgcgatgcg cacgacagcg
      901 ggtagccgat gccgttccgg cccgccgatg gtcttttcct gatcctttgt cgtctccgtc
      961 acgacaccag cttacggggg gaccgtctgc cagacgttac ggcaaccgtt acgacagagg
     1021 tttcgtagtc ccgggcggtt actgaagggc gacccccagg tacccgaggg ccctgtcgta
     1081 atggtcaacc aggtcactct ccgggctacc ctcaggcgcc ctgcgccatg cgtctagctc
     1141 ggaggggtcg atgtcgcgcg cgaaatgtac ccggtgaagt acgttccaga gatgggggtg
     1201 acgttccttc atgccctcca tggcggcgtg tagctcgtgg tcgccctcgg gctcggagag
     1261 cctccttttc gcttcctgcc tggccgcgtg atcgcctgcg ctcacgttcc cttctccctt
     1321 cgtcgggtgg cccatgatac caagcgcaaa agcccctacg cacctaagaa tcgggactcc
     1381 cagggacgac ggcagccatg tggacgtgta ccccgacaat tccttcaacg tgctcgacac
     1441 gccggacgac cccgggggac aggactcgcc tggaatccgc ttccgcggga atgatgggga
     1501 ggtgggaacg tgaggtcagg actccgtcaa gactccgcca ggacgatcaa cctgtcgtgg
     1561 ccggaaaacg tgatcagcct ggatttctcg gggttcaggt agacgccgta ggacgcggcc
     1621 gggtttgcag tctcttctgc gagccggtag cccacggccg tctcgcccct ccgtcgggct
     1681 gcctccacca cggtgtagaa gttcaggggc acgccgggtt tcacgtagtc tgtggcgggt
     1741 ctcaggtaga gttccgagcc ctcggcggag atcagcccct cgaacaccgt cgagaggtct
     1801 cgattctcgg agacctggca catcatgagg ctcgccaggt ggtcgctcac gataaagtca
     1861 tcggctccgg tcacctcggc gagctcacgg tttctaacgt caagcatctc gctgacggta
     1921 gagaaccggc ccccgcctcg ctcggagatc tcgcgcaggt gaaggagcgt gaccagcgtc
     1981 ttcgcgtcgg cggcgttggc gtctgaccca tctgcatctc cgagcaccac gacgtggtcg
     2041 tattcggcga cacccagccc gtcgagaacg tggcgctccg tgacgtcgcc agcgcggtaa
     2101 gagagcctct ggttctccag tactccgagg gcggacggcg gacaggcaac gtcagaggtc
     2161 accacggtga cctccgagcc gggacgtaca tagtggtcca gctcggagac tatcttcggc
     2221 gcgcggttgt tccagccgag gatcagggtg cgctcgggcg caggttcgta tggcggggtc
     2281 tcgcggatcg cgccggcatc gatctccggc gctggtcctt cggagagcct taccgtgctg
     2341 tcgtcctcgg cgatcaggat gatctcgtcg cccacggaga cctccgtctc cgacgggggg
     2401 ttgagtaggg tacggccctc cctggtgcgt agcccgatca cggcgcacgc ctcgtaggag
     2461 acgagaacct cgccgaaggt caggccaacg aggcggggct cgtggtcgaa gtagacctca
     2521 tccccgccga agttcagaag gtccgtgtac accacggaga ggcccgactg gcggcaggtc
     2581 tgggccgtga tgcgggcgat caggtcgtcg acggcgacga gcctcagctc atcgcgcccg
     2641 accatccgcg ccaccccgag gttctcgggg cggccgatcc tcgacacgat gtggtagggc
     2701 ttctcgggcc ggtccgggtt gttggttagc gccagcacgg tcttgatcac ccgcgagtcg
     2761 gcctcttcgt cctcgggagg aagcacgatg atagaacgcg cctcgttcgg attgacgatc
     2821 gcgatgtcct ctatgttgat tgggtctccc gtcctgcata cgacccgcgt cctgccggtc
     2881 ttgcccaccc tggcccggat ctgatcctcc atctcgacct tgtcttcctc ggccaggatg
     2941 gcgatgcacg accgcctccg gctctcgttg gccttcgcca actcggccac cacgggaaaa
     3001 acctcgggcg accagccgta aatgaccgtg tgcccgcgct cgaccacgaa cgaccggccc
     3061 ttgcgcaggt cggccagttt ctggtcgatg ccggagaaga tcacgccgat gagcgtgctg
     3121 aagaccagga tgccgccggc ggtgacggtc agcatggtga gcaggtagga ccagccaccc
     3181 gtatcgccgc cgacgttgcc ggggtctatg gtgtgcatga ggccgagcca gagcgtc
//
LOCUS       DBNGVD010000324         2500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4810576, whole genome shotgun sequence.
ACCESSION   DBNGVD010000324 DBNGVD010000000
VERSION     DBNGVD010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4810576"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="AC16CM_08700"
     CDS             complement(<1..1850)
                     /locus_tag="AC16CM_08700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3139951.1"
                     /translation="MQNPNVVRIGRRRRRSRNRSRLLQGLRTFFLLLCLMAVLVGAGM
                     VAGAVSTYSTLAANMPDLDDYRSTELAQTSLVYDANGRVVDQLYGVQNRFVVPLDEVD
                     PNLRKAIISLEDHRYYQHKGLDFEAIGRAAVENVASLSIQEGGSTLTQQLIKNTYIAQ
                     QQRLIPSFERKYVEASLAWQYEKGHSKKEILEQYLNTVYFGSNAYGAEAAARTYFNKD
                     ASDLTLSQSALLAGIINLPGVYDPFIDPESAKERRNVVLDKMLEYGQITKKEHDEAVA
                     SKLELSRGRIQYENDNEYFLDAVRREIAEEYGDDMLYEGGLEIHTTLDPDLQAKASEA
                     VGAVVNPEAGDPSAALVSVDPSTGAVRAMVGGSDFDQVKFNLATQAHRQAGSSFKPFS
                     YAEAVHEGISPETEYVSKHLSIDMGKNAEPYEVDNYDFIHRGPITLVKAMADSDNTVF
                     VQLALDLGLQKVVEMANKLGVDSEIDAYPATAIGGLRIGVTPLEMASAYSTFANGGTH
                     MEPFLVEKVTEDKNGDEVLLEKHRSIGEEVLSKDEAAVVTEALRAVVTRGTASYYHDL
                     DAEIGRPAAGKTGTSNEFIDAWFVGFIPQLSTSVWVGYPNERRSMVNINGL"
     gene            2001..>2500
                     /locus_tag="AC16CM_08705"
     CDS             2001..>2500
                     /locus_tag="AC16CM_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3139952.1"
                     /translation="MIEALREADDLCSGPAYLQGVGLFYRQAEYAARAREAAAKLVGA
                     GPDDVALTQNTTHGMNLGVSSLNWREGDEAISVATEHPGCLAPLHNLKRRHGVETRLV
                     EPPATPEKIEALLTPRTRLIALSHVDWTNGAVLPLEEICALARERGVMTLVDGAQSVG
                     NIEVDAP"
BASE COUNT          404 a          797 c          827 g          472 t
ORIGIN      
        1 aggccgttga tgttcaccat ggagcgccgc tcgttcgggt agccgaccca gacgctcgtg
       61 gagagctgcg gtatgaaccc gacgaaccag gcgtcgatga actcgttgga ggtgcccgtc
      121 ttgcccgcgg ccgggcgccc aatctcggcg tcgaggtcgt ggtagtagct cgccgtcccg
      181 cgggtaacga ccgcccgcag cgcctccgtt accacggcgg cctcgtcctt gctgaggacc
      241 tcttcgccga tggagcgatg cttctccaga agcacttcat ccccattctt atcctccgtc
      301 accttctcga ccaggaacgg ctccatgtgc gtgccgccgt tggcgaaggt ggagtaggca
      361 gaggccatct ccaggggggt taccccaatc ctcagcccgc caatggcggt cgccgggtag
      421 gcgtctatct cgctgtccac cccgagcttg ttggccatct caaccacctt ctgcaggccg
      481 agatccaggg caagctggac gaagacggtg ttgtccgagt cggccatcgc cttcacgagc
      541 gtgatcgggc cgcggtgaat gaagtcgtag ttgtccacct cgtagggctc ggcgttcttg
      601 cccatgtcga tgctgaggtg tttggagacg tactctgtct ccggagagat gccctcgtgc
      661 acggcctcgg cgtaggagaa gggcttgaac gagctgcccg cctgcctgtg ggcctgggtc
      721 gccaggttga acttcacctg gtcgaagtcc gagccgccga ccatcgccct gaccgcgccc
      781 gtggaggggt cgacggagac cagggcggcc gaggggtccc cggcctcggg gtttacgacg
      841 gcgcccacgg cctcgctcgc cttggcctgc aggtcggggt cgagggtggt atgtatctcg
      901 agccccccct cgtagagcat gtcgtcgccg tattcttcgg ctatctccct gcggacggcg
      961 tcgaggaagt actcgttgtc gttctcgtac tggatcctgc cacggctcag ctccagcttc
     1021 gaggcgacgg cctcgtcgtg ctcctttttg gtgatctgac cgtactcgag catcttgtcc
     1081 agcacgacgt ttcgcctctc cttggcgctc tcggggtcta taaaggggtc gtagacgccg
     1141 gggaggttga tgatcccggc caggagcgcc gactgggaga gggtgaggtc ggaggcgtcc
     1201 ttgttgaagt aggtcctggc ggcggcctcg gcaccgtagg cgttcgagcc gaagtacaca
     1261 gtgttcaggt actgctccag gatctctttt ttggagtgcc ccttctcgta ctgccaggcg
     1321 agcgaggcct ccacgtactt ccgctcgaag ctggggatga ggcgctgctg ctgggctatg
     1381 taggtgttct ttatgagctg ctgggtcagg gtggagccgc cctcctgaat ggagaggctc
     1441 gccacgttct cgacggccgc gcggcctatg gcctcgaagt cgaggccctt gtgctggtag
     1501 tagcggtggt cttcgaggga gataatggcc ttcctgaggt tggggtccac ctcatccagc
     1561 ggtaccacga agcggttctg tacaccgtag agttggtcta cgacccggcc gttcgcgtcg
     1621 tagaccaggg aggtctgggc gagctcggtg gagcggtagt cgtcgaggtc gggcatgttg
     1681 gcggcgaggg tactgtacgt gctcacggcg ccggcgacca tccccgcgcc gacgagcacg
     1741 gccatgaggc agagcagcag gaagaaggtc ctgagcccct gcaggagcct gctcctgttg
     1801 cgcgaccgcc tgcgacggcg gccgatcctg acgacgttcg ggttctgcat gaagcggcta
     1861 ttatacaaaa tgaccatcgt atccccgcaa aggaggagca cattacagcc cccagcctcc
     1921 agcgcgaagc gttccccacg ctcgccgcaa acgattttgc ctatctgaac acgggcagca
     1981 gcggacctcc cccctaccat gtgatcgagg ctttgcggga ggcagacgac ctctgctccg
     2041 gaccagccta cctccagggc gttggcctgt tctacaggca ggccgagtac gccgcgcggg
     2101 cacgcgaagc agccgcgaag ctcgtcgggg ccggccccga cgacgtggcc ctcacccaga
     2161 acaccaccca cggtatgaac ctgggcgtct cctccctgaa ctggcgggag ggcgacgaag
     2221 cgatctcggt cgccacggag caccccggct gcctggcgcc gctccacaat ctgaagcgac
     2281 gccacggggt agagaccagg ctggtcgagc ccccggcaac gccggagaag atcgaggccc
     2341 tgctgacgcc gaggacgcgc ctgatcgccc tctcacacgt ggactggacc aacggcgcgg
     2401 tgttgcctct ggaggagatc tgcgccctgg cacgcgaacg cggcgtgatg accctggtgg
     2461 acggcgccca gtcagtcggg aacatcgagg tcgacgcgcc
//
LOCUS       DBNGVD010000325         5300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2806564, whole genome shotgun sequence.
ACCESSION   DBNGVD010000325 DBNGVD010000000
VERSION     DBNGVD010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2806564"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16CM_08710"
     CDS             complement(<1..342)
                     /locus_tag="AC16CM_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3139953.1"
                     /translation="MVRVFVDGVQNSQEITHEQNLVNVEAETENFVRTMAGLGDRLGP
                     LLLQLPPSFTVEGMGVLEDFLNGPPEDFRYAVEVRHRSWLASDLPEMLRERGVALTLI
                     DYPRMPRMDQAT"
     gene            complement(548..1678)
                     /locus_tag="AC16CM_08715"
     CDS             complement(548..1678)
                     /locus_tag="AC16CM_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139954.1"
                     /translation="MARAVTLGLIPAPDIPEKIAQELASELPGLLSSRVDGGVSWDVV
                     VVVDPLTGTDREAPEILDECREKMLSEGWDLALCLTDLPVYRGGRLVAADVSSERGVG
                     SLSLPAMGALRLRPRSREATLRLIQELYEKVHEPEADATLKRSPRSTGFAGPFRRVDP
                     PDEDMAAMEVDARFAATGVLGRIGLWSGMVLANRPWGMLPAFKGAIAAAFATGAYALV
                     ITTLWVLADSVGWARLMLLMVTAIVAMVAWIIVAHHLWERPEEPDQQKWAALYNGVTV
                     LTVTSAVLCAYAILFTLILLAAWVFVPGDYFQTILKHPVGFGEYLTLSWLAASLATVA
                     GALGASLEDEETVRKASYGYRQRRRHVDENPEETSRDGLEER"
     gene            complement(1816..4488)
                     /locus_tag="AC16CM_08720"
     CDS             complement(1816..4488)
                     /locus_tag="AC16CM_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3139955.1"
                     /translation="MATRMCDVCGIRPAVVTVRRVVPGEGQRVEHLCEIHAAEAGVGR
                     STFGGRSPLGGGSLFDDFFDRFFEEEPRGGGVVPRRQAEQVDITQFFNESTTELLQRA
                     AGQAVEWGNLDLSSEHLLYAALEDNVVRRVLEGVEADPEAIRAQLEEEAEKGGRTNVT
                     PSLAPDAKRALLAAYEESQALGSSYIGPEHVLLALARDDESEAGELLGRFGLSHTRLR
                     GAVMRGVDTATGAAREPASATPTLDEYSRDLTQMAREGKLDPVIGRAEEVETTIEILS
                     RRTKNNPVLIGDPGVGKTAIVEGLAQRIVNDEVPETLVGRRVLALDLSGLVAGTQYRG
                     QFEERLKQVVDEASENPEGQILFIDELHTVVGAGAAEGSMDASNMLKPALARGELHVV
                     GATTVDEYRKNVEKDAALERRFQPVLVGEPSVDDTIDILRGLKDRYEAHHRVKITEEA
                     IVAAAELSDRYVTDRFLPDKAIDLMDQASARVRLRSKTKPRDTKELEEEVARLGREKD
                     QAVSAEDFGRAQELKGRVENLRFELDGSRDGARRPVAKVTAEDIAEVVSRATGIPVSQ
                     LTQEERARLMRLEEQLHERVVGQDEAVTAVAEAIRRARAGLSDPNRPIGSFLFLGPTG
                     VGKTELARTLAEALFGDEAAMVRIDMSEFQERHTVSRLVGAPPGYVGYEEAGQLTESV
                     RRRPYSVLLLDEIEKAHPDVFNILLQILDDGRLTDAQGRTVDFKQTVIIMTSNMGAQR
                     IQAHARRDESFEELKDDMMQILKNTLRPEFVNRIDEVIVFRSLGKEQIGEIARLLLDR
                     TTRRLRAQGIEVEFTEGAVGLIGEEGFDPEFGARPLRRTIQRRVDNELASMVLSGALD
                     PGDRVVVGTDGEAGGLTFEVVAGATAAESPGEEG"
     gene            complement(4646..>5300)
                     /locus_tag="AC16CM_08725"
     CDS             complement(4646..>5300)
                     /locus_tag="AC16CM_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3139956.1"
                     /translation="ASLFTAVLILTIMPIYLAAQPEPAVGWFIRFFPPDRRDRVREVL
                     SKVRSGLLLWLKGRIADMAIVGILSTVALYLIGVPGALFLGVLTGLLAFVPLVGSIVS
                     AIPPLLLAFVVGPTTVLWVLVAYVAIQQIEGNLVEPLIMQKTASLHPAAVVTVVTVLG
                     AAFGVLGSILAVPSAIVAAILVKELWFRRLEEARTEPNRNAGNEGTPEKPRQTVRDA"
BASE COUNT          880 a         1803 c         1621 g          996 t
ORIGIN      
        1 cgtcgcctga tccatcctgg gcatccttgg atagtcgata agcgtcaggg cgacgccgcg
       61 ctcgcgtagc atctccggaa ggtcggacgc gagccagctt cgatgcctta cctcgacggc
      121 gtatcgaaag tcctccggag gaccattcaa gaaatcctcg agcacgccca taccctccac
      181 ggtgaaggag ggcggcagct gaaggagaag gggcccgagc ctgtcgccga gaccggccat
      241 ggtgcgtaca aagttctcgg tctcggcttc gacattgacc agattctgtt cgtgggttat
      301 ctcttgggag ttttgtacac catccacaaa cactctcacc atcgtggccg taaaaggatg
      361 agtatagcgc caagcttata cagtgaggtg gcccaaggcg ctccgctata gtcctcgggc
      421 gcccggcctt cctaatctcc taatgcacct aacgaactag aagaggagtt ctgcgaactt
      481 tgcccaaacg gcgttctatg aagttcggta tggcctcgga gtccaaaccg ggagcgctcc
      541 ttaggcttca ccgctcttcg agaccgtccc gtgacgtctc ctcgggattt tcgtcgacat
      601 gacggcgcct ctgacggtag ccgtaggatg ccttgcgcac cgtttcctcg tcttccaggc
      661 tggcccccaa ggctcccgcg accgttgcca gggaggccgc aagccaggag agcgtcaggt
      721 actccccgaa gcccaccggg tgcttgagga tggtctggaa gtagtcgccc ggcacgaaca
      781 cccacgccgc cagcaggatc agggtgaaca ggatggcgta ggcacacagc accgccgacg
      841 tgaccgtcag cacggtcacc ccgttgtaga gagctgccca cttttgttga tccggctcct
      901 cggggcgctc ccacaggtgg tgggccacga tgatccaagc gaccatcgcc acgattgccg
      961 tgaccatcaa cagcattaga cgcgcccaac ccacggagtc ggccagcacc cagagggtgg
     1021 tgatcaccaa cgcgtaggcg cccgtggcga aggcggcggc tatggcgccc ttgaaggcgg
     1081 gaagcattcc ccaggggcgg ttggccagaa ccatccccga ccagagtcct atgcgcccga
     1141 gcacccccgt tgcggcgaag cgagcgtcga cctccatcgc cgccatgtcc tcgtctgggg
     1201 gatcgacccg ccggaagggc cctgcgaaac cggtggagcg tggacttcgc ttgagagtgg
     1261 catccgcttc gggctcgtgc accttctcgt agagctcttg gatgagcctg agggtggctt
     1321 ctcgggacct cggacgcagt cgcagcgcgc ccatggcggg cagcgagagg ctccccacgc
     1381 cgcgctcgga gctgacgtcc gccgcgacga gccgcccgcc cctgtagacc ggcaggtccg
     1441 tcaggcagag ggccaggtcc cagccctcgc tcagcatctt ctcccggcac tcgtccagta
     1501 tctccggcgc ttcccggtcg gtgccggtga ggggatcgac cactaccacc acgtcccacg
     1561 agaccccccc gtcgacccgg ctgctcaaga gaccgggcag ttcggaagcc agctcctggg
     1621 cgatcttctc ggggatgtct ggggcgggta tcagtcccag cgtcacagct cgggccacga
     1681 acacctcctc gcagtgcctg tcgtaaggag tacttccaca gtcctaccac tcgcgtagcg
     1741 gggttaaacc gagggctcgg tcgtggtcca cggttggcgt tatgccctat tcagcagagt
     1801 acgtggaagg agcgttcacc cttcctcacc cggggactcc gccgcggtcg cccccgcgac
     1861 gacctcgaag gtgagaccgc ctgcttcgcc atcagtgccg acaaccaccc tgtcgccggg
     1921 atccaaggct cccgagagga ccatgctggc cagctcgttg tccacccgcc gctgtatcgt
     1981 acgcctcaag ggcctcgccc cgaactcggg gtcgaagccc tcttcgccta tcagcccgac
     2041 cgccccttca gtgaactcga cctctatgcc ttgagccctc aggcgcctag tcgtccggtc
     2101 gagcaggagg cgagcgatct cgcctatctg ctccttgccg agggagcgga agacgatgac
     2161 ctcgtctatg cggttgacga actcgggacg cagggtgttt ttgaggatct gcatcatatc
     2221 gtccttgagc tcctcgaagg actcatcacg cctggcgtgc gcctgtatcc tctgtgcgcc
     2281 catgttggag gtcatgatga tcaccgtctg cttgaagtcc accgtccggc cctgcgcgtc
     2341 cgtcaggcgt ccgtcatcca ggatctgcag gaggatgttg aagacgtccg ggtgggcctt
     2401 ctcaatctcg tccaggagca agactgagta gggcctcctc ctaacgctct cggtgagctg
     2461 gccggcctcc tcgtagccca cgtagccggg aggggcccca accagacggc tcacagtgtg
     2521 ccgctcctgg aactcgctca tatctatgcg caccatcgcc gcctcgtctc cgaagagggc
     2581 ctcggccagc gtgcgggcga gctcggtctt gccgacgccc gtgggaccca agaacaggaa
     2641 ggagccgatc gggcggttgg ggtccgagag tccggccctt gccctgcgga tagcctcggc
     2701 aacggccgtg acggcctcgt cctgccccac caccctctcg tgcagctgct cctcaagacg
     2761 catgagccgc gcgcgctcct cctgggtgag ctgggagacg gggatgccgg tggcgcggct
     2821 caccacctcc gctatgtcct cggccgtcac cttggcgacg gggcggcggg cgccgtcgcg
     2881 cgacccgtcc agttcgaacc tcaagttctc gacccggccc ttgagctcct gggcccttcc
     2941 gaagtcctcg gccgagaccg cctggtcctt ctctcggccc aggcgcgcca cctcttcctc
     3001 gagctccttc gtgtcgcgtg gtttggtctt ggagcgcagc ctgaccctcg ccgaagcctg
     3061 gtccatgaga tctatggcct tatcggggag gaagcggtcc gtgacgtagc ggtccgagag
     3121 ctcggcagcg gccacgatgg cctcctccgt tatcttcacc ctgtggtggg cctcgtagcg
     3181 gtcctttagg cccctgagga tgtctatagt gtcgtccact gagggctcgc ccaccaggac
     3241 cggctggaag cggcgctcta gggcggcgtc cttctctacg ttcttgcggt actcgtcgac
     3301 ggtggtcgcc ccgacgacgt gcagctcgcc ccgcgccaaa gcgggcttga gcatgttgga
     3361 cgcatccatc gagccttctg ccgccccggc tcctaccacc gtgtgcagct cgtctataaa
     3421 caggatctgc ccctcgggat tctcgctcgc ctcgtctacg acctgcttga gacgctcctc
     3481 gaactggccg cggtactggg tgcctgccac cagcccggag aggtcgaggg ccaagaccct
     3541 cctgccgacc aaggtctccg gcacctcgtc gtttacgatc ctctgggcga ggccctccac
     3601 gatggctgtc ttgccgacgc cggggtcccc gatgagcacg gggttgttct tggtccgcct
     3661 ggagaggatc tcgatggtgg tctctacttc ctcggcgcgc ccgattacag gatcgagctt
     3721 gccctcacgg gccatctggg ttaggtcgcg gctgtactcg tcgagggtgg gtgtggcgct
     3781 ggccggctcg cgcgcggcgc ccgtggcggt gtctaccccg cgcatcacgg cccccctgag
     3841 cctcgtgtgc gagagcccga aacgacccaa cagttcgccc gcctccgatt cgtcatctct
     3901 cgccagcgca agcaggacat gctcaggacc tatgtaggag gagcccagag cctgcgactc
     3961 ctcataggcg gctaagagcg cccgcttggc atcgggggcc agggaggggg tgacgttggt
     4021 gcgcccgcct ttttcggcct cctcctcgag ctgggcccgg atcgcttcgg ggtctgcctc
     4081 cactccttcg agcacgcgcc gcaccacatt gtcttcgagc gccgcataaa ggaggtgctc
     4141 gctactaagg tctaggttgc cccactcgac ggcttgcccg gccgcccgct gcaatagttc
     4201 cgtcgtggat tcgttaaaga actgggttat gtccacctgt tctgcctgcc tccgggggac
     4261 taccccgccc ccgcggggtt cctcctcgaa gaacctgtcg aagaagtcat cgaagagact
     4321 tccgcccccc aggggagacc tgcccccaaa ggtcgaacgc ccgaccccgg cctcggcggc
     4381 gtggatctca cagaggtgct caaccctctg gccttccccg ggaacaaccc gcctcactgt
     4441 gacgaccgcc ggcctgatcc cgcagacgtc gcacatcctc gtggccatct cctcttgcct
     4501 ccttctcccg cctacttcgc gcggcctgtg ggtcgcgcac gttgtggcta ggtttggtta
     4561 ggggcgaaca acttgctcaa cgaggtggta tcggaaagta tttccatgta cgttttctat
     4621 ccttgctttc tgcgaggttg gtagcttatg cgtcccttac tgtctggcgg ggtttctcgg
     4681 gtgttccttc gtttcccgcg ttcctgttcg gttcagtcct cgcttcttcg agccgcctga
     4741 accacagctc tttgaccagg atcgcggcca cgatggccga cgggacggcc aggatgctgc
     4801 caagcacgcc gaaggccgcg cccaaaacgg tcacgaccgt cacaacggcc gccgggtgca
     4861 acgatgcggt cttctgcatg atcaacggct ccaccaaatt gccctcgatc tgctggatgg
     4921 ccacgtaggc cacaaggacc cacaggacgg ttgtcggacc cacgacgaag gccagcagga
     4981 gcggcgggat ggccgatacg atcgacccga cgagcggcac gaacgccagc aacccggtca
     5041 ggacgcccag gaagagcgcc ccggggacgc ctatgaggta aagggcgacc gtcgagagta
     5101 tgccgacgat ggccatgtcc gcgatccggc ccttgagcca caggagcagg ccggagcgga
     5161 ccttcgagag gacctcccgg accctgtccc tgcgatcggg cgggaagaac ctgatgaacc
     5221 acccgaccgc cggctcgggc tgtgccgcca ggtagattgg cattatggtc aagatcagca
     5281 ccgcagtgaa tagggacgct
//
LOCUS       DBNGVD010000326         7048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1531961, whole genome shotgun sequence.
ACCESSION   DBNGVD010000326 DBNGVD010000000
VERSION     DBNGVD010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1531961"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /gene="dnaG"
                     /locus_tag="AC16CM_08730"
     CDS             complement(<1..2630)
                     /gene="dnaG"
                     /locus_tag="AC16CM_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3139957.1"
                     /translation="MRIAQRSIEEVREAADIVEVTSEFTALKRQGARFSGLCPYPDHQ
                     EKTPSFSVSPDRGFYYCFGCLEANERIWTSRGLIPIAEAEPGDEVIGLDGSRETITDK
                     WFKSGPTLRIKTGAAKEGLQLTPDHHCVFVRKEEALRAIPRVHPRQSNGKKLRFSSRH
                     NPKNAHVMVSVDHASEVREGDFWLYPVISDEERDDSALQGQQVIKEYTRGPRNARIAE
                     LPVNPETAWLYGVWLAKGSLYRGGVKWSFSISEADTLAARVVETLKTEFGRPSTRFRR
                     PHKNLCEVTCSSTDLSTLFQYWFGRGCASKRLPVRALSWSTECQAALVQGYFDGESHT
                     SDGVTSAASVSEALTYGIFALCIQMGRVCSTSTMPERRKADGIWRRRTYYVHALSKES
                     LKGFFAEIDGTRYFWSIVQAVEPETEEPTTVVDITTTGSHTFLTKMGITHNCQRGGDT
                     IKLVMDLKSLDFAEAVSYIAERSGIELQFEGGGDPDAARQRAQRRREIHRALAAATVY
                     YHKYLLGSRTPEAEQARHYLRDRKIEHSTIEEFRLGYAPPRGVAGFLGTAKRLNLDRS
                     TLEAAGLLSGRGGERFAGRITFPISDRRGRIVGFGARTLGDAQPKYLNSPETDVFNKR
                     DLLYGFPQVSEAMRKERAALVVEGYTDVLMLYQSGIKNAVATLGTATTAGHLRMLSGY
                     ADRIYVLFDPDVAGERAIERAAATAAELKLDLRVLRLPEDPADWLLQHPPQEFAQLLE
                     GALPVLEYGIRRIVARTRGAGATERSRAVPEIRSMIGEIKDPVLYREALRLATESLGV
                     SHDVLIEKETTTDQSQAPSRRRRSDPHTEAGGAVLTLILTRPDLTAPLLKEGLRSNVL
                     SEPLMLRDEDFRDEVQARIFT"
     gene            2735..5530
                     /gene="ppc"
                     /locus_tag="AC16CM_08735"
     CDS             2735..5530
                     /gene="ppc"
                     /locus_tag="AC16CM_08735"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3139958.1"
                     /translation="MVPRAGYADLPPGVGFEPKDAPLRRDINLLGRVLGRVLIEQEGQ
                     DLFETEEEIRTLCKRLRFDYDPELDARLRRRIDAMSGEELRRIVRAFSVYFQLVNIAE
                     RYHRIRRRRQYEASPENPPQRASVASALARLKREGLDRDTLRGLLDGMEIGLVLTAHP
                     TEATRRSVRRKHARIGEMLEAMESPVLTWRERRSLEEDMAEEITLLWQTDELRVRRPE
                     VRQEVERTLLFFEDPLISATLEAYREFEDELLRQYPEDTPSLGRVLEFGSWVGGDQDG
                     NPFVGPEMITASLGLHREVILKRHLASVLLLADHMSQSTRMIPVSTELRRSVERDEEM
                     MPEIAESFGGVDPNEVYRRKLLFMAERLRSTLDQQASSPAYADAAEFLGDLSTVRDSL
                     LENGGQRVADRMLRDVIRQAEVFGFHLATLDVRQESSTVVKAVSELISAATGDDLLAM
                     NEAERAGLLRRLLTDPQLHLPEPNSASEESRKVFETFERIRLAREEFSGSPVETFVLS
                     MAHHASDVLSVQLLARRAGLLEVDGNGRCSANHLRVSPLFETVDDLERAPEVLRYLLE
                     DPFYRSSLGEAGDLQEIMLGYSDSGKDAGYVTSNWTLYQAQGLLSAVAARFGVELRLF
                     HGRGGSAGRGGGPSYQAIMAQPPETLQGRIRITEQGEVISFKYSMRGLARRNLDTVLA
                     AVLEASANEAPREPEGPWIGAMEELSETARETYQALVYEDNDFLTFFSQASPIGELSL
                     LNMGSRPARRVQNPTVDSLRAIPWVFAWTQNRFLLPSWYGAGTAFSRYTADQEKLGLL
                     RRMYGGWPFFRTLVDFMQMTLAKSDLRVAENYTSLVSDSGVRERMWSRISEEHAACVD
                     ALLLITGNNSLLDDSPVLQRSVRLRNPYVDPLSYVQVTLLRRLRAYEEDAPERGVTLN
                     TLLLSISGVSSGMLNTG"
     gene            complement(5595..6725)
                     /locus_tag="AC16CM_08740"
     CDS             complement(5595..6725)
                     /locus_tag="AC16CM_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3139959.1"
                     /translation="MVATQNKTSRNESAFSEIKRLSAANLEGPELLRRVARRLSKAVP
                     FEAYCASTVDPTSRLITFGIAEGMGDEGDAEGGNVYLNRIYFEEDLPVMNDMLRRRRT
                     VQLLSETTQGELERSLRYRELLKPSGFGYELGGAFVDGNLWGGMDLIREEGDPDFDGA
                     EAALVKRIAPHVGAGLKAAALRSRATREQDTPDVPGVLTLDRSGIVVSHTPSAEHWLR
                     DLEDLRPGWRRENIPMVVRMVSGALRHALSPGSDSDHNLVPRVRVRGRSGRWLALYGS
                     LTESSEERPGETVIVIGPAKPEEVAWLTLAAYGLSPREEEVVDLVVRGFANRQISQTL
                     FISEHTVQRHLSNIFEKVGVRSRKNLVKRLYFENLLPNMAGD"
BASE COUNT         1296 a         2199 c         2194 g         1359 t
ORIGIN      
        1 gtgaagatcc tggcctgtac ctcgtctctg aagtcctcgt cacgcaacat caggggctcc
       61 gacaggacgt tgctacgaag gccctcctta agaagcgggg ccgtcaggtc cggccgcgtg
      121 aggatcaggg tgagcaccgc gcctcctgct tcagtgtggg ggtccgacct acggcggcga
      181 ctcggcgcct gcgactggtc ggttgtggtc tccttctcta tcagcacgtc gtggctgacg
      241 ccgagtgact cggtggcaag ccgcagcgcc tcgcgataca ggacggggtc tttgatctcc
      301 ccgatcatgc tccttatctc tgggacggcc ctggagcgct ctgtggcccc ggccccacgc
      361 gtgcgggcta cgatgcgccg gatgccgtac tcgagaacgg gtaaagcgcc ctctagcagc
      421 tgcgcaaact cttgtggcgg gtgttgaagc aaccagtcgg ccgggtcctc ggggaggcgc
      481 aggacgcgta ggtccaactt caactccgcg gccgtcgcgg cggccctctc tatcgccctc
      541 tcccccgcca catcgggatc aaagagcacg tagatcctgt ccgcgtagcc gctcagcatt
      601 cgcaggtgcc cggcggtggt ggccgtaccg agggtggcca cggcgttctt tatgcccgac
      661 tggtagagca tgagcacgtc ggtgtagccc tccaccacga gcgcggcccg ctccttgcgc
      721 atggcctctg agacctgagg aaagccgtac agcagatccc gcttgttgaa gacatccgtc
      781 tcaggcgagt tgaggtactt gggctgggca tcacccagcg tcctcgcccc gaaaccaacg
      841 atcctgcccc gccggtcgga gatcgggaac gttatcctgc cggcgaaacg ctcccctccc
      901 cgccccgata aaagaccggc cgcctcaagc gtgctcctgt caaggttcaa cctctttgcc
      961 gtccccagaa aacccgcaac tccccgcggc ggagcgtacc ccaaacgaaa ttcttctata
     1021 gtagaatgct cgattttgcg gtcccgaaga tagtggcggg cctgctcggc ttccggagtc
     1081 cgggagccga gcaggtactt gtggtagtag acggtagcgg cggcgagggc cctgtggatc
     1141 tcacgccgcc gctgggcgcg ttgcctcgcc gcgtcagggt ctcccccacc ctcgaactgg
     1201 agctcgattc cggatcgttc ggcgatatag gagaccgctt cggcgaagtc caaggacttc
     1261 aggtccatga ccagcttgat ggtgtcgccg ccccgctgac aattgtgagt tatacccatt
     1321 ttggtgagga acgtgtgcga acccgtggtg gttatatcca ctaccgtagt gggttcttcg
     1381 gtctcgggct cgacggcctg gactatcgac cagaaatacc tggtgccgtc aatctcggcg
     1441 aaaaagccct tcagagattc tttgctgagg gcgtgaacgt aatacgttct cctgcgccat
     1501 ataccgtccg ccttcctccg ttcgggcatg gtggaggtag agcagaccct ccccatctgg
     1561 atgcagaggg cgaagatacc ataggtcagc gcctctgaga ccgaagccgc tgacgtcacc
     1621 ccatcgctcg tgtgactttc tccatcgaaa taaccctgga caagagcggc ctgacactcc
     1681 gtggaccagg acaatgctct tacaggaagc cttttgctcg cacacccccg accgaaccag
     1741 tactggaaca aagttgacag gtccgtgctc gagcacgtga cctcacacag gttcttgtgt
     1801 ggccgccgaa agcgggtgct gggtctgccg aattccgtct tcaacgtttc tactaccctt
     1861 gcggctagag tatcggcctc gctaatcgaa aagctccact tcacgccgcc tcggtagagc
     1921 gatcccttgg cgagccagac cccgtacagc caggccgtct cagggtttac aggtagttcc
     1981 gcgatgcgcg cattgcgtgg tcctctggtg tactccttta tgacttgctg accctgtagg
     2041 gctgaatcat ctctttcctc gtccgagatg acggggtaga gccagaaatc tccttctcga
     2101 acctccgaag catgatccac ggataccatg acgtgtgcgt ttttcgggtt gtgcctgctg
     2161 gagaagcgga gcttctttcc gttggactgc ctgggatgga ccctgggaat agcacgtagc
     2221 gcttcttcct tccgcacgaa gacgcagtga tggtctgggg tgagctgtag accttccttg
     2281 gctgctccgg tcttgattct caaggtcggg ccggatttga accacttgtc cgtaatcgtc
     2341 tcgcgggagc cgtcgagccc gatcacctca tcacccggtt cggcttcggc gatcgggatc
     2401 agacccctcg aggtccagat ccgctcgttc gcctcgaggc aaccaaaaca gtaatagaaa
     2461 ccgcggtcgg gggagacgct gaaggagggg gttttctcct ggtggtcggg gtaggggcag
     2521 aggcccgaga agcgggctcc ctgacgtttc agggcggtga actctgaggt cacctcgacg
     2581 atatcagcgg cctccctgac ctcctctatg cttcgttgtg cgatcctcaa gtaaagcctt
     2641 ccgttgtacc gatgtacaaa tattaccggt ccggcgcgat gtacggcagg cgtaatgtta
     2701 ctctaccggg gtgaacatcc gcgatgactc atccatggtc ccacgtgcgg ggtatgcgga
     2761 tctcccgccg ggggtaggtt tcgagccgaa ggatgctcct ctgcgcaggg atatcaacct
     2821 gctcggaagg gtactcggcc gcgtcctcat cgaacaggaa gggcaggatc tcttcgagac
     2881 cgaggaggag atccggacgc tgtgcaagcg cctgcgtttt gattacgacc ccgagctgga
     2941 cgcccggctc aggcggcgga tagacgccat gagcggtgag gagttgaggc ggatagtgcg
     3001 cgccttctcg gtgtacttcc agctggtcaa catcgccgag cgctaccacc ggatacggcg
     3061 caggcggcag tatgaggcat ctcccgagaa cccaccccag agggcttccg tcgcgagcgc
     3121 gctcgcgcgc ctcaagcggg agggtctcga ccgggacacg ctgcgcggtc tgctcgatgg
     3181 tatggagatc ggcctcgtct tgaccgccca tccgacagag gccaccaggc ggagcgtcag
     3241 gcgcaagcac gccaggatcg gggagatgct cgaggccatg gagtcgcccg tgctcacctg
     3301 gagggagcgc cgaagcctgg aggaggacat ggcagaggag atcacgctcc tctggcagac
     3361 ggatgagcta cgggtccgcc gtcccgaggt aaggcaggag gtggagcgga cgctgctctt
     3421 cttcgaggac cccctcatct cggccacgct cgaggcctac agagagttcg aggacgagct
     3481 attgcgccag tacccggagg ataccccgtc gctcggacgc gtcctcgagt tcggctcctg
     3541 ggttggtggg gaccaggacg gcaacccgtt cgtcgggccc gagatgatca cggcctccct
     3601 gggcctacat cgagaggtga tactcaaacg tcatctcgcc tccgtgctct tgttggcaga
     3661 ccacatgagc cagtcgacca ggatgatccc ggtctccacg gagctccgtc ggtcggtcga
     3721 acgggatgag gagatgatgc cggagatcgc cgagagcttc ggcggcgtcg acccgaacga
     3781 ggtctaccgg cgaaagctgc tcttcatggc cgaacgcctc aggagcaccc ttgaccagca
     3841 ggcctcatcg ccggcttacg ccgacgcggc cgagtttctg ggggacctct cgaccgttcg
     3901 cgacagcctg ctcgagaacg gcgggcagcg tgtcgccgac aggatgttac gcgacgtgat
     3961 acgacaggcc gaggtcttcg gcttccacct ggctaccctc gacgtccgcc aggagagctc
     4021 taccgtggtc aaggccgtgt cggagctgat ctctgcggcg accggagatg atctcctggc
     4081 gatgaacgag gcagagcggg ccgggcttct gcggcggcta ctcacagatc cccaactcca
     4141 cttacccgag ccgaatagtg cctctgaaga atcgcgcaaa gtattcgaaa ctttcgagcg
     4201 catccggctg gcgagagaag agttctctgg gtcgccggtc gagacgttcg tgctgagcat
     4261 ggcccaccat gcgagcgacg ttctaagcgt gcagttgctc gcgcggaggg ccgggcttct
     4321 agaggtagat ggcaatggcc ggtgcagcgc caaccacctc agggtctcgc ccctcttcga
     4381 gaccgtggac gacctcgagc gggcaccgga ggtgctcagg tatttgctcg aggacccgtt
     4441 ctacaggtca tcgctcgggg aggccggcga cctgcaggag atcatgctcg gatacagcga
     4501 ctcgggcaag gacgccggat acgtcacgag caactggacg ctttaccagg cgcagggatt
     4561 gctctctgct gtggccgccc ggttcggggt cgaactacgg ctcttccacg ggcggggcgg
     4621 cagcgccggc cgcggaggcg ggccgagcta ccaggcgatc atggcccaac ccccggagac
     4681 gctgcagggc aggatccgta tcaccgagca aggagaggtg atctccttca agtacagcat
     4741 gcgcggcctt gcccgtcgca acctggatac ggtgcttgcc gcggtcctcg aggcgagcgc
     4801 gaacgaggct ccccgcgaac cggaggggcc ctggatcggg gcgatggagg agctatccga
     4861 gacggcccgc gaaacctacc aggcactggt ctacgaggac aacgatttcc tgaccttctt
     4921 ctcccaggcc tcacccatag gagagctctc gttgctcaac atgggcagca ggccggcccg
     4981 ccgggtgcag aacccgacgg tcgatagcct gagggctata ccctgggtgt ttgcctggac
     5041 ccagaaccgc ttcctgctac cgtcctggta cggcgccgga acggccttct cgcgctacac
     5101 cgccgaccag gagaagctag ggctactgcg ccggatgtac ggggggtggc cgttcttccg
     5161 aaccctcgta gatttcatgc agatgacgct ggccaagagc gaccttcgcg tggccgagaa
     5221 ctacacttcg ctggtatccg actccggcgt gcgggagcgt atgtggtcaa gaatctccga
     5281 agaacacgcc gcgtgcgtgg atgctctgct cctcataacg ggcaacaaca gcctgcttga
     5341 cgacagccct gtgctgcaga gatccgtccg tctgcgaaat ccttacgtgg accccctctc
     5401 ttacgttcag gtaaccttgt tacgccgact ccgcgcctac gaagaagatg ctccggagcg
     5461 cggggtcacg ctcaacacgc ttctgctctc tatctcaggg gtatcttcgg ggatgctgaa
     5521 cacaggatga gaggcgcgtc gaccgccggg cttcagggat gcggcctgca ggattctggg
     5581 ccccgtaatc ctctctagtc gccggccata ttcggtagaa ggttttcgaa gtacaggcgc
     5641 ttcacgaggt tcttgcggct cctgacgccg actttctcga atatgttgga gaggtgacgc
     5701 tgcacggtgt gctctgagat gaacagggtc tgtgagatct ggcggttggc gaagcccctg
     5761 accacgaggt ccactacctc ttcttcgcgc gggctgagac cgtaggcggc gagggtaagc
     5821 caggccacct cctcgggttt ggccggcccg ataacgatta ccgtctcgcc gggacgttct
     5881 tcggaggatt cggtgagcga gccgtagagc gcgagccatc gccccgagcg gccccgtacg
     5941 cgcaccctcg gaaccagatt gtggtcgctg tccgatcccg gggagagagc gtgcctgagc
     6001 gcccccgaca ccatcctgac caccattggg atgttctccc ggcgccagcc aggacggaga
     6061 tcctccagat cccggagcca gtgttccgcc gacggggtgt gggagacgac aattccagat
     6121 cggtcgaggg tcagcacgcc aggcacgtca ggggtgtctt gctccctggt ggctcgggag
     6181 cggagggccg cggctttgag gccagccccg acgtgtggcg ctatgcgctt caccagggcc
     6241 gcttcggcac catcgaagtc gggatccccc tcttcgcgta tcaggtccat accgccccac
     6301 aggtttccgt cgacgaaggc gccgcccagc tcgtagccga agccagatgg ctttagtagc
     6361 tccctgtacc gaaggctgcg ctcgagctcg ccttgcgtgg tctcggagag caactgcacc
     6421 gtacgccgtc tgcgaagcat atcgttcatt accggaaggt cttcctcgaa gtagatgcga
     6481 ttcaggtaga cgttaccgcc ctcggcgtct ccttcgtccc ccatcccttc agcgataccg
     6541 aaggtaatca gacggctcgt gggatctacg gtcgaggcgc agtaggcttc gaagggcact
     6601 gccttgctca acctcctggc cacgcggcgt agtagctctg ggccctcgag gtttgccgcc
     6661 gagagacgct ttatctccga gaatgcactc tcgttgcggc tcgtcttgtt ttgtgtggca
     6721 accatttctt gaccagcctc cgaaagcgta ctcgccaacc tcgctctgtt gctcacatga
     6781 tagcccgcgg gctgggaagg gcacatcacg caaaatgact agttgcgcaa actttcgagc
     6841 gggcaaggaa atggcaggtt tctgctatgt gccccgcacg accacgggtc tattctcgtt
     6901 accgtcgaga cgtacaacgg cgaaagagag cgaggaagcc aaatgcccac cgacaagaac
     6961 gcgaatacga tccgccgcat ctacgacgag ctgtggaacg agcggaacct ggcgctcgcc
     7021 gatgacctca tcgccgagga cgccacca
//
LOCUS       DBNGVD010000327         2137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4446944, whole genome shotgun sequence.
ACCESSION   DBNGVD010000327 DBNGVD010000000
VERSION     DBNGVD010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4446944"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16CM_08745"
     CDS             <1..243
                     /locus_tag="AC16CM_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139960.1"
                     /translation="RRVGYVRSILERWVERGVKSVEDVERFEKDRKLRLVEQSGAVKG
                     GALRVRESGGGVSGGQDRQSAARRREGYEWLFGETE"
     gene            215..1033
                     /gene="zapE"
                     /locus_tag="AC16CM_08750"
     CDS             215..1033
                     /gene="zapE"
                     /locus_tag="AC16CM_08750"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HIR3139961.1"
                     /translation="MSGSSGKRSKAEVLRLDDQVCPHCGRELQAEWDEFPPALQKKYG
                     KPGEWYYHPCTPECEKKNERREWELMRRNARVASLKERSGLSKRMKSYALDNFDPTLS
                     KNTQKAFFKVMDYMRGWKDNQEEGRGLYFCGPVGVGKTHLAVAVMNEIITKKRVPALF
                     VTVPELLDNMRGTYNAPGRDIDQWMDSVKNAELLLLDDLGAERANDWVRERIFVIVNH
                     RYREELPTIFTSNIGPRDLAAQLGERTASRIIAMCDWISLEGEDYREAEVRRGL"
     gene            1030..1692
                     /locus_tag="AC16CM_08755"
     CDS             1030..1692
                     /locus_tag="AC16CM_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139962.1"
                     /translation="MRDEFMITRQGKQYVLFAGLLDEAHSLGLKGIDTDLLQIPDESN
                     GNVAIVKATAEMDDERRFSGIGDASPDNVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TAMEELSDGDDAPPASSQPRGTRPTPIRGGKSNSGPPPQDAPAEAEEAPPNGQAQKPA
                     AKRGGKARKSQVDLLKTLAVEWRGENGVERLETRLGKPLTDLTRTEADEWIDRLTPEG
                     RE"
     gene            1696..1941
                     /locus_tag="AC16CM_08760"
     CDS             1696..1941
                     /locus_tag="AC16CM_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139963.1"
                     /translation="MRSTVGLVLGALGVVCALIGLALINGISIEFPGIILGALGYYFC
                     LPNGDRVSSRPGQILGIVAVVLSVVSIVLSGLAGPPQ"
     gene            1938..>2137
                     /locus_tag="AC16CM_08765"
     CDS             1938..>2137
                     /locus_tag="AC16CM_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3139964.1"
                     /translation="MTLSREDQALVEQAKAILALNWTGEYTQPGPRLYPHQWSWDSAF
                     VALAYAHYDHARAEKELRHLFEA"
BASE COUNT          440 a          663 c          734 g          300 t
ORIGIN      
        1 cgcagggtcg gctacgtgag gagcatcctg gagcggtggg tcgagcgggg cgtcaagagc
       61 gtcgaggacg tggagcgctt cgagaaggac cgcaagctca ggctcgtcga gcagagcggg
      121 gccgtcaagg gcggtgcact cagggtaaga gagtccggag ggggggtgag tggtggacag
      181 gatcgacaat ctgctgccag acgcagggag ggctatgagt ggctcttcgg ggaaacggag
      241 taaggccgag gtactgaggc tcgacgatca ggtctgcccc cactgcgggc gcgagctgca
      301 ggctgagtgg gacgagttcc cgccagcgct gcagaagaaa tacggcaaac caggcgagtg
      361 gtactaccac ccctgcacgc ccgagtgcga gaagaagaac gagaggcgcg agtgggagct
      421 catgcgccgc aacgcgcgcg ttgcgagcct caaggagcgc agcggcctgt ccaagcgaat
      481 gaagagctac gcactggaca acttcgatcc cacgctcagc aagaacaccc agaaggcctt
      541 cttcaaggtc atggactaca tgaggggctg gaaggacaat caggaggagg ggcggggcct
      601 gtacttctgc ggtcccgtgg gtgtaggcaa aacgcatctc gcggtcgcgg tgatgaacga
      661 gatcatcacc aagaagcggg tgccggcgct cttcgtcacg gtccccgagc tcctggacaa
      721 catgaggggc acctacaacg cacccggtag ggacatcgac cagtggatgg actccgtaaa
      781 gaacgccgag cttctgctgc tcgacgacct cggggccgag agggccaacg actgggtgcg
      841 cgagcgcatc ttcgtgatcg tcaaccatcg ctacagggag gagttgccca ccatcttcac
      901 ctccaacata ggacccagag acctcgccgc gcagctgggc gagaggacgg cgagcaggat
      961 catcgccatg tgcgattgga tctccctgga gggcgaggac taccgagaag ctgaagtgag
     1021 gcgtggcctg tgagagatga gttcatgatt acccggcagg gaaagcaata cgtcctcttt
     1081 gcagggcttc tagacgaggc ccacagcctg gggctcaagg ggatcgacac ggacctgctc
     1141 cagatccccg acgagtcgaa cgggaacgtg gcaatcgtga aagcaacggc cgagatggac
     1201 gacgagaggc gcttctcggg aatcggcgac gcgagcccgg acaacgtggg ccgcaacatc
     1261 gcgccgcacc tgatccggat ggcagagacg cgggcgaagg cccgcgccct gcgcgacgcc
     1321 gtgaacgttg gcgcgacggc catggaggag ctctccgacg gcgatgacgc cccgccggcc
     1381 tcgtcccagc cgcgcggaac gaggccaacc cccatccggg gcggcaaatc gaactccggc
     1441 ccccccccac aggatgctcc cgcggaggca gaggaagccc cacccaacgg gcaggcgcag
     1501 aagcccgccg ccaagagggg tggcaaggcg cgcaagagcc aggtggacct cctcaagacg
     1561 ctggccgtcg agtggcgggg tgagaacggc gtcgagcggc tcgagacccg cctcggcaag
     1621 ccgctaacag acctcacccg cacggaggcc gacgagtgga tagaccgcct taccccggaa
     1681 ggacgtgagt agcctatgcg ttccacggtc gggctggtac tcggagccct cggcgttgta
     1741 tgcgcgctca tcggcctcgc cctgatcaac ggcatctcca tcgagtttcc cgggatcatc
     1801 ctcggcgctc tggggtacta cttctgcctg ccgaacgggg accgggtcag cagccggccc
     1861 gggcagatcc tgggtatcgt cgcggtggtc ctcagcgtcg tatcaatcgt cctgagcggc
     1921 ctcgcgggac cgccgcagtg accctgtcca gggaagacca ggcgctggtc gagcaggcca
     1981 aggcgatcct ggccctcaac tggaccggcg agtacaccca accgggcccc aggctctacc
     2041 cgcatcagtg gtcctgggat tcggccttcg tcgccctggc ctacgcccac tacgatcacg
     2101 cccgtgccga gaaggaactc aggcacctct tcgaggc
//
LOCUS       DBNGVD010000328         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1351792, whole genome shotgun sequence.
ACCESSION   DBNGVD010000328 DBNGVD010000000
VERSION     DBNGVD010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1351792"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="AC16CM_08770"
     CDS             complement(<1..422)
                     /locus_tag="AC16CM_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139965.1"
                     /translation="MLKVLYSLAIALLVAFFVGFGISAFYQAPQRPPMASAKVDAAQK
                     AENPTQEQKQLLSERRQSWKAYHQQVSDYNKVVSSIAIGVAVVLLGGSILWLSGLAVI
                     GDGVTLGAVFTLFYGLIRANMTDSEVFRFIAVAVGLVVV"
     gene            complement(710..1180)
                     /locus_tag="AC16CM_08775"
     CDS             complement(710..1180)
                     /locus_tag="AC16CM_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139966.1"
                     /translation="MSTDNPDSSTLPRLLSVPGLVSIAVFGVLVVVVARIFGPENILR
                     EVVVELLASFGSTILVLAIFGLIFRSGLQRLLRSAPGGETFSRSAERLGELLQEWDQR
                     EDSEDTHITEKLDRIEEGIKTLSTDEIASLQNEIRELRTMLADRGGGQVDETSG"
     gene            complement(1232..1807)
                     /locus_tag="AC16CM_08780"
     CDS             complement(1232..1807)
                     /locus_tag="AC16CM_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139967.1"
                     /translation="MNRLRLAEIVSGATRLPLLAVPLFLAVGVSAAGRVGLWWALLCI
                     FLTSGFSLLYLVYLTSSGKVSDPRAIPRAERVGPLRVVAVLHVGAFVVVSLLGGPAEL
                     RAALLSYALATLGFAALTPVSNLSLHAAGVSGTAVCLAYVFGAWGFLGFLLLPPVLWA
                     RLALGRHTPFELALGVLVGGAGTWISFQLIT"
     gene            1844..2911
                     /locus_tag="AC16CM_08785"
     CDS             1844..2911
                     /locus_tag="AC16CM_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR3139968.1"
                     /translation="MADVADAVERIAPSSLAEGWDNCGLQAGDPAAVVGRVLVALTPL
                     PEVFAEAEERKADFLLFHHPLIFQPIRAFDTSLYPGNLLARALRRDAAVYAAHTSYDA
                     APGGVSVALAEAIGLQGPLRVVSPRGTLRKLVVFVPEESVDSVVLALSEAGAGVIGDY
                     TECTFRTRGTGTFRGGDETNPYLGESGRLESVEEVRVETVVAGHLAGRAAKAATAAHP
                     YEEVALDVYTVEGTPEGCGYGRLGTLPGPLSPEELRQHVSGSLGFPARLVADAGGYIQ
                     RVAVLGGSGGSFIPEVAAAGAEAYVTGDVDYHDALLAETLDLTVIDAGHAATELPSLA
                     PLARRVGELVDVPVEISRVRR"
BASE COUNT          581 a          954 c         1017 g          538 t
ORIGIN      
        1 accaccacta gccccacggc cactgcgatg aacctgaaca cctcactgtc ggtcatgttc
       61 gcccgaatca acccgtagaa cagcgtgaag accgccccga gcgtcacccc atcgcctatc
      121 acggccaggc cgctaagcca gaggatgctg cccccgagta gtaccaccgc cacgcctata
      181 gcgatggagg agaccacctt gttatagtca gatacctgct gatgataagc tttccagctt
      241 tgccttcgtt cgctcagcaa ctgcttctgt tcctgggtgg gattttcggc cttctgggca
      301 gcgtccactt ttgcagaagc catggggggc cgctggggtg cctggtagaa ggcggagatc
      361 ccaaacccga cgaagaaggc caccaggagg gcaatagcca gggaatagag taccttgagc
      421 attttcgatc aacctcccta gccagggatc tcttcactag tcagtgtgct gcaagggggc
      481 cgttctgtca tgatacatac agagcatttc attgatcaag attgatcaag aaccgtaagt
      541 atggcaagcg aaggtaccca tcatccccat ggcctccgcc cgccctgtgc cgcgcccctc
      601 ctacgaccga cccgaggaca gctgggagat cggtcggtgt gaagaagctt ctacgcccgg
      661 ccaggcgcga gattttccta cgccagagat cgtagcgttg gctctcgcct cacccggagg
      721 tttcgtcaac ctgcccacca ccgcggtccg cgagcatggt gcgcagctct ctgatctcgt
      781 tctgcaagct tgctatctca tccgtggaca aggtcttgat gccttcttct atccggtcca
      841 gtttctcggt gatatgggta tcttctgagt cctccctctg atcccactcc tgcaggagct
      901 cgccgaggcg ttctgcggag cgcgagaagg tttctccgcc gggggcgcta cgaaggaggc
      961 gttggaggcc ggatctgaag atcaagccga agatggcgag cacgaggatg gtgcttccga
     1021 agctggcgag tagctccacc acgacctccc tcaggatgtt ctcggggccg aagatgcgcg
     1081 ccacgacaac gaccaggacc ccgaacacgg cgatgctcac gaggccgggc acagagagta
     1141 gcctgggcag cgtcgaggaa tcgggattgt ctgtgctcat cgtgtctcct tgctgatatc
     1201 tcgggaattc tggcgggcca ttatcaacgc ctcaggtgat tagctggaat gagatccagg
     1261 tccccgcgcc cccgacgaga acccccaggg caagctcgaa tggcgtgtgg cggcccagag
     1321 caagcctggc ccatagtacg ggcgggagga gcaagaaccc tagaaatccc cacgctccaa
     1381 acacgtaagc caggcagacg gcggttcccg agacgcccgc ggcgtggagg gagaggttgc
     1441 tcactggcgt tagcgcagcg aagccgagcg tggcaagcgc gtaggagaga agcgcggccc
     1501 tgagctccgc cggcccgccg agcagcgaca cgacgacgaa ggccccgacg tgcagcaccg
     1561 cgaccacccg caagggcccg acccgctcgg cgcgcgggat ggcacgcggg tcgctgacct
     1621 tgcccgaact ggtgaggtag acgaggtaga ggagcgagaa cccgctggta aggaagatgc
     1681 agagaagtgc ccaccataac cccaccctac ccgcggcact tacccctacc gccaggaaca
     1741 acgggacggc aagcagcgga agacgggttg cccccgagac gatttcggca agcctgaggc
     1801 ggttcacgga ggaggattgt acaatgcccg gaccttggta cgcgtggcag acgttgcgga
     1861 tgcggtggag cggatagccc cgtcgtccct ggccgaaggg tgggacaact gcggcctgca
     1921 agccggcgac ccggcggctg tggtggggcg ggtcctggta gcgttgacgc cgctgcccga
     1981 ggtcttcgct gaggccgagg agaggaaagc ggactttctc ctgtttcatc acccccttat
     2041 cttccagccg atacgggctt tcgacacctc actctatccc gggaatctcc tcgccagggc
     2101 cttacggaga gatgctgcgg tctacgcggc gcatacgagc tacgacgccg cccccggggg
     2161 tgtgtcggtc gccctggcgg aggccatcgg cctgcagggt ccgctgcggg tcgtctcgcc
     2221 gaggggtaca cttcgcaagc tggtcgtctt cgtgcccgag gagagcgtgg atagcgtggt
     2281 gctggcgctc tctgaggccg gtgccggggt gatcggtgac tatacagagt gcacgttcag
     2341 gaccaggggc acgggcactt ttcggggcgg tgacgagacg aacccctacc tcggggagag
     2401 cggccggctg gagagcgtcg aagaggtgcg cgtcgagacc gtggtcgcgg gccacctagc
     2461 gggccgcgcg gcgaaggccg ccaccgcggc ccatccctac gaggaggtcg ccctggacgt
     2521 atataccgtg gaaggaacac cagaaggctg cgggtacggg aggttgggga ccctgcccgg
     2581 tcccctctcc cccgaagagt tgcgccagca cgtctcgggc tcgttggggt tcccggctcg
     2641 gctcgtagcg gacgcgggag gatatatcca gcgggtggcg gtgctcgggg gatccggcgg
     2701 ttcgttcata ccggaggttg cggccgcggg ggccgaagcc tacgtcacgg gggacgtcga
     2761 ttaccacgac gcgctcctcg ctgagacgct ggacctgacc gtgatcgacg cgggacacgc
     2821 cgccacggag ttgccctcgc tcgcgccgtt ggcgcgcagg gtgggtgagt tggtggacgt
     2881 gcccgtggag atcagccgcg ttcgccggta gccggtaggt cgtcaggcgg ttcacgaacc
     2941 gcccccacat gggggttggg ggatcgggcc gtggcgtttt tcgaacgcgc gtacccgccc
     3001 cccgatcgtt cttcgtctct tcgattaagc gtagccggac gataaactat cgcccagaac
     3061 aagaaagctg atagctgaag gctgatagct
//
LOCUS       DBNGVD010000329         2957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2807678, whole genome shotgun sequence.
ACCESSION   DBNGVD010000329 DBNGVD010000000
VERSION     DBNGVD010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2807678"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="AC16CM_08790"
     CDS             complement(<1..248)
                     /locus_tag="AC16CM_08790"
                     /inference="COORDINATES: protein motif:HMM:NF046346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139969.1"
                     /translation="MGGRHPGPDRHRETARVGCRFVQEFSTSETYRWGVVGITERKDV
                     MDYRLELVPLPVADVDRAKAFYSEKVGFTEDLDHSGEGG"
     gene            complement(197..877)
                     /locus_tag="AC16CM_08795"
     CDS             complement(197..877)
                     /locus_tag="AC16CM_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3139970.1"
                     /translation="MSIVSEERRSDSPYVEAVTRGRTESDGSTVRPAESRWHLVVVRH
                     AGAARALAVGPLTGSGVASWEAGAEILWIRFSLGTFMPHLPTRTLLDTETVLPDASSR
                     SFWLGGSAWPFPDHENADAFVERLVREGVLARDPVLCAASAGLSPQGLSPRTLRHRFL
                     RAAGLAQGAISQIDRANRAAACLRRGVSIPDVVHEAGYFDQAHMTRSLKRWVGGTPAR
                     IATARRPE"
     gene            complement(948..1238)
                     /locus_tag="AC16CM_08800"
     CDS             complement(948..1238)
                     /locus_tag="AC16CM_08800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3139971.1"
                     /translation="MLTVVARYVVSEGHERTVARLLGQNAEASRAEPGCLEFSVYRDI
                     DDPRAILLYERYTDEDAFQAHRLTPHFHDIIERQVVPLLDERAWTRLEPMPT"
     gene            complement(1388..1996)
                     /locus_tag="AC16CM_08805"
     CDS             complement(1388..1996)
                     /locus_tag="AC16CM_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HIR3139972.1"
                     /translation="MRLSKAAHTTRPWRIHELTRDFRIEDVWALPTPGGPDDFPRLVR
                     QMASGGPRLDSSGPARAVWALRWKVGKLLGWDSPEAGLGHRVPTLRDRLPADLRGVPS
                     GPEFDALPFTSLYQLHDEWAAEIANRTVHGVMHLGWVPDGAGGYRGQMAVLVRPNGLP
                     GAVYMAAIKPFRYLIVYPALMREIGRGWRKSADVDAARSGDA"
     gene            2149..2409
                     /locus_tag="AC16CM_08810"
     CDS             2149..2409
                     /locus_tag="AC16CM_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139973.1"
                     /translation="MIRKINPRAWAAVTMITALLFATSGMALAEAPRTVPNSGASHNC
                     VAATSGVLYFRENGVRLGQDVRKFAPHGGQRDYVQGALQKSC"
     gene            complement(2579..>2957)
                     /locus_tag="AC16CM_08815"
     CDS             complement(2579..>2957)
                     /locus_tag="AC16CM_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HIR3139974.1"
                     /translation="VVEMARGENFAALTTILPDGHPQTQVMWVDADDENLLINTEVHR
                     QKFKNVERDQRVTLTIWDRDDPYSFVEVRGEVVGVEKGQEAREHIDELSHKYHGKPYG
                     PDIQSERVIVKISPLRQVVHTTS"
BASE COUNT          507 a          976 c          957 g          517 t
ORIGIN      
        1 cctccctcgc cgctatggtc gaggtcttcg gtgaaaccga ccttctcgct gtagaaggcc
       61 ttggcgcggt ccacatccgc caccgggagc ggcaccaact cgagcctgta gtccatgacg
      121 tccttccttt cggttatgcc tacgactccc caacggtacg tctcggaagt agagaactct
      181 tgtacgaaac ggcaacctac tcgggccgtc tcgcggtggc gatccgggcc ggggtgcctc
      241 ccacccatct cttgagggat ctggtcatgt gcgcctggtc gaagtagcct gcttcgtgga
      301 cgacgtcggg tatggaaacg ccccgccgca ggcacgccgc cgcccggttg gcgcggtcga
      361 tctggctgat agcaccctgc gccagtcccg ccgccctcaa gaaacggtgc cgcagtgtcc
      421 gtggcgacag cccttgcgga gaaagcccgg ccgacgcggc gcacaagacc ggatctcggg
      481 ccagcacgcc ctcgcgtacc agccgctcca cgaacgcgtc cgcgttctcg tggtcgggaa
      541 acggccacgc ggagcctccc aaccagaagg acctgctcga agcatcaggg aggacagtct
      601 ccgtatccag gagggtcctc gtgggcagat ggggcatgaa cgtgcccagg gagaacctga
      661 tccagagtat ctcggcgcca gcctcccagg aggcgacgcc ggatccggtc aacggcccga
      721 cggccagcgc ccgggcggcg ccggcgtgcc tcacgacgac caggtgccag cggctctcgg
      781 cggggcgtac ggtggaaccg tcgctctcgg tccggccgcg cgtgaccgcc tcgacgtagg
      841 gagaatccga ccgtcgctcc tcggacacga tgctcatacc tctgctgatt gtacgggcgg
      901 ccggcgcgat cccaatcctt catccaaccg gcggtcgacg atccgggtca ggtaggcatc
      961 ggttccaggc gggtccaggc tcgctcgtcc aggagcggca cgacctgccg ttcgatgatg
     1021 tcatgaaagt ggggcgtcag ccggtgggcc tggaaggcgt cctcgtccgt gtagcgttcg
     1081 tagagcaaga tggcccgggg atcgtcgatg tcccggtaca ccgagaactc gaggcacccc
     1141 ggctccgccc ggctggcctc ggcgttctgt ccgagcagcc gtgccaccgt ccgctcgtgt
     1201 ccctcgctca ccacataccg tgccacgacg gtcagcatag tcacctcttc ccctaccccg
     1261 aggccttccc ggacgaaaca ccatagggtc tgtatccggg agcgcgaacc cgagacgggc
     1321 aaggggcttc aggcagcaac ttgctacctg aagcctcttt cggggaggtc gggcatcggt
     1381 ggtgaggtca ggcgtcgccg gatcttgcag cgtccacgtc ggcagatttc cgccatccac
     1441 gtccgatctc tcgcatgagc gccgggtaca cgatcaggta cctgaaaggc tttatcgcgg
     1501 ccatgtagac agccccgggc aggccgttgg gccttaccag gacggccatc tggccgcggt
     1561 agccgccggc cccgtcggga acccagccga ggtgcatgac cccgtgaacg gtacggttgg
     1621 caatctccgc ggcccactcg tcgtggagct ggtacagcga tgtgaacggg agcgcatcga
     1681 attcaggacc ggatgggacc ccgcgcaggt cggctggcaa ccggtcgcgg agcgtcggca
     1741 ccctgtggcc gagaccggcc tccggtgagt cccatccaag cagctttccg accttccacc
     1801 ggagcgccca gaccgcgcgg gcagggcctg aggagtctag ccgcggccca cccgaggcca
     1861 tctgacgcac cagccgaggg aagtcgtcgg gtccgcccgg cgttggcaac gcccacacgt
     1921 cctcgatccg gaagtcccgg gtgagctcat gtatgcgcca gggacgggtc gtgtgcgcag
     1981 ctttcgagag cctcattcgt cgacctccgt gttcgtcggc ctcctggata gtacgcgcct
     2041 ggccccgaga cgcagagcgc atgcccgggc gtgacagcgt atatgatgag gatctgccag
     2101 gcggcattca agctgtggga ccaggatccg gagacggtga ggaggggcat gatcaggaag
     2161 attaacccga gagcgtgggc cgctgtgacg atgatcacgg ctctcctgtt tgccacctct
     2221 ggcatggccc tggccgaggc accaagaacc gtgccgaaca gcggcgcgag ccacaactgc
     2281 gtggccgcca catcgggcgt actgtacttc agggagaacg gcgtgcgtct gggccaggac
     2341 gtgaggaagt tcgccccgca cggcggccag cgggattacg tccagggtgc gctgcagaag
     2401 tcctgctgag caaagagtgc ggtaggtacg tctttcagca ctcagccagg atcggaagac
     2461 gggagagggc aagggaaagg tccggctcca gacccttgat tcccgttgca tgacgtcgtg
     2521 gggaacccct ggtgtagcct gctcggttgc gcaagctagc cttcctcacc ccgctggatc
     2581 aggatgtggt gtggaccacc tgccgcaggg gggagatctt gactatcacc cgctcactct
     2641 ggatatcggg accgtagggc ttgccgtggt acttgtggga gagttcgtct atatgctccc
     2701 tggcctcctg gcccttctcg acacctacga cctcaccacg tacctcgacg aagctgtagg
     2761 ggtcatccct gtcccagatc gttagcgtca cccgctggtc gcgctccacg ttcttgaact
     2821 tctggcggtg cacctcggtg ttgatcaaga ggttttcatc gtcggcatcg acccacatca
     2881 cctgggtctg ggggtgaccg tctggcaata tcgtcgtgag cgccgcaaag ttctccccgc
     2941 gggccatctc cacgact
//
LOCUS       DBNGVD010000330        15007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4995149, whole genome shotgun sequence.
ACCESSION   DBNGVD010000330 DBNGVD010000000
VERSION     DBNGVD010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4995149"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1226
                     /locus_tag="AC16CM_08820"
     CDS             99..1226
                     /locus_tag="AC16CM_08820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3139975.1"
                     /translation="MQDKKTTEHPPGAREAVTGREVAVELAGITKRFGQFVAVDDLTL
                     DIYEGEFFSLLGPSGCGKTTTLRMIAGFEEPTEGGISVSGDPVRGVPPYRRPVNTVFQ
                     SYAIFPHLDVFDNVAFGLRRAGVKGEELQSRVTDACEMVQLSGFEKRKPSMLSGGQQQ
                     RVALARALVNRPRVLLLDEPLGALDLKLRKEMQLYLKDLQHEVGITFIYVTHDQEEAL
                     TMSDRIAVMSEGKVQQVADPPTLYERPNNRFVADFIGQTNVFSGTVEAVDGGRVTLSV
                     PSGEKVEASTGAAEVGVGQATHAVVRPEKVRFGSEGDNVMTVRIHQIVYLGVSIQYIA
                     DLPGGEKLILYQQNTTEEDHPKVGDEAPVSWDARNCLILGG"
     gene            1229..2338
                     /locus_tag="AC16CM_08825"
     CDS             1229..2338
                     /locus_tag="AC16CM_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3139976.1"
                     /translation="MAFRRRISRGDFLRTMGAGVVAGSTLSVLACQPNTTAQNSGGGG
                     SGPEEKELNLYNWSDYVAKSTLPDFQKQTGIKVTQDFFSSNEELLAKLQAGGTGYDVI
                     VPSDYMVSVMIKSDILEQLDMSKIPNFKNVGANYKGLGYDPDNQYSVPYQWGTTGILY
                     NKKRLGTLEPSWDPMWDPDNKGKIGMLNDERETPGAALYKLGYSVNATEKEQLEEAEA
                     ELIKQKPLLRGYFDSTQNRPSVINGDLLLGHVFSGDAFLALSSNEDLDYLIPEPAATR
                     WTDNMAIPKGAEHPDNAHKFINYILGAKTGATLSNYTYYNTPNEAALPMIDDALKNLP
                     GYELEPSVFDRLQIIEDVGAATREYSTLFTSVKSS"
     gene            2342..3208
                     /locus_tag="AC16CM_08830"
     CDS             2342..3208
                     /locus_tag="AC16CM_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139977.1"
                     /translation="MGKQGRGWTVGLAGLLSPVTLWLGLFFLVPLLLILAYSFGKSGI
                     YGGITLGFYPGNYLKVFDPLYLEIIVRTFVIALLNTFLCLLVGYPLAYFITFKGGRHK
                     HTLILLVMIPFWTSLLLRAYAWVVILGGDNGIANRFLQFLHLTDHPLTLIFTPQAVIM
                     GMVYSYLPFMILPLYAALEKFDWRLKEAAQDLGASRWHTFWRVTFPLSLPGVIAGSIL
                     VFIPSAGEFVIPNLLGGARTVMVGNLIQQQFLQARDWAFGSALAVMLAVLLVGAIMFY
                     VRRVGADKLEEV"
     gene            3212..4036
                     /locus_tag="AC16CM_08835"
     CDS             3212..4036
                     /locus_tag="AC16CM_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3139978.1"
                     /translation="MGGAKGGPVYRGVQKVPDRVLLVFAGLVYLFLYAPIMVLIIFSF
                     NSTKNTQVWTGFSTQWYGALIRDGSVLNAFKLSLTVGLTATAIATVIGTFTALALSRY
                     EFRGKTFADSAIYAATVMPEIVVGVSLLVFFVAATIPLGIVTIVIAHVAFCISFVTIV
                     VRARLSGMDRSVEEAAQDLGANPVQTFLRVTLPLILPGVMAGALLAFTLSFDDFVITF
                     FVAGVGSSTLPLKIYSMIKFGVSPVINALSTVVMVSTMILILGGGRILARRQGNAS"
     gene            4033..4470
                     /locus_tag="AC16CM_08840"
     CDS             4033..4470
                     /locus_tag="AC16CM_08840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3139979.1"
                     /translation="MTPAIRPLEAKDFDAWLPLWYGYNSFYRNEVAAEVTENTFRRLH
                     ENTDGFFGLVAEHDGGLVGLAHAIFHPSTWTMKGYCYLEDLFVARSHRGSGVARALIE
                     GVYTEADRRGADSVYWHTQSYNAPARSLYDQVAQSTSFVVYER"
     gene            4555..6108
                     /locus_tag="AC16CM_08845"
     CDS             4555..6108
                     /locus_tag="AC16CM_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3139980.1"
                     /translation="MHSVGGSRNAGSPEVSEHGLKENAIGFRDALIIGIASTAPAYSL
                     AAVIGLVVVVVGVQAPAVLLASFIPMFFVATAFYYMNRADQDCGTTFSWVTRAMGPYL
                     GWIGGWAICVTGILVVGSLADVAARYTYIFFGLDGLAGSKVAITLFAVVIIAATTALC
                     VIGTELSARIQDVMIIAQVGALLLFAVVALVRVYGGTAPEGSLVPKLSWFSPFAVDTP
                     SALIAGLLIGVFIYWGWESSVNLTEETENSESAPGLAAVASTVILLVTYLSVAAAVVG
                     FGGVGTAERFADDDAILSTLATGVLGSPWDKLVVLAVLTSALASTQTTILPASRTVLS
                     MARANALPSALGATHPRFLTPHVSTIVVGALATLWYVPLNFLSENFLFDTLSALSLMI
                     AFYYALTGFACAIYYRHELFKSAKNFLFIGLAPVVGALVLAYLFFRSIIDLASPDASY
                     TGGSFLGLGLPLVIGLGFLLLGVILEIIWRLGGHGSFFGRRPETVSPEIAAGSARVKE
                     AAGAPPEED"
     gene            6112..6540
                     /locus_tag="AC16CM_08850"
     CDS             6112..6540
                     /locus_tag="AC16CM_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3139981.1"
                     /translation="MPGCLILGYDDTPSARAALDVAIEQAKRFDERLVILFAYAPPGR
                     SVGEEYSEHRRVLEEFGSVATSKAADKATAEGVDVEVLLVAERPSIALENAARARGAR
                     LIVVGTYGESPLKSAILGATPHKLLQFSETPVLCVPAGPR"
     gene            6740..8332
                     /locus_tag="AC16CM_08855"
     CDS             6740..8332
                     /locus_tag="AC16CM_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3139982.1"
                     /translation="MAESMGRGDAPRVDGVERPGGDLRDKGLQHDAVGLLASVVLGVS
                     CVAPAYALTATLGPTVSEVGLQMPAVFLAGFVPMLLVAYAYRELNHVAPDCGTSFTWT
                     VKAFGPHIGWMCGWGFVLATVIVLSNLVGVAVTFFYLLLGQVFGSEAIGTLGDNAVVN
                     VLTTLVFVAGATAISYRGMAATKGIQYVLVGLQMVVLALFIVLAFGGAIGGASDFAIG
                     FSWGWLNPFAIESFAAFAAGLSLSIFIYWGWDTTLTVNEETTGSSSTPGRAALLTILT
                     ILGTYLLVAISAQMFAGIGKQGIGLGNPDTADNVFAVLAGPVLGAFAVFLFLAVVASA
                     ASSLQTTFLPAARTMLAMSFYKAAPERFSHVHPRFRSPSYATLFSGVATAIFYTVMTF
                     LSEDVLIDTIYALGLMISFYYSLTAFACVWYFRGELFTSPRNFLFQGLFPGLGGVMLT
                     LLFFKTAVDTFDPAYGSGGAILGMGTVFVIGVGLLLLGVVLMFAWQRRAPEFFRGETL
                     RHDTAVLTTEEDLLEDRDPEDG"
     gene            8325..10244
                     /locus_tag="AC16CM_08860"
     CDS             8325..10244
                     /locus_tag="AC16CM_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HIR3139983.1"
                     /translation="MVELVRHPLDPLTVEEIRKTTAILRRDREMGTRWRFASIELKEP
                     SKDTVAGYSPGDPISREARVVCWNREDGQAYKAVVSLTEDRVVAWEHRPGEQPNITAD
                     EFHECDVALRSDPRVVQVLADRGITDLDKVLFDVWAYGGSLIADRYRGLRVGWADIWY
                     RDHEDSNPYANPVNGLKLIVDLNRMELLEIEDAFRIEKPDVMGEYLPRYVPTPLREEP
                     RPLEITQPEGTSFVLDGNELRWQKWSLRVGFNYREGLVLHTVGYEDGGRVRPVAHRMS
                     FAEMVVPYRDPSPEHYRRTAFDVGEWGLGYMTTSLELGCDCLGEIRYLDAVLSDAAGE
                     PYTIRNAVCIHEEDDAILWKHVDDVAGAEVRRSRRLVVSFHATVANYEYLVYWRFYQD
                     GNIECEVRATGIMVTTSFEEGEQPPYGALVDERTYAPYHQHFLVARLDLDVDGGENTV
                     YMSESEALPVGPDNPHGLALVQRNVPLRTEEEGKQDYDWQTQRSWKVVNEGARNGLGT
                     PTAYKLVPGGCFPAMLDPSSPVLGRAGVIGHTLWVTPFREDERWPCGEFVNQSEEDTG
                     LTRWTRQNRPIENTDVVLWYVFGLHHITRLEEWPVMPVDTVSFWLKPTGFFDRNPALD
                     VPPSRHCHDPVGDTP"
     gene            10241..11230
                     /gene="speB"
                     /locus_tag="AC16CM_08865"
     CDS             10241..11230
                     /gene="speB"
                     /locus_tag="AC16CM_08865"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3139984.1"
                     /translation="MIDPLQRWASVGEKPDYAGPLSYGGVPYTQDAVSLEGADVAIVG
                     APMDELVSDRPGTRFAPRAIRAASCPPGPQLESGVDALTRLRVVDYGDAPILPADPVR
                     SHAAIKETVGQVLAAGAIPVVLGGDHSITEPGVRACVGAHGPVGLVHFDTHTDTGREV
                     FGAGLSHGTIMRRLVEEGSVDPERYVQIGLRGYWPGEEEFTWQKDRGITSLFMHEVRD
                     LGIEEVTERAIEVVGWGPVFLTVDIDVLDPAFAPGTGTPEPGGMTTAELLLAVREVAR
                     QLDVVGADMVEVIPTGVGSADVTALAADRIVREILGGIAQHRKEKERQQKEEA"
     gene            11227..12663
                     /locus_tag="AC16CM_08870"
     CDS             11227..12663
                     /locus_tag="AC16CM_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HIR3139985.1"
                     /translation="MTETRTLQNFVGGERVEPVDGGTYDLLNPATGEVFSQAPLSGEE
                     DVDRAYRAAERAFETWRETTPSERQLALLRIADAIEERAEEIVRAESENTGKPFGLTM
                     EEEVPAGVDQIRFFAGAARVLEGKSAGEYLEGYTSFIRREPIGVIGQVTPWNYPMMMA
                     IWKFAPAIAAGNTIVIKPSDTTPISTVMMAEIASEFLPEGVFNVVCGDRDTGRALVQH
                     PTPQMVAITGSVRAGMQVAEAASYDLKRVHLELGGKAPIISFDDADIEEAAEAIAGAG
                     YFNAGQDCTAATRVLAAPRIYDDFVDAITEQAKNTRTGDPNDEDTDYGPLNNSNQLAH
                     VSGMVERLPDHAEVTVGGERFGDRGYFYSPTVVSGLEQDDEASQNEIFGPVITVQHFS
                     EEDEAVRWANGVKYGLASSVFTRDHGRAMRVSRRLDFGCVWINTHIPLVAEMPHGGFK
                     HSGYGKDLSMYGLEDYTRIKHVMSNIGA"
     gene            12797..14398
                     /locus_tag="AC16CM_08875"
     CDS             12797..14398
                     /locus_tag="AC16CM_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HIR3139986.1"
                     /translation="MTTCGEALVGLLEAYGIDTVFGIPGVHTVELYRGLPDTRLRHVT
                     PRHEQGAGFMADGYARVCGRPAACFVITGPGLTNIATALGQAYADSVPLLVISSVNNR
                     HELGLGEGRLHELPSQRNLMAGLTAFSHTLQRPDELPAVLARAFAVFDAARPRPVHIE
                     VPLDVITAPADHVQKQRAAFVSRPGPAGEAVAAAAELLRGAERPLVVLGGGASEAAPQ
                     ARALVERLGAPVVTTVNGMGIVPFDHPLCTGSTLSQPVVLEELAQADVVLAVGTELGE
                     TDTLLFDGRPALNGRLIRVDIDPGQLAGNALPDVAILGDAGPALEALERELSGHEADT
                     TAAEARARTLRELSQRRLDGRQRLHQRFVAAVQEALPGVILVGDSTQPIYSANMFYGA
                     GRPRSYFNSSTGYGTLGYALPAALGAGLARPERPVVALLGDGGLQFTVGELATAVELE
                     MPIPILLWNNHGYAEIERYMVERGIPQEGVDIYTPDFLAIALGFGCRAERAEGLEHLG
                     ALLRRSVLEAGPTLIEVDEREALAW"
     gene            14433..>15007
                     /locus_tag="AC16CM_08880"
     CDS             14433..>15007
                     /locus_tag="AC16CM_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3139987.1"
                     /translation="MNLLDYLIMLLYFAVMIGAGYWGLTRARSADDYLVAGRRLGPFM
                     YIGCLSAVVLGGASTIGGVALGYENGISGMWLVFWIGMGVIALGLLMSTRLSRLGVYT
                     VAEMLENRYGAPSRLISALIIAAYALMIAVTSTIAIGTVFKVVLGLSPTVAILVAGGI
                     VVAYSVAGGMWSITLTDIIQFCIMTVGIFFLL"
BASE COUNT         2618 a         4867 c         4875 g         2647 t
ORIGIN      
        1 tcgaatccgg agagcgggtg gctcgggagg tgctctcggg cctcgacggc acaacgcagg
       61 ccacgaccag gcaggttcgg acgtacagga ggtaagggtt gcaggacaag aaaacaaccg
      121 agcatccacc aggggcaaga gaggccgtga cgggcaggga ggtggccgta gagctggccg
      181 ggatcaccaa gcgcttcggg cagttcgtcg ccgtggacga cctcacgcta gacatctacg
      241 agggcgagtt cttctcgctg ctcggcccga gcgggtgcgg caagaccacg acgctgcgca
      301 tgatcgccgg cttcgaagag ccgacggagg gcggcatctc cgtctccgga gacccggtgc
      361 gcggcgtgcc accctaccgc aggccggtca acaccgtgtt ccagtcctac gctatcttcc
      421 cccacctcga tgtcttcgac aacgtggcct tcggcctccg ccgcgccggc gtgaaggggg
      481 aagagctgca gagcagggtc acggacgcct gcgagatggt acagctctcg ggcttcgaga
      541 agcgcaagcc gagcatgctc tccggcggcc agcagcagcg ggtcgcgctc gccagggcgc
      601 tcgtaaatcg tcccagggta ctgctcctcg acgagccgct cggggcgctc gacctcaaac
      661 tcagaaaaga gatgcagctc tacctcaaag atctgcagca cgaggtaggc ataaccttca
      721 tctacgtcac ccacgaccag gaagaggcgc tcacgatgag cgacaggatc gccgtgatga
      781 gcgagggcaa ggtccagcag gtcgcggacc cgcccacgct ctacgagcgc ccgaacaacc
      841 gcttcgtcgc ggactttata ggccagacca acgtgttctc cggcacggtc gaggccgtgg
      901 acggaggtcg ggtgacgctg agtgtcccgt caggagaaaa ggtcgaggcc tccacgggcg
      961 cggccgaggt cggggtcggg caggcgacac acgccgtcgt caggccggag aaggtcaggt
     1021 tcggtagcga gggcgacaac gtgatgaccg taaggatcca ccagatcgtc tacctcggcg
     1081 tcagcatcca gtacatagcc gacctgcccg gcggggagaa gttgattctc taccagcaga
     1141 acaccaccga ggaggaccac cccaaagtag gcgacgaggc accggtgtct tgggacgccc
     1201 gcaactgttt gatactagga ggatagactt ggcttttcgg agaaggatct cgcgggggga
     1261 tttcctacgg acgatgggcg cgggggtggt ggccggctcc acgctatccg tgctggcctg
     1321 ccagcccaac acaacggccc agaacagcgg tggcggcggc agcggcccgg aggagaagga
     1381 gctcaaccta tacaactggt cggattacgt ggccaagagc accctccctg acttccagaa
     1441 gcaaacgggg atcaaggtta cccaggactt cttctcgtcc aacgaggaac tcctggccaa
     1501 gctccaggcc gggggcaccg gctacgacgt gatcgtgcct tcagattaca tggtttcggt
     1561 catgatcaag agcgacatcc tggaacagct cgatatgtcc aagataccca acttcaagaa
     1621 cgttggcgcg aactacaagg ggctcggcta tgaccccgac aaccagtaca gcgtgcccta
     1681 ccagtggggc acgacgggca tcctctacaa caagaaaagg ctaggcacac tcgagccgag
     1741 ctgggacccg atgtgggatc ctgacaacaa gggcaagatc gggatgctca acgacgaacg
     1801 cgagacccct ggcgcagccc tctacaagct gggctactcc gtaaacgcta cggagaagga
     1861 acagctcgag gaggctgaag ccgaactcat aaagcagaag cccctgctcc gcggctactt
     1921 cgactccacg cagaacaggc catcggtgat aaacggcgac ctcctcctcg ggcacgtctt
     1981 ctcgggggac gccttcctcg cgctctcctc caacgaggac ctcgactacc tcatccccga
     2041 gcccgccgcg acccgctgga cggacaacat ggcgatcccg aaaggcgccg agcaccctga
     2101 caacgcgcac aagttcataa actacatcct cggcgccaag acgggggcca cgctctccaa
     2161 ctacacctac tacaacacgc ccaacgaggc ggcgctgccc atgatcgacg acgccctcaa
     2221 gaacctgccg ggctacgagc tggagcccag cgtcttcgac cggttgcaga tcatcgagga
     2281 cgtgggagcg gcgacccgcg agtactcgac cctgttcacc tcggtaaaga gctcctaggg
     2341 tatgggcaaa caagggcgcg gctggacggt ggggttggcg ggactgctct cgcctgtgac
     2401 cctctggctc gggctgttct tcctggtgcc gctgctgctc atcctggcct attcgttcgg
     2461 gaagagcggc atctacggcg gcataacgct cggcttctac ccgggcaact acctcaaggt
     2521 cttcgacccg ctgtatctgg agatcatcgt caggaccttc gtgatcgcgt tgttgaacac
     2581 gttcctctgc ctgctggtcg gctaccctct ggcttacttc ataaccttca aaggcggcag
     2641 gcacaagcac acgttgatcc tgctcgtcat gatcccgttc tggaccagtt tgctgctcag
     2701 ggcctacgcc tgggtcgtca tcctcggcgg cgacaacggc atcgccaaca gattcctaca
     2761 gttcttgcac cttacggacc acccgctgac gctcatcttc accccacagg cggtgattat
     2821 ggggatggtc tactcgtacc ttccgttcat gatcctgccg ctctacgcgg ccctggagaa
     2881 gttcgactgg cgcctcaaag aggcggccca ggacctcggg gcgagccggt ggcacacctt
     2941 ctggcgggtc accttccccc tcagcctgcc cggcgtcata gcggggagca tcctcgtgtt
     3001 catcccgtcg gcgggcgagt tcgtgatacc gaacctgctc ggcggggcga ggacggtgat
     3061 ggtcggtaac ctcatacagc agcagttctt acaggcccgc gactgggcct tcgggagtgc
     3121 gctcgcggtg atgctcgccg tcttgctggt cggggccatc atgttctacg tccgcagggt
     3181 cggggctgac aagctggagg aggtgtagac gatgggtgga gccaaaggtg gacccgtcta
     3241 tcgtggtgtc cagaaggttc ccgaccgggt cctgctcgtc ttcgccggcc tcgtctacct
     3301 gttcctctat gccccgataa tggtcctgat catcttctcc ttcaactcca ccaagaacac
     3361 ccaggtgtgg acggggttct cgacgcagtg gtacggggcg ctgatacggg acggttccgt
     3421 cctcaacgcg ttcaagctct ccctgacggt gggcctaacc gccacggcca tagcaaccgt
     3481 gataggaacg tttacggcgc tcgcgctctc gcgctacgag ttcaggggca agacgttcgc
     3541 cgactcggcc atctacgcag cgacagtgat gcccgagata gtcgtcggcg tcagcctgct
     3601 ggtcttcttc gtcgccgcaa cgattcctct cggcatcgtc acgatcgtca tcgcccacgt
     3661 ggccttctgc atctcattcg tcacgatagt ggtgcgggcg aggctctcgg ggatggaccg
     3721 ctctgtcgaa gaagcggcac aggatctggg cgcgaaccca gtccagacat tcctcagggt
     3781 aacgcttccg ctcatcctgc caggcgtcat ggcgggagcc cttctggcgt tcacgctctc
     3841 tttcgacgac ttcgtcataa ccttcttcgt cgcaggggtc gggtcgagca cgctgccact
     3901 caagatctac tccatgatca agttcggcgt cagccccgtg ataaacgccc ttagcacggt
     3961 ggtgatggtc tccacgatga tcctgatcct gggcggcggc cgtatcctgg cccgcaggca
     4021 gggaaacgct tcgtgacccc cgccatccgt cccctggagg cgaaggactt cgacgcctgg
     4081 cttccgctgt ggtacggata caattccttc taccgcaacg aggtagcagc tgaggttaca
     4141 gagaacacgt tccggaggct gcacgagaac acggacggct tcttcggcct ggtcgccgag
     4201 cacgacgggg gcctcgtcgg gctcgcgcac gccatcttcc atccctcgac gtggaccatg
     4261 aaaggctact gctatctgga ggacctgttc gtcgccaggt cccaccgcgg ctctggcgtc
     4321 gccagggccc tgatcgaagg cgtatatacg gaagcggacc gcaggggcgc agacagcgtc
     4381 tactggcaca cccagtccta caacgcccct gcgaggtccc tctacgacca ggtagcgcaa
     4441 tctacctcgt tcgtggtcta cgagagatag gcaacacgtg cgcgaataac ggcgagaagg
     4501 gctcttcgta caggccgaag aggacttccc tttctcgcgg gataaggagg gcaggtgcac
     4561 agcgtaggcg gtagcaggaa tgccggatct cctgaggttt cggagcacgg cttgaaggaa
     4621 aacgcgatcg ggttcagaga cgccctgatc atcggcatag cctcgacggc gccggcctat
     4681 tcgctggcgg cggttatagg gctggtcgtc gtggtcgtcg gcgtgcaggc accggccgtt
     4741 cttctggcct ctttcatacc gatgttcttc gtcgcaaccg cgttctacta catgaaccgt
     4801 gccgaccagg actgtggcac aacgttctcg tgggtaacga gggcgatggg accgtacctt
     4861 ggttggatcg ggggctgggc catctgcgtt acaggtatcc tggtcgtggg ctccctggcc
     4921 gacgtggccg cccgctacac gtacatcttt ttcggcctcg acgggctggc cggctccaag
     4981 gtggcgatca cgctctttgc cgttgtcatc atcgccgcga cgaccgccct ctgcgttatc
     5041 ggcaccgagc tctcggcccg catccaggac gttatgatca tcgcccaggt cggcgcgctc
     5101 ctgctcttcg ccgtcgtggc cctggtgagg gtctacgggg gaacggcgcc agaagggtcg
     5161 ctcgtcccga agctctcctg gttctccccc ttcgcggtgg acaccccgag cgccctcatc
     5221 gccgggctcc tgatcggggt ctttatctac tggggctggg agagctcggt gaacctcacc
     5281 gaggagacgg agaacagcga gtcagccccc ggcctcgccg ccgtggcaag cacggtgatc
     5341 ctgctcgtca cctacctgtc agtggcagcc gctgtggtcg gcttcggcgg cgtcgggacg
     5401 gcggagaggt tcgccgacga cgacgctatc ctaagcaccc tggcgaccgg ggtgctcggc
     5461 tcgccctggg acaagctggt cgtcctcgcg gtgcttacct ctgcgcttgc ctcgacccag
     5521 accaccatcc tgccggcctc gcgcaccgtg ctctcgatgg cgcgcgccaa cgcgctgccc
     5581 tccgccctcg gcgcgacgca cccgcgcttc ctgacgcccc acgtctccac catcgtcgtc
     5641 ggcgcgctcg ccacgctctg gtacgtgccg ctgaatttcc tctcggagaa cttcctgttc
     5701 gacacgctct cggccctctc gctcatgatc gccttctact acgccctcac gggcttcgcc
     5761 tgcgcgatct actaccgcca cgagctgttc aagagcgcca agaacttcct ctttatcggc
     5821 ctggcacccg tggtgggtgc cctggtcctc gcttatctgt tcttccgctc cataatcgac
     5881 ctcgccagcc cggatgcctc ctacaccggc gggtcctttc tcggcctcgg gttgccgctt
     5941 gttatcgggc tggggttctt gctgctgggc gtgatcctgg agatcatctg gcgcctcggc
     6001 ggccacggaa gctttttcgg acgccggccg gagaccgtat cgccggagat cgccgcaggc
     6061 agcgcccgcg tgaaagaggc cgccggcgcc ccgccggagg aggactagag gatgccggga
     6121 tgcttgatcc tcgggtacga cgacacaccg tcagcccgcg cggcgctgga tgtcgccatc
     6181 gagcaggcga aacggttcga cgagcggctg gtgatcctgt tcgcctacgc tcccccgggc
     6241 cgctcggtgg gcgaggagta cagcgaacac cgcagggtac tggaagagtt cggctcggtg
     6301 gcaacgagca aggcggcgga caaggcaacc gcggaggggg tcgatgtgga ggttctccta
     6361 gtcgccgaga ggccctctat agccctcgag aacgcggccc gcgcgcgggg tgcgcggctg
     6421 atcgtggtcg gaacctacgg cgaaagcccc ctgaagagcg caatccttgg cgccaccccg
     6481 cacaagctcc tgcagttctc cgagacgccc gtgctctgtg ttcccgcagg cccgcgctaa
     6541 agccagcccg ggccagcgct gttgcaccgt tttggcggca ttgttacact gagcttatac
     6601 ctgtactgta atcacaaggg aaagtgagag gcaacgggtt gctaggtggg gcaagtcgta
     6661 cctagcgggt ctggaggatc cgggggtgac cgaccttcgc atgcgcatcc agaccaatca
     6721 gggaagggag tgaggacgta tggcggagtc gatggggcga ggcgacgccc cgagggtaga
     6781 tggggtcgaa agaccgggtg gagatctcag ggacaaaggt ctccagcacg acgccgtggg
     6841 gctcctggcc agtgtagtgt tgggcgtctc ttgcgtggct ccggcctatg ctttgaccgc
     6901 gaccctcggt cccacggtaa gcgaggttgg gctgcagatg ccggcggtct tcctggcggg
     6961 tttcgtaccg atgttgctcg tggcgtacgc ctaccgtgag ctcaaccatg tggcaccgga
     7021 ctgcgggacc tcgttcacct ggaccgtgaa agcgttcggc ccccacatcg ggtggatgtg
     7081 cgggtggggc ttcgtgctgg ctacggtcat cgtgctctcc aaccttgtgg gcgtggcggt
     7141 cacgttcttc tacttgctgc tcgggcaggt ctttggcagc gaggctatcg ggacgctggg
     7201 cgacaacgcg gtcgtcaacg tgttgaccac cctggtcttc gtggcgggcg ccacggcgat
     7261 ttcctatcgt ggcatggcgg cgaccaaagg aattcagtac gtactggtgg gactgcagat
     7321 ggtcgtcctc gccctcttca tagtcctggc cttcggcggg gcgatcggcg gggcttccga
     7381 ctttgcgatc ggcttctcct ggggttggct caaccccttc gccatagaat ccttcgcggc
     7441 cttcgcggcc ggcctgtcgc tctccatctt catatactgg gggtgggaca ccaccctcac
     7501 cgtcaacgag gagacgaccg gcagctccag cacgccggga agggccgcgc tgctgacgat
     7561 cctaacgatc ctcggcacgt acttgctggt tgccatctcg gcccagatgt tcgccggcat
     7621 cggcaagcag ggcattggtc tgggtaatcc cgacacggca gacaacgtct tcgccgttct
     7681 ggccgggccc gtgcttggcg cgttcgccgt ctttctcttc ctcgcggtgg ttgcgagcgc
     7741 cgcgtctagc ttgcagacta cgttcttgcc agcggcgcgc acgatgctcg cgatgtcttt
     7801 ctacaaggcg gcgccggagc gtttctcgca cgtgcatccg cggtttcggt cgccgtcgta
     7861 cgcgacgttg ttctccggcg tggccacggc gatcttctac accgtcatga cgttcctcag
     7921 cgaggacgtc ctgatcgaca ccatctacgc gctcgggttg atgatctcct tctactactc
     7981 gcttacggcg ttcgcgtgcg tgtggtactt ccgcggggag ttgttcacga gccccagaaa
     8041 tttcctgttc cagggcctat tccccggcct tggcggcgtg atgctcacgc tcctcttctt
     8101 caagacggcc gtcgacacct tcgacccggc ttacggcagc ggcggggcca tcctcgggat
     8161 gggcaccgtc ttcgtcatcg gcgtgggcct gctgctcctc ggcgtcgtac tgatgttcgc
     8221 ctggcagcgt cgggcaccag agttcttccg cggggagacc ctcaggcacg acacggctgt
     8281 gctcacgaca gaagaggatc tgttggaaga tcgcgacccg gaggatggtt gagttggtcc
     8341 ggcatcccct ggatcccctg accgtcgagg agatccgcaa gacgacggcc atcctgcgtc
     8401 gcgacagaga gatgggcacc agatggcgct tcgcctccat cgagctgaag gagccgtcca
     8461 aagacacggt ggcaggttat tcgcctggcg atcctatctc gcgcgaagcg cgggtggtct
     8521 gctggaaccg tgaggacggt caggcctaca aggcggtggt ctcgctaacc gaggaccgcg
     8581 tcgtggcgtg ggagcatcgc ccgggcgagc agccgaacat caccgccgac gagttccacg
     8641 agtgcgacgt ggctttgcgc tccgacccaa gggtcgtgca ggtgctcgcc gaccgcggca
     8701 tcacggacct cgacaaggtg ctcttcgacg tctgggccta cgggggctcg ctcatcgcag
     8761 acaggtacag gggcctgcgg gtcggctggg cggacatctg gtatcgcgat cacgaggact
     8821 cgaacccata cgccaacccg gtgaacggcc tcaagctcat cgtagacctg aaccggatgg
     8881 agcttctgga gatagaggac gccttcagga tagagaagcc ggacgtgatg ggcgagtacc
     8941 tgcccaggta cgtgccgacg cccctgcgcg aggagccgag gccgctcgag atcacccagc
     9001 ccgaggggac ctcgttcgtg ctcgatggga acgagcttcg gtggcagaag tggtcgctgc
     9061 gggtgggctt caactaccgc gagggtctgg tgctccacac cgtcggctac gaggacggcg
     9121 ggcgcgtgcg gccggtggca caccggatgt cgttcgcgga gatggtcgtg ccctaccggg
     9181 atcccagccc cgagcactac aggcgcaccg cgttcgacgt gggcgagtgg ggcctggggt
     9241 acatgaccac ttcgctggag ctcggctgcg attgcctcgg ggagatccgg tacctcgacg
     9301 ccgtgctcag cgacgccgcg ggcgagccct acaccatcag gaacgcggtc tgcatccacg
     9361 aggaggacga tgccatcttg tggaagcacg tggacgacgt cgccggggcc gaggtccgca
     9421 gaagccggcg cctcgtggtc tctttccacg cgacggtggc caactacgag tacctcgtct
     9481 actggcgttt ctatcaggac ggcaacatcg agtgtgaggt gagggccacc gggatcatgg
     9541 tcaccacgtc gttcgaagag ggcgagcagc cgccctacgg ggccctagtc gacgagcgca
     9601 cctacgcgcc ctaccaccag cacttcctgg tggccagact cgacctggac gttgacggag
     9661 gggagaacac cgtttacatg tccgagtcgg aggccttgcc ggtcggaccg gacaaccccc
     9721 acgggcttgc gctggtccag cgcaacgttc cccttcgcac cgaggaggag ggcaaacagg
     9781 actacgactg gcagacccag cgttcctgga aggtggtcaa cgaaggcgcg agaaacggtc
     9841 ttgggacgcc aaccgcctac aagctggttc ccggtggttg ttttcccgcg atgctcgacc
     9901 catcctcgcc cgtactcggg cgggcgggcg tgatcgggca caccctctgg gttacgccgt
     9961 tccgagagga cgagcgttgg ccctgcggcg agttcgtcaa ccagagcgag gaagacacgg
    10021 gcctaacgcg gtggacccgg cagaaccgcc ccatcgagaa caccgacgtg gtgctgtggt
    10081 acgtgttcgg cctccaccac atcacgcgcc tcgaagagtg gccggtaatg cccgtggaca
    10141 ccgtatcttt ctggctcaag ccaaccggct tcttcgaccg aaaccccgcg ctcgacgttc
    10201 ccccgagcag gcattgccac gacccggtcg gggatacccc gtgatagatc cactccagcg
    10261 ctgggcctct gttggggaga agccggatta cgccggtccc ctgtcctacg gcggcgtccc
    10321 ctacacgcag gatgcggtct ctctcgaagg cgcggacgtg gccatcgtgg gcgccccgat
    10381 ggacgagctc gtctccgatc ggcccgggac gcgcttcgcc ccgcgcgcca tcagggcggc
    10441 gagctgcccg ccggggccgc agctcgagtc cggtgtggac gcgctcaccc gccttcgtgt
    10501 ggtcgactac ggcgacgcgc ccatcctgcc cgctgacccg gttcgttcgc acgccgccat
    10561 aaaggagacg gtagggcagg tactggcagc aggcgcaatc ccggtggtgc tgggcggcga
    10621 ccattccatc accgagcccg gggtgcgcgc ctgcgtgggg gcgcacggcc ccgttggcct
    10681 cgtgcacttc gacacgcaca ccgacaccgg ccgcgaggtc ttcggcgcgg ggctctcgca
    10741 cgggaccata atgcgcaggc tcgtggagga agggagcgtg gatccggaac gctacgtcca
    10801 gatcgggctg cgcggctact ggcccggtga agaagagttc acctggcaaa aggaccgcgg
    10861 catcacgagc ctcttcatgc acgaggtaag agaccttggg atagaggagg tcacggaacg
    10921 cgccatcgag gtggtcggtt ggggtccggt ctttctaacc gttgacatcg acgtgcttga
    10981 tcccgccttc gcgccgggca cggggacacc tgagccagga gggatgacca cggcagagct
    11041 gctcctggcg gtgcgcgagg tggcgcggca gctggacgtg gtcggggccg acatggtaga
    11101 ggtgataccg acgggcgtgg ggtcagcgga cgtcacagcg ctcgccgcag ataggatagt
    11161 gcgcgagatc ctcggcggca tcgcgcagca tcgcaaagaa aaggaaaggc aacagaagga
    11221 ggaagcatga ccgaaacgcg gacgctccag aactttgttg gtggcgagcg ggtggagccg
    11281 gttgacggcg gcacctacga cctgctgaac ccggccacgg gcgaggtgtt ctcccaggcg
    11341 ccgctctccg gcgaggagga cgtcgaccgg gcctaccggg cggcggagcg ggccttcgag
    11401 acctggcgcg agacgacccc ttccgagcgt cagctggccc tgctcaggat tgccgacgcc
    11461 atagaagagc gggccgagga gatcgtgcgc gccgagtccg agaacacggg caagcccttc
    11521 gggctcacca tggaagagga ggtaccggcg ggggtagatc agatccggtt cttcgccgga
    11581 gccgctcgcg tcctggaggg caagtcggcg ggcgagtacc tggaggggta cacctccttc
    11641 atccggcgcg agccgatagg cgttataggc caggtaaccc cctggaacta ccccatgatg
    11701 atggccatct ggaagttcgc cccggccatc gccgccggca acaccatcgt tataaagcca
    11761 tcggacacca ccccgatctc gacggttatg atggccgaga tcgcctccga gttcctaccg
    11821 gagggcgtct tcaacgtggt gtgcggcgac agggacacgg gccgggcgct ggtccagcac
    11881 ccgacgcccc agatggtggc cataacgggc tccgtcaggg ctgggatgca ggtcgccgaa
    11941 gcggcttcct acgacctcaa gcgcgtgcac ctcgagctcg gcggcaaggc cccaataatc
    12001 agcttcgacg acgccgacat cgaggaggcc gccgaggcca tcgccggggc cggttacttc
    12061 aacgccggtc aggactgcac cgccgccacg agggtacttg cggccccccg catctacgac
    12121 gacttcgtgg acgccatcac cgagcaggcg aagaacacaa ggacgggcga tccaaacgac
    12181 gaggacaccg actacggccc cctgaacaac tccaaccagc tcgcacacgt ctccggcatg
    12241 gtggagaggc tccccgacca cgccgaggta accgtcggcg gtgagcgctt tggggacagg
    12301 ggctacttct actccccaac ggttgtctcc ggcctcgagc aggacgacga ggcctcccag
    12361 aacgagatct tcggccccgt catcaccgtc cagcacttct cagaggagga cgaagccgtc
    12421 cgctgggcca acggcgtcaa gtacggcctc gcctccagcg tgttcaccag agaccacggc
    12481 cgcgccatgc gggtcagccg caggctggat ttcgggtgcg tctggatcaa cacgcacatc
    12541 ccgctcgtgg ccgagatgcc gcacggcggc ttcaagcact ccggctacgg caaggacctc
    12601 tccatgtacg gcctggagga ctacacccgc atcaagcacg tgatgagcaa cataggggca
    12661 taaggaaggt atcaggctac aggcatcagg caatgggcat caggtgtttt ccggcagcct
    12721 gtaacccgtt gcctgatgcc tgctttttcg gagtggctta tcctcttcca gcaccgtcat
    12781 agatagggac gagcccatga cgacctgcgg agaagcattg gtcgggctgc tggaggccta
    12841 cggcatcgac accgtgttcg gcatccccgg cgtgcacact gtggagctct accgcggcct
    12901 gccggacaca cgcctgcgcc acgtcacacc gcgccatgag cagggcgcgg gctttatggc
    12961 cgacggttac gcgagggtct gtggccggcc ggcggcctgc tttgtcatca ccggccccgg
    13021 cctgaccaac atagccacag ccctgggaca ggcctacgcc gactcggttc cgctgctggt
    13081 gatctccagc gtgaacaaca ggcacgaact gggcctgggc gaaggaaggc tgcacgagct
    13141 gccctcccag cgcaacctga tggccggcct gaccgcgttc agccataccc tgcaacgccc
    13201 ggacgagctg cccgcggtgc tggcgcgcgc cttcgcggtg tttgacgccg ccaggccgcg
    13261 tccggtccac atcgaagtgc cgctggacgt gatcacagcc cccgccgacc acgtccagaa
    13321 gcagagggcg gcgttcgtct cccgaccggg acccgccgga gaagcggtcg ccgcggccgc
    13381 cgagctcttg cgcggggccg aacgtccgct cgtggtgctg gggggcgggg caagcgaggc
    13441 ggccccccag gcgcgcgccc tggtagagcg gctcggggcg cccgtggtaa ccaccgtcaa
    13501 cggcatgggg atagtgccct tcgatcatcc cctgtgcacc ggcagcacgc tctcccagcc
    13561 cgtggtgctg gaagaactgg cccaggccga cgtcgtgcta gccgtcggca ccgaactcgg
    13621 cgagaccgac acgctgctgt tcgatgggcg tcccgcactg aacgggcgtc tgatccgtgt
    13681 cgacatcgat ccggggcaac tcgccggcaa cgcgctgccc gacgtcgcca tcctgggcga
    13741 cgccggccct gctcttgagg cccttgagcg tgagttgagt ggccacgagg cagacacgac
    13801 cgcggcggaa gcacgggcga ggacgctgcg cgaactgtcg caacggcgcc tggacgggcg
    13861 gcagcgtctg caccagcgct ttgtggcggc ggtgcaggaa gccctgcccg gcgtgatcct
    13921 ggtgggtgac tccacccagc cgatctacag cgcaaacatg ttctacgggg ccgggcgccc
    13981 gcgctcgtac ttcaactcca gcacgggcta cggaacgctg ggctacgcgc tgccggccgc
    14041 cctgggcgcc gggctcgcca gaccggaacg gccggttgtg gcgctccttg gcgacggtgg
    14101 gttacagttc acggtcggcg aactcgcgac cgccgtggag ttggagatgc cgatcccaat
    14161 cctgctgtgg aacaaccacg gctacgcgga gatagaacgc tacatggtgg agcgcggcat
    14221 accgcaggag ggggtcgaca tatacacgcc ggatttcctc gccatagccc tcggcttcgg
    14281 ctgccgcgcc gagcgggccg agggcctgga gcacctgggc gcgctgctgc ggcggtccgt
    14341 ccttgaggcg ggaccgaccc tgatcgaggt cgacgagaga gaggcgctcg cgtggtagcg
    14401 ggggcgcaag caacggagag ggagatgatt ccgtgaacct gctcgactac ctgatcatgc
    14461 tgctctactt cgcggtgatg ataggcgccg gctactgggg gctcacgagg gcccgcagcg
    14521 ccgacgacta cctcgttgcc gggcggaggt tgggcccgtt catgtacatc ggctgcctga
    14581 gtgcggtggt gctcggcggc gcctcgacca tcggcggcgt ggccctcgga tacgagaacg
    14641 gcatctcggg catgtggctc gtgttctgga tcggcatggg cgtcatcgcc ctgggcctcc
    14701 tgatgtccac ccggctctcg cgcctcggcg tctacaccgt ggccgagatg ctagagaacc
    14761 gctacggggc accctcgcgc ctgatcagcg cgctcatcat cgccgcctac gccctcatga
    14821 tagccgtgac ctccaccatc gccataggga ccgtcttcaa ggtggtcctc ggcctctccc
    14881 ccacagtggc gatcctggtg gccggcggga tcgtcgtcgc ctactctgtg gccggcggta
    14941 tgtggtcgat cacgctcacg gacatcatcc agttctgcat catgacggtc ggcatcttct
    15001 tcctact
//
LOCUS       DBNGVD010000331         1547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1169049, whole genome shotgun sequence.
ACCESSION   DBNGVD010000331 DBNGVD010000000
VERSION     DBNGVD010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1169049"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC16CM_08885"
     CDS             complement(<1..660)
                     /locus_tag="AC16CM_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR3139988.1"
                     /translation="MVTQFLIALLVTSAFAALAYALGMVSRGGALGGLLVGTIVYACL
                     GPQGFAVLALFVVVGSLLTRIGYRSKQRAGTAQEHGGRRGVRNALANCSVAVLCAILA
                     WATGYDPLTAAFVASLGAAFADTAESEIGQLFSRAPRLITTLRKVPPGTDGAISLPGT
                     LAGIGAALLTAALALILGLLASPAPALLVATAAFLGTIADSLIGNLLPRAGNELTNIL
                     CT"
     gene            complement(654..1109)
                     /locus_tag="AC16CM_08890"
     CDS             complement(654..1109)
                     /locus_tag="AC16CM_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3139989.1"
                     /translation="MSEATVGILVDYESPLASRVASEVWSGLSRTALEAGFTKTTALW
                     HSLREVSDGRSPENSSAHIFVLGQDRPEAIDAIAAMDAPAPHLYGVIVAVPVEQSPLA
                     GSLLYQGVGRLTQGGVKAGMVEPALLHAVPTECERYARHLFGRLSKVGW"
     gene            complement(1136..1471)
                     /locus_tag="AC16CM_08895"
     CDS             complement(1136..1471)
                     /locus_tag="AC16CM_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3139990.1"
                     /translation="MNASRSDMFRKLLERDPNNPMVLYSLGSELFKEGQYPEARDLLR
                     RAVENKPDYSVAYRMLGRAHFELHENSEARSVFLRGKQVAQENGDLQTVKEIDVFMRR
                     LEKRGVGEE"
BASE COUNT          258 a          477 c          530 g          282 t
ORIGIN      
        1 cgtgcatagt atgttcgtaa gttcattacc ggcgcggggt aataggttcc cgatcaggct
       61 gtcagcaatc gtgcccagga acgctgctgt cgctacgaga agggccggag ctggtgatgc
      121 gagcaaaccc aggattagcg cgagggcggc ggtaaggagt gccgccccga taccagcgag
      181 ggtgcctgga agggagattg ctccgtccgt gcctggcggt actttcctca gggtggtgat
      241 cagacgcggc gcgcggctga agagttggcc tatctcggat tccgccgtgt ccgcaaaggc
      301 cgcgccgaga gaggcgacaa aggcggccgt gaggggatcg tagccagtgg cccaggcgag
      361 gatggcgcag aggacggcga cggagcagtt ggccagtgcg ttgcgtacgc cgcgtcgtcc
      421 cccgtgctcc tgcgcggtgc cggcgcgttg cttgctgcgg tagccgatcc gcgtcaggag
      481 ggagccgacg accacgaaga gggcgaggac cgcgaagccc tgtgggccga ggcaggcgta
      541 gacgatggtc ccgactagaa gcccgccgag ggcaccaccc cggctgacca tcccgagcgc
      601 gtaggccagc gcggcgaagg ccgatgttac cagtagggct atcaggaatt gcgttaccaa
      661 cctaccttcg agaggcgccc gaagagatgg cgggcgtagc gctcgcactc ggtggggacg
      721 gcgtgcagga gggccggctc caccatcccc gccttcacgc cgccctgcgt caggcgtccc
      781 acgccctggt agaggaggga ccccgccagg gggctctgct cgacgggtac ggcgacgatc
      841 acgccgtaca ggtgcggcgc cggggcgtcc atggcagcta tggcgtctat cgcctcgggg
      901 cgatcctggc cgaggacgaa gatgtgggct gacgagtttt ccggggacct gccgtcgctg
      961 acctcccgaa ggctgtgcca gagcgctgtg gtcttcgtga acccggcttc gagcgcggtt
     1021 ctggagagcc ccgaccatac ctcgcttgcc accctgctgg cgagcgggct ctcgtagtcg
     1081 accaggatgc ctaccgtggc ttccgacacg ccggggatgt tagcagaatc gggggctact
     1141 cctcccccac cccgcgcttc tctagccggc gcatgaagac gtcgatctcc ttcaccgtct
     1201 ggaggtcgcc attttcctgt gccacctgct tgcccctcag gaacacgctt ctggcttcgg
     1261 agttctcgtg cagctcgaaa tgggcacggc ccagcatcct gtaggccacg gaatagtcag
     1321 gcttgttctc gaccgcccgt ctcagaagat cccgggcctc cgggtactgg ccttccttga
     1381 agagctcgct cccgagcgag tagaggacca tcgggttgtt cgggtccctc tccagtagtt
     1441 tgcggaacat atccgagcgg gaagcgttca tggcctgttt ttagcacgcg ggcgtgctat
     1501 ctggtagatt cggccatcgt atctttcagt gctagagtct ggactgt
//
LOCUS       DBNGVD010000332         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3538220, whole genome shotgun sequence.
ACCESSION   DBNGVD010000332 DBNGVD010000000
VERSION     DBNGVD010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3538220"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="AC16CM_08900"
     CDS             <1..1083
                     /locus_tag="AC16CM_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050526 - poly(3-hydroxybutyrate)
                     depolymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular catalytic domain type 1
                     short-chain-length polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR3139991.1"
                     /translation="GSKGTGDGPIDITSRVVKRAGQGPADGRFGALRRPNTTARSTPQ
                     PNPLTRGMAGMAGAKARSASSASVPDGARFIEGSYGNGAGTRSYKLYVPGGYSGHAVP
                     LVVMLHGCTQDPDDFAAGTRMNELAEEHTFLVAYPAQSGNANMQKCWNWFQATDQQRG
                     RGEPSIIAGITKQIVDEYEVAEDRVYVAGMSAGGAMAAIVGATYPDLYAAVGVHSGLA
                     PGSAHDMSSAFTAMRQGGPVVATQNGAGGDNRKALPTIIFHGDGDRTVHPRNGERLLG
                     HLDSGTRNGSAPRVTTRRGGVPGGHEYTKFTYQDGEGRNLVERWSVHGLGHAWSGGSY
                     PGSYTDPRGPDASAEMVRFFLQHQKS"
     gene            complement(1127..2119)
                     /locus_tag="AC16CM_08905"
     CDS             complement(1127..2119)
                     /locus_tag="AC16CM_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3139992.1"
                     /translation="MTGLTVLAILALVVFLVAARSIRIIPQSRVAIIQRLGRYHRTAE
                     SGLTFVVPVVDRMLPKTDLREQVIAFQPQAVITNDNVGMQISTVVYYQVVDARASEYE
                     VANFGLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDDVTEKWGIRVTRVE
                     LKEIIPPRDIQQAMEKQMQAERTRRASILTAEGDKRSAILKAEGEKESSILRAEGEQR
                     SAVLRAEGEAEAYRNVQAAQIEMTGKLFERLEGSDLSPESLRYLYLKALPEMAQGPAS
                     KLFVVPSEIQDLAGTIGALAGGASLANENSKGEGEQRSLKAPTAGPNGELPTRG"
     gene            complement(2116..2577)
                     /locus_tag="AC16CM_08910"
     CDS             complement(2116..2577)
                     /locus_tag="AC16CM_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3139993.1"
                     /translation="MDWEIIFWAALAALAFVGEVLTVSFFLLFFSLGAVVGLVAAMFD
                     LSLAAQVMGFVLASLLSMAVLRPALLNRLSLRGSEGYVGHRGITGERAVVTEEIEAGG
                     KGMVRVGSGEFWTARSLHPEEKIERGTRVRVLDTDGLTALVDIVHTKEERI"
BASE COUNT          471 a          867 c          860 g          455 t
ORIGIN      
        1 gggtcgaaag gtactggcga cggtcctata gacatcacct ccagggtcgt aaagagagca
       61 ggccagggtc cggcggacgg gaggttcggg gccctccgca ggcccaatac gaccgcacgg
      121 agtaccccgc agccgaaccc gctcacgcgc ggcatggcgg gtatggcggg ggcgaaggcg
      181 cgttcggcgt catcggcaag cgtgcccgat ggagcgcgtt tcatcgaggg gtcctacggc
      241 aacggtgcgg ggacgcgctc gtacaagctc tacgttccgg gtggttactc cggtcatgcg
      301 gtgccgctgg tggttatgct tcacggctgc acccaggacc ccgacgactt cgcagcgggc
      361 acccgcatga acgagctcgc cgaagagcat accttcctcg tggcatatcc ggcacaatcc
      421 ggcaacgcca acatgcagaa gtgctggaac tggttccagg ccacggacca gcagcgcggt
      481 cggggcgaac cgtccatcat cgccggcatc acgaaacaga tcgtggacga gtacgaggtc
      541 gcagaggacc gggtgtacgt ggcaggaatg tcggcgggag gggcgatggc cgcgatcgtc
      601 ggggcgacct atccagacct gtatgccgcc gtcggcgtac actccggcct cgcccccggc
      661 tctgcccacg acatgtcctc ggccttcacg gccatgcgtc agggcgggcc cgtcgtcgca
      721 acgcagaacg gcgcaggtgg ggacaatcgg aaggccctac ccaccatcat cttccacggc
      781 gatggcgaca ggaccgtcca tccacgcaac ggtgagcggc ttctaggcca ccttgactcc
      841 ggaacccgga atggctcggc accgcgggta accacccgtc ggggtggcgt gccaggcggt
      901 catgagtaca cgaagttcac ctaccaggac ggggaaggcc gaaatctcgt agagcgttgg
      961 agcgtgcacg ggctcggcca cgcctggtcc ggcgggagct accccggctc gtacacggac
     1021 cccaggggtc cagacgcctc agccgagatg gtgcgcttct tcttgcagca ccagaagagt
     1081 tagccgaggc gattcgggac gccggagcca gaccctacag ggggtttcac ccccgtgtcg
     1141 gaagctcgcc gttgggcccc gcggtcgggg ccttcaggct acgttgctct ccttcgccct
     1201 tcgagttctc gttggcgagg gaggcaccgc ctgccagggc cccgatggtg ccggcgaggt
     1261 cctgtatctc ggaggggacc acgaacagtt tgctcgccgg tccctgggcc atctcgggga
     1321 gggccttcag gtagaggtag cgcagggact cgggggagag gtcggagcct tcgaggcgct
     1381 cgaaaagctt gcccgtcatc tcgatctggg ccgcctgcac gttgcggtag gcctccgctt
     1441 cgccttcggc gcggagcacg gccgagcgct gctcgccctc ggcgcggagg atggaggact
     1501 ccttctcgcc ttcggccttc aggatcgcgg agcgcttgtc gccctccgcg gtcaggatcg
     1561 aggcgcggcg ggtccgctcg gcctgcatct gcttctccat cgcctgttgg atgtcgcgcg
     1621 gggggataat ctccttcagc tccacgcgcg tcaccctgat gccccacttt tccgtaacgt
     1681 cgtcgaggac cgtgcggagc ttggcgttga tctcgtcgcg ggaggtcagg gtcttgtcca
     1741 ggatcaggtt gccaataacg ttgcgcaggg tggtctgggt gatctgctcc agggccaggc
     1801 cgaagttggc cacctcgtac tccgaggccc tggcgtcgac gacctggtag tagacgacgg
     1861 tggagatctg catcccgacg ttgtcgttgg tgataaccgc ctggggctgg aacgcgatga
     1921 cctgctctct caggtccgtc ttgggcaaca tcctgtctac caccggcacg acgaaggtta
     1981 gcccgctttc ggcggtgcga tggtagcgcc cgagccgctg tatgatcgcc accctgctct
     2041 gggggatgat cctgatgctg cgcgccgcca ccagaaagac cacgagcgcc agtattgcca
     2101 acactgtgag gccggtcata ttcgttcctc cttggtatgc acgatatcca ccagcgcggt
     2161 aagcccgtct gtatcgagga ccctcaccct ggtgccgcgc tctatcttct cttcgggatg
     2221 gagggagcgg gcggtccaga actccccgct tcccacacgc accatcccct tcccgccggc
     2281 ttctatctcc tccgtcacca ccgccctctc gcccgtgatg cccctgtgtc cgacgtaccc
     2341 ctcagacccc cgcagcgaga ggcggttcag gagggcggga cgcaggacgg ccatgctgag
     2401 caacgaggca aggacgaacc ccattacctg ggccgcgagg gacaagtcga acatggccgc
     2461 gacgagcccg acgacggcgc ctagagaaaa gaagagcaag aagaaagaga cggtgagtac
     2521 ctctccgaca aaggcaagag ccgccaacgc ggcccagaag atgatttccc agtccatctc
     2581 gtatctcctg tcatgagttt acaccaccat cagcgaccag aaaccagacc ccgttcacaa
     2641 ctcgggccta caa
//
LOCUS       DBNGVD010000333         3753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3902674, whole genome shotgun sequence.
ACCESSION   DBNGVD010000333 DBNGVD010000000
VERSION     DBNGVD010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3902674"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="AC16CM_08915"
     CDS             <1..1050
                     /locus_tag="AC16CM_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3139994.1"
                     /translation="AIRWLLSTVPSAMIFDDHEVGDDWNISQAWVEEMRQHPTWNDQI
                     VGGYASYWVYQHLGNLPPEELQRDDLYSRARTAEDAWPLLHEFAYKAHRGPDGARWSY
                     HRDLGNTRLIMIDSRGGRVLQEDRRSMVDAEEWAWIEDMATGGFDHLMIGTSLPILLG
                     PGMHHFQSWDEKLCSGAWGRRAARWAEGFRRSQDLDHWASFHDSFAAMVDLIDEVAGG
                     KRGRPPSTVLILSGDVHHGYLAEASLGNGTESQIYQAVCSPLRNALPKQKSFLQANAW
                     TGLASLAGRVLTRLARVPAEPISWSMTHDAAFFENQIATLELDERSATITFEGAVLGA
                     AKEPSLERLYRYRLA"
     gene            complement(1517..1864)
                     /locus_tag="AC16CM_08920"
     CDS             complement(1517..1864)
                     /locus_tag="AC16CM_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3139995.1"
                     /translation="MSEGSGRQETERRLIQRSLEDDVFRQQLLTDPRAIIERELGTRL
                     PEGVRVEAVEETQDTIYLVLPSASPPGEGAELSDRELEAVAGGGWSQDTVNDGTCAAG
                     ATCQPCFTWSACE"
     gene            2101..2499
                     /locus_tag="AC16CM_08925"
     CDS             2101..2499
                     /locus_tag="AC16CM_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3139996.1"
                     /translation="MSPKSPLKVMLVEDHAAFRQALVFVLKSEQGLEVVAQAGSLAEA
                     RKALGDEGGLGGRLDVALIDLLLPDGEGTELVGELRRANPSIKVIVLSADLWPWRIRE
                     LSRAGVDAVLDKVQSPRQIAREVRREGGGG"
     gene            2688..3526
                     /locus_tag="AC16CM_08930"
                     /pseudo
     CDS             2688..3526
                     /locus_tag="AC16CM_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788909.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          687 a         1215 c         1192 g          659 t
ORIGIN      
        1 gcgatacgct ggctgctctc caccgtgccg tcggccatga tcttcgacga ccacgaggtc
       61 ggcgacgact ggaacatctc gcaggcctgg gtggaggaga tgcgccagca ccccacctgg
      121 aacgatcaga tcgtcggcgg atacgcctca tactgggtct accagcatct gggcaacctc
      181 ccccccgaag aactacagag agacgacctc tacagcagag caagaaccgc cgaagatgcc
      241 tggccgctcc tgcacgagtt cgcctacaaa gcccaccgcg gccccgacgg tgcgaggtgg
      301 agctatcatc gcgacctcgg caacacgcgc ctaattatga tcgactcccg cggcggcagg
      361 gtactgcagg aggaccgccg ctccatggtg gacgccgaag agtgggcctg gatcgaagac
      421 atggccaccg gcggcttcga ccacctcatg atcggcacct cgcttcctat cctcctcggc
      481 cctggcatgc accacttcca gagctgggac gaaaagttat gctccggtgc ctggggcaga
      541 cgcgccgcgc gctgggcaga agggttccgc cgctcccagg acctcgatca ctgggcctcc
      601 ttccacgact ccttcgccgc gatggtcgac ctgatagacg aggtggccgg tggaaagaga
      661 gggcgcccac cctccaccgt cctgatcctc tccggcgacg tccaccacgg atacctggcc
      721 gaagcttcgc tcggtaacgg cacggagagc cagatctacc aggcggtctg ctcccccctg
      781 cgtaacgccc tcccgaaaca gaaatcgttc ctgcaggcca acgcctggac aggcctcgcg
      841 tccctcgcgg ggcgtgtcct gacccgtctg gcccgcgtgc cagcagaacc gatctcctgg
      901 agcatgaccc acgacgccgc cttcttcgaa aaccagatcg ccaccctcga actcgacgag
      961 aggagcgcca ccatcacgtt cgaaggagcc gtccttggtg ccgcaaagga accgtccctg
     1021 gagagactct accgataccg tctggcgtaa atcgctctgc ttcgcgtgcc gtcccgccaa
     1081 ctctcagccg ttggcttttt acccatacct ggggacttcg gtccacaatc cccgcgcaag
     1141 tagaggagtg tggttcggcg gaactctgtt cgtagccaga ttctccctca cgcggcgtct
     1201 ccccgaaacc tgtgcacgct ctcgcatcgt tctacctgag cacgacatat tgtggcagcc
     1261 tctggtgtgg tgggaggcgg gtagcatgcc ctcgagagca ctcggcaccg gtctatacag
     1321 gaccctgatt cgaaatgcgg agatttcacc ttccccaaag tcgggttaat agagcgctgc
     1381 ttcgtcttat cgtggggcgc ttcggaaccg gcgcgagctc attcgggccg gtgtgagcca
     1441 acaagcaacg agctacaggt tcactctttc cgacgctagc gctgacgcgg ctcggatgcc
     1501 accacggtac gctacatcat tcacaggcag accaggtaaa gcaaggttgg caggtggcgc
     1561 ccgcggcgca ggtgccgtcg ttaacggtat cttgcgacca tccgccgccg gccaccgcct
     1621 ccagctcccg gtcggagagc tcggcgcctt ctccgggcgg agaggcgctg ggaaggacca
     1681 ggtagatggt gtcctgcgtc tcctccaccg cctccaccct gaccccctcg ggcagcctcg
     1741 tccccagctc tcgctctatg atcgccctgg ggtcggtgag cagttgttgc ctgaagacgt
     1801 catcctccag gctcctctgt atgagcctcc gctccgtctc ctgacggccc gacccttcgc
     1861 tcatgggtgc tttccctttc gttctcgtcc catcctgccc tggtgctcgg ctggtcccgg
     1921 ttctgcgacg ctgacatggt agcaagcgcg aggttgtagg tacctctacc aggtagttac
     1981 ccttggaacg ccgcaaagcc gaagttaggg gactacgcca ctgcaatgca cccccgggag
     2041 aacgggaacc gaagactatc tcatcgcgct ccgtcgagtc gtgctttact gaaggacacc
     2101 atgagcccca agtctcccct gaaggtcatg ctggtcgaag accacgccgc cttccgccag
     2161 gccctcgtct tcgtgctgaa gagcgagcag gggctagagg tggttgccca ggccggctcc
     2221 ttggccgagg ccagaaaggc gctcggcgat gagggaggtc tcggggggcg cctggacgtg
     2281 gcgctcatag accttctcct gcccgacggg gagggcaccg agctggtagg cgagctgcgc
     2341 cgagccaacc cctcgatcaa ggtgatcgtg ctgagcgcgg acctgtggcc ctggcgcatc
     2401 cgcgagcttt cgagggctgg ggtcgacgcc gttctggaca aggtgcagtc ccccaggcag
     2461 atcgcccgag aggtaaggcg cgagggggga ggggggtgaa gccctccttg tggggcacga
     2521 ggaggagcag gatggtgccg catatcccgg cttcctcgaa gccgccacac gccaacttcg
     2581 gagaatttac cttctagaga ctgttttgca aacgatgatc ggcgtgcttg tgagccgctg
     2641 ggaggctacg atcagggcgt acgactcccg agtttccgga ggcctccatg tcgcgtacac
     2701 gtcgccggta cccctccgac ctctccgacg cggagtgggc cctgctggag cccctactcg
     2761 cgtcctccga gaggcgaggt cgcccgccga agtggcccgc caagcgcgtg gccgacgccg
     2821 tcttctacct gctaaggagc ggttgcgcct ggcacatgtt gccccgggag ttccctacac
     2881 aaggcagacg gtgtactacc acttctacaa gtggcgtcgg gacgggaggc tcaggcgagc
     2941 ccacgatcgg ctgcgcgaag cggtccgaaa ggcggagggg cgcgagcgca acccgagcgg
     3001 agcggtgatc gacagccagg ccgtcaaagg gaccggggtg ggaggccccg aacgcggcta
     3061 ttgagtaggg gcgaagcggc tctcgggcag gaagcgccac ctgctcgtgg acaccggagg
     3121 gctcgtgctc ggcgcgcggg tccacgccgc cagcctgcac gaccgcgatg gcgcgcgggt
     3181 gctcttgagt gaggagctca aggaagagtt gccgcggctg gagttgttgt gggcagacgg
     3241 cgcgtacacg cgcgggtttc gcgagtgggc caaggatgag cgggggtggc gggtggaggt
     3301 gccgcatcat cgcgaccggc agttgtggcg ctacgggctg gaggagaaac cccgcggctt
     3361 tctggttttg ccgaggagat gggtggtcga aaggaccttc gcctggctgg gccacgctcg
     3421 acgactcgcc aaggattacg agcggttgcc ggagacaggg gtggcgatga tccactgggc
     3481 gatgagccgc atcatgctgc gtaggctgac cggtgcgaca cgctgaaaaa atgtccagaa
     3541 aacactccaa gacgcctaag gaaagcgact tcggggacta ttgcaaaaca gtctctagaa
     3601 gggttattcc gcgaagttcg tctaaagaag tggccactcg gcatacgaag atatgccggg
     3661 tggcgtatat gcagcgcccc gcccctacaa tccgatcaat actcaagccg ggcgcctact
     3721 ctacctcctc ccgagagcgc ggcccggctt ttc
//
LOCUS       DBNGVD010000334         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2081038, whole genome shotgun sequence.
ACCESSION   DBNGVD010000334 DBNGVD010000000
VERSION     DBNGVD010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2081038"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..851
                     /gene="tpiA"
                     /locus_tag="AC16CM_08935"
     CDS             69..851
                     /gene="tpiA"
                     /locus_tag="AC16CM_08935"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3139997.1"
                     /translation="MARRPMMAANWKMNKTIREAEDYTAALLPRAADAEGVDVAVFVP
                     FTCLHEVGRMSEDSPVVAGAQNFYFEDSGAFTGEISAPMLLDVGAGAVIVGHSERRET
                     FGETDDAVAKKTRRALEADLLPVVCVGETKEERDAGGMWDKVSGQVSRVIEELDDVDG
                     ESIVFAYEPIWAIGTGDTATPEDAQDAIGKIRDLLRQEVGDDFADAVRILYGGSVKPG
                     NAAEIMAQDDVDGGLVGGASLEVDSFVELIEAAQNAVAETEQ"
     gene            873..1103
                     /gene="secG"
                     /locus_tag="AC16CM_08940"
     CDS             873..1103
                     /gene="secG"
                     /locus_tag="AC16CM_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3139998.1"
                     /translation="MLLYYLIAFFQIVFCVALVGLILLHSGKGGGLSSSLGGGMGSTF
                     SGTTIMEKNLTRLTIIVLGLFALTNIALFFLA"
     gene            complement(1238..1322)
                     /locus_tag="AC16CM_08945"
     tRNA            complement(1238..1322)
                     /locus_tag="AC16CM_08945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Leu,seq:gag)
     gene            1404..>1770
                     /locus_tag="AC16CM_08950"
     CDS             1404..>1770
                     /locus_tag="AC16CM_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3139999.1"
                     /translation="MGRNRFLLVALAALSLIVALCLRRGRRSMIARDWPVNLAHRGAS
                     ASAPENTLEAFRLAERSGAGGIELDVHLTHDGYPVVIHDPTLDRTTNLTGAVAEMGLD
                     EVRGADAGYCFSTDGGKTYP"
BASE COUNT          335 a          534 c          585 g          316 t
ORIGIN      
        1 ctggagtacg ttgaaggcaa agagctgccg ggcgtcgccg tactgcccgg caagtagagg
       61 aggagaagat ggcccgacgc ccgatgatgg cggcgaactg gaagatgaac aagaccataa
      121 gggaggccga ggattatacg gccgccctgc tgccgcgtgc cgctgacgcc gaaggggtgg
      181 acgtggccgt tttcgtgcct ttcacctgcc tgcacgaggt aggcaggatg tccgaggatt
      241 ccccggtagt cgctggcgcg caaaacttct acttcgaaga ctcgggcgcc tttaccggcg
      301 agatctccgc gcccatgctc ctcgacgtgg gggccggggc cgtgatcgtg ggtcactccg
      361 agcggcgcga gaccttcggc gagaccgacg acgcagtcgc caagaagacc aggcgtgccc
      421 tggaggccga cctgctgccc gtcgtctgtg ttggcgagac caaggaggaa cgcgacgctg
      481 gcggcatgtg ggacaaggtc tccggccagg tctcgcgggt gatcgaagag ctagatgacg
      541 tggacggcga gagcatcgtc ttcgcctacg agccgatctg ggctatagga accggcgaca
      601 ccgcgacgcc cgaggatgcc caggacgcta taggaaagat ccgtgaccta ctcaggcagg
      661 aggtcgggga cgacttcgcc gacgccgtgc gcatactcta cggcggctcc gtgaagccgg
      721 gcaacgccgc ggagataatg gcccaggatg acgtcgacgg cggcctggtc ggcggggcca
      781 gcctcgaggt cgactcgttc gttgagctta tagaagccgc ccagaacgcc gtggccgaga
      841 cagaacaata gagcgtgtgg taaggtagac agatgttact ttactacctg atcgcgttct
      901 tccagatagt gttctgcgtg gcgctggtgg ggctgatcct gctccactcg ggcaagggag
      961 gcggactgtc ctcttcgctc ggcggcggca tgggcagcac cttctctggc accaccataa
     1021 tggagaagaa cctgacccgc ctgaccatca tagttctggg cctcttcgcc ctgaccaaca
     1081 tcgccctgtt cttcctcgcc tgacggccgg tcagcgaatt ctacccataa aagaggactc
     1141 gcccggggag gatggggaga acaggcgagt ccaatctaga ttatacaccc cggtgtgttt
     1201 tcgggcctgc gcgacgtttc acgttccggg gtaatagtgt cggagggggg actcgaaccc
     1261 ccaccccctt atcggggact aggccctcaa cctagcgcgt ctaccaattc cgccactccg
     1321 acaaagcagc ccgcagagta tacgagagac atatagtctg gtcaacgcgg caggccggta
     1381 tcccgctatg ttaagtttcc ccgatgggaa ggaatcggtt tctgctggtc gcgctcgccg
     1441 cgctctcctt gatcgttgct ctgtgcctcc ggcgtgggcg gcggagcatg atcgcgcgcg
     1501 attggcccgt gaacctggcc cacaggggcg cttctgccag cgcgccggag aacacgctcg
     1561 aggcttttcg gctggccgag cgttcgggcg ccggtgggat cgagcttgac gtccatctga
     1621 cccatgatgg gtaccctgtg gtgatccacg acccgaccct ggacaggacc acgaacctga
     1681 ccggcgccgt cgccgagatg gggctcgacg aggtacgcgg ggccgatgcc gggtactgtt
     1741 tcagcaccga cggcggaaaa acctatccct
//
LOCUS       DBNGVD010000335         4878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_259647, whole genome shotgun sequence.
ACCESSION   DBNGVD010000335 DBNGVD010000000
VERSION     DBNGVD010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_259647"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC16CM_08955"
     CDS             <1..418
                     /locus_tag="AC16CM_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3140000.1"
                     /translation="TSADLNGDGRPDLATANGGSSFGASNVSVLLNTTELTETARCTI
                     TGTGRDDVLEGTGGRDIICGRGGDDRIKGSQGDDEILGGRGDDAINGGDGNDVLRGGR
                     GEDRLVGAGGSDALFGGPGVDRCASGNDRETGCEKP"
     gene            complement(481..1350)
                     /locus_tag="AC16CM_08960"
     CDS             complement(481..1350)
                     /locus_tag="AC16CM_08960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140001.1"
                     /translation="MMPRHLLATLSALLLGYLGVGLLVILWMTGPKRKAPWATPASVG
                     LGYEHAEVSSTDGIRLRSWWVPAGDSGRAVVLVPGWGGYKFEDHLLQTLLVYHDAGYG
                     VLMLDLRAQGESKGRRRTLGYREVRDVRGALAWLRSRGYAPGETVLHGWSMGGATVLR
                     AAPGSGVAAVVEEAGYGDLPLLLRRKLPEFVRFGRLLEPAILLLGKLYPDFDYQGVMP
                     KKEAALLSEGGVPLLIIHSVDDGVVPYEQAKLLAAAYPDAKVWTLESYGHVEAYEHPE
                     YAQRLQGFLSSIR"
     gene            complement(1369..3741)
                     /locus_tag="AC16CM_08965"
     CDS             complement(1369..3741)
                     /locus_tag="AC16CM_08965"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR3140002.1"
                     /translation="MGELLDHDDNSLRLRAGSTTVEITALAPDLFRVGMFPAGGPPSY
                     DSEAIARKNWDPVEVSMQESDEELTLSTTAASARISLSPLRIGFSDGSGRKFAVDDPE
                     LGMGAVETAGADIFSEPLGSPVRLYKRRETGERYFGCGERTSGLEKTGSHQIFYNVDP
                     PLGHTASFNNLYSSIPFTFSLVDGKAHGLFFDNTHRVEFDLALADEDRAYYGAEGGAV
                     VYYVFCGPTPADVVDRYTELTGRTPMPPLWSLGNQQCRYSYMNEDQVREVASGFRERD
                     IPCDVIYLDIHYMDGYRVFTWNEDRFPDPKGLISDLAEQGFRVVTIVDPGVKVDENYS
                     VYTEGREKDLYCKTRDGEEYHNAVWPGVCAFPDFTNPETREWWGQNQKVLTDPGVSGI
                     WCDMNEPALFIPDGATMPDNVVHPGGHKGEARWHAQIHNTYGSLMARAAREGLLALRP
                     DERPFVITRAGYAGLQRHAMQWTGDNSSWWEHLWMSLPQLQNLALSGVAWAGVDIGGF
                     GGDTNAELLARWYEFGVFQPYCRNHTTLGTRPQEPWAFGEPYESVCREMIKLRQRLLP
                     YLYTLFEGCHRTGAPILRPLLFEYPEDESTYSADDEFLLGDALLVAPITRPGVEHRHV
                     YLPEGTWFHYYSGERLDGPAHILAHAPLGEPALYLKANTAIPMGPDASHTAEKPTDPL
                     TLLLHPAEGTGESTLYEDAGNGFGYRDGEYARRSVSCESSEGRVLVQLGEREGSFVPG
                     RQEVRLELRGVTAQSVLVDGGEREPEQGEDGPLIVSLDEKAGPTTIEVVL"
     gene            complement(3934..>4878)
                     /locus_tag="AC16CM_08970"
     CDS             complement(3934..>4878)
                     /locus_tag="AC16CM_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140003.1"
                     /translation="LVSRFGSRRIVGLSALALSVSLLTPGLAPSLPLLVLAVMLVGAA
                     NGGLDVAMNAQAVAVERGYGRSIMSSFHAAWSFGGLGGAALGGLLASRGIDPFPHFTA
                     VAILTAIAFAFAYGSLLPSSADAAEEGTPAFVRPSRALLGLGIISFCVLLGEGAMGDW
                     SAVYLDDTLRTGPGFAAAGYAAFSLSMAFGRLFGDRFTELLGPVALVRACGIIAAVGL
                     GVALAAANPIVALVGFACAGAGFSIVFPTALSAAGRSGGSTGPAIAAVTTAAYTGFLV
                     GPPFIGFMAELTGLGYALYLVVALSTAVIILAGAVNTK"
BASE COUNT          850 a         1562 c         1660 g          806 t
ORIGIN      
        1 aacgagtgcc gacctcaacg gagacggcag gcccgacctc gccacggcaa acggcggctc
       61 aagcttcgga gccagcaacg tctctgtgct gctcaatacg accgaattga ccgagaccgc
      121 ccgctgcacc atcacgggga cgggacgcga tgacgttctc gaaggcaccg gggggcggga
      181 catcatctgc ggtaggggtg gtgacgaccg gatcaagggc agccagggcg acgacgagat
      241 actgggtggc cgtggcgacg atgccatcaa cggtggtgac ggcaacgatg ttctaagggg
      301 cggcaggggc gaagaccggc tggtcggcgc aggaggctcg gatgccctgt tcggcgggcc
      361 gggtgtggac cggtgcgcca gtggcaacga ccgcgagacg ggctgcgaga agccgtagca
      421 ggcggcgcaa ccgagacata ggtggcgcgc catctgacgg ggggcctggt ccaatcatct
      481 ctaccgtatc gagctcagaa acccctgtaa cctctgcgcg tactccggat gctcgtaagc
      541 ctcgacatga ccgtagctct cgagtgtcca caccttcgca tccgggtagg ccgcggcgag
      601 cagcttggcc tgttcgtagg ggactacgcc gtcgtcgacg gagtggatga taaggagggg
      661 cacgcccccc tcggagagca acgcggcctc cttcttcggc atcaccccct ggtagtcgaa
      721 atcggggtag agcttgccta gcagcaggat cgctggctcg agcagccggc cgaagcgtac
      781 gaactccggc agctttctcc ggaggaggag ggggaggtcg ccgtatccgg cctcctcaac
      841 gaccgcggcg acacccgatc caggggcggc gcgcagaacc gtcgcgcctc ccatcgacca
      901 gccgtggaga acggtctcgc ccggtgcgta accccggctg cgcagccagg cgagcgcgcc
      961 ccgcacgtcg cgcacctccc tgtagccaag ggtgcggcgc ctccctttcg attcgccctg
     1021 cgccctgagg tccagcatca gcacgccgta gccggcgtcg tggtatacga gaagggtctg
     1081 tagtagatga tcctcgaact tgtagccgcc ccagccggga acgaggacca cggccctgcc
     1141 cgaatccccc gcgggaaccc accaggatct gagccggatc ccatcggtgc tcgagacctc
     1201 ggcgtgttcg tagccgagcc cgacggaggc tggcgtcgcc cacggggctt tacgcttcgg
     1261 gccggtcatc cacaggatga cgagcagccc gacgcccagg tatcccagca gcagggcgga
     1321 cagggtagct agcaggtgac gcggcatcat tcccccggta ctcggatgct agaggaccac
     1381 ctcgatggtg gtgggaccgg ccttctcgtc gagcgagacg atgagcgggc cgtcctcccc
     1441 ctgctcgggc tcgcgctcac caccgtctac gagaacgctc tgcgccgtaa caccgcggag
     1501 ctccaggcgt acttcctggc gccctggcac gaaggagccc tcgcgctcgc cgagttgcac
     1561 gaggacgcgc ccttccgagg actcgcagga aacactccgc ctggcgtact cgccgtccct
     1621 gtatccgaag ccgtttcctg cgtcttcgta gagcgtggat tctcccgtgc cctcggccgg
     1681 atggaggagt agtgtcaggg ggtcggtagg cttctcggcg gtgtgggaag cgtcgggtcc
     1741 catcggtatc gcggtgtttg ctttcaggta cagggctggc tctcccaagg gggcgtgcgc
     1801 caggatgtgc gcggggccat ccaggcgttc accgctgtag tagtggaacc aggtgccctc
     1861 cggcaggtac acgtgacggt gttcgacgcc ggggcgggtt atcggggcga cgagcagagc
     1921 gtcgccgagg aggaactcgt cgtcggcgct gtaggtcgac tcgtcttccg ggtactcgaa
     1981 gaggaggggg cgaagaatcg gggcccccgt cctgtggcaa ccttcgaaga gcgtgtacag
     2041 gtaaggcagc aatcgctggc gcagtttgat catctcgcgg cacacggact cgtagggctc
     2101 tccgaaggcc cagggctcct gtgggcgggt gcccagggtg gtgtggttgc ggcagtaggg
     2161 ttggaagacc ccgaactcgt accagcgagc cagaagctcc gcgttggtgt cgcccccgaa
     2221 gcctcctatg tccacgccgg cccacgccac gccggagagc gcgaggttct ggagctgggg
     2281 caggctcatc cacaggtgct cccaccagga ggagttgtcc cccgtccact gcatggcgtg
     2341 gcgctgcagc ccggcgtagc cggcgcgggt gataacgaag gggcgctcat ctgggcgcag
     2401 ggccagcagg ccctccctcg cggcgcgggc catcagggag ccgtaggtgt tgtggatctg
     2461 ggcgtgccac cttgcctcgc ccttgtgacc gccggggtgg accacgttgt ctggcatggt
     2521 ggcaccatca gggatgaaga gggctggctc gttcatgtcg caccagatgc cggatacccc
     2581 cgggtcggtg aggaccttct ggttctgacc ccaccactcg cgcgtctccg ggttggtgaa
     2641 atctgggaag gcgcatacgc cgggccagac ggcgttatgg tactcttcgc cgtcccgtgt
     2701 cttgcagtac aggtctttct cccggccctc ggtgtagaca gagtagttct cgtccacctt
     2761 gacgccgggg tccacgatgg tgacgacgcg gaagccttgc tccgcgaggt cggagatgag
     2821 gcccttcggg tcggggaagc ggtcctcgtt ccaggtgaag accctgtagc catccatgta
     2881 gtggatgtcg aggtagatca cgtcgcaggg gatgtcacgc tccctgaaac cgctggcgac
     2941 ctcacgcacc tggtcttcgt tcatgtaaga gtaacggcac tgctggttgc ccagagacca
     3001 gagcggtggc atgggggtac gccccgtgag ctcggtatac cggtccacga cgtcggccgg
     3061 ggtcgggccg cagaagacgt agtagacgac cgcgccaccc tcggccccgt agtaggcccg
     3121 gtcctcgtcc gcgagcgcga ggtcgaactc gaccctgtgc gtgttgtcga agaagagccc
     3181 gtgggccttg ccgtcgacga gcgagaaggt gaagggtatg gaactgtata ggttgttgaa
     3241 ggacgcggta tggcccagag gtggatccac gttgtagaag atctggtgtg atcccgtctt
     3301 ctccagcccg ctggtccgct caccgcagcc gaagtacctc tctcccgtct cccgccgctt
     3361 gtacagccgc accgggcttc ctaggggctc cgagaatatg tcggcgcccg cggtctccac
     3421 ggcgcccatc ccgagctccg ggtcgtcaac cgcgaacttc cggcccgagc cgtccgagaa
     3481 cccgatgcgc agagggctca gcgagattcg tgcgctcgcc gcggtggtcg agagcgttag
     3541 ctcctcgtcc gactcttgca tcgagacctc gactggatcc cagttcttcc tggctatggc
     3601 ttcggagtcg taactcggcg gaccgccggc tgggaacatg ccgacgcgga agaggtcggg
     3661 ggcgagggcc gtgatctcca cagtggtaga gccggccctc agccgcaggc tgttgtcgtc
     3721 gtgatccaac aactcaccca cgcctagagg tctatatctt ccaaccgtct cgggctgctt
     3781 ttcaaaggtc tcggacaatg ttcctcccta cggtcgtcac agcaccctac agcccttcgg
     3841 gcccgcgctt gcctgcacgc tcgggctttt acgcccttcg cttgtcggca cggcgcttcg
     3901 caccttttcg gcctactggt tgagggtggc attctacttg gtgtttacgg ctccggccag
     3961 gattataacg gcggtgctga gggctactac gaggtagagt gcgtagccga gccctgttag
     4021 ttcggccatg aagccgatga atggcgggcc gaccaggaag cctgtgtagg ccgcggtggt
     4081 gacggcggcg atggcgggac ctgtggagcc cccgctgcgt cccgcggcgc tcagggccgt
     4141 agggaagact atggagaacc cagccccggc acaggcgaac ccgacgaggg ccacgatcgg
     4201 gttggcggcg gccagcgcga cccccagccc tacggcagca atgatcccac aggcgcggac
     4261 aagcgcgacg gggccgagca actccgtgaa cctgtccccg aagagacggc cgaaggccat
     4321 ggacaatgag aacgcggcgt atccggcggc agcgaagccc gggcccgtcc gcaaggtgtc
     4381 gtccaggtag acggcgctcc agtctcccat agcgccctcc ccgagaagca cacagaatga
     4441 gatgatgccg agcccaagca gcgcccgcga cgggcggacg aatgcgggtg ttccctcctc
     4501 cgcggcatcc gcgctggaag gcaagagaga cccgtaagca aaggcaaagg ctatggcggt
     4561 caggatggcg acagccgtga agtgggggaa ggggtcgatg ccgcgggatg ccagcagacc
     4621 gccgagggct gccccgccga ggcccccgaa gctccaagcg gcgtgaaagg aggacatgat
     4681 cgatcgccca tacccacgct ctactgctac ggcctgggcg ttcatggcca cgtcgagccc
     4741 gccgttggcc gcccccacca gcatgaccgc gagcacgagc aaaggcaggc tcggggccaa
     4801 ccccggcgtc aggagcgaga ccgagagcgc gagcgcgctc aatccgacga tgcgcctgct
     4861 accgaacctg gataccaa
//
LOCUS       DBNGVD010000336         9690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4996801, whole genome shotgun sequence.
ACCESSION   DBNGVD010000336 DBNGVD010000000
VERSION     DBNGVD010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4996801"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16CM_08975"
     CDS             <1..320
                     /locus_tag="AC16CM_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sulfur cluster biosynthetic enzyme"
                     /protein_id="HIR3140004.1"
                     /translation="LCRMLLADGYTAAELAGMRLEDVPDSEAKEKYLSRREELGLDVS
                     GEATFVVDPDGRRVPEEAVIKHLRFARVTRLSIEANAGFCRGVLAARYKDAAWVDKEE
                     ATL"
     gene            317..1378
                     /locus_tag="AC16CM_08980"
     CDS             317..1378
                     /locus_tag="AC16CM_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3140005.1"
                     /translation="MKAARLHRYDDSIPQDSLKVEEIDEPKIEGPLDVIVRIGAAGLC
                     RTDIHVVEGQWQPIQDVDNTLLPYVLGHENSGWVEEVGSAVTNVEVGDTVICHPLMTC
                     GLCHACRAGDDMHCEVGEFPGLSRDGGFADLMKTNARAVVKLDPILEPKDIAALADAG
                     LTAYHAVRKSVPLLYAGTNAVVIGAGGLGHIGIQCLKALTPTRIIVTDPSEEALELAK
                     EWGADETVQVQDGYVDTVQEMTNGGAEVVFDYVGERGAQDDAWEMTRPGGSHYAIGYG
                     GMVEVPTIDIVSTERSITGNLVGTYNDLVELMVLTAQGKVNLHTATYPLDAVNDAMQD
                     LNSGNLRGRGILIPEGATS"
     gene            complement(1520..2506)
                     /locus_tag="AC16CM_08985"
     CDS             complement(1520..2506)
                     /locus_tag="AC16CM_08985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3140006.1"
                     /translation="MLPRAIDNPNAPDNICDVAGVLVGHATDEEGTTGCTVVLFEKPE
                     GTVVGVDVRGSSPGTRETDRLGPSGAVRGTHALLLTGGSAFGLAAADGVVRFLEEKGV
                     GLDVGVARIPLVSAAVLFDVHFGDPGVRPDARMGYRAASSATGGDFAQGSVGAGAGAT
                     VGKVLGPDRAMKGGVGSASVRLDGGLVVGALAAVNAFGEVRNPWTREPIAGPRLDDGT
                     LGDTVELLPQVADRLRWGENTTLGIVATNARLTKPQANKVAQMAHDGLARAIYPVHTT
                     VDGDIVFAAGTGETEAVPDLVGAWGARVMEDAIVRAVLAARGSKGIPAARDL"
     gene            complement(2619..3395)
                     /gene="ricT"
                     /locus_tag="AC16CM_08990"
     CDS             complement(2619..3395)
                     /gene="ricT"
                     /locus_tag="AC16CM_08990"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HIR3140007.1"
                     /translation="MVVATEVGDLVGRVALTPRRREPYTEPYDIVRVVTPEDRRTEDR
                     NRELGREIREEARKLARDYRIEGIEFIGCDVSLDGTYVEVKYQHDKEVDLQPVKRGLE
                     SSYDVQVARREFRFIERSADTGGCDTCGLPLCCATWSGARNLGPVNVRLAKQQGVTPN
                     EKIIGCCGEVKCCMRFEHETYKEFKERAPFRNSTVKLDDREGKVVDYSMVKDAVMVQF
                     GTRRSDQQLVTLGTLAKDNPGIMPADTDEWEVADPSSADA"
     gene            complement(3443..4555)
                     /locus_tag="AC16CM_08995"
     CDS             complement(3443..4555)
                     /locus_tag="AC16CM_08995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR3140008.1"
                     /translation="MAEARDRFEGVLGNETARRLLGRALRTGEVSQAYLFHGPPGVGK
                     RTVAVRFGAALVGGGDPAAEDRALRGLHPDLTEVKPEGAFTTIGQIRDVLRLAASRPF
                     EGARRVFVLQADSLNVQAANALLKTLEEPEGETVFVLLATSREGVLPTILSRAQAVRF
                     NPVPTDVVAEFLAKRGAREPELAAALGRGSVGLSLRYAEDPEFGELREAVFRAGFSFS
                     EDYERRHEAAAEIVGRAEAVGAAREREVLDRFEEPDRRAKDGAKRAARGARDGALRES
                     LELLALLYRDAAVVEAGAEELVANMDRVAEIRERVDRYPRADWAGAALALGEARAALA
                     YNVSPEATLEVTLSSIRRKILVRSPGSSTSGSLAGA"
     gene            complement(4548..5162)
                     /gene="tmk"
                     /locus_tag="AC16CM_09000"
     CDS             complement(4548..5162)
                     /gene="tmk"
                     /locus_tag="AC16CM_09000"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3140009.1"
                     /translation="MEGPDFSGKSTLLRAIRPLLVEANTVTHFTREPGGTPVAERIRD
                     LVLDAAVEMDAWTEAYLYAAARADHVRNGILPRLLAGQNVLCDRYLDSSIAYQGVGRG
                     LGVEEVLALNAYAVGTLVPDRTFYLRLGSRERERRARALGAPDRIEAVGAEFMRRVEE
                     GFEQLVRLEPERVEVLDAALAPEVLAQLVVGRIGELQYTRGSDG"
     gene            complement(5217..5981)
                     /locus_tag="AC16CM_09005"
     CDS             complement(5217..5981)
                     /locus_tag="AC16CM_09005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3140010.1"
                     /translation="MAPIIGITGTLKEDVEVTAERPLGKYVRTDLDYVAGVAAAGGAP
                     MVLPPLGEERFAASVVGCLDGLLLSGGSDLDPRYYGEEPDPELGPTIPERDAFEMALV
                     ELALRRGIPIFGICRGMQVLNVALGGTLYQDLPSQWGGDVLKHRQVTPKWQPSHEAEV
                     QDGTYIAEVMGRGVVKVNSYHHQGVKDLAEGLAVTARSEDGVVEAVESRDLSERWLLG
                     VQWHAEAMRGTGPQQERLFEAHVSAAERHAVRKAAA"
     gene            complement(6150..8258)
                     /locus_tag="AC16CM_09010"
     CDS             complement(6150..8258)
                     /locus_tag="AC16CM_09010"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HIR3140011.1"
                     /translation="MRLIISEKANTAKKIAQFLSEGPVKEGKHRSVPHHTFTWDGEET
                     VSVGLKGHVLNPEYPEEYSNWQKVEPKELIDAEILKSVSEKGVAQAVTSLAKKADSVI
                     IATDFDREGELIGVEALSLVFEANPKLTDHVRRARFSALTPGEVGRAFDELLEVSNDL
                     ADAGEARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLVLIADRERERRAFI
                     PEPYWELEVNLKNGDPFSVRHQTERFKDEARAKSAYENISDTATVEEIQQKSATRKVP
                     TPFNTTAFLTAASNIGFSPSRAARLAEDLYTDGYISYPRTDNTVYPRSLDLREVLEYL
                     RNVEGVGQHVEKLLKQEELSPTRGKKETTDHPPIYPTGHATRGAMRDDQWRIYQLVVR
                     RFLATLSKQAKTLRTTLRFDSGGETLVTGGTVVTEEGWLGVYPYSRRADEELPNLSEG
                     EVVQVIGKEIHAKETQPPGRYGAGRLINVMEDLGLGTKATRPNIIQNLYDRGYVHDDP
                     LIPTEKGMSVAQALTDFASEIASHEMTAELERSMDEISEGKISKDTVVDSSREVLRKV
                     YDHLQSSETEFADIVWEGIRTDETLGKCPESGHDLIVRRNRKSGKRFVGCKGYPDCRV
                     TYPLPQRGTIIPLGTQCDACGSPEIKVLGGKRPWVTCINMDCPKKQEQREAAEKAKAE
                     QEGGEGSSTQSAQELEKSTT"
     gene            complement(8339..8938)
                     /locus_tag="AC16CM_09015"
     CDS             complement(8339..8938)
                     /locus_tag="AC16CM_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3140012.1"
                     /translation="MDRLLVGVSGSVAVLNLPAYLVTLRAELASQVRVIMTRQAARML
                     PPSTVALICDEVFLDQEPTTVKKPGHIELARWADMFVILPATANVIGQAANGLGSNLL
                     TTTILAATGPVVFCPNAHPDMWNKPVVRRNVETLRQDGHAVIEPEVAAAYEVDSGEMR
                     ESLVLPEPSVLARQLGEIHRDHGPDTTPRSDPLSRPTGR"
     gene            complement(8967..>9690)
                     /locus_tag="AC16CM_09020"
     CDS             complement(8967..>9690)
                     /locus_tag="AC16CM_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140013.1"
                     /translation="ELAVAAKLEYARNRWLDELADKPSYAYGLSAEHRLNEALLGLIR
                     SRYSRVLDGPVATAFFSVLANLYACYGLSLALDSTWCRNPTSALTLQDYAEHARMRHG
                     PVRAPIDAVLMLVGAEEELLQRARSSWHNWALGVQFYDDALDIEEDFRDRNLSWTVAR
                     ALEHHEGYSDTPSSGERDAFYEMALKEGVICEALGHAGSFFDEAARLAEPEFPGWVAY
                     QRACESRVENLRVDYEKLLAGA"
BASE COUNT         1596 a         3239 c         3085 g         1770 t
ORIGIN      
        1 aactctgcag gatgctgctc gcggacgggt acaccgcggc ggagctcgcc gggatgcggc
       61 ttgaggacgt tccggactcg gaggcgaaag agaaatacct cagccgcagg gaggagctcg
      121 ggctcgacgt ctcgggggaa gccacgttcg tggttgatcc cgacggcagg cgggtacccg
      181 aagaggcggt catcaagcac ctgcggttcg ccagggtgac ccgtctaagc atcgaagcga
      241 acgcaggctt ttgcagggga gttctggccg cacgctacaa ggacgcggcc tgggtcgaca
      301 aggaggaggc gacgttatga aggccgcgag gttgcaccgt tacgatgaca gcattccgca
      361 ggacagcctc aaggtcgaag agatcgacga accgaagata gagggtccgc tcgatgtgat
      421 cgtgcgtatc ggggcggcgg ggctgtgccg cacggacata cacgtcgtcg agggtcaatg
      481 gcagcccatt caggacgtgg acaacacgct gcttccctac gtgctcggcc acgagaactc
      541 cggctgggtc gaagaggtcg gctctgcggt aaccaacgtg gaggtcgggg acacggtgat
      601 ctgccacccg ctcatgacct gcgggctgtg ccacgcctgc cgcgccgggg atgatatgca
      661 ctgcgaggtc ggcgagttcc ctggtctctc ccgcgacggc ggcttcgccg acctcatgaa
      721 gaccaacgcc cgcgcggtcg tcaagctcga ccctatcctt gagccaaaag acatcgcggc
      781 gctcgcggat gccggcctca ctgcctacca cgccgtacgg aagtccgtcc cgctcctcta
      841 cgccggtacg aacgccgtgg tgatcggggc cgggggactc ggacatatag gcatccagtg
      901 cctaaaggcg ctgaccccga cgcggatcat cgtcaccgac ccctcggagg aggcgctcga
      961 actcgccaaa gagtggggag cggacgagac ggtacaggtg caggatggct atgtcgacac
     1021 cgttcaggag atgacgaacg gcggcgccga ggtcgtcttc gactacgttg gcgagcgggg
     1081 cgcgcaggac gacgcctggg agatgacgcg cccaggtggc tcacactacg ccataggcta
     1141 cggcggcatg gtcgaggtgc cgaccataga catcgtctcg accgagagaa gcatcaccgg
     1201 caacctggtc ggcacctaca acgacctcgt agaactcatg gtgctaaccg cccagggcaa
     1261 ggtcaacctg cacaccgcga cctacccgct cgacgccgtg aacgacgcca tgcaggacct
     1321 caactcaggc aacctgcgcg gaaggggaat cttgatcccg gagggcgcaa cgagttagag
     1381 agctttcagc tatcagcctt cagcagtcag ctttttggct tgctgaaggc cggtagcgat
     1441 ctgcgcgaag agttgattga aggagggcta cagaggtttt cttcctgaag cctgaaacct
     1501 gtgtcctgta gccttcaagt catagatccc ttgcggcggg gatacctttc gagcccctgg
     1561 cggctagcac ggcgcgcact atagcgtctt ccatcacgcg cgccccccag gcacctacga
     1621 gatccggcac ggcttccgtc tcgcccgtac cggcggcaaa gacgatgtcg ccgtctacgg
     1681 tggtatgcac ggggtagatg gcccgggcca ggccgtcgtg ggccatctgg gccaccttgt
     1741 tagcctgggg cttggtcagg cgggcgttgg tagcaactat cccgagggtg gtgttctccc
     1801 cccacctcag ccgatccgcg acctgcggga gtagttccac cgtgtcgccg agcgtgccgt
     1861 cgtcgagtcg ggggccggcg ataggctcgc gggtccacgg atttctgacc tctccgaagg
     1921 cgttgacggc tgcgagggcg cctacgacga ggccgccgtc gagcctgacc gaggcgctcc
     1981 ccacgccgcc cttcatggcg cggtccggcc cgagaacctt gccgaccgtc gcccccgcac
     2041 cggcacccac gctgccctgt gcgaagtcgc cgcccgtggc cgaggaggcg gccctgtaac
     2101 ccatccgggc gtcgggtcgt accccggggt cgccgaagtg gacgtcgaag aggacggccg
     2161 ccgagacgag aggtatgcgg gccaccccga cgtcgagtcc cacccccttc tcttctagaa
     2221 aacggacgac accgtccgcg gcggcgagcc cgaacgcgct tccaccggtc agtaggagtg
     2281 cgtgcgtccc acgcacggcg ccgcttggtc cgaggcggtc ggtctcgcgg gtgcccggcg
     2341 aggagccgcg cacgtccacg ccgacgacgg tcccctccgg cttctcgaag agcacgacgg
     2401 tgcagcccgt tgtgccctcc tcgtcggtgg cgtggccgac gaggacgccg gccacgtcgc
     2461 agatgttgtc tggcgcgttg gggttgtcga tggccctggg gagaataaac cgctcctaga
     2521 ggggaggttt caggtaacag gtttcaggca tcagttattc ccttgctgac gcccgttgcc
     2581 tgttgccacc gtaggttcac tctttccgct ttgctgcttc aggcgtccgc ggaagaggga
     2641 tccgcgacct cccactcgtc cgtgtcagcc ggcattatgc cggggttatc ctttgcgagg
     2701 gtaccgagcg ttaccagttg ctgatccgag cgcctcgtgc cgaactggac catgacggcg
     2761 tccttgacca tggagtagtc gacgacctta ccctccctgt cgtccagttt cacggtcgag
     2821 ttgcggaacg gcgcgcgctc cttgaactcc ttgtaggtct cgtgctcgaa gcgcatgcag
     2881 cacttcacct cgccgcagca gccgatgatc ttctcgttcg gcgtcacgcc ctgctgcttg
     2941 gcgagcctga cgttgacggg ccccaggttt cttgccccgc tccaggtcgc gcagcaaagg
     3001 ggcaacccac aggtatcaca cccgcctgta tccgcggacc gctcgatgaa tcggaactcc
     3061 cgccgggcga cctggacgtc gtaggaactc tcgaggccac gctttacggg ctgaaggtcg
     3121 acctctttat cgtgctggta cttgacctcg acgtaggtgc catcaaggga gacgtcgcag
     3181 ccaataaact cgatgccttc gatcctgtag tcccgcgcaa gcttccttgc ctcttccctt
     3241 atctcccggc cgagctccct gttgcggtcc tcggtcctcc ggtcttcggg ggtgacgacc
     3301 cttacgatat cgtagggctc ggtgtatggc tcgcgccgcc gcggtgtgag ggcgacgcgc
     3361 cccaccagat cccccacctc ggtcgcgacg accaccctat agccgacccg caggtccagg
     3421 tcccgcgcgt agcagagcct ggttacgctc cagccaggga ccctgacgtc gacgagcctg
     3481 gggagcgtac gagtatcttc cgccgtatgc tcgataacgt cacctccagg gtcgcctccg
     3541 gggagacatt atacgcaagt gccgcccgcg cttcccccag ggcgagcgcc gccccggccc
     3601 aatcggcccg gggataccga tctacgcgct ccctgatctc cgcgaccctg tccatgttgg
     3661 caacgagttc ctccgcgccg gcctcgacaa ccgcagcgtc cctgtacagc aacgccaaca
     3721 actccaggga ctcgcgcaga gcgccgtcgc gcgcccccct ggcggcccgt ttggccccgt
     3781 ctttcgcccg ccggtcgggt tcctcgaaac ggtcgagcac ctcgcgttcg cgcgcagccc
     3841 ctacagcctc tgcccggccg acgatctccg cggcggcctc gtgccttcgc tcgtagtcct
     3901 ccgagaagga gaaacctgcc cggaagaccg cctcccgcag ctcgccgaac tccgggtcct
     3961 cagcgtagcg gagcgagagc cccacgctcc ccctcccgag tgccgcggcg agctcgggct
     4021 ccctggcgcc ccttttcgcg agaaactcgg ccacgacgtc cgtcggtacg gggttgaaac
     4081 gcacggcctg ggcacgggaa aggatggtgg gtagcacccc ctcgcgcgag gtcgcaagca
     4141 gcacgaaaac cgtctcgccc tcaggctcct cgagcgtctt caggagcgcg tttgcggcct
     4201 gcacgttgag gctgtcggcc tgcaagacga acaccctgcg ggcgccctcg aaaggccggc
     4261 tcgccgcgag acgaagaacg tccctgatct ggccgatggt cgtaaaagct ccttcgggct
     4321 tgacctccgt caggtcggga tgtagcccac gcagggcccg gtcttcagcc gcagggtctc
     4381 cccccccgac cagtgccgcc ccgaacctca cggccaccgt ccgcttgccc acgccgggcg
     4441 gaccatgaaa caggtaggcc tgggagacct ccccggtccg taatgccctc ccgagcaacc
     4501 gtcgtgccgt ctcgttcccg agtacgccct cgaatctgtc ccgcgcctca gccatcgctg
     4561 ccgcgagtat attggagttc accgatccta cccaccacca gttgggccag cacctcgggc
     4621 gccagcgctg catcgagcac ctcgacccgc tccggctcca gccggacgag ttgctcgaag
     4681 ccctcctcga cccgccgcat aaattcggcg ccgacggcct cgatcctgtc aggagccccg
     4741 agcgccctgg cccgccgctc gcgctccctg gacccgagcc tcaggtagaa ggtcctgtcc
     4801 ggtacgaggg taccgacggc gtaagcgttc agggccagca cctcctccac accaaggccc
     4861 ctccccacgc cctggtaggc gatgctcgag tccaggtacc ggtcgcagag cacgttctgg
     4921 ccagccagaa ggcgcggcag gatgccgttc ctgacatggt cggcgcgggc agccgcgtac
     4981 aggtaggcct ctgtccacgc gtccatctcc acggccgcgt ccaggacgag gtccctgatc
     5041 cgctccgcca cgggcgtgcc ccccggctcg cgcgtaaagt gggttacggt gttggcctcc
     5101 acaagaaggg gcctgatggc acgcagaagg gtggatttgc ccgagaaatc cgggccctcg
     5161 atggttacga agatgcccct gcgtgtgccc ggcgggttct gcgcggcaat ctttctctag
     5221 gccgcggcct tgcgcacggc gtgacgctcc gccgccgaga cgtgcgcctc gaagagccgc
     5281 tcctgctgcg ggccggtacc gcgcatggcc tccgcgtgcc actgtactcc gagcagccag
     5341 cgctcggaca ggtctctgga ctccacggcc tcaaccacac cgtcttccga gcgggccgtc
     5401 accgcaagcc cctctgcgag gtctttgacc ccctggtggt ggtaggagtt gaccttgacc
     5461 acaccccggc ccatgacctc agcgatgtac gtcccgtcct gaacctccgc ctcgtgcgag
     5521 ggctgccact tgggggtgac ctgcctgtgc ttgagcacgt cccctcccca ctgtgagggc
     5581 aggtcctggt acaacgtccc gccgagcgcc acgttgagta cctgcatccc gcggcagatc
     5641 ccgaagatgg ggataccgcg cctcaacgcc agttcgacta gcgccatctc gaaggcgtcg
     5701 cgctcgggga tggtgggccc gagctcagga tccggctcct caccgtagta gcggggatcc
     5761 aggtcgcttc caccgctcaa caacagacca tcgaggcacc ctaccaccga agcagcgaac
     5821 ctctcctccc ccagcggggg caacaccatg ggagccccgc cggccgcggc aacgcccgcg
     5881 acgtagtcca ggtcggtcct tacgtacttg ccgagaggcc gctcggcagt cacctcgacg
     5941 tcttccttca aagtgccggt aatgcctatg atcggggcca agagttacac acccttaaca
     6001 gtatcttcac ttctcttaac tatatacggc agaataccag atcgcgacca ccatagcgag
     6061 cagtatcagg taccctgtgg cacggatggc ccttgagggt tggtgataga agggcggttc
     6121 atcggcgagg tccaggtcca gcctgttctc tatgtggtgg atttctccag ttcctgggcg
     6181 ctctgtgtcg atgagccctc acccccctct tgttctgcct tcgccttctc ggcggcttca
     6241 cgctgctcct gctttttcgg acagtccatg ttgatacacg ttacccaggg acgcttaccc
     6301 ccgagaacct tgatctccgg cgagccgcag gcatcgcact gggtgcccag gggtattatg
     6361 gtgcctcgct gcggcagcgg ataggtcacg cgacaatccg ggtagccctt gcagccgacg
     6421 aaacgcttgc cgctcttgcg gttgcggcgc acgatcaggt cgtgcccgct ctcggggcac
     6481 ttgccgaggg tctcgtcggt ccttattccc tcccacacga tatccgcgaa ctcggtctcg
     6541 gagctctgca ggtggtcgta gaccttgcgt agtacctcgc gtgatgagtc gacgacggtg
     6601 tccttgctga tcttgccctc cgagatctcg tccatgctcc gctccagttc cgcggtcatc
     6661 tcgtgagatg ctatctccga ggcgaagtcc gtaagggcct gggccacact catgcccttc
     6721 tcggtaggga tgagcggatc gtcgtgaacg tagccacggt cgtagaggtt ctggatgatg
     6781 ttcgggcgcg tcgccttggt gccgagcccg aggtcctcca tcacgtttat gaggcggccg
     6841 gcaccgtaac gccccggcgg ctgggtctct ttggcgtgga tctccttgcc tatgacctgc
     6901 accacctcgc cttcggagag gtttggcagc tcctcgtcgg cgcggcggct ataggggtag
     6961 acgccgagcc atccctcctc ggtaacgacc gtgccacccg tcacgagcgt ctcgccgcca
     7021 gagtcgaagc gtagcgtggt gcgaagcgtc ttggcctgct tcgagagcgt ggcgaggaag
     7081 cggcggacta ctagctggta gatcctccac tggtcgtcgc gcatcgcgcc tctggtggcg
     7141 tggccggtcg ggtagatcgg cgggtggtcc gtcgtctcct tcttgccccg cgtgggcgag
     7201 agctcctcct gtttcagcag cttctcgacg tgctggccta cgccctccac gtttctcaag
     7261 tattcgagta cctcgcgcag atccagcgag cgcgggtaga ccgtgttgtc cgtcctgggg
     7321 taggagatgt agccgtccgt gtacaggtcc tcggcgaggc gggccgccct cgaagggctg
     7381 aacccgatgt tcgaggcagc cgtcaggaag gccgtggtgt tgaacggcgt gggcaccttg
     7441 cgggtcgccg acttctgctg gatctcctcg acggtggccg tatcggagat gttctcgtag
     7501 gcgctcttcg cccttgcctc gtccttgaag cgctcggtct ggtggcggac gctgaaggga
     7561 tcgccgttct tcaggttcac ctcgagctcc cagtagggct cggggataaa ggcccggcgc
     7621 tcgcgctcgc ggtccgctat gaggacgaga gtcggggact gcacgcgtcc caccgacagg
     7681 aacgcgctgc cgtatcgttt cgtggcgcgg gacacccaac gggtaagggt ggcgccccag
     7741 atcaggtcaa tatcctgccg cgcctcgccg gcgtcggcca ggtcgtttga gacctccagg
     7801 agctcgtcga aggcgcgtcc gacctcccct ggcgtgaggg ccgaaaagcg ggcgcgacgc
     7861 acgtggtccg tgagcttcgg gttggcctcg aagaccaggg agagggcttc gaccccgatc
     7921 aactccccct cgcggtcgaa gtccgtggcg atgatgacgc tatctgcctt cttggcgagc
     7981 gaggttacgg cctgggccac gcccttctcg gagacggact tgaggatctc ggcgtcgatg
     8041 agctccttgg gctcgacctt ctgccagttg gagtactcct cggggtactc ggggttcagc
     8101 acgtgcccct tgaggccgac ggagaccgtc tcctcgccgt cccacgtgaa ggtgtggtgg
     8161 ggcaccgagc ggtgcttgcc ctccttgacc gggccttcgg agagaaactg ggcaatcttc
     8221 ttggcggtgt tcgccttctc ggagataatt aatcgcatat tgatcctggt tataacacaa
     8281 tgctgcgggt tgccccgtgc gcttccgtga ggaagttctg gcccacgcgg ggaggactct
     8341 agcggccagt gggacgagag agcgggtcgc tgcgcggggt ggtatctggt ccgtggtccc
     8401 ggtggatctc cccaagttga cgcgccagga cggatggttc agggagcacc aggctctctc
     8461 gcatctcgcc ggagtcgacc tcgtaggcgg cggcaacctc cggttcgatc accgcgtgcc
     8521 cgtcctggcg cagggtctcc acgttccgac gtacgacggg tttgttccac atgtcggggt
     8581 gagcgtttgg gcagaagacg accgggcccg tggccgcgag gatcgtggtg gtcaacaggt
     8641 tcgaaccgag gccgttggca gcctggccga tcacgttcgc ggttgccggc aggatcacga
     8701 acatatcggc ccagcgcgcg agctctatgt gtcccggctt ctttacggtc gtgggttcct
     8761 gatccaggaa tacctcgtcg cagatcaggg cgacagtgga agggggcagc atgcgtgccg
     8821 cctggcgggt catgatcaca cgcacctggc tggcgagctc cgcgcgtagg gtgaccaggt
     8881 aggccggaag gttgagaacg gccacggaac ccgatacccc aaccagcaaa cggtccatcg
     8941 ctatctcccc cgtttccgga gccgcttcag gctccggcta gcagcttctc gtaatctacc
     9001 cgcaggttct cgacccggct ctcgcacgcg cgctggtagg ctacccagcc aggaaactcg
     9061 ggctcggcga gccgggcggc ctcatcaaag aacgaccctg catgaccgag ggcttcacag
     9121 atcacaccct ccttcaacgc catctcgtag aaggcatccc tctcgccaga ggacggggtg
     9181 tcggagtacc cttcgtggtg ctccagggca cgcgccacgg tccagctcag gttgcgatcc
     9241 cgaaaatctt cttcgatgtc gagggcatcg tcgtagaact ggacgccgag ggcccagttg
     9301 tgccacgagg acctcgcccg ctgcagaagc tcctcctcgg cccccacgag catgagaacg
     9361 gcatcgatcg gggcgcgtac cgggccgtgg cgcatcctgg catgctcggc gtaatcttgg
     9421 agggtcaagg cggatgtggg gtttcggcac cacgtgctgt cgagcgccag ggacaacccg
     9481 tagcacgcgt agaggttggc gaggacggag aagaatgccg tggcgacggg gccgtcaaga
     9541 acgcgggagt acctggaacg tatgaggccg agcagcgcct cgttcaggcg gtgctccgcc
     9601 gacaatccgt acgcgtagga tggcttgtcg gccaactcgt cgagccaccg attgcgtgcg
     9661 tactcgagct tggccgccac ggccagttct
//
LOCUS       DBNGVD010000337         4051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1353900, whole genome shotgun sequence.
ACCESSION   DBNGVD010000337 DBNGVD010000000
VERSION     DBNGVD010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1353900"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..435
                     /locus_tag="AC16CM_09025"
     CDS             70..435
                     /locus_tag="AC16CM_09025"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3140014.1"
                     /translation="MSAEEMKALERRLIEEVWNEGNLDVAEECFTPDYVNRDPSIPEE
                     IRGLEGFKHNVTAIRSAFPDFRMRIVDQVAEAEKLVTRYVITGTQEGELAGIPPSGRR
                     IEVAGMGIDYFLGSKIFES"
     gene            complement(926..1270)
                     /locus_tag="AC16CM_09030"
     CDS             complement(926..1270)
                     /locus_tag="AC16CM_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140015.1"
                     /translation="MTAGHVPPTSRWRRFISLPTTRVGWYSLGFLGAHGLVMVALGIA
                     SGVLAATGAPNLAGHPWLIGVVLFAALSPAIAGIVMGLIAVIGRGERSILVVGPVLLV
                     VLFLLAEILVPH"
     gene            complement(1625..2449)
                     /locus_tag="AC16CM_09035"
     CDS             complement(1625..2449)
                     /locus_tag="AC16CM_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140016.1"
                     /translation="MEKVRTASFEIEEERVAWEGLASACSGPIRRFGAFVLVGFTSFI
                     VITTAVILGYHLLDDRPAGQGATVPWAVFDLTMLSGVVVAVGGYLIWLLWSLRSYAGF
                     AAVLRRGGLDPGRPTSGGLRAYSDEQLLALRSRFERPREGKLRERLGRTFGFHDADEF
                     SLGPLSVLPGTFEMGALRMEWETQLILRSDEPLPSISWWTEGRHRLLPRRPDDVGKLV
                     YALRYTGDSVRELKRRYGYRTDRWHATVPEGKLFDAVRDLENSHRIQGALSRRLPG"
     gene            2760..3590
                     /locus_tag="AC16CM_09040"
     CDS             2760..3590
                     /locus_tag="AC16CM_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3140017.1"
                     /translation="MKVYRAGRVNDGGGRRGGLLRPALLVLLVLFVLLLIYLLVPIGG
                     QRVVLLGSDARGDEVSRSDTIVVTRAGRGMLAVPRDTLVDIPGVGKDKINAAYASGGP
                     ELTLETLQNLTGLRLKHYVVVNFGGVEEIVDAMGGITLQVDNPINVGIDGRRVSIPAG
                     TQDLNGLQALAYVRYRGGPTADIGRIGRQQKFLRELARQSTSPANLPRLPAVAAATWR
                     NIDTNMNILQAARFAIRTRLSGIQGAELYPGTPQYIDGVSYWVPDKEAGDRVVSETVE
                     "
     gene            complement(3823..>4051)
                     /locus_tag="AC16CM_09045"
     CDS             complement(3823..>4051)
                     /locus_tag="AC16CM_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140018.1"
                     /translation="ALRHFMRALQEDALVGRELSVTTYLVGELNRRLGNHREALNWYV
                     NAGRTTEGDPRAAWLDRLIDQQSKLAREQAA"
BASE COUNT          774 a         1255 c         1307 g          715 t
ORIGIN      
        1 gcagcataag tgatcggccg gacgtgcggc tgcacgcccg accctgcacg gcaggaggga
       61 ggcatcgaca tgtcggctga agagatgaag gctctcgagc gccgtctaat cgaggaggtg
      121 tggaacgagg gcaacctcga cgtcgccgag gagtgcttca cccccgacta cgtcaaccgc
      181 gacccctcga tccccgagga gatacgcggc ctcgaaggat tcaagcacaa cgtcacagcg
      241 attcgctcgg ccttccccga cttccgtatg cggatagtcg atcaggtggc agaggcagag
      301 aagctggtta cgcgctacgt gatcaccggc acccaagagg gggagctggc gggcattccg
      361 ccgagcggca gacggatcga ggtggccggg atgggcatcg actacttcct ggggagcaag
      421 atcttcgagt cgtgagagta ctacgacgtg ctggggctga tgcagcagtt gggcgccatc
      481 ccatttcccg aagagcagcc cggctagggt cggtacacga acgactcacc agggggttca
      541 tcttcccgcc gcctcccggt gcctttattc actgaagtgc gtggaagggc agttctcaga
      601 agtcggactt ccattctacg gagttcaaag gagtctatga ggaaagatat gaaggttctc
      661 gccttttgct agattcatcg acgcttgcta acgtggtggc ctcgtgcggc tcggcgggct
      721 tgtcgaggct ctctgttatg gagggggagg agatgagaaa caccgttctg gttctcgctt
      781 cgatgacact ggctggcctg atggcatgca cggctgccgt gctcacggca gtaccgggca
      841 acgtgtgtgg agcatgcccg gcactcatgg cgacctcctc acttcttctg tgtgccatgc
      901 tcttgctagt agcgcttcat cgctcctagt ggggtacgag gatctccgct aacaggaaca
      961 gaacgacgag tagcacggga ccgaccacca atatcgaacg ctcgccccgt ccgatgaccg
     1021 cgatcagccc catcactatc cccgcgatgg cgggactcaa tgccgcgaac agcaccaccc
     1081 ctatgagcca ggggtgaccg gccaggttcg gcgctcccgt ggcggcaagg acaccactgg
     1141 cgatgcctag cgccaccata acgagcccgt gggcacccaa gaacccgagc gagtaccatc
     1201 cgacgcgggt ggtaggcagc gagataaatc ttctccaccg cgatgtgggg ggaacgtgcc
     1261 cagcagtcat cgcgtgctcc cttcaaaggc cttctctagt gcaacgtgat tgtgccgctt
     1321 cggagagcca ccggcatggc acacatcgag cgttctgctg agcaaccttg gtcaagttct
     1381 cggatcagat ggcactaggg gacctattcg ccggcgtgcg cataaggatg attcttcgaa
     1441 gttcaacgta gcaagcggcc cgtcgagcga ggcggaaagt accctacagc cgcacggcgc
     1501 atcgtcatcg caatctgctg cgaacggtcg aagcgtgccg acaagtgaag ctgaaggccg
     1561 atacctgctg agaagacgcg aagcgccgtg ctgaggagcg gaggcgttag tcggagcgtg
     1621 ctccctagcc gggtaacctg cggcttagcg cgccctggat gcgatgggag ttctcgaggt
     1681 cgcggacggc gtcgaagagc ttgccttccg gcacggtagc gtgccaacgg tcggtcctgt
     1741 agccgtagcg gcgcttgagc tcacgcaccg agtcgccggt gtaacggagg gcgtagacga
     1801 gcttgccgac gtcgtcaggc ctgcgcggca ggagcctgtg acgtccctcg gtccaccacg
     1861 agatgctcgg caacggctcg tcggagcgca ggatcaactg cgtctcccac tccatcctga
     1921 gtgcccccat ctcgaacgtg cctggcagca cgctcagggg tcccagggag aactcgtccg
     1981 cgtcgtggaa tccgaacgta cgcccgaggc gctcccttag cttcccctct ctcggccgct
     2041 cgaagcggga ccgcagggcg agcaactgct cgtcggagta cgcccgcaga ccgcccgacg
     2101 taggacgacc cgggtccaga ccaccccggc gcagcaccgc tgcgaaaccg gcgtacgacc
     2161 gcaggctcca gagcagccag ataaggtagc caccgacggc aacaacgacg cccgagagca
     2221 tcgtcaggtc gaatacggcc catggaaccg tggcgccctg cccggcgggc cggtcatcga
     2281 ggagatggta gcccaggata acggcggtgg tgatcacgat aaaggacgtg aacccaacca
     2341 gcacgaaagc cccgaaccgc cggataggcc cggaacacgc cgaggcaagc ccctcccagg
     2401 ccacacgctc ctcttcgatc tcgaacgaag ctgtacgcac cttttccacg gtcagaatta
     2461 taaccacggc gaacaccccg ggctgggtta gagttcacgc tgaagagcac gctccggctt
     2521 cgcctccgct tgtcagcaca ctacggcctg cggcctcgct tgtcggcaac gcttcggctt
     2581 cgcctcgctt tcggctagtc gtctcggtcc gcatcggtgg cttcgacaac caacctcttc
     2641 ccggcaccgc ttctgtcgtg cggaaggtgc aggcagcaac gggagcgtgc tgacaaggcg
     2701 ccgcaggcgc cgtgctgaca agtggaggcg gaaggccgga gcgtgctaaa ttcataggca
     2761 tgaaggttta cagggccgga agggtgaatg atgggggcgg gcgacggggt ggcctgctaa
     2821 ggccggccct gctggtgctc cttgttctct tcgtgctctt gctcatctat ctgctcgtgc
     2881 ccatcggagg gcagcgggtg gtcttgctcg gcagcgacgc gaggggggat gaggtttcgc
     2941 gctcggacac catcgtagtg accagggcgg ggcggggcat gctcgccgtt ccgcgcgaca
     3001 cgctcgtgga catccccggc gttgggaagg acaagatcaa cgccgcctac gccagcggcg
     3061 ggccggagct gacgctggag acgctccaga acctgaccgg gctgcggctg aaacactacg
     3121 tcgtcgtgaa cttcggcggg gtggaggaga tagtcgatgc catgggtggg atcaccctgc
     3181 aggtggacaa cccgattaac gtcggcatcg acggccgccg cgtctctata ccggctggca
     3241 cccaggacct caacggtctg caggcgctcg cctacgtccg ctacaggggc ggtcccacgg
     3301 cggacatcgg caggataggc cgccagcaga agttcttgag ggagctggcc cggcagtcga
     3361 cctcgccggc caacctgccc cgcctacccg ccgtcgcagc cgcaacctgg cgcaacatcg
     3421 acacgaacat gaacatcctc caggccgccc gcttcgccat ccgcacccgc ctctcaggca
     3481 ttcagggagc ggaactctac cccggcacgc cgcaatacat agacggcgtc tcctactggg
     3541 tgccggacaa ggaggcggga gatagggtcg tgtcggaaac cgtcgagtaa taggtttcag
     3601 gttacaggca tcaggtttca ggtaggtctg tccacgctac gcgggtgaag accacgccgt
     3661 tcgtgttaat gggtggagtt ggtttcgccg tctagcgtgt agcccgttag ataagcgtgc
     3721 tcgtgtaaag cccgtgtctc ttcgtaatag gcgctatcgc gaccgcccaa atgacccgtc
     3781 ttccaaggtc tgaagcctga aacctgcagc ctgaagcctt ctctaggccg cctgttctct
     3841 ggctagcttg ctctgctggt ctatcagcct gtctagccat gcggcgcggg ggtcgccttc
     3901 cgtggtgcgt ccggcgttga cgtaccagtt gagagcctcg cgatggtttc cgaggcggcg
     3961 gttgagctcg ccgacgaggt aggtggtgac ggagagctcg cgtccgacga gggcgtcttc
     4021 ctgcagggcg cgcatgaaat ggcggagggc c
//
LOCUS       DBNGVD010000338         2122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1717259, whole genome shotgun sequence.
ACCESSION   DBNGVD010000338 DBNGVD010000000
VERSION     DBNGVD010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1717259"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16CM_09050"
     CDS             complement(<1..299)
                     /locus_tag="AC16CM_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3140019.1"
                     /translation="MRIVVVGATGNLGTSVLRSLEKEDSVESVLGLARRLPGMTMPKV
                     EWAAADVVDDDLVPYFRGADAVVLLAWVIQPSRDLTRQWMINVEGSTRVARAVKEA"
     gene            410..865
                     /locus_tag="AC16CM_09055"
     CDS             410..865
                     /locus_tag="AC16CM_09055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140020.1"
                     /translation="MELREITADNWRAVVGIEPRGDQRRFVATVAYYLNLCHYGGVWQ
                     PFALYGGDEPVGFAMWGYDADEDSHWIGGLVIDAKHQGRGYGRSAMEAVLDHLARQPG
                     FREAALSYEPENTVARKLYMSLGFVETDEWVDNERVARRPAYNKTTRGG"
     gene            867..1775
                     /locus_tag="AC16CM_09060"
     CDS             867..1775
                     /locus_tag="AC16CM_09060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3140021.1"
                     /translation="MQVLVTGARGKVGTATVAALLEAGHNVRATDLTPPVFERPEEGE
                     ADYYQADLTDAGDAFAVVRGAEAVVHTAAIPEPTRNPPHVVFQNNLMATFNVLEAAIR
                     FGVTRFVNTSSETVPGFFFPERPVLPDYVPVDEDHPIRPQDPYATAKYFGELLMDGAI
                     RRSDIRCITIRPSWVQHEGNYERNLGPMLRDPSELSAGFWSYIDVYDLADAMVLAAES
                     DLPGHEVFYIASPDNVGGRSFEAAVLEHYGEEVEVKTPLAREDASGISIEKARRMLGY
                     DPKRSWRDYLDEEGHLKPGVEGLFSS"
     gene            complement(1865..>2122)
                     /locus_tag="AC16CM_09065"
     CDS             complement(1865..>2122)
                     /locus_tag="AC16CM_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3140022.1"
                     /translation="HGRGSWVEVEVPNEPGTLSKVTDVIRDRKVNIGGVFLGPADRET
                     NRVIVLRLETGNPSGTVRALEDAGYRVTTVESSPNPTLEEA"
BASE COUNT          392 a          685 c          676 g          369 t
ORIGIN      
        1 gcctccttta cggccctcgc gacgcgcgtg cttccttcga cgttgatcat ccactgcctg
       61 gtgaggtcgc gggaaggttg tatcacccag gcgagcagca ccacggcgtc ggccccacga
      121 aagtaaggaa ccaggtcgtc atcgaccaca tcagccgcgg cccactccac ctttggcatc
      181 gtcatccccg gcaaccggcg cgccaggcca agtacggact cgacgctgtc ttccttctcg
      241 agcgaccgga gtacgctcgt tccgaggttg cccgtcgccc ctacgaccac gattctcatg
      301 ctcggccttt accccgtgtc cgggcgggcg aaaccgctgt cgcccccgac gccggtctgc
      361 tgtagtatct gccgtgggct cgaagaagga aggaacataa cctggccgta tggagcttcg
      421 agagatcacc gcagacaact ggcgcgccgt ggtcggcata gagccacgcg gggatcagcg
      481 acgtttcgtg gcgaccgtgg cctactacct caacctctgc cactacggtg gggtgtggca
      541 acccttcgcc ctctacggtg gcgacgagcc cgtcggtttc gcgatgtggg gatacgacgc
      601 ggatgaggac agccactgga ttggggggct cgtcatcgat gcgaaacatc agggtcgagg
      661 ctacggacga agtgcgatgg aggcggtcct ggatcacctc gccaggcagc cagggttcag
      721 ggaggccgcc ctctcctacg agccggagaa cacggtagcc cgaaaacttt acatgagcct
      781 ggggttcgtc gagacagacg agtgggtgga caacgagcgc gtcgcgcgcc gtccggcgta
      841 caacaagaca acacgaggag gataagatgc aggtactggt aacgggagcg aggggcaagg
      901 tcggaaccgc gacggtggcg gcgctgctgg aggctggtca taacgtgcgg gcgacggacc
      961 tgaccccgcc ggtcttcgaa cgccccgagg agggcgaggc ggactattat caggcggatc
     1021 tgaccgatgc gggcgacgcc ttcgccgtgg tgcgcggggc cgaggcggtg gtccacacgg
     1081 ctgccatccc cgagccaacc agaaatcccc cgcacgtggt atttcagaac aacctcatgg
     1141 ccaccttcaa cgttctggag gcggccatcc ggttcggggt cacccgcttc gtgaacacct
     1201 ccagcgagac ggtgccaggc ttcttcttcc ccgaacgacc cgtgcttccc gactacgtgc
     1261 ccgtcgacga agatcacccg atccgcccgc aggaccccta cgcaaccgcg aagtatttcg
     1321 gcgagctgtt gatggatgga gccattcgtc gctcggacat ccggtgcatc acgatccggc
     1381 cctcctgggt gcagcacgag ggcaactacg agcgcaacct gggcccgatg ctgcgcgacc
     1441 cctcggagct cagcgcgggc ttttggagct acatcgacgt ctacgacctg gccgacgcca
     1501 tggtgctggc cgctgagagc gacctgccag gccacgaggt cttctacata gcctcaccgg
     1561 acaacgtcgg aggcagatcg ttcgaggccg ccgttcttga gcactacggg gaagaggtcg
     1621 aggtcaagac ccccctcgca cgcgaagacg cctcgggcat ctctatcgag aaggccagac
     1681 gcatgctcgg ctacgacccc aaacgctcct ggcgcgacta cctcgacgag gagggtcacc
     1741 tcaagccagg ggtcgagggc ctcttctcga gctaggcagg gtgcgaagaa ggcgtcaggc
     1801 tacaggtttc gggcttcagg aaaattacct gtaacctgcg gcttgatgcc tgtagccctt
     1861 cgacttatgc ttcttccagg gttgggttcg gggaggactc gacggtggtc accctgtagc
     1921 cggcgtcttc gagcgcgcgg accgttcccg aagggtttcc ggtctccagg cgcagcacga
     1981 tgacacggtt ggtctcgcgg tcggcggggc cgagaaagac gccgcctatg ttcaccttac
     2041 ggtcccggat cacgtccgtg actttggaca gcgttcctgg ctcgttcggc acctcgacct
     2101 cgacccaaga ccccctgccg tg
//
LOCUS       DBNGVD010000339         3716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3538976, whole genome shotgun sequence.
ACCESSION   DBNGVD010000339 DBNGVD010000000
VERSION     DBNGVD010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3538976"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            785..857
                     /locus_tag="AC16CM_09070"
     tRNA            785..857
                     /locus_tag="AC16CM_09070"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:819..821,aa:Glu,seq:ttc)
     gene            881..952
                     /locus_tag="AC16CM_09075"
     tRNA            881..952
                     /locus_tag="AC16CM_09075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:913..915,aa:Val,seq:tac)
     gene            complement(1298..1942)
                     /locus_tag="AC16CM_09080"
     CDS             complement(1298..1942)
                     /locus_tag="AC16CM_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140023.1"
                     /translation="MTPIRVLVADDPPVFRDGLKTLLESAPDTEFVGEAETGGQAIEM
                     AEDLQPDVILMDVQMPDGGGIEATRRISRDGPQIQVLIVTMFEDDATVFQAMRAGARG
                     YLLKGANSTEMLRAIRAADNGEAIFSPKVAVRLMDFFSSARLSAPPEIFPELSERERE
                     ILDLIAQGYKNPEIAADLYLSPKTVRNHVSNILHKLQVADRAEAIIRAREAGLG"
     gene            complement(1945..>3716)
                     /locus_tag="AC16CM_09085"
     CDS             complement(1945..>3716)
                     /locus_tag="AC16CM_09085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3140024.1"
                     /translation="PNAMSALSMEYAPLWWPISLLNFLGAASFGLFLYLFPDGRFVPG
                     WTRWVVFAWIAWLLPTYWFPGWDSFDLDGWPDWLAIALWTAFLGTMIYAQTYRYWRVS
                     GATERQQIKWVVFGISVAALVYLGIVVALSPFAPAPATPGMVVNALIGYTFLYAAMLL
                     VPLSIGFAILRHHLFDIDLIINRTLVYGALTASVVALYVLVVGALGQFLQVSGNLIVS
                     LIATGLTAVLFQPLRTRLQRGVNHLMYGERDEPYAVLSRLGQRLEASLTPDAMLPTVA
                     RTVAEALKLPYVAIERGHDGAMETTAIGKPVKDPVRLPLTYGGETVGQMVLGPRAGGP
                     FTPADAALLEDLAHQTAIAVHAVTLHREAVRLSADLQRSRELLVTAREEERRRLRRDL
                     HDGLGPRLAAQSLKVGSARMLYDRDPKTADTLLSELESDIEATLAEIRRLVYDLRPPA
                     LDELGLVGAIRDAAERHSSQAADGLHVSVHAPDKLPPLSAAVEVASYRIVQEALANVT
                     HHAGATDCVVRLTVAGELRIEVSDDGVGIPKEHHSGVGLHSMRERAEELGGTCIVGRS
                     ASEGTRVLARLPLPDRKKLASLE"
BASE COUNT          736 a         1114 c         1211 g          655 t
ORIGIN      
        1 ttgctgcgac cacactaggg ttgctcgctt gaataggcgt tgtacgagga ttccgaccaa
       61 ggtgtggtct cggtagctag agccggtgcc cgtagtgggg tgctgggaaa gggaaaggag
      121 gatgcgggca ccggctacgg ctcttcatac agcatatatt cgtgcgagtg aagcctttcg
      181 gacgtatgcg ggacaaattg actagacaga tatagggcaa actgagcatg taagcagatg
      241 aacggcgcga tacgcttcaa tgcaagccga acgggcctac tcacgtactg agtggtcggt
      301 tcgtctctgt gaggtcggtg acagtggagg gggagccgac ctcactggtt tcctgacgaa
      361 ctgcgccact gtgccacggg ctggggccga agacacgata cctacatagc aagtgcccag
      421 cagttaaccg gcctcgcacg ttcccttgta gactcaactt tcgggtgcaa tctcttggaa
      481 cggtgccacg tcgttacccg cgaagtttga caccacgtta tcagcgggcg gaacctgtac
      541 atcaaacctt tcgttagtat cctcattccc ccgatcctct tcatcgtcat ctccctcatc
      601 accctgctct ctgtcgaact ttgaccacgc tgcgctcctt tcgtgctaat ggtccggtgt
      661 cgcagtgtgg ggtcagaagt gggatcaaga cttctggctc acccagtcat cggcgtcgag
      721 tggcgggcat cagccatggc gctatctgtt aaaatcccgg aggcgggacc caccaggctc
      781 gtaagccccc atcgtctaga ggcccaggac accgcccttt caaggcggcg gcacgggttc
      841 gaatcccgtt gggggcacag actgactggc cagccaggac gggcgattag ctcaggggta
      901 gagcgcctcc cttacaagga ggaggtcgct ggttcgaacc cagcatcgcc cacatagaaa
      961 aaacggcgtt ttgcaggtga aacgtcgaaa agaagggaag gccgggcagg aagcctagtc
     1021 ccttctacat cacctctgat ggaagtcggc cgccctccgc gctgcaaggg tccgggctgt
     1081 aaccgcatca tacgcatagg gaagtacgcg cccggagatc gtgaactcgc agaggagtcc
     1141 aggcacaggg gcgaccgtca tcgacgctac aagacccgaa aagacaagga gtattgctcc
     1201 gacaattgca gggtaaaggc tcactaccat gagatcgtga agccacggaa acagaaacgg
     1261 gccgcaaagt catgacaccg ccgctgcatc aatcagacta ccccaatccc gcttcgcggg
     1321 cgcggatgat cgcctcggcg cgatccgcga cctgtagctt gtgcaggatg ttcgagacgt
     1381 ggttgcggac ggtcttgggg ctcaggtaaa gatcggcggc tatctcgggg ttcttgtagc
     1441 cctgggctat aaggtccagg atctctcgct ctcgctcgct cagctcggga aagatctcgg
     1501 gtggcgccga tagcctagcg cttgagaaga aatccatcag gcgcaccgcc accttaggac
     1561 tgaagatggc ctcaccgttg tcggcggccc ggatggcacg cagcatttcg gtggagttgg
     1621 cgcccttgag gaggtagcca cgtgctccgg cccgcatcgc ctgaaagacc gtggcatcgt
     1681 cctcgaacat ggtgacgatc aggacctgta tttgcgggcc gtcacgtgag attctacgcg
     1741 tcgcctcgat gccacccccg tctggcatct gcacgtccat caggatcacg tcgggctgga
     1801 gatcttcggc catctcaatg gcttgcccgc cggtctcggc ctccccgacg aactcggtgt
     1861 cgggggccga ttcgaggagc gttttcaagc cgtcccggaa gacaggaggg tcgtcggcga
     1921 cgagtacacg aatcggtgtc atgctcactc cagcgatgct agctttttgc gatccggcag
     1981 cggcagacgg gccaggaccc tggttccttc cgaagcggag cgcccgacaa tgcaagtccc
     2041 gccgagctct tcggcccgtt cgcgcatgga gtggaggccc acgccggaat ggtgttcctt
     2101 cggtatcccg accccgtcgt ctgaaacctc gatccgcagt tcgcccgcga ccgtgaggcg
     2161 aaccacgcaa tccgtggcgc cagcgtgatg ggtgacgttg gcgagcgctt cttgcacgat
     2221 gcggtaggag gcaacctcga ccgcggcgga caggggcggg agcttatccg gcgcgtggac
     2281 cgagacgtgg agcccgtctg cggcttgcga ggagtggcgt tccgcggcat cacgaatcgc
     2341 acccaccagg cccaactcat cgagagccgg aggacgcagg tcgtagacga ggcgccggat
     2401 ctcggcgagc gttgcttcta tgtccgattc gagttccgaa agaagcgtgt ccgccgtttt
     2461 ggggtcccga tcgtagagca tccgcgccga gcccactttg aggctctgcg cggcaagcct
     2521 tggaccgagg ccgtcgtgca gatcgcggcg gaggcggcgg cgctcctcct cccgtgcggt
     2581 gacaaggagc tcgcgcgagc gctgcaggtc ggcggagagc cgcacggctt cgcggtggag
     2641 ggttaccgcg tggacggcga ttgccgtctg gtgcgccagg tcctcgagca gcgcggcgtc
     2701 cgccggcgtg aagggtccgc ctgcccgcgg tcccaggacc atctgcccga cggtctcccc
     2761 accgtaggtg agcggtaacc ggacggggtc cttcaccggc ttgcctatcg ccgtcgtctc
     2821 catcgcaccg tcgtgccccc gctcgatggc gacgtaaggg agcttcaagg cttcggccac
     2881 cgtacgcgcc acagtgggca gcatcgcgtc gggagtgagg ctcgcctcca ggcgctggcc
     2941 gaggcgcgag agcaccgcgt agggctcgtc gcgctctccg tacatcaggt ggttgacccc
     3001 gcgctgcagg cgtgtgcgga gcggctggaa gagcaccgcc gtcaggccgg tcgcgatgag
     3061 agagacgatc aggttcccgc tgacctgcaa gaactgcccc aacgccccga cgaccagcac
     3121 gtagagggcg acgacgctgg ccgtgagcgc gccgtagacc agcgtgcggt tgataattag
     3181 gtctatgtcg aagaggtggt ggcgcaggat cgcgaagcct atggagagtg ggacgaggag
     3241 catcgctgcg tagaggaacg tgtacccgat cagggcgttg accaccatgc caggtgtcgc
     3301 gggagccgga gcgaaagggg agagcgccac gacgataccg agatatacca gggctgccac
     3361 cgagatcccg aagacgaccc atttgatctg ctggcgctcc gtcgcgcccg agacacgcca
     3421 gtagcggtaa gtctgcgcgt agatcatcgt gcccaggaaa gccgtccaca aggctatcgc
     3481 caaccagtcc ggccagccgt cgagatcgaa ggaatcccag cccgggaacc agtacgtggg
     3541 cagtagccag gcgatccatg cgaagacgac ccagcgagtc cagccaggta cgaaacggcc
     3601 gtcggggaag aggtacagga acaggccgaa ggaggctgcg ccgaggaagt tcaggagaga
     3661 tatgggccac cacaaggggg cgtactccat agagagtgcg gacatagcgt tcggta
//
LOCUS       DBNGVD010000340         3880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3539019, whole genome shotgun sequence.
ACCESSION   DBNGVD010000340 DBNGVD010000000
VERSION     DBNGVD010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3539019"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..867
                     /locus_tag="AC16CM_09090"
     CDS             118..867
                     /locus_tag="AC16CM_09090"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HIR3140025.1"
                     /translation="MRALVIVGHGSHLNGDSGLPVYQHAERIRETGEFDEVVECFWKE
                     EPSMRHVLDTVESEDVYVVPAFISEGYFTQQVIPRELGLAGPVTSKGGKTIRYAGPLG
                     TFEGMPDVILERTDDLMRDKGVSGRTALVLLGHGTDLNKNSAGVIYLNAERIRERGVY
                     DLVEVGFLDQEPEIGGVVDGVNADNVILIPLFIAEGWHTRETIPEDLGLTGEVTVRDD
                     RTIYYGAPVGTHPSMADLISARARMTEGQVA"
     gene            860..1681
                     /locus_tag="AC16CM_09095"
     CDS             860..1681
                     /locus_tag="AC16CM_09095"
                     /inference="COORDINATES: protein motif:HMM:NF037718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HIR3140026.1"
                     /translation="MPEGVSGRPGPENTATASREAFARWVDEAPEGRVFGQVILSPTS
                     AGGYALRHAEDASVRELQTSDDPRHAREIARLTAEGAYRPLKSSPNLRRGWELRVADA
                     RSLGTAMDYLYPAGVVHWHLLREGMLTLTTFRQNAARQSGIYQRTRRLTDRGVQDAAR
                     ACCEDQVCLKKTLWDVDENTPLDMERGEGEIPCPEPCSIFVSFARRVRFFEREKERDA
                     DGLSSSEREDLAALVEAAASGEVRFAREAEFEEPLNERRMRYRRLTLVPTLRAED"
     gene            1728..1922
                     /locus_tag="AC16CM_09100"
     CDS             1728..1922
                     /locus_tag="AC16CM_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3140027.1"
                     /translation="MLVQLVRGALGKSGKRTSGINVSSCSFVVTLHGSVGSVEEGHEI
                     EEIARSVPGVEAVSNKLLVR"
     gene            2006..2680
                     /locus_tag="AC16CM_09105"
     CDS             2006..2680
                     /locus_tag="AC16CM_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3140028.1"
                     /translation="MASKKARGARAGAKAGARIAPKAGRTAGKLAWKAGKSEAKLIRR
                     AMSAQQSRKSRYFKYAIFTLIGLAIGAAISRSKQEGGTSGASSSYTGTTGPHAPDSGS
                     PAGQRGETWGSGTPTGTAGGGGAPGTGAARDYSDPSSGPLIGEHHEPVEGVGEEQDEL
                     EQRIRTNIGEDPRTAEMPRVNVEVNDGVAELRGVAPSEDAKLAAGEIAQAVEGVREVR
                     NLITVS"
     gene            complement(2816..>3880)
                     /locus_tag="AC16CM_09110"
     CDS             complement(2816..>3880)
                     /locus_tag="AC16CM_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3140029.1"
                     /translation="TASHNPKDNNGFKLCREDAIALSGDAGLGEIRKLIVDGDLPGVA
                     EYSGSVEETDITGDYAEHCLSFIDTEGLRPLKIVIDAGNGMAGKMVPPIFERLPFEYV
                     PMYFELDGSFPNHPPNPIEPENVEELQERVVAEGADFGVAFDGDADRCFIIDEKGTTI
                     SGDLAATLIAKNVLEKEPGATILYSAVCSKAFPELVEREGGQAIRTKAGHSIIKPQMR
                     KYDAAFGGEHSAHFYFRDNYFADSGIIAMLTVAELVGRQEAPISELLAPIDPYVRSGE
                     INSEVEDQVAVIKKAQDRFSSAEDVKIDYLDGLTVDLGDWWFNLRPSNTEPLLRLNVE
                     ASDRETMEQRRDELLELIRE"
BASE COUNT          728 a         1187 c         1289 g          676 t
ORIGIN      
        1 cccttcacga gccaccagct ctcagccctc ggcaagtgaa tctgatagct gaaagctgac
       61 ggcttagagc tttcaattga tagaatcaga tagcaccgta gacactagag gagtcacatg
      121 agagctctcg tcatcgtggg acatggttcg cacctcaacg gggattcggg cctgcccgtc
      181 taccagcacg cggagaggat cagggagacc ggggaattcg acgaggtcgt ggagtgcttc
      241 tggaaggaag agccatccat gcgccacgtg ctcgatacgg ttgagtccga ggatgtctac
      301 gtggtccccg cattcatctc cgagggatac ttcacccagc aggtgatccc gagggagctg
      361 gggctagcgg gaccggtgac gagcaagggc ggcaagacca tccggtacgc tggcccactc
      421 ggcaccttcg aagggatgcc cgacgtgata ctggaacgca ccgatgacct catgcgcgac
      481 aagggggtct ccggtcgcac ggccctcgtc ttgctcggcc acggcaccga cctgaacaag
      541 aactccgcgg gcgttatcta cctgaacgca gagcgcatca gggagcgggg ggtctacgac
      601 ctcgtagaag tagggtttct ggaccaggaa cccgagatag gtggcgtggt ggatggcgtg
      661 aatgcggata acgtgatcct gatccccctg ttcatcgcgg agggctggca cacccgtgag
      721 accattccgg aagacctcgg tcttaccggt gaggtcacgg tccgggacga caggacgatc
      781 tactatggcg ctcccgtggg cacgcacccg tcgatggccg acctcatctc ggcccgcgca
      841 aggatgacgg aggggcaggt tgcctgaggg cgtctccggg cggcccggcc ccgagaacac
      901 cgccacagcc tcgcgtgagg ctttcgcccg atgggtagac gaggcaccag aaggccgcgt
      961 gttcgggcag gtgatcttga gtcctacttc ggcgggcggc tacgcgcttc gccacgcgga
     1021 ggacgcaagc gtccgagaac tccagacgag cgacgatccc cgccacgccc gagagatcgc
     1081 ccgtctcacg gccgaaggcg cgtacaggcc cctcaagagc tcgccgaacc tgcggcgggg
     1141 ctgggagcta cgagtcgccg acgcccgctc cctcggaacc gcgatggact acctgtatcc
     1201 tgccggcgtc gtccactggc atctcctcag ggagggtatg ctcaccctga cgaccttcag
     1261 gcagaacgcg gcgcggcaga gcggcatcta ccagcgcacg cggcggctca ccgaccgggg
     1321 cgtgcaggac gcggcccgcg cctgctgcga ggatcaggtt tgcctcaaga agacgctctg
     1381 ggacgtggac gagaacacac ccctcgacat ggagaggggt gagggtgaga tcccctgccc
     1441 cgaaccctgc agcatcttcg tctctttcgc gcgccgcgtg aggttcttcg agcgggagaa
     1501 ggaacgcgat gcggacggtc tttcgtcctc ggagcgggag gacctcgccg ccctcgtcga
     1561 ggccgcggca tcgggggaag tcaggtttgc ccgcgaggcc gagttcgagg agccactcaa
     1621 cgaacgcaga atgcggtaca ggcggctgac gctcgttccg acgctacgcg ccgaggatta
     1681 gcacggccac ggagtaccgg tccgcagatc ccccggcaaa tgatgcgctg ctcgtgcaac
     1741 tggtacgtgg ggcgctggga aaaagcggca agcgcacctc ggggataaac gtgagctctt
     1801 gctcgttcgt ggtcaccctg cacgggagcg tgggttccgt cgaggagggg cacgagatag
     1861 aggagattgc tcgttcggtt cccggtgtcg aagccgtgtc gaacaagctt ctggtgaggt
     1921 aggtccccct ggctgtttcc gaaggcacca ttcagggtat ctcgatgttt ccagagaaca
     1981 tactccgaaa ggacggaggt aagcgatggc cagcaagaaa gcaagaggtg ccagggccgg
     2041 tgcgaaagcg ggggcaagga tagccccgaa ggcgggtcgg accgccggca agctcgcgtg
     2101 gaaggccggc aagagcgagg cgaagctgat ccgcagggct atgagcgccc agcaatccag
     2161 aaagtctcgc tacttcaagt atgcgatctt caccctgatc gggctcgcca tcggggcggc
     2221 aatcagccgg tcgaagcagg aaggcggaac ttcgggcgct tcctcgtctt acacgggcac
     2281 gactggacca cacgcacccg attcgggcag cccggccggc cagcgggggg agacctgggg
     2341 tagcggaacc cccacgggta ccgcgggcgg tggcggagcg cctggcaccg gtgctgcgcg
     2401 tgactactcc gacccctcca gcggacccct gatcggcgaa catcatgaac ccgtcgaggg
     2461 tgtaggcgag gagcaggacg agctcgaaca gcgcatccgc accaacatcg gcgaagatcc
     2521 gcgtacggcg gagatgcccc gcgtaaacgt cgaggtgaac gacggcgtcg ccgagctgcg
     2581 cggtgtggcc ccctccgagg acgccaagct ggcggccggc gagatcgccc aggccgtgga
     2641 gggcgtgcgc gaggtgcgta acctcatcac cgttagctag aaaataggca tcagggttca
     2701 ggctacaggc atcagcctgc aaagcctcgc ccgatgagaa gaagcccccg ccattccggg
     2761 cgggggcttc ttcgcgttgt ggggcgctac gccccctatt cctgaaacct gtagcctact
     2821 cccgaatcag ttcgagcaac tcgtctcgcc gctgctccat ggtctctcta tcgctggctt
     2881 ccacgttgag gcgcaggagc ggttccgtgt tcgagggacg gaggttgaac caccagtcgc
     2941 ccagatctac ggtcaggccg tcgaggtagt ctatctttac gtcctccgcg ctggagaagc
     3001 gatcctgtgc cttctttatc accgctacct ggtcttcgac ctcggagttg atctcgcccg
     3061 agcggacgta ggggtcgatg ggagctagga gctcggagat cggggcttcc tgcctgccta
     3121 ccagctcggc gaccgtgagc atggcgatta tgcccgagtc ggcgaagtag ttgtccctga
     3181 agtagaagtg ggccgagtgc tccccaccga atgccgcgtc gtacttgcgc atctgaggct
     3241 tgatgatgga gtggcccgcc ttggtcctta tagcctgtcc gccctcccgc tccacgagct
     3301 ccggaaaggc tttcgagcag acggcactgt agaggatggt agccccgggc tccttttcaa
     3361 gcacgttctt ggcgataagg gtggccgcga gatctcccga gatggtcgtg cccttctcgt
     3421 cgatgatgaa gcagcggtcc gcatctccgt cgaaggcgac cccgaagtcg gcgccctctg
     3481 cgaccacccg ttcttgcagc tcttccacgt tctccggttc tatggggttc gggggatggt
     3541 ttggaaaaga gccgtcgagc tcgaagtaca tcggcacgta ctcgaagggg agtcgctcga
     3601 agatgggggg aaccatcttg cccgccatgc cgttgcccgc gtcgatgact attttgagcg
     3661 gcctcaggcc ctccgtgtcg atgaagctga ggcagtgctc ggcgtagtcg cccgttatat
     3721 cggtctcctc gacggaaccg gagtactcag ccacacccgg caggtctcca tcgacgatca
     3781 gcttccggat ctcgcccagg cccgcgtcgc cggagagggc tattgcgtcc tcgcggcaga
     3841 gcttgaagcc gttgttgtcc ttggggttgt gggaggcggt
//
LOCUS       DBNGVD010000341         3586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3903585, whole genome shotgun sequence.
ACCESSION   DBNGVD010000341 DBNGVD010000000
VERSION     DBNGVD010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3903585"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="AC16CM_09115"
     CDS             complement(<1..1874)
                     /locus_tag="AC16CM_09115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3140030.1"
                     /translation="MARTHRESGRRGRGRAGRPVRLPKHRKRRTGTPNGPAARFLRFL
                     GLLFLCAVVAAVAFAAGAYLGLIAGVERLGAPRAQETYPTYIYSAPLGDNENSRRVIG
                     TILEGQNRKTASLSEMPPQLLNAVVAKEDERFRTHGGVDLWGIMRALYVDIRAGEAVE
                     GASTITQQYVRNAYLTQDRSITRKVKEALIAIEVERKEDSKDQIIADYLNTVYFGSAA
                     YGVEAAAETYFNKSVEDLSIAESATLVGLLWSPSTLGEDREGALSQRNLVLRKMFDTG
                     YITSEDYNAALEAPMPENWPMDPPAQSGFSGPGLTRNFADYAHDELIRRYGANTVLQG
                     GLSVYTTIDLEDQVAARDIAYGSSGYLPNADDPDLALVSIEPRTGHIKAMLGDRDENA
                     QFSLATQGRRQPGSSFKVFALIAALEQGIDPETTYVSEEKEYKIDVGLDKPEPWRVQN
                     YDGIVRGRISLEEALWYSDNTVFADLVMNADGRGLKDGPEKIAEVAEKCGITVDLPRH
                     PKPSIVLGAYEVSPLDMASAYATIANGGRRVEPTAITRVVSDEGREDEKVLYQEPDQP
                     EGEQVIPVDIAHKATEIMVGDVTQGIAGDAALEDRPVAGKTGTSENFFDAWFVGYTPQ
                     L"
     gene            1942..3117
                     /gene="tyrS"
                     /locus_tag="AC16CM_09120"
     CDS             1942..3117
                     /gene="tyrS"
                     /locus_tag="AC16CM_09120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3140031.1"
                     /translation="MNEDSANLSIYKHVVDVIPREELDRRLASGETLRVKLGIDPTAP
                     DIHLGFAVVLRELRAFQDLGHVAVLVIGDYTARVGDPSGRSKERPILSPEQIEANTRT
                     YLEQAYLILDRERTEVRRNSEWLAPLTMADIIGLTRATTVARVLERDDFARRYAAKSP
                     ITLTELLYPLMQAYDSVAIDADVELGGTDQLYNLLMGRQVMEYYGMEPQCVVTTPLLV
                     GTDGKAKMSKSVGNYIGVTDPPAQMFGKAMSIPDGLMPDYYSLLLDRPMPTAEPVQQK
                     RGLARALVQTFHGDAAVADAEKTFDAVVRREMPEDAPVVELPGSTEIWIVDLITLAGF
                     ATTNGEARRFIRGGAVRVGGEVVRDEKVSFGTGELDGAVLQVGKRRYAQLVSKKVED"
     gene            3461..>3586
                     /locus_tag="AC16CM_09125"
     rRNA            3461..>3586
                     /locus_tag="AC16CM_09125"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          642 a         1079 c         1163 g          702 t
ORIGIN      
        1 agctggggag tatagccaac gaaccaggca tcgaagaagt tctcgctcgt tccggtcttg
       61 ccggccacgg gacggtcttc gagcgcggcg tccccggcga tgccttgcgt gacgtctccc
      121 accatgatct ccgtcgcctt gtgggcgata tccactggaa ttacctgctc cccttcgggc
      181 tgatctggct cctggtacag gaccttctcg tcctcgcggc cctcgtcgct cacgacccgc
      241 gtgatcgccg tcggctcgac ccttcttccg ccgttggcga tggtggcgta ggccgaggcc
      301 atatccagtg gcgatacctc gtaggccccg agcacgatcg atggtttggg gtgcctggga
      361 aggtccacgg tgatgccgca cttctctgcg acctcggcga tcttctccgg gccatctttc
      421 agaccacggc cgtcggcgtt catcaccagg tcggcgaata ctgtgttgtc cgagtaccag
      481 agcgcctctt caaggctgat cctgccgcga acgatgccat cgtagttctg gacgcgccag
      541 ggttcgggtt tgtctagccc cacatcgatc ttgtactcct tctcctccga cacgtacgtg
      601 gtctccggat ctataccctg ctcgagggcc gcgatgaggg cgaagacctt gaaggaactg
      661 cccggctggc gcctgccctg cgtggcgagg ctgaactggg cgttctcgtc cctgtcgccg
      721 agcatggcct tgatgtggcc cgtcctcggc tcgatagaga cgagggcgag atctgggtcg
      781 tcggcgttag gcaggtaacc gctcgacccg tacgcgatgt cgcgggccgc gacctggtct
      841 tcgaggtcga tggtcgtata gacgctgagg ccgccctgca gcacggtgtt ggccccgtag
      901 cgccttatca gctcgtcgtg cgcgtaatcg gcgaagttgc gggtgagccc ggggcccgag
      961 aaccccgact gggccggcgg gtccatgggc cagttctccg gcatcggggc ctccagggcc
     1021 gcgttgtagt cctcgctcgt gatgtatccc gtgtcgaaca tcttcctcag caccaggttt
     1081 cgctgggaca gtgccccctc acggtcctcc cccagggtgg agggggacca gaggagcccg
     1141 acgagggtcg cagactccgc tatgctcagg tcttccaccg acttgttgaa gtaggtctcc
     1201 gccgcggcct ccaccccgta ggcggcgcta ccaaaataga ccgtgttcag gtaatcggcg
     1261 atgatctgat ccttcgagtc ctctttgcgc tccacctcga tggcgatgag cgcctccttg
     1321 accttcctgg tgattgacct gtcctgagtc aggtaggcgt tcctgacgta ttgctgggtg
     1381 atggtgctcg ccccctccac cgcctccccg gccctgatgt ccacgtagag ggcgcgcatt
     1441 attccccaca ggtcgacgcc accatgcgtc ctgaagcgct cgtcttcttt ggcgactacg
     1501 gcgttgagca actgcggcgg catctccgag agcgaggcag tcttgcggtt ctggccctcg
     1561 aggatagtgc cgatgacccg cctcgaattc tcgttgtcgc cgagcggtgc cgagtatata
     1621 taggtcggat aggtctcctg ggcccgggga gccccgagcc gctctacccc ggcgatgagc
     1681 cccagatagg cgcccgccgc aaaggcgaca gcagcgacga cagcgcataa gaacaacagg
     1741 cccaggaagc gtaggaacct ggcggccggg ccgttcggcg tacccgtccg cctcttgcgg
     1801 tgcttcggga gacgaacggg gcgacccgcc cttcctctcc ccctgcggcc ggactctctg
     1861 tgggtgcggg ccatcgagcc gctatagtac actacgtcgg acgaggttac attcatatta
     1921 cgagaaggtt tagatctgtt aatgaacgag gattctgcca acctttccat ctacaagcac
     1981 gtggttgacg tgatcccccg ggaggagctc gaccgtcgcc tggcgagtgg cgagaccttg
     2041 cgggtcaagc taggaatcga cccgacggcg ccggacatac atctgggctt cgcggtggtg
     2101 ctcagagaat tgcgggcgtt tcaggatctg gggcatgtgg ccgtgctcgt cataggcgac
     2161 tatacggcgc gggttgggga cccttcggga cgttccaagg agcgtcccat cctctctccg
     2221 gagcagatcg aggcgaacac ccgaacgtac ctcgagcagg cgtatttgat actggatcgc
     2281 gagcgaaccg aggtgaggag gaattcggag tggctggcgc cgctcacgat ggccgacatc
     2341 atcgggctta cgagggcgac gacggtggcc cgcgtgctgg agcgcgacga tttcgccagg
     2401 cgttacgcgg cgaagagccc tataacgttg accgagctgc tgtatcccct gatgcaggcc
     2461 tacgactccg tggcaatcga cgccgatgtc gagcttggtg gcactgacca gctctacaac
     2521 ctgctcatgg gtcggcaggt gatggagtac tacggtatgg agccacagtg cgtggtgacg
     2581 accccgctcc tcgtcggtac ggacgggaag gcaaagatga gcaagtccgt gggcaactac
     2641 ataggcgtca ccgacccacc ggcccagatg ttcggcaagg ccatgagcat ccccgacggg
     2701 ctcatgcccg actactactc gctgttactg gacaggccca tgcccacggc cgagcctgtg
     2761 cagcagaagc gcgggttggc cagggcgctg gtccagacgt ttcacgggga cgctgcggta
     2821 gccgatgccg agaagacctt cgacgccgtc gtgcgccgcg agatgcccga ggatgcgccc
     2881 gtagtggagt tgccgggcag cacggagatc tggatagtcg acctcataac tctggcgggg
     2941 ttcgccacca caaacggcga ggctcgccgc tttataagag gcggcgcggt ccgtgttggc
     3001 ggggaagtcg tacgggacga gaaggtctct ttcgggaccg gagagctcga tggggcggta
     3061 ctgcaggtcg gcaagcggcg ctacgcccag ctcgtttcaa aaaaggttga agattaggtt
     3121 tgacacccca agaggcggtg ctatacttct ctagctgatg acgggaaacg acttgaaaag
     3181 cgagagcggc atatagggta gggcttccga caaactggga gtacgtacct ggcccgccgc
     3241 aaggtgggcg ttttagtcgc catcgaagaa gagtcgccgt cggctccgcc gagaggcagg
     3301 ggcttgaggc cttgaaaact ggatagcagt aaaagattgg gtagagaacc cgtctaaccg
     3361 ccgagactga ctctctgcgg tccgagagtg taatgcggat cgccgagcgg gtggcgcccg
     3421 cacaacctta actttagccc cgccaagcgt ggggcgagtt agttcggaga gtttgatctt
     3481 ggctcaggac gaacgctgac ggtgtgcttt aggcatgcaa gtcgaacgag aaagcccttc
     3541 ggggtgagta aagtggcgaa cgggtgagta acacgtgggc aacctg
//
LOCUS       DBNGVD010000342         5857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3721779, whole genome shotgun sequence.
ACCESSION   DBNGVD010000342 DBNGVD010000000
VERSION     DBNGVD010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3721779"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..676)
                     /locus_tag="AC16CM_09130"
     CDS             complement(269..676)
                     /locus_tag="AC16CM_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140032.1"
                     /translation="MNLTHEYMHHRTGYSLGAGCCWIRIYKGAEGDAPVVVCEQLPGV
                     GDVATREVSGFLAAEVIREHFSDGLPDLPRPLLWIEHRPARRRGPGKYFLHTFPCYEP
                     RTVGAGFVRRVTLGTSHREALSPSEAAVLTKDV"
     gene            complement(698..1687)
                     /locus_tag="AC16CM_09135"
     CDS             complement(698..1687)
                     /locus_tag="AC16CM_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3140033.1"
                     /translation="MDLLILGGTRFLGRYLVEAALREGHEVTLFNRGISGPDLFPEVE
                     NIKGDRDGDLSSLRGRRWDAAIDTCGYLPRVVRASAGLLADAVDHYTFVSSISVYAED
                     IGPGADEDAPVVELEDPTVEHVTGETYGGLKALCERAAEEEMPGRVLNVRPGLISGPH
                     DPTDRFTYWPRRVAGGGEVLSPDRPEHQVQYIDVRDLASWMVKMAAGCQTGTYNATGP
                     DYILQMGKLLEECEAIGGGGAEFVWVSEEFLEENGVEPFTEMPLWVPREYAGMMEVDC
                     GRAIGAGLTFRPVSETVRDVLEWDARRTGSDPAAGLETGRERELLSAWRGGNL"
     gene            complement(1738..2364)
                     /locus_tag="AC16CM_09140"
     CDS             complement(1738..2364)
                     /locus_tag="AC16CM_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR3140034.1"
                     /translation="MSSDPRLVVPYVTEQSPRGERTMDIYSRLLKDRIVFLGTPVDDQ
                     VANVIMAQLLHLDAEDPEQDIHLYINSPGGSGSAMLAMYDTMQFVSADVATTALGMAA
                     SAGAFLLAAGAKGKRSALPNTRILIHQPNIQGLGGQASDVEIHAREIMNQKRRVNEIL
                     AERTGQDYEKIDRDTDRDYILSSQEAVEYGVIDRVVDRPEQRIPTAGN"
     gene            2466..2744
                     /locus_tag="AC16CM_09145"
     CDS             2466..2744
                     /locus_tag="AC16CM_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140035.1"
                     /translation="MDLPRLRRIRQGAVMSQEELAERSGVARDTISKLETGRRGAYPS
                     TIRKLAAGLEVQPQLLMGGVEYLDESVEGEPVENEPHEKPGRDRKVGF"
     gene            3251..3940
                     /gene="trmB"
                     /locus_tag="AC16CM_09150"
     CDS             3251..3940
                     /gene="trmB"
                     /locus_tag="AC16CM_09150"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HIR3140036.1"
                     /translation="MPRRRLDRKRVPVPDEQASARYLLELPGDRLYREPQAFPKIDPV
                     SLFGNGKPMKLEVGCGDGRFLCSLAKENRETNFVGVEVKQKSVYEAVARAASYGLKNI
                     LFLRANFQLLYPLLIPGSLRAVYLHFPDPNTRPKFRGRRIFSRRFLDEIHAATAPDGR
                     ISVMTDHQGTFMEMLELAESDKPWEKAHAERYLVGFGAERRSRYQRIWEGHGLPTLRF
                     ELVKGGTEMRW"
     gene            complement(3965..5086)
                     /locus_tag="AC16CM_09155"
     CDS             complement(3965..5086)
                     /locus_tag="AC16CM_09155"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HIR3140037.1"
                     /translation="MATVFAAPGKYIQGRGATGRLGEVLEQLGSEKPLVLADPVVEEI
                     MGGALEGLAGATRVEFGGECSPEEIDRVAEAASDAGADAIVGIGGGKTMDTAKSVAHP
                     AGLPLVIVPTIASTDAPTSAVAVVYTEEGEFHEYRFFGRNPDAVIVDTSVIVGAPVRF
                     LVAGIGDGLSTYFEADASSRTRKETMAGGAPTQAALAIARLCYDTLLEHGLAARLAVE
                     QGAVTPAVEKVVEANTLLSGLGFESGGLGAAHSVHNGLTALEGTHDYWHGEKVAFGLI
                     TMLVLEERPAEVLEEVVDFCLKVGLPVSLEDIGVENASREDLEGVAEAACAEGETIHN
                     MPIEIQPAMLVDAMIAADAFGRERRAALEGEASLPAVAN"
     gene            complement(5142..5549)
                     /locus_tag="AC16CM_09160"
     CDS             complement(5142..5549)
                     /locus_tag="AC16CM_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140038.1"
                     /translation="MGIRQVSAVLSQLIVHIPNNIAALTQLGIAVQLTAPTFLIVVRS
                     VALAASIAFGWGARNGAQDIVERAYDRRDEARPEESGGGSAKNPGDGAPGAMAQDTVK
                     ADRAPGVRDPVQSVGVDEKRRDLVCVWTVRSVM"
BASE COUNT          987 a         1971 c         1795 g         1104 t
ORIGIN      
        1 cgcgggcgcg ctggatgtat ttctcggcgg cctcggggtt gttgtagcta agggtgccgc
       61 cggtcatggg gttcttgatc tcgtagtgcc cgccggcctc tctgtccatg atgaccggat
      121 agtatcggtc cccgacctgc tttattgcgt actcggtggt gttgctatct tcgcccaatc
      181 gaactccctc taacgctcac gctaaatata cgtctttcgg gatggcgcgt tttcggtcgg
      241 ttcgcgaacc gcccctgcag gttgcgttct agacgtcttt tgtgagtacg gccgcctctg
      301 atgggctcag ggcttcgcgg tgggaggtgc cgagggttac gcgtcgtacg aagccagccc
      361 ccacggtcct gggttcgtag cacgggaagg tgtggaggaa gtacttgccc gggccgcgcc
      421 tgcgggccgg gcggtgctct atccacagca gtgggcgagg aagatctggt agtccgtccg
      481 agaaatgttc ccttattact tcggccgcca gaaagcccga gacttccctg gttgccacgt
      541 ctcctacccc gggaagctgt tcgcacacca ccacgggggc gtcgccctcg gcgcccttgt
      601 agatcctgat ccagcagcac ccggctccca ggctgtagcc cgtcctgtgg tgcatgtact
      661 cgtgcgtgag gttcattgct ccatccctga aggctcccta caggttgcca ccgcgccacg
      721 ccgagagcaa ctcccgctcc ctccccgtct caaggccggc ggccgggtcc gaccccgttc
      781 tccgggcgtc ccactcgagc acgtctctta ccgtctccga cacgggccga aaggtgagcc
      841 ccgctcctat ggccctgccg cagtcgacct ccatcatgcc cgcatactcc cgcggtaccc
      901 acaacggcat ctccgtgaaa ggctctacac cgttctcttc gaggaattcc tctgaaaccc
      961 agacgaactc cgcgccgcca ccgccgatgg cctcgcactc ctcgagcagc tttcccatct
     1021 gcagtatgta gtcggggccc gtggcgttgt acgttccggt ctgacatcct gcggccatct
     1081 ttaccatcca tgacgccagg tcccttacat cgatgtactg cacctggtgc tctggacgat
     1141 cgggggacag gacctcacca ccgcctgcga ccctgcgcgg ccagtaggtg aagcggtccg
     1201 tgggatcgtg ggggccggag atcagaccgg gacgcacgtt gagaaccctg ccaggcatct
     1261 cctcctcggc cgcccgctcg cagagcgcct tgagcccccc gtaagtctcc cccgtaacgt
     1321 gctcgaccgt tgggtcctcg agctccacca ccggcgcgtc ttcgtcggcc ccgggcccga
     1381 tgtcttcggc atacacggag atgctcgaga cgaaggtgta gtggtccacc gcatcggcca
     1441 acagcccggc ggacgcccgc actacgcgcg gcaggtaacc gcaggtgtct atcgccgcgt
     1501 cccagcgccg tccccgcagg gacgagagat ccccatccct gtcacccttt atgttctcca
     1561 cctcgggaaa caggtcggga ccgctgatcc cccggttgaa tagcgtaacc tcgtggccct
     1621 cccggagcgc cgcctcgacg aggtaccgcc ctagaaaccg cgtgccgccc aggatcaaca
     1681 ggtccatcaa tgcctccagg tggccggggc ggggatctct ccccgccccg gtctcagcta
     1741 gtttccagcc gtcgggatcc gctgttccgg acggtcgacc accctgtcga taacaccgta
     1801 ttcgaccgcc tcctgagagc tgagtatgta gtcgcggtcg gtgtcgcgat ctatcttctc
     1861 gtagtcctgc ccggtgcgct ctgccaggat ctcgttcacg cgccgcttct ggttcatgat
     1921 ctcccgcgcg tggatctcca cgtcggaggc ctgtccgccg agtccctgaa tatttggctg
     1981 gtgtatcagg atgcgcgtgt tgggcaacgc gctcctcttg ccctttgccc ccgccgcgag
     2041 caggaacgcc ccggccgatg cggccatgcc cagcgccgtc gttgccacgt cggcgctgac
     2101 gaactgcatg gtgtcgtaca tcgccagcat ggccgagcca ctaccaccgg gcgagtttat
     2161 gtacagatga atgtcctgtt cagggtcctc cgcgtccaga tgcagtagct gagccatgat
     2221 gacgtttgcg acctgatcat cgaccggcgt gccgagaaag acgatcctgt ccttcaacag
     2281 ccgcgagtag atgtccatcg tccgctcccc ccgcggactc tgctccgtca cataaggaac
     2341 gaccaacctg ggatccgaac tcatcccgta actccctccg tcttggccaa cacgctatac
     2401 tctccataac gtaacattgt tgtaggactt gtgcaagttt tttgcagtgt tttagggagt
     2461 gagtgatgga tcttccgcgt ttgcgtcgca tccggcaggg ggctgtgatg agccaggagg
     2521 agttggctga gaggagtggt gtggcgcgcg acaccatctc caaactcgag acgggtagga
     2581 ggggggctta cccttcaacc atacgcaagc tcgcagccgg cctcgaagtg caaccgcagc
     2641 tactaatggg cggcgtcgag tacctggacg aatcggtcga aggcgagccc gtggagaacg
     2701 agccccatga gaagcctggg cgtgacagga aggtcgggtt ctagggcggc tcttcgtgca
     2761 gaacggagga ggcatcaggc tacaggtttc gggcttcagc aaaatcgcct gtagcctgat
     2821 gccttttttc gagaaccgtc ccacaagcgc gatgcgttgg ggggttcagc acataggcta
     2881 tatgcgggtc ccgtccgggc gctgccttga ccggtatgcg ggcgtctggt agcttgcgct
     2941 ggtgatgaac gagtcgcgac agatctcaaa gggcccgaag agcgggaagg ttttctcccg
     3001 gtaggaggag ttcgtccgct ccaagtaggg ttttcgttat ttacccggca ggccgtgggc
     3061 gctcctcttg cgcccgcggc cttcttttta gcgaacctgg agacccaccg tggggtatcc
     3121 ccggctttcc cgccgtggga acctccgggc ctgggggttt ctggaccctc gcgaaccggg
     3181 ccgcgcggac catcagcccg cggcctgccc ctttccaggt cccccatgtg ggggagcaag
     3241 gggaaaacgc atgcccagaa ggagactgga ccggaaaagg gtgcccgtgc ccgacgaaca
     3301 ggcgtccgcc agatatctac tcgagttgcc aggagaccgc ctctaccgcg agccacaggc
     3361 ctttccgaag atcgaccctg tctcgttgtt cggcaacggg aagcccatga agctggaggt
     3421 cggctgcggg gacgggcggt ttctgtgctc gttggctaag gagaaccgcg agaccaactt
     3481 cgtcggcgtg gaggtgaagc agaagtccgt gtacgaggcg gtcgcaaggg cagcctcata
     3541 cggcctgaag aacatcctct tcctcagggc caacttccag ctcctctacc cgctcctgat
     3601 acctggctcg ctacgcgccg tctacctcca ttttccagac ccgaacacgc gcccgaagtt
     3661 ccgcgggcgg cgtatcttca gccggcgctt cctggacgag atacacgcgg ccacggcccc
     3721 ggacggaagg atcagcgtga tgaccgacca ccagggcacc ttcatggaga tgctggagct
     3781 ggcagagagc gacaagccct gggagaaagc tcacgccgag cgctacctgg tgggcttcgg
     3841 ggccgagagg cgttcgaggt accagcgcat ctgggagggc cacggcctac ccacgctgcg
     3901 cttcgagctc gtcaaaggcg ggaccgagat gcgctggtag ccaccagtca aaaagctgat
     3961 cgctctaatt ggccacggca ggcaggctcg cttctccctc cagggcggcc cgacgctcgc
     4021 gtccgaaggc gtcggcggcg atcatggcgt cgacgagcat tgcgggctgg atctcgatgg
     4081 gcatgttgtg gatggtctcg ccctccgcgc acgccgcctc ggcgacgccc tcgaggtcct
     4141 cgcggctcgc gttctccacg ccaatgtctt cgagggagac agggaggccg accttcaggc
     4201 agaagtcgac gacctcctcc agcacttcgg ccggacgctc ctcgagaacg agcatggtga
     4261 tcaggccgaa ggccaccttc tcgccgtgcc agtagtcgtg ggtgccctcg agggcggtga
     4321 gcccgttgtg gacggagtgc gcagccccca gcccccccga ctcgaagccg aggcccgaga
     4381 gcagggtgtt cgcctccacc accttctcga ccgccggcgt tacggccccc tgctccacgg
     4441 cgagtctggc cgcgaggccg tgctccagca gggtgtcgta gcacagccgg gcgatagcga
     4501 gggccgcctg cgttggcgcg ccccctgcca tggtctcctt gcgcgttcgt gatgaggcat
     4561 ccgcctcgaa gtacgtgctc aacccgtctc ctatcccggc caccaggaat cgcacggggg
     4621 caccgactat aacgctcgtg tcgacgatca cggcgtcagg gttgcgcccg aagaagcggt
     4681 actcgtggaa ctcgccctcc tcggtgtaga cgaccgccac cgcgctggtg ggggcgtcgg
     4741 tcgaggcgat ggtcggcacg ataacgagcg ggagccccgc cggatgggcc acggactttg
     4801 ccgtgtccat ggtcttgccg cccccgatac ccacgatggc gtcggcaccg gcgtccgagg
     4861 cggcctccgc cacccggtct atctcctctg gggaacactc tcccccgaac tcgacccgcg
     4921 tcgcccccgc cagcccttcg agggctccgc ccatgatctc ctcgaccacc ggatcggcga
     4981 gcaccagggg cttctccgag cccaactgct ccaggacctc gcccagcctg ccggtagcac
     5041 ccctaccctg gatgtacttg cccggcgccg cgaacacggt cgccatcgga ccatcgttgg
     5101 actgcatcag ctcgcctcct cagcatagct tgcactctct cttacattac agagcgcacg
     5161 gtccagacgc aaacgaggtc gcgtcgtttt tcgtccaccc cgacgctctg cacgggatca
     5221 cgtactccgg gggctcgatc ggctttcacc gtgtcttgcg ccatggcccc aggagcacca
     5281 tctccggggt tcttcgcgct gccgcctccg gactcttcgg gccgcgcctc gtcgcggcgg
     5341 tcgtaggccc gctcgactat gtcctgggcg ccgttgcggg cgccccagcc gaacgctatc
     5401 gacgccgcaa gggccaccga cctcacaacg atcaggaacg tcggcgcagt caattgcacg
     5461 gctatcccga gctgcgtgag cgccgcgatg ttgttgggta tgtggacgat cagctgcgaa
     5521 aggaccgccg agacctgtcg gatgcccacc cagggcgtcg gtatccatga cgatggctac
     5581 gacgaagacc agcttgccca ctatggagag cggcgcctgc tctgtcggtg ccctgtcgaa
     5641 gaagtgcttg acgccccctg ctccatccag cgctcggggc ccacggagag aaagagagga
     5701 gacggcacag ggacgtatga acaacgttac ccttcgaggg ctgatcttca acgtggactc
     5761 gtacgatccg cgcacaggat ggtccgaagc atgtagaagc ccgccgagca tcacctggac
     5821 cgacttaccc gtgtgcgaga aaaggcggcg cccctct
//
LOCUS       DBNGVD010000343         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1900811, whole genome shotgun sequence.
ACCESSION   DBNGVD010000343 DBNGVD010000000
VERSION     DBNGVD010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1900811"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="AC16CM_09165"
     CDS             complement(<1..324)
                     /locus_tag="AC16CM_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140039.1"
                     /translation="MDGRLVVFWHHGTPNTGAPPEPLFEAADRLGIRWLSYDRPGYGG
                     STSRKDRNVASAAADVSDVADALGITRFAVMGHSGGGPHALACAALLPERVLGVVSVS
                     GLAPYG"
     gene            511..720
                     /locus_tag="AC16CM_09170"
     CDS             511..720
                     /locus_tag="AC16CM_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140040.1"
                     /translation="MFQSEAREPTGRYRAEIVAGEMRQKSHQTKVQERLDEGAAKGWR
                     LVSATTTNATGSYVTGIYWDTTQER"
     gene            complement(757..1083)
                     /locus_tag="AC16CM_09175"
     CDS             complement(757..1083)
                     /locus_tag="AC16CM_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3140041.1"
                     /translation="MQGDIQNISAGQLKERLDAGDEPVVLDVRERWEFDLARIEGSRL
                     IPISELGERVTELDPAAETVVVCHHGSRSAYVTRALQRAGFESVSNLEGGLDAYSFAD
                     ESVPRY"
     gene            complement(1087..1377)
                     /locus_tag="AC16CM_09180"
     CDS             complement(1087..1377)
                     /locus_tag="AC16CM_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR3140042.1"
                     /translation="MVDHLVFFAIRDEASQEEVEDLLSSIRALRDEVPSTVDLSVGED
                     FTGRSGGYTHGIFVRFEDRDGLREYMEHPAHLAVVEKLGVTTTGRIVVDYEV"
     gene            complement(1534..>2430)
                     /locus_tag="AC16CM_09185"
     CDS             complement(1534..>2430)
                     /locus_tag="AC16CM_09185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3140043.1"
                     /translation="GDAWLGAEDERSLKALNRAVDLGLNFIDTALAYGGGHSERLVGK
                     VVAERDERIYVATKIPPKNQIWPAPDGVPVGETFPGDYVRECTERSLENLGLDALDVQ
                     QFHVWQDDWVGEGDWLEAVDDLRREGKIRSFGVSINDHQPENAIRLIETGVVDTVQVI
                     YNIFDQSPEDRLLPACEEHGVGVIVRVPFDEGSLTGRITPDTTFDEGDFRNHYFRNDR
                     KQEVQQRVQKMVADLEVSEDEIAEVALRYILSHPAVSTVIPGMRSVTNVERNTAVGDG
                     RGLPDDQVEKLKAHRWVRDFYA"
BASE COUNT          411 a          805 c          766 g          448 t
ORIGIN      
        1 cccgtatggg gccagcccag acacgctgac cacgccgagt acacgctccg gtagcagcgc
       61 ggcgcaggcg agggcatgtg gtccgccgcc ggagtgtccc atgaccgcga accgggttat
      121 gcccagcgcg tcggcaacgt cggaaacgtc ggcggcggcc gatgccacgt tgcggtcctt
      181 gcgggacgtg gagccaccgt agccgggccg gtcgtaggat agccacctga tgccgagccg
      241 gtccgcagcc tcgaacagag gctctggggg agcgccggtg ttgggggtgc catgatgcca
      301 gaagaccacg agcctgccgt ccatccctgc cgtgcccgtg tcgtaggcgt gcagcgtacg
      361 tccgtcgccg gcttctacgt tcgtctccct taccaccagc caagtctagg ggtccggatc
      421 gctgttcgca cccgcaattt tttcaatgag cgatatacgc ggtggcggcg ggtacactgc
      481 gtttcggaag gcaacccgcg aggagatttc gtgttccaga gtgaggccag ggagccaacc
      541 gggcgctacc gggcggagat cgtagccggc gagatgagac agaaaagcca ccagaccaag
      601 gtgcaggaac gcctggatga gggcgcggcg aagggatggc ggctggttag cgccaccacg
      661 acgaacgcca ccggctccta cgtcaccgga atctactggg acaccacgca ggagcggtag
      721 accacgaagc cgggcggctg gggccgtgag cccgtctcag tatctcggta cggattcgtc
      781 cgcgaaagag taggcgtcga ggccgccttc caggttcgag acgctttcga agccggcccg
      841 ctgcagggcc cgggtgacgt aggcgctcct gctcccatgg tggcagacca ccacagtttc
      901 ggcggcgggg tcgagctcgg taacacgttc tcccagctcc gatatgggga tgagccggga
      961 cccctcgata cgggccaggt cgaactccca gcgctcgcgc acgtcgagca ctacgggctc
     1021 gtctccggcg tccaaccttt ccttgagctg gccggcgctt atgttctgga tgtccccctg
     1081 caccttctag acctcgtagt cgacgacgat acggcccgtg gtggtgaccc ccagcttttc
     1141 gaccacggcg agatgcgcgg gatgctccat gtactctcgt agcccgtccc ggtcctcgaa
     1201 gcggacgaag atgccgtgag tgtagcctcc ggaacgcccc gtaaagtcct caccgaccga
     1261 aaggtcgacg gtgctcggga cctcgtcacg cagggcgcgg atcgacgaca gaaggtcctc
     1321 gacctcttcc tgcgacgcct catccctgat agcgaaaaat acgagatggt caaccataat
     1381 cgcacccttt catggccttc ctgccgcgaa aagtctagca gaagagcctg gccctaccgg
     1441 ccgcccgtcg tcgattccag gggatccctg atctgtcacc atcaggctcg gggtgcccct
     1501 cgtggtcccg ggcccgacgg gtagatcgcg cgctcaggcg tagaagtcgc gaacccagcg
     1561 gtgggctttg agcttctcga cctggtcgtc gggtagcccg cggccatctc cgacggccgt
     1621 gttccgctcc acgttggtga ccgagcgcat acccggtatc acggtcgaga cagcggggtg
     1681 gctgaggatg tagcgcaggg ctacctccgc tatctcgtcc tcggagacct cgaggtccgc
     1741 caccatcttc tgcacccgct gctggacctc ctgtttgcgg tcgttcctga agtagtggtt
     1801 tcggaagtcg ccctcgtcga aggtggtgtc gggcgtaatg cggccggtca gggagccctc
     1861 gtcgaacggg accctcacta tgacgccgac gccatgctcc tcgcaggccg gcagcagcct
     1921 atcctcaggg ctctggtcga agatgttgta gataacctgc accgtgtcga ccaccccggt
     1981 ctcgatgagc ctgatggcgt tctcgggctg atggtcgttt atggagacgc cgaaggagcg
     2041 gatcttgcct tcccgcctca ggtcgtcgac cgcctcaagc cagtctcctt caccgaccca
     2101 gtcgtcctgc cacacgtgga actgctgcac atccagggcg tcgagcccga ggttttcgag
     2161 gcttctttcg gtgcactccc tgacataatc cccagggaac gtctccccca ccggaacgcc
     2221 gtcgggagcc ggccagatct ggtttttcgg cgggatcttc gtggcgacgt aaatccgctc
     2281 gtcccgctcc gccacgacct ttccgactag cctctcgctg tgtcctccgc cgtaggccag
     2341 ggccgtatct ataaagttga ggccgaggtc taccgccctg ttcagggcct tgagtgacct
     2401 ctcgtcctcg gcccccagcc aggcatcgcc
//
LOCUS       DBNGVD010000344         1835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1719040, whole genome shotgun sequence.
ACCESSION   DBNGVD010000344 DBNGVD010000000
VERSION     DBNGVD010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1719040"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="AC16CM_09190"
     CDS             <1..1184
                     /locus_tag="AC16CM_09190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3140044.1"
                     /translation="EPQNRGQEGLERRNHELSVLNEIARELNRSVELGEALGFTLSQV
                     AELLGLKTAWIWLMEESSPEPYLAAARNLPPALADDPRRMDGSGYCYCLDTYRRGDLE
                     GAANVNVLTCSRLDGLVDGTGGLRYHASIPLYAGEKKLGVMNVASPQWRGLSPEDLQL
                     LSTIGDLLAIAVERARLFDRSARLGAVEERNRLAREIHDTLAQGLTATGLQLESAEAL
                     LDHGAAAERAREPLRRALTLTRSNLDEARRSVLDLRAAPLEGRSLPEALKTLVDRWEA
                     ETGVNTRFKAINGSHPLPPRVEVALFRICQEALTNVARHAEAGRVTVRLVATPDEVRL
                     VVMDDGRGFDPSRVTAERHGVVGMKERAHTVGGRLQFQSETNEGTRLEVAIPLGGAQD
                     G"
     gene            1177..1821
                     /locus_tag="AC16CM_09195"
     CDS             1177..1821
                     /locus_tag="AC16CM_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140045.1"
                     /translation="MDSIRLLVADDHPMLREGLVAVLGTQPDFEVVGEAADGDEVVRQ
                     AGSLDPDVILLDLEMPGTDGVEALEQLRDAGSRARTIVFTAYNTDERILGSLRAGARG
                     YLLKGASRQEIFGAIRTVQAGGSLLEPAVTDRLLNRIDGEENVPENRRLTPRELEVLA
                     LLARGLKNAEIADQLYISERTVKFHVGSILTKLQAQNRTEAARIAARRGLIDDS"
BASE COUNT          336 a          622 c          614 g          263 t
ORIGIN      
        1 ctgaaccgca gaaccgggga caggagggcc tggagagacg caaccacgag ctctcggtgc
       61 tcaacgagat cgcgcgcgag ctcaaccgct ccgtggagct cggtgaggcg ctcgggttca
      121 cgctctcgca ggtggccgag ttgctcgggt tgaagacggc ctggatctgg ctcatggaag
      181 aatcctcgcc cgaaccttac ctcgccgccg cacgaaacct gcccccagcc cttgccgacg
      241 acccgcggcg tatggacggt tcaggatact gctactgcct ggatacgtac aggaggggcg
      301 acctggaggg cgcggccaac gtgaacgtgc tgacctgcag ccgcctcgac gggctcgtgg
      361 acggcacggg aggactacgc taccacgcca gcatcccgct ctacgccggg gagaagaagc
      421 tcggcgtgat gaacgtggct agcccgcaat ggagggggct ctcgcccgag gacctgcagc
      481 ttctctccac cataggggat ctgctcgcca tcgctgtgga gcgggcacgc ctcttcgacc
      541 gaagcgccag gcttggcgcc gtcgaagagc gtaaccggct ggcgcgtgag atccacgaca
      601 ccctggccca gggcctgacc gccacgggcc tccagctgga gtcggcggag gcactcctcg
      661 atcacggggc cgccgcggag cgcgcccggg aaccgctcag gcgcgccctc accctcacca
      721 ggtccaacct cgacgaggcc aggcgttccg tgctcgacct gagggccgcc ccgctggagg
      781 gccgctcgtt gcccgaggcg ctcaagacgc tcgtggaccg ctgggaggcg gagacgggcg
      841 tgaacacgag gttcaaggcc atcaacggca gccacccact tccgccgcgg gtcgaggtag
      901 ccctcttccg tatctgccag gaggcgctca ccaacgtcgc ccgccacgcc gaggcgggcc
      961 gggtaacggt ccggctcgtg gccacgccag atgaggtgcg gctcgtggtc atggacgacg
     1021 gccgcggttt cgatccctct cgcgtaacgg cagagcgtca cggcgtcgtc ggcatgaagg
     1081 agagggctca tacggtcggc ggccgcctcc aattccagag cgaaacgaac gagggaacgc
     1141 gcctcgaggt cgcgatcccg ctagggggag cccaggatgg atagcatccg gctactcgtc
     1201 gccgacgacc acccgatgct gcgcgagggc ctcgtcgccg ttctgggtac ccaacccgac
     1261 ttcgaggtgg tcggggaggc cgccgacggt gacgaggtgg tcaggcaggc cggaagcctg
     1321 gaccccgacg ttattctgct agacctggag atgcctggga cggacggcgt cgaggccctt
     1381 gaacagctgc gcgacgcggg atcgcgcgca aggaccatag tcttcacggc ctacaatacc
     1441 gacgagagga tcctgggctc cctgcgcgcc ggcgcccgcg gctacctgct caagggcgcc
     1501 tccaggcagg agatcttcgg cgccatccgt accgtgcagg caggtggctc gctgctggag
     1561 ccggccgtca cagacagact cctgaaccgc atagacggcg aggagaacgt tccggaaaac
     1621 cgacgcctca cgcccaggga gctggaggtg ctcgccctcc tcgcaagggg cctgaaaaac
     1681 gccgagatag ccgaccagct ctacatctcg gagcgaacgg tcaagttcca cgtcggctcc
     1741 atccttacca agctgcaagc ccagaacagg accgaagccg cccgaatagc cgcccgccgc
     1801 ggcctgatag acgattctta gagctatcag ctttt
//
LOCUS       DBNGVD010000345         3585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3176997, whole genome shotgun sequence.
ACCESSION   DBNGVD010000345 DBNGVD010000000
VERSION     DBNGVD010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3176997"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC16CM_09200"
     CDS             <1..296
                     /locus_tag="AC16CM_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3140046.1"
                     /translation="LTPEELRDLAERRHVVASHTRTHVSASPGALTGLQPVTLEREAS
                     ASKHDLEEIVGKPVGALAWLEGAALGSNAAADRAVARAGYELLFANHAVQSVS"
     gene            375..2186
                     /locus_tag="AC16CM_09205"
     CDS             375..2186
                     /locus_tag="AC16CM_09205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR3140047.1"
                     /translation="MTNVPRPEYPRPQFYREGWTNLNGQWRFAFDDDNVGLAERWQDA
                     TREGLDSGTSPFDRVITVPFCFQSELSGIGETDFHDVVWYARTFEYTPGDDERLLLNF
                     GAVDYRATVWVNGMQVASHEGGHTPFSADVTDVLAGEVGVNVLIVRAEDPSRDVTIPR
                     GKQYWKEESEGIFYTRTTGIWQTVWLEPVNRRRIAGLRLTPDVDAASIGLDVEIEGVE
                     RGMSLRVGIAFRGAPVLEDTMGLWSSRVERSLPLLRRGAAPETAHLSAWPSVALWSPE
                     EPNLYDLELELLDGNGAVVDTVESYFGMRKIESRDGRVLLNGRPLYQRLVLDQGYFPE
                     GILTAPTDDALRRDVELAREMGINGARKHQKVEDPRWLYWADSLGLLVWGEMANAYQY
                     SPDYVGRITTEWQEALARDYNHPSIVAWVPMNESWGVPNLAHDPTQRHHLLALYHLTR
                     SLDATRLVVSNDGWEHALTDLCAIHDYRDAEALRESYATPGSAVAATPSGRPIYVPGH
                     SYGGEPVLITEFGGIAFAGAEAGWGYSTVSDAEEFLARYGSLIKALQECETVQGFCYT
                     QLTDVEQEVNGLLTYDRKPKASLDAIRKLTTSREQSR"
     gene            2588..>3585
                     /locus_tag="AC16CM_09210"
     CDS             2588..>3585
                     /locus_tag="AC16CM_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3140048.1"
                     /translation="MESDFRRNHSGIQLDSTTLAWGAPYYTKLSMSTVGGRPPEVAIL
                     HQTHLASYAPAGLLEPLDPGLLSDFGIGPENFLPEILEASKYQGEIYTIPLDTHPFVQ
                     YYNTDICKKAGLLGADGNLVPLEGPDAVIDALKKAKEVTGDLGLTIYAADPAGPWRLF
                     YTLYGQLGGAVLSDDATEVILDEDKVVQAAEFMIALAQDAKVTAANVDYGGSVAQFQS
                     GTAGFQWNGDWEVTTYLDADLPFSMTPFPNVFGENNISQADRHTFVIPRQPKNDPASM
                     EASLEFVSSMLKNSFTWAEGGHIPAYLPITRTEQYKKLSPQSNYVDVADNVVLDPKA"
BASE COUNT          700 a         1154 c         1122 g          609 t
ORIGIN      
        1 ccctcacgcc cgaagagctg cgcgatctcg cggagcgcag gcacgtcgtc gcctcccaca
       61 cgcggaccca tgtgagcgcc tctcccgggg cgctcacagg cctccagccc gttaccctgg
      121 aacgcgaagc gtcagcctcg aagcacgatc tcgaagagat agtgggtaag ccggtcggcg
      181 ctctcgcgtg gttggaaggc gcggcgctgg ggtccaacgc tgccgcggac cgagcggttg
      241 cgcgggcagg ctacgagctt ctgtttgcca accacgccgt ccagagtgtg tcgtagcgag
      301 ggccgatctg ggcccctcgg tttgctgtat attcctacga gcccagcacc agtacgaccc
      361 gtgggaggaa acgcatgacg aacgtgccgc gccccgagta cccgcgcccg cagttctatc
      421 gggagggttg gaccaacctg aacggccagt ggcgcttcgc tttcgacgac gataacgtgg
      481 gcctggccga acgctggcaa gacgccaccc gggaaggtct cgactcgggc acgtcgccgt
      541 tcgaccgcgt catcacggtg cccttctgtt tccagtcgga gctctccggc atcggcgaga
      601 cggacttcca cgacgtagtc tggtacgccc gcaccttcga gtacacacca ggggatgacg
      661 agcgtcttct cctcaacttc ggggccgtgg attaccgcgc gactgtctgg gtgaacggca
      721 tgcaggtggc atcccacgaa ggcggacaca cgccgttctc ggcggacgtg acggacgtgc
      781 tggccggcga agtaggggtc aacgtcctga tcgtgcgcgc cgaggatccc agtagggacg
      841 tgacgatccc gaggggcaaa caatactgga aggaagagtc ggagggcatc ttctacacga
      901 ggaccaccgg catctggcag accgtctggc tggagcccgt aaaccgccga cgtatagcgg
      961 ggctacgcct taccccggac gtggacgccg cgagcatagg cctcgacgtc gagatcgagg
     1021 gtgtcgagcg cgggatgagc cttcgggttg gtatcgcctt cagaggagcg ccggtgctcg
     1081 aagacaccat gggcctgtgg tcttcgcggg tcgagaggag cctgccgctt ctccgccgcg
     1141 gcgcagcccc cgagacagcc cacctctcgg cctggccgtc cgtggccctc tggagccccg
     1201 aagaacctaa cctctacgac ctcgagctcg agctcctcga cggaaacggc gcggtggtgg
     1261 acacggtaga gagctacttt gggatgcgca agatagaatc cagggacggc agggtcctcc
     1321 ttaacggtcg tcccctctac cagaggctcg tgctcgacca gggctacttt cccgagggca
     1381 tcctgaccgc acccaccgat gacgccctgc gccgcgacgt ggagcttgcc agggagatgg
     1441 gtatcaacgg tgcccgcaag caccagaagg tcgaggatcc ccgctggctg tactgggctg
     1501 actcccttgg cctgctggtc tggggcgaga tggccaacgc ctaccagtac agcccggatt
     1561 acgtcggacg aataacgacc gagtggcagg aggcgctcgc gcgcgactac aaccacccgt
     1621 ctatcgtggc ctgggtcccg atgaacgaga gctggggcgt cccgaacctg gcacacgacc
     1681 ccacccagag acatcacctc ctcgccctct accacctgac gcgctccctc gacgccacca
     1741 ggctcgtcgt gtcaaacgac ggctgggagc atgccttgac cgacctgtgc gccatccacg
     1801 actaccgcga cgccgaggcc ctgagagaga gctacgcgac gccaggttcg gccgtcgcgg
     1861 ccaccccttc tggacgcccc atctacgttc caggccactc ctatgggggg gagccggttc
     1921 tgatcaccga gttcggcggt atcgccttcg ccggcgccga agcgggctgg ggctacagca
     1981 cggtctccga tgcagaagag ttcctggcac gctatgggtc cctgatcaaa gccctccaag
     2041 agtgcgagac cgtccagggt ttctgctaca cccagctgac ggacgtcgag caggaggtca
     2101 acggtctgct tacctacgac cgaaaaccga aagcgagcct cgatgccatc cgcaagctca
     2161 ccacctcacg agagcagtcg cgctagacga ccgcgaggat tcaccccaac ctcagagcgg
     2221 ctcggagcgg gaatcgcccg cttcgaagag agccacaaga atctagttta tagctggggg
     2281 ctcgccctcc ccgacccagg cccccgcaaa ccaccctggc tgttgacccg ggtcagaaga
     2341 agtgctacaa aggtgtctag gcgaacgttc gctttagggg aagtgaggag ccatgaccga
     2401 gaggttagaa agaccggatg gccggggcct ggggcggatc agccgtcggc gatttctcgg
     2461 cgggtcgctc gcggcattgg ggggattgac tctgtcgggg tgcggcgttg catcatttgc
     2521 gggaacgagt cggacggtag atttctggaa tctctttggc gggggagacg gagcgcggct
     2581 cggagagatg gagagcgact ttcgcaggaa ccactccggg atacagctcg actcaacgac
     2641 gttagcctgg ggtgctccat actacaccaa gctctcgatg tccacggtcg ggggaaggcc
     2701 gcccgaggtg gccatcctac atcagactca ccttgcctct tatgcgccag cggggttact
     2761 cgagcccctc gacccggggt tgctctcgga tttcggaatc ggccccgaga acttcctgcc
     2821 cgaaatactc gaggcatcca agtaccaggg tgagatctac accattccgc tagacaccca
     2881 tccgttcgtc cagtactaca acacggatat ctgcaagaag gccggtcttc tgggagcaga
     2941 cggcaatctc gtccccctgg agggtcctga cgcggtcatc gacgcgctca aaaaagccaa
     3001 agaggtgacc ggagatctcg ggctcactat ctacgccgcg gatccggctg gtccgtggcg
     3061 cttgttctac accctctacg gacagctcgg tggggccgtc ctctcggatg acgccaccga
     3121 ggtcatcctg gacgaggaca aagtagttca ggccgcggag ttcatgatcg cgctcgcgca
     3181 ggatgccaag gtcacggcgg ctaacgtcga ctacgggggt tcggtggcgc agttccagag
     3241 tggaactgcg ggcttccaat ggaacgggga ctgggaggta accacctacc tggatgcgga
     3301 tctgcccttc agcatgacgc ccttcccaaa cgtattcggc gaaaacaata tctcgcaggc
     3361 cgacaggcac accttcgtca ttccaaggca gccgaagaat gatcccgcca gtatggaggc
     3421 ctcgctcgag ttcgtcagct cgatgctcaa gaacagcttc acctgggccg agggcggcca
     3481 tatcccggcc tatctcccga ttaccagaac cgagcagtac aagaagctca gcccccagtc
     3541 caactacgtg gatgtcgctg acaacgtcgt gctcgacccc aaggc
//
LOCUS       DBNGVD010000346         3854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2811717, whole genome shotgun sequence.
ACCESSION   DBNGVD010000346 DBNGVD010000000
VERSION     DBNGVD010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2811717"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="AC16CM_09215"
     CDS             <1..521
                     /locus_tag="AC16CM_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140049.1"
                     /translation="LVRMCQLALPGMRRQGFGRIVNLSSMGGRLTFPGAGFYHASKYA
                     VEAVSDALRYEVAGFGIKVSVIEPGLIRTGFADTAVGSIGGQDDGPYAGFDEAVARVT
                     TENYERGPVSRLAGEPEAVAEAIEQAISASNPRSRYAVTPSAHLFIGLRRLLPDGAWD
                     AALRTTYPQPGR"
     gene            525..1265
                     /locus_tag="AC16CM_09220"
     CDS             525..1265
                     /locus_tag="AC16CM_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140050.1"
                     /translation="MQIEGTTFLVAGGGSGLGAATTDMLAASGARVVVADLKGEAPEN
                     GRFVETDVTDEGSVRSAVDEARGLGEFRGAVNCAGVASAEKVLGREGPHSLDSFSKVV
                     QVNLVGTFNVIRFAAEAISRNEALESGERGVIVNTASVAAFDGQIGQVAYAASKGGVV
                     SLTLPVARELAGSGIRIMTVAPGIFDTPMMAGLPENARVSLGEQVPFPSRLGKPEEYA
                     ALVKHIIENEMLNGEVIRLDGAIRMAPK"
     gene            complement(1346..1876)
                     /locus_tag="AC16CM_09225"
     CDS             complement(1346..1876)
                     /locus_tag="AC16CM_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3140051.1"
                     /translation="MAKRGEVLENPITGQRIIFRRTPGDTAGGLLEVESVYTTPSPSR
                     PPTHYHPSQEEIFRVLAGELHVVAYGGHRVLKKGETLVIVEGSRHQMWAEEAGVRVNW
                     QTRPALETAAFFETVFGLASEGKTDEKGVPNLLQSAVIARKYADVFRLASPPWPIQKL
                     LFAVLAPLGKILGYKS"
     gene            complement(2015..2446)
                     /locus_tag="AC16CM_09230"
     CDS             complement(2015..2446)
                     /locus_tag="AC16CM_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140052.1"
                     /translation="MWAEIEKYLPLFESAVLNTADGEGYPYSIRCRPELDRPVEIFRI
                     DLPGETDVQAGPASLLFHNHDEELWNQKIFLLRGRLEESDDGHTFTPEKFVPSIGTGG
                     LLGTVHMIRSVRRASAGYLGRRNLVRPRIPWDEIEALKKEA"
     gene            complement(2436..2984)
                     /locus_tag="AC16CM_09235"
     CDS             complement(2436..2984)
                     /locus_tag="AC16CM_09235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3140053.1"
                     /translation="MPGHREEGAQDVAPSGGRFALPPKVEEVFREFRTCEFSTLARDG
                     TPITWPLITLWQPEEQRFVLTTSIGLPNKAFNARRDGRVSLLFSDPTASRLDDPPAVL
                     VQGDAVVPEVIHTSPYDLEEYWQRTWRMQPAGKAYGSNFLTRSPMDWYYMRLYIYVRP
                     RRILVWPGGDFAKQPEEVDHVG"
     gene            complement(3024..3629)
                     /locus_tag="AC16CM_09240"
     CDS             complement(3024..3629)
                     /locus_tag="AC16CM_09240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140054.1"
                     /translation="MDGLEIGPLDPSDIEEVVGVLARGMLDNPVHVAAFGEDPEIRHQ
                     RLRILMSAAFRARDLSHAFVARDEEGAVVGVCGMMSPGGCRPDLKQQLSLLPTLLRLG
                     PRPAARVMSWLGVWGEHDPEERHWHLGPLAVDAHLQGMGVGGRLMQVFCAQTDAARDD
                     AYLETDKAINVGFYERFGFRVVGEADVLGVKNYFMHRKGAR"
BASE COUNT          652 a         1242 c         1237 g          723 t
ORIGIN      
        1 ggctggtgcg catgtgccag ctagcgctgc ccgggatgcg gaggcaggga ttcggcagga
       61 tcgtcaacct ctcctccatg ggcggaaggc tcaccttccc cggcgctggc ttctaccacg
      121 cttccaaata cgccgtcgag gccgtaagcg acgccttgcg gtacgaggtg gcgggtttcg
      181 gcatcaaggt ctccgtcatc gaacccggcc tgatccggac cggtttcgcg gacaccgccg
      241 tgggctcgat aggcgggcag gacgatggtc cttacgccgg cttcgacgag gccgtcgcga
      301 gggtgacgac agagaactac gagcgaggcc ccgtctccag gctggcagga gaacccgaag
      361 ccgtcgccga ggccatagag caggcgatct ccgccagcaa cccccgaagc cgctacgccg
      421 ttacaccttc cgcgcaccta tttatcgggc tcagacgtct cctgcccgac ggtgcgtggg
      481 atgccgcgtt gaggacgacc tatccacaac cagggaggta gagggtgcag atagagggga
      541 ccacgttcct cgtggccgga ggtggctcgg gcctcggcgc cgcgaccacc gacatgctcg
      601 ccgcctcggg cgcccgcgtg gtcgtcgccg acctgaaagg tgaggccccg gagaacggcc
      661 gcttcgtcga gaccgacgtt accgacgagg ggagcgtgag gtcggccgtc gatgaggccc
      721 gggggcttgg agagtttcgc ggcgccgtaa actgcgccgg ggtcgccagc gccgagaagg
      781 tgctagggag ggagggcccg cattcgctcg actctttctc gaaggtcgtg caggtcaacc
      841 tcgtcggcac cttcaacgtc atcaggttcg cggctgaggc tatatctcgg aacgaggcgc
      901 tggagagcgg ggagcggggc gtaatcgtca acacggccag cgtggcggcg ttcgacggac
      961 agatcgggca ggtcgcctac gccgcgtcga agggtggcgt cgtctccctt acgctgcccg
     1021 tggcccggga gctcgccggc agcggcatcc gcatcatgac agtagcaccg ggtatcttcg
     1081 acacccccat gatggccggc ctcccagaaa acgccagggt atcactggga gaacaggtac
     1141 ccttcccctc ccgactgggc aagcccgagg aatacgccgc gctggtcaaa cacatcatcg
     1201 agaacgagat gctcaacggc gaggtcataa ggctggacgg cgccatccgg atggcaccaa
     1261 agtaggtttc aggcatcagg agtagagtgc tcctgatgcc tgtggccttc aatcacccgg
     1321 ggttaggtct ttccgaagat ccgtttcagg acttgtagcc caggattttg cccaacgggg
     1381 cgagtaccgc gaagagtagc ttttggatcg gccatggcgg gctggctaga cggaacacgt
     1441 ccgcgtactt cctggcgatg accgctgact gcaggaggtt ggggacgccc ttttcgtctg
     1501 tcttcccttc cgaagcgagg ccgaacacgg tctcgaagaa ggccgccgtc tccagcgccg
     1561 gacgggtctg ccagttgacg cgcacaccgg cctcttcggc ccacatctgg tgcctggagc
     1621 cctccacgat gacgagcgtc tcgcccttct tcaggaccct atgcccgccg taggcaacga
     1681 cgtggagctc accggccaga acccggaaga tctcctcctg cgagggatgg tagtgggtgg
     1741 gcggcctgga aggcgacggg gtcgtgtaga ccgactcgac ctccagcagc ccgccggccg
     1801 tgtcccctgg ggtccggcgg aagatgatcc gttgcccggt tatggggttt tcgagcacct
     1861 cgcctctctt cgccatgcct ccgcctttct ggttttgggg ctgatagctg gtcactccgg
     1921 cgtattttat gcgcgctttc gcaacttctc cgaagcgtcg gtcggtgaag gttccgcttt
     1981 gcggaattcg ggaggaggtt ggcttacctg gtggtcacgc ctccttcttc agggcttcga
     2041 tctcgtccca cgggatgcga ggacggacca ggttgcgtct gcccaggtac cctgccgagg
     2101 ccctgcgtac gctgcgaatc atgtgcaccg tgccgaggag tccccccgtt cctatgctcg
     2161 gaacgaactt ctcgggcgtg aaggtgtggc cgtcatccga ctcttcgagc cttcccctga
     2221 ggagaaatat cttctggttc cacagctctt cgtcgtggtt gtgaaagagc aggctggcgg
     2281 ggccggcctg aacgtccgtc tcccccggga ggtcgatcct gaagatctcc accgggcggt
     2341 ccagctcggg tcggcagcgg atgctgtagg ggtacccttc gccgtcagcg gtgtttagga
     2401 cggcgctctc gaacagcgga aggtacttct cgatctcagc ccacatggtc gacctcctct
     2461 ggctgcttcg cgaagtcgcc gcctggccag accagaatcc ggcgcggcct aacgtagatg
     2521 tagagcctca tgtagtacca gtccatggga gaacgcgtca gaaagttgga gccgtacgcc
     2581 ttgcccgcag gctgcatccg ccaggtcctc tgccagtact cttccaggtc gtacgggctc
     2641 gtgtggatca cctcgggcac cacagcatcg ccctgtacga gcaccgccgg gggatcgtct
     2701 agcctgctcg cggtgggatc ggagaacagc agcgagaccc tgccgtcgcg ccgcgcgttg
     2761 aaggccttgt tcggaagtcc gatcgaggtg gtcagcacga accgctgttc ctccggctgc
     2821 cacagcgtta tgagaggcca ggtgatcggc gtgccgtcgc gggcgagggt ggagaactcg
     2881 caggttcgaa actccctgaa gacctcttcc accttcggtg gcagggcgaa cctgccgccg
     2941 gacggcgcca cgtcctgtgc accttcttct ctatgcccgg gcatcgagcc tctcctctcc
     3001 tcaggtgtgc tcacgtcgcg catctatctc gctcccttcc tgtgcatgaa gtagtttttc
     3061 acaccgagca catccgcttc tccgaccacc ctgaaaccga agcgctcgta gaagccgacg
     3121 ttgatggcct tgtccgtctc caggtaagcg tcgtcgcgcg cggcgtccgt ctgggcgcag
     3181 aagacctgca tgaggcgtcc ccccacgccc atgccctgca ggtgcgcgtc gacggccagc
     3241 ggcccgaggt gccagtggcg ctcctcgggg tcatgttcgc cccacacgcc gagccaactc
     3301 atcacgcgcg cggcggggcg gggacccaga cggagcagtg taggcagcag cgacaactgc
     3361 tgtttcaggt ctgggcggca ccctcccggg gacatcatgc cgcatacccc gacaacggcg
     3421 ccctcttcgt cgcgcgccac gaaggcgtgc gacaggtccc tggcacgaaa cgccgccgac
     3481 atcaggatac ggagcctctg gtgcctgatc tccgggtctt ccccgaaggc tgcgacgtgt
     3541 acggggttgt cgagcatgcc gcgcgcgagt acgccaacga cctcttcgat atccgagggg
     3601 tcgagcggac cgatctccaa cccgtccaac catgtgcctt gtccggtaat actgtgcatt
     3661 gccttctcct ctcgtttacc gtgcctggat tatggtcgtg gcgggtggcc ttctgccatc
     3721 cacaaagccc ccacacttcc cccacagtga agatgtgtgg gagcagatcc cgggcgcggc
     3781 tctcccatac cgttgtgtac cgggatttcg gatgctggta agcttgcaaa ctatggagag
     3841 agaaggcggc gcac
//
LOCUS       DBNGVD010000347        11194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3177719, whole genome shotgun sequence.
ACCESSION   DBNGVD010000347 DBNGVD010000000
VERSION     DBNGVD010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3177719"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1871
                     /locus_tag="AC16CM_09245"
     CDS             297..1871
                     /locus_tag="AC16CM_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3140055.1"
                     /translation="MQSERLGEDTTAGKIEGLEKLRDAAAHPAPETAVERQRERGKLT
                     ARERVELLLDGGTFVELDRYRVHRSHNFGLEDNKPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVYAEKICKVMDLALSTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVIPQISVVVGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEDVTF
                     EDLGGAATHNTRSGVAHFSAPDEEICFDDVRYLLSFLPENNLESAPYFRPTDNPERKD
                     EDLAALIPDSPQRPYDIRGAIGGVVDDGEFFEVQEGWAQNLVVGFARLDGRAIGVVGN
                     QPSVLAGTLDIDASVKGARFVRFCDAFNIPLLTFVDVPGFMPGTDQEWGGIIRHGAKL
                     LYAFSEATVPKMTVITRKAYGGAYDVMCSKHIGADVNVSWPTGEIAVMGAPAAVGIIF
                     RREISTAEDPEARRTELIESYEQQFNNPMVAAEMGFIDDVIDPRETRPYLIKALQMIR
                     TKRPDRPPRKHGNIPL"
     gene            2105..3850
                     /locus_tag="AC16CM_09250"
     CDS             2105..3850
                     /locus_tag="AC16CM_09250"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3140056.1"
                     /translation="MFGKVLIANRGEICVRIVRALREMGISSVAVYSDADRDALHARI
                     ADEAYHVGPTLASESYLNVGRLIEVARESGAEAVHPGYGFLAESAPFARAVEEAGLVW
                     IGPHPGAIEAMGSKVESRRIMAQANVPMVPGTEDPIAAAKEVRLFGEEHGFPVAVKAS
                     AGGGGKGFAVAVDHSEAENAFARASREGEAYFGDGSVYVEKYLRAPRHVEIQVVRDKL
                     GNAAHLGERDCSIQRRHQKLLEECPSPAIDQRMREDMGRAALAAAEAVDYDNVGTVEF
                     LVENGENGDGFYFLEMNTRVQVEHPVTEEVTGIDIVKTGIRIAAGEPLPFSQRDVAWR
                     GHAIEVRLNAEDPARDFAPSPGAVTVYEEPGGPGVRVDSSLRGPGLVAESYDPLFAKL
                     IVRGGDREEALARLGRALAEFGVEGIYTTLPFFRAILSDEAFVSGDYSTGFVAERMGL
                     LEIEPTPPAEPATDRETSTREVEVEVNGKLFRVKVFGEENSHGPAGPPRRRQGETRRA
                     SASGGAIAAPMQGTIVRVLVEVGQEVEADEPVCILEAMKMESEVRSQKAGTVSEVRVE
                     SGQTVRSGDPLVLVE"
     gene            3860..4933
                     /locus_tag="AC16CM_09255"
     CDS             3860..4933
                     /locus_tag="AC16CM_09255"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3140057.1"
                     /translation="MAEHAAEYEWHGEGEEAEIFLYAPDDAVFERVLPAARLPGVQSP
                     VYAAASPEGFGWATTSSSHVAPDLLSLPARGLLLVAGTRSENLREDARELANRIQRDL
                     PEVGSRLPSLNEAGVGRLCGSGASAAAEDGLIEEDDLDFLDALPGDADALGRRALSAG
                     PRDWQEAAVPELRVVSEVFDTEGADSLGLEPGSLAISIRVGGGDLGWLALSAHAERLR
                     AGPEDFGEGRDLPSAPTESGEAQDLLAALYASSNFADARAARTVWALRRLLDVATGGL
                     EIRATWRMGGVEERDGLLVHRRALTLLGEGDALVCGRYAAFGTGNMSSSAPPFGIPEA
                     EDGWPWEEAGLVERLAALDPPEG"
     gene            4936..5214
                     /locus_tag="AC16CM_09260"
     CDS             4936..5214
                     /locus_tag="AC16CM_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140058.1"
                     /translation="MDLLMVRDRNTGRFVYTERLERRAGETSWEYVRRSVRRESRIRT
                     RFHGGTMQVIVGWNVDSVEEFLRSHPEYRIDGVSQSEGRDDSSKGDGV"
     gene            5267..5440
                     /locus_tag="AC16CM_09265"
     CDS             5267..5440
                     /locus_tag="AC16CM_09265"
                     /inference="COORDINATES: protein motif:HMM:NF039182.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HIR3140059.1"
                     /translation="MAALAWNTAIQNLFDRIFGKAGAALAGQVLYATIAVSRAAERAK
                     KQEAEQKTETNPG"
     gene            complement(5650..6150)
                     /locus_tag="AC16CM_09270"
     CDS             complement(5650..6150)
                     /locus_tag="AC16CM_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3140060.1"
                     /translation="MSADGFTKMSIYGMNMDLFSSSPIVILKVEDENRYLPIWIGQAE
                     ARSILFKLQNQEFVRPLTHDLALNLVNELGGSMERITVTELKDQTFFATIRLEIEGKT
                     VEVDSRPSDAIALAIRSGAEIFASDDVIEEAGVVFEEAMEEAPEEEVVDKFKDWMNRV
                     SPEDFK"
     gene            6308..6381
                     /locus_tag="AC16CM_09275"
     tRNA            6308..6381
                     /locus_tag="AC16CM_09275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6342..6344,aa:Ile,seq:gat)
     gene            complement(6576..7478)
                     /locus_tag="AC16CM_09280"
     CDS             complement(6576..7478)
                     /locus_tag="AC16CM_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140061.1"
                     /translation="MGTTRMLSKRGDGPPVYTYDVMPGVPPVSAMRLDRGFSELRPGA
                     HSHDFLLLSYFERGGGSFRLGEREWRVEAGDAYLIAPGEVADASGLVAAEGWAVFFPP
                     EVLGPEAPGAFLSWRAHPLLFPFVRGAMEGVQNLKVPVAKRSSWSERLSALNLELRRK
                     TDGYREAVLAHLTLLLVDIGRLASDVVGDLRLKEEPLLAEVFGFIEKRYNEHISLEDV
                     AKAVSLSPGYLTTVVRRKTGRTVLEWIVERRMVEARRLLVQTDLAVEEVSRRVGYGDA
                     GYFVRSFRRIHSVTPLGWRRSGRP"
     gene            7731..8093
                     /locus_tag="AC16CM_09285"
     CDS             7731..8093
                     /locus_tag="AC16CM_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3140062.1"
                     /translation="MDAIGARDLVDRYFSALTRRDWTTVRTLLHDDLFFEGPLATLGT
                     ADEYLQGLEHFTARMTGAERRIVFAEGEDVLQIYEVSFDAPAVTAPVAEWLRIRDDRI
                     ATIQMLLDPRPFSGAQPS"
     gene            8262..9731
                     /locus_tag="AC16CM_09290"
     CDS             8262..9731
                     /locus_tag="AC16CM_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140063.1"
                     /translation="MHGHGASHQHARNAPGYAGTPPGTRQWIALILLCFAQFMLIVDI
                     TVVQVALPSIGEVLGLGRESLTWVVTAYTLFFGGLMVFGGRLADALGARRTLLAGLAL
                     FTLASLLCGLATTGAALVAGRAAQGVGAALLSPSALAIITTTFTGDHRSKALGIWAAI
                     GGVGAALGVMLGGILTSGIGWQWVFFVNVPAGLLILLILPTVVGRGVSTSVRRQVDVP
                     GALLVTVATALLIYGLINAGDAGWGAGGTLISLGAAAAAYLAFVVVERVASEPLMRAE
                     TLARRAVISGAFVMLVATGLMFALFFLSSFYLQRILGFNALETGLTFLPAAIAITIGA
                     QLAGHFIGRLGGRTVATVGFLLTALGIGLMTQISPQEGAFTTLLPGFLIAALGIGPAF
                     VAATTTALADVPYEEAGVASGLVNTFHELGGSIGVAAVSTVAAASFAPSATGVGGFTT
                     ALVAAAVGAGAAAVVVLILVPRGEPTDTVVGHGHETGAR"
     gene            9731..10822
                     /locus_tag="AC16CM_09295"
     CDS             9731..10822
                     /locus_tag="AC16CM_09295"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3140064.1"
                     /translation="MMERTAIVVVGGYGAVGRTVCTELATRFPGHVFAAGRNLEKASS
                     FSQETRGRVLPLELDITDPSATAKRLDGAGVVVACAESGDATRDAGLARAYLDRGIHY
                     VDVSASYALLNEVEKLDALARRSAVTAVLSVGLAPGLTNLLARQCVDVLGDELRNIDV
                     SILIGLGEAHGKAAVRWIVENLDKRFELPGFGTVGSLEDPKKTFFSVPYGERTCYRFD
                     FADQHVLARTLGAEQVATRLCFDSAVATHLLALAKQVGVFRALGGPRGQDFLVALFSR
                     LRVGSDGFEVKAEAEGREGGIYSCSARGRGEARTTGIVAAFVVERLLTSPSLERGVFH
                     IEQLFEPVELFGRMKELGLKVNLKNGRSE"
     gene            11000..>11194
                     /locus_tag="AC16CM_09300"
     CDS             11000..>11194
                     /locus_tag="AC16CM_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140065.1"
                     /translation="MNAYSKDLRLRVLGAVDRGLPREEVSRLFGISRSTIKRWVKRRR
                     EGEDLEPRRSTGRKRRILSTA"
BASE COUNT         1983 a         3573 c         3627 g         2011 t
ORIGIN      
        1 agaagacgag ctgctcgtag cggtactccg ccagcttctc gaagacttcc attcgcctct
       61 cctttccccc atccccggtt tttcgtccgg gattacattc tactatcccc gccacttccg
      121 gctcccccga gcggtctttc gcagggtgag tctggagtgc gtgaaggcaa caagcaacag
      181 tcatcaggac ttttcctgaa gcctgaaacc cgtagcctga agcctcctct ccagcggcac
      241 cttcttttcc agaatcgcca taaaggggaa cttcgtgcgt gctagaatgc gcctctatgc
      301 aatccgagag gcttggcgag gacaccaccg ccggaaagat cgaggggctt gagaagctgc
      361 gggatgccgc ggcgcacccg gccccggaga cggctgtgga gcggcagcgc gagaggggca
      421 agctgacagc ccgtgagcgc gtcgagctgc tgcttgatgg gggcaccttc gtggagttgg
      481 accgctacag ggttcaccgc tcccacaatt tcgggctcga ggacaacaag ccgctgggtg
      541 atggggtcat caccggctac ggcgaggtct cagggcgcaa ggtctgcgtc ttcagccagg
      601 acttcacggt cttcggcggc tcgctcgggg aggtgtacgc ggagaagatc tgcaaggtta
      661 tggacctcgc gctctctacg ggatgcccca tcatcggcat caacgactct ggaggggcca
      721 ggatccagga gggcgtcgtc tccctggccg gctacgccga catctttcac cgcaacgtgc
      781 ttgcctccgg ggtaataccc cagatctctg tggtcgtcgg cccgtgcgcg ggcggcgcgg
      841 tctactcgcc ggccataacg gacttcgtct ttatggtcga ggagacgagc cacatgttca
      901 tcaccggtcc cgacgtgata aagagcgtca caggggagga cgttaccttc gaggacctgg
      961 gaggagccgc gacccacaat acccgttctg gcgtggcaca cttctcggcc cccgacgagg
     1021 agatctgctt cgacgacgtg cgctacctgc tctcgttcct gcccgagaac aacctggaga
     1081 gcgctcccta cttccgtcct acggacaacc ccgaacgcaa agacgaggac ctggccgccc
     1141 ttataccgga ctccccccag cgcccctacg atattcgcgg cgccataggc ggcgtcgtcg
     1201 atgacgggga gttcttcgaa gttcaggaag gctgggccca gaacctcgtg gtcggcttcg
     1261 ccaggctgga cgggcgtgcc ataggcgtgg tcggaaacca gccgtccgtg ctcgctggta
     1321 cgctcgacat agacgcgagc gtgaagggtg cgcggttcgt ccgcttctgt gacgccttca
     1381 acattcccct cctcacgttc gtggacgtgc cggggttcat gcctggaacc gaccaggagt
     1441 ggggtgggat catccgccat ggggccaagc ttctttacgc tttctccgag gccacggtgc
     1501 ccaagatgac ggtcataacc aggaaggcct acggtggtgc ctacgacgtc atgtgctcca
     1561 agcacatcgg tgcagacgtc aacgtctcgt ggccgacggg ggagatcgcc gtcatgggag
     1621 cgccggcggc cgtcggcata atcttcaggc gtgagatctc caccgccgaa gaccccgaag
     1681 ccaggcgcac ggagctcatc gagagctacg agcagcagtt caacaaccca atggtcgccg
     1741 ccgagatggg cttcatcgac gacgtcatag acccacgcga gacacgaccg tacctgatca
     1801 aagctctcca gatgatccgt accaagcgcc ccgataggcc accccgtaaa cacggaaata
     1861 tcccactttg agagctacta gctatcagct gtcagctgtc ggccagattc aacccacccc
     1921 gaccccggaa gaggcggctg cgatcgtggc cgcgctggca tcgtttgatg gggagcggga
     1981 agagatgacc tctttgaaac gtagccggtg gcggttggcc gggcttctgg ggcacgaggt
     2041 cgagccgggg atgaagctgg agggctcgct gtgggcctac tccaggtggg agggcatcac
     2101 ctaggtgttc ggcaaggttc ttatcgccaa ccggggtgag atctgcgtgc gcatcgtccg
     2161 cgccctgcgc gagatgggca tctcgagcgt cgccgtctac tcggacgcgg acagggacgc
     2221 cctgcacgcg cgaatcgccg atgaggccta ccacgtcggc ccgacgctgg ccagcgagag
     2281 ctacctgaac gtggggcgcc tgatcgaggt cgccagggag agcggggccg aggcggtcca
     2341 ccccggatac gggttcctgg ccgagagcgc ccccttcgcg cgggcggtgg aggaggccgg
     2401 cctggtctgg atcggccccc accccggggc catcgaggct atggggagca aggtggagtc
     2461 gcgccgaatt atggcgcagg ccaacgtacc tatggttccc ggaacggaag accccatcgc
     2521 ggccgccaaa gaggtacgcc tcttcggcga ggagcatggc ttccccgtgg ctgtgaaggc
     2581 ctcggcgggt gggggcggca agggcttcgc cgtggccgtc gaccattccg aggccgagaa
     2641 cgcgttcgcg cgggcgagca gggagggcga ggcgtacttc ggtgacggct cggtctacgt
     2701 cgagaagtac ctccgggccc cccgccacgt cgagatacag gtcgtgcggg acaaactcgg
     2761 caacgcggcc cacctcgggg agagggactg ctccatccaa cgccgccacc agaagcttct
     2821 cgaggagtgc ccgagccccg ccatagacca gcgaatgagg gaagacatgg gccgggcagc
     2881 cctcgccgcc gccgaggccg tcgactacga caacgtcggc accgtggagt tcctggtcga
     2941 gaacggcgag aacggggacg gcttctactt tctggagatg aacacccgcg tccaggtcga
     3001 gcaccccgta acggaggagg ttacgggcat agacatcgta aagacgggca tccgcatagc
     3061 cgccggtgag ccgcttccct tctcgcaaag agacgttgcc tggcggggtc acgccatcga
     3121 ggtacggctg aacgcggagg accccgcgcg cgacttcgcc ccgagcccgg gcgccgtgac
     3181 cgtctacgag gagcccggcg gtcccggggt gcgggtagac tcctcgctgc gtggccccgg
     3241 gctggtggcc gaatcctacg accccctttt cgccaagctc atcgtcaggg ggggcgacag
     3301 ggaggaggcg ctcgcccggc tggggcgtgc gctcgcggag ttcggggtgg agggcatcta
     3361 cacgacgctc cccttcttcc gtgcgatcct ctctgacgag gccttcgttt cgggcgacta
     3421 ctcgacgggc ttcgtcgccg agaggatggg tctgctggag atagagccaa cgcccccggc
     3481 cgagcctgcc acggatcggg agacctctac tcgcgaggta gaggtcgagg tgaacgggaa
     3541 gctgttcagg gtgaaggtgt tcggggagga gaactcccac ggcccggccg ggcccccgcg
     3601 gcgcaggcag ggtgagacgc gcagggcctc ggcgagtggt ggcgccatcg cggcaccgat
     3661 gcagggaacc atcgtcaggg tgctcgtgga ggtggggcag gaggtggagg cggacgagcc
     3721 cgtgtgcatc ctggaagcca tgaagatgga gagcgaggtc cgctcccaga aggccggcac
     3781 cgtctctgag gtccgcgtcg agtccggcca gaccgtgagg tccggggatc ccctggtcct
     3841 ggtggagtag gtactcacct tggcagaaca cgccgccgag tacgagtggc acggcgaggg
     3901 cgaggaggcg gagatcttcc tctacgcccc cgacgatgcg gtcttcgaac gcgtccttcc
     3961 agccgcccgg ctcccaggtg tccagagccc cgtctacgcg gcggcctcgc cagagggctt
     4021 tggatgggcg accacctctt cctcgcacgt ggcccccgac ttactctccc ttcccgcgcg
     4081 cggcctgctc ctcgtcgcgg gcacgagatc tgagaacctg cgcgaggatg cgcgggagct
     4141 ggcgaacagg atacagcgcg accttccgga ggtaggctca cgcctgccct ccctgaacga
     4201 ggccggggtc ggcaggctct gtgggtcggg cgcgagcgcg gcagcagaag acggcctcat
     4261 cgaagaagac gacctggact tcctggacgc cctgcccggc gacgccgatg ccctgggtcg
     4321 aagggcgctc tccgccggtc cccgcgactg gcaggaggcg gccgtaccag aactacgcgt
     4381 ggtgtccgag gtcttcgaca ccgagggtgc ggatagcctg gggctcgagc ctgggtccct
     4441 ggccatctct atccgggtcg gtgggggaga cctgggctgg ctcgccctct ccgcccacgc
     4501 agagcggctt cgggcgggcc ccgaggactt cggggagggg agggatctcc cctcagcccc
     4561 aaccgagagc ggggaggcgc aagacctcct cgcggcgctg tacgcttcat ccaacttcgc
     4621 cgacgcccgg gccgccagga ccgtgtgggc gttgcgccgc ctgctggacg tcgcaacggg
     4681 ggggctggag atcagggcca cctggaggat gggtggggta gaggagcggg acgggctgct
     4741 ggtacatcgc agggcgctca cccttctcgg ggagggggat gcgctcgtct gcggccgata
     4801 cgctgcattc ggaacgggta acatgtcctc gagcgcgcca cctttcggga tccccgaggc
     4861 agaagacgga tggccgtggg aagaggccgg gctggtggag cgcctggcgg cgttggaccc
     4921 cccggagggc tgaggttgga ccttttgatg gtaagggacc gtaatacggg ccgcttcgtc
     4981 tataccgagc ggctggaacg ccgggccggc gagacctcct gggagtacgt caggcgcagc
     5041 gtcaggaggg agtcccgcat ccgcacccgc ttccacggtg gcaccatgca ggtcatcgtc
     5101 ggctggaacg tagactcggt cgaggagttc ctacgctcgc accccgaata ccgcatcgac
     5161 ggtgtgtccc agtcagaagg acgagacgac agcagcaaag gagacggcgt ttagcggtga
     5221 ggtcctggac aagttctccc aactgataac caccgcgctt ggcctcgtgg ccgcccttgc
     5281 ctggaacacc gccatccaga acctcttcga ccgaatcttt ggcaaagccg gagccgctct
     5341 agccggacag gtcctctacg ccaccatagc cgtaagccgg gccgccgaac gggccaagaa
     5401 acaagaagca gaacaaaaaa ccgaaaccaa cccgggctaa cctccgtcac atagccgggt
     5461 gcaagttggg ttgcacccgg atgcactgta ggagcggttc gcgaaccgcc ccacaaagag
     5521 acgtccaccc aagagagaca tcaagaacaa cgcggttggc gccatgtcac gcgacaccat
     5581 aaaaggcgaa gaagccgccc caggctccaa ctccaaaccc gaagcctgaa gcctaggccc
     5641 ggagggcctt cacttgaaat cttcaggcga gacgcggttc atccaatcct tgaacttgtc
     5701 cacgacctct tcttcgggcg cctcctccat cgcctcctcg aagacgacgc cggcctcctc
     5761 gatcacgtcg tcggaggcga agatctcggc ccccgagcgg atagccaggg cgatggcatc
     5821 ggacggcctc gagtcgacct cgacggtctt gccctctatc tccagcctta tcgtggcgaa
     5881 gaacgtctga tccttcagct cggtaaccgt gatcctctcc atagagccgc cgagctcgtt
     5941 gacgaggttg agggcgaggt cgtgggtcag tggcctgacg aactcctggt tctggagttt
     6001 gaagaggatg gaacgggctt ccgcctggcc gatccagatc ggcaggtaac ggttctcatc
     6061 ttcgaccttg aggatgacga tggggctgga ggaaaacagg tccatgttca tgccgtagat
     6121 ggacatcttg gtgaagccgt cggcgctcac tggctatatg ctcctctcac tccgcgcggg
     6181 gcacacgggg attctattct gtgggccgat tttactgcca gcccccacgg tgttcaatcg
     6241 ggttgtcagt agcctgcccg tgttgtacat tattccggca ccgcgcatct gttgcgcaga
     6301 tcacttaggg cctgtagctc agccggttag agcgcttctc tgataaggaa gaggtccctg
     6361 gttcgagtcc aggcaggccc acccgcagac accttgttct tatgggcaat tcgcaggaat
     6421 cgtggcgaat accgaaacgt tcttctaacc cttttgacac caacgcgagc cgatactatc
     6481 cacggtccct ctcgcagggc aggaagactt ctatcgggcc aggtggcggg gttcttatca
     6541 atctggggca tggatcctgt ccgagcgcct ccggctcacg ggcggccgga gcggcgccag
     6601 ccgagcgggg tgacactgtg gatgcgccga aaggatctca caaagtatcc cgcgtcccca
     6661 tagccgaccc tccgactcac ctcctccacc gccagatccg tctgcaccaa taatctgcgc
     6721 gcttccacca ttcgccgctc gacgatccac tccaggaccg tacgccccgt cttacgcctc
     6781 acgaccgtgg tgaggtagcc cggcgacagg ctcaccgcct ttgctacgtc ctcgagtgag
     6841 atgtgctcgt tgtatctttt ctcgatgaaa ccgaagacct cggcgagtag gggctcctct
     6901 tttagcctga gatcgcccac gacgtccgag gccagccgtc ctatgtctac gagcaaaagc
     6961 gtcaggtgcg ccagtaccgc ctcccggtag ccgtctgtct tgcggcggag ctcgaggttc
     7021 agggcactta agcgctcgga ccacgaggat ctctttgcga cgggcacctt caggttctgg
     7081 acgccttcca tcgcgccccg aacgaagggg aacagaagcg ggtgggcacg ccaggagagg
     7141 aaggcaccgg gcgcttcggg accgaggacc tcgggtggga agaagacggc ccagccttcg
     7201 gctgctacca agccactcgc gtctgcgacc tccccagggg cgatgaggta ggcgtcacct
     7261 gcctccaccc gccactccct ctctcccaaa cgaaatgagc cgcccccgcg ctcgaaatag
     7321 ctgagcagga ggaagtcgtg ggagtgcgca cctggcctta gctccgagaa acctcgatcc
     7381 agcctcatgg cacttaccgg aggcaccccg ggcatcacat cgtaggtgta gaccggcggc
     7441 ccgtcacccc gcttgctcag catccgcgtc gtgcccaagc cgatcgctcc tcttcctgta
     7501 ttggacaacc gcccaaagcc tgcgtagcgc ctgctcgacc gctccgacag acggtgtcct
     7561 cttcgcggat aaggtcgccg caacgctggc ctacactcgg tcatgataaa ccgaagatcg
     7621 tcccataacg gcaaagcttt tgccctttgc aacttccgcc cggctttgaa tactgaacgc
     7681 gtcacattgt ataggcctcg cagcatgagg caggtaagga ggcattcgcc atggacgcaa
     7741 tcggagccag ggatctcgtc gatcggtatt tctccgcgtt gacgaggaga gactggacta
     7801 ctgtgcgcac tctcctgcac gatgacctct tcttcgaggg accgcttgcg acgctcggca
     7861 ctgcggatga gtacctgcaa gggctcgagc atttcacggc gcgcatgacg ggagcggagc
     7921 gccgtatcgt cttcgctgag ggagaagatg tgctccagat ctacgaggtg agcttcgatg
     7981 cgccagcggt cactgcgccg gtcgcggaat ggctccggat ccgcgacgac cgtatcgcaa
     8041 cgatccagat gctcctagac ccgcgcccct tctcgggcgc acagccctcg taatacagca
     8101 caggagcggt gtgtgctcga tggtggcaga ctcctgacgt cctcacggac agccggtcga
     8161 gactgcgagc acgcgggcag gatcgccagc agagtaattc ctcaatatat ggattcaagc
     8221 gctcattagt agctacatct aggaggagaa agttgcacgg tatgcacggc catggagcta
     8281 gccatcaaca tgcacggaac gccccaggct acgccggaac accgccaggg acccggcagt
     8341 ggatcgcatt gatcctgctt tgctttgccc agtttatgct catcgtggac atcacggtcg
     8401 tccaggtcgc tcttccaagc atcggggagg tgctcggcct cggccgcgag tcgctcacct
     8461 gggtggtgac cgcctacacc ctgttcttcg gaggtttgat ggtgttcggt gggcgactgg
     8521 ccgacgcgct cggggcacga cggaccttgc tcgccggtct cgccctcttc acgctcgcct
     8581 ccctgctgtg cggtctcgcg actaccggcg cagcgctcgt cgccggccgc gccgcccagg
     8641 gggtcggtgc cgcgctgctc tctccgtcgg cgctggcgat catcaccacc accttcaccg
     8701 gtgaccaccg tagcaaagca ctgggcatct gggctgcgat tggaggcgtt ggagctgcgc
     8761 tgggtgtgat gctcggcgga atcctcacct ccgggatagg ctggcagtgg gtattcttcg
     8821 tcaacgtccc cgccggactg ctgatcctcc tcatcctccc cactgttgtt ggcagaggcg
     8881 tgtctacctc cgtacgtagg caggtggatg tacccggcgc gctgctggtg accgtcgcga
     8941 ccgcgttgct catctatggc ctcatcaacg ccggcgacgc cgggtgggga gcgggtggga
     9001 ccctcatctc cctgggagcc gccgcggcag cttacttggc attcgtcgtg gtcgaacgtg
     9061 tcgcctctga gcctctgatg cgtgccgaga cgctggcgcg ccgcgcagtg atctccgggg
     9121 cgttcgttat gctggtcgcg acggggctca tgttcgcgct gttcttcctc agctcgtttt
     9181 acctgcaacg gatcctgggg ttcaacgctc ttgaaaccgg actcaccttc cttccggctg
     9241 cgatcgccat aaccatcggc gcccagctcg ctgggcactt tattggcagg ttgggaggcc
     9301 gcaccgtcgc cacggtaggt ttcctgctca ccgccttggg catcggcctt atgacccaga
     9361 tctcaccgca ggagggtgcc ttcacaacgc tcctgcctgg ctttctcatc gccgcgctcg
     9421 gcatcggccc ggccttcgtc gccgccacta ccacggccct cgccgacgtg ccttatgagg
     9481 aggccggtgt cgcctctggg ttagtcaaca ccttccacga gttaggcgga tccatcggcg
     9541 tcgcggccgt ctccacggtc gccgcggcaa gcttcgcccc cagcgccacc ggagtcggcg
     9601 ggttcacaac ggcactagtg gctgctgcgg tcggcgcggg cgctgcggca gtggtcgtgc
     9661 taatcctggt gccccggggc gagccgaccg acaccgtcgt ggggcacggt cacgaaacgg
     9721 gggcacgctg atgatggaga ggacggctat cgtggtggtc ggcggctacg gtgcggtagg
     9781 caggacggtc tgcaccgagt tggctacgcg cttcccgggc cacgtgttcg ccgccggcag
     9841 gaacctggag aaggcgtcgt ccttctcaca agagaccaga ggcagagttt tgcctcttga
     9901 actcgacatc accgatcctt cggccaccgc caaacgtcta gacggcgccg gggtcgtcgt
     9961 ggcgtgcgca gagtcgggcg acgcgacgcg agatgcgggc ctcgcacgtg cgtacctgga
    10021 tcgcggaatc cactacgtgg atgtttcggc ttcttacgcc ctcctcaacg aggtcgagaa
    10081 gctcgacgct ctggccaggc gaagcgccgt cacggcggtg ctgagcgtcg gcctggcgcc
    10141 tggcctcacc aacctgctcg cgaggcagtg cgtcgacgtt ctgggtgacg agctccgcaa
    10201 catagacgtc tccatcctga tcggtctcgg ggaggctcac ggcaaggcgg ccgtccggtg
    10261 gatcgtcgag aacctcgaca agcgcttcga attaccaggc ttcgggacgg tcggcagcct
    10321 cgaggatccg aagaagacgt ttttctccgt cccctacggg gaacgcacct gctaccgttt
    10381 cgacttcgcc gaccagcacg tcttggcgcg cacattgggc gccgagcagg tagccacgag
    10441 gttgtgcttc gactctgccg tggcaacgca ccttttggcg cttgcgaagc aggttggcgt
    10501 cttccgcgcg ctgggaggtc cgcggggaca agacttcctc gtggccctgt tctcgcgctt
    10561 gcgtgttggc tcggacggct tcgaggtcaa ggcagaggcg gaggggaggg aagggggcat
    10621 ctactcttgc tcggctcgcg ggcggggaga agcgcgcacg accgggattg tcgctgcctt
    10681 cgtcgtcgag cggctgctca cttcgccttc gttggagcgt ggtgtgttcc atatcgagca
    10741 gctcttcgag ccggtagagc tgttcggccg gatgaaagag cttgggctca aggtcaacct
    10801 gaaaaacggg cggtccgaat gaatcttcgc tctcggtaga gagtactctt cttaagagac
    10861 cgactcatcc tgcccgttcc tgagaagccc aaataatgca ttggcgaact cacaagcacc
    10921 cgtggggaca gctgcaagac catcaccgtg gcggctagag cggtttgtaa attgagtggt
    10981 ccctgctaga acgaggccca tgaacgcata ctcgaaggat ctcaggttac gcgtgctcgg
    11041 cgccgtggat cgcggtctgc cccgcgagga ggtttcgcgc ctgttcggta tctcccgctc
    11101 gaccatcaag cgctgggtca agcgccgccg cgagggagag gacctcgaac cgaggcgctc
    11161 cacggggcga aagaggcgca tcctctccac cgcg
//
LOCUS       DBNGVD010000348         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_262903, whole genome shotgun sequence.
ACCESSION   DBNGVD010000348 DBNGVD010000000
VERSION     DBNGVD010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_262903"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="AC16CM_09305"
     CDS             complement(<1..333)
                     /locus_tag="AC16CM_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140066.1"
                     /translation="MPDSQHDSTATARDLSGADPTDLDGLQAALAASDPDGELHPIKM
                     HGIEIGEEALGSLIEMVSELARGDLVVLVVDETPMYRGGENLKEMVANTLRESFELEQ
                     VVVGEGEGE"
     gene            complement(421..1926)
                     /locus_tag="AC16CM_09310"
     CDS             complement(421..1926)
                     /locus_tag="AC16CM_09310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3140067.1"
                     /translation="MGESKSELLLGVDIGTSGTSGVIARPDGEVVVTAEKDHNVSLPR
                     PGWVEHDAEEDWWNGFADVCSELLEQADGEVVAVSISGIGPCLLAADGNGRPLRPGIL
                     YGVDTRATSEVEELTERYGTEKIMEVGGNPLTSQSVGPKILWLQRNEPEVWRETRQIL
                     MAHTYIIHRLTGEYVLDHPSASMCDPIYDVENYRWVEGWAEEIAPGLELPDLRWPSEV
                     AGVMTRDAAEATGLPAGIPVITGSTDAFSEAVSVGVRDPGDLMLMYGTTMATLEISEE
                     PLATRSLWSTAGLFEDTYGLFAAMSTSGALTNWVRKICGGKSFGELTEDARQVAPGSD
                     GLLVLPYFAGERTPVSDPKARGVICGLTLSHGQGHLYRAMLEATAFGVRHLFEAMDEA
                     GGKKKRLVAVGGGTKGGLWTQIVSNTIGEPQELPEQTIGASYGDALLAGIATDLVGAD
                     ADWSRIVETVEPDPSEQEIYDELYTVYRELYPATRDQAHTLTDIQIRQGEA"
     gene            complement(1992..2906)
                     /locus_tag="AC16CM_09315"
     CDS             complement(1992..2906)
                     /locus_tag="AC16CM_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140068.1"
                     /translation="MAEIYDHLGRPVEHGRIRVNGIRMHYVTAGEGEPLLLLHGTPKT
                     HYYWYKLIPLLTESFTVVAPDLRGFGDTDRPTAEEGYDSRTNADDLAELMTSLGHDRF
                     HVHGEDRGAEFAYVLTATRPERVMTLSFAEMLLSGEGLEEWSYFTPENVSAQFDLRGV
                     WLWHIPFFWIPDIPEMLITGKEREFWEFWIKAETYNPTAIADEAIDEWISHLKAPGGL
                     RGVLETYRAGFKNASINGELKQTRISAPVLTIGAPEFFGNLVEDQMRKVAESVERSEV
                     FEECGHSIALEAEDRLADLLREFMLDRQ"
BASE COUNT          538 a         1132 c          985 g          650 t
ORIGIN      
        1 ttccccttcg ccctcgccta ccaccacctg ctccagctcg aagctctccc tcagggtgtt
       61 ggcgaccatc tccttgaggt tctctccgcc acggtacatc ggcgtctcgt ccacgaccag
      121 caccaccaga tcgccacggg ccagttcgga gaccatctct atgagggagc ctagcgcctc
      181 ttcgcctatc tcgatcccgt gcatctttat cgggtggagc tccccgtccg ggtcggaggc
      241 cgctagggcc gcctgcaagc cgtcgaggtc ggtgggatcc gccccggaga gatccctggc
      301 tgtggccgtg ctgtcgtgtt ggctgtcagg catgtgccct actttctatt cgaggtactg
      361 cgagccattc gcgaggtatc tgctgaaggt tgttgcgggg cattacctcg ctggtctcgt
      421 ttacgcttcg ccctgtctga tctgtatatc ggtcagggtg tgagcctgat ctcgcgtcgc
      481 ggggtagagc tctctgtaga cggtgtagag ctcgtcgtag atctcctgct cggatgggtc
      541 gggctcgacg gtctccacga tacggctcca gtcggcgtcc gccccgacga ggtcggtggc
      601 gatgcctgcc agcagggcgt cgccgtagct ggcacctatg gtctgctctg gtagttcctg
      661 gggctcaccg atggtgttgg agacgatctg ggtccataat ccgcccttcg tgccgccccc
      721 gacggcgacg aggcgcttct tcttaccgcc agcctcgtcc atggcctcga agaggtgacg
      781 caccccgaag gcggtagcct cgagcatggc ccggtagagg tgcccctgcc cgtggctcaa
      841 ggtaaggcca cagatgacgc cacgcgcttt cgggtcgctt acgggggttc gctccccggc
      901 gaagtacggc agtaccagga gcccatccga gcccggggcg acctgtcggg catcctccgt
      961 aagctccccg aacgacttgc ccccgcagat cttcctcacc cagttggtca gtgcccccga
     1021 ggtagacatc gcggcgaaga gcccgtaagt atcctcgaag agccccgccg tgctccacag
     1081 gctgcgggta gccaacggct cctcggagat ctccagcgtc gccatggtgg tgccgtacat
     1141 gagcataagg tcgcccgggt cccgcacccc gacgctaacc gcctccgaga aggcgtccgt
     1201 gctaccggtg atcacgggga taccggcggg gaggcccgtc gcctccgccg catccctggt
     1261 catcacgccc gcgacctcgg agggccagcg tagatcgggc aactcgagac cgggggctat
     1321 ctcttcggcc cagccttcca cccaccggta gttctcgacg tcgtagatgg gatcgcacat
     1381 gctcgccgac gggtggtcca gcacgtactc gccggtcagg cggtggataa tgtaggtgtg
     1441 ggccatcagg atctggcggg tctcccgcca tacctcgggc tcgttgcgct ggagccagag
     1501 gatcttgggg ccaaccgact gggacgtcag agggttgccg cccacctcca tgatcttctc
     1561 ggtcccgtat ctctccgtaa gctcctcgac ctcactggtg gcccgggtgt cgacgccgta
     1621 aaggatccct ggcctcaagg gcctgccgtt gccgtcggcc gcgagcagac aggggccgat
     1681 gccgctgatg ctcacggcga ccacctcacc gtcggcctgc tccagtagct cggagcagac
     1741 gtccgcgaag ccgttccacc agtcctcttc ggcgtcatgc tcgacccacc ccggcctcgg
     1801 aaggctgacg ttgtggtcct tctcggcggt tacgacgact tcaccgtcgg gcctcgcgat
     1861 aacgcccgag gtgcccgatg tgcctatgtc cacccctagc aggagctcgc tcttgctctc
     1921 tcccacgtca gatctccctt ccacgtgacg tcacgagaac tccgacaccg acgccaccgt
     1981 cccgccctcg actattgcct gtccaacatg aactcgcgca acaggtcggc gagcctgtct
     2041 tcggcctcga gcgcgatgct gtggccgcac tcctcgaata cctctgaccg ctcgacagat
     2101 tcggcgacct tgcgcatctg gtcttcgacg aggttcccga agaactccgg cgccccgatc
     2161 gtcaggaccg gcgccgagat cctggtctgc ttgagctcgc cgttaatgct ggcgttcttg
     2221 aacccggccc ggtacgtctc gagaacgcca cgaagcccgc ctggcgcctt caggtgggag
     2281 atccactcgt ctatcgcctc gtccgcgatg gccgtggggt tgtacgtctc ggccttgatc
     2341 cagaactccc agaactcgcg ctctttcccg gtgatgagca tctccggaat gtccgggatc
     2401 cagaagaacg ggatgtgcca gagccacact cccctgagat cgaactgcgc agatacgttc
     2461 tcgggtgtga agtaggacca ctcctcgagc ccctctccgg aaagaagcat ctcggcgaat
     2521 gagagcgtca tcaccctctc gggcctggtg gcggtgagga cgtaggcgaa ctcggccccg
     2581 cgatcctcgc cgtgcacgtg gaacctgtcg tggccgaggc tggtcatgag ctccgcaagg
     2641 tcgtcggcgt ttgtacgcga gtcgtaccct tcctcggcag tgggcctgtc cgtatcgccg
     2701 aaaccccgga ggtccggcgc aaccaccgtg aagctctccg tcagcagagg gatcaacttg
     2761 taccagtaat agtgggtctt gggtgtaccg tgcaggagca ggagcggctc gccctcaccg
     2821 gccgtcacat aatgcatcct gatgccgttc accctgatcc gaccgtgctc tacaggtcta
     2881 cccagatgat cgtagatctc agccatcgac ctcccctatc ctggctcccg gcgagtctac
     2941 acgatccgcc gctcccacaa ccccaccccc gccaacgacg gggaccagag gccccagaac
     3001 gctccgcata tcctcacgaa gccgtcgttg ctcgtctctc gccgccaccg aactacctca
     3061 cgctgtggcg caaatgttca gcacgagggt acttatatca atcatcttcg gtgcttacaa
     3121 taccctgacg cgccatcggg acgacgcccg gctacttcgc gggcgtcgtg tgctggagac
     3181 cggaatgtag cgggagaggc ttcgggttat acccctgccc ggacggacac cgggtctcgc
     3241 gtcgctgccg cgagacagag ctctcttgcg gtgtcgaggg cctctgggac agacgaaaag
     3301 tggtc
//
LOCUS       DBNGVD010000349        10047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_626442, whole genome shotgun sequence.
ACCESSION   DBNGVD010000349 DBNGVD010000000
VERSION     DBNGVD010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_626442"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="AC16CM_09320"
     CDS             complement(<1..2131)
                     /locus_tag="AC16CM_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3140069.1"
                     /translation="MICENCGIRPASLQVFQQQGDRRVTSYFCSQCARQLGMIGGAQG
                     GADPFAKLQNLIQQQQGGQGSLFDALSAEAQEAVMRAREASAGTTRAAAIGTDHLLAG
                     IVTGENVATEALERAGADVSGMRARFQEFRGDPGEEVYTFTPSSKRALQLSFAAARQM
                     GAGQVGSEHLLLGLLGEGDGNAYRILQQHGANDAEALRREILRLMQQRGAQMGPGAGM
                     PGAAAGGPGMGGGQQGTGSNTPTLDQVSRDLTQSARDGELDPVIGRAEEISRVVRILS
                     RRTKNNPVLIGEAGVGKTAVAEGLAQRIVSEDIPESLKGKRVLALDVGGLVAGTRFRG
                     DFEERMQQMLKELQQEQRDIILFIDELHTIVGAGGADGAVNASNMLKPALARGELQVV
                     GATTLDEYRKHVEKDPALERRFQPVLVDEPTVDETVAILFGLRDRYEAHHRVRITEEA
                     IIAAAQLGDRYITDRFLPDKSIDLLDEAAAEVRLRSTVPPVDVKRIEEEIAGLEREKE
                     DAVRAEDYEKAAGFKQRVEQLKAELDEEQEGWAGRRESNAPEVTREDIARILEEWTGV
                     PATNIVQEEAERLLNLEGVLHERVVGQEPAVRAVSEAIRRARAGIKDPRRPVGSFIFL
                     GPTGVGKTELARTLAQYLFGEEDAMIRIDMSEFQEAHTVSRLVGAPPGYVGYEEAGQL
                     TEQVRRRPYSVVLFDEIEKAHPDVFNTL"
     gene            complement(2317..3753)
                     /locus_tag="AC16CM_09325"
     CDS             complement(2317..3753)
                     /locus_tag="AC16CM_09325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3140070.1"
                     /translation="MPVRWRLTIFNALAIGAILVALGLVLFFVLRATLLSSVEETAQS
                     RAFAAARSIADGEGLEEEEGRLVLDDDLVDGITVDGAYIIVRDGRGNVIAQTLDLPAA
                     REARDEVWRVALEAGRPAEGVLELSGEGTDYVYAVPVRPYGGPARTVEAGKSYALAAE
                     NVREVALVLAGGIGAAFLLSVVGAYLLARAALSPVAAIARAARKITGGDLSQRLPVAR
                     PKDEIGDLAATFNGMLSRLEGTLADLKKALARQRRFAADASHELRTPLTSIHSNAQLL
                     EEWGLKDERIGPESVAAIRRESERMRRLIEGLLELTRGDEGATLRLEHQDLTVVADEA
                     TEAARTVANGRVTIEHPPSREPVDAVFDRERLRQAISILLDNAVKYTPEGGRISLEVS
                     EEPDAVSVIVSDTGIGIPEDRLPHVFERFYRAEEARSTQGSGLGLAIARQIVEDHGGS
                     LRARSRPGEGSTFTIRIPRHIPPDGTDT"
     gene            complement(3754..4431)
                     /locus_tag="AC16CM_09330"
     CDS             complement(3754..4431)
                     /locus_tag="AC16CM_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3140071.1"
                     /translation="MRPGTRVLIVEDDHSIARVVRLQLEHRGFAVLCRHDGISGLEAV
                     SEFRPDVIVLDIMLPGMDGVGVLQHLRTEGSRVPVIMLTARDTPLDKIHSLDHGADDY
                     LTKPFDMGELMARIRAVLRRVEGEEVLRIEDLEIDTAAHAVRRGERGVDLTAREYELL
                     VFMARNARRVLSRETLLSRVWEQDFDVGTNVVEVYVSYLRRKVDGPGEKRLIHTVRGA
                     GYVLRGG"
     gene            4619..5167
                     /locus_tag="AC16CM_09335"
     CDS             4619..5167
                     /locus_tag="AC16CM_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140072.1"
                     /translation="MKRLTILVLLVTTLALGACSAQTREPQADGSSGASERPKTVVVY
                     KDAGEADDGGSDAGKPREGGGILVNDDDPDGVDDLDADERMRSGQRLPADFPIPVPDE
                     YRVGAVGVAGNETAVILSVPSGEDAYNYYRQFLTDRGFRLVDEGRSQGGFLDAELEFS
                     NNALEGSMDFDGDTVEVDIERF"
     gene            5312..5692
                     /locus_tag="AC16CM_09340"
     CDS             5312..5692
                     /locus_tag="AC16CM_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140073.1"
                     /translation="MRKLVIMVTMVSVLGALFAGAALAANLVGNDRDNRLVGTNSADT
                     IRGKGGEDDIYGLRGQDRLYGGTGEDDIEAGRGNDFVGAVDRSEDDVECGPGDDRVRA
                     NPGDDVEGCERVVRDGPRVGDRDD"
     gene            5933..6607
                     /locus_tag="AC16CM_09345"
     CDS             5933..6607
                     /locus_tag="AC16CM_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR3140074.1"
                     /translation="MQERSLIGLEARAADGTEVGRIVEVVTDEEDGGVTFVIVETEDG
                     EQQEVPITSLSLDPEVDFATFRADASDVEPGDHTGDEVGVPAPAPEEVDDPEEEDYEH
                     EGQLVTESEDENEAGLVEDLRTREDWEDESYTSDSGYPRNDVYIDPDTGATDVDPLLS
                     DVDSLRHDVAGLLDGTNLGVGNVREGVVELTGASRTREDLAEVVREVRGLDGVLEIDT
                     TDVDVG"
     gene            complement(6790..9861)
                     /locus_tag="AC16CM_09350"
     CDS             complement(6790..9861)
                     /locus_tag="AC16CM_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR3140075.1"
                     /translation="MSRIEELSGRCLHELFEEQARRTPEAVALEDSEVSLTYAELDSL
                     SGRLASYIRSKGIGPDDPVGVFMERRAEYVIACLAAMKAGGAYLVLELAYPPPLLADV
                     MSDAGPRLVLTQERYTDRLPEGTETFCLDAALDERLEDLPAVDERVEVGRDNLAFISY
                     SAGTTGKPKGIANPHKASVLSYLWRFGISDYEPGDRVGCNVFFIWEMIRPLLRGATTV
                     VIPDDVIYDPNALVRFIEEYGITEVLITPSLLETVLNSGGSDLEERLSSLKTLWLNGE
                     VVTRTLAQRAMGVLPHTRLLNVYSCSETHEVAAGDLSELVENQESTYCPVGVPMDRDR
                     LYLLDEDGEHVPEGEPGELFVGGECLAREYVGLPEKTEESFPEDRFSRAEGARMYRSG
                     DRARILSDGTLEIMGRVDFMVKIRGYSVELGAVEAAIEEDLAVQACVVVSEGEEGEDK
                     RLVAYLVPDPDDEDRYSGWSLDPKTGRSKEMRGVLQDSLPHYAVPSVFVELESLPIQA
                     TSGKVDRTELPPPPPRPTRRETGDIERLPDDASHREKEALLARAWENVLRLDEGEVGP
                     EDNFFDLGGHSLAAAQLSSQVEQGFGVHVSMPLFMEDPTLVGLLGKIEQLQLDGTAGT
                     VTAVGDLISDAALDPEITPQPADETPTLDDARDVFLTGTTGFLGAFLLDGLLSSTEAR
                     VHCLVRPRGDAMGTIEANLRRYGLWKPGHAERIVPVPGDLAAPLLGLDEEGFDALARR
                     VDLIVHAGAAVNLVYPYSELKAPNVGGTNEILRLACRNGARPVHYVSTNGIFPSDSGP
                     CEEDVDLDRLADAREDGYGQSKWVAEKLVREAGKRGLPVSVYRPGNVSGHSETGASNP
                     RDLLGAVIVESLRLGCAPEVEDWRMEMTPVDFVAEAMVHSASNPGSYGGTYHLANPEP
                     PHADDVFDLLEERGYTLERVPYEEWLQKIDADPPEEGTPAEIVNGAAPAADELGDDND
                     YDDTNTRRVLGDDGPARPPVDEDLLETYVRYFAQQGWIEAPAALQEVQKGR"
BASE COUNT         1649 a         3393 c         3286 g         1719 t
ORIGIN      
        1 gcagcgtgtt gaacacgtcc gggtgggcct tctcgatctc gtcgaagaga acgacgctgt
       61 agggacgacg cctgacctgc tccgtgagct gtccggcctc ctcgtagccg acgtagccgg
      121 gaggggcacc gacgagccgg gagacggtgt gggcctcctg gaactcggac atgtctatcc
      181 gaatcatggc gtcttcctca ccgaagaggt actgggcgag cgtccgcgca agctcggtct
      241 tacccacgcc cgttggcccg aggaagatga agcttccaac cggccgcctg gggtccttta
      301 tgccggccct ggcgcgcctg atggcctcgg agaccgccct gaccgcgggc tcctggccca
      361 cgacccgctc gtgcagcacg ccctccaggt tgagtagccg ctcggcctcc tcctggacga
      421 tgttggtggc tggcacgccg gtccactcct ccaggatgcg ggcgatgtcc tcccgcgtca
      481 cctcgggtgc gttggactcg cgacgtccgg cccagccctc ctgctcctca tcgagctcgg
      541 ccttgagctg ctcgacgcgt tgcttgaaac cggcggcctt ctcgtagtcc tcggcccgca
      601 cggcgtcttc cttctcacgc tcgaggcccg ctatctcctc ctcgatgcgc ttcacgtcga
      661 ccggcggcac cgtggaacgg agcctcacct cggcagcggc ctcgtcgagc aggtctatgg
      721 acttgtcagg caggaaacgg tccgtgatgt agcggtcgcc gagctgggcc gccgcgatga
      781 tggcctcctc ggtgatcctg accctgtggt gcgcctcgta acggtccctg aggccgaaga
      841 gtatggccac ggtctcgtcc accgttggct cgtccacgag cacgggctgg aagcgccgct
      901 ccagcgccgg gtccttctcg acgtgcttgc ggtactcgtc gagcgtggtt gccccgacga
      961 cctgcagctc gccgcgagcg agcgccggct ttagcatgtt cgaggcgttt accgcgccgt
     1021 cggcgccgcc ggcccccacg atggtgtgca gctcgtcgat gaagaggatg atgtccctct
     1081 gctcctgctg cagctctttg agcatctgct gcatccgctc ctcgaagtcc ccgcggaaac
     1141 gggtgccggc cacgagcccg ccgacgtcta gcgccagcac acgcttgccc ttgagcgact
     1201 ccgggatgtc ctcggagacg atgcgctggg cgagcccctc ggcaacggcc gtcttgccca
     1261 cgccggcctc gccgatcagg acaggattgt tcttggtgcg cctggagagg atacggacta
     1321 cccgcgagat ctcctcggcg cgtccgatca ccgggtcgag ctcaccgtcc cgcgcggact
     1381 gcgtgaggtc cctgcttacc tggtccaggg tcggcgtgtt gctgccggtt ccctgctgac
     1441 caccgcccat gccgggccct ccggcggcgg cgccgggcat gccggcacct gggcccatct
     1501 gcgcgccgcg ctgctgcatg aggcgcagga tctcacgcct caaagcctcg gcgtcgttgg
     1561 ccccgtgctg ctgcaatatt ctgtacgcgt tgccgtcgcc ctcgcctagc agcccgagca
     1621 acaggtgctc gctcccgacc tgcccggcac ccatctgcct ggcggcggcg aacgagagct
     1681 gcagggcccg cttggagctg ggggtgaacg tgtagacctc ctcgcccggg tcgccgcgga
     1741 actcctggaa ccgcgcccgc atgccgctca cgtcggcacc ggcacgctcc agcgcctctg
     1801 tcgccacgtt ctcaccggtc actatgcccg cgagcaggtg gtcggtcccg atggccgcgg
     1861 cccgcgtcgt gcctgccgaa gcctcgcggg cacgcatcac tgcctcctgc gcctccgcag
     1921 agagcgcgtc gaacaggctg ccctgcccac cctgctgctg ctgtatcagg ttctggagct
     1981 tcgcgaacgg gtcggcccca ccctgcgcac ccccgatcat gccgagctgc cgcgcgcact
     2041 gcgagcagaa gtaactcgtc acccgccggt ccccctgctg ctggaaaacc tgcaaactgg
     2101 ccggcctgat cccacaattc tcacatatca tccgtctaaa gcctcctggt tgaaatcttc
     2161 tcgttccctg gtaccccatg ccgagacaaa cgacctatcc aaaatctaag cctggcacgc
     2221 tcatatttta ttaccacccc acgggagatc aaggttttga cggcgatctt gccgtccggg
     2281 actcgctggt acgatgggga ggccgggctg gggcggtcag gtatccgttc cgtctggcgg
     2341 gatgtgtctg gggatacgga tcgtgaaggt cgagccttcg ccgggtctgc tccgcgcccg
     2401 caagctaccg ccgtggtcct cgacgatctg tcgcgctatc gccagcccca acccggagcc
     2461 ctgcgtcgag cgggcctctt cggcgcggta gaaccgctcg aagacgtggg ggagccggtc
     2521 ttcgggtatg ccgatcccgg tgtccgaaac gattacggag acggcgtcgg gctcttctga
     2581 cacctccagg gagattcgtc ccccttctgg ggtgtacttt acggcgttat cgaggagtat
     2641 cgagatcgcc tgcctgagcc tttccctgtc gaagactgcg tccaccggct ccctggacgg
     2701 ggggtgttcg atggtcaccc ggccgttcgc gaccgtgcgc gcagcctccg tcgcctcgtc
     2761 ggcgaccacc gtaagatcct ggtgctcaag gcgtagcgtc gccccctcgt caccacgggt
     2821 cagctccagg aggccctcga tcagcctgcg catgcgctca gattcgcgcc tgatcgccgc
     2881 cacgctctcg gggccgatcc gctcgtcttt caggccccat tcctcgagca gttgcgcgtt
     2941 gctgtgtatg gaggtcaggg gggtacgaag ctcgtggctg gcgtcggcag cgaagcggcg
     3001 ctggcgggcg agcgccttct tgaggtcggc cagggtacct tccaggcggg agagcatgcc
     3061 gttgaaggtg gccgcgaggt ccccgatctc gtctttcggc ctggcgaccg gtagcctctg
     3121 tgacaggtct ccgccggtga tcttacgtgc ggcccgcgct atcgcggcca cgggggagag
     3181 ggcggcgcgg gcgagcaggt aggccccgac caccgagagg aggaacgccg ccccgatgcc
     3241 gccagccagc accagggcca cctcgcgcac gttctcggcg gcgagtgcat aggacttgcc
     3301 cgcctccacg gtacgcgctg ggcccccgta cggccgtacg gggacggcgt agacgtagtc
     3361 ggtaccctcg cccgaaagct cgagcacgcc ctccgcgggc ctgccggcct ccagcgctac
     3421 gcgccacact tcgtcgcgtg cctcgcgggc ggcgggcagg tcaagggtct gggctatcac
     3481 gttgcccctg ccgtcccgca cgatgatgta cgcgccgtcg acggtgatgc cgtccacgag
     3541 atcgtcgtcg aggacgagcc ggccttcctc ctcctccaga ccttcgccgt cggctatgga
     3601 ccgggctgcg gcgaaggccc ggctctgggc cgtctcctcg acgctggaga gcagcgtcgc
     3661 ccgtagcacg aagaacagga cgaggccaag cgccaccagt atggccccga tggcgagcgc
     3721 gttgaagatg gtgagcctcc agcgcaccgg cacctaacca ccccgcagta cgtacccggc
     3781 cccacgcacg gtgtggatga gccgcttctc gccgggaccg tccacctttc tccggaggta
     3841 gctgacgtac acctccacga cgttggtccc gacgtcgaag tcctgctccc aaacccgcga
     3901 caggagcgtc tcccgcgaga gcacccgcct ggcgttgcgt gccatgaaca ccagcagctc
     3961 gtactcccgg gcagtaaggt cgaccccgcg ctccccgcgc cgtacggcgt gggcggctgt
     4021 gtctatctcc aggtcttcga tgcggagcac ctcctcaccc tcgaccctgc ggagcaccgc
     4081 gcggatgcgg gccataagct cgcccatatc aaagggcttg gtgaggtaat cgtcggcgcc
     4141 gtggtcgagg ctgtggatct tgtccagcgg cgtgtccctg gcggtcagca tgatgacggg
     4201 aaccctgctc ccctccgtcc tcaggtgctg gaggaccccg accccgtcca tccccggcag
     4261 catgatgtcg agcacgatca cgtccggcct gaactccgag accgcctcca gccccgagat
     4321 cccgtcgtgg cggcagagca cggcgaagcc gcgatgctcg agctgcagcc gcacgacgcg
     4381 cgctatggag tgatcatcct cgacgatcaa gactcgggtt ccgggccgca tgactcctcc
     4441 atcgggctgc ttgccgcgtg gcttcgaccg ttgatcataa ccgccctacc ggggaaaggg
     4501 acgtgagatc cggggccagc tcgcggattc taagcaaact ctaatgctgc tttcagcttc
     4561 tccttaccac gtagcggaac aatcagacga aagagaccca ggcaagagag gagacggtat
     4621 gaaaaggctc accatccttg tgctactggt gacgaccctg gcgttggggg cctgctcggc
     4681 ccagacccgg gagccacagg ccgacggctc gtccggtgct tccgagcggc cgaagacggt
     4741 cgtggtctac aaagacgcgg gtgaggctga cgacggcggc agtgacgctg gcaaaccgcg
     4801 ggaaggcggg ggtattctcg tgaacgacga tgacccggat ggcgtggatg acctcgacgc
     4861 cgacgaaagg atgcgaagcg gccagcggct gcccgccgac ttccccatcc cggtgccgga
     4921 cgagtatcgg gtcggggccg tcggcgtggc cggcaacgag acggcggtca tcctcagcgt
     4981 accctcgggc gaggacgcct acaactacta caggcaattt ctcaccgaca gaggcttccg
     5041 cctcgtggac gagggacgca gccagggcgg gttcctggac gccgaactgg agttctcgaa
     5101 caacgcgctc gagggcagca tggacttcga cggcgatacc gtcgaggtgg atatagaacg
     5161 gttctagggg ccctcaggac attctaatag acccctaacg ggcctctaac gcagctctaa
     5221 ttatcgcccg ataggatgcg acaacgatcc atcaacggta ccggcagggg cgcagagcgc
     5281 cgacgggacc acaaccaaga ggaggttgga gatgaggaaa ctggtcatca tggtgacgat
     5341 ggtgagcgtg ttgggagccc ttttcgccgg cgccgcgctg gcggcgaacc tggtcggtaa
     5401 cgaccgcgat aatcgccttg tcgggaccaa ctcagccgac accattagag gcaagggcgg
     5461 tgaggacgac atctacgggc ttcgcggcca ggacaggctc tacggcggca ccggcgagga
     5521 cgacatcgag gccggcaggg gcaacgactt cgtcggcgcg gttgaccgct cggaggacga
     5581 cgtggagtgc ggacccggcg acgacagggt cagggccaac cccggcgatg acgtcgaggg
     5641 ctgcgagagg gtcgtgaggg acggacccag ggtgggggac cgggacgact gagctctgta
     5701 accctcctgg ccgctatctg cagcggggcc gtggcatcgt gccgcggtcc cgcgtttacc
     5761 cggggttttg ttcggtgcta ttttcaggcg caatgttgcc gtagggcggt tcgtggttgg
     5821 gcggttcgcg aaccgcccct acagcgagga tgcggggcgc cggcagggtt gccgaatgct
     5881 gcttacgggc acggggtata tttggaccga gaaggctaat ctggaggatg acttgcagga
     5941 gagaagtcta atcgggctcg aggcccgggc ggctgacggc acggaggtcg gacgcatcgt
     6001 ggaggtggtg acggacgagg aggatggggg ggtcacgttc gtcatcgtgg agacggaaga
     6061 tggggagcaa caggaggtgc ctatcaccag cctctccctc gatcccgagg tggatttcgc
     6121 caccttccgg gccgacgctt cggacgtgga gcctggggac cacacgggcg atgaagtagg
     6181 ggtcccggcc cctgcccctg aggaggtcga cgacccggaa gaagaagact acgagcacga
     6241 gggccagctc gtcaccgagt ccgaagacga gaacgaggca gggctcgtgg aagacctcag
     6301 gacgcgggag gactgggagg acgaatcata cacctcggac tccggatatc cccgcaacga
     6361 cgtctacatc gaccccgaca ccggcgcgac ggacgtggac ccactcctca gcgacgtcga
     6421 cagcctcagg cacgacgtgg ccggcctgct cgacggcacg aacctggggg ttggcaacgt
     6481 tagagagggc gtcgtcgaac ttaccggcgc gtcccgcacg cgggaggacc tcgcggaggt
     6541 ggtgcgggag gtcagaggtc tcgacggggt gctcgagatc gacaccacgg acgtggacgt
     6601 gggttagggg gttgcccggg cggtgcagct gaccctgggc cgccatcgcc aggagggcgg
     6661 cgaccgcagc gccccaggac aagcgaccat gagacgggaa catctgcgta tcctacgatc
     6721 ggccggtccg tgcagggcac ccacgcggtc cgccgtagcg ggcggtatcg gcccttcgca
     6781 agaagtaacc tacctgccct tctggacctc ctgcagagcc gcgggcgctt ctatccatcc
     6841 ctgctgggca aagtaccgta cgtaggtctc cagcaggtcc tcgtcgacgg gtggcctcgc
     6901 cggtccatcg tcgccgagga cgcggcgcgt gttggtgtcg tcgtaatcgt tgtcgtcccc
     6961 gagttcgtct gccgcgggcg ccgccccgtt tactatctcc gcgggggtac cttcttcggg
     7021 ggggtcggca tctatcttct gcagccactc ctcgtatggg acccgctcca gcgtgtagcc
     7081 tcgctcctcg agcagatcga aaacgtcgtc ggcgtgcggg ggttcggggt tggccagatg
     7141 gtaggttccc ccgtaagaac cagggttcga ggcactgtga accatcgcct cggccacgaa
     7201 atcgacgggg gtcatctcca tgcgccagtc ttccacctcc ggtgcgcagc ccagccgcaa
     7261 cgactccacg atcacggcgc cgagcaggtc gcgcgggttg gaggctcccg tctcgctgtg
     7321 ccctgaaacg ttgccgggac ggtacacgct gaccggcaga ccccgcttgc cggcctcccg
     7381 caccagtttc tcggccaccc acttggactg accgtagccg tcctcgcggg cgtcggcgag
     7441 gcggtcgagg tccacatcct cctcgcaagg gcccgagtcc gacgggaaga tcccgttggt
     7501 cgacacgtaa tgaacaggcc tggccccgtt ccggcacgcc aggcgcagga tctcgttcgt
     7561 gcccccaacg ttgggtgcct tcagctccga gtaaggatac acgaggttca ctgccgcccc
     7621 ggcgtggacg atcaggtcca ccctgcgggc cagcgcgtcg aagccttcct cgtccagacc
     7681 cagcagtggt gcggccagat cccccggaac gggcacgatg cgctcggcgt gcccgggctt
     7741 ccacagcccg tagcgtcgca ggttggcctc gatggtcccc atcgcgtcac cgcggggcct
     7801 gaccaggcaa tggacgcgcg cttcggtgct ggagagcaac ccgtcgagca ggaaggctcc
     7861 caggaacccg gtcgttccgg tcaggaaaac gtcgcgggca tcgtccaggg tcggggtctc
     7921 gtcggcgggt tgtggggtta tctcggggtc gagtgcggcg tcggagatca gatctccaac
     7981 ggccgtcacc gttcccgccg tcccgtcgag ctgcaactgc tctatcttgc cgagcagccc
     8041 aaccagcgtc gggtcttcca tgaacagggg catggagacg tggacgccga agccctgctc
     8101 gacctggctg gagagctggg cggcggccag cgagtgcccg ccgaggtcga agaagttgtc
     8161 ctcgggtccc acctcaccct cgtcgagacg cagcacgttc tcccaggccc gcgcgagcag
     8221 cgcctccttc tcccggtgag aggcatcgtc gggaagcctc tctatatcgc ccgtctccct
     8281 gcgagtgggc cgcggcggag gcggcggaag ctcggtgcgg tcgaccttgc ccgaggtggc
     8341 ctggataggt agcgactcga gctcgacgaa gacggagggg acggcatagt gcggtaggct
     8401 gtcctgcaga acgcccctca tctccttgct gcggcccgtc ttcggatcca ggctccagcc
     8461 ggagtagcgg tcttcgtcat cagggtcggg caccaggtag gccacgaggc gcttgtcctc
     8521 gccctcctcg ccctccgaaa cgaccacgca agcctgcacg gccaggtctt cctcgatggc
     8581 ggcctccacg gcgccaagct ccacgctgta gcccctgatc ttgaccataa agtcgacgcg
     8641 gcccatgatc tccagggtcc cgtcggagag gatgcgcgcc ctgtcaccag accggtacat
     8701 cctggcaccc tcggcccggg agaagcggtc ttcgggaaag ctctcctcgg tcttctcggg
     8761 caggccaacg tactcacgcg cgaggcactc gccgccgacg aaaagctcgc cgggctcgcc
     8821 ctcgggaacg tgttcgccgt cctcgtccag gaggtagagg cggtcccggt ccattggaac
     8881 gccgaccggg cagtacgtgg actcctggtt ctctaccagc tcgctcaggt ccccggcggc
     8941 aacctcgtgg gtctcgctgc agctgtagac gttcaggagg cgggtgtggg gcaagacccc
     9001 catggcgcgc tgggcgagcg tgcgggtgac cacctcaccg tttagccaca gcgtcttcag
     9061 gctcgacagc ctctcttcca gatccgaccc acccgagttc agaaccgtct ccaggaggga
     9121 aggcgtgatc aggacctccg tgatcccgta ctcctcgatg aaccggacca gggcgttggg
     9181 atcgtagatt acgtcgtcgg ggatgacgac cgtggtcgcc ccccgcaaga gcggccgtat
     9241 catctcccag atgaagaaca cgttgcaccc gaccctgtcg cccggctcat aatcgctgat
     9301 gccgaaccgc cacaggtagg agaggacgga agccttgtgc gggttggcta tcccctttgg
     9361 cttccccgtc gttccggccg agtaggagat gaaggccagg ttgtcccgcc cgacctccac
     9421 cctctcgtcc acggccggaa ggtcctcgag cctctcgtcc agagccgcgt cgaggcagaa
     9481 cgtctccgta ccctccggca ggcgatccgt gtaccgctcc tgcgtgagca cgaggcgcgg
     9541 accagcgtcg gacatgacgt cggcgagcag gggcggtggg taggcgagct cgagcaccag
     9601 gtatgcgccg ccagccttca tggcggcgag gcacgcgatc acgtactcgg cccgccgctc
     9661 catgaagaca ccaacgggat cgtccggccc tatgcccttg ctcctgatat aagacgccag
     9721 cctcccggag aggctatcca gctcagcgta ggtcagggac acctcactgt cctcaagcgc
     9781 gacggcctcc ggcgtgcgac gggcctgctc ctcgaagagc tcgtgcaggc agcgaccgga
     9841 aagctcctct atcctagaca ccggtcgtca ccgccgaagc cgttccggag aggctctcca
     9901 taaaaaaggc ccaccagaat acgattacga cctggatacc cgaaagaaca ctatttgcgc
     9961 ccacgttaac cgtcgggccg aatgaccttc gcatggtgac tcactctcct tgcatctcct
    10021 acgctgacaa cgcgttgctc tgtgtcg
//
LOCUS       DBNGVD010000350         4794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4636149, whole genome shotgun sequence.
ACCESSION   DBNGVD010000350 DBNGVD010000000
VERSION     DBNGVD010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4636149"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16CM_09355"
     CDS             <1..202
                     /locus_tag="AC16CM_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3140076.1"
                     /translation="HRGVDKAYAIQAGREIRVMVQPSEVDDRIAAKLAYDISKQIETD
                     LEYPGQIKVTVIRESRVSEVAK"
     gene            278..1201
                     /locus_tag="AC16CM_09360"
     CDS             278..1201
                     /locus_tag="AC16CM_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140077.1"
                     /translation="MEGPYRDLGAGFARLTGVEGARRGPSRIGYVEDALLELLRNARD
                     AGARHVFVASTLARKRYRTLTVIDDGHGIPESHRDLVLEPGVTTRHLDPVTHPEDPPT
                     SPHGAGLSLYQIKVRSLSTRVLSASNPTSIQATFDTTVLPERALQSATRPSRTNLRAT
                     LLDFATPTIKTYYGPPARILATLLHKRIIQPDGGIEDLREAAEELGLLLSSRSIRRVL
                     RGEVLPEEPVTRDSEGPGSVVAREERARGRRVGGEPELRLGEEERAAIADILGRTARA
                     GYLEIEDLEFRSRPGEISITARVYEPEEEYE"
     gene            1194..2576
                     /locus_tag="AC16CM_09365"
     CDS             1194..2576
                     /locus_tag="AC16CM_09365"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR3140078.1"
                     /translation="MSSTTNEAPTAPIQLSRKPPGGPSVGPYPKPAESGGQGPPGAGK
                     TACIRTFGCQMNVHDSDRMRRMIQDAGYAEVQRYEEADLVILNTCYVRENAVDRVRGH
                     LGELSRLRREGRVKKVALTGCIGAAEEAQSLQKTFDIDLVLGTHNTYELAEFVGLPTM
                     RETYTPELPGTEGEHSAFVTIMTGCNYQCSYCIVPTVRGRMICRPLESVVGEIEDLVA
                     GGTRYVTLLGQTVDAWRYEGRRFCDLLEAVSEVAPRVWFTTSHPSNMEDRTLRAIGER
                     DTIVKRLHLPVQSGSDRMLKLMHRGYKADRYRRKISVFRDAAPEGTLSTDIIVGHPGE
                     TEEDHEQTLRLVEDCEFDSAYIFKFSPRRGTEAAALPGIVPDAIVQRRFLEVLRAVEQ
                     NAFRRNQRKVGGVEDIFVRHGATEDGKAIGETWSGHAVHVATEAAPGTYVRATIHSAG
                     PHVLYAQTTN"
     gene            2600..3451
                     /locus_tag="AC16CM_09370"
     CDS             2600..3451
                     /locus_tag="AC16CM_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140079.1"
                     /translation="MPECFVVCGPTASGKSELSDAWAEKLTETEGHHGPTAVIESMQV
                     YRELPRITNQARRRTAGLVGVVSVLGEWSVAAHRAGLRELVAGEDVFVLDAGTGMYLN
                     AMLLDFPLAPRVDPELRRRAGEASAGAENPRRAVRDRELALAGAEPRGSVWDGALRCR
                     TSMVYLRPPREVVDAAITVRSRRIVRDGPGEAEAVRAMLERGDNVNPSVLESVGVREL
                     LRYIEGEIPLDQAEELIATRTRRLARRQMRWFDKLARTLDGRASITVVDDGRHPSLHS
                     MHDIIWS"
     gene            3448..4722
                     /gene="hflX"
                     /locus_tag="AC16CM_09375"
     CDS             3448..4722
                     /gene="hflX"
                     /locus_tag="AC16CM_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3140080.1"
                     /translation="MNEERAVLVGAGETRQMAELGRLAGTLGIGVSAVLEQNRRDGTG
                     YLGRGKREELRDRVSEVDAMFVIADDELTASQARVLEKACGASVVDRTELIIRIFEAH
                     ARDAASRLEVELADLEYRLPRVKGKNPELSRLGGGGVTTRGPGEQQLEYDRRVIRDRI
                     TTINRKLKDERSGRAVRGARLKESGTPTVALVGYTNAGKTTILNRLSGANRSTRDRLF
                     ETLETTTRLVEGTGADGADGSDGASRPDFVVTDTVGFIRKLPTQLVHSFASTLEASGD
                     ADVRVLCADASSEDLEDEISTVTETLNESFPSENGTSPDSGSTILCLNKTDLIDEDRT
                     RKLRSEYPEAVMISALHDCDELLQRIYAAISSRRQRMYLLIPHSDYAAASHLYGVAEI
                     HARENTQEGVLMDVSLPNSIAARYLQYTTTSA"
BASE COUNT          980 a         1519 c         1560 g          735 t
ORIGIN      
        1 ccacaggggc gtggacaaag cctacgccat ccaggccggg cgtgagatcc gcgtcatggt
       61 ccagccctca gaggtcgacg accgcatcgc cgccaagctc gcgtacgaca tctccaagca
      121 gatagagacc gatctggagt acccgggcca gatcaaggta accgtaatcc gcgagagccg
      181 cgtgagcgag gtcgccaagt aagatggtga cgtctgcgcg accaccctct aggggcggtt
      241 cgcggaccgc ccagccacct gacgcccgac cctgacggtg gagggccctt acagagacct
      301 cggcgcaggc ttcgcccgcc tgaccggggt cgagggcgcc cgccgcggcc cctcgcgcat
      361 aggatacgta gaggacgccc tcctggagct actgcgcaac gcccgcgacg ctggtgccag
      421 gcacgtcttc gtagcttcga ccctcgccag gaaacgctac aggaccctca cggtgataga
      481 cgatggccac ggtatccccg aatcgcacag ggatctcgtc ctggagccgg gcgtaacgac
      541 ccgtcacctc gatcccgtaa cccaccccga ggacccaccg acctcgcccc acggcgccgg
      601 cctctccctc taccagataa aggtgagatc cctcagcacc agggtcctct ccgcctcgaa
      661 cccaacctct attcaggcaa ccttcgacac aacggtcttg cccgaacggg ccctccagtc
      721 cgccacccga ccttcgagga ccaacctgag ggccaccctc cttgacttcg ccacccccac
      781 gataaaaacc tactacggcc ccccagcccg gatcctggcc accctcctac ataaacgcat
      841 aatacagcca gacggtggga tcgaggattt gcgggaggct gcggaggagc taggcctgtt
      901 gctctcctct aggagcatac ggcgggtttt gcggggcgag gtattgccgg aagagccggt
      961 cacgagggat agcgaggggc ctggctctgt cgtcgcgcgg gaggagcgcg cacgcggcag
     1021 gcgagtcggg ggggaaccgg agctcaggct cggagaggag gaaagagcgg ccatcgcgga
     1081 tatactaggg cggacggccc gtgccgggta cctcgaaata gaggacctcg agttccggag
     1141 ccgtccgggg gagatctcca tcacagcccg cgtctacgag ccggaggaag agtatgagta
     1201 gcacgaccaa cgaggcgcca accgccccga tacagctctc ccgtaagccc ccgggcgggc
     1261 cgtctgtggg gccctacccg aagcccgccg aaagcggggg tcagggccca ccgggcgccg
     1321 gcaagaccgc ctgcataagg accttcgggt gccagatgaa cgtgcacgac tccgaccgga
     1381 tgcggcgcat gatacaggat gcgggctacg ccgaggtcca gcgctacgag gaggcagacc
     1441 tcgtcatcct caacacctgc tacgtccgcg agaacgccgt agaccgggta agggggcacc
     1501 tgggcgagct cagccgactg cgccgcgagg gccgcgtcaa gaaggtcgcc ctgaccggct
     1561 gcataggcgc cgcagaagaa gcccagagcc tgcagaagac cttcgacata gacctcgttc
     1621 tgggcacgca taatacttac gagctggcag agttcgtggg gctgccgacg atgagagaga
     1681 cgtacacgcc ggagttgccc ggtacagagg gggagcacag cgccttcgtc accataatga
     1741 cggggtgcaa ctaccagtgc agttactgta tcgtgccgac ggtcaggggg cgcatgatct
     1801 gccgcccgtt agagagcgtg gtcggggaga tcgaggacct cgttgccggc ggtacgcgct
     1861 acgtgacgct cctcgggcag accgttgacg cgtggaggta cgaaggacgt cgcttctgcg
     1921 accttctgga ggccgtctcc gaggtcgcgc cgcgcgtgtg gttcacgaca agccacccat
     1981 caaacatgga agacaggacg ctcagggcta tcggggagcg ggacaccatc gtcaagcggc
     2041 tccatctgcc tgtccagtcg ggctctgaca ggatgctcaa gcttatgcac cgcggctaca
     2101 aggcggatag gtaccggcga aagatctcgg tcttccggga tgccgcgccg gaggggaccc
     2161 tgagcactga catcatcgtg ggccatcccg gcgagaccga agaggaccac gagcagacgc
     2221 tccggctcgt cgaggactgc gagttcgact cggcctacat cttcaagttc tcccccagac
     2281 gaggtacaga ggccgccgcc ctccccggta tcgtccccga cgccatcgta cagcgccgct
     2341 tcctggaggt gctgagggcc gtcgagcaga acgcgtttag gagaaaccag cgcaaggtag
     2401 gcggtgttga ggacatcttc gtccgtcacg gcgccacgga ggatggcaag gccatcggcg
     2461 agacctggag cggccacgcc gtccacgtcg ccaccgaagc cgcacccggc acctatgtca
     2521 gggcgaccat ccattccgcg ggaccccacg tgctgtacgc ccagacaacc aactaggtac
     2581 cacgaaggcg gcgtctcaca tgccagagtg tttcgtcgtc tgcgggccca cagccagcgg
     2641 gaagagcgag ctctccgatg catgggccga gaagctcacg gagacagagg gacatcacgg
     2701 tccaacggcc gtaatagagt ccatgcaggt gtaccgagaa ctacccagaa taaccaacca
     2761 ggcaaggcgc agaaccgccg ggcttgttgg ggttgtctcg gtgcttggtg agtggagcgt
     2821 ggcggcacac agggccggct tgagggagct ggtagccggg gaggacgttt tcgtgctaga
     2881 cgcgggtacg gggatgtatc tgaacgccat gctcctggat tttccgctag cccctcgggt
     2941 ggaccccgag ctgcgcaggc gtgccgggga ggcttcggcg ggcgctgaga acccaaggag
     3001 ggccgtgcgg gatcgggagc tggcgctcgc tggtgccgag cccagaggct cggtgtggga
     3061 cggagctctg agatgccgga cgagcatggt ctatcttcgc cctccccgag aggtggtaga
     3121 cgcggcgatc acggtccggt cgcgcaggat cgtccgcgac gggcctggag aggcggaggc
     3181 cgtcagggcc atgctagagc gaggcgacaa cgtcaacccc tccgtgctcg agtctgtcgg
     3241 cgtgcgcgag ctcttgcggt atatagaggg ggagatccca ctcgatcagg cagaggagct
     3301 gatagccacc cgcacccgcc gtctggcccg gcgtcagatg cgctggttcg acaaactcgc
     3361 gagaacgctc gatggacggg cgtccatcac cgtcgttgac gacggccgcc atcccagttt
     3421 gcattctatg catgatataa tctggtcatg aacgaggaga gagcggtact ggtaggggcg
     3481 ggggagactc gtcagatggc cgagctgggg cgtcttgcgg ggacgctagg cataggggtg
     3541 tccgcggtgc tggagcagaa ccgcagggat ggcacggggt atctcggccg tggcaagcgc
     3601 gaggaattgc gggaccgtgt ctcggaggta gacgctatgt tcgtcatagc cgacgacgag
     3661 ttgacggcct cccaggccag ggtgctcgag aaagcgtgcg gggcctcggt agtagacagg
     3721 acggagttga tcatccgcat tttcgaggcg cacgccaggg atgccgcgag ccgcctcgag
     3781 gttgagctcg ccgacctcga atacaggctt cccagggtca agggcaagaa tcccgagcta
     3841 agccgcctgg gcggtggcgg ggtcacaacc aggggacccg gagagcagca gctagagtac
     3901 gacaggcgtg tgataaggga ccgcatcacg accatcaacc gaaagctgaa ggacgagagg
     3961 tccggcaggg cggtaagggg cgcgcgcctc aaggagtcag gtacgccgac cgtcgccctg
     4021 gtcggctaca caaacgctgg caagaccacc atcttgaaca ggctctccgg tgcaaaccgc
     4081 tctaccaggg acaggctctt cgagacgcta gagacgacca cccgcctcgt cgagggtacc
     4141 ggggcagatg gcgctgacgg ctcggatgga gcctcgcgtc ctgactttgt ggtaaccgat
     4201 acggtcggct tcataagaaa gctgccaacc cagctcgtgc actccttcgc ctcgacactc
     4261 gaagcctccg gcgacgccga cgtcagggtg ctctgcgccg acgcctcaag cgaagacctg
     4321 gaggatgaga tctccacggt cacagagacg ttgaacgaga gcttcccctc cgagaacggc
     4381 acttccccgg actccggctc aaccatcctg tgcctgaaca agacggacct gatcgacgaa
     4441 gatcggaccc gcaagctccg ctcggagtac ccagaggccg ttatgataag cgccctccac
     4501 gactgcgacg agctcctgca gcgcatctac gccgccatct cctccaggag gcagaggatg
     4561 taccttctga tccctcactc agattacgcc gccgcgagcc acctctacgg cgtcgccgag
     4621 atccacgccc gcgaaaatac ccaggaaggc gtgctcatgg atgtcagcct tccaaactct
     4681 atcgccgccc gctacctcca gtacacaacg acttccgcat aatacatctc gaaggtcgta
     4741 gaatatcgtg gatgacatgt acgccaacct gaggtttcca aggggaagac taaa
//
LOCUS       DBNGVD010000351         2879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2449726, whole genome shotgun sequence.
ACCESSION   DBNGVD010000351 DBNGVD010000000
VERSION     DBNGVD010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2449726"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16CM_09380"
     CDS             complement(<1..707)
                     /locus_tag="AC16CM_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140081.1"
                     /translation="MARGAASNKSFDTSTVTSWVRGRGVYVAFVVLVVFNLIVTPNFA
                     SVATLTTLLITVSPIILVSLGVALAIGTEGIDLSVGSVMALASAFLPLYLGYGWPVGL
                     SIALLVGVLAGLVNGSLVAFVGVQPIIATLAMLVGGRGLAQIFAGGQSLPVTDPVILW
                     LGQGRLFGIPAPVIVAAVAVLVVGFLVRRTTFGRYVVAIGGNRSASYLSGHPVRRTLL
                     AVYVISGLLAALAGVVAT"
     gene            complement(700..2262)
                     /locus_tag="AC16CM_09385"
     CDS             complement(700..2262)
                     /locus_tag="AC16CM_09385"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010061739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3140082.1"
                     /translation="MERRSPVLEVRRVTKRFPGVLALDGVSFELRPGEAHALVGENGA
                     GKSTLIKVITGVYRPDEGEILFEGEEVSFAEPRESQAAGISTIYQEINLIPLLSVAQN
                     VFLGREPRNALGMIDKARMNRDAAEILERYGIRADVTAPLRSLSLGVQQMVAIARAIS
                     VDARAVIMDEPTSSLEAREVETLFGVIGQLREEGVGVVYVSHRLEELYEICNRVTVLR
                     DGRVAHTGELAELSRLQLIAHMLGRELSEVQKEGATGFGGEHETAREPVLEAQDLSQH
                     PRLREVSFDVHPGEVVGLAGLLGAGRTETAKAIFGAEPIDSGSVRVEGQDIKRGSPGA
                     AIKAGIAFLPEDRKLEGIIPDLSVRENIVAAALPRLARAGLVSEKAQDELVDEFIKSL
                     GIKASSPNQPVRELSGGNQQKVLLARWLCMNPKVLILDEPTRGIDVGAKAEIQMVVDD
                     LAAKGLGIILISSELDEITEGSDRVVALRDGAVVGLLSGEEITEGNLMTMLAHGEGLG
                     EGDQKTGEDADG"
     gene            complement(2280..>2879)
                     /locus_tag="AC16CM_09390"
     CDS             complement(2280..>2879)
                     /locus_tag="AC16CM_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3140083.1"
                     /translation="DRETAGKPCEDYITFMGSNFFEQGERAADIMADLTNEKGKVAVL
                     EGTPGASVTIDRTEGFEKQLKEYPEMEIVASQTGEFLRTKGQTVMEQLIQSNPDITAV
                     YAENDEMALGAIQALKDAGKDPGQDVKVVSIDGTQQAVQAITTGDINAVIETNPRFGP
                     LAFDTFDQFLAGEPIPQKIIVQDDVYTEKNAQEKLDETY"
BASE COUNT          478 a         1007 c          887 g          507 t
ORIGIN      
        1 gtggccacga ccccggccag cgcggccaag agaccactga taacgtacac cgcgaggagc
       61 gtcctgcgca ccgggtggcc cgacaggtag ctcgccgagc gattgccccc gatggccacc
      121 acgtacctgc cgaacgtggt gcgccttacc aggaaaccca ccaccaggac cgccacggcc
      181 gccacgatga ccggcgccgg tatgccgaag aggcgtccct gtccgagcca caggatcacc
      241 ggatcggtga ccggcaacga ctggcccccg gcaaagatct gggccagtcc cctaccgccc
      301 accagcatgg ccagcgtggc gatgatgggc tgcaccccga cgaaggccac gaggctgccg
      361 ttcacgagcc cggccagaac gcccaccagg agggcgatcg acagcccgac cggccagccg
      421 tacccgaggt acaacgggag aaacgccgag gcgagggcca taaccgaccc gacggagagg
      481 tctatgcctt ccgtccctat cgccagcgcc acgcccagag agacgaggat tatgggcgag
      541 accgtgatca gcagggtggt gagcgtcgcc accgaggcga agttgggggt gactatgagg
      601 ttgaagacca ccagcaccac gaaggccacg tacaccccac ggccgcggac ccaggaggtc
      661 accgtagagg tgtcgaaaga cttgttcgac gccgctcccc tagccatctg cgtcctctcc
      721 ggtcttctgg tccccctcgc ccaaaccttc cccgtgcgcg agcatggtca tcaggttgcc
      781 ctcggtgatc tcctcccccg agagcaaccc caccacggcg ccgtccctca acgcgaccac
      841 gcgatcgctg ccctcggtga tctcgtccag ctccgaagag atgaggatga tgcccagccc
      901 cttcgccgcg agatcgtcca cgaccatctg tatctccgct ttcgctccca cgtcgatacc
      961 gcgggtaggc tcgtccagta tgagcacctt ggggttcatg cacaaccagc gggccagaag
     1021 gaccttctgc tggttgccgc cggagagctc cctgaccggc tggtttgggc tcgaagcctt
     1081 gataccgagg ctctttatga actcgtcgac caactcgtcc tgcgccttct ccgaaaccag
     1141 cccagcccgt gccaacctcg gcagggccgc ggcgacgatg ttctcccgca ccgagagatc
     1201 gggaataatg ccctccagct tgcggtcctc gggcaagaag gcgattccag cctttatcgc
     1261 cgcgccgggg gagcccctct tgatgtcctg cccctctacc ctgacggacc cggagtcgat
     1321 cggctcggcc ccgaagatcg ccttggccgt ctcggttctg ccggcaccga ggaggccggc
     1381 gagccccacc acctcccccg gatggacgtc gaaggagacc tcgcgcaggc gtgggtgttg
     1441 gctgaggtcc tgcgcctcga gaacgggttc cctggcggtt tcgtgctccc cgccgaagcc
     1501 ggtagcaccc tccttctgaa cctcggagag ctctcggcct agcatgtggg cgatgagttg
     1561 caggcgggag agctcggcga gttccccggt gtgcgccacc cgcccgtcac gcaggaccgt
     1621 cacgcggttg cagatctcgt agagttcctc gaggcggtgg cttacgtaga cgacgccaac
     1681 cccctcctcg cgcagctgtc cgatcacccc gaagagtgtc tccacctccc gcgcctcgag
     1741 cgacgaggtg ggctcgtcca tgatgacggc cctcgcgtcc accgagatgg cccgcgcgat
     1801 ggcgaccatc tgctgaacgc ccaggctcag ggagcgcagg ggagcggtga cgtccgcgcg
     1861 aatgccgtaa cgctccagga tctcggccgc gtcccggttc atgcgagcct tgtcgatcat
     1921 gcccagagcg ttgcggggct cgcgccccag gaacacgttc tgggccacgc tgaggagcgg
     1981 gatcaggttg atctcctggt agatggtgct gatacccgcc gcttgcgact cgcgcggttc
     2041 ggcgaaagag acctcttcgc cttcgaacaa gatctcgccc tcatcgggcc tgtagacccc
     2101 ggtgatgact ttgataaggg tggatttccc ggcgccgttc tcgccgacca gggcgtgcgc
     2161 ctctcccggc cgcagctcga aagagacgcc gtcgagggcc agtacgccgg ggaaccgttt
     2221 cgtcacccgg cgcacctcga ggacggggga gcgtctctcc atcgtagtgc tccctccccc
     2281 tagtaggtct cgtccagttt ttcctgagcg ttcttctcgg tatacacgtc gtcctggacg
     2341 ataatcttct gcgggatggg ctccccggcc aggaactggt cgaaggtgtc gaaggccagc
     2401 gggccgaagc gcgggttggt ctcgatcacc gcgttgatgt cacccgtagt tatcgcctgg
     2461 acggcctgct gggtgccgtc gatggagacc accttgacgt cctgcccggg gtccttcccg
     2521 gcgtccttga gggcctgtat ggccccgagc gccatctcgt cgttctccgc gtagaccgcg
     2581 gttatgtccg ggttcgactg gatgagctgt tccatgaccg tctggccctt ggtcctcagg
     2641 aactccccgg tctgggaggc gacgatctcc atctcgggat actccttgag ctgcttctcg
     2701 aagccctcgg tgcggtcgat cgtgaccgag gcccctggcg tcccctcgag cacggcgacc
     2761 ttgcccttct cattggtgag gtcggccatg atatcggccg cccgctcgcc ctgctcgaag
     2821 aagttcgagc ccatgaacgt gatgtagtcc tcgcaaggct tccccgccgt ctcgcggtc
//
LOCUS       DBNGVD010000352         6136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3906924, whole genome shotgun sequence.
ACCESSION   DBNGVD010000352 DBNGVD010000000
VERSION     DBNGVD010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3906924"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="AC16CM_09395"
     CDS             <1..2200
                     /locus_tag="AC16CM_09395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3140084.1"
                     /translation="DLAPTPRAAAPAAPRSGVRLRGRGLVAARISWFAVAVLSTVVFV
                     AGVPYQFSVLMGARRDADVALSGIPDGVLRSFLEGWVFTGAYPYLALGIEACLAVSMS
                     VVAVFIYWQMSDDRLAWLASVTLFSYAAVWTGFGEALAAAWPPWWAAAGLLEATGLAF
                     SITLFFAFPDGRFVPPWTRYVAVVWVGWSIYWTLGPLTLPGLFPLPLEQEFFLLSTFP
                     TLAFNLLAIAVQVYRYRRVSSDDERRRTRWVVLSFAVGTAVYFAVNAARIALSALGEA
                     RVAGFLHHFVGVPLVQLSLLLIPLCVLLAIVRRRLFGIEVIVNRALVYAALTACVIGI
                     YVLVVGYLGALLRTGSTLPASLVATGIVAVLFQPLRDRLQRAANRMVYGDRDEPYAVL
                     SRLGQRLEATLAPGSVLPTIVETVVEGMKVPHAEIFIVRDGSFESAARCGVPTGDPLV
                     LLLSYQNETVGRIEVSPRARDEPFAPADLRLLQDLARQAGVAVHAVRLTDDLQRSRER
                     MVIAREEERLRLRRDLHDGVGPQLAALTLKIETARNKLIDEPGADVLLSDLAERARDA
                     VADVRRSVHALRPPALDELGLVPALRETAAQHSQNGLSIFVEVPGDLAPLSAAVEVAA
                     YRIAQEAITNVVRHANARNCEVRFDLDEASRSLTLEVTDDGRGIGKHRGLGVGLYSMR
                     ERAEELGGSCIIEDMPSGGTRVNARLPLSDGALRSENHPTTWYQERGADR"
     gene            2197..2859
                     /locus_tag="AC16CM_09400"
     CDS             2197..2859
                     /locus_tag="AC16CM_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140085.1"
                     /translation="MTPERPKIRVLITDDHPLFRDGMHGLLDSVEGTEVVGEADNGEV
                     AVELAGTLQPDVILMDVKMPGMSGIEATREILERNPQVGILVVTMLEDDDSVFAAMRA
                     GARGYLLKGAHQEEVLRAIRAVANGEAIFGPGIARRVMGLFSSAKPQAPRAFPELTDR
                     EQEILRLIADGRGNAEISSDLFLSLKTVQNHVSNIFRKLQVADRAQAVIRAREAGLGG
                     GP"
     gene            2880..3353
                     /locus_tag="AC16CM_09405"
     CDS             2880..3353
                     /locus_tag="AC16CM_09405"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR3140086.1"
                     /translation="MVRILFVCTGNICRSPMAQGIFEDVALREGLADEISVDSAGTHG
                     FYTAGEPPDPRAQESLLSRGIDISAQRARLLCPEDCGEFDYILTMDEANYRATSALCG
                     GNPIVRRFLDYAPGAATEVRDPYLGGAQGFQYALELIEGASEGLLREIQARRPGG"
     gene            3346..4206
                     /locus_tag="AC16CM_09410"
     CDS             3346..4206
                     /locus_tag="AC16CM_09410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR3140087.1"
                     /translation="MAERIIASGVEAALGERLRGFCPLGGGCIGEVYRVELGDGTPLV
                     AKVDASGEAHLEREAYMLRYLREKSELPVPEVYHGTETLLLMEFVEGSSLFSTEAERH
                     AAELLAALHEITADTYGHERNTLIGSLDQPNPRTGDWVEFFRDQRLLYITRVAYEAGR
                     LPGGEVRRLQRLSERLDEIIDMPNPPALIHGDVWSANVLARDDRIAAFLDPALYYADP
                     EMELSFISLFDSFGAAFFERYEEIRGIDERFYGARRDLYSLYPLLVHVYYFGGGYLQS
                     VRNTLDRFGV"
     gene            complement(4428..5231)
                     /locus_tag="AC16CM_09415"
     CDS             complement(4428..5231)
                     /locus_tag="AC16CM_09415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140088.1"
                     /translation="MAEERTKLRAVFDEAALLYDEARPGYPEELFQDVVSLSGIPEGG
                     RILEIGCGTGQATVPLARRGYRILCVELGENLAAVSRRNLDAYPRAEVHVGPFEEWQP
                     QGASFDLAISATAFHWLDHEVAYPRVADALGEAGSLALFWSVHVHTEADGGIFQEVQK
                     VYARETPEIVGPEDHKGPPRPEDVPSRTPEIERSGRFEVTATRTYRWDETYDTSGYLS
                     VLNTYSGHRSLGKHTHDRLFRGIASVIDQRFDGSITKGYLTTLYVARKR"
     gene            complement(5241..5711)
                     /locus_tag="AC16CM_09420"
     CDS             complement(5241..5711)
                     /locus_tag="AC16CM_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HIR3140089.1"
                     /translation="MSVGKALALGAISGLRSASGPAFVGRAASRGDMDLEGTRLAFLG
                     RPLLSKALTLAQLGEMVGDQLSVTPSRTGWSPLLGRAVSGGLVGAAVFLSKGGRATTG
                     AILGSSAAVAAAFAGESLRSLVVSASGLPDAGVAVAEDAVVLVTGSQATPKGVR"
     gene            complement(5708..>6136)
                     /locus_tag="AC16CM_09425"
     CDS             complement(5708..>6136)
                     /locus_tag="AC16CM_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3140090.1"
                     /translation="LLTTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDE
                     EENPETVMAEAPHGTAPSLQGKNVANPMAMILAGAALLTFFHEEDASRVSRAIYEATF
                     ETILDGVKTADLGGSAGTDEFTNEIIRHVRTKLEVWSALA"
BASE COUNT         1004 a         1942 c         2040 g         1150 t
ORIGIN      
        1 cgacctggcg ccaacacccc gggccgcggc ccccgcggca ccgcgttccg gcgtgcggtt
       61 gcgggggcgg ggactcgttg ccgccaggat ctcgtggttt gcggtggccg tgctctcgac
      121 cgtcgtcttc gtcgcaggtg ttccctacca gttctctgtt ttgatggggg ctcggaggga
      181 cgcggacgtg gcactctcgg gcattcccga cggcgtgctg cgctcctttc tggaaggatg
      241 ggtctttacg ggggcctatc cgtatctcgc cctcggaatc gaggcgtgcc tcgctgtctc
      301 gatgtccgtg gtcgcggtct ttatttactg gcagatgtcc gacgaccggc tggcttggct
      361 ggcctcggtc acgctcttct cctacgcggc ggtgtggacg ggcttcggcg aggccctggc
      421 ggccgcgtgg ccgccgtggt gggcggcggc tggcctgctg gaggcgaccg gcctagcctt
      481 ctccatcacg ctgttcttcg ccttcccgga cggccgcttc gtacccccct ggacacgcta
      541 cgtggccgtg gtctgggtgg ggtggtccat ctactggacc ctcgggccgc tgacgttgcc
      601 tggcctcttc ccgctgccgc tggagcagga gttctttttg ctctcgacgt tccccacgct
      661 cgccttcaac ctcctggcga tcgctgtgca ggtttacagg tacagaaggg tctcctcgga
      721 cgatgagcgc cggcggacgc gctgggtcgt cctctcgttc gccgtgggaa ccgccgtgta
      781 cttcgcggtc aacgctgcca ggatcgcgct ttccgcgctc ggcgaagcac gggtggccgg
      841 gtttctgcat cacttcgttg gcgtccccct ggtccagttg tccttgctct tgatacctct
      901 ctgtgtgttg ctcgcgatcg tccgtaggcg gctcttcggc atcgaggtca tcgtaaaccg
      961 cgctctggtc tacgcggcgc tgaccgcctg tgtcatcggt atctacgtgc tcgtggttgg
     1021 ctacctcggg gcattgttac gcacggggtc gaccttgccc gcaagcctgg tggcgacggg
     1081 gatagtggcc gtgctcttcc agcccctgcg agatcgtttg caacgggcgg ccaaccgcat
     1141 ggtgtacggt gatcgggacg agccctatgc cgtgctctcg aggctcgggc aacgtctcga
     1201 ggcgacgctc gccccgggct ctgtgttgcc gaccatagtc gagacggtcg tcgaggggat
     1261 gaaggttccg cacgcggaga tctttatcgt gcgtgatggc agcttcgaga gcgccgcccg
     1321 atgcggcgtt cccacaggcg atccgctcgt gctgctcctc tcgtaccaga acgagaccgt
     1381 ggggcgcatc gaggtctcgc cccgcgcgag ggacgagccg ttcgcgccgg cggacctgcg
     1441 tctcttgcaa gacctggcca gacaggccgg cgtggccgtc cacgccgtcc gtctaacgga
     1501 tgacctgcaa aggtccaggg aaaggatggt tatcgcccgc gaagaagaac ggttgcgact
     1561 caggcgcgac ctgcacgatg gcgtgggccc gcaactcgcc gcgctcacgc taaagatcga
     1621 gacggctcgc aacaagctga tcgacgaacc aggggccgac gtcttgctct ccgacctggc
     1681 cgagcgcgcc cgcgatgccg ttgccgacgt acggcgctcg gtccacgccc tgaggccgcc
     1741 cgcgctcgac gaactcggcc tcgttccggc cctgcgcgag accgcggccc agcacagcca
     1801 gaacggcctg agcatcttcg tcgaggtgcc gggagatctc gccccgttgt cggccgccgt
     1861 agaggtggcc gcgtaccgca tcgcgcagga ggccattacc aacgtggtac ggcacgccaa
     1921 cgcgcgcaac tgcgaagtac gcttcgacct cgacgaggca tctcgttcgc tgacactgga
     1981 ggtaacggac gacgggaggg ggatcggaaa gcaccgcgga ctcggtgtgg gactatattc
     2041 catgcgcgag cgggctgagg aactaggtgg ctcatgcatc atcgaagata tgccctcggg
     2101 tggaacccgc gtcaatgcca ggctccccct atcggatgga gcattgcgct ccgagaatca
     2161 tccaacgacc tggtatcagg agaggggagc ggatcgttga cgcctgaacg gccgaagatt
     2221 cgggttctta tcaccgacga ccacccgctt tttcgtgacg gtatgcacgg tctcctcgac
     2281 tcggtcgagg ggaccgaggt ggtgggcgag gccgacaatg gggaggtggc cgtcgagctt
     2341 gccgggacgc tccaacccga cgtgatcctg atggacgtca agatgccagg gatgagcggt
     2401 atcgaggcga cgcgggagat cctggagagg aaccctcagg tcgggatcct ggtcgtgacg
     2461 atgctcgagg acgacgactc ggttttcgcc gcgatgcggg ccggggcacg gggatacctg
     2521 ctgaagggag cccatcagga ggaggttctg cgcgctatca gggctgtagc aaacggtgag
     2581 gcgatcttcg gtccgggaat cgccaggagg gtgatgggtc tgttctcctc cgcgaaacca
     2641 caagctcctc gcgctttccc cgagttgacc gaccgtgagc aagagatact caggctcata
     2701 gcagacggtc ggggtaacgc ggagatctca tccgatctct tcctgagcct gaagacggta
     2761 cagaaccacg tctccaatat cttcagaaag ctacaggtgg ccgacagggc ccaggctgtt
     2821 atccgcgccc gagaggcggg actcggcggc ggcccgtagc gtgtcataga atcccacgca
     2881 tggttcgtat acttttcgtc tgtacgggga acatctgccg tagccctatg gcccagggta
     2941 tcttcgagga cgtcgcgctg cgcgagggcc tggccgacga gataagcgtg gactccgccg
     3001 gcacccacgg cttctacaca gctggggagc cgccagaccc aagggcgcaa gagagcttgc
     3061 tctcccgtgg tatagacatc tcggcccaga gggcgcgcct gctctgcccc gaagattgcg
     3121 gggagttcga ctacattctg accatggacg aggcgaacta ccgcgcaacc tcggcgttgt
     3181 gcggaggaaa tcccatcgtg agacgctttc tcgattacgc accgggcgcc gcaaccgagg
     3241 ttcgggatcc ctatctcggc ggtgcccagg ggttccagta cgccctcgag cttatcgaag
     3301 gagcctccga ggggctgctg cgggagatcc aggccagacg tccaggtggc tgagaggatc
     3361 atcgcctccg gtgtcgaggc cgccctgggc gagcgcctgc gcggcttctg cccgctgggc
     3421 ggtggttgca tcggcgaggt ctacagggtc gagctcgggg acggcacgcc gctcgtcgcc
     3481 aaggtcgacg cttccggcga ggcgcacctg gagcgcgagg cgtacatgct tcgctacctg
     3541 cgagagaaga gcgagcttcc ggtcccggag gtctaccatg gcaccgaaac actgctgctt
     3601 atggagttcg tagaaggaag tagcctcttc tcgacagaag ccgagcggca cgccgccgag
     3661 cttctcgcag cgctgcacga aataactgcc gacacctatg gccacgagcg aaacaccctc
     3721 atcggcagcc tcgaccagcc caacccacgg accggagact gggtcgagtt cttccgggat
     3781 cagaggctcc tgtacattac gcgggttgca tatgaggcgg gcaggcttcc tggtggggag
     3841 gtgcgcaggc tgcagcggtt gtccgagagg ctcgacgaga ttatcgacat gcccaacccc
     3901 ccggccctca tccatggcga cgtctggagc gccaacgtgc tggccaggga tgaccgaata
     3961 gccgcgtttc tagatccagc gctgtactac gcggaccccg agatggagct atcctttatc
     4021 agcctcttcg actcctttgg cgcagcgttc ttcgagcgct acgaggagat aaggggcata
     4081 gacgaacgtt tctacggagc gcgccgcgac ctgtatagcc tgtacccgtt gcttgtccac
     4141 gtgtactact tcggcggcgg atacctgcaa tcggtcagga ataccctgga ccgtttcggg
     4201 gtgtagtcaa aggtcaggtc gctgcggcgc atgacaacga tcccgtgcat gtggtcgggc
     4261 actatcatga aggcgtccaa cgcaaacacc ccaggacgcg agcaatcgta tcccccggat
     4321 cgaacactgc gtcggcccat cgctccggac gcgagcctta cgagactttc tctgcggccc
     4381 ataccttgtt atcggtatgt ccctggcaac ctcgagatga gggccttcta tctcttgcgg
     4441 gccacgtaca gagttgtcag gtaacccttc gtgatgctgc cgtcgaaccg ctggtctata
     4501 acgctggcga ttcctcggaa gagacggtcg tgggtgtgct tgccgaggct tctgtgaccg
     4561 gagtaggtgt tcagcacgct cagatagcct gaggtgtcgt aggtttcgtc ccagcggtag
     4621 gttctggtcg ccgttacctc gaaccggcca gacctctcga tctcgggggt tctgcttgga
     4681 acgtcttctg gtcggggagg tcccttgtgg tcttcggggc cgacgatctc gggggtctct
     4741 cgagcgtaga ccttctgcac ctcttggaag atgccaccat cggcctccgt gtgcacgtgc
     4801 acgctccaga acagggcgag ggaacccgcc tctccaagcg catcggcgac tctgggatag
     4861 gcgacctcgt gatccagcca gtgaaaggcg gtggctgata tggccaggtc gaaggacgct
     4921 ccttgcggct gccactcctc gaaaggaccc acgtgtactt cggcccgcgg gtacgcatcg
     4981 aggtttcggc gcgagaccgc ggccaggttc tccccgagct cgacgcagag tatccggtag
     5041 ccgcgtcgcg cgaggggcac cgtggcctgg ccggtgccgc aaccgatctc gagaatcctg
     5101 cccccctccg gtatgccgga gagcgagacc acatcttgga agagctcttc cgggtagccg
     5161 ggcctcgcct catcgtagag caatgccgcc tcgtcgaaga cggcccggag cttcgtcctc
     5221 tcctctgcca tgagcgcggt ctagcgcacc cccttcgggg tcgcctggga tcccgtaacg
     5281 agtacaacgg cgtcctccgc gaccgcgacg cctgcgtctg ggagtccgga agcggagacc
     5341 acgagcgagc gcaggctctc gcccgcgaac gccgcggcga cggcggcaga ggagccgagg
     5401 atggcgccgg tcgtggcgcg acctcccttc gagaggaaca cggccgctcc caccaggccg
     5461 cccgagaccg cgcgtccgag gaggggcgac cagcccgtgc ggctcggcgt caccgacagc
     5521 tggtccccga ccatctcccc gagctgggcc agggtgagcg ccttggatag gagtggacgg
     5581 cccaggaagg cgagacgagt gccttcgagg tccatgtcgc cgcggctcgc cgcgcggccg
     5641 acgaaggccg gtcccgacgc ggatcgcagg cccgagatcg cgcccagggc cagggctttt
     5701 ccgacgctca tgccagcgcg ctccacacct cgagcttggt tctgacgtgg cgtatgatct
     5761 cattcgtaaa ctcgtccgtg ccggcggagc ccccgaggtc ggcggtcttg acgccgtcga
     5821 ggatggtctc gaaggtcgcc tcgtagatgg cccgcgagac gcggctggcg tcctcttcgt
     5881 ggaagaaggt tagaagggca gccccggcca ggatcatggc catagggttg gcgacgtttt
     5941 ttccttggag gctgggcgcc gtgccgtgcg gggcctcggc catgaccgtc tcggggttct
     6001 cttcctcatc cagcgagatc aggagcgact ccgccccggc gatggagccg aacatctgca
     6061 tgaccaggtc agagatgcag tcgccgtcgc ggttgagcgt gggtatgacg agcggctcgc
     6121 cgtaggtagt gagcag
//
LOCUS       DBNGVD010000353         1647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_627166, whole genome shotgun sequence.
ACCESSION   DBNGVD010000353 DBNGVD010000000
VERSION     DBNGVD010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_627166"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="AC16CM_09430"
     CDS             complement(<1..692)
                     /locus_tag="AC16CM_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140091.1"
                     /translation="MNHDGNVDQRSRRREQRGWYFYDWANSAFFTTVITVFLGPYLTA
                     VTENAADASGLVYPFGIPILAGSYFPYVLSLSVGLQVFLLPLTGAIADRTGRKKRLLM
                     LFAYVGAVSTMGLYLVQGSAYLLGGFLFVIANLSAGASLVVYNAFLPEISGPDERDRV
                     SSRAWALGYLGGGLLLAANLALFSAHESLGLSEGHAVRICLLSAGAWWALFATIPLAT
                     LRDRRARHSEERG"
     gene            complement(689..>1647)
                     /locus_tag="AC16CM_09435"
     CDS             complement(689..>1647)
                     /locus_tag="AC16CM_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3140092.1"
                     /translation="DQALLRNPAARSVWSLLAERFPEKRLAPDTSPQLDLGIDSLGWV
                     NLTLEIGERIGVEIGEAAIGRIDTLRDLLSEVALGTGRTAHQASPLERPEEVLDEKQM
                     RWLRPLGRVASALAGGMFSLNRAIMRSLFRLRAEGLENLPEEGPFIVAPNHVSYLDAF
                     VVAAALPRDVRRRTYWAGWTGAAFGNPLTRLISRLAQVVPVDPERAGFSSLAYGAAVL
                     ARGQNLVWFAEGERSRTGSLQQFKPGVGMILDHHPVPVVPVFIRGTYEAMPRGQLLRR
                     LERVSVTFGEPFDPRGPDGNVEPREQVVEALHGRVFELGQRG"
BASE COUNT          317 a          553 c          525 g          252 t
ORIGIN      
        1 cccctctcct cggaatgcct cgccctccgg tccctgaggg tggctagcgg tatcgtggcg
       61 aaaagcgccc accacgcacc ggccgaaagc aggcagatac gcacggcatg cccttccgag
      121 aggccgagcg actcatgggc ggagaacagc gccaggttcg ccgcgaggag caaaccgcct
      181 ccgaggtagc cgagtgccca ggcgcgagag gagactcggt cgcgctcatc agggccggaa
      241 atctcgggca agaaggcgtt gtacaccaca agggaggcgc cagcggagag gttggcgatc
      301 acgaagagaa atccaccaag caagtacgcg gaaccctgga ccaggtaaag ccccatggtc
      361 gaaaccgctc ccacgtatgc aaagagcatc aggagacgct tcttgcgtcc ggtcctgtcg
      421 gcgatagccc ccgtcaacgg caatagaaag acctgcaagc cgaccgagag cgagagcacg
      481 tagggaaaat atgacccggc caggatgggg ataccgaacg ggtacacgag gccgctggcg
      541 tcggccgcat tctctgtcac ggccgtcagg tatgggccga ggaagacggt gatcaccgtc
      601 gtaaagaacg cggagttcgc ccagtcatag aagtaccatc cccgctgctc gcggcggcga
      661 gacctctggt ctacgttccc gtcgtggttc atccgcgttg ccccagctcg aacacacgcc
      721 cgtgcaaggc ctcgacgacc tgctcccgcg gctccacgtt tccatcagga ccacgggggt
      781 cgaacggctc gccgaacgtg acgctgaccc gctccagcct gcgcaggagc tgtccccgcg
      841 gcatggcctc gtacgtacca cgaatgaaga cgggcacaac gggcaccggg tggtggtcca
      901 ggatcatgcc caccccgggc ttgaactgct gcaggcttcc cgtcctggag cgctcaccct
      961 cggcaaacca gacgaggttc tgaccgcgtg cgagcaccgc ggccccgtag gccaggctcg
     1021 agaacccggc gcgctcggga tcgacgggaa ctacctgtgc gaggcggctg atgagccggg
     1081 tgagcgggtt gccgaaggcg gcgccagtcc agcctgccca gtaggtgcgt cgccgcacgt
     1141 cgcggggcag tgccgctgcc accacgaaag catcgaggta gctgacgtgg ttcggcgcga
     1201 cgatgaacgg tccctcctcc ggcaggtttt cgagcccctc cgcccgcaag cggaacaagc
     1261 tcctcataat agcccggttg agtgagaaca tgcccccggc tagcgccgag gccaccctgc
     1321 caagcggtct cagccaccgc atttgcttct cgtccagaac ctcctcgggc cgttcgagcg
     1381 gcgaggcctg gtgcgccgtc ctgcccgtcc ccaaggcgac ctcgcttagt agatctcgga
     1441 gggtgtcgat gcgacctatg gctgcctccc cgatctctac accgatcctc tcgccgatct
     1501 ccagcgtaag gttgacccac ccgagcgagt ctatacccag gtcgagctgt gggctcgtgt
     1561 cgggggccaa tctcttttcg gggaatcgct cggcgagcag cgaccagacg ctcctcgcgg
     1621 ccgggttacg gagcagagcc tggtcgt
//
LOCUS       DBNGVD010000354         8612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2085402, whole genome shotgun sequence.
ACCESSION   DBNGVD010000354 DBNGVD010000000
VERSION     DBNGVD010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2085402"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16CM_09440"
     CDS             complement(<1..315)
                     /locus_tag="AC16CM_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3140093.1"
                     /translation="MSEGSGRQEMERRLVQRSIEDEAFRQRLIEDPKGAVEEDLGTRL
                     PEDVRVVVVEETQDTIYLVLPATPMAGDEGGELSDRELESVAGAGNATWVGYTECPCN
                     PIF"
     gene            934..1557
                     /locus_tag="AC16CM_09445"
     CDS             934..1557
                     /locus_tag="AC16CM_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140094.1"
                     /translation="MVGERPEAAYAARLLERQDALQTEANHLVESLDLPAVLGRAGRF
                     ERLGSSVSGLMAWRDLDLGVSCGVLGAERAWETMVPLAAHPRTTRISYRNEIGPLAPP
                     ELRGYGRYYFVARHQTEAGCEWKIDVSLWSPESPPGPLAHAEELMRRLTPETRLAILW
                     IKDAWHRLPSYPDVVSGMDIYEAVLEYGVRTPEQFGSYLSRRGLPTP"
     gene            complement(2051..2533)
                     /locus_tag="AC16CM_09450"
     CDS             complement(2051..2533)
                     /locus_tag="AC16CM_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140095.1"
                     /translation="MAAGGGAQRLAVRSCRPVRVHRLLRLGCHGTQNLRQAGAEAIRK
                     KAIAGSEGEQLKRVQDVLTVLNTPPLMVRLKVALEEDGVPYKEEELSVLQKIRRARND
                     LVHGRSREAPSEADLEYAVAVVNRMLLYRVTRLNTSAMESLLSEDPLHGDPFAVFDRL
                     "
     gene            complement(2872..3411)
                     /locus_tag="AC16CM_09455"
     CDS             complement(2872..3411)
                     /locus_tag="AC16CM_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140096.1"
                     /translation="MKFRDTEIAGDGVEGRVKNIVNDAAYAQVIIPNELLVNASPDYR
                     AAVEVSLVNGEESTALFTGFVDRVFTQEGKTRIELVTQTQFMKETGTGGLGLRRVDAR
                     EMMWALTILGGYAENEVEGWEPEPVEVFEIATAIDGILVDEPTTLGTVTLHSGGPVAT
                     LANGMGPDELREDYTCAPA"
     gene            complement(3431..3856)
                     /locus_tag="AC16CM_09460"
     CDS             complement(3431..3856)
                     /locus_tag="AC16CM_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140097.1"
                     /translation="MEEVTLEAGVEYTPLSSQRVTHIWEKPLREASGLVGQGWHEAAL
                     PQLLAAIRAVLAETLSVGIASLPSPLTPRSQGIKSLAPIAPHLPLLEACCKRIGEGLP
                     VDSDVAVPLAKELTPRVQQLAFNPPTTDDLKPLRESNSE"
     gene            complement(4971..5453)
                     /locus_tag="AC16CM_09465"
     CDS             complement(4971..5453)
                     /locus_tag="AC16CM_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3140098.1"
                     /translation="MFPTRILLATDGSKETPLAVEAAVRLANGTSSALHVVHVVSTVP
                     ELPYPRYWEKEKSEALLEWKKLRGLGFLDGLVWRIEEELGGNVAGSHYKEGAPDEEVV
                     RLGEEIGAGLIMVGGRRPKWLERVFLAGFPDKVLRRAKCPVLVVGERGSHGSPVAWRP
                     "
     gene            complement(5953..6339)
                     /gene="pcaC"
                     /locus_tag="AC16CM_09470"
     CDS             complement(5953..6339)
                     /gene="pcaC"
                     /locus_tag="AC16CM_09470"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HIR3140099.1"
                     /translation="MTSGDLGRERGMEVRREVLGDEHVDASVERTTPFTADFQDLITR
                     YAWGEIWTRPGLDRKTRSCITLTALMARGQLEELALHVRAALRNGLTEEEIKEVLLQG
                     AIYCGVPAANAAFAVAKRVLDDLSDR"
     gene            complement(6336..7127)
                     /gene="pcaD"
                     /locus_tag="AC16CM_09475"
     CDS             complement(6336..7127)
                     /gene="pcaD"
                     /locus_tag="AC16CM_09475"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HIR3140100.1"
                     /translation="MTRGRTIELHHLIEGPEDAPVLVLSNSLGTTLQMWRDQAPALSE
                     RFRLVRYDHRGHGGSPVPTGPYALDDLGRDVLALLDSIGAKRFSFCGLSVGGMVGMWL
                     ASEVPERVERLVLCCSAASFDPDAYSDRARTVREHGVSEVAGAVLERWFTPEFRATHP
                     DVVEWAGWMLIETPPEGYAGCCEAIAGADLAGRLGGVRAPTLVIAGAEDPAAPPGRAE
                     SIRDSVAGARLTILSPAAHLANVESPEAVTGAILEHLQADMREEA"
     gene            complement(7124..8479)
                     /gene="pcaB"
                     /locus_tag="AC16CM_09480"
     CDS             complement(7124..8479)
                     /gene="pcaB"
                     /locus_tag="AC16CM_09480"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HIR3140101.1"
                     /translation="MSEEIFGPIFVPEEFREAVSGRAWLQAMLDAEAALATAEAWVGL
                     IPHEAAAAIVSCCEATRFDPGELGHAGRGAGNPVPPLVKALTAAVSEVSRGAARHVHK
                     GATSQDILDTAAMLVARRAVDLVLAELTDICRACARLAEVHRGTVMAGRTLLQQALPT
                     TFGLKAAGWLVSVLEVSRRLSDLRDRGLAAQLGGASGTLASLGDSGVPVLGEFARELG
                     LAEPTLPWHTDRTRIGELGGSLSLAAGVLGKISLDVILMAQTEVGEVAEPAGEGRGGS
                     STLPHKRNPILSVIATANTRRVQAHAQTLYGSMAGEHERAAGAWHAEWEALSEALSLT
                     GGAAAAVREATEGLEVHPEKMRQNLEETGGILMAENVSTAVADRLGRLEAHEIVGAAA
                     RRSAEVGRPFREELLAEPALRKRLSSEEIDAALDPTYCLGSTGTFIDRALSLYRAEVQ
                     T"
BASE COUNT         1503 a         2847 c         2610 g         1652 t
ORIGIN      
        1 gaagatgggg ttgcacggac actcggtata gcctacccag gtggcattgc cagcgccggc
       61 aaccgactcg agctctcgat ccgaaagttc tccgccctcg tcgcctgcca tcggggtggc
      121 gggtagcacc aggtagatgg tgtcttgggt ctcctccacg accaccaccc gcacatcctc
      181 gggaagccgc gtccccagat cctcctccac ggccccctta gggtcctcta taagcctctg
      241 cctgaatgcc tcgtcctcga tgcttctctg gactagcctg cgctccatct cctgacgacc
      301 cgacccttcg ctcatgcgtg cctccccttt cgttctcggc ccaaccggct tgcgtgttcg
      361 ccctggtagt aaagcgcgaa ggggccagta tctcacgccc ccacctccgg gccatagagg
      421 ggacccgtac gcggcgaact actcagaaca ccgtagagcc gaagttcgca gaatactcca
      481 gcctgatgtg aaaataaggc cgccgcctgg aggagcctct tctatcaaga gtctgatgtt
      541 cgtgctctaa ggcaacaatt cggtgtaccc gaggctcctg cgtcacactg gtgtgtacgg
      601 gctttggctg caccaagcta agccgacgtc ggaccaaggc gacgacctct ccttgctttc
      661 atcccttcgg ggtggccctg caacaagggc catggatcac tgttgaaaaa ctccctcgcg
      721 ctcatttctg cccccggttc aggggacgaa tacgtcgttt gtgtaatatt ctggccttgt
      781 ttccggtctc tgtcggacca ccgaccgggc cgacaacgac tttttcaaca ggctggccga
      841 ttagcggaac ttcgcgttgc ctggcttcta cgggtgggat cccttcctgt gggaaaatgt
      901 tcggtcagga aagagtgaag gaggacagcc ccgatggttg gcgagcgtcc ggaggcggcc
      961 tacgccgcgc ggctgctgga gcgtcaggac gccctgcaga ccgaggcgaa ccatctcgtc
     1021 gaatcgcttg accttccggc cgtgctggga cgggccggac gcttcgagcg gctgggcagc
     1081 tcggtatcag gcctgatggc gtggcgggac ctcgacctcg gcgtttcctg cggggttctc
     1141 ggtgccgaga gggcctggga gactatggtg ccgctggccg cccacccgcg caccacgcgc
     1201 atttcctacc gtaatgagat cgggccactc gccccgcctg agctacgggg ctacggacgc
     1261 tactacttcg tggcccgcca ccagacggag gcgggctgtg agtggaagat cgacgtctcg
     1321 ctgtggtctc cggagtcgcc gccgggaccg ctcgcccacg cggaggaact aatgaggcgg
     1381 ctcacccccg agacgaggct ggcgatcctg tggatcaagg acgcgtggca ccggcttccc
     1441 tcctaccctg atgtggtgag cgggatggat atctacgagg cggtcctcga gtacggtgtg
     1501 cgcactcccg agcagttcgg aagctacctc agccggcgag gtctgcccac gccgtaggcg
     1561 agagcaccaa ccaaagcacc aacgacagca caccgtatgg ctgcagcgag gcccagcgga
     1621 agccgagaat tctccgaagt taggatgtcc gtcgaatatg cgccacggtg agaaattatg
     1681 cgatcagggt tgcaaccggt agcaaccgta cgtagcatga gtatctaatg cggttggttg
     1741 gcagttctcg tcgatggtcg agcgcctttc gaggagtcgt ccgattcttc gaccgtgctc
     1801 aggaagccga tttctatgca agatcgtgaa ttgcggacaa tgccccttct gcgaaattcg
     1861 gattcttcgt atgtgtcaga gccgttgcct agggaaagac gttagcggct cctggccagt
     1921 gttgttgcaa gcgtaaaact agtatctact tgtaccgacc tgtaccaacc ctctatcgca
     1981 ggcgtcagac gtccgaggtg aggctgcgag tcgacccaag atatgtgcag tgcgtccctt
     2041 atgcttcatc ttatagacgg tcgaacacag cgaaaggatc cccatgcagc ggatcttcgc
     2101 tcaggagcga ttccatcgcc gaggtgttta gccgtgtgac tctgtaaagg agcatgcggt
     2161 tgaccaccgc caccgcatat tctaggtccg cctccgaagg cgcctcgcgg gagcggccgt
     2221 gaacgaggtc gttgcgcgcc cgcctgatct tttggagcac cgatagctct tcctccttgt
     2281 agggaacccc gtcctcctcc agcgccactt tcaggcgcac catgaggggt ggcgtgttaa
     2341 gcacagtgag cacgtcctgg acacgcttga gctgctcccc ttccgacccc gcgatggctt
     2401 tcttacggat ggcttcagca ccggcttgtc gaagattttg cgtgccgtgg cacccgaggc
     2461 gtagaagtct atggactctc acagggcgac aactgcggac agcgagtctc tgtgctccgc
     2521 cgcccgccgc catgcggaga tcgcctcgct cacctgaacg ggcagttgga cagggagcat
     2581 cggaatggtc aggtcttcca tttattcaag ggcgagttgc gggcgcgcta gtatgccttc
     2641 aatgccgcgc agccactgcc tgcccttcgc tgtcccccgc actacgacga catttcgacg
     2701 cgacacgcgt gaagacgtcc actcccggcg aaatccgcga gggggccttc caggcaggtt
     2761 cacgctgctg tactgggatt tggccgtcag ccaagccagg gccaagtcga tgtctctgat
     2821 gcctgccgct tcagcctcgt ggagggtgcg ggctgtcttg agcgtgagtg ctcacgcggg
     2881 cgcacaggtg tagtcttctc ggagttcatc aggccccatt ccattggcca aagttgccac
     2941 tggaccaccc gaatgtagtg tgaccgttcc gagcgtcgtg ggctcgtcca ccagaatgcc
     3001 gtcgattgcc gtcgcgatct caaagacctc gacgggttcc ggctcccacc cctccacctc
     3061 gttctccgcg tagcccccca gtatcgtgag tgcccacatc atctcacggg cgtcgactcg
     3121 cctgagaccg agaccacccg taccggtctc tttcatgaac tgcgtctggg taacaagctc
     3181 tattcgtgtc ttgccttcct gggtgaacac acgatcgaca aagccggtga acagagcggt
     3241 gctctcttca ccattgacca aggagacctc cacggcagcc cggtagtccg gactggcgtt
     3301 cacgagaagt tcgttgggaa tgattacctg agcataggcc gcgtcgttca cgatgttctt
     3361 caccctgccc tcgacgccgt cacctgcgat ctctgtgtcc ctgaatttca caacaatgcc
     3421 gtctcggggt ctactcacta ttagattccc tcagcggctt gagatcgtcg gtggttggtg
     3481 gattgaaggc aagctgctgg acccgaggag tcaactcttt agcaaggggc acggcgacgt
     3541 ccgagtctac gggcaaccct tcacctattc gcttgcagca cgcctcgagc aagggcaggt
     3601 gcggcgcgat cggagcaagc gacttgattc cctgcgatcg gggcgtgagt ggtgagggaa
     3661 ggctcgcgat accgacggac agcgtttcgg ccagcactgc tcggatcgcc gccagcagct
     3721 gcggcagggc ggcctcgtgc caaccttgtc cgaccaggcc cgaagcctcg cggaggggct
     3781 tctcccaaat gtgcgtgact ctctggctgc ttagaggcgt gtactcgacc cccgcttcaa
     3841 gagtcacttc ctccaagcgt gcctcacttt ctctcagcaa ccctgccagc gtagcggccc
     3901 ggcgccttat gagaacgaag gcaccggcct cccaccacga ctcgacggac cggcccggcc
     3961 gaatgatcac gcggcgtcca gtaggttcct ccaaaccttt gagggcggcg atgaaatcaa
     4021 tctgggcaca catggaggca atctgaagtc ggtagaatct ctccgcggca tcatgggggc
     4081 tttgctcagc cgcaaaagtc cctggtcgct caagggaaac tcttcgatta caatctcatc
     4141 tagcccgcaa cgccgccaga gctcaatgaa gcgctcggtc cacgccttgt gggtagtcaa
     4201 gaaagattgc cacattcccc tcgcttcggc agtagcccgc tttgcatttt cacttgccaa
     4261 cgcctctgac caggcctttt caagtccgac ggtcatcttg gtcaggtgct cgagcaagtc
     4321 ggcgggcgaa gtcgcccccg atccgacctc cccgcccgag gacgccgttt cctcactcat
     4381 cctcgccccc acgccgacct aaactaccaa atcgcgacca gcggtagatc ctagttcggt
     4441 gccatagcta gaggtgctcc acatgagcgc aggtcatacc cttccggatc ttaatttagc
     4501 gctggatgta cctttgcgag aggtgtgcgg accacatgtg tcaattgtcc cgaaaacgca
     4561 tgacatatca agagctggga ttgttcaatt gagacggtgt ttagagactt gatcttggcc
     4621 tttcaggaca cctttttggg acgaatcctc tgcgtcccga taacgagagt ttcggggatt
     4681 caatcccgag cattgctata cgaagggtcc ttcccatccc ctaccgaccc gtaccatcac
     4741 gttcgcggta ccgttgcagt acgccgaagg agctgtcttt cacttccctg cttgccgtga
     4801 gttgcaccgc gttgcgctcg cggtggtgtc agagtcttgt ccggagtaca accttcgtat
     4861 ccggtagcgc catcgtgatg ccccctccag cttgcgccgc acccgctcct tggggctagg
     4921 atgcgagtag aaagacttga gcttcggtgt gtcaccgtcg ggggacgagg ctagggccgc
     4981 caagctacgg gagatccgtg cgaaccccgt tcacccacca ccagcaccgg gcacttcgcc
     5041 cgtcgaagga ccttatcggg gaagcccgcc aagaagaccc gctcgagcca ctttggcctc
     5101 cggcctccca ccatgatcag accggcgcca atctcttcgc cgagacgcac cacctcctcg
     5161 tcgggggcac cctccttgta gtgagaaccc gcgacattcc ccccgagttc ctcctctatg
     5221 cgccacacca aaccgtctag aaacccgagg cccctgagct tcttccactc cagcaaagcc
     5281 tcgctctttt ccttctccca gtaacgcgga tacggcaact cgggcacagt ggaaaccacg
     5341 tgcaccacgt gcaacgccga gcttgtgccg tttgccaagc ggaccgcagc ctcgacggcc
     5401 agaggcgtct ccttggagcc gtccgtcgcc agcagtatcc tcgtcgggaa tatggtgttc
     5461 tccttattct agatgccaac tgcacataaa tctcggttag cgtatgccga ccgccaagag
     5521 atgtttgtga atccgatcgc caacgagtgg ataggtttcg gtccggaggg cgacttccgc
     5581 ggtcatctcg gcccacgctc gatgcaagaa ccgctttgaa ggagagaggg ataccagcac
     5641 gcgtttgcag agctggctct tacacggact acccggcggc ccccggcact cgctggcctt
     5701 gcctgccggt ggcagcttcc acagaggtgc tcacctccga cggcgactgc ggctaccgcg
     5761 cgggagattc gcctggagga ggtgctcagc cacctcgggc ggcaggtact tgtcgcattc
     5821 ttcaaggctt cacaaaaggt gcgagagtag cgccccctcc ggcccgtctc ctactatccc
     5881 tgcccgtgtg cgcccgctcg ttcccgctcg ggtggtggtt gcggcgtcga tgggctccgc
     5941 tgtgccgaga cgctaacgat cgctgagatc gtccaagacc cgcttggcga cggcgaaggc
     6001 ggcgttggct gccggtacgc cgcagtagat ggctccctgc aggaggacct ccttgatctc
     6061 ttcctcggtg agaccgttgc gcagggccgc cctcacgtgc agcgccagct cctccagctg
     6121 tcccctggcc atgagcgcgg tcagggtgat gcagctacgg gtcttgcggt cgagcccggg
     6181 tcgcgtccag atctcgcccc acgcgtagcg ggttatgagg tcctggaagt cggcggtgaa
     6241 cggcgtcgtt cgttccaccg aggcatcgac gtgctcatcg ccaaggacct ccctgcgtac
     6301 ctccatgccg cgctcgcggc cgaggtcgcc actagtcatg cctcctccct catgtcggct
     6361 tgcaggtgct cgagtatcgc ccccgttacc gcttcggggc tttcgacgtt ggccaggtgc
     6421 gccgccggcg agaggatcgt aaggcgcgcg ccggccaccg agtcacggat cgactccgcc
     6481 cgacccggcg gcgcggcggg atcctccgcc cctgcgatga cgagcgtggg ggcgcgcacg
     6541 ccgcccagcc tccctgccaa atcggcgccg gcgatcgcct cgcaacagcc cgcgtatccc
     6601 tcgggcgggg tctcgatcag catccacccg gcccactcaa cgacgtccgg gtgggtcgcg
     6661 cgaaactcgg gcgtgaacca gcgctcgagg acggccccgg ctacctccga tacgccgtgc
     6721 tcgcggaccg tcctggcgcg gtcgctatag gcgtcgggat cgaagctcgc ggccgagcag
     6781 cacagcacca gtcgctccac gcgctccggg acctcgctgg cgagccacat gccgaccatc
     6841 cctccgacgg agaggccgca gaaggagaag cgtttcgccc cgatgctgtc caggagcgcc
     6901 aggacgtctc ggccgaggtc gtccaaggcg taggggccag tcgggacggg ggaacctccg
     6961 tggccgcggt ggtcgtagcg cacgaggcgg aaacgctcgc tcagtgcggg cgcctggtcc
     7021 cgccacatct gaagcgtcgt gcccagggag ttcgagagca ccaggaccgg cgcatcctcc
     7081 ggaccctcga tcaggtggtg tagctcgatg gttctgcccc gcgtcacgtt tgcacctccg
     7141 cgcgatacag agacaacgct cggtcgatga atgttcccgt agaaccgagg cagtacgtgg
     7201 gatccaacgc cgcgtcgatc tcttcggaag agagacgttt gcgcagtgcc ggctccgcca
     7261 ggagttcctc acggaaaggc ctgccaactt ctgcggacct cctggcggcc gcccccacga
     7321 tctcgtgggc ctccagtcgt ccgagtcggt cggctacggc cgtgctaacg ttttcggcca
     7381 tgagtatccc ccccgtctcc tcaaggttct gccgcatctt ttccggatgg acctccaacc
     7441 cctccgtcgc ctcccgcacg gctgccgcgg cgccgcccgt cagggagaga gcttcggaga
     7501 gcgcctccca ctcggcgtgc caggcgccgg cggcgcgctc gtgctcgcca gccatggacc
     7561 cgtagagcgt ctgcgcgtgc gcctgaacgc ggcgggtgtt cgcggtagct attacggaga
     7621 ggatggggtt tcgtttgtgg gggagcgtcg aggagccacc acggccctcg ccggcaggtt
     7681 cggcgacctc ccccacctcg gtctgggcca tcaggataac gtccagggag atcttcccga
     7741 gcaccccggc ggcgagcgag agggagcccc cgagctcccc gatcctcgtc cggtcggtgt
     7801 gccagggcag cgtgggctcg gcgagcccca gctcccgcgc gaactccccg agtacgggga
     7861 cgccggagtc gccgagtgag gcgagcgtcc ctgaggcccc accgagctgg gccgcgagcc
     7921 cgcggtcccg caggtccgat agccttcggc tcacctccag aacggacacg agccaaccgg
     7981 ctgccttcag gccgaaggtg gtggggagtg cctgctggag cagggtccgc ccggccatga
     8041 cagtcccgcg atgcacttcg gccagccggg cgcaggcccg gcagatgtcc gtgagctcgg
     8101 ccaggaccag atctacggca cgccgggcga cgagcatcgc ggccgtatcc aggatgtcct
     8161 ggctcgtggc gcccttgtgg acgtggcggg cggcccctct tgagacctcg gagacggctg
     8221 cggtcagggc ctttacgagc ggcgggacgg ggttaccagc cccccgcccg gcgtgtccaa
     8281 gctctccggg atcgaagcgc gtggcctcgc agcacgagac gatggccgct gccgcttcgt
     8341 gggggatcag gcccacccac gcctcggccg tcgcgagggc ggcctcggcg tccagcatcg
     8401 cctgtagcca ggcccgtccg gagactgcct cgcggaactc ttcggggacg aagatgggtc
     8461 cgaagatctc ctccgacatg cctaaagctc gaagaagacg gtctggtcgt cgccctgcag
     8521 gcgtacgtcg aaacgcaaag cgcctccttc gtcgcgggcc aggagcgtct ccctggaccg
     8581 tggatcctcg atagacgaga gtagcgggtc ac
//
LOCUS       DBNGVD010000355         3746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1722081, whole genome shotgun sequence.
ACCESSION   DBNGVD010000355 DBNGVD010000000
VERSION     DBNGVD010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1722081"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="trpD"
                     /locus_tag="AC16CM_09485"
     CDS             complement(<1..726)
                     /gene="trpD"
                     /locus_tag="AC16CM_09485"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3140102.1"
                     /translation="MLRDTLRKAASGESLTEGEAERALETIMEGNVPPAATAALLTAL
                     RVKGESVPEIVGFARAMRRFAGKVDAPESVVDTCGTGGDAKGTINVSTAAAFVARGAG
                     VVVAKHGNRAATSRAGSADVLEALGAEIELGPAAVSRCIDESGIGFMFARTHHPAMRF
                     VAPVRAELPFRTVFNLLGPLTNPAGARRQLVGVFGGAYVRPVAEALRDLGARKALVVH
                     GKDGMDEITVTDATIVADVSEEGI"
     gene            complement(731..1294)
                     /locus_tag="AC16CM_09490"
     CDS             complement(731..1294)
                     /locus_tag="AC16CM_09490"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3140103.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVLVRRNDEVTPDEVSGLH
                     PDRIVVSPGPCTPNEAGVSVDLIGRVGEHTPTLGVCLGHQAIGQAFGAKVVRGEPVHG
                     KTAKIRHDGEGVYRGLDQDFEATRYHSLVIEPESVPECLIVTSRTEDGTIMGVRHREY
                     PVEGVQFHPESVLTRSGHDLLKNFLET"
     gene            1346..2296
                     /locus_tag="AC16CM_09495"
     CDS             1346..2296
                     /locus_tag="AC16CM_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3140104.1"
                     /translation="MTLLLSENEVEELLDMPSTLEAVESVLRQHAEGRATNRARRRVA
                     LPTGGLNFMAAGAPEIGALGLKAYTVARSGARFYTLLFDPDGGELLSIMQSDKLGQVR
                     TGAASGVATKHLAREDANTLGIYGAGWQAESQLEAIAAVRDLERVIVYTRSEETRKSF
                     AERMGERLGMEIETTHAAEEPAAQDIVVTVTSSREPVLLGEWLRPGAHVNAAGSNFLF
                     KAEIDRQVVGRASFVCADSREELGLEAGDLMLSLETGAILPEAIYELGQVVAGQVQGR
                     RGPEDITLFASQGLALEDMAAARVVYDRAVERDVGRDIDF"
     gene            complement(2323..3432)
                     /locus_tag="AC16CM_09500"
     CDS             complement(2323..3432)
                     /locus_tag="AC16CM_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3140105.1"
                     /translation="MGVNDEKGKIRRRATLKDVAAEVGVSPATVSNAYNRPEQLSGAL
                     RVKIMEAARRLGYAGPDPTARGLRRGRVGAIGVLYADRLSYAFADPAAVLFFEGVSRV
                     AEEAGFALLLVPGSISGRHDPKAVRGAAVDGFVVYCMAEDDRMIGAVRERRLPAVFVD
                     DPPKASSGDLPIVRVDDEGGARAAAEHLTGLGHRRVAVVTFELAPSPEGGLADLERQA
                     QTSFRPTRSRLDGYKKGLGNAGVRWEDVPVYECPENVPAEGARAARFLLSMDPRPTAI
                     LAASDQLAFGVMEAAKDMGLSVPGDFSVVGFDDVPEAARSDPQLTTVHQDHVAKGLLA
                     GRMLVARLEGEEPEAPAVLPTHLVQRASTAPPTPL"
     gene            3493..>3746
                     /locus_tag="AC16CM_09505"
     CDS             3493..>3746
                     /locus_tag="AC16CM_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140106.1"
                     /translation="MVGLMNVREQVDLDYARARRKAFLTGLWARVRRRCASLCAFDAV
                     RGELGARNRRYVGARTVEVSRVVGSVGRWKEFDSGFMPRRA"
BASE COUNT          626 a         1234 c         1274 g          612 t
ORIGIN      
        1 tatcccctcc tcagacacgt ccgcgacgat ggtcgcatcg gtaaccgtga tctcgtccat
       61 accgtccttg ccatgaacga ccagggcttt tcgggcaccg agatccctga gcgcctcggc
      121 gacgggcctc acgtaggccc cgccgaagac cccgacgagc tggcgcctgg ccccagccgg
      181 gttggtgagc gggccaagca ggttgaagac ggtcctgaac ggtagctccg cccgcacggg
      241 cgccacgaac cgcatggcag gatggtgcgt gcgggcgaac atgaagccta tccccgactc
      301 gtcgatacag cgggagaccg ccgccgggcc cagctctatc tcggcgccca gtgcctcaag
      361 cacgtcggcg ctccctgccc tgctggtggc cgcgcgatta ccgtgcttgg cgacgactac
      421 cccggccccg cgcgccacga aggcggctgc ggtggagacg tttatggtgc ccttggcgtc
      481 cccgcccgtg ccacaggtgt caaccacgct ctcgggcgcg tcgaccttcc cggcgaagcg
      541 tcgcatggcg cgggcgaacc cgacgatctc gggcaccgac tcacccttga cgcgcagcgc
      601 ggtcaggagc gcggcggtgg cggcgggcgg aacgttgccc tccatgatgg tctcgagcgc
      661 gcgctcggcc tcaccctcgg tcagcgattc gccgcttgcg gctttcctca gcgtatcgcg
      721 cagcaaaggc tcaggtctcc aggaaattct tcaggaggtc gtggccgctg cgggtcagaa
      781 cgctctctgg gtggaactgg acgccctcga ccgggtactc gcggtgcctg acgcccatga
      841 tcgtgccgtc ctcggtcctg ctggttacga tgaggcactc cggtacagac tcgggctcga
      901 tcacgagcga gtggtaacgg gtggcctcga agtcctgatc cagccccctg tagacccctt
      961 ctccgtcatg ccttatcttc gcggtcttgc cgtggacagg ctcgccgcgc acgactttcg
     1021 cgccgaaggc ctggcctatg gcctggtggc cgaggcagac gcccagggtg ggtgtgtgct
     1081 cgccgacccg tccgatgagg tcgaccgaca ccccggcctc gtttggcgtg caaggaccgg
     1141 gcgagactac tatcctgtcc gggtggagcc cggagacctc atcgggcgtg acctcgtcgt
     1201 tgcgccgcac gagcacctcg gccccgagtt ccccgaggta ctggaccagg ttgtacgtaa
     1261 aggagtcgta gttgtctatg acgaggaccc tcatgagcag agcgatttta acggataagc
     1321 cgccggcgaa cagggaggag atatcgtgac gcttctgctg tccgagaacg aagtggaaga
     1381 gttgctcgac atgccttcga cccttgaggc cgtcgagtcc gtgctgcgcc agcacgcgga
     1441 gggacgggcc accaaccggg ctcgcaggcg cgtggccctc cccacgggcg ggctcaactt
     1501 catggcagcc ggggcgcctg agataggggc cctcggcctg aaggcctaca cggtcgcccg
     1561 aagcggcgcc cgcttctaca ccctgctctt tgacccggat ggtggggagc tgctctcgat
     1621 catgcaatcg gacaaactcg ggcaggtgcg caccggcgcc gcgagcgggg tggcgacgaa
     1681 gcacctcgcc agggaggacg cgaacactct agggatctac ggggcaggct ggcaggcgga
     1741 gagccagctc gaggccatcg ccgccgtccg ggacctggag agggtgatcg tctacacccg
     1801 cagcgaggag acgcgcaagt cgttcgccga acggatgggc gagcgtcttg ggatggagat
     1861 agagaccacc cacgccgcag aggaaccggc agcccaggac atcgtcgtca ccgtgacctc
     1921 atctcgggaa cccgtgctcc tcggggagtg gctgaggccg ggggcgcacg tcaatgcggc
     1981 cggctccaac ttcctgttca aggccgagat cgaccgccag gtcgtcgggc gggcaagctt
     2041 cgtgtgcgcc gactcacgtg aggagctggg cctggaggct ggcgacctga tgctgtccct
     2101 cgagaccggc gccatcctgc ccgaggccat ctacgagctt ggccaggtcg tcgccggcca
     2161 ggtgcaggga cgccgcggac ccgaagatat aaccctgttc gccagccagg gcctcgccct
     2221 ggaggatatg gccgcggccc gcgtcgtcta cgatcgcgcc gtcgaacgcg acgtcggcag
     2281 ggatatagat ttctgaggta gggccgggtt tcaaccccgc cactaaaggg gtgtgggtgg
     2341 cgcggtagag gcgcgctgga ccaggtgggt cggcaagacg gccggggctt caggttcttc
     2401 gccttcgagc ctggcgacta gcattcgtcc cgcgagtagg cccttcgcca cgtgatcctg
     2461 gtgcacggtg gtgagttgcg ggtcggagcg tgcggcctct gggacgtcgt cgaacccgac
     2521 cacggagaag tctcctggta cggacagacc catatccttg gccgcctcca taacaccgaa
     2581 ggctagctgg tcgctggcgg ccaggatcgc cgtcgggcgt gggtccatgg agagcaagaa
     2641 cctggcggcc ctggctcctt ccgcaggaac gttttcgggg cactcgtaga cggggacatc
     2701 ctcccagcga acaccagcgt tcccgagacc cttcttgtag ccgtcgaggc gagaacgtgt
     2761 cggcctgaag gaggtctggg cctgacgttc gaggtcggcc aggccaccct ccgggctcgg
     2821 ggcgagctcg aaggtcacga ccgccacgcg ccggtggccc agcccggtga ggtgctcagc
     2881 ggccgcgcgg gcaccgccct catcgtccac gcgcactatg ggtagatccc cggacgacgc
     2941 tttcggggga tcgtcaacga acacggccgg caacctccgc tcgcgaaccg ccccgatcat
     3001 gcggtcgtcc tcggccatgc agtagacgac gaaaccgtcc accgcggccc ccctgacagc
     3061 cttggggtca tgcctcccag agatcgagcc aggcacgagc aagagcgcga agcccgcctc
     3121 ctcagccacc ctggagaccc cctcgaagaa gagcacggcc gccggatcgg cgaaggcgta
     3181 ggagagcctg tccgcgtaga gcaccccgat ggccccgacc ctcccccgcc tcaacccccg
     3241 cgccgtgggg tccggcccag cataaccaag ccgccgagcc gcctccatta tcttgacgcg
     3301 taaggcgccg gagagctgct ccggcctgtt gtacgcgttg gagacggtcg ccggcgatac
     3361 cccgacctcg gccgccacgt ccttcaacgt ggcccgacgc ctgatcttcc ccttctcgtc
     3421 gttgacaccc atctggcata tgatactatg actgaaacga ttaagtaaaa cgatttagaa
     3481 aggcggtgtg gcgtggtcgg attgatgaac gtacgggagc aggtagatct ggactacgcc
     3541 agggccagga ggaaggcgtt cttgactggg ctctgggcgc gggtacggcg gcgttgtgct
     3601 tctctgtgcg ccttcgacgc ggtgcgggga gagcttgggg cgagaaaccg gcgttacgtc
     3661 ggggcgagga cggtcgaggt aagccgggtg gtcggtagcg tcggccggtg gaaggagttc
     3721 gacagcgggt tcatgccgag gcgggc
//
LOCUS       DBNGVD010000356         6532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4091027, whole genome shotgun sequence.
ACCESSION   DBNGVD010000356 DBNGVD010000000
VERSION     DBNGVD010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4091027"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16CM_09510"
     CDS             <1..357
                     /locus_tag="AC16CM_09510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140107.1"
                     /translation="VVGKRAIPKVGAYCATKSALDALSDALRVEMGARITVTSVFPGT
                     TRTNFRENSRRTKSEKRGWRPKGVTPDRVAVRIADAAEDGPRDVYVTLPDRLFVAGVT
                     LFPGLADRVLRSWAKD"
     gene            362..988
                     /locus_tag="AC16CM_09515"
     CDS             362..988
                     /locus_tag="AC16CM_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140108.1"
                     /translation="MGFEESALERLRRYRGEADRSIGAVHEAEAVARRFADRLFAGLE
                     YTAVLGRQAGFEVQTTYAGGALDLRTVAGGGAEAGVSFGVVPGAAAETDEDLMHEEFS
                     HYSLDPSGYSGRILGWSEEAPDIPCQTFAVYRDGVWKTTGLFVARARGNVEDPDDVVN
                     GFCLRILGRLIDVAALTGGAGRRWTEEPYSLQELLEARRPPTALRWPK"
     gene            1215..1880
                     /locus_tag="AC16CM_09520"
     CDS             1215..1880
                     /locus_tag="AC16CM_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140109.1"
                     /translation="MWWQSLAILARHPVATVAPALILGPLSEAPHLLSSDRSYFEEVV
                     VFTVESLVFYLYVAYAERLTSEARSNSRPIPALRVLRNLALAAPVVPIVAFSSLAAIA
                     LPTAAASLLVIPGIWLMTRWSLFAPAIVSEHLGPVASLKRSNELVKGNFELVFLTAAL
                     AVVLEEAFESAGTLTGLVLTGSETWGPWLGGSVGTIIILPAASFATALVYGYLRRAED
                     RAR"
     gene            complement(1947..2882)
                     /gene="coaA"
                     /locus_tag="AC16CM_09525"
     CDS             complement(1947..2882)
                     /gene="coaA"
                     /locus_tag="AC16CM_09525"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIR3140110.1"
                     /translation="MDLSPFLRFSREEWSRLRATAPLTLSEEDLVALQGLNESVSMEE
                     VVEIYLPLARLLNLHLGASRALYNVTDSFLGNPAAKVPYVIGIAGSVAVGKSTTARIL
                     QSLLSRGPKRPGVSLITTDGFLYPNRILEERGLMKRKGFPESYDLKRLMRFMADVKAG
                     YPEVSAPVYSHLTYDILPEEKEYVRQPEVLIVEGLNVLQAGNGHPRNAPPLFVSDFFD
                     FSIYVDADEAQTEKWFSERFLTLRDTVFKDESSYFHRYAHLSTEEAKEVAAGIWAEIN
                     GVNLRENIAPTRERAHLILQKGEDHQVLGVRLRKL"
     gene            complement(2908..3207)
                     /locus_tag="AC16CM_09530"
     CDS             complement(2908..3207)
                     /locus_tag="AC16CM_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140111.1"
                     /translation="MILLRDARGGRPDNAAGIGEFWYEPEVWTLPLSPDARVLYAGLC
                     SFLGHREINRHDLRNTLKGRPDDEIAHALEELIHHDLIVPDASRGYEVRSVGEFG"
     gene            complement(3243..5678)
                     /locus_tag="AC16CM_09535"
     CDS             complement(3243..5678)
                     /locus_tag="AC16CM_09535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3140112.1"
                     /translation="MSDPIPKDAGTEAGTPEELADEPLLDRLPPEDLPEELLVRRLGR
                     LDTRRVLAELCRNVPGLEEVLLPRGIQADALTRKSGGAPLLKGLARRISHDAAAWGAF
                     RTAIQEQIPPETSAALEDLTLENLEEARDAHTTEGLLLAALASEEEQKDGVVGALVDA
                     WREEKGEAEKKASKDAHVRDLEAELERLKGENERLSFASRAAKERAAALAEEVDVLTG
                     ERTDTSELVKKAEEKAAAALEVQGQLEERIETLEKRGFQVERALEGERRAYSRAAERV
                     DELHEELGGVVEERDRIKNALQNARFTDRGFGELLIRAIENEVAALPTSVESTAQAAR
                     LMEFMGKVLQAHTELGGPGKSREEDPDDMEPSPAGPTKETGRSVAVGGYARPRPTLSF
                     QALGGAGEVGGSSHLLDFGKSKILVDAGIKPDGRGPATPDFSKLNGVDAAVVTHAHLD
                     HCGALPLLVRDRPNVPIYCTPPSAKLIVSALNDHAAMGGGLPGGAPIHEVRKRLVPVP
                     FGKPLDIGGTKVTLTESGHILGAASVLFNSGPSTVFHTGDICMEDHFSIPSARLPDVR
                     DIDLLIMEATLADQKPQPFAESIRTMVEVINDTTLERGGTVLIPTYALGQAQEIILGI
                     KHYAKEYGLDKEVFIYVDGSVVTTSERLYAEQLGYMKPYLQHSDPREIFFSDNIRAVA
                     NDDKARERILSNPCAIIASPVTMQGGASAFYRKRLEKHAQNAVILPSNAAASYGTHRA
                     EGEEEQWRVEHVSFAAHCTQDELLGITEKLSPRQIILIHGSRRRISDLAFRLAPDHKI
                     HTPAVGETVRTVL"
     gene            5889..6161
                     /locus_tag="AC16CM_09540"
     CDS             5889..6161
                     /locus_tag="AC16CM_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140113.1"
                     /translation="MEGTPAQDNVRILAHSPGRYPILVIELPSGELRTAYLETGYDLR
                     HTKPVVRGWLQDNAIGRHSFVALDPPVEWPVDSLADYTRNELLQKP"
     gene            complement(6241..>6532)
                     /locus_tag="AC16CM_09545"
     CDS             complement(6241..>6532)
                     /locus_tag="AC16CM_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140114.1"
                     /translation="SGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGG
                     GSGGGDGSGGDGSGGGGSGGGGAGGDGSGGGGSGGGSRTQDSRGDGTGGGRR"
BASE COUNT         1017 a         2197 c         2138 g         1180 t
ORIGIN      
        1 gtggttggaa agcgcgccat tccgaaggtc ggcgcctact gcgcgaccaa atctgccctg
       61 gacgcgctct ccgacgccct cagggtggag atgggcgcga ggatcaccgt caccagcgtc
      121 tttcctggca cgacccgcac caactttcgg gagaattcgc ggcgcacgaa gagcgagaaa
      181 cgcggctggc gtccgaaggg cgtaaccccg gacagggtcg cggtgaggat agccgacgcc
      241 gccgaggatg gcccccgcga tgtgtacgtg acgcttccgg accggctctt cgtcgccggc
      301 gtcacgctct ttcccggtct cgccgaccgc gtgctgcgtt cctgggcgaa ggactaggcg
      361 ggtgggtttc gaggagagcg ccctcgagcg cctgcgccgc taccgcggcg aggccgaccg
      421 ctctatcggg gccgttcacg aggccgaggc ggtggcccgc cggttcgccg accgtctctt
      481 cgccgggctg gagtacacgg cggtgctcgg caggcaggcc gggttcgagg tacagaccac
      541 ctacgccggc ggcgcgctcg acctgcggac cgtagcggga gggggtgcag aggccggggt
      601 ctccttcggg gtcgtgcccg gtgccgccgc cgagacagac gaggacctca tgcatgagga
      661 gtttagccac tacagcctcg atccgtcggg ctattcagga cgcatcctgg gctggtccga
      721 ggaggccccc gacatcccct gccagacctt cgccgtctac agggacggcg tatggaagac
      781 caccggcctc ttcgtggccc gtgctcgcgg caacgtagaa gaccccgacg acgtcgtaaa
      841 cggattctgc ctcaggatcc tggggcgcct catcgacgtc gccgccctta ccggcggcgc
      901 gggcagacgc tggacagaag aaccatactc gctgcaggag cttctggagg cccggcggcc
      961 ccccacggcc ctacgttggc cgaagtgagc cgtaggctat cagcaagagc cccggggtgc
     1021 tgggaaaagg atgaccctgg tctctgggga tggcatcatg ccccggacgc aggtcggttc
     1081 gaccctcttg caatcagcat ggttgacagg aaactttcgg ccctcgtatc cgctggccgg
     1141 gagctgaagg ctgactgctg atagctgata gctaaccagg aggcggtctg aaagcgcggg
     1201 tccgtacatc ggtggtgtgg tggcagtcgc tcgcgatcct ggcgcgccat cccgttgcga
     1261 cggtcgcgcc ggctttgatc ctgggaccgc tctccgaggc gccacacctg ctatccagcg
     1321 accggtctta tttcgaggag gtcgtggtgt tcactgtgga gtcgctcgtt ttctacctct
     1381 acgtggcgta cgcggagcgg ctgacctcgg aggcgcgaag caactccagg cccatcccgg
     1441 cgctcagggt gctcaggaac ctcgcccttg ctgcccccgt ggtaccgata gtcgccttct
     1501 cctccctggc ggcgatagcc cttccgaccg cggcggcgag cttgcttgtg atacctggca
     1561 tctggttgat gacccgctgg tcgttgttcg ccccggcgat agtcagcgag cacctcgggc
     1621 ctgtggcgtc cctgaagcgc agcaacgagc tggtaaaggg caacttcgag ctggtcttcc
     1681 ttaccgccgc ccttgccgtg gtgctcgagg aggccttcga gtccgcggga acgctgaccg
     1741 gcctggtgct gaccggctcg gagacgtggg gaccatggct gggtggctcg gtcggcacca
     1801 tcataatatt gccggcggcg tccttcgcga ccgcgctcgt gtacggttac ctgcggcgcg
     1861 cggaagaccg cgcccgctag tgagcccgag tagaccgtgt ccccgtcggg cggttcgcga
     1921 accgcccaac cacgtaccgc cgcgccctac aatttccgca ggcgcacccc taggacctgg
     1981 tggtcttcgc ccttctgtag gatgaggtgc gcgcgttccc tggtgggggc gatgttctcc
     2041 ctcaggttca ccccgttgat ctcggcccag atcccggcgg ccacctcttt ggcttcttcc
     2101 gtgctgaggt gggcgtagcg gtggaagtat gaggactcgt ccttgaacac cgtgtccctg
     2161 agcgtcagga accgctccga gaaccatttc tcggtctgcg cctcgtcggc gtcgacgtag
     2221 atggagaagt cgaagaagtc ggagacgaag agggggggcg cgtttctggg gtggccgttg
     2281 ccggcttgaa gcacgttgag gccctccacg atcagcacct caggctgcct gacgtactcc
     2341 ttctcctcgg ggagaatgtc gtaggtgagg tgggagtaga cgggggcaga aacctccggg
     2401 tagcccgcct tgacgtcggc cataaagcgc atcaggcgtt tgaggtcgta gctctcgggg
     2461 aagcccttcc gcttcatcag gccacgctct tcgaggatgc ggttcgggta cagaaagccg
     2521 tcggtggtga tgagggacac ccccgggcgt ttcgggcccc gggagagcag gctctgcaga
     2581 atccgcgccg tggtgctctt gccgacggcg acgctacccg cgatgccgat cacgtaggga
     2641 accttcgccg ccgggtttcc gagaaaggag tccgtaacgt tgtagagggc ccgcgaggcg
     2701 ccgaggtgga ggttcaacag ccgtgcgagg ggaaggtaga tctcgaccac ctcttccatc
     2761 gaaacgctct cgttcaggcc ctgcagggcc acgaggtcct cttcgctcag cgttagcggt
     2821 gcggtggccc tcagcctgct ccactcctca cgcgagaacc ggaggaacgg cgagaggtcc
     2881 atgacgttac cttcgttcgt cacgccacta cccgaactct cccacagaac gaacctcgta
     2941 tccccgggaa gcatcgggga cgatcaggtc gtggtggatc agctcctcga gggcatgggc
     3001 tatctcgtcg tcggggcgcc ctttcagcgt gttgcggagg tcgtggcggt tgatctccct
     3061 gtgccccaga aacgaacaga ggccggcata gagcacgcgg gcgtcggggg agagggggag
     3121 cgtccagacc tcgggctcgt accagaactc cccgatcccg gccgcgttat ccggccgacc
     3181 gccgcgcgcg tctcgcagca ggatcacctc gggcaatata gaggtgggtt tcagctcagc
     3241 ccctacagga ccgtgcgcac cgtctcgccg acggcggggg tgtgaatctt gtggtcgggg
     3301 gccagcctga aggcgaggtc gctaatacgg cgcctggagc cgtgtatgag gatgatctgg
     3361 cgcgggctca gcttctcggt gatgccgagg agctcgtcct gcgtgcagtg cgcggcgaag
     3421 cttacgtgct cgacccgcca ctgctcctcc tcaccctctg cgcggtgcgt cccgtaggag
     3481 gcggcggcgt tggagggcag gataacggcg ttctgggcgt gcttctccag gcgcttgcgg
     3541 tagaaggcgc tcgccccacc ctgcatggtt accggcgagg cgatgatggc gcagggattg
     3601 gagaggatgc gctctcgcgc cttgtcgtcg tttgccaccg ccctgatgtt gtcggagaag
     3661 aagatctccc gcgggtcgct gtgctgcagg tagggcttca tgtacccgag ctgctccgcg
     3721 tagagcctct ccgaggtggt aaccacggag ccgtccacgt agatgaagac ctctttgtcc
     3781 aggccgtact ctttggcgta gtgttttata cctaggatga tctcctgcgc ctgcccaagg
     3841 gcgtaggtgg ggatcagaac cgtcccgccc cgctccagcg tggtgtcgtt tattacctcg
     3901 accatggtcc ttatggactc ggcgaagggt tggggcttct ggtcggccag ggtagcctcc
     3961 atgatgagca ggtctatgtc cctcacgtcg ggcagccgtg cggaggggat ggagaagtgg
     4021 tcctccatgc agatgtcccc ggtgtggaac acggtggaag gcccagagtt gaaaagcacg
     4081 ctcgccgccc ccaggatgtg cccgctctcc gtgagcgtaa ccttcgtgcc cccgatgtcg
     4141 agcggctttc cgaagggcac gggtacgagc cgctttctaa cctcgtggat cggcgcccca
     4201 cccggcaggc cgccgcccat ggcggcgtgg tcgttcagcg cgctgactat cagcttggcc
     4261 gacggcggcg tacagtagat cggcacgttc ggcctgtccc tgacgagcaa cggcagcgcc
     4321 ccgcagtggt ccagatgcgc gtgcgtcacc acggccgcgt ctaccccgtt gagcttgctg
     4381 aagtcgggcg tagccggccc gcggccgtcc ggtttgatcc ccgcgtcaac cagtatcttg
     4441 gacttgccga agtcgaggag gtggctgctg ccgccgacct cgccggcccc cccgagcgcc
     4501 tggaaagaca gcgtcggacg gggccttgcg taccctccta ccgcgacgct acgacccgtc
     4561 tccttcgttg ggccggctgg agaaggctcc atgtcgtcgg ggtcctcttc cctggacttg
     4621 ccggggccgc ccagttcggt gtgtgcctgg agcaccttgc ccatgaactc catgaggcgg
     4681 gcggcctggg cggtactctc caccgaggtg ggcagggccg caacctcgtt ctcgatggcc
     4741 cgtatgagga gctcaccaaa gcccctgtcg gtgaagcggg cgttctgcag ggcgttcttg
     4801 atcctatccc gctcctcgac cacgccaccg agctcctcgt gcagctcgtc gacgcgctcg
     4861 gccgccctcg agtaggcccg gcgctcgcct tcgagcgccc gctctacctg gaaccctcgt
     4921 ttttcgaggg tctcgatgcg ctcttcgagc tggccctgca cttcgagtgc cgcggcggcc
     4981 ttctcctcgg ccttcttgac gagctccgag gtgtcggtgc gttcgccggt caggacgtcg
     5041 acctcttcgg cgagcgccgc agccctctcc ttggccgccc tcgaggcgaa agagagccgt
     5101 tcgttctcgc ccttcaaacg ctccagctca gcctccaggt ccctgacgtg cgcgtctttg
     5161 gaggccttct tctcggcctc acccttctcc tcccgccagg catccacaag cgcacccaca
     5221 acgccatcct tctgttcctc ttcggaggcc agggcggcca gcagcaggcc ctctgtggtg
     5281 tgggcgtccc tcgcctcctc caggttctcg agcgtgaggt cctccagcgc cgccgaggtc
     5341 tcaggtggga tctgctcctg tatggccgtg cggaaggcgc cccaggcggc ggcgtcgtgg
     5401 gagattctgc gcgccagccc cttgagcagg ggagcgccgc ctgatttgcg ggtcagggcg
     5461 tcggcctgta tgcccctcgg taggagtacc tcctcgaggc cgggaacgtt cctacagagc
     5521 tcggccagga cgcgcctggt atcgagcctc cccagacgcc gaacgagcaa ttcctcgggc
     5581 agatcctccg gcgggagcct gtcgagcagg ggttcgtcgg cgagctcctc gggggttcca
     5641 gcctcagttc ctgcgtcttt gggtatggga tctgacacgg cggctccctc cacgaatatg
     5701 aatgtcatag agtgtcttca ttcagagccg attttatcac ggcactttga cgccccgacc
     5761 agcctccccc gcacgcccgg ctagctccag ggcgggccgc agggcgggtg ccaggacgac
     5821 ctcgaagcac caggctcgtg gtgcctccta ggcgtggtcg ttatccgaat cggcggtaaa
     5881 ctgtgcggat ggaagggact ccagcgcaag acaacgtcag gatcctggcc cattctccgg
     5941 gtaggtaccc catcctagtg atcgagctac cttctggcga actgcgtacg gcctacctcg
     6001 agaccggcta cgacctcagg cataccaagc ccgtggttag aggctggctc caggacaacg
     6061 ccatcggccg ccacagcttc gtcgcgctag acccccctgt agagtggcca gtagactccc
     6121 tcgcggacta cacgaggaac gagctcctcc agaaaccctg ataccggcct cgcgaagacg
     6181 aaggcaagga gggcgccggt agagaccgcg gccggactca accagaaggg cgcaaacgcg
     6241 ctagcgccgg cccccgcccg ttccatcgcc cctcgaatcc tgcgtccgcg agccaccacc
     6301 ggagccaccg ccgcctgacc cgtcaccgcc ggccccgccg ccgcctgatc caccgccgcc
     6361 tgatccatcg ccacctgatc catcgccacc accggagccg ccaccaccgg agccgccacc
     6421 accggagccg ccaccaccgg agccgccacc gccggagccg ccgccaccgg agccgccacc
     6481 gccggaacca ccgcccccgg agccgccacc gccggagccg ccgccaccgg ag
//
LOCUS       DBNGVD010000357         7143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1540123, whole genome shotgun sequence.
ACCESSION   DBNGVD010000357 DBNGVD010000000
VERSION     DBNGVD010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1540123"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="AC16CM_09550"
     CDS             complement(<1..813)
                     /locus_tag="AC16CM_09550"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3140115.1"
                     /translation="MDDPLVDDAGLPADVPLTAVTRGGLVESVHRGRYAVCDPRGEEL
                     EVRGDPEAYVYLRSSAKPFQALPLVLSGAADAFGLTDEELAIACASHNAEEPHLAAVR
                     SILEKAGLSEDDLQSGAHPPMYGPAAAALGREGQEPRPIHGNCSGKHAGMLAVCAHEG
                     YETLDYREPRHPLQRRILGLLAEVCGVEEDEVLVAGDNCGVPAFALPLRSFATGLARI
                     STGEELPDVIVGAALRIRDAMRSHPFMVAGTGRFDTDLMGRTHLLAKGGAEAV"
     gene            complement(806..1114)
                     /locus_tag="AC16CM_09555"
     CDS             complement(806..1114)
                     /locus_tag="AC16CM_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140116.1"
                     /translation="MSGGGDTAIRMAHLIRVDSYMDFAILSMWTSNPRVDVMLGMLEA
                     SLRGGGPGSGEEDLLQRLRPLVREGRGYLADGDFYSAMGRMRTAHDLLSIHIIRLSDG
                     "
     gene            complement(1111..1932)
                     /locus_tag="AC16CM_09560"
     CDS             complement(1111..1932)
                     /locus_tag="AC16CM_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3140117.1"
                     /translation="MQTVRDIGALTGGAMEPIWSWVKEKIPAGVEVFDAHAHIGVDVD
                     GRTVTADEMRALMDAADVRRSIVFPLNDPNARDDYSGPNEVVWNAYQEYPSSFVPFFR
                     VNPHLDYDVEFARCVERGFKGLKLHPVSQQFELDDPKVVRLLGMAAEIDLPVLIHAGF
                     AMQRIVEPLLPTVEANPGLRLILGHAAMVEVLEATRAFAGHPNVLFETSVVQAKDLYE
                     LCCTMDASRICFGSDIPYGDLPSTLHAILVAADAAGVTEEGLRGIVSENIRRWFP"
     gene            complement(2521..3840)
                     /locus_tag="AC16CM_09565"
     CDS             complement(2521..3840)
                     /locus_tag="AC16CM_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3140118.1"
                     /translation="MRLEVQDPLLSTFNLLVALVLVGLNGFFVAAEFGLVRVRESRLV
                     QLEQEGSTRAAIARQTLRDLDSNLSVCQVGITVASLALGWVGEPAVSHLIEPLLGAVG
                     ITSERVISISAVVLGFTVITYAHLVFGEQAPKYFSIQKAEAVALRISRPLNLFRVMLT
                     PVVYVVNASTNFVLRPWGIKLGEEMEAHSEEELRIMISSSTASGVLDPEERDYLNNVF
                     DFGDRVAREIMVPRPDIEAISSDAELSEMADAAVNGRYTRYPVYEEDLDHILGAVHIK
                     DLFRAARKNPDDFDIRPIIRECLVVPENKRIEEILKEFQTRTLQMAIVIDEWGSVEGL
                     ITIEDVIEEIVGEIQDEFDEDEPAIEEISADLFALDGRIPITEVNDRFELDLPHEDFD
                     TIGGYILGALGRPPETGDTVEADGVSMQVKSVDGPRVSMLTLQRDAG"
     gene            complement(4045..4554)
                     /locus_tag="AC16CM_09570"
     CDS             complement(4045..4554)
                     /locus_tag="AC16CM_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3140119.1"
                     /translation="METNRGSEGILSDELSERREAIVELLKTAYFMELETVMNYVTNS
                     INPDGVRAQEVKESVEEDIQEELGHAQQIAARIKELYGVVPGSLEFKASQDTLQPPDD
                     QVDIVHVIKGVIDAEDGAIQHYTRIVEETEEVDPVTQDMFIAILHDEQGHRRLFEGFL
                     REYEAEGLA"
     gene            complement(4658..5053)
                     /gene="dhaM"
                     /locus_tag="AC16CM_09575"
     CDS             complement(4658..5053)
                     /gene="dhaM"
                     /locus_tag="AC16CM_09575"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIR3140120.1"
                     /translation="MVGLVLVSHSSQIAEGTAELVLQMAGEVEIAAAGGDSDGGFGTD
                     AERIEAAIRGLEADEGLVFMDLGSAVLSAETVLEMLSVEERERVTLVDAPFVEGAFAA
                     GVTASTGADAKDCVEAAMEARTEPKLQEG"
     gene            complement(5053..5685)
                     /gene="dhaL"
                     /locus_tag="AC16CM_09580"
     CDS             complement(5053..5685)
                     /gene="dhaL"
                     /locus_tag="AC16CM_09580"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIR3140121.1"
                     /translation="MDATQATRAVVEEMASVMEENRRLLTRLDSEIGDGDHGNNMNRG
                     FKAALERLDTADPSTPADVLKAVSMALISKVGGAAGPLYGTAFLRASTALGDREEISA
                     EDAAEVIEAALGGIKQRGKAEVGDKTIVDALQPAAEAAKEAAGEGYVVGVFRAAATAA
                     EEGAEATVPLRARKGRASYLGARSEGHQDPGATSTYLLLDAAARTLEGGS"
     gene            6098..>7143
                     /locus_tag="AC16CM_09585"
     CDS             6098..>7143
                     /locus_tag="AC16CM_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3140122.1"
                     /translation="MRQRVSRRNFLKMAGAGLAGAAMVGAAGCGSGPGVSASGAGWKR
                     YSGATINFISENTAPTSAIAANLTPFKELTGINVEIQQLELTSLAQRVALDFGSRSGE
                     YHVIYADAYQVMAPLYKGFTDLNTFIDNPKLPSVPKGTGDFIPTQLDAAGRFEGKLFA
                     LPYDAPTLIWCYRKDLFEKYRDRMEADLGFDPMPSKNSTWDQYYEIARWFNENQDDVP
                     YGAGQQAKQHDSLMCDFSNILWAHGGDYFENGQEVGKQGTTAPGPCTLDSPEAIEAAT
                     FYNKLIQVAHPGSTSWDWNDLGEAFGAEQCAMCAEWHEFAAAWEAGGLMGKVGYAPLP
                     RGPARSANMYGGTG"
BASE COUNT         1261 a         2369 c         2232 g         1281 t
ORIGIN      
        1 tacggcctcc gccccgccct tggcgaggag gtgggtacgg cccatcaggt cagtgtcgaa
       61 gcgtcccgtc cccgcgacca tgaacggatg ggagcgcatc gcgtccctga tgcgcaaggc
      121 ggccccgaca atcacatcgg gcagttcctc accggtagag atgcgggcga ggcccgtggc
      181 gaacgaacgc aggggcagcg cgaacgccgg gacgccgcaa ttgtcgccgg cgactagcac
      241 ctcgtcttcc tccacgccgc agacctcggc caggagcccg aggatgcgcc gctgcaacgg
      301 gtggcgcggc tcgcggtagt cgagtgtctc gtagccttcg tgcgcgcaga cggcgagcat
      361 gccggcgtgt ttgccggagc agtttccgtg tatgggacgc ggctcctgtc cctcccggcc
      421 cagagccgct gccgccggcc cgtacatcgg ggggtgggcg ccactctgaa ggtcgtcttc
      481 ggagagtccg gccttctcga ggatggacct tacggcggcg aggtggggct cctcggcatt
      541 gtgcgaagca caggctatgg cgagttcctc gtccgtaagg ccgaaagcgt ccgcggcccc
      601 ggacaggacg agcggcaggg cctggaacgg tttggccgac gagcggaggt agacgtacgc
      661 ttcgggatct ccccgaacct cgagttcctc gcccctgggg tcgcaaacgg cgtagcggcc
      721 gcggtggacg ctttcgacca gcccgccgcg cgttacagct gtgagcggga cgtccgccgg
      781 taggccggcg tcgtccacca agggatcatc catccgagag gcggatgatg tgaatggaca
      841 gcaggtcgtg ggccgtgcgc atcctgccca tagcggagta gaagtcgccg tcagccaggt
      901 aaccgcggcc ctctctaacc agagggcgta gcctctgtag aagatcctct tccccagagc
      961 caggcccccc gccccgcagg ctcgcctcga gcatgcccag catgacgtct actctgggat
     1021 tcgaagtcca catcgagagg atagcgaagt ccatatagct gtccaccctg atgaggtgcg
     1081 ccattctgat cgccgtatcg ccacccccgc tcacgggaac caccgcctga tgttctcgga
     1141 tacgatgccg cggaggccct cctcggtcac gcctgcggcg tcggccgcga ccaggatggc
     1201 gtggagcgtc gagggaaggt cgccgtaagg gatgtcggag ccgaagcaga tcctggaggc
     1261 gtccatggtg cagcacagct cgtacaggtc cttggcctga accacggagg tctcgaagag
     1321 cacgttggga tggcccgcga aggcccttgt cgcctcaagc acctcaacca tcgccgcgtg
     1381 gcccaggatc aggcgcagtc ccgggttcgc ctcgacggtg ggcagcagcg gctcgactat
     1441 gcgctgcatg gcgaagcccg cgtgtattag cacgggcagg tcgatctccg cggccattcc
     1501 gagcaggcgc accactttcg ggtcgtctag ctcgaactgc tgggagacgg gatgcagctt
     1561 gaggcccttg aacccgcgct cgacgcaacg ggcgaactcg acgtcgtagt cgaggtgcgg
     1621 gtttacccgg aagaagggca cgaaagagct cgggtactcc tggtaagcgt tccagaccac
     1681 ctcgttcggc cccgagtagt catcgcgggc gttgggatcg ttaaggggaa aaactatgct
     1741 tctcctgacg tccgcggcgt ccatgagggc ccgcatctcg tcggccgtaa cggtgcgacc
     1801 gtccacatcg accccgatgt gggcgtgcgc gtcgaagacc tcgacgcctg cggggatctt
     1861 ctccttaacc cacgaccaga tgggctccat agccccgccc gtcaacgccc ctatatcgcg
     1921 taccgtctgc aacccgaccg ccgatctaat cccgacatgg caaccaacaa ccgtgaaatg
     1981 ttatcagggc ggatagatgg ctgcccgacg aacgccacga agggcccagc gtacgccccg
     2041 tcgcgcggga gcacggcccc gagggacgct ctggccctac acctttcgga tcattccggg
     2101 cgcggtgtgt caccgatcgc atgtggaaga tgtcctctcg agtagaacgt ctgccaagca
     2161 ccggcgcgtc ccccatgggt cacgcctata actagccagg cccgcgacca ccctccagct
     2221 gcccgagttg ccaacgcggt agcttccgaa gtcgtaaatc gaactaccct gaccaccgtg
     2281 gattcccgct ttcgcgggaa tgacggatgg aggggaccag gaacgcatcc gtacccggac
     2341 cgcgagaacc agcacatatc gtttcgaaaa gcgcagttct ccagccgaaa gcggggcgaa
     2401 gcccgagcgt tgccgacaag cgacagccga taagctggag cgtgctgaca agccgccgta
     2461 gcggagcgga gccggcgtgc tgacaagcgg agcccgaagg gcggagcgtg cgcggaagcg
     2521 ctagccggcg tccctttgga gggtgagcat cgacacgcgg gggccgtcaa cgctcttgac
     2581 ctgcatggag accccgtcgg cctcgacggt atcgccggtc tccggggggc gtccgagggc
     2641 gcccagaatg tagccgccta tggtatcgaa gtcctcatgc gggaggtcga gctcgaaacg
     2701 gtcgttgacc tccgtgatgg ggatgcggcc gtccagggcg aagaggtcgg cgctgatctc
     2761 ttcgatagct ggctcgtctt cgtcgaactc gtcctgtatc tcgcccacta tctcctcgat
     2821 cacgtcctcg atggtgatga ggccctccac gctcccccac tcgtcgatga ctatggccat
     2881 ctgcagggtg cgggtctgga actccttgag gatctcctct atgcgcttgt tctctggcac
     2941 cacaaggcac tccctgatga tgggccttat gtcgaaatcg tccgggttct tgcgggccgc
     3001 cctgaagagg tccttgatgt gtaccgcgcc gaggatgtgg tcgaggtcct cctcgtagac
     3061 ggggtagcgt gtgtagcgtc cgttcacggc ggcgtcggcc atctccgaga gctcggcgtc
     3121 gctcgagatt gcctctatgt ccggtctggg gaccatgatc tcacgcgcca ccctgtcacc
     3181 gaagtcgaag acgttgttca ggtagtcgcg ctcctctggg tcgagcacgc cggaggctgt
     3241 ggaggaggag atcatgatcc tcagctcctc ctcggagtgg gcctccatct cctcgccgag
     3301 cttgatgccc cagggcctga gcacgaagtt ggtcgaggcg ttgaccacgt aaaccaccgg
     3361 ggtgagcatc accctgaaca ggttcagggg gcggctgatc cggagcgcga ccgcctcggc
     3421 tttctggatg gagaagtact tgggtgcctg ctcgccaaag acgaggtgcg cgtaggttat
     3481 gaccgtgaag ccgagcacca cggcgcttat gctgatcacc cgttcgctgg tgatccccac
     3541 ggcgcccaag agaggctcga tgaggtgcga gacggctggc tcaccgaccc agccaagcgc
     3601 gaggctggcg accgtgatgc cgacctggca cacggagagg ttggagtcga ggtcgcgcag
     3661 cgtctggcgc gcgatcgccg cccgcgtgct gccctcttgc tctagttgca cgagccgcga
     3721 ctcgcgcacg cgtaccagcc cgaactcggc ggcgacgaag aatccgttca ggcccacaag
     3781 aacgagggcc acaaggaggt tgaacgtgga taacaggggg tcttgcactt ctaagcgcac
     3841 tcaccggcgc tgtagccgcg gggtcttcgt cggtgggttt tggcgtgaag ttcgtccatc
     3901 gcgttacggc cagattatac cctagccaca ccactcgcaa cacggaaggg ccgcgatctc
     3961 ttgtgaccgc ggccctttca ggctatcagc tttcggctat cggccatcag caacgggggc
     4021 tgaacgctga cagcatgggg ctatttaagc caggccctcg gcctcgtact cacgcaggaa
     4081 gccttcgaag aggcgcctgt ggccctgctc gtcgtgcaag atggctataa acatatcctg
     4141 ggtaacaggg tcgacttctt cggtctcctc gacgatgcgc gtgtagtgct gaatggcacc
     4201 gtcctcggcg tctataacgc ccttgatgac gtggacgatg tcgacctggt cgtcgggcgg
     4261 ctgcagggtg tcctgggatg ccttgaactc tagcgagccg ggcaccacgc cgtagagctc
     4321 cttgatgcga gccgcgatct gctgggcgtg accaagctct tcctggatgt cctcttctac
     4381 gctctccttg acttcctggg ccctcacgcc gtccggattt atagagttgg ttacgtagtt
     4441 catgaccgtc tccagctcca tgaaatacgc cgtcttgagc agctctacga tggcctcgcg
     4501 cctctccgac aactcatcgg aaaggatgcc ctcgctacct cggttcgtct ccatttttcg
     4561 tggcctcctc atattagagt actacccgta aaacttaccc tggcacgccc cgattaaacg
     4621 taacgtagaa cacgggaacg cggcttcagc ccgaggctca accctcctga agtttcggct
     4681 cggttcgggc ctccatcgcc gcctccacgc aatccttggc gtcagcaccc gtggaggccg
     4741 ttacaccggc ggcgaaggcc ccttctacga acggggcgtc gaccagagta acccgctctc
     4801 gctcctccac ggacagcatc tcgagcacgg tctcggccga gagcacggcg ctgcccaagt
     4861 ccatgaacac gagtccctcg tcggcttcca ggccacggat ggctgcctct atacgctcgg
     4921 cgtccgtgcc gaagccgccg tccgaatcgc ccccggcggc ggctatctcg acctcaccgg
     4981 ccatctgcag gaccaactcg gcggtcccct cggcaatctg gctgctgtgc gagacgagca
     5041 ccagccccac catcaggagc ccccttccag cgtccgcgcc gccgcgtcga ggagcaggta
     5101 cgtggaggtg gcgccggggt cctggtgccc ctcggacctc gccccgagat agctcgcgcg
     5161 ccctttcctg gccctcagcg ggaccgtggc ttctgctccc tcttccgctg ccgtggcggc
     5221 ggccctgaac acgccgacca cgtacccttc gccggcggcc tccttcgcgg cctcggcggc
     5281 cggctgcagg gcgtcgacga tcgtcttgtc ccccacttcc gccttgccgc gctgcttaat
     5341 cccaccgagg gcggcctcga taacctccgc agcatcctcg gcggagattt cctccctatc
     5401 gccgagcgcg gtcgaggcac ggaggaacgc cgttccgtag agtggtcccg ccgcgccgcc
     5461 cactttgctg atcagggcca tcgacacggc tttgagcacg tcagcagggg tcgaagggtc
     5521 ggctgtatcg aggcgctcca gggcggcctt gaaacccctg ttcatgttgt tgccgtggtc
     5581 cccgtcgcct atctccgagt caagtctggt caacagccgc cggttctctt ccataaccga
     5641 ggccatctcc tccaccacgg cacgggttgc ctgcgtagcg tccaaaacct actcctcact
     5701 ctcgcctggt tctgcccttg ccccgagcat cttcccgagg gcgccgcgcg cggaagccct
     5761 ggttatggaa gccggggtcc tcttgctcgc gcacatcgat tctccttcga ctctgtaggc
     5821 cggtctcctt gaagtagggt acatccaccc tcgccgcccg cgcaacgggg gcgagctacg
     5881 ctcctggtcg gacaatctga ccgcggttga cacgcgtcgc ctttccccct atgctcgcca
     5941 caagcacggg ggatggcgag attccaacag gggctttggc aacagcgaca cgacgcgtag
     6001 agagctactc tgcactagct tctcaagcac gctgtcttgt ctgcgtttgc caccatcgat
     6061 gcccgtggcg gcaccttacc gggaaagggg agttgtgatg cgccagaggg tctctcgcag
     6121 gaattttcta aagatggccg gcgccggcct cgccggtgcc gctatggttg gggcagctgg
     6181 ttgcggctcc gggccaggtg tcagcgccag cggcgcggga tggaagcgct acagcggggc
     6241 aaccatcaac ttcatctccg agaacacggc cccaacctcg gccattgccg ccaacctcac
     6301 accattcaaa gagttgaccg gcatcaacgt ggagatccag cagctcgagc tcacctcgct
     6361 cgcccagcgg gtggccctcg acttcggttc ccgcagcggc gagtaccacg tgatctacgc
     6421 cgacgcctac caggtcatgg cgccgctcta caaagggttt acggacctga atacgttcat
     6481 cgataacccc aaactgccct cggtgccgaa gggaacgggc gacttcatcc ccacccagct
     6541 cgacgccgcg ggacgcttcg agggaaaact cttcgcgcta ccctacgacg cgccgaccct
     6601 gatctggtgc tatcgcaagg acctcttcga gaagtacagg gacaggatgg aggctgacct
     6661 gggattcgat cccatgccat cgaagaactc cacctgggac cagtactacg agatcgccag
     6721 gtggttcaac gagaaccagg acgacgtgcc ctacggcgcg ggccagcagg ccaagcagca
     6781 cgactccctg atgtgcgatt tctcgaacat actgtgggcc cacggcggcg actacttcga
     6841 gaacggccag gaagtgggca agcagggcac gaccgccccg ggtccgtgca cgcttgacag
     6901 cccagaagcc atagaggcag ccaccttcta caacaagctt atccaggtcg cccaccccgg
     6961 cagcacgagc tgggactgga acgaccttgg cgaggccttc ggcgccgagc agtgcgcgat
     7021 gtgtgcggag tggcacgagt tcgccgccgc ctgggaggcg ggaggcctca tgggcaaggt
     7081 cgggtacgcg ccgctaccca ggggcccggc caggagcgcc aacatgtacg gcggcacggg
     7141 cat
//
LOCUS       DBNGVD010000358         5176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_993858, whole genome shotgun sequence.
ACCESSION   DBNGVD010000358 DBNGVD010000000
VERSION     DBNGVD010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_993858"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="AC16CM_09590"
     CDS             <1..823
                     /locus_tag="AC16CM_09590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140123.1"
                     /translation="TGTAGTDGSLVVFWHHGTPNTGAPPEPLFEAAERLGIRWVSYDR
                     PGYGGSTSRKDRNVASAAADVSNVADALGITRFAVMGHSGGGPHALACAALLPERVLG
                     VVSVSGLAPYGAGGLDYFAGMADSSAASLEAATRGRVARERYEATAAAEDDEFGFIAA
                     DLAALEGDWAWFDSVVGPALENGPGPLIDDDLAYVSPWGFDPALVGAPTLILHGGSDR
                     VVPGSHGEWLARHCPRAELWLREEEGHISVLHSGGAALEWLREHAVDDRADLSCR"
     gene            1129..1476
                     /locus_tag="AC16CM_09595"
     CDS             1129..1476
                     /locus_tag="AC16CM_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140124.1"
                     /translation="MLLAVLTASILLLVPGQSVSLMGMEVLVVGVAAYAWIVTIQALH
                     LRNLSTIPSYLRKPLLLRITFAQIATLPNVIAGIALLTIGFGGLYWLVAGMVLSTLAA
                     MFDAWVLLVEINR"
     gene            1645..2250
                     /locus_tag="AC16CM_09600"
     CDS             1645..2250
                     /locus_tag="AC16CM_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3140125.1"
                     /translation="MAAVAVAATVDCTTGTVCNGTRNGDIMNGTPDADEMHGRAGGDL
                     MRGKGGQDEMEGGLGADRVLGGSGADELVWGGEQIGRTAPYTYPEKGSDRTSGGPGAD
                     RVYGGFGQGGVDLVFGNGADDTIIVAQRGFPEEVGEVKVTREVVDCGTGEDTVYRDKG
                     LDIIANNCEHKRNGYPAMVDASAQQGSSVQGGLIGASPSDE"
     gene            complement(2366..2752)
                     /locus_tag="AC16CM_09605"
     CDS             complement(2366..2752)
                     /locus_tag="AC16CM_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140126.1"
                     /translation="MPERLNGLLADGATALVKESFTGVAATWWWERRVGGGIEICQEF
                     DPGAASRGISARTGREVAEVENTIVRELGLEDAEPVVLTFEVSGETETDRVAALLAER
                     SARPEGLASSLYDRIVEALEGNPRRP"
     gene            2828..3478
                     /locus_tag="AC16CM_09610"
     CDS             2828..3478
                     /locus_tag="AC16CM_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HIR3140127.1"
                     /translation="MLLVKDSMEREITTLSPDETAATALALCRERRIRHLPVLRGGRL
                     VGIVSDRDLRSSTPAFGDPSRAAALQRVLVEDVMAGEVITADPDDPIEQAANTMRERK
                     IGCLPVMQGDELVGIVTATDVMEALVYLVGANEPGSRMEVAVPDRPGALAGAAGVFGM
                     CGINIVSAAMGATREPEEEGDERERVVVFRVDAIDTNEVVGYLEEAGYAVLWPPRP"
     gene            3475..4635
                     /locus_tag="AC16CM_09615"
     CDS             3475..4635
                     /locus_tag="AC16CM_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HIR3140128.1"
                     /translation="MSGRAAIVHDLALEDYGFGGDHPFNPLRLRLALELCEASGLLED
                     YPFIPSGPATEGDLITVHNLTYVRMVQRAGREAAALSDLRPYGLGTSDNPLFPRMHEA
                     CARVVGGVLAACRLVMAGEVEHAMCISGGLHHAMRSRASGFCVYNDAGVAIARLKQEH
                     PGIRIAYVDTDAHHGDGVQWMFYDDPEVLTISMHESGRYLFPGTGEVREKGSNGGAGF
                     CVNVPLEPYTDDESWISCFEAVVPDALRAFGPDLILSQNGCDGHALDPLTHLFATTRL
                     YEHVPRRMHDLAHELCGARWVATGGGGYDIWRVVPRAWTALWAAVSHQQMPERVAESW
                     RTKWGPKSPVELPALMRDEPEDYPPRPRAVRIAGRNERTVNEVRDRVLPGIR"
     gene            4729..5040
                     /locus_tag="AC16CM_09620"
     CDS             4729..5040
                     /locus_tag="AC16CM_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3140129.1"
                     /translation="MHSVAIPSASSTETRPGIEIEHSTTEEPPYKVILHNDDYTPMEH
                     VVAVLQKVIPRMSVQRAVDIMMEAHNRGKAVATKCHKELAELYQEGLNNEGLIASIEP
                     D"
BASE COUNT          906 a         1650 c         1756 g          864 t
ORIGIN      
        1 cacgggcacg gcagggacgg acggcagtct cgtggtcttc tggcaccacg gcacccccaa
       61 caccggcgct cccccagagc ctctgttcga ggctgcggaa cggctcggca tcaggtgggt
      121 atcgtacgac cgtcctggct acggtggatc tacgtcccgc aaggaccgaa acgtggcgtc
      181 ggccgccgcc gacgtctcta acgttgccga cgcgctgggc atcacccggt tcgcggtcat
      241 gggacactcc ggcggcggac cacatgccct cgcctgcgcc gcgctgctac cggagcgtgt
      301 actcggcgtg gtcagcgtgt ctgggctggc cccatacggg gccggggggc tcgactactt
      361 cgcgggcatg gcggactcca gcgcggcgtc gctagaggcc gccacacggg ggcgagtggc
      421 cagggagcgt tacgaggcga cggccgcggc cgaggatgac gagttcgggt tcattgcggc
      481 ggatctcgcc gcgctcgaag gcgattgggc gtggttcgat agcgtggtcg ggcccgcttt
      541 ggagaacggt ccgggccctc tgatagacga cgatctcgcc tacgtctccc cgtgggggtt
      601 cgaccccgcg ctggttggcg ctccgaccct gatcctgcac ggcggcagcg accgggtggt
      661 gccgggttcg cacggcgaat ggctcgcccg tcactgtccc agggcggagc tctggctgcg
      721 tgaggaggaa ggccacatct cggtcctcca ttccggcggg gcggccctgg agtggctccg
      781 cgagcacgcc gtcgatgatc gagcagacct ttcctgccgg tgagagaact gtcaatagta
      841 catcttgagc acgatcaccg gtcgaaaagt gacggggctc gctgcgcgag ggggccaggt
      901 ggcctggctt gccaggggat gcgccgtgcg gtcgattcgc ctagacttcc gtcgggacca
      961 cgacgaaggg cgggggcaga tggaacagtg gcacgacttt ttcctggcta cgtccgcggc
     1021 agccggagtt ctcaccgggc tcgtgttcgt cggggtctct atcaacctgg aaaagatcgt
     1081 ctcgcagtcg gacagcggac ttccggcagg gccgcggagg ccctgatcat gttgttggcc
     1141 gtacttaccg cctctatctt gctgctcgtg cctggtcaaa gcgtctctct catggggatg
     1201 gaggtgcttg tggtgggggt tgcggcttac gcgtggatcg tcaccatcca ggcgcttcac
     1261 ctgcggaacc tttcgacgat accatcctac ctgcgaaagc ccctgctact acgcataacg
     1321 ttcgcccaga tcgctaccct gcccaacgtg atcgccggca tcgcgctgtt gacgatcggg
     1381 ttcggtggcc tgtactggct ggtggccggc atggtcctct ccacgctcgc ggcgatgttc
     1441 gacgcctggg tgctgctggt cgagatcaac aggtaagcgg ggtgtggcga acgcccgaga
     1501 tctcacccat ttggacgatc ccccaccacg agcgagttgc taacatctac ccatcagtaa
     1561 gaggccaccg aggacgttag gaggaacagt cgatgaagcg tagcctgctt ggactggtag
     1621 tgatcatggc ccttggtgct cggcatggcg gccgtagcgg tggccgccac cgtagactgc
     1681 accaccggca ccgtatgtaa cggtactcga aacggcgata tcatgaacgg cacgcccgac
     1741 gctgatgaga tgcacggccg cgccggcggg gacctcatgc gtggtaaagg cggccaggac
     1801 gagatggagg gcggtctggg tgccgacagg gttctcggcg gttcaggtgc cgatgagctc
     1861 gtgtggggcg gagagcagat cggcagaaca gctccctata cctaccccga aaagggcagc
     1921 gacaggacct ccggtgggcc cggcgccgac cgggtctacg gtggattcgg acagggtggc
     1981 gtggacctcg tctttggaaa cggtgccgac gacaccatca tcgtggcaca gagaggcttt
     2041 cccgaagaag tcggcgaggt caaggtcacc agggaggtgg tggactgcgg taccggggag
     2101 gacactgttt accgcgacaa gggcctcgac atcatcgcca acaactgcga gcacaagagg
     2161 aacggctatc ccgcgatggt agatgcctcc gcacaacaag gctcttcggt acagggaggg
     2221 ctgatcgggg cttcgccttc cgacgagtag gccaaggact cgacagagca agaacggtgg
     2281 gcggggctga cgacggccca gcccctctgg cccggatcgg ccggatgtgt tgccggggca
     2341 agtagaggta ccggaccaga ccggactacg gccttcgcgg gttcccctct agcgcctcca
     2401 cgatccggtc gtagaggctg gaagcgaggc cctcgggccg tgcagaacgt tcggcgagca
     2461 gcgccgccac ccggtccgtc tccgtctcgc cggaaacctc gaaagtaaga accacgggct
     2521 cggcgtcttc gaggccaagc tcacgtacga tggtgttctc cacctccgct acctcgcggc
     2581 cggtgcgggc ggagatcccg cggctcgccg ccccggggtc gaactcctga cagatttcta
     2641 tgccgccccc cacgcgccgc tcccaccacc aggtggcggc gacaccggtg aagctctcct
     2701 tgacgagagc cgtcgcgccg tcggcgagca gcccgttgag cctttctggc atggtccttt
     2761 tataccagcc acgggcttac gagcgtggcc gcgctacagt acaatgcggg tgggaaggag
     2821 gaatccgatg cttctcgtga aagattcgat ggaacgtgag atcacaacgc tctcaccgga
     2881 cgagacggcg gcgacggccc tcgccctgtg ccgggagcga cgcataaggc acctccccgt
     2941 cctgcggggg ggacgtctcg tgggtatagt ctcggacagg gacctgcgct cctccacgcc
     3001 cgccttcggc gacccttcca gggccgcggc cctgcagcgg gtcctggtgg aggacgttat
     3061 ggccggcgag gtgataaccg ccgacccgga cgaccccatc gagcaggcgg cgaacacgat
     3121 gcgggaacgc aagatcggct gcctgccggt catgcaggga gacgagctgg tcggcatagt
     3181 caccgccacc gacgtaatgg aggcgctggt ctacctggtt ggcgccaacg agcccggtag
     3241 ccggatggag gtcgccgtgc cggataggcc gggggccctg gccggcgccg ccggggtctt
     3301 cgggatgtgt ggcatcaaca tcgtaagcgc cgcgatgggg gcgacgaggg aaccggaaga
     3361 agagggagat gagagggagc gcgtggtcgt cttcagggtc gacgccatcg acaccaatga
     3421 ggtggttggg tatcttgagg aggcggggta cgccgtgctg tggccgccta gaccttgagc
     3481 gggcgtgccg ccatcgttca cgacctcgcc ctcgaagatt acgggtttgg cggcgatcat
     3541 cccttcaacc ccctgcgcct ccgtctcgcc ctcgagttgt gcgaggcatc ggggctgttg
     3601 gaagactacc cgtttatccc ctcggggccg gcgacggagg gggacctgat cacggtccac
     3661 aacctcactt acgtgcggat ggtccagagg gccgggcggg aggcggcggc gctctccgac
     3721 ctgaggcctt acggcctggg gacctcggac aatccgctct tcccccgcat gcacgaggcg
     3781 tgcgcgcgcg tcgtcggcgg cgtacttgcc gcgtgccgcc tcgtgatggc gggagaggtg
     3841 gagcacgcca tgtgcatctc gggaggactc caccacgcca tgcgctcgcg cgccagcggt
     3901 ttctgcgtat acaacgacgc cggcgtggcc atcgcgcgcc tcaagcagga gcatcccggc
     3961 atccggatcg cctacgtcga caccgatgcc caccacggcg acggggtgca gtggatgttc
     4021 tacgacgacc cggaggtcct gaccatctcc atgcacgagt ctggccgcta cctctttccc
     4081 ggcacgggcg aggtaagaga gaagggcagc aacgggggcg ccggcttctg cgtcaacgtg
     4141 cccctcgagc cctacacgga cgacgagtcc tggatctcct gcttcgaggc cgtcgtcccc
     4201 gacgccctca gagccttcgg gcccgacctg atcctctccc agaacggttg cgatggtcac
     4261 gccctggatc ccctgaccca cctcttcgca acgacccgcc tctacgagca cgtcccccgc
     4321 aggatgcacg acctcgcaca cgagctctgc ggcgcccgct gggtggccac gggaggcggg
     4381 ggttacgaca tctggcgtgt cgtaccgcgc gcctggaccg cgctctgggc ggcagtctcc
     4441 caccagcaga tgccggaacg ggtggccgag agctggcgca cgaaatgggg cccgaagagc
     4501 ccggtggagt tgcccgctct catgcgcgac gaacccgagg attatccccc gaggccgcgg
     4561 gccgtgagga tagcggggcg taacgagcgc actgtaaacg aggtccgtga cagggtgctg
     4621 cccggcatcc ggtagccaac agagggttgc gaactcggct tcgccgcggt attttgccag
     4681 ggtgatcagt cagtcagacc ggagatgaca gtgcaaagca taccaacgtt gcatagcgta
     4741 gcgatcccct ctgcctcttc caccgagacc agaccgggga tagagataga gcacagcacc
     4801 accgaggaac caccctacaa ggtcatccta cacaacgacg actacactcc aatggagcac
     4861 gtcgtcgccg tactgcagaa ggtcatcccg cgcatgtcgg tacagcgggc cgtggacatc
     4921 atgatggagg cccacaacag gggcaaggcc gtcgcaacca aatgccacaa ggagctcgcc
     4981 gagctctacc aggaaggcct caacaacgaa ggcctaatcg cctccatcga gcccgactag
     5041 gagggcttaa ccgccgatct ggctcattgt gcggtggcgg gcttcctctg gaagcgtcaa
     5101 accagcggcg ggcttgatgc gcgccgtctc ccagagcacc ttgtggatcg ccgggatggg
     5161 gtcttccgag cggata
//
LOCUS       DBNGVD010000359         6199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4454529, whole genome shotgun sequence.
ACCESSION   DBNGVD010000359 DBNGVD010000000
VERSION     DBNGVD010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4454529"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16CM_09625"
     CDS             complement(<1..242)
                     /locus_tag="AC16CM_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR3140130.1"
                     /translation="MSETEKITINLGLVDLGQVDLLVQEGFYSNRTDFIRTAIRNQLA
                     KHAEEVKQTVARKTLVLGLEHYARRDLEAVREAGETL"
     gene            429..1319
                     /locus_tag="AC16CM_09630"
     CDS             429..1319
                     /locus_tag="AC16CM_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3140131.1"
                     /translation="MEVLISGATGLIGSELTPELEAKGHMVRRLTRHPRREGDVRWDL
                     DAGTIDGDLSGIGAVVHLAGESIAEGRWTEEKKRRILESRQKGTRLLAEAVAELDEPP
                     SVMVSVSATGYYGDRGNELLTEESGSGDLFLSEVCREWEAAADPARQAGIRVVHPRLG
                     IVLSTEGGALGATLPIFKLGGGGKIGSGGQYWSWVSFDDVVGAIIHMMETEDLSGPVN
                     VVTSDPPTNAEYTRVLGKVLNRPTFFTVPAPAARLMLGELTDELLLASARVEPVRLQE
                     TGYPFRHPELEGALRHLLGR"
     gene            complement(1335..2258)
                     /locus_tag="AC16CM_09635"
     CDS             complement(1335..2258)
                     /locus_tag="AC16CM_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3140132.1"
                     /translation="MRFGLQVETYGGPGDENAFDRMLAVARLAEEEGFESVWYEDHLS
                     LSHEEDPATPWPQLECLTTLSALAASTRKVGIGSLVACAPYRNPALLAKAWTTLDVVS
                     HGRAIAGIGAGWNKREFRAYGYEFGTVEERMQTLEDTARILDEMMRHSPASYAGRRLS
                     IHQARNEPPPVQRPRPPILIGGNGERRTLRLVARHADMCNVYGSPEEVERRFAALRRH
                     CEEAGRPYEEIVRTINYWALLAADEGGKDERQRRFPTAFSVDTPEETVATLRAYENAG
                     TQYVIVKILDAADLAPVRLFAREVMPAFEPR"
     gene            complement(2275..3321)
                     /locus_tag="AC16CM_09640"
     CDS             complement(2275..3321)
                     /locus_tag="AC16CM_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR3140133.1"
                     /translation="MSRTLGIVGVGLIGGSVGLAARESGWEVVGVDSPQVLEEAERIG
                     AINRASTLEEVRGADLVVLAAPISRVTSLIEDLAPIDALVTDVASAKGAIVNAAQARK
                     LRFVGGHPMAGSQLSGVTNARANLFVGARYFLTPTDLTTPDDYREVSGFVRQLGAVPT
                     AVDPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFRDLTRVGASNPELW
                     SDILAENAPALGQALGAFAGAMAQLGSEIQDRRAIEERFLQARDAYDALGGILVEKDG
                     TNLDVAIPVENRPGVFAEVTTLMGSNAINILDLYVRHSNTERAALVLTLDSDDAPEAR
                     NLLHEAGFGAEFQD"
     gene            complement(3318..4310)
                     /locus_tag="AC16CM_09645"
     CDS             complement(3318..4310)
                     /locus_tag="AC16CM_09645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3140134.1"
                     /translation="MAAEGTDDSYAKLTSNELAFGPLPEAEAALSEVLPRANRYPDSH
                     ASRLRQVVADSNPGTETRHVLVGNGSSEVLLNVLQLLPLPGEVVYGWPSFSLYPSVCA
                     VLGMTPREVPLTEEQEIEPEALLSAVTDRTRAVILCNPNNPSGTFVGLDGVRELADAL
                     PERVVLIVDEAYREFVDDPSYEESHTLALERDNVVIARTFSKAHGLAGLRVGYGIASE
                     HIADYAERVRFPVSVNLAAQVAATASMHAREKVTARAEFVIRERNRLQDAFQEADLEF
                     IPSQGNFVMVRFGAADFERSGVLVREGEALGYPGWSRVTVGDSGENDRVIAALA"
     gene            4564..4860
                     /locus_tag="AC16CM_09650"
     CDS             4564..4860
                     /locus_tag="AC16CM_09650"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3140135.1"
                     /translation="MTDAETQEKVRELRERVDSVDLELIEALSERARIVQEIMALKAE
                     AGAPVYDPRREEDILQSVVERNPGPIYDSSMRDIFELILHRIRDLEIQRGEFPR"
     gene            4862..5674
                     /gene="aroF"
                     /locus_tag="AC16CM_09655"
     CDS             4862..5674
                     /gene="aroF"
                     /locus_tag="AC16CM_09655"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3140136.1"
                     /translation="MDVSTGVKTDVKTTNGMIRPGTFRVIAGPCTVESYEQFVSSAKA
                     VKEAGFSYVRGGAFKPRTSPYSFQGLGEEGLKILSEVSGDLGLKVVTEVMDPYDIELV
                     MEHAEVLQVGARNMANFSLLRRIGAAIAGTDHGVVLKRGLSATVEEWLLAAEYVTAAG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     AVAAEADGLMVESHHDPQEALCDGEQALPVEALMGMKDTLQPFASAMGREVI"
     gene            5701..>6199
                     /locus_tag="AC16CM_09660"
     CDS             5701..>6199
                     /locus_tag="AC16CM_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3140137.1"
                     /translation="MRRLGDRFVLVRELGPGGMARVFLGRDEVLDRPVAVKVVEPDPE
                     DPEVGLRFRREGRAAARLSHPNIVRVYDAGEDELDGNRVSYIVMEYVPCGDFKDMMER
                     TGPLPEAMLSRVGIDVSAGLSQAHERGIVHRDVKPRNILIDERGSPKLADFGIARALD
                     ATTSHN"
BASE COUNT         1066 a         1964 c         2096 g         1073 t
ORIGIN      
        1 agggtctcgc cggcctcacg cacggcctcc agatcccggc gcgcgtaatg ctccaacccg
       61 agcaccagcg tcttgcgcgc caccgtctgc tttacctcct cggcgtgctt cgccaactgg
      121 ttgcggatgg ccgtccggat gaagtccgtg cggttggagt agaagccctc ctgcactagc
      181 aggtccacct gccccaggtc cacaagccca aggttgatcg tgatcttctc cgtctcgctc
      241 atgccaacgt cctatcgtac gaacctcatc gaacatccat ataccatcca gatggatggt
      301 aacaccccag ggcccggttc cgtcaagcac ttcgtgtggg gaggctcggg ctgtgggaga
      361 cttcatacgg tttcgtgggg cgtcgggcat taccatggtc tgtggtaggt agatgcggga
      421 ggaggaacat ggaagttctg atcagtggag cgacggggct catagggtcg gagttgaccc
      481 ccgagcttga ggcgaagggt cacatggtca ggcgtcttac gcggcatcct cgtcgggagg
      541 gggatgtgcg ttgggatctc gacgccggca ccatcgacgg ggatctctcc ggcatcgggg
      601 cggtggtcca cctcgccggc gagagcatcg cggaggggcg ttggacggag gagaagaagc
      661 ggcgcattct ggagagccgg cagaagggca cgcggcttct tgcggaggcc gtagctgaac
      721 tagatgagcc gccctccgtg atggtctctg tctcggccac cgggtactac ggtgacaggg
      781 gaaatgagtt gctcacggag gagagcgggt cgggggatct cttcctctcg gaggtctgcc
      841 gggagtggga ggcggcggcg gatccggcgc gccaggcggg gattcgggtc gtgcacccca
      901 ggctcggcat cgtgctcagc accgaagggg gggcgctcgg tgccacgctt cccatattca
      961 agctcggcgg gggagggaag ataggatcgg gcggtcaata ctggtcgtgg gtctccttcg
     1021 acgacgtggt cggggccatc atccacatga tggaaaccga ggatctcagc ggccccgtga
     1081 acgtggtaac gtccgatccc ccgaccaacg cggagtacac tagggtgctc ggcaaggtac
     1141 tcaaccggcc cacgttcttc accgtgccgg cacccgcggc acgcttgatg ctcggtgagc
     1201 tcaccgacga gttgcttctg gccagcgcac gggtcgagcc ggtgcggttg caggagacgg
     1261 gatacccctt ccggcatccc gagctcgaag gggcgttgag acacctgctc ggacggtagg
     1321 ggggcgctat acgcttaccg gggttcgaag gctggcataa cctcccgggc gaagaggcgg
     1381 acgggggcca ggtcggcagc atcaaggatc ttcacgatca cgtattgtgt tccggcattc
     1441 tcgtaagctc gcagcgtggc gaccgtctcc tcgggtgtgt ccaccgagaa agccgtgggg
     1501 aaacgtctct gtctctcgtc cttcccacct tcgtcggcgg cgagcagcgc ccagtagttg
     1561 atcgtgcgca cgatctcctc gtaaggtcgg ccggcctctt cgcagtggcg gcgtagcgcg
     1621 gcgaacctgc gctcgacctc ctcgggactg ccgtagacgt tacacatatc ggcgtggcgg
     1681 gcgacgagac ggagggtccg cctctcgccg ttgccgccga tcaggatcgg cggccggggt
     1741 ctctgtacgg gtgggggttc gttccgcgcc tggtggatcg agagccttcg gcccgcgtaa
     1801 gaggcggggc tgtggcgcat catctcgtcg aggattcgcg cggtatcctc cagcgtctgc
     1861 atgcgctcct ccaccgtgcc gaactcgtag ccgtaggccc ggaactcccg cttgttccag
     1921 ccggccccga tgccggcgat ggcacggccg tggctcacaa cgtcgagcgt ggtccaggcc
     1981 ttggccagca gcgccgggtt gcggtagggg gcgcaggcga caagcgaccc gatgccgacc
     2041 ttccgcgtgc tcgccgccag cgccgacagg gtggtaaggc actccaactg gggccagggg
     2101 gtggcgggat cctcttcgtg cgagagcgag aggtggtcct cgtaccagac ggactcgaac
     2161 ccctcctcct ccgcgagcct cgccacggcg agcatcctgt cgaacgcgtt ctcatccccg
     2221 ggaccgccgt aggtctccac ctgcaggccg aaacgcactg ctagagcccc atgttcagtc
     2281 ctggaactcc gcgccgaagc ccgcttcgtg cagcaaattg cgggcttcgg gggcatcgtc
     2341 cgaatccagg gtgaggacca gggcggcgcg ttctgtgttg gagtggcgaa cgtagaggtc
     2401 gaggatgttg atggcgttgg agcccatcag ggtcgtcacc tcggcgaaga cgcccgggcg
     2461 gttctccacc gggatcgcca cgtccaggtt cgtgccgtcc ttctccacca gtatgccacc
     2521 cagggcgtcg taggcatcgc gagcctgcag gaagcgttcc tcgatggccc ggcggtcctg
     2581 tatctcgctc ccaagctgcg ccatcgcccc cgcaaacgcc ccgagcgcct ggccgagggc
     2641 cggggcgttc tcggccagga tgtcagacca gagctcgggg ttcgaggctc ccaccctcgt
     2701 caggtcccgg aaagatggtc cggcgaagga gagggcctcc ggtgagatat ccgaggcgac
     2761 cttcaggagg gccgcggcca tcagatgcgg caggtgcgag agggcggcca tgagcaggtc
     2821 gtgcttctcg gggtcgacgg ccgtggggac cgcgccgagt tgcctgacga acccggagac
     2881 ctcacggtag tcatctgggg tggtgagatc cgtcggggtc agaaagtagc gggcgcccac
     2941 gaagaggttg gcccgggcgt tcgtgacgcc ggagagctgg ctccccgcca tcgggtgccc
     3001 tccgacgaag cgtaacttcc gggcctgtgc cgcgttcacg atggctcctt tcgcgctagc
     3061 aacgtcggta acgagagcgt cgatcggggc gaggtcctcg atgaggctcg tgaccctgga
     3121 tatgggggcg gcgagcacga cgaggtcggc gccgcgcacc tcttccagcg tcgaggcgcg
     3181 gtttatggcg ccgattcgct cggcctcttc caggacctgt gggctgtcta cgcccacaac
     3241 ctcccagccc gactctctgg cggccaggcc gacggaaccg ccgatcaggc cgaccccgac
     3301 gatgcctagc gtgcggctca tgccagggcc gctatgaccc ggtcgttctc gcccgaatcg
     3361 cccaccgtca cccggctcca gcccgggtag ccaagggcct caccctcgcg tacgagaacg
     3421 ccggacctct cgaagtcggc cgcgccgaac cggaccatca cgaagttccc ctgcgagggg
     3481 atgaactcca ggtcggcctc ctggaaagcg tcctgcagcc ggttgcgctc cctgataacg
     3541 aactctgccc gcgccgtcac cttctcccgc gcgtgcatcg aggccgtggc cgccacctgg
     3601 gcggccaggt tcaccgagac cggaaagcgg acgcgctcgg catagtccgc aatgtgctcc
     3661 gaggcgatac cgtaacctac cctcaggcca gccagcccgt gcgccttgga gaacgtgcgg
     3721 gcgattacga cgttgtccct ctccagcgcc agggtgtggg actcttcgta ggacggatcg
     3781 tccacgaact ccctgtaggc ctcgtccacg atcaggacta ccctttctgg caacgcgtcg
     3841 gcgagctccc tcaccccgtc caggcccacg aacgtgccgc tcgggttgtt tgggttgcag
     3901 aggatcaccg cccgcgtcct gtccgtgacg gcagagagga gagcctccgg ttctatctct
     3961 tgctcctccg taagcgggac ctcccttggc gtcatgccaa gcaccgcaca cacagaaggg
     4021 tagaggctga acgagggcca accgtagacc acctccccgg gaagcgggag gagttgaagc
     4081 acgttcagca gcacctcgct cgacccgttg cccaccagca cgtgtcgcgt ctcggtgccc
     4141 gggttcgagt cggcaaccac ctgccggagt cgggaggcgt ggctgtccgg gtagcggttt
     4201 gcgcggggca gcacctccga gagcgcggcc tcggcctctg gaagcgggcc gaatgccagc
     4261 tcgttcgagg tcagcttcgc gtagctgtcg tcggttccct cagccgccat cctccaggat
     4321 ttcggtggaa cgtacgggcg caagggttct agctcttctc taaaggttac aggcatcgca
     4381 aaaggcttca ggcatcaggc atctgctttc aggtcgtgtg ttcgtgtctc aacaactaaa
     4441 gactaaggcc ctttcactga ccttgcaaca ggagtagatt tcggaaccga tcacgattct
     4501 ttgcgatttc ctgcagcctg acacctgtaa cctgtagcct gcgctgatac aattccagat
     4561 cttatgacgg atgccgagac acaggagaaa gtcagggagc taagggagcg ggtcgacagc
     4621 gtcgacctgg agctcatcga ggccctgagc gagcgagcgc gcatcgtgca ggagatcatg
     4681 gcgctcaagg ctgaagccgg agcccctgtt tacgacccca ggcgggagga ggatatcctc
     4741 cagagcgtgg tggagcgaaa ccccggtccc atctacgact cctccatgag ggatatcttc
     4801 gagcttatct tgcaccgcat ccgtgacctt gagatccaac gcggggagtt tcccaggtaa
     4861 gatggacgtt tctacgggcg taaagaccga cgtaaagaca accaacggca tgatccggcc
     4921 aggcaccttc agggtgatag ccggaccttg cacggtcgag agctacgagc agttcgtgtc
     4981 ctcggcgaag gccgtgaagg aggcaggctt cagctacgtg cgaggcggcg ccttcaaacc
     5041 ccgcacctcc ccgtactctt tccagggcct cggcgaggag ggcctgaaga tactttccga
     5101 ggtctcgggg gatctcggcc tcaaggtcgt gaccgaggtg atggacccct acgatataga
     5161 actggtcatg gagcacgcgg aggtcctgca ggtcggggcc cgcaacatgg cgaacttctc
     5221 gctgctgagg aggatcggcg ccgcgatagc gggaacggac cacggggttg tcctgaagcg
     5281 gggtctctcc gcgacggtgg aggagtggct ccttgcggcg gagtacgtga cggccgcggg
     5341 cggggacaac gtggtgctct gcgagcgcgg catccgcacc ttcgagacga gcacgcgctt
     5401 cacgcttgac ctctcggccg tgatcgtggc caagaggctg acggacctgc cggtgatagt
     5461 ggacccgtcc cacgccgccg ggcggcggga cctggtcgtg ccgctctcca aggccgcggt
     5521 cgcggccgag gccgacggcc tgatggtgga gagccaccac gatccgcagg aggccctgtg
     5581 cgacggggag caggcgctcc cggtcgaggc gctgatgggc atgaaggaca cgctgcaacc
     5641 tttcgctagc gccatgggca gggaggtcat ctagtctcgg acgcagcccc cggaggcacg
     5701 atgaggcgcc tcggggacag gttcgtgctc gtgcgcgagc tcggtccggg cgggatggcc
     5761 cgcgtctttc taggccgcga cgaggtgctc gaccggcccg tggccgtcaa ggtggtcgag
     5821 ccagaccccg aagacccgga ggtaggcctg cgtttcaggc gtgagggaag ggcggccgcc
     5881 cggctctccc atcccaacat agtcagggtc tacgatgcgg gcgaggacga gctagacggg
     5941 aacagggtct catacatcgt catggagtac gtcccctgcg gcgacttcaa ggacatgatg
     6001 gagaggaccg gcccgctccc ggaagcgatg ctctcgcggg tgggcataga cgtgtccgcc
     6061 gggctctccc aggcccacga gcggggcatc gtgcacaggg acgtgaaacc ccgcaacatt
     6121 ctcatcgacg agaggggtag ccccaagctg gccgacttcg gcatagcccg ggccctcgac
     6181 gccaccacga gccacaaca
//
LOCUS       DBNGVD010000360         5385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3908833, whole genome shotgun sequence.
ACCESSION   DBNGVD010000360 DBNGVD010000000
VERSION     DBNGVD010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3908833"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16CM_09665"
     CDS             <1..417
                     /locus_tag="AC16CM_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIR3140138.1"
                     /translation="VLTATTAPVALADGIEDLLSPLKKVRFPAHEVAMMMTIALRFIP
                     TLGEEAEKIRGAQAARGADFSEGGPLRRARSLVPVLVPLTVGAFRRADELAEAMESRG
                     YKGGEGRTRYRELRFRARDSLALCATVLVLVVGVLL"
     gene            414..1175
                     /gene="truA"
                     /locus_tag="AC16CM_09670"
     CDS             414..1175
                     /gene="truA"
                     /locus_tag="AC16CM_09670"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3140139.1"
                     /translation="MKYAGLVEYDGAEFAGWAAQPGLRTVEDVLSEALSTVLRQPVKM
                     SVAGRTDAGVHASGQVVSFAARTDLRAAQISYKATAVLPTDVALRRCVEVPDAFDARR
                     DAVARTYEYRVINDEIRSPLRRRHAAFVPRALDLDLLSAAGRIVEGTHDFTAFTPSKG
                     YHVRFERVITDSRWTCRDDLLTYNITADSFLYGMVRTLVGTMLEVAGGKRDLSQFGRL
                     LAGASRSEAGPAVASRGLTLVGVDYDHPDLWRSER"
     gene            1172..1840
                     /locus_tag="AC16CM_09675"
     CDS             1172..1840
                     /locus_tag="AC16CM_09675"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3140140.1"
                     /translation="MNTENTRELLEEIDGYIEDLFGTSDEVLEATLRDSRRGGLPDIT
                     VSPNQGRLLQLLVEISGARRVLEIGTLGGYSAIHLARGLPDDGWLISLELDKHHANVA
                     RNNVERAGLSEVVEIRVGDAHELLNDLAEQDETPFDLVFIDADKEGYPDYLQAGLRLV
                     RPGSLILGDNTIRGGSVLDPQEGTARATREFNRLVAEDPRLHGIVLPLIRERIDGMTI
                     ARVV"
     gene            complement(2030..2452)
                     /locus_tag="AC16CM_09680"
     CDS             complement(2030..2452)
                     /locus_tag="AC16CM_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3140141.1"
                     /translation="MDDCVFCGIVVGRLPASLVWRGDGCTAFMDIQPINAGHVLVVPD
                     EHATSLADVPEDTGARIFRVAQQVTSALYGSGLACEGVNLFLADGEAAGQEVFHVHLH
                     VVPRFEGDGFGLRLGPDYANVPDREELDEAAKSIRDVL"
     gene            2631..3062
                     /gene="rplM"
                     /locus_tag="AC16CM_09685"
     CDS             2631..3062
                     /gene="rplM"
                     /locus_tag="AC16CM_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3140142.1"
                     /translation="MKSYMARPLEVERKWYVVDAEGKHLGRLATEIARVLRGKNKVQY
                     TPHVDVGDFVVVVNADRVVVTGRKAEQRVYRRHSGYPGGMKEMSYEQMLARKPTEILR
                     KAVYGMMPKSRLARKQFKKLKIYAGPDHPHAAQDPQALEVK"
     gene            3063..3455
                     /gene="rpsI"
                     /locus_tag="AC16CM_09690"
     CDS             3063..3455
                     /gene="rpsI"
                     /locus_tag="AC16CM_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3140143.1"
                     /translation="MAEALYIGTGRRKTAVARVRILPGNGTITVNGRELDEYFPRPTY
                     RSTVRSPLELLGTAGRYDVVAKIEGGGPTGQAEALRHGIARALAEESQEARGELKAAG
                     LLTRDDRAVERKKYGLKKARKRPQFSKR"
     gene            3517..4878
                     /gene="glmM"
                     /locus_tag="AC16CM_09695"
     CDS             3517..4878
                     /gene="glmM"
                     /locus_tag="AC16CM_09695"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3140144.1"
                     /translation="MRFEWGKRGGELRGRTIQFGTDGVRGVANKDLLPEDALRLGLAA
                     ARSFGGKIAIGRDTRRSGEMLARALSAGAASGGVDVVDLGVLPTPGVAALAPRLAATA
                     SAVVSASHNPFPDNGIKFFSREGRKLAPEKEAEIEGLIESESLRPTGSGVGSIVGLDD
                     AVEAYTEHVLGRLRPSASGMKVLLDCANGAAYRVAPLVFGELGVDLTLRGVGPTGTNI
                     NDGCGSTHVDELDASGQDVAFAFDGDADRVLAVDEKGGVVDGDRIIAILARDMQERGV
                     LEGGVVVTVMSNLGFFKAMEAMGIPYEVTGVGDRHVAEAMGRLGASIGGEQSGHVIIS
                     EHVTTGDGLITALALLDVMVRSGKPLSELAGVMEKYPQTLINVRVHEPGAAKKVASSG
                     AVIQAVSEAEERLGEEGRVLLRPSGTEPVVRVMVEHEDETVCRRVCEEVSRVVAGAGE
                     EKA"
     gene            4875..>5385
                     /locus_tag="AC16CM_09700"
     CDS             4875..>5385
                     /locus_tag="AC16CM_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140145.1"
                     /translation="MRFVGCALAWRPGEAREKVSALVVIDERGSIIANSFAVSAADIA
                     GAVERYGTDRRGVLVGIDAPLAVPNERGTRKIERILSKVALPAYSASRRMFEGEPLAE
                     EVLAELEKVGVEYTDYPFPRERDQSAVVEVDSVATLKVLSLERSGAAENGDLAQRLRA
                     MDDPKMRKGN"
BASE COUNT          993 a         1678 c         1862 g          852 t
ORIGIN      
        1 gtgctcacgg ccacgacagc ccccgtcgcc ctcgccgacg ggatagaaga cctgctgtcc
       61 ccgttgaaga aggtacggtt tcccgcgcac gaggtcgcga tgatgatgac catagctctg
      121 cgtttcatcc ccacgctggg ggaggaggct gagaagatcc ggggcgccca ggccgcccgc
      181 ggagcagact tctccgaagg gggaccgctc agaagggccc gctcgctcgt gccggttctc
      241 gtccccctga ccgtgggggc cttccgccgc gccgacgagt tggctgaggc gatggagagc
      301 aggggctaca aggggggcga gggaagaacc cgctaccgcg agctacgctt tcgggcgcgg
      361 gattcgctcg cgctctgtgc caccgtgctc gtcctggtcg tgggggtact gctttgaagt
      421 acgccgggct cgtcgagtac gacggggccg agttcgccgg atgggccgcg cagcccgggc
      481 ttcgtacggt cgaggacgtc ctctccgaag ccctctcaac ggtgctgcga cagcccgtca
      541 aaatgagcgt cgccggacgg acggacgctg gggtgcatgc gagcgggcag gtcgtctcct
      601 tcgcggcgcg gaccgatctc agggccgccc agatctccta caaggccacg gcggtgctgc
      661 ccaccgacgt ggcgctcagg cgctgcgtgg aggttcccga cgccttcgac gcgcgcaggg
      721 atgccgtcgc gagaacctac gagtataggg tgatcaacga cgagatccgc tcccccttga
      781 ggcgccgcca cgcggccttc gtgccgcggg ctctggacct ggatctcctc tccgccgctg
      841 gcaggatcgt cgagggcacc cacgacttca cggcctttac gccatcgaag ggctaccacg
      901 tccgcttcga gcgggttatc accgactcgc ggtggacctg tagagacgac ctgctcacct
      961 acaacataac cgccgactcg ttcctctacg gcatggtccg taccctggtc ggtacaatgc
     1021 ttgaggtcgc tggcggcaag agggacctgt cgcagttcgg gcggctgctc gcgggggcaa
     1081 gccgcagtga ggcggggccc gccgtggcga gccgcgggct cacgctcgtc ggcgtagatt
     1141 acgaccatcc tgacctatgg aggagcgagc gatgaacacg gagaacacca gagagctcct
     1201 ggaggagatc gacggctaca tcgaggatct gttcggcact tcggacgagg tcctcgaggc
     1261 cacgctcagg gattcccgcc gcggcgggct tccggacata accgtctcgc ccaaccaggg
     1321 aaggctgctg caattgctgg tcgaaatatc gggggcgcgg cgcgtgctcg agataggcac
     1381 cctgggcggc tacagcgcga tccacctggc acggggcctg cccgatgatg ggtggctgat
     1441 ctccctggag ctggacaagc atcacgccaa cgttgcccgc aacaacgtcg aacgggccgg
     1501 cctctcggag gtcgtggaga tcagggtcgg cgacgcccac gagctactga acgaccttgc
     1561 cgaacaagac gaaaccccct tcgatctcgt cttcatcgac gcggacaaag agggataccc
     1621 tgactacctg caggcgggcc tacggctcgt gcggcccggg tccctgatcc ttggcgacaa
     1681 cacaataagg ggtggtagcg tcctcgaccc gcaagaggga acggcccggg ccacacgcga
     1741 attcaaccgc ctggtggccg aagatcccag gttgcacggt atcgtgctcc ccctcatccg
     1801 cgagcgcata gacggcatga ccatcgcaag ggtcgtgtag gccgtctcgg atgagccggt
     1861 ttcggcgggc ccgtgggata tgcaccaacg ttcgtggcat gcctacgtac ggttatgtat
     1921 tgcgccgcgc cgtgtaagca cgcggattcg aacacgttcg ttcgaccaac ggatggcata
     1981 cgcacgaacc atgtacgggg ggttcgcgaa ccgcccaacg ggcatcgcct cacaatacgt
     2041 ccctgatact cttcgcggcc tcatctaatt cctccctatc gggcacgttg gcgtaatcgg
     2101 ggcctaaccg caggccgaag ccgtccccct cgaaacgtgg aaccacgtgc aggtgaacgt
     2161 ggaatacctc ctggcccgcg gcctcgccgt cggccagaaa caggttgacc ccctcgcagg
     2221 cgaggccgct tccgtagagc gcgctggtca cttgttgcgc gacgcggaag atcctggccc
     2281 ccgtatcctc tggaacatcg gccaggctgg tggcgtgctc gtccggcacc acgagaacat
     2341 gcccggcgtt gataggctga atgtccatga acgccgtgca cccgtcaccg cgccacacca
     2401 ggctcgctgg caatcgaccc accactatgc cgcagaaaac acaatcatcc atgtcgttgc
     2461 catggtggca caacgccgag cgccgcgccg gcccaggatg gcgcttcaga ccgatcccga
     2521 agcctgaaac ctgtagcctg tagccttctc tcggctggtc gttttgccga tgtatgctac
     2581 tatttgatcc gtctaagggc ctttgaagat ttcagaacct ccggaggatg atgaagagtt
     2641 acatggcgcg gccgctcgag gtcgagcgaa aatggtacgt cgttgacgcg gagggcaagc
     2701 acctcggccg actggcgacg gagattgcgc gcgtcctgcg cggcaagaac aaggtacagt
     2761 acacgccgca cgtcgacgtc ggagatttcg tcgtcgtggt gaacgccgac agggtggtgg
     2821 tcacgggcag gaaagccgag cagagggtct acaggcgcca ctctggctac ccgggcggca
     2881 tgaaggagat gagctacgag cagatgctcg cccgcaagcc gaccgagatc ttgcgcaagg
     2941 ccgtctacgg gatgatgccg aagagtcggc tggcccgcaa gcagttcaag aagttgaaga
     3001 tctacgcggg ccctgatcac ccacacgcgg cccaggaccc gcaagcgctc gaggtgaagt
     3061 agatggctga ggctctatat atcgggacag gacgacgcaa gaccgccgtg gcacgggtca
     3121 ggatcctgcc ggggaacggc accatcaccg tgaacggccg tgagctggac gagtacttcc
     3181 cccgcccgac ctaccgctcg acggtacgtt cgccgctgga gctactgggc accgccggcc
     3241 gctacgacgt ggtcgccaag atcgaaggcg gcggcccgac cggccaggcc gaggccctgc
     3301 gccacggcat agcgcgggcc ctggccgaag agtcgcagga ggcccgtggc gagctaaagg
     3361 ctgccgggct gcttacccgt gacgacaggg ccgtggagcg caagaagtac ggtctcaaga
     3421 aggcccgcaa gcgcccacag ttctccaagc gttaacggcg tcgcagcgcg aacgacgcta
     3481 gatcagcgag cccttccaca caccggaagg gctcgtttgc gcttcgagtg gggcaagagg
     3541 ggaggggaac tgagaggtag gaccatacaa ttcggcaccg atggggtgcg cggggtggcc
     3601 aacaaggacc tgctgccgga ggatgcgctg cgactcggcc tcgccgccgc aaggagcttc
     3661 gggggcaaga tcgccatcgg acgcgacacg cggcggtctg gggagatgct cgcccgtgcc
     3721 ctctccgcag gtgccgctag tggcggcgtc gacgtggtgg acctcggcgt cctacccacg
     3781 cccggggtcg cggccctcgc accacgtctt gcggccacgg cctcggccgt ggtaagcgcc
     3841 tcgcacaacc ccttccccga caacggcatc aagttcttct ccagggaggg acgcaagctc
     3901 gcgcctgaga aggaggccga gatagagggc ctcatcgagt cggaatccct ccgcccgacc
     3961 ggaagcgggg tcgggagcat tgtgggactg gacgacgcgg tcgaggccta cacggagcac
     4021 gttctcggac gattgcgtcc atcggcaagc gggatgaaag tactgctcga ctgcgccaac
     4081 ggcgccgcct acagggtcgc cccgctggtc ttcggggagc tgggtgtgga cctcacgctc
     4141 aggggggtag ggcccacggg caccaacatc aacgacggtt gcggttccac gcacgtcgac
     4201 gagctcgatg cctccggaca agacgtggcc ttcgccttcg acggggacgc cgacagggtg
     4261 ctcgcggtcg acgagaaggg cggcgtggtc gacggggacc ggataatcgc catcctcgcc
     4321 agggatatgc aggagcgcgg ggtgctggag ggcggggtcg tggtcacggt gatgagcaac
     4381 ctcggcttct tcaaggctat ggaggcgatg ggcataccct acgaggtaac gggtgtgggg
     4441 gacaggcacg tggccgaggc catgggccgg ctcggggcct ccataggcgg ggagcaatcg
     4501 gggcacgtca tcatctccga gcacgtcacg accggggacg gcctcataac ggccctcgcc
     4561 ctgctcgacg tcatggtccg cagcggtaaa ccgctctcgg aactggccgg cgtaatggag
     4621 aagtacccgc agaccctgat caacgtgcgg gttcatgagc cgggggccgc gaaaaaggtg
     4681 gcatcatccg gggccgtaat acaggccgta tcggaggcgg aggagcggct tggtgaggag
     4741 gggcgcgttc tcttgaggcc cagcgggacg gagcctgtgg tccgggtgat ggtagagcac
     4801 gaagacgaga cggtgtgccg ccgggtctgc gaggaggtct cgcgggtcgt ggccggcgct
     4861 ggagaggaga aggcttgagg tttgtgggct gtgccctggc gtggagaccg ggcgaggcgc
     4921 gggagaaggt ctccgccctg gtggtaatag atgagcgggg gagcattatc gcaaactcct
     4981 tcgccgtgag cgcggcggat atagcggggg cggtcgagcg ttacggcacg gaccgtcgcg
     5041 gcgtcctcgt cggcatcgac gcgccgcttg cggttccgaa cgagcgggga acccgcaaga
     5101 tagaacgtat cctctccaag gtggccctcc cggcctactc cgcgagccgc aggatgttcg
     5161 aaggcgagcc cctggcggaa gaggtactgg ctgagctcga gaaggttggc gtggagtaca
     5221 cggattatcc tttcccgcgg gagagggacc agagcgcggt cgtcgaggtt gactcggtgg
     5281 cgacgctcaa ggtgctctct ctggagcgct cgggcgcggc cgagaacggc gacctggcgc
     5341 agaggctccg cgcgatggac gaccccaaga tgcgaaaggg aaaca
//
LOCUS       DBNGVD010000361         2870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3727537, whole genome shotgun sequence.
ACCESSION   DBNGVD010000361 DBNGVD010000000
VERSION     DBNGVD010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3727537"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..621
                     /locus_tag="AC16CM_09705"
     CDS             19..621
                     /locus_tag="AC16CM_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140146.1"
                     /translation="MDDTASSGSTVVEAVSSGRLGGRVRELRRARGLTLDRLAELSGV
                     SRAMISKLERGEKNPTLVVAARLAEGLGVTLSRLAGMEERREVIILPRERRMVMRDPE
                     TGFERQLLSPGSVGLGVEFIQNKIPRGSTSGEFPPHRRGVDEQIVVERGTLRATLAGE
                     DYLLRQGDSLYFEADVPHRFDNVGDEECTYYLVISTRRPG"
     gene            complement(721..1194)
                     /locus_tag="AC16CM_09710"
     CDS             complement(721..1194)
                     /locus_tag="AC16CM_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140147.1"
                     /translation="MDKPSKASRTLVCPFCSEAELREFGRNSLRCDECGGVLGGALLE
                     TLNKIISLPDAAGGHACECGHPEMRKLPDDVYRCPACGSEVTPISAPVSWKSPDHSEA
                     YWSGWLDGRYGDPQRFTDNRRLARWGSAADRLDYYRGHRAGYETRVSKVRLAEAS"
     gene            complement(1366..2529)
                     /locus_tag="AC16CM_09715"
     CDS             complement(1366..2529)
                     /locus_tag="AC16CM_09715"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR3140148.1"
                     /translation="MSEDRGGGRRFNSATDKEILNADVADSYFHRTMQILRARGLQDT
                     PVHAEIAFKTSDPEAWFVVAGLDEVAYLLEEVDVDVRAVPEGTICRPNEPILTLSGSY
                     GAFAEHETALLGLLCQASGVATGAARCRLAAGQKPVISFGARRMHPAVTPMIERAAYL
                     GGCDMVAVDLAMKRLGIPATGTTPHALVLLIGSTAEAARAFDEVVEPDVPRTIIIDTF
                     DDEKFGALLAARAIPDSVYAIRMDTPGNRRGDFLDLMREVRWELDRNGFGHVKIFASG
                     GIDVDYILHLNPACDAYGVGGAIADAPMIDYSLDLVEVNGEDRSKRGKRGGRKRLLDL
                     PDGTRATVPASSPTPDGAIDVLVPLSEMYSPPPGTDTLRQRVLDQLESGSFGL"
     gene            2638..>2870
                     /locus_tag="AC16CM_09720"
     CDS             2638..>2870
                     /locus_tag="AC16CM_09720"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3140149.1"
                     /translation="MNSTGLNHSFAAAAGTPYAAEAAAETYRAGGNAADAAVAAAAAV
                     GVTEPLMSSIGGGGFALVRDPSGGAELIDYYDT"
BASE COUNT          501 a          881 c          985 g          503 t
ORIGIN      
        1 tagaatttct tctaccaaat ggatgacacg gcttcaagcg ggtccacggt agtcgaggcg
       61 gtctcgtccg ggcgcctagg tggacgggtg agggagctgc gccgggcgcg cggactgacg
      121 ctcgacaggc tggcagaact ctccggtgtg agccgggcca tgatctccaa gctcgagcgc
      181 ggcgagaaga acccgacgct ggtggtcgcg gccaggctag ccgaggggct tggcgtgacg
      241 ctctcgcgtc tcgccggcat ggaagagcgg cgcgaggtca tcatcttgcc gagggagagg
      301 cgcatggtaa tgcgagatcc cgagaccggg ttcgagcgcc agctcctctc accagggtcc
      361 gtcggtctgg gcgtcgagtt catacagaac aagatacccc ggggctccac ctcgggcgaa
      421 ttcccgccac atcgcagggg tgtagacgag cagatagtcg tggagcgcgg gaccctgagg
      481 gcgacgctag caggcgagga ttatctcctc aggcagggcg actcccttta cttcgaggcc
      541 gacgtaccgc accgcttcga caacgtgggc gacgaagaat gcacctacta cctggtgatc
      601 agcacccgca ggccaggatg atggggcaga aggagagctg gtacctggcg tggacggaca
      661 aaagggcccc tggaagaagc tccaccgcgg ggcaggcgcg ttgggcggtg gcgtgtcctg
      721 tcaggatgcc tcggcgagcc tgaccttact cacgcgagtt tcgtagcctg cgcgatggcc
      781 gcgatagtag tcgaggcgat ccgctgcgct tccccaccgg gcgaggcggc ggttgtccgt
      841 gaacctctgc gggtcaccgt agcggccgtc gagccagccc gaccagtacg cttctgaatg
      901 gtccggggac ttccaggaca ccggggccga gatgggggta acctccgatc cacaggccgg
      961 acatcggtac acatcgtcgg gtaactttct catctcggga tggccgcact cgcaggcgtg
     1021 accgcccgcc gcgtcgggga gcgagatgat cttgttcagc gtctcgagca gggccccgcc
     1081 cagaacgcca ccacattcgt cacacctcaa ggagttgcgc ccgaactcac gaagctcggc
     1141 ctccgagcag aaaggacata ccagggttcg ggacgctttg cttggcttgt ccatgatgcc
     1201 cacctttgct cagtccgctt tataacctaa tcatgaagca cagggcacaa atcaacatcg
     1261 cccaaatggg tgatttctgc gtaacccata ttaggtagaa cctatggact tcggagcagg
     1321 gctcgtgcga aggtctctgg tgggggtgcg ttccgggtta tcgtctcaaa gtccgaagct
     1381 gccgctctcg agctggtcca gcacgcgctg gcgaagggta tccgttcctg gaggcggtga
     1441 gtacatctca ctcaggggaa ccagcacgtc tatggcgccg tccggggtgg gcgacgaggc
     1501 gggaacggtg gcgcgggtgc cgtctggcag gtcgaggagg cgcttgcggc cgccgcgctt
     1561 tccgcgcttg gagcggtctt caccattcac ctccacgagg tcgagcgagt agtcgatcat
     1621 tggggcgtcg gctatggccc cgccgacgcc gtaagcgtcg caggccgggt tcaggtggag
     1681 gatgtagtct acgtcgatgc cgccggaggc gaagatcttc acgtgcccga agccgtttcg
     1741 gtcgagctcc cagcggacct cacgcatcag gtccaggaag tcgcctcgtc tattgccagg
     1801 ggtgtccatg cggatggcgt agacggagtc ggggatggcc cgggcggcta gcagggctcc
     1861 gaacttctcg tcgtcgaagg tgtcgatgat gatcgtgcgc ggcacgtccg gttcgaccac
     1921 ctcgtcgaag gcgcgtgccg cctccgccgt ggaaccaata agcagtacca gggcgtgggg
     1981 ggtcgtcccg gtcgccggga taccgaggcg cttcatggcc agatcgacgg ccaccatatc
     2041 gcatccgccc aggtaggcgg cgcgttcgat catgggcgtt acagccgggt gcatcctgcg
     2101 ggcgccgaac gagatgacgg gcttctggcc ggcggcgagc cggcagcggg cggcccccgt
     2161 tgccacgccc gaggcctggc acaagaggcc gagaagggcc gtctcgtgtt cggcgaaggc
     2221 gccgtaggac cccgagagcg tcaggatcgg ctcgttcggg cggcagatcg tgccttccgg
     2281 taccgcacgc acgtcgacgt cgacttcctc caagaggtag gcgacctcgt cgaggcccgc
     2341 gaccacgaac catgcctcgg gatcgctggt cttgaaggcg atctccgcat gtaccggcgt
     2401 gtcttgcagg ccgcgggccc gcaggatctg catggtcctg tggaagtagg aatcggcgac
     2461 gtcggcgttc aggatctcct tgtccgtcgc cgagttgaac ctcctaccgc cgcctctgtc
     2521 ctcgctcaag atgcgaaccc cttacgctcg agtgactacc gccatatcat agccaatcgg
     2581 caccggagtt gatcccgcgc ggcatgagag ggccgagaac gtgcataata gctggttgtg
     2641 aatagtactg gcctcaatca ttcattcgcc gccgctgctg gcacgcctta cgcggccgag
     2701 gcggcggcgg agacgtaccg tgctgggggc aacgcggcgg acgctgccgt cgccgcggcg
     2761 gcggccgtcg gcgttaccga gccgctgatg agctcgatcg ggggcggcgg cttcgccctc
     2821 gtcagggacc cgtctggcgg ggccgagctg attgactact acgacacgat
//
LOCUS       DBNGVD010000362         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1542015, whole genome shotgun sequence.
ACCESSION   DBNGVD010000362 DBNGVD010000000
VERSION     DBNGVD010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1542015"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..970
                     /locus_tag="AC16CM_09725"
     CDS             113..970
                     /locus_tag="AC16CM_09725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140150.1"
                     /translation="MYRRELREQNRSSWNAAVGAHDSHRANLPGFLLGGGSTLFPEEV
                     GLLGELRGKTLLHLQCNSGGDSVSLAGLGARVTGIDISDEAIASALKLAGQTGARVRF
                     ERADLYDWLREAPREGRRFDVIFASYGVVCWLPDLVAWAGGIAGVLEPGGCFVLVDFH
                     PVADIFDKDGNHARDYPSGGEPVYLQEGVGDYVAESGGGLTPAGYVEGAAGFRNPEGA
                     HLFRWGLGEVVTALAGAGLRITALHEYPYTNGERCLAGMRQLSGRRLVPPENVPAIPL
                     MYGIRTEGD"
     gene            1130..1888
                     /locus_tag="AC16CM_09730"
     CDS             1130..1888
                     /locus_tag="AC16CM_09730"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR3140151.1"
                     /translation="MIDYHLHVIAHGDRPMTVENILEYCEVARARGVRQMGITEHDRY
                     LDEIDLAAFQEARELSRDVELRLGIEIDFVPGAEERMDHDATALPYDYVIGSVHRVDN
                     QEVDRAEDQSIYERYDTYDLYAAYYANVRKAALSGRFEVIGHPDLIKIFRRFPERDIT
                     PILEETAEAVAESGVVVDVNAAGLRKPVREIYPSRDFLRMFHERGVPIVLSSDAHAPD
                     QVALGYDTSLALVHEIGYREVATFRDHERGTLPL"
     gene            1885..2595
                     /locus_tag="AC16CM_09735"
     CDS             1885..2595
                     /locus_tag="AC16CM_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3140152.1"
                     /translation="MTEELFSGDSYIREFESRVARLEGREVILDRTAFYPGGGGQPAD
                     KGAIGIGPVRASVVDVRREGGNVVHVLDTAIPGTVRDLKGTLDWDRRYAHMRYHTALH
                     VLSGVIWRNFEAKVTGGQMRTDRARMDFSFPGEWTADVVAEIERLTNASLAEGRPVKV
                     YELEREEALANPDLIRTQVNLVPERVERIRIVEIEGLDTQADGGTHVANTSEVGEMGI
                     TGHKSKGRQNKRIEFALR"
     gene            2607..>2798
                     /locus_tag="AC16CM_09740"
     CDS             2607..>2798
                     /locus_tag="AC16CM_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140153.1"
                     /translation="MALVVLVVSLAGCGGAPGGQDADSGEKAGSGTPDLSTMTIDASG
                     GEKVEVRVEIADDPLERERG"
BASE COUNT          523 a          857 c          953 g          465 t
ORIGIN      
        1 gatccgacgc gcctccggcc tgccccgatg ggaagacacg gtcgcgggcc tggaagctgt
       61 cctgaagggc atcctgcgta ccgccggagt gggggcgagg tagaatccac ccgtgtaccg
      121 tagagagctt cgcgagcaga accggtcgtc gtggaacgcc gcggtcgggg cccacgacag
      181 ccaccgcgcg aacctccccg gcttcctcct cggcggtggc agcacgctgt tccccgagga
      241 ggtcggcctc ctcggagagc tgcggggcaa gaccctcctc catctccagt gcaactctgg
      301 gggcgattcg gtaagcctcg ccgggcttgg cgcgagggtc accggcatag atatcagcga
      361 tgaggcaata gcctccgccc taaagctcgc cgggcagacc ggcgcccggg tacgtttcga
      421 gcgggccgac ctgtacgact ggctgcggga ggcgcctcgc gaaggccggc gcttcgacgt
      481 catctttgcc tcctacggcg tcgtctgctg gcttcccgac ctcgttgcct gggcgggggg
      541 tatcgcgggc gtactcgaac ccggcggatg cttcgtgctc gtcgacttcc acccggtggc
      601 ggatatcttc gacaaggatg gaaaccatgc cagagattac ccttcaggcg gagaacccgt
      661 gtacctgcaa gagggtgtag gcgactacgt ggccgagtca ggcggcggcc tcaccccggc
      721 cgggtacgtg gaaggagcgg ctggtttccg aaacccggag ggtgcccacc tgttccgttg
      781 ggggctcggg gaggtcgtca cggcgttggc gggggctggc ttgaggatca cggccctgca
      841 cgagtacccg tacacgaacg gcgagagatg cctggccgga atgcgccaac tctcaggcag
      901 acgcctggtg ccaccagaga acgttcccgc cataccactg atgtacggta tcaggaccga
      961 gggcgactag gaaggccaaa ggctgcagga aatgtacccg atgcctttca ctcgaaaaga
     1021 tcgccagggc gccggggttt gtggcgcttc aagcacctac cgcccgaaaa ctcgacctcc
     1081 ttcttataag ataggggggt cattcgcaac cattttcgga gagtagcctt tgatcgacta
     1141 ccacctgcac gtcatagccc acggcgatcg tccgatgacc gtggagaaca tactggaata
     1201 ctgtgaggtt gccagggcgc ggggcgtccg ccagatgggg atcacagagc atgaccgcta
     1261 cctcgacgag atagacctgg ccgcgttcca ggaggctcgt gagctatccc gcgacgtgga
     1321 gctgcgcctg ggtatagaga tagacttcgt accaggcgcc gaggagcgta tggaccacga
     1381 cgccacggcc ctaccgtacg actacgtcat aggcagcgtg cacagggtag ataatcagga
     1441 ggttgacagg gccgaggatc agagtatcta cgagcgttac gacacctacg acctctacgc
     1501 agcctactac gccaacgtgc gcaaggccgc cctctccgga cggttcgagg tcatcggcca
     1561 ccctgacctg atcaagatct tccgtcgctt ccccgagcgg gacatcaccc ccatccttga
     1621 agagactgcc gaggccgtcg ccgagtccgg cgtggtcgtc gatgtgaacg cggctggcct
     1681 gaggaaaccg gtccgcgaga tctatccttc cagggacttt ctgaggatgt ttcacgagag
     1741 gggggtcccc atcgtgctat cctccgacgc gcatgccccc gaccaggtcg cgctcggcta
     1801 cgacacgagc ctcgccctgg tccacgaaat cggctaccgt gaggtcgcca cgttcagaga
     1861 ccacgagagg ggaacgcttc cgctatgacc gaagagctgt tctcgggcga ctcttacatc
     1921 agggagttcg aatcgcgcgt cgcaaggctc gagggccgcg aggtgatact ggaccgcacc
     1981 gctttctatc ccgggggcgg ggggcagccc gctgacaagg gggcgatcgg catcggtccg
     2041 gtgcgggcga gcgtggtgga cgtgcgtcgt gagggtggaa acgtcgttca cgtgctggat
     2101 acggcgatcc cgggtacggt gcgtgatctc aaaggaacgc tggactggga ccggcggtac
     2161 gcgcatatgc gttaccacac ggccctgcac gtgctctccg gcgtgatctg gcgcaacttc
     2221 gaggcgaagg tgacgggcgg ccagatgcgc acggaccgcg ccaggatgga cttctctttc
     2281 cccggtgagt ggaccgccga tgtggttgcc gagatagagc gcctgacgaa cgcgtccctc
     2341 gctgaaggcc gtccggtaaa ggtttacgag ctggagcgtg aggaggcgct tgccaacccc
     2401 gacctgatcc gcacccaggt caacctggtc cccgagcggg tggagcggat acggatcgtg
     2461 gagatagagg gcctcgacac gcaggccgac ggcggcacgc acgtcgccaa caccagcgag
     2521 gtgggcgaga tggggatcac gggccacaag agcaagggcc ggcagaacaa acgcatagag
     2581 ttcgcgctcc gatgaggttc cgatggttgg ccctcgtcgt cctggtcgta tctctggccg
     2641 gatgcggcgg tgcgcctggt gggcaggacg ccgactcggg cgagaaggcg ggttccggca
     2701 cgccggacct ttccacaatg acaatcgacg cctcgggagg agagaaggtc gaggtgcggg
     2761 tagagatcgc cgacgacccc ctggagcggg agcggggc
//
LOCUS       DBNGVD010000363         5398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_267185, whole genome shotgun sequence.
ACCESSION   DBNGVD010000363 DBNGVD010000000
VERSION     DBNGVD010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_267185"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16CM_09745"
     CDS             <1..474
                     /locus_tag="AC16CM_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3140154.1"
                     /translation="RNMPCGEVFTGPIEDSANGTVYFGVPAAIGGREVSGARLRFEGG
                     KVVEASAERGDDFLQSLLDTDPGARFLGELGIGTNYGITQASANVLFDEKLGGTIHLA
                     IGRSYEETGGKNQSSVHTDLVCDLRDGGQLYADGELIQENGRFLEFDLAGTTDDR"
     gene            464..700
                     /locus_tag="AC16CM_09750"
     CDS             464..700
                     /locus_tag="AC16CM_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140155.1"
                     /translation="MTGESMLRVANVGDEAAMESIRDTLDSLDVAYQYIRSDPEEDRF
                     PQTAYFYVPDDTAEGVEQALAALSQEHGFDAEVL"
     gene            960..1643
                     /locus_tag="AC16CM_09755"
     CDS             960..1643
                     /locus_tag="AC16CM_09755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3140156.1"
                     /translation="MRYDCVFLDIDGTLLWVDLDVEGYVEDLAPYSDNGGLSVEGVAG
                     PVWGSLRRHIEENIEYRTAEDLEEFRRRNAMMTAAEIGVEAPVEVLADVARRRISFYP
                     YPESESVLNRLRELGVKLYAVSNWDIELIKVLDDLGWSTYFEEVIASAVVGVEKPAGE
                     IFEEALRASNSPRDRVVHVGNDPVTDVAGASRAGIDSVFVDRKGQAEATQATFVLPDL
                     NDLPAIVEG"
     gene            complement(1665..2423)
                     /gene="ppgK"
                     /locus_tag="AC16CM_09760"
     CDS             complement(1665..2423)
                     /gene="ppgK"
                     /locus_tag="AC16CM_09760"
                     /EC_number="2.7.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0047330 - polyphosphate-glucose
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--glucose phosphotransferase"
                     /protein_id="HIR3140157.1"
                     /translation="MNVFGLDIGGTGIKGAPVDTETGELVQKRVRIVTPQPATPEAIV
                     RTSAEVVSEAGWEGPVGCGFPAVVKDGVLQTAANVDKAAIGFDLRTALSKELDTQVSV
                     VNDADAAGLAEMRWGAGRDHEGVVLMLTLGTGIGTSLFIGGRLVPNTELGHIEVRGKD
                     AEYRASDTARKRVDLNWKHYDKRLDEYLHRIENLLWPDLIIVGGGISKKSEKYFPHLT
                     NRTEVVRAEMQNNAGIAGAGLAGLPQRAAVQTAE"
     gene            2772..3560
                     /locus_tag="AC16CM_09765"
     CDS             2772..3560
                     /locus_tag="AC16CM_09765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140158.1"
                     /translation="MVALRGRVVVITGAGGIGSEIARAFAEEGCRVAICDRVEEGLEG
                     VRERLGGSGGEVFTMVADVSDELQVEGFMEAAVRRWGRVDVLVNNAAYGLHKPFLKTS
                     TDEIQAQLQTNYMGVVYATRAALKHMVPARSGHIVNIVSVVAKLPVPNSGSYAATKAA
                     LDSLSLTLRAELAEYDVLVTTVYPTAVRNTNFFRTSGSSIEDTWARRFMKEPSEIAAA
                     VLASVRRFRPEVHVGVQTRILPVVRALLPPVVRLALRWIARVYQ"
     gene            complement(3712..4191)
                     /locus_tag="AC16CM_09770"
                     /pseudo
     CDS             complement(3712..4191)
                     /locus_tag="AC16CM_09770"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            4404..5198
                     /locus_tag="AC16CM_09775"
     CDS             4404..5198
                     /locus_tag="AC16CM_09775"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3140159.1"
                     /translation="MRRPLQVGWSGRPWRGPIIAGVIMLAVVVVAGFFAFRALGGTEQ
                     LGVLLEQVQTIGLSMRDWIEQSGPLAPFTYLVAKALTFIFLPWAGYPLNVASGALFGL
                     FWGVLLTALGDTLGGCILYGLSRWAGRPAVVRVLGERRMARVDRIVDTGLGGWRELLF
                     FRVVVPIPYNVVSLAAGLAPTLTLRQYATVTFLTAAPKVFTVGIGAGLVTGQWMEVAV
                     AALLVVIAVLAMLTLPSVRKALKRVLQWTRREERIEHSSQGEVRER"
BASE COUNT          982 a         1640 c         1760 g         1016 t
ORIGIN      
        1 cgcaacatgc cttgcggcga ggtgttcacc ggccccatcg aggattctgc caacggaacg
       61 gtatatttcg gcgtgcccgc cgcgatcggg ggccgcgagg tctcaggcgc gaggcttcgc
      121 ttcgaaggcg gcaaggtcgt cgaagccagc gcggagcggg gcgatgattt tctgcagagc
      181 ctgctcgaca ctgacccggg ggcgaggttc ctgggagaac tcggtatcgg caccaactac
      241 ggcatcaccc aggcgagcgc caacgtcctg ttcgacgaga agcttggcgg taccatccac
      301 ctcgccatcg gacgctccta cgaggagacg ggaggcaaga accagtcgag cgtacacacg
      361 gacctcgtct gcgacctgcg tgacggcggg caactctacg cggatgggga gctgatccag
      421 gagaacggac ggtttctgga gttcgacctg gcggggacga cagatgaccg gtgagtcgat
      481 gctgcgggtc gccaacgtgg gcgacgaagc cgcgatggag agcatcaggg acacccttga
      541 tagcctggac gtagcctacc agtacattcg ctccgacccc gaggaggacc gctttcccca
      601 gacggcgtac ttctacgttc ccgacgacac cgcggagggt gtggagcagg cgctggccgc
      661 tctctcgcaa gaacacggct tcgacgccga agtgctgtag aacggagcca acccaccagt
      721 ccccgtttcc ccgtcgttgc ggcgaaagcg ggtacccaag gatagacgaa gagtcgccgg
      781 ccgcgctaca gcatcgcttt cgaggtgggg ccgatcccgg gtggtgtggt tccggttttg
      841 aggtaatgct tacgtttgag ggagtcggcc gaggttatac tcgggctctc accaggtacg
      901 gaaagttgtt cgaggtagcg aggtcctttg gcccatttcg acggaggggg gttgcacggt
      961 tgcgatacga ttgcgttttt ctggatatcg acgggacgct gttgtgggtg gatctggacg
     1021 tggaagggta cgtcgaggat ctggcacctt actcggacaa cggtggactc tccgttgagg
     1081 gtgtcgccgg ccccgtgtgg ggcagcctga ggcgacacat cgaggagaac atcgagtaca
     1141 ggacagcgga agacctggaa gagttcaggc ggcgcaacgc tatgatgacc gcggctgaga
     1201 ttggcgtgga ggcaccggtg gaagtcctgg cggatgtggc gcggcgcagg atatccttct
     1261 acccgtaccc ggaatccgag tcggtgttga accggctcag ggagttgggc gtgaagctgt
     1321 acgcggtctc caattgggac atcgaactca tcaaggtgct cgacgacctg ggatggagca
     1381 cttacttcga agaggtgatc gcctcggccg tcgtcggcgt ggagaagccc gctggcgaga
     1441 tcttcgagga ggcgcttcgc gcgagcaaca gcccacgcga ccgggtagtc cacgtgggca
     1501 acgatcccgt caccgatgtc gcgggcgcct cccgggcggg aatcgattcg gtgttcgtcg
     1561 accgcaaggg ccaggcggag gctacacagg caaccttcgt gctccccgac ctgaacgatc
     1621 tgcccgccat cgtcgaaggg taacggcgga ccggagggcc cacgctattc ggcggtctgc
     1681 accgccgctc gctgggggag gccagccagt cctgcgcctg cgataccagc attgttctgc
     1741 atctcggccc gcaccacctc ggttcggttc gtgaggtggg gaaaatactt ctcggacttc
     1801 ttgcttatgc cgccgcccac gatgatgagg tcgggccaga ggaggttctc gatcctgtgc
     1861 aggtactcat cgaggcgttt gtcgtaatgc ttccagttca ggtcgacgcg cttgcgggct
     1921 gtgtcggaag ccctgtactc ggcatctttg cccctgacct cgatgtgccc cagctcggtg
     1981 ttcggcacca gccggccgcc gataaacagg gaagtgccga tgcccgtgcc aagcgtgagc
     2041 atgagcacga cgccctcatg atccctgcca gcgccccacc gcatctcggc cagccccgcc
     2101 gcatcagcgt cgttgaccac gctcacctgg gtatctagct ctttggaaag ggcggtgcgc
     2161 aggtcgaagc cgatggcggc tttgtccacg ttggccgcgg tctggagcac gccgtccttg
     2221 acgacggccg ggaaaccgca tcccacagga ccttcccagc ccgcctccga gacgacctcg
     2281 gccgaagtac ggactatggc ctccggtgtg gcaggctgcg gggtcactat cctgacccgc
     2341 ttctgcacga gctcgccggt ctccgtgtct accggggcac ccttgatccc cgtcccccca
     2401 atatccaatc cgaacacgtt catgcttcct ccccaggtaa ataacggtca tcattctata
     2461 cgattccctg tttaccctgg gtgccgatac cactctgccc ttgttcgaac ataggattgg
     2521 gtgtgaaggt tcgtgcgccg cgacccgggc gagagattcg atagggttgg tttacgcagg
     2581 gtcagggcat tgaagacatg cgctgatgcc gatccaatgc cccgggttga cctttgatcc
     2641 agatcaattg ttttcgtccc gataggggtg tatcgtgtct gccatgctgt tattgcgaac
     2701 gactctcact acacgaacgc ggtattgaac tctctaatac gaggggctgt gatgcgaggc
     2761 gggtccgcga gatggtagcc ttgcggggca gggtggtggt gatcaccggt gcaggcggca
     2821 tcggttcgga gatcgcccgc gcgttcgctg aagaagggtg ccgcgtggcc atctgtgacc
     2881 gcgtggagga gggcctcgaa ggcgtccgcg agcggctcgg ggggagtggc ggtgaggtct
     2941 tcaccatggt cgctgacgtg tccgatgagt tacaggtcga gggctttatg gaggcggccg
     3001 taaggcgttg ggggcgggtg gacgtgctgg tcaacaacgc cgcctacggt ctacacaagc
     3061 cgttcctgaa gacgagcacg gacgagatac aggcccagct acagaccaat tatatggggg
     3121 tcgtgtacgc taccagggca gccctgaagc atatggtgcc agcccggagc ggccacatag
     3181 tcaacatcgt ttcggtggtt gccaagctcc ccgtgcccaa ctcgggaagc tacgcggcga
     3241 ccaaggcggc gcttgattcg ctctccttga cccttcgggc cgagctcgcg gagtacgacg
     3301 tcctcgtaac gaccgtgtac ccgacagcgg tgcgcaatac caacttcttc cggacctccg
     3361 gctcctcgat cgaagacacc tgggccagac gttttatgaa ggagccatca gagatagcgg
     3421 ccgccgtctt ggcctccgtc aggcgtttcc gacctgaggt gcatgtcgga gtccagacgc
     3481 ggattctccc cgtggtccgg gcgttgctgc cgccggtggt tcggctggcg ctgcgctgga
     3541 tagcccgggt ttaccagtag ccgccgagga cttatcgaac tgcccacctg atgcgggtgc
     3601 gaacaccagc aggcgccgcc ctcctatcat cgccagcaag gccaacagcg cgataccggc
     3661 atacgccatc acgcgtgcct cccagcccag cgtgacgctc gcgccgacgg ttaccaggat
     3721 tcccgtgtga atgatgccgc cgaaggtggt aaccgccaga tactgccgga aggggagcct
     3781 ggtcgagccc gcagcgaagc tcacgagatt gtagatggcg gggaaagtaa ccctgaagaa
     3841 caggaggccc cgccaacctc ccagcccgtc cccgagcagc gcgttcagac gcttcatgcc
     3901 ggcgtcaccc gtgaggcgaa cgagtgatcc ccgccccaga tagcgcgcgg ccacaaagca
     3961 gacacaacca cctgtgacgt cgcctatcaa cgagtagaag aacccacctg ctagcccgaa
     4021 gagcgcgccg gaggccgctt tgagcggaac gccgctgaac ggcgccacga tggtcgcaac
     4081 agccttggcc ccaatatagg tgagcggggc caagggcccg gagctctcta cgagctcccg
     4141 tagccgctcg cccccatcga aagccccgac gagcaagggc aaccctacca cgagcgcgac
     4201 gaccgccaga accctcgcta ctgcgcccca acgcaaggtc cccgatgctc ccgcgtcaga
     4261 tacgtactcc gaagtattcg acggctctcc gaacccccgc gacgccgtag cttgaaaatt
     4321 cctcaaagaa cccctatcgt acaggtcttg atactgattc tcatttgcac gtcaaggata
     4381 catgagagga gcgtggggag caattgaggc gaccattgca ggttgggtgg tctggtagac
     4441 cgtggcgcgg cccgataatc gctggcgtga tcatgctcgc cgtggttgtt gtggcgggct
     4501 ttttcgcttt ccgggcgctc ggcggcaccg agcaactggg ggtgcttctg gagcaggttc
     4561 agaccatcgg gttgagcatg cgggactgga tagagcagtc tggtccactg gccccgttca
     4621 cctatctagt ggcgaaggcg ctaacgttca tcttcctgcc ctgggctgga tacccgctca
     4681 acgtggcatc gggcgcgctc ttcgggctct tctggggggt cctgctcacg gcgctcggtg
     4741 acacgctcgg cggctgcatc ctgtacgggc tgtcgaggtg ggcgggacgg cctgcggtgg
     4801 taagggtcct cggcgagaga cgcatggcaa gggtggatag gatcgtggac accggcctcg
     4861 ggggctggcg cgagttgctg ttcttcaggg tcgttgtgcc cataccctac aacgtggtca
     4921 gccttgccgc cggcctggct cccacgctca ccttgcgcca gtacgcgacc gttacctttc
     4981 tgacggctgc ccccaaagtg ttcaccgtgg ggataggtgc tggcctggtc acgggccagt
     5041 ggatggaggt tgcggtcgcc gccttgctcg tcgtgatcgc cgtgctggcg atgctcacgc
     5101 tgccgagcgt caggaaggca ctcaagcgtg tcttgcagtg gacgcggagg gaggagcgca
     5161 tagagcacag ctctcagggc gaagtgcggg agcgttaggg ccctttttac tctatacgat
     5221 aacgcttcgg tcccaggatg cgttggcggc gaggtctagc cgtctcgtca gcagcatcac
     5281 gggcctggga ccgatccctt cacccccgat aaccgtctac ctgccacgga ctcgtgattg
     5341 cgtaccgaga ttgccggctt cggacgtcga acggtgccgt ggcgttcgac acaaaacc
//
LOCUS       DBNGVD010000364         5334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2088859, whole genome shotgun sequence.
ACCESSION   DBNGVD010000364 DBNGVD010000000
VERSION     DBNGVD010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2088859"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC16CM_09780"
     CDS             complement(<1..323)
                     /locus_tag="AC16CM_09780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140160.1"
                     /translation="MESSFAGLAAGYNASGGTLRRVVRHELVDRGLAEYLPGPPARIV
                     DVGGGAGQHTIPLQRRGYEGTILEPDREMLDEARRRLAAEGPEARRLSRLVEGTGERA
                     QEALGG"
     gene            complement(332..2038)
                     /gene="polX"
                     /locus_tag="AC16CM_09785"
     CDS             complement(332..2038)
                     /gene="polX"
                     /locus_tag="AC16CM_09785"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3140161.1"
                     /translation="MNNNDIVRSLEMIATLMQIKDEAYYRWLGYQRAAESVQALGRPV
                     KEVDDLKKLPHVGATTAEVIRDLAEDRTPQLLTELTEEIPVGLVEMTRLPSVGPRTVG
                     RLWKELGITSVEGLAQEDPARISALKGFGKKSAEKMVQSAENYNATERRMLLDTATDL
                     GDEILSFVLSHPATERAEVGGSLRRMKETIGDIDIVAASTDPASLADDFAEAPFAEEV
                     LAHGPKKVFIISGGTEVDLRIVDPEAYGTLLHHFTGGQAHNISLRERAVKMGINISEY
                     GLAEIGTGDYKPVATEEDLYSRLGLAYIPPELREDTGEIEAAATGNLPDLVSVEDVRG
                     DLHVHTNYSDGKGTIESMAEAAIELGYEYLVFCDHSQSLRVANGLSPERLEQKLAAVR
                     EADEKYGEIKLFCGSEVDILKDGTLDYEDDLLATLDFVVASVHTSFGVGEKAMTDRIV
                     RAIDNPHVRTIGHPTGRILNRREPYEVDVSRLIKEAAATNTALELNSYVDRLDLSVPF
                     VREAVDAGVRITVDTDAHDERALSYMKYGISQARRAWVEKGSVINCLPLAEFERYLMD
                     GK"
     gene            2127..2846
                     /locus_tag="AC16CM_09790"
     CDS             2127..2846
                     /locus_tag="AC16CM_09790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3140162.1"
                     /translation="MLRENREFVRWASERLTVVAAVWLAAGLALSVFVVWAFLELTDE
                     VLEGESRAFDRAVLLWIHDTFPGWLDGPMRLVTALGYYYVVLPLLAVVTLGFYWRGWR
                     LSAVLLVVSTGGSIVLTTILKGVFQRTRPELFDSGYQASFYSFPSGHATVAVGFYGML
                     TVMLAYRSQGAGRWAVATGGVLVVLLIGFSRLYLGVHYPTDILAGYLSALLWLVCVGG
                     VYALWLPVRGLRSAKSGRNAG"
     gene            2859..4667
                     /locus_tag="AC16CM_09795"
     CDS             2859..4667
                     /locus_tag="AC16CM_09795"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3140163.1"
                     /translation="MENILHLIEQYGYILVLFGVMVESMGVPLPGETILICAGILAQR
                     GHLGLGYVILLGVIGAVVGDQLGYWIGREGGRPFILRYGRYVFITPQRLGRAESFFAR
                     HGGGAVFMARFFSGLRVFSALVAGMSRMRWSRFFLYNALGGIVWATVVVLAGYFLGTS
                     LKVVERWTGQASVLLLALAVLAVLMYLAYRWVLRHPERVKMLFDRVGGRHVYAFLESR
                     PGLWLERRFSPNGIYGLALTVGLVLTGLFSWAFGGIVQDVVARDPLVRTDLAVVRYFH
                     AHGEPYLTTCVVVFEAILSPSVLLTAATIAGLALLLLARRRGDFETRLSGIVLLAAAA
                     GTGALSVLFKLLFHRPRPAASLQLVPETGFGFPSSHAMVAVVVGATVWYLFGLRPLGR
                     WGVTWQERSRIGLAVVALALLVGLGRVYTGASFPSDVLAGWALGGVWASVCLTAAEVF
                     RRLRAEGQKLPEAGVRYMRFSLVGVSNALVDLGAINLLLFFHPTREPLLLVLYNLLAL
                     AVTNVNSYLWNTLWTFRHHARHDARQMGLFTLQGVLNAAIGSGVLWVVAHLLLAYYPA
                     LSAQLAGNIAKLASMFVASSASFLFLHYLVFNKKDY"
     gene            4714..5328
                     /locus_tag="AC16CM_09800"
     CDS             4714..5328
                     /locus_tag="AC16CM_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3140164.1"
                     /translation="MLEAILHPLIQFVTTTIGGYGVPAVFVLMLLESMGILIPSEAIS
                     PFAGYLVSEGEMGFLAAVSAGVLGNLVGSWIAYAIGLWGGRELWFRYGRYVGVRAHHL
                     AVAEKWFDRYGELTVFVSRCLPVVRTFISFPAGTARMNFARFTIYTFLGCIPWVFALT
                     YLGYTLGKNWETIGDYLHYLDYAVAVALVGGAVYVLWRWRRSAS"
BASE COUNT          715 a         1784 c         1733 g         1102 t
ORIGIN      
        1 ccgccgagcg cctcttgcgc ccgctcgccc gtgccctcga cgagccgcga cagcctgcgg
       61 gcttcagggc cctccgcggc gagcctccgg cgcgcctcgt cgagcatctc gcggtcgggt
      121 tcgagtatgg tgccctcgta gccccgtcgc tggagcggta tcgtatgctg gcccgcgcca
      181 cctcccacgt ccacgatccg ggccggcggt ccggggaggt actcggcaag cccacggtcg
      241 accagctcgt ggcggaccac ccgccgcagg gtgcctcccg atgcgttgta gcccgcggcg
      301 aggccggcga aggagctttc catgggtagg gctacttccc atccatcaga tacctctcga
      361 actcggccag gggtaggcag ttgatcacgc tccccttctc gacccaggcc cgcctcgcct
      421 gcgagatgcc gtacttcatg taagagaggg ccctctcatc gtgggcgtcg gtgtcgacgg
      481 ttatcctgac gccggcgtcg actgcctccc tcacaaaggg gacggagagg tccaaccggt
      541 ccacgtagga gttgagttcg agggccgtgt tcgtggccgc agcctccttt atgaggcgcg
      601 acacgtctac ctcgtacggc tccctgcggt tcaggatgcg gcccgtggga tgcccgatgg
      661 tgcgcacgtg ggggttgtcg atagcccgca ctatgcggtc cgtcatcgcc ttctcgccca
      721 ccccgaagga ggtgtggacc gaggcgacca cgaagtctag ggtggcaagt agatcgtcct
      781 cgtagtcaag cgtgccgtcc ttgagaatat ctacctctga gccgcagaac agctttatct
      841 ccccgtactt ctcgtcggcc tccctcacgg ccgcgagctt ctgctccagg cgctcgggcg
      901 atagcccgtt ggccaccctg aggctctgcg agtggtcgca gaacaccagg tactcgtagc
      961 cgagctcgat ggccgcctcg gccatgctct cgatggtgcc cttgccgtcg gagtagttgg
     1021 tgtggacgtg aaggtcgccg cggacgtctt ctacagagac gaggtcgggc aggttgcccg
     1081 tcgcggcggc ctcgatctca cccgtgtcct cgcgcaactc cgggggtatg taagcgaggc
     1141 caagccgcga gtagaggtcc tcctcggtgg cgacgggttt gtaatcgccc gtgccgatct
     1201 ccgccaggcc gtactcggag atgtttatgc ccatcttgac ggcccgctcg cgcagcgaga
     1261 tgttgtgggc ctgcccgccc gtgaagtggt gcaacagcgt gccgtaggcc tccgggtcca
     1321 cgatcctgag gtccacctcc gtgccgccgg agatgataaa gaccttcttc gggccgtggg
     1381 ccaggacttc ctccgcgaac ggggcctccg cgaagtcgtc ggctagggag gccgggtccg
     1441 tgctcgcggc cacgatgtct atgtcgccta tggtctcctt catgcggcgc agggagccgc
     1501 ccacctcggc cctctcggtc gccgggtgcg agaggacgaa ggagaggatc tcgtccccca
     1561 ggtcggtggc tgtgtcgagg agcatgcggc gttccgtggc gttgtagttc tcggcggact
     1621 gcaccatctt ctcagcgctc ttcttgccga agcctttgag ggcggagatc ctggccggat
     1681 cctcttgcgc cagcccctcg acgctggtga tgccgagctc tttccacagg cgcccgaccg
     1741 tgcgcggccc cacgcttggg aggcgcgtca tctccacgag gccgacgggg atctcctccg
     1801 tcagctcggt gaggagctgg ggcgtcctgt cttcggcgag gtcacggatc acctcagccg
     1861 tcgtcgcccc gacgtggggt agcttcttga ggtcatcgac ctccttcacg gggcgtccca
     1921 gggcctgcac cgactcggcg gccctctggt agcccagcca gcggtagtag gcctcgtcct
     1981 tgatctgcat gagcgtcgct atcatctcta gcgaccgtac gatgtcgttg ttgttcaagg
     2041 tgcccgctcc tcctgtttcg gggaccatct gtccgccagc aagattatac gcgccgcatg
     2101 gatggctaaa ccgctagaat cgtcccatgc tgagggagaa cagagagttc gtgcggtggg
     2161 cctcggagcg cttgacggtc gtggcggccg tgtggctcgc ggctgggctg gcgctttcgg
     2221 tgttcgttgt ctgggccttc ctcgagctga cggacgaggt tctcgaaggc gagagccgcg
     2281 ccttcgacag ggccgtcctg ctctggatcc atgacacctt ccccggctgg ctcgacgggc
     2341 cgatgcgtct cgttacggcc ctcggctact attacgtggt cctgccgctg ctggccgtgg
     2401 tgaccctcgg gttctactgg aggggctggc ggctatctgc cgtgctactc gttgtctcga
     2461 ccggggggag catagtgctg accaccatcc tgaagggcgt gtttcagcga acgcgtcccg
     2521 agctgttcga ttcgggttac caggcttcct tctactcctt cccgagcggg catgccacgg
     2581 tcgccgtcgg cttctacggc atgctgacgg tgatgctggc ctacaggtca cagggtgcag
     2641 ggcggtgggc cgtggccacc ggtggggtgc tcgtggtgct actgatcggg ttctcccggc
     2701 tctacctggg cgttcactac ccgacggaca tactcgccgg atacctctcg gccctcctgt
     2761 ggcttgtctg cgtcgggggc gtctacgcgt tgtggctccc cgtaaggggc ctgaggtcgg
     2821 caaagtccgg ccgcaacgcc gggtagtatg atcgcctgat ggagaacatc ctccacctca
     2881 tcgagcagta cggatacata cttgtcttgt tcggggtaat ggtagagagc atgggcgtgc
     2941 ccctaccagg ggagaccatc ctgatctgcg ccggtatcct ggcacagcgg gggcatctgg
     3001 gcctgggcta cgtcatcctg cttggcgtaa tcggcgccgt ggtgggggac cagctcgggt
     3061 actggatcgg ccgcgagggg ggccgcccgt tcatactccg ctacgggcgc tacgtcttta
     3121 tcacgccgca gcgtctcggc cgcgccgagt cgttcttcgc ccgtcacggt ggtggggccg
     3181 tctttatggc ccgctttttc tccggcctca gggtcttcag cgcgctggta gccgggatga
     3241 gccgcatgcg ctggtcgagg ttcttcctgt acaacgccct cgggggaatc gtctgggcca
     3301 cggttgtggt gcttgccggc tacttcctcg ggaccagcct caaggtggtg gagcgctgga
     3361 cggggcaggc ctccgtactg ctgctcgcgc ttgcggttct ggctgtcctc atgtacctgg
     3421 cctaccggtg ggtgttgcgg caccctgagc gtgtaaagat gctctttgac cgggtcgggg
     3481 gccgccacgt ctacgctttc ctggagagcc ggccggggct ctggctcgag cgccggttct
     3541 cccccaacgg catctacggc ctcgccctta ccgtgggcct cgtcctgacc ggcctcttct
     3601 cctgggcctt cggcggtatc gtgcaggacg tggtggccag ggaccccctc gtgcgcacgg
     3661 acctggccgt ggtgcgctac ttccacgcgc acggcgagcc ctatctgacc acctgcgtgg
     3721 tcgtgttcga ggccatcctc tctccctcgg tactcctcac cgctgccacc atagccgggc
     3781 ttgcgctcct gctcctggcg cgcaggcgtg gggatttcga gacgcggctc tccgggatcg
     3841 tcttgctggc cgcggccgcc ggcacggggg cgctctccgt cctcttcaag ctgttgtttc
     3901 acaggccgag accagcggcc tcgctccagc tcgtgcctga gacgggcttc ggctttccga
     3961 gctctcacgc catggtggcg gtcgtcgtcg gggcgaccgt ctggtacctc ttcgggctcc
     4021 gtcccctggg gcgatggggc gtaacgtggc aggagaggtc gaggatcggg ctcgccgtcg
     4081 tggcgctggc gctgctggtc gggctgggac gcgtttacac gggggctagc ttcccgagcg
     4141 acgtgctagc cggctgggct cttggcggcg tgtgggcctc cgtctgcctg accgccgccg
     4201 aggttttcag gcgcctgcgc gccgagggcc agaagctgcc agaagcgggg gtaaggtaca
     4261 tgcgcttctc gctcgtgggc gtctcaaacg ccctggtcga tctcggggcc atcaacctcc
     4321 tgctgttctt ccatcccacg cgcgagccct tgcttcttgt cctctataac ctgctcgcgc
     4381 ttgccgtcac caacgtgaac agctacctgt ggaacacgct gtggaccttc aggcaccacg
     4441 cccgccacga cgccaggcag atgggcctct tcaccctgca gggcgtgctg aacgccgcca
     4501 tcggcagcgg cgtgctctgg gtcgtggccc acctgctgct cgcctactac ccggccctct
     4561 ccgcgcagct ggccgggaac atcgcgaagc tagcctccat gttcgtcgcc tctagcgcga
     4621 gcttcctctt cctacactac ctggtcttca acaagaagga ctactgaccc cggcgagcta
     4681 gaattcccaa gcaatggatg gtagcttcac ggcgtgctcg aagcgatcct ccacccgctc
     4741 atacagttcg tcaccacgac catcgggggc tacggcgtcc ccgcggtctt cgtgctcatg
     4801 ctgctggaga gcatgggcat cctgatcccg tccgaagcca tctccccttt cgccggctac
     4861 ctggtctccg agggcgagat gggcttcctc gcggcggtat ccgccggcgt tctgggcaac
     4921 ctggtgggat cgtggatagc ctacgctata ggtctctggg gcgggcgcga actctggttc
     4981 cgctacggca ggtacgtcgg cgtgcgggcc caccacctcg cggtggccga gaagtggttc
     5041 gatcgctacg gggagctcac cgtcttcgtc tcccgctgcc tccccgtggt ccgcaccttc
     5101 atctccttcc ccgcgggaac ggccaggatg aacttcgcca ggttcaccat ctacactttc
     5161 cttggctgca taccctgggt cttcgccctg acctacctcg gctacacgct cggcaagaac
     5221 tgggagacca taggagacta cctccactac ctcgactacg ccgtcgcggt ggcgctggtc
     5281 ggcggcgccg tctacgtgct ctggcgctgg agacgctccg cgtcgtagca cgct
//
LOCUS       DBNGVD010000365         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1542838, whole genome shotgun sequence.
ACCESSION   DBNGVD010000365 DBNGVD010000000
VERSION     DBNGVD010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1542838"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC16CM_09805"
     CDS             <1..807
                     /locus_tag="AC16CM_09805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3140165.1"
                     /translation="RYMLFDSYRRFSSLAPGAEWVDFTEDMYGLRSIKHPFEVERLKL
                     ATELSEAGMRFVVENLEPGMNATDVRHRYAMGVARTAIEDPRYGDYSDHWILPAVGAG
                     VGVGVDSERDAGLTEGDLVKFDCGTTVNGYRGDGGRTFVYGRPKPAAERLFGVLQEAH
                     DRACDEIRPGLEIGEVFRIAQDHITKNGYPRFRRGHYGHSLGIDTFHEEPPYISEHNR
                     RPIEAGMVLAIETPAYTTDAGAIMIEDLVHVLPHGIERLHTMPHGMRAIP"
     gene            921..1712
                     /locus_tag="AC16CM_09810"
     CDS             921..1712
                     /locus_tag="AC16CM_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HIR3140166.1"
                     /translation="MSTLGLAEPEAAWEACRTGAWDRPTAGICKEYVQANLVVLPYAD
                     AFDFLRFCGRSPKPCPLLEATDPGDPEPRLTAAGADLRTDLPLYRVYRGGDLVEERRE
                     IVGLWRDDFVAFLIGCSFSFEHALISAGVPLRHIEEGTNVPMYRTNVACTPAGEFSGP
                     LVVSMRPIPAGLVPLATTVTARVPQVHGAPVHVGSPEDLGIEDLDTPDYGEPVEVREG
                     ELPVFWACGVTPQAVALEAAPEIMITHSPGHMFITSMANEELLAR"
     gene            1842..>2319
                     /locus_tag="AC16CM_09815"
     CDS             1842..>2319
                     /locus_tag="AC16CM_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140167.1"
                     /translation="MVEWFDFATYAFLATYIAVVFFPSDDQTAALLSTFAIFAVSFFT
                     RPLGGLVFGPLGDRIGRQRVLATVIILMSISTALIGILPSYAQIGFVAPLLLLLLRCL
                     QGFSAGGEYGGAATFLAEYAPDRRRGFTVSWLLFSTLIGFLLGSALSTFLSATLSEA"
BASE COUNT          381 a          739 c          775 g          424 t
ORIGIN      
        1 cgctacatgc tcttcgattc ttaccggcgt ttttcttccc tggctcccgg ggcggaatgg
       61 gtggacttca ccgaggacat gtacgggttg aggagcataa agcatccctt cgaggtcgag
      121 cggctcaagc tggcgaccga gctctccgag gccgggatgc gcttcgtggt cgagaacctc
      181 gaacccggta tgaacgcgac cgacgtgcgg caccgatacg cgatgggcgt agccaggacg
      241 gccatcgagg acccccgcta cggggactac tcggaccact ggatcctccc cgccgttggg
      301 gccggggtgg gtgtgggcgt cgactccgag cgcgacgcgg gcctgacgga gggcgacctc
      361 gtcaagttcg attgtggcac cacggtaaac ggctacaggg gtgacggggg ccgcaccttc
      421 gtctacggca ggccgaagcc ggccgccgag cgcctcttcg gtgttctaca ggaggcccac
      481 gacagggcct gcgatgagat caggccaggt ctggagatag gcgaggtctt ccgcatcgcc
      541 caggaccaca tcacgaagaa cggctacccg cggttccgac gcggtcacta cgggcactcg
      601 ctggggatcg acacctttca cgaggagccc ccttacatct cggagcacaa ccggaggccc
      661 atcgaagcgg ggatggtgct cgccatagag acacccgcct ataccaccga cgccggcgcc
      721 ataatgatcg aggatctcgt ccacgtcctg ccccacggca tcgaacgcct gcatacgatg
      781 ccccacggta tgcgggccat cccgtgagcg gcccgtgcgg ggcttttgcc gctgccttcg
      841 tatgctgcta aggttacccg tgtgggagga tttacgaggg gagaatcgaa ctacgagggc
      901 cctttcttga gggggagctc ttgagcaccc tcggcctcgc cgaaccggaa gccgcctggg
      961 aagcgtgccg cacgggggcc tgggatcgtc ctaccgccgg tatctgcaag gagtacgtgc
     1021 aggcgaacct ggtcgtactg ccctacgcag acgccttcga cttcctgcgc ttctgtgggc
     1081 gcagcccgaa gccgtgtccc ctgctcgagg ccaccgaccc gggagacccg gagccgcggc
     1141 tcaccgcggc cggcgccgac ctgaggaccg acctacccct ctacagggtc taccgcgggg
     1201 gagatctcgt cgaggaacgc cgggagatcg ttggtttgtg gcgggatgat ttcgtggcgt
     1261 tcctgatcgg gtgcagcttc tccttcgaac atgcgctgat ttctgcgggc gtgccgctgc
     1321 gacatatcga ggaaggcacc aacgtgccca tgtaccgcac caacgtggcg tgtacccccg
     1381 ccggagagtt ctccgggccg ctcgtggtct ccatgcgtcc cataccggcc gggctcgtcc
     1441 cgctggcgac gacggtgacg gcgcgggtac cgcaggtgca cggggcgccc gtccatgtcg
     1501 gctcgccgga agacctggga atagaagacc ttgacacacc ggattacgga gagccggtcg
     1561 aggtaaggga aggagagttg cctgtgttct gggcgtgcgg ggtaacgccg caggcggtcg
     1621 ccctggaggc ggcccctgag atcatgatca cgcactcccc cggccacatg ttcatcacta
     1681 gcatggccaa cgaggagctg ctggcccggt agagaagcga cggggagtcc gattacgggg
     1741 aggatgttgt ggaaggtcgc gatgggagcg tagaagcaac cgtaccgcaa gtggaccagc
     1801 aaacggtacg caaggccgtg gtaggggccg cggtgggcaa catggtggag tggttcgact
     1861 tcgccacgta cgcctttctg gcaacgtata tagccgtcgt gttcttcccc tcggacgacc
     1921 agaccgcggc gctcctgagc acgtttgcga tcttcgccgt ctccttcttc acccgcccac
     1981 tgggcggcct ggtcttcggt cctctggggg acaggatcgg gcgccagcgc gtgctcgcta
     2041 cagttatcat cctcatgtcc atctcgaccg ccctgatcgg catccttcca agctacgcgc
     2101 agataggatt cgtggcgccg ctgctcctgc tcttgctgcg gtgcctccag ggtttctccg
     2161 cgggcggcga gtacgggggc gcggcgacgt tcctggcgga gtacgctccc gaccggcgcc
     2221 gcggtttcac ggtgagctgg ctgctgttct cgacgttgat aggcttcctg cttggctcgg
     2281 cgctttcgac gtttctgtcg gccaccctct ccgaggcgg
//
LOCUS       DBNGVD010000366         7399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4456987, whole genome shotgun sequence.
ACCESSION   DBNGVD010000366 DBNGVD010000000
VERSION     DBNGVD010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4456987"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC16CM_09820"
     CDS             complement(<1..377)
                     /locus_tag="AC16CM_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3140168.1"
                     /translation="MAVATTFIVLLITYLNFPVKGVTVEGARMYPESQVTNEVPDNAS
                     LLTLNKNLLERKVESHLWVESAEVNENWNSGIVTVQVKERRPMLYAEVDGREIILSAD
                     GKELPGLGGADLDRMELDRDQVG"
     gene            complement(413..1777)
                     /gene="murC"
                     /locus_tag="AC16CM_09825"
     CDS             complement(413..1777)
                     /gene="murC"
                     /locus_tag="AC16CM_09825"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3140169.1"
                     /translation="MKIHMIGIGGAGMSGIAEVLASRGHEVTGSDLKESPYTRRLREA
                     GITVHIGHTPRQVGDAEQVVISTAIPRTNPELLEARRRSIPVIPRAAALAWILEGNRS
                     VAVAGTHGKTTTTSMTAHVLKALGENPTALVGGELNDIGSNVAFGRSDLVVAEADESD
                     RSLLHLTPQAAVVTNIEFDHPDFYSSLEDVVETFGQFVGALPEYGALITCADDPCCLK
                     VAGDAPCRVMTYGIDSGELRAEILGPNRYALIEDGERRGEVELGVYGQHNALNSLAAA
                     GVARWLGFDAYEAALTLGTFGGVRRRFQLKGEVDDIRVVDDYAHHPTELAATLAAARS
                     TMTNGGRVIAVFQPHRYSRTRALYREFGDSFAAADAVLITEVYGAGEMPQPGVNGKLV
                     ADAICETRGHPEVYYIPQQDAIPRVLGEISERGDLVLTLGAGDISQVGEALLEVLEES
                     GSQG"
     gene            complement(1774..2778)
                     /locus_tag="AC16CM_09830"
     CDS             complement(1774..2778)
                     /locus_tag="AC16CM_09830"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR3140170.1"
                     /translation="MAAELRARDVTVEFVGSTSGIEQTLVPREGYTLHALPLQGFAGN
                     PLKQARAALLSLQAILDCRRIIQKLKPTSVLGVGGYASAPAVVAARALGVPTFLHEQN
                     SVPGRLNRLASRFTLETLVTFPSAGESLGRSVWVGMPTRASIFGASREEALRELDLEP
                     PVVVIIGGSGGALRINLAAAEAFGDTTPYSVVQVSGRRDFPRLSTENPRHRILEYVDD
                     IGPYLAAADVVVSRAGAGSLFDVAAAGKAAILIPFPYATGDHQLHNAAYFIERGAAEL
                     MPDAGVDAVSLRRRVEDLLRDGERREQMAQQIKSLATPHAAGEVADKLLRARGKEQGP
                     "
     gene            complement(2768..3913)
                     /locus_tag="AC16CM_09835"
     CDS             complement(2768..3913)
                     /locus_tag="AC16CM_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3140171.1"
                     /translation="MSSLRANLFLVALGLSMLGLVMVYSATFREVGTHYLFVRATHIV
                     VGVAVFFVVSRVRYTAWRRYAPALYVLVLVSLILVLIPGIGVQINGARRWINLGPMDP
                     QPAEFAKVAAVIVLSCAVARLPAGSGLPLKPLAMVAALFGLVLIEPDFGTSVILLSGV
                     AGVLWASEIKTNVLVLTGLIGLVAVAAMMLLEPYRRERFMMFLDPWAASDGSGYQVVQ
                     GMVAIKAGGFFGAGAGSGGEGPYVPEIGTDMIFALIGEELGLIGMVAVIAAFGFIAVA
                     GLKIALNAPSVMARCVAAGLTTMLTVQAIFNMGAAMVVLPLAGITLPFVSYGGSSLLV
                     CFAAVGILYRISEDSERAREIKPRAAKNPDSRRRHGRPRHPRAVRGG"
     gene            complement(3910..5238)
                     /gene="murD"
                     /locus_tag="AC16CM_09840"
     CDS             complement(3910..5238)
                     /gene="murD"
                     /locus_tag="AC16CM_09840"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3140172.1"
                     /translation="MKTLVFGLGESGVAATRALVERGCAVVVADANDNERLRDTLSGL
                     DVPGTLGAGPEVLEGVDRVVTSPGISPRNPVLRSAEERSLPVVSELGLGLELLGGGTR
                     VAAVTGTNGKTTVVDMIHRMLEAAGEQHAVAGNSWRPLTGCVEEARMAGLLVLEASSF
                     QLHYLKSPGFAVAALLNVRPDHLNWHDSFEEYVADKLRIFDGQQEQNLALLSAEDPVG
                     RGAADDLDAEVLMVGRGGTILRDDSLFLRGEHLADVGELRFVGRHNYEDALFAAAVVQ
                     KLGATPDHIRKALLAYEARPHRVQVVAEVAGVTYVDDSKATNPAAVVAALDSFDAPVV
                     LILGGSEKFTDFSELLPALDGCRAVVLQGEAGPRIAAYLQDEGWGDVVHEAPDLEAAV
                     QRAEALAKSGDVVLLSPGCASFDQFSGYVERGEVFARVVREHAARGTVRR"
     gene            complement(5243..6238)
                     /gene="mraY"
                     /locus_tag="AC16CM_09845"
     CDS             complement(5243..6238)
                     /gene="mraY"
                     /locus_tag="AC16CM_09845"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3140173.1"
                     /translation="MFSVVLGAAVAFLTTVLLGSKFIEFLQSRKFGQFVREEGPQTHL
                     IKAGTPTMGGIVMLTGFFAALVVVARPNVATLTTLLLVVVVAGIGLYDDWQKISKKRP
                     EGLSARYKFLLLTLAVVLADVLAVRYVGVTQTVLVPYFDQSLVLGPGMVGIALFSVFL
                     LLVIAGTTNAVNLTDGLDGLAAGTGAMALLSYTAIAFLERQYDVAVICGAMVGAIVGF
                     LWYNARPAQIFMGDTGSLAIGGVLAAAAVLTKTELLLPIIGGLFVVVAVSVILQVFVY
                     KLTGRRVFKMAPIHHHFELLGWDENKIVVRFWIAQAAFSSVGFFLYYIFLYNSAG"
     gene            complement(6231..>7399)
                     /locus_tag="AC16CM_09850"
     CDS             complement(6231..>7399)
                     /locus_tag="AC16CM_09850"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3140174.1"
                     /translation="RPLDVPTLVVDDPEKALQRLARWTLVRPDAASPTVVGITGTVGK
                     TTTKDALATILRHAGMRVSATEGNLNNELGLPLTVLSADPRTEVLVLEMGATHAGDIQ
                     HLCSIAPPEVGILTAISPVHLDSFGSLEGLAAAKGELALALPESGAFIAPAGAPGRAT
                     GSGRELGRRITFARESEADLVASGVEGHEEGLRFIATLGGESAEVRSPVFGTHLVNPL
                     LAAIGGALALGLDLGVAAEGLARLRRTGLRGDVYRLREDIVVYDDSYNASPAAVAAVL
                     KYGAEQAGGQGRRLVAVLGGMFELGACAREYHREAGRLAEEVGVDLLVCVGDEARWYA
                     EAFSGKTLFYEDAATAANTLSNHLRAGDYLIVKGSRSVGLDTLTHKLRESLALV"
BASE COUNT         1274 a         2490 c         2400 g         1235 t
ORIGIN      
        1 tcccctacct ggtcgcgatc caactccatc ctatcgaggt cggctccccc gagccccggt
       61 agctccttgc cgtcggccga caggatgatc tcccgcccgt ccacctccgc atacagcatc
      121 ggccgccgtt ctttaacctg aacggtaact ataccggagt tccagttctc gttcacctct
      181 gcactctcaa cccaaaggtg agactccact tttctctcca gcagattctt gttcagggtg
      241 agcaggctcg cgttgtccgg aacttcgttc gtgacctgcg attccgggta catacgtgcg
      301 ccttcgaccg tgacgccctt gaccgggaag ttgaggtagg taatcaggag gacgataaag
      361 gtagtagcga cagccacggc cgccgagatc aggatcgcgc gcagtgggtt ctttagccct
      421 gggatccgga ctcctccagt acttcgagca gcgcttcgcc tacctgagag atgtcccccg
      481 cgccgagcgt aagcacgagg tcaccgcgct cggagatctc gccgagcacc ctggggatgg
      541 cgtcctgctg gggtatgtag tagacctcgg gatgtcccct ggtctcgcag atggcgtcgg
      601 ccacgagctt gccgttgacc cctggttggg gcatctcacc cgcaccgtag acctcggtta
      661 tgaggacggc gtctgccgcc gcgaacgagt cgccgaactc cctgtagagc gctcgggtgc
      721 gcgagtagcg gtgtggctgg aacacggcga tcacacgccc cccgtttgtc atggtagacc
      781 gggccgcggc gagcgtggcc gccagctccg tggggtgatg ggcgtaatcg tctacgaccc
      841 ttatatcgtc gacctccccc ttgagctgga agcgtcggcg taccccgccg aaggtcccaa
      901 gggtgagggc tgcctcgtag gcgtcgaagc cgagccatcg cgcaacgccg gccgcggcaa
      961 gcgaattgag ggcgttgtgc tggccgtaga cgccgagttc gacctcgcca cgccgctccc
     1021 cgtcttcgat gagcgcgtag cggttggggc cgaggatctc cgccctcagc tcccctgagt
     1081 cgatgccgta ggtcatcacc ctgcaagggg cgtccccggc tactttcagg cagcagggat
     1141 cgtccgcgca cgtgatgagc gccccatact cagggagcgc gccgacgaac tgtccgaacg
     1201 tctcgaccac atcctccaga gaggaataga agtcggggtg gtcgaactcg atgttggtga
     1261 cgaccgcggc ctgcggcgtg aggtggagga gcgaccggtc gctctcgtca gcctcggcca
     1321 ccaccaggtc ggacctgccg aaggccacgt tgctcccaat atcgttcagt tcgccgccga
     1381 ccagcgccgt cgggttctcc ccgagggcct tcagaacgtg cgccgtcatg ctggtcgtgg
     1441 tcgtcttgcc gtgcgtgccg gccacggcta cgctacggtt gccctccagg atccaggcta
     1501 gcgccgccgc cctcggtatc acgggtatgg aacgccggcg ggcctcgagt agctccgggt
     1561 tggttcttgg tatggcggtg gagatgacga cctgctcagc gtcgcccacc tgcctagggg
     1621 tgtggccgat atggacggtg atgccggctt cacgcagcct gcgggtgtac ggggactctt
     1681 tgaggtcgga tcccgtcacc tcgtgtcccc tgctcgcgag gacctccgcg atgccgctca
     1741 tcccggcccc tccgatgccg atcatgtgta tcttcacggg ccttgctcct tgcctctagc
     1801 acgcaatagc ttgtctgcca cctcgcctgc cgcgtggggc gtggcgaggc tctttatctg
     1861 ttgcgccatc tgctcacggc gctcaccgtc cctgagcaaa tcctccacgc ggcggcgcag
     1921 cgaaacggcg tcgacccccg cgtcaggcat tagctccgcc gcccccctct cgatgaagta
     1981 cgcggcgttg tggagctggt gatctcccgt ggcgtacggg aaggggatga gtatagcggc
     2041 cttaccggct gccgcaacgt cgaacagcga tcccgcgcca gccctgctca ccaccacatc
     2101 ggccgcggcc aggtagggac ctatgtcgtc cacgtactcc aggatccggt ggcgtgggtt
     2161 ttccgtcgag agccgcggga agtcccttct gccggagacc tgcacgaccg agtaaggtgt
     2221 cgtatcaccg aacgcctcgg ccgcggcgag gttgatcctg agcgcacccc cactcccccc
     2281 gatgatgacc accacagggg gctcgaggtc cagctcccgg agcgcctcct cccgcgaggc
     2341 gccgaagatg gaggcccgtg tcggcatccc aacccacacc gaccgcccga ggctctcccc
     2401 ggccgaaggg aacgtcacca gcgtctccag ggtaaaccgg ctagccagcc gattcagacg
     2461 gcccgggacg gagttctgct cgtgcagaaa cgtcgggacg ccgagggccc tggcggccac
     2521 cacggcaggc gcgctcgcat acccgccaac ccccagtacc gaggttggct tcagtttttg
     2581 tattatgcgt ctacagtcga ggatggcctg cagagagagg agggcggcgc gggcctgttt
     2641 cagggggttt ccggcgaagc cttgcagggg gagggcgtgc agggtgtagc cttcgcgggg
     2701 gacgagcgtc tgttcgatgc cggacgtgga gcccacgaac tcgacggtga cgtctcgggc
     2761 gcgcaactca gccgccacgc acagcgcggg gatggcgtgg cctcccgtgc cgcctcctgc
     2821 tatcaggatt ctttgccgcc cgtggtttga tctctctcgc tcgttcactg tcctccgata
     2881 ttctgtagag gatgcccacg gcggcgaagc ataccagcag gctggaccct ccgtagctca
     2941 cgaaggggag cgtgatgccg gcgaggggta gcactaccat ggcggccccc atgttgaaga
     3001 tagcctgcac ggttagcatc gtggtaaggc cggcggccac gcatcgggcc atcaccgacg
     3061 gtgcgttcag ggcgatcttc aggccggcca ccgcgatgaa cccgaaggcg gcgatcaccg
     3121 cgaccattcc gatcaggccc aactcttctc cgatcagggc gaagatcatg tccgtcccga
     3181 tctccggtac gtacggcccc tctcccccgg aaccggcacc ggccccgaag aacccgcccg
     3241 ccttgatggc gaccatcccc tggaccacct ggtagccgga gccgtccgag gcggcccagg
     3301 ggtcgaggaa catcatgaac cgctctcgcc ggtagggctc gagcagcatc atcgccgcca
     3361 ccgcgaccag gccgatgagg ccagtgagga ccagaacgtt ggtcttgatc tcggaggccc
     3421 acagtacacc ggcgacccct gagagcagga tcaccgacgt tccgaagtcg ggttcgatca
     3481 ggacgagccc gaacaacgcg gcgaccatcg ccaggggctt gaggggtagc ccggatccgg
     3541 ccggtagccg ggctaccgcg cagctcaaaa cgatgacggc ggcaaccttg gcgaactccg
     3601 ccggttgggg gtccatgggc ccaaggttga tccacctgcg ggcgccgttg atctggactc
     3661 ctatgcctgg gatgagcacg aggatgaggc tgacgaggac cagcacgtag agggctggtg
     3721 cgtacctgcg ccacgcggtg tagcgcacgc gactaacgac aaagaagacg gccaccccga
     3781 ctactatgtg ggtagcgcgc acgaacagat agtgcgtccc cacttccctg aacgtggccg
     3841 aatagaccat cacgagaccg agcatggaga gcccgagggc gaccaggaac aggttggccc
     3901 gcagactgct catcgtctga cggtcccccg tgcagcgtgc tcgcgcacga ccctggcgaa
     3961 cacttccccc ctctcaacgt acccagagaa ctggtcgaag ctggcacacc cgggcgagag
     4021 caggaccaca tcccctgact tcgccagcgc ctcggccctc tgaaccgccg cttcgaggtc
     4081 gggagcctcg tggaccacgt cgccccaccc ttcgtcctga aggtaggccg cgatgcgcgg
     4141 gccggcctca ccctggagga caacggccct gcatccgtcc agcgccggca gcaactccga
     4201 gaaatccgtg aacttctccg atccgcccag gatgaggacc acgggcgcgt cgaagctatc
     4261 cagggcggcc acgaccgccg cggggttggt cgccttggag tcgtccacgt aggtgacgcc
     4321 tgcgacctcg gcgacgacct gcacgcggtg gggtctggcc tcgtaggcga gcaacgcctt
     4381 cctgatgtgg tcaggcgtcg cgccaagctt ctgcaccacc gccgccgcga acagggcgtc
     4441 ctcgtagttg tgccttccca cgaagcgcag ctctccgacg tcggccaggt gctcgccacg
     4501 caagaagagg ctgtcgtcgc gcaggatggt cccgccacgg ccgaccatca gcacctcggc
     4561 atcgaggtca tccgcggctc cgcgaccaac cggatcttcg gcgctcagga gggccaggtt
     4621 ctgctcttgc tgcccgtcga agatgcggag cttgtccgct acgtactcct cgaaagagtc
     4681 gtgccaattc aggtgatccg gcctcacgtt tagtagcgcc gcgaccgcga agccggggct
     4741 cttcaggtag tggagttgga aggaggaggc ctcgagaacg agaagccccg ccatgcgcgc
     4801 ctcttcgacg cagccggtga gggggcgcca ggagttgccc gccacggcgt gctgctctcc
     4861 agcggcctcg agcatacggt ggatcatgtc aaccacggtg gtcttgccgt tggtgcccgt
     4921 aactgccgcc acgcgcgtac ctccgccgag aagctcaagg ccgagcccta gctcggagac
     4981 gacaggtagg ctccgttctt ccgccgaccg aagcacgggg ttgcgggggc ttatgcccgg
     5041 gctcgtcacg acccggtcca cgccttcgag gacttcgggg ccggcgccga gcgtgcctgg
     5101 cacgtccagc cctgacaggg tgtcgcgcag gcgttcgttg tcgttggcgt cggctacgac
     5161 gacggcgcag ccccgttcca ccagcgcccg cgtcgccgct acgcccgatt ctccgagccc
     5221 gaataccagc gtcttcacgc gctcaccctg ccgagttgta gaggaagatg tagtagagga
     5281 agaagccaac cgaggagaag gcggcctggg cgatccagaa gcggaccacg atcttgttct
     5341 cgtcccagcc cagcaactcg aagtggtggt gtatcggggc catcttgaag accctgcggc
     5401 ccgtgagctt gtagacgaag acctgcagga taacggagac ggccaccacc acgaaaagcc
     5461 ccccgataat tgggagaagc agttcggtct tggtcaggac cgccgccgcg gcgagcacgc
     5521 cgcctatcgc cagcgatccc gtgtcaccca taaagatctg ggccggtcgg gcgttgtacc
     5581 agagaaaccc cacgatggcc cccaccatgg cgccgcagat caccgccacg tcgtactggc
     5641 gctcgaggaa ggcgatcgcc gtgtaggaga ggagcgccat ggccccggtc cccgcggcga
     5701 ggccgtcgag gccgtctgta aggttcaccg cgttggttgt gccggcgata accaggagca
     5761 ggaagacgct gaagagcgcg atccccacca tccccggccc caacacgagg ctctggtcga
     5821 agtagggcac gagtacggtc tgcgtaacgc ctacgtatcg tacggcgagc acgtctgcca
     5881 gaactacggc cagcgtgagc agcaagaact tgtagcgggc gctcaagccc tcgggccgct
     5941 tcttggagat cttctgccag tcgtcataca ggcctatgcc ggcgacgacc acgacgagca
     6001 agagcgtggt gagcgtggcg acgttgggac gggctacgac cacgagcgcc gcgaagaagc
     6061 cggtgagcat gacgatgccg cccatcgtcg gcgtgccggc tttgatgagg tgtgtctgcg
     6121 ggccctcctc gcgcacgaac tgcccgaact tgcgcgactg caagaactcg atgaacttcg
     6181 agccgagcaa gaccgtcgtg aggaaggcca cggccgcgcc gagaacaacg ctaaacaaga
     6241 gctagactct ccctcagctt gtgggtcagg gtatcgagcc cgacactgcg tgaacccttg
     6301 acgatcaggt agtccccggc cctgagatgg ttgctcaggg tgtttgctgc ggttgctgcg
     6361 tcttcataaa agagggtctt acccgaaaag gcctcggcgt accagcgggc ctcgtccccg
     6421 acgcagacga gcaggtccac gccaacctct tcggccagcc tgccggcctc acgatggtac
     6481 tcgcgggcgc acgccccgag ttcgaacatg ccgccgagca ccgccacgag gcgtctcccc
     6541 tgcccgccag cctgttccgc gccgtacttg agaacggcgg cgacggcggc gggggaggca
     6601 ttgtaggagt cgtcatatac cacgatgtcc tctctcaacc tgtagacgtc gccgcggagg
     6661 cccgtgcggc gtagccgcgc gagcccctcg gcggccacac cgaggtcgag tccaagggcg
     6721 agcgcgcccc cgatggcggc gagcaggggg ttcacgaggt gggtaccgaa gaccggggac
     6781 cttacctctg ccgactcacc gccgagcgtg gcaatgaagc gcaggccctc ctcgtggccc
     6841 tcgaccccgg aagccacgag gtcagcctcg ctctcccggg cgaaggttat acgccggccc
     6901 agctcgcgcc ccgacccggt cgccctcccg ggcgcgccgg cgggggcgat aaaggcccct
     6961 gactctggaa gggcgagggc cagctcgccc tttgcggcgg ccaggccttc gagcgacccg
     7021 aaggagtcga ggtgcaccgg cgagatggcg gtgagtatgc cgacctctgg cggcgcgatg
     7081 gagcacaggt gctgtatgtc gccagcgtgc gtggcgccca tctcaaggac gagcacctcg
     7141 gtgcgcgggt cagcggagag cacggtaagg ggtagcccca gctcgttgtt caggttgccc
     7201 tcggtcgccg agacgcgcat cccggcgtgc ctgaggatgg tcgcgagggc gtccttggtg
     7261 gtcgtcttcc cgaccgttcc cgtgatgcct accacggttg gcgaggcggc gtcggggcgg
     7321 accagcgtcc accttgcgag cctctgtagc gccttctcgg ggtcgtccac taccagcgtc
     7381 ggcacgtcca gggggcggg
//
LOCUS       DBNGVD010000367        12376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2089753, whole genome shotgun sequence.
ACCESSION   DBNGVD010000367 DBNGVD010000000
VERSION     DBNGVD010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2089753"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16CM_09855"
     CDS             <1..377
                     /locus_tag="AC16CM_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140175.1"
                     /translation="EQIRSPVVVMAPDLQSARIPAYSDEANVVSRRGSLVLRVLPELE
                     ERVPGEIEVPQGSLDVKSFFYNSTLEKRMEILRRNEVDYVMIPNDSPLVETLERLPGM
                     SRVDTPSERYVLLAVDRSKLPG"
     gene            459..1136
                     /gene="rpe"
                     /locus_tag="AC16CM_09860"
     CDS             459..1136
                     /gene="rpe"
                     /locus_tag="AC16CM_09860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3140176.1"
                     /translation="MTYFGELGDRVVGAPSILAADFAHLAEEVRRAQRGGADLVHFDV
                     MDNHYVPNLTVGPIVAASLAAAIDLPIDAHLQMRDPDNLIPSFIDAGVASIQVQPEAV
                     THLHRTLELIRSGGCEAGLALNPTTPPELIEWALPAVDYINVMSVNPGFGGQDFIPEM
                     VEKVRRVKEMTRVPLLVDGGITEETAPLMVEAGAQVLIAGSAVFNGDPATAMRKIIEA
                     GRSGRSG"
     regulatory      1517..1682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1684..2808
                     /gene="ribD"
                     /locus_tag="AC16CM_09865"
     CDS             1684..2808
                     /gene="ribD"
                     /locus_tag="AC16CM_09865"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR3140177.1"
                     /translation="MLRDSMMGLARCLAERGRYTVAPNPLVGAVIALDGKVVGEGWHR
                     RTGEPHAEVAALAEAGVSAHGATMYVTLEPCNHFGRTPPCVESILRAGISRVVVGHLD
                     PNPRMRGRSVALLREAGVEVEVLDDLAFDRQNEQFFHHMSTGRPFVHLKLAATLDGRI
                     AAAGGDSKWVTGELARQRAHELRAEAGAVLVGANTARTDDPMLTARDLPAPPPSITRV
                     VLDPSLTISPESRLANTADVAPVVVFAAETALDGLQKRLEDRGVEVVAAPDDEGNLDL
                     GRVLENLGGRGIKGLLVEGGGETATRFVRKGLADKMTLFYAPRLLGSEGVPMIGALRA
                     TKVAESLRFSVSDVEKIGDDVAVTLYPDLAGEEDRVHRAC"
     gene            2846..3403
                     /locus_tag="AC16CM_09870"
     CDS             2846..3403
                     /locus_tag="AC16CM_09870"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3140178.1"
                     /translation="MLRVVISADRVTGDTRAGDSISVNGACLTVGESDGEMLAFFAMP
                     ETLRRTALRTLVVGSPVNLERAMAAGSRFGGHIVQGHVDGVGEVLGVRPEGEAEIWEF
                     GAPDSVLRYCLEKGSICVDGISLTIVSVGKDSFTVSILPQTRANTNLGALGQGSRVNL
                     EADVVGKYVERLLEPRMTESFSRGA"
     gene            3407..4609
                     /locus_tag="AC16CM_09875"
     CDS             3407..4609
                     /locus_tag="AC16CM_09875"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3140179.1"
                     /translation="MPFSPIEEILEDIKQGKMVIVCDDEDRENEGDLTMAAELVTAND
                     INFMAAHGRGLICLPMAGEIVDRLDIPEMVTHNASRMGTAFTASIEAREGITTGISAA
                     DRAHTCRVAVDDATGPEDLAMPGHVFPLRAKPGGVLQRAGQTEAAVDLARLAGMTPAG
                     VICEIMKEDGTMARVPDLEKFSAEHDVKLVTVAQIIEYRHAYETQVSCAVETKLPTPF
                     GEFRLRAYENEIDELTHVALVMGEPEGKDDVLVRVHSACLTGDALHSLRCDCGEQLES
                     AMRIIAEEGEGVIAYMQQEGRGIGLLNKMKAYHLQDEGMDTVEANQKLGLAPDLRDYG
                     VGAQILKDLDLRRIRLLTNNLTKVVGLRGFGLEITDRVPLEIEPNGYNERYLKTKREK
                     LNHVFEKF"
     gene            4685..5167
                     /gene="ribH"
                     /locus_tag="AC16CM_09880"
     CDS             4685..5167
                     /gene="ribH"
                     /locus_tag="AC16CM_09880"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3140180.1"
                     /translation="MEGVNYIEGHLTAGGKSFGIVASRFNDFIVKALLDGALDAIRRH
                     GGDTGAIDVAWVPGSYEIPIAAREMALSGRYDAVICLGAVIRGATAHFDYVAGGAANG
                     IASIALETGTPIIFGVITTETIEQAIERAGTKAGNKGSEAAVSAIEMANLMPQVRKGP
                     "
     gene            complement(5289..5489)
                     /gene="rpmB"
                     /locus_tag="AC16CM_09885"
     CDS             complement(5289..5489)
                     /gene="rpmB"
                     /locus_tag="AC16CM_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3140181.1"
                     /translation="MAKVCYSCGKRPDSGNARSHSLRATRRRRNPNLQKVRIQEGPST
                     RREWICTSCLKAFKVQKALASA"
     gene            5677..7263
                     /locus_tag="AC16CM_09890"
     CDS             5677..7263
                     /locus_tag="AC16CM_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR3140182.1"
                     /translation="MSVEVSLLVAAAHAAVKAQVARINDLNVYPVPDGDTGTNMLLTL
                     ESALEETRGEAYASSEEASKAVARAALMGARGNSGVILSQMIRGAAEALTDRSSLDAE
                     TFAAGLEGARGRAYSSVREPVEGTMLTVIKDAAVAAREVIDGGEDDLPTTVEAARRAA
                     HASVRRTPELLPVLREAGVVDAGGLGVAVVLDGVYACVTGEEIEVPEEVDENDTPDLD
                     AIHAQEEAWGYCTEFLVDGFSGDAEEFGEYIYASGRSVFVVADDDVVKVHLHTQDPGE
                     ALTYAGRFGRLAGVKVDDMEAQVRSREKEIPRRPPANLGVVAASRGEGNRRLFEQMGA
                     VVIEGGQGENPSAADLARAVEETGAPVVVLLPNNKNIVPTAEQVGELVGVGTHVVPTT
                     SIAAGLAVMVGYDAEGEPDGVVEEMLEIYSSLRAAEVTISVRDARIGDREVPEGAFIG
                     FLAGELIAVEKTLAGAVIALADKIVGEGADFLTLLKGEDLSEDDLEAMLQAIHELDED
                     LEVEVRDGGQPLYPVQMVAE"
     gene            7260..8075
                     /locus_tag="AC16CM_09895"
     CDS             7260..8075
                     /locus_tag="AC16CM_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3140183.1"
                     /translation="MTTAVVTDSTTSLAPGVLERPDVRMVPLTFNFGSDETYRDKLDM
                     SNAEFYERLRSSDEFPTTAQPSPGAFVQTYEALSDYDDILVLTVSSGFSGTYNSAVQA
                     AEMVDRPVHVLDSRSAEMGSGLILLEALRCIDEGGDFDEVRSVTERAIEQADVLFAVG
                     TLEYLARSGRIGRAQRLVGTALDIRPVLRVVGGEVTPFKRTRGRRRQMAAIVEQVRPA
                     VAAGRQLYFGHIAAPELVSELAETLGVEEKLVAEIGGVVGSHVGLGAYGVAHL"
     gene            8086..10200
                     /gene="recG"
                     /locus_tag="AC16CM_09900"
     CDS             8086..10200
                     /gene="recG"
                     /locus_tag="AC16CM_09900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3140184.1"
                     /translation="MPGIELPDRTTLTDLRKRPVTELTGVGPRIESALGDLGIASMAD
                     MVSHYPSRHEDLSNVKRISDLRVGERATIFGTVVSTRPVGRPVRGRAPGFSAQLYDGT
                     GYIPVTVWGRSWLLNQLHAEARVVVSGEVQSRYGIQLAAKSIEVIDDRSGEEGPHAGR
                     FVPVYPVNKNIQTRRMRTLIHRALDAAGRILDPLPADVVARHGLPELHDAIHEVHFPT
                     ERARLRAAMRRLIFHELFLIQAGLAARKARMERTETGDRHRGDGSLLNPFLKGLSFGL
                     TGAQERVVSEVLADMRKEKPMRRLLQGDVGSGKTVVAVAALLTAVEAGGQGAIMAPTE
                     VLAEQHFLSMSSALADLPVEVVLLTGSQGAAERRTALERIALGEAHVVVGTHALIQKG
                     VDFQNLSLVVVDEQHRFGVGQRTVFREKGTTPDTLVMTATPIPRTLSLTLYGDLEVSL
                     IDELPPGRKPVETTIIDPAERLGAYQEVRSELEDGRQAYVICPLVEESEALEDVRAAE
                     ELKGELAEDIFPERQVGLLHGRMKAQDKREVMARFRAGEIEVLVATVVVEVGVDVPNA
                     SAIVIEGAERFGLSQLHQLRGRVCRGLHPPKCFLVGAPKTDDSERRLEALAEHQDGFK
                     LSEVDLAIRGEGTLFGSRQSGMPDLKVAKLLRDMDVLVEARREAFALVAADPTLKRPD
                     HRPLRREIRELLGADVEWLFKE"
     gene            10306..11352
                     /gene="coaD"
                     /locus_tag="AC16CM_09905"
     CDS             10306..11352
                     /gene="coaD"
                     /locus_tag="AC16CM_09905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF015559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3140185.1"
                     /translation="MRIISGSARGTRLATVPRGVRPTADRVRESLFNALGQYFDGGSV
                     LDLYAGTGALGIEALSRGFDRAVFVERDRKAREVILDNLRRTRLPDGAEVIGGGVDGV
                     LVDLERRGEVFDLIFMDPPYRIAAEVGGNLHRLSSLLAPGGRVVIESGEAPVEVVGGR
                     KGVTRRYGGTFITILERSELTMIVAVCPGSFDPVTTGHVDVIRRACKIFDHVVVAVGA
                     NRRKQARLDAVQRARLIEQATADVDNVSVKVMDGLLVEFAREQGAGAIVKGLRAVSDF
                     ESEFEQAQLNRTLFPELETVFIMASAEHSFLSSSAVREIASLGGDVRGLVPDVILETV
                     TEIYSGRDDKIMGS"
     gene            11374..11829
                     /locus_tag="AC16CM_09910"
     CDS             11374..11829
                     /locus_tag="AC16CM_09910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR3140186.1"
                     /translation="MDVLVLIDRLEELVEDARSFPGFGNTAMIDRDAAFDIIDQMRQT
                     IPEELKQARWIVKERQAMLDEARSESDRIIRSAQDEAEKITSEEEVLKQAEKRSAEIM
                     EDARRREREIRLGAEDYADEVLANLEDNLGKLLEAVQRGRTKLQGVQEQ"
     gene            11850..12308
                     /locus_tag="AC16CM_09915"
     CDS             11850..12308
                     /locus_tag="AC16CM_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3140187.1"
                     /translation="MKRLIPLRRPGAEVGDRHEEHAEFSVEPFDLYGRHHEVTKAEAA
                     VAVTRLSEGLHLDVLVTANVLTTCDRTLEPTELELEFGDSELLSGGDDTELSVEDWKL
                     DLARYTERALPTEVPMQVFAPGSEPVEAEDTGDEVDPRWRGLDDLNVSGF"
BASE COUNT         2306 a         3681 c         4305 g         2084 t
ORIGIN      
        1 gggagcagat acggtcgcct gtggtggtga tggcgccgga tctccagagc gcacgcatcc
       61 ccgcgtactc tgatgaggca aacgtggtga gtcgtagggg cagcctggtg ttgcgcgtgc
      121 ttcccgagct ggaggagagg gttcccgggg agatcgaggt cccgcaagga tcgctcgacg
      181 tcaagagctt tttctacaat tccacgctgg agaaacggat ggagatcctg cgccgcaacg
      241 aggttgacta tgtgatgatc ccgaacgact ctccgctggt ggaaacgctc gagcgtcttc
      301 ccgggatgag ccgcgtagat acgccgagcg agaggtacgt tctactcgcc gtggaccgca
      361 gcaagctacc gggttgaccg gatgcagccg tcccgagaag aggaagtatt gttctgtcgg
      421 atagaccccc tggatccagg ttctagaaag gaacgctctt gacgtatttt ggtgagctgg
      481 gtgacagggt ggtcggtgcg ccgtcgatac tggcggccga cttcgcgcat ctggccgagg
      541 aggtccggcg ggcgcagagg ggcggtgccg acctggtcca cttcgatgtg atggacaacc
      601 attacgttcc caacctgacg gtcggtccca ttgtcgcggc ctcgctcgcg gcggcaatag
      661 acctccccat cgacgcccac ctgcagatgc gagacccgga caacctgatc ccctctttca
      721 tagatgccgg cgtcgcgagc atccaggtcc agcccgaggc cgtaacccac ctgcaccgta
      781 cgctcgagct gatccggtcg ggaggctgcg aggcgggcct ggccctgaac ccgacgaccc
      841 cgccagagct gatcgagtgg gccctaccgg ccgtggacta cataaacgtg atgagcgtca
      901 accccggctt cggaggacag gacttcatcc ccgagatggt cgagaaggta aggcgcgtca
      961 aggagatgac ccgcgtcccc ctactggtag acggcggcat caccgaagag acggcccccc
     1021 taatggtcga agccggggcc caggtcctga tcgccggctc cgccgtcttc aacggagacc
     1081 cggccacagc gatgcgcaag atcatcgaag ccggcaggtc gggacgctcc ggctaacgcc
     1141 taagctccta agcacgctac gtccttctgg ctacgcttgt cagaacacta cggccctacg
     1201 ggccttcgtt tgtcagcacg ctcggcttcg cctcgcttgt cggcgcgctt cgcccggctc
     1261 tgcctggctc gctttcggcc aattccgctg cggcctgcac cctgccgctc taaagattcg
     1321 ctgcttggaa gaggtgggtt gtcgaagcta ccgacgcgaa tcgagacgac tagccgaaag
     1381 cgaggcgaag ccgaagcgtt gctgacaagc gagggcgcta gcccgagtgt gctgacgagc
     1441 ggaggcgtta gccggagcgt gcggaagcgc gaagcgcttc cttgttgaag acttgctggt
     1501 gtgatagcgt ctccatgcta actttcgggg gcgggtggaa ttcccgcacc ggcggtgagc
     1561 ccgggcggtt tgcagaatcg ttcggggagc ccgcgaacct ctacggaggt tgagccggtg
     1621 aaggtccatt ctggaccgta gtccggcgcc gacggtcaga gtccggatgg gagaaaggaa
     1681 aacgtgctgc gggattcgat gatgggtctt gcccgctgcc tcgccgagcg cgggcgctat
     1741 acggttgcgc caaaccccct ggtgggtgcc gtgatcgccc tggatggcaa ggtcgtgggg
     1801 gagggttggc atcgcaggac cggtgaaccc catgccgagg tagcggccct tgcggaggcc
     1861 ggggtgtcgg cgcacggggc caccatgtac gtaacgctgg aaccatgcaa tcatttcggc
     1921 aggacgccgc cctgtgtgga gagcatcctt cgggctggca tctcgcgtgt cgtggtcgga
     1981 catcttgacc ccaaccccag gatgcggggc cgcagtgtcg cgttgttgcg tgaggccggc
     2041 gtcgaggtcg aggtgctgga tgatctcgcc ttcgaccgcc agaacgaaca gtttttccac
     2101 cacatgagca cgggccgtcc tttcgtccac ctcaagctcg ccgccaccct ggacggcagg
     2161 atcgccgccg ccggagggga cagcaagtgg gtgacgggtg agttggccag gcaacgggcc
     2221 cacgagctca gggccgaggc cggggcggtg ctggtcggcg ccaacacggc acgtacagac
     2281 gacccgatgc tgacggcccg tgaccttcca gcgccgccac ccagcatcac gcgcgtggtc
     2341 cttgacccca gcctcactat tagcccggag agcaggctcg cgaacacggc ggacgtggct
     2401 cccgtggtgg tcttcgctgc ggagacggcg ctcgacggtc ttcagaagcg attggaggac
     2461 cgcggcgtcg aggtggttgc cgcgccggac gatgagggga acctggacct gggccgcgtg
     2521 ctcgagaatc tcggagggcg ggggatcaaa ggtctcctgg tcgagggtgg cggggagacg
     2581 gcgacgcgat tcgtgaggaa agggctcgcg gacaagatga ccctgttcta cgcacccagg
     2641 ctcctcggct cagagggcgt gcccatgatc ggggctctcc gtgccacgaa ggttgccgag
     2701 tcgctccggt tttcggtgtc tgatgtagag aagatcgggg acgatgtggc cgtaacgctg
     2761 tatccggacc tcgccggaga ggaggaccgt gttcaccggg cttgttgagg aagttggtgt
     2821 catctcgtcg ttagaggagg gcgagatgct gcgcgtggtc atctctgccg accgggttac
     2881 gggggatacg cgggctggtg atagcatctc ggtgaacggg gcatgcctga ccgttggcga
     2941 gtccgatgga gagatgctcg cgttcttcgc catgcctgag accctgaggc gcaccgcgct
     3001 ccgcactcta gtcgtaggga gccccgtcaa cctcgagcgg gccatggccg ccggcagcag
     3061 gttcgggggg cacatcgtcc agggtcacgt ggacggtgtg ggagaggtgc tcggcgtgcg
     3121 gcccgagggt gaggcggaga tctgggagtt cggggcaccg gattcggtgc tcaggtactg
     3181 tctcgagaag gggagcatct gtgttgacgg aatcagcctg accatagtct cggtcgggaa
     3241 agattcgttc accgtttcga tcctgcccca gacccgggcc aacacgaacc tgggagctct
     3301 gggtcagggt agcagggtaa acctggaggc cgacgtagta ggcaagtacg tagagcggct
     3361 gttggagccg cggatgacag agtcgttttc gagaggagcg tagaatatgc cgttcagtcc
     3421 gatagaggag atcctggaag acatcaagca gggcaagatg gttatcgtct gcgatgacga
     3481 ggaccgggag aacgagggcg atctgaccat ggccgccgag ctggtcacgg ccaacgatat
     3541 taacttcatg gccgcccacg gccggggtct gatctgcctt ccgatggccg gcgagatcgt
     3601 ggaccgtctc gatatccccg agatggttac tcacaatgcc tcgcgcatgg gaaccgcgtt
     3661 cactgcgtcg atagaggcca gagagggcat tacgacaggc atctccgcag ccgaccgggc
     3721 gcatacctgc cgcgtcgccg tggacgacgc cacagggcca gaggaccttg cgatgccggg
     3781 ccacgttttc ccgctcaggg ccaagcccgg cggcgtgttg cagcgggcgg ggcagacgga
     3841 ggcggccgtc gacctggcca ggcttgccgg catgactccg gccggcgtca tctgcgagat
     3901 catgaaagag gacggcacga tggccagggt tcccgatctg gagaagttct cggccgagca
     3961 tgatgtcaag ctcgtaaccg tggcccagat catcgagtac cgccacgcct acgagacgca
     4021 ggtcagctgc gccgtcgaga cgaagctgcc cacgccgttc ggcgagttcc gccttcgcgc
     4081 ctacgagaac gagatcgatg agctcaccca cgtcgccctg gtgatgggcg agcctgaggg
     4141 caaggacgat gtcctggtca gggttcactc ggcatgtctc acgggcgacg ccctgcactc
     4201 cctccgatgc gactgcggcg agcaactcga gtcagccatg aggatcatcg ccgaggaggg
     4261 cgagggcgtg atcgcctaca tgcagcagga gggccgtggt atagggctac tcaacaagat
     4321 gaaggcctac cacctgcagg acgagggcat ggacaccgtt gaggccaacc agaagcttgg
     4381 cctggccccc gacttgagag actatggcgt aggtgcccag atactgaagg atctcgacct
     4441 gcggcgcatt cgcctcctga ccaacaacct gaccaaggtc gtcggcctca ggggcttcgg
     4501 gctggagata accgatcgcg tcccactcga gatcgagccc aacggctaca acgaacgcta
     4561 cctgaagacc aagcgcgaga agctgaacca cgttttcgag aagttctagg gtgcaaagtc
     4621 ggttcacgaa ccgcccaggc aaatacaggg gcggtttgtg aaccgccaga ggcaggaggc
     4681 ccgcgtggag ggagtcaatt acatcgaagg ccatctcacc gccgggggga aatccttcgg
     4741 catcgtggcc tcgcgcttca acgacttcat cgtgaaggcg ctattggatg gcgcgcttga
     4801 tgcgatcaga cgccacggcg gcgacaccgg ggctatcgat gtagcctggg tacctggctc
     4861 ctacgagata cccatcgccg ccagggagat ggccctatcg ggccgctacg acgccgttat
     4921 ctgtcttggt gccgtgatcc ggggtgccac ggcccacttc gactacgtgg ccggcggagc
     4981 cgccaacggc atcgcctcta tagcgctcga gacgggaacg cccataatct tcggcgtaat
     5041 caccaccgaa acgatagaac aggccataga acgcgccggc accaaagcgg gcaacaaagg
     5101 ctccgaagct gccgtctccg ccatagagat ggccaacctg atgccgcagg tgcgcaaagg
     5161 tccttgacct ttgcgcgcac gctccggcta gcgcctccgc ctgttcagca cggcacttcg
     5221 cgcctcgtca gcacgccggc tccgcttcgc tctgctgaca agctgccgca ggcagcgtgc
     5281 ggcgagcgtt aagcgctcgc cagcgccttc tgtaccttga aggccttcag gcaggaggtg
     5341 cagatccatt cgcggcgggt ggatgggccc tcctggatgc ggactttctg caggttcgga
     5401 tttctgcggc ggcgcgtcgc cctcagggag tggctgcgtg cgttgccgga gtcgggcctc
     5461 ttcccgcagg agtaacagac tttagccacg atacttctcc tgtgctagga taccggtcga
     5521 atttggggac tccggaaaac gcgatacgca agccgcctcc ggacgtcggg cgaagattgt
     5581 accagattgc cgacgttagc ctagagcgga gtatgagccg cccgcgggcc ggtgtgtcgg
     5641 gagcctgatg agaggagcga tggaaggtct tttgcgatgt cggtagaggt gtccctgctg
     5701 gtcgccgcgg cccacgcggc cgtcaaggca caggtggcca ggatcaacga cctgaacgtc
     5761 tacccggtcc ctgacggcga tactggtacc aacatgctgc tgacgttgga atcggcgctc
     5821 gaggagactc ggggtgaggc ttacgccagt tcggaagagg ccagcaaagc cgtggcccgg
     5881 gccgccctga tgggggcgag gggcaactct ggcgtgatcc tctcccagat gatacggggc
     5941 gccgcagaag ccctgacgga ccgctcctcc cttgatgcgg agaccttcgc agccgggctg
     6001 gagggggcgc gtgggcgggc ctactcctcc gtacgggaac cagtcgaagg cacgatgctg
     6061 accgtaataa aggacgccgc cgtggcggcg cgagaggtga tcgacggtgg agaggacgac
     6121 ctgcccacca cggtcgaggc agcgcgaagg gcagcccacg cttcggtgcg tcgaaccccc
     6181 gagttgctgc cggtgctgcg cgaggccggg gtggtcgacg cgggtgggtt gggcgtggcc
     6241 gtggtacttg acggtgttta cgcttgcgtc acgggtgagg agatagaggt tcccgaggaa
     6301 gtggacgaaa acgatacccc ggacctcgat gcgatccacg ctcaggagga ggcgtggggg
     6361 tattgcacgg agtttctggt cgacggcttc agcggcgatg ccgaggagtt cggcgagtac
     6421 atctatgcct caggtaggag cgtcttcgtg gtagccgacg acgacgtggt gaaggttcat
     6481 ctgcacaccc aggacccggg agaagcactt acctacgccg gtcgtttcgg acgcctcgcc
     6541 ggcgtcaagg tggacgacat ggaggcccag gtccgctctc gtgaaaagga aatccctcga
     6601 agaccccccg ccaacctcgg ggtggtcgcg gccagccgtg gagagggtaa tcgaaggctc
     6661 ttcgagcaga tgggggctgt ggtgatcgag gggggacagg gcgagaaccc gagcgccgca
     6721 gacctcgcca gggccgtcga ggagacggga gcgccggtgg tcgtcttgct gccgaacaac
     6781 aagaacatcg tgccgaccgc cgagcaggta ggcgagctgg tcggggttgg gacgcatgtc
     6841 gtgccgacca cgagcatcgc agccggcctg gccgttatgg tcgggtacga tgccgagggc
     6901 gagccggatg gcgtggttga ggagatgctg gagatctaca gctcgctccg cgctgcggag
     6961 gtcacgatct ccgtgcgaga cgcccgcatc ggagatcgcg aggtcccgga gggagcgttc
     7021 atagggttcc tggccggcga gctcatagcc gtggagaaga ccctggcggg ggccgtaatc
     7081 gcgctggctg acaagatcgt cggcgagggc gccgacttcc tcacgctcct gaaaggtgag
     7141 gatctctccg aggatgacct cgaggccatg ctgcaggcga tccacgagct ggatgaagac
     7201 ctcgaggtag aggtcaggga cggtggacag ccgctgtatc cggttcagat ggtggcggaa
     7261 tgacaacggc ggtcgtaacg gattccacca cgagcctggc cccgggtgtt ctggagcgtc
     7321 ccgacgtaag gatggttccg cttaccttca actttggctc ggacgagacc tatagggaca
     7381 aattggacat gtccaacgcg gagttctacg agaggctgcg cagctctgac gagttcccga
     7441 ccacggccca gccttcgccc ggagccttcg tgcagaccta cgaggcgctc tccgactacg
     7501 atgacatcct ggtcctgacg gtctcgtccg ggttcagcgg cacgtataac tcggccgtgc
     7561 aggcggctga gatggtcgac cggccggttc atgtactcga ctcgaggagc gccgagatgg
     7621 ggtctggttt gatcctcctt gaggcactcc gttgcatcga cgaagggggg gacttcgacg
     7681 aggtcaggag cgtcacggag agggcgatcg agcaggctga cgtcctcttc gcagtcggca
     7741 cgctggagta cctggccagg agcgggcgaa tcgggagggc gcagcgcctg gtcgggacgg
     7801 cgctcgacat caggcccgtc ctccgggtcg tcggcggaga ggtgacgccg ttcaagcgaa
     7861 cccgcgggcg cagacggcag atggccgcca tagtggagca ggtcaggccg gctgttgcgg
     7921 ccggacgaca gctctacttc gggcacatcg ccgcgccgga actcgtctca gagctagccg
     7981 agaccctcgg tgtggaggag aagctcgtcg ccgagatcgg tggtgtcgtc gggtctcacg
     8041 tcggcctggg agcctatggc gtggcccacc tctagcaccg gcgacgtgcc ggggatagag
     8101 ctccccgacc gtacgaccct tacggacctg cgcaagcggc cggttaccga gctcaccggt
     8161 gtcggacctc gcatagaatc cgcgctcggc gacctcggca tagcctcaat ggccgacatg
     8221 gtctcgcact acccatccag gcacgaagac ctctccaacg tgaagcggat ctccgacctg
     8281 cgcgtgggag agcgggccac catcttcgga acggtagtct ccacgcgtcc cgtgggcagg
     8341 ccggtcaggg gacgggcccc ggggttctcg gcccagctct acgacggcac cggatacatt
     8401 cccgtgacag tctggggaag gagctggctg ttgaaccagc tgcacgccga ggctcgggtg
     8461 gtggtctcgg gcgaggtcca gagccgatac ggtatccagc tggccgccaa gagcatcgag
     8521 gtgatagacg acaggtccgg tgaggaggga ccgcacgccg ggcggttcgt tcccgtctac
     8581 ccggtcaaca agaacattca gacccgcagg atgaggaccc tgatccaccg cgccctcgac
     8641 gcggctggcc gcatcctcga cccgctgccg gccgatgtgg tggcccgtca tggcctgccc
     8701 gaactgcacg acgcaataca cgaggtccac ttcccgacgg agcgggcgcg cctgcgggcg
     8761 gccatgcgca ggctcatctt ccacgagctc ttcctgatac aggctggcct cgcggcccga
     8821 aaggcgcgaa tggagaggac ggagacgggc gacagacacc gcggcgacgg cagcctgctc
     8881 aacccgttcc tgaaggggct gtcttttggc ctgacaggcg cccaggagcg ggtcgtgtcc
     8941 gaagtcctgg ccgacatgcg caaggagaag ccgatgcgac gcctgctcca gggcgacgtg
     9001 ggcagcggca agaccgtggt cgccgttgcg gctctcctca cggccgtgga ggcgggcggg
     9061 cagggagcca tcatggctcc gaccgaggtg ctggcagagc agcacttcct gtccatgtcc
     9121 tcagccctcg ccgatctgcc ggtcgaggta gtcttgctga caggctccca gggggcagcg
     9181 gagcggcgga cagcgctgga gcggatcgcc ctgggcgagg cgcacgtggt cgtcggcaca
     9241 cacgccctaa tacagaaggg cgtggacttc cagaaccttt cgctcgtggt ggttgacgag
     9301 cagcaccggt tcggggtcgg ccagcgtacg gtcttccggg agaagggaac gacgccggac
     9361 acgctggtca tgaccgcgac tcccataccg agaacgctct ccctgacgct ctacggagac
     9421 ctggaggtct ccctgataga cgagcttccg ccgggaagaa agccggtcga gacgaccatc
     9481 atagacccag ccgagcgcct gggagcctac caggaggtca ggagcgagct ggaagatggc
     9541 cggcaagcct atgtgatctg ccccctggtc gaggagtccg aggcgctgga ggacgtaagg
     9601 gccgccgaag agttgaaggg cgagcttgcc gaggacatct tccccgagag gcaggtgggt
     9661 cttctgcacg gcaggatgaa ggcgcaggac aagcgcgagg tcatggcgcg gtttcgggcc
     9721 ggggagatcg aggtgctggt ggcgaccgtc gtggtcgagg tcggcgtgga cgtgccaaac
     9781 gctagcgcga tcgtgatcga gggcgccgag cgattcggcc tctcccagct ccaccagctg
     9841 aggggccggg tctgtagggg gctgcatccc ccaaaatgct tcctcgttgg tgccccgaag
     9901 accgacgact ccgagcggcg cctcgaagcc ctcgccgagc accaggacgg gttcaagctc
     9961 tcggaggtgg acctggccat ccgcggcgag ggaacgctgt tcggtagccg ccagagcggc
    10021 atgccagact tgaaggtggc caagctgctg cgcgacatgg acgttctggt cgaagcgaga
    10081 cgtgaggctt tcgccctcgt ggccgcggac ccaaccctca agcgcccgga ccatcggccc
    10141 ctcagacgag agatccgcga gctcctcggc gccgacgtgg agtggctttt caaggaatga
    10201 cggtaggcca gccttgaatt gtcagccgct cgaactcatc tcaacccatc gtagggttgg
    10261 gtttcgaact cggccctgcg gcctattctc tgaagctggg cgagcgtgag gatcatctcc
    10321 ggatcagcgc gtggcacccg cctggctacg gttccgaggg gagtgcggcc aaccgcggac
    10381 cgcgtgcggg agagcctctt caacgcgctc ggccagtact tcgatggtgg gagcgtcctc
    10441 gacctctacg cggggaccgg tgcgctcggc atagaggccc ttagccgcgg gtttgacagg
    10501 gcggttttcg tcgagcggga caggaaagct cgcgaggtga tcctcgataa tttgcggcga
    10561 acccggctcc cggacggggc cgaggtgata ggcggcgggg tcgacggcgt gctcgtagac
    10621 ctcgagcggc ggggggaagt cttcgatttg atcttcatgg acccaccata tagaatcgcg
    10681 gcggaggtcg ggggcaactt gcaccggcta agctcgctgc tcgcgcccgg aggaagggtc
    10741 gtgatcgaga gcggtgaggc tccggttgag gttgtgggag gcaggaaagg ggtaacgcgc
    10801 cgctatggcg gcacgttcat aaccatactt gagcggtccg agctgacaat gatcgtggct
    10861 gtgtgtcccg gaagcttcga ccccgttaca acgggccacg tggatgtgat ccggcgggct
    10921 tgcaagatct tcgatcacgt ggtggtggct gttggcgcca accgccgcaa gcaggccagg
    10981 ctcgacgccg tacagcgggc gcgattgata gaacaggcca ccgctgacgt ggacaacgtc
    11041 tccgtcaagg tcatggacgg cctgctggtg gagtttgcca gggagcaggg cgcgggagcc
    11101 atcgtcaagg ggctcagggc ggtctctgac ttcgagtcgg agttcgagca ggcgcagctg
    11161 aacagaaccc tgttcccgga gctcgagacg gttttcataa tggcttctgc cgagcacagt
    11221 ttcctgtcct cgagcgcggt cagggagata gcctcgcttg ggggggacgt gcgcgggctc
    11281 gttccggacg tcatactgga gaccgtgacg gagatatact ccggcaggga cgataagata
    11341 atgggatcgt aggcgaagga tagcgagtag aagatggacg tactggttct aatagacagg
    11401 ttggaagaac tcgtcgagga cgcgcgcagc ttccccggtt tcggcaacac cgccatgatc
    11461 gacagggacg ccgccttcga catcatagac cagatgcgcc agaccatacc cgaggagttg
    11521 aagcaggcgc gttggatcgt caaggagcgg caggcgatgc ttgacgaggc gcgcagcgaa
    11581 agcgaccgaa tcattcgcag tgcccaggac gaggccgaga agatcacctc cgaggaggag
    11641 gtcctcaagc aggccgagaa gcggagcgcg gagatcatgg aggacgcccg ccgccgcgag
    11701 cgcgagatac ggctcggcgc cgaggactat gccgacgagg tgctcgccaa cctggaagac
    11761 aacctgggca agctgctcga ggccgtgcag cgcggccgca ccaagctgca gggcgtccag
    11821 gagcaataac tcgcggtaga ctagccacca tgaagcgttt gatccctctc aggcgacccg
    11881 gtgcggaggt cggagacagg cacgaggagc atgccgagtt ctccgttgaa cctttcgacc
    11941 tgtatggccg ccaccacgag gttacgaagg ccgaggccgc cgtggccgtc acgcgcctct
    12001 ccgagggcct gcacctcgat gtgctcgtca cggccaacgt gctgaccacc tgcgaccgga
    12061 cgctcgaacc aaccgagctc gaactggagt ttggtgattc ggagctgtta tcggggggag
    12121 acgataccga gctctccgta gaggattgga agctcgacct ggcgcgctac accgagcgcg
    12181 cacttcccac agaggttccg atgcaggtgt tcgcgccagg ctccgaaccc gtggaggccg
    12241 aggacaccgg cgacgaggtg gacccgcgtt ggcggggcct cgacgacctg aacgtctcgg
    12301 ggttctagac cgtaagcaag tagcaaccag gaggtaatct gatggccgtt ccaaagaaga
    12361 agacgtcgcg ggctcg
//
LOCUS       DBNGVD010000368         8277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4641481, whole genome shotgun sequence.
ACCESSION   DBNGVD010000368 DBNGVD010000000
VERSION     DBNGVD010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4641481"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="AC16CM_09920"
     CDS             <1..373
                     /locus_tag="AC16CM_09920"
                     /inference="COORDINATES: protein motif:HMM:NF036579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3140188.1"
                     /translation="LGSVGEWLYRYVAGIDLGSPGYGHIVIRPHPGGDLTFARGEHDS
                     VRGRISSSWRIENEQVVLEVLVPPNTTATVHIPSTEGVSEGGRPVEEADGVEVLRAGG
                     GETVVTVGSGRYEFSGGLVRG"
     gene            370..1863
                     /locus_tag="AC16CM_09925"
     CDS             370..1863
                     /locus_tag="AC16CM_09925"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR3140189.1"
                     /translation="MSAAYLAVDLGAESGRVVLGRFDGERMSVEEVHRFPNTPVGLPD
                     GLHWDVLKIYSEIKEGLAKGLHGGEIEGIGVDSWGVDFGLLDRDGALVSNPHHYRDAR
                     TEGMIEEAFGLMPKEDLYSLTGIQFIPLNTLYQLLAMRGSPLLEAADSLLLIPDLMNY
                     WLTGEKACEYTNATTTQLLDLEKGDWSRDLLERMGLPTSMLTPIIQPATELGSPLPGV
                     AEEIGGGPPVFSVASHDTASAVVAVPAEGQDFAYISSGTWSLLGVELSSPVVTREALK
                     ANFTNEGGFGGRTRLLKNLAGLWLLQECRRQWSREGHEYSYEELANLAADAPSGPLVD
                     PDHPAFLPPGDMATRIRSFCEETGQEVPEEPAAVARCVFESLALKYRHAIDEAEKLGG
                     RAIGKVNVVGGGSRNELLCQLTADATRRPVLAGPAEATALGNLMVQAYARGRLSSLEE
                     IRESVRRSVEVCEYEPQGVEGDWQESYEKLGEVVRAAPRLDLEGESA"
     gene            1860..3410
                     /locus_tag="AC16CM_09930"
     CDS             1860..3410
                     /locus_tag="AC16CM_09930"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3140190.1"
                     /translation="MREPEGTRAAAAEETPVFRLDGVTKRFGGVVAVEDVAFELRRGE
                     VHALVGENGAGKSTLMKIVDGLYGPDEGTLEVGGEAASFSSPRDAEASGIAMIPQELD
                     LFPELSVAENLFVGRRRPRTGWGGLDWETMRGEARKRLEALGVGLDVTSSVKGLSVAE
                     QQIVAIARALVGEARAVIMDEPTSSLTDREVRQLFSIIEDLAAEGVGVVYISHRMDEI
                     FEISDRITVLRDGHHIETAPASELDAEELVRLMVGRPLHELFTRSESSPGETALEVRG
                     LSRDGEFEGVDLTLRRGEIVGLAGLVGAGRTELAQSIFGIRHPDAGEISVEGEEVRIH
                     SPQAAMEKGIFYVPEERRSQGLILPFSIKNNITLSILDRISRLGFVSRAERDIADKFM
                     GELSIRGADVSAPVSRLSGGNQQKVVVAKSLAREPNVLLLDEPTRGVDVGAKSEIYRL
                     MDDLAREGKAILLISSELEEVLSMADRILVMREGRISGEFTRGEATQEGVMTAATGGP
                     EREVYEDE"
     gene            3403..4389
                     /locus_tag="AC16CM_09935"
     CDS             3403..4389
                     /locus_tag="AC16CM_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140191.1"
                     /translation="MSEARTVNAGGGEGFRRVLGRPEAPALAFLILLVVAFSVLSGEF
                     LSVSNLESILTSVAVLGVIALAVNQVVLAGEIDISTGSMLGLCAVAGGAAAQTTGGFL
                     VPLVAAVAVGALCGAINGTLVTLGRIPSIIVTLGMLYALRGVILLVTGGTWITGIPND
                     SRFLGMGSVLGLSVPVLVLFGLFVVMEVVGRHTTWGRDVYAVGGNRRAARFAGLPTDR
                     VRFSTFVLVGVFVGIASMIYMGRAGSVQTNTGTGLELQVIAAVVIGGTSISGGRGSAL
                     AALTGAVLIGVILNGLVLIGVPGIWQQAVLGALILLAITTDVVRRRLVGDAA"
     gene            4386..5345
                     /locus_tag="AC16CM_09940"
     CDS             4386..5345
                     /locus_tag="AC16CM_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140192.1"
                     /translation="MSAATAALRRLRFSRELVLLGVLIVLVIVMSLLSPLFFTLGNLL
                     NTSRYFVEVGLMALGMTLIIITGGIDLSVGSNLALVSVAVGFTFAAGLPLPLAILFGL
                     VVGMAAGFFNSVFITFLNLHPLVVTLGTFALFQGLAYGLTGARAVSDFPAWFAYFGQA
                     YLLGAVPGQLVIFIVAVVVVWVILSRTSFGRYVYAIGNNEEAARFSGVPIRRVKVAIY
                     TGIGLLVAVAAVIYTSRVYTARGDVGLGLELDVISAVVLGGASIYGGSGTIAGTVLGV
                     VIIAILRNGLLLAGVPSTWQLFILGVLLLVAVFLNEFFRKGEE"
     gene            5459..6490
                     /locus_tag="AC16CM_09945"
     CDS             5459..6490
                     /locus_tag="AC16CM_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3140193.1"
                     /translation="MSGRTRKQRAFSVILLAVLVAAAASCGVGQQPSQGNGGGGGGPS
                     KICMMPKLVGIPYFNASEKGAKEAAKELDVELVYDGPTEAKAALQSQMIEQFIQQQCG
                     AITVAANDPNALAPAMKKAGDAGIATGAWDADVAPDARDVFVNQATFKAVGYDMVDVM
                     AKQTDGKGEFLVVTGSLTAPNQVAWLKEMRARMKEKYPGMSIAAVEPGEEDLQKGIDL
                     TKNYLRANPDTAGVFGITSVALPGAAEAVEQLGREGEVPVTGLSTPNEMKPYLDSGAV
                     EEFVLWNPVDLGYLAVYVADAQINGDLPKSGTFEAGRLGKVEMISSDEVLLGPPLIFS
                     KENIDDYDF"
     gene            6610..7821
                     /gene="rhaI"
                     /locus_tag="AC16CM_09950"
     CDS             6610..7821
                     /gene="rhaI"
                     /locus_tag="AC16CM_09950"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR3140194.1"
                     /translation="MTIRQDYEDLTRRLEERGVDLASVEDRLRAQRLETPSWGYGDTG
                     TRFAVFPQRGVPRDPFEKMADAAQVHGLTGVCPSVAIHIPWDRVDDYGKLKEHAESLG
                     LRLGAVNPNVFQDPEYKLGSVCNPDPAVRRKATDQLLECVAVAGETGSDVLSLWFADG
                     TNYAGQDSFTERRHRMVGCLEEVYEAMPGDMRMLLEYKPFEPAFYHTDLADWGAALTV
                     SQRLGERAEVLVDLGHHAKEVNIEYVVASLLDEGRLGGFHFNNRKYADDDLIVGSVNP
                     FELFLIYVELVAAEERSQTRIDYMIDQTHNIEPKIEAMLLSVMNLQEAYAKALLVDRP
                     ALTEARHSGNVLGAHRILLDAYATDVRPLCATVRESLGGAADPISAFKASGYAERMAE
                     ERGEGAGAGWN"
     gene            8018..>8277
                     /locus_tag="AC16CM_09955"
     CDS             8018..>8277
                     /locus_tag="AC16CM_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3140195.1"
                     /translation="MDTTTPGTTDNLWNAREAEGLSDLEALAYRSNLLGRDRAVANYG
                     GGNTSTKSRAKDHAGREVDVLWVKGSGSDLATIEADQFTGLKL"
BASE COUNT         1435 a         2578 c         2878 g         1386 t
ORIGIN      
        1 tctcggctcg gtcggcgagt ggttgtaccg ctacgtggcc gggatagacc tcgggtcgcc
       61 aggctacggc catatcgtta taaggccgca tccgggcgga gacctcacgt tcgccagggg
      121 cgagcacgat tcggtacggg gcaggatctc cagctcctgg cggatagaga acgaacaggt
      181 cgtactcgaa gtgctcgtcc cccccaacac cacggccacg gtgcacatac cctccacgga
      241 aggcgtctct gaggggggca ggcccgtgga agaggccgac ggtgtggagg tgctgcgggc
      301 cggcggtggg gagaccgtcg tcaccgtcgg atccggacga tacgagttct cgggcgggtt
      361 ggtgcggggg tgagcgcggc gtacctggcg gtcgacctcg gcgccgagag cggtcgcgtc
      421 gtgctcgggc gcttcgacgg cgagaggatg tcggtggagg aggtccatcg cttcccgaac
      481 acgcccgtcg ggctgccaga cgggcttcac tgggacgtcc tgaagatcta ctccgagatc
      541 aaagaggggc ttgcgaaggg gctgcatggc ggggagatag agggtatcgg ggtcgattcc
      601 tggggcgtgg acttcgggct gctggaccgg gacggggcac tcgtctcaaa cccccaccat
      661 taccgcgacg cgcgcaccga gggcatgata gaggaggcct tcgggcttat gccgaaggag
      721 gatctctaca gcctgacggg catccagttc atcccgctca acaccctcta ccagctactc
      781 gccatgcgcg ggtcaccgct cctcgaggcc gcggactccc tgctcctgat ccccgacctg
      841 atgaactact ggctgaccgg cgagaaggcc tgcgaataca ccaacgccac gacgacccag
      901 ctcctggatc tcgagaaagg cgactggtcg cgggacctgc tcgagaggat gggcctcccc
      961 accagcatgc taacgcccat catccagccc gccaccgagc tgggatcccc gctgcccggt
     1021 gtcgccgaag agatcggcgg gggaccgccc gtgttctcgg tcgcctccca cgacacggcc
     1081 tcggctgtag tagcggtgcc ggccgagggc caggacttcg cctacatctc cagcggcacc
     1141 tggtcgctcc tgggggtaga gctctccagt ccggtggtga ccagggaagc gctgaaggcc
     1201 aacttcacca acgagggcgg gttcgggggc cggacgcgcc tcctgaagaa cctcgccggc
     1261 ctctggttgt tgcaggagtg ccgcaggcaa tggtccaggg aaggacacga atactcctat
     1321 gaggagctgg ccaacctcgc cgcggacgcg ccgtccggcc cgctcgtgga tcccgaccat
     1381 cccgctttcc tgccgccggg ggacatggcc acgcgcatca ggagcttctg tgaggagacg
     1441 gggcaggagg tgccggaaga gcctgccgcc gtggcccggt gcgtcttcga gagcctggcg
     1501 ctcaagtaca ggcacgccat agacgaggcg gagaagctgg gtggccgggc catcgggaag
     1561 gtcaacgtgg tcggtggtgg ctcccggaac gagctgttgt gccagctaac cgccgacgcc
     1621 accaggcggc cggtgctggc gggaccggcc gaagcaacgg cgctggggaa cctcatggta
     1681 caggcctacg cgcgcggccg cctctcctcg ctggaggaga taagggagtc cgtgcgccgc
     1741 tcggtggaag tgtgcgagta tgagccgcag ggtgtggaag gcgattggca ggagtcctac
     1801 gagaagctag gggaggtcgt ccgggccgcg ccgaggttgg acctggaagg agagagcgct
     1861 tgagagagcc ggaaggaacc agggccgcag ccgccgagga gaccccggtg ttcaggctcg
     1921 acggggttac caagcggttc gggggcgtcg tcgcggttga ggacgtcgcc ttcgagttgc
     1981 gtcgcgggga ggtgcacgcc ctcgtcgggg agaacggtgc tggaaagagc acgctcatga
     2041 agatcgtgga cggcctctat ggtccggacg aggggaccct ggaggttggc ggggaggccg
     2101 cctcgttctc ttcgccgcgc gacgcggagg cgtcggggat agccatgatc ccgcaggagc
     2161 tcgacctttt tccagagctt tcggtggcgg agaacctctt cgtcggccgc aggaggccgc
     2221 ggaccggctg gggcggcctc gactgggaga ccatgcgggg cgaggcccgc aagaggttgg
     2281 aggccctcgg ggtcggcctc gacgtcacgt cgtccgtcaa ggggctctcc gttgctgagc
     2341 agcagatcgt ggccatcgcg cgggcgctcg tgggcgaggc gcgggccgtg atcatggacg
     2401 agccgacctc ctccctgacc gatcgtgagg tccggcagct cttcagcatc atcgaggacc
     2461 tcgccgctga gggcgtcggg gtcgtctaca tctcccatcg gatggatgag atcttcgaga
     2521 tctccgaccg aattaccgtg ctgagggacg gccatcacat agagacagcc ccggcctccg
     2581 agctcgacgc cgaagagctc gtccggctca tggtcggcag gcccctgcac gagctcttca
     2641 cccgcagcga gagtagcccg ggcgagacgg cgctggaggt gcggggtctc tcccgcgacg
     2701 gggagttcga aggggtagac ctcacgcttc gcagggggga gatcgttggc ctcgccgggc
     2761 tcgtcggggc cggacgcacc gagctggccc agtccatctt cgggatcagg catcccgacg
     2821 ccggagagat ctccgtcgaa ggggaggagg tcaggatcca ctctccccag gccgccatgg
     2881 agaagggtat cttctacgtg ccagaggagc gccgctcgca ggggctcatc ctgccgttct
     2941 ccatcaagaa caacatcacc ctcagcatcc tggaccgcat ctcccggctc gggttcgtct
     3001 cccgcgccga acgggacatc gccgacaaat ttatggggga gctctccata cggggggccg
     3061 acgtctcggc gcccgtgagc cggctctccg gcggcaacca gcagaaggtc gtggtcgcca
     3121 agtcgctagc ccgcgagcca aacgtgctgc tgctcgacga acccacgcgg ggcgtcgacg
     3181 tcggggcgaa gtcggagatc taccgcctca tggacgacct ggccagggag ggaaaggcga
     3241 tcctgctcat ctcttccgag ctggaggagg tcctctccat ggccgaccgc attctggtga
     3301 tgcgcgaggg ccgcatctcg ggcgagttca cgcgcgggga ggcgacgcag gagggcgtta
     3361 tgaccgcggc aaccggcggc cccgagcggg aggtttacga ggatgagtga ggcaaggacc
     3421 gtgaacgctg ggggcgggga ggggttccgc agggtccttg gccgtcctga ggcaccggcg
     3481 ctcgccttcc tcatcctgct cgtggtggcc ttctcggtgt tgagcggcga gttcctaagc
     3541 gtctccaacc tcgagtccat cctgacctcc gtcgccgtgc tcggggtcat agccctcgcg
     3601 gtcaaccagg tcgtgctggc cggggagata gatatctcga cgggctcgat gcttggcctg
     3661 tgcgctgtgg ccgggggagc cgcggcgcag acgaccggcg ggtttctggt gccgctcgtg
     3721 gccgccgtgg ccgtcggggc gctatgtggt gccatcaacg gtaccctggt cacgctgggg
     3781 cgcatccctt ccattatcgt cacgctcggg atgctgtacg cgttgcgtgg tgtgatcctg
     3841 ctcgtaacgg gcgggacctg gatcaccggg atcccgaacg actcaaggtt tctgggcatg
     3901 gggagcgtgc tcggcctgag cgtgccggtt ctggtcctct tcggcctctt cgttgtgatg
     3961 gaggtcgtgg gacgccacac gacctgggga cgcgacgtct atgccgtcgg cggcaacagg
     4021 agggcggccc gtttcgcggg gctgcccacg gaccgcgtca ggttctccac cttcgtgctc
     4081 gtcggcgtct tcgtcggcat cgcgtccatg atctacatgg gacgcgccgg ctcggtccag
     4141 accaacacgg gcaccggcct cgagctccag gtcatcgccg ccgtcgttat aggtgggacg
     4201 agcatctcgg gagggcgcgg ctcggcgctc gcggcgctga ccggcgcggt cctcataggc
     4261 gtcatcctga acggcctcgt tctgatcggg gtgcccggca tctggcagca ggccgtgctc
     4321 ggggccctga tcctgcttgc cataacaaca gacgtggtgc gcaggcgcct cgtgggagac
     4381 gcggcgtgag cgcggcgacg gcggcgctca ggcggctcag gttctcccgc gagctggtcc
     4441 tgctcggcgt cctgatagtg cttgtcatcg tgatgagctt gctctccccg ctgttcttca
     4501 cgttgggcaa cctgctcaac acctcacgct acttcgtcga ggtcggcctc atggcgctcg
     4561 ggatgaccct gatcatcatc acgggcggca tagacctctc ggtaggctcg aacctggcgc
     4621 tggtctccgt ggccgtcggc ttcaccttcg cggccggcct cccgctgccg ctcgccatcc
     4681 tgttcggtct cgtggtgggg atggccgccg gcttcttcaa cagcgtcttt atcacgttcc
     4741 tgaacctgca tcccctcgtg gttaccctgg gtaccttcgc cctcttccag gggcttgcct
     4801 acggcctcac gggggcccgg gccgtctcgg actttccagc ctggttcgcg tacttcgggc
     4861 aggcgtacct gctcggcgcg gtgcccggcc agctcgtgat cttcatcgtg gccgttgtcg
     4921 tggtctgggt catcctctcg cgcacgagct tcgggcgtta cgtctacgct ataggcaaca
     4981 acgaggaggc ggcccgcttc tccggcgtgc ccatccgcag ggtcaaggtg gccatctaca
     5041 ccggcatcgg gctgctggtc gccgtcgctg cggtgatcta cacctcaagg gtgtacacgg
     5101 caaggggcga cgtcgggctg gggctggagc ttgacgtgat ctccgccgtc gtgctcggcg
     5161 gggcgagcat ctacggcggc tcggggacca tcgccgggac cgtgcttggc gtggtgatca
     5221 tcgccatact gcgcaacggc ctgctcctgg ccggggttcc tagcacctgg cagctcttca
     5281 tcctcggggt cctgctgctg gtggccgtat tcctgaacga gttcttccgc aagggcgagg
     5341 agtagagcat gccaggatag ggcggttcac ggagggagtg aaaccggggc aaggcaacag
     5401 atgattctcc tgaaacctga gacctgtagc ctgaagcctc cataggggaa ggagtagcgt
     5461 gtctggcagg acgaggaaac agagggcatt ttcggtgatc ttgctcgcgg tgctggttgc
     5521 ggcggccgct tcgtgtgggg tggggcagca accgtcacag ggcaacgggg gtggcggtgg
     5581 tgggccgtcc aagatctgca tgatgcccaa gctggttggc atcccctact tcaacgcctc
     5641 ggagaagggg gccaaagagg cggcgaaaga gctagacgtg gagctggtct acgacggccc
     5701 gacggaggcc aaggccgcgc tccagtcgca gatgatagag cagttcatcc agcagcagtg
     5761 cggcgccatc acggtcgcgg cgaacgatcc gaacgcgctg gcaccagcca tgaagaaggc
     5821 cggcgacgcg ggaatcgcca cgggcgcgtg ggacgccgac gtggcccccg acgccagaga
     5881 cgttttcgtc aaccaggcca cgttcaaggc cgtcggctac gacatggtcg acgtcatggc
     5941 caagcagacg gacggcaagg gagagttcct ggtcgtgacg ggctcgctga ccgccccgaa
     6001 ccaggtcgcg tggctcaaag agatgcgcgc ccgcatgaag gagaagtacc ctggcatgtc
     6061 catagccgcc gtcgagccgg gtgaggaaga cctccagaag ggtatcgacc tgaccaagaa
     6121 ctacctgagg gccaacccgg acacggcggg cgtcttcggc ataacctccg tggccctccc
     6181 cggcgccgcc gaggccgtgg agcagctggg ccgtgagggc gaggtgcccg taacgggcct
     6241 ctccaccccc aacgagatga aaccctacct cgactccggc gctgtggagg agttcgtcct
     6301 ctggaacccg gtcgacctcg gctacctggc cgtctacgtg gcggacgccc agatcaacgg
     6361 tgacctcccc aaatcaggca ccttcgaggc gggacgcctc gggaaggtag agatgatctc
     6421 ctccgacgaa gtcctgctgg gaccgccact gatcttctcc aaggagaaca tagacgacta
     6481 cgacttctag cgtggcctag ggaaaaggcg aaacgacggg agaggaaggc aacaggcatc
     6541 agccgacagg caacgggtaa tcttcctgaa gcctgatgcc ttcttctcag gaggagagcg
     6601 atattgagca tgacgatccg gcaggactat gaggacctca cgcggaggct tgaggagagg
     6661 ggtgttgacc tcgcctccgt cgaggaccgg ctgcgggccc agaggctgga gacgccttcg
     6721 tggggctacg gcgacacggg cacgcgtttc gctgtctttc cgcagagggg tgtgccccgc
     6781 gacccgttcg agaagatggc cgacgcggcc caggtgcacg gcctcaccgg cgtgtgccca
     6841 tcggttgcga tccacatccc gtgggacagg gtcgatgact acggcaagct gaaagagcac
     6901 gcagaatccc tcggcctgcg cctcggggcg gtcaacccca acgtgttcca ggatcccgag
     6961 tacaagctcg gcagcgtctg caacccggac ccggccgtgc gcaggaaagc cacggaccag
     7021 ctgctcgagt gcgttgctgt cgcgggcgag acgggctcag acgtgctctc cctatggttc
     7081 gccgacggca cgaactacgc gggccaggac tccttcacag agcgtcgcca caggatggtg
     7141 ggatgcctgg aggaggtcta cgaggccatg cccggggaca tgcggatgct cctcgagtac
     7201 aagcctttcg agccggcctt ctaccacacc gatctcgccg actggggcgc ggccctgacc
     7261 gtcagccaga ggctgggcga gagggcggag gttctcgtgg acctcgggca tcacgccaag
     7321 gaggtgaaca tcgagtacgt ggtggcgtcg ttgctcgacg agggacgcct tggcgggttc
     7381 cacttcaaca acaggaagta cgccgacgac gatctcatcg tgggaagcgt caacccgttc
     7441 gagttgttcc tgatctacgt cgagctggtc gcggccgaag agcgctcgca gacccgcata
     7501 gactacatga tcgaccagac ccacaacatc gagcccaaga tagaggccat gctcctctcg
     7561 gtcatgaacc tgcaggaggc ctacgcgaag gcgctgctgg tagaccgccc ggcgctcacc
     7621 gaggcgcgcc actccggcaa cgtgcttggc gcccaccgca tcctcctcga cgcctacgcc
     7681 accgacgtac gtcccctgtg cgccaccgtc cgagagtccc tcggcggcgc ggcagacccg
     7741 atctccgcct tcaaggccag cggctacgcc gaaaggatgg ccgaggaacg aggcgaaggc
     7801 gcgggagcag gatggaacta aagctgtcag ctcttagagc tatcagctat tagctgtcag
     7861 ccatcagcca cgccgtggat ggaggaagcg tgttgagctt ctggaagtca cgatggaaac
     7921 caagcacctc gggagcgttt gggcatgaag atcgaagaac aaaaagctga tagctgaagg
     7981 ctgacagctg acagcgccga ccaaagggag gccaaaaatg gacacgacta ctccgggaac
     8041 gacagacaac ctgtggaacg cgcgagaggc agagggcctc tccgacctcg aggcgcttgc
     8101 ctaccggtcc aatttgctgg ggagggacag ggccgtcgcc aactacggtg gaggtaatac
     8161 ctcgaccaaa tccagggcga aggaccacgc ggggagggag gtggacgtcc tgtgggtcaa
     8221 ggggtcggga tcggacctcg cgaccataga ggccgaccag tttacggggc tcaagct
//
LOCUS       DBNGVD010000369         5443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1908239, whole genome shotgun sequence.
ACCESSION   DBNGVD010000369 DBNGVD010000000
VERSION     DBNGVD010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1908239"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16CM_09960"
     CDS             <1..228
                     /locus_tag="AC16CM_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3140196.1"
                     /translation="DAGWIGEAGPGPSYLDPGSGGPENDFTNRNTTFMTWNLMHLARM
                     LKDAGGIPAHGNQRSEWVAGCRPDSQNPEHR"
     gene            complement(268..558)
                     /locus_tag="AC16CM_09965"
     CDS             complement(268..558)
                     /locus_tag="AC16CM_09965"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3140197.1"
                     /translation="MFYRQPARRASYFLVTSPCAVKEPRAVMREFGFEPPEGVEVVVY
                     DSTADIRYMVLPMRPSGTEDLGEEDLARLVTRDSLVGTSVPQAPNHPIPADA"
     gene            822..1238
                     /locus_tag="AC16CM_09970"
     CDS             822..1238
                     /locus_tag="AC16CM_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR3140198.1"
                     /translation="MALAFFRLQRVKSVTDKTRAFNKRFGNLLMMRLAGRRFFFAGVI
                     RHTGRRSGREYATPIWAVPTGEGVVISLPFGEGADWLKNVRAAGRATIETRGRTWMVA
                     DPEVVDKETAWPSLPRRARLLFGLAGIERYLQLSRL"
     gene            complement(1290..1676)
                     /locus_tag="AC16CM_09975"
     CDS             complement(1290..1676)
                     /locus_tag="AC16CM_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140199.1"
                     /translation="MYFGYLVAWFGIVVLGLVNATIRQIAYARYVGELAAHQISTLMF
                     AVLVGLYAWALSGFLKPSSPGEAIGVGIMWMVLTVAFEFALGRLVVGDPWGKLLGDYN
                     LLEGRVWGLFILWVGLAPYVFYRMKA"
     gene            complement(1707..2519)
                     /locus_tag="AC16CM_09980"
     CDS             complement(1707..2519)
                     /locus_tag="AC16CM_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3140200.1"
                     /translation="MSTTTAVQSNRLAVSLAVGLYLVWVLATYLLEGRIHTFLRPEAM
                     GARLTYALVANVLIGIGGSALVIRFLSRAGTLSTEQAGFRGLGHAAIAVAIGVALGFA
                     LYALQGAPSWNPMVLINAYSQVLVGSIAEILVCWAVVGSVSQVLLQDRGRWISLILAA
                     IIASVFFGVYHFGHSPPFNTVPTVLFLSVIGLVTSGFFFASRDVYGTIAFHNFLGILG
                     VLRALDSSGNLSAFEHPIIPLWIMAVVAVALLIGAQILLLDSGAAPTPPRVR"
     gene            complement(2827..3453)
                     /gene="nthA"
                     /locus_tag="AC16CM_09985"
     CDS             complement(2827..3453)
                     /gene="nthA"
                     /locus_tag="AC16CM_09985"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3140201.1"
                     /translation="MSDNHPHEATTTSPYAARIRAIEALLLEKSVLTEPEVQDKISYM
                     EARSPANGAKLVARAWVDDDFRELLLSDGKAAASALNIDPAHPAEFVVVENTPKIHNL
                     IVCTLCSCYPRSILGRPPDWYKSFEYRSRAVKEPRAVMREFGFETPEGVEVVVYDSTA
                     DVRYMVLPMRPSGTEDLDEEDLARLVTRDCLVGANVPRAPIHPVPTDN"
     gene            complement(3450..3743)
                     /locus_tag="AC16CM_09990"
     CDS             complement(3450..3743)
                     /locus_tag="AC16CM_09990"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019953876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HIR3140202.1"
                     /translation="MTYRPGDRVRILPSKKPGHVRTPGYLKGKSGWIEATIGPFPNPE
                     DLAYGHSGLPARNLYRVAFRQEDLWEEYEGPAEDILYADVYEHWLEPGEGGTA"
     gene            complement(3740..4039)
                     /locus_tag="AC16CM_09995"
     CDS             complement(3740..4039)
                     /locus_tag="AC16CM_09995"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140203.1"
                     /translation="MSGVHDMGGSFDGPVERDQHEVEDWERLADAISSALGAKGLYST
                     DEHRRAIESLKNYRDLSYYERWAAATEILLVEKNVLAVDEIDEKAAEIERRWGGA"
     gene            complement(4050..5036)
                     /locus_tag="AC16CM_10000"
     CDS             complement(4050..5036)
                     /locus_tag="AC16CM_10000"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR3140204.1"
                     /translation="MDAVEWFKHRSYDYKQFSDLEALAHRKRELGLTVSAVLPCRNVA
                     DTVGGIIDEIHAVNRQTGDHPLVDQIIAVDADSPDGTADVAAAAGAEVYSENELMSNH
                     GGAHGKGDAMWRALSVARGDLVMYLDADTKDFRPQLVYGILGPILEVPEVRYVKAAYR
                     RPFKSHETVELDGGGRVTEISTKPLFNLFYPELTGFVQPLAGEFVADRELFSSIPFLT
                     GYAVETGIMIDVLKMIGLDAMAQVDLGTRQNRHQPLRELSRMSYSVLRAVARRMRQDG
                     RLNQAQDPGSPDSLYQLSDYLHAVATPDGLKLQEYVEELVERPPIKEVLRVR"
     gene            5260..>5443
                     /locus_tag="AC16CM_10005"
     CDS             5260..>5443
                     /locus_tag="AC16CM_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014864982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3140205.1"
                     /translation="MRIVLTNDDGIEAPGLLAARRALEQVGEVITVAPDRNRSGVGRS
                     ISFGRSLHVEEVEMEDG"
BASE COUNT          999 a         1664 c         1779 g         1001 t
ORIGIN      
        1 gacgcgggtt ggatcgggga ggccggtccg gggccatcgt atctagatcc cggctccggg
       61 ggcccggaaa acgacttcac gaacaggaac accacattca tgacctggaa cctcatgcac
      121 ctggcaagga tgctaaagga tgccggtggc atcccggcac acggaaacca gaggtcggag
      181 tgggtagccg gctgccgtcc ggactcccag aacccggagc atcgctagcc ctcttctcgc
      241 tagcggcttc ccgagtacgc tactagctca ggcgtccgcc gggatcggat ggttcggcgc
      301 ctgtggcacg ctggtcccga ccagggagtc gcgcgttacg agccgggcca ggtcctcttc
      361 acccagatct tctgtacctg agggacgcat ggggaggacc atgtagcgga tgtcggcggt
      421 gctgtcgtaa acgacgacct cgaccccctc cggtggctcg aagccgaact cgcgcatcac
      481 ggcgcgcggc tctttgacgg cgcagggtga ggtgaccagg aagtagctgg cacgcctggc
      541 cggctggcgg tagaagatgt ctcgctcgcg agattcctgc cagcaaaagg gttagcgaca
      601 tccctcctca gatccataac aacggaacag agcagaatcg tgcgtgcgaa gctcggagaa
      661 tttaccttcc acgcacttcg aagaatagtc gtccagggta agatgggcgc aggagaaggg
      721 agacactcct ggtggcgagc gcgaacggta gacatgctag acgcagacta ttgaagcgtc
      781 tcctcctgcg actcggtctt ctcgcgccga tattatttgg ggtggccttg gcgttcttcc
      841 gcctgcaaag agtaaaatcc gtaaccgaca agacgcgcgc cttcaacaag cgcttcggca
      901 acctgctcat gatgaggctt gcggggcgtc gcttcttctt cgccggcgtc atccgccaca
      961 ccggacgccg ctcgggaagg gagtacgcca ctcctatatg ggcggtgccg acgggcgaag
     1021 gggtagtgat atccttgccg ttcggggagg gtgcggactg gctcaagaac gtgcgggccg
     1081 caggacgcgc gacgatcgag accaggggcc ggacctggat ggtcgcagac cccgaagtgg
     1141 tcgataaaga gaccgcgtgg ccctcgttgc cgaggcgggc gcggctcttg ttcggcctgg
     1201 ccggtataga gaggtacttg cagctgagca ggctctagga gcccgcctcg cggaaggttc
     1261 ggacacctac tagtagtggt ggtcggatgt cacgccttca tcctgtagaa aacgtagggt
     1321 gccagcccta cccacaggat gaacaggccc cacaccctgc cctccaggag gttgtagtca
     1381 cccaggagct tgccccatgg gtctcccact accaggcggc ccagggcgaa ctcgaaggcg
     1441 acggtcagga ccatccacat gatccccacc ccgatcgcct cgccagggga cgagggtttc
     1501 aggaacccgc tcagggccca ggcgtagaga ccgaccagga cggcgaacat aagggtggag
     1561 atctggtggg ctgccagctc gcccacgtac ctggcgtagg ctatctggcg tatggtggcg
     1621 ttgacgagcc ccaagacgac gataccgaac caagctacga ggtacccgaa gtacacgcta
     1681 cccccaacga gggttcgaca tccgagctac ctcacgcgcg gaggggtggg cgcggctccc
     1741 gagtcgagca acaggatctg cgcgccgatc aggagggcca cggccaccac ggccattatc
     1801 cacagcggga ttatcggatg ctcgaaggcc gagaggttgc ccgaggagtc gagcgcccgt
     1861 agcacgccca ggatccctag gaagttgtgg aaggcgatgg tgccgtagac gtccctggag
     1921 gcgaagaaga acccgctcgt caccaacccg atcacgctca agaacaagac cgtgggcacg
     1981 gtgttgaacg gcgggctgtg gccgaagtgg tagaccccga agaacacgct ggctatgatt
     2041 gcggctagga tcagagagat ccacctgccg cgatcttgca acaggacctg ggaaacgctc
     2101 cctaccacgg cccagcacac caggatctcc gctatggagc caaccagcac ctgcgagtag
     2161 gcgttgatga ggaccatcgg gttccaagag ggcgcaccct gcaaagcgta aagggcgaat
     2221 ccgagtgcca ccccgatcgc tacggcgatg gccgcgtgcc cgagtcccct gaagcccgcc
     2281 tgctcggtcg atagggttcc cgccctggag aggaagcgga ttaccagggc tgagccgcct
     2341 atccctatga ggacgttggc cacgagggcg taggtaagcc tcgcgcccat ggcctccggg
     2401 cgcaagaagg tgtggatgcg tccctccaag aggtaggtgg caagaaccca caccaggtaa
     2461 aggccgaccg cgaggctgac cgccaaccgg ttgctctgta cggccgtggt tgtgctcatc
     2521 gcgctacctc ctcctcgccg gctactcgcg gagcgccacg gcgctgtgcc gcacctagaa
     2581 ggctaccatt gcccctctcg agcgccttga gtaagaagcg ccagatgact ggtcaaaact
     2641 tgccaacccg atggtcatcg aagggcgaac ttctcggaac gccgtaaagc tggaggtcgt
     2701 agaaggattc ggatttacgg attgtgagcg tctgctggag aagggagatc ccctcctccg
     2761 tgcgactatg aacagcctca tgacgcggag gttggctttc tcgggaggag ctttatgaac
     2821 ggtttgtcag ttgtccgtag ggaccggatg aatcggtgcc cgcggcacgt tggctccgac
     2881 caggcagtct cgcgtcacga gccgggctag atcttcttca tccagatctt cggtgcctga
     2941 ggggcgcatg gggaggacca tgtagcggac gtcggcggtg ctgtcgtaaa cgacgacctc
     3001 gacgccctcc ggggtctcga agccgaactc gcgcatcacg gcgcgcggct ctttgacggc
     3061 gcgggagcgg tattcaaagc tcttgtacca gtcgggcggg cggccgagga tggaccgcgg
     3121 gtagcaggag cagagtgtgc acacgatcag gttgtgtatc ttcggcgtgt tctctacgac
     3181 gacgaactcg gctggatgcg cggggtcaat gttcagcgcc gaggccgcgg ccttgccgtc
     3241 ggagaggagc agttctctga agtcgtcatc tacccaggcg cgggctacga gcttcgcgcc
     3301 gttggcaggg gagcgggcct ccatatagga gatcttgtct tgcacctcgg gctcggtaag
     3361 aacacttttc tccaaaagga gcgcctcgat ggccctgatg cgggcagcgt atgggctcgt
     3421 ggtcgtcgct tcgtgcgggt gattatcgct cacgccgtgc ctccttcgcc gggctcgagc
     3481 cagtgttcgt acacgtcggc gtacaggatg tcttctgcgg gaccctcgta ttcttcccag
     3541 aggtcctcct gcctgaaggc gaccctgtac aggtttcgcg cgggcaggcc ggagtggccg
     3601 taagcgaggt cctcggggtt gggaaacggc ccgatagtag cctcgatcca gcccgattta
     3661 cctttgagat atccgggggt acgaacgtgg ccaggtttct tcgatggcaa gatcctgacg
     3721 cggtcgcctg gcctgtaggt cacgcccctc cccagcgacg ttctatctcg gccgccttct
     3781 cgtctatctc gtcgaccgca aggacgttct tctcgacgag gagtatttcg gtggccgcgg
     3841 cccagcgttc ataataggag aggtcgcggt aattctttag gctctcaatc gccctgcggt
     3901 gttcgtccgt gctgtagagg cccttcgcgc cgagggcgga gctgatggcg tcggcgaggc
     3961 gttcccagtc ctcgacctcg tgctggtccc tctctacggg gccgtcgaaa gagccgccca
     4021 tgtcgtggac accgctcaag gctgcgcccc tatctgaccc tcaacacttc tttgatcggg
     4081 ggtctctcca cgagttcttc gacgtactcc tggagcttga ggccgtccgg cgtcgcgacg
     4141 gcgtgcaggt agtcggagag ctggtagagg gagtccggcg agcccgggtc ctgggcctgg
     4201 ttcaggcgtc cgtcctggcg catgcgcctc gcgaccgccc gcagcaccga gtaggacatg
     4261 cgggagagct cgcgcagggg ctggtgacgg ttctggcggg tgccgaggtc cacctgcgcc
     4321 atggcgtcga gcccgatcat cttcaacacg tctatcatga tcccggtctc caccgcgtag
     4381 ccggtgagga atgggatgga ggagaagagc tccctgtcgg ccacgaactc gccggcgagc
     4441 ggctgcacga agcctgttag ctcggggtag aagaggttga agagcggctt ggtcgagatc
     4501 tccgtgaccc tgccgccgcc gtccagctcc accgtctcgt ggctcttgaa ggggcgcctg
     4561 taggctgcct tgacgtagcg cacctcgggc acctctagta tggggccgag gatgccgtag
     4621 acgagctgcg gcctgaagtc cttggtgtcg gcgtccaggt acatcacgag gtcgccgcgg
     4681 gccaccgaga gggcgcgcca catggcgtcg cccttgccgt gcgcgccacc atggttgctc
     4741 atcagctcgt tctccgagta gacctcggcg cctgcggcgg cggccacgtc tgccgtgccg
     4801 tccggcgagt ccgcgtcgac ggcaatgatc tggtcgacca gcggatgatc gcccgtctgc
     4861 cggtttacgg cgtggatctc gtctatgatc ccgcccaccg tgtcggcaac gttgcggcag
     4921 ggcaacaccg cgcttaccgt gaggcctagc tcgcgcttgc ggtgggctag agcctcgagg
     4981 tcggagaatt gcttgtagtc gtaggagcga tgcttaaacc actctacagc gtccacgggc
     5041 atcacactcc tcacgttcgc ttccacatca cagccccggt aagctaccgt acatcacccg
     5101 atttgtccat tcttattcct taacatgtga cgaacgtccc attctcgtgc cgcatcatcc
     5161 cgtctccctt ctcgccgccg gggcctccgg agcgggtggt agaatcagga gcctttgggg
     5221 agaaccacgg cctcctgacg gacggagagt ttggacgtta tgcgcatagt attgaccaac
     5281 gacgacggca tcgaggcccc tgggctcctg gcggcccgac gggctctgga gcaggtgggc
     5341 gaggtgatca ccgttgcccc cgacaggaac cgttccggcg tgggcaggag catctccttc
     5401 gggcgctcgc tccacgtcga agaggtcgag atggaagatg ggg
//
LOCUS       DBNGVD010000370         2888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1363195, whole genome shotgun sequence.
ACCESSION   DBNGVD010000370 DBNGVD010000000
VERSION     DBNGVD010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1363195"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..869
                     /locus_tag="AC16CM_10010"
     CDS             168..869
                     /locus_tag="AC16CM_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140206.1"
                     /translation="MIHPALLLLQNLTEEPEFIDWEWLRENFWEDIVPAIQGHIFLSF
                     VSVAIALVIALPVGILSARFRKIYPPVTGVTGILYSIPSLALFAILISIPFVPLGPVP
                     VIIALVAYSLLILIRNTVAGLDAVPPETVDAARGMGLTGRQVLFRVELPLALPIIVAG
                     IRIATVTIIGIATIGAYINGGGLGKLIFDGINRQFPTMIIAGAVLATALAILADLLLL
                     GLERYLRPWARARRT"
     gene            871..1524
                     /locus_tag="AC16CM_10015"
     CDS             871..1524
                     /locus_tag="AC16CM_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140207.1"
                     /translation="MFSEIFEVFVRPGFLTLLREHVVLSVVALLIAVAVALPVAVLVR
                     NTRVGATIAINVGNVGRAVPSLAILALALPFLGLGFNTALLALTALAIPPILINASTG
                     LRQVDKGIIDAARGMGLSGNQILTGIQLPIAAPVIFAGIRTSAVQTVASATLATFVGA
                     GGLGDLIVEGLQRRGPEILLAGAFSVAVLAIITEVGFGLLERAFTPKGLKIAQKRGR"
     gene            1550..2503
                     /locus_tag="AC16CM_10020"
     CDS             1550..2503
                     /locus_tag="AC16CM_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3140208.1"
                     /translation="MKNKTKLIRAVPAMLAGLSLLLVVACGNVGGSGESDSGGGPTIT
                     VGSKNFTEQYILGNMYAQALEANGFNAETKLDLGSEQIADKALQSGQIDLYPEYTGTA
                     LVAIVPFKGNPSDLNSPQATYEAAKKGYADRSPADTMLTPAPFNNTYGIFVRKSVAKE
                     MNLTNMEQLSKASKNLTFISYSEFQNRSDGYPNVKKNYPAFDFEQIEIVNQLGLRYSG
                     IENGDGDVGVGFTTDGQLTSPQLVVMKDEKAIWPFYYPAPVVRTDVLEKHPKIKGILN
                     EVSASLDVEKMRELNGQVDLDQEDPEDVAAEYLEQEGIVKN"
     gene            complement(2623..2862)
                     /locus_tag="AC16CM_10025"
     CDS             complement(2623..2862)
                     /locus_tag="AC16CM_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140209.1"
                     /translation="MSETTYNRIAMLRAERGISRRQLAEALGVHYQTVGYLERGEYSP
                     SLYLALKIAEYFEVPVEIIFSTSPFPRIGSTERSA"
BASE COUNT          557 a          916 c          881 g          534 t
ORIGIN      
        1 gtcagggacg cgctctccca gctgatcggg tcggggcaca ccaccgcggt gatcgagaag
       61 gacgggccga acggcctgtt gaccttcgac gccatagagg ggctcatggc cgacgcgtcg
      121 accgatacgg gtgacacaga gcgggctagc ggcggggaac catcggcgtg atccaccctg
      181 cgctgctcct cttgcagaac ctgaccgagg aaccggagtt catcgactgg gagtggctca
      241 gagagaactt ctgggaggat atcgtgccgg ccatacaggg gcatatcttt ctctctttcg
      301 tgtccgtcgc gatagcgctc gtcatcgcgc ttcccgtggg aatcctctcg gcacgctttc
      361 gcaagatcta cccgccggtc accggtgtca cgggcatcct ctactctatt ccgagcctcg
      421 cgctcttcgc catcctcatc tccataccct tcgtgccgct cggacccgta cccgtgatca
      481 tcgccctcgt ggcatattcg ctcttgatcc tgatccggaa caccgtcgcc ggactggatg
      541 ccgtgccgcc cgagaccgta gacgcggccc gcggcatggg cctgacgggc cgccaggtac
      601 tgttccgggt cgagttgccg ctggcgttgc ccataatcgt agccggcatc cgcatcgcca
      661 ccgtgacgat catcggcatc gccaccatcg gcgcctacat caacggcggc ggtctgggaa
      721 agctgatctt tgacggcatc aaccgccagt tcccgaccat gatcatagcc ggagcggtac
      781 tcgcgacggc gctagccatc ctcgcggacc tcttgcttct cggactcgaa cgctacctga
      841 ggccttgggc gcgggcgagg aggacgtagc atgttctccg agatattcga ggtcttcgtc
      901 cgtccaggtt tcctcaccct gctcagggag cacgtcgtgc tctcggtggt agcactgctc
      961 atcgccgtcg cggtggcttt gcccgttgct gtgctcgtac gcaacacgcg ggtcggcgcg
     1021 acgatagcca tcaacgttgg caacgtcgga cgcgccgtgc cgagcctggc gatactcgcg
     1081 ctcgccttgc cgtttctggg cctcggtttc aatacggcgc tgttggcgct cacggcgctg
     1141 gccataccgc ctatcctgat caacgcctcg acgggtctca ggcaggtcga taaaggcatc
     1201 atagacgccg cccggggtat ggggttatcc gggaaccaga tcctgaccgg catccagcta
     1261 cctatagccg cgccagttat cttcgccgga atccgtacct ccgccgtgca gaccgtcgcc
     1321 agcgccaccc tcgcgacgtt cgtgggggct ggtggactgg gggacctgat cgtcgagggc
     1381 ctccaacgcc gcgggccgga gatactgctc gccggggctt tctccgtcgc ggttctcgcg
     1441 ataatcaccg aggtcggctt cggccttctt gagagggcgt tcaccccgaa agggctcaag
     1501 atcgcgcaga agcgaggtag atgatgaggc caactacaga ggagggaaga tgaagaacaa
     1561 gaccaagcta atccgcgccg tgccagcgat gctggcgggc ctatcgctgt tgctcgtcgt
     1621 tgcttgcggc aacgtgggtg gcagtggcga gtcggactcg ggcggcggcc cgaccataac
     1681 ggtgggatcg aagaacttca ccgaacagta catcctgggc aacatgtacg cccaggcgct
     1741 ggaggccaac gggttcaacg ccgagaccaa gctggatctg ggctccgagc agatagcgga
     1801 caaggcgcta cagagcggcc agatcgacct ctaccccgag tacacgggca ccgccctggt
     1861 ggctatcgtt cccttcaagg ggaacccgtc tgatctgaat tcgccccagg cgacctacga
     1921 ggcggccaag aagggctacg ccgatcgtag tcccgccgac acgatgctga cgccagcgcc
     1981 tttcaacaac acctacggca tcttcgtccg caagtcggtg gccaaagaga tgaacctcac
     2041 caacatggag cagctctcca aggcctccaa gaacctcacg ttcatctcct actccgagtt
     2101 ccagaatcgc tctgacggct accccaacgt aaagaagaac tacccggcgt tcgacttcga
     2161 gcagatcgag atcgtcaacc agctgggcct gcgctactcc gggatcgaga acggtgacgg
     2221 cgacgtcggg gtcgggttca ccaccgacgg ccagcttacc tcaccgcaac tcgtggtgat
     2281 gaaggacgag aaggccatct ggcccttcta ctacccggca ccggtcgtca gaaccgacgt
     2341 gctagagaaa cacccgaaga taaaaggcat cctgaacgag gtctcggcaa gcctcgacgt
     2401 ggagaagatg cgcgagctga acgggcaggt cgacctcgac caggaggacc ctgaagacgt
     2461 ggcagccgag tacctggagc aggaaggcat agtcaagaat tagagctgtt ggccaggagt
     2521 tgaactcctg gccagctatc agccatcagc ggtcagccat cagcaaaaaa gctgaaagct
     2581 gaccgctaaa tgctgaaggc gcggttcgct tcgcgaaccg ctctaagcgg agcgttcggt
     2641 gcttccgatg cggggaaatg ggctggtgga gaagatgatc tccacgggga cctcgaagta
     2701 ctcggcgatc ttcagggcga ggtacaggct ggggctatac tctccccttt cgaggtagcc
     2761 cacggtctgg tagtggacgc ccagggcttc ggccagttgc cgccgtgaga tgcccctctc
     2821 ggcgcgcaac atggctatgc ggttataggt ggtctcgctc accagccggc ccgctgcatc
     2881 gctttctc
//
LOCUS       DBNGVD010000371         4161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4823629, whole genome shotgun sequence.
ACCESSION   DBNGVD010000371 DBNGVD010000000
VERSION     DBNGVD010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4823629"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC16CM_10030"
     CDS             <1..184
                     /locus_tag="AC16CM_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140210.1"
                     /translation="PEEWGAGPVVELEGFVLDPVGKARAFLENGPQESMAFYVQFTLE
                     LLEGESGDGIGDPRSA"
     gene            complement(264..512)
                     /locus_tag="AC16CM_10035"
     CDS             complement(264..512)
                     /locus_tag="AC16CM_10035"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3140211.1"
                     /translation="MEATREKTSIKLYTGSYCPFCRRVTKELDRLELDYEAVNADADG
                     RDEVIRLSGQRAIPILTIGDEVLVDSSHIIRELRSRYA"
     gene            complement(594..1067)
                     /locus_tag="AC16CM_10040"
     CDS             complement(594..1067)
                     /locus_tag="AC16CM_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3140212.1"
                     /translation="MVPSPETRLRELGIDLPAAPAPAGAYVPATRTGNLVFTAGQLPL
                     EGGELTIRGKVGDTVGVEEAREAARLCATNALAAASTQTGGIAGISRVLKVTGFVASA
                     PGFNAQPQVLNGASEFLGEVFGDAGLHARSAVGVAELPMDAPVEVEVVVEAHPGA"
     gene            complement(1068..1562)
                     /locus_tag="AC16CM_10045"
     CDS             complement(1068..1562)
                     /locus_tag="AC16CM_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3140213.1"
                     /translation="MDGDIQDRNSILVTLRLLTHGVYVLGTRRGRQSNAMTASWVMQT
                     SERPPCLAVAVRGDRYTHDIMLESGTFALSVLRDDQVDVATHFADTSGEYHDKLLGIP
                     YGLTPGGSPFLLDCLAYLDCKVIDRARAGDHTVVVGEVTASDNLDVSYPLIYDPSEYE
                     EALR"
     gene            1646..2629
                     /locus_tag="AC16CM_10050"
     CDS             1646..2629
                     /locus_tag="AC16CM_10050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR3140214.1"
                     /translation="MSGALFGIGLLQAVIYLLFVRAIDLYEREPLRYVLPVFVWGFAV
                     ATTVSLIFNTLFQITLSSVTSVQTASFFTAVISAPVVEESSKGLALLIIFFIAYLLRR
                     RTGTIEFSGVMDGIVYGSAVGFGFAIAEDLLYGLQYGPETFVVRRIFGGFAHASFTAL
                     TGIGIGLIPWVQNRALKVLVPALGLIGAMALHATFNFLATVFGPVAYLFLFLVILGYI
                     VVIIVCLYIERRIIRDELGDEVGAGTITAQEYAILPSYFRRKAYYVGLVFTGRLREWR
                     AARKVHHFAVDLALAKRLARRSGSPGSMDRVGTLRQKILGARGTNKVRMVS"
     gene            2647..3222
                     /gene="mobA"
                     /locus_tag="AC16CM_10055"
     CDS             2647..3222
                     /gene="mobA"
                     /locus_tag="AC16CM_10055"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR3140215.1"
                     /translation="MILAGGRSRRMGREKLLLEIGGVPLIERVRNTVSASCEEVILVG
                     GEVTGLQGVRHVSDGRSGRQGPLAGIEAGLAASRNARVFVAAGDMPFLSPDLIRYLLG
                     RLGREGNVAVIPRHGGRRHPLCGAYARALLPRVGAALDGGVRAVGAFLDGAVGVEYVE
                     EELRRYGMPDLLLMNVNTPGDLEAARREAER"
     gene            3219..3791
                     /locus_tag="AC16CM_10060"
     CDS             3219..3791
                     /locus_tag="AC16CM_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3140216.1"
                     /translation="MSILGELGELLRMYAGRTSATGGRRPRVAVILGAQVLRGGRASP
                     TLAARTRHAGSMFARGEVDLLIPTGGVGDHPPAEADVMSVILLDMGVPETAILRERFA
                     ASTWQSAVEVARISRREGIGEVLAVSDPLHCVRAVSAFEKVGLVAVSEPVYGSPMWSK
                     KWLRRGQLAREGGALVWYWIRYGVGRTSRR"
     gene            3875..>4161
                     /locus_tag="AC16CM_10065"
     CDS             3875..>4161
                     /locus_tag="AC16CM_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140217.1"
                     /translation="MDGQRATNSGPISTGLRYGIFGVLVALASDLYFLVIPPESLPGW
                     ILAVIAQYRTRMALALFLFLAVLAALRVQPTRLDPGVPYRSLLVRDGALAGT"
BASE COUNT          667 a         1287 c         1386 g          821 t
ORIGIN      
        1 acccgaggag tggggggccg ggcccgttgt cgagcttgag ggtttcgtgc tcgaccccgt
       61 tggaaaggca agggctttcc tcgagaacgg cccgcaagaa tctatggcct tctacgttca
      121 gttcacgctg gaactgcttg agggtgaatc gggggatggt atcggcgacc cccggagcgc
      181 ctgaagcggt tcgttatcag cttctccggc taaccgccga tggacaaagt ctcgatgtgg
      241 gcgactaact cgcccacacc gcactaggcg tagcggctgc gcagctcgcg gataatgtgc
      301 gaggaatcga cgagcacctc gtccccgatg gtcaggatcg ggatcgcgcg ctgaccggag
      361 agccgaatga cctcgtcccg gccgtcggca tccgcgttga ccgcctcgta atccagctcc
      421 agccggtcga gctctttcgt gacccgccgg cagaacgggc agtaagaacc cgtgtatagc
      481 tttatggacg tcttctcacg agttgcttcc atggtaaccc tcccagactc tatacgataa
      541 taaatctgtt tacacggata ttttataccc gggaggcgga aattgtaacc cggtcaggct
      601 cctgggtgtg cctccaccac gacctcgacc tcgacggggg cgtccatagg gagttcggcg
      661 acgccgacgg cggagcgggc gtgcaggccc gcgtcgccga atacctcgcc cagaaactcc
      721 gaggcgccgt tcaagacctg cggctgagcg ttgaagcccg gggcggaggc gacgaagccc
      781 gtcaccttga ggacgcgcga gatcccggct atgccgcccg tctgggtgga ggcggcggcg
      841 agggcgttcg tggcgcacag tcgggccgcc tcgcgagctt cttcgacccc cacggtatct
      901 cccaccttgc cccgaatggt tagctcgccc ccttcgaggg ggagttggcc cgcggtgaag
      961 accaggttgc ctgttcgcgt ggccggtacg taggcaccgg ccggtgccgg ggctgccgga
     1021 aggtcgatgc caagttctcg caggcgcgtt tcggggctgg ggaccatcta gcggagggcc
     1081 tcttcgtatt cgctcgggtc gtagatcagg gggtagctca cgtcgaggtt gtcgctcgcc
     1141 gtcacctcgc cgaccaccac ggtatggtcg cccgcgcggg ccctgtcgat caccttgcag
     1201 tccaggtagg ccaggcagtc aaggaggaag ggcgagcccc cgggcgtcag gccgtagggg
     1261 ataccaagta gtttatcgtg gtactcgccg ctcgtgtccg cgaagtgcgt cgccacgtcg
     1321 acctgatcgt cgcgcagcac gctaagggcg aaggttccgc tctccagcat gatgtcgtgc
     1381 gtgtagcggt cgcccctcac cgccacggca aggcaaggtg gtcgttccga agtttgcatt
     1441 acccaggagg ccgtcatcgc gttcgactgg cggcccctgc gggtccccag aacgtacacg
     1501 ccatgggtca gaagacgcag ggtaacgagt atagagttgc ggtcttggat gtccccgtcc
     1561 aatccactcc ttccgattcg gacataggta ttctatcagg agcggttata ctgcggccca
     1621 tgtcttctgc gcgaaggaca ccaagttgag cggtgcccta ttcggcatcg gtctgctgca
     1681 ggccgtgatc tacctgctct tcgtacgcgc tatagatctc tacgagcgtg agcccctgcg
     1741 ttacgtgctt cccgtcttcg tgtggggttt cgcggtggcc accaccgtct cgctcatctt
     1801 caacacgctt ttccagatca cgctcagctc cgtgactagc gttcagacgg ccagcttctt
     1861 taccgcggtc atatcagcgc ccgtcgttga ggaatcctcc aagggtctgg ccctgcttat
     1921 catcttcttc atcgcttacc ttctgcgccg gaggaccggc acgatcgagt tctccggcgt
     1981 catggacggc atcgtctacg gctcggccgt gggtttcggg ttcgccatcg ccgaggacct
     2041 cctctacggg ctgcagtacg ggccggagac gttcgtcgta cgcaggatct tcggtggctt
     2101 cgcccacgcg tcgttcaccg ctctgaccgg gatcggcatc ggcctgatcc cctgggttca
     2161 gaacagggcc ctgaaggtac ttgtgcctgc gcttggcctc atcggggcca tggcgttgca
     2221 cgcgaccttc aacttcctgg ccaccgtgtt cggccccgtt gcctacctgt tcctgttcct
     2281 ggtgatactt ggctacatcg tggtcatcat cgtttgtctc tacatcgagc ggcgcatcat
     2341 tcgggatgag ctcggagacg aggtcggggc cggcaccata accgcgcagg agtacgccat
     2401 actcccgagc tacttccgac gcaaggccta ctacgtgggg cttgtcttta cggggagact
     2461 tcgcgagtgg cgtgcggcgc gcaaggtgca ccatttcgcg gtagacctgg ccctcgccaa
     2521 acgcctagcc cgccgttccg ggagccccgg cagcatggac cgggtcggga cgctccgcca
     2581 gaaaatcctc ggcgcgaggg ggacaaacaa ggtcaggatg gtctcctgag gcccacggcc
     2641 tcgggggtga tccttgccgg tggccgaagc cgtaggatgg gccgtgagaa gctgctcctc
     2701 gagataggtg gcgtgcccct catcgagcgc gtcagaaaca ccgtctcggc ctcttgcgag
     2761 gaggtaattc tcgttggtgg ggaggttaca ggcctgcaag gagtgcgcca cgtatccgac
     2821 ggcagatcgg ggaggcaggg accacttgcc gggatagagg ccggcctggc cgcctcgaga
     2881 aacgcccgcg tcttcgtcgc tgctggtgac atgccctttc tgtcccccga cctgatccga
     2941 tatctgttgg gccgcctcgg ccgcgaagga aacgtggcgg tgatcccacg gcacggaggt
     3001 aggagacatc cactgtgcgg cgcctacgcc agggctctcc tgccgcgggt gggtgcagct
     3061 ctcgacggcg gagtgcgggc cgtaggtgcg tttctggatg gggctgtcgg ggtcgagtat
     3121 gtagaggagg agttgcgacg gtacgggatg ccggatctgc ttctgatgaa cgtcaacaca
     3181 ccgggggacc tggaagcggc gaggcgtgag gcagaacggt gagcatcctc ggggagctcg
     3241 gagagctact cagaatgtac gcgggccgga cgtctgccac cggtggacgg cggccgcggg
     3301 tcgctgtgat cctcggcgcc caggtgctgc gcggcggaag ggcgagcccg acgctggcgg
     3361 ccaggacgcg ccacgccggc agcatgttcg cccgcggtga ggtcgacctt ctcataccca
     3421 ccggcggagt cggtgaccat ccgcccgctg aggcggacgt gatgtccgta atcctgctcg
     3481 acatgggcgt gcccgagacg gcgatcctgc gcgagcgttt cgcggcgagc acctggcaat
     3541 ctgccgtcga ggtggcccgg atctcacgcc gcgagggcat aggtgaggtc ctcgcggtca
     3601 gcgacccact gcactgcgtc agggcggtct ctgcttttga gaaagttggg cttgttgccg
     3661 tttcagagcc ggtctacggc agcccgatgt ggagcaagaa atggttgcgc aggggacaac
     3721 tggcaaggga gggcggggcc ctcgtgtggt actggatcag gtacggggta ggacggacct
     3781 cgcggcggta gccgcggtcc cggcggcccg cgctggttgc tcctgcagat cgtattcaca
     3841 cagcgacccc tggatgatgc tgtatgctcc ccgtatggac ggtcagaggg cgacaaattc
     3901 gggcccgata agcacgggct tgcgttacgg gatcttcggt gtgctggtcg cgctcgcttc
     3961 cgatctctat ttcctcgtga tccctccaga gagcctgccc ggttggatcc tggccgttat
     4021 cgcccagtac aggacgcgga tggccctcgc cctgtttctg tttctggccg ttctggcggc
     4081 cttgagggtg cagcccacgc ggctcgatcc tggtgtgccc taccgatccc tgctcgtgcg
     4141 cgacggcgcg ctcgcgggga c
//
LOCUS       DBNGVD010000372         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3002468, whole genome shotgun sequence.
ACCESSION   DBNGVD010000372 DBNGVD010000000
VERSION     DBNGVD010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3002468"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2575
                     /locus_tag="AC16CM_10070"
     CDS             <1..>2575
                     /locus_tag="AC16CM_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HIR3140218.1"
                     /translation="DTERSNWTYDETAGQYFWHRFFSHQPDLNHDNPQVFKAIMRVMR
                     FWLDAGVDGLRLDAIPYLIEREGTDNENLPETHEVLKKMRAEMDARYENRLFLAEANQ
                     WPEDVLPYFGDGDECHMAFHFPLMPRIFMALAQEDRHPIVEIMHQTPEIPEGCQWAIF
                     LRNHDELTLEMVTDRERDYMYQIYATDPRMRINVGIRRRLASMMENDRPRIELLNSLL
                     MSMPGTPIIYYGDEIGMGDNIFLGDRDAVRTPMQWSSDRNAGFSRAEPARLYLPPIMD
                     PVHSYEAVNVEAQGRSAGSLLNWMKRLIAVRKAHRAFGRGTLEFLHPGNRKVIAYLRE
                     HEDEAILCVANLSRSAQPVELDLSRFRGLVPVELLGNSSFPPLGDLPYFLTLPGYGFY
                     WFRLAREVAAPRWHQESPSYAEPPVLVLPAGRQTARSADRFPLRLPTRQIGQLEREAL
                     PNFLGSRRWFAAKGDGIERVEVLAQASLADDMLLTLLEVHLGNEASEGAEPQIYFLPL
                     AAAWEEGTDGDGVSVYMPDVLTKLRRRARVGVLLDALSDEAFCRSVVAAMGENTGIKI
                     GEGEIHFSATGVFGDLIGDGPVEELSVRRAEVEQTNSSVILGERLILKAYRRLQKGTN
                     PDLEIGRYLTEETRFENTPPLAGAIEYTGPDGETTTLGLLQGYVENQGDGWSYVLDYL
                     DRYLEDRLLTSWSPEGEDAPDEELAPEDTFFASLVRILGLRTGELHAAFAAPTDDEAF
                     APETAPPEEVAGWVDGVLAEAGSTLDLLKRRRSSLPDEVAPDVERLLGLREELRRSVK
                     SVAGQNFGVVKTRHHGDYHLGQVLVVSNDFEIIDFEGEPARPLAERRKKHSPLRDVAG
                     ML"
BASE COUNT          488 a          837 c          854 g          396 t
ORIGIN      
        1 gataccgaga ggtccaactg gacgtacgac gagaccgcgg gccagtactt ctggcaccgc
       61 ttcttctccc accagcccga cctcaaccac gacaacccgc aggtcttcaa ggcgataatg
      121 cgtgtgatgc gcttctggct cgacgccgga gtggacggcc tcaggctcga cgccattccg
      181 tacctgatcg agcgtgaggg cacggataac gagaaccttc cggagacgca cgaggtcctg
      241 aagaagatgc gggcggagat ggatgcgcgc tacgagaacc gcctctttct ggccgaggca
      301 aaccagtggc ccgaggacgt cttgccgtac ttcggggacg gggacgagtg ccacatggcg
      361 ttccacttcc cgctcatgcc acgcatcttc atggcgctcg cgcaggagga caggcatccc
      421 atcgtggaga tcatgcacca gacgccggag ataccggagg gttgccagtg ggccatcttc
      481 ctgcgcaacc acgacgagct taccctggag atggtcaccg acagggaacg cgactacatg
      541 taccagatct acgcgaccga cccacgtatg cgcataaacg tcggcatccg ccgccgactc
      601 gcctccatga tggagaacga caggccccgc atcgagctcc tgaacagcct gctcatgagc
      661 atgccgggta cgcccatcat ctactacggg gacgagatag gcatgggcga caacatcttt
      721 ctaggggaca gggacgccgt caggacgccg atgcagtggt cgagcgacag gaacgccggc
      781 ttctcccggg ccgagcccgc acggttgtac ctgccgccta tcatggaccc cgtacacagc
      841 tacgaggccg tcaacgttga ggcccagggc cgcagcgccg gctcgctcct gaactggatg
      901 aagcgactta tagccgtacg caaggcgcac agggccttcg ggaggggaac gctggagttt
      961 ctccaccccg gcaacaggaa ggtcatagcc tacctcaggg agcacgagga tgaggccatc
     1021 ctgtgcgtgg ccaacctgag ccgctctgcc caacccgtgg agctcgacct ctcgcgcttc
     1081 aggggcctgg tccccgtcga gttgctcggc aacagcagct ttcccccgct gggggatctg
     1141 ccctactttc tcaccctgcc ggggtacggt ttctactggt tccgcctggc gcgggaggtc
     1201 gcggccccga ggtggcacca ggagagcccg tcgtacgccg agccgcccgt gctggttctc
     1261 cccgccggca ggcagacggc ccgcagcgcg gacaggttcc ccctgcgcct cccgacgcgt
     1321 cagataggcc agctagagag ggaggccctt ccgaacttcc tcggctcccg caggtggttc
     1381 gccgccaagg gcgacggcat cgaacgcgtc gaggtcctgg cgcaggcttc gctcgccgac
     1441 gacatgctac tcaccttgct cgaggtacac ctgggcaacg aggcctccga aggggcagaa
     1501 ccccagatct acttcctccc gctcgcggcg gcctgggagg aaggcacgga cggggacggc
     1561 gtctcggtgt acatgcccga cgtgctgacc aagctcaggc ggcgcgccag ggttggggtg
     1621 ctgctcgacg cgctctccga cgaggcattc tgccgcagcg tcgtcgcggc gatgggggag
     1681 aacaccggga taaagatcgg ggagggcgag atccacttct cggcgaccgg tgtgtttggg
     1741 gatctcatcg gtgacggacc ggtcgaggag ctaagcgtga ggcgggccga ggtggagcag
     1801 acaaacagct cggtaatact gggagaacga ttgatcctga aggcgtacag gcgcctccag
     1861 aagggcacga accccgacct ggagatcggc cgctacctga ccgaggagac ccgtttcgag
     1921 aacacgccgc cgctggccgg cgcgatcgag tacacgggcc cagacgggga gacgacaacc
     1981 ctcggcctcc tgcaaggcta cgtggagaac cagggcgacg gctggtccta cgtgctcgac
     2041 tacctcgacc gttacctgga agaccgcctg ctgacctcgt ggagcccgga gggtgaggac
     2101 gcgccggatg aagagctggc gcccgaagac accttcttcg ccagcctggt tcgtattctc
     2161 ggcctgcgca cgggagagct tcatgccgcc ttcgccgccc cgacggacga tgaggccttc
     2221 gcccccgaga ctgcaccccc cgaagaggtg gcggggtggg tcgacggggt cctcgcggaa
     2281 gcggggagca ccctcgacct gctgaagcgg cggaggagca gcctgcccga cgaagtcgcc
     2341 ccagacgtcg agcggctcct cggtctgcga gaggagttga ggaggagcgt aaagagcgtc
     2401 gccgggcaga atttcggggt cgtcaagacc aggcaccacg gcgactacca cctcgggcag
     2461 gtgctcgtgg tcagcaacga cttcgagatc atagacttcg agggcgagcc ggcgaggccc
     2521 cttgccgagc ggcgcaagaa gcactcgccc ctgcgggatg tggcgggtat gctcc
//
LOCUS       DBNGVD010000373         3530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1364180, whole genome shotgun sequence.
ACCESSION   DBNGVD010000373 DBNGVD010000000
VERSION     DBNGVD010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1364180"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16CM_10075"
     CDS             <1..390
                     /locus_tag="AC16CM_10075"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018829743.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3140219.1"
                     /translation="YEGPKGGPGMREMLGVTAALIGQGLGETVALITDGRFSGATRGL
                     MAGHVAPEAAHRGPIAALKDGDIITFDIDGRTLSVDLSDEEIEARLADWQEPEPRFAT
                     GVMAKYAALVSSASEGAVTDPNLGTTG"
     gene            complement(472..2841)
                     /locus_tag="AC16CM_10080"
     CDS             complement(472..2841)
                     /locus_tag="AC16CM_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140220.1"
                     /translation="MRGALTGAGVPFGALLRRHRATAGVTQEELAGRTGLTPQAIGLL
                     ERGKRRRPHSYTVQKLAEALELEGRELTEFEAAARRASIHRAPAATSVRTLPVPATPL
                     IGREREVSIAVELLLSDEVQLLTLTGTGGVGKTRLALEVAGRCSETFRDGATFVRLAP
                     VRDPDLVPSVIANELGIEDVGNRSMYQSLLQGLAGEQRLLVLDNFEHLPEAVPVVADL
                     LRECPELTVLATSRAPLRLSVERQFPVPPLSIDAEASSGRSEAVRLFEERARAVAPFF
                     APGPSGDAEVAEICRRLDGLPLAIELAAARVKIFAPHALLERLDHRLSLLSGGARDVP
                     ERHQTLRDTVAWSYDLLKPAEQALFGRLSVFAGGFTLEAAEAVCQTDEGSAFEILASL
                     VDNSLVVSNSADSGRPGDEEPRFTMLETIREYAAERLASDGEAERVHRAHAAYYLALA
                     EAAQPEVSQLMLGRWLEVLEREHENLRAALRWAIRSGDVDFGTRLALMMWRFWPERHH
                     VTEGRGWLEAILALGPPESGLSEPSLPARQWAFLHLVAGILAGGQGDYDRAVELYQES
                     LSLYRTMGHTRGTSGPLRELGVAAYHRGDYELAVSLSEQALAASRESDSAFGRGLAVC
                     TLADALRAKGEIERARSLLEESLASLRHETYPMRVANAVAITLARLGSIECELGRYVR
                     ARELHKESLALSRQYGFTFEVFFCLDGLARVEAINGRPERAARLLGTSEAVREEVGAR
                     LTRLTRSDHDHAAALARAALGDQAFETTWNRGHESALDETISEVLDTNG"
     gene            complement(2896..>3530)
                     /locus_tag="AC16CM_10085"
     CDS             complement(2896..>3530)
                     /locus_tag="AC16CM_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140221.1"
                     /translation="ARVAPHVAAGLKTAALRSRADEERLGPGIPGVIVLDRQGEVLSY
                     TPAAERLLSEVEDLGPGWERDAPIAVTMAAGALKRALAPDSDADLALVPRVRIRGRSG
                     RWLTLHASLTESTPGRESETVVVVSPSGPEEVARLNLDSYGLTGREAEIVRLAARGSS
                     TKEISETLFISQHTVNNHFRSIFEKTGVNSRRQMVQRLYLQQLIPGVLGE"
BASE COUNT          613 a         1243 c         1089 g          585 t
ORIGIN      
        1 tacgagggtc ccaagggcgg accgggcatg cgcgagatgc tcggggtgac cgcggccctc
       61 atcggccagg gcctcggcga gacggtggcc ctgatcacgg acgggcgctt ctccggcgcc
      121 acccgcggcc tgatggccgg gcacgtggcc ccggaagcgg cccaccgtgg acccatcgcg
      181 gcacttaagg acggcgacat cataaccttc gacatcgacg ggcggaccct gagcgtggac
      241 ctctcggacg aagagatcga agcccggctg gcggactggc aagaaccaga gccacgcttc
      301 gcgaccgggg ttatggccaa gtacgctgcc ctcgtctcct cggcctccga aggcgccgtc
      361 acagacccca acctcggaac cacagggtag cgtaaaggac caagacaccc agtataaccc
      421 cacgaaggag aacagcccct gggggcagcc ctccttcgtg gggtgggcag gctatccatt
      481 cgtatcgagg acctccgaga tagtttcgtc gagggccgac tcgtggcccc gattccatgt
      541 ggtctcgaag gcttggtctc cgagagctgc gcgggcgagc gccgcggcgt ggtcgtgatc
      601 cgatctcgtg agccgggtca ggcgcgcgcc cacctcctca cgcacggctt cggacgtccc
      661 aagcaatcgc gccgcccgct ccggccgccc gttgatggcc tccacccggg ccaagccatc
      721 caggcagaag aacacctcga aggtgaaacc gtattgccgc gagagagcca ggctttcttt
      781 gtgaagttcc cgggcccgta cgtacctgcc caactcacac tctatgcttc cgagcctggc
      841 cagcgtgatc gcgaccgcgt tggcgacgcg catcgggtag gtctcgtgcc gcagagaggc
      901 caggctctct tccagcagcg atctcgcccg ttctatctct cccttcgcgc gcagggcgtc
      961 cgcgagggta cagactgcca ggccccggcc aaaggcgcta tccgactcgc gggaggcggc
     1021 gagtgcctgc tcgctaagcg ataccgccag ttcgtaatca ccccgatgat acgccgcgac
     1081 gccgagctcc cgcaggggac cgctggtacc tctcgtgtgg cccatagttc tatagagcga
     1141 caggctctcc tgatagagtt cgaccgcacg gtcgtagtca ccctgccccc cggccaggat
     1201 acccgctacc aggtggagaa acgcccactg gcgtgcgggg agcgagggtt cggacaaccc
     1261 gctctccggc gggcccagcg ccaggatcgc ctccaaccac ccgcggcctt cggtcacgtg
     1321 gtgcctctcc ggccagaagc gccacatcat cagggcaagc ctcgtaccga aatccacatc
     1381 tccgcttcgt atcgcccagc gaagggccgc ccggaggttt tcgtgctccc tttccagcac
     1441 ctccagccac ctccccaaca taagttggga gacttcgggc tgcgcggcct cggcaagcgc
     1501 cagatagtag gcggcgtgcg cgcggtgcac cctctcggcc tcaccgtcag aagccaggcg
     1561 ctcagcagca tactcgcgaa ttgtctcgag catcgtgaaa cgcggctcct cgtcgcctgg
     1621 ccgcccgctg tctgcggagt tcgacaccac caggctgtta tccaccagcg aggccaggat
     1681 ctcgaaggca cttccctcat ccgtttggca cacagcctcc gccgcctcca gggtgaagcc
     1741 accggcaaag accgagaggc gtccaaagag cgcctgttcg gcgggtttca ggagatcgta
     1801 gctccaggcc accgtatcgc gcagcgtctg atgcctctct ggcacgtcgc gtgcaccccc
     1861 cgagagcagc gacagacggt ggtcaaggcg ctccagtagc gcgtgaggag cgaatatctt
     1921 taccctggcc gccgcgagtt cgatggccag cggtagcccg tcgagcctcc tgcagatctc
     1981 cgccacctcg gcatcgcccg aggggccagg ggcgaagaaa ggcgctactg ccctggcacg
     2041 ctcctcgaac agccgtaccg cctcggaacg ccccgaggat gcttctgcgt ctattgagag
     2101 cgggggaacg ggaaactgcc gctctacgct tagccggagc ggcgcccggc tcgtcgccag
     2161 caccgtgagt tcaggacact ccctgaggag gtctgccacc accggaacgg cctcgggaag
     2221 gtgctcgaag ttgtccagca cgagaagcct ctgctctcct gccaggccct gcaacagcga
     2281 ctggtacatc gaccggtttc ctacgtcctc tattcccagc tcgttggcga tgacggaagg
     2341 gacgaggtcg ggatcccgca cgggtgccag ccgtacgaag gtggccccgt cccggaacgt
     2401 ctctgagcac cgaccggcca cctcgagcgc cagccgtgtc ttccctaccc caccagtgcc
     2461 cgtgagggtg agcagctgca cctcgtcact gaggagcagc tcgacagcga tcgacacttc
     2521 ccgctccctt ccgatgaggg gcgtcgcggg taccggcagg gtacgaacgg acgttgcggc
     2581 cggcgcccga tggatggaag cgcgacgcgc ggccgcttcg aactccgtca gttcccgacc
     2641 ttccagttcc aacgcctcgg ccagcttctg cacggtgtag gagtgaggac ggcgtcgctt
     2701 gccacgctcg agcagcccga tcgcctgcgg cgtcagcccc gtgcggccgg caagttcttc
     2761 ctgcgtaacg ccagccgtgg cccggtggcg ccgcaacaag gcaccgaacg gtacccccgc
     2821 gccggttagt gcacccctca taatctgctc ctcggccctg tctatcgcgg tgccccgagt
     2881 ttacgatata cgacgctact cgccgagcac cccgggtatc agttgttgca ggtagagacg
     2941 ctggaccatc tgcctccggc tgttgacccc tgtcttctcg aagatgctcc tgaagtggtt
     3001 gttgaccgta tgctgcgaga tgaatagcgt ctccgagatc tctttcgtgg aagaaccgcg
     3061 tgcggccagc ctcactatct ccgcctcgcg gcccgtaagg ccgtaggagt cgaggttgag
     3121 cctggccact tcctccggcc cggaaggtga taccaccacc accgtctcgc tctctcgtcc
     3181 cggcgttgac tcggtcaacg aagcgtgcaa cgtcaaccag cgccccgaac gtcccctgat
     3241 ccgtaccctc ggcacgaggg caaggtcggc atccgagtcg ggggcgaggg cccgcttgag
     3301 cgctccggct gccatggtga cggcgatggg ggcgtccctt tcccatccgg ggccgagatc
     3361 ctcaacctcc gaaagcagtc gttctgcggc aggggtatag gaaagcactt caccctggcg
     3421 atccagcacg atcactcctg ggatacccgg tcccaaccgc tcttcgtcag cacgcgagcg
     3481 gagcgccgcg gttttcagcc cggccgccac gtgaggcgcc acgcgggcga
//
LOCUS       DBNGVD010000374         4044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1364858, whole genome shotgun sequence.
ACCESSION   DBNGVD010000374 DBNGVD010000000
VERSION     DBNGVD010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1364858"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..466)
                     /locus_tag="AC16CM_10090"
     CDS             complement(113..466)
                     /locus_tag="AC16CM_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140222.1"
                     /translation="MRAEGQRAEDWHPSREVILVWSILGVVLSLAGIISFAATYAFAR
                     GEDGGTVEISLLALVIPTLVVFVVLGVHEWIHGLTMACFGARPACAHSSEAATRTLAG
                     GAASILAVVSRPYVG"
     gene            complement(568..1209)
                     /locus_tag="AC16CM_10095"
     CDS             complement(568..1209)
                     /locus_tag="AC16CM_10095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3140223.1"
                     /translation="MAKVVVLDVDGTLMDTNYLHTEAWARAFEETGHRVPREKLHKEI
                     GKGAELLIRQFVDDDDVVEEIQNLHSEFYEGFQKFGHPLPGAKELLSSLKERGYEVWF
                     VTSAKDEELEHHMQELEAEDNIAGVVNSSSVDNPKPAPDIFEEALRRAGTTAEETVAL
                     GDARWDVEAAKSAGIRTVAVLSGGAFHTQDLEEAGAVAVYEDCAAVLESDFPE"
     gene            1242..2231
                     /gene="ygfZ"
                     /locus_tag="AC16CM_10100"
     CDS             1242..2231
                     /gene="ygfZ"
                     /locus_tag="AC16CM_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3140224.1"
                     /translation="MKYMRVAQNDANPNGLDGYAALTKGAAVVDLSDRKLLRLTGKDP
                     TAMLNAVLTNDVPTGEVRGAYALLLDPKGRVQTDLRAVKTGDGILLDMEQEGAEAAAE
                     ILGRYAPFSRVKMEKLSDWGVLGLYGPHANDLLGVSGLAEHEAKQVEIDGSPVLVVGV
                     AVPVAGYDLIAPSDTLVEIREFLMGRGAFAADPAAYDTARIGTGIPRFGTDVTPDNFP
                     GESAGVLERAVSFGKGCYPGQETVARMHYRGSPNKRLYRFELDPDQEEAPGAGDEILQ
                     NGSASAGVVGRLTSVAPFPVDGRVYALGYLARKADPGSPLRTRDARILAATQA"
     gene            2228..2521
                     /locus_tag="AC16CM_10105"
     CDS             2228..2521
                     /locus_tag="AC16CM_10105"
                     /EC_number="4.2.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate lyase"
                     /protein_id="HIR3140225.1"
                     /translation="MKRPEDCEGIGDVRQAIDTLDREIIQLIGRRAHYVDRAADFKTG
                     EQSVRDPERQRAMFGARRAWAEENGLDPDAIEALYRNLVSYFIDREMREWQKG"
     gene            2811..3560
                     /gene="sufC"
                     /locus_tag="AC16CM_10110"
     CDS             2811..3560
                     /gene="sufC"
                     /locus_tag="AC16CM_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR3140226.1"
                     /translation="MLKIENLRVEIDGQEIVKGLDLEVGEGEIHAIMGPNGSGKSTLA
                     NVLMGHPRYEVTGGSITFRGEDVFELEPDERAKLGMFLAFQYPSEVPGVSVANFLRTA
                     VNSVREEELSPMDMYRLIQEKMKIMQMDPKFAERYLNEGFSGGEKKRNEILQMLMLEP
                     KLAIMDETDSGLDIDALQVVSKGVNELRGPEFSAVIITHYQRILRYIEPDRVHVMLEG
                     RIVTSGGKDLANELEDKGYEWVRQEFGAQKS"
     gene            3576..>4044
                     /locus_tag="AC16CM_10115"
     CDS             3576..>4044
                     /locus_tag="AC16CM_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3140227.1"
                     /translation="MPEDRTIQELGLDEYKFGFHDPEEYFFKTPRKGIDPEIVAMISK
                     HKNEPEWMLEFRLKALESFFEKPTPTWGGDLSEMDFDEIYYYIRPMENQGRTWDDVPD
                     DIKNTFEKLGIPQAERDSLAGVGAQYESEVVYHSLKEEWENSGVVFMDMDGGLR"
BASE COUNT          809 a         1253 c         1267 g          715 t
ORIGIN      
        1 gtcccgcaag atctggggtc acggtcagat cctggacgct cctatcccgg tcgagatgtc
       61 cgtcgtgggg tctctatccc cgactccccg acctgggctt cacacatcaa acttacccca
      121 cgtatggccg ggacactacg gccaatatgg aggccgcacc accagcgagg gtcctggtag
      181 ccgcttcaga cgagtgggcg cacgccggtc gcgccccgaa gcatgccatc gtcaacccgt
      241 gaatccactc gtgtaccccc agcaccacga agaccacgag cgtggggatg acgagtgcca
      301 ggagggagat ctcgaccgtg ccaccgtctt ctccgcgggc gaaggcgtag gttgccgcga
      361 acgagattat cccggcgagc gaaagcacaa cgccaagtat gctccagacg agaatgacct
      421 ccctgctcgg gtgccaatcc tccgccctct gcccctcggc cctcatcctg tctccgctcc
      481 agcccgcggc gcgccctggc ggcagatacg aactgcgccg tacccggggt cgcgcggcgg
      541 acgcctcttc tgtgcttcct gggtttgcta ctcggggaaa tccgactcca ggaccgcggc
      601 gcagtcctcg tagacggcaa cggctccggc ctcttcgagg tcttgcgtgt ggaaggcacc
      661 gccggagagg acggccacag tacgtatgcc ggcgcttttg gcggcctcca cgtcccagcg
      721 ggcatccccc agcgcgacgg tctcctccgc cgtggtccct gccctccgca gggcctcctc
      781 gaagatatcg ggggcaggtt tggggttgtc cacggagctg gagttgacca cgcccgctat
      841 gttgtcctcg gcctccagtt cctgcatgtg gtgttctagc tcctcgtcct ttgcgctggt
      901 cacgaaccag acctcgtagc cgcgttcctt gagggaggag agcaactcct tcgccccggg
      961 gagcgggtgg ccgaacttct ggaaaccctc gtagaactcg gagtggaggt tctggatctc
     1021 ctcgaccacg tcatcgtcgt cgacgaactg gcggatcagc aactcggcgc ccttgcctat
     1081 ctccttgtgc agcttctccc gcggcacccg atgaccggtc tcttcgaacg cccgcgccca
     1141 cgcctccgtg tgcaggtagt tggtgtccat gagcgtgccg tcaacgtcga gaactactac
     1201 tttcgccatc aaaactcccc tcgtgcttta gaatcgaact tatgaagtac atgagagttg
     1261 cccagaacga tgcgaaccca aacggcctgg acggctacgc ggcgctgacg aaaggcgccg
     1321 ccgtggtcga cctctccgac cgcaagctgc tccgcctgac gggcaaggac ccaaccgcga
     1381 tgctgaacgc cgtcctgacc aacgacgtgc ccaccggaga agtccgcggc gcctacgccc
     1441 tgctcctcga cccgaaggga cgggtccaga ccgacctgcg cgcggtgaag acgggggacg
     1501 ggatcctcct cgacatggag caggaaggcg cggaagcggc ggcggagatt ctcggccgct
     1561 acgccccctt ctcccgggta aaaatggaga agctttccga ctggggggtt ctcggcctct
     1621 acggcccgca cgccaacgac ctgctcgggg tttccggcct ggccgaacac gaggcgaaac
     1681 aggtagagat cgacggtagc cccgtcctcg tcgtgggcgt cgccgtgccc gtcgccggct
     1741 acgacctcat cgcgccctcc gacacgctgg tcgagatccg tgaattcctg atggggcgcg
     1801 gcgcgttcgc cgctgacccc gccgcctacg acacggccag gataggaacg ggcatccccc
     1861 gcttcggcac agacgtaacg ccggacaact ttcccgggga gtcggccggt gtcctggagc
     1921 gggccgtgag cttcgggaaa ggctgctacc cgggtcagga gaccgtggcg aggatgcact
     1981 atcgcgggag cccaaacaag agactctacc gcttcgagct ggatccggac caggaagaag
     2041 cgcccggcgc gggagacgag atcctccaga acggaagcgc ctcggccggc gtcgtgggcc
     2101 gccttacatc cgtggccccg tttccggtgg acggcagggt ctacgctctt ggctatctcg
     2161 cccgaaaggc cgatccggga tcccccctca ggaccagaga cgcgcggatc ctcgcggcaa
     2221 cgcaggcgtg aagcgccccg aagattgcga gggcatcggg gatgttcgcc aggccatcga
     2281 cacgctcgac cgtgagatca tccagctgat aggtcgccgg gcacattacg tcgacagggc
     2341 cgccgacttc aaaaccggcg aacagagcgt ccgggaccca gagcgtcaac gggccatgtt
     2401 cggggcaaga cgcgcctggg ccgaagagaa cgggctcgac cccgatgcga tcgaagccct
     2461 ctaccgtaac ctcgtctcct acttcataga ccgcgagatg cgcgagtggc agaaaggctg
     2521 aggtatccgg tctctcctcg ggatcttgtc gggaatcgtt ctcgataaca gccgatttcg
     2581 gtccccgttc tttgttacaa gctgctttcg cgcagaagtc ctgaaagtgg ttcccgagaa
     2641 gccctgttgt ggcgtgatct ggatcacaaa aatccgtata aaaagccggg tttcgggtgt
     2701 ttcaggaaag gttcttgctc gtgatcgctt tacgttgtta ggggtagggt gctgtgattt
     2761 aatagcctta ctgtagcaaa gtttactcac aggcaaacgg caggggttcg atgctcaaga
     2821 tagaaaatct acgtgtagag atcgacggtc aggagatcgt gaagggtctg gacctcgagg
     2881 taggagaggg tgagatccat gcgatcatgg gcccgaacgg gtctggcaag agcactttgg
     2941 caaacgtgct catgggccac ccgcgctacg aggtgaccgg cggctccatc acgttcaggg
     3001 gcgaggacgt cttcgagctg gagccggacg agcgggccaa gctcggcatg ttcctggcgt
     3061 tccagtatcc gagcgaggtt cccggcgtct ccgtcgccaa cttcctcagg accgcggtca
     3121 actccgtgcg tgaggaagag ttgagcccca tggacatgta tcggctcatc caggaaaaga
     3181 tgaagatcat gcagatggac cccaagttcg ccgagaggta tctgaacgag ggtttctccg
     3241 gtggcgagaa gaagcggaac gagatcctgc agatgctcat gctcgaaccc aagctcgcca
     3301 tcatggacga gaccgactcg gggctcgaca tcgacgccct acaggtcgtg tccaaggggg
     3361 tcaacgagct gcgagggccc gagttctcgg cggtcatcat cacgcactac cagaggatcc
     3421 tgaggtacat cgagcccgac cgcgtgcacg taatgctcga gggtaggatc gtgaccagcg
     3481 gcggcaagga tctggcaaac gagctcgagg acaaaggcta cgagtgggtg cgccaggagt
     3541 tcggcgccca gaagagctag gggaagggaa gaaatatgcc agaagacaga accatccagg
     3601 agctcgggct cgacgaatac aaattcgggt ttcacgatcc ggaggaatac ttcttcaaga
     3661 ccccgaggaa gggcatagac ccggagatcg tcgccatgat ctccaaacac aagaacgagc
     3721 ctgagtggat gctcgagttc cgtctcaagg ctctggagtc gttcttcgag aagccgactc
     3781 cgacctgggg cggcgacctc tcggagatgg actttgatga gatctactac tacatcaggc
     3841 cgatggagaa ccagggtcgt acctgggacg atgtgccaga cgacataaag aacaccttcg
     3901 agaagctggg catacctcag gccgagcgcg actcgctcgc cggcgtcgga gcgcagtacg
     3961 agtccgaggt cgtctaccat tcactgaaag aggagtggga gaatagcggc gtcgtcttca
     4021 tggacatgga cggtggcctg cgcg
//
LOCUS       DBNGVD010000375         2812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5007742, whole genome shotgun sequence.
ACCESSION   DBNGVD010000375 DBNGVD010000000
VERSION     DBNGVD010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5007742"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16CM_10120"
     CDS             complement(<1..236)
                     /locus_tag="AC16CM_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024694.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3140228.1"
                     /translation="MPRFVIQEHYARSHHFDLRLERDGVLVSWAVPKVMPTHPKKNRL
                     AMRVDDHDMSHLDLVDETPVEGVPGTVAKSIWDRG"
     gene            complement(244..624)
                     /locus_tag="AC16CM_10125"
     CDS             complement(244..624)
                     /locus_tag="AC16CM_10125"
                     /inference="COORDINATES: protein motif:HMM:NF021525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HIR3140229.1"
                     /translation="MDSSFDTETLNLLDETEEVYIETRRDADSPEQRTIIWVVVVGSE
                     VFVRSVHGQKGRWYQRISAHPEGALIVGDARVPVQATPATGVETVDVVSAAFRSKYEK
                     AWPGPTAGIVRPEALPTTLKLSPA"
     gene            complement(617..1180)
                     /locus_tag="AC16CM_10130"
     CDS             complement(617..1180)
                     /locus_tag="AC16CM_10130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3140230.1"
                     /translation="MSETPDARTTNDRITFLRTLRAVRAFRPDPVPQEVLDDILEVAR
                     WSGSASNIQPWEMIVVQNRDTLGALASVDGYAVHLARAPLGIVLVMAGDREAQEGYDE
                     GRLAERIMLAAHAYGVGSSIGWIVGRGRDAARALLGIPGHKMVRTAISLGYPDGQASA
                     SRPGRGRARKPLDEIVHQEGYGSEDHG"
     gene            complement(1233..>2812)
                     /locus_tag="AC16CM_10135"
     CDS             complement(1233..>2812)
                     /locus_tag="AC16CM_10135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140231.1"
                     /translation="VVGLLVGLALARGLFTFLQGYLAERASQGVAYDLRDALFERIER
                     LSFSYYDRVQTGQLVTRLTSDVEQIRSFAGSGVVQLANAVVMLVGTAALLFYLDWQLA
                     LMALAVVPIIGLLLARFVGKIRPLFREVQQTLGRLNTVLQEDLAGVRVIRAFAREDYE
                     TGRYTSVNDELLYKNLTTVRVFSNNFPFVFLFANLGTLGIIWFGGWQVIGGRLSIGDL
                     VAFNTLLGFMLFPILTIGFLSASISRAGASSQRVFDVLDAPLDVTDAPDASPLPHLAC
                     RVEFDDVAFRYPGTDREVFGEISFSARPGQTVAILGTTGSGKSTLVNLIPRFYDVTSG
                     AVRLDGHDVRDVTLSSLRSQIGIVLQETRLSSGTVRDNIAFGKPDATDEEVVAAAEAA
                     QADRFIRGLADGYETVVGERGIGLSGGQRQRIAIARALLIDPRLLILDDSTSAVDAET
                     EAAIQETLDRLMREQHRTVFVIAQRVSTVRDANLILVLDEGRIAASGTHEELLEDSEL
                     YNEILGSQLVSPTDTAA"
BASE COUNT          464 a          929 c          930 g          489 t
ORIGIN      
        1 ccacgatccc agatactttt cgccacggtc cccggaacgc cctcgaccgg cgtctcgtcc
       61 acgaggtcga ggtgggacat gtcgtggtcg tccacgcgca tggcgagccg gttctttttg
      121 gggtgggtgg gcattacctt cggcacggcc caggagacga gcacgccgtc cctttcgagc
      181 cgcaggtcga agtggtggct cctcgcgtag tgctcctgga tcacgaaccg gggcatagag
      241 gtctcaggcc ggggagagct tcagggtcgt tggcagcgct tcgggtcgaa ctatacccgc
      301 cgtcggaccc ggccacgctt tctcgtactt gctcctgaac gcggcgctca ccacgtcaac
      361 cgtctctacg ccggtggctg gagtggcttg cacgggaacg cgggcgtctc ccacgatcaa
      421 cgcgccttcg gggtgggccg agatcctctg gtaccaacgt cccttctggc cgtggacgga
      481 gcgcacgaag acctcgcttc ccacgaccac aacccagatg atcgtacgtt gttccgggga
      541 atccgcgtcg cgcctggtct ctatatagac ctcttcggtc tcgtcgagga ggttcagggt
      601 ttcggtatcg aaggagctat ccatggtcct ctgaaccgta cccttcctgg tgcacgatct
      661 cgtcgagcgg cttccgggcc cgaccacggc cggggcggga cgcgctggct tgcccgtcgg
      721 ggtagccgag ggagatggcg gtcctcacca ttttgtgccc gggtatgccg agcagcgctc
      781 tagccgcgtc cctaccccgg cccacgatcc aaccgatgct cgagcccacc ccgtacgcgt
      841 gggcggcgag catgatccgt tccgcgagcc gtccctcgtc gtagccctcc tgcgcctcac
      901 ggtcgccggc catgaccagg acgatcccga gcggggcccg cgccaggtgg accgcgtagc
      961 cgtcgaccga ggcgagggcg cccagcgtgt cccggttctg gacaacgatc atctcccacg
     1021 gttggatgtt ggatgcgctc cccgaccagc gtgccacctc cagaatatcg tcgaggacct
     1081 cctgcggtac cgggtctggc cggaaagcac ggacggcccg caaggtgcgc agaaaggtga
     1141 tgcgatcgtt cgtggtcctt gcgtcggggg tctcgctcat ggtcttctcc tgtccacgtc
     1201 ggtagccgtg cccttcgctt tcccccgcgc gctcacgcgg cggtgtccgt gggggagacg
     1261 agctgggagc cgaggatctc gttgtatagt tcgctgtctt ccagcagttc ctcgtgggtg
     1321 ccgctggccg cgatccggcc ctcgtcgagg accaggatca ggttcgcgtc ccgcacggtc
     1381 gagaccctct gggcgataac gaacacggtc ctgtgctgct cgcgcatgag cctgtccagg
     1441 gtctcctgta tggcggcctc cgtctcggcg tccacggcgg aggtgctgtc gtcgagtatg
     1501 agtagccgcg ggtcgatgag gagcgccctt gctatggcga tgcgctgccg ctgcccgccg
     1561 gagagcccga tgccccgctc accgaccaca gtctcgtagc cgtccgcaag cccccggatg
     1621 aacctgtctg cctgggccgc ctcggcggcc gccaccacct cctcgtcggt cgcgtcgggc
     1681 tttccgaagg cgatgttatc gcgcacggtg cccgaggaga ggcgcgtctc ctgcaggact
     1741 atcccgatct gggaccgaag ggaggagagc gtcacgtcgc gcacgtcgtg gccgtcgagc
     1801 ctgacggcac cgctggtcac gtcgtagaag cgtgggatca ggttgaccag cgtggatttg
     1861 cctgagccgg tggtcccgag gatggcgacg gtctggcccg gcctggccga gaacgagatc
     1921 tcgccgaaca cctcgcggtc cgtaccaggg taacggaagg ccacgtcgtc gaactcaacc
     1981 cggcaggcga ggtgcggaag tggggaggcg tctggggcgt ccgtaacgtc gagcggggcg
     2041 tcgagcacgt cgaagacccg ctgcgaggag gccccggcgc gcgagatgct cgccgagagg
     2101 aagcctatcg tcaggatcgg gaagagcatg aaaccgagca gcgtgttgaa cgcgacgagg
     2161 tccccgatgg agagccttcc acctatcacc tgccagcccc cgaaccagat gattccaagc
     2221 gtccccaggt ttgcgaacag gaacacgaag gggaagttgt ttgagaatac ccgcacggtg
     2281 gtgaggttct tgtacagaag ctcgtcgttg accgatgtat agcgtcccgt ctcgtagtcc
     2341 tcgcgggcga aggcccgaat aacgcgcacc cccgccagat cctcctgcaa caccgtgttc
     2401 aggcgtccga gcgtctgctg gacctccctg aacaggggcc ttatcttccc gacgaaccgc
     2461 gccagcaaca gcccgatgat gggaaccacg gccagcgcca tgagggcgag ttgccagtcc
     2521 aggtagaaga gcagcgccgc cgtgccaacg agcatcacga cggcgttggc gagctgcacc
     2581 acgccggagc cggcgaagct gcggatctgc tccacgtcgc tggtaaggcg ggtgactagc
     2641 tggccggtct ggaccctgtc gtagtaggag aaagagaggc gctctatcct ctcgaagagc
     2701 gcgtcacgca ggtcgtaggc gacgccctgc gaggccctct cggccaggta accctgcagg
     2761 aacgtgaaca ggccccgcgc cagcgcgagc cccacgagca acccgaccac ga
//
LOCUS       DBNGVD010000376         3523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2820636, whole genome shotgun sequence.
ACCESSION   DBNGVD010000376 DBNGVD010000000
VERSION     DBNGVD010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2820636"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="AC16CM_10140"
     CDS             complement(<1..1138)
                     /locus_tag="AC16CM_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3140232.1"
                     /translation="MAQNLRELLDRRVLIGDGAVGTLLGERGVGFGHPYARANLSHPG
                     MVADIHEEYLRAGADIIETNTFAANRFKLETHDLEDRVRDVNARGASLALRAAENVAS
                     TGDRALVLGTVGPLGRPLAPVGPVGPEEARSVFLEQAEALLDGGADAILLETFTDLVE
                     LRLAYEAVEPLGVPVLAYKTFVEDGETLAEGLPGRVAREISSWGTVLTGSNCTVGPQR
                     MVEITDQMADVAGPVAALPNPSLPQLVDGQIRFRRHTRHFARYGVKLAEAGARLIGGC
                     CGTTPAHVKALSEALEDFEVRPTTVHRRVNVLEKEQRAEAAAEPVSEFARRLRTGFAV
                     TVEVDLPRGNEIDQVVEAARRLKERGVHAIDISDGARARLRMHPV"
     regulatory      complement(1200..1312)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1500..2384)
                     /locus_tag="AC16CM_10145"
     CDS             complement(1500..2384)
                     /locus_tag="AC16CM_10145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140233.1"
                     /translation="MVMVQYQGAPSRVPLTESGAIPVAKGTPLSAREDPGRLRTTFDD
                     VASLYDEVRPGYPERLFDDLTALSGTGEGAKALEIGCGTGQATLPLARRGYRILGVEL
                     GVNLAAVARTKLADHPNARVLTCSFEEWPLEERAFDLLVSATAFHWVDPRVRYRKSAR
                     VLRPDGSLALIWNRADPEGSSEGFIEALEEIHRREAPELAPERRPPRLDWDADKAGEI
                     ERSGFFERPEERVYRFGVAHGAESYLRLLSTYSAHRSLDEVTRRRLFMAVGQLIDEEY
                     GGHVIEGYRSELYVSRKR"
     gene            2736..>3523
                     /locus_tag="AC16CM_10150"
     CDS             2736..>3523
                     /locus_tag="AC16CM_10150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3140234.1"
                     /translation="MSWPDVLDEYFRHRAAQDLFSGAVLITQGATRLYARAYGYASRT
                     WHVPNTLDTRFDTASVTKLFTAIATLQLIDRGLLAFDTPVVERLGLQGTTLSKEVNVL
                     HLLIHTSGIGDDADEEAGERYEDLWKTKPNYSVVETRDFLAQFANKLANFPPGQGCRY
                     CNCGYVLLGLLIESISGMTYRDYIRAHVFAPAEMTASGFFRMDRVNHNVAEGCDPIRD
                     ATGTIVDWKKNIYSFPPIGSPDGGAHVTVADLDRFLRAVRAGKLL"
BASE COUNT          602 a         1185 c         1113 g          623 t
ORIGIN      
        1 ccacggggtg catcctgagc ctcgcccgcg ccccgtcgga gatgtctatg gcgtgaacgc
       61 cacgctcctt caaacgccgc gctgcctcta cgacctgatc tatctcgttg ccacgcggga
      121 gatccacctc caccgtgacc gcaaagcccg tgcgtagcct ccgggcgaac tccgagacag
      181 gctcggcggc tgcctctgcc cgttgctcct tctccaacac gtttacgcga cggtggaccg
      241 tggtgggacg aacctcgaaa tcttctagcg cctccgagag cgccttgacg tgggccgggg
      301 tagtaccaca gcagcccccg atgagcctgg cgccggcctc ggcgagcttc acaccgtacc
      361 gggcgaagtg cctggtgtgc cggcggaagc gtatctgtcc gtccacgagc tgcgggaggc
      421 tggggttggg aagtgcggcg acgggaccgg ccacgtctgc catctggtcg gtgatctcca
      481 ccatcctctg cgggcccacg gtgcagttcg agccggtgag gacggttccc caggaggaga
      541 tctcccgcgc cacccgccct ggcaggccct cggcgagcgt ctcgccgtct tcgacgaagg
      601 tcttgtacgc gagcaccggt acgcccagag gctcgaccgc ctcgtaggcg agacgcagct
      661 cgacgaggtc ggtaaaggtc tccagcagga tcgcgtcggc cccgccatcg aggagcgcct
      721 cggcctgctc gaggaagacg gagcgggcct cttccgggcc gacggggccg acgggggcga
      781 ggggacgccc cagcgggcct acggtcccga gcacgagcgc ccggtctccg gtcgaggcga
      841 cgttctctgc cgcgcgtaag gcaaggcttg cgccccgcgc gttcacgtcg cgtaccctgt
      901 cttccaggtc gtgggtctcg agcttgaagc ggttggcggc gaaggtgttg gtctctatga
      961 tatccgcgcc ggcccgcagg tattcctcat gaatgtccgc gaccatcccg ggatgggaga
     1021 ggttggccct ggcgtagggg tggccgaagc cgacgcctcg ttcgcccagc agcgtaccga
     1081 cggccccgtc cccgatcagg acgcggcggt cgagtagctc tctgaggttc tgggccaaag
     1141 gaaacctcct cgctgtagtc acacatcgcg cggaggagag aggttgagtc tgccgattaa
     1201 cctcatcttc cggagtctgc tccgcgggag ttggcacctg ccacgagaga tcgctctccc
     1261 accggttgcc gcggcttcga agggcccgtc cctccaccgc tctcgatgag cgtcgcggtt
     1321 ttccgcccgc gacgggccca ttacagcacg gcgagaccgg catttcaatg ccgccgggga
     1381 cggctctccg cggccgagtt caagagcgcc accaggaacc acgcgcgaag cacgccgcgc
     1441 atgtcgctac tatgacgttc tgaaaagttc ggcacactac tgtcgtctcc cggaacgact
     1501 caacgcttgc gggacacgta gagctcgcta cggtaaccct cgatgacgtg gcctccatac
     1561 tcctcgtcga tcagctgccc cacggccatg aagagtcggc gcctggtcac ctcgtccagg
     1621 ctcctgtgag ccgagtaggt gctcagcaac cgcagatagc tctcggcgcc atgggccacc
     1681 ccgaaacggt agacccgctc ctcgggcctc tcgaagaacc ccgagcgttc tatctcgccc
     1741 gccttgtcag catcccagtc caggcgaggc ggcctccgct ccggggcgag ctctggcgcc
     1801 tcgcgacggt ggatttcctc gagggcttcg atgaaaccct cgctgctgcc ttcggggtct
     1861 gcacgattcc agatgagcgc gagcgaacca tccggccgca gtactcgagc gctcttgcgg
     1921 tagcggaccc ggggatcgac ccagtggaac gccgtagccg agacgaggag gtcgaaggcc
     1981 ctctcctcaa gcggccactc ctcgaaggag caggtgagca cccgcgcgtt cgggtggtcg
     2041 gccagcttcg tcctggcgac ggccgcaagg ttcacgccga gctctacgcc caggatgcgg
     2101 tagccccgcc gcgcgagggg tagcgtcgcc tggcccgtcc cacagccgat ctcgagcgcc
     2161 ttcgctcctt cccctgtgcc cgaaagggcg gtcagatcgt cgaagagccg ttcggggtag
     2221 ccgggacgga cctcgtcgta gagggaagcc acatcgtcga aggtggttcg cagccgcccc
     2281 gggtcttctc ttgcgctcag cggggttcct ttcgcgacgg gtatggctcc gctttcggtc
     2341 aggggaactc gggaaggtgc tccctggtat tgaaccataa ccatgagatc ttctccccta
     2401 cgagaccttc gagctcgagt ttgtggggca gcggtgccaa gcggcgcatt ttcgtgcgca
     2461 tcacgaaggc gcgtgatctc ccgtagggga cgaggggtca tgctggcccc gcggcggttc
     2521 tcgcgctccc gagaggagcc agttcttcaa cacgctgcgg tcttctctgg agtacgcctc
     2581 gccaagcccg gggcctacgc aagatacgct agcaattgct acgatcgtcc gaaggtgcgg
     2641 ccatgttgcc tcgaaggggc accgcagccc ccagctctcc tccacagcga tgtcgaaggt
     2701 taccggggca cgatggagcg cttgggaagg acgtcatgag ttggcccgac gtactggacg
     2761 agtattttcg gcaccgagcc gcccaggatc tgttctccgg cgcggtgctc atcacgcaag
     2821 gcgcaacccg gttgtacgcc cgcgcctacg gttacgccag ccgcacgtgg cacgtgccaa
     2881 acaccctgga tacgcgcttc gatacggcgt cggttaccaa actctttacc gcaatcgcga
     2941 cgttgcagct gatcgacaga gggctgctgg ccttcgatac tcccgtggtg gagcgcttgg
     3001 gcctgcaagg cacgacgctc tcgaaggaag tcaacgttct ccacttgctg atccatacct
     3061 cgggcatagg cgacgacgcc gacgaagagg cgggcgaacg gtacgaagac ctgtggaaga
     3121 cgaagcccaa ctactccgtc gtcgaaaccc gcgacttctt ggctcaattc gccaacaaac
     3181 tggccaactt ccccccagga caagggtgcc gctattgcaa ctgcggctac gtccttttgg
     3241 gcctcctgat cgaatcgatc agcgggatga cgtaccgcga ttacatccgc gctcacgtat
     3301 tcgccccagc ggaaatgacc gcctccggtt tcttccggat ggaccgcgtc aaccacaatg
     3361 tcgccgaagg atgcgatccg atccgcgacg caaccgggac catcgtggac tggaaaaaga
     3421 acatctactc cttcccaccg atcgggtcgc cggatggtgg cgcccatgtc acggtcgccg
     3481 acctggaccg attcttgcgg gcagtgaggg caggcaagct cct
//
LOCUS       DBNGVD010000377         6005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4097270, whole genome shotgun sequence.
ACCESSION   DBNGVD010000377 DBNGVD010000000
VERSION     DBNGVD010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4097270"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="AC16CM_10155"
     CDS             <1..855
                     /locus_tag="AC16CM_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3140235.1"
                     /translation="RLVVMARQFDVLGAVYAKAIGFNVSGSYGTSPNFVYSGVKKDGE
                     PFQTMEILYGGIPAIPGKDGLDGHSWWPEFLAVPAEYMETYYPMLVEEYSVRRDSCGA
                     GTFRGGCGIRKVYRFLAGGRITYQDDRHHTYPYGVDGGKPGAASKKTLIRAGGERIEM
                     PSKVRDVPVFEGDRLVFETAGAGGVGDPLERDPEAVAKDVRWNLQSPETAENVYGVVV
                     SEDGSVDESATESRREEIGRNRDELPGFDHGELPPLEEQRRVIAETRRAFDAWLSGEL
                     GKERARDG"
     gene            845..1513
                     /locus_tag="AC16CM_10160"
     CDS             845..1513
                     /locus_tag="AC16CM_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3140236.1"
                     /translation="MTDNVPTRFEGFGGRAGFGRRPALVVIDLNLGFTDPESPLGCDL
                     DGPISEVQKLLEAARAAEIPIAFTTIAYRESDRHTAAAFIDKVPALLTLEAGSRWAEI
                     DPRVSPRPSEPVLNKLFASGFFGTGLSSFLTAAGVDTLIVTGATTSGCVRATVVDALQ
                     YGFRPLVPREAVGDRNPDAHEANLYDIDAKYGDVIPAQEALDYLRAGGVAERSEGKDR
                     APET"
     gene            1577..2686
                     /locus_tag="AC16CM_10165"
     CDS             1577..2686
                     /locus_tag="AC16CM_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018398386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIR3140237.1"
                     /translation="MDVKAAVAFEADQPLKIETVQLEGPKEGEVLVEIRATGVCHTDE
                     FTLSGADPEGLFPSVLGHEGAGVVVEVGPGVTSVAEGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRSTQGQGLMPDGTSRLSLNGEPLYHYMGTSTFATHAVLPEIAVARIREDAP
                     FDKVCYIGCGVTTGIGAVIYTAGVTPGSNVVVFGLGGIGLNVVQGARMVGADKIIGVD
                     LNPAKRDLAEKFGMTHFVNPDEVEGDLVPYLVDLTDGGADFSFECIGNVNVMRQALEC
                     AHRGWGISTIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGK
                     INIDDLITHTMPLEEINTAFDLMHRGESIRSVVQF"
     gene            2804..3658
                     /gene="fghA"
                     /locus_tag="AC16CM_10170"
     CDS             2804..3658
                     /gene="fghA"
                     /locus_tag="AC16CM_10170"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HIR3140238.1"
                     /translation="MTDLKTLGEHACFGGTVSFHEHHSPACDAGMRFAIYLPPQASER
                     PVPVLYYLAGLTSTEETFMIKGGAQRLAAEHGLMLVAPDTSPRGLGLPGEDDDWDFGT
                     GAGFYLDATAEPFSHHYKMDTYVTRELPELVAEHFPASDARGVFGHSMGGHGALTLAL
                     KNPDLYASVSAFAPVSAPMRAPWGQKAFPGYLGDDEESWREHDASELVLRRPFADGRT
                     ILVDQGTADEFLEEQLYPEVLESACREAGQPLTLRWHEGYDHNYYFISTFMADHVRHH
                     AEALAGSS"
     gene            3706..4962
                     /locus_tag="AC16CM_10175"
     CDS             3706..4962
                     /locus_tag="AC16CM_10175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3140239.1"
                     /translation="MEETGSTLTHLEGALSGTNYPADDLMGLDPSDGRPLLARYDLER
                     AARTLTTESLASRRTGGLWRWKELLPVKDAGKVVYLGEGDTPLLSPSRLGEALGIPGL
                     TIKAEGLNPTGSFKARGMAVAVSRAVELGVTAFIAPSAGNAAGALAAYGAAAGTQVTV
                     LTPVDATPANRQEVLVMGGRLILVQGLISDCGKISTALADRIGAFDVSTLKEPYRVEG
                     KKTMGFELAEQCGWRLPDVIVYPTGGGTGLVGMWKAFDELEGLGLIGPHRPRMVSVQS
                     EGCAPIVRAFEGGERFAEPWQNAMTRASGIRVPSAVGDFLILDALHESGGTAVAVPEG
                     AISEMQMLAGRYGGGYVSPESAAALAAVPLLRERGEVDRNENVVVFDCGIGQKYPPPH
                     DLPEPPTVDPQHLDIEALAGLLNPET"
     gene            complement(4966..5382)
                     /locus_tag="AC16CM_10180"
     CDS             complement(4966..5382)
                     /locus_tag="AC16CM_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3140240.1"
                     /translation="MKVDAANYGLQREYDQPVDIHLPGTVGFESERTLSTVAHLTTFL
                     NIFTGFLGPVAAFVIWLVYRDDSPTVAAHAMRSVMYQVVWLTAIFVGWSVTFALMAIL
                     VGFLLVPIMLLVTLGPFVQASYEAYVAYRDTGRRYY"
     gene            5581..>6005
                     /locus_tag="AC16CM_10185"
     CDS             5581..>6005
                     /locus_tag="AC16CM_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3140241.1"
                     /translation="MTEESQNTGNGRGNTATGVESLSVTDNRTGANYEVEIKDGTVRA
                     MDFRQIKVDDDDFGLMTYDPGYSNTASCRSAITYIDGEKGILEHRGIPIEQLCEQSSY
                     LEVAYLLNYGHLPAEDELNRWTYDITHHTYVHENIKNFM"
BASE COUNT         1151 a         1938 c         1927 g          989 t
ORIGIN      
        1 cgcctcgtcg tgatggcgcg acagttcgac gtgctcggcg ccgtctacgc caaggccatt
       61 ggcttcaacg tctcaggctc ctacgggact agccccaact ttgtctactc cggcgtcaag
      121 aaggacggag agccgttcca gacaatggag atcctctacg gtggcatccc ggccatcccc
      181 ggcaaggacg gcctggatgg gcactcctgg tggcccgagt tcctggcggt gcctgcggag
      241 tacatggaga cctactaccc gatgctagtg gaggagtact cggtccgtcg cgactcctgt
      301 ggcgccggca cgttcagggg cggctgcggt atcaggaagg tctaccgctt cctggccggt
      361 ggccgcatca cctaccagga cgatcgccac cacacctacc cttacggggt ggacggcggt
      421 aagcccggcg cggcgtcgaa gaagaccctc atacgggccg gtggtgaacg tatcgagatg
      481 ccttccaagg tgcgcgatgt gcctgtcttc gagggagacc gcctggtctt cgagacggcc
      541 ggcgccggcg gggtcgggga tcccctggag cgcgaccccg aggccgtggc caaagacgtg
      601 cgctggaacc tgcagagccc cgagaccgcc gaaaacgtct acggcgtggt cgtctccgaa
      661 gacggttccg tggacgaaag cgccactgaa tccaggcgcg aggagatagg gagaaaccgt
      721 gacgagctgc ccggcttcga tcacggagag ctgcccccac tcgaagagca gcggcgcgtg
      781 atcgccgaga cccgccgcgc gttcgacgcc tggctctccg gcgagttggg aaaggagcgc
      841 gcccgtgacg gataacgttc cgaccaggtt cgaaggtttc ggcggacgtg ccgggttcgg
      901 ccgccgtccc gccctcgtcg tcatagacct gaacctaggg ttcacagacc cggaatctcc
      961 gcttggctgc gatctcgacg gccctatctc cgaggtacag aagctcctgg aagcggccag
     1021 ggctgcggag atccccatcg cgttcaccac cattgcctac cgggagtcgg acaggcacac
     1081 ggccgccgcc tttatcgaca aagttccggc gctcctcacc ctggaggccg gatcgcgctg
     1141 ggcggagatt gacccccgcg tgtctccccg cccgtcggag cccgtcctca acaagctctt
     1201 cgcctccggt ttcttcggca cggggctctc cagcttcctg accgccgccg gggtggatac
     1261 cctcatcgtt accggggcga ccacctcggg ctgcgttcgg gccaccgtcg tagacgcact
     1321 ccagtacggc ttcaggcccc tcgtgccccg cgaggccgtg ggggaccgca accccgacgc
     1381 ccacgaggcg aacctctacg acatagacgc caaatatggc gacgtgattc ccgcccagga
     1441 ggcgctggat tacctgcggg cggggggagt tgcagaacgt tccgaaggga aagacagggc
     1501 cccggaaact tgacgctggc gggtgttggc ccgaccatac gggccgtaat ggggctaccc
     1561 aggggacgga ggtcaggtgg acgtaaaggc ggcagtagct ttcgaggcgg accaaccgct
     1621 gaagatcgag acggtccagt tggagggacc aaaagagggt gaggtgctgg tagagatccg
     1681 ggcgaccggc gtctgccaca cggacgagtt cacgctctcc ggagcggacc ccgaggggct
     1741 cttcccctcc gtgcttggcc acgagggggc cggggtggtg gtcgaggtcg ggccgggggt
     1801 cacgagcgtg gccgaagggg accacgtgat cccgctctac accccggagt gccgccagtg
     1861 caagtcatgc ctgagccgca agaccaacct gtgcacggct attcgatcca cgcagggcca
     1921 gggcctgatg ccggacggca cgtcgcgcct ctccctgaac ggggaacccc tctaccacta
     1981 tatgggcacc tcgacgttcg ccacccacgc cgtcctgccg gagatagcgg tcgcccggat
     2041 ccgcgaggac gcaccattcg acaaggtctg ctacatcggc tgcggcgtaa cgaccggcat
     2101 aggagccgtg atctacaccg ccggcgtcac gccaggttcg aacgtggtcg tcttcgggct
     2161 cggcggcatc ggtttgaacg tggtccaggg cgccaggatg gtcggggccg acaagatcat
     2221 aggcgtcgac ctcaacccgg ccaagaggga cctcgccgag aagttcggca tgacccactt
     2281 cgtgaacccg gatgaggtgg agggcgatct cgttccctac ctggtcgacc tgaccgacgg
     2341 cggggccgac ttctccttcg agtgcatcgg taacgtgaac gtgatgcgcc aggcgctgga
     2401 atgcgcccac aggggctggg gcatctcgac gatcatcggg gtggccggct cggggcagga
     2461 gatctccact cgtccgttcc agctcgttac cggtagggtc tggaagggca gcgcgttcgg
     2521 cggcgccagg ggccgtaccg acgtgcccaa gatcgtggac tggtacatgg agggcaagat
     2581 caacatcgac gacctgatca cccacaccat gccgctcgag gagatcaaca cggccttcga
     2641 cctgatgcac cgcggcgaat ctatccgcag cgtggtgcaa ttctagaggg cttcaggcta
     2701 cagacatcag gaagaattac ctgatgcctg tagcctgata cttgaaacct tccttcaccc
     2761 caggttctat cctgccaaaa ccattctcaa aggaaaagag gctatgacgg atctaaagac
     2821 attgggcgag catgcctgtt ttgggggcac cgtctcgttt cacgagcatc actccccagc
     2881 ctgtgacgcc gggatgcgat ttgccatcta cctcccacct caggcctcgg agcggccggt
     2941 tcccgttctt tactacctgg ctgggctgac ctccacggag gagacgttca tgataaaggg
     3001 cggcgcccag cgtctcgccg cggagcacgg cctgatgctc gtggcccccg acacgagccc
     3061 gcgcggcctg gggctgccgg gagaggacga cgactgggac ttcggcaccg gtgccggctt
     3121 ctacctcgac gccacggccg aaccattctc gcaccactac aagatggaca cctatgtaac
     3181 tcgggagtta cccgagctcg tggcggagca ctttccagcc tcggatgcca ggggcgtctt
     3241 cggacactcc atgggggggc acggcgccct gaccctggcg ctcaagaacc cggatctcta
     3301 cgcctcggtc tcggccttcg ccccggtctc ggcgcccatg cgcgcgccct ggggacagaa
     3361 ggcgttcccg ggctacctgg gcgacgatga agaatcgtgg cgcgagcacg acgccagcga
     3421 gctggtgctc cggcggccct tcgcggatgg acggacgata ctcgtggatc agggaactgc
     3481 ggacgagttc ctcgaggagc aactctaccc cgaggtcctc gaatcggcct gccgggaggc
     3541 cgggcagccc ctgacgctgc gttggcatga aggttacgac cacaactact acttcatctc
     3601 gaccttcatg gccgaccacg taaggcacca cgcggaggcc ctggccggct catcctagac
     3661 cgtcgttaga ataccggtaa tctattgcgg ctagagagga gccatgtgga agagacgggt
     3721 tcgacgctaa cgcacctgga aggcgcactt tcgggcacga actatccggc agacgacctg
     3781 atgggcctgg atccgtccga cggaagaccc cttctcgccc ggtacgacct ggagcgggcg
     3841 gcccgtaccc tgaccaccga atccctggca agtcgccgca cgggcggcct gtggcgctgg
     3901 aaggaactct tgcccgtaaa ggacgcgggc aaagtcgtct acctgggcga aggcgataca
     3961 ccgctcctgt caccgtctcg gctcggtgag gcgcttggca ttccaggcct gaccatcaag
     4021 gccgaggggc tgaacccgac gggctcgttc aaggcgcgcg ggatggccgt cgcggtctcc
     4081 cgggccgtgg aactcggcgt caccgctttc atagctccct ctgccggcaa cgccgccggt
     4141 gcgctcgccg cctacggtgc cgccgccggg acgcaggtca ccgttctgac acctgtagac
     4201 gccacaccgg ccaacaggca ggaggtcctc gttatggggg ggcgcctgat cctggtccag
     4261 ggtctcatct ccgattgtgg caagatctca accgctttgg ccgataggat cggtgccttc
     4321 gacgtgagca cgctcaaaga gccttaccgc gtcgagggca agaagaccat gggtttcgag
     4381 ctcgcggaac aatgtggctg gcgcctaccc gacgtgatcg tctaccccac gggcggcggc
     4441 accgggctcg tcggcatgtg gaaggccttc gacgaactcg aagggcttgg cctgatcgga
     4501 ccccaccgac ccaggatggt gagcgtgcaa tcggaaggtt gcgcccccat cgtgagggcc
     4561 ttcgaagggg gagagcgctt cgccgaaccc tggcagaacg ccatgacccg tgcctctggc
     4621 atcagggtcc cctccgccgt gggagacttc ctgatcctgg acgcccttca tgagtcgggc
     4681 ggcacagccg tcgctgtccc cgagggtgcc atctccgaga tgcagatgct ggccgggcgt
     4741 tacgggggcg gctacgtgag cccggagtcg gcggcggccc ttgccgccgt gccgctgctc
     4801 cgtgagaggg gagaggtgga ccgcaacgaa aacgtggtcg tcttcgactg cggcatcggc
     4861 cagaagtacc cgccgccaca tgatctccca gaacctccaa ccgtcgaccc gcaacacctc
     4921 gacatcgagg ccctggccgg gctcctgaac cccgaaacct gaccgctagt aataccggcg
     4981 ccccgtgtcc ctgtaggcca cgtacgcctc gtaggacgcc tgcacgaagg gaccgagcgt
     5041 cacgaggagc atgatgggga ccagcagaaa accaaccaga atggccatga gcgcgaaggt
     5101 gacggaccag ccaacgaaga tggcggtgag ccagaccacc tggtacatga ccgaccgcat
     5161 cgcatgggcc gccaccgtcg gcgagtcgtc cctgtagaca agccagatca cgaacgccgc
     5221 gacaggcccg agaaaccccg tgaaaatgtt caggaacgtg gtcagatggg ccacggtcga
     5281 cagcgtacgc tcgctctcga agccaaccgt gccggggagg tggatgtcaa cgggctggtc
     5341 gtactcgcgc tgaagaccgt agttggcagc atcaaccttc atgagcctcc tctcgtggct
     5401 aaccgcgtat acgacttcac cgccagaagg tttcttgccg actggccccg tctttgcgct
     5461 cactttccga tcacgcgccg gtgggcgata atgcaaacat cttacggggc gcagggactc
     5521 agtcacatat gtggcacgag catatataat tagccgacgt gtaatagaaa ggggcacagg
     5581 ttgactgaag agtcgcagaa tacgggaaac ggccgaggta acacggcgac gggggtagag
     5641 agcctatcgg tcaccgacaa ccgcacgggc gccaactatg aggtcgagat caaggacggc
     5701 accgtccggg cgatggactt caggcagatc aaggtcgatg atgatgactt cggccttatg
     5761 acctacgacc ccggctactc caacacggcg agttgccgga gcgctatcac ctacatcgac
     5821 ggggagaagg gcatcctgga gcacaggggc atccccatcg agcagttgtg cgagcagtcc
     5881 agctacctgg aggtggccta cctcctgaac tacggccatc ttcccgccga agacgagctg
     5941 aaccgctgga cctacgacat aacccaccac acctacgtgc acgagaacat caagaacttc
     6001 atgaa
//
LOCUS       DBNGVD010000378        10031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_634410, whole genome shotgun sequence.
ACCESSION   DBNGVD010000378 DBNGVD010000000
VERSION     DBNGVD010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_634410"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="AC16CM_10190"
     CDS             <1..148
                     /locus_tag="AC16CM_10190"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3140242.1"
                     /translation="VHDTPGLYVFSGAAFPTCPGVNPTLTMWALCYRAAERLVGRLGR
                     GDEE"
     gene            273..1553
                     /gene="larA"
                     /locus_tag="AC16CM_10195"
     CDS             273..1553
                     /gene="larA"
                     /locus_tag="AC16CM_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR3140243.1"
                     /translation="MRTELQYGKGELEVEVPSSDVTVLRTKHEDGLPDEAAAFREAVR
                     NPIGSAPLGELVGAGDRVAVVIPDITRPLPSDRLLPWLFEELSHVPAENVTIVNGTGS
                     HRPNTEDELVAMVGREVASSYRIVNHDAHDPDTLMFAGRSSEGRDVYMNREYVEADRR
                     IVLGFIEPHFMAGFSGGYKGVFPAVADIDSIMHYHRASVIGHARSTWGVLEDNPTQDQ
                     IRANGAVLPVDFLVNVTINRDKEITGYFCGDVLSAHEKGCEANKASVMVACEREYPIV
                     VTTNGGYPLDQNLYQAVKGMSAAYQIVSDGGLIVAASRCNDGFPDHGNFRRMLLEHSS
                     PQAMLDTIYQPGFSEFDQWQVQLFAQILQRARVGLYSEIPADDVRSVHLEPISDIATT
                     IAEELERIGADAPIAVLPEGPMTVPYLEQAPVEA"
     gene            1992..3641
                     /locus_tag="AC16CM_10200"
     CDS             1992..3641
                     /locus_tag="AC16CM_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140244.1"
                     /translation="MMATVVQACDVGIIRGTRASAPCRPSSRPWVVAAASLGSGMAFL
                     DSTVLNVALPAVQAELEASAREAQWVYGAFALVLASFLLLGGSLGDRYGRRRVFVLGA
                     AIFAVASAWAALAPGPGQLIAARAVQGAGGALLVPASLAILGASFEGKSRAKAIGLWG
                     ALSATAMAVGPVLGGWLVEEVSWRIAFLIAPAMAVVAIPIALGHVPESRDPEAKGLDL
                     AGALLATTGLGGVVYALIESSALGLGHPAVLAALLLSAVALAAFVFVERRGKDPMVPP
                     SLFRSRDFDGANLVTLLFYMALTGSLYFVPFLMMQVHGYSAFVAGTVFLPFVAMALLL
                     GRLSGRICARFGTKVPLVVSSLAVAGGFLLFALPGAEPAPYWTAFFPAMVVQGFGMAL
                     VITPLTAVALGSVEGEHSGVASGVNNAAARVAGLLAVAVLGMFAYGMFSANLDARLDS
                     MNLPGEVRSEMEAAKADLGAAEAPEGVDAGTAARIERAVDESFVAGFRAVMLVSAGLA
                     FASALTAVLLVGNWRVRSVSRDSHPSGGLRARVRRHIHHIA"
     gene            4044..4739
                     /locus_tag="AC16CM_10205"
     CDS             4044..4739
                     /locus_tag="AC16CM_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HIR3140245.1"
                     /translation="MPDTLDDFAAREPSSAEAGRSRPTRLIVATNVVLAGLLSVLSFA
                     GVTEVTGISAYLGTFFLVGALVPSNLLRFERYASGRRTRALARLRKPLGISAGVWFVA
                     HSVVSVWELFDLSLPLLPQFTRTGIVLGLLATVVFVAMLATSTDRAQRALGRNWKRLH
                     RLVWFAVPLALAHTILASGLDSPSIVFYLGIMAFAGFEYHALRRRRMRGARIHIMLIG
                     AGVFAAALIYVVL"
     gene            5017..5166
                     /locus_tag="AC16CM_10210"
     CDS             5017..5166
                     /locus_tag="AC16CM_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140246.1"
                     /translation="MMQVTAEDVVELHLGLQTRGVQLWTDGGWGIGAKTLWIAKLSQI
                     AEFLS"
     gene            complement(5223..5465)
                     /locus_tag="AC16CM_10215"
     CDS             complement(5223..5465)
                     /locus_tag="AC16CM_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3140247.1"
                     /translation="MRAKRVSGTALSAILRKRDERPFASVADLYRRTPVWRAALANLI
                     GAGFLYRLRPRRDRVALLGETSDLCHARRSIRPPEH"
     gene            complement(5563..6339)
                     /locus_tag="AC16CM_10220"
     CDS             complement(5563..6339)
                     /locus_tag="AC16CM_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR3140248.1"
                     /translation="MNPQKIARVTGVLFLVTIVASIPAQFIMYGPVLNDPNYIVSAGA
                     DTLVRWGAVLEVITALACIGTAVVLFPIVKRQNEAVALSYVAVRVLEAAIIFAGIVSL
                     ISVVALRQGFAGAAAADAAPLLTVGKSLVAVHDSTFLLGPGILAGLGNGILLGYLMYS
                     SNLVPRRMALLGLVGGPLVSASGIAMLLGLYAPVSLWSVIATIPEFFWELSLGIWLIV
                     KGFNTSAVVSLSTDPDDGILTGVEQPAAMVPSNGHVPSKV"
     gene            complement(6358..6900)
                     /locus_tag="AC16CM_10225"
     CDS             complement(6358..6900)
                     /locus_tag="AC16CM_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140249.1"
                     /translation="MGEAATSVGSRPSLTSLKERSALPMSEVSTFRLYLLRAMYVFTF
                     VGLAIEKLPALLHPATLSPGDSVILSVLGATALLAVVGIRYPIKMLPLLFFEFVWKAI
                     WILLFGLPLLLSGGLDPNVSFGGTETLIACLVGVVLVPLVMPWGYVIEHYFKVPGARW
                     GKKQVTVRPPHPSDKPKIVT"
     gene            complement(7015..7662)
                     /locus_tag="AC16CM_10230"
     CDS             complement(7015..7662)
                     /locus_tag="AC16CM_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140250.1"
                     /translation="MVAGILFVVIQPIHPPDVPASVTTAQWAIVHYLGVAMCLFGLLG
                     LAGLYARQVEAAGWLGLAGYLLFSLFYALTMAFQFAEAFISPRLATESPRFVEGFLGI
                     FNGHPGEIDLGALPAVYGLTGGAYLLGGLVFGIAALRAGILPRWAAGLLAVAAPVAPV
                     AVALLPHALERYAAVPMGIALACLGYALWSERREQASAEPAPGTGTPQLHQTAAA"
     gene            complement(7850..8575)
                     /locus_tag="AC16CM_10235"
     CDS             complement(7850..8575)
                     /locus_tag="AC16CM_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR3140251.1"
                     /translation="MIIFIVGIPPAAFLLGPVLDNPDYIVSSGADNQVLLGVFLDLVN
                     ALACIGTAGALFSVVKRQHEGFALGFVTTRLFEAAVIVIGVVSLLAVVTLRQAGGAGT
                     DGAALVAVGRALVAVRDGAHLLGPGVIPGLNALLLGYLMYRSRLVPRFIPAMGLIGAP
                     VFLISAAASILGFNEQVSVLSGIAVIPIALWELSLGLWLTFKGFNPSAPILAAPAAEL
                     GSADTSAAIVPSSTAVATKATVA"
     gene            complement(8705..9409)
                     /locus_tag="AC16CM_10240"
     CDS             complement(8705..9409)
                     /locus_tag="AC16CM_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140252.1"
                     /translation="MFTKLTDTSKAAIFTVLVLFLSVGAALSINMLDLGSNMLMWASV
                     WAITPVVATLIMLLVVTRDGYSREGWKSLGLHRLGLNVWWIAFGMIFLASLVASIVVW
                     ATPLASFVLPEGGIYIPAINLLAEVVIPVEALLNGSLGIALTSASILFLLGTLAFVTA
                     LLRTRELPTAPLVLYAVGAVPISLRAFVPEAVLDLGLVILAVGIAWLAIWLFDRSSRI
                     APTWKMTGTPSADPVN"
     gene            complement(9457..>10031)
                     /locus_tag="AC16CM_10245"
     CDS             complement(9457..>10031)
                     /locus_tag="AC16CM_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3140253.1"
                     /translation="LLGAQAPSAADLGGWTGLFSTFAVVLLIPGTGGTWEEPGWRGFA
                     LPSLQAGRSALVASLILGAVWALWHLPLVVATGQMGGWDIVIILAWTLVLTWVYNGTG
                     GSVLIVMLFHAMFNTVSGSFISPLAFSGADSVRQSWLLAAVWCAVAVAVVMWAGPKHL
                     SRKLHKQEEEGGAEEPGTATPSGAVKPVPA"
BASE COUNT         1867 a         3085 c         3278 g         1801 t
ORIGIN      
        1 cgtacacgac acgccggggc tgtacgtctt cagcggggcc gctttcccga cctgtcccgg
       61 ggtcaacccg accctgacca tgtgggcgct ctgctaccgg gccgcagagc gcctcgtggg
      121 gcgtctcggg cgtggtgacg aagagtgact ggttcggcca cgataaagga gtttttggtt
      181 tgctcggtcg gggtacaggt cccgcgggtc aggtgtgagg taacaggcta gttcgcgcac
      241 cttgtaaggg gcgcgagagg aggctacgaa cggtgcgcac cgaattgcag tacggtaagg
      301 gcgagctgga ggttgaggtt ccgtcctcgg atgtgaccgt gctcaggaca aagcacgagg
      361 atgggttgcc ggacgaggcg gctgcttttc gcgaggcggt gcgcaatcct atcggctccg
      421 cgccgctcgg cgaactggtc ggagccgggg accgtgtggc agtcgtgatc ccggacatca
      481 cccgcccctt gccctccgac cgactgttgc cctggctctt cgaggagctc tcgcacgtgc
      541 cagcagagaa cgtaaccatc gtcaacggaa cgggctcgca ccggccaaac acggaagacg
      601 agctcgtcgc gatggtcgga agagaggtgg cctccagcta ccgcatcgtg aaccacgacg
      661 cccacgaccc cgacacgctg atgttcgccg gcagatcgtc cgaaggccgc gacgtgtaca
      721 tgaaccgcga gtacgtcgag gcggacaggc gcatcgtgct cggctttatc gaaccccact
      781 tcatggccgg cttctcaggc gggtacaaag gcgtcttccc cgccgtggcg gatatagact
      841 cgatcatgca ctaccaccgg gcctcggtga taggtcacgc ccgaagcacg tggggggttc
      901 tcgaagacaa cccgacgcag gaccagatcc gggcaaacgg cgccgtatta ccggtagact
      961 tcctcgtgaa cgtcacgata aaccgcgaca aggagatcac gggctacttc tgcggcgatg
     1021 ttctgtccgc ccatgagaag ggctgcgagg caaacaaggc gtcggtgatg gtagcgtgcg
     1081 agcgcgagta ccccatcgtc gtcacaacca acggcggcta ccccctggac cagaacctct
     1141 accaggcggt gaagggcatg tcggcggcct accagatagt ctcagacggg gggctcatcg
     1201 tagccgcctc tcgctgcaat gatggcttcc ctgaccacgg caactttcga aggatgctcc
     1261 tggagcactc ctcaccgcaa gcgatgctcg acacgatcta ccagccaggg ttctccgagt
     1321 tcgaccagtg gcaggttcag ctgttcgccc agattctgca gagagcgagg gtcggcctct
     1381 acagcgagat acccgccgac gacgtccgaa gcgtgcacct cgagccgatc tcggacattg
     1441 caaccacgat cgccgaggag ctcgaaagga tcggcgcaga cgcacccata gcggttctac
     1501 ccgaaggacc catgaccgtg ccctaccttg agcaagctcc ggtcgaggcc tgacggtcca
     1561 acacgcaagg tcacggataa cgctggggta ccgcgagagg gttgcttcgg tggtgggcga
     1621 tgctgggttc gaaccagcga cctctgccgt gtgaaggcag tgtgatagtt tgctggaggt
     1681 ttctggagct tgcaaaacac ctgcaaatta gcgtatttcc actttgatcc ttttcccaac
     1741 ctttcaggag atttacccgg gttgctgcac ggttgctgca caaagctagg cgtttcgaac
     1801 acttcatcta tcgggtcgcg acgagctaat gcttggcgtt cacgaccgtg cagggcgtct
     1861 ttacgactaa agtcttagta gcaattagat cctgtgcccg atgtactccc aactcgcctg
     1921 cgccaccatc aaggtgtaag gaaagcggac caacagggta tctccctaga ggcccttaac
     1981 taaggagcaa gatgatggcc acagtagtcc aggcgtgcga tgttggcatc atccgcggca
     2041 cccgcgcctc ggcgccttgc cggccttcct cgcggccgtg ggtggtagcg gctgcctccc
     2101 tgggttcggg gatggccttc ctcgacagca cggtgttgaa cgtggccctg ccggcggtgc
     2161 aggctgagct cgaagcgagc gcgagagagg cgcagtgggt atacggggcc ttcgccctcg
     2221 tgctggcctc gttcttgctc ctcgggggct ctctgggcga tcgctacggg aggcggcgcg
     2281 tcttcgttct cggtgcggcg atcttcgctg tcgcctcggc gtgggctgcc ctcgcccccg
     2341 gccccgggca actcatcgcg gcgcgggccg tgcagggtgc gggcggggcg ctcctcgtgc
     2401 cggcgtcgct ggcgatcctc ggggcatctt tcgaggggaa gtccagggcg aaagcaatcg
     2461 ggctttgggg ggcgctaagc gccacggcga tggcggtagg gccggtcttg ggcggctggc
     2521 tggtcgagga agtctcctgg cggatcgctt tcctgatcgc cccggcgatg gccgtcgtgg
     2581 cgataccaat cgcgctgggg cacgtgccag agagtcgcga cccagaggcc aaagggctgg
     2641 atctcgctgg cgcgctcctt gccacgaccg ggctcggggg ggtggtctac gctctgatcg
     2701 agtcttcggc tttggggttg ggccacccgg cggtgctcgc cgctctcctc ctgagcgccg
     2761 tcgcccttgc ggccttcgtc ttcgtcgaac ggcgtggcaa ggatccgatg gtgccgccat
     2821 cgctgttccg ttccagggac ttcgacggtg cgaacctggt gacgctcctg ttctacatgg
     2881 cccttaccgg ctcgctgtat tttgtgcctt tcctgatgat gcaggtccac ggctactcgg
     2941 ccttcgtcgc gggcaccgtg ttcctgccgt tcgtggcgat ggccctccta ctcggtcgtc
     3001 tgtccggtcg tatctgcgcc cgcttcggga cgaaggtgcc gctcgtggtg tcttcgctgg
     3061 ccgttgcggg aggcttcctg ctgttcgccc tgccgggtgc agagcccgcc ccttactgga
     3121 cggcgttctt ccccgcgatg gtcgtgcagg gtttcgggat ggcgctggtc ataaccccgc
     3181 tcaccgcggt ggcgctcggc tcggttgagg gagagcactc aggggtggcc tctggggtga
     3241 acaacgccgc ggctagggta gcggggcttc tggcggtcgc cgtcctgggt atgttcgcat
     3301 acgggatgtt ctccgcgaac ttagacgccc gcttagatag catgaacctg cctggagagg
     3361 ttcgtagcga gatggaggcg gcgaaggccg acctcggggc agcagaggca ccggaggggg
     3421 tggacgcggg aacggcggcg cgcatcgagc gcgccgtcga cgagtccttc gtcgccgggt
     3481 tccgcgccgt gatgctggtc tctgccgggc tcgctttcgc cagcgcgctg acggcggtgc
     3541 tgttggttgg taattggaga gtccgatcgg tgagcaggga ttcccatcca tcgggtgggc
     3601 ttcgggcgcg cgtccgacgc cacatccatc atattgcgta gtccatttgc acttcgcttt
     3661 gttggcaccc gggcagagga gccttcgctg taggtcttgg gaggcgttct caggggtccg
     3721 tttgcagcgg aggagcgcac gatcgggacg atgcgcggcg tattgccgaa gccattagga
     3781 aacggcgtcc cgagaatacc gcccgagcag cgagacgaac tagcggtgga gcgagtggat
     3841 gaagctgatc atgaaggtac gcccgaggag tgcgtggaag gggattctag gaagttcgcg
     3901 tgtaggctgt tcccggaagg gcctcctatt gctaagcctc acggggacgg tcccccggat
     3961 gagtaatcgc gagatcgcgg agatgcatgc gctgatttgt gccgttattc tgatggagcg
     4021 accctcggga aggagatgct gcggtgccgg acaccctcga cgacttcgcc gcacgggagc
     4081 cttcctcggc ggaggccgga cgctcccgac cgacgcggct cattgttgcg accaacgtcg
     4141 tgctcgcggg actcctgtca gtcttgtctt tcgccggggt cacggaagtt acgggcatca
     4201 gcgcttatct tggcaccttc tttctcgtag gcgcactcgt tccgtctaac ctgctgcgct
     4261 tcgagcggta cgctagtgga cgtagaaccc gggccctggc aagactccgc aaacctctgg
     4321 gcatcagcgc gggtgtgtgg ttcgtggcgc attctgtcgt gagcgtttgg gagctgttcg
     4381 acctttcgct gccgctcttg ccgcagttca ccagaacggg catcgtcctc ggcctcctcg
     4441 cgacggtggt gttcgtcgcc atgctcgcca cctctaccga ccgagcgcag cgggcgctgg
     4501 gcaggaactg gaagcggctg caccgcctgg tgtggttcgc cgtcccgctg gcgctcgcgc
     4561 acaccatcct ggcgagcggg ttggattcgc cgagcatagt attttatctg gggatcatgg
     4621 ccttcgcggg gttcgagtac cacgccttac ggaggcgcag aatgcggggg gcgcggatac
     4681 acattatgct tatcggcgca ggggtgttcg cggcggcctt gatctacgtg gtgctgtagc
     4741 agcatcgtta ggggcaacaa ccggggcgac ccctcaacgc agacacaggc gctgttcagg
     4801 tatgtcaata aagacttgaa caaccgtctg atggcaagcc ctcttctcaa cctctagtca
     4861 cccaagtgcg tggaggggtt attctgcgaa cttcgccttg agagggtttt cggaccttcg
     4921 gcgcctccta aagaccgatc aactgtgtcc gcgacacatg actgtcacgc agctgtcggg
     4981 tctgttacct tgaccgatcg gaggtgggaa ggccggatga tgcaggtgac agccgaagat
     5041 gtcgtcgaac tccacttggg tctgcagaca cgcggcgtcc agctctggac ggacggtggc
     5101 tggggcatcg gcgcgaagac tctctggatt gcaaaactct cgcaaatagc cgaattccta
     5161 agctaaagta tttctgagca tttcaggatg tttactcggg ttgctgcatg gttgctgcgc
     5221 atttagtgtt ccggcggcct aatggaccgg cgggcatggc agaggtcgct agtctcgccg
     5281 aggagtgcga cccggtcccg ccgcggacgc agacgatata ggaaaccggc cccgatcagg
     5341 ttcgccagcg cggcccgcca aaccggcgtc ctgcggtaaa gatcggcgac actcgcgaac
     5401 ggtctttcgt cccgcttgcg gaggattgcg gagagcgccg tccccgagac ccttttcgct
     5461 ctcaaatggt taaagtccag cttgccgacg tcgggtacat aagggcaagg agaagggcca
     5521 gggctgctac tacgccccgg ccctccttcg cgctacgggg cgctaaacct tagaagggac
     5581 atgaccgtta gaagggacca tggcggcggg ctgctcgacg cccgtcaata tgccatcatc
     5641 agggtcggtc gacagggaaa caactgcgga tgtgttgaat cccttgacga tcagccagat
     5701 accaagcgat aactcccaga agaactcggg gatggtcgcg atgaccgacc atagggagac
     5761 tggcgcgtag agcccgagca acattgcgat ccccgaagcg gagaccagcg ggccgccgac
     5821 gagccccaac agggccatgc gccggggcac gaggttcgac gagtacatca ggtagcccag
     5881 caaaatcccg ttcccgaggc cggcaaggat gccgggcccg agcaggaagg tcgaatcgtg
     5941 taccgctacc agagacttgc cgaccgtaag cagcggggcg gcgtctgcgg ccgcggctcc
     6001 cgcaaagccc tgcctcaacg caaccaccga aatgaggctg acgatgccgg cgaagatgat
     6061 ggcggcttcg aggacacgca cggcaacgta gctaagggca accgcttcgt tctgccgctt
     6121 gacgatcggg aacagcacga cggcggtgcc gatgcaggcg agggcggtga tcacctcgag
     6181 gaccgccccc caccgcacga gggtgtcggc gccggcgctg acgatgtagt tggggtcgtt
     6241 cagcacaggg ccgtacatga tgaactgcgc tgggatcgag gcgacgatgg tgaccaagaa
     6301 caacacgccc gtaacccttg cgatcttttg aggattcatc tgaactcctt tccttgatta
     6361 ggtcactatc ttcggtttat cgctcgggtg cggcggtcgt acggtcacct gcttcttgcc
     6421 ccaccgagct ccgggcacct tgaaataatg ttcgatcacg tagccccaag gcatcacgag
     6481 cggaaccaac accacgccca ccagacaggc aatcagcgtt tctgtccccc cgaagcttac
     6541 gttcggatcc agaccaccgg aaagcaatag gggcagcccg aaaagcagga tccagatggc
     6601 cttccacaca aactcgaaga aaagcagcgg cagcatcttg atcgggtaac ggatccccac
     6661 caccgccaac agcgcggtcg cgccgagcac gcttaggatc acgctgtccc cgggtgagag
     6721 ggtcgcggga tggagtagcg caggcaactt ctcgatcgcc aaaccgacga aggtgaacac
     6781 gtacatggcc ctcaacagat agagcctgaa ggtcgatact tcggacatag ggagtgcact
     6841 cctttccttt agcgaggtca atgacgggcg actaccaacc gacgtggcgg cttcgcccac
     6901 gctcgcacag cgtcacgctc cagccaatca accacctcct ctccgccgcc ggaacaccgc
     6961 gaccaagtct ccaccttatt gcagcttgtc cacgccatgc agcctccagc gaacttacgc
     7021 ggccgcggtt tggtggagct ggggggttcc cgtgccaggt gcgggttccg ccgaggcttg
     7081 ctcgcgccgt tcagaccaga gcgcgtagcc taggcaggcc agcgctatcc ccatcggcac
     7141 cgccgcgtac cgctcgagcg cgtgcgggag cagcgcaacc gcgacgggcg ccacgggcgc
     7201 tgcgacggcg agcaggccgg ccgcccaccg cggcaggatg ccggcgcgga gcgccgcgat
     7261 gccaaatacc aggccgccga gcagatacgc tcccccagtg aggccataga ccgccgggag
     7321 ggcgccgagg tctatctcgc cggggtgccc attgaagatt cccaggaagc cctccacaaa
     7381 cctcggtgac tcggtcgcca accgcggcga gatgaaggct tcggcgaatt ggaaggccat
     7441 ggtcagcgcg tagaagaggc tgaacagaag gtagccggcc agaccaagcc aaccggccgc
     7501 ttccacctgc ctggcgtaga gccccgccag gccgagcagg ccgaagaggc acatggcgac
     7561 gcccagatag tggacgatgg cccattgggc ggtggtgacc gacgccggga catcgggggg
     7621 atggatcggc tggatgacca cgaaaaggat ccctgccacc atagctgcta accctgccca
     7681 acggatgagt ttcggggctg ttatcttcat tttgctctcc ttttgatgtg tagttgcttg
     7741 tgcctttttg atgactgcgg cctggcgatt ggtgttaatg agtttgtctc ctttttcgaa
     7801 cgaacaacga cacgttgagg ggtccgaagt gtctgctggc ggtcggcggt cacgctacag
     7861 tcgccttcgt cgcgacagcg gtgcttgacg ggactatcgc cgccgatgtg tccgcactcc
     7921 ccaactcagc agcgggcgcg gccaggatcg gtgccgacgg gttgaaaccc ttgaaggtaa
     7981 gccagaggcc gagcgacagc tcccacagcg cgatcgggat gaccgcgatc cccgacaaca
     8041 cggaaacctg ctcgttgaag ccgagaatcg atgcggctgc ggagattagg aacacagggg
     8101 ctccgatgag tcccatcgca ggaatgaaac gtggcacgag tcgcgaccgg tacatcaggt
     8161 agcccagcaa cagcgcgttc aggcccggaa tgacgccagg tccgagcagg tgcgccccat
     8221 cacggaccgc gacgagcgcc cggcccacag cgaccagcgc ggccccgtct gttccggcac
     8281 ctcctgcctg ccgcagggtc accaccgcaa ggaggctcac gacgccgata acgatgacgg
     8341 cggcttcgaa caagcgagtg gtgacaaagc caagcgcgaa gccctcgtgc tgtcgtttga
     8401 ccaccgagaa cagcgcgccc gcggtgccga tgcaggcgag ggcattgacc aggtcgagga
     8461 agacgccgag gagcacctgg ttgtcggcgc cggagctgac gatgtagtcc ggattgtcca
     8521 ggacggggcc aagcaggaag gctgcagggg ggatgccgac gatgaagata atcaggtaga
     8581 ggaccccccg acgaacgcgg tcttcctcat cgagtccatc gggacgcgtt tcgttgctgt
     8641 ggtcctaacg gccattctgt ctgtattcat tttttcgttg gtagtcatcg ttcgttctcc
     8701 tctctcaatt cactgggtcg gcggacgggg tgccggtcat cttccaggta ggggcgatgc
     8761 ggctggagcg gtcgaagagc cagatcgcga gccaggcgat tcccacggca aggatgacga
     8821 gtccgaggtc cagcaccgcc tcgggcacga aggctcgcag cgagatcggg acggcgccga
     8881 cggcgtacag gacgagcggc gccgtgggga gctcgcgggt gcggagcaat gcggtgacga
     8941 acgcaagggt gccgaggagg aacaggatgg atgccgaggt cagtgcgata cccagcgagc
     9001 cgttcaggag tgcctcaacg ggaatgacaa cctctgcgag gaggttgatc gcggggatgt
     9061 agatgccgcc ctccggcaag acgaaggagg cgagcggggt ggcccacacg acgatggatg
     9121 cgaccaggct cgcgaggaag atcatcccga atgcgatcca ccagacgttg aggcccagcc
     9181 ggtgcagccc cagcgacttc caaccctccc tggaataccc atcacgggtg accacgagca
     9241 gcatgatcaa tgtcgccacc acaggagtaa tcgcccacac ggaggcccac attagcatgt
     9301 tcgaaccgag gtcgagcatg ttgatcgaca aggccgcccc cacgctcagg aacagcacga
     9361 gcaccgtgaa gatggccgcc ttgctcgtat cggttagctt cgtgaacatg gcctagaacc
     9421 cctcctcccg catccggttg ccggtcttcc tttggttcac gcggggacgg gcttgacggc
     9481 cccagaaggc gtagctgtcc cgggctcctc ggctccccct tcctcctcct gcttgtggag
     9541 cttgcgagaa aggtgcttcg gtccggccca catgaccacc gctaccgcca ccgcgcacca
     9601 caccgccgcc agcagccacg actgcctcac cgagtccgcc cccgagaagg cgagcggcga
     9661 gatgaagctg cccgagacgg tgttgaacat ggcgtggaag agcatgacga tcagcacgct
     9721 gccgccggtg ccgttgtaca cccatgtgag cacgagcgtc caggccagga tgatcacgat
     9781 gtcccacccg cccatctgcc cagtcgcaac aacaagcggc aggtgccaca gggcccacac
     9841 cgcccccagg atcaggctag cgacgagcgc cgagcggccg gcttgcaggc tcgggagggc
     9901 gaaccccctc cagcccggct cctcccatgt cccgccggta ccggggatta ggagtacgac
     9961 ggcgaaggtc gagaagagac ccgtccagcc gcccagatcg gccgcggagg gggcctgggc
    10021 gccgagcaag a
//
LOCUS       DBNGVD010000379         1583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1183745, whole genome shotgun sequence.
ACCESSION   DBNGVD010000379 DBNGVD010000000
VERSION     DBNGVD010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1183745"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1126)
                     /gene="menC"
                     /locus_tag="AC16CM_10250"
     CDS             complement(44..1126)
                     /gene="menC"
                     /locus_tag="AC16CM_10250"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR3140254.1"
                     /translation="MRLAGFDVFRYSLPLRGPVVLGGTAFYRREGLLLRLSGDDGSEG
                     WGESAPLPGFSFESLHLATAQLRGVAGALLGRVVTGDWTGREGGELGRELERFAPAPS
                     VRFGVELAVWNLRAASSGGSLPRLLAGRPRVPVNGLLSGSRDEVLQEARHMKDAGYET
                     VKLKVGAKAAAEDARLVSRLVEELGTSVSLRLDANRAWGYEQAAEFLGGVPHFEYLEE
                     PLADPGRLPQLVREFGVPVALDETLVGMGPGVLGEHRYARAVVLKPTLLGGISNTLRI
                     AERALDLGMRPVVSSSYESGVGTAALVALASGIGERPVPAGLDTYRRLAEDVLDMPLG
                     LPAPSVDVGEAAVAARTVDVGRLQVL"
     gene            complement(1131..>1583)
                     /locus_tag="AC16CM_10255"
     CDS             complement(1131..>1583)
                     /locus_tag="AC16CM_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3140255.1"
                     /translation="DIRAGIIEGDAAVEAASGDVELGEVGGGLSANTAAGDVRLGRLG
                     GRATVRTASGDVQLGTVLAALSASTQSGDVEVEDYEGGDLECNSTSGDVRIGLPSGRT
                     LDIDLNTLSGDIRSDFSPEGGDGATARLRVKTISGDITLLRATGISAG"
BASE COUNT          261 a          589 c          503 g          230 t
ORIGIN      
        1 tgcccgcggg gaccagcata tcctgcaccc aaacggacta gccctagagt acctgcagcc
       61 ttccaacgtc aacggtgcgc gcggcgacgg ccgcctcacc gacatccacg ctcggggccg
      121 gcaggccgag cggcatatcg agaacgtctt ccgcgagcct gcgatacgta tcgaggccag
      181 ccggtacggg acgctctcct atccccgatg ccagcgcgac gagggctgcc gtcccgacgc
      241 cgctctcgta tgacgagctg accacgggcc tcatgccaag gtccagggcc cgctctgcta
      301 tgcgcagggt gttcgagata ccacccagca acgtaggctt gagcaccacg gcccgcgcgt
      361 agcggtgttc cccgagtaca cccggcccca tcccgaccag cgtctcgtcg agggccacgg
      421 gcacgccaaa ctcccggacg agttgcggca ggcgcccggg gtcggcgagc ggctcctcga
      481 ggtactcgaa atgcggcaca ccacccagga actcggccgc ctgctcgtag ccccacgccc
      541 ggttcgcgtc gaggcgcagg gagacggacg tgccaagctc ttcgaccagc ctgcttacga
      601 gcctggcgtc ctcggcggca gctttggctc cgactttgag cttgacggtt tcgtagccgg
      661 cgtctttcat gtgcctggcc tcttgcagga cctcgtcccg ggagcccgag agcaggccgt
      721 ttaccggcac cctcggtcgc cccgccagaa gcctgggcaa cgaacctccc gaagaggccg
      781 cgcgcaggtt ccagaccgcc aactccaccc cgaagcggac agagggtgcg ggggcgaaac
      841 gttcgagctc ccggccaagc tcccccccct cacgcccggt ccagtcgcct gttacaacgc
      901 ggccgagaag ggcccctgcg acgccgcgca actgcgcggt agccaggtgc aggctctcga
      961 agctgaagcc ggggagcggg gcgctctcgc cccagccctc cgagccgtcg tcaccggaca
     1021 gcctcaggag cagcccttcc cgccggtaaa aggcggtacc tccgagcacc acgggtccac
     1081 ggagcggcag gctgtagcgg aagacgtcga agccggcgag cctcatcccg tcagccggcg
     1141 gagatcccgg tggccctgag gagggttatg tctcccgaga tggtcttgac gcgcaggcgc
     1201 gcggtggcgc cgtcaccccc ttcgggcgag aaatcgctgc ggatatcccc ggagagcgtg
     1261 ttcaggtcga tgtccagcgt acgcccggag ggaagcccga tcctcacgtc cccggaggta
     1321 gagttgcatt ccagatcccc accctcgtaa tcttcgacct cgacgtcgcc gctctgggtg
     1381 gaggccgaaa gggcggcgag cacggtcccg agctgcacgt cccccgaagc cgttctcacg
     1441 gttgcccgtc cacccaaccg ccccagcctc acgtcgcccg cggccgtgtt cgcggagagg
     1501 ccgccgccga cctcaccgag ctcgacgtcg ccggaggcag cctcgacggc ggcgtcgccc
     1561 tctatgattc ccgccctgat gtc
//
LOCUS       DBNGVD010000380         3130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3187984, whole genome shotgun sequence.
ACCESSION   DBNGVD010000380 DBNGVD010000000
VERSION     DBNGVD010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3187984"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..460)
                     /locus_tag="AC16CM_10260"
     CDS             complement(92..460)
                     /locus_tag="AC16CM_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140256.1"
                     /translation="MGKKAHRKAARRLAIRFAWRYLVGKKSSYSILSFLSVGSILSAG
                     SILSIGSVGSVLSIGSSGSILSIGSAGSILSIGSSGSILSIGSAGSILSIGSAGSILG
                     IGTAGRGPSGQQEEGSPEEP"
     gene            complement(553..1443)
                     /locus_tag="AC16CM_10265"
     CDS             complement(553..1443)
                     /locus_tag="AC16CM_10265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3140257.1"
                     /translation="MTDYGRDLEFGVSVVPLADPPDWAARIARAADRSGLDLVGIQDH
                     PYQRRFLDTWTLIATLVPVTENVRFFPDVANLPLRPPVMLAKSAASLDLLSGGRIEMG
                     LGAGAFWDAVAAMGGPRRSPGEAVRSVEEAIEVMRLVWSDERSLRFDGEFYSLQGMRP
                     GPQPAHDVGIWVGAYGPRMLDLIGRLADGWVPSLGYAPPERLPGMHGRIDEAAEKAGK
                     DPGDIRRAYNISGKIDEEGDGPLDGPASRWIETLTGFAVEQGMDTFIFWPSEDHERQT
                     GIFANEVAPAVSEAVRKARA"
     gene            complement(1440..2891)
                     /locus_tag="AC16CM_10270"
     CDS             complement(1440..2891)
                     /locus_tag="AC16CM_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140258.1"
                     /translation="MSAEAATTISDEEHSRNTRLLLIVVVTAILVSVLNQTFVNVVVP
                     DIQSDYGVGQGQVGWVITAYLLVFAVGIPLYGRISDLYSLRRTFAVGLAILALGSLFC
                     ALAPTLPLLVAGRIVQGIGAAAIPALGFAAVAKSLPPGERGTALGLLSASVGAGAAIG
                     PVAGGLVAGLVGWHWLFVLTAVMSVGLIPGALRALPDASKAERVGFAEVLRRFDVPGG
                     LALALGAGFALLGVTEGQVRGFGSPASWGSILLSVLFAAFFVWRTRTARIPFVSPHLF
                     RNRAFLSTSAVGFFMMFANVGSLILSPLLLSEENGLSAAGIGLVLAPGALTVAVLSPL
                     TGKLSDRIGPRALVRIGLVIMLVSTVAVSAFAAGAPALWVSAGLWGLGLGFAAVNSPN
                     ANAATATLSPEESGVGLGIYQMIFFLGGGFGPAVAATFLSFRSTPGTDALNPLYALDA
                     AAYSDSFLLVSAATIMAFVASFGLKKATGKQTP"
     gene            complement(2888..>3130)
                     /locus_tag="AC16CM_10275"
     CDS             complement(2888..>3130)
                     /locus_tag="AC16CM_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140259.1"
                     /translation="ISTAGGEQAAANANAAMVNVVHSLRGIVAPLTVGVTRARQRTDD
                     WGEITDENFGRRLDSLGTLVADLAKMVASGRVEAVA"
BASE COUNT          571 a         1078 c         1021 g          460 t
ORIGIN      
        1 cgcacgcccc gcctcgccac ggcgacgaca gaggcggggt cgaggtcttc taccctgacg
       61 gaccctttgc cccggttctg cgtacagcgc atcagggttc ttccggggaa ccttcttcct
      121 gctgcccgga ggggccccgt cccgctgtac cgatgccgag gatagaccct gcgctgccga
      181 tgctcaggat cgatcccgcg ctgccgatgc tcaggatcga tcccgaactg ccgatgctca
      241 ggatcgagcc cgcgctgccg atgctcagga tagaccccga acttccgatg ctgagcacgg
      301 accccacgct ccctatgctc aggatggagc cagcgctaag gatgctgccc acgctcaaga
      361 agctcagaat gctgtacgaa ctcttcttgc cgaccagata cctccaggcg aaccggatcg
      421 cgagccggcg tgcggccttg cgatgcgcct tctttcccat aaggcctcct cgcgctagac
      481 tgctccatca gtctagcagc acgcccggta gcccggggcg agttcgaaac ccgccccgac
      541 agggggcaac gtttacgctc gggcttttcg gacggcctcg ctcacggcgg gagcgacctc
      601 gttggcaaag atccctgtct ggcgctcgtg gtcttcggag ggccagaaga tgaaggtgtc
      661 catgccctgc tcgacggcga agccggttag cgtttcgatc catctggagg cggggccatc
      721 caacgggccg tcgccctcct cgtcgatctt gccggagatg ttgtaggcgc ggcggatatc
      781 ccccgggtct ttgcccgcct tttcggcggc ctcgtctatc ctgccgtgca tccccggcag
      841 gcgctccggt ggcgcgtagc cgagggaggg cacccaaccg tcagccagac gtccgatcag
      901 atccagcatc cgtggcccgt aggcacccac ccagatgccg acgtcgtggg ccggctgggg
      961 gcccggtcgc atcccctgca gcgagtagaa ctcgccgtcg aagcgtaggc tacgctcgtc
     1021 gctccagacc agccgcatga cctctatggc ctcctcgacg gagcgcacgg cttcgccggg
     1081 gctcctgcgc gggccgccca tggcggccac ggcatcccag aaggcaccgg cgccgaggcc
     1141 catctcgatc cggcccccgg agagcaggtc gaggctggcg gcggacttgg cgagcatgac
     1201 cggcggacgc aacggcaggt tggccacgtc gggaaagaag cgcacgttct ctgttacggg
     1261 aacgagcgtg gcgataaggg tccaggtatc gaggaagcgc cgctggtagg gatggtcctg
     1321 gatgcccacg aggtcgagcc ccgaccggtc ggcggccctg gcgatgcgtg cggcccagtc
     1381 gggagggtca gccagcggca ccacgctaac gccaaactcc agatcccttc cgtagtccgt
     1441 cacggggtct gctttccagt agccttcttc agcccgaaag aggcaacgaa ggccattatc
     1501 gtcgcggcgc tcacaagcag gaacgagtcg gagtaggcgg cagcgtcgag ggcgtatagt
     1561 gggttcaggg cgtctgttcc cggagtgctt cggaaagaga ggaacgtggc cgcgaccgcc
     1621 ggcccgaagc cgccgccgag aaagaagatc atctggtaga tcccgagccc gaccccgctc
     1681 tcctccggtg agagggtcgc cgtggcagcg ttggcgttgg gcgagttgac cgccgcgaac
     1741 ccgagcccca gaccccacag gcccgcgctc acccagagcg ccggggcacc cgcggcgaag
     1801 gccgagacgg ctaccgtgga gaccagcatg atgacgagcc caatcctgac gagcgcccgc
     1861 ggtccgatcc ggtcggagag cttgcctgtg agcggcgaga ggaccgctac ggtcagagct
     1921 cctggcgcaa gtacgagccc gatgcctgcc gccgataacc cgttctcctc ggagaggagt
     1981 agcggcgaca ggatcaggct acccacgttg gcgaacatca tgaagaagcc aacggccgac
     2041 gtggagagga aagcccggtt ccgaaacagg tgcggtgaca caaagggtat gcgcgccgtc
     2101 cgggttcgcc acacgaagaa ggctgcgaac aagacggaca ggaggatgct gccccaggag
     2161 gctggggacc cgaaccccct gacctggccc tcggtgacgc ccagaagggc gaaccccgcg
     2221 ccgagcgcga gtgctagccc gccgggcacg tcgaagcgcc ggagaacctc cgcgaaacca
     2281 acccgctcgg ccttcgaggc gtcgggaaga gcgcgcaggg cacctggtat taggccaacc
     2341 gacatcaccg ccgtcagcac gaacaaccag tgccatccca cgagcccggc cacaaggcca
     2401 cccgccacgg gaccgatagc ggccccggcc ccgacgctcg ccgacagcag gcccagcgcc
     2461 gtaccgcgct ctcccggcgg caaggatttc gccaccgccg cgaagccaag ggctggtatc
     2521 gcggccgccc cgatcccctg cacgatgcgc ccggctacga gcaggggcag ggtgggggcc
     2581 agggcgcaaa agagcgagcc cagagcgagg atggcaagac caaccgcgaa cgtgcggcgc
     2641 aggctgtaga ggtcggagat gcgaccgtac aacggtatgc caacggcgaa gaccagcagg
     2701 taggcggtga tcacccaccc cacctgaccc tggccaaccc cgtagtcgct ctggatgtct
     2761 gggaccacca cgttgacgaa cgtctggttt agaacggaga cgaggatggc cgtaaccacg
     2821 acgatgagga gcagccgggt attcctcgag tgctcctcat cactgatcgt ggttgccgcc
     2881 tcggcgctca cgccaccgcc tcgacccgcc cgctcgccac catttttgcg agatccgcga
     2941 cgagcgtgcc gaggctatcc agccgtctcc cgaaattctc atccgtgatt tcgccccagt
     3001 cgtcggtgcg ctgcctggcc ctggtgaccc cgaccgtgag cggcgcgacg atgcccctca
     3061 gcgaatgaac cacgttgacc atggcggcgt tcgcgttggc ggccgcctgc tcgccacccg
     3121 cggtcgagat
//
LOCUS       DBNGVD010000381         3518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1730389, whole genome shotgun sequence.
ACCESSION   DBNGVD010000381 DBNGVD010000000
VERSION     DBNGVD010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1730389"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="rpsF"
                     /locus_tag="AC16CM_10280"
     CDS             <1..355
                     /gene="rpsF"
                     /locus_tag="AC16CM_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3140260.1"
                     /translation="TRPEEANIDTYEAMLIVIPELDEEQVENTIGRFRTIIERTGGEA
                     GEVNHWGRRKLAYEIDHRTDGFYAVMEFTAGDRTLAELKRILRVSDDVLRHMVVKLPP
                     SYAHRPLPEPTEATA"
     gene            378..629
                     /gene="rpsR"
                     /locus_tag="AC16CM_10285"
     CDS             378..629
                     /gene="rpsR"
                     /locus_tag="AC16CM_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3140261.1"
                     /translation="MGRRRNGPPRGGRPTKSKPCVFCKENVAYVDYKDYNLLRRFISD
                     RGKVRARRVTGLCPQHQREAASAIKRAREMALLPYVVGR"
     gene            634..1083
                     /gene="rplI"
                     /locus_tag="AC16CM_10290"
     CDS             634..1083
                     /gene="rplI"
                     /locus_tag="AC16CM_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3140262.1"
                     /translation="MQVILKSDVEKIGQRGDIVEVSRGYVRNFLVPRGLAEVATPSKL
                     EEARRTMEESEERDRRLAERAEEIAGILNKSVITIEARTGEDERLFGSVTATNIANAV
                     ERARGIRLDRRRIRLEEPIKALGTHQVPIQIHGETEASVKVIVVPKL"
     gene            1321..2688
                     /gene="dnaB"
                     /locus_tag="AC16CM_10295"
     CDS             1321..2688
                     /gene="dnaB"
                     /locus_tag="AC16CM_10295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3140263.1"
                     /translation="MRSVETGAEASRVPPHDLEAERAVIGAMLVSESAVAAVAERLDA
                     EDFYSEVHRIIYAAMMRLYSRGDPIDQLTLTNELRSINEFERIGGRPYVFQIVESVPT
                     AANAGRYADIVRGKALLRAVIDVGSRITEDAFREPENVSEALDSAEQLIYGVSNKTLR
                     EHLAPIKDLAPSTLEMIQRLYEQEGEVTGVETGFEDLDRLTTGFHKSDLVVLAARPAM
                     GKTAMALNAIWHAAGEKKMPVAIFSLEMSKEQLVQRLISQTTRIPAQALRSGNVKAED
                     WPKLVRGVAEVARAPIWIDDTAGVTLMEIRAKVRRLASQLNTKCEKPISLVVIDYLQL
                     MIGQGNRQENRQQEIAEISRGLKVLARDLDVPVLAIAQLSRAVEARHDKRPLLSDLRD
                     SGAIEQDADMVMFLYRDEYYNPESDDKGIAEVIVGKHRNGPTGKVQLAWLEQYTKFAS
                     LARRS"
     gene            2798..3277
                     /locus_tag="AC16CM_10300"
     CDS             2798..3277
                     /locus_tag="AC16CM_10300"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3140264.1"
                     /translation="MAFGRVEDRVDVGSRAPDFTLRSQSGANVSLKDYLGKSPVVLFF
                     YPRDETPGCTRQACAFRDDYGQFGALDAEVFGISSDSVESHKSFAENNQLPFDLLSDE
                     GGKVRQLYGVPSTLGLMPGRVTYVIDKEGIVRRIFSSQLAVSRHVEEALEALAEISQ"
     gene            complement(3337..>3518)
                     /locus_tag="AC16CM_10305"
     CDS             complement(3337..>3518)
                     /locus_tag="AC16CM_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3140265.1"
                     /translation="GFGEIEGEWGRQAPLGWETTDPEPVGKAVVSLLSDWWPATTGEL
                     IHVDGGYHAMGAPIK"
BASE COUNT          742 a         1056 c         1130 g          590 t
ORIGIN      
        1 cactagaccg gaggaggcaa acatcgacac ttacgaagcc atgctcatag tgatccccga
       61 gctcgacgag gagcaggtgg agaacaccat cgggcgtttt cgcacgatca tcgagcgcac
      121 gggcggcgag gccggcgagg tgaaccactg ggggcgccgc aagctggcct acgagataga
      181 ccaccgcacg gacggattct atgccgttat ggagttcacc gcgggcgatc ggaccctcgc
      241 tgagctgaag cgcatacttc gcgtctccga cgacgtgctg aggcatatgg tggtgaagct
      301 cccgccgagc tacgcgcaca ggccgctgcc ggagcccacc gaggccacgg cctaactttt
      361 gtttctggag gtaatgaatg ggtaggagaa ggaatgggcc gccacgcggt ggccgtccga
      421 ccaagagcaa gccttgcgtt ttctgcaagg agaacgtcgc ctacgtcgac tacaaggact
      481 acaacctgct gcggcgcttc atctcggacc gtggcaaggt gagggcccgt agggtgacgg
      541 gcctgtgccc gcagcaccag cgggaggcgg cctcggccat aaagcgcgcc agggagatgg
      601 cgctgctgcc ctacgtggta gggaggtaga accttgcagg tcatactcaa gagcgacgtg
      661 gagaagatcg gccagcgcgg cgacatcgtc gaggtaagcc gcggctacgt gcgcaacttc
      721 ctcgtgccga ggggcctcgc cgaggtggcg acgccgtcca agctggagga agcgcgccgc
      781 accatggagg agagcgagga gagggacagg cgcctcgccg agagggccga ggagatcgcc
      841 ggcatcctga acaagagcgt gatcacgatc gaggcccgta cgggcgagga tgagcgactc
      901 ttcggttccg tgaccgccac caacatcgcc aacgccgttg aaagggcccg cggaatccgc
      961 cttgaccggc gccgcatccg cctcgaagag cccataaagg cactcggcac ccatcaggtc
     1021 cccatccaga tccatggcga aaccgaggca agcgtcaagg taatcgtagt ccccaaattg
     1081 taggaacgag gcaaacgcaa cggggatcgg ctccataccg atccccctgt gcacggctcg
     1141 caagacatta gccggtaaga cgccgaatcc tgcaggggtg gttagagaac cgcccgacca
     1201 cgatacgctt aaccttcgat gttaggtgca cccccaatct cgactacaag gtcaaggctc
     1261 ccgtcttgta acgggggcct ttttgctgca tcctggtagg ttatggaacg tcgggggagg
     1321 atgcgcagtg tggagaccgg ggcggaggcg tcccgggtac cgccgcacga cttggaggcg
     1381 gagcgggctg tcataggggc catgctggtt tcggagtcgg ccgtggcggc ggttgcggag
     1441 cgtctcgacg ccgaggactt ctactccgag gtgcacagga tcatctacgc ggccatgatg
     1501 cgcctctact cgcgtggaga ccccatagac cagctcacgc tgaccaacga gctcaggagc
     1561 atcaacgagt tcgagaggat aggcggaagg ccatacgtct tccagatcgt cgagagcgtc
     1621 cccactgcgg cgaacgccgg caggtacgcg gatatcgtgc gcggcaaggc gctcctgcgg
     1681 gccgttatag atgtcggaag ccgcatcacg gaggacgctt tccgggagcc cgagaacgtg
     1741 agcgaggcgc tcgactcggc tgagcaactc atctacggcg tctcaaataa gacgctaagg
     1801 gagcacctcg cgccgatcaa agacctcgcg cccagcaccc tcgagatgat ccaacgcctc
     1861 tacgaacagg agggcgaggt aaccggcgtt gagaccggct tcgaagacct cgaccgtctc
     1921 accaccggct tccacaagag cgacctggtc gtcctcgccg ccaggccggc aatgggaaaa
     1981 acagccatgg cgttgaatgc tatttggcac gctgctggcg agaagaagat gcccgttgcc
     2041 atcttctcgc tggagatgag caaggagcag ctcgtccaga ggcttatctc gcagaccacg
     2101 cgcattccgg ctcaggcttt gaggagtggc aacgtaaagg ccgaggactg gccgaagctg
     2161 gtgcgggggg ttgccgaggt cgcccgagct cctatctgga tcgacgacac cgcgggcgtt
     2221 acgctcatgg agattcgtgc caaagtaagg cgccttgcca gccagctcaa caccaagtgt
     2281 gagaagccga tctcgctcgt ggtcatcgat tacctgcagc tcatgatcgg tcagggcaac
     2341 cgtcaggaga accgccagca ggagatagca gagatcagcc gcggcctcaa ggtcctggcc
     2401 cgagacctcg acgttcccgt cctcgccata gcccagctct cgcgtgccgt ggaagcccgc
     2461 cacgacaaac gcccgctcct gtccgacctt cgggactcgg gcgccatcga gcaggacgca
     2521 gacatggtga tgttcttgta tcgagacgaa tactacaatc ccgagtccga cgacaagggc
     2581 atcgccgagg tcatagtggg caagcacagg aacgggccaa cgggcaaggt ccagctagcc
     2641 tggctggagc aatacaccaa gttcgcctcc ctcgccagga gatcgtagcg agagccggag
     2701 cggctgcgat ccggcgtctt cgtcgacgct cttacaaaaa gctgcacggc acatcgtaga
     2761 atcggttcgt tcgacgagcg ttatcgaagg agatcaattg gcgtttgggc gggtcgaaga
     2821 cagggtggac gtgggttcca gggcgccgga tttcacgctg cgttcccaat caggggcgaa
     2881 cgtgagcctc aaagactacc tgggcaagag ccctgtcgtt ctcttcttct accccaggga
     2941 tgagacgccg ggttgcacca ggcaggcctg tgcgttcagg gacgactacg ggcaatttgg
     3001 cgctctcgat gccgaggtct tcggcatcag ctccgattcg gtggagtcgc acaagagttt
     3061 cgccgagaac aaccagttgc cttttgacct cttgagcgat gagggcggaa aggtgcggca
     3121 actctacggc gttccaagta ccctcggcct aatgcccggc agggtgacct acgtcataga
     3181 caaagaaggc atcgtgagac gaatattctc gtcccagctt gcggtgtcca ggcacgtaga
     3241 agaagcgctg gaagccctgg cggagatctc acagtagatc tgcgccgacc gtcttcaccc
     3301 gtggggtgct gaaagctgat ggctaacagc tgagagctat ttaatcggtg cgcccattgc
     3361 atggtatcca ccgtcgacgt ggatcagctc cccggtggtg gccggccacc agtccgagag
     3421 caggctgacg acggcctttc cgaccggttc ggggtcggtc gtctcccagc caaggggggc
     3481 ctggcgtccc cactcgccct cgatctctcc gaagccgc
//
LOCUS       DBNGVD010000382         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2643442, whole genome shotgun sequence.
ACCESSION   DBNGVD010000382 DBNGVD010000000
VERSION     DBNGVD010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2643442"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="AC16CM_10310"
     CDS             complement(<1..1393)
                     /locus_tag="AC16CM_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3140266.1"
                     /translation="MQREGHNGHRERYETREHDVLIIGAGGAGLRAAVAAAEHGLSVG
                     IVTKSLLGKAHTVMAEGGIAAAVGNVDPDDSWHQHFIDTMKSGKFLNNWQMVETFTKE
                     APQRVYELEQWGALFNRTPEGKISQRPFGGHTYRRLCHVGDRTGLELIRTMQEKVLAT
                     DAEVYMETTITKLFKKEGRVVGALAYTRENGKFVHFKAKAVIMATGGWGRIFKVTSNS
                     WEGTGDGVVLGYDAGAELVDMEMMQFHPTGMVWPPGVRGLLVTEGVRGEGGLLRNSEG
                     ARYMENYDAEKMELSTRDVVARANYTEVQEGRGSEHGGVYLDITHLGYDGIMKKLPTM
                     YEQFLKLADIDISREPMEVFPTVHYTMGGIKIHPETAASSVPGLFAAGEVAGGLHGAN
                     RLGGYSLSDLLVFGRRAGEGASAYIAESVHSADIDEAEVLGEIDRVLEPLEKKGEGES
                     PYLIQQELQEAMME"
     gene            complement(1410..>2007)
                     /locus_tag="AC16CM_10315"
     CDS             complement(1410..>2007)
                     /locus_tag="AC16CM_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140267.1"
                     /translation="WMPGFVTAPMLVLWIPLGFRATCYYYRKAYYRAFFFDPPACQAG
                     AQRKDRFWAGKDAAEGYRGERALFVLNNVHRYFLYASIIVVAFLWYEAFRTLFGPQGF
                     QVSVGSLLWFLNIVLLSAYTFSCHSFRHLIGGNKDCYSCIRGGNTRRKAYNFVSRLNL
                     RHPNWAWYSLFSLVATDLYLRLLQTGVITDFVIIPFGG"
BASE COUNT          341 a          678 c          616 g          372 t
ORIGIN      
        1 gctccatcat ggcctcctgg agctcctgct ggatcaggta cgggctctcg ccctccccct
       61 tcttctccag cggctcgagg acgcggtcga tctcacctag cacctctgcc tcgtcgatgt
      121 ccgccgagtg cacgctctcc gcgatgtacg cggaagcgcc ctcaccggca cgccgtccga
      181 agacgagcag gtcggagagc gagtagcccc ccaggcggtt ggcgccgtgc agcccgccgg
      241 ctacctcccc ggcagcgaaa aggccaggca cgctggaggc agcggtctcg gggtggatct
      301 tgatgccgcc catggtgtag tggaccgtgg ggaagacctc catgggctcc ctggagatgt
      361 ctatgtcggc aagcttgagg aactgctcgt acatggtcgg gagcttcttc atgatcccgt
      421 cgtacccgag atgcgtgata tccagataaa cgccgccgtg ctcgcttccg cggccctcct
      481 gaacctcggt gtagttggcc cttgccacca cgtcgcgggt ggagagctcc atcttctcgg
      541 cgtcgtagtt ctccatgtag cgcgcgccct cggagttgcg cagcaacccg ccctcgccgc
      601 gcacgccttc ggtgacgagg agcccgcgca cgccgggggg ccacaccatc ccggtcgggt
      661 ggaactgcat catctccatg tcgaccagct cggcgcccgc atcgtagccg agcacgacgc
      721 cgtcaccggt cccctcccag gagttggagg tgaccttgaa gatgcgcccc cagccgccgg
      781 tcgccatgat gacggccttg gccttgaagt gaacgaactt tccgttctcc cgcgtgtagg
      841 ccagtgcccc gacgacccgc ccctccttct tgaagagctt ggttatggtc gtctccatgt
      901 agacctcggc gtccgtggcg agcaccttct cctgcatggt ccggatcagc tccagccccg
      961 tcctgtctcc gacgtggcag agccgcctgt acgtgtggcc gccgaagggt cgctggctta
     1021 tcttgccctc gggcgtcctg ttgaagaggg cgccccactg ctccagctcg tagacccttt
     1081 ggggggcctc cttggtgaag gtctcgacca tctgccagtt gtttaggaac ttgccgctct
     1141 tcatggtgtc gatgaagtgc tggtgccagg agtcgtcggg gtccacgttg ccgacggcgg
     1201 ctgcgatgcc gccctcggcc atcacggtgt gggccttgcc gagcaggctc ttggtgacga
     1261 tgccgaccga gaggccgtgc tcggcggcgg ccaccgcggc cctcaagccg gcgccgccgg
     1321 cgccgatgat caggacgtcg tgctcgcgcg tctcgtagcg ctcgcgatgc ccgttgtgtc
     1381 cttctctctg cataagatac ctctctagat cagccgccga atgggattat cacgaagtcg
     1441 gttatgacgc cggtctgcaa cagcctcaag tacagatcgg tcgccaccag agaaaacagg
     1501 ctgtaccagg cccagttggg atgcctgagg ttgagccggc tcacgaaatt gtaggccttg
     1561 cgccgcgtgt tcccgccccg aatgcaactg tagcagtcct tgttcccacc tatgagatgg
     1621 cggaaggagt ggcacgagaa cgtgtaagcc gagagcagca cgatgttcaa gaaccacaac
     1681 agcgagccga cgctgacctg aaaaccctgc ggcccgaaca gggtccggaa agcctcgtac
     1741 cacaggaacg ccaccacgat gatcgaggcg tacaggaagt accggtgtac gttgttcaga
     1801 acgaagagcg cccgctcccc cctgtacccc tccgccgcgt ccttcccggc ccagaagcgg
     1861 tccttgcgct gcgcgcccgc ctggcaagca gggggatcga agaagaacgc ccggtagtac
     1921 gccttccggt agtagtagca ggtcgccctg aacccgagcg gaatccacaa gacgagcatc
     1981 ggcgccgtca cgaaccccgg catccag
//
LOCUS       DBNGVD010000383         3581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1913050, whole genome shotgun sequence.
ACCESSION   DBNGVD010000383 DBNGVD010000000
VERSION     DBNGVD010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1913050"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1419
                     /locus_tag="AC16CM_10320"
     CDS             430..1419
                     /locus_tag="AC16CM_10320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3140268.1"
                     /translation="MLISGGRLRALWRLVIQFLTFRVIVAVLFSTLAVTLSLTASGGR
                     SVTMSSPAILLASGVAGLAGAMLSVWLAGRYLDRRPFSDFGFHLGAGWLLDLLFGMLL
                     GGVLISTIFIIELGLGWVEVSGSFETFGTGLSFLPSMLLPVVMYLCVGIYEETVFRGY
                     QLKNAAEGLDYPALGPRGAILVAWILSSVFFGLLHANNPSATAVSTLNIVLAGLLLGF
                     GYVVSGELAIPIGLHITWNLFQGAVYGFPVSGFGPFGATIVETRQTGPDLWTGGAFGP
                     EAGLLAPIAMLLGISLIALWTRLRTGRVALHSPLAEGPNRDPLTRATGDLPEA"
     gene            1493..2734
                     /locus_tag="AC16CM_10325"
     CDS             1493..2734
                     /locus_tag="AC16CM_10325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR3140269.1"
                     /translation="MAGTTLLRNALLIDCAGDGPRERASVVVEGGRISEVGYGKDPAI
                     AGHDAVIDCAGMTLMPGLTDAHVHIGAVDVNILDQHRERPSNLVALMMARILEDTIQE
                     GFTTVRDAGGTDWSFKAAVERGVVQGPRLLVSDKPLSQTGGHGDWRRSTETQPPEIFC
                     PTAGMRSVVCDGADEVRRATREQLRQGADQIKVMASGGAMSPADELASTQYTLDELRA
                     AVEEAEAAGTYVMAHAYNAGSVRNCLSSGVRSIEHGNLIDGETAALIASSGAYLVPTL
                     VTYEALSEEGDAYGVPENVIRKIDEARALGIRALRYAYEAGAKIASGSDLLGPLQNRK
                     ARELEIKTEVMTPMESLVSATRTNAELFGLENEIGTVEVGKLADLLVVDGNPLEDIAI
                     LQDRTNLKLIMKAGEAVKNEL"
     gene            complement(2816..3319)
                     /locus_tag="AC16CM_10330"
     CDS             complement(2816..3319)
                     /locus_tag="AC16CM_10330"
                     /inference="COORDINATES: protein motif:HMM:NF018765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HIR3140270.1"
                     /translation="MKHRQPSAGTFEGYKKGADVPLLSYSVLAGTFNLLFALFLLATR
                     ASGKTLPPRIEARDIALLGMATHKLSLVGSQDAITSPIRAPFTELQEKESPKKVDEKP
                     RGEGIRRSVGELLTCQFCLGVWVASLFTYGFVLLPRVSRLIASVFAAVTVADFLHQIY
                     KILKKSA"
BASE COUNT          666 a         1122 c         1174 g          619 t
ORIGIN      
        1 cctattcaag gacaggttcg gtgtgtgggg ccgctccggc aaaaaggcct tcaacgagac
       61 cgatgagttt cagcggatgg tacgccacta catgaaagcc atcgacgaca cggtcgagct
      121 caggcgtcag aacgcctgag ctcggcacgc tgcggcctgt ggcctccgct tgtcagcccg
      181 cttcgggccc tacgggccct cgctgtcagt caggccccgc tgcggcgcaa ccccttccgc
      241 acgcgaggta gggtatgaag agaaggccac atgccgggct cgccgtcacg gacagagacg
      301 accggctgaa agcgaggcga agccgaagcg ttgccgacaa gcgacagcct ccaggctgga
      361 gcgtgctgac aagctggcgt agcggagcgg agccggcgtg ctgacaagcg aagcccttca
      421 gggcgaagcg tgctgatctc cggggggcgt ctcagggcac tctggcggct ggtgatacag
      481 ttcctcacct tcagggtgat cgttgccgtg ttgttcagca cgctcgcggt gaccctgtcg
      541 ctcaccgcct cggggggcag atccgtcacg atgagctcgc cggctatact gctcgcgagc
      601 ggggtggccg ggctcgcggg ggccatgttg agcgtgtggc ttgccggtcg ctatctcgac
      661 aggcgtccct tctccgattt cgggttccat ctcggcgccg gatggttgct cgacctgctc
      721 ttcgggatgc tacttggcgg cgtacttatc tcgaccatct tcatcatcga gctggggctc
      781 ggctgggtcg aggtctcggg atccttcgag accttcggga cgggcctctc cttcctgccc
      841 tccatgctcc tgccggtggt gatgtacctg tgcgtgggca tctacgagga gacggtcttt
      901 cggggatacc agctcaaaaa cgcggcggag ggcctcgact acccggcgct cggcccccgc
      961 ggcgctatcc tggtggcctg gatactgtct tctgtctttt tcgggctgct gcacgccaac
     1021 aacccaagtg ccacggccgt cagcacgctc aacatcgtgc tcgccggcct gctgctcggc
     1081 ttcgggtacg tggtctcggg cgagcttgcc atacccatag ggctgcacat cacctggaac
     1141 ttgttccagg gcgccgtcta cggctttccc gttagcggct tcgggccgtt cggcgccacg
     1201 atcgtcgaga ccaggcagac cggcccagac ctgtggaccg ggggggcctt cggacccgag
     1261 gcgggcctgc tggccccgat cgccatgctc cttggcatct ccctgatagc cctgtggact
     1321 cgcttgagaa cgggcagggt cgcgctccac tctccgctcg cagaaggccc gaaccgggac
     1381 ccgctaacca gggcaacagg tgatcttcct gaagcctgaa acatgtagcc tgtagccctc
     1441 ttcccgcgga taggccatac ttttcgagat cgttctgttg aaaggggaga aattggctgg
     1501 aacgacgctt ctgaggaacg ctttgctcat agattgcgcg ggtgacgggc cgcgagagcg
     1561 cgcctccgtc gtggtcgagg ggggtaggat ctcggaggtc gggtacggga aggaccctgc
     1621 gattgccggg catgacgccg tcatcgactg cgcggggatg acccttatgc ccgggctcac
     1681 cgatgcccac gtccacatag gggccgtcga cgtcaacatc ctcgaccagc acagggagcg
     1741 tccctccaac ctcgtagccc tgatgatggc caggatcctg gaggacacca tccaggaagg
     1801 tttcaccacc gtcagggacg caggggggac cgactggagc ttcaaggcgg ccgtggagcg
     1861 gggggtagtc caggggccgc ggctgctcgt gagcgacaag cccctctcgc agaccggcgg
     1921 gcacggggac tggcgcaggt ccaccgagac gcaaccgccg gagatcttct gtcccaccgc
     1981 cggcatgcgc tccgtcgtgt gcgacggggc agacgaggtg aggcgggcca ccagggagca
     2041 actcaggcag ggggccgacc agatcaaggt gatggcctcg ggcggcgcca tgtcccccgc
     2101 agacgagttg gcctcgaccc agtacaccct ggacgagctt cgggccgccg tggaggaggc
     2161 cgaggccgca ggtacctacg taatggccca cgcctacaac gccggaagcg tcagaaactg
     2221 cctcagctcg ggcgtgcgct ccatagaaca cggcaacctc atagacgggg agacggcggc
     2281 cctgatcgcg agctccggcg cctacctcgt gcccacgctc gtcacctacg aggccctctc
     2341 cgaggaggga gacgcctacg gggtgcccga gaacgtgatc cgaaagatag acgaggcgcg
     2401 ggcgctcggt ataagggcgc tccgctacgc ctacgaggcc ggcgccaaga ttgcctccgg
     2461 ctctgatctc ctgggtcccc tgcagaaccg caaggcccgc gagctcgaga tcaagaccga
     2521 ggtcatgacc cccatggaaa gcctcgtgtc cgcgaccagg accaacgccg aactcttcgg
     2581 gctggagaac gagattggaa cggtggaggt gggcaagctg gccgacctgc tcgtggtcga
     2641 tggcaacccc ctggaagata tcgccatcct gcaagacagg accaacctca agctgataat
     2701 gaaagccggc gaggcggtga agaacgagct gtaaagctgt cagctatcag caaaaatata
     2761 tagctggccg ctgacggcta atggctgaca gcgcggttcg cgaagcgaac cgcttctaag
     2821 cgctcttttt gagtattttg tagatctggt gcaggaagtc tgcgacggtg acggcggcga
     2881 agacggacgc tatgaggcgg ctgacgcggg gcagtagcac gaagccgtag gtgaagaggg
     2941 aggcaaccca cacgccgagg cagaactggc acgtcaggag ctcccccacc gaccttctga
     3001 tgccttcgcc cctgggcttc tcgtcgacct tcttggggct ctccttctcc tgtagctcgg
     3061 taaaaggggc cctgatgggg ctcgtgatcg cgtcctgcga gccgaccagg ctgagcttgt
     3121 gggtggccat cccgagcagc gctatgtccc tggcctcgat gcgcggcggc agggttttgc
     3181 ccgaggccct ggtcgccagg agaaagagcg cgaacagcag gttgaaggtg cccgcgagca
     3241 cgctatacga taatagtggt acgtcggcac ccttcttgta tccttcgaaa gtccccgcag
     3301 acggttggcg atgcttcata tcggctccct actaataaat acgcggtgta gcgtgcgctc
     3361 tgttggatat ccccccggca tcgtgccgcc taaacatgga agccaggaag cttcctggct
     3421 gtttggggcg gggcaagaaa gggaaaggca gccgaacccc gagatcagga aggacggccg
     3481 aagtgacgga cgaccagcac accaggcaag accccacaac acagtacccg cagccggagt
     3541 acccggagca aacccagatg gaccagcacc ctggcctcga c
//
LOCUS       DBNGVD010000384         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2277244, whole genome shotgun sequence.
ACCESSION   DBNGVD010000384 DBNGVD010000000
VERSION     DBNGVD010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2277244"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..324)
                     /locus_tag="AC16CM_10335"
     CDS             complement(58..324)
                     /locus_tag="AC16CM_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140271.1"
                     /translation="MLLATAKPPPGKAWTRTNSAREEFAGLVGIGGGCEVYMKCRGKG
                     RPTAVLVSGGAGSHEDWTHLIDAEGGLEPSGSAVFPQVGKFTRV"
     gene            complement(504..1094)
                     /locus_tag="AC16CM_10340"
     CDS             complement(504..1094)
                     /locus_tag="AC16CM_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140272.1"
                     /translation="MSVKEISGQVSGRVDPRVVRTRELIVRAFNELVAEKGHTGLTVS
                     EIAGRATINRATFYAHFADQYELFDYVVSEAFREELRRRLPASSGLGEEELRAMVLAA
                     CDYLSGLNTACSRTDRQFKPLIEARVQAELYELLLRWIWASPVNARSADAEITATVVS
                     WAIFGAGLRWSQGGEPGSVEEYADRVLSAIVGGLGS"
     gene            1238..1867
                     /locus_tag="AC16CM_10345"
     CDS             1238..1867
                     /locus_tag="AC16CM_10345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3140273.1"
                     /translation="MDVTIIGTGNMARGIATRLLAGGHGITLLGTEARKAEDLAGELG
                     GSVRAGVVGDEISGDVVVLAVPYEAAVPVLREYGEALSGKTVVDITNPVDFQTFDALV
                     TPADSSAAEEIQKGAPEGVSVVKAWNTTFAGTLVADEVAGQPLDVFVAGDDTESKEAV
                     IGLVESGGSRALDAGSLRRSRELERLGFLGMTLQQPLGLGFQSSWKLVS"
     gene            1898..>2489
                     /locus_tag="AC16CM_10350"
     CDS             1898..>2489
                     /locus_tag="AC16CM_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3140274.1"
                     /translation="MSIVITGASGHLGRATAELVLDRMPASEVILTTRHPEALSDLAA
                     RGAEVRDADFERPETLERAFEGGDKLLLISTDAVGARVEGHRAAIEAAGKAGVRHIIY
                     TSYLNPVEDNPAAVTADHRETEATLRESGLAWTMLRNGLYAEYQVPTGTQAVATGRLV
                     HNNGDGMTAYVSREDCAAAAAAVLGTEGHEGEAYDIT"
BASE COUNT          453 a          799 c          815 g          422 t
ORIGIN      
        1 cggggtcgag ggagacagcg tgccttcgaa acgcagggtt ccgggacgat cgtagactca
       61 gacccgggtg aacttgccga cctggggaaa caccgcggag ccgctgggtt ctagcccgcc
      121 ttcggcgtct atcaggtggg tccagtcctc gtgtgagccc gcaccgccgg agactagaac
      181 ggccgtggga cgtcccttgc cacggcactt catgtacacc tcacagcccc cgccgatgcc
      241 gaccaggcca gcgaactcct ctcttgccga gttcgttctt gtccaggctt tccctggcgg
      301 gggctttgcc gtcgccagca gcatcgtctt cctcagcatg cggcagagga aggtgctgga
      361 gcccccctcc cctgttgcgt aacaccaaga ctacactctg cgtggtgagg gcccttcccc
      421 caaaagggat agggactagg cacgaggacg tttcagagat ctgacctccg cgaagttgga
      481 tgctcgacga attgggtcta gattcaggat ccgagtcctc cgacgatggc cgagaggact
      541 ctatccgcgt actcctcgac gcttcccggc tccccgccct ggctccaccg cagccccgcg
      601 ccgaagatag cccagctgac cacggtggcc gtgatctccg cgtcggcgga gcgggcgttc
      661 acgggcgacg cccagatcca acgcagcagg agctcgtata gctcggcctg aaccctggcc
      721 tctatgagcg gcttgaactg cctgtccgtc ctcgagcagg ccgtgttgag gccagagagg
      781 tagtcgcagg cggcgagcac catcgccctc aattcctcct caccgagccc cgacgaagcc
      841 ggcagcctcc gccgcaattc ctccctaaat gcctcgctga ccacgtagtc gaagagctcg
      901 tactggtccg cgaagtgcgc gtagaaagtg gccctgttta tggtggccct gccggcgatc
      961 tcgctcacgg tgagcccggt gtgacccttc tccgccacaa gctcgttgaa cgcccgcacg
     1021 atgagctcgc gggtgcgcac gacccgcgga tccacccgcc ccgaaacctg cccactgatc
     1081 tccttgaccg acattttcgc tcctctgttg ctttgccaac accaaaccct tcctgatgct
     1141 tgaatccagg ccccccagat agcacaatcc tactcgacag ttgttgtttt agcaacgagc
     1201 gatgtaaaag aaacgtgtga ggcgaaggag gacagagatg gacgtaacga taataggtac
     1261 gggcaacatg gcgcggggca ttgcgaccag gctcctggct ggtgggcatg ggataacgct
     1321 tctcggcacg gaggctagaa aggcggagga tctggcgggt gagctcggtg ggtccgtccg
     1381 tgccggtgtg gtgggggatg agatctcggg cgacgtggtg gtgcttgccg taccctacga
     1441 ggccgccgtg ccggtattga gggagtacgg ggaggcgctc tctggaaaga ccgtggttga
     1501 cataaccaac cccgtcgact ttcagacctt cgacgccctc gtcacccctg ctgacagctc
     1561 ggcggccgag gagatccaga aaggcgctcc tgagggcgtg agcgtggtga aggcctggaa
     1621 cacgaccttc gcgggcacgc tggtggccga cgaggtggct ggccagccgc tcgacgtgtt
     1681 cgtggccggc gacgacaccg agtccaagga ggccgttata ggtctggtcg agtccggtgg
     1741 gtccagggcc ctcgacgccg gttccctgcg caggtcgcgc gagctggagc ggctcggctt
     1801 tctcggcatg acgctccagc agccgctcgg gctaggattc cagagctcct ggaagctcgt
     1861 aagctagagt gtctagaaaa gcggggaggt agagagcatg agcatcgtta tcacgggggc
     1921 atcgggccat ctcggccgtg cgaccgccga gctggttctg gacaggatgc cggcctcgga
     1981 ggtgatcctc acgacgcgcc atccagaggc gctatccgac ctcgcagcac gtggggcgga
     2041 ggtccgggat gctgacttcg agaggccgga gaccctggag agggcgttcg agggtgggga
     2101 caagctcctg ctcatcagca ccgacgccgt cggtgcccgc gtcgaggggc accgagcagc
     2161 catcgaggcg gccgggaagg cgggggtaag gcacatcatc tacacgtcat acctcaaccc
     2221 ggtcgaggac aacccggccg cggtcaccgc ggaccaccgg gagaccgagg cgacgttacg
     2281 cgagagcggc ctcgcctgga ccatgctacg caacggcctc tacgccgagt accaggtgcc
     2341 caccgggacg caggccgtgg ccacgggccg cctcgtccac aacaacgggg atggcatgac
     2401 ggcctacgtc tcacgtgagg actgcgccgc ggctgctgcc gcggtccttg gcaccgaagg
     2461 ccacgagggc gaggcctacg acatcaccg
//
LOCUS       DBNGVD010000385         6310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_457176, whole genome shotgun sequence.
ACCESSION   DBNGVD010000385 DBNGVD010000000
VERSION     DBNGVD010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_457176"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /gene="dnaX"
                     /locus_tag="AC16CM_10355"
     CDS             complement(<1..1353)
                     /gene="dnaX"
                     /locus_tag="AC16CM_10355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3140275.1"
                     /translation="MKHTTLYRKWRPRAFSGIVGQEAVVRTLRRAIETDRVSHAYLFS
                     GPRGTGKTSTAKVLAMGLNCEQGPTAEPDGTCESCRAIVGNSSLDVLEMDAASNRGID
                     EIRDLRDKVNLAPVAGRVKVYIIDEVHMLTTEAFNALLKMLEEPPEHVVFVLATTEKH
                     RVLPTIISRCQSFDFRRPGIETLTEKLAEIAAAEGIEVEPEALTVIAREGRGSFRDAE
                     GLLDQLSSFSEGEISASMVRELLGGVGPEALIETTYALSERRAADALLVVDRLTGEGK
                     DLGQFVVELLAHLRNLMLLPHAPEVALAGVGAEERAPLEEQANAIPTAEVVRLVDALG
                     DALSRVRRGGDPKLELELTFLKLTRDYTEPNLDALLGRLETLEKALENGDPVPVSRPT
                     VEAVEAPPETPRERPRPVEREPEQEVPEPEEPPREEPVRSEPDGGNEPGIDLASGWVG
                     I"
     gene            complement(1605..2294)
                     /locus_tag="AC16CM_10360"
     CDS             complement(1605..2294)
                     /locus_tag="AC16CM_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140276.1"
                     /translation="MKANGGETMIIRSHHRTRWAVLLLAVALAVALVAYAWPAAQRAD
                     AADFKTVTKTLDKDGVIQIPGQFGSQIGPASPYPSGKLVSSFPQGSTILDVDLILRSF
                     THQSPAEIDVMLVHGITKRVVMGDAGSIFDVGDPIRLVLDDEAESKLPQNAQIVEGRY
                     RPANYPTADPFDPPAPQVANGDTTPKLSGFDGKNPNGGWKLFVDDQFNTGTGVILDGW
                     SLRVRAKVPTN"
     gene            complement(2428..5583)
                     /locus_tag="AC16CM_10365"
     CDS             complement(2428..5583)
                     /locus_tag="AC16CM_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3140277.1"
                     /translation="MSNGVPTDIPYVGAAGGSEVRVELLGGFRIRVGRRVVGQDTWRL
                     RKAAALVKLLALAPGCRLQREQVLEHLWSDRPPKAAANNLRQALYVARKVLGADEGGR
                     YLKLQGGLLVLCPEERLWVDARAFEAAAASARRSAREPAAYRAAINLYAGDLLPEDRY
                     EEWTMNRRESLKNTYLSLLVELAEIHEERGEYDPAITALNVAVEAGPSDQWARVALMR
                     AYARSGRHREALLQHVRLREVASRQTDPGLQEAVRRLYEEMLPGDPSDRSGADRSRPH
                     VMPATPSGAGPHNLPAERTSFVGRADQRAEVERVLAGTRLLTLTGTGGLGKTRLALAV
                     ANSLVGSYEDGVWLVELAPRRDPELVAQTVAQALGVREQPGRPLFAALKEYLTSKNLL
                     LVLDNCEHLVDAVARLVESLLDSCPDLRVLTTSRETLNVGGELVWRVPPLTIPETRHP
                     VAVEDLAGIESVRLFVERAALRVPSFALEPGNAQAVAEVCQKLDGLPLAIELAANRVG
                     ALSVAQISTRLEGSLRLLTGSRTAPKRQRTLEGALDWSFELLDEQEKMLFGRLSVFAG
                     SWTLESAEAVGRGTSDSEDCVLELLLRLVDRSLVMVDDGPGDVRYKMLEPVRQYGRQK
                     LEAEGRAAGARRRHATWFLKQAEGAEVEGPRQMVGLERLEMEHDDLRDALAWLAHEGK
                     AERGLHLAATLGRFWYQRGYFTEGISWLETFLRLSAGNGNTAVRAKALYELGALVNRN
                     AGPSSEEREVARTHLRESLRIYRELGDGPCATVLRELGSISIGMGDWDTARSALQDGL
                     ELGRRSGDAVDLARTYTFLGIMACLQGSHDAAQAHFEASLENLGEGGSRSDANTNLFF
                     LGCLACDRDDYALARSWFGEMVQGNSLHVFRWAAPVVLVGYARLAAGEGHAARALRLA
                     GAADTLRRSVGGPTSPTFVAYLRRGLEPSWKELGHNKGASAFDEGRKMSLEDALAYAF
                     EDGEPPSVPRESPAVGSGRTTLTRREREVAVLVARELSNREIADELFVSEHTVATHVH
                     RILGKLGLRSRTQIAAWMIGQ"
     gene            complement(5714..5980)
                     /gene="ffs"
                     /locus_tag="AC16CM_10370"
     ncRNA           complement(5714..5980)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16CM_10370"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         1058 a         2176 c         1919 g         1157 t
ORIGIN      
        1 gatcccaacc caccccgagg cgaggtcgat cccgggctca ttcccgccgt cgggctctga
       61 ccggacgggt tcctcacgag gcggctcttc cggctcgggg acctcctgct cgggctctcg
      121 ctctacgggt cgcggcctct cgcgaggggt ctcaggagga gcttccaccg cttccaccgt
      181 cggccgggag acgggaacgg ggtctccgtt ctccagggcc ttctcgagcg tttcaaggcg
      241 gccgagcagg gcgtccaggt tcggttccgt gtagtcgcgg gtgagcttca ggaaggtgag
      301 ctcgagctcc agcttcgggt ctccgccgcg gcgtacgcgg ctaagtgcgt cgccaagggc
      361 gtcgaccagg cgtaccacct cggccgtcgg aatagcgttg gcctgctcct cgagcggggc
      421 ccgctcctcg gccccgaccc ccgcgagcgc cacttcgggg gcgtgcggta gaagcatcag
      481 gttgcgcagg tgggctagga gctcgacgac gaactggccg aggtccttgc cctcccccgt
      541 caggcggtca accaccagca gggcgtcggc agccctgcgt tcggagaggg cgtaggtggt
      601 ctcgatcagg gcctccggcc ccaccccgcc gagcaactcg cgcaccatgg aggccgagat
      661 ctcaccctcc gaaaaagacg agagctgatc cagaagcccc tccgcatccc gaaacgagcc
      721 cctcccctcc cgcgcgatga cggtcagggc ctcgggctcg acctcgatac cctcggccgc
      781 ggctatctcg gcgagcttct ccgtcagcgt ctcgatgccg gggcgcctga aatcgaagct
      841 ctggcacctg ctgatgatgg tcggcaacac cctgtgcttt tcggtggtgg caagcacgaa
      901 cacgacgtgc tcggggggtt cctcaagcat cttcaggagg gcgttgaagg cctccgtcgt
      961 caacatgtgg acctcgtcga tgatgtacac cttgacccgc ccggcgacgg gcgcgaggtt
     1021 caccttgtcc ctcaggtccc tgatctcgtc gataccgcgg ttggaggccg cgtccatctc
     1081 gagcacgtcg agcgaggagt ttccgacgat ggcccggcaa ctctcgcagg tcccgtccgg
     1141 ctccgccgtc ggtccctgct cgcagttcaa ccccatcgcc agcaccttgg ccgtgctggt
     1201 cttacccgtc ccacggggcc cggagaagag gtaggcgtgc gagacgcggt ccgtctcgat
     1261 ggcgcgcctt agcgtacgca ccaccgcctc ctgccccacg atcccgctga acgcgcgcgg
     1321 acgccacttg cggtagaggg tagtgtgttt cataaggagt atcgtcggtt cgcgtaaatc
     1381 acatttctag tataccggat ggtcaggcgc caaccatcag gcgggttggg tcaccagcat
     1441 cctggcgtcc gtctcctgtg aaggcagaac gtttcaccca gacacgggtc agggcgccga
     1501 cccggagtag ctggaaactc ggcgatagac atggggaagt gccacttgcg ccgacgcgca
     1561 gcaaagaggg gccggacctt caagggtccg gcccgctcgt ggggtcagtt ggtcggtact
     1621 ttcgccctga cgcggaggct ccatccgtct aatatgaccc cggtgccggt gttgaactgg
     1681 tcatcgacga agagcttcca tcccccgttg gggttcttgc catcgaaccc ggatagcttc
     1741 ggggtggtgt ccccgttcgc aacctgcggc gcaggaggat caaagggatc cgcagtcgga
     1801 taattggccg gcctgtatct gccttccacg atctgggcgt tctggggtag cttgctttcg
     1861 gcctcatcat cgagcacgag cctgatgggg tcgccgacgt cgaagataga tcccgcatcg
     1921 cccatgacga cacgtttggt gatcccgtgt accagcatca catctatctc ggcaggggac
     1981 tggtgggtga agcttctcaa gatgaggtcg acgtcgagta tcgtggaccc ctgggggaag
     2041 ctgctcacca gcttacccga aggataaggc gaagcaggtc caatctgcga accgaactgt
     2101 cccgggatct ggatcacacc gtccttgtcg agcgtcttgg tgaccgtttt gaagtcagcg
     2161 gcgtccgccc gttgcgcggc aggccaggcg taagctacca gggcgaccgc aagcgctacg
     2221 gccaacagca gcaccgccca ccgcgttctg tggtggctcc ggatgatcat ggtctctcct
     2281 ccgttcgctt tcatccacac caccctatcc gaagggcgtt agcgaaacgt tagccgcgtg
     2341 gcttcgcaat gtacacatgt tgtgtatttc tgggaatatt cctctctcct taccacggga
     2401 aagtggatct tcgctctacc gaggggccta ttggccgatc atccaggcgg cgatctgggt
     2461 ccgggaacgg agcccgagtt ttccgagtat cctgtggacg tgcgtcgcga cggtgtgctc
     2521 ggaaacaaag agctcgtccg cgatctcacg gttgctcagc tccctggcga cgagcaccgc
     2581 aacctcccgc tcgcgccggg tcagggtcgt ccgccccgat ccgaccgccg gactctctcg
     2641 aggtaccgac ggtggctccc cgtcctcgaa cgcgtaggcg agagcatctt cgagcgacat
     2701 cttgcgcccc tcatcgaagg ccgatgctcc cttgttgtgg ccaagctcct tccaggaggg
     2761 ttccagtcca cgccggagat aggccacgaa ggtcgggctc gtcggacccc cgacactgcg
     2821 ccgcagagtg tccgctgcac ccgccaggcg gagagcacgt gccgcgtgtc cctcgcctgc
     2881 ggccaggcgc gcgtaaccta cgaggaccac gggcgcggcc caacggaaca cgtgcagaga
     2941 gtttccctgg accatctctc cgaaccagga tctggccaaa gcgtagtcgt cccgatcgca
     3001 ggcaaggcac cccaggaaga acaggtttgt gttcgcgtcg ctcctgctcc ccccctctcc
     3061 aaggttctcg aggcttgcct cgaagtgggc ctgcgcagca tcgtgggacc cctggaggca
     3121 ggccatgatg cccagaaacg tgtaggtcct ggcaaggtcg accgcgtcgc ccgatctccg
     3181 gccgagctcc agaccatcct ggagggcgga gcgcgcagta tcccagtcgc ccatcccgat
     3241 gcttatgctc ccgagctccc tgagaacggt ggcacagggc ccatccccga gttcccggta
     3301 gatccttagg ctctccctca gatgggtacg cgccacttcc cgctcttccg aggagggtcc
     3361 cgcgttcctg tttaccagcg ctcccagttc gtagagggcc ttcgcccgta ccgccgtatt
     3421 tccattcccc gcggaaagcc ggagaaacgt ttcgagccag gatattcctt ctgtaaagta
     3481 gccgcgctga taccagaacc ttccgagcgt cgcggccagg tgcaaccctc gttctgcttt
     3541 cccctcgtgc gcgagccagg cgagggcgtc gcgcaggtcg tcgtgttcca tctccagtcg
     3601 ttcgagcccg accatctgtc gcggaccctc gacctcagcc ccttcggctt gtttgaggaa
     3661 ccaggtcgcg tgccggcggc gtgcgccggc cgctcttccc tccgcttcga gtttctgcct
     3721 tccgtattgc ctgaccggct ccagcatctt gtatcttacg tccccaggac cgtcgtccac
     3781 catcaccagc gaccggtcta ccagccgcaa gagaagctcg agtacgcaat cctccgaatc
     3841 gctagttcca cggccgaccg cctcggcact ctccagggtc caacttccag cgaacacgga
     3901 gagtcgcccg aacaacatct tttcctgttc gtcgaggagc tcgaagctcc agtccagcgc
     3961 cccttcgagg gtccgctgcc gcttcggggc cgtccggctg ccggtgagaa gcctcaagga
     4021 cccttcgagc ctcgtggaga tctgggcgac ggagagtgca cccacccggt ttgccgccag
     4081 ctctatggca agcggcaagc cgtccagttt ctggcaaacc tccgccacgg cctgcgcgtt
     4141 gcctggttcc agcgcgaagg agggaacacg caacgcggcc cgctcgacga aaagtcgtac
     4201 cgactctatc cccgcaaggt cctcgaccgc gaccggatgg cgcgtctccg gtatggtgag
     4261 cggcggaacc cgccagacca actctcctcc gacattgagc gtctcacgac tggtcgtaag
     4321 cacccggagg tcggggcagg agtcgagcaa cgactcgacc agacgggcca ccgcatctac
     4381 gaggtgctcg caattatcca gcaccagcag gaggtttttc gaagtcaggt actccttgag
     4441 cgccgcgaac aggggtcgac cgggttgttc gcgtaccccc aatgcctggg caaccgtctg
     4501 ggcgacgagt tctgggtcgc gccgcggggc aagctccacc agccagaccc cgtcctcata
     4561 agatccgacc aggctgttgg caacggccag cgcaagcctg gtcttgccaa gacccccagt
     4621 acccgtgagc gtcaacaaac gggtccccgc caacacccgc tcgacctctg ctcgctggtc
     4681 cgcgcgacca acgaagctcg tgcgctctgc cggcaggttg tgaggcccgg cacccgaagg
     4741 ggttgctggc atgacgtggg gccgggatcg gtctgccccg gatctatccg atgggtcccc
     4801 gggcagcatc tcctcgtaga gacgccggac cgcctcctgg agaccgggat cggtttgccg
     4861 cgaagcgacc tctcgtaggc gcacgtgctg gagcagggcc tcacgatgtc gaccgctcct
     4921 ggcgtacgct cgcatcagcg ctaccctggc ccactggtcc gatggcccag cctcgacagc
     4981 gacgttcaac gccgtgatag cgggatcgta ctcaccgcgt tcttcatgga tctcggccag
     5041 ctccactagc agcgagaggt acgtgttctt cagcgactcg cgccgattca tggtccactc
     5101 ttcgtagcga tcctccggca ggaggtctcc ggcgtagagg tttatggccg ccctgtaggc
     5161 ggcgggctcc cgagcgctcc tcctcgccga ggccgctgcc gcctcgaagg ccctcgcgtc
     5221 cacccagagc cgctcctccg gacacagaac gagcaggccg ccctggagct tcagataccg
     5281 gcccccttcg tccgccccga gaaccttacg cgcgacgtag agcgcctgcc tgagattgtt
     5341 ggcagcggcc ttcgggggcc gatccgacca cagatgttcc agaacctgct cgcgctgaag
     5401 ccgacatccc ggcgccaggg ccaggagctt gacaagcgcc gcagccttcc tcagcctcca
     5461 cgtatcctgc ccgacgaccc gccggcctac ccggatgcga aaaccgccga gtagctcgac
     5521 gcgcacctcc gaaccgccag ccgcgccgac gtacggtata tccgtcggta cgccgttcga
     5581 caagacttac caggttcccc ggctgcaaag cgcccaccat accctgcaag aggccatgct
     5641 atatagatag catacttgca ctccagcatc caggcattgt tcgtggggag gggttctcgt
     5701 gctacagaaa gtgccgcgca ccccgccttc gacgtggccg agatcggcga acctccggcc
     5761 gccgactccg gtccggctac cccggtgcac atgaggttac ccgcttagtg ctgctacctt
     5821 ccggtcctga cacggttcgc ggggccccat tgcccgggac caggccatca acgccggccg
     5881 caccggtttc taccgaaccc ggtctgcgcc tcaggcgggg atataggccc tgctaggcga
     5941 tcgcaggtac agggcatcgc cagctccccg cttagcgcgg ctcggagatt ctagcacgct
     6001 tcggcctttc ggcctacgct tgtcagcacg ctcgggctgg tgccctcgct tgtcagcacg
     6061 ttagggccct tcgggcctcc actttcagca cgctcccttc ggtcgctttc agccgatccc
     6121 gctgcggcct gcaccctgcc gctctaacga ttcgctgccc ggaagaggtt ggttgtcgaa
     6181 gcaaccgttg cgaaccgaga cgaccggccg aaggcgagag ccttcaggct cgaagcgttg
     6241 ccgacaagcg taggcgttag ccggagcgtg ctgactagcc ggagcgtgct cggagttgcc
     6301 tggcgagctc
//
LOCUS       DBNGVD010000386        12932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2644002, whole genome shotgun sequence.
ACCESSION   DBNGVD010000386 DBNGVD010000000
VERSION     DBNGVD010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2644002"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="AC16CM_10375"
     CDS             <1..883
                     /locus_tag="AC16CM_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140278.1"
                     /translation="VAAADGSVGRILDGHFNGVERVSLLAPEPLRSAELAAVGAGELR
                     LGVWGADPIPGEGVPARLVDGTEEKTITGVKTFCSGSTGLDRALVLVRGDAPGPPLLA
                     YVDLSSGVEIDPGWFKGAGMRASESHRVIFDGAPVLAVLGEPGELLREPYFSRDAVRT
                     AVSWAGIADAAVEATLDVLAAKAGGGDPDDVVSLAAGQILTAQGTIDRWLTHAAALAD
                     DDPASLPGISTQLRQAVASSCREILDEAARAVGSHPFATSGPLDRARRDLGLFLLQHR
                     LEPALLRRGRLAIAERS"
     gene            880..1455
                     /locus_tag="AC16CM_10380"
     CDS             880..1455
                     /locus_tag="AC16CM_10380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3140279.1"
                     /translation="MSGRLGREYFEKLYAESEDPWDFEVSQYEQNKYARTLSTLGDRT
                     FRRAFEAGASIGVFTEMLAGRCDELVAVDVSERAVSVARRRLAGKDHVRVERRTLPEQ
                     MPEGPFDLIVASEVLYYFPREEMLTVLSGFEHELAPGGVLLAVHWRRKTETYPLQGDE
                     VHELLVEHTRLLNKQTIVEPDYRLDLFEDEG"
     gene            1452..2624
                     /locus_tag="AC16CM_10385"
     CDS             1452..2624
                     /locus_tag="AC16CM_10385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3140280.1"
                     /translation="MNPEEHLVIVGGGPAGLATARAYRQTGGRARVTLLSSEPYAPYN
                     RPPLTKEYLRGEASPEDLPLESDGWYGDNDVELRLSVNVAAIRREDQTVELDDGEKLS
                     YDACVLATGSEPIRIPVPGAEDPEILVMRTIENSRRLQGRVGEDDTVVVVGSGFIGCE
                     AAASLAMRGAEVTQISLEESPQRERLGQEVGNRIEGWLRGYGVDLRLATNLEGIERSE
                     AGYELSMDGAGTLSAGTVVFGTGVAPRTGLAEEAGLEVDGGVVTDSSMRTSAPGIFAV
                     GDIARAYNESAGRHLSVEHWGDALEHGRIAGTVISGGEAAWGMVPGFWSTIGDETLKY
                     WAWGDGWDEQLFEDRGEAFTVWYGKEGTLVGVLSHNADEDYERGREPIERGASFPG"
     gene            2621..3451
                     /locus_tag="AC16CM_10390"
     CDS             2621..3451
                     /locus_tag="AC16CM_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3140281.1"
                     /translation="MKPALPHPALRACVVVPARNEQDLIASALRSLAAQTGIEPYEYE
                     VLVVLDNCTDATEKRARQVADDHPQLRLHLLDGPGLGSGHARRIGMDAACERLIGLGR
                     PKGLVASTDADTIVAPDWLSVQLDAVGRGARAIGGRIELDQGSLSEAAIRWHAERGKA
                     RHHKLLADPDRLGTTEHWQFSGASLALTAETYARVGGLEPRAALEDEGLEQVLREERI
                     PIDRLLSVRATTSSRLEGRASQGLAHDLAAASSRLDDTNTPHARGQDRLFPTVQGTRQ
                     "
     gene            complement(3542..3787)
                     /locus_tag="AC16CM_10395"
     CDS             complement(3542..3787)
                     /locus_tag="AC16CM_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140282.1"
                     /translation="MFFRRGHQDVHLRPTGSEDTFVVPAGVDTISVVATGAPGAVGFS
                     GGSAGRGARVAGTDVATDFDAAQGDSRIGIPQEGPKY"
     regulatory      complement(4010..4089)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4235..5509)
                     /locus_tag="AC16CM_10400"
     CDS             complement(4235..5509)
                     /locus_tag="AC16CM_10400"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR3140283.1"
                     /translation="MERRLEDRRLGRQDFSKPGGSGLASAALLGMAGRAIAATSHSGQ
                     GSPSGSSGMSISRQEWGSVDDEPVHLYTLSNGRMTVEITNYGSTVVSILVPDSAGRVD
                     NVALGFSSLEGYVENATNPADGGSGTTYFGAAIGRYGNRIANGRFRLNGKTCELPVNN
                     GPNTLHGGPGAFNMRVWGAAPEEGPNSVALELACTDPGGHNGFPGTVETEVTYTLSRD
                     DELRIDYRATTDQTTVINLTNHTYFNLAGEGSGNVYGHLLQINAGSYTPTDETQIPTG
                     EISSVEGTPFDFRTAKPIGRDIREKDEQLVRAHGYDHNWVLDGSGMRLISSVEDPQSG
                     RVLLTYTDQPGVQFYSGNFLVGELVGKGGNTYRQGDGFALETQHFPNSPNQPDFPSTV
                     LNPGDEFTSTTIYGFSTAGSPDLQAARTAHIS"
     gene            complement(5617..6414)
                     /gene="iolB"
                     /locus_tag="AC16CM_10405"
     CDS             complement(5617..6414)
                     /gene="iolB"
                     /locus_tag="AC16CM_10405"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR3140284.1"
                     /translation="MSDLVVGIVQNPAEDGSVVKVTPESAGWEYVGFEVLRLDAGRTV
                     ERETGDEEVCLVLLEGYCNVSAGDEAWTGIGGRDSVFEGPPYAVYLPPGTAYSVEADS
                     YLELAVCSAPAENGVEAFLIRPEDIEVEMRGAGNAERQVHPILMAEGQAERLLVVEVI
                     TPNGHWSSYPPHKHDQDDLPEESYLEETYFHKVQPPQGFAVQRVYTGDRSLDETMTVV
                     DGDTVLVPRGYHAVSAPPGYDLYYLNVMAGPVREWKFRNDPDHEWLF"
     gene            complement(6472..8331)
                     /gene="iolD"
                     /locus_tag="AC16CM_10410"
     CDS             complement(6472..8331)
                     /gene="iolD"
                     /locus_tag="AC16CM_10410"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     carbon-carbon bonds, in ketonic substances [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HIR3140285.1"
                     /translation="MAQALVRFLANQYVERDGVEQRFFAGCFGIFGHGNVAGMGQALQ
                     QYPEDMPYYQARNEQAMVHVASAYARQKNRLQTLACTSSIGPGATNLVTGAALATINR
                     LPVLLLPGDIFASRGPDPVLQQLEAPHDGTLSVNDSLRPVSRYFDRIMRPEQIMPAAL
                     SAMRVLTNQAETGAVTLALPQDVQTEARDYPEEFFDKRVWRVGRPLPDADFLEDAVSL
                     IRASVRPMIVSGGGTIYSEATGALHRFAESTGIPVGETQAGKGSMPYDHPLALGAVGA
                     TGTFAANRAAREADLVIGVGTRWSDFTTASKTAFQNPSARFVNVNIADFDAHKHAGLA
                     LVGDARATLTALDSRLVGDQVGPEYRDQSRRANADWDAEVERLYSLGHAPLPAQSEVI
                     GAVNQASRPRDVVVCAAGSMPGDLHKLWRTRDAKGYHVEYGYSTMGYEIAGGLGIKMA
                     DPDREVYVMCGDGSYLMMNSEIVTSIQEGYKLIIVLVDNSGFSSIGALSRSVGSQGFG
                     THYRYRKNGSIGLDSEDDPGGVLPVDLATNAESLGALVMRTTAVAELRDALEGARQAD
                     RTVVIHIPVDRYEGVPDYESFWDVPVAEVSEMETVVAAREEYVQNRRAERRHL"
     gene            complement(8566..9456)
                     /locus_tag="AC16CM_10415"
     CDS             complement(8566..9456)
                     /locus_tag="AC16CM_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3140286.1"
                     /translation="MSARVAGAPISWGVIEVPDWGYQMPADRVLREAASLGLTAMEAG
                     PEGFLPDGTTEVAAVLGGHGLSLVGGFVPAVLHVPDVLDEQLAFVERRADFFAAAGAD
                     VVVLAASTGSENYGEVFEADEDAWSTLFASLGEVERICERHGVAVSLHQHFGTVIERD
                     DQLRRFLDGSDMGLCLDTGHLAIGGSDPVEIARLAAGRVNHVHLKDVDYELAARLASR
                     ELDFREAAEQGAFRPLGDGDIDIGRLLEVLDDGGYSGWYVLEQDSVVEDEPPEGEGPL
                     RDVKKSLTFVENSLNGGSKG"
     gene            complement(9453..10496)
                     /locus_tag="AC16CM_10420"
     CDS             complement(9453..10496)
                     /locus_tag="AC16CM_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3140287.1"
                     /translation="MQDPFQVAVGVIGTGGMGTMHAENVHDVIANARLVAVADLDTER
                     AGSLAERCGSAAVYEDPVALIRDDAVEAVVIASPDPTHLPLVLECLENEKPVLCEKPL
                     AEDASGALEILEAEGRLGRKLVQVGFMRRYDPEHVAVKEAADSGDIGVPMLFKGWHRN
                     LDQEPGLDSASVVTNSTIHDLDSARWYLGEEIEEVYALGTNTAPKLGRDVLDLQVIQF
                     RTTGGRLGTIETYVVSEYGYEVGVELVGKSGTAQLGPSNGAVVRRGLAISQEVEEDWL
                     QRFREAYVIELRSWVESLRGNAPAGPDAWDGYAALVVADACIASLRSRSPQKVPALEA
                     PALYANVGSEVVG"
     gene            complement(10556..12496)
                     /locus_tag="AC16CM_10425"
     CDS             complement(10556..12496)
                     /locus_tag="AC16CM_10425"
                     /EC_number="2.7.1.92"
                     /EC_number="4.1.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF047869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     5-dehydro-2-deoxygluconokinase/5-dehydro-2-
                     deoxyphosphogluconate aldolase"
                     /protein_id="HIR3140288.1"
                     /translation="MAGTMTGGRERVVELVCIGRTCVDLYAEQEGARLEDVQSFRKYV
                     GGSAANIALNTARLGVRTAMLTRVGEEQMGRFVRRTMADTGVDVSHVTFDPEHLTPYV
                     LLAIRDVDDFPRIFAYGDAADMALEEEDVDPDFIASSEALLVTGTPLSRPGSRSATFK
                     AVRAAGEAETRVVFDLDYRPVFWGVASHQQGGEMFVESAEVTEQYRSVLPSCDLVVGT
                     EEEIRIAGGHSDTRDSLLAIRSVCSATIVLKVGAMGAIVFPGEIPEDLQDGVSVPGFP
                     VEVFNSVGAGDAFMSGFLSRWLRDEPLEECLRIGNACGAIVVSRHGCSPAMPTPEELR
                     YFLDLTDRPRRLRDDGWLEHLHRVTTRREPQELCVLAIDHRWQLEDAVEEIGVDRGRL
                     RELKLLLARAFMRVAEDEPSAGVLLDDIYGRKALEEITGSGVWISHAVEVAGSRPVDF
                     VGGPNPAATIRTWPEEHVVKCNLYMHPDDDPGISEVQERSVRQLFDACVKNDRRLLLE
                     VQAPPSTSYGENDLVQLIERFYEIGVRPDWWKLPPDRDPEVWRRIGEVVSEGDPYCAG
                     VLVLGQALEEEELARFFAAAAAEPRCNGFAIGRSIYGAEARLWLAGETSEEDLMSSVT
                     ERYGRMISLWRGRNEQQNRVTR"
     gene            complement(12816..>12932)
                     /locus_tag="AC16CM_10430"
     CDS             complement(12816..>12932)
                     /locus_tag="AC16CM_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140289.1"
                     /translation="AIVKEAHETFWGGYSGYFEDPDRHLWEVVWNPMLLTGD"
BASE COUNT         2214 a         4434 c         4047 g         2237 t
ORIGIN      
        1 cgtagccgcc gccgacggct cggtgggccg cattctggac ggtcatttca acggcgtgga
       61 acgggtctca cttctcgccc ccgaacccct gcgttcagcc gagctcgcgg ccgtgggtgc
      121 gggcgaactg cgcctcgggg tctggggagc ggatcccatc cccggtgagg gagtccctgc
      181 ccgcctcgta gatggcacgg aggaaaagac gatcaccggc gtcaagacct tctgttccgg
      241 ttcgaccggc cttgaccggg cgctggttct ggtgcgcggc gatgcccctg ggccacccct
      301 tctcgcctac gtggatctct cgagcggcgt ggagattgat ccgggctggt tcaagggcgc
      361 cggcatgcgc gcttcagaga gccaccgcgt gatcttcgac ggggcgcccg tgctcgcggt
      421 tctcggcgaa cccggcgaac tccttcgcga gccctacttc agccgcgacg ccgtgcgcac
      481 ggccgtgagc tgggcgggga tcgccgatgc ggccgtcgag gcgacgctcg acgtgctggc
      541 ggcgaaggct gggggtggag atcccgacga cgtggtctcc ctcgcagccg gacagatact
      601 cacagcccag ggaaccatcg accgctggct gacgcacgcg gccgccctcg cggacgacga
      661 tcccgcctcg ctacccggca tctcgaccca gttgcggcag gccgtcgcca gttcttgccg
      721 cgagatactg gacgaggcgg cgagggcggt cgggtcgcat ccgttcgcca cctccggccc
      781 gctggaccgg gcgagacgag acctcgggct gttcctgctt cagcaccgcc tggaacccgc
      841 gctcctgcga cgcggccggc tggccatcgc ggagaggtct tgagcggccg actggggcgc
      901 gagtacttcg agaagctcta cgccgagtca gaagacccgt gggacttcga ggttagccag
      961 tacgagcaga acaagtacgc cagaaccctg tcgacgcttg gagaccgtac cttccggagg
     1021 gccttcgaag cgggggcttc catcggggtc ttcacggaga tgctggcggg ccgttgcgac
     1081 gagctggtcg ccgtggacgt gtcggagcgg gcggtctcgg tggcccggcg acgcctcgcc
     1141 ggaaaggacc acgtcagggt cgagcggagg accctaccag agcagatgcc agaggggccg
     1201 ttcgacctga tcgttgcctc cgaggttttg tactactttc caagggaaga aatgctaacg
     1261 gtactgagcg gcttcgagca tgagctggca ccggggggag ttctgctcgc cgtccactgg
     1321 cgcaggaaaa cagagacata cccattgcag ggagacgagg tgcacgaact tctcgtggag
     1381 catacgcggc ttctaaacaa gcagaccatc gtcgagccgg attaccgcct cgacctcttc
     1441 gaggacgagg ggtgaacccc gaagaacacc ttgtgatcgt gggcggcggt cctgccggcc
     1501 tcgcgacagc ccgggcctac aggcagaccg gaggacgggc gcgcgtcacg ctcctgtctt
     1561 ccgagccgta cgctccatac aatcgtccgc ccctgaccaa ggaatacctg aggggcgaag
     1621 cctcccccga agacctgccc ctcgaaagcg acggctggta cggggacaac gacgtggagc
     1681 tcaggctctc cgtgaacgtc gcagcgattc gaagggaaga ccagaccgtc gagctggacg
     1741 acggcgagaa gctatcttac gacgcttgcg tgctcgccac gggctccgag ccgatccgga
     1801 tccccgttcc cggtgcagaa gacccggaga tactcgtgat gaggaccatc gagaactcga
     1861 ggcgcctgca gggccgcgtt ggagaagacg acaccgtcgt ggtcgtgggg agcggcttca
     1921 tcgggtgcga ggccgccgcc tcgctcgcga tgcggggtgc tgaggtgacc cagatcagcc
     1981 tggaagaatc gccgcaacgc gaacgtctcg gacaggaggt cgggaaccgc atcgaaggct
     2041 ggctccgcgg ctacggcgtc gacctgcgcc tggccacgaa cctggagggc atcgagcgct
     2101 cggaagccgg ttacgagttg tccatggacg gagctggcac tttatccgcc ggcaccgtgg
     2161 tcttcggcac tggcgtcgcg ccccgcaccg gtctcgctga ggaggccggc ctggaggtag
     2221 acggcggggt cgtaacggac tcttcgatgc gaaccagcgc ccccggcatc ttcgcggttg
     2281 gcgacatcgc cagagcctac aacgagtctg ccggccgtca ccttagcgtc gagcactggg
     2341 gggatgcgct cgaacacgga cgtatcgcgg ggaccgtgat ctccggcggc gaagccgcat
     2401 ggggtatggt gccaggattc tggtccacca tcggcgacga gaccctgaag tactgggcct
     2461 ggggagacgg ctgggacgag cagctcttcg aggacagggg cgaagccttc accgtctggt
     2521 acgggaaaga aggaacgctc gtcggcgttc tctcccacaa cgcggatgag gactacgaac
     2581 gcggacgcga gcccatcgaa cgcggggcgt ccttccccgg atgaagcccg cgctgccaca
     2641 cccggccctc agggcctgcg tcgtcgtgcc cgcccgcaac gagcaggatc tcatcgcctc
     2701 cgcgctgcgg tccctggcgg cccagaccgg catcgaacct tacgagtacg aggtgttggt
     2761 cgtgctcgat aattgcaccg acgcaaccga gaaacgggcg aggcaggttg ccgacgatca
     2821 tccccaactc cggctccatc tgctcgacgg gccgggtctt gggtcgggcc acgcgcgaag
     2881 gatcggcatg gacgccgcct gcgagaggct gatcggcctg gggcgcccga aggggctcgt
     2941 ggcatccaca gacgcggaca ccatcgtcgc ccccgactgg ctctcggtac agttagatgc
     3001 cgttgggcga ggcgctcgcg ccatcggggg ccgaatcgag ctcgaccagg gatcgctatc
     3061 cgaggctgcc attcgctggc atgccgagcg agggaaggcc cggcaccaca agcttctggc
     3121 cgatccggat aggctcggca cgacggagca ctggcaattc agcggcgcct ctcttgctct
     3181 gacggccgag acctacgcca gggttggcgg cctggagccg agggcggcgc tggaagacga
     3241 aggcctggaa caggtccttc gcgaggaacg tatcccaata gaccgcctgc tctccgtccg
     3301 ggccaccact tcatcgcggc tggagggtcg cgccagccag ggcctcgccc acgacctcgc
     3361 cgccgccagt tcccgcctcg acgatacgaa cactccacat gctcgtggac aggatcgatt
     3421 gtttcccaca gtgcagggca cccgtcagta ggtcctctac cctcttcgga caccctccca
     3481 cagagtcaaa tacagagggg ctgaatcgtt acctaccaga ttcggcccct ctggttgcct
     3541 gctagtactt cggtccctcc tgggggatgc cgatcctgga atctccctgg gctgcgtcga
     3601 agtcggtcgc cacgtcggta ccggccaccc gagcaccgcg accggcagag ccaccactaa
     3661 atcccacggc gcctggagcg ccggtggcca ccacgctgat ggtgtcaaca ccggccggca
     3721 ccacgaaggt gtcctccgag ccagtcgggc gaaggtgcac gtcctgatgc ccgcggcgga
     3781 agaacaacta ccccccgccg cgcgggctgg gctcgcgttg taggcgagca ggaccgccac
     3841 tagcaacgtc acgaacgcga ccgacgcggc cacgcgcaag ccgggcccga cacgtcttct
     3901 cacgccatgg cccagtatcg ttaacgcatg cacaccgatt ccccttccgt ccgtacgggc
     3961 gggacgtggt gctcgggccc acgcgctaga gagactccat atctcaccgt tcggcaaccc
     4021 ggctacctgc ctcgtcgcag gcccgtggct ttgcgccccc gcctcacggc gggtttgcct
     4081 ttgtcgcggc actggagtgc gcggaagccc cgagcccagc acgccgcagc cgaggtcggc
     4141 atccaccaaa tgggctagat ccggggcgca attcccgggg cgggcacgcg caatacttac
     4201 gcactacccg ccccgaacta cacctcgccg cccattaact gatatgagcc gtccgcgcgg
     4261 cctgcaggtc ggggcttccc gccgtcgaga aaccatagat cgtcgtcgag gtaaactcgt
     4321 cccccgggtt caggaccgtg gagggaaaat cgggttggtt gggcgagttc gggaagtgct
     4381 gggtctccag cgcaaaaccg tcgccctgcc ggtaggtgtt gccacccttt ccaacaagtt
     4441 cgccgaccag aaagttgccc gagtaaaact gcacgccggg ctggtccgtg taggtcagca
     4501 ggacgcgacc gctctgcgga tcctcgacac tagagatcag gcgcatcccc gaaccatcga
     4561 gcacccagtt gtggtcgtaa ccgtgcgccc gcaccagttg ctcgtctttc tcgcggatgt
     4621 cgcggccgat tggtttcgcg gtgcggaagt cgaagggggt gccctcgacg gacgagatct
     4681 cgcccgtcgg gatctgggtc tcgtcggtcg gcgtgtagga cccggcattg atctggagca
     4741 ggtgcccgta cacgttcccg gacccttcgc cagcgaggtt gaagtaggtg tggttggtca
     4801 ggttgatgac ggtcgtctga tcggtggtcg cacggtagtc gatgcgtagc tcgtcgtccc
     4861 gcgagagcgt atacgtcacc tcggtctcga cggtccctgg aaagccgttg tggccgccgg
     4921 ggtcggtaca ggcgagctcg agcgccacgg agttcgggcc ctcctccggg gcggcgcccc
     4981 agacccgcat gttgaacgca ccagggccgc cgtgcagcgt gttggggccg ttgttcacgg
     5041 ggagctcaca ggtcttgccg ttcaggcgga acctgccgtt ggcgatccgg tttccgtagc
     5101 gaccgatggc ggccccgaag tacgtggtgc ccgagcctcc atcggcgggg ttcgtggcgt
     5161 tctccacgta gccttccagg cttgagaaac cgagggcgac gttgtctacc cgtcccgcgc
     5221 tgtccggcac gaggatggag acgacggtcg agccgtagtt ggtgatctcc acggtcatcc
     5281 gcccgttgga gagcgtgtag agatgaacag gctcgtcgtc cacgctcccc cattcttgcc
     5341 tggagatgct catgccgctg ctccccgaag gtgacccctg gcccgaatgc gaagtagccg
     5401 cgatggcccg ccccgccatg ccgagaagcg ccgcgctggc aagcccggaa ccgcccggct
     5461 tcgagaaatc ctgtcgaccg agtctacgat cctcgagtcg tcgctccatc ctggcacgct
     5521 ctcctttaca ccattcgtag gctacaggtt tcagtaaaac cacctgttgt ctgtagcctt
     5581 gttgccaatt ctggttctct tgcttcaagc atccgcctag aatagccact cgtggtcggg
     5641 atcgttcctg aacttccact cgcggacggg ccccgccatg acgttgaggt aatacaggtc
     5701 gtagccgggg ggcgcggaga ccgcgtggta gccgcgtggg acgagcacgg tgtcaccgtc
     5761 taccacggtc atggtctcgt ccagggagcg gtcgccggtg tagaccctct gcacggcgaa
     5821 accctgggga ggttggacct tgtgaaagta ggtctcctcc agatagctct cctcaggcag
     5881 gtcgtcctgg tcgtgcttgt gcggcgggta gctcgaccag tgcccgttgg gagtaatgac
     5941 ctctacgacg agcagcctct ccgcctgtcc ttcggccatc aagatagggt gaacctgacg
     6001 ttcggcgttg ccggcccctc gcatctctac ttcgatgtct tcagggcgga tgaggaacgc
     6061 ctcgacaccg ttctcagccg gggccgagca gactgccagc tccaggtagg aatcggcctc
     6121 cacagagtat gccgttcctg gcggaagata gaccgcgtaa ggtggcccct cgaacaccga
     6181 gtcgcgtccc ccgatgcccg tccacgcttc gtccccggcc gagacgttgc agtagccttc
     6241 gagcaggacc aaacagactt cctcgtctcc cgtctctcgc tccacggtcc ttcccgcatc
     6301 gaggcgcagc acctcgaagc ccacgtactc ccacccggcc gactccggcg tgacctttac
     6361 cacgctgccg tcttccgctg gattctggac tatgcccact acaagatccg acacgttcca
     6421 atccccttcc cagacctggt gatccctggc tacagacgtc cctgaatacg ctcagaggtg
     6481 acgtcgctct gcacgcctgt tctggacgta ctcctccctg gcggcgacga cggtctccat
     6541 ctccgacacc tcggcgaccg gcacgtccca gaacgattcg tagtcaggca caccctcgta
     6601 gcggtccaca gggatgtgga tcaccacggt ccggtcagcc tgcctggcac cttctagcgc
     6661 gtcccggagt tcggcgaccg cggtggttct catcaccagc gcgccaaggc tctcggcgtt
     6721 cgtggccagg tccaccggca gcaccccacc cgggtcatcc tcggagtcga ggccgatgga
     6781 gccgttcttg cggtaacggt agtgggtgcc gaagccctga gatccgacag agcgcgacag
     6841 ggcgccgatt gaggagaaac cggagttgtc cacgagcacg atgatgagct tgtagccctc
     6901 ctggatggag gtgacgatct cggagttcat catcaggtag gacccgtcgc cgcacatcac
     6961 gtacacctca cggtcggggt ccgccatctt gatccccagg ccgccagcga tctcgtatcc
     7021 catggtcgag tatccgtact cgacgtgata gcccttggcg tcgcgggtcc tccagagctt
     7081 gtgaaggtcc ccgggcatgg aaccggcggc gcacaccacc acgtcccgcg gacgcgaagc
     7141 ctggttgacc gccccgatga cctcgctctg cgccgggagc ggcgcgtgcc ccagcgaata
     7201 cagacgttct acctccgcat cccagtccgc gttggcacgc cggctctgat cgcggtactc
     7261 cggccccacc tggtctccaa caagacggct atccagggcc gtcagcgtgg cgcgggcgtc
     7321 tccaacgagg gcgagcccgg cgtgcttgtg ggcgtcgaag tcggcgatgt tgacgttgac
     7381 gaagcgggcg ctagggttct ggaaggcggt cttcgaggcc gtagtaaagt cgctccacct
     7441 cgttccaacc ccgatgacca ggtcggcctc gcgggccgcc cggttggcgg cgaacgtacc
     7501 cgtggcgccc acggccccta gcgccagagg gtgatcgtaa ggcatcgaac ccttgccggc
     7561 ctgcgtctcg ccgacgggga ttccggtact ttcggcaaag cggtgcaggg cccccgtcgc
     7621 ctcggagtag atggtgcccc cgccgctgac gatcatcggc cgtactgacg cccgtatcaa
     7681 gctcacggcg tcctccagga agtctgcgtc aggaagcggc cggccaacac gccagacgcg
     7741 cttgtcgaag aactcctctg gatagtcgcg ggcctctgtt tgcacgtcct gcggaagggc
     7801 gagggtcacg gccccggtct cggcctggtt cgtgagcacg cgcatggcag acagggcggc
     7861 cggcatgatc tgctcgggac gcatgatcct gtcgaagtag cgtgacacgg gccgcaagct
     7921 atcgttgacg gagagggttc catcgtgtgg ggcctccagc tgctgcagta cggggtcggg
     7981 cccgcgcgag gcgaagatgt cccccggaag gagcaggacg ggcaggcggt ttatggtggc
     8041 cagcgccgcc cccgtcacca ggttggtcgc gccagggcct atagaagacg tgcaggcgag
     8101 agtctggagc cggttcttct gtcgcgcgta ggcagatgcg acgtggacca tggcctgctc
     8161 gttgcgtgcc tggtagtagg gcatgtcctc ggggtactgc tggagggcct ggcccatgcc
     8221 cgcgacgttg ccgtgtccga agataccgaa gcagcccgcg aagaagcgtt gctcaacccc
     8281 gtcccgttcc acatactggt tcgcgaggaa ccgaaccagc gcctgggcca tcgtgagtcg
     8341 taccgtgccc aactccctca cctccggttc gccgtgcgta caagggtcat gctacaaggg
     8401 acagcctact ccacctcggt tctggccgcc cgacctttcg gttagacttc ctgcattcgg
     8461 ccgcccactt atgcgattct cgactttaac ggtatcgtta accaccgcag gtcatagcca
     8521 ctagcagcgt gacgaccagc aaccacagac gatttcacgt ccggctcacc ctttcgagcc
     8581 accgttcagg ctgttctcca cgaacgtcag gctcttcttt acatctcgta gcgggccttc
     8641 gccctccggc ggttcgtctt ccaccaccga atcctgctcc agcacgtacc agcccgagta
     8701 ccctccgtca tccaggacct ccagcagccg gccgatgtcg atgtcgccgt cgccgagggg
     8761 ccggaacgct ccctgctccg cggcctccct gaaatcgagc tccctgctcg ccagccgggc
     8821 agccaactcg taatccacgt ccttgaggtg tacgtggttg accctgccgg ccgccagcct
     8881 cgctatctca acgggatcgc tgcccccgat cgcgaggtgg ccggtgtcca ggcagagccc
     8941 catgtcggag ccgtccagga agcgcctgag ctggtcgtcg cgctcgatga cggtcccgaa
     9001 gtgctggtgc agggagaccg ctacgccatg acgctcgcag atccgctcga cctccccgag
     9061 cgacgcgaac aacgtgctcc acgcgtcctc atccgcctcg aatacctccc cgtagttttc
     9121 cgaccccgtt gaggccgcga gcacaaccac gtcggccccc gccgcggcga agaagtccgc
     9181 ccttctctcg acgaaggcaa gttgttcgtc aagcacgtcc ggcacgtgca acacggccgg
     9241 cacgaagccg ccgaccaggc tcagaccgtg tcctcccagc accgcagcga cctcggtggt
     9301 cccatcaggc agaaacccct ccgggccggc ttccatcgcc gtcaggccga gcgacgccgc
     9361 ctcgcgcagc accctatcgg ccggcatctg gtagccccaa tccggaacct cgataacacc
     9421 ccaggagatg ggggcgccgg cgacgcgcgc gctcacccga ccacctccga acccacgttt
     9481 gcgtaaaggg ccggggcctc tagcgccgga accttctggg gagatctgga gcgtagcgag
     9541 gcgatgcagg catctgcgac cacgagcgcg gcgtagccgt cccaggcgtc cggtccggcg
     9601 ggagcattgc cccgcaatga ctcgacccaa ctccgcaact ctataacgta agcttcgcgg
     9661 aacctctgga gccagtcttc ctcgacctcc tgggagatcg caaggccccg gcgaaccaca
     9721 gcgccgttcg atggtcccag ctgagcggtg cccgacttgc caaccagctc cacgcccacc
     9781 tcgtagccgt actcgctgac cacgtaggtc tcgatcgtgc caaggcgccc gcccgtagtg
     9841 cggaactgga tcacctgcag gtcgagcacg tctcggccaa gtttcggggc cgtgttcgtc
     9901 cctagggcgt agacctcctc gatctcctcc ccgagatacc accgggcgga gtcgagatca
     9961 tggatggtcg agttcgtaac gacagaggcg gaatccaggc ccggctcctg gtccaggttt
    10021 ctgtgccaac ctttgaacag catgggcacg ccaatgtccc ccgagtcagc cgcctccttg
    10081 accgcgacgt gctccggatc gtaccgccgc atgaagccca cctgaaccag ttttctgccc
    10141 agccgcccct cggcctccag gatctcgagg gccccgctgg cgtcttcggc gagcggtttc
    10201 tcgcacagca ccggcttctc gttctccagg cactcgagca ccagcggcag gtgggtcggg
    10261 tccggcgagg cgatcacgac cgcctcaacg gcatcgtcac ggatcagcgc tacgggatcc
    10321 tcgtagacgg ccgcggaacc acacctttcg gcgaggctgc cggcccgctc ggtgtcgagg
    10381 tcggcgaccg cgaccagcct ggcattagcg atcacgtcgt ggacgttctc cgcgtgcatg
    10441 gtgcccatgc cgccggttcc tatcaccccg accgccacct gaaaagggtc ttgcacctga
    10501 gcctcctcac gttcgttgct gattggtctc gtccgaccgc ctctgcggga accgctcatc
    10561 gggttacccg gttctgttgc tcgttcctgc cccgccagag cgagatcatg cgcccgtacc
    10621 gctccgtgac cgaggacatc aggtcctcct ctgaggtctc gccggccaac cagagccgcg
    10681 cctctgcgcc gtatatagac cgacctatcg cgaacccgtt gcaacgcggc tcggcggccg
    10741 cggcggcgaa gaaccgggcg agctcctctt cttcgagggc ctggccgagc accagtacgc
    10801 cggcgcagta ggggtcgccc tcgctaacga cctcgccgat cctcctccac acctcggggt
    10861 cccggtctgg cgggagcttc caccagtcgg ggcgaacccc gatctcgtag aaacgttcga
    10921 ttagctggac gaggtcgttc tcgccgtagc tggtggaagg gggagcctgg acctccagta
    10981 gtagccgccg gtcgttcttg acgcacgcgt caaagagttg ccggacggat ctctcctgta
    11041 cctcgcttat cccgggatcg tcgtccgggt gcatgtagag gttgcacttg acgacgtgct
    11101 cctcaggcca ggtcctgatg gtggcggcag gattcggccc gccgacaaag tctacgggac
    11161 ggctcccggc gacctcgacg gcgtgcgaga tccagacccc gctaccggtg atctcctcca
    11221 gggcctttcg cccgtagata tcgtccagga ggacgccggc cgacggctcg tcctcggcaa
    11281 cccgcatgaa cgcccgcgca agaagcagct tcagctcccg caatcgcccg cggtccaccc
    11341 cgatctcctc tacagcgtct tctagttgcc agcggtggtc gatcgcgagc acgcacaact
    11401 cttggggctc ccggcgtgtg gtgacccgat gcagatgctc cagccacccg tcgtcacgca
    11461 gccggcgcgg cctgtcggtc aggtccagga agtaccgcag ttcctccggg gtgggcatgg
    11521 ccggcgagca gccgtggcgg gagacgacta tggcaccaca ggcgttcccg atccgcaggc
    11581 actcctccag cggctcatcc cgcagccacc tggatagaaa accgctcatg aaggcgtcgc
    11641 cggcgccaac cgaattgaag acctccacgg gaaagccggg tacgctgacg ccatcctgca
    11701 ggtcctcggg gatctccccg gggaaaacaa tggcccccat cgcaccaacc ttgagcacga
    11761 tcgtggcgct gcagaccgag cggatggcca gaagcgagtc ccgggtgtca gaatggccgc
    11821 cggctatccg gatctcctct tccgtaccca cgacgaggtc gcagctaggt agcacggacc
    11881 tgtactgttc cgtgacctcc gcgctctcga cgaacatctc tccaccctgc tggtgggaag
    11941 ccacgcccca gaacacaggc ctatagtcaa ggtcgaaaac taccctggtc tccgcctccc
    12001 cggctgccct tacagccttg aacgtcgccg accggctacc tggacgcgag agcggagtac
    12061 cggtaacgag cagcgcctcc gatgaggcta tgaagtcagg gtccacgtct tcttcctcga
    12121 gcgccatgtc ggcggcgtcg ccgtaggcaa agatccgggg aaaatcgtcc acgtccctta
    12181 tggcgagcag cacatagggc gtcaggtgct ccggatcgaa ggtaacgtgg ctcacgtcga
    12241 cgcccgtatc cgccatggtc cttcgcacga agcggcccat ctgctcttcc ccgactcgcg
    12301 tgagcatggc ggtcctgacg cccaaccggg ccgtgttcag ggcgatgttg gccgcgctgc
    12361 caccgacgta cttgcggaag gactgcacgt cctcgagtct ggctccctcc tgctccgcgt
    12421 agaggtccac gcacgtacgt ccgatgcaca cgagctccac cacccgctcc cgaccaccgg
    12481 tcatggtccc ggccaaccca cgccgatatc ttctgccacg cttcccctcc ccctctagcc
    12541 cccactacga cgctggctga caggccgtca gggttccaga cttcccaacg ccgcacccgt
    12601 cagcgccctg gacccttccc cgactctccc cataccagac ggagccgtgc gaatggcccg
    12661 acatcagaat atcagcaaca accgaacttc tcaggcacgc caccggacga actgtccacg
    12721 cgtacaagtt gcaggcgtgg gcagtccgct gacgggtgct acatgtcgaa gttacagggc
    12781 cccggcgagg gctcagattt gagtgtggtc gcgcctcagt ctccggtcag cagcatgggg
    12841 ttccacacca cctcccagag gtgtcggtcc gggtcttcga agtagccaga gtagccgccc
    12901 cagaaggtct catgggcttc cttcacgata gc
//
LOCUS       DBNGVD010000387         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1913642, whole genome shotgun sequence.
ACCESSION   DBNGVD010000387 DBNGVD010000000
VERSION     DBNGVD010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1913642"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16CM_10435"
     CDS             <1..187
                     /locus_tag="AC16CM_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140290.1"
                     /translation="PRVFERFHRVDPSRTDGGVGLGLSIARQIAEAHAGEIRVSSTPG
                     EGSTFTLLLPKQLRPRT"
     gene            316..675
                     /locus_tag="AC16CM_10440"
     CDS             316..675
                     /locus_tag="AC16CM_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3140291.1"
                     /translation="MMNRKKLVVGTVAVAVLTLGAGTAVIAQQGSLMGREDETPGHDD
                     AATEQPITGPSLERASQAALAHVDGGKVTETETGDEEGFYEVEITRADGGQIDVHLNR
                     NFDVIGTEGENATEDGS"
     gene            815..1648
                     /locus_tag="AC16CM_10445"
     CDS             815..1648
                     /locus_tag="AC16CM_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140292.1"
                     /translation="MVLLVIITAVSLAAAGCGERDRESADVRTSSASWEGGALPQGSE
                     PVDLDPENFTTKIDHPYWPMVPGTRWTYRETDQEGAELRVVVTVTKQTKKIANGITAR
                     VVRDTVTEDGEIVEDTFDWYAQDERGNVWYMGENTAEFEDGELATKSGSFEAGVDGAQ
                     PGIIMPANPKDGMQYRQEYYKDKAEDNGEILSTDEMAEVPYGQFDNALLTKDTITIEP
                     DVLEYKLYAPRVGPVLAVGVSGGPGSREELVELDDASEEWTSKSGTAPLGEGPIAPAK
                     P"
     gene            complement(1736..1957)
                     /locus_tag="AC16CM_10450"
     CDS             complement(1736..1957)
                     /locus_tag="AC16CM_10450"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140293.1"
                     /translation="MAGSMDVGVGGANVAQQCIGAGLIDEVGIDLVSVLLGEGVSSFD
                     RLRTGPIELERTMVVEAPGVTHLRFRVVK"
     gene            complement(2027..2326)
                     /locus_tag="AC16CM_10455"
     CDS             complement(2027..2326)
                     /locus_tag="AC16CM_10455"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140294.1"
                     /translation="MGKVATGFSTSLDGFIAHPYGDVGPLFDWYFAGDTEYTVPGGNF
                     TLRVSAQSAELLSESYGRMGELVVGRRHFDHAGAWGGRHPMDVPVFVVTHAVPRE"
     gene            complement(2393..>2664)
                     /locus_tag="AC16CM_10460"
     CDS             complement(2393..>2664)
                     /locus_tag="AC16CM_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140295.1"
                     /translation="DHSGEGGFRVVQLTPPGSACSVAVGSGIVHTPPGSVRGLHLVVA
                     DIEAARAELSERGVEVGEVQDLGGILYAGFSDPDENGWTLQQMPY"
BASE COUNT          526 a          906 c          817 g          415 t
ORIGIN      
        1 gcctcgggtc ttcgagcgtt tccatcgggt agacccctcc cgcaccgatg gcggcgtggg
       61 cctcgggctc tccatagccc gccagatcgc cgaggcccac gccggcgaga taagggtgag
      121 tagcacgccc ggcgaaggct cgacgttcac gctgctcctc ccgaagcagc ttcgaccccg
      181 tacctgagca ccccgagcgt ctccttcgtt ccctaactct ccccttccag gcaaactcca
      241 atgctgttct cagccccctc taaggtttcg gtgtgatact ccctcgtgca agcccgcaac
      301 gataaaggag gccagatgat gaacagaaag aagctcgtcg tcggcacggt agcggtagcc
      361 gttctaaccc tgggcgccgg cacggctgtc attgcccagc agggctcgct gatgggacgg
      421 gaggacgaga cgccgggtca cgacgacgcc gccaccgagc agccgatcac cggcccgtcg
      481 ttggagaggg ccagccaagc cgccctggcc cacgtggacg gcggcaaggt aaccgagacc
      541 gagaccggcg acgaagaagg cttctacgag gtcgagatca cccgcgcaga tggcggccaa
      601 atcgacgtgc atctgaaccg caacttcgac gtcattggca ccgagggcga gaacgcaacc
      661 gaggacggct cctaggcgcc taccgtgacg ggacgacccg ccacgaagcg ccgcctgtca
      721 gcaagactat caagctgagt gctgatggcg cgaactgcgg aaatcttgac cctctgtaat
      781 tcgcgccgag atcgggaggc agttggtgta caggatggtt ctactcgtga tcatcacggc
      841 ggtctcgctc gccgcagcag ggtgcggcga gcgggaccgc gagagcgccg atgttcggac
      901 ctcctcagcc tcgtgggaag gaggagccct tccgcagggc agcgagccag tggacctaga
      961 ccctgagaac ttcaccacca aaatcgatca tccctactgg ccgatggttc ccggcacgcg
     1021 gtggacgtac cgcgagaccg atcaggaggg cgccgagcta cgggtcgtag tcacggttac
     1081 aaagcagacc aagaagatcg ccaacggcat cacggcccgg gtcgtacgcg acacggtcac
     1141 cgaagacggc gagatagtcg aggacacctt cgactggtac gcccaggacg agcggggcaa
     1201 cgtctggtac atgggagaga acaccgccga gttcgaggac ggcgagctcg cgacaaaatc
     1261 cggctccttc gaagctggag tcgacggggc tcaacccggc atcataatgc ctgccaaccc
     1321 caaggacggg atgcagtacc gccaggagta ctacaaggat aaggcggaag acaacggtga
     1381 gatcctcagc acagacgaga tggccgaggt gccttacggc caattcgaca acgctctcct
     1441 gaccaaggac accataacta tcgagcctga cgtactggag tacaaacttt atgctccgcg
     1501 cgtcggaccg gtgctcgccg tgggcgtctc cggcggacca ggtagccgcg aggaactcgt
     1561 cgagctcgac gacgccagcg aagagtggac cagcaagtcc ggcacagcgc cgctcggcga
     1621 aggacccata gctcccgcaa agccataagc gcgcacatcc ctccgccggc ccgtagcacc
     1681 gcaatcctgg cgcggagcgg ctggcggacc catatatctt ctcggcgtac gccccctact
     1741 tcacgacgcg aaatctcagg tgcgtcaccc cgggagcctc gaccaccatg gttctctcca
     1801 gttcgatcgg tccggtgcgc agacggtcga aggagctgac gccctccccg agcaggacag
     1861 acaccaggtc gatgcctacc tcgtcgatca ggccggcccc gatgcactgc tgcgcgacgt
     1921 tggcgccacc cacgccgacg tccatgctcc cggccacgac cttcgcctgc tcgacggccc
     1981 tctcgacgcc gtccgtgacg aaggtgaagg gggagccttc gtgggttcat tctcggggaa
     2041 ccgcgtgggt gacgacgaag accggcacgt ccatcggatg cctgccgccc caggctccgg
     2101 cgtggtcgaa atgcctgcgt cccaccacca actcccccat cctcccgtaa gactcggaga
     2161 gaagctcggc gctttgcgcc gataccctga gcgtgaagtt gcctcctggc accgtgtact
     2221 cggtgtcgcc cgcgaagtac cagtcgaaga gcggcccgac atcgccgtac gggtgggcaa
     2281 tgaacccgtc cagggaagta gagaaccctg tagctacttt ccccatgctc gttccccttc
     2341 ctccgccgcg tcgtcgggtc cttatcggga ggccgtttgc ttcggctgcg ggctagtagg
     2401 gcatttgctg cagggtccac ccgttttcgt cgggatcgct gaagccagcg tacagtatcc
     2461 ctccgagatc ctgcacctcg cccacctcca ccccgcgttc cgagagctcg gcgcgcgccg
     2521 cctcgatgtc cgccaccacc aggtgcaacc cccggacgga gccgggcggc gtgtggacga
     2581 tccccgatcc caccgcgacg gagcaggcgg agcccggcgg cgtgagctgc acgacacgaa
     2641 atcctccctc gccgctatgg tcga
//
LOCUS       DBNGVD010000388         4592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1368214, whole genome shotgun sequence.
ACCESSION   DBNGVD010000388 DBNGVD010000000
VERSION     DBNGVD010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1368214"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1790)
                     /locus_tag="AC16CM_10465"
     CDS             complement(114..1790)
                     /locus_tag="AC16CM_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3140296.1"
                     /translation="MYQQIYEPIANNLVLSAVVALIPIIVLFVLLAGIRMAAQWASLI
                     TLASALFIAVVIYGMPFGLALNSTLLGICFGLFPIVWIVINAIFIYNVIVDTGHFDTI
                     RDSLAGVSNDRRVQVVLIAFVFGALLEATAGFGTPVAITASLMAGLGFEAVYAAALAL
                     LANTAPVAFGGFGIPILTAGAVSGLDVMELSQMVGRQTPLLALIIPGMLVTVMAGFRK
                     MLEVWPVVVVSGLAFALTQFLVSNLLGPELADLLAAIITVVAVVGLLAFWRPAEEWHF
                     EHEPPASERESYQTPPLGRTLYAWSPFIIIVVLFLIVQIPPIKAAVGATQTAINFPTA
                     EVSETTGLPTIPWPGLNGHVEQTEPVVPEPTPYDATFSTNWLSAAGTIILIGDIIALF
                     VLRVGPRRALRIYGESLNQLKWAILTIASILGLAYLLNYSGMTYTLGLTLAATGVLFP
                     FFSAFIGWLGVALTGSDTSSNALFANLQKVTAQQIGVSPNLTVGANSSGGVLGKMISP
                     QNLAVGTSATGLQGREGDLLRLVLKWSIGLTVVMAILVTLQAYVLPFMIP"
     gene            complement(1992..3293)
                     /locus_tag="AC16CM_10470"
     CDS             complement(1992..3293)
                     /locus_tag="AC16CM_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3140297.1"
                     /translation="MRKDLEKIWEAGLAAADPERAVGRALGMEDDAIVVDGARFVPEK
                     VFVVAVGKAAGTMAKAARELLGERISGGLVVTKDDHEPGPESFEVVFASHPEPDERGV
                     EAARRVQELAESAGEGDLLLALISGGASALLADPAPPIELADLKALTGDLLRSGADIG
                     EINTVRKHVSVLKGGGLARLAHPAPTVALLLSDVVGDDPSSIASGLTAPDTTTLEDTR
                     HVLRRYRIEPPESISEHLRDAEETPAEDDPVFENVSNVVCGGGRHASEAAAREASGLG
                     YTPLLLSTTLTGDALGAASVYAAIIREVLASGNPVSRPCAVISGGEATVTVRGDGEGG
                     PNQEFALALAVELDGVEGWAAFSADTDGHDGSTGAAGGIVDGRTAGNIRDNGVDPEES
                     LSDNDSSPALRAGGSLLITGPTGTNVNDLRVALVDDGTGRG"
     gene            3364..4197
                     /locus_tag="AC16CM_10475"
     CDS             3364..4197
                     /locus_tag="AC16CM_10475"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HIR3140298.1"
                     /translation="MRFSANVSILFKEVPFLDRFWRAREAGFSAVEFWWPSDEDLSEV
                     KGAIEEAGLEVALFNFDAGDMAAGDRGLAGDPGRQRQFRENVPVALDLARSLGCGRLN
                     ALVGHEVPGMAREEQLALARENVRFAAGEAGAAGIDIMVEAVNTFENGPYLLYTTGQA
                     VGFVRDVGRENVKVQHDFYHMQRMEGNLVANLREHIGLIGHVQIADSPGRGEPGTGEI
                     HYPFVLGELERLGYEGYVGLEYNPTTQTTEESLAWLPEELRGENVSVADLRLREGERD
                     G"
     gene            4190..>4592
                     /locus_tag="AC16CM_10480"
     CDS             4190..>4592
                     /locus_tag="AC16CM_10480"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140299.1"
                     /translation="MVDTVGFIGLGIMGKPMAENLVEAGYDLVVYNRTREKAEELDGA
                     TVAETPREVAERSDVIITMLPDSPEVEEVLAGEDGVLEGVREGALIVDMSTISPVVTE
                     ELAGRAEERGASMLDAPVSGGDVGAIDGTLSI"
BASE COUNT          809 a         1462 c         1568 g          753 t
ORIGIN      
        1 gaacctcgct atcaacccca cgtggacggc catcgcgacg accgccatca ccgtcgtggt
       61 gcgcaagctc ttgacgtact cgttgaatct gcagcggcgg gaggccggtc cgtctacggg
      121 atcatgaagg ggagcacgta ggcctggagg gtcaccagta tcgccattac caccgtgagc
      181 ccgatggacc acttcaggac caggcgcagg aggtcgccct ccctgccctg caggcccgtg
      241 gcgctcgtac ccacggcgag gttctggggc gagatcatct tgcccagaac gccgccggag
      301 gagttggcgc ccaccgtcag gttcgggctt accccgatct gctgggctgt gaccttctgc
      361 aggttggcga agagggcgtt agatgacgtg tccgagcccg tgagcgccac cccgagccac
      421 ccgataaacg cggagaagaa cgggaacagg acgcccgtgg ccgccagcgt caaccccagg
      481 gtgtaggtca tgcccgagta gttgagcagg taggcgaggc cgaggatcga agctatcgtc
      541 aggatcgccc atttgagctg gttgagggat tcgccgtaga tacggagcgc ccttcggggg
      601 cccaccctca gcacgaagag cgcgatgatg tccccgatca ggatgatggt gccggccgcc
      661 gagagccagt tggtcgagaa cgtggcgtcg tagggcgtcg gctcgggcac cacgggctcg
      721 gtctgctcga cgtgcccgtt tagaccgggc caggggatgg tcggaagccc tgtcgtctcg
      781 ctgacctcag cggttgggaa gtttatggcg gtctgggtgg cgcccacggc ggccttgatg
      841 gggggtatct gcacgatcag gaacagcacg acgatgatga tgaacgggga ccaggcgtag
      901 agcgtgcgcc cgagcggggg ggtctggtag ctctccctct cggatgccgg gggctcgtgc
      961 tcgaagtgcc actcctcggc gggcctccag aaagcgagca aacctaccac ggctaccacc
     1021 gtgatgatcg ccgccagcag gtctgccagc tcggggccga gcaggttgga gaccagaaac
     1081 tgcgtgaggg cgaacgcgag gccgctgacg actacgacgg gccagacttc cagcatcttc
     1141 cggaagccgg ccatcacggt caccagcatg ccggggatga ttagggccag gagcggcgtc
     1201 tggcggccga ccatctgcga gagctccatg acgtccagcc cggagacggc gccggccgtc
     1261 agaataggga tgccgaatcc gccgaacgcg accggcgcgg tgttcgccag cagggccagc
     1321 gccgcggcgt agacagcttc gaagccgagc ccggccatca gcgaggcggt gatcgcgacc
     1381 ggcgttccga accctgccgt cgcctccaga agcgccccga acacgaaggc gatgaggacc
     1441 acctgcacgc ggcggtcgtt gctgaccccc gctagcgagt cccttatggt atcgaagtgt
     1501 ccggtgtcca cgatcacgtt gtagatgaag atcgcgttta tgacgatcca cacgatgggg
     1561 aagaggccga agcagatgcc gagcagagta gagttcaacg ccagcccgaa cggcataccg
     1621 tagatgacca ccgcgatgaa gagggccgag gccagggtga tgagggaggc ccactgcgcc
     1681 gccatcctga tgccggcaag cagcacgaac aacacgatta tgggtatcag ggcgaccacc
     1741 gccgagagca ccaggttgtt cgcgatcggc tcgtaaatct gctggtacat caggctcccc
     1801 ttcctggtac gtgctatcga tgattctggc gcaatgtcta cctactgatc ccagcacgca
     1861 atatggcacc atcgcatccc agacgcaagc ctcccgggca agctcggcgc gaactcgcga
     1921 aaagtgcgaa gtcgggcgac ggctggtatt aggtcttgat gctcaagaac cgtcgccttc
     1981 tgccgggtgg ttcaccctct ccccgtgcca tcgtctacga gcgccacccg caggtcgttg
     2041 acgttggtgc ccgtcgggcc ggtgatgagc agagatcccc cggcccggag cgcgggtgag
     2101 gaatcattgt cggacaacga ctcctcgggg tctacgccgt tgtcccttat gtttcccgcc
     2161 gtccggccgt caactatgcc gcccgccgcc ccggtcgagc cgtcgtggcc gtcggtgtcg
     2221 gcggagaagg ccgcccagcc ttcgacgccg tccaactcta ccgccagcgc gagcgcgaac
     2281 tcctggtttg gaccgccctc tccatccccg cgcaccgtca cggtcgcctc tccgccagag
     2341 atcacggcgc agggcctgga cacagggttg ccgctcgcca gcacctccct gattatcgcc
     2401 gcgtaaaccg aggctgcccc cagggcgtcg ccggtgaggg tggtggagag caagagcggc
     2461 gtgtagccga gcccagacgc ctcacgggcg gcggcctcgg aggcgtgacg gccgccaccg
     2521 cagaccacgt tcgagacgtt ctcgaagacg gggtcgtcct ccgccggggt ctcttccgca
     2581 tcccgcaggt gctccgaaat gctctcgggc ggctctatcc ggtagcgcct caggacatgc
     2641 ctcgtgtctt cgagggtggt ggtgtctggg gcggtgaggc cgctcgcgat cgaggaagga
     2701 tcatcgccca ccacgtcgga gagcaggagt gccacggtgg gggccgggtg ggctagcctc
     2761 gccaggcccc cacccttcag gaccgagacg tgttttctga cggtgttgat ctcccctata
     2821 tccgcaccgc ttctgagcag gtctcccgtc agcgctttca ggtcggcaag ctcgatgggc
     2881 ggggccgggt cggccagcaa agcggaggcc ccgccggaga tcagggccag aaggagatct
     2941 ccctccccgg cggactcggc cagctcctgc acccgcctgg ctgcctcgac gcccctctcg
     3001 tcgggctcgg ggtgggaggc gaagacgacc tcgaagctct cggggcccgg ctcgtggtcg
     3061 tctttggtca ctacgagccc gccggagatc ctctcgccga gaagctcccg cgcggccttc
     3121 gccatcgtcc cggcggcctt gccaacagcc accacgaaca ccttctcggg tacgaaccgc
     3181 gccccgtcga ccacgatggc gtcgtcttcc atcccgagcg cccgccccac ggcccgctcg
     3241 gggtccgcgg ccgccagccc ggcctcccat atcttctcca ggtcctttcg cacgggctca
     3301 ttgtagcgtc ccgcatgcca cgcgcctgct ttagggtatg ctggtgaagg aaggaggggc
     3361 accgtgaggt tcagcgcgaa cgtttccata ctcttcaaag aggtcccgtt ccttgaccgg
     3421 ttctggcggg cgagagaggc tggcttctcg gcggtcgagt tctggtggcc gtctgacgag
     3481 gatctctcgg aggtaaaagg agccatcgaa gaggctggcc tcgaggtcgc tctcttcaac
     3541 ttcgacgccg gcgacatggc ggccggtgac agggggcttg ctggggatcc cgggcggcag
     3601 cggcagttca gggagaacgt gcccgtggcc cttgacctcg cccgctccct ggggtgcggg
     3661 aggttgaacg cgctcgtggg tcacgaggtc cccggcatgg cccgcgagga acagctggcg
     3721 ctggcgcgcg agaacgtccg tttcgccgcc ggggaagccg gggcggcggg catagacatc
     3781 atggtcgagg ccgtaaacac cttcgagaac gggccctatc tgctgtacac gaccgggcag
     3841 gccgttgggt tcgttcggga cgtgggacgg gagaacgtaa aggttcagca cgacttctac
     3901 cacatgcagc ggatggaggg gaacctagtg gccaacctgc gcgagcatat cggcctgatc
     3961 gggcacgtcc agatcgccga ctcccccggc cgcggggagc caggcacggg cgagatccac
     4021 taccccttcg tgctcggcga gttggaacgg ctcggctacg agggttacgt cggcctcgaa
     4081 tacaacccga ccacgcagac cacagaggag agcctggcct ggttgccaga agagttgcgt
     4141 ggcgagaacg tttcggtggc tgacttgagg ctcagggaag gggagagaga tggttgatac
     4201 ggtaggtttc atcgggctcg ggatcatggg caaaccgatg gcggagaacc tcgtcgaggc
     4261 gggctacgac ctggtggtgt acaaccgcac ccgtgagaag gccgaggaac ttgacggtgc
     4321 taccgtcgcg gagaccccga gggaggtcgc ggagcggtct gatgtaatca tcaccatgct
     4381 gcccgactcg cccgaggtcg aggaggtgct cgccggggaa gacggcgtac tggagggcgt
     4441 cagggaaggc gccctgatcg tggacatgag cacgatctcc cccgtcgtca cggaagagct
     4501 ggccggtagg gccgaagaac gtggcgcctc tatgctcgac gcgcctgtca gcggggggga
     4561 cgtgggggcc atagacggga cgctctccat ca
//
LOCUS       DBNGVD010000389         5315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4283240, whole genome shotgun sequence.
ACCESSION   DBNGVD010000389 DBNGVD010000000
VERSION     DBNGVD010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4283240"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16CM_10485"
     CDS             complement(<1..461)
                     /locus_tag="AC16CM_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3140300.1"
                     /translation="MTLAQISQDELREKILHTFEVARHAWHIPLHIGPIDISINKAVW
                     FLWIGAAVTFLLLFIGSRLLKDRPGAYQVIVEEVYGFGRNLGGQVGDEGKKWFPYTLS
                     LFVFILTLNLIGLIPNTYPVTANISFTLMLAFLTFVLTQYEGFRRNGAGAYL"
     gene            complement(464..907)
                     /locus_tag="AC16CM_10490"
     CDS             complement(464..907)
                     /locus_tag="AC16CM_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140301.1"
                     /translation="MTGGHWRLAFRCAGWIVALMVPVAAVLLYFYGMGHAEAFAYGVG
                     IGLVSLLSTALTVSLLWGPSMSLGMMVGAFSFVARCGLAAGALGIPAYLGLWPVLPML
                     GGFAGVYLVENILLMPGALKISHAGIKQESERLKREAEVERRVEI"
     gene            complement(904..1164)
                     /locus_tag="AC16CM_10495"
     CDS             complement(904..1164)
                     /locus_tag="AC16CM_10495"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR3140302.1"
                     /translation="MQHIGPYGMMRVMPDTRSQRNDADGNYARFVGLGVTLLLIFALP
                     TVVGFFLDRLAGTLPLFLLIGLALGFVGGMYYVYRALQKLGG"
     gene            1447..2082
                     /locus_tag="AC16CM_10500"
     CDS             1447..2082
                     /locus_tag="AC16CM_10500"
                     /inference="COORDINATES: protein motif:HMM:NF044444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140303.1"
                     /translation="MSGERVRRRRRKPEEAERVILAAGRAFLEEHPFREMTVEGVMVR
                     TGLSRPAFYAYFEDRYDLVTHLLDGVGGLLFALDWRWLSGTAGGEDAREVLVDGLRAR
                     SETFVEYGPVLRAISDAAGYDARVEEVYRGGLIQRLVDAVAARISRDMEAGVTPGELD
                     PEQTAQALVLMTERYLLDAFGRPERRPSHQEAEVVLDTVEEIWVRTLYGPE"
     gene            complement(2144..3913)
                     /locus_tag="AC16CM_10505"
     CDS             complement(2144..3913)
                     /locus_tag="AC16CM_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3140304.1"
                     /translation="MTEGVFRPTTRARRSIGRLLRVLLVLGWALLVALSYEPAAAAAT
                     TFTVTETGDDADINLANNRCDSSTSTGDQCTLRAAIEEANDTTGADIIDFDIGGSVSG
                     KTISVGSPLPTIAEAVTIDGYTQAGASPNTLIEENDAVLNVVLGGLGAGANSDGIVIQ
                     ASDCTVKGLEIKNFGGAGVKIVGVGGTGTRIEGNFLGIDRNGTTDRGNSYGVVIEGGS
                     STVGGTEPASHNIISGNDQSGVYISGGTGNKIEGNLVGTTADGEGALGNGYGVQIDSA
                     DNMVGGTVAGARNVISGNGSYGMFIGPEARDTLIKGNYVGPTADGGGDLGNDSHGLYV
                     QGDSNTVGGRTTGARNVISGNDGFGVFVSGRFGGNENRIEGNFIGTTADGEGDLGNAR
                     GGVTIQNGPDFTAVGGTLPGAANRIAYNGEDGVTVADNGDATGNNILSNRIYDNDELG
                     IDLVGGAENANGVTSNDTDDPDTGSNNLQNFPTITLATKSLALGQTIISGRLNSTPTQ
                     DFVVQCFLTEGAPGSDFGEGWRLLDTVPISTNANGRATFICESSRMLLGLQPGQTVSA
                     TATNVATGDTSEFSKNKTIISGP"
     gene            complement(4050..4547)
                     /locus_tag="AC16CM_10510"
     CDS             complement(4050..4547)
                     /locus_tag="AC16CM_10510"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR3140305.1"
                     /translation="MEPFKDPSTDTTRSHETLKKVRPSWDEYFMRIAHEVATRSTCPR
                     LAVGAVVVRDKRILTTGYNGSPSGMPHCDEVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVRITGASIYSTHQPCLMCVKMIKNAGIEEVHFAGGYPDPLAMELATEGGLAMLR
                     FTPGP"
     gene            complement(4534..5160)
                     /gene="upp"
                     /locus_tag="AC16CM_10515"
     CDS             complement(4534..5160)
                     /gene="upp"
                     /locus_tag="AC16CM_10515"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3140306.1"
                     /translation="MENLRVVDGPLSRHKLAVLRDERTKTPDFRQAMKELALIMVAEA
                     TGRMPTKQVRIDTPLTETEVEEISGPVCLVPVLRAGLGMLDGALALLPTATVGFMGLQ
                     RDEETAQPVEYYVNLPRNLEEYLVLVLDPMLATGGSLSATIDKLKDEGATWISCLHAV
                     AAVPGVERLTRDHPDVTFYAAAVDPELDDRSFIVPGLGDAGDRLYGTL"
BASE COUNT          972 a         1647 c         1654 g         1042 t
ORIGIN      
        1 aggtacgcgc ccgcaccgtt ccgtctgaac ccctcgtact gggtaaggac gaacgtgaga
       61 aaggccagca tcagcgtgaa cgagatgttg gcggtcacag ggtacgtgtt ggggatgagc
      121 ccgatcaggt tcagcgtcag gatgaacacg aaaagcgaga gcgtgtaggg gaaccacttc
      181 ttgccctcgt cccccacctg cccgccgagg ttgcgcccga aaccgtagac ctcctcgacg
      241 atgacctggt aggcgccggg cctgtctttc agcaaccggc tgccgataaa caggagcagg
      301 aacgtgaccg cggccccgat ccacaagaac cagacggcct tgttgatgga aatatctatg
      361 ggcccgatgt gcaatgggat gtgccaggca tgcctggcga cctcgaacgt gtgcaggatc
      421 ttctcccgca gctcatcctg gctgatctgg gccagagtca ccgtcaaatc tccaccctcc
      481 gttctacttc cgcttcacgc ttcaaccgtt cggactcctg cttgatgccg gcgtggctga
      541 tctttagtgc cccaggcatg agcaggatgt tctctaccag gtaaacgccc gcaaaccccc
      601 cgagcatcgg cagcacgggc cacagcccga ggtatgcggg tatacctagc gccccagccg
      661 cgagcccgca ccgggccacg aacgagaacg ccccgaccat cataccaagg ctcatgctcg
      721 gcccccacaa cagcgagacc gtaagggccg tggagagcag gctgacaagc cctatcccta
      781 caccgtaagc gaaggcttcg gcatggccca ttccatagaa gtagagcagc accgccgcga
      841 cgggaaccat caacgcgacg atccacccgg cgcaccggaa tgcaagccgc cagtgcccgc
      901 ccgtcatccg cccaacttct ggagcgcccg atatacgtag tacatccccc cgacgaaacc
      961 caacgctagc ccgatcagca gaaacagcgg caacgtcccg gccaatctgt ccaggaagaa
     1021 acccaccacc gtagggagag cgaagatcaa gagtagcgta accccaagac ctacgaaacg
     1081 cgcgtagttg ccgtcggcgt cgttccgttg agatctggtg tcgggcatca cgcgcatcat
     1141 accgtatggg cctatgtgtt gcaatcagtg tcaatctccg gaaaacacct ccccagaagg
     1201 atcgccgcaa ggccggatcg tgccacaccc aagtgagcct ggatgtgcaa caagagaaat
     1261 cccggtcgag tgccgcatca cgtcgcactc cagacgtaac cgcctctgcg taccggtctc
     1321 aaccgtgccc tcctctcgtg gcttcgtaac agcatgatac tgttttattg acgtgctgtc
     1381 aatagacacg gtgtcaataa aggagtacat tgtgcccgtg gagaaggatc caaaggtggc
     1441 ggtgaggtga gcggagagcg ggttcggcga cggcggcgca agccggagga ggcggagcgg
     1501 gtaatactgg ctgccgggcg cgcgtttctg gaggagcatc cgttcaggga gatgaccgtt
     1561 gagggtgtca tggtaaggac ggggttgtcg cggcctgcgt tctacgcgta tttcgaggat
     1621 cgttacgacc tggtaacgca cttgctcgat ggtgtggggg ggctgctctt cgcgctagat
     1681 tggcgctggc tgtccggtac ggcgggcggg gaagacgcga gagaggttct cgtcgatggg
     1741 ttacgcgccc gctcggagac gttcgtcgag tacgggccgg tgttgagggc catctccgac
     1801 gccgccggct acgacgccag ggtcgaggag gtctacaggg gaggtttgat ccaacgcctc
     1861 gtggacgcgg tggcggcccg catctcacgc gacatggaag ccggcgttac ccccggagag
     1921 ctagaccccg aacagacggc gcaggcgctc gtgctcatga cggaacgcta ccttctcgac
     1981 gcgtttgggc ggccggagag gcgtccttcg catcaggagg cggaggtcgt tctggatacg
     2041 gtagaagaga tctgggtaag gacgctgtac ggccccgagt aacgaggtag gggcaggtct
     2101 ttatacaccc ctaccggcga atgtgggtag ggcgtcctgg ggtttacgga ccgctgatga
     2161 tcgtcttgtt cttggagaac tccgaagtat caccagtcgc cacgttggtc gccgtcgcgc
     2221 ttaccgtttg ccccggttgc agcccgagga gcatgcgcga gctctcacag atgaaggtcg
     2281 ccctcccgtt agcgttggtc gagatgggca cggtgtcgag aaggcgccaa ccctcaccaa
     2341 agtcggagcc cggcgcgcct tccgtcagga agcactgaac gacgaagtcc tgggtggggg
     2401 tactgttgag cctaccagag atgatcgttt gccccagcgc gaggctcttc gttgccaggg
     2461 ttatggtggg gaaattctgg agattgttgg aaccggtgtc gggatcatcg gtgtcgttcg
     2521 acgtaacccc gtttgcgttt tcggcaccac ccaccaagtc tatgccaagc tcgtcgttat
     2581 cgtagatgcg attggagagg atgttgttgc ccgtcgcgtc gccgttatcg gcaaccgtca
     2641 ccccgtcttc gccattgtag gcgatgcggt tggccgcccc ggggagcgtg ccgccgaccg
     2701 ctgtaaagtc cggcccgttc tggatggtga cgcctcctcg ggcgttgccc agatcacctt
     2761 caccatcggc cgtggtgcca ataaagttgc cttcgatcct gttctcgttt ccaccaaaac
     2821 ggccggagac gaagacgccg aacccgtcgt ttccagagat gacgttgcgt gctcccgtcg
     2881 tcctacctcc gaccgtgttc gaatcgccct gaacgtacaa gccgtgcgag tcgttaccca
     2941 gatcacctcc accatcggct gtagggccca catagttccc ctttatcaaa gtgtccctcg
     3001 cctcgggacc gataaacatg ccataactgc cgttcccgga gatgacgttg cgcgcccctg
     3061 ccactgtgcc accgaccatg ttgtccgcgc tatcgatctg cacaccgtat ccgttgccca
     3121 gtgcaccctc cccgtccgcc gtggtgccta ccaggttacc ctcgatcttg ttgcctgtgc
     3181 ccccgctgat gtagacgccg ctctggtcgt tgccggagat gatgttgtgg gatgccggct
     3241 ctgtaccacc gaccgtgctc gaaccgccct cgataacaac accgtatgag ttgccgcggt
     3301 cggttgtccc gttcctgtcg atgcccagga agttaccctc gatgcgggtg ccagttccgc
     3361 ccacaccgac gatcttgacg ccggctcccc caaagttctt tatttccaga cccttcaccg
     3421 tacagtcgga ggcttgaatc acgatcccgt cggagttcgc ccctgcaccg agcccgccga
     3481 gcaccacgtt gaggaccgca tcgttttctt cgatcagggt gttggggctt gctcctgcct
     3541 gcgtgtagcc atctatggtg accgcctcgg cgatggtggg taaaggagag ccgacagata
     3601 tggtcttgcc cgagacggag ccgcctatgt cgaagtcgat gatgtcggct cccgtcgtat
     3661 cattggcctc ttctatagcc gccctcaagg tgcactggtc cccggtagat gtcgaactgt
     3721 cgcacctgtt gttggcaagg ttgatgtccg cgtcatcacc cgtctcggtg acggtgaacg
     3781 ttgtggcggc cgcggcagca ggttcgtacg aaagcgcgac gagcaacgcc cagcccagca
     3841 ccaggagcac gcgcagcaac ctcccgatcg atcttcttgc cctggtcgtt ggccggaaca
     3901 caccttcggt caccgttacc tcgctcccgt tgcttcacat cgtgatgtaa cacacatgat
     3961 ttgcattcca ctaatagcag cggtggggcg agagcgcatc gcacaaatga atgattctct
     4021 cctggcgagg gacggttcga gcggagctgt tacgggccgg gcgtgaaccg gagcattgcg
     4081 aggccgcctt cggtggcgag ttccatcgcc agggggtccg ggtatccgcc cgcgaagtgg
     4141 acctcttcta taccggcgtt cttgatcatc ttcacgcaca tcaggcacgg ctggtgcgtg
     4201 gagtagatcg aggcgcccgt aattcgcacg ccgtggtagg cggcctggat gatggcgttc
     4261 tgttcggcgt gcagcgtgcg ctggcagctc tcacggccgt ctacgatgcg gatcaggcac
     4321 cccacctcgt cgcagtgggg cattcccgag gggctaccgt tgtatccggt ggtcagaata
     4381 cgtttgtcgc gcactaccac ggcgcccacg gcgaggcggg ggcaggtgga gcgggtcgcg
     4441 acctcgtggg ctatgcgcat gaagtactcg tcccaggaag gacggacctt cttcagggtc
     4501 tcgtgggagc gcgtggtgtc agtcgagggg tccttaaagg gttccataga gcctgtctcc
     4561 tgcgtcgcca aggccaggca cgatgaacga gcgatcgtcc agttccgggt cgacggccgc
     4621 ggcgtagaac gttacatcgg ggtgatcccg ggtgaggcgt tcgacgccgg gcaccgcggc
     4681 gacggcgtgg aggcaggaga tccaggtggc tccctcatcc ttcagcttgt ctatggtagc
     4741 cgagaggctc ccccccgtcg caagcatggg gtcgagaacg aggaccagat attcctcgag
     4801 gttgcgtgga aggttgacgt agtactcgac gggctgggcg gtctcctcgt cccgctgcag
     4861 tcccatgaaa ccgaccgtag ccgtcggcag cagggcgagg gcgccgtcga gcatgccgag
     4921 cccggcgcgc aggacgggca cgaggcagac cgggccggag atctcctcga cctccgtttc
     4981 cgtgagcggc gtgtcgattc ggacctgctt cgttggcatc ctgcccgtgg cctcggccac
     5041 catgatgagg gcgagctcct tcatggcctg cctgaagtcc ggggtcttgg tacgctcgtc
     5101 tcgcaggacg gccagcttgt ggcggctcag ggggccgtct acgacccgga ggttctcgat
     5161 attcggcgtg gtctcactca tagaggggat acgcctccgt cagggccgtc accttgtcgc
     5221 gcaggccatc catctcgccg cgggtcgtcg ccacgatgat ctcggcgatc tcgcgcatct
     5281 cgccctcgcc catgccgcgg gtcgtcatcg ccgcc
//
LOCUS       DBNGVD010000390         1922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_638041, whole genome shotgun sequence.
ACCESSION   DBNGVD010000390 DBNGVD010000000
VERSION     DBNGVD010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_638041"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1887)
                     /gene="hutH"
                     /locus_tag="AC16CM_10520"
     CDS             complement(337..1887)
                     /gene="hutH"
                     /locus_tag="AC16CM_10520"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR3140307.1"
                     /translation="MRCTQNRGKGSVRVEDLDPASVVAVARRGVRLTLGAEARERMRS
                     SRKLIEDVIEDSLPVYGVTTGFGALANTRIAAHERLELQNSLLRSHAAGMGTFVEGEV
                     VRAMMAIRVRTLAAGYSGVRPELVDALVDLLNNGLTPAVPEHGSMGASGDLAPLAHIG
                     LCLIGEGLMLVDGEPVQGGRALQEAGLQPFRPATKEGLSLINGTDGMLAMLLLAQEDL
                     SLLLKTADVTASMSVEALLGTDRVFAEALHLLRPHPGQLASARNITRVLSGSPIVASH
                     RDSPHLVQDAYSLRCAPQVYGAARDTLEHARLVAARELDSVTDNPVVLPDGRVESAGN
                     FHGEPLAFVLDFLAIAASEIGAITERRIDRMLDPRRSEGLPAFLSPRPGVNSGFMIPH
                     YTAAALAEENRRLANPASTGSLPTSAAQEDHNSMGWSAGLKLRRLLENVSRILAVEAA
                     CAAQALDLRRPLTPGLVTGAVLRRIRQEVPFIEEDTFLAPHLQTAESMVRSGAILAAA
                     EEKIGPLD"
BASE COUNT          304 a          621 c          664 g          333 t
ORIGIN      
        1 gatgacgtcc atccgtcgtg gtccgacccc gtcgtcgatg gcacgcccga tcaggagcgg
       61 cgtcatcaag ttggccgctg agaccaggag cagcgatacg actgcacccc cgctctcccg
      121 cttgtaggag cgcagatagg acagcgcccg ccgcagcgcg cttgcccccg acggtgtccc
      181 cccgtcggcc agaaccccta cccgttcctc tcttgcgtga tcacgccttc tccctgtcgg
      241 agccgccgac gttatggtat ccgatcctgc cgaaacgatg ggttaggaag gcaacaggct
      301 tcagggagat tacgcgttgc ctgatgcctg tagtctctag tcgagcgggc cgatcttctc
      361 ctcggcagcc gccagaatgg ctccggatct caccatgctc tccgccgtct ggaggtgtgg
      421 ggccaggaag gtgtcctctt cgataaacgg aacctcctgc ctgatgcgcc gcagaacggc
      481 gccggtgacc aggccggggg tgaggggcct gcgcaggtcg agagcctggg ccgcgcaggc
      541 ggcctcgacg gcaagtattc gcgagacgtt ctcgaggagg cggcgtagct tgagtccggc
      601 gctccagccc atggagttgt ggtcttcttg cgcggccgag gttgggaggg agccggtgct
      661 cgcggggttc gcgagccgac ggttctcttc cgcgagggcg gccgcggtgt agtgggggat
      721 catgaacccc gagttgacgc cgggccgtgg gctgaggaag gcgggaaggc cctcggagcg
      781 cctggggtcg agcatgcggt ctattctgcg ctcggtaatg gcccctatct ccgaggcggc
      841 gatggccagg aagtcgagca cgaaggcgag cggctccccg tggaagttgc ccgcgctctc
      901 cacccgcccg tcgggcagga cgaccgggtt gtccgttaca gagtcgagct cgcgggcggc
      961 aaccagccgg gcgtgctcca gggtatcgcg cgccgcgccg tacacctgcg gggcacagcg
     1021 caacgagtaa gcatcctgga ccaggtgcgg agagtcgcgg tgtgaggcga cgatggggct
     1081 gccggagagc accctggtta tgttgcgggc gctcgctagc tggccggggt gggggcgcag
     1141 gaggtgtaga gcttcagcga acaccctgtc ggtgcccagg agggcctcga cggacattga
     1201 cgccgtgaca tccgcagttt tcaggagaag gctgagatcc tcctgtgcga gaagcagcat
     1261 cgcgagcatg ccgtcggtgc cgtttatgag ggacaggccc tctttcgtgg ctggccggaa
     1321 gggttgcaga ccggcctctt gcagggccct accgccctgt actggttcgc catccacgag
     1381 catcaggccc tcaccgatca ggcacaaccc gatgtgggcg aggggggcga ggtcgccgct
     1441 ggcacccatg gagccatgct cgggaacggc cggggtgagc ccgttgttca ggaggtccac
     1501 cagcgcgtct accagctcgg gacggacgcc cgagtagccc gcggcgagcg tcctgaccct
     1561 aatggccatc atggcacgca cgacctcgcc ttccacgaag gtccccatcc cggcggcgtg
     1621 gcttcggagg agagagttct gaagctccag gcgctcgtgc gccgcaatcc ttgtgttcgc
     1681 cagggcccca aaacccgtcg tgaccccgta gacggggagg gagtcttcga tcacgtcctc
     1741 gatcagtttc cgcgaggagc gcatgcgctc tctggcctct gcgcccaacg ttaaccgcac
     1801 gccccgcctc gccacggcga cgacagaggc ggggtcgagg tcttctaccc tgacggaccc
     1861 tttgccccgg ttctgcgtac agcgcatcag ggttcttccg gggaaccttc ttcctgctgc
     1921 cc
//
LOCUS       DBNGVD010000391         7727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3190045, whole genome shotgun sequence.
ACCESSION   DBNGVD010000391 DBNGVD010000000
VERSION     DBNGVD010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3190045"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1542)
                     /gene="accC"
                     /locus_tag="AC16CM_10525"
     CDS             complement(163..1542)
                     /gene="accC"
                     /locus_tag="AC16CM_10525"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3140308.1"
                     /translation="MVSKVLVANRGEIALRVIRACRELGVRSVAVYSTADRDSLHHEL
                     ADESVCIGPPPAVSSYLNIPAIISAAELTGADAVHPGYGFLAENARFAEICERVGLTF
                     VGPSSRTIAAMGDKAEARETAARAGVPVTPGSDGTCESAGEVKRVGAEVGYPLVIKAS
                     AGGGGKGMRVVESEAEVEAAFLGASREAGATFGDGSVYVERYLERLRHVEMQVLADSH
                     GTTVTFPERDCSVQRRYQKLIEESPAPGFPEEVRDGMMEASERLVGSLGYEGAGTVEF
                     VYAEGEFYFIEMNTRLQVEHPVTEMVSGVDIVAEQLKVASGGRLEIPPEALTPEGHAI
                     EFRINAEDPGRNFLPQAGLVEVYNPPGGPGVRVDSHLYAGYRVPPHYDSLLAKLIVHS
                     RDREGAVRRGLRALDEFAISGMETTLPLHLAILEDEEFLRGGVTTSFLAERTLESEGG
                     LLRLNKVPV"
     gene            complement(1542..2009)
                     /gene="accB"
                     /locus_tag="AC16CM_10530"
     CDS             complement(1542..2009)
                     /gene="accB"
                     /locus_tag="AC16CM_10530"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3140309.1"
                     /translation="MPEIEENPNLPLGEVERLVELLRESGVGEILVRRGETEISVKAK
                     PEAPVAQQAAPVAQGVPEMVEPETSPEMEGLHAVRSPVVGTFYRAPAPGEGAYVEVGD
                     AVSAGQTLCIVEAMKLMNEIVADVSGEVVEVLAEDAGGVEYDQPLFYLRPEAG"
     gene            2073..3911
                     /gene="mutL"
                     /locus_tag="AC16CM_10535"
     CDS             2073..3911
                     /gene="mutL"
                     /locus_tag="AC16CM_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3140310.1"
                     /translation="MAEKETGRARIKVLDPRVAQQVAAGEVVDRPASVVKELVENALD
                     AGASRVEVELGDGGTSRILVRDNGSGMTPENARLCVIRHATSKIRNVEDIEAVMTLGF
                     RGEALPSVASVSSFTLTTSTGEGAATRVFVDGGAEAEVSAASHPKGTTVLVDRLFYNV
                     PARRAFLKGARAERAAIVEVSTHLAIAHPTVAFRLTEGGRDYLSLPEAGDLLERLAQI
                     LGVGKARALRRVEYESGPFGVSGYAALPSLTEGSRAHQTISVNGRFVRAEGLHRGLDD
                     AYRATLPSGRYPPVALEITADPRRVDVNVHPTKQVVRFSEERAAREAVAAAVRGAIEW
                     RPSSPPSAPRPPERAFTQDRFSGYSRPRTDYPSRAAEDPSPYDAPPARSLQEARERLS
                     EASRPLEQPPTGERYDAEPYTDLPERGGLPRSAPEELRVIGQLAAGYILMEEPESLWV
                     VDQHVAHERAILDRLHDSESPATVQSLLVPEVVDLSPAEAEFAAASLEELSVYGFEAE
                     PFGPRSVRVTAVISTLAERDVAGAFKDALSAISGTEPGHRREDHILATIACHSAVKMG
                     DRLSPQEMEALVKSWLTSRLPATCPHGRSICYRIGLNEIARKLDRH"
     gene            complement(4054..4272)
                     /locus_tag="AC16CM_10540"
     CDS             complement(4054..4272)
                     /locus_tag="AC16CM_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140311.1"
                     /translation="MGSTYEAQQTRYEKGDRVWVLRGGAAREPGVVIGHHVDWNLYRV
                     VYTVELADRGPRAIEEWRLSFRRKGEEH"
     gene            4392..5309
                     /locus_tag="AC16CM_10545"
     CDS             4392..5309
                     /locus_tag="AC16CM_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3140312.1"
                     /translation="MDPTRDRILGVLAVVTAASIWATLGFFAKILYARGVSFESLVAV
                     RASVGWVAVMTFVLATGRARSLRVSRRDLAFLAPLGLFGIGAFYLFYFYTVRESTVGT
                     AAVLNYTSPAFVVVLAYLFLQEGLNAAKAAALLLTAGGIFLVAGAYDPSNLEVTPTVL
                     LTGLLSGLTYGLYSIFGRPVTGHLSPATILSYALFFGSSLLVVAALPTLHTLAGLPAS
                     SYAMLFMLSVVHTTLAFALYTFGLGRLGAGRAAIVATVEPVVAVALGTALLGEALTVP
                     KLVGASLVVLGAALAQVKLRKRPTRNADS"
     gene            5418..6527
                     /locus_tag="AC16CM_10550"
     CDS             5418..6527
                     /locus_tag="AC16CM_10550"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3140313.1"
                     /translation="MPIEFKEGNSGYTLGVEEELSIVDATTGELVPRIEEIMSRLPEP
                     LKEAVSYELFQSVLEIKTSVCDTVAETERQLRELRGRVGSWAAACGSSLASAGTHPFS
                     RYRDQKVTDQERYKNVIRTLRWIAQREVIFGQHVHVAVPGPEEAIIAHNRLSELAPLL
                     LALSTNSPFWQGFDTGFESSRVKIFEGFPRAGLPPAFPEYEAFEEYVDLMVAAGAMED
                     YTYCWWDVRPHPGIGTIELRVLDSQTSLKYTTYLTALTRCLVAEVLGEVGPHKPYNPH
                     LTLENKWRAARHGMDAAFYDAELDRTIPARDLARTLVERLQPHAKDLDCEDDLEGILE
                     IVEGGTGSQHQRRVYEKSGDFLDVVAFLIEGTRPT"
     gene            complement(6805..7083)
                     /locus_tag="AC16CM_10555"
     CDS             complement(6805..7083)
                     /locus_tag="AC16CM_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018024132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3140314.1"
                     /translation="MVTAIVLVRTERERVQEAAQAMLDIEGVTEAYSVTGPYDLVVMV
                     RIPDFESLAEIVPEKIAQVPGVARTETMVAFRAYSNYDLDRAWSIGFE"
     gene            complement(7096..>7727)
                     /locus_tag="AC16CM_10560"
     CDS             complement(7096..>7727)
                     /locus_tag="AC16CM_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140315.1"
                     /translation="RSQATNTLAQAASGDEEGFEKDLATAVADYRTSDTIIADFYVPA
                     VKRSLETAGQTGDQTYLQEPQSFMDYGALGFDAAPTTGTAESDPNALHGVQVTSVQVA
                     GKPLRQDGQVVLTGPDEPTFAVRVTNGGEVTETGIEVEVIMNTAAERQAQSETISRLD
                     AKKSTTVEVGGFLPGELDETARVSIKVGPVEYEKATSNNTLTGAVKFGI"
BASE COUNT         1250 a         2659 c         2478 g         1340 t
ORIGIN      
        1 cccccgcgtc gtctctagcc acgattcctc tccagtaggg tcaaagctca gaggttaaca
       61 cgcttgtaga gctgtcagca gtcagcggtc agctttcagc caaaaacaaa aagctaatgg
      121 ctgaccgctg actgcgcggt ctgggcctgt ggaccagacc gcttaaaccg gcaccttgtt
      181 gaggcggagc aggccgcctt cgctctccag ggttctctcg gcaaggaagc tggtggttac
      241 gcctccccgg agaaactcct cgtcttcgag gatcgccagg tgaagtggga gggtggtctc
      301 cataccggag atggcgaact cgtcaagggc gcggaggccg cggcgcacgg cgccctcgcg
      361 atcgcgggag tgcacgatca gcttggccag aagcgagtcg tagtgcgggg gcaccctgta
      421 gccggcgtac aggtgcgagt cgaccctcac gccgggcccg ccaggcgggt tgtatacctc
      481 caccagaccg gcctggggca ggaagttgcg gcccgggtcc tcggcgttga tcctgaactc
      541 tatagcgtgc ccttctgggg tgagcgcctc cggggggatc tcaagccggc cgcccgaggc
      601 gaccttgagc tgctcggcca ctatgtccac gccagagacc atctccgtca cggggtgctc
      661 gacctggagg cgcgtgttca tctcgataaa gtagaactcc ccctccgcgt agacgaactc
      721 cacggtcccg gcaccctcgt aaccgagcga gccgacgagg cgctcggagg cctccatcat
      781 gccgtccctc acctcttcgg gaaagccagg cgccggggac tcctcgatca gcttctggta
      841 gcgccgctgg acggagcagt ccctctcggg gaaggtcacc gtggtgccgt gcgagtcggc
      901 aagcacctgc atctcgacgt gcctgagcct ttcgaggtac ctctccacgt agacggaacc
      961 gtcgccgaag gtggcgcctg cctccctgct cgcgcctaga aaggcggcct cgacctcggc
     1021 ctccgattcg acgacgcgca ttcccttgcc accgccgccg gcgctcgcct tgatgaccag
     1081 cgggtagccg acctcggcgc cgacgcgctt cacctccccg gcgctctcgc aggtgccatc
     1141 tgagcccggg gtaacgggaa cgccggcccg ggcggccgtc tcgcgggcct cggccttgtc
     1201 gcccatcgcg gcgatggtgc gggagctagg cccgacgaac gtcaggccta cccgctcgca
     1261 gatctccgcg aagcgggcgt tctcggccag aaagccgtag ccaggatgca cggcgtcggc
     1321 gcccgtcagc tcggcggcgg agatgatcgc tggtatgttc aggtagctgc tcactgccgg
     1381 cggcgggcct atgcataccg actcgtcggc cagctcgtgg tgcagggagt ctctgtcggc
     1441 ggtggagtac acggcgacgc tacgcacccc aagctcccgg caggcccgga tcacacgcag
     1501 ggcgatctcg cccctgtttg ccacgagtac cttgcttacc actaccctgc ttcggggcgc
     1561 aggtagaaga gcggctgatc gtactcgacg ccgcctgcgt cctcggccag aacctcgacg
     1621 acctcacctg agacgtcggc cacgatctcg ttcatgagct tcatggcctc gacgatgcag
     1681 agggtctgac ccgcgctcac ggcgtcccca acctccacgt aggcgccctc accgggggcc
     1741 ggcgcccgat agaaggtccc gaccacgggg gagcgtaccg cgtgcagccc ctccatctcc
     1801 ggcgaagtct ctggctcgac catctcggga acgccctggg cgaccggcgc cgcctgctgg
     1861 gccacggggg cctctggctt ggccttgacc gagatctccg tctctccccg gcggaccagg
     1921 atctcaccga ccccgctctc ccgcaggagc tcgacgagcc gctcgacctc gccgagcggc
     1981 agattcggat tttcttcgat ctccggcata gtcagggatt ctacacgtct gttcgtggga
     2041 aaacccgcta ccagaagtac aatcggggat acatggccga aaaggagacc gggcgcgcga
     2101 gaataaaggt cctcgacccg agggtggccc agcaggtggc cgccggagag gtggtggacc
     2161 gcccggcctc cgtggtcaag gaactggtcg agaacgccct ggacgccggc gcctcccggg
     2221 tggaggtcga gctgggagac ggcggcacct cgcgtatcct ggtacgcgac aacggctctg
     2281 ggatgacccc cgagaacgcc aggctctgcg tgatcaggca cgcgacgagc aagatccgaa
     2341 acgtcgaaga catcgaggcc gtgatgacgc ttggcttcag gggtgaggca cttccgtcgg
     2401 tggcaagcgt ctcctccttc accctgacga cctcgacggg cgagggcgcg gccaccaggg
     2461 tcttcgtgga cggcggggcc gaggccgagg tgtcggccgc ctcccatccg aaggggacaa
     2521 ccgtgctggt ggaccgcctg ttctacaacg ttccggcccg ccgtgccttc ctgaagggcg
     2581 cgagggcgga gcgggctgcg atagtggagg tctccacgca cctggcgata gcccacccga
     2641 ccgtggcgtt ccgcctgacg gaggggggca gggactatct atcactcccg gaggctgggg
     2701 acctcctgga gcgcctggcc cagatcctgg gggttggcaa ggcgcgggcg ctgcgcaggg
     2761 tcgagtacga gtctgggcct ttcggcgtct cgggctacgc cgcccttcca agcctgacag
     2821 aggggagcag ggcccaccag acgatctcgg tgaacggccg cttcgtgagg gcggagggcc
     2881 ttcaccgcgg cctcgacgat gcctacaggg cgaccctgcc ctccggccgc tacccgccgg
     2941 tggctctgga gatcaccgcc gacccccgcc gggtggacgt gaacgttcat cccaccaaac
     3001 aggtggtccg cttctcggag gagcgggccg cccgcgaggc ggtagccgcc gcggtcaggg
     3061 gcgccatcga gtggcgcccc tcttccccac cctcggcccc gcgccccccc gaaagagcgt
     3121 ttacccagga ccgcttctcc ggatactcgc gaccacgaac cgattacccc tcccgcgccg
     3181 cggaggatcc ttcaccgtac gacgcacctc cggcgcggag cctgcaggag gcgcgcgaac
     3241 gcctctcgga ggcctcgcgg ccgcttgagc agccacccac cggggagcgg tacgacgcgg
     3301 aaccgtacac ggatcttccg gagcggggcg gtctaccccg gtcagccccc gaggaactgc
     3361 gcgttatcgg acagctcgcg gccggttaca tcctcatgga ggagccggaa tcgttgtggg
     3421 tggttgacca gcacgtggcg cacgagcggg ccatcctgga caggctgcac gactcggaga
     3481 gcccggccac ggtgcagagc ctcctggtcc ctgaggtcgt ggatctctcg cccgcggagg
     3541 ccgagtttgc cgcggcgagc ctggaggagc tctccgtcta cggcttcgag gccgagccgt
     3601 tcgggccccg gtccgtgcgg gtgacggcgg tgatctccac cctcgccgaa agggatgtag
     3661 ccggcgcctt caaggacgcc ctctcggcca tctcgggcac cgagccgggc caccgccgcg
     3721 aggaccacat cctcgccacg atagcctgcc actcggccgt aaagatgggc gacaggctct
     3781 cgccgcaaga gatggaggcc ctcgtaaaga gctggctgac cagccgactc cccgccacct
     3841 gcccgcacgg caggtccatc tgctaccgca tcggcctgaa cgagatagcc cgcaagctcg
     3901 accgccatta aagcgccctc cgtcccctcg ggcctgcgcc tcgcaggctg ccaaccatcg
     3961 gctttcagca acaaccaata agctgacagc tgatagctga tctgggtctc ggcccgcgaa
     4021 gggcgtgcgg tgctcgtccg ccggtacgct ggcctagtgt tcttcgcctt tgcggcggaa
     4081 ggagaggcgc cattcctcga tggccctggg tccccggtct gcgagttcga ccgtgtagac
     4141 gaccctgtag aggttccagt ccacgtggtg gcctataacc accccgggtt cgcgcgcggc
     4201 gccgcccctc agaacccata cccgatcgcc cttctcgtac cgggtctgtt gtgcctcgta
     4261 ggtgctgccc atcgcccgtt ctccaggtct actagagaca gggtaccacg cggaacatcg
     4321 agggcatccg gccgccggca ggcgcgcggt tcttgcccta taaataccgc tggcgagagc
     4381 aaggatgggg tttggatcct accagagacc ggatactcgg ggtgttggcc gtcgtaacgg
     4441 cggcctctat ctgggccacg cttgggttct ttgccaagat actctacgct cggggggtct
     4501 ccttcgaatc gctggtcgcg gtacgcgcat ctgtgggatg ggtggccgtg atgaccttcg
     4561 tcctcgccac gggtagggcc aggagcctgc gcgtctcccg cagggacctg gctttcctgg
     4621 cgccacttgg cctcttcggg atcggcgcct tctacctgtt ctacttctac acggtgaggg
     4681 agagcacggt cggaactgcg gccgtactga actacacctc gcccgctttc gtggtggtgc
     4741 tggcctacct gttcctccag gaggggctga acgcggccaa ggcggccgcg ctcctcctta
     4801 ccgcgggcgg catcttcctg gtggccggcg cctacgaccc gtccaacctt gaggtcaccc
     4861 cgacggtgct cctcacaggc ctgctctcgg gcctgaccta cggcctgtac tccatcttcg
     4921 gacgcccggt taccgggcac ctgagcccgg caaccatcct gagctacgcc ctcttcttcg
     4981 gctcctcgct gctcgtcgtg gccgcgctgc ccacgctcca cacgctggcc ggcctcccgg
     5041 cgagctccta cgccatgctc ttcatgctct cggtggtcca caccacgctc gctttcgccc
     5101 tctacacgtt cgggctcggg cgactcgggg ctggccgggc cgccatcgtc gccacggtgg
     5161 agcccgtcgt ggccgtcgcg ctcggaacgg cgctgctcgg cgaggccctg accgttccca
     5221 agctggtcgg cgcctcgctc gtggtccttg gcgcggcgct cgcccaggta aagctccgca
     5281 agcgccccac ccggaacgct gatagctgac ggccattggc tgaactaccg gcacaaagcg
     5341 ggtactatgt tggcccacgt caagagtcgt cgattcccgg ggattccctg ggccccggaa
     5401 aatggaggaa gaaggccatg cccatagagt tcaaggaagg caactcgggc tacaccctcg
     5461 gggtcgaaga ggagctctcc atagtcgacg ccacgaccgg agaactggtg cccaggatcg
     5521 aggagatcat gagccggcta ccagagcctt tgaaggaggc cgtctcctac gagttgttcc
     5581 agtcggtgct cgagatcaag acctccgtct gcgacacggt cgccgagaca gagaggcaat
     5641 tgcgtgagct gcgcggcagg gttggttcgt gggccgcagc ctgcggctcc tcgctcgcct
     5701 ccgcgggcac ccaccccttc tcccgctacc gcgaccagaa ggtcaccgac caggagcgct
     5761 acaagaacgt gataaggacg ctgcgctgga tcgcgcagcg ggaggtgatc ttcggccagc
     5821 acgttcacgt cgccgttccc ggcccggagg aggcgatcat tgcccacaac cgtctctcgg
     5881 agctggcgcc gctcttgctg gcgctctcga cgaactcacc tttctggcag gggttcgaca
     5941 caggcttcga gtcgtcgagg gtgaagatct tcgagggctt cccgcgcgcc ggcctccccc
     6001 ccgccttccc cgaatacgag gccttcgagg aatacgtgga cctgatggtg gccgcgggag
     6061 cgatggaaga ctacacctac tgctggtggg acgtgcgccc ccacccgggt atcgggacca
     6121 tagagttgcg ggtgctcgac tcccagacca gcctgaagta caccacctac cttaccgccc
     6181 ttaccaggtg cctcgtggcc gaggtcctcg gggaggtcgg cccgcacaag ccgtacaacc
     6241 cgcacctgac gctggagaac aagtggcgtg ccgccaggca cggcatggac gcggccttct
     6301 acgacgccga actggacagg acgatcccag ccagagacct agcccgcacc ctggtcgagc
     6361 gcctccaacc ccacgccaaa gacctcgact gcgaggacga tctggaaggc atcctcgaga
     6421 tcgtagaagg cggcacggga agccagcacc agcgcagggt ctacgaaaaa agcggcgact
     6481 tcctggacgt ggtcgccttc ctaatagaag gaacacgccc gacatagccc gctgcggcct
     6541 tcggcctacg ctcgtcagca cactacgccc tccaggctcc gtttgtcagc aacgcttcgc
     6601 ccggtttcgc ctagagctcg ctttcagcac gctcccttcg gtcgctttca gccgatcccg
     6661 ctgcggcctg cccccttccg ctttgaaggt tcgatactca ggaaagccct atgtcgaagc
     6721 taccgttgcg aatcgagacg accggccgaa agcgagagcc ttcaggctcg aagcgttgcc
     6781 gacaagcgga ggcgttagcc gcctctactc gaacccgatg ctccaggcgc ggtcgaggtc
     6841 gtagttggag taggcgcgga aggccaccat ggtctcggtg cgtgccacgc cggggacctg
     6901 ggcgatcttt tcggggacga tctcggcgag gctctcgaag tcggggatgc ggaccatgac
     6961 gaccaggtcg taggggccgg ttaccgagta ggcctctgtg accccttcta tgtcgagcat
     7021 ggcctgggcg gcctcctgga cacgctcgcg ctctgttctg actagtacta tggctgttac
     7081 cacgcgcggc accccctata tcccaaactt gaccgctccc gtgagcgtgt tgttggaggt
     7141 ggctttctcg tactccacgg ggcccacttt gatagagacc ctcgcggtct cgtcgagctc
     7201 gcccggaagg aagccgccga cctccaccgt ggtcgatttc ttggcgtcga ggcgcgatat
     7261 ggtctcgctc tgagcctggc gctcggcggc agtgttcatg atgacctcca cctcgatgcc
     7321 cgtctccgtc acctcaccgc cgttggtgac ccgcactgcg aaggtcggct cgtccgggcc
     7381 ggtcaggacc acctgaccgt cctgacggag tggttttccg gccacctgca cggacgtgac
     7441 ctgcaccccg tgcagggcgt tcgggtcgct ctcggccgtg cccgtcgtcg gcgcggcgtc
     7501 gaagcctagc gccccgtaat ccatgaacga ctgcggctcc tggaggtagg tttgatcccc
     7561 ggtctgtccc gccgtctcga gggagcgttt gacggccgga acgtagaagt cggcgatgat
     7621 ggtgtcgcta gtcctgtagt cggcaacggc cgtagcgagg tctttctcga acccttcctc
     7681 atctccggat gccgcctgag ccagggtgtt ggtggcctgg gatctta
//
LOCUS       DBNGVD010000392         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_823077, whole genome shotgun sequence.
ACCESSION   DBNGVD010000392 DBNGVD010000000
VERSION     DBNGVD010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_823077"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC16CM_10565"
     CDS             complement(<1..437)
                     /locus_tag="AC16CM_10565"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3140316.1"
                     /translation="MNHDELFTAGAEGHDPPPGRSERDSSRPAANPYADLKRIVKERG
                     LMDGQPAYFYGKFALNVCMLAVGLALLPILGSSWLQLANAVYLAVVFVQISFLAHDLG
                     HRQFTFRTPWKNNWATLIVGDLLLGMSRQWWIEKHNEHHAHPN"
     gene            644..805
                     /locus_tag="AC16CM_10570"
     CDS             644..805
                     /locus_tag="AC16CM_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140317.1"
                     /translation="MPGDRGEWWRCSEIGKMHVGPHGSSIGEPERRLMAVEHPKLREE
                     PEQGEIYYP"
     gene            833..2317
                     /locus_tag="AC16CM_10575"
     CDS             833..2317
                     /locus_tag="AC16CM_10575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140318.1"
                     /translation="MTAIVQETTSTGNTVARVVVAVDPELVPTVVDFARRVTERVGLG
                     AEAAGRLGRTLGEVCNNVIEYAFSPGEVRLYEVEVLRRAGRVVVAVEDMGLPFDYAHL
                     GEVGDQEVFGGSFVDEVRLLNLGRGGNRVELVKLLPQADIREDVGEEEHRKTIEAPEV
                     PDDASYEIRSMRPDESLQLARCIYRSYGYSYDWDDVYYPDRIRELQEGRLMRSCVAVA
                     PDGEFVGHLAVRVERPGAPVGESGQAVVDPRYRGHHLFPRMKTFMLKRAEREGMYGLY
                     GEATAVHPYSQRDSLKLGAGETGFLLGFIPGSVSYKEIGEDREGRRGSVALLYTRTNP
                     EPERTVYPPAAHRDTVRRIVEHNALRRTFGNGAGTEMQTSSRMTVEVNREHNLAFLRV
                     EEPGADLRELVRVRLHELCQQRVDCIYVDLPLSHPAVEEAGSGLGELGFFFGGVLPEL
                     RDGDVLRLQYLNEVDIRPEDVQTGSDFGRELADLVFRDKTSADD"
     regulatory      2474..2552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2601..>2943
                     /locus_tag="AC16CM_10580"
     CDS             2601..>2943
                     /locus_tag="AC16CM_10580"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3140319.1"
                     /translation="MADSERRTRGGPVLAAVVCALLATILVALVFPTRDARSAATVPD
                     GFAHSRVVGGLTNPTAMDFAPAGRLFVAEQRGVLRVLKADGTLTTFLDISGKVDAAGE
                     RGLLGVAFDPAF"
BASE COUNT          525 a          912 c         1007 g          499 t
ORIGIN      
        1 tggttcgggt gggcgtggtg ctcgttgtgc ttttctatcc accactggcg gctcatgccg
       61 agcagcaagt cgcccacgat gagggtcgcc cagttgttct tccagggggt gcggaaagtg
      121 aactgcctgt gcccgaggtc gtgggcaagg aagctgatct ggacgaaaac gacggccagg
      181 tagacggcgt tggcgagctg tagccagctg ctcccaagga tcggcagcag ggccaggccc
      241 accgcgagca tgcagacgtt cagggcgaac ttgccgtaga agtaggcggg ctggccgtcc
      301 atgagccctc gctccttgac gattcgcttc aagtcggcgt aggggttggc ggcggggcgg
      361 gaagagtccc tctcgctccg gccaggcggt ggatcgtgac cctcagcgcc ggcggtaaac
      421 aattcgtcat ggttcaagga acctggtcct ttcctgtagg cccggctgtt gtatggcagg
      481 gcggtgccgc actagtgtag ggcaacttcc ggcagattta tgctgttctt cttactatcc
      541 tccgatcgca tcgccggcta gagagaggat taccggttcg gggcacctat cgggtcacat
      601 ttccgcggcg ggagattcgt aactgcggcg tgccggcacg gagatgccgg gtgaccgcgg
      661 cgagtggtgg cgctgctcgg agataggtaa gatgcacgtg gggccacacg ggagcagcat
      721 aggggaaccg gagcgtaggc tcatggccgt agaacacccg aagttacgcg aggagccgga
      781 gcagggggag atctactacc cctgatcccg gacgcggagg aaggcaaccg cattgacggc
      841 gatcgtgcag gagacgacga gcaccgggaa caccgtagcc agggtggtgg tggcggtcga
      901 ccccgagctg gtgcccaccg tggttgactt cgcgcggagg gtgaccgaga gggtcgggct
      961 cggggccgag gcagcaggac gcctcggccg gacgttgggg gaggtctgca acaacgtcat
     1021 cgagtacgcg ttctcccccg gcgaggtgag gctgtacgag gtcgaggttc tccgccgggc
     1081 tggtagggtc gtcgttgccg tcgaggacat gggccttccc ttcgactacg cccaccttgg
     1141 cgaggttggg gatcaggagg tcttcggcgg ctcctttgtc gacgaggtgc gcctcctgaa
     1201 ccttggacga ggcggcaacc gcgtggagct cgtcaagctc ctgccccagg cggatatcag
     1261 ggaggacgtg ggcgaggagg agcaccgcaa gaccatcgag gcccccgagg tccccgacga
     1321 cgcaagctac gagatccggt cgatgcgtcc cgatgagtcg ctccagctcg cccgctgcat
     1381 ctaccgctct tacggttaca gctacgactg ggacgacgtc tactacccgg acaggatccg
     1441 cgagctgcag gagggccgtc tgatgcgctc gtgcgtggcg gtggcacctg acggcgagtt
     1501 cgtcgggcac ctggccgtga gggtggagcg tccaggcgca cccgtcgggg agtcggggca
     1561 ggcggtcgtc gatccccgct accgggggca ccaccttttc cccaggatga agacgttcat
     1621 gctgaagcgg gccgagcgcg aggggatgta tggcctctac ggcgaggcca cggccgttca
     1681 tccatacagc cagagggaca gcctgaagct gggggccggg gagaccgggt tcctgcttgg
     1741 cttcatccca ggctccgtct cctacaagga gataggcgag gacagggagg gccggcgcgg
     1801 atcggtcgcc ctcctctaca cgcgtacgaa ccccgagccg gaacggaccg tctacccgcc
     1861 cgcggcacac cgggatacgg tcaggcgaat cgtcgaacac aacgcgctac gccgcacctt
     1921 cggcaacggt gcgggaaccg agatgcagac ctcatcacgg atgaccgtag aagtgaacag
     1981 ggaacacaac ctcgccttcc tgcgcgtcga ggagccgggg gccgaccttc gagagctcgt
     2041 gcgggttcgg ctacacgagc tctgccagca acgtgtcgat tgcatctacg tcgacctgcc
     2101 actctcccac cccgccgtgg aagaggctgg atcagggctt ggtgaactcg ggttcttctt
     2161 cggcggggtc ctgcccgagt tgcgcgacgg cgacgtgctt cggctccagt acctgaacga
     2221 ggttgacatc aggcccgaag acgtgcagac cggatccgat ttcggccggg aactcgcaga
     2281 cctcgtcttc agggacaaaa catcagccga tgactagcca aacctctccc ctaggcaacg
     2341 aagaacggtc tccgaacacc tatgtgtaac gtcccatccg cgtgtccaga cgaaccgacc
     2401 ctccccgaaa agccccgtca gcgtccgaac atcggcatct cctggatgaa agctaatata
     2461 tactggcaac cgttccggac aaaggcaaac ccgccgtgag gcagggacgc aaagccacag
     2521 gcctcgtttg ccgaggtagc caggccgccg aaaggagacc gttcatgatt ccgccccgct
     2581 cctcttgcgc tagcttgcgc atggctgatt ccgaaaggcg aacacgcggc gggccggtgc
     2641 ttgccgcggt ggtctgtgcc ttgttggcca ccatcctggt agccctggtc ttccccacgc
     2701 gcgacgcgcg cagcgccgcg accgtcccgg acggtttcgc acactcgcgg gtcgtcggcg
     2761 gcctcacgaa ccccacggcg atggactttg cgccggccgg ccggctcttc gtggccgaac
     2821 agcgcggcgt gcttcgggtg ctgaaggcgg acgggaccct gaccacgttc ctggacatct
     2881 cgggcaaggt ggacgcggcg ggcgagcgcg gcctgctggg cgtcgccttc gaccccgcct
     2941 tcc
//
LOCUS       DBNGVD010000393         8757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4830163, whole genome shotgun sequence.
ACCESSION   DBNGVD010000393 DBNGVD010000000
VERSION     DBNGVD010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4830163"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16CM_10585"
     CDS             <1..263
                     /locus_tag="AC16CM_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3140320.1"
                     /translation="TVGQARGVALLAAYRAGIEVTEYTPLQVKQAVTSYGRADKRQVQ
                     EMVKALLNLPAVPRPDDAADGLAIAICHAFSSNLPGKVGVGK"
     gene            288..878
                     /gene="ruvA"
                     /locus_tag="AC16CM_10590"
     CDS             288..878
                     /gene="ruvA"
                     /locus_tag="AC16CM_10590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3140321.1"
                     /translation="MIYRLRGTLVEKDTEGVVVDVGGVGYRSSASLATLRALPSLGEE
                     CVIHTRLVVREDAMLLFGFAEREERAAFDALTAVSKVGPKLAISVLSSMSPREVSEAV
                     SRGDVIKLSSVPGLGKKTAERLVLELRGKSIASSGSDPVVATGGGGGPYMEARDALAA
                     LGYRLEEAEKALNDVPPQDSVEKYIKEALRRIGSRR"
     gene            875..1966
                     /gene="ruvB"
                     /locus_tag="AC16CM_10595"
     CDS             875..1966
                     /gene="ruvB"
                     /locus_tag="AC16CM_10595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3140322.1"
                     /translation="MSAGEMDDFTVRSEQRDEPYEDITGASGFADFGEHPLDPEAHVG
                     DDEPTLRPSSFDEFVGQESLKQNLKIFVTAAKQRGESLDHILLDGPPGLGKTTLCRIL
                     AEEMGVGLRPTSGPTLEKAGDMAAILTALEPGDFLFIDEIHRLNRQIEEVLYPAMEDY
                     AMDVVLGQGPSARTIRIDLPPFTLVGATTRKGLLTKPLLDRFGVAERLEYYGPEDLQK
                     IVTRNAGILNVPITDGGASQLARRSRGTPRIVNRLLKRVRDYAQVVANGEIDEQTANA
                     ALDMQGVDQLGLDRADRDYLGLVVDKFDGGPVGVGTISVALGEARDTVEDVYEPYLLQ
                     SGLIQRTPRGRIATNRAYRHLGVPVPSNG"
     gene            2039..2236
                     /locus_tag="AC16CM_10600"
     CDS             2039..2236
                     /locus_tag="AC16CM_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140323.1"
                     /translation="MRYDVHPPGLSEPGGVTEEDFGFAAFSLAHAACGEDVLEVRLGE
                     TALLEWCTSCDAMKIFVPSGA"
     gene            2436..3536
                     /locus_tag="AC16CM_10605"
     CDS             2436..3536
                     /locus_tag="AC16CM_10605"
                     /inference="COORDINATES: protein motif:HMM:NF021466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HIR3140324.1"
                     /translation="MTRLTRTALLVARSVEERFDQFRFDFKRRFGLLDPFEILPYRGH
                     GTSRELFLRGRVLEETGITRSGRNDTIWRNVLNMVRRFASDEVAGARVLATFDGLRAE
                     TVTDEEGFFEVRFRLDEPLDGSSRWHPVDIELLSPPSPGGGEVRSKGQVLVPHGAAFG
                     VISDLDDTVVRSSATNLLKMAWVVVRNNAHTRLPFEGVGAFYEALRRGEGGDAFNPIF
                     YVSSSPWNIYDLLEDFLNVHGIPPGPLFLKDWSPTVLGKHQDYKLGVIRTLLATYEDL
                     PFVLLGDSGEEDPEIYLQVIREHPGRIMTAYIRDVSPDKRDAEVQAIASEARRLGTEM
                     VTVPETVAAAEHAVSAGLISPDSLARVRSRSD"
     gene            complement(3550..4050)
                     /locus_tag="AC16CM_10610"
     CDS             complement(3550..4050)
                     /locus_tag="AC16CM_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3140325.1"
                     /translation="MPPDKNPFLIAPDDGVRLPVLDITHKVTAAASGGSLVVDEWCLA
                     PGQTIPPHTHLREDECSYVLEGELTCYVGGEVVRANAGSYIVKPRRVPHAFHNAGART
                     VRVMEILTPGWSFEGYFDRYEEIASRSTSVGDHETARAELSKGYDITWHDDMVSEVKA
                     RFGIGP"
     gene            complement(4319..5059)
                     /locus_tag="AC16CM_10615"
     CDS             complement(4319..5059)
                     /locus_tag="AC16CM_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140326.1"
                     /translation="MMRTIFDKVMWVGRATVFVVGSTVILALTVGIASAALVGTGVGA
                     TLDLGRANSVNAVTRLVGNVPAASLQIDNNSSGTRATALTLRVEPGTAPMKVDSGTKV
                     TNLNSDRVDGKDSSAFAVGTNGKAKDAFNADFASFAGQAQVAQVSTDASNLDGKDSTD
                     FLPVDTYTDTADAVTGQGGGTMLVFTAIPCDPGDKILNAGGGANQEDDMIEERPTGAN
                     SWLVRIRDNGSPTTSTAVANCLDFPPRH"
     gene            complement(5131..5730)
                     /locus_tag="AC16CM_10620"
     CDS             complement(5131..5730)
                     /locus_tag="AC16CM_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3140327.1"
                     /translation="MSRAGDVFENPITRERAVVRLGTEESDGELIVADLYVGPGGRVA
                     AEHVHPILTEWFTVLSGRVGFRLDGQEYVAKIGERLCVKPGTVHDWWNAGEEEAHVVV
                     EVSPAARFEEAISNTFGLARDGKTNARGMPNLLQLALLSREFEDVLYFTSPPRVLQRV
                     LFAVLAPIARLLGYRGSYPEYATPLAPSRQRPVQGVSRR"
     gene            complement(5841..6500)
                     /locus_tag="AC16CM_10625"
     CDS             complement(5841..6500)
                     /locus_tag="AC16CM_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140328.1"
                     /translation="MRSTRMMKAVLDGAWRLGRGTATVMGLAVLLALTVGLASTALAG
                     TGIGARFDLGKINTVNAVSKLAGNVAGPSLTIDNNSTATGATALDLQVEPGKAPFKVN
                     STTEVQGLNVDSIDGKNSSDFLQEASDRDDFLPNDTYANDAFKVGPGSGGTVFASVDC
                     DPGDKLLGGGGAALDPAQDTTTASVPFVAGQGWDYQIRDNGSPSSVEAFVVCADFPPE
                     H"
     gene            complement(6790..7227)
                     /locus_tag="AC16CM_10630"
     CDS             complement(6790..7227)
                     /locus_tag="AC16CM_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140329.1"
                     /translation="MNDTNELRSLDLGEMDEREAQRFLTELLSKLDPEEEYNVRHPDD
                     YMLEMPQSGERIRGRERMRRFQQSYAALSPSDPAHGIRVNRVLVRDGLWVVEGLAEYD
                     EGRTDLNVVLILELRDGMMWRDRWYFAEPFEAPEWRSQWTEHP"
     gene            complement(7239..7802)
                     /locus_tag="AC16CM_10635"
     CDS             complement(7239..7802)
                     /locus_tag="AC16CM_10635"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR3140330.1"
                     /translation="MTIKVTTDTGELQEIAVLPTESALPRAARFLSDLVKDEGFVRGE
                     ILPILEAAKDEEDWYVARQYEAEDGSYVLKVFVWPRGTGTMIHDHSSWGAYSCVLGTV
                     LEERYDRLDDGSVFEHARLNKAWQLTWSRGDGASTVLPGNGGIHRVGNPGESLAISVH
                     LYGPRLEDIDGRDYDPSRDYVCDRLED"
     gene            complement(8117..8692)
                     /locus_tag="AC16CM_10640"
     CDS             complement(8117..8692)
                     /locus_tag="AC16CM_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009816895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140331.1"
                     /translation="MSVSNRRTAVGTLAIGQHPEGETVPGAWFHPEVRIGHVHLRVAY
                     LARATAFYRDILGFDVMAYGPDFGLPGAAFLSSRGYHHHLGLNTWESEGGTPPPAGHS
                     GLYHFTLLYPGRAELGRALRHILGREYPIDSAEDHGATVSVYLRDPDGNGIELYYDRP
                     RSEWFDPQGGPILRADPFDVCELHSEADNAG"
BASE COUNT         1541 a         2693 c         2822 g         1701 t
ORIGIN      
        1 tcacggttgg gcaggcgcgt ggcgtcgcgc tgctggccgc ctacagggcc ggcatcgagg
       61 ttacggagta cacgccgctc caggtgaaac aggcggtcac ctcctacgga agggcggaca
      121 agcgacaggt ccaggagatg gtcaaggcgc tcttgaatct acccgcagta ccgcgtccag
      181 acgatgcggc tgacggcctt gccatagcca tctgccacgc cttttcgagc aatctgcccg
      241 gcaaggttgg ggtggggaaa tagccactca tgcgcgataa ttagcccatg atttacaggt
      301 tacgcggtac tctggtcgag aaggatactg aaggcgtggt cgtggacgtg gggggcgtgg
      361 gctaccggtc ttcggcctca ctcgcgacgc taagggccct gccctcgctc ggtgaggagt
      421 gtgtgatcca caccaggctg gtggtgcgcg aggacgcgat gctcctgttc gggttcgccg
      481 agcgggagga gcgggcggcg ttcgacgcgc ttaccgccgt tagcaaagtc ggtccaaagc
      541 ttgcgatctc cgtgctctct tccatgtctc cacgggaggt ctccgaagcc gtgtctcggg
      601 gggatgtgat caagctctcc tccgtgcccg gtctcggaaa gaagacggcg gagcggctgg
      661 tgctggagct gaggggcaag agcatagcgt cctctgggtc ggacccggtg gtcgccaccg
      721 gtggtggcgg aggaccctac atggaggcga gggatgcgct cgcggcactc gggtacaggc
      781 tcgaggaggc tgagaaggcg ctgaacgatg tgccgccgca ggattccgtc gagaagtaca
      841 taaaggaggc gttacggcgg atagggagca ggcgttgagc gcgggggaga tggacgattt
      901 caccgttaga agcgagcagc gggacgagcc ttacgaggac ataacggggg cctccggttt
      961 cgcggacttt ggggagcatc cgctggaccc cgaggcccat gtgggggacg atgagcccac
     1021 gctcaggcca agttccttcg atgagttcgt cgggcaggag tcgctcaagc agaacctcaa
     1081 gatcttcgtc accgcggcca agcagcgggg ggagtcgctc gaccacatac tgctggatgg
     1141 acctcctggt ctcggcaaga ccaccctgtg ccgcatcctc gccgaggaga tgggcgtcgg
     1201 cctcagaccg acgagtggtc ccacattgga gaaggccggg gacatggcgg ccatcctgac
     1261 ggcgctcgag cctggcgact tcctgttcat agacgagatc caccgcctca acaggcagat
     1321 cgaggaggtc ctctacccgg cgatggagga ctacgcgatg gacgtggtgc tcggccaggg
     1381 tccgtcggcc cgcaccatca ggatagacct gccgcccttc acgctcgttg gtgcgacgac
     1441 caggaagggc ttgcttacca agccgttgct cgaccgcttc ggcgtcgccg agaggctgga
     1501 gtactacggg ccggaggacc tgcaaaagat cgtgacccgc aacgcgggca tactcaacgt
     1561 gcccatcacg gatggcggcg cgagccagct cgcccgccgc agtcgcggaa cgccgcggat
     1621 cgtcaacagg ctcctcaagc gggtacgcga ctatgcccag gtggtggcaa acggtgagat
     1681 cgacgagcaa acggccaacg ccgctctcga catgcagggc gttgaccagc tcgggctcga
     1741 ccgggccgat agggattacc tcgggctggt cgtagacaag ttcgacggtg gcccggtcgg
     1801 cgttgggacc atctccgtgg ccctcgggga ggcgcgcgat accgtcgagg atgtctacga
     1861 accttatctg ttgcagagcg gcctcatcca acgcacaccg cgcggaagga tcgcaaccaa
     1921 ccgggcctac cggcaccttg gcgtgcccgt tccgagcaac gggtagggtc gccccggtgc
     1981 agggcttcac cccacaccgc taatgttcaa tgaggatccg gtcgatacca ggagcgtaat
     2041 gcgctacgac gtacatccgc caggcctttc ggagccagga ggcgtgaccg aggaagattt
     2101 cggcttcgcc gccttcagcc tggcgcacgc agcttgtgga gaggacgttc tcgaggtgcg
     2161 cctcggtgag acggcgctgc ttgagtggtg tacctcgtgc gacgcaatga aaatcttcgt
     2221 tccgtcgggg gcctgatcca gccggttcca ggcggcccct ggcggtggtt gcaagtcccc
     2281 aggggatcta acatgatggt tacgctcttg ctgaggggag gagatgttcc ttgcaggcgg
     2341 cttgctcgtg gtgttagctt ggggcgtctc gcataaggaa attccttttg gcggtaagga
     2401 ggacttcggg gaatgatggg gcgcgtggaa cgttgttgac aaggcttacg agaacggcgc
     2461 tgctcgttgc ccgctctgtg gaggagcgct tcgaccagtt caggttcgac ttcaagaggc
     2521 gtttcgggct gctagatccc ttcgagatcc taccctacag ggggcatggc acgagtcggg
     2581 aattgtttct gaggggccgc gtcctggagg agaccgggat cacccgttcc ggccggaatg
     2641 ataccatctg gcgcaacgta ctcaacatgg tcaggcgctt cgcgagcgac gaggttgctg
     2701 gcgcgagggt gctggccacc ttcgacgggc ttcgggcgga gactgtgacc gacgaggagg
     2761 ggttcttcga ggtacgcttc cggctcgacg aacccctcga tggcagctcg cggtggcatc
     2821 cggtggatat cgagctgctc tcgccgccat ctccaggtgg gggggaggtg cgctcgaagg
     2881 ggcaggttct cgtaccgcat ggggctgctt tcggtgtgat cagcgacctg gacgataccg
     2941 tggtccgctc cagcgctacc aacctgctca agatggcctg ggtcgtggtg cgcaacaacg
     3001 cccacacgcg gcttcctttc gagggcgtgg gtgcattcta cgaggcgctt cggcgagggg
     3061 agggaggcga tgccttcaac cccattttct acgtctcgag cagcccttgg aacatatacg
     3121 acctgctcga agatttcctg aacgtgcacg gcatcccgcc cggcccgctc ttcctcaaag
     3181 actggagccc gaccgtgctg ggcaagcatc aggactacaa gttgggcgtg atacgcacac
     3241 ttcttgcgac gtacgaggat ttgcccttcg tcctgctcgg ggactccgga gaggaggacc
     3301 cggagattta cctccaggtg atacgcgagc acccggggcg gataatgacg gcctacataa
     3361 gggacgttag cccggacaaa cgcgatgccg aggtgcaggc tatcgccagc gaggcgcgcc
     3421 ggctcggtac ggaaatggta accgtacccg aaacggtcgc cgcggccgag cacgccgttt
     3481 cggccggcct tatctctccg gactcgctag ccagggtccg ttcccggagc gattagcact
     3541 agtaccgcgt caggggccga tcccgaagcg cgccttcacc tcggagacca tgtcgtcgtg
     3601 ccaggtaata tcgtatccct tgctcaactc ggcccgcgca gtctcgtggt ccccgacgct
     3661 cgtgcttcgc gaagctatct cctcataacg atcgaagtag ccctcgaaag accagccggg
     3721 cgttaggatc tccatgaccc gcaccgtcct ggcgcccgcg ttgtggaagg catgaggaac
     3781 ccggcggggc tttacgatgt aggagccggc gttggcccgc acgacttccc ccccaacgta
     3841 gcacgtcagt tcgccttcga ggacgtacga gcactcgtct tcgcgtaggt gggtgtgggg
     3901 tgggatggtc tggccgggag cgaggcacca ttcatcgacc acaagcgaac cgcccgaggc
     3961 cgcggccgtg accttgtgtg ttatgtcaag gacgggcagg cggaccccat cgtcgggggc
     4021 aattaagaat gggttcttat cagggggcac gacgcgacct cctccggggt acctccgcaa
     4081 ggtggcgggt tggatgtgga agcggcgccc tgtcctgagt cgccttcctc ttctggtatt
     4141 gcaggcgcgc tacctcggcc tcctgtaagg ccatcatgta gtagctcaac ccgtacatgt
     4201 tcgacgctcc tctctttatt cagcaggcag acccgtccgg gacgctacct cgggggttgc
     4261 cggtgggagt gatactggac gtgggggaga tagtcgccat ctgcctgttt gtcctccctc
     4321 aatgccgtgg cggaaaatcc aggcagtttg ccacagctgt gctggtggtc ggcgacccgt
     4381 tgtccctgat acgcaccagc caactgttgg cacccgtagg gcgttcctct atcatatcgt
     4441 cctcctggtt ggcgccaccg ccggcgttga ggatcttgtc tcccggatcg cagggaatag
     4501 cggtgaagac cagcatcgta cccccgccct gtcccgtcac tgcatccgct gtgtcggtgt
     4561 aggtatcgac gggcaggaag tccgtcgaat ctttgccgtc gaggttgctg gcgtcggtag
     4621 aaacctgcgc gacctgggcc tgaccggcga aagaagcgaa gtcggcgttg aaggcgtcct
     4681 tggctttgcc gttggtgccg acggcgaagg cagaagagtc cttgccgtcc acccgatcgg
     4741 agttgaggtt ggtaaccttc gtcccggagt cgaccttcat cggcgccgta ccaggctcga
     4801 cccgaagggt cagtgccgtg gcgcgggtcc ctgacgagtt gttgtcgatc tggagcgagg
     4861 ctgcgggcac gttgccgacc agtctagtta ccgcgtttac cgagtttgcc cggccaaggt
     4921 cgagtgtggc acccacgccg gttcccacca aggcggcgga agcaatcccc accgtgaggg
     4981 ccaggatcac cgtcgatccc acgacgaaca ccgtggccct tcctacccac atcaccttat
     5041 cgaagatggt tcgcatcatc tgtccttcct ctttcccgtc gcgggcgatg agtttgcggt
     5101 gcgtcgaccg cggccagttc accggccggc ctaacgcctc gatactccct gaacggggcg
     5161 ttgcctcgaa ggagcgagag gggtcgcgta ctccgggtag ctgcccctgt agcccagcag
     5221 acgggctatt ggtgccagca cagcaaagag caccctctgg aggacgcgcg ggggactggt
     5281 gaagtacaac acgtcctcga actcccgcga cagaagggcc agctgcaaga ggttgggcat
     5341 ccccctggcg tttgtcttgc catcccttgc cagaccgaac gtgttggaga tggcctcctc
     5401 gaaccgtgca gcggggctga cctcgacgac gacgtgcgcc tcctcttctc ccgcgttcca
     5461 ccaatcgtgc actgtgccgg gcttcacgca caggcgttca cctatcttcg cgacgtattc
     5521 ctgaccgtcg aggcgaaagc cgaccctgcc gctcagaacg gtgaaccatt ctgtaaggat
     5581 ggggtgaaca tgttctgccg caaccctccc gccgggaccc acgtagagat cggcgacgat
     5641 caactcaccg tctgattctt ccgttccgag gcggaccacc gcccgctcgc gcgtgattgg
     5701 gttctcgaat acgtctcctg ctctgctcat gacttcgcct cttgctctga tctcgcgaat
     5761 tcgatctttc ggccgtgatt cctccgagcc cgagatcggg cggggcactg gaccccgccc
     5821 gtgtgggagg ccccgggacc tcagtgctcc ggtgggaagt cggcgcagac cacgaacgcc
     5881 tccacggaac tgggcgagcc gttgtcccgg atctgataat cccagccctg cccggccacg
     5941 aacggtacgc tggcggtcgt ggtgtcctga gccgggtcga gggcagcacc gccgccaccc
     6001 agaagcttgt cacccgggtc acaatctaca gaggcgaaca cggtgccacc tgatcctggc
     6061 ccgaccttga acgcgtcgtt cgcgtaggtg tcgttgggca agaagtcgtc cctgtcagac
     6121 gcttcttgca ggaagtcgct tgagttcttg ccgtctatcg agtccacgtt gaggccctgt
     6181 acctcggtgg tggagttgac cttgaacggc gcctttcccg gttccacctg gagatccaac
     6241 gcggtcgccc ctgtggcggt ggaattgttg tcgatggtga gcgaggggcc ggccacgttt
     6301 cctgccagct tgctcacggc gttgacagta ttgatctttc ccaggtcgaa gcgggctcct
     6361 atgcccgttc cggcaagtgc cgtcgaggct aaccccacgg tgagggcgag caagacggcc
     6421 aggcccatga ccgtggccgt gccgcgaccg agcctccagg cgccatccag aactgctttc
     6481 atcattctcg tcgatctcat ctcttgcctt tctctagtcg ctgttgtgga tgatttgcga
     6541 atggtccgac gtccgtgtct gccgtttcgt tctacatgag agcctcctct cggtgggtta
     6601 aggggcgcct cgcatctggg ctccgatttg gaccggatcg gcccgcgccc cacacggtgg
     6661 gttcggtgct ctctacaggt aaaccttatg cggaggggcc ccggttgcct atggcagaaa
     6721 catgccattt cgcctggggt tggggtgacg gtcgaggttg ccaccatgaa gaaatacccc
     6781 taaagagggc tacggatgct cggtccactg tgatctccac tcgggcgctt cgaaaggctc
     6841 tgcgaagtac cagcggtccc gccacatcat tccgtcacgc agttccagga tcagcaccac
     6901 gttcaggtcg gtcctccctt cgtcatactc ggcgagcccc tcgacgaccc acaggccatc
     6961 tcgtaccagc acacgattca ccctgatccc atgcgccggg tcggagggcg agagggcggc
     7021 gtaggattgc tggaaccgcc gcatcctctc cctgccgcgg atacgctcgc cggattgggg
     7081 catctcgagc atgtagtcat ccgggtggcg cacgttgtat tcctcttccg gatcgagttt
     7141 ggacagcagt tccgtgagaa atcgctgcgc ctcccgctca tccatctccc cgaggtcaag
     7201 cgaccgcagc tcgttcgtgt cgttcatggc tctcctactt aatcttctag ccggtcgcag
     7261 acgtagtcac gggaggggtc gtagtcacgg ccgtcgatgt cttcgaggcg ggggccgtag
     7321 aggtggaccg agattgccag gctctcgccg gggttgccca ccctgtggat accaccgttg
     7381 cccggaagca ccgtagaggc gccgtccccc cggctccacg tcaactgcca ggccttattc
     7441 aggcgggcgt gctcgaacac cgagccgtca tcgaggcggt cgtagcgttc ctcaaggacc
     7501 gtgcccagga cgcaggagta ggcaccccag gaggagtggt cgtggatcat ggtccccgtg
     7561 ccccggggcc agacgaagac cttcagcacg taggaaccgt cctctgcttc gtattgccgg
     7621 gcgacgtacc aatcctcctc gtccttcgct gcctcgagga tgggaaggat ctcacccctc
     7681 acgaatcctt catccttcac caggtcggat aggaagcggg cggcccgcgg cagggccgac
     7741 tccgtgggga gcacggcgat ctcctgcagt tccccggtgt ccgttgtgac cttgatagtc
     7801 atggggttct ccttctcttc agtggcgccc gtgggcattt cgtcttgccc ttcatcgtgc
     7861 tacctgcgcg accgttctcc cagcgacgaa tcgaaacaaa ggcccaaaca atcgtccgaa
     7921 ccgaacttgt ttgttgtcgg gtggcgggag caaccggata taccagagcc cgaacgaaac
     7981 gccgcacagc aaggggctcg gactccagtc cgagccctga cggccctagt tgctgccccg
     8041 gagtgaacgg gcgggttctg gggaacctgg cggttcgcgt tacggtgaaa gcccgttcac
     8101 cacggaggtg agaagattat cctgcgttgt ctgcctcaga gtggagttca cacacgtcga
     8161 acgggtcagc cctgagaatt ggcccgccct gggggtcgaa ccactcggat ctcggacgat
     8221 cgtagtacag ttcgatgccg ttgccgtcgg gatctctgag gtaaacggag acggtagccc
     8281 cgtggtcctc ggcggagtcg ataggatatt cgcggcccag gatatgccgc agggctcgcc
     8341 ccagttctgc acgaccgggg tagaggaggg tgaagtggta gagcccggaa tgccccgcgg
     8401 ggggtggtgt tccaccctcg ctctcccagg tgttcaagcc caggtggtga tgatagcccc
     8461 tcgacgagag aaaggccgcc ccaggcaggc cgaagtcggg gccgtacgcc attacgtcga
     8521 aaccaagaat atccctgtag aaggcggtag cacgggcgag gtaggcgaca cgcaggtgga
     8581 cgtgcccgat gcgcacttcc ggatggaacc aggccccggg cactgtctcc ccttcaggat
     8641 gttgtcctat cgccagtgtg ccaacggcgg tcctgcgatt cgagacgctc acctctcaat
     8701 cctttcgggc ggcagcatct ccatgtagcg accgatgatc tccatcatcc gctgcac
//
LOCUS       DBNGVD010000394         5560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5013457, whole genome shotgun sequence.
ACCESSION   DBNGVD010000394 DBNGVD010000000
VERSION     DBNGVD010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5013457"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="AC16CM_10645"
     CDS             complement(<1..849)
                     /locus_tag="AC16CM_10645"
                     /inference="COORDINATES: protein motif:HMM:NF021098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HIR3140332.1"
                     /translation="MTEYEHRSDRFTAVEDQYAGYTVVDESRTKIGKVDDLFVDENDQ
                     PEYFGVNMGFLGTSSTLIPADIATTKDEQGFIEISQPKSTVQDGPAFDDDREITPDYE
                     NEVRSYYGLGPVESSGSYEETNGRSGAGTTDSTTAGTVGSGMTMGDTESGEFREHDRD
                     QEGLSQPGSDLEDEDELRVQRSEEELVAGTREREAGSLKVRKRVRTDREQIEVPTRRE
                     EVSVERVPVSGEATEAQIGEDEVSVPVVEEEVVVQKKPVAKEEIRVRKDVVHERQTVE
                     EDVRREE"
     gene            1373..2467
                     /locus_tag="AC16CM_10650"
     CDS             1373..2467
                     /locus_tag="AC16CM_10650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3140333.1"
                     /translation="MEDTERSFEEHPEAARILDERFWEIFEDYKRRLREIKSLLVVGD
                     GTTPEQLEANARSVLGRAAKVLRGEESSLLAVEEEIYRNIESSYEPLNPHPDESFRAG
                     VALCKAAVTVVVKNLVPGTSPERIAGVALAVQEIVMDRVSRMVMASYVDYLLTKVRET
                     QFEERRRFSRELHDRLAHDMTVVIQSLELYRAYKESAPERAERRLVLARESADEALDL
                     MREFSKELRLLETSDGLRIALENLMRISVPEGVHSEVSLEGEERHLPDYVRDQLYMVL
                     REGVRNAVAHSGSERIEVEIAITPRHVTARVRDHGTGLDPEDGTTEGLGLSSMQERVS
                     LLGGSFELIPEPGGGARADVSLPLMREKGR"
     gene            2500..3150
                     /locus_tag="AC16CM_10655"
     CDS             2500..3150
                     /locus_tag="AC16CM_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3140334.1"
                     /translation="MLLADDHALVRQGMAEMLSTDPGMEVVAQAANGREAVDLAREQR
                     PDVVVLDVEMPVMSGQVALRRLLDLRPPPKVVIVTVFADESHVRELLALGAGAYLSKN
                     SSMRDLISTVRSVAPGGWEGQNEDDVVLFVPRTAFEENDPEESSLSEREAEVLMLAAR
                     ALSNREIAASLYLSETTVKRHLSNVYGKLGVRTRAEAARKALSEGWISAWDVSREV"
     gene            3352..3696
                     /locus_tag="AC16CM_10660"
     CDS             3352..3696
                     /locus_tag="AC16CM_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140335.1"
                     /translation="MRILIANTPLMYRETLALAVHRHNPGFEVMMANPASMDGEAKRF
                     NPHVLIRDDDGIEVASPDGVVCWVGIIIDDHLKARISLDGKVSEIHEVSLYELLAALD
                     EVTEFVSDNSIR"
     gene            complement(3732..4397)
                     /locus_tag="AC16CM_10665"
     CDS             complement(3732..4397)
                     /locus_tag="AC16CM_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3140336.1"
                     /translation="MPDDPIKVLLADDHTMFREGLAGILASYGGMEVVGSAPNGEEAV
                     ALAERTRPDVVLMQVQLPFEKARTLLTRMRAVLPPPKVVIVTMFESPRYLRELMSLGA
                     SAYLVKSASTQHLIGAVRAAVFDPKGENVVVGMPREMLEEARKGSGGVLSARELEILL
                     LAARGLSNREISENLHIAEATVRRHLANTYAKMRVSSRGEATRKALQEDWITVSDVTE
                     DTE"
     gene            4760..5152
                     /locus_tag="AC16CM_10670"
     CDS             4760..5152
                     /locus_tag="AC16CM_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140337.1"
                     /translation="MIDNAIEGRTEARSRFESSWLIVRDVIGAGGGLPEILTLETGGK
                     AKVNGRILPVFSFEEEARLFLHLCGLGGWWHATKSGAAELALVLSDDCSDVGRVALDP
                     IPEIGLCGPHDLVSLPRKEFVRLLAAGR"
     gene            5241..>5560
                     /locus_tag="AC16CM_10675"
     CDS             5241..>5560
                     /locus_tag="AC16CM_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR3140338.1"
                     /translation="MTDTKAGKEASHREGTGEQEQQLGVVFWVTVAISALFVLWGVLF
                     TENFASATATVVGWITEGLGWLYMLITTFFLAFVIYLAFSRYGKIKLGKPDEEPEFGY
                     FPWF"
BASE COUNT          959 a         1766 c         1791 g         1044 t
ORIGIN      
        1 ctcttcgcgc ctcacgtcct cctcgactgt ctgccgctcg tgcaccacgt ctttgcggac
       61 ccgtatctcc tctttggcca ccggcttttt ctgcaccacg acctcttcct cgaccacagg
      121 cacggacacc tcgtcctcgc caatctgggc ctcggtcgcc tcgccgctta ccggcacccg
      181 ctcgacgctt acctcctccc gacgggtggg cacctctatc tgctcgcggt cggtcctcac
      241 gcgctttctg accttcaggc tgccggcctc gcgctcgcgg gtgccggcga ctagctcctc
      301 ctcgctcctc tgcaccctga gctcgtcctc gtcttcgagg tcgctacccg gctggcttag
      361 gccctcctgg tcgcggtcgt gctcgcggaa ctcgcccgac tcggtgtcgc ccatggtcat
      421 cccagaacca accgtgccgg cggtggtgga atccgtcgtg ccggcgccag aacgtccgtt
      481 cgtctcctcg taggagccgg agctctcgac gggtccgagc ccataataag agcggacctc
      541 gttctcgtag tcgggggtga tctcacggtc gtcgtcgaag gccggcccgt cctgtacggt
      601 gctcttgggc tgggagatct cgatgaaacc ctgctcgtct ttcgtggtgg cgatgtcggc
      661 cgggatcagg gtggaactgg tgcccaggaa gcccatgttc acgccgaagt actccggctg
      721 gtcgttctcg tctacgaaca ggtcgtctac cttgccgatc ttcgtcctgc tctcgtccac
      781 cacggtgtag ccagcgtact ggtcctcgac cgccgtaaac ctatcgcttc tgtgctcata
      841 ctcggtcatc tactctccct cattcgtctt gtctactctg cgttgcgtgc atccgccgct
      901 ccctgcccat tccctacggc ttgtgatgcg ctgatcccct cgcataagac ccctgctaga
      961 ggcctggcgc ccgaggggtg gcaatgtgga aaggagaagc ggacgccagt tcgcactaca
     1021 gcccctaccc ctcttccggc caccgtgaac gcggattccg gccgttttcg gagaagtcgg
     1081 tacggaaggt gcaggctttt ggtccgaatg gtgtagcggc atgtgaaact ctcgtaaacg
     1141 ggcccagaat cctcgcgctt gcatgcctct ctctcgcgcg ccggtgagcc gattcaagtg
     1201 gtcccaagcg gtccgcgggt tggcgtcctt gatgctctgg tccgtgccag taaaggcgtg
     1261 ggcgagcgac tccgggggct cggggaccca gaaggcgcat ggtatcctgc gaaatgaaca
     1321 agagggtgac gtgagcctct agacaaagtg tttgggacaa acgaggatag ctttggaaga
     1381 cacggagaga agtttcgagg agcatccgga ggcggcccgg atcctcgatg agcgcttctg
     1441 ggagatcttc gaggactaca agcgccggct gcgcgagata aaaagcctgc tggtcgtggg
     1501 cgacggaacc acgccagaac aactcgaggc caacgcgcgc tcggtgctcg ggcgggcggc
     1561 gaaggtgcta aggggcgaag aaagctctct gctcgccgtg gaggaggaga tctaccgcaa
     1621 tatcgagagc tcctacgagc cccttaaccc ccaccccgac gagtccttca gggccggcgt
     1681 cgctctgtgc aaggcggccg tcacggtcgt ggtgaagaac ctcgtgccag ggacctcccc
     1741 ggagaggatc gccggggtag ccctggccgt gcaggagatc gtcatggacc gcgtctccag
     1801 gatggtgatg gcctcatacg tcgactacct gctgaccaag gtcagggaga cccaattcga
     1861 ggagcggcgg cgcttctccc gcgagctgca cgaccgcctc gcccacgaca tgacggtcgt
     1921 catccagagc ctcgagctgt accgggcgta caaggaaagc gccccggagc gcgcggagag
     1981 gcggctggtc ctcgccaggg agtcggccga cgaggccctg gacctgatgc gcgagttctc
     2041 caaggagctc aggctcttgg agacgtcgga tggcctgagg atagcgctgg agaacctgat
     2101 gaggatcagc gtaccagaag gcgtgcactc agaagtctcc ctggaaggtg aggaaaggca
     2161 tctccccgac tacgtgcgcg accagctcta catggtcttg agggaggggg tcaggaacgc
     2221 ggtcgcccac tccggctccg agcggataga ggtcgagatc gctataaccc cgaggcacgt
     2281 cacggcccgc gtccgcgatc atgggacggg actcgacccc gaggacggga ccacagaagg
     2341 cctgggcctg agctcaatgc aggagcgcgt ctcgttgctt gggggctcct tcgaactgat
     2401 ccccgaaccg ggcggcggtg cgcgggcaga cgtcagcctg cccctgatgc gggagaaggg
     2461 ccgatagacg cccgcccctc aaccggccgt cccataaggg tgctcctcgc cgatgaccat
     2521 gccctcgtgc gccagggcat ggcagagatg ctctccaccg accccggaat ggaggtggtc
     2581 gcccaggccg cgaacggaag ggaggccgtg gacctagccc gcgagcagag gccggacgtc
     2641 gtcgtactcg acgtggagat gccggtgatg agcgggcagg tggccttacg gcggctgcta
     2701 gacctccggc cgccgcccaa ggtcgtcatc gtcaccgtct tcgccgacga gtcccacgtg
     2761 cgcgagctcc tcgcgctagg ggcgggcgcc tacctctcca agaactcctc tatgagggat
     2821 ttgatctcca cggtgcgctc cgtcgcccct gggggctggg aaggccaaaa cgaggacgac
     2881 gtggtcctgt tcgtacccag gacagcgttc gaggagaacg accccgagga gagctcgctc
     2941 tccgagcggg aggcagaggt gctgatgttg gcggcgcgag ccctctccaa ccgcgagata
     3001 gccgcctcgc tctacctctc cgagacgacc gtcaagcgcc acctctccaa cgtctacggg
     3061 aagctgggcg tccgcacgag ggccgaggcg gcgaggaagg cgctctcgga aggttggatc
     3121 tccgcctggg acgtctccag ggaagtgtag ggcccgacga acgtccccgc ggggcgacgg
     3181 gcggagggtg cttgcatcat tcagatcatt tattcgctga aagccgggaa gtcggcaggc
     3241 acgcgtgtac actagctgga cacggtcgcc caagcggtgg cctgggccaa tttggtggac
     3301 ctacgtctgt gctcacttcc ggcggatgtt tctccgcagg ttaggagtac tatgcgcatc
     3361 ttgatagcca acacgccgct tatgtacagg gagactctgg ccctggcggt ccatcgtcac
     3421 aacccgggct tcgaggtgat gatggccaac cctgcctcca tggatgggga ggccaagcgc
     3481 ttcaaccctc acgtgctcat ccgggatgac gacgggatcg aggtagcctc gccggacggc
     3541 gtggtgtgct gggtcggcat catcatcgac gaccacctca aggcacgcat cagcctggac
     3601 gggaaggttt ctgagatcca cgaggtgagc ctgtacgaac tgctcgccgc cctcgacgag
     3661 gtcacggagt tcgtttccga taacagcatc cggtagcggg tgccgctacc gacagcgggt
     3721 aagtgacccc gctactctgt gtcctcggtg acgtcgctaa cggtgatcca gtcctcctgg
     3781 agagccttcc tggttgcctc tccccgggag gacaccctca tcttggcgta ggtgttcgcg
     3841 aggtggcgcc tgaccgtcgc ctcggcaatg tgaaggttct cgcttatctc gcggttggag
     3901 aggccgcggg cggccaggag caggatctcg agctccctcg ccgaaagcac gccccccgaa
     3961 cccttcctgg cctcttcgag catctccctc ggcatgccca ccaccacgtt ctcgcctttc
     4021 gggtcgaaga cggccgcgcg aaccgcgccg atcaggtgct gggtcgaggc gctcttgacg
     4081 aggtaggcgc tcgccccgag gctcatgagc tcgcgtaggt agcgggggct ctcgaacatc
     4141 gtgacgataa cgaccttcgg cgggggcaag acggcgcgca tcctcgtgag gagggtcctg
     4201 gccttctcga acggcagctg cacctgcatc aagacgacgt cgggcctggt cctctcggcc
     4261 agggcaacgg cctcttcgcc gttgggtgcg ctgccgacga cctccatgcc cccgtaggag
     4321 gcgaggatgc cggcgagccc ctcccggaac atggtgtggt cgtccgcaag gagcaccttg
     4381 atcggatcgt ccggcaagct gcgtcccttc ctcgtgtggc ccgaaccggt aatccttatt
     4441 ctagcccgcg tggacggtca ccgccaatag gaggagggct gactcacctc tcccgcgctc
     4501 gggacgcggt ctggcaagct gcacgggccg tttcgatccg agacctgaaa gaggacaacg
     4561 aatagccttc ggcgccttaa gggcgttcgc cgtttcctac accttccggt ccgtttcgtg
     4621 catacatcta tgcatttatt tttggtggcg ttctttacga gtattctcgc gcaccgtcac
     4681 ggtgtatctt gaggtgcctg tggccggcac cagaccccga cgaacggtgt cccggcaacg
     4741 cgaaagaggg gatggtcgga tgatcgacaa cgcgatcgaa ggacgcaccg aggcgagatc
     4801 gaggttcgaa tcttcctggt tgatagtgag ggacgtcatc ggagccggag gtggcctgcc
     4861 ggagatcctc acgctagaaa ccggaggcaa agccaaggtc aacggtagga tcctgcccgt
     4921 cttcagtttc gaggaagagg ctcgcctctt cctgcaccta tgcggcttgg ggggctggtg
     4981 gcacgccact aagagcggcg ccgcggagct cgcgttggtc ctctccgacg attgctcgga
     5041 cgtcgggcgc gtcgccctcg accccatccc cgagatcggc ctgtgcgggc cccacgatct
     5101 cgtcagccta ccgcgaaaag agttcgtccg gctgctcgcc gccgggagat gatgccagca
     5161 cggcgccgtc gcaagacgcg gcgcggatat caggtaagac gccgcgctgc ggcgattgga
     5221 aaccacgaaa gaaggtgcga gtgacggaca cgaaggcggg caaggaagca tcgcatcgcg
     5281 aaggtacggg cgagcaggag cagcagctag gtgtggtgtt ctgggtaacg gtcgcgatct
     5341 ccgcgttatt cgtactgtgg ggcgtgctct tcacggagaa ctttgcgagc gcgacggcga
     5401 cggttgttgg ttggatcacc gagggcctcg gttggctcta catgctcatc actacgttct
     5461 ttctcgcctt cgtcatctac ctggcctttt ccaggtacgg caagatcaag ctcggcaagc
     5521 ccgacgagga gccggagttc ggctacttcc cgtggttttg
//
LOCUS       DBNGVD010000395         5483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1916344, whole genome shotgun sequence.
ACCESSION   DBNGVD010000395 DBNGVD010000000
VERSION     DBNGVD010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1916344"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1444
                     /gene="accC"
                     /locus_tag="AC16CM_10680"
     CDS             101..1444
                     /gene="accC"
                     /locus_tag="AC16CM_10680"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3140339.1"
                     /translation="MLKKILVANRGEIALRIIRACQELGISSVAVYSEADEEALHVRH
                     ADEAVNIGPPPAGKSYLDVEAILEAARETGADAIHPGYGFLAENARFAAACRDARLVF
                     VGPASEAIEKMGNKSAARRLAEEADVPVVPGSEESASADAAVETAGEIGYPVMVKAAA
                     GGGGRGIRVAENEEELRESVRVAKREAESAFGDGSIYLEKFLAGPRHVEVQVMADNEG
                     NVIHLYERECSMQRRRQKVLEEAPSPGISDETRERICEAAVRLARESGYANAGTVEFL
                     VDGDDFYFIEMNTRIQVEHPVTEMLTGVDLVKEQILVASGEPLSLEQEDVPFVGHAIE
                     FRINAEDPDQDFMPSPGEITSLEVPGGPGVRIDSAIYQGYKIPPFYDSLVGKLIVWGL
                     DREEAIHRSGRALREYRLEGIKTTIPLHLRLLAEDAFRSGEYHTGYLEELLGEGG"
     gene            1526..2446
                     /locus_tag="AC16CM_10685"
     CDS             1526..2446
                     /locus_tag="AC16CM_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3140340.1"
                     /translation="MNRAHYATKGRAPLGEFFLLLAAFFVGTDFVSVKYALQGLPPLV
                     LVPLRYVVAGVAILVLLRMFETREGIGLTPKNLAVMAGLGFVGVTLNQVGYTVGLSLT
                     SGSHGALIFATAPIWGLILGIVLGLERGTWRGAVGLGFAVAGVALVVGDGLGSPEASV
                     GGDLLVCLSAVSWGAYTILSLPALARFDPLPVAGWTMLLGGLAAFPLALTGFPGLSEP
                     LSSVRWDAVSAGSWAAVFYSTVLASAFAIAAWQVNVSRLGANRVLVYMYLVTLFGLAS
                     SILLLGETLSVAKIAGALVILVGVYLARRA"
     gene            2570..3448
                     /locus_tag="AC16CM_10690"
     CDS             2570..3448
                     /locus_tag="AC16CM_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG/SRA domain-containing protein"
                     /protein_id="HIR3140341.1"
                     /translation="MAERLFGHVPGYPEGSLFENRAELSESRVHVPIQAGIAGSQTEG
                     AESIVLSGGYEDDADYGDVIIYTGQGGRDPRTGQQTHDQPFTRGNRALALNKLNGLPV
                     RVVRGSRHDSPDSPPAGYSYDGLYAVEDYWHEVGRSGFKIWRFHLVRIPQKMPGHKIA
                     EEPAEYSVPDRRETRVSRIIRDSAQARAVKALYKSGCQMCGTRLQCPAGPYAEAAHIR
                     PLGMPHNGPDTQSNILCLCPNHHVLFDNGAVSIAEDLTLIGMEGRLFVHGSHDIDGRH
                     LGYHRDHLLVALAGRP"
     gene            complement(3487..3906)
                     /locus_tag="AC16CM_10695"
     CDS             complement(3487..3906)
                     /locus_tag="AC16CM_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140342.1"
                     /translation="MQRTLFEKMGLKGGTRSILVNAPDAARAKIDPSTLDVAPELAGK
                     FGYIHLFARDRTELDKAFPELRAHLGPKGMLWVSWPKNKKLGTDLTLPKIIEIGYGHG
                     LVESKCLSVDTTWSALKFTHPREGKAYNNSYGRLPLP"
     gene            complement(3921..4265)
                     /locus_tag="AC16CM_10700"
     CDS             complement(3921..4265)
                     /locus_tag="AC16CM_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HIR3140343.1"
                     /translation="MAKKQKKALKSLEKDVARLRKRNDKLAEAIEKTREDQAAAYREL
                     RSLLEERLPVRSPGIGQDDSLQDGDSAPEVTPAAARRSGELGVDLSDVQGTGSRGRIL
                     IADVEAAADQDT"
     gene            complement(4295..4882)
                     /gene="leuD"
                     /locus_tag="AC16CM_10705"
     CDS             complement(4295..4882)
                     /gene="leuD"
                     /locus_tag="AC16CM_10705"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3140344.1"
                     /translation="MDPVERVSGTALPLGYSDVDTDQIVPSDALKRIERTGFGQFLFS
                     SWRDDEDFIMHKEEHKDAVILIAGENFGSGSSREHAVWAIKDYGFDAVIAPSFADIFR
                     NNSTKNGLLAIDLPEDTVKKLLQAVRDDPETLITIEMAELTVTAPNVHETFEMDDFTR
                     QRIMEGLDDISLTLTHEQDLDAFERSRPAYLPSVK"
     gene            complement(4883..>5483)
                     /locus_tag="AC16CM_10710"
     CDS             complement(4883..>5483)
                     /locus_tag="AC16CM_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3140345.1"
                     /translation="TDEGAEFDKEVVIQAEDLVPYVSWGTTPAQTVSLDGEVPEPENE
                     GHERALRYMALEPGTAIRDIEVDTVFLGSCTNSRIEDLRAAARVLEGHKVKEGIRAMV
                     VPGSMRVKKQAEEEGLAEIFTDAGFDGRNAGCSMCLGMNPDILQPGERCASTSNRNFE
                     GRQGKGGRTHLVSPAVAAATAVMGRFASPSELGVPLEVV"
BASE COUNT          917 a         1704 c         1761 g         1101 t
ORIGIN      
        1 cgaaaatccc ggtcaatgta tctttagtcg cggacgtgcg ataacttcgg ggctggacca
       61 cgtcctgatg ctatgggatc cagagggagg acgatacacc atgttgaaga agatattggt
      121 cgccaaccgc ggtgagatcg cgctgcgcat catccgggcg tgccaggagt tgggcatttc
      181 ctctgtagcc gtctactccg aggctgacga ggaggccctc cacgtgcgtc acgctgacga
      241 ggccgtcaac atcggtccgc ccccggcggg caagagctac ctggatgtgg aggccatcct
      301 cgaggccgcc agagagacgg gcgccgatgc gatccatccc ggctacggtt tcctggccga
      361 gaatgcccgc ttcgcggcgg cctgccggga cgcgaggctg gtcttcgtcg ggccggcgtc
      421 cgaggcgatc gagaagatgg gcaacaaatc ggcggcgcgg cggctggcag aagaagcaga
      481 cgtgccggtt gtgcctggct cggaggagtc agcttcggcc gacgcggcgg tcgagacggc
      541 gggggagatc ggctacccgg tcatggtgaa ggcggcggcc gggggaggcg gtcgcggcat
      601 cagggtggcc gagaacgagg aggagcttcg cgagtccgtc cgggtcgcca aacgggaggc
      661 cgagtcggcg ttcggggacg gctcaatcta cctcgagaag ttcctggcgg ggccccgcca
      721 cgtcgaggtg caggtcatgg cagacaacga gggcaacgtg atacacctct acgagcggga
      781 gtgctccatg cagcgccgca ggcagaaggt gctggaagag gcaccatctc ctggcatcag
      841 cgacgagacg cgcgagagga tatgcgaagc agccgtcagg ctggcgcggg agtcggggta
      901 cgccaacgcc gggacggtcg agtttctggt cgacggggac gacttctact ttatcgagat
      961 gaacaccagg atacaggtcg agcaccccgt tacggagatg cttacgggcg tcgacctggt
     1021 gaaggagcag atcctggtcg cctccggcga gcctcttagc ctcgagcaag aggatgtgcc
     1081 tttcgtgggg cacgcgatag agttccgaat caacgccgag gacccggatc aggacttcat
     1141 gccatcaccc ggcgagataa catccctgga ggtgccgggc ggtcccggcg tgcggataga
     1201 ctcggcgatc taccaggggt acaagatccc gcccttctac gactcgctgg ttggcaagct
     1261 gatcgtctgg ggcctggaca gggaggaggc gatccaccgc tcgggccgcg cgcttcgcga
     1321 ataccgcctg gaaggcatca agaccacgat cccactccac ctgcggctcc tcgcagagga
     1381 tgccttccgc tccggcgagt accatacggg ctacctggag gagctgctgg gggagggagg
     1441 ctagggatct tgccgggagt tccgggggga gaaaaggcaa caggtttcag gcttcagtag
     1501 aagtacctga tacctgttgc ctcccatgaa tcgagcgcac tacgccacga aagggagagc
     1561 accgctcggt gagttcttcc tgcttctggc cgccttcttc gtcggcacgg acttcgtctc
     1621 ggtcaagtac gctctgcaag ggctgccgcc gctcgtcctc gtgccgctca ggtacgtggt
     1681 cgccggggtc gcgatcctgg tcctgctccg catgttcgag accagggaag gcataggcct
     1741 caccccgaag aaccttgcgg tcatggcggg cctcgggttc gtgggcgtta ccctaaacca
     1801 ggtcggttac accgtcggcc tgagcctcac cagcggttcc cacggcgccc tcatcttcgc
     1861 caccgccccg atatggggcc tgatcctcgg catcgtgctc ggtctcgagc gcggcacctg
     1921 gcggggcgcc gttgggctcg ggttcgccgt ggcaggcgta gcgctggtgg tgggtgacgg
     1981 tctgggatcg ccggaggcgt ccgtgggagg agacctgctc gtttgcctga gtgccgtgtc
     2041 ctggggcgcg tacacgatcc tctccctccc cgccctcgcc cgcttcgacc cgctacctgt
     2101 ggccggatgg accatgctgc tcggcggcct cgcagcgttc ccgctcgccc tcaccggctt
     2161 tcccgggctc tccgagcccc tctcttccgt gcggtgggac gccgttagcg cgggatcctg
     2221 ggccgcagtc ttctattcga cggtgctggc aagcgccttc gccatcgccg cctggcaggt
     2281 caacgtctcc cgactggggg ccaacagggt gctggtgtac atgtaccttg tgaccctgtt
     2341 cgggctcgcc tctagcatcc tgctgctcgg cgagacgctc agcgtcgcaa agatagcggg
     2401 agccctggtg atcctggtag gcgtctacct ggcccgccgc gcctgaccct tgctcgccct
     2461 aattggtcgg tccgggccga tctggcttgt agccgatcgg tcccatacaa tgttgatcga
     2521 tgctcgaaac tcgatgctcc cgcgaacgag ggatctggta ggccccggca tggcggaacg
     2581 cctctttgga cacgtacctg gttatcccga aggaagcctc ttcgaaaacc gcgcggaact
     2641 cagcgagagc agagtccacg tgccgatcca ggcgggcatc gccggaagcc agactgaggg
     2701 ggcggagtct attgttctct ccggtggcta cgaagacgat gcagactacg gggacgtgat
     2761 catatacacc gggcagggtg gtagagaccc ccgtaccggg caacagaccc acgaccagcc
     2821 cttcacccgt ggtaaccggg cgctcgcgct caacaagttg aacggcctac cagttcgcgt
     2881 tgttcgaggt tcaaggcacg actccccgga ctcaccgcca gctggttaca gctacgatgg
     2941 actgtacgcg gtcgaagatt actggcacga ggttggtaga tcgggattca agatctggcg
     3001 tttccacctg gtaaggattc cccagaagat gccgggtcat aagatcgccg aggaaccggc
     3061 tgagtattct gttccggacc gtcgagaaac gagagtatcg cgaattatta gggattcggc
     3121 acaggcgaga gctgtgaaag cactctacaa gagcggatgc cagatgtgcg gaacccgttt
     3181 gcagtgtccc gccggtccat atgcagaagc tgctcatata cgcccgctcg gtatgcccca
     3241 caacggtccg gacacccaga gcaatatcct ctgcttatgt ccgaaccacc atgtgttgtt
     3301 cgacaacggt gccgtctcga tagcagagga tctgaccttg atcggcatgg agggccgcct
     3361 tttcgttcat ggaagccacg acatcgacgg gcgtcatctc ggctaccatc gtgaccatct
     3421 gctcgtggcc cttgcaggtc gaccctgaga tatccctgcc cctatggcta gccgtggatt
     3481 cccggtttac ggtagcggga gccttccgta gctgttgttg tacgctttgc cttccctggg
     3541 gtgggtaaac ttcagggcgg accacgtggt gtccacgctc aggcatttgc tctccactag
     3601 cccatggccg tagccgatct ctatgatctt cggcagcgtc aggtcggtcc cgagcttttt
     3661 gttcttcggc cacgagaccc agagcatgcc cttcggcccg aggtgggccc tgagctctgg
     3721 aaacgcttta tctagctccg tcctgtctct ggcgaagagg tggatgtagc cgaactttcc
     3781 ggccaattcc ggggcgacat cgagggtgga cgggtcgatc tttgcccgcg cggcatcggg
     3841 tgcgttcacg aggatcgagc gggttcctcc cttcaggccc atcttctcga acaatgttct
     3901 ctgcaaggta gcccggactt ctaggtatcc tgatccgctg ccgcctccac atccgcgatg
     3961 aggatgcgcc ctcttgatcc cgtaccctgc acgtcagaga gatccacgcc aagctccccg
     4021 gatcgccggg cggcagccgg cgtgacctct ggcgcgctgt cgccgtcttg caagctgtcg
     4081 tcctgcccga tgccagggct ccgcacaggc agccgctcct cgaggaggct gcggagctcc
     4141 cggtatgccg cggcctggtc ctcccgcgtc ttctcaatgg cttctgctag cttgtcgttc
     4201 cgtttgcgta gcctggccac gtctttctcg aggctcttga gggccttttt ctgtttcttt
     4261 gccatcttcg ttaccgcctg ttcctcgaaa ctggttactt cacgctaggt aggtaggctg
     4321 gccgcgagcg ctcgaacgcg tcgaggtcct gctcgtgggt gagggtaagg ctaatgtcgt
     4381 cgaggccctc catgatgcgc tggcgggtga agtcgtccat ctcgaaggtc tcgtggacgt
     4441 ttggggcggt gaccgttagc tcggccatct cgatggtgat cagggtctcg gggtcgtccc
     4501 tgacggcctg gagtagcttc ttgaccgtgt cctcgggcag gtcgatggcg aggagcccgt
     4561 tcttggtcga gttgttcctg aagatatcgg cgaaggaggg tgctatcacc gcgtcgaagc
     4621 cgtagtcttt tatcgcccag acggcgtgtt cgcggctgga ccccgagccg aagttctcgc
     4681 cggcgataag gatgacggcg tctttatgct cttctttgtg cattatgaag tcttcgtcat
     4741 cacgccagct ggagaacagg aactgcccga agccggttcg ctcgatgcgc ttgagggcgt
     4801 cgctgggtac gatctggtcg gtgtcaacat ccgagtaccc gagcggaagc gccgtccctg
     4861 atacgcgctc gacgggatcc atctacacca cctccaacgg tacgccgagc tcgctcggcg
     4921 aggcgaaccg gcccatgacg gcggtcgccg cggctactgc ggggctgacg aggtgcgttc
     4981 tgccgccctt gccctggcgt ccctcgaagt tgcggttgct ggtcgaggcg caccgctcgc
     5041 cgggctgcaa aatgtcgggg ttcatgccga ggcacatgga acagcccgcg ttgcgcccgt
     5101 cgaatccggc atccgtgaag atctcggcca gcccctcctc ctcggcctgc ttcttgacgc
     5161 gcatggaacc gggcacgacc atcgcccgaa tgccttcctt gaccttgtgg ccttcgagca
     5221 cgcgggccgc ggcgcgcagg tcctcgatgc gtgagttggt gcaggacccc aggaaaactg
     5281 tatccacttc tatgtccctt atcgccgtcc ctggctccag cgccatgtac ctgagcgccc
     5341 tctcgtggcc ttcgttctcg ggttctggca cctcaccatc gagcgacacg gtctgggccg
     5401 gcgtcgtgcc ccacgagacg taaggaacga ggtcctccgc ctgtatgact acttccttgt
     5461 cgaactcggc gccctcgtcc gta
//
LOCUS       DBNGVD010000396         4507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3555483, whole genome shotgun sequence.
ACCESSION   DBNGVD010000396 DBNGVD010000000
VERSION     DBNGVD010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3555483"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /locus_tag="AC16CM_10715"
     CDS             <1..2034
                     /locus_tag="AC16CM_10715"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3140346.1"
                     /translation="SAASRPGDAWVGDVKYHLGIRGFQLGEEEADEEVFIHLAPNPSH
                     LEHVNPVVEGMARASQEERTEAGPPRQDLEASLPIILHGDAAFPGEGVSAETLNLSRL
                     PGYMTGGTIHIITNNQLGFTTERRDARSTTYASDLAKGFEIPVVHVNADDPEACMAVT
                     RIAHAYREKFNKDFMIDLVGYRRFGHNEGDEPAYTQPLMYEIIRSHPTVRELWAEELE
                     RRGVIEEGEAQEMLDKVMDEMAEIQNKPSDELEDEDFAADELYTPLVDIPETAVPAEE
                     LSELNVALLETPEGFTMNSKLGRLFQKNRGDLDQIDWAHAEALAFASLLEDGIPVRLT
                     GQDTERGTFSQRHAVLYDAETDERYVPLQNLPQADASFSIYNSPLSEIAVLGFEYGYS
                     VNSGNALVLWEAQYGDFANVGQPMIDQFIVSGQAKWGQTSGLVLLLPHGYEGQGPEHS
                     SARLERYLQLAGHENVRIANLTTAAQYFHLLRAQAFLEDNKRPLIIMAPKSLLRHPMA
                     ASTLEDLAEGSFRPVLDDEEAQKRADSVERLILCSGKIHTELAGSEYREEAVDTAIVR
                     IELLYPFPEDQIKGVIEGYPNLREILWVQEEPQNMGAWTFVEPRLHALTGGELPLHYI
                     GKQPRPSPAQGSAKFHRQEHAGIVRDAFETVERNLDEESAVKETQVRASAPSE"
     gene            2052..3341
                     /gene="odhB"
                     /locus_tag="AC16CM_10720"
     CDS             2052..3341
                     /gene="odhB"
                     /locus_tag="AC16CM_10720"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3140347.1"
                     /translation="MAAEIHVPELGESVADATVGRWLKQEGDAVTSGEALVELETDKI
                     NFEVEAERDGVLESISRGEGDTVTVGEVIGTIGEGDGAAAAPADGQEEQPQAEVEQPA
                     AEEADETSNGHREVEEVAGRASPSVRKLAQEYEVDLSEVSGSGSGGRITRDDVERLIR
                     AQSNTAASERTAAPEEPAGDGRAETQPAPAASQNGRADQEERVRVSRRRQTIAQRLVE
                     SQQATAMLTTFHEADMSAVMELRRRRKEAFQERTGARLGFMSFFTKAAIGALKAFPKV
                     NAELQDTELVLKKYYDVGVAVSTDEGLVVPVLRDADHKNFAEIEQGIADLATKARDGK
                     LTLDDLRGGTFTITNGGIFGSLVSTPILTLPQVAILGMHKIQERPVVVDGEIVVRPMM
                     YLALSYDHRVIDGAEAVSFLVRIKDLIEDPESLLLEG"
     gene            3441..4184
                     /locus_tag="AC16CM_10725"
     CDS             3441..4184
                     /locus_tag="AC16CM_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3140348.1"
                     /translation="MGDVGKAMGKATLQTLVSEARRLRAQENWGTEAIIANTNILDLD
                     PTNVAALNRRARCYWEQTNLIAAEKDYRRALGLRPGNKHIEEALQEIWEKAREEREHE
                     EQVDKVRAISNFAQAYAIGRDYKNKPPAKRQIAIEAFRQAFRLNNDKTDVLIELAAVH
                     RSLRQRDEAERIYAWILRRERNSAAKVGLAAIYKDKKRLRDGLGLCDEVLADEPHNPY
                     ALRCRASILSELDRGAEAAETFGRSFGQR"
     gene            complement(4175..>4507)
                     /locus_tag="AC16CM_10730"
     CDS             complement(4175..>4507)
                     /locus_tag="AC16CM_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3140349.1"
                     /translation="VRERLYGSDEGPHVPHLFEIEVLGALRRHALISDLSAKRGQELL
                     ADLTSMRLNRYPHTALLSRMWELKENVTIQDAAYIALAETLDAPLITMDGRLARAPGI
                     RAVVEVYR"
BASE COUNT          940 a         1435 c         1451 g          681 t
ORIGIN      
        1 tccgcggcga gccgccccgg cgacgcctgg gtcggcgacg tgaagtacca cctcggcatc
       61 aggggcttcc agctggggga ggaggaggcc gacgaggagg tctttatcca cctggcccca
      121 aacccaagcc acctcgagca cgtgaacccc gtggtcgagg gaatggcccg cgcctcccag
      181 gaggagcgga ccgaggcggg accgccgcgc caggacctcg aggcctcgtt gcccataatc
      241 ttgcacgggg acgcggcgtt ccccggggag ggcgtctcgg ccgagacgct caacctgagc
      301 cgcctgccag gctacatgac cggcggcacc atacacatca tcactaacaa ccagctcggc
      361 ttcactaccg agagacgtga cgcccgctcc accacctacg ccagcgacct ggccaagggg
      421 ttcgagatcc ccgtcgtcca cgtcaacgcc gacgacccgg aggcgtgcat ggccgtcacc
      481 aggatagccc acgcctaccg cgagaagttc aacaaagact tcatgatcga cctcgtcggc
      541 taccgccgct tcgggcacaa cgagggtgac gagcctgcct acacgcagcc cctgatgtac
      601 gagatcatcc gcagccaccc cacggttcgt gagctgtggg ccgaggagct ggagcgccgc
      661 ggcgttatag aagagggcga agcccaggag atgctcgaca aggtcatgga cgagatggcc
      721 gagatccaga ataaaccatc ggacgagctt gaagatgagg acttcgccgc tgacgagctg
      781 tacacgccgc tggtggacat cccggagaca gccgtcccgg cggaagagct ctccgaactg
      841 aacgtcgccc tgctggagac ccctgaaggc ttcacgatga actccaaact cggtcggctc
      901 ttccagaaga accgtggaga cctcgaccaa atagattggg cgcacgcgga ggcgttggcc
      961 ttcgcctcgc tgctcgagga cggcatcccc gtgcggctca ccgggcagga cacggagcgc
     1021 ggaaccttct cacagcgcca cgccgtgctc tacgacgccg agaccgacga gcgctatgtc
     1081 ccgcttcaga acctgccgca ggccgatgct tcgttctcca tctacaacag cccgctctcg
     1141 gagatagcgg tgctcggctt cgagtacggc tactccgtga actcggggaa cgcgctcgtg
     1201 ctctgggaag cccagtacgg ggacttcgcc aacgtggggc agcccatgat cgaccagttc
     1261 atcgtgagcg gccaggcgaa gtggggccag acctcgggcc tcgtcctgct cctgccgcac
     1321 ggctacgagg gtcaggggcc agagcactcc agcgcccgcc tggagcgcta tctccagctc
     1381 gccggacacg agaacgtgcg catcgccaac ctcaccacgg cggcccagta cttccatctt
     1441 ctgcgcgccc aggccttcct cgaggacaac aagcggcccc tgatcatcat ggcgcccaag
     1501 agcctgctca ggcaccccat ggccgcctcc acgctggagg acctcgccga gggctccttc
     1561 cggcccgttc tggacgacga ggaagcccag aagcgggctg actcggtcga gcgcctcatc
     1621 ctgtgctcgg gcaagataca taccgagctc gccgggagcg agtaccgtga ggaggctgtg
     1681 gacacggcca tcgtcaggat agagctcctc tacccattcc cggaggacca gatcaagggc
     1741 gtgatcgagg gctacccgaa cctgagggag atcctctggg tgcaggagga accgcagaac
     1801 atgggcgcct ggacgttcgt cgagccgcgc ctgcacgcgc tgacgggcgg cgaattgccc
     1861 ctccactaca tcggcaagca accgcgcccg agccccgcgc agggctccgc caagttccac
     1921 aggcaggagc acgccggtat cgtgagggac gctttcgaga ccgtcgaacg caacctagac
     1981 gaagagagtg ccgtgaaaga gacgcaagta cgcgcctcgg ccccgagcga gtaagagagg
     2041 aagagtgagt ggtggctgct gagatacacg ttcccgagct tggggagtct gtcgccgacg
     2101 cgaccgtcgg acgttggctc aagcaagagg gcgatgccgt aacctccggc gaagcgctgg
     2161 tcgagctaga gaccgacaag atcaacttcg aggtcgaggc cgaaagggac ggcgtgctcg
     2221 agtccatctc caggggcgag ggcgacaccg tgaccgtcgg cgaggtcata ggcactatcg
     2281 gcgagggaga cggagccgcg gcggcccccg cggatgggca ggaagagcag ccccaggccg
     2341 aagtggagca gcccgcagca gaagaggcgg acgagacttc caacggtcac agggaggtcg
     2401 aggaggtcgc gggccgggca tcgccctcgg tcaggaagct ggcccaggag tacgaggtgg
     2461 acctgtccga ggtctccggt tcggggtcgg gcgggcgcat cacgcgcgac gacgtggaac
     2521 gcctcatcag ggcccagtcg aacaccgcgg cctccgagag gaccgcagcg ccggaggagc
     2581 ctgcgggcga cggcagggcc gaaacgcagc ccgctccggc cgcctcccag aacggaaggg
     2641 ccgaccagga ggagagggtg cgggtctccc gccggcgcca gaccatcgcc caacggctcg
     2701 tggagtcgca gcaggcgacg gccatgctca ccaccttcca cgaggcggac atgagcgcgg
     2761 tcatggagct caggaggcgc cgcaaggagg ctttccagga gcggaccggc gcccgactcg
     2821 gcttcatgag cttcttcacc aaggcggcca tcggcgccct caaggccttc cccaaggtga
     2881 acgccgagct gcaggacacc gagctcgtgc tcaagaagta ctacgacgtg ggcgtggccg
     2941 tgagcacgga cgaggggctc gtcgtccccg tccttcgcga cgccgaccac aagaacttcg
     3001 ccgagatcga gcagggcata gccgacctcg ccaccaaagc ccgcgacggc aagctgaccc
     3061 tcgacgacct gcgcggcggc actttcacca tcaccaacgg cggcatcttc ggctcgctgg
     3121 tctccacgcc catactcacc ctcccgcagg tggccatcct ggggatgcac aagatccagg
     3181 agcgccccgt ggtcgtggac ggcgagatag tcgtgcgccc catgatgtac ctggccctct
     3241 cctacgacca ccgcgtaata gacggcgccg aagccgtaag cttcctggtc aggatcaaag
     3301 acctcatcga agaccccgag tccctgctgc tcgaagggta gatgccggta gccgaatccg
     3361 aggtgttcga ggccccttag ggggcctcct tcgtcgttgc gtcgaacccc aggacaaata
     3421 taatctcgaa gtccgcgccg gtgggtgacg tggggaaggc aatgggtaaa gctactttac
     3481 agactcttgt gtcggaggcc cgaagactgc gcgcgcaaga aaactggggc accgaagcca
     3541 taatcgcgaa tacgaacata cttgatctag atcccaccaa cgtggctgcg ctgaaccggc
     3601 gtgcgaggtg ctattgggag cagacaaacc tgattgctgc cgagaaagat taccggcgtg
     3661 cgcttggact tcgtcctgga aacaagcata tagaggaggc gctccaggag atttgggaga
     3721 aagcgcgcga agagcgtgaa catgaagaac aggtcgacaa ggtgcgggcc attagcaact
     3781 ttgcgcaagc ctatgcgatc ggtcgagact acaagaacaa acctcccgcc aagcgtcaga
     3841 tagccataga ggcgttcaga caagccttcc gactgaacaa cgacaagacg gacgtactga
     3901 tcgagcttgc tgccgtccat cgcagtctac ggcagaggga cgaggcggag agaatctatg
     3961 cctggattct tcgtcgtgag cgaaactccg cggcaaaggt tggccttgcg gccatctaca
     4021 aagacaaaaa gcgcctcaga gacgggcttg gactctgtga tgaggtgctt gcggatgagc
     4081 cacacaaccc gtacgctctg cggtgccgcg cgagcatact ctcggaactg gatcggggag
     4141 cagaggctgc ggagactttt ggcagatctt tcggtcagcg gtagacctcg accaccgcgc
     4201 gtattccggg ggctcgtgcc agacgcccat ccatcgtgat cagcggtgca tcaagggtct
     4261 cggcgagggc gatgtaggcg gcatcctgga tggtgacgtt ttctttcaac tcccacatgc
     4321 gagaaagcag cgccgtgtgc gggtatcggt ttagcctcat gctcgtaagg tctgcaagga
     4381 gctcctgacc tcgcttggcg gaaaggtcgc ttatgagtgc gtggcgacgc agagcgccca
     4441 atacctcgat ctcgaacaga tggggtacgt gtggtccttc atccgagcca tacagccgtt
     4501 ctcggac
//
LOCUS       DBNGVD010000397         6924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1188083, whole genome shotgun sequence.
ACCESSION   DBNGVD010000397 DBNGVD010000000
VERSION     DBNGVD010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1188083"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC16CM_10735"
     CDS             <1..515
                     /locus_tag="AC16CM_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140350.1"
                     /translation="MGTLSVRYTDRLVMRFGARKLVLFGLVLFMAGLALFTRAPIDGG
                     YVVDILPVMVLLGVGAGLCFPALMTLAMSGATPDDAGLASGLINTTAQVGGALGLAVL
                     ATLSASRSVELAGAGESRVAALLGGYHLAFGIGAALVLVAITVAATILQPAPRSEPGT
                     EADPYECEAA"
     gene            551..1096
                     /locus_tag="AC16CM_10740"
     CDS             551..1096
                     /locus_tag="AC16CM_10740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RBBP9/YdeN family alpha/beta hydrolase"
                     /protein_id="HIR3140351.1"
                     /translation="MATRQVLFVHGAGGEAFGEDEALVRSLREALGPDFAVEYPEMPD
                     GDAPEFGKWGARISRELSAADGEVILVGHSLGASTLLKYLSEEQIERPIAGLFLIATP
                     FWGTKDWEAEYALREDFPAALPEDIPIFLYHSRDDEWVPFAHLALYAERLPQATVREF
                     EGRGHQFGDDLSEVAEDIAKL"
     gene            1112..1912
                     /locus_tag="AC16CM_10745"
     CDS             1112..1912
                     /locus_tag="AC16CM_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018831112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140352.1"
                     /translation="MNTVTSDDGTSIAFERFGAGPPVILVGGASTDRAMTRPLATELA
                     GDFTVINYDRRGRGDSGDTQPYAVEREVEDIGALIAEAGEPASLYGHSSGAALALHAA
                     ALGLPVAGLAMYEPPFSPDREEERRTSRQYGEELRAILSEGRRGDAAALFMAMVGMPE
                     EMVEGSRKAPWWPRMEAVAPTLLYDSEVMGDVSREGTIPTELLGAVGIPTLVLCGGAS
                     PGWMIDVGKQVAEGVPDGRAEVLEGQDHNVSPEVLAPVLAGFFATVGG"
     gene            1915..2691
                     /locus_tag="AC16CM_10750"
     CDS             1915..2691
                     /locus_tag="AC16CM_10750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140353.1"
                     /translation="MLLEGKNAVVYGGGGSVGGAAARAFAREGADVFLAGRTREPLDK
                     VASDIEEMGARVEVAELDALDEQAVRKHLDDLVDGAGSVDVSFNAISLRDVQLIPLAE
                     MSQDDFMRPIVTGMITHFVTAKAVARHMAAQGSGVILTFTASAVRAYAPGALVGGFGI
                     ACNAIEALTKQLAVELGPQGVRAVCLRSEGIPESWEGISTEDWSGSTDEIEASIRERS
                     LLRRVTTLADVGNAAAFLASDRAGATTGTVFNLTSGTVVD"
     gene            2706..3125
                     /locus_tag="AC16CM_10755"
     CDS             2706..3125
                     /locus_tag="AC16CM_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3140354.1"
                     /translation="MSSTSGQTRAAGGGRALTIALWVLQIILALQFVMAGLAKLFGDQ
                     AMVEMFATIGIGQWFRYVVGTLEVVGAVGVLVPRVSGLAALGLVCLMAGAVLTNLFIL
                     GASPLLPIGLLVIGALVAWGRWPQTRSLVAGSRRRVA"
     gene            3164..4276
                     /locus_tag="AC16CM_10760"
     CDS             3164..4276
                     /locus_tag="AC16CM_10760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3140355.1"
                     /translation="MIENLQQQVQGAIEELVESGTERGLQVAVYREGEQVVDAVAGLA
                     DPETGRPVSSDTPFYAYSTGKGATSAVAHVLVERGLFGYDTPVSELWPEFGTHGKEGA
                     TVRHVLTHTVGVPGIPADTMPEDLCDWDGMCAAIADSEPWWEPGTKTAYHAYPFGYIL
                     GEIARRATGKPISRVLKEEVAGPLGVADEIYFGMPESELYRLAPLEDVEGSAEFLASM
                     PDDSPFFRLGPRTVAPTAEFGNRKDILMADIPAGGKMSAGAIARTYAAMIGEVDGVRL
                     VSPGRLREISAPAFGGTDEIMGNPVTWALGYSIGRLGSDAQQSPTVFGMGGVGGSFAY
                     ADTATGVAFALTKNRLTPDFSAAQRISDMVTKAVGS"
     gene            4305..4721
                     /locus_tag="AC16CM_10765"
     CDS             4305..4721
                     /locus_tag="AC16CM_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140356.1"
                     /translation="MGLGRFKVVAGLAVSDMDRAVEFYEEKLGLTSWTGQENNRAYEC
                     GEGTVLHVYLSPDHAGGTTATLASWYVREIESLVDVLTSRGVVFERYDRPPILTDDRG
                     IATFEGGAKVAYFKDPDGNTLSLAQVRDRSPTDEDR"
     gene            4718..5278
                     /locus_tag="AC16CM_10770"
     CDS             4718..5278
                     /locus_tag="AC16CM_10770"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140357.1"
                     /translation="MRKLTAFQLMSLDGVVESPNEWAFSYSDEEMERENAAGMAASDA
                     LLLGRATYEEFASFWPYQPGGTQMVDYINGVRKHVVSTTLEEPLGWNNSTLIGGDVAE
                     EINALKEQPGENITITGSITLVRWLIRTGLLDELRLMVYPVVLGGGRRLFEDGGEPEV
                     LELASSKTYATGVVSLAYRPSGGVSS"
     gene            5296..5910
                     /locus_tag="AC16CM_10775"
     CDS             5296..5910
                     /locus_tag="AC16CM_10775"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140358.1"
                     /translation="MVVCNAVSLDGFYEGPNKNVMDLFEYRRSYPTDESFDDYNAERL
                     RAADTLLLGRTSYEGFKGFWPPVANDPDARPLQREISRLNNAIDKVVVSDGLTAGETA
                     PWGNTRIVGRADAHEQIAELKRQTGGEILVFGSRTLWNDLLEDGLVDELHLMIGPVLV
                     GAGTPLFGGRPTVTLRLLGTRTWEGSGNVLARYEVRPNDSRGEG"
     gene            5913..6323
                     /locus_tag="AC16CM_10780"
     CDS             5913..6323
                     /locus_tag="AC16CM_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140359.1"
                     /translation="MDFRIEVVTVPVTDVERSLRFYAEKMGFAVDIDHKVSDEVRLVQ
                     LTPPGSFCSIHLGRGTIATRPGDLDGVFLVVTDVRAAHADLVKKDVDVGEIQIFDDGA
                     YRPAREDENLDMVGCVFFRDPDGNRWCIQQIPAR"
     gene            6407..>6924
                     /locus_tag="AC16CM_10785"
     CDS             6407..>6924
                     /locus_tag="AC16CM_10785"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140360.1"
                     /translation="MGKVTSGLTMSLDGFIAGPNDGPEHPLGEDGMRLFDWYGGGDTE
                     YVVPSGGMTFQVSSQSAEMLRGVFSSIGAIVTGRRTFDITNGWGGRHPLDVPVVVLTH
                     NVPDGWEYEGSPFTFVTDGVESAVEKARKLAGEKHVAVGAASLVQQCLRAGLLDEVHV
                     DLVPVLLGGGVRL"
BASE COUNT         1194 a         2136 c         2426 g         1168 t
ORIGIN      
        1 tgatgggaac gctctccgtc cgctacacag accgcctggt catgcgcttc ggcgccagaa
       61 agctggtgct gttcgggctc gttctcttta tggccggtct cgcgctgttc acgcgggcgc
      121 ccatagacgg cggctacgtg gtcgacatcc tgcccgtcat ggtcctgctc ggcgtcgggg
      181 ccgggctctg tttcccggcg ctcatgacgc tcgccatgtc tggcgccacg ccggacgacg
      241 ccgggcttgc ctccggcctc atcaacacga ccgcgcaggt cggcggcgcc ctgggattgg
      301 ccgtgctggc gaccctgtcg gcttcgcgga gcgtggagct ggccggggcc ggcgagtcca
      361 gggtggcggc gctcctgggt ggttaccatc tcgcgttcgg tatcggggcc gcgctcgtcc
      421 tcgtggcaat cacggtcgcc gccacgatcc tgcaacccgc gccccgttcc gagccaggga
      481 ccgaagcgga cccttatgag tgcgaggcgg catgaacgcc caggtgcaac ccgaaggagg
      541 taggtcgggc atggctacca ggcaggtgct cttcgttcac ggtgccggcg gcgaggcttt
      601 cggggaagat gaggccctgg tcaggagcct gcgggaggcg ttgggtcccg acttcgccgt
      661 tgagtacccg gagatgcccg atggtgacgc acccgagttc ggaaagtggg gtgcccgtat
      721 ctcccgcgag ctctccgcgg cggacggaga ggtgatcctc gtcggccact cgctcggcgc
      781 gtcgaccctc ttgaagtacc tctccgagga gcagatagag agacccatcg ccggtttgtt
      841 cctcatcgcg acccccttct ggggaacgaa agattgggag gccgagtacg cgttgcgtga
      901 agactttccg gcggccctgc ctgaggacat acccatcttt ctctaccaca gccgcgacga
      961 cgagtgggtc ccgttcgcgc acctcgcgct ctacgccgaa cggcttcccc aggcgaccgt
     1021 acgcgagttc gaaggccgcg gacaccagtt cggcgacgac ctgtcagaag tggcggaaga
     1081 catcgcgaag ctctaggaag caggagggcc catgaacacc gtgacttctg acgacggcac
     1141 cagcatcgcc ttcgagcggt tcggcgcggg tccccctgtc atcctggtcg gcggggcgtc
     1201 taccgacagg gcgatgaccc gtccactggc cacagagctg gcgggggact ttaccgtgat
     1261 caactacgac cgcaggggcc gtggcgacag cggcgacacc cagccctacg ccgtggaacg
     1321 tgaggtcgaa gatataggcg cgctcatcgc cgaggcgggg gaaccagcct cgctgtacgg
     1381 ccactcctct ggcgcggccc tcgcgctcca cgcggcggcc ctgggtctac ccgtcgccgg
     1441 gctcgccatg tacgagccac ccttctcgcc cgaccgcgag gaggagcggc gtacctcgcg
     1501 ccagtacggc gaagagctca gggccatcct gtcggaggga cgccgcggtg acgctgccgc
     1561 cctgttcatg gcgatggtcg gcatgcccga ggagatggtc gagggctcgc gcaaagcgcc
     1621 gtggtggccg cggatggagg cggtggcgcc cacgctcctc tacgactccg aggtcatggg
     1681 cgacgttagc cgggaaggca ccatcccaac cgaattgctc ggtgcggtcg gcatccccac
     1741 gctcgtgttg tgcggcgggg cgagccccgg gtggatgatc gacgtcggaa agcaggtggc
     1801 agaaggcgtg ccagacggac gggcagaggt tctcgaaggc caggaccaca acgtgtcccc
     1861 cgaggtactc gcgccggtgc tggcggggtt cttcgccacc gtaggaggtt gaacatgttg
     1921 ctcgaaggca agaacgctgt ggtgtacggg ggtgggggat cggtcggcgg ggctgccgcc
     1981 cgagcttttg cccgcgaggg ggcagacgtc ttcctcgccg ggcgcaccag ggagccgctt
     2041 gacaaagttg cctccgacat cgaagagatg ggagcgcggg tcgaggttgc cgagctcgac
     2101 gccctcgacg agcaggccgt caggaagcat ctggacgacc tggtggacgg cgccggaagc
     2161 gtggacgtct ccttcaacgc catctctctc agggacgtgc agttgatccc gcttgcggag
     2221 atgtcgcaag atgatttcat gagacccatc gtgaccggta tgatcaccca cttcgtcaca
     2281 gcgaaggccg tggcccggca catggccgcg caggggtcgg gggtgatcct gacgttcacc
     2341 gcctcggcgg tccgcgcgta cgcccccggc gccctcgtgg gagggttcgg gatcgcctgc
     2401 aacgccatcg aggccttgac gaaacagctc gcggtggagc tgggtccgca gggagtccgt
     2461 gccgtgtgcc tgcggtccga aggcataccg gagagctggg agggtatctc gaccgaggac
     2521 tggtcgggct cgaccgatga gatcgaggct tccatcaggg agaggtctct gctgagacgg
     2581 gtaacgacgc tcgccgacgt gggaaacgcg gcggctttcc tggcctccga ccgtgccggc
     2641 gcgacgacgg gcaccgtctt caacctgacc agcggcacgg tcgtggattg agggaggaga
     2701 tttcgatgag cagtacttca ggccagacgc gcgcggcagg cgggggacgc gccctgacca
     2761 tagcgttgtg ggttcttcag atcatcctgg cgcttcagtt cgtgatggcc gggttggcca
     2821 agctgttcgg cgaccaggcg atggtcgaga tgttcgccac catcggcatc ggccagtggt
     2881 tccgctacgt ggtcgggacg ctcgaggttg tcggcgcggt aggggtcctg gttccgcgcg
     2941 tctcggggct ggccgcgctc gggctcgttt gcctgatggc cggggcggtc ctcaccaacc
     3001 tgttcatcct cggcgcgagc ccattgctcc cgatcggttt gctcgtgata ggcgcgcttg
     3061 tggcctgggg ccgctggcca cagaccaggt ctctagtcgc cggttccaga cggcgagtgg
     3121 cgtagtagaa cgcctcgccg tagtcgcacg gaaaggaacg gcagtgatcg aaaaccttca
     3181 gcagcaggtc cagggggcca tcgaagagct ggtcgagtcc ggtaccgagc gcggcctgca
     3241 ggtcgccgtc taccgggaag gcgagcaggt ggtcgacgcg gtcgccggcc tcgccgaccc
     3301 ggagaccggg cgcccggtct cctcggacac gcctttctac gcctactcta cgggcaaggg
     3361 cgcgacctcc gccgtagcgc acgtgctggt cgagcgcggg ctcttcggct acgacacgcc
     3421 tgtttcggag ttgtggccgg agttcgggac ccacggcaag gagggtgcga ccgtgcgcca
     3481 cgtgctgacg cacacggtcg gtgttcccgg catacccgca gacaccatgc ccgaggacct
     3541 atgcgactgg gacggaatgt gcgcggccat agccgactcc gagccctggt gggagccagg
     3601 cacgaagacc gcctaccacg cctacccgtt cggctacatc ctcggggaga tagcccggcg
     3661 ggccacgggc aagcccatct cgcgggtgct gaaggaggaa gtggcgggac cgctcggtgt
     3721 cgccgacgag atctacttcg ggatgccgga atccgagctc taccgcctgg cacctcttga
     3781 ggatgtggag ggcagcgcgg agttcctcgc ctcgatgcct gacgattcgc cgttcttccg
     3841 cttgggtccg cggaccgttg ccccgacggc cgagttcggc aaccgcaagg acatactcat
     3901 ggccgacatc cctgccggtg gcaagatgtc ggccggggcc atagcacgca cctacgccgc
     3961 aatgatcggc gaggtggacg gggtgcggct cgtctccccc gggcggctgc gtgagatctc
     4021 cgcgccggcg ttcgggggta cggacgagat catgggcaac cccgtgacgt gggcgctggg
     4081 ctactccatc ggccggctcg ggagcgacgc gcagcagtcc ccgaccgtgt tcgggatggg
     4141 gggagtaggg ggaagcttcg cctacgccga taccgcgacc ggcgtcgctt tcgcgctgac
     4201 caagaacagg ctcaccccag acttctcagc agcccagcga atctccgaca tggtcacgaa
     4261 agccgtcggg agctaggttg gcatcggacg gaagagcatc gcagatgggc ctgggcaggt
     4321 tcaaggtggt agccgggctt gcggtctccg acatggaccg ggcggtcgaa ttctatgaag
     4381 aaaagctcgg cctcacgagc tggacggggc aggagaacaa tcgggcttat gagtgtggcg
     4441 aaggtaccgt gctgcacgtc tatctctcac cggatcatgc gggtggaact accgccacgc
     4501 tggcgagctg gtacgtccgg gagatagaga gcctggtgga cgttctcacc tccagaggcg
     4561 tggttttcga gagatacgac cggcccccga tccttacgga cgacaggggc atagccacgt
     4621 tcgaaggcgg cgcgaaggtc gcgtacttca aggacccgga cggaaacacg ctctccctgg
     4681 cgcaggttcg ggatcgcagc cctacagatg aggaccgatg agaaagctaa ccgcgttcca
     4741 gttgatgtcg ttggacgggg tcgttgagtc gccgaacgag tgggctttct cgtactcgga
     4801 cgaggagatg gagcgggaga acgcggctgg gatggcggcc tctgatgcgt tgcttctggg
     4861 gagggcaacc tacgaggagt tcgcctcctt ctggccctac cagcccggcg gcacacagat
     4921 ggtggactac ataaacggcg tgcgcaagca cgtggtatcg acgaccctgg aggagcccct
     4981 cgggtggaac aactctaccc tgatcggggg agacgtcgca gaggagataa acgccctgaa
     5041 agagcagccc ggagagaaca tcacgatcac gggcagcatc acgctcgtca ggtggctgat
     5101 aaggaccggc ctcctcgacg agctccggct catggtgtat cccgtggtac tgggcggcgg
     5161 gagacgcctc ttcgaagacg ggggagagcc ggaggtgctg gagctcgcaa gctcgaagac
     5221 gtacgccacg ggcgtcgtct ccctcgccta cagaccatcg ggaggggtga gctcctgaaa
     5281 gttggaaagc gcaagctggt cgtctgcaac gccgtgtccc tggacggttt ctacgagggg
     5341 ccgaacaaga acgtcatgga cctcttcgaa tatcgtcggt cctatccgac ggacgagagc
     5401 ttcgacgact acaacgccga gcgtctgcgc gcggcagaca ccctgctcct cgggcgtacc
     5461 tcgtacgaag ggttcaaggg cttctggccg ccggtggcga atgaccccga cgctaggccg
     5521 cttcagcggg agatctcccg gctcaacaac gccatagaca aggtggtcgt ctcggacggc
     5581 ctgaccgcag gcgagacggc accctggggc aacacccgca tcgtcggccg cgcggatgcc
     5641 cacgagcaga tcgccgagct caaacgccag accggtgggg agatcctggt cttcggcagc
     5701 cgcacgctgt ggaacgactt gctggaggat ggcctcgtcg acgagttgca cctgatgata
     5761 ggacccgtcc tggtgggagc tggcacgccg ctgttcggcg gccggccaac ggtaacgctg
     5821 cggctgctcg gtacccgcac gtgggaaggc tcgggaaacg tgctcgccag gtatgaggta
     5881 agaccgaacg actcgagagg agaaggataa gcatggactt ccggatcgaa gtagtgacgg
     5941 ttccggtaac ggatgtggaa cgatccctcc ggttctacgc ggagaagatg ggctttgcgg
     6001 tggatataga ccacaaggtc agcgacgagg tccgcctcgt ccagctaacg ccccccggct
     6061 ccttctgttc catacacctg ggtcgtggaa ccatcgccac gagacccggc gacctcgacg
     6121 gcgtgttcct ggtcgtcacg gacgtgcgcg ccgcccacgc cgaccttgtg aagaaggacg
     6181 tggacgtggg tgagatccag atcttcgacg acggcgctta ccggccggcc cgcgaagacg
     6241 agaacctcga catggtcggg tgcgtcttct tccgggaccc cgacggcaac cgctggtgca
     6301 tccagcagat accagcccgc tagagggcgg tccgcttcgc gaaccgcgcg gtcagctatc
     6361 agccttcagc cgagctgatg gctagagcaa cgaaagggga aagagcatgg gcaaggtaac
     6421 ttcagggctc acgatgtcgc ttgatgggtt catagccggc ccgaacgacg ggcccgagca
     6481 cccgctcggg gaggacggga tgcgcctctt cgactggtac gggggcggcg atacggagta
     6541 cgtggtgccg agcggcggga tgacgttcca ggtctcctcg cagagtgccg agatgttgcg
     6601 cggggtgttc tcctccatag gggcgatcgt gaccggtcgc agaacgttcg atatcacgaa
     6661 cggctggggc gggaggcatc ccctcgacgt tccggtcgtg gtcctcaccc acaacgttcc
     6721 agatggctgg gaatacgagg gatctccttt caccttcgtc acggacggcg tagagagcgc
     6781 ggtcgagaag gcaaggaagt tggccggcga gaagcacgtg gcggtcggcg cggcgagcct
     6841 cgttcagcag tgtctccggg ccgggttact cgacgaggtg cacgttgacc tcgtgcccgt
     6901 gctgctcggt ggtggggtca ggct
//
LOCUS       DBNGVD010000398         2210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3192162, whole genome shotgun sequence.
ACCESSION   DBNGVD010000398 DBNGVD010000000
VERSION     DBNGVD010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3192162"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC16CM_10790"
     CDS             <1..387
                     /locus_tag="AC16CM_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140361.1"
                     /translation="LYAGAADQIDLTVRDVRALARAALSVVQPNEGSPEPLSEAVRGL
                     ATATGALATYLQTSGDPEDTRRLALEAASEASALLYEREDLASNLAVNALVDQIHSST
                     VDILGSTGMDRSSALQALEDATGRAS"
     gene            complement(757..1602)
                     /locus_tag="AC16CM_10795"
     CDS             complement(757..1602)
                     /locus_tag="AC16CM_10795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3140362.1"
                     /translation="MKFSVLIVNYASWPLTLRCIASLDATGYTDFETIVVDNDSAEPP
                     LLPPGVQLIRNRKNVGFARAHNRGIAASTGDVVVLINPDTVVEENFFDGLEAFLAETP
                     KAGIVGPRVLEADGELQLSARREISALSGLLGRTSLLTHLFPKNSLVKSQFPAVTEGS
                     YPSAVDWVSGACMAIRRETLQGIGPLDERFFMYFEDADLCRRARASGWLVYYVPGVEI
                     VHQTGASSRNKPRAVWLMHKSAFLYHRKHGTHGPLNVLSAAVLAGLTLRALAKLATSL
                     LGRGA"
     gene            complement(1599..>2210)
                     /locus_tag="AC16CM_10800"
     CDS             complement(1599..>2210)
                     /locus_tag="AC16CM_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3140363.1"
                     /translation="DAIRLSGLPYRLGHGERDRGQFEEPGYVFGACAAAALYRGSLFG
                     EIGYFDEDFVSYCEDGDLSFRAQLAGHRCFYVPGAVVYHMGSASTGGKRSPTATRLGT
                     QNSLGLLVKDLPRSAVPHVLPFFVLGQLARLATAAATGSLRAHLEGLLGAWRHLPLMR
                     EKRAVIQKRRRISDAEVRRLLRRSSLAAAASIARRLRDSVVTR"
BASE COUNT          397 a          734 c          720 g          359 t
ORIGIN      
        1 ctctacgccg gggccgccga ccagatcgac ctgacggtcc gcgacgtgcg tgccctcgcc
       61 cgggccgccc tcagcgtggt gcagccgaac gaaggctccc cagaaccgct ctcggaggcg
      121 gtacgcggcc tggcaacggc aaccggggcc ctggcaacct acctgcagac ctccggcgac
      181 cctgaagaca cgcgccggct cgcactggag gccgccagcg aagccagcgc gctcctgtac
      241 gaacgcgagg acctcgccag caacctcgcc gttaacgccc tggtagacca gatccactcc
      301 tccaccgtgg atatcctagg aagcaccggt atggaccgta gctccgcgct ccaggccctg
      361 gaagacgcca ccggccgcgc atcgtagtgg atagagctat cagctgtcag ccgggaacaa
      421 gaagccgaat gcctgcggct ctctccataa acgtcgaatc ctcttcaagt agctgtagac
      481 tctctccacc cggaagtgtt tcgaaggtca gcgatgatac cgaatccggt tatggagtcc
      541 gtcctcggga tgtgggctaa cataccgatc ctccatcacc cctgccttcc tcaccatcat
      601 tcccgcgtcc tctcaccgtc attcccgcga aagcgggaat ccacgtctgc tcgaagacgt
      661 aggcccgcgc gtgccagggg cactattcat ccggattccg taaaaccgcc ccacctgcaa
      721 tagctgaaag cttgataact gacaacgcgt ggtgcttcag gcaccccgcc caagcaacga
      781 ggttgccagc ttggcgaggg cgcgtagggt caggccggcg aggaccgccg cgctcagtac
      841 gttgagtggg ccgtgggtgc cgtgtttgcg gtggtagagg aaggcgctct tgtgcatgag
      901 ccagacggcc ctgggtttgt tgcggctgct cgcgccggtc tggtgaacga tctcgacccc
      961 gggaacgtag tagacgagcc agccggaggc gcgggcgcgg cggcagaggt cggcgtcctc
     1021 gaagtacatg aagaagcgct cgtcaagcgg cccgatcccc tgtagcgtct ccctgcggat
     1081 ggccatgcag gcgccggaga cccagtcgac cgcggaaggg taagatcctt ccgtgaccgc
     1141 ggggaactgg ctcttgacca gcgagttctt cggaaacagg tgggtcagga gcgaggtgcg
     1201 tccgagaagg ccggagaggg cgctgatctc tcgccgcgcc gagagctgaa gctccccgtc
     1261 ggcctccagt accctcggtc cgactatgcc cgccttcggg gtctcggcga gaaacgcctc
     1321 aaggccgtcg aaaaagttct cctccacgac ggtgtccggg tttatgagga ccactacatc
     1381 cccggtcgag gcggcgatgc ccctgttgtg ggcgcgggcg aaccccacgt tcttcctgtt
     1441 ccggatgagc tgcacccctg gtggcagcag cggcggctcg gcgctgtcgt tgtccaccac
     1501 gatggtctcg aaatctgtgt agccggtcgc gtcgagcgac gcgatgcagc gcagggtcag
     1561 cggccaggaa gcgtagttga cgatgagcac ggagaacttc accgggtcac cacactgtcc
     1621 cgaagccggc gcgcgatgct cgccgcggcg gcgagcgaag atctcctgag caacctgcgg
     1681 acctcggcgt cggagatccg cctgcgcttc tgaataaccg cccgcttctc acgcatgagc
     1741 ggaaggtggc gccaggcccc gagcaagcct tccaggtgtg cccgcaatga gcccgtagcg
     1801 gcggccgtgg cgagacgcgc gagctgtccc aggacgaaga acggcagaac gtgcggcacg
     1861 gccgagcggg gcaggtcctt tacgagcaag ccgagggagt tctgtgtgcc gagccgggtc
     1921 gccgtcgggc tgcgcttgcc cccggtggag gcgcttccca tgtggtagac gacggctcct
     1981 gggacgtaga agcaacggtg gccggcgagc tgggctcgga agctgaggtc gccgtcctcg
     2041 cagtaggaga cgaaatcctc gtcgaagtat cctatctccc cgaagagcga cccccggtag
     2101 agcgccgctg ctgcgcacgc cccgaacacg tagccgggct cctcgaactg gcccctgtcg
     2161 cgctcgccgt gccccagccg gtaaggtagc ccgctcaggc gtatggcgtc
//
LOCUS       DBNGVD010000399         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2463884, whole genome shotgun sequence.
ACCESSION   DBNGVD010000399 DBNGVD010000000
VERSION     DBNGVD010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2463884"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /gene="aldA"
                     /locus_tag="AC16CM_10805"
     CDS             <1..1325
                     /gene="aldA"
                     /locus_tag="AC16CM_10805"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007497.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR3140364.1"
                     /translation="KAQRGWARKTGPERGAVLRAVARKIRDRKEELAQLVVAEQGKTI
                     SEARGEVGDAAAGFFDYYATFERAQVGSMFAPDEPGEHLFVRSVPYGVVVGIIPWNYP
                     AALFARKVAPAIMAGNTIVLKPHEDTPLSALALAAIVEEAGVPPGVVNVVTGAGRVVG
                     DALVRHPITEMVSVTGSVRGGREILAAAAENITPVSLELGGKAPFIVLEDADLGAAVE
                     NAVDARFWNCGQVCTCNERTYVQRGVYEEFVERFVEAASSLRMGDPTREDVQMGPKVN
                     EPELTKVEALVREAVGQGANVRLGGGRPEGREFEKGYWFEPTVLTETTNDMDIIQSEV
                     FGPVLPIQPFDDFEEVVGLANDSRYGLTAYVYTSDLHKAMRAIDDIDFGEIYVNKIGP
                     EQIQGFHTGYRLSGMGGDDGPYGYERYLRKKTVYLHYDDFGTPAGLHP"
     gene            1351..>2314
                     /locus_tag="AC16CM_10810"
     CDS             1351..>2314
                     /locus_tag="AC16CM_10810"
                     /EC_number="1.1.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008873 - gluconate 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008875 - gluconate dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HIR3140365.1"
                     /translation="MERQTPALLVLTPYEARTAAAVFERLFPADENGPGATDIGVIAY
                     LDRALAGAYRDQAEAYRLGLSALDLAARRRGGAPFADCSAEHQDALLSDLERGELPDF
                     LVPPQRDFFEMLRAHLQEGLFADPAHGGNREKLGWKLLGHPGVWLENSAEENLSEEPV
                     TKGGRIQSLEDVGYSLNGAPSEPVGIPGYDPQRGAEPPDGVADVVLVGMGAMGSVVAP
                     ILARAGLRVVGLEAGPWRTRHDFLPDELGSSYYCRGEMGPKFLSETPRWRRNEGEPTR
                     EATFSLGRMMNGVGGSVIHWGGALRRMHPHHFRYLTHVRQSFGEG"
BASE COUNT          411 a          732 c          805 g          366 t
ORIGIN      
        1 acaaggccca gcgcggctgg gcgcgcaaga ccggtcccga gcgcggagcg gtgctcaggg
       61 cggtggcccg gaagataagg gatcgcaagg aggaactggc ccaactggtc gtcgcggagc
      121 agggcaagac gatctccgag gcccgcggcg aggtcggcga cgcggccgcc ggtttcttcg
      181 actactacgc caccttcgag cgtgcccagg tgggaagcat gttcgcgccg gatgagcccg
      241 gcgagcacct cttcgtcagg tcggttccct acggcgtggt ggttggcatc atcccgtgga
      301 attacccggc ggcgctcttc gcccggaagg tggcaccggc cataatggcg ggaaatacca
      361 tcgtgctaaa gccgcacgag gacacgcctt tgtcggcgct cgctctcgcc gcgatcgtgg
      421 aggaggccgg ggtacccccc ggcgtcgtca acgtcgtgac gggcgccggc agggtcgtcg
      481 gcgatgcgct ggtgcggcac ccgatcaccg agatggtgag cgtgaccggc tcggtacgcg
      541 gcggacggga gatccttgcc gcggcggcgg agaacatcac ccctgtctcc ctcgagctgg
      601 gagggaaggc accgttcata gtgctggagg acgccgacct gggcgccgcg gtagagaacg
      661 cggttgacgc acggttctgg aactgcgggc aggtctgtac ctgcaacgag cgcacctacg
      721 tgcagcgcgg cgtctacgag gagttcgtcg agcgcttcgt cgaagcggca tcctccctgc
      781 gcatgggcga ccccacgcgc gaggatgtgc agatgggacc caaggttaac gaaccggagc
      841 tgacgaaggt cgaggccctc gtccgggagg ccgtggggca aggggccaac gtacgactcg
      901 gcggaggtcg cccggaaggg agggagttcg aaaaggggta ctggttcgag ccgacggtgc
      961 tcaccgagac cacgaacgac atggacatca tccagagtga ggtcttcggc cccgtgctcc
     1021 cgatacaacc tttcgacgat ttcgaagagg tagtgggcct cgccaacgac tcccggtacg
     1081 gcctgaccgc ctacgtgtat acctccgacc tccacaaggc gatgcgggcc atcgacgaca
     1141 tagacttcgg cgagatctac gtgaacaaga tcgggcccga gcagatccag ggtttccaca
     1201 ccggataccg tctctccggc atgggcggcg acgatgggcc gtacggatac gagcgctatc
     1261 tccgcaagaa gacggtgtac ctgcactacg acgacttcgg cacgcccgca gggctccatc
     1321 cctagccgat ccgaagacct ggagaggtag atggagaggc aaacaccggc cctgttggtt
     1381 cttacaccgt atgaggcaag gacggcggcg gccgtcttcg aacggctctt tccggccgat
     1441 gagaacggtc ccggagctac agatataggc gtcatagctt acctggaccg cgcgctcgct
     1501 ggtgcctatc gggaccaggc cgaagcctac cgtctcggcc tgtcggcgct cgacctcgcc
     1561 gcccgtcggc gcggcggggc gccttttgca gactgttcgg cggagcatca ggatgctctt
     1621 ctgtccgacc tggagcgggg cgaactccca gactttctcg tccccccgca gcgcgacttc
     1681 ttcgagatgt tgcgggccca cctgcaagag ggtctcttcg ccgatcccgc ccacggaggg
     1741 aaccgggaaa agctgggctg gaagcttctg ggtcacccgg gggtctggct cgaaaactcc
     1801 gccgaggaga acctctccga ggaacccgta accaagggcg gtaggatcca gtcgctcgaa
     1861 gacgtcggtt actccctgaa cggtgccccg agcgaacccg tcggcatacc cggctacgac
     1921 ccgcagcggg gcgccgagcc tccggacgga gtggctgacg tggtcctggt ggggatgggg
     1981 gcgatgggca gcgtggtggc ccccatcctg gcacgtgcgg gactgcgcgt ggttggcctt
     2041 gaagccggcc cgtggaggac caggcacgat ttcttgccgg acgagcttgg gtcctcctac
     2101 tattgccggg gagagatggg gcccaagttc ttgagcgaga cgccccgctg gcgccgcaac
     2161 gagggcgaac cgaccaggga ggccaccttc tcgctggggc gcatgatgaa cggcgtgggc
     2221 ggctcggtca tccactgggg cggcgcgctc aggcgcatgc acccccacca cttccgctac
     2281 ctcacccacg tacgccagag cttcggcgag gggg
//
LOCUS       DBNGVD010000400         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3921106, whole genome shotgun sequence.
ACCESSION   DBNGVD010000400 DBNGVD010000000
VERSION     DBNGVD010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3921106"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="AC16CM_10815"
     CDS             complement(<1..1895)
                     /locus_tag="AC16CM_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR3140366.1"
                     /translation="MLVKDELLDKIVARIKEQMPEDQAPQVEEFVRQYYGWVDTDDLE
                     DLSPVDAYGAAVSHWAFAGRREPGERKIRIYNPVFEEHGWQSTHTVVEMINDDMPFLV
                     DSTRMEVNRQGYAIHMILHPLIKVRRDEEGRLIEVLPPDAEDEDAISESVIHVEVDRQ
                     TEPEVLEELKAAIEKTLADVQASVEDWLEMRDQVGGIVTGLEENPPGFEQEDLAETRA
                     FLEWVEDNNFTFLGYREYDLTTLDGEDALRAVEGSGLGILRQTERGAVSHSFAELPPE
                     VRRLARTPKLLNLTKANSRATVHRPSYLDYIGIKKFDESGEVAGERRFLGLYTFTAYS
                     SSVFDIPLVRRKVRYVLERSGFPEGSHNEKDLVEILETYPRDELFQMSKEELFEIAMG
                     VLHLQERQRVKLFVRRDTYGRFFSCLVVVPRDRYNTLIRERMQDILLWAFDGENVEYN
                     VRLSESVLARIHFIIYTKPGDAPDYDGEDIERRLVETTRSWADSLYEALLEQCGEERG
                     TELFHKYRDAFSPGYRAGFMPRTAVSDIVRMETLDSDDDLAMSLYHPIEEPEDFLGFK
                     LFRLGDQVSLSGILPLLEDMGVEVVDERPHEIKPDGSPPIWIYDFGLVHGANGELQTS
                     EVKEIFQ"
     gene            complement(2088..>3403)
                     /locus_tag="AC16CM_10820"
     CDS             complement(2088..>3403)
                     /locus_tag="AC16CM_10820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR3140367.1"
                     /translation="TQTRYQQYVGDAAVYGGQITVLSRDGQRAAVVGNHYTDLTPSNQ
                     VELSKAGAREKVQRVRGNSGNFDTTLYINPKNGIRFYEVDSRRFDSRKVFHINAQTGS
                     TIRSYDNLQEATETGIGVNRDTKQLDVTVSGTSHQLVSTDKRQATYDARNTKRLPGTL
                     FLDPNSDGLWNLAGRTSPGQPAGVDAHYHANVADDFYRAVFGRNSLDNKGMQMVSTAH
                     YSRNYNNAFWNGRQVTYGDGDAKVFIELSGGLDVATHEFTHGVTEFTSGLIYQGESGA
                     LNESFSDMMGNSSEYWAADRPDWQIGEEVYVATTTVPGFRNMADPAEDGDPDHYTERY
                     TGTGDNGGVHTNSGIPNHAYYLVVNGGRNAGEARGHAHTGPSVTGIGLAAAERIFYDG
                     FTSLPSNATMSQARAATVQAANARYGAGSQQATSTADAWTAVGVR"
BASE COUNT          533 a         1087 c         1100 g          683 t
ORIGIN      
        1 tcctggaaga tctccttcac ctccgaggtc tgcagctcgc cgttcgcccc gtgcaccagt
       61 ccgaagtcgt agatccagat cgggggcgag ccatctggct ttatctcgtg cgggcgctcg
      121 tccaccacct cgacgcccat gtcctcgagc aggggcagga tgcccgagag tgagacctga
      181 tccccgagcc tgaacagctt gaacccgagg aagtcctcgg gctcttctat gggatggtag
      241 aggctcatgg cgaggtcgtc gtcggagtcg agcgtctcca tacgcacgat atcggagacg
      301 gcggttcggg gcatgaagcc ggcccggtag ccaggggaga aggcgtcgcg gtacttgtgg
      361 aacagctccg tgccccgctc ctccccgcac tgctccagga gggcctcata gaggctgtcc
      421 gcccacgagc gggtcgtctc cacgagacgg cgttctatgt cctccccgtc gtaatcgggg
      481 gcatcccccg gcttcgtgta gatgatgaag tggatgcggg ccagcacgga ctccgagagc
      541 ctgacgttgt actccacgtt ctccccgtcg aaggcccaca gtagtatgtc ctgcatgcgc
      601 tcacggatca gggtgttgta ccggtcgcgc ggcacgacga cgaggcagga gaagaagcga
      661 ccgtaggtgt cgcgcctgac gaagagcttg acgcgctgac gctcttgcag gtgcaggacg
      721 cccatcgcga tctcgaaaag ctcctctttg gacatctgga acagctcgtc gcgcgggtag
      781 gtctccagga tctccacgag gtccttctcg ttgtggctcc cctcgggaaa accagacctc
      841 tcgagaacgt agcgcacctt gcgcctgacg agtgggatgt cgaagacgct ggagctgtag
      901 gcggtgaagg tgtagagccc caggaaccgc cgctcgcccg cgacctcccc cgactcgtcg
      961 aacttcttga tgcctatgta gtcgaggtac gatggcctgt gcaccgtggc ccgcgagttg
     1021 gccttggtga ggttgaggag cttgggcgtg cgggccagcc tcctcacctc gggcgggagc
     1081 tcggcaaagc tgtgggagac ggctcctcgt tcggtctgcc tcaggatacc gaggccagac
     1141 ccctcgacgg cgcgcagggc gtcctcgccg tccagcgtgg tcaggtcgta ctcgcggtac
     1201 ccgagaaagg taaagttgtt gtcctccacc cactccagga aagcgcgggt ctccgccaga
     1261 tcctcctgct cgaagcccgg cgggttctcc tcgaggccgg tgacgatccc gcccacctgg
     1321 tcacgcatct ccagccagtc ctccaccgac gcctgcacgt cggcgagggt cttctcgatc
     1381 gccgctttca gctcctcgag aacctccggc tccgtctggc ggtccacctc gacgtggatc
     1441 accgactccg agatggcgtc ttcgtcctcc gcgtcggggg ggagtacctc gatgagccgc
     1501 ccctcctcgt cgcggcgcac cttgatgagg gggtgcagta tcatgtggat ggcgtagccc
     1561 tggcggttta cctccatcct cgtcgaatcc acgaggaagg gcatgtcgtc gttgatcatc
     1621 tccaccacgg tgtgggtcga ctgccagccg tgctcctcga agaccgggtt gtagatcctg
     1681 atctttcgct cccccggctc gcgcctgccc gcgaaggccc agtgggagac ggcggccccg
     1741 taggcgtcta cgggagagag gtcttccagg tcgtccgtgt caacccagcc gtagtactgg
     1801 cgcacgaact cctcgacctg aggtgcctga tcctctggca tctgctcctt gattcgcgcc
     1861 acgatcttgt ccagaagctc gtcctttacc agcataagca agcctcccta aaaattccca
     1921 gctttcccag ttacccgcac cgcccattat agtcccgcag taatctccct ggggagcatg
     1981 aagaacgggg ctctcctcta aggaaagccc cgctactctt gtacgctggt ttttacctca
     2041 gccccaggct ttcggcctaa aagctgacgg ctgacagcta gatagctcta ccgtacgccc
     2101 acggcggtcc aggcgtcggc ggtggaggtc gcctgctgcg agccagcacc gtaacgggcg
     2161 ttggccgcct gcacggtcgc cgcgcgtgcc tgggacatgg tggcgttcga tggcaggctc
     2221 gtgaagccgt cgtagaagat gcgctccgcg gcggcgagcc cgatgcctgt tacggacggc
     2281 cccgtgtggg catgtccacg cgcttccccg gcgttgcggc cgccgttgac cacgaggtag
     2341 taggcgtggt tggggatgcc gctgttggtg tgcacgccgc cgttgtcccc cgtgccggtg
     2401 tagcgttcgg tgtagtggtc cgggtccccg tcctcggccg ggtcggccat gttgcggaaa
     2461 ccagggacgg tcgtggtggc aacgtagact tcctcaccga tctgccagtc tggcctgtcg
     2521 gcggcccagt actcgctgga gttgcccatc atgtccgaga aggactcgtt gagcgcgccg
     2581 gactcgccct ggtagatgag gcccgaggtg aactcggtga cgccgtgggt gaactcgtgg
     2641 gtggccacgt cgagcccgcc cgagagctcg atgaagacct tggcatcgcc gtcgccgtag
     2701 gtgacctgcc ggccgttcca gaaggcgttg ttgtagtttc tcgagtagtg ggcggtcgag
     2761 accatctgca tgcccttgtt gtcgaggctg ttacggccga aaacagcccg gtagaaatca
     2821 tccgcgacgt tcgcgtggta gtgagcgtcc acgccagccg gctgccccgg cgaggtgcgt
     2881 cccgcgaggt tccagaggcc gtcactgttg gggtcgagga agagcgtgcc tggcagcctc
     2941 ttcgtgttcc gcgcgtcgta ggtagcctgg cgcttgtccg tggagaccaa ctggtgcgag
     3001 gtgccgctga cggttacatc cagctgcttg gtgtcccggt tgacgccgat gcccgtctcg
     3061 gtcgcctcct ggaggttgtc gtaggaccgg atcgtggagc ccgtctgggc gttgatgtgg
     3121 aagaccttgc gcgagtcgaa gcgcctggaa tcaacctcgt agaacctgat cccgttcttg
     3181 gggtttatgt agagggtcgt gtcgaagttg ccgctgttgc ccctcacacg ctgcaccttc
     3241 tcgcgcgccc ctgccttcga gagctcgacc tggttcgagg gggtcaggtc ggtgtagtga
     3301 ttgccgacca cggcggcccg ctgcccgtcc cggctcagga ccgtgatctg gcccccgtaa
     3361 accgcggcgt caccgacgta ctgctggtat cgggtctgcg tga
//
LOCUS       DBNGVD010000401         2235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3556754, whole genome shotgun sequence.
ACCESSION   DBNGVD010000401 DBNGVD010000000
VERSION     DBNGVD010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3556754"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16CM_10825"
     CDS             complement(<1..425)
                     /locus_tag="AC16CM_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140368.1"
                     /translation="MNLDLTGRSALVTGAASGIGRACALRLSQAGAAVTVLDLDGGAA
                     GEVAESIGGEALQADLSDYGVLDGLEVEADIIVNNAGMQHVAAVEEFPPERFSMIVRI
                     MLEAPFRIVQKALPRMYEEGWGRIINISSIHGQVASPYKS"
     gene            complement(551..1945)
                     /locus_tag="AC16CM_10830"
     CDS             complement(551..1945)
                     /locus_tag="AC16CM_10830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3140369.1"
                     /translation="MRSSSGTDEAPLVVVGAGACGMVAALAAAKRGTRTLLLEKGTET
                     GGNTARSTGLIPAAGTRFQRRAGVLDDSPRLMAEDILEKNDHESDPALTHLLCEESGP
                     LVEWLVDEVGCEMVCYKDFLYPGQSRHRMHGPRESYGIELVRQLETAVLEEPLITMHT
                     DTPVSGLISSDGRVTGVETAEGTIRAGAVTLALNGFGGDEGMVKSYLGPEAASALYFG
                     SPGNTGEGIRWGMDLGAATDQMASYQGHASVAAPDGPLVTWGLVVNGAILVNRLGRRF
                     GCETVGYSEYAGPVLSQPGGEAWEIFDARVYDASLGTRFDEVVEAGKVTSSETLEDLA
                     GLLGLPADALAETVAETNRSARGESPDAFDRTEFQGGPFGPPYYGIHVRGALFHTQGG
                     LRVDGRARVLRTDNTPIPGLYAGGGTAVGVSGSGYRGYSSGNGLLTATVLGKIAGEEA
                     SGNRQQASGLFPDA"
     gene            complement(1952..>2235)
                     /locus_tag="AC16CM_10835"
     CDS             complement(1952..>2235)
                     /locus_tag="AC16CM_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3140370.1"
                     /translation="LVATNPARAYGLAPRKGSIAVGADADLAIVDMDETHPVTTERLL
                     SAQEYTPFEGMELTGWPVRTILRGETVFANGEPVGKPSGTYLKRPLDIP"
BASE COUNT          359 a          741 c          728 g          407 t
ORIGIN      
        1 gatttgtagg gtgaggctac ctgaccgtga atggaggaga tgttgatgat gcgcccccaa
       61 ccctcctcgt acatgcgggg cagggccttc tgcacgatcc tgaacggcgc ctccagcatg
      121 attctgacga tcatcgagaa acgctccggg gggaactcct cgacggcggc gacgtgctgc
      181 atgccggcgt tgttgacgat gatatcggcc tccacctcca ggccatcgag caccccgtag
      241 tcggagagat cagcctgcag ggcctcgcca ccgatgctct cggccacttc tcccgcggcc
      301 ccgccgtcga ggtcgagcac ggtcaccgcg gctccggcct gcgagagcct cagggcgcag
      361 gcgcggccga tgccgctcgc ggcccccgtc acgagggcgc ttcgccccgt gagatccaag
      421 ttcacagaag accttcccgg taccagagaa gcctcgttca tgttcgtcct tcccgccgag
      481 ttcccgtaca agagattctt ccccagaacc cgaccataaa atcagtatga gtcagataag
      541 gttacaggtt tcaggcatca ggaaaaagac ctgacgcctg ttgcctgttg cctgatgcct
      601 cttcgcccgc gatctttcct agcacggttg cggtcaggag gccgttgccg ctggagtagc
      661 ccctgtatcc gctaccgctt acgccgacgg ccgttccgcc acctgcgtaa aggccgggta
      721 tgggcgtgtt gtcggtgcga agtaccctgg cgcgcccgtc cacacgtaat cctccctgcg
      781 tgtggaagag cgcgccacgc acgtggattc cgtagtaggg tggcccgaag gggccgcctt
      841 gaaactccgt gcggtcgaag gcgtccgggg attcgccgcg ggcggatctg ttcgtttcgg
      901 cgaccgtctc ggccagggcg tcggcgggga gacccagcag cccggcgaga tcctccaggg
      961 tctccgaact ggtgaccttg ccggcctcta ctacctcgtc gaagcgggtg ccgagcgagg
     1021 catcgtacac tctggcgtcg aagatctccc aggcctcccc gcccggctgg gacagtacgg
     1081 ggccagcgta ctccgagtag ccgacggtct cgcacccgaa ccggcgcccc agcctgttga
     1141 cgaggatggc gccgttcacg acgaggcccc acgtgacgag cggtccatcg ggggcggcga
     1201 cggaggcgtg cccctggtag gaggccatct gatccgtggc cgcgccaagg tccatgcccc
     1261 accttatccc ctcccccgtg ttgcccgggg aaccgaagta gagcgcggag gcggcctcgg
     1321 ggccgagata gctcttcacc atgccctcat ctccgccgaa gccgttgagc gctagggtga
     1381 ccgcgccggc tcttatcgta ccttccgccg tctccacacc ggtgacgcgg ccgtccgatg
     1441 agatgagccc gcttaccggg gtatccgtgt gcatggtgat caggggttcc tcgaggacgg
     1501 ccgtttcgag ttgcctgacc agctcgattc cgtagctttc cctggggccg tgcatcctgt
     1561 ggcgactctg tccgggatag agaaaatcct tgtagcagac catctcgcaa cccacctcat
     1621 ctacgagcca ttccacgagc gggccagact cctcgcagag gaggtgcgtc agcgcgggat
     1681 cactctcgtg gtcgttcttc tcgaggatat cttcggccat cagccggggg gagtcgtcca
     1741 ggacgccggc cctgcgctgg aagcgggttc cagcggcggg gatcaggccc gtggagcgag
     1801 cggtgttgcc cccagtctcg gtcccctttt cgagaagcaa ggttcttgtt ccccgcttcg
     1861 ccgcggcgag ggcggcgacc atgccgcatg caccggcccc gaccaccacg aggggggctt
     1921 catcggtacc gctggagctt ctcacagaac tttacggaat atcgagcggg cgcttgaggt
     1981 aggttccgga gggcttcccc acgggctcgc cgttggcgaa gacggtctcg ccgcgcagga
     2041 tggtacgcac gggccagccg gtcagctcca tgccctcgaa cggcgtgtac tcctgggccg
     2101 agaggagacg ctccgtggtt accgggtggg tctcatccat gtccacgatg gccaggtcgg
     2161 cgtcggcgcc gacggcgatg gatcccttgc ggggcgccag gccgtaggca cgggcggggt
     2221 tcgtcgcgac gaggt
//
LOCUS       DBNGVD010000402         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2099893, whole genome shotgun sequence.
ACCESSION   DBNGVD010000402 DBNGVD010000000
VERSION     DBNGVD010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2099893"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC16CM_10840"
     CDS             <1..642
                     /locus_tag="AC16CM_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR3140371.1"
                     /translation="IAGEVAVKHDTCVVFAGLDEGVLESLAETFRTDYYHVWTSTDFV
                     GCEVCAAVKNCYAFGGGFMDGILDREGKTEARYRRYNYGAALFGQATRELEQWMELLE
                     GRPETPRGLPGVGDMFVTTMGGRNVKAGRFAGAGVPFSEVRNDKMEGVTLEGVAAIAV
                     VGEAVERMTERGILAAEDFPLMRHLYDVVARDEPVDMPWDTFFGGEPVPGRVV"
     gene            838..1395
                     /gene="efp"
                     /locus_tag="AC16CM_10845"
     CDS             838..1395
                     /gene="efp"
                     /locus_tag="AC16CM_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3140372.1"
                     /translation="MISTNQFRNGSAIRVDGKRFTILFFQHVKPGKGGAFVRTRLRNI
                     DSGAVVEKTFRAGEKLENVRTESRQMTYLYRDGDLLYFMDSDTYEQSPVPEEVVGEAA
                     GYIVEGGTVIILSADGEVVSVEPPPHVDLEVSETDPGLKGDTATGGNKPATLETGVVV
                     QVPLFVNVGDRVRVDTRSNEYLTRV"
     gene            1430..>1707
                     /locus_tag="AC16CM_10850"
     CDS             1430..>1707
                     /locus_tag="AC16CM_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3140373.1"
                     /translation="MSRRTARKNAFLNLYQSDVNHASLDSTIGRWREYRGDLEPYALR
                     LVRGVQKEQEGLDAKLAEVSEGWPLHRMSAIDRTILRLALYEMLYVDDV"
BASE COUNT          317 a          499 c          597 g          294 t
ORIGIN      
        1 atcgccgggg aggtcgccgt aaagcacgac acgtgcgtgg tcttcgccgg gctcgacgag
       61 ggggttctgg agagcctcgc ggaaacgttt cgcacggatt actatcacgt gtggacctcc
      121 acggactttg tcgggtgcga ggtctgcgca gctgtcaaga actgctatgc gttcggcggg
      181 ggcttcatgg acggcatcct ggacagggag ggcaagacgg aggctcgcta ccgccgctat
      241 aactacgggg ctgccctgtt cggccaggcg acgcgggagc tcgagcagtg gatggaacta
      301 ctcgaaggcc ggccggagac gccgcgcggc ctgcccgggg tgggggacat gttcgtcacc
      361 accatgggtg ggcgcaacgt gaaggccggc cgctttgcag gggccggcgt gccgttttcg
      421 gaggtgcgga acgacaagat ggaaggcgtt acgctcgaag gcgtggcggc catcgcggtc
      481 gtgggcgagg ccgtggagag gatgaccgag cgcggcattc tcgcggcgga agactttcct
      541 ctcatgcgcc acctctacga cgtcgttgcc agggacgaac ccgtggatat gccctgggac
      601 accttcttcg ggggtgagcc ggtcccgggc cgcgtcgtct agggcagcag gaaggttaca
      661 ggcttcaggt gtttctctga agcctgtaac ctgatacctg cttgagatca ggctttcatc
      721 ccccgaaggc ggctgacagg cacggaaact gtgcgataat agcccggcgt ctgcgccggg
      781 tttgcgccgc gcaggatcgt tgcgaagatc gggcgccgtg aaggggagct ttcgagcatg
      841 atatctacca accagttcag aaacggctcg gccattcggg tcgacgggaa acggttcacg
      901 atactgtttt tccagcacgt caagcccggc aagggcgggg cgttcgtcag gacgcgcttg
      961 cggaacatcg acagtggggc cgtggtcgag aagacattcc gcgccggtga gaagctggag
     1021 aacgtcagga ccgagtcacg gcagatgacc tacctctaca gagatggaga cctgctctat
     1081 ttcatggact cggacaccta cgagcagtcg ccggttcccg aggaagtggt cggcgaggct
     1141 gcgggctaca tcgttgaggg cggcaccgtg atcatcctgt cggccgacgg cgaggtggtc
     1201 agcgtggagc cgccgccgca cgtggacctc gaggtctccg agaccgaccc cggcctcaag
     1261 ggcgacacgg cgacgggcgg gaacaagccg gcgacgctcg agacgggcgt cgtcgttcag
     1321 gtgccgctct tcgtcaacgt cggcgaccgg gtacgagtgg acacccgctc caacgagtac
     1381 ctgacgaggg tatgacgccg gtgaacccga gcctcgtttc tggcctcgca tgagccgaag
     1441 gacggcccgc aagaacgctt tcttgaacct gtaccagagc gacgtcaacc acgcttccct
     1501 tgactcgacc atcgggcgct ggcgggagta caggggggac ctcgagccct acgccctcag
     1561 gctcgtccgg ggcgtgcaga aggagcagga agggctggat gccaagctcg cagaggtctc
     1621 tgaagggtgg ccccttcacc gcatgagcgc catagaccga accatcctgc gcctcgcgct
     1681 ctacgagatg ctctacgtag atgatgt
//
LOCUS       DBNGVD010000403        10507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4832089, whole genome shotgun sequence.
ACCESSION   DBNGVD010000403 DBNGVD010000000
VERSION     DBNGVD010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4832089"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="AC16CM_10855"
     CDS             <1..1270
                     /locus_tag="AC16CM_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3140374.1"
                     /translation="RPEDRTPVPVPYNPDTEREHSGGSMNFGLSQEQREIKDRAARFA
                     DCEVAPHAADLDGEDRVPFETLAKMSGEGFMGLCVPEEYGGGGADFVSYCLLLEEVSR
                     ADAGLGATLAVHTSAGTLPILLFGTEEQKARWVPSLARGEKIGCFCLTEPETGSDASA
                     ITTRAERVDGGYRVSGHKQWVTNGRIAGTAVLFARAEGGDSEGVTAFVVDLEAEGISF
                     GAHAKKMGVISATTDDVLFDDVFVPERDRLGEEGKGLRVALGTLDSGRIGIAAQAVGI
                     AEAAFRYATRYAAERTTFGKPIAEHQAIAFKLADMQTGIKAARLLVYEAAWIKDRGER
                     HTEAGARAKVFASQVANEVTYEAVQVLGGRGYMKDHPVERHYRDARVTEIYEGTSEIQ
                     RLVISRAIMREHAESLEAPGGVEGSHPVVG"
     gene            1275..2468
                     /locus_tag="AC16CM_10860"
     CDS             1275..2468
                     /locus_tag="AC16CM_10860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3140375.1"
                     /translation="MRRAIRSLVTLLAVFGSLAWIVAVAGWLRGARRVPVLRETAEAK
                     GPIGRYSSVSVVVAARDEEAGVGEALWSVLDQDYPGELEVLAVDDRSTDRTAEIIAGL
                     AALRPGRLRMLKVDRLPDGWLGKNHALYRGAAEACGEWLLFTDADVRFSPDCLELAMR
                     YAEHKRLDHLTLAPELLSHGVALKSFVTAFTFVFGVTQRPWRAADPTAREAVGVGAFN
                     LLKREAYGRAGTHEAIPLRPDDDMRLAKLLKEAGYSQDVAYGTGCVSVEWHQTLGGAI
                     RGLEKSIFPGVDYRLSAIIAASLLLFLTNVWPFAGVFLARRRAMRLLYGADVLAIVAM
                     YAHGPKVSGYSLSPLYLPLHPFGMAAFIYAALRSAYVALVRGGIQWRGTFYPLRVLKG
                     DDRRG"
     gene            2566..4209
                     /locus_tag="AC16CM_10865"
     CDS             2566..4209
                     /locus_tag="AC16CM_10865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3140376.1"
                     /translation="MDTRKGAKDRWEEAYARGSEREAEFSTMSGVPIKPLYTPDDVEG
                     EYDDKLGYPGEYPYTRGVYPNMYRGRLWTVRQFAGYGTAAETNERFRYLLDHGQNGLS
                     VAFDMPTLMGLDSDSPLSLGEVGTEGVAVDSLEDMERLFEGIPMGEVSTSMTINAPAM
                     VLLAMYVVAAEKQGVAAEDLAGTNQNDILKEYIAQKEWLFPPEPSMRVFRDMLVYATE
                     HMPKWNTVSISGYHIREAGSTAAQELAFTLADGFAYVEAGIEAGLDVDDFAPRFSFFF
                     NSHIDFFEEICKFRAARRIWATVLKERYGARDERSLRMRFHTQTAGVSLTAQEPYNNV
                     ARTAFEALAAVLGGTQSLHTNSLDEALALPTEEAVRIAVRTQQIAALETGVANTIDPL
                     GGSYFVEALTDELERQAYHYFEQIDEMGGMIRAIEQGFPMREVSEASARYQRELEENR
                     RHMVNVNIYEPQDEQDMELHRIDPEVSKGQVERLERLKQTRDYDLVEKRLVELREACQ
                     GEGNTMYPILDAVRAYATVGEISATMQEVFGDYRETPVI"
     gene            complement(4258..5100)
                     /locus_tag="AC16CM_10870"
     CDS             complement(4258..5100)
                     /locus_tag="AC16CM_10870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3140377.1"
                     /translation="MTREAVSHASIGQNLRVPVVYHIPVCPFSQRLEILLELKGLGGH
                     VGFHVVDITRPRPGWLLEKTHGTTALPVLDTGEGEVIKESMVILQYLEDRFPEPAVAR
                     RDPYLHAVEGMLIAMEGSFSAQGYRYVMNQDPGRRSELRQGMLDLYARLDEFLVRHNP
                     SGTFLFEEFGLAETVYTPFFMRFWFLEYYEDFDLPQDDRYARVRKWREACLAHPAAGQ
                     VSREQIVKLYYDYAKGMGNGALPPDRTVSSFVFDPDWASRPLPPRDKYGHDATDQELG
                     LAAR"
     gene            5266..6270
                     /locus_tag="AC16CM_10875"
     CDS             5266..6270
                     /locus_tag="AC16CM_10875"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3140378.1"
                     /translation="MAIRETSVRHEALGLDEDDLRRMHYYMLLARKVDERSWILNRQG
                     KAAFVISCQGQEGCQVGAAFNMRPGYDYVYPYYRDHAIVTTLGVSARDELLALLGRKE
                     DPNSGGRQMPGHFSSRELNIVTASAPIGVQYPQAVGTALAFKMRGEDGVVLACGGEAS
                     TSQGDWHEAMNLAGIHDLPVVFMIENNVYAISVPSELQVAGSIARRAEGYGFPGHEVD
                     GNDVLAVYEVAKEAFERARAGEGPTLIEAKTYRMTAHSSDDDDRRYREREEIEEWRQK
                     DPIDRFEGYLLEHGILDEAGRDEISERIRAEVAEASQYAENAPPAGPEDALTGVYAEA
                     "
     gene            6273..7259
                     /locus_tag="AC16CM_10880"
     CDS             6273..7259
                     /locus_tag="AC16CM_10880"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3140379.1"
                     /translation="MTTMNLLQAIHEGLAEEMRADESVMVLGEDVGRAGGVFRVTQGL
                     QEEFGSARVMDTPLAESLIVGAAIGLSVNGMRPVAEIQFGDFIPPAFDQIVNEAAKFY
                     YRSNGAWNVPLTIRAPYGVVPGGALYHSQSIEAWFCNTPGLKVVAPTSPADAKAMLKA
                     SIRDPNPVLFLEHKRAYRLIKEEVPDDGPEVPLGKARVHREGTDITVVSYGLMLYTVL
                     EVAERISEEHGVETEVVEPRTLYPMDTETILDSARKTGKVLVVSEANLTGSLSGEIAA
                     IVAREAFEWLDGPVMRLGAPDVPAAPFARSMLDGFLPSAEEIEGAMMELARY"
     gene            7261..8031
                     /locus_tag="AC16CM_10885"
     CDS             7261..8031
                     /locus_tag="AC16CM_10885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140380.1"
                     /translation="MDVPGYNREAWDRQVESGNPWTVPVGPEVIRAARRGEWEVLLTE
                     TKPVPRAWFPEMAGADVLCLASGGGQQAPIFAAAGARVTVLDNSPGQLAQDRFVAERE
                     ALDLETLQGDMRDLSAFADESFDLVFHPVSNLFVPEVRPVWKEAFRVLRSGGALLAGF
                     LNPVVYVFDLELADDTGEVRVRYSLPYADATSKSVEDLQQQMERGEPLEFSHTLEEQI
                     GGQVEAGFLISGLYEDRHRDDPIAAYMPTYVATRAIKP"
     gene            8046..9341
                     /locus_tag="AC16CM_10890"
     CDS             8046..9341
                     /locus_tag="AC16CM_10890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3140381.1"
                     /translation="MAKPVTMPQLGESVTEGTITRWLKAEGEVVEIDEPLAEVDTDKV
                     NAELPSPVAGKIESLLVPEGTTVDVGTEIVLVADDDAPRKERVALADEFPTAETVARP
                     AAEELDPVALAPRSEGNGRGEVKDAATLRRTRSSPVVRRLAAEHDVKIADIPGSGTGG
                     RVTRKDIEGYIERSGAARGSVTEGPMLQRIEGEERAAVYEGDGIQDLTSIRRAIANRM
                     ATSKREAPHAWTMVEVDVSGLVALREREKAAFVEREGVKLTYLPFIVRAVVESLRENP
                     MLNSVWDGDRIILRKRINIGVAVDLEDALIVPVIPDADELNITGLARRIDALVKRARA
                     GELGTNDVSGGTFTVNNPGSLGSVVSTPIINHPQAAILSAEAIVKRPVVIGDAIAIRS
                     MMNLEVSFDHRILDGGAALRFLNAVKGRLEAYRPEGGLG"
     gene            9369..9758
                     /locus_tag="AC16CM_10895"
     CDS             9369..9758
                     /locus_tag="AC16CM_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3140382.1"
                     /translation="MVQQQSASVETLEAITDAFNAHDLDAIMEFFADDCSLDMPRGPE
                     PWGQRFMGKEAVRAALATRFEGLPDVHYGDGRHWVCGNMGVSEWTLTGTMADGARVRV
                     RGCDHWEFSDGKVIRKDSYWKIVENPA"
     gene            complement(9840..>10507)
                     /locus_tag="AC16CM_10900"
     CDS             complement(9840..>10507)
                     /locus_tag="AC16CM_10900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3140383.1"
                     /translation="QTPALKRHEISHYDERGPAWAWSWLELGEHTGTHLDAPVHWISG
                     RDGNDVSQVPPRHLVAPAVVIDRSTETAEDPDYLLTIDDVRAFEEDHGPLPEGGWLLL
                     HTGWDARAKDPEAFLNARDGNPHSPGFDAECVRWIAEESPLVGVGVETVGIDAGAAGG
                     FDPPFPAHYYLMGANKYGVTQLANLGALPPTGALIIVSPLKLTGGTGSPVRALALVAG
                     SVV"
BASE COUNT         1931 a         3383 c         3507 g         1686 t
ORIGIN      
        1 gcgaccagag gaccggaccc cggtccctgt tccctataat ccggacacag agcgcgagca
       61 ttcgggaggt tccatgaact tcggcctgag ccaggaacag cgagagatca aggatcgcgc
      121 ggcacggttc gccgactgcg aggtggcgcc ccacgcggcc gacctcgacg gggaggacag
      181 ggtacccttc gagacgctcg cgaagatgtc gggagagggc ttcatgggtt tgtgcgtgcc
      241 cgaagagtac ggtgggggcg gcgcggattt cgtctcttac tgcctgctcc tcgaagaggt
      301 gagccgcgcc gacgccggcc tcggggccac gctcgcggtc catacgagcg ctggcaccct
      361 gccaatcctc ttgttcggaa cggaggagca gaaagcccgc tgggtccctt cgctggcgcg
      421 gggggagaag atcggctgct tctgcctcac cgagccggag acgggctccg acgcctcggc
      481 gatcacgacg agggccgaga gggtcgacgg cggctaccgc gtctccgggc acaagcagtg
      541 ggtcaccaat gggcggatcg ccgggaccgc tgtcctcttc gcccgtgcgg agggtggaga
      601 cagcgagggg gtcacggcct tcgtcgtcga cctggaggcg gaaggcatct ccttcggggc
      661 gcatgcgaag aagatgggtg ttatctccgc caccaccgac gacgttctct tcgacgacgt
      721 gttcgttccc gagagagacc gtctcggtga ggaaggtaag ggtctccgtg tcgcgctcgg
      781 cacgctagat tcggggagga tcgggatagc ggcccaggcc gtcggcatcg ccgaggccgc
      841 cttccgttac gccacgcgct acgccgccga gcgaaccacg ttcggtaagc ccatcgccga
      901 gcaccaggcc atagcgttca agctggccga catgcagacg gggataaagg cggcccgcct
      961 cctcgtctac gaggcagcct ggatcaagga tcgtggggag cgccacaccg aggccggcgc
     1021 ccgcgcgaag gtcttcgcct cgcaggtcgc caacgaggtc acctacgagg ccgttcaggt
     1081 cctcggcgga cgggggtaca tgaaagatca tccagtcgag aggcactaca gggacgccag
     1141 ggtcacggag atctacgagg ggacgagcga gatccaacgc ctcgtcatct cccgcgccat
     1201 catgagggaa catgccgaat cgctcgaggc cccgggcggc gtggaaggtt cgcatcctgt
     1261 tgtcggctag gctcatgagg agggccatcc gttctctggt aaccctgctc gccgtgttcg
     1321 gctctctggc gtggatcgtc gcggtcgccg ggtggttaag gggcgcgcgc cgcgtcccgg
     1381 tgctccgcga gacggcggag gcaaaggggc ccatcggtcg ttactcgtct gtctcggtcg
     1441 tcgtggccgc gcgggatgag gaggccgggg tgggggaggc gctctggtcg gtcctcgacc
     1501 aggactatcc cggtgaactc gaggtcctgg cggtagacga ccgatccacc gaccgcacgg
     1561 ctgagatcat cgccggcctc gcggcccttc ggcccggcag gctcaggatg ttgaaggtcg
     1621 accggctacc ggacggttgg ctcggcaaga accacgctct ctaccgcggg gccgcagagg
     1681 cctgcggcga gtggctgctc ttcaccgacg ccgacgtccg tttctctccg gactgcctgg
     1741 agctcgcgat gcgctacgcg gaacacaagc gcctggatca cctaacgctc gcgcccgagc
     1801 tcctctcgca cggggtggcc ctgaagagct tcgtgacggc ctttaccttc gtcttcgggg
     1861 ttacgcagcg gccgtggcgg gccgccgacc cgaccgcgag ggaggccgtc ggcgtggggg
     1921 ccttcaacct cctgaaaagg gaggcctacg ggcgggccgg cacccacgaa gccatccccc
     1981 ttcgtcccga cgacgacatg aggctcgcga agctgctcaa ggaggccggg tactcgcagg
     2041 acgtcgcata cgggacgggc tgcgtaagcg tggagtggca tcagaccctc ggcggcgcca
     2101 tcaggggcct cgaaaagagc atctttcccg gcgtggacta caggctctcg gccatcatcg
     2161 ccgcctcgct tctgctcttc ctgaccaacg tctggccctt cgccggggtc ttcctcgcgc
     2221 gccggcgggc catgaggctg ctctacggcg ccgacgtgct tgccatcgtc gccatgtacg
     2281 cccacggccc gaaggtctcc ggttactccc tctcgccgct gtacctcccc ctccacccct
     2341 tcgggatggc agccttcatc tacgccgccc tcagatccgc ctacgtggcg ctggtcaggg
     2401 gcggcatcca gtggcggggc acgttctatc cgctgcgcgt gttgaaggga gatgaccgta
     2461 gaggttagcg gtgggcttct cctgctgaaa gctgacagct aatagcccca gaggcgtatg
     2521 ctacagtcac tgctaagagc tagagagatg cgggaggagt gagctttgga taccagaaag
     2581 ggtgcgaagg accgctggga agaagcttac gccaggggct cggagcgcga ggccgagttc
     2641 tcgaccatgt cgggcgtgcc gatcaaacct ctctacacgc ccgatgacgt ggagggcgag
     2701 tacgacgaca agctcggcta tccgggcgag tacccgtaca cccgcggcgt ctacccgaac
     2761 atgtatcgcg ggaggctatg gacggtacgc cagttcgccg gttacggcac ggccgccgag
     2821 accaacgagc ggttcaggta cctcctggac cacggccaga acggcctctc cgtggccttc
     2881 gacatgccca ccctgatggg cctggatagc gattctcccc tcagcctcgg cgaggtcggg
     2941 accgagggcg tcgccgtgga ttccctggag gacatggagc ggctcttcga gggcatcccg
     3001 atgggcgagg tatccacctc catgaccata aacgccccgg cgatggtgct gctggccatg
     3061 tacgtcgtgg cggccgagaa gcagggtgtg gcggccgagg atctcgccgg cacaaaccag
     3121 aacgacatcc tcaaggagta catcgcccag aaggagtggc tcttcccgcc ggagccatcc
     3181 atgcgcgtct tccgcgacat gctcgtctac gccaccgagc acatgccgaa gtggaacacg
     3241 gtctccatct ccggctacca catccgcgag gcggggagca cggccgccca ggagctcgcc
     3301 ttcacgcttg ccgacggctt cgcctacgtc gaggctggca tagaagccgg cctcgacgtg
     3361 gacgacttcg ccccgagatt ctccttcttc ttcaactcgc acatagactt cttcgaggaa
     3421 atctgcaagt tccgcgccgc ccgccgcatc tgggccacgg tcctcaaaga gagatacgga
     3481 gcgagagacg agcggtccct gcggatgcgc ttccacaccc agaccgccgg tgtctccctt
     3541 acggcccagg agccctacaa caacgtggcc cgcaccgcct tcgaggcgct cgccgccgtg
     3601 ctcggcggca cccagagcct gcacaccaac tcgctcgacg aggcgctcgc gttgcccacg
     3661 gaagaagcgg tgcgcatcgc cgtcaggacc cagcagatag cggccctgga gaccggcgtg
     3721 gcgaacacca tagacccgct cggcggctcc tacttcgtgg aggccctgac cgacgagctc
     3781 gagcggcagg cgtaccacta cttcgagcag atagacgaga tgggtggcat gatccgggcc
     3841 atagagcagg gctttccgat gcgcgaggtc tccgaggcct cggcccgcta ccagcgcgaa
     3901 ctggaagaga acaggcgcca catggtaaac gtcaacatct acgaaccgca ggacgagcag
     3961 gacatggagc tacaccgcat cgaccccgag gtctcgaagg gccaggtaga gcgcctcgaa
     4021 agattgaagc agactcgcga ctacgacctc gtagaaaagc ggctcgtaga gctcagggaa
     4081 gcctgccagg gcgaagggaa caccatgtac ccgatcctgg acgccgtcag ggcctacgcc
     4141 acggtgggtg agatcagcgc caccatgcaa gaggtcttcg gcgattacag agagacccct
     4201 gtaatctaga gctatcagcc ttcagctgtc agctagacct gggtgtaacg tgggtctcta
     4261 acgggcggct aggcccagtt cctgatccgt ggcgtcgtgg ccgtacttgt ctcttggcgg
     4321 caagggccgg gacgcccagt cggggtcgaa gacgaacgac gacacggtgc ggtcgggggg
     4381 cagggcgccg ttgcccatgc cctttgcgta atcgtagtag agcttgacta tctgctccct
     4441 gctcacctgg ccggctgcgg ggtgcgcgag acacgcctcg cgccatttgc gaacgcgagc
     4501 gtacctgtcg tcttgcggca gatcgaagtc ctcgtaatac tccagaaacc agaaacgcat
     4561 aaagaacggc gtgtataccg tctcggccaa cccgaactcc tcgaagagga acgtgccgga
     4621 ggggttgtgc cgcacgagga attcgtccaa ccgggcgtac aggtcgagca tcccctgacg
     4681 gagctccgat ctcctcccgg gatcctggtt catcacgtag cggtagccct gggcggagaa
     4741 gcttccttcc atcgcgatca gcatgccctc gaccgcgtgc aggtagggat cccgcctggc
     4801 gacggcgggt tcgggaaaac ggtcttcgag gtactggagg atcaccatgc tttccttgat
     4861 aacctcgccc tcgccggtgt cgagcaccgg taacgccgtc gtgccgtgcg tcttctccag
     4921 cagccacccc gggcgcggac gcgtgatgtc tacgacgtgg aagcctacgt ggccgccaag
     4981 gccctttagc tcgaggagta tctccagccg ctgggagaaa ggacagaccg gtatgtggta
     5041 cacgaccggc acccgaagat tctgcccgat ggaggcatgg ctcacggctt ctctagtcaa
     5101 caggtctcct ccctcgtcgg atccggtgac ggagaatagc ataacgggcc gcccgtcttc
     5161 gcaaggtccg cgtggctctt gaggctcctg ccgtctgcta gaattcggga acgtgggagc
     5221 ggtttcggct ccgcacgtgg ggaccttgag cagaggagga ggtctatggc gatccgagag
     5281 accagcgtca ggcacgaggc gttgggactg gacgaggacg acctgcgccg gatgcactac
     5341 tacatgcttc tcgcccgcaa ggtggacgag cggtcctgga tcctcaaccg tcagggcaag
     5401 gcggcgttcg taatctcctg ccagggacag gagggttgcc aggtcggagc cgccttcaac
     5461 atgcgccccg gctacgatta cgtctacccc tactaccgcg accatgccat cgtgacgacc
     5521 cttggcgtga gcgcccgcga cgagttgctc gccctgctcg gccgcaagga ggacccgaac
     5581 agcggcgggc gtcagatgcc cggccacttc tccagccgcg agctcaacat cgtcaccgcc
     5641 tccgcgccca taggggtgca gtacccgcag gcggtgggaa cagccctcgc gttcaagatg
     5701 cgcggcgagg acggggtcgt gctcgcctgc ggcggcgagg cctcgaccag ccagggcgat
     5761 tggcacgagg ccatgaacct ggccggcata cacgatctgc ccgtcgtctt catgatcgag
     5821 aacaacgtct acgccatctc cgtcccctcc gagctacagg tggcaggctc catagccagg
     5881 agggcagaag gctacggctt ccccggccac gaggtggacg gcaacgacgt gctcgccgtc
     5941 tacgaggtgg cgaaggaggc gttcgagcgc gcccgcgccg gcgaggggcc aacgctcatc
     6001 gaagccaaga cctaccgaat gacggcccat tcctcggacg acgacgaccg caggtaccgc
     6061 gagcgcgagg agatcgagga gtggcgtcag aaggacccca tcgaccgctt cgaggggtac
     6121 ctgctcgagc acggcatcct cgacgaggcg gggcgggatg agatctccga gcgcatcagg
     6181 gccgaggtcg cggaggcgag ccagtacgcg gagaacgcgc caccggccgg ccccgaagac
     6241 gccctgaccg gcgtctacgc ggaggcctag ccatgacgac gatgaacctg cttcaggcca
     6301 tccacgaggg gctggccgag gagatgcgcg ccgacgagtc ggtgatggtg ctaggcgagg
     6361 atgtgggcag ggccggcggc gtcttccggg tcacgcaggg gttgcaggag gagttcggga
     6421 gcgcgcgggt catggacacg ccgctcgcgg agagcctcat cgtgggcgcc gccatcggcc
     6481 tctcggtcaa cgggatgcgc cccgtggccg agatacagtt cggcgacttc atcccgccgg
     6541 ccttcgacca gatcgtcaac gaggcggcca agttctacta ccgctccaac ggcgcctgga
     6601 acgtccccct gaccatccgc gccccctacg gcgtcgtgcc cggcggggcc ctctaccact
     6661 cgcagagcat agaggcctgg ttctgcaaca cgccaggcct caaggtcgtt gcaccgacct
     6721 cccccgccga cgcgaaagca atgctaaagg cctccatccg cgaccccaac cccgtcctct
     6781 tcctcgaaca caaacgcgcg taccgcctga taaaagaaga agtgcccgac gacggccccg
     6841 aggtccccct cggaaaagcc cgcgtccacc gcgaaggcac ggacatcacc gtcgtctcct
     6901 acggcctgat gctctacacg gtcctcgaag tggcggagag gatctcggag gagcacggcg
     6961 tggagaccga ggtcgtggag cccagaacgc tgtacccgat ggacacagag acaatcctcg
     7021 attcggcccg caagaccggc aaggttctgg tggtctccga ggccaacctc acgggctcgc
     7081 tctcgggtga gatcgccgcc atcgtggcgc gtgaggcctt cgagtggctc gacggccccg
     7141 tgatgcgcct cggcgccccc gacgtgccgg cggcaccctt cgccagatct atgctggacg
     7201 ggtttctgcc ctcggcggag gagatagaag gggccatgat ggagttggcc cgctactagg
     7261 atggacgtac cagggtacaa ccgcgaggcc tgggacaggc aggtcgagag cgggaacccg
     7321 tggaccgtcc ccgtcggccc cgaggtaata cgggcagccc gccgtgggga gtgggaggtc
     7381 ttgctcacgg agacgaagcc ggtcccgcgg gcctggtttc ccgagatggc tggcgccgac
     7441 gttctctgtc tcgcctcggg cgggggacag caggccccga tcttcgcggc cgccggggcc
     7501 agagttaccg tgcttgacaa ctcgcccggg caactggcgc aggacaggtt cgtagccgag
     7561 agggaggccc tggatctcga gaccctccag ggggatatga gggacctctc agccttcgcc
     7621 gacgagagct tcgacctcgt cttccacccc gtctccaacc tgttcgtgcc cgaggtcagg
     7681 cccgtctgga aagaggcgtt cagggtgctt cgaagcggtg gagccctgct cgccggtttc
     7741 ctgaaccccg tcgtctacgt cttcgacctc gagctcgccg atgatacggg cgaggtcagg
     7801 gtcaggtaca gccttccgta cgccgacgcc acgagcaaga gcgtagaaga tctccagcaa
     7861 cagatggagc ggggggagcc gctcgagttc agccacaccc tggaggagca gatcggcggc
     7921 caggtagagg ccgggttcct gatctccggc ctctacgaag accgccaccg cgacgacccg
     7981 atcgccgcct acatgcccac ctacgtggct accagggcga tcaaaccgta aaaacaggag
     8041 gagccgtggc caaaccggtg acgatgccgc aactgggcga gagcgtgact gagggcacga
     8101 taacccgctg gctgaaggcc gaaggtgagg tggtggagat cgacgagccc ctcgccgagg
     8161 tagatacgga caaggttaac gccgagctac cctcgcccgt ggccggcaag atcgagagcc
     8221 tcctcgtccc ggagggcacc acggtcgacg tgggaaccga gatcgtcctc gtagccgacg
     8281 acgatgcccc acgaaaggag agagtagccc tcgcggatga gttccctacc gcggagactg
     8341 tagcccgtcc cgcagcggaa gaactcgacc cggtggccct cgcaccacgc agcgagggca
     8401 acgggcgcgg cgaggtgaaa gacgccgcga cgctgcgccg gacgcgctcc tcaccggtgg
     8461 tccgacgcct cgccgcagag cacgacgtga agattgcgga tatccccggc tccggcacgg
     8521 gaggacgcgt aacccgaaag gatatcgaag gctatataga gcgaagcggc gccgcacgcg
     8581 gctcggtgac cgaagggcca atgctccagc gaatcgaagg agaggaacgc gcggccgtct
     8641 acgagggcga tggcatccag gacctcacga gcatccggcg ggccatagcg aaccgcatgg
     8701 ccaccagcaa acgtgaggcc ccgcacgcgt ggaccatggt cgaggtagat gtgagcgggc
     8761 tcgtcgcgct gcgtgagcgg gagaaggccg ctttcgtgga acgcgagggc gtgaagctca
     8821 cctacctgcc gttcatagtt agggccgtcg tcgagagcct ccgggagaac cccatgctca
     8881 actcggtgtg ggatggggac cgtatcatcc tcagaaagcg cataaacatc ggcgtcgccg
     8941 tcgacctgga agacgccctg atcgtccccg tcattcccga cgccgacgag ctgaacatca
     9001 ccggattggc ccgccgcatc gacgcgctcg taaagcgtgc ccgcgccggg gagctcggca
     9061 cgaacgacgt ctcgggcggt accttcaccg tcaacaaccc gggctccctg ggcagcgtgg
     9121 tctcgacgcc aatcatcaac cacccgcagg cggcgatcct ctccgccgaa gcgatagtca
     9181 agcgcccggt agtaatcggc gacgccatcg ccatccgaag catgatgaac ctcgaggtct
     9241 cctttgacca ccgcatcctc gacggtggag ccgccctccg gttcctgaac gccgtcaagg
     9301 gacgactgga ggcctacagg ccggagggcg gcctcggttg agcacgagtt cttcaaggag
     9361 gtagtgtcat ggttcaacag cagtcggcct ctgtcgaaac cctggaggcc atcaccgacg
     9421 cgttcaatgc ccacgatctg gatgccatca tggagttctt tgcggacgat tgttcgctgg
     9481 acatgccccg tgggccggag ccctggggac agcgcttcat gggcaaagag gccgtacgag
     9541 cggccctggc gacgcgtttc gagggcctac cagacgttca ctacggtgat ggacgacact
     9601 gggtctgtgg aaatatgggc gtgtccgagt ggacgctgac cggcacgatg gcagatggag
     9661 cccgggttcg agtacgtgga tgcgaccact gggagtttag cgacggtaag gtgatccgga
     9721 aggattcata ctggaagatc gtcgagaacc cggcatgaga taaggcaaaa ggcaacagat
     9781 ttttggtctt cctgaagcct gttgcctttt accttgacac acctcaagtt cacttttccc
     9841 taaacaaccg atccagctac cagcgcgagg gcgcgcacgg ggctgccggt gccgccggta
     9901 agcttgagtg gggatacgat gattagcgcg cctgtcggcg gcagggcgcc gaggttcgcc
     9961 agttgggtga cgccgtactt gttcgcgccc atgaggtagt agtgggctgg gaatgggggg
    10021 tcgaagcctc ccgcggcgcc ggcgtcgatg ccgacggtct cgaccccgac tccgacgagg
    10081 gggctctcct cggcgatcca gcgcacgcac tcggcgtcga agccgggcga gtgggggttc
    10141 ccgtcccggg cgttcaggaa cgcttccggg tccttggcgc gggcgtccca gcccgtgtgc
    10201 aaaagcagcc agcctccttc ggggagcggg ccatgatctt cttcgaaggc gcgtacatcg
    10261 tcgatggtga gcaggtagtc tgggtcttcg gcggtctcgg tggaacggtc gatcacgacc
    10321 gcaggggcga cgaggtggcg gggcggcacc tgcgagacgt cgttaccgtc tcggccgctg
    10381 atccagtgta cgggcgcgtc gaggtgggtg cccgtgtgct cgccgagctc gagccaggac
    10441 cacgcccacg cggggccccg ctcgtcgtag tggctgatct cgtgccgctt cagggccggc
    10501 gtctggg
//
LOCUS       DBNGVD010000404         6375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3194097, whole genome shotgun sequence.
ACCESSION   DBNGVD010000404 DBNGVD010000000
VERSION     DBNGVD010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3194097"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..494
                     /locus_tag="AC16CM_10905"
     CDS             309..494
                     /locus_tag="AC16CM_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3140384.1"
                     /translation="MGLLGDGGKDKDEGAMDRAKGRMKEAAGAMTGDKETKNKGRADQ
                     RRGKAKEKKGNLKDLLS"
     gene            872..1600
                     /locus_tag="AC16CM_10910"
     CDS             872..1600
                     /locus_tag="AC16CM_10910"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR3140385.1"
                     /translation="MRSKNLVLIMVVLAMAVVIAGCSSAGQSTGDQGQASQKKEAKKE
                     QPEKKEPAKKMTDEAKSKQVAPKKGTEQDAGSGENLVDPPEKKRLKLTVPAMKRINND
                     PIPTGLGTDEKLLHDYAGVHLSSTGFPWKKTSNVYIAGHRIGFPGTKSNLAFYDLEDL
                     KNGDKVYLEDADGRKYTYVVYEKLVVEPTNLSVLNPVKGKNIISLQTCTLPDYAKRVI
                     YRAELEGIEKPDDKAGDESQNSES"
     gene            1753..3198
                     /gene="xylB"
                     /locus_tag="AC16CM_10915"
     CDS             1753..3198
                     /gene="xylB"
                     /locus_tag="AC16CM_10915"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3140386.1"
                     /translation="MLLGLDLGTSSVKALLIGDDGEAVGEGSAPYEVRAPRPGWAESS
                     PGEWWEAVVEATGLAVGERGAEVTALGLSGQMHGVVLVGEGGSPLRPAVLWADARSGT
                     ELAAYGELDEELRRRLANPPAVGMAGPSLLWLRDNEPEAYAAAVWALQPKDWLRMRLT
                     GEVAAEPSDASATLLYDLSADDWSYAVVDHLGVREGLLAPLVSSADVAGELTAQAAEQ
                     LGLPRGLPVAAGAADTAAAMLGVGLSRTGPAQLTVGTGGQIVTPRESPEVDPSLRTHL
                     YRGALPGLWYSMAAIQNAGLALEWVRKVLGVSWEEVYEEAFAVPPGSGGVTFLPYLSG
                     ERTPRFDPGARGAWAGLGLDHTRGHLLRAALEGVAFALREGLEALEEPGGEDFEIRLA
                     GGGASGGSGDVWRQLLADVMGRPLWLLPDEISTVASARGAAFLAGLASGVYSSAEETP
                     TPDPRRSIHPGDADYGAAYERYKQLYPKLYG"
     gene            3331..4767
                     /locus_tag="AC16CM_10920"
     CDS             3331..4767
                     /locus_tag="AC16CM_10920"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIR3140387.1"
                     /translation="MAENLLSREYTDSLRENDAADARWLLAERVGRALSEAGPDGALG
                     TRERVMRLAVPAGRINPFRWLREQALFPKTYWAGRGERTGIAAVGAADIRVEGVSRGE
                     RSLSALLAALPDMEDSGARYYGGARFDALREPDSEWAPFAAYYFVLPRLELHVGDSET
                     TLICNLVLPRDVHNGPEIREQIEDLSLPGGALGASLPIPVRREDSPDFRGWERNIERA
                     LRAFSEGRLGKVVLARRAKIGFGGELDATLLLESLKEATPDCFHFYTEPEAGVAFLGA
                     SPERLFSREGNLVVSEAVAGTRPRGLSSEDDDGLREDLMHSAKDRSEHTYVRTGIEEA
                     LAPLCEELNLDGEVVEMKLARGRHLKSMVRGTLKEGVTDAGLLEALHPTPAVGGHPRD
                     GALAEIRDLEPFDRGLYAGPVGWFGAEASEFAVGIRSGLVRGRTLDLYSGAGIVAGSV
                     PDEEWAEIENKISDFTGMFGLEQGNAAS"
     gene            4790..>6375
                     /gene="menD"
                     /locus_tag="AC16CM_10925"
     CDS             4790..>6375
                     /gene="menD"
                     /locus_tag="AC16CM_10925"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIR3140388.1"
                     /translation="MEELVRCGVGLFCVAPGSRSTPLVAALAANEKARSLVHFDERGT
                     AFAALGYARATRRPAAWITTSGTAVANGLPAVVEASTDGVPMILLTADRPPELRQTGA
                     NQTIDQPDIFGGYVRWRFDLPAPDLSVDPAMVLTTVDQAAYRARRSPQGPVHLNLMFR
                     EPFLPEGEEEISGPSPWAEAEEPYTRYAATKPAVDETEVLALWRTLRRSKRGLIVAGR
                     LASREQGEAVSRLAGALGWPLLPDVGSQVRLGPESDNLVAYYDALLASETFAESHAPE
                     AVVHVGGRALSKRLEQFLGRSGPDPYVVVRENPFRLDPSHRVTHSIEADVLDFCAAMA
                     RVVEEDPPESDDTWMTGWRGASERVDHTLDGISSAAPNEPFVARSVSRNVPSDHGLVV
                     ASSMPVRDLDTYAAAGGAPVPVASNRGASGIDGTVATAAGFARGLGRPVTLLIGDLAL
                     LYDLNSLAMLRDVPVVVVVLNNDGGGIFSFLPVAERKEFFEPYFGTPQGVGFESAAEM
                     FGLHYERPETTGEFVEVYRGA"
BASE COUNT         1091 a         2051 c         2179 g         1054 t
ORIGIN      
        1 ccgagcagta gcccgcagag gatgacggcc aggacctgaa aggtaaaagg aacgggttgc
       61 agcgggatcg cgatctgggc cgccaccgcg gtgaccgccg ccatcaacgc cgcacgggtt
      121 atggtggatg tcctcaacga tgtcccttct gtggttccga agcgtggagt acgtccggcc
      181 gttgctccgc cctcctttta ccatcgtttg tctcgagttt cctgcgctag tagcccggga
      241 tgcaggcgtt cggccttctg cagccggtgg taagatgaag tcgtacatct ccagtagagg
      301 aggaacaaat gggtttgttg ggcgacggcg ggaaggacaa ggacgaaggc gccatggaca
      361 gggcgaaagg gcgtatgaaa gaggcggccg gagccatgac cggcgacaag gaaaccaaga
      421 acaagggacg cgcggaccag cgccgtggga aggccaagga gaagaaggga aacctgaagg
      481 atctgctgag ctagcgttcg cgtttcggtt ctgtagaagg cgtcagctgg acgtagcggc
      541 agacgccact tggcttcatt gagatattct agcgcccggc ggatttccgt cgggcgcttc
      601 ggctttcccg gtgtcggagg ggagaaccga tggccgtggc acgggcctcc gttacggggc
      661 gtctaacggg ctgaccttta cacccggggt cttgagccat aatatattca cctggcgaca
      721 gaggcgcgga cgggtggcat taccggggtg cgcgttgcag gtgacggaga ggcccgtaac
      781 ggagttttgg tccaaacgtt accttcttgc aatctttgat ctgtacgctt tccaggtacg
      841 gtagttcagg tggacggcga gaggtgatgt gatgcggtcg aaaaatctgg ttctgataat
      901 ggtggtcctg gcgatggccg tggtgatcgc tggctgttct tctgccggcc aatctacggg
      961 agaccagggt caggcctcac agaagaaaga ggccaagaag gagcaacccg agaagaagga
     1021 gcccgccaag aagatgacgg acgaggcaaa gagcaagcag gtcgcgccga agaagggaac
     1081 ggagcaagac gctggcagcg gggagaacct ggtcgatccc cccgagaaga agcgcctcaa
     1141 gctcaccgtt ccggccatga agcggataaa caacgatccc atcccgaccg gcctcggcac
     1201 cgacgagaag ttgctccacg actacgccgg cgtgcacctc agctccacgg gcttcccgtg
     1261 gaagaagacg tccaacgtgt acatcgccgg gcaccgcatc gggttccccg gcacgaagag
     1321 caacctggcc ttctacgacc tcgaggacct gaagaacggc gacaaggtct acctggaaga
     1381 tgccgacggc aggaagtaca cctacgttgt atacgagaag ctcgtcgtgg agccgactaa
     1441 cctcagcgtg ttgaaccccg tcaagggcaa gaacatcatt agcctgcaga cctgcacgct
     1501 gccggactac gccaagcgcg ttatctacag ggccgagctc gaaggcatcg agaagccgga
     1561 cgacaaggcc ggggacgagt cacagaactc cgaatcctag gccgcgaacg acctcacgag
     1621 aggggagggg aacctccagg ttcccctccc ctctccgccg gaactagagt caagtcaatg
     1681 acaggcgtct accgtggaag agagaggtgt ctcttgataa cggcgaaccc ggaatcggtc
     1741 tagggcactc tcgtgctgct cggcctcgac ctgggaacga gctccgtcaa ggcgttgctc
     1801 attggggacg acggtgaggc cgtcggcgag ggctcggccc cttacgaggt gcgggccccg
     1861 cgaccggggt gggccgagtc ctcacccggg gagtggtggg aggccgtcgt ggaggcgacc
     1921 gggttggccg tcggagagcg cggtgcggag gttacggctc tcgggctctc gggccagatg
     1981 cacggcgtcg ttctcgttgg cgaggggggc tcgccgttac ggcccgccgt gctgtgggcc
     2041 gatgccagat ccgggaccga gctcgcggcc tacggagagc tcgacgagga actgcgccgc
     2101 cggctcgcca atccgcctgc cgtgggcatg gccggaccca gcctcctgtg gctgcgggac
     2161 aacgaacccg aggcctacgc ggccgcggtg tgggccttgc agcccaagga ctggctgcgc
     2221 atgcgcctta cgggcgaggt cgcggcggag ccatctgacg cgtcggccac cctgctctac
     2281 gacctctccg cggacgattg gtcctacgcg gtagtggatc acctcggggt tcgagaggga
     2341 ttgctggccc cccttgtctc ctccgccgac gtggccggag agctcacggc gcaggccgct
     2401 gagcaactcg ggttgccgcg tggactgccg gtcgcggcgg gggctgccga cacggccgcc
     2461 gccatgctcg gcgtggggct ttcgcggact ggacccgcgc agctaactgt cggaacgggg
     2521 ggccagatcg taacgccccg cgagagcccg gaggtggatc ctagcctccg tacccatctg
     2581 taccgtggtg cactgccagg gctctggtac tcgatggccg ctatccagaa cgccggcctc
     2641 gccctggagt gggtccgcaa ggtgctcggc gtctcctggg aggaggtcta cgaagaggcc
     2701 ttcgccgtgc cgccgggttc cggcggcgtc accttcctgc cctatcttag cggtgaacgt
     2761 acgccgcgct tcgaccccgg tgcccgcggc gcctgggccg gcctcggcct cgaccacacc
     2821 cgcggccacc tccttcgcgc cgccctcgaa ggcgtcgcct tcgcgctgcg agaaggcctc
     2881 gaggcgctgg aggagccagg gggggaagac ttcgagattc gcctcgccgg aggcggtgcg
     2941 agcggggggt cgggagatgt ctggcgtcag ctcctggccg acgtgatggg ccgcccgctg
     3001 tggcttctgc cggacgagat ctccaccgtc gcgtcggccc gcggcgcggc ctttctggcc
     3061 ggcctcgcct ccggcgtcta ctcttcagcc gaggagacgc caaccccgga ccccaggaga
     3121 tccatacatc ccggggacgc ggactacggg gcggcctacg aacgctacaa acaactctat
     3181 cctaagctct acggttgagc ttccgaacct ggcctgcggc caatttattg cgttctgggg
     3241 ccgaaagcag accgatgacg gacgcaaatt gtgagcgaac cctctatacg attagctact
     3301 ccttgctaca ctaccgcatg agatggtgag gtggctgaga acttgttgag cagggagtat
     3361 acggattcct tgagggagaa cgacgcggct gatgcccgct ggctactggc cgaacgcgtg
     3421 ggccgcgctc tctcggaagc aggtcccgac ggagctctgg gcacgcgcga gcgtgtcatg
     3481 cgtctggccg tgcccgccgg gcgcatcaac cctttcaggt ggcttcgtga acaggcgctg
     3541 ttcccgaaga cgtactgggc tggccgcggg gagagaaccg gaatcgccgc ggtcggagcc
     3601 gccgacatcc gggtcgaggg cgtctccagg ggcgaaaggt ccctatccgc gctgctggcg
     3661 gcgctgcctg atatggagga ctcgggggcg cgttactacg ggggcgcgcg tttcgatgct
     3721 ctgcgtgagc ccgactctga gtgggccccg ttcgccgcct actacttcgt cttgccccgc
     3781 ctcgaactgc acgtgggcga ctcggagacc accctcatct gcaacctggt acttcccagg
     3841 gacgtgcaca acggccccga gatacgggag cagatagaag acctatcgtt gcccgggggc
     3901 gcgttgggcg cctcgttgcc catccccgtc cggcgcgaag atagcccgga cttccggggg
     3961 tgggagcgga acatcgaacg ggcgctccgg gccttctcag aggggcgtct gggcaaggtc
     4021 gtgctcgccc gcagggcgaa gattggcttc ggcggggagc tggatgccac gttgctcctc
     4081 gaaagcttga aggaggccac gcccgactgc ttccatttct ataccgagcc ggaggccggg
     4141 gtggcgttcc tcggggcctc tcccgagcgc ctgttcagcc gcgagggaaa cctggtcgtg
     4201 agcgaggccg tggccgggac gaggccgcga ggtctatcgt cggaggacga cgatgggttg
     4261 cgtgaggacc tgatgcacag cgcgaaggac cgctccgagc acacctacgt gcgcaccggc
     4321 atagaggagg cgctggcgcc gctctgcgag gaactgaacc tcgacggtga ggtggtcgag
     4381 atgaagctgg cgcggggcag gcatctgaaa tccatggtgc gcggcaccct caaggagggc
     4441 gtaaccgacg ccggcctgct cgaggccctg cacccgacgc ccgccgtcgg cggccacccg
     4501 cgcgacgggg cgctcgcaga gataagagat ctggaaccct tcgaccgggg cctctacgct
     4561 ggtcccgtcg ggtggttcgg ggccgaggct tcggagttcg ccgtcggtat caggtctggg
     4621 ctcgtccggg ggcggaccct ggatctctac tcgggggctg ggatcgtcgc cggttccgtg
     4681 cccgatgagg agtgggccga gatcgagaac aagatctccg acttcaccgg gatgttcggg
     4741 cttgaacagg gaaacgccgc gagctaaccg cctctgggcg agccttatcg tggaagaact
     4801 cgtccgctgt ggcgtcggcc tcttctgcgt ggcacctggc tcccgctcca caccgctcgt
     4861 cgccgccctc gccgccaacg agaaggcacg tagcctcgtc cacttcgacg agcgtggaac
     4921 cgccttcgcg gccctgggct acgcgcgcgc cacccggcgt cctgcggcct ggatcaccac
     4981 ctctggcacc gcggtcgcca acggcctgcc cgctgtggtc gaggcctcga cagacggcgt
     5041 gcccatgatc ctgctcaccg ccgaccgtcc ccccgagcta cgccagaccg gggccaacca
     5101 gaccatcgac cagcccgaca tcttcggcgg ctacgttcgc tggcgcttcg acctcccggc
     5161 ccccgacctc tccgtcgacc cggccatggt acttaccacc gttgatcagg ccgcctaccg
     5221 cgcccgccgc agcccccagg gccccgtcca cctcaacctg atgtttcgcg aaccgtttct
     5281 gccagaaggt gaagaggaga tctccggacc atctccctgg gcggaggccg aggaacccta
     5341 cacccgctat gccgcgacga aaccggccgt ggacgaaacg gaggttctgg ccctctggcg
     5401 gacgcttcgc cggtcgaaac ggggcctcat cgtggccggc cggctagcct cccgagagca
     5461 gggcgaggcc gtgtcgcggc tcgccggcgc cctgggttgg ccgctcttgc cagacgtcgg
     5521 ttcccaggtg cgcctcggcc cggaatcgga taacctcgta gcctactacg acgcgctgct
     5581 ggccagcgag acgttcgcgg agtcccacgc gcccgaggcc gtggttcatg tcggggggag
     5641 ggccctctcc aagcgcctgg agcagttcct gggccgcagc gggccggacc cctacgtcgt
     5701 cgtgcgcgag aacccgttca ggctcgaccc ctcccaccgg gtcacccaca gcatcgaagc
     5761 tgacgtcctg gacttctgcg ccgccatggc aagggttgtc gaggaagacc cgccggaaag
     5821 tgatgacacc tggatgacgg gttggcgggg ggcttcggag agggtggatc acaccctgga
     5881 cggaatctcc tccgcggctc cgaacgagcc gttcgtggcc cgcagcgtct cgcgtaatgt
     5941 gccctcggac cacgggctcg tcgtcgccag cagcatgcct gtgcgcgacc tggacacata
     6001 cgcggcggcc ggcggcgcgc ccgtgcccgt cgcctccaac aggggcgcga gcggcatcga
     6061 cggcaccgtc gccaccgcgg cgggcttcgc gcggggtctc ggaaggcccg ttaccctcct
     6121 cataggggat ctggcgttgc tgtacgacct caactccctc gccatgctgc gcgacgtgcc
     6181 cgtcgtcgtg gtggtgctca acaacgatgg tggggggatc ttctccttcc tccccgtcgc
     6241 cgaacgcaag gagttcttcg agccctactt tggcacaccg cagggcgtgg gcttcgaatc
     6301 cgccgccgaa atgttcgggc tgcactacga gcgtccggag acgaccggag agttcgtcga
     6361 ggtctaccgc ggggc
//
LOCUS       DBNGVD010000405         3197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5015953, whole genome shotgun sequence.
ACCESSION   DBNGVD010000405 DBNGVD010000000
VERSION     DBNGVD010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5015953"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..921
                     /locus_tag="AC16CM_10930"
     CDS             367..921
                     /locus_tag="AC16CM_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140389.1"
                     /translation="MRSPLGLLGSLVLLLALPIAVLTQVLVGSGSAVAVHLALAAGCV
                     LVSFSAFGFGTPRWLAWIGCLSTGAFALIFLLQAVSTLIGNESFSYFTDEVLGFWPEK
                     VLLSLIAFWLVGMLLTSSRGKSRVLGFLAMAIVVCVEAYVYFGILVLGTNPFVETAVV
                     KLAYLLPFVWLLFESAKRRPGTGP"
     gene            1070..2023
                     /locus_tag="AC16CM_10935"
     CDS             1070..2023
                     /locus_tag="AC16CM_10935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3140390.1"
                     /translation="MNETFLEAGRRGKNTWWRYLLGVVFIVFMWFVLGGRASLLLGNA
                     LGISPELIAADASSAGPIAGYLVISASFPVFLLGTVLAVTFLHRRSPLTLVTDRRRVD
                     WRRVGAGLGVWLTLVVLASCVGFLLDPSAFSLGSNLAAFVPFALLALVLTPVQATAEE
                     LFFRGYLVQGASLISSNFLFLAVTSGALFMLPHLANPGLDSGFLLVALYHFGFGAFLA
                     WVSLRDGTLELAIGAHTANNLFGAIVLGFEGSALNTPSLFLTDRFVPAYSLIQFVVTA
                     AIFYVVVFVFFKSRTKTAPKEVRFSRGGSHLPGRTFGDKSR"
     gene            2033..2449
                     /locus_tag="AC16CM_10940"
     CDS             2033..2449
                     /locus_tag="AC16CM_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3140391.1"
                     /translation="MTRRNPEGRLDFETLRQAIERSDPDLMLGFYAEDARLSIVNVHT
                     PHASPFELCGKGEIAKHLRVAFGQEASHRVGRDATVGEDRVTFREACKYPDGDRVWVE
                     TTLEVHDGKIVRQVDVVASDAGTTRQEGVGRGPPPS"
     gene            2530..>3197
                     /locus_tag="AC16CM_10945"
     CDS             2530..>3197
                     /locus_tag="AC16CM_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3140392.1"
                     /translation="MSAVASVVRRHPIITFFVLSYAISWAFLPIDAVGFLPSGPLFAA
                     LIVIPVTQGWAGLKGLGSRMVRWRVRWYWYLVAIGVPLAVHLLYVGLNVARGAGAPSL
                     AFASLTTFLMLFAIRLINPADGPLGEHPGWNGVALPGLQATYSPLVSTAILAVLVAGW
                     HLPLFFLEEGGLQPPILVGGLATTMAVTFWYAWLFNHTRGSILLVVAAHSVEGSIQAA
                     GWIY"
BASE COUNT          490 a          990 c         1039 g          678 t
ORIGIN      
        1 agggcgtcat ttacatcggc gatcaccttg gcttcgcata ttcggcaccg cagcgttcgt
       61 ctggactaat cggctagttg ggggctgggc gctcaggcgt tccggaaaca cgcgagatcg
      121 ttgaccagga ggctaccggg tactactagc gcccatgacc cgagataccc gtagacgata
      181 cccgtagttc tactggttca ttgtcgctcg tcgcgccgta gagttcgctt tgtcgggcaa
      241 ggcgtcccgc aagagcggga gaaaggggta cgcgacatga cggattatct ccggagcgtg
      301 gacccaggag gagccggaac ctcgccgagc gtcgtggcgc aacaagaggc gtcaagaggt
      361 tcgggcatgc ggtcaccact agggttgcta gggtcactcg tcctcttgct ggcgctcccc
      421 atcgcggtgt tgacccaggt acttgtcggc agcggctcgg cggttgccgt ccatctcgcg
      481 ctcgctgcgg gttgtgtgct cgtttctttt tccgcctttg gtttcggaac gcctaggtgg
      541 ctggcgtgga tcgggtgcct ctctaccggc gccttcgcgc tcatcttctt gcttcaagcc
      601 gtgagcacgc tgataggaaa tgaatcgttc tcctacttca ccgacgaggt gctcgggttc
      661 tggccggaga aggtattgct cagcctcatc gcgttctggc tcgttgggat gttgctgacc
      721 tccagccgag gcaagagcag ggtcctgggt ttcctcgcta tggcgatagt cgtgtgtgtg
      781 gaagcgtacg tgtatttcgg cattctcgtc cttggcacca acccgtttgt ggagacggcc
      841 gtcgtgaagt tggcgtacct cctgccgttc gtctggctcc ttttcgagag cgcgaagagg
      901 cgccccggga caggaccgta ggacctccgc tccccgcgcc gcggggtcct aaggtccgcc
      961 gaaggcgtgc cttgagtgct ggaatagttg gctcttccaa tagcagggat gcctccgctg
     1021 gcggagccgc cgacatcgga tgagacgaac agcagaaagg agtggcggcg tgaacgaaac
     1081 cttcctggag gccggaaggc ggggcaagaa cacctggtgg cgctacctgc tgggcgtcgt
     1141 cttcatcgtg ttcatgtggt tcgtactggg gggtagggca agcctcttac tcggcaacgc
     1201 gctcggtatc tccccggagc tgatcgccgc ggacgcttcc tcggcagggc cgatcgccgg
     1261 ctacctggtc atcagcgcct catttccggt gttcctgctc gggaccgtgc tcgccgtaac
     1321 gtttcttcac cggcggagcc cccttacgct cgtgaccgac cggcgacgcg tcgactggag
     1381 acgggtagga gcgggcctcg gcgtgtggtt gacgctcgtc gtcctggcga gctgcgtcgg
     1441 gtttcttctg gatccctccg ctttctcatt gggatcgaac ctggcggcct tcgtgccctt
     1501 cgccctcctg gccctcgtcc tcaccccggt ccaggctact gccgaggagt tattcttccg
     1561 cggctacctc gtgcaggggg cgagtctcat ctcctccaac ttcctctttc tcgcggtcac
     1621 ctcgggcgcg ctgttcatgc tgccgcacct cgccaacccg gggctggact cgggcttctt
     1681 gctcgtggcc ctctaccact tcgggttcgg cgccttcctc gcctgggtct ccttgaggga
     1741 cggcacgctg gagctcgcca tcggcgcgca taccgccaac aacctgttcg gggccatagt
     1801 gcttggcttc gagggttcgg ccctgaacac cccgtcgctc ttcctcaccg atcggttcgt
     1861 ccccgcgtac agcctgatcc agtttgtggt gacagcggcg atcttctacg tggtggtctt
     1921 cgtgttcttc aagagtcgta ccaagacggc tccgaaggag gtccgcttct cgcgcggtgg
     1981 gtctcatctg cctggaagga ctttcggaga caagagtagg taggtgcagg atatgacccg
     2041 gcgaaatccc gaagggagat tggacttcga gactttgcgc caagccatcg agcgaagcga
     2101 cccggacctt atgctcggct tctacgccga ggacgcccgg ctgagcatcg taaacgtcca
     2161 caccccgcac gcttcccctt tcgagctgtg cggcaaggga gagatagcca agcaccttcg
     2221 cgtggccttc ggccaggagg cgtctcatcg cgttgggcgg gatgccaccg tcggtgagga
     2281 tcgggtgact ttccgggagg cgtgcaagta cccggacggc gaccgggtgt gggtcgaaac
     2341 gacgctcgaa gtgcacgacg gaaagatcgt ccgccaggtg gatgtggtgg caagcgacgc
     2401 aggaacaacg agacaagagg gggtcggccg gggaccgcca cccagctgag gtacgtgcgc
     2461 cgggggaggc gatcgtgccg gacccgcttc ggtgcccatg caagccatcg agaaggagga
     2521 tcgaccgcaa tgtccgccgt agcaagcgtg gtgagacgac accccataat cacgttcttc
     2581 gtcctgagtt acgcgatctc ttgggccttc ttgccgatcg atgccgtggg gttcttgccg
     2641 tccggaccgc tcttcgccgc cctcatcgtg atccccgtaa cccagggctg ggcgggcctg
     2701 aaagggctcg ggagccggat ggtccgctgg cgagtgagat ggtactggta cttagtggcc
     2761 ataggagtgc cgctcgcggt ccacctgctc tacgtggggc tcaacgtcgc gagaggggcg
     2821 ggcgctccgt cgctggcctt cgcctccttg accaccttcc tcatgctgtt cgcgatccgc
     2881 ctgatcaacc ccgcggacgg gccgcttggc gagcatccgg gctggaacgg tgtcgccctc
     2941 ccgggactgc aggcgaccta ctcgccgttg gtgtccaccg cgatcctggc cgtgctggta
     3001 gccggctggc acttacccct cttcttcctg gaggagggcg ggttgcagcc accgatactc
     3061 gtaggcggcc tggcgacgac gatggcggtg acgttctggt acgcgtggct tttcaaccac
     3121 acccgcggca gcattctcct ggtcgtcgcg gctcactccg tcgaggggag catccaggca
     3181 gcgggttgga tctacat
//
LOCUS       DBNGVD010000406         3667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3740525, whole genome shotgun sequence.
ACCESSION   DBNGVD010000406 DBNGVD010000000
VERSION     DBNGVD010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3740525"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..866)
                     /locus_tag="AC16CM_10950"
     CDS             complement(171..866)
                     /locus_tag="AC16CM_10950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3140393.1"
                     /translation="MAGPFDGYEWIDLSHTLEEDIPAYPTHARFGRTLYESYEYGDPA
                     RHHGLTISEHTGTHMDAPLHFVPEGPAHYGTDEIPLDRLAGRAATIEATDLGAGDLLK
                     AARIEAWEEEHGPIEAGDRVLIRYGWDRRWATGPGGRRFLESWPGLSSGAAEYLVEKG
                     VSLVGCDTLAVDATVSTENPAHHALLGNEVYIVENLNNLDRLPPFCLFIALPLKIRGG
                     SGSPVRAVALVPR"
     gene            complement(866..2200)
                     /locus_tag="AC16CM_10955"
     CDS             complement(866..2200)
                     /locus_tag="AC16CM_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140394.1"
                     /translation="MAGNVSPESAPRNRYIPRGHPVSWWMLTVTTGAILICSIDRALL
                     PTVLPGILEEFDLSETAGGVLVGLSFAGTMAGGLILGVFGDSLGNGVRRAYTWAVAVA
                     IVVVSAIATAVSQTLLQLQVLRFVMGFGTGGMEPVNVAMVGEWWQKEDRGFAVGTHHT
                     GFPIGQFVGPLLIGAVLLIGTWRTVFLFLPLLAIPIVVLQIVLARRENLRKVNAWIEE
                     HDMTPSVTERELDEERDEQPVATAWQAMKLALAERNVRLSVVANFLFLWAETGVVTFL
                     TLQLTEEAGLSLAAASVIAGASGITGWMGQIFWGTVSDHRGRKWSLGILAVGWAVTVL
                     LMIFISSGALGWIILIGWGVFRNSPFPVMYASIIDAVPDGASSGLGLMIGIGLGSAGF
                     LAAPVAGFIVQHYGFTWHYVFLAAICLLTLVPISMIRETSAGQDEPAVPQGT"
     gene            2399..3268
                     /locus_tag="AC16CM_10960"
     CDS             2399..3268
                     /locus_tag="AC16CM_10960"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3140395.1"
                     /translation="MIFARNLGEPEGPVALGDGSWLVVEMAPERGCVTRLDGEGRSEG
                     VIARTGRPNGLAVDREGAIWVAESRDPSLIRLDLQGRAEVILTACDGEPFLFPNDLCF
                     GPDGALYMTDSGLLFEDFAPGGEIREDFMEAEIDGRIYRIDPGTAEIEKIDSGLRFAN
                     GIAFGPDENLYANDTFTGMVYRYRYEDGKIAGDREDFANVVRGEPTDRVVGPDGMAFG
                     TDGNLYVAVFGQGDVTVLSPSGGVVERIETRGSLPTNVAFGLDEQKIYVTEDEFGHVE
                     AFEVETGGLGLYT"
     gene            3282..>3667
                     /locus_tag="AC16CM_10965"
     CDS             3282..>3667
                     /locus_tag="AC16CM_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIR3140396.1"
                     /translation="MKEAFRVGLTRDFLREDGTVGWGDIGLASLDEADGIEWEFLPEG
                     GLELTAEQVGSYDAMILLSPRVSSATVEGADRLALVARFGVGYDNVDLGACTRNGVAV
                     AITPDGVRRPVATSILALLLALSHRLL"
BASE COUNT          657 a         1188 c         1230 g          592 t
ORIGIN      
        1 caccgaaggg gctgtccggt aaggcagact ccggtcagag aactcagatg ccgggcactt
       61 ctcgtttatc gagcgccccg acgtgggcgc caacgaggtg ctccaacctc tcggcgtcgg
      121 tagccgcctg gagaggtcac ccggaagacc gagaggttgc ccggcggcta tcagcgcggc
      181 acgagcgcga cggccctcac gggcgagccg gaaccacccc ttatctttag cggtaacgcg
      241 atgaagaggc agaacggcgg cagccggtcc aggttgttca ggttctcgac gatgtagacc
      301 tcgttgccga gcagtgcgtg gtgggccggg ttctccgtgg agaccgtggc gtctacggcc
      361 agcgtatcgc acccgactag cgagacaccc ttctctacca ggtattcggc ggccccgctg
      421 gagaggcctg gccagctttc gaggaagcgt cgtccccccg ggccggtcgc ccacctccgg
      481 tcccatccgt accggatcag gaccctgtcc cccgcctcga tcggaccatg ctcttcctcc
      541 caggcctcta ttcgtgcggc tttcagaagg tcgccggccc cgaggtcggt ggcctcgatg
      601 gtcgctgcgc gcccggcaag gcggtcaagt gggatctcat cggtgccgta gtgggccggt
      661 ccttcgggga caaagtggag gggggcgtcc atatgggttc cggtgtgctc gctaatggtg
      721 aggccgtggt ggcgggcggg gtcaccgtac tcgtaggact cgtagagggt tctgccgaag
      781 cgggcgtggg tggggtaggc tgggatgtct tcctccagcg tgtgggagag gtcgatccac
      841 tcgtaaccgt cgaacggccc agccactagg tcccctgcgg cacggccggc tcatcctggc
      901 ccgccgaggt ctcccggatc atcgagatgg gcaccagcgt caacaggcag atggcggcga
      961 ggaacacgta gtgccaggtg aagccgtagt gctggacgat gaacccggcc actggggcag
     1021 cgaggaagcc agccgagccc aggcctatgc ctatcatcag gcccagaccc gaggacgcgc
     1081 cgtcggggac ggcatctatt atggaggcgt acatgacggg aaagggcgag ttgcggaaga
     1141 cgccccagcc gatgaggatg atccaaccca gggcgccgct gctgataaag atcatgagca
     1201 gaacggtcac ggcccagccg acggccagta tgccaagcga ccacttccgc cccctgtgat
     1261 cggagacggt gccccagaag atctggccca tccaccccgt tatcccggag gcccccgcga
     1321 tcacggaggc ggcggccagt gagaggccgg cctcttcggt gagctgcagc gtcaagaaag
     1381 tgaccacccc ggtctcggcc cacaagaaca ggaagttggc gacgaccgag agcctcacgt
     1441 tacgctcggc gagcgccagc ttcatggcct gccaggcggt cgccacgggt tgctcatcgc
     1501 gttcttcgtc gagctcgcgc tccgtcaccg atggggtcat gtcgtgttcc tcgatccagg
     1561 cgttcacctt cctcaggttc tcccgccggg cgagcactat ctgcaagacg acgatgggta
     1621 tggccagcag cgggaggaac agaaacaccg ttcgccaggt accgatcagg agaacggccc
     1681 cgataaggag gggacccacg aactgcccta tggggaagcc cgtgtggtgc gtaccgacgg
     1741 cgaagcccct gtcctccttc tgccaccact cgccgaccat ggccacgttg acgggctcca
     1801 tcccgcccgt cccgaagccc atgacgaacc gcaagacctg taactgcaga agcgtctgcg
     1861 agacggccgt ggcgatggcc gagacaacca ctatggccac ggccacggcc cacgtgtacg
     1921 cccgcctaac gccattgccc agagagtcgc cgaacacgcc gagaatgagg ccgccggcca
     1981 tggtgccagc gaagctcagc cccaccagta caccgccagc cgtctcgctc aggtcgaact
     2041 cctcgaggat gccgggcagg accgtcggaa gcagggcgcg atctatagag cagatgagta
     2101 tggcaccggt ggtcaccgtg agcatccacc acgaaaccgg gtggcccctc gggatgtacc
     2161 tgttgcgggg cgccgattcc gggctgacgt ttcctgccat gcctctcctc ttctgggctc
     2221 cacgcgacca tagcggagcc ccacagcaac gctgccgcaa ccggtccttc cctcgacccg
     2281 ggcactatac cccggcccgg ctgtccgcgg caaccatgcg aaggtaggac ccggaatccc
     2341 acgccggaac ccacggatcc tgtatattga acgggaccgc ggcgggaggt ggaggcgcat
     2401 gatcttcgcg aggaatctgg gcgagcccga gggtccggtc gcgctgggtg atggaagctg
     2461 gctggtcgtc gagatggcac cggagcgagg ctgtgtgacc cggctcgacg gagaaggacg
     2521 atcggagggc gtgatcgccc gtaccggacg tccgaacggg ctggccgtgg accgagaggg
     2581 cgccatctgg gtggccgagt cccgagaccc ttccctgatc cggctcgacc tccaggggag
     2641 ggccgaggtg atcctgaccg cgtgcgatgg cgagccgttc ctctttccaa acgacctctg
     2701 tttcggaccc gacggcgccc tgtacatgac cgactcgggg ctgctcttcg aggacttcgc
     2761 gccagggggc gagatacgcg aggacttcat ggaagcggag atcgacggcc gaatctaccg
     2821 catagatcct ggcacggcag agatcgagaa gatagattcc ggcctccgct tcgccaacgg
     2881 catcgccttc ggcccggacg aaaacctcta cgccaacgac accttcaccg gcatggtgta
     2941 ccgctaccgc tacgaggacg gaaagatagc gggcgacagg gaagacttcg ccaacgtggt
     3001 tcgcggggag cccacggatc gagtcgtcgg tcccgacggc atggctttcg ggaccgacgg
     3061 caacctctac gtggcggtct tcggccaggg cgatgtgacg gtgctatcgc cttctggggg
     3121 cgtggtggag cgcatagaga cgcggggaag cctgccgacc aacgtggcct tcggcctgga
     3181 tgaacagaag atctacgtca cggaggacga gttcggtcac gtggaggcct tcgaggtcga
     3241 gaccggggga ttggggctgt acacctgagg gggtgtcgcg cgtgaaagaa gctttccgcg
     3301 tcgggctgac ccgcgatttt ctccgcgagg acggcacggt aggctggggc gacatcggcc
     3361 tcgcgtccct cgacgaggcc gacgggatcg agtgggagtt cctgccagaa ggcgggctgg
     3421 agttgaccgc agagcaggtc ggctcttacg acgccatgat cctgctgtcc ccccgcgtaa
     3481 gctccgccac cgtggaaggc gctgatcgcc tggccctcgt cgcgcgcttc ggcgtcggct
     3541 acgacaacgt cgatctggga gcctgcaccc gcaacggcgt ggccgtcgcc atcacccctg
     3601 atggcgtgcg ccgtcccgtg gcgacctcta tcctggccct gctcctggcc ctgagccaca
     3661 ggctgct
//
LOCUS       DBNGVD010000407         4818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2101013, whole genome shotgun sequence.
ACCESSION   DBNGVD010000407 DBNGVD010000000
VERSION     DBNGVD010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2101013"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..961
                     /locus_tag="AC16CM_10970"
     CDS             14..961
                     /locus_tag="AC16CM_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIR3140397.1"
                     /translation="MAQTMGIHHVTAISGEPQRNVDFYAGVLGLRLVKKTVNFDDPET
                     YHLYYGDGGGNPGTIMTFFPWAHASGGRIGAGQLIVTSFSVPISSLGYWTERLVEGGV
                     RFERPRDRFGDTVLTFEDPDRLRIELVATEDARPGWTGGTVPEEHSVRGFHHVALAVE
                     DIESTAGLMTGVLGFGQVDETEGRIRLSAGEGGPGNTVDVIGATGFPRGSMGVGTVHH
                     VAFRVPDEETQLALREEVSGLGYNVTPVLDRNYFRSIYFREPGGVLFEIATDPPGFAV
                     DEDEAELGTHLKLPPWLETRRERLEEVLPPLVVPEGESA"
     gene            958..1596
                     /locus_tag="AC16CM_10975"
     CDS             958..1596
                     /locus_tag="AC16CM_10975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140398.1"
                     /translation="MTPDLGFDHRYLPAGDRGGATLLLLHGTGGNEEDLIPLGQELLP
                     GAAILSPRGKVSEHGAPRFFRRLAEGVFDHEDLLFRTHELAAFVEAAAEEYGFDRSKI
                     IAVGYSNGANVAASTILLHPGLLRAAVLFRAMVPFEPESAPDLTGMPVFLAAGRMDRM
                     IPPDNTQRLAEILTGAGADVDLRWRNVGHPLTYEEVGEAGEWIARVLPGLDT"
     gene            1819..3480
                     /locus_tag="AC16CM_10980"
     CDS             1819..3480
                     /locus_tag="AC16CM_10980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3140399.1"
                     /translation="MAKPVILAVDDDPQVLRAVERDLRRRYAREYRVLRADSGESALD
                     TLGRLKLRGDPVALFLVDQRMPRMTGVQFLEEAIERYPEAKRALLTAYSDTEAAIRAI
                     NEVGLDYYLQKPWDPPDQNLYPNLDDMLGDWRADYRPPFEGIRVVGDRWSPGSHRTRD
                     FLARNRVPYRWLDVEGSKEAEEILEITDHGTPALPLVVYEDGTYAEAPENREIAEKIG
                     LQTRAEQEFYDLLIIGGGPSGLAAAVYGASEGLKTVLVEREAPGGQAGTSSRIENYLG
                     FPKGLSGGDLARRAVDQARRFEVEILTPQEVASVRVEDPYRIVTLSDGTEISCFALII
                     TTGVSYRKLNAPGIERLTGRGVYYGAAQTEALSCRDEDVYIVGGANSAGQAAMFFSDK
                     ARTVSLVCRGDDLRAGMSDYLVRQIEATDNIEVLLGTTVASADGEDHLENLTLKHAET
                     GKSETVTAHSLFIFIGAAPMTGWLDGLVERDERGFILSGPDLLRDGKRPEGWRPQRDP
                     FLLETSVPGIFVAGDVRHGSIKRCASAVGEGSIAVQFVHRYLNEV"
     gene            3483..>4818
                     /locus_tag="AC16CM_10985"
     CDS             3483..>4818
                     /locus_tag="AC16CM_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140400.1"
                     /translation="MSGDTDVLRRVSLFEGLSDKQLASVQRGTEVRLGKGDYVKRAGD
                     PPDAFYVVIEGRIEWTSRMRGKDVFVQSLGPGEFWGHEQLLTGKPYPANGRAVTPVCL
                     YVLGADEFWRMLSDCPSILRTLVALVVERFGNLGEAQQHAKLVSLGTMAAGLVHELNN
                     PAAAAGRSAREARETFRESSARAVRLGGLDMSPEERLVVAGLPEEVAVRAERTGEFDA
                     LERSDLEDEVALWLEDRGVEEAWEFSPTLVAAGLDATWLDDLEGRLPGGHVGDVLGWL
                     AFEVTGDELLREIGYASTRISELVGAVKTYSFMDKTASKEVDVHAGLNSTLIMLGHKL
                     KKGDVEVIRDYEKNLPPVCGHGGELNQVWTNLLDNAIDAVDGRGRITVRTASENGRVL
                     VEIGDDGPGIPEEARERIFEPFYTTKDVGKGTGLGLDISHRVVVEDHSGDIRV"
BASE COUNT          889 a         1521 c         1618 g          790 t
ORIGIN      
        1 gtaaaggaga gccatggcac agacgatggg catccaccac gtgaccgcca tctccggcga
       61 gccgcagcgc aacgtggact tctacgccgg tgttctgggg ctcaggctgg tcaagaagac
      121 cgtcaacttc gatgacccgg agacctacca cctgtactac ggggacgggg ggggcaatcc
      181 gggtacgatc atgaccttct tcccctgggc gcacgcctcc ggagggcgca taggagcggg
      241 acagctcatc gtaacctcgt tctccgtccc tatctcctcg ctcggctact ggacggagcg
      301 gctggtcgaa ggaggcgtac gtttcgagcg accgcgcgac cgcttcgggg ataccgtgct
      361 caccttcgag gaccccgacc gactccgcat cgagctggtc gccaccgagg acgcaagacc
      421 cggttggacc gggggtaccg ttcccgagga gcactccgtc aggggcttcc accacgtcgc
      481 cctcgccgtc gaggacatcg agagcacggc gggcctgatg accggggtgc tcggtttcgg
      541 gcaggtagac gagaccgagg gaaggatcag gctttcggcg ggcgagggtg ggccggggaa
      601 cacggtggac gtgatcgggg caaccggctt tccgcgcggg agcatgggcg tcgggacggt
      661 acaccacgtg gccttccggg taccagacga ggagactcag ctcgcccttc gggaggaagt
      721 ctctggtctc ggctacaacg ttacgccggt actcgacagg aactacttcc gctcaatcta
      781 cttccgcgag cccggcggcg tacttttcga gatcgccacc gaccctcccg gcttcgccgt
      841 ggacgaggac gaagcggagc tcggcaccca cctcaagctc ccgccctggc tcgagaccag
      901 acgagagagg ctcgaagagg ttctgccgcc cctggtggtc ccggagggag agagcgcatg
      961 acccccgacc tcggcttcga ccaccgctac ctgcccgctg gcgacagggg gggcgcaacg
     1021 ctccttctcc tccacggtac gggcggcaac gaggaggacc tgatccccct cggccaggag
     1081 cttctcccgg gcgccgccat cctctccccc cgcggcaagg tctccgagca tggcgccccc
     1141 cgcttcttcc gccggcttgc cgagggtgtc ttcgaccatg aagacctgct gtttcgcacc
     1201 cacgagctcg ccgcttttgt cgaggccgca gcagaagagt atggcttcga tcgttcgaag
     1261 atcattgccg tcggttactc caacggggcc aacgtggcag caagcaccat cttgctccat
     1321 cctggtctct tgcgggccgc ggtgctcttc agggccatgg tcccattcga gcccgaatcg
     1381 gcgccggatc tcaccggcat gcccgtcttc ctggccgcgg gcaggatgga ccgcatgatc
     1441 cccccggaca acacccagcg cctcgctgag atcctgaccg gggccggcgc cgacgtggac
     1501 ctccgctggc gcaacgtcgg ccaccctctg acctacgagg aggtcggcga ggccggagaa
     1561 tggatcgcaa gggtactgcc agggctagac acgtaggctg gcaccagtaa cgtccagaat
     1621 acccacacca agattagctg ctcgctaaaa gcgcgaacgg cggtgggcca ggctctctga
     1681 gtctcgacct ggggcgacct acccggatgg ccaggttgcc agggtgttag aatttttcga
     1741 ggacgtccgg aggggcatgc gggcgctatt tgaggggcgg aacaaccatt taacaatcgc
     1801 atttgaaggg cggatgacat ggcgaagccg gtgatactgg cggtggacga cgacccgcag
     1861 gtgctaaggg ccgtggagcg ggatctcaga cgccggtacg cgcgcgagta cagggtactt
     1921 cgcgcggact cgggggaatc ggccctcgac accctgggca ggctcaagct acggggagat
     1981 cccgtcgcgc tgtttctggt agaccagcgg atgcccagga tgaccggtgt ccagtttctg
     2041 gaggaggcca tagagcggta tccagaggcc aagcgggccc tgctcacggc atattcggac
     2101 accgaggccg cgatacgcgc tatcaacgag gtcggtctcg actactacct ccagaagccc
     2161 tgggacccgc cggatcagaa cctctatccc aacctcgacg acatgctggg cgactggcgg
     2221 gcagactaca gaccgccgtt cgagggaatc agggtcgtcg gagaccgctg gtcccccggc
     2281 tcgcaccgca cgcgcgattt cctggcccgc aaccgcgtgc cctaccgctg gctcgacgta
     2341 gagggctcga aggaggcgga ggagattctc gagatcacgg accatggcac accggcgctg
     2401 cctctcgtgg tctacgagga cggcacgtac gcagaagcgc ccgagaaccg ggagatagca
     2461 gagaagatag gtcttcaaac ccgcgccgaa caggagttct acgatctcct catcataggc
     2521 ggtggaccgt caggcctggc cgccgcggtc tacggcgcct cagaaggact gaagacggtg
     2581 ctcgtagaac gggaggcccc cggcggtcag gccggtacca gcagccgcat cgagaactat
     2641 ctcggttttc caaaggggct ttcgggcgga gatctggcgc gcagggcggt ggaccaggca
     2701 cggcgtttcg aggtggagat actcacgccg caggaggtcg cttcggtgcg tgtggaggat
     2761 ccctaccgca tcgtgacgct ctcggacggg acggagatct cttgcttcgc cctgatcatc
     2821 accacgggcg tctcctaccg caaactaaac gcgcccggca tcgagcgcct gacgggacgc
     2881 ggcgtttact acggcgcggc ccagaccgag gccctctcct gccgggacga ggacgtttac
     2941 atagtcgggg gcgccaattc ggccgggcaa gccgcgatgt tcttctccga caaagcccga
     3001 accgtatctc tggtgtgcag gggcgacgac ctgagagccg gcatgtcgga ttacctggtg
     3061 cgtcagatcg aagcgacgga caacatcgag gtgcttctgg ggaccacggt agcctccgcg
     3121 gacggcgaag accacctgga gaatctaacg ctaaagcacg ctgagaccgg caagtcggag
     3181 accgtgacgg cgcattcgct gttcatcttt atcggggctg cgccgatgac ggggtggctc
     3241 gacggtctag tggaacgcga cgagcggggt tttatcctct cgggcccaga tctcctgcga
     3301 gacggcaaga ggccagaggg ctggcgcccc cagagggacc cgttcttgct cgagaccagc
     3361 gtacccggta tcttcgtcgc cggggacgtc aggcacggtt cgatcaagcg ctgcgcctcg
     3421 gcggtcggag aaggttctat cgccgtgcag ttcgtccatc gctatctgaa cgaggtgtag
     3481 agatgagcgg cgacaccgac gtgctgcgcc gggtctcgct cttcgagggc ctgtccgaca
     3541 agcagctcgc ctccgtgcag cggggtacag aggttcgtct ggggaagggt gactatgtca
     3601 agagggccgg agatcctccc gacgccttct acgtcgtgat cgagggccgg atcgaatgga
     3661 cgagcaggat gcgagggaag gacgttttcg tgcagagcct gggaccagga gagttctggg
     3721 gccacgagca attgcttacg ggcaagccct acccggcgaa cgggcgtgcc gtgacccccg
     3781 tctgcctcta cgtgctcggg gccgacgagt tctggcggat gctctcggat tgtccctcga
     3841 tcctgcgcac cctcgtggcc ctcgtggtcg aacgcttcgg caacctgggg gaggctcaac
     3901 aacacgccaa gctcgtctcc ctggggacta tggccgccgg cctggtccac gagttgaaca
     3961 acccggccgc ggccgcgggg cgtagcgccc gcgaggccag agaaacgttc cgtgaatcct
     4021 cggcgagggc cgtcaggctc ggtggactcg acatgagccc cgaagaacga ctcgtcgtcg
     4081 cggggttgcc tgaggaggtg gcggtccgcg ccgaacggac gggggagttc gacgcactgg
     4141 agagaagcga cctcgaagac gaggtggcgc tctggctgga agaccgcggg gtcgaggagg
     4201 cgtgggagtt ctcccccacc ctcgtcgccg caggcctcga cgcgacgtgg ctcgacgacc
     4261 tcgagggccg tctccccggt ggccacgtcg gcgacgtttt gggctggctc gccttcgagg
     4321 tgacgggcga cgagttgttg cgcgagattg gttatgcctc gacccgcatc tccgagctcg
     4381 tgggggccgt caagacttac tctttcatgg acaagacggc ctccaaagag gtcgacgtcc
     4441 acgccgggtt gaacagcacc ctcatcatgc tcggccacaa gctgaaaaag ggcgacgtcg
     4501 aggtgatccg tgattacgag aagaaccttc cccccgtctg cgggcacggc ggcgagctga
     4561 accaggtatg gaccaacctc ctggacaacg ccatagacgc cgtcgacggg agaggccgca
     4621 tcacggtaag gaccgccagc gagaacggca gagtcctcgt agagatcggc gacgacggac
     4681 ccggcatccc agaggaggcg cgggagcgga tattcgagcc cttctacacg acgaaggacg
     4741 tgggcaaggg gacggggctc ggtctggaca tttcccaccg cgttgtcgtc gaagaccaca
     4801 gcggcgacat ccgcgtcc
//
LOCUS       DBNGVD010000408         3581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_462789, whole genome shotgun sequence.
ACCESSION   DBNGVD010000408 DBNGVD010000000
VERSION     DBNGVD010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_462789"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="AC16CM_10990"
     CDS             <1..939
                     /locus_tag="AC16CM_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3140401.1"
                     /translation="DRPDPPEGPGGGASQPDEGRSVGERSPAERGGEPSEARTGAGER
                     SHSASKPFEPVRIEAPEKGTGGPLGRRSRVVGGAGHPVGSRQDGENPGDVALAATVRA
                     AAPHQMARGRAGTGLEVRPHDLRQHVREGREGNLILFLVDASGSMAARSRMSAVKGAV
                     LSLLTDAYQRRDKVALISFRGEGARVLLPPTSSVELAAARLQDLPTGGRTPLAAGIDK
                     ASEVLKRESLRDQERRPLLVVLTDGRQTAGSDPTVAAARLRALGAAAFVVDTEEGYVR
                     LGMAGELAGALGARLLRLEELRADSLVDLVERRRVA"
     gene            936..1517
                     /gene="cobO"
                     /locus_tag="AC16CM_10995"
     CDS             936..1517
                     /gene="cobO"
                     /locus_tag="AC16CM_10995"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3140402.1"
                     /translation="MSDARETREPRERRLRKRSNRERPLLIVNTGHGKGKSTASFGLM
                     LRGWARGYRIGVYQFVKSGKWKVGEHQAALELGNIDWFKMGDGWTWTSRDLVESADKA
                     REGWEEVKRRLADETYDMLLLDEFTYPMKFGWVDTGEVVEVLKNRPGFQHIIITGRDA
                     PPELVEISDLVSEVNKVKHPFDEGYRGQKGIEW"
     gene            1511..2872
                     /locus_tag="AC16CM_11000"
     CDS             1511..2872
                     /locus_tag="AC16CM_11000"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR3140403.1"
                     /translation="MVDVPRIVVAGTHSGVGKTTVASGLMSALSSRGLRVAPFKVGPD
                     FIDPSYHTLAAGRPGRNLDAFLSGPERIGPLFAHGARGADVAIVEGVMGLFDGKSGGD
                     ELASTAHVAKLLDAPVVLVVDARAMSRSVAAMVHGYRTFDPHLKVLGVILNRVGSRTH
                     ENMLREALKPLHVPVLGVMYRDDEIRTPDRHLGLVPVAERRAEAERSLEALGTVISGS
                     VNLDEVLKLARSSESLHVEPWSPGSTEAGFAGSVQVAVAAGPSFSFMYRENLELLEAA
                     GAEVVEFDPTAGEALPAGTDALYLGGGFPEAYAGALSANVGMRESVRVFALGGRPVVA
                     ECGGLLYLSQELDGHPMCGVLDATARMTRRLTLGYREAVALADSPLAEAGTGVRGHEF
                     HYSAIEPGSGERPAWELADRGPEGFVVGGVHASYLHTHWAATPELPRRLVRAARGAVE
                     AAV"
     gene            2869..>3581
                     /gene="cobI"
                     /locus_tag="AC16CM_11005"
     CDS             2869..>3581
                     /gene="cobI"
                     /locus_tag="AC16CM_11005"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR3140404.1"
                     /translation="MSGRLVGVGVGPGDPELLTVKGLRVLREADAVFVPVADTGEVGR
                     AEATVREYLEVEGISRLLFALSDDPEARERNWSNAANEVSKHLENGETCAFATIGDPN
                     VYSTFTYLARTVRKMEPHVEVETVPGITAMQDLASRSGTVLLEGDGRLALLPFTAGEK
                     PLREALAGFETVVCYKGGSRLGEVLRVAEAEGRLEDAVYGARLGIEGEEISPAGEMIG
                     RDGTYLSTVVFTGGGSGLG"
BASE COUNT          598 a         1083 c         1330 g          570 t
ORIGIN      
        1 gatcgccccg acccgcccga aggccccggt ggtggggcat ctcagcccga cgagggacgg
       61 agcgtgggcg agcgttcgcc ggccgagcgg ggtggggagc cttccgaggc gcgtacgggg
      121 gccggcgagc ggagccattc ggcgtcgaag ccgttcgagc cggtacgcat cgaggcgccg
      181 gagaagggca cgggagggcc gctcggtcgc aggagccgcg ttgtgggcgg ggccgggcat
      241 cccgtcggga gcaggcagga cggtgagaac ccgggggacg tggccctcgc ggcgacggtg
      301 cgggcggcgg ccccgcacca gatggctcgg ggaagggctg ggaccgggct cgaggttcgt
      361 ccgcacgacc ttcgccagca cgtcagggag ggaagagagg gaaacctcat cctcttcctc
      421 gtcgacgcca gcgggtcgat ggcggcccgc agccgaatgt ccgccgtgaa gggagccgtc
      481 ctctcgttgc tcaccgacgc ctaccagcgg cgcgacaagg ttgccctgat ctccttccgc
      541 ggcgaaggtg cgcgggtact tcttcccccg acgtccagcg tcgagctggc cgccgcgcgt
      601 ttgcaggacc tcccaaccgg gggacgtaca cccctggcgg cagggatcga taaggcctcg
      661 gaggtgctga agcgggagag cctgcgtgac caggagcgca ggccgctgct cgtcgttctg
      721 acggacggac gccagacggc tgggtcggac ccgacggtgg ccgccgcacg gttgcgtgcc
      781 ctgggagcgg ctgcgttcgt ggtagatacg gaggaagggt atgtcaggct cgggatggcg
      841 ggtgagcttg ccggggcgct cggggcgcgt cttctgcgcc tcgaagagct cagggccgat
      901 tcgctggtgg atctggtaga gaggagaagg gtggcttgag cgacgcgcgg gagaccaggg
      961 agccgagaga gcgacggctc aggaagaggt ccaacaggga gaggccgctc ctcatcgtca
     1021 atactggcca cggcaagggc aagtccacgg cctcgttcgg gctcatgctg aggggatggg
     1081 cccgcggcta caggataggc gtctaccagt tcgtgaagag cggcaagtgg aaggtgggcg
     1141 agcatcaggc cgcgctggag ttgggcaaca tcgactggtt caagatgggc gacggttgga
     1201 cctggacctc tagagacctc gtggagagcg cggacaaggc ccgtgagggc tgggaggagg
     1261 tcaaacgccg cctcgccgac gagacctacg atatgctgct gctcgacgag ttcacctacc
     1321 cgatgaagtt tggctgggtt gacacgggtg aggtggtaga ggtcctgaag aaccgccccg
     1381 gctttcagca catcataatc accggacgcg acgccccgcc ggagctggta gagatctccg
     1441 acctcgtcag cgaggtaaac aaggtgaaac accccttcga tgagggttat cgtgggcaga
     1501 aaggcatcga gtggtagacg ttccacgcat agtcgtcgcg ggcacccact ctggcgtcgg
     1561 aaagacgacc gtcgcctcgg gcctgatgtc tgcgctctct tccaggggcc tgcgcgtcgc
     1621 ccccttcaag gtcggcccgg actttataga cccctcctac cacaccctcg cggctggccg
     1681 cccgggccgc aacctcgatg ccttcctgtc ggggccagag cggatcggcc cgctcttcgc
     1741 ccacggagcc aggggcgcgg acgtggccat cgtcgaaggc gtcatgggcc tcttcgacgg
     1801 caagagcggc ggggacgagc tggcctcgac cgcccacgta gccaaactgc tcgatgcgcc
     1861 ggtcgtgctc gtcgtcgacg cgagggcgat gtcccgttcc gtggcagcca tggtccacgg
     1921 ttacaggacc ttcgaccccc acttgaaggt cttgggcgtc atcctcaacc gcgttggctc
     1981 caggacccac gagaacatgc tccgcgaggc cctgaaaccc ctgcatgtcc ccgttctggg
     2041 cgtcatgtac cgcgacgacg aaatacgaac gcccgacagg cacctcggcc tcgttcccgt
     2101 ggccgagcgc cgcgccgagg ctgaacgctc cctcgaggct cttggaacgg tgatctccgg
     2161 ttccgtaaac ctcgacgaag tattgaagct cgcccgctca tccgaatcgc tgcacgtgga
     2221 gccctggagt cccggatcca cagaggcggg tttcgccggc agcgtacagg tcgccgtggc
     2281 cgcagggcca tctttcagct ttatgtacag ggagaacctg gagttgctgg aggccgctgg
     2341 tgccgaggtc gtagagttcg acccgacggc cggggaggcc ctaccggctg gaacggacgc
     2401 gctctacctg gggggcggat ttcccgaggc gtacgccggt gcgctctccg ccaacgtagg
     2461 gatgagggag agcgtacgcg tcttcgccct tggcggacgc ccggtagtgg ccgagtgcgg
     2521 ggggctgctg tacctgagcc aggagctaga cgggcatccc atgtgcgggg tcctggacgc
     2581 cacggccagg atgaccagga ggctcaccct cggatatagg gaggccgtcg cgctcgcgga
     2641 ttcgccgctc gctgaggctg gcaccggggt caggggccac gagtttcatt actccgccat
     2701 cgagccaggg tccggcgagc ggccggcctg ggagctagcg gaccggggtc ccgaagggtt
     2761 cgtggtcggc ggcgtgcacg ccagctatct gcacacgcac tgggccgcga cgccggagct
     2821 gccgcgcagg ctcgttcggg ccgcgcgcgg agcggtggag gccgcggtat gagcgggcgc
     2881 ctcgtcgggg tgggggtagg gccaggagac cccgagttgt tgacggtcaa agggctcagg
     2941 gtgttgaggg aggccgacgc ggtcttcgtg ccggtcgccg atacggggga ggtcggaagg
     3001 gccgaggcca ccgttcgcga gtacctggaa gtggagggga tctcccgcct cctcttcgcc
     3061 ctctcggacg acccggaggc ccgggaacgt aactggtcca acgcggcaaa cgaggtctcg
     3121 aagcacctgg agaacggcga gacctgcgcc ttcgcgacca tcggtgaccc gaatgtatat
     3181 tcgactttta cctacctggc gcgcaccgta agaaagatgg aacctcacgt ggaggtagag
     3241 accgtgccgg ggattactgc catgcaggat cttgcatcgc gcagcggcac cgtattgctc
     3301 gaaggcgacg gccggctcgc gctgttgcct ttcacggccg gtgagaagcc gctcagggag
     3361 gcactcgcgg gcttcgagac ggtggtgtgc tacaagggtg gtagtcggtt gggagaggtg
     3421 ttgcgggtcg cggaggcgga gggccgattg gaggacgccg tgtacggtgc gaggctcggc
     3481 atcgagggtg aggagatctc gccagcaggg gagatgatcg gcagggatgg tacttacctg
     3541 tccaccgtgg tctttacagg aggagggtcc gggcttgggt a
//
LOCUS       DBNGVD010000409         5278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1373485, whole genome shotgun sequence.
ACCESSION   DBNGVD010000409 DBNGVD010000000
VERSION     DBNGVD010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1373485"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="AC16CM_11010"
     CDS             <1..1176
                     /locus_tag="AC16CM_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HIR3140405.1"
                     /translation="LAFAERVMKFVDDEAWEESRNLAEERGVMPNFEGSRHEMRGDRV
                     RNATVTTIAPTGTISMIAGCSGGIEPLFAVAFMRRQADMEMPDVNPEFVKLARERGFY
                     SEELMKKIAEHGSVRDVEEVPDDVRSVWVTSHDITSDWHVKMQAAFQKYTSMGISKTI
                     NLPYEASVEDVEDAYRQAFATNCKGIAVYRDGSRDAQVLSTGKTGGEKPTMTPASPEA
                     PTAPPVTPPATEGKGQTFSSLAEARNGRPRTLTGVTKKIRTGHGSMYVTMNDDEFGPR
                     ECFVVLGKPGGTAAAFSDALGRMITMALTHGADPAEVVHQLRGIQDGHPAGVGPNAVL
                     SVPDGVARAMSEHYLIEDSLPDTRELPIIGACPDCGSGLTRQEGCVVCHSCGYSKCS"
     gene            1383..1583
                     /locus_tag="AC16CM_11015"
     CDS             1383..1583
                     /locus_tag="AC16CM_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140406.1"
                     /translation="MKIRLTQNVPKYGRLSKGIVVEAEPVAHHPDLMRVKDFGAFENG
                     ALARSWELVVSDEEIEALLREG"
     gene            complement(1642..2574)
                     /locus_tag="AC16CM_11020"
     CDS             complement(1642..2574)
                     /locus_tag="AC16CM_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3140407.1"
                     /translation="MSVTNGTSARRYAWLSIAAAVVTIGLKLGAYYLTGSVGLFSDAA
                     ESVVNLVAAVAALWALTLAMRPPDEEHAFGHSKAEYFSSGLESALIIIAAVWIGVTAW
                     GRLLDPQPLQNLGLGLSVTLVAAAVNGGVALVLLRAGRRLRSITLRADAQHLLTDVWT
                     SLGVVLGIVMVYLTGWLVLDPIIGLIVAANIVWTGVRLFRDTAQGLLDRALPPDEQKL
                     ITGVLSLYESRGIRFHALRTRAAGQRRFVSMHVLVPGRWTVKRGHDLSEELEGELANA
                     LQGNTTFFIHIEPSEDPVSFQDQTLDRDRSNTRS"
     gene            complement(2634..3893)
                     /locus_tag="AC16CM_11025"
     CDS             complement(2634..3893)
                     /locus_tag="AC16CM_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140408.1"
                     /translation="MSSRAPTSASTLRGRWLVAVRAAWVGIATLAFGVLVASIPGYVS
                     DILQLGRASWMGAPVEAPAALVFTLDLLGVLASVTAALLCLTLGIVLFWRRSNDWMVV
                     FISAYLLAYGTVFAGPLEWAQDYYPWLPPLAISVVQPLLLTVPTVALIILFPDGRVVS
                     RGARWLGISSVLLVIAILFLPPAFGYALWPVILLGALSTQIYRYQRVSTPTERQQTKW
                     VVFGILLWLVLMGLLGIPYALVTSMPPGSVLPWWALAGSTGWWLTLTIVPVSLSIAVL
                     RYRLYDIDVVINRALVYGSLTAMLLLVYFGGVATTQALLRVLTGHERLSQLTVVVSTL
                     AIAALFDPLRRRIQAFIDRRFYRRKYDARKTLEGFSARLRDETNLDSLNTELVSVVQE
                     TMQPAHVSVWLRPDATPEDRRRASGRW"
     gene            4480..4569
                     /locus_tag="AC16CM_11030"
     tRNA            4480..4569
                     /locus_tag="AC16CM_11030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4514..4516,aa:Ser,seq:cga)
     gene            4885..>5278
                     /locus_tag="AC16CM_11035"
     CDS             4885..>5278
                     /locus_tag="AC16CM_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140409.1"
                     /translation="MRVRKGGTGWDAVLFTAGFWVLAGSLWLQVGYMGAALGRISTES
                     MEVHADFDSFWRSARAMLRGQDIYDTGVALVNLNPPVWTVLISPLGLLGPLTAYRVFV
                     LFSLILAIGYLVWTVEELRLRPGWAAVGV"
BASE COUNT         1016 a         1672 c         1724 g          866 t
ORIGIN      
        1 ctcgccttcg ccgagagggt gatgaagttc gtggacgacg aggcgtggga ggagagccgc
       61 aacctcgccg aggagcgcgg cgtgatgccg aacttcgaag gctcccgtca cgagatgcgc
      121 ggcgacaggg tccgcaacgc gaccgtcacc actatcgccc ccacgggcac catctccatg
      181 atcgccggct gctctggcgg catcgagccg ctcttcgccg tggcgttcat gcgccgacag
      241 gccgacatgg agatgcccga cgtgaacccc gagttcgtca agctcgccag ggagcgcggc
      301 ttctactccg aggagttgat gaagaagatc gccgagcacg gctccgtgcg cgacgtcgag
      361 gaagtacccg acgacgtgcg cagcgtgtgg gtcacctcgc acgacatcac gagcgattgg
      421 cacgtcaaga tgcaggcggc gttccagaag tacacctcga tgggcatctc aaagaccatc
      481 aacctgccct acgaggcgag cgtggaggac gtggaggacg cctaccgcca ggccttcgca
      541 acaaactgca agggcatagc cgtctacagg gacggctcgc gcgacgctca ggtgttgtcc
      601 acgggcaaga ccggtgggga gaagccgacc atgacgccgg cctcgcccga ggcgccgacc
      661 gcgccgcccg tcacaccgcc ggccacggag ggcaaggggc agaccttctc ctcgctcgcc
      721 gaggcacgaa acggcaggcc gcgcaccctc acgggcgtga ccaagaagat ccggaccggc
      781 cacggctcca tgtacgtaac catgaacgac gacgagttcg ggccaaggga gtgcttcgtc
      841 gtactcggca agcccggcgg cacggccgcg gcgttctcgg acgcccttgg gcgtatgatc
      901 acgatggccc tcacccacgg cgccgatccg gccgaggtgg tccaccagct ccgcggcatc
      961 caggacggcc accctgcggg cgtcggacca aacgcggttc tgtctgttcc cgacggcgta
     1021 gcacgggcca tgtccgagca ttacctgatc gaggacagcc tcccggacac ccgcgagctg
     1081 ccgatcatcg gcgcctgtcc cgactgcggc agcggcctga cccgccagga gggctgcgtg
     1141 gtttgccact cctgcgggta ctcgaagtgt agctagagag aacagacgcg gactgcgggg
     1201 gatgggccta ttcggcccgt cccctttcgt gttggtgtcg gttcttctct agccttcagc
     1261 cttcagcttt ttgtctgggc ttgcacgttt ctcgtgtttg atgggtcggg cttcggacgg
     1321 aaatgtaagc gaaagaacaa tatcgggtcg cgcccgcgtg cacaatgtcg ggcagagcaa
     1381 tcatgaagat tcgtcttaca caaaatgttc ccaagtacgg ccgcctcagc aaaggcatcg
     1441 tcgtggaggc cgagcccgtg gcacaccatc ccgaccttat gcgggtgaag gacttcgggg
     1501 ctttcgagaa cggggcgcta gcgcggagtt gggagctggt cgtgtcggac gaggagatcg
     1561 aggccctcct gagggagggc tagtcgccag cttctccacc gtgccggcga cgtttacccg
     1621 aatgtccgac gggacctgca ctcatgaccg ggtgttagac cggtctctgt ccagcgtctg
     1681 atcctggaac gagaccgggt cttccgaggg ttctatgtgg atgaagaaag tggtgttccc
     1741 ctgcagggca ttcgccagct caccctccag ttcctctgac aggtcatggc cccgcttcac
     1801 ggtccagcgg ccaggaacga gcacgtgcat ggagacgaaa cggcgctggc cggccgcgcg
     1861 cgtgcgcagg gcgtggaagc ggattccccg gctctcgtag agggagagga cgccggtgat
     1921 gagtttctgt tcatcgggtg gcagtgcccg gtccagcagc ccctgcgcgg tgtcgcggaa
     1981 cagccgcacg cccgtccaga cgatgttcgc ggccactatg agcccgatga tggggtcgag
     2041 cacgagccac ccggtcaggt acaccatcac gatgccaagc acgacgccga gcgaggtcca
     2101 cacatcggtg agcaggtgct gggcgtcagc ccggagcgtg atcgaacgca gcctgcggcc
     2161 ggcgcgcaag agcacgagcg cgacgccccc gttgaccgca gcagccacca gcgtgacgga
     2221 gaggcccagc cccaggttct ggagcggctg cgggtcaagc agacgtcccc aggccgtaac
     2281 cccgatccag accgccgcga tgatgatgag cgcgctctcc agcccgctcg agaaatactc
     2341 ggccttgcta tggccgaagg cgtgctcctc gtccggaggg cgcatcgcca gggtcaacgc
     2401 ccacagcgcg gccaccgccg cgacgaggtt gacgacggac tcggcggcgt cggagaagag
     2461 ccccaccgaa cccgtaaggt agtaggcccc gagcttcagc ccgatggtga cgaccgcggc
     2521 cgcgatcgag agccaggcgt agcgccgcgc agaggtgccg ttcgtgacgc tcacctcggt
     2581 gatcgcggcc caaccgtcgc aagtttcgat atcgcagccg gcacgctaac gttctaccat
     2641 cttcccgatg cgcgtcgccg atcttcgggg gtggcatccg gacgcagcca cacggaaacg
     2701 tgcgcaggct gcatcgtctc ctgcaccaca gagaccagct cggtgttcag gctgtccagg
     2761 ttcgtctcgt cgcgcaaccg ggccgagaaa ccctccagcg tctttctagc gtcgtacttg
     2821 cgcctgtaga agcgtctgtc tatgaacgcc tggatgcggc gtctcagggg gtcgaaaagc
     2881 gcagcgatgg cgagcgtgga gaccacgacg gtgagttggg agagacgctc atggccggtg
     2941 aggactcgaa ggagcgcctg ggtcgtggcc acgcccccga agtagaccag caacagcatc
     3001 gccgtaagcg aaccgtagac gagggcgcgg tttatgacga cgtcgatgtc atagagccgg
     3061 tagcggagca cggctatgct cagcgagacc ggcactatgg tcaacgtcag ccaccagccg
     3121 gtgctcccgg cgagggccca ccagggaagg acgctgcccg ggggcatgga cgttaccaac
     3181 gcgtagggta tgccgagcaa gcccatcagc accaaccaca gcaggatgcc gaacaccacc
     3241 cacttggttt gttgccgctc ggtcggcgta gacactcgtt ggtaccgata gatctgtgta
     3301 gagagcgcgc ccaacaggat tacgggccac agggcatacc cgaacgcggg cggcaagaac
     3361 agaatcgcta tcaccagcag aacagacgag atacccaacc agcgggcccc gcgcgacacg
     3421 acccgtccgt ccggaaacag gatgatgagc gccaccgttg gcaccgtgag aagcagtggc
     3481 tggaccacgg atatggctaa cggaggcaac cagggataat agtcctgcgc ccactccagc
     3541 gggccggcaa aaacggtccc gtaggcaagg aggtacgcgg agataaacac gaccatccag
     3601 tcgttggatc tcctccagaa gagcacgatc ccaagggtca gacacagcaa cgccgccgta
     3661 acggatgcca ggaccccaag caggtccaac gtgaacacca acgctgcagg agcctctacg
     3721 ggagcaccca tccaactggc ccgaccgagc tggaggatgt cggagacgta gccggggata
     3781 gaggcgacca gaacgccgaa agccagcgtg gcgataccca cccatgccgc gcggaccgcg
     3841 actagccacc gaccacgcag ggtcgaggcc gatgtcggag cacgcgagct catcccgatc
     3901 gctgaccgta ccttttcatg gaccgtctgg ttaagccctc cactctactt ccccgccgta
     3961 ccccttatca tttgctgccg gccctccacc ctggagcgta ccctcgacga aacgcgtagt
     4021 ctccctgcgt ccggtctcaa cacccctttc gagaccgggt accccaagca ctctttcatc
     4081 ccaccatacc gacccgtcgt ctgccaaccg ttacggcaaa ggttacggcc cgacaaaccc
     4141 tcgggctacg acccgggatc gaactcggtc cgatgtgcct ggagatttcc gggagaaccc
     4201 gaccatcacg ccccgacaga tactgtatat ctaaagcgaa aggttagggt tcatcggaga
     4261 aaatatcatc ttcggcctgg ctcaatcgtg acctggaaaa ccgttatttg caggtattgc
     4321 gtctcctggt tggtcgaact tcgagcccgc gcgggctgga cagggccagg cgaaacgtcc
     4381 ggccgagcag ctcagaagca gaggtatagg gtcgggctga cggtcgcgag taccgtaatg
     4441 cggttgaagt ggcgagacgc agtggataca ctcagccgcg gagaggtggc agagtggttg
     4501 aatgcggcgg tctcgaaaac cgttacgtcg gtaaccccgg cgtcgggggt tcgaatcccc
     4561 ccctctccgc aggggcgcgc cgtgcggcgc gcccgcacgc tggcttcgct tcgctacgcc
     4621 agcttgtcag cacgctccag cctagcggct gtcgcttgtc ggcaacgctt cgcccggctt
     4681 caccgggctc gctttcggcc agtcgtctcg gattgctacg gcaagccagg aatctatagt
     4741 ccctacgcac ctgtacactg tgtgcgcgag ggtagggggc cgcagccttg ttggctgaca
     4801 gcgaagccct gaaagggctg aagcgtgctg acaagccgcg aagcggcgtg ctgacaagcg
     4861 gaggcggagc cggagcgtgc tgacatgcgt gtaaggaagg gcggtactgg ctgggacgcc
     4921 gtcttgttca cggccgggtt ctgggtgctt gcgggttcgc tctggttgca ggttgggtac
     4981 atgggggcgg cgctcggaag gatctcgact gagtcaatgg aggtccacgc ggacttcgac
     5041 agcttctggc gttcggcgcg ggccatgctc cgggggcagg atatctatga cacgggggtc
     5101 gcgctcgtga acctgaatcc ccccgtgtgg accgtgctca tctcgccgct agggctgctc
     5161 gggccgctca ccgcgtacag ggtgttcgtg ttgttctcgc tgatcttggc catcggttac
     5221 ctggtgtgga ccgtggagga actgcgttta cggccggggt gggcggcggt gggggtgg
//
LOCUS       DBNGVD010000410         5316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2465728, whole genome shotgun sequence.
ACCESSION   DBNGVD010000410 DBNGVD010000000
VERSION     DBNGVD010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2465728"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16CM_11040"
     CDS             <1..459
                     /locus_tag="AC16CM_11040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020135.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3140410.1"
                     /translation="LLVNSSFSGLGAISGLLYREVIADPVGKEAALAVWREGYDVGLA
                     QNITLDRVLGVPAEALVVRGPQDRGRADVALEVAMGYAGATKASMLQDLERGVKTEVD
                     VINGGVVERGREYGIETPYNERTVEIMHTMERGERSPGRDALVELGAVSG"
     gene            complement(571..1773)
                     /locus_tag="AC16CM_11045"
     CDS             complement(571..1773)
                     /locus_tag="AC16CM_11045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3140411.1"
                     /translation="MSLRKTVLSLASLAAALLLASGIALAAVPGGSAAGEKGAGPTNT
                     IAADAALDGALKSLVARHGGPPGAIAVVQRGKHREVHAFGVGNIETGRPMRADDHMRI
                     ASAAKAFSGGVALSLVGKGELSLNDTIGERLPELPDAWSEVTLRQLLNHTSGIPDFSL
                     DPDFREAVFASLKKAPPPEELLTYVYDEDLLFTPGSKYHYSNSDNIAVALMVEAATGK
                     SYERQLREQVYRPLGLEETSLPRGVYLERPYIHGYDNDPKDYPPEDYSKLIAAGWAWA
                     SGGIVSTPSDMNDFIRGYVGGDLFGRNVQTQQRRVFEGGSSEPPGPGYNSAGLALFRY
                     QTRCGTVWGHTGNTPGYTQFMAASPNGNRSVVVSINTQLTPNDGTLSAFKALRRVEGK
                     AVCAALAG"
     gene            complement(1957..2841)
                     /locus_tag="AC16CM_11050"
     CDS             complement(1957..2841)
                     /locus_tag="AC16CM_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140412.1"
                     /translation="MYRGKPQTPVIRSRKLKVYDAIEIAHLRLLPGEVETMPMRGHLV
                     NLHLSSSPHYLIQRRNERTHEGTKNPGVVDIIPTDTPALWSMDAASEHACMLIEDRFL
                     RRVAVEAGVNLDGFEVVPRFSTQDAQIEVLGLSLLSEMKSDGLGGKLYSESLANLLAL
                     HLLRVHSSLGRGSRRKLEHQNEREYGLSRRSLSEVNDYINDNLRRKLTLRELAGVVHI
                     SPHYFARSFKATTGTSPHQYVVSRRVERARSLLANSSLTITEVAIAVGFSSQSHLASH
                     VRRVLGVSPGDLRRQGLP"
     gene            complement(3406..3783)
                     /locus_tag="AC16CM_11055"
     CDS             complement(3406..3783)
                     /locus_tag="AC16CM_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140413.1"
                     /translation="MRKIMLILALVVMLVHLSAAAALAYHQLIQCKSVPCYGSGGDDK
                     ILERKGNGLYDKIIMRGGYDLVQANGYGNDTDVVKGGTGYDKINVADGDKLDTASGGA
                     GRDWCIVDARKETTASCARVTVR"
     gene            4229..4990
                     /locus_tag="AC16CM_11060"
     CDS             4229..4990
                     /locus_tag="AC16CM_11060"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3140414.1"
                     /translation="MRVIGAGFGRTGTTSLKAALGELGFGPSYTLGEVFANPEHVARW
                     ETARRGGEVDWEGFLAGYEVAVDWPACSFYAELMQAFPDAPVILTIRDETAWYESTRS
                     TIHGLRRLTTGPLPLRAAFALASPFAPGPAGTVRLADRLVWEDTFHGRFEDRDYAMEA
                     FERHNETVRRRVPKGRLLVFDVRQGWEPLCHFLGVEVPDTPFPHLNEAREMRRKLLGL
                     VALSAILPSLAVLTGVVATALLIRRTASLRHRRTP"
     gene            complement(5060..>5316)
                     /locus_tag="AC16CM_11065"
     CDS             complement(5060..>5316)
                     /locus_tag="AC16CM_11065"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3140415.1"
                     /translation="SADTAKRLKPAPAPYRMAARALGIPDRELRLVAAHAWDVAGALR
                     AGCAAAFVARQPYDPLVERPDVVGADLSEVADGIIATEQN"
BASE COUNT          885 a         1724 c         1696 g         1011 t
ORIGIN      
        1 ctactcgtca acagctcctt ctccggcctc ggcgccatct ccggtctgct ctaccgcgag
       61 gtcatcgccg accccgtggg aaaggaggct gcgctcgccg tctggcgcga aggatacgat
      121 gtgggcctgg cccagaacat cacgctggac cgggtcctcg gcgttcccgc cgaagcgctt
      181 gtagtccgtg gtccgcagga cagagggagg gcagacgtgg ccctcgaggt ggcgatgggc
      241 tacgccgggg cgaccaaggc ctcgatgctc caggaccttg agcgcggggt gaagaccgag
      301 gtagacgtca tcaacggcgg cgtggtcgag cggggaaggg agtacggtat cgagaccccc
      361 tacaacgagc ggaccgtcga gatcatgcac accatggaac gcggggaacg aagcccggga
      421 cgtgacgccc tggtggagct aggggccgtc tccggctgaa gcgcgtcccg gccggtgaac
      481 gtcctccgta cccacggaac catccgaata gagatgacgg aaagaggccg cccgcaagcg
      541 gcccctttcc gtcatctggc gcctttccta ctagcccgcc agtgcggcgc acacggcttt
      601 gccctcgacg cgacgtaaag ctttgaaggc gctgagtgta ccatcgttgg gggtaagctg
      661 cgtgttgatc gacaccacca ccgagcggtt cccgttgggg ctcgccgcca tgaactgggt
      721 gtagccgggg gtattaccgg tgtggcccca caccgtcccg cacctggtct ggtagcggaa
      781 gagggccagc ccagccgagt tgtacccggg gccggggggc tctgagcttc caccctcgaa
      841 gaccctgcgc tgctgcgtct gtacgttcct accaaagagg tcgcccccga cgtagccgcg
      901 gatgaaatcg ttcatgtccg agggcgtaga tacgatgccc ccggaggccc aggcccagcc
      961 cgccgctatt agcttgctgt aatcctcggg tggatagtct ttgggatcgt tgtcgtagcc
     1021 gtggatgtag ggtcgctcca gatagacgcc tcggggcagg ctggtctcct ccaggccgag
     1081 aggcctgtag acctgctcac ggagctgccg ctcgtagctc ttgcccgtgg ccgcctcgac
     1141 cataagcgcc acggctatgt tgtcggagtt ggagtagtgg tacttcgagc ccggcgtgaa
     1201 gagcagatcc tcgtcgtaga cataggtgag cagctcctcg ggcggtggcg ccttcttcag
     1261 gctggcgaag accgcttccc ggaagtcggg gtccagcgag aagtcgggga tgccgctggt
     1321 gtggttgagc agttgcctaa gggtaacctc cgaccaggcg tctggcagtt cgggcaggcg
     1381 ctcgccgatt gtgtcgttca gggagagctc gcccttgccg accagggaca gcgccacgcc
     1441 gccgctgaag gccttggccg cgctcgcgat gcgcatgtgg tcgtcggcac gcatgggccg
     1501 tccggtctct atgttgccca ccccgaaggc gtgtacctcc ctgtgcttgc cgcgctgcac
     1561 cacggcgatt gcccctggag ggccgccgtg cctggcgacc agcgatttca gggcgccatc
     1621 gagcgcggca tcggcagcta tggtattggt tggcccagcc cccttctccc cggcagcgct
     1681 gccgccggga actgcggcca gcgcaatccc gctagccagg agcaatgccg ccgccaacga
     1741 tgccagcgat agaacagtct tcctcaagct cactatcctt cctctagttc gacacccgga
     1801 gatctcccgg gccccactcc acacgaagag catcccgcat cgcgcggtcc acccggaggg
     1861 attatccgcc aactcgcttc gggttggcaa acagaatcct gccctttttg accaggatct
     1921 tgccctattt cagaagtatg cacgagatcg aaggcgctag ggtaggccct ggcgtcgcag
     1981 gtcgcccggg gagacgccta gcacgcgcct gacgtgggag gccagatggc tctggctcga
     2041 gaagccaacg gcaatcgcta cctcggtaat ggtcaggctg ctattcgcga ggaggctcct
     2101 cgcccgttcg acgcgcctgc ttacgacgta ctggtgcggt gaggttcctg tcgtggcctt
     2161 gaatgagcgg gcgaagtagt gggggcttat gtgcacgacc cctgctagct cccgaagcgt
     2221 cagcttgcgg cggaggttat cgtttatgta gtcgttgacc tcggagagag agcgtctcga
     2281 caggccatac tcgcgttcgt tctggtgctc gagctttcgc ctcgaaccac gtcccagcga
     2341 ggaatgcact cgcagcagat gcagcgctag caggttggct agcgactcgg agtacagttt
     2401 gccccccagg ccatctgact tcatctccga gagcaacgac agcccgagaa cctctatctg
     2461 cgcgtcttgg gtactgaagc gaggcacgac ctcgaaccca tcgaggttca cgcccgcctc
     2521 gacggccacc cgccggagga agcggtcttc gatgagcatg caagcgtgct cggaggccgc
     2581 gtccatgctc cagagggcag gcgtgtcggt cggtatgatg tccacgacgc ccgggttctt
     2641 ggtcccctcg tgtgtacgct cgttgcgacg ctgtatcagg tagtgtggtg agctgctcag
     2701 gtgcaggttg accaggtgtc cccgcatggg catggtctcg acctcgccgg gaaggagcct
     2761 gaggtgcgct atctcgatcg cgtcgtaaac cttgagtttc ctcgaccgta ttaccggcgt
     2821 ttgcggcttc cctctgtaca actcagcccg taaatcgtcc tccgggcgcc agtaacggtg
     2881 cccgtgtgaa cgatacgtgc tcaccatctt gttgtccttt cgtggccctt gccctcatct
     2941 cgcggcaaag cgagcaagca cggtccctaa cctcggtctg gataacaaaa ccgaagcata
     3001 ctcggtatgc ctcgctacgg caagccagta gtagccccgt agagcaccgt aatagtcgcc
     3061 gtgccagacg atcgggcagt accctggtcc ggaaactacc ggccctcgca agccagcccc
     3121 gtctgccccg atcgggcgca caacgcgatc gcgggcaagc acaaggacgg tgcggtgtac
     3181 cgagctggta cgccgccccg ttctcagaga gattcgcttt cgagccggtt acccggcgga
     3241 ggcctagctc gcccgtttcg agtgggcttt gaggctcgct tctgccacgt ggggcgcctc
     3301 ctttcccacc cgttgcgcgg atcgacgaac atttactggc ttgtccgtga ccatcctctg
     3361 accttgcggc ttaacttcgc tcgaccatag gggcgggggt gatctctagc ggaccgtcac
     3421 cctggcgcag ctcgccgttg tttccttcct ggcgtccacg atgcaccagt cgcgccccgc
     3481 tcccccagag gccgtgtcaa gcttgtcccc gtcggccacg ttgatcttgt cgtagcccgt
     3541 gccgcccttg accacgtcgg tgtcgttacc gtagccgttg gcctgcacga ggtcgtagcc
     3601 gcccctcatg atgatcttgt cgtagaggcc gttgcccttg cgctcgagga tcttgtcgtc
     3661 tccgccagat ccatagcagg gtacgctctt gcactggatc agctggtggt aggccagggc
     3721 cgccgccgca gacaggtgca cgagcatcac caccagggcc agaatcaaca ttatcttgcg
     3781 catagatctc ctattctccg tcaggtgggc gcggtcacac ccgacctgtg acctgctgat
     3841 ctactgaacc ggtcgcctgg gacgatcatc ggccaggaac ctcaccggtg cttcttccag
     3901 tgccaactcc tcttctcgtc tggacgtccg gtttgccggg cccatccacc acgagaagca
     3961 cccccgtggc gcagtacacg gaatggaatt attcgctagc ttggggcgga ctggcgaaca
     4021 gaaccttgct attggtgagc aagatcttgc tttttgggga ggtggactgc tggaacgaag
     4081 ccgctcgtgg caacgggtcg ttcgaaagag cacatctcgg aatgccccag ggtcttggga
     4141 catcgtcctg gtctccgatc ggagcagggt tgcacaggag gcaccgcctc gcgagcgcat
     4201 cccgagcgag tcggtgtaga gtgaccccat gagggtaata ggggccggct tcggccgcac
     4261 gggcacgacg tcgctcaagg cggcgctggg ggaattgggc ttcggcccga gctacacgct
     4321 gggcgaggtc ttcgccaacc cggagcacgt cgcgcgctgg gagaccgccc gccgcggggg
     4381 cgaggttgat tgggagggct tcctggctgg ctacgaggtg gcggtcgact ggccggcctg
     4441 ctcgttctac gcggagctca tgcaggcgtt tcccgatgcg cccgtgatcc tgaccatccg
     4501 agacgaaacc gcgtggtacg agagcacgcg ctcgaccatc cacgggctga ggaggctgac
     4561 cacgggaccc ctgccgttga gggctgcctt cgcgctggcc agccccttcg ccccgggccc
     4621 cgcgggcacc gtcaggctgg ccgaccggct ggtatgggag gacaccttcc acggtcgctt
     4681 cgaggacaga gactacgcta tggaggcctt cgagcgccac aacgagacgg tccgccgccg
     4741 cgtgccgaag gggaggcttc tggtcttcga cgtgcggcag ggctgggagc cgctctgcca
     4801 cttcctcggc gtcgaggtcc ccgacacccc attcccccac ctcaacgagg ccagggagat
     4861 gaggcgcaag ctgctcggtc tcgtcgccct ctccgccatc ttgccatcac tcgcagtact
     4921 gacaggcgtc gtcgcaacgg cgctcctcat tcggcgtacc gcgagcttgc ggcaccgccg
     4981 aaccccctga agagaggttt caagcttcgg gaaaattacc tgatgccttc ccccaggctc
     5041 acttctgcct cgaacgtcgt tagttctgtt cggtagctat gatgccgtcg gccacttcgg
     5101 agagatccgc gccaaccacg tcgggtctct cgacgagcgg gtcgtagggc tgcctggcca
     5161 cgaaggctgc tgcgcatcca gccctcaacg ccccggccac gtcccaggcg tgcgccgcga
     5221 ccaggcgcag ctcgcggtcc ggtataccga gtgcccgggc ggccatcctg tagggggctg
     5281 gtgccggctt caggcgtttt gccgtgtcgg cggaga
//
LOCUS       DBNGVD010000411         4577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4652925, whole genome shotgun sequence.
ACCESSION   DBNGVD010000411 DBNGVD010000000
VERSION     DBNGVD010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4652925"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="AC16CM_11070"
     CDS             complement(<1..722)
                     /locus_tag="AC16CM_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3140416.1"
                     /translation="MVSLFVALLTLAGCGGGGGAPESSTPPGTLEVALEAEPPELDPS
                     LSSAYVDRQVMASIYDRLVDLDENGKIVPMLAKSWNVSGDGRTYTFELEKGVKFHDGT
                     PFDAEAVKFNLERYQKADSVRSTEIEPVQSVEVVDDHTVRVELSEPFAPFLAVLTDRA
                     GIMASPEAIEKSDGRISKNPVGTGPFQFVERVRGDHITLEKNPDYWQDGLPKLNKVVY
                     NGIDDENVQYQNLQSGELDLIDS"
     gene            complement(945..2684)
                     /locus_tag="AC16CM_11075"
     CDS             complement(945..2684)
                     /locus_tag="AC16CM_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3140417.1"
                     /translation="MVASTHWLASSAGMAVLESGGNAFDAAVAAGLTLQVVEPHLNGP
                     GGEVPILLHSAEGGRTLVVAGQGPAPAAVSIDHFERLGLDLVPGTGLLAACVPGAFDA
                     WMLMLGEFGTLRLSEVMRYAIDYAGGGYPLVPQISATISGVEGLFRDEWPSSARVYLR
                     GSGVPRPGTLFSNPDLAATYRRILEEAEATTSNRDGQIEAARDVFYRGFVAEAIDRYA
                     SSETVMDTSGRRHTGLLKGDDLAAYAADLEEPATLDYHGYTVCKTGPWGQGPVFLQQL
                     ALLEGFDLTSMGHNTAEYVHTVVECAKLAFADREAHYGDPRFVDALHEPLLHPSYTAS
                     RRMLVGEDASYDLRPGTAGGRAPRLPDRSGISQDGAAGAGEPTVPRDDQGSGDTCHRD
                     VVDRHGNMVSATPSGGWLQSSPVVPGLGFPLGTRAQMFWLTAGLPNSLEGGKRPRTTL
                     TPSLALRDGEPYLAFGTPGGDQQDQWSLHFFLAHAHFGLNLQEAIDAPNFHTEHFPSS
                     FYPRAAAPGRIMVEDRIGPDVVAGLRARDHDVDVVDGWSLGRISAVSRDARDGSMRGA
                     ANPRGMQGYAVGR"
     gene            complement(2729..3592)
                     /locus_tag="AC16CM_11080"
     CDS             complement(2729..3592)
                     /locus_tag="AC16CM_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR3140418.1"
                     /translation="MRTDQIVATFSIVAFDPETDSLGVAVQSKFLAVGSVVPWARAGV
                     GAVATQAMANYNYGPRGLDLMAAGNSAVQTVEALTTADEEREHRQVGVVDARGRASTY
                     TGSECFDWAGGVTGEHYAAQCNILVGIETVEAMAGAYEEADGDLAARLLGALDAGQRA
                     GGDSRGKQSAALLVVREGGGYGGDNDRVLDLRVDDHPEPISELIRIRELHTLYFGETN
                     PDDVIAVDGDVRAQVAGALLRAGYLDGPPAGDDDLFGALRAYVRTENFEEREQRRGYL
                     DRAVFEHMKKG"
     gene            3850..>4577
                     /locus_tag="AC16CM_11085"
     CDS             3850..>4577
                     /locus_tag="AC16CM_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140419.1"
                     /translation="MSDTPIRVAYPAGDDLHLRIALGACQVTAGPGEGDEWIAGTYHD
                     PTGRRQLRIVEEEAGATITEEQPSFGQIPAVFGGAPRYELEFGKARPFALTIETGASD
                     FDLELGGVPLSRLVVRQGAGKFELDFARPNPARMELLDFSTGAAAIELENLANANFSE
                     LRLSGGAAGYELDFGGTLLHDAQASIETGVSAVKVTVPASTAARVVAETTLGSVDVGD
                     GYTKKEGAFLTAPALHEHSPALDIR"
BASE COUNT          754 a         1574 c         1485 g          764 t
ORIGIN      
        1 gaatctatca ggtcgagctc accactctgc aggttctggt actggacgtt ctcgtcgtct
       61 atcccgttgt agaccacctt gttcagcttt gggagcccat cctgccagta gtcagggttc
      121 ttctcgaggg tgatgtgatc ccccctcacc cgctcgacga actggaatgg ccccgtgcca
      181 acagggttct tggagattct gccgtcgctc ttctctatag cctctggcga ggccatgatg
      241 ccggccctgt cggtcagaac agcaaggaac ggcgcgaacg gctcgctgag ctcgacccgc
      301 accgtgtgat cgtccacgac ttcgaccgac tggacgggct cgatctccgt gctgcgcacg
      361 gagtccgctt tctggtaacg ctcgaggttg aacttgacgg cctcggcatc gaacggggtc
      421 ccgtcgtgga actttacgcc cttctcgagc tcgaacgtgt acgtcctgcc atctccggat
      481 acattccagc tcttcgcgag catgggcacg atcttgccgt tctcgtcgag gtccacgagc
      541 ctgtcgtaga tgctggccat aacctgcctg tcgacgtagg cgctggaaag gctggggtcg
      601 agctctggcg gctcggcctc gagcgcgacc tcgagcgtgc ctggaggcgt gctcgactcc
      661 ggggctcccc caccaccgcc gcagccggcc aaggtgagta gggccacaaa caaagacacc
      721 acaacacaaa tgcgagtacg cccgatcatc ccaccgatcc cccccgtctg cggcaaattc
      781 cgacccctct agtcaccttt gcggtgcggc aaaattagca gcaagccccg cccgagtcaa
      841 gccaccccta gcaccacaaa ggctagcccc atatcctcac cccggcttcg gcccgcaggc
      901 caacgatgca acagttcctc ggcccgatac tgttccggcc ggagctagcg ccccacggcg
      961 taaccctgca tgcccctcgg gttggccgcc cctcgcatgg acccgtccct ggcatccctg
     1021 cttacggcgg agatcctgcc gagggaccaa ccgtctacca cgtccacatc gtggtctctg
     1081 gcgcgcagac cggccacgac gtctggccct attctgtcct cgaccatgat cctgcctggc
     1141 gccgccgccc gcgggtaaaa ggagctcggg aagtgttcgg tgtggaagtt gggggcgtct
     1201 atggcctcct gcaggttgag gccgaagtgg gcatgagcca ggaagaagtg gagcgaccac
     1261 tggtcctgct ggtcgccgcc gggtgtaccg aaggccaggt agggctcccc gtcgcgcaac
     1321 gccagcgaag gcgtgagggt ggtgcgcggc ctcttgccgc cttcgagcga gttgggtagg
     1381 ccggcggtga gccagaacat ctgcgcccgc gtgccgagcg ggaagccgag gccgggcacg
     1441 acgggcgagc tctgcaacca cccgccgctc ggggtggcag agaccatgtt cccgtgccgg
     1501 tccaccacat ctcgatggca ggtatccccg ctaccctggt cgtcgcgcgg taccgtgggc
     1561 tcgccggctc cggcggctcc gtcctgtgag atgccggacc ggtcgggaag gcggggagca
     1621 cgcccgccgg ccgtaccggg gcgcagatcg taggaggcgt cctcccccac cagcatcctg
     1681 cgggacgccg tgtaggaagg atggagcaac ggttcgtgca gggcgtcgac gaaccgcggg
     1741 tcgccgtagt gggcctccct gtcagcgaag gcgagcttgg cgcactcgac gacggtgtgt
     1801 acgtactccg cggtgttgtg gcccatgctc gttagatcga acccttcgag cagcgcgagc
     1861 tgctgaagga agacgggccc ctggccccag ggacccgtct tgcagaccgt gtacccgtgg
     1921 tagtcaagcg tcgccggctc ctccagatcg gcggcgtagg cggcgaggtc gtcgcccttc
     1981 agaaggcccg tgtggcgtcg gcccgaggtg tccatcaccg tctccgagga cgcgtagcgg
     2041 tcgatggcct cggcaacgaa gcccctgtag aagacgtcgc gggcggcctc tatctgcccg
     2101 tcgcggttgc tggtcgtcgc ttcagcttcc tccaggatgc ggcggtaggt ggcggccagg
     2161 tcgggattgg agaacagggt gcccggcctc gggacacccg acccacgcag gtacacccgc
     2221 gccgaggagg gccattcatc gcggaacagg ccctccacac cgcttatagt ggcgctgatc
     2281 tggggcacga gcgggtagcc gcctccggcg tagtcgatcg cgtagcgcat cacctcggag
     2341 aggcggagcg tgccgaactc gccgagcatc agcatccagg cgtcgaaggc gccgggcacg
     2401 caggcggcga gcagcccggt accgggtacg aggtcgaggc cgagccgctc gaagtggtcg
     2461 atgctcaccg ctgccggcgc cggaccctgg cccgcgacga cgagcgtccg gcccccctcg
     2521 gcgctgtgga gcaggatcgg gacctccccc ccgggaccgt tgaggtgggg ctcgaccacc
     2581 tggagggtca ggccggccgc cacggcagca tcgaacgcgt tgccccccga ctctagcacg
     2641 gccatgcctg cggatgaggc cagccagtgc gtggacgcta ccatgccgta ggtgccgacg
     2701 agctcgggcc tggtggtaaa caagatccct atcctttctt catgtgctcg aacacagccc
     2761 ggtccaggta gccacgccgc tgctcgcgct cctcgaagtt ctcggtgcgt acgtaagctc
     2821 tgagcgcgcc gaagaggtca tcgtcacccg ccggcggccc gtccagatac ccggcgcgca
     2881 gcagcgcgcc ggcgacctgt gcccgaacgt ctccgtcaac cgcgatgacg tcatcggggt
     2941 tggtctcacc gaaatacagg gtgtgtagtt cccggatacg gatcagctca cttatgggct
     3001 cggggtgatc gtccacgcgc aggtccaaga ccctgtcgtt gtccccgccg tagcctcccc
     3061 cctcccgcac gacgagcaac gccgccgact gcttgccgcg cgagtcgcca ccggcccgct
     3121 gcccggcgtc cagcgccccg aggaggcgcg cggcgagatc gccgtcagcc tcctcataag
     3181 cacccgccat cgcctcgacg gtctctatgc cgacgaggat gttgcactgg gccgcgtagt
     3241 gttcgccggt aacgcccccc gcccagtcga aacactcgga gccggtatac gtggacgccc
     3301 ggcccctggc gtccacgacc ccgacctgcc tgtgctcgcg ctcttcgtca gccgtggtga
     3361 gggcttccac cgtctgtacc gccgaattgc ctgccgccat caggtccagg ccacgcggac
     3421 cgtagttgta gttcgccatc gcctgggtcg ccacggcccc gaccccagcg cgtgcccagg
     3481 gcaccaccga gcccacggcc aggaatttcg actgcacggc gacgccgagc gagtcggtct
     3541 cgggatcaaa ggccacgatg gagaaagtag caactatctg gtctgttctc ataccgctcc
     3601 ccgccctcct gctaggtctt cgctcatctt aacccggcat cgaaccggag acaggccggg
     3661 gagagccctc gaccgatcgc tcaccgctca ttgttagggg ggccggctgg cctcccttcg
     3721 agatgatccg cagcacctgg gcattctttc gttgccgctc gcgcgggttc aggtgttaac
     3781 cttgggtaga tgccggacgc ggctccgggg attccgccgc gttcgcgagg cacggaaagg
     3841 aggactcgca tgtcggatac gccgatacgc gttgcctacc ccgcggggga cgatctacac
     3901 ctgcggatag ccctcggagc ctgccaggtc acggcgggtc cgggcgaagg cgacgaatgg
     3961 atcgccggga cctatcacga ccccaccggc agaagacagc tcaggatcgt cgaggaagag
     4021 gcgggggcca cgatcaccga ggagcagccc tccttcggac agatcccggc cgtcttcggg
     4081 ggtgcgcccc gctacgagct cgagttcggc aaagcgagac cgttcgccct gacgatcgag
     4141 accggcgcca gcgacttcga cctcgagctc gggggcgtac ccctgagccg gctcgtggtc
     4201 aggcagggtg ccggcaagtt cgagctggat ttcgcgcgac ccaaccccgc aaggatggag
     4261 ctgctggact tctccaccgg cgccgccgcg atagagctgg agaacctggc caacgccaat
     4321 ttctccgagc tgcgcctctc cggcggggca gccggctatg aactcgactt cggcggaacc
     4381 ctcttacacg atgctcaggc gagcatagag acgggggtct cggcggtgaa ggtgactgtg
     4441 ccggcttcca cagcggcgag ggtggtcgcg gagaccacgc tggggagcgt ggacgtgggg
     4501 gatggttaca ccaagaagga gggcgccttc ctcaccgccc ctgccctgca cgagcacagc
     4561 cccgccctgg atattcg
//
LOCUS       DBNGVD010000412        10133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4469077, whole genome shotgun sequence.
ACCESSION   DBNGVD010000412 DBNGVD010000000
VERSION     DBNGVD010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4469077"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16CM_11090"
     CDS             <1..672
                     /locus_tag="AC16CM_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3140420.1"
                     /translation="SPYMTYLKLGGLSLVGASPEPLVRVEGRRVMTRPVAGTRGRGAD
                     PLEDAALAEDLLADEKERAEHVMLVDLGRNDLGRVSDVGSVTVESFMEIERYSHVMHI
                     VSTVEGDLRQDLTALDALAAAFPAGTVSGAPKVRAMEIIDELESTRRGPYAGATGYYG
                     IDGRLDTCITLRTALLKDGMAYFQAGGGVVADSVPSLEYEETRNKARAMERALEVART
                     REMWL"
     gene            complement(813..1688)
                     /locus_tag="AC16CM_11095"
     CDS             complement(813..1688)
                     /locus_tag="AC16CM_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140421.1"
                     /translation="MKDGTILRPMTAADADAVYQTSSQALPATDEERRQVMNRSTEEV
                     ERRKRRYRHFLEHDPEGAWVAVDDDRVAGIALALEREGIWVLSLLAVAEEYRDRGLGK
                     MLLDRALIYGEGCRGAMIAACTHPAAMRRYALAGFALQPTLMASGGVSRESLPAGLKA
                     REGSEEDLEVAAKIDRTIRGAPHGPDLDLLLRNCSRLLVADGPEGSGYAVLLEGSPAL
                     LAATSPGTATDLLWSCLAKSDGNEVEVHWITAAQNWALPVVLGAGLSLSPAGPLCVRG
                     ELGPLTPYLPSGPYL"
     gene            1787..2389
                     /gene="plsY"
                     /locus_tag="AC16CM_11100"
     CDS             1787..2389
                     /gene="plsY"
                     /locus_tag="AC16CM_11100"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011933831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3140422.1"
                     /translation="MLGIFLVLLGYVLGSVPTGYLVGRALGVDVRTVGSGNIGTANVL
                     RAAGKRAAVLTLAGDMVKGLIPVVVARLLTENSWLIAVVALAAVVGHCWPVFLRFKGG
                     KAVATGAGTTIGLAPLVGLGLFAFWWAVVLLSRYTSLGAISVMIVSPFVFWLTGQPLP
                     YILYTIVGGALVLWRHRENARALIKGTERKVGQRTEERGS"
     gene            2390..4645
                     /locus_tag="AC16CM_11105"
     CDS             2390..4645
                     /locus_tag="AC16CM_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HIR3140423.1"
                     /translation="MLREAYTDKMILLTGGTGFLGTALVEKILRSLPDLGRLYLLVRP
                     SRGKSASERFRKDMLGASAFRGLREELGDDFEAYVSGKVRVLEGDVHEPSLGLGGADL
                     AELSREVDVVIHSAASVIFDAPLDAAVDSNVRGTLGLLRLARGWEKRPLFMHISTAYV
                     AGTIKEDAPEAPPGNSSPNGTYLDARTEVLGLEAVVEEVDAASQESSLLRRFETEARQ
                     ELGMVGEEDEVAERVDQLRRAWMRERLVERGTLRARELGWNDVYTFTKSLAERMAVEE
                     RGEAPLVLLRPAIIESSHREPYPGWIQGTRMADPIIMAFAKGVLREFPGNPKSYVDIV
                     PVDHVVNAILAAGVRRPERPEVFQVASGERNPIRYRDLYDIVRSYFVENPLRDSGGRP
                     IQVPEWSFPGRKRVEGQLKTELTVLKVAGTLVARMPEGHMAQDARQRIARAEKRARMS
                     LYYSRIYGAYANMTSTFSTFRTAALYEAMPEWDREDFPFDITQVDWRTWMQGAHLPAI
                     TTRPGRKKRKKAAAEKPGEVAAIFDVDGTLVGSNVVSYFAWLRMQELPAPLRPLWLAG
                     FLTKIPYYWGLDKISRAHFNRAFYKNYAGWKPSRARHLGQESFAGFTLERIFPDALQQ
                     LLDHKAAGHRIVLLSGALDFLLAPMKDLADDVLCSTLAQEDGHYTGELVGAPVAGDAR
                     ARMLASFARRRNLDLSRSYAYADSISDLPMLEAVGNAVAVNPDRRLGAAAKERGWRIE
                     HWDKNGLAKKV"
     gene            4647..5882
                     /locus_tag="AC16CM_11110"
     CDS             4647..5882
                     /locus_tag="AC16CM_11110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3140424.1"
                     /translation="MRALVYRKSIPRYLLMRAAAKRIKTLETGRLSPLQLEEIEDPKL
                     PTPEWVRVRPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEIFGEVAEDNSGFLA
                     GERVVVEPALGCRVRGIAPPCPYCASGRHALCLNVARGDIGPGIQTGFCHDTGGGWSG
                     GTLVVHPSQLHRVPDPVPDEAAVAIEPLACAVHAALGSAPGPDDTSLVIGAGSVGLFT
                     VAALRHLTPAGRIICVAKHDRQRKEALRLGANEIVDPKDAYTTLPEMLGTEAYRPELG
                     KPVVMGGADRVFECVGSQRTIEDALRLTKPGGEVSLVGMPSAKSSLDLTALWYKEIGL
                     KGTYAYGAEEHEGEATSSFALALRFAPEIKLETMVGPRFRLEEYDRAIAAARSAGRDG
                     NVKVVFDLRAVSRQPSAVS"
     gene            6016..7593
                     /gene="larAH"
                     /locus_tag="AC16CM_11115"
     CDS             6016..7593
                     /gene="larAH"
                     /locus_tag="AC16CM_11115"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPN-dependent hydroxyacid isomerase,
                     LarAH14/15/16 family"
                     /protein_id="HIR3140425.1"
                     /translation="MSKPGFVTLLEKDSPPMMFNAGDAFHFEKLPEGTRVIYPPGPKD
                     PLPDPGVAIERALLEPLDMEPLHELFFPGMKLTIAFDDLSLPLPPMQRPDARQLVIEK
                     VLEKAHAKGVEDIHLIAALGLHRRMTEAELRHCLGDKIMSGYYPDRLYNYDAEDEENN
                     VTIGETEEGEEVTINRRVAEGDLLVYVNVNFIPMDGGHKSVHTGLSPYSSIRHHHTPD
                     TLSHTRSLMDPPNSAMHASFNRMGKLLDEHVKVFHIETTLNNAAFPAFANFMAKPEED
                     WGPVTKANFLAMHNATQKAPRRISRGIFHTIKGPHRLTGVYAGATEPVHARTLEDNYE
                     QQVVPVDGQADVLLLGIPYLGPYNVNSTMNPLLVYNMLLGYLFNLYRGKPLVREGGVL
                     IGTHPMPEEFNKLHHPSYIDLWNEAFPETHDIREIHERYEKRYAYDPWYRTLYRNSYA
                     YHGAHPFTVLYWAAHALDHVGNVILVGGNPETTRRMGLGRADTVAEALQIAQDTVGPN
                     PSTTYMHIPPLFMCEVS"
     gene            8080..9297
                     /locus_tag="AC16CM_11120"
     CDS             8080..9297
                     /locus_tag="AC16CM_11120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140426.1"
                     /translation="MKYLRTDGRMSFVVLLASQLAATMGFTFVLPFMPLYMHVLGVED
                     AGSAAAWAGVINSSTAVTMALAAPLWGRLGDRVGLKPMLLRATIAGSIVIGLMGLVTS
                     PWQLLALKTLQGCLTGTVAAATVLVSATAPAERAGSRLGTLQMVIFVAAAAGPFFGGA
                     FADLVGIRASFGVTAVLLASSAVLISVWVREDRSSEEAEEAGPIGADGPLPYRRLAPS
                     LLALFVIQLTIMSAAPALPGLLSTLMDEPVRVATLAGWVIATGALAASLGSVVGGKLA
                     ARYGARKVIIGTLLLAGLSALPQAEVDSVAMLWSLRLLTGLFVGTAVPVANLAIRETV
                     HPGRQGEAFGVAATATSTGNAVGPLAGGLLAASFGFGAPFLAPGVCLLAVSAIIWAAP
                     LLVVGYRTALRGH"
     gene            complement(9308..10009)
                     /locus_tag="AC16CM_11125"
     CDS             complement(9308..10009)
                     /locus_tag="AC16CM_11125"
                     /inference="COORDINATES: protein motif:HMM:NF047206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual OB domain-containing protein"
                     /protein_id="HIR3140427.1"
                     /translation="MRILVDHLTRMQPGYFCAAGIDLETGEHVRPVLRRGRLTTDLLS
                     TNKGPFDIGSVLDLGTTTDVGRAPELEDRRFDPADVRWLRDEEPDEYWNALERASRES
                     LEDIFGPALELWDESGTVDVGEGRASLGLLRPEKQPWLYVDHRGTVRLVLDYLLPSVD
                     LSVTDLRLYERDGRTPRRDLVKSLQERLEAGVEVILGVGLTRPWQKRNDDAERHWLQL
                     NNVHLRDAPLWQLEA"
     gene            complement(10015..>10133)
                     /locus_tag="AC16CM_11130"
     CDS             complement(10015..>10133)
                     /locus_tag="AC16CM_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HIR3140428.1"
                     /translation="FDVPTVLLCSEARPEHCHRRLVLEYLNREWGGIEAVHL"
BASE COUNT         1702 a         3375 c         3259 g         1797 t
ORIGIN      
        1 tcgccctaca tgacctacct gaagctggga ggtctctccc tggtcggggc ctcacctgaa
       61 cccctggtcc gggtcgaggg gcgccgggtc atgaccaggc ccgtcgccgg tacccgcggc
      121 cgcggcgccg acccgttgga agatgccgcc ctggccgagg acctcctggc cgacgagaag
      181 gagcgggccg agcacgtcat gctcgtggac ctcggccgca acgaccttgg gcgcgtgagc
      241 gatgtcggtt ctgtaacggt cgagagcttt atggagatag agcgctactc gcacgttatg
      301 cacatcgtct ccaccgtcga aggagacctg cgtcaggatc tgaccgccct ggacgccctc
      361 gccgccgcct tccctgccgg cacggtttcg ggggccccca aggtcagggc catggagatc
      421 atcgacgagc tcgagagcac ccgccgcggc ccctacgccg gcgccacggg ctactacggt
      481 atagacggcc gcctggacac ctgcataacg ctccgcacag ccctgctcaa ggacggcatg
      541 gcctacttcc aggctggagg cggcgtcgtg gccgactccg taccatccct cgaatacgaa
      601 gagacccgca acaaagcaag ggccatggaa cgcgccctcg aggtcgcccg cacccgcgag
      661 atgtggcttt agcggcaagc cgcaggcttg ccgcgctgtc agctatcagc tttttcgtgc
      721 ggccccatcc gtgtcgcgct caccaaccct gtgtccttgc ggagatgggt tgtcacaata
      781 ggatcctcca ccttcagctg ccagctctta ctctacaaat acggtccgct cggcaggtag
      841 ggtgttagtg ggcccagttc gcctcgtacg cagagtgggc ctgcgggcga gagggagagg
      901 ccggctccaa gcaccaccgg gagcgcccag ttctgggcag ccgtgatcca gtgcacttcg
      961 acctcgttgc catcgctctt ggcgaggcag gaccagagca ggtcggtggc cgtcccgggc
     1021 gaagtggcgg cgaggagggc cggggagccc tctagtaaga ccgcgtatcc gctgccttcg
     1081 ggaccgtcgg caacaagcaa tctcgagcag ttccggagca acaaatccag gtctggcccg
     1141 tgtggggcgc cccgtatggt cctgtctatc ttggcggcta cctccaggtc ctcttctgac
     1201 ccttccctgg ccttcagccc cgccggcagc gattcacggc ttaccccgcc gcttgccatg
     1261 agggttggct ggagcgcgaa gcctgccagg gcgtagcggc gcatggccgc cgggtgtgtg
     1321 caggcggcga tcatggcccc ccggcatcct tctccgtaga tgagggcgcg gtcgagcaac
     1381 atctttccca ggccccgatc cctgtactcc tcggcgacgg cgaggaggga gagaacccat
     1441 atgccctccc tctctagcgc cagtgcgatg ccggctaccc tgtcgtcgtc aacggcgacc
     1501 caggcgcctt ccggatcgtg ctcgaggaag tgcctgtagc gcctcttgcg ccgttcgacc
     1561 tcctctgtac tgcggttcat tacctgccgg cgctcttcgt cggttgccgg cagggcctgg
     1621 ctgctcgtct ggtagacggc gtcggcgtcg gcggccgtca tggggcgcaa tatggtgccg
     1681 tctttcatgg cggcatgtta acccacctcg tctcgtagag atgggaagcg ttcggctttc
     1741 gcaggcggcg gcccaggatt cgtcggtccg ctatactctt tgcgccatgt tggggatatt
     1801 tctggtgctg ctcggctacg tgttgggctc tgtgccgacc gggtacctgg tcgggcgggc
     1861 cctcggcgtg gacgtgcgga cggtgggcag cggcaacatc ggcaccgcca acgtgctcag
     1921 ggccgctggc aaacgggccg cagtgctcac gctcgcgggc gacatggtaa agggtcttat
     1981 ccccgtggtg gtcgccaggc tcctcacgga gaactcctgg ctcatcgcgg tggttgccct
     2041 ggccgcggtg gttggccact gctggccggt cttcctgcgt ttcaaggggg ggaaggccgt
     2101 ggcaacgggg gcaggaacga ccatcgggct tgccccgctc gttggcctag ggctcttcgc
     2161 cttctggtgg gcggtcgtgc tgctgagccg ctacacgtcg cttggcgcca tctcggtcat
     2221 gatcgtcagc cccttcgtgt tctggctgac cggtcaaccc ctcccttata tcctgtacac
     2281 catcgtgggc ggggcactcg tcctctggcg gcacagggag aacgcgcggg ccctcatcaa
     2341 gggcaccgag aggaaagtag gacagcgtac cgaggagagg gggagctagt tgctccgcga
     2401 ggcgtacacg gacaagatga tactgctgac cggcgggacc ggctttctag ggacggcgct
     2461 ggtggagaag atcctgcgtt ccctcccgga tctcggtcgc ctttatctgt tggtcagacc
     2521 ttccaggggg aagagcgcct cggagcggtt ccggaaggac atgctcggcg cctcggcctt
     2581 ccgcggcctg cgcgaggagc tgggcgatga tttcgaggcc tacgtctccg ggaaggtacg
     2641 cgtgctggag ggtgacgttc acgaaccctc gctcgggctg gggggtgcgg acctcgccga
     2701 gctctcccgc gaggtggacg tggtgatcca ctccgcggct tcggtgatct tcgacgcccc
     2761 cctcgacgcc gcggtcgact cgaacgtccg cggtaccctc gggcttctga ggctcgcgcg
     2821 cggctgggag aagaggccgc tgttcatgca catctccacc gcctacgtgg ccggaaccat
     2881 aaaagaggac gcgcccgagg cgccgccggg caacagctcg cccaacggca cgtacctgga
     2941 cgcccgtacc gaggtgctcg gcctcgaggc cgtggtagag gaggttgacg cggcctcgca
     3001 ggaaagctcc ctgctcaggc gcttcgagac ggaggcgcgg caggagctcg ggatggtcgg
     3061 ggaggaggac gaggtcgcgg agagggtgga ccagctcagg cgtgcctgga tgcgcgagag
     3121 gctcgtcgag cgtgggacct tgcgggctcg ggagctcggc tggaacgacg tctacacctt
     3181 caccaagtcg ctcgccgagc ggatggccgt agaggagagg ggtgaggcgc cgctcgtcct
     3241 actcaggccc gccataatag agagcagcca cagggaaccc tatcccggtt ggatccaggg
     3301 cacaaggatg gccgacccca taatcatggc cttcgccaag ggcgtgttgc gcgagtttcc
     3361 gggcaacccg aagagctacg tggacatcgt gccggtagac cacgtggtga acgccatcct
     3421 ggccgccggc gtcaggcgtc ccgagaggcc cgaggtcttc caggtcgcct cgggggaacg
     3481 taacccaatc cgctaccgtg acctctacga catcgtgcgc tcctacttcg tggagaaccc
     3541 tctgcgggat tctggcggac gccccatcca ggttcccgag tggtcctttc cgggacgcaa
     3601 gcgggtcgag gggcagctca agaccgagct aaccgtcctg aaggtggcgg gcacgctcgt
     3661 ggcgaggatg ccggaggggc acatggccca ggatgccagg cagaggatag cccgcgccga
     3721 gaagcgggcc aggatgagcc tgtactacag ccgcatctac ggcgcctacg cgaacatgac
     3781 gtccaccttc tcgaccttcc ggaccgcggc tctctacgag gccatgcccg aatgggaccg
     3841 cgaggacttc ccgttcgaca tcacccaggt cgactggcgg acctggatgc agggcgccca
     3901 cctgcccgcc atcaccaccc gcccgggccg caagaagcgc aagaaggccg ccgccgagaa
     3961 gccgggggag gtggccgcca tcttcgacgt ggacggaacg cttgtcggct ccaacgtggt
     4021 ctcgtacttc gcctggctca ggatgcagga gttgcccgcg ccgttgcgcc cgctctggct
     4081 cgccggtttt ctgaccaaga tcccctacta ctggggcctg gacaagatca gccgcgccca
     4141 cttcaaccgc gccttctaca agaactacgc cggctggaag ccctcacgcg cccgtcacct
     4201 cggccaggag agctttgccg gctttaccct ggagcgcatc tttccggatg cattgcagca
     4261 gcttcttgac cacaaggccg cgggccaccg catcgtgctg ctatcaggcg ccctggactt
     4321 cctcctcgcg cccatgaagg acctcgccga cgacgtgctc tgctctaccc ttgcccagga
     4381 ggacggccac tacaccgggg agctggtcgg tgcccccgtc gccggggatg ccagggccag
     4441 gatgctcgcc tcgttcgcca ggcgccgcaa cctcgacctc tctcgctcct acgcctacgc
     4501 tgattcgatc tccgacctcc ccatgctgga ggccgttgga aacgccgtcg ccgtcaaccc
     4561 ggaccgccgt ctcggggccg cggcgaagga gaggggctgg aggatagaac actgggacaa
     4621 gaacggcttg gccaagaagg tctagcgtgc gggccctcgt ctacaggaag tccatccccc
     4681 gctacctcct gatgcgtgcc gccgccaaac gtatcaagac ccttgagacg ggccgcctct
     4741 ccccgctcca gctcgaagag atagaggacc ccaaacttcc gacgccggaa tgggtgcgcg
     4801 tgaggccgct gctctccggc atctgcggct ccgacctggg cacgctgtcc tccgagaact
     4861 cgccctactt ctccccgatc acctccccac ccttcgtgat gggccacgaa atattcgggg
     4921 aggtggcgga ggacaactcg ggcttcctgg caggagaacg cgtggtggtg gaaccggccc
     4981 tcggctgcag ggtgcgcggc atcgcccccc cctgtcccta ctgcgcctcg ggccgccacg
     5041 ccctgtgcct gaacgtggcc aggggcgata taggcccagg catacagacg ggcttctgcc
     5101 acgacaccgg cggtggctgg tcgggtggga cgctcgtcgt ccacccctcg caactccacc
     5161 gcgttcccga ccccgtgccc gacgaggcgg ccgtcgcaat cgaaccactc gcgtgcgcgg
     5221 tgcacgccgc tctcgggtcg gcccccggcc cggacgacac cagcctcgtg ataggcgccg
     5281 gcagcgtcgg cctcttcacc gtcgcagccc tccgccacct cacgccggcc ggcaggatca
     5341 tctgcgtcgc caagcacgac cgccagcgaa aagaggcgct ccgtctcggg gctaacgaga
     5401 tcgtcgatcc gaaggatgcc tacaccacct tgcccgaaat gctaggtacg gaagcctacc
     5461 ggcctgagtt gggcaagccg gtcgtcatgg gtggggccga cagggtcttc gaatgcgtcg
     5521 gttcccagcg aaccatcgag gatgccctcc gcctcacgaa gcccggaggc gaggtctctc
     5581 tcgtcgggat gccttcggcg aagagcagcc tcgacctgac cgcgctctgg tacaaggaga
     5641 tcgggctcaa gggtacctac gcctatggcg ccgaagaaca cgagggagag gcgacgagca
     5701 gcttcgccct cgccctccga tttgcgcctg agataaagct cgaaacgatg gtaggaccac
     5761 gtttcaggct ggaggaatac gacagagcca tcgccgctgc caggtcggcg ggccgcgatg
     5821 gcaacgtgaa ggtggtcttc gatctcagag cagttagccg ccagccatca gcggtcagct
     5881 agaatcacta cggagttggt gctggtatga aaacgaacct gtgctgccag agccgagcgt
     5941 gtgggctgag cagcaggtag gacgggcgca cgaaaagctg aaagctgaca gctagagcga
     6001 ccgaagggag cgacattgag caagccgggt ttcgtaacgc ttctggagaa ggacagcccg
     6061 ccgatgatgt tcaacgcggg ggacgccttt cacttcgaga agctaccaga ggggacgcgg
     6121 gtgatctacc cacctggtcc gaaggatccc ctgcccgacc ccggcgttgc gatcgaacgt
     6181 gcgctcctgg aaccgcttga catggagccg ctccacgagc tgttcttccc cgggatgaag
     6241 ctcaccatag ccttcgacga cctctcgttg ccgctcccgc ccatgcagcg gcccgacgcg
     6301 aggcagctgg tgatcgagaa ggtgctggag aaggcccacg ccaagggcgt cgaggacata
     6361 cacctcatag cggccctcgg cctgcaccgc aggatgaccg aggccgagct gaggcattgc
     6421 ctgggcgaca agataatgtc gggctactat cccgaccgcc tctacaacta cgacgccgag
     6481 gacgaggaga acaacgtcac catcggcgag acggaagagg gcgaggaggt gacgatcaac
     6541 cgccgcgtgg ccgaaggcga cctgctggtc tacgtgaacg tcaacttcat ccccatggac
     6601 ggtgggcaca aatccgtcca cacgggcctc tccccgtact cctcgatccg ccaccaccac
     6661 accccggaca cgctctcaca cacccgctcc ctgatggatc cccccaactc ggccatgcac
     6721 gcctccttca accgcatggg caagctgctc gacgagcacg tcaaggtgtt ccacatcgag
     6781 accaccctaa acaacgccgc cttccccgct ttcgcgaact ttatggccaa accagaggag
     6841 gactggggac ctgttacgaa ggcgaacttc ctggccatgc acaacgccac ccagaaggcg
     6901 ccgaggcgca tatcgcgcgg gatcttccac accatcaagg gcccgcacag gctgacgggc
     6961 gtgtacgccg gggccacgga gcccgttcac gccaggacgc tagaggacaa ctacgagcag
     7021 caggtggtgc cagtcgacgg ccaggccgac gtgctgctcc tcggcatccc gtacctcggg
     7081 ccctacaacg tcaactccac catgaacccg ctcctcgtct acaacatgct gctcggctac
     7141 ctcttcaacc tctacagggg aaagccgctc gtgcgcgagg ggggtgtcct gatcggcacc
     7201 caccccatgc ccgaagaatt caacaaactc caccacccga gctatatcga cctctggaac
     7261 gaagctttcc ccgaaacgca cgatatacgc gagatccacg agcgctacga gaagcgctac
     7321 gcctacgacc catggtaccg gaccctctac cgcaactcct acgcctacca cggtgcccat
     7381 cccttcaccg ttctctactg ggcagcccac gccctcgacc acgttggtaa cgtgatcctg
     7441 gtcggaggca accccgagac aaccaggcgt atgggcctgg gccgcgccga cacggtggcc
     7501 gaagccctcc agatcgccca ggacacggta ggccccaacc cgagcaccac ctacatgcac
     7561 atcccgcccc tctttatgtg cgaggtgtct tagagcggtc agctaatttg gagtggtcag
     7621 ctgatagcct tcagctttca gctttttgac cgggtgtgtt ttgcgtgggg gctctagttt
     7681 tgcctggcgc ccttttgggc tcgtaggctc acgcgccgcg cagcgcgtga gccttttcac
     7741 ttcgggggcc tgtgtggagg ctacaaagtc tgatttgctg cggtgttctt gatacactct
     7801 gtagcgaaag actttttgag aatcgtatcc ggagtgagcg ttttcgagcc tgtacgggag
     7861 ggtgtcccgc gcctccgtgg cgtggtttgc ctgtcctgtt tatgtggctt tcaggccgct
     7921 tgtctagtgc ggtgtttgcc gctgctcacg agccccggag catccgggaa ggagagttac
     7981 gctacagagg gttgcagggt tgctgttcag ggcggccgcg catagaagca tattcgacat
     8041 aaaggtctgc gccttccgcc gcttcgcgga gggtgacagg tgaagtattt gagaacagac
     8101 gggagaatgt ctttcgtcgt tctgctcgcc tcgcagctcg ctgcgaccat ggggttcacg
     8161 ttcgttctgc cgttcatgcc gctctacatg cacgtgctcg gagtggaaga tgcgggcagc
     8221 gcggcggcgt gggctggcgt gatcaacagc tccacagccg tgaccatggc cctcgctgcc
     8281 ccgctgtggg gcagattggg cgaccgcgtg ggtctcaagc ccatgctctt gagggccacc
     8341 atcgccggtt ccatcgtcat cggcctcatg ggcctcgtga cgagcccgtg gcagttgctg
     8401 gccctcaaga ccctgcaagg ttgcctgaca ggcacggtcg ccgccgcgac cgtcctagtg
     8461 tcggcgacgg ccccggccga gagggccggc tcgaggctcg gaaccttgca gatggtcatc
     8521 ttcgtggccg cagcggccgg gcccttcttc gggggcgcct tcgccgacct ggtcggcatc
     8581 agggcctcct tcggcgtaac ggcggtgctc ctggcgtcgt cggcggtcct gatctccgtc
     8641 tgggtcaggg aagacaggtc aagcgaggag gcggaggagg cggggcccat cggggctgat
     8701 ggcccgctgc cgtacaggag gcttgcgccg agcctcctcg cgctcttcgt gatccagttg
     8761 acgatcatga gcgcggcgcc ggccctgccc ggtctgctct cgaccttgat ggacgagccc
     8821 gtaagggttg ccaccctggc gggttgggtt atcgccacgg gggccctggc ggcctcgctc
     8881 ggctccgtgg ttggggggaa gctcgccgcc agatacgggg ccagaaaggt cataatcggg
     8941 acgctgctgc tggccggcct ctcggcgctt ccgcaggccg aggtcgactc cgtggccatg
     9001 ctctggtccc tgcggctcct cacagggctc ttcgtcggta ccgccgtccc ggtagccaac
     9061 ctggctatcc gggaaacggt gcaccccgga cgccagggag aagccttcgg ggtcgcagcc
     9121 acggcaacct ccacgggcaa cgccgtcggg ccgctcgccg gaggactgct tgccgcctcg
     9181 ttcggcttcg gcgcaccttt cctggcccct ggcgtctgcc tcctcgccgt ctccgcgatc
     9241 atctgggcag caccgctgct agtcgtcggc taccgcaccg cgttacgagg tcattagtag
     9301 ctatcagcta tgcttccaac tgccagagtg gggcgtctct gaggtggacg ttgttgagtt
     9361 gtagccagtg cctctcggcg tcgtcgtttc gcttctgcca gggacgcgtc aggccaaccc
     9421 cgaggatcac ctctacgccg gcttcgaggc gttcttgcag gctctttacg aggtcgcggc
     9481 gcggggtacg tccgtccctt tcatagagcc gcagatccgt gaccgacagg tctaccgagg
     9541 gcagtaggta gtccaggacc aggcggaccg tgcccctgtg gtccacgtac agccagggct
     9601 gtttttcggg ccggaggagg ccaagcgacg cacgcccctc gccgacatcg accgtgccgc
     9661 tctcgtccca cagctcgagc gctggcccga agatgtcctc caggctctcc cgagacgccc
     9721 tttcgagggc gttccagtac tcgtcgggct cctcgtcacg cagccatctc acgtctgccg
     9781 ggtcgaagcg cctgtcctcc agctctggag ctcgcccgac gtcagtcgtc gtaccgaggt
     9841 cgaggacgga cccgatatcg aaggggcctt tgttggtgct gagcagatcg gtggtcagcc
     9901 tgccgcggcg taggaccggg cggacgtgct ccccggtttc gaggtctatg ccggcggcgc
     9961 agaagtaccc cggctgcatg cgcgtcaggt ggtctacgag gatccgcatg ctacctagag
    10021 gtggacggcc tcgatgccgc cccactcgcg gttcaggtac tcgagtacca ggcgccggtg
    10081 gcagtgctcg ggtcttgcct cgctgcacag cagcaccgtc ggaacatcga aga
//
LOCUS       DBNGVD010000413         5211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3377077, whole genome shotgun sequence.
ACCESSION   DBNGVD010000413 DBNGVD010000000
VERSION     DBNGVD010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3377077"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="AC16CM_11135"
     CDS             complement(<1..495)
                     /locus_tag="AC16CM_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140429.1"
                     /translation="MGTGAITIVLIFLLLFVSWQTIYAANRDLARAKAWGDLLRRALP
                     FFALLLMVLSLFVIQSFGQGWTLLAWMAVAGAAIIGASLLSITSLERNANRAFRSGDY
                     ARAVELYEKLVQEKPLARNKAFLGAALGASERNEESVDLSTQAIEQDPEYGLAYYNRA
                     LVLQR"
     gene            complement(503..1087)
                     /locus_tag="AC16CM_11140"
     CDS             complement(503..1087)
                     /locus_tag="AC16CM_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HIR3140430.1"
                     /translation="MDSLRYALKEWAVAVKALERGETALVVRKGGIREKAFAVPKTRF
                     LLRPGYEHQRPELLKSEHRHLMDGIPDLTDDGPLRFSSFAEVSGAYEISEPEELAALD
                     PHHMWTSEYAESRFKWRPKKPLTVLVLRTYLLPETVELPFVDEYGGCKSWIELQKTVP
                     VEGARPALSEEDFQALVAPALGVLRTLEPAPVGA"
     gene            1176..2573
                     /locus_tag="AC16CM_11145"
     CDS             1176..2573
                     /locus_tag="AC16CM_11145"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3140431.1"
                     /translation="MREQETPHRVERDSMGEVEVPRDALFGAQTCRALDNFPISDLRK
                     PRRFVEAIGAIKLEAAVVNHELGLIGEQDKDAIVEAAEEVVDGRLDSAFVLDVFQTGS
                     GTSTNMNANEVISNRAIQILGGELGSKSPIHPNDHVNMGQSSNDVIPTAIHLAALVSI
                     DKDLLPALEKLLSALREKAVQFDDVIKTGRTHLQDATPIRLGQEFRGYAGQMERGIAR
                     LRKAQEDLSEVALGGTAVGTGVNTQPEFAARVCERLSSRFGVDVHETENHFQAQSAMD
                     ATVFTSGALKTVAVSLMKISNDIRWLGSGPRAGIGEIALPEVQPGSSIMPGKVNPVIA
                     ESAAMVAAQVMGNDSTITIAGQSGNFELNVMLPLIAYNLLQSIDLLSNAAENLTDQCV
                     SGIEATERGPELVEQGLMLATALAPEIGYDRAAEISKAAYKTGKTIREIAREETDLSE
                     EELDDLLDARHMTGN"
     gene            2589..3422
                     /locus_tag="AC16CM_11150"
     CDS             2589..3422
                     /locus_tag="AC16CM_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3140432.1"
                     /translation="MSGSRDNSQGINVKVFEDVDDSEASQAYIDILDKFDALPGIQVL
                     KQGAIERCRLREGMSVLDSGCGAGLETVRLARLVAPSGKVTGLDASQAFLAEARKRAG
                     GLRLPIEYRQGDAQRLPFADRSFDVARAERLLLYLQEPATAIAELARVTRKKGSVYVI
                     EPDFETVTINVGNRALVRKVLNFDCDKDTRNGWIGRELPGMFAAAGLVDVEVQTGVVV
                     FEPVAFSPYFLEIGAAAHEGGAITADELAQWQGEIRDLLKRDQLFCTITYFMIFGRVA
                     P"
     gene            complement(3419..3889)
                     /locus_tag="AC16CM_11155"
     CDS             complement(3419..3889)
                     /locus_tag="AC16CM_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140433.1"
                     /translation="MRGAITLTAAQTLPEDTPSRSLLILIAFLVATSSLLLQGGTLAP
                     LISRLQPAVPDESAIHDERVRLMALLEQTAAAVAEERGVDPARVSRGGLEWAADHAED
                     QKRLALDVISTQRRALLDARDDGDFSAEALNAVLAVLDADQISLELKGAPADLD"
     gene            complement(3896..4282)
                     /locus_tag="AC16CM_11160"
     CDS             complement(3896..4282)
                     /locus_tag="AC16CM_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140434.1"
                     /translation="MFLIMGLELSAIVSDVGMDSRSIGIAAGLAAVALLLTILVRVVY
                     VAPLLAGLKLHASRGARLKERIATIQDRFGGLDVASKAHDPFWERRMPPAERVDRFRG
                     RLRRLVADIDYLIAHALGPREGTIIV"
     gene            complement(4224..4787)
                     /locus_tag="AC16CM_11165"
     CDS             complement(4224..4787)
                     /locus_tag="AC16CM_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3140435.1"
                     /translation="MVELLIVVVMGLLVISAAATFGPRLGFAAPLLLVVVGIGVSLLP
                     FAPEVHVEPEWILSGVLPPLLYSAAVSMPSMNFRREFTAIGGLSFVLVVVSSLVVGVV
                     FAWLVPGLGLVWGIALGAIVSPTDAVATSIVKRAGAPTRVVTIPVCAAPAIRFPELAH
                     GRADPRRRRVSDHGPGALRHRQRRRYG"
     gene            5012..>5211
                     /locus_tag="AC16CM_11170"
     CDS             5012..>5211
                     /locus_tag="AC16CM_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3140436.1"
                     /translation="MALAARRGFGGQGPGGHGQAGFPFGGVRGGGFGHRPRRMGQGDV
                     RGAVLVLLSERPMHGYQIIQEIG"
BASE COUNT          998 a         1679 c         1706 g          828 t
ORIGIN      
        1 ccgttgcaag acgagggcgc ggttgtagta ggcgaggccg tactcggggt cctgctcgat
       61 ggcctgcgtg gagaggtcca cggattcttc gttacgctcg gaggccccga gggcggcccc
      121 cagaaaagcc ttgttgcggg cgaggggctt ttcctggacg agcttctcgt agagctcgac
      181 cgccctcgcg tagtcgccgc tgcggaaggc cctgtttgcg ttgcgctcca gggacgtgat
      241 ggagaggagg ctggccccga ttatggcggc gccggccacc gccatccagg ccaggagcgt
      301 ccagccctgc ccgaagctct ggatcacgaa caggctcagc accatcagta gcagggcgaa
      361 gaagggtagg gcgcggcgta gcagatcccc ccacgccttc gccctcgcca ggtcgcggtt
      421 ggcagcgtag atggtctgcc agctcacgaa gaggagaagg aagatcagga ctatggttat
      481 cgctcccgtg cccatcgcta cctcaggcgc cgaccggcgc gggttcgagg gtccgaagca
      541 ccccgagtgc gggcgcgacg agcgcctgga agtcttcctc cgagagcgct ggtctggccc
      601 cctcgacggg aacggttttc tggagctcga tccaggactt gcagcccccg tactcgtcga
      661 cgaaaggaag ctcgacggtc tcgggaagca ggtaagtgcg caagacgagg accgtcagcg
      721 gctttttggg gcgccacttg aagcggctct ccgcgtattc cgaggtccac atgtggtgcg
      781 ggtcgagggc cgcgagctcc tccggctcgg agatctcgta ggccccggag acctcggcga
      841 acgaggagaa gcgcagggga ccatcatcgg tgagatcagg gatcccatcc atcaggtggc
      901 ggtgctcgct cttcagcagc tctggtctct ggtgctcgta gccgggacgg agcaagaacc
      961 gagtcttcgg cacggcgaag gccttctccc tgatgccgcc cttcctgacc accagcgcgg
     1021 tctcgccgcg ttcgagggcc ttcacggcga ccgcccactc cttgagcgcg tagcgtagag
     1081 aatccaagac atcacctctc gtataatgcg accgttgcac gagtgattat atgactatct
     1141 gggacggacg tttccgcccg tgaaggaggc ctcacgtgag agagcaagag acgccgcacc
     1201 gcgtagagcg ggactccatg ggggaggtcg aggtaccccg cgacgccctc ttcggcgcgc
     1261 agacctgccg cgccctggac aacttcccga taagcgatct gcgcaagccg cgccgcttcg
     1321 tcgaggctat cggcgccata aagctggagg cagccgtcgt caaccacgag ctgggcctga
     1381 tcggcgaaca ggacaaggac gccatcgtgg aggcggccga agaggtcgtc gacggaaggc
     1441 ttgacagcgc cttcgtcctc gacgtatttc agaccgggtc gggcacctcg accaacatga
     1501 acgccaacga ggtgatctcc aaccgcgcca tccagattct cggcggggag cttggctcga
     1561 agagccccat ccaccccaac gaccacgtca acatgggcca gtcctcgaac gacgtgatcc
     1621 caacggcaat ccacctggcg gcccttgttt ccatcgacaa ggacctcctg cctgccctgg
     1681 aaaagcttct gagtgccctg cgggagaagg ccgtccagtt cgacgatgtg atcaagacgg
     1741 gccgcacgca cctgcaggac gctaccccga tccggctcgg ccaggagttc cgggggtacg
     1801 ccggtcagat ggagcgcggc atcgcgaggc tgcgcaaggc gcaggaggac ctctcggagg
     1861 tggcccttgg cggcaccgcc gtcgggaccg gcgtcaacac tcagcccgag tttgccgccc
     1921 gcgtttgcga gcggttgagc tcccgcttcg gcgtcgacgt gcacgagacg gagaaccact
     1981 tccaggccca gagcgcgatg gacgccaccg tgttcaccag cggcgccctg aagaccgtcg
     2041 cggtcagcct catgaagatc tccaacgata tccggtggct cgggagcggg ccgcgggccg
     2101 gcatagggga gatcgccctg cccgaggtgc agccgggctc ctccatcatg cccggcaagg
     2161 tgaaccccgt gatcgccgaa tcggccgcca tggtcgcggc ccaagtgatg ggcaacgatt
     2221 ccaccattac tatcgccggc cagagcggaa acttcgagct gaacgtgatg ctgcccctca
     2281 tcgcctacaa ccttctccag tccatcgacc tgctctccaa cgccgccgag aacctgacag
     2341 accagtgcgt gagcggcata gaagccacgg agcgtggccc cgagctggtc gaacagggcc
     2401 tgatgctcgc cacggccctg gcacccgaaa tcggctacga cagggccgcc gagatctcca
     2461 aggcagccta caagacgggt aaaaccatcc gcgagatagc ccgcgaagag accgacctct
     2521 ccgaagaaga gctcgacgat ctactcgacg ccaggcatat gacaggaaac tgaagagggg
     2581 gttctccggt gtccggtagc agggataatt cacaggggat caacgtcaag gtcttcgagg
     2641 acgtagacga ctccgaggcc tcgcaggcgt acatcgacat actggacaag ttcgatgcgc
     2701 tgcccggcat ccaggtgttg aagcagggcg ccatcgaacg gtgcaggctc cgggagggta
     2761 tgtcggtgct ggacagcgga tgcggagcgg gcctcgagac cgtcaggctg gccaggctcg
     2821 tcgcgccgtc gggcaaggta accgggctcg acgcgagcca ggcgtttctc gcggaggcca
     2881 gaaagcgcgc cgggggcctg aggcttccca tcgagtaccg gcaaggcgat gcccagcgtc
     2941 tgcctttcgc cgaccgctcc ttcgatgtgg cccgcgccga gaggctgtta ctgtacctcc
     3001 aggaaccggc cacggcgatc gccgagctcg cgcgcgtgac caggaaaaaa ggctccgtgt
     3061 acgtcataga acccgacttc gagaccgtta ccataaacgt gggcaacagg gctctcgtcc
     3121 gcaaggtcct caacttcgac tgcgacaagg acaccaggaa cggttggatc ggccgcgagc
     3181 tacccggcat gttcgcggcc gccggcctcg tggacgtcga ggtgcagacc ggcgtggtcg
     3241 tcttcgagcc cgtagccttc agcccgtact ttctcgagat cggtgcggcg gcccacgaag
     3301 gtggcgccat caccgcggac gaactcgcgc agtggcaggg tgagatcagg gacctcctga
     3361 aacgggatca gctcttctgc acgatcacgt acttcatgat attcgggcgc gtggcaccct
     3421 aatcgaggtc cgctggcgct ccttttagtt cgaggctgat ctggtcagcg tccagtacgg
     3481 ccaggacggc gttcagcgcc tccgcgctga agtctccatc gtctctagca tcgagcaggg
     3541 ctctacgctg tgtcgagatc acgtcgaggg ccagtcgctt ctggtcctcg gcgtgatccg
     3601 ccgcccactc taaacctccg cgcgataccc gtgcaggatc gaccccgcgt tcctccgcga
     3661 ccgcggcggc ggtctgctcc agcagagcca tgagccgcac ccgttcgtcg tggatggcgg
     3721 actcgtccgg gacggccggt tggaggcgcg agataagggg cgcgagggtg ccaccctgca
     3781 gaagcaggga actggtggcg accaggaacg cgatcaggat cagcagcgac ctgctagggg
     3841 tatcctcggg gagggtctgg gcagcggtga gggttatggc gccgcgcatc ccggcctaca
     3901 cgatgatggt tccttcccgc gggccgaggg cgtgcgcgat cagatagtcg atgtccgcga
     3961 ccagacgacg cagtcgccct cgaaaccggt caaccctctc ggcggggggc attcgtctct
     4021 cccagaacgg atcgtgggcc ttcgaagcaa cgtcaagtcc gccgaaccgg tcctggatcg
     4081 tggctatcct ctctttgagc cgtgcgccac ggctggcatg aagctttagc ccggccagca
     4141 ggggtgcgac gtagaccaca cggaccagga tagtaagcaa cagggccacg gccgcgaggc
     4201 ctgccgcgat cccgatgctc ctgctatcca taccgacgtc gctgacgatg gcggagagct
     4261 ccaggcccat gatcagaaac acggcgcctt cgaggatcag ctcgaccgtg cgccagttct
     4321 gggaatcgga tagccggtgc tgcgcagaca ggaatcgtca ccacccgggt cggggccccg
     4381 gcacgcttca ctatcgaggt ggcgaccgcg tcggtcgggc tgactatggc gccgagcgcg
     4441 atcccccaca ctagaccgag gccagggacg agccaggcga agaccacccc gaccaccagc
     4501 gagctcacga ccacgagcac gaacgacaga ccaccgatgg cggtgaactc gcggcggaag
     4561 ttcatcgagg gcatcgacac cgccgccgag tacagaagcg gcggcaacac gccggacagg
     4621 atccactccg gttcgacgtg gacctcgggg gcgaaaggca gcaggctcac ccctatcccc
     4681 accaccacga ggagcaacgg cgccgcaaac ccaagccgtg gcccgaacgt ggcagcagcc
     4741 gagatcacca acagccccat gaccaccacg atcagtaact ccaccatgct ccctccgtct
     4801 cccgacccag ggacgccgcc ccagcatctg gcgacctccg cgattgtatc cgcgcaactt
     4861 gctatcggag cttgacaaac gacatactac gatatatcgt tgatataaca caaaggctgg
     4921 aagccagaga ggagcaatga tgtacggacc acaagcggta ggaggaccga tggttggagg
     4981 gctgcacgag cacctggggc gtcgcttgcg gatggccttg gccgcccgtc gtggctttgg
     5041 gggtcagggg cctgggggtc atggacaggc ggggttccct tttgggggag tccgtggggg
     5101 cgggttcggg caccgtccca ggcgaatggg gcagggggac gtgcggggcg ctgtcctcgt
     5161 cctgctctcg gagcgtccca tgcacgggta ccagatcatc caggagatcg g
//
LOCUS       DBNGVD010000414         5550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1920246, whole genome shotgun sequence.
ACCESSION   DBNGVD010000414 DBNGVD010000000
VERSION     DBNGVD010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1920246"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16CM_11175"
     CDS             <1..335
                     /locus_tag="AC16CM_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3140437.1"
                     /translation="AAKREVQDLADGYSPPERGANVYAAARSARAALELGVKTMQWGH
                     YASEYDGVIAGHVARVLTGGGLSLPQWVPEEYLLKLEKEAFLDLLQQEKTHERIEALL
                     TTGKPKRN"
     gene            427..1605
                     /locus_tag="AC16CM_11180"
     CDS             427..1605
                     /locus_tag="AC16CM_11180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3140438.1"
                     /translation="MNEAVIVSGVRTAVGRAPRGTLRASRPDDMAAAAIGEAVRRAEG
                     LAREDVEDVIIGCAMPEGTQGYNVARLSSLLAGMPDSVPAQTVNRFCASGLQTIAMAA
                     ERIMVGNAKTIVAGGTEHMSSTLEMPNFSPNSRLMEINPAAYMGMGLTAEQVAREFDV
                     NREDQDEFALRSHTLAKKAVEGGVFDEEIVPLDVEFDWVDESGEAQHAETSFGRDEGP
                     RDTSAEKLAKLRPAFQRDGTVTAGNSSQRSDGAAAVVVMDREEAERRGLTPLARFVGF
                     AVGGVRPEVMGIGPTVAIPKVFEQTGLTMDDIDLIEFNEAFAAQALAVMREVGMDLDK
                     TNVHGGAIALGHPMGATGTKLTVQLINEMKRRGSRYGLVTMCIGGGMGAAGIFENLQN
                     "
     gene            1646..2533
                     /locus_tag="AC16CM_11185"
     CDS             1646..2533
                     /locus_tag="AC16CM_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140439.1"
                     /translation="MMNVPAWNHQFVGANGIDIHYVRHGEGYPLVLLHGWPEFWYGWH
                     KNIPVLSERFDVVAPDLRGFGDTDKPGGGPPGELLGNLVEDLCGLADALGFERFGLVC
                     HDVGAYVGQGFARKYPERLGGLFFFNCPYPGIGKRWVDPESVNEIWYQSFNQQPWAAS
                     LVGENRKTCEVYFRHFLDHWAHEPGLFDDDLDAWVDNFMKPGNLQGGFDWYIGSNESR
                     LDLIRNGPPEMPRIETPTRIRWGESDPILKVEWADRLGDYFSDVDFAPFRGAGHFAHY
                     ERPDDANREILDFFSERTG"
     gene            2601..3593
                     /locus_tag="AC16CM_11190"
     CDS             2601..3593
                     /locus_tag="AC16CM_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3140440.1"
                     /translation="MQPSRLGTQPVGEISEVATGVFQLKVPVPIPLGFVSAYLISGPD
                     GWTLLDTGFDYPEGRATWEAGAESLGLDLRRDVSRILVSHFHPDHLGAARWLQGLSGA
                     PVYLLEQEIEHSRLVWEDDEGGSSLINGLRHNGMPGDLAVEAGSKMRADLKLPEKMLP
                     LRTGEEIPLGDGVARVVHAPGHADYQYMLHDTERGILFAADHVLLKITPNIGLWPESL
                     PHPLSRYLKSLSEMRHMNTDLVLPGHGPIFHDLGGRIDELLLHHEERLDVMYAEIQDG
                     PRTPFEVSGAVFRETLTLYERCFALAETLAHLDHLVLEGRAERFEDGALVFLAA"
     gene            3676..>5550
                     /locus_tag="AC16CM_11195"
     CDS             3676..>5550
                     /locus_tag="AC16CM_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3140441.1"
                     /translation="MPTPILATKLYVPRPQPEIVSRSRLVERLYEGLHSRLTLICAPA
                     GFGKTTVLGEFLGAIPWPAAWLSLDEEDSDPARFLAYLVAALQTIAVDIGEDALGVLE
                     SPQPPTETVLTALLNEIDAIDEDFVLVLDDYHVIDAGAVDDALSFVVEHLPLRMHLVI
                     STREDPRLPLARLRAGGQLTELRAADLRFTTDEAAEFLGGAMGLDLSAGDVLALETRT
                     EGWIAGLQLAALSLRGREDVPGFIRAFAGDNRYIVDYLLEEVLQRQPEHIRSFLLETS
                     ILDRLSGPLCDAVTGQEGGSARLEFLERGNLFVVPLDDRRLWYRYHHLFAQVLFARLL
                     AEETDRVAALHRRASGWYERNGLSADAIRHALAAEDFERAAELVELADSEMRRGRQEG
                     TLLAWLEAIPEELIRSRPVLSVGYAGVLLSTGKVEGVEDRLRDAERWLDTMADVRDRP
                     DALSTRMVVADENEYRRLPGSIAMYRAGLALALGDVSGTAKYARQVLDLATEDDHLLR
                     GAAAALLGLAFWTSGDLEAAHQSYADGMASLQRAGNISDVVGGSLALADIRIVQGRLH
                     EAMRTYEQALEIATEHGDPAMRGTADMYVGMSEIHRERGDLEAATRHLLKSKEQGEHT
                     G"
BASE COUNT         1039 a         1716 c         1823 g          972 t
ORIGIN      
        1 cagccgccaa gcgcgaggtc caagacctcg ccgacggcta cagccctccc gagcgcggag
       61 cgaacgtcta cgccgcggcc cgctctgccc gggccgccct ggaacttggc gtaaagacca
      121 tgcagtgggg ccactacgcc agcgagtacg acggcgtcat agccggccac gtagcccggg
      181 tcctcaccgg agggggtctc tcgctccccc agtgggtgcc agaggaatac ctcctcaagc
      241 tggagaaaga agcattcctg gatctcctgc aacaagaaaa gacccacgaa cgcatagaag
      301 cgctgctgac cacaggaaag ccgaagagaa attagccgtc agccgtcagc aatcagccct
      361 cagccagaaa caaagagctg accgctgaaa gcctatatct gacagcgctc gaccgaaggg
      421 agagccatga acgaagccgt catagtttcc ggggtcagga ccgccgtggg gcgtgcgccg
      481 cggggtactt tgcgggccag ccgtcccgat gacatggccg cagccgccat cggtgaggct
      541 gtgcggcgtg ccgaggggct tgccagggag gacgtggagg acgttatcat cgggtgtgct
      601 atgcccgagg ggacgcaggg gtacaacgtt gcgaggctct cttcgttgct cgcggggatg
      661 cctgattctg tgcccgcgca gacggtgaac cggttctgtg cttccgggct tcagaccata
      721 gccatggccg cggagcggat catggtcggg aacgcgaaga ctatcgttgc cggcgggacc
      781 gagcacatgt cttctacgct ggagatgccc aatttctctc ccaactcgcg gctgatggag
      841 atcaaccccg ccgcgtacat gggcatgggc ctgaccgcag agcaggtcgc ccgcgagttc
      901 gacgtgaacc gtgaggacca ggacgagttc gccctccgca gccacacgct ggcgaagaag
      961 gccgtcgagg gcggcgtctt cgacgaggag atagtgcccc tcgacgtgga gttcgactgg
     1021 gtggacgaga gcggtgaggc ccagcatgcg gagacctctt tcggacgcga cgaaggaccg
     1081 cgcgacacct cggcggagaa gctcgccaag ctaaggccag ccttccagcg cgacggcacc
     1141 gtcaccgcgg gcaactcctc acagaggagc gacggggccg ccgcggtcgt cgtcatggac
     1201 agggaggagg ccgagaggcg cgggctcacg ccgctcgccc gcttcgtcgg gttcgccgtc
     1261 ggaggcgtca ggcctgaggt catgggcata gggccgacgg tggctatccc aaaggtgttt
     1321 gagcagaccg ggctaacgat ggatgacatc gacctcatcg agttcaacga ggcgtttgcc
     1381 gcccaggccc tggctgtgat gcgtgaggtc ggcatggacc tcgacaagac caacgtccac
     1441 ggaggggcta tcgcgctggg ccatcccatg ggcgccaccg gcaccaagct cacggtgcag
     1501 ctgataaacg agatgaagcg ccgaggctcc aggtacggcc tcgtcaccat gtgcatcggc
     1561 ggcgggatgg gtgccgccgg catcttcgag aatttgcaga attagccgtc cgttttcagc
     1621 cgtcagccgt tagagaagga ctggaatgat gaacgtacct gcgtggaacc atcagttcgt
     1681 cggtgccaac gggatcgaca tccactacgt gcgtcatggc gagggttatc cgctggtgct
     1741 actgcacggg tggcccgagt tctggtacgg ctggcacaag aacatccctg ttctgtccga
     1801 gcgcttcgac gtggtcgcgc cagacctcag gggcttcggt gatacagaca agccgggtgg
     1861 tggtcctccg ggggagcttc tgggcaacct cgtggaggac ctttgcggcc tcgcggacgc
     1921 cctcggcttc gagaggttcg ggttggtctg ccacgacgtg ggcgcctacg tggggcaggg
     1981 gttcgcaagg aagtatccgg agcgtctcgg tgggttgttc ttcttcaact gtccctatcc
     2041 ggggatcggc aagcgatggg tggatccgga gagcgtcaac gagatctggt accagtcctt
     2101 caaccagcaa ccctgggcag cctccctcgt gggggagaac cggaagacgt gcgaggtcta
     2161 cttcaggcac tttctcgacc actgggcgca cgaaccgggc ctgttcgacg atgacctcga
     2221 cgcctgggtc gacaacttca tgaagccggg caacctccag ggcggcttcg actggtatat
     2281 cggatccaac gagtccaggt tggacctcat ccgcaacggc ccgcccgaga tgcccaggat
     2341 cgagacgcca acccgcatcc ggtggggaga gagcgatccc atcctgaagg tggaatgggc
     2401 cgaccggctc ggggactact tctccgacgt cgacttcgca ccctttcggg gagccggcca
     2461 cttcgcccac tacgagcggc ctgatgatgc gaaccgcgag atcctggatt tcttctccga
     2521 gaggacgggg taatcggtac ctcttgcgcc gcgggttcac cgcccacatg acagccggca
     2581 gacccgataa ccgaaccggc gtgcagccct ccaggctagg gacgcaaccc gtcggtgaga
     2641 tctccgaggt ggccactggc gtattccagc tgaaggtgcc cgtgccgatt ccgctcgggt
     2701 tcgtttcggc ttacctgatc tccggccccg acggatggac gctcctggac accggcttcg
     2761 actatcccga ggggcgggcg acgtgggagg ccggcgcgga gagcctcggg ctggaccttc
     2821 ggcgcgacgt tagccgcatt ctggttagcc actttcatcc cgaccacctc ggcgcggcgc
     2881 gatggttgca gggactatcc ggggctcccg tgtacctgct cgaacaggag atagaacact
     2941 cccggctcgt ctgggaggac gatgagggcg gctcctccct gataaatggc ctgaggcaca
     3001 acggtatgcc tggggacctg gccgtagaag ccgggtcgaa gatgagggct gacctgaagc
     3061 ttccagaaaa gatgctgccg ctgaggactg gtgaggagat accgctaggc gatggcgtgg
     3121 cgcgggtggt ccacgccccg ggtcacgccg actaccagta catgctccac gacacggagc
     3181 ggggtatcct gttcgccgcc gaccacgttc tgctcaagat cacacctaac atcggcctct
     3241 ggcccgagag cctgccgcat cctctgtcca gatacctgaa gtctctctcc gaaatgcgcc
     3301 atatgaacac tgatctggtc ctacccggac atggtcccat cttccacgac ctcggaggca
     3361 gaatcgacga gctgctactc caccacgaag agcgcctcga cgtcatgtac gcggagatcc
     3421 aggacggacc cagaacacca ttcgaggtct ccggcgccgt gttccgggaa acgctcacgc
     3481 tctacgagag atgcttcgct ctcgccgaga ctctggcgca cctcgaccac ctcgtccttg
     3541 aaggcagggc ggagcggttc gaggacgggg ccctcgtgtt cctggccgcg tagaccgcgg
     3601 gcctggaggc tgagatcata ggggtatggg tggcaagtgt gtcagaaaag tgccggagga
     3661 ctgggcggtt gatccatgcc tacgccgatt ctagcgacca aactctatgt cccccgacct
     3721 cagccagaga ttgtttcccg ttctcgcctg gtcgaacgcc tctacgaggg cctgcacagc
     3781 aggctgaccc tcatctgtgc tcctgccggc ttcggcaaga ctacggtcct cggcgaattt
     3841 ctgggtgcca taccctggcc agcggcctgg ctctcgctag acgaggagga tagcgaccca
     3901 gcgcgcttct tggcctacct cgtcgccgcc ttgcagacga tcgcggtgga tatcggggaa
     3961 gacgctctcg gtgtactcga gtcaccccag ccgccgaccg aaacggtcct cacggcgttg
     4021 ctcaacgaga tagacgccat agacgaagac ttcgtcctcg tcctcgacga ctaccacgtc
     4081 attgatgccg gagcggtaga cgatgccctc tccttcgtgg tggagcatct gcccctgcgg
     4141 atgcacctgg tcatctccac aagggaagac ccgcggctac ccctcgcccg gttgcgcgcc
     4201 gggggtcagt tgaccgagct gcgcgccgcg gacctgcggt tcaccaccga cgaagccgcc
     4261 gagtttctcg ggggagcaat gggtctggac ctctcggccg gagacgtcct cgcgctggaa
     4321 acccgtacgg agggttggat cgctggtcta cagctggcag cactctccct gcgggggcgg
     4381 gaggacgtcc ccggatttat ccgtgctttt gccggagaca atcggtacat cgtagactat
     4441 cttctagaag aggtcctcca acgtcagccc gaacacatcc gtagcttttt gctcgagacc
     4501 tctatcctgg accggctgag cggcccgctg tgcgacgctg tcaccggcca ggagggaggc
     4561 agcgcgcggt tggagttcct ggagcgaggc aacttgttcg tcgtcccgct ggacgacagg
     4621 cggctctggt accgttacca ccacctcttt gcccaggttc tatttgcacg actactggca
     4681 gaggaaaccg atcgagttgc ggctctgcac cggcgggcga gtgggtggta cgagcggaac
     4741 ggcctgagcg ccgatgcgat ccggcacgca ctcgccgcag aggacttcga acgagcggct
     4801 gagctcgtcg agctggcaga ctcagagatg cgcaggggta gacaggaggg cacgttgctt
     4861 gcctggctcg aagcgatccc cgaggagctg atccggtcca ggcccgtgct cagcgtcggg
     4921 tatgccgggg tattgcttag taccggcaag gtcgagggcg tcgaagaccg tttgcgcgac
     4981 gccgaacggt ggctggacac gatggcagac gtgcgtgatc gaccggacgc cctttccacc
     5041 cggatggtgg tcgcagacga gaacgaatac cgccgtcttc cgggttcgat cgctatgtac
     5101 cgcgccggac tggccctggc tctgggcgac gtgtcgggca ccgcgaagta tgcccgtcag
     5161 gtgctcgacc tcgcaaccga agacgaccat ctcttgcgcg gagcggctgc ggcgctcctc
     5221 ggactcgcat tctggacgag tggggacctc gaggcagccc accaatcata tgccgatggc
     5281 atggcgagtt tgcagagggc cggcaacatc tccgacgtgg tcgggggatc gcttgccctg
     5341 gctgatattc ggatagtgca aggtcgcctc cacgaggcga tgcgtaccta cgagcaggcc
     5401 ctggagattg cgaccgagca tggagacccc gcgatgcggg gaacggcaga catgtatgtg
     5461 ggtatgagcg aaatccaccg tgagcgtggt gatctggagg ccgccacgcg gcatctgctg
     5521 aaaagcaaag agcagggcga gcatacgggg
//
LOCUS       DBNGVD010000415         5618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_827610, whole genome shotgun sequence.
ACCESSION   DBNGVD010000415 DBNGVD010000000
VERSION     DBNGVD010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_827610"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC16CM_11200"
     CDS             <1..412
                     /locus_tag="AC16CM_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR3140442.1"
                     /translation="TRVLAAVEEVEPGDLGELRETLVGLAGRIVSVMMDPDYLALLRT
                     IIADTHRFPQLGEIYRDTVPERGFRVFLALMERVRERGIVDVPDAEAATRLFFGPLVT
                     YALLDGLLKPGEHPQPPEREKIEHIVDMYMKAIA"
     gene            436..2085
                     /locus_tag="AC16CM_11205"
     CDS             436..2085
                     /locus_tag="AC16CM_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3140443.1"
                     /translation="MRATEVLVIGAGPAGLTMANILARQGVPFRIIDKKGGPVRESRA
                     LVMHAKTLELLDRLGLADRAVKWGQRMGAIQVFGEGRPAGEISFFDADADDRTPYPFA
                     LVLEQDRTERLLIGGLEEAGGGVEWETELVSLAPTSDGVRATVKSGDGEEETVEVGWV
                     VGADGAGSPVRHYLGLGFEGDTYEQQLFLADVEMDWAHGSRQVSIDITRAGFYAFFPM
                     PGERRFRLIGGVPEELEGKEHITAEDVQRVLDRHSGWKTRITGVNWSSVYRTHRRMTE
                     RFRVGRVFLVGDAAHIHSPAGGQGMNTGIGDAYNLGWKLALVAKGLARASLLDSYEPE
                     RMPFARSILNGTDRAFLLQVTTDTAAQRLKILFTPLLFRIASMLPPIRRRAFWFVSQL
                     WTDYRDSPAVARSGPAGEGPQPGERAPYGFLATGGDAGRSIFEEIHGLDHDLLLFEGG
                     RSDTGLPDTVRTGEDLQALAGAYGAPIRLHVIAPGNRSLHERYGVEAPTLVLVRPDGH
                     IAYRGPAGDLNGLAAYLDRYFVGPQGREEVRRTSKGATRVR"
     gene            2290..4728
                     /gene="leuS"
                     /locus_tag="AC16CM_11210"
     CDS             2290..4728
                     /gene="leuS"
                     /locus_tag="AC16CM_11210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3140444.1"
                     /translation="MSETRQRKTYDPTALESRWQEQWAETGLYETDEDESKPKHYALT
                     MLPYPSGDLHVGHWYPMTPSDTRARFMRMNGYRVFFPIGFDAFGLPAENAAIQRGIQP
                     REWTYSNIERMRVQLRQMGAMFDFDSEVVTCDPEYYRWNQWFFIKFFEKGLAYRQEAP
                     VDWCPRDNTTLAREQVVGPDRRCERCGTPVIKKNLPQWLFKITDYAEELLDFSRIDWP
                     DRVETLQRNWIGRSEGAEIRFEIEYYGPVKVFTTRPDTLFGATFFVMSPEHPAVEQIT
                     TREQAEAVRAYVERAARMSEIDRTDATREKTGVFTGAYATNPANGEDIPVYIADYVLM
                     GYGTGAIMAVPAHDERDFEFATKHGIEIRTVISPPDLVDDQEGPYTGEGTMVNSDGFD
                     GTPSGEGKVRVTAWLGERGAGEAAITYRLHDWLISRQRYWGTPIPMIFCEECGVVPVP
                     EEDLPVLLPDESEFVPTGESPLKLDPDFYNTECPSCGGPATRETDTMDTFMDSSWYWY
                     RYLSPRYEEGPFDPARGEKWLPVDQYTGGIEHAILHLLYARFFTKVMRDLGLVDFDEP
                     MTRLRNQGIILGPDGQKMSKSRGNVVNPQEYVDRFGSDALRDFLMFIGPWSEGGPWDG
                     KGMEGVSRWLRRVLSLTDEERTAGGEADPAELTRRTNRLIKKLTEDLEAFRFNTSIAA
                     LMEHTNYLLAVKGRLDQEAWQEALQSFVKVLAPFAPHHAEEMWSELGGEYSVHQQAWP
                     GYEEDLIRAEEITLIVQVNGKLRDRIEAPADISEEAAKELALESARVRPHLEGKELRK
                     SVYVPGRLVNLVVG"
     gene            4856..>5618
                     /locus_tag="AC16CM_11215"
     CDS             4856..>5618
                     /locus_tag="AC16CM_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HIR3140445.1"
                     /translation="MEPRITRRTFLRATAGGAALAALGVTVGCDPDPSAKATAATSPV
                     GQTWNFRSRPDLRPPVITVTTPPRNTAPGLVFVAPKNGPDEAHPSQDGCVILDDEGQP
                     VWLHLLADESMDVMNFKLQEYKGEPVLTWWVGRHTGWGQGECVICDHSYSEIKRVRAG
                     NGYQADHHEFLITPQDTALITIYHGVKRDLTSVGGPADATVLDGIVQEVDIETGEVLF
                     EWHSLDHVELRQHESGFYDHLHLNSIDVFDEDHLIV"
BASE COUNT         1065 a         1781 c         1873 g          899 t
ORIGIN      
        1 gacgcgggta ttggctgccg tcgaggaggt cgagcccggg gacctcggag agttgagaga
       61 gacgctggtc gggttggcgg ggcggatcgt ctcggtcatg atggaccccg attatctggc
      121 gttattgagg accataatcg ccgacacgca ccggttcccc cagctgggag agatctaccg
      181 cgatacggtt cccgagcgcg ggttcagggt cttcctggcg ctcatggagc gcgtgcggga
      241 gagggggatc gtggacgttc cagacgccga ggcggcgacg aggttgttct tcgggccgct
      301 cgtgacctac gccctcctcg acggccttct caagcccggt gaacatccgc agccgccgga
      361 gcgggagaag atcgaacaca tcgtggacat gtacatgaag gccatcgcct gaataggggc
      421 gtgaaaggag caggcatgcg tgctacggaa gtgctcgtga tcggtgcggg gccagcgggg
      481 ctcaccatgg cgaacattct cgccaggcag ggcgttcctt tcaggatcat cgacaagaaa
      541 ggtggtcctg tcagggagag cagggcgctc gtgatgcatg ccaagacgct ggagctcctc
      601 gacaggctcg gtctggctga tcgggccgtc aagtggggcc agaggatggg ggccatacag
      661 gtcttcggcg agggcagacc cgccggtgag atctccttct tcgacgccga cgccgacgac
      721 cgcacgccct acccgttcgc cctcgtcctg gagcaggaca ggacggagcg gctcctgatc
      781 ggcggcctcg aagaggctgg gggcggggtc gagtgggaga cggagctcgt ctcgctagct
      841 cccacgtcgg acggcgtgcg ggcaaccgtg aagtccgggg atggagagga ggagacggtc
      901 gaggtcggat gggtcgtcgg cgctgacggg gcgggcagcc ccgtgaggca ctacctcggt
      961 ctcggcttcg agggtgacac ctacgagcag cagctgttcc tggcggacgt agagatggac
     1021 tgggcgcacg gctcgcggca ggtgagcata gacataactc gcgcggggtt ctacgcgttc
     1081 ttccccatgc ccggggagcg gcgctttcgt cttatagggg gtgtgccaga ggagctggag
     1141 gggaaagagc acatcacggc cgaggatgtg cagcgcgtcc tcgaccggca cagcggttgg
     1201 aagaccagga tcaccggcgt gaactggagc agcgtctacc gcacccaccg caggatgacc
     1261 gagcgcttca gggtcggccg cgtgttcctc gttggcgacg ccgcccacat ccactcgccg
     1321 gctgggggac agggcatgaa cacgggcatc ggcgacgcct acaacctggg atggaagctc
     1381 gcgctcgtcg cgaagggcct cgcccgtgcg tcgctgctcg attcctacga gcccgagcgt
     1441 atgccgtttg cccggtcaat cctcaacgga accgacaggg ccttccttct gcaggttacg
     1501 accgacacgg ccgcgcagag gctcaagatc ctcttcacgc cattgctctt ccgaatcgcg
     1561 tcgatgcttc cgccgatcag gaggcgggcc ttctggttcg tctcccagct ctggacggac
     1621 taccgggata gccccgccgt cgcaagatcc gggccggcgg gggagggacc tcagcccgga
     1681 gaacgcgctc cttacggctt cctcgccacg ggcggggatg ccggtcgcag catcttcgag
     1741 gagatccacg gtctcgacca cgacctgctc ctcttcgaag gggggagatc cgacacaggg
     1801 ttgcccgata ccgtacggac aggcgaggat ctgcaagcac tggccggcgc gtacggggcg
     1861 cccatccggt tgcacgtgat cgccccggga aacagaagcc tccacgagcg ttacggcgtg
     1921 gaagcgccca ccctggttct ggtcaggccc gacgggcata tcgcataccg cggtcctgcg
     1981 ggggacctca acgggctagc agcctacctc gaccggtatt tcgtgggccc ccaggggcgc
     2041 gaagaggtca ggagaaccag caaaggagcc acgagggtaa ggtagatccc gcacgctccg
     2101 gtatcggcac gccgccaggg acgccatcct ggcggcgttt cccttttgta gagggtgttc
     2161 ttggtgctat attaggcgta tgtgcgccag ggatcacaag gttcagaggt cggactcccg
     2221 actatagagc cctgcgccgg cttttcccga tctagaacga atcctggtct gtaaccgaag
     2281 gagtctcacg tgagcgagac gagacagaga aagacatacg atcccaccgc cctcgaaagc
     2341 cgctggcagg agcagtgggc cgagaccggc ctttacgaga ccgacgagga cgagagcaag
     2401 ccgaagcatt acgcgctcac catgctcccc tacccgagcg gggacctgca cgtcgggcac
     2461 tggtacccga tgacgccgtc ggatacgcgg gcccgcttca tgcgcatgaa cggctatcgg
     2521 gttttctttc caatcggctt cgacgccttc gggcttccgg ccgagaacgc cgccatccag
     2581 cgcggcatcc agccccgtga gtggacctac tccaacatcg agcggatgcg cgtgcagctc
     2641 aggcagatgg gcgccatgtt cgacttcgac tcggaggtcg ttacctgcga ccccgagtac
     2701 taccgctgga accagtggtt ctttatcaag ttcttcgaga aagggctggc ctaccgtcag
     2761 gaggcgcccg tcgactggtg ccccagggac aacaccacgc tggcgcggga gcaggtcgtg
     2821 ggccccgaca ggcgctgcga gcggtgcggg acgccggtca taaagaagaa cctgccacag
     2881 tggctcttca agatcacgga ctacgccgag gagctcctcg acttctcccg catcgactgg
     2941 ccagatcgcg tcgagaccct tcagcggaac tggatcgggc gctccgaggg cgccgagata
     3001 cggttcgaga tcgagtacta cggccccgtc aaggtcttca cgacccgccc ggacacgctc
     3061 ttcggtgcca cgttcttcgt gatgagcccg gagcatccgg ccgtggaaca gataacgacc
     3121 cgggagcagg ccgaggcggt gcgcgcctac gtggagcggg ccgcgaggat gagcgagatt
     3181 gaccgcaccg acgcgaccag ggagaagacc ggcgtcttta ccggggccta cgcaaccaac
     3241 ccagcgaacg gggaggacat ccccgtctac atcgccgact acgtgctgat gggctacggg
     3301 acgggcgcga tcatggccgt cccggcccac gacgagcggg acttcgagtt cgcgacgaag
     3361 cacggcatcg agataaggac cgtgatctca cctcctgacc tcgtcgatga ccaggagggg
     3421 ccctacaccg gagagggcac gatggtcaac tccgacgggt tcgacggcac cccgagcggc
     3481 gagggcaagg tgcgggtgac ggcctggctc ggagagcgcg gagccggcga ggccgcgatc
     3541 acctacaggc ttcacgactg gctcatcagt cgacagcgct actggggaac cccgataccg
     3601 atgatcttct gcgaggagtg cggcgtggtc cccgtcccgg aagaggatct cccggttctc
     3661 ctccccgacg agtccgagtt cgtgccaacg ggtgagtctc ccctgaagct cgaccccgac
     3721 ttctacaaca cggagtgccc cagctgcggt ggcccggcca cgcgcgagac agacaccatg
     3781 gacacgttca tggactcctc gtggtactgg taccgttacc tctcaccccg ctacgaagag
     3841 gggcccttcg accccgcgcg gggggagaag tggctccccg tcgaccagta caccggcggc
     3901 atcgagcacg ccatactgca cctgctctac gcccgcttct ttaccaaggt gatgagggac
     3961 ctcgggctcg tcgacttcga cgagccgatg acccgcctgc gtaaccaggg gatcatactc
     4021 gggcccgacg ggcagaagat gagcaagagc cgcggcaacg tggtcaaccc ccaggagtac
     4081 gtggaccgct tcggctccga cgcgctccgg gattttctga tgttcatcgg cccctggagc
     4141 gaggggggac cctgggacgg gaagggcatg gagggcgtct cacgctggct caggcgcgtg
     4201 ctctcgctca ccgacgaaga gcgaaccgcc ggcggcgagg ccgatcctgc tgagctgacg
     4261 cgccggacga accgcctcat caagaagctt accgaggacc tcgaggcctt ccgcttcaac
     4321 acctctatag ccgcgctcat ggagcacacc aactacctgc ttgcggtcaa gggacgcctc
     4381 gatcaggagg cctggcagga ggccttacag agcttcgtca aggttctcgc cccgttcgcc
     4441 ccgcaccacg cagaggagat gtggtccgag ctcggtgggg agtactctgt tcaccagcag
     4501 gcatggccgg gttacgagga ggacctgatc cgggccgagg agatcacgct catagtccag
     4561 gtgaacggca agcttcgcga ccgtatcgag gcgcctgcgg acatctccga ggaggccgca
     4621 aaggagctgg cgctggagag cgcgcgggtc cgtccccacc tcgaaggcaa ggagctccgc
     4681 aagagcgtct acgtgccggg ccggctcgtc aacctcgtgg tagggtagcg aagccccggc
     4741 aaccgagata tccctcgtgg accctggggc gaagtgcgaa acaaaatggt gtaacttgcg
     4801 taactctggg agtctggcgc gcaagccggg acggtagcta gaaagaggcg gtggtttgga
     4861 acccaggatc acacgacgaa cgtttttgag ggccacggcc gggggcgccg cgctggccgc
     4921 gctcggtgtt acggtgggct gcgaccccga cccgagcgcc aaagcgaccg ccgccacatc
     4981 tccggtaggg cagacgtgga acttccgctc gcgcccggac ctccgaccgc cggtaataac
     5041 cgtgaccacg ccccccagga ataccgcccc cggactggtc ttcgtcgccc cgaaaaacgg
     5101 ccccgacgag gcccatccca gccaggacgg gtgcgtgatc ctggatgacg aaggccagcc
     5161 cgtgtggctg cacctgctgg cggacgagtc catggacgtt atgaacttca agctgcagga
     5221 gtacaagggt gagcccgtcc taacctggtg ggttggccgc cacaccggct ggggccaggg
     5281 cgaatgtgtc atctgcgacc attcctacag cgagataaaa cgcgtgaggg ccggcaacgg
     5341 ctatcaggcg gaccaccacg agttcctgat caccccccag gacaccgccc tgatcaccat
     5401 ctatcacggg gtgaaacggg atcttacctc cgtgggcggc cccgccgacg ccacggtgct
     5461 cgacggcata gtccaggagg tagatatcga gaccggcgag gtcctcttcg agtggcacag
     5521 cctggaccac gtcgagctcc gccaacacga gtcgggcttc tacgatcacc tgcacctgaa
     5581 ctccatagac gtcttcgacg aggaccacct gatcgtct
//
LOCUS       DBNGVD010000416         2226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1920520, whole genome shotgun sequence.
ACCESSION   DBNGVD010000416 DBNGVD010000000
VERSION     DBNGVD010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1920520"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="AC16CM_11220"
     CDS             complement(<1..842)
                     /locus_tag="AC16CM_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3140446.1"
                     /translation="MVDEKVGKLARVLVDYSIEAGEGDQVVVFGEVGAQPLITALYAR
                     LLEVGAFPVLQVGLPGTQETFFDKAQDVHYERIPSIRRFVAEEADAHIAVRAPSNTRA
                     LSNVDPRKQQALAELNKPLQDIMLKRNRWVLTLFPTEALAQEAGMGLAEYEAFAFGAM
                     GLDEGDPVRYWKDKSREQERLKERLQEAREIRIVGPETDLTLSVEGRTFINSAGRRNM
                     PCGEVFTGPIEDSANGTVYFGVPAAIGGREVSGARLRFEGGKVVEASAERGDDFLQSL
                     LDTDP"
     gene            complement(915..1691)
                     /locus_tag="AC16CM_11225"
     CDS             complement(915..1691)
                     /locus_tag="AC16CM_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3140447.1"
                     /translation="MTIPVIFSTGSLYPFGLERTYAWAAEAGFDGVEIMMDERWDTHQ
                     ADYLRSLVERYGVPVKAFHTPLYRGAWGLGPEVTLVRVARLAVKMDVPVVVAHPPPPG
                     RPLERWREGPLREAREQGAVVAVENMPRNSTGGLFSVRRKTCYLPEHLSDVGEVTLDT
                     SHAGASKVDLLRAHSVLAAQLRHVHLSDSNLQAGRDEHRLPGKGRLPLKPFLAALATG
                     GYPGAVSLELKPWPLGTPDPGTILDRMRAALEFTRDGLAI"
     gene            complement(1688..>2226)
                     /locus_tag="AC16CM_11230"
     CDS             complement(1688..>2226)
                     /locus_tag="AC16CM_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3140448.1"
                     /translation="SQEITHEQNLVNVEAETENFVRTMAGLGDRLGPLLLQLPPSFTV
                     EGMGVLEDFLKALPEGFRYAVEVRHRSWLGSDLPVMLRERGVALTLIDYPRMPRMDQA
                     TADFVYIRWLGNRREFPEGHTRLKKERDEDLRWWSNLVGRFLEEGRTVFAYANNHYQN
                     HSPSTLARFLEIRRGGSG"
BASE COUNT          390 a          743 c          693 g          400 t
ORIGIN      
        1 gggtcagtgt cgagcaggct ctgcagaaaa tcatcgcccc gctccgcgct ggcttcgacg
       61 accttgccgc cttcgaagcg aagcctcgcg cctgagacct cgcgaccccc aatcgcggcg
      121 ggcacgccga aatataccgt tccgttggca gaatcctcga tggggccggt gaacacctcg
      181 ccgcaaggca tgttgcgccg gcccgccgag ttgatgaacg ttcgtccctc gaccgagagc
      241 gtcaggtccg tctcggggcc cacgatacgg atctcgcgcg cctcctggag cctttccttc
      301 aaccgctctt gctcacgcga tttgtccttc cagtagcgta cggggtcccc ttcgtcgaga
      361 cccatcgccc cgaaggcgaa cgcctcgtac tcggcgaggc ccatgccggc ctcctgggcg
      421 agagcctctg tggggaagag cgtcagtacc catcggtttc tctttagcat gatgtcctgg
      481 aggggcttgt tcagttcggc gagggcctgc tgcttgcgcg ggtccacgtt ggacagcgca
      541 cgcgtattcg acggggccct taccgcaatg tgggcgtcgg cctcctctgc cacgaagcgc
      601 cgtatcgagg gtatccgctc gtagtggaca tcctgcgcct tgtcgaagaa cgtctcctgg
      661 gtaccaggca accccacctg cagcacgggg aaagccccga cctcaagcaa ccgggcgtag
      721 agcgcggtaa tgaggggctg agccccgacc tcaccgaaga cgacgacctg atctccctcc
      781 cccgcctcga tggagtagtc aaccagcacc cgcgccaact tgccgacctt ctcgtctacc
      841 atccacggct ccttctcggg gattatcctg cagagcttat catccaggaa cgccaccaaa
      901 ttacggggca cgacctagat ggcgaggccg tcacgcgtga actccagggc cgcgcgcatg
      961 cggtccagga tcgtaccggg atccggggtc cccaggggcc atggtttcag ctccaggctg
     1021 acggcgccgg gatacccgcc ggtggccagc gccgcaagga acggtttcaa gggcagacgg
     1081 cccttgcccg gaagccggtg ttcatcccga cccgcctgga ggttcgagtc ggatagatga
     1141 acgtgacgca gttgcgcggc gaggacagaa tgggcccgga gaaggtccac cttgctagcc
     1201 ccggcgtggc tggtgtcaag cgtgacctcg ccaacgtccg acaggtgctc gggaaggtag
     1261 caggtcttgc gccttacgct gaacaggccg ccggtgctgt tgcggggcat gttctcgacg
     1321 gccaccacgg ctccctgctc ccgcgcctca cgcagcgggc cttccctcca gcgctccagc
     1381 ggcctgccgg gcggcggggg atgtgccacc accaccggaa catccatctt gacggcaagc
     1441 ctggcgaccc gcaccagggt cacctccggc cccaaccccc acgcaccgcg gtagagcggg
     1501 gtatggaagg ccttgaccgg tacgccgtat cgttcgacaa ggctccgcag gtagtctgcc
     1561 tggtgggtgt cccagcgttc gtccatcatg atctcgaccc cgtcgaaacc cgcctcggca
     1621 gcccaggcgt acgtcctctc caggccgaac ggataaaggg atcccgtgct gaatatgacg
     1681 ggtatcgtca acccgaccct ccgcgccgga tctccagaaa acgggccagc gtcgatgggg
     1741 agtggttctg gtagtggttg ttggcgtagg cgaagaccgt cctgccctcc tccagaaagc
     1801 ggcccaccag attagaccac caccggagat cttcgtcgcg ctctttcttg agacgggtgt
     1861 gtccctcggg gaactcgcgg cggttgccga gccagcggat gtagacgaag tccgccgtcg
     1921 cctgatccat cctgggcatc cttggatagt cgataagcgt cagggcgacg ccgcgctcgc
     1981 gtagcatcac cgggaggtcc gagcccagcc agcttcgatg ccttacctcg acggcgtacc
     2041 ggaagccttc tggaagcgcc ttaaggaagt cttcaaggac acccatcccc tcgacggtga
     2101 aggagggcgg cagctgaagg agaaggggtc cgagcctgtc gccgagaccg gccatggtgc
     2161 gtacaaagtt ctcggtctcg gcttcgacat tgaccagatt ctgttcgtgg gttatctctt
     2221 gggagt
//
LOCUS       DBNGVD010000417         3880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1192079, whole genome shotgun sequence.
ACCESSION   DBNGVD010000417 DBNGVD010000000
VERSION     DBNGVD010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1192079"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..425)
                     /locus_tag="AC16CM_11235"
     CDS             complement(63..425)
                     /locus_tag="AC16CM_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HIR3140449.1"
                     /translation="MDHRPPSIKVDKPWGRFEQYTHNLPCTVKIITVAAGGKLSSQYH
                     HHRDELWVVLDKGVRVHLGEDVLEPEVGEKIFVPRETVHRLEGSEDGEVRIMEISFGE
                     FDEEDIVRIDDVYGRVDP"
     gene            570..935
                     /locus_tag="AC16CM_11240"
     CDS             570..935
                     /locus_tag="AC16CM_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3140450.1"
                     /translation="MPRIEVEDAGTFDVEEGKRLVLAIEEDAGVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTRAELEVLESRNLLGEVRLSCQALCDHDVKVRVLMTVESTGLDGP
                     GPQPEPEITPEPEWVDKPV"
     gene            complement(994..1962)
                     /locus_tag="AC16CM_11245"
     CDS             complement(994..1962)
                     /locus_tag="AC16CM_11245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3140451.1"
                     /translation="MAVRFGVFVPQGWKMDLVDIADPFEQYEAMTRVARVAEETGSYD
                     SVWVYDHFHTVPEPTMNTVFEAWSACAGLARDTREVKIGQMVTCNGYRNPALLAKIAS
                     TVDVMSNGRLLCGLGAGWYEHEWLAYGYGFPEIPVRMRMFKEACEIVHRMFTEDGPTF
                     DGEFYSIDRPINEPKGVSKPHPPIWIGGGGEKVTLRLVARWGDASNFGNGDVETIRRK
                     IEVLEGHCENEGRDFGEITRSTSINVHLLEDGEDPERATREARGSRSLQEYSQEYLVG
                     TPAEIIGRLHPMVEAGIDYFITYLPRVAYDPTPVESFAHEVIPNVG"
     gene            complement(2219..2749)
                     /locus_tag="AC16CM_11250"
     CDS             complement(2219..2749)
                     /locus_tag="AC16CM_11250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3140452.1"
                     /translation="MSDKDSNPPISTGGGDDGTTTLLGSGRMGKDDPKIVVVGDLDEA
                     SSFLGLARAEAEGEMSGLVIELQRLLYRVMGDVAMPKEDNLVGEEDLKLLDDALEHWR
                     SRTEIPREFVVPGESRPGALLDVARTVTRRAERSLVAAGYATGHPYAIRVVNRLSDLL
                     FVLARNADGEKTLSKG"
     gene            complement(2827..>3880)
                     /locus_tag="AC16CM_11255"
     CDS             complement(2827..>3880)
                     /locus_tag="AC16CM_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3140453.1"
                     /translation="LLARQNLSAFDAIERLAGMQAQEPEAPHVGLWARLEGFDPGELS
                     GLISDRRVVRGPLMRSTVHLVSGRDCLTFRPIIQPVLERAFSGSPFRRELEGVDLDEL
                     IGDGRALLEERPLTRAGLSRLLAERWPDLDPLSLAYAVTFLTPLVQVPPRGIWGQSGQ
                     ATWTTTESWLGSPLDPDPSPDGVFLRYLAAFGPATVADARAWSSLGGLREVFERLRPR
                     LVTFRDERGRELFDVPGAPLPAPDTVAPPRFLQAFDNALLSHHDRTRIISDENRGALT
                     RDRSMRGVLLDGFACATWRTEKENGGWTLVISPFRPLTGGDREELAREGGRLLDFLAR
                     DHEVDEFTVRFEGGEV"
BASE COUNT          695 a         1275 c         1215 g          695 t
ORIGIN      
        1 gcggacgatt cctcgtgccg aggatcctct tcttccctga aatccaacgg agaactacac
       61 cgctatgggt cgacgcggcc gtacacgtcg tcgatcctta cgatgtcctc ctcgtcgaac
      121 tcgccgaagg agatctccat tatcctgacc tccccgtctt cggaaccttc gagcctgtga
      181 acggtctccc gcgggacgaa gatcttctcc ccgacctcgg gttcaagaac gtcctcgccg
      241 agatgcaccc gtaccccctt gtcgagcacg acccacagct cgtcgcggtg gtggtggtac
      301 tggctggaga gcttgccgcc ggcggcgacc gtgatgatct ttaccgtgca ggggaggttg
      361 tgagtgtact gttcgaagcg tccccagggt ttgtctacct ttatagaagg cgggcggtga
      421 tccaaaacgt ccccctttcg tgaacgaaga gtttagccta tccaacctcg ctttgggaga
      481 agcaagatat cacgagcccg cgccgggacg ttcgcgcctg ctcgcgtggc gtggttagat
      541 gtggcggtcg tctacaggag ggaaggccag tgccaaggat cgaggtagaa gacgccggta
      601 cgttcgacgt agaggagggc aagcggctgg tcctcgccat cgaggaggac gctggggtgg
      661 atatcctgca ccgttgcgga tcttatgcca gatgcacgac gtgcaggata gagtacctgg
      721 agggcgaacc ggagaagatg acgagggccg aactcgaggt gctagagagc cggaacctgc
      781 tcggcgaggt gcgtctctcg tgccaggcgc tctgcgacca tgacgtgaag gtccgcgtcc
      841 taatgaccgt ggagtcaacc ggtctcgacg gcccgggccc gcaacccgaa cccgagatca
      901 ccccggagcc ggaatgggtt gataaacccg tctgactcgt gcccgtgggg tggttcgtag
      961 ttgggatgtc gggaatcgtt ggtcgggtgg gttctatccg acgttgggga tgacctcgtg
     1021 ggcgaagctc tctaccggcg tcgggtcgta ggctacgcgt ggcaggtagg ttatgaagta
     1081 gtcgataccg gcctcgacca tcgggtggag tcgcccgatg atctccgccg gcgtgcctac
     1141 caggtactcc tgggagtatt cttggaggct gcgggagccg cgggcctctc tagtcgcccg
     1201 ttcggggtct tccccgtctt ccagtaggtg gacgttgatg gaggtggagc gggtgatctc
     1261 cccgaagtcc ctgccctcgt tctcgcagtg gccttcgagc acctctatct tccgccttat
     1321 ggtctcgacg tcaccgttgc cgaagttgct ggcgtcgccc caccgggcga cgagcctcag
     1381 ggtaaccttc tcgccgccgc ccccgatcca gatcggtggg tgtggcttgc tgacgccctt
     1441 tggctcgttg atgggcctgt ctatgctgta gaactcaccg tcgaaggtgg ggccgtcctc
     1501 ggtgaacatg cggtgaacga tctcgcacgc ctccttgaac atacgcatcc tgacggggat
     1561 ctcggggaag ccgtagccgt aggcgagcca ctcgtgctcg taccagccgg cccccaggcc
     1621 gcatagcaac cgtccgttgc tcatcacgtc caccgtcgag gcgatcttgg ccaggagcgc
     1681 cgggttgcgg tagccgttgc aggtgaccat ctgcccgatc ttgacctctc tagtgtctcg
     1741 cgccaggccc gcgcacgcgg accacgcctc gaagacggtg ttcatggtcg gctcgggaac
     1801 ggtatgaaag tggtcgtaga cccatacaga gtcgtaggat ccggtctctt ccgcgaccct
     1861 cgctacccgc gtcattgcct catactgctc gaatggatcc gcaatgtcga ccagatccat
     1921 cttccaaccc tgcggaacga agaccccaaa acgcacggcc atatatcccc caatttcttg
     1981 tacggacgaa cgccaagcat attcccgccc cattccccac gcaaccaacg atcaacaatt
     2041 cccacgaccc tgtaggggcg tttcacgaac cacgcgacaa acaaccgccc accggccccg
     2101 aacctcgcca accagacgta ggggcgtgat cgaaacccgc ccaacgacga accgctgatt
     2161 cccgacaccc ccacaaccct gtaggggcgg ttcgcgaacc gcccaacgaa gtcccgtcct
     2221 accccttcga gagggtcttc tccccgtcgg cgttgcgggc gaggacgaag aggaggtccg
     2281 agaggcggtt cacgacgcgt atggcgtagg ggtgtcctgt ggcgtacccg gcggcgacga
     2341 ggctgcgctc cgcgcggcgc gtaacggtgc gtgccacgtc cagcagcgca ccaggcctgc
     2401 tctccccggg gacaacgaac tcccttggga tctccgtcct ggagcgccag tgttcgaggg
     2461 cgtcgtcgag gagcttcagg tcttcctcgc cgaccaggtt atcctctttc ggcatcgcca
     2521 cgtcgcccat cacgcggtac aggaggcgct ggagctcgat cacgagcccg gacatctccc
     2581 cttcggcctc ggcacgggcg aggccgagaa acgagctggc ctcgtccagg tcaccgacca
     2641 ctacgatctt cgggtcgtcc ttgcccatcc ttcccgaccc gagcagcgtc gtcgtcccgt
     2701 cgtcgccgcc gcccgtcgaa atcggtgggt tgctgtcctt atcgctcacg aggtctctcc
     2761 ctctctccga tgtgcgagac tgattatccc gaagaccggg acggcgcaaa ccgggagacc
     2821 gcaagcctac acctcacctc cctcgaaccg gaccgtgaat tcatcgactt cgtgatctct
     2881 cgccagaaag tcgagcaggc gccctccctc cctggctagc tcctcacggt ccccccccgt
     2941 caggggccgg aagggtgaga tcacgagcgt ccatccccca ttctctttct cggttctcca
     3001 ggtcgcgcac gcaaagccat cgagcagcac gccgcgcatc gagcggtccc tggtcaatgc
     3061 cccgcggttc tcgtccgaga tgatgcgggt tctgtcgtgg tgggagagca acgcgttgtc
     3121 gaaggcctgc aagaagcgcg gtggggccac ggtgtcgggg gcaggtaacg gggcgccagg
     3181 tacatcgaac agctcgcgtc cgcgctcgtc gcggaaggtg acgagccgcg gcctgagacg
     3241 ctcgaagacc tcccgtaaac cgccaaggct cgaccacgcc ctcgcatcgg cgacggtggc
     3301 cggcccgaag gccgccaggt agcgaaggaa caccccatcc ggcgagggat ccgggtcgag
     3361 aggactgccc agccaggact cggtcgtggt ccaggtggcc tgcccgctct ggccccagat
     3421 gccacgcggc ggtacctgta ccagcggcgt gagaaaggtg acggcatagg cgagcgagag
     3481 aggatcaaga tccggccacc tctccgccaa caaccggctc aacccggcgc gggtgagcgg
     3541 gcgttcctcg agcaaagcac gaccgtcgcc gataagctcg tccaggtcga cgccttcaag
     3601 ctcgcggcgg aaggggctac cggagaaggc gcgctcgagg accggctgta tgatggggcg
     3661 aaaagtcagg cagtcacgcc cggagaccag gtggacggtc gagcgcatca gcgggcctcg
     3721 caccaccctg cggtcggaga tcaggccgga cagctccccg ggatcgaagc cctccagccg
     3781 cgcccagagc ccgacgtggg gcgcctcggg ctcctgggcc tgcatgccag ccaaccgctc
     3841 gatggcgtcg aaggctgaca ggttctggcg tgccaacaac
//
LOCUS       DBNGVD010000418         3280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_98451, whole genome shotgun sequence.
ACCESSION   DBNGVD010000418 DBNGVD010000000
VERSION     DBNGVD010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_98451"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..396
                     /locus_tag="AC16CM_11260"
     CDS             94..396
                     /locus_tag="AC16CM_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3140454.1"
                     /translation="MACGDTGAPLQADHVVPLTRGGSNDIGNIQPLCGACNRKKFVSV
                     IDYRTGWLAEETVAVGAGCYRLSAPRLWLMAEASRPLAPRYRAVPSGERRVRYARP"
     gene            complement(323..1015)
                     /locus_tag="AC16CM_11265"
     CDS             complement(323..1015)
                     /locus_tag="AC16CM_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3140455.1"
                     /translation="MESVPRSSEGSDPIRVDSVADLAYGRIRGYVLGGEVPPGARLGQ
                     VELAERFGISRTPVREALRRLAGEGLVDFHSNRGFRVADLGLDAVLRRMEVRSLVEPG
                     IARLAAGRRTEEDVRRLQEVIASQEEARDGISAHDASRDFHMALARASGNEELVRVLN
                     SLWLVEVGRRLLARRTADPNWLHDDVSEHRGILAAVKEGHAAEAERLMAEHIGRAVHH
                     WEPLGSGGPGGD"
     gene            1291..2208
                     /locus_tag="AC16CM_11270"
     CDS             1291..2208
                     /locus_tag="AC16CM_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HIR3140456.1"
                     /translation="MTGTKRPKLSYLICATPRSGSTLLSDALGRTGIAGYPAEHFEVL
                     LETGHPRRPRDYFRRSNDPAVWALLDDPAFRDVLGGDEGRFVEGPPSEDISWRPPDFG
                     RLLDLALERGTTPNGVFGTKIMWAYFRDFVRLARRSGREDVGPYEVPASVFPHLRRHV
                     WIRRRDTLRQAVSLWKALQTQRWRRDSDEEVGGRDLRFSFAAIDHLRLRIDEHNAAWE
                     DYFEGCGVEVVRVLYEDLLEDYQDTTLRLLREIGTSIPEGFAVGNPRMKLQANEHSEE
                     WVRRYEELARRRQLDRQHDRVAAGTFKSA"
     gene            2602..3057
                     /locus_tag="AC16CM_11275"
     CDS             2602..3057
                     /locus_tag="AC16CM_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR3140457.1"
                     /translation="MMRRILMVVLMVAIGALVLAPAAFAQDDNPSGDDMRGDDRGGER
                     AFDDNPSADDLREDDRDDVRGFDDNPTGDDGSASATSSATASASSSATPSASPSASAT
                     ASATPSASPVADDSVLPETGGVTPLALVAGILLLGSGIGAVMLMRRRTS"
BASE COUNT          579 a         1045 c         1084 g          572 t
ORIGIN      
        1 ggccgcaaga ggagggcggc gaagcatgcc agcccgggta acttcaccgt cgaggatttc
       61 gaagctctat gcgaaaggta tggcaacagg tgtctggctt gcggtgacac cggggccccg
      121 ttgcaggctg accacgtggt gcctctaaca aggggcggct cgaacgacat cggcaacatc
      181 cagccgttgt gcggggcgtg caacaggaag aagttcgtga gcgtcatcga ctacagaacg
      241 gggtggcttg cagaggaaac ggttgccgtg ggggcgggct gttaccggct ttcggctccc
      301 cgtctctggt tgatggccga ggctagtcgc cccctggccc cccgctaccg agcggttccc
      361 agtggtgaac ggcgcgtccg atatgctcgg ccatgaggcg ttcggcctct gcggcgtgac
      421 cttctttaac ggcggcaagg atcccacggt gttccgagac gtcatcgtgc agccagttgg
      481 ggtcggcggt gcgcctggcg aggagtcgcc ttcccacttc gacgagccag agcgagttca
      541 ggacccgcac gagctcttcg ttgccagagg cgcgggccag cgccatgtgg aagtcgcggc
      601 ttgcgtcgtg ggcggagatc ccatctctcg cctcctcctg gctcgcgatg acctcctgga
      661 gacgtcgaac gtcttcttcc gtcctccgcc cggccgcgag acgcgcgatg cccggctcca
      721 ccagggaccg cacctccatg cgtcgcagca cggcgtccaa ccccaggtca gcgacccgga
      781 aaccccggtt ggagtggaaa tctaccaggc cctcgccggc cagccgccgc aaggcctcgc
      841 gcacgggggt gcgtgagatg ccaaacctct ccgccagctc cacctgacca agccgcgcac
      901 ccggcggaac ctccccacca agcacgtatc cccttatccg cccgtaggca aggtcagcca
      961 cagagtcaac ccgaatgggg tcgctcccct ctgacgaacg cggtacggat tccatcgccc
     1021 gatcatagcc cttgtggata cattatgcaa tgtagcggta ggtgtcggat cacgggcatc
     1081 aggctacagg aagtcaaagg tctgacgccc tcctgctggg tggctcgtct ggtacttctg
     1141 ggtggggatc ggcgtagatg atccttgtcg agtttgtcta gctattcggt agagtatatt
     1201 gtagggtgtt acgcctgttg acgagcagtt atgggtttgg gaccgtggcc gcccttcccg
     1261 gcccgtggca ccggacggag gaggttcttg atgaccggta cgaagaggcc gaagctctcc
     1321 tacctgatct gtgccacgcc ccggagcggt agcacgctct tgagtgacgc gctggggagg
     1381 accgggatcg ccggttatcc cgcggagcac ttcgaggtct tgctcgagac gggccacccg
     1441 cggcgtccgc gcgattactt ccggcgctcc aacgacccgg cggtatgggc gctcctggac
     1501 gaccctgcgt ttcgcgacgt actcggcggg gatgagggca ggttcgtcga gggtccgcct
     1561 tctgaggaca tctcctggcg gccgcccgat ttcgggaggc tgctcgatct tgcgcttgaa
     1621 cgaggcacca ctcccaacgg cgttttcggc accaagatca tgtgggccta cttccgcgat
     1681 ttcgtgcgcc ttgcgcgcag gtccgggcgc gaggacgtcg ggccatacga ggtgccggcc
     1741 tccgtcttcc cccacctgcg caggcacgtg tggatccgcc ggcgggatac cctgcgccag
     1801 gccgtctcgc tgtggaaggc gctccagacc cagaggtggc gcagggactc cgacgaggag
     1861 gtgggcgggc gggacctgcg tttcagcttc gcggcgatcg accacctcag gctgcgcatc
     1921 gacgagcaca acgcggcctg ggaggactac ttcgagggct gcggcgtgga ggtggtccgc
     1981 gttctatacg aggacctcct cgaggactat caggacacga cgctccggct gctacgagag
     2041 atcggcacaa gtattcccga gggcttcgcc gtgggaaacc cgaggatgaa gctgcaggcc
     2101 aacgagcact cggaggagtg ggtgcgccgc tacgaggagt tggcccgccg gcgccagcta
     2161 gaccgccagc acgaccgcgt ggccgccggt accttcaaaa gcgcgtgagc gaccatcgta
     2221 aggagaagga acggccctcg ccagggacga accacggttc cgtccaagcg cgcggcttcc
     2281 agtgtggctt caacgccgag aatggtgttt attgctgaca ggatcccccg caataattca
     2341 tcgagaagca agcatctttc gtggccccag acgcgacacc agaggaccgt aggaccaacc
     2401 gagacggttg cccttcggta agccccctgc aagatgcgtc ccacgcggag catccgagcg
     2461 aggcagacgt ctcccgggcc ttgaggctgg gaatttgatc tttatcgtat tgttatcgac
     2521 cgttgtccgc cctgtaaccc ccgctcgtcc gcgcgatgtt aggttgaaat agccatgcaa
     2581 ccgaagcgca cagagaggaa gatgatgcgt aggatcttga tggtggtgct gatggtggcg
     2641 attggggcgc tggttctcgc gccggcggcc ttcgcccagg acgacaaccc tagcggggat
     2701 gacatgagag gcgacgaccg cggtggagaa cgggccttcg acgacaaccc cagtgcggac
     2761 gatctcaggg aggacgatcg cgacgatgtt cgcggtttcg atgataaccc caccggcgac
     2821 gatggatctg cgagcgccac gtcgagcgcc acggccagcg cctcatcgag cgccacaccg
     2881 agcgccagcc cctctgcctc tgccacggcg agcgccactc ccagcgcgtc ccctgtggct
     2941 gacgatagcg tgctgcctga gaccgggggc gtgaccccgc tggcgctggt cgccgggatc
     3001 ctgctgctcg gatcggggat cggggccgtc atgctgatga ggcgccgcac ctcgtagtcc
     3061 gcaggtcgag accctcttca cgagcacgtt gggccggaga tccgccaggg tctctggccc
     3121 ggccctttga cccgccagcg agccctgcaa aacacccgcc aacgccgaac cggacaacac
     3181 ggcaagggga tacggataga atgagcatca tgcaacgatt gctcctcggt gaccgctaca
     3241 cgctggtcga gctcctgggc gacggcggca tggccgaggt
//
LOCUS       DBNGVD010000419         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1556683, whole genome shotgun sequence.
ACCESSION   DBNGVD010000419 DBNGVD010000000
VERSION     DBNGVD010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1556683"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1095)
                     /locus_tag="AC16CM_11280"
     CDS             complement(196..1095)
                     /locus_tag="AC16CM_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3140458.1"
                     /translation="MYSSERIALPAVFVGVLAVGAAAIFIRLAEAPALGIAFWRCALG
                     VVALTPLAAWRRERLPEGRDLWAGVAAGVALGVHFGTWIASLDYTSVAASVVLVSTTP
                     VFVAVAAYLLLGERTSVLSLGGIGLAILGTTVIAGGGSDTGNTAFLGNALALVGAVAM
                     AVYVLIGRSLRTGGVGALSYSIVGYSVAALALLPVALVSGAQLWGYSATTWFWLFVIT
                     LGPQLLGHTVLNWALEYVRASIVSGAVLAEPVIAALLAWLVLAEKPGQATLVGGVVVI
                     AGLYLLLRGDTTNPLPSGARDIG"
     gene            complement(1169..2098)
                     /gene="mdh"
                     /locus_tag="AC16CM_11285"
     CDS             complement(1169..2098)
                     /gene="mdh"
                     /locus_tag="AC16CM_11285"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3140459.1"
                     /translation="MAKSKVTVVGAGNVGGTTAQRLAERNYADVVLVDIVEGLPQGKA
                     LDILQSGPVLGFDSNVTGTNGYEDTAGSDVVVITSGSPRKPGMSRDDLLKTNQNIVGS
                     VTEEVVKHSPDAILVVVANPLDAMCHVALETSGFPRERVVGMAGILDTARYRTFIAQE
                     LGVSVRDVFALVLGGHGDTMVPLPSMATVGGVAITELLPEDRVEAIVDRTRNGGAEIV
                     GLLKSGSAFYAPSAAAVEMVDAILLDQKRVLPCAAHLQGEYGIEDLFVGVPVKLGASG
                     IEEIVELDLREDEKAALSNSADSVQELVQVLHG"
     gene            2222..>2664
                     /locus_tag="AC16CM_11290"
     CDS             2222..>2664
                     /locus_tag="AC16CM_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3140460.1"
                     /translation="MIHAIETRQSVNRVKQEPVPRELIERVLESAVHAPNHKITEPWR
                     FHVFTGRGRGGLARARAETARLSAEAEGEHGDMMAGRISRERKKAFRAPVVIAVISVA
                     GRDEVDGAMATWLTTDTESNELLDREPLALLIGMLLDQQVPIETA"
BASE COUNT          473 a          896 c          880 g          415 t
ORIGIN      
        1 ccgcttcagc gtcagcctcc acgactgcca cagcttccgc ctcggcctcc gccactgtgg
       61 agcagatgcc tggaacaggt gggcccgtgc tctccgttcc gctgcttctg gtggccgccc
      121 tgatggccgg cggggcgagc ctggtcgtcg cgaggctgat cgtcagccga aggtaggggt
      181 tccgggccag gggggctaac cgatatcccg ggccccgctt ggcaacgggt tcgtcgtgtc
      241 gccgcgtagc aagaggtaca ggccggcgat gacgactacg ccgcctacca gcgttgcctg
      301 gcccggtttc tccgcgagga ccagccacgc cagaagggcg gctattactg gctcagccag
      361 taccgccccc gacacgatgg aggcccgcac gtactccagc gcccagttca ggaccgtgtg
      421 gccgaggagt tgggggccaa gcgtgataac gaacagccag aaccacgtgg tggccgaata
      481 accccacaac tgcgcacccg agaccagggc gacggggaga agcgcgagtg ctgcgacgga
      541 gtagccgacg atggagtagg agagcgcacc aacgccgccc gtccggaggg aacgcccgat
      601 caggacgtag accgccatcg cgacggcccc aaccagggca agggcgttac cgaggaacgc
      661 cgtattgccc gtatcggacc ctcccccagc gatgaccgtc gtgcccagga tcgcaagccc
      721 gataccgccc agggatagga ccgacgttcg ctctccgagc agcaggtatg cggcgacggc
      781 cacgaagacc ggcgtcgtgc tcacgagcac gacgctcgcc gccacgctcg tgtagtccag
      841 ggaggcgatc caggtcccga agtggacccc cagggccacc cccgccgcca cgccggccca
      901 gaggtcacgt ccttctggca agcgttcccg cctccacgcg gccaggggcg tgagcgccac
      961 cacgccgagg gcgcaacgcc agaatgcgat gcccagcgcc ggggcttcgg ccaggcgaat
     1021 aaagatcgcc gccgcaccga cggcgagcac gccaacgaac acggcgggca gcgcgatccg
     1081 ctcgctagag tacaccgcct accgggttcg cctcgtcatc gcacgaaacg tggagaggac
     1141 gatggactac gtcagttgct gctgatgtct agccgtggag cacctgcacc agctcctgaa
     1201 cggagtcagc gctgttgctc agggcggcct tctcgtcctc cctgagatcc agctccacta
     1261 tctcctcgat accggaggcg ccgagcttga ctgggacgcc cacgaagaga tcctcgatgc
     1321 cgtactcgcc ctgcaggtgg gcggcgcacg ggagcacgcg cttctggtcg agcagtatgg
     1381 cgtcgaccat ctctaccgcc gcagcggacg gcgcgtagaa ggcgctgccg ctcttgagga
     1441 ggccgacgat ctcagccccg ccgttacggg tgcggtccac gatggcctcg acgcggtcct
     1501 cgggcaggag ctccgtgatt gccacgccac cgaccgtggc catgctgggc agcgggacca
     1561 tggtatcccc gtggccgcca aggacgagcg cgaacacgtc gcgcaccgaa accccgagtt
     1621 cctgggcgat aaaggtgcgg tagcgggccg tgtccaggat gccggccatg ccgacaaccc
     1681 tctcgcgggg gaagccgctc gtctcgagcg ccacgtggca catcgcgtcg agcgggttgg
     1741 cgaccaccac caggatggcg tcgggggagt gcttgacgac ctcctcggtt acgctaccca
     1801 cgatgttctg gttggtcttc agcagatcgt cgcggctcat gccgggcttg cgcgggctgc
     1861 cgctggtgat cacgaccacg tcagagccgg ctgtgtcctc atatccgttg gtacccgtca
     1921 cgttggagtc gaagcccagg accgggccgc tctgcaggat gtccagggcc ttgccctgcg
     1981 gcaagccctc gacgatgtcc acgagcacca cgtcggcata gttgcgctcg gcgagccgct
     2041 gcgcggtcgt gccacctacg ttgcccgcac cgacgaccgt taccttgctc ttcgccatat
     2101 ctttccctcc agtgttcgac gtatctatgt gccaatgata tgtgaattct tccgggcctg
     2161 ccgcgtcgag tccagctaga atcggtaggg caaacgatac tttcagggag cttttttgga
     2221 tgtgatccat gcaatagaga cgcgccagag cgtgaaccgc gtgaagcagg agcccgtgcc
     2281 gagggaactg atagagcgcg ttctggagag cgccgtgcac gctcccaacc acaagatcac
     2341 cgagccctgg cgtttccacg tctttaccgg ccgaggtcgt ggcgggctgg cgcgggccag
     2401 ggccgagacg gcgcggctct cggccgaggc cgaaggagag cacggggaca tgatggctgg
     2461 caggatcagc cgcgagcgaa agaaggcttt cagggccccg gtcgttatag ccgtgatctc
     2521 cgtcgccggc cgcgacgagg tagatggcgc catggcgacc tggctgacca cggacaccga
     2581 gtcgaacgag ctgctcgacc gcgagccgct ggcgctgctc atcggcatgc tgctggacca
     2641 gcaggtgccg atcgagaccg cgtt
//
LOCUS       DBNGVD010000420         3716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_829178, whole genome shotgun sequence.
ACCESSION   DBNGVD010000420 DBNGVD010000000
VERSION     DBNGVD010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_829178"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="AC16CM_11295"
     CDS             <1..942
                     /locus_tag="AC16CM_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3140461.1"
                     /translation="DVFPNDHPNFLGHLALGTPPEILGSLKDADLVLVIGCRLSEVTT
                     QSYSLPHGDQAVIQVDLDPDEVGATVPVEIGMVSDARSALTAFVARAQPDGAVRDWTE
                     AHGAYLKASTVPPSRSAGGVDPSQVVGAMLETLPADSLLTNDAGNFSVFLHRYWRFNH
                     PDTQLASTNGAMGYGVPAAVAAKLAAPERTVVACCGDGGFLMTGQEMETAVRYGAPIT
                     VVVFRNGLQGTIAMHQQRDLGRTAGVTIGEVDLAGYARSLGAEGHTVTDPEDLVPALR
                     DASSSDAVSLVDVVTDPDVISPSARLSDLAANTPRSN"
     gene            complement(939..1436)
                     /locus_tag="AC16CM_11300"
     CDS             complement(939..1436)
                     /locus_tag="AC16CM_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140462.1"
                     /translation="MDRGDTRSNLVVLRESRMWTQVRLAEEAGISPTTVSGIENGRIS
                     RPHFGTVRKLAVALEVEPEDLLPRLQRGGRPEGLSLEWSMASDEEEFDKEVEAASIEG
                     LSTLFEMLGDERLRLRKLYEEARGDRQRRLIKAMIRQVAANSGSVEASLMAHPDRDGA
                     RQGPG"
     gene            1614..2102
                     /locus_tag="AC16CM_11305"
     CDS             1614..2102
                     /locus_tag="AC16CM_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3140463.1"
                     /translation="MALSPFRGFYDMQSEMNRMFDEVFGDVSRGSGRQQGEAPSRWAP
                     ALDALQENGDIVVRAELPGVKPGDVDITLHNGVLTIAGERRAEERREGSGYYVRERRY
                     GSFRRSMTLPQGVDESSISARFDDGVLEVRVSGAAAVQEPRRIQIESGDEETTDNGSS
                     GS"
     gene            2185..2388
                     /locus_tag="AC16CM_11310"
     CDS             2185..2388
                     /locus_tag="AC16CM_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140464.1"
                     /translation="MEDIERRVARERARVESEYRRVLRTSVEFERRIRDRWREKSREK
                     NRRIAELEERVAELERCLTEKSP"
     gene            complement(2476..2769)
                     /locus_tag="AC16CM_11315"
     CDS             complement(2476..2769)
                     /locus_tag="AC16CM_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140465.1"
                     /translation="MADRQTRERKLVALMVNDLGPGFGEQEMAGLEQDVRSLPDEKLR
                     DTLVTRLDLPNSVDDETLEETLLHVEEPEGISGHDFTRAPDMSHEPRPEERRD"
     gene            complement(2826..>3716)
                     /locus_tag="AC16CM_11320"
     CDS             complement(2826..>3716)
                     /locus_tag="AC16CM_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR3140466.1"
                     /translation="AENVEAQLALYREDMNRDFEARMAEIENIVLRMNERGDAWLEEN
                     IRFGNVLELFRQEKVSERFEREVVADTEELVDERVDELVDWMVDRNLKQWRTVVDYVN
                     RRRQADYDERLIGEIGDTFEYNRGELLRSVGKNAQDVVQRYDRERESQKLALSLQGAV
                     AQTAAFEVGAVGIGAVVVAIATTRFLDVTGIIAGAILAGYGLFILPNRRRKAREEFRE
                     KTDALRERLGEVVRRQFESELGRSIERMREAIAPYTRFVRSEHARMTRAGEDLAAIDA
                     EADALRTEISAPGVGPMGKT"
BASE COUNT          649 a         1206 c         1201 g          660 t
ORIGIN      
        1 gatgtgttcc ccaacgatca cccgaacttc ctgggtcacc tcgcgctcgg cacgccgccg
       61 gagatcctgg gaagcctgaa agatgccgac ctcgtcctgg tcatcggctg ccgcttgagc
      121 gaggtgacga cccagagcta ctcgctcccg cacggggacc aggccgtgat ccaggtagac
      181 ctcgaccccg atgaagtggg ggcgacggta ccggtcgaga tcggcatggt ctccgacgcc
      241 cggtcggccc tcaccgcgtt cgtcgcccgg gcccagccgg acggtgcggt gcgggattgg
      301 accgaagccc acggagccta cctgaaggcc agcaccgtac ccccgtcccg tagcgcgggg
      361 ggcgtcgatc cctcgcaggt cgtaggagcc atgctggaga ccctgcccgc agactcgctg
      421 ctgacgaacg acgcaggaaa cttctcggtc ttcctccacc gctactggcg cttcaaccac
      481 ccggatacac agctcgcctc gaccaacggc gcgatgggct atggtgttcc cgcggcggtg
      541 gcggccaagc tcgcggcgcc cgaaaggacc gtggtcgcgt gctgcggcga cggcggtttc
      601 ctgatgaccg gccaggagat ggagacggcg gtcaggtacg gcgcccccat caccgtcgtg
      661 gtgtttcgaa acggattgca gggcaccata gccatgcacc agcaacgtga cctcgggcgc
      721 accgccggcg tcaccatagg cgaggtcgac ctggccggct acgccagaag cctcggcgcc
      781 gaaggccaca cggtaaccga ccccgaggac ctcgtcccag ccctgcggga cgcgtctagt
      841 tccgacgccg tcagcctcgt cgacgtggta acggacccgg acgtgatcag cccctccgcc
      901 aggctctcgg atctcgccgc gaatacaccc cgtagcaact agcccggtcc ctgccgagcg
      961 ccatccctgt cggggtgcgc catgagagag gcctccaccg accccgagtt ggctgcgacc
     1021 tgccggatca tggccttgat gagccgtcgc tgccggtctc ccctcgcctc ctcgtagagc
     1081 ttgcgcaggc gcaaccgctc gtcgccaagc atctcgaaga gcgtggagag cccctcgata
     1141 gaggcggcct ctacctcctt gtcgaactcc tcctcgtccg acgccataga ccactccaga
     1201 gagagccctt caggccgacc gccccgctgc aagcggggaa ggagatcctc cggctcgacc
     1261 tcgagggcca cagccaactt acgcaccgtc ccgaaatggg ggcgagagat cctgccgttc
     1321 tcgatccccg aaacggtagt cgggcttatc ccagcctcct ccgcgagccg aacctgcgtc
     1381 cacatgcgcg attccctcaa cacgacaagg ttgcttctcg tgtcgcctct gtccaccaac
     1441 cacctcttcg tccgaatacc tcctaacctt accatcgttg gaacgaaact gtagaaaaat
     1501 actagaatct gtccaaaaac tgtttacaaa ctgttgtaga gggatataat gtccgtgctg
     1561 aggtaggagt ggccctggtt cagggggtcg cagagaacga ggaggtaggc gagatggctc
     1621 taagtccttt ccgtggtttc tacgacatgc agagtgagat gaacaggatg ttcgacgagg
     1681 tgtttggcga cgttagccgc ggtagcgggc ggcagcaggg ggaggcgccc agtcgctggg
     1741 ccccggcgtt ggacgcgcta caggaaaacg gcgacatcgt ggtcagggcg gagttgcctg
     1801 gtgtgaagcc tggggacgtg gacatcacgt tgcacaacgg ggttctgacc atcgctggtg
     1861 agcgcagggc agaggagcgg cgcgagggtt ctgggtacta cgtgcgagag cgacgttacg
     1921 ggtctttccg caggagcatg acgttgccgc agggtgtaga cgagagcagc atcagcgccc
     1981 gtttcgacga cggcgttctg gaggtaaggg tttccggggc ggcggccgtg caggagccaa
     2041 ggcgcataca gatcgagtcg ggcgacgagg agactacgga taacggtagc tcgggaagct
     2101 gaacgtcacc ggcgactcgt cgggaggcac cggggctcta gcggcctcgg tgccttctca
     2161 tctgccggtt tacactggtc tcctatggag gatatcgagc ggcgggtagc acgggagcga
     2221 gccagggtag agtccgagta caggagggtc ctccggacct cggtcgagtt cgagcgcagg
     2281 atcagggacc gctggcggga gaagagccgg gagaaaaacc ggcgcattgc cgagctagaa
     2341 gagcgagtcg ccgagctgga gcggtgtctc acggagaaaa gcccgtaggg gcgggtttcg
     2401 aacccgcccg gaaatcaacg ccgtctacgg gctactcctc caggtgtaac gagggcaggt
     2461 agtagcgccg ggcggctagt ccctccgctc ttccgggcgt ggttcgtggc tcatatctgg
     2521 tgcgcgggtg aaatcgtggc cggagatccc ttccggttct tcgacgtgaa ggagggtttc
     2581 ctccagggtc tcatcgtcga cggagttggg gagatccaac ctcgtgacca gcgtgtcacg
     2641 gagcttttcg tctggtaggg agcgcacgtc ctgctcgagc ccggccatct cctgttcccc
     2701 gaaaccggga cccagatcgt tgaccataag tgctacgagc ttgcgttcac gcgtctggcg
     2761 atccgccatc tatgcctcct cgaacgtctc tggcatccga gtctacaacg gaagcgggac
     2821 cgtgttcagg ttttgcccat cggcccgacg ccgggggcgg agatctccgt ccgcagggcg
     2881 tccgcctcgg cgtcgatggc ggcgaggtct tcgccggccc gggtcatgcg ggcgtgctcg
     2941 gaccggacga agcgggtgta gggggcaatt gcctccctca tccgctcgat ggagcggccg
     3001 agctcagact cgaattgccg tctgaccacc tcgcctaggc gctcgcgcag ggcgtcggtc
     3061 ttctcgcgga actcctcgcg ggccttgcgg cgacgattgg ggaggatgaa cagcccgtag
     3121 ccggccagta ttgccccggc aatgatcccg gtcacgtcca ggaagcgggt ggtggcgatg
     3181 gcgaccacga cggccccgat gcccacggcc cctacctcga aggccgctgt ctgggcgacg
     3241 gcgccctgca aggagagcgc cagcttctgg gactcgcgct cgcggtcgta acgctgcacc
     3301 acgtcctggg cgttcttgcc gaccgagcgc aggagctcgc cacggttgta ctcgaaggtg
     3361 tcgccgatct ccccgatcag acgctcgtcg tagtccgcct ggcgccgcct gttcacgtag
     3421 tctacgaccg tgcgccactg tttgaggttc ctgtctacca tccagtccac gagctcgtct
     3481 acccgctcgt ccacgagctc ctcggtatcg gcgacgacct cccgctcgaa gcgctcgcta
     3541 accttctcct ggcggaacag ctcgaggacg ttgccgaagc ggatgttctc ctccagccag
     3601 gcgtcgcccc gttcgttcat cctcaggact atgttctcga tctcggccat cctggcctcg
     3661 aaatcccgat tcatgtcctc cctgtagagc gcgagctggg cctccacgtt ctccgc
//
LOCUS       DBNGVD010000421         6589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4836301, whole genome shotgun sequence.
ACCESSION   DBNGVD010000421 DBNGVD010000000
VERSION     DBNGVD010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4836301"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..713
                     /locus_tag="AC16CM_11325"
     CDS             327..713
                     /locus_tag="AC16CM_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014233559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR3140467.1"
                     /translation="MSWYLEALKKYAVFSGRARRMEYWYFVLFNVIVAFVLALIDALL
                     GTTTGVSSFGLLSGLYSLAVLIPTLAVLVRRLHDIDRTGWWILINLIPLVGTIVLLVF
                     ALMPGTPGSNQYGPDPKQATGEARVV"
     gene            complement(718..1863)
                     /locus_tag="AC16CM_11330"
     CDS             complement(718..1863)
                     /locus_tag="AC16CM_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3140468.1"
                     /translation="MTEPLDLTSVPVVDNHCHGILLDQDFGDTAGWRGAFTESTEPGM
                     VRDHVATTAFYGRLIRTLAGFLDCEPEEQAVFAARTGRDGLGLAAELLRAANIDTLLV
                     DTGFPPPSDVLPVPEFGDLGGCGAEPMLRLEVLMEDLLEQNDSLGGTEEALDAALDDV
                     RARGYVALKSIAAYRTGLDIQEWTRDEAEDSFRQYRRDAEKGSARLVHKPLLDTLSHV
                     AFAQAARQEVPVQFHVGYGDADTDLLLGNPLYLRGVLRRPDYRGMPVVLLHECYPYTR
                     QGGYLAAVYENAYLDLSYGIPLLGYGEMLSFTRAAIGVAPTSKLMYSSDGIGVPELHW
                     ISALDGRRVLGEVLGEMASHGELALHEAESAGEAILRTNARRLYGLG"
     gene            complement(1959..3236)
                     /locus_tag="AC16CM_11335"
     CDS             complement(1959..3236)
                     /locus_tag="AC16CM_11335"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3140469.1"
                     /translation="MRFTFVDHAGIPKAKAVYRDGFAGRARSGVGLAKGVLALDPSGA
                     LNLESGLSAVGECRLIPDLSTLTPLPFARGQAMVACDMTEPDATTPWPGCPRSALKRM
                     LGRLSERGYRTVASFEAEFYVWGPEGPLDMTPYATSWALTPAAELAAELAEALREMGI
                     RPEQFHAEVGQGNLELSVGEDEALTAADKRVMVLEAIRGVAHRLGLETTMAPKPYLEG
                     AGNGHHLHVSLYEDESSVLFDETGALSRAGAGFVAGILEHLPAVMAFTAPSPNSYQRL
                     TPGMWSSDFGAYGVDNREAAVRLASPVAGRESATANVELKPADVTANPYLALAAVLAA
                     GMDGMDRGLDPGEPTMVDPATLSEEERASRGIRRLPASPDEALDALERDETLKAALGE
                     PLVRTHIAVARAQAAIARELSPEEIAATAATLY"
     gene            complement(3370..4866)
                     /locus_tag="AC16CM_11340"
     CDS             complement(3370..4866)
                     /locus_tag="AC16CM_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3140470.1"
                     /translation="MSTEARNAGGADSPHLRRELRLWEAVGISLALMAPSMAANINPQ
                     GTAGSVGRAVPLAFALATIGVLLIAWTFVRLTQRFDHAGSVYGFVGATLGPRTGVISG
                     WALMGTYTFYGVVTATAAGIFGAEFLDALGIWTDQPGWAPFVVGAIALAGVWALAASN
                     IRGGTRVLLVIEGTTVALILVVSVIILLRLATDTAPNGNTLDLSVFTVPGGTGLSAVF
                     LGVVFGFLSFAGFEAAATLGEEAREPRRDIPRAILWVAVFGGLYFIFVTAVEVMGFGT
                     DAKGTRAFINSGSLLGDLGSQFVAGWVGELITIGAAVSAFGCALACVVGATRLLYALS
                     RDEVGPAPLGTVSPGSGVPARSTATVALGAYVIIALGWFVFGVAPFDLFVASGTIGTL
                     ILLLVYALATIGAAKLLFFSGERLVAAWEVIVPFLALVLIGYTLFRNVYPYPTDAAAW
                     YPIIAALWVMVGIAVTFVRNAATRRAGERLMEEEGLAAESDSRTGGYG"
     gene            complement(5024..6430)
                     /locus_tag="AC16CM_11345"
     CDS             complement(5024..6430)
                     /locus_tag="AC16CM_11345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3140471.1"
                     /translation="METVIQGSTLETLRAGLRGTAYAPGEEGYDAASQAWNLAAHQQP
                     ALVVVAGGAADVIAAVRFARDAGMGVGVMATGHGVGMPCDGGVLINTSGMRAVRVDPV
                     ARTARVEAGALWKDLVHEAAPHGLAGLMGSTSGVGIVGYTMGGGFGWLGRKYGFNAAS
                     MRQADVVTADGELVRVGADEHHELVWGLGGGGGNFGIVTSLEFDLYPVGTLYAGNLIY
                     PMEKAAEVLEAYADWSADLSDEWSTGVAFLNVPPLPAVPEPLRGKSVIALRGCYCGGS
                     PDDGEEQLRPVREKLGETIMDTFGPMPFAAMDAISMDPVDPMGARQHSEMLADLTPEA
                     IETLVEVVGAGSESPLIVLELRQLGGALQRTADRLSTMGAGESRFIMNGVGPAFTPEM
                     AEGVVAYLKGVAEATRPFQTGDTYVNFMELDGASPERVRASYAPEDYERLVALKDSYD
                     PNNLFRFNRNIPPSQNGS"
BASE COUNT         1094 a         2218 c         2160 g         1117 t
ORIGIN      
        1 cagaaggagc agcagaccta gcagccgcgt ctgtctgtac aatgctcgcc ctccggatcg
       61 agtttcgaac ctctcgtcac cccgacagga aggataaccc ttcctattga cccgcgcatc
      121 ttcccgcgcg gtatacgggg agaagctact tcttgcggga ccataccagt aggcgctccg
      181 gcccgccgac catgctccac catgatagcc tcgagactac ggtagcgtgg agcaattcct
      241 cttaccgtgt cttgtttcgc acaagtagtt cactaactat gctaatgtgt atggtgggtg
      301 tagatagcta gacggggagg ttcggggtga gttggtatct ggaagcgctc aagaagtatg
      361 ccgtgttcag cggcagggca aggcgcatgg aatactggta cttcgtgctt ttcaacgtca
      421 tcgtggcttt cgtgctggcg ctgatcgacg ctttgctcgg caccaccacc ggagtttcga
      481 gctttggcct tctaagcggc ctctacagcc tggcggtgct catccccacc ctggcggtgc
      541 tggtgaggcg gctgcacgac atagaccgca cgggatggtg gatcctcata aacctgatac
      601 cgctggtcgg cacgatcgtg ctgctggtct tcgcgctgat gccagggacg ccaggcagca
      661 accagtacgg tcccgatccg aagcaggcca cgggcgaggc cagggtagtt tgagggccta
      721 gccgagcccg tacagacgcc tggcgttcgt acgcaggata gcctctccgg ctgattcggc
      781 ctcgtggagg gcgagctctc cgtgggacgc catctcgccg aggacttccc ccagcacgcg
      841 ccgtccgtcg agggcggaga tccagtgcag ttcgggcacg cctatgccgt cggagctgta
      901 catgagcttg gaggtcgggg cgacgccgat cgcggcccgc gtgaaggaga gcatctcgcc
      961 gtagccgagc agcggtatgc cgtaggagag atccaggtac gcgttctcgt agacggcggc
     1021 caggtagcct ccctgcctgg tgtacggata gcactcgtgc aacaacacga cgggcatgcc
     1081 acgatagtcg ggccgccgta gcaccccacg caggtagagg gggttgccga gcagaaggtc
     1141 ggtgtccgcg tcgccgtagc cgacgtggaa ctgtacgggg acctcctgcc gcgccgcctg
     1201 agcgaacgcc acgtgcgata gcgtgtcgag caggggtttg tgaacgagcc gcgcggagcc
     1261 cttttctgcg tccctcctat actgccggaa cgagtcctcc gcctcgtccc gcgtccattc
     1321 ctgtatgtcc aggcccgtcc ggtaggcggc tatgctcttg agcgccacgt agcccctggc
     1381 gcgcacgtca tccagggcgg cgtcgagcgc ctcctcggtc ccgcccaggg agtcgttctg
     1441 ctcgagcaga tcctccatca ggacctccag tcgcagcatg ggctccgcgc cgcagcctcc
     1501 cagatccccg aactccggta ccggcaacac gtccgagggc ggcgggaagc ccgtgtcgac
     1561 gagcagggta tctatgttcg cggcccggag cagctcggct gccaacccaa gcccgtccct
     1621 cccggtgcgg gccgcgaaca cggcctgctc ctccggctcg cagtcgagga agccggccag
     1681 cgttcttatg aggcgcccgt agaaggccgt ggtcgcgacg tgatcccgca ccatcccggg
     1741 ttccgtggat tcggtgaaag ccccccgcca gccagccgtg tctccgaaat cctggtccag
     1801 caagatgccg tggcaatgat tgtccaccac gggaacagac gtcaggtcta gcggttccgt
     1861 caccttgtct ccttttttga ggcttcaggt ttcagttttc aaccaggagc gtcgaaccgc
     1921 gaaaagctga cggctgaccg ctaatggctg atagctctct agtagagcgt tgcggctgtg
     1981 gctgctatct cctccgggga gagttctctg gctattgcgg cctgggctcg ggctacggct
     2041 atgtgggtgc gtaccagtgg ttctccgagg gccgccttta gggtttcgtc tctctccagg
     2101 gcgtccaggg cttcgtctgg ggaggcgggg agcctccgga tgccgcggga ggcgcgttct
     2161 tcctcgctca gggtggcggg gtctaccatg gtgggctcgc cggggtccag gccgcggtcc
     2221 atgccgtcca tcccggcggc gagcacggcg gcaagggcca ggtaggggtt ggccgtgacg
     2281 tcggccggct tcagctcgac gttggctgtg gccgattccc tgccggccac gggggacgcc
     2341 agcctcacgg cggcttccct gttgtccacg ccgtaggcgc cgaagtccga ggaccacatg
     2401 cctggtgtca ggcgctggta ggagtttggt gagggagccg tgaaggccat gacggccggg
     2461 aggtgttcca gtatgccggc cacgaacccg gctccggccc tgctcagggc gcccgtctcg
     2521 tcgaagagca cggaagattc gtcctcgtag aggctcacgt gcaggtggtg gccgttgccg
     2581 gccccctcaa ggtatggttt cggggccatc gtggtctcga ggcccagcct gtgggctacg
     2641 ccccggatcg cctcgagcac cattacgcgt ttgtccgccg ccgtcagggc ctcgtcctcc
     2701 ccaacggaga gctccaggtt gccttgcccc acctcggcgt ggaactgttc cgggcgaatg
     2761 cccatctccc gaagggcctc ggccagctcc gcggccaact ccgccgccgg ggtcagggcc
     2821 cagctcgtcg cgtacggcgt catgtccagc ggaccttccg gaccccagac gtagaactcg
     2881 gcctcgaagg aggcgaccgt cctgtagccc ctctccgata gccgtccgag catccgcttc
     2941 agggcgctgc gcgggcatcc tggccagggc gtggtcgcgt cgggctcggt catatcgcag
     3001 gcgaccatcg cctgcccgcg ggcgaagggc aggggggtca gcgtagagag gtcggggatc
     3061 aggcggcact cccccaccgc gctcagcccc gactcgaggt tgagagcccc gctcgggtcg
     3121 agcgccagga cccctttcgc gaggcccacc ccggacctcg ccctgccggc gaagccgtcc
     3181 ctgtagacgg cttttgcctt cgggatgccc gcatgatcca cgaacgtgaa cctcacccac
     3241 aaggtatcct ggggtaaagc tctgatctcc tcttcttgca tcgcaccccc tgcggctctg
     3301 tcgcacgtcc tgtccacgat ggcgcgccca atatctcacg ccctggctct tcggtccacg
     3361 gcgtcgtgcc tagccgtagc ccccggtcct ggagtccgac tcagcagcca ggccctcttc
     3421 ctccatcaac cgctcgccgg cccgccgcgt tgcggcgttt cggacgaacg tgaccgctat
     3481 tcccaccatg acccagagag cggcgatgat cgggtaccac gcagcggcgt ccgtcgggta
     3541 ggggtagacg ttccggaaca gcgtgtagcc gatcagcacc agcgccagga agggcactat
     3601 tacctcccag gcggcgacca gccgctcgcc cgagaaaaac agcagcttcg ctgcccctat
     3661 ggtggccagg gcgtacacga ggagcaggat cagggtgcct atcgttccgg aggcgacgaa
     3721 gaggtcgaag ggcgctacgc cgaacacgaa ccagccgagg gcgatgatga cgtaggcgcc
     3781 aagggcgacg gtggccgtgg agcgcgcggg gacgccgctt ccgggggata cggtgccgag
     3841 gggagctggt ccgacctcgt cgcgcgagag cgcgtacagc aggcgggtgg cgccaaccac
     3901 gcacgccagc gcgcagccga aggcacttac ggccgcacct atcgtgatca gctctcccac
     3961 ccaaccggcg acgaactggc tgccgaggtc acccaacagc gagcccgagt ttatgaatgc
     4021 ccgggtgccc ttcgcgtcgg tcccgaaacc catgacctcg acggcggtga cgaagatgaa
     4081 gtacaggccg ccgaagacgg cgacccagag gatcgcgcgc ggtatgtcgc ggcgcggctc
     4141 ccgcgcctcc tcacccaggg tggcggcagc ctcgaagccg gcgaaggaga ggaacccgaa
     4201 gacaaccccg aggaacaccg cagagaggcc agtcccgccc ggtaccgtga acaccgagag
     4261 atcaagggtg tttccgttgg gggcggtgtc cgtggccagc ctgagcagga tgatcacgct
     4321 gaccacgagg atgagcgcga ccgtggtgcc ctcgatcacc aggagtaccc tggtgcctcc
     4381 cctgatattc gaggccgcga gcgcccacac cccggccagc gcgatggcgc cgaccacgaa
     4441 cggcgcccag cccggctggt ccgtccagat gccaagggcg tcgagaaact cggccccgaa
     4501 gatgccggcc gcggtggcgg ttacgacgcc gtagaaggtg taggtgccca tgagggccca
     4561 acccgagatc acaccggtcc gcgggccgag tgtggccccg acgaagccgt acacgctgcc
     4621 ggcgtggtcg aagcgctggg tgagccttac gaaggtccag gcgatgagta gcacgccgat
     4681 ggtcgccagc gcgaacgcga gcggcacggc gcggcccacg ctgcccgccg tcccctgagg
     4741 gttgatgttg gccgccatgg agggcgccat gagcgccagc gagatgccga ccgcctccca
     4801 caggcgtagc tcgcgccgca ggtgcgggct atcagcgccg ccggcgttcc tggcttccgt
     4861 gctcatgggg acccccctgc ccggtagtgg ggaggaggta gtcgagggtt ggcccagatt
     4921 ccccttccca cgaggatgcc gacaatatct cacgccgggg agcctcggtc catagcaacg
     4981 ggggccgcac gaggcggccc ccaatctccg tctggaggtt gtgctagctt ccgttctgcg
     5041 agggtgggat gttgcggttg aagcggaaga ggttgttcgg gtcgtagctg tccttcaggg
     5101 cgacgaggcg ctcgtagtcc tccggcgcgt aggaagccct taccctctcg gggctggcgc
     5161 cgtcgagctc catgaagttg acgtaagtgt cgccggtctg gaaggggcgg gtggcctcag
     5221 ccaccccttt caggtaggcc acgacgccct ccgccatctc gggcgtgaag gccggtccca
     5281 ccccgttcat tatgaaacgg gattcgcccg cgcccatggt gctcaggcgg tcggcggtgc
     5341 gttgtagggc gccgccgagc tgacgaagct cgagcacgat gaggggcgat tcggaaccag
     5401 cgccgactac ctcaacgagc gtctctatcg cttcgggggt gaggtcggcc agcatctcgg
     5461 agtgctggcg ggcgcccatg gggtcgacgg ggtccatgct tatggcatcc atggccgcga
     5521 acggcatcgg gccgaaggtg tccatgatcg tctcgccgag cttctcccgc acgggacgca
     5581 actgctcttc gccgtcgtcg ggcgacccgc cacagtagca gccacgcagc gctatcaccg
     5641 acttgcctcg cagcggctcg gggacggcgg gcagcggtgg aacgttgagg aacgccacgc
     5701 ccgtcgacca ctcgtccgaa aggtcggcgc tccagtcggc gtaggcctcc agcacctcgg
     5761 ccgccttctc catagggtag atcaggtttc cagcgtagag ggttccaacg gggtagaggt
     5821 cgaactccag ggaggtgact atcccgaagt tgcctccccc gccgccgagg ccccagacaa
     5881 gctcgtgatg ctcatcggcg ccgacacgaa cgagctcacc gtcggcggtg accacgtcgg
     5941 cctgccgcat gctcgcggcg ttgaagccgt acttgcggcc cagccagccg aagccgcccc
     6001 ccatcgtgta cccgacgatc cccaccccgg aggtcgagcc catcaggccg gccagaccgt
     6061 ggggtgcggc ctcgtggacc agatccttcc acaacgcccc tgcttccacc ctcgccgtcc
     6121 ttgccaccgg gtctaccctg acggccctca tgcccgaggt gttgatcagg accccaccgt
     6181 cgcagggcat gccgaccccg tgcccggtcg ccatgacccc gacgcccata cccgcgtcac
     6241 gggcgaagcg cacagcggct atcacgtcgg cggcaccccc agcaacgacc acgagcgcgg
     6301 gctgctggtg ggcggccagg ttccaggcct ggctcgccgc gtcgtagccc tcctcaccgg
     6361 gcgcgtacgc cgtgccacgc aggccagccc gcaaggtttc tagggtagaa ccctggatca
     6421 ccgtctccac aatcgttctc ctttccagaa gagataggcc agcagaggcc aacgtctcca
     6481 agctcatcga ggcaactccc acagactaac ccccggtatc agcgggacac atcccccaaa
     6541 tggggtattt ttgagctgtc agctgtcagc tatcagccgt cagcttttt
//
LOCUS       DBNGVD010000422         8268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2104490, whole genome shotgun sequence.
ACCESSION   DBNGVD010000422 DBNGVD010000000
VERSION     DBNGVD010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2104490"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16CM_11350"
     CDS             <1..265
                     /locus_tag="AC16CM_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140472.1"
                     /translation="FFKITVPLAFPALVAGAVTAWARALGEFGATIIFAGNFRGITQT
                     IPLAIYSEFQSNIDAAVALSVLVLGFAFAVILTVRYLTGKMSR"
     gene            complement(316..534)
                     /locus_tag="AC16CM_11355"
     CDS             complement(316..534)
                     /locus_tag="AC16CM_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3140473.1"
                     /translation="MRLSTRNRLPGTIVDIERGKAATRVSLQVGDNHVVALITTESAE
                     ELGLEIGQQVTALVKATDVMVLVDEGVG"
     gene            complement(531..839)
                     /locus_tag="AC16CM_11360"
     CDS             complement(531..839)
                     /locus_tag="AC16CM_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR3140474.1"
                     /translation="MDLEPMFEELARSPGLGPFRGLIGRVEAYDLEHNSDLLRTLRVY
                     FAANTNTSEAADRLYLHRNSLTYRLARVEHLTGLDLKDHRARLALQLGLLAIRKEHQR
                     "
     gene            1218..1682
                     /locus_tag="AC16CM_11365"
     CDS             1218..1682
                     /locus_tag="AC16CM_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3140475.1"
                     /translation="MREVRVKVNGRDYVREVEPRMLLVHFLREELNLTGTMVGCNTSN
                     CGACTVLMDGESVKSCTMFAVQADGREIQNVQGLAENGSWHPMQTAFHEQHGLQCGYC
                     TPGMIMASVSLLKENPNPSEEEIREGLEGNYCRCTGYENIIKAVQQAAGQTG"
     gene            1696..4074
                     /locus_tag="AC16CM_11370"
     CDS             1696..4074
                     /locus_tag="AC16CM_11370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3140476.1"
                     /translation="MGVTEAPEKYVGGGVPRKEDPALVTGRANWTDNIKLPGMLHMAV
                     LRSPFAHARVTNLDVSAALEQPGVVAAFTADDLSDDWVAPMPCAWPVTEDIKMPDHWP
                     LAKGEVNFMGDAVAVVIADGRYTAQDALEFIEVDYEPLDVVVDMEEAIKDDAPLVHED
                     LGTNSSYVWPLEVGDVDGAFEQADVVIKERYIQQRLIPNAIEPRAVVANPDPTNDGIT
                     LYTATQVPHIVRLMISAVTGIPDQRVRAVAPDVGGGFGSKLNIYAEEILATNIARRLG
                     VPIKWVEDRSENYMATIHGRDQIQDIELAATSDGRILGMRTNLLADMGAYMQLVTPGI
                     PVLGAFMYCGVYQFDAYSFTCTGVFTNKTPTDAYRGAGRPEAAYAIERIVDSLARELD
                     MDPVEVRRKNFYEPFDEPTTTPAGIQYDSLNLPIVLDRVLEMADYDALREEQKRRREE
                     NDPVQIGIGLSTYTEICGLAPSQVLASLRYGAPGWESATVRMFGSGKVEVVTGTSPHG
                     QGHVTSWSQIAADALGVTPDDVEVLHGDTATSPHGLDTYGSRSLPVGGVAVHLAAQKV
                     QDKAKKIAAHMLEASEDDLEFEGGQFSVAGSPDQSVTIQDVAAAAFGGINIPEDMEPG
                     LTADTFYDPPNFVFPFGAHICVVEVDTETGKTGVRDYFGVDDCGPVINPIIVDGQVHG
                     GIAQGIAQALYEEAIYDEDGTLVTGSMVDYMIPGAPELPNYTLDRTVTPSPTNVMGVK
                     GIGEAGTIGSPPAVINAVIDALSPFGVTHIDMPASPMRVWDAIQNARDGGEA"
     gene            4074..4925
                     /locus_tag="AC16CM_11375"
     CDS             4074..4925
                     /locus_tag="AC16CM_11375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3140477.1"
                     /translation="MYPMPFDYEVAESVDQAIELLGEHGDESKLIAGGHSLIPLMRLR
                     LAAPTVLIDLDRLDDLRYVSDEGDHLAIGALTRHREVLNNPLVQEHCGILSFTAGLLG
                     DPSVQHRGTIGGALAHGDPSGDLPTVVKTLEGELVIKGPDGERTIQAADFFQDYLMTD
                     LDEREVITEVRVPKLSPNTGWAYKKFSRRSQDWAIVGVAAVVEKNNGTIGSARIGLTS
                     MGSTPIRASAVEEALSGASPDGVAEASEAADEGTEPASDGAASADFRRHLARVWTRRA
                     VEEALSR"
     gene            4922..5854
                     /locus_tag="AC16CM_11380"
     CDS             4922..5854
                     /locus_tag="AC16CM_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3140478.1"
                     /translation="MNDAANGRRGALPPGVSRSSELSARLREGGYLADEGLSTAVFLA
                     LKLGRPLFLEGEAGVGKTEVAKVLSRVLDTPLIRLQCYEGIDVNQAVYEWDYARQLLH
                     IRTAEALGMAEDRASFEDELYTRQFLVSRPLLRALEHHGPMPPVLLIDEIDRADDEFE
                     AFLLEILSEFAVTIPELGTIRADRPPVAVLTSNRTREVHDALKRRCLYFYIDHPGLER
                     EVEIVRLRVPGAEEQLARQVAQAVQNLRVMDLYKPPGIAETLDWTEALVALGARELDE
                     DLISATLGSVLKYREDQQRTMGMALSLLAGGGNA"
     gene            5847..6998
                     /locus_tag="AC16CM_11385"
     CDS             5847..6998
                     /locus_tag="AC16CM_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3140479.1"
                     /translation="MPDTATPETGPPLMLDAAVTFGRVLRRMGLPAGPDRVVEFVRAV
                     EELDVTRREDVYWAGRITMCSNPDHLELYDRAFRLFWDGEEPPRIVPPPKSSFSASAA
                     EDSVQPPKKTVEKSERGEEAVALRYSPVDVLRKRNFADCTPEEFAELYRLMADLRLAG
                     DTRKGRRLGPAPRGRRDARRTLRDALRTGGEPLRHRYRKARDQPRRIVLLCDVSGSMA
                     SYSRTLLRFMHASVVSGEPVEAFVLGTRLTRVTRELADRDPDRAIGEASRAVEDWSGG
                     TRLGDTIKEFVDRWGQRGMARGAVVVILSDGWDRGDVSVLAESMERIARLAHRVVWVN
                     PLKAAPGYRPLARGMAAALPHVDVFLSGHNFESLEELARAISGVAEGER"
     gene            7146..7460
                     /locus_tag="AC16CM_11390"
     CDS             7146..7460
                     /locus_tag="AC16CM_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3140480.1"
                     /translation="MNPVVDKVASWREEGKQAALATVVKVERSGPLGPGTSMAVSEDG
                     QVAGSVSGGCVEPAVFEEAMAAIQSGEPKYLTYGISDDEAVGVGLTCGGIIHIFVERV
                     DW"
     gene            7450..>8268
                     /locus_tag="AC16CM_11395"
     CDS             7450..>8268
                     /locus_tag="AC16CM_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3140481.1"
                     /translation="MTGSGMGELLRRWHEALADQSPVAMATVIQGPGTGNKLLVEPVG
                     HVGTAGNDELDKAVVEAARGLLEGGRTETRHFGPRGQRRMEDVAVFIQSFAPPPRMYV
                     FGAIDFASALARLGKFMGYRVTVCDARAVFATRERFPSVDEVVVAWPDEFLETAQVDK
                     RTVICVLTHDPKFDVPVLKAALATEAGYIGAMGSRRTHDNRTARLKDEGATEEDLRRV
                     SSPIGLDIGARTPEETAVAIAAEIIALRTGHSGGRLAERSGAIHAPEEEGATARN"
BASE COUNT         1467 a         2684 c         2792 g         1325 t
ORIGIN      
        1 gttcttcaag atcaccgtac cgctggcgtt cccggcgctt gtcgccgggg cggtgacggc
       61 gtgggcgcgg gcgctcggag agttcggggc aacaataatc ttcgcgggaa acttccgcgg
      121 tatcacccag accatacccc tcgccatcta ctcggagttt cagagcaaca tagatgccgc
      181 cgtggcactc tcggtcctgg tactgggctt cgccttcgcc gtgatcctga ccgtcaggta
      241 cctcactggt aagatgtcca gatgagcggt tcgcgcagcg aaccgcgctg tcagccttca
      301 gctatcagcc gtcagctatc caacaccttc gtctaccagc accatcacgt cggtcgcctt
      361 gactagcgcc gtgacctgct ggcctatttc caggccgagt tcttccgcgc tctctgtagt
      421 gatcagggcc actacgtggt tgtctccgac ctggagcgag accctcgttg cggccttgcc
      481 tctctctata tccacgatgg tccctgggag ccgattgcgg gtgctcaacc tcacctctga
      541 tgctcctttc gaatcgccag caggccgagc tgcagcgcca gcctcgcccg gtggtccttc
      601 aagtctagcc cggtgagatg ctcaacgcgc gcgaggcggt aggtgaggct gttgcggtgc
      661 aggtagaggc ggtccgcggc ctcgctggtg ttggtgttgg cggcgaagta gacgcgcagg
      721 gtgcggagga ggtcgctgtt atgctcgagg tcgtaggcct ccacgcgccc gatcaggccc
      781 ctgaacggtc ccagcccagg cgaacgcgcc agctcttcga acatcggttc cagatccaag
      841 aaccctccag gcgcagagat ctactccccc gaccacgcgc agattctaca ggcgcggcgg
      901 ggatcgtaca gaccggaaga ggccggatca catgaccctc cccacgtccc ttttctttgc
      961 taaagattat tattacaata tgagcacttt gcagaaaacc ggcaaagtgc acagaaaaat
     1021 ctgccgtgat acactgcccg cactttcgag cagaaagggg tgtcaggggg acgcgatcgc
     1081 catacttaag gggaaaagcg agtctacgtg acagctttga cggcggtaca ggtatcgccg
     1141 gggggaacat gggtgtcggt gtggtttgca ctgacgcgta ggctggtcgg gggacgcgta
     1201 acggaaagga gagagatttg cgagaagtac gggtcaaggt aaacggccgc gattacgtgc
     1261 gcgaggtgga gcccaggatg ctgctggtcc atttcctgcg cgaagagttg aacctgacgg
     1321 ggaccatggt gggatgcaac acgtccaact gcggggcctg cacggtcctc atggacggcg
     1381 agtctgtcaa gtcgtgcacg atgttcgcgg tccaggccga cgggcgcgag atccagaacg
     1441 tccaggggct cgccgagaac ggcagttggc atcccatgca gacggctttt cacgagcagc
     1501 atggtttgca gtgcgggtat tgcacgcctg gcatgatcat ggcatccgtg agcctgttga
     1561 aggagaaccc gaacccctcg gaggaggaga tcagggaggg tctcgaaggc aattactgtc
     1621 gctgcacggg ctacgagaac atcatcaagg ccgtccagca ggctgccgga cagacgggct
     1681 aagggaaaga gaggcatcgg cgtgaccgaa gcaccggaga agtacgtcgg gggaggggta
     1741 ccccgcaagg aggacccggc cctcgtaacc ggaagagcca actggaccga taacatcaag
     1801 ctaccgggga tgctgcacat ggccgtgttg cgcagcccct tcgcgcacgc cagggtgacc
     1861 aacctggacg tctcggcggc cctcgagcag cccggcgtcg tcgcggcttt cacggccgat
     1921 gacctttcgg acgattgggt ggcgcccatg ccctgcgcgt ggcccgtaac cgaggatatc
     1981 aagatgcccg accactggcc gctggccaag ggtgaggtca acttcatggg ggacgccgtc
     2041 gctgtggtaa tagccgacgg caggtacacg gcccaggacg ccctcgagtt tatcgaggtg
     2101 gactacgagc cgctcgacgt ggtcgtggac atggaggagg cgatcaagga cgacgccccc
     2161 ctggtgcacg aggacctggg gaccaactct tcgtacgtgt ggccgctcga agtcggcgac
     2221 gtggacggcg cgttcgagca ggccgacgtg gtgatcaagg agcgctacat ccagcagcgt
     2281 ctcatcccga acgccatcga gccccgggcc gtggtcgcca accccgaccc gaccaacgac
     2341 ggcattacgc tctacaccgc cacccaggtt ccgcacatcg tcaggttgat gatctctgcg
     2401 gtcaccggaa taccggacca gagggtcagg gccgtggcgc cggatgtggg gggtggtttc
     2461 ggctccaagc tgaacatcta cgccgaagag atcctggcca ccaacatcgc caggaggctc
     2521 ggggttccga tcaagtgggt cgaggaccgt tccgagaact acatggcgac catccacggc
     2581 cgcgaccaga tccaggacat cgagctcgcc gccacgagcg acggcaggat ccttggcatg
     2641 aggaccaacc tgctggccga catgggcgct tacatgcagc tggtcacgcc aggcatcccg
     2701 gtgctcggcg ccttcatgta ctgtggcgtc taccagttcg acgcctattc cttcacctgc
     2761 accggcgtct tcaccaacaa gaccccgacg gacgcctacc ggggggctgg ccggcccgag
     2821 gccgcctacg ccatcgagcg catcgtggat tcgctggcgc gggagcttga tatggacccg
     2881 gtggaggtca ggcgcaagaa tttctacgag cccttcgacg agccgaccac gacgccggcc
     2941 ggcatccagt acgactcgct gaacctgccc atagtgctcg acagggtgct ggagatggcg
     3001 gattacgacg ccttgaggga ggagcagaag cgcaggcggg aggagaacga ccccgtccag
     3061 atcggcatcg ggctctcgac gtacaccgag atctgcggcc tcgccccttc ccaggtgctc
     3121 gcctcgctgc gctacggtgc ccctggctgg gagtctgcga cggtcaggat gttcggctcg
     3181 ggcaaggtcg aggttgttac gggcacctcg ccgcacggcc agggccacgt aacctcgtgg
     3241 tcccagatcg ccgccgacgc cctcggcgtg acccctgatg acgtggaggt cctccacggc
     3301 gacacggcga cctccccgca cgggctcgac acctacggtt cgaggagttt gcccgtcggc
     3361 ggcgtcgccg tccacctggc tgcccagaaa gtccaggaca aggccaagaa gatcgccgcg
     3421 cacatgctcg aggcctccga ggacgacctg gagttcgagg gtggccagtt cagcgtggcg
     3481 ggctcgcccg accagagcgt caccatccag gacgtggccg ccgcggcctt cggtggcatc
     3541 aacatccccg aggacatgga gcccggcctg accgccgaca ccttctacga cccgcccaac
     3601 ttcgtgttcc cgttcggggc gcacatctgc gtggtggagg tcgacaccga gaccggcaag
     3661 accggcgtgc gcgactactt cggcgtggac gactgcgggc cggtcatcaa cccgatcatc
     3721 gttgacggtc aggtgcacgg cggcatcgcg cagggcatcg cccaggcgct ctacgaggag
     3781 gccatctacg acgaggacgg tacgctcgtc accggctcca tggtcgacta catgatccct
     3841 ggggcccccg agctgcccaa ctacaccctg gaccggacgg tgacgccctc acccaccaac
     3901 gtgatggggg tgaagggcat aggcgaggcc gggaccatcg gctctccgcc cgccgtcatc
     3961 aacgcggtca tcgacgccct ctcgccgttc ggggtgacgc acatagacat gccggcctcc
     4021 ccgatgaggg tgtgggacgc catccagaac gccagggacg ggggtgaggc gtaatgtatc
     4081 cgatgccctt cgactacgag gtcgccgaat ctgtagatca ggccatagag ctcctcggtg
     4141 agcacggcga cgagtccaag ctgatcgcgg gcgggcactc cctgatcccg ctgatgaggc
     4201 tgcgtctcgc agccccgacg gtcctgatcg acctggaccg actggacgac ctgcgctacg
     4261 tgagcgacga gggcgaccat ctcgcgatcg gcgccctgac tcgccaccgc gaggtgctca
     4321 acaacccgct ggtgcaggaa cactgcggca tcctctcttt cacggccggc ctgcttggcg
     4381 acccctcggt tcagcaccgc gggaccatcg gcggcgccct ggcccacggc gacccctccg
     4441 gcgaccttcc caccgtcgta aagacgctcg agggcgagct ggtcatcaag ggacccgacg
     4501 gggagcggac catccaggcc gccgacttct tccaggacta cctgatgacc gaccttgacg
     4561 agcgggaggt catcacggag gtgagggttc caaagctgag ccccaatacc ggttgggcct
     4621 acaagaaatt cagccgccgg tcgcaggact gggccatcgt gggcgtcgct gccgtggtgg
     4681 agaagaacaa cggcaccatc ggctcggccc gcatcgggct gaccagcatg ggctcgaccc
     4741 ccatcagggc gagcgccgtt gaggaggccc tctccggcgc ctcccccgac ggggtggcag
     4801 aggcctcgga ggccgccgac gaggggacag agccggcctc tgacggcgcc gcctcggcgg
     4861 acttccggcg ccacctggcc agggtgtgga ccaggagggc cgtggaggag gccctgtctc
     4921 gttgaacgac gcagcgaacg gacgccgggg ggccctgccc cccggcgttt ctcgttcttc
     4981 ggagctctcc gcgaggctca gggagggcgg ctacctggct gacgagggcc tctcaaccgc
     5041 cgtcttcctc gccctcaaac ttggccgccc gctctttctg gagggcgagg ccggggtggg
     5101 caagacggag gttgcgaagg tgctctcgcg cgttctcgac acccccctca tccggctgca
     5161 gtgctacgag ggcatcgacg tgaaccaggc ggtctacgag tgggactacg ccaggcaact
     5221 cctgcacatc aggaccgcag aggccctcgg gatggccgag gaccgggcca gcttcgagga
     5281 cgagctctac acgaggcagt tcctggtcag caggccgctc ttgcgggcgc tggagcacca
     5341 tgggcccatg ccgccggtgc ttctgatcga cgagatagac agggccgacg acgagttcga
     5401 ggccttcttg ctggagatcc tctccgagtt cgccgtcacc atccctgagc ttggaaccat
     5461 aagggcagac cggccaccgg ttgccgtcct gacctccaac cggacccgag aggtccacga
     5521 cgccctcaag cggcgctgcc tctacttcta catcgaccac cctggcctgg agcgggaggt
     5581 ggagatagtg cgcctcaggg tgccgggggc cgaggagcaa ctggcccgcc aggtggccca
     5641 ggccgtccag aaccttcgtg tcatggacct gtacaagccc ccgggtatcg ccgagacgct
     5701 cgactggacc gaggcgctcg tggccctcgg ggccagggag ctcgacgagg atctgatctc
     5761 cgccaccctt ggatcggtcc tcaagtatcg cgaggaccag cagcgcacga tgggcatggc
     5821 cctcagcctg ttggctggcg gcggcaatgc ctgatacggc gacacccgag accggcccgc
     5881 ccctgatgct ggacgcggcc gtcaccttcg gccgcgtgct tcgccggatg gggctaccag
     5941 ccggtcctga tagggtggtc gagttcgtgc gggctgtgga agagctcgac gttaccaggc
     6001 gcgaggacgt gtactgggcg gggaggatca ccatgtgctc caacccggat cacctggagc
     6061 tatacgaccg cgcgttccgc ctgttctggg acggggagga accaccccgc atcgttccgc
     6121 ccccgaagtc cagcttctcc gcctccgcgg ccgaggactc cgtgcagccg ccgaagaaga
     6181 cggtcgagaa gagcgagcgg ggtgaggagg ccgtcgccct gcgctacagc cccgtggacg
     6241 ttctgcgcaa gaggaacttc gcggactgca cccccgagga gttcgccgag ctctaccggc
     6301 tcatggcgga cctgcgcctc gcgggggata cgcgcaaggg ccggcgcctc gggccggccc
     6361 ccaggggccg ccgcgacgcc aggaggaccc ttagggacgc ccttcgcacc ggcggcgagc
     6421 ccctgcgcca ccgctaccgc aaggcgaggg accagcccag gcggatcgtc ctgctgtgcg
     6481 acgtctccgg gtcgatggcc tcctacagcc gcaccctcct gcggttcatg cacgcgagcg
     6541 tcgtctcggg cgagcccgta gaggccttcg tgctcgggac caggcttacc cgcgtaaccc
     6601 gggaactggc cgaccgggac ccggaccggg ctatcggcga ggcctcgcgg gccgtcgagg
     6661 actggtccgg cggtacccgc ctcggcgaca ccatcaagga gttcgtggac cgctggggac
     6721 agcgcggtat ggcccgcggc gccgtggtgg tcatcctatc cgacggctgg gaccgcggcg
     6781 acgtgtcggt gctggcggag agcatggagc gaatcgcccg tctcgcccac agggtcgtct
     6841 gggtcaaccc cctcaaggcc gcaccaggct accggccgct tgccaggggc atggccgcgg
     6901 ccctcccgca cgtggacgtg ttcctctcgg gccacaactt cgagtccctg gaagagctcg
     6961 cccgggccat ctccggcgta gcagagggcg agagatagaa atggatataa gggcaacagg
     7021 catcagcatc ggactgcggt ccccggtgtg cttacaagac ccttgcttgc tgcacacggt
     7081 caggccgacg cgcaaacgcc acgtcgttcg agaccttacc tgaaacctac aaaggaggtg
     7141 cctctgtgaa tccagtggta gacaaggttg cgagctggcg cgaggagggc aagcaggccg
     7201 ccctcgccac cgtggtgaag gtcgagcgtt cggggccgct tgggccaggg acctctatgg
     7261 ccgtgtccga ggacggtcag gtcgccggtt ccgtcagcgg ggggtgcgtg gagcccgccg
     7321 tgttcgagga ggctatggcc gccatccaga gcggcgagcc gaagtacctc acctatggca
     7381 tctccgacga cgaggccgtt ggtgttggcc tgacgtgcgg tgggatcatc cacatcttcg
     7441 tcgaacgggt tgactggtag cggcatgggc gagcttctga ggcgctggca cgaggcgctg
     7501 gcggaccagt cgccggtggc gatggccacg gtcatccagg ggcctggaac ggggaacaag
     7561 cttctggtcg agcccgtggg ccacgtgggg acagcgggca acgacgaatt ggacaaggcc
     7621 gtggtggagg cggcgcgggg gctgctggag ggcggcagga cggagacgcg ccatttcggg
     7681 ccgaggggac agcggcgtat ggaggacgtg gccgtcttca tccagtcgtt cgccccgccg
     7741 cccaggatgt acgtgttcgg ggccatagat tttgcctctg ccctcgcccg cctcggcaag
     7801 ttcatgggct acagggtgac ggtctgcgac gccagggccg tgttcgcgac gcgcgagcgg
     7861 ttccccagcg tcgatgaggt ggtggtcgcc tggcccgacg agtttctcga gacggcgcag
     7921 gtggacaaac ggaccgtgat ctgcgtcctt acccacgacc ccaagttcga cgtccccgtg
     7981 ctgaaagcgg cgctggccac ggaggccgga tacatcgggg cgatgggcag tcgcaggacg
     8041 cacgacaacc gcacggcgcg cctgaaagac gagggcgcga ccgaggagga cctccggcgc
     8101 gtctccagcc ccatcgggct cgacatcggg gcgcgcaccc ccgaggagac ggcggtggcc
     8161 atcgccgcgg agatcatcgc cctgagaacc ggacactcgg ggggacggct cgccgagcgt
     8221 tcgggggcga tacatgcccc tgaagaagag ggggcgacgg cccgcaac
//
LOCUS       DBNGVD010000423         3826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_100944, whole genome shotgun sequence.
ACCESSION   DBNGVD010000423 DBNGVD010000000
VERSION     DBNGVD010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_100944"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="AC16CM_11400"
     CDS             complement(<1..788)
                     /locus_tag="AC16CM_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3140482.1"
                     /translation="MKYLVGLTADRGGQDALSLGRMLARTGDVTLTVCMVLPEVWGHP
                     SLARVDAEYAEFLKQHAEKTISEARETLGELVQAEFVTRPARSATDGLISAATEMDAS
                     LIVLGSARHGPIGRFTVGSVTNELLHSAPLPVVLTPRGHRPSSGARLRRVTCAFAGST
                     RSRTAFDAAVQLARRHDVPLRLTTLVVRDHQMYPSQVGYDAERLVEEQWHAQAAEAQE
                     EALAQVPGDVEVEAVVLSGRDWEHALDSLGWEEGEVLIVGSSRLG"
     gene            complement(785..2218)
                     /locus_tag="AC16CM_11405"
     CDS             complement(785..2218)
                     /locus_tag="AC16CM_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3140483.1"
                     /translation="MSTGNAGWRRLFRRKPVEAFVTETGPDAEGGELERSIGLFQLTM
                     FGVGATIGTGIFIVLTEAVPEAGPAVIFSFVLAGITAALTAVCYAELASTIPVSGSSY
                     SYTYATLGEITAFGVAACLLLEYGVSSAAVAVGWAQYLNELFASAFGFRLPETIIAAP
                     GEGGIFNLPAIVLVTLCCLLLIRGTRESATANTIMVLIKIAVLIFFAIIAFTAFDGSN
                     FTPFAPLGIAGIGVAASSIFFTFIGLDAVSTAGEEVHNPRRTLPLAIILATIIVTTVY
                     IIVAVAAVGAQRYQAFEGQAAGLAVILRNITGSDWAPAVLSAGAIISIFSVTLVTLYG
                     QTRILFAMSRDGMLPEIFHRLNQTTLVPVRNTLIVAAFVGILAGFVPLTVLIDLTSMG
                     TLVAFTVVSIGVIILRRTQPDLPRGFRVPFYPVTPVLSAAFALYIISQLPYSTFGLFA
                     VWLGVAAVIYFTYSRGHSRLADTEQAQ"
     gene            2557..>3826
                     /locus_tag="AC16CM_11410"
     CDS             2557..>3826
                     /locus_tag="AC16CM_11410"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3140484.1"
                     /translation="METARDWTGEDLRRLDEIVIEGERRFKERQPRSRAMIGEATGVL
                     AGGATSSWQIARPQMVWLSHGKGSRIFDVDGGEYVDLHGGYGVGAVGHGHPKIVEAIA
                     GRAPLGTHFAQPTEDAIVVAGELARRFGLPLWRFSNSGTEATMDAVHLMRAITGRDLI
                     VKVEGCYHGHHDSLMVSVSNEEEIGPSGRPFSVPSGTGIPRAITDLTLVVPYNDLGAL
                     ERVFADHPGEIAGMILEPIMMNAGVIAPEQGYLEGVREVVHAHGALLAFDEVKCGVTT
                     SYGGAAGYLGVRPDMVCLAKAMGGGVPCGAIGGTDEVMGRIADGTYEQVGTFNGNPLT
                     MAAARATLTEILTPEAYEHFDRLAGIMVEGSQAVIEEYELAAYPLSFGAKGSITFSES
                     RVRNYRDFLRLDDRFSQAHWLFQHNGGVFLP"
BASE COUNT          735 a         1214 c         1254 g          623 t
ORIGIN      
        1 ccgaggcggc tagatcccac gatcagcacc tcgccctcct cccaccccag gctgtccagc
       61 gcgtgctccc aatcgcgacc actcaggact acggcctcca cctcgacgtc gccaggcacc
      121 tgggcgagcg cctcctcctg cgcctcagcg gcctgcgcgt gccactgctc ttcgacgagg
      181 cgttcggcgt cgtagccgac ctgcgacggg tacatctggt ggtcgcggac gaccagggtg
      241 gtcagcctca agggcacgtc gtggcgcctg gcgagctgca ccgcggcgtc gaatgcggtt
      301 ctggagcgcg tggacccagc gaaggcgcag gtgacacggc gtagcctggc gccggaagac
      361 ggacggtggc cccgcggggt gagcaccacg ggtagcggcg ccgagtgcag aagttcgttg
      421 gtgacgctgc cgacggtgaa ccggccgatc gggccgtgcc tggcggagcc cagaacgatg
      481 agcgacgcgt ccatctcggt ggcggcggag attagtccat cggtagccga gcgcgctggc
      541 ctggttacga actctgcctg cacaagctcc ccgagggtct cgcgggcctc ggagatcgtc
      601 ttttcggcgt gttgcttcaa gaactcggcg tattcggcgt ccacgcgggc cagggaagga
      661 tgcccccaca cctcgggcag taccatgcac acggtcaggg tgacgtctcc cgtacgtgcc
      721 agcatccggc ccagagagag ggcgtcctgc ccgccccgat cagcggtgag gcctacgagg
      781 tatttcattg tgcctgctcc gtgtcggcca gccgcgagtg tcccctgctg tacgtgaagt
      841 agatgaccgc cgccaccccg agccagaccg caaacagccc gaacgtcgag tacggcagct
      901 gggagataat gtaaagggcg aaagctgcgc ttagcaccgg cgtgaccggg taaaagggca
      961 ccctgaaccc acggggaagg tcaggctggg tgcgccgcaa tatgatcaca cctatcgaca
     1021 ccacagtgaa ggcgaccagt gtccccatgc tggtcaggtc tatcagcacg gttagaggta
     1081 cgaacccggc gagtatcccc acgaacgcag cgacgatcaa ggtgtttcgt accgggacta
     1141 gggtagtctg gttgagcctg tggaagattt ctgggagcat gccgtcgcgg ctcatggcga
     1201 aaaggatgcg tgtctggccg tagagtgtca cgagcgtcac ggaaaagatg gatatgatag
     1261 cccccgccga gagcacggcc ggcgcccagt ccgaccccgt tatgttgcgc agaatcaccg
     1321 cgaggcccgc cgcctggccc tcgaaggcct ggtagcgctg cgcgccgacc gcagcaaccg
     1381 ccacgatgat gtagacggtg gtcactataa tggtcgcgag aatgattgcc agggggagcg
     1441 tcctgcgggg attgtgcacc tcctcgccgg ccgtcgagac ggcgtccagg ccaatgaagg
     1501 tgaagaagat cgacgacgcg gccacgccga tgccggcgat acccaggggc gcgaaaggcg
     1561 tgaaattgct accgtcgaag gccgtaaacg cgatgatggc gaagaagatc aagaccgcaa
     1621 tcttgatgag caccatgatg gtgttcgccg tcgcggactc ccgcgtgccc cggatcagca
     1681 agaggcagca cagcgtcacg agcacgatgg cgggaagatt gaatatacct ccctctcccg
     1741 gcgccgcgat aatggtctca ggcaaccgga aaccgaacgc gctagcgaac agctcgttga
     1801 gatactgcgc ccacccgacg gccacggccg ccgatgacac cccatactcc agcagcaaac
     1861 acgccgccac accgaaagcc gtgatctcgc ccagcgtggc gtaggtatac gagtaagacg
     1921 acccggagac ggggatggtc gaggcgagct ccgcatagca tacggccgtc agggcggccg
     1981 tgatgccggc cagcacgaac gaaaagatga ccgccggccc cgcctctggc accgcctcgg
     2041 tcaagacgat gaagatgccg gtgcctatgg tggccccgac accgaacatc gtcaactgga
     2101 atagacctat ggagcgctcg agctccccac cctccgcgtc agggcccgtc tccgtgacga
     2161 aggcctcgac cggcttcctc ctgaacagtc tccgccagcc ggcgttgccc gtgctcacgg
     2221 accccaccta acaatgagaa ccggccgacc ttgtgacctc ttcatggtgg acctccttgt
     2281 ggtggacctc cctgaccaat gactcatacc gtactatttg tgtacaggat aatgtctctt
     2341 gtgactgggt caagacacaa ttcacacata agctcgccga ggtgtcccgg tgtcggcatt
     2401 ccgcacacgc cagacagagt attaacccca cgcagggcac cccgatcggt gaggccaaga
     2461 tgccaaccag agagggccta aagccgcatc gtgctaccac gttgacacaa aggtgtctcg
     2521 tccttattat taaatagccg ttcaaaggag gaacgcatgg agaccgcgag ggattggacc
     2581 ggggaggatc ttcggaggct cgacgagatc gtcatcgagg gtgaacgccg gttcaaggag
     2641 cgtcagccgc ggtctcgggc aatgatcggg gaggccacgg gggttctcgc gggcggtgcc
     2701 acctctagct ggcagatcgc gcgtccgcag atggtctggc tcagccacgg taaggggtcg
     2761 aggatcttcg acgtcgacgg cggggagtac gtggacctcc acggtggtta cggcgttggc
     2821 gccgttggcc acgggcaccc gaagatagtc gaggccatcg ccggcagggc gccgctcggg
     2881 acgcacttcg cccagccgac ggaggatgcc atcgtggtcg ccggcgagct ggccaggcgg
     2941 ttcgggctac cattgtggcg gttctcgaac tccgggaccg aggcgaccat ggacgccgtt
     3001 cacctcatgc gcgccattac ggggcgggat ctcatcgtca aggtcgaggg gtgctaccac
     3061 gggcaccacg actcgctcat ggtctccgtc tccaacgagg aggagatcgg gccgtctggg
     3121 cggcccttca gcgtgccgtc ggggacgggc ataccgcggg ccatcacgga cctcaccctg
     3181 gtcgtgccct acaacgacct gggcgcgctt gagagggtct tcgccgatca tccgggcgag
     3241 atagcgggca tgatcctgga gcccataatg atgaacgccg gggtgatcgc ccctgagcag
     3301 ggttatctgg agggcgtgcg cgaggtggta cacgcgcacg gcgccctgct agccttcgac
     3361 gaggtcaagt gcggcgtcac cacgtcctac gggggggctg ccggttacct gggcgtacgg
     3421 cccgacatgg tctgcctcgc caaggcgatg ggcgggggag tgccctgcgg ggccatcggt
     3481 ggaaccgatg aggtcatggg ccgcatcgcc gacggcacct acgagcaggt cggcaccttc
     3541 aacggcaacc cgctcaccat ggccgccgcc agggcgacgc ttaccgagat cctcaccccc
     3601 gaagcctacg agcatttcga ccggctggcc gggatcatgg tggagggttc gcaggccgtt
     3661 atcgaagagt acgagctggc cgcctacccg ctatctttcg gggccaaggg ttcgatcact
     3721 ttctccgaaa gcagggtgcg taactaccgg gatttcctcc ggctggacga ccggttcagc
     3781 caggcacact ggctcttcca gcacaacggc ggcgtgttct tgccgc
//
LOCUS       DBNGVD010000424         4297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1923650, whole genome shotgun sequence.
ACCESSION   DBNGVD010000424 DBNGVD010000000
VERSION     DBNGVD010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1923650"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="AC16CM_11415"
     CDS             complement(<1..1417)
                     /locus_tag="AC16CM_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3140485.1"
                     /translation="MSTDTRSPAAHVADGHDLIRVHGARENNLKDVGIEIPKRRLTAF
                     TGVSGSGKSSLVFGTIAAESQRLINETYSAFLQGFMPTLTRPEVDLLEGLTTAIIVDQ
                     ERMGGDVRSTVGTATDTGAMLRILFSRLGKPYVGPPSAFAFNVPSVKASGAITVERGG
                     KSRAEKATFERLGGMCPRCEGIGSVSDFDLSALYDESKSLNEGALTIPGYSMDGWYGR
                     IFSGAGFDMDKPIEKFTRKESHKLLYGEPTKIKVEGINLTYEGVIPKIQKSMLSKEID
                     ALQPHVRRFVERAITFDTCPDCGGTRLAAEARSSKIEGINIAEACAMQISDLARWVRG
                     LDEPSVAPLLAALGESLDSFVEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGSSLTDV
                     TYVFDEPTIGLHPHDIQRMNDLLLRLRDKGNTVLVVEHKPEVISIADHVVDLGPGAGT
                     DGGRVMFEGNVEGLRRSGTLTG"
     gene            complement(1452..1862)
                     /locus_tag="AC16CM_11420"
     CDS             complement(1452..1862)
                     /locus_tag="AC16CM_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140486.1"
                     /translation="MDIVIHASFLPHVDPEASLAFYRDALGFEVRNDVGYGGMRWLTV
                     GPAGQPGTSIVLHPPGADPGITEEERRTIAEMMAKGTYSGILLATADLDGTFARLQES
                     VAEIVQEPTDQPYGVRDCAFRDPAGNMVRIQELG"
     gene            complement(1904..2347)
                     /locus_tag="AC16CM_11425"
     CDS             complement(1904..2347)
                     /locus_tag="AC16CM_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140487.1"
                     /translation="MTGGPNVAQHLGDLARLRRVRDRIDREYDRPLDIEALARGAHMS
                     AGHLSREFRRAYGESPYSYLMTRRIERAMALLRRGDLSVTEVCFSVGCSSLGTFSTRF
                     TELVGVPPSVYRREAARATAGMPSCVAKQVTRPIRNREVRPRGRT"
     gene            2554..3096
                     /locus_tag="AC16CM_11430"
     CDS             2554..3096
                     /locus_tag="AC16CM_11430"
                     /inference="COORDINATES: protein motif:HMM:NF047262.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HIR3140488.1"
                     /translation="MDDDRRERFVAFGRRLMARGRVTVLALFGLTDRGGWVAPGVLGC
                     AYPRTERSLAALSADGVRLLVNLHERPQAPVLLARSGLGEIHLPVKDFAAPSLAQIER
                     GVNAVFEARATGDAVAVHCGSGLGRTGTLLACYLASSEGLSAEEAIQRVRAIRPGSVE
                     TRSQAATVYAWTRRQRPTPH"
     gene            complement(3209..3502)
                     /locus_tag="AC16CM_11435"
     CDS             complement(3209..3502)
                     /locus_tag="AC16CM_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140489.1"
                     /translation="MHVLKIEYPVADYDTWKASFDRDLLDREGSGVRRYRVLRATDDP
                     NYVLIDLDFDKESEATAYLAALQRQFYSSQQASPVSGGGLQTRIVEVAEAKEY"
     gene            complement(3526..>4297)
                     /locus_tag="AC16CM_11440"
     CDS             complement(3526..>4297)
                     /locus_tag="AC16CM_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140490.1"
                     /translation="SAAFSSGPVDGLGPIRNPLGIEGFSSVYNSILITMAATVLVVAA
                     ALSVFMRLRRARGIERQQIKWFAYAAAATVMGLVLTRVIPDVIDVPLRIERIGNALFL
                     LLIPAMPISIGIAILRFRLYEIDTLINRTLVYGSLTATLVATYFGGVLTIQTLFQAVT
                     GQEQQPQLAVVVSTLAIAALFNPLRRRTQSFVDRRFYRSKYDAGKTLEAFSTRLRDET
                     NLQALKKDLVEVVRETMQPAHISLWLHPDKAQESKQTR"
BASE COUNT          705 a         1382 c         1406 g          804 t
ORIGIN      
        1 gccccgtcag cgtgccgctt ctgcgcagtc cttcgacgtt gccctcgaac atcacgcggc
       61 ccccgtctgt gccggcgccg gggccgaggt cgaccacgtg gtcggcgatg gagatcacct
      121 ccggcttgtg ctcgacgacc agcacggtgt tgcccttgtc gcgcagccgc agcagcaggt
      181 cgttcatccg ctggatgtcg tgcgggtgca gccctatggt tggctcgtcg aagacgtagg
      241 tgacgtccgt gagcgacgaa ccgagatgcc ggatcatctt ggttcgctgg gcctcaccgc
      301 ccgagagcgt gcccgagggg cggtcgagcg agaggtagcc aagccctatc tccacgaacg
      361 agtcgaggct ctccccaagc gccgcaagca gcggcgccac cgatggctcg tccagcccgc
      421 gcacccaccg ggcgaggtcg ctgatctgca tcgcgcaggc ctcggctatg ttgataccct
      481 cgatcttcga agaacgggcc tccgcggcga gcctggtgcc gccgcagtca gggcaggtgt
      541 cgaaggtgat tgcccgctcg acgaagcggc gaacgtgcgg ttgcagcgcg tctatttcct
      601 tggagagcat cgacttctgg atcttgggaa tgacaccctc gtaggtcagg ttgatgccct
      661 cgaccttgat cttggtcggc tccccgtaga gcagcttgtg ggactctttc ctggtgaact
      721 tctctatagg cttgtccatg tcgaagccgg caccgctaaa gatgcggccg taccagccgt
      781 ccatgctgta gccgggaata gtgagcgcgc cctcgttgag cgacttgctc tcgtcgtaca
      841 acgccgacag gtcgaaatcg gagaccgatc ctatcccctc gcagcgcgga cacatgccgc
      901 cgagtcgctc gaacgtcgcc ttctcggccc tgctcttgcc gccccgctcg accgtgatag
      961 caccgcttgc cttgaccgat gggacgttga aggcgaacgc gctcggcggg cccacgtaag
     1021 gcttcccgag cctgctgaac aggatgcgca gcatcgcgcc cgtgtcggtg gcggtgccga
     1081 ccgtggagcg gacatcgccg cccatccgtt cctggtcgac gatgatcgcg gtcgtcaacc
     1141 cttcgagtag atctacctcg ggccgcgtga gggttggcat gaagccctgc aagaaggcgc
     1201 tgtaggtctc gttgatcagc cgctgcgact ccgcggcgat ggtgccgaac accagggagc
     1261 tcttgcccga gccggagacg ccggtgaacg ccgtcagccg gcgcttcggg atctcgatgc
     1321 cgacgtcttt gaggttgttc tcgcgcgcgc cgtgcacgcg gatcagatcg tggccgtcgg
     1381 cgacatgcgc cgcgggtgag cgggtatccg tgctcatcgt gcctcctgtc tgccgggccg
     1441 tcccatgcgg ctcagcccag ctcctggatg cggaccatgt tgccagcggg gtcgcggaag
     1501 gcgcagtcgc gaacgccata gggctgatcg gtcggttcct ggacgatctc ggcgacgctt
     1561 tcctgcagcc gcgcgaaggt gccgtcgagg tcggcggtag ccaggaggat gccggagtag
     1621 gtgcccttgg ccatcatctc ggcgatggtg cgacgctcct cttcggttat acccgggtcc
     1681 gcgcccggcg gatggagcac gatggacgtt ccgggctgcc cggcagggcc gaccgtgagc
     1741 cagcgcatcc cgccgtaccc gacgtcgttc ctgacctcga agcccagggc gtcacggtag
     1801 aaggccaggg aggcttccgg gtctacgtga gggaggaagc tcgcgtgaat gactatgtcc
     1861 atgccggtcc tttcatcgaa gggcgaggtc cattacggtc attctacgta cggccccgtg
     1921 gcctcacttc tcgattcctg atcggtctgg tcacctgctt ggccacgcag gacggcatac
     1981 ccgccgtcgc gcgcgccgcc tcacgccgat agacgctggg cggcaccccg accaactcgg
     2041 tgaaccgggt gctgaacgtg ccaagtgacg aacagcccac actgaagcag acctcggtta
     2101 cgctcaggtc cccgcgacgc agcagcgcca tggcacgctc gatgcgtcgc gtcatcaggt
     2161 aggagtaggg agattctccg taggcgcgcc ggaactcgcg gctgaggtgc cccgccgaca
     2221 tgtgggcccc gcgcgcgagc gcctcgatgt ccagcggacg atcgtactcc cggtcgatac
     2281 gatcccgcac gcgacgcagc cgcgcgaggt cgccaaggtg ctgcgccacg tttggtccgc
     2341 cggtcatccg tacgatcatg ccacgccgta gtagagtgct caagaggcgg tttccaggct
     2401 tctggatatt ggacagccct gacacgattt gcccggtatc cgtagtctcc tgctccaccc
     2461 cgggatgctc gaagattttc ccgcccccgg tctcaccggc ccgaagcgtg ggaaccggtg
     2521 gtcctcgcgg tggccgggcg ttaggcttca tgggtggacg atgatagaag ggagcggttc
     2581 gtggcgttcg ggcgtcgctt gatggcacgg gggcgggtca ccgtcctcgc gctcttcggc
     2641 ctgaccgaca ggggcgggtg ggttgcgcct ggcgtgctcg ggtgcgccta cccgcgtacg
     2701 gaacgatccc tcgccgcgct ctccgcggac ggcgtgcggc tcctggtcaa cctccacgag
     2761 cggccccaag cccccgtcct ccttgctcgt tctggcctcg gggagatcca cctcccggtg
     2821 aaggactttg ccgccccctc gctcgcccag atcgaacgcg gcgtcaacgc cgttttcgag
     2881 gcccgggcga cgggcgacgc ggtggcggtc cactgtggga gcggcctcgg acgcaccggc
     2941 accctgctcg cctgctacct ggcgagcagc gaagggctca gcgcggagga ggcgattcag
     3001 cgcgtccgtg ctatcaggcc cggatccgtg gagacccgct cccaggccgc aacggtctac
     3061 gcgtggaccc gccggcaacg accaaccccc cactgagtgg gggtggccgc caccctggtg
     3121 ggcgttttgt gcgagatcct gcacccgggc ttcggagaat tcaccgtcca ggcactaggg
     3181 tgaatacgcg agcgtccgta ggttgggatc agtactcctt ggcctccgcc acctcgacga
     3241 tacgtgtctg caggcctccg cctgagaccg gggatgcctg ctgtgacgaa tagaactggc
     3301 gctgcagcgc ggcgagataa gctgtcgcct cgctttcctt gtcgaagtcg aggtctatca
     3361 agacgtagtt gggatcgtct gttgcgcgaa ggactcggta gcggcgcacg ccagaacctt
     3421 ctcggtcgag caaatcacgg tcgaaggacg ccttccacgt gtcgtagtcg gccaccgggt
     3481 actcgatctt caagacgtgc atgcctcgcg ctcccttctc tagaattaac gtgtctgctt
     3541 gctctcttga gccttgtcgg gatgcaacca cagcgagatg tgggcaggtt gcatcgtctc
     3601 ccttaccact tccaccagat ctttcttcaa tgcctggagg ttcgtctcgt cccttagcct
     3661 ggtggagaag gcttccagtg tcttcccggc gtcgtacttg ctgcggtaga agcggcggtc
     3721 cacgaaggac tgggtgcgcc gcctcagcgg gttgaacagg gcggcgatgg cgagcgtcga
     3781 gacgaccacg gcgagttggg gctgctgttc ctgcccggtt acggcctgaa agagcgtctg
     3841 gatcgtgagc accccaccga agtaggtcgc caccagcgtg gctgtgagcg agccgtagac
     3901 cagggtgcgg tttatgaggg tgtctatctc gtagagcctg aagcgaagga tggctatccc
     3961 tatggagata ggcatggccg gtatcaacaa gaggaaaagc gcgttcccta tccgctcgat
     4021 cctcaggggc acgtctatca cgtcggggat gacgcgggta agtacgaggc ccatgaccgt
     4081 cgccgcagcg gcgtaagcga accacttgat ctgctggcgc tctatccctc tggcccgacg
     4141 caggcgcata aatactgata aagctgccgc aacgacgaga acagtagccg ccatggtgat
     4201 gagaatcgag ttgtagacgc tggagaagcc ctctatgccg agcgggtttc gtatcgggcc
     4261 aagaccatct accggaccag aagagaacgc agccgat
//
LOCUS       DBNGVD010000425         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3745378, whole genome shotgun sequence.
ACCESSION   DBNGVD010000425 DBNGVD010000000
VERSION     DBNGVD010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3745378"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..387
                     /locus_tag="AC16CM_11445"
     CDS             202..387
                     /locus_tag="AC16CM_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140491.1"
                     /translation="MNIRQANGIGRVHVAMPGMRQTYCGRTIDDEDWVTTSKEANCTG
                     CARAGAPAPEEPANKGR"
     gene            504..1514
                     /locus_tag="AC16CM_11450"
     CDS             504..1514
                     /locus_tag="AC16CM_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3140492.1"
                     /translation="MKIPGRQLIEKAQSKGNEAEVPMAENLGLVDFAKLTYKEMNEDH
                     VMAFAGNLTYKALFAVFPFFTFLLSLLGIFSATDLVNTAVENLSGAVPDSAKTFIEDQ
                     LLGITQSQAESAFTFGAIVSVALALWGVSGAFRSVMEAMNVMYSVEEDRPFWKVYGLS
                     IFMSLAVVILLVGALVLVVFGESIGVAVADVIGLGSVFATLWSILQWPVVACVVLFAF
                     SLIYYFAPAARQRFRWVSPGSLIAFVFWLLFSLLFSLYASTIGGDSYNETYGSLAGII
                     VLMLYVYYSALIMLIGAEMNQVIEWHIPGGKNEGEKVPEADRKPDVHRLNREEGQEGE
                     GR"
     gene            1532..>2259
                     /locus_tag="AC16CM_11455"
     CDS             1532..>2259
                     /locus_tag="AC16CM_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016163.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR3140493.1"
                     /translation="MKIYGLDFTSSPGRKKPLIVLDCRLEDESLRIEGSETLTDFDGF
                     EEFLRTPGPWVCGLDFPFGQPRSLVEALGWPAGWEGYVGEVGGLSKDEFEDMIRADMA
                     TRPPGSKWRYRLADRRSRSSSAMMLFRVPVGKMFYQGAPRLLASGVNVEPCRRNGDDR
                     VAVEAYPAVVARRFLGRTAYKRDAVPDTPERKSARETLLAGLESRALEQTYGFTVEMD
                     DRWKQEFVSDPSADALDSLLCAVQA"
BASE COUNT          406 a          710 c          743 g          400 t
ORIGIN      
        1 agaccgccaa ccgcgaggca gcaagacccc tccttccggc cgaacgcggc gatctcacca
       61 ggctctgtgc gcccctcggg tgcagacccc agcacacgat ggacggtgcc gatcccgtcc
      121 gtctcgcgcg ttccgggcgg gggagcttag caggcgaaga gaacgagtag actcttgggg
      181 caagcgtcga gaaggagaaa catgaacatt cgtcaggcga acgggatcgg cagggtacac
      241 gtggccatgc cggggatgcg gcagacatac tgcggcagga cgatcgacga tgaagactgg
      301 gtaaccacct ccaaagaggc aaattgcacc ggttgcgcca gggctggagc gcccgcgccg
      361 gaggagccag ctaacaaggg gcgctgatcc gaggcccacc ccctctcgta ccggttgtta
      421 gatgggtagc ccctcgcggg tatagaagcg taaagagatg atgcgaggct ggcgtgaggt
      481 gtatgcctgc gggaggaaca cgcatgaaga taccgggtag acagttgatc gagaaggcgc
      541 agagcaaggg taacgaggcc gaagttccga tggcggagaa cctggggctc gtggacttcg
      601 ccaagctgac gtacaaggag atgaacgaag accacgttat ggccttcgcc ggcaacctca
      661 cctacaaggc gctgttcgcg gtcttcccgt tcttcacctt cctgctctcg ctgctcggta
      721 tcttcagcgc caccgacctg gtcaacacgg cggtcgagaa cctctccggg gcggtgcctg
      781 attcggccaa gacgttcatc gaggaccagc ttctgggcat cacgcagagc caggccgaga
      841 gcgccttcac gttcggggct atcgtctcgg tggcgctcgc cctctggggc gtctcagggg
      901 cgttccgctc cgtgatggag gcgatgaacg ttatgtactc agtggaggag gacaggccgt
      961 tctggaaagt gtacgggctc tccattttca tgtccctggc ggtcgtcatc ctgctcgtcg
     1021 gggcgctcgt gctggtggtc ttcggcgagt ccatcggcgt tgccgtcgcc gacgtcatcg
     1081 ggctcgggtc tgtcttcgcc acgctctgga gcatcctgca gtggcccgtc gtcgcctgcg
     1141 tcgtgctctt cgcattctcc ctcatctact acttcgcgcc ggccgccagg cagcggttcc
     1201 gttgggtcag ccccggctcc ctcatcgcct tcgtgttctg gctgctcttc tccttgctct
     1261 tctcacttta cgcgagcacc atcggcggcg actcctacaa cgagacctac gggtcgctcg
     1321 ccggcatcat agtcctgatg ctctacgtct actactcggc cctcataatg ctcatcggag
     1381 ctgagatgaa ccaggtgatc gagtggcaca tccccggcgg caagaacgag ggcgagaagg
     1441 tcccggaggc cgaccgcaaa ccggacgtcc accgcctgaa cagggaggag ggccaggaag
     1501 gcgagggccg gtagccccac atcgcacagg catgaagatc tacggcctcg actttacgag
     1561 ctccccgggc cgcaagaagc cgctcatagt gcttgactgc cggctagagg acgaatccct
     1621 gaggatagag ggctcggaga cgctgaccga cttcgacggc ttcgaggagt tcctgcgtac
     1681 gccgggaccc tgggtgtgcg gtctcgactt cccttttggg cagccgcggt ccctggtcga
     1741 ggccctcggc tggccagcgg gctgggaggg ctacgtgggg gaggtcggcg ggctctccaa
     1801 ggacgaattc gaagacatga tacgagccga catggccacc aggccacctg gcagcaagtg
     1861 gcgttacagg ctcgccgacc gcaggagccg ctccagcagc gcgatgatgc tcttccgggt
     1921 gccggtcggg aagatgttct accagggggc gccccgcctg ctcgcctcgg gcgtcaacgt
     1981 ggagccctgc cggcgtaacg gggatgaccg cgtcgccgtc gaagcctacc cggcagtcgt
     2041 ggcccgccgc tttctcggcc gtaccgccta caagcgggac gccgtgccag acacgccgga
     2101 gaggaagtcg gcccgcgaga cgttgcttgc cggcctggag tcaagggcac tcgagcagac
     2161 ctacggtttc accgtggaga tggacgaccg ctggaagcaa gagttcgtct cggacccatc
     2221 cgccgacgcc ctcgactcgc tgctttgcgc ggtgcaggc
//
LOCUS       DBNGVD010000426         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_284216, whole genome shotgun sequence.
ACCESSION   DBNGVD010000426 DBNGVD010000000
VERSION     DBNGVD010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_284216"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="AC16CM_11460"
     CDS             <1..1380
                     /locus_tag="AC16CM_11460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR3140494.1"
                     /translation="IGTARTFPDKVNEEAGAVSSRGLGERRDLRDLPTVTIDGADAKD
                     FDDAISVERTGDGYRVWVHIADVTHYLEPGSALDKQARFRGNSVYLPSTVAPMLPSRL
                     SDDVCYLRPHEERAAVTVEMEVTGEGETKSFRAFRSVILSDARLTYDGVDAFLRDDGA
                     VENPALVTIAYELSRRLKVRAARRGKLELGGKEPEYQVDETGVPLGAGVRRSTPAREM
                     IEELMVLANEAVAKELRHKKGGVFRVHERPDEESMEVLAGRLAAVGMQAEPTPENLGT
                     ISQMAKSDAVNYLILRSIPRAVYAPDDLGHYGLALEHYTHFTSPIRRYADVLVHRALL
                     DDEPPEDLAGVASHISEREYRSMICERTADQYTLMWLMRDRVGDVLEGTVVSTASFGL
                     FVELETGATGLVHVSKLPGWWELEPNGVVLSNDSIGASYRVGDRVLVELLDVLPLMQR
                     AELRVVKHL"
     gene            1377..1829
                     /gene="smpB"
                     /locus_tag="AC16CM_11465"
     CDS             1377..1829
                     /gene="smpB"
                     /locus_tag="AC16CM_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3140495.1"
                     /translation="MTDFARNKKALHDFSIDETYEAGIQLTGPEVKSIREGRANLKES
                     YARIRDGELFLIGAHISPYANARQERQIPTRDRKLLLHRKEIDRLIGKSQEEGKTLIP
                     LKLYSKHGIVKLQIAVASRKREYDKRREIAKKTADREIQRAMKERLRR"
     gene            1943..2296
                     /gene="ssrA"
                     /locus_tag="AC16CM_11470"
     tmRNA           1943..2296
                     /gene="ssrA"
                     /locus_tag="AC16CM_11470"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2703..>3520
                     /locus_tag="AC16CM_11475"
     CDS             2703..>3520
                     /locus_tag="AC16CM_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3140496.1"
                     /translation="MNERTGKALTRRDFLRAAGAGAAGMGLLGAAGCSGLADRMPRLP
                     EEYLPGGGGGPNVILVILDSLRRDHVGAYGNSWIHTPTLDAIATESLRFTHAQPEAMP
                     TLPVRRAIYTGMRTWPTSPPHFGWSAIPSGQHTLAEILDEQGYNTSLVTDAYVQFPHN
                     FSRGFGLYREIRGQENDPYRDPASIDEEWMRRNYIVLGEGNKARQYLANVQDRDGEKD
                     YFAPRVFSEATDALEKAARDHRPFFLVADCFDPHEPWDPPGEYVALYDQDYDGR"
BASE COUNT          704 a         1094 c         1143 g          579 t
ORIGIN      
        1 atcgggacag cccgtacgtt cccggacaag gtgaacgagg aggctggggc cgtctcaagc
       61 cggggtctgg gggagcgaag ggacctgcga gatcttccca cggttaccat agacggcgca
      121 gacgccaagg atttcgatga tgccatctcg gttgagcgga ccggcgacgg gtacagggtg
      181 tgggtgcaca tcgccgacgt gacgcattac ctggaacctg gctctgcgct ggacaaacag
      241 gccaggttca ggggaaactc cgtctacctt cctagtacgg tcgcgccgat gcttccctca
      301 cgactctccg atgacgtctg ctacttgcgc ccgcacgagg agcgggcggc ggtgacggtc
      361 gagatggagg tcaccggtga gggcgagaca aagagcttca gggcttttcg aagcgttatc
      421 ctgagcgatg ccaggctcac ctacgatggt gtggatgcct tcctgaggga tgatggcgcc
      481 gtggagaatc cggctctcgt cacgatcgcc tacgagctct cgcgcaggct caaggtgcgc
      541 gccgcgcgca gaggcaagct ggagctcgga gggaaagagc ccgagtacca ggtagacgaa
      601 accggggtcc ccctcggtgc cggggtacgc cggtccacgc cggcccggga gatgattgag
      661 gagctcatgg tcctcgcgaa cgaggcggtc gcgaaggagc tgaggcacaa gaagggcggc
      721 gtcttcaggg ttcatgagcg gccggacgag gagtcgatgg aggttctggc cggaaggctt
      781 gcggccgtcg gcatgcaggc ggagcccacg cccgagaacc tgggcaccat ctcccagatg
      841 gccaaatcgg atgcggtgaa ctacctgatc ctgcgttcca tcccgcgcgc cgtctacgct
      901 cccgacgacc ttggtcatta cggcctggcc ctggaacact acacgcactt tacctcgccc
      961 atcaggcgct acgccgacgt gctcgtgcac cgcgccctcc tggacgatga gccacccgag
     1021 gacctggccg gggtcgcgtc ccacatctcg gagcgggagt accggtccat gatctgcgag
     1081 cgcaccgccg accagtacac cctgatgtgg ctcatgcgcg accgggtggg ggatgtgctc
     1141 gagggcaccg tggtcagcac cgcctccttc ggcctcttcg tcgagctgga gacgggggct
     1201 acgggcctcg tacacgtgag caaactcccc ggctggtggg agctcgagcc aaacggcgtc
     1261 gttctctcca atgactcgat aggggcctcc tacagggtgg gggacagggt cctggtagaa
     1321 ttgctcgacg ttctcccgct catgcagcgg gccgagctga gggtggtgaa acacttgtga
     1381 cggacttcgc acgcaacaag aaggcgctgc acgacttcag catagacgag acctacgagg
     1441 ccggcatcca gttgacggga ccggaggtca agtccatcag ggagggcagg gccaacctca
     1501 aggagagcta cgcgcggatc agggacggcg aactgttcct gatcggggcc cacatctccc
     1561 cctacgccaa cgccaggcaa gagcgccaga tcccaacccg cgaccgcaag cttctgctgc
     1621 acagaaaaga gatagaccgc ctgataggaa agagccagga ggaaggcaag accctgatcc
     1681 ccctcaagct ctactccaaa cacggcatcg tcaagctcca gatagccgtg gccagccgca
     1741 aacgcgagta cgacaaacga cgcgagatcg ccaaaaagac ggccgaccgc gagatacaac
     1801 gcgccatgaa agagcggctt cgccgctagc ttttcagcta tcagccatca gcggtcagct
     1861 ctcctgtact tgctgaaagc cgaaagctga tcgctgacag cttgctgggt gcctgcaaaa
     1921 tgataaaata tacaccgtac tagggggcgc ataggcttcg acgggagtgg tcagcgtcct
     1981 gtgagacgag ccgagtttgc tggagactcg taaaacgccg gcaccacaac acacacgccg
     2041 acgataatct cgcggtggct gcctaactag agagcagccc atcggtccgg gcggcccacg
     2101 gcccggttcc cggtgtcatt tagtgggctc accctccggg ttctggcccg gtgcacggag
     2161 ggaacatcta acgggctagc cccgcggcgg cgccccggtc cgtcgcgaaa ggcgaaacaa
     2221 aaccgggagc tacgctcgta gaatctcacg gacggcgcac tcgcggacgc gggttcgaat
     2281 cccgccgcct ccactcgagc gggccggctt tgccggcccg cgcacgctcc ggcaaacgcc
     2341 tacgcttgtc agcacactac gccctccggg ctccgtttgt cagcacgctc cggctagcgc
     2401 cctcgcttgt cagcaacgct tcggcttcgc ctcgctttcg gccagtcgtc tcgattcgca
     2461 acggtggctt cgacaaccaa ccttttccgg gtatcgaatc gttagagcgg aatagtgcag
     2521 gccgcagagg gatctggctg acagcgagcc aggcggagcc gggcgaagcg ctgctgacaa
     2581 gcgaccgaag ggagcgtgct gacaagccgc gaagcggcgt gctgaaagcg gagcccgcaa
     2641 gggcgtagcg tgcttttctg ctacagtttc ctttttggga gcctcgcaga gatttttctg
     2701 gagtgaacga gagaacgggt aaggcgctaa cgcgcagaga tttcctgagg gccgccggtg
     2761 cgggagcggc tggcatgggc ctgctcggtg cggcggggtg ctccgggctt gcggatagga
     2821 tgccacggtt gcccgaggag tatctgcccg gcggtggggg cgggccgaac gtgatcctgg
     2881 tgatcctgga cagcctcagg cgggatcatg tcggcgccta cgggaactcc tggatacata
     2941 cgcccacgct cgacgccatc gccacagaga gcttgcggtt cacgcacgcc cagcccgagg
     3001 ccatgcctac cttgccggtc aggcgcgcca tctacacggg gatgcgaacc tggccaacca
     3061 gccctcccca ctttggatgg tccgccatac cctcggggca gcacacgctg gcggagatcc
     3121 tcgacgagca gggctacaac acctccctcg tgacggatgc ttacgtgcag ttcccccaca
     3181 acttcagtcg gggcttcggg ctgtaccgtg agatccgggg ccaggaaaac gacccctaca
     3241 gggatcccgc ttcgatcgac gaagaatgga tgcggcgaaa ctacattgtc ctgggggagg
     3301 gcaacaaggc ccgtcagtac ctggccaacg tacaggaccg tgatggcgag aaagactact
     3361 tcgccccgag ggttttctcg gaggccaccg acgcactgga gaaagccgcg agggatcatc
     3421 gacccttctt tctggtggca gactgcttcg acccgcacga gccctgggac ccgcctgggg
     3481 agtacgtggc gctctacgac caggactacg atggaaggga
//
LOCUS       DBNGVD010000427         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_284599, whole genome shotgun sequence.
ACCESSION   DBNGVD010000427 DBNGVD010000000
VERSION     DBNGVD010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_284599"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16CM_11480"
     CDS             complement(<1..851)
                     /locus_tag="AC16CM_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140497.1"
                     /translation="MGLSSFGLGAAGMFATVYCTQAILPTLEREFGVGAARSGLTISA
                     VVVAMAVGAWIWGPLSDRIGRKRCLVLSSGLLVAPTVGLGLAPNFTVLLLCRLLQGLC
                     MPGLLAVGLNYVQEVFGDWLGGKAMGCYLSSLVAGGLIGRVGVALATQEVGWRLSLAS
                     LALLPSVAAVFMYYALPQSSLPTRQKQKLAAVVRLLGNRALLLATLGGCALFFSFVGV
                     FSFVTFYVEAPPFSLSPATGSLIFLLWSFGAVGPLAGAVADRVGWRATLAGALAGSLL
                     ALPISLLG"
     gene            1462..1863
                     /locus_tag="AC16CM_11485"
     CDS             1462..1863
                     /locus_tag="AC16CM_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140498.1"
                     /translation="MTKTNHYLYALAALMTLVMLAALTATMTSNPVQAATKARTTLTI
                     QAEGVDLFGKVKSTRLGCLGNRTVKVYKQKGTEQSPSTDPVIASDTSERQGDVGVWSV
                     GNTGMEGKFYARTRATLNCKGDTSPTILARR"
     gene            1939..>2993
                     /locus_tag="AC16CM_11490"
     CDS             1939..>2993
                     /locus_tag="AC16CM_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3140499.1"
                     /translation="MFHTRANAMLGLKGLFLGQLIAVFIMLLALATPAQAQLTFPVTR
                     TDDPSPQGCFPGDCSLREAVIAANGTPGSDTIVLEPTTYNLTIAGNGGADQGDLDITE
                     NLDIESTGPGDATINANRVDRVLEVVSGGLSLRDITVRNGLATPDGQGFLRGGGIRVF
                     GSLYLYDSKVVDNHMPTVNGADGGGIYNDGHTVIMRSEVSNNTAVQGFGGGIYTPANG
                     VTEIRDSKLSGNEAVFGGALASASGGGNVMVERSQLSLNTAGLGGASYQLGSSTYTFT
                     NTTLDGNTATDGTGGGAIRVRSGSATLNNSTVTRNEAPNGGGLAAVNDGSSITIANSI
                     VAGNTDTNQADGSFPDCL"
BASE COUNT          641 a         1026 c          924 g          402 t
ORIGIN      
        1 ccgagcaggg agattggcag cgccagcagg cttcccgcca gggcgccggc gagcgtggcc
       61 cgccatccga cacggtcggc gacagccccg gcgagggggc ccaccgcccc gaagctccag
      121 agcaggaaga tgaggcttcc cgtcgccgga gaaagcgaga agggaggggc ctcgacgtag
      181 aaggtgacga aggaaaacac gccgacgaag gagaaaaaca gcgcacatcc ccccagcgtc
      241 gccaggagca acgcacggtt tccgagcaga cgtacgacgg ccgcgagctt ttgcttctgc
      301 ctggtcggga gggaggactg gggcaacgcg taatacatga acaccgccgc cacgctgggc
      361 agcagggcga ggctagccag ggagaggcgc catcccacct cctgggtcgc caacgccacc
      421 ccgacccggc cgatcaggcc tcccgctacc agggaggaca ggtagcagcc catggctttg
      481 cccccgagcc agtccccgaa gacctcctgc acataattaa ggcccacagc tagcaggcct
      541 ggcatgcaga gaccctgcag gagcctgcac agcagcaaga cggtgaagtt cggggcgagt
      601 ccaaggccta cggtcggggc caccagcaga ccgctggaga gaaccaggca tcgtttgcgt
      661 ccgattctgt ctgagagcgg gccccagatc caggcgccaa cggccatggc aaccacgacc
      721 gcggagatgg tcagtcccga ccgcgcggca cccaccccga actcccgttc cagggtcggc
      781 aaaatggcct gcgtgcagta gacggtggcg aacatgcccg ccgcccccag gccgaagctt
      841 gatagaccca accagttaga gcttataccc ttgatctcgt gccccccgct ctcgggccgt
      901 cttctggccg gtcctgagaa acccccgtgg cggccccctg ctcaccaccc gcgtccggct
      961 attctacccg tcatctccgg ccgccgggct tacccgctcg tgcccgtgac caccctgatg
     1021 gggcatggcc cgaacgggcc atcttctcgg ttgtcccgaa catggcccga acaggtgaag
     1081 tcggttctct ccgctcccgc ttgaatggaa cctacaagtt gcggatgtaa acaaaaaagg
     1141 agaacgaatc atgtacggca ctatcggaca cgaggtctcg cggcagcacc gatcggacat
     1201 tcggcgcgaa gtggcggcct accgcctcga gagcggtctg cggaccagcc acggcaaaga
     1261 gcacggatct ccggggtacc cggggcggaa gctcgcgtgg tatgcgctcc tcctcggcga
     1321 acgactccgc ggaccacaag gcgcggaata ccaccagtcg acagagaacg gaggtggaac
     1381 cgtgagctag acagaccgag atgacagcaa ctgtgtagca aacgcaacac ctgaaaaaga
     1441 ccaggaaagg aaccacaaac catgacgaag acgaaccact acctttacgc gctggcggca
     1501 ctgatgacgc tggtgatgct ggccgcgctg accgcgacca tgacgtccaa ccccgtccag
     1561 gcggccacca aggccaggac taccctgacc atccaggccg agggcgttga cctcttcggc
     1621 aaggtcaaga gcaccaggct cggctgcctc ggcaaccgca ccgtcaaggt ctacaagcag
     1681 aagggaaccg agcagagccc ctctaccgac cccgtgatcg cctccgacac ctcggagcgg
     1741 cagggcgacg tcggtgtgtg gtcggtgggg aacacgggga tggagggcaa gttctacgcc
     1801 cgcacccgcg ccacgctgaa ctgcaagggc gacacgagcc ccacgattct ggcccgcagg
     1861 taggcggcag aagccatcaa cggggcgccg gacatcccag ccggcgccct ttacacgaac
     1921 ttcaagagag gaatccagat gtttcatacg agagcaaacg cgatgctggg gctaaagggc
     1981 ctctttctcg ggcaactgat agcggtcttc atcatgctgc tcgccctcgc cacacccgcg
     2041 caggcgcagt tgacgtttcc ggtaacccgg accgacgatc ccagccccca ggggtgcttc
     2101 ccgggggact gttcgctccg ggaggcggtg atcgccgcca acggcacccc cggctccgac
     2161 accatagtgc tcgagccgac cacctacaac ctcaccatcg ccggtaatgg tggcgccgac
     2221 cagggcgacc tcgacatcac cgagaacctc gacatcgaga gcaccggacc aggcgacgcc
     2281 accataaacg ccaaccgggt cgaccgggtc ctcgaggtag tctccggtgg cctcagcctc
     2341 cgcgacatca ccgtccggaa cgggctcgcc accccggacg gccagggctt cttgcggggc
     2401 ggcgggatcc gggtgttcgg ctcgctctat ctctacgaca gcaaggttgt agataaccac
     2461 atgccaacgg taaacggagc cgacggcggc ggtatctaca acgacggcca caccgtgata
     2521 atgcgaagcg aggtgagcaa caacaccgca gttcaggggt tcggcggcgg catctacacg
     2581 ccggccaacg gggtcaccga gatccgcgac tcgaagctct ccggcaacga ggccgtgttc
     2641 gggggcgccc tcgcctcagc cagcggaggt ggcaacgtta tggtcgagcg cagccagctc
     2701 agcctcaaca cggccggcct gggtggcgcc tcttaccagc tcgggtcaag cacctacacc
     2761 ttcaccaaca ccacgctcga cggcaacacg gccaccgacg gtactggagg aggcgcgatc
     2821 cgggttcgta gtggcagcgc gacgctgaac aactcgaccg tgacccgcaa tgaggccccc
     2881 aacggcggcg ggctcgccgc cgtgaacgac gggagctcga tcactatcgc gaactcgatc
     2941 gtcgcgggca acaccgacac caaccaggca gacggcagct tccccgactg cct
//
LOCUS       DBNGVD010000428         7485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5021374, whole genome shotgun sequence.
ACCESSION   DBNGVD010000428 DBNGVD010000000
VERSION     DBNGVD010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5021374"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="AC16CM_11495"
     CDS             <1..746
                     /locus_tag="AC16CM_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3140500.1"
                     /translation="NVGGGRCPVVDTWWQTETGAIMIAPLPGLTATKPGSATRPFPGI
                     FVDIYDEEDNPIEGAGKGNLVITKPWPSMLRTIYKDPERFRETYWERLGDKYFVEDGA
                     ERDENGYYTITGRIDDVMNVSGHRIGTMEVESALVSHEGVAEAAVIGRHDEDKGQAIV
                     AYVILEGDSEGSDELIQELNQQVRKAIGPHARPEEIIFSPDLPKTRSGKIMRRVLRGV
                     AEGEDDLGDTSTLADPSVVDDLKEARRQS"
     gene            974..1669
                     /locus_tag="AC16CM_11500"
     CDS             974..1669
                     /locus_tag="AC16CM_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR3140501.1"
                     /translation="MPVGFGGYLLIMVVGMLISGAAALWVRSSYSKFSKQRSASGLTG
                     AQTARMILDRNGLSNVRVEPVAGQLTDHYDPRSKVVRLSEDNYTKNSIAGVSVAAHEV
                     GHAIQDASGYVPMKLRAGIFPVVNISSQFWPMAFIFAFFVGGAATPLGSLLINVAVIL
                     FVGVLAFHLVTLPVEINASTRAYGLLTQYGILSSSEAAGTRRVLTAAAFTYIAAALTA
                     VLTFLYLFLASRQ"
     gene            1780..2400
                     /locus_tag="AC16CM_11505"
     CDS             1780..2400
                     /locus_tag="AC16CM_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140502.1"
                     /translation="MEDVRRINNRRRRLRTVGEPHPWEPRHMRLSAMRFAESVGKPEE
                     RRNLTLGVVQHLWRSAVPFLAFYPPEGVRVTENRLYTPAPPKSVDDTDYLAGVEAHKR
                     AFAQDLEDAMVPAGHLPGYFEIPTEEGWRITLDLVGGRLRLADAWGLNSERRVAGDLA
                     PFVWAYRNCGFCLVTIYDKDIGAVRDDLLFVAKRVGVELRWRRTAA"
     gene            2537..3265
                     /locus_tag="AC16CM_11510"
     CDS             2537..3265
                     /locus_tag="AC16CM_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3140503.1"
                     /translation="MLTSHSTGGFATTTAPEGFGLEEFERAGLRTVERRFGPKDTIFT
                     PGDPDDQLYFLLSGTVRLYKIYGDYKEATTALLKDGGVFGKLNLVEGRWQDVFAEAVT
                     EARVASIQKSSIERVIKNDPSFALKLFSSLSERLRQSDEVIESLLHREVSTRLATLLL
                     NLSERFGEANGVGTLIDVRLTHQDLANMIASTREAVSKVMSEFQRDGLIESRNRRVVI
                     VDGDALSEYASRLTGLSIDGQAAI"
     gene            3418..4029
                     /locus_tag="AC16CM_11515"
     CDS             3418..4029
                     /locus_tag="AC16CM_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3140504.1"
                     /translation="MEYTSTVTLGGWREKLGSLSVRRAKLDDKELVTRTLAGDLRSYE
                     ELVSRYERLVGRVVYPYARREISVEDLVQETFLRAYDRLETFNPDYRFKTWLLAIANN
                     LGVDTLRRRREIVEFNPETHASLSHGPEAEAVDADRARGVKEAIATLPETYGVPLILR
                     YTEGLSYAEIAEVLSISVPAVKSRLFRARNMLAGRLEEAGERE"
     gene            4026..4529
                     /locus_tag="AC16CM_11520"
     CDS             4026..4529
                     /locus_tag="AC16CM_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3140505.1"
                     /translation="MSLSRRCDPEPIFELADGSLGVERVREVRAHLGDCEACRSLYER
                     ELQLNASLGSLDLEPSKSVCPGVAMALPTRSFRARAMWAVLAVVLLLTASAAMLLEGS
                     NLAVLPIQTAGMFLGFVASMADMVQVVFAVLGPALLVALAVGAVVDLAIAAVYLTVYR
                     RRRAREA"
     gene            4544..5524
                     /locus_tag="AC16CM_11525"
     CDS             4544..5524
                     /locus_tag="AC16CM_11525"
                     /inference="COORDINATES: protein motif:HMM:NF053377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8173 domain-containing protein"
                     /protein_id="HIR3140506.1"
                     /translation="MTGINTRSLVAVCIMVLGLIILMPLPAWADEQRLVGNIVVVGTE
                     DEVRTVAGNVTVQGLVEDDVQSGKGDITVSPGGSVRGDVDATLGNVNIQGPVGEDVNA
                     AFGKVYINAPVGGDVDVEHGDVELGPEAEISGDLTYGDGKFTGDSSAVKGDFSASGMS
                     FGLDESHGTSVLGFLGWLFASLAFAACAVLAAVVAPRSLAAAARRVGETPGRAFVYGI
                     VSLPVFFLLCVVLAVTIIGIPLILLLAPAYLAVLFAGTLVASFFLGTRVLMFTGRYRV
                     GNAMAAVVGALILAATTFIPLLGDLILYALCLLGTGAVIMAIFRVRRYEL"
     gene            complement(5597..6166)
                     /locus_tag="AC16CM_11530"
     CDS             complement(5597..6166)
                     /locus_tag="AC16CM_11530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140507.1"
                     /translation="MKPPILVDFPESFETERLLLRSPLPGDGPELHAAIRESRDDLAP
                     WMAWLTEHKTIADSEASTRRARVAFLARSELRLHVYLKGTDTLVGIAGLQGIDWDVPK
                     FEIGYWCRTRFTGSGYITEAVKAITAIAIDTLGARRVEIRCDPRNHKSARVAERAGFR
                     LEATLHNNDLGADGTVRDTLIFSRLGGDE"
     gene            6421..>7485
                     /locus_tag="AC16CM_11535"
     CDS             6421..>7485
                     /locus_tag="AC16CM_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3140508.1"
                     /translation="MRIEEMLKSGSGVRRAPGMSRKEFLRLGGAGVAGAALLSTAGCG
                     VFESGGGGGGGGGSTKTVAFNLEDAVRDLDSATTTDSVSTDILLNVMSGLYRLDVNTR
                     PVPDMAESVDISEDQLSYTFKIRDGVKWSNGDPVTAHDFEYAWKRVLNPDTGAQYAYI
                     ITTFVKGAAEYNEGDGSADDVAVKATDDKTLEVELLAPSPFWLGLTSFFTYLPQNQKF
                     VEQQGEDYALNKDALIFNGPYVLSDFKPTQGATMTKSDDYWNKANVPVQKAEGKIVKE
                     LDTAVNLYESGELDVQGIEGEYVEEYKDSPEYQTVTYFATFYMVGNQKEEVFQNLNIR
                     KAIQIGYDRDALVNQILKNGS"
BASE COUNT         1379 a         2332 c         2394 g         1380 t
ORIGIN      
        1 agaacgtcgg cgggggcagg tgcccggtcg tggacacgtg gtggcagacg gagaccggcg
       61 cgatcatgat agcgccccta cccggcctga cggcgaccaa gccaggcagc gccacccgtc
      121 ccttccctgg catcttcgtc gacatctacg acgaggagga caaccctata gagggtgcgg
      181 gcaagggcaa cctggtcatc accaagccgt ggccaagcat gctccggacc atctacaagg
      241 accccgagcg cttccgcgag acctactggg agaggctcgg cgacaagtac ttcgtcgagg
      301 acggggccga gcgcgacgag aacggctact acaccatcac gggccggata gacgatgtta
      361 tgaacgtctc cgggcaccgc atcggcacga tggaggtcga gagcgcgctc gtctcccatg
      421 agggcgtggc cgaggcagcc gtcatcgggc gccacgatga ggacaagggt caggccatcg
      481 tggcctacgt catcctcgaa ggtgacagcg agggctccga cgagctgata caggagctca
      541 accagcaggt caggaaggca ataggccccc acgccaggcc agaggagatc atcttcagcc
      601 cggaccttcc aaagaccagg agcggcaaga tcatgcgccg cgtcctcagg ggcgtcgcag
      661 aaggggagga cgatctggga gatacctcca ccctcgccga cccctccgtg gtcgacgacc
      721 tgaaagaggc ccgtcgccag tcgtaacctc tggataacca gaagaacggc ggaaacaggc
      781 ggggcgccag tcccgcctgt ttcttgcggt aaacggggcc ttttcgggcg atactgggta
      841 tcgtgccgca tgcagaccct ggtgcctgaa atctgtagcc cgaaacccgt attcgtgcaa
      901 ccttggggac ccgtctgcgt atgtacgggt gtagtaaaat atgtggcagt aaatctgcta
      961 ggaggaaaac aaattgccag ttggattcgg cggttacctg ttaattatgg tcgtcggtat
     1021 gctcatctcg ggggccgcgg ccctgtgggt caggagctct tactccaagt tctcgaagca
     1081 gaggagcgcc agcgggctca ccggcgccca gacggccagg atgatactcg accgtaacgg
     1141 cttgtcgaac gtcagggtcg agcccgtggc cgggcagctc acggaccact acgacccgcg
     1201 aagcaaggtc gtcaggctct ccgaggacaa ctacacgaag aactccatcg ccggcgtcag
     1261 cgtggcggcc cacgaggtgg gacacgccat ccaggacgcc agcggctacg tgcccatgaa
     1321 gctcagggcc ggcatcttcc cggtcgtgaa catcagcagc cagttctggc cgatggcctt
     1381 catcttcgcc ttcttcgtag gtggtgcggc gacgcccctg ggttcgctcc tgatcaacgt
     1441 cgccgtcatc ctgttcgtgg gtgttctggc cttccacctc gtgacgcttc cggtggagat
     1501 caacgcctct acccgcgcct acgggctcct gacgcagtac gggatcctgt ccagctcgga
     1561 ggccgctggc acgaggcggg tcctgaccgc tgcggctttc acctacatcg cggcggccct
     1621 gacggcggtg ctcaccttcc tctacctgtt cctggcaagc aggcaataaa gagccatcag
     1681 ctgatcgcca gctgacagct gaaatcgaaa ggcggggctc cggccccgcc tttttcatgc
     1741 tgggcaacgc ccgctataga gttgtagaat acgcgcgtca tggaagatgt tcgtcgcata
     1801 aacaatcgcc ggcgcaggct caggaccgtg ggagagccgc atccctggga gccgaggcac
     1861 atgcgtctct ctgcgatgcg cttcgccgag tcagtgggaa agccggagga acggcgcaac
     1921 ttgacgctcg gggtagtaca gcacctctgg cgctctgccg tacctttcct ggcgttctat
     1981 cctcccgagg gcgtaagggt cacagagaac aggttgtaca cgccagcccc cccgaagtcg
     2041 gtggacgaca cggactacct cgccggggtg gaggcgcaca agagggcctt cgcccaggat
     2101 ctggaggacg ccatggtccc cgctgggcac ctgccggggt acttcgagat tcccacggag
     2161 gaggggtggc ggatcaccct cgacctcgtt ggtgggcggc tgcggctggc tgacgcctgg
     2221 ggcctcaatt ccgagaggag ggtggccgga gacctcgccc cgttcgtgtg ggcctaccgc
     2281 aactgcggtt tctgtctggt caccatctac gacaaggaca tcggcgccgt tcgcgatgat
     2341 cttctcttcg tcgccaagcg cgtcggcgtc gagctcaggt ggcgcaggac cgccgcctga
     2401 acaccacgac gggcgccgcc gcggccgcct ttacacgaac ttttatcaag aatagttatt
     2461 ggaatctcgg gtataatgat cctattcggg aagtttcgcc ggttctgcag gaagtttctt
     2521 agagaggtcg gaacagatgc ttacgagcca tagcaccggg gggttcgcta ccacaaccgc
     2581 tcctgagggg ttcggtctcg aagaattcga gcgggccggc ctcaggaccg tggagcggcg
     2641 ttttggcccc aaggatacga tctttacgcc gggggacccg gacgaccagc tttacttcct
     2701 gctctccggc acggtgcgcc tgtacaagat ctacggcgac tacaaggagg ccacgacggc
     2761 gctcctgaag gacggcggcg tcttcggcaa gctgaacctc gtcgaggggc gctggcagga
     2821 cgtgttcgcc gaggccgtaa ccgaggccag ggtagccagc attcagaaga gctccataga
     2881 gagggtcatc aagaacgacc ccagcttcgc cctaaagctg ttctcctctc tctccgagag
     2941 gctcaggcag tccgacgagg tcatagagag cctgctccac agggaggtgt cgaccaggct
     3001 ggccacgctg cttttgaact tgagcgagcg cttcggcgag gccaacgggg ttggaaccct
     3061 catagacgtg cgtctcacgc accaggacct cgccaacatg atcgcctcca ccagggaggc
     3121 cgtctcgaag gtgatgagcg agttccagcg cgacggcctg atcgagagcc gcaaccgcag
     3181 ggtggtcatc gtggacggcg acgccctctc cgaatacgcc tcgcgcctga ctggcctgag
     3241 catagacggc caggccgcga tctagataag cattctgcct gcggcagctt atcagcacgc
     3301 tcggcctcga aggcctcgct tgtcgggggt ttgtggttct agcgtgccag gggcggctca
     3361 cgtggtcgat gattccccgg aaactttgtg gcacggcggg cgtatactcg tactagtatg
     3421 gagtacacat ctacggtgac attaggcggc tggcgcgaga agctcgggag cctctcggtc
     3481 cggcgcgcga aactggacga caaggagctc gtcacccgca cgctcgccgg ggatctgcgc
     3541 tcttacgagg agctggtgag ccgctacgag cggctggtcg ggcgtgtcgt atacccctac
     3601 gcgcggcgtg agatctccgt cgaagacctc gtacaggaga cgttcttgcg tgcctacgac
     3661 cgcctcgaga ccttcaaccc ggactaccgg ttcaagacct ggttgctcgc catcgccaac
     3721 aacctcggcg tggacaccct caggcgcagg cgtgagatcg ttgagttcaa ccccgagacc
     3781 cacgcgtccc tctcccacgg ccctgaggcc gaggcagtcg acgctgatcg cgccaggggc
     3841 gtcaaggagg ccatagccac ccttcccgag acctacgggg tgcccctgat cctgcgctac
     3901 accgagggcc tgagctacgc ggagatagcg gaggtccttt ctatctccgt gccggccgtg
     3961 aagagccggc tcttccgcgc ccgcaacatg ctggcgggaa ggctcgaaga ggcgggtgag
     4021 agggaatgag cctttcccgt cggtgcgacc cggaacccat cttcgagctc gccgacggct
     4081 ccctgggagt ggagagagtg cgtgaggtca gggcgcatct gggggattgt gaggcctgcc
     4141 ggagcctgta cgagagggag ctgcaactca acgcctccct cgggtcactg gatctcgaac
     4201 cgtcaaagtc tgtctgcccg ggcgtggcga tggcgctgcc aacccggtcc ttcagggcga
     4261 gggcgatgtg ggccgtgctc gccgtcgtgc tgcttctgac cgcctcagcg gccatgttgc
     4321 tcgagggatc caacctcgca gtcctcccta tacagacggc cggcatgttc ctgggcttcg
     4381 tggccagcat ggcagacatg gtgcaggtcg tcttcgcggt cctcggccct gcgctcctcg
     4441 tggccctcgc cgtaggcgcc gtcgtggatc tcgctatcgc cgccgtgtac ctgacggtat
     4501 accgtcggcg gcgcgccaga gaggcatgag ctactctcca tccttgacgg gaatcaacac
     4561 gcggtcgctg gtggccgttt gcatcatggt tctcggcctc atcatcctca tgcccctacc
     4621 ggcgtgggcc gatgagcaga ggctcgtcgg caacatcgtg gtcgttggga cggaggacga
     4681 agtacggacc gttgccggca acgtgacggt acagggcctt gtcgaagacg acgtgcaatc
     4741 cggaaagggc gacatcaccg ttagcccagg tggcagcgtg cggggcgatg tggatgccac
     4801 tctcggcaac gtgaacatcc aggggcccgt tggtgaggac gttaacgccg cgttcggcaa
     4861 ggtgtacatc aacgcccctg tcggcgggga cgtcgacgtg gagcacgggg acgtggagtt
     4921 gggcccggag gccgagatct cgggcgacct cacttacggc gacggcaagt tcaccggcga
     4981 cagcagcgcc gtgaagggcg acttcagcgc cagtgggatg tcgttcggtc tggatgagtc
     5041 tcacggtaca agcgttctcg gcttcttggg gtggctgttc gcctcgctcg ccttcgccgc
     5101 ctgcgcggtg cttgccgccg tggtagcgcc gcgctcgctc gcagcggcgg cgcggcgggt
     5161 cggcgagacg ccggggaggg cgttcgttta cggtatcgta tctttgcccg tcttcttctt
     5221 gctctgcgtc gtgctcgccg tgaccatcat cgggataccg ctcatcctgt tgcttgcgcc
     5281 ggcctacctg gcagtcctct tcgcagggac gctggtggca tctttcttcc tgggcacgag
     5341 ggtgctgatg ttcacaggcc gctacagggt tggcaacgcc atggccgccg tggttggtgc
     5401 cctcatcctc gccgctacca ccttcatccc cctccttggg gacctgatcc tctacgccct
     5461 ctgcctcctc ggaaccggcg ccgtgataat ggcgattttc agggtcaggc gctacgagtt
     5521 gtgagccgca acggaatatc gaagcggatg aaggcacctg ttagttcttc tctgtagcct
     5581 gtagccccga agcggcctat tcgtcaccgc cgagccgcga aaagatgagc gtatccctga
     5641 ccgtaccgtc ggcgccgagg tcgttgttgt gcagggttgc ctcgagtctg aatccggctc
     5701 gttcggcgac cctcgcgctc ttgtggttgc gggggtcgca tctgatctcg acccgtcttg
     5761 cgccaagggt gtcgatggcg atggcggtta ttgcctttac cgcttcggtg atgtagccgc
     5821 tgcccgtgaa gcgggttcgg caccagtagc cgatctcgaa cttcggcacg tcccagtcta
     5881 tgccctgcaa gcccgcgata cccaccagcg tgtccgtccc cttcagatac acgtgcagcc
     5941 tgagctccga acgcgccaga aaggccaccc gggccctcct ggtgctggcc tccgaatcgg
     6001 cgatggtctt gtgttcggtc agccacgcca tccagggtgc gagatcatct ctggactccc
     6061 tgatggccgc gtgtagttcg ggaccgtcgc cggggagggg gcttctgagc agcagccgct
     6121 ccgtctcgaa gctctcggga aaatccacga gtatgggagg cttcatgtct tgctccccgt
     6181 ggtcgcctgc ctcctattct agacctcgtt tggccgcgac gcccccgttt gtacatctgt
     6241 ataaccccgc cgggccgcga aattgcacgt tggtcgaagt tgcgcttata ctgcgcaggt
     6301 ataaggtttc aggaacaagg tgccttcagg accatcgagg cttcgtataa tggttggaga
     6361 ccatataatg ggagttcgag agtcccgggc cacgcgcgcc gggctaggaa aggagagtca
     6421 atgcggatcg aggagatgct gaagtcgggg agcggcgtgc gccgggctcc cggcatgagt
     6481 cgtaaagagt ttctgaggct gggcggcgcc ggggtggccg gggcggcctt gctatccacc
     6541 gccggctgcg gcgtcttcga gagcggtggc ggcggcggtg gcggcggcgg aagcacgaag
     6601 accgttgcct tcaacctgga agatgccgta cgggacctcg actcggcgac cacaaccgac
     6661 tccgtctcca ccgacatcct gctgaacgtg atgagcggcc tttacaggct agacgtgaac
     6721 acgcggcccg tgcctgacat ggcagagagc gttgatatta gcgaggatca gctcagctac
     6781 accttcaaga tacgcgacgg cgtcaagtgg tccaacggcg accccgttac cgcccacgac
     6841 ttcgagtacg cctggaagcg cgtcttgaac ccagacacgg gtgcccagta cgcctacatc
     6901 atcaccacct tcgtcaaggg cgcggccgag tacaacgagg gtgacgggag cgccgatgac
     6961 gttgcggtaa aggccacgga cgacaagacg ctcgaggtgg agctactcgc cccctcgccg
     7021 ttctggctcg gcctgacctc gttctttacc tacctgccgc agaaccagaa gttcgtcgag
     7081 cagcagggcg aggactacgc cctgaacaag gacgccctca tcttcaacgg accatacgtc
     7141 ctttccgact tcaagccgac ccagggcgcg acgatgacca agagcgacga ttactggaac
     7201 aaggccaatg tgcccgtcca gaaggcggag ggcaagatcg tcaaagagct cgacaccgcc
     7261 gtaaacctct acgagtccgg cgagctcgac gtgcagggca tcgagggcga gtacgtcgag
     7321 gagtacaagg actcccccga gtaccagacc gttacctact tcgccacctt ctacatggtc
     7381 ggcaaccaga aagaggaagt cttccagaac ctcaacatcc gcaaggccat ccagataggc
     7441 tacgaccggg acgcgctggt aaaccagatc ctcaagaacg gctcc
//
LOCUS       DBNGVD010000429         8242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_468440, whole genome shotgun sequence.
ACCESSION   DBNGVD010000429 DBNGVD010000000
VERSION     DBNGVD010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_468440"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="AC16CM_11540"
     CDS             <1..1197
                     /locus_tag="AC16CM_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR3140509.1"
                     /translation="GLSTNRRAINPRTVFGALAIQILIGVIALYWQAGRRALEAASNA
                     VQAVIDSSNEGIEFLFGPVLPEEGLVFAFQVLPVIIFFAALTSVLYHWGVLQWIVKLL
                     GGALQFVLRTSKAESTFAASTIFVGMSEAPLVVKPYIARATRSELFTMMGTGLATVAG
                     SLLVGYSLLGVRLEYLLAAAFMAGPAGIVMSKIIVPETGQPETLVASNVDEEAEEAEE
                     EDEEDDEEGHLNTIDAIASGTLQGLQIALTVGAMLIAFISLIALVNIILGAAGGLFGF
                     PDLTFELILGYVFAPLAFLIGVPWQEAVSAGSFLGQKLVLNEFVAFSNFGPQVEQFSQ
                     KTVAIVTFGLTGFANFGSIAILIGTFGSISPSRRHEVARFGIRAVLAGTLANLMNATI
                     AGILIG"
     gene            1207..2370
                     /locus_tag="AC16CM_11545"
     CDS             1207..2370
                     /locus_tag="AC16CM_11545"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR3140510.1"
                     /translation="MGSRATIVVLDGLGAGHAPDAAEFGDEGSNTLGNTAKAVGGLEA
                     PNLQALGFGNIGEIEGVAPTESPIASHGLMVERSAAKATLAGHWEMMGLVLEDPLPTY
                     PEGFPEEILRRFERETGRGVLGNRPASGTQIIEELGPEQERTGDWILYTSADSVFQVA
                     AHTGLIPLEELYEACGQAHEMLIGEGEIMVERVIARPFHGEPGAYVRENENRHDYGIT
                     PPSETYLDRIERVGHATVAVGKIRDIFDGKGLTEHLPAPPDDSAKVDAILEALGRVES
                     GLIFANLVDFDAKYGHRRDPEGMAGNIKRFDDRLPELLDAITEEDLLIITADHGNDPT
                     FSGTDHTRERVPLLIVGTGPRDLGVSQGFSDVGATVAAWLDVDKGGLPGKSLL"
     gene            2421..3353
                     /locus_tag="AC16CM_11550"
     CDS             2421..3353
                     /locus_tag="AC16CM_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140511.1"
                     /translation="METRRSDLTPDLFRPLDASARDNERIVGRPIGFWEDSWSRLKKN
                     RGALLSLALIILLFVMAFLLGPLLSGHSPFAQDMTRTYVDPSGEFWFGTDNFGRDMWT
                     RVWAGTRVSLYIAFLAAALDLFVGVPFGAISGFVGGRVDGVMQRIIEILNGIPNLVVA
                     ILAMVIFQPGILTISIAIGLTGWTYMARIVRGKMLQLKDQEFALASRSLGAGKFRLVW
                     RHLIPNSLGPIIISVMFTIPDAIFAESFLSFIGLGIQIPEASLGSLISDGTEYLQFHP
                     YLLWFPTAILCLIMISFTLFGDGLRDALDPKMRK"
     gene            3521..4795
                     /locus_tag="AC16CM_11555"
     CDS             3521..4795
                     /locus_tag="AC16CM_11555"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR3140512.1"
                     /translation="MQSVNAVIEAIETKKFGGELSEDIIRLVVEGYTAGEIPDYQMSS
                     LLMAIFIRGMHYAETLALTRAMAESGQTYSFPECVDKHSTGGVGDKVSLTALPIVAAC
                     GAPVAKLSGRGLGTTGGTIDKLESIPGFSFDLSEERFARQVEEVGLAIVEAGDLAPAD
                     KEIYALRDTTGTVDSLPLIGSSIVSKKVATGAGHLLYDVKRGSGAFMKTTEEARELAG
                     LLVRLSEDLNVKASALITDMDNPLGSAVGNALEVRESVSLLKNEPVPGDLTDEVNEVA
                     TRLLELKGIPDAAAVVEKALTSGAAYEKFRQFVGAQGGDVDALEDLPVSTEVREVKAP
                     AEGYVARFAASDIGSAALALGAGRARKDDEIDPGAGIEVLVRAGDHVRQDQPIARLYG
                     EHEAERARRLVLEALELSDEPVERPPAILGSL"
     gene            4886..5599
                     /locus_tag="AC16CM_11560"
     CDS             4886..5599
                     /locus_tag="AC16CM_11560"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HIR3140513.1"
                     /translation="MSPIHVRAEPGEFAESVLLPGDPLRAKYIAENFFEGAKLVTEER
                     GMLGYTGTYKGEPVSVQATGMGCPSASIVAEELIQLGAKNLLRVGTCGGYSEDLRLGD
                     LIVATSATPNDGTVSSLTGGIPYAPAAHFDVVHAAYHAGEGAGRRTFVGPIVSSDLFY
                     DPVEAPERVWGNLGVLAVEMEASAIFTIAAMHGVRAGCLLTVSDTIGAELVRISDEEL
                     RGAVDNMMELALDTLNALN"
     gene            5655..6983
                     /locus_tag="AC16CM_11565"
     CDS             5655..6983
                     /locus_tag="AC16CM_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HIR3140514.1"
                     /translation="MTILNESGIPDVLPPLHAVGNLARRLGDALVVVAGTQAEAHLVQ
                     SLTAASSPNLYPAGHDPHVVFAILDPEDPPPQGSLASRIVESVAGMRNVNLVLLVAAR
                     TAEALGVDATFEARLASRKLDLPVQMVDPEAPHEIPGTLSTDLDDRVVSALIELCPKR
                     RTSDLVETSAPKRGGLLGGLLGLGRDTPEQGDSRPVVLMGGTPSPDAARGLAAELSRA
                     GVEVAGAVPADGVADLPPLGEGTVVAVMDPNLATAAAAAERRGATVVKTLMPTGVDGT
                     ARFVQDVSEAAGAGTSEVVRARSAWEELGALRNRIRGKRIFFAGDTGFEVPLARFLAD
                     AGAVVLEVGAPRLDRRFLAAELQTLGPDVDVVVSPDHRGQANRIDEARPDLVVCSPGL
                     RLPLVARGHLCRSTQDFVRAGLYGYGGARRILELLASTLRRAETLDSLSL"
     gene            6980..>8242
                     /locus_tag="AC16CM_11570"
     CDS             6980..>8242
                     /locus_tag="AC16CM_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HIR3140515.1"
                     /translation="MRLVHGVYEGPQSYGILRTAASIRGVHTVFRALPGEAYFPTLYG
                     SRERTGELAPVTLSPLREQPDDSMAPGNVSRDLSGVLRRHPRAEIIVVARSEASLLSG
                     EETPSLPETGTRNPELVTFSWEAVGRKETEGAELALEDLVRVRGQDPPDRSPYPTVNL
                     FGPPVLSPGAAAEYAEIERLLDLIGIEVNARVPLGADARDLRRLTRAWANLLLYREVG
                     ESATLYLQDEHGIPRVTTPMIGAAGTGAALRAVGELCQLDPRSVQRTIWAELARTAKL
                     PWYARLEPPETFENRKVAIFGDFTYPLGLGYALAREVGLEVVACGTYLTHLERDFFFH
                     AHSFTEGAFAEDDPEAVAARIEAAKPDLIIGTHLEEGVAESLEVPFLPLCPPTADRPF
                     VERPLMGYAGSTVLADLLDGALREKKRKP"
BASE COUNT         1443 a         2718 c         2719 g         1362 t
ORIGIN      
        1 ggactctcga cgaatcgacg ggcgataaac ccgcgcaccg tgttcggtgc gctggcgatc
       61 cagatcctga tcggcgtcat cgccctgtac tggcaggcgg gaaggcgcgc gctggaggcg
      121 gcttccaacg ccgtgcaggc ggtcatagac tcctctaacg aggggataga attcctcttc
      181 ggccccgttc tcccggagga ggggctcgtc ttcgcgttcc aggtgttgcc ggtaatcatc
      241 ttcttcgccg ccctgacctc ggtgctctac cactgggggg tcctgcagtg gatcgtgaaa
      301 ctgctcggcg gggcgctgca gttcgtgctg cgcacgagca aggccgagtc cacgttcgcc
      361 gcctccacca tcttcgtcgg gatgtccgag gcgccgctgg tcgtgaaacc ctacatagcc
      421 agggcgacca gatccgagct gtttaccatg atggggaccg ggctggcgac ggtggccggc
      481 tctctgctcg tcggctactc gctgctaggc gtgcggctcg aatacctgct cgcggcggcc
      541 ttcatggccg gcccggctgg catcgtgatg agcaagatca tcgtccccga gacgggccag
      601 ccggagacgc tggttgcatc caacgtggac gaggaggccg aagaggcgga ggaagaagac
      661 gaggaggacg acgaggaggg gcacctcaac accatcgacg ccatcgcatc gggcactttg
      721 caggggctac agatcgcgct caccgtcggg gccatgctca tagcgttcat ctccctgatc
      781 gcgctcgtaa acatcatact cggcgctgcg ggcgggctgt tcgggtttcc tgacctgacc
      841 ttcgagctga tcctgggcta cgtgttcgcc ccactcgcgt tcctgatcgg ggtgccctgg
      901 caagaggccg taagcgcagg tagctttcta ggacagaagc tggtcctcaa cgagttcgtg
      961 gccttctcca acttcggtcc acaggtcgag cagttctccc agaagacggt ggccatcgtt
     1021 actttcgggc ttacgggctt cgccaacttc ggctccatag ccatcctgat cggcaccttc
     1081 ggatccatct cgccgagccg caggcacgag gtcgcccgat tcggcatcag ggcggtcctc
     1141 gccggaacgc ttgccaacct gatgaacgcc accatagccg gcatcttgat cgggtaagga
     1201 ggtaacgtgg gaagtcgcgc aacgatagtg gtgctggacg ggcttggggc cggtcacgcc
     1261 cccgacgccg ccgagttcgg ggacgagggc tcgaacacgc tcggcaacac ggcgaaggcc
     1321 gttggcggcc tggaggcccc gaacctgcaa gccctgggct ttggcaacat cggggagatc
     1381 gagggcgtcg cgccaaccga atctcccatc gcctcccacg gcctgatggt cgagcgctcc
     1441 gccgccaagg ccaccctcgc cgggcactgg gagatgatgg ggctcgtgct tgaggatccc
     1501 ttgccgacct acccggaagg cttccccgaa gagatactgc gccgcttcga gagggagacg
     1561 gggcgcggag tcctcgggaa caggccagct tcgggaacgc agataataga agagctcggt
     1621 cccgagcagg agcggacggg ggattggatc ctctacacct cagccgactc ggtgttccag
     1681 gtggccgccc acaccggcct gatccccctg gaagagctct acgaagcctg cgggcaggcc
     1741 cacgagatgc tgatcggtga gggtgagatc atggtagagc gggtgatcgc ccgtcccttc
     1801 cacggtgagc cgggcgccta cgtgcgcgag aacgagaacc gccacgacta cggcatcaca
     1861 ccgccctccg aaacctacct ggaccgcatc gagagagtgg gccacgcgac cgtggcggtc
     1921 ggaaagatcc gggacatctt cgacgggaag ggcctcaccg aacacctccc agccccccca
     1981 gatgactcgg ccaaggtgga cgctatcctg gaagccctgg gccgcgtgga gtcgggcctc
     2041 atcttcgcca acctcgtcga tttcgacgcc aagtacggac accgccgtga ccccgagggc
     2101 atggccggga acataaagcg tttcgacgac cggctaccgg agctgctaga cgccatcaca
     2161 gaagaggacc tcctgatcat caccgccgac cacggcaacg acccgacttt ttcgggcacc
     2221 gaccacaccc gcgagcgcgt cccgctcctc atcgtcggca ccgggccgcg cgacctcggc
     2281 gtcagccagg gcttctccga tgtgggggcc accgtggcgg cgtggctcga cgtcgacaag
     2341 ggcggtctgc ccggaaagag ccttctttaa gggtgccttg ccagaagcac atccttacag
     2401 gaatagagta gacttgtcgc atggagacca gacggagcga cctcacccct gatctcttca
     2461 ggccgctcga cgcaagcgcc agggacaacg agaggatcgt gggccgcccg atagggttct
     2521 gggaggactc ctggtcgcgg ctcaagaaaa atcgtggggc gctgttgagc ctggcgctca
     2581 taatcttgct gttcgtcatg gccttcctgc tcggtccgct actatccggg cactcgccct
     2641 tcgcccagga catgacccgc acctacgtag atccgtcggg agaattctgg ttcggcacgg
     2701 acaacttcgg cagggacatg tggacgcgcg tgtgggctgg gaccagggtt tcgctctaca
     2761 tcgcgttcct cgcggcggcg ctcgacctct tcgtgggcgt ccctttcggg gcgatctccg
     2821 gtttcgtggg cggccgggtg gacggcgtca tgcagcgcat catcgagatc ctaaacggca
     2881 tccccaacct ggtcgtcgcc atcctcgcga tggtcatctt ccagcccggc attctcacca
     2941 taagcatcgc gatcgggctg accggctgga cgtacatggc caggatcgtg cgcggcaaga
     3001 tgttgcagct aaaggaccag gagttcgccc tcgcctcgcg ctcgctcggg gctggaaagt
     3061 tcaggctcgt gtggcggcac ctcataccca actcgctcgg acccataata atctcggtga
     3121 tgttcaccat acccgatgcc atcttcgccg agtcgttcct cagcttcatc gggctcggca
     3181 tccagatccc ggaggcctcg ctcgggtccc tgatcagcga cggcaccgag tacctgcagt
     3241 tccaccctta cctcctgtgg ttcccgaccg ctatcctgtg cctcatcatg atcagcttca
     3301 ccctcttcgg cgacggcctg cgcgacgccc tcgacccgaa gatgcgcaag tagaggagtc
     3361 tggcgaccag atcggtcgca ttcaaaggtg cctcgcgtgt agggtagctt cgcgaagcgc
     3421 caactcgtcc cgagaattat ctcagctcga ccatgtagca gaccctgccg acccgtgcac
     3481 ccgttagaat gtgacagggg cgatcgaaag gggggaccga atgcaatccg taaacgcggt
     3541 gatcgaggcc atcgagacca agaagttcgg cggggagctc tcggaagata tcatccgcct
     3601 cgtggtggag ggctacacgg cgggggagat ccccgactac cagatgtctt cgctgctgat
     3661 ggcgatcttt atcaggggca tgcactatgc ggagacgctc gcgcttaccc gggcgatggc
     3721 cgagtcgggc cagacctact ccttccccga gtgcgtggac aagcactcga cgggaggcgt
     3781 cggtgacaag gtaagcctta cggcgctccc gatcgtggcc gcctgcggtg cccccgtggc
     3841 caagctctcg ggtcgaggtc tcggcaccac cggcggcacc atcgacaagc tcgagtccat
     3901 ccccggtttc tctttcgacc tctccgaaga acgtttcgcg cggcaggtcg aagaggtggg
     3961 gctcgcaata gtcgaggccg gggatctcgc cccggcggac aaggagatct atgccctccg
     4021 ggacactact ggaacggttg attcgttgcc gctcataggc tcatcgatag tctccaagaa
     4081 ggtcgccacg ggggcgggcc acctcctcta cgacgtgaaa cggggctcgg gggccttcat
     4141 gaagaccaca gaagaggccc gtgaactggc agggctcctc gtacgcctga gcgaggacct
     4201 gaacgtaaag gcctcggccc tgataacgga catggacaac cccctgggta gcgccgttgg
     4261 caacgccctc gaagtccgcg agagcgtgag cctgctcaag aacgagccgg tccccgggga
     4321 cctaacggac gaggtgaacg aggtcgccac ccgcctgctc gagctaaaag gcatccccga
     4381 cgcggcggcc gtggtcgaga aagccctcac ctcgggcgcc gcctacgaga aattccgcca
     4441 gttcgtcggg gcccaggggg gcgacgtcga tgccctggaa gacctccccg tctcaaccga
     4501 ggtacgggaa gtaaaggccc cggcggaggg ctacgtggcc cgcttcgcgg cctcggatat
     4561 cggcagcgcg gccctggccc tcggggctgg ccgcgccagg aaagacgacg agatagaccc
     4621 tggcgccggt atagaggtcc tcgtaagggc cggagaccac gtcaggcaag accagcccat
     4681 cgcccgcctc tacggcgaac acgaggcgga gcgggcgagg cgcctcgtac tcgaggcgct
     4741 cgagctctcc gacgagcccg tagagcgtcc ccctgctatc ctgggtagct tgtgaaggta
     4801 gccgtcggct ttcagcgttc agctaacgaa gctgaacgct gaaagctact agctgatagc
     4861 ttgcgacgac cggagggagg agcaaatgag ccctatccac gtacgcgcgg agccggggga
     4921 gttcgcggaa agcgtgctct tgcccgggga tcccctgcgg gcgaagtaca ttgccgagaa
     4981 ctttttcgag ggcgcgaagc tggtaacgga ggaacgcggg atgctcgggt acacgggcac
     5041 ctacaagggg gagccggtct cggtccaggc cacgggcatg gggtgtccca gcgcatctat
     5101 agtggccgag gagctcattc agctcggcgc gaagaacctg ctcagggttg gaacctgcgg
     5161 cgggtacagc gaggatttga ggctcggtga cctcatcgtc gcgacctcgg caacgccgaa
     5221 cgacggtacg gtctcctcgc tcaccggggg catcccctac gcccctgcgg cccacttcga
     5281 cgtggtgcac gccgcctacc acgccggcga aggcgcgggg cgcagaacgt tcgtcggccc
     5341 catcgtgagc tcggatctgt tctacgatcc ggtcgaggcc cccgagcggg tgtggggcaa
     5401 cctcggggtg ctcgccgtgg agatggaggc ctccgcaatc ttcaccattg ccgcgatgca
     5461 cggcgtgagg gccgggtgtt tgttgaccgt ctccgacacc atcggggctg agttggtcag
     5521 aatcagcgac gaagagttgc ggggcgcggt ggacaatatg atggaactgg cgctcgacac
     5581 cctcaacgcc ttaaactaat accttctgtg gggacgaggc gcgagaacgg gcctccgcga
     5641 gataggaacg ttgaatgacg atcctcaacg agtcggggat accggacgtt ctcccacctt
     5701 tgcatgccgt cggtaacctg gcacgaaggc ttggggacgc gctcgtggtg gtcgcaggca
     5761 cgcaggcaga agcgcacctg gtccagtccc tgacggccgc gtcatcgccg aacctctacc
     5821 ccgccgggca cgacccacac gtggtcttcg ccatcctcga tcccgaggac ccacccccgc
     5881 agggatctct ggcctcccgt atcgtggaga gcgtggctgg gatgcgcaac gtcaacctgg
     5941 tgctgctggt tgcggcgcgt acggcggagg ccctcggggt agacgctacc ttcgaggcaa
     6001 ggctcgcctc gcgcaagctg gacctgcccg tccagatggt cgaccctgag gccccgcacg
     6061 agatccccgg caccctatca accgacctgg acgatagggt ggtctccgcc ctgatcgagc
     6121 tctgccccaa gcgtagaacc tcggatctgg tggagacctc cgcgccaaag cgcggcggcc
     6181 tgctcggagg ccttcttggc ctcggccgcg acacccccga gcagggcgat agccggcccg
     6241 tggtgctgat gggcggcacc ccctcaccgg atgccgcgcg gggccttgcg gccgagctgt
     6301 cgagggccgg ggtggaggtt gccggggccg tgccggcgga cggggtggcc gaccttccgc
     6361 cgctcgggga gggcacggtc gtggccgtga tggacccgaa cctggcgaca gcggccgcgg
     6421 ctgcagagag acgcggggcc acggtcgtaa aaaccctcat gcccacgggc gttgacggaa
     6481 cggcccgctt cgtgcaggac gtgtcggagg cggccggggc cggaaccagc gaggtcgtga
     6541 gggcgaggtc ggcgtgggag gaactcgggg ccctgcgcaa ccgcatcagg ggaaagagga
     6601 tcttcttcgc cggcgatacc ggcttcgagg ttccgctcgc ccgctttctg gccgacgccg
     6661 gggcggtggt gctcgaggtc ggggcgccac ggctggaccg caggttcctg gcagccgagc
     6721 tgcagacgct ggggcccgac gtggacgtgg tcgtctcgcc agaccacagg ggtcaggcga
     6781 accggataga tgaggccaga cccgacctgg tggtctgtag ccccggcctc cgcctgccgc
     6841 tcgtcgcccg cggccacctg tgccgctcca cccaggactt cgtacgggcg ggcctctacg
     6901 ggtacggtgg cgcacgacgt atcctggagc ttctggcgag caccctaagg cgagcagaga
     6961 ccctggactc gctgagcttg tgaggctggt tcacggcgtc tacgagggac cgcagagtta
     7021 cggaatcctg cgcaccgcag ccagcataag gggcgtgcac accgtgttca gggcgctacc
     7081 gggggaggca tacttcccca cgctctacgg ttcccgcgag cgaacgggag agctcgcgcc
     7141 ggtcacgcta agcccgcttc gcgagcagcc ggacgactcc atggcgccgg gcaacgtctc
     7201 ccgcgacctc tccggggtcc tcaggcgcca cccgagggcg gagatcattg tcgtggcccg
     7261 ctcggaggct tcgttgctct cgggggagga gaccccgtct ctgcccgaga ccggaaccag
     7321 gaaccccgag ctcgtgacct tctcctggga ggcggtcggc aggaaggaga ccgaaggcgc
     7381 ggagctcgcg ctggaagatc tggtccgcgt ccggggacag gacccacccg acaggagccc
     7441 ctacccaacg gtcaacctct tcgggcctcc agtcctgtcc cccggcgccg cggccgagta
     7501 cgccgagatc gagcgtctgc tcgacctgat cgggatagag gtcaacgcca gggtcccgct
     7561 gggggccgac gcgcgggacc tgcgccgcct cacccgcgcg tgggcaaacc tgctcctcta
     7621 cagggaggtc ggggaatcag cgacgctcta cttgcaggac gagcacggca tcccgcgtgt
     7681 aacgaccccg atgatcgggg cagcggggac gggtgcggcc ctgcgggccg tcggcgagct
     7741 ttgccagctc gaccccagat ccgtgcaacg cacgatctgg gccgagctgg cccgcacggc
     7801 gaagctcccc tggtacgccc gcctcgaacc gcccgagacc ttcgagaacc gcaaggtcgc
     7861 catcttcggg gacttcacct accccctcgg cctcggctac gccctggccc gcgaggtcgg
     7921 gctggaggtc gtcgcctgcg gcacctacct gacgcacctc gaacgagact tcttcttcca
     7981 cgcccactcc ttcacggagg gcgctttcgc ggaggatgac ccggaagcgg tcgccgcccg
     8041 tatcgaggcc gcgaagcccg acctcatcat cgggacgcac ctggaagagg gcgttgccga
     8101 gtccctcgag gtgcccttcc tccccctctg tcctcccacc gccgacaggc ccttcgtgga
     8161 gaggcccctg atgggctacg ccggctcgac cgtcctcgcc gacctcctgg atggtgcgct
     8221 ccgcgagaag aaacggaaac cc
//
LOCUS       DBNGVD010000430         3609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1560414, whole genome shotgun sequence.
ACCESSION   DBNGVD010000430 DBNGVD010000000
VERSION     DBNGVD010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1560414"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16CM_11575"
     CDS             <1..510
                     /locus_tag="AC16CM_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3140516.1"
                     /translation="DIVVNNSGATWGAPPAEMPLERFDQVLRVNVRGTFLMSQAAGRR
                     MIETERGGTIINISSVAGLVGGRPDYMQTVGYNSSKGAIISMTRDLATSWAQHGITVN
                     AIAPGWFPTRMSGGLIDKFETEMLEGIPLHRFGNPEDLKGVVIFLASPAAAYITGQTI
                     VVDGGATAW"
     gene            complement(843..2114)
                     /locus_tag="AC16CM_11580"
     CDS             complement(843..2114)
                     /locus_tag="AC16CM_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140517.1"
                     /translation="MNGDKTASPIVRSVELPGRVGLSYVEQGDPSGVPVVFLHAIADS
                     WRSFEPVLSSLPWSVHAFALTQRGHGDASRPAGGYRPRDFAMDLAAFMDVLQLEAAVL
                     VGGSSGGFVARRFAIDYPNRTLGLVFLGSPASLSDKPGVLEMWDSTFSRLKDPLPPDF
                     VREFVKGTLARPIPQAFFETVVQENLKVPARVWRATLKGLLEDDSLRDLCEIKAPTLI
                     VWGDRDTILPRSDQDLLEASITGSQLLVYPGAGHAFYWEEPERVASDLAAFIASPRRK
                     SDSSKGMSKMSVQQNKETFRRYVDEVWKSENLDFADEIFAEKYLSHQSDGTTLERGPE
                     DVKKFVMEYRSAFSDIENIAEDMIGEDDKVVNRWTLHVTHTGDFRGIPATNKRITITG
                     IGIFRFSEDGKVVESWDSLDQLGMLRQLGAV"
     gene            complement(2265..2630)
                     /locus_tag="AC16CM_11585"
     CDS             complement(2265..2630)
                     /locus_tag="AC16CM_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3140518.1"
                     /translation="MTEQIAPSLDFEAMRRAIEGSDYEALVSFYADDAELRLVNKDST
                     PSSPTVLRGKEEISEMLRDVCGRAMTHHVEDEVVGEDRVSFNEACEYPDGVKVLGATT
                     MQLRDGMIVRQTNIEAWDG"
     gene            3100..>3609
                     /locus_tag="AC16CM_11590"
     CDS             3100..>3609
                     /locus_tag="AC16CM_11590"
                     /inference="COORDINATES: protein motif:HMM:NF044766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3140519.1"
                     /translation="MTEQTTQTPEQDRDALVERLFGAVLGMSDLYMVYIGNRLGFYGA
                     LADHGPATAAELADATNTHERYVREWLEQQAVSGILGVSGGESDRRYSLPEGHAEVLA
                     EPDNPNYLAPFARMMAGMVRPLPEVIEAFRSGGGVPYSRYDADFCEGQAEMNGLQFDN
                     LLGSEWLPAV"
BASE COUNT          647 a         1168 c         1107 g          687 t
ORIGIN      
        1 gatatcgtgg tcaacaacag cggcgccacc tggggggcac cgccagcgga gatgcccctg
       61 gagcgcttcg accaggtgct cagggtcaac gtgcggggca cgttcctcat gtcccaggcc
      121 gcggggcggc gcatgatcga gaccgaacgg ggcggcacca tcatcaacat atcctccgtc
      181 gccgggctgg tcgggggccg tcccgattac atgcagaccg tgggctacaa ctcctccaag
      241 ggagccatca tctccatgac ccgcgacctg gccacgagct gggcgcagca cggcatcacc
      301 gtcaacgcca tagcccccgg ctggtttcca acccgcatga gcgggggcct gatcgacaaa
      361 ttcgagaccg agatgctgga gggcatccca cttcaccgct tcggtaaccc agaagacctc
      421 aaaggtgtcg taatctttct cgcctcgccc gccgccgcct acataacggg ccagaccatc
      481 gtggtcgacg gaggagccac agcctggtag gcgcggcgct ttgcgccgcg ccgcacgctt
      541 cggcttcgcc tgcgcttgtc agcacgccgg ctccggctta cgcctccgcc ggcttgtcag
      601 cccgcgtcgg ccttgcggcc tcgctttcag cacgctccct tcggctctcg cgttcagcca
      661 gtcgtctctc tccgtaacgg tggcttcgac tacccacctt taccgggcat cgaatcttca
      721 gaacggaagg gtgcaggtcg cagcgggatc tggctgacag cgagccaggc gcagccgggc
      781 ggagcgttgc tgacaagcga aggcgctagc ctgagcgtgc tgacaaggcg cgaagcgccg
      841 tgctagacag cgcctagctg tcgaagcatc cccaactggt cgaggctgtc ccagctttct
      901 acgactttgc cgtcttcgga gaagcggaag atgcctattc cggtgatcgt tatgcgcttg
      961 ttggtggcgg ggatacccct gaaatcgccg gtgtgcgtga cgtgcagcgt ccagcggttc
     1021 acgaccttgt cgtcttcccc gatcatgtct tcggcgatgt tctcgatgtc cgagaaggcg
     1081 gagcggtatt ccatgacgaa cttcttgacg tcttcgggac cgcgttccag ggtggtgccg
     1141 tcggactggt gggagaggta cttctcggcg aagatctcgt ccgcgaagtc gaggttctcg
     1201 ctcttccaga cctcatcgac gtaacgtctg aaggtctcct tgttttgctg gaccgacatc
     1261 ttgctcattc ctttcgatga atcgcttttt ctccttggac tcgcgatgaa agccgcgagg
     1321 tcggacgcaa ctcgttcggg ctcctcccaa tagaaagcgt gtcctgcgcc tggatacacc
     1381 agaagctgcg agcccgtgat cgatgcttcg aggagatctt gatcgcttcg tggaaggatc
     1441 gtatcccgat ccccccacac gatgagggtg ggagccttga tctcacagag gtctctcagg
     1501 gaatcgtctt ctagtaatcc tttgagcgtc gccctccaca cccgcgccgg aaccttcagg
     1561 ttctcctgca ctacggtttc gaagaatgct tgcggtatcg gtcgtgccaa cgttcccttc
     1621 acgaactcgc gcacgaagtc gggtggcaag ggatccttca gcctggagaa ggtcgagtcc
     1681 cacatctcca gcacgcccgg tttgtctgaa aggcttgcgg gtgagccaag aaacacgagc
     1741 ccgagggtgc gattagggta gtctatcgcg aagcgtcggg ccacgaagcc accactcgaa
     1801 ccgcccacga ggacggccgc ctcgagttgg agtacgtcca tgaaggcagc aagatccatc
     1861 gcaaaatctc ggggacggta gccccctgcg ggacgactcg catcgccatg tcccctttgc
     1921 gtcaaggcga acgcatgaac ggaccacggc aaactggaga gcaccggttc gaacgatcgc
     1981 caggagtctg cgatggcgtg caaaaaaacg acgggaacac ccgacgggtc tccctgttcg
     2041 acataggaaa ggccaacccg tccgggaagc tcgacggacc ttacgatggg gctggcggtc
     2101 ttatcgccgt tcatcctggt ttccgcaccc gttgccctga ctacttgccc atcccgtaca
     2161 gaacccggcg atctgctgtt gaccgtccat acggtagggg gagcgcgttc tggcgcagga
     2221 gcctgccgcc gacgcgctcc ccctaccggt catcacgtgc ttgtctaccc gtcccaggct
     2281 tctatgttgg tctggcggac gatcatgccg tcccgcaatt gcatcgtcgt ggcgccgagc
     2341 accttgaccc cgtccggata ctcgcacgcc tcgttgaagg aaacgcggtc ctcgccgacg
     2401 acctcatcct cgacgtggtg ggtcatcgcc cgcccgcaca cgtcccgcag catctcggag
     2461 atctcctcct tgccacgaag caccgtcggc gaactgggcg tggagtcctt gttgaccagg
     2521 cgtagctcgg cgtcgtcggc gtagaaggag acgagcgcct cgtagtcgct cccctctatg
     2581 gcacggcgca tcgcctcgaa gtcgaggctc ggggctatct gttcggtcat gcggtactta
     2641 cctcctcgct agcaggtcga atctctccgg caccgggccg gacgcaagtc catgcatcac
     2701 ctcctcgctt gttccctatg tcgcaagcaa cgccttatat cagaagcccg gtccgcgcac
     2761 atcccccaaa tgagtgatct tggagacagg gaagttgggc gagcatgacg gccaaaggac
     2821 tttttgcacg ggcccgcccc tggctcgtgg acggccggga gtgtggggcc gatcttgcat
     2881 gatcgccctt tcggccgaga actccctctt gttacactgt tcatgggatg cgtacggggc
     2941 tcccggaccg tgtcccgtgg aagagatgcg ggcccgagga acgcgggggc aacgcgtgag
     3001 gcggccgcag agggcggccg tggaccgaac ccccatcaac cggtaggggc ggttcgcaaa
     3061 ctgcccgatc gggaccggac tctcgaaagg agcaacgcca tgacggagca aacgactcaa
     3121 acccccgagc aagatcgcga cgcgctcgtg gagcgtctct ttggtgcggt tctcgggatg
     3181 agcgatctct acatggtcta catcgggaac cggcttggtt tctatggcgc gctcgccgac
     3241 catggacccg ccaccgcggc cgagctcgcc gacgcgacga acacgcacga acgctacgtg
     3301 cgggagtggc tggagcagca ggccgtgagc ggcatcctcg gggtctccgg cggggagtcc
     3361 gaccgccgct acagcctccc ggagggccat gccgaagtcc ttgccgagcc cgataaccct
     3421 aactacctgg cgcccttcgc caggatgatg gcgggcatgg tgaggccctt gcccgaggtc
     3481 atcgaggcct tccgttccgg aggcggggtc ccctactcgc gctacgacgc ggatttttgc
     3541 gagggccagg ccgagatgaa cgggctccag ttcgataacc tcctcggcag cgagtggctc
     3601 ccggccgtg
//
LOCUS       DBNGVD010000431         4299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1742619, whole genome shotgun sequence.
ACCESSION   DBNGVD010000431 DBNGVD010000000
VERSION     DBNGVD010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1742619"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="AC16CM_11595"
     CDS             <1..1094
                     /locus_tag="AC16CM_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR3140520.1"
                     /translation="LMVYDPTGALVASPTMGLPESIGGERNWDYRYTWLRDAALTLYA
                     LIYVGFEDEARNFMGWLRNRCQKDEDGLLQPLYGIDGRTDLTEVELSHLSGYKNSAPV
                     RLGNGAQHQRQLDLYGAVLDAAYLYNKYGAPLDYDLWKSLTKILDWLSDNWRDPDEGI
                     WEVRGGRRQFVSSKLLSWVALERAGRIARQRGLPGGNGRWERERDDIYEEIMEKGWNE
                     ERNSFVQFYGSDALDASLLMMPLVKFVGPTDPRWLATLDRIQQELTYDSLVDRYQTDS
                     AAPDGLEGEEGSFNLCSFWLVECLTQAGRIEEARLALEKMFSYSNHLGLYSEEIGYSG
                     EALGNFPQAFTHLALISAAVHLDRALGAR"
     gene            1095..2000
                     /gene="ftcD"
                     /locus_tag="AC16CM_11600"
     CDS             1095..2000
                     /gene="ftcD"
                     /locus_tag="AC16CM_11600"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR3140521.1"
                     /translation="MTLPAPLFEAVPNFSEGRDAAKISRIVEAVGGVPGIRVLGVHSD
                     PDHNRSVLTFAGEEEAVLSGAVALARSCAAELDLTSQAGEHPRMGVLDVLPFVPLAGA
                     TMEDAAGLARRAGEVLGSLGYVVFLYGAAASAPHRRDLPDIRRGGYEGVAERLSDPRW
                     RPDHGPRTLDLGRGAVAVGARPFLVAFNAFLDTDDVEVARTIARRIRESGGGLPALKA
                     LGLLVDGRAQVSMNLTDLSLTPIHLALEAVRSAASDLGASVESTELVGLAPLPAILDA
                     ARHYLGLRELGPEHVLETNLHDLSS"
     gene            2026..2505
                     /locus_tag="AC16CM_11605"
     CDS             2026..2505
                     /locus_tag="AC16CM_11605"
                     /inference="COORDINATES: protein motif:HMM:NF016874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIR3140522.1"
                     /translation="MPSPLFSKPERLFNLQLTARLEASGFRVFLPQRDGVDRKTAPYD
                     AIGSEDRRRAMFRLDRQKVLDCDVFLFLLDGRVPDEGACVELGIAHCERYLRGGGKLF
                     GGLHTDTRAAFIGSRLNPMVRVALDYITEDEQALLRLLSGYGTWRRNAEAERGGGNP"
     gene            2502..3341
                     /locus_tag="AC16CM_11610"
     CDS             2502..3341
                     /locus_tag="AC16CM_11610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3140523.1"
                     /translation="MNGRELTVAAVQMSSTPNKDENLTTAGRLIRAAAASGAELVALP
                     ELWSCHGLESAYHENAEPIPGETTNFLGNLARELGLYLLGGSILEEAPVSSKLHNTST
                     LFRPDGELAAVYRKIHLFDVKAPDREYLESGTIVPGEEIVTAKAGAATLGLSVCYDVR
                     FPELYRLLALRGAEVLMVPAAFTLQTGKDHWELLLRARAVENQAFVVAPAQWGQKADG
                     RWTYGRSLIVDPWGTVLAQCPDRDGFALATLDLGYLDRLRRDFPALANRRPAAYSWGE
                     TGE"
     gene            3345..3833
                     /locus_tag="AC16CM_11615"
     CDS             3345..3833
                     /locus_tag="AC16CM_11615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140524.1"
                     /translation="MSSEPFEVRVATAGDAPAVADLFYYTILHVNVGDYTASQVRAWA
                     GPAPDPELWKGRIDEHRGRRMFVASSDEEIVGFAELEEDGHLDTLYVHHEHQGRGVAS
                     ALLDRIEAEARRLGLERLYTEASITAKPFFRARGFSSVRPQLVELHGHSFRNFVMEKR
                     DI"
     gene            complement(3874..>4299)
                     /locus_tag="AC16CM_11620"
     CDS             complement(3874..>4299)
                     /locus_tag="AC16CM_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3140525.1"
                     /translation="QWLPTAGAVLTFVLAGEVVAGSGAAGLLAAGAESLGGLFPAFSP
                     MLAALGGALTGSNAASNALFMPLQVEAARGLGLSPDLTAAVQNVAGSQASMLSPQRLV
                     LAATATGLLGRESEILRTALLPVAVSVGLLAIVGMIVLP"
BASE COUNT          754 a         1410 c         1435 g          700 t
ORIGIN      
        1 agcttatggt ctacgacccc acaggggctc tcgtggcctc acccacgatg ggtttgccag
       61 agagcatcgg cggcgagagg aactgggact accgttacac ctggctcagg gacgcggcgc
      121 tgacgctcta cgccctgatc tacgtcggct tcgaagacga agcgcgcaac ttcatgggct
      181 ggctgaggaa ccgttgccag aaggacgagg acggccttct acagcccctg tacggtatcg
      241 acggacgcac cgacctgacc gaggtggagc tctcgcacct ctcagggtac aagaactccg
      301 cccccgtgcg gctcggcaat ggcgcgcagc accagaggca gctcgacctg tacggggctg
      361 tcctcgatgc cgcgtacctc tacaacaagt acggtgcccc gctcgactac gacctctgga
      421 agagcctcac caagattctg gactggttga gcgacaactg gagggacccc gacgagggca
      481 tctgggaggt gcggggaggg cgccgccagt tcgtctcctc caagctcctg agctgggtag
      541 ccctggagcg ggccggcagg atcgcccgcc agcgtggcct tcccgggggt aacggacggt
      601 gggagcggga acgcgacgat atctacgagg agatcatgga aaaaggctgg aacgaggagc
      661 ggaacagctt cgtccagttc tacggctcgg acgccctcga cgcgtcgctg ctgatgatgc
      721 cgctggtcaa gttcgtgggc cccacggacc cgcgctggtt ggcaacgcta gaccgcattc
      781 agcaggagct gacctacgac tccctggtcg accgctacca gacggatagc gcggcaccgg
      841 atggcctgga gggagaagag ggaagcttca acctgtgctc tttctggctc gtcgaatgcc
      901 tgacgcaggc cgggcgcatc gaggaggcgc gcctggccct ggagaagatg ttctcctact
      961 ccaaccacct cgggctgtac tccgaggaga tcggctactc cggcgaggca ctgggcaact
     1021 tcccgcaggc tttcacccac ctggccctca tcagcgccgc cgtccacctg gaccgcgccc
     1081 tcggcgcgcg ctgaatgacc ttgcctgccc cacttttcga ggcggtgccc aacttctcgg
     1141 aggggaggga cgcggcgaag atctcgcgca tcgtcgaggc ggttggcggg gtgccaggca
     1201 tcagggttct tggcgtccac tccgatcccg accacaaccg cagcgtgctt accttcgccg
     1261 gtgaggagga ggccgtgctc tcgggcgcgg tggccctggc acggtcgtgc gccgcggagc
     1321 tggatctgac ctcgcaggcg ggcgagcatc ccaggatggg cgtgctcgac gttcttccct
     1381 tcgttccgct cgctggcgcc acgatggaag acgcagccgg tctggcccgc agggctggcg
     1441 aagtcttggg ctcgctcggc tacgtcgtgt ttctgtatgg cgccgctgcg agcgccccgc
     1501 accgccgcga cctcccagat atcaggcggg ggggctacga gggcgttgca gagcgcctgt
     1561 cggacccgcg ctggagaccc gaccacgggc cacggacgct cgacctgggc aggggagccg
     1621 tagccgtcgg ggcccgcccc ttcctcgtgg cattcaacgc gttcctggac accgatgacg
     1681 tcgaggtagc ccgcaccatc gccaggcgca tcagggagag cggcggcggc ctccccgcgt
     1741 tgaaggcgct cggcttgctg gtagacggaa gggcccaggt ctccatgaac ctgaccgacc
     1801 tcagcctgac ccccatccac ctcgccctcg aagcggtaag gtcggccgcc tcagacctcg
     1861 gcgcgtccgt cgaatcgaca gagctcgtag gcctggcccc gctcccggcg atcctcgacg
     1921 ctgcccgtca ctacctgggc ctgcgggagc tcggcccgga acacgttctg gagacaaacc
     1981 tccatgacct atcctcctga aagccggcaa cgtcacgagc ccggagtgcc gagtccactg
     2041 ttctcgaagc cggagcggct cttcaacctc caactgaccg cgaggctgga ggcttcgggt
     2101 tttcgggtct tcctcccgca gcgtgatggc gtggatcgga agactgctcc ctatgacgct
     2161 attggctcag aagatcgccg tcgcgccatg tttcgactcg acaggcagaa ggttctggac
     2221 tgcgacgtct tcttgttctt gctcgacggg cgcgttccag acgagggggc atgcgtggag
     2281 ttgggcatcg cccactgcga gagatacctg cggggaggcg ggaaactctt cggcgggcta
     2341 catacggaca cccgcgctgc cttcatcggc tccaggctga acccgatggt gcgcgtggcg
     2401 ctagactaca taaccgagga cgagcaggcg ctgttgcggc tactatccgg atacggaact
     2461 tggaggcgga acgcagaggc ggaacgcgga ggaggaaacc catgaacgga cgtgagctga
     2521 cggtggcggc ggtccagatg tcttccaccc cgaacaagga tgagaacctg acgacggcgg
     2581 ggcgcctgat ccgggccgcc gcagcctccg gcgcggagct ggtggcgcta cccgagttgt
     2641 ggagctgcca cggcctcgag agcgcctacc acgagaacgc agaaccaatt cccggagaga
     2701 caaccaactt cctgggtaac ctggcaagag agctgggcct ctacctgcta ggtggttcca
     2761 tcctcgaaga ggcacccgta tcctcgaaac tgcataatac ctcgacgctt tttcggccgg
     2821 acggggagct ggctgccgta taccgcaaga tacatctctt cgacgtcaag gcccccgacc
     2881 gggagtatct ggagagcggg accatcgttc ctggcgagga gatcgtgacg gcgaaggcgg
     2941 gtgccgcgac gctcgggctc tcggtctgct acgacgtgcg ctttccggag ctctaccggc
     3001 tgctggcact gcggggtgcc gaggtgctta tggttccggc cgccttcacg ctccagaccg
     3061 gcaaggacca ctgggagctg ttgctgcggg cgcgggccgt ggagaaccag gccttcgtgg
     3121 tcgcgccggc ccagtgggga cagaaggccg acggccggtg gacctacggg cggtccctca
     3181 tcgtggaccc ctgggggaca gtactagccc agtgcccaga ccgcgacggt ttcgccctcg
     3241 ccaccctcga cctgggttac ctggaccgac tacgccggga cttccccgcc ctcgccaacc
     3301 gccgtcccgc ggcctatagc tggggagaga ccggggaata gacgatgagt tccgagccct
     3361 tcgaggtgag ggtggccacc gctggcgacg ccccggcagt tgccgacctc ttctactaca
     3421 ccatcctcca cgtgaacgtc ggggactata ctgcctctca ggtcagggcc tgggctgggc
     3481 ccgcgccgga tccggagctg tggaaagggc gcatcgacga gcatcgcggc cggaggatgt
     3541 tcgtggcctc ctccgacgag gagatcgtcg gcttcgcgga gctggaggag gatggacacc
     3601 tggacaccct gtacgttcac cacgagcatc agggccgcgg tgttgcctcg gcgttgctcg
     3661 acaggatcga ggcagaagcg cgccggctcg gactcgagcg actctacacg gaggcgagca
     3721 tcaccgccaa gcctttcttt cgggccagag ggttctcgtc cgtgaggccc cagctcgtcg
     3781 agctccatgg gcactccttc cgaaacttcg tcatggagaa gagagacatc tgacccttac
     3841 cgggtcgtgg ctcacgtaag acgcgttgcc gcactacgga agcactatca tgcccacgat
     3901 ggccagcagg ccaacggaga cggcgacggg aaggagcgcg gtacggagta tctcgctctc
     3961 ccggccaagc aggcccgtgg ccgtggcggc tagcaccagc ctctgggggg agagcatgct
     4021 ggcctggctc cccgccacgt tctgcactgc cgccgtgagg tcgggggata gcccaagacc
     4081 tcgcgcggcc tcgacctgta gcggcatgaa cagcgcgttg gaggcggcgt tggatcccgt
     4141 caaagcgccg cccaacgccg cgagcatggg tgagaacgca gggaagagcc cgccaaggga
     4201 ctccgccccc gcggccagga gaccagccgc gccgctcccg gcaacgactt cgccggccag
     4261 aacgaacgtc aggacggcac ctgcggtcgg tagccattg
//
LOCUS       DBNGVD010000432         2095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_832232, whole genome shotgun sequence.
ACCESSION   DBNGVD010000432 DBNGVD010000000
VERSION     DBNGVD010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_832232"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..340)
                     /locus_tag="AC16CM_11625"
     CDS             complement(92..340)
                     /locus_tag="AC16CM_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140526.1"
                     /translation="MSPGGEERADGRSGMAETVAKALGQIVGTLLFPFVVMLAIGGVY
                     YLIRRPRVTFWGAVFRWWVVLIAVVLLVLGLLGQVASA"
     gene            complement(337..708)
                     /locus_tag="AC16CM_11630"
     CDS             complement(337..708)
                     /locus_tag="AC16CM_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140527.1"
                     /translation="MLLRPASFFAAAGGRASYAGPIIYALACHAIAVLFAGAHDTICD
                     QDQCNDATVFRIPEEQERYEYGSPEYEALGDISARISEGLEEPQASRDEAADRASQLL
                     DQWSKMQEEYYRAFNEVYGVG"
     gene            complement(780..>2095)
                     /locus_tag="AC16CM_11635"
     CDS             complement(780..>2095)
                     /locus_tag="AC16CM_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3140528.1"
                     /translation="VSLFLLAVIVVVTLAAVWGFLSWRATTYLVTANSFRLKRGVFQK
                     SERTIPLDHVQSVDTVQGVIQRAFSVYEVRIETAGGGASEPDASLSALDRTAAQTLRR
                     EIQGSRTERVEQAEGPKVLRRLSTGDLLIAGATSGQIGVAFSLLAIGSQFFDDFLSDA
                     FVRRLIETLAPNWLMVLLIFVPVGLLLAWFLAIAGTVLAYSGFTLSREGDFLYIKRGL
                     LERREATIPLSRIQAIRVTEGVLRQPFGLAAVRVESAGYGEDAGVSTMLFPLLPHEEV
                     REFLAEATPEFAVNPTLQRLPSRALRRYIFRAMVGYLLLSIAVAVAWLVVFGSFLGLL
                     AFLLVPPSALYGWLSFRDSGWAFEEDRLVVRYRSLGRKTAIAPRRRLQSREVARSPFQ
                     RRLRLATFLAEVASGSGGSALRVTDLDAKTAEVLAGNLGPQGAGA"
BASE COUNT          386 a          682 c          716 g          311 t
ORIGIN      
        1 gcttgcaacc caaccatctc tctcacgttc ttccctccgc tcgccggcac cgatagcaga
       61 gtacgccgca gaggtccgtc ctggcgacgt ttcaggcgct cgcgacctgg cccaggaggc
      121 ccaacacgag caggaccacg gctatgagca cgacccacca gcggaaaacc gcgccccaga
      181 acgtcaccct cggacgccga atcaggtagt agacgccccc gatcgcgagc atgacgacga
      241 acgggaagag caaggttcca acgatctgtc ccagtgcttt cgccaccgtc tcggccatcc
      301 ccgatctccc gtcggccctc tcctcgccac cagggctcac ccgacaccgt acacctcgtt
      361 gaaggcgcgg tagtactctt cctgcatctt actccactgg tccagtagct gcgatgccct
      421 gtcggcagcc tcgtcgcggg aggcctgagg ttcttccagc ccctccgaga tgcgggcgga
      481 gatatctcca agcgcctcgt actcggggct tccgtactcg tatctctctt gctcctcggg
      541 gatccggaac acggtggcgt cgttacactg gtcctggtcg cagatggtgt cgtgggcgcc
      601 ggcgaagagc accgcgatgg cgtggcaggc cagggcgtag attatgggcc cggcgtacga
      661 ggctctgccg cctgcagccg cgaagaacga agcggggcgc agcagcacgt ctctggtggt
      721 ggcgacgaag gtagcggctg ggcttgcggg gtcgaaaccc gatggcgcgg gtttcgtggt
      781 caggccccgg cgccttgcgg tcccaggttg ccggccagga cctcggccgt cttcgcgtcg
      841 aggtcggtga cccgcagggc ggagccgccg gaaccggagg cgacctcggc gaggaaggtg
      901 gcgagccgca gacgacgctg gaaggggctg cgcgccacct cacgcgactg gagcctgcgc
      961 cggggtgcta tggcggtctt gcggcccaag cttcggtagc gcacgaccag ccggtcctcc
     1021 tcgaacgccc accccgagtc cctgaaggag agccatccgt agagtgccga gggtgggacc
     1081 aggaggaacg cgagaaggcc caggaacgac ccgaagacca cgagccaggc gaccgcgacg
     1141 gcgatggaga ggagcaagta tccgaccatg gccctgaaga tgtagcgtct gagggcccgg
     1201 ctgggaagtc tctgcagggt ggggttcacg gcgaactcgg gggtcgcctc ggccaggaac
     1261 tcccttacct cttcgtgggg caggaggggg aacagcatgg tcgagacgcc ggcgtcctca
     1321 ccgtagccgg cgctctccac gcgaacggcg gcgaggccga agggctggcg gagcacgccc
     1381 tcggtaacgc ggatggcctg gatacgggag agcgggatgg tcgcctcgcg gcgttccagg
     1441 aggccccgtt tgatgtacag gaaatcaccc tcgcgcgaga gcgtgaagcc ggaataggcc
     1501 agaaccgttc cggctatagc caggaaccag gcaaggagca ggccaacggg tacgaagata
     1561 aggagcacca tcagccagtt cggcgcgagg gtttcgatca ggcgccggac gaaggcgtcc
     1621 gagagaaaat cgtcgaagaa ctgggacccg atggccagca gggagaaggc taccccgatc
     1681 tggccgctcg tggcgcccgc gatcagcagg tcacccgtcg agagccgccg gagaaccttc
     1741 gggccttcgg cctgctcaac cctctccgtc ctggaaccct ggatctcgcg ccgtaacgtc
     1801 tgcgccgcgg tcctgtcgag cgcggagagc gaggcgtccg gttcgctggc gcccccgccg
     1861 gcggtctcga tccttacctc gtagaccgag aaggcgcgct ggatgacgcc ctggaccgtg
     1921 tcaaccgact ggacgtgatc caggggtatc gtacgctcgc tcttctgaaa gaccccccgc
     1981 ttgagccgga acgagttggc cgtcacaagg taggtggttg cccgccagga gaggaagccc
     2041 cagacggccg ccagggtgac gacgacgatc acggctagca ggaagaggga gaccg
//
LOCUS       DBNGVD010000433         6090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2107130, whole genome shotgun sequence.
ACCESSION   DBNGVD010000433 DBNGVD010000000
VERSION     DBNGVD010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2107130"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="gcvPA"
                     /locus_tag="AC16CM_11640"
     CDS             <1..1066
                     /gene="gcvPA"
                     /locus_tag="AC16CM_11640"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR3140529.1"
                     /translation="RIVPSAIGAIISRGEYLTCYTPYQPEISQGTLQAIFEFQSMISE
                     LTGLEISNASVYDGANAVVEAALMTARLTKRDPKVAVSAGLNPRYREVMETYRVEVVE
                     LPFGEGTTDFSAVPEDVSGVFVQSPNYFGIVEDVRAASEAAHEAEALAVAVCDPISLS
                     ILEAPGNLGADVAVGEAQPLGMPLMFGGPYAGYMATKENFVRQLPGRITGETIDEDGK
                     LAYVLTLRGREQDIRRARANSNICTNQALTALCATVYTALMGPRGLSEVAELSVSKAH
                     YLADRLQASGFRLRYPDAPFLWEFAVEVPDVARANEALLEAGMVGGLDLGDGAMLVAV
                     TEKRTREELDAFVEVVADAV"
     gene            1056..2465
                     /gene="gcvPB"
                     /locus_tag="AC16CM_11645"
     CDS             1056..2465
                     /gene="gcvPB"
                     /locus_tag="AC16CM_11645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3140530.1"
                     /translation="MPFDLIRDKGREGRRGYTLPKPDVEMKKLEDLLPKASLREEKPR
                     LPEVDEPTVIRHYTNLSRQNEGIDTTFYPLGSCTMKHNPRANEAAAVVPGFAGLHPLQ
                     PGTQGALRIMHELQGFLSEVSGLPHVSLQPLAGAQGELTSILMVKKFFEDAGDDGRTT
                     VIVPSTAHGTNPATASMAGFKAKEVGLDEHGCVEVEELREMVDGSTAALMITNPNTCG
                     VYERNIVEIAEILHEAGAFLYMDGANMNANMGRMRPAEADVDIMHFNLHKTFSTPHGG
                     GGPGCGAIACSDALAPFLPDPVVEKDEDAYRFVRPEKTIGRMSTFHGNFGQMLRAWNY
                     IRMHGPELRDVTDMAVLNANYVRAQLRGTYRIPYDELCKHEVVVQPPEGMRALDIAKG
                     LIDYGYHPPTIYFPLVVKEAMLIEPTETESKETLDDFVGTMLELAETSTEDLEEAPTH
                     APTRRLNETRAARQLKARW"
     gene            2468..3547
                     /gene="gcvT"
                     /locus_tag="AC16CM_11650"
     CDS             2468..3547
                     /gene="gcvT"
                     /locus_tag="AC16CM_11650"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3140531.1"
                     /translation="MGELKHTPLYEEHRASGARLVDFAGYEMPVQYEGIRAEHEAVRN
                     HAGLFDVSHMGEAVFRGPDAEAAVQRLVTRDVERLDIGQAGYCAVCYENGGTVDDVIV
                     YRREDDFLVVINASNREKDLEHFRTNTEDLDVEVADESDDWALLALQGPEAEGLLQDL
                     TDTDLSALRPFRFAEGEVEGVRAIISRTGYTGEDGFEIYLSPEGAPRVWRRLVDLGVS
                     PAGLGARDTLRLEAGMCLYGNELDAETTPLEAGIGFAVHLEKDPHFIGQRALLIEDTQ
                     GLRKKLVGFEMEGRGIARHGYHVTSGAEVVGEVTSGTKSPTLDQAIGLAFVDPGVDDW
                     FEILIRDNPVPAKAVPLPFYKRNRG"
     gene            3558..3935
                     /gene="gcvH"
                     /locus_tag="AC16CM_11655"
     CDS             3558..3935
                     /gene="gcvH"
                     /locus_tag="AC16CM_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019017580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3140532.1"
                     /translation="MAVPEDLQYTRSHEWVRSEDGTVTIGITEHAQEELGDVVFVELP
                     DVGSTLSAGESFGTVESVKAVSDLYAPVGGEVVEVNSSLEDAPEKINDDPYGEGWIVK
                     LNTSEEADLLSPDEYEKVVEEES"
     gene            complement(3963..4754)
                     /locus_tag="AC16CM_11660"
     CDS             complement(3963..4754)
                     /locus_tag="AC16CM_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3140533.1"
                     /translation="MSDLTTAEYSVGRVAALSGVTIRTLHHYDEIGLLSPGGHSQAGY
                     RLYGEADLRRLQRILFYRELGFALKEIQAIIDDPGTDHIGHLGRQRGLLVERIERLSA
                     MVDAIDKETEARKMNINLTPEERFEVFGDFHPEDHAEEAERRWGETDAYKESNRRVSR
                     YTKEDWLKLKAEAEEIEAQLAAAFEAELPPESDEAMAAAEAHRQHISRWFYHCTYEIQ
                     RGLAEMYVGDERFRSHYEERVPGLAYFVRDAVLANAERGDEDAVR"
     gene            complement(4890..5792)
                     /locus_tag="AC16CM_11665"
     CDS             complement(4890..5792)
                     /locus_tag="AC16CM_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140534.1"
                     /translation="MSQHEKVVPEAATPMLPPTLRLGAVHLTVTDLERSIPFYEDAIG
                     LRLHQRENSVVAMGVGGEDLLVLYEEPEARRAGRHAGLYHYALLFPSREELAHVALRL
                     AATRTRIQGASDHGTHEAIYLPDPDGNGIELAADRPREHWPQTMEYAGGPHPLDLDGL
                     LEVVAGEEPRDEAGPGLSIGHVHLHVGDLEQGLDFYRDVLGFEQTALIPGAAAFVAAG
                     GYHHHLGFNVWRGEGVPPVPEGRVGLRHWTVVLEDQEQVVEVGKRTLAAGIATEEHES
                     GFLVRDPWGIPVLFTAPDAPTGRE"
     gene            complement(5789..>6090)
                     /locus_tag="AC16CM_11670"
     CDS             complement(5789..>6090)
                     /locus_tag="AC16CM_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140535.1"
                     /translation="DITGPRSLGQDDVAALLSGVSGRPVEAVAVDDEAFVQGLAASGM
                     PEPAARSIAAFGRAIREGYISEAPGEVEKLTGRPPRSLLEVFEANRGDLMQGVST"
BASE COUNT         1076 a         2011 c         1994 g         1009 t
ORIGIN      
        1 caggatagtg ccctctgcca tcggcgcgat catctcccgc ggcgagtacc tgacctgcta
       61 cacgccatac cagccggaga tcagccaggg caccctgcag gccatcttcg agttccagtc
      121 catgatctcc gagctgacgg ggctggagat ctccaacgcc tccgtctacg acggcgccaa
      181 cgcagtggta gaggccgccc taatgaccgc gaggctgacg aagcgggatc cgaaggtcgc
      241 cgtctcggcc ggcctgaacc cccgctaccg cgaggtgatg gagacctacc gcgtggaagt
      301 ggtggagctg cccttcggag aagggaccac ggatttctca gcggttcccg aagacgtctc
      361 aggcgtcttc gtacagagcc ccaactactt cggcatcgtg gaagacgtga gggccgcctc
      421 cgaggcggcg cacgaggcgg aggccctcgc ggtcgccgtg tgcgatccga tctcgctctc
      481 catccttgag gcgcccggaa acctcggtgc cgacgtggcg gtgggcgagg cccagccgct
      541 cgggatgccc ctgatgttcg gcgggcccta cgccggctac atggctacga aagagaattt
      601 cgtccgtcag ctccccggac ggatcacagg ggagaccata gacgaggacg gcaagctggc
      661 ctacgttctc accctgcgcg gccgcgagca ggatatcagg cgcgccaggg ccaactccaa
      721 catctgcacc aaccaggcgc taacggccct ttgcgcgacc gtctacacgg ccctgatggg
      781 gccgcggggg ctgagcgagg tggccgagct ctccgtctcc aaagcacact atcttgccga
      841 taggctacag gcttcaggtt tcaggcttcg gtacccggac gctcctttct tgtgggagtt
      901 cgcggtggag gtgcccgacg tggcccgggc gaacgaggct ttgctcgaag cggggatggt
      961 tggcggactc gacctcgggg acggggccat gctcgtcgcc gtaacggaga agaggacaag
     1021 agaagagctc gacgccttcg tggaggtggt tgccgatgcc gtttgacctg atccgggaca
     1081 agggccgcga gggccgcagg ggctacaccc ttccgaagcc agacgtggag atgaagaagc
     1141 tcgaagacct cctgccgaaa gcgtcgctcc gcgaggagaa gcctcgcctt cctgaggtgg
     1201 acgagccaac cgtgatccgt cactacacca acctctcgcg ccagaacgag ggcatcgaca
     1261 caaccttcta cccgcttggc tcctgcacga tgaagcacaa cccacgcgcc aacgaggccg
     1321 ccgctgtggt tcccggtttt gccggtcttc acccgctcca gcccgggacg cagggcgcgc
     1381 tccgcatcat gcacgagcta cagggtttcc tgtccgaggt ctccgggttg ccccacgtct
     1441 cgttgcaacc gctcgccggc gcgcagggcg agttgacgag catcctgatg gtcaagaagt
     1501 tcttcgagga tgccggggac gacgggcgta ccaccgtcat cgttccatcg acggcgcacg
     1561 gaaccaaccc cgccaccgcc tcgatggccg gtttcaaggc aaaggaggtc gggctcgacg
     1621 agcacggctg cgtcgaggtg gaggagctgc gggagatggt ggacggctcg acggccgcgc
     1681 tcatgatcac caacccaaac acctgcggcg tctacgagcg aaacatagtg gagatagcgg
     1741 agattctgca cgaggccggc gccttcctgt atatggacgg cgcgaacatg aacgcgaaca
     1801 tgggacgcat gcgccccgcc gaggccgacg tggacatcat gcacttcaac ctgcacaaga
     1861 ccttctcgac cccccacggc ggcggaggcc ccggctgcgg ggccatcgcc tgctccgatg
     1921 ccctcgcgcc cttcctcccc gaccccgtcg tggagaagga cgaggacgcc taccgcttcg
     1981 tccgtccaga gaagaccatc gggcgcatgt ccacgttcca cggcaacttc gggcagatgc
     2041 tgagggcctg gaactacatc aggatgcacg gccccgagct gagggacgtg acggacatgg
     2101 ccgtcctgaa cgctaactac gttcgggctc agctgcgggg aacctaccgc atcccctacg
     2161 acgagctgtg caagcacgag gtcgtggtgc agccgcctga gggcatgaga gccctagata
     2221 tcgccaaggg gcttatcgac tacgggtacc atccgcccac catctacttc cccctcgtcg
     2281 tcaaggaggc catgctgata gagccgaccg agaccgagtc caaggagacc ctcgacgact
     2341 tcgtcgggac gatgctggag cttgccgaga cgagcacaga agacctcgaa gaggccccca
     2401 cgcacgcccc cacccgccgc ctcaacgaga cccgcgccgc ccgccagctc aaggccaggt
     2461 ggtaggggtg ggagaactca agcacacccc gctctacgaa gagcacaggg cctccggggc
     2521 caggctcgtg gacttcgccg gctacgagat gcccgtgcag tacgagggta taagggctga
     2581 gcatgaggcg gtgcgcaacc acgccgggct cttcgacgtg tcgcacatgg gcgaggccgt
     2641 gttccgcggc cccgatgccg aggccgctgt ccagcgcctc gtcacccgcg acgtggagag
     2701 gctggatatc gggcaggccg gctactgcgc ggtctgctac gagaacgggg ggacggtgga
     2761 cgacgtgatc gtctaccgcc gcgaggacga cttcctcgtc gtaataaacg cctccaaccg
     2821 cgagaaagac ctcgaacact tccgtacgaa caccgaggac ctcgatgtcg aggttgccga
     2881 cgagtccgac gactgggctc tgctggcatt gcaggggccc gaggccgagg ggctgctaca
     2941 ggacctgacc gacacggacc tctccgccct caggcccttc cggttcgccg aaggggaggt
     3001 agagggtgtc agggccataa tctcccgcac gggctacacg ggcgaggacg ggttcgagat
     3061 ctacctctcc ccagaaggcg ccccgcgcgt ctggcgccgg ctcgtagatc tcggggtcag
     3121 cccggccggg ctcggtgcca gggacacgct gcgcctcgag gccggcatgt gcctctacgg
     3181 caacgagctc gacgccgaga ccacgccgct tgaggccggt atcggcttcg cggtccatct
     3241 ggagaaggac ccgcacttca tcggccagcg ggcgctcctg atcgaggaca cccaggggct
     3301 gcgcaagaag ctcgtgggct tcgagatgga ggggcgaggc atagcccgcc acggctacca
     3361 cgtcaccagc ggggccgagg tggtcgggga ggtcaccagc ggcaccaagt cgccaacgct
     3421 ggaccaggcc ataggtcttg cgttcgtgga cccaggggtg gatgattggt tcgagatcct
     3481 gatcagggac aaccccgtgc ctgctaaggc cgtgccgcta ccattctaca aacgcaacag
     3541 aggatagggg gagagctttg gccgtaccgg aagatcttca gtacaccaga agccacgagt
     3601 gggttcgctc tgaggacggc accgtgacca taggcatcac ggagcatgcc caggaggagc
     3661 tcggcgacgt ggtcttcgtg gagttgcccg acgtgggttc caccctctcc gccggcgaat
     3721 ccttcggcac cgtggagagc gtcaaggcag tctcggacct gtacgcgcct gtcggcggcg
     3781 aggtcgtcga ggtcaactcc tcgctggagg atgcgccgga gaagatcaac gacgacccct
     3841 acggggaggg ctggatcgtc aaactgaaca cctccgaaga ggccgacctt ctctccccag
     3901 atgagtacga gaaggtcgta gaagaagagt cctagacgac cctggccggc ttgccagacg
     3961 gcttaccgga cggcatcctc atcgccacgc tccgcgttgg ccagcacggc atcccggacg
     4021 aagtaggcca ggccggggac ccgttcctcg tagtgcgagc gaaagcgctc gtcgcccacg
     4081 tacatctcgg cgaggccgcg ctggatctca taggtgcaat ggtagaacca gcggctgatg
     4141 tgctgccggt gggcctccgc cgctgccatg gcctcatcgc tctccggtgg gagttcggcc
     4201 tcgaaagctg cggcgagctg cgcttctatc tcctccgctt cggccttgag cttcagccag
     4261 tcttccttcg tgtagcgtga gacgcgccgg tttgactcct tgtaggcgtc ggtctcgccc
     4321 cagcgcctct cggcctcctc ggcgtggtcc tctgggtgga agtctccgaa gacctcgaag
     4381 cgctcttccg gtgtaaggtt gatgttcatc tttctcgcct ccgtctcctt gtctatagcg
     4441 tcgaccatcg cgctcagacg ctctatcctc tcgacgagca gcccgcgctg acgcccgagg
     4501 tgaccgatgt ggtctgtacc gggatcgtcg atgatggcct ggatctcctt caaggcgaaa
     4561 ccgagctccc tgtagaacag gatccgctgt agcctcctga ggtccgcctc accgtagagg
     4621 cggtagcccg cctggctgtg tccgccgggc gacagcaggc cgatctcgtc gtagtggtgc
     4681 aaagtccgga tcgttacccc ggagagggcc gccaccctcc cgacagaata ttctgctgtt
     4741 gtcaggtcgc tcacgtctca cctcctcgtc acagacaagt ctagaccctc acgttgcgtg
     4801 agggtcaagg cgattttctc gttcttctgc agactttccg gaccggagcc ctcaggctcg
     4861 tagtcagggg atgctgaccg ggatggggcc tattctcgtc ctgtgggggc atcaggggcg
     4921 gtgaagagga ccgggatccc ccacgggtca cgcacgagga agccgctctc gtgttcctcg
     4981 gtcgcgatgc cggcggcaag ggtgcgcttg cctacctcga ctacctgttc ctgatcttcg
     5041 agcaccacgg tccagtggcg caggcccacc cggccttccg ggacgggagg cacgccttct
     5101 ccccgccaga cgttgaaccc gaggtggtga tggtagcccc cggcggccac gaacgccgcg
     5161 gccccgggaa tcaaggccgt ttgctcgaac ccgagcacgt ctcgatagaa gtcgagcccc
     5221 tgctccagat ctccgacgtg caggtgcacg tggccgatgc tcagcccagg gccggcctca
     5281 tcgcgcggct cctcaccggc cacgacctcc agcaggccgt ccaggtcgag ggggtgcggg
     5341 ccgccggcgt actccatggt ctgtggccag tgctctcgcg ggcggtcggc ggcgagctcg
     5401 atgccgttgc cgtcggggtc gggaaggtag atggcctcgt gggtgccgtg gtctgaggca
     5461 ccctgtatcc gggtccgcgt ggcggccagg cgcaacgcta cgtgggcaag ctcctcgcgg
     5521 gaggggaaga gcagcgcgta gtggtagagg ccggcgtgcc tccccgcccg ccgcgcttcc
     5581 ggctcctcgt acaggacgag caggtcctcc ccaccgacgc ccatggcaac tacggaattc
     5641 tcgcgctggt gcagtcggag gccgatggcg tcctcgtaga aggggataga gcgttcgagg
     5701 tccgtgaccg tcagatgtac ggcgccaagg cggagcgtcg gtggcagcat cggcgtggcg
     5761 gcctccggga cgactttctc gtgctggctc atgtggatac cccctgcatc aggtctccgc
     5821 gattggcttc gaaaacctcc agaagcgacc gcggcggacg acccgtaagt ttctcgacct
     5881 ccccgggggc ctcgcttata tagccctcac ggatagcccg cccgaacgcg gcgatggagc
     5941 gggcggccgg ctcgggcatg ccggacgcgg cgaggccctg gacgaaagcc tcgtcgtcga
     6001 cggccacggc ctcgacgggc ctgcccgata ccccgctcag gagcgccgcc acgtcgtcct
     6061 ggcccagcga acggggcccg gtgatgtcgt
//
LOCUS       DBNGVD010000434         5712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4839196, whole genome shotgun sequence.
ACCESSION   DBNGVD010000434 DBNGVD010000000
VERSION     DBNGVD010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4839196"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC16CM_11675"
     CDS             <1..398
                     /locus_tag="AC16CM_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140536.1"
                     /translation="KDESQRNLEKWTEEEWQTREGEADAREDGETRRYLPKEAWENMS
                     EEEKEETERKKREGSKQGEQYVSNTDKAKKAREQAAAPPLNDYDDLSVEEVETKVKGL
                     SRGEIQDLLDYEKQHKNRKTLVEALGRRL"
     gene            complement(484..1272)
                     /locus_tag="AC16CM_11680"
     CDS             complement(484..1272)
                     /locus_tag="AC16CM_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3140537.1"
                     /translation="MDEQLNTLTGESIRFVELRSVWDKNVLDLTDDEIRRVGAAMSER
                     GIGVSSVGSPIGKVPIDENFAPHLERFRRALHVARVLESPYVRVFSFFMPEGEDPAAY
                     REEVLERMGVLATEAEDAGVVLVHENEKEIYGDVPSRCLDILTQVDSPYLRAAWDAAN
                     FVQCGVDHPYLEGYEALRPYVEYVHVKDALSGSGTVVPAGEGDGEIRETISALRASGF
                     EGFFSLEPHLASSGTYSGFSGPELFVRAARAFKNLLRQRNIPWS"
     gene            complement(1464..2546)
                     /locus_tag="AC16CM_11685"
     CDS             complement(1464..2546)
                     /locus_tag="AC16CM_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3140538.1"
                     /translation="MTPEKVRFGILGCGTIGPHHARAIAGLEEAELVAVADIVPEQAE
                     ELAAEYGCSSHGGLGEMLSGAGLDAVCVCTPSGMHAWDAIAALEAGKHVVIEKPVDVT
                     LGAADRILEAQRATGRKVAVVSQHRFDAATLAVQRAISRQQFGRLTMGSAEVRWWRSQ
                     SYYDSGGWRGTWELDGGGVLINQAIHSVDLLQCLMGRVVEVTAYTGMLAHERIEVEDT
                     AVAVLKFENGALGTIAATTAAYPGLTARIAVHGDRGSAVIDDDHLVYFHAAGMGQEPE
                     AYGGGENQAGRVMEHYGGSSTRPGAGADPGSLSMAHRDQIRDFIRAIKDDREPSITVE
                     EGRKPLAIIAGIYESARTGELVHIGR"
     gene            3006..3530
                     /locus_tag="AC16CM_11690"
     CDS             3006..3530
                     /locus_tag="AC16CM_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HIR3140539.1"
                     /translation="MATIGKSVSGRPDVECFSTFRDAIRDATNGRVSDAPKDASGFAG
                     DRAMQKRVFSGATEASAPGSRNISPRSVRGNDVVGCCNVLSVEYENRGFGGNTLTYRA
                     KGRPCDSDGGLEVGVSYVGDKWNDEISSYVGSGRCQVKHYQNSNYGGASTRLRQQDNY
                     MGIFNDETSSIKWG"
     gene            3736..5070
                     /gene="hisD"
                     /locus_tag="AC16CM_11695"
     CDS             3736..5070
                     /gene="hisD"
                     /locus_tag="AC16CM_11695"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3140540.1"
                     /translation="MSEAERRDPREGPRYLKRPSGERVKGSPPEIKNRVSEMLSRIER
                     GGLGAVREYSRELDGWDPPDFRVSEEEVGRAAGSVDPELREAIEFGRDNTRRFAEMQR
                     ETLVDFEREVAPGVVAGQRQVPVGSVGAYQPAGRVPLLASPFMTVAVPKVAGVERVIA
                     CVPPRREGGVHPAMLLSISVSGADAIYAVGGVQAMAAMAFGLFEDLEGPVDMIVGAGN
                     AYVAEAKRQLFGTVGIDLLAGPSEVAVIADASADPELVAADLLGQAEHGPTSPASLVT
                     TSGQLAEAVMEEVERQLADLETAEVAGAAWRDHGTVVVAADHGEAVLLMDEIAPEHLE
                     VHTEDDDWYLQNLRNYGSVFLGDRSTVAFSDKGMTGTNHVLPTAGAARYTGGLSVAKF
                     TKTLTYQRITGEEGTRRLAPPVMRFSREEGLWAHEATARKRLERLDGQVPDG"
     gene            5139..>5712
                     /locus_tag="AC16CM_11700"
     CDS             5139..>5712
                     /locus_tag="AC16CM_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3140541.1"
                     /translation="MTQNTGSQEQALQRTLGLSAVVIIGIAWVTPLIVLATFGIIAQL
                     SNGAAPTAYLITLVAMLFTAYSYGRMAYVHYQSAGSAYTYVGREMGPRLGFLTGWLIL
                     LDYFFIPLVVWLIGAAYLSAQFPEVPGAVWVLAFILITTALNIIGIRVTATANYLLVA
                     FQLLVLGIFVALSIGYLVGTDTGATLTSPFF"
BASE COUNT         1010 a         1812 c         1904 g          986 t
ORIGIN      
        1 agaaggacga gtcacagagg aacctcgaga agtggacaga ggaagagtgg cagaccagag
       61 agggcgaagc cgacgcccgc gaggacgggg agaccaggcg ctacctgccg aaggaagcct
      121 gggagaacat gagcgaggag gagaaggaag agaccgaacg caagaagcgt gagggctcga
      181 agcagggcga gcagtacgtc tccaacacag acaaggccaa aaaggcgcgc gagcaggccg
      241 ccgccccgcc gctcaacgac tacgacgacc tgagcgtcga agaggtagag acgaaggtca
      301 agggcctttc caggggcgag atccaggacc tcctcgacta cgagaagcag cacaagaacc
      361 gcaaaacgct ggtcgaagcg cttggccgca ggctatagca tcaggcgatt tcccgaagcc
      421 cgaaacctgt aatcttctat ccggcttcgc ttcctccgcg ggtgacgcgc gcgttctctg
      481 cgcctacgac cacgggatgt ttctctggcg gagcaggttc ttgaacgctc tggctgccct
      541 gacgaacagc tccgggccgc tgaagccgga gtaggttccc gaggaggcga gatgcggttc
      601 gagggagaag aaaccctcga acccggaggc gcgcagggcc gagatggtct cacggatctc
      661 accgtccccc tccccggcag gcacgaccgt gccggatccc gagagcgcat ccttgacgtg
      721 cacgtattcc acgtagggcc tcaacgcttc gtatccctcc agatacggat ggtccacccc
      781 gcactgcacg aagtttgcgg cgtcccacgc ggccctgagg tagggagagt cgacctgggt
      841 caggatgtcc aggcaacggc tcgggacgtc cccgtagatc tccttctcgt tctcgtgcac
      901 gagaacgacc cccgcgtctt cggcctccgt cgccaggacg cccatccgtt ctagcacctc
      961 ctccctgtag gcggcggggt cctccccttc cggcatgaag aaagagaaga cccgaacgta
     1021 cggggattcc agcacccgtg cgacgtgcaa cgcgcggcgg aacctctcca ggtgcggggc
     1081 gaagttctcg tcgatcggga ccttgccaat tggggagccg acggaggaga cgccgatacc
     1141 gcgctccgac atcgcggcgc cgaccctccg tatctcgtcg tcggtcaggt cgagcacgtt
     1201 cttgtcccag acgcttcgga gttccacgaa gcggatggat tcgccggtga gcgtgttcag
     1261 ctgctcatcc aactcgggag agatttcgtc cgcgaatccc gcaagggtcc acatttatgg
     1321 cgacctcctt gtcgcaacgg ggacgtaccg gcggtttgcc ttcgaagcct cgggcgccca
     1381 gcccgccgac gaggtggcgc tgaaggacca cgggaatgaa tatcggggac gcgtccctgc
     1441 catcgactct ccccggacgg ttttcacctt ccgatatgta cgagttcgcc ggtacgggcc
     1501 gattcgtaga tgccggcgat gatcgcgagc ggcttgcggc cttcttccac ggttatggat
     1561 ggctcgcggt cgtccttgat ggcccggatg aagtcccgga tctggtcccg gtgcgccatc
     1621 gagaggctgc cggggtcggc gccggcgccc ggtctcgtgg agcttccccc gtaatgctcc
     1681 atgacccgcc ccgcctggtt ctcaccaccc ccgtaagcct ccggctcctg tcccatcccg
     1741 gcggcgtgga agtacacgag atggtcgtcg tcgatcacgg ccgagcccct gtcaccgtgc
     1801 acggctatcc tggccgtgag gccggggtaa gcggcggtcg ttgccgcgat ggtgccgagg
     1861 gcgccgttct cgaacttgag gaccgccacg gctgtgtcct cgacctcgat tctctcgtgg
     1921 gcgagcatgc cggtgtaggc ggtgacctcg acgactcggc ccatcagaca ttgcaggagg
     1981 tcgacgctgt ggatggcctg gttgatcagg actccccctc cgtcgagctc ccacgtgccg
     2041 cgccacccgc ccgagtcgta ataggattgc gaacgccacc accgcacctc tgcagacccc
     2101 atcgtcaggc ggccgaattg ttgcctggag atcgcgcgct ggacggcaag ggtcgccgcg
     2161 tcgaaacggt gctggctgac gacggccacc ttccgacctg tcgcccgctg tgcttcgagg
     2221 atacggtccg ccgccccgag ggtgacgtcg actggctttt cgatgacgac gtgtttgccc
     2281 gcttctagag cggcgatggc gtcccaggcg tgcatgccgc tgggggtaca gacgcatacg
     2341 gcgtccagcc cagccccgga gagcatctct ccgagaccgc cgtgcgagga gcatccgtac
     2401 tctgcggcga gctcttctgc ctgctcgggt acgatgtcgg cgactgccac gagttcggcc
     2461 tcctcgaggc cggcgatggc ccgcgcgtgg tgcggtccta tggttccgca tccgaggatc
     2521 ccgaagcgca ccttctcagg cgtcatccat ccccccgtgc cggccgcagg cgctgtcgag
     2581 gggcacccca gaggccgtcc gccgcgagat gactgccttt ccggcctctt cggtgatgac
     2641 gattctgtcc atcctctccc tcggatcgtc cacgatacgc tcatgttatc gccctcacgg
     2701 gcggttcgcc gtcgcgtgtt cgaccagaag gccgtggcaa gagccggccc gagggatgag
     2761 agtagctagc cttgcgggcc acgggccgcc cggtcgatgc ctgagaaccg acactatgtt
     2821 atcttgcttt tgcggtagtg ttcggaccaa tcatgagggg ggaactggtg gaaaaacgtg
     2881 cgtccttgac cgttactttg ctagcagctt tgctttccgc cgtagcattg atcgccgtcg
     2941 tcggtgggca ggctcgagcc tcttcgaatg tcttggatgt tcgaggcgac agcggaaagc
     3001 attgtgtggc gacgatcggg aaatccgtct cgggccgtcc tgatgtcgag tgtttctcta
     3061 ctttccggga cgccatcagg gacgccacga acggccgcgt ttcggacgcc ccgaaggacg
     3121 cctccggttt cgcaggcgac cgcgccatgc agaagcgcgt cttctccggt gccacggagg
     3181 cctctgcccc aggctccagg aatatctctc caaggagcgt gaggggcaac gacgtggtcg
     3241 gctgctgtaa cgtcctgagc gtcgagtacg agaacagggg cttcgggggc aacaccctta
     3301 cgtacagggc gaaaggacgt ccctgtgaca gcgacggcgg cctcgaggtg ggcgtctcct
     3361 acgtcggcga caagtggaac gatgagatca gctcgtacgt gggatccggc cgctgccagg
     3421 tcaagcacta ccagaactcg aactacggtg gtgcatccac caggctcaga cagcaggaca
     3481 attacatggg catattcaac gacgagacga gctctatcaa gtggggatag gcttcagttc
     3541 ttggagctat cagcggtcag ctttcagcac gaagctggcc gctgaggacc gaccctccgc
     3601 ccccagggtc gacgcccggt gggctattcc acgggtgcgt gcgtgtggca ggatcacggg
     3661 gctaccgtgg tgcgtgtctg ggtatgaacc agggtgggat cgaagccacg ccagcgagat
     3721 gggagggagt ttgttttgag cgaggctgag aggcgagatc cgcgcgaagg cccccggtac
     3781 ctcaagcggc cctccgggga gcgggtgaag ggctcgccgc cggagatcaa gaatcgggtc
     3841 tcggagatgc tctcgcgcat cgagcgcggg ggcctggggg ccgtcaggga gtactcccga
     3901 gagctggatg gctgggaccc ccccgacttc cgcgtctccg aagaggaggt cgggcgggcc
     3961 gccggctccg tagatccgga gctgcgcgag gcgatcgagt tcggcaggga caacacgagg
     4021 cggttcgccg agatgcagcg cgagacgctc gtggacttcg agcgggaggt ggcgcccggc
     4081 gtcgtcgccg gccagaggca ggtccccgtc gggtcggtcg gcgcctacca gcccgccggg
     4141 agggtgccgc tcctcgcatc accgttcatg accgtggccg tgccaaaggt tgcaggtgtg
     4201 gagagggtga tcgcctgcgt gccgccacgg agggagggtg gggttcaccc ggcgatgctc
     4261 ctctccatct ccgtctctgg ggccgatgcc atctacgcgg tgggtggcgt tcaggccatg
     4321 gccgcgatgg ccttcgggct cttcgaggat ctggaagggc ccgtcgacat gatcgtgggc
     4381 gccggcaacg cctacgtggc cgaggccaag cgccagctct tcggtacggt tgggatagac
     4441 ctcctggccg ggccgtctga ggtcgccgtc atagccgacg cgtcggccga ccccgagctc
     4501 gtggcagccg acctgctggg ccaggccgag cacggtccga cctcgccagc ctccctggta
     4561 accacctccg ggcaactggc cgaggccgtc atggaggagg tagagcgcca gctcgcggac
     4621 ctcgagaccg ccgaggtggc cggcgccgcc tggcgcgatc acgggacggt ggtggtggcg
     4681 gcagaccacg gggaggcggt gctcctgatg gacgagatag cccccgaaca cctcgaggtc
     4741 cacacggaag atgacgactg gtacctgcag aacctgcgca actacggctc cgtctttctg
     4801 ggagacagat ccacggtggc cttctcggac aagggcatga cgggcaccaa ccacgtgctc
     4861 cccaccgcgg gagccgcacg ctacacgggc gggctctcgg tagccaagtt caccaagacc
     4921 ctcacctacc agaggataac cggagaagag ggtacgagga ggctagcgcc ccccgtcatg
     4981 agattctccc gggaagaggg cctctgggcc cacgaggcca cggcccgcaa gaggttagaa
     5041 cgcctggacg ggcaagtccc cgatgggtga gatcaggggc aacaggtatc aggctacagg
     5101 catcaggaat acctgaagcc cctatacaga ggaggaacat gacccagaat acgggaagcc
     5161 aagagcaggc tttgcaacgg actctggggc tcagcgccgt ggtcattatc gggatcgcgt
     5221 gggtaacgcc gctcatcgtg ctggccacct tcgggatcat cgcccagctc tccaacgggg
     5281 cagcgccaac ggcctacctc atcacgctcg tcgcgatgct ctttaccgcc tacagctacg
     5341 ggcggatggc gtacgtgcac taccagagcg cgggttccgc ctacacctac gtcgggcggg
     5401 agatggggcc gaggctcggc ttcctgaccg ggtggctcat cctgctcgac tacttcttca
     5461 taccgctggt cgtgtggttg atcggggcgg cctacctgtc ggcgcagttt ccggaggtcc
     5521 cgggggcggt ctgggtgttg gcctttatcc tgatcaccac ggccctcaac atcattggca
     5581 tcagggtgac ggccacggcg aactacctgc tggtggcctt ccagttgctg gtactaggca
     5641 tcttcgtcgc gctgagcatc gggtacctgg tcgggacgga caccggggcc accctgacga
     5701 gcccgttctt ca
//
LOCUS       DBNGVD010000435         6787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2107203, whole genome shotgun sequence.
ACCESSION   DBNGVD010000435 DBNGVD010000000
VERSION     DBNGVD010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2107203"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="AC16CM_11705"
     CDS             complement(<1..488)
                     /locus_tag="AC16CM_11705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3140542.1"
                     /translation="MLLDSLNPTQLDAVQHTEGPLLILAGAGSGKTRVLTHRVAHLID
                     QGLAAPEEVLAITFTNKAAREMKERVALLVGPDSRRMWVSTFHAFCARILRVHAEKLG
                     YKREFTIYDGADQVRLVKRCIVELGKDPKRFNPRSFQAQISSAKNVLMAPDDFLRNTE
                     GY"
     gene            complement(674..1468)
                     /locus_tag="AC16CM_11710"
     CDS             complement(674..1468)
                     /locus_tag="AC16CM_11710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140543.1"
                     /translation="MGRGPRSPGRGVRALQHSVPGRRRTRGARHPRANHRGPLTTPRP
                     AQDRLQAHLRGRTGLTESTVEVGHVTYRLTHPVSPDDLIDEEDFDRDERLPYWAELWP
                     SALALSRHLSTRELTGTRAIELGCGVGLPTIVALARGATVLATDHYEAALDFTAHNAS
                     TNLGREPHTALLDWRTPDIEDLGTFDLILAADVLYEHKNAVALADLVPRLLAPNGEAL
                     FADPRRDGAPGFLAAMQEGGFGDAVEEVTVEQRSRKVNVLLHHLRR"
     gene            complement(1351..1800)
                     /locus_tag="AC16CM_11715"
     CDS             complement(1351..1800)
                     /locus_tag="AC16CM_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3140544.1"
                     /translation="MRLTQKSKYAVRALMELALNERDCPLGVAELARRQRIPERFLEQ
                     IFGELRRANIVESRRGAHGGYRFAMPTEEISVLDIVEILDGEIRPARCSAGGVCYIAD
                     APLCATSKVWDEAREALEGVFGRYSIASLAAVEREERATHEPTTAGR"
     gene            complement(2000..2704)
                     /locus_tag="AC16CM_11720"
     CDS             complement(2000..2704)
                     /locus_tag="AC16CM_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:2000185 - regulation of phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex PhoU family protein"
                     /protein_id="HIR3140545.1"
                     /translation="MPRETFQQELDGLVSDLIELGGEVELSLENMVRAMETRDAEVAR
                     GELGVDLRYKARGMDIERECMILQARQAPVARDLRLLYTVQSVTNHLVRSGTLSEHIC
                     QAIAETADCERDEDLEIALVEMARTARNVFDEGLDVFRNNDLDRARDLQAKDDKVDLL
                     YSEAMNLIANPSEGGGGDPEWRMRAALMVHYLERISDHGVDVGGSTVFLVTGQRIEDA
                     MQQYLDRNVDHDGDAG"
     gene            complement(2708..3463)
                     /gene="pstB"
                     /locus_tag="AC16CM_11725"
     CDS             complement(2708..3463)
                     /gene="pstB"
                     /locus_tag="AC16CM_11725"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR3140546.1"
                     /translation="MKIENLSAFYGSFKAVSDVSMDIQANKVTALIGPSGCGKSTFIR
                     CLNRMHEVIPGARAEGHVRLGEQDIYAKASDPVIIRRRVGMVFQKPNPFSKSIYENVA
                     WGARINGYRGNMDELVERSLKHAALWDEVKDRLKESGHGLSGGQQQRLCIARTLAVEP
                     EVILMDEPASALDPVSTQKIEDTMEQLKREYTVVIVTHNMQQAARISDFTGFFFIENM
                     GDPGRLWEFDETEKMFSNPERKETEDYVTGRFG"
     gene            complement(3611..4534)
                     /gene="pstA"
                     /locus_tag="AC16CM_11730"
     CDS             complement(3611..4534)
                     /gene="pstA"
                     /locus_tag="AC16CM_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3140547.1"
                     /translation="MAQSSMKAGQQDGGPSGGDFKESLSTRYGLDRGFTVLVSVAAFV
                     GVAVLAVLLVDVTRDGSSMLSLEFLTSFPSQVFPENGGIYPALIGSLWLLGLTGAISV
                     PLGLGAAVYLEEYAGDTRINRMIEVNISNLAGVPSVIYGLLGLGIFVELLAPITGGGS
                     VLTGAMTLSLLILPVIIVATREALKAIPNSIREGGYALGATQWEVIWSHLLPRALPGA
                     ITGIILALSRAVGEAAPILVVGVSLYQTYVTTGPFDGYMALPTQIYDWISRPQEVFQN
                     SAAAGIVVAMVVLLVANSFAIWLRNRYQQRS"
     gene            complement(4534..5466)
                     /gene="pstC"
                     /locus_tag="AC16CM_11735"
     CDS             complement(4534..5466)
                     /gene="pstC"
                     /locus_tag="AC16CM_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3140548.1"
                     /translation="MAIRAEGTRSGAEAWKRNSRGRETRERVVKFFLMACAYLSIFTT
                     VGIVIVLFEETARFFLKVSPVEFLTSTQWAPPQGEFGVLPLVYGTLTATFIAIAVALP
                     LGLLTAIYLSEYAPRALRRWLKPALEILAGVPTVVYGFFALTFITPFFRDSVFSGIQS
                     FNALSAGLIMGVMIIPTVASVSEDAIYAVPLSLREGAYALGATRRETATRIVVPAALS
                     GIVAAVILGISRAIGETMIVTIAMGAQANWLGSPLEGMQAMTAYIVQVVGGETPRGSI
                     TYESIFAVGSALFLMTLALNLASYWFVRRYRETY"
     gene            complement(5562..6557)
                     /locus_tag="AC16CM_11740"
     CDS             complement(5562..6557)
                     /locus_tag="AC16CM_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3140549.1"
                     /translation="MWLCLVLGLSLGLVSCGSAGGRGDADMGNIRIEGSSTVYPITQA
                     AAELFREENPDARIEVGGAGTSDGFEAFCQGDTQISDASRPIEPEEIEVCEENNVEFI
                     EIPVAYDGISVVVNKKDNDWATDITSEELKKIWEPSAEGKVTEWSQVNPDWPNKPLDL
                     YGPGTESGTYEFFNEAIVANEEEVSRQSDVEMSEDDNVLVQGVSGDANALGYFGYSYY
                     ENNLDSLKALAVDGVKPSEDSIRNGEYLLSRPLFIYVSTNALENNKAVKPFVDFYLAP
                     ENLDRLVEAAKYVTMPASLEKEARAQYQDRTTGTVINADGQPKGGDLEKALKQSQ"
BASE COUNT         1218 a         2208 c         2086 g         1275 t
ORIGIN      
        1 atataacctt ccgtgttgcg caagaagtcg tcgggtgcca tgagcacgtt cttggccgag
       61 ctgatctggg cctgaaacga gcgggggttg aagcgcttgg ggtccttgcc gagctccacg
      121 atgcaccgtt tgacgaggcg gacctgatcg gcgccgtcgt agatggtgaa ctccctcttg
      181 tagcctagct tctcggcgtg gacgcgcagg atgcgggcgc aaaaggcgtg gaatgtggag
      241 acccacatcc tcctggagtc ggggccgacg agcagcgcca ccctctcctt catctccctg
      301 gcggccttgt tggtgaaggt tatagccagc acctcctcgg gggcggccag accctggtct
      361 atgaggtggg cgacgcgatg agtgagcacg cgcgtcttcc cgctgccagc cccggcaagg
      421 atgaggaggg ggccttccgt atgctgtacg gcgtcaagct gagtcgggtt taacgagtcc
      481 agaagcacag gtggtgagta taccagcagc catcccgcta tcctgccccc gaaacgccag
      541 ctgctctacc gtgaaggccg gccgcgagtc cccggatcaa gactcccctg cgggcaaaac
      601 cgtcacgccg gtataagcgt ggagccacct tcctgtgctc gtgccggtat gcagtctgcg
      661 cccaggaaac ggtctaccgc cgcaggtgat ggagaagtac gttcaccttt ctactgcgct
      721 gctcgaccgt aacctcttcg acggcgtccc cgaagccacc ttcttgcatg gccgctagaa
      781 aaccgggcgc tccatcgcgt cgggggtcgg cgaagagggc ctccccgttg ggggcgagca
      841 gcctgggcac caggtcggcg agagcgacgg cgttcttgtg ctcgtagagc acgtcggcgg
      901 cgaggataag gtcgaaggtt cccaggtcct ctatatccgg cgtacgccag tcgaggaggg
      961 ccgtatgggg ttcgcggccc aggttggtgc tggcgttgtg ggcggtgaaa tccagggcag
     1021 cttcgtagtg gtccgtggcc agtaccgtgg caccgcgggc gagggctacg atggtgggca
     1081 acccgacgcc acacccgagc tcgatggcgc gggtaccggt gagttcgcgc gtcgagaggt
     1141 ggcgcgatag ggcgagggcg ctgggccaca gttccgccca gtaggggagc cgctcgtcgc
     1201 ggtcgaagtc ttcctcgtcg atcaggtcgt caggcgaaac ggggtgcgtg agccggtaag
     1261 ttacgtggcc aacctcgacc gtcgactcgg tcaggcccgt gcggccacgc aggtgggctt
     1321 ggagcctatc ctgggccggc ctgggggtcg ttagcggccc gcggtggttg gctcgtgggt
     1381 ggcgcgctcc tcgcgttcga cggcggccag ggacgctatg ctgtagcgcc cgaacacccc
     1441 ttccagggct tcgcgggcct cgtcccagac cttgctagtg gcgcacaggg gggcgtcggc
     1501 gatgtagcag accccgccgg ccgagcatcg ggccggacgg atctcaccgt ccagtatctc
     1561 cacgatgtcg aggacggaga tctcctccgt cggcatcgcg aagcggtatc cgccgtgcgc
     1621 gccgcgacgg ctctcgacga tgttggcccg cctgagctcg ccgaagatct gctccagaaa
     1681 cctctcgggg atgcgctggc gccgggccaa ctcggccacc ccgagcgggc agtcgcgctc
     1741 gttgagcgcg agctccatca acgcccttac cgcgtacttg ctcttctgag ttagcctcac
     1801 gaaagatcac ccgaccaaca agaaacatct cgccaagaag attgtagcag agatagacaa
     1861 accacaaccc ctgaaaaggc gctccgaagc ccccgccatc gagaaccacc acggaaagat
     1921 gagctattcg agaggatgct tcaggaaatc tacctgtagc cttcgcgctt ctcggcgtta
     1981 ccctctttcg cggactatcc tagccagcat ccccgtcgtg gtcaacgttg cgatcgaggt
     2041 attgttgcat ggcatcttct attcgttgcc cggtaacgag gaacacggtg cttccgccaa
     2101 catctacccc gtggtcggag atacgttcca ggtaatggac catcagggcg gcccgcatgc
     2161 gccactccgg gtcgccgccg ccgccctccg aggggttggc gataaggttc atggcctcgg
     2221 agtacagcag gtccacctta tcgtccttgg cctgcaggtc gcgggccctg tccaggtcgt
     2281 tgttcctgaa gacgtcgagg ccctcgtcga acacgttgcg cgccgttctt gccatctcca
     2341 cgagggcgat ctccaggtcc tcgtcccgct cgcagtcggc ggtctccgct atcgcctggc
     2401 agatgtgctc gctcagggtg ccggaccgga ccaggtggtt tgtcacggat tgcacggtgt
     2461 aaaggagcct caggtcgcgg gccacggggg cctggcgggc ctgcagtatc atgcactccc
     2521 gctcgatgtc catgccgcgg gccttgtagc gcaggtccac cccgagctcc ccgcgcgcca
     2581 cctccgcgtc gcgggtctcc atagccctca ccatgttctc cagggagagc tccacctcgc
     2641 cgccgagctc gatcaggtcg gataccagcc cgtccagctc ttgttggaaa gtctcgcgcg
     2701 gcacgaccta cccgaaccgt cccgtcacgt agtcctccgt ctccttgcgc tctgggttcg
     2761 agaacatctt ctccgtctcg tcgaactccc agaggcggcc aggatcaccc atgttctcga
     2821 taaagaaaaa gcccgtaaag tccgagatgc gggccgcctg ctgcatgttg tgcgtcacga
     2881 tcaccaccgt gtactcccgc ttgagttgct ccatggtgtc ttcgatcttc tgggtcgaga
     2941 cggggtcgag ggcgctggcc ggctcgtcca taaggatcac ctcaggctcg acggcgagcg
     3001 tcctggcgat gcaaagcctc tgctgctggc cgccggagag cccgtggccg ctctccttga
     3061 ggcggtcctt gacctcatcc cagagggcgg cgtgcttcag ggagcgctcc accaactcgt
     3121 ccatgttgcc cctgtagccg ttgatcctgg ccccccaggc cacgttctcg tagatggact
     3181 tggagaacgg gttgggcttc tggaagacca tccccacgcg gcgccggatg attacggggt
     3241 ccgaggcctt cgcgtagatg tcctgctcac ccagcctaac gtgtccctcg gcgcgggcgc
     3301 cgggtatcac ctcgtgcatc cggttcaagc agcgtataaa ggtgctcttg ccgcaccccg
     3361 agggcccgat gagcgcggtc accttgttgg cctgtatgtc catgctaacg tccgaaacgg
     3421 ccttaaagga accgtagaac gccgagaggt tctctatctt catacccagg gtgggagcct
     3481 cgcccgggga cacgccttcg ccgctctgtc gcgtctcgcg ctggtcctta gacaccccgg
     3541 acaacctctg ctcctctctg gatctcttag tgtccactag gctaacacct ttcttctcta
     3601 taagccttca ctacgaccgc tgctgatagc ggttgcgcag ccagatagcg aacgagttcg
     3661 ccaccaacaa caccaccatg gcaacgacta tgcctgccgc ggcagagttc tggaagacct
     3721 cctgcgggcg gctgatccaa tcgtatatct gggtggggag cgccatgtag ccgtcgaacg
     3781 ggcccgtggt gacgtaggtc tggtagagcg agaccccgac gaccaggatg ggagccgcct
     3841 cgccaacggc ccgcgagagc gccaggatga tgcccgtaat ggcaccgggc agcgcccgcg
     3901 gcaagaggtg actccagatc acctcccact gcgtcgcccc caacgcatag ccaccctccc
     3961 tgatagagtt cgggatcgcc ttgagcgcct ccctggtggc gacgatgatg accggcaaaa
     4021 tgagcaagct caaggtcatc gcgccggtaa gcacgctccc accgcccgtt atcggagcca
     4081 gaagctcgac gaagatgccg aggcccagga ggccgtagat caccgagggc accccggcga
     4141 ggtttgagat gttgacctcg atcatcctgt ttatgcgggt atccccggcg tactcctcga
     4201 ggtataccgc cgccccgagc ccgagaggaa cgctgatcgc gcccgtcaac ccgagcagcc
     4261 acaacgatcc gatgagggcc gggtagatgc ccccgttctc ggggaagacc tgcgagggga
     4321 agctcgtcag gaactcgagg ctgagcatcg aggacccgtc cctggtgacg tcgaccagga
     4381 gcacggccag aacggccacc ccgacgaacg ccgccaccga gacaagcacc gtgaagcccc
     4441 ggtcgaggcc gtaccgcgtg gacagagatt ccttgaagtc ccctccagag gggccgccgt
     4501 cctgctgccc ggctttcatg ctgctctggg ccactagtaa gtctccctgt atcgtctgac
     4561 gaaccagtaa ctcgcgaggt tcagggcaag ggtcatcaag aacagggccg atcccaccgc
     4621 gaatatggac tcgtaggtaa tcgaacctcg cggcgtctcc ccgcccacga cctgaacgat
     4681 gtatgccgtc atggcctgca taccttcgag ggggctcccg agccagttcg cctgggcccc
     4741 catcgcgatg gtcacgatca tcgtctcccc tatagcccga gagatgccga ggatcaccgc
     4801 cgccacgatg cccgatagcg ccgctggcac tacgatcctg gtcgccgtct ctcgtctggt
     4861 cgcgccgagc gcgtaggcgc cttcccgcag ggagagggga acggcgtaga tggcatcctc
     4921 gctcaccgat gccaccgtcg ggatgatcat cacccccatg atgagcccgg cggagagcgc
     4981 gttgaaggac tggatgcccg agaagacgct gtccctgaaa aacggggtga tgaaggtcag
     5041 ggcgaagaac ccgtagacga ccgtcggcac cccggccagt atctccagcg ccggcttgag
     5101 ccacctccgg agggcccgcg gggcgtactc gctaaggtag atggccgtga gcaaaccgag
     5161 gggaagcgcc acggcgatgg cgatgaaggt cgccgtaagc gtgccgtaaa cgagcggtaa
     5221 gacaccgaac tcgccctgcg ggggcgccca ctgcgtcgac gtcaaaaact ctacgggcga
     5281 gaccttgagg aagaaccgcg cggtctcttc gaagagcacg atgacgatcc cgaccgtggt
     5341 gaagatagag agataagcgc aggccatgag gaagaatttc acgacgcgct ccctggtctc
     5401 cctgccgcgg ctattgcgct tccacgcctc cgcgcctgac ctcgtacctt cagccctgat
     5461 cgccaactag aaccctccaa agtttcgttg acagaaagat ggggaccagc ctcggtgccg
     5521 gtcccccaat gctactagca gacctacccc cgcacaaatg gttactgtga ctgcttcagc
     5581 gccttctcaa ggtcgccgcc cttgggctgc ccgtcagcgt tgatcacggt gccagtagtt
     5641 cggtcctggt actgggcccg cgcctccttc tccaggctgg cgggcatcgt cacgtacttc
     5701 gcggcctcca ccagccggtc gaggttctcc ggcgcgaggt agaaatccac gaaaggcttg
     5761 acggccttgt tgttttcgag cgcgttggtg ctcacgtaga taaacagcgg gcgggagagc
     5821 aggtactcac cgttgcggat ggagtcctcg ctgggcttga cgccgtccac ggcgagcgcc
     5881 ttgagcgagt ccaggttgtt ctcgtagtag gagtacccga agtagccgag cgcgttggcg
     5941 tcaccagaga cgccctgcac gagcacgttg tcgtcctcgc tcatctccac gtcggactgg
     6001 cggctgacct cctcctcgtt ggcaacgata gcctcgttga agaactcgta cgtaccggac
     6061 tcggtgcctg gaccgtagag gtcgagtggc ttgttcggcc agtcagggtt gacctggctc
     6121 cactccgtga ccttgccctc ggcggaaggc tcccaaatct tcttgagctc ctcgctcgtg
     6181 atgtcggttg cccagtcgtt gtccttcttg ttcacgacca cggagatgcc gtcgtaggcc
     6241 accggtatct cgataaactc cacgttgttc tcttcgcaga cctcgatctc ctcgggctcg
     6301 atcggcctgg aagcgtccga gatctgggtg tcgccctggc agaaggcctc gaagccgtcg
     6361 ctggtccccg cgccacctac ctcgatgcgc gcgtccgggt tctcctccct gaagagttcc
     6421 gccgcggcct gggtgatcgg atagacggtg ctggagccct cgatacggat attgcccata
     6481 tcggcatcgc ccctcccacc ggcactcccg catgacacaa ggccaagcga caaccccagt
     6541 acgagacaca accacacaag cgacctctgg cgcgcgatcc ctctcaaccc gtacgctccc
     6601 ctcttccgga tgacatttct agatcgtaag actcatcgcg cacgatgata ggacaaagta
     6661 ctcggggttt agttgcagtt atgtaaactc gaagctaaat gtgtgttaaa cggcgcgctc
     6721 cggccgacag gccggagcgt gctcattccc actctatggt gcctgggggt ttgcttgtta
     6781 tgtccag
//
LOCUS       DBNGVD010000436         2674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_103067, whole genome shotgun sequence.
ACCESSION   DBNGVD010000436 DBNGVD010000000
VERSION     DBNGVD010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_103067"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="AC16CM_11745"
     CDS             <1..1890
                     /locus_tag="AC16CM_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR3140550.1"
                     /translation="VVLNRRPDSTERLLSLAETYRDRDGAGPDEKDLEWRSWPVERRI
                     EHALVKGIADFVEEDAEEARLEARRPLDVIEGPLMDGMNVVGDLFGSGRMFLPQVVKS
                     ARVMKKAVAYLVPFIEEAQDGVRRSNGTVVMATVKGDVHDIGKNIVGVVLQCNNFEVI
                     DLGVMVPSARILEVARERGADIIGLSGLITPSLDEMVHNAKEMQREGFEVPLLIGGAT
                     TSQTHTAVKIAPGYEHPVVHVKDASRAVGVVQNLISEDRKTSYADGIAADYERVREKH
                     AGRRSQTRLTTLAGARANGVRIDWERYTPPTPRVQGVRAFDDYPLEEIRPHIDWTPFF
                     HSWQLKGRYPRILKDSEKGEEARKLFADAQELLDRIVDEGWLRARAVFGLFPANAVGD
                     DSVELYTDGSRREVLADLNFLRQQKQKPPGRPNRCLSDFVAPKETGLEDHVGLFAVTV
                     GLGADAAARRFEEENDDYNAIMVKALADRLAEALAELLHQRVRTEFWGYAADEELDHE
                     ALIHEEYNGIRPAPGYPACPEHTEKRTLWRLLEVEDNAGIELTESCAMSPGAAVSGYY
                     FSHPDAQYFGVAEIGRDQARDYARRKDWTLEEAEKWLAPNLGYDPNTDPNGNGATEVP
                     QAVNK"
     gene            complement(1944..>2674)
                     /locus_tag="AC16CM_11750"
     CDS             complement(1944..>2674)
                     /locus_tag="AC16CM_11750"
                     /inference="COORDINATES: protein motif:HMM:NF021998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HIR3140551.1"
                     /translation="DRDTRERVSPETSRGHYLLEMFGIFCFAALSLLIAVELYGGVLR
                     FGDLWLAPILLVVAYLAADFLSGFVHFLADNFGSYDTPILGPNFIQPFREHHVDPKRI
                     VGHDFVDANGNNSLATLPFMLFVWIAVPLETWYYGYLFGTFFLFLCLAAFLTNQFHKW
                     AHMENPPAFVGWLQARGIILSQEHHDVHHESPYDTYYCITAGFWNPLLDRIRFYERTE
                     RVIRRFVPGTHPMLRSEREGNLNG"
BASE COUNT          540 a          803 c          912 g          419 t
ORIGIN      
        1 gtggtcctca accggcgccc ggactccacc gagcgcctcc tctccctggc ggagacgtat
       61 cgcgacaggg acggtgcggg gcctgacgag aaggatctgg aatggcgtag ctggccggtc
      121 gagaggcgga tagagcacgc gctagtcaag ggtatagccg atttcgtgga ggaggatgcg
      181 gaggaggcaa ggctagaggc caggcgtccc ctggatgtga tcgaaggtcc actcatggac
      241 gggatgaacg tggtcggcga cctgttcggt tcgggccgga tgttcttgcc gcaggtggta
      301 aagagcgccc gcgtgatgaa gaaggccgtg gcctacctgg tgccgttcat cgaagaggcc
      361 caggacgggg tgcgacggtc caacggcacg gtggttatgg cgaccgtgaa gggcgacgtg
      421 cacgacatcg gcaagaacat cgtcggggtc gtgctccagt gcaacaactt cgaggtcata
      481 gacctcggcg tcatggtgcc ttcggccagg atcctggagg tcgccagaga gaggggcgcc
      541 gacatcatcg ggctctccgg cctgataacg ccctccctgg acgagatggt ccacaacgcg
      601 aaggagatgc agcgcgaggg cttcgaggtt ccactcctga taggcggggc gaccacctcc
      661 cagacgcaca cggccgtcaa gatagcgccg ggctacgagc atcccgtggt ccacgtcaag
      721 gacgcctccc gcgccgtcgg cgtcgtgcag aacctcatca gcgaggaccg caaaacgtct
      781 tacgctgacg ggatagccgc cgactacgaa agggtccgtg agaagcacgc gggacgccgc
      841 tcacagaccc gcctcacgac gcttgcgggg gcgcgggcca acggcgtgcg gatcgactgg
      901 gaacgctaca cgccaccgac accaagggtg cagggcgtcc gggcgttcga cgactacccg
      961 ctggaggaga tcaggcccca catagactgg acgcccttct tccattcctg gcagctgaag
     1021 ggcaggtacc cccggatatt gaaggattcc gagaagggtg aggaggcccg caagctgttc
     1081 gctgacgccc aggaacttct ggaccgaata gtggacgagg gatggctcag ggccagggcc
     1141 gtcttcggcc tcttccccgc caatgccgtg ggcgacgact cggtggagct ctacacggac
     1201 gggtcgcgta gggaggtgct agccgacctc aacttcctgc gccagcagaa gcagaagccg
     1261 ccggggcgcc cgaaccgttg cctctccgac ttcgtcgccc cgaaggaaac gggcctcgag
     1321 gaccacgtgg ggctcttcgc cgttaccgtg gggctcgggg cagacgcggc ggcgcgccgc
     1381 ttcgaggagg agaacgacga ctacaacgcc atcatggtca aggcgctcgc cgacaggctc
     1441 gctgaggccc tcgcagagct gctacaccag cgggttcgca cggagttctg gggctacgcc
     1501 gcagatgagg agcttgacca cgaggccctg atccacgagg agtacaacgg tatccgaccg
     1561 gcccccggct acccggcctg ccccgagcat acggagaagc gcacgctgtg gcgactgctc
     1621 gaggtcgagg ataacgccgg catcgagcta accgagagtt gcgccatgtc acccggggcg
     1681 gcggtcagcg gctactactt ctcgcacccc gacgcccagt acttcggcgt agccgagatc
     1741 gggcgcgacc aggcaagaga ttacgcccgg cgcaaggact ggaccctgga agaggccgag
     1801 aagtggctag cccccaacct cggctacgac cccaacaccg acccgaatgg caacggggcc
     1861 acggaagttc cgcaagcagt taataaatag ctttcagaga tcagctttca gcttctgtgg
     1921 ctgaaggctg atcgcctgaa tcatcacccg ttcaggtttc cttctctttc ggaccttagc
     1981 attgggtggg tgccgggaac gaagcgccgg atcacgcgct ccgtccgctc gtagaagcgg
     2041 atgcggtcga ggagtgggtt ccagaacccg gctgtgatgc agtagtaggt gtcgtagggg
     2101 ctctcgtggt gtacgtcgtg gtgctcctgg gagaggatga tgcccctcgc ctgcagccac
     2161 cccacgaagg cggggggatt ctccatgtgt gcccacttgt ggaactggtt cgtcaggaac
     2221 gccgcgaggc acaggaacag gaagaaggtc ccgaacaggt agccgtagta ccaggtctcc
     2281 aggggcacgg cgatccacac gaagagcatg aagggcaggg tggccaggct attgttgccg
     2341 ttggcgtcga cgaagtcgtg gccgacgatc ctcttcgggt cgacgtggtg ctcgcggaag
     2401 ggttggataa agttgggccc caggatgggg gtgtcgtacg atccgaagtt gtcggctaga
     2461 aagtgcacga acccggagag gaagtcggcg gcaaggtagg ccacgaccag caggataggg
     2521 gccagccaca gatcgccgaa acggaggacc cccccgtaga gctcaacggc tatcagcaac
     2581 gagagagccg caaagcagaa tatgccgaac atctccagca agtaatggcc ccggctcgtc
     2641 tcggggctga cccgctcgcg cgtatcccgg tcct
//
LOCUS       DBNGVD010000437         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2655926, whole genome shotgun sequence.
ACCESSION   DBNGVD010000437 DBNGVD010000000
VERSION     DBNGVD010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2655926"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..250)
                     /locus_tag="AC16CM_11755"
     CDS             complement(35..250)
                     /locus_tag="AC16CM_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3140552.1"
                     /translation="MANKSWSNLSTGQRRGVVLSGVVQFVLLGAALVDIWRRPEEEIR
                     GSKRLWALASFVNFVGPVAYFAFGRKR"
     gene            complement(378..848)
                     /locus_tag="AC16CM_11760"
     CDS             complement(378..848)
                     /locus_tag="AC16CM_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140553.1"
                     /translation="MPDPQEVDPERSLPVWLRQASREQNGVAQVREPSPDTTTNLSGA
                     ALAFRTFHAAIAVEQLLAIAYVWWCALSGRRDRLLGIAAATLVCEGVLVGVNRGDCPL
                     GGLQERLGDPVPLFELVLSPHAAKRAVPTLGAVTAAGFALLMARGRRGVGRKER"
     gene            complement(886..1515)
                     /locus_tag="AC16CM_11765"
     CDS             complement(886..1515)
                     /locus_tag="AC16CM_11765"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3140554.1"
                     /translation="MGVGTMRRSPGQDYDLSDPVGSFVEVVRRVVTVPAGFFAGIPRR
                     GNYLAPLVFALVCIEISTILGGLLRLAVGAEAVGGVRFQGAGYGFGEFVADVILAPIG
                     GAIGLFLLAAIAHLLVMLFVGNGNSGFEATFRVVAYVSVTGLVNWIPLIGGLLALYGL
                     YLAVVGIREVHSTTTGRAALVVLLPVAVIFLLVALALAIAGAVILSQLA"
     gene            complement(1719..>2130)
                     /locus_tag="AC16CM_11770"
     CDS             complement(1719..>2130)
                     /locus_tag="AC16CM_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140555.1"
                     /translation="VPDKEYGKALRQLLRRIAETEADVLVAEVGASPLEPYNGEAAIA
                     QIGPNVRFTVLAASDPYAVAGVSAAFGSRPDLVTGLATSTTAGIELVEKLSKIPALNV
                     LDPGSLPKLRDMLMDRLDLHTGHLSHRSRLLGTT"
BASE COUNT          402 a          705 c          670 g          353 t
ORIGIN      
        1 gtctgcgggg tgccggcctg cacgatggac gactctagcg ctttcggccg aacgcgaagt
       61 aggcgaccgg tccgacgaag ttgacgaagg aggccagcgc ccacagccgc ttgctgcccc
      121 tgatctcctc ttcgggacgc ctccagatgt ctaccagggc ggcccccagc aacacaaact
      181 gcaccacgcc ggataacaca acgccccgcc gctgaccggt gctgagattg ctccaactct
      241 tgttagccac agccaagctc ctttcgcgaa gtgcctgttc actttgcatg gtcaaagtac
      301 cccaggcggg ccatgagggc ctgatccgca tgaaccatat cgctgatcaa taaagactac
      361 gtagccgttt cgaggggtta gcgctccttt ctaccgacac cacgccgacc gcgtgccatg
      421 agcagggcga acccggccgc ggttacggcc ccgagcgtcg gtacggcccg cttcgcggca
      481 tgcggggaga gcaccagctc gaacaacggc accgggtctc caagacgctc ctgcagacca
      541 cccagcgggc agtccccacg gttgacgccg acgagcacgc cttcgcacac gagcgtggcc
      601 gcggcgatgc caagcaggcg gtcgcgacgg cctgaaagtg cgcaccacca cacgtacgcg
      661 atggcgagaa gttgctccac ggcgatggcg gcgtggaagg tacggaaggc cagagcggct
      721 cctgaaaggt tcgttgttgt gtcggggctg ggttccctca cctgggcgac tccattctgt
      781 tccctgctgg cttgcctcag ccacacgggg aggcttcgct ctgggtccac ctcctgcgga
      841 tcaggcatcc tttccttcct gctgtgtgtc tcgtcaaccg gcagctcagg cgagctggct
      901 gaggatgacg gcgccggcga ttgcgagcgc caacgccacg agcaggaaga tcaccgctac
      961 cggcaacagc accacgagcg cggccctgcc cgtcgtggtc gagtgcacct cgcggatgcc
     1021 aacgacggcc agatacagac cgtatagggc gagcagtccg ccgatcaggg gtatccagtt
     1081 gaccaggccc gttacggaga cgtatgccac aacgcggaag gtcgcctcga agccggagtt
     1141 gccgttccca acgaagagca tcaccaggag gtgcgctatg gcggccagca agaagaggcc
     1201 gatggcgccg cctattggcg cgaggatcac gtccgcgacg aactccccaa agccgtatcc
     1261 tgctccctga aacctgaccc cgccaaccgc ctcggcaccc acggccagcc tcaggagacc
     1321 tcccaggatc gtggagatct cgatgcagac cagggcgaag acgagggggg ccaggtagtt
     1381 cccccgcctg ggtatccctg caaaaaatcc ggcgggaact gttacgaccc gccggacgac
     1441 ctctacgaag cttcccaccg ggtcggaaag atcgtaatcc tgcccagggg atctacgcat
     1501 cgtgcccacg cccatcttcg cgctcctctc gtctctgtgt tgcaagctaa caagacaacg
     1561 cccgcaaagg atgggccaca ttggcctgtt gactgggcaa tatttgtcaa gcttcctatc
     1621 ggggccggaa agggcagcgc tatacgccgg gtctcggacc atcgcgaaga acgcgcttca
     1681 ccgcggcgac ggctcggaac acggtcacgc cccggaggtc acgtggtccc gagcaggcgg
     1741 ctcctgtggg agaggtggcc ggtatggagg tccagcctgt ccatcagcat gtcgcgcagc
     1801 ttcgggagag aaccagggtc aagcacgttc agcgccggaa tctttgaaag tttctctacc
     1861 agttcgatgc cggccgtggt gctcgtcgcg aggccggtta cgaggtcggg gcggcttccg
     1921 aaggcggcgc taacgcctgc cactgcgtac gggtccgagg cggcgagcac ggtgaagcgc
     1981 acgttcggcc cgatctgcgc tatcgccgcc tcgccgttgt agggttcgag gggggaggct
     2041 cccacctcgg ccaccagcac gtcggcttcg gtttcggcta tgcgccggag gagctggcgc
     2101 aaggctttcc cgtactcctt gtccgggacc
//
LOCUS       DBNGVD010000438        11640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_649471, whole genome shotgun sequence.
ACCESSION   DBNGVD010000438 DBNGVD010000000
VERSION     DBNGVD010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_649471"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="AC16CM_11775"
     CDS             complement(<1..495)
                     /locus_tag="AC16CM_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140556.1"
                     /translation="MKHDNGPIRMRRIPGRFSRREFLGLGAAGAAGLMLAGCGTAGGA
                     SGGGGSSKLRIVYQPGTTTYAQLVIMEQQGWLARGLPDYDVSWTQVDAGAAVRDAIVA
                     GEADVGAGGIGPFLVGYDGGVDWRILSALNDIEMWLMVNEDRFQSLEDFGPRDKIAVV
                     APDSI"
     gene            complement(534..1388)
                     /locus_tag="AC16CM_11780"
     CDS             complement(534..1388)
                     /locus_tag="AC16CM_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140557.1"
                     /translation="MRSLSRDRMENVTFQGASGKRPEVGYWGRLPGFARYSVLVLALL
                     GAWQLYVSLSGIQPLILPGPVDVARAFVEGWVDGRLASATLVTLRTLCIGMVVGSVVA
                     LALTAFATWTQVGADLLSLLTSMLNPVPAIAILPLAILWFGLNPTALIFVIVYSITWP
                     IAINVSTGFRTVNPTLVMVGRNLDLGRWRMVRDVLFPAALPHTISGLRTGWAFGWRTI
                     VAAELVFGMAGKGGGLGFFINDARYFLRIPEVFAGIVTIALIGILVEALFSLLERRTV
                     IRWGMKSG"
     gene            complement(1372..2184)
                     /locus_tag="AC16CM_11785"
     CDS             complement(1372..2184)
                     /locus_tag="AC16CM_11785"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140558.1"
                     /translation="MLDTKEWRGVHGAYPLLSVEAVRLGYRVDGQTNVAVGNVSFQVW
                     PGEKVMLLGPSGCGKSTLLKAVAGFLEPDGGVISFAGQQDLRPRPDRAVVFQEFDQLF
                     PWRTVLDNVAYPLRATGRTEQEVVETAHRFVELTGLSHAVDRFPHQLSGGMKQRVAIA
                     RALALDPTMLLMDEPFGSLDAQTRARLQRELGEITRRTEVTVLFVTHSIQEALVLGDR
                     VVVLGHAPSEVRDVLEVTGAAGDPDSKRFAWTRRQLRRLLAEEEEVDDAVFE"
     gene            complement(2657..3517)
                     /locus_tag="AC16CM_11790"
     CDS             complement(2657..3517)
                     /locus_tag="AC16CM_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3140559.1"
                     /translation="MTLVLVLVAALLVGALIGCVGIGGVLLPPALVYLGGLGFHAAAA
                     TSTWAFLFCAAAGTWRYSGRGSIDWQMAGWLGAGVVPAAFLGARVNAALPDALLMSLL
                     AALMVLTGADVLLRRSRGSDGGARRLGAPALLAIGAFVGFGSALTGTGGAVLLVPILL
                     LLQIPVLTAVGAAQAVALPVAAFATAGFLLYGSVDFAFGTAAGLVGAAGVVAGAQIAH
                     AAPAAALRRVVAVALLCAGLLIAAQIAWGALRGDAARAADLASASATSVAREQLIPTG
                     SPVDFVPAWS"
     gene            3711..5075
                     /locus_tag="AC16CM_11795"
     CDS             3711..5075
                     /locus_tag="AC16CM_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140560.1"
                     /translation="MSAVERLGGEDTGRETSIRKVALASTIGATIEWYDFFLYGTAAG
                     LIFNQLFFPPGNPTASSLAAYATFAAGFVARPVGGFIFGHFGDRVGRKTMLVLTLLIM
                     GVATFAIGLVPTYAQIGIWAPVILLTLRVLQGIGLGGEWGGAVLMAVEHAPEGRRGFF
                     GSWPQLGVGAGLLLGTAAFSVLSIVLPEQAFLAWGWRAAFLASGILVAVGMYIRLQIM
                     ESPSFTEVKESGEEAAVPFLELLRTQPKESILSLGTRFAEGVSFNVYGVFVISYITVQ
                     LELPMTTALLGVSLAAAIMCVLTPVYGALSDRVGRRPVFVTGAVLFGLFALPSFLLFN
                     TRETVWIVLALVLAFGLIHPAMEGVLSSYWSELFHTRVRYTGVSFAYQFSGIFASGLT
                     PLVATWLLSAGGGRPWFLGAYMMGAALVTVISLFALQETYQRDIGPARAQAPEPEPEP
                     AMKG"
     gene            5079..5303
                     /locus_tag="AC16CM_11800"
     CDS             5079..5303
                     /locus_tag="AC16CM_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140561.1"
                     /translation="MRQKQRTGSALQRYFSRDSLYLPAIPKVQLALLAGLALMAFVLP
                     LTTSERLAVVVSAGVLAGLTWSTLNHVRRR"
     gene            5377..6501
                     /locus_tag="AC16CM_11805"
     CDS             5377..6501
                     /locus_tag="AC16CM_11805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR3140562.1"
                     /translation="MTQGETRVVPVSGKTIVKPLPTGSLVDYRKGEKEAGDPTGDLAL
                     NAEMRWEGMANAGYLTPIDRFFVRNHAPTPRVDPASWRLRVEGPGVGRTLELDYEDLT
                     RLSGISVVRALECAGNGRAFFGERQGREAPGTPWRLGAVGVAEWTGVPLREVLERAGL
                     GLTEGWVMAEGLDAVRMRRPLPLWKALGEDTILATGMNGQPLPPDHGFPARLVVPGWA
                     AVASVKWVGRIVVSDRPLFSPWNTAKYVMTGGRWGSRREPISVQVPKSAIELPWPAVL
                     RRGRNTITGRSWSGGAAIDRVEYAVDGDTRWRPARIEGPNLPGAWARWSFQWYARRGT
                     HELRVRATDTGGNTQPEAVAWNALGYLYGGIVGHPVEVLG"
     gene            6560..7093
                     /locus_tag="AC16CM_11810"
     CDS             6560..7093
                     /locus_tag="AC16CM_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3140563.1"
                     /translation="MHAIKVLREDHARIEALLEELADGGQVAELSERLKKELVVHILA
                     EDNVFLPQVADAIEDSRKATGEFFGGDPEVLAEASGLLTSSLENNAWVQELLQRLPSE
                     PTQKDIEDLQKAVERHVELEEGLFPRAQEVLEEEDFERIGDLIEHCKWQVRGLAQAKL
                     ASSSSFRPSSVEVTLLE"
     gene            7151..8377
                     /locus_tag="AC16CM_11815"
     CDS             7151..8377
                     /locus_tag="AC16CM_11815"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3140564.1"
                     /translation="MGRTAAEWGQKPPFPETHYVDSLVYTDEGIFEEEKHKIFAKTWM
                     LACHESELPEANDYRTYHHPAGVNLILVHGEDDVVRAFYNVCPHRGNTILYSPSGNAK
                     RMTCIFHAWSFDYEGNCVGLPREKAGYCSDGECSITRGDLGLREVRTQVGYGGFVYVN
                     LDDECDPLEEHIGSALDTIIEQIDTEPLEVFSYHRAVVDTNYKLWHDTNSEFYHDYLH
                     VHNRLTSMKQEGYFDRKMIPFPNGHAEVGSQEVHYEAYEGGKGRAEAGFPGVEGNPWS
                     LIDLFPGYSYNLRASVLRVDSQIPLGPNKTIVEYRSLGLKSDTEEQRNQRIQDNLVIW
                     GPFGRNLHEDLLAVDGQGRAIPNGSRYVLHGREEDSTIHDEVGMRHFYAEWSRRMGRL
                     SSDPTSKAAAGKPARI"
     gene            8454..8933
                     /locus_tag="AC16CM_11820"
     CDS             8454..8933
                     /locus_tag="AC16CM_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR3140565.1"
                     /translation="MDTATSKDLDTVVRDLIYRSCLRLDEGDFAGWIGLCAPEFGYRI
                     TAYSPEIRKEMIWLDHDREGMEGLFKMLPKHNSDHSPLTRHASVYTVEFDEASERASV
                     ITSLAVYRTQLDGGATSLFAVGKYFDTVTLEHGAPLLASRNVRLDTRELGIGSHLPL"
     gene            complement(8972..10114)
                     /locus_tag="AC16CM_11825"
     CDS             complement(8972..10114)
                     /locus_tag="AC16CM_11825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3140566.1"
                     /translation="MLFCLRLGEFDRILYGGGLVAVALATVLLIAVVVHPRSWLVSGL
                     LGSAPLRWVGVRSYGIYLWHWPVFMLTRPELDVPFDGLPLLALRLGLTVVLADLSYRL
                     VERPIRRGALGRAWKALREARGARRRRLRLQWAGAVLPIAALCMALGVATALAEPPKP
                     PSYASTKHVRSESGDQARAQKTGAPGKDASGKAGPSESTGGRHAAEARGGARQDLRSE
                     PGPRGRVTAIGDSVMLGAVDNLQQDIPRLTTIDARGSRQIPEATRILEQLDASGDLGS
                     VVILHIGDNGAITDEQFDQAMDVLSVTKTVLVVNTTVPEGYQWAPNNEVLADGVARYP
                     ARAVLVDWYARSKGHPEYFNDGLHLTPSGARAYADLIASTYRAQER"
     gene            complement(10024..10902)
                     /locus_tag="AC16CM_11830"
     CDS             complement(10024..10902)
                     /locus_tag="AC16CM_11830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3140567.1"
                     /translation="MRLTYSPGLDGLRALAVAAVLLYHADLSFLPGGFLGVEVFFVVS
                     GYLITALLLVEWRREGHISLKSFWLRRARRLLPALYVLLVATLAYSVVFLPGEVAGLR
                     DDAIAAVGYVTNWFLIVGQESYFEAVGRPSLLQHLWSLAVEEQFYLIWPIVLVLGLGV
                     GTTYRRQRVALAVALAGAVASAVAMALLYTPGVDPSRVYYGTDTRATGLLCGAVLAFL
                     WSPAEKYRPPDKWAARRNRGRRRSRLRPATPLVVDAVGVFSLQPLCSFACALGSLIAS
                     STGAGWWRWPSPRCSS"
     gene            complement(11213..11482)
                     /locus_tag="AC16CM_11835"
     CDS             complement(11213..11482)
                     /locus_tag="AC16CM_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140568.1"
                     /translation="MNGLLGKGRSGGWRLGEVSGSAVLLVLLFSEVASLVTGMGALYF
                     ASIVAAWLVLPAVVKFENVLDAGCFFMLVSLGILVVVDLAGQGQV"
BASE COUNT         2179 a         3820 c         3736 g         1905 t
ORIGIN      
        1 aatggagtcg ggggcaacga ccgctatctt gtccctgggt ccgaagtcct ccaggctctg
       61 gaacctgtcc tcgttgacca tgagccacat ctcgatgtcg ttgagggcgc tcagaatccg
      121 ccagtccacc ccgccgtcgt agccgacgag gaagggccct atccccccgg cccccacgtc
      181 cgcctcgccg gcgactatgg cgtcgcgcac cgcggcccca gcgtcaacct gggtccaact
      241 gacgtcgtag tcgggcaggc ctctggcgag ccacccctgc tgctccatga tcacgagctg
      301 cgcgtaggtc gtggtcccag gctggtatac gatcctgagc ttgctgctac ctcccccgcc
      361 cgacgcgcca ccggccgtcc cgcaccctgc cagcatcagc ccggcggcac ccgcggcccc
      421 gagcccgagg aactcccgcc ggctgaagcg tcccggtata cggcgcatcc ttatgggccc
      481 gttgtcgtgc ttcatgcttc tcctttctct ccctgcaggt ttcaccggat cagctatccg
      541 ctcttcatgc cccaccggat caccgtccgc ctctcgagga ggctgaagag cgcctcgacg
      601 aggatgccta tcagggcgat ggtcacgatg ccagcgaaga cctcggggat gcgcaggaaa
      661 tagcgggcat cgtttatgaa gaagccgagc ccgccgccct tgccggccat cccgaagacc
      721 agctccgccg cgacgatggt gcgccagccg aaggcccagc ccgtgcgcag gcctgagatg
      781 gtgtgcggca gggcggcggg gaagagtacg tcgcggacca tgcgccaccg gccgaggtcc
      841 aggttgcggc cgaccatgac aagcgtcggg ttgaccgtcc ggaaacccgt gctcacgttt
      901 atggctatgg gccaggttat ggagtagacg atcacgaaga tgagcgcggt cgggttgagg
      961 ccgaaccaca ggatggctag cggtaggatg gctatggccg gcacggggtt gagcatggag
     1021 gtcaagagcg agagaagatc ggcccccacc tgcgtccagg tggcgaacgc ggtgagggcg
     1081 agggccacga ccgagccaac caccatcccg atgcataaag tccttagcgt caccagcgtg
     1141 gccgaggcga gccgcccgtc gacccacccc tctacgaagg ctctggccac gtccacggga
     1201 ccgggtagta tgaggggctg tatgcccgag aggctaacgt agagttgcca cgccccgagg
     1261 agcgcgagca cgagaacgga gtaccgcgcg aagccgggca gccttcccca gtagccgacc
     1321 tcgggtctct ttccggacgc cccctggaag gttacgtttt ccatcctgtc cctactcaaa
     1381 gaccgcatcg tccacctcct cttcctcggc gagcagcctg cgcagctgcc ttcgggtcca
     1441 ggcgaaccgc ttcgagtccg ggtcgccagc cgccccggta acctcgagca cgtccctgac
     1501 ctccgatggg gcgtgcccga gcacgacgac ccggtcgcct agcaccagcg cctcctggat
     1561 gctgtgggtg acgaaaagaa cggtcacctc ggtccgccgg gttatctcgc ctagctctct
     1621 ctgcaacctc gcgcgggtct gggcgtcgag gctgccgaag ggctcgtcca tgaggagcat
     1681 cgtgggatct agcgcgaggg cgcgcgctat cgcgacgcgc tgcttcatgc cgcccgagag
     1741 ctggtgcggg aaacggtcga ccgcgtgcga gaggcccgtc agctccacga accgatgggc
     1801 cgtctccaca acctcctgct ccgtccggcc cgtcgcccgc agcgggtagg ccacgttgtc
     1861 cagcaccgtg cgccagggga agagctggtc gaactcctgg aataccaccg ccctgtcggg
     1921 cctgggccgc aggtcctgct ggccggcgaa cgagatgacg ccaccgtccg gttccaggaa
     1981 ccccgccacg gccttcagga gcgtggactt gccgcacccg ctcgggccga gcagcatcac
     2041 cttctccccc ggccacacct ggaagctcac gtttccaacc gccacgttgg tctggccgtc
     2101 taccctgtat ccgagcctga cggcctccac ggagagaagc ggatacgcgc cgtgaactcc
     2161 tcgccattct ttggtatcga gcatgtcgat ctcctcgcgt gttcttctac gtgttctcgc
     2221 cggccttccg aggctggatc tggggaagtg ccggcggcgg ggtaggaccg ccggctcggg
     2281 aagaaagcta ctctagagtg gatgcccacc tcggtgcctt tcaccttcac gcgggaggtg
     2341 cggcggtgcc cccactaccc tactgtcgcg tacccccgca cgcgtcctcc tcgtcacgtc
     2401 agtcatgctg ccaacctcct ttatacagtt tgttggtcga ttaacttaac aaaatgagag
     2461 gctaccccga gaccgagcgg gggtcaagtg attctggtgg cgaaaaaggt gggtctggcg
     2521 cgtcatggtc gaccgcagaa agtccagagt cgccattgca ccgccccaaa cccgcaacaa
     2581 aagcaattca aagaaatccg aaaaaatgtt caaaaaagtt cacaaaacgc cgtagtctgt
     2641 ggttccgggg gggtggctag ctccaggcag ggacgaaatc gacgggagag ccggtgggga
     2701 tgagttgctc tctcgcgacc gaggtcgcgg aagccgacgc aaggtcagca gccctggcgg
     2761 catcgccgcg aagcgccccc cacgctatct gtgccgcgat gaggagtccc gcgcacagga
     2821 gggctacggc caccacgcgc cgcagcgcgg cggccggtgc ggcgtgggcg atctgggctc
     2881 ccgcgacgac acctgcggcc cccacgagcc ccgccgccgt accgaacgcg aagtcgaccg
     2941 aaccgtagag gaggaagccg gcggtggcga aagccgcaac cggcagggcg acggcctggg
     3001 cggcgcctac cgctgtaaga accggtattt gcaggagcag gaggatcggt acgagcagta
     3061 ccgccccgcc ggtgcccgtg agcgcggagc cgaagccgac gaacgcgcca atggcgagca
     3121 gggcgggagc cccgaggcgg cgcgcgcctc catcgctgcc acgggaccgt ctgagcaaga
     3181 catcggcacc cgtgagcacc atcagtgcgg cgaggaggga catcagcagg gcatccggca
     3241 gggcggcgtt gacccgggct cccaagaacg ccgccgggac aacgcccgcc cccagccacc
     3301 cagccatctg ccagtcgatg ctgccgcgcc cggagtatct ccacgtaccc gccgccgcgc
     3361 agaacaagaa agcccacgta ctcgtcgccg cggccgcgtg gaagccgagg cctcccagat
     3421 agaccagggc gggcggcaac aggacgcctc cgataccgac gcagccgatc agtgcaccca
     3481 caagtaaggc ggcaaccagc acaagaacta gcgtcacccg ttcgttctta cccttactcc
     3541 cgacacttct aatctcacct cctcccaacc gctagtcgat cgcgaaactc ctgaagaatt
     3601 tgccgtcgtg gccttgactc cgtagatgcc ccggggtatc ttcttcgctt catcataaaa
     3661 ataatagaga aagttaaaaa tagttccaaa ggcttcgagg gaggcgacag atgagcgcag
     3721 tggagaggct tggcggggag gatacggggc gggagacctc gatcaggaag gtcgcgctgg
     3781 ccagcactat cggcgccacc atcgagtggt acgacttctt tctctacggc acggcggcgg
     3841 ggctcatctt caaccagctc ttcttccctc ccggcaaccc cactgctagc tcgcttgcgg
     3901 cctacgccac ctttgcggcc gggttcgtag cccggccggt aggtggtttc atcttcgggc
     3961 acttcggaga cagggtgggg cgcaagacca tgctggtatt gacgctgctc atcatggggg
     4021 tggccacgtt tgccatcggg ctggtgccca cctacgcgca gatcgggatc tgggcccccg
     4081 tcatactcct tacgttgcgg gttctgcagg gcattggcct cggtggggaa tggggcgggg
     4141 cggtcttgat ggccgtcgag cacgccccgg aaggcaggag gggtttcttc gggagctggc
     4201 cccagctagg tgtgggcgcg gggctgttgc tcggcaccgc ggccttcagc gtgctttcga
     4261 tcgtgttgcc cgaacaggcc ttcctcgcct ggggttggag ggcggcgttc ctagctagcg
     4321 gcatcctggt cgcggtgggc atgtacatcc ggctgcagat catggagagc ccgtccttta
     4381 ccgaggtcaa ggagtcagga gaggaggccg cggtgccgtt cctggagcta ctgcgcaccc
     4441 agccgaagga gtcgatcctc agcctgggca cgcgtttcgc cgagggtgtc tccttcaacg
     4501 tctacggggt cttcgtgatc tcctacatca ccgtgcagct cgaactgccc atgaccacgg
     4561 ccctcctggg cgtaagcctg gcggcggcca ttatgtgcgt cctgaccccc gtctacggtg
     4621 cgctctccga cagggtgggc agaaggcccg tgttcgttac cggcgccgtt ctgttcgggc
     4681 tctttgcgct gccctccttc cttctgttca acacgcggga gaccgtgtgg atcgtgctgg
     4741 cgctcgtgct ggcgttcggg ctcatccatc ctgccatgga aggcgtactg tcgtcgtact
     4801 ggtccgagct cttccacacc agggtgcgct acacaggggt ctccttcgcg taccagttct
     4861 cgggcatctt cgccagcggt ctcaccccgc tcgtcgccac ctggctgctc tctgcgggag
     4921 ggggccgacc ctggtttctc ggggcgtaca tgatgggtgc ggcgctcgtg accgtgatct
     4981 cgctcttcgc cctccaggag acctaccaga gagacatcgg acccgcgagg gcccaggcac
     5041 cggaaccaga acccgaaccg gccatgaaag gttagggcgt gaggcagaaa cagcgcaccg
     5101 gtagtgcgct ccaacgctac ttctccaggg atagcctcta tcttcccgcc atcccaaagg
     5161 tccaactggc gttactggcc gggttggcgc tgatggcttt cgtactgccg ctgaccacgt
     5221 ctgagaggtt ggcggtcgtg gtctcggctg gtgtgctggc ggggttgacg tggtccacgt
     5281 tgaatcacgt gaggcggaga tgacctcggt cagcgcgacg ggttgtaagc cccgttcgtc
     5341 gtcccactct gccacctgca ataacgggag ggaagcgtga cgcagggcga gacgagggtg
     5401 gttccggtct ccgggaagac catcgtcaag ccgctaccaa ccgggtccct cgtcgattac
     5461 cggaagggtg aaaaagaggc cggggacccg acgggcgacc tcgccctgaa cgcggagatg
     5521 cgctgggagg ggatggcgaa tgccgggtac cttaccccca tcgaccgctt cttcgtgcgc
     5581 aaccacgcgc cgaccccgcg cgtggacccc gcaagctggc ggctccgcgt tgagggcccc
     5641 ggcgtcgggc gtacgctgga attggactac gaagacctca cccggctctc tggcatctcg
     5701 gtggtacggg cacttgagtg cgcgggcaac gggcgcgcct tcttcggtga gagacagggc
     5761 agggaggccc ctggcacgcc ctggaggctg ggggctgtcg gggtcgccga atggacgggg
     5821 gtgcccctgc gcgaggtcct ggaacgggcc ggcctcgggc tgaccgaagg ttgggtgatg
     5881 gcggagggcc tcgacgcggt cagaatgcgc cgtccgctgc cgctctggaa ggccctcggg
     5941 gaggacacga tcctcgccac cggcatgaac ggccaacctc tgccgccgga ccatggcttt
     6001 ccggcccggc tcgtggtgcc tggctgggcc gcggtggcga gcgtgaagtg ggtcggtcgc
     6061 atcgtggttt cggaccgccc gctgttctcg ccctggaaca cggccaagta cgtcatgacc
     6121 ggaggcaggt ggggctcccg gcgcgagccc atcagcgtgc aggtgccgaa gagcgccatc
     6181 gagttgccgt ggccggccgt gctgcggcgt ggaaggaaca ccataacggg acgctcctgg
     6241 tccgggggcg ccgcgatcga tcgggtggag tacgcggtgg acggcgacac gcggtggcga
     6301 cccgcgcgga tcgagggccc aaaccttccc ggggcgtggg cgcgctggag cttccaatgg
     6361 tacgccagac ggggaacgca cgagttgagg gtcagggcca cggataccgg gggcaacacc
     6421 cagccggagg cggtggcctg gaacgcgcta gggtacctct acggcggcat cgtggggcac
     6481 ccggtcgagg tgctcggata gtggcaccag caggagacga ggtccgacct tcgatgcggg
     6541 ccgtcaggag gttgatggga tgcatgcgat aaaggtgctt agagaagacc acgcgaggat
     6601 cgaggcgctc ctcgaggagc tagccgacgg gggccaggta gcggagctct cagaacgttt
     6661 gaagaaagag cttgtggtcc atatattggc cgaagacaac gtcttcttgc cgcaggtcgc
     6721 ggatgccatc gaggacagca ggaaagcgac tggagagttc ttcggaggag accccgaggt
     6781 cctggcggag gcttcggggc tactgacaag cagcctcgag aacaacgcgt gggttcagga
     6841 actgttgcaa cgactaccat cagaacctac ccagaaggat atagaggatt tgcaaaaggc
     6901 cgtggaaagg cacgtcgagc tggaagaggg cctgtttccg cgggcgcaag aagtactgga
     6961 agaagaagat ttcgagagga tcggggacct catcgaacac tgcaaatggc aggtgagggg
     7021 cctggcgcag gcgaagctgg cctcatcgtc cagcttcagg ccatcatccg tggaggtcac
     7081 gctactcgaa taggcaaagg cagtggaggg cttccagcct gttggcacaa cctacgagga
     7141 ggagagaaag atgggacgca cggcggccga gtggggacag aaaccaccgt ttccggagac
     7201 gcactacgtg gactcgctcg tctacacgga cgagggcatc ttcgaagagg agaaacacaa
     7261 gatcttcgcg aagacctgga tgctcgcctg ccacgagagc gagttaccag aggccaacga
     7321 ctacaggacc taccaccatc cggccggggt gaacctgatc ctggttcacg gagaggacga
     7381 tgtggtcagg gccttctaca atgtctgccc acacaggggc aataccatcc tctacagccc
     7441 ctctggcaac gccaagcgca tgacgtgcat cttccacgcc tggtccttcg actacgaggg
     7501 gaactgcgtc ggcctccctc gcgagaaggc cggttactgt tcggacggtg agtgcagcat
     7561 aaccagagga gacctcggcc tgcgtgaggt cagaacccag gtcggctacg gtggcttcgt
     7621 ctacgtgaac ctcgacgacg agtgcgaccc gctagaggag cacatcggca gcgcactcga
     7681 caccatcatc gagcagatag acacagagcc cctggaggtc ttctcgtatc acagggcggt
     7741 tgtagacacc aactacaagc tctggcacga cacgaacagc gagttctatc acgactacct
     7801 ccacgtccat aacaggttga ccagcatgaa acaggagggg tacttcgatc ggaagatgat
     7861 ccccttcccc aacggccacg ccgaggttgg gtcccaggag gtccactacg aggcttacga
     7921 aggcggcaag ggcagggcgg aagccggatt cccaggggtg gagggaaacc cctggtccct
     7981 gatcgacctg tttcccggct actcgtacaa cctgagggca tcggtgctgc gcgtagactc
     8041 gcagataccc ctcggtccaa acaagaccat cgtcgagtac cgcagcctcg ggttgaagtc
     8101 cgacaccgag gagcagcgaa accagcggat ccaggacaac ctggtgatct ggggtccttt
     8161 cggccgcaac ctgcacgagg acctgctcgc cgtcgacgga cagggtcgcg cgatcccgaa
     8221 cggctcgcgc tacgtgctcc acggccgcga ggaggacagc accatccacg atgaggtcgg
     8281 catgcgtcac ttctacgcgg agtggagcag gcgcatggga cgcctctcca gcgaccccac
     8341 aagcaaggcg gcggccggga aaccagccag gatctgaccc cgcgcgaacc gctggccagc
     8401 cctggctccg cgcaaggacg gcaacaaaga agaaggagga cacagacgtg accatggata
     8461 cggcgacgag caaggacctc gacacggtgg tgcgggatct gatctaccgt tcctgcttgc
     8521 gcctggacga gggcgacttt gctggatgga tcggactctg tgcccccgag ttcggctaca
     8581 ggatcacggc ctacagcccg gagattcgca aggagatgat ctggctcgac cacgaccgcg
     8641 agggtatgga gggcctcttc aagatgctcc ccaaacacaa cagcgaccac tcccccctca
     8701 cccgccacgc cagcgtctat accgtcgagt tcgatgaggc cagcgagagg gcgtcggtta
     8761 tcacctcgct ggccgtctac agaacgcaac tggacggtgg cgccacaagc ctcttcgccg
     8821 tcggcaagta cttcgatacc gtcaccctgg agcacggagc tcccctcctg gcaagccgca
     8881 acgtcaggct ggatacgcgc gaactgggta tagggtcgca cctgcccttg tagagccgcc
     8941 agctctcagc ttctggctct gcttttcctg gctacctttc ctgcgcgcgg taggtggagg
     9001 cgatcaggtc ggcgtaggcc ctcgccccgc tgggcgtcag gtgcagcccg tcgttgaaat
     9061 actcggggtg ccccttgctc ctggcgtacc agtcgacgag cacggccctg gccgggtagc
     9121 gggcgacgcc gtccgccaga acctcgttgt tcggtgccca ctggtaaccc tcgggcacgg
     9181 tggtgttcac caccaggacc gttttcgtaa ccgagaggac gtccatcgcc tggtcgaact
     9241 gttcgtcggt gatggctccg ttgtcgccta tgtggaggat caccacgctc ccgaggtcac
     9301 cggaggcgtc cagttgctcg aggatgcgtg tggcctcggg tatctggcgg ctgccgcggg
     9361 cgtcgatggt ggtcagcctg ggtatatcct gctgcaggtt atcgaccgcg cctagcatca
     9421 cggagtcgcc gatggccgtc actcgtcccc gggggcctgg ctcgctgcgg aggtcctgcc
     9481 tggccccgcc ccgcgcttcc gccgcatgac gcccgcccgt gctttctgaa ggcccggcct
     9541 tgccggacgc gtctttcccc ggtgcgccgg tcttctgtgc cctggcctgg tctccgctct
     9601 cgctcctgac gtgcttggtg gaggcgtagg agggcggttt cggtggttcc gcgagcgccg
     9661 tcgcgacgcc gagggccatg cacagggccg ctatgggcag gacggcgccg gcccactgca
     9721 ggcggagccg cctgcgcctc gctccccgcg cctccctcag cgccttccag gcccgaccca
     9781 gagccccacg ccttatgggc ctctccacca ggcggtaaga gaggtccgcc agcacaaccg
     9841 ttaacccaag ccgcagcgcc agcagcggaa gcccgtcaaa gggtacgtcc agctcggggc
     9901 gtgtcagcat gaataccggc cagtgccaca gatagatccc gtatgagcgt acccccaccc
     9961 accggagagg agccgaacca agaagccccg agaccagcca cgacctcggg tgtaccacga
    10021 ccgctatgag gagcaccgtg gcgagggcca ccgccaccag cccgcccccg tagaggatgc
    10081 gatcaaactc cccaaggcgc aggcaaaaga gcacaagggc tgcagggaga agacgcccac
    10141 cgcatcaacg accagcggcg tagcaggccg caaccggctc cgcctgcggc cccggttgcg
    10201 tcgggcggcc cacttgtctg ggggtctgta cttctctgcg ggcgaccaga ggaaggcaag
    10261 cacggcccca caaagcagcc ccgtggcgcg cgtgtccgtg ccgtagtaga ccctcgaagg
    10321 gtccaccccg ggcgtgtaca gcagggccat cgccaccgcc gaggccacag cgcccgccag
    10381 cgccacggca agtgctaccc gctgccgtcg atacgtggtc ccgaccccaa gccccaggac
    10441 gagtactatg ggccagatca ggtagaactg ctcctctacc gccagggacc acagatgctg
    10501 caacaaagag ggcctgccaa ccgcctcgaa gtaagactcc tgccccacta taaggaacca
    10561 gttcgtcacg tatccgacgg ctgctatggc atcgtccctg agccccgcca cctcccctgg
    10621 caggaaaacg accgagtagg cgagcgtcgc caccagcaac acgtagaggg cgggcagcag
    10681 cctcctggcg cgcctgagcc agaagctctt caagcttatg tgcccctctc ggcgccactc
    10741 gacgagcagc agggcggtta tgaggtagcc gcttacgacg aagaagacct ccacgcccag
    10801 gaagccaccc ggcaggaagg ataggtcggc gtggtagagg aggacggccg caacggcgag
    10861 tgccctgagg ccgtcgaggc cggggctgta agtcaggcgc acctcggggg tgctcggcac
    10921 agaaccagac cgtgttgcac ggtcgaacgc cgactcctca ccggctgcgc gcgacctttg
    10981 tagcatacgt gttctcgtgc tcctatcccg cggtttgcgc atgggatgct agcatagtta
    11041 tcaaccctga gattccttac acaacggctg ggtgccggga agcgatgaca cccattacct
    11101 ggggcttcgg ggtaccagag gccgatgtcg cggagattcc cccgcgaaga cgggacatct
    11161 cttggtttcc tcagggctcg acaacgagat ccgacgccac ctataagagg agtcagacct
    11221 ggccctgccc ggcaagatct accactacca ggatcccgag cgagacgagc atgaagaagc
    11281 atcccgcatc gaggacgttc tcgaacttca cgacggctgg tagaacgagc caggccgcca
    11341 cgatggacgc gaagtagagc gcgcccatcc ccgttaccag ggatgccacc tccgagaaga
    11401 gcaacaccaa cagcaccgcc gaaccggaga cctcaccaag ccgccagcca ccgctacgcc
    11461 cctttcccag aagaccgttc aagcaccctc cgaaggacgc tcggagatct catccaggcc
    11521 ctccgcatcc gacgccactg caggcccctc atctccctcg cccttgacgt cgtaccaggt
    11581 atccaacccc tggcttccgg ccctgtccca caactcgtac cagagatcca actcccactt
//
LOCUS       DBNGVD010000439         7695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2472507, whole genome shotgun sequence.
ACCESSION   DBNGVD010000439 DBNGVD010000000
VERSION     DBNGVD010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2472507"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC16CM_11840"
     CDS             <1..465
                     /locus_tag="AC16CM_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3140569.1"
                     /translation="GSGTSSWSSRLIHGGLKYLEKLDVGLVRESLREREWLLSSAPHL
                     VRPLRMILPFYSGGKHGPLSLEAGMVAYDVLSWDKSVPHHSVWGKRGVLRRAPGLETE
                     GLSGAAASSVRVTERLEPDADAHERLDEKYGHYRRVIDALEEAWEVSPGEPA"
     gene            539..1543
                     /locus_tag="AC16CM_11845"
     CDS             539..1543
                     /locus_tag="AC16CM_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3140570.1"
                     /translation="MEVTEVDRPEVGPEEILIKVGANTVCGTDVRIVTGQKTSGVRRP
                     SIIGHEFAGKIAQVGERVRGYEQGMPVAVAPTIPCHRCYYCQHGMENVCDDKRRLGYE
                     LDGGLGEYVLVPADGVSSGNVFVVGDDTPVEHLALAEPLACCVNGQRQTPVGLNDTVL
                     IMGAGPIGLFHLQLALLSGAETVIVSQPSAPRREFAAGLGAHLTVNPGEENLSSVVAE
                     ATGGLGVDVAIVCIGKPGLVNDALGLVRRGGSVNIFAGLSGEGWAELEANRIHYDQLR
                     VTGVSDCSRSDYEAALRLIRSGRVDVSKMVTHRFPLAEVNAALDSTANGEGIKAAVVP
                     "
     gene            1644..2906
                     /locus_tag="AC16CM_11850"
     CDS             1644..2906
                     /locus_tag="AC16CM_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3140571.1"
                     /translation="MGLREKLEVLEREGTPIRIGLVGAGQMGRGLIAQIAGIPGMEVT
                     AVADIAPERAMSAFREIGQEPVTDREGRPGRPNVTEDAGDLSHSDTVDVVVEATGVPE
                     IGARVAFDAIHNGKHVVMLNVEADVTIGPILTRMGRSAGVVYTGSAGDEPGAIMELYD
                     FARSLGFEVVAAGKGKNNPLDFTATPDSLADEAATKHMNPKMLASFVDGTKTMVEMAA
                     VANATGMTPDTPGMHGPEETVPNRLADLFSLEKEGGILSSYDSVDYVRGIAPGVFVIV
                     QAAAEPVRETLEYLGQGAGPNHVLYRPYHLTSLETPISAARAVLYGEPTIASRDNPSA
                     EVVAVAKRDLEPGETLGSIGSADYYTSIYGVDEARDMLPMGLAEGGRTKRAIAQGDVI
                     CRADVEQASGTFVGDLRRLQETALGWKS"
     gene            complement(2975..3733)
                     /locus_tag="AC16CM_11855"
     CDS             complement(2975..3733)
                     /locus_tag="AC16CM_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3140572.1"
                     /translation="MAETPDRYCSNCGHELDTEDQFCPNCGSPVHEAATVPTPEADVP
                     VPPPPPQAAAAAAPPPQQAEDAQRPRSRRNLFIAGCLGLIGVLALLIIAIVAIVALSG
                     GGDEPATKDNPPAKAPANTPESTPTSTPESTPTEAPADDTGTITMSDVKMAADENGDQ
                     PTTVFSPKDTFYCVGYLEGAPDDTKITAVWLAADIENIEPNSKIKQISATGGDGLFRF
                     NLSPGESWPTGEYAVQLYLNDAEQPTESLTFEVR"
     gene            complement(3952..4161)
                     /locus_tag="AC16CM_11860"
     CDS             complement(3952..4161)
                     /locus_tag="AC16CM_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140573.1"
                     /translation="MLDRRSFEEAKSRLPELTGYMEIEGMNHAQFGSYGPQEGDGKPT
                     VTDGRAVREVVDASKDLVQGGAGIR"
     gene            complement(4092..4685)
                     /locus_tag="AC16CM_11865"
     CDS             complement(4092..4685)
                     /locus_tag="AC16CM_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140574.1"
                     /translation="MNLARILLVAASGVVAVVVCALTWWTFGSYKPDAVAVAAMSGAT
                     DEGGWYEFGGRESCTGVVLYPGARVDAEAYAPLASELSEQTGAMVAVVRVPLDMALLD
                     GDAADEVIAAHPEVRRWTLAGHSLGGVAAAAYAEERAGETVDGLLLFASYPSGARISR
                     MTRSRSRAYLGPGIECWTVGLSKRRRAVFPNSPDTWR"
     gene            4941..5744
                     /locus_tag="AC16CM_11870"
     CDS             4941..5744
                     /locus_tag="AC16CM_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140575.1"
                     /translation="MGKAAEAPTTATLDWWRIGVALFGAFFQVIVGFFVPVGSLVNRE
                     VSLIIPAGWTFAIWGPIFLLCFLFAGYQLLPSRRYDPLLRSVGWPLGLAFVGNGLWTL
                     IQPFRQPILSQVVIFLILILALTALVRFARFGAVSPVQRWIVGLPVGALAGWLTAANV
                     VGFNDMLVREDVVGSSVLAALVGAGLLVVGTIVAARVLAVSFAGPLQASIAYAAAVLW
                     GLFGIAANQYDSSVITTLTSVVCFVTLAYLSVLTLVRRRGHGAPRGRAV"
     gene            5898..>7695
                     /locus_tag="AC16CM_11875"
     CDS             5898..>7695
                     /locus_tag="AC16CM_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3140576.1"
                     /translation="MFAAWGRFVYRFRWLVLFLSILSIAAVAYSAQYGGRLGTGEFAA
                     PEGTESGRVDVLLDEEVAKTPPAFTLIFGSDEEKATDPAFRDGVERALGPLEDDDRVT
                     NVRTAYENGAVDGDMISTDEHHSLVVVELEEGSFTERQDAYVDLRQEVREGPFEVVAA
                     GDMPLNEDFERVTERDLKRAEVVSLPLALLMLLFVFGSVVAAGLPIGVGILAVAGGFT
                     GTLLLARLMDVSIYATNIVTMIGLGVAIDYSLFVVSRFREEVGRHPVPEALARTMATA
                     GRAILFSGLTVAIGFMGFLFFDVADLNSIGFAGSIVVAFSVLYGLTFLPALLAILGPR
                     VDFLRLPFVRPGRVATGTGPWHRLATAVMAHPWRTLLPVVALLLVLGSPFLNIRLGLS
                     GADTLSRDSESRLGEELLDRQFPDTSAYPMVVVLDYPQGSSLSKERVGETFDLSRWLE
                     HRKGVKDVESVVNLGPGLTRDQYQELLSGPRGDLPPEIRNALKRTTGDHLTVLTAYTS
                     VGYNSDEAYDLVREVRERHPDVGGEVLVGGWAAFDLDLTESVYEAAPPAIAFVVLATY
                     VVLFLLLGSVLLPIKAVVVNFLSISASYGALVW"
BASE COUNT         1292 a         2434 c         2583 g         1386 t
ORIGIN      
        1 gggagcggaa catcctcctg gtcgagccgg ctcatacacg gcggcctgaa gtacctggag
       61 aagctcgacg tcgggctcgt cagggagtct ctgcgagaac gggagtggtt gctctcatcg
      121 gcaccccacc tggtccgacc tctaaggatg atcctgccat tctactcggg ggggaagcac
      181 ggtcctctct cgctagaggc ggggatggtt gcctacgacg tgctctcctg ggacaagtcc
      241 gtaccccacc actcggtctg gggaaagcgc ggggtgctcc ggcgcgcccc cggcctcgag
      301 accgaaggcc tgtcgggggc cgccgcgtcc tccgtgcgcg taactgaacg actcgagcct
      361 gacgccgatg cccacgaacg cctcgacgag aagtatgggc attaccggag ggtgatcgac
      421 gcgctcgagg aagcgtggga agtctcaccg ggtgagccgg cttgatgcgt cggcggcgag
      481 acctgcgtgg gggagggcat tcggaatgaa agctgccgtg tttcacggac ccggggagat
      541 ggaggtaacg gaggtcgaca ggccagaggt cggcccggag gagatcctca taaaggtcgg
      601 tgccaacacc gtctgcggga ccgacgtgcg gatcgtgacg ggacagaaga ccagcggcgt
      661 gaggcggccc tcgatcatag gccacgagtt cgcgggaaag atcgcgcagg ttggcgagag
      721 ggtcaggggt tacgagcagg ggatgccggt cgccgttgcg cccaccatcc cgtgccacag
      781 gtgctactac tgccagcacg ggatggagaa cgtctgcgac gacaagcgcc gcctgggcta
      841 cgagctggac ggtggcctcg gtgagtacgt gctcgtcccg gccgacgggg tcagttcggg
      901 caacgttttc gtcgtcggag acgacacgcc cgtggaacac ctggcgctcg ccgagccgct
      961 cgcctgttgt gtgaacgggc agaggcagac gccggtcggg ctgaacgaca ccgtcctcat
     1021 catgggggcc ggcccgatcg gtctctttca cctgcagctg gcgctgttat ccggggccga
     1081 gaccgttatc gtgagccagc cgtcggcgcc acgccgggag ttcgcggccg gcctcggcgc
     1141 ccacctgacg gtgaaccccg gagaagaaaa cctctcctcc gtggtcgcgg aggctaccgg
     1201 ggggcttggt gtggacgtgg cgatcgtctg catcggaaaa cccgggctcg taaacgacgc
     1261 gctcgggctt gtccgaaggg ggggaagcgt caacatcttc gccggcctct cgggagaggg
     1321 ttgggcggag ctcgaggcga acaggatcca ctacgaccag ctcagggtca ccggcgtctc
     1381 cgactgtagc cgctccgatt acgaggccgc cctgcggctg atacggtcgg gcagggtgga
     1441 tgtgagcaag atggtgaccc accgcttccc cctcgctgag gtcaacgcag ccctcgattc
     1501 cacggccaac ggcgagggca taaaggctgc cgtcgtgccg taaacgtaac gaacttcgga
     1561 agtgagagca gggacgggga ggcctcccag ttgcaagggc ctgctcggag atagccagca
     1621 caggacgaga gaggggtcac agaatgggtc tcagggagaa actagaggta ctggagcgcg
     1681 aaggtacacc aatccggatc gggctcgtcg gggccgggca gatgggaagg ggcctcatcg
     1741 cccagatcgc aggcataccc ggcatggagg tgacggccgt cgcggacata gccccggagc
     1801 gggccatgag cgcgttccgg gagatcgggc aggaacccgt aaccgatcga gaggggcggc
     1861 cgggccgccc caacgtcacc gaagatgcgg gggacctctc ccactccgac accgtcgacg
     1921 tcgtggtaga ggcgacgggg gtgccggaga tcggggcccg cgtggcgttc gacgccatcc
     1981 acaacggcaa acacgtcgtc atgctgaacg tggaggcgga cgtgaccatc ggtcccatac
     2041 tgacccgcat gggccgctcc gccggggtgg tctacacggg ctcggccggt gacgaacccg
     2101 gcgccatcat ggagctctac gatttcgccc gctcgctcgg cttcgaggtg gtcgccgccg
     2161 gcaagggcaa gaacaacccg ctcgatttca cggccacccc cgactcgctg gccgacgagg
     2221 ccgccaccaa gcatatgaac cccaagatgc tggcctcctt cgtggatggc acgaagacta
     2281 tggtcgagat ggccgcggtc gcgaacgcca cgggcatgac cccggacaca cctggcatgc
     2341 acggccccga agagacggtc ccaaaccggc tggcggacct cttcagcctc gaaaaggagg
     2401 ggggcatact ctccagctac gacagcgtgg actacgtgcg gggcatcgcc ccaggcgtgt
     2461 tcgtcatcgt gcaggccgcg gctgagcccg tcagggagac cctggagtac ctgggccagg
     2521 gggccggtcc caatcacgtg ctgtaccgtc cgtaccacct cacgagcctg gaaaccccga
     2581 tctcggctgc ccgcgccgtg ctctacggag agccaacgat agcgtcacgc gacaacccct
     2641 cagccgaggt ggtcgcggtg gccaaacgcg acctcgaacc gggcgagacg ctcggcagca
     2701 tcggctctgc ggactactac acaagcatct acggtgttga tgaggcccgc gacatgctgc
     2761 cgatgggcct cgcggagggg ggcaggacga agcgtgccat agcgcaggga gacgtcatat
     2821 gccgggcgga cgtagagcag gcaagcggaa ccttcgtggg cgatctgagg cgtcttcagg
     2881 agacggccct gggatggaag agctagagag ccagacgggc tgaaagcgtc cctgctcgaa
     2941 aagcgggccg gagaggatcc ggcccgcctc taacctagcg gacctcgaag gtaagcgact
     3001 cggtcggctg ctcagcgtcg ttcaggtaga gttgcaccgc gtattctcct gtcggccagg
     3061 actcgcctgg gctcaggttg aagcggaaca ggccgtcgcc gcccgtggcg ctgatctgct
     3121 tgatcttgga gttcggttct atgttctcga tgtcggccgc gagccatacc gccgttatct
     3181 tcgtgtcatc gggagcgcct tcgaggtagc cgacacagta gaacgtgtct ttcggcgaga
     3241 acaccgtggt tggctggtcg ccgttctcgt cggccgccat cttcacgtcg ctcatggtga
     3301 tcgtgccggt gtcgtcggct ggcgcctcgg taggggtgct ctctggcgtg ctggtgggcg
     3361 tgctctccgg tgtattggcc ggcgccttgg cgggcggatt gtccttggtt gcgggctcgt
     3421 caccaccacc gctgagagcg acgatggcga ctatcgcgat gatgaggagc gccaacacgc
     3481 ctatgagccc caggcagccg gcgatgaaca ggttacgtcg tgaccggggg cgttgcgcgt
     3541 cttcggcttg ctgcggcgga ggggcggcgg ccgccgcggc ctgcggcggc gggggtggaa
     3601 cgggaacgtc ggcttcggga gtcggaacag tcgctgcctc gtgtacgggg ctcccgcaat
     3661 tggggcagaa ctggtcctcg gtgtccaact cgtgcccgca attactacag tacctatccg
     3721 gtgtctcagc catacgagtt cccttctacc tcgcagcaac ctgacgcggg gccgcaagca
     3781 aggcaacccc atcctgaacc tcttgcaggg tagtatgaac gaccacggcg cctggcgcat
     3841 tcgttctggt cgaaagctgc aggctctgat ctcttcgccc ggccctgacc ttgtgtactc
     3901 caggtgagtg tcccgtccgg gttcctggtc ccgaccgagg ggctcgacaa ctcaacggat
     3961 gcccgctcct ccctggacga gatccttgct ggcgtctact acctctcgca cggcgcgccc
     4021 atccgtcacg gttggctttc cgtcgccctc ctgcggcccg taacttccga actgggcatg
     4081 gttcatgccc tctatctcca tgtatccggt gagttcggga agacggctct tcgcctcttc
     4141 gaaagaccga cggtccagca ctctatcccg ggacccaagt atgctcgcga cctcgacctg
     4201 gtcattcgag agatccgtgc ccctgaagga tagcttgcga agagcagcag cccgtcgacg
     4261 gtctcgccgg ctctctcctc agcgtaggcc gcggccgcca ccccgccgag gctgtgtcct
     4321 gccagggtcc acctccggac ttcggggtgt gcagcgatga cctcgtcggc cgcgtcgccg
     4381 tcgaggaggg ccatgtcgag tgggacgcgc accacagcca ccatcgcgcc ggtctgttcc
     4441 gagagctcgg aggccagagg agcgtacgcc tcggcgtcca ccctcgcgcc gggatagagc
     4501 acgacccctg tgcagctttc cctgccaccg aattcgtacc accctccctc gtcggtagcc
     4561 ccggacatcg cagccacagc gacagcgtca ggtttatagg agccgaaagt ccaccaggtg
     4621 agcgcgcaga caacgaccgc cacgaccccc gaagctgcga caagcagtat ccgtgccagg
     4681 ttcattctgg agcgtattgt gcggtacttc cggcgttctg cgctactgga gcgtggcctg
     4741 cctgcttctc gccagcgaaa acgacgatcg cggagtagtc cgcatcggag cagggaagat
     4801 gcccgagcca tccgagaccc tgttcactgc aaagttgtgc gccgcgtgat ctaaaatcct
     4861 agagaacctg gcctggccga gtagcgttgc gtcgatgaca cgagccatgc cggaacgatc
     4921 ttctcgggtg gaggtacggt atgggcaagg cagcggaagc tccgactacg gcgaccctgg
     4981 actggtggcg gatcggcgtg gcgctgttcg gggcgttctt ccaggtgatc gtgggtttct
     5041 tcgtgccggt gggaagcctc gtgaacaggg aagtctcgct gatcatcccg gcgggatgga
     5101 cgtttgccat ctgggggccg attttcttgc tctgctttct cttcgccggg taccagttgc
     5161 tcccgtccag gcgctacgat ccacttctca gaagcgtggg atggccgctc gggctcgcct
     5221 tcgtgggcaa cggactgtgg accttgatac agccattcag gcagccgatc ctctcgcagg
     5281 tcgtcatctt cctcatcctg atcctcgccc tcacggcgct cgtcaggttt gccaggtttg
     5341 gtgcggtttc cccggtgcag cggtggatcg tcgggctccc cgtaggagcg ctggcggggt
     5401 ggctgacagc ggcgaacgtc gtcggcttca acgacatgct ggtcagggaa gacgtggtag
     5461 gctccagcgt tctggccgcc ctggtcggtg cgggtctgct cgtcgtcgga acgatcgtag
     5521 cggcacgggt gcttgctgtg agctttgccg gaccccttca ggcgagcatc gcgtacgcgg
     5581 cagcggtgct ctgggggctc ttcggcatcg ctgcgaacca gtacgactct tccgtgataa
     5641 ccacgctcac ctccgtcgtc tgcttcgtga cgctcgcata cttgtctgtc ctgacgctcg
     5701 tgagacggcg gggccatggt gcccccagag gtagggccgt ctgaagagaa gggtgcgcga
     5761 agccagggct ccgagcacat cgagccattg aagcaccact ggcaattggc taccataaac
     5821 gatatcgggc cgcgtctacg ccgggatcga accgccggca tctacaacga ataaccacga
     5881 ccgattggta ggtggagatg ttcgcagcct ggggacgttt cgtgtaccgc ttccggtggc
     5941 tggtgctctt cctgtccatc ctctccatcg cggccgtggc ctactctgcg cagtacggcg
     6001 gaaggctggg aaccggcgag ttcgcggccc ccgaaggcac ggagtcgggg cgggtcgacg
     6061 ttctgttgga cgaggaggtg gcgaagaccc cgccagcctt taccctcatc ttcggcagcg
     6121 acgaggagaa ggctaccgac cccgcgttcc gagacggggt ggagcgtgcg ctggggcccc
     6181 tcgaagacga cgatagggtg accaacgtcc gaaccgccta cgaaaacggc gccgtggacg
     6241 gggacatgat ctccacagac gagcatcaca gcctggtggt ggtggagctc gaggagggta
     6301 gctttaccga gcgtcaggat gcctacgtgg acctcaggca ggaggtgcgg gagggaccgt
     6361 tcgaggtcgt cgctgcgggg gacatgccgc tcaacgagga cttcgagagg gttacggaga
     6421 gagacctgaa gcgcgccgag gtggtctcct tgccgctggc cctcctgatg ctgctcttcg
     6481 tcttcggttc cgtggtcgcg gccggtttgc ctattggggt gggcatcctc gccgtcgccg
     6541 gcggcttcac ggggaccctc cttctcgcga ggctgatgga cgtctcgatc tacgcgacca
     6601 acatcgtcac catgatcggg ctcggcgtcg ccatagacta ctcgctcttc gtcgttagcc
     6661 gttttcgtga ggaggtcgga cgccaccccg ttcccgaagc gctggcacgt accatggcca
     6721 cggcgggacg cgccatactg ttctccgggc taaccgtggc aatcggcttt atgggcttcc
     6781 tgttcttcga cgttgccgat ctcaactcca taggcttcgc cggctccatc gtggtcgcct
     6841 tctcggtgct ctacgggctg accttcctgc cagcgctgct cgctatcctg ggtccccgcg
     6901 tggatttcct tcggctgccc ttcgtccgcc cggggagggt cgccacggga acgggcccgt
     6961 ggcacaggct ggcaaccgcc gtcatggccc acccgtggcg cacgctcctg cccgtcgtcg
     7021 cgctcctcct cgtgctcgga tcccctttcc tgaacatccg tctcggcctc tcgggcgctg
     7081 acacgctctc gagagactcc gagtcgcggc ttggagagga gcttctggac cgccagtttc
     7141 cggacaccag cgcctacccc atggtggtgg tgctcgacta ccctcagggg tcgtcgctca
     7201 gcaaggagcg ggtgggagag acgttcgacc tgagccgctg gctcgagcac aggaagggcg
     7261 tcaaagacgt ggagagcgtc gtgaacctcg gccccgggct aacgcgggac cagtaccagg
     7321 agcttctctc ggggccgcgc ggggaccttc cgccggagat acgaaacgcc ttgaaaagga
     7381 ctacagggga ccacctcacg gtcctgaccg cctacacctc ggttggctac aactcggatg
     7441 aggcgtacga cctggtacgc gaggtccgcg aacggcaccc cgacgtcggt ggtgaggtgc
     7501 tcgtcggggg ctgggcggcc ttcgacctcg acctcaccga atcggtctac gaagccgcgc
     7561 cgccggctat cgcgttcgtg gtcctggcca cctacgtggt gctcttcttg ctcctcggct
     7621 ccgtgctcct gcccataaaa gccgtggtgg tgaacttcct gtcgatcagc gcctcctacg
     7681 gggcgctcgt ctggg
//
LOCUS       DBNGVD010000440        11874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_650502, whole genome shotgun sequence.
ACCESSION   DBNGVD010000440 DBNGVD010000000
VERSION     DBNGVD010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_650502"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1248)
                     /gene="ispG"
                     /locus_tag="AC16CM_11880"
                     /gene_synonym="gcpE"
     CDS             complement(175..1248)
                     /gene="ispG"
                     /locus_tag="AC16CM_11880"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3140577.1"
                     /translation="MTEAAVRERPVTSRQITVGDVPIGGGAQVAVQSMTNTMTYDVEA
                     TVQQVYDLNAAGADLVRVSVNGSKALKGFREVVWRSPVPLIADIHFDYRMALGAADAG
                     AACVRINPGNVGNDDRYRQVIEKCQVTGTAMRIGVNSGSVEKKYWDLPKAEALVASAL
                     HKVEIAEEMDFFNFKVSLKASHVPEMVEANRKFREHSDAPLHLGVTEAGTRLPGAIKS
                     AAALGQLLPYGIGDTIRISLAEDPVEEIPVAYHILSALDLRHRGPNVIACPSCARTLG
                     FDVIGMAARVEEGLKPFEDHFTVAVMGCVVNGPGEARDADYGVAGGKDDGVIFSKGQP
                     LRKVGRDEIYDALFEEIAKDGHK"
     gene            complement(1279..2322)
                     /gene="rseP"
                     /locus_tag="AC16CM_11885"
     CDS             complement(1279..2322)
                     /gene="rseP"
                     /locus_tag="AC16CM_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3140578.1"
                     /translation="MTVIVALLGLILLIVIHELGHMLTAKALGVRVPEFAVGFGPALI
                     KKKFGKTVYSFRIILLGGFAKMAGMGDGEEGPDTYHAKPPWRRALIILAGPAANAVAA
                     VLILACVYMFQGVVTDVKPVVTEVEPRSTAQEVGIRPGDRIVELDGSPIKSWDGFQRN
                     MADREPGEQVGVTVVRDGEQKTFEGELGSDPQDNQQALLGVRPEVETTTYGPLQSLGH
                     AVERCVQITAKLGQFVGQLVTGEKDLYENVTGPVGITSVGSTAVSQGFFLPLLALISL
                     NLALFNLLPILPLDGGHLFFIAAEKLLGRPVSEQTMGKVAAFGLVLVLALFLFATYAD
                     LSKLFTGQPFIPD"
     gene            complement(2319..3452)
                     /gene="dxr"
                     /locus_tag="AC16CM_11890"
     CDS             complement(2319..3452)
                     /gene="dxr"
                     /locus_tag="AC16CM_11890"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3140579.1"
                     /translation="MRRRLTILGSTGSVGRQALDVVRQYPDRFEVVGLSAGSDAQSLA
                     QQANEFRPEYVALESTATEPLDGLDAELISGPGASEALARTSADVVLNSIVGFAGLAA
                     TVGALEAGNQVALANKESLVAGGEWVMNLANGNRIIPVDSEHSAIFQCLEGRQESEVR
                     EILLTASGGPFYAFEKERVVEAGPGDALKHPTWQMGPKITIDSATMMNKGLEVIEAHY
                     LFGVAYEEVRVVVHRQSVVHGGAMFEDGSVVLHAALPDMRLPIAFGVLYPDRVDVGAE
                     PVPFGGTSWTFEEPRNDLFRCLPLAVEAGRAGEAYPVALNAANEVAVGAFLDGRIGFL
                     RIADLIEEVLGEVPDFGKMQSMEKIRAVDAWAREDARVRVEAV"
     gene            complement(3472..4242)
                     /locus_tag="AC16CM_11895"
     CDS             complement(3472..4242)
                     /locus_tag="AC16CM_11895"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3140580.1"
                     /translation="MLRWRAVTALVLAPIALASIALGKWAILLVLLVVISGAAYELSR
                     ALEPLPFFAAFGAGALPLLLSIPYGRTGILLGAVIAMPWALFWLAARPETRTLRAVLA
                     VLLMSLWVGTPLAHLGIFPDTRDGVVLILIAVVGPWISDSGAYFAGRLFGRHLLFPSL
                     SPKKTVEGGLGGLLLTVLVVSPLAYFLLSFSPPKALLIGAAVSVASQCGDLFESSLKR
                     ILDVKDLGNFLPGHGGVLDRVDSLLFTAPAVYYIYLLV"
     gene            complement(4249..4917)
                     /gene="uppS"
                     /locus_tag="AC16CM_11900"
     CDS             complement(4249..4917)
                     /gene="uppS"
                     /locus_tag="AC16CM_11900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR3140581.1"
                     /translation="MAIIMDGNGRWAKRRWLPRAAGHRAGVSVLTPLLETAGQAGVDT
                     LTLYAFSTENWGRPEKEVSMLMKLFLETAREKVPELNERGARLRFLGRREGLPASVLE
                     ALAEAEDLTSDNDSLDVYIALNYGGRAEIVDATKRMMADGLAPEDVDEKAFARYLYAP
                     DVPEVDLVIRTSGELRVSNFLLWQIAYAEFYVTDTLWPDFSAEEFLRALESFASRSRR
                     RGGV"
     gene            complement(4999..5559)
                     /gene="frr"
                     /locus_tag="AC16CM_11905"
     CDS             complement(4999..5559)
                     /gene="frr"
                     /locus_tag="AC16CM_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3140582.1"
                     /translation="MSDVIDLSAAERRMGGAVDSVKSNFATIRTGRANPSLLDRIEVE
                     AYGTKMDLRSVANIGAPEARLLTVTPFDPNSLKDIERAIRDANIGLNPQNDGKILRLP
                     IPELTEERRRDLIRMARGMAEEGKVSVRNVRRDEMRDVHELRKEGEISEDDEHRAEEE
                     LQKLTNAYVARVEAALADKEAELSEV"
     gene            complement(5563..6321)
                     /gene="pyrH"
                     /locus_tag="AC16CM_11910"
     CDS             complement(5563..6321)
                     /gene="pyrH"
                     /locus_tag="AC16CM_11910"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3140583.1"
                     /translation="MDGAGLSELGPVNRVVLKISGEALAGDSDLGIEAASLDSVCAGV
                     MEAVEAGAELAVVVGGGNIFRGSSAARALGIESATADYMSMLGTVINAIALQAALEGR
                     GVETRVQSAVEIKEVAEPYIRRRAIRHLEKGRVVIFGGGTGNPFFTTDTGGALRALEI
                     GADALLMAKNQVDGIYDRDPRRHDDAVRLPRLNYMDLLSDNLSVMDHTAATLCSGENL
                     PIVVFDILKQGNLRRILRGEKVGSLVWGEASGGG"
     gene            complement(6362..6949)
                     /gene="tsf"
                     /locus_tag="AC16CM_11915"
     CDS             complement(6362..6949)
                     /gene="tsf"
                     /locus_tag="AC16CM_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3140584.1"
                     /translation="MASTIEKIKQLREETAAGMMDVKRALEESDGDVEGARQVLREKG
                     QALAQKKSGRAATEGLIEAYVHFNGRIGVLVEVNCETDFVARTPEFKTFARNVALHVA
                     SMRPMCVTPEDIPAGALEEERQVVEKQVAEMGKPDNIAEKIVEGRVNKWVSEQALTTQ
                     PYVKEPDKTVGDLLQETIQKVGENVVIRRFVHYEL"
     gene            complement(6955..8097)
                     /gene="rpsB"
                     /locus_tag="AC16CM_11920"
     CDS             complement(6955..8097)
                     /gene="rpsB"
                     /locus_tag="AC16CM_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3140585.1"
                     /translation="MQSTSERVGIRELLEAGVHFGHQAKRWNPKMKRYIFAQRTGVHI
                     IDLDQTLTLLGRALDFVRGVAEANREVLFVGTKKQAQIPLAEEALRSGQPFVAERYIG
                     GMLTNFQTILPRIQYFKELAVRVDDTPEEARSGKDWFALNREYQKLRRNFAGITEMER
                     LPGAVFVIDPKREELLVKEANRLKIPVLALTDTNCDPEVVDYVIPGNDDAIRSISLIS
                     RLVADSIIEGRGEEARVDERPVPPVIEDATVGGEVQQVETSDNGAQEPPSVEVTEEPP
                     PADVPEGGAEPATEPEAVAEPEAAAENGATDEPGAVVEAEIVGAPAGTEVADEEAAVT
                     EIDIESVEPEAAEELASEEPVEEDIEQAPVIEDPAQEATSENTEEK"
     gene            complement(8408..9253)
                     /locus_tag="AC16CM_11925"
     CDS             complement(8408..9253)
                     /locus_tag="AC16CM_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HIR3140586.1"
                     /translation="MTRRTLGELWSRYFRVRGRIAAGGMSCVQLRSAEREARGLRDSL
                     VVNYSPLVRYVAGRTAGRVGGALDLEDLISSGLVGLLGAVETFDPGRRTKFESYAISK
                     IRWSILDELRKADPLSRRTRHRVHQIDRARDELFQKLGRTPTESELVGRLGITVTEHR
                     EFLGQYARAQVRSFEGCGDLPGGPVGGLHDLIGDHAATDPASAAEVAEVRARLVSAIG
                     CLGEKERVVTTSYYFEGLTLREIGRVLGLTEGRISQILHASLARLRRSLDEDGVLPGR
                     TACDR"
     gene            complement(9250..10356)
                     /gene="dprA"
                     /locus_tag="AC16CM_11930"
     CDS             complement(9250..10356)
                     /gene="dprA"
                     /locus_tag="AC16CM_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3140587.1"
                     /translation="MEDSERRGERAKHLFLSHLQARVGSSLVARLGNLSPGAIFETPY
                     ADLVGKVGKDQKLERLFDGLQRRFDLALVEAQMKERGISAITLADEAYPGMLRNVPDP
                     PPALYLDGAIPEEPCVALVGSRKASATGIEMAGKLGRALAERGVCVVSGLALGVDAAA
                     HQGAVDAAGRTVGVLGCGIDVVYPRSNRALFDSVRRHGALVSEYFLGEAPLSWRFPAR
                     NRIIAGLVTTVVVVEAPEHSGALITARHGLEAGRDVWAVPGPPGVRECRGSNGLLADG
                     GGVLWDIPEFLEAVAPRGGAGPELPAPSVPADLPDDEAAALCGVGLEPRGVDAVARRS
                     GMVMSELLPALALLELKGFVHRDAVGAYVRRAVP"
     gene            complement(10346..11854)
                     /locus_tag="AC16CM_11935"
     CDS             complement(10346..11854)
                     /locus_tag="AC16CM_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3140588.1"
                     /translation="MSVCRARSLSLFGIDALPVEVEVDVSPGLPGFCIVGLPDAAVQE
                     ARERVRVAISNSGYKFPMKRVIVNLAPANLRKEGPAFDLPIALGILAASGALAPAGLE
                     RAAVVGELSLDGGVRGIKGALSMAEGARKEGLSRLVLPCRDAPEAAVIGGVEVFGMRT
                     LKEAVEFLGNGLRRAPTEPAPQNETDDSAADDFADVAGQRYAKRALEVAAAGAHNVVM
                     SGPPGSGKTMLARRMPGILPPLTMEERIEVTKVHSASGEGNGSLITHRPFRSPHHTVS
                     TSGLAGGGSNPRPGEVSLAHNGVLFLDELPEFGRTTLEALRQPLEDGHVTISRVAATL
                     CYPARITLLCSMNPCPCGHAGDKRKACRCTAGQIERYQARISGPLLDRMDIFVDVPRL
                     SRQELRDEPSAEASAVVRDRVRKARATQISRQGSPNSMLSGRKLRKMCVLAGDAETLF
                     SRAVETLGLSGRGHDRVLRVARTVADLAGERGIGADHLAEALNYRRSHLLGR"
BASE COUNT         2006 a         4112 c         3575 g         2181 t
ORIGIN      
        1 taccaatgca tcatactccg gccactgaac gaagacaacg gtctgagctg ggggtcggga
       61 ggagaccccg gcccacgagg tctcgaagcg cttcagaggg ttgggctgtc agccgttcct
      121 ggccacagtg gccccgcagg tatagtcgac gctctggctt gacgcccctt caggctactt
      181 gtggccgtct ttcgctatct cctcgaagag agcgtcgtag atctcatccc gccccacctt
      241 gcgcaggggc tggcccttgg agaatatgac gccgtcgtcc ttgccgccgg ctaccccgta
      301 gtccgcgtcg cgcgcctcgc ccggcccgtt gaccacacaa cccatgacgg cgaccgtgaa
      361 gtgatcctcg aagggtttca ggccctcttc gaccctggcg gccatcccga tcacgtcgaa
      421 gccaagcgtg cgggcgcaac tcgggcaggc gataacgttc gggccgcgat gcctgaggtc
      481 gagggctgac aggatgtggt aggcgaccgg tatctcttct accggatcct cggcgagcga
      541 gatgcggatg gtgtccccta tgccgtaggg gaggagctgc cccagcgccg ccgcgctctt
      601 tatggcgcct ggaagcctgg tcccggcttc ggtaacgccg aggtgcaggg gggcgtcgga
      661 gtgctcgcga aacttcctgt ttgcctcgac catctccggg acgtggctgg ccttcagcga
      721 gaccttgaag ttgaagaagt ccatctcctc ggcgatctcc accttgtgca gggcgctcgc
      781 cacgagcgcc tccgccttcg gaagatccca gtactttttc tccacagacc cggagttcac
      841 gccaatgcgc atggccgtac cagtcacctg gcacttctcg atgacctgcc gatagcggtc
      901 gtcgttgcca acgttaccgg ggttgatacg tacacaggcg gcgcccgcgt cagcggcgcc
      961 gagcgccata cggtagtcga agtggatgtc cgcgatcagg ggaacggggg agcgccacac
     1021 gacctccctg aagcccttca gcgccttcga cccgtttacc gagacgcgca cgaggtcagc
     1081 cccggcggcg ttcaggtcgt agacctgctg caccgtggcc tccacgtcgt aggtcatcgt
     1141 gtttgtcatc gattgcacag ccacctgggc cccgccaccg atgggcacgt cgccgaccgt
     1201 gatctgccgg ctcgttaccg gccgttctcg tactgctgcc tctgtcatct cactcccacg
     1261 aatcgtctca aaagtattct aatccggtat gaagggttgc ccggtgaaca gtttactcag
     1321 gtccgcgtac gtcgcaaaca ggaacaacgc aaggaccagc acgaggccga aggctgcgac
     1381 cttgcccatc gtctgctcgc ttaccggcct gcccagcaac ttctcggccg ctataaagaa
     1441 gaggtgacca ccgtcgagcg gcagtatggg aagcaaattg aacagcgcga ggttgaggct
     1501 gatcagggcc agaagcggca ggaagaatcc ctggcttacg gccgtgctcc cgaccgaggt
     1561 gatgcccacg gggcccgtaa cgttctcgta gaggtccttc tctccggtga cgagctgccc
     1621 gacgaattgc ccgagcttgg cggttatctg cacgcacctc tccaccgcgt ggccgagcga
     1681 ctgcagggga ccgtaggtgg tggtctcgac ctccggcctt acccccagaa gcgcctgctg
     1741 gttatcctgc ggatcgctcc ccagttcgcc ctcgaaggtc ttctgctcgc cgtcacgaac
     1801 cacggtcacg ccgacctgtt cgccgggctc tctatccgcc atattccgct gaaacccgtc
     1861 ccagctcttt atcgggctac catcgagctc cacgatccgg tcccccggcc tgatcccgac
     1921 ctcctgggcc gttgaacggg gctctacctc ggttactacg ggcttgacgt cggtgacgac
     1981 cccctgaaac atgtagacgc acgcgagtat gaggacggcc gcgaccgcgt tggccgccgg
     2041 ccctgccaga atgatcaggg cccgccgcca cgggggcttg gcgtggtagg tgtccggacc
     2101 ttcctccccg tctcccatgc cggccatctt ggcgaacccg cctaggagga ttatccggaa
     2161 cgagtagacg gtcttgccga acttcttctt gatcagggcc ggcccgaagc caacggcgaa
     2221 ctcgggtacc ctgacgccga gggccttggc cgtgagcatg tgacctagct cgtgaataac
     2281 gattaggagt atcagaccta gcagcgcgac gattacggtc ataccgcttc aacccgtact
     2341 cgggcatcct cacgcgccca cgcgtccacg gctcttattt tctccatgct ttgcatcttg
     2401 ccgaaatcgg gcacttcccc gaggacctcc tcgatgagat ctgctatccg caagaatcct
     2461 atcctgccgt caaggaaagc cccgacggcg acctcgttgg cggcgttgag cgccaccgga
     2521 tatgcctcgc cggcgcggcc agcctcgacg gcgagaggca gacacctgaa caggtcgttt
     2581 cgcggctcct cgaaggtcca actcgtcccc ccgaaaggca ccggctcggc cccgacatct
     2641 accctatccg gatacagtac cccgaacgcg atgggtaacc tcatgtcggg aagcgcggcg
     2701 tgcaacacga ccgagccgtc ctcgaacatc gcgcccccat gtactaccga ctgacggtgc
     2761 acgacgacgc gcacctcctc gtaggctacg ccgaacaggt aatgagcctc tatgacctcg
     2821 agacccttgt tcatcatggt ggccgagtct atcgtgatct tcggccccat ctgccacgtc
     2881 ggatgcttga gcgcgtcccc cggtcccgcc tccacgacac gctccttttc gaacgcgtag
     2941 aaaggccccc ccgacgccgt caacaagatc tcccgcacct ccgactcctg cctaccctct
     3001 aggcactgga agatggcgga atgctcggag tctacaggta ttatgcggtt gccgttggca
     3061 aggttcatga cccactcgcc tccggccacg agcgattcct tgttggccag ggctacctgg
     3121 tttccggcct cgagcgcgcc cacggtggcc gcgagtcctg cgaagccaac tatgctgttg
     3181 agcaccacgt cggcggacgt tctggcgagg gcttccgatg cgcccgggcc ggagataagt
     3241 tccgcatcga ggccgtccag cggctccgta gccgtcgact caagggcaac gtactctggc
     3301 ctgaactcgt tcgcctgttg tgcgagcgac tgtgcgtcgc ttcccgcgga gaggccgacc
     3361 acttcgaagc ggtccgggta ctggcgcact acgtcgaggg cctggcgccc gacgctaccg
     3421 gtcgagccaa ggatcgtgag tcgtcgtctc atggttgtct gggttccaat ctcatacgag
     3481 caagtagata tagtataccg ccggggccgt gaacagcagg ctgtccactc ggtcaagtac
     3541 gcccccgtgc cccggcagga agttgccaag atccttcacg tcgaggatac gtttcagaga
     3601 agactcgaac aggtcgccgc actggctcgc aacggagacg gcggccccta tgaggagcgc
     3661 cttgggtggg ctgaacgaca gaagaaagta ggcgagcggg gagaccacga gcacggtcag
     3721 cagcaggccg ccgagcccac cctcaacggt cttcttcggg ctcaggctcg ggaagaggag
     3781 gtgccgcccg aaaaggcgtc cggcgaagta ggcgccggag tcggagatcc agggtcccac
     3841 aaccgctata aggatgagga cgacgccgtc gcgggtatcg gggaagatcc cgagatgcgc
     3901 caatggagta ccgacccaca acgacatcag cagaaccgcg agcacggccc tgagcgttcg
     3961 cgtctcgggc cttgcggcga gccagaacag cgcccagggc attgctatca cggccccaag
     4021 caggattccc gttctcccgt aagggatgga gaggagcaga ggtagggccc cggcgccgaa
     4081 cgcggcaaag aagggcaggg gctcaagggc ccgcgagagc tcgtaggccg cgcccgatat
     4141 caccaccagc aacacgagga ggatggccca tttcccgagg gctatgctgg cgagcgcgat
     4201 cggggcgagc acaagcgccg ttaccgccct ccagcgaagc atgcccgact agacgccccc
     4261 ccggcgccgc gatcgggacg cgaacgactc gagcgcccgc aagaactcct ccgccgaaaa
     4321 atccggccag agcgtgtccg tcacgtagaa ctcggcgtag gcgatctgcc agagcaagaa
     4381 gttggagacg cgaagctccc cgctggtccg gatcaccagg tcaacctccg gcacgtcagg
     4441 cgcgtacagg tagcgcgcga aggccttctc gtccacgtct tcgggcgcga ggccatccgc
     4501 catcatcctt ttggtcgcgt ccacgatctc tgcccgccca ccgtagttga gcgcgatgta
     4561 cacgtccagg ctatcgttat ccgaggtcag atcctctgcc tcagccagcg cttcaagcac
     4621 cgaagccgga agcccttcac gcctgcccag aaaacgaagc ctggcgcccc gttcgttcaa
     4681 ctcgggtacc ttctcgcgcg ccgtctcgag gaagagcttc atcagcatgc ttacttcctt
     4741 ttcgggacgt ccccagttct cggtcgagaa ggcgtagagg gtcagggtat ccaccccggc
     4801 ctggccagcc gtttcgagca agggggtcag tacggagaca ccagccctgt ggccagcggc
     4861 ccgcggaagc cagcggcgct ttgcccagcg cccgtttcca tccatgatga tggccacgtg
     4921 gctaggggtc agagccgggt cgagggttgg cctagcaggg gtttccgtgg gcaggcgtct
     4981 ctcggcaggt ttcagcagtt agacctccga gagctcggct tccttgtcgg cgagggccgc
     5041 ctcgaccctc gcgacatagg cgttcgtaag cttctgcagt tcctcctcgg ccctatgctc
     5101 gtcgtcctcg gagatctcac cttccttgcg aagctcgtgg acgtcgcgca tctcatcccg
     5161 gcgcacgttc cttaccgaga ccttgccctc ctccgccatg ccgcgtgcca tacggatcag
     5221 gtcccggcgc cgctcctctg tgagctcggg gatgggaagg cgaagaatct tgccgtcgtt
     5281 ttgcgggttg aggccgatgt tggcgtccct tatggcccgc tcgatatctt tgagcgagtt
     5341 cgggtcgaag ggggttacgg tgaggagccg ggcctcgggc gcgccgatgt tggccacgct
     5401 tctgaggtcc atcttggtac cgtaagcctc gacctcgatc ctgtcgagga gtgaggggtt
     5461 ggccctgccg gtccttatcg ttgcgaagtt gctcttaaca gagtcgacgg ccccgcccat
     5521 cctgcgctct gcagccgata gatctatcac gtcagacatc gctcagcctc ctccgcttgc
     5581 ctctccccat accaaagatc ccaccttttc accccgtagg atgcgccgca ggttcccttg
     5641 cttcagtata tcgaagacga ctatgggaag gttctccccg ctgcagagcg tggccgctgt
     5701 atggtccatg accgacaggt tgtcggagag gaggtccatg tagttcagac ggggcagacg
     5761 caccgcgtca tcatgccgcc tcgggtccct gtcgtagata ccgtctacct ggttctttgc
     5821 cattaggagc gcgtcggctc ctatctccag cgcccgcagc gcgccgcccg tgtcggtggt
     5881 gaagaatgga ttgcccgtgc cgccgccgaa gataacaacc cgccccttct cgaggtggcg
     5941 gatggcgcgc cgcctgatat acggctcggc gacctccttg atctctacgg ccgactgaac
     6001 cctcgtctcc acgccacggc cctccaacgc agcctgcaac gctatggcgt tgatcacggt
     6061 ccccagcatg gacatgtagt cggcggtggc gctctctatg ccaagcgccc gcgccgccga
     6121 ggagccgcgg aagatgttcc ccccgccaac gaccacggcg agctccgctc cagcctccac
     6181 agcctccatc accccggcgc acacggaatc gaggctcgct gcctctatcc ccaggtccga
     6241 gtccccggcg agtgcctcgc cggagatctt gagcaccacc cgatttacgg ggccaagctc
     6301 cgagagcccg gccccatcta tctcacccac tggtctggca gaatcttcca cggccctgcc
     6361 gctagagctc gtagtggacg aaacgccgaa tcacgacgtt ctcgccgacc ttctggatgg
     6421 tctcctgcaa cagatcgccg acggtcttgt cgggctcctt gacgtagggc tgggtggtga
     6481 gcgcctgctc ggagacccac ttgttcaccc tgccctcgac gatcttctcg gcgatgttgt
     6541 cgggcttgcc catctccgcg acctgcttct cgacgacctg ccgctcctcc tcgagtgcgc
     6601 cggccgggat gtcctcgggc gtcacgcaca tgggccgcat ggaggccacg tgcaacgcga
     6661 cgtttcgggc gaaagtcttg aattcggggg tgcgcgccac gaagtccgtc tcgcagttaa
     6721 cctcgacgag cacgccgatc cgcccgttga agtgaacgta ggcctctatg agcccctccg
     6781 tcgccgcccg tccgctcttc ttctgggcca gcgcctggcc cttctccctc aagacctgac
     6841 gggcaccttc cacgtccccg tctgactcct cgagggcccg cttcacgtcc atcatccccg
     6901 cggcagtctc ctcccggagc tgcttgatct tctctatcgt gctagccaat gttcctactt
     6961 ctcctcggtg ttctcgctcg tggcttcctg cgccggatcc tctatgaccg gcgcctgctc
     7021 tatgtcctct tctacgggtt cctccgacgc gagctcctcc gcggcctccg gctcgacaga
     7081 ctctatatct atctccgtca ccgcagcctc ttcgtcggca acttcggtcc ccgccggcgc
     7141 cccgactatc tcggcctcga ccacggcgcc gggctcatcg gtggccccgt tctcggctgc
     7201 ggcctccggc tctgcaacgg cttccggctc ggtggcgggc tcggctccac cctcgggcac
     7261 atcggcgggc ggtggctcct ccgttacctc cactgatggt ggctcctggg ccccgttgtc
     7321 ggaggtctcg acctgctgga cctcaccgcc gaccgtggcg tcttcgatta ccggtggcac
     7381 cgggcgttcg tcgacccggg cctcctcgcc cctgccctcg atgatggagt cggccaccag
     7441 gcgggagatg aggctgatcg agcgtatcgc gtcatcgttg ccgggaatga cgtagtcgac
     7501 gacctcgggg tcgcagttgg tatcggtaag cgccaggacc ggaatcttga gcctgttggc
     7561 ctccttgacg agtagctcct cgcgcttggg atctatgacg aagaccgcac cggggagacg
     7621 ctccatctcc gtgatgccgg cgaagttgcg tcggagcttc tggtactcgc ggttgagggc
     7681 gaaccagtcc ttgccggacc tggcctcctc gggcgtgtcg tcgaccctga ctgccagctc
     7741 cttgaagtac tggatgcgcg gcagaatggt ctgaaagttg gtgagcatac cgccgatgta
     7801 gcgctccgcc acgaaaggct gcccgctccg cagcgcctcc tcggccaggg ggatctgagc
     7861 ctgcttcttg gtccccacga agagtacttc gcggttcgcc tcagcgacgc cgcgcacgaa
     7921 gtccagagcc cgtcccagca aggtcagggt ctggtccagg tctatgatgt gcacccccgt
     7981 ccgctgggcg aagatgtacc gcttcatctt ggggttccaa cgcttggcct ggtgcccaaa
     8041 atggacgccg gcctctagca actccctgat tcctacgcgc tcgctcgtgc tctgcaagat
     8101 ctctacctct cttttcaggt ggttggtatc ctccgcccgc gcctcgcaat ccacccggca
     8161 agctctgggg cggaccacca cccgggtcct gaggacccta cggcgggcgt gtgtagtcac
     8221 ggacgcgccg cgaacacacg gccgcatccg aagcaagatc ttaacacagg cacatctatg
     8281 cttctatggc ggttcgggaa aaggcttcgg gcagcaggca tcaggcttca gagtcgcgtt
     8341 ccgaggccct ttgcctggtg ccccaagccg ctattcattc caggttcgct cctgccggag
     8401 caccgtccta tcggtcgcag gcggtccgtc ctgggagtac cccgtcttcg tccaggctgc
     8461 gcctcaggcg ggccagggag gcgtggagta tctgcgagat gcggccctct gtgaggccga
     8521 gtaccctgcc gatctccctc agcgtaagac cctcgaagta gtagctggtg gtaactaccc
     8581 gctctttttc accgaggcat cctatggcgc tgaccaggcg cgcccgcacc tcggccacct
     8641 ccgcggcgga ggccgggtcc gttgcggcat ggtcgccgat caggtcgtgc agcccgccga
     8701 ccggtccccc cggcagatcc ccacaaccct cgaaggacct gacctgggcc ctggcgtact
     8761 ggcccaggaa ctcgcggtgc tctgtgacgg taatgccgag tcgcccgacg agctcggact
     8821 cggtcggcgt tcggcccagc ttctgaaaca gctcgtccct tgcgcggtcg atctggtgca
     8881 ccctgtgtcg tgtgcggcgg gagaggggat cagctttccg tagctcatcc aggatggacc
     8941 atctgatctt ggagatggca tacgactcga acttggtcct gcgcccggga tcgaaggtct
     9001 cgaccgcccc aagcagccct acgagccccg aggagataag gtcttccagg tccagagccc
     9061 cacctacgcg tccggcggtc cttcccgcta cgtagcggac caggggcgag taattgacta
     9121 ccaggctgtc cctgagcccc cttgcctcac gctccgccga cctgagctgg acgcaggaca
     9181 taccgccggc ggcgatccgc ccgcggacgc gaaagtacct ggaccacaac tccccgagcg
     9241 tgcgcctcgt catggaaccg ccctcctcac gtatgcgcca acggcgtctc gatgcacgaa
     9301 tccctttagc tcgagcaggg caagggccgg aagcaactcg gacatgacca tcccactgcg
     9361 cctggcgacg gcgtcgacgc cccgtggctc gagccccacc ccacaaagcg cggcagcctc
     9421 gtcatctggc agatccgcag gtacgctggg ggccgggagc tctggaccgg ccccgccccg
     9481 aggtgcgacg gcctccagaa actcggggat gtcccacaac acgcccccgc cgtcggccag
     9541 caaaccgttg gagccccggc actcgcggac gcccggtgga ccaggcacag cccacacgtc
     9601 cctccccgcc tcgagaccgt gacgcgcggt tatcagggcg ccactgtgct cgggcgcctc
     9661 gacgacgacc acggtagtta caaggccggc gatgatcctg tttcgagccg gaaaccgcca
     9721 ggagaggggc gcctcaccga gaaagtactc ggagacgagc gcgccgtgac gccgcacgga
     9781 atcgaagagg gccctgttgc tccttggata gacgacgtcg atgccgcacc ccagaacccc
     9841 gacggtccgt cccgcggcat ccacggcgcc ctggtgcgcg gcggcgtcta cccctagcgc
     9901 gagaccgctc acgacgcaaa caccgcgctc cgccagggct ctcccgagct ttccggccat
     9961 ctcgataccg gtcgccgagg cttttcgaga cccgacgagc gcgacgcatg gctcctccgg
    10021 gatggcacca tccaggtaga gcgccggcgg cgggtctggc acgttcctga gcatcccggg
    10081 ataggcctcg tcggcgagcg tgatggccga gatcccccgc tctttcatct gggcctcgac
    10141 cagggccagg tcgaagcggc gctgcaaccc gtcgaagagg cgttcgagct tttgatcttt
    10201 cccgacctta cccaccaggt cggcgtacgg cgtctcgaag attgccccgg gggagaggtt
    10261 acccagcctc gcgacgaggc tgctcccgac cctggcttgc aggtgcgaga gaaacagatg
    10321 ctttgcacgc tcaccacgcc gctcgctatc ttccaaggag gtgagacctt ctgtagttga
    10381 gcgcctcggc caggtggtca gcaccgatac ctcgctcccc ggcaagatcc gccaccgttc
    10441 tcgccacccg caacaccctg tcgtgtcccc ttccggagag ccccagagtc tccaccgccc
    10501 gcgaaaagag cgtttcggcg tcgccagcca gcacgcacat ctttcgcagt ttgcgtccag
    10561 acagcatgga gttgggagag ccctgacggg atatctgcgt tgcccgtgct tttcggaccc
    10621 gatccctgac cacagcagaa gcctcggccg atggctcgtc gcgcagctcc tgccgcgaga
    10681 gccgcggcac gtctacgaag atgtccatcc ggtcgagcaa cggcccggat atgcgggcct
    10741 ggtagcgctc gatctggccg gcggtacacc ggcaggcctt cctcttgtca ccagcgtgcc
    10801 cgcaggggca cgggttcatg gaacagagca gcgtgatcct cgccgggtag cagagcgtgg
    10861 ccgccacgcg tgagatggtg acgtgaccgt cttcgagggg ctggcgcagg gcctccaacg
    10921 tggtcctgcc gaactcgggt aactcgtcga ggaacagcac cccgttgtgc gcgagcgaca
    10981 cctcacccgg ccgtgggttg ctgccgcctc ccgcgagccc cgaggtagag acggtgtggt
    11041 gcggcgacct gaagggcctg tgcgttatga gcgacccatt gccctcgccg ctcgcgctgt
    11101 ggaccttggt cacctcgatg cgctcctcca tggtcagcgg gggtaggatc cccggcatcc
    11161 gcctcgcaag catcgtcttg cccgaacccg gcggaccgct cataacgacg ttgtgggctc
    11221 cggcagcggc tacctcgagg gcacgcttgg cataccgttg tcctgcaacg tcggcgaaat
    11281 cgtccgccgc ggaatcgtcg gtctcgttct gtggcgcagg ctctgtgggt gcccggcgca
    11341 ggccattgcc gaggaattca acggcctctt tcagcgtcct catgccgaac acctcgacgc
    11401 cgccgatcac ggccgcctca ggagcgtccc tgcacggaag cacgagacgg ctcagcccct
    11461 ccttcctggc accttcggcc atggagaggg cacccttgat gcccctgacc ccgccgtcaa
    11521 gcgagagctc acctaccacg gcggcccgct ccagacccgc aggcgccaga gcccccgagg
    11581 ccgcgaggat gccgagcgct atgggtaggt cgaacgcggg cccctccttc ctgaggttgg
    11641 ccggggccag gttcacgatc acccgcttca ttggaaactt gtaaccgctg ttggagatgg
    11701 ccacccgtac acgctcccgt gcctcctgca cggccgcatc tggcaacccg acgatgcaga
    11761 accccggtaa cccggggctg acgtctacct ccacttcgac cggcagcgcg tcgataccga
    11821 agaggctcag gctcctggcc cggcatacgc tcatacttga cattccccca cagc
//
LOCUS       DBNGVD010000441         3964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1198535, whole genome shotgun sequence.
ACCESSION   DBNGVD010000441 DBNGVD010000000
VERSION     DBNGVD010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1198535"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..639)
                     /locus_tag="AC16CM_11940"
     CDS             complement(187..639)
                     /locus_tag="AC16CM_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140589.1"
                     /translation="MRVFLLVAAVTVGVANATLYSFIGNNTFDNLFEWQRDPWSLYML
                     YAFTAIFVGLLASGTLLRFAEGVLRDTFFARYGLMVLTICVGGAVLAVFLTTVSFMFD
                     PAAVVPTRIFQTLYTLGMVMVPGAILGATEGVVLAFPLAWMLGFFKKP"
     gene            complement(854..2104)
                     /locus_tag="AC16CM_11945"
     CDS             complement(854..2104)
                     /locus_tag="AC16CM_11945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3140590.1"
                     /translation="MQQRVNPGNKSLSDYRSIIRRELYDEVVTLGERLRGKRVLHVSA
                     TSFGGGVAEILYTMVPLMRDVGIDAEWDIMFGSEPFFNVTKSFHNALQGAEYELTVED
                     RAIFEEYNRMSAEAMQKAGEEWDIVLVHDPQPALLKHFTGGLSPETKWIWRCHPDLST
                     PNREVSDYLVPHIADYGAQVYTMQEYTPPDVHLPGLTLIPPAIDPLSPKNMALSAEDS
                     RYIVNQFGVDVERPFIVQVSRFDPWKDPLGVIDAYRMVKEQVPEVQLVLIGSMAHDDP
                     EGWDYWYKTVNYADGDRDIFLFSNLTNVGAIEVNAFQSIADVVIQKSIREGFGLVISE
                     ALWKARPLVAARVGGIPMQVDGGGGILIDTIPEAADACVKILKDPEFARQMGRLGKEH
                     VRDNYLSPRELRDHLLLFAQLLGV"
     gene            complement(2229..2675)
                     /locus_tag="AC16CM_11950"
     CDS             complement(2229..2675)
                     /locus_tag="AC16CM_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140591.1"
                     /translation="MERETAKATEEGSEFSAPIALNLPSSPEYVLLARLVMGQAGRLA
                     GLEPEEIYDLKLAVTEAVTNVIRHASVKNYEIEYRILPSGVEVSVTDDGGGFSVADLT
                     GEPDEHGGFGLAVIRNLVDELVLDSTRGGGTRLKMIRRAGTPADAG"
     gene            complement(2945..>3964)
                     /locus_tag="AC16CM_11955"
     CDS             complement(2945..>3964)
                     /locus_tag="AC16CM_11955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3140592.1"
                     /translation="ERAARLAQQRAARQEAREEARRQAELAAQQAAERRAAERAAERE
                     AERAAERAAIEEAAAEQAAAERAAAERAAAERAAAERAAERRADRQAAKEAELAAEAS
                     ASAAAAERADERAAERAAVRAAEQAAERAAEQAAERAAEASASAAAAEEDAERDARRE
                     AKAAEASASASASASASASASSQDEDNSGGGGGNQASASSSASASASAAPGGGGSSAL
                     GNDVLAVAQEHLGTPYVLSTPGPCEAFVAEDCSCFTMLVYQEFGIALPDSPGGQMGYG
                     VPVAGAPQAGDLLFWSEDGSGYITHVGIAMGDGTTIHASSYAGEVTITSINYISGYVG
                     ARRLL"
BASE COUNT          685 a         1324 c         1237 g          718 t
ORIGIN      
        1 cctccccgag agtgccatgt tcctcggcga tgacaccacg tacctcgacg ccttccgcga
       61 gctgtaaaac ctgagcgatg aaggacgact gagggatatc ccgcgcatag gcgtatcgag
      121 cgaagaaggc ccccctgaga taagaacccc gaggcagaca tcgtggtgga gggcgtaggc
      181 gaggtgctag ggcttcttga agaagcctag catccatgcc agggggaagg ccagcacgac
      241 gccttcagtg gccccgagta tggctcctgg gaccatcacc atgcccaggg tgtagagtgt
      301 ttggaagatc cgggtcggga cgaccgcggc agggtcgaac ataaagctta cggtggtcag
      361 gaagacggcc agcaccgcgc ccccgacgca gatagtaagt accataaggc cgtagcgggc
      421 gaagaaggta tcgcgcaaga ctccttcggc aaacctcaga agcgtgcccg aggccaggag
      481 ccccacgaag atggcggtga acgcgtacag catgtagagg gaccagggat cgcgttgcca
      541 ctcgaaaagg ttgtcgaagg tgttgttgcc gataaagctg tagagggtcg cgttcgcaac
      601 cccgaccgtc acggccgcga ccagcaagaa gacgcgcatc agaggttacc catgctgctc
      661 ccgatacgac tcgacctggt cctctgtgaa gtcggacgcg accgcctcag ccgaggcgcc
      721 ccggacggcg ttctccccgt ggaacgctca cgcccacccg actcgttccg gaggaacgta
      781 acgggtcccc agttcgaggt cacgatggta ctcccgcctt cgctcaaggt gtcccccctc
      841 ttacgcctac tgcttacacc cccagcagtt gggcgaagag aaggaggtgg tcgcgcagct
      901 cacgcgggct caggtaattg tcccgcacgt gctccttgcc cagacgaccc atctggcggg
      961 cgaactcggg gtccttcagg atcttgacgc aggcatcagc agcctcgggg atggtgtcta
     1021 taaggatgcc gcccccgccg tccacctgca tggggatgcc gcccaccctc gccgccacca
     1081 gcggcctagc cttccacagg gcctcgctga ttaccagccc gaagccctcg cggatggact
     1141 tctggatcac cacgtccgct atggactgga aggcgttgac ctcgatcgcc cccacgttgg
     1201 tgaggttgga gaacaggaag atgtccctgt cgccgtcggc gtaattgacc gtcttgtacc
     1261 agtagtccca gccctccggg tcgtcgtggg ccatcgagcc tatgaggacc agctggacct
     1321 cggggacctg ctccttgacc atcctgtagg cgtctatcac gccgagcggg tccttccagg
     1381 ggtcgaagcg cgacacctga acaatgaagg gcctctccac atccacgcca aactggttca
     1441 cgatgtagcg ggagtcctcg gcgctcaggg ccatgttttt gggcgagagt gggtctatgg
     1501 caggcggtat caaagtcagg ccaggcaggt gaacgtccgg cggggtgtac tcctgcatgg
     1561 tgtagacctg ggcgccgtag tcagcgatgt ggggcaccag gtagtcggag acctcgcggt
     1621 tgggcgtcga gaggtcgggg tggcagcgcc agatccattt ggtctcgggc gagaggccac
     1681 ctgtgaaatg cttgagcagc gccggctgcg ggtcgtgcac gagcacgata tcccactcct
     1741 cgcccgcctt ctgcatagcc tcggccgaca tgcggttgta ctcctcgaag atagcccggt
     1801 cctcgaccgt gagctcgtac tccgcgccct gcagggcgtt gtggaaactc ttggtgacgt
     1861 tgaagaacgg ctccgagccg aacatgatgt cccactcggc gtcgattcca acgtcccgca
     1921 tcaggggcac catcgtgtac agaatctccg cgaccccgcc gccgaagctc gtcgcgctga
     1981 cgtgcaggac acgcttgcct ctcagacgct ccccgagcgt tactacctca tcgtacagct
     2041 cacgacgtat gatgctgcga taatcggaga gcgatttgtt gcctgggttt acacgctgct
     2101 gcactgtcca tcctctggta tctttcggcg tcaggttcca acattaacct atttggttaa
     2161 ctgtatatta acgtcgactg aagaatcaac cggatcaggc tcaaaacagc gcaagttctg
     2221 gaattttctc agccggcatc cgcgggggtg cccgcgcgcc ggatcatctt caaccgtgtc
     2281 ccccctcccc tagtcgagtc gagcaccagc tcgtcgacga ggttgcggat cacggcaagg
     2341 ccgaaaccgc catgctcgtc gggctcgccc gtaaggtcgg cgacgctgaa gccgccgccg
     2401 tcatccgtga cgcttacctc tacaccggaa gggaggatcc tgtactcgat ctcgtaattc
     2461 ttcacggagg cgtgacggat cacgttcgtc accgcctcgg taacggccag cttgagatcg
     2521 tagatctcct ctggctcgag gccggccagg cgtccagcct gtcccattac gagcctggcg
     2581 agcaatacgt actcggggct gctcggcagg ttcagcgcaa tgggagccga gaactcgctc
     2641 ccctcttccg tcgccttggc tgtttcacgc tccatcggac ctctcggtca tctggctccc
     2701 tcacgcaaac cgatgagata gcttggcgga agcgcctctt tccaacaacg cgaaacttct
     2761 tacccagatg tatacgacgc taacccggcg tctgcgcggc ctggcccggg aggacgtcag
     2821 acgccagacg ggattccctc agattcgccc ggtaaaggac cagatccctt catcagagaa
     2881 acggccccag acggaaggag gggccccgtc taggcccctc gggttcgcgg gtgcgagcag
     2941 ccttctagag gagccttctg gcccccacgt agccgcttat gtagttgatg ctggtgatgg
     3001 tgacctcgcc cgcgtagctc gaggcgtgta tcgtggttcc gtcgcccatg gctatgccca
     3061 cgtgggtaat gtacccgctg ccgtcctcgc tccagaacag aaggtccccg gcctgtggtg
     3121 ccccggcgac aggtacaccg tagcccatct gcccgccggg cgagtcggga agggcgatgc
     3181 cgaactcctg gtagacgagc atggtgaagc aggagcagtc ctccgccacg aaggcctcgc
     3241 agggccccgg cgtcgagaga acgtaaggcg tccccagatg ctcctgggcc acggccagaa
     3301 cgtcgttgcc cagggcgctg gacccgccgc cacctggggc ggccgaggct gaggcgctgg
     3361 cagaggagga ggcagaagcc tggttcccgc cgccaccccc cgagttatcc tcgtcctggg
     3421 aagatgcgct ggcagatgcg ctggcggacg cgctggcaga cgcactcgcc tctgccgcct
     3481 tcgcttcccg cctggcgtca cgctcggcgt cctcctccgc ggcagcggcg ctcgcgctcg
     3541 cttcggcggc acgctcggct gcctgctcag cggcacgctc ggctgcctgc tcagcggcac
     3601 gcacggctgc ccgctcggct gcgcgctcgt cggccctctc cgccgcggcg gcgctcgcgc
     3661 tcgcttcggc ggccagctcc gcttcctttg cagcctgcct gtccgcgcgg cgttcggcgg
     3721 cacgctcggc tgcggcacgc tcggctgcgg cacgctcggc agcggcacgc tcggctgcgg
     3781 cctgctccgc cgcggcctcc tcaatagcgg cacgctcggc ggcacgctct gcctcacgct
     3841 cggcggcacg ctcggcagcg cggcgttcgg cggcctgctg ggcagcgagt tcggcctgcc
     3901 tcctggcctc ttccctggct tcctgcctgg cggccctctg ctgggccagc ctggcggccc
     3961 gctc
//
LOCUS       DBNGVD010000442         1815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2109644, whole genome shotgun sequence.
ACCESSION   DBNGVD010000442 DBNGVD010000000
VERSION     DBNGVD010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2109644"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16CM_11960"
     CDS             complement(<1..650)
                     /locus_tag="AC16CM_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3140593.1"
                     /translation="MREDDGYIDTHLREPLVEENTFWRMMPEDVKPPSFDEAWEVLPR
                     PFWEGHEDVLACYRGAWELAFGNLRRPEPGSGFVASFIDTAFNDCLFMWDSAFILLFA
                     RYGRRAFDFQRTLDNLYASQHPDGFICREISTLDGRDRFERFDATSTGPNVLPWTEWE
                     HYRDTADRERLARVFPVLLAYHGWLRRHRTWRDGTYWTSGLGSGMDNQPRLPQRPAT"
     gene            complement(710..1465)
                     /locus_tag="AC16CM_11965"
     CDS             complement(710..1465)
                     /locus_tag="AC16CM_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3140594.1"
                     /translation="MRRDQISLQLYTVREETARDMPGTLRKVSDIGYPAVEFAGYGGL
                     ATEDLRSILDDLGLRTSGAHVPLDSWEESPEAVLTDMETLGCAHAVLPIVPPERRRDE
                     ASVARLAEDLNRWGEQCLEHGVTFSYHNHDFEFAPLDNTTMWDVLVSETDPGLVGLEL
                     DLYWVKYAGSDPETVLRDVADRVSLVHLKDMAPDDSRSDLPVGEGTLPWVALLETADA
                     AGVQWFIAEQDKPRDALEDVRISLEHLHELAST"
     gene            complement(1490..>1815)
                     /locus_tag="AC16CM_11970"
     CDS             complement(1490..>1815)
                     /locus_tag="AC16CM_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3140595.1"
                     /translation="RESSDDFGVTLYGTEGGAEMLVRNYGTSNTVRIYTDVAGVPAVV
                     MPEIEPREGHYAVVKRFVETIHSGDWKGQHGEDGLDRARIIDACYASALQNREVSMEE
                     FRADR"
BASE COUNT          301 a          576 c          588 g          350 t
ORIGIN      
        1 gttgcgggac gctgcgggag cctgggctgg ttgtccatcc cggagccgag gcccgaggtc
       61 cagtaggtgc catctcgcca ggtgcggtga cgcctgagcc acccgtgata ggcgagcagg
      121 acggggaaga cgcgggcgag gcgttcgcgg tccgccgtgt cgcggtagtg ttcccactcg
      181 gtccacggaa gcacgttcgg gcccgtgctc gtcgcgtcga agcgctcgaa gcgatccctg
      241 ccgtcgaggg tagagatctc ccggcagatg aacccgtcgg ggtgctggct ggcgtagagg
      301 ttgtcgaggg tgcgctggaa gtcgaaggcg cgcctcccgt agcgcgcgaa cagcaggatg
      361 aacgccgaat cccacatgaa caggcagtcg ttgaaggccg tgtcgatgaa gctcgccacg
      421 aagccgctcc ccggctcggg gcgacgcagg ttgccgaacg ccagctccca ggctccacga
      481 tagcaggcca gaacatcctc gtgcccctcc cagaaaggcc ggggcaaaac ctcccacgcc
      541 tcatcgaaag agggcggctt gacgtcttcc ggcatcatcc gccagaacgt gttctcttct
      601 accagaggtt ctctcagatg ggtgtcgatg tagccatcgt cctcccgcaa ccgttcaacc
      661 tccccccgag cacgcctcgg tccgcggctc tgctattagc tcttgtctat caggtgctgg
      721 caagttcgtg caggtgttcg aggctgatcc tgacatcttc cagggcgtcc cgtggcttgt
      781 cttgctctgc tatgaaccat tgcacgcctg cggcgtcggc ggtctccagt agcgcgaccc
      841 atggcagcgt tccctcccct accggaaggt cggagcgcga atcgtcaggg gccatgtcct
      901 tgagatggac cagggagacg cggtctgcca catcccgcag gacggtttca gggtcgctgc
      961 cggcgtactt gacccagtag aggtcaagct ccagccctac aagtccgggg tcggtctcac
     1021 tgaccagcac gtcccacatg gtcgtgttgt cgagcggtgc gaactcgaaa tcgtggttgt
     1081 ggtaggagaa ggtcacgcca tgctcgaggc actgctctcc ccagcggttg aggtcttcgg
     1141 ccaacctggc tacagaggcc tcgtccctac ggcgctccgg tgggacgata ggcaacacgg
     1201 cgtgggcgca gcccagggtt tccatgtcgg tgaggaccgc ctccgggctc tcctcccagg
     1261 agtcgagtgg gacgtgggct cccgaggtcc ggaggccgag atcgtcgagt atggatctga
     1321 ggtcctcagt ggcgaggccc ccgtaacccg cgaactccac agcaggatag ccgatgtcgg
     1381 agaccttgcg caacgtgccc ggcatgtcgc gggcggtctc ttcgcggacg gtgtagagct
     1441 ggagtgagat ctgatcccgc ctcatgctat cctcccaatg gtttctttct catctgtccg
     1501 cacggaactc ttccatcgag acctcacgat tctgcagggc ggacgcgtag catgcatcga
     1561 tgatgcgcgc ccggtcgagg ccgtcttcgc cgtgctggcc cttccagtcg ccgctgtgga
     1621 tggtctcgac gaagcgcttg acgacggcgt agtggccttc acggggctct atctcgggca
     1681 ttaccacagc tggaacgccg gccacatcgg tgtagatgcg taccgtgttg ctggtgccgt
     1741 agttcctgac cagcatctcg gcgccgcctt ccgtaccgta gagggtgacg ccgaagtcat
     1801 cgctcgactc gcggt
//
LOCUS       DBNGVD010000443         6029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3203107, whole genome shotgun sequence.
ACCESSION   DBNGVD010000443 DBNGVD010000000
VERSION     DBNGVD010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3203107"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="AC16CM_11975"
     CDS             <1..1318
                     /locus_tag="AC16CM_11975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3140596.1"
                     /translation="LAVLGTLAVFGSAAVLLATGFSGTFFGGGYVVDGFALYFKLIVA
                     GSAFFAVLAASRWSGDTGDAPEYLMLILSVVLGSMLLVSMRDLFGIFLAIELATIPSY
                     AMVAFDRSRRESAEGGMKYLITGVIASSVLLYGLVLIYGVSGSAMLSDVADTFTGSLS
                     PVAVLGLVLMFSGFAFKLSAAPFHFWTPDAYQGAPTSAAAFLSVGPKAATFAVLLRIL
                     LEGMPGAAATWTAVAAFLAIITMFVGNLAALRQRNVRRMLAYSSVAHSGYILAAFAAL
                     QGIGVAFAVQAVLIYSAAYAIMNMGAFLVIDLVGEDTKNFNGLIRTNPGVAVSMAVFM
                     ASLVGIPPLAGFFGKLWIIIAGAQSGSVLVYVVIGALVVNTVISVPYYFGIIRNMILE
                     EPTTETVRSKGAGAVRFSVYALAVLATVFGLLIIPLSVLVGASGLT"
     gene            1384..2487
                     /locus_tag="AC16CM_11980"
     CDS             1384..2487
                     /locus_tag="AC16CM_11980"
                     /EC_number="2.3.1.-"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3140597.1"
                     /translation="MRAVVLVGGQGSRLRPITFDVPKALVPLRNRPFMGYMLDFLRRG
                     GVSGAVLSLGYLPDPIQAYLGGKQDLGGFSVDYAVENYALGTAGGIKNAEKYLDDETL
                     VVVNGDVLTGMDLSTAIEVHRSTDALATITLTSVEDPTAYGLVEVDHEMLVRRFLEKP
                     GADEVTTNLVNAGIYVLEPEVLDMIPSGREVSIEREIFPELQASGKMRAYITSSYWRD
                     IGTPRSYLAASHDVLSGAVGLHEDFDYLHIDPSVKSGKNVRLLPPVSIDEGCRISELA
                     TIGGRTSLGRDCVVGEGAVVEGSVVLDGAEIEAGAVVRGSIVGPNARVGNKSIVRGLS
                     VLGAGCVVGEGNVLDQGIRINPGVVVSPRGIRF"
     gene            2632..3282
                     /locus_tag="AC16CM_11985"
     CDS             2632..3282
                     /locus_tag="AC16CM_11985"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3140598.1"
                     /translation="MFYPELCVGCGRRSCDVLCRGCFEALPRVGTPACERCGLPTAFE
                     TFVCEACKNVDFGFESARAPLRYEGVGKEVVHALKYRGYRKVVAGLATPLMLQVLGRG
                     RFDAVVPVPLHRSRQRKRGFNQAGLLARGVAAEINAPLSDTLKVVRSTRDQVELSAAQ
                     RRANVAGAYRASTPLEGRILVIDDVFTTGATTSACASTLLQAGASEVHALCLCRTC"
     gene            3463..4035
                     /gene="hpf"
                     /locus_tag="AC16CM_11990"
     CDS             3463..4035
                     /gene="hpf"
                     /locus_tag="AC16CM_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3140599.1"
                     /translation="MDVLVKGHNIPVTEALERYAKEKVERVARFFDEGRSDSRAEVEL
                     IHERNRAVSEPEVAEATLFINGSVLKASEASEDMYASIDRMSDKLERQVKRYRGRQID
                     RWQGQLKNASGVPGGPERPFVVEEEEEIETRIVRTKQFQMKPMGEEEAVLQLELLDHD
                     FYVFTSADTGDINVVYRRRDGNYGLIEPAR"
     gene            4069..>6029
                     /gene="secA"
                     /locus_tag="AC16CM_11995"
     CDS             4069..>6029
                     /gene="secA"
                     /locus_tag="AC16CM_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3140600.1"
                     /translation="MANLLTKILRMGEGRKVKALQQRVEAVTALEPEVEKLQDSELKA
                     KTEEFRERIAGGETIDDLLPEAFSVVREVSRRTLGMRPFDVQVMGGIVLHEGKIAEMK
                     TGEGKTLAATMPVYLNALEGKGAHVVTVNDYLARRDASWMGEIYNFLGLEVGLVQESM
                     SFDERKVAYHSDITYGTNAQFGFDYLRDNIATSTEQLVQRDLNFAIVDEVDSILVDEA
                     RTPLIISGMPESAADVYYRFAAIVPRLVAGEDYEVDEKKQQVAPTESGVEKVENALGI
                     ENLYDDVNSNLVNHLNQALRAQTLYHKDDEYIVTDGQVFIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVKILEENQTVATITIQNFFRMYDKLSGMTGTAATEADEFMHTYKMEVVS
                     IPTHRDMVRDDKDDLVYKTEKAKFQAVADDIAERNKLGQPILVGTVSVDVSERLSALL
                     KRRGIAHNVLNAKQHEREADVIAEAGDRGAVTIATNMAGRGTDIKLGEGVPEVGGLYV
                     LGTERHEARRIDNQLRGRSGRQGDPGESRFYLSFEDELLRLFGGQRMQGVIDRIGLEE
                     DVPIEAGVISNSVRRAQEQVESRNFQIRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTMSYVEDALTGVVTEYVSDDVYPEDWDL"
BASE COUNT         1117 a         1852 c         1975 g         1085 t
ORIGIN      
        1 cctggccgtg ctaggtaccc ttgccgtgtt cgggagcgcg gccgtcctgc tcgccacggg
       61 cttctcgggg accttcttcg gtggcggcta cgtggtcgac ggtttcgcgc tgtacttcaa
      121 gcttatcgtc gccggctcgg cgttcttcgc ggtgctcgcg gcgtcgcgct ggtcggggga
      181 tacgggcgac gcgcccgagt acctcatgct catcctctcg gtcgtgctcg ggtcgatgct
      241 cctggtctcc atgcgggacc ttttcggcat cttcctcgcg atagagctcg cgacgattcc
      301 atcctacgct atggtggcgt tcgacaggag ccgccgtgag agcgccgagg gcggtatgaa
      361 gtacctcata accggcgtta tagcctcctc cgtgctcctc tacggcctcg tgctcatcta
      421 cggggtctcc gggtcggcga tgctctccga cgtggccgac acgttcacgg gcagcctctc
      481 gccggtcgcg gtccttggcc tggtgttgat gttctccggg ttcgccttca agctctcggc
      541 cgcgcccttc catttctgga cccctgatgc ctaccagggc gcacccacga gcgccgcagc
      601 cttcctctcg gtcgggccaa aggcggcgac cttcgcggtc ctcctgcgca tactcctcga
      661 gggcatgccg ggggcggcgg cgacctggac tgccgtcgcg gccttcctcg ctatcatcac
      721 catgttcgtg ggtaacctgg ccgcactcag gcagcgcaac gtgaggcgga tgctcgcgta
      781 cagctccgtt gcccactcgg gctacattct ggccgccttt gcggcgctgc agggaatcgg
      841 cgtcgctttc gccgtgcagg ccgtcctgat ctactcggcc gcctacgcca tcatgaacat
      901 gggcgctttc ctcgtgatcg atcttgtcgg ggaggacacc aagaacttca acgggctgat
      961 cagaaccaac cccggggtcg cggtgagcat ggcggtgttc atggcctcgc tcgtcggcat
     1021 tccgccgctc gccgggttct tcgggaagct ctggatcatt atcgccggcg cccagtccgg
     1081 gtcggtcctg gtctacgtcg tgatcggcgc gctggtcgtg aacaccgtga tctccgtccc
     1141 ctactacttc ggcatcataa ggaacatgat cctggaggaa ccgaccacgg agacggtgag
     1201 gtcgaagggg gccggcgccg tcaggttctc cgtgtacgcg ctggccgtac ttgccaccgt
     1261 gttcggccta ctcatcatcc ccctctccgt cctggttgga gcctcgggcc ttacgtaagc
     1321 gatacaagga tcgtttgttc cgtattggtc tgcgagccgc atctggtgta agcttgttcg
     1381 gcgatgcgag cggtagtact ggtaggagga cagggtagtc ggctgcggcc gatcaccttc
     1441 gatgtgccca aagcattggt acccttacgg aatcggccgt tcatgggtta catgcttgat
     1501 ttcctgcgcc gcggcggggt ttcgggggcc gtgctctctc tggggtacct gccagacccg
     1561 atccaggctt atctgggtgg caagcaggac ctgggcggct tctccgttga ttacgcggtc
     1621 gagaactacg ccctcggcac cgctggcggc atcaagaacg ccgaaaagta cctcgacgac
     1681 gagacgcttg tggtggtcaa cggagacgtg ctcaccggga tggacctctc cacggcgata
     1741 gaggttcaca ggtcgacaga cgccctggca accatcacgc tgaccagcgt ggaggacccg
     1801 acggcctacg gcctggtcga ggtggaccac gagatgctcg tgcgccgctt cctcgagaag
     1861 ccgggggcgg acgaggtgac gaccaacctc gtcaacgcgg gcatctacgt gctcgagcca
     1921 gaggtcctcg acatgatccc atcagggcgc gaggtctcca tcgagcgtga gatcttcccc
     1981 gagttgcagg cgagcggcaa gatgcgggcc tacatcacca gctcttactg gcgcgacata
     2041 ggcacgcccc gcagctacct ggccgcctcc cacgacgtgc tctcgggcgc tgtcggcctg
     2101 cacgaggact tcgattacct gcacatcgac ccttccgtga aatcaggcaa gaacgtcagg
     2161 cttctaccac ccgtctctat cgacgagggg tgccggatct ccgagctcgc cacgataggc
     2221 ggacgtacct cgctggggcg ggactgcgtt gtgggcgaag gggccgtggt ggagggcagc
     2281 gtggtacttg atggtgccga gatcgaggcc ggcgccgtgg tgcgaggctc catcgttggc
     2341 ccgaacgcga gggtgggcaa caaatcgatc gtgcggggtc tctcggtgct cggcgccggg
     2401 tgcgtggtcg gcgagggcaa cgtgctggac cagggtatac gcatcaaccc cggcgtggtg
     2461 gtctcgcccc gcggcatacg attctaggcg gttcgctggg cgaaccgcgc tgtcagcgtt
     2521 cagctgtcag ccttcagctt ttggcctccc gacccgacta attctacgac tacgccagcg
     2581 gccggtatag gagttcaatt gtacgaacct tacgtggccg cacttgccga catgttctat
     2641 ccggagcttt gtgtcgggtg tggtcgacgc tcttgcgacg tgctctgtcg cggctgcttc
     2701 gaggcccttc cgcgggtcgg cacccccgcg tgcgaacggt gcggtttgcc caccgctttc
     2761 gagacgttcg tgtgcgaggc ttgcaagaac gtggatttcg gcttcgagag cgccagggcg
     2821 ccgctccgct acgagggagt ggggaaggag gtcgtgcacg ctctcaagta ccgcgggtac
     2881 agaaaagtgg tagccgggct tgccacgcct ctgatgctcc aggtactcgg ccgtggccgc
     2941 ttcgatgccg tcgtgcccgt gccgctccac cgctcgcggc agagaaagag gggcttcaac
     3001 caggccggac tccttgcacg gggtgtcgcc gcagagataa acgcaccctt atcggataca
     3061 ctgaaagtcg tgcgcagcac aagggaccag gtcgagctat ctgcggccca gaggcgggcc
     3121 aacgtcgcgg gtgcctacag ggcaagcacg cccctcgaag gccggatact cgtgatcgac
     3181 gacgtcttca ccaccggcgc caccacgagc gcctgcgcct cgaccctgct ccaagccggc
     3241 gcaagtgaag tacacgcact atgcctgtgc aggacatgtt agttgtcagc cgtcagccat
     3301 cagctgtcag caagagcgtg cggaccgcga cccggggcat gccctcctat cccgttctcg
     3361 acgcaaagct gtggggaaga tacgagggtg caagcgcccg agtaccgagc cgaaccaaaa
     3421 gctgatggct gacagctaaa tccgaccgaa gggaggacca ttttggacgt actagtcaag
     3481 ggtcacaaca tacccgtgac cgaggccctc gagcggtacg cgaaggagaa ggtagagcgg
     3541 gtcgcccggt tcttcgacga ggggcgcagc gattcgcggg ccgaggtgga gttgatccac
     3601 gagcgaaacc gggcggtatc cgagcctgag gtggcagagg cgacgctgtt catcaacgga
     3661 tccgtgctca aggccagtga ggcctccgaa gatatgtacg cctccatcga caggatgtcg
     3721 gacaagctag agcggcaggt caagcgctac aggggacgcc agatcgaccg ctggcagggc
     3781 cagctgaaga acgcttccgg ggtgccgggc gggccggagc ggcctttcgt ggtggaggaa
     3841 gaggaggaga tcgagacccg catcgtgagg accaagcagt ttcagatgaa gcccatgggc
     3901 gaggaggagg cggtcctgca gttggagctt ctcgaccacg acttttacgt gttcaccagc
     3961 gccgataccg gtgatataaa tgtggtatac cggcgtaggg acgggaacta cgggctcata
     4021 gagcctgccc gctaatccgg ctagtccaca cagataaggt agcttgtttt ggcgaacttg
     4081 ttgacgaaga tattgaggat gggcgagggc cgaaaggtca aagccctgca gcagcgtgtc
     4141 gaggcggtaa ccgcgctcga gcccgaggtg gaaaagctcc aggactcgga actcaaggcc
     4201 aagacggagg agttcaggga gaggatcgcc ggcggcgaga ccatcgacga cctgctgccc
     4261 gaggccttct ctgtcgttcg tgaggtctcc aggcgcacgc tcgggatgag gcccttcgac
     4321 gttcaggtca tgggcggcat cgtgctgcac gagggcaaga tcgccgagat gaagacaggc
     4381 gagggcaaga cgctagccgc caccatgccc gtgtacctga acgccctgga gggcaagggc
     4441 gcccacgtcg tcaccgtcaa cgactacctg gcgaggcgcg acgcgtcctg gatgggggag
     4501 atctacaact ttctcggcct cgaggttggc ctggtccagg agtcgatgag cttcgacgag
     4561 cgaaaagtcg cctaccattc ggacatcacc tacggaacca acgcccagtt cggtttcgac
     4621 tacctgcgcg acaacatagc tacctccacc gaacagctcg tgcagcgcga cctcaacttc
     4681 gccatcgtgg acgaggtcga ctcaatcctg gtcgacgagg cgcgaacgcc cctgatcatc
     4741 tcgggcatgc cagagagcgc cgccgatgtc tactaccggt tcgccgccat cgtgccgcgc
     4801 ctggtggccg gcgaggacta cgaggtggac gagaagaagc agcaggtggc cccgacggag
     4861 agcggcgtgg agaaggtcga gaacgccctc ggtattgaga acctctacga cgatgtcaac
     4921 agcaacctcg tcaaccacct caaccaggcc ctcagggcac agacgctcta ccacaaggac
     4981 gacgagtaca tcgtcaccga cgggcaggtc tttatcgtgg acgagttcac ggggcgcgtg
     5041 ctggagggcc ggcgttactc cgagggtttg caccaggcca tcgaggccaa agagggtgtg
     5101 aagatcctgg aggagaacca gaccgtcgcc accatcacca tccagaactt cttccggatg
     5161 tacgacaagc tctccggcat gaccggcacg gccgccaccg aggccgacga gttcatgcac
     5221 acctacaaga tggaggtcgt ctccatcccg acccacaggg atatggtcag ggacgacaag
     5281 gatgacctgg tctacaagac ggagaaggcc aaattccagg cggtcgccga cgatatcgcc
     5341 gagcggaaca agctgggcca gccaatcctc gtgggcacgg tctcggtgga tgtctccgag
     5401 cggctctccg cgctgctcaa gcgtcgcggc atcgcgcaca acgtcctcaa cgccaagcag
     5461 cacgagcgcg aggctgacgt aatcgccgag gccggcgatc ggggggctgt caccatcgcg
     5521 accaacatgg cgggccgcgg cacggacatc aagctgggtg agggcgtccc tgaggtcggg
     5581 ggactctacg tgctcggcac ggagcgccac gaggcgaggc gcatagacaa ccagctcagg
     5641 gggcgttctg ggaggcaggg ggaccctggc gagtccaggt tctacctctc cttcgaggac
     5701 gagctcctgc ggctcttcgg cgggcagcgt atgcagggcg tcatcgaccg catcgggctc
     5761 gaggaggacg tgccgatcga ggctggggta atctccaact ccgtaaggcg cgcccaggaa
     5821 caggtcgaga gcaggaactt ccagatcagg aagcgcatcc tggagtacga cgacgtgctc
     5881 aacaagcagc gagaggtgat ctacgccata aggcgcgaca tcttgatggg cgaggacgtc
     5941 gacaccatga gctacgtgga ggacgcgctg acgggtgtcg tgaccgagta cgtctcagac
     6001 gacgtctacc ccgaagactg ggacctgga
//
LOCUS       DBNGVD010000444         3449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2838095, whole genome shotgun sequence.
ACCESSION   DBNGVD010000444 DBNGVD010000000
VERSION     DBNGVD010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2838095"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC16CM_12000"
     CDS             <1..677
                     /locus_tag="AC16CM_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR3140601.1"
                     /translation="PKLSARDLPVAVAKGSHGATTVAATARLAALAGIRLFATGGLGG
                     VHREARESWDVSADLAALAATPVGVVCSGVKSILDVPATLEYLETLGVPVVGFRTLRF
                     PGFYLADSGSPLGWSVDSEEEAARVIRNLPAVGLQDSGLVIANPLAEEEQLTPELHES
                     ALRAGLEELERRGVRGGDVTPFLLDRFAAETKGESLRVNRVIILRNARLAARIAVALS
                     GLDRPT"
     gene            681..1571
                     /locus_tag="AC16CM_12005"
     CDS             681..1571
                     /locus_tag="AC16CM_12005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3140602.1"
                     /translation="MENPRVVIVGDLLYDLFAKVDGELRLGIDTFTPIRATGGGSGAN
                     AAAWLATSGVETHLVGRVGDDAFGRFLEEELRGVGVRTHLARDPSTATGKVFVLVDGL
                     GERTMITDRGAAENLAVGDLPTSIFRGGHLHLPGYTFSGGSRRETAFEALRLARGAGM
                     TISVDPSAGPMLEDIGPDRFLAWTHGADLCFPNLEEGSLLAGAKDPDQITENLLDHYS
                     AVVLKLGAEGARYADVDGRRSRVPAATDRVQDTTGAGDSLCAGFLAAWLTGESPEEAL
                     RQGARVAARAVERVGGRPDG"
     gene            complement(1681..2034)
                     /locus_tag="AC16CM_12010"
     CDS             complement(1681..2034)
                     /locus_tag="AC16CM_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3140603.1"
                     /translation="MTQRQAARWVEAYLAAWRSNTPADIESLFTPDAVYFTAPHRTPW
                     RGHREIVRGWLDRADDQGEWDFRYEVLHVADDTAYVRGWTSYEECVVGNLWVVRFEEE
                     QRCRSFTEWWMVEDA"
     gene            complement(2042..2437)
                     /locus_tag="AC16CM_12015"
     CDS             complement(2042..2437)
                     /locus_tag="AC16CM_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140604.1"
                     /translation="MPRPPAFIDHIIIQVADLPRSLEFYRAALEPLGAEVTDIPGPPE
                     SWGPTVVLGPEDAEDLALIEGEPSSRLHLAFVAPNHEAVRKFHAAALASGGRDNGPPG
                     VREHYNPHYYAAFAIDPDGHNVEALFQGP"
     gene            2687..>3449
                     /locus_tag="AC16CM_12020"
     CDS             2687..>3449
                     /locus_tag="AC16CM_12020"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3140605.1"
                     /translation="MSFENTPHSGTKPGGVREALILTMVVLTLLVAACGAEPEDGASS
                     GSGELENSPGAAERSTTGRSEETTAGPVTETGSDEAAELARAEVGEREVTNMLPADGE
                     GPDPARQLPENPPEGIEVYPPTTNRTVEGPIDYSREPPTNGNHAPLWQNCGFYQEPIQ
                     NRHAVHSMDHGVVWITYHPDLPQEQLETLRPYGEENYVVVSPYPGQNAPVIATSWRVQ
                     LELDGADDPRLDRFVDEFRVSELAPLSGNRCTLGVG"
BASE COUNT          594 a         1158 c         1156 g          541 t
ORIGIN      
        1 tcccgaagct ctcggccagg gaccttcccg tggccgttgc gaagggaagc cacggcgcca
       61 cgaccgtggc ggcgaccgcc cgtctcgcgg cgcttgcggg gatacgcctc ttcgccaccg
      121 gcggcctcgg cggcgtgcac agggaggccc gtgagagctg ggacgtctcg gcggacctcg
      181 ccgcgctcgc ggcgacgccc gtcggcgtgg tctgctccgg ggtcaagtcc atcctcgacg
      241 tgcccgcaac cctggagtac ctggagaccc tcggcgttcc tgtcgtgggg ttccgtacgc
      301 tccgcttccc cggtttctac ctggccgact cgggcagccc gctcggctgg agcgtagata
      361 gcgaagagga ggcggcgcgg gtcatccgta acctccccgc cgtcggcctg caggactccg
      421 gcctcgtcat cgcaaacccg ctcgcggaag aagaacagct cacacccgag ctgcacgaga
      481 gcgccctccg cgcgggcctg gaagagctgg agaggcgggg cgttcgcgga ggggatgtga
      541 cgccctttct ccttgaccgc ttcgcggctg agaccaaggg cgagagcctg cgggtcaaca
      601 gggtcatcat cctccgcaac gcacgcctgg cggcgcgtat agccgtggcc ctctccgggc
      661 tagacaggcc aacctgaggc gtggagaacc cgcgggtcgt catcgtcggg gacctgctct
      721 acgacctgtt cgccaaggta gacggggagt tgaggttggg catcgatacc ttcaccccga
      781 tacgggccac gggtgggggg tctggggcca acgccgcggc ctggctggcc acctccggcg
      841 tcgagacgca cctcgttggc agggtcggcg atgacgcgtt tggccggttc ctggaagaag
      901 aactgcgcgg ggtgggcgta aggacgcacc tggcgcgcga cccttcaacc gccaccggca
      961 aggtgttcgt gctcgtcgac gggttggggg agcggaccat gatcaccgac cgtggcgccg
     1021 ctgagaacct cgccgtcggg gacctaccaa cctccatctt tcggggagga cacctgcacc
     1081 tccctggcta cacgttctcc ggcggcagcc gcagggagac cgctttcgag gccttacgcc
     1141 tcgcgcgcgg ggcgggcatg acaatctctg tcgacccctc tgcgggcccg atgctcgaag
     1201 acatcggccc cgaccgattc ctggcctgga cccacggcgc cgacctgtgt ttcccgaacc
     1261 tcgaagaagg ttcgctgctc gccggggcca aggatcccga ccagataaca gagaaccttc
     1321 tcgaccacta ttccgccgtc gtcctgaagc tcggcgccga gggggcccgc tacgccgacg
     1381 tcgatggccg gcgctcgcgt gtccccgcag ccaccgaccg ggtacaggac accacgggcg
     1441 cgggggactc cctgtgcgcc ggtttcctgg cggcatggct tacgggagag tcgcccgagg
     1501 aggccttgcg gcaaggtgcg cgggtcgcgg cccgcgccgt cgagcgtgtc ggcggtcgcc
     1561 cagacgggta acaggagacc gctctcccga tctccggttg gcggggacac cggagctcgc
     1621 agacgtccgg tggttcttcg ttgctcgaac ggatcgcgta gcgcgaacgc gacgcggcga
     1681 ctacgcgtcc tccaccatcc accactcggt gaagctccgg cagcgctgct cttcctcgaa
     1741 ccgcaccacc cacaggttgc cgaccacgca ttcctcgtag ctcgtccacc cgcgcacgta
     1801 ggcggtgtca tccgccacgt gcagcacctc gtagcgaaag tcccactcgc cctgatcgtc
     1861 ggcgcggtcg agccaccccc ggacgatctc ccgatgccct cgccacggcg tacggtgcgg
     1921 cgcggtgaag tagaccgcgt caggcgtgaa gagcgactct atgtcagcgg gtgtgttgct
     1981 cctccacgcg gcgaggtagg cttcgaccca ccgcgcggcc tgcctctgcg tcaccgctgc
     2041 tctagggacc ctggaagagc gcttcgacgt tatgcccgtc gggatcgatg gcgaaggcgg
     2101 cgtagtagtg agggttgtag tgctcgcgca cacccggcgg cccgttgtcg cgccccccgc
     2161 tggcgagggc ggcggcgtga aattttcgga ctgcctcgtg attcggggcg acaaaggcga
     2221 ggtgtagccg gctggacggc tctccctcga tgagcgccag gtcctcggcg tcttccggtc
     2281 ccagcaccac ggtgggcccc cacgactccg gcggtcctgg tatgtcggtc acctcggccc
     2341 ccagcggctc cagagccgcc cggtagaact ccagggagcg tggaagatcg gcgacctgga
     2401 tgattatgtg atcgataaag gcggggggac gaggcatgaa ctcatggtac tgctacagcg
     2461 ttcgaggcgt caagtgagag agcaaggtct acgcagcatt cgggggaggt gcgagtccag
     2521 aagcaagccc ggcagaagga gcacccggaa aagctcggtc tgccgacgcc gcggatgtcg
     2581 tgagggtatc gaaaaccgaa cggtattaca gcgaccgcgg caaggttcgt agtatcttcg
     2641 gattcgtaga tacccctgcg tagagtgaac aggaaggtag tccctcttga gcttcgagaa
     2701 cacaccacat tcggggacga agccgggtgg agtcagagaa gccctcatcc tcacgatggt
     2761 cgtgctcacg cttctcgtgg ccgcctgcgg ggcagaaccg gaagacggcg cttcttccgg
     2821 ttcgggcgaa ctggaaaact ctcccggagc ggcggagcgg agcaccacgg gccgatcgga
     2881 agagaccacc gcagggcctg taacggaaac cggctcagac gaggctgccg agctcgcgcg
     2941 ggccgaggtt ggcgagcgtg aggtcaccaa catgttgccc gctgacggcg aggggccgga
     3001 tccggccagg caactgcccg agaacccgcc ggaaggcata gaggtctacc cgccgaccac
     3061 caaccgtacc gtcgagggac ccatcgatta cagcagagag ccgcccacca acggtaatca
     3121 cgccccactg tggcagaact gcggcttcta ccaggaaccc atacagaaca ggcacgccgt
     3181 acacagcatg gaccacgggg tggtctggat cacctaccat cccgacctgc cgcaagagca
     3241 actcgaaacc cttcgccctt acggtgagga gaactacgtc gtggtcagcc cctacccggg
     3301 ccagaatgca ccggtaatcg ccacctcgtg gagggtccag ctagagctgg acggcgccga
     3361 tgacccacgc ctcgaccggt tcgtggatga gttcagggtc tccgagctgg cgcccctctc
     3421 cggaaaccgg tgcaccctcg gcgtgggca
//
LOCUS       DBNGVD010000445         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4114560, whole genome shotgun sequence.
ACCESSION   DBNGVD010000445 DBNGVD010000000
VERSION     DBNGVD010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4114560"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1866
                     /locus_tag="AC16CM_12025"
     CDS             106..1866
                     /locus_tag="AC16CM_12025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3140606.1"
                     /translation="MAMVGLGEEEVEETLEGAEASGLIQAARDGYAFDHALTQQSLYA
                     GLSPMRRKRLHRSAGEGLEGLGEKVRHNRAAEISRHFVEGSSPGRALPYALLAGDEAE
                     AVFAHGDAKHHYQRALEFADYVGDGPASSQALEKLGGVLAASIRYDEALVALERAADA
                     YRDGDDPEGAVRAEARIAQTHFRRGTPEEGIARLSAHLASVDRPGVPEGVRSNLGPPY
                     GALAQLYYAHGQYAGCRDAAQRGAKLSRAVGDVRLLAETEKMLGIGLLWTDTPDEGVK
                     ALKEAVHLAERSGAFDTLSTALLPLYLAHVVRGDLELSRECCERGIAIAKKTDDTGLL
                     AMHTTNLGMRHYYTGDWWEARVCLERAVGLAQSTELSYFSCLPPAYLGVLCKAQGAWE
                     NASRHFSDAAALAREAGSPEVLGYAEARLLELDVLQDRPAEAIARLGPEPDVSDMTWW
                     YDVLLLSVLAEAHAETGDVAKAEEVADLALTRARRTRNRLDGVEALRVRAKILSMLGR
                     PEEATAALEEALSWSRAMPYPYAEARILREHGMIHLRERELEEARKWLSAALCIFGQL
                     GAKKEAERTEQVLRTLDPAL"
     gene            2124..>3430
                     /locus_tag="AC16CM_12030"
     CDS             2124..>3430
                     /locus_tag="AC16CM_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3140607.1"
                     /translation="MTGVFWRRPETDAWVVLLAGAVIALFVVATVLSRTADADAGGDT
                     GSDSTRCHKLPREVPELVPRAAVQDGDCLEDLTTAGTQQTGHTDRNDWSGLNAAGTHN
                     PTGVRGLQVDGYFPDDSRTNTNNGWLHDSQFVIRFPNDWNGKLIITGAPGNREQYAND
                     FIIGDWVLARGYAFASTDKGNTGIEFYDDGSAPGGSVLEWHRRVEQLTRNTRAAAQKY
                     YGRSPERTYITGISNGGYLTRYALENTPGLYDGGVDWEGTLFRAGGPNLLTYLPVALR
                     NYPEYEATGDQQAHDNMIRAGFQPGSEFLWDEHYTTYWDLTQRIYREEFDPGYDGALK
                     AGIPFCQPGTPNCDADYVYRNRPQKVRDAVRKVSLTGDIGKPLLTLHGTLDTLLPIRT
                     DSNVYSRLVRDRGHGDIYRYYKIGKGNHVDSYYDDNKRKLRPML"
BASE COUNT          654 a         1076 c         1169 g          531 t
ORIGIN      
        1 gttagcgagg cgatctccga aagggtctcc cgcctgcaac cacaaacgca ataagtcctc
       61 ggagaggcga gcgtcctggg acaggtgttt ggcttcgagg agctgatggc gatggtaggt
      121 ctcggcgagg aggaggtgga ggaaacactg gaaggggccg aagcctccgg cctcatccag
      181 gcggcaagag acggctacgc gttcgaccac gcgctcaccc agcagtccct ctacgcgggg
      241 ctctccccga tgcgccggaa gaggctgcac cgttcggcgg gagaggggct ggaggggtta
      301 ggggagaagg tcaggcacaa cagagcggcg gagatctcgc gtcacttcgt ggaaggatca
      361 tcacccgggc gggcgctacc gtacgctttg ctggccggag acgaagcgga ggcggtattc
      421 gcgcacggag atgcgaagca ccactaccag cgtgcgctcg agttcgcaga ctacgtgggt
      481 gatggccccg catcgtcgca agcgttggaa aagttgggcg gcgtgctggc tgccagcatc
      541 cgctacgacg aggctctcgt cgcgctcgaa cgggcggcgg acgcttaccg cgatggagac
      601 gacccggagg gagcggtgcg ggcagaagcc cggatcgcgc agacgcactt caggcgtgga
      661 accccggaag aaggcatagc tcgcctctcg gctcatctgg cgtcggtcga taggcccggc
      721 gttccggagg gtgtgcggag caacctgggc cccccctacg gagcgcttgc gcaactctat
      781 tacgcccacg gtcagtatgc cgggtgccgg gatgccgcac agcggggcgc gaagctctcc
      841 cgcgcagtag gggatgtccg cttgctggcc gaaacagaga agatgctcgg catcgggctc
      901 ctctggactg acacccccga cgagggggtg aaggccctga aggaagcggt ccacctggcg
      961 gagaggtccg gcgccttcga taccctgagc acagcgctct tgccgctcta cctggcgcac
     1021 gtggtacgcg gggatctgga actcagccgc gagtgctgcg agcgcggaat cgccattgca
     1081 aagaagacag acgacacggg tttgctcgcc atgcacacga cgaacctcgg catgcggcac
     1141 tactacacag gcgattggtg ggaggcccgg gtatgcctgg agcgcgccgt ggggctcgcg
     1201 cagagcaccg agctctctta cttctcctgc ctgccgcctg cttacctggg cgtcctttgc
     1261 aaagcacagg gggcgtggga gaacgcgtcc cggcacttct cggacgcggc cgccctggct
     1321 cgtgaagcgg gcagtccgga ggtgttgggc tacgcggagg cccggctgct ggagctggac
     1381 gtgctgcaag atcgccccgc cgaggcgata gcccgcctgg ggcccgaacc agacgtctcg
     1441 gacatgacgt ggtggtacga cgtcctgttg ctgtcggtcc tggcagaggc tcacgcggag
     1501 acgggggacg tcgccaaggc cgaagaggta gctgacctcg ccctgacccg cgcccggcgc
     1561 acacgcaacc ggctggacgg cgtggaggcg ttgcgtgtcc gcgcgaagat cctctcgatg
     1621 ttgggacgcc ccgaagaggc gaccgcagcg ctggaggaag ccctatcctg gtcgcgcgca
     1681 atgccctacc cttacgccga agcacgcatc ctccgcgagc acggcatgat ccacctgcgc
     1741 gaacgggagc tcgaggaggc acgaaaatgg ctctccgcgg cgctatgcat cttcggccag
     1801 ctgggggcga agaaggaggc cgagcggacc gagcaggtac tccggaccct ggatccggcc
     1861 ctctgaaacc ccggaagcct ctaccttctg tctttctcac cgcggatgcc agggatgttt
     1921 cgagcacgga ccgccgaaga ccaggggtta acgggccggc caggcaagta ccattcctct
     1981 tccgagaagc cggcgtgcta tcgggctgtg gcggtcttgc cggccttgga tgtggacgct
     2041 agaatcctca cgtcgaggta gggaggacca gcgaggatct tgccggtgcg gtttgctgcg
     2101 gcccgctggg aaaggagacc agcatgacag gcgtattctg gagacgccct gagacggacg
     2161 cgtgggtcgt gttgctcgcc ggggcggtca tcgcgctgtt cgtggttgcc acggtgctct
     2221 cgaggacggc ggatgccgat gcgggtgggg ataccggtag cgactccacg cgctgtcaca
     2281 agcttcccag ggaggtgccg gaactggtcc cgcgtgctgc ggtccaggac ggtgactgcc
     2341 tcgaggacct gacgaccgcc ggtacccagc agacgggaca cacggacagg aacgattggt
     2401 cgggcctcaa cgccgccgga acccacaacc ccaccggcgt ccgggggttg caggtcgatg
     2461 ggtacttccc cgacgactcg cgtaccaaca ccaacaacgg gtggctccac gactcgcagt
     2521 tcgtgatccg gttccccaac gactggaacg ggaagctcat cataaccggc gccccaggca
     2581 acagggagca gtacgccaac gacttcatca taggcgactg ggtgcttgcg aggggctacg
     2641 ctttcgcctc gaccgacaag gggaacacgg ggatagagtt ctacgacgac ggctccgcac
     2701 cgggcggctc cgttctcgag tggcaccgca gggtcgagca actcacgcgc aataccaggg
     2761 ccgcagcgca gaagtactac ggccgttcgc cggagcgcac ctacataacg ggcatctcca
     2821 acggcggcta cctcacccgt tacgccctgg agaacacgcc cggactctac gacggcgggg
     2881 tcgactggga gggcacgctc ttccgggcgg gcggacccaa cctgctcacc tacctgcccg
     2941 ttgcgttgag gaactatccc gagtatgagg ccaccgggga tcagcaggcc cacgacaata
     3001 tgatccgggc tggcttccag cccggctccg agtttctgtg ggacgagcac tacactacct
     3061 actgggacct cacgcagcgg atctaccgcg aggagttcga tccgggctac gacggggctc
     3121 tgaaggccgg tataccgttc tgccagccag gcacgcccaa ctgcgatgct gactacgtct
     3181 acaggaacag gccccagaag gtcagggacg cggtgagaaa ggtttccctg acgggcgaca
     3241 taggcaagcc gctgctcacg ttgcacggaa ccctcgatac gctgctcccc atccggacgg
     3301 actcgaacgt gtactccagg ctcgtcaggg acagggggca cggcgacatt taccgctact
     3361 acaagatagg gaaaggcaac cacgtagact cgtactacga cgacaacaag cgcaagctgc
     3421 gcccgatgct
//
LOCUS       DBNGVD010000446         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3932474, whole genome shotgun sequence.
ACCESSION   DBNGVD010000446 DBNGVD010000000
VERSION     DBNGVD010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3932474"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1411
                     /locus_tag="AC16CM_12035"
     CDS             101..1411
                     /locus_tag="AC16CM_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3140608.1"
                     /translation="MTLSRKDQALVEQAKAILSLNWTGEYTQPDPRLYPHQWSWDSAF
                     IALAYAHYDQARAEKELRHLFEAQWSNGLLPQIVFNPHFRDYFPGPSFWNADESPHAP
                     RDVETSGVVMPPVHASAVLAVYKGGGTKKFLEYAFHKLEAWHGYLYRERDPGEEGLVY
                     IRHPWESGMDNSPMWGPILLRLHLRAEQIPSYKRADTHVVSAQDRPVDAVYDRFAYLV
                     KFFANRDYDEARIREDCPFLVQDVLFNSLLCRAEHDMAEMARILGKDPAPFEQRSQKT
                     AQAIEEKLWDEERGVYFDYDLVSESPIEVFFAANFSPLFAGIPDEKRARRLVDALEND
                     GFCLSDESITPVPSYDMHGFGFSPVTYWRGPVWLNINWLLMHGLEDYGYREHADRLRK
                     SIVELCREEGFHEYFNPITGEGHGSDLFSWSAALLIDVLAKDKG"
     gene            1422..2189
                     /locus_tag="AC16CM_12040"
     CDS             1422..2189
                     /locus_tag="AC16CM_12040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3140609.1"
                     /translation="MRLENKVAVVTGSSSGIGASIAQAFAAEGAAVVVNYSRHEDPAQ
                     KVLAQIEGAGGRGIVVGADVSDPKEVEAMVQQAVTAFGRLDIMVNNAGMEHKMPFLET
                     PFEVWQETIAVNLTGAWLGCQAAAKQMAAQGDPGRIINVSSVHEDLAMPTNSPYCATK
                     GGVRMLMRTLAVELAPHQITVNNIAPGAIDTPMDAPLKQNPDLMTELLSEIPLGRMGK
                     PEDVANMAIFLASEDSSYVTGSTFFVDGGMIRHAGTL"
BASE COUNT          470 a          814 c          757 g          411 t
ORIGIN      
        1 gggaccgggt cagcagccgg cccgggcaga tcctgggtat cgtcgcggtg gtcctcagcg
       61 tcgtatccat cgtcctgagc ggcctcgcgg ggccgccgca gtgaccctgt ccaggaaaga
      121 ccaggcgctg gtcgagcagg ccaaggcgat cctttccctc aactggaccg gcgagtacac
      181 ccaaccggac ccgaggctct acccgcacca gtggtcctgg gactcggcct tcatcgccct
      241 cgcctacgcc cactacgatc aagcccgcgc cgagaaggaa ctcaggcacc tcttcgaggc
      301 ccagtggtca aacggcctcc tgccccagat cgtcttcaac ccgcacttcc gcgactactt
      361 ccccgggccc agcttctgga acgccgacga gagcccgcac gcccccaggg acgtggagac
      421 ctcgggcgtg gtgatgccgc ccgtccacgc gagcgccgtg ctggccgtct acaagggcgg
      481 gggaaccaaa aagttcctgg agtacgcttt ccacaagctc gaggcctggc acgggtacct
      541 ctaccgtgag agggacccgg gcgaagaggg gctggtctac atccgtcatc cctgggaatc
      601 ggggatggac aactccccga tgtggggccc catcctgctg cggttgcacc ttcgggctga
      661 gcagatcccc agttacaagc gggccgacac ccacgtcgtg agcgcgcagg atcgcccggt
      721 agacgccgtc tacgaccggt ttgcctacct ggtcaagttc ttcgccaaca gggattacga
      781 cgaggccagg atcagggaag actgtccctt cctggtccag gatgttctct tcaactcgct
      841 cctgtgccgg gcggaacacg acatggccga gatggcgcgc atccttggca aggacccggc
      901 gcctttcgag cagaggtcgc agaagacggc gcaggctatc gaggagaagc tctgggacga
      961 ggagcgcggc gtctacttcg actacgacct ggtctcagag agccctatag aggtgttttt
     1021 cgccgcgaat ttctcgcccc tgttcgccgg catcccggac gagaagaggg ccagacgcct
     1081 ggtcgatgcg ctggagaacg acgggttctg cctctctgac gaaagcataa cgccggtacc
     1141 gagctacgat atgcatggct tcgggttctc acccgtcacg tactggcggg ggccggtgtg
     1201 gctgaacatc aactggcttc taatgcacgg gctggaagat tacggctacc gtgagcacgc
     1261 cgaccgcctg cggaagagca tcgtcgagct ctgccgggag gaaggtttcc acgagtactt
     1321 caaccccatc accggcgagg gacacggctc cgacctcttc tcctggtcgg cggccctcct
     1381 catagacgtg ctggcgaaag ataaaggata ggagaccgcc catgcggctc gaaaacaagg
     1441 tggctgtggt taccgggtcg tcttctggta taggggcgtc catagcgcag gccttcgccg
     1501 ccgaaggagc ggccgtggtc gtcaactact ccaggcacga ggaccccgcc cagaaggttc
     1561 ttgcccagat agaaggggcc ggtggcaggg gcatcgtggt cggggcagac gtctccgacc
     1621 cgaaggaggt cgaggccatg gtccagcagg ccgttaccgc cttcggccgc ctcgatatca
     1681 tggtcaacaa cgccggcatg gagcacaaga tgcccttcct ggagacgcct ttcgaggtgt
     1741 ggcaggagac catcgccgtc aacctgacgg gggcctggct cggctgccag gccgccgcga
     1801 agcagatggc cgcccaggga gaccccggcc gcatcatcaa cgtctcctcg gtgcacgaag
     1861 acctcgccat gccgaccaac tccccctact gcgcgaccaa gggcggggta aggatgctga
     1921 tgcgcacgct ggccgtcgag ctcgcgccgc accagataac cgtaaacaac atagccccag
     1981 gtgccatcga caccccaatg gacgccccgc tgaagcagaa cccggatctc atgacggagc
     2041 ttctctcgga gatccccctc ggccgcatgg gcaaaccaga ggacgtggcg aacatggcca
     2101 tcttcctcgc ctcggaagac tcctcctacg taacgggcag caccttcttc gtggacggag
     2161 gcatgatccg ccacgcagga acgctctaga gcagaccccg aaacggatta ccgccctgcc
     2221 ggagcgaggt atcgggtaac ctccccgtag gtggtcgttg ctcacgacgg ggaacccaaa
     2281 ccgtccggcg agggtttttt cggagacgtc cgggtttgcc cctgaccgta atagaatcgg
     2341 ccccagggac cgccgcacct cacagccatt tacgcaagca tcatctctac agcggcggtc
     2401 cttctcgctg gcgcctttct cgatcctccc gcgttcgcag gcagggactc gc
//
LOCUS       DBNGVD010000447         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3022032, whole genome shotgun sequence.
ACCESSION   DBNGVD010000447 DBNGVD010000000
VERSION     DBNGVD010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3022032"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="AC16CM_12045"
     CDS             <1..556
                     /locus_tag="AC16CM_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop domain-containing protein"
                     /protein_id="HIR3140610.1"
                     /translation="LHRTLGISTVVVVGGVGDYLDVADRVILLEDYRPSEATPRAREV
                     TDRFPPRTPLTPREVSAPRRRALDPSHISLQRGKRQAARGKGLHTIELGRDRVDLSYL
                     EQLAEAGQTEAVARIVGEWVASGKVRDVGELLTQALSSVGEQGLDSLGGFGGHPGQMS
                     LPRAQELAAAINRIRTLGAGPGKQ"
     gene            592..1374
                     /locus_tag="AC16CM_12050"
     CDS             592..1374
                     /locus_tag="AC16CM_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3140611.1"
                     /translation="MTEIRNDWAEEDADLNDSDTPSWFRSKAVAVHLYTASGAVLALL
                     MLVAAFQGEAVKALWLMLLCLLIDSTDGLLARRFRVREALPFFDGALLDNIVDYMTYV
                     FVPVFLLWSEGYLPAGNAGIVLAALPLLASSYQFCRVDAKTEDHYFLGFPSYFNVLAF
                     YAIVFQPGQLLLAAVLVACSFLVFVPIRYVYPTRTVAFRKISLSLTTLWLASYAAILW
                     QMPDPDPRILAFSILYLVYYFGLSFYLSAKMLEAHRTQQREA"
     gene            1560..>1998
                     /locus_tag="AC16CM_12055"
     CDS             1560..>1998
                     /locus_tag="AC16CM_12055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140612.1"
                     /translation="MASSVRRPPVGEVLLRDVEASDLPFFFEHQLDPEAARMADFQSR
                     DRESFMAHWGRILENESAVQKTILVEGEVAGNIVSFVSSGEREVGYWIGRGFWGRGVA
                     TRALAEFLRLEERRPLHARVVRHNVASVKVLEKCGFRVTGEEDD"
BASE COUNT          335 a          670 c          620 g          373 t
ORIGIN      
        1 cctgcaccgt acccttggca tctcgacggt cgtggtagtc gggggagtag gcgactacct
       61 ggacgtggca gaccgcgtga tcctgctaga agactacaga ccttcggagg caacaccccg
      121 cgcccgcgag gttacggaca ggttcccacc ccgcaccccg ctaacgccca gggaggtaag
      181 cgcaccccgc aggcgcgccc tcgacccctc ccacataagc ctccagcgcg gtaaacgaca
      241 ggccgcgcgg ggcaagggcc ttcataccat cgagctcggc cgcgaccggg tggacctctc
      301 gtacctggag cagctcgccg aggccggaca gacggaggcg gtggcgcgta tcgtggggga
      361 gtgggtcgcc tcgggtaagg tccgtgacgt gggcgagctc ctcacgcaag cgctctcctc
      421 ggtcggggag cagggcctcg actcactcgg cggcttcggc gggcaccccg gacagatgag
      481 cctgccgcgg gcccaggaac tggcggcggc catcaaccgg attcgcacgc tgggggcagg
      541 ccccggcaaa cagtagcccg cttgctccgg ctggtatcat aaagatctgg tatgacggag
      601 atccgaaacg actgggcgga agaagacgcg gacttgaacg actcggatac cccatcctgg
      661 ttcaggtcca aagcagttgc cgtacacctg tacacggcga gcggtgccgt gctcgcgctc
      721 ctgatgctgg tggcagcctt ccagggcgag gcggtcaagg cgctctggct gatgctcctc
      781 tgcctcctga tcgatagcac ggacggcctc ctcgccaggc gcttccgggt gagggaggcg
      841 ctccccttct tcgacggggc gctcctcgac aacatcgtgg actacatgac ctatgtcttc
      901 gtccccgtgt tcctgctctg gagcgagggc tacctgcccg ccggcaacgc cggtatcgtg
      961 ctggccgcgc tgccgctgct agcctccagc taccagttct gccgggtcga cgccaagacc
     1021 gaagaccact acttcctggg cttcccgagc tacttcaacg tgcttgcctt ctacgccatc
     1081 gtgttccaac cgggacagct cctgctcgcc gcggtgctgg tcgcctgctc gttcctggtc
     1141 ttcgtcccga tccggtacgt ctaccccacg cgcacggttg ctttccgtaa gatctccctc
     1201 tcccttacca cactctggct cgcgagctac gccgccatcc tctggcagat gccggacccc
     1261 gacccccgca ttcttgcctt ctctatcctc tacctcgtct actatttcgg cctgagcttc
     1321 tacctctccg ccaaaatgct agaagcacac cgcacccaac agcgcgaagc gtagagagca
     1381 cgcttcggcc ctacgggcct ccgctcgtcg gcatgctcgg gctagcgccc tcgcttgtcc
     1441 gcaacgcctc ggcttcgcct cgctttcggc taatgggcct gcgagcctac ccttcggggc
     1501 actgaaacac gtcgcgggtc ccacttgttc ttggatgatg ggttcattaa catgccttca
     1561 tggccagtag cgtcagaaga cctccagtgg gagaagtgct gctgcgggat gtggaagcgt
     1621 cggacctccc ctttttcttc gagcaccagc tagacccgga ggcggcccgc atggccgatt
     1681 tccaatccag ggacagggaa tcgtttatgg cgcactgggg caggattctg gagaacgagt
     1741 ccgccgtcca gaagaccatc ctcgtcgagg gcgaggtggc cggtaacatc gtgagcttcg
     1801 tgagctctgg cgagcgggag gtgggctact ggatcggcag agggttctgg ggcaggggtg
     1861 tcgccaccag ggcgctggcc gagttcctgc gcctcgaaga gaggcgtccc ctccatgcca
     1921 gggtggtccg tcacaacgtc gcttcagtca aggttctcga aaaatgcggg ttcagggtca
     1981 cgggcgagga ggacgacg
//
LOCUS       DBNGVD010000448         9519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3750514, whole genome shotgun sequence.
ACCESSION   DBNGVD010000448 DBNGVD010000000
VERSION     DBNGVD010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3750514"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16CM_12060"
     CDS             <1..186
                     /locus_tag="AC16CM_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3140613.1"
                     /translation="LPHDQREVLEMAYMRGWSQREISRHTGVPLGTVKTRTRLALKKL
                     RRALEPLRKEPVDLDGV"
     gene            176..961
                     /locus_tag="AC16CM_12065"
     CDS             176..961
                     /locus_tag="AC16CM_12065"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3140614.1"
                     /translation="MECDGRIKDLFSDYHLGQLQPEEAEEVRRHLAEHASCREALEEV
                     AEVLDLMPFAVPASAPPPSLKARVLARASEPETTESEAPEPEDPKPDNAPQPVVPART
                     GGGRWRIFLPAAAGAILVFLTVFAWAAYLGSEQENVRLRAENQRLEKQGGSSLVVVGV
                     EGTGLAPEARGTAVLDPADGTVALDVYNLPATPEGHSYRAWLVAPDGDAYSLGTMKTD
                     DRGDGRMAGRLPKDPTPYETVQVTTEPIGARERAGPVYLESGL"
     gene            complement(1177..1851)
                     /locus_tag="AC16CM_12070"
     CDS             complement(1177..1851)
                     /locus_tag="AC16CM_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3140615.1"
                     /translation="MKHRELREEVARVAKQMISSGLVTGTSGNVSARTPEGDVLMTPS
                     GLDYEQMEPGDVVLVDVNGEMLGGSLEPSTETPMHTGIYRSRPEVGAVVHTHSIFATT
                     LACLGWPIPPVHYMLTTLAEDASIPLAPYTTYGTEQLAANAAEALGEKRNACLLQNHG
                     TITVAENPEAAFSRTVVLEEMAEVYYRARTAGEPIILTPEQIGDVAAKISDYGQTKPA
                     RTDDQS"
     gene            2175..3713
                     /locus_tag="AC16CM_12075"
     CDS             2175..3713
                     /locus_tag="AC16CM_12075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3140616.1"
                     /translation="MAAERYVIGIDCSTTATKAVVWDELGHSVAEGRATFPLDAPRPG
                     WYEQDAEEWWRSITRALREATSRVDAGRIQALGITHQRESFVCTDGEGNPLRQAILWL
                     DARSYREVDRYGSDRIHEITGKPPSVTPALYKLLWLRENEPELLERTSRILDVHAFLV
                     RRLTGEWRTSWPCADPLGLLDMRSFDWSDEILGEIGLGREQMVELSPPGSVVGELDKH
                     VAQETGLPPGLPVIGGAGDGQAAGLGANVTEAGKAYLNLGTAIVSGTYSDDYSWGREF
                     RTLAGPIPETYVPETLLRGGTYTIDWFVDNIGGIKPEDLGLDISAEQVLETAAGQVPP
                     GAEGLLLLPYLNAASTPYWDSQARGVLFGLRGTHNKAHVYRAVLEGLAFEQRVQTDAV
                     EGGLGQPVERFLAMGGGSRSPLFCQMISDITGRPVTACQEVETTCLGAAMLAAAALED
                     TGDIREVAATMSGEGASYEPDEQTSAFYDHLYNNVFKELYPRLSDLFPALAKAVEDQD
                     RSGS"
     gene            complement(3816..4550)
                     /locus_tag="AC16CM_12080"
     CDS             complement(3816..4550)
                     /locus_tag="AC16CM_12080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140617.1"
                     /translation="MNERLYDDIAGWWPVISPPAEYAEEAALYVDMLREAARRPVREV
                     LELGSGGGNNASHMKQAFAMTLVEPSDGMRENSRRLNPECEHLPGDMRYVRLGRTFDA
                     VFLHDAVAYMTTEDDLRSALETVAVHLAPGGVALVAPDVTTETFSETTDHGGGEDEEG
                     RRARYLEWMLPPEPGESTPTVHYVFLLRDVDGNVSAVHDVHHEGLFPRVTWLRLFREV
                     GLDAELVARTIEGREYDSFVAVRAAP"
     gene            complement(4564..5769)
                     /locus_tag="AC16CM_12085"
     CDS             complement(4564..5769)
                     /locus_tag="AC16CM_12085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR3140618.1"
                     /translation="MTGMKPAFKGKGDAYGEQVLALRRHLHRFPELSGEEHDTSEMVQ
                     RKLEEHDISFTTGYAGTGVLGVVEGERPGKTVALRADMDALPIQEENDHDFVSEVEGV
                     MHACGHDAHTAMLVGAGWLLRDVKEELPGRVLLVFQPAEEASPTGGAQRMMKEGVFGE
                     HRPDVIFAQHVSPELPVGRVGVRRGAITGASDRFKVTIEGEGGHASKPHQTIDAIVVA
                     NQVINSLQTVVSRDVDPLRSAVLTVGKIRGGIRYNAIAQRATLEGTIRTFSPDTKERV
                     KERFHTIVRGVAESMEASVRIRYLDGYPPTVNTPEWAERIRKTAQDLLGDGATLEIEP
                     SLGGEDFGRFLLEYPGAYFRLGTASPHTSDRRRLHDSRFDIDESALGPGTELFAQVAA
                     DTLYQLDGR"
     gene            5822..6556
                     /locus_tag="AC16CM_12090"
     CDS             5822..6556
                     /locus_tag="AC16CM_12090"
                     /inference="COORDINATES: protein motif:HMM:NF040370.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase-like domain-containing protein"
                     /protein_id="HIR3140619.1"
                     /translation="MPHIKPVALWAVPRSISTALERVFVERGDYKVFHEPFSVSYYYS
                     PERRSERFASTGTKQEYGYREILDLMLGFEEKPVYFKDMAYHMAGIMSEELVSRFTNT
                     FIIRNPAPVIASLSRFWPDFTLEETGYEQQHRLFGLAIENGEDPAVVDAADLIGDPQG
                     TVRAYCEKLGVPFMPESLSWKPGPVPEWEMWTEWHEEAQDSIGIKSQPLEDDSEVPAG
                     LEDVYDRCLPYYQELHARRLVPNFGG"
     gene            complement(6667..6933)
                     /locus_tag="AC16CM_12095"
     CDS             complement(6667..6933)
                     /locus_tag="AC16CM_12095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140620.1"
                     /translation="MNVLVPPLLRDEYADKESQEKIVADSDLDWIIVRLMIMTNGPWT
                     NDYRADVDLKSGRRPYVSRADVAAFLLNQLTNDTFVRKAPSIGY"
     gene            complement(6930..7331)
                     /locus_tag="AC16CM_12100"
     CDS             complement(6930..7331)
                     /locus_tag="AC16CM_12100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140621.1"
                     /translation="MRLAIFGPTGGTGRRLVERAIAEGHDVTAFARNPSKVTARHERL
                     RIVVGDAFGATSVREAVDGNEAVICVLGFRTPSNPLHPRRPGHPHGVYSAGAESIVAA
                     MKVHGLRRFVCQTAWGWGRAGKTRDLPERSS"
     gene            7542..8915
                     /locus_tag="AC16CM_12105"
     CDS             7542..8915
                     /locus_tag="AC16CM_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140622.1"
                     /translation="MICALFVSVFTNTMVNVAIPLIREQFGASEAQSGWVITGYALTF
                     AVGIPLYGRVSDLFSLRRTFAFGMGILAVGLAVCAIAPTLTLLVAGRAVQGVGARAIP
                     AVAFGSIAKLLAPGQRGTALGALSSSVGVGAAAGPVLGGLIAGAAGWQALFYVTFGLS
                     LVVLAASIYVLPDRISDEPTEDATLRGLDLPGGLLLGGSAGLLLFGVTQAETMGLSSI
                     LVWGCLLLAAFAGASFAWHIRTTPEPFISPRIFTSRAFLAAALVGFLAQFANIGSLFL
                     APLLLEGAGGLSPAAAGLVLAPGAIAVAILSPFAGMLSDRLGPRTVLLFGLTFMLVST
                     LVVSSFAVGAAPYILAGGLLLLGIGYAGANSPAANAASEAVPREAAGTGLGIYQLFFF
                     LGSGAGPAVLGAFLASRRGADGGAINPLYSLKATPFSDAFLLAVLALTLALLATTRLD
                     GKPEGDE"
     gene            complement(9233..>9519)
                     /locus_tag="AC16CM_12110"
     CDS             complement(9233..>9519)
                     /locus_tag="AC16CM_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3140623.1"
                     /translation="VQEVLTDPLHPYTRSLLAVTPEIGRRRAHEVLTGEPPDPAHIPP
                     GCRFHPRCPVLRSGEAGAEEPRCLGVVPTLQELAPGHRVACHVATRGADE"
BASE COUNT         1562 a         3096 c         3151 g         1710 t
ORIGIN      
        1 ctgccgcacg accagcgcga ggtgttggag atggcgtaca tgcgggggtg gtcccagcgg
       61 gagatctcgc gccacaccgg tgttccgctt ggcacggtga aaaccaggac caggctagcc
      121 ctcaagaaac tccgtcgcgc actcgaacct ctgaggaagg agcccgtgga tctggatgga
      181 gtgtgacggc cgcataaagg acctgttctc cgactaccac ctcggccaac tccagccgga
      241 ggaggccgag gaggtgcgtc ggcacctggc cgagcacgcc tcctgccgcg aagccctcga
      301 agaggtcgcc gaagtgctcg acctcatgcc cttcgccgtg ccggcgtcag ccccaccacc
      361 ttcgctgaag gcacgcgttc tcgcccgcgc ttcggagccc gagaccacgg aatccgaagc
      421 tccggagccc gaggacccga agcccgataa tgcaccccaa cccgtcgtgc ccgccaggac
      481 ggggggagga cgctggcgga tcttcctgcc ggccgctgcc ggggctattc tggtctttct
      541 cacggtcttc gcctgggcgg catacctggg gtcggagcag gagaacgtgc ggctacgggc
      601 cgagaaccag cggctcgaaa aacagggcgg gtcttctctg gtcgtggtag gggtcgaggg
      661 tactggactt gcgcccgagg cgcggggaac ggccgtgctc gacccggcgg atgggaccgt
      721 ggcgcttgac gtatacaacc tgcctgccac gccggagggc cactcgtaca gggcctggct
      781 agtggcgccc gacggcgacg cctactccct gggaaccatg aagacagacg acaggggcga
      841 cgggcgcatg gcaggccgcc tcccgaaaga cccgacgccc tatgagaccg tgcaggtcac
      901 caccgagccc atcggggcca gagagcgagc agggcccgtg tacctggagt cgggcctcta
      961 ggtagatctc gaggtaaggc atcaggcttc aggagaagta cctctggccc gatgcccttt
     1021 tctgcgacgt agcctgactt tttccagagt accccgaacc gattacctcg ggacaaccgg
     1081 ctgaaagcga ggcgaagccg aggcgttgcc gacaagcgag ggcgtaagcc cgagcatgct
     1141 gacaagcgga ggcgtagccg gagcgtgctg acgagactag ctctggtcgt ccgtcctggc
     1201 tggtttcgtc tggccgtagt cggagatctt ggccgccaca tcgccgatct gctcgggggt
     1261 aaggattatg ggttctccgg cggtcctggc ccggtagtag acttcggcca tctcctccag
     1321 cacgacagtt ctggagaacg ccgcctccgg gttttccgcg acggtgatgg tgccatggtt
     1381 ctgaagcagg cacgcgttcc gcttctcgcc gagggcctcg gcggcgttgg cggctagctg
     1441 ctcggtaccg taggtggtgt aaggggcgag cgggatgctc gcgtcttccg ccagcgtggt
     1501 gagcatgtag tgaaccgggg ggatgggcca tcccaggcat gcgagcgtgg tcgcgaagat
     1561 ggagtgggta tgtaccacgg cccccacctc gggacgggag cggtagatcc cggtgtgcat
     1621 cggcgtctcg gtggagggct ctagagatcc ccccagcatc tccccgttca cgtcgacgag
     1681 cacgacatcg cccggctcca tctgctcgta gtcgaggccg ctaggggtca tgagcacgtc
     1741 accttccggg gtgcgggcac tcacgtttcc cgaggtcccc gtcacgagac cggaggagat
     1801 catctgtttg gcaacgcgcg ccacctcttc gcgaagctcg cgatgtttca tgaaagactc
     1861 ctatctgtga gcgggatccc gtgccctatg cctttcgccg ccctggtgcg tatcgggacc
     1921 tcgccggacc cgggctgcgg caccccatcc gtcgttgcgt ctacgggctc gttcaacatt
     1981 gctgccctca aagagagcca cctccccgtt gattgctcgt accagcatta ccgccggacc
     2041 agtttagcaa tcgtggttcg acgtgcgcag cgtgcgccgc ccggcacggg accggggggt
     2101 gctatagtgc gtcggggaac taacgaggca cgtgaaaggg gtctgtgtga ggacgacggg
     2161 gggtgctgcc tagcatggct gccgagcggt acgttatcgg gatcgactgc tcgaccacgg
     2221 ccaccaaggc cgtggtctgg gacgagttgg gccattccgt agcggaggga agggcgacct
     2281 ttccgctgga tgcgccccgg cccggctggt acgagcagga cgccgaggaa tggtggagat
     2341 ccatcaccag ggctctgaga gaggccacct cgcgggtgga cgcgggccga atccaggccc
     2401 tcggcataac gcaccagcgg gagagcttcg tgtgcacgga cggggagggc aacccactga
     2461 ggcaggccat cctctggctg gacgccagat cctacaggga ggtggacagg tacggctccg
     2521 accgtatcca tgagatcacg ggcaagccac cgagcgtgac ccctgccctc tacaagcttc
     2581 tctggctgcg cgagaacgaa ccggagctgc tggagcgcac ctccaggata ctcgacgtgc
     2641 acgccttcct cgtacgccgg ttgaccgggg aatggcgcac gagctggccg tgcgcggacc
     2701 cgcttggctt gcttgacatg cggagcttcg actggtcgga cgagatcctc ggggagattg
     2761 gcctcgggag ggagcagatg gtggagctgt ccccccccgg ctccgtggtt ggcgagctcg
     2821 acaagcacgt ggcacaggag acggggctcc ctccggggct tccggtcatc ggtggcgccg
     2881 gggacgggca ggccgccggt ctaggggcaa acgtgaccga ggccgggaag gcctacctca
     2941 acctgggtac ggccatcgtg tcaggcacct acagcgacga ctactcctgg ggccgtgagt
     3001 tccgaaccct cgcgggcccc atcccggaga cctacgtccc cgagaccctg ctgcgcggcg
     3061 gcacctacac catcgactgg ttcgtggaca acattggcgg tatcaaaccc gaggatctgg
     3121 ggttggacat cagcgcggag caggtcctgg agacggccgc cgggcaggtc ccaccggggg
     3181 ctgaggggct actccttctg ccttacctga acgcggcttc cacaccttac tgggactcgc
     3241 aggcgagggg ggtcctgttc ggcctgcggg gcacccacaa caaggcccac gtctaccggg
     3301 ccgtgctcga agggctggcc ttcgagcaac gcgtacagac cgatgccgtg gaaggcgggc
     3361 tcggccagcc ggtcgagagg ttcctggcca tgggcggcgg gtcacgcagc ccgctgttct
     3421 gccagatgat ctccgacatc acgggaaggc cggtgacggc gtgccaggag gtagagacca
     3481 cctgcctggg cgccgcgatg ctggccgccg ccgcgctgga agacaccggc gatattcgcg
     3541 aggtggccgc gaccatgtca ggcgagggcg caagctacga gccggacgag cagacgtccg
     3601 cgttctacga ccacctgtac aacaacgttt tcaaggagct ctacccccgc ctctcggacc
     3661 tcttcccagc tctggccaag gccgtcgagg atcaggacag gagcggatcg tgatggaacc
     3721 atgcccacat gaaggcaacc acctacgata gatagctgta gccgtcagct ttcagctgtc
     3781 agctacagga gctctcaccg ggaggctgtt gcgtactacg gtgcggcacg caccgcgacg
     3841 aacgagtcgt actcccggcc ttcgatggtg cgggcgacga gctctgcatc gagcccgacc
     3901 tcccggaaga gcctgagcca cgtcacgcga gggaacaggc cctcgtggtg cacgtcgtgg
     3961 accgcgctca cgtttccatc tacgtcgcgc agcaagaaca cgtagtgcac ggtgggggtg
     4021 gattcgccgg gctccggcgg tagcatccac tccaggtacc gggcccgtct tccctcctcg
     4081 tcctccccgc cgccgtggtc ggtggtctcc gaaaacgtct ccgtggtgac gtccggcgcg
     4141 accagcgcca cgccgccggg cgcgaggtgg acggccacgg tctcgagtgc cgaccgcagg
     4201 tcatcctcgg tggtcatgta cgcgacggcg tcgtggagga agaccgcgtc gaaggtgcgt
     4261 cccaggcgta cgtagcgcat gtcgccgggg agatgctcgc actcggggtt gagcctgcgg
     4321 ctgttctcgc gcatcccgtc cgagggttcg acgagggtca tggcaaaggc ctgcttcata
     4381 tgggaggcgt tgttaccgcc tccgctgccg agctcgagca cctcgcgcac cgggcggcgg
     4441 gcagcctcgc ggagcatatc cacgtaaagc gccgcctcct cggcgtactc ggcgggcggg
     4501 gagatgactg gccaccagcc ggcgatgtcg tcgtagaggc gctcgttcac gcacagcccc
     4561 tttctaccgg ccgtcgagtt ggtacagggt gtctgcagcc acctgggcga agagttccgt
     4621 ccccggcccc agcgctgact cgtcgatgtc gaagcgtgag tcgtgcaggc gtctcctatc
     4681 gctggtgtgc ggagaggcgg tcccgagcct gaagtaggcc ccggggtatt ccagcaggaa
     4741 gcgcccgaaa tcctctcccc cgagcgaggg ctctatctcg agagtcgcgc cgtcacccag
     4801 gagatcctgc gccgtcttgc ggatgcgctc ggcccactcc ggagtgttca ccgtcggcgg
     4861 gtagccgtcc aggtacctga tgcgcacaga ggcctccatc gactcggcca cgccgcgcac
     4921 tatggtgtgg aagcgttctt tcaccctctc tttggtatcg ggagagaacg tgcggatggt
     4981 accctccagc gtcgccctct gggcgatggc gttgtagcgg ataccacccc ggatcttgcc
     5041 cacggtcaga accgcggagc gcagggggtc cacgtccctg ctcacgaccg tctgcaaact
     5101 gttgatgacc tggttggcca ccacgatggc gtcgatggtc tggtggggtt tgctggcgtg
     5161 tccgccctcc ccctcgatgg tcaccttgaa acggtcggag gcacccgtga tcgcaccccg
     5221 ccgcacgcca acccgtccta cgggcaactc gggagaaacg tgctgagcaa agatgacatc
     5281 agggcgatgc tccccgaaca cgccctcttt catcatgcgc tgtgctccgc ctgtgggcga
     5341 ggcctcctcg gccggctgga acacgagcag cacgcggccc ggtagctctt ccttgacgtc
     5401 gcggagcaac catccagccc ccaccagcat tgccgtgtgg gcatcgtgcc cgcaggcgtg
     5461 catcacgcct tccacttctg agacgaagtc gtggtcgttc tcttcctgga tgggcaaggc
     5521 gtccatgtcc gcccgcaggg ccacggtctt gcccggacgt tccccctcca cgacgccgag
     5581 cacgccggtc cctgcgtagc ccgtggtgaa ggagatatcg tgctcctcca gcttccgttg
     5641 caccatctcg gaggtgtcgt gttcctcccc gctgagctcg gggaagcggt ggaggtggcg
     5701 ccgcagggcc aggacctgct ctccgtaagc gtcgcccttg cctttgaacg ccggcttcat
     5761 ccccgtcatc ctactgtagt agtatcgacc gtgaccgatt gtataggata aagggagcgc
     5821 gttgccgcat atcaagcccg tcgcactctg ggccgtgccc cggtccatct cgaccgccct
     5881 ggagcgggtg ttcgtcgaac gcggagacta caaggtcttt cacgagccct tctcggtctc
     5941 ctactactac tctccggagc gccgcagcga acggttcgcc tcaaccggaa ctaagcagga
     6001 atacgggtac agggagatac tggatctgat gctcggcttc gaggagaagc cggtctattt
     6061 caaggacatg gcctaccaca tggcgggcat catgagcgag gaactcgtct cgcggtttac
     6121 gaacacgttt atcatcagga atcccgcgcc ggtgatcgcg tcgctaagca ggttctggcc
     6181 cgacttcacc ctggaggaga cgggttacga gcaacagcac aggctgtttg ggctcgccat
     6241 agagaacggg gaagatcctg cggtggtgga cgccgccgac ctgatcgggg acccccaggg
     6301 cacggtccgg gcctactgcg agaagctcgg cgtgccgttc atgcccgagt ccctatcctg
     6361 gaaaccgggc cccgtgccgg aatgggagat gtggaccgaa tggcacgagg aggcccagga
     6421 tagcataggc ataaagagcc agccgctgga agatgactcg gaggtgccgg caggcctgga
     6481 agatgtctac gaccgctgcc tgccctacta ccaggagctc cacgccagac ggctggtacc
     6541 gaacttcggt ggatgatcac tgcgaccgtg cgttgggagc agggaaatag ggagtaggga
     6601 atggcgaaag gctttttccc ctactccctg tcacatcgag tagcgatgta ttcacccgtt
     6661 tacgcgtcag tagccgatgg acggcgcctt tcgcacgaag gtgtcgttgg tgagttgatt
     6721 tagcaagaag gcggccacgt ccgcacgaga gacgtagggg cgccggccgg atttcaggtc
     6781 cacgtccgcg cggtagtcgt tggtccaggg tccgttggtc atgatcatga ggcgcacgat
     6841 gatccaatcg aggtcgctgt ctgccacgat cttctcctgc gattctttat ccgcgtactc
     6901 atccctgagc aggggaggca ccagcacgtt catgaagaac gctccggcaa atcccgggtt
     6961 ttgcctgctc tcccccaccc ccaggccgtc tggcacacga agcgccgcaa accgtgcact
     7021 ttcatcgccg cgacgatgct ctctgcacca gctgaataca ccccgtgcgg gtgtccgggc
     7081 ctacgggggt ggagcgggtt gctgggggtc cgaaagccca ggacgcagat cacggcctcg
     7141 ttgccgtcca ccgcctcccg caccgaggtt gcaccgaaag cgtcccccac cacgatacgt
     7201 agccgctcgt ggcgtgcggt tactttggag gggtttctgg cgaaggccgt aacgtcgtgc
     7261 ccttctgcga tcgccctctc cacgaggcgc cgccccgtgc caccggtagg cccgaagatc
     7321 gccaacctca taggcatgca ccccgtcgct gaccgtggtg ctgccgcccg taagtgtatc
     7381 taatcgttcc aggcctttgc cttcgactcc agattagtgc gccgtcgttt tcggtatagc
     7441 tgctacgggc gatttcgaga aggagcggga agatctccga acgggatggt gcggccgagg
     7501 ccagccagaa cactgtaaac accaggcttc tactggccgt catgatctgt gcgctcttcg
     7561 tttcggtgtt cacgaacacc atggtcaacg tggccattcc cctgatccgg gagcagttcg
     7621 gcgcttcgga ggcccagtcc ggctgggtga taacaggcta cgcgctcacg ttcgcggtgg
     7681 gtatcccgct ctacgggcgc gtctcggacc tgttcagcct caggcgcaca ttcgccttcg
     7741 ggatggggat actcgccgtg ggcctggcgg tgtgcgccat tgcgccgacc cttaccctgc
     7801 tggtagcggg gagggccgtg cagggcgtgg gtgccagggc cataccggcc gtggccttcg
     7861 gttcgatcgc caagctgctc gcgcccggac agcgcggcac ggcgctcggc gcgctctcct
     7921 ccagcgtggg ggttggggct gccgccggcc cggtcctcgg cgggctgatc gcgggagcgg
     7981 ccggctggca ggcgttgttc tacgtaacct tcggcctgtc gttggtggtc ctggccgcct
     8041 ccatctacgt gcttcccgac aggatctccg acgagcccac ggaagacgcc acgttgcggg
     8101 ggctggacct gccgggcggc ctgctgctcg gtggtagcgc ggggcttctg ctcttcggcg
     8161 tcacccaggc cgaaacgatg gggctatcct ccattctggt ctggggctgc ctgctcctgg
     8221 ccgcgttcgc gggcgcctcg ttcgcgtggc atattcgcac cacccccgag cccttcatct
     8281 cgccgcggat cttcaccagc cgcgctttcc tggccgccgc cctggttggt ttcctggcgc
     8341 aattcgccaa cataggaagc ctctttctcg caccgctgct gctggagggg gcgggcggcc
     8401 tctcaccggc tgcggctggc ctcgtactgg cgccgggcgc gatcgccgtg gccatcctct
     8461 cccctttcgc tggcatgctc tccgataggc tcgggccgag aaccgtgctc ctcttcggcc
     8521 tcacgttcat gctcgtctcg accctcgttg tctcctcctt cgccgtcggt gcggcaccct
     8581 acatcctggc cggagggctc ttgctgctgg gtatcgggta cgcgggggcc aactcgccag
     8641 cggccaacgc cgcgtcagag gcggtaccac gcgaggcggc agggaccggt ctcggcatct
     8701 accagctgtt cttcttcctg ggttcggggg cggggccggc ggtgctcggc gcgtttctgg
     8761 cctcccgacg aggggcggat ggcggggcga tcaaccccct ctactccctg aaggccaccc
     8821 cgttctccga cgccttcctg ctggcggtcc tggccctgac gctggccctg ctggccacaa
     8881 cccggctcga cggcaaaccc gagggcgacg aataggaccc ggtcatcccg gcccacgaga
     8941 ggatcatcat ccagcggcac cggcttgcag cggttctaca agaggatgag ccatctcgaa
     9001 cgggaagaca tcgggccaca ggcttcaggc cacaggaaat gcttgctgaa actttatgct
     9061 tttcctctct tcgggtccac tctttccgca ggctactgta gtacgaccgg aagactcgtg
     9121 tccgcggaac aaggcacgga cccgttggtg tcccgatcga accacggaca cctgactttt
     9181 ccggaccctg ctcctctccc acttcgtgaa cccaggcatg tacaagaacg ttctactcgt
     9241 ctgctccgcg tgtggcgacg tggcaggcta cccggtggcc tggcgccagc tcctggaggg
     9301 tgggcacgac ccccaggcag cggggctcct cggcgcccgc ttcaccggag cgcagcaccg
     9361 ggcagcgggg gtgaaagcgg cagccgggtg ggatgtgggc cgggtccggt ggctcgccgg
     9421 tcaggacctc gtgggcgcgg cggcggccga tctcgggggt cacggcgagc agggatctcg
     9481 tgtaggggtg gagcgggtcg gtgagcacct cctggaccg
//
LOCUS       DBNGVD010000449         2710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2659516, whole genome shotgun sequence.
ACCESSION   DBNGVD010000449 DBNGVD010000000
VERSION     DBNGVD010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2659516"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..458
                     /gene="purE"
                     /locus_tag="AC16CM_12115"
     CDS             33..458
                     /gene="purE"
                     /locus_tag="AC16CM_12115"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3140624.1"
                     /translation="MSPSVGILVGSESDLPVLDKCTARLDGYGIEYELEVRSAHRNPE
                     GVSEYAGTARERGLKVIICAAGMAAHLAGAVAARTNLPVIGIPVASGSLSGFDALLST
                     VQMPSGVPVATVAVNGAANAGVLAAQILALSDPELADRL"
     gene            469..1782
                     /gene="purB"
                     /locus_tag="AC16CM_12120"
     CDS             469..1782
                     /gene="purB"
                     /locus_tag="AC16CM_12120"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3140625.1"
                     /translation="MIERYTLPEIGAVWTEEARYRAWLKVELAVCKARAQLGEIPTEE
                     VEELAEKADFTVERIQEVEAETNHDVIAFVTTVSEHAGDVARHFHFGLTSSDVLDTAG
                     ALQLRDALDLILEEAKNLTLLVAEMSLAHGDTVMVGRTHGVHAEPTTFGHKLAVWAFE
                     MERNLDRLSHAREIASVGKISGAVGTYANVDPKVESITCAELGLTSSPASTQVVQRDR
                     HAEVLAALAILGSTMEKVAVEIRGAQRTEVRELAEPFGRGQKGSSAMPHKRNPILAER
                     LSGMARILRANAAVGLENNALWQERDISHSSAERVVLPDSTILAFYMLRTTSRILRGL
                     QVDRDRMLENLNSGGGIVFSGRVLLALVESGMSRDDAYGVVQGAAMRAWEDDEDFRGL
                     LEENDEVRERLGDELDELFDPGYALRNLGVVSGRVEELIRRLEDG"
     gene            1775..2473
                     /gene="purC"
                     /locus_tag="AC16CM_12125"
     CDS             1775..2473
                     /gene="purC"
                     /locus_tag="AC16CM_12125"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3140626.1"
                     /translation="MAEDLLYEGKAKKVFTTDDEAVLLHRYKDDATAFNAQKRGSWKD
                     KGKTNATMSAAIFAYLETHGVPTHFLEQVDDTSITTKRLDMLPVEVVVRNVAAGSLSR
                     MLGFEEGRKLKAPIVELCYKDDDLGDPLCNWYHFRELGVSDEDLEFCEELGLKVNEVL
                     APFFDERGITLVDFKIEAGRDPEGNLMLADEISPDTCRFWDKETGEKLDKDRFRNDLG
                     GVEEAYAEMLRRVT"
     gene            2491..2703
                     /gene="purS"
                     /locus_tag="AC16CM_12130"
     CDS             2491..2703
                     /gene="purS"
                     /locus_tag="AC16CM_12130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3140627.1"
                     /translation="MLVRPKAGILDPQGEAVRSALPALGFEGVRTVHIGRLVEMEVES
                     ADEVDAMCRRLLSNPTIEEYEWEIVS"
BASE COUNT          527 a          842 c          900 g          441 t
ORIGIN      
        1 atagccctgg aagcgataga gctggaggac ctttgagccc gagcgtagga atcctggttg
       61 gtagcgagtc cgacctcccc gtactcgaca aatgtacagc ccgcctcgac ggctacggca
      121 tcgagtacga gctggaggta cgctccgccc accgcaaccc cgaaggcgtc tccgagtacg
      181 ccggcaccgc ccgcgagcgc ggcctcaagg tgataatctg cgccgccgga atggccgccc
      241 accttgccgg agcggttgcc gcacgcacga acctgccggt gatcggcatc cccgtggcct
      301 ctggctccct gagcggcttc gacgccctgc tctcgacggt ccagatgccg tctggcgtac
      361 cggtcgccac cgtcgccgtc aacggcgccg ccaacgccgg cgtcctggcg gcccagatcc
      421 tggccctctc cgaccccgaa ctcgccgaca ggctatagag ggacaggctt gatcgaacgc
      481 tacaccctgc cggagatagg ggcggtctgg accgaggagg ccaggtaccg cgcctggttg
      541 aaggtggagc tcgcggtctg caaggcaagg gcgcaactgg gggagatccc cacggaggaa
      601 gtggaggagc tggccgagaa ggcggacttc accgtggagc gcatccaaga ggtcgaggct
      661 gagacaaacc acgacgttat agccttcgtt accaccgtct cggagcatgc cggcgatgtg
      721 gcccgccact tccacttcgg cctgacgtcc tcggacgtgc tcgatacggc cggcgccctg
      781 caactgaggg acgccctgga cctcatcctg gaagaagcga aaaatctgac gcttcttgtg
      841 gcggagatgt cgcttgcgca cggggacacg gtaatggtgg gacggacgca cggcgttcat
      901 gccgagccga cgacgttcgg tcacaagctg gcggtgtggg ccttcgagat ggagcggaac
      961 ctggacaggc tctcgcacgc cagggagatc gcctcggtcg gcaagatatc cggcgccgtc
     1021 ggaacctacg ccaacgtcga cccgaaggtc gagtcgatca cctgcgcgga actcggcctc
     1081 acctcgtcgc ccgcctcgac gcaggtcgtc cagagagaca ggcacgcgga ggttctcgcc
     1141 gccctcgcca tactaggctc gacgatggag aaggtggccg tggagatccg tggcgcccag
     1201 cgcacggagg tccgggagct ggccgagccg ttcggacggg gtcagaaagg ctcatcggcg
     1261 atgccgcaca agcggaaccc gatcctggcc gagcgcctga gcgggatggc ccgcatcctg
     1321 cgggcgaacg ccgccgtagg cctcgagaac aacgccctct ggcaggagcg ggacatttcc
     1381 cactcctcgg ccgagcgtgt agtgctcccc gactccacca tcctggcctt ctacatgctc
     1441 cgcaccacct cccgtatctt gcgcggactg caggtggaca gagaccgcat gctggagaac
     1501 ctgaacagcg gcggcggcat agtcttctcc ggccgtgtcc tgctcgccct cgtggagagc
     1561 ggaatgagcc gcgacgacgc gtacggggtg gtacagggcg ccgcgatgcg ggcctgggag
     1621 gacgacgagg acttcagggg tctgctagag gagaacgatg aggtgcgcga gcgtctcgga
     1681 gacgagctgg acgaactttt cgatcccggc tacgccctgc gcaacctggg tgtggtgtcc
     1741 ggtcgggttg aagaactgat aaggaggttg gaagatggct gaagatctgc tgtacgaagg
     1801 caaggccaag aaggtcttta ccacggacga cgaggcggtt ctgttacacc gctacaagga
     1861 tgatgccacg gcgttcaacg cccagaagcg cgggtcgtgg aaggacaagg gcaagaccaa
     1921 cgcgaccatg agcgcggcga tcttcgccta cctcgaaacg catggcgtgc cgacccactt
     1981 cctcgagcag gtcgacgaca catccataac gacgaagcgg ctggacatgc tgcccgttga
     2041 ggtcgtggtc cgcaacgttg ccgccggttc cttgagccgc atgctcggct tcgaagaggg
     2101 ccgcaagcta aaggccccaa tagtagagct gtgctacaag gacgacgacc tcggggatcc
     2161 cctctgcaac tggtaccact tccgcgagct tggtgtctcg gacgaagacc tcgagttctg
     2221 cgaggagctc ggcctcaagg tcaacgaggt cctcgccccc ttcttcgacg agcgcggcat
     2281 aacgctcgtg gacttcaaga tagaggccgg aagggacccc gagggcaacc tcatgctggc
     2341 agatgagatc tcccccgaca cctgccgctt ctgggacaag gagaccggcg agaaactgga
     2401 caaggaccgc ttccgcaacg acctcggcgg cgtcgaggaa gcctacgccg agatgcttcg
     2461 tagggtgact tgaatagctt actaattcgt gtgctcgttc gtcccaaggc cggtatcctc
     2521 gatccgcagg gggaagctgt gaggagcgcg cttcctgcgc ttgggttcga aggtgtccgg
     2581 accgttcata tcggaaggct cgtcgagatg gaggtcgaga gcgcggacga ggttgacgcg
     2641 atgtgccgga ggctcctctc caacccgacg atcgaggagt acgagtggga gatcgtctct
     2701 tgaggatagg
//
LOCUS       DBNGVD010000450         3363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_473253, whole genome shotgun sequence.
ACCESSION   DBNGVD010000450 DBNGVD010000000
VERSION     DBNGVD010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_473253"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16CM_12135"
     CDS             <1..240
                     /locus_tag="AC16CM_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HIR3140628.1"
                     /translation="RGVPFDEGGAITRARRIGRLLVPIFISGFARADTLTVAMNTRSY
                     RGGRGRTKFRQMQARPSDWLVLILIIAWVIAAWMV"
     gene            586..1803
                     /locus_tag="AC16CM_12140"
     CDS             586..1803
                     /locus_tag="AC16CM_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3140629.1"
                     /translation="MTVTQAGKNADTDAFDSVAVQDFLGLDAQLSDEEKSVREGVRSF
                     VHEKIKPHIAAWYDGAVFPREIVPEFAELGLLGMHLTGYGCAGRSAVEYGLACMELEA
                     GDSGLRTFVSVQGSLAMSAIHKFGSEEQRQEWLPRMARGEAIGCFGLTEPTAGSDPAS
                     MKTFATRDGSDWVLNGEKRWIGLATIADVAVVWARTDEEKNPVRGFLVPTDSAGFSAR
                     DITPKLSMRASIQCDITLEDVRLPEGAMLPEARGLGGPFACLNEARYGIIWGAMGAAR
                     DSYESALAYAMEREQFGRPIASYQLVQEKLANMLIEVGKGTLLALHIGRMKDAGTLKT
                     EQISFGKLNNVREAIEIAREARTVFGGNGVTLDYPPMRHMSNLESVRTYEGTDEVHTL
                     ILGGAITGIPAFR"
     gene            2065..3039
                     /locus_tag="AC16CM_12145"
     CDS             2065..3039
                     /locus_tag="AC16CM_12145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016764124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3140630.1"
                     /translation="MKAITVEEFGEADVLRYVDADRPDPGEGEVLIEVRSAGVNYADT
                     MRRRNQYLEWQDLPFTPGSEVAGTVAEVGEGVDDVSVGDHVVSLLGTGGYAEYAVAPA
                     RGLIPLPEGLDFDRAAAIPLQGLTAYHCIKTSGALKDGESVLVHAAAGGVGTLAVQMA
                     KLLGAGTVIATASSEEKLDLARSLGADVLIDYTEDDWPQRVLEATEGNGADIILEMVG
                     ADFPQKNLECLAVFGRMVVFGAASGERGTIVPAALMKPCHAVVGFYLPQIMRRPDLFV
                     PSLEEVLGWISTDDLKLTIGDTYPLDQAATAHADLEGRKTTGKLLLNP"
     gene            complement(3215..3346)
                     /locus_tag="AC16CM_12150"
     CDS             complement(3215..3346)
                     /locus_tag="AC16CM_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3140631.1"
                     /translation="MPLKRFGRVEDIANVVAFLVSDDASYISGETIYVSGGPSSSVI"
BASE COUNT          676 a         1085 c         1064 g          538 t
ORIGIN      
        1 aggggagtcc ccttcgatga aggcggcgcc ataacccggg cacgccgcat aggacgcctg
       61 ctcgtcccca ttttcatcag cggcttcgca cgcgccgaca ccctcacggt agccatgaac
      121 acccgcagct accgcggcgg acgcggcaga accaagttcc gccagatgca agccaggcca
      181 tcagactggc tggttctaat cctcataatc gcgtgggtta tcgccgcgtg gatggtgtag
      241 agcacgctcc gccctacggg ctacgcttgt cagcacacta cggccttcgg cctccgtttg
      301 tcagcacgct cgggctttcg ccctcgcttg tcagcaacgc tccctacggt cgctttcagc
      361 cgatcacgct gcggtctgca cccttccgct ctgaagcttc gatgccggga agaggtgggt
      421 agtcgaagct gccgttacgg atcgagacga ccggctgaaa gcgaggcgaa gccgaagcgt
      481 gctgacaagc gagacccgca gggtcgatgc gtgctgacga gcggaggcga agccggagcg
      541 tgctaacatg cctgtgcgaa ggtcgggctc cgagagaggt ggaaaatgac cgttacgcag
      601 gctggcaaga acgccgatac cgatgctttc gattccgtgg cggtccagga ttttctgggc
      661 ctcgacgccc agctttcgga cgaagagaag agcgtcaggg agggggtgag gtctttcgtg
      721 cacgaaaaga tcaaacccca catcgcggcc tggtacgacg gggccgtttt tccgagggag
      781 atcgtgcccg agttcgccga gctcggcctg cttggcatgc acctcactgg ctacgggtgc
      841 gcgggcagga gcgccgtcga gtacgggctc gcctgcatgg agctcgaggc tggtgattcg
      901 ggattgagga ccttcgtgag cgtgcagggc tcgctcgcca tgagcgccat acacaagttc
      961 ggctctgagg agcagagaca ggagtggctc ccccggatgg ccagggggga ggcgatcggg
     1021 tgcttcggcc tgacggagcc gaccgccggc tcggaccccg caagcatgaa gaccttcgcc
     1081 acgagagacg gctccgactg ggtcctgaac ggggagaagc gttggatcgg ccttgcaacc
     1141 atcgccgacg tcgcggtggt ctgggcacgc accgatgagg agaagaaccc cgtccgcggc
     1201 tttctggttc caacggactc cgcgggcttc tcggccaggg acataacccc caagctctcc
     1261 atgcgagcct cgatccagtg cgacatcacc ctcgaagacg tccgcctccc cgagggcgct
     1321 atgctccccg aggcgagggg gctaggcggg cctttcgcct gcctcaacga ggcgaggtac
     1381 gggatcatct ggggcgcgat gggggcggcg cgcgactcct acgagtcggc acttgcttac
     1441 gcgatggagc gggagcagtt cgggaggccc atagcctctt accagctcgt gcaggagaag
     1501 ctcgcgaaca tgctcataga ggtgggcaag ggcacgctcc tcgcgctcca catcggtagg
     1561 atgaaagacg cgggcacgct caagaccgag cagatctcct tcggcaagct caacaacgta
     1621 agggaggcta tagagatagc ccgcgaagcc cgtaccgtgt tcggcggcaa cggcgtcacc
     1681 ctcgactatc ctcccatgcg ccacatgagc aacctggaga gcgtgagaac ctacgaagga
     1741 acggacgagg tccacaccct gatcctgggc ggagccataa ccggaatccc cgccttccgg
     1801 tagggcggat tcgaaacccg taatggcagg ttgaaacccg ccgcccgtcc gggcacgccc
     1861 catattgctc gattacaacg cacctgacat gtgaatgaat ccaccccacg ggggtgcacc
     1921 caaccgtatg gatacaaccc accccgcgcg ggtataaccc acacgatgta ggcgcggttc
     1981 gcgaaccgcc caacaacgca tcgtgacacg acaaagtgcg ttccaccaat tcgattctca
     2041 acgaacgggg gcggcatggc acagatgaag gcgataacgg tcgaggagtt cggtgaggcg
     2101 gatgtgctcc ggtacgtcga cgcggaccgc cccgacccgg gtgagggtga ggtcctgatc
     2161 gaggtccgct ccgccggcgt gaactacgca gacacgatgc gccgccgaaa ccagtacctg
     2221 gagtggcagg accttccctt cacgccaggc tccgaggtgg ccggcacggt cgccgaggtg
     2281 ggggaggggg tggacgatgt ctcggttggc gaccacgtgg tgagcctgct cgggacgggc
     2341 ggctacgccg agtacgccgt cgcgccggcc cgtggcctca tcccgctccc cgaaggcctt
     2401 gacttcgacc gggccgccgc catccccttg cagggcctga cggcctacca ctgcatcaag
     2461 acctcgggcg ccctgaaaga cggcgagagc gtcctggtcc acgccgcggc gggcggcgtc
     2521 ggtaccctcg ccgtccagat ggccaagcta ctcggggccg gcaccgtcat cgcaaccgcg
     2581 agcagcgagg agaagcttga cctcgcccgc tccctcggcg ccgacgtgct aatcgactac
     2641 acggaagacg actggccgca gcgggtgctc gaagctaccg agggcaacgg tgccgacatc
     2701 atcctggaga tggtcggcgc cgactttccc cagaagaacc tcgagtgcct ggccgtcttc
     2761 ggcaggatgg tggtcttcgg cgccgcgagc ggggagcgcg gaaccatcgt cccggccgcc
     2821 ctcatgaaac cctgccacgc cgtcgtcggc ttctacctgc cccagatcat gcgccgaccc
     2881 gacctcttcg taccctccct agaagaagtg ctgggctgga tctccacaga cgacctgaag
     2941 ctcaccatag gcgacacata cccactcgac caggccgcaa cagcccacgc cgacctggaa
     3001 ggacgcaaaa cgacgggtaa attactgctg aatccttgag agctagtagc tttcagcagt
     3061 cagctatcaa ctattaagtg aaacacgaga cgcccgaccg taaacggcaa tccgtcgcga
     3121 gccatcccct acaaaacccg tctgggcaac aagaagggcg gggatcctct ccccgcccaa
     3181 aaaagccgaa agctgacagc tagaactgac cgctctaaat aaccgagctg cttgggccac
     3241 ccgagacgta gatcgtctcg ccgctgatat acgaggcgtc gtcggagacg aggaaggcca
     3301 ccacgttggc gatgtcctca acgcgcccga agcgcttgag ggggatgttc ttctcgaacc
     3361 gtc
//
LOCUS       DBNGVD010000451         3126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3205363, whole genome shotgun sequence.
ACCESSION   DBNGVD010000451 DBNGVD010000000
VERSION     DBNGVD010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3205363"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="AC16CM_12155"
     CDS             complement(<1..596)
                     /locus_tag="AC16CM_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HIR3140632.1"
                     /translation="MAGLVGFGRHVVGNYFEHRMSTFAVALAYRGLFALFPFLLLLVM
                     FLGLFGPPDATDRLLEEAKAQQSERVPEQLEPVVEQMLVLLKPLEGMLDQAERQSRGG
                     LLVLGVAIALWSISALASTLADALNVANGVTETRSWWRTMALSLISGPIVALAVIVAV
                     VLMLTGTRVVEGMAEFLGVRGLFVFLWSWLHYPVALVLL"
     gene            904..1398
                     /locus_tag="AC16CM_12160"
     CDS             904..1398
                     /locus_tag="AC16CM_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3140633.1"
                     /translation="MGHQRVEPGPGQESVWDYPRPPRLEDVDKKVKVVFGGVTVAYTT
                     RAKRVLETSHPPVYYVPPEDIRMEHLRPSGGGSICEWKGRAGYYDLVTEERAEPRAAW
                     FYPDPTPPFASLTGYVAFYPSRMDGCWVGGEKVEAQEGDFYGGWITPDIVGPFKGAPG
                     TWGW"
     gene            1403..2038
                     /gene="pdxH"
                     /locus_tag="AC16CM_12165"
                     /pseudo
     CDS             1403..2038
                     /gene="pdxH"
                     /locus_tag="AC16CM_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
     gene            2164..2763
                     /locus_tag="AC16CM_12170"
     CDS             2164..2763
                     /locus_tag="AC16CM_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3140634.1"
                     /translation="MGDIGGWVLDVIAALGYVGLALLLIAENLFPPIPSEVVLPLAGF
                     LVGRGDLVFWQALLAATFGSVAGALILYALGRYGGRKLVLRYGRFLHVDEEGLDRADG
                     WFRRYGDWVVLFARVVPLARSVVSIPAGTMKMPAIRFTVLTAIGSLAWNTLLIGAGVI
                     LGANWQVVETWVGSYSNVVLLVAALVVATLLVVHGLRKS"
     gene            complement(2837..>3126)
                     /locus_tag="AC16CM_12175"
     CDS             complement(2837..>3126)
                     /locus_tag="AC16CM_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140635.1"
                     /translation="ASPRAPRIQVSAPQTPTGRFEISARLSNNRYNEVSFAVKAGEGR
                     FKPAGTDDNAPYRIFYDASNRKPGTTLTIKAVVDDLNGHLRSDSTTTTVGQ"
BASE COUNT          536 a          988 c         1034 g          568 t
ORIGIN      
        1 agcagcacga gggccaccgg gtagtgcagc cagctccaca gaaaaacgaa cagtccccgc
       61 acgccaagga attcggccat gccctctacc acccgtgtac ccgtcagcat cagcaccacg
      121 gcgacgatca cggccagtgc tacgatagga ccggagatca gggagagcgc catcgtcctc
      181 caccagctgc gcgtctccgt caccccgttc gcgacgttca gggcgtcggc cagggtgcta
      241 gccagcgccg agatggacca gagcgcgatc gcaacaccaa gcaccagcaa cccgcccctg
      301 ctctgtctct cggcctgatc caacatcccc tccagcggct tgaggagcac gagcatctgc
      361 tcgaccaccg gctccagctg ttccggaacg cgctcggatt gttgtgcttt ggcctcctcc
      421 aagagacggt ccgtggcgtc gggcgggccg aatagaccca gaaacatcac caggagcagc
      481 aggaaaggga acagcgcgaa caggccccgg taggccagcg ccaccgcgaa ggttgacatg
      541 cggtgttcga agtagttgcc gacgacgtgc ctgccgaacc ccacgagtcc ggccacgccc
      601 ccggagatgg aagacgatga agaagggagg ggcctggatc tcacgagctg gtatcgccct
      661 ttcctgaccg gcccgtcttg ctccgcgcgt ctccggcagg caggtcgatt acgggttcgt
      721 tcaacattag atgccaccct atctgcacgt cggttcccga attctacgcc agtacaggcc
      781 ccgcgggtaa cagagcgtgg ccgtcgcgct ctccgaccgc cccaggggat ggcgtgaaac
      841 caggcataca cggtagggtt cctcctccat atactcttgg ggcagaacag acgaggaggt
      901 cttatgggtc accagcgagt agagccgggt cccggtcagg aatccgtgtg ggattacccg
      961 cgcccgccga ggctcgagga cgtggacaag aaggtaaagg tggtcttcgg aggtgtaacc
     1021 gtcgcctaca caacgagggc caaacgtgtg ctcgagacga gccatccacc cgtgtactat
     1081 gttccccccg aggacataag gatggagcat ctacggccct ccggtggtgg ctctatctgt
     1141 gagtggaaag gacgggccgg ctactacgac ctcgtgacgg aggagcgggc ggaaccgcgg
     1201 gccgcgtggt tctacccgga tccgacgccg cctttcgcaa gcctcacggg gtacgtcgcc
     1261 ttctatccgt ccaggatgga cgggtgttgg gtaggagggg agaaggtcga ggcgcaggaa
     1321 ggagatttct acgggggttg gatcaccccc gacatagtag gacctttcaa gggcgcacca
     1381 ggcacctggg gctggtaggc gggtggacgc cgcgaacctc cgcagggagt acacaaggtc
     1441 cgggctggac gaagccacca tgcaacccga ccccatggtc cagttcgagg cgtggttcga
     1501 gaacgtcgtg gaagccaacc ttcacgagcc caacgccatg atccttgcca ccgcatctga
     1561 cgatggtcgt ccctccgccc ggaccgttct cctgaagggc tacgatgggc gcggtttcgt
     1621 cttctatacc aactacgagg ggcgcaaggc ccgcgatctg gaggccaacc cctcgtgcgc
     1681 cctcctcttc tactggggcg agctggagcg gcaggtcagg gtcgagggcc gcgccagccg
     1741 acttccttcc cgggagtcag acgcgtattt cgcaagccgt ccccggggca gccgcctcgg
     1801 ggcatgggcc tccgagcaga gccggccggt ccgggacagg agcgttctgg aggagcgcat
     1861 aaaggccctc gaggccgagt actaaggccg ggagatccca cgccccccct tctggggtgg
     1921 ctacagggtc gagcccgagg ccatcgagtt ctggcagggg cgcgagaaca ggctgcacga
     1981 caggatcgcc taccgtcgtg aggcgggcgt ctggaggcta gagcgactgc agccctgacg
     2041 gctgatctgg aggtcgggtt cgcctgggtc gtgcaacttt tccgggcgtg atcagtacca
     2101 tattggtgca ggcgtgacga tcgtggtgtc ccatgccggg catcgttgtg gaggtgttga
     2161 tctttgggag atatcggagg ctgggttctg gacgtgatcg cggccctcgg ctacgtgggc
     2221 ctcgcgttgc ttctcatcgc cgagaacctc ttccccccga taccgtccga ggtcgtcctg
     2281 ccgctcgcgg gttttctcgt ggggcgcgga gatctggtct tctggcaggc cctgctcgcc
     2341 gcgaccttcg gcagcgtcgc gggggccctt atcctctacg cccttggccg ctacggggga
     2401 cgcaagctcg tgcttcgcta cgggaggttc ctgcatgtgg atgaggaagg gctggaccgg
     2461 gccgacggct ggttccggcg ctacggagac tgggtggtgc tcttcgcccg cgtcgtgccc
     2521 ctcgcgcgga gcgttgtctc aatacccgcc ggcaccatga agatgccggc catccggttc
     2581 accgtgctga ccgccatcgg ttccctcgcc tggaacacgc tgctcatagg ggccggcgtc
     2641 atcctcgggg ccaactggca agtggtcgaa acctgggtcg gctcctactc taacgtcgtc
     2701 ctcctcgtcg ccgcactcgt cgtcgccacc ctgctagtcg tgcacgggct ccgcaagagc
     2761 tagggaaggc tacaggtgtc aggtaattct cctgacacct gttacctgat gcctgtagcc
     2821 tttttctcga accgctctac tggcctacgg tggttgtcgt cgagtcggag cgcagatgcc
     2881 cgttcaggtc gtcgacgacc gccttgatgg tgagggtcgt gcccggcttc cggttgctgg
     2941 cgtcgtagaa gatgcggtac ggggcgttgt cgtccgtgcc ggccggtttg aacctgcctt
     3001 cgccggcctt gacggcgaag ctcacctcgt tgtagcggtt gttggagagg cgggcggaga
     3061 tctcgaagcg tcccgtgggc gtctgggggg cgctcacctg tatccggggc gcccgcgggc
     3121 ttgcag
//
LOCUS       DBNGVD010000452         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3205478, whole genome shotgun sequence.
ACCESSION   DBNGVD010000452 DBNGVD010000000
VERSION     DBNGVD010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3205478"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..146
                     /locus_tag="AC16CM_12180"
     CDS             6..146
                     /locus_tag="AC16CM_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HIR3140636.1"
                     /translation="MSGENAYDRYLAVHTTTHPGKKVMTRGEFYRWRQDEKYSNPGAR
                     CP"
     gene            complement(184..996)
                     /locus_tag="AC16CM_12185"
     CDS             complement(184..996)
                     /locus_tag="AC16CM_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140637.1"
                     /translation="MSRLRTDELTLAYDEKVIIKDLTIEVPEGAVTSVVGPNGCGKST
                     LLRSLARLMRPRGGAVYLDGAAISKLPTREVARRLGILPQSPVSPEGLTVSELAAQGR
                     YPHQGWLRQWSRADGQAVERALQTTGVAELADRPLDTLSGGQRQRAWISMALAQETET
                     LLLDEPTTFLDMAHQLEVLQLLARLNREEGRTILMVLHDLNNAARYSHHMVALAGGGV
                     YDAGAPEEVVTPDLLREVFGVEADVVPDPRTGSPLCIPQGLHDRASKLRAER"
     gene            complement(993..>1707)
                     /locus_tag="AC16CM_12190"
     CDS             complement(993..>1707)
                     /locus_tag="AC16CM_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140638.1"
                     /translation="VTDLDLLDDAMRRMGQTYGLENRAFAGTFLLRDLLWTVLAPSVA
                     VFLSERRLPDLDAENVAVRVGEDGFCRDVAFLGSRFLALPEDPEAWHPDALVVPIEDV
                     LDVGVRIPVSETYLQDLIPALRGLRVRRGTRLLGRAAADVCVEAFIYVGRGLGQEEEG
                     LELATRLLAGPPPLRAPLDYRVIEFPGGSETAPIRNACCLYYKTGAGTCFTCPRKTDE
                     ERARQLSEAADVPREDEGS"
BASE COUNT          273 a          583 c          575 g          276 t
ORIGIN      
        1 aggagatctc cggggagaac gcctacgacc gctacctggc ggtccacacc acgacccacc
       61 ccggcaagaa ggtcatgacc cgcggcgagt tctaccgttg gcgccaggac gagaagtaca
      121 gcaacccggg ggcccgatgt ccgtgatgac gcccagccgg ccggtcggcc ggcacggcag
      181 ttcctagcgc tcggctctca acttactggc ccggtcgtgg aggccctgcg gtatgcagag
      241 cggggaaccg gtgcgcggat cggggaccac atccgcctcg acgccgaaga cctctctcaa
      301 gagatctggt gtgacgacct cttctggtgc tcccgcgtcg tagaccccgc cgcccgcgag
      361 ggcaaccatg tggtgcgagt acctggctgc gttgttgaga tcgtggagga ccatgaggat
      421 ggtccttccc tcctcgcggt tcaggcgcgc gaggagctgg agaacctcca gctggtgggc
      481 catgtccagg aaggtcgttg gctcgtcgag cagcagggtc tccgtctcct gtgccagggc
      541 catggagatc caggcccgct ggcgctggcc gcccgagagc gtgtcgaggg gacggtcggc
      601 cagctccgcg acgccggtcg tctggagcgc ccgctctacg gcctgcccgt ccgccctgga
      661 ccactgcctg agccaaccct ggtgtgggta acggccctgg gcggccagct cgctcaccgt
      721 cagcccctca ggtgaaacgg ggctctgggg tagtatgccc agtcgtctgg caacctcgcg
      781 ggtgggcagc ttcgagatag cggcgccgtc gaggtatacg gcgccaccgc gcggccgcat
      841 gaggcgggcg agcgagcgca ggagggtgct cttgccacag ccgtttgggc caacgacgga
      901 ggtgaccgcg ccctccggca cctctatggt caggtctttt atgatgacct tctcgtcgta
      961 ggcgagcgtc aactcgtccg ttctgagcct gctcatgatc cctcgtcttc ccgcggtaca
     1021 tccgccgctt ccgacagctg tcgcgcccgt tcctcgtccg tctttcgggg gcaggtgaag
     1081 caggtgccgg ccccggtctt gtagtacagg cagcacgcgt tcctgatcgg cgcggtctcc
     1141 gaccccccgg ggaactctat cacgcggtag tcgagcggcg cgcggagggg aggcgggccc
     1201 gccagcagcc tggtggcaag ctcgagaccc tcctcttcct gcccgagccc gcggccgacg
     1261 tagatgaagg cctcgacgca cacgtccgcc gccgcccgac cgagcaaccg cgttcctcgt
     1321 ctaacgcgca accctctcag ggccgggatc aggtcttgca gatacgtctc tgacacgggt
     1381 atccgtaccc cgacatcaag cacgtcctcg atgggcacga cgagcgcgtc cgggtgccag
     1441 gcctccgggt cttcgggcaa cgccaggaag cgagacccga gaaaggcaac gtcccggcag
     1501 aagccgtcct caccgacgcg cacggccacg ttctcggcgt ccaggtcggg gagccggcgt
     1561 tcggacagga ataccgcgac ggagggggcg agcaccgtcc agagaagatc cctgagcagg
     1621 aaggttcccg cgaaggcccg gttctccagg ccgtaagtct gccccatgcg ccgcatggcg
     1681 tcgtcgagca ggtcgaggtc cgttacg
//
LOCUS       DBNGVD010000453         5879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3387723, whole genome shotgun sequence.
ACCESSION   DBNGVD010000453 DBNGVD010000000
VERSION     DBNGVD010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3387723"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC16CM_12195"
     CDS             complement(<1..338)
                     /locus_tag="AC16CM_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3140639.1"
                     /translation="MDNKTGAVVVLRKIHKSFGDHEVLKGVDLDVSSGECVVIFGRSG
                     SGKSTLLRCINLLEEPTGGSVAVGGVIYEAGPSNHQKRRRVREIRTRTGMVFQEFNLF
                     PHLSVIENVI"
     gene            complement(328..1065)
                     /locus_tag="AC16CM_12200"
     CDS             complement(328..1065)
                     /locus_tag="AC16CM_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3140640.1"
                     /translation="MLGILFAAFQFNTSFMLDWLPFILGGVSLTILVSVLGILLSIPL
                     ALLGALGRLSKNPVYYGISSFYVSFIRGTPLIVQIFFVYLGLPQLAQYAPPQLQGLFI
                     LGTVTSGVLALGVNYGAYMAEIFRAGIQSVGHGQIEAAQALGMTRSQTMRRIVLPQAV
                     RVIIPPTGNEFIAMIKDSSLVGIVGTQEVFFRASKIGRQYFQNLETLVVAALIYWILT
                     SIFSYFQGRLERRMERGYVRDGGPRGQ"
     gene            complement(1180..2067)
                     /locus_tag="AC16CM_12205"
     CDS             complement(1180..2067)
                     /locus_tag="AC16CM_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3140641.1"
                     /translation="MNPKRHLGRLASLAVAILAVLAITACGGGGSSSGGDGGGNLLQE
                     VKDRGTLRVSTDPAYPPQSFQANDGTFKGFDIDVAEEIAKRMGVEVQWITPSWDVITA
                     GNWNGRWDISVGSMTVTPERAKVLYFSSPYYYNPAAAAVHEDNTDITDVETDLDGKTI
                     GVCSACTYEFYLDGTLNIPGEDYDFVVDDPQIKTYDTDSSAIQDLALGDGVRLDAAMS
                     SLTTLQEAEKKGTPIKVIGDPLYYEPLAVAIDKEAPGNPKPLVDEVDKIVKEMHKDGT
                     LSKLSEKWYGYDLSKKQGS"
     gene            complement(2116..3765)
                     /locus_tag="AC16CM_12210"
     CDS             complement(2116..3765)
                     /locus_tag="AC16CM_12210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3140642.1"
                     /translation="MVSNGLRYDAVVVGGGHNGLVCAAYLARAGIKTLVLERRHLVGG
                     AAVSEEVWPGYKVSTASYVVSLMSDVVVRDLDLPRYGYHVYPLDPAYFAPFPDGSGFL
                     LWDDPAEAAREIGKISERDGKAYLRYSSDLGELADLVRPLLYRRPPDPGVRSFADLVE
                     TLSLGRYAFGNRKNISRLVDLMTMSCADFLDRYFVDDRIKGALAPGGVIGMWGGPMSP
                     GSAYVLLHHRMGEVDGVTGGWGFVRGGMGGVSEAIAASARAAGAEIRTDSEVASIDVS
                     GGRATGVTLQDGTTIEAGTVISGAHPQTTLLSLLGAEHLPGEMVDELSTFRNRSPSAK
                     VNLALSELPDLTAKPGTELGPQHPEIMISPSLEYLERAWDDAKYGRFSEGPMIDAVIP
                     TTKDPTIAPEGRHIMTCFVQYAPYEPREGSWDDGAREALGDTVVETINQYAPNFKDSV
                     IERQVLTPYDLEQRFGLIGGNIFQGEMSLDQLFSFRPTPELSGYRTPVEGLYLCGSGT
                     HPGGGVMGIPGLNASRVVAADHKSAERKKRFRERLTSYSRR"
     gene            3868..4794
                     /locus_tag="AC16CM_12215"
     CDS             3868..4794
                     /locus_tag="AC16CM_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HIR3140643.1"
                     /translation="MTPISRELLAPSLNRFGRSLTLPAAAYIDLSVFRWEQEHFFDEA
                     WVCAGRTGSEGASDGRTVEWNGWTFVNAGGEAPPFEDHVGNLGGLIRNYDCAELVVGA
                     RHDYEISANWKILAENYHECYHCSSIHPELCEVSPPTSGGDNLEPEGAWIGGPMNLYE
                     GVETMSLDGKSHAPLLPNLEEGQDRTVHYFQLFPNLLISPHPDYVMTHRLIPLAPDRT
                     AVECEWLFSRGAVERDDFDPSYASDFWDITNRQDWHACESVQRGASSRGYRQGPLSPR
                     EDAVYQLITMVANGYLEGRVTRPASRVAEAPA"
     gene            complement(4887..>5879)
                     /locus_tag="AC16CM_12220"
     CDS             complement(4887..>5879)
                     /locus_tag="AC16CM_12220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3140644.1"
                     /translation="EGGAQEAGGGANGRADGNFRASPIVRRLANENDLDLAKIDGSGP
                     AGRIVERDVRAAMESGTAQRDQDGAQEEVEAPEEPQQPQAEMQGFQPARMPEPTDEPG
                     TQLVEPTRMMQVIGERMTESKQHVPHFYATVEVRMDAAMALRKQLNEQLEDEGIKLSV
                     NDFVMKACAVALRNYPNLNALYTTRGVELHEKVDMAMAVALDQGLITPVIRDIGSKGL
                     SAISQESKDLASRARDGKLQPDEYQGGTITVSNMGMFGIESFTAIINPPQAAIVAVSS
                     IDRRPSYDENGEIVPGSFMKLTLSADHRIANGRDGALYMAEVKRVIENPVMLMV"
BASE COUNT         1010 a         1928 c         1873 g         1068 t
ORIGIN      
        1 tcgatgacgt tctctatcac gctcaggtgg gggaagaggt tgaactcctg gaacaccatg
       61 ccggtgcggg tccggatctc acgcaccctc ctccgcttct ggtggttgga gggcccggcc
      121 tcgtagatca cgcccccgac cgccacgctc ccgcccgtcg gctcttccag aaggtttatg
      181 caccggagga gcgtactctt ccccgagcct gagcggccga agataaccac gcactccccg
      241 ctcgacacgt ccaggtcgac gcccttcagc acctcgtggt ccccgaagga cttgtggatc
      301 ttgcgcagga cgacgacagc tccggtctta ttgtccacgc ggaccaccat ccctcacgta
      361 gcccctctcc attctcctct ccaaacgtcc ctggaagtag gagaagatgc tcgtgaggat
      421 ccagtagatc agggccgcca ccaccagcgt ctccaggttc tggaagtact ggcgcccgat
      481 cttggaggcg cggaagaaga cctcctgggt tccgacgata ccgacgaggg aggagtcctt
      541 gatcatggcg atgaactcgt tgccggtcgg cgggatgatg accctgacgg cctggggaag
      601 cactatgcgg cgcatggtct gcgaacgcgt catcccgagg gcctgcgccg cctcgatctg
      661 cccgtgtccc accgactgga tgccggcgcg gaagatctcc gccatatagg ctccgtagtt
      721 gaccccgagc gccagtacgc cggaggtgac cgtcccgaga atgaagaggc cctgcaactg
      781 cggcggcgcg tactgggcaa gctgcgggag gccgaggtag acaaagaaga tctgcacgat
      841 caagggcgtc ccccgaatga aggagacgta gaaactgctg atcccatagt agacggggtt
      901 cttggatagt cgtcccaggg ctccgagaag cgcgagcggg atggaaagca ggataccaag
      961 gacggaaacg agaatagtaa gcgaaacgcc gccgagtatg aacggcagcc aatccagcat
     1021 gaacgaggtg ttgaactgga aggccgcgaa caggatcccg agcacgatga agatgccaac
     1081 ccatacggcg gccagctaga tacggaaggg gatacgggcg gaacgggcct ccagggcatc
     1141 ccttatgttg gagaacgtcg agatgctaac cccggaagac taggagccct gcttcttgct
     1201 caggtcgtag ccgtaccact tctcggagag cttcgagagc gtgccgtcct tgtgcatctc
     1261 cttgacgatc ttgtccactt catcgaccag cggcttgggg ttgccgggag cctccttgtc
     1321 tatggccacg gcgagcggct cgtagtagag cgggtcgcct atgaccttta tgggtgtccc
     1381 cttcttctcg gcttcctgga gcgtggtcag ggaggacatc gccgcgtcca gcctcacgcc
     1441 gtctcccagg gcgaggtcct gtatggccga ggagtcggtg tcgtaggtct ttatctgcgg
     1501 gtcgtcgacg acgaagtcgt agtcttcccc gggtatgttg agcgtgccgt cgaggtagaa
     1561 ctcgtaggtg caggccgagc atacgccgat ggtcttgccg tcgagatcgg tctccacatc
     1621 ggtgatgtcg gtgttgtcct cgtgcaccgc cgcggccgcc gggttgtagt agtagggcga
     1681 ggagaagtag agcaccttcg cccgttccgg ggtgacggtc atcgagccca cgcttatatc
     1741 ccagcgtccg ttccagttgc ccgcggtgat cacgtcccag gacggggtga tccactgcac
     1801 ctccacaccc atccgcttgg cgatctcctc ggccacgtcg atgtcgaagc ccttgaacgt
     1861 gccatcgttg gcctggaagg actgcggcgg atacgccggg tcagtcgaga ccctgagcgt
     1921 gccgcggtcc ttgacctcct gaaggaggtt tcctcccccg tcgccgccgc tcgacgagcc
     1981 gcctcccccg caggcggtga tggccagcac ggccagaatc gcgacggcca gcgacgccag
     2041 ccttcctaag tgcctcttcg ggttcatgct ttactccgtt cccgcggtac cccttcggat
     2101 cgacactata gctggtcagc ggcgcgaata cgaggttaga cgctctctga acctcttctt
     2161 gcgctccgcg ctcttgtggt cggccgccac caccctggag gcgttcaggc cgggtatgcc
     2221 catgaccccg ccaccggggt gggtgcccga gccgcagagg tagagcccct ccacgggcgt
     2281 cctgtagccg gagagctccg gggtggggcg gaaggagaag agctgatcga gcgacatctc
     2341 cccctggaag atgttgcccc cgatcaggcc gaagcgttgc tcgaggtcgt agggcgtgag
     2401 tacctggcgt tcgatcaccg agtctttgaa gttgggcgcg tactggttga tggtctcaac
     2461 caccgtgtcg ccaagtgcct ccctggcgcc gtcgtcccag ctgccctccc ttggctcgta
     2521 aggggcgtac tgcacgaagc aggtcatgat gtgccgacct tctggggcga tggtcgggtc
     2581 cttggtggtt gggatcacgg cgtcgatcat cggcccttcc gagaaccggc cgtacttggc
     2641 gtcgtcccac gccctctcca ggtactccag agaggggctg atcatgatct ccgggtgctg
     2701 tggcccgagc tccgtgcctg gtttggccgt gaggtcggga agctcggaga gcgcgaggtt
     2761 gaccttggcc gatgggctac ggttcctgaa cgtcgagagc tcatccacca tctcgccagg
     2821 caggtgctcg gccccgagga gcgagaggag ggtggtctgg ggatgcgccc cggagatgac
     2881 cgtcccggcc tctatcgtgg tgccgtcctg aagcgtcacg ccggtcgccc ggcccccaga
     2941 gacgtcgatg gaggcgacct cagagtcggt gcggatctcc gcgcccgcag cccgggcaga
     3001 ggcggctatg gcttcggaga cgccgcccat gccgccgcgc acgaagcccc agccgccggt
     3061 cacgccgtcg acctcgccca tcctgtggtg cagcagcacg taggccgaac cggggctcat
     3121 cggcccgccc cacatgccga tcaccccgcc aggggcgagc gcgcccttga tcctgtcgtc
     3181 cacgaaatag cggtctagaa agtccgcgca gctcatggtc atcaggtcaa cgagccgcga
     3241 gatattcttg cggttgccga aggcgtagcg cccgagcgag agcgtctcga ccaggtcggc
     3301 gaaggaacgt acccctgggt cggggggcct cctgtagagt agcggcctca cgaggtcggc
     3361 cagctcgccg agatcgctgc tgtacctcag gtaagccttg ccatcccgct cgctgatctt
     3421 gcctatctcc ctggccgcct ctgccgggtc gtcccagagc agaaagccag acccgtcggg
     3481 gaagggggcg aagtaggcgg gatccagggg atagacgtgg tagccgtacc tggggagatc
     3541 caggtccctc accaccacgt cggacatgag gctgaccacg taggaggccg tcgagacctt
     3601 gtagccgggc catacctcct cggataccgc tgcgcccccg acgaggtgcc gccgctcgag
     3661 cacgagcgtc ttgatgccgg cgcgggcgag gtacgccgcg cacacgagcc cgttgtgtcc
     3721 cccgccgacc accacggcgt catagcgcaa tccgttcgag accatgatct tccttcccga
     3781 ctcaagcccg tttcctgtac aattgccgca ttctagcagc aagtgcctgc cacacgggcc
     3841 gcgggtccgt acggagggga ggaccacgtg acacccatat cccgcgagct tctagcacct
     3901 tcgctaaaca ggttcggacg cagcctcacc ctccccgcag cggcctacat agatctgtcc
     3961 gtgttccggt gggaacagga gcacttcttc gacgaggcct gggtctgcgc cggccgaacc
     4021 gggtccgagg gcgccagcga cgggcgcacc gtggaatgga acggctggac cttcgtcaac
     4081 gccgggggcg aagccccacc gtttgaggac cacgtcggca acctgggagg gttgatccgg
     4141 aactacgact gcgccgagct cgtcgtgggg gcacgccacg actacgagat ctccgccaac
     4201 tggaagatcc tggccgagaa ctaccacgag tgctaccact gctcctcaat ccaccccgag
     4261 ctctgcgagg tgagcccgcc aaccagcggc ggcgacaacc tggagcccga gggggcctgg
     4321 atcgggggcc caatgaacct ctacgagggc gtcgagacca tgtcgcttga cggcaagagc
     4381 cacgcgccgc tgctgccgaa cctcgaagag gggcaggacc gcaccgtcca ttacttccag
     4441 ctcttcccca acctcctcat aagcccccac cccgactacg tgatgaccca ccggctgata
     4501 ccgctcgccc cggacaggac cgccgtggaa tgcgagtggc tcttctcccg cggggccgtc
     4561 gagcgggatg acttcgaccc ctcctacgca agcgatttct gggacataac caaccgccag
     4621 gactggcacg cctgcgagtc cgttcagcgc ggcgcgtcat cccgcggcta ccgccagggg
     4681 ccactctccc cccgcgagga cgccgtctac cagctcatca ccatggtggc caacggctac
     4741 ctggaaggca gggtcacaag gcccgcgagc cgggtagccg aggcgccggc gtagtagcta
     4801 ttagcgatca gcggtcagct atcagccaaa gaacaataag ctgatagcta accgctgaaa
     4861 gctgacggcg cggttcgcga accgccctag accatcaaca ttacggggtt ctcgatcacc
     4921 cgcttgacct ctgccatgta gagggctccg tcgcggccgt tggctatgcg gtggtcggcc
     4981 gagagggtga gcttcatgaa gcttcccggc acgatctcac cgttctcgtc gtagctcggg
     5041 cgacggtcga tggagctgac cgcaacgatg gcagcctgcg gcgggtttat gatggcggtg
     5101 aagctctcga tgccgaacat acccatgttg gagacggtga tggtgccacc ctggtactcg
     5161 tcgggctgca gcttgccgtc gcgcgccctc gaggccaggt ctttcgactc ctgggagatg
     5221 gcggagaggc ccttgcttcc gatatccctt atgacggggg ttatgaggcc ctggtcgagg
     5281 gccacggcca tggccatatc caccttctcg tgcaactcga cgccgcgggt ggtgtagagg
     5341 gcgttgaggt tggggtagtt cctgagggcg accgcgcagg ccttcatcac gaagtcgttg
     5401 acggagagct tgatgccctc gtcctcgagc tgctcgttga gctgcttcct gagggccatc
     5461 gcggcatcca tcctgacctc cacggtggcg tagaagtgcg ggacgtgctg cttagattcg
     5521 gtcattcgct cgccgatgac ctgcatcatg cgcgtgggct cgacgagctg cgttccgggc
     5581 tcgtcggtcg gctctggcat gcgggccggc tggaagccct gcatctcggc ctgcggctgc
     5641 tgcggctcct ccggcgcctc cacctcttcc tgcgccccgt cctggtcccg ctgggccgta
     5701 ccgctctcca tggcggcccg cacgtcgcgc tccacgatgc gtcctgccgg gcccgagccg
     5761 tctatcttgg cgaggtccag gtcgttctcg tttgccagcc tgcggactat gggagaggcc
     5821 ctgaagttcc cgtccgccct gccattagcg ccaccaccgg cctcctgggc gccgccttc
//
LOCUS       DBNGVD010000454         6390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4844504, whole genome shotgun sequence.
ACCESSION   DBNGVD010000454 DBNGVD010000000
VERSION     DBNGVD010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4844504"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..2860
                     /locus_tag="AC16CM_12225"
     CDS             635..2860
                     /locus_tag="AC16CM_12225"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3140645.1"
                     /translation="MILRPKLRAPVPRPEQLVRDGLLALLHDALECRVSVISGPTGYG
                     KTTLLAHWLQAEDGELPFAWVSLDEHDNDPARLWGHIVEALRGAMPKEEDFGADVLVG
                     LGAVGEAFVGSTLSMLINALAESPHQIVLLLDDYQFVTEWETHESIAFFIEHIPENVH
                     LVISSRSDPPLPLGRLRAMGELNEIRTGQLAFTEEEAECLLNEKMGLGIDADDVAILL
                     ERTEGWPAGLYLASLSLRNKEDKHAFIQSFRGSDRYIVGLLGEEVLAHLSAEVRQFLL
                     ETSVLRRLTGPLCDAVTGRQDSANVLRELARSNLFVVGLDDQDQWYRYHHLFCELLLY
                     ELKSVQPGMEPDLRARASEWLEDAGMIEEAVWQAFEAENHGLAGLLIARHWRGYLFAG
                     QTATVQRWLSSLPQDVLAHSAPLTLVKAWICALDGQREESQRFLRLAESFGYEGPLPD
                     GIASVESGVALLEATFGYGGIRHGVQTARLAAELEPIETSPWAVVACLALGSSLYLQG
                     DLPEARRALENALSLADDNQRLVRVVALSFLSFVATDEGHLEEAESLALAAQALVERS
                     RPYRIPQTTLAPIAYGRVLAEKGNLEEAQEELEGALSSRGRLPGLSPWPNLIGLLALA
                     PVLAGRGDRDGARAALAEVRAILDAHPDAGMLPELLERQNRKLRVRRAREGRLDAELT
                     ERELEVIGLLDGRLTTRQMAESLFVAPSTVRTQIKSIYRKLGVSSRGEAVAVAREQGL
                     V"
     gene            3014..3229
                     /locus_tag="AC16CM_12230"
     CDS             3014..3229
                     /locus_tag="AC16CM_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140646.1"
                     /translation="MRSELGNVFAAAFEGMEVEARDGETVLTGRITDQPQLYGILERI
                     NGLGLELLSVQALTDDANQSSIVNRRP"
     gene            3241..3870
                     /locus_tag="AC16CM_12235"
     CDS             3241..3870
                     /locus_tag="AC16CM_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HIR3140647.1"
                     /translation="MSLAVLPLAITMNAGPQIMSALIFVTASKPIKLSAYFLTGVVIA
                     VTAGVTITFGIASVFGNGISLGDSSDSGSVGNIIQYLLVGLLVVASIRSYLTRKTSEP
                     PRWLGAMQNAEPKQAFLAGLLLLSVFPSDFVVLMTVGVNLVQKDASLLHALPFVAATI
                     LIAALPMLSYLLFRRRAEVLMPKLRDWMNTNSWLVNIIVYIVFILLIVS"
     gene            3900..5288
                     /gene="gad"
                     /locus_tag="AC16CM_12240"
     CDS             3900..5288
                     /gene="gad"
                     /locus_tag="AC16CM_12240"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - GABA shunt [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HIR3140648.1"
                     /translation="MGLHRKVDGDGLDGRYLSVSPLLDGKVTVPKHRLPDGPMLPEMA
                     YEIIHDELMLDGNARLNLATFVTTWMEPQAERLMAEALDKNMIDKDEYPQTAELEMRC
                     VNMLSRLWNSPSTDQATGCSTTGSSEAAMLGGLALKRRWQQKRRAAGESTERPNMVMG
                     VNVQICWEKFCNYFEVEPRYVPMEGDRFHLNGEEAVALCDENTIGVVAVLGSTFDGSY
                     EPVAEISDALDDLEDRTGLDVPIHVDAASGGMVAPFIDPDLVWDFRLPRVASINTSGH
                     KYGRVYPGVGWAIWRDAEALPEDLVFRVNYLGGDMLTFALNFSRPGSQIVAQYYNFIR
                     LGFEGYRRVQQECRDIATLGSAAIAELGPFELITDGSELPVFAFKVRDDVSAFSAFDV
                     SGGMRERGWQVPAYSFPENREDLVALRVVVRNGYSRDPADMFLDDLKRLLTRLEKQSS
                     PTHDESYSGFAH"
     gene            5382..>6390
                     /locus_tag="AC16CM_12245"
     CDS             5382..>6390
                     /locus_tag="AC16CM_12245"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3140649.1"
                     /translation="MSQDRELRNEDQGVPDYAAQRGARLEGILGRVSDAPLRQNNGIS
                     LLKNGPDTYDDWLTAISRAKRWIHLDNYIIANDEVGNRFARAMSAKAREGVRVRVLHD
                     WFGCLDVPRSFWRGMREAGVEVKAVNPPASGPPLGAIRRDHRKLLVVDGEYASTGGVC
                     IADGWLVRSPDTGLPYRDTAVSVRGPIVADVNRAFTSLWSEQVGELPEDERPDPEGIP
                     TAGETAARLVVQEPRRMRTLRMLELLTAGVQERLWITDPYFLSMPILTQSLMATARDG
                     VDVRVLNPATVDIAWIGRASRAGYRQFLEAGVRIFEYGGPMIHAKTLVADGWLSKVGS
                     TN"
BASE COUNT         1196 a         1973 c         2081 g         1140 t
ORIGIN      
        1 gtccccccga acgccaggat cgcactcaag agctcgaagc ctgcaaccgt aaacatcatg
       61 acggccgtga aggtgaacca cccgggcctg tgcgtcaccg cgccgcctgc agggctcatg
      121 gggtcactcg ctcttcccgg catcggttcg ctgtgctgcg ccatcgctat tctcctctcg
      181 gtctctagcc atctcgggct aatgctcggc tgatcgcctt ctggatttcc tggttctcta
      241 tctccgtttt cgtacgctcc gtttgttcca gggccgccca cacggccatc gcctcgttcc
      301 catcatccga ccctacacct gatgcttcat cacccaacac gccgtttccc ctaggggatt
      361 tgctggctgc caggccacat aagaatcctg gtcaagaatc gtattccccg gcggttcctg
      421 gtagagacat cgccggtagt cagaattggc aagtcaggtg gagcacggga aggtgcgaga
      481 acggttggat cccctctttc agatggcgtc gagcatcgcc ggacggggcg tgcggagagg
      541 ctgtccgaac ttctgtgggg ccttataatg gtaagaagga gcagaggaga gggtaatagg
      601 cgcgtcgggt gagccacgca atgatcctga atgggtgatc ctaaggccca agctacgggc
      661 gccggtccct cgccccgagc agttggtccg ggacgggctg ctcgcgctgc tccatgacgc
      721 gctcgagtgc agggtcagcg tcataagtgg tccgacaggc tacggcaaga ccacgcttct
      781 agcccactgg ctacaggccg aagacgggga gttgcccttc gcctgggtct cattagatga
      841 gcacgacaac gatccagccc ggctgtgggg gcacatagtc gaggcgttgc gcggggccat
      901 gccgaaggaa gaggacttcg gggccgacgt tctcgtggga ttgggtgccg tgggagaggc
      961 gttcgttggg agcacgctct ccatgctaat aaacgcgctc gccgagtcgc cccatcagat
     1021 tgtgctgttg ctcgacgact accagttcgt aaccgaatgg gaaacccacg agtcgatcgc
     1081 cttctttata gagcacatcc ctgagaacgt tcacctcgtc atctccagcc gatccgaccc
     1141 gccgctaccg ctgggacggc taagggccat gggggagctg aacgagatcc gtaccggtca
     1201 gctcgccttc accgaggagg aggccgagtg tctgctcaac gagaagatgg ggctcggcat
     1261 cgacgccgat gacgtcgcca ttttgctcga gcgtacggag ggatggccgg cgggactgta
     1321 cctggcctcc ctctccttgc ggaacaagga ggacaagcac gcctttatcc aatctttcag
     1381 gggctccgac cgttacatag tgggccttct gggagaggag gtcctggctc acctctccgc
     1441 ggaggtgagg cagttcctgc tcgagacctc ggtactgagg aggctgacgg ggccactctg
     1501 cgacgccgtg accggcaggc aggattcagc caacgttctg cgcgagctgg cacggtccaa
     1561 cctgttcgtg gtcggcctgg acgatcagga ccagtggtac cgttaccacc atctcttctg
     1621 cgagctcttg ctctacgagc tcaagagcgt ccagcccggc atggagcccg atctacgcgc
     1681 cagggccagc gagtggctcg aggacgccgg tatgatcgag gaagccgtct ggcaggcttt
     1741 cgaggcggag aaccacgggc ttgcagggtt gcttatcgcg cgtcactggc gcgggtactt
     1801 gttcgccggc cagacggcga cggtgcagcg atggttgtct tcgttgccac aggatgttct
     1861 cgcccacagc gcgccgctta ccctggtgaa ggcctggata tgtgcccttg atggccagag
     1921 agaggagagt cagcggttcc tgcggctggc cgagagcttc gggtacgagg gcccacttcc
     1981 cgacgggatc gcctctgtcg agtcgggggt ggctttgctg gaggccacct tcggctacgg
     2041 tgggatccgc cacggggtcc agaccgcccg gcttgccgcg gagctggagc cgatagaaac
     2101 ctcgccatgg gccgtagtag catgcctcgc gctcggatcc agcctctatc tccagggaga
     2161 cctcccggag gcccgcaggg cgctggagaa tgccctctcg ctcgcagatg acaaccagcg
     2221 cctggtgcgg gtggtagcgc tgtcctttct gtctttcgtc gccacggacg aggggcacct
     2281 ggaggaagcc gagtcgctgg cgctggcggc gcaggcgctc gtggagaggt cgaggccgta
     2341 ccggatcccc cagaccaccc tggcaccaat cgcctacggc cgcgtgctcg cagagaaggg
     2401 aaacctcgaa gaagcccagg aggagttgga aggtgccctc tcctcccgag gacggttgcc
     2461 gggcctgagt ccatggccaa accttatcgg gctgctggcg ctcgccccgg tactcgccgg
     2521 acgcggcgac cgggacgggg ccagggcggc actcgctgag gtcagggcca tcctggatgc
     2581 ccatcccgac gccgggatgc tgccggagct gctcgagcgt cagaaccgca aacttcgcgt
     2641 gcgcagggcg agggaaggac gcctggacgc ggagctcacg gagcgcgagc tggaggtgat
     2701 cgggctgctc gacggacggc tcaccaccag gcagatggca gaaagcctct tcgtcgcacc
     2761 aagcaccgta aggacccaga tcaaatccat ctaccgcaag ctgggggtgt cttcacgcgg
     2821 cgaggccgtg gcagtagccc gggagcaggg gctcgtctag acttatcccg tctccacaga
     2881 tcccctaggg gaaacggtcc ggtgggtgat gacgaacgac ccgtgcttgc actaggctcg
     2941 ggaactaaaa agctatgagg atcggtggaa acataggatg cgcaagggca cagggaggtt
     3001 ttaccgaatt gtcgtgcgta gcgagctcgg caacgtcttc gctgcggcct tcgaggggat
     3061 ggaggtggag gctagagacg gggaaacggt ccttacgggc aggatcaccg accagccgca
     3121 actctacgga atactggaac gcataaacgg gctggggctg gagctgctca gcgtgcaggc
     3181 cctgacggac gatgccaacc agagcagcat agtgaaccga cgaccgtagg aaagggagcc
     3241 atgagcctag ccgtcttgcc cctggccatc acgatgaacg cggggccaca gataatgtcg
     3301 gccctaatct tcgttactgc ctccaaaccg atcaagctat cggcctactt cctgaccggg
     3361 gtcgtgatcg ccgtgaccgc gggcgtcacc attaccttcg gcatcgccag cgtgttcggc
     3421 aacggcatct cgctcggcga ttcctcggat tccggatcgg tcggcaacat catccagtat
     3481 ctgctggtcg ggttgctcgt ggtcgcctcg atcaggagct acctgacccg caagacgagc
     3541 gaaccgccgc gctggctcgg cgccatgcag aacgcggagc ccaagcaggc gttcctggcc
     3601 gggttgctgc tgctttcggt cttcccctcc gacttcgtgg tcctgatgac ggtgggggtg
     3661 aacctcgtac agaaggacgc ctcgcttctg cacgccctcc ctttcgtggc agcaaccata
     3721 ctcatagccg cgcttccgat gctctcttac ctgctcttcc gcaggcgcgc cgaggtactc
     3781 atgcccaagt tgcgcgactg gatgaacacc aacagctggt tggtgaacat catcgtctac
     3841 atcgtgttca tcttgctaat cgtctcttga ccggcggcaa caaataaaag aggagggcaa
     3901 tggggttgca caggaaggtc gatggcgatg gtctcgacgg gcgttacctt tcggtaagcc
     3961 cgcttctcga cggaaaggtt accgtaccaa agcataggct gcctgatggg ccgatgctgc
     4021 ccgagatggc ctacgagatc atccatgacg agctcatgct cgacggcaac gcccgtctca
     4081 acctggcgac gttcgtgacg acgtggatgg agccgcaggc cgagaggctg atggccgagg
     4141 cccttgacaa gaacatgata gacaaggacg agtaccccca gactgccgag ctggagatgc
     4201 gctgcgtcaa catgctaagc cgcctgtgga actctccgag caccgaccaa gcgacggggt
     4261 gctcgaccac gggctccagc gaggccgcca tgctcggggg gctcgccctc aagcgccgct
     4321 ggcagcagaa gaggcgtgcg gccggagaaa gcaccgagcg gcccaacatg gtcatgggag
     4381 taaacgtcca gatctgctgg gagaagttct gcaactactt cgaggtcgag ccccgctacg
     4441 tgccgatgga gggggatcgc ttccacctga acggcgagga ggcggtggcg ctctgcgacg
     4501 agaacactat cggcgtggtg gccgtactcg gctccacctt cgacggctcc tacgaaccgg
     4561 tggcggagat ctcggatgcg ctcgacgatc tggaggaccg tacaggcctc gacgtgccga
     4621 tccacgtgga cgcggcttcg ggggggatgg tcgcgccgtt tatcgacccc gacctcgtct
     4681 gggacttccg cctgccgcgc gtggcgtcga tcaacacctc gggtcacaag tacggtaggg
     4741 tgtaccccgg cgtcggctgg gccatatggc gtgatgcgga ggcgcttccg gaagatctcg
     4801 tgttccgggt aaactacctc ggcggagata tgctcacctt cgcgctcaac ttctcacggc
     4861 cgggttccca gatcgtggct cagtactaca acttcatcag gctcggtttc gagggctaca
     4921 ggcgcgtcca gcaggagtgc agggacatag caaccctcgg ttcggccgcc atcgccgagc
     4981 tcggcccctt cgagctcata accgacggct cggagcttcc cgtcttcgcg ttcaaggtgc
     5041 gcgacgacgt gagcgcgttc tccgccttcg atgtctcggg cggcatgcgc gaacgcggct
     5101 ggcaggtgcc agcctactcg ttccccgaga acagggagga cctcgtcgcg ctccgggtgg
     5161 tggtcagaaa tggttacagc agggaccccg cagatatgtt cctggacgac ctcaagcggt
     5221 tgctgacgcg cctggagaag caatcgtccc cgacgcacga cgaatcctac tccggtttcg
     5281 cccactgagg agacagggag ttcgcgagag gaagagaaaa ctctaccgtg caggcacgaa
     5341 ccgcacgtac agcgccatga caaccccaag gaggacctat tttgtcccag gatcgagagc
     5401 ttcgtaacga ggaccaggga gtaccggact acgccgccca gcgcggtgcc cgtctggagg
     5461 gcatcctggg acgggtcagc gacgcgccac tgcgccagaa caacggcatc tccctgctaa
     5521 agaacggtcc cgacacctac gatgattggc tcacggccat ctcgcgggcg aagcgttgga
     5581 tccacctgga caactacatc atcgcgaacg acgaggtcgg caaccggttc gccagggcca
     5641 tgtctgctaa ggccagggag ggtgtgcggg tgcgggtact gcacgactgg ttcggctgcc
     5701 tcgacgtgcc ccgctccttc tggcggggca tgcgggaggc cggtgttgag gtcaaggccg
     5761 taaacccgcc ggcctcggga cccccgctcg gtgccatccg gcgcgaccac cgcaagctac
     5821 tcgtcgtcga cggcgagtat gcctcaacgg gcggggtgtg catcgccgac ggctggctcg
     5881 tgagatcccc cgacacgggt ttgccctaca gggacaccgc cgtcagcgtg aggggaccga
     5941 tagtggccga cgtcaaccgt gccttcacca gcctgtggag cgagcaggta ggggaactac
     6001 ccgaggatga gagaccagat ccggagggga tcccgacggc gggagagacc gccgcgcgcc
     6061 tggtcgtgca ggaacccaga aggatgcgca ccctgaggat gctggagctc ctgacggccg
     6121 gggtgcagga gcgcctctgg ataaccgacc cctacttcct ctccatgccc atcctcaccc
     6181 agtcgctgat ggccacggcg cgcgacgggg tggacgtgag ggtcctcaac cccgcgaccg
     6241 tggacatagc gtggataggt agggcctccc gggccggata ccggcagttt ctggaggctg
     6301 gggtcaggat cttcgagtac ggagggccca tgatccacgc caagacgcta gtggccgacg
     6361 gctggctatc gaaggttggc tcgaccaacc
//
LOCUS       DBNGVD010000455         6403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_474679, whole genome shotgun sequence.
ACCESSION   DBNGVD010000455 DBNGVD010000000
VERSION     DBNGVD010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_474679"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="AC16CM_12250"
     CDS             <1..566
                     /locus_tag="AC16CM_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3140650.1"
                     /translation="ELPLGEFLDRVASDSPAPGGGSVAAVAVALAAGLAGMAARLSPE
                     RLADAPELAGRADAARMKVAPLARADAESYGRVLEAYREPDPEARSARAGDALSAAAD
                     VPLAVAEAGDEVVGIASRLAQEGNPNLEGDALAAVLLAGAGVRAAARLVEINLSAAGV
                     EDGRLRRANDLVDTTAAAVRRTTEGGR"
     gene            625..1923
                     /locus_tag="AC16CM_12255"
     CDS             625..1923
                     /locus_tag="AC16CM_12255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3140651.1"
                     /translation="MLERRTPFYDYHRRSARDLIQGGGSYMFPTSYASALEEHLNVRR
                     NVGMQDLSTMGEVDVKGPGAERLVRRLLVNEVQDMEPGQLRYSTMCNEEGGIVDDVTV
                     YKFDDEHFMITTSSGPREKAYRWISEHAVGSSAYVTDLTAGVALPVVQGPRSRSFLKT
                     VVEGVDLDELRFFRFSPCRIEDTELIISRSGYTGELGYELYTPADQASALWEYLLQQG
                     RSFNLKPYGLEAMQSLRIEKSLPLYGPDISEEETPFHVGLGRWVRFDKRDFVGREALL
                     RVQERGLDRRWVGLILESEVPATTGAGVYGVADVATFRDVVETGAEAGEQEDELMPGD
                     RQVGRVTSSAMGHTVGRMLAMAYLDTAHSWPGNNLVVELGGRPIPARVAQTPFFDPEF
                     ARVRARPHEDEQLSELVPKPARLTNGRVAASQSPNGNTRR"
     gene            1974..3173
                     /gene="solA"
                     /locus_tag="AC16CM_12260"
     CDS             1974..3173
                     /gene="solA"
                     /locus_tag="AC16CM_12260"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR3140652.1"
                     /translation="MGSATAYYLARRGKRVLGLEQFGIPHNMGSSHGHTRIIRLAYYE
                     DPSYVLLLRRAYELWREIQGKAGEKLLHVTGSVDAGPGESWVFKGSWESCKLHDLPHE
                     VLTGAELARRYPGYHLPPEHLALVQPEGGFLAPERCIVSYVMAAQSHGAEIHGHEQVL
                     EWTPLEGGVRVRTDRGTYEADRLVMTAGAWNGDLIDVLDGLAVPERQVLAWLQPTRPE
                     RFRPDNFPVFNLLVDEGRFYGFPVFGVPGFKFGKYFHLNETGRADEIDRQPHDHDEQL
                     LRSFAERYFPDGAGPTMDLQTCMFTNTPDHHFVIDVHPDYPQVSIASPCSGHGFKFAS
                     VVGEVMADLAEKGFTRHDISLFRLDRLRRRPGQRSDTPVRGGQPVKQTNGHHPSFERM
                     GDIRTFW"
     gene            3223..4011
                     /locus_tag="AC16CM_12265"
     CDS             3223..4011
                     /locus_tag="AC16CM_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3140653.1"
                     /translation="MGSALMCAVIRKSTSVQSVDRAITVMEFLARRGWSGVTEVSREL
                     DIHKSTAYRLLTTLRDRGLVEQDAATEKYRLGFGVVLLARAVSADLDVLRCAKPLCER
                     LSERTGETVTVAVLEGDDAVVIHQSLSRTSALSVDWTGHHTPLHATAAGKIFLSHMPE
                     DQRMRILEGPLERFTSNTTVDPETLREQVAQILDDGYSHTVEELETGLSAIGVPIRSS
                     EGTVVGAVSVSGPSFRMEAADLLSTVEMARRTTAEISRCLGYQG"
     gene            complement(4166..5452)
                     /locus_tag="AC16CM_12270"
     CDS             complement(4166..5452)
                     /locus_tag="AC16CM_12270"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor-dependent isomerase,
                     group 22"
                     /protein_id="HIR3140654.1"
                     /translation="MQTENPVYEEIREVLRPVELPSVALVGQRVDTPPALTSIREGVR
                     GALDAVELPSGSVAIGVGSRGVAKIGEIVAALVEVLKEAGAEPFVVPAMGSHGASTAE
                     GQADVLAHLGVSEESVGCPVRATMEATKIGETPSGVPVHVDRNAYEADSIVVVNRVKP
                     HTAFRGTVESGPTKMLAIGLGKQRGAHSIHSAGWEKIHETIPQAARVAVESGKVTFGL
                     ATIENADEEPCKIVAVPAERLEEAEAPLLEEAKRNLARLPFDDLDVLIVDEIGKNISG
                     DGADPNVTGRYPTTAASGGPSVDRMVYLGLTEETGGNANGLGMADVVTERLAGLMDRP
                     ATYMNALTSTTPAPVKTPMVMPTDEMAIAAALTMCAGVDPANARLVRIENTLKLGRIW
                     VSEALLGEVENDGRLEVLEDPRPMRFEEGEIRPSHA"
     gene            complement(5472..5873)
                     /locus_tag="AC16CM_12275"
     CDS             complement(5472..5873)
                     /locus_tag="AC16CM_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3140655.1"
                     /translation="MADGLCEGGCYCGRVRYRVSGEVLRSAICHCVSCRRTSGALTVA
                     WVSFPYRSFSFIEGEPADYRSSDEVSRTFCQSCGTSLTYRHDEDAGSIDVTTASLDQP
                     DEFPPTRHIWMEDRLVWEDVDDGLPRFEQGG"
     gene            complement(5943..>6403)
                     /locus_tag="AC16CM_12280"
     CDS             complement(5943..>6403)
                     /locus_tag="AC16CM_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140656.1"
                     /translation="VTSLHNLVQPIIRLELPDAVTIDPEPCQCGRTLVRTRAVEGRTD
                     DVLYLPARRGGEVMVHPLQFGVVTRDRGVREFQVIQEREGVRILVVASPAADDGLDRR
                     LRQAVSGKLAKLGAREPRVSVERRAELARSAGGKMQLVVADPGTRTAFHG"
BASE COUNT         1090 a         2152 c         2096 g         1065 t
ORIGIN      
        1 tggaactccc actgggagag ttcctcgatc gcgtggcctc ggatagcccc gctcccggtg
       61 ggggctccgt ggccgccgtt gccgtggcgc tcgccgctgg gctggccggc atggccgccc
      121 gcctctcccc ggagaggctc gccgacgccc cggaactggc cggccgggcg gatgccgcga
      181 ggatgaaggt cgcgccactt gccagggccg atgcggagtc ctacggacgt gtcctcgaag
      241 cctaccgcga gccggatccc gaggcccgtt cggcgcgtgc cggggacgcc ctctcggcgg
      301 ccgccgacgt gccgctggcc gtggccgagg cgggtgacga ggtggtcggc atcgcctccc
      361 ggctggcgca agagggcaac cccaacctcg aaggagatgc gctcgcggca gtcctgctgg
      421 ccggcgcggg ggtgcgcgcc gcggcgaggc tcgtcgagat caacctctcc gcggccggcg
      481 tggaggatgg taggctcagg agggcgaacg acctcgtcga tacgacagcg gcggccgtgc
      541 ggcgaaccac ggaaggcgga agatgaacag caaagatcct tgccgtataa tttacaacca
      601 gtacgtttga gggaggggca ggtcatgttg gagagaagga cgccgttcta cgactaccac
      661 cgacgttcgg ccagggatct gatccaaggt gggggaagct acatgtttcc gacctcctac
      721 gcctcggccc tggaggagca cctgaacgtg cgcaggaacg tggggatgca ggacctctcc
      781 acgatgggcg aggtggacgt gaaggggccc ggcgccgagc ggctggtgag gcgcctgctc
      841 gtaaacgagg tccaggacat ggagccgggc cagctcaggt actcgaccat gtgcaacgag
      901 gagggtggca tcgtagacga cgtcaccgtc tacaagtttg acgacgagca cttcatgatc
      961 accaccagct ccggtccacg tgagaaggca taccggtgga tctccgagca cgccgtcggc
     1021 tctagcgcct acgtgacgga cctgacggct ggcgtcgccc tgcccgtggt gcagggccca
     1081 cgctcgcgca gtttcctcaa gaccgtggtc gagggcgttg acctcgacga gctcaggttc
     1141 ttccggttct cgccatgccg catcgaggac acggagctca tcatctcgcg ctctggctac
     1201 acgggcgagc tcggctacga gctctacacg ccggcggacc aggcctccgc tttgtgggag
     1261 tacctgttgc agcaaggccg cagcttcaac ctgaaaccct acggcctcga ggccatgcag
     1321 tccctgcgca tagagaagag cctcccgctc tacggtccag acatcagcga ggaggagacc
     1381 cccttccacg ttggtctcgg acgctgggtc cggttcgaca agcgcgactt cgtcggccgg
     1441 gaggcgctgc tgcgggtgca ggagcgcggc ctggacaggc gctgggtcgg cctgatcctg
     1501 gagagcgagg ttcccgccac cacgggagcc ggggtctacg gcgtcgccga cgtggccacc
     1561 ttcagggatg tggttgagac gggggcagag gcgggcgagc aggaagacga gctcatgccc
     1621 ggcgataggc aggtcgggcg cgtaacttcg agcgccatgg ggcacaccgt gggcaggatg
     1681 ctggccatgg cctacctgga cacggcccat tcctggcccg gcaacaacct cgtcgtcgag
     1741 ctcggcggcc ggcccatccc cgccagggtg gcccagacgc cattcttcga tccggagttt
     1801 gcgcgcgtca gggcgaggcc gcacgaggac gagcagctct ccgaactggt ccccaagccg
     1861 gcgcggctca cgaacggcag ggtggcggcg agccagtccc ccaacgggaa caccagaagg
     1921 tgagcgtaca gtactccgga cgctacgacg caatcgtcgt gggtgtgggg gggatgggca
     1981 gcgccaccgc ctactacctg gcccgcaggg gcaagcgggt gctcggcctc gagcagttcg
     2041 ggataccgca caacatgggc tcctcacacg ggcacacgcg catcatccgg ctcgcctact
     2101 acgaggaccc atcctacgtg ctgctactca ggcgcgccta cgagctgtgg cgtgagatcc
     2161 agggcaaggc cggcgagaag ctgctgcacg taacgggctc tgtggacgcg ggcccgggcg
     2221 aatcctgggt cttcaagggc tcgtgggagt cctgcaagct ccacgacctc ccgcacgagg
     2281 tgctcaccgg cgccgagctc gcgcggcgct acccgggcta ccatcttccc cccgaacacc
     2341 tcgccctcgt ccagcccgag ggaggttttc tcgcgcccga gcggtgcatc gtgtcttacg
     2401 tgatggcggc gcagtcgcac ggcgccgaga tccacggcca cgagcaggtg ttggagtgga
     2461 cgccgctcga aggcggcgtg cgcgtccgca cagaccgcgg cacgtacgag gccgacaggc
     2521 tcgtgatgac cgccggggcg tggaacgggg acctcatcga tgttctcgac gggctcgccg
     2581 tgcccgagag gcaggtcctg gcgtggttgc agccgacgag gccggagcgc ttccgccccg
     2641 acaacttccc cgtcttcaac ctgctcgtgg acgagggccg cttctacggc ttccccgtct
     2701 tcggcgtgcc cggcttcaag ttcggcaaat acttccacct caatgagaca ggccgagccg
     2761 acgagatcga ccgccagccc cacgaccacg acgagcagct cctgaggagc ttcgccgaac
     2821 ggtatttccc cgacggcgca ggtccaacca tggacctgca gacctgcatg ttcaccaaca
     2881 ccccggacca ccacttcgtg atcgacgtgc accccgacta cccgcaggtc tccatagcct
     2941 caccgtgctc ggggcacggc ttcaagttcg ccagcgtggt gggagaggtc atggccgacc
     3001 tggctgagaa gggcttcacc cgccacgata taagcctctt ccggctcgac cggctgagaa
     3061 ggcgccccgg ccagagatcc gacacacccg taagaggtgg gcaaccggtg aagcagacca
     3121 acggccatca cccctccttc gaaaggatgg gggacataag gacgttctgg tagccgcggc
     3181 gtacgcccgg ccacagggcc gggccaggac atggaaggtg gggtgggatc cgcgcttatg
     3241 tgcgccgtaa tccgcaagag tacctcggta cagtcggtcg accgtgccat aaccgttatg
     3301 gagttcctcg cgcgccgcgg ttggtcgggc gtgaccgagg tctccagaga actggacatc
     3361 cacaaatcca ccgcctaccg gctgctcacg acgctgaggg accgggggct cgtcgagcag
     3421 gatgccgcga ccgaaaagta caggcttggc ttcggcgtgg tcctgctggc gagggccgtg
     3481 agcgccgacc tcgatgtcct gcgctgtgcc aaacccctgt gcgaacggtt gagcgagcgt
     3541 accggggaga ccgtgaccgt ggctgttctg gagggtgatg acgcggtcgt catccaccag
     3601 agcctctcgc gaacctccgc cctcagcgtg gactggaccg gccatcacac cccgttgcac
     3661 gccacggcgg cgggcaagat cttcctgtcc catatgccgg aggaccagcg catgcgtatc
     3721 ctggaaggac cgctggaacg ctttacaagc aacaccaccg tcgacccgga gacgctcagg
     3781 gagcaagtgg cccagatcct agacgacggt tactcccaca ccgtggagga gctggagacc
     3841 ggcctgagcg cgatcggggt gccgatccgc tcctcagagg gcaccgtggt tggcgcggta
     3901 agcgtgtcgg gaccctcctt ccgaatggag gccgcggacc ttctctccac cgtcgagatg
     3961 gccaggagaa ccacagccga gatctcccgg tgcctgggat accagggctg aaagctgtca
     4021 gccttcagcg gtcagctttc agccaaaaca acaacccgct agccaagcgt tcaatgctga
     4081 aggcgcgaac cacaacagga ccgcggacgc tcagccagtg tcctagatca acagctgaca
     4141 gctgatagct gaccgctaat agctatcaag catgactcgg cctgatctct ccttcttcga
     4201 agcgcattgg cctgggatct tcgagaacct ccagccgtcc gtcgttctcc acctcgccga
     4261 gcagggcctc ggagacccag attcttccca gcttgagcgt gttctcgatt cggacgaggc
     4321 gggcgttcgc cgggtctaca ccggcgcaca tggtgagggc ggcggctatg gccatctcat
     4381 ccgtgggcat gaccatgggc gtctttacgg gtgccggggt ggtcgaggtg agtgcgttca
     4441 tgtaggtggc gggcctgtcc atcaacccgg cgagtcgctc ggtgacgacg tcggccatac
     4501 cgaggccgtt ggcgttgccg cctgtctcct cggtcaggcc gaggtagacc atgcgatcta
     4561 cggatggccc gccggaggcg gctgtggtgg ggtagcggcc cgtcacgttc gggtcggcgc
     4621 cgtcgcccga gatgttcttc ccgatctcgt ccacgatgag aacgtctagg tcgtcgaacg
     4681 gaagccgcgc caggtttcgc ttggcctctt ccaggagcgg cgcctcggcc tcttccaacc
     4741 gttcggcggg gacggctact atcttgcacg gctcctcgtc ggcgttctcg atggtggcga
     4801 ggccgaaggt gaccttgccg ctttcgacgg cgacgcgggc cgcctgcggg atggtctcgt
     4861 ggattttctc ccaccccgcc gaatggatgg agtgcgcccc ccgctgcttg ccgagcccga
     4921 tagcgagcat cttggtcggg ccgctctcca ccgttccccg aaacgccgtg tgcggcttga
     4981 ccctgtttac gaccacgatg gaatcggcct cgtaggcgtt ccggtctaca tggaccggaa
     5041 cgccggaggg cgtctccccg atcttcgtgg cttccatggt cgcccgaacg gggcatccga
     5101 cgctctcctc gctcacgccg agatgcgcca gcacgtccgc ctggccttcc gccgtggacg
     5161 cgccgtggct gcccatcgcg ggcaccacga acggctccgc cccggcctcc ttcaagacct
     5221 ctaccagggc ggcgacgatc tccccgatct tcgctacgcc cctgctcccc acgcctatgg
     5281 ccacggagcc cgacggcaac tccacggcat ccagcgcccc gcggacgccc tcgcggatgc
     5341 tcgtgagggc tggtggggta tctacccgct gtccgacgag ggcgacgctc gggagttcga
     5401 ccgggcgaag tacctccctt atctcctcgt agaccgggtt ctccgtctgc accgcttcac
     5461 ctccgtggta atcaaccgcc ctgctcgaac cggggcagcc cgtcatcgac gtcttcccag
     5521 acgagtctgt cttccatcca gatgtggcgt gtgggaggga actcgtctgg ctggtccagg
     5581 ctcgcggtgg ttacgtcgat gctgcctgcg tcctcgtcgt gcctgtaggt gagcgacgtg
     5641 ccgcaactct ggcagaaggt tctgctcacc tcgtccgaag aacggtaatc ggccggctca
     5701 ccttcgataa acgaaaagct cctgtaggga aagctaaccc aggcgacggt gagagcgccc
     5761 gacgtcctcc ggcaagagac gcagtggcag atcgcggacc tcaagacctc tccggatacc
     5821 ctgtacctca ccctgccgca gtagcatcca ccctcgcaca gaccatcggc catcgcgtct
     5881 ccccctttca gcgaccattc attctaacag cctacgatgg tcgcccggcg cggtagaggt
     5941 actcagccgt ggaaagccgt tcgcgtgccc ggatcggcca ccaccagttg catcttgccg
     6001 cccgccgaac gcgcaagttc agcgcgacgt tccaccgaga cgcgcggttc tctggcgccg
     6061 agcttcgcga gcttgccgga cacggcctgg cgtagcctac ggtccaaccc gtcatccgca
     6121 gcagggctcg cgaccacgag tatgcgcacg ccctctcttt cctgtatcac ctgaaactcg
     6181 cgtacccccc ggtcgcgcgt gacgaccccg aactggagcg gatggaccat cacctctccc
     6241 ccgcggcggg ccggaaggta gagcacgtcg tcggtgcggc cttctacggc gcgggtacgg
     6301 acgagcgtgc gaccgcactg gcagggctcg gggtctatcg tgacggcgtc cggcaactcg
     6361 aggcggatga tcggctgcac caggttgtga aggctcgtca cca
//
LOCUS       DBNGVD010000456         4950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4845307, whole genome shotgun sequence.
ACCESSION   DBNGVD010000456 DBNGVD010000000
VERSION     DBNGVD010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4845307"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC16CM_12285"
     CDS             <1..794
                     /locus_tag="AC16CM_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3140657.1"
                     /translation="YDGIMKKLPTMYEQFLKLADIDISREPMEVFPTVHYTMGGIKIH
                     PETAASSVPGLFAAGEVAGGLHGANRLGGNSLSDLLVFGRRAGEGASAYIEESVHSAD
                     IDEGEVLGEIDRVLEPLEKKGEGESPYLIQQELQEAMMEHANLMRDGDSLKEGLGKIL
                     ALKDRLPQISVPGSRLFNPGWHTAQDVRYLVQISEIILRTALERKERRGAQWRLDFDG
                     PDEDLGKLNFIVKKDVQGEVGIERREIPPMPEHLASLVEEAKAKA"
     gene            881..1603
                     /locus_tag="AC16CM_12290"
     CDS             881..1603
                     /locus_tag="AC16CM_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3140658.1"
                     /translation="MAKLKIFRGDAEGGEHVDYEIPLVEGMVVLDAVLAIQAQQAPDL
                     AVRWNCKAAHCGSCSAEINGRPKLLCKTRVEEYDGAEIRVGPMRAFPVIKDLVSDVSW
                     NYQVSKGITPFTTSEQAPFTMYEEDVERLYEPKRCIECFMCQDVCHVLRTHHKHAQFA
                     GPRFFVRNQWLEMHPMDEADRLPDLKEENGLGFCNITKCCTEVCPVDIHITDNSIIPL
                     KERVADEYYDPAKWLMRKIRGR"
     gene            complement(1888..1960)
                     /locus_tag="AC16CM_12295"
     tRNA            complement(1888..1960)
                     /locus_tag="AC16CM_12295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1925..1927),aa:Gly,seq:ccc)
     gene            2014..2565
                     /locus_tag="AC16CM_12300"
     CDS             2014..2565
                     /locus_tag="AC16CM_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3140659.1"
                     /translation="MEQSYDSSEYNFCPRCAGRFEERVLKEGEPGRLVCESCGFIFYL
                     GPKLVAGALFEMDGGVVLVQRDIEPGYGKWTFPGGFVERGERAEAAAEREVLEETGLT
                     VRVSSIIGLYSYEGQVPAIAVFRADVTGGEPTPLDETMDVKVFSRDALPWPELAFPST
                     EHALRDYLADGGGEDAGESSPPG"
     gene            complement(2562..3113)
                     /locus_tag="AC16CM_12305"
     CDS             complement(2562..3113)
                     /locus_tag="AC16CM_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3140660.1"
                     /translation="MARVVVGVTGGIAAYKAPGVIRRLRESGHEVGVIATDAAFRFVP
                     EETLSIAAGGPIHTDRTWWEHSGRVEHVSLARWADLLLVAPATADAMARSAIGLGDDL
                     LSATILAGARAVLWAPAMNPEMWTNPSTVRNVETLKGWGHHFVGPADGLMASTEEAPG
                     VGRLAEETEIVAAVAAVLSEGDV"
     gene            3200..4471
                     /locus_tag="AC16CM_12310"
     CDS             3200..4471
                     /locus_tag="AC16CM_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR3140661.1"
                     /translation="MKSSGADRRELAAYALVFTGAFALAVGLFARLYRLGYPSRLVWD
                     EVYFPVFARAYLNGTPFFDLHPPLGKFVIAAGIALVGDSPVGWRIMPALFGCAMIPLA
                     AALGWYLFREWVAAVLLATTIACETMLVVYCRTSLMDGILVFFLLATFLSAVMARHRW
                     QVLLTMILLGLAVTVKWAALPVALPVGYILWRKGLLKPLLWTLWVPAVVYLCVVFVGQ
                     AVNPTGAGRDFNDNGTLTNVVNWHRQALVNVSRAVPNEQASPWWSWPLMTRPVLLFYQ
                     NQGTGRVTTVFAVGNPLVWWASTGAVLAGVGELLWRRLARGMPIADHPLVPIVLGFVC
                     LMLPWLPGTRIPYLYNYLPSYLFALLALVYWLCRLWGGRRWGPWVVVGFAAGAVSLAL
                     YFLPLAMGLPIGYENLQQHIWFESWDHDLFR"
     gene            4636..>4950
                     /locus_tag="AC16CM_12315"
     CDS             4636..>4950
                     /locus_tag="AC16CM_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140662.1"
                     /translation="MPRVSSPGWAGLALVPLLAVLSLPPLLWFGPHWTVYGNDSGRYL
                     LAGHQFITGETLQDLNTASEYNGGHGPGLPAMIGTLILLLGRDTIAIVWVLRLISLLN
                     PLL"
BASE COUNT          850 a         1595 c         1633 g          872 t
ORIGIN      
        1 ggtacgacgg gatcatgaag aagctcccga ccatgtacga gcagttcctc aagctcgccg
       61 acatagacat ctccagggag cccatggagg tcttccccac ggtccactac accatgggtg
      121 gcatcaagat ccaccccgag accgcagcct ccagcgtgcc tggtctcttc gccgccgggg
      181 aggtagccgg cgggctgcac ggcgccaacc gcctgggtgg taactccctc tccgacctgc
      241 tcgtcttcgg aaggcgtgcc ggagagggag cctccgcgta catcgaggag agcgtgcatt
      301 cagcggacat cgacgaggga gaggtgctag gtgagatcga ccgcgtcctc gagccgctgg
      361 agaagaaggg ggagggtgag agcccgtacc tgatccagca ggagctccag gaggccatga
      421 tggagcacgc caacctcatg cgcgacgggg attccctgaa agagggcctc ggcaagatac
      481 tcgcgctcaa ggaccggctg ccgcagataa gcgtgccagg ctccaggctc ttcaacccag
      541 gatggcacac ggcccaggac gtacgttact tggtccagat ctccgagatc atcctcagga
      601 ccgcgctgga gcgcaaggag cgccgggggg cgcagtggcg cctggacttc gacggccccg
      661 acgaggatct cgggaagctc aactttatag tgaagaagga cgtgcagggc gaggttggca
      721 tcgagcggcg tgagatcccg ccgatgccag agcacctggc gtctcttgtg gaggaggcga
      781 aggcgaaggc atgagcgaag gaaacggcaa caacccgggg ggagtgacct ccaaggccca
      841 ctccccagta ccgaacgagg atctgggcaa ggtcgacagg atggcgaagc tcaagatctt
      901 ccgcggcgac gcggagggcg gcgagcacgt cgattacgag ataccgcttg tggagggcat
      961 ggtcgtcctc gacgcggtgc tggcaatcca ggcccagcag gcccccgacc tggcggtacg
     1021 ctggaactgc aaggccgccc actgcggatc gtgcagcgct gagatcaacg gtcggcccaa
     1081 gctcctgtgc aagacgcgcg tggaggagta cgacggggcg gagatccggg tcggccccat
     1141 gcgggccttc cccgtgataa aggacctggt ctccgacgtg tcgtggaact accaggtgtc
     1201 caagggcatc acgccgttca ccaccagcga gcaggcgcct ttcacgatgt acgaggagga
     1261 tgtggagcgc ctctacgagc cgaagcgctg catcgagtgc tttatgtgcc aggacgtctg
     1321 ccacgtcctg cgcacgcacc acaagcacgc ccagttcgcg ggccccaggt tcttcgtcag
     1381 gaaccagtgg ctcgagatgc atcccatgga tgaggccgac aggctgcccg acctgaagga
     1441 ggagaacggg ctcggcttct gcaacatcac caagtgctgc acagaggtct gcccggtaga
     1501 catccacatt acggacaact ccatcatccc cctcaaagag cgcgtagccg acgagtacta
     1561 cgacccggcc aagtggctca tgcgcaagat aaggggaaga tagcttgtca gcacgctcgg
     1621 gctagcgccc tcgcttgtcg gcaacgcaac gcccggcttc gccaggctcg ctttcggcag
     1681 gtcgtctcgg tccgtaacgg cagcttcggc atggggcttc cccgcggcgc taccttctgt
     1741 gcgtgaggtg caggccgcag cgggatctag ccgaaagcga agccctgtaa gggctgaagc
     1801 gttgccgaca agcgaccgca gggagcgtgc tgacaagctc gcgaagcggg cgtgcgcacg
     1861 cggaggcgaa agccggagcg tgctaagagc ggacgacggg cttcgaaccc gcgacctcca
     1921 gcttgggaag ctggcgctct accaactgag ctacgtccgc gtgaggtagg gattttagca
     1981 cgccgggcac ggctctagta acctggaaag actttggaac agagctacga ttcttcggag
     2041 tacaatttct gtccccgctg cgcgggcaga ttcgaggaac gcgttctcaa ggagggtgag
     2101 cccgggcggc tggtctgcga gagctgcggg ttcatcttct acctgggacc caagctggtg
     2161 gccggggctc tcttcgagat ggatggtggg gtcgttctcg tccagcggga catcgagccc
     2221 ggctacggca agtggacctt ccccggcggt ttcgtggagc gcggcgagcg ggcggaggcc
     2281 gccgcggagc gtgaggtgct ggaagagacg ggccttacgg tcagggtctc gagcatcatc
     2341 gggctctatt cctacgaggg tcaggtcccg gccatcgccg tcttccgggc ggacgtgacc
     2401 ggcggagagc ccacaccgct cgatgagacg atggatgtga aggtattctc gcgcgacgcc
     2461 ctaccgtggc ccgagcttgc cttcccgagc accgagcacg cgctgaggga ctacctggcc
     2521 gacggtgggg gagaggacgc gggcgaaagc agcccgccgg gctagacgtc accttcggac
     2581 aacaccgcgg cgacggccgc gacgatctct gtctcctcgg ccagccgtcc gaccccgggc
     2641 gcctcctccg tggaagccat gagcccatct gcggggccga cgaagtggtg tccccacccc
     2701 ttcagcgtct cgacgttgcg tacggtcgac gggttggtcc acatctccgg gttcatcgcc
     2761 ggggcccaga gcaccgctcg ggcgccggcc aggatcgtgg ccgagagcag gtcgtcgccg
     2821 aggccgatgg ccgaacgcgc catcgcatcg gcggtggcag gggccacgag gaggagatcc
     2881 gcccagcgtg ccagcgacac gtgctcgacc cgcccggagt gttcccacca ggtcctgtcc
     2941 gtgtggatgg ggccgccggc tgcgatggag agcgtctcct ccgggacgaa gcggaaggcc
     3001 gcgtcggttg cgatcacgcc gacctcgtgc ccggattccc ttaggcgacg gatcacccca
     3061 ggtgccttgt acgccgcgat gccgcctgta acgcccacaa ccaccctggc catgccccca
     3121 gtatacagag cgttcccgag gtcaggccga aggagactcc gtcgtgccgg gacgattgcg
     3181 agctagtacc atgcgccgtg tgaagagttc aggtgccgac agacgggagt tggccgccta
     3241 cgcgctggtc tttaccggcg ccttcgcgct ggcggttggc ctgtttgcca ggctgtaccg
     3301 gcttggctat ccatcgcggc tcgtgtggga tgaggtctac ttccccgtct tcgcccgggc
     3361 ctatctgaac gggaccccat tcttcgacct gcaccctccc ctgggcaaat tcgtcatcgc
     3421 cgccggcatc gccctcgtgg gcgactcgcc ggtagggtgg aggatcatgc cggctctgtt
     3481 cggatgcgcc atgatcccgc tcgcggccgc gctcggctgg tacttgttca gggagtgggt
     3541 cgctgccgtg ctgcttgcca ccacaatcgc ctgcgagacg atgcttgtcg tctactgccg
     3601 tacgagcctc atggacggta tcctcgtgtt tttcttgctc gcgaccttcc tctcggccgt
     3661 gatggcacga catcggtggc aggtcctgtt gacgatgata ctgctcggtc tcgccgtgac
     3721 cgtgaagtgg gcggccttgc ccgtggcctt gcccgtaggc tacatcctct ggcgcaaggg
     3781 cttactgaaa cccttgctgt ggacactctg ggtccccgcg gtcgtctacc tctgcgtggt
     3841 gttcgtcggg caggctgtca acccgacagg ggccggtaga gatttcaacg acaacggcac
     3901 cctcacaaac gttgtcaact ggcaccgtca ggccctggtc aacgtctcca gggccgtccc
     3961 gaacgaacag gcctcgccgt ggtggagctg gcccctgatg acccgccccg tgctgctctt
     4021 ctaccagaac caggggaccg gcagggtgac caccgtgttc gccgtcggca acccgctcgt
     4081 ctggtgggcg agcacgggcg ccgtcctcgc cggcgtcggc gagttgctgt ggcgtcgtct
     4141 ggcacgtgga atgccaatcg ccgaccaccc gctcgtgcca atagtcctcg gcttcgtatg
     4201 tctcatgcta ccgtggctgc ccggcaccag gatcccctac ctctacaact acctgccgtc
     4261 gtatcttttc gccctgctgg ccctggtcta ctggctctgc cggctctggg ggggccgacg
     4321 ctgggggccc tgggtagtgg ttggcttcgc cgccggcgcc gtatccctgg ccctgtactt
     4381 tctgcccctt gcgatgggtc tgccgatagg ctacgagaac ctgcagcagc acatctggtt
     4441 cgagtcctgg gatcacgacc tgttccgatg agcggcttcc gcggaggagg tcgctgcttg
     4501 ctcacctgcg gacggttcgg tacgcgccag gcacgctgta aactggggcc acgaacctgg
     4561 gtcgaggact ccggccgccg aacgtagagg ggagagattc aaatacaggg taggaacggg
     4621 tctctgagat cccggttgcc gagggtttcc agccccggct gggcggggct ggcgctcgtc
     4681 ccactcctcg cggtcctgtc cttgccgccg ctgctctggt tcgggcccca ctggaccgtc
     4741 tacggtaacg atagcgggcg ctatctgctg gccggccacc agttcatcac cggcgaaaca
     4801 ctacaggatc tgaacaccgc ctcggaatac aacggtggcc acggcccagg cctgcccgcc
     4861 atgatcggca ccctcatact cctgctcggc cgcgatacga ttgcgatcgt ctgggtcctg
     4921 cgcctgatct cgctcctcaa cccgcttctg
//
LOCUS       DBNGVD010000457         2933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2841686, whole genome shotgun sequence.
ACCESSION   DBNGVD010000457 DBNGVD010000000
VERSION     DBNGVD010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2841686"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="AC16CM_12320"
     CDS             <1..735
                     /locus_tag="AC16CM_12320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140663.1"
                     /translation="CETRVGSLPSGGERALLVREMLDRIAGRYDLLNTEMSAGLHRLW
                     NRRVVDAACLNPGGTALDLACGTGSLTRDLARKAGPEGHVLGIDFSREMLRAARSRTT
                     PGIAYRLGDATDLEGVPSDSFDAATIAYGARNIPDLDALFAGMARVLKPGGVAVCLEI
                     ARPTGGVSSRFYGLWFDRIVPKLGGLISGDASAYSYLPESVKEFVAPDELADIIGRNG
                     LQDVTWRRLAGGIITLHRGIKRPGDG"
     gene            707..1699
                     /locus_tag="AC16CM_12325"
     CDS             707..1699
                     /locus_tag="AC16CM_12325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3140664.1"
                     /translation="MGSNAPGTADGLDSEVLEGAFETVDEVEGMLARVAEGAPQPLIE
                     PALVALTSGGKRLRPLLLVLSARMGGPERGELLQAATAIEVLHTATLIHDDIVDGARS
                     RRGVPTTVEEHGREIAAATGDFLFAEAFARLAGIGDARLVRAFAVASVGLASGELEQY
                     RANGANVEVEAYLEHIRKKTAGLFQVACVAGATLGGLSLVQIDALASYGQALGIAFQM
                     SDDIMDLVGKPGLMGKGIGTDLGEGTVTLPVIFALKEGDAATIRRVLADPSPSPELLE
                     AGIEAVLATGAIARTEAWARGEIDAALRDLSLLPDSPEREFLRSVAAEVVGRDA"
     gene            1692..2933
                     /locus_tag="AC16CM_12330"
     CDS             1692..2933
                     /locus_tag="AC16CM_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3140665.1"
                     /translation="MPEGQPVIFAAGTLMPVSSPPVGDGAVLVEDGRISSVGPLSEVG
                     RENPNVEIRFFPRYALIPGAVNAHAHLGFRREDKPEGGTFAGWLSKLIERLPEKESWG
                     AEAARNSAREAVEAGTTYMAESSPYGECLPQLAASGMAGTVFAEFFPHEIGTPEEAVE
                     YIVDKADGLRKGLPERVGISVSVHAPYTVDPESSRLAAQKTRDLGWGLAIHLSESPEE
                     VEFVRHGTGGLADIFGSNEWGGVGVSPVRYADDVGLLAPQTIAAHLATGVSEEEIDIL
                     AQSGVAAAHCPRSNDYLGCGVSPVPRMVERGIRVGMGTDGLWSSPSMNLFEETMFAVD
                     LHGLDGATGLELATLGGARALGIASETGSLEPGKWADFALVELALGDQGSEREVLEAA
                     AGGAVALTVVGGDVIYNRLGA"
BASE COUNT          477 a          916 c         1053 g          487 t
ORIGIN      
        1 tgcgagacca gggtgggtag tttgccatcc gggggagaaa gggcgctcct ggttcgcgag
       61 atgttagaca ggatcgccgg ccgctacgac ctgcttaaca ccgagatgtc ggcggggctc
      121 caccgtctct ggaatcgcag ggtggttgac gccgcttgcc tcaacccagg tggcacggcg
      181 ctcgatctgg cctgcggcac gggtagtctt acgcgcgacc tggccagaaa ggcggggccc
      241 gagggccacg tgctaggcat agacttctcc cgggagatgc tgcgtgccgc gcggtcgagg
      301 acgacgcccg gcatcgccta caggctaggg gacgccaccg acctcgaagg cgtgccctcc
      361 gactctttcg acgcggccac catcgcctac ggggcacgga atattcccga cctcgatgcg
      421 ctgttcgcag ggatggcgcg ggtgctcaag cccggtggcg tcgccgtctg cctcgagata
      481 gccaggccca cgggtggggt gtcctcccgt ttctacgggc tctggtttga caggatcgtg
      541 ccaaagctcg gaggtttgat ctccggagac gcctcagcgt actcttatct gcctgaaagc
      601 gtgaaagagt tcgtggcgcc ggacgaatta gctgacataa tagggcgtaa tggattacaa
      661 gacgttacat ggagacggct cgcgggtggc atcatcacgc tccatcgtgg gatcaaacgc
      721 cccggggacg gctgacgggc tcgactcgga ggtgctagag ggggcgttcg agaccgtcga
      781 tgaggtcgag ggcatgcttg cccgggtggc cgaaggcgcg ccgcagccct tgatcgagcc
      841 tgcgctggtc gcgcttacct cggggggcaa gcggctaagg ccgctgctcc tggtgctgag
      901 cgcgcgcatg ggcgggccgg agcgggggga acttctccag gcagcaaccg ccatcgaggt
      961 gttgcacacg gcgaccctga tccacgacga tattgtggac ggggcacgga gcaggcgtgg
     1021 cgtgccaacg accgtcgagg agcacgggcg cgagatcgcc gccgccacgg gtgactttct
     1081 cttcgccgag gctttcgcca ggcttgccgg tatcggggac gccaggctcg tgcgcgcttt
     1141 cgccgtggcc tccgtggggc tcgcttcggg tgagctcgag cagtaccgcg cgaacggtgc
     1201 caacgtggag gtcgaggcgt acctcgagca catcaggaag aagaccgccg gcctcttcca
     1261 ggtcgcctgc gtggccggcg ctacactggg cgggctctcg ctcgtgcaga tagacgccct
     1321 ggccagctac ggccaggccc tcggtatcgc cttccagatg tccgatgaca taatggacct
     1381 ggtgggcaaa ccgggactca tgggcaaggg catcggcacg gacctgggtg agggcaccgt
     1441 gacgctgccc gtcatcttcg cgctcaagga gggggatgct gcgacaatcc gccgcgtcct
     1501 cgccgatccg agcccttctc ccgagctgct cgaggccggc atcgaggccg tgctcgccac
     1561 cggcgccatc gccaggaccg aggcctgggc gcggggtgag atcgacgcgg ccctcaggga
     1621 cctctccctc ttgcccgact ccccggagcg cgagttcctc aggtcggtgg ccgcggaggt
     1681 ggtcgggagg gatgcctgag ggccagcccg taattttcgc ggccggaacc ctcatgccag
     1741 tctcttcgcc gcccgtgggt gacggcgccg tgctcgtgga ggatggccgc ataagctccg
     1801 tgggaccgct ctccgaggtc gggcgcgaaa acccgaacgt cgagatacgc ttctttcccc
     1861 gctacgccct gattcccgga gcggtcaacg cccacgccca cctcggcttc aggcgggagg
     1921 acaaaccaga gggcggcacg ttcgcgggct ggctgagcaa gctgatagaa cgcctcccag
     1981 aaaaggagag ttggggcgcc gaggccgccc gcaactcggc cagagaggcg gttgaagcgg
     2041 ggaccaccta catggccgag tcctcgcctt acggggaatg cctgccgcaa ctagccgcga
     2101 gcggcatggc cgggaccgtc tttgccgagt tctttccgca cgagatcggc accccggaag
     2161 aggcggtgga gtacatcgtg gacaaggcgg acgggcttcg caagggcctg cccgagcgcg
     2221 ttggcatctc ggtcagcgtg cacgctccgt ataccgtcga tcccgaatcc tcgcgccttg
     2281 cggcgcaaaa gacgcgggac ctgggatggg ggctggcgat acacctttcc gagagccccg
     2341 aggaggtgga gttcgtgagg cacggcacgg gtggcctcgc cgacatcttc gggagcaacg
     2401 agtggggagg ggttggggta tctcccgtcc gttacgcgga cgacgtggga cttctcgccc
     2461 cgcagaccat cgcggcccac ctcgccaccg gcgtctcgga agaggagata gatatcctgg
     2521 cgcagagcgg cgttgcggcg gcccattgtc cgcgctccaa cgattacctc ggctgcggcg
     2581 tctcgccggt gccgcgcatg gtcgagcggg gcataagggt cgggatgggc acagacgggc
     2641 tctggagcag cccgagcatg aacctcttcg aggagacgat gtttgcagtt gatctgcacg
     2701 gtctcgacgg cgcaaccggc ctggagcttg cgacgctcgg cggggcgcgt gccctcggca
     2761 tagcgagcga gacggggagc ctggaacccg gcaagtgggc cgatttcgcg ctggtcgagc
     2821 tcgcgctcgg tgaccagggt tcggagcggg aggttttgga ggccgcggcg ggtggggccg
     2881 tcgccctcac ggtcgtcggg ggcgacgtga tatacaatcg tctcggtgcc tga
//
LOCUS       DBNGVD010000458         4584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1385822, whole genome shotgun sequence.
ACCESSION   DBNGVD010000458 DBNGVD010000000
VERSION     DBNGVD010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1385822"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16CM_12335"
     CDS             <1..615
                     /locus_tag="AC16CM_12335"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3140666.1"
                     /translation="RVGEGETSEQGLRQLGRTVGYVFQNPDHQIFSDTVGEEVAFGPK
                     IRGLDEGEIQERVEEALAAVGLEGRGGEDPFGLTKGERQRVAVASVLAVRPEVLILDE
                     PTTGLDYAEQRSMMDLVRSLNEAGSTIIVVTHTMWVVAEYAHRAVVVREGEISLRGTV
                     REVFAEEDDLREAYVRPPHIVTLGNMLGTTVLSVEEMMAVTEEG"
     gene            615..1394
                     /locus_tag="AC16CM_12340"
     CDS             615..1394
                     /locus_tag="AC16CM_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3140667.1"
                     /translation="MDPFLYLDRDTWVHRLDPRTKMLLLVGTFVLAFLFVNPLYGLAV
                     LTVVLLFGAAARSLANLKRVRFILVMIGLVTVVLWSVFAQGETHLFLFVEWESLLYGL
                     GVALRIDATIVAGMIFLSTTRNEEIATGLVRLGLPYRFAFAVSTALRLVPTIAATGAT
                     ISQAQRSRGLDLESGNIFQRVRKNTPLLIPVFVSTIRSTNVFSMALESKGFGASAQRT
                     YLLDLAMTRRDWLVLAAFVLLLAAAGALRLLGLGGLEGVTR"
     gene            1524..2378
                     /locus_tag="AC16CM_12345"
     CDS             1524..2378
                     /locus_tag="AC16CM_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR3140668.1"
                     /translation="MKEIFTMWRHTQMVVLVALSAAIYAAVLIPFKGFTIVPGFTEVR
                     PANVFPFVFGLLFGPAGAWGAAIGNLIGDFFGTLGIGSVAGFIGNFFLGFLPYKMWHT
                     FFRRGENMEPNVNSGKKLAVYAAVVVLASAVCAVWIAWFNDLIGFVPFAALGSIITVN
                     NAIAGLVLGPILLLVLYPRVKRWGLLWTEIMLPEEVPAARFGLLANALVWIGGLGGLI
                     VGLILGLGLFGQGLAAAGFATEAAGVTLGLILIPFLALVVVGSAMLGGREQVEATEQL
                     MEHEGSEV"
     gene            2388..3650
                     /locus_tag="AC16CM_12350"
     CDS             2388..3650
                     /locus_tag="AC16CM_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3140669.1"
                     /translation="MQDSVVKDPALAPDGHRKIDWAAEHSPVLNTVRDNFLNDGTFDG
                     LGVGVCLPIEAKTAYLTVVLAEAGADVAVASPGPFFVQDDVAAALAERGVTVYASSES
                     DPEDAELELERVIDRVTDGRDAVLIDDRAGLARLAHITRAGRLDRIRGVSEETTSGVA
                     KFRAMEREGVLEFPAIAANDARCKYLFDNRYGTGQSTLTAIMQNTNLMIGGKRLVVLG
                     YGWCGKGIARYAAGLGARVSVCEVDPVRGLEAYADGFDVLPALEAAEVGEIFITATGS
                     SGVLTEKHFERMRDGAILANAGAVDVEIDVDGLRKAARDTREVRRNVEEFVAADGRRL
                     YLVGRGMVVNLTAGDGHPIEIMDLTFAIQALCAYRLANEHASMENRVHLLPAEIDDEV
                     ARTKLATVGMSVDALTPEQEQFLTAWRD"
     gene            complement(3717..4232)
                     /locus_tag="AC16CM_12355"
     CDS             complement(3717..4232)
                     /locus_tag="AC16CM_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140670.1"
                     /translation="MLTRARFILYLASSTALLVAGVFLLWSPGAGGISAVEPPELRSA
                     PEPYVAVGRDYTTPDPPDVSIGSFRRPDDVPDYRILETYKTERDGARGATLTVDTRAT
                     TESDLTLIARDLKVRYADLDSVSVEFIDSEDFLNYRGGALIFNTAAGAYFQGFVYGPP
                     NNEGYLVNAAD"
     gene            4340..>4584
                     /locus_tag="AC16CM_12360"
     CDS             4340..>4584
                     /locus_tag="AC16CM_12360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3140671.1"
                     /translation="MARRTYDVVIVGSGPAGYTAALYASRANMKVLVFQGFESGGQLM
                     LTSDVENYPGYRDGVMGPEMMDDFEAQAARFGAEMRP"
BASE COUNT          775 a         1451 c         1551 g          807 t
ORIGIN      
        1 agggtggggg agggcgagac ttcggagcag gggctgcgcc agcttggccg gaccgtcggg
       61 tacgtgttcc agaaccccga ccaccagatc ttctcggaca ccgtcggcga ggaggtggcc
      121 ttcggaccaa agatccgggg cctcgatgag ggcgagatcc aggagcgggt ggaagaggcg
      181 ctcgccgccg ttgggctgga gggccgcggc ggggaggacc cgttcgggct caccaagggc
      241 gagcgccagc gcgtcgccgt ggcgagcgtg ctcgccgtgc gccccgaggt tctgatcctg
      301 gacgagccga ccacgggcct cgactacgcc gagcaacgca gcatgatgga tctggtcagg
      361 agcctgaacg aggctggctc cacgatcatt gtggtcaccc acaccatgtg ggtcgttgcc
      421 gagtacgccc acagggccgt cgtcgtgagg gagggtgaga tctccctccg gggaacggtc
      481 agggaggtct tcgccgagga ggacgacctc agggaagcct acgtcaggcc gcctcatata
      541 gtcacccttg gcaacatgct tggcaccacg gtcctctccg tagaagagat gatggccgtg
      601 acggaggagg ggtagtggac cctttcctct acctcgaccg agacacctgg gtccaccgtc
      661 tggacccgag aaccaagatg ttgctgctcg tcgggacctt cgtgctcgcg ttccttttcg
      721 tcaacccgct ctacgggcta gcggtcctga cggtcgtcct gctcttcggc gcggcggcga
      781 gatcactcgc caacctcaag cgcgtgaggt tcatcctcgt catgatcggg ctcgtcacgg
      841 tagtgctgtg gtccgtcttc gcccagggag agacgcacct cttcctgttc gtcgagtggg
      901 agtcccttct gtacgggctt ggggtcgccc ttcggataga cgccaccatc gtggccggca
      961 tgatcttcct ctcgaccacg cgcaacgagg agatagccac tggcctcgtc cgcctgggcc
     1021 ttccctaccg cttcgccttc gccgtttcca cggcccttcg gctcgtgcca accatagcgg
     1081 ccacgggcgc gaccataagc caggcccagc gctccagggg cctggatctc gagtcaggca
     1141 acatcttcca gcgggtccgc aagaacacgc cgctcctgat acccgtcttc gtatcgacca
     1201 tccggtcgac gaacgtgttc agcatggccc tcgagtcgaa gggcttcggc gcgagcgccc
     1261 agcgcaccta tctcctggac ctcgcgatga cgcgccggga ctggcttgta cttgcggcct
     1321 tcgtgctact gctggcggcc gcgggcgccc tgcgcctcct tggcctcggc ggcctcgaag
     1381 gcgtaaccag gtgatggaag gagccggggg aagatgacgg cgtctggtag gcggctggat
     1441 tatctgtcgc gccacaaggg tattggcaag aaggtcgggg ttggaagccc cggagcaggt
     1501 tccgtacgat cgcagggagg accatgaaag agatctttac gatgtggcgg catactcaga
     1561 tggtggtgct cgtggccctg agcgccgcga tctacgcggc cgtcctgata ccgttcaagg
     1621 ggttcaccat agtgcccggg ttcacggagg tacgcccggc caacgtgttc ccgttcgtct
     1681 tcgggctcct cttcgggccg gcgggcgcgt ggggggccgc catcgggaac ctcataggcg
     1741 acttcttcgg gacccttggc atcggtagcg tcgccgggtt catcggcaac ttcttcctcg
     1801 gctttcttcc ttacaagatg tggcacacgt tcttccggcg cggggagaac atggagccaa
     1861 acgtcaactc cggcaagaag ctggccgtct acgcggccgt ggtcgtgctc gcctccgccg
     1921 tctgcgcggt ctggatcgcc tggttcaacg acctcatcgg cttcgtgcct ttcgcggccc
     1981 tgggctcgat catcacggtt aacaacgcta tagccgggct cgtgctcggc cccatcctgc
     2041 tgctggtact ctacccgcgc gtcaagcgct gggggctctt gtggacggag atcatgctcc
     2101 ccgaggaggt cccggccgcc cgctttggac tgcttgcaaa cgccctggtc tggatcggcg
     2161 gcctcggcgg cctcatcgtc ggcctcatcc tcgggctcgg cctgttcggc caggggctcg
     2221 ccgccgcggg cttcgccacc gaggccgccg gcgtgacgct tggcctgatc ctcatcccgt
     2281 tcctggccct cgtcgtcgtg ggcagcgcga tgctgggtgg ccgcgaacag gtcgaggcaa
     2341 ccgagcagct gatggagcac gaaggatcgg aggtttgagg taaagatatg caggattccg
     2401 tagtcaagga cccggcgctc gcccctgacg gccacagaaa gatcgactgg gccgccgagc
     2461 actcgccggt cctgaacacc gttcgcgaca acttcttgaa cgatggcacc ttcgatggcc
     2521 tcggcgttgg cgtctgcctg cccatcgagg cgaagacagc ctacctgacg gtcgtgctgg
     2581 ccgaggccgg ggcggatgtg gccgtcgcca gcccgggccc gttcttcgtg caggacgacg
     2641 tggccgcggc gctcgcagag cgtggcgtta ccgtctacgc ctcctccgag agcgaccccg
     2701 aagatgccga gctcgagctc gagcgcgtga tcgaccgggt gacggacggc cgggacgccg
     2761 ttctgatcga cgacagggcg ggcctggcgc gcctggccca catcacgcgg gcaggacggc
     2821 tggaccgcat caggggggtc tccgaggaga cgactagcgg ggttgccaag ttcagggcta
     2881 tggagcggga gggcgtactc gagttccccg cgatcgcggc caacgacgcg aggtgcaagt
     2941 acctcttcga caaccgctac ggcacgggcc agtcgacgct gacggcaatc atgcagaaca
     3001 ccaacctgat gatcgggggc aaacgcctcg tcgtgctcgg ctacggctgg tgcggcaagg
     3061 gcatcgcccg ctacgccgcc ggcctcggcg cccgcgtctc cgtgtgcgag gtcgacccag
     3121 tccgcggcct ggaggcctac gccgacggct tcgacgtact accagccctc gaggccgcgg
     3181 aagtggggga gatcttcatc acggccacag gcagctccgg cgtgctcacc gagaaacact
     3241 tcgagaggat gcgggacggg gccatcctgg ccaacgccgg cgcggtggac gtggagatag
     3301 acgtggacgg cctcaggaag gcggcaagag atacccgcga ggtccgtcgc aacgtcgagg
     3361 agttcgtcgc ggcagacggc cgcaggctct acctcgtggg gcgtggcatg gttgtcaacc
     3421 tgacggcggg cgacgggcac cccatcgaga tcatggacct caccttcgcc atccaggctc
     3481 tctgcgccta ccgcctcgca aacgagcacg cctctatgga gaaccgcgtg cacctgctcc
     3541 ccgcagagat agacgacgag gtcgcccgta ccaagctggc aaccgtgggc atgtccgtcg
     3601 acgccctgac ccctgaacaa gaacagttcc tgaccgcctg gcgagactga cggcgcaacg
     3661 cgccgtcacg actgtcagca actacaaaat agctgaaagc tgacggctaa cagctttcag
     3721 tccgccgcgt tgaccagata gccttcgtta ttgggtgggc cgtagacgaa gccctggaag
     3781 taggcgccgg cggcggtatt gaagatgagc gcgccgcccc tgtagttcag gaagtcttcg
     3841 gagtctatga actcgacgga gacggagtcg aggtcggcgt agcggacctt caggtcgcgg
     3901 gcgatgagcg tgaggtccga ctccgtggtg gccctggtgt caaccgtcag ggtcgcccct
     3961 ctggcgccat cgcgctcggt cttgtaggtc tccaggatcc ggtagtcggg cacgtcgtca
     4021 ggccgccgga agctcccgat gctgacgtcc ggagggtcgg gggttgtgta atccctcccc
     4081 acggccacgt aaggctccgg ggcggaacgc agctccgggg gctcgacggc ggatatcccc
     4141 ccggcacctg gggaccagag caagaagaca ccggccacca gcagcgccgt ggaggaggcg
     4201 aggtacagga tgaaccgggc cctggtgagc atgaggaaga gtttagcccc atgcgcgatg
     4261 taaagctcac accatacggc ttgcaagatg tattagacta tatgtgtatg agagttcgca
     4321 ttcgggagga gcgtttctga tggctcgaag aacgtatgat gtggtgatag tcggctcggg
     4381 tcccgcgggg tacacggccg ccctctacgc gtcaagggcg aacatgaagg tgctggtttt
     4441 ccagggtttc gagagcggcg ggcagctgat gcttacctcc gacgtggaga actacccggg
     4501 ctatcgcgac ggcgtgatgg gccccgagat gatggacgac ttcgaggccc aggccgcccg
     4561 tttcggggcc gagatgcgcc cgga
//
LOCUS       DBNGVD010000459         5076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4118567, whole genome shotgun sequence.
ACCESSION   DBNGVD010000459 DBNGVD010000000
VERSION     DBNGVD010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4118567"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="AC16CM_12365"
     CDS             complement(<1..1573)
                     /locus_tag="AC16CM_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3140672.1"
                     /translation="MTRSLVAWRGARGKTGGGGERRGNLPRGSGHRIPPLAGVATLAC
                     VVLAALTMAEGLVSAGVIRHGVSVGDVALGGKTPAEARVALQARYPDGSGRLSLSGPT
                     GTIGIPEEKLGARLDVRATVEKAYAVGRRGSLPERLIGRVESPFGTSVPAELHLRPGA
                     ARAAVEEISGRMNREARDAAVDVNGSTVLVSGAETGYRLDVPATAREIEQALAELSPE
                     ARTSGRVIQPRLTTEEAEAAAREARRAMSGPLVLTAPDESWKLSPAEVGSALIVARKG
                     GTLEIGLDLDRLRRQLSGMYADLTVKPVEAGYEVKGGADPTVSVTPGREGRKIEEDEL
                     FHSILKGIFEGKRRYSVTTVAEKPDLTTARAVRLAPTDLLGSYRTNYAVVRDASTRVE
                     NLAISSGAVNGTFLAPGEVFSMNSEVSHLDYEQTKVIVGGQETTADGGGLCQVTSTLY
                     NAANFAGLDVIERTPHSSQLPYIRPGMDATVWWGGPGKADDLDMKFRNTTDGYVLLRE
                     RVARDGYVYAEIWGRP"
     gene            complement(1807..2139)
                     /locus_tag="AC16CM_12370"
     CDS             complement(1807..2139)
                     /locus_tag="AC16CM_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140673.1"
                     /translation="MRLRETSLKLLPVLVVAWATLIMLGGALYLARHAGPPDTGQAVK
                     AGLGLCAISVAVLAGKVVRRVAPPAHTYGLLASSSARPPDGEVSCFLSGPPATGPPIF
                     RLLRVSRT"
     gene            2273..2623
                     /locus_tag="AC16CM_12375"
     CDS             2273..2623
                     /locus_tag="AC16CM_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3140674.1"
                     /translation="MTGYTKRRLSSAAMGMVAERFKVLSEPMRLRLLYSLMDGEKNVS
                     ELVAETGSAQANVSRHLSVLLDAGILGRRKEGTSTHYHIADESIFELCNLVCGSIYDR
                     LAAELDELDAPPRH"
     gene            2719..2928
                     /locus_tag="AC16CM_12380"
     CDS             2719..2928
                     /locus_tag="AC16CM_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140675.1"
                     /translation="MDDGIKFLVGALSGALVVLLLLTGTISGGMMGGMGQMMGGTLGM
                     AFGLVFWFALVALIVLLVACAVRRI"
     gene            2989..3408
                     /locus_tag="AC16CM_12385"
     CDS             2989..3408
                     /locus_tag="AC16CM_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR3140676.1"
                     /translation="MEDFTPVSGLAGGMLIGLATVLLMLFNGRIAGISGIVGGLMARK
                     GYGVGWRAMFAGGLLLGAVGYMLATGGNFPVQIQVSSPIVVVAGLLVGFGSRLGSGCT
                     SGHGVSGIARLSGRSIVATLVFMGTAIVTVFLTGHVL"
     gene            3429..3842
                     /locus_tag="AC16CM_12390"
     CDS             3429..3842
                     /locus_tag="AC16CM_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR3140677.1"
                     /translation="MAVALFSGTLFGLGLAVSGMANPQKVLGFLDVAGGWDPTLAFVM
                     GGALLVAFPALHLVFGRGRPVLAEDFELPATTRVDARLVGGSVLFGVGWGLSGFCPGP
                     AVVALVPALTTGLLPAFAFAAAMVAGMVLYEWISR"
     gene            3897..>5076
                     /locus_tag="AC16CM_12395"
     CDS             3897..>5076
                     /locus_tag="AC16CM_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3140678.1"
                     /translation="MFFREVLNEDLGCASYIIADGGEAVVVDPRWEVGEYLRIAEDNG
                     FRISHILETHNHADHLSGKGRLVEATGARIHISGEAGVEYEHEPLEDGDQIEVGRVLI
                     TALATPGHRPEHLAFTVEDASRGGQPWVLFTGDSLFVGDLARPDLAVEAEEGARGLFR
                     SLGKLENLADFVEVRPGHIGGSLCGGAHMSRKPGSTIGFERRYNDYLRFEDEDEFVRT
                     LTSEQTPQPPNFGRIVELNRGPLLTGSIMPVPLLPQRVAELVGDGAVLIDGRDQREFD
                     AVHVPGSINVTTNQTGVGTRAAWMVEPESEVIVTAGGEDEARAMIPMLEAVGFRRVRG
                     FLTGGLNAWRAAGLETQATPALDIPGLAEKIENDQVTVLDVRNAAEWETGHVDGSIHV
                     P"
BASE COUNT          863 a         1630 c         1661 g          922 t
ORIGIN      
        1 tgggccggcc ccagatctcg gcgtacacgt agccatccct ggccacccgc tcgcgcagca
       61 gcacgtaccc atcggtggtg ttgcggaact tcatatcgag gtcgtcggcc ttgcctggac
      121 cgccccacca caccgtcgcg tccatccccg gacgtatgta ggggagttgt gaggagtgcg
      181 gggtgcgttc gatcacgtcg aggccagcga agttggcggc gttgtacaac gtggaagtca
      241 cctggcagag ccctcccccg tccgcggtgg tctcctgccc gcccactatg accttggtct
      301 gctcatagtc caggtgggag acttcggagt tcatggagaa gacctcgcca ggggccagga
      361 aagttccgtt cacagcgccc gaggagatgg ccaggttctc aacccgcgtg ctggcgtcgc
      421 gcacgacagc atagttggtg cggtaagaac ccaggagatc ggttggcgcg agtcgtacgg
      481 ctcgagcggt ggtaaggtcc ggtttttcgg ccaccgtggt aacggagtat ctgcgcttgc
      541 cctcgaagat gcccttcagt atggagtgaa acagttcgtc ttcctctatc ttcctgcctt
      601 cccgccctgg cgtaacggag acggtcgggt ccgccccacc tttcacctcg tagccggcct
      661 cgacgggctt taccgtcagg tccgcgtaca taccggacag ttgccggcgc aaacgatcga
      721 gatccaaccc aatctctagc gtcccaccct tccgggccac aatgagcgct gatcccacct
      781 cggcaggcga gagtttccag ctctcgtccg gcgccgtcag cacgagcggc cccgacatcg
      841 cccggcgtgc ctctcgcgcg gcggcctcgg cctcttcggt ggtaagacgc ggctggatca
      901 cccgcccgga tgtccgcgct tcgggagaga gttcggcaag cgcttgctct atctctcggg
      961 cggtagccgg aacgtcgagt ctgtagcccg tctctgctcc ggagacaagg acggtagacc
     1021 cgttcacgtc gacggctgcg tcgcgtgctt ccctgttcat gcggccggag atttcttcga
     1081 cggccgcgcg cgctgctccc ggacggaggt gtaactcggc gggtacgctc gtgccgaagg
     1141 gtgattcgac ccgtccgatc aggcgctccg gaaggcttcc ccgacggccg accgcgtagg
     1201 ccttctcgac ggtcgcacgg acatcgaggc gagcacccaa cttttcctct ggaatgccaa
     1261 tggtccctgt aggaccggag agggagaggc gccccgatcc atcagggtac cgtgcttgga
     1321 gcgccacacg ggcctccgcc ggggtcttgc cccccaacgc gacgtcgcca acggagacac
     1381 catggcggat cacacccgcg ctgaccaacc cttccgccat ggtgagcgcg gccagcacga
     1441 cgcacgcgag ggtcgccacc ccggcgagtg gggggattct atggcccgat ccccggggga
     1501 gatttccgcg acgttctccc ccgccccccg ttttgccgcg cgccccgcgc caggccacca
     1561 ggctcctagt catgactggc acctcctcgc accatcgtct cgacggtatt cggcccagcg
     1621 gcgaccgcct tctcttcgta cttcctggca ggcccgttca tacgagccac ctccttcggg
     1681 taggctaaac gtaaggattg cgtcctcgtc acgcccaggc acgatcgcca tgcctgaacg
     1741 cgtcacaacg tacgccctgc agtcgatggg agcgcaagcc ccgatcgacg atgcgggcga
     1801 gaatccctac gttcgcgaga cccgaaggag cctgaagatg ggtggcccag tcgccggcgg
     1861 accagagagg aagcaagaga cctccccatc cggcggacgg gccgatgatg acgcgaggag
     1921 gccgtacgtg tgcgcaggcg gcgccacccg gcgcaccacc ttgccggcca gaacggccac
     1981 gcttatcgca cacaatccaa gcccggcttt gacggcctgg ccggtgtctg gcgggccggc
     2041 gtgcctggcc aggtagaggg cgccgccaag catgatgagg gtcgcccagg cgaccaccag
     2101 cacgggaagc agcttcaaag acgtttcgcg cagcctcatc acatcagcct acatggtaca
     2161 cgatggatcc cggtattcgt gcgcagcggc ccacagcaca cgtagtagaa tcgctatatc
     2221 gctctatagc gatataatgt accggcaagc caaatgcgaa acggaggtcg ggatgaccgg
     2281 atacacgaag cgaaggttgt cgtcagccgc catgggcatg gttgcggaac ggttcaaggt
     2341 actctcggag ccgatgaggc tcaggcttct ctactccctc atggacggcg agaagaacgt
     2401 ctccgagctc gtagcggaga ccggaagcgc ccaggccaac gtctccagac acctctcggt
     2461 gctgctcgac gccggcatcc tgggccgcag aaaagaaggt accagcaccc actaccatat
     2521 tgcggacgag tcgatcttcg agctctgtaa tctcgtgtgc ggctccatct acgaccggct
     2581 ggcggccgaa ctcgacgagc tcgacgctcc cccccgacac taaacccccg agagcaagac
     2641 acatgccctg cgtgccttga cactccttta tatcgttata taacgatata gtgttagaag
     2701 agagcaggag tacagactat ggacgacggc atcaaattcc tcgtgggggc gctctccggc
     2761 gcccttgtcg tgttgctgct tctgacgggg accatctccg gcggaatgat gggaggcatg
     2821 gggcagatga tggggggcac gctcggcatg gcgttcgggc tcgtgttctg gtttgcgtta
     2881 gtcgccctga tcgtgctcct cgtcgcctgc gccgtccggc gcatatagcg tggctacggt
     2941 ccgcggttcg ggcgtagtgg aggaagagat gtacagaggg gggatatctt ggaggacttt
     3001 acaccggtaa gcggtctcgc cggtggaatg ctcatcggcc ttgccaccgt gctgctcatg
     3061 ctgttcaacg gccgcatagc cgggatcagc ggcatcgtcg gcggcctgat ggccaggaag
     3121 ggttacgggg tcggctggcg cgcgatgttc gcgggcggcc tcttgcttgg cgccgtgggt
     3181 tacatgctgg caacgggagg gaatttcccg gtccagatcc aggtctcttc accgatcgtg
     3241 gtcgtggccg ggctcctcgt gggcttcggg tcccgcctcg gttccgggtg caccagcggc
     3301 cacggcgtca gcggcatcgc gcgcctgtcc gggcgctcga tcgtggcgac gctcgtgttc
     3361 atgggaacgg cgatcgtgac ggtgttcctc acaggtcatg tgctgtgagg aggttcgggt
     3421 tgccagggat ggccgtagcc ctgttctcgg gcacgctctt cgggctcggc ctcgccgtct
     3481 cgggtatggc gaacccccag aaggtccttg gttttctcga tgttgcggga ggatgggacc
     3541 cgacgctcgc gttcgtcatg ggcggggcgc tgctcgttgc ctttccggcc cttcacctgg
     3601 tcttcgggcg tgggcgtccg gtactggcgg aagacttcga gttgccggcc actaccaggg
     3661 tcgacgcccg cctcgttggc ggttcggtgc tcttcggcgt cggctggggg ctctctgggt
     3721 tctgtccggg tcccgcggtc gtggcgctcg ttccggcgct gaccacgggt ttgctgccgg
     3781 ccttcgcctt cgccgccgcg atggtggctg gcatggtcct ctacgagtgg atctcgagat
     3841 gatcgggcgc gagaagcgaa caagccgata accaacgagc ctggaggtcg tagaacatgt
     3901 ttttcagaga agtcttgaac gaggacctgg gatgcgcttc gtacatcatc gccgacggcg
     3961 gggaggcggt tgtagtggat cccagatggg aggtgggaga gtacctcagg atagccgagg
     4021 ataacgggtt tcggatctcc cacatcctcg agacccacaa ccacgccgac cacctctcgg
     4081 gcaagggacg cctcgtcgag gccacgggtg ccaggataca catctccggg gaggcgggcg
     4141 tcgagtacga gcacgaaccc ctcgaagacg gagaccagat cgaggtcggc agggtgctga
     4201 tcacggccct tgccacgcca ggccaccgtc cggagcacct cgctttcacg gtagaggacg
     4261 cgagccgggg cgggcagccc tgggtgctgt tcaccggcga ctcgctcttc gtgggggatc
     4321 ttgcccgacc ggacctcgcc gtcgaggccg aagagggtgc ccgcggcctg ttccgttcgt
     4381 tgggaaaact cgagaacctc gcggacttcg tggaggtgag gcccgggcat ataggcgggt
     4441 cgctgtgcgg tggcgcgcac atgagccgca agcccggttc caccatcggc ttcgagcgcc
     4501 gctacaacga ttacctgcgc ttcgaggacg aggacgagtt cgttaggacc ctgacctccg
     4561 aacagacgcc ccagccaccg aacttcgggc gcatcgtgga gctcaaccgg ggacccctcc
     4621 ttaccgggtc catcatgccc gttcccctct tgccgcagcg cgtcgccgag ctcgtcgggg
     4681 acggggcggt cctgatagac ggccgtgacc agagggagtt cgacgcggtc cacgtcccgg
     4741 gctcgatcaa cgtcaccacc aaccagaccg gggttggcac gcgggcggcc tggatggtgg
     4801 aacccgagag cgaggtcatc gtaacggccg gtggcgaaga cgaggcaagg gccatgatac
     4861 ccatgctcga agccgtgggg ttccgccgcg tacgcggctt tctcaccggc ggcctgaacg
     4921 cctggcgcgc ggccggcctg gaaacacagg ctacccccgc cctggacata ccgggcctcg
     4981 ccgagaagat cgaaaatgac caggtcaccg ttctcgacgt caggaacgcg gcagagtggg
     5041 agacgggaca cgtggatggc tctattcacg tcccct
//
LOCUS       DBNGVD010000460         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_292437, whole genome shotgun sequence.
ACCESSION   DBNGVD010000460 DBNGVD010000000
VERSION     DBNGVD010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_292437"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1062)
                     /locus_tag="AC16CM_12400"
     CDS             complement(97..1062)
                     /locus_tag="AC16CM_12400"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016865514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3140679.1"
                     /translation="MPGALVEGEWKTEQQWQNTDGRFVRQATSFRDRVSADGSSGFPA
                     EAGRYHLYIAWACPWAHRTAIMRRLKGLEDVIGLSAVGSYMGEDGWAFHDEPGVIPDT
                     VNGAHFLREVYVKADPDYTGRVTTPVLWDKETGTIVNNESRELVRMLDTEFEGLAKTG
                     ADFLPPDLLGEVDETIDAIYEPVNNGVYRSGFATNQEAYEEAVTDVFEALDHWEDVLS
                     RRRYLCGDRITEADWCMFPTLVRFDAVYHGHFKCNLRRIVDYPNLWGYVRGLYQQPGV
                     AETVNMDHIKRHYYRSHDNINPTRIVPKGPVLDFTEPHDRAVLYA"
     gene            complement(1251..2150)
                     /gene="rapZ"
                     /locus_tag="AC16CM_12405"
     CDS             complement(1251..2150)
                     /gene="rapZ"
                     /locus_tag="AC16CM_12405"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3140680.1"
                     /translation="MVERGTVPGEVGRRKVVVITGLSGAGKSNALQAFEDAGYFCVDN
                     LPPRMIPAVMETTGQGDEGPEGLVVVVDIRGRRYFGEELERSLEAVERAEGWEHRILF
                     IDSDDASLLRRYKESRRPHPAARNGDVLAAIKSERHDLANVREMADLVIDTSNYSALD
                     TRLRFKRLAESLSGRMTVSLISFGFKHGTPLDVDTLLDVRFLPNPHYDPQLRPLTGHD
                     APVREAVLGAEDCRQFLHKTYDLLSFLIPRYAAEGKTYFTLGIGCTGGRHRSVAITEE
                     LARRLKEQDAEIDLFVRHRDVEK"
     gene            complement(2502..4340)
                     /gene="uvrC"
                     /locus_tag="AC16CM_12410"
     CDS             complement(2502..4340)
                     /gene="uvrC"
                     /locus_tag="AC16CM_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3140681.1"
                     /translation="MSENGYPDRSHLPTSPGVYIFKDAKDRVIYVGKAKNVRSRVANY
                     FTRSGDGRPKIAELRERVSQIDFIVTESETEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVVTTGDKFPRVFATRAAHDPRHRYFGPFPSAGSVHSTLDVLNKTLPFRKCRGPEPG
                     RRSGVPCLNYHIGRSVAPCIEAVTKGEYDEIISDVIDFLEGRVGGLIRDREEAMRVAA
                     REMDFERAARLRDEVAALTHVRDRQQATIASEDSFDAFGAFTENESACVQVFAVREGQ
                     VVNRDSFLLDNYGESSAEDVALQFVPQYYGTAAVPREVLVQSDERGDELATLAEHLTS
                     LRGTRVEVRRPRRGDKRRILEMSLRNAEMGLEHERALDEARRNRVASTLDGLLEELAL
                     PKLPVRIECYDISNTMGTNSVASMVVFQGGRPAKSQYRRFRIRTVEGADDFSSMAEVI
                     RRRLQRLKENDDKFTPAPDLILLDGGKGQLSAVAPILEDMKVGDELPDIALRSLAKRD
                     EEVFEPGRPEPVILQRNSPELHLLQRVRDEAHRFANTYHRELRGRAMTASVLDDLPGV
                     GPARKKQILTHFGTPEAFLNASLEELEGVPGLPGRVAREVYAKVHR"
     gene            complement(4414..>5070)
                     /locus_tag="AC16CM_12415"
     CDS             complement(4414..>5070)
                     /locus_tag="AC16CM_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3140682.1"
                     /translation="SGAGVRISASSSNKVQGNSIGKTRDGSLLGNGRSGVEISGADDN
                     LVGGTVSGASNDIAGNGVGLSGGNGVTVSGAGSTGNRILRNSIADNDDLGVDLGIAEF
                     GDGITLNDPQDADTGANNLQNFPVLTAVAHSRGKTTIKGKLNSGPDRTFTIQFFSNPS
                     GDEGKKFVGQKSVTTNANGNVSFEFGPAQAVPMGQTVTATATNPGGNTSEFSAPRTAA
                     "
BASE COUNT          821 a         1604 c         1605 g         1040 t
ORIGIN      
        1 agcaacacgg gcaggccggt gccaccgccg aacgctacta ccttcaaatc ctcgacgcta
       61 cgcatgggcc ccgagtatac cgccggccga aaacgcctag gcgtagagca cggcccggtc
      121 gtgcggctcg gtgaaatcca gaaccggccc cttcggcact atgcgggtgg ggttgatgtt
      181 atcgtggctc ctgtaatagt gcctctttat gtgatccatg tttacggtct cggccacgcc
      241 tggctgctgg tagaggcccc tcacgtaccc ccacaggttc ggatagtcca cgatccgtct
      301 caggttgcac ttgaagtgtc cgtggtatac ggcgtcgaaa cgcaccagcg ttgggaacat
      361 gcaccagtcg gcctcggtta tacgatcgcc gcacaggtag cgccttctgg agagaacgtc
      421 ctcccagtga tccagcgctt cgaagacgtc ggttacggcc tcttcgtagg cctcctggtt
      481 cgtggcgaag ccggagcggt agacgccgtt gtttacgggc tcgtagatgg cgtcgatggt
      541 ctcgtcgacc tccccgagca ggtcaggggg caagaaatcc gcgcccgtct tggctagccc
      601 ttcgaactcc gtgtcgagca tgcgcacgag ctcgcgcgac tcgttgttca cgatcgttcc
      661 cgtctccttg tcccacaaca ccggggtggt aacgcgcccg gtgtagtcag ggtcggcctt
      721 gacgtagacc tcgcgcagaa agtgtgcgcc gttcaccgtg tcgggtatga cgccgggctc
      781 atcgtggaaa gcccagccgt cctcacccat atacgagccg acggcggaga gaccgataac
      841 atcctccagg cccttcaatc ggcgcatgat cgccgtccgg tgcgcccacg ggcacgccca
      901 tgcgatgtac agatgatacc ggccagcctc ggcgggaaac ccactgctcc cgtcggccga
      961 gacccgatcc cggaaactcg tggcctggcg cacgaaccgc ccgtccgtgt tctgccactg
     1021 ctgctctgtc ttccactcgc cttcgaccaa cgctccaggc attgggcctc ccttcgttcg
     1081 gcggctaatg gttattagct ctcagccgtc agctttttgc agatggtgtt tcttggcgtg
     1141 ataactgagt ctatccgatg gcgttgcggc aggagagcgc gaagtcgtag gttgctgact
     1201 gcagattgct gacgactaat tgctgaaagc tgaaagctga ttgctcgaag ctacttctcc
     1261 acgtccctgt gtctcacaaa gaggtctatc tccgcgtctt gctccttgag acgtctggcg
     1321 agttcttccg ttattgccac ggagcggtgt cttccgccgg tgcagccgat acccagggtg
     1381 aagtaggtct tgccttctgc tgcgtagcgg ggtatgagga aggataggag gtcgtaggtc
     1441 ttgtgcagaa attgcctgca gtcttcggcg ccgagtacgg cctcgcgcac gggcgcatcg
     1501 tggccggtca ggggtctgag ctggggatcg tagtgtgggt ttggtaggaa gcggacgtcg
     1561 agcagggtgt ccacgtccag cggggtaccg tgcttgaagc cgaaggagat caggctgacg
     1621 gtcatcctgc ctgagagcga ctcggcgagc cgcttgaagc gcaggcgggt gtcgagcgcg
     1681 gagtagttgg aggtgtcgat tacgaggtcg gccatctccc tgacgttggc gaggtcgtgc
     1741 ctctcgctct tgatggccgc cagcacgtca ccgttgcggg cggccggatg cggcctgcgg
     1801 ctctccttgt agcgcctcag aagcgaggca tcgtcggagt cgatgaacag gatgcggtgc
     1861 tcccagccct ctgcccgctc cacggcctcc aggctacgct ccagctcctc cccgaagtac
     1921 cgcctccccc tgatatccac gaccacgacc agcccctcgg gaccttcgtc cccctggccc
     1981 gtggtctcca tcaccgccgg gatcatgcgc ggcggcaagt tgtccacgca gaagtacccc
     2041 gcatcctcga aggcctgcaa ggcattgctc ttgcccgccc cggaaagccc ggtaataacg
     2101 acgaccttgc gcctgcccac ctccccgggg acggtacccc gctcaaccac gccgcatcat
     2161 ccgggcttca tggggcaaac gaaccatctc ggaggtcgag attagcacca tggatcatgg
     2221 cttgcaagca cgcttcggct tcgctccgct tgtcagcaca ctacggccta tcggcctccg
     2281 tttgtcagca cgcttcgacc ctgcgggtct cgcttgtcgg caacgcatcg gcttcgcctc
     2341 gctttcggct ggtcgtctcg gttcgcatcg gtggctccga taccccacct ttcccaggca
     2401 tccaaccttc agagcggaag ggtgcagtcc gcagagggat ctggctgaca gcgagctcta
     2461 ggcgaagcgc cgtgctgaca aggctcgaag ggcgtagcgt gctacctatg caccttcgcg
     2521 tacacctcgc gagcgaccct tccaggtagt cccggaaccc cctccagctc ttccagcgag
     2581 gcgttcagga aagcctcggg ggtgccgaaa tgggtcagga tctgtttctt gcgggctggg
     2641 ccaacgccgg ggaggtcgtc cagtacggag gcggtcatgg cgcggccgcg cagctcgcgg
     2701 tggtaggtgt tggcgaagcg gtgggcttcg tcgcggacgc gctgaaggag atgcaattcg
     2761 ggagagttgc gctgcaggat gaccggctcg ggacggcccg gctcgaagac ctcttcgtcg
     2821 cgcttggcga gcgagcgaag cgctatgtcg gggagctcgt cacccacctt catatcttcc
     2881 aggatggggg ccacggcaga gagctggccc ttgccaccgt ccagcaggat caggtcggga
     2941 gccggggtga acttgtcatc gttctccttc aggcgctgca ggcggcgacg gatcacctcg
     3001 gccatgctcg agaagtcgtc ggcgccctcg accgtgcgta tcctgaacct gcggtactgg
     3061 ctcttggccg gccttccgcc ctggaacacg accatcgagg ccacggagtt ggtccccatg
     3121 gtgttggaga tgtcgtagca ttctattcgg acgggaagct tggggagggc taactcctcc
     3181 agcaaaccgt ccagcgtgga ggcgaccctg tttcgcctgg cctcgtcaag cgcccgctcg
     3241 tgctcgaggc ccatctccgc gtttcgtagg ctcatctcca ggatgcgccg cttgtcgccc
     3301 cgcctgggcc gtcgcacctc tacacgtgtg cccctcaggc tcgtgaggtg ctcggccagc
     3361 gtcgccagct cgtccccgcg ctcgtcggac tggactagca cctcccgcgg gacggccgcc
     3421 gtgccgtagt actggggcac gaactggagt gccacgtcct cggccgatga ttcgccgtag
     3481 ttatccagga ggaacgagtc gcggttcacg acctggccct cgcgcacggc gaagacctgc
     3541 acgcacgctg actcgttctc ggtgaaggcc ccgaaggcgt cgaaggaatc ctcagaggcg
     3601 atggtggcct gctgccggtc gcgaacgtgg gtcagggcgg cgacctcgtc gcgcagcctg
     3661 gcggcccgct cgaagtccat ctcgcgggcg gccacgcgca tggcctcctc acggtcccgg
     3721 atcagaccgc ccacccgtcc ctcgagaaag tcgatcacgt cagagataat ctcatcgtac
     3781 tcccctttgg tgacggcctc gatgcagggt gccaccgagc gcccgatgtg gtagttgagg
     3841 cagggcaccc cgctcctgcg tcccggctcg ggtccccggc atttgcggaa agggagggtc
     3901 ttgttcaaga cgtcgagcgt cgagtgtacg ctgccggcgc ttggaaaggg cccgaagtag
     3961 cggtggcgtg ggtcgtgggc ggcccgtgtg gcgaaaaccc tcgggaactt atcgccggtc
     4021 gtgaccacga tataggggta gctcttgtcg tccttgagcg aggcgttgaa gcggggcctg
     4081 tggcgcttga tcaaattagc ctcgagtacc agcgcctccg tctcgctctc cgtgacgatg
     4141 aagtcgatct ggctgacgcg ttccctgagc tccgctatct tgggacgccc gtcgccagag
     4201 cgcgtgaaat aattcgccac cctgctgcgt acgtttttgg ccttgcctac gtagataacc
     4261 ctgtccttgg cgtccttaaa gatgtagacg cctggcgagg tcgggaggtg ggatctgtca
     4321 ggataaccgt tctcggacat cgttggagat tataccgcga cgagcccaac ctctcgtcag
     4381 gcttctgtgg ggatggcccg ctcgatgctg atactatgcc gctgttcttg gcgccgagaa
     4441 ttccgaggtg ttaccacccg ggttggtggc cgtcgcggtt acggtctggc ccatcgggac
     4501 cgcctgggcc ggtccaaatt cgaaggagac attgccgttc gcgttagtgg tgacgctttt
     4561 ctgtcccacg aacttcttgc cttcgtcccc tgatggattg gagaagaact ggatggtgaa
     4621 ggtcctgtcg ggcccgctgt tgagtttgcc tttgatcgtg gtcttgcccc gcgagtgggc
     4681 gaccgcggtc aggaccggga aattctggag gttgttggca ccagtgtcgg catcttgcgg
     4741 gtcgttgagg gtgataccgt cgccgaactc ggcgataccc aggtcgacgc ccaggtcgtc
     4801 gttgtcggct atggagtttc gcaggatacg gttgcccgtg ctgcctgcgc cgctgaccgt
     4861 taccccgttg ccgccagaga gcccgacgcc gttgcccgcg atgtcgttgg aagcccccga
     4921 gaccgtgccc cccaccaggt tgtcgtcggc cccggagatc tctactccac ttcggccgtt
     4981 gcccaacagg cttccgtccc tggtctttcc gatggaattg ccttgcacct tgttggagga
     5041 ggaagcggag atgcggaccc cggcgccgct
//
LOCUS       DBNGVD010000461         5595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1386543, whole genome shotgun sequence.
ACCESSION   DBNGVD010000461 DBNGVD010000000
VERSION     DBNGVD010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1386543"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..306)
                     /locus_tag="AC16CM_12420"
     CDS             complement(58..306)
                     /locus_tag="AC16CM_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FitA-like ribbon-helix-helix domain-containing
                     protein"
                     /protein_id="HIR3140683.1"
                     /translation="MSKMIQIRNVPDDLHRTLKARAARAGMTLSDYLLSEIELVAEKP
                     TMSEWLEKVRSREPVKLDEPPDVTIRRFRDADDPRNSS"
     gene            406..903
                     /locus_tag="AC16CM_12425"
     CDS             406..903
                     /locus_tag="AC16CM_12425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3140684.1"
                     /translation="MDLRSVQRPLKKKYRDEPGSSRITLEAKASQEDTPISCSVDVGK
                     AVYEAGAHEGVGGTGEVACSGDLLLGALAACAQVTCQMVAGSMGIVAEKIEVTVEGDM
                     DLAGTLGVSREAPVGFESIRTKIEIVAPGATEDQMEKLRKSTEQYCVVYQTLAKTPEL
                     KTEWT"
     gene            951..1916
                     /gene="meaB"
                     /locus_tag="AC16CM_12430"
     CDS             951..1916
                     /gene="meaB"
                     /locus_tag="AC16CM_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3140685.1"
                     /translation="MKNGDLVERLLAGDRRALARVISRIEREDEETARIIGEIYPETG
                     DALTVGFTGPPGVGKSSIIAKLIELYREEGKRVGVISVDPSSPFSRGAILGDRIRLSD
                     HFLDPGVFIRSMGSRGHLGGLAGGSRLAAIAMEACGYDVILYETVGVGQGEVEVASAA
                     DTVVLCLQPGAGDAVQALKAGVMEIADVFCINKSDHPRARNTASEVRSILEIGHELDP
                     DPWFPPIVMTRGNTGEGIRELKEKIQDHRDFLEEDGRLADRRRAALRDFVVAWATNRL
                     EKEMHERLNREDTGLVEKVYNKELDPISASEQIFKRVQARSQSGL"
     gene            complement(1913..2377)
                     /locus_tag="AC16CM_12435"
     CDS             complement(1913..2377)
                     /locus_tag="AC16CM_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR3140686.1"
                     /translation="MAALKIFAGLGCGIIFGLGLAISGMMNPARVIGFLDVAGDWDPT
                     LAFVMGGALLVAAPAYRLILGRGRPILDDSFSLPKNTLLDKPLILGSALFGVGWGLVG
                     FCPGPAVAAVVTGLPAVLGFLAAMLAGMALHAWISSRQPVRKGSPEAAPRTR"
     gene            complement(2377..2796)
                     /locus_tag="AC16CM_12440"
     CDS             complement(2377..2796)
                     /locus_tag="AC16CM_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR3140687.1"
                     /translation="MADFTPASGLLGGLLIGLAVALTLLLNGRIAGISGIVGALLNPK
                     AGDRGWRAAFVAGLPLGALAYILVEGGPAPVDVLASPPAILVGGLLVGFGTRMGAGCT
                     SGHGVCGLALLSWRSAVATAVFFVVAMLTVFLTRHVF"
     gene            complement(2865..3371)
                     /locus_tag="AC16CM_12445"
     CDS             complement(2865..3371)
                     /locus_tag="AC16CM_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HIR3140688.1"
                     /translation="MKGPAGLRSRAAGPGDAEAIARIYNEGIEERIATFETRPRAVKE
                     IMGWFDDGHPIVVVEGEGGVISFANASAYSPRECYSGVAEFSVYTAGEARGLGAGTLA
                     MEALISTAEQAGFWKLTSRVFVENGPSLNLLGSLGFREVGVHEKHARLDGEWRDVVVV
                     ERLIPANL"
     gene            complement(3402..3761)
                     /locus_tag="AC16CM_12450"
     CDS             complement(3402..3761)
                     /locus_tag="AC16CM_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR3140689.1"
                     /translation="MPDLRPFAGEEYCYLTTTGRVSGRPHEIEIWFSLVPETGTLYML
                     SGGGDRSDWVKNLRRDPGVMIRIAGEDLGGVARDVRDADEDELARRLLVEKYERSPGR
                     LSNWRRTSLPVAVDLSA"
     gene            complement(3864..4097)
                     /locus_tag="AC16CM_12455"
     CDS             complement(3864..4097)
                     /locus_tag="AC16CM_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140690.1"
                     /translation="MSNIGDYEETYANFGWDTPERFNFGRDVVDRWAAQDRVMVLLGK
                     EQTAPYRYPRQIGSIEELPKTTSGKIRRVELRG"
     gene            complement(4196..>5595)
                     /locus_tag="AC16CM_12460"
     CDS             complement(4196..>5595)
                     /locus_tag="AC16CM_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3140691.1"
                     /translation="SVGGPGNIIANNHIGTDADAAEDLGNTLSGVFVDNTADTLVGGS
                     TLDGSLNIISGNDRHGVEVSNIGAEGTRIVGNLIGLNRNGGSSNVGNSRDGVRVSSTP
                     RVEIGDTLAGAGNAISANGEHGVEIVGTSSTNNVVLGNVIGTNSNGASDFGNLGDGIR
                     IGNSSGVTIGGTVAGARNVISGNDANGIFISGTSSATGNRVQGNFIGTDRNGTTGFGN
                     SEAGVRVETPGNLIGGTATGAGNVISANGDEGVHIFGASAENNVVQGNKIGTDAGGVS
                     NLGNLHGVFITGASGNTIGGTVDAAANKIALNRSRGMVVVGDAATGNSFLRNSIFGNG
                     TPPGLSIDLGNDGVTANDTSDPDTGPNGLQNFPVLTSASETRIEGRLNSRPGRTFTIQ
                     FFSNPPPISPLLVREGTTFLGERNVVTNSEGRVSFAFNTQVNTGELVTATATIITNNS
                     GPGNTSEFSATRLVE"
BASE COUNT         1013 a         1827 c         1784 g          971 t
ORIGIN      
        1 gccgaggttg agcaacacgg cgatcgccgc agaggcatcc aggacgatca aggtcagcta
       61 gctcgagttg cgcggatcgt cagcgtccct gaaccgccgg atagtcacgt ccggcggctc
      121 gtccaacttg accggctccc tgctccttac cttctccaac cactcgctca tggtcggttt
      181 ctctgccaca agctctatct cggaaagcag gtagtcagat agcgtcatgc ccgccctggc
      241 cgctcttgcc ttcagggtgc ggtgcagatc gtcgggtacg tttcgtatct ggatcatctt
      301 cgacatgtaa ttcagagtaa cacatgcgct gcacatgcac gaaatgtact ttcttctggc
      361 ggctacaata ggctctggaa gggaagctag ctctggagga cgatcttgga tctccgctcg
      421 gttcaaaggc cgctaaagaa aaagtaccgt gacgagccgg gatcctcccg cataaccctg
      481 gaggcgaagg ctagccagga ggacaccccg atctcctgct ccgtcgacgt cgggaaggcc
      541 gtctacgagg ccggggctca cgagggcgtg ggggggacgg gcgaggtcgc ctgttccggc
      601 gacctcttgc tcggcgctct ggcggcctgc gcccaggtca cctgccagat ggtcgccggg
      661 tccatgggaa tagtcgccga gaagatcgag gtgaccgtcg agggggatat ggacctcgct
      721 ggtacgctgg gcgtctcccg ggaggccccc gtcggcttcg agtccatccg caccaaaatc
      781 gaaatcgtgg cgcccggggc gaccgaagac cagatggaaa agctgcgtaa gagcacggag
      841 cagtactgcg tcgtctacca gacgctggca aagaccccag agctcaagac cgagtggacg
      901 taggccgacc cggcaataag ccagcgcgcg tagtctcgat gaggtcgtag atgaaaaacg
      961 gcgacctcgt ggaacgtctg ctcgctggtg accggcgggc gcttgcgcgg gtgatctcca
     1021 ggatagagcg cgaggacgag gagacggcga ggataatcgg ggagatatac cccgagaccg
     1081 gcgacgccct cacggtcggc ttcacgggcc cgcccggcgt cggcaagagc agcatcatcg
     1141 cgaagctcat cgagttgtac agggaggagg gcaagcgggt aggggtcatc tccgtcgacc
     1201 cttctagccc gttctccaga ggggccatcc tgggtgaccg catcaggctc tcggaccact
     1261 ttctcgaccc cggtgtgttc atacggtcga tggggagcag gggtcatctg ggcggactcg
     1321 ccgggggctc gaggctcgcg gccatcgcca tggaggcctg cgggtacgac gtgatcctct
     1381 acgagaccgt tggcgtggga cagggtgagg tcgaggtcgc ctcggccgcc gacacggtgg
     1441 ttctgtgttt gcagccggga gccggggacg cggtgcaggc gctcaaggct ggggtaatgg
     1501 agatagcaga cgtcttctgc atcaacaaat cggaccaccc gcgggcaagg aacaccgcct
     1561 cggaggtgcg ctccatactg gagatcgggc acgagctcga ccccgacccg tggttcccgc
     1621 ccatcgtgat gacccgcggc aacaccggcg aaggcatacg ggaactgaag gagaaaatcc
     1681 aggatcaccg cgactttctc gaagaggacg gcaggctggc cgacagacgg cgggccgccc
     1741 tcagggactt cgtagtcgcg tgggccacga accgccttga gaaggagatg cacgagcgcc
     1801 tgaaccgcga ggacaccggt ctcgtggaga aggtctacaa caaggagcta gaccccatct
     1861 cggcctcgga gcagatcttc aagcgcgtcc aggcccggtc ccagagcggt ctctagcggg
     1921 tacgcggggc tgcctcaggc gatcccttgc gcacaggttg cctgctggag atccaggcgt
     1981 gcaatgccat gccggcgagc atggccgcca ggaacccgag gacggcgggg agcccggtaa
     2041 cgacggctgc caccgcggga cccggacaga acccgacgag cccccacccg acgccgaaga
     2101 gcgccgagcc gaggatcaag ggcttgtcta gcaacgtgtt cttgggaagc gagaacgaat
     2161 catccaggat gggacgcccg cggcccagga tcagacggta ggcgggcgcg gcaacgagca
     2221 gggcgccgcc catgacgaag gcgagggtgg gatcccagtc tccggccacg tcgaggaagc
     2281 cgatgaccct ggccgggttc atcatccccg agatggcgag cccgaggccg aagataatgc
     2341 cgcacccgag gcctgcgaag attttgaggg cagccatcag aagacgtgcc tcgtcaggaa
     2401 aacggtaagc atggccacca cgaagaacac cgccgtggcg accgccgagc gccaggagag
     2461 aagcgcgagc ccgcacaccc cgtggccgct ggtgcagcca gcgcccatcc tggtgccgaa
     2521 cccgaccagc agcccgccga ccaggatggc cggcggcgag gcgagcacgt cgaccggcgc
     2581 cggcccgccc tccaccagga tgtaggcgag ggcgcccagg ggtagtcctg ccacgaaggc
     2641 cgcacgccag cccctgtcgc cggccttcgg gttcaacagg gcgccgacga taccgcttat
     2701 tcccgctatg cgtccgttca gcaacagcgt caacgcgacg gcaagcccga tcaagagccc
     2761 acccagaagg ccgcttgccg gcgtgaagtc agccatccct gcctccgttc atcgtgttcg
     2821 ggccaacaaa tcgcgccagg atatcatgcc gcccacgaaa atcttcacag gttggccggg
     2881 atgagccgct cgaccacgac aacgtctcgc cactctccgt ccagcctggc gtgcttctcg
     2941 tgaaccccca cctcgcggaa gcccagggac cccagtaggt tcaggcttgg cccgttctcc
     3001 acgaaaacgc gggaggtgag cttccagaaa ccggcctgtt ccgcggtgga gatcaaagcc
     3061 tccatcgcca gggtgccagc ccccagcccc cttgcttcac cggctgtata gaccgagaac
     3121 tcggccacgc ccgagtagca ctcgcggggg ctataagcag aggcgttggc gaaggagatc
     3181 acccctcctt ccccctccac aacgacgatg ggatgcccgt cgtcgaacca acccatgatc
     3241 tccttgaccg cgcgcggcct ggtctcgaag gtcgcgatgc gctcctcgat gccctcgttg
     3301 tagatgcggg ctatggcctc cgcgtcgcca ggacccgccg ccctggaccg cagtcccgct
     3361 ggtcccttca tgccccgacg attatccaac gggcatcgat cctacgccga gaggtccaca
     3421 gcaacgggca aggaggtgcg acgccagttc gagagccggc ccgggctccg ctcgtacttc
     3481 tccaccagga gccgccgcgc cagttcgtct tcgtccgcgt cgcggacatc ccgggccacc
     3541 ccaccgagat cctccccagc gatgcggatc atgaccccgg ggtccctgcg aaggttcttc
     3601 acccagtccg agcggtcgcc gcccccggag agcatgtaca gtgtccccgt ctcgggcacc
     3661 agggagaacc agatctcgat ctcgtgtggg cgacccgaaa cacgaccggt agtggtcaga
     3721 taacagtact cctcaccagc gaacggccga agatcaggca caagaggccc caggtctcag
     3781 gctacaggtt tcaggtattc tcagagagca cccgtaacgc gagagtaacg ttcctgaagc
     3841 ctgaggcccg tagcctgtag ccctcatcct cgcaactcga cgcgtcgaat cttcccgctc
     3901 gtcgtctttg gcagctcctc gatagaccct atctggcgcg ggtacctgta cggggccgtc
     3961 tgctccttgc cgaggaggac catcaccctg tcctgcgccg cccaacggtc caccacgtca
     4021 cgcccgaagt tgaacctctc cggtgtgtcc cagccgaagt tcgcgtacgt ctcctcatag
     4081 tccccgatgt tggacattgg tacctcaccc gtccccaaag gtctctttcc gtaatgcggc
     4141 attctactag aagccgccca agctgagggt gtgccgcgtc ctgctggcgg gagtgttact
     4201 cgacgagcct ggtcgcggag aactccgacg tgttgcccgg gccggagttg ttggtgatta
     4261 tggtcgccgt tgcggtcacg agctctcccg tgttgacctg cgtattgaac gcgaaggaaa
     4321 ccctgccttc gctgttggtc accacgtttc tctcgcccag gaaggtcgtg ccctctctga
     4381 cgaggagcgg ggagatcggc ggtgggttcg agaagaactg gatggtgaag gtccttccgg
     4441 ggcggctgtt gagcctgccc tcgatccgcg tctcgcttgc cgaggtgagg acggggaagt
     4501 tctgaaggcc gttgggaccc gtgtcggggt cactggtgtc gttggcggtc acgccgtcgt
     4561 tgcccaggtc gatgctcagc cctggcggtg tcccgttgcc gaagatggag ttgcggagga
     4621 agctgttgcc cgtggcagcg tcgccgacga cgaccatccc ccggcttcga ttcagggcga
     4681 tcttgttggc cgccgcatcg accgtgccgc cgatagtgtt gcccgacgcg ccggtgatga
     4741 acacgccgtg caggttcccg aggttggata cgccgcccgc gtccgtaccg atcttgttgc
     4801 cctggaccac gttgttttcg gcgctggcgc caaatatgtg gacgccttca tcgccgttgg
     4861 ccgagatgac gttgcccgcc ccggtcgccg tccccccgat gaggttgcct ggtgtctcca
     4921 cccggactcc ggcctcggag ttgccgaagc ccgtcgtgcc gttcctgtcc gtgccgataa
     4981 agttgccctg gaccctgtta ccggtggcgc tgctcgtgcc cgagatgaag atgccgttgg
     5041 cgtcgttgcc agagatcaca ttccgtgcac cggcgaccgt gcccccgatc gtgacgcccg
     5101 aggagttccc gatgcgaatg ccgtctccca gattgccgaa gtccgaagcc ccgttactgt
     5161 tggtgcctat gacgttgcca agcaccacgt tgttcgtgga agaggtgccg acgatctcca
     5221 cgccgtgctc gccgttggcc gagatcgcgt tccccgcccc ggcgagcgtg tcgccgatct
     5281 cgaccctcgg ggtgctggag acgcgcaccc cgtctcgaga gttgcccacg ttcgaggagc
     5341 cgccgttgcg gttgaggccg atgaggttac ccacgatcct ggtgccctcg gcacctatgt
     5401 tggagacctc cacaccgtgc ctgtcgttgc ccgagatgat gtttagcgac ccgtccaacg
     5461 tgctcccgcc cacgagggtg tcggcggtgt tgtccacgaa caccccgctc agggtattac
     5521 caaggtcctc cgcggcgtcg gcgtcggttc ctatgtggtt gttggcgatg atgttgccag
     5581 gaccccctac ggaga
//
LOCUS       DBNGVD010000462         3483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2842981, whole genome shotgun sequence.
ACCESSION   DBNGVD010000462 DBNGVD010000000
VERSION     DBNGVD010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2842981"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16CM_12465"
     CDS             complement(<1..199)
                     /locus_tag="AC16CM_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140692.1"
                     /translation="MVEDGSKEEILRGVHPVLPSLALAFKRMLTTIERESGIGGMKWF
                     LLTVLDRRDGLSQGEFTQEYEL"
     gene            complement(573..773)
                     /locus_tag="AC16CM_12470"
     CDS             complement(573..773)
                     /locus_tag="AC16CM_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3140693.1"
                     /translation="MKTIEREDLKTLIDDGEDFVLLEVLSEQSYAREHLPGAVRFPGR
                     SEIPNLLPDIATTVVAYCSNFN"
     gene            complement(788..1255)
                     /locus_tag="AC16CM_12475"
     CDS             complement(788..1255)
                     /locus_tag="AC16CM_12475"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR3140694.1"
                     /translation="MTEKKATSGERDLSGAANLALKLGTLVHSGVYRATGGKLLGRMG
                     KSPILILNTVGRKTGKKRATPLLYAVDGEDFVIIASKGGTPSHPAWYLNLKANPDVSV
                     EVADREVRVRAEEVDGVVKERLWKRMAEMYPTYDDYQNKTKRNIPLLVLHPRD"
     gene            complement(1269..2033)
                     /locus_tag="AC16CM_12480"
     CDS             complement(1269..2033)
                     /locus_tag="AC16CM_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140695.1"
                     /translation="MGGFFQVAWAVAGRQTYKYFTNPAFLAPALFPLFFFVAFAGGLT
                     RIGDVPGFDYAAGYIAFQYVWALMQAVSMGGAFTGFSIAGDFENGFARRLMLAASNRY
                     GIILGYTMASLVRATMTGTLVTGIALLTGMPITGGYDVFGLIVLAVMANIAATLFGAG
                     VAMMLRTQQAGPIIRTPIFLVLFLAPVFVPLSLLTGWIEVAARLNPFTHILEAARGYL
                     AGDPVSVTSAFVISFALIIVLALWAVYGLRRAERAG"
     gene            complement(2035..2811)
                     /locus_tag="AC16CM_12485"
     CDS             complement(2035..2811)
                     /locus_tag="AC16CM_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140696.1"
                     /translation="MEGSTLLTQVLALARRSTVRTMRQPAVIISALLFPMMFFSINAS
                     GLAAASKIPGFPTDSYLDFAFAFPLIQASLFGAITAGSDLARDIESGFFDRLSLTPMR
                     PVALLGGMLAGVVALGLLQGIVFLAFGLLMGVDISSGFWGMLVIVLLTVLVALGFGGI
                     GAILAIRTGSVEAVESAFPLFFVAIFMSSINLPRNLIEQDWFRFIATINPISYLVEGI
                     RSLVITGWDPQALALGFGFAGAIVVVAIGGAAASLRTRVA"
     gene            complement(2811..>3483)
                     /locus_tag="AC16CM_12490"
     CDS             complement(2811..>3483)
                     /locus_tag="AC16CM_12490"
                     /inference="COORDINATES: protein motif:HMM:NF025109.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3140697.1"
                     /translation="QGALHGLSRKERRWRGQELLERVGLEEAADRKVRGYSGGMKRRL
                     DLALALVHGPRILFLDEPTTGLDPQSRSALWAEVSRLAREDGVTVFLTTQYLEEADVL
                     ADRVGIIDHGKIVAEDTPEALKAEIGRPSVEATPVESGDRDAVARILLGFGDEIPAQP
                     GAVAVRLSGGAAGLADVVRALDAEDVRVTNLRLDEPSLDDVFLAKTGRSLEGAGEDGE
                     MDEAQ"
BASE COUNT          632 a         1116 c         1111 g          624 t
ORIGIN      
        1 ccaactcgta ttcctgcgtg aactcaccct ggctcaaccc atccctcctg tccagcaccg
       61 tgagcaagaa ccacttcatg cccccgatcc ctgactcgcg ctctatggtc gtcaacatcc
      121 tcttgaacgc cagcgcgagc gaaggcagca ccggatgaac gccccgcaag atctcttctt
      181 tgctcccgtc ctctaccatc gtccatcctc cggttgcctt gctgtcgtgc gaagtctacg
      241 cccgatcttt gcagtttcaa cctttgcaag tgaaacaatt gtgaggatgt tcacgcgtca
      301 tcgaagatcg atcatcggag accctgagga tcgttattgt ggggcccgtg agggtctggt
      361 ctagtcgtgg gttcccgcat tcacgggagg gacgttatac atgtaacgcg tatagggaca
      421 cgtcgtatgc cgtggggtgg ctctcttgcc gcctaagccc tgctttcggt agggtagccg
      481 gcttcgatcc agtcctgttt gccgccctcg tactcgtagg cgttctcgta acccatggcc
      541 gtcagctcac ggacgacccg cgtcgaggag tgtcagttga agttcgagca gtaggcgacc
      601 acggtggtcg ctatgtcggg gagaaggttg gggatctcgg acctgccggg gaagcggacc
      661 gcgccgggca ggtgctcccg cgcataggat tgctccgaca gcacctccag caggacgaag
      721 tcttcgccgt cgtcgatcag ggttttcagg tcttcgcgct cgatggtctt caaagtgccc
      781 ctcccggcta atctctcggg tgcaatacaa gaagcgggat attacgcttc gtcttgttct
      841 ggtaatcgtc gtaggtcggg tacatctctg ccatcctttt ccagagacgt tccttcacta
      901 cgccgtcaac ttcctctgcc cttacccgta cctcgcggtc cgcaacctcg acgctcacgt
      961 cggggtttgc cttcaggttc aggtaccagg cgggatggga cggtgtgcct cccttggagg
     1021 cgatgatcac gaagtcctcc ccgtcgacgg cgtagagaag cggggtggca cgcttcttgc
     1081 cggtcttccg tccgacggtg ttcagtataa ggatagggct ctttcccata cgcccgagca
     1141 gcttaccgcc ggtcgcgcga tagacccccg agtgaacgag ggttccaagc ttcagcgcca
     1201 ggtttgccgc cccggagaga tccctctccc ctgatgttgc cttcttctct gtgatgactg
     1261 gctcctttct atccggctcg ctccgcgcgg cggagcccgt agacggccca gagggccagc
     1321 acgattatga gggcgaagct aatgacgaac gcggaggtta cgctcaccgg gtcgccggcc
     1381 aggtagccgc gggcggcctc cagaatatga gtaaacgggt tcaggcgggc cgcgacctcg
     1441 atccagcccg tcagcaggct cagcgggacg aagaccggtg ccaggaacag aaccaggaag
     1501 atcggagtcc ttattatggg tccggcctgc tgggtgcgga gcatcatcgc aacgccggcc
     1561 ccgaagaggg tagccgcgat gttcgccatc acggcaagaa ctatcaggcc gaagacgtca
     1621 tagccgcccg taataggcat cccggtgagg agcgcgatcc cggttaccag cgtcccggtc
     1681 atggtggcgc gcaccagcga ggccatggtg tatccgagga tgatcccgta ccggttggag
     1741 gccgcgagca tgagcctgcg ggcgaacccg ttctcgaagt cgccggcgat ggagaacccg
     1801 gtgaaggcac cgcccatcga gacggcctgc atcagggccc acacgtactg gaaggcgatg
     1861 tagccggccg cataatcgaa gccgggcacg tccccgatcc tggtcaggcc accggcgaag
     1921 gcgacgaaga agaacagcgg aaaaagcgcc ggcgcaagga acgccgggtt ggtgaagtac
     1981 ttgtaggtct gtcgaccggc cacggcccac gccacctgga agaacccgcc catcctaggc
     2041 caccctcgtc ctgagcgagg ccgcggcccc gccgatggcg accacgacga tagccccggc
     2101 gaagccgaag ccgagggcca gggcttgagg atcccagcct gttatgacca ggctccttat
     2161 gccctctacg aggtatgaga tcgggttgat ggttgcgatg aagcggaacc agtcctgttc
     2221 gatcaggtta cggggcaggt tgatcgagga catgaagatg gccacgaaga acagcgggaa
     2281 ggcgctctcg accgcctcta cggaacccgt cctgatcgcc agtatggccc ctatcccgcc
     2341 gaagccgagc gcgacgagca ccgtgagcag cactatgacc agcatgcccc agaatccgct
     2401 gcttatatcc acgcccatga gcagcccgaa cgccaggaag acgatgcctt gcaggagccc
     2461 gagggccacc acgccggcta gcatcccgcc gagcagcgcc acgggacgca tgggggtgag
     2521 cgagagcctg tcgaagaagc cgctctctat gtcgcgggca aggtcggatc ccgcggtgat
     2581 cgcgccgaag agcgaggcct gtatgagcgg gaaggcgaac gcgaagtcca ggtaggagtc
     2641 ggtggggaaa ccgggaatct tcgaggcggc ggcgagccca ctcgcgttga tggagaagaa
     2701 catcatgggg aagagaaggg cggagatgat cacggctggc tgcctcatgg tacggacggt
     2761 ggagcgtcgg gcgagggcca ggacctgggt gaggagcgtg gagccctcca ctactgcgcc
     2821 tcgtccatct cgccgtcttc gccggcccct tcgagggaac gccccgtctt ggccaggaac
     2881 acatcgtcga ggctgggctc gtcgaggcgg aggttggtaa cgcgcacgtc ttccgcgtcc
     2941 agggcgcgca ccacgtcggc gaggcccgcg gccccgccgg agagcctgac ggcgactgcg
     3001 ccgggttggg cggggatctc atccccgaac cccaacagga tcctggcgac cgcgtcgcgg
     3061 tccccgcttt cgaccggcgt cgcctcgacg ctggggcgtc cgatctcggc tttgagcgcc
     3121 tcgggcgtat cctcggccac gatctttccg tggtcgatga tcccgaccct gtcggcgagc
     3181 acgtcggcct cttcgaggta ctgcgtggtc aggaagaccg ttacgccgtc ctccctggcg
     3241 agcctgctga cctcggccca cagcgcgctg cggctctgcg gatccagccc cgtggtcggc
     3301 tcgtcgagga agagtatccg cgggccgtgc acgagggcca gggcgaggtc gaggcggcgc
     3361 ttcataccgc cagagtagcc gcgcaccttc ctatcggcgg cctcttccag gccgacgcgc
     3421 tccagaagct cctgcccccg ccaccggcgc tccttgcgcg agagtccgtg caacgcgccc
     3481 tgc
//
LOCUS       DBNGVD010000463         1549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4301625, whole genome shotgun sequence.
ACCESSION   DBNGVD010000463 DBNGVD010000000
VERSION     DBNGVD010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4301625"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16CM_12495"
     CDS             complement(<1..593)
                     /locus_tag="AC16CM_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3140698.1"
                     /translation="MDNPRTFDVLIVGGGQAGIPLAYKLAAEGISVALAERRDLGGSC
                     VNFGCTPTKAVIASARVAHQARRAGEYGLEIPEVRVDFPAVLQRAREIVELKRGGLDR
                     GLEEADNPTLIRGHARFEGREDDGFRLRIGDEPVLAGQVVLDTGTRTLTPPIEGLDDV
                     DYIESGNWLEKTELPESLAVIGGSYIGLEMAQFYNRMG"
     gene            complement(634..1323)
                     /locus_tag="AC16CM_12500"
     CDS             complement(634..1323)
                     /locus_tag="AC16CM_12500"
                     /inference="COORDINATES: protein motif:HMM:NF039426.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HIR3140699.1"
                     /translation="MVLLLVFFVALFMIVFLFLLPILFSLQAVGSLFVYPRQLRAIFG
                     NKILRRNHALEHATIVVMMEREPGRRLNGFSTDDGFFVQGVRSLAEVESAAREAIRRL
                     HNGEKKLAVHRNCGTTIVAANLLAAVFFLVALGVGLYLGWPLYLLIVGSIILAFALRV
                     PLSLLLQRFVTTDADLSNAEVGWVEPANPADLKNGIFGLLLAASTVQVRVFHTDPEAV
                     EIIRDDGAIVR"
BASE COUNT          296 a          515 c          477 g          261 t
ORIGIN      
        1 cccatccggt tgtagaactg ggccatctcc agcccgatgt agctgccccc gatcacggcg
       61 aggctctcgg ggagctccgt cttctccagc cagtttcccg actcgatgta atcaacgtcg
      121 tccaggccct ctatgggcgg cgtcagcgtg cgcgtgccgg tatccaggac gacctgaccg
      181 gctagcacgg gctcgtcgcc gatgcgcagc ctgaagccat cgtcctcgcg gccctcgaag
      241 cgggcgtggc cacggatcag ggtcgggttg tcagcctctt cgaggcccct gtccagtccg
      301 ccgcgcttga gctccactat ctctctggcc ctctgcagca cggccgggaa gtcgacgcgc
      361 acctccggta tctcgagccc gtactcgccc gctcgacgcg cctgatgggc gacccttgcc
      421 gaggcgatca cggccttggt cggcgtgcat ccgaagttca cacaggagcc gccgaggtcc
      481 ctgcgctcgg ccagcgccac ggatatgcct tcggccgcaa gtttgtaagc gaggggtatg
      541 cctgcctgcc ctccgcccac tatgaggacg tcgaaagtac gggggttatc catgatcccc
      601 tttctagaaa ccaggaaagt tcagcgaaga tctctaacgg actatggcgc cgtcgtcgcg
      661 gatgatctcg accgcttcag ggtccgtatg gaagacgcgc acctgcacgg tggaggcggc
      721 gagcagaagt ccgaagatgc cgttcttcag gtcggcgggg tttgccggct cgacccatcc
      781 gacctcggcg ttcgacaggt ccgcgtcggt ggtgacgaac ctctgcagga gcaggcttag
      841 ggggacgcgc agcgcgaagg cgaggatgat gctgcccacg atcaggaggt aaaggggcca
      901 acccagatac agcccgacgc cgagcgcgac cagaaagaag accgcggcga gcaggttggc
      961 agccacgatg gtcgtgccgc agttgcggtg gacggcgagc ttcttctcgc cgttgtggag
     1021 cctccgtatg gcctccctcg cggcgctctc gacctcggcc agcgagcgca cgccctgaac
     1081 gaagaagccg tcgtcggtcg agaacccgtt gagacggcgg cctggctccc tctccatcat
     1141 caccacgatg gtcgcgtgct caagggcgtg gttgcggcgc aggatcttgt tcccgaagat
     1201 ggcccgcaac tgccgcggat agacgaacag gcttcctacc gcctgcagcg agaagagaat
     1261 gggcagcagg aagaggaaga cgatcatgaa aagcgcaacg aaaaaaacca gcaacaggac
     1321 cacggtctca cctttgtaca gggtctacgc ctcgtgagta tactctagcg gccaacctcc
     1381 ggcgtcggca aacacacaaa taccaacctg caacgtcacc cacctttgca cctgtcagac
     1441 gcaacggcga ccccaagccc gaaacctaga gcgccacctc tcctggctcc tccaggtcgt
     1501 agagtagcgc gagcgcagcg agggccacct tcctgtggcc ctcccggtc
//
LOCUS       DBNGVD010000464         3710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3754668, whole genome shotgun sequence.
ACCESSION   DBNGVD010000464 DBNGVD010000000
VERSION     DBNGVD010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3754668"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16CM_12505"
     CDS             <1..389
                     /locus_tag="AC16CM_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140700.1"
                     /translation="SSWPLYLAKGEIITAVDSKARRIDNYMPLTVTTREKSLILGGPD
                     EEPELYDLSRDPDERENTWATNGEGALLAEHALSFLEGVRTPEEYLAPRRLALQRFAP
                     NLLRGSAATPQNTQQGQIQNASKEAV"
     gene            386..1555
                     /locus_tag="AC16CM_12510"
     CDS             386..1555
                     /locus_tag="AC16CM_12510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3140701.1"
                     /translation="MSVEVDTVRTVEVPTRMVHGPGAIASLGEIVEELGVKRPMLVTD
                     AGLVAAGLAEKALEHLTEATVFEGVKPNPDVELIGRASEVYKEEGCDGLVALGGGSSM
                     DTAKGVGVEIVHGGSIVDYEYAKTPITRRIPPLVTVPTTAGTGSEVTLWAVITDHERK
                     IKFNAGGTALIGAHVALIDPALMVGLPTAITAATGMDALSHGIECYTCDYHQPFTDSF
                     ALTAIEYVARWLRAACQDGSNMEARTFMAHAATFGGMAYGTESAGAAHAMSQSAGGVH
                     DSPHGALTARVLGPVCEYNAPAAPERYARIAQALGADIRGLEPSEAALVGIEELYRLT
                     EDVGIPTMEELGFSEDEIPMLARIAYEDPQTVGNPREVTVADYEEIYHDAFARGK"
     gene            1555..3012
                     /locus_tag="AC16CM_12515"
     CDS             1555..3012
                     /locus_tag="AC16CM_12515"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3140702.1"
                     /translation="MYTKTKDYSMYVAGKWATGESEARMETTSPATGEPIGTVPRGTR
                     TDVERAIAAANGAWLGWAARSAFERAAAMIRIAEIIEERREDLAQTLTLDQGKPLAAE
                     AYDEVDELVAYFEMASADVTRLEGAMPPSVDADKRVMLYRVPRGVVGVISPWNWPYTM
                     PAELIAPALACGNAVVWVPAPSTSVCAVKLAECIVDAGLPPGLFNMITGQGPVVGDEV
                     ASNPGTQAVGFIGSIETGQKVAARAAGKALLLEMGGNGPMVILDDADIEAAAEESLTS
                     AFLGAGQSCTAGERFLVHERIYEAYLEELDRAIGRRIKLGDPFDPTTTLGPLNNEPTA
                     EKMDRHISDALDGGAELISGGTRAEGFPTDLYYQPTVLGNVSAGMRVSKEETFGPIVP
                     ATIIRDEEEAIRTINASPYGLLSAVFTRDLSRGLRFAESVRTGWVNVNASSNHWESHL
                     PFGGRAGSLSGVGRVGGRYAMETFTEFKTVILNLD"
     gene            3585..3710
                     /locus_tag="AC16CM_12520"
                     /pseudo
     CDS             3585..3710
                     /locus_tag="AC16CM_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          748 a         1203 c         1172 g          587 t
ORIGIN      
        1 tcagctcctg gccgctatac ctggccaagg gtgagatcat caccgccgtt gactccaagg
       61 cgcgcaggat agacaactac atgccgctaa ccgtgaccac cagggagaaa tccctgatcc
      121 tgggcgggcc agacgaggag cccgaactct acgacctcag ccgcgacccg gatgaacggg
      181 agaacacgtg ggccactaac ggtgaaggcg ctctgctcgc cgagcatgcc ctttctttcc
      241 tcgaaggcgt gcgcaccccg gaagagtacc tggctccccg ccgcctcgcc ctccagaggt
      301 tcgcaccaaa cctcttgcgg ggttccgccg cgacaccaca gaacacccaa caaggtcaga
      361 tacaaaacgc gagtaaggag gccgtatgag cgtcgaagtc gacacagtac gcaccgtcga
      421 agtccctacc cgcatggtac acgggcccgg ggccattgct agcttgggcg agatcgtgga
      481 ggagttgggc gtgaagcgcc cgatgctcgt aacagatgca ggcctggtcg ccgcgggtct
      541 tgcagagaaa gccctcgagc accttacgga ggccacagtc ttcgaagggg tcaagccgaa
      601 ccccgacgtg gaactcatcg gacgggcgag cgaggtctac aaggaggagg gctgcgacgg
      661 gctcgtggcc ctcggcggcg gcagttcgat ggacacggcc aagggagtag gcgtcgagat
      721 agtccacggc gggtccatag tcgactatga gtacgccaag acccctatca cgcgtcgcat
      781 ccccccgctc gtgacggtgc caacgacggc agggacaggc agcgaggtaa cgctctgggc
      841 cgtcatcacc gaccacgagc gcaagataaa gttcaacgcc ggtggtacgg ccctgatcgg
      901 cgcccacgtg gcgcttatag accctgcctt gatggtcggc ctgccgaccg cgatcacggc
      961 ggcgacgggg atggacgcgc tctcacacgg tatcgagtgc tacacctgcg actatcacca
     1021 gccctttacc gactctttcg ccctgacggc catagagtac gtggcgcggt ggctgcgggc
     1081 cgcctgccag gacggctcga acatggaggc ccgcacgttc atggcgcacg cggccacgtt
     1141 cggggggatg gcctacggca cggagagcgc aggggccgca cacgctatga gccagtcggc
     1201 tgggggtgtc cacgacagcc ctcacggcgc cctgacggcg cgcgtcctcg gtccggtctg
     1261 cgagtacaac gcccccgccg cgcccgagcg ctacgcccgc atcgcgcagg cgctgggggc
     1321 cgacattagg gggctcgaac catccgaggc cgccctagtc ggcatcgagg aactctaccg
     1381 tctgaccgag gatgtaggta ttccgacgat ggaggagttg ggcttctcgg aggatgagat
     1441 cccgatgctg gcccggatcg cctacgaaga cccgcagacg gtcggaaacc cccgcgaggt
     1501 cacggtcgca gactacgagg agatctacca cgacgccttc gcaaggggga aataatgtac
     1561 acaaagacga aagactactc gatgtacgtc gcgggcaagt gggccaccgg cgagtccgaa
     1621 gcccgcatgg agacaacgag cccggcgacg ggagagccga tcggaaccgt accacgagga
     1681 acccgcacgg acgtagagcg ggcgatagcc gccgcgaacg gggcctggtt ggggtgggct
     1741 gcgcgctccg cgttcgagcg tgccgcagcg atgatccgga tcgccgagat catcgaggaa
     1801 cgccgcgagg acctggcgca gacgttgact ttagatcagg gcaagccgct tgcggccgag
     1861 gcctacgacg aggtcgatga gctcgtggcc tacttcgaga tggcaagcgc cgacgtaacg
     1921 cggctcgagg gggccatgcc accctcggta gacgccgaca agcgcgtcat gctctacagg
     1981 gttccgcgcg gggtggtcgg cgttatctcc ccgtggaact ggccctacac gatgcccgcg
     2041 gagctgatcg cgccggcgct agcttgtggc aacgcggtcg tctgggtgcc cgctccctcg
     2101 acatccgtct gcgccgtgaa gctcgccgag tgcatcgtcg acgccggact gcccccgggg
     2161 ctcttcaaca tgatcacagg ccaggggccc gtggtagggg atgaggtggc gtccaaccca
     2221 ggcacccagg ccgtcggttt tatcggctcc atcgagaccg ggcagaaggt ggccgcgcgc
     2281 gccgccggca aggcgctcct cctcgagatg gggggcaacg gtccgatggt gatcctcgat
     2341 gacgccgaca tcgaagccgc cgccgaggag agcctgacct cagccttcct cggcgctggc
     2401 cagagttgca ccgccgggga gcgcttcctg gtacacgaga gaatctacga ggcctacctc
     2461 gaagaactcg accgtgcgat cgggcgcagg atcaagctcg gcgacccatt cgaccccacg
     2521 accacgctcg gccccctcaa caacgaaccg acggccgaga agatggaccg gcacatctcc
     2581 gatgcgctgg atggcggcgc ggagctgatc tccggcggga cccgcgccga aggattcccc
     2641 acggacctct actaccaacc aaccgtgttg ggaaatgtca gtgcggggat gcgcgtctcg
     2701 aaggaggaaa ccttcggccc catagtcccc gcgaccatca tccgcgacga ggaagaggcc
     2761 atacggacga tcaacgcctc gccctacggc ctgctctccg ccgttttcac cagggacctg
     2821 agccgggggc tacgcttcgc cgagtccgtg agaaccgggt gggtcaacgt aaatgcctcc
     2881 tcgaaccact gggagagcca ccttcctttc ggcggaaggg ccgggtccct ctccggagtc
     2941 gggcgcgtcg ggggacgcta cgcgatggag actttcaccg agttcaaaac cgtcatcctg
     3001 aacctggact gacttgcgaa ggcgcgtccc caacgcggcg gcccaaaccc gccccgacac
     3061 tagcctgatg caatttagag cttgcgccaa taggtgggta gcatatgtgt caagagccag
     3121 caagtaggct gggtaatctc tccgagaggt gtcatgtatg aaaagttgct agcctattgg
     3181 cgcggcctct tatacggggc gacgacagga cggaccaatg gcacacaacc caggcattcg
     3241 gggcgtgctt cgggagtcgc gttgcacctg atcgcgacgc cctacggtcc ggctcactga
     3301 ctaacaagca tactatttag ttttgctcgc gcgaggttgg cctctgagtg cctccgcgga
     3361 acctccgaag gttccaagaa taccgattag cagaagttca actaccgaag acgctccgcc
     3421 tccacaacct ctgtacgcta tcctgaccct ctcgtagaga accggcgcgg ccagatccgc
     3481 accttttctt taggtcaccg ccgattctac tcgtcgggta ccgttttccg cgacgaggat
     3541 ctggaagccc acggtagtgg ggtttgtact gcggacggat gacaaaccag atcacctggt
     3601 ccaagcccaa cccgccaccg aaccttctcc acacagcctt cacccactca catgagacat
     3661 tgctgtgggc gagcaggagc aagtactcca ggcacacctt caactacgac
//
LOCUS       DBNGVD010000465         3803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2297448, whole genome shotgun sequence.
ACCESSION   DBNGVD010000465 DBNGVD010000000
VERSION     DBNGVD010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2297448"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..872)
                     /locus_tag="AC16CM_12525"
     CDS             complement(234..872)
                     /locus_tag="AC16CM_12525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140703.1"
                     /translation="MRLVVFGATGGTGGRIVEQALATGHGVTAVVRDPERLQLRHERL
                     RVVRADVFRSAEIGPLMVGADAVLSALGPHTYRAETTVCSRSIRNILGAMREVRVGRL
                     VCISAAPVGSAGKDDTFLYRFVARPLVRAVFKGVYKDLAEMEAEVRHSGTDWTIFRPP
                     RLTDGPRTGRYRLGHDQNVVGASTISRADLADAMLGSLEDPGATRRTVGIGY"
     gene            complement(885..1325)
                     /locus_tag="AC16CM_12530"
     CDS             complement(885..1325)
                     /locus_tag="AC16CM_12530"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3140704.1"
                     /translation="MGQGEENGVVLRRFVDEVINTGEVGLVPEFVGESFVDHDPPPGA
                     SPDISGLGKAFARVRSSFPDFHATVEDLVAEGDMVAYRWTFRGTHLGDFGGMTPTGRV
                     AVWSVIGIARFAEGKMVERWQRLDTAGLLRQLDDSGVVTQPTGS"
     gene            1407..2012
                     /locus_tag="AC16CM_12535"
     CDS             1407..2012
                     /locus_tag="AC16CM_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140705.1"
                     /translation="MDTVGTRERMVEAAERVMRERGLARSTTREIAREAGFSEGALYK
                     HFEGKEDLFLHVLAERLPSFVALAKELPGRAGVGTVEDTLGEVARRSLPFFAEGVPII
                     ASIFSEPALLSRHAEEIRRRGGGPQRANEAVAAYLRAEQELGRVREDADPEAAAGMLL
                     GACFQRAFLRQFLAEEIDPAEEEGFARKVVVTLMQSLSPGS"
     gene            complement(2020..2397)
                     /locus_tag="AC16CM_12540"
     CDS             complement(2020..2397)
                     /locus_tag="AC16CM_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140706.1"
                     /translation="MVLSAIPPARSFQNLFVGPFGLAIQEGTAWTQLPIAIFILLSPV
                     ACLVLFLISLRQTLSPSLRQRCAVATFVLPSVALALYALYESGIPPEMNIRIDLLLIY
                     PALALDFLFWPALLVRYLVFRRA"
     gene            2683..>3803
                     /gene="hydA"
                     /locus_tag="AC16CM_12545"
     CDS             2683..>3803
                     /gene="hydA"
                     /locus_tag="AC16CM_12545"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR3140707.1"
                     /translation="MGELLFSGGTVVTAEGSYKADVLVDGDKIVAVATGLEADGAEVV
                     DVTGKLLMPGFIDAHTHMDMPFGGTVTADDWATGTEAAVAGGTTMIIDFALQEAGGTL
                     AEAYDAWTEKARGKAVIDYSFHVAITDLRDDIKDELPDLAAKGVASVKIFMAYKGTPL
                     YTEDEDLFEALQLSKEAGVLVMVHAENGDAISKLQQQALARGDTAPRFHALTRPEAVE
                     AEATNRAIRLAEVAGAPILVVHVSCAGALEAIHLAHERGQTVYGETCPQYFQFEYEDL
                     AREGFEGAKFVCSPPLRDPGNRPALWNGLKFGDLQIFGSDHCSFNYEGQKELGKEDFT
                     LIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQA"
BASE COUNT          713 a         1254 c         1214 g          622 t
ORIGIN      
        1 ggttacgctt gggccgctcg tcgggcggct gctggcgcgc gagatcctga cgggcgaggt
       61 tgaccctctt atcgcgccgt tccggccgga tcgcttcgcg taaccccgca cgcaaccctc
      121 cactgtggtc ctcggttaga caaaccaaaa agcgctggtc atccgtccgc aaccaggccg
      181 attagtccag tggtcttact gatgcctgta gcctgatgcc tgtggcctcc aattcagtaa
      241 cctatgccga ccgtcctacg agtcgcacca gggtcttcca ggctaccgag catggcgtcg
      301 gcgaggtccg cgcgcgagat ggtgctggcg ccaaccacgt tctggtcgtg gcccagccgg
      361 taacggcctg tgcgcggccc gtctgtgagc cgcgggggac ggaatatcgt ccaatcggta
      421 ccgctatgcc gcacctcggc ctccatctcg gccaggtctt tatacacccc tttgaagacc
      481 gcccggacca ggggcctcgc cacaaacctg tataggaacg tgtcgtcctt gccggcagac
      541 cccaccggcg cggcgctgat acagactaac ctccccaccc tgacctcccg catcgccccg
      601 aggatgttcc ggatggaccg ggagcacacc gtcgtctcgg ccctgtaggt gtggggaccg
      661 agcgccgaca aaacggcgtc ggccccgacc atcaggggcc ctatctccgc gctcctgaac
      721 acgtcggcgc ggacgacccg tagccgctcg tgacgcagct gcagccgctc cgggtccctc
      781 accacggccg tcacgccatg gcctgtggcg agcgcctgct ccacgatacg cccgccggtt
      841 ccacccgtcg ccccgaacac caccagcctc ataaccatct cccttcagct acccgttggc
      901 tgcgttacca cacccgaatc gtcgagttgc ctcaacagac ccgccgtgtc cagtcgctgc
      961 cagcgctcga ccatcttgcc ctccgcgaag cgcgcgatgc cgatcaccga ccacacggcc
     1021 acccggccgg tgggggtcat gcccccgaag tctcccaggt gggtgccacg gaacgtccag
     1081 cggtacgcca ccatgtcccc ttcagccacc agatcctcga ccgtggcatg aaaatcgggg
     1141 aaggatgagc gcaccctcgc gaaagccttt ccgaggcccg agatgtcggg cgaagcaccc
     1201 ggaggggggt cgtgatccac aaagctctca ccaacgaact cgggcaccag acctacctca
     1261 ccagtgttga tgacctcatc gacgaaccgc cgcaaaacga ccccgttttc ttctccttgc
     1321 cccatacccg cctcctgtgt gggtgagcgc ttacttacct gggtgatggt atggtaagtg
     1381 ggtgctcgct cactgtcaag aggaggatgg atactgtggg gactcgggag cgcatggtcg
     1441 aggctgcgga gagggtgatg cgcgagcggg ggttggccag gagcacgacc agggagatcg
     1501 cgcgcgaggc cggtttctcg gagggggcgc tctacaagca cttcgagggc aaggaggacc
     1561 tgtttctgca cgtgttggcg gagcgtctgc cttcctttgt ggcgctggcc aaggagcttc
     1621 ctggccgggc cggggttggc acggtcgagg acacgctcgg ggaggtggcg aggaggtcgc
     1681 ttcccttctt tgccgagggc gtgcccataa tcgcctccat cttctcggag cctgccctgc
     1741 tctcccgcca cgcggaggag atccggcgga ggggcggagg gccgcagagg gcgaacgagg
     1801 ccgtggccgc ctatttgcgg gccgagcagg agcttggacg ggtgcgagag gacgctgacc
     1861 ccgaggccgc ggcggggatg ctactggggg cgtgcttcca gcgggccttt ctccgccagt
     1921 tcctggccga ggagattgat cccgcggagg aggaggggtt cgccaggaag gtcgtggtga
     1981 ccctcatgca aagcttgtcg cccgggagtt aggagaatct caagcgcggc gaaagaccag
     2041 gtaccgcacc agaagcgcgg gccagaacag gaagtcgagg gcgagcgcgg ggtagatgag
     2101 cagcaggtct atgcgtatgt tcatctccgg cggtatcccc gactcgtaga gggcgtacag
     2161 ggccagggcc acggacggca gtacgaaggt cgccacggcg cacctttgtc tcagcgacgg
     2221 cgacagggtc tggcgcagtg agatcaggaa aaggaccagg caagccaccg gggacagaag
     2281 gatgaagatc gcgatcggca actgcgtcca tgccgtaccc tcctggatcg ccagcccgaa
     2341 cggtcccacg aagagatttt ggaaggaccg tgccggtggg atggccgaca gaaccataag
     2401 attcaccccc gatgcaatac acgtttgttc cataaaggag cacctatccc cttcctgatc
     2461 ggtacctgtc gaacagttta caaccggcct ccgcggcgcc tatactgacc ttccaggaaa
     2521 gggaggcgag tacgcaggat cagcgttcgc acagcaggcg tttctcctcg agggtgggat
     2581 cccgccaccc tcgtctagaa ccaggccggg taccgtgctt tatagtgtcg acgaaggact
     2641 ctccgggggg cgcccacgag cttgtggaaa ggagcaggag cattgggaga attgctattt
     2701 agcgggggaa ctgtcgtaac cgcggagggc agctacaagg ccgacgtgct cgtcgacggc
     2761 gacaagatag tggccgtcgc caccgggctc gaggcagacg gtgccgaggt cgtcgacgtc
     2821 acgggcaagc tcctgatgcc cggcttcata gacgcccaca cccacatgga catgcccttc
     2881 gggggcaccg tgacggccga cgactgggcg acgggcaccg aggccgcggt ggccggcggg
     2941 acgaccatga tcatcgactt cgccctgcag gaggctggcg gcaccctggc ggaggcgtac
     3001 gacgcgtgga ccgagaaagc acgcggcaag gccgtcatcg actacagctt ccacgtggcc
     3061 ataaccgatc tgcgcgacga tataaaagac gagttgcccg acctcgcggc gaagggtgta
     3121 gcctccgtca agatcttcat ggcctacaag ggaacgccgc tctacaccga ggacgaggac
     3181 ctcttcgagg cactccagct ctcgaaagag gcgggtgtcc tggttatggt tcacgccgag
     3241 aacggcgacg ccatctccaa gctgcagcag caggcgctcg cccgcggcga cacggccccc
     3301 cgcttccatg ccctcacccg ccccgaggcc gtagaggccg aggcgaccaa cagggccata
     3361 aggctggccg aggttgccgg ggccccgata ctcgtggtcc acgtctcctg cgccggcgcc
     3421 ctcgaagcca tccatctcgc ccacgagcgc ggccagaccg tctacggcga gacctgccca
     3481 cagtacttcc agttcgagta cgaggatctg gccagggaag gcttcgaggg ggccaagttc
     3541 gtttgctccc cgccgcttcg cgaccccggc aacaggcccg cgctctggaa cgggctcaag
     3601 ttcggggacc ttcagatctt cggctcagac cactgctcgt tcaactacga gggacagaag
     3661 gagcttggca aggaagactt caccctcatc cccaacggcc tacccggcgc cgaagaacgc
     3721 gccatgacgc tctggacgct cggcgtacgc gagggcaagc tctccgaaaa ccagttcgtc
     3781 gccgtcctct ccaccaacca ggc
//
LOCUS       DBNGVD010000466         4116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_840700, whole genome shotgun sequence.
ACCESSION   DBNGVD010000466 DBNGVD010000000
VERSION     DBNGVD010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_840700"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..703
                     /locus_tag="AC16CM_12550"
     CDS             98..703
                     /locus_tag="AC16CM_12550"
                     /inference="COORDINATES: protein motif:HMM:NF025605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140708.1"
                     /translation="MAGESDRREQILEAAFEEFSAHGFKGATIKGIARAAELQSPALI
                     YWYFPDKEALFREVLETRIPVLRAVRDPASLLDLPPEVVLPTIARRYLATFDSRATQR
                     LARLLVGEAMRRPEVADVFGNAVLGRVLGFLKSYMSRQIELGRLGPHDVRASARAFIG
                     MLVPQAGGKVFFSAIREDGLTDEEHVEAAVTIFLRGLGKED"
     gene            706..1557
                     /locus_tag="AC16CM_12555"
     CDS             706..1557
                     /locus_tag="AC16CM_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140709.1"
                     /translation="MNAIEVSGLAKKYGRFEALRGVDLSVGKGEVFGLVGPNGAGKTT
                     LIKGLVGALRPSEGGVRVMGLNPLRDRAELRQRIGYMPQGPALYDDLSARGNVEFFGA
                     AHRTGNLKKRVEEVLEFTDLSERAGDAVHTFSGGMKRRVSLACALVHEPEVIFLDEPT
                     AAVDPQLRSRFWKTFRELARGGTTLFISTHLMDEAVLCDRIAILRKGGVIATDTPGNI
                     IERGSTRLVVKSGGIGKEETIGGHPEDLAAALRPYGLGEDVTAVDVRTDTLEEVVLSL
                     IEKDEVG"
     gene            1554..2294
                     /locus_tag="AC16CM_12560"
     CDS             1554..2294
                     /locus_tag="AC16CM_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140710.1"
                     /translation="MMRRTFVIAGRVVRQLIRNPRLLVLSVAAPLVIVYFLKIFFDTL
                     PPTFDVARYAVPIAAFVVHFLAFLLCAIALVQERTMGTMERMFINGFRRTEIIAGYVL
                     GFLGLATFQAVAGLAEAIWLFDLDYEGDTLAMLFVVVWLLAIASVMLGIFVSTFARHE
                     GQVFPFVPLIILPSVFLSGLLVDVDELPTWADWLGHVFPLFWANDVIQDLIESGWTFD
                     DIRSSLAVLAGYSVVLLLLASRTLREVE"
     gene            2318..>4116
                     /locus_tag="AC16CM_12565"
     CDS             2318..>4116
                     /locus_tag="AC16CM_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3140711.1"
                     /translation="MERVDNPELTIPSSEFVVPFAALDRDALPVAGGKAANLGELVRA
                     GFPVPPGFCVTTAAYEVVASDAGLGPMLEALAATDAGDTERLTGLAAEVRSCLAGADI
                     PDVLRMAIEEAYGELGDGSAVAVRSSATAEDLPGASFAGQQDTYLNVVGVDPVVDAVQ
                     RCWASLWTDRAVSYRATNGIDQAGVRLAVVVQRMVSASVAGVLFSANPVTGKRHQAVI
                     DASPGLGEAVVSGAVNPDHFVVNTATGEIVERHLGDKRVAVVAGSEGGVERVELGADE
                     HGACLSDAQILALAGLGARVEDHYGAPQDTEWAIDSEDRLWLLQARPITTLFPLPDDA
                     PADDEDLRVYFSFNVAQGVYGPLTPMGIQAFRLITSSVATFAGHPPRNPYEGAVFFVE
                     SAFRMFLDVTPILRNKHGLQLFEGFMTNMETRTVPIVRRLATDPRLANVPTEWWPILR
                     TLLLVLARGRAPLHVAEALARPEGARARAARAVARLREADGAPTGASGHLVAAERLLR
                     DGPPEILRNVPPVFVTGLLVNALAGKLLGNLATFDERRVAMRALPHNPTTEMDLALWE
                     LAKEARADPEIARMLGETPPERLAEDYHDGSLPP"
BASE COUNT          673 a         1242 c         1435 g          766 t
ORIGIN      
        1 tgctcccttt acccatattt cgtgtcggta ttacctcttg actgaacgtt ctatcaagtc
       61 tatagttatt gattaaacga tcaataaagg ggcttcgttg gctggggagt cagacaggcg
      121 ggagcagata ctggaggcgg ccttcgagga attctctgcg catgggttca agggggccac
      181 gatcaagggc attgcgcggg cggctgagtt gcagtctccg gcgcttatct actggtattt
      241 cccggacaag gaggctcttt tcagggaggt tctggagacc aggatcccgg tgttgcgcgc
      301 ggtgagggac cctgcttccc tgctcgatct accgccggag gtggtgttgc cgaccatcgc
      361 caggagatac ctggcgacgt ttgacagtcg ggccacgcag aggctggcgc ggttgctcgt
      421 cggggaggcc atgcggcgtc cggaggtcgc cgacgtgttc gggaacgcgg tgctagggcg
      481 ggtgctcggg tttttgaaga gctacatgtc gcggcagatc gagctgggca ggctcgggcc
      541 gcacgacgtc agggcaagcg ccagggcgtt tatcgggatg ctcgtgccgc aggccggggg
      601 caaggtgttc ttctcggcca tacgggagga tggcctgacg gacgaggagc atgtggaagc
      661 ggcggtcact attttcctgc ggggcctggg aaaggaggac tagagatgaa cgccatagag
      721 gtctctgggc tggccaaaaa gtatggccgg ttcgaggcgt tgcggggcgt ggacctgtcg
      781 gtgggcaagg gtgaggtgtt cggcctcgtc gggcccaacg gggcgggcaa gaccaccctc
      841 atcaaggggc tcgtcggggc gttgcgtcct tccgagggtg gggtgcgcgt gatggggttg
      901 aacccgctca gggacagggc agagctcagg cagagaatcg gttacatgcc ccagggcccc
      961 gcgctctacg acgacctctc cgccaggggc aacgtcgagt tcttcggcgc cgcccatcgg
     1021 accgggaacc tcaagaagag ggtggaggaa gtcctcgagt tcacggatct atcggagcgc
     1081 gccggggacg ccgtacacac cttctccggc gggatgaagc ggcgggtctc gctggcctgc
     1141 gcgctggtcc acgaacccga ggtcatcttt ctcgacgagc ccacggcggc agtggacccg
     1201 cagctcagga gcagattctg gaagaccttt cgggagctgg ccagaggcgg gacgacgttg
     1261 ttcatcagca cgcacctcat ggacgaggcg gtgctgtgcg accgcatcgc gatcctccgc
     1321 aagggtggcg tcatcgccac ggacaccccg ggcaacatca tcgagcgggg ctcgacgcgg
     1381 ctcgtggtga agagtggagg catagggaaa gaggaaacca tcggtgggca ccccgaggac
     1441 ctggcggcgg cgcttcgccc gtacgggctg ggggaggacg taaccgctgt ggacgtgcgg
     1501 accgacacgc tggaggaggt cgtcctctcg ctcatagaga aagatgaggt gggatgatgc
     1561 gccggacgtt cgtcatcgcc gggagggtag ttaggcagct catcaggaac ccgaggctgc
     1621 tagtgctctc ggtggccgct ccgcttgtga tcgtctactt tctcaagata ttcttcgaca
     1681 ccctgccccc caccttcgac gtggcgcgct acgccgtgcc catcgccgcc ttcgtggtcc
     1741 actttctcgc cttcttgctg tgcgccatcg cgctcgtgca ggagaggacc atgggcacca
     1801 tggagcggat gttcatcaac gggttcagaa gaacggagat aatagccggg tacgtgcttg
     1861 gttttctggg cctggccacc ttccaggccg tcgccgggct ggctgaggcc atctggctct
     1921 ttgacctcga ctacgagggc gacacgctgg cgatgctctt cgtggtggtg tggcttctgg
     1981 ccatagcctc cgtgatgctt ggcatcttcg tctccacctt cgcccgccac gagggccagg
     2041 tcttcccctt cgtgcctctg atcatactcc cctcggtctt tctctcgggg ttgctggtgg
     2101 acgtggacga gctgccgacc tgggcggact ggcttgggca cgtgttcccg ctgttctggg
     2161 cgaacgacgt gatccaggac ctcattgaat cgggctggac ctttgacgac ataaggtcaa
     2221 gcctggccgt gctcgccggg tacagcgtgg tcttgctctt gctggcctcc cgcaccttga
     2281 gagaagtcga gtaaagcgcc gggaactgga ggaaaccatg gagagggtgg ataaccccga
     2341 actcacgatt ccaagctccg aattcgtagt tccattcgcc gccctggaca gggacgcgct
     2401 gccggtggcc ggtgggaagg ctgcgaacct cggggagctg gttcgtgcgg ggtttcccgt
     2461 gccaccgggg ttttgcgtaa cgacggcggc gtacgaggtg gtggcgtcag atgccgggtt
     2521 gggacccatg cttgaagctc tggccgcgac ggatgccggg gacacggagc gtctgaccgg
     2581 gctcgcggcg gaggtgagat cctgcctcgc gggggccgac attcccgacg ttctcaggat
     2641 ggccatcgag gaggcctacg gcgagttggg tgacggttcg gccgtggccg tgcgctcttc
     2701 ggccacggcc gaggacctgc ccggggccag ctttgcggga cagcaggaca cgtacttgaa
     2761 cgtggtcggc gtcgacccgg tcgtcgacgc ggtgcagcgc tgctgggctt cgctgtggac
     2821 ggatcgggcc gtcagttacc gggccacgaa cggcatcgac caggcaggcg tgcgactcgc
     2881 cgtcgtcgtg cagcggatgg tcagcgcttc tgtcgccggc gtcctgttta gcgccaaccc
     2941 ggtaacaggg aagcgtcatc aggctgtcat agatgcaagc ccgggcctcg gcgaggccgt
     3001 cgtttctgga gcggtcaacc ccgatcattt cgtagtgaac accgcaaccg gggagatcgt
     3061 cgagcgacac ctgggagaca agcgcgtggc cgtggtggca ggttcggaag gcggggtcga
     3121 gcgggtcgag ctcggcgctg acgagcacgg ggcctgtctc tcggacgccc agatccttgc
     3181 cctggcagga cttggcgcgc gggtcgagga tcactacggc gctccccagg acacggagtg
     3241 ggccatcgac agcgaagatc ggctctggct gttgcaggcg cgtcccatca cgaccctctt
     3301 cccgttgccg gacgatgccc cggccgacga cgaggacctg cgtgtgtact tctccttcaa
     3361 cgtcgcccag ggcgtctacg gcccgctaac ccccatgggc attcaggcgt tccgcctcat
     3421 aacctcgtct gtggccacgt tcgcgggcca ccccccacgg aatccatacg aaggagcggt
     3481 gttcttcgtg gagtcagcct tccgcatgtt tctggatgta acgcccatcc ttaggaacaa
     3541 gcatgggctc cagctcttcg aggggttcat gacgaacatg gagacgagga cggtccccat
     3601 cgtcaggcgg ctggccaccg atccccgcct ggccaacgtg ccgacggagt ggtggccgat
     3661 cctgcgtacc ctgctcctgg tgttggcgcg ggggcgcgcc cccctacacg tcgccgaggc
     3721 gctcgcgcgt ccggaggggg cgagggctcg cgccgcccgc gccgtggcaa ggttacgcga
     3781 ggcggacggg gcacctacag gggcctccgg gcacctcgtt gccgccgagc ggctgctgcg
     3841 cgacggccct cccgagattc tacgcaacgt gcccccggtc tttgtcacag gcctcctcgt
     3901 caacgcgctc gccggcaagc tgctcgggaa ccttgccact ttcgacgaaa ggcgggtggc
     3961 gatgcgcgcg ctcccgcaca acccgacgac ggagatggac ctggcgctct gggaactcgc
     4021 caaagaggcg cgtgcagacc cggagatcgc caggatgttg ggtgaaaccc cgccggagcg
     4081 cctggcagaa gactatcacg acggctccct gccccc
//
LOCUS       DBNGVD010000467         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_111600, whole genome shotgun sequence.
ACCESSION   DBNGVD010000467 DBNGVD010000000
VERSION     DBNGVD010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_111600"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="AC16CM_12570"
     CDS             <1..511
                     /locus_tag="AC16CM_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3140712.1"
                     /translation="AKVIVGDDGSKSAELAAGLAADLGGLYAAEITLIRAHTNPPEPI
                     GGWSSKDRSGLDLMRSREADAIAGRAREIEKVTGTRPSIRVLDTDAVLAMMLVAEKGE
                     ERRTLIAVGSRGLGVIGRVRLGSVSTKILRIARGPVLIHAGSLDEIDAHEHGKAASVV
                     GRQARVPGG"
     gene            733..1341
                     /locus_tag="AC16CM_12575"
     CDS             733..1341
                     /locus_tag="AC16CM_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIR3140713.1"
                     /translation="MTEHDRGKLVTLSGDLPIWERVFTVNPLVVVGTREREGGYDLAP
                     KHRAFPLGWDNYFGFVCTPRHGTYDNVRREGVFTVSYPRPTQVVIASLAASPREADGT
                     KPSVHALTTFPAQEIDGVFIKDAYLHLECVLDRIVDDFGENSLICGRIMTAHVEEDYL
                     RASDRDDNDLVHASPLLAYLSPGRYARIAESNSFPFPKDFSR"
     gene            1353..2522
                     /locus_tag="AC16CM_12580"
     CDS             1353..2522
                     /locus_tag="AC16CM_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3140714.1"
                     /translation="MQTSHREVLGYLHEHARSITAFLERLALAESPTASPESQGPVLA
                     ILAEAIAGVGYAVRHIPGREYGGHLYARPRAGRGPAQLLLGHCDTVWPVGTVKEMPVE
                     VGDGVVKGPGVYDMKGGLAQMVFALRALHEVGHRPTVTPIVFVNSDEERGSPESTRHI
                     RRLSRIAERTFVMEPSLGPSGKLKTARKGVGRFTVVVEGRPAHAGLDPEGGASAILEL
                     SYLIQALFSLNDPARGVTVNVGTIDGGLSPNVVAPKSSAVVDVRVPTQEDARRIAESI
                     LGLTPATPGVKLLVEGSIGRPPMERTSRNRRLWNAARRLGKEMGLELDEGLAGGGSDG
                     NTTSLFTATLDGLGAVGDGAHARHEFVLADKMVERAALLALLLMEPPLAENRSEP"
     gene            2519..>3214
                     /locus_tag="AC16CM_12585"
     CDS             2519..>3214
                     /locus_tag="AC16CM_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140715.1"
                     /translation="MKPRYIFASLTRISDLPDRQFSVEALPRRSWETGDYVVGEVVSG
                     AGGTPRIELSSGRMVEVARGDLIVGSFGVRYATLEAVGGWQGIGHDRGMEALTGAGLF
                     GRSTSRSVLMPPPLSLLYKGHVLVGKDKAAMRDYVARLPEREFGLPVVLLVGTSMSAG
                     KTTSAKVIVRLLREVGLSVIGAKLTGAGRYRDILAMQDAGAEHIFDFVDAGLPSTVVP
                     EKDYGKALRQLLRR"
BASE COUNT          576 a         1005 c         1097 g          536 t
ORIGIN      
        1 ggcgaaggtt atcgtgggcg acgacggctc gaagagcgcc gagcttgccg ccggacttgc
       61 cgcggacctg ggcggcctgt acgcggccga gatcactctg atccgggccc acacaaaccc
      121 gccggagccg atcggaggct ggagctctaa ggatcgcagc gggctcgacc tgatgaggtc
      181 gagagaggca gatgcgatcg cgggacgtgc cagggagata gagaaggtta ccggcacacg
      241 gccttcgatc cgtgtcctcg acaccgacgc cgtactggcc atgatgctcg ttgctgagaa
      301 gggtgaggaa cgcagaaccc tcatcgccgt aggtagccgt gggctcggtg tgattggccg
      361 agtgaggctc ggaagcgtat ccacaaagat tttgaggatc gccagaggtc ccgtcttgat
      421 ccacgcgggg tctctggacg agatcgatgc ccacgagcat ggcaaagccg cctccgtcgt
      481 gggacgacag gcacgtgtcc ctggcggatg acctggtaga gcggcaggat gttggcgatg
      541 ttggcaacgc tcatcccggg tttgttgggc atgtccacct tgcccgcccg cgccaccgct
      601 gatgatggcc tgaaacgtcc tggtcgatgt cggatctcca ctcccgggtg ccgcaggcga
      661 tcggatcgag gacgcggttg aaacgcgtgg tcgcctgccc ggccagtcca cgcatgcagg
      721 caaggaggcg ccatgacaga gcatgaccgc ggaaaactcg taacgctttc gggagatctc
      781 cccatctggg agcgggtctt cacggtcaac ccgctggtcg tggtcgggac cagggagagg
      841 gaaggagggt acgatctcgc cccgaagcac cgggcgtttc ccctgggctg ggacaactac
      901 ttcgggttcg tatgtacccc gcgccacggc acctacgata acgtcagacg cgagggcgtc
      961 ttcaccgtaa gctacccgag gccgacccag gtcgtgatcg cgagcctggc cgcctcgccg
     1021 agggaggccg acggcacgaa gccatccgta cacgccctga ccaccttccc cgcgcaagag
     1081 atcgacgggg tcttcatcaa ggatgcgtac ctccatctgg agtgcgtgct cgaccggatc
     1141 gtggacgatt tcggcgagaa cagcctgatc tgtggccgca tcatgacggc acacgtagag
     1201 gaggactacc tgcgcgcctc ggaccgggac gacaatgacc tggtccacgc ctcgccgctt
     1261 ctcgcctacc tgagcccagg ccgctacgcg cgcattgcag agagcaattc ttttccgttc
     1321 ccgaaggact tctcgaggta ggggctctta ccttgcagac gtcgcatagg gaggttctgg
     1381 gttacctgca cgagcacgca cggtccataa cggctttcct ggaacgcctc gcgctggcgg
     1441 agtcgcccac cgccagccct gaatcacaag ggcctgtgtt ggcaatcctc gcggaggcca
     1501 tcgccggggt gggctacgcc gtccgccaca tcccgggcag agagtacggg ggccacctgt
     1561 acgcgcggcc ccgggcgggc cgcgggcccg cacagctctt gctgggtcac tgcgacaccg
     1621 tctggccggt cgggaccgtg aaggagatgc ccgtcgaggt gggtgacggc gtcgtgaagg
     1681 gccctggcgt ctacgacatg aaaggcgggc tggcgcagat ggtcttcgcg ctaagggccc
     1741 ttcacgaggt gggccacagg cccaccgtca ccccgatagt cttcgtgaac tccgacgagg
     1801 agagaggaag cccggagtcc acccgtcaca tcaggcgtct ctcgcgtatc gccgagcgaa
     1861 cgtttgtcat ggagccatca ctaggaccgt cgggcaaact caagacggcc cggaagggag
     1921 taggacgctt caccgtcgtg gtcgaagggc ggccggcgca cgccggcctg gatccggagg
     1981 gcggggcgag cgccatcctg gagctctctt acctcatcca ggccctgttc agcctcaatg
     2041 atcccgcccg cggtgtcacg gtcaacgtgg gcaccataga cggtggcctg agccccaacg
     2101 tcgtggcccc gaagagcagc gccgtggtgg acgtgcgggt gcccacccaa gaagatgccc
     2161 gccgcatcgc ggagagtatc ctcggcctca cgcccgcaac gccgggcgtg aagttgctgg
     2221 tagagggctc aataggccgg ccgcctatgg agcgcacatc tcgaaaccgg aggctctgga
     2281 acgcggcacg caggcttgga aaggagatgg gtctggagct ggacgaggga ctggccggcg
     2341 gaggttctga cgggaacacc acgagcctct tcacggccac cctggacggg ctcggggcgg
     2401 tgggcgatgg cgcccacgcc cgccacgagt tcgtcctcgc ggacaagatg gtcgagcggg
     2461 ctgcgctcct ggcgctgctc ctgatggaac ccccactcgc cgagaaccgg agcgaaccat
     2521 gaaacctaga tacatatttg cctccctgac ccgtatctcc gacctgcccg acaggcagtt
     2581 ctccgtcgag gcactccccc gcaggagctg ggagaccggt gattacgtgg tgggagaggt
     2641 cgtttctgga gcgggtggaa ccccacggat cgagctgtcg agcggccgga tggtcgaggt
     2701 ggctcggggg gacctgatcg tcgggtcctt cggcgtgcgg tacgcgacgc tggaggcggt
     2761 tggtggctgg caagggatag gtcacgatcg tgggatggag gcacttaccg gcgcgggcct
     2821 cttcggaagg tccacctcca gatcggtcct catgccccct cccctctccc tactctacaa
     2881 ggggcatgtg ctggtcggta aagacaaggc ggcgatgcgc gactacgtgg cccgcctacc
     2941 cgagcgtgag ttcgggctcc ctgttgtgct gctggtcggg acctcgatgt ccgcgggaaa
     3001 gaccacctcg gcaaaggtca tcgtgcgcct cctgagggag gtgggacttt cggtaatcgg
     3061 ggcgaagctg acaggggcgg gacgttacag ggacatactc gccatgcagg acgccggcgc
     3121 ggagcacatc ttcgacttcg tcgacgctgg cctgccttcg accgtggtcc cggaaaagga
     3181 ttacgggaaa gccttgcgcc agctcctccg gcgc
//
LOCUS       DBNGVD010000468         4253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3209835, whole genome shotgun sequence.
ACCESSION   DBNGVD010000468 DBNGVD010000000
VERSION     DBNGVD010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3209835"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16CM_12590"
     CDS             <1..479
                     /locus_tag="AC16CM_12590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140716.1"
                     /translation="NMLTETTEFEDSDFEAYLQELVWPLLRFVRAALPQMIERRSGKI
                     VGVTSATPLRAIPGLSVYSAARGAQNAFLQVVGTEAAPHNVQVNAMGPAHIANNMYYT
                     SEMLEDDEVREQFESQIPAGRIGDGQEAAELVLALATGASGFLAGQVIPISGGWVT"
     gene            534..1511
                     /locus_tag="AC16CM_12595"
     CDS             534..1511
                     /locus_tag="AC16CM_12595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3140717.1"
                     /translation="MEIFENLSASSRLESAELDAYSQAVRDVTEKLEPAVISVGLPDG
                     RGGGSGVILGVAGDSATAITNSHVVRGLRGRRSGNPGGDIQVALSDGSSSPAEVLGDD
                     PPSDLAVVRFDPEEEPTVATLGDAEDLAVGQLVVAIGNPLGFQRSVTTGVVSALGRSI
                     RGESGRLIENVIQTDAAINPGNSGGPLADAAARVVGINTAIIGGAQGIGFAVPVSGTF
                     RRIVFTLVMEGRVRRAFLGVVVESHQAQGEFAGGAGVRNVAPNSPAERAGVRPGDVIV
                     GMKGEPVHSNDDLLNLLDVSSIGQDTTLRVLRGNKEIPLTVRPREQPEE"
     gene            1711..1947
                     /locus_tag="AC16CM_12600"
     CDS             1711..1947
                     /locus_tag="AC16CM_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR3140718.1"
                     /translation="MPDVTFLVNDLAGAEEAEKLQRALTRLNFVDLVNVDSEKGLVAV
                     SYAGGDEELGRIGDAIEGAGHAAEPSPGADAGLD"
     gene            1951..2379
                     /locus_tag="AC16CM_12605"
     CDS             1951..2379
                     /locus_tag="AC16CM_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140719.1"
                     /translation="MPEPSIPVAEAARFLDVPEGEAQALAGADGKVSLETVRSRAGAA
                     AIAGELARAREEAGRLAGQLKEVRATNLRLQRELKDSVEERRRLTGEVLELRAAAEER
                     LMLMERIEHIAHVERELEESSSELERLRNRTLLARLLNRG"
     gene            complement(2582..3310)
                     /locus_tag="AC16CM_12610"
     CDS             complement(2582..3310)
                     /locus_tag="AC16CM_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3140720.1"
                     /translation="MELFSGAVLAHLVEVFWVNIILSGDNAVVIAMAVRTLEGTNRRR
                     AVLWGAAGAVVLRLIFAAVIAFLLHVPLLQVVGGVLLIWIAWQLVRDGGGEGDDIKAG
                     QSTWEAIRIIIVADVVMSLDNVVALVQAAEVGSEVNYAILGIGLITTVPLVVFGATLL
                     TSLLNRFPVLVYFGASLLVYLAIEMFFEDVVTHPYLEPFMRIEWIVGIGAAALFVLIA
                     WLAGRKAGGLAEDRKGAGDGPNAD"
     gene            complement(3396..>4253)
                     /locus_tag="AC16CM_12615"
     CDS             complement(3396..>4253)
                     /locus_tag="AC16CM_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR3140721.1"
                     /translation="IVAILGAVIVGTGAAAAGEIGVGAILGAPFLLATLALFLVGLSA
                     IVYKDRRKSGDEVIADREVTIPDLGFFLVCYTLATGAGVVRLPLVLKIILAILLLLAY
                     AYYVIRTIRKGGSATEGEAPENLTLWPFGSTAPTWAVATQLVGTVVVMGFGAHFFVDA
                     VGHMSLAVGIPAGLISLVLAPLATELPEKFNSIIWMRNNKDTLALGNITGAMVFQSTI
                     PVSIGILFTPWELGFLSAFSAVLALASGVVFIGFLLRQRPLRGFVMLGAGSLYVVFLV
                     AAVFAVIAA"
BASE COUNT          832 a         1334 c         1417 g          670 t
ORIGIN      
        1 ccaacatgtt gacggagacc accgagttcg aggactcgga tttcgaggcc taccttcagg
       61 agctggtctg gccgctcttg cgtttcgtgc gtgccgcgct gccccagatg atcgagcgcc
      121 gctccggaaa gatcgttggc gtcacgagcg cgacgccgct gcgtgctatc ccgggcctga
      181 gcgtgtatag cgccgccagg ggcgcccaga acgcgttcct gcaggtggtc ggcacagagg
      241 ctgcgcccca caacgtacag gtcaacgcca tgggcccggc gcacatcgcg aacaacatgt
      301 actacacctc cgagatgctc gaagacgatg aggtcaggga gcagttcgag tcccagattc
      361 ccgccgggcg aatcggtgat ggccaggagg ccgccgagct cgtgctggcc ctcgccaccg
      421 gcgcaagcgg tttcctggcg gggcaggtga taccgatctc cggcgggtgg gttacctgat
      481 acctggccgt tcttccgggt ggcgcgcccg gggcggtgcg gtacaaactg aacatggaga
      541 tcttcgagaa tttgtcggcg agttcgaggc tcgaaagcgc ggagctcgat gcctactcgc
      601 aggcggtacg tgacgtaaca gagaagctcg agccggccgt gatctcggtc ggattgcccg
      661 acggtcgtgg cgggggaagc ggcgtgatcc tcggggtcgc gggtgactcg gccaccgcca
      721 tcacgaacag ccacgtggtc cgcggcctgc gcggacgcag gtccggaaac ccgggtgggg
      781 acatacaggt agcgctctct gacgggtcgt cgtcgccggc cgaggtgctg ggcgacgacc
      841 cgccgagcga tctcgcggta gtccgcttcg accccgaaga ggagccgacg gtggccacgc
      901 taggcgacgc cgaggacctc gcggtcggcc agctcgtcgt ggccatcggg aaccccctcg
      961 gtttccagcg gagcgtcacg accggggtgg tgagcgccct gggacggtcc atcaggggcg
     1021 agagcggcag gcttatagag aacgtgatcc agacggacgc cgccatcaac ccgggcaact
     1081 cgggggggcc tctggccgac gccgcggccc gcgttgtcgg catcaacacg gcgatcatcg
     1141 gcggcgcgca gggcataggc ttcgccgtac ccgtctcggg aacgtttcgc cgcatcgtat
     1201 tcaccctcgt gatggagggt cgggtgcgcc gcgcctttct tggggtcgtg gtggagagcc
     1261 accaggccca gggcgagttc gcgggcggtg ccggcgtgag aaacgtcgcc ccgaacagcc
     1321 ctgccgaacg cgcgggcgtg agacccggcg acgtgatcgt cggcatgaag ggagagcccg
     1381 tccactccaa cgacgacctc ctgaacctgc tagacgtctc ctccataggc caggacacca
     1441 cgctacgcgt cctgcggggc aacaaggaaa tcccgctcac agtgaggccc cgagaacagc
     1501 cggaggaata gctgtttcag ctgtcagctc ttagttatca gctttcagcc aggaatggtc
     1561 tggcgaacct tgtggatgac aacggaccgt cgatagacca aacttaccct cctggaatgg
     1621 cagcgtgatc gtgcgacgac cacgctacgt cggtagagtg ctaacagctg aaggctgaca
     1681 gctgaaaacc gagcaaccga agggagcgat gtgcctgacg taacttttct ggtgaacgac
     1741 ctcgcggggg cggaggaagc cgagaagctg cagcgggctt tgacgcgcct gaatttcgtg
     1801 gacctggtga acgtcgactc ggagaagggg ctcgtggcgg tttcttacgc cgggggagac
     1861 gaggagctcg gtcgtatcgg ggacgccatc gagggtgctg ggcatgcggc agagccctcg
     1921 ccgggcgcgg acgcaggcct ggactgagcg gtgccggaac cctccatccc ggtagccgag
     1981 gcggcccgtt ttctggatgt acccgaaggg gaagcgcaag ccctggccgg cgcggacggg
     2041 aaggtgtccc tggagacggt caggagcagg gccggggcgg ccgccatcgc cggcgagctg
     2101 gcccgggcgc gcgaggaggc gggacggctc gcgggccagc taaaggaggt ccgcgccacc
     2161 aacttgaggc ttcagcgaga gttgaaggac tccgtagagg agcgtagacg cctcacgggg
     2221 gaggtactgg agctcagggc cgccgccgaa gagaggctga tgctgatgga acgcatcgag
     2281 cacatagccc acgtcgaacg ggaactggag gagagctcct cagagctcga acgcctcagg
     2341 aacaggacgc tactggcgag gctcctgaac cgcggctgaa gcccacggtg ggcgtcgctt
     2401 gcagagatgt cgaacccgtg tttcacgtgc tgcatggtcc cctacgaaac gccctacagt
     2461 ggtttgcgga ggaacgtccc ttctcgaaga tagccgtcgg ctttcgcggt cgggttttcc
     2521 cggctgatgg ctgaccgctg atagctcgct ctccacaggg ccagtcgttc ggcaaaccgc
     2581 tctaatctgc gttgggaccg tctccggcgc cctttcggtc ctcggcgagc cctcccgcct
     2641 tccgccccgc cagccacgcg atcaacacga acaacgcggc ggccccgatc ccgacgatcc
     2701 actcgatcct catgaagggc tccaggtagg gatgggtgac cacgtcctcg aagaacatct
     2761 cgatagccag gtaaacaagc agactggccc cgaagtagac cagcaccggg aaccggttca
     2821 agagcgacgt tagcagggtg gccccgaaca cgaccagagg caccgtcgtg atcagtccta
     2881 taccgagtat tgcgtagttg acctctgagc cgacctctgc cgcctggacg agggcgacca
     2941 cgttatccaa agacataacg acgtcggcca cgatgatgat ccttatcgcc tcccacgtgc
     3001 tctgcccagc cttgatgtcg tcgccctcgc cgccgccgtc gcggaccagc tgccaggcga
     3061 tccagatgag caagacgccc ccgaccacct gcaacagggg cacgtgcagc aagaaggcga
     3121 tcaccgcggc gaagatcaga cgcaacacca cggcccccgc cgcgccccac aacacggccc
     3181 tgcgacggtt ggtaccctcc agggtgcgta cggccatggc gatgaccacc gcgttgtcgc
     3241 cggagaggat gatgttcacc cagaacacct ccacgaggtg ggccagcacc gcgccactga
     3301 atagttccat ctaggctcgc gtctccatga tcgctatctt cacaaaggat atgagtttac
     3361 cagccaatcg tgccggctac cgcggctggg agatatcaag cggcgatgac ggcgaaaacc
     3421 gcggcgacca ggaagaccac gtacagcgaa cccgcgccga gcatgacgaa tccgcgcagc
     3481 ggcctctggc gcagcaggaa gccaataaag accacgccag aggcgagggc taggacggcg
     3541 gagaacgcgc tcaggaagcc aagttcccac ggtgtaaaca gtatgcctat agaaacgggg
     3601 atggtactct ggaacaccat cgcgcccgtt atgttgccca gggcgagggt gtctttgttg
     3661 ttcctcatcc agatgatcga gttgaacttt tcgggaagct ctgttgccag cggcgccagg
     3721 accagcgaaa tgaggcctgc cgggatgccg acggccaacg acatgtgccc tacggcatcg
     3781 acgaagaagt gggcgccgaa gcccatcacg acgaccgtgc cgacgagttg ggtcgccacg
     3841 gcccacgtcg gcgcggttga gccgaagggc cagagggtga ggttctcggg ggcctctccc
     3901 tcggtcgcgc ttccgccctt ccttatggtt cggatgacgt aatacgcgta ggcgaggagg
     3961 agcagtatgg ccagaataat cttcaggacc aggggcaagc gcacgacgcc ggccccggtg
     4021 gccagcgtgt agcagacgag aaagaaaccg aggtccggta tggtcacttc gcggtccgcg
     4081 atgacctcgt cccccgactt tctgcgatct ttgtagacga tggccgagag ccccaccagg
     4141 aacaaagcca gtgtggccag caagaacggg gcgcccagaa tcgcaccgac gcctatctcg
     4201 ccggctgcgg ccgctcccgt gcccacgatg accgcgccga gtatggccac gat
//
LOCUS       DBNGVD010000469         3796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1206465, whole genome shotgun sequence.
ACCESSION   DBNGVD010000469 DBNGVD010000000
VERSION     DBNGVD010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1206465"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1587
                     /locus_tag="AC16CM_12620"
     CDS             166..1587
                     /locus_tag="AC16CM_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140722.1"
                     /translation="MAASTVGTIIEWYDFALYGAASALVINELFFPTAGPVIGTLAAF
                     ATFAVGFFARPLGGVVIGHYGDRLGRKPALIFTVVLMGLSTVLIGLLPTYEQIGVWAP
                     ALLVLLRLLQGFGAGAELAGAITYIAEYTPASKRAFYTAIPNGATAAGLLLATGAFSI
                     ISFLPEEQLLSWGWRLPFVASVVILLVAIFIRQRLQESPAFREELSEGEDPPRLPVRE
                     VLADRPRDVLCGFLSVSGHNANAYVLNTFALSYITATLGLAQGVGLLALFVAAGTGII
                     CAPLFGMLADRIGRRPVFIGGATFIALFAFPFFYLLDTKSPALVVLAMTLGYGIGFGA
                     TSGAQGAFLSELFETRYRYTGISLARELNGVAIAGPTPFIAAALVALAGGSPWFVAIY
                     VIFCQSSRSSASFTPEGSTHPSRIPSKNSATRAWSAASGEQANEDPRRPTSSVVVRRT
                     ESTPRASRKAGDQFEVKLCLAGP"
     gene            2397..2765
                     /locus_tag="AC16CM_12625"
     CDS             2397..2765
                     /locus_tag="AC16CM_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140723.1"
                     /translation="MTHRELEEEIVAPTDPSPNQADEMRVRYGYRVIIAGFILVALVA
                     AVSIFKWDSAQSASLVLGLVTSMVGTVVGAFFGVQVGAQGKAEAEAARDEAQKKAEAA
                     LQTLRPDQSDQFRKIYQEQR"
     gene            complement(2798..3331)
                     /locus_tag="AC16CM_12630"
     CDS             complement(2798..3331)
                     /locus_tag="AC16CM_12630"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140724.1"
                     /translation="MGKLIATTQATIDGVIDPVGEWVRADGGHGEYSFERQARSGGLL
                     LGRKTYEGLAGYWPGQTGKWADMVNALPKYVGSNTLSGDLAWNATLLEGNLEYSIPKL
                     KDEVDGDLFMHGSGEFAYALAERGLIDEYEVYLNPLVWGRGNVHVLGDRGTVWMRLAD
                     LKRFDSGVVLITYLPAS"
     gene            complement(3416..>3796)
                     /locus_tag="AC16CM_12635"
     CDS             complement(3416..>3796)
                     /locus_tag="AC16CM_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140725.1"
                     /translation="GTFEPAGGWGGDHHDGVQIWVLSRREPGIDVSRWPLVTYVSDVR
                     TAMTEARRAAGDKNVLVHGATTAQLALAAGVLDEIEIHLIPVLLGGGRRLFDNLGSEH
                     IELERTRVLEGEDGVTHMHFRVQR"
BASE COUNT          654 a         1223 c         1202 g          717 t
ORIGIN      
        1 gggcaataga ggaagggatg tctactgagg aggccttcgg ccgctacggt gtcatgtgag
       61 cccgcacgac gatgacttta acggggaggg atcctgagcg agggtacgag caacgctgga
      121 ggcggccagc ggggggagat aagctcggtc gacacccgac ccgtcgtggc ggcgagcacg
      181 gtcgggacca tcatcgaatg gtacgacttc gcgctgtacg gcgcggcctc ggccctcgtc
      241 ataaacgaac tcttcttccc cacggcgggt ccggtcatcg ggacgctcgc cgcgttcgcg
      301 accttcgccg tggggttctt cgctcggcct ctaggcgggg tcgtgatcgg ccactacggg
      361 gacaggctag ggcgcaaacc cgccctcatc ttcaccgtgg ttctgatggg gctttccacc
      421 gtcctgatcg ggcttctgcc cacctacgag cagatcggcg tctgggctcc ggcgctgctc
      481 gtgttgctgc gcctgctcca gggcttcgga gccggggccg agctagccgg ggcgataacc
      541 tacatcgccg agtacacccc cgcgagcaag agggccttct acaccgcgat cccgaacggc
      601 gcgacggccg cggggctgtt gctggcgacg ggcgctttct ccattatctc gttcttgcca
      661 gaggagcaac tgctctcctg gggctggcgc ctgccgttcg tcgcgagcgt ggtgatcctg
      721 ctcgtcgcca tctttatacg gcagcgcctc caggagtcac cggccttccg cgaggagctc
      781 tccgaaggcg aggatccgcc acgacttccg gtcagggaag tcctcgccga ccgcccccga
      841 gacgtgctct gtggttttct ctcggttagc gggcacaacg cgaacgccta cgtcttaaac
      901 actttcgccc tcagctacat cacggccacc ctcgggctgg cgcaaggggt cgggctgctc
      961 gcgctcttcg tggccgccgg aacgggaata atctgcgccc cgctcttcgg tatgctcgcc
     1021 gaccgaatcg gcagacgtcc ggttttcatc ggcggcgcca cctttatcgc gctcttcgcg
     1081 ttcccgttct tctacctcct cgacacgaag tcaccggcgc tagtcgtttt ggcgatgacg
     1141 ctcgggtacg ggatcggctt cggcgcgacc tctggggccc agggcgcctt cctttcggaa
     1201 ctcttcgaga cccgctacag gtacacgggc atctccctgg cccgggagct caacggggtc
     1261 gcgatagccg gtcccacgcc gttcatcgcg gccgcgctcg tggcgcttgc cggaggatct
     1321 ccctggttcg tcgcgatcta cgttatcttc tgccagtcct cacggtcgtc ggcgtcgttt
     1381 acgccagagg gctcgaccca tccgagcagg atcccctcga agaactcggc gacccgggca
     1441 tggtcggccg ctagcgggga gcaggcaaat gaagacccgc gccggccgac gtcctcggtt
     1501 gtagtgaggc ggaccgaaag cacgccgagg gcgagcagaa aagctggaga tcagtttgaa
     1561 gtaaagttat gtctggcagg tccgtgaggc agaactacgc cgccacgaca agcggatctg
     1621 caatcagcaa tctcattccg gcagcgtagt tgacgcgcgc cagtacagta ggaaaatatc
     1681 cgcctggcag atgcgccggt cctcacaagc tgtcattctt ctgcttgcaa ggcggtccgg
     1741 cggggaccgc tacgacgtca ggaggatgta tgaggcgaac gcttacggtg ctttcggtaa
     1801 tggcgctcat ggtggctgcg ttggcggtgc cagcgtttgg ggcggccaac ccgaaggcca
     1861 gctgcgtggg tacggtacac agtaaccaga ctgagccggg ggcagccgga gatcttcata
     1921 gctcgcttgg tcgggaggga acgaacgggg attttgccag gttcttctct catgtgggaa
     1981 cgccgggcca gagagctact actggcgacc ttacccctga cagcgcattc tgtgtagatc
     2041 taggatcatc ctaggctgat agagagtacc tggtccgagc gcggaccttc gtagggccgg
     2101 ggccacgaac cccggctcct tccgtcaacg ccgaatatgt ccacattccc caaaaggaga
     2161 gcgagatcct gttggtcggt ccgccccgcg ccgtcgaata atcctttgcg atgctggttc
     2221 ggaacaccat attccacctg aaccctggaa gatagccgca gaaagagcgg ccgggcgtga
     2281 tgagcaagag cgtgaggccg ttgtctgcgt ctccgaagac ctggcaagac ctcgtgcccg
     2341 aatatacgcg atctggggga tgcgtcggtt acgcacgatt gttagggtgg cgtcctgtga
     2401 cccatcgtga gctcgaggag gagatcgtgg ccccgaccga cccgtcccca aaccaggcgg
     2461 atgaaatgcg cgtacgctac ggctaccgag tgatcatcgc ggggtttatc ctggttgccc
     2521 ttgtcgcagc cgtgtcgatc ttcaagtggg attcggccca gagtgcgtcg ctcgtgctcg
     2581 gcctggtcac cagcatggtc gggaccgtgg tcggtgcgtt cttcggcgtt caggtagggg
     2641 cgcaagggaa ggctgaggcg gaggccgcac gcgacgaggc ccagaagaag gcggaagcgg
     2701 ccctgcagac gttacggccc gaccaatcgg atcaattccg gaagatctac caggagcaac
     2761 gctagatgag caaaaaccaa ctacggggtc cttgccttca agacgccggt aggtaggtga
     2821 tgaggacgac cccggagtcg aaccgcttca ggtcagcgag cctcatccag acggtccccc
     2881 tgtcgccgag cacgtggacg ttcccccggc cccagacgag cgggttcagg tacacctcgt
     2941 actcgtcgat cagacccctc tcggcgaggg cgtacgcgaa ctcgccgctg ccgtgcatga
     3001 acaggtcacc gtcgacctcg tccttcagct tcggaatcga gtattcgagg ttcccctcga
     3061 gcagggtcgc gttccacgcg agatccccgg acagggtgtt ggacccgacg tacttcggaa
     3121 gcgcgttgac catgtccgcc cacttgcccg tctggcccgg ccagtacccg gccagcccct
     3181 cgtaggtctt gcggcccagc agcagaccac cggatctcgc ctgacgctcg aacgaatact
     3241 caccgtgtcc cccatccgcc cgcacccact cgccaaccgg gtctatgacc ccgtctatgg
     3301 tggcctgcgt ggtggcgatc agcttcccca tccctattct ccttccatgg gtttgccctg
     3361 ccggcagtgt tgcgcgacca aacagagaga gatccaccag gaggtagaca tccgcttacc
     3421 tctggactcg gaagtgcatg tgcgtgaccc cgtcctcacc ttcgaggacc cgggtacgct
     3481 ccagctcgat gtgctcggat ccgaggttgt cgaacaggcg gcgtcctccg ccgagaagta
     3541 ccgggatgag gtggatctcg atctcatcga ggacgccggc cgcgagcgcg agctgcgcgg
     3601 ttgtggcgcc gtggaccagc acgttcttgt ctcccgcggc ccgcctggcc tcggtcatcg
     3661 ccgttctgac gtcgctcacg taggtgacca gcggccaccg gctcacgtcg atgcctggct
     3721 cacggcggct gaggacccag atctgcactc catcatggtg gtcgccgccc cagccgcccg
     3781 ccggctcgaa ggtccc
//
LOCUS       DBNGVD010000470         6717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_113320, whole genome shotgun sequence.
ACCESSION   DBNGVD010000470 DBNGVD010000000
VERSION     DBNGVD010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_113320"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16CM_12640"
     CDS             <1..706
                     /locus_tag="AC16CM_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3140726.1"
                     /translation="KLVGIHSPGGFAEFTLAPASNVYPLPEGVEARTGALAEPLANGV
                     HAARLGLAGRSVEHAVVIGAGTIGLMCLQAAVLDGIPEVHAVEPVEGRRGQALALGAD
                     AAHASGEEAREALDGPTGGLGVDLVIDAVGAEATRRMALELLRPGGRAVYIGLHDDDT
                     TLGFHDVVRGQLDVQGSYAFTAEDYEQALEWLVAGKAGIGELPPVLPLEEGPGAFAEL
                     VRGPSDRIKIFLAGAE"
     gene            706..1470
                     /locus_tag="AC16CM_12645"
     CDS             706..1470
                     /locus_tag="AC16CM_12645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3140727.1"
                     /translation="MEGASLSGKTALVIGASSGIGLATANLFADAGATVHAAARRRQT
                     IEEGVGGRNVAAHSLDISDGDAVWRLVEEVGEDGRIDVLVVAAGMNFPERRLEQLTAD
                     GWDSMVAVNLSGAFYAIRAALPYLRASRGLVVLISSVSGRWPDASGPAYQATKAGMVE
                     LGHAAGFEEHVNGVRFSSILPGIVDTPILDNRPEPPPKEVRDAALKPEDVAQACLFLA
                     TLPPHAYVPELTMVPTSIQALGKTSTATPPVSRGDE"
     gene            1498..3276
                     /locus_tag="AC16CM_12650"
     CDS             1498..3276
                     /locus_tag="AC16CM_12650"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR3140728.1"
                     /translation="MISDNGHPARRFRSRDWFDDPHRRDQSALYLERFMNYGITPDEL
                     RTGRPIVGIAQSGSDLSPCNRHHVELAGRVRDGIRDGGGIPMEFPVHPINENCRRPTA
                     ALDRNLSYLGLVEILYGYFIDAVVLTTGCDKTTPAAIMAASTVDIPAIVLSGGPMLDG
                     YFEGEMAGSGTVVWKSRRRLAAGEIDEEEFYEAALASAPSAGHCNTMGTASTMNALAE
                     VLGMALPGCAAIPAPYRERGQMAYETGKRAAEMAYEDLRPSDILTRESFLNAIVATTA
                     LGGSTNAQPHIMAMARHAGVELDPADWERGHDVSLIVNMQPAGEYLGERFHRAGGVPA
                     VVNELLRAGKIDGETTTVTGRTLAESVVGRESVDRGMITSYERPLQEKAGFRVLSGNL
                     FDFAIIKTSVISDDFRRRYLSDDDVFEGRAVVFDGSDDYHKRINDPALNIDERSILVI
                     RGAGPIGWPGSAEVVNMQPPDALIKRGIDTLPTIGDGRQSGTSDSPSILNAAPESAVG
                     GGLAWLRTGDTIRIDLDAGRCDMIVPEEEIERRKREDGIPEFPESQTPWQEIYRSSVN
                     GLDYGAVLEFAPKYRGVASRTPRHNH"
     gene            3422..3772
                     /locus_tag="AC16CM_12655"
     CDS             3422..3772
                     /locus_tag="AC16CM_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140729.1"
                     /translation="MERMMIVEDHSAFAQALGLVLGANTQISHARTLREGRTLLRNGE
                     PFDLVVLDLSLPDGVGTELVPEIKDRDPRTSVVVLSAREDIAEVAAEAGADAAIPKET
                     PLPDIIATLRSLAG"
     gene            complement(3793..4143)
                     /locus_tag="AC16CM_12660"
     CDS             complement(3793..4143)
                     /locus_tag="AC16CM_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140730.1"
                     /translation="MLALGFMIGLVVSFVILTGGPEPATTLLLMIAMFTLFGYLMRLL
                     TGRRPRGPKTGGEKQLLMSIVDAGGSITPVEAALRTSLTVDEAEETLTRFANRGHLVV
                     HSRDGALLYAMPGR"
     gene            4321..5076
                     /locus_tag="AC16CM_12665"
     CDS             4321..5076
                     /locus_tag="AC16CM_12665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HIR3140731.1"
                     /translation="MTLSEEVAWVTGSSTGIGRAVAVELAAGGRRVVVHYNASEDEAR
                     EVVKEIEGYGGEASLVGGDVSDADEVGRMVGEIENRFGRLDVLVNNAGSLIERRGISA
                     MTEDLWDRVMDVNLKSVYLVSQAALPLMREGGRGRIINITSVAARNGGGPGSVAYATA
                     KGGVSTLTRAMAKELVSENILVNGVAPGVITTPFHDRFTPPDVRENLKSAIPMGREGT
                     PEETAGVVAFLASPAADYLVGEIIEVNGGQLMS"
     gene            complement(5122..5757)
                     /locus_tag="AC16CM_12670"
     CDS             complement(5122..5757)
                     /locus_tag="AC16CM_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3140732.1"
                     /translation="MRTILCYGDSNTWGFDPETRERFPEDVRWPGVFRARLGDDYRVI
                     EEGLSGRTTVWDDPIEGVHKNGRTYLRPCLESHSPVDLVTIMLGTNDLKSRFGSSASD
                     IAQGAAGLAEMVTRSGCGPGGGAPVVLLISPPPVATLTDMAQMFEGAEGKSRQFPEHY
                     ERFAEQHGCEFFDASTVVMSSDVDGIHLDAGEHRKLGEAVSARVEQLFGRR"
     gene            5922..6083
                     /locus_tag="AC16CM_12675"
     CDS             5922..6083
                     /locus_tag="AC16CM_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140733.1"
                     /translation="MSIVKAPGTLDVASVERLCFPNVTRDRVTAANPAGPRPESVLRN
                     DPSRRARTG"
     gene            6210..>6717
                     /locus_tag="AC16CM_12680"
     CDS             6210..>6717
                     /locus_tag="AC16CM_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR3140734.1"
                     /translation="MEQNSEGPRIDSTVFWVAAVLSAVFVAWGIFFTESLAAVFDAVL
                     WSFLVPNFGWMFILSTFGFLSFALYLAFSRFGKIRLGAQDEQPEFSTVSWVAMMFSAG
                     MGIGLMFDGVAEPLSHMGAPPFGLAEANTKAGAQVAMEYSFFHWAFHPWAIYAIMGLA
                     LAYFTFRKG"
BASE COUNT         1141 a         2184 c         2274 g         1118 t
ORIGIN      
        1 gaagctcgtt ggcatccact cccccggcgg cttcgccgag tttacgctcg cgccggcctc
       61 caacgtctac ccgctccctg aaggcgtcga ggcccgcacg ggcgcgctcg ccgagccgct
      121 agccaacggc gtccacgccg cgcggctcgg gctcgccggc cgttccgtgg agcatgccgt
      181 cgtgatcggg gctggcacca tcggcctcat gtgcctgcag gccgcggttc tcgatggcat
      241 ccccgaagta cacgccgtgg aacctgtgga aggccgccgc gggcaggccc tggccctcgg
      301 ggccgacgcc gcccacgcct ctggcgagga agcccgcgag gcactcgatg gtcctacagg
      361 agggctcggg gtcgacctgg tcatagacgc ggtgggggcc gaggcgacgc gccggatggc
      421 cctcgagctc ttgcggccgg gcggacgcgc cgtctatata ggtctgcacg acgatgacac
      481 gacgctcggg ttccacgacg tggtgcgggg ccagctcgac gtgcagggct cgtacgcctt
      541 cacggcggag gactacgagc aggcgctcga gtggctggtc gccggtaagg ccggcatcgg
      601 ggagttgccg cccgtgttgc cgctcgagga ggggccaggg gcgttcgccg agctggttcg
      661 gggtccttcg gaccggatca agatctttct cgcgggggcc gagtagtgga gggcgcgagc
      721 ctctcgggca agacggcgct cgttattggg gccagcagcg gcatagggct agccaccgcc
      781 aacctgttcg ccgacgcggg cgccaccgtc cacgccgcgg cccgccgcag gcagacgata
      841 gaagagggcg tgggaggccg caacgtcgcg gcccactctc tagacatctc ggacggggac
      901 gccgtctggc ggcttgtcga ggaggtcggg gaggacggca ggatagacgt gctcgtcgtg
      961 gcggccggga tgaactttcc ggagcggcgg cttgagcagt tgaccgccga cgggtgggac
     1021 tccatggtcg ccgtgaacct tagcggggcc ttctacgcca taagggccgc gctgccttac
     1081 ctcagggcct cgcgggggct ggtcgtcctg atctcaagcg tctccggacg ctggccagac
     1141 gcctccggtc ccgcctatca ggccaccaag gccggcatgg tcgagctcgg gcacgcagct
     1201 ggcttcgagg agcacgtaaa cggtgtgcgc ttctcctcca tcctgcccgg tatcgtcgac
     1261 acccccatcc tggacaaccg tccagagccg ccgccgaagg aggtccggga cgccgccctc
     1321 aagccagagg acgtggcgca ggcgtgtctc ttcctcgcca cgcttccgcc gcacgcctac
     1381 gtcccggagc tgaccatggt ccccacctcg atacaggcgc tgggcaagac ctcgacggcg
     1441 acgcccccgg tctcccgggg agatgagtag catccagatg aaacgaggag agatcccatg
     1501 atttcggata acgggcatcc cgcccgcagg tttcgttcgc gtgactggtt cgacgatccc
     1561 caccgcaggg atcagagcgc gctgtacctg gagcgcttca tgaactacgg gatcacgccc
     1621 gacgagctgc gtacgggcag gcccatcgtg ggcatcgccc agagcggcag cgacctctcg
     1681 ccctgcaaca ggcatcacgt cgagctggcc ggtcgggtgc gcgacggcat acgcgacggc
     1741 ggcggtatcc ccatggagtt cccggtgcat cccatcaacg agaactgccg caggccgacc
     1801 gccgcgctcg accgaaacct ctcttacctc gggctggtgg agatcctcta cggctacttt
     1861 atagacgccg tggttctcac gacgggctgc gacaagacaa cgccggcggc tattatggcc
     1921 gcctcgaccg tggatatacc cgcgatcgtg ctctccgggg gccctatgct cgacggctac
     1981 ttcgagggcg agatggccgg ctcgggaacg gtggtctgga agagccgccg gcgcctcgcg
     2041 gccggcgaga tcgacgagga ggagttctac gaggcggccc tcgcctcggc gccgtccgcg
     2101 ggccactgca acaccatggg aacggcctcg accatgaacg ccctcgccga ggtgctcggc
     2161 atggccctgc cggggtgcgc cgcgatccca gccccctacc gcgagcgcgg ccagatggcc
     2221 tacgagacgg gcaaacgagc cgccgagatg gcctacgagg acctgcgccc ctcggatata
     2281 ctgacgcgcg agtccttcct gaacgcgatc gtcgcgacca cggccctcgg cggctcgacc
     2341 aacgcccagc cgcacatcat ggcgatggcg cggcacgccg gcgtcgagct tgacccggcc
     2401 gactgggagc gcggccacga cgtgtccctg atcgtcaaca tgcagccagc cggcgagtac
     2461 ctgggcgagc gattccacag ggccggcggc gtgccagccg tggtgaacga actcctgcga
     2521 gccggcaaga tcgacgggga aaccacgacc gttacggggc ggaccctggc cgagagcgta
     2581 gtcgggcgcg agagcgtgga ccgcgggatg atcacctcct acgaacgacc gctgcaagag
     2641 aaggcgggct tcagggtgct ctccggcaac ctcttcgact tcgccattat caagacgagc
     2701 gtgatctcag acgactttcg ccggcgttac ctgagcgatg acgacgtctt cgagggccgg
     2761 gccgtggtct tcgacggctc cgacgattac cacaagcgca tcaacgaccc cgcgctgaac
     2821 atcgacgaac gctccatcct cgtgataagg ggggcagggc cgatcgggtg gccgggttcg
     2881 gccgaggtgg taaacatgca gccccccgat gcgcttatca agcgggggat agacacgctg
     2941 cccaccatcg gagacggcag gcagtcgggg acctcggaca gcccctcgat cctgaacgcc
     3001 gcgcccgaaa gcgccgtggg tgggggcctg gcctggctcc ggacgggtga caccatccgt
     3061 atagacctgg acgccgggcg ctgcgacatg attgttcccg aggaggagat agaacgccgc
     3121 aagcgggaag acggcatacc cgaattcccc gagagccaga cgccgtggca agagatctac
     3181 cgctcctcgg tgaacggtct cgactacggt gccgtcctcg agttcgcccc gaagtaccgt
     3241 ggcgtggcct cccgaacgcc caggcacaac cactgatccc gaatcggggt ggatggtgcg
     3301 gtggcaggct gccggattcg gtatgatggg tgttccatgc agtctctgat agggtattgc
     3361 tccgtgtctc tgtcacagtt ccctgttccc cggatcccgc ggatggcgcg gaggtagggc
     3421 gatggagcgt atgatgatcg tggaagacca ctcggcgttc gcacaagccc tcgggctggt
     3481 tctgggagcg aacacgcaga tctcgcacgc ccgcacgctg cgggagggcc gtaccctgct
     3541 acggaacggt gagcccttcg acctggtggt gctcgacctc tcgctcccgg acggcgtggg
     3601 aacggagctg gtgcccgaga tcaaggatcg agacccccgg acgagcgtcg tggtcctgag
     3661 cgccagggag gacattgccg aggtcgccgc ggaggccggc gccgacgcgg cgatcccgaa
     3721 agagacgccc ctgcccgata tcatcgccac cctgaggagc ctcgcagggt aaggtttcgg
     3781 ggccggggaa gatcacctcc ccggcatggc gtagagcagg gcgccgtcgc ggctgtggac
     3841 cacgagatga ccgcggttcg cgaaccgggt gagtgtctct tcggcctcat cgaccgtgag
     3901 cgaggttctg agcgccgcct ccacaggcgt gatgctgccg ccggcgtcca ctatggacat
     3961 gagcaattgt ttctctccgc ccgtcttcgg gccgcggggg cggcgtcctg tcagtagccg
     4021 catcaggtaa ccgaacaggg tgaacatggc gatcatgagc agcaacgtcg tggcgggttc
     4081 gggaccgcct gtaaggatga cgaacgacac taccaggcca atcatgaacc cgagcgccaa
     4141 caaggggtat ggattccctc tgaccgggcc ggcgggctct tgcggccacg tttcgtaccg
     4201 tcgttcccga aggtccacgc agcgcacgtc ccgcctccct cgttgttttt ccgaacagga
     4261 gccattatgt tgttcgggta tcaattgtag ggtaggaggg ttacagggag gtaacagtcg
     4321 atgacccttt ccgaagaggt ggcgtgggtt acgggaagca gcacggggat cgggcgcgcc
     4381 gtcgccgtcg agctcgcggc ggggggacgc agggtggtgg tccactacaa cgcgagcgag
     4441 gacgaggcgc gggaggtggt gaaggagatc gagggttacg gtggggaggc ttcgctcgtg
     4501 gggggcgatg tctccgacgc ggatgaggtc gggcgcatgg tcggggagat agagaaccgc
     4561 ttcgggcggc tcgacgtgct ggtcaacaac gccggatccc tgattgagcg gcgcgggatc
     4621 tccgcgatga cggaggatct ctgggaccgg gtcatggacg tcaacctgaa gagcgtctac
     4681 ctggtctcgc aggcggcgct cccgctgatg agagaggggg ggaggggcag gatcatcaac
     4741 ataacctctg tcgccgcccg caacggcggc ggtcccggct cggtcgccta cgccacggcc
     4801 aagggcggtg tcagcaccct tacccgggcc atggccaagg agctcgtctc cgagaacata
     4861 ctggtaaacg gggtagcccc aggcgtcata acgaccccct tccacgaccg tttcacgccc
     4921 cccgacgtgc gcgaaaacct caagagcgcc ataccgatgg gacgggaggg aaccccagag
     4981 gagacggccg gggtcgtcgc gttcctcgct tcccccgcgg ccgactacct ggtgggggag
     5041 atcatcgagg tcaacggcgg ccagcttatg agctgagacc tgccgatcgg ccttacggtg
     5101 gccccgggcg ggaagctgtg atcaccggcg tccgaagagt tgttctacgc gggcgctgac
     5161 ggcttcgccc agcttgcggt gctcgccggc gtcgaggtgg atgccgtcca cgtcgctcga
     5221 cataactacg gtggaggcgt cgaagaactc gcacccgtgc tgttcggcga agcgctcgta
     5281 gtgctcgggg aactgcctgg acttcccctc ggccccctcg aacatctggg ccatgtccgt
     5341 gagggtcgcg accggcgggg gagagatcag gagcaccacg ggggcgccgc cccccggccc
     5401 gcacccgctc ctcgttacca tctcggccag gccggcagct ccctgcgcga tgtccgaggc
     5461 cgagctgccg aagcggctct tcaggtcgtt ggtccctagc atgatcgtca ccaggtctac
     5521 cgggctgtgc gactcgagac atggccgcag gtaggtccta ccgttcttgt gcacgccctc
     5581 tatggggtcg tcccacacgg tcgtcctgcc ggacaatcct tcctcgatca ccctgtagtc
     5641 gtcgcctagc ctggccctga acacccccgg ccagcgcacg tcttcgggaa agcgctccct
     5701 ggtctccggg tcgaagcccc aggtgttgga gtccccgtaa caaagtatcg ttctcaccct
     5761 accccctcgc accggagatc gacgaagccc tctctgcaca cgaaacccat tgttggtctg
     5821 tcctccggcc cggtcaaccc cggccgacac ggcaaaccgc gggcgaacgt ggccgccata
     5881 tcgttaccga atggagagag aaccggggag gtcttgcggg tatgagcatc gtcaaggccc
     5941 cgggcacgct cgacgtggcg tccgtcgaac gcttgtgttt ccccaacgtg acccgggaca
     6001 gggtgacagc ggcgaacccc gccggccctc gaccggagtc cgtgctccgg aacgacccgt
     6061 cccggcgggc gagaacgggc tgacgccgcc ccggaaaact tgaaacacgt tgcactattc
     6121 agtacagtgt ttgcgcttcc aaaccagggt attaggttag tgtatgcttg acgattggct
     6181 gttcaaaggg gaccaacgga aggagacttg tggagcaaaa ttctgagggc ccgaggatcg
     6241 acagcaccgt attctgggtg gcggccgttc tctcggcggt attcgtagcc tgggggatat
     6301 tcttcacgga gagcctggca gccgtcttcg acgccgtgtt atggagcttt ctcgttccga
     6361 acttcggctg gatgttcata ctttcgacgt tcggcttcct atccttcgcg ctatatctgg
     6421 ccttcagccg gttcggtaag attcggctag gcgcccagga cgagcagccg gagttcagca
     6481 ccgtctcctg ggtggcgatg atgttcagcg cgggcatggg cataggcctc atgttcgacg
     6541 gcgtggcaga gcccctctcc cacatgggcg ctcccccgtt tggtctggca gaggccaaca
     6601 ccaaggctgg cgcccaggtc gcgatggagt actccttctt ccactgggct ttccacccgt
     6661 gggccatcta cgcgatcatg gggctggccc tcgcgtactt caccttccgc aagggga
//
LOCUS       DBNGVD010000471         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1390565, whole genome shotgun sequence.
ACCESSION   DBNGVD010000471 DBNGVD010000000
VERSION     DBNGVD010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1390565"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="AC16CM_12685"
     CDS             <1..928
                     /locus_tag="AC16CM_12685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR3140735.1"
                     /translation="AGRFGEYALGMPPSGAMDLFSYEVGNYLVGNREGEAGLEITYFG
                     PELTFTEDAVIAITGAEMPPKINGEEAATWETLAVSSGDVLTFDYLRSGARAYLTVAG
                     GIDVPLFMHSRSTYTLIGLGGHEGRALQEGDELKTGDAGDRADRVGKRVDEDHVPSYS
                     SETELRVIIGLASYRLTEESLEEFLNTTWTVTTDADRVGYRYRGGELGFVEREQPAGA
                     GADQANVVDFGYPIGSIQVPGGVEPIVLMNDAVTGGGYATIGTVISADRDKLAQTKTN
                     DRTRFRSVDLDEALEARKQRREQLEEIRQALQ"
     gene            1008..1781
                     /locus_tag="AC16CM_12690"
     CDS             1008..1781
                     /locus_tag="AC16CM_12690"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR3140736.1"
                     /translation="MQIDFNCDMGESFGPYSFGADEELMRYISSANVAGGFHAGDPHV
                     MRRTVELARENGVAVGVHNGYRDLVGFGRREIGVTPEEIQDELIYQLGALREFASLYG
                     MEVQHVKPHASLYMVAARDEEVSRAIIGAIQRVDPGLSIYCMHASVTYDLAREMDQPV
                     ITEFFADREYGDDGQIVFTRKVTEESDPDEVADRVVRGVTEGKVTTVNGKDIDIPSDS
                     VCVHSDTPGAARLAEAIVRKLAENDVEVRPPGRAGQRTA"
     gene            1794..2033
                     /locus_tag="AC16CM_12695"
     CDS             1794..2033
                     /locus_tag="AC16CM_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006067239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HIR3140737.1"
                     /translation="MADKTVRAQMPGTFYRRPDPESDFYAEEGDTVSAGDTVGLIEVM
                     KSFHEVKAEEDGTISRFLVENEDAVDAGQDLVELE"
     gene            2078..>3660
                     /locus_tag="AC16CM_12700"
     CDS             2078..>3660
                     /locus_tag="AC16CM_12700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3140738.1"
                     /translation="MTETARRYMGNSVPRKEDPALLTGRATFTDDIKLPGMLHLAVLR
                     SPHAHARIESIDVSAARNLPGVVEVFTGEDLAEEWAIGVPCGWPVTEDIKMPDHWPLA
                     RGEVNHVGEGVAVVVGEDRYRAQDALEHINVDYEPLPAVVDVEEALEDGSPLAHEEFG
                     TNECYTWPLATGDVDEAFEKADVVVKGRYLQQRLLPSAIEPRAVVATSEPASGGFVIY
                     TSTQVPHFVKDILSAICGIPDSRLRAVAPDVGGGFGSKLNVYAEEALALALTRRLGVP
                     IKWVEDRTENYISTIHGRGQVQHMELAATSEGKILGMKVRLLADMGAYLQLLTPGIAV
                     LGAFTYPGLYTFGAYSFECVGVFTNLTPTDAYRGAGRSEAAYAHERIMDDLARELGMD
                     PAELRLRNLMPAFDEPTMTPAGVMYDSGDYETCMRRALELAGYDELRAEQRRRREAGD
                     VKQLVIGIGNFTESGGLSPSKVAAGVRLQSGGWETATVRMRVSGKVEVVTGTSPHGQG
                     HVTAWSQIAADALGVDVDDV"
BASE COUNT          722 a         1167 c         1210 g          561 t
ORIGIN      
        1 cgccggacgt ttcggggagt acgccctggg gatgccaccg tctggggcga tggacctgtt
       61 ttcctacgag gttggcaatt atctggtcgg aaaccgggag ggtgaggccg ggttggagat
      121 cacctacttc ggccccgagc tgacgttcac cgaagacgcc gtcatcgcca taaccggcgc
      181 cgagatgccc ccgaagataa acggggaaga ggcggcgacc tgggagacgc tggccgtctc
      241 ctcgggcgac gtgctcacct tcgactacct gaggagcggg gcgcgggcct atctgaccgt
      301 ggccggcggt atagacgttc ccctcttcat gcacagccgc tcgacgtaca cccttatcgg
      361 gcttggcggc cacgaaggcc gcgccctgca agagggtgac gagctgaaga ccggcgatgc
      421 cggagaccgc gcagaccgcg ttggcaaacg cgtggacgaa gaccacgttc cgtcctactc
      481 tagcgagacg gagctacgcg tgatcatcgg cctcgccagc taccggctga ccgaagagag
      541 cctggaggag ttcctgaaca cgacctggac cgtaaccacg gatgccgacc gcgtcggata
      601 ccgttacagg ggcggcgagc tcggtttcgt cgagcgggaa cagccggccg gggcaggggc
      661 cgaccaggcc aacgtcgttg acttcggcta ccccataggt tccatccagg tacccggcgg
      721 cgtagagccc atcgtgctca tgaacgacgc cgtaaccggc ggcggatacg ccaccatagg
      781 aaccgttatc agcgccgacc gcgacaaact ggcccagacc aagaccaacg acaggacccg
      841 cttccgctcc gtagacctgg acgaagccct cgaagcccgc aaacaaaggc gggagcaact
      901 cgaggagatt cggcaggctc tccaatgagc gttcagcttt cagccttcag cttgtttggc
      961 tgactgctga aagctgacgg ctaaaggcgc cgaccgaagg gaggcacatg caaatagact
     1021 tcaactgcga catgggcgag agcttcgggc cctacagctt cggggccgat gaggagctca
     1081 tgcgctatat ctcgtctgcc aacgtggctg gcggtttcca tgcgggcgac ccgcacgtta
     1141 tgcgccggac cgtggagctg gccagggaga acggcgtggc ggtcggggtc cacaacgggt
     1201 atagagatct ggtcgggttc gggcgcaggg agataggcgt taccccggag gagatccagg
     1261 acgagctgat ctaccagcta ggggccctgc gcgagttcgc cagcctgtac ggtatggagg
     1321 tccagcacgt aaagccccac gcctcgctct acatggtggc agcccgcgat gaggaggtct
     1381 cccgcgccat cataggcgcc atccagaggg tagacccagg tctctccatc tactgcatgc
     1441 acgcctcggt tacctacgat ctcgcaagag agatggacca gcccgtcatc accgagttct
     1501 tcgccgaccg cgaatacggc gacgacggcc agatcgtctt tacccgcaag gtgacggagg
     1561 agtctgaccc cgacgaggtt gcagacaggg tcgtcagggg cgttacggag ggcaaggtca
     1621 ccaccgtgaa cggcaaggac atagacatcc cctccgactc ggtctgcgtc cacagcgaca
     1681 cccccggtgc ggcgaggctg gcggaggcaa tagtccgcaa gctcgcggag aacgacgtgg
     1741 aggtgcgccc gcccggacgc gccggacaga gaacagccta gaaggagaga aaagtggcag
     1801 acaagacggt cagagcgcag atgccaggca ccttctaccg tcgccccgac ccggagagcg
     1861 acttctacgc ggaggaaggg gacaccgtct cggctggcga cacggtgggc ctgatcgagg
     1921 tgatgaagtc ctttcacgag gtcaaagccg aagaagacgg caccatctcc aggttcctcg
     1981 tcgagaacga ggacgccgtg gatgccgggc aggatctggt agagctggag taacggcttt
     2041 cgcaaatagc gggcgcaacc ataaaggagg tcgccgcatg acggagacgg ctcgacgcta
     2101 catgggcaac agcgtcccac gcaaggagga cccggcgctc ctgacgggcc gcgccacctt
     2161 cacggacgac atcaagctgc cggggatgct gcacctcgcc gtcttgcgaa gcccacacgc
     2221 gcacgccagg atagagtcaa tcgacgtctc cgctgccagg aatcttcccg gtgtcgtgga
     2281 ggtcttcacg ggtgaggatc tggctgaaga gtgggccatc ggcgttccgt gcggctggcc
     2341 ggttaccgag gacatcaaga tgcccgatca ctggcccctg gcccgtggcg aggtgaacca
     2401 cgtgggcgag ggcgtggccg tggtcgtcgg ggaagaccgc tacagggccc aggacgccct
     2461 cgagcacata aacgtggact acgagccgct cccggccgtg gttgatgtgg aggaggcgct
     2521 cgaagacggc tcgccgctgg ctcacgagga gtttggcacc aacgagtgct atacctggcc
     2581 gctcgctacc ggggacgtcg atgaagcttt cgagaaggcc gacgtggtgg taaaagggcg
     2641 ctacctccag cagcgcctcc tcccaagcgc catagaacca agggccgttg ttgccacctc
     2701 cgaaccggca agcggcggct tcgttatata cacctcgacg caggtgcccc acttcgtcaa
     2761 ggacatcctc tccgccatct gcgggatacc cgactccagg ctccgggccg tcgcccccga
     2821 cgtgggcggc gggttcgggt ccaagctaaa cgtctacgcc gaggaggccc tggcgctggc
     2881 gcttacccgc cggctgggcg tgccgatcaa atgggtcgag gaccgtacgg agaactacat
     2941 ctcgaccata cacggccgcg ggcaggtcca gcacatggag ctcgccgcca cgagcgaggg
     3001 caagatcctg ggcatgaagg tcaggcttct ggcggacatg ggcgcatacc tgcagctcct
     3061 cacgcccggc atcgccgtgc tcggcgcctt cacgtaccct ggcctctaca cgttcggcgc
     3121 ctactccttc gaatgcgtcg gggtctttac caacctcacc ccgaccgacg cgtacagggg
     3181 cgccggccgc tcggaggcgg cctacgccca cgagcgaata atggacgacc tcgcccgcga
     3241 gctgggtatg gacccggccg aattgcggct gcggaacctg atgccagcct tcgacgagcc
     3301 gaccatgacc ccggccggcg taatgtacga ttctggagac tacgagacct gcatgcgccg
     3361 ggccctcgag ctagccggct acgacgagct ccgcgccgag cagagacgcc gccgcgaggc
     3421 cggcgacgtc aaacagctgg tcatcgggat cggcaacttt accgagagcg gcggcctctc
     3481 gccctccaag gtcgcggccg gcgtccgcct gcagagcggc ggctgggaga cggccaccgt
     3541 gaggatgcgc gtgtcgggca aggtggaggt cgttacaggc acctcccccc acggccaggg
     3601 ccacgtcacc gcctggtccc agatagccgc tgatgccctc ggggttgacg tggacgatgt
//
LOCUS       DBNGVD010000472         3078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2300849, whole genome shotgun sequence.
ACCESSION   DBNGVD010000472 DBNGVD010000000
VERSION     DBNGVD010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2300849"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2247
                     /locus_tag="AC16CM_12705"
     CDS             <1..2247
                     /locus_tag="AC16CM_12705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3140739.1"
                     /translation="LEHEMISVSSAEALGDVLHNESIDVILAGHTPDGRLALELARKA
                     RPGVPFIFISDTPGEEAVVEAMKSGAADYVLKHRSERLVPAVRRATRDGADRRGTASR
                     EDEQGYGAGEAAGRNEEWFRSLVRFASDVIMILDSEGVISYESPAVERILGFRLEDRV
                     GTEFLSHVHPEDLATAKRGLAEVLETPGCTLSVAYRARDGEGDWHQFEAYFTYLLDDP
                     MIRGIVVNARDSTELRHVEAALRESDERFRWTFEKAAENIFLVDLKSRRILDANATLQ
                     RSLGYTLEELKSMTLHDLVADEQESVGLNVEHVVAEGNTYVGERKYHRKDGSSIDVEI
                     NTGSIPFGDGQALCVVAHDITERKRTETELRQNLSVLLALREAGQVLSSTLESEEIVA
                     RLLEIMRSVAGLTAAVVSRFDQDGNLRVWRSAGLENLWPRVRYTSEAEGARRAALDNE
                     GRQLFWLQRPGGSADDYLAGLCLPLKTRKRTTGVLEAYGRESLADPDMVGIIDNLTSQ
                     AAAALENALLYEALGHRERALQNLVTKLLGSQEEERRRVSYEVHDGLAQVAVAAHQNL
                     QAFARRHAPDSEKGRRELELILKQVRATVSDARKVIANLRPTALDDLGLAAASSLEVE
                     RLDEEGYRVEYQDLLGDERLPAEIEITLFRVLQEALTNMRKHAQTEWVTIDLHRRNGE
                     VCLEVRDYGRGFDLAALGTWGAGPGERIGIAGMRERVGMLGGNLQIKSQPGAGTSVMA
                     SIPLSRDA"
     gene            2439..>3078
                     /locus_tag="AC16CM_12710"
     CDS             2439..>3078
                     /locus_tag="AC16CM_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140740.1"
                     /translation="MTSGNSREPTRLAIVDDHKLTRESLQNMLLDETDIEVVGEAENG
                     RQALLLCARLRPDLILMDVRMPEMDGLAATKEVKERYPETSVMMLTMHENPDYLLEAM
                     KAGAAGYVLKDAPQEEIIEAVRRIRNGESPLDPELAARLLRRLASEGEIRKGRDPRAD
                     APLTEPLTPRELEVLGLMKLGRTNRQIAADLVISLGTAKNHVEHIISKLGVSD"
BASE COUNT          643 a          935 c         1031 g          469 t
ORIGIN      
        1 ctcgagcacg agatgatcag cgtgagctcc gcggaagctc tcggagatgt gctgcataac
       61 gagagcatcg acgtcatcct cgcgggccac actccggacg gccgcctggc tctggaactg
      121 gccagaaagg cccggcccgg cgtgccgttc atctttatct ccgacacacc cggcgaagag
      181 gcggtcgtcg aagcgatgaa gagcggggcc gccgactacg ttctgaagca caggtcagag
      241 cggctcgtac cagcagtgcg acgcgccacc cgcgacggcg cagatcgacg gggaacagcg
      301 tcgcgagagg acgagcaggg gtacggggcc ggcgaagcgg cgggcagaaa cgaagagtgg
      361 ttccggtcgc tggtgcgatt tgcctctgac gtaatcatga tcctcgactc cgagggcgtc
      421 atatcttatg agagccctgc ggtggagcgg atactgggtt tccgtcttga ggacagggta
      481 ggcaccgaat tcctctcgca tgtacacccc gaagacctcg caaccgcgaa acgcgggctc
      541 gcggaggtcc tggagacgcc agggtgcacc ttgtccgtcg cataccgggc gcgcgatggg
      601 gagggcgact ggcaccagtt cgaagcctac ttcacgtacc tactcgacga ccccatgata
      661 cggggcatcg tcgtgaacgc ccgagattcg accgagcttc gccacgtcga agcggcgctg
      721 cgagagagcg acgagcgctt caggtggacc ttcgagaagg cggcggagaa catattcctc
      781 gtagatctga agagtaggcg catcctcgac gccaacgcga cgctgcagcg ttcgctcggc
      841 tataccctcg aagagctcaa gagcatgacg ctccacgacc ttgtcgccga tgagcaagag
      901 agcgtggggc tcaacgtgga gcacgtagtg gccgaaggca acacctatgt gggcgagagg
      961 aagtaccacc gcaaggatgg ctcctcgatc gacgtcgaga tcaacaccgg gtccatcccc
     1021 ttcggggacg gccaggcctt atgcgtcgtc gcccacgaca ttaccgagcg caagcgtacc
     1081 gagacggaat tgcgacagaa cctgagcgtg cttctcgctc tgcgcgaggc cggccaggtg
     1141 ttgagctcca cgctcgagtc ggaggagatc gtcgcgaggc tactcgagat catgcgcagc
     1201 gtggccggcc tcacggcggc ggtcgttagc aggttcgacc aggacggcaa cctgcgtgtc
     1261 tggcgatccg ccggcctcga aaacctctgg ccccgggttc gttacacgtc tgaggcggag
     1321 ggcgcccgca gggcggcgct cgacaacgag ggacgacagc tcttctggct gcagcggccg
     1381 ggtggttcgg cggacgatta cctcgccgga ctctgcctac cgctaaagac caggaaacgc
     1441 accaccggcg tgctggaggc atacggcagg gagtcgttgg cggaccctga catggtcggg
     1501 ataatagaca acctgaccag ccaggccgcg gccgccctcg agaacgccct tctgtacgaa
     1561 gcgcttggcc atcgcgaacg tgcgctgcaa aacctcgtaa cgaagctgct gggatcccag
     1621 gaagaagaga ggcggcgggt atcctacgag gtccacgacg gcctcgccca ggtcgcggta
     1681 gccgcccacc agaacctgca ggcgttcgcc cggcgccacg cccccgactc ggaaaagggc
     1741 aggagggagc ttgagctgat cctcaagcag gtcagggcga ctgtctccga cgcccgcaag
     1801 gtcatcgcca acctcaggcc caccgcactc gatgatcttg ggctcgcagc cgccagctct
     1861 ctggaggtcg aacgcctcga cgaggagggc taccgcgtcg aataccagga ccttctcgga
     1921 gacgagcgcc tgccagcgga gatcgagatc accctcttcc gcgttctgca ggaagcgttg
     1981 accaacatgc gcaagcacgc ccagacggaa tgggtaacca tcgatcttca tcggagaaac
     2041 ggggaagtct gtttggaggt ccgcgactac gggcgagggt tcgatctcgc cgcgctggga
     2101 acctgggggg ctggacctgg cgagcggatc ggaatcgcgg ggatgagaga acgggtgggc
     2161 atgctcggcg ggaaccttca gatcaagagc caacctggtg ccggcacgtc tgtgatggcc
     2221 agcatccctt tgtcgagaga cgcgtgacgg caggtcactg ccggtgggag gaacctgcag
     2281 gggggtgccg gcccgtccag atcggtgatc aggcggccgc agtagcatcc cgacgccggg
     2341 cacgaccaca cgcgagatcg aacacgagat cgcgaacact ttgaccgaac aagacaactg
     2401 ttggctgacg ggggttagta ggaatggtag aagctgaggt gacgtccggg aactcgcgcg
     2461 aacctacgcg cctggccata gtcgatgacc acaagctcac cagggagagc ctgcagaaca
     2521 tgctcctgga cgagacggac atcgaggtag ttggcgaggc ggaaaacggg cggcaggcgc
     2581 tcctgctctg cgcacgtctg aggccggatc tcatcctcat ggacgtaagg atgcccgaga
     2641 tggatggtct cgcggccacg aaggaagtca aggaaaggta cccagagacc agcgtcatga
     2701 tgctgaccat gcacgagaac cccgactacc tgctcgaggc tatgaaggcg ggagcggccg
     2761 gatacgtgct caaggacgcg ccccaggaag agatcatcga ggccgtgcgt cggatccgaa
     2821 acggcgagtc cccgctggac cccgagctgg cggcccggct tctgcgccgg ctagccagcg
     2881 agggcgagat acgcaaagga agggacccgc gggcggacgc ccctctgacg gagcctctga
     2941 ccccgcgtga gcttgaggtt cttgggctca tgaagttggg acgcaccaac cggcagatcg
     3001 ccgcagacct cgtgattagc ctcggtacgg cgaaaaacca tgtagagcac atcatctcca
     3061 agctgggggt ttccgacc
//
LOCUS       DBNGVD010000473         7835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_660455, whole genome shotgun sequence.
ACCESSION   DBNGVD010000473 DBNGVD010000000
VERSION     DBNGVD010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_660455"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..648)
                     /locus_tag="AC16CM_12715"
     CDS             complement(10..648)
                     /locus_tag="AC16CM_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140741.1"
                     /translation="MTREVKTQAQKSPLRREQAALTRTRIVEGATAVFELRGYEGARI
                     EDIAKEAGVAVPTVYKVFTNKRTLLRASVEAAITDGEAGEVERLAWFREQLEDPSAEG
                     QLRLIARNARRMYDRAGQLLEVVRAAAASDSDIEALLRAINDERLARARTSAERLASK
                     AKLRTTIGAAALTLWTLSVPVLYVLQIQVGGQIGVEYERWIGDLLVAALLDA"
     gene            765..1694
                     /locus_tag="AC16CM_12720"
     CDS             765..1694
                     /locus_tag="AC16CM_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3140742.1"
                     /translation="MVEQEATDTLEPLNLDESTVGRLLSMEEVIAAMERALADFSSGT
                     VIQPVRTMLPVAEHQGFLGLMPAYTGSALGAKLVAFYPRNTAVPTHHATILLFRPETG
                     EPLVSMDGRLITEVRTAAVSAVATDRLAKADASVLAIIGSGVQARSHLEALRLVRQFE
                     EVRVWSPRHAEDFARQFDGVGATSSAEEAVRGADVVVTATTSKTPVLSGEWLSPGAHI
                     NAIGGLAPWRELDDEALRRSRVYVDSREAAMKESGDVIAAGEIFAEVGEVVSGAKAGR
                     QSDEEVTLFKSLGLAVEDVATAELVYRKAQAGV"
     gene            2067..2495
                     /locus_tag="AC16CM_12725"
     CDS             2067..2495
                     /locus_tag="AC16CM_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140743.1"
                     /translation="MTRRTAPSMAMIAIAFLLASTLALALTMVGSAHERGISPSPADA
                     ASRVGVTAKCDGNPEKVIVKNNTRHRIKIKKVGSIYQPRSNEPFRVDRTLRRGASVTF
                     ESGFDANHNVLTRQYIFNSDVGSREGARVATSIGRYVDRC"
     gene            complement(2644..3858)
                     /locus_tag="AC16CM_12730"
     CDS             complement(2644..3858)
                     /locus_tag="AC16CM_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140744.1"
                     /translation="MLKTYRSLLSIPHARAFVVAGFVARLSISMRALGAVLMVSQLTG
                     SYGLAGAVAAAALLGEAVAAPRLGRLVDRYGQRTTLLVCLAVHSAGTLALVISAQFAA
                     PRWVLLATAALSGAAALQVGSLVRARWSALVGGSPALEPAFALESTLDELVFVMGPAL
                     VTALALGVAPGAGLLGALALTIVGSLALALQRRTEPEPAGVRDRPEGSAIGNQGLRVL
                     VATFVAVGAIFGTLDVAMVAFAEQVDSPGAAGPLLALAAAGSLFAGLAYGTRSWRWSL
                     GRRFVASVVVLWGGTVPLTIASSVALMAPAAILAGVAIAPTLIAGFTLVQRLVASGEL
                     TEGLNWTITALGAGAAIGAWTAGLIADSAGGQTAFLVAVIAGGVAVTVATCGRGNLLP
                     RRDEHEGPVQKS"
     gene            4224..4679
                     /locus_tag="AC16CM_12735"
     CDS             4224..4679
                     /locus_tag="AC16CM_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140745.1"
                     /translation="MSVETQAEVDFDRARRQAFYGRVAARLRRECNRLLAFDEVVDRC
                     SAHNRRRLGLRDVEVSKIVGSVGRTAAFDRAFMPTKASLKERWKRVDRAFHHGLDLPA
                     VRLYKLGDSYFVEDGNHRVSVARYQEVEMIEADVTEVYPLYLARSGCAA"
     gene            complement(4826..6694)
                     /locus_tag="AC16CM_12740"
     CDS             complement(4826..6694)
                     /locus_tag="AC16CM_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140746.1"
                     /translation="MRRIVYLLISAAAALLMASGVALAVVADVGGSDGTDDRVTSHTY
                     VRHDGGTDAGIRHCNSAASNSAEDNDTDDNDADSNDGGNLRQDNEPYSVVDPTDSNTV
                     VAGWNDYCLTDLGAGWEGFGFSRDGGETWTDSLVPGYPQDTSTEGRQSPLYGDHTDAG
                     DPIAAFDNDGRLFVGGISFNRDGAVNGDVFVASYGAQNQPNGYPVDYLRTRVVGKGTP
                     SRNFQGIFQDKPMLEVDRTGGPNDGNVYVCWSRFTAFGQNKIYFSHSENHGQTFSRPI
                     PLTNPGQIGSVQGCDIAVEEDGDVYVTYRTFSTGNRGSGLSFNRSTDGGESFTRPQRI
                     RNITEYFPSASGQRDCGDGDYLCPPSEFVFHRVPLEPRVTSDQSGELEGVYLTYNEIR
                     PGSIVPSDSTYSSAGAGRVGQSLVYVVSTTNDGRTWSSPTAVDPNTSGHQYFSDVDAF
                     SGEVAVVWQDSRTDPAYSVQRPIGNTAQATSSGTNIVNTFFAHSTDGQTWMDTTKVSS
                     QAHQPQYEMFGDRQIPFQGDYNWISLANSQTSEGSLFAYMSWADNRDVVPGADPREDE
                     QDGFDVLQCRTDLDTDTDGERGEGPLARRDPPFSGDNCANGGGLNQNIYGSSLELP"
     gene            complement(7102..7608)
                     /locus_tag="AC16CM_12745"
     CDS             complement(7102..7608)
                     /locus_tag="AC16CM_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HIR3140747.1"
                     /translation="MAQMINGRYTARTDEPFVVFMIGMRVNRPLQFRKWVPTARAMGP
                     MLKELYANPQKGFLGAETFVFWPGVALLQYWRSFEDLERFARSPSDPHLPAWQRFNKT
                     VGSDGSVGIFHETFIVERGNYEAIYSNMPVFGLARATEHVRAMGGRETARRRLMRGEN
                     EPAVPSPE"
BASE COUNT         1421 a         2450 c         2508 g         1456 t
ORIGIN      
        1 ccgaccgaat caggcgtcaa ggagggcggc taccaatagg tctccaatcc agcgttcata
       61 ttcgacccct atctggccac caacttggat ctgcaggacg tagagtaccg gtacgcttag
      121 ggtccaaagc gtcagagctg ccgccccaat cgtcgtcctt agcttcgcct tgctagctag
      181 gcgttcggca ctggtgcgtg cgcgggcgag gcgctcgtcg ttgatggccc gcaagagggc
      241 ctctatatcg ctatcactcg ccgcagcggc gcgtacgact tcgagcagtt gccccgcacg
      301 gtcgtacatt cgccgcgcgt tgcgagcgat gaggcgtagc tgtccctcgg cgctgggatc
      361 ttcgagctgc tcgcggaacc aggctagccg ctccacctcg cctgcttccc cgtcagtaat
      421 ggccgcctca accgaggccc tgagcagggt gcgcttgtta gtaaatacct tgtagacggt
      481 cggtacggcg acccctgcct ccttggcgat gtcctcgatg cgcgccccct cgtagcctcg
      541 tagctcgaac accgctgttg ctccctcgac gatacgagtg cgggtcagag ccgcctgttc
      601 gcggcgtagt ggggatttct gggcctgggt cttgacttcg cgggtcacta ggtgtagtgt
      661 accacaacat cgtatagtag aactatacaa aatatataag tactatcttt gggatagtgc
      721 tcacaaagag cctccggtaa ggagcgtggg gaaaggagca gtttatggtc gagcaggaag
      781 ctaccgatac ccttgagccg ttgaacctgg acgagagcac cgttggtcgg ctgctgagca
      841 tggaggaggt gatagccgcc atggagcgtg cactggccga cttctccagc ggcacggtga
      901 tacagccggt ccggacgatg ctacccgtgg ccgagcacca aggctttctc ggtctcatgc
      961 ctgcatacac cggttcggct ttgggcgcga agctggtcgc cttctaccct cgcaacaccg
     1021 ccgtgcccac ccaccatgcc acgatcctcc tgtttaggcc cgagaccggc gagccgctcg
     1081 tcagcatgga cgggcgcctt atcaccgagg tgcgcacggc ggcggtctcc gcggtggcca
     1141 ccgatcggct cgccaaggcc gacgcttcgg tgcttgcgat catcggctcc ggggtgcagg
     1201 cgcgcagcca cctggaggcc ttgaggctcg tacggcagtt cgaggaggtg cgcgtctgga
     1261 gcccacgcca cgccgaggac ttcgctcggc agttcgacgg tgttggcgcg acatcttcgg
     1321 ctgaggaggc ggtgcgaggc gccgacgttg tggtgaccgc gacgacgtcg aagacgcccg
     1381 tgctctcggg agagtggctc tcgccgggag cgcacatcaa tgcgataggt ggtctcgctc
     1441 cttggcgaga gctcgacgac gaggctctgc gccgctctcg ggtttacgtg gactctcggg
     1501 aggccgctat gaaggagtcg ggtgatgtca tcgccgcggg agagatcttc gcagaggttg
     1561 gggaggtcgt ttctggagcg aaggccggtc ggcagtctga tgaggaggtg acactcttca
     1621 agtcgcttgg gctagcggta gaggacgtgg cgacggcgga gctggtctac cgcaaggccc
     1681 aagccggcgt gtagcggctg tcgtccttgt tgcctttatt cacccaacct cgtagaaggg
     1741 gtattctccg aaacttgtat gcaggatcgt gcatagaccg accgcaggcg ccgcgaagag
     1801 cgcacctcaa ggccgccgcg atccggtaca gtaagactac atgttgtggt ggcgcatgga
     1861 tatgcgggag agcgaatagt cccgccctgg gtccccaagc ccgcctcacg ggcggagcta
     1921 tccggtagcg tctgctgcgg ccccagaaat gtccacctgg catatacgcg cctcccgcgg
     1981 ccgtggggta ggctcgggtc atgttgacta ctggggcggg tgaccgaacg tatcctctcg
     2041 ccctccgaga tggggaggac taccgcatga cgagacgcac ggctccgtcc atggccatga
     2101 tcgcgatagc ttttttgctg gctagcaccc tcgccttggc gctgacgatg gtgggctccg
     2161 cacacgagcg cggcatctcc ccctccccgg cggacgccgc ctcgcgggtt ggtgtcacgg
     2221 ccaagtgcga cggcaacccc gagaaggtca tcgtaaagaa caacacgcgc caccgcatca
     2281 agatcaagaa ggtcggatcg atctaccagc cccgctccaa cgagcccttc cgcgtggacc
     2341 gcacgctgcg caggggcgcg tcggtaacct tcgagagcgg cttcgacgcc aaccacaacg
     2401 ttctcacccg ccagtacatc ttcaactccg acgtaggctc cagggagggc gcgagggtgg
     2461 ccacctccat cggccgctac gtggaccgct gctaaccagg gaggagcggg tgggggtagc
     2521 gtcgcctact cccgccccct gatgcggcct cgcttctcca ggagcgccaa tcgacgtgct
     2581 gcctggagag aaagcttgcg tgggcccaca cgaaggcggt aggagcgatc ccacggacgc
     2641 ctgctacgat ttctgcaccg gtccttcgtg ttcgtccctg cgggggagca gattgccccg
     2701 cccgcacgtt gccaccgtta cggcgacgcc gcccgcgatc accgcgacca agaaggcggt
     2761 ttggcccccc gcactatcgg cgatcagccc cgccgtccat gcccctatcg ccgcccctgc
     2821 gcccagcgca gtgatcgtcc aattcaaccc ttcggtaagc tcccccgaag cgaccaaccg
     2881 ctgcacgagg gtgaacccag cgatgagcgt aggcgctatc gccacgcccg ccaagatggc
     2941 ggctggcgcc attagcgcga cgctagacgc aattgtgagc gggaccgttc cgccccagag
     3001 tacgaccacc gacgctacga agcgcctgcc cagcgaccat cgccagctgc gagtgccgta
     3061 ggccaagcca gcaaagaggc tgcctgcagc ggcaagcgcg agcagcggac cggccgcgcc
     3121 gggagagtct acttgctcgg cgaacgcgac catcgccacg tccaaggtcc cgaagatagc
     3181 tcccacggcg acgaaggttg ccaccagcac ccgcagtccc tgattaccta tggctgatcc
     3241 ctccggccgg tcgcgtacgc cggcgggttc cggttcggtg cggcgttgga gcgccagcgc
     3301 aagcgacccg acgatagtga gcgcgagggc gccgagcaac cccgcgccgg gggcgacccc
     3361 gagtgccaag gcggtgacaa gtgctggtcc catcacgaag acgagctcgt cgagggtaga
     3421 ctccagcgcg aacgctggct ccagggcggg cgagcctccc accagggccg accaccgggc
     3481 ccgcaccagc gaccccacct gcagcgccgc cgccccggac agggccgctg tcgccaacag
     3541 cacccacctt ggtgccgcga actgcgctga aatcacgagg gcgagcgtac ccgcggagtg
     3601 gacagcgagg cacacgagca acgtcgtccg ctgtccgtag cggtccacca accggccgag
     3661 ccggggagcg gccaccgctt cgccgagcag ggccgccgcg gcgacggctc cggccagccc
     3721 gtaagaaccg gtgagctggg agaccatcaa cacggcaccc aaggcccgca tggatatgga
     3781 aagtcgtgcc acgaaccccg cgacgacgaa agcgcgggca tggggtatgg agagcaacga
     3841 acggtaggtc ttgagcacgc ccactacctt accgtgtcat gcaatccatg tcccgtgaaa
     3901 cacccgcgtc cgcgatgatg cgccgtcgag acccgcgtgt aagctagacg gtaagcctgt
     3961 tttcatggcc tgcgtcggtc gacgcggtgt cggtggcgac gccggcgccc gtagacccgc
     4021 ggctgggtac aacgcagaag ggtagcgaat cggtgcggtg aagacttcgg gaattggtgg
     4081 ctcttccgtc caggtcaccg ctaccctttc ttggactttc gtcatgaatc aggaacgatc
     4141 gcgaccggtt aggcccgtac catcaagcca ctagtcaagt acgtgttggt ggcaagaggg
     4201 gcggctaggc gatgatcgta tttatgagcg ttgaaacaca ggccgaagtg gacttcgacc
     4261 gcgcccgtcg tcaggccttc tacgggcgcg tggctgcccg tttgcggcgc gaatgcaatc
     4321 gactgctggc cttcgatgag gttgtggata gatgctcggc gcacaacagg cgccgcctgg
     4381 gcctgaggga cgtcgaggtc tcgaagatag tcggtagcgt tggccgcacc gcggccttcg
     4441 atcgcgcctt catgcccacg aaggcgagcc tgaaggaaag gtggaagagg gtggaccggg
     4501 cgttccacca tgggctagat ctaccggcag tcaggttgta caagttgggc gactcctact
     4561 tcgtcgaaga cggtaatcac cgtgtcagcg tcgcccgtta ccaggaagtg gagatgatcg
     4621 aggcggacgt aaccgaggtc tatccgctct accttgcacg ctcggggtgt gcggcgtagg
     4681 ctgcatgtta ggcgaaaccg tcgccccgag tttgtgccag caagtccccg ccaaagggct
     4741 tgccaacctc catcggtggc ggtcagaacg ttggtggccc tgaccgtcag ggccaccaac
     4801 cagtgtctct ctagtgcaca gagccctacg gaagttccaa ggagctcccg tagatgtttt
     4861 ggttcagacc accgccgttg gcgcagttgt cgccactgaa tggcggatct ctgcgggcaa
     4921 gcggcccttc gcctctctca ccgtcggtgt cggtgtccaa gtcggtgcgg cactgcaaga
     4981 catcgaagcc gtcctgctca tcctcgcgtg ggtccgcgcc aggaacgacg tcacggttat
     5041 ccgcccagct catgtaggcg aagagagaac cctcggaagt ttgcgagttg gccagggaga
     5101 tccagttgta gtctccctgg aaggggatct ggcgatcccc gaacatctcg tactgcggtt
     5161 gatgagcctg gctcgatacc ttcgtcgtat ccatccacgt ctggccgtcc gtggagtgcg
     5221 cgaagaaagt gttcacgatg ttggtgccgg acgaggtggc ctgcgcggtg tttccgattg
     5281 ggcgctgcac ggagtaggcg gggtcggtcc ggctgtcctg ccagacgacg gccacctctc
     5341 cgctgaaggc gtcgacgtcg gagaagtatt ggtggccgct cgtattagga tctactgcag
     5401 ttggtgaact ccaggtcctg ccgtcgttcg tggtgctgac gacgtagacg agcgactgcc
     5461 ccacccttcc ggcaccggcc gagctgtaag tgctgtcgct cggcacgatg cttccgggcc
     5521 ggatctcgtt gtaggtgagg tagacccctt cgagctcgcc gctctggtcg gaggtgacgc
     5581 gcggctcaag cggcacgcgg tggaatacga actccgacgg agggcacagg tagtcgccgt
     5641 cgccgcagtc gcgctgcccg ctagcggagg ggaaatactc ggtgatgttg cgtatgcgct
     5701 gcggcctggt gaagctctct ccgccatccg ttgagcggtt gaaggagagc ccgctgccac
     5761 ggttaccggt ggagaaggtt cggtaggtga cgtaaacgtc gccgtcttcc tccacggcaa
     5821 tgtcgcatcc ctgtactgag cctatctgtc ccggattggt gagggggatc ggccgggaga
     5881 aggtttgtcc gtggttctcc gagtggctga agtagatctt gttctgcccg aaggcggtaa
     5941 aacgcgacca gcaaacgtag acgttgccgt cgttgggacc gccagtccga tctacttcga
     6001 gcatcggctt gtcctggaag atcccctgga agttgcgcga tggcgtccct ttacccacga
     6061 cacgggtgcg caggtagtcg acgggatagc cgttaggttg gttttgggct ccgtaggagg
     6121 ccacgaagac gtcgccgttg acggcgccat cccggttgaa ggagatgccg cccacgaaga
     6181 gcctgccgtc gttgtcgaag gcggctatcg ggtccccggc gtcggtatga tctccataca
     6241 ggggagactg gcgtccctca gtcgaggtat cctgaggata gcccggaacc agggaatcgg
     6301 tccacgtctc cccaccgtca cgggagaaac cgaagccctc ccatcctgcg ccaaggtcgg
     6361 tcagacagta gtcgttccag ccagccacta ccgtgttaga gtccgtcgga tcaacgaccg
     6421 agtacggctc gttgtcctgc cggaggttgc ccccgtcgtt tgagtctgca tcgttgtcat
     6481 ctgtgtcgtt gtcttctgct gagttggagg cagcactgtt gcagtgcctg atccccgcat
     6541 ccgtgccgcc atcatgacgc acgtaagtgt ggctggtgac cctgtcatcg gtgccgtcgg
     6601 aaccgccaac gtccgccacg acggccagag ccactccgct tgccatgagg agcgcggccg
     6661 cagccgaaat caataggtaa acaatgcgtc tcaagatgct tctcctttcc ctacttttcc
     6721 tcttgcagag gaatggagga aaagtccccc gatcctgctg ctcgatggaa gaccataata
     6781 gactgcgcta gcgtcttgcg catccctttg gggatggggc ctcaagattt atttacatcg
     6841 gcgtggcccg tggcgcccgc gaggaccgga gtctcctcgg attcaggggt aggcgaccgc
     6901 acattcgctt tgcagaatga tgaatgtagg gggcagactg tagtgtctgt cccctcatcc
     6961 cttgtctatg tgacgagtcg tacgctcatc gcgccttcgg aatcgagcct aaccgcgaag
     7021 gacggcgctg ttgtcgtaat cggcgtcggc cgacgcggag acaaggcatg gccagaagcg
     7081 gccaatttgg cgcgatcgtg gctactctgg agaaggcacg gccggttcgt tctcgccgcg
     7141 catcaggcgt ctcctggccg tctcccgtcc acccatggca cggacatgct ctgtggccct
     7201 ggccagcccg aagacgggca tgttggagta gatggcctcg tagtttccgc gctccacgat
     7261 gaaggtttcg tggaagatcc ccacgctacc gtccgaacca accgtcttgt tgaacctctg
     7321 ccacgcaggc aggtgcgggt cggacggact tctcgcaaac ctctccaggt cctcgaacga
     7381 gcgccagtac tgaaggagcg ccacgccggg ccagaagacg aaggtctctg ctccgagaaa
     7441 acccttctgc gggttggcgt acagctcttt gagcatgggg cccatcgctc gcgccgtggg
     7501 gacccacttg cggaactgaa ggggccggtt cacgcgcatc ccgatcatga aaacgacgaa
     7561 gggctcgtcc gtgcgcgccg tgtacctgcc gttgatcatc tgtgccatgc caaccttcct
     7621 ttcgtccaga ctcggcgaag gggtgctgat ccccgacggc tcacgagatg cgaggctttc
     7681 gagtctctcc tcgacccacc tgccggtctc tcggtttcct gataagtcct atacccagaa
     7741 cgaagacacc cgaggccaac agcagggcgc ccacgtaggc gaggtagatc atggcgttcg
     7801 gctccgtagc ttccggtgag agcacggaga gaaag
//
LOCUS       DBNGVD010000474         2451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_297939, whole genome shotgun sequence.
ACCESSION   DBNGVD010000474 DBNGVD010000000
VERSION     DBNGVD010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_297939"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16CM_12750"
     CDS             <1..652
                     /locus_tag="AC16CM_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3140748.1"
                     /translation="DFTRDLERGVRGGVIGLPASFYFEHLDGEVARRVEEAVELFSSL
                     GAEVREVEIPHLWDTLHAQRLILAAEAYAVHEERLQTEPESFDDQGLERLLDGENLRA
                     YRYANARQSGLRSGMEFAEVLRAVDVLLTPTVPIPAPEIGRRETMIDGYEEAVYSALT
                     RLTGPTDLNGLPSLSVPCGTTATGLPVGLHLIGRSFDEATLYRFGHAHEQAAGPDR"
     gene            729..2171
                     /locus_tag="AC16CM_12755"
     CDS             729..2171
                     /locus_tag="AC16CM_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-cytosine permease family protein"
                     /protein_id="HIR3140749.1"
                     /translation="MEEREGLGRQIERRSIDYVPEDERHGKVWHQGPFWFLGNFQFFT
                     ITIGFIGPGLGLSLGWTILAGTLGILFGTLFMAFHAIQGAQLGLPQMVQSRAQLGYRG
                     VLLALVATFFTFVAFNVVDSVLISAGLNGLFGWNGTVVIVAIAAVSALFAIYGHDYLH
                     TLFRVLFYVSLPLYVILTLAILLGGAGGDGSSPGGFVLVAFMAQFTASASYNITYAPY
                     VSDYSRYLPRHTRPGYIIAAVFFGASGSAVWLIALGAWLATRLGATDALVSLRDAGDA
                     VFGGLGVVLALSSVAALVATMGINAYGAMLTTVTAVDAFRKVTPTRRLRVVTITALAV
                     LWVVVSLSISARAVDVLFAALIMMLYLLAPWTAINLIDYFFVRHGHYAITDLFVPDGL
                     YGTWAWRGITAFLLGFAASIPFWVLPGIWTAPLGNILQGIDVAWLVGLLVSGASYYAL
                     SRSLDVDAEMEEVRASEQELEGDPTGAPSV"
     gene            complement(2189..>2451)
                     /locus_tag="AC16CM_12760"
     CDS             complement(2189..>2451)
                     /locus_tag="AC16CM_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3140750.1"
                     /translation="AQVPGDVEVEAVVLSGRDWEHALDSLGWEEGEVLIVGSSRLGPV
                     ARLFLGSNSTKIIRSSPVPVLVIPRGADVHLGEDASTGSRGI"
BASE COUNT          376 a          840 c          783 g          452 t
ORIGIN      
        1 ggactttacg cgggacctcg aacgcggcgt gcggggagga gtgataggcc ttcccgcaag
       61 cttctacttc gagcacctcg acggtgaggt ggccagacgg gtagaagagg cggtcgaact
      121 attctcctct ctcggcgcgg aggtacggga ggtggagatc ccccacctct gggacacact
      181 gcacgcccag aggctcatcc tcgccgcgga agcgtacgcc gtccacgagg agcgcctcca
      241 gacggaaccc gaaagtttcg acgaccaggg actggaacgt ctcctggacg gcgagaacct
      301 gagggcctac aggtacgcca acgcgcggca gagcgggctc cgctccggga tggagttcgc
      361 ggaagtcttg cgggcggtcg acgtgctcct cacgccgacc gtaccgatcc cggcgccgga
      421 gatcgggcgg cgcgagacca tgatcgacgg ttacgaggag gcggtctact cggccctcac
      481 gcgcctcacc ggcccgacgg acctgaacgg actgcccagc ctctcggttc cctgcggcac
      541 cacggccacc ggcctacccg tggggctcca cctcatcggc cggtccttcg atgaggccac
      601 cctctaccgc ttcggccacg cccacgaaca ggcggcaggt ccggatcgtt gaccggcgct
      661 ccttcggaat ctagactggg tccgcaaggt gtagacgaga tgtcgaggag gggtgggtgg
      721 ccgccggcat ggaggagcga gaggggcttg ggcggcagat cgagcgccgt tccatcgact
      781 acgtgccaga ggacgagcgg cacggcaagg tgtggcacca gggacccttc tggttcctgg
      841 gcaacttcca gttctttacg ataacgatcg ggtttatcgg gcctggcctt ggactatccc
      901 tgggatggac tattctggcc ggcaccctgg gcatcctgtt cgggacgctg ttcatggcgt
      961 tccacgccat ccagggcgcg caactcgggc tgccacagat ggtccagtcg cgggcgcagc
     1021 tcggctatcg cggggtgctg ctcgccctgg tggcgacgtt ctttaccttc gtggcgttca
     1081 acgtggtcga ctcggtgctc atctcggccg ggctgaacgg cctcttcggc tggaacggga
     1141 cggtcgtgat agtggctatc gccgccgtct cggcgctctt cgcgatctac ggccacgatt
     1201 acctgcacac cctgttcagg gtcctcttct acgtctcctt gccgctgtac gttatcctta
     1261 cgctggccat cttgctgggc ggcgccggcg gtgacgggtc gagccccggc gggttcgttc
     1321 tcgtcgcgtt catggcgcag ttcacggcga gcgcctcgta caacatcacc tacgcgccat
     1381 acgtctcgga ttactcgagg tacctgccac gccacaccag gccgggctac atcatcgcgg
     1441 ccgtcttctt cggggcctct ggctcggcgg tctggctcat cgcgctcggg gcctggctgg
     1501 cgaccaggct tggcgccacc gacgcgcttg tctccctgcg cgacgcaggc gacgccgtct
     1561 tcggggggct cggggtggtg ctcgcgctct cctcggtggc cgcgctcgtg gctaccatgg
     1621 gtatcaacgc ctacggcgcc atgctcacga ccgtcaccgc cgtggacgct ttcaggaagg
     1681 tgaccccaac caggaggctg cgggtcgtta ccatcaccgc gctcgccgta ctctgggtcg
     1741 tggtctcctt gagcatctcg gcaagggccg tagacgtgct cttcgcggcc ctgataatga
     1801 tgctctacct gctcgcccca tggaccgcga tcaacctcat agactacttc ttcgtcaggc
     1861 acggccacta cgcgataacc gacctcttcg tccccgacgg cctctacgga acctgggcct
     1921 ggcgcggcat aactgccttc cttctcggct tcgccgcgag catccccttc tgggtgctac
     1981 ccggcatctg gacggccccg cttggcaaca tcctccaggg tatagatgtg gcctggctcg
     2041 tcggcctcct cgtctcggga gccagctact acgccctcag ccgctccctc gacgtcgatg
     2101 ccgagatgga ggaggtgcgc gccagcgagc aagagctcga gggcgacccc accggcgccc
     2161 cctccgtgtg atcctcggga ccgagggatt atatccctcg cgaacccgtg gatgcgtctt
     2221 cccccagatg cacgtcggcg ccgcggggta tgacgagcac ggggacgggt gagctgcgga
     2281 tgatcttggt ggagttcgag cccagaaaca gcctcgccac gggtccgagg cggctagatc
     2341 ccacgatcag cacctcgccc tcctcccacc ccaggctgtc cagcgcgtgc tcccaatcgc
     2401 gaccactcag gactacggcc tccacctcga cgtcgccagg cacctgggcg a
//
LOCUS       DBNGVD010000475         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3030672, whole genome shotgun sequence.
ACCESSION   DBNGVD010000475 DBNGVD010000000
VERSION     DBNGVD010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3030672"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="AC16CM_12765"
     CDS             complement(<1..1144)
                     /locus_tag="AC16CM_12765"
                     /EC_number="4.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016841 - ammonia-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0170035 - L-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAL/PAL/TAL family ammonia-lyase"
                     /protein_id="HIR3140751.1"
                     /translation="MTLSDQTTGTVTLTGDDLSIEEVVRIARRGARVEISREALDRVR
                     RARDVVDRVLERGDLVYGMNTGVGSLSRYRIPFENLEQFSVRLVTRHTTHQGSELNAS
                     VVRAMMATRANGMAKGGVGVRAELIQAFLDALNAGVHPVVRTGGSIGQADLAEMAEVG
                     QVLIGRGEADFEGRRLPGAEALHAAGLEPVHLKAKEGLALISANGVTMGHGSLVLADV
                     ADLLDAFDVTAALSLEAYGANLSIIHPEATRMRPHPGQSRAADRLRELLDGSYLWRPG
                     AARNLQDPLSFRCVPQTHGACYDAHAYSRGTMEVELNSASDNPLVLLDDQSIISVGNF
                     DVVALAVAFDLLRVAVAQVVHLANERIQKHLWSQFSGLPTGLAASDG"
     gene            1300..2967
                     /gene="hutU"
                     /locus_tag="AC16CM_12770"
     CDS             1300..2967
                     /gene="hutU"
                     /locus_tag="AC16CM_12770"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR3140752.1"
                     /translation="MADIKKAPTIRAPRGRDLTCKGWHQEAALRMLMNNLDPEVAERP
                     EDLVVYGGTGKAARNWSSFWAIVGTLQGLEDDETLLVQSGKPVAVFRTQPWAPRVLIA
                     NSLLVPEWADWETFRELERAGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAEQRFG
                     GTLRGRVCLTAGLGGMGGAQPLAITMNRGVALCVEVDADRIERRIAHRYLGERIDDLD
                     AAVERAEEAKRAGEPLSIGILGNASEVFPALLERGYIPDAVTDQTSAHDPLVGYIPAG
                     YDLEAAAELREDDPARYITEARASMARHCAAMVGFKERGAEVFDYGNSLRGEAKLGGF
                     DGAYAYPGFVPAYIRPLFCEGKGPFRWAALSGEESDIAATDDAILDLFPDDARLGRWI
                     SEAREKVHFQGLPARICWLGAGERHRAGLRFNELVADGSISAPIVIGRDHLDTGSVAS
                     PYRETEAMKDGSDAIADWPILNAMLNVAGGASWVAVHHGGGVGIGRSIHAGAQVLADG
                     TKDGAARLERVLFNDPGIGVARHADAGYEAARTAARRLGIQMPMVDG"
     gene            3061..>3315
                     /locus_tag="AC16CM_12775"
     CDS             3061..>3315
                     /locus_tag="AC16CM_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIR3140753.1"
                     /translation="MIPDLVIDAHGVRSDVGVTIEDGSIAEVGPAREGERLSGIALAP
                     GFVNDHSHAFQRGLRGSVEQIDPSRPRDDFWTWRERMYRLA"
BASE COUNT          558 a         1081 c         1137 g          539 t
ORIGIN      
        1 cgccatcgct ggcggcgagg ccggtgggca ggccggagaa ctgggaccag aggtgcttct
       61 ggatacgctc gttggcgagg tgaacgacct gggcgacggc gacgcgcagg aggtcgaagg
      121 ccacggcgag ggcgaccaca tcaaagttgc cgacggagat gatggactga tcgtctagca
      181 ggaccagcgg gttgtcgctc gccgagttga gctcgacctc catcgtgcct cgggaatagg
      241 cgtgggcgtc gtagcaggcg ccgtgggtct gtggcacgca gcggaaagag agtgggtctt
      301 gcaggttgcg ggccgcgccg ggtcgccaca ggtagctgcc gtcgagcagc tctctgagcc
      361 tgtccgccgc gcgcgactgg cccgggtggg gccgcatcct ggtcgcctcg gggtgaatga
      421 tcgacaggtt tgccccgtag gcctccaggg agagcgccgc tgttacgtcg aaggcgtcca
      481 gcaggtcggc cacgtcggcg agcaccagcg agccgtggcc catggtcacc ccgttggcgc
      541 ttatcagggc gaggccctcc tttgccttca ggtggacggg ctccagcccg gcggcatgaa
      601 gagcctctgc accaggcagc cggcgtccct cgaagtcggc ctctccgcgg ccgatcagga
      661 cctgcccaac ctccgccatc tcggccaggt cggcctgacc aatcgaaccg ccggtacgga
      721 ccacggggtg gaccccggcg ttcagggcgt cgagaaaagc ctggatcagc tcggccctaa
      781 cgcccacgcc acctttggcc atgccgttcg cccgcgtggc catcatggcg cgcaccacgc
      841 tggcgttcaa ctcggagccc tggtgggtgg tgtgtcgggt aacgaggcga accgaaaact
      901 gctccaggtt ctcgaacggg atgcggtagc gggagagcga gcccacgccc gtgttcatgc
      961 cgtagaccag gtcaccgcgc tccagcaccc tgtctaccac gtcccgcgca cgcctcacgc
     1021 ggtcgagcgc ctcgcgggag atctccacac gtgccccgcg gcgcgctatt cggaccacct
     1081 cctcgatgga gaggtcgtcg ccggtcaacg tcaccgtgcc cgttgtctga tcggagagcg
     1141 tcaagcgtgt cctttggtcc ccgtcatatt actcccacct cctggacttt tggagggccc
     1201 gatgccagac tggatcttgt gagctatcag ctttcagcct ttagcaagaa caaaaagctg
     1261 aaaacgtggt tcgcaaagcg ggcggctcga ggaggtccca tggcagatat caagaaagct
     1321 cccaccatca gggcgccaag ggggcgggat ctcacctgca agggctggca ccaggaggca
     1381 gccctgcgca tgctaatgaa caacctcgac cccgaggttg ccgagcgtcc ggaagacctc
     1441 gtggtctacg gtgggacggg caaggcggcc cgcaactggt ccagcttctg ggccatcgtc
     1501 ggtaccttgc agggcctgga agacgacgaa acactgctcg tccagtccgg caagcccgtg
     1561 gctgtcttca ggacacaacc ctgggccccg cgggtcctga tcgccaactc cctgctcgtc
     1621 ccggagtggg ccgactggga gacgttcagg gagctggagc gggccggcct gaccatgtac
     1681 gggcagatga ccgccgggtc ctggatctac atcggaaccc agggcatctt gcagggcacc
     1741 tacgagacgt tcgccgcggt ggccgaacag cgcttcgggg gaaccttgcg gggccgcgtc
     1801 tgcctcaccg ccggcctcgg cggcatgggg ggtgcccagc cgctggcaat caccatgaac
     1861 cggggagtag ccctctgcgt cgaggtcgat gccgacagga tcgaacgccg tatagcccac
     1921 cgctacctgg gcgagcgcat cgacgacctg gatgcggccg tggagcgcgc ggaagaggcg
     1981 aagcgggcag gggagccgct ctccatcggc atcctaggca acgcgtccga ggtctttccc
     2041 gcgctgctgg agcgcggcta catccccgac gccgtcacgg accagacctc ggcccacgac
     2101 ccgctcgtgg gctacattcc agccggatac gacctggagg cggccgcgga gttgcgcgag
     2161 gacgacccgg cccgctacat cacggaggcc cgcgcctcga tggcccgcca ctgcgccgcg
     2221 atggttggtt tcaaggagcg tggcgccgag gtcttcgact acggcaactc gctgcgcggg
     2281 gaggcgaagc tcggtggttt cgatggtgcc tacgcctacc caggctttgt tcccgcttac
     2341 ataaggccgc tcttctgtga gggcaaaggt cccttccgtt gggccgcgct ctccggtgag
     2401 gagtcggaca tagcggcgac tgacgacgcc atcctggacc tcttccccga cgacgcacgg
     2461 ctggggcgtt ggatctccga ggcgcgcgag aaggtccact tccagggcct gcccgcccgc
     2521 atctgctggc tcggagcggg agagagacac cgagccggcc tgcgcttcaa cgagctcgtc
     2581 gccgacgggt ccatctccgc acccatcgtt ataggccgag atcatctgga caccggaagc
     2641 gtcgcctcgc cctaccgcga gacggaggcg atgaaggacg gctccgacgc catcgcggac
     2701 tggcccatcc tgaacgccat gctcaacgtg gccggcgggg caagctgggt tgccgtgcac
     2761 cacggtggcg gcgtggggat cggacgttcc atccatgccg gggcccaggt cctcgccgac
     2821 ggcacgaaag atggcgccgc ccgcctggag cgcgtgctgt tcaacgaccc gggtatcggc
     2881 gtcgcccgtc atgccgacgc cggctatgag gcggccagga ccgccgccag acggctgggt
     2941 atccagatgc cgatggtgga tggataaggc tacaggctac gggtttcagg tttcaggttg
     3001 ggccggtagc ggatctctga cggttcgcgc gaccagacgc cttgtgacgt gaggcgtacc
     3061 ttgatccctg atctcgtaat cgacgcgcac ggcgtgcgga gcgacgttgg cgtgacgatc
     3121 gaggacggta gtatcgccga ggtcggaccg gccagggagg gcgaacgctt gtccggcatt
     3181 gcgctggcac ccgggttcgt caacgaccac agccacgctt ttcagcgggg gttgaggggc
     3241 tcggtcgaac agatagaccc atcgcgtccc cgcgatgact tctggacctg gcgcgagcgg
     3301 atgtacaggc ttgcc
//
LOCUS       DBNGVD010000476        10314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4671469, whole genome shotgun sequence.
ACCESSION   DBNGVD010000476 DBNGVD010000000
VERSION     DBNGVD010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4671469"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..523
                     /locus_tag="AC16CM_12780"
     CDS             374..523
                     /locus_tag="AC16CM_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140754.1"
                     /translation="MTISYRTGLVGEPPPTEQPQSKADCRKGGYEEYGFKNQGLCIKG
                     VNHPR"
     gene            779..2050
                     /locus_tag="AC16CM_12785"
     CDS             779..2050
                     /locus_tag="AC16CM_12785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3140755.1"
                     /translation="MFVRNMHLSMWTAAFLVIGSVLLLGSGCGGGDGGEGGSSTGTQN
                     NKGGAQATEDDLNAALEKSFKETDAPGVVAAVETPDYTWVQAKGVADRASGEQMTPEV
                     HHRIGSVTKTFTATLLLKAADDGLLSLDDTIDQYVKGVPNGDEITLRQVADMTSGIAS
                     YTESDQWVEEWSSNPNKVWKPEDLAQYGIEESPLFKSGTAWFYSNTNYVLLGLVLQQV
                     TGKPIEQLYEEEIIRPLDLTETSFPGKSSAIPEPYDHGYTLQGKSSGQKPIDSTHWSP
                     SMAWTAGEMISTVDDLLVYGRALGTGKGLLSPEMQSERLDSFVSDVPPLNQPPLKGDL
                     EYGIGLGKDHGWVGHNGEIPGYNTYLFYHPDLDAVVVVLVNSDISSGKCPKDVPIMKA
                     WPRNASCELPADRIFEALAEALGKPSPSPPE"
     gene            2114..3367
                     /locus_tag="AC16CM_12790"
     CDS             2114..3367
                     /locus_tag="AC16CM_12790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3140756.1"
                     /translation="MESHPSHSASAGHDGTGGVFFDDPLFEQFAVRSMTLDGCPLGEI
                     STIASSIEEGDADSWFQEWSAAGDRLGGYGDASAHADHAISASGSYLRAASYYRAAYA
                     PLFGSPVDARLVEAFDKEAAAFQKAAALMPSPVEPVEIPFEGTTLPGYFCRVDDSDRP
                     RPTLICTNGYDSTIYEMYLDFAAPALRRGYNLLLFDGPGQGRPLIRQGLKMRPDWENV
                     VGPVVDYAITRQEVDPEKIALMGWSFGGYLAPRAASAEHRLAACIADPGLWDLLESMK
                     ASFTVLPKEVLEKFPDVDPEVLKPLEEHIRAIPALRWAVVQRAFWVHGIDSLAEYVHI
                     SQYYSLKEAAGRIRCPTLLTLAESDPLSWNAERIHESLRCPRELVRFTNAEGAGDHCE
                     GKARPLFHRRAFDWLDETLEVSPER"
     gene            3703..5217
                     /gene="dacB"
                     /locus_tag="AC16CM_12795"
     CDS             3703..5217
                     /gene="dacB"
                     /locus_tag="AC16CM_12795"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3140757.1"
                     /translation="MDRPKYQYGQWGYLEVDPSNGRTVRSLGPADRFYIPGSTTKLFS
                     VSPTLDYLGFDHRFKTPVYAQGEVKNGTLNGNLVLVASGDLTMGGRTAPNGTVSYTHI
                     DHTYANDAPGATLTPENPLAGLDEIAKQVRKSGIERVNGDVMIDDRLFELAPKGPSVN
                     ALSFNAPNLDPWPRPITINDNLIDVVVAPGKVGKAPKVVKWRPKVAPYRIEMRAETVA
                     AGKPTTLTVSPQTDGPVVVSGNIATDAGKQLRVAAVDDPPAFARTALIEALGRSGVSV
                     GVTPTGPNPSSKLPKKGSYSSRDEVAAYVSPPYSEYAELILKVSHNYGANLDLCLMAV
                     KAGSTDCNDAFPVMEKFYEKAGVDTSQVAFADGRGGNPTDRFTPKAATEVLRYWLKQP
                     QAKTFREMLPIMGVDGSLADNCKSCPAKGKVFAKVGTVSLPDYVDGSLLDNESIGGYM
                     EVKPGTFHVFYLVVNGARAKDIEGAIEILNEVNDMAATLQQDAPQQGGASQDGQ"
     gene            5328..6524
                     /locus_tag="AC16CM_12800"
     CDS             5328..6524
                     /locus_tag="AC16CM_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HIR3140758.1"
                     /translation="MSTTRTLNSFFPGALAALVVALVAAMVALLVLAYDASPARAAGD
                     CSTTSGTTTCTFGSTGEEDTFVVPAGVSSVQVVATGAPGAAGDFYPDIGAIPALGGRG
                     AVVSGNLTGLTPGDTLYVNVGGAPTGGDAAGDCADSINCHGGFNGGGNASALGGGGGG
                     GASDVRYVSRDRSGSLASRLIVAAGGGGGGVGGVCMSLLDPDPQDLQPLLGGTGGDTF
                     SDGGDGESCVVEIGTLPYIVGGGKGGKAGTQNAGGAGGAGGTVPGGGSGLDGESGSLG
                     QGGSGAPGGGGAPYGGGGGGGGYYGGGGGGGSAVCPNDVVCSGLWSGAGGGGGGSNLV
                     PPGGSGPRDTALSPYIAISYRAGGVGEPPPTEQPQSKGDCKKGGYEEYSFKNPGRCIK
                     AVNHPR"
     gene            6593..6904
                     /locus_tag="AC16CM_12805"
     CDS             6593..6904
                     /locus_tag="AC16CM_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3140759.1"
                     /translation="MTEASGGSMRSELQRRLVQRSIEDEDFRKKLLDDPKWAVEQVLQ
                     TQLPEDIEVRVVEESPKTIYLVLPGTGLPPDEEGELSDQELEAVAGGGTWSGDSCQCP
                     S"
     gene            complement(7525..7755)
                     /locus_tag="AC16CM_12810"
     CDS             complement(7525..7755)
                     /locus_tag="AC16CM_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140760.1"
                     /translation="MFDLLGEETGHTKRTNFGAIGRYFVDRLQQVFVGVSKNPLPLPN
                     SKGVLLFLLCFAAANPKSAKPALRIANHMLRR"
     gene            7902..8333
                     /locus_tag="AC16CM_12815"
     CDS             7902..8333
                     /locus_tag="AC16CM_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3140761.1"
                     /translation="MMDPFPTVILLATDGSEEARTASRMAVGIAQGTGSELHLVHVGG
                     MGGADPRLYEQMRRNAQSLLEEQAGELREAGAKIAKRHLKNGRPEQEIVALAEELGAG
                     LIVMGSRGFGGMKRTLMGSVSDSVVRHAHCPVLVARGSRPR"
     gene            complement(8368..9672)
                     /locus_tag="AC16CM_12820"
     CDS             complement(8368..9672)
                     /locus_tag="AC16CM_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008414506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3140762.1"
                     /translation="MVVLALFLVALNGLFVAAEFGLVKIRSTQVDRLVREGKTSAGLV
                     KEATNRLDAYLSVCQLGITISSLGLGWIGEPAFAHLLEPLLEPLGVPEGSIHVIAFAA
                     AFATITFLHVVFGELAPKSVAIARAEGTSLFVAPFMRFFYYLFYPGIVVFNGAANAVV
                     RLFGVPPASGTEETHSEEELRILIEQSTRQGVLNIGEEHMLEAVFELEDTRAREIMVP
                     RPDVVALSANTRLDELVVATAGNHTRYPVFEGDSQERIVGMVHVKDVLETIKQTRSLE
                     ADVSARDLMRDVLIVPENRPVDELLKDLQKRGIQMAVVIDEWGSFEGIVTMEDIVEEI
                     VGEIRDEFDEEEPHVERLEDGSYAVDGRIPIGTVNEVVGSDFQSEDFDTIGGLVLGRL
                     GRIPEVGDEVSMGEHVLRVEEVDGPRVARVKVKNNPAQRPGT"
BASE COUNT         1983 a         3218 c         3357 g         1756 t
ORIGIN      
        1 ggtgggggcg ttaatcaact ctaggcgggg ctttactcac cccaccgctc tttacgaaag
       61 gtgattccag cacgaatgag tactactaga acactcaact cctccttctt cctggggccg
      121 tggccgcttt ggtggtggcg atggtgagtc gtgcctccta gacctcggga cctttaccta
      181 taccctcgag ggaggcacgg gcggtaaggc gggcacccag agcgccggtg gcgcaggggg
      241 cgtccccaac ggcgccagcg ggtcgctggg gcagggcggc cacagcggac ccctcggcgg
      301 tggcggcggc tccaacctgg tcccacaggg cggctccgcg accctggccc aggacacctc
      361 cgtccctccg tcggtgacca tcagctacag gaccggtctc gtgggcgagc ctcctcctac
      421 cgagcagcca cagagcaaag cggattgcag gaagggtggc tacgaggagt acggcttcaa
      481 gaaccaggga ttgtgcatca agggcgtaaa ccacccacgc tgaggaaccc tacgactaat
      541 actctgtagc cgttggtagg gggccggggc acatctctct aagccccgat cctcaaagat
      601 agctcatttg ggggatacgc ccttactctg cgggtctata cagtacttgc atgcgcgtac
      661 tcggtgtcgc gctaaaggcg aattcgccgc gaggtggggg cgcgaagacc atagggcctc
      721 tccccgggag ggaggtggcc gggctggctg gttgatggcc gactgggagg agaagcgttt
      781 gttcgttcgg aatatgcacc tgtcgatgtg gacggcggcg tttctcgtca tcgggtcggt
      841 gctcctgctt gggagcggat gcggtggggg tgatggtggc gaagggggtt cctccacggg
      901 tacccaaaac aacaagggtg gcgctcaggc tacagaggac gatctgaacg cggcgctcga
      961 gaagtcattt aaagagaccg acgcgcccgg ggtggtggcc gccgtggaga ccccggacta
     1021 cacgtgggtg caggccaagg gggtggccga ccgcgctagt ggggagcaga tgacgccgga
     1081 ggtgcaccac cggataggca gcgtcaccaa gaccttcacc gccaccctcc tgctcaaggc
     1141 cgcagacgac gggctcctct cgctcgacga caccatcgac cagtacgtca agggcgtgcc
     1201 caacggggac gagatcacgc tcaggcaggt ggccgatatg accagcggta tcgccagcta
     1261 cacggaatcg gaccagtggg tagaggagtg gtcctccaac ccgaacaagg tctggaagcc
     1321 cgaggatctg gcacagtatg gcatcgagga gtcccccctg ttcaaatcgg gtacggcgtg
     1381 gttctactcc aacaccaact acgtgttgct tggtctggta ctccagcagg tgacgggcaa
     1441 gcccatcgag cagctctacg aggaagagat cataaggccg ctggacctca ccgagacgtc
     1501 cttcccgggc aagagctccg cgataccgga gccttacgac cacggctaca ccctgcaggg
     1561 aaagtcctcc ggccagaagc ccatagactc cacccactgg agcccatcca tggcctggac
     1621 ggcgggcgag atgatctcga cggtagatga cctgctggtc tacggccggg cgcttggaac
     1681 cgggaaggga cttctctcac ccgagatgca gtccgagcgg ctcgactctt tcgtcagcga
     1741 cgtgccgccc ctgaaccagc ccccgctcaa aggcgacctg gagtacggca tcgggctagg
     1801 gaaggaccac gggtgggtcg gacacaacgg ggagataccc ggctacaaca cctacctctt
     1861 ctaccatccg gacctcgacg ccgtggtggt ggtgctggtc aacagcgaca tctcctccgg
     1921 caagtgcccg aaggacgtgc cgatcatgaa ggcatggccc aggaacgcct cctgcgagtt
     1981 accggcggac cggatcttcg aagccctggc cgaggcgctg ggcaagccct caccatcgcc
     2041 gcccgagtag ccaacccgaa tgccaattgg ccggccccat aggccggcca cgcatccagg
     2101 aagcgaggaa tagatggagt cacacccgag tcacagcgca tccgccgggc acgatggaac
     2161 cggaggcgtc ttcttcgacg atccgctgtt cgagcagttc gccgtgcggt ccatgaccct
     2221 ggatggctgt ccgctaggcg agatctccac gatcgcatcg tcgatcgagg agggagacgc
     2281 cgacagctgg ttccaggagt ggagtgccgc cggcgatcgt ctgggcggtt acggcgatgc
     2341 cagcgcccac gcggaccacg ctatcagcgc cagcggttcc tatctccggg cggccagcta
     2401 ttaccgcgcc gcctacgcgc cgctctttgg ttcgccggta gatgccaggc tggtggaggc
     2461 gttcgacaag gaggccgcgg ctttccagaa ggccgcggcc ctaatgccct cgccggtcga
     2521 gccggtggag attcccttcg agggaaccac gcttcccgga tacttctgcc gggtcgacga
     2581 ctccgaccgg ccccgtccga cccttatctg caccaacggc tacgactcga cgatctacga
     2641 gatgtacctg gacttcgccg cgcccgcgct tcgccggggc tacaacctgc tgctcttcga
     2701 cggtccgggg cagggccgtc cgctgatccg gcagggcctc aagatgcgcc ccgactggga
     2761 gaacgtcgtc gggccggtcg tggactacgc catcacgcgc caggaggtcg accccgagaa
     2821 gatcgcgctg atgggctgga gtttcggcgg ctacctcgcg ccccgagcgg cgagcgcgga
     2881 gcaccgcctg gccgcgtgca tcgcggaccc cggcctgtgg gacctgctcg aatcgatgaa
     2941 ggcgagcttc acggtcctcc cgaaagaggt gctggagaaa ttccccgacg tcgaccctga
     3001 ggtgttgaag ccgctcgagg agcacatccg ggcgatcccc gcgcttcgat gggccgtcgt
     3061 ccagcgagcc ttctgggtgc acgggataga ttccctcgcg gagtacgtgc acatctccca
     3121 gtactacagc ctcaaggagg cagcggggcg cattcgctgc ccgacactcc taaccctggc
     3181 agagagcgac ccgctctcgt ggaacgcaga gcggatacac gaatcgctga gatgccccag
     3241 agagttggtc cgattcacga acgccgaagg ggcgggcgat cactgcgagg ggaaggcccg
     3301 cccgctcttt caccggcgcg ccttcgactg gctcgatgaa accctggagg taagccccga
     3361 gagataggct tcctgaaatc atgctcaccg gagtgcgtgg aagatgattt ctgcgaactt
     3421 cgctttctag agaggagtct cactatgtcc accgccgctc ccgtacagcc cgctgctccc
     3481 cgcaggagcc tattgaggaa gacgatccta ctgattgcgt cgatggccgt ggcggttagc
     3541 atggcgttgc tccttggctg cgaggccgac acacaagggg gatctcaagg ggagtctggc
     3601 tcgtccaaga ccacgtcgaa tgcccagacg accgaagtga cggggcagac ccagggcagc
     3661 gaccgagcgc gatcggagct aggtccgaag gtagagaaga tcatggaccg cccaaaatac
     3721 cagtacggcc agtggggata cctcgaggtt gatccctcca acggtcgcac cgtgcgctcg
     3781 ctgggccccg ccgaccgctt ttacatcccc ggctctacga ccaagctctt cagcgtctcg
     3841 ccgaccctcg actacctcgg cttcgaccac cgtttcaaga cgccggtcta tgcgcaggga
     3901 gaggtcaaga acggcacgct caatggcaat ctggtgctcg tggccagcgg cgacctgacc
     3961 atgggcgggc gaaccgcacc caacgggacc gtctcctaca cccacatcga ccacacttac
     4021 gccaacgacg cgccaggggc tacgctcacc cccgagaatc cgctcgcagg cctcgacgag
     4081 atcgccaagc aggtgcgcaa gtccggcatc gagcgtgtga acggcgacgt catgatcgac
     4141 gaccggctct tcgagctcgc ccccaagggc ccgtccgtga acgccctctc cttcaacgcc
     4201 cccaacctcg atccctggcc gaggccgatc accataaacg acaacctcat cgacgtggta
     4261 gtcgcccctg gcaaggtcgg gaaggcaccc aaggtggtca agtggcgacc caaggtagct
     4321 ccgtaccgca tcgagatgcg ggcagagacg gttgcggccg gcaagccgac caccctgacc
     4381 gtgagccccc agacggatgg tccggtggtg gtgagcggga acatcgcgac cgacgccggc
     4441 aagcagctga gggtcgcggc ggtagacgac ccgcccgcct tcgcgcgcac ggccttgatc
     4501 gaggccctgg ggcgctccgg cgtctccgtg ggcgtcaccc ccacaggccc taacccttct
     4561 tccaagctgc cgaagaaggg ctcgtactcg tccagggacg aggtcgcggc ctacgtctct
     4621 ccaccctaca gcgagtacgc cgagctcatc ctcaaggtca gccacaacta cggcgccaac
     4681 cttgacctgt gcctgatggc cgtcaaggcc ggaagcaccg actgcaacga tgccttcccg
     4741 gtgatggaga agttctacga gaaggctggc gtcgacacgt ctcaggtggc tttcgcggac
     4801 ggcaggggcg gcaaccctac ggaccggttc acccccaagg cggcgaccga ggtgcttcgc
     4861 tactggctca agcaacccca ggcaaagacg tttcgcgaga tgctacccat aatgggcgtc
     4921 gacggtagcc tcgcggataa ctgcaagagt tgcccggcaa agggcaaggt cttcgccaag
     4981 gtcgggacgg tcagcctgcc agattacgta gacgggagcc tgctcgacaa cgagtcgata
     5041 gggggttaca tggaggtcaa accaggtacc ttccacgtct tctacctggt tgtcaacggc
     5101 gcgagggcga aagacataga gggcgccatc gagatcctca acgaggtcaa cgacatggcc
     5161 gccacactgc agcaagatgc cccgcagcaa ggaggtgctt cacaggacgg ccaataacgg
     5221 cggctagcaa caacataccc tgacggtgac acggtgatgg cgttgttcaa ctgtaggcgg
     5281 ggctttactc accccgccgc tctttacgaa aggtggtatc ggcacgaatg agtactacta
     5341 gaacactaaa ctccttcttc ccgggagccc tggccgcgtt ggtggtggcg ttggtggcgg
     5401 cgatggtggc gctccttgtg ctcgcctacg acgcgagccc cgcccgggca gcgggtgatt
     5461 gttcgaccac ctccggcacc accacctgca cctttggctc gacaggtgaa gaggacacct
     5521 tcgtggtgcc agccggggtg agcagcgtac aggtcgttgc caccggagct ccaggcgccg
     5581 cgggggactt ttatcctgat attggcgcta tccctgcgct cggcggtcgt ggtgcagtgg
     5641 tgagcggcaa cctgacggga ctcacgcccg gcgataccct ctacgtcaac gtcggcggcg
     5701 caccgacagg aggggacgca gcaggcgact gcgctgatag cattaactgc cacggcgggt
     5761 tcaacggtgg cggaaacgca agcgccttgg gtggtggtgg cggcggcggt gcctcagacg
     5821 tgcgctacgt atccagggac cggagcggaa gcctggcctc gcggctgatc gtggccgcag
     5881 gaggcggagg cggaggagtg ggtggtgtgt gcatgtcgtt gttggaccct gaccctcagg
     5941 acctccaacc actcttgggg ggtaccggcg gtgacacctt ttcggacggc ggcgatggtg
     6001 agtcgtgcgt cgtggagatc gggaccttac cctatatcgt cggcggaggc aaaggcggta
     6061 aggcgggcac ccagaacgcg ggcggcgcag ggggcgccgg gggcaccgtt cctggcggcg
     6121 gcagcggctt ggatggcgag agcgggtcgc tggggcaggg tggcagcggc gcccccggcg
     6181 gtggcggcgc accctacggc ggtggcggag gaggcggcgg ctactacggc ggaggcggcg
     6241 gcggaggcag cgcagtttgc ccgaatgatg ttgtttgctc agggctttgg tcgggcgcgg
     6301 gtggaggggg cggcggctcc aacctggtgc caccaggcgg cagcggaccc cgcgacaccg
     6361 cactcagccc gtacatcgcc atcagctaca gggccggtgg tgtaggcgag cctcctccta
     6421 ccgagcagcc acagagcaaa ggggattgca agaagggggg ctacgaggag tacagcttca
     6481 agaacccggg gcggtgcatc aaggccgtaa accacccacg ctgaaaaaca ccacgacgac
     6541 tacaacttcc cgttgcgaac tgtatgcagc gagagcgaaa ggggattcga tcatgaccga
     6601 agcgagcggc ggcagcatgc gctcagagtt gcagcgcagg ctcgtacaga ggagcatcga
     6661 ggacgaggac ttcaggaaga agctactcga tgaccccaag tgggccgtgg agcaggtgct
     6721 tcagacgcag ctgcccgagg acatcgaagt aagggtggta gaggagagcc cgaagaccat
     6781 ctacctggtg cttcccggca ccggcttgcc gcccgacgaa gagggggagc tctccgacca
     6841 ggagctagag gcagtagccg gtgggggcac gtggtcggga gactcgtgcc aatgcccgag
     6901 ttgagacgtt gctgagcgtt caggcatagc tccgagaatt agccttccag gcggttgggt
     6961 gaacaagggc ttgcagaagg aggcccggag cccttagctg cctcggtccc ccactaacgg
     7021 ctacagttat ggggtagcgc ggccttcacg ggcaacccat ctcgcaagac acggggcttg
     7081 ttcacctgcg gcgctctgcg aaatagagga actggtgttg agagggaggt agttatcgga
     7141 gtctggtgac gagagcgtac cctatctctt tccctgccaa ttggctagaa acgagctcga
     7201 acggcgttct cgaggaggtt caccatcgga ggcaaagata gctcaattag gggatacccc
     7261 tggtgccgat ggttgctagc gtgtttgagt tgttcaactc taggcggggc tttactcacc
     7321 ccgccgctct ttgcggaagg tggttccagc acgaatgagt actactagaa agaacagctc
     7381 cttcatcctg gggccctatg gcctgctcgg agcagaatcc ggcttacggg acggcccgga
     7441 ccaacgattt gcaggtaaga ctcggaatca taacccttgt ggatacggat ttgaccccac
     7501 gttttacccc taccagctcg ctaatcacct ccgcagcatg tggtttgcga ttctcaatgc
     7561 gggcttagcg cttttcggat ttgctgccgc aaagcacagc aagaacagca gcacgccctt
     7621 cgaattcggg aggggcaacg ggttcttgga gaccccgacg aatacctgtt ggaggcgatc
     7681 cacgaagtag cgtcctatgg ctccgaagtt ggtacgcttg gtgtggcctg tctcctcgcc
     7741 caagagatcg aacatgctag gctgattgct ggcctcctcg tagaacgctt ccttccaacc
     7801 gtcggtctaa tgagcctacc aactcctggg agtggtcccg cgtgcgagcc tcctcaggcc
     7861 attagaatgt agggacgcgc gttaggcgga gagagcgagt gatgatggac cccttcccca
     7921 cggtcatact cttggctacc gacggctccg aggaggcccg tacggcatct cggatggcgg
     7981 tgggcatagc gcagggcacg ggctccgagc tgcacctcgt gcatgttggc ggtatgggag
     8041 gggccgatcc gcgcctctac gagcagatga ggcgaaatgc gcagtccctt ctcgaggagc
     8101 aagccggaga gctgcgcgag gcgggggcga agatcgcgaa aaggcacctg aaaaacgggc
     8161 gcccggagca agagatcgtg gcgttggccg aggagcttgg agccggacta atagtgatgg
     8221 gcagcagggg cttcggcggg atgaagcgga cgcttatggg tagcgtctcc gactcggtcg
     8281 tacgccacgc ccactgcccg gtgctggtgg cgcgcgggag ccgaccacgc tgatctggga
     8341 gctatggagc cgacttcgtc gatgccttca cgtgcctggt ctctgggccg gattgttctt
     8401 cactttcacc cgagccacgc ggggaccgtc cacttcctcc acccgcagga cgtgctcacc
     8461 catgctcacc tcgtcgccga cctcggggat gcgtcctagc cggccgagga ccaagccgcc
     8521 tatggtgtcg aagtcctcgc tctggaagtc agagcccacg acctcgttga ccgtcccgat
     8581 agggatgcgt ccgtccaccg cgtaggatcc gtcctcgagc ctctccacgt ggggctcctc
     8641 ttcgtcgaac tcgtcgcgga tctcgcccac tatctcctcg actatgtcct ccatcgtcac
     8701 gatgccctcg aaggagcccc actcgtctat gaccaccgcc atctggatcc cccgcttctg
     8761 gaggtctttg agcagttcgt ctacggggcg gttttcgggg actatgagca cgtcgcgcat
     8821 gagatccctg gcgctcacgt cggcctcgag gctcctcgtc tgcttgatcg tctccagcac
     8881 gtccttgacg tgaaccatac cgactatgcg ctcctgcgaa tcgccctcga agacggggta
     8941 gcgggtgtgg ttgcccgccg tggccacgac cagctcgtct agtctcgtgt tggccgagag
     9001 cgcgaccacg tcggggcggg ggaccatgat ctcgcgcgcc cgcgtgtcct ccagctcgaa
     9061 gaccgcctcc agcatgtgct cctcgccgat gttcagtacg ccctggcgtg tcgactgctc
     9121 tattaagatg cgcagctcct cctccgagtg ggtctcctcc gtccccgagg cgggcggtac
     9181 gccaaagagg cgcaccacgg cgttggccgc cccgttgaag acgactatgc cggggtagaa
     9241 gaggtagtag aagaacctca tgaaaggcgc cacgaaaagc gaggtgccct ccgccctggc
     9301 tatggccacg ctcttgggcg cgagctcccc gaagaccacg tgcaagaagg ttatcgtggc
     9361 gaaggccgcg gcgaaggcga tcacgtggat gctaccttct ggtacgccca gcggctcgag
     9421 cagcggttcg agcaagtggg cgaaggccgg ctctcctatc cacccgaggc caagggagga
     9481 gatcgttatg cccagctggc ataccgagag ataggcgtca agcctgttcg tggcctcctt
     9541 gaccagcccc gcggaggtct tgccttcccg cacgagcctg tccacctgcg tggagcggat
     9601 cttcacgagc ccgaactccg ccgccacgaa caggccgttg agcgcgacca gaaacagcgc
     9661 gaggacgacc atgagggcgt cgaggacaaa gccgctcaat acggcgcctc caccagcgga
     9721 gaccccttag ggggagccgc ctcgcgtccg tctggccgtc ccgagaggga gaccacaggc
     9781 gcgggagcgg gcaacagttc gactacgttc atgcagtgca cctagcgtac cacgccgacc
     9841 cggccaacga cccgttacgc cgctctccta cccggagtgc ctgcgacgct acgcaaggca
     9901 ctcgtgccgg gcgaagcttc ggggccttag gtcgagctag gccccgagat cagccggtag
     9961 ggtaagtttc cgagagctcc gtctacgaaa ctgtttggaa agtcgccgag ggcctctcga
    10021 gctggctccg tcggccccgc gtgctacaca gcgcggtcaa ttgtggtgga ttgtagggat
    10081 tggcacatcc gccggacggc acatctttac cgttactcgg acgctgtgag cggatggtca
    10141 acatcttagg acgccgtttg ggcgaagttc ggagaatgcc ttctaggcga agttcgcaga
    10201 aaaacccttc cacgcactcc gatgaataag ggcacgaaga ggagagggag gaccagttcg
    10261 gggacgtaca cgggaatttc cagcgccgga gggcccgtcg cgggccaccg gact
//
LOCUS       DBNGVD010000477         3691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2303032, whole genome shotgun sequence.
ACCESSION   DBNGVD010000477 DBNGVD010000000
VERSION     DBNGVD010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2303032"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1175
                     /locus_tag="AC16CM_12825"
     CDS             282..1175
                     /locus_tag="AC16CM_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3140763.1"
                     /translation="MHPTKVTEKYFERGIQGVGGRAMLLLAAIFAALTLSSGVALAAT
                     ISCQADKECYGTRMADTLAGTDREDHIFGRGGSDVIKGQEDLDYLYGQGGSDRLIGGP
                     DDDLLTGGSGDDILRGGTSPASYIFDDGWGKDTLVDPEPDPAYEVDTLDFRRYSTLFV
                     AEDLTIKLSSGEGPEVTNLDGTDTIDWEGNPIEQVRSGDGDDDITGDSLANILVAHGG
                     DDTINGSGGQDTISGGTGEDNIAGEQGNDTIYANDGSGGDTVDCGEDFLIDHDKVYYD
                     LLDQVSSNCEEKHLAAQLPNP"
     gene            1366..>3691
                     /locus_tag="AC16CM_12830"
     CDS             1366..>3691
                     /locus_tag="AC16CM_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140764.1"
                     /translation="MLPSDVGEGPDPRLVRDVQRSGSHASAESEAVPGVEARYRPPGD
                     LPVELSSLVGREREISAVKNLLHDHRLLTLVGPGGQGKTRLALAAASGLSEDFEDGVW
                     WVGLASLSDPDLVPQVVAQALQVREQPGIPLTEVLVRSLLEERLLLVLDNCEHLAGAC
                     ARFARTMLSSCPGVRILATSREVLGADGEMNWRVPPLPVPNESGLSSVEEMGGHAAVA
                     LFVERARARLPGFTLTGENALAVAEVCRKLDGLPLAIELAAARVPMLSLGEISERLEK
                     PLKFLGGTSRRGEARHRTLRATLDWSHEMLSQEERSLFRRLSLFAGGFDLEAAESVGA
                     GDGIEPEMVLDPLSGLVDKSLVVAEDGGEVLRYRMLEPVRQYALEHLEESGEATTARD
                     RHASLFMALAERSHPEMRGPGQVGWILRLGRENDNLRGAMSWALSSGDSVTAARLGSA
                     LWPFWWYRGQHREGRNLMEAALEGELEPPLRIRATVAAAIMTYGQADNDGVMRYMDTL
                     QGLSRQAGGDAYADGYAHAGLGLVAMNLGDFDEARAQLQEALPLLVESGEVWTASQAH
                     TWIGTVALLEGDNERAMTRFEEGMALAREIGDRTGMYNALYALASMSLAGGDYPAAKG
                     RFEEAMALSEEMGDRANAAYCLEGLAAVAASQEHPERAARLFGAAEKLLESIGVPVWT
                     SYKPDRAFYEQTIENVRSGLGPDAFEDARQMGRAMTPEHAIAYALEEERARPSLPAGL
                     SEREAEVLALAAEGLTNAQIAQRLYISPRTVNRHM"
BASE COUNT          698 a         1121 c         1281 g          591 t
ORIGIN      
        1 aggggtgcct cgcaacctgg gatgggcagc ggttccacac cacgaaaccc gtgatgaacg
       61 gggtttagat ccgctccggg tacgcctgcc cccgtaccag tccttcttag ccctcgacga
      121 gagacaggtt cgtttcggga taggggtacc cgtgcccatc tttccaggaa tgggtacccc
      181 agatgggtag ttctgacgat gtcgccttgt ttgccgggac gcaatatgta cccgagtaaa
      241 cgacgcctgc cagagaagga gcgggggaga ggagtaggac gatgcacccg acaaaggtca
      301 cggaaaagta ctttgaaaga gggatacagg gggtaggcgg tcgagcgatg ctgcttctcg
      361 cggcgatctt cgcggccctg acgctttcca gcggggtagc actggcggcg acgataagtt
      421 gccaggccga taaagagtgc tacggtacca ggatggccga taccctggca ggcacggatc
      481 gggaagatca tatcttcgga cgggggggta gcgacgttat caagggccag gaggacctcg
      541 attatctgta cggccagggc ggtagcgaca ggctcatcgg tggtcccgac gatgatctgt
      601 tgacgggagg ttccggcgac gacattttga ggggcggcac cagccccgcc tcctacatct
      661 tcgacgatgg ttggggcaag gacaccctgg tagacccgga gccggatcct gcctacgagg
      721 tggacacgtt ggacttcaga aggtactcga ccctattcgt tgccgaggac ctgaccatca
      781 agctgtcatc gggcgagggg cccgaggtga ccaacctcga cgggacggac accatagact
      841 gggaaggcaa tcccatcgag caggtccgaa gcggcgatgg tgacgacgac atcacaggcg
      901 acagcctggc caatattctt gttgcccatg ggggcgacga caccatcaac ggatcggggg
      961 gacaggacac catctccgga ggaacgggcg aagataacat agccggagag caaggaaacg
     1021 acaccatcta cgcgaacgac gggtccggcg gcgacaccgt ggattgcggc gaggacttcc
     1081 tgatcgacca cgacaaagtc tattacgacc tgctcgatca ggtatccagc aactgcgagg
     1141 agaagcacct cgccgcgcag cttcccaacc cgtaagcact ggatccgttc tacagatgga
     1201 cggggctcgc tgtctagcga gccccgtcct ctcgctcgtc cggggcgcga agagatcgtt
     1261 acgcgtcaca tcatccacat caacccgtga gggatgcgcc ggaataccac ttgagctacg
     1321 ctaataccgc aggtcgcacc acacgcatac ggggagtacg tggcgatgtt accttcggac
     1381 gtgggagaag gccccgaccc acgcttggtt cgggatgtgc agaggagcgg gtctcacgcg
     1441 agcgcggaaa gtgaggcggt tccgggtgtt gaggctcggt accgtccacc cggagacctg
     1501 ccggtagagc tgagtagtct ggtcggtcgc gagcgcgaga tctcggctgt caagaacctg
     1561 ctgcacgacc accggctgct cacgctcgtc ggccccggag gccagggcaa gacgcgtctt
     1621 gccctggccg cagcctcggg gttgtcggaa gatttcgagg acggcgtgtg gtgggtgggg
     1681 ttggcctcgc tgtcggaccc cgacctcgtc ccacaggtag tcgcccaggc cttgcaggtg
     1741 cgtgagcagc caggcatacc gctgaccgag gtgctcgtcc gatccttgct cgaggaacgg
     1801 ttgcttctgg ttctggacaa ctgcgagcac ctcgccgggg cctgcgcccg cttcgcgcgg
     1861 acgatgctat cgtcctgtcc cggggtacgc atcctcgcca ccagcaggga ggttctcggc
     1921 gcagacggag agatgaactg gcgggtgccg cccctcccgg tgccgaacga gagtggcctg
     1981 tcgtcggtag aagagatggg gggtcacgcg gcggtagcgc tcttcgtgga gcgtgcgcgg
     2041 gccaggcttc ccggtttcac cctcacgggg gagaatgctc ttgcggtcgc cgaggtgtgc
     2101 cgcaagctgg acggtctgcc cctggcgata gagctcgcgg cggcgagggt gcccatgctc
     2161 tccctgggcg agatctccga acggctggag aaacccctca agttcttggg cggcacctcc
     2221 cggaggggag aggccaggca caggacgctc agggccaccc tggactggag ccacgagatg
     2281 ctctcgcagg aggagcggag cctgttcaga cggctctccc tgttcgccgg cgggttcgac
     2341 ctggaggcag ccgagagcgt cggcgccggg gacggcatag aacccgaaat ggttctcgat
     2401 ccactctcgg ggcttgtgga caagtcgctc gtggtcgccg aggatggcgg agaggtcttg
     2461 cgctacagga tgctcgaacc tgtcaggcag tacgcgctgg aacacctcga agagagcggg
     2521 gaagccacga ccgcgaggga ccggcacgcc agcctgttca tggcgctggc cgaacggtcg
     2581 catccggaga tgcgtggtcc ggggcaggta gggtggatcc tgaggctcgg cagggagaac
     2641 gacaacctgc ggggcgccat gtcgtgggcc ctctcctccg gggattctgt caccgccgcg
     2701 cggctcggct ccgccctgtg gccgttctgg tggtacaggg gccagcaccg tgaaggacgg
     2761 aacctcatgg aagctgccct cgaaggagag ctggaaccgc cgctccgcat ccgggccacg
     2821 gtggcggcgg cgataatgac ctacgggcaa gcagataacg acggggtcat gcgctacatg
     2881 gacacgctcc aggggctgtc ccgtcaagcg gggggcgacg cctacgccga tggctacgcc
     2941 cacgccgggc tcgggttggt ggccatgaac ctcggcgact tcgatgaggc cagggcccag
     3001 ctccaggagg cactgcccct actcgtcgag tcgggcgagg tgtggaccgc ctctcaggcg
     3061 cacacctgga tcggcacggt cgcgctcctc gaaggcgaca acgagcgggc gatgaccagg
     3121 ttcgaggagg ggatggcgct cgccagggag ataggagaca ggacaggcat gtacaacgcc
     3181 ctctacgccc tcgcctccat gtcgctggcg ggcggagact atcccgccgc gaagggtagg
     3241 ttcgaggagg ccatggccct ttccgaagag atgggcgatc gggcgaacgc ggcctactgc
     3301 ctggaggggt tggccgccgt cgccgcgtcc caggaacacc cggagcgggc cgcgcggttg
     3361 ttcggcgccg cggaaaaact ccttgagtcg atcggcgtgc ccgtctggac ctcctacaag
     3421 cctgaccggg ccttctacga gcaaaccata gagaacgtgc gctccggcct gggccccgac
     3481 gcattcgagg acgccaggca gatgggccgc gcgatgacgc cggagcacgc catcgcctac
     3541 gccctcgaag aagaacgggc ccggccgtcc ctcccggcag ggctgagcga acgagaggcc
     3601 gaggtgctcg cgctggcggc cgaggggctc accaacgccc agatagcgca gaggctctat
     3661 atcagccccc gcacggtcaa ccgccatatg g
//
LOCUS       DBNGVD010000478         3377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2303100, whole genome shotgun sequence.
ACCESSION   DBNGVD010000478 DBNGVD010000000
VERSION     DBNGVD010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2303100"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16CM_12835"
     CDS             complement(<1..413)
                     /locus_tag="AC16CM_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3140765.1"
                     /translation="MDVMRGLGEQGDSTSLAGVDNLMLHFTPYAEDWSKLPVIVSGNG
                     SYVTDDKGNTYVDGLAGLFTTQVGHGRTELADAAHKQMKELGFFPNWSFQHPRSLELA
                     AKIAEIAPGDLNSTFFVSSGSEAVETVIKLARQYH"
     gene            612..2966
                     /locus_tag="AC16CM_12840"
     CDS             612..2966
                     /locus_tag="AC16CM_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3140766.1"
                     /translation="MSRARTENTLRVVAGGSGGKLETVSGEELPLETTSFVGRGRELS
                     EVEGLLGRTRLLTLVGVGGSGKTRLALRVAMRRADEGDVWWVELASLSDSELVPKKVA
                     STLGVAESPGRQTGELLVDYLGSREALLILDNCEHLVGACATLADVLLRACPRVRILA
                     TSREPLGVAGEVSWTVPPLSLPEVGRARIPKELLGCEAVGLFVERVGSVEPGFALTEE
                     NATAVVDLCARLDGLPLAIELAASRVKVLSVGEILERLDDRFRLLRGNRTAVPRHRTL
                     EATIDWSHGLLSEQERTLFRRLSVFAGGWTLSAVEDICSGDGIEKEEVLELLASLVDK
                     SLVVVVRNEQESRYRMLETVRQYASVKLGEAAEREAVSDRHANFLLDLAERAETRLAG
                     PEQAAWLDRLEAEHDNLRVALVWLTERGEAERGLMLAAALLRFWWFRGHLAEGRMRLE
                     GLLDLRPTVPVRDEVRAKALHTLAIHRFSDDTLEDWTMVRSRLQESLKIYRRLGDEPH
                     AAAVLRNLGRVRAVLGDWTAAYASLNESLEIMRRSGTEPDVALSLFYLGMVQLHREDL
                     SPARANLEDGLELFRKLEDKFWVNACLVHLAYIDCEEGAYGAARSRLLQTNDTLPLVQ
                     FPWGATYVLDGFSRLAVAQGESVRALRLGGATDALRQTYGVTIGPTEQAAFRRRLEPA
                     RRVLGEEAGDRAWEEGRAMTLEEAVDLALTETGPGRRPNGLLSDRELEVLRLVASGFS
                     DVEVAENLYLSPRTVGGHLGGIYRKLAVKSRTAAVKKAGELGLI"
     gene            3140..>3377
                     /locus_tag="AC16CM_12845"
     CDS             3140..>3377
                     /locus_tag="AC16CM_12845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3140767.1"
                     /translation="MTGKTHDVAVVGASIAGCTAAILLAREGARVALIERHSDPNAYK
                     ALCTHFIQPSAVPTMERLGLLPLIEAAGAVPNEID"
BASE COUNT          603 a          990 c         1177 g          607 t
ORIGIN      
        1 ttgtggtact gcctggccaa cttgatgacg gtctccaccg cctcagaacc ggaggagacg
       61 aagaaggtgg agttgaggtc cccaggtgcg atctcggcga tcttggccgc aagctccagg
      121 cttctcgggt gctggaagct ccagtttggg aagaagccta gctccttcat ctgcttgtgg
      181 gccgcatccg caagctccgt cctgccgtgg ccaacctgcg tggtgaagag ccccgcgagg
      241 ccgtcaacgt aggtgttgcc cttgtcgtcg gttacgtaag agccattccc agagacgatg
      301 accggtagct tcgaccaatc ctcagcgtaa ggcgtgaagt ggagcatcag gttatccacg
      361 cccgcgagcg aggtggaatc gccctgttct cctagtcctc tcatgacgtc catttgttac
      421 ctccgtaaga cagagccaca cggttccccg cactttgcac gaatgctaca tggttactgt
      481 aaagcggtca agatcatggg ccccaggagc ctcaattgtg gcgcgcccat atgccgcaaa
      541 gatcacccat ttgtaccatg cggtacgcga agtgtggtgt taccctctct ctgggagata
      601 tagtaacaat aatgtctaga gcgcggacgg aaaacacgct tcgggttgtc gccgggggat
      661 cgggcggcaa gctggagacc gtgtcaggag aagagcttcc gctggagacg accagcttcg
      721 tggggcgcgg gcgggagctg tcggaggtgg aaggtctact cggccggacc cggcttctca
      781 cgctcgtcgg ggtggggggc tccggcaaga cgcgcctggc cctgcgggtc gcgatgcggc
      841 gggctgatga gggcgatgtg tggtgggtgg agttggcctc cctttccgat tccgaactcg
      901 tccccaaaaa ggtcgcttcg accctgggcg tggccgagtc gccgggacgc cagacgggcg
      961 agctactagt cgattatttg ggatctcggg aagcgctcct gatcctggac aactgcgagc
     1021 atctggtggg ggcatgcgcg actctcgcgg acgttttgct gcgcgcctgc ccccgcgtgc
     1081 gcatcctggc gacgagccgc gagccgcttg gcgtggccgg ggaggtatcg tggacagttc
     1141 ccccgctctc gctgcccgag gtggggcgtg cacggatccc gaaagagtta ctggggtgcg
     1201 aggcggtcgg gctcttcgtc gagcgggttg ggtccgtcga gcctggtttc gccctgaccg
     1261 aagagaatgc cacggcggtg gtggatctgt gtgcgaggct cgacgggttg ccgctggcca
     1321 tagagctcgc cgcctccagg gtcaaggtac tctccgtggg ggagatcctg gaaagactag
     1381 acgaccgttt ccgcctcttg cggggtaaca ggaccgccgt tccccgccac aggacgctgg
     1441 aggcaaccat agactggagc catggtctcc tttccgagca ggagaggacc ctgttcagga
     1501 ggctctcggt tttcgccggc ggttggacgc tttcggcggt ggaggacatc tgctccggcg
     1561 acgggatcga aaaggaagag gtcctcgagc tgctcgccag cctggtggac aagtcgctgg
     1621 tggtcgtggt gcggaacgag caggagtcac gttaccggat gctggagacc gtcaggcagt
     1681 acgcgtcggt aaagctagga gaagcggcag aacgggaggc cgttagtgac aggcacgcga
     1741 acttcttgct cgatctcgcc gagcgggccg agacgaggct ggcgggcccg gagcaggcgg
     1801 cgtggttgga tcggctggag gccgagcacg acaacctgag ggttgcgctt gtatggctga
     1861 cggaacgagg agaggcggag cggggcttga tgctcgcggc ggcgctgctc cgattctggt
     1921 ggtttcgggg gcatcttgcc gaaggacgga tgcggctcga ggggctgctc gacctacggc
     1981 ccacggtgcc ggttcgggac gaggtgcggg ccaaggcact ccatacgctc gcgatacacc
     2041 gtttctccga cgacaccctc gaagattgga cgatggtacg ctctcgcttg caagagagcc
     2101 tgaagatata caggaggctg ggagacgagc cacacgcggc cgccgtcctc cgaaacctcg
     2161 ggcgcgtcag ggccgtgctc ggtgattgga cggcggcgta cgcttccttg aacgagagcc
     2221 tggagatcat gcgccggtcg ggcaccgaac ctgacgtcgc cctctcgctc ttctatctgg
     2281 gcatggtgca gctacatcgc gaagacctct cgccggcacg cgccaacctc gaggacggcc
     2341 tggagctctt ccgtaagctg gaagataagt tctgggtcaa cgcctgtctg gtccatctag
     2401 catacatcga ctgtgaggag ggggcgtacg gcgcggcccg ctcccgcctg ctgcagacca
     2461 acgatacgtt gcccctggta cagttcccgt ggggcgcgac gtacgtactg gatggcttct
     2521 cacgcctggc cgtggcgcaa ggagaatcgg tgcgggccct ccggctggga ggtgcgacgg
     2581 acgcgcttcg ccagacctac ggcgtgacga tcgggccaac cgagcaggcc gcattccggc
     2641 gccgcctgga gccggcccgg agagtactcg gcgaggaggc gggagacaga gcctgggaag
     2701 agggccgggc gatgacgctg gaggaggccg tggacctcgc cctcacggag accgggccgg
     2761 gccgtcgccc caacggcctc ctcagcgacc gtgagctcga agtgcttcgg ctcgtggcca
     2821 gcggattctc cgacgtagaa gtggccgaga acctctacct cagcccccgc accgtaggag
     2881 ggcatctggg gggtatctac cgcaagctcg cggtcaagag caggaccgcg gccgtcaaga
     2941 aggcgggaga actcggcctg atctaggccg ccatagtatc ccgcttcgcg cggtccacga
     3001 ccagcctatc cataccaggt aattttgcgc atgttgcgtg atcgggtagg tacaagtatt
     3061 tctgccgatg ctcgccgtct gcgatcggga tagcgtggtc ctggaagcga tcacgagacc
     3121 ccagaggagg cgaacgagca tgaccggcaa gacccatgac gttgcggtgg ttggggcgag
     3181 catcgccggt tgcacggcgg cgatattact agcacgggag ggggcgaggg tggccctgat
     3241 cgaacgccat agcgacccga acgcctacaa ggcgctatgc acccacttca tccagccgag
     3301 cgccgtgccg accatggaga ggctaggcct ccttcccttg atcgaagcgg ccggggcggt
     3361 gccgaacgag atagacg
//
LOCUS       DBNGVD010000479         3084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_662530, whole genome shotgun sequence.
ACCESSION   DBNGVD010000479 DBNGVD010000000
VERSION     DBNGVD010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_662530"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16CM_12850"
     CDS             <1..277
                     /locus_tag="AC16CM_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HIR3140768.1"
                     /translation="DWMEAFYEEASDQPNMFDLRGEESGYTKRANFDAIGRFFVERLE
                     LVFAGVSKNPLPLPNSKGVPLYLLCFAASNPKGVKPALRIANHLLQR"
     gene            complement(348..774)
                     /gene="rnpB"
                     /locus_tag="AC16CM_12855"
     ncRNA           complement(348..774)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16CM_12855"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(787..1167)
                     /locus_tag="AC16CM_12860"
     CDS             complement(787..1167)
                     /locus_tag="AC16CM_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140769.1"
                     /translation="MGINQRVSLRKFIDDIRRWVDEGRSDDWIASALGTSPSSVQSFR
                     SRNAIYRRGATSTLHDPQDFSSYEGVLEPTGPGVWFDPEIGSDPRWQKRWNSTERVEL
                     RLTPTRIVLLRRGKARSSLSAPEV"
     gene            1275..1565
                     /locus_tag="AC16CM_12865"
     CDS             1275..1565
                     /locus_tag="AC16CM_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140770.1"
                     /translation="MEILLNVGVGILRSIPLILLLYIPALLGMATWRERGPGYRVRTL
                     LWFGIGFGLIVAVHIMIRSVSVLQVLEVIGLSLVQVAVALGLARLTVYRLAD"
     gene            complement(1647..2840)
                     /locus_tag="AC16CM_12870"
     CDS             complement(1647..2840)
                     /locus_tag="AC16CM_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3140771.1"
                     /translation="MPEDLAVFIDWENIYISTVTEYKSKPNVSAILEKASEYGRIVSA
                     TAYADWTDGDFRQAPPTLYSNGISPRYISARYFPGGKSQKRRTNSIDVMLAVECSDFL
                     HGHPQVGTYVLVTGDGDFIPLVSLLRSKGKNVVVIGVSEATSYHLIESADHFISYASL
                     LEEDRAARAQDKAPKKEAADPYKELVRAVETLKRGGKTRVLGQVKQQIIVQLGSFDER
                     SAGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDEKVPDLQESDLAAEDPKEAQPAS
                     KTKNNGRPQREERQPEERQPEPGFDLLEDLTAYELKTVCNSVAGLQKPATFVEIFSSI
                     RDLDERGELELSREEIGEVINAMLEADYLNKVRQRPYKQAKADMTFYALNTDKNEVKN
                     VLNGG"
     gene            complement(2897..>3084)
                     /locus_tag="AC16CM_12875"
     CDS             complement(2897..>3084)
                     /locus_tag="AC16CM_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140772.1"
                     /translation="MESAEYSVYLDGTEEPVTIVAAFDLPRGFESGEQQLLWVEAVLD
                     EETLSQTVVRIHDRYSV"
BASE COUNT          516 a          946 c          942 g          680 t
ORIGIN      
        1 cgactggatg gaggccttct atgaggaggc tagcgatcag ccgaacatgt tcgatctacg
       61 cggcgaggaa tcgggctaca ccaagcgcgc caacttcgac gccatagggc gctttttcgt
      121 ggagcgcctc gagctggtct tcgccggggt ctccaagaat cccttgccgc taccaaactc
      181 gaagggcgtg ccactgtacc tgctatgttt tgcggcttcg aatcctaaag gcgtcaaacc
      241 ggctttaaga atcgcgaacc acctacttca gaggtagttt cgacgtcggt tagggtatag
      301 tttagagtct cagctcctct acggtacacg taaactccag atgtaaagat cggggccgtc
      361 ctgtaagccg gattctgctc tatcccgcca tccatctccg ggagattccc gggcttcccc
      421 gaggggaagc tgcgcctacc agtccagcgg ttcgcgaacc gcttttgcgg acaatttggc
      481 ttgctcgcac cggtggttta cctggccggt ccctccggct ttcgcctcgg gataccgccg
      541 gtgggctctt accccaccct ttcacccatc acctgtgacc tggtaggggc gcttcgcgaa
      601 gcgcccctac ccgatccatc ggctggtctg ctctctgttg cacttgacgt gacggggggc
      661 ttgcgccccg ccgcccctcg gcttgttggg ccgagcggcc tctgctgcga gtccggactt
      721 tgctctacgt ctcgggcaga aaccctgaaa cgcagcgacg ggtcggacgg cccggaccgt
      781 agggaattat acctcaggag ccgaaagtga gctcctcgcc tttccgcggc ggaggaggac
      841 tatcctggta ggcgtgagac ggagctcgac cctctcggtg ctgttccagc gcttctgcca
      901 gcgtgggtcg ctgccgatct cggggtcgaa ccacacgccc gggcccgtag gctcgagcac
      961 cccctcgtag gaagagaaat cctgcggatc gtgcaacgtc gaggtcgccc cacggcgata
     1021 gatcgcgttg cgcgagcgga acgactggac cgaagaggga ctggtcccga gggcgcttgc
     1081 gatccaatcg tcgcttcttc cctcgtcgac ccaacggcgg atgtcgtcta tgaactttct
     1141 gagcgagacc ctctgattaa tacccacgca cgccaccgcc ttgtagcctt caatgtgacc
     1201 gctcttactc tctagattac agacagagcg tctggttttc cagtaacacg gaacctgtgg
     1261 tgtagactcg tggcatggag atacttctga acgtaggcgt ggggatactg cgctctatac
     1321 ccctgatcct gctcctgtac atcccggctc ttctggggat ggcgacctgg agagagcggg
     1381 gccccggcta cagggtcagg acgctgctgt ggttcggcat tggcttcggc ctgatagtgg
     1441 cggtacacat catgatacgt agcgtctccg ttttacaggt cctggaagtg atcggcctct
     1501 cgctggtgca ggtggcggta gccctggggt tggcgcgact gacggtctac cgcctggctg
     1561 attagccttc agctatcacc cgtcagcaat cagctagaga caataagctg aaagctgatg
     1621 gttgatagct aacggctttc gaagcgtcag ccgccgttaa ggacgttctt gacctcgttc
     1681 ttgtccgtgt tgagggcgta gaaggtcatg tcggccttgg cctgcttgta gggccgctgg
     1741 cggaccttgt tgaggtagtc tgcctcgagc atggcgttta tgacctcgcc tatctcttcg
     1801 cgggagagct ccagctcgcc ccgctcgtcg aggtcgcgga tgctggagaa gatctccacg
     1861 aaggtggccg gcttctggag gccggcgacg ctgttgcaga ccgtcttgag ctcgtaggcc
     1921 gtgagatcct cgagcaggtc gaagccgggc tcgggctgac gctcttctgg ctggcgctcc
     1981 tcgcgctgcg gcctgccgtt gttcttggtc ttgctggcgg gctgggcctc cttcgggtcc
     2041 tcggccgcga ggtcactctc ctgcaggtcg gggaccttct cgtcaggcag gtagacctga
     2101 agctccaggt cctggtccac cgtgttcacc aggccgcggc gctccgcctc gaccacaaag
     2161 tccttgaact tcttgaagcc ggcgcttcgc tcgtcgaaag agccgagctg cacgattatc
     2221 tgctgcttga cctggccgag tacgcgcgtc ttgccgcccc tcttgagcgt ctccacggcc
     2281 cggaccagct ccttgtacgg gtcagcggcc tctttcttcg gtgctttgtc ctgcgcgcgg
     2341 gcggcccggt cctcctccag cagcgaggcg taggagatga aatggtctgc tgattcgatc
     2401 aggtggtacg acgtcgcctc ggagaccccg atgaccacca cgttcttgcc cttgctccgg
     2461 agcaagctga ccagcgggat aaaatctccg tcgcccgtga cgagcacgta ggttccgacc
     2521 tgcgggtggc cgtgcaggaa gtccgagcac tccacggcca gcatcacgtc tatggagttg
     2581 gtgcggcgct tctgggactt gccacccggg aagtagcggg cggagatgta gcgcggcgag
     2641 atgccgttgg agtagagggt tggcggggct tgccggaagt ccccatcggt ccagtcggcg
     2701 taagctgtcg ccgagacgat acgcccgtac tcgctagcct tctccagtat cgccgagacg
     2761 ttcggcttcg atttgtactc cgtgaccgtg gagatataga tgttctccca gtcgataaat
     2821 actgccagat cttcgggcat tgagtttttc ctaacgtaga agagaggccc ggtttcccgg
     2881 gcccctgttg gtcaatttat acgctgtagc ggtcgtggat tctgaccacg gtctgcgaga
     2941 gcgtctcttc atccaggacg gcctcgaccc agagcaactg ttgctccccc gactcgaacc
     3001 cccggggcag gtcgaaggca gccactatcg tcaccggctc ctccgtcccg tcgaggtaga
     3061 cggagtactc ggccgactcc atga
//
LOCUS       DBNGVD010000480         8274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_300711, whole genome shotgun sequence.
ACCESSION   DBNGVD010000480 DBNGVD010000000
VERSION     DBNGVD010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_300711"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1129)
                     /locus_tag="AC16CM_12880"
     CDS             complement(284..1129)
                     /locus_tag="AC16CM_12880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3140773.1"
                     /translation="MSGRRIRVMSFNVRGSFHDLTRKNAWRNRAQLNVATIRRLAPDV
                     IGLQESQRGNLATYRRHLPGYAQIRGPRYGNAFPCDSNTILYDPERVMPLDVGGFWLS
                     ETPERRSRSWGTRVARSATWALFVPKNTGLSILHLNTHLDHVSAPARREGSKLLVARV
                     SELTDRAGETPAAIVTGDFNARPGSATHKILTDSGFVDTFLAAGNEEDQGANTFHNFQ
                     GTGYCDEHPAKGPRRIDWILLKDPETRLTTESHSIIRDTEEEAGLYPSDHYPIVVDFG
                     LAVTF"
     gene            1831..2478
                     /locus_tag="AC16CM_12885"
     CDS             1831..2478
                     /locus_tag="AC16CM_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140774.1"
                     /translation="MNFFEYMSYSWDHVLELAIGHALVVAISLVIAVVIGVSLGVLVY
                     RRERIANVVLAITSTFLTIPSLALFGVFIPIFGLGYLPSVVALVMYALLPILRNTVTG
                     LQSVDPAVAESAQGMGMSARERLLRIELPLAWPVIIAGVRVSTMLLMGIAAIAAVVNG
                     PGLGDDIFAGLARIGGANALNLVLGGTLGIIVLALLFDTFFNVLGRLTTSRGIRD"
     gene            2471..3649
                     /locus_tag="AC16CM_12890"
     CDS             2471..3649
                     /locus_tag="AC16CM_12890"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140775.1"
                     /translation="MTEEAPKRQNSQTAETMIYLEDLSKVFPAQSEPAVDDLSMEIYE
                     GEIVVFVGPSGCGKTTTMKMINRLIEPSGGRIFLEGEDVTGVNPDHLRRRIGYVIQQI
                     GLFPHMTIADNIATVPRMLGWEKDRISGRTDQLLEMVGIDRSYRDRYPKELSGGQRQR
                     IGVARAMAADPPVLLMDEPFGAIDPITRDRLQDEFLRLQQEIKKTIIFVTHDIDEAIK
                     MGDRIAILKQQSKIAQYDTPEKILTDPADDFVEDFIGAGASIKRLRLSTVGEIETADW
                     PVARLSDSREKIREKVRASSRDFVLLTDDRDRPKRWVSAEHLERNTPIEEAGQPAVAI
                     VGADSSLYNTLDTMITSYKGSAILVGDDGTYRGVVDFDAVLAAINAMRPREQQIHEEA
                     "
     gene            3653..4417
                     /locus_tag="AC16CM_12895"
     CDS             3653..4417
                     /locus_tag="AC16CM_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140776.1"
                     /translation="MTAYKSSTGMSGGTRRLLRYLGMPILLAFLCLAMYLYIGSKDLD
                     SIERRQINAEVITARLVEHIQLALVSTVIVVVLAVGVGILLTRPFARRVSTPIIGVAN
                     IGQAIPSIGLLALLAIVWTGGFWPAVVALVAYSFLAILRNTMVGLQQVDHSTIEAARG
                     MGMTKTAVLFRIELPLAVPIILAGIRTALIINVGTATLTTFTGAGTLGELINNALRLG
                     REDTVLVVGSGLTAILALSIDWLAGIAEDVLRPKGL"
     gene            4466..5419
                     /locus_tag="AC16CM_12900"
     CDS             4466..5419
                     /locus_tag="AC16CM_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3140777.1"
                     /translation="MQKKRAKLMRLLGIVTVLALIAAGCGGGGGGGATGDIDLSDLEV
                     TVGSKEFTEQLILGQITVQALEAAGATVEDQTGLAGSVAARKALESGEIDMYWEYTGT
                     GWITYLQHTKPIPNSKKQYEAVAKEDLKENGIQWLSPAPANNTYAMAVRSEAYNDLGV
                     KKLSDFKQLVEENPEDATVCVGTEFSTRDDGLPGMEKAYDFQFPSDNVVKIDEGQIYQ
                     ETDRGNRCNFGEVFQTDGRIANLDLELVEDDEGFFPIYNPSLNVREQLIEENPQVEKL
                     FAPISKKLTTSELQKLNAKVDVQGQLEDQVAEEWLSENGFI"
     gene            5858..>8274
                     /locus_tag="AC16CM_12905"
     CDS             5858..>8274
                     /locus_tag="AC16CM_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR3140778.1"
                     /translation="MGVVVARSEEDVKLALSAARGLGLSITPRGTGTSLAGQATAPGL
                     ALDVSEMRRALEIDFQGRRCVVEPGLVQGELNAMVAPRGLVFGADTSTSDVATLGGMV
                     GNNSAGMRSLVYGMTSDQVVGLRCVLASGETVDLEPLDRDAARKRARHADATARLLRG
                     VMEIGERYGEEIRRRFPRMIRRVSGYGLDALVDPDTLDLTRLICGSEGTLAVVTRAEF
                     RLHPLPETRALASFEFDSLVGAARATGQFLETDPSAIELLDDVAIDRARANPAYRDST
                     RFVRGEPKAILIVEWSGTEREIDERFADLDELAGEVGASAATPLRTKTEMDQTVKLRK
                     STLPLLLGTTDEAKPVAFVEDAAVPADRLEEFVARFGEIVEDNGTWACFYGHASVGCL
                     HVRPALDTSDPEGVSRMRRIAEEVADLVVDCGGSISGEHGDGLSRSEFLEGMYGPEIL
                     RAFGEVKRLFDPGGMMNPGVIVDPPRMDRQLRIGPGRRRLPLKTNLSFEGQGGFAKAV
                     ELCNGSGLCRKRTGGTMCPSYMVTLDEQDTTRARANMLRSVIEGTLPPEELTGERMKE
                     VMDLCVGCKACKTECPSAVDVASMKTEVLYQTGRRHGFSPRQKAAGHIRRQLALAALM
                     PSLYNAVAGSLPARRVAGLAGIDPRRSLPRVTNRTFSRRFPDLPQGTGPAEVALFNDT
                     WNEYQRPEVGEGAVRLLAAAGARVHLPQVVCCGRPMLSEGLVDEARRNAKRNLDLLMP
                     LVERGVPLVGLEPSCILTMRDDYRKLLPEDERVEKLAEATRLFEEALLGLDAELRFRE
                     GDPVLLHG"
BASE COUNT         1470 a         2615 c         2728 g         1461 t
ORIGIN      
        1 gagctcggta tggtcctgcg cggcgctgct tagagcggct cgcttcgcga accgcgccgt
       61 cagctactag ctgtcagctt tcgggctgaa gacgcgcccg gtgtaagggc ctaagcctca
      121 agttcgaccc tgagaaaggg agccccgttt tcggggctcc ctttcttcgt ttcgtggggg
      181 gcgtttatga gtccctgtgg cagatggcag aggaacgtgt cttctcgaag actgccatca
      241 acctaaagtt cctggctgat agctgacggc tgatagctga cggctaaaaa gtgacagcta
      301 atccaaaatc tacaactatc gggtagtgat cgctcgggta gagccccgct tcttcttcgg
      361 tgtcgcggat gatgctgtgt gactccgtcg tcagcctggt ctcggggtct ttgaggagga
      421 tccagtctat ccgcctcggc cccttcgcgg ggtgttcgtc gcagtagcct gtgccctgaa
      481 agttgtggaa cgtgttggcg ccctgatctt cttcgttgcc tgcggcgagg aaggtgtcca
      541 cgaaaccaga gtccgtgagg atcttgtggg ttgcgctgcc cggcctggcg ttgaagtctc
      601 ccgtcacgat ggccgcgggg gtttcgcccg cgcggtcggt tagctcggat accctggcta
      661 cgaggagttt gctgccttcc ctgcgggccg gggcgcttac gtggtcgagg tgggtgttga
      721 ggtggaggat ggagaggccc gtgttcttcg ggacgaaaag agcccacgtg gcggagcggg
      781 ccaccctcgt gccccagctc ctggagcgcc tctccggggt ctcggagagc cagaatcccc
      841 cgacgtccag cggcatgacc cgttccgggt cgtagaggat ggtattcgag tcgcagggga
      901 aggcgttgcc gtatctcggg ccacggatct gggcgtagcc gggcaggtga cggcggtagg
      961 tggccaggtt gccgcgctgg gactcctgga gcccgatcac atcaggcgcg aggcgcctta
     1021 tggtggcgac gttcaactgg gcccggttgc gccaggcgtt ctttctggtc aggtcgtgga
     1081 acgagcccct gacgttgaag ctcatcacac ggatccggcg cccactcacc ccggggtcct
     1141 gctcctactc gggtttcttg agtatcgggc gcacgggttc tccggcctcg tgtcggcgca
     1201 ggatttcctc catctgctgt tatctacgga gtcgctactc ccactcggtt gccgccacgc
     1261 cggatgacga gcggagatca cgccggtacg tttccaggcg cagtctagca tcgacgctca
     1321 tcccgcaaca atgcgacgtt gcccccaacg cacacttggc aaccagcgtt gcacacgtac
     1381 ttgtgcgtgc taaggcccgg taggcattct gtgcatgccg ggtgaccagg gccccgccgg
     1441 accggccgag aggccgtcac caaacgtcgg cgatgagcat tgtgatatcg tcggatctca
     1501 cccccacgct cagcgcgtgg cggctggaaa ctggttttgg gtgtccaaag ccgttaatat
     1561 cgggacgaag ttgcagccct tgctgctcgg gagatcatcg cgaaggaggt ggcggggagt
     1621 atgtatatag ttatgatggt agcattcgag tccggtgttc gtggtactag cggcggaagg
     1681 aacttgccac tttgacggtt cgtggcgagt acgaagcggt cgagaacagg gattctgggg
     1741 cgcggagtcg gcgaaccctt ccggacttcc tgacgtgcga ccttgccccc gttctgacct
     1801 ctgcagagag tttggaaggg agggggtagc atgaacttct tcgaatacat gtcctacagt
     1861 tgggaccatg tgctcgagct ggccataggg catgcgctgg tcgttgccat atcgttggtc
     1921 atcgccgtgg ttatcggggt gagcctgggc gtgctggtgt accgtaggga gaggatcgcg
     1981 aacgtggtgc tggcgatcac ctcgaccttt ctgaccatcc cttccctcgc gcttttcggg
     2041 gtcttcatac cgatcttcgg cctcggctac ttgccctcgg tcgtcgcgct ggtgatgtac
     2101 gcgctcttgc ccatcttgcg caacacggtc accggcctgc agtccgtaga ccccgccgtc
     2161 gccgagtccg cccagggaat ggggatgagc gcccgggagc ggttgttgcg catcgagctc
     2221 cccctggcct ggccggtcat catcgccggg gtgcgtgtgt ccacgatgct cctcatgggc
     2281 atcgcggcca tcgccgccgt cgtcaacggt ccgggcctgg gcgacgacat cttcgcgggg
     2341 ctcgcccgca tcggcggtgc caacgccctc aacctcgtgc tcggcggcac gcttggcatc
     2401 atcgtgctgg cgctcctctt cgacaccttc ttcaacgtgc tgggtaggct aacgacttcg
     2461 aggggaatcc gtgactgaag aagcgcccaa gcgtcagaat tcgcagaccg cagagaccat
     2521 gatctacctg gaggacctct ccaaggtctt cccggcccag agcgaaccgg cggtcgacga
     2581 tctctccatg gagatctacg agggagagat cgtggttttt gtcggcccgt ccgggtgtgg
     2641 caagaccacg acgatgaaga tgataaaccg cctgatcgaa ccctccggcg gcaggatctt
     2701 tctggagggc gaggacgtga caggggtgaa cccggaccat ctccggcgcc ggataggcta
     2761 cgtgatccag cagatcggtc tcttcccgca catgaccatc gcggacaaca tcgcgacggt
     2821 gccgcggatg ctcggttggg agaaagaccg catctcgggc cggacggacc agctgctaga
     2881 gatggtgggc attgaccgct cctaccgtga ccgttacccg aaggagctgt cgggggggca
     2941 gcgccagcgc atcggggtgg cccgcgcgat ggccgccgac ccgccggtgc tgctgatgga
     3001 cgagcccttc ggggccatag accccatcac gcgcgacagg ctacaggacg agtttcttcg
     3061 cctgcagcag gagatcaaga agaccatcat cttcgtcacc cacgacatag acgaggccat
     3121 aaagatgggc gatcgcatag cgatcctcaa acagcagtcg aagatcgccc agtacgatac
     3181 ccccgaaaag atcctgaccg atcccgccga cgacttcgtc gaggacttta tcggggctgg
     3241 ggcgtccatc aagcgcctgc ggctcagcac ggtcggcgag atcgagacgg ccgactggcc
     3301 ggtcgcgagg ctctccgaca gccgcgagaa gataagggag aaggtaaggg cctccagcag
     3361 ggacttcgtg ttgctgaccg acgaccggga tcggcccaag cgctgggtca gcgcggagca
     3421 cctcgaacgt aacaccccga tcgaagaggc cggccaaccg gcggtggcga tcgtgggcgc
     3481 cgattcgagc ctgtacaaca ccctggacac tatgatcacg tcctacaagg gctcggcgat
     3541 cctggttggc gacgacggta cttacagggg cgtcgtggac ttcgatgccg tgctggcggc
     3601 gatcaacgcc atgcgtcccc gggagcagca gatccatgag gaggcgtagg agatgacggc
     3661 gtacaagtcc tcgacaggaa tgtccggggg caccagacgt cttttgcgct acctcgggat
     3721 gcccatcctc ctcgcgttcc tgtgcctggc catgtacctg tacatcggca gcaaagacct
     3781 cgacagcatc gagcgacgcc agatcaacgc ggaggttatc accgcccggc tggtcgagca
     3841 catccagctc gccctcgtct ccaccgtgat cgtggtggtg ctggccgtcg gcgtgggcat
     3901 actgctcacc cggccgttcg cccgacgggt gtcgacgccg ataatcggcg tggccaacat
     3961 aggccaggcg atcccttcca tcgggctgct cgcgctcctt gccatcgtct ggaccggtgg
     4021 gttctggccc gccgtcgtcg ccctggtagc gtactcgttc ctggccatcc tgcggaacac
     4081 catggtgggg ctgcagcagg tagaccactc gaccatcgag gccgcccgtg gcatggggat
     4141 gaccaagacg gcggtgctgt tccgcatcga gctgccgctc gccgtgccca tcatcctcgc
     4201 cggcatcagg accgccctga tcatcaacgt cggcacggcc acgctgacga cgttcacggg
     4261 cgccggaacc ctcggggagc tgatcaacaa cgccctccgc ctgggccggg aagacacggt
     4321 gctggtggtc ggcagcgggc tgaccgccat cctggccctg tccatcgact ggctcgcggg
     4381 catagccgag gacgtcctgc gcccgaaagg cttgtagacc gttaccgtcg tgaaaccctc
     4441 aaccgatccc ataaggagga cggccatgca aaagaaacgt gcaaagctga tgcgactgct
     4501 aggaatcgtc acggttctgg ccctgatcgc cgccggttgc ggaggcggtg gaggaggggg
     4561 agcaacgggg gacatcgacc tctccgatct ggaggtcacc gtcggctcca aggagttcac
     4621 cgagcagttg atcctcggcc agatcaccgt ccaggccctg gaggccgccg gggcgacggt
     4681 ggaagaccag actggccttg ccggatcggt cgccgcccgc aaggcgctgg agtccggcga
     4741 gatcgatatg tactgggagt acacggggac cggctggatc acctacctgc agcacaccaa
     4801 gcccataccg aacagcaaga agcagtacga ggctgtggcc aaagaggatc tcaaggagaa
     4861 cggcatacag tggctctccc cggccccggc caacaacacc tatgcgatgg ctgtgcgcag
     4921 cgaggcctac aacgacctcg gcgtcaagaa gctctcggac ttcaagcaac tagttgaaga
     4981 gaaccccgaa gacgccaccg tgtgcgtggg gaccgagttc tctacccgcg atgacggcct
     5041 gccggggatg gagaaagcct acgacttcca gttcccgagc gataacgtcg tcaagatcga
     5101 cgagggccag atctaccagg agactgacag gggcaaccgc tgcaacttcg gcgaggtgtt
     5161 ccagaccgac gggcgcatcg ccaacctgga cctcgaactg gtcgaggacg acgaggggtt
     5221 cttccctata tacaatcctt ctctcaacgt gcgcgagcag ttgatcgaag agaacccgca
     5281 agtcgagaag cttttcgcgc ctatctccaa gaagctcacc accagtgagc tgcagaagct
     5341 gaacgccaag gtcgacgtgc agggtcagct cgaggaccag gttgccgaag agtggctcag
     5401 cgagaacggc ttcatctagg agacatcccg acagcggttg ccggacacgg ggtcaggcaa
     5461 ccgctgtact cttggtatct ttaccaggcg accatcgctc tcctgcacag gaactcgcgt
     5521 ggcttgcgca tgatagatgc gtcgctggtc cccgaagtgc cccgttcctc cttgggtacc
     5581 ctcccggcat gacaacatgg tcggcgtgcg aacaaacgag gaacaacacg ggtagccctg
     5641 gccggggaac gggatcgcgc cgccaaagac ggcggcgacc cccgagtggc ggtaaactct
     5701 ccctggttgt agggtcgacc gagagacggt tatcatcgaa cgttctgcaa aacaactcca
     5761 tgcgtccctc gacggtcggc tagaaggtta cctgcgcacc gacgaggcct cgcgtgccct
     5821 gttctccacc gacgcctcca tctacctgcg caagcccgtg ggcgtcgtgg tcgcccgctc
     5881 cgaagaggac gtcaagctag ccctctccgc ggcccgcggg ctgggcctct ccatcacgcc
     5941 ccgcggcacg ggcacgagcc tcgccggtca ggcgaccgcg ccgggcctcg ccctcgacgt
     6001 ctctgagatg cgccgggcgc ttgaaataga ctttcaggga cggcgttgcg tggtcgagcc
     6061 gggtctcgtg cagggcgagc tgaacgccat ggtcgcgccg cgcgggctcg ttttcggggc
     6121 cgacacctcc acctccgacg tggccacgct cggcggaatg gtcgggaaca actccgccgg
     6181 gatgcgcagc ctcgtctacg ggatgacctc ggaccaggtc gtcggccttc gttgcgtgct
     6241 ggcctccggg gagacggtcg acctggaacc gcttgaccgg gatgcggcca ggaagcgggc
     6301 ccgtcacgcg gacgctacgg cgaggctgct caggggcgtg atggagatcg gggagcggta
     6361 cggggaggag atccgacgcc ggttcccgcg catgatccgt cgcgtctcgg gctacggcct
     6421 ggacgccctc gtcgaccccg acacgcttga ccttacgcgc ctgatctgcg gttcggaggg
     6481 aacgctggcc gtcgtcaccc gcgcggagtt ccggctccac ccgctccccg agacccgtgc
     6541 ccttgcctcc ttcgagttcg attccctcgt cggggccgcg cgcgccacgg gacagttcct
     6601 ggagacggac ccgtcggcga tagagcttct ggacgacgtg gccatcgacc gggcgagggc
     6661 caaccccgcc tacagggact cgacccgctt cgtgcgggga gaaccgaagg cgatcctcat
     6721 cgtggagtgg agcgggacgg agagggagat cgacgagcgt ttcgcggacc tcgatgagtt
     6781 ggccggcgag gtgggggcta gtgccgccac gccgctacgc acgaaaacgg agatggacca
     6841 gacggtcaag ctccgcaagt cgacgctccc gctgctgctc ggcaccacgg acgaggctaa
     6901 gcccgtcgcc ttcgtggagg acgcggcggt gccagccgac cggttggaag agttcgtagc
     6961 ccggttcggg gagatcgtgg aagacaacgg tacctgggcc tgcttctacg ggcacgccag
     7021 cgtgggatgc cttcacgtgc ggcccgccct ggatacctcc gacccggaag gtgtctcgcg
     7081 catgcgccgc atcgccgagg aggtcgccga cctcgtcgtt gactgcggcg gttccatctc
     7141 gggcgagcac ggcgatggcc tctcgcgctc ggagttcctg gaggggatgt acgggccgga
     7201 gatcctgcgc gccttcggtg aggtcaaacg cctcttcgac cccgggggga tgatgaaccc
     7261 cggcgtcatc gtggaccccc cacggatgga ccggcaacta cgcatcggcc ccggcaggag
     7321 acgcctgccc ctgaagacga acctctcctt cgagggccag gggggcttcg cgaaggccgt
     7381 cgaactgtgc aacgggagcg ggctctgccg caagaggacc ggcggcacga tgtgcccgtc
     7441 ctacatggtc accctcgacg agcaggacac cacgagggcg cgggcgaaca tgcttcgctc
     7501 cgtgatcgaa ggtaccctcc cgccggagga gttgaccggg gaacgaatga aggaggtgat
     7561 ggacctctgt gtgggctgca aggcgtgcaa gacggaatgc ccctcggccg tggacgtggc
     7621 ctccatgaag acggaggtcc tctaccagac gggccgaaga cacggcttca gcccgcgcca
     7681 gaaagcggcc gggcacatcc gccgccagct cgcgctcgcg gccctgatgc cttccctcta
     7741 caacgccgtc gctggctccc tccccgcccg cagggtcgca ggcctcgccg gcatagaccc
     7801 ccgccgctcg ctgccgcgcg tgacgaacag gaccttctcg aggcgcttcc cggacctgcc
     7861 gcagggtacc ggaccggctg aggtggccct cttcaacgac acctggaacg agtaccagcg
     7921 gcccgaggtc ggggagggag cggtgaggct tctagcagcc gctggtgcca gggtgcatct
     7981 gccgcaggtg gtctgctgcg gcaggcccat gctctccgaa gggctcgtgg acgaggcaag
     8041 gaggaacgca aagcgcaacc tggacctgct gatgccactg gtcgaaaggg gcgtccccct
     8101 ggtcggcctg gagcccagtt gcatcctgac catgcgcgac gactaccgca agctcctccc
     8161 cgaagacgaa cgcgtcgaga agctggccga ggcaacgcgc ctcttcgaag aggcgctgct
     8221 tgggctggac gcggagctac gctttcgcga gggtgacccc gttctattgc acgg
//
LOCUS       DBNGVD010000481        12981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4308964, whole genome shotgun sequence.
ACCESSION   DBNGVD010000481 DBNGVD010000000
VERSION     DBNGVD010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4308964"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="AC16CM_12910"
     CDS             complement(<1..314)
                     /locus_tag="AC16CM_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140779.1"
                     /translation="MAGFDIRDMMNDIDEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGLGEDDLPELVKMCTGCSLCWDFCPRGGLQYESTWKITGNGESVEGIGRVEE
                     SYT"
     gene            complement(314..1966)
                     /locus_tag="AC16CM_12915"
     CDS             complement(314..1966)
                     /locus_tag="AC16CM_12915"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3140780.1"
                     /translation="MRFLREDQTLNNVERIKQARHPLEVRQAIIDTYSKDLSAMDEVP
                     GEVERLKWVGIYPQKQGGDAYMMRVKIPGGVLTPAQTEVIGRIASEFAHGPIPNPHFG
                     NNFLDLTTRQDVQMHWLRMGDIPEIWRRLEEVGITTVQACGDSARNVLCCPVSGLDHD
                     EVIDAFPVAQAISSYFTGNREYANLPRKFKLSVTGCLEDCAQAEINDVGLLPARLQDG
                     TLGFNLRVGGGLSDGPRMASDIDVFVRPEEAVEITRGIAQVFGELGNRENRWTARMRY
                     LVQELGPEGFREELARRVSVDLTPAGEDLTRRYRGDHVGVHPQKGRDDSGTDLHYVGL
                     NVPVGRMGGEQFEEAGRLAREYGAGVRLATDQNLVITGVPGERLDDLLAEPLLERFSP
                     NPGAFERGVVACTGSEFCRFGIVETKVRAVEWAREMDERVGDVGQEAVRMHFSGCSAS
                     CAQPQIGDIGFRGETAKTKEAIVEGVDIGLGGSLGRDAAFIDWVEGSRPTEEVPDALV
                     AVFEKFEEERREGERFHEWARRKRNAELREALRKPVRSGVSA"
     gene            complement(2008..2766)
                     /locus_tag="AC16CM_12920"
     CDS             complement(2008..2766)
                     /locus_tag="AC16CM_12920"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR3140781.1"
                     /translation="MHESLRSKRNDRILPGLEALERESDRLEGADPREVIAWAVETYG
                     EGLALSASFGGGEGMALLDMISSVTDKVTVLTIDTGFIFKETAEFREEVMRRYKLPVE
                     VLRPAMTIEEQVERYGEQMRTCTPNLCCQVRKIEPLQRALTRYDAWMTGIRREQTPQR
                     AGTRVVAWEERYEAAKIAPMPHWTETQVWDYVRSHDVLVNPLLHQGYRSIGCEPQTRP
                     VHDDEDPRAGRWSEMDKTECGIHFEDGRVSRAGA"
     gene            complement(3011..4261)
                     /locus_tag="AC16CM_12925"
     CDS             complement(3011..4261)
                     /locus_tag="AC16CM_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140782.1"
                     /translation="MNRLGAYPIYLFIKGSFALFFTLWSTVAPVYRIEVVGLDPLRLV
                     LLGTALEVAVFVFEVPTGVFADTFGRRRSVIVGCVLMGSGFALEGAIPEFSAVLAAQA
                     VWGVGYTFISGALEAWIADEAPDRDLGRVYIRGEQADYLGSFLGIPGGVLLGLVALNL
                     PLLAGGVLTVALGLALILAMPERNFRPSPREGRSSVEHAAATARGGVRLVRSRPILLI
                     LLAAALFTGMSEEGFDRLNPKQFLDVVGLPTIGGLEPVVWFGAIGAGGLVLSYLAAGI
                     VARAIDVGNPAVAARLLLVLDLLLVAGMLTFALAGSFALALGAFWFATLVRRVAEPVY
                     LTWLNEGLDPAVRATVISMSSQAGALGEASAGPVVGVIGNVFGVRQALTVAALVLSPT
                     LLLYARAIKRGGAEPELGDNTPEK"
     gene            complement(4509..5243)
                     /gene="cofE"
                     /locus_tag="AC16CM_12930"
     CDS             complement(4509..5243)
                     /gene="cofE"
                     /locus_tag="AC16CM_12930"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR3140783.1"
                     /translation="MQVLSVEGFPEVLPGDDLDRMIRQAVAGDLSAGDVLVVTHKIVS
                     KAEGRIQDLGTVEPSALAKDYAARHGKDARQVEVVFRESRRIVRMDRGIVISETHHGF
                     VCANAGVDASNVPGDDTVCLLPVDPDASAAKLRGTLATELGVEVAVIISDSFGRAWRH
                     GITDVAIGVAGMDPIADYRGLNDPHGYPMEASVLAVADELAAAAELVMGKTEGIPLAI
                     VRGYPYSPASGSAGDLLMPPERDMFR"
     gene            complement(5249..5860)
                     /gene="cofC"
                     /locus_tag="AC16CM_12935"
     CDS             complement(5249..5860)
                     /gene="cofC"
                     /locus_tag="AC16CM_12935"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR3140784.1"
                     /translation="MSLTSGVSAVVPVKDLQGTKSRLSPILNPGARAGLTLYMMGRVV
                     AAIRSSGVEDVCVVSPDRIVLNEARRRGAAPLVQESRGLNPALEEGRRRAIDLGASTL
                     LVFPADLPLLEAEDVRAVLEASEGQAVVISPDGARFGTNALLTRPPDALPFAFGPGSF
                     GMHLEDARKRGLAVRVCERPHLAFDLDTAGDLAMLKKNGVARP"
     gene            complement(5857..6798)
                     /gene="cofD"
                     /locus_tag="AC16CM_12940"
     CDS             complement(5857..6798)
                     /gene="cofD"
                     /locus_tag="AC16CM_12940"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR3140785.1"
                     /translation="MKVVALAGGVGGAKLAAGLQSVLPAGDLSVVVNTADDFDLWGLH
                     ICPDLDTVMYTLAGIANPETGWGIANESFESLNMLDHYGEDTWFKLGDRDLATHVLRT
                     SRLRSGETLTEVTDALRGALGIESTVLPMSDDPVATVLDTPTGRLEFQEYFVRRRQKD
                     EVLGVEFLGIDDAGPTDEVFEAISGAEAIIFCPSNPVVSVGPILAVPGMREALASSRA
                     PKVAVSPIVGGRALKGPADRMLLSLGHEVSATGVAAMYSGLVDGMVVDSVDGEERGGI
                     EKSGVRVLVTDAVMRDDGDRARLAGETLEFGLGMVAR"
     gene            complement(6826..7398)
                     /locus_tag="AC16CM_12945"
     CDS             complement(6826..7398)
                     /locus_tag="AC16CM_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3140786.1"
                     /translation="MEGEATDNPWRTLGSRCVYENPWISVREDSVIRPDGEPGIYGVI
                     HYKNTAVGVLPVEDDHVYLVGQYRYPLERYSWEIPEGGCPEGEEPLEAARRELKEETG
                     LVARRWTRLGEAFLSNSVADEHAVWFLATDLIAGEPQNEGTEVIGVRRVPLREAVAMT
                     TDGRISDALSILALTNYALEKLGSSDAAAI"
     gene            complement(7484..8071)
                     /locus_tag="AC16CM_12950"
     CDS             complement(7484..8071)
                     /locus_tag="AC16CM_12950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140787.1"
                     /translation="MPPEEMDLVAPEARLETGRLLLEPILPAHAPALYERMQEERLYR
                     FIPQDPPNTPQALENRYEFLSARRSPDGREAWLNWAVREKRSGDYVGTLEATVEASEP
                     AFIAYMVFAPYQRQGFAAEACGRLLEHLFADYGVGVVAAEIDTRNVASIALVESLGFE
                     RVGFQKDADRFKGSTSDEYRYEIQHGAWSRGRQST"
     gene            8153..8995
                     /locus_tag="AC16CM_12955"
     CDS             8153..8995
                     /locus_tag="AC16CM_12955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140788.1"
                     /translation="MAEGRRRLTYPDREFVKRQYRDASRLNACIGLHERFSTNPQGLQ
                     DWVFDHFDIPEEASILDVGCGSGLLWRKNLNRVPREWCVTLTDASPGMLDEARADLSA
                     DQRFGFWIADIQHLPSVDGCFDAVIANHMLYHVPDRPRAISEISRVLRPGGTLYATTN
                     GREMHKEMGWMQRVLDPARPSDARFSDPLGFSLENGADQLSACFTDVTMKRYSDALAG
                     TEVDPLVDYLLSESSADAAERRTDAEEFERRVGELVELLEHELDSQGAIHITKDTGLF
                     VARN"
     gene            8982..9752
                     /locus_tag="AC16CM_12960"
     CDS             8982..9752
                     /locus_tag="AC16CM_12960"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3140789.1"
                     /translation="MRATRDMEILVGAPLDDPEVGFGLQQAVLEEVAAGERGPAALIW
                     TSSRYVGATRRETRLSGFALASEAAMELGFPVLVRNSGGGAVAANMGSLSFSLTFPVE
                     DLRQGLYERYSEGLELIASALRRVGVEAERGEVEGEFCPGAYSVRSGGPGGIKHAGLA
                     QRVTRRAARLEALLLVSETDEVRAVLERFYGLLGLPFRPGSVGDLPVDVPTVIRALSD
                     EARARYGARGVGIGEETLQRARALRNGWRFIPDSGSSL"
     gene            9770..10384
                     /locus_tag="AC16CM_12965"
     CDS             9770..10384
                     /locus_tag="AC16CM_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3140790.1"
                     /translation="MEYVFNAFVTLLVIIDPLGLAPIFAALTRGFSEKQRRESAIRGT
                     LLGGLILYVFALAGDVLLEALGIGLPAFRIAGGILLFLLSLDMVFARPSGMRASAVRE
                     QEEEEYQQDISVFPLAIPLLAGPGGITTVLLFTGGASVGQQALFMTVLFVVLVMTLAC
                     LLLAPRVMRLLGETGSNVLTRVLGVLLAALSVQFVIDGLQTSLA"
     gene            10496..11233
                     /locus_tag="AC16CM_12970"
     CDS             10496..11233
                     /locus_tag="AC16CM_12970"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3140791.1"
                     /translation="MSNAPIVIVGGATWWPNSYRDLANTLRDISGSEVHVAPITPLDW
                     ALGYLKGFGQLVFEVASTVDRALLEAETKKAVLVGHSAGGLACRVYIGGDPPYGGRRY
                     SGHRRVERLISLGTPHNVGQTKRLAPISRINDLFPGALHAEDGLRYLSVAGNVVDGAT
                     SAKARKRYERFVEDGRVGGDGVVPIEAALLPGSEEVVLDGIHHNRRMGSWYGSDAETV
                     GRWWPEALRTDGGPRTVRAAERSNNAG"
     gene            11223..11849
                     /locus_tag="AC16CM_12975"
     CDS             11223..11849
                     /locus_tag="AC16CM_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3140792.1"
                     /translation="MPADGADERQYSWEVAGADADALRSAMSHFPTGVTVVTSGQEEN
                     VEGMTANAVMSVSLDPVLFLISVHKDARINPRIREEGYFAISLLADDQEGLSRLFASP
                     ERSSGLPALHSLGGGYGSTGAPLAAGALAAIECELEDTYYGGDHDLFLGRVVGVRLGD
                     TRKGPLLFHEGAYPTLKSPSRPEDSGAFVDSVRGFDARMNRLRDRRRR"
     gene            11850..12674
                     /locus_tag="AC16CM_12980"
     CDS             11850..12674
                     /locus_tag="AC16CM_12980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140793.1"
                     /translation="MFDRNLLPIVRPLPVDAEGLVFVEGTTKDGMIESGEARSPTTGE
                     TYEIRNGYLDLLGSRIGADSIANLTNFLPGAGRGYEPLWRVRSLRLLTGEPFPNEQEL
                     RIIEDLVHQAPDGRYLDLGCSAGLYTRGLARDRNTSVVGIDISPSMLREADRRARQTE
                     TRPSFLRANAQNLPFFDGVFSGAVCGGSLNEFKNPARVLRETHRVLQSGGRLAIMGIL
                     RAQTRRGRRLQQLASTGGIRFFDPDELGSLLDHAGFEPDPLRTNGPVFLAGATRRG"
     gene            complement(12694..>12981)
                     /locus_tag="AC16CM_12985"
     CDS             complement(12694..>12981)
                     /locus_tag="AC16CM_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019764566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3140794.1"
                     /translation="PALLELGPKSAVITLGREGAVFSDGDSTGHVTSPEVEVVDTTGA
                     GDAFVGALASRLARDASLEDAVAYAARAGAAAVTREGAQGALPTPESVEAL"
BASE COUNT         2158 a         4388 c         4178 g         2257 t
ORIGIN      
        1 gtgtagctct cctcgacgcg ccctatgccc tcgacgcttt ccccgttacc ggtgattttc
       61 cacgtcgact cgtactgcaa cccgccacgc gggcagaaat cccagcacag cgagcagccc
      121 gtgcacatct tgacgagctc gggaaggtcg tcttcaccca ggccgatgga gtcggtcggg
      181 caggcagcga cgcacacgcc gcactgcacg cagcggtccg cgtcgataac ggccttctcg
      241 agatcccaga accagacctt gccgggggcc tcgtcgatgt cgttcatcat gtcgcggata
      301 tcgaagcccg ccatcaggcc gacaccccgc tccgcaccgg ctttctgagg gcctcgcgca
      361 gctcggcgtt cctcttgcgc ctggcccact cgtggaagcg ctccccctcg cggcgctctt
      421 cctcgaactt ctcgaacacg gcaacgaggg cgtccggaac ctcttcggtg ggcctggagc
      481 cctccaccca gtcgataaac gcggcgtccc tgcccaggga gcccccgagc ccgatgtcca
      541 cgccctcaac gatggcttcc ttcgtcttgg cggtctcgcc gcggaagcct atgtccccga
      601 tctggggctg cgcgcaggac gcggagcagc cggagaagtg catccttacg gcctcctggc
      661 cgacatcacc gacccgctcg tccatctcgc gcgcccactc gaccgccctg accttggtct
      721 cgacgatgcc aaagcggcag aactcgctcc ccgtgcacgc gacaaccccg cgctcgaaag
      781 caccggggtt gggcgagaac cgctccagaa gcggctccgc gagtagatcg tcgagccgct
      841 cccctggaac gcccgtgatg acgaggttct ggtccgtggc aagacggacg ccggccccgt
      901 attccctggc gagcctgccg gcctcctcga attgttcgcc acccatcctg ccgacgggca
      961 cgttcaggcc cacgtagtgg aggtcggttc cagaatcatc cctacctttt tgagggtgga
     1021 caccgacatg gtcgccgcgg tatctcctgg tcaggtcttc gccggctggc gtcaggtcta
     1081 cggagacgcg cctggctagt tcctccctga agccctcagg gcccagttcc tggaccaggt
     1141 agcgcatcct cgcggtccag cggttctccc ggttcccgag ctcgccgaac acctgggcta
     1201 tgccgcgcgt tatctccacg gcctcctcgg gccggacgaa cacgtctata tcggaggcca
     1261 tccttgggcc gtcggagagg ccgccgccga cgcgcaggtt gaagccaagc gtgccatcct
     1321 gaagacgggc tgggagcagg ccgacatcgt tgatctccgc ctgcgcgcag tcctccaggc
     1381 agcctgtgac gctcagcttg aacttgcgcg gcaggttggc gtactcgcgg ttgcccgtga
     1441 aataggagct tatggcctgg gccaccggga aagcgtcgat gacctcatcg tggtcgaggc
     1501 ccgagaccgg gcagcaaagg acgttgcggg ccgagtcgcc gcaggcctgc acggtcgtga
     1561 tgccgacctc ttccaggcgg cgccagatct ccggtatgtc acccatccgg agccagtgca
     1621 tctgcacgtc ctggcgcgtg gtcaggtcga gaaagttgtt gccgaagtgc gggttcggga
     1681 tgggtccgtg ggcaaactcc gaggcgatcc ttcctatcac ctccgtctgc gccggcgtca
     1741 gcacgccgcc tgggatcttc acccgcatca tataggcgtc gccgccctgc ttctgcgggt
     1801 agatgcccac ccacttcagc cgctcgacct cccccgggac ctcgtccatt gccgataggt
     1861 ccttggagta agtgtctatt atcgcctggc ggacctccag cgggtggcgc gcctgcttga
     1921 tgcgttcgac gttgtttagg gtctggtctt ccctgagaaa tctcatgttg taaaaacctg
     1981 ctctattaga gaaaggtgta ccttgattca ggcgcccgcc cggctgacgc ggccgtcttc
     2041 gaagtggatg ccgcactcgg tcttgtccat ctccgaccac cggcccgccc tggggtcctc
     2101 gtcgtcgtgc acgggcctgg tctgcggctc gcagccgatg ctcctgtagc cctggtgcaa
     2161 gagcgggttg acgagaacgt cgtgcgacct gacgtaatcc cacacctgcg tctccgtcca
     2221 gtgcggcatg ggcgctatct tggcggcctc gtagcgttcc tcccaggcga cgaccctggt
     2281 gccagccctc tgcggggtct gctccctccg gatgcccgtc atccaggcgt cgtagcgggt
     2341 cagggccctc tgcaggggct ctatcttgcg tacctggcag cacaggttcg gggtgcaggt
     2401 gcgcatctgc tcgccgtaac gttcgacctg ctcctcgatg gtcatcgcgg gccgcaagac
     2461 ctctacgggt agcttgtagc gccgcatgac ctcctcgcgg aactccgccg tctccttgaa
     2521 gatgaagccc gtatcgatgg taagcacggt aaccttgtcc gtgacgctgg agatcatgtc
     2581 cagaagcgcc atcccctcgc ccccgccgaa cgaggcagag agcgccaggc cctcaccgta
     2641 ggtctcgacc gcccacgcta tgacctcccg cgggtcggcg ccctccagac ggtcggactc
     2701 ccgctcaaga gcctccagac caggtaagat cctatcgtta cgcttggaac gcagggattc
     2761 atgcaacgtg ccatcctcca gtgtgatcga aaaacgagat gccaggggtt gagatcaggc
     2821 gcgtgcgcta catcgtgcgc ccgcgcgagg ggccgaagac attcgcccca tcgcccctcc
     2881 ctgcgtgccg tgcaactcca tgcgcggata ctaagggtca ggcggcaata cgtcaacaat
     2941 aattccgatg aaaattgtcg gaattagaga aaaacgctct gttgcggcaa taccggcttc
     3001 ttgcaaaggg ttatttttcg ggggtgttgt cgccgagttc gggttcggcg ccaccgcgtt
     3061 ttatggcgcg ggcgtagagc aggagggtcg gtgatagaac aagggcggcc acggtcaggg
     3121 cctgcctgac gccgaagacg ttgccgataa cccccaccac ggggccggcg gaggcttcgc
     3181 ccagtgcgcc ggcctggctg ctcatggaga tcacggtcgc cctcacggcc gggtcgaggc
     3241 cctcgttgag ccaggtcagg tagaccggct cggcgacccg gcggaccagc gtcgcgaacc
     3301 agaacgcgcc gagcgcgagc gcgaagctac ctgcaagggc gaaggtgagc attcccgcca
     3361 caagcagcaa gtcgaggacg agcaggagcc gcgcggcgac ggcggggttg ccaacatcga
     3421 tggcgcgggc cacgatgccg gcggccaggt agctgaggac gaggcccccg gcccctatcg
     3481 cgccgaacca caccaccggc tcgaggcccc cgatggtcgg cagcccgacc acatcgagga
     3541 actgcttggg gttgagccgg tcgaagccct cctcggacat gccggtaaac agggccgcgg
     3601 ccagcaggat gaggaggatg ggcctggagc gtaccagccg gacgccgccg cgagccgtgg
     3661 ccgcggcgtg ctcgactgag gagcgtccct cgcgcggaga tggacggaaa ttgcgctccg
     3721 gcatagccag gatcagggcc aggccgagcg cgacggtcag gactccaccg gcgagcagcg
     3781 gcaggttcag ggcgacgagg ccgaggagca cgccccccgg tatccctaga aacgagccaa
     3841 ggtagtcggc ctgctctccc cggatgtaca ccctccccag gtcgcggtcg ggggcctcgt
     3901 ctgcgatcca ggcctccagg gccccgctga tgaacgtgta ccccaccccc cacacggcct
     3961 gcgccgcgag cacggcggag aactcgggga tggcgccctc aagcgcgaac cccgagccca
     4021 tgagcacgca gcccacgatc accgaccgtc gccgcccgaa cgtgtcggcg aacaccccgg
     4081 tcggcacctc gaagacgaac acggccacct ccagcgccgt cccaagcagc accagccgca
     4141 gcgggtccag cccgaccacc tcgatcctgt aaaccggtgc cacggtgctc caaagcgtaa
     4201 aaaacagcgc gaaagagccc ttgataaaga ggtagatcgg atacgctcca agcctgttca
     4261 cgccggcccc gctctctttc caccccggtc agaaccgtgc cgggaagaag gatggaattc
     4321 tcactggtgg tgccccgagc cacgatggtc gtggaaacgg gaggctgctg gggtacggcg
     4381 gagcgagaac cgcgagcttt gcggtcccct ctttcgtctc cgccgcctag cgaggaatta
     4441 gcacggctcg atcgtaaccc atccccgacg acgccgtcaa gaagccgaaa aacctgtgct
     4501 acggggtctt acctgaacat gtctcgttcc ggaggcatga gaaggtcccc ggcgctaccg
     4561 gatgccggcg agtacgggta tccccgcacg atggccaggg gtataccctc ggtcttgccc
     4621 atgacgagct ctgccgcggc ggccagctcg tcggcgacgg ctagcacgct cgcctccatc
     4681 gggtagccgt gcgggtcgtt caggcctctg tagtcggcga tcgggtccat gccggcgacg
     4741 ccgatggcga cgtccgttat cccgtggcgc caggcccggc cgaaggagtc ggagatgatg
     4801 accgccacct cgacgccgag ttcagtggcg agggtcccgc ggagctttgc ggcggaggcg
     4861 tccgggtcga cgggtagcag gcagaccgta tcgtcgccag gcacgttcga ggcgtccacg
     4921 ccagcattgg cgcagacgaa gccgtggtgc gtctcggaga tcacgatgcc acggtccatg
     4981 cgcacgatgc gccggctctc cctgaagacg acctcgacct ggcgggcgtc ctttccgtgg
     5041 cgggcagcgt agtccttcgc cagcgcggag ggttcgacgg tgccgagatc ctggatccgc
     5101 ccttcggcct tgctgacgat cttgtgcgtc acaaccagca cgtcgccggc gctcaggtca
     5161 ccggccacgg cctgccggat catacggtcg aggtcgtcac cggggaggac ctcggggaag
     5221 ccctcgaccg aaagcacctg caatcctttc acgggcgcgc cacgccgttc ttcttgagca
     5281 ttgccaggtc ccccgccgtg tcaaggtcga aggccaggtg cggcctctcg cagaccctga
     5341 ccgcgagccc ccgcttgcgg gcgtcctcca ggtgcattcc gaagctgcct ggaccgaagg
     5401 cgaacggcag ggcgtcgggg gggcgtgtca ggagggcgtt cgtgccgaaa cgcgccccgt
     5461 ctggggaaat tactacggcc tggccctcgg atgcctccag cacggcgcgc acgtcttcgg
     5521 cttcgaggag cggaaggtct gcgggaaaga cgagcagggt ggatgctccc agatcaatgg
     5581 ccctgcgccg tccctcttcg agggcggggt tgagcccgcg gctctcctga acgagcggcg
     5641 ctgccccgcg tctcctggcc tcgttgagca cgatccggtc cgggctcacc acgcacacgt
     5701 cctccacgcc ggaggaccgt atagcggcca ccacgcggcc catcatgtag agggtgagcc
     5761 ctgccctggc gccggggttg agtatcgggg agagtcggct cttggtcccc tgcaaatcct
     5821 tgaccggcac gaccgccgac accccggaag tcaggctcaa cgcgccacca tgcccaaccc
     5881 gaactccagc gtctcacccg cgaggcgggc ccggtccccg tcgtcgcgca tcacggcatc
     5941 ggtcacgagc acgcgtaccc ccgacttttc tatgccacca cgttcttccc cgtcgaccga
     6001 atccacgacc ataccgtcca ccagcccaga gtacatcgcg gccacacccg tggccgagac
     6061 ctcgtggccg agcgagagga gcatcctgtc agccggcccc ttgagcgccc tccccccgac
     6121 gatcgggctg acggccacct tcggggcgcg ggatgaggcc agtgcctctc tcatgcccgg
     6181 caccgccagg atcgggccga cgctcactac cgggttcgac ggacagaaaa tgatggcctc
     6241 cgcgccggag atcgcctcga atacctcgtc ggtcggcccc gcgtcgtcta tgccgaggaa
     6301 ctcgaccccg agcacctcgt ccttctgcct gcggcgcacg aagtactcct ggaattccag
     6361 gcggcccgtg ggagtatcga ggaccgtagc gaccgggtcg tcgctcatcg gcaatacggt
     6421 gctctcgatg cccagggccc cgcggagcgc gtccgttacc tccgtcagcg tctcgcccga
     6481 gcgcaggcgg gacgtcctca acacgtgggt ggcaaggtcc ctgtcaccca gcttgaacca
     6541 ggtgtcctca ccgtagtgat ccagcatgtt cagggactcg aaggactcgt ttgcgatgcc
     6601 ccatcccgtc tcggggttgg cgatgccggc gagcgtgtac atcacggtat ccaggtcggg
     6661 acagatgtgg agtccccaga ggtcgaagtc gtcggcggtg ttcaccacca ccgagagatc
     6721 cccggccggc aacacgctct gcaggcccgc ggcgagcttg gcgcccccga ccccgccagc
     6781 cagggccacg accttcatcg cgccaccgtc atgggatcgt tctctctaga tggcggcggc
     6841 atcgctactc cccagctttt cgagcgcgta gttcgtcagg gcgaggatgc tgagggcgtc
     6901 ggagatcctg ccgtccgttg tcattgccac ggcctctcgc agggggacgc gccgcacccc
     6961 gataacctcc gtgccctcgt tctgcggctc gccggcgatg aggtcggtgg caaggaacca
     7021 cacggcgtgc tcgtcggcga cggagttgga gaggaacgcc tccccaagcc tggtccacct
     7081 tctggccacg aggccggtct cctccttgag ctcgcgcctg gctgcctcca gcggctcctc
     7141 gccttcgggg cagccacctt cggggatctc ccaggagtaa cgttccaggg ggtagcggta
     7201 ctggccaacc aggtagacgt ggtcgtcttc tacgggcaag acgcccacgg cggtgttctt
     7261 gtagtggatg acgccgtaga tacccggctc gccgtccggc cggatcacgc tgtcttcacg
     7321 gaccgagatc caggggttct cgtagacgca cctcgagccc agagtccgcc acgggttgtc
     7381 tgtcgcttcg ccctccaagg tctctccaga ctagtgactt ccggcggcga tccaccgacc
     7441 ctgcatacgg gctgctatct tagcaccgcg gtcggccgcg ctttcaggta ctctggcgcc
     7501 cccgggacca ggcgccgtgc tgtatctcgt acctgtactc gtcgctcgtg gagcccttga
     7561 accggtccgc atccttctgg aaaccgaccc tctcgaagcc aagtgactcg accagggcta
     7621 tggaggcaac gttgcgcgtg tcgatctccg ccgcgaccac cccgaccccg tagtccgcga
     7681 acaggtgctc gagcaggcgc ccgcacgcct cggcggcgaa cccctgccgc tggtagggcg
     7741 cgaagaccat gtaggcgata aaggcgggct cgcttgcttc taccgtggcc tcgagggtgc
     7801 cgacgtagtc accggaacgc ttctcgcgca ccgcccagtt gagccacgcc tcccggccgt
     7861 cgggagaacg ccgcgccgac aggaattcgt agcgattctc cagggcttgg ggtgtgttcg
     7921 ggggatcctg ggggataaac cggtacagcc gctcctcctg cattcgttcg tagagggccg
     7981 gcgcgtgggc cggcaggatc ggctccagca gcagccgccc cgtttcgagc cgcgcttccg
     8041 gtgctacgag gtccatctct tcagggggca ctctgggtct cccgttcgct gttcgtgggg
     8101 ccgaaggcta tcgcgactgc tatcgtagca ccatcgaggt cgaactcgtc ggatggccga
     8161 agggaggcga aggttgactt accccgaccg ggaattcgtg aagaggcagt accgggacgc
     8221 ctccaggctg aacgcctgca tcgggctaca cgagcgcttc agcaccaatc cacaagggct
     8281 gcaggactgg gtcttcgacc acttcgacat tccggaagag gccagcattc tggatgtcgg
     8341 gtgcggttcc ggcctgttgt ggagaaagaa cctgaaccgc gttccgcggg aatggtgtgt
     8401 cacgctaacc gacgcttcac cggggatgct agacgaagcc agggccgatc tctctgccga
     8461 tcaacgcttc gggttctgga tcgcagacat tcagcacttg ccttctgtag atgggtgttt
     8521 cgacgcagtt atcgccaacc acatgctcta ccacgtgccg gacaggccga gggctatctc
     8581 agagatctcc cgtgtcttgc gaccgggcgg aaccctgtac gctacgacga acggcagaga
     8641 gatgcacaag gagatgggct ggatgcaacg cgtcctcgat cccgcccggc cttcggacgc
     8701 ccgcttctca gaccctctcg gcttcagcct ggagaacggg gccgatcagc tctcggcatg
     8761 cttcacggac gtcaccatga agaggtacag cgacgccctc gccggcacgg aggtagaccc
     8821 gctcgtcgac tacctgctgt cagaatcgtc agccgacgcc gcggagagga gaaccgacgc
     8881 cgaggaattc gaacggaggg taggggagct ggtagagctt ctggagcacg aactggattc
     8941 acagggtgcc atacacataa ccaaagacac ggggctgttc gttgcgcgca actagggaca
     9001 tggagatcct cgtcggggca ccgctcgatg accccgaggt cggcttcggg ctgcagcagg
     9061 ccgtgctgga ggaagtcgcg gctggtgagc gtggccctgc ggccctgatc tggacttcgt
     9121 cgcgctacgt aggggccacg aggagggaga cgcgcctctc cggcttcgcg ctggcctcgg
     9181 aggcggccat ggaactcggg tttcccgtgc tggtgcgcaa ctctggcggg ggggccgtcg
     9241 cggcgaacat gggctcgctc tcgttctctc tcaccttccc cgtggaggac ctgcgtcaag
     9301 ggctctacga acgatactcg gagggcctgg agctcatcgc ctcggcactc aggcgcgtcg
     9361 gcgtcgaggc ggagcggggg gaggtcgagg gggagttctg cccgggcgcc tattccgtgc
     9421 gatctggggg tcctggaggg atcaagcacg ccggtctggc ccagcgggtt acgcgccgcg
     9481 cggcgcgcct cgaagccctg ttgctggtct cggagacgga cgaggtcagg gcggttctcg
     9541 agcgcttcta cggccttctc gggctaccgt tccgtccggg gagcgtcggg gaccttccgg
     9601 tagacgttcc gacggtgatc cgggcgctct cggacgaggc tcgtgccagg tacggggcca
     9661 ggggggtcgg gatcggggag gagactttgc agcgggcgcg ggcgttgcgg aacgggtggc
     9721 gctttatccc tgattcgggt agctctctgt agggtaaagt ttttgcatca tggagtacgt
     9781 gttcaacgct tttgtgaccc ttctggtcat catcgacccg cttggcctcg cccccatctt
     9841 cgccgcgctc acgcggggct tctccgaaaa gcagcggcga gagtcggcga tcaggggcac
     9901 cctgctcggt gggctcatcc tctacgtgtt cgcgctcgcc ggcgacgtgc tactcgaggc
     9961 gttggggatc gggcttccgg cgttccggat tgcgggcggg atcctgctct tccttctctc
    10021 cctggacatg gtcttcgcca ggccctctgg gatgcgcgcc agcgccgtgc gcgagcagga
    10081 ggaggaagag taccagcagg acatctctgt ctttcccctg gccataccgc tcctcgccgg
    10141 tcccggcggc attaccaccg tgctactgtt caccggtggc gcaagcgtcg gccagcaagc
    10201 cctcttcatg accgtgctct tcgtggtcct ggtcatgacc ctggcctgtc tgctgctcgc
    10261 cccccgcgtc atgcggcttc tgggcgagac cggttcgaac gtgctcacgc gcgtcctcgg
    10321 cgtcctgctc gccgccctct ccgtgcagtt cgtgatcgac ggcctacaga caagccttgc
    10381 atagaacggt cagctttcag cctccggcta tcagcactcg ccggctgacc gcggagacct
    10441 gatggctcgg aacccggcga atccgttttc gttgataccg cttatactct tcgtcatgtc
    10501 gaacgcgccc atagtgatcg tcgggggagc cacctggtgg ccgaacagtt atcgtgatct
    10561 cgcgaacacg ctacgcgaca tctccggctc cgaggttcac gtggcaccca tcacgcccct
    10621 tgactgggcg ctcgggtact tgaagggctt cgggcaactg gtgttcgagg tcgcgagcac
    10681 cgtggacagg gcgctccttg aggcagagac caagaaagcc gttctcgtgg ggcactcggc
    10741 gggcgggcta gcctgcaggg tgtatatcgg gggagatccc ccttatggtg gccggcgcta
    10801 ctccgggcac aggcgggtgg agcgcttgat ctcgcttggc accccacaca acgtcgggca
    10861 gacgaaacgc ctcgccccaa tctcccggat aaacgacctc ttccccggcg ccctgcacgc
    10921 ggaggacggc ctccgctacc tctcagttgc ggggaacgtc gttgacggcg ccacctccgc
    10981 gaaggcccgc aagcgctacg agcggttcgt cgaggacggg agggtaggcg gggatggtgt
    11041 cgtgccaatc gaggccgccc tgctacccgg ctcggaagag gtcgtcctcg atgggatcca
    11101 ccacaacagg cggatgggct cctggtacgg gtcggatgcg gagacggtcg gccgctggtg
    11161 gcccgaagcg ctacgcacgg atggcggtcc acgaacagtt cgagccgccg aaaggagcaa
    11221 caatgccggc tgacggcgcc gacgagcgac aatattcctg ggaggtcgcc ggcgctgacg
    11281 ccgacgcctt gcggagcgcg atgagccact ttccgaccgg ggttaccgtg gttaccagcg
    11341 ggcaggagga gaacgtcgag ggtatgacgg ccaacgcggt gatgtcggtc tcactcgacc
    11401 cggtgctctt cctcatcagc gtgcacaaag atgctcgcat caacccgcgc atcagggagg
    11461 aggggtactt cgcgatcagc ctcctcgccg acgatcagga ggggctgagc aggctcttcg
    11521 cctcaccaga gcggtccagc ggcctaccgg cgcttcactc gctgggcggt ggctacggct
    11581 cgaccggggc acccctggcg gctggcgccc tggcggcgat cgaatgcgag ctcgaggaca
    11641 cctactacgg gggcgaccac gacctgttcc ttggcagggt cgtcggggtg cgcctgggag
    11701 atacacgcaa gggacctctg ctcttccacg agggtgccta ccccacgcta aaatcgcctt
    11761 cccgccccga ggactccggc gccttcgtcg actccgtcag gggcttcgac gcccgcatga
    11821 accgcctgcg cgacaggaga agacgctagg tgttcgaccg gaatctgctc ccgatcgtca
    11881 ggcctcttcc cgttgacgcg gagggcctcg tcttcgtcga aggcacgacg aaagacggca
    11941 tgatcgagag tggagaggcc cgctccccga cgacgggcga gacctatgag atcaggaacg
    12001 gatacctgga cctccttgga tcccgtatag gggcggacag catcgccaac ctgaccaact
    12061 tccttccggg cgcgggccgc ggctacgaac cgctctggag ggtccgctcc ctgaggttgc
    12121 ttaccggaga gccgttcccg aacgagcagg agcttcggat catcgaagac ctggttcacc
    12181 aggctccgga cggtcgttat ctcgatctgg gttgctcagc cggcctctac acgcgcgggc
    12241 ttgcccggga ccggaatacg agcgtggtcg gcatagatat ctctccttcc atgctaagag
    12301 aggcagaccg tcgcgcccgc cagaccgaaa ccagaccctc cttcctccgc gccaacgccc
    12361 agaacctacc gttcttcgac ggcgtcttct ccggggcagt ctgcggcggc tcgctgaacg
    12421 agttcaagaa cccggcccgc gtgctacggg agacccaccg cgttctccag agcgggggcc
    12481 ggctcgccat aatgggtatc ctgcgcgcgc aaacgcggcg cggacgccgc ctccagcaac
    12541 tcgcctcgac gggcggcata aggttcttcg acccggacga gttggggagc ctcctggatc
    12601 acgcaggctt cgagcctgac ccactcagga ccaacggccc cgtcttctta gccggcgcaa
    12661 cccgcagggg ttgatcttcg gcagaagccg ccgctacaag gcttccacag attctggagt
    12721 cggcagggcg ccctgggcgc cttctctggt taccgccgcg gccccggcgc gcgcggcgta
    12781 ggcgacggcg tcttccagag aggcgtcgcg ggcgagtctg ctcgccaggg cgccgacgaa
    12841 ggcgtcaccc gccccggtgg tatcgacgac ctcgacctcc gggctagtaa cgtggcccgt
    12901 agagtctccg tcgctgaaaa ccgcaccttc tcgcccgagc gtgatcaccg ctgacttcgg
    12961 ccccagttcg agaagtgcag g
//
LOCUS       DBNGVD010000482         7305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2671070, whole genome shotgun sequence.
ACCESSION   DBNGVD010000482 DBNGVD010000000
VERSION     DBNGVD010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2671070"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="AC16CM_12990"
     CDS             <1..139
                     /locus_tag="AC16CM_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XshC-Cox1 family protein"
                     /protein_id="HIR3140795.1"
                     /translation="EETAVAIAAEIIALRTGHSGGRLAERSGAIHAPEEEGATARNPS
                     L"
     gene            complement(374..1267)
                     /locus_tag="AC16CM_12995"
     CDS             complement(374..1267)
                     /locus_tag="AC16CM_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3140796.1"
                     /translation="MQLTTERKVLLALITVVASLLLATVGAGVAWASNFDCTINAVAL
                     CEGTGNDDEFEGEDIDDGVQGRKDVIKGKGGSDGADGNGGNDRILGGGGADGDFAVND
                     ALEGGDGSDFVDGGDGADNRIQGLAGNDTVLGGRGSDEEVFGDFGKDKVSGGPGNDEE
                     VRGGDNNDTVNGGPGNDDEVSGGDGDDTVLGEAGNDANVEGGSGDDKVSGGSGDDGNG
                     PGVFGELTGDTGNNTVDGGLGDDKIDARASLAGEVENIFGGPDNDTIRADDGEVDNID
                     CGSGNDTVTFDAGVDVVDPNC"
     gene            complement(1412..1690)
                     /locus_tag="AC16CM_13000"
     CDS             complement(1412..1690)
                     /locus_tag="AC16CM_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140797.1"
                     /translation="MRKTERISETADSRSTGAATHVAPPTVGAGAPLIRQLIRLVRPR
                     PPNAVAGEDVNDATPPRGRGTDHASDAVRSPMDAGITRGASLWFGARR"
     gene            complement(1731..2336)
                     /locus_tag="AC16CM_13005"
     CDS             complement(1731..2336)
                     /locus_tag="AC16CM_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140798.1"
                     /translation="MSQTKKVIARILWLGRGTATAMGVAVMLAVVLGMATTALGAVPG
                     DPFKLGRTNTIDQMSTLLGSTSGTLLRINNKGGGPALDLRVEEGKAPMNVNSNARVNG
                     LNADRLDGKDSRAFVPTESYTVSELLEGEGGGDIEGTEAKCDRGDTLLGGGFDASADD
                     TVISSEPLVSGIGWSVAVKDNGISTVTFVTALCADLPPLKP"
     gene            complement(2374..3219)
                     /locus_tag="AC16CM_13010"
     CDS             complement(2374..3219)
                     /locus_tag="AC16CM_13010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3140799.1"
                     /translation="MTVEDHRKEDLTYWLGRDDAETRRLIQQSRFLNPFTGFLLREAG
                     ISEGMRVLDVGSGAGDVALLAADLVGPGGSVVGVDTNPEILETARARASLAGLTNTEF
                     VAGECRSADLGDGFDAIVGRLVLLYVADPTETLRSLAERLEPEGIVAFQDYNFTPNSV
                     QSFPPMRIWQEVYDWVRAAARQAGLNMAMGYDLRRIFIEAGLPEPNMQIASPAGGGPD
                     WEGYENIAATIRSMLPLVLASGVATAEKVGIDTLADRLRTETVANGGVVKFPDLVGAW
                     TRKAN"
     gene            complement(3383..5683)
                     /locus_tag="AC16CM_13015"
     CDS             complement(3383..5683)
                     /locus_tag="AC16CM_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140800.1"
                     /translation="MRGKLPLELSSFVGRERELSEVERLLAGTRLLSLTGTGGCGKTR
                     LALKVAGNLVGKFDDGVWWVGLAPLSDPALAPKSVASALGVRERPGRQMMETLSDHLR
                     SRKLLLILDNCEHLIGACATLAEALLRTCPDLKILATTREPLGIAGEANWPVPSLSVP
                     AAHPLPPVHELVRYEAVGLFVERAAEAGPGFEPTEHNAPAVVRVCRKLEGVPLAIELA
                     AARTKVLTVEQIADRLGDSFGLLTEGSRTALPHQRTLRATVDWSHGLLSEGERDLFQR
                     LSVFAGGFTLEAAEEVCSGRGIEQVEILPLLSRLVDKSLVVTEVREEHVRYRLLEAIR
                     QYGEEKLRESGGASSNRQRHAGYFLALAEEAEAEMSGPDQAACVERIQREHDNLRAAL
                     GWLEAAGDAERGLRLAAALLRFWWFRGHIAEGRARLEVLLGIPGKPVEDGVRARALYT
                     LGVLTYRHADDAAGDWDTARSRLEEGLEIYRGLGDEAGTAATLQELGRVLAELGEWTR
                     AHSLLDESLEIGRRLDDEIAIAFSLFTLGRVYWLRDQRPSARALFDESFVVFQKLEDR
                     FWINACLVFLGYIDCEEGDYASALSRFAKMNETVPVIRFPWGATYMLDGYARLAAAQG
                     QASRSLRLGGATDMLRQVYGVSIGPRSENHFQRSLRPAWEALGKKEGMAAWEEGRATS
                     LEEAVALALQEPGASEGSPSGTVLSAREAEVLSLVAGGLTDVQVAERLYLSRHTVGHH
                     LSSVYRKLGVRGRTAAAHKAGELGLI"
     gene            6052..6744
                     /locus_tag="AC16CM_13020"
     CDS             6052..6744
                     /locus_tag="AC16CM_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3140801.1"
                     /translation="MSQRPTRVAPDTTLPTGGALRSRVSAILLAAGAGSRFGGDKLLA
                     SYKGRPLVETVLSQLHEAPVKEIIAVVGDLTGEGGVRLRQVCLLYGAIIAENPRWEEG
                     ISSSVKRGLAACSPDTDAAVIVLGDQPLVGAVAVGRLLEAHEGGASVAVATYGGRRRN
                     PALFARRTWPMLGEVLSGDEGARGFLERHPEMVTEVACDDVADPADVDTREDLRRLEA
                     RGLRGPVKPEGG"
     gene            6748..7185
                     /locus_tag="AC16CM_13025"
     CDS             6748..7185
                     /locus_tag="AC16CM_13025"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3140802.1"
                     /translation="MKLENEFTVNTSMDEAWKALNDLERVTPCLPGAQLTAEVGDEYE
                     GTMTVKMGPVTQKYNGTVKIEETDDSAHRAVIRANGKDARGQGTASATITSTLSEGDG
                     GVHVLVETDMHLTGRVAQFGRGMHKEVASKIMGRFAECLEKEL"
BASE COUNT         1199 a         2471 c         2322 g         1313 t
ORIGIN      
        1 cgaggagacg gcggtggcca tcgccgcgga gatcatcgcc ctgagaaccg gacactcggg
       61 gggacggctc gccgagcgtt cgggggcgat acatgcccct gaagaagagg gggcgacggc
      121 ccgcaaccct tccctgtagg cggggccgtc aggttcgcct accgatggtc gctctcgtga
      181 gggctatatg tagcccgggt acatcagacg cttactagag cgtctccggg ttccgctcag
      241 agaaaaatcc ccacgaagcc ggggtgtggt ggctgatcta aggcgggccg gggcgcaatg
      301 gcccctgccc gtttgtaacc cacgatgagt aagttgtagt tctcccggtt tccttcggta
      361 ccgtgaaccg gttctagcag ttggggtcga cgacgtctac gccggcatcg aaggtcaccg
      421 tgtcgttgcc ggagccgcag tctatgttgt ccacttcgcc gtcatccgcg cggatcgtgt
      481 cgttgtcggg accgccgaag atgttctcca cttcccctgc gaggctggct ctggcgtcta
      541 tcttgtcgtc gccgagtccc ccgtcgaccg tgttgttgcc ggtatctccc gtaagctcac
      601 cgaagacgcc gggcccgttg ccgtcatcgc ccgatccgcc gctgacctta tcgtctccgc
      661 tgccgccctc cacgttggcg tcgttaccgg cctcgcccag tacggtgtcg tcgccgtccc
      721 caccggagac ctcgtcgtcg ttgccgggtc cgccgttgac ggtgtcgtta ttgtcaccgc
      781 ctcgtacctc ctcgtcgttg cctggcccgc cgctgacctt gtccttgccg aagtcgccga
      841 acacttcctc atcgctaccg cggccgccca ggacggtgtc attgccagcg agaccctgta
      901 tcctgttgtc ggcgccatcg ccgccgtcta caaaatcact gccgtcgccg ccttccaggg
      961 catcgttgac ggcgaagtcg ccgtccgcgc cacccccgcc gaggatcctg tcgttgccac
     1021 cgttcccgtc ggcgccgtcg ctaccaccct tgcccttgat aacgtccttg cggccctgaa
     1081 ctccgtcgtc tatgtcctcg ccttcgaact cgtcgtcgtt gccagtgccc tcgcaaaggg
     1141 ccacggcgtt gatggtgcag tcaaagttgg atgcccaggc taccccggcg cccacggtag
     1201 ccagcagcaa actcgccacc accgtaatca gcgccagcag aaccttgcgc tccgttgtca
     1261 gttgcatcgt tcgtatctcc tttgtagttt gcgttggctc tttaactacg cctcagtacc
     1321 tcatcggttc acccctttcc ttctaggcga gatcctcggc acacgctgcg taagggctgc
     1381 cgcgcctccc tgaggttttc cggtaggaac gtcatcttcg ggcgccgaac cacagcgatg
     1441 ctcctctggt gatgccggcg tccatcgggg atcgcaccgc gtccgaggca tggtccgtcc
     1501 ctcttccgcg gggaggggtt gcatcgttca cgtcctcccc ggccacggca ttgggcggcc
     1561 ggggccgcac gagccggatc agttgccgga taagtggggc tcctgcgccg accgtgggtg
     1621 gagctacgtg cgtggccgcg ccagtggaac ggctgtcggc ggtttcgctg atcctttcgg
     1681 tcttcctcat ctctgttctc ctcgcttcag cggtccttgt attggatgca ctacggcttc
     1741 agcggcggaa ggtccgcgca gagggccgtc acaaaggtca cggtgctgat gccgttgtcc
     1801 ttgaccgcca ccgaccatcc gataccggag acaagcggtt cgctggatat cacggtgtcg
     1861 tcggcgctgg catcaaatcc tccgcccaaa agcgtgtctc ctcgatcgca tttggcctcg
     1921 gtaccctcta tatcgcctcc gccctcaccc tccaggagtt ccgacaccgt ataggactcg
     1981 gtaggcacga aagccctcga gtccttgccg tccaggcggt ccgcgttcag gccgttgacc
     2041 ctggcgttgg agttgacgtt catgggggcc ttgccctcct cgacccgcag gtcgagggcc
     2101 ggtccgcctc ccttgttgtt gatcctgagc aaggtgcccg aggtgctgcc gagcaacgtg
     2161 ctcatctggt ctatggtgtt ggtcctgccc agcttgaagg gatctcccgg taccgcgccc
     2221 agggctgtgg tcgccatgcc aagcaccacg gcgagcatga ccgccacgcc catcgctgtg
     2281 gccgtcccac ggcccagcca caagatcctc gcgataacct tctttgtctg gctcatctgt
     2341 cctcgttcct ctccgttcgt gatggccgtg ccgtcagttg gccttgcggg tccaggcgcc
     2401 cacgaggtcc ggaaacttga ccacgccacc gttcgcgacc gtttcggtgc gtaggcggtc
     2461 ggcgagcgtg tcgatgccga ctttctccgc ggtagcgacg ccggaggcca ggacgagcgg
     2521 gagcatgctt ctgatggtgg ccgcgatgtt ctcgtaaccc tcccagtccg ggccgccgcc
     2581 ggccggagag gctatctgca tgttcggctc gggaaggccg gcctcgataa agatacggcg
     2641 caggtcgtag cccatggcca tgttcagccc ggcctgccgg gccgccgcgc gcacccagtc
     2701 gtagacctcc tgccagatcc gcatcggcgg aaaggactgg acggagttcg gcgtgaagtt
     2761 gtagtcctgg aatgccacga taccctcggg ctcgagacgc tctgctagcg aacgcaacgt
     2821 ctccgtcgga tccgccacgt agagcagtac cagcctgccg acgatcgcgt cgaacccgtc
     2881 tccgaggtct gctgatcggc actcgccggc cacgaactcc gtgttggtca ggccggcgag
     2941 gcttgcacgg gctcgtgccg tttccaggat ctccgggttc gtatccacgc ccacgacgct
     3001 accgcctggg ccgacgaggt ccgccgccag caaggcgacg tcgcccgccc ccgaaccgac
     3061 gtcgagcacc ctcatcccct ccgagatgcc ggcctccctc aggagaaagc cggtaaaagg
     3121 gttcaggaaa cgggactgct ggatcagacg ccgtgtctcg gcgtcgtcgc gtcccagcca
     3181 gtaggtgagg tcctctttcc tgtgatcttc cacggtcatc gctcgatcca cctccgcagg
     3241 gttgctgttc gctgccaggg gcacgttaag ctgcgggcgg acggagggaa tcgggaggat
     3301 tactgattca gatgagggga gccggaatag cgaaaactac ccagtacgaa ttacgtcggg
     3361 ctacgaagcc gtcttcggcg gcctagatga ggcccagctc tccggccttg tgggccgccg
     3421 cggttcgtcc tcgcaccccg agcttgcggt agacggagct gaggtggtgt cccacggtgt
     3481 gacggctcag gtagaggcgc tccgcgacct gcacgtcggt caggcccccg gccaccagag
     3541 agagaacctc tgcctcgcgg gcactgagaa cggtcccgga gggagagccc tctgacgcgc
     3601 ccggctcttg cagtgccagg gcaacggcct cctccaggga ggtcgcccgg ccctcttccc
     3661 aggccgccat accctccttc ttgcccaggg cctcccaggc cggcctcagg ctgcgctgaa
     3721 aatggttctc cgagcggggg ccaatcgaca cgccgtagac ctggcgcagc atgtccgtcg
     3781 cacctcccag cctgagggat cgcgacgcct gcccctgggc cgcggccagg cgcgcgtagc
     3841 cgtccagcat gtacgtggcg ccccagggga atcggatcac gggcaccgtc tcgttcatct
     3901 tcgcgaaccg ggagagggcc gaggcgtagt cgccctcctc gcagtcgatg taacccagaa
     3961 aaaccaggca ggcgttgatc cagaacctgt cttcaagctt ctggaaaact acgaagcttt
     4021 cgtcgaacag ggcgcgtgcc gaggggcgtt ggtctcgaag ccagtacacc ctgcccaggg
     4081 taaagagaga gaaggcgatc gcgatttcgt cgtcaagccg ccgtccgatc tccaggctct
     4141 cgtccagaag ggaatgtgcc ctcgtccact cgccgagctc ggccaggacg cgtccgagct
     4201 cctggagcgt ggcggccgtg cccgcctcgt ctcccaaccc gcggtagatc tcgagcccct
     4261 cctccaggcg cgagcgggcc gtgtcccagt ctccagcggc gtcgtcggcg tgccggtagg
     4321 tcagcacccc aagggtgtag agtgccctgg ccctcacccc gtcctcgacc ggtttccctg
     4381 gtatgccgag caggacctcg agccgggctc taccttcggc gatatgcccc cgaaaccacc
     4441 agaagcggag cagcgcagcc gcgaggcgca agccacgctc ggcatctccc gctgcttcga
     4501 gccagcccag ggccgccctc aggttgtcgt gctctcgttg tatccgttct acgcaagccg
     4561 cctggtccgg ccccgacatc tccgcctcgg cctcctctgc cagggcgaga aagtagcccg
     4621 cgtggcgttg acggttcgag gatgcccccc ccgattctcg tagcttctcc tcaccgtatt
     4681 gccggatagc ctcgagcaga cggtacctta cgtgctcctc ccgcacttcc gtgaccacca
     4741 gggatttgtc caccagccgc gagagcaggg ggaggatctc tacctgttcg atgcctcgtc
     4801 cggagcaaac ctcctcggcg gcctcaagcg tgaacccgcc ggcgaagacc gagaggcgct
     4861 ggaacaggtc acgctccccc tccgaaagca gcccgtggct ccagtctacc gtggcgcgca
     4921 acgttctctg gtggggcaac gccgtcctgc tcccctccgt caacagcccg aaagagtcgc
     4981 ccaggcgatc ggctatctgc tctaccgtaa gcaccttcgt tctcgccgcc gcaagctcta
     5041 tggccagcgg tacaccctcc agttttcggc acaccctcac gacggccggc gcgttgtgct
     5101 ccgttggctc aaaaccagga ccagcctccg ccgcccgctc gacgaagagg ccaaccgcct
     5161 cgtagcgcac gagctcatgg accgggggca gaggatgggc ggctggcacc gacagggaag
     5221 gcacgggcca gttggcctcc ccggcgatgc caagcggctc acgggtggtc gccaggatct
     5281 tcaggtcagg gcaggtgcgc agcagggctt ctgcgagcgt cgcgcaggcc ccgatcaggt
     5341 gctcgcagtt gtccaggatc agtagcaact tccgggacct caggtggtcg gagagcgtct
     5401 ccatcatctg tcggcccggt cgttcgcgca cgccgagggc cgaggctacg gatttcggcg
     5461 cgagggcggg gtccgagagg ggcgccagcc caacccacca gaccccgtca tcgaacttcc
     5521 cgacgaggtt gccggcgacc ttcagggcga ggcgcgtctt gccgcatccc ccggttccgg
     5581 tgagggagag gaggcgggtg cccgcgagca gcctttcgac ctcggagagc tcacgctccc
     5641 tgccaacgaa gctggagagt tccaggggca atttgccccg catcgcacgt gtagacgagg
     5701 gaccggccgg ttcctcatcc ggcccgccgg tgccccccgc gccggcaacc ccgagcggcc
     5761 tcacctttga tgggcgtcct ctagccaagt cgctctctca tcttcttgta gccaccgtaa
     5821 cagcaatccg caagtgctgc atctcacggc gccagatctg ccggcgggac ggggagacgc
     5881 caggccgacc acccagccgg ggtaggatcc ggccccgcac gtctttgcta tactgccgcc
     5941 cgcccagatt cgcaccatct ggtggggtct gatggcggca tggcccggga aaggagtagg
     6001 cgttgcatga cgaagcccgt ctagctctcc cggagaaact cgtctacgtt cgtgagccag
     6061 agaccgaccc gcgttgcgcc cgacacgacc ttgcccaccg ggggagcgtt gcgtagccgg
     6121 gtctccgcca tcttgctcgc cgccggggcc ggcagccggt tcgggggtga caagctcctc
     6181 gcctcctaca agggacgtcc gctcgttgag acggtactgt cccaactgca cgaggccccc
     6241 gtgaaggaga taattgcggt ggttggggac ctgaccgggg aagggggggt aaggctgcgt
     6301 caagtgtgtt tgctctacgg ggccatcatc gccgaaaacc cgcggtggga ggagggtatc
     6361 tcctcctctg tgaagagggg cctggccgcc tgctcgcccg atacggatgc cgcggtcatc
     6421 gtgctcggag accagccgct ggtcggggcc gtggccgtgg ggagactact ggaagcccac
     6481 gaggggggcg catccgtggc cgtggcgacc tacggtggca ggcggcgcaa ccccgcactc
     6541 ttcgcccgca ggacctggcc gatgctagga gaagtgctgt cgggagacga gggggcgagg
     6601 gggtttctgg agagacatcc cgagatggta acggaggtgg cctgcgatga tgtagcagac
     6661 cccgccgacg tggatacccg ggaagacctg cggcgtctag aggcgcgtgg tctcagaggg
     6721 ccagtgaaac cagaaggagg ctagacgatg aagttggaga atgagttcac ggtcaatacg
     6781 tccatggacg aagcctggaa ggccttgaac gacctggagc gggtgacccc gtgtttaccc
     6841 ggggcccagc tcacggccga ggtcggcgac gagtacgagg gcaccatgac cgtcaagatg
     6901 ggccccgtaa cccagaagta caacggcacc gtcaagatcg aggagaccga cgacagcgcg
     6961 caccgggccg tgatccgggc caacggcaag gacgcccgcg gacagggaac ggcctctgcc
     7021 accataacct ccaccctgag cgaaggggac ggcggcgtgc acgtgctcgt cgagaccgac
     7081 atgcacctca cgggaagggt cgcccagttc ggacgtggca tgcacaagga ggtggccagc
     7141 aagataatgg gccgcttcgc cgagtgcctg gagaaggagc tctagagggg agaacaacgt
     7201 gaagatggag aacgagttca cggtggaagc ccccgtcgag caggcctggg agaccctgct
     7261 cgacctggag aggatcacac cgtgcctccc cggcgcagcg ctgga
//
LOCUS       DBNGVD010000483         3672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3216346, whole genome shotgun sequence.
ACCESSION   DBNGVD010000483 DBNGVD010000000
VERSION     DBNGVD010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3216346"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC16CM_13030"
     CDS             complement(<1..350)
                     /locus_tag="AC16CM_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140803.1"
                     /translation="MATSTSQADILVLSSAIGSGHMRASAALAQGVGLVDPDRTCSIV
                     DFPHEVSPAVEDLIRRTYLESLKLMPDLYGKLYRMSETRATHQETPSRATELYERWQQ
                     FTEQWAAGQDPAQN"
     gene            514..903
                     /locus_tag="AC16CM_13035"
     CDS             514..903
                     /locus_tag="AC16CM_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3140804.1"
                     /translation="MAGGTLDQEFVEDQRRRLVDLREELTGLRDGLEEDEVELEGGGD
                     DFSETDSGDMSQSLFDREMDASVGEGIERRLGEVERALQKIEEGTYGVCEDTGEEIPR
                     GRLEAVPEAVRTVEAQQRFERERRPPA"
     gene            988..1854
                     /locus_tag="AC16CM_13040"
     CDS             988..1854
                     /locus_tag="AC16CM_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3140805.1"
                     /translation="MDYRSDSEQAHEPIFRSDVEGPGPQQSYEPPKPRGIWGVLKRFL
                     VPLAALGVLLFKFKGLALLLLKVKFLGTALTMLLSIGVYALLFPIWVAVGIVVLIWVH
                     EMGHVLQLRREGIPASAPMFIPLLGAFVAMKQMPKDALAEARVGLAGPVLGMLGGFAT
                     LGLYAATREPVFLGLAYFNSIINLFNLAPLLPLDGGRAVGAMSLAFQVAGLVVMVALF
                     FVAPIMAFIALLGLPELWNRWKNRNTSEGRAYYDIPFRNRIAVGAVYIGLIVVLGLMT
                     GATFVADPGSLL"
     gene            1858..3027
                     /locus_tag="AC16CM_13045"
     CDS             1858..3027
                     /locus_tag="AC16CM_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3140806.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALKASRAGKKVLVMSTDPAHSLA
                     DAFDTEIGPEPREMATGLWAQEMDHTSMIEDNWSEIQGYMTTLFEWQGTDTLAAEELA
                     MLPGMDELFGLLKVRQHNQEGLYDALILDAAPTGETLKLLSLPDHMSWYVEKIFPLQR
                     RAAKLVRPFANRARSLPPLPEDSVFAAGQRFYEAIASVEEILTDRDDASVRLVVNAEK
                     MVIAEARRAYTYLNLYDYGVDAVVINRLLPDSITDPYFAGWREAQTRHIKTIEESFAP
                     IPILTARLYEREMFGLEALGALAEDVFEEAEPLDVLFKGATHDIIKSDGGYDVVFQLP
                     LAEKRTVDLSKKGAELLVKVGGYRRNILLPDALARLSASGASFEEGALRVRLRDG"
     gene            3020..3223
                     /locus_tag="AC16CM_13050"
     CDS             3020..3223
                     /locus_tag="AC16CM_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140807.1"
                     /translation="MAEKRQRRGSMLGVVAYVLVPGERRTRAARHFRKAGFETLKGLG
                     ELVRPQGPQDPAGLASRERIEIE"
     gene            3223..3423
                     /locus_tag="AC16CM_13055"
     CDS             3223..3423
                     /locus_tag="AC16CM_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140808.1"
                     /translation="MVRLRRRDDQGGDGAGRRAIQKVSELARSEFTLAFPAAKRAATH
                     AAQAFLKKFSEEYARERKKTRK"
     gene            3554..>3672
                     /locus_tag="AC16CM_13060"
     CDS             3554..>3672
                     /locus_tag="AC16CM_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR3140809.1"
                     /translation="MAENGQSTGTFRVKSGMAQMLKGGVIMDVVDAEQARIAE"
BASE COUNT          700 a         1076 c         1248 g          648 t
ORIGIN      
        1 tcgttttggg ctgggtcttg cccggcggcc cactgctctg tgaactgctg ccaacgctcg
       61 tatagctcgg ttgcacggct cggtgtctcc tgatgggtcg cccgcgtctc cgacatgcgg
      121 tagagcttgc cgtagaggtc aggcatcagc ttcaaagatt ccaggtaggt gcgccggatc
      181 aggtcctcga ccgccggcga gacctcgtgt gggaagtcca cgatggagca cgttctgtcg
      241 gggtcgacca gccctacgcc ctgggccagc gcggcgctcg cccgcatgtg accgctgcct
      301 atcgcgctgg agagtacgag gatgtccgcc tgtgaagtag aagtagccat agcaaaatca
      361 taacggagtc ggctctccga ggctagggtg gactcctcat gtgcgcaaaa tccgttctgc
      421 agaggggatc agggcaggag agttgggaat cccgcctctg gcgtgtaagg taacggtgtc
      481 ttgtcgaaca tgtgatcaaa cccgggaggg atcgtggctg gcggcacctt ggaccaggaa
      541 ttcgtcgaag atcagagacg caggctcgtc gacctgaggg aggagcttac agggctgcgt
      601 gatggcctgg aggaggacga ggtagagctg gaaggcggtg gcgacgactt ctccgagacc
      661 gactccggcg acatgagcca gtctctcttc gaccgtgaga tggacgcctc ggtaggggag
      721 gggatcgagc gccgcctcgg cgaggtggag cgggccctgc agaagataga agagggaacc
      781 tacggcgtct gcgaggacac gggggaggag atacccaggg gccgcctgga ggctgtcccc
      841 gaggccgtaa ggaccgtaga ggcccagcag cgcttcgaaa gggagcgtcg cccaccggcg
      901 taaaataccc catgaaaccg tagggcggtt cacgaaccgc cagagaccgt ccggtctttc
      961 ctggctgacg ggctgtataa tcgggccatg gattaccggt cggattccga gcaggcgcac
     1021 gagcccatct tccgcagcga cgtcgagggt ccagggccgc aacagtctta cgagcctccg
     1081 aagccgcggg gcatctgggg cgtgctgaag cgttttctgg tgccccttgc cgcgctcggg
     1141 gtgctcctct tcaagttcaa ggggctcgcg ctgctcctgc tcaaggtcaa gttcctgggc
     1201 acggcgctga ccatgttgct gagcataggg gtgtacgccc tgttgtttcc aatatgggtt
     1261 gcggtcggga tcgtggtctt gatctgggta catgagatgg gacacgtctt gcagttgcga
     1321 cgcgagggca tcccggcctc agcccccatg ttcatccctt tgcttggggc tttcgtggcg
     1381 atgaagcaga tgcccaaaga cgctctggcc gaggctcgcg tcggcctcgc cggtcccgtg
     1441 ctcggcatgc tcgggggttt cgcgaccctc ggactatacg ccgccacgcg agagccggtg
     1501 tttctggggc tcgcttactt caactcgatc atcaacctct tcaacctggc accgctcttg
     1561 ccgctcgacg gcggcagggc ggtgggggcc atgtcgctgg ccttccaggt ggccggcctc
     1621 gtggtcatgg tcgcgctgtt cttcgtagcg ccgatcatgg cgttcatcgc gctactcggt
     1681 ttgcccgagc tgtggaacag gtggaagaac cgcaacacct cggagggacg ggcctactac
     1741 gacataccgt ttcgcaaccg gattgcggtg ggggccgtgt acatcggcct gatcgtggtc
     1801 ctgggcctca tgacgggtgc gacattcgtg gccgacccgg gatccctcct gtaagccgtg
     1861 cgtacgatcc tctataccgg aaaaggtggt gtcggcaaga cgagcgtggc cgccgctacg
     1921 gccctcaaag cgtcgcgcgc cggcaagaag gtgctcgtga tgagcacgga cccggcccac
     1981 tcgctggccg atgccttcga cacggagata ggcccggagc ccagagagat ggcaacgggc
     2041 ttgtgggcgc aggagatgga ccacacctcc atgatcgagg acaactggtc cgagatccag
     2101 ggctacatga cgaccctctt cgagtggcag ggcacagaca cgctggccgc cgaagagctg
     2161 gcgatgttgc ccgggatgga cgagctcttc gggctactga aggtcaggca gcacaaccag
     2221 gagggtctct acgacgccct tatcctcgac gccgcgccga cgggcgagac gctgaagctt
     2281 ctgagcctcc ccgaccacat gagctggtac gtggagaaga tattcccgtt gcaacgccgc
     2341 gccgcgaagc tggtcaggcc cttcgcgaat cgggcgagat ccctgccgcc actccccgag
     2401 gacagcgtct tcgccgccgg ccagaggttc tacgaggcga tagcgagcgt ggaggagatc
     2461 ctgaccgacc gcgatgacgc ctccgtgcgc ctggtcgtga acgccgagaa gatggtaatc
     2521 gccgaagcgc gccgcgcgta cacctacctg aacctctacg actatggtgt cgacgccgta
     2581 gtgataaacc gcctgctgcc ggattcgatc accgacccat acttcgcggg ctggcgcgaa
     2641 gcccagacaa ggcacataaa gaccatcgaa gagtcgttcg cgccaattcc gattctgacc
     2701 gcccgcctct acgaacgcga gatgttcggc ctcgaagcgc tcggcgccct ggccgaggac
     2761 gtattcgagg aggcggaacc cctcgacgtg ttattcaagg gcgccaccca cgacattatc
     2821 aagagcgacg gcggctacga cgtcgtgttc cagctcccgc tcgcggagaa gcgaacggtg
     2881 gacctctcga agaagggggc cgagttgctg gtcaaggttg gcggctaccg gcgcaacata
     2941 cttctcccgg atgctttggc gcggctgtcg gcgtctgggg cgagtttcga ggaaggggcg
     3001 ctgagggtga ggttgaggga tggctgagaa acggcagcgg aggggctcca tgctaggggt
     3061 cgtcgcctac gtgctcgtac ctggtgagcg acggacgagg gccgccaggc acttcagaaa
     3121 ggcgggtttc gagacgctca aaggtctcgg agagctggtg aggccgcaag gaccacagga
     3181 cccggcaggc ctcgcgagcc gcgagcgcat agagatagag taatggtccg cctcagacgc
     3241 cgcgatgatc agggaggaga cggggccgga aggcgcgcca tccagaaggt cagcgagctc
     3301 gcccgcagcg agttcaccct cgccttcccc gcggccaagc gggcggcgac gcacgccgcg
     3361 caggcgttcc taaagaagtt ctccgaggaa tacgccagag aacgcaagaa gacgcgaaag
     3421 taaacgggaa gccggaatcg gagagtagtt ctccggcggg gctggttcct gtgccctctc
     3481 cggtttggcg cagtgggttg ccggtagtaa gatatccctt acaaattcta gtagataccc
     3541 acgggagaat tatatggcgg agaacgggca gtcgacagga actttcaggg taaagagcgg
     3601 tatggcgcag atgctcaagg gcggcgtcat catggacgtg gtcgacgccg agcaggccag
     3661 gatcgccgag ga
//
LOCUS       DBNGVD010000484         3226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3580014, whole genome shotgun sequence.
ACCESSION   DBNGVD010000484 DBNGVD010000000
VERSION     DBNGVD010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3580014"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC16CM_13065"
     CDS             <1..606
                     /locus_tag="AC16CM_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3140810.1"
                     /translation="ASRAGYRQFLEAGVRIFEYGGPMIHAKTLVADGWLSKVGSTNLN
                     FSSLAANWEIDLVVEDTGFASQMEELFENDISNSREVRLESTGRGPRVRPQGPVDTSD
                     SRARAGVVGSGTGSGATLSRVVPTIVQKGTAPLQTHEHAMAVAASGAILGASLLGARF
                     PRLIAWPVTAVGAFLGGLGVVRAIQSTLSDRERERPDSGNT"
     gene            622..834
                     /locus_tag="AC16CM_13070"
     CDS             622..834
                     /locus_tag="AC16CM_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140811.1"
                     /translation="MEQEGFRMVEDVLVAAPNEASLRLQVTDLLLDGLRQVRAGRLPL
                     EQSDVLQEIEAALATYGDYFYPKNRR"
     gene            923..2311
                     /locus_tag="AC16CM_13075"
     CDS             923..2311
                     /locus_tag="AC16CM_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HIR3140812.1"
                     /translation="MEFRGTTEDAGRPGSDYQQRRERGHDARRVVPRGSHAGWALAPD
                     RPDPIALLEEQARGRLEELMPLRYERMMASPFAFMRGSAVIMANDLARTPNTGIQAQL
                     CGDAHLLNFGAYASPERSLLFDLNDFDETLPGPWEWDVKRLAASFVVAGRDNGFDAAG
                     CREAAQEAVASYRRRMAAFSEMGELEVWYSRIGEREVTGLLSDARSRKKTTKKLSKNV
                     QKARGRDSLQALSKLTGIVDGRRRIIDDPPLLVRIPEGDEIRTQINAILESYKRTLQE
                     DRRHLLDRYRFVDAARKVVGVGSVGTRAYVVLLEGRDQDDPLFLQVKEAGTSVLEAHV
                     PANIHEHHGHRVVAGQRLMQAASDIFLGWFRGTEGRDYYWRQLKDMKGSAKVESMSPD
                     ELVLYAGLCGWALARAHARSGDRVQIASYLGKSDRFDRAVADFAVAYADQNERDHAAL
                     CAAVKSGRLPKD"
     gene            2401..2997
                     /locus_tag="AC16CM_13080"
     CDS             2401..2997
                     /locus_tag="AC16CM_13080"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3140813.1"
                     /translation="MDDLYPLIENYGYLVVFFGVMLGTMGIPFPSAAILLAAGVLVQQ
                     GHLGLEGAILFGVLGAIVGNQIGYWIGYQAGRPFVLKWGRHVKLTPERLEWVERLFAR
                     HGGKAVFAGRFFAISRVLEALVAGTSRMRWGTFVLYSVLGGVVWATAVILAGYLFGQG
                     WGAAQNWSERSPLLLVVLLLLAPGAYLGYRWATSHRRR"
     gene            complement(3017..>3226)
                     /locus_tag="AC16CM_13085"
     CDS             complement(3017..>3226)
                     /locus_tag="AC16CM_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3140814.1"
                     /translation="LPDGNGIDLVREINDGLTDSIPTLVLTAGLDHSVAARAVEAGAQ
                     GAQSKAVSMTETVEAIKRLTGHRER"
BASE COUNT          575 a         1007 c         1081 g          563 t
ORIGIN      
        1 gcctcccggg ccggataccg gcagtttctg gaggctgggg tcaggatctt cgagtacgga
       61 gggcccatga tccacgccaa gacgctagtg gccgacggct ggctatcgaa ggttggctcg
      121 accaacctga acttcagcag cctggccgcc aactgggaga tagacctggt ggtcgaagac
      181 accggattcg cctcccagat ggaagagctg ttcgagaacg atatctccaa cagcagggag
      241 gtccgcctgg aatctacggg tcgtggtcct agggtacggc cgcagggtcc tgttgacacc
      301 tcggatagca gggcgcgcgc cggtgtcgtc ggcagcggta cggggtctgg ggccacgcta
      361 tccagggtgg tgcccacaat cgtccagaag ggcacagccc ccctgcaaac ccacgagcat
      421 gccatggccg tcgccgcgag cggggccatt ctgggggcat cgttgctcgg cgcgcgtttt
      481 ccgcgtctga tcgcctggcc cgttacggcg gttggcgcct tcctcggggg gctcggcgtg
      541 gtgcgggcga tccaatcgac gctctccgac cgtgagaggg agcggcccga ttccggcaat
      601 acgtagggaa gggtggagga gatggaacag gaaggattcc ggatggtcga agacgtgctg
      661 gtggccgctc ccaacgaggc atcgttgcgg ttgcaggtga ccgacctgct ccttgatggg
      721 ctgcgtcaag tgcgcgcggg aaggttgccc ctggagcaga gcgatgtctt gcaggagatc
      781 gaggcggcgc ttgcaaccta cggtgactac ttctacccaa agaatcgtcg ctaacgaagc
      841 ggccggcggg agcaatcggt ttccctgacc ctaattgccc caaggaggac tagtgaaaat
      901 cgagcaagac gcgaactccc cggtggaatt ccggggaacg accgaagatg ccggcaggcc
      961 tggatccgac taccagcaac gccgagagcg cggacatgac gcgcgtcgtg ttgtcccgag
     1021 gggcagccac gcggggtggg cgctagcacc cgaccggccg gacccgatag cactcctcga
     1081 ggagcaagcc agggggcggc tcgaagaact catgcccctc cggtacgagc gtatgatggc
     1141 ctccccgttc gcgttcatgc gcgggtctgc cgtcataatg gccaacgacc tcgcgcgcac
     1201 gccgaatacc ggcatccagg cccagctgtg cggcgacgcc cacctcttga atttcggggc
     1261 ttatgcctcg ccggaacgct ccctgctctt cgacctaaac gacttcgacg agaccctgcc
     1321 aggaccctgg gagtgggacg tcaaacgact cgcggcgagc ttcgtggtgg cgggccgcga
     1381 taacggcttc gacgcggctg gttgccgtga ggccgcgcag gaagccgtgg cgtcctatcg
     1441 gcggcggatg gccgcgttct cggagatggg agagctcgag gtctggtact cgcgcatcgg
     1501 agagcgggag gttaccggcc ttctctccga cgccaggtcc agaaagaaga ccaccaagaa
     1561 gctgagcaag aacgtgcaga aagcgcgcgg ccgcgacagc ctgcaagccc tctccaaact
     1621 aaccggcatt gtagatggac gccgcaggat aatcgacgac ccacccctgc tcgtgcgcat
     1681 accggagggg gatgagatca ggacgcagat caacgcgatc ctggagagct acaagcgtac
     1741 cctgcaggaa gaccggcgcc acctgctcga ccggtaccgc ttcgtggacg cggccagaaa
     1801 ggtcgttggg gtcggtagcg tgggcaccag ggcctacgtc gtgctcctcg aagggcgcga
     1861 tcaggatgat cccctgttcc tgcaggtaaa ggaggcggga acctcggtcc tggaggccca
     1921 cgttccggcc aacatccacg agcatcacgg acaccgggtc gtggccgggc agcgcctgat
     1981 gcaggccgca agcgacatct tcctcggctg gtttaggggc acggaggggc gcgactatta
     2041 ctggcgccaa ctcaaggata tgaagggctc ggcgaaggtc gagagcatgt cgcccgacga
     2101 gcttgtcctc tacgctgggc tttgtggctg ggcgctggcc cgggcgcacg cccgctccgg
     2161 agaccgcgtg cagatcgcct cctacctcgg caagagcgac cgattcgatc gcgcggtcgc
     2221 cgacttcgcc gtagcatacg cggaccagaa cgagcgggac cacgccgccc tgtgcgcggc
     2281 cgtgaaatcc ggaaggctac ctaaggacta gggaaggtat cgggaggatc aaggtgcgaa
     2341 agctcccagt gtccccgctc cccgttcccg aaccctggaa ctcactatac tgcctatgct
     2401 atggacgatc tctacccgct gatcgagaat tacggctacc tggtcgtctt cttcggcgtg
     2461 atgctcggga cgatgggtat ccccttcccg agtgcggcca tcctcctcgc cgcaggggtg
     2521 ctggtacagc agggacatct cggcctcgag ggtgccatat tgttcggtgt cctcggggcc
     2581 atcgtgggca accagatcgg ctactggata ggctaccagg cgggaaggcc gttcgtgttg
     2641 aagtgggggc gacacgtgaa gctcaccccc gagcgactgg agtgggtgga acggctcttc
     2701 gctcgccacg gcggcaaggc cgttttcgcc ggccgcttct tcgcgatctc gcgcgtactg
     2761 gaagcactcg tggccggcac gagccgcatg cgctggggca ccttcgttct ctacagtgtc
     2821 ctcggcgggg tggtatgggc caccgctgtc atcctggccg ggtatctgtt cggacagggt
     2881 tggggcgcgg cgcagaactg gtcggaacgc tctccgttgc tgctggtcgt cctgctcctg
     2941 ttagcgcccg gtgcctacct gggctaccgg tgggcgacct cccacaggag aagatagccc
     3001 ggaccggcag gtgctcttac ctctcccggt gcccggttag ccgcttgatc gcctcgacgg
     3061 tctcggtcat cgataccgcc ttggattggg caccctgcgc gcccgcctca acagcccggg
     3121 cggcaactga gtggtccagc cccgccgtca atactagcgt cgggatgctg tccgtcaggc
     3181 cgtcgttgat ctctcgtacc aggtcaatcc cgttcccgtc cggtaa
//
LOCUS       DBNGVD010000485         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1395394, whole genome shotgun sequence.
ACCESSION   DBNGVD010000485 DBNGVD010000000
VERSION     DBNGVD010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1395394"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="AC16CM_13090"
     CDS             <1..1595
                     /locus_tag="AC16CM_13090"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3140815.1"
                     /translation="DLMEGVANEAASLAGQNALGKLVYYYDDNRITIDGTTAISFDGE
                     DKGKRFEAQGWHVQHVSDSEDLEALAEATQNAIDEEGRPSLVIVRSHIAYPAPNATDT
                     AAAHGSALGEDEVRAAKEALGLDPDGHFVVPDEVYEHMDQKQRGRELEDEWNGRFDAW
                     SGEFPDLLEEWEQVWSGNPAPGYVEKLPKWDPSETEKLATRKAGGAAMDAFGEYAPTM
                     IGGAADLVGSTNTTFSNGGVFSPQNAGRNIAFGIREHAMGSAINGVALHGGMVRPYGS
                     TFLIFSDYMRPAVRLSALMGTKVAWIWTHDSVGLGEDGPTHQPIEHYMALRAIPNLTV
                     IRPADANETSVAWQSVFEADGPVALLLTRQNVPVLDPGPAEGVLRGAYVREDSEGGEP
                     EVILIGTGSEVAVALEAKGLLSEQGIDARVVSMPSWEIFDAQDQDYKDSVLPPSVEAR
                     VSVEAGVPTGWERYVGFRGRSVGINRYGASAPGEELLQELGISPENVANEALDLLGRA
                     ESVSEEEGTPAVEDTDPSEGHS"
     gene            1686..>2443
                     /locus_tag="AC16CM_13095"
     CDS             1686..>2443
                     /locus_tag="AC16CM_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HIR3140816.1"
                     /translation="MNENLEKAAQLGQGIWIDSISRDDLVEGGALHQMIEDGVVGVTS
                     NPSIFQKAISESELYDEQLEEASKETDDPKEIFWELAVRDIQDACDILAPVYERTAKK
                     DGYVSLEVSPDIGYDAPRTVDEALMLHDLVDRPNLLVKIPATQPGLAAIEEVIRNGKS
                     VNVTLIFSLERYKAVARAYIRGLQRLVAEGGDPSGVASVASFFVSRVDSEADGRLEEV
                     GRPDLQGRLAIANAKLAYEAFEEIFSGDDWTFLES"
BASE COUNT          469 a          798 c          817 g          359 t
ORIGIN      
        1 gcgacctgat ggagggcgtc gcaaacgagg ccgcctcgct ggccgggcag aacgccctcg
       61 gcaagctcgt ctactactat gacgacaacc gcataaccat cgacggcacg accgccatct
      121 cctttgacgg cgaggacaag ggcaaacgct tcgaggcgca ggggtggcac gtccagcacg
      181 tctccgactc cgaggacctc gaggccctcg ccgaggccac gcagaacgct atagacgaag
      241 agggacgtcc ttcgctcgtc atagtgcgct cgcatatcgc ctaccccgcc ccgaacgcca
      301 cggacaccgc cgccgcccac ggctcggccc tcggggaaga cgaggtcagg gcggccaagg
      361 aggccctcgg cctcgatccc gacgggcact tcgtggtccc ggatgaggtc tacgaacaca
      421 tggaccagaa acagcgcgga cgcgagctcg aagacgagtg gaacgggcgt ttcgacgcct
      481 ggagcgggga gttccccgac ctgctcgaag agtgggagca ggtctggagc ggcaaccccg
      541 cgcccggcta cgttgagaag ctgccgaagt gggacccgtc cgagaccgag aagctggcga
      601 cccgcaaggc cgggggggcg gcgatggacg ccttcgggga gtacgcccct accatgatcg
      661 gcggcgccgc agacctcgtc ggctcaacca acaccacctt ctcgaacggc ggggtcttct
      721 ccccccagaa cgccggtcgt aacatcgcct tcggcatccg cgagcacgcc atggggagcg
      781 ccatcaacgg cgtcgccctg cacggtggca tggtacggcc ctacggctcc acgttcctca
      841 tcttctccga ctacatgcgt ccggccgtgc ggctctccgc gctcatgggc accaaggtag
      901 cctggatctg gacgcacgac tcggttggac tcggtgagga cggcccgaca caccagccca
      961 tcgagcacta catggccttg cgggccatac ccaacctgac ggtaataagg ccggccgacg
     1021 ccaacgagac ctcggtggcc tggcagagcg tcttcgaggc cgacggcccc gtggcgctgc
     1081 tcctgacccg ccagaacgtc cccgtcctcg accctggtcc ggccgaaggc gtcctgcgcg
     1141 gcgcctacgt cagggaggac tcggagggcg gcgagcccga ggtcatcctt atcgggaccg
     1201 gctccgaggt cgccgtggcg ctagaagcga aggggctgct cagcgaacag ggtatcgacg
     1261 cccgtgtggt cagcatgcca tcctgggaga tcttcgacgc ccaggaccag gactacaaag
     1321 actccgtcct gccgccctcc gtcgaggcgc gcgtctcggt cgaggccggg gtgccgaccg
     1381 ggtgggagcg ctacgtcggc ttccgtggca ggagcgtcgg gatcaaccgc tacggcgcct
     1441 ccgcgccagg tgaggagctg ctgcaagaac tcgggataag cccggagaac gtggcgaacg
     1501 aagccctcga tctcctggga cgcgcggaga gcgtcagcga ggaagaaggc acgcccgcag
     1561 tagaggatac ggacccttcg gagggacact cctagagata ccgtgggatc ggtcttgagg
     1621 gcggcggcaa agggacgggg agcccatctg gttccctgtt ttcccaggag aacaggaggc
     1681 acgaaatgaa cgagaacctc gagaaggcgg cgcagttggg tcagggaatc tggatagact
     1741 cgatcagccg cgatgacctc gtcgagggcg gtgctttaca ccagatgatc gaggacggcg
     1801 tcgtgggggt gacctcgaac ccctccatct tccagaaggc catctccgag tcggagctct
     1861 acgacgagca gctcgaagag gcctccaaag agaccgatga cccgaaagag attttctggg
     1921 agctagccgt cagggatatc caggatgcct gcgacatact ggcccccgtc tacgagcgca
     1981 cggccaaaaa ggacggctac gtctctctcg aggtatcccc ggatatcggg tacgacgccc
     2041 cgaggaccgt ggacgaggcg ctcatgttgc acgatctggt cgaccggccg aacctgcttg
     2101 taaagatacc ggccacgcag ccggggctcg cggccatcga ggaggtcatc cgcaacggca
     2161 agagcgtcaa cgtcaccctg atcttctccc tggagcgcta caaggccgtc gcccgcgcct
     2221 acatcagggg cctgcagcgt ctggtggccg agggcggaga cccctccggg gtggcgagcg
     2281 tggccagctt cttcgtctcg cgcgtggact ccgaggcgga cgggcggctc gaagaggtag
     2341 ggaggccgga tctgcagggc cggctcgcca tagccaacgc caagctcgct tacgaggcgt
     2401 tcgaggagat cttctccggg gatgactgga ccttcctgga gtc
//
LOCUS       DBNGVD010000486         5160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1395801, whole genome shotgun sequence.
ACCESSION   DBNGVD010000486 DBNGVD010000000
VERSION     DBNGVD010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1395801"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16CM_13100"
     CDS             <1..218
                     /locus_tag="AC16CM_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140817.1"
                     /translation="LVLVALAATVAIGCGAGGSSSSQPAERHGSKKAGSTEQRAEDQT
                     SSGERLGHPALGSADAAVVMTEFSDYQ"
     gene            231..668
                     /locus_tag="AC16CM_13105"
                     /pseudo
     CDS             231..668
                     /locus_tag="AC16CM_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            661..1521
                     /locus_tag="AC16CM_13110"
     CDS             661..1521
                     /locus_tag="AC16CM_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3140818.1"
                     /translation="MVEVSLLATFLGGLLSLLSPCSALLLPAFFAYAFQSRSQLVGRT
                     AVFYLGLCATLVPLGMGISAVSALVYGHRSTLIAVSGAVIILLGIVQIAGGGFAFGPI
                     ERLRGRIQGDSVVTTFLLGSVYGFAGFCSGPILGAVLTVAASSGSTLQGAGLLATYAA
                     GMAAPLFLMALLWDRLDLGHRRWLRGRELSLGPFRFHTTNLISGLMFVAMGFLFIVFE
                     GTSGLESLYAAGGATDLALSAQRWAEAFSQKLSLPTVLVIPGLLLAGYLLYRYSRRRK
                     KPSAKLKADR"
     gene            1573..2331
                     /locus_tag="AC16CM_13115"
     CDS             1573..2331
                     /locus_tag="AC16CM_13115"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3140819.1"
                     /translation="MELLLPDRFPDARTGYGYVRTIFDQVASGMRPATVSITPSTRHI
                     GVTRRDTFRPGFSDAVGAAGEMGYPVLVRSSGGGATAADLGTFGFSIIRPVDERESGR
                     GIRDRYDEAAGFVLGAFSRLGVKAEVGEVRDEFCPGDHSIRVGDGEAGMKVVGIAQRI
                     TRRATSVGGIVLVEGEHELARVLKGVYGAMRLPFRPGSVGSLRRAGYEGGTLRAIGAF
                     AAEAEHIYSAARTPVDEMTLASAGTEGEQYLIGP"
     gene            2505..3434
                     /locus_tag="AC16CM_13120"
     CDS             2505..3434
                     /locus_tag="AC16CM_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3140820.1"
                     /translation="MRRMMLFMIVALVAAAMIGASALPTFARQAKTKGEGRGNGATAC
                     LVPGVYETVQKAVDDPRCQTIQLVGNSIEAVTIDRNVTITGQNEDLPPIVGGSFGKPV
                     FTILPGNTVNFEDFHIDGGGGATVERGGAIYNQGTLVLTTMIVRNSTATDGGGIYNAG
                     TMTINGGIVFRNTATDKGGGIFNAGTLTLGQGISVNSNVATTDGGGVYNQGTLYVCSA
                     TVTENEAPPGTENDLSGTPAQQCPTGPPGGPQKGENGVLLAHRGKELCLPQAALKAHL
                     EHGDEVLNEKGCPDPTIKKGAHQGRPRHESAGV"
     gene            3685..4410
                     /locus_tag="AC16CM_13125"
     CDS             3685..4410
                     /locus_tag="AC16CM_13125"
                     /inference="COORDINATES: protein motif:HMM:NF020604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HIR3140821.1"
                     /translation="MQEPPRSYVLCGTPRTGSTLLCGLLSSTGVLGRPESYFREPDEV
                     AWAESFGLATGGGRVRYYRAFVNAARSAGTSENGVFGARIMWGSLGRMMEGLGRVPGR
                     PDLPVLEEALGPLTFVHLRREDIASQAVSWCRAEQTGYWQQDDVAAREPHQDIAQARL
                     LEEAIREHNAAWRAWFDAQGVEPHTVTYEQLVGDRRRVVQGIAARLAVELPGDWRPRS
                     PHRKQADGLNRAWADALRASMDR"
     gene            complement(4703..>5160)
                     /locus_tag="AC16CM_13130"
     CDS             complement(4703..>5160)
                     /locus_tag="AC16CM_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140822.1"
                     /translation="HFGSRAELMVATARYGDEVRGLEGRLAGYRAASSGVERLEAFVE
                     FWGNYIPEIHGIARAMLAARETDGAVAAAWDDRMGVVQEACRDIVERLEREGMLARGW
                     TLDEAAEMLWSLLSIGNWENLTLERGWTVDRYVENMKEVVRSVFVRNSY"
BASE COUNT          900 a         1733 c         1658 g          869 t
ORIGIN      
        1 ttctcgttct ggttgcgctg gcggcgacgg ttgcgatcgg ctgcggcgct ggggggtctt
       61 cttcgtcaca gcccgcggag cggcatgggt cgaagaaggc gggttctacg gagcagaggg
      121 ccgaggatca gacttcttcc ggggagcggc tcgggcatcc tgcactgggt tcggctgacg
      181 cggcggtggt catgacggag ttttcggact atcagtgacc gtactgcggc cagttcgccc
      241 gtgaggtgga accgaagctc tacgaaaagt acatcgagaa cggaacgctg aggatcgaat
      301 ggcgggactt tccctatctc ggcaaggagt cggtcgacgc cgccgtcgcc gccagggccg
      361 cccaggccca gggtagattc tgggaatacc acgacctgct atacgcgaac cagtcatcgg
      421 gctacagcga ggagaacctg cgcgcgctcg ccgaagaagc ggacctcgac atggggcgct
      481 tcgagcgaga ctacgagaac ggtagcctcg ccgagctggt gcagaaagat ttcgagcagg
      541 gccagaggat tggcgtgaac ggtacgccga cgttcataat aaacgaccgg gtgctggtgg
      601 ggttgcagcc cgtcggggtc ttcgagaagg ccatcgagga tgccaggcgg gaggcggagg
      661 gtggttgagg tctccctcct ggccactttc ctcgggggcc ttctctccct gcttagcccg
      721 tgcagcgcgc tactcctgcc cgccttcttc gcctacgcct tccagtctcg cagccagctc
      781 gtggggagga cggccgtctt ctaccttggg ctctgcgcca ccctcgtccc gctcggtatg
      841 gggatatccg ccgtcagcgc gctcgtctac gggcacagga gcacgctcat tgccgtctcc
      901 ggcgcggtca taatcctcct cggcatcgtc cagatcgcgg gaggcgggtt cgcgttcggg
      961 ccgatcgaac gcctgcgcgg caggatccag ggcgactccg tagttacgac cttcctcctc
     1021 ggatctgtct acggcttcgc cggtttctgc tcgggaccaa tcctgggcgc cgtcctgacc
     1081 gtcgccgcct cctcgggatc caccctgcag ggagccggcc tcctcgccac ctacgccgcg
     1141 ggcatggccg cccccctgtt cctgatggcc ctgctctggg accgcctcga cctcggccac
     1201 cgtcgctggc tccgcggccg cgaactctcc ctcgggcctt tccgattcca caccacgaac
     1261 ctcatctccg gccttatgtt cgtcgccatg ggcttcctgt ttatcgtctt cgaaggcacc
     1321 tcgggcctcg aaagcctcta cgccgccggc ggcgccacgg acctcgccct ctcggcccag
     1381 cgctgggccg aagccttctc ccagaagcta tcgctcccga ccgtgctggt gatccccggc
     1441 ctcctgctag ccgggtacct cctctacagg tactcgcgac gccggaaaaa gccatcagcc
     1501 aagctgaaag ctgaccgctg aaagctaaca agctaacaag ctgacagctc ccttccgctg
     1561 tagactctgt ctatggagct tctcctaccc gaccgcttcc ccgatgccag gacgggctac
     1621 ggctacgtgc ggactatctt cgaccaggtc gcttcgggga tgcggcctgc gacggtttcg
     1681 atcacgcctt ccacccgcca tataggggtg acgcgccggg atacgttccg ccccgggttc
     1741 tccgacgccg tcggggcagc gggcgagatg ggctaccccg tgctggtccg ctcctcgggt
     1801 ggcggcgcga ccgccgctga cctgggcacg ttcggcttct cgatcatccg ccccgtagac
     1861 gagagggaat ccgggcgcgg cataagggac cgctacgacg aggcggcggg cttcgtgcta
     1921 ggcgccttct cacggctggg ggtgaaggcc gaggtggggg aggtgcgcga cgagttctgc
     1981 ccgggagacc acagcataag ggtcggggac ggggaggccg gcatgaaagt cgtcggcatc
     2041 gcgcagagga tcacccgccg cgccacgagc gtcgggggca tcgtgctcgt ggagggagaa
     2101 cacgagttgg cccgcgtcct gaaaggcgtc tacggcgcca tgcgcctgcc cttcaggccg
     2161 ggtagcgtcg gcagcctccg ccgcgccggc tacgagggag gcaccctacg ggccatcggg
     2221 gccttcgccg ccgaagccga gcacatctac agtgccgcac gcacccccgt cgacgagatg
     2281 acgctggcat ccgccggcac cgagggagag cagtacctga tcgggccatg aaccagactt
     2341 ccggggccgc agccgggcct cgcgaggcaa cgagcaatac tcccgaagcc gaaacccgta
     2401 gccttcccga cgagttgcgc atgtcttccg gagccgtcac acttgtctcc gttgtatgag
     2461 ttggcgtggc cgggtagccc cgctctttac tggaggtcgc gcttatgagg cgcatgatgt
     2521 tgtttatgat agtggccctg gtggcagcgg cgatgatcgg ggctagcgcg cttcctacct
     2581 tcgccaggca ggccaagacg aagggggaag gacgggggaa tggtgcaacc gcatgtctcg
     2641 tacccggtgt gtacgaaacg gtccagaagg ccgtagacga cccgcgttgc cagaccatac
     2701 agcttgtggg gaactccatc gaggccgtaa ccatagaccg gaacgtaacg atcacggggc
     2761 agaacgagga tcttccaccc atcgtcggcg gctcgttcgg caagccggtc ttcaccatcc
     2821 ttcctggtaa taccgtcaac ttcgaggact tccacatcga tggtggcggc ggggccaccg
     2881 tcgaacgggg cggggcaatc tacaaccagg gcacgctcgt cctcacgacc atgatcgttc
     2941 gaaacagcac cgccaccgac ggcgggggca tctataacgc tggcaccatg acgatcaacg
     3001 ggggaatagt cttccgcaac accgccaccg acaagggagg cggcatcttc aacgcgggta
     3061 ccctgacgct gggccaaggg atctccgtaa actcgaacgt ggccaccaca gacggtggcg
     3121 gcgtctacaa ccagggcacc ctctacgtgt gtagcgcgac cgtcacggaa aacgaggctc
     3181 ctcctggcac cgagaacgat ctctccggca caccggccca gcagtgcccc acgggcccac
     3241 cagggggacc acagaaggga gagaacggcg tcctgctcgc ccacaggggc aaggagctgt
     3301 gcctgcccca ggcagcgctg aaagcccacc tcgaacacgg cgacgaagtg ctgaacgaaa
     3361 aaggctgccc ggatcccacc atcaagaagg gagcgcacca gggcagacct cgccatgaat
     3421 ccgccggcgt atagccggat ccggggcggc agaaggacgc cgtcgcggtg cgccgcgtag
     3481 gctttcgggg tgcgtatcgt cttccagctt gggtaggccg ccgaccgcaa tcgaccgaga
     3541 cctggtggaa gacgcccagg tccctcgcct tggtccggcg cttcttccag cttggaatga
     3601 tatagaggag gccgagaagc ggtggagctc ctccgcttgg cataatgcgg cctgtcggca
     3661 atggaacgat gggggttgca tctgatgcag gaaccgccgc gctcgtacgt actctgcgga
     3721 acgccgcgga cggggagcac cctgctgtgc ggcctgctct cttcgaccgg cgtgcttggc
     3781 cggcccgagt cgtacttccg cgagccagat gaggtcgcgt gggcggagag cttcggcctc
     3841 gccaccgggg gcgggcgcgt ccggtactac cgcgccttcg tgaacgcggc ccgaagcgcc
     3901 ggcacgtcgg agaacggcgt gttcggcgcc cggatcatgt gggggtcgct cgggcgcatg
     3961 atggaaggtc tgggtcgggt gccggggagg cctgacctac cggtcctcga ggaagcgctc
     4021 ggtcccctga ccttcgtcca cctgcgacgc gaagacatcg cgtcgcaggc cgtctcgtgg
     4081 tgccgagccg agcagaccgg gtactggcag caagacgatg tcgccgctcg ggagccacac
     4141 caggacatcg cacaagcgag gctattggag gaggcgattc gggagcacaa tgccgcctgg
     4201 cgggcatggt tcgacgccca gggcgtcgag ccgcacacgg tcacctacga gcaactcgtc
     4261 ggtgatcgca ggcgtgtggt ccagggcatc gccgcaaggc tggcagtcga gctacccggc
     4321 gactggcggc cccggtcgcc gcaccgcaag caggccgacg ggctgaaccg agcctgggcc
     4381 gacgccctgc gcgcgtcgat ggatcgctga tttacgagac caccgccaga taccttgcga
     4441 cttgaggggc aggggcgctt cgcgaagcgc ccacctgtca ccaacaggct ataggcatca
     4501 ggtcttcgat gcaatcccga ctcgtcggga ttgcatcgct ctatagaacc aggaaatcag
     4561 cgccgagcca ccccccccgt cattcccgcg aaagcgggaa tccacgaacg tcggaagtaa
     4621 cgggctccgt gcgtttcggc gttcccgtga cccggagaag gcatcaggta tattttcctg
     4681 aaacctgaaa cctgatgcct ccctagtacg agtttcgcac gaagacgctc ctcaccacct
     4741 ctttcatgtt ctccacgtac cggtcgacgg tccagccccg ctcgagcgtg aggttctccc
     4801 agttgccgat ggacagcagg ctccagagca tctccgctgc ctcgtccaac gtccaccccc
     4861 gagccagcat cccctcgcgc tctagccgct ccacgatgtc cctgcaggcc tcctgcacca
     4921 cgcccatccg gtcgtcccag gctgcggcca cggccccgtc ggtctctcgg gcggcgagca
     4981 ttgccctggc tatgccgtgg atctccggta tgtaattccc ccagaactcc acgaacgcct
     5041 cgagccgctc caccccgcta gaagccgccc ggtagccggc cagcctaccc tccagccccc
     5101 gcacctcatc accgtaacgc gccgtggcca ccatgagctc ggccctggaa ccgaagtggt
//
LOCUS       DBNGVD010000487         5014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1212785, whole genome shotgun sequence.
ACCESSION   DBNGVD010000487 DBNGVD010000000
VERSION     DBNGVD010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1212785"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16CM_13135"
     CDS             complement(<1..266)
                     /locus_tag="AC16CM_13135"
                     /inference="COORDINATES: protein motif:HMM:NF023580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR3140823.1"
                     /translation="MSEIPTQSQQLTAVGVVTGHTSRSSYGQDHRRTLLFPSVRFQAA
                     DGRTVDFQSKIGSNAPPKVGEQVTVYYDPERPEDAKLSLGSTFRP"
     gene            complement(525..1223)
                     /locus_tag="AC16CM_13140"
     CDS             complement(525..1223)
                     /locus_tag="AC16CM_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3140824.1"
                     /translation="MRRQQVYWSVGALLAISALGGLILSWPPGAPADSGSQLGAALLG
                     AAIVGLVILVSEYLLSSLVSTRMSEIENRNSSAARERELRREEVAEALEQHRGDRIDK
                     WVLQFMASFQQDLRRIDLSGRDMSGLYFGACNFLRANLKGSDLRGANLHGAYLAWAYL
                     SEATLEGADLGDADLAGAGLEGANLSGANLCGTNLTRVHLTGAQLDGARYDRRTVWPE
                     GFEPHGSGAVSLHG"
     gene            complement(1682..2107)
                     /locus_tag="AC16CM_13145"
     CDS             complement(1682..2107)
                     /locus_tag="AC16CM_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140825.1"
                     /translation="MGEIDRQRHEPGPEGLDYVKVGLGLVFVFGAVVFGILAPPGTLV
                     ISFGLMIPAVALIATALQETRKRRTIRPPDKERELLSAIRDNGGSITPAEAAMETSLT
                     VRDADRMLSGLAGGGHLSVERAGGTLRYSLPGRPAGELD"
     gene            2433..2816
                     /locus_tag="AC16CM_13150"
     CDS             2433..2816
                     /locus_tag="AC16CM_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140826.1"
                     /translation="MRRLMIMTAMTMLLVGVFAAAAYARTFTCTDLPCYGTSDRDVID
                     ERPASGTPDEIFGRAGADNINAGIYPSDRDILRGQNGNDRLKTDDGDGRDRAYGGEGT
                     DTCRVDEGDAYFGCEAVYVDGVFLP"
     gene            complement(3307..4380)
                     /locus_tag="AC16CM_13155"
     CDS             complement(3307..4380)
                     /locus_tag="AC16CM_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140827.1"
                     /translation="MLVLLPAIVAVVLSASATALAASPGESDPAKGTPGKGQLGKNKD
                     FSGLVDIGGGREMYMECRGNGSPTVVLVSGARDRAETWSKTRDPAEQALLPAISETNR
                     VCVYDRPGTYLADGEGTGDFLPSRSDPAPQPTTLQDAVNDLHALLGASGERGPYVLVG
                     HSMGGAISKLFASEYPEYVSGLVLIDYTPYEARKALTDRQWGYWKVLLGNPPEEAIDL
                     YPAVERFRHQRNLEQALITAPLKPMPLIVLSSDEPYDLIPYVKDGTLPLTADQAERLG
                     ELLFRVIVEARANLVSQVPGARHITETHSGHYIHQEQPRLVIDSVREVVEAARKKSCA
                     LGIKNHGQCVKAQKRASSNGSLP"
     gene            complement(4455..4928)
                     /locus_tag="AC16CM_13160"
     CDS             complement(4455..4928)
                     /locus_tag="AC16CM_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR3140828.1"
                     /translation="MFQLAWAHAHETLLWASKTRGARHTFNYDLINSPDPTSQLSSAW
                     LIPAVPMREKTHGRHPTQKPLRLLRRAVLASTREGDLVFDPFSGSATTGVAAKELGRF
                     FVGAEKQREYAVLAGRRIAAVGRGAVVCQEILKSSPFTRCAHLSLRSMLPCPAAS"
BASE COUNT          905 a         1579 c         1588 g          942 t
ORIGIN      
        1 ggcctgaacg tggagccaag agagagcttc gcatcctcgg gccgctcggg atcgtagtag
       61 acggttacct gctctcccac cttcggcggg gcgttggaac cgatcttgct ctggaaatcg
      121 accgtcctgc cgtcagccgc ctggaaccgt acggacggga agaggagcgt cctccggtga
      181 tcctggccgt aagagctccg agacgtgtgg ccggtaacca cgcctaccgc cgtcagttgt
      241 tggctctgcg taggtatctc gctcaaggtc ctctcctcac ctccaggcgc ctggtgtcaa
      301 agaccaccgc ccattgcgcc gacctgtaag ggcagcctag ccgcccgcaa ccgggggagc
      361 atcccccaaa tggactattt tccgcgcggc tcgcccgatg gagaggtttc tgggcgtggg
      421 cacacgcaag tctgtgcaac gccgagggtt gggtccgctt ctatctcgcg ccgaggggct
      481 ggtgggagac gtgcggtttt tccgggcgta ccgggtcagc gtgtttaccc gtggaggctt
      541 acggcaccgc taccatgggg ctcgaatcct tctggccata cagtacggcg gtcgtatctc
      601 gcaccgtcga gctgtgcgcc ggtcagatgc acccgcgtga ggttcgtccc gcagaggttt
      661 gctcccgaaa ggtttgcacc ctccaggccg gcacccgcga ggtctgcgtc tccgaggtcg
      721 gcaccttcga gggtcgcttc gctgaggtag gcccaggcca ggtaggctcc gtggaggttc
      781 gccccacgca ggtcactacc cttcaggttg gcccgcaaga aattgcaggc cccgaagtag
      841 agccccgaca tgtcacggcc gctcaggtct atgcgcctca ggtcttgctg aaagcttgcc
      901 atgaactgca ggacccactt gtcgatcctg tcaccacggt gctgctctag cgcctctgca
      961 acctcttcac gccgcagttc gcgctctcgg gctgcagaag agttgcggtt ctcgatctcg
     1021 ctcatccgcg tgctcacgag gctgctcaag agatactcgg agaccagtat caccaggccg
     1081 acgatcgccg ccccgagcaa tgcggcaccg agctggctgc cgctatcggc cggcgctcca
     1141 ggcggccacg acaggatcag accacccagc gcgcttatcg ccagtagcgc cccaacagac
     1201 caatatactt gctgacgccg cacaagctca ccccctactc acggcccccg gtgtccggtc
     1261 gaaaactgcc gcagacaccg gagcgcctcg ctccctacca ggctcctcac ggagaatacc
     1321 aatcatcaga ccgcgcgacc tcggaaacgg tgacacataa gaacgctttc cgagatagca
     1381 cgccattctc tcatcgctcg cgaaacctcg ctttccccca ggcaagctcc ggcaaacctt
     1441 tctgatgcag gcacggatcc cgatcggcaa gacctgggcc aaagtccttc ttccggcaag
     1501 tcgccggcgc agaggcgtgc ctttgctctt gcccgcctcg agcaacgtac catcagcgcg
     1561 agccatcgca agtcgggacg atgcctcgtg tggggtttcg tgctacgctc gtggccggcg
     1621 ggatgccggt agaacttaga ggtatctccg gcgcgcggag gcgttggtgc ggacggtatg
     1681 gtcagtcgag ttcgccagcc ggtcttccgg gcagagagta acgcagcgtc ccgccagctc
     1741 tctccacaga gaggtgccca cctcccgcca acccagacag catcctgtcc gcgtccctga
     1801 cggtcagcga ggtctccatt gcggcctccg ccggggtgat cgagcccccg ttatcccgga
     1861 tggccgacag caactcgcgc tccttgtccg ggggacgtat cgtgcgccgc tttctggttt
     1921 cttgcagggc tgtggcgatg agcgcgacag ccgggatcat caggccgaag gagatcacca
     1981 gcgtaccagg cggggcgagg atgccaaaga cgaccgcgcc gaagacgaag acgaggccaa
     2041 ggccaacttt gacatagtcg agaccctctg gccccggctc atgccgctgg cggtctatct
     2101 cacccaaact tcacctccat agccgcgtac tcgaaggtta ccggatattg taatccacct
     2161 tgcaatagca aacgcggttt cttccgctcg cgtccgggcc gaagcgagtc aggatatggc
     2221 cgttcggtca cgagaactag ccgttcggac gatgagccgc ctccccacaa gacaggtaag
     2281 ctactgtacg acacatggag tagggtgagg gcctcgcgtt gcgtcccccg cccggaccaa
     2341 gaaaggcggt ggcgatgaga agcatcggct cgttgttggc ctcgggagac gtgggcgccc
     2401 acgtcttctc cgtacagaga ggagagatgt agatgcgcag gttgatgatc atgacggcga
     2461 tgacaatgct gctggttgga gttttcgccg ccgccgctta cgcgcgcacc tttacttgca
     2521 ccgatctgcc ctgttacggt acctcggatc gtgacgttat cgacgagcgt cccgcctccg
     2581 gtacacccga cgagatattc ggccgggcag gcgcagacaa cataaacgca gggatctatc
     2641 cttccgacag agacatcctt cgcggccaga atggcaacga taggctcaag accgacgacg
     2701 gtgacggtcg cgacagggca tacggggggg aaggcacaga tacctgtcgc gtcgatgagg
     2761 gtgacgcata cttcggctgc gaagcagtgt atgtagatgg cgtgttcctg ccctgacacc
     2821 gcgctgtatg aaaactggcc gagcgttcag agttaacgca agggacgtgt aagcagctga
     2881 gaccagaacc ggagagggca gctacggaag cgccgcctat ccaagaccgt ctcccccact
     2941 cccggcgagc agtagggcgg cccggggctc tttcgagctc cgggccgcat cgggctggcg
     3001 gagccgccaa caggcaagcc tgtgccggcc tgccaggcga gagggtcttc tgagcgtcta
     3061 ctcctctagt accttgcttg ctttgaggtt cttggtgtgg ccgatgcggc gggtcctgaa
     3121 gcaacggtgg atggtcttcc acggaggaaa gtcgtggggg agcgagcgtc gggcgaaccc
     3181 acagcgcgag ctaggagttg acgtcgagga cctcgcgcga ggcgcgtagc ccaggtcctc
     3241 ctgagccttc ggagcagggc agacaaacct ctatgtagga cgttcatcgt ggaagaggta
     3301 tgctagctag ggtagcgaac cattactcga tgcacgtttt tgggccttga cgcactggcc
     3361 gtggttcttg atcccgagtg cgcacgactt cttgcgggcc gcttccacca cctcacgaac
     3421 cgagtcgatc acgagccgcg gttgttcctg gtgtatgtag tggccgctgt gcgtctccgt
     3481 tatgtgcctg gcgccgggca cctggctcac gaggttggcc ctggcctcca cgatcacccg
     3541 aaataggagc tcgccgagcc gctctgcctg gtcggccgtc aggggcagcg tgccgtcctt
     3601 gacatagggt ataaggtcgt aaggctcgtc cgaggagagc acgattaggg gcatcggctt
     3661 caggggcgct gtgatcaatg cctgttcgag gtttcgctgg tggcgaaacc tctccacagc
     3721 cgggtaaagg tcgatggcct cttccggtgg attgcccagg agcaccttcc agtagcccca
     3781 ttgccggtcg gtcaacgctt tgcgcgcttc gtagggggtg taatcgatga ggacgagtcc
     3841 cgagacatat tcggggtatt cgctcgcaaa gagcttcgat atggcgcctc ccatcgagtg
     3901 gccgaccaga acgtagggac cgcgttctcc agaggcgccg agcagggcat ggagatcgtt
     3961 gaccgcatcc tggagcgtgg tcggctgggg ggccgggtcg cttcggctcg gcaagaagtc
     4021 gcctgtacct tcaccgtccg cgagataggt tccgggacgg tcatagacgc ataccctgtt
     4081 ggtctcggag atcgcgggca agagcgcctg ctcggccgga tcgcgcgtct tgctccacgt
     4141 ttcggcgcga tccctggccc ccgagacgag caccacggtg ggagagccat tgccgcggca
     4201 ctccatgtac atctcgcggc ccccgccgat gtcgaccagg cccgagaaat ccttgttctt
     4261 gccgagctgg cccttacccg gggtgccttt ggcagggtcg ctttcgcccg ggctagcagc
     4321 tagcgctgtg gcggacgcac tcagcaccac cgccacgatg gccggcagca acaccaacat
     4381 cgtcgtcttc ttcgacacac catctccaga ccctagaagg aggacaggta acgctcctgc
     4441 gcctacacgc agcgtcagct ggccgcggga caaggtagca tactcctgag tgaaaggtgg
     4501 gcgcatcgcg taaacgggct agatttcaat atttcttgac agactacggc gcccctcccc
     4561 accgcagcga tcctgcgccc cgccagcaca gcgtactctc gctgcttctc cgcccccacg
     4621 aagaagcgcc ccaactcctt ggccgctacc cccgtcgtag ctgacccgct gaaagggtca
     4681 aacacgaggt cgccttccct ggtggaggct agcacggcgc gcctgaggag gcgcaggggt
     4741 ttctgggtgg gatgacgccc gtgggtcttc tccctcatgg gcacggcggg gatgagccat
     4801 gcggaggaga gctgggaggt tgggtcgggg ctgtttatga ggtcgtagtt gaaggtgtgg
     4861 cgcgcgcccc tggtcttgct cgcccacagg agggtttcgt gggcgtgggc ccacgcaagc
     4921 tggaacaacg cctcgcgtgg cgttgcgcgg tgtggtcggt acggatggcg gggtggagct
     4981 tggcgttgcg ctgtagaaac gcgagccctg tcag
//
LOCUS       DBNGVD010000488         3478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4675475, whole genome shotgun sequence.
ACCESSION   DBNGVD010000488 DBNGVD010000000
VERSION     DBNGVD010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4675475"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16CM_13165"
     CDS             complement(<1..435)
                     /locus_tag="AC16CM_13165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3140829.1"
                     /translation="MAVELAGITKRFGQFVAVDDLTLDIYEGEFFSLLGPSGCGKTTT
                     LRMIAGFEEPTEGGISVSGDPVRGVPPYRRPVNTVFQSYAIFPHLNVFDNVAFGLRRS
                     GVKGEELQTRVTDACEMVQLSGFEQRKPNALSGGQQQRVALAR"
     gene            complement(502..1923)
                     /locus_tag="AC16CM_13170"
     CDS             complement(502..1923)
                     /locus_tag="AC16CM_13170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3140830.1"
                     /translation="MSGGPWSPIAAADVVIVGAGLAGLTAATRLDAAGVSVTVIEAQD
                     RVGGRTKAGSAGGVSLEMGGQWAGPEQRRILALAGEVGVGTFPADVPGRTVFYEGGRR
                     SEYDDEGEVPFADPGSPGEVERAFRELGELARGVPADAPWQADDAADLDGKTLETWKL
                     EHIQGDGARFYFDLAVESLYACEPRDVSLLGVLSDIASSGSFGGLFEIEASAEEYRFY
                     GGAAEISTRLARGLDRRVVVGSPVRRIVQEKDAVLVESDRASVRAKAVVVTVPPALRG
                     RIEYVPALPAAHDGLSQRMPMGAVIKCHAVYGAPFWREKGLSGRAEGDRGPCKITCDN
                     SPPGEEVGVLTGFVLGSDAREWGRREPRERERAVLECFARYFGDEALGPVAYAETDWG
                     AEIYSRGGYAGVPVPGMLLDHGQALAEPVGRIHWAGAETASAWNGYMEGAVESGERVA
                     REVLSGLDGTTQATTRQVWTYRR"
     gene            complement(2003..3427)
                     /locus_tag="AC16CM_13175"
     CDS             complement(2003..3427)
                     /locus_tag="AC16CM_13175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3140831.1"
                     /translation="MTSTARQPAHAGAAAKDYHSYSFWLETVGEDLTPRSSLPGPTDA
                     DVAILGAGFTGLWTAYYLLRQDPSLRVVVLESEIAGFGASGRNGAWCNSAFPVTPGEL
                     MRRFGRDATRDLLLEMRGAVDEVGRVAGVEGIDAHYFRGGQLRVARGAAQVPAIEDAY
                     QSARTLGLEEDLQLLDADETAERIRITGARASLYNPHCATIQPARLARGLARAVERLG
                     GEIFERTTVTDYEGGSNPRFSTEAGEVRAGAIVLAGEAYLARLRKLRRQVLPIYSLIV
                     LTEPLSEAQWAEIGWEGRECVASNRYTVDYLSRTADGRILFGGRGAPYHYGSSIQDEY
                     DSHEATHEMLRHTAQEWFPAIRGVRFTHAWGGPLAMPRDWMPTMSYDPSEGVATARGY
                     TGQGVATANLSGRTLADLILGRDTAVTSLPSVNHEPRSWEPEPLRWLGARYVQRGLAR
                     VDDQAERTGQPPTGKTLAERLGSH"
BASE COUNT          545 a         1255 c         1100 g          578 t
ORIGIN      
        1 cctcgcgagc gcgaccctct gctgctggcc gccggataga gcattaggct tgcgttgctc
       61 gaacccggag agctgcacca tctcgcatgc gtcggtgacc ctcgtctgca actcctcccc
      121 cttgacgcct gaccgtctca ggccgaaggc cacgttgtcg aagacgttga ggtgggggaa
      181 gatagcgtag gactggaaca cggtgttgac cggcctgcgg tagggtggca cgccgcgcac
      241 cgggtccccg gagacggaga taccgccctc cgtcggttct tcgaagccgg cgatcatgcg
      301 cagcgtagtg gtcttgccgc acccgctcgg gccgagcagc gagaagaact cgccctcgta
      361 gatgtctagc gtgaggtcgt ccacggcgac gaattgcccg aagcgcttgg tgatcccggc
      421 cagctctacg gccacgtccc tgcccttcgc ggcatccctc gcctcgggtg gatgctcggt
      481 tgttttcttg tcctgcaacc cttacctcct gtaagtccaa acctgcctgg tcgtggcctg
      541 cgttgtgccg tcgaggcccg agagcacctc ccgagccacc cgctctccgg attcgacggc
      601 accttccatg taaccgttcc atgcggaggc cgtctccgcg ccggcccagt ggatgcggcc
      661 caccggctcg gccagcgcct ggccatgatc cagaagcata cccgggacag ggacccccgc
      721 gtatccgcca cgcgagtaga tttcggcccc ccaatccgtc tccgcgtagg ccacggggcc
      781 cagagcctca tccccgaaat acctggcgaa gcactcgagc acggcccgct cccgctcccg
      841 gggctccctg cgcccccact cgcgggcgtc ggagccgaga acgaagccgg tcagaacgcc
      901 gacctcctcc cccgggggtg agttgtcgca ggtgatcttg cacggtcccc tgtcgccctc
      961 ggcgcgcccg ctcaaccctt tctcccgcca gaacggcgcc ccgtagacgg cgtggcattt
     1021 gatgacggcg cccatgggca tcctctgcga cagcccgtcg tgcgccgccg gaagcgcggg
     1081 aacgtactct attcgcccgc gcaacgcggg cggcacggta acgaccacgg ccttcgcccg
     1141 gacggaggcg cggtcagact ccacgagcac cgcgtccttc tcttgcacga tgcggcgcac
     1201 gggtgagccc accaccacgc gcctgtccaa cccgcgggcg aggcgggtcg agatctccgc
     1261 agccccgccg tagaacctgt actcctcggc ggaagcctct atctcgaaga ggcccccgaa
     1321 gcttccggac gacgcgatat cggagagcac cccgagcaga gagacgtcgc ggggctcgca
     1381 ggcgtatagc gactccacgg cgaggtcgaa gtagaacctg gcgccgtcgc cctgtatgtg
     1441 ctcgagcttc cacgtttcga gcgtctttcc gtctagatct gcggcatcat ccgcctgcca
     1501 gggcgcgtca gcgggcacac ccctggccag ctcaccgagc tcccggaacg ccctctcgac
     1561 ctcaccgggc gagcccggat cggcgaacgg gacctcgccc tcgtcgtcgt actccgagcg
     1621 gcgtccaccc tcatagaaaa cggtgcgacc gggaacgtct gcgggaaagg tcccgacccc
     1681 gacctcgccg gccagggcta ggatgcgacg ctgttctggg ccagcccact ggccgcccat
     1741 ctccaggctc accccgcctg cgcttcccgc cttcgtccga ccgccaaccc tgtcctgggc
     1801 ctcgatcacg gttaccgata cgccggccgc atcgagtctt gtggcggccg ttagcccggc
     1861 tagcccagcc ccgacgatca cgacgtcggc cgccgcaatc ggggaccacg ggccgccgct
     1921 catccgatcg cctccatccc gtcgctcctt gccttcaccc cgtctccttg tgcgaaagat
     1981 atcatatcgg ggaaatcgga ctctagtggc taccgaggcg ctcggccagg gtcttgccgg
     2041 tagggggttg cccggttctc tcggcctgat cgtcaaccct ggcgaggccg cgctgcacgt
     2101 acctggcgcc taaccaccgt aggggttcgg gctcccagga gcgaggctcg tggttgacgg
     2161 aaggaaggct ggtaacggcg gtgtcacggc cgaggataag gtcggccagc gttcggcctg
     2221 agaggttggc tgtggccacg ccctgcccgg tgtaaccgcg ggcggtggcg acgccttccg
     2281 aggggtcgta ggacatggtc ggcatccagt cgcggggcat agccagcggt cctccccagg
     2341 cgtgggtaaa ccggaccccc cgtatggctg ggaaccactc ctgcgccgtg tggcgcagca
     2401 tctcgtgggt tgcctcgtgg ctgtcgtact cgtcctgtat gctcgagccg tagtggtagg
     2461 gcgccccgcg gcctccgaag agtatccgcc cgtcggccgt tcgcgagagg tagtctacgg
     2521 tgtagcgatt ggatgccacg cactcgcggc cctcccagcc gatctctgcc cactgcgcct
     2581 ccgagagcgg ctcggtcagg acgataaggg agtagatggg gagcacctgc cggcgcagct
     2641 tgcgcagtcg cgccaggtac gcctcgccgg cgagcacgat cgccccggcc cgtacctcgc
     2701 cagcctccgt cgagaacctc gggttgctgc ctccctcgta gtcggtgacg gtggtccgct
     2761 cgaagatctc accgccgagg cgctccacgg ccctggccag cccacgcgcg agacgcgccg
     2821 gctggatggt cgcgcagtgc gggttgtaca gactcgccct ggcaccggtg atcctgatgc
     2881 gctctgcggt ctcgtccgcg tcgagcaact gcaagtcttc ttccaatccg agcgtccggg
     2941 cggactggta agcatcctct atggccggca cctgtgccgc cccgcgggcg acccgaagtt
     3001 gccccccacg gaagtaatgg gcgtctatgc cctcgacccc ggcgacccgg ccaacctcgt
     3061 cgacggcccc gcgcatctcg agcaacaggt ccctcgtggc gtccctaccg aagcgacgca
     3121 tgagctcgcc cggggtaaca ggaaacgccg agttgcacca cgccccattg cgtccggagg
     3181 ccccgaaacc ggcgatctca gactccagca ccaccacgcg caaagaaggg tcctgccgga
     3241 gcaggtagta agccgtccag agacccgtaa agccggcacc caggatggcc acgtcggcgt
     3301 cggtggggcc aggcagagaa gacctcgggg tgaggtcctc tcccaccgtc tcaagccaga
     3361 agctgtaaga atggtagtct ttggccgcgg caccggcgtg tgccggctgg cgtgcggtgc
     3421 tggtcaagcg actacccctc tagttcctgt agcccagttg ccgttccagc tcggtgag
//
LOCUS       DBNGVD010000489         6113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4311214, whole genome shotgun sequence.
ACCESSION   DBNGVD010000489 DBNGVD010000000
VERSION     DBNGVD010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4311214"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..169
                     /locus_tag="AC16CM_13180"
     CDS             8..169
                     /locus_tag="AC16CM_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3140832.1"
                     /translation="MRLVARGMSNQQIAESLVLSEHTVHRHVANVLRKLGVSSRASAV
                     AEAAQLGLL"
     gene            321..461
                     /locus_tag="AC16CM_13185"
     CDS             321..461
                     /locus_tag="AC16CM_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140833.1"
                     /translation="MEPRNGSTGRYRKSGVRAPIIQGWFLSEGVAIGEVVLSDYLCSV
                     PR"
     gene            590..>6113
                     /locus_tag="AC16CM_13190"
     CDS             590..>6113
                     /locus_tag="AC16CM_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3140834.1"
                     /translation="MPEEQNHSPHGASAENREEAGSAVIFHERSVDRLPDNLPPERTS
                     FVGRGREVAEIGKLLSERQLVTLCGPGGAGKTRLALAVARSLVDRFEDGVCWVGLASI
                     SDPELLPETVATALGVPETPDRSPTEALVDNLQGRRAFLILDNCEHLIEACADLADTL
                     LGSCPSLKLLATSREPLRVAGETNFMVPSLSVPDPGRSASTEELAGYEAVQLLLQRAR
                     DVNPGFRLTEENAATVVLLCQKLGGIPLAIELAAARTQVLTAGQILDKLKDPLGFLTA
                     GRRTVATRHQTLRATLQWSYDLLSDDERALFRRLSVFAGGFTLEATEEVGSCDGVARE
                     EVLDLLGGLVDKSLVITEAGGEGTLRYGVLEPVRQFAREKLQESGEEPEARRRHAEHY
                     LGFAEEASPELLGPDQGLWIWRLRSEFANLAEAQAWSLEPGEEEERARVRLRLPAALW
                     RFWTGRRFEEGKAWLLTALDRDTGGYPAVRAKALDGLGYILLFQQDYEKAMAVLQESV
                     AIYKDLGDRSGTALALANLGYAMLHGGYVDGLPAIVAEAEALIAGDLEPHARAYLRQI
                     LATAAIQEGDLRSAAAQFEEGLALSRELGDLRNTAMALFNLGMLDIMQGDLGGATSLE
                     EGSKIALELGDMLGGLYFVWGFGKLSAMRGLPRRAATLWGAADAFRKLMGMAPSRFDL
                     DASGYEEDLAAVRSELGEASFAASWSEGRAMSLEQAIDYALQEPAEVFDVEPTLTPHR
                     PTLERPVDGTGVRVHNLPAARDGFVGRGREMGEVERLLSTTRLLTLTGAGGCGKTRLT
                     LEVARRLTGDYPDGVWLAELASLTEGEQVPGAVAAALGLSTQPDVPFTDALVDFLRSR
                     RTLLVLDNCEHLVEECASLVDTLLGSCEHLRVLATSRETLGVEGETSWAVPSLTLPEP
                     GPEVDPERLGRYEAVELFLKRARSRAPTFGLTPENAVAVADICRKLDGIPLAIELATA
                     RLGVLSVEQISERLDDSLGFLASGDRTTAPRHRTLGATLDWSHELLGDDERALFRRLS
                     VFVGGWTLEAAEAVGAAGGIGGGEVLDLLSSLVDKSLVVTGGGSEVAPRYRMLEPVRQ
                     YALERLGQGAEAEETRRRHATFFMTLAEEARPNLRAELQVEWLHRLETENGNLRGALS
                     WALSTDDLTTAGRLGWALYMFWWIRNYQPEGRRWTEPVFLRRDELPPSLRIRVIVVHG
                     AMAYGQGDIEILYRLSEELTEISREMGGDPLAEANSHLGFGIVAMHRGDLEAAREHQE
                     DALPLFREAGEDGLAAQTHTFFGMALLLGGDHEGARQKFEDGLTLGRSIGDRMSIVIA
                     LFNLAQLALADGDYDAAARGFAEGIAPSQEAGDRGNIAHILEALGMVAGARGEALRAA
                     RLLGASEALISAIGLRGHPYYRPDRDLYERVRGEVRASLGEAAYEIALEEGRNMPTEQ
                     AVAYAMEESAVLEEIPAGPSTSAYTEPAVRRRGDTQRLYGREGEQARLRQMLDEAAGG
                     RGNLAFIGGEAGIGKTALASSVAHEARERGISVWVGHCYEFVATPPYGPWTESGIFDV
                     GSEAVQPPPMLRSGAGTGAASSQAALFERMQAFLTALTRRHPVVLVLEDLHWADPASL
                     ELLRFVSRIVADLGALVVVTYRDDEVTRQLPFYGLLPTLVRESGVRRIRLRRLGESAV
                     REMVAAGYGLSEADEERLVPYLMKLTGGNAFFTVELLRTLEEELVLHQEDERWVLEDP
                     GTVGVPSLVRQVIEGRLERLGEETRRQLAVAAVIGQEVPFDLWRSVGEADDEQLAEAI
                     GHALKARLMEETPGGEKLRFVHALVRETLYRELLPPFRQRWHRRVAEALERTAAPDP"
BASE COUNT          999 a         1930 c         2205 g          979 t
ORIGIN      
        1 ggaggtcatt cggctcgtgg ccaggggaat gagcaaccag cagatcgcag agagcctggt
       61 cttgagcgag cacaccgtcc accgtcacgt ggcaaacgtc ctgcgcaagc tgggggtctc
      121 ctcccgcgcc tctgccgtcg ccgaagcggc ccaactcggc ttgctctgag cccgcactct
      181 ccagctgtcc gaaacagggt caggccgtac cgtcgcgccc tccggccggg aggggccctc
      241 gtcatcccct cgagcctcga ctcgcggccg ctggcttccc gggcgccaga cgacgaacca
      301 gaagagaaag gaagaaggga atggagcctc gcaacgggag tacgggccgt taccgcaaga
      361 gcggagtcag ggcgcccatc atacagggtt ggttcttgtc cgaaggcgtg gcgatcggtg
      421 aggtcgtgct ctccgactac ctttgtagcg tacctcggtg aactgccgct acaatgggaa
      481 gtcggatctt ctgccactgg agaaacggaa ccgtgtctga tgaccgccac aggtagcgtc
      541 tttttgctcc tcggccgctt gcgaagtcgg ggccctctgg agaacctcga tgcctgagga
      601 gcagaaccac tcgccccacg gcgcctctgc cgagaacagg gaagaggctg gctcggcggt
      661 gatctttcac gaacgctccg tggatcgtct gccggacaac ctgccgcccg agcgcaccag
      721 cttcgtcggg cgcggcaggg aggtcgccga gatcgggaag ttgctctccg agcggcagct
      781 cgtgacgctc tgcggtccgg gcggggctgg caagacgcgc ctggctctgg cggtggccag
      841 gagcctcgtc gaccggttcg aggacggggt gtgttgggtt ggactagcct ctatctccga
      901 ccccgagctc ttgcccgaaa cggtagccac ggcgttgggc gtgccagaga cgccggaccg
      961 ctccccaacc gaggcgctcg tggataacct gcagggccgc agggcctttc tgatcctgga
     1021 caactgcgag cacctcatcg aggcgtgtgc ggatctcgcc gacaccctcc tggggtcctg
     1081 cccgtccctg aaactactcg cgacgagcag ggagcccttg cgcgtggcgg gggagaccaa
     1141 cttcatggtg ccctcgctct ccgtccccga ccccgggcgt tcggcttcaa ccgaggagct
     1201 ggcgggttac gaggcggtgc agctcttgct ccagcgcgcc agggacgtca atcccggttt
     1261 caggctgacc gaagagaacg ctgccaccgt ggtgctgcta tgtcagaagc ttggcggcat
     1321 accgctggcg atagagctcg ccgccgccag gacgcaggtg ctcacggccg ggcagatcct
     1381 ggacaagcta aaggaccccc tggggttctt gaccgctggc agacgcacgg tcgccacgcg
     1441 ccaccagacg cttcgggcga ccctgcagtg gagctacgac ctcttgagcg atgacgaacg
     1501 ggcgctgttc cgtcggctct cggtcttcgc cggggggttc acgctcgagg cgaccgagga
     1561 ggtcgggtcc tgcgacggtg tcgcgcgtga ggaggtactc gacctgctcg gtgggctggt
     1621 agacaaatcg ctggtgatta ccgaagccgg cggcgagggc accctgcgct acggggtgct
     1681 cgaaccggta aggcagttcg cgcgcgaaaa gctccaggag agcggggagg agccggaggc
     1741 ccggcgcagg cacgccgaac actacctggg gttcgccgaa gaagccagcc ccgagcttct
     1801 ggggccggat caggggctgt ggatctggcg cctcaggtcc gagtttgcga acctcgccga
     1861 ggcgcaggcc tggtctcttg aaccgggtga ggaggaagag cgagcgcggg tgcgcctgcg
     1921 gctgccggcg gcgttgtggc ggttctggac cgggcggcgt ttcgaagaag gtaaagcgtg
     1981 gctccttacg gcgctggata gggacaccgg tgggtatccc gccgtcaggg cgaaggcgct
     2041 cgatggcctc ggctacatcc tgctcttcca gcaagactac gaaaaggcga tggccgtcct
     2101 gcaggagtcc gtggccatct acaaggatct cggcgaccgt tcgggcaccg ccctggcgct
     2161 cgccaacctc gggtacgcca tgttgcacgg ggggtacgtg gacggcttac cagcgattgt
     2221 ggccgaggcc gaggcgttga tagcgggtga cctcgaaccc cacgcccgtg cctacctgcg
     2281 ccagatcctg gccacggccg ccatccagga aggagatctc cgatccgcgg cggcccagtt
     2341 cgaggagggg ctggccctga gccgcgagct gggcgacctc cgcaacaccg ccatggccct
     2401 cttcaacctg ggtatgctag atatcatgca gggagacctc ggcggggcga cgtcgctgga
     2461 ggagggaagc aagatcgccc tggagctggg ggacatgttg ggcggcctgt acttcgtgtg
     2521 ggggttcggc aagctgagcg cgatgcgcgg tctgcccaga agggccgcaa cgctctgggg
     2581 ggccgccgat gccttcagga agctgatggg catggccccc tccaggttcg accttgacgc
     2641 ctccggatac gaagaggacc tggccgcggt acgctccgag ctgggtgagg cgtcgttcgc
     2701 cgcctcctgg tccgaaggaa gagccatgtc cctcgagcag gccatcgact acgctctgca
     2761 agagccggct gaggtattcg atgtggagcc gacgcttact ccccaccggc ctacccttga
     2821 gcgtcccgtt gatggcacgg gcgtccgtgt tcacaacctg ccggcggcgc gtgacggctt
     2881 cgtgggtagg ggacgcgaga tgggcgaggt cgagcgcctc ctctccacca ccaggctgct
     2941 gacgctaacg ggggcgggcg ggtgcggcaa gacgcgcctg acgttggagg tggcccgccg
     3001 gctcaccggt gactacccgg acggggtctg gctcgcggag ctcgcctctc ttacagaggg
     3061 tgagcaggtg ccgggggcgg tggccgcggc ccttggcctg agcacccagc ccgacgtccc
     3121 gttcaccgac gcgctggtag attttctgcg ctccaggcgg acgctgctgg tcctggacaa
     3181 ctgcgagcac ctcgttgagg aatgcgccag cctcgtcgat acgctcctcg gctcctgcga
     3241 gcacctgcgc gttctggcca ccagcaggga gaccctcggg gtggaggggg agaccagctg
     3301 ggcggtaccc tcactcacct tgcccgaacc ggggcctgag gtcgaccccg agcgtctcgg
     3361 gcgctacgag gcggtggagc tgttcctgaa gcgggcgcgc tccagggcgc cgaccttcgg
     3421 gctgaccccc gagaacgcgg tggccgtcgc ggacatctgc cggaagctgg acggcatacc
     3481 gcttgccata gagctggcga cggcccggct tggtgttctg tcggtggaac agatctcgga
     3541 gaggctggac gactcgctgg gctttctagc ctcgggggac cggacgacgg ccccgcgcca
     3601 caggacgctt ggggcgaccc tggactggag ccacgagcta ctgggagatg acgagcgcgc
     3661 gttgtttcgc cggctgtcgg tcttcgtcgg gggttggacc ctggaggcgg ccgaggccgt
     3721 cggtgccgcg ggaggtatcg gtggcgggga ggtgctggat cttctctcct ctctcgtaga
     3781 taaatcgctc gtcgtgaccg ggggtggttc ggaggtagca ccccgctaca ggatgctgga
     3841 gccggtaaga cagtacgccc tggaacgcct cgggcaaggc gccgaggccg aagagacccg
     3901 ccgccggcac gccacgttct tcatgaccct ggccgaggaa gcgcgcccca acctcagggc
     3961 cgagctacag gtggagtggc tccaccggct ggaaacggag aacggcaacc tgcgcggtgc
     4021 cctctcgtgg gcgctctcta ccgacgacct caccaccgca ggcaggctgg gctgggcgct
     4081 atacatgttc tggtggatac gcaactacca gccggaagga cggcggtgga cggaaccggt
     4141 cttcctgagg agagacgagc tcccgccgtc gttgcgcatc cgggtgatcg tggtacacgg
     4201 ggcgatggct tacgggcagg gcgacatcga gatcctctac aggttgtccg aggagctcac
     4261 ggagatctcc cgggagatgg gcggggaccc gctggcggag gccaactctc acctcggctt
     4321 cgggatcgtg gcgatgcacc gtggagatct cgaggcggct agagagcatc aggaggatgc
     4381 cctgccgctc ttccgcgagg ccggagagga cgggctggcg gcgcagacgc acaccttctt
     4441 cgggatggcg ctgctcctcg gcggcgacca cgaaggcgcc aggcagaagt tcgaggacgg
     4501 gctgaccctg gggcggagca taggcgacag gatgagcatc gttatagcgc tcttcaacct
     4561 ggcgcagttg gccctcgccg atggcgacta cgacgcggcc gccagggggt tcgcggaggg
     4621 catcgcacct tcgcaagagg ccggggaccg gggaaacatc gcccacatcc tggaggccct
     4681 gggtatggta gccggtgcgc ggggcgaggc cctgcgggcc gccaggctgc tgggtgcctc
     4741 ggaggccctg atctccgcca tcggcctgcg gggccacccc tattaccggc ccgaccgcga
     4801 cctctacgag cgcgtcaggg gcgaggtaag ggccagtctc ggcgaggcgg cctacgagat
     4861 tgccctggag gagggacgga acatgcccac cgaacaggcc gtcgcctacg ccatggaaga
     4921 atcggcggtc ctcgaggaga tccccgcagg cccgtccacc tccgcctaca cagagccagc
     4981 ggttcgtcgg aggggggata cgcagcggct gtatggacgc gagggggagc aggcccgact
     5041 tcgtcagatg ctggatgagg cagccggcgg gaggggcaac ctcgccttta tcgggggcga
     5101 ggcgggcatc ggcaagaccg cgctcgcctc ctccgtcgcc cacgaagccc gggagcgcgg
     5161 gattagcgtg tgggtcgggc actgctacga gttcgtcgcg accccgccat acggcccctg
     5221 gaccgagagc gggatcttcg atgtgggttc cgaagccgtt cagcctcccc cgatgctgcg
     5281 ttccggggcg gggacgggag cggcgagcag tcaggccgcg ctcttcgaga gaatgcaggc
     5341 gtttctgacg gcgctcacga gacggcaccc cgtggtcctt gtactcgagg acctgcactg
     5401 ggcggacccc gcaagccttg agctcttgcg tttcgtgtcc cggatcgtgg ccgatctcgg
     5461 ggcgcttgtc gtggtcacct acagggacga cgaggttacc aggcaactgc ccttctacgg
     5521 gctgctcccg accctggtgc gcgagtcagg ggtgcgccgc atcaggctgc gcaggctggg
     5581 cgagagcgcc gtccgcgaga tggtggccgc cggctacggc ctgtccgaag ccgacgagga
     5641 acggctcgtg ccctacctga tgaaactcac cgggggaaac gcattcttta ccgtggagtt
     5701 gctccgtacg cttgaagaag agctcgtcct gcaccaggaa gacgaacgct gggtcctcga
     5761 ggatcccgga accgtcgggg taccctcgct ggtgcgacag gtcatagagg gcaggctgga
     5821 gcggctgggc gaggagacca ggaggcagct agccgtcgcg gcggtgatcg ggcaggaggt
     5881 gcccttcgac ctgtggcgct cggttggcga ggcggacgac gagcagcttg ccgaagcgat
     5941 agggcatgcc ctgaaggcac gcctgatgga ggagactcca ggaggggaga agctgcgctt
     6001 cgtccacgcc ctggtccgcg agacactcta cagggagctg ctgccgccct tccgccagag
     6061 gtggcaccgg cgggtcgcgg aggccctgga gcgtacggcg gcgccggatc ccg
//
LOCUS       DBNGVD010000490         5157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1760970, whole genome shotgun sequence.
ACCESSION   DBNGVD010000490 DBNGVD010000000
VERSION     DBNGVD010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1760970"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..472)
                     /locus_tag="AC16CM_13195"
     CDS             complement(11..472)
                     /locus_tag="AC16CM_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140835.1"
                     /translation="MRRMATILAVAALTVTFTSGVALAAFEDSITGTDHTDIFTGTGK
                     AEQISGLGGGDQINGGGGDDLVEGGKGQDEVADGLGQDTVYGGVGKDNLIGQGGDTSL
                     DRFYAGRGDDTVQSWDSPAVKDVVNCGPGTDKVYADEADVIKDNCERVRVQ"
     gene            683..2680
                     /gene="ligA"
                     /locus_tag="AC16CM_13200"
     CDS             683..2680
                     /gene="ligA"
                     /locus_tag="AC16CM_13200"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3140836.1"
                     /translation="MAEGADVRSRMEDLREQVRYHNRRYHVQDAPAISDAEYDALYNE
                     LEALEAEYPELVTPDSPTQRVGGEPVEGFEEVMHSVPMLSLANARKVEDLREWDARVR
                     RLLGDDESPRYVTERKIDGLAVSLRYENGRFVRGATRGNGTVGEEVTANLSTVRSIPD
                     RLDDGPPPVLEPRGEVYMALEDFERLNRRQEEEGKPPFANPRNAAAGAIRQLDSRITA
                     RRPLKVYLYGIGEGGEGFESHSALLDGLKDYGLRANPHKLHDSIESVVQECLAAASER
                     ESLPYQIDGVVVKVDSREQQLALGSVARAPRWAIAFKFEPLAGRTKLLDVIVNVGRTG
                     ALTPQAVLEPVNVGGVVISRATLHNEDYIKEKGILVGDTVIVERAGDVIPQVVKPVTE
                     ERGGGEYAFEMPARCPVCDEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKTAMDID
                     GLGEKLATRLFDLGFIKDSADVYDLEAGQLIPLEGFGEKSAENLIGAIQKSKEKPFSS
                     VLYALGIRHVGAVTADLIATRFGGEDLLGGVEVEQLSEIKGLGEVVARAVVEYFVLDD
                     NRDLVERLMQRGLDFGRVEGSPSEGPLAGKRVVITGTLEHPRSYYVERLEAAGGSFTS
                     SVSKNTDYVLAGEEAGSKLQKATDLGVPILDHEGFEQLLSS"
     gene            complement(2973..3878)
                     /locus_tag="AC16CM_13205"
     CDS             complement(2973..3878)
                     /locus_tag="AC16CM_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3140837.1"
                     /translation="MDERELLANRFEEHRPRLKALAYRMLGSPSEAEDAVQEAWLRLD
                     RSDSGGVENLGGWLTTVVGRVCLNMLRSRGSKRERVLDEPWDETSDAWMPDPIVDRED
                     GIDPEHQALLAEGIGLALLVVLETLSPAERIAFVLHDTFGVPFDEIAPIVGRSPAASR
                     QLASRARRRVQGEATLPAANASRQREVVDAFLVASRDGDLGALLAVLDPEVVLRADPG
                     AAGERREVHGAHAVAEQARAFSHLGRFSRRALVNGTAGIVSRAPGGPTFSVMGFTVVS
                     GRITEIDILADPERLRPLDLAFLDD"
     gene            4010..4483
                     /locus_tag="AC16CM_13210"
     CDS             4010..4483
                     /locus_tag="AC16CM_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3140838.1"
                     /translation="MQESVVRGRMDNPVMVVPGALQPLLALANSAREVGLSPRTADLV
                     HLRAAQINGCSYCVDMHARDLKKAGETDERLFAVAAWRETPYFSEAERAALALTEAAT
                     RLADRPDPVPDVVFEEAARHYDEPTLAALIIEIALINFWNRTTVAVRQIAGSGPT"
     gene            complement(4559..>5157)
                     /locus_tag="AC16CM_13215"
     CDS             complement(4559..>5157)
                     /locus_tag="AC16CM_13215"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3140839.1"
                     /translation="LVLPPFYYKGVGDDGLFRYFAEVIERIGDERLRLYLYHIPQMTG
                     IHLGLPLISRLIEAYPGMIAGTKDSSGDRERIGTLCREFPDLSVLVGTETLLLETLRN
                     GGDGCISATVNVTSRLARQVHDAHASARDDEAQTLQERLSALRASIETYPVIPALKAL
                     MADMTGDGNWRNLRPPLSNLDAEQRKGLLAEVPLDDLL"
BASE COUNT          910 a         1702 c         1690 g          855 t
ORIGIN      
        1 gagcgcgctc ctactgcacc ctcacccgct cgcagttgtc tttgatgacg tcggcctcgt
       61 cggcgtagac cttgtccgtg ccggggccgc agttcacgac gtccttgacg gcgggtgagt
      121 cccaggactg aacggtatcg tcgccgcggc ccgcgtagaa gcggtcgagg gaggtgtctc
      181 cgccctggcc gatcaggttg tccttgccaa caccgccgta gacggtgtcc tgcccgagcc
      241 cgtcggccac ctcgtcctga cccttaccgc cctcgacgag gtcgtcgccg ccgccgccgt
      301 tgatctggtc gccgccgccg agtccggaga tctgctctgc cttgccggtg ccggtgaaga
      361 tgtcggtgtg gtcggtgcca gtgatggaat cctcgaaggc cgcaagggcc acgccggaag
      421 tgaaggtcac cgtgagggcc gctaccgcca gaatcgttgc catcctcctc atctgcttcc
      481 cctctctcgt tccgactccg gtctacgatt cgtactctag gggggtcccg taacgcctcg
      541 gctacagatg cataaccttt cggccacgtt cttataacgt ttcgataaca atcccccggc
      601 ggagtccgaa aacaggcgat tgcaggacac gtcccgcgca gatagggctt ctcctggaag
      661 cgttcgtgcg tataattcct gcgtggctga aggcgcggac gttcggagca ggatggagga
      721 tctgcgcgag caggtccgct accacaaccg ccgctaccac gtacaggatg cgccggctat
      781 ctccgacgcg gagtacgacg ccctctacaa cgagctcgag gccctggagg ccgagtaccc
      841 cgaactcgtc acgcctgact cgccgaccca gagagtgggc ggggagcccg tggaggggtt
      901 cgaggaggtc atgcactccg tccccatgct ctcactcgcc aacgcccgca aggtggagga
      961 cctgagggag tgggatgcca gggtgcggcg cctgctcggc gatgacgagt cgcctcgtta
     1021 cgttaccgag cggaagatag acggcctcgc cgtctctttg cgctacgaga acgggcgctt
     1081 cgtccgtggc gccacgcgcg gcaacggcac cgtcggcgag gaggttacgg ccaacctctc
     1141 caccgtccgc tccatccccg accgtctcga cgacggcccg ccgccggtgc tcgaaccgcg
     1201 gggcgaagtc tacatggcgc tcgaggactt cgagcggctc aaccggcggc aggaggaaga
     1261 aggcaagcca cccttcgcca acccgcgcaa cgcggcggca ggggccataa ggcagctcga
     1321 ctccaggatc acggccagaa ggccgctcaa ggtctacctc tacggcatcg gcgagggcgg
     1381 cgaaggcttc gaaagccact ccgcgctgct cgacgggctc aaggactacg gcctgcgggc
     1441 caacccgcac aagctacacg actccataga gtccgtagta caggagtgcc tggccgccgc
     1501 cagcgagcgc gagtcactcc cctaccagat agacggcgtc gtggtgaagg tcgattcccg
     1561 cgagcagcaa ctcgccctcg gctccgtggc cagggcccca cggtgggcca tcgccttcaa
     1621 gttcgagccc ctggcggggc gcacgaagct gctcgacgtc atcgtaaacg tgggcaggac
     1681 cggcgcgctg acgccgcagg ccgtgctcga gccggtaaac gtgggcgggg tcgtgatctc
     1741 ccgcgccacc ctccacaacg aggactacat caaggagaag ggcatcctcg tcggcgacac
     1801 cgttatcgtc gaacgggctg gggacgtgat cccccaggtc gtcaaacccg ttaccgagga
     1861 gcgtggcggc ggcgaatacg ctttcgagat gccggcccgc tgccccgtct gtgacgagcc
     1921 ggtctcgcgt cccgagggcg aggcggtcac ccgatgcgtg aacgcccgct gcccagccca
     1981 ggcgctggag cacattattc actggtcctc caaaaccgcc atggacatcg acggcctggg
     2041 cgagaagcta gccacccgtc ttttcgatct cggcttcata aaggactcgg ccgacgtcta
     2101 cgacctggaa gccgggcaac tcatcccgct ggagggcttc ggcgagaaga gcgcggagaa
     2161 cctgatcggg gccatacaga agagcaagga gaagcccttc tcaagcgtcc tctacgccct
     2221 cggcatcagg cacgtcgggg cggtcacggc agacctgatc gccacgcgct tcggcgggga
     2281 agacctgctc ggcggcgtcg aggtcgagca gctgtccgag atcaaggggc tcggggaagt
     2341 cgtcgcccgc gccgtcgtgg agtacttcgt cctggacgac aaccgcgacc tcgtagagcg
     2401 cctcatgcag cgcggcctcg acttcggacg cgtggaaggc agcccctcgg agggaccact
     2461 agccggcaag cgcgtggtca tcacgggaac cctcgagcat ccccgtagct actacgtgga
     2521 gcggctcgag gctgctggcg gcagcttcac ctcctccgtc agcaagaaca cagactacgt
     2581 gctggccgga gaagaggcag gctcgaagct acagaaagcc acagatctag gcgtccccat
     2641 actcgatcac gaagggttcg agcaacttct ttcgtcctga tgtagcttct ggtcttccca
     2701 acgggggttt tcttatgttc aaaagtattg ttatcccgcc aggggtgtac ataagagggt
     2761 ccgggttctg atccgcactt gcacgcgccg ctgcatatct atccgggcca ctagagtaga
     2821 acctggtcta ttgcgaaaga tgctcttccc gagcttccac gcgactccga gagcgcgtct
     2881 gggcaagatc ttgcatccga actttcgaga acgttctgcg acgcgccaag ctgaacagcc
     2941 cgaagccctg cgggcgcaat gtgggttgca actcagtcgt ccaggaacgc cagatcgagc
     3001 gggcggagcc gctctgggtc ggcgagtatg tcgatctcgg tgatcctccc gctcacgacc
     3061 gtgaagccca tcaccgagaa cgtcgggcca ccgggggccc gcgagacgat tcccgcggtc
     3121 ccgttcacca gcgcccgacg tgagaaccgg ccaaggtgcg agaaggccct cgcctgctcg
     3181 gccaccgcgt gcgcgccgtg gacctccctc cgctcgcccg cggcgccggg gtccgcccga
     3241 agcacgacct cggggtccag caccgcgagc agcgccccga ggtcgccgtc tcgcgaggcg
     3301 accaggaacg cgtcaacgac ctccctctga cgggaggcgt tggctgcggg taacgtggct
     3361 tccccttgca cccggcgcct ggcgcggctg gccagctgcc gcgaggcggc cggggagcgc
     3421 cctacgatgg gcgcgatctc gtcgaaggga accccgaagg tatcgtgcaa aacgaacgcg
     3481 atccgctctg cgggagagag cgtttcgagt accacgagca gcgcaagccc tatcccctcg
     3541 gccaggagcg cctggtgctc cgggtcgatc ccgtcttcgc ggtccacgat cgggtctggc
     3601 atccaggcgt cagacgtttc gtcccagggc tcgtcgagaa cccgctcgcg tttagacccg
     3661 cgcgagcgca gcatgttcag gcacaccctg cccacgaccg tggtgagcca cccgcccagg
     3721 ttctccacgc cgccggagtc ggagcggtcg aggcgcaacc aggcctcctg tacggcatct
     3781 tcggcctcgc tcggcgatcc tagcatccgg taggccagcg ccttcaggcg ggggcggtgt
     3841 tcctcgaaac ggttcgcgag cagttcgcgt tcgtccatcc gtcacattcc tccgtcgcgt
     3901 cgggtcatca tagtagagac caatcgaacc cggccgatgt gacgaggagg gcggcccgaa
     3961 gcaaaacgta agcggtcgag taaaggcacg aacggaagaa gggaatcaca tgcaagaatc
     4021 agtcgttcga ggacggatgg acaacccggt gatggtcgtg ccgggtgccc tgcaacccct
     4081 gctggcgctg gcgaactccg cgagggaagt cggcttgtcg ccaaggaccg ccgacctcgt
     4141 ccaccttcgc gcggcccaga tcaacggctg cagctactgc gtcgacatgc acgcccgcga
     4201 cctcaagaag gcgggcgaaa cggacgagcg cctcttcgcg gtggcggcct ggcgggagac
     4261 gccctacttc agcgaggcag agcgcgccgc gctggcgctc accgaggccg ccacccgcct
     4321 cgccgaccgg ccggacccgg ttccagacgt ggtcttcgag gaggccgctc ggcactacga
     4381 cgagccgacg ctcgcggcct tgatcataga gatcgccctg atcaacttct ggaaccgcac
     4441 cacggtggcc gtcaggcaga tagcgggctc gggccccacc tgagcggcgc agtaaggccc
     4501 ccggagctcc tcggtaatcg ttgcctatcc gcgcgagact ctgtctgctg acgggaccct
     4561 agaggaggtc gtcgagaggc acttcggcga ggagcccttt cctctgttct gcatcgaggt
     4621 tggagagtgg tgggcggagg ttgcgccagt ttccgtcgcc ggtcatgtcc gccatgaggg
     4681 ctttgagcgc cgggatcacg gggtaggtct cgatggaggc gcggagcgcg ctcaggcgct
     4741 cttgcagggt ctgggcttcg tcgtctcttg ccgaggcgtg ggcgtcgtga acctggcggg
     4801 ccaggcgtga ggtgacgttg acggtggccg agatacagcc gtcgccgccg ttgcgcagcg
     4861 tttcgagcag gagcgtctcg gttccgacca ggacggagag atctgggaac tcccggcaga
     4921 gggtcccgat gcgttccctg tctccggagc tgtctttcgt gccggcgatc atgcccgggt
     4981 aggcctcgat gaggcgtgag atcaggggca gcccgaggtg tatgcctgtc atctgtggga
     5041 tgtggtagag gtagaggcgc aggcgttcgt cgccgatgcg ctcgatgacc tcggcgaagt
     5101 agcggaacag gccatcatcc cccacgccct tgtaatagaa ggggggcaac acgagaa
//
LOCUS       DBNGVD010000491         1882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5040602, whole genome shotgun sequence.
ACCESSION   DBNGVD010000491 DBNGVD010000000
VERSION     DBNGVD010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5040602"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16CM_13220"
     CDS             <1..354
                     /locus_tag="AC16CM_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140840.1"
                     /translation="FADGNGQTLLITTGDHETGGLTVEATDDPEYPDESGGGQGDPNA
                     NLSVEDGPFRVANSDYEMILDWTTTAHTGADVPLTASGPGGRDLVGNYQNTHVYDAMA
                     DALGIPRAQRSKLAN"
     gene            495..>1882
                     /gene="proS"
                     /locus_tag="AC16CM_13225"
     CDS             495..>1882
                     /gene="proS"
                     /locus_tag="AC16CM_13225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3140841.1"
                     /translation="MTEAVEKLVKKSEDPSRWYLQLVRMAKLADYGPVRGTFAIRPYG
                     FEIWERVQRDLDDRFKATGHKNAYFPLLIPESYLQKEADHVEGFDPELAWVTIGGREE
                     LEERLAIRPTSESIIGAFYRNWIQSYRDLPVLINQWCNVLRWEKVTRPFLRTSEFLWQ
                     EGHTVHATAGEAREETLRMLGVYRDCFYETMAMPVLTGAKSESERFAGAVETFTCEGL
                     MGDGRALQAATSHDLGQNFARAFDITFLDENQDRVHPYQTSWGFSTRTIGGLILVHGD
                     DRGLKLPPKIAPTQAVVVPIWRGKNKGEVRREAEALYGELRDAGLRMEVDLDEEHSPG
                     WKFNEHELRGVPVRVELGPKDIEKGQAVLVRRDTGEKEFVDRRELAGRLNGLMGEIQE
                     NMLHQAETFREENTRRAQSYDDLKEIIAEKRGFVVAPWDGSRETEQKIKEDTKATIRL
                     LPFEREEGEDLVS"
BASE COUNT          390 a          579 c          630 g          283 t
ORIGIN      
        1 ttcgctgacg gtaacggtca gactctgctc atcaccaccg gggatcacga gaccggtggg
       61 ctcaccgttg aagccaccga cgacccggag tacccggatg agtcaggtgg cgggcagggg
      121 gacccgaacg ccaacctctc cgtcgaggac ggaccgttca gggtcgccaa ctccgactac
      181 gagatgatcc tagactggac caccaccgcg cataccggcg cagacgtgcc gctgacggca
      241 agcggcccag gcggacgaga tctggtcggc aactaccaga acacccacgt ctacgacgcc
      301 atggcggatg ccctaggcat cccaagggcc cagcggtcca agctggcaaa ctgattatcg
      361 gctgaccgcc gtagcgcagg ggccgccggg aattatcccg gcggcccctg cgcatctttc
      421 accccgggcc tctagtggga ccagaacgct atactctctt gctggtctct atttcatcag
      481 gagaggagta gcgcatgacc gaagcggtcg agaagctggt caagaagagc gaggacccga
      541 gccggtggta cctgcaactc gtgaggatgg ccaagctcgc agattacggc ccggtgcggg
      601 ggaccttcgc catcaggccc tacgggttcg agatctggga gcgtgtccag cgcgacctcg
      661 acgaccgttt caaggcgacc ggccacaaga acgcctactt tccactgctc atccctgaaa
      721 gctaccttca gaaggaggca gaccacgtcg agggcttcga cccggagctg gcctgggtca
      781 ccatcggcgg gagggaggag ctcgaggagc ggcttgccat ccgtcccacc tccgagagca
      841 ttatcggcgc tttctacagg aactggatcc agagctacag ggatctgccc gtcctgataa
      901 accagtggtg caacgtgttg cgctgggaga aggtgacccg cccgttcttg cggacctccg
      961 agtttctctg gcaggaaggc catacggttc acgcgaccgc cggggaggcg cgggaggaga
     1021 cgctgcggat gctaggcgtc taccgcgact gcttctacga gaccatggcc atgccggtgc
     1081 tgaccggcgc gaagagcgag tcggagcgat tcgcgggggc ggtcgagacg ttcacgtgtg
     1141 aggggcttat gggcgacgga cgcgccctgc aggcggcgac gagccacgac ctcgggcaga
     1201 actttgcccg agccttcgac atcaccttcc tcgacgagaa ccaggacagg gtccatcctt
     1261 accagacatc gtggggcttc tcgacacgaa ccataggggg cctcatcctg gtccacggcg
     1321 acgacagggg cctcaagctt ccgccaaaga tcgcaccaac ccaggcggtg gtggtaccga
     1381 tctggcgggg caagaacaag ggcgaggtcc gccgcgaggc ggaggccctg tacggcgagc
     1441 ttagagatgc cggccttcgt atggaggtgg acctggacga ggagcactcg ccaggctgga
     1501 agttcaacga gcacgagctc aggggcgttc cggtgcgcgt cgagctcggt cccaaagaca
     1561 tcgagaaggg ccaggccgtg ctggtgcgcc gcgacaccgg cgagaaggag ttcgtggacc
     1621 gaagggagct ggccgggagg ctcaacggcc tgatggggga gatccaggag aacatgctcc
     1681 accaggccga gaccttccgc gaggagaaca cccgtcgggc gcagagctac gacgacttga
     1741 aggagatcat cgccgaaaaa cgtggcttcg tcgtcgcccc ctgggacggt agcagggaga
     1801 cggagcagaa gatcaaagag gacaccaagg ccacgatccg gctcctcccc ttcgagcgcg
     1861 aggagggcga ggacctcgtc tc
//
LOCUS       DBNGVD010000492         6652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_122188, whole genome shotgun sequence.
ACCESSION   DBNGVD010000492 DBNGVD010000000
VERSION     DBNGVD010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_122188"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..3477)
                     /locus_tag="AC16CM_13230"
     CDS             complement(205..3477)
                     /locus_tag="AC16CM_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3140842.1"
                     /translation="MGSLDHDGAEVGREIPREAPTHANAGTVVGDGWLRSVMESSSEI
                     IKVVVPDGTLRYVNSAFGRVFGYDPEEVVGSMNVLEQVHPDDLPRILDATEKALAEGG
                     VARNRVEYRFRHADGSWRWIEGIGTYLQDDPEVGGVVVNARDVTERKEAEAALAESEK
                     RFRSQYENLPVPTFTWRRDPQDFVLVDHNDAARRLAGADKLPGSVATDLFSEMPQMME
                     MIGRCSSKQITLKREMPWRLPGSAGERRIVVTCVPVDPDLVMVHTEDVTERATAEGRL
                     RFQAQLLGAVGEAVIAIDADRKVVYWNGAAEELYGYTAEEVLGRRLMEHLVPEHHQSH
                     AEEIMAEVRSGADWKGEFVVKRRDGAPLTVEITNSPIFGEDGTQVGVVGVLRNVTERR
                     ETEAKLRESEERFRGIFEDAPIGVAMVSLPSSPGADRRYLRVNRALCEMLGYPEEVLL
                     SMTTSEVTHPEDVQKSRDRMERMLEEDGSRYTLEKRYIRSDGRVVWALLNVSLARDSE
                     GRHSHFVAQFQDVTQRKEAEEALRRSEERYRTVVERQTELVCRFLPDLTITFVNEAYA
                     AYHGRRPESLVGHTFLDRVHEEDHAYYYEGLTRLSPQSPTSTVEERFFTPDGVRWLQW
                     TDTAIFDGDGRVIEYQSVGRDVTERRELEERLEHQVLHDHLTGLPNRRLLVDRIEQAL
                     ARTGSRGESRAAVLFMDLDNFKHVNDSLGHEAGDLLLTVVPRRIKECLGPQDTLARFG
                     GDEFVVLIEDVETTDDAAKVAGRIAESFEIPFRLDGRDLYARASIGIVFGDASTRSPE
                     DLLRNADTAMYRAKEAGGGYEIFDPDMHEHFVDRLELENGLRRAVEQDEFVVHYQPIV
                     SLDDGGVLAMEALVRWEHPERGLLHPDEFVPVAEKNGLMVPIGEKVLEEACRRARAWQ
                     DERPQAPVTSVNLSAWQLGRRDLAEVIEKTLGKAGLEARFLSLDVTETVYVKTLSRNT
                     AALSRLRALGVGISIDDFGMGYSSLAYLKRLSADALKVDRSFVGDLGEAAEDAAILQT
                     IIELAHTLGMKVVAEGVENEGQASLLRAMGCDFGQGFYFSKPLPPEEATAFLNATHRE
                     PA"
     gene            complement(3593..3665)
                     /locus_tag="AC16CM_13235"
     tRNA            complement(3593..3665)
                     /locus_tag="AC16CM_13235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Arg,seq:ccg)
     gene            3780..4499
                     /locus_tag="AC16CM_13240"
     CDS             3780..4499
                     /locus_tag="AC16CM_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3140843.1"
                     /translation="MYAVLSDIHGNLDALEAVLRDVPEEVDTIFCLGDVIGYGANPNE
                     CCDIVRELGMPTISGNHDLAVTDLSTDLNWFNPTAAAAVLWTREHLSGENADFLRSRP
                     RMLQSEGALFVHGSVRDPDEYIINRVSAEENLAILRSDYPGVGICYFGHTHVKAVAPS
                     PNGAMIGGHTLDLQTGGPYLVNPGSVGQPRDGDAFASYVISDGEQLLYRFVEYDIKRA
                     QDKIRAAGLPNMLADRLAVGR"
     gene            4512..4898
                     /locus_tag="AC16CM_13245"
     CDS             4512..4898
                     /locus_tag="AC16CM_13245"
                     /inference="COORDINATES: protein motif:HMM:NF021486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HIR3140844.1"
                     /translation="MNPAFERLFSVEEANALLPTLRDILDDVSLHRDALREKAPHMEP
                     ILKASAENGGGKAGSEYGLETYRLYLAVERIRELGVVVKDLDMGLLDFPHEREGRIVF
                     LCWHPPEERVAYWHDIDAGYAGRQPI"
     gene            4909..5349
                     /gene="dtd"
                     /locus_tag="AC16CM_13250"
     CDS             4909..5349
                     /gene="dtd"
                     /locus_tag="AC16CM_13250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3140845.1"
                     /translation="MRIVLQRVREASVTVAGEKISEIGPGFLLLVGVADGDGEPEADW
                     LAEKISGLRIMPDENGKMDLSVKDTGGAVLAVSQFTLLADTRKGKRPSFVKAAPPERA
                     TRLFDYFCDQVHAAGVTRVEKGRFGAMMDVHLVNDGPVTIVLER"
     gene            complement(5414..>6652)
                     /locus_tag="AC16CM_13255"
     CDS             complement(5414..>6652)
                     /locus_tag="AC16CM_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3140846.1"
                     /translation="GGPGFEGEVRGYLGSLGIGGGDAGRPMKDLSGGQRKLAVLASCL
                     VRRPDLLLLDEPEAHLDAGRRELLEAIVRAFDGAVVVVSHDRYLLDETVTEIAELENG
                     AITMWPGNYSAYSVARELRIKRQQQLYVTQQKEIARLEEAIRRFKLWASLVPNERHIK
                     QARNKQRQIDQMDKVDRPALERRKINLDLRGRVRGGKKVIELREVSIAFEDDPILIGV
                     DLTVERGERLGIVGPNGAGKSVLAKILAGVLPPTEGERWAGPSIDIGYLAQNDEPPPG
                     ATPLGLVRDARPMYEGDAVKLLGRFLFRYGQVREPVTALSGGERTRLQLLLIMLREPN
                     CLVLDEPTNHLDIDSLEVLEAELERFDGTVIFVSHDRYFLDRIADGIVAVGDGEAVRH
                     TGGYSDWSRSVLEQVDHQPL"
BASE COUNT         1138 a         2233 c         2058 g         1223 t
ORIGIN      
        1 ggagaaggca agggagctct gggagacgag cgccgcgctg gttggtctcg agccggcgga
       61 gacaccgcta cgcgccacga ccgcagcgtc cggaggctcc taggtgagct tgtcctattc
      121 gtcgagcggg accgatagga ttagaacctg cgacaaacgg cctcaggatg gcatctaccg
      181 tcgtaggccg tcgacattac cgtgtcacgc tggttctcgg tgagtagcgt tcaggaaggc
      241 cgtcgcctcc tctggaggca gcggtttgga gaaatagaag ccctgaccga aatcgcagcc
      301 catcgccctg agcagtgagg cctgaccttc gttctccacg ccctccgcca cgaccttcat
      361 gccgagcgta tgggccagct ctatgatcgt ctgtaagatc gctgcgtcct cggccgcctc
      421 accgaggtcg ccaacgaagg acctgtccac cttcaacgcg tcggcggaca ggcgcttgag
      481 ataggcgagc gatgagtatc ccatgccgaa atcgtctatg gagatgccca cgccaagggc
      541 tctcaggcgg ctgagggccg ccgtgttccg gctcagcgtc ttcacgtaga cggtctccgt
      601 cacatcgagg ctcaggaatc ttgcctccaa cccggccttt ccgagcgtct tctcgatgac
      661 ttcggcaagg tcgcgacgcc cgagttgcca ggccgagagg ttgacggacg tgacgggagc
      721 ttgcgggcgt tcgtcctgcc aggctctggc cctccggcag gcctcctcga gcaccttctc
      781 cccgatcggc accatcaggc cgttcttctc ggccacgggt acgaactcgt ccgggtgtag
      841 caggccgcgc tcggggtgct cccacctcac cagtgcctcc atggcgagga ccccgccgtc
      901 gtcgaggctc acgatcggct ggtagtggac gacgaactcg tcctgctcca cggcccgcct
      961 caggccgttc tcgagctcca gccggtccac gaaatgttcg tgcatgtcag ggtcgaatat
     1021 ctcgtagccc ccacctgcct ccttggcccg gtacatggcc gtgtcggcgt tcctgaggag
     1081 gtcctcggga gatctggtac tggcatcgcc aaagactatg cctatgctgg ccctggcgta
     1141 cagatcccta ccatccagcc ggaacgggat ctcgaatgac tcggcgatcc ttccggcaac
     1201 cttcgcggca tcatcggtgg tctctacatc ctctatcagc acgacgaact cgtcaccccc
     1261 gaaccgggcc agggtatcct ggggcccgag gcactccttg atacgccggg gcacgaccgt
     1321 taggagcagg tcgccggcct cgtgaccgag ggagtcgttg acgtgcttga agttgtcgag
     1381 gtccatgaaa agcaccgccg cccggctctc cccgcggctc cccgtcctgg ccagggcttg
     1441 ctctatgcgg tcgacgagca ggcggcggtt ggggaggccg gtcaggtggt cgtgtagtac
     1501 ctggtgctcg aggcgctcct ccagttccct tctctcagtg acgtccctac ctacggactg
     1561 gtactcgatc acacgcccgt ccccgtcgaa gatggcagtg tcggtccact gcagccagcg
     1621 cacgccgtcg ggcgtaaaga agcgctcctc caccgtgctg gtcgggctct gtgggctcag
     1681 gcgcgtcaga ccttcgtaat agtaggcatg gtcctcctcg tgtacgcggt ccaggaaagt
     1741 atggccgacc agggactcgg gtctacgccc gtgataggcg gcgtaggcct cgttgacgaa
     1801 cgtgatggtc aggtccggca ggaagcggca gaccagctcc gtctgcctct cgaccacggt
     1861 acggtagcgt tcctcgcttc gccggagtgc ctcctccgcc tccttgcgct gcgtaacgtc
     1921 ctggaactgg gcgacgaagt ggctgtgtcg tccctccgag tcccgggcca gcgagacgtt
     1981 tagcaacgcc cacaccaccc gcccgtccga gcggatgtaa cgcttctcca gggtgtacct
     2041 ggagccgtcc tcctcgagca tccgctccat gcgatcccgg ctcttctgta cgtcctccgg
     2101 gtgggtcacc tccgaggtgg tcatggacag gagcacctcc tccgggtagc cgagcatctc
     2161 gcagagggcc cggttgaccc tgaggtaacg cctgtcggcc ccgggtgatg acggcaggct
     2221 gaccatggcc acgccgatgg gggcgtcctc gaagatgccc ctgaaacgct cctcgctctc
     2281 cctgagcttc gcctcggtct ccctccgctc cgtaacgttt ctcaggaccc ccaccacccc
     2341 gacctgcgta ccgtcttcgc cgaagatggg cgagttggtg atctcgaccg tcaagggggc
     2401 tccgtccctg cgcttgacga cgaactcgcc cttccagtcc gcccccgacc gcacctccgc
     2461 catgatctcc tccgcatggc tctgatgatg ctctggtacc aggtgctcca tgagccttcg
     2521 gccaagcacc tcctcggcgg tatagccgta cagctcctcc gcggccccgt tccagtagac
     2581 gaccttccgg tcggcgtcga tggcgatcac ggcctctccc acggcaccaa gcagctgcgc
     2641 ctggaagcgg agccgcccct ctgccgtcgc ccgctcggtc acatcctcgg tgtggaccat
     2701 taccaggtcc gggtcaaccg gcacgcatgt gaccacgatg cgcctctcac ctgcgctccc
     2761 aggaagcctc cacggcatct cccgcttcag ggtgatctgc ttgctcgagc agcggccgat
     2821 catctccatc atttgcggca tctccgagaa gagatctgta gccacggagc caggcagttt
     2881 atctgcgccg gcgaggcggc gggcggcgtc gttatgatcg acgagcacga aatcttgcgg
     2941 gtcccgtcgc caggtgaagg tgggcacggg caggttctcg tactgcgacc tgaaccgctt
     3001 ttcgctctcc gcgagtgcgg cctcggcctc cttgcgctcg gtcacgtccc tggcgttcac
     3061 caccacgccc ccgacctcgg ggtcgtcctg caggtaggtg cctatcccct ctatccacct
     3121 ccaggatccg tcggcgtgtc ggaagcggta ctcgaccctg ttcctggcca cacccccctc
     3181 ggcgagggcc ttctcggtgg cgtcaaggat tcggggcagg tcgtccgggt ggacttgctc
     3241 gagcacgttc atggagccga cgacctcttc ggggtcgtaa ccgaaaaccc gcccgaaggc
     3301 ggagtttacg tacctgaggg tgccgtcggg aacgaccacc ttgatgatct ccgaggagct
     3361 ctccatgacg gagcgaagcc acccatctcc cacgaccgtc cccgcgttgg catgcgttgg
     3421 tgcctcgcga ggaatttctc tacctacctc ggccccatcg tgatccaggg atcccaattc
     3481 gccttctcca cagctgatcc cgtcaacgag cgaagtatac agaccgagag tggcagacgc
     3541 ctgtcccgaa gcatggccgg gcaagccttg agaatgacgg actctcgtag agcgggcctg
     3601 gcaggattcg aacctgcgac acacggcccc ggaaaccgtt gctctatccc ctgagcaaca
     3661 ggcccgcgct ggtgcgggga ccgccagggc gatgttaccg taacgacggc ggggcttcaa
     3721 cggacagggg gccgattgaa atcgctcacc caacggttaa actacgccgc actctatata
     3781 tgtacgcggt actctcggac atccatggta acctcgacgc cctcgaagcc gtcctgcggg
     3841 acgtgcccga agaggttgat acgatcttct gcctgggcga cgtcatagga tacggggcaa
     3901 acccgaacga gtgctgcgac atcgtgcgcg agctggggat gccgacgatc tcgggcaacc
     3961 acgacctggc cgttactgat ctcagtacgg acctgaactg gttcaacccg acggcggcgg
     4021 cagccgtcct gtggacccgc gagcatctaa gcggggagaa cgccgacttt ctgcgctcga
     4081 ggccgcgcat gctgcagagt gagggcgcgc tcttcgtgca cggctccgtt cgagaccccg
     4141 acgagtacat catcaacagg gtctccgccg aggagaacct ggccatcttg aggagcgatt
     4201 atcccggggt gggcatttgt tatttcggcc acacgcacgt caaggccgtg gcaccctccc
     4261 ccaacggcgc gatgatcggg ggacataccc tggacctcca gacgggtgga ccgtatctgg
     4321 tcaaccccgg ctccgtcggc cagcctcgcg atggggacgc tttcgcctcc tatgtgatca
     4381 gcgacggaga gcagcttctc taccgctttg tggaatacga catcaagcgg gcgcaggaca
     4441 agatccgggc agcgggcctc cccaacatgc tggcagaccg cctggccgtc ggccgctagg
     4501 ccgctcccga catgaacccc gccttcgaga ggctcttctc cgtagaggaa gctaacgccc
     4561 tcctgccaac gctgcgggat atcctggacg acgtatccct ccaccgcgat gccctgcgcg
     4621 agaaggcccc gcacatggaa cccatcctga aggcatcagc ggagaacggg ggcggaaagg
     4681 ccggctccga gtacggcctg gagacgtaca ggctctacct ggccgtggaa cgcatcaggg
     4741 agctaggcgt cgtcgtcaag gaccttgaca tgggcctgct cgatttcccc cacgaacgcg
     4801 agggacgcat agtcttcctc tgctggcacc ccccggaaga gcgagtggca tactggcacg
     4861 atatcgacgc cggctacgcc ggacgccaac ccatataggg cagcggcctt gcgcatagtc
     4921 ctgcaacgcg tcagagaagc ctccgtgacc gtggccggag agaagatctc cgagataggc
     4981 ccgggtttcc tcctgctcgt cggcgtcgca gacggggatg gagagccgga agccgactgg
     5041 ctggcagaga agatatcggg gctcaggatc atgcccgacg aaaacgggaa gatggacctg
     5101 agcgtcaagg ataccggcgg ggcggtcctg gccgtctcac agttcaccct gctggcagac
     5161 acccgcaagg gcaaacgacc gagcttcgtc aaagccgccc ccccggagag agccacacgc
     5221 ctcttcgact acttctgcga ccaggtacac gcggcaggcg tgacgagggt cgagaaaggc
     5281 cgtttcggcg cgatgatgga cgtacacctg gtcaacgacg gacccgtcac gatcgttctg
     5341 gagaggtaga ggcgggccac ccaggacccg gttcggtgta tcgtgtctct gctgggcggt
     5401 tcgtgaaccg cccctacagg ggttgatgat cgacctgctc tagcaccgat ctgctccaat
     5461 cgctgtagcc gcccgtgtga cggacggctt ccccgtcacc taccgctact atgccgtcgg
     5521 cgatacggtc gaggaagtag cggtcgtggg agacgaagat gaccgttcca tcgaagcgtt
     5581 ccagctcggc ttcgaggacc tccagcgagt cgatgtcgag gtggtttgtc ggctcgtcga
     5641 ggacgaggca gttcggttcg cggagcatga tcaggagcag ctgcaatcgg gtccgctctc
     5701 ccccgctcag ggcggtgacg ggctccctga cctgcccgta gcggaacagg aagcgcccga
     5761 gcagcttgac ggcgtccccc tcgtacatcg gtctggcgtc gcgcacgagg cccagcggtg
     5821 tggcgcccgg cggcggctcg tcgttctgcg cgaggtaccc gatatctatg gaagggcctg
     5881 cccagcgctc gccctccgtt ggtgggagta cgccggccag gatcttggcg agcacgctct
     5941 tgcccgctcc gttgggcccg acgatgccga gacgctcgcc gcgctccacg gtcaggtcga
     6001 cgccgataag gattgggtcg tcttcgaagg ctatgctcac ctctcggagc tctatgacct
     6061 tcttgcctcc cctgaccctg ccgcggaggt ccaggttgat cttgcgccgc tccagcgcgg
     6121 ggcgatcaac cttgtccatc tggtcgatct ggcgttgttt gttgcgcgcc tgcttgatgt
     6181 ggcgttcgtt cgggacgagg ctggcccaga gtttgaagcg ccggatcgcc tcctccagcc
     6241 gcgctatctc tttttgctgc gtgacgtaga gttgctgctg acgcttgatc ctgagctcgc
     6301 gggccaccga gtaggccgag tagttgcccg gccacatcgt aatagcgccg ttctccaact
     6361 cggcgatctc cgtcaccgtc tcgtcgagca ggtagcggtc gtgggagacc acaacgacgg
     6421 cgccatcgaa ggcgcgcact atggcctcca acaactcccg gcgtccggcg tcgaggtggg
     6481 cctccggctc atcgaggagg agcaagtctg gacgccggac gaggcaggag gcaagcaccg
     6541 ccagcttgcg ctgcccgccg ctcaggtctt tcatcggacg cccggcatcc cccccaccga
     6601 tgccaagaga gcccaggtag ccgcgaacct cgccctcgaa gccgggcccg cc
//
LOCUS       DBNGVD010000493         2091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_852158, whole genome shotgun sequence.
ACCESSION   DBNGVD010000493 DBNGVD010000000
VERSION     DBNGVD010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_852158"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..324
                     /gene="moaD"
                     /locus_tag="AC16CM_13260"
     CDS             82..324
                     /gene="moaD"
                     /locus_tag="AC16CM_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIR3140847.1"
                     /translation="MIKVLFFGAAADRAGTRETELDVREGATLAEVWTLIVERHADLA
                     PMGDTLAFAVNGEYARGEDTVSPGDEVAVLPPVSGG"
     gene            325..768
                     /locus_tag="AC16CM_13265"
     CDS             325..768
                     /locus_tag="AC16CM_13265"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR3140848.1"
                     /translation="MFAIVEGAIDVGALVADAQRPDCGAIATFVGTTRVDESGGASVE
                     YLEYEAYRPMADRKLAEIGAEIRARWDVGHVSIVHRLGRVDPGEASVTIVVASPRRGP
                     AFEASRYAIERIKEVVPIWKREVWSDGYVWVGSQVGTRGSASERR"
     gene            complement(844..1218)
                     /locus_tag="AC16CM_13270"
     CDS             complement(844..1218)
                     /locus_tag="AC16CM_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR3140849.1"
                     /translation="MSEANGEAEQGQTEVREHLANERTLLSWVRTGISLISLGLVVER
                     AGALAEAANFKVGSTSGSEFFGLGLALLGAFALILGTIQFLRNRRSIASGDFESSVTV
                     YLIIVAGSLAFAGAFVFYVLFS"
     gene            1401..>2091
                     /locus_tag="AC16CM_13275"
     CDS             1401..>2091
                     /locus_tag="AC16CM_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR3140850.1"
                     /translation="MDRRVGLEQEFFLVEESGRPSERADEFLELCRAMTEAESGSAAC
                     FAPEFVLGLVEVNTAPVYALSGLEREYARNLETALRTAGSLGLRLYPLGTYPLPLQPA
                     ARDGLDYRVQVGTVGPERFVDAGRCAGTHLHLELQPGTVDATAAISATASEGARAEAL
                     NIYNLATALDPALIALTRSCPFFEGRATGLAVRTVHYRGNAHFGWEGVYTDLPQVGAL
                     LPYADDSQHLIK"
BASE COUNT          348 a          672 c          735 g          336 t
ORIGIN      
        1 gagttctccg tcatccccca ttcgctcaaa gtgctggtcg ggccggatta cgtgcccgag
       61 cccggcggat agaatagtgc catgatcaag gtcctgttct ttggcgccgc ggccgaccgc
      121 gcggggaccc gcgagacgga gctggacgtt cgggaaggcg caacgctggc tgaggtatgg
      181 acgctgatcg tggaacgcca cgcagacctc gcccccatgg gcgacacgct ggccttcgcg
      241 gtgaacggcg agtacgcgcg cggggaagac accgtctccc ccggcgacga ggtggcggtc
      301 cttccgcccg tctcgggcgg ttaggtgttc gccatcgtcg agggggctat cgacgtgggc
      361 gccctggtcg ccgatgccca gaggccagat tgcggggcca tcgccacgtt cgtgggcacg
      421 acgagggtcg acgagtcggg tggcgcatcc gtggagtacc tggagtacga ggcataccgg
      481 cccatggcag acagaaagct ggcggagatc ggggcggaga tcagggcgcg ctgggacgtg
      541 gggcacgtct ccatcgtgca ccgcctgggc agggtggacc cgggtgaggc cagcgtcacc
      601 atagtcgtcg cctccccgcg gcgcgggccc gccttcgagg cgagccgcta cgctatcgag
      661 cgcatcaaag aggtcgtgcc aatctggaag cgcgaggtct ggtcagacgg gtatgtgtgg
      721 gtggggagcc aggtcgggac gagggggtcg gcctccgaga ggcggtgaat cgaggctaca
      781 ggtttagggg ctccgctgat actacagggc gtgccttatg ccgaccagcc acgccaaaac
      841 acctcaagaa aacaatacgt agaagacgaa ggcgccggcg aaggccaggc tgccggcaac
      901 gattatcagg tagacggtca cggacgattc gaagtcgccg ctcgcgatgc tacgccggtt
      961 gcggaggaac tggatggtac ccaggatcag ggcgaaggcg ccgagcaggg ccaggccgag
     1021 cccgaagaat tccgagccgc tcgtggagcc gaccttgaag ttggccgcct ccgccagggc
     1081 gccggcccgc tcgacgacga ggccgagcga gatcaggctg atccccgtcc tcacccagga
     1141 gagcagcgta cgttcgttgg cgaggtgctc ccggacctcg gtctgaccct gctcggcttc
     1201 cccgttcgct tcgctcactc ggtcggacct ccctctgcag tagattacac gctgtagcac
     1261 cggagcgttc tggggctccc agcttttgtg ccgtggggcc aatgtccggc cctggttccc
     1321 tggggtagag tttttccctc ggaacgtgga caataggcat ctgccgttgg gagaccagat
     1381 cgaacgaagg gaacaggcta ttggataggc gcgtcgggct cgagcaggag ttctttctgg
     1441 tcgaggagtc ggggcgtccg tcagagcggg ccgacgagtt cctggagttg tgcagggcca
     1501 tgaccgaggc ggagtccggc tcggcggcgt gcttcgcccc cgagttcgtg cttggcctgg
     1561 tggaggtcaa caccgccccc gtctacgccc tctccgggct ggagcgcgag tacgctcgca
     1621 acctggagac ggcccttcgc acggccgggt ccctggggct caggctgtac ccgctcggca
     1681 cctacccgct gcccctgcag cccgcggccc gagacgggct ggactacagg gtacaggttg
     1741 gcacggtcgg gcccgagagg ttcgtcgacg ccggcaggtg cgccggcacg cacctgcacc
     1801 tggaactgca gccgggaacg gtagatgcga cggcggccat ctccgccacc gcatcagagg
     1861 gcgcgcgtgc cgaggccctc aacatctaca acctggccac cgccctcgac cccgccctga
     1921 tcgcgctgac ccgttcgtgt cctttcttcg aggggagggc aacggggctg gccgtccgca
     1981 cggttcacta caggggcaac gcccacttcg ggtgggaggg ggtctacacc gacctgcccc
     2041 aggtcggcgc cctgctcccc tacgccgacg actcgcaaca cctcatcaag c
//
LOCUS       DBNGVD010000494         6160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5042789, whole genome shotgun sequence.
ACCESSION   DBNGVD010000494 DBNGVD010000000
VERSION     DBNGVD010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5042789"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16CM_13280"
     CDS             complement(<1..632)
                     /locus_tag="AC16CM_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3140851.1"
                     /translation="MIESQIESQNFIGGEWVPADSGETSDIVNPSQPSEVLGTTPRSD
                     RAETERAIEAASEALPDWKETLPAARGAVLMEAANILESREDELARLMALEAGKPMKE
                     ARMEVGRAASIFRYYGSEGWRLEGINPPSARPGVTHASMREPLGVVGLITPWNFPLAI
                     PAWKMAPALICGNTIVIKPAGNASLNAAALVRILTEAGLPEGVLNMVTGPG"
     gene            complement(701..1516)
                     /locus_tag="AC16CM_13285"
     CDS             complement(701..1516)
                     /locus_tag="AC16CM_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3140852.1"
                     /translation="MYLSRHEAAGGPRWALDGRYLPPEFTLRRLLAMKATEVRSFLEE
                     QAPEAAADDPLLPPVEAEQEVWASGVTYLQSRDAREMESEDADAYERVYVAERPELFF
                     KAPGWRVVGHGHPVRVREDSHWNVPEPELVVVVNSGMEVVGYTAGNDVSSRDIEGENP
                     LYLPQAKVYDGSCSLGPGIVLCGPEAMLELRIELEIRRGGESVFRGEVDTSQMKRSPE
                     ELAEYLGRELAFPSGAFLMTGTGIVPEDDFSLGAGDAVSISVGELSLQNEVGP"
     gene            complement(1579..2502)
                     /locus_tag="AC16CM_13290"
     CDS             complement(1579..2502)
                     /locus_tag="AC16CM_13290"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3140853.1"
                     /translation="MELRQVETFRAVARELSFSRAAAELGYVQSSVSTQVAALERELG
                     VPLFDRLGRRIALTEAGAVMLDYAERLLGLAREAGEAVADAGVGRGEISGSLRVSAPE
                     TLLTYRLPGLLTAFHEAHPRVRVSLHPTVVGRLVGSTRRALEEGRVDVAFVLDVPSVG
                     IEGLSVELLREERVSVVAPPDHALATSDLVAPPDLGGETVLLPEAPQSGCAYRGQFER
                     QFAEGGVVPREMLEFQSIEAIKRCVSAGMGVTVLPTVAVGAELSAGDLSELGWKEPIL
                     VGTLMAWNGARWASPALRAFLETAREVLSLS"
     gene            2627..3880
                     /locus_tag="AC16CM_13295"
     CDS             2627..3880
                     /locus_tag="AC16CM_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3140854.1"
                     /translation="MQKDIRVEGGGAPGSRLPAMALLFGGALGISAAFGMLLLLPLYV
                     QELGGNEGDFGVIMASATFTAALAIGLVIRYPEALRPNVLLALAILAYGLGAAGAALV
                     RDGWLPLVGVGLVLGTAWAVVYAASPMVMSGMVTDRERATYFGYLTGTQQLGIGVGPV
                     VAGSLAEGGLGFRGTFLVAAVLCVLAAVLVLAVGILAPDAGGSVPGSDTGPVVSFRAS
                     MGEILRSEGAFWLVMILLFACLFTTLTSFQTTFARAQDLDFSVFYLSYTAAVILSRFG
                     IAGFAARYDTRLVIAVAVSLMALSVASFLFVGSNAVLYGLASAAVGLGYGLALPSAQA
                     QAVNVSEESVRPRVLPLAGLLFETMILAFPLLAGWVISNLGYGAVFVLLVVLCLLQAG
                     LAWERYVRARHQASGNRLQQEKPET"
     gene            3937..5022
                     /locus_tag="AC16CM_13300"
     CDS             3937..5022
                     /locus_tag="AC16CM_13300"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR3140855.1"
                     /translation="MTTTERYRIASIPGDGIGAEVLPEGRRVLEAAARAYGFGLHFDE
                     FDFASCDYYLEHGTMLPAGWKEQLGGYDALFFGAVGWPEKVPDHISLWGSLVQFRRQF
                     DQYVNRRPVRLMPGVTSPLRDPGDIDFVIVRENTEGEYSEVGGRMFEGTEREVVVQET
                     FMTRTGVDRILSYAFELASEREAGHLTSATKSNGISISMPYWDERVREMSTSYPQVSW
                     DQFHIDNLCAQLILRPDFFDVVVGSNLFGDILSDLGPALTGTIGIAPSANINPEKRFP
                     SLFEPVHGSAPDIAGRGIANPVGAIWAASMLLEHVGQPEAAAAVVAAIEHAMGQKELR
                     TPDLGGTATTEECGKAVADTLSSKSVE"
     gene            5184..5786
                     /locus_tag="AC16CM_13305"
     CDS             5184..5786
                     /locus_tag="AC16CM_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140856.1"
                     /translation="MGIKERRERQRREVRGGILSAAREIAAGEGWRAVTIRKIAERIE
                     YSPPVIYEYFGSKDEILLELVRLGYAEQEGMLESVVAKAKSPEEALLGIADAWMDFAF
                     DSPDLYQAMYGLGGAPFAAETRGEGEKIGDLVGNVLEALQQEHGNETGDIEGKVTLLW
                     STVHGLVALTMVSRMPGGEVQARRLARQAAHDYIAAWQTG"
     gene            5926..>6160
                     /locus_tag="AC16CM_13310"
     CDS             5926..>6160
                     /locus_tag="AC16CM_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3140857.1"
                     /translation="MTNGNGTAGTEVVMQHKGGPEALEVVRRGSVSDPRAGEAVVRVE
                     AAGVSFAEVQMLRGRYFSQPKFPFVPGYDLVGRV"
BASE COUNT         1060 a         1996 c         1982 g         1122 t
ORIGIN      
        1 cccggtcccg tcaccatgtt caggaccccc tcgggaaggc ccgcctcggt caggatgcgc
       61 acgagggcgg cggcgttgag ggaggcattg ccagccggct ttatcacgat ggtgttgccg
      121 cagatcaggg ccggggccat cttccaggcg gggatggcga gcgggaagtt ccacggcgtt
      181 atgaggccga cgaccccgag cggctcacgc atggaggcgt gggtgacgcc gggcctggcc
      241 gagggcgggt tgatgccctc caggcgccag ccctcggagc cgtagtagcg gaagatgctt
      301 gcggcgcgtc ccacctccat gcgggcttcc ttcatcggct tgccagcctc gagcgccatc
      361 agccgcgcca gctcgtcctc tcgcgactcc aggatgtttg cggcctccat caacacggcg
      421 ccgcgggccg ccggcagcgt ctccttccag tcgggaaggg cctcggaggc ggcctctatg
      481 gccctctcgg tctccgccct atcagacctc ggggtggtcc cgagaacctc cgacggttgg
      541 gatgggttca ctatgtccga ggtctcaccg gagtcagcgg ggacccactc gccaccgatg
      601 aagttctggg attctatctg cgattcgatc aagagtttcc tcctagcaag acgcctagac
      661 tttctctcta cccgatcccc gcctattcta aaggcctacc ttatgggcct acctcgttct
      721 ggagggagag ttccccgacc gagatgctca ccgcgtcccc ggcccccaac gagaaatcgt
      781 cctccgggac tatgcccgtc cccgtcatca ggaaggcccc ggacgggaag gcgagctccc
      841 taccgagata ctccgcgagt tcctccgggg accgcttcat ctgcgaggta tcgacctcgc
      901 cgcggaagac gctctcccca ccgcgccgta tctccaactc tatgcgaagc tccaacatcg
      961 cctcggggcc acagagaacg atgccgggcc cgagcgagca cgaaccatca tagaccttgg
     1021 cctgcgggag atagagcggg ttctccccct cgatgtcccg cgaggagacg tcgttgccgg
     1081 cggtatagcc gaccacctcc atcccgctgt tgacgaccac gacgagctcg ggttcgggca
     1141 cgttccagtg gctgtcctcg cgcacgcgta ccggatggcc gtgccccacg acccgccagc
     1201 cgggggcctt gaagaacaac tccggcctct cggcgacgta gaccctctcg taagcgtcgg
     1261 cgtcctcgga ctccatctcc cgcgcgtccc tgctctgcag gtacgtcacc ccgctcgccc
     1321 agacctcttg ctcggcctcc acggggggca ggaggggatc atccgccgcc gcttctgggg
     1381 cttgttcctc cagaaaactc cggacttccg ttgccttcat ggcgagtagc cgccgcagcg
     1441 taaactccgg tggcaggtag cgtccgtcta gcgcccagcg cgggcccccg gccgcctcat
     1501 ggcgggagag atacatggtt ttcgagcctc cttcctcgac tggtacaaac taccacgatc
     1561 gggactccgc cgcgcggctc aggaaaggga gaggacctcc ctggccgtct cgagaaaggc
     1621 ccgtagtgcg ggcgacgccc agcgcgctcc gttccacgcc atgagggtgc cgaccaggat
     1681 gggttccttc catcccagct ccgagaggtc gccggccgag agctctgctc ccaccgccac
     1741 ggtgggtagg acggttaccc ccatgccggc gcttacgcag cgctttatgg cctctatgct
     1801 ctgaaactcg agcatctccc tgggcaccac ccctccctca gcgaactgcc tctcgaactg
     1861 gccgcggtag gcgcaaccgg actgcggcgc ctccggcaag agcacggtct ccccgcccag
     1921 atctgggggt gcaacgaggt ctgaggtcgc cagcgcgtgg tccggcggcg ccaccacgct
     1981 tactcgttcc tcccgcagca actccaccga gagaccctcg atgcccacag acggcacgtc
     2041 aaggacgaag gcgacgtcga cccgaccctc ctccagcgcc cggcgcgtcg aacccacgag
     2101 cctgccgacg acggtcggat ggagcgagac cctcacccgg ggatgtgcct cgtggaacgc
     2161 ggtcagcagc ccggggagcc gataggtgag gagcgtctcc ggcgcgctta ctcggagaga
     2221 accagagatc tcacccctcc ccacccccgc gtcggcgacc gcctccccgg cctcccgcgc
     2281 cagacccagc aaccgctctg cgtagtcgag catcaccgcc cccgcctccg tgagcgcgat
     2341 cctccgcccc aacctgtcga agagcggtac cccaagctcc cgctccaggg cggccacctg
     2401 cgtgcttacg ctcgactgca cgtagcccag ctccgccgca gcccgagaga aggaaagctc
     2461 ccgcgctacc gccctgaagg tctcgacctg ccgtaactcc accgttctcc tcctgatatc
     2521 tgttctaaag atagatcaaa tctaaaaact tcgttggaaa cgataatagc acgcgcttag
     2581 gatctgcttg tggtgagacg aagggtgagc ggagggagca ggttcgatgc agaaggacat
     2641 cagggtggag ggagggggtg cgccggggtc caggttgccg gccatggctt tgcttttcgg
     2701 gggggcgctt gggatctcgg ccgccttcgg aatgctgttg ttgctgcccc tgtatgtaca
     2761 ggagctcggg gggaacgagg gagatttcgg ggttatcatg gcctccgcga ccttcacggc
     2821 ggcgctggct atcgggcttg tgatccgcta cccagaggcg ttgcggccga acgtgttgct
     2881 ggcgctcgcc atcctggcgt acgggttggg agcggcgggg gcggcgctgg tcagagacgg
     2941 ctggctgccg ctggtggggg tagggcttgt gctcgggacg gcctgggcgg tggtctacgc
     3001 cgcgtcgccg atggtgatga gcgggatggt caccgaccgg gagcgggcga cctatttcgg
     3061 ttacctgacc ggtacccagc aactaggcat cggcgtcggt ccggtggtcg cgggctctct
     3121 ggccgagggc ggcctcggct tccggggcac cttcctcgtc gccgccgtcc tgtgcgtcct
     3181 ggcggccgtt ctcgtgctgg cggtgggcat cctggctccc gacgcgggtg gatcggtgcc
     3241 ggggtccgac accggaccgg tcgtctcctt ccgggcttcg atgggggaga tcttgcgctc
     3301 ggagggtgcc ttctggctcg tcatgatcct gctcttcgcc tgcctgttta ccacgctcac
     3361 cagcttccag actaccttcg cccgggctca ggacctggac ttctcggtct tctacctctc
     3421 ctacacggcg gcggtgatcc tctcgcggtt cgggatcgcc ggcttcgcag cccgctacga
     3481 tacgcggctc gtgatagcgg ttgccgtcag cctgatggcc ctctcggttg cctccttcct
     3541 gttcgtgggc tcgaacgctg tgctctacgg cctcgcttcc gccgccgtcg gcctcggcta
     3601 cggtctggcc ctcccgtctg cccaggccca ggcggtaaat gtctcggaag agtccgtaag
     3661 gccacgggtg ctgccgctgg ccggtttgct gttcgagacc atgatcctgg ctttccccct
     3721 gctcgcagga tgggtcatct caaacctcgg ctacggagcc gtgttcgtgc tcctggtcgt
     3781 cctgtgcctc ctgcaagccg ggctcgcctg ggaacgctac gtcagggcaa ggcatcaggc
     3841 gtcaggcaac aggcttcagc aagaaaaacc tgagacctga aacctgtagc ctgtagcctt
     3901 ctcctgtagc cttaggtgag actataagga gggtggatga ccacgacgga acggtaccgg
     3961 atcgcttcga tcccgggcga cggcataggt gctgaggtgc tgccggaggg ccgccgggtc
     4021 ctcgaggctg cggcccgggc ctacggcttc gggcttcatt tcgacgagtt cgacttcgcc
     4081 agctgcgatt actacctgga gcacggcacg atgttgccgg cggggtggaa ggagcagctc
     4141 ggcggttacg acgcgttgtt cttcggggcg gtgggctggc ccgagaaggt cccggatcat
     4201 atctccctct ggggttccct ggtacagttc agacgccagt tcgaccagta cgtgaaccgg
     4261 cgtcctgtac gcctcatgcc cggcgttacc tcccccttgc gagaccccgg ggacatcgac
     4321 ttcgtcatcg tcagggagaa caccgagggg gaatactcag aggtcggcgg caggatgttc
     4381 gaaggcaccg aacgcgaggt cgtggtccag gagaccttca tgacccgtac cggcgtcgac
     4441 cgtatcctaa gctacgcctt cgaactggcc tcagaacgag aggccgggca cctgacctcg
     4501 gccaccaagt caaacggcat ctccatctcc atgccctact gggacgagcg cgtgagggag
     4561 atgtccacct cctacccgca ggtctcctgg gaccagttcc acatagacaa cctctgcgcc
     4621 cagctcatcc tgcgtcccga tttcttcgac gtggtcgtgg gatccaacct cttcggcgat
     4681 atcctctccg acctcgggcc cgccctcaca ggcaccatcg gcatcgcccc ttcggccaac
     4741 atcaaccccg agaagcgctt cccctccctc ttcgaacccg tacacggctc agcccccgat
     4801 atcgcgggca ggggcatagc caaccccgtc ggggccatct gggccgcttc catgctgctt
     4861 gagcatgtgg gccagcccga ggcagccgcg gccgtagtcg ccgccatcga acatgccatg
     4921 ggccagaagg agctacgcac ccccgacctc ggtggtacgg ccacaaccga agagtgcgga
     4981 aaggccgtcg ccgataccct ctcaagcaaa tccgtcgagt agggcgcttt gccggtaggg
     5041 gttgaaaagg cttcaggtta caggcaacag gcaacttccc gaaccgccac gcccgagaac
     5101 ccccggatga cagatagacg cgcacttgac gctcctacct aactaagtta agatttcgat
     5161 catcaatcgg aaaggtcgaa gagttgggca tcaaggagcg tagggagagg cagcgtcggg
     5221 aggtgagggg tgggatcctg tctgcggcgc gggagatcgc agcgggcgag ggttggcggg
     5281 cggtgacgat ccggaagatc gccgaacgta tcgagtacag tccgccggtg atctacgagt
     5341 acttcgggtc gaaggacgag atcctgctgg agctggtacg tctgggttac gctgaacaag
     5401 agggaatgtt ggagtcggtg gtagctaagg cgaagagccc agaagaggcc ttgctcggga
     5461 tagcggacgc ctggatggac ttcgccttcg attcaccaga tctctaccag gctatgtacg
     5521 ggctgggggg cgcgcccttc gccgccgaga cccggggtga aggtgagaag atcggagacc
     5581 tggtcggcaa tgttctggag gcgctacagc aggaacacgg caacgagacc ggagacatag
     5641 aaggcaaggt aacactgctg tggagcaccg tccatggcct cgtggcactc accatggtaa
     5701 gccgcatgcc aggaggagag gtgcaagcca gaagactcgc tagacaggcc gcccacgact
     5761 acatcgccgc ctggcaaacc ggataggagg tagacgtgaa cctctttttt tgacgtttac
     5821 ttaacttaga tagatatgtg aacaatattc tggaatggaa gtgatggcat gcggaaagcg
     5881 ttgggcaggg accggaggat agaggagaag ggagaagttc gggcgatgac gaacggtaac
     5941 gggacggcgg gtaccgaggt agtgatgcag cataagggcg ggcctgaggc gctcgaggtg
     6001 gtgcggcgtg gtagcgtgag tgacccgcgg gctggtgagg ccgtcgtgcg ggtggaggct
     6061 gcgggtgtct ctttcgccga ggtacagatg ctcaggggac gctacttcag ccagccgaag
     6121 tttcccttcg tccctggcta cgaccttgtg ggtagggttg
//
LOCUS       DBNGVD010000495         4473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_123711, whole genome shotgun sequence.
ACCESSION   DBNGVD010000495 DBNGVD010000000
VERSION     DBNGVD010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_123711"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="AC16CM_13315"
     CDS             complement(<1..127)
                     /locus_tag="AC16CM_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR3140858.1"
                     /translation="MAGESDRRGQILEAAFEEFSAHGFKGATIKGIARAAELQSPA"
     gene            226..645
                     /locus_tag="AC16CM_13320"
     CDS             226..645
                     /locus_tag="AC16CM_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004267350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 family protein"
                     /protein_id="HIR3140859.1"
                     /translation="MGYPYDGVDLRKHPERYRIGRGEQGVFHAEPYKGELLPLWKFRD
                     EEAARVSSEAIYQKFCEYRADDDFVGMDVARKYLQMGWTRSMRYAKYRAGKKSQPFEE
                     PDPTKLKGASIFKDLYDRVRADERYVELKERHRSRNE"
     gene            693..884
                     /locus_tag="AC16CM_13325"
     CDS             693..884
                     /locus_tag="AC16CM_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140860.1"
                     /translation="MNRRDLLTLESAITAIEEAASAVAREVERDRLRETSLTRLSTVE
                     AELNRSQLALEKIIREETA"
     gene            complement(1197..2360)
                     /locus_tag="AC16CM_13330"
     CDS             complement(1197..2360)
                     /locus_tag="AC16CM_13330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HIR3140861.1"
                     /translation="MDFAGLNALDLFIGFIIVVSVLRGVRTGFLAGVFSLVGVVVGAS
                     VGSRIAPSLLPDGGNPIYGAGITLGSILAFSVLGEVIARSMGGSIRNRLSSPASAKLD
                     GLGGGLLGFALALVLVWAVGVFALQSPTLAGLQPVAQDSRILQTLKERMPSRLLTQAV
                     SELQPLPKIRGPEPEVAAPEGNIVNDPDVRAASQRTLRVTGTACGYGVEGSAWVGGRN
                     LVVTNAHVVAGETSTWVQVRGIGRQLPAEVVAFDKRNDVSILRVAGLGLPPLPVAPAR
                     PGEEAAVIGFPQNGPLDIEPARTGTTERVFSTDAYDNGPVERIVTSFRVYVRPGNSGG
                     PAVNADGEVISTIFASRTDSNNSGYGIPSRIIQRHLQTAEDRTAPVSTGGCAN"
     gene            2704..3744
                     /gene="rlmN"
                     /locus_tag="AC16CM_13335"
     CDS             2704..3744
                     /gene="rlmN"
                     /locus_tag="AC16CM_13335"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3140862.1"
                     /translation="MDVKKFLPEVQKVLERDGQPSYRLGQAYSALTGSLVRDWEEATS
                     LPQGLRGTLNEEAPAAVLDLRRTWRASDGTRKYLFYTHDRHAIETVMIPEKGRHTVCI
                     STQVGCPMACKFCATGLLGIKRNLKTREIAEQVFVAARDIAPERVSNVVVMGMGEPML
                     NYRATLEALKVLNDPKGFGLAARHIAVSTSGLVDRIRRFADEPEQFHLAISLHTPFED
                     ERSEIMPVAARHTIPELMEAARYYVTSTRRKLFFEYTLLAGVNDQPRHVEALARLLDH
                     PLYHLNLLRFNWTDTGFSATSKRDAREFLRYARELGLSATLRPSRGQDIDAACGQLAA
                     KDLRGRPAVPTI"
     gene            3745..4227
                     /locus_tag="AC16CM_13340"
     CDS             3745..4227
                     /locus_tag="AC16CM_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3140863.1"
                     /translation="MLKGEPAERLDPRAIKLWRLTGLINLLPLLVGAVVAGWALRRFG
                     DYSLFVAVLPALVILVLGSMSVGVAPGLRWRMWRYEIRTDEIDLQRGILWIERTLVPL
                     ARIQHVDTRQGPLQRRFGLSTVVFHTAAGPNQIPELSTPVAAEVRDRIAELTGEQDEL
                     "
     gene            4217..>4473
                     /locus_tag="AC16CM_13345"
     CDS             4217..>4473
                     /locus_tag="AC16CM_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140864.1"
                     /translation="MSSERHLHPVAMIIGAVSTLRRWIGGLVFPGIVLLMSQGFSLRT
                     VSLFLLAVIVVVTLAAVWGFLSWRATTYLVTANSFRLKRGV"
BASE COUNT          838 a         1449 c         1446 g          740 t
ORIGIN      
        1 gcgccggaga ctgcaactca gccgcccgcg caatgccctt gatcgtggcc cccttgaacc
       61 catgcgcgga aaactcctcg aaggccgctt ccagtatctg cccccgcctg tccgactccc
      121 cagccaacgc agccccttta ttgatcgttt aatcaacaac tatagacttg atagaacgtt
      181 cagtcaagag gtaataccga cacgaaatat gggtaaaggg agcatatggg ttatccgtat
      241 gatggtgtgg atctgagaaa gcatcccgag aggtacagga tcgggcgagg ggagcagggc
      301 gtcttccacg ccgagccgta caagggagag ttgctcccgc tgtggaagtt cagggacgaa
      361 gaggcggccc gcgtgtcttc cgaggccatc taccagaagt tttgcgagta tcgcgcggac
      421 gacgatttcg ttgggatgga cgttgccagg aaatacctgc agatgggatg gacgaggagc
      481 atgcggtacg ccaaatacag ggccggcaag aagagccagc cgtttgagga gccggatccg
      541 acgaagttga aaggcgcctc catcttcaaa gacctctacg accgggtgag agccgacgag
      601 agatacgtcg agctcaaaga aaggcacagg agccgtaacg agtagcggcc ccggcaacaa
      661 cgagcgtcac gccttggggt aatatccacc ccatgaaccg acgagaccta cttaccctgg
      721 aaagcgcgat caccgccata gaagaggccg cctccgccgt ggcccgcgag gtcgagcgtg
      781 accgcctgcg cgaaacctct ctcacccgcc tctccacggt agaggccgag ctaaacagaa
      841 gccagctagc actcgagaag ataatccggg aagagacagc ctgaaagcac gccgcttcgc
      901 ggcttgtcag cacgctcggg ctcacgccct cgctcgtcag ctcgcttcgc ccggctgcgc
      961 ctagagttcg ctgtcagcca gatccctctg cggcctgcac ccttccgccc tgaagcttcg
     1021 atgcctggga aaggtgggta gtcgaagcca ccgttacgaa gagagacgac tagctgaaag
     1081 cgaggcgaag ccgaagcgtt gctgacaagc gacagccgcc aggctggagc gtgctgacaa
     1141 acggaggccc gcagggccgt agtgtgctga caagcgtagg cgttagccgg agcgcgctag
     1201 ttcgcgcatc ctcccgtgct gacgggggcc gtacggtcct ccgccgtctg caggtggcgt
     1261 tggattatgc gggaggggat accgtagcca gagttgttcg agtcggtacg gctggcgaag
     1321 atggtcgaga tgacctctcc gtcggcgttt acggctgggc cgccggagtt gcccggcctc
     1381 acgtagacgc ggaagctggt gacgatgcgc tccaccggac cgttgtcgta ggcgtcggtc
     1441 gagaacacgc gttcggtcgt ccccgtgcgg gccggttcga tgtcgagcgg gccgttctgg
     1501 gggaacccga tcacggccgc ctcctccccg gggcgggccg gcgcgacggg aagtgggggg
     1561 aggccgaggc cggctacgcg caagatggag acgtcgtttc tcttgtcgaa ggcgaccacc
     1621 tcggcgggca gttgccgtcc gatgcccctg acctgtaccc aggtcgacgt ttcaccggcg
     1681 accacgtgcg cgttggtaac gacgaggtta cggcccccca cccaggccga gccctcgacc
     1741 ccgtagccgc aggccgtgcc cgtcacccgc agcgtgcgct ggctcgccgc ccgcacgtcc
     1801 gggtcgttga caatgttgcc ttcgggggcc gccacctccg gttcggggcc cctgatcttc
     1861 ggcagcggtt gtagttcgga taccgcctgc gtgaggagtc gggaaggcat cctctctttc
     1921 agggtctgaa gaatcctgga gtcctgtgcc acgggttgca ggccggccag ggtcggggac
     1981 tggagggcga acacgccgac cgcccagacg agcacgaggg cgagggcgaa ccccagcagg
     2041 cccccgccaa gaccgtccag tttcgcagaa gccgggctgg agaggcggtt cctgatcgac
     2101 ccccccatcg acctcgcgat gacctccccg agcaccgaga aggcgaggat gctgccgagc
     2161 gttatcccgg caccgtatat agggttcccg ccatcaggca ggagagacgg cgcgatcctc
     2221 gaccctacag acgcaccaac gaccacgccg accagcgaga agacgccggc cagaaacccc
     2281 gtccgcaccc cgcgcaagac cgatacgaca atgataaagc cgatgaaaag gtccagagcg
     2341 ttgaggccgg caaagtccat ttatagattc tacatcagca cgctccggct aacgcctggg
     2401 cttgtcagca cgctccagcc tggcggctgt cgcttgtcag caacgcttcg cccggctccg
     2461 ccagggctcg ctttcggcca gatcccgctg cggcctgcac cctgccgctc tgaagattcg
     2521 atgctcagaa caggtggtta gtcgaagccg ccgtaacgga tagagacgac tggccgaaag
     2581 cgaggcgaag ccgatgcgtt gccgacaagc gagggcaaaa gcccgagcgt gctgacaagc
     2641 ggaggcgaag ccgaagcgtg cgcaagtcgg cgcagccggc ttgcacggta taatcgctcg
     2701 actatggacg tgaagaagtt tttgccagaa gttcagaagg tgctggaacg ggacggccag
     2761 ccgtcctacc ggctcggcca ggcctactct gccctcaccg gctcgctggt cagggattgg
     2821 gaggaggcta cgagcctgcc gcagggcctg cgcgggacgc tcaacgagga agccccggcg
     2881 gcggtcctgg atctcaggcg cacctggagg gcttccgacg ggaccaggaa gtacctgttc
     2941 tacacccacg atcggcacgc tatagagacc gtcatgatcc ccgagaaggg gcgtcatacc
     3001 gtgtgcatct ccacccaggt ggggtgcccg atggcctgca aattttgcgc cacggggctg
     3061 ctcggcatca agcgcaacct gaagacgcgc gagatcgccg agcaggtctt cgtcgccgcc
     3121 agggatatcg ccccggagcg ggtttcgaac gtggttgtga tggggatggg cgagccgatg
     3181 ctcaattaca gagccacgct cgaggcgcta aaggtcctga acgaccccaa aggcttcggg
     3241 ctcgcggcaa ggcacatagc cgtctccacg agcgggctgg tggacaggat ccggcgtttc
     3301 gccgacgagc ccgagcagtt ccacctggcc atctccctgc acactccttt cgaggacgag
     3361 cggagcgaga tcatgcccgt agcagcccgc cacaccatcc ccgagctcat ggaagcggcc
     3421 cgctactacg ttacgagcac gcgtcgcaag ctcttcttcg agtacacgct cctcgccggc
     3481 gtcaacgacc agccgcggca cgtcgaggcc ctggccaggc tcctcgacca cccgctctac
     3541 cacctcaacc tgctccgctt caactggacc gacacgggct tctcagccac ctcgaagcgc
     3601 gacgccaggg agttcctgag gtacgccagg gagctcgggc tctcggccac gctgcgcccg
     3661 agccgggggc aggatatcga cgccgcctgc ggccagctcg ccgcgaagga cctgcggggg
     3721 cgccccgccg ttccgaccat ctaggtgttg aagggcgagc ccgcagagag gctggaccca
     3781 cgggcgatca agctgtggag gcttacgggg cttatcaacc tgcttcccct cctcgtcggg
     3841 gccgttgtcg ccggctgggc gctccggagg ttcggcgact actcgctttt tgtggccgtc
     3901 ctgcccgcgc tcgttatcct cgtgcttggc tcgatgtccg tcggggttgc accggggctg
     3961 cggtggcgga tgtggcggta tgagatcagg acggacgaga tcgacctcca gcggggcatc
     4021 ctgtggatcg aacgcacgct cgtgccgctg gcccgcatcc agcacgtgga caccaggcag
     4081 ggacctttgc agcgccggtt cgggctctcc acggtcgtct tccacaccgc ggctggtccg
     4141 aaccagatcc ccgagctctc caccccggtg gccgccgagg taagggaccg catcgccgag
     4201 cttacggggg agcaggatga gctctgagcg gcacctccat cccgtagcaa tgatcatcgg
     4261 ggccgtgagc acgctgcggc gttggatcgg cggcctggtc ttccccggca tagtcctcct
     4321 gatgagccag gggttcagcc tgcgcacggt ctccctcttc ctgctagccg tgatcgtcgt
     4381 cgtcaccctg gcggccgtct ggggcttcct ctcctggcgg gcaaccacct accttgtgac
     4441 ggccaactcg ttccggctca agcggggggt ctt
//
LOCUS       DBNGVD010000496         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2856348, whole genome shotgun sequence.
ACCESSION   DBNGVD010000496 DBNGVD010000000
VERSION     DBNGVD010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2856348"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16CM_13350"
     CDS             <1..493
                     /locus_tag="AC16CM_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR3140865.1"
                     /translation="TWSKPNPPPNLLHTAFTHSHETLLWASRSKYSRHTFNYDLINGP
                     DPTSQLSSVWHIPAVPMREKVHGRHPTQKPLRLLRRAVLASTREDDLMFDPFSGSGTT
                     GVAAKELGRFFVGAETEREYVGLAERRIGAVERGTVLEKIRSTGAEDLLPGYRARRRA
                     SPD"
     gene            1057..1881
                     /locus_tag="AC16CM_13355"
     CDS             1057..1881
                     /locus_tag="AC16CM_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140866.1"
                     /translation="MTNSRNDRAQGAGDYILVNGLKMYYEIHGAGQPLVLLHGAFSAI
                     GTSFGNVLPELAKSRRVVAFEMQAHGRTADIDRPLSMEQMADDTVAALEQLGIENADF
                     FGFSMGAGIALRIAIRRPEVVRKLVLASVTYNESGLHPGLMDGLAEMKPEMMHGSPWH
                     EEYVRTAPRPEDFARLFAKKMQMDREIQDLHAEAIEAIGAPTLLVIGDSDIVRPEHAV
                     EMFRLLGGGVMGDLAGLPRSQLAVLPGTTHATLVEHGEWLGSMITKFLDLPMPDAE"
     gene            complement(1978..2199)
                     /locus_tag="AC16CM_13360"
                     /pseudo
     CDS             complement(1978..2199)
                     /locus_tag="AC16CM_13360"
                     /inference="COORDINATES: protein motif:HMM:NF018213.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
     gene            complement(2218..>2483)
                     /locus_tag="AC16CM_13365"
     CDS             complement(2218..>2483)
                     /locus_tag="AC16CM_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3140867.1"
                     /translation="RELFGEGVSSLQTTRRTYNFRYPSADHYVGWFREYYGPTVRAFA
                     ALDAEGQEALASDLRELCESRNVSGDGTLVAPSDYLEAVAIRR"
BASE COUNT          487 a          789 c          787 g          420 t
ORIGIN      
        1 cacctggtcc aagcccaacc cgccaccgaa ccttctccac acagccttca cccactcaca
       61 tgagacattg ctgtgggcga gcaggagcaa gtactccagg cacaccttca actacgacct
      121 cataaacggc cccgacccaa cctcccagct ctcctccgta tggcacatcc ccgccgtgcc
      181 catgagggag aaggtccacg ggcgtcatcc cacccagaag cccctgcgcc tcctcaggcg
      241 cgccgtgctg gcctccacca gggaagacga cctcatgttt gaccctttca gcgggtcagg
      301 aacgacgggg gtagcggcca aggagttggg ccgcttcttc gtgggggccg agacggagcg
      361 ggagtacgtc gggttggcgg agcgtaggat tggtgcggtg gagaggggta ccgtgctgga
      421 gaagatccgg agcaccggcg ccgaggacct tctgccaggg taccgagcgc gacggcgggc
      481 ctcgcccgac tagcgccatg ccggtatcac gatttctact aacacctaga tcctgaccac
      541 agcgaggagc ggcgcaacgc ctcacacgcg gcaacgctcg gcaactccaa ggttggggca
      601 aagatacgga gcacgcggct gatagtcagg gagcctttgg aggtaacgct ctccggcgac
      661 ctccaccccg taatcaggaa gcgtctagcc gaggtcgtcc agttagtcca agcctaatgc
      721 cgcctcgcgg agcttgagat cgccgccggg gagaggccga aggccggtga ctcgaagttg
      781 ccagaagatt tgggagagcg cgtgagggag tgggccgagg gcgaggcaga gagaaaagca
      841 gagggcggcg ttcatgggga gatcacaggc tatcggcgag gatccgaggg cggtgctgaa
      901 ggagatgggc ggctagggct cgtggggctc caaggatgtc tgctcctttc tccctcggga
      961 gctcctgttc catattgtag atatcagttg tattcagtgg ccgttcgctt atgctgcgac
     1021 cagacgctgc caaccaccga ggaaggagca cccaccgtga cgaacagccg caacgaccgg
     1081 gcccaagggg cgggcgacta catcctcgtg aacggcctca agatgtatta cgagattcac
     1141 ggtgccggac agcctctcgt cttactccac ggcgcttttt cggcgatcgg cacctcgttc
     1201 gggaacgtgt tgccggaact cgccaagtcc aggcgggtgg tcgccttcga gatgcaggcc
     1261 cacggacgca ccgccgacat cgatcgcccg ctgtccatgg agcagatggc cgacgacacc
     1321 gttgccgctc tcgaacagct tgggatcgaa aacgccgact tcttcggttt cagcatggga
     1381 gcaggtatcg ccctgcggat cgccattcga cgcccggagg tggtgcgcaa actcgtgctc
     1441 gcctcggtca cctacaacga gagcggcctc caccccgggc tgatggacgg tttggcggag
     1501 atgaaacccg agatgatgca tggctcgccc tggcacgagg agtacgtgcg tacagcgccc
     1561 aggccggagg acttcgccag gttgttcgcc aaaaagatgc aaatggatcg agagatccaa
     1621 gacttgcacg ccgaggcgat agaggcgata ggcgccccta ccctgctcgt catcggcgat
     1681 tccgatatcg tccgccccga gcacgccgtg gagatgttcc gtttgctcgg cggcggggtt
     1741 atgggagacc tggcgggcct accgcgttcg cagctcgcag tccttcccgg caccactcat
     1801 gcaacgctcg tcgagcacgg cgaatggctg ggttcgatga tcacgaagtt cctcgatttg
     1861 ccaatgcccg acgccgagtg agtcgcgatg ttaggaaatc ctttttgggc tgagggcaga
     1921 ccgttacgtc tgtggaggag acccaagcaa gcagggggat tagttcaccc acttgctcta
     1981 cccggaccag gcccgtcgga aaagcccagg gtagtcgatc accaggccgt cggcgtcgac
     2041 cggaagcgca gctgtgaacc cgcttggcag cgcctcgaac tggtaaagcc cgtcgtttgc
     2101 ccctgcctcc aggcaaccgt accgctgccg ctccgctcct accagcagct cgggaacgcg
     2161 gacgtaggcg acccttagct cttcggattg gccgggcccg aaccccgagc gatgggatca
     2221 acgcctgatg gccaccgcct ccaggtagtc cgatggcgcg acgagcgtcc cgtcgcccga
     2281 gacgttacgg gactcgcaca actccctgag atcggaggcc agggcttcct gcccctccgc
     2341 gtcgagcgcc gcgaacgccc tcaccgtcgg gccgtagtac tcgcggaacc accccacgta
     2401 gtgatcggcc gacgggtacc ggaagttgta ggtcctgcgc gtcgtctgca aggaggagac
     2461 gccctctcca aagagttctc gca
//
LOCUS       DBNGVD010000497        14238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3403243, whole genome shotgun sequence.
ACCESSION   DBNGVD010000497 DBNGVD010000000
VERSION     DBNGVD010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3403243"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC16CM_13370"
     CDS             <1..483
                     /locus_tag="AC16CM_13370"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3140868.1"
                     /translation="GTPREVYEEPASAYVADFLGVSNLLDAEAVGETSDGRCRLRAGD
                     LELLASHGHTSARGQVKVVVRPERVRVEAPGRTGENRLPGRIERVVYAGPISQLVVTL
                     DGGEHIQCMLANDGVDESFERGAPVSVYLPCEALRVLGTAGNDEENESFATTARAVAD
                     "
     gene            731..1288
                     /locus_tag="AC16CM_13375"
     CDS             731..1288
                     /locus_tag="AC16CM_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140869.1"
                     /translation="MIAQINPETQAMVDFGRARRRALIRRLGNAMRKDDFSLPAFEEV
                     RRSLGVRNRLPIARTEAVEVEKIIGSVGRSNDFDACFLPVRPDVSSRWESVDSAFQRG
                     KELPPVSLYKMGDAYFVNDGNHRVSVARYHGIESIDAEVVTFRSPRVTPAEILGALPN
                     CSGWKSRMRDKLKSALSSRKRTRNR"
     gene            complement(1726..2643)
                     /locus_tag="AC16CM_13380"
     CDS             complement(1726..2643)
                     /locus_tag="AC16CM_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140870.1"
                     /translation="MSAPSDAGRNTALTRLRMWWSNPWGRPRFLIIFTWAYVLWSLAP
                     VAVAVLFGFNDGRSRSTWQGFSLRWYFQDPDLSVLHDPSLRDALFQSLKLAGLTMLIA
                     TPLGVALAIGLARWRGRGARPANFLMLFPLVTPEIVMGVSLFLVFVYLFGFIQLGTQA
                     QLLGHVTFTISYVVIVVRGRLFAVGREYEEAAMDLGASQWQAIRMVLLPMLAPAIWAS
                     LMIAFAISIDDFVITAFLMGDQTSATIPVKLYSAARAAPSPALNALASILLFASMLAI
                     SVALLVMRRSQKKEGAKRSAVEDFARLEI"
     gene            complement(2640..3560)
                     /locus_tag="AC16CM_13385"
     CDS             complement(2640..3560)
                     /locus_tag="AC16CM_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3140871.1"
                     /translation="MSKPADERGAVGEGRWLWPGFALPGVVWLIILFVTPLYAIMAMA
                     MGTSDPVFGDTLPVWNPLQWNFATFGDVLGELFTGQLGVVFLRTLVYVFVAAGLSLLI
                     GYPVAYYIARHAGRYRVLLLLLIIAPFWINYLMRMLAWVNLLSTDGYVNRILMFVGIL
                     DAPREWLVGKPETVVLGLVYGYVPFLILPLFAALDRIDQRVIEAARDLGASPAQAFFR
                     VTLPLSYQGMLAGCVIIMLPMFGDYYTATLLSGSPKTNMIAKLIEQNINSSAGGNTGA
                     ALTLVLMVFVGLLMTYYLFSVARATREARS"
     gene            complement(3565..4827)
                     /locus_tag="AC16CM_13390"
     CDS             complement(3565..4827)
                     /locus_tag="AC16CM_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3140872.1"
                     /translation="MGFHPKQRPFWPPSMSRREALRLMAGAAMAGSVLAGCGSGADNS
                     SAVKIVAGTRDRPVKQPLYEDNPMIESGLEPEKGPLRIYNWADYMYTRVLKDFEKEFG
                     VEVELTTFYNLEEATRKLRTGKVDFDVFVPTAEIIPKFVAGKLMQPLNHSYLPNLKKN
                     VWPMLADPYYDQGSRYTVPYTVYLTGIGWRTDMVSADIAGMDNPWEAFWNPEYKGIAG
                     LYDEYREAIGVGMYRSGITDINGDKKSDLNKATDNLIELTDLVDIRYTIDGAYAKLPE
                     SAFGIHQAWSGDMVNAPYYMPKGDDPSVLRFVWPPKAQGGAGGYVSNDSLAVLNGAEH
                     PVLAHMFLNYMLDDKVAVKNFSWNAYQPPINALKPDKLVSDGMIRENLASTVIEQGDF
                     DMGQIPEQLTPEEDKHWLEAWSRVQQGG"
     gene            complement(4838..5458)
                     /locus_tag="AC16CM_13395"
     CDS             complement(4838..5458)
                     /locus_tag="AC16CM_13395"
                     /inference="COORDINATES: protein motif:HMM:NF025343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3140873.1"
                     /translation="MLEAAAEVIGERGLCDTRIADVAGRAGLSPALVVYYFGSKEKLL
                     TEALAYAEDLFYIEAFRELTGIEGAGDRLARLIELACPPVERRETGEYWALWVELWSR
                     SLRNGEAARKREALDRRWRNTIAEVVREGQRRGEFGPCDPERFATYLSALMDGLALQM
                     LLKDSAVDGAMVNGMCLHAAARELDLDPAALERGNGPSGGEYATEG"
     gene            5688..7322
                     /locus_tag="AC16CM_13400"
     CDS             5688..7322
                     /locus_tag="AC16CM_13400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3140874.1"
                     /translation="MKGDFDAIVIGGGHNGLAAGAYLARDGLRTVVLEARHKVGGAAS
                     TDAPWPEAPELNVTTYSYVMSLMPRRVIDELRLERHGYKVYPMGPSFYAFPDGRSLTI
                     TGTDDKRDFEQMARFSRRDAEAYGEWRGWLAEQAAIMEPLLLTTPPKVGSRHPLDLLE
                     QLKLAWKMRGLDVARVGELTRLMTMSAWDLLDRWFESPEIKGAMAVDGIIGTWAGPAS
                     PGTAYVLMHHEIGDAGLGLSSWGYPRGGMGAVSDALRSSAEEFGAEVRTNAPVERILQ
                     KNGRVTGVVLEGGDELEADIVVAATHPRTTFLEQLRPDELPGEFVDSIERWNSRSGVV
                     KINLALSELPDFTSNPGKGDHLGGAIELAHSIDYIEEAFQDARRGEPARRPFSDGVIP
                     TVFDRTLCPEGYHIMSLFTQWVPHEWSEEPHRAELEAYADRVVDGYTELAPNLKDAIL
                     HRQVIGPYDMEQEIGLFGGNIFHGELSAEQLFHMRPAPGYADYRTPIRGLYQASSATH
                     AGGGVCAIPAYNCVREIRRDMRRERIKERLTPRSRG"
     gene            7327..9432
                     /locus_tag="AC16CM_13405"
     CDS             7327..9432
                     /locus_tag="AC16CM_13405"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3140875.1"
                     /translation="MALDGATRAMLGARSVAVVGASPKFDSPGNHMVRQLAVGGFSGR
                     VAAVNPRYDEVEGFSCFPSLGALPFAPDLVLLGVGNHRLEEQMEAAASCGARGVVVFA
                     SALEAPPRDPPLAERLRRIAEGAGMAVCGANCMGFLDVERGLRALAFEERTDLKPGNV
                     AWISHSGSAFSALLHAERGIHFNLAVSTGQELRTTMADYMLYALERDSTRVISLFLET
                     IRDPDRFRLALATAAERDVPIVALKVGRDPASRRLVAAHSGALAGDDAAYEALFDAYD
                     VARVGTLNEMADLVELLAAGRRAARGGLAAILDSGGERAHLVDSAAGLRVPLAQPSEK
                     TLKSLAARLEPGLPSVNPLDAWGTDDGAYATFLDCSRHLAADPDTGAFAYVVDLHSER
                     AERGHAWAAERVWSATDKPVAVVCGLTSAIQDSAADQLRSGGIPVLEDVNSGLLAFRH
                     LFDRRDSRALASTPPPDAVPDHIRLRWRETLASGPRPGEDEALEMLADYGIPVAQTVA
                     VDSLDGALASAQRLGYPVALKTAAASHKTDVGGVRLGIEYPAALTAAYGEMSSRLGPE
                     VVVQEMVGPGVEMALGIVHDPQFGPLVMVAAGGILIETLADRRLALPPLNEAHARRLV
                     DRLGAVRLLDGVRGGPPADRYSLAHALSRLSVLAHDLGDLIDALDVNPLIVGEKGCTA
                     VDALVEPSTTGTRELTADG"
     gene            complement(9437..10624)
                     /locus_tag="AC16CM_13410"
     CDS             complement(9437..10624)
                     /locus_tag="AC16CM_13410"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3140876.1"
                     /translation="MTPLDRELLVSSVMPDGQMSLPRAAYTEDSVLAWEMEHFFDESW
                     VCVGRSEDLRSPGDRKAVALGHERALLVRGEDGVLRAFYNVCRHRGHDLLEVGCSAGG
                     KFIRCPYHAWAYGLDGRLQGTPGFGGSFGFDKEDYSLLPVRLAEWHGWVFLNASGDAP
                     SFEDHVGDLGDRISHHGPGELVTAAGQSYEVAANWKILAENYHECYHCPSIHPELCKV
                     SPPKSGEDYESTGAWVGGPMELEDFAETMSLDGRSHAPRLPHLAEGQEREVYYFQLFP
                     NLLVSLHPDYIMTHRLRPLTPDRTAVECEWLFSREATEQEGFNPSYASDFWHITNGQD
                     WAACEGVQRGASSRGYRQGPLSPEESTVGKFVGMVARGYLEGGVVQIPDWTAPERTPS
                     QYR"
     gene            10938..12116
                     /locus_tag="AC16CM_13415"
     CDS             10938..12116
                     /locus_tag="AC16CM_13415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3140877.1"
                     /translation="MPVYDVVVVGGGNLGLWTAYRLARRGFGRVAVLERGWAGGGATS
                     RSAGVVRQQGGSETAARLGKLSRQLYLSLGEELGLDSGFRETGYYIVAETEEEKEGFM
                     RLIGVRREAGVENEWVTPEEGRRRFPDLAWDRFVGATYTAGDGYVHPPIAARNATCAV
                     LSEEAVELFEMCEVLGIEERGDHYAVETAGGLFEAERVVDAGGPRGAHAVGAMVGVEV
                     PVMAVRHQIVTFPVLGGDAIHPFPLVFNVGKGYYWRPEEQGALLGMSNPVDEADTSGR
                     YQIGFDWDYYEKMRPDWESAHPALAGLPVSRAWAASIDYTPDHLPIVDQPREGFYVLA
                     AGGHGMMWGPALGEAMARLISEDTLGDLPKEDVELSRFSREKDPNRLQDAIALPFPKR
                     "
     gene            12130..13314
                     /locus_tag="AC16CM_13420"
     CDS             12130..13314
                     /locus_tag="AC16CM_13420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3140878.1"
                     /translation="MTTVPEQQILPEIPEEVRELKDRAARFVTEKLHPFEVEIAERGE
                     IDEDRLRALRDEARAAGLSNLNMPTEYGGMDLSMASLVALEEESGKTTNGLGYMVADR
                     GPRELLELASPEQVERYVLPVVRGETREAWAITEPEAGSDIQDIKTTAQRDGEGWVLN
                     GEKWFVTGGERAGFFIVLAWEGGEQASFLVDRDTPGLEIVRTPTFMHDPYASKHVELR
                     LDDCLVPAGAKLGSGGDEGTKRWFSLERLMIAARCCGAAERLVDLARSWSLERSASGR
                     KLAEYQAIQFMLADSLTELNAARLMTYHVARSWEREDARILHGKTAMAKLFASEMAGR
                     VADRAVQTFGGRGYMTENPVERYYRELRVDRIWEGTSEIQRTIVARGMLKRGAEAYMM
                     NE"
     gene            13339..>14238
                     /locus_tag="AC16CM_13425"
     CDS             13339..>14238
                     /locus_tag="AC16CM_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3140879.1"
                     /translation="MAREKIFIDGEFAEGADGEWRKVLNPATREVIAEVPECSGEDVD
                     RAVGAARKAFGGWFDSTPAERSRMLHDLADVLEENAEELAQLESRNVGKPISSAREEL
                     PYIVDNLRFFAGMARDVEGKSAGEYTRGYTSMIRREPVGVVGQIAPWNYPLMMAVWKI
                     GPALAAGNTVVLKPSEQTPLTTLRMAELAADIFPRGVLNVVTGDGEPVGAGIVRHPGV
                     DMVSLTGDVATGKAVARAASDTLKRVHLELGGKAPVVVFDDADLEEVVAGIRYAGYAN
                     SGQDCTAAARVIAGPGIYEEVSSR"
BASE COUNT         2546 a         4483 c         4799 g         2410 t
ORIGIN      
        1 ggcactcccc gcgaggtcta cgaagagccc gcgtcggcct acgtcgcgga ctttctcggc
       61 gtctcgaacc tgctcgacgc cgaggccgtc ggcgagacgt cagacggcag atgcaggcta
      121 agggccgggg atctggagtt gctcgcctcc cacggccaca cctctgccag gggacaggtc
      181 aaggtggtgg tgcgccccga gcgggtgcga gtggaggccc caggaagaac cggggagaac
      241 cggttgcccg gcaggataga acgcgtcgtg tatgcaggcc ccatctccca gctcgtcgtc
      301 accctcgacg ggggagagca tatccaatgc atgctcgcca acgatggggt agatgaatcc
      361 ttcgagaggg gtgcaccggt ctcggtgtac ctgccgtgcg aggctcttcg ggtactaggc
      421 accgccggga acgatgaaga gaacgagtcc ttcgccacaa ccgctcgggc tgtggcagat
      481 tagagcggtt cgcggaaaga gcgaactcga ggtgagtatg ggcaacaggc gtcaggtaat
      541 ttgtcctgaa acctgtggcc tgatgccttc ctgcccagcg cctcgactcg ctcccgtatc
      601 gctataagtc tcacgttctc tctccgccaa aggtctggtc gcaaatggtg gccttcggcc
      661 gatgccccga tcggcattcc tgacgatact gtcagcacag atcacgagta gagtggaggt
      721 cgaataactc atgatcgctc agataaaccc cgagacacag gcgatggtcg acttcggtcg
      781 cgcccgcagg agagctttga ttcggcgcct ggggaacgcc atgcgcaaag atgacttcag
      841 cctgccggcc ttcgaggagg tcaggaggtc actgggggtt cgcaatcgac tcccaatcgc
      901 caggacagag gcggtagagg tcgagaagat aatcggcagc gtaggtcgca gcaacgactt
      961 cgacgcctgt ttcctaccgg ttcgcccaga cgtatcgtcg cgctgggaga gcgtcgacag
     1021 cgctttccag cggggcaagg agctgccgcc ggtgagcctg tacaagatgg gggacgccta
     1081 cttcgtcaac gacggtaacc acagggtctc cgtggcccgc tatcacggca tcgagtccat
     1141 agacgccgag gtggtaacgt ttcgctcgcc ccgagttact cctgccgaga ttctgggtgc
     1201 cttgccgaac tgctccggat ggaaatcgcg tatgagagac aaactcaaat ccgctctctc
     1261 ctcgcgcaag cgtacacgga accggtaagt agacctacgg cgatgaccac gacaagtcaa
     1321 tcggcgtccg gtagccgcaa tgaggccgcg aggatagctg acgccgcacc aagacgccgg
     1381 gcaaacgtca cttgaaggac tacgggtacc agagatacga gaagttacgt atagcgtgcc
     1441 accatcattg tggttaatct gaggacaaga tgcaattgat acaacctccg ggggcgcgga
     1501 catcctgtcc tcctccggcc gtataaggac acggcagccg cattgaaagc ggaggcgcat
     1561 cggaggttgg aggcggggac cttcttcggg cacatcgcct atgctagcct caccgcccgc
     1621 aagcccgcgt aggcgccaac caacgctgac gtttccgtgg gcagagctac ctgttgctcg
     1681 accccctacc gtgatcagaa cggttctcga acggcctaga tgagttcata tctccaaccg
     1741 cgcgaaatcc tcgacggcgg accgcttcgc gccctccttt ttctgggaac ggcgcattac
     1801 cagcagggcg acgcttatgg cgagcatgga ggcgaagagg aggatgctgg ctagggcgtt
     1861 cagggccggg ctgggggcgg cacgcgcggc gctatagagc ttgaccggga tggtggccga
     1921 ggtctggtcg cccatcagga aagccgttat cacgaagtcg tcgatggaga tggcgaaggc
     1981 tatcatgagg ctcgcccaga tggccggcgc aagcatgggc aacagaacca tccttatggc
     2041 ctgccactgc gaggccccga ggtccattgc cgcctcctcg tactcccgtc cgacagcgaa
     2101 gagccgaccg cgcaccacga tcaccacgta tgagatggtg aacgtgacgt gcccgagcaa
     2161 ctgcgcctgg gtgccgagct gtatgaagcc gaagaggtac acgaacacca ggaacagcga
     2221 gacgcccatc actatctccg gcgtgaccag cgggaagagc atcaggaagt tggccggccg
     2281 cgctcccctt ccgcgccagc gagccagccc gatggcgagc gcgaccccca gaggcgtggc
     2341 gatcagcatg gtcaggccgg cgagcttgag gctctggaag agggcgtccc tgagggaggg
     2401 gtcgtgcaac acggagaggt cgggatcctg gaagtaccac cgcagcgaga aaccctgcca
     2461 ggtgctgcgg gagcggccgt cgttgaaccc gaagagcacg gccacggcca cgggggccag
     2521 cgaccacagc acgtaggccc aggtaaagat tatgaggaag cgtggtcggc cccaggggtt
     2581 cgaccaccac atccgtagac gcgtgagggc cgtgtttcgc ccggcgtcgg aaggcgcgct
     2641 cacgaacggg cctccctggt cgccctggcc acgctgaaca ggtagtaggt catcaggagg
     2701 ccgacgaaca ccatcaggac gagggtcagg gccgcgcccg tgttaccccc ggcgctcgag
     2761 tttatgttct gttctatgag cttggcgatc atgttcgtct tcggcgaccc ggacaggagg
     2821 gtcgccgtgt agtagtcgcc gaacatgggg agcatgatga tcacgcaccc cgccagcatg
     2881 ccctggtagg agagcggcag cgtgacgcgg aagaaggcct gcgcgggact ggccccgagg
     2941 tcgcgcgccg cctcgatgac gcgctggtcg atcctgtcca gggcggcgaa gaggggcagg
     3001 atcaggaacg gcacgtagcc gtagacgaga ccgagcacga cggtctcggg tttacccacg
     3061 agccattccc tgggcgcatc gaggatgccc acgaacatca ggatccggtt cacgtagccg
     3121 tcagtggaga gcaggttcac ccaggcgagc atccgcatga ggtagttgat ccagaacggc
     3181 gcgatgatga gcagcaagag caacaccctg taccgtccgg cgtgcctcgc gatgtagtag
     3241 gccacggggt acccgatcag gaggctcagg cccgccgcca cgaacacgta gacgagcgtg
     3301 cgcaggaaca ccaccccgag ctggcctgtg aacagctcgc caagaacatc cccgaaggtc
     3361 gcgaagttcc actgcaacgg gttccacacg ggcagcgtgt ccccgaagac ggggtccgag
     3421 gtgcccatgg ccatggccat gatggcgtac aggggcgtga cgaagaggat gatgagccac
     3481 accacgccgg gcagtgcgaa gcccggccag agccagcgtc cctccccgac ggccccgcgc
     3541 tcgtcggcag gtttgctcac cgcgttatcc cccctgctgt acccgtgacc aggcctcgag
     3601 ccagtgcttg tcctcctcgg gcgtcaactg ctctggtatc tggcccatgt cgaagtcgcc
     3661 ctgctcgata accgtggacg cgaggttctc gcggatcatc ccgtcggaga cgagcttgtc
     3721 cggcttgagg gcgtttatgg gcggctggta ggcgttccag ctgaagttct ttacggccac
     3781 cttgtcgtct agcatgtagt tgaggaacat gtgggcgagg accggatgct cggccccgtt
     3841 cagaacggcg aggctgtcgt tggagacgta gcctccagcc cccccctgtg ccttcggagg
     3901 ccacacgaag cgcagcaccg aaggatcgtc gcccttgggc atgtagtagg gcgcgttgac
     3961 catgtccccg gaccaggcct ggtggatgcc gaaggcgctc tccggcaact tggcgtaggc
     4021 gccgtcgatt gtgtagcgga tatcgacgag gtccgtcagc tcgatgaggt tgtccgtggc
     4081 cttgttcagg tcactcttct tgtccccgtt gatgtcggtg atgccgctcc tgtacattcc
     4141 gaccccgatg gcctccctgt actcgtcgta gagcccagcg atgcccttgt actcggggtt
     4201 ccagaaggcc tcccacgggt tgtccatgcc ggcgatgtcg gccgatacca tgtccgttcg
     4261 ccagccgatg ccggtcaggt agacggtgta ggggacggtg tagcgcgaac cctgatcgta
     4321 gtacgggtcg gcgagcatgg gccagacgtt cttctttaaa ttcggcaggt agctgtggtt
     4381 gaggggctgc attagcttgc cggccacgaa cttgggtatg atctcggcgg tggggacgaa
     4441 cacgtcgaag tcaaccttgc ctgtcctgag cttcctggtt gcctcctcca ggttgtagaa
     4501 ggtggtgagc tcgacctcga ccccgaactc cttctcgaag tccttgagca cgcgggtgta
     4561 catgtagtcg gcccagttgt agatgcgtaa cggccctttc tccggctcca gccccgactc
     4621 tatcatcggg ttgtcctcat agaggggctg cttgacgggc ctgtctcgcg tgcccgcgac
     4681 aatcttcacg gcggaagagt tatccgcccc ggaaccgcag ccagcgagca cactgccggc
     4741 catcgccgca ccggccatga gcctcagcgc ctcgcggcgg gacatcgacg gaggccagaa
     4801 aggcctctgt ttcggatgga accccatctc taccacctta cccctcggta gcgtactccc
     4861 ctccacttgg cccgtttcca cgttctaaag cggcggggtc gaggtccagc tcccgcgccg
     4921 ccgcgtggag acacatcccg ttgaccatcg cgccgtccac cgcagaatcc ttgagcagca
     4981 tctgcagcgc gaggccgtcc atgagcgcgg agaggtacgt cgcgaaccgc tcgggatcgc
     5041 agggcccgaa ctcaccacgc cgctggccct ccctgacaac ctcggcgatg gtgttccgcc
     5101 agcgcctgtc cagggcctcg cgcttgcggg ccgcctcccc gttgcgcaag gaccgcgacc
     5161 ataactccac ccacaacgcc cagtactcgc ccgtctcccg ccgctctacc ggcggacagg
     5221 ccagctctat gagccgcgcg agccggtccc cagcaccctc tataccggtc aactccctga
     5281 acgcctcgat gtagaagagg tcctcggcgt aggcgagcgc ctccgtcaac aacttctcct
     5341 tggaaccgaa gtaatacacc accagcgccg ggctcaaccc ggcccgcccc gcgacgtcgg
     5401 cgatgcgcgt gtcgcaaagc ccccgctccc cgatgacctc ggcagccgcc tccaacatct
     5461 gtgtgcgccg cttctgtgtc aacctgggcc taccactcat gcggctcccg ccgcctccct
     5521 tccgagttgt gaacggctat ttaatgcgca agatagcata acccgacgcc ctggtccatc
     5581 gaaccggtga actgtccgtc tacgagaaac gtcttgccgc cgcgaccgcg aaatgctaga
     5641 ttgaatgact gtttagtaga ggcttcggct cctgaaggga gagggtgatg aagggcgact
     5701 ttgatgccat agtcatcggc ggggggcaca acgggcttgc ggcgggggct tatctcgcgc
     5761 gtgacgggtt gcgcacggtg gtgcttgagg cccgccacaa ggtgggtggg gcggcgagca
     5821 cggacgcgcc gtggcccgag gcgcccgagt tgaacgtcac cacctactcg tatgtgatga
     5881 gcttgatgcc gcggcgcgtc atcgacgagt tgaggttgga gcgtcacggg tacaaggtct
     5941 accccatggg accttctttc tacgccttcc cggacgggcg atccctgacc atcacgggca
     6001 cggacgacaa gcgagacttc gagcagatgg cgcggttctc gcgccgggat gccgaggcct
     6061 acggggagtg gcgcgggtgg cttgcggagc aggcggcgat catggagccc ctgctcttga
     6121 ccacaccgcc gaaggtgggt tccaggcacc ccctcgacct gctggagcag ctcaagttgg
     6181 cgtggaagat gcgggggctc gacgtggcgc gggtcggcga gctgacccgg ctcatgacca
     6241 tgagcgcctg ggatctcctg gaccgctggt tcgagtcgcc cgagatcaag ggggccatgg
     6301 ccgtcgacgg catcatagga acgtgggccg ggccggcctc ccccggcacg gcgtacgtgt
     6361 tgatgcacca cgagatcggg gacgccggcc tcggcctgag ttcctggggc taccccaggg
     6421 gcgggatggg cgccgtctcg gacgcgctca ggagctcggc cgaagagttc ggtgccgagg
     6481 taaggacgaa cgcccccgtg gagcgcatcc tgcagaaaaa tgggcgtgtg acaggtgttg
     6541 ttctggaggg cggggacgag ctggaggcgg acatcgtggt ggcggccacg catccgagga
     6601 ccaccttcct ggagcaactg cggccggatg agttgcccgg ggagttcgtc gatagcatag
     6661 agcggtggaa ctcacgcagc ggggtcgtca agatcaacct cgctctctcc gagctaccgg
     6721 acttcacctc gaaccctggc aagggcgacc atcttggtgg ggccatcgag cttgctcact
     6781 ccatcgacta catcgaggag gccttccagg atgcgcggcg tggcgagcca gcccggcggc
     6841 ctttctcgga cggtgtgata cccacggtgt tcgaccgcac gttgtgcccg gagggctacc
     6901 acatcatgtc gctcttcaca cagtgggtgc cgcacgaatg gtcggaggag ccgcaccggg
     6961 cggagcttga ggcgtacgcg gaccgcgtgg tcgacggcta caccgagctc gcgcccaatt
     7021 tgaaggatgc gatcctgcac cgtcaggtga taggaccgta tgacatggag caggagatcg
     7081 ggctcttcgg gggcaacatc ttccacggcg agctctcggc cgagcagctc ttccacatgc
     7141 gtccggcgcc aggctacgca gattaccgga cccccatacg tggcctgtac caggcctcct
     7201 cggcgacgca cgccggtggc ggtgtctgtg ccatcccggc ctacaactgc gtgcgggaga
     7261 tccggcgcga catgcgaagg gagcgtataa aggaacgcct gaccccccgc agcagaggat
     7321 agaatcgtgg cgctcgacgg cgctaccagg gccatgctgg gggcgcgctc cgtcgccgtg
     7381 gtcggcgcga gcccgaagtt cgactcgcca ggcaaccaca tggtacgcca gctcgcggtc
     7441 ggcggcttct ctggccgggt cgccgccgtc aaccccaggt acgacgaggt cgagggcttc
     7501 tcttgtttcc cctcgctcgg tgcgctgcct ttcgcccccg acctcgtgtt gcttggggtt
     7561 ggcaaccacc ggctcgagga acagatggag gcggcggcaa gttgcggcgc ccggggcgtc
     7621 gtggtgttcg ccagcgcgct cgaggccccg ccgcgtgacc ccccgctcgc cgagcggttg
     7681 aggcgcatcg ccgagggggc cggcatggcc gtgtgcggcg ccaactgcat ggggttcctg
     7741 gatgtcgagc gggggttgag ggccctggcc ttcgaggagc gtacggacct gaaaccgggg
     7801 aacgtggcct ggatctccca ctccggctcc gccttctctg cgctcttgca cgccgagagg
     7861 ggaatccact tcaacctcgc ggtctccacg ggccaggaac tccgtacgac catggccgac
     7921 tacatgctct acgccctcga acgagactcg acgcgtgtga tctccctgtt tctggagacc
     7981 atcagggacc cggaccgctt ccggctggcc ctcgcaactg ccgccgaacg ggacgtgccg
     8041 atcgtcgcgc tgaaggtggg aagagaccca gcctcacgcc ggctcgtcgc cgcccactcc
     8101 ggcgccctgg ctggggacga cgctgcttac gaagcgctct tcgatgccta cgatgtggcc
     8161 cgcgtcggaa cgctgaacga gatggcggac ctggttgaac tcctggccgc gggacgccgc
     8221 gccgcccgcg gaggcctggc cgcgatactc gactccggcg gcgagcgggc gcacctggtc
     8281 gactcggcgg caggcctccg ggttcccctg gcgcagccct cggagaagac tttgaaaagc
     8341 ctggccgcgc ggctggaacc cggactaccc tcggtcaacc cgctggatgc ctggggcacg
     8401 gacgacggcg cttacgccac cttcctggac tgctcccggc acctggcggc agaccccgac
     8461 acgggggctt tcgcctacgt ggtcgacctg cacagcgagc gtgccgaaag gggccacgcg
     8521 tgggccgcgg agagggtctg gagcgcgacg gacaaacccg tcgcggtggt ctgcgggtta
     8581 acgagcgcga tccaggatag cgcggcggac cagttgcgct ccgggggaat acccgtgctc
     8641 gaggacgtga actccggcct gctcgccttc cgccacctct tcgaccgacg cgactcgcgc
     8701 gccctggcct ccacgccgcc accggatgcg gtccccgacc acatccggct ccgctggcgt
     8761 gaaaccctgg cctccggccc acgccccgga gaggacgagg cgctggagat gctcgcggac
     8821 tacgggattc cggtagccca gaccgtggca gtggatagcc ttgatggagc cctcgcttcg
     8881 gcacagcgtt tgggataccc cgtggccctc aaaacagccg cagcatctca caagaccgac
     8941 gtcggcggcg tgaggcttgg gatagaatac ccggcggccc tgacggccgc ctacggggag
     9001 atgtcttctc gcctcggtcc cgaggtggtg gtgcaggaga tggtgggacc cggcgtggag
     9061 atggcgctcg gcatcgtcca cgacccccag ttcggcccgc tggtgatggt tgcagccggg
     9121 ggcatcctga tcgagacgct agcagaccgc cgcctggccc ttccccccct gaacgaggca
     9181 cacgccagaa ggctggtaga ccgtctcggc gcggtccgct tgctggacgg cgtcaggggt
     9241 gggccaccag cggacaggta ttcgctggcc cacgccctgt cacgcctctc cgttctggcc
     9301 cacgacctcg gggacctcat cgacgccctc gacgtaaacc cactcatagt cggggaaaaa
     9361 ggctgcacgg ccgtcgacgc cctggtcgaa ccgtcaacta ccggtactcg agagctgaca
     9421 gctgacggct gacggcttat cggtattggc tgggggtgcg ctctggcgcg gtccagtcgg
     9481 gtatctgcac gaccccgccc tccaggtatc cccgggcgac cataccgacg aacttgccca
     9541 ccgtggactc ctcgggggag aggggaccct gacggtagcc gcgggatgat gcgccccgct
     9601 ggacgccctc gcaggcggcc cagtcctggc cgttggtaat gtgccagaag tcgctggcgt
     9661 aagaaggatt gaaaccttct tgctccgtgg cctcgcggga gaagagccac tcgcactcca
     9721 cggcggtcct gtcgggtgtc aggggcctca gcctgtgggt catgatgtag tcgggatgga
     9781 ggctcacgag caggtttggg aagagctgga agtagtagac ttccctctcc tgcccttcag
     9841 ccaggtgtgg caacctcggg gcgtggctcc tgccgtcaag cgacatggtc tcggcgaagt
     9901 cctcgagctc catgggtccc ccgacccagg cgccggtaga ctcgtaatcc tccccgctct
     9961 tgggcgggct caccttgcac agctcggggt gtatggacgg gcagtggtag cactcgtggt
    10021 agttctcggc caggatcttc cagttcgccg ctacctcgta ggattgcccg gcggcggtca
    10081 cgagctcgcc ggggccgtgg tgggagattc tgtccccaag gtccccgacg tgatcctcga
    10141 acgacggggc atccccgctg gcgttgagga agacccaccc gtgccactcc gcaagacgga
    10201 ccggcaagag cgagtaatcc tccttgtcga acccgaagct cccgccgaac cccggcgtgc
    10261 cctgcaacct cccgtcgagg ccgtaggccc acgcgtggta ggggcagcgg ataaactttc
    10321 cgcccgcgct gcaaccgacc tccaacaggt cgtggccccg gtgacggcac acgttgtaga
    10381 aggcccgcaa gaccccatcc tcgcccctga cgagcaacgc ccgctcgtgc ccgagggcta
    10441 ccgctttacg gtcgccgggg ctccgcaaat cctcagaacg gcccacgcac acccagctct
    10501 cgtcgaagaa gtgctccatc tcccaggcga gcaccgaatc ctccgtgtag gccgcccgcg
    10561 gcaggctcat ctgcccgtca ggcataaccg acgagaccaa cagttcgcga tctagaggcg
    10621 tcacttggcc cccctttcga gccgatattc tgaccccggc ttgcttgttt gccgcaatct
    10681 aacagattca gtataatctt ttttaaatgg ctgttcaaga aacactcgag gctctgtaca
    10741 ccgttgcctg tggaatcgag gtggtagggt ccgttacgtc tggggtagga ccatatgggg
    10801 agaccagagc ggtgtttgcg ggcgtctatc cgggttcggc gtccctggtg tggcaggggt
    10861 gagaaccaga ggcaactaaa ttacggttca acgctgtacc tggaatgtgg gtagctagcg
    10921 aagaggagga aagggagatg ccggtctacg atgtggtggt ggtaggtggg ggcaacctgg
    10981 gtctatggac cgcttaccgg ctggccaggc gcgggttcgg gcgggttgcg gtgctcgagc
    11041 gcggctgggc cggtggcggg gctaccagcc gctcggccgg ggtggtccgt cagcagggag
    11101 gctcggagac ggcggccagg cttggcaagc tctccaggca gctgtacctc tcgctcgggg
    11161 aggaactcgg cctcgactcc ggtttcaggg agaccgggta ctacatcgtc gccgagacgg
    11221 aggaggaaaa agaagggttc atgcgcctga tcggggtgcg ccgcgaggcc ggggtagaga
    11281 acgagtgggt cacgccggag gaggggagaa ggcggtttcc cgacctcgcc tgggaccgtt
    11341 tcgtgggggc cacctacacc gcaggtgacg gctacgtcca tccgcccatc gcggcccgca
    11401 acgccacctg cgccgtacta tcggaggagg ccgtggagct gttcgagatg tgcgaggtgc
    11461 tcggtataga ggagcgcggg gatcattacg ccgtggagac cgctggtggg ctcttcgagg
    11521 cggagagggt ggtcgatgcc ggcgggcctc ggggggcgca cgcggtcggt gccatggttg
    11581 gggtggaagt gcccgtcatg gccgtgcggc atcagatcgt caccttcccg gtgctcggtg
    11641 gtgatgccat tcacccgttc ccgctcgtct tcaacgtggg caaggggtac tactggcggc
    11701 ccgaggagca gggcgcgctg ctcggcatga gcaaccccgt cgacgaggcg gacacctcgg
    11761 gacggtacca gatcggcttc gactgggact actacgaaaa gatgcgcccc gactgggagt
    11821 cggcacatcc cgcgctagcg gggctgcccg tctcacgggc gtgggccgcg tccatagact
    11881 atacgcccga ccacctaccg atagtcgacc agcccaggga agggttctac gtgctggccg
    11941 cgggcgggca cggcatgatg tggggtcctg cgctcgggga ggccatggcg cgtttgatct
    12001 ccgaggacac cctcggggat ttgccgaaag aagacgtgga gctgtcgcgg ttctcgaggg
    12061 agaaggaccc gaacaggctt caggatgcga tcgcgcttcc cttcccgaag agatagagga
    12121 gacagagcca tgacgacggt gccggagcaa cagatcttgc ccgagatccc cgaagaggtg
    12181 agggagttga aggatcgcgc cgcgcggttc gtgacggaga agctccatcc cttcgaggtg
    12241 gagatagccg agagggggga gatcgacgaa gacaggctga gggctttgcg cgacgaggca
    12301 agggcggccg ggctcagcaa cctcaacatg ccaaccgagt acggtgggat ggacctctcc
    12361 atggcctcgc tggtggcgct ggaggaggag tcgggcaaga ccaccaacgg cctcggctac
    12421 atggtcgccg accgtggtcc gcgggagctt ctcgagctcg ccagccccga gcaggtagag
    12481 cgctacgtct tgcccgtcgt gcggggtgag actcgggagg cgtgggccat caccgaaccc
    12541 gaggccgggt cggacatcca ggacataaag actaccgccc agcgggatgg ggaaggttgg
    12601 gtgctgaacg gggagaaatg gttcgtgacc ggcggggagc gtgccggctt cttcatcgtg
    12661 cttgcctggg agggtgggga gcaggcatcc tttctcgtgg acagggatac tcctgggctg
    12721 gagatcgtac gcaccccgac cttcatgcac gacccttacg cctccaaaca cgtcgagctc
    12781 aggctcgatg actgtctggt tccggctggc gcaaagctgg gcagtggggg cgatgagggg
    12841 accaaacgct ggttctcgct ggagaggttg atgatcgccg cccgctgttg cggggccgcc
    12901 gagcggctgg tagaccttgc ccgctcctgg tcactggagc gttcggcctc gggcaggaag
    12961 ctcgcggagt accaggccat ccagttcatg ctggctgact ccctgacgga gctgaacgcg
    13021 gccaggctca tgacgtacca cgtcgcccgc tcgtgggaga gggaagacgc caggatcctg
    13081 cacggcaaga cggccatggc gaagctcttc gcctccgaga tggccggcag ggtggcggac
    13141 cgggctgtcc agaccttcgg cggtcgcgga tacatgaccg agaacccggt cgagcgctac
    13201 taccgcgagc tcagggtgga ccgtatctgg gaggggacca gcgagatcca gcgcaccatc
    13261 gtggcccgcg gcatgctcaa gcggggcgcc gaagcctaca tgatgaacga gtagcggggg
    13321 tacttcgtgg acgtatcgat ggccagagag aagatcttta tcgacggaga gttcgccgaa
    13381 ggcgcggacg gcgagtggcg aaaggtgctg aaccccgcga cccgggaggt catcgccgag
    13441 gtgcccgagt gctctgggga ggatgtggac agggccgtag gggctgccag gaaagccttc
    13501 ggagggtggt tcgattccac gcccgcagag cggtcgcgga tgttgcacga tctcgccgac
    13561 gttctggaag agaacgccga ggagcttgcc caactggagt cgcgcaacgt gggcaagccc
    13621 atcagctcgg cgcgggagga gttgccttac atcgtcgaca acctgcgttt cttcgccggg
    13681 atggcgcgcg acgtcgaggg caagtcggcc ggggagtaca cgcgcgggta caccagcatg
    13741 atccggcgcg aacccgtagg cgtcgtcggc cagatagcgc cctggaacta ccccctcatg
    13801 atggccgtct ggaagatagg gccggccctg gccgcgggca acaccgtggt gctcaaaccc
    13861 tcagaacaga caccgctcac gacccttagg atggcggagc tggctgccga catctttccg
    13921 cgcggcgtcc tgaacgtcgt aaccggtgac ggcgagccgg tcggcgcggg catcgtgcgc
    13981 catcctgggg tggacatggt ctccctgacc ggtgacgtgg ccaccgggaa agcggtcgcc
    14041 agggccgctt cggacacgct caagcgcgtg cacctcgagc tcggcggcaa ggcgcccgtg
    14101 gtggtcttcg acgacgcgga ccttgaggag gtcgtagctg gcatccggta tgccggctat
    14161 gccaactccg ggcaggactg cacggcagcc gcgcgcgtca tcgccggacc tggcatctac
    14221 gaagaggtct cctccagg
//
LOCUS       DBNGVD010000498         7681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_669791, whole genome shotgun sequence.
ACCESSION   DBNGVD010000498 DBNGVD010000000
VERSION     DBNGVD010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_669791"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="AC16CM_13430"
     CDS             <1..1054
                     /locus_tag="AC16CM_13430"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR3140880.1"
                     /translation="GATTSLFDTEIYANDAPFGGGLGAYGVTTIERSAIYLNRAVPGG
                     LGGGIRAAGSSSVTTLTNVTMAHNQAGGSGAAIQARNGPGFTLNNVTIARNTADSDGD
                     GSGNAAVTLRRDSSTTSMTLRNTILAANEDGSPGSSDVPDCEVQGGATITAASYDLIG
                     NSDGCNLTIPVGAPGIHVGSAANPVDPKLDIGINLNGGLTSTLPLLPGSPAINAASEA
                     ESGAEACEPNDQRGVPRADCDMGAYELVRCRDAVVNVVGTAGGDTLLGSGRRDSILAL
                     AGADEVFGNGGDDVICGGEGSDTIRGGGGNDRLFGQDRGDTLVTRDGVRGNDLANGGD
                     GIDICRTDAEDRKASC"
     gene            1237..1566
                     /locus_tag="AC16CM_13435"
     CDS             1237..1566
                     /locus_tag="AC16CM_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140881.1"
                     /translation="MTGDALIQEINTAYRRLGAAMEDMARADRELANHVRRVRLDNAE
                     AILEARNERTASLYLDGLLDTEEHRDLEAARSWAEVGLQQARFEVDRLQLIVRLLGAR
                     AGGATRG"
     gene            1563..2099
                     /locus_tag="AC16CM_13440"
     CDS             1563..2099
                     /locus_tag="AC16CM_13440"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR3140882.1"
                     /translation="MSGREVHVYTDGASTGSRGPGGYGAVISYRRGETVEISGGERET
                     TNLRMEITAACAALESIGEGHTVTVYADASYLVNCMRRGWHEKWRKNGWLNYRKQPVA
                     NRDLWERLLRATARHGEVRWKKVKGHSRTAGAHKNGNDRADELAVAAKKRVGKTLETG
                     GNRAPDGSTASRSTSSRL"
     gene            2450..3370
                     /locus_tag="AC16CM_13445"
     CDS             2450..3370
                     /locus_tag="AC16CM_13445"
                     /inference="COORDINATES: protein motif:HMM:NF042637.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARPP-1 family domain-containing protein"
                     /protein_id="HIR3140883.1"
                     /translation="MDEILQRTLYGVDIGAPISHGLLHVFPLRGCMPEEDGLSLLEDG
                     LLAGTLRIEGLHEAGCAQGLRVINEGDLRVLILEGDELTGAGQHRVANSSVLIDAGSE
                     LTLPVSYVERGRWGYRSRAFFPATGSPHLTLRRLASRPVHYSVKSGRSLWDGQQAVWR
                     DLDHVARRYKTGLPVRALRASRYSHAERLDAFEKLARELPEETSGVVVAIGERLALLE
                     ILAGPRTFARVFRKLLSRYAFESVGLDRPYGTPEVSEVRDFIQTVAETTHGEQRAAGA
                     GRDVKLKGDEISGYALIGQKGVLHAAACSE"
     gene            3578..3907
                     /locus_tag="AC16CM_13450"
     CDS             3578..3907
                     /locus_tag="AC16CM_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140884.1"
                     /translation="MNEPRNLAFGEMEWTDDAPGIRAKEADVGGKRWATVEYGEGVRR
                     DDWCEEGHRGYVLSGEIEYEFDDGRDPLRAAQGEAFLLPPADLGSGAHRGRNIARETT
                     RLFLIDD"
     gene            3909..4466
                     /locus_tag="AC16CM_13455"
     CDS             3909..4466
                     /locus_tag="AC16CM_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3140885.1"
                     /translation="MTTPRRHLDEPSVSIPDPAALVDAVLERSTGKNSNIHGQDHWMR
                     VGAAGLALLPETPEADPSLLFLFALFHDSMRVNDSIDPMHGPRAAVLARELRGGPFDL
                     GDEEMELLAFACEEHTNGKLGDEGTVGVCWDADRLNLWRVGFEPNPRFLSTEAARSRE
                     RIAWARDLQQESFTWEQIFRACGLG"
     gene            4672..5631
                     /locus_tag="AC16CM_13460"
     CDS             4672..5631
                     /locus_tag="AC16CM_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140886.1"
                     /translation="MEDLARRGFSGVLHTFSENDFNYYRDQMRRIVEVSHEVGLEVQV
                     NPWGLGHAFGGEAESLFVATHQEVSQVFDDGQSVGAGCPNRPEFRAFVRSWADAALET
                     GADRIFWDEPHWALPTQFGLPGDRWSCWCDLCRQLFHERFGGPMPTTLTPEVIAFRED
                     SLVDFLRELVGHVYAQGGRNTVCLLPLTEGPLGLPDWSKVASMPGLDTLATDPYWQAF
                     GRPVREFVSDFAGLVGSLGERYGVETEIWIQGFGLGPEDVPDIQTAVEAARAAGVENL
                     WTWGYEACGHMDHLDTREPEVVWAALTEALTSGGNDHRQERRV"
     gene            5684..6805
                     /gene="nagA"
                     /locus_tag="AC16CM_13465"
     CDS             5684..6805
                     /gene="nagA"
                     /locus_tag="AC16CM_13465"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3140887.1"
                     /translation="MLAPQPLGRAAVVFGEGRILDVVRDPRILDLPPDVREVEGFICP
                     GFVELQINGSFGLNVGPDGEALKHLARELPKTGTTSFLPTLVSSPEHTYPRFLDALSG
                     VRDVPGARILGAHLEGPFLSPLRKGAHDPANLLPIDLGLIRTLAGSGLLSMMTLAPEL
                     PRAREAIRLLLENGVVPSAGHTDATYEELTKAVDAGLGTGTHLYNAMSPLGHRAPGAV
                     GALLSDHRTRVGIIADGVHVHEAALEIAYLQKGPDALALVTDAMEAAGMPEGEYELGD
                     RKVRLEGNAVRTPEGSLAGSVLTMDAALRNAAGFLNIPLHEALRMITSTPAAVLGLPA
                     RGKIAPGADADLVVLSAGGEVLETLVAGRRVYGPPEEPA"
     gene            7200..>7681
                     /locus_tag="AC16CM_13470"
     CDS             7200..>7681
                     /locus_tag="AC16CM_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enolase-like domain-containing protein"
                     /protein_id="HIR3140888.1"
                     /translation="MSRIAYARTYQVAYPLDEPLSDSIHYMPVRAALLVELGTDDGKV
                     GIGESAIYGGPASVVETMIHEELVPRILGEDPTRPEWLWQVMMGRSHQHGDGGVLPAA
                     VSGLDIALWDLMGRTSGLPLHRLLGGYRDHVRAYASAGFYAEGKDAQGLADETRRYVE
                     A"
BASE COUNT         1429 a         2405 c         2606 g         1241 t
ORIGIN      
        1 gggggcgacc acctcgctct tcgacaccga gatctacgcc aacgatgccc ccttcggcgg
       61 tggcctcggc gcctacggcg taacgacgat cgagagaagc gcaatctacc tcaaccgtgc
      121 ggtccccggt ggtctgggtg gcgggatccg ggccgcaggc tcctcctcgg tgacgacgct
      181 cacgaacgtt accatggcgc acaaccaggc cggcggcagc ggtgcggcca tccaggcccg
      241 taacggtccc ggctttaccc tcaacaacgt cacgatcgcc cgcaataccg ctgacagcga
      301 cggggatggc tcgggaaacg cggcggtcac cctcaggcgc gacagctcta cgacttcgat
      361 gacgcttcgt aacacgatcc tggcggccaa cgaggacggc tcgcccggta gtagcgacgt
      421 gcccgactgt gaggtgcagg gtggagccac gatcacggcg gcgtcctacg atctcatcgg
      481 caatagcgac ggttgcaacc tcacgatccc ggtcggcgcg cccggcatcc acgtcggtag
      541 cgcggcaaac ccggtcgacc ccaagctgga catagggatc aatctcaacg gcggcctgac
      601 ttccacgctg cccctgctac ccggcagccc cgcgataaac gccgccagcg aagcggaatc
      661 cggagccgag gcctgcgagc ccaatgacca gcgcggtgtg ccccgcgccg attgcgatat
      721 gggcgcctac gagctggtca ggtgccgcga tgcagtggtg aacgtggtcg gcaccgccgg
      781 aggagacacc ttgctcggca gcggcaggcg tgattccatc cttgccctgg cgggcgcgga
      841 cgaggtcttc ggaaacggcg gcgacgacgt gatctgcggc ggagaaggaa gcgacacgat
      901 ccgtggcggg ggaggaaatg atcggctctt cgggcaggac cgcggagata cgctggtcac
      961 cagagacgga gttcggggca acgacctggc caacggggga gacggcatag acatctgcag
     1021 gacggatgcc gaggacagaa aggcaagctg ctagcccaag aagcagcctg tcaggtcaga
     1081 agccgggtgg tgggaagaca ccccgccacc cgtaggcatg gctccggaag agtgcgccaa
     1141 gggctcctcc gcctctcgtg tgcttgaccg caaagcgttc atactgtctg tcatcgaggt
     1201 atcgaagttg gccggttggg aaaggaggag acactgatga cgggagatgc gcttattcag
     1261 gagatcaaca cggcgtacag gcggctgggt gccgcgatgg aggatatggc gagggccgac
     1321 cgggagctcg ccaaccacgt cagacgggtc cgcctggaca acgccgaggc catcctggag
     1381 gcgaggaacg agcggacggc cagcctctac ctggacggcc tgctcgatac cgaggagcat
     1441 cgtgacttgg aggccgcccg gtcctgggcc gaggtcggcc tgcaacaggc caggttcgag
     1501 gtcgaccggt tgcaactcat cgtccgcctg ctcggggctc gggcgggcgg ggcgacccgc
     1561 ggatgagtgg gcgagaggtc cacgtctaca ccgacggcgc ctccacgggc tcccgaggtc
     1621 caggaggcta cggggccgtt atctcgtatc ggaggggcga gacggtggag atcagcggcg
     1681 gagagcgcga gaccaccaac ctgaggatgg agataaccgc ggcctgcgcc gccctggaga
     1741 gtatagggga ggggcacact gttaccgtct acgccgatgc ctcctacctc gtcaactgca
     1801 tgaggagggg ctggcacgag aagtggagaa agaacggctg gctaaactac cgcaagcagc
     1861 ctgtggcgaa cagggacctg tgggagaggc tcctgagggc gacggcgcgc cacggggaag
     1921 tgcggtggaa gaaggtcaag gggcactcca ggaccgcagg agcccacaag aacgggaacg
     1981 accgggcaga cgagctagcc gtagccgcca agaagcgggt cgggaagacc ctcgagacgg
     2041 gcggtaaccg ggcgcccgac ggctccaccg catcaagatc cacgtcctcc cgtctgtagt
     2101 cccgtccacc cccgagatta cggtaggtgc gtcgggagct agaacaggac gtacctgaac
     2161 caggaattgg gaacatctgc ctgcccttgg tcatccagca aaggtgtgcg tacgtaaatg
     2221 tcaacaggag ggtctatgag aagtggccga cgacccacgg ccatacgcat cgagattttg
     2281 tatcaggggg cggtgcgagc ggcatagata gaccaaagag ctggtagatc atggagaggt
     2341 gcccgccgga cggcgtgatc tctccttctt cccatataac tccgggtgag cgcctggagt
     2401 atcccaggcg cccagacccg atccccctat tagaaggagg gagcgaatct tggacgaaat
     2461 tttgcagcga actctctatg gtgtggatat aggcgcccca atctcccacg gcctcctgca
     2521 cgtgttcccg ctacgtgggt gtatgcccga ggaggacgga ctctcgcttc tggaagacgg
     2581 gttgctggct ggaactttgc gaatcgaggg cctacacgaa gccggatgcg cgcaggggct
     2641 acgggtcata aacgagggag acttgcgggt cttgatcctg gaaggagatg agctcaccgg
     2701 cgccggacag caccgcgtgg cgaactccag cgtcctcatc gatgccggct ccgagctcac
     2761 cctgccggtc tcgtacgtcg agcgtggacg ctggggctac cgttccaggg ccttctttcc
     2821 ggcgacgggc agcccgcacc tcacgctcag gcggcttgcg agcaggcccg tgcactactc
     2881 cgtgaagagc ggcaggagcc tgtgggacgg ccagcaagcc gtgtggcgag atctggatca
     2941 cgtggcccgc cgctacaaga cgggcctgcc ggtgcgtgcg ttgcgtgcct ccagatactc
     3001 ccacgccgag aggctcgacg ctttcgagaa gctggcccgc gagctaccgg aagagaccag
     3061 cggcgtggtc gtagccatcg gagagcgcct ggcgctgctc gagatactcg ccggtccccg
     3121 caccttcgcc agggtcttcc gcaagctgct ctcgcgctac gcgttcgagt ccgttggcct
     3181 ggatagaccg tacggtacac cagaggtttc agaggtcaga gacttcatcc agaccgtcgc
     3241 ggagaccacg cacggggaac agcgggccgc tggtgccgga agggatgtca agttaaaggg
     3301 agatgagatc tccggctacg ccctcattgg ccagaagggc gttctgcacg cagccgcctg
     3361 ctcggagtag cctccaactg tcgccagccg aaggaagcag taaggtctca gccacgaacg
     3421 ggggctgaaa ccagaccgct cgtccctaac aacctcggtt cgttcgcaaa ctgctctata
     3481 ctttctgggt cgagaaggga gcgggcctgg tgtggtgccg caggggtaag ccttcccaat
     3541 cgaaagaacg tcagccgaga aatgtgggga gtggaacgtg aatgagccgc gcaacttggc
     3601 ctttggcgag atggagtgga ccgacgacgc gcccggaatt cgagccaagg aagcggatgt
     3661 ggggggcaaa cgctgggcca ccgtggagta cggagaagga gtccgtcggg atgattggtg
     3721 cgaggagggt caccgcgggt acgtcctgtc gggcgagatc gagtacgagt tcgacgacgg
     3781 ccgcgacccc ttgcgcgccg cccagggtga ggccttcttg cttccgccag ccgacctggg
     3841 aagtggggca cacagggggc gaaacatcgc ccgcgagacc acccgtctct tcctgatcga
     3901 cgactagctt gactactcca cgacgacacc tcgacgaacc cagcgtctcc atcccggacc
     3961 ccgcggccct ggtcgacgcc gttctcgaga ggtctacggg caagaactcg aacattcacg
     4021 gccaggacca ctggatgcgg gtcggcgccg cgggcctcgc cctcctgccc gagacgcccg
     4081 aggccgaccc gtctcttctg ttcctgttcg ccctcttcca cgactccatg agggtcaacg
     4141 acagcatcga ccccatgcac ggcccgaggg ccgctgtcct ggccagggag cttcgcggcg
     4201 gtccgttcga cctgggggac gaggagatgg agctcctggc gttcgcctgt gaggaacaca
     4261 ccaacggcaa gctcggcgat gaggggaccg tcggggtgtg ctgggacgcc gaccgtctca
     4321 acctgtggcg ggttggattc gagccaaacc ccaggttcct ctcgaccgag gcggcaagga
     4381 gcagggagcg catagcgtgg gcgcgcgacc tgcagcagga atcctttacc tgggagcaga
     4441 tctttcgggc ttgcgggctc ggctgacgcg tcgaccgagg gggagacggg tcagccggcg
     4501 ggcgcttatc cggtcccggc cgggtggtag tgtaggcggc aggacgttgt tcgggctagc
     4561 gggaggaaag gggtggccgg ggcgatccgg gacaccccga aagggaggta gaacatcggc
     4621 acgggagcat cttacttcgg caaccgaata ctccgtcacg tcgcctcgga catggaggat
     4681 cttgcccgac gcggcttttc cggcgtgctg cacaccttct ccgagaacga cttcaactac
     4741 taccgggatc agatgcggcg catcgtggag gtctcgcatg aggttggcct cgaggttcag
     4801 gtgaacccct ggggcctggg tcacgccttc gggggcgagg ccgagagcct cttcgtggca
     4861 acccaccagg aggtctccca ggtgttcgac gacggccagt ccgtcggggc cggctgcccg
     4921 aaccggcccg agttccgggc ctttgtccgc tcctgggccg acgcggcgct cgagaccggg
     4981 gctgacagga tcttctggga cgagccccac tgggcacttc ccacccagtt cggccttccc
     5041 ggagaccggt ggagctgctg gtgcgacctg tgccgacaac tgttccacga gcgattcggc
     5101 gggccgatgc ccaccaccct gacccccgag gtcatcgcct ttcgggagga ctccctcgtt
     5161 gactttctgc gcgagctggt cgggcacgtc tacgcgcagg ggggacgcaa cacggtctgc
     5221 ctgcttccgc tcaccgaggg gccgctcggc ctcccggact ggtcgaaggt ggcgagcatg
     5281 cccggcctcg ataccctggc caccgacccg tactggcagg ccttcggacg cccggttcgc
     5341 gagttcgtca gcgacttcgc cggtctggtc gggagcctcg gagaacgata cggtgtggag
     5401 accgagatct ggatccaggg tttcggcctt ggccctgagg acgtcccaga catccagacc
     5461 gccgtcgagg ccgccagggc agccggcgtg gagaacctgt ggacctgggg ctacgaggcg
     5521 tgcgggcaca tggaccatct cgatacccgc gagcccgaag tggtctgggc ggcgctcacc
     5581 gaggccctga cctctggtgg taatgaccat cgccaggaaa ggcgcgtttg acccggaagc
     5641 ctcccggaca ccacccgtga cgccccgggc tcttctaggc aacgtgctcg ctccgcagcc
     5701 tctgggccgc gccgccgtgg tcttcggtga aggaagaatc ctggacgtgg tccgtgaccc
     5761 tcgcatcctg gacctgccac ccgacgtgcg agaggtggag ggttttatct gcccggggtt
     5821 cgtcgagctg cagataaacg gctccttcgg cctgaacgtc ggccccgacg gggaagccct
     5881 gaaacacctg gcccgcgagc taccaaagac gggcaccacc tcgtttcttc ccacgctggt
     5941 ctcgtcgccg gagcacacct acccgcgctt tctcgacgcc ctctccggcg tcagagacgt
     6001 tcccggagcc aggatactcg gcgcccatct ggaggggcct tttctatccc cgctgcgcaa
     6061 aggagctcat gaccccgcca acctcctgcc tatcgaccta ggcctgatac ggacgctcgc
     6121 aggatccggc ctcctcagca tgatgacgct ggcgccagag cttccacgtg ccagggaggc
     6181 gatacggttg cttctcgaaa acggtgtcgt tccaagcgcc ggccacaccg acgccaccta
     6241 cgaggaactc acgaaggccg ttgacgctgg gctcgggacg gggacccatc tctacaacgc
     6301 gatgagcccg ctggggcacc gtgcccctgg ggcggtgggg gcgctgctct ccgaccacag
     6361 aacgcgcgtc gggataatcg cggacggcgt ccacgtgcac gaggccgccc tcgaaattgc
     6421 ctaccttcag aagggtcccg atgccctggc cctcgtcaca gacgccatgg aggccgccgg
     6481 catgccggag ggcgagtacg agctcggcga tcgcaaggta cggctcgagg ggaatgctgt
     6541 cagaacgccc gagggatcgc tggccggaag cgtcctgacc atggacgccg ccctccgcaa
     6601 cgcggcaggg ttcctgaaca tacccctgca cgaagccctg cgcatgatca catcaacccc
     6661 ggccgcggtc ctcggcctac cggccagagg caagatagcc ccaggcgctg atgccgacct
     6721 ggtcgtcctg agcgccgggg gagaggtgct ggagaccctg gtggccggca gaagagtcta
     6781 tggtcctccg gaggaaccag cgtgatccgc gacggccgtt ctgggtgggg ccggaagtag
     6841 ccatccgaga accacccgat ctatccgccc tacttcgcga cggtaagctt ctgcggatgt
     6901 cggatccccg gcattccagg cggtaggttc ccctgcccgg agtcaaaccc ctcggcgatt
     6961 tgcaaggctg gagacgctac agcgtggacc tgggtcatcg cgagagcttg tggtgtgtag
     7021 cgggagcgtg cgccggttgc tacgattctt ctccagaata ggcggatcga aggcaggatc
     7081 acgtcatagg gaagatatag cacgggtgcc gggggaggcg tgggtgctct gttgtcagcg
     7141 agtgtcttcg aaattgcttg cgtactcgaa gaacgacagg tgaagaaagc gaggatcccg
     7201 tgtccaggat tgcttacgcc agaacctacc aggttgcgta cccgctggat gagccacttt
     7261 cggattccat acactacatg ccagttcggg ctgccctcct ggtggagctg ggtaccgacg
     7321 acgggaaggt tgggataggg gagtccgcca tctacggggg gccggcctcg gtagtggaga
     7381 cgatgatcca cgaggaactg gtgccgcgca tactcggaga agaccccacc aggccggagt
     7441 ggttgtggca ggtaatgatg ggccgctctc accagcatgg cgacgggggt gtgctccccg
     7501 cggccgtctc cggcctagac atcgccctct gggatcttat ggggcggact tctggcctac
     7561 ccctccatcg cctgcttggt ggctaccgcg accacgtaag agcctacgcc agcgccggtt
     7621 tctacgcgga gggcaaggac gctcaggggc tggctgatga gacgcggagg tacgtcgagg
     7681 c
//
LOCUS       DBNGVD010000499         1834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4679909, whole genome shotgun sequence.
ACCESSION   DBNGVD010000499 DBNGVD010000000
VERSION     DBNGVD010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4679909"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16CM_13475"
     CDS             <1..281
                     /locus_tag="AC16CM_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3140889.1"
                     /translation="DEPTSGLHLADVEQLLGLLDRLVDSGKSVIVIEHHQAVMAHADW
                     IIDLGPGAGHDGGRIVFEGTPANLVAARSTPTGQHLAIYTGERSAISN"
     gene            complement(318..1094)
                     /locus_tag="AC16CM_13480"
     CDS             complement(318..1094)
                     /locus_tag="AC16CM_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140890.1"
                     /translation="MSATLMYVGIGAVLATLVGIFAIAILFLQTARRYVDLTEKRLES
                     MREREDFLLKIVQRQGRALEEGLQREAEQRESIPEMAGYAAGTRQAEEPTGTRPPENP
                     RRRGGRERREQPPKEEHAQQEKTGETASRQENDAPLRGVQVPHPDDDAPGRGWNGSPA
                     RFFQKCYDRYLEHYEGYVRLAERLHATHDEAGTDERARGEWEDKLRRAYDAIERTTQR
                     LDVLEGHYPELATDSDRISSRIGTARLHADLAARFGRPRP"
     gene            1359..1610
                     /locus_tag="AC16CM_13485"
     CDS             1359..1610
                     /locus_tag="AC16CM_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140891.1"
                     /translation="MAYFEGQLKRAETEPGEEHTGYVLETEIDVSAIQDVEELVGDQI
                     AVTGDVEIVDFPERGKILVFRATSVAVLEDEEEEEVTEP"
     gene            complement(1597..>1834)
                     /locus_tag="AC16CM_13490"
     CDS             complement(1597..>1834)
                     /locus_tag="AC16CM_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140892.1"
                     /translation="NLDAALGRIQDFEAILDGWRALNCTLGNAVRVRRSGETIEGRAV
                     DLTGEGALLLATPDGTVELFEGEIEQIVREPRVP"
BASE COUNT          319 a          596 c          598 g          321 t
ORIGIN      
        1 tagacgagcc gaccagcggc ctccacctag ccgacgtcga gcagctgctc ggcctgctcg
       61 acaggctcgt cgactcaggc aagtcggtca tcgtaataga gcaccatcag gcggtcatgg
      121 cccacgccga ctggatcatc gacctcggac caggagcggg ccacgacgga ggccggatcg
      181 tcttcgaggg aacgcccgcc aatctggtgg ccgcccgctc cacccccacc ggccaacacc
      241 tcgcgatcta cacgggagag cggtcagcta tcagcaacta gcggtcagcc tcagccacaa
      301 tagctgaatg ctaacagcta ggggcgcggg cgcccgaagc gcgcggcaag gtcggcgtgg
      361 aggcgggcgg tgcctatgcg ggaggagatg cggtcgctgt cggtggccag ctcgggataa
      421 tgcccttcca gaacgtcgag cctctgggtg gttctctcta tggcatcgta ggcccggcgc
      481 agcttgtctt cccactcgcc cctggcgcgc tcgtcggtac cagcctcatc gtgcgtggcg
      541 tggaggcgct cggcgaggcg cacgtagccc tcgtagtgct ccaggtacct gtcgtagcac
      601 ttctggaaga acctggcggg ggacccgttc cagccgcggc caggcgcgtc gtcgtcgggg
      661 tgcggtacct gaacgccgcg tagcggcgca tcattctcct gccgcgacgc cgtctcaccg
      721 gtcttctcct gctgtgcgtg ctcctctttc ggtggctgct cgcgcctctc gcggccgccc
      781 cgccggcgcg ggttttcagg cggtcgggta cccgttggct cttcggcctg gcgggttccg
      841 gccgcgtacc cggccatctc ggggatgctc tcgcgctgct ccgcttcgcg ctgcaggccc
      901 tcctccaggg cgcggccctg tcgttgcacg atcttcagaa gaaaatcttc gcgctcgcgc
      961 atcgactcca ggcgcttctc ggtcaggtcg acgtagcggc gcgccgtctg caggaagagg
     1021 atggcgatgg cgaagatgcc gacgagcgtg gccagcactg ccccgatgcc aacgtacatc
     1081 aacgtcgcgc tcattcctct ctctttctcc tggcgtcgcc caatacaggt ccgaggggcc
     1141 catctgaaaa cgtattaaat cccaggtcag aaaagatagc aagcgagggt acccgcgtgt
     1201 gcccgggacg atagtccggg cctcgacaga ccatgatctc gttcggatcc tctcccggcc
     1261 tagtggcgtt ccggcagacg ctggcaagga gatctccatc ccgcaaccgc gaatacgggt
     1321 actatatcct cacatgaaat cgagagaggg gatcggccat ggcctacttc gaaggacagc
     1381 tcaagcgagc ggaaacggag ccgggagagg agcacaccgg gtacgtactg gagacggaga
     1441 tagacgtatc ggccatacag gacgtcgaag agctggtggg ggaccagatc gcggttacgg
     1501 gggacgtaga gatcgtggac tttcccgagc ggggcaagat ccttgttttc agggcgactt
     1561 cggtggccgt cctggaggac gaagaggaag aagaggttac ggaaccctag gctctcgcac
     1621 gatctgttcg atctcaccct cgaacaactc aacggtccca tcaggggtgg cgagcaggag
     1681 cgcgccctcc ccggtcaggt ccacggcccg gccctctatc gtctcgcccg agcgcctgac
     1741 gcggaccgcg ttgccaaggg tgcagttcag ggcccgccac ccgtcgagga tggcttcgaa
     1801 gtcctggatg cgcccgagcg cggcgtcgag gtta
//
LOCUS       DBNGVD010000500         6219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1035824, whole genome shotgun sequence.
ACCESSION   DBNGVD010000500 DBNGVD010000000
VERSION     DBNGVD010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1035824"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="truB"
                     /locus_tag="AC16CM_13495"
     CDS             <1..524
                     /gene="truB"
                     /locus_tag="AC16CM_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013660.7"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3140893.1"
                     /translation="PMVSALKREGQRLYDLHRRGITVEREPRPVTVHRLALTAFDPAE
                     KTADFEVSCSSGTYVRTLVADVADRAGSGAYLTALRRTAVGHLSVKDSSPPGALSPTT
                     LRNRIIPPAEVVSHLSAVVVSGEVRASVCHGVRMEACGIDGSYRVMCGEELLAVYRDE
                     GKEGRAEVVLCAG"
     gene            512..1408
                     /locus_tag="AC16CM_13500"
     CDS             512..1408
                     /locus_tag="AC16CM_13500"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3140894.1"
                     /translation="MRGVVVALGNFDGVHLGHQEVLRRAVEEGRRAGRQVVAATFRPH
                     PRAVLGAGDPPRLLSPMDVRREALVRYGADEVVEIPFDLELSRKSPEQFVRDVLVGDI
                     GAAVVVVGENFRFGYRAAGDFGDLDRLMREMGGEAVAVDVRAAGGGPDISSTRIRALI
                     AQGKVEEAAGLLGRAFVLRGEVVVGDRRGRTIGFPTANVLPDEEAVVPAYGVYAGFVL
                     VGDQEFVACTNVGVAPTFARTDSRVEAYLLDFDGDLYGRTVDVGFTHRIRGEKKFLGI
                     DELKEQIQNDVETARSLAKNGR"
     gene            1492..2439
                     /locus_tag="AC16CM_13505"
     CDS             1492..2439
                     /locus_tag="AC16CM_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR3140895.1"
                     /translation="MSRTRIRQGRKYWTVALVLLALAVPACANSGEETLSDSDEVARD
                     RADGVSRSQPEAGAGAPEPASTTQGGTAQGVSGAVVPPEDFDRKIIKSAEIGLTSKDV
                     RGEAAVAQQVVAGVGGTTVSSQTYRSGDTLYANLILSVPSEHFERALDEIRGLGDEVT
                     MDTVSGQDVTGEYVDLQSRERNLLAAEQSLLDLYNRAADVQEALSIQSELTEVRGEIE
                     QVQGRMQYLKQSSDMSRISVNIQPVSNPPKPPPAWDPAREVARAWNASLAVLQGVALA
                     VISTVVFGWWIIPPLALAAWWLRRRLRHESLTPRPTEPE"
     gene            2593..2859
                     /gene="rpsO"
                     /locus_tag="AC16CM_13510"
     CDS             2593..2859
                     /gene="rpsO"
                     /locus_tag="AC16CM_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3140896.1"
                     /translation="MAVAEQKAEIIQENRTHEGDTGSPEVQVAVLTRRIAHLTEHLRE
                     HKHDYHSRRGLLKMVGRRRRLLKYLQNKDVERYRALIAKLGLRR"
     gene            2896..4953
                     /locus_tag="AC16CM_13515"
     CDS             2896..4953
                     /locus_tag="AC16CM_13515"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3140897.1"
                     /translation="MRLEIPVGERSIVLETGKLAKQAGGAVVARLGDTTILSTATRSS
                     NPRPGATFLPLSVDIEERMSAAGKIPGGFIKREGRPSERAILTSRLTDRPLRPLFPKN
                     YHYEIQVIGTVLVADQETPFDTVSMVGASAALALSDLPFQGPIGAVRIGRSLDGEFVL
                     NPTYEQISESDLDLVVAGTKEAITMVEAGANEVPESVMVEALQLAQEIVQNQALAIEE
                     WAGEYGKEKQQIDGPEENTFVTSAREQYFDRVKEGIVQTDRRVRSTMLAEIREEMVEG
                     REEEEIPAITAALSTVEKEAFRELYLKDHKRTDLREMDEIRPTSAEVAVIPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPQTGKRYMHHYNFPPFSTGETGRLGSPRRRE
                     IGHGALAERALVPVIPSEDEFPYALRIISEVLESNGSSSMASVCGSTLALMDGGVPIK
                     APVAGVAMGLVKEGDDYVILSDIQGLEDHMGDMDFKVAGTADGITALQMDMKITGVSA
                     GLLQEALEQARAGRLEILGILREAIAEPRTEVSDFAPRVEVLKIPTDKIGMLIGPGGK
                     TINALQDQFGVNISVEDDGTVFVAGEGSVSRDAAGAIKGMMKDVEAGEIYNGKVVKTT
                     NFGAFVELTPGRDGLVHISRLAPGKQRVERVEDVVTEGDLIKVRVLEIDKQNRISLEK
                     LEG"
     gene            4953..6209
                     /locus_tag="AC16CM_13520"
     CDS             4953..6209
                     /locus_tag="AC16CM_13520"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3140898.1"
                     /translation="MADSNIRRKDLPGGLRVFTEPLAEATSVSLGVWIRAGSRDEKDE
                     VAGISHLMEHMLFKGTPDMDALGIAQAFEGIGAQENAATGEEYTVLYARFLPEHLGRA
                     LDVMSDMILRPTLVDLEREREVIVEEIKMYEDRPDQLADEYLSSLIFNEDPLGRPIIG
                     SAETVRGVDHTTLDGFHKDTYTGANVFVVAAGKLDPEDVERTVEEKLSGLPEGEPFVR
                     EASPTPPASRFYFRFKETEQYHVSMGSMGIPARSEDRYAMAALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDAGAIKTYVGSTTGNVEEAVQVIVEQLGKIMEEDVEGEE
                     LARTKQQLKSSTLLALESTSARMNRVGRSVITGAELLTPEEISARIEAVTADDISRLA
                     REHLRTDQMYLSAVGPKELDLGRYLN"
BASE COUNT         1235 a         1853 c         2124 g         1007 t
ORIGIN      
        1 cgccgatggt ctccgcgctg aaaagggaag gccagaggct ctacgacctg caccgccggg
       61 gcattacggt ggagcgcgaa ccccgtcccg taacagtcca tcgcctcgcc ctgacggcct
      121 tcgacccggc cgagaagacc gcagacttcg aggtctcctg ctccagcggt acctacgtga
      181 ggacgctcgt tgccgacgta gccgaccgcg cgggttctgg cgcctacctg accgccctgc
      241 gccgcaccgc cgtgggacac ctctccgtga aggactcctc cccacccggg gccctgagcc
      301 ctaccaccct acgtaaccgc ataatacctc cagcggaggt ggttagccac ctgtccgcgg
      361 tggtcgtttc gggtgaagtt cgcgcctcgg tgtgccatgg ggtacggatg gaggcatgcg
      421 ggatcgacgg cagctaccgg gtgatgtgtg gggaggagtt gctcgcggtg tacagggatg
      481 agggtaaaga gggaagggcc gaggtggtgc tttgcgcggg gtagtcgtcg cgcttggcaa
      541 cttcgacggg gtgcatctcg gccaccagga ggttctgagg cgggcggtcg aagaaggtcg
      601 gagagcggga aggcaggtcg ttgcggccac cttcaggcca catccgcggg ctgtgttggg
      661 ggctggggat ccgccaaggt tactgtcccc catggatgtg cgccgggagg cattggttcg
      721 ttatggggcc gatgaggtag tagagatacc attcgacctg gagctgtcca gaaagagccc
      781 ggagcaattt gtgcgtgacg tgctagttgg ggacatcggg gcggctgtgg tcgtagtcgg
      841 cgagaacttt cggttcgggt acagggccgc cggcgacttc ggggacctcg accggctaat
      901 gcgggagatg gggggggagg ccgtggcggt ggacgtacgg gcggcaggtg ggggacccga
      961 catcagctct acccggatca gggccctcat tgcgcaagga aaggtggagg aggcggccgg
     1021 gcttctaggt cgggccttcg tgctcagggg agaggtcgtg gtgggggaca ggcgcggaag
     1081 gaccataggg tttccaaccg ccaatgtgct tcccgatgag gaggccgttg tacccgccta
     1141 cggtgtatac gccgggttcg tgctcgtcgg cgaccaggag ttcgtcgcct gcaccaacgt
     1201 cggtgttgcg cccaccttcg cgcgcaccga tagccgcgtg gaagcctacc ttctggattt
     1261 cgacggcgat ctgtacgggc gcaccgtcga cgtgggcttt acgcacagga tacgtggcga
     1321 gaagaaattc ttgggcatag acgagttgaa ggagcagatc caaaacgatg tagagactgc
     1381 aaggtcgctg gcgaaaaacg ggagatagcc cagcccccgg accgtcaagg gaaccaaccc
     1441 tgcgcggcgc gtgtattagc ggtagacgta tcccacgcgc caggaggcga gatgtcccgc
     1501 acccggatcc gacaaggcag aaagtactgg acggtggcac tggtgctgct cgcgctcgcg
     1561 gtcccggcct gcgcaaacag cggggaagag actctgtcag atagcgacga ggtagccagg
     1621 gaccgggcgg acggggtctc gaggtcgcaa cccgaagcgg gggctggagc gccggagccc
     1681 gccagtacga cgcagggtgg cacggcacag ggcgtttcgg gggccgtggt gcccccggaa
     1741 gacttcgacc gcaagatcat caagtcggcg gagatcggtc tgacctcgaa ggacgttcgt
     1801 ggcgaggctg cggtggccca gcaggtcgtt gccggcgtcg ggggcactac cgtcagctcg
     1861 cagacctacc gctcgggtga caccctgtat gcgaatctca tcctctccgt cccctcagaa
     1921 cacttcgagc gggcgctgga tgagatcagg ggcctcgggg acgaggttac gatggatacg
     1981 gtctcgggcc aggatgtgac cggggagtac gtggacctgc aatcgcggga gcggaacctt
     2041 ctggcagcgg agcagtctct cctcgacctg tacaacaggg ccgccgatgt gcaggaagcg
     2101 ctctccatcc agagcgagct tacagaggta cggggtgaga tcgagcaggt ccagggcagg
     2161 atgcagtacc tcaaacagag cagcgacatg tctcggatct ccgtcaacat ccagcccgtg
     2221 agcaaccccc cgaaaccgcc gccggcctgg gacccggctc gcgaggtggc ccgggcctgg
     2281 aatgcttccc tggccgtctt gcagggtgtg gcgcttgcag tcatctccac cgttgtgttc
     2341 gggtggtgga tcatcccgcc cctcgccctg gcggcttggt ggctgaggcg aaggctccgg
     2401 catgagagcc tcaccccgcg ccccaccgag ccggaatgag cgcggcaaac gctagttcca
     2461 tccacgaacc tctaccttga ccgaccttga taccgaggtc cggacaggac ttccaacatc
     2521 gttacgtgtg gtagcataag gggtcgaatt ggtgaaataa caaagaagaa gataaagagg
     2581 agaaacgaga agttggctgt agctgaacag aaggcagaga taatacaaga aaaccgcacg
     2641 cacgagggtg acacggggtc gcccgaggtg caggttgcgg tgctcacccg acgcatagcg
     2701 cacctcacgg agcatctgcg tgagcacaag cacgattacc attcgcggcg cgggctgctc
     2761 aagatggttg gcaggcgccg ccggttgctc aagtacttgc agaacaagga cgtcgagcgc
     2821 tacagggcgc ttatcgccaa gctcgggcta cgccggtagt ccgggtacca ggaagaaacc
     2881 agaggagaac accatatgcg tctagagata ccagtaggcg agcgctcgat cgtgctcgaa
     2941 accgggaagt tggccaagca ggctggcgga gcggtcgttg ccaggctggg tgacaccacc
     3001 atactttcaa ccgccactcg ctcgtcgaac ccgcggcccg gcgccacgtt cttgcccttg
     3061 agcgtggaca tagaggagcg gatgtcggcg gcaggcaaga tccccggcgg gttcataaag
     3121 agggagggca ggccctccga gcgtgcgatc ctcacctccc gccttaccga caggccgctc
     3181 aggcccctct ttccgaagaa ctatcactac gagatacagg ttatcgggac cgtgctcgtc
     3241 gcagaccagg agaccccttt cgacaccgta agcatggtcg gggcgtcggc ggcgctggcc
     3301 ctttcggacc tgcctttcca ggggccaata ggggccgtca ggatcgggcg ctcgctagac
     3361 ggggagttcg tcctgaaccc gacctacgag cagatctccg agagtgacct cgacctcgtc
     3421 gtggccggca ccaaggaggc catcaccatg gtcgaggccg gggccaacga ggtgcccgag
     3481 tccgtaatgg ttgaggcact tcagctcgcc caggagatcg tccagaacca ggcgctggcc
     3541 atagaggaat gggccggcga gtacggcaaa gagaaacagc agatagatgg gcccgaggag
     3601 aacactttcg tcacctccgc gcgggagcag tacttcgacc gtgtcaaaga gggcatcgtc
     3661 cagaccgacc ggagggtacg tagcaccatg ctggccgaga tccgcgagga gatggtcgag
     3721 ggtcgcgagg aagaagagat accagcgatc accgccgccc tttccacggt ggagaaagag
     3781 gccttccgcg agctttacct gaaggaccac aagcgcacgg acctgcgtga gatggacgag
     3841 atccgtccga cctctgccga ggtggcggtg atcccgcggg tacacggcac ggggctcttc
     3901 acgcgtggcg agacgcaggt gctaagctcg ctcgcgctcg ctgacctcgg cctcgcgcag
     3961 cggctcgaca ccatcgagcc ccagaccggc aagcgctaca tgcaccacta caacttcccg
     4021 cccttctcca cgggcgagac cggccgcctc ggtagcccgc ggcggcgtga gatcggccac
     4081 ggggcgctcg ctgagcgcgc gctcgtgccg gtaatacctt ccgaagacga gttcccgtac
     4141 gctctacgca taatatccga ggtgctcgag tcgaacggtt cctcctctat ggcgagcgtg
     4201 tgcgggtcga cgctggccct catggacggc ggcgtgccga tcaaggcgcc tgtggccggc
     4261 gtggcaatgg gcctcgtcaa ggagggtgat gattacgtca tcctctcgga catccagggc
     4321 ctcgaggacc acatgggcga catggatttc aaggtcgccg gtacggccga tggcataacc
     4381 gcacttcaga tggacatgaa gatcaccggc gtctccgccg gcctgttgca ggaagccctc
     4441 gagcaggcca gggctggtcg tctggagatt ctgggcatcc tgcgcgaggc tatcgccgag
     4501 ccgcggaccg aggtctccga tttcgccccg agggtcgagg tgctcaagat cccgaccgac
     4561 aagatcggga tgctcatagg accaggaggg aagaccataa acgcccttca ggaccagttc
     4621 ggcgtgaaca tctccgtgga ggacgatggc acggtgttcg tggcaggaga gggcagcgtc
     4681 tcgagggatg ccgccggcgc tatcaagggc atgatgaagg acgtcgaggc cggggagatc
     4741 tacaacggca aggtggtgaa gacaaccaac ttcggcgcct tcgtcgagct cacgcccggc
     4801 cgtgacggcc tggttcacat ctcgcgcctc gcccccggca agcagcgcgt ggagcgggtc
     4861 gaggacgtgg tgaccgaggg cgacctgatc aaggtgcgcg tcctggagat agacaagcag
     4921 aaccgcatca gcctggaaaa actggagggc tagtggccga ttcgaacata cggcggaaag
     4981 acctgcccgg gggcttgagg gtctttaccg agcccctggc cgaggccacg agcgtctcgc
     5041 tgggggtctg gatcagggct ggatcccgcg acgagaaaga cgaggtcgcg ggcatcagtc
     5101 acctgatgga gcatatgctc ttcaagggca cccctgatat ggacgccctc ggcatcgccc
     5161 aggccttcga gggaataggc gcccaggaga acgcggcgac gggcgaggag tacaccgtgc
     5221 tctacgccag gttcctgccc gagcatctgg gccgggccct cgacgtcatg agcgacatga
     5281 tcctcaggcc gacgctcgtg gacctggagc gtgagcgtga ggtgatcgtc gaggagatca
     5341 agatgtacga ggacaggccg gaccagctgg ccgacgagta cctctcctcg ctgatcttca
     5401 acgaggaccc actggggcgt ccgatcatcg gctcggctga aaccgtgcgg ggcgtggacc
     5461 acaccaccct ggacggtttc cacaaggaca cctacacggg ggccaacgtc ttcgtcgtcg
     5521 ccgccggcaa gctggatccc gaggacgtag agcggacggt agaagagaaa ttgtccgggc
     5581 tgcccgaggg agagccgttc gtgagggagg caagcccgac gccgcccgct agccggtttt
     5641 atttcaggtt caaggagacc gagcaatacc acgtctccat gggctctatg ggcatcccgg
     5701 ccaggagcga ggaccgctat gccatggccg ccctcaacaa cgtgcttggc ggaggcatga
     5761 gcagccgcct ctttcaggag gtgcgtgaga agcgtgggct tgcctacgcc gtctactcct
     5821 accaccaggg ctactctgac gcaggggcga tcaagaccta cgtcggctcc accaccggca
     5881 acgtggagga agccgtgcag gtgatagtcg agcagctcgg caagattatg gaggaggacg
     5941 tcgaaggcga agagctcgcg cgcaccaagc agcagctgaa gagctcgacg ctgctcgcgc
     6001 tggagagcac ctcggcccgg atgaaccgcg ttggacgcag cgtcataacc ggcgccgagc
     6061 tattgacccc ggaggagatc tcggcccgca tagaggccgt gaccgcggat gatatctcgc
     6121 gcctcgcccg ggagcatctc agaacagacc agatgtacct ctccgccgtc ggaccgaagg
     6181 agctcgacct gggcaggtac ctcaattagg gctgtcagc
//
LOCUS       DBNGVD010000501         5422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3768382, whole genome shotgun sequence.
ACCESSION   DBNGVD010000501 DBNGVD010000000
VERSION     DBNGVD010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3768382"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /locus_tag="AC16CM_13525"
     CDS             complement(<1..2328)
                     /locus_tag="AC16CM_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3140899.1"
                     /translation="MSHGIRKVAVLGAGTMGAAIAAHCANAGLEVDLLDIAADDDDND
                     AVVEAGFERMKNARPAALMVEEVAGRIRTGNFQNHFDRVGEADWVLEAIIEKLEPKRE
                     LMRRLEETAKDDAIISTNTSGIPLHEISEERSESFKRRFVGTHFFNPPRYLKLMEIIP
                     TEDTDPEIVEAVRNFGERVLGKGGVIAKDTPNFIGNRIGTFAGMQSARYAFDNGYGIE
                     EIDAITGPLIGHPKTATFRLNDQVGLDIAVGVAENLYEAVPEDESREELKPPEKLQEM
                     LQKDLLGNKSGAGFYKRDKREGKTVFDVLDLETFEHHPAENPEIPIAEEAQKQGDLAA
                     RLKFLVDRADEDRHARFIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFAHQTGPF
                     RTWDLLGVRETVENMKAQDIAVGGWVEDMLEAGNESFYKTEDGTELQFSPVEREYVPV
                     REDPMYVSLDRLRDEGKELDRNDSASLLDLGDGVLCLEFHSKGNSIDADIVEMGNRAL
                     AALDRDDVVGLVIGNEGRNFSVGANLGEMAHAVQNGNLEAVGESVDALQNLLMAFRFA
                     PKPIVAAPHGQTLGGGLEVCLHSDRIVAAGETYMGLVEVGVGLITAGGGTKEMARRLV
                     SPPLRIAPDTPALPFLQKAFKQIALAKTATSAIEAQDMGFLTDNDRIVMNADHLISAA
                     KREVLDLADGYSPPERGANVYAAARSARAALELGVKTMQWGHYASEYDGVIAGQVARV
                     LTGGGLSLPQWVPEEYLLKLEKEAFLDLLQQEKTHE"
     gene            complement(2343..2711)
                     /locus_tag="AC16CM_13530"
     CDS             complement(2343..2711)
                     /locus_tag="AC16CM_13530"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3140900.1"
                     /translation="MERATPKDIFLSEVKTRDVFPLLVLHIVREEPAYGNSIMTRIRE
                     ISAGAMSVSPNTIYPLLRRLEEKGYVEGEWERPDTRSRRFYKITPAGETKYAEIKDRF
                     EGHLLRVREAIEALRREIYG"
     gene            2864..4063
                     /locus_tag="AC16CM_13535"
     CDS             2864..4063
                     /locus_tag="AC16CM_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3140901.1"
                     /translation="MIADVTNSLGTDYYLLDELYTDEEREIRDRVRAFADAEVIPIIS
                     GYWERAEFPFELVPKVAELNIAGATIEEYGCPGMSQVAAGLCAIEMARGDGSLNTFFG
                     VHSGLAMGTVAICGSEEQKQRWLPDMARMEKIGAWGLTEPDHGSDSVMLETTARRDGD
                     EWVINGKKRWIGNATFADLVIIWARDVEDGQVKAFVLERDENGDHHPGYSTELITGKM
                     GKRAVWQPDIYLEDVRVPDENRLRGANSFKDTNKVLTATRSGVAWETIGHAIAAYEAA
                     LQYSQERVQFGKPIASFQLIQNKLSGMLAEITNMQLMCFRLAQLQEQGKMTGPMASLA
                     KMNNARKAKQVCSDARDIMGGNGVLLEYHVARHLSDMEIVYTYEGTDTIQSLIVGRDI
                     TGISAFV"
     gene            4229..5245
                     /locus_tag="AC16CM_13540"
     CDS             4229..5245
                     /locus_tag="AC16CM_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3140902.1"
                     /translation="MKAVQLHTLEGPDELVYEEVPDPEPGPGETVVRLRAAALNRRDL
                     FATRGQYPGVTPDILPGIPGSDGSGEVVARGDGADGPGEGTEVVVNPALYWGDNPRVP
                     GKEYRILGLPDDGTFAQFIKVPSDHVFPKPSHLSHEEAAALPLAALTAYRALVTRGGV
                     TEGETVLVPGVGGGVATFVVQIASSMGARVFVTSGSDEKIEEAKELGAEGGVNYNSEN
                     WSRELKSMSGSVDLSVDHVGGDAFNSLVSLARPGGRIVVFGATAGPTPKMMTIRVALK
                     HLDVLGTAMGTPEEFGAMLDFYAEHGLRPKVNETFSLEDIAQSLHLMEEGSGMGKIVL
                     SIPA"
BASE COUNT          983 a         1675 c         1717 g         1047 t
ORIGIN      
        1 ttcgtgggtc ttttcttgtt gcaggagatc caggaaggct tctttttcca gcttgaggag
       61 gtattcctct ggcacccact gggggagcga gagaccccct ccggtgagga cccgggctac
      121 ttggccggct attacaccgt cgtattcgct tgcgtagtgg ccccactgca tggtctttac
      181 gccaagttcc agggcggccc gggcggagcg ggccgcggca tagacattcg ccccacgctc
      241 gggtgggctg tagccgtcgg cgaggtcgag aacctcgcgt ttggcggcgg agataaggtg
      301 gtcggcgttc atcacgatgc ggtcgttgtc ggtgaggaaa cccatgtcct gcgcttctat
      361 ggcgctggtt gcggttttgg cgagggctat ctgtttgaag gccttctgga ggaagggcag
      421 agccggggtg tcgggagcga tgcggagcgg cggggagacg aggcgccggg ccatctcctt
      481 ggtgcctcct ccggcggtga tcaggccgac accgacctcg acgaggccca tgtaggtctc
      541 gcccgccgcg acgatcctgt cggagtggag gcagacctcg aggccaccgc cgagcgtctg
      601 accatgcggg gccgctacga tgggtttggg agcgaagcgg aaggccatga ggaggttctg
      661 gagcgcatcg acgctctcgc ctacggcctc caggttcccg ttctggacgg catgcgccat
      721 ctcgccgagg ttggcaccta cggagaagtt gcgcccctcg tttccaatga ccaggccgac
      781 cacgtcgtcg cgatcgagcg ccgcgagcgc cctgttgccc atctcgacga tgtcggcgtc
      841 tatggagtta cccttggagt ggaactccag gcagagaacg ccgtccccga ggtcgagcag
      901 gctggccgag tcgttccggt caagctcttt gccctcgtcc cgcagccggt cgagggagac
      961 gtacatcggg tcctcgcgga cgggtacgta ctcccgttcg accgggctga actgcagctc
     1021 ggttccgtcc tccgtcttgt agaagctctc gttgccggct tcgagcatat cctcgaccca
     1081 gccgccgacc gcgatgtcct gtgccttcat gttctcgaca gtctcgcgca cacccagcag
     1141 atcccaggtc ctgaacggtc cggtctggtg ggcgaagccc cactccatgg cgtgatccac
     1201 gtcttctagg gtgtcggaga tctccggcac ccgccgcgac gcgtaagcca tgtacggcag
     1261 caacgtgtcc cttatgaagc gggcgtgcct gtcttcatcc gctctgtcta ccaggaactt
     1321 gagcctggcg gcgaggtcgc cctgcttctg cgcctcctcg gcgattggga tctcggggtt
     1381 ctcggcagga tggtgctcga aggtttcgag gtcgagcacg tcgaagacgg tcttgccctc
     1441 acgcttgtcc cgcttgtaga agcccgcgcc gctcttgttg ccgagcaggt ccttctgcag
     1501 catctcctgc agcttctcgg gcggcttcag ttcctcgcgc gactcatcct cggggacggc
     1561 ctcgtagagg ttctcggcga cgcccacggc gatgtccagg ccgacctggt cgttgaggcg
     1621 aaacgtggcg gtcttgggat gcccgatcag gggtcccgtt atggcgtcta tctcctcgat
     1681 gccgtagccg ttgtcgaagg cgtagcgggc tgactgcatg ccggcgaaag tcccgatcct
     1741 gttcccgatg aagttggggg tgtccttggc gatgacgccg cccttgccga gcacccgctc
     1801 cccgaagttc cttacggcct cgacgatctc ggggtccgta tcctccgtgg gtatgatctc
     1861 cataagcttc aaataccgcg gcgggttgaa gaagtgggtg ccgacgaaac gccgcttgaa
     1921 actctcggac cgctcctcgg agatctcgtg cagcggtatg cccgaggtgt tggtcgagat
     1981 gatggcgtca tcttttgccg tctcctcgag gcgccgcatc aactcgcgct ttggctcgag
     2041 cttctcgatg attgcctcga gcacccagtc ggcctcgccg acgcggtcga agtggttctg
     2101 gaagttgccg gtcctgatcc tgcccgcaac ctcctcgacc atgagcgccg ccggcctggc
     2161 gttcttcatc ctctcgaagc cagcctcaac aacggcatcg ttgtcgtcgt catcggcggc
     2221 tatatcgagc aagtccacct ccagccccgc gttggcgcag tgggcggcga tggccgcgcc
     2281 catcgtgcca gcccccagta ctgcgacctt cctgatcccg tgcgacatag atacccccac
     2341 tcctatccgt aaatctcccg acgcagggcc tcgatagcct ctcttacccg caacaggtgg
     2401 ccctcgaatc tgtccttgat ctccgcgtac ttcgtctccc cggcaggcgt tatcttgtag
     2461 aagcgccggc tcctcgtgtc aggcctctcc cactctccct ccacgtaccc cttctcctcc
     2521 agccgccgga gcaacggata gatggtgttg ggagagacgc tcatcgcccc tgcgcttatc
     2581 tcccgtatcc tcgtcatgat gctgttgccg taagcaggct cctccctgac gatgtgcaag
     2641 acgagcaacg gaaatacgtc ccgcgtcttg acctcgctca gaaaaatgtc cttcggcgta
     2701 gccctctcca ctctctcccc taccggctca gccaacgacc ctcgacctgc cccttcatcg
     2761 aaaaacttgt caaaattgat aataacacgg ataataagtt cacgtaacca tccaggcccc
     2821 tgtagagttg gggtttaggg aggtccgaga gaaggaggag cgagtgatcg ccgacgtcac
     2881 gaattcgtta ggtaccgact actacctgct cgacgagttg tacacggatg aggagcggga
     2941 gatccgggac cgggttaggg ccttcgccga cgccgaggtc atacccatca taagcggcta
     3001 ctgggagagg gccgagtttc cgttcgagct cgtgccgaag gttgccgagt tgaacatcgc
     3061 cggcgccaca atcgaggagt acggctgtcc cggcatgagc caggtcgcgg cgggtttgtg
     3121 tgccatcgag atggcgcggg gggacgggag cctgaacacg ttcttcgggg tccattccgg
     3181 gcttgccatg gggaccgtgg ccatctgcgg ctccgaggag cagaagcagc ggtggctacc
     3241 cgacatggcg aggatggaga agatcggggc ctggggcctg acggagccgg accacggatc
     3301 tgattcggtc atgctggaga ccacggcccg gcgagacggg gacgagtggg tcataaacgg
     3361 caagaagcgt tggatcggca acgccacctt cgccgacctc gtcatcatct gggcccgcga
     3421 tgtggaagac ggccaggtca aagccttcgt tctcgagaga gacgagaacg gcgaccacca
     3481 tcccggttac tcgacagagc tcataacggg caagatgggc aagcgggccg tctggcagcc
     3541 ggatatctat ctagaggacg tgcgagttcc agacgagaac cgtctgaggg gcgccaactc
     3601 cttcaaggac acgaacaagg tacttaccgc cacccgtagc ggcgtggcct gggagaccat
     3661 agggcatgcc atagccgcct acgaggcggc gctgcaatac tcgcaggagc gggtgcagtt
     3721 cggcaagcca atcgccagct tccagctcat ccagaacaag ctctccggca tgctggccga
     3781 gatcaccaac atgcagctca tgtgcttccg ccttgcccag ctgcaggagc agggcaagat
     3841 gaccggtccc atggcctcgc tcgccaagat gaacaacgcc cgcaaggcca agcaggtctg
     3901 ctccgacgcc cgagacatca tgggcggcaa cggtgtactc ctcgagtacc acgtcgcacg
     3961 gcacctctcc gacatggaga tcgtgtacac ctatgagggg acggatacca ttcagtcgct
     4021 catagtgggg cgggacatca cggggataag cgcgttcgtg tagctatcag cttttagctt
     4081 tcagcggtca gccagagatt cgatgggcgc cacctgggta caaataaggc gtcatacggt
     4141 ggtccgtgct ttaggtacac gaaagttacg gcaagaacca agaagctgac cgctgaaagc
     4201 taaaagcgct cgaccgaagg gagagcaaat gaaagccgtt caattgcaca cgctggaagg
     4261 tcccgacgag ctggtctacg aggaggtgcc agatccggag cccggtccgg gtgagaccgt
     4321 tgtccggctg cgggcggcgg ctttgaacag gcgggatctc ttcgcgacca ggggacagta
     4381 ccctggtgta acgccggata tcctgccggg catacccggc tctgacgggt cgggggaggt
     4441 agtcgccagg ggcgatggcg cagacggtcc cggcgaaggt accgaggtcg tcgtgaaccc
     4501 ggcgctttac tggggagaca acccgcgggt gccgggcaag gagtacagga tcctcggtct
     4561 tcccgacgat ggcacgttcg cccagttcat caaggtgccc tccgatcacg tctttcccaa
     4621 accttcccac ctgtcgcacg aggaggccgc ggcgttgccg ctcgccgcgc taacggccta
     4681 cagggcgctc gttacacgtg gtggggttac cgaaggtgag accgtgctcg tgccaggcgt
     4741 cgggggcggg gtggccacgt ttgtggttca gatcgccagc tcgatgggcg cgcgcgtctt
     4801 cgtcacctct ggtagcgacg agaagatcga agaggcgaag gaactgggcg cggagggtgg
     4861 cgtcaactac aactcggaga actggtccag agagctcaag agcatgtcgg ggagcgtaga
     4921 cctttcggtc gatcacgtcg gcggcgacgc cttcaactcc ctagtctcgc tcgccagacc
     4981 gggaggccgc atcgtcgtgt tcggcgcgac cgccggcccg acgccgaaga tgatgaccat
     5041 ccgcgtggcc ctcaagcatc tcgacgtcct cgggaccgcc atgggcacgc cggaagagtt
     5101 cggggctatg ctcgacttct acgccgagca tggattacgc ccgaaggtaa acgaaacctt
     5161 ttcgctagag gacatcgccc aatctctcca tctcatggaa gagggtagcg gtatgggcaa
     5221 gatcgtgctg agcatcccag cctagcttgc cagaccgaat gccccgcgag ggcccgtgat
     5281 taccagagct gtctgaaccg gctcacggct gctctagacg tgggcggcgc gttctcagaa
     5341 ggtgaggcgc tccagtcggg ctcacgcacg agttgtgtca ccaacgggcc gaacatatca
     5401 tgacgcagtt cggtcaggcc gg
//
LOCUS       DBNGVD010000502         3162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2857353, whole genome shotgun sequence.
ACCESSION   DBNGVD010000502 DBNGVD010000000
VERSION     DBNGVD010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2857353"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /gene="rhaD"
                     /locus_tag="AC16CM_13545"
     CDS             <1..1552
                     /gene="rhaD"
                     /locus_tag="AC16CM_13545"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3140903.1"
                     /translation="QVGEAVKNNPNARFVLLAKHGLVTWGDTHEESYGRTIEAINRAA
                     EFVAGRADEPFGGRMVEPLEQDRREELLADVLPALRGALSSGADESSHKILRTDFTSE
                     DVLTFVCGRDSEEVSQVGAACPDHLVRTKVRPLWIDFDPEKEGAEELGAKLREGVARY
                     REDYEAYFSRHEEADEEMFDPNPRVVYIVGVGLVAAAKNAKEANLSRDFAYRAINVMR
                     GAHALGGYVSLTEEESYAVEYWPMELYKLTLAPPPDELAGRVALVTGGAGGIGSAVAR
                     SLASKGACVVVCDLDEKGAAEVAEELAEPGIPARADVTDEDEVDLAYRRAVLEYGGVD
                     VVVSNAGLASSAPIEETSVEMWDKNHAVLARGYFLVARGAFRIMKEQGIGGSIVFVAS
                     KNAMAAGKNAAAYSSAKAAELHLARCLAEEGGAERIRVNTVNPDAVLQGSRIWDSGWR
                     ETRAAAYGIEPDELEDYYRNRNVLKISVLPENIADAVLHFASEARSSRSTGNVLNVDG
                     GVKDAYSR"
     gene            1667..1981
                     /locus_tag="AC16CM_13550"
     CDS             1667..1981
                     /locus_tag="AC16CM_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HIR3140904.1"
                     /translation="MAQYAWVLEVRPGYEEEYVRRHQEIWPEMVESLRAAGISNYSIF
                     RHGLTLFGHFETDDIDSTREYLANDETNRRWSEWMDPIMKVDIDPTTDFPYLLPLQWH
                     MD"
     gene            2107..2295
                     /locus_tag="AC16CM_13555"
     CDS             2107..2295
                     /locus_tag="AC16CM_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140905.1"
                     /translation="MELIELGGIFVLMTLAAFAVNAVASVALRDLMLAAPAARRWIER
                     TFGAILIGFAAKLAFTDR"
     gene            2415..2789
                     /locus_tag="AC16CM_13560"
     CDS             2415..2789
                     /locus_tag="AC16CM_13560"
                     /inference="COORDINATES: protein motif:HMM:NF036395.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase domain-containing protein"
                     /protein_id="HIR3140906.1"
                     /translation="MIDEPLAQIEREVLGWDGVFSKRDEHGPGGIGVTGYRYGDRETG
                     GPQIGHVHDDGHADFRFPREVRDELIRTGRAIPHPAFPDSRTTVSYQIRNPEDVEGAL
                     DLFRMNYERRKEQAARKVAPRS"
     gene            2837..>3162
                     /locus_tag="AC16CM_13565"
     CDS             2837..>3162
                     /locus_tag="AC16CM_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3140907.1"
                     /translation="MDTVVPGLYASEPEPLGFGPSLEIRAYLLHREPGNLLVYRSEAL
                     EQDVKTVGDLGGISRQYLNHFHEASPACDRVAGTFGAPLYVHEEDAAATAEVCNVDAT
                     FSERHKL"
BASE COUNT          623 a          980 c         1056 g          503 t
ORIGIN      
        1 acaggtcggc gaggccgtaa agaacaatcc gaacgcgagg ttcgtcctcc tggccaagca
       61 cggcctcgta acctggggcg acacccacga ggagagctac gggcgtacca tcgaagctat
      121 caaccgcgcc gccgagttcg tcgccggccg ggcagacgag cctttcggcg gtagaatggt
      181 cgaacctctg gagcaggacc gcagggaaga gctgctcgcg gacgtcctgc ccgccctgcg
      241 gggggcactc tcctccggag ccgacgagtc ctcccacaag atactaagga ccgatttcac
      301 ctcagaagac gtacttacct tcgtctgcgg acgcgactcg gaggaggtct cccaggtcgg
      361 cgccgcctgc ccggaccacc tggtcaggac caaggtcagg cccctgtgga tagacttcga
      421 ccccgaaaaa gagggcgccg aagagctcgg ggcgaagctt cgtgagggcg tcgcccgcta
      481 cagggaggac tacgaagcgt acttctcgcg ccacgaggag gcggacgagg agatgttcga
      541 cccgaaccca agggtcgtct atatagtcgg cgtcgggctc gtcgccgccg cgaagaacgc
      601 caaggaggcc aacctctcca gggacttcgc ctacagggcc ataaacgtga tgcgcggcgc
      661 ccacgccctt gggggttacg tctcactgac ggaagaagaa tcctacgcgg tcgagtactg
      721 gccgatggag ctctacaagc tgaccctcgc cccgcccccc gatgagctcg ccggcagggt
      781 cgccctggtt acgggcggtg ccggtggtat aggcagcgcc gttgccaggt ccctcgcctc
      841 gaaaggggcc tgcgtcgtgg tctgcgacct cgacgagaaa ggtgctgcgg aggtagctga
      901 agaacttgcc gagcctggca tcccggcgag ggccgatgtg acggatgagg acgaagtgga
      961 cctcgcctac cgtcgcgccg tgctggagta tggcggcgtc gacgtggtcg tgtccaacgc
     1021 cggcctcgct tccagcgcgc ccatcgagga gacgagcgtc gagatgtggg acaagaacca
     1081 cgccgtcctt gccaggggct actttctcgt cgcgcgcggg gcttttcgaa tcatgaaaga
     1141 gcagggcatc ggcgggagca tcgtgttcgt cgcctccaag aacgctatgg ccgcgggcaa
     1201 gaacgcggcg gcgtactcgt cggccaaggc cgccgagctg cacttggcga ggtgcctggc
     1261 ggaggaaggc ggagcggagc gcatcagggt gaacaccgtc aaccccgacg cggtgttgca
     1321 ggggtcaagg atctgggact ccggctggcg cgagacgcgc gccgccgcct acggtataga
     1381 gcccgacgag ctcgaggatt actaccgcaa ccgcaacgtg ctcaagatca gcgtgctgcc
     1441 ggagaacata gccgatgccg tgctccattt cgcctccgag gcgcgttcgt cgaggagcac
     1501 aggcaacgta ctcaacgtcg acggtggcgt taaagacgcg tattccaggt aggtttcagg
     1561 ctacaggcgt caggtctcag gtgaggttgg agggaccggg cgggttgcgg gaggctcggc
     1621 ttccgctgcg ctgcccggtc cgggttaccg ttgcaggggg atagggatgg cacagtatgc
     1681 gtgggttctt gaggtcaggc cggggtacga ggaggagtac gtgcgccggc accaggagat
     1741 ctggcccgag atggtcgagt cgctcagggc ggccggcatt agcaactact ccatcttccg
     1801 ccacggcctg accctcttcg gccacttcga gaccgacgac atcgacagta ccagagaata
     1861 cctggccaac gacgagacca acaggcgctg gagcgagtgg atggacccca tcatgaaggt
     1921 tgatatagat cccaccaccg actttcccta cctcctcccc ctgcaatggc acatggactg
     1981 agggcccgca cccaggacaa cgcttccgag gctgtgaacg cctgatcctt ccactacgtc
     2041 gttcccgcga aagcaggaat tcacgagatc gaactcgaga ctagtcggaa agacaggctg
     2101 ctcgacgtgg agctgataga gctcggcggg atcttcgtgc tcatgaccct cgccgcgttc
     2161 gccgtcaacg ccgtggcgag cgtcgctctc cgcgacctca tgctcgccgc gcctgcagcc
     2221 cgacggtgga tcgaacgtac gttcggtgcc atcctgatcg gtttcgccgc caagctcgcg
     2281 ttcacggacc ggtgacgtag accgaacctg tacgaaggtg caggcagaag accgttctcc
     2341 cgcccgatgt gtagtccctc tctacagcgc aagatatgta gtgcgcaagg ctggctatga
     2401 gagggagaag caggatgatc gacgaaccgc tggcgcagat agagcgcgag gtactggggt
     2461 gggatggggt cttctcgaag agggacgagc acggtcccgg cgggatcggt gtaaccggct
     2521 acaggtacgg tgaccgtgag acgggggggc cccagatcgg gcacgtccac gacgacggcc
     2581 acgccgattt caggtttcca agagaggtga gggacgagtt gatccgtacc gggcgggcca
     2641 tcccccaccc cgcgttcccc gacagccgga cgacggtgag ctaccagatc cggaaccccg
     2701 aggacgtgga gggggccctg gatctcttcc gcatgaacta cgagcgaagg aaggaacagg
     2761 ccgcacgcaa ggtcgcaccc cgaagctaac ggaggcacgg ccgaaacggg gtcgccagag
     2821 gaagagaggt aatctcatgg acaccgtggt acccggcctg tacgcttcgg agcccgaacc
     2881 gctcgggttc ggaccctcgc tcgagattcg tgcctacctg ctacaccgcg agccaggcaa
     2941 cctgctcgtc tacaggtccg aggcgctgga gcaggacgta aagacggtgg gcgacctggg
     3001 cggcatctcg cgccagtatc tcaaccattt ccacgaggca tcgcccgcct gtgaccgggt
     3061 cgccggcacg ttcggcgcac cgctttacgt tcacgaggaa gacgcggcgg ccaccgccga
     3121 ggtctgcaac gtggacgcga ccttctcgga gcgtcacaag ct
//
LOCUS       DBNGVD010000503         6907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_308950, whole genome shotgun sequence.
ACCESSION   DBNGVD010000503 DBNGVD010000000
VERSION     DBNGVD010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_308950"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16CM_13570"
     CDS             complement(<1..356)
                     /locus_tag="AC16CM_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3140908.1"
                     /translation="MSERFYVEGNRRLEGEVEVSGAKNAALKFVAAALLAPGKTTLHH
                     VPRIKDIYTMLALLDELGVETSFSGSTLEIDASTVHSYTAPYDLVRQMRASLVVLGPL
                     VARFGEAEVSAPGGCNL"
     gene            complement(527..982)
                     /gene="atpC"
                     /locus_tag="AC16CM_13575"
     CDS             complement(527..982)
                     /gene="atpC"
                     /locus_tag="AC16CM_13575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR3140909.1"
                     /translation="MAEDNGQRREGRQLICRVITPEEVIYDGEANLVVARIADGDLGV
                     LVDHSPLVSTVEVGEVRIKEGDDQLVYATSDGFFKVSENLVQVLVEEAVSVEEIDVDA
                     AESRVEEAERELSEVSEDDEDRDRVTAEIERRQRMGENLVRLARKYGGS"
     gene            complement(982..2652)
                     /gene="atpD"
                     /locus_tag="AC16CM_13580"
     CDS             complement(982..2652)
                     /gene="atpD"
                     /locus_tag="AC16CM_13580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3140910.1"
                     /translation="MSESESGQSFNRTTFGGERGEVDNGHAAQQENDGRDGREERKSG
                     VGTVVEVKGPVVDVRFSPEDIPEIFNAVTVDLEVNEETTELTLEVQQLLGDDMVRTVA
                     MASTDGLRRGVDATDTGRPIAVPVGKNVLGRVLDVLGNPVDEAGPVEDPEDYWPIHRP
                     APRFEDLTTQSEQFVTGIKVIDLLCPVLRGGKVGLFGGAGVGKTVVIQELINNIALQY
                     EGTSVFAGVGERTREGNDLYGEFQEAGILENVGLVFGQMNEPPGARFRVGLTGVTLAE
                     YFREELGQDVLFFVDNIFRFVQSGNEVSALMGRMPSEVGYQPTLGTEMGALQERITST
                     KKGSITSFQAVYVPADDFTDPAPFTVFAHLDSTIELSRSLAAQGIYPAVDPLSSSSTI
                     LDPAVVGDEHYAVAQEVKNVLQRYKELQDIIAILGIDELSEEDKVIVGRARRLQRFLS
                     QPFFVAEQFTGMEGKFVELDETIRSFKEVVEGKHDDLPEQAFYLVGTIDEAVEKARGL
                     SDEDEVESSEESDEETSEDSGRAEASTEDSDEDSGEQESGGNDENDGR"
     gene            complement(2668..3528)
                     /gene="atpG"
                     /locus_tag="AC16CM_13585"
     CDS             complement(2668..3528)
                     /gene="atpG"
                     /locus_tag="AC16CM_13585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3140911.1"
                     /translation="MATARDLQRQINTVKNQSRVFSALQTVSSVKFRKAEARVKKARP
                     YAENVEEMMRDVAGSASGDLPLLVGREVNRVAICTLTADRGLAGGFNAQVLRRTVGLR
                     NEKDQNALQVASGRKAVAFFRFRRVELAEVFTGFTDNPAYEKAREIGSALTGLFEEEE
                     ADEVYLVYNRFDSALTQTPVVKRLLPVVEEGDEEQDDSASYMEYVPDAETLLKRLVPR
                     YVETMVWQALLEGAAGEHGARMTAMKNATDNANDLVRDLTLQMNKARQAQITQEILEI
                     VGGAEALSAG"
     gene            complement(3532..5109)
                     /gene="atpA"
                     /locus_tag="AC16CM_13590"
     CDS             complement(3532..5109)
                     /gene="atpA"
                     /locus_tag="AC16CM_13590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3140912.1"
                     /translation="MGRLRPEEISSILKGAITDYENRVRTEEVGQVLEVGDGIARVHG
                     LTNAMYQEMLEFEDSRGEKITGLALNLEEDNVGVIIAGDDTHIQEGNTVTRTERLASV
                     PVGEGVLGRVIDALGRPMDGKGEIEAEETLPVEIVAPGIIRRQPVKQPLQTGIKAIDA
                     MIPIGRGQRELVIGDRKTGKTSILLDTIINQADQNVKCVYVAIGQKDSTVAGVVAALE
                     EAGAMEYTTVVNASAAQSATLQYLAPYSGCAIGEFFMNRGEDSLLIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDAAGGGSLTALPVIETKAGDISAY
                     IPTNVISITDGQIFLDSDLFNSGQRPAIDTGNSVSRVGSSAQIKAMKDVAGTLRLDLN
                     QYRELASFAQFGSDLDRATQQTLNRGERLTAILRQGELDPMPVEEQVVVLYAASNGYL
                     DEVEPDVVMDWERQFRDFMRDSHGEVLDSIRDEQKLSDENGQKLDEAIENFNDNFEPG
                     QTSLVEVSPGDDEEEEG"
     gene            complement(5109..5648)
                     /gene="atpH"
                     /locus_tag="AC16CM_13595"
     CDS             complement(5109..5648)
                     /gene="atpH"
                     /locus_tag="AC16CM_13595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR3140913.1"
                     /translation="MSAVSTYAEALFGAARERDELEEVLSDLGEFVEALHDSEELRLF
                     FYGGQVPERQKRRALDGLTEGMKTSTTNFLKVLVDNGREEVLKEVLARYEELVKQHLG
                     RIEVEVTTAVELSEDQRDRLRDRLRGVLEGQEIILETNVDPGLVGGAVFRYAGRMMDG
                     SIRGRLESLREGMLERSVV"
     gene            complement(5645..6196)
                     /gene="atpF"
                     /locus_tag="AC16CM_13600"
     CDS             complement(5645..6196)
                     /gene="atpF"
                     /locus_tag="AC16CM_13600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3140914.1"
                     /translation="MAMVLAQLDPTGIFDLGNTSLIFWEVITFFILLFLLVRYVYPPI
                     RKQIQDRQAQIEQSIDEAEKTRAEARELLAEYRRQIEEARGEGRRILDEARKQAEAQR
                     ERTKREAREEGDRIIQRAREEIDRERESAVREVRSEVADMVVQASEQVIGRSIDRDEH
                     ERLISQALDDLESEVAGSRSGTA"
     gene            complement(6219..6491)
                     /gene="atpE"
                     /locus_tag="AC16CM_13605"
     CDS             complement(6219..6491)
                     /gene="atpE"
                     /locus_tag="AC16CM_13605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3140915.1"
                     /translation="MDVLALVVPMLALLQASGDVTMIGVGIAAGTAVIGPGAGMGYLI
                     GQTIASIHRQPEAFDQTRTLMFLGIGLVEAFALYGIVFALLIAFVL"
     gene            complement(6545..>6907)
                     /locus_tag="AC16CM_13610"
     CDS             complement(6545..>6907)
                     /locus_tag="AC16CM_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3140916.1"
                     /translation="LTQYEGFRRNGAGAYLKSFAPPGLPLKPVMVPFMWSIEVLGEFT
                     KPLTLGMRLYANILAGHLIIYVFLGLILYFGTIVAVVSVPLSVVFYGFEIFVAVLQAY
                     IFAILTQVYIELAMFRDH"
BASE COUNT         1103 a         2326 c         2122 g         1356 t
ORIGIN      
        1 aggttacagc cgcctggggc ggagacctcg gcctcgccga aacgcgccac aaggggtccg
       61 agcacgacga ggctggccct catctggcgc accagatcgt agggggccgt gtaggagtga
      121 acggtggaag cgtctatctc cagggtgctc ccggagaagc tcgtctccac gccaagctcg
      181 tccaggaggg ccagcatggt gtagatatcc ttgatacgcg gcacgtggtg cagcgtggtc
      241 ttgcccggcg caaggagcgc ggcggcgacg aacttcaggg ctgcgttctt cgcccccgag
      301 acctcgacct cgccctccag gcgtctatta ccctcgacgt aaaatctctc agacacggat
      361 cctccccggt acgtgctcgt ctcccggtcc gtacgttcga actcgtaata ctactatgcc
      421 cccacaaggt tacgctacgg taacaacagt attacgactg cgttaatacc cgcggtaccg
      481 ctctccctcc gtgggtgcta gcgcccgagg gcgccagctc acggcgctag cttccgccgt
      541 acttgcgcgc caggcgtacc aggttctccc ccatgcgctg gcggcgctct atctcggcgg
      601 ttaccctatc acgatcctcg tcatcttccg agacctccga gagctcccgt tcggcctcct
      661 ccaccctcga ctcggccgcg tcgacgtcga tctcctcaac agagacggcc tcctcgacga
      721 gcacctgcac caggttttcg gagaccttga agaagccatc ggaggtggcg tagacgagct
      781 ggtcgtcgcc ctccttgatg cggacctccc caacctccac ggtggagaca agcggcgagt
      841 gatccaccag caccccgagg tcgccgtcgg cgatgcgggc tacgaccagg ttcgcctcgc
      901 cgtcgtagat gacctcctcc ggcgtgataa cccggcagat tagctgccga ccttccctgc
      961 gttggccgtt gtcctcagcc actatcgccc atcattctcg tcgttcccgc cggactcttg
     1021 ctccccggaa tcctcgtcgg agtcctcggt actggcctcc gcacgacccg agtcctcgga
     1081 ggtctcctcg tcggattcct cgcttgattc cacttcgtcc tcgtcgctca ggccccgcgc
     1141 cttctcgacc gcctcgtcta tggtgccgac gaggtagaag gcctgctcag gcaggtcgtc
     1201 gtgcttgccc tcgacgacct ccttgaacga gcgaatggtc tcgtccagct ccacgaactt
     1261 gccctccatg ccggtgaact gctcggcgac gaagaagggc tgggagagga agcgctgcag
     1321 gcgcctggcc cggccaacga tcaccttgtc ctcctccgag agctcgtcga tgccgaggat
     1381 ggcgatgatg tcctgaagct ccttgtagcg ctgcaggacg ttcttgacct cctgggcgac
     1441 cgcgtagtgc tcgtcgccga ccacggccgg gtcgaggatc gtggatgacg agctcaatgg
     1501 gtcgaccgcc gggtagatgc cctgggcggc aagcgagcgg gagagctcga tcgttgagtc
     1561 gaggtgggcg aacaccgtga acggggcggg gtccgtaaaa tcgtcggccg gcacgtatac
     1621 ggcctggaag ctcgtgatcg accctttctt ggtcgaggta atgcgctcct gcagggcgcc
     1681 catctccgtt ccgagcgtgg gctggtagcc gacctcggag ggcatacggc ccatcagggc
     1741 ggagacctcg ttgccggact ggacgaagcg gaagatgttg tccacgaaga agagcacgtc
     1801 ctggccgagc tcctcgcgga agtactcggc gagcgtaact cccgtcagcc cgacgcggaa
     1861 gcgggcgccg ggcggctcgt tcatctgccc gaacacgagc ccgacgttct ctaggatgcc
     1921 ggcctcctgg aactcgccgt agaggtcgtt gccctcgcgg gtgcgctcgc cgacgccggc
     1981 gaacacggag gtgccctcgt actgcagggc tatgttgttg atcagctcct ggataacgac
     2041 cgtcttgccc accccggcgc cgccgaagag gccgaccttg ccgcccctga gcacgggaca
     2101 gagcaggtct atgaccttga tgcctgtgac gaactgctcg gactgggtcg tgaggtcctc
     2161 gaacctcggg gcggggcggt gtatgggcca gtagtcctcg ggatcttcca cgggccccgc
     2221 ctcgtccacg gggtttccga gcacgtcgag cacgcgtccg agcacgttct tgcccacagg
     2281 caccgctatg gggcggccgg tgtccgtggc gtccacgccg cgcctgagac cgtcggtcga
     2341 ggccatggcc accgtgcgga ccatgtcgtc cccgaggagc tgctgcacct cgagggtgag
     2401 ctccgtggtc tcctcgttca cctcgagatc cacggtgacg gcgttgaaga tctccggtat
     2461 gtcctcaggc gagaaccgga cgtcgacgac cggtcccttg acctcgacga cggtcccgac
     2521 gcccgactta cgttcttcgc gtccgtccct accgtcgttt tcctgctggg cggcgtgacc
     2581 gttgtctacc tcgcccctct caccaccgaa ggtcgtccgg ttgaaggact gcccgctctc
     2641 gctctctgac atacaagttt cctttctcta gcccgcggag agggcctccg caccgccgac
     2701 gatctccagt atctcctgtg tgatctgggc ctgacgcgcc ttgttcatct gcaaggtaag
     2761 gtcgcgcacc agatcgttcg cgttgtccgt agcgttcttc atggccgtca tcctggcgcc
     2821 gtgctcaccg gcggcacctt ctagaagcgc ctgccacacc atcgtctcca cgtagcgcgg
     2881 gacgagcctc ttgagcaacg tctcggcgtc ggggacgtac tccatgtaag aggcgctgtc
     2941 gtcctgttcc tcgtcgcctt cctctaccac gggcaggagg cgtttcacga cgggggtctg
     3001 ggtcagcgcg ctgtcgaagc ggttgtagac caggtagacc tcgtcggcct cctcttcctc
     3061 gaagaggccc gtgagggcgg agccgatctc gcgcgccttc tcgtaggccg ggttgtcggt
     3121 gaagccggta aagacctcgg ccagttctac gcgcctgaaa cggaagaagg cgacggcctt
     3181 gcgaccgctc gcgacctgaa gcgcgttctg gtccttctcg ttcctgaggc cgacggtcct
     3241 acggagtact tgcgcgttga agccgccggc gaggccgcgg tccgcggtta gcgtgcagat
     3301 cgcgacgcgg ttcacctccc gccccacgag gagtgggagg tccccggagg cgctgccggc
     3361 cacgtcgcgc atcatctcct cgacgttctc ggcgtaggga cgggcctttt tcaccctggc
     3421 ctccgccttg cggaacttca ccgaggagac cgtctgcaag gccgagaaga ccctggactg
     3481 gttcttgacg gtgttgatct gccgctggag gtccctggcg gtggccaagt tctagccctc
     3541 ctcttcctcg tcgtctcctg gggacacctc gacgaggctc gtctgccccg gctcgaagtt
     3601 atcgttgaag ttctcgatgg cctcgtccag cttctgcccg ttctcgtcgg agagcttctg
     3661 ttcgtcgcgg atcgaatcca gcacctcgcc gtgggaatca cgcatgaagt cgcggaactg
     3721 gcgctcccag tccatcacca cgtcgggctc tacctcgtcc aggtagccgt tggacgcggc
     3781 gtagaggacc acgacctgct cctcgacggg catggggtcg agctccccct gcctcaggat
     3841 cgccgtcagg cgctcgcccc ggttgagggt ctgctgcgtc gccctgtcca ggtcgctccc
     3901 gaactgggcg aagctcgcca gctcgcggta ctggttcagg tccagacgaa gcgtaccggc
     3961 aacgtccttc atggccttga tctgcgccga agagcccacg cggctcaccg agttgccggt
     4021 gtcgatggcg ggacgctggc ccgagttgaa caggtcggag tcgaggaaga tctggccgtc
     4081 cgtgatggag atgacgttgg tcgggatgta cgccgagatg tccccggcct tggtctcgat
     4141 aaccggcaac gccgtgagcg aacctccgcc tgcggcgtcc gagagcttgg cggcccgctc
     4201 cagaaggcgg gagtgcaggt agaagacatc gccgggatag gcttcgcggc ctggcggacg
     4261 cctgagcaag agcatcatct ggcggtaggc gacggcgtgc ttggagaggt cgtcgtagat
     4321 gaggagcgaa tcctcgccgc ggttcatgaa gaactcgccg atggcgcagc ccgagtacgg
     4381 ggccaggtac tgcagcgtcg ccgactgggc cgccgaggcg ttcacgacag tggtgtactc
     4441 catcgcgccg gcctcttcca gggcggctac cacgcccgcc acggtcgagt ctttctggcc
     4501 tatggcgacg tagacgcact tcacgttctg gtcggcctgg ttgatgatgg tgtctagcag
     4561 gatcgaggtc ttgcccgtct tcctgtcccc gatcacgagc tcgcgctgcc cacgcccgat
     4621 ggggatcatg gcgtcgatgg ccttgatgcc cgtctggagc ggctgcttga ccggctggcg
     4681 cctgatgatg ccaggcgcca ctatctccac cggaagcgtc tcttcggctt cgatctcgcc
     4741 cttgccgtcc atcggacgcc caagggcgtc gatgacccgt ccgagaacgc cctcaccaac
     4801 gggtacgctc gcgaggcgct cggtgcgcgt cacggtgtta ccctcctgga tgtgggtgtc
     4861 gtcgccagcg atgatgacgc cgacgttgtc ctcctccagg ttcagggcga gaccggtgat
     4921 cttctccccg cgggaatcct cgaactccag catctcctgg tacatcgcgt tggtcaagcc
     4981 gtggacacgc gcgatgccgt ccccgacctc gagcacctgt ccgacctcct cggtcctgac
     5041 gcggttctcg taatccgtaa tagcgccttt caagatggag gagatctcct ccggcctgag
     5101 ccttcccact aaactacgct cctctcgagc atgccttctc ggaggctttc gagccgcccc
     5161 cgaatactgc cgtccatcat gcgccccgcg taccggaaga cggcgccccc gacgagcccg
     5221 gggtctacgt ttgtctccag gatgatttcc tggccttcga gtacccctct gagcctgtct
     5281 ctgagcctgt cgcgctgatc ctccgagagc tccacagcgg tcgttacctc gacctctatc
     5341 cttcccaggt gctgcttgac gagctcctcg tagcgggcga gtacctcttt cagtacctct
     5401 tcccgaccgt tgtccacgag cactttgaga aagttggtgg tcgaggtctt catgccttcg
     5461 gtcaggccgt cgagggcccg gcgcttctgg cgttcgggca cctgtccccc gtagaagaaa
     5521 agcctgagct cctcgctgtc gtgcagcgcc tcgacgaact ctccgaggtc agacaacacc
     5581 tcctcgagct cgtcccgctc acgcgcagcc ccgaagaggg cctcggcgta ggtggagacg
     5641 gcactcaagc ggttccgctc ctgcttccgg ccacttcgct ttcgaggtcg tcgagggcct
     5701 gggagatgag gcgctcgtgc tcgtcgcggt ctatggagcg gccgatgacc tgctcggagg
     5761 cctgcacgac catgtcggcg acctcgctac gtacctcgcg caccgccgat tcgcgctccc
     5821 tatctatctc ctcgcgggcg cgctggatga tgcggtcgcc ctcctcgcgg gcctccctct
     5881 tggtccgctc gcgctgggct tcggcctgct tgcgggcctc gtccaggatg cggcgcccct
     5941 ccccacgggc ctcctcgatc tggcgtctgt actcggccag gagctcgcgg gcctcggccc
     6001 gcgtcttctc ggcctcgtct atggactgct cgatctgggc ctgacggtcc tggatctgct
     6061 tgcggatcgg cgggtacacg taccggacca gcaagaacag caggatgaag aaagttatga
     6121 cctcccagaa gatcaggctg gtgttaccca gatcgaagat ccccgtcggg tccagttgtg
     6181 ctagcaccat tgccatgggc acccccttcc ccgcgctcct agagcacgaa ggcgatgagc
     6241 agggcgaaca cgatgccgta gagagcgaac gcctccacga gaccgatccc caggaacatg
     6301 agcgtcctgg tctggtcgaa ggcctcgggc tggcggtgga tggaggctat ggtctggccg
     6361 atcagatacc ccatcccggc ccccggaccg atgaccgcag ttcctgcggc gatgcctacc
     6421 ccgatcatgg tgacgtcgcc gctcgcctgc agcaacgcca gcatgggtac tacgagtgcc
     6481 aatacatcca tctttgctcc tttcgcctct cggctgactc tatcgactta cctttgacgc
     6541 ttcactagtg atcacggaac atcgccagct cgatgtaaac ctgcgtgagt atagcgaata
     6601 tgtatgcctg caaaaccgcc acgaagatct cgaacccgta gaacaccacg gataggggca
     6661 cggagacgac cgcgacgatc gtgccgaagt acaggatgag ccccaggaac acgtagatga
     6721 tgaggtgccc ggccaggatg ttggcgtaga gccgcatccc gagcgtcaag ggcttggtga
     6781 actcgcccag tacctcgatg ctccacatga acggcaccat cacgggcttg agcggcaagc
     6841 cgggtggcgc gaagctcttg aggtacgctc cagccccgtt gcgcctgaac ccctcgtact
     6901 gcgtgag
//
LOCUS       DBNGVD010000504         9034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2312689, whole genome shotgun sequence.
ACCESSION   DBNGVD010000504 DBNGVD010000000
VERSION     DBNGVD010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2312689"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="AC16CM_13615"
     CDS             complement(<1..1138)
                     /locus_tag="AC16CM_13615"
                     /inference="COORDINATES: protein motif:HMM:NF021098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIR3140917.1"
                     /translation="MAQSPAVHIELRDDAWIVIREGNQRATSVHPTQAEAAESGRELA
                     RAAGAQFFLHGTDGRVREHHDYSGDRTAQDEGFLDTPLGTVGTVTEAADGITSTAAQT
                     LGADTTSDPAPAREKPAFGSPRTTGDTGDVIPPAVGSTITPEERYADYEVYDQYGQRI
                     GPISDLFVDDNDEPEYVGVEPGTMKDRSVLVPGEVISINDGLRRMVVSRPITVVETAP
                     SLGHDEQVTPDFERRVRLHYALPLANETVRPGQPGAPVAPAERSERAEAVQTGDPDPL
                     SRVTGREDDEVTVQRVEEELLVGRREREAGSMRIRKRVRTETEHLEVPRKRVEVTVER
                     VPVDETTASDTGITSTPQIEEEEIVVPIVEEEILIEKRPVVKEEI"
     gene            1366..2415
                     /locus_tag="AC16CM_13620"
     CDS             1366..2415
                     /locus_tag="AC16CM_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3140918.1"
                     /translation="MEYRRLGRSGLYVSAMTLGTMTFGGRGDFGMVGSTDVATATRQV
                     DACLDAGVNLFDTADMYSDGESEEILGQAISGRRDRLLLATKARMPVGDGPNETGLSR
                     QHLIRQCEASLRRLGTDYIDLYQTHEWDGITPLDETLEALDTLVRSGKVRYIGSSNLS
                     GWQLMKALGVSERLGLQRYVSQQIYYSLEARDAEYELVPLAVDQECSILVWSPLAGGL
                     LSGKYRRDEDAAEGRHVSGWDEPPVRDPEKLYDTIDVLVEIADGHGVSAAQVSLAWLL
                     GRPGVASIVVGARTEEQLADNLAAADLNLSDEERARLDEVSAPPLLYPYWHQARTASD
                     RLSPADLTLLSPHLD"
     gene            complement(2518..3363)
                     /locus_tag="AC16CM_13625"
     CDS             complement(2518..3363)
                     /locus_tag="AC16CM_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3140919.1"
                     /translation="MELSIAPYVRDASIPVRSAWYTARRRLLDYLIMYVQEGEMIAHV
                     EGRGYEFGAGEFCLIQPGEVHTLEGPAHTITPYLHLDVFHNSGREQSFVVRSGRVDLS
                     GHGDLMQPRLEEALGVRVPTGFVPPHPAQFRDTMLKAIGIWQRRDFLGQLEAQYLAMG
                     LVLSILRGYSSESSESSDEPEFLNWITSYMSLHLSEPLTISDMAARAGLSASRFSQVF
                     RNRFARPPHQFLLHLRVQRAQDLLHHTSLTMREISAQCGFSDVHHFAKTFKRLSGQTP
                     GSYRR"
     gene            3635..5014
                     /locus_tag="AC16CM_13630"
     CDS             3635..5014
                     /locus_tag="AC16CM_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3140920.1"
                     /translation="MHAHISRRQFLARGGKAALGVGLAGSLLSGCGGGGGGGGQVTYW
                     SNLEGSGPQAYFKKSIEKPFEKANGGTDLKVTFQPPDEMDRLVRTALQGGEGPDIIMT
                     PGPAYAQEYIDANLFAELDDYSSEYKWEDKLLGWALDLGRYEDKLYAIPYQLQTMLLY
                     YNKTLFEDKGMAPPKNRDELEAMAEELQGQGITPFAAGIGDDPAAIEWFPTVFWNHYS
                     GPDALYQALTGEIPFSDPIFVEAIDLFNSYVQKGWFGGSQEKFFSNGFDALHADLGDG
                     KAGTNVEGSWFLATINDYFENSGDAWDWAPLPPLRSGVPAELYELGIGSTLSVNGRAE
                     NAEGAAAFIDYLVADKKRAAEWMAAVPSAFNAPLPFSESDFPSSMDERVARQLAALSQ
                     ATNQGNFGYTAWTFWPSKSDVYIQEEMQKVLTGDITAAEFCRGLDEQFTEERKEGAVP
                     KIIKPGGAA"
     gene            5014..5925
                     /locus_tag="AC16CM_13635"
     CDS             5014..5925
                     /locus_tag="AC16CM_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3140921.1"
                     /translation="MAQGAAKSASTGGLRRRRLLSAWLFMVPLLVVNLLVILGPSVAT
                     VYYSFTEWSGIGPAEWVGLQNYRDIVADVDFWSALLHNLIWTMIFLTVPIGMGLLGAF
                     LLSQITRFQMIFRVLYFIPYVIASVVNAALWQNILDPSRGIGSALATVGIPFLDGVSF
                     FGSERLALPAVAFVDNWHWWGFVVLLFLTALQSVDKELYEAAKMDGANRWQQFINVTI
                     PGIRATLVFVVLITIIGSLLVFDYIYIITQGGPAGASEVVGTLMYKEAFARFEAGYAA
                     AMGLGLSFMSGLVIVVFLFLRRRGWEI"
     gene            5913..6773
                     /locus_tag="AC16CM_13640"
     CDS             5913..6773
                     /locus_tag="AC16CM_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3140922.1"
                     /translation="MGDLSTPARGRRQRREPSKFPYYIVLVLLGLFAVGPLVVLIFNS
                     LKTNAEIGRNPLGPPTSPVFENFATAWTQGDFATTLVNSTILTTGTIVGVCVIAGTAA
                     YAMARLDLPGTNAVLLYLFVASALPFQLFIVPLFFLWSTLQLTNTLYGLIVIYWAIFS
                     PFATLLLRSYLVALPRGFEDAARVDGANELQVLTRVVLPLSWPGFLTIALVSGLGAWN
                     EFFFAITFIQDQNLKPVTTSFLAFQSNFSRDWGLTSAASLIIILPVLVLFLGLQRRFI
                     NGLTAGGLKG"
     gene            6770..7624
                     /locus_tag="AC16CM_13645"
     CDS             6770..7624
                     /locus_tag="AC16CM_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3140923.1"
                     /translation="MKRGFLYSYNVIGFGGEDVAMSIDRLARLGYDAVEIEGEPEGHD
                     PGRIRKLAADAGLSVASVCPNFTEERDLSHPDQERRSTAVAYLRSVAEFAAEAGAPLF
                     IVAPTAYARVRPVADPLDEWLWAVEGIRDAGNHASSLGVDLSLECWNRYGTYMLNRLE
                     EGAKMWQETGLANGGVMADTFHMNIEERSISDAILEHAGVLNHVHLSDSNRLAPGLGH
                     IDFAEVLRALTEVGYDRSLAFELIPALPNLLEEGRDAPSLDGVAEQALRHMVQVEQLI
                     SAGGSGSS"
     gene            7656..8711
                     /locus_tag="AC16CM_13650"
     CDS             7656..8711
                     /locus_tag="AC16CM_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3140924.1"
                     /translation="MPRQLVAVAPRKAALVDYDEPEIGSRQIRVRVEYASPKHGSEIG
                     VFRGEDPFVADLFDEDWRLFVTRKDARIASDGGGPVLGNQWVGIVVERGEEVRRFAVG
                     DRVCGYGGIRETQMLNADTEPFLLPVPDGMPWKSALCLDPAQFALGGIRDGRVRLGDR
                     VAVFGLGAIGAIAAQMARVAGADYVAVIDPIQKRRDAAVAAGADAALDPVSEDVGLEL
                     KRATSRAGVDVAIETSGNEQALQQALRGLGYGGTIAFVGWARAFSGTLDLGREAHFNN
                     ANLVFSRASSEPSRDHPRWNRQRIAATCWNLLSSNQIDCQRIIDPVVPFPQAPDAYEQ
                     YVDRNPQESIKLGIAFE"
     gene            8820..>9034
                     /locus_tag="AC16CM_13655"
     CDS             8820..>9034
                     /locus_tag="AC16CM_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140925.1"
                     /translation="MARELTNLNTYSIDDVIRVPIVLHDEDGVAHVRAVFRRLRYAGE
                     LGPRGLDPDDTMELRGNGNGQKRATVEV"
BASE COUNT         1610 a         2878 c         2887 g         1659 t
ORIGIN      
        1 ggatctcttc cttcaccacg ggtcgcttct cgatcaggat ctcttcctcg actatgggga
       61 cgactatctc ttcttcttcg atctgcgggg tcgaggtgat gccggtgtca ctcgcggtag
      121 tctcatctac cggcacgcgc tctacggtta cctcgacgcg cttcctgggc acctccaggt
      181 gctcggtctc ggtgcgcacg cgcttgcgga tgcgcatgct cccggcctcg cgctcacgcc
      241 tgccgacgag gagttcctcc tccacacgct gcaccgtcac ctcgtcgtcc tcgcggccgg
      301 ttacgcgcga cagagggtcg gggtcacccg tctgaaccgc ctcagcccgc tccgaccgtt
      361 ccgccggcgc gaccggtgcg cccggctgcc ccggtcgcac ggtctcattc gccaggggca
      421 gggcgtaatg gagcctgacc ctccgctcga aatctggggt cacctgttcg tcgtggccca
      481 ggctcggcgc ggtctcgacc acggtgatcg ggcgtgagac aaccatccgc ctgagcccgt
      541 cgttgatgga gataacctcg ccggggacga ggacggagcg gtccttcatg gtgcctggct
      601 ctacgccgac atactctggc tcgtcattgt cgtcgacgaa caggtcactg atcggcccga
      661 tcctctgccc gtactggtca tagacctcgt agtccgcata ccgctcttct ggcgtaatcg
      721 tggagcctac ggcgggtggg atcacatccc cggtatcccc ggtcgtacgg ggggagccga
      781 aggccggctt ctccctggcg ggggcgggat cactggtcgt gtcggcaccg agggtctggg
      841 ccgcggtgga ggtgatccca tccgcagctt cggttacggt gccaacggtg ccgagtgggg
      901 tgtccaggaa gccctcgtcc tgcgcagtcc ggtctccgct gtagtcgtga tgctctctaa
      961 cccggccgtc cgtcccgtgg aggaagaact gggccccggc cgcacgggcc agttccctgc
     1021 cgctctctgc cgcctcagcc tgggtcggat ggaccgaggt cgcccgctgg ttgccctcac
     1081 ggatcacgat ccacgcgtcg tccctgagct cgatgtgcac ggcaggggat tgagccatat
     1141 cctccacgtc ctttcgttct actgcattgg ccgcgtttac gcctgtttgc ctatcttact
     1201 ctacccacgg gtgggtcttc ccaacactgg gcctataaga gattagatgt agacgggaac
     1261 agaagggtta cgccgggccg tttctggagc ggtcagacgg cgaatgccat cccggcgtgc
     1321 taggctgacg tctgacccgg tagcagattt tttgggagga ttctcatgga gtaccgccgt
     1381 cttggacgct ccggacttta cgtctcagcc atgactcttg gcacgatgac cttcggggga
     1441 cgtggcgatt tcgggatggt ggggtctacg gacgtggcga cggcgaccag gcaggtggac
     1501 gcttgcctcg acgcgggcgt caacctcttc gacacggccg acatgtactc agacggcgag
     1561 tccgaggaga tcctcggtca ggccatctcc ggccggcgtg acaggctact gctcgcgacc
     1621 aaggcgcgca tgccggtggg ggatggacca aacgagaccg gcctctccag gcaacacctg
     1681 atccgtcagt gcgaggcgag cctcaggcgt ctcggcacgg actacatcga cctctaccag
     1741 acgcacgagt gggacggcat aacccccctc gatgagacgc tagaggcgct cgacacgctg
     1801 gtccgttctg gcaaggttcg ctacatcggc agctcgaacc tctccggctg gcaactgatg
     1861 aaggcgctcg gcgtctctga gaggctgggc ctgcaacgct acgtgagcca gcagatctac
     1921 tactccctcg aagccaggga cgcggagtac gagctcgtcc ccctggccgt ggaccaggaa
     1981 tgctccatcc tggtgtggag cccgctggcc gggggactcc tctccggcaa gtaccgccgt
     2041 gacgaggacg ccgccgaggg acgccacgta agcggctggg acgagccgcc ggtccgggac
     2101 cccgagaagc tctacgacac catagacgtg ctggtcgaga tcgccgacgg tcacggcgtc
     2161 tccgcggcgc aggtttccct cgcctggctg ctaggccgtc ccggcgtggc ctccatcgtc
     2221 gtcggcgccc gcaccgagga acagctcgcg gataacctcg ccgccgccga cctgaacctc
     2281 tccgacgaag agcgcgcccg cctcgacgag gtgagcgccc cgccgctcct ctacccctac
     2341 tggcaccagg cccgtacggc ctccgaccgc ctgagcccgg ccgacctgac cctcctcagc
     2401 ccgcacctgg actagggtag ctatcagccg tcagcttata gctttcagct ctctgttctt
     2461 gcttacggct gaagatcgaa agatctcctg gcggatgatc gatatttccg caaggcgcta
     2521 cctccggtaa gagcctgggg tctggccgga gagcctcttg aaggtcttgg cgaagtggtg
     2581 cacgtccgag aacccgcact gggccgagat ctccctcatg gtgaggctcg tgtggtggag
     2641 caggtcctgg gcgcgctgca cgcgcaggtg gagcaggaac tggtggggcg gcctcgcgaa
     2701 tcggttgcgg aagacctggg agaatcggga ggccgagagg ccggcccggg cggccatgtc
     2761 cgagatggtg aggggctcgg agagatgcag cgacatgtag gaggtgatcc agttcaaaaa
     2821 ctccggctca tcggaggact cggagctctc gctggagtag ccacgcaaga tggacagaac
     2881 aagccccatc gccaggtact gggcctctag ctggccgaga aagtccctgc gctgccagat
     2941 gcctatggcc ttgagcatgg tgtctctgaa ctgcgccgga tgcggcggga cgaaccccgt
     3001 cggaaccctg acccccaacg cctcttcgag acggggctgc atgaggtccc cgtgcccgga
     3061 gaggtccacc cgcccggaac ggaccacgaa gctctgctcc cgcccggaat tgtggaacac
     3121 gtctagatgc aagtagggcg taatagtgtg ggcgggaccc tccagggtgt gtacctcgcc
     3181 aggctggatc aggcaaaact ccccggcccc gaactcgtac ccacgcccct cgacatgcgc
     3241 gatcatctct ccctcctgaa cgtacatgat gaggtagtca agaagacgtc gcctggccgt
     3301 gtaccacgcg ctcctcacgg gaatgctggc gtcacggacg taaggtgcta tggataattc
     3361 caagagaaac catcccttct gcctccaact ccagatcttt ctacaggtgt tttttacaac
     3421 aactcaggac gtgtaagcaa tgattcaagc tattgtgtgc cttaatatgc gggcgaaacg
     3481 ggtgggagca aacggatcct tcgcacaggg gtgtgtagta gagggatgga aaagaacaaa
     3541 gccgatctcc ggcgaaccgc ttcctgccgt gctcgtaaag agggtaccct cggtgctcga
     3601 ccgttcggtg tcaacgtctg aaaggtagct ggtcatgcac gcacacatct cgcgcaggca
     3661 atttctggca agggggggaa aggcggccct cggggttggc ctggcgggct cgttgctctc
     3721 cgggtgcggg ggcggcgggg gaggcggcgg ccaggtcacc tactggagca acctcgaagg
     3781 ctcggggccc caggcgtact tcaagaagag catagagaaa cccttcgaga aggcgaacgg
     3841 gggcacggac ctcaaggtta cgttccagcc gccagacgag atggaccggc tcgtcaggac
     3901 ggccctgcag ggcggcgagg ggcccgacat tatcatgact ccggggccgg cgtacgcgca
     3961 ggagtacatc gacgccaacc tgttcgccga gctcgacgac tactcaagcg agtacaaatg
     4021 ggaggacaag ctccttggct gggcgctcga cctcgggcga tacgaggaca agctgtacgc
     4081 catcccgtac cagctccaga ccatgcttct ctactacaac aagaccctct tcgaggacaa
     4141 gggcatggcg cccccgaaga accgcgacga gctcgaggcc atggccgagg agttgcaggg
     4201 gcagggcatt accccgttcg ccgcaggaat cggggacgac ccggcggcca tcgagtggtt
     4261 cccgaccgtg ttctggaacc actactcggg tccggacgcg ctctaccagg cgctgaccgg
     4321 ggagatcccc ttctcggatc ccatcttcgt ggaggccatc gatctcttca acagctacgt
     4381 ccagaagggc tggttcgggg ggagccagga gaagttcttc tccaacggct tcgacgccct
     4441 gcacgccgac ctcggcgacg gcaaggccgg gaccaacgtg gagggctcct ggttcctggc
     4501 gaccatcaac gactacttcg agaactcggg agacgcctgg gactgggcgc cgcttccgcc
     4561 cttgaggagc ggcgtgccgg ccgagctgta cgagctcggc atcgggtcca ccctctccgt
     4621 caacggcagg gcggagaacg ccgagggggc cgccgcgttc atcgactacc tcgtggcgga
     4681 caagaagcgg gccgccgagt ggatggccgc cgtgccatcc gccttcaacg cgccgcttcc
     4741 cttcagcgag tcggactttc cctccagcat ggacgagagg gttgccaggc aactcgcggc
     4801 gctcagccag gccaccaacc agggtaactt cggttatacg gcgtggacgt tctggccctc
     4861 caagtccgac gtgtacatcc aggaggagat gcagaaggtt ctaaccggcg acatcacggc
     4921 ggccgagttc tgccgggggc tcgacgagca gttcacggaa gagcgcaagg agggcgccgt
     4981 gcccaagatc atcaagccag ggggcgccgc atagtggcgc agggcgcggc caagagcgct
     5041 tccacgggcg gcctgaggcg gcgtaggctt ctgtcggcct ggctcttcat ggtcccgctg
     5101 ctagtcgtca acttgctcgt catactcggc ccttcggtcg ccacggtcta ctactccttt
     5161 acggagtggt cgggcatcgg cccagccgag tgggtcggcc tccagaacta cagggacatc
     5221 gtcgccgatg tcgacttctg gtccgccttg ctgcacaacc tgatctggac gatgatcttt
     5281 ctgacggttc caatcggtat gggcttgctc ggggcgttcc tgctctctca gatcacgcgc
     5341 ttccagatga tattccgcgt cctgtacttc atcccttacg tgatcgcgag cgtcgtgaac
     5401 gccgcgctct ggcagaacat tctggacccc tcacgtggca tcggttccgc gcttgcgacg
     5461 gtaggcatcc cttttctcga cggggtctcc ttcttcggga gcgagaggct tgcgctacct
     5521 gctgtcgctt tcgttgacaa ctggcactgg tggggctttg tcgtactgct ctttctgacg
     5581 gccctgcagt cagtagacaa agagctctac gaggcggcca agatggacgg ggcgaaccgt
     5641 tggcagcagt tcataaacgt caccataccg ggtatcaggg cgacgctcgt cttcgtcgtc
     5701 ctcataacca tcatcgggtc cctcctcgtc ttcgactata tctacatcat cacccagggc
     5761 ggaccagcgg gggcgagtga ggtcgtgggc accctaatgt ataaagaggc cttcgcccgc
     5821 ttcgaggccg ggtacgcagc ggctatgggg ctcggtctga gctttatgag cggcctggtc
     5881 atcgtggtct ttctgttcct gcggagaagg gggtgggaga tttgagcacc ccggccagag
     5941 gacgacgtca gagacgggag ccttcgaagt ttccttacta catcgttctc gttttgctcg
     6001 ggctattcgc cgtcgggccg ctggtggttc tgatcttcaa ctcgctgaaa acgaacgccg
     6061 agatcgggcg caaccctctt ggtccgccga cctcgccggt gttcgagaac ttcgcgaccg
     6121 cctggacgca gggtgatttc gcgacgaccc tcgtaaacag cacgatcctg accacgggca
     6181 ccatcgtcgg ggtctgcgta atcgccggca cggcggccta cgcaatggcg cgactggacc
     6241 tgcccggcac gaacgcggta ctgctctacc tcttcgtggc gagcgcgcta ccgtttcagc
     6301 tctttatcgt gccgctgttc ttcctgtgga gcacgctaca acttaccaac acgctctacg
     6361 gcctgatcgt gatctactgg gcgatattct cgccgttcgc caccctgctc ctgcgctcct
     6421 acctagtggc cctgccgcgc ggcttcgagg acgcggcccg ggtagacggg gcgaacgagc
     6481 tgcaggtctt gaccagggtg gtgctgccct tgagctggcc aggcttcctg acgatcgccc
     6541 tcgtctccgg gctcggtgcg tggaacgagt tcttcttcgc catcacgttt attcaggacc
     6601 agaacctcaa gcccgtcacg acgagcttcc tcgcgttcca gagcaacttc agccgcgact
     6661 ggggtctgac cagcgccgcc agcctcataa tcatcctgcc cgtcttggta ctgtttctcg
     6721 gcctccaacg gcgcttcatt aacgggctca ccgccggtgg tctgaaggga tgaagcgggg
     6781 gttcctctac tcgtacaacg tgatcggctt cggcggcgaa gacgtcgcca tgtccattga
     6841 ccggcttgcc aggctcggct acgacgctgt ggagatcgag ggagagccgg aggggcacga
     6901 tcccgggagg atacgcaaac tggctgcgga cgcgggcctc agcgtggcct ccgtgtgccc
     6961 caacttcacc gaggagcgcg acctctccca tcccgaccag gagaggagaa gcaccgccgt
     7021 cgcctacctg cggagcgtcg ctgagttcgc ggcggaggcc ggagccccgc ttttcatcgt
     7081 cgccccgacc gcgtacgcca gggtcaggcc cgtcgccgat cccctcgacg agtggctgtg
     7141 ggccgtggaa gggatcaggg acgccgggaa tcacgcctct tcgcttggcg tcgacctctc
     7201 gctggagtgc tggaaccgct acggcaccta tatgctgaac cggctggagg agggggccaa
     7261 gatgtggcaa gagacaggcc tcgccaacgg gggtgtcatg gcggacacct tccacatgaa
     7321 catagaggag cgttccatct ccgacgccat cctggaacac gctggcgttc tgaaccacgt
     7381 tcacctctcc gactccaacc gcctggcccc cggcctcggg cacatagact tcgcggaggt
     7441 acttcgggcg cttacagagg taggctacga caggagcctc gccttcgagc taatcccggc
     7501 gctgccgaac ctgctggagg aagggagaga cgctccatcc ctcgacggcg tggcggagca
     7561 ggcccttcgc cacatggtac aggtcgagca gctgatctcc gccggagggt cggggtcatc
     7621 ttgaagaaca tcgtggcatc gggaagaggg ctctgatgcc gaggcagctg gtcgccgtcg
     7681 cgccaaggaa agccgccctc gtggactacg acgagccgga gatcggaagc cgtcagatcc
     7741 gggtccgtgt cgagtacgcc tccccgaagc atggctccga gataggcgta tttcgtggcg
     7801 aagacccttt cgtcgccgat ctcttcgacg aggactggcg actctttgta acccgcaagg
     7861 acgccagaat cgcgagtgat ggtgggggtc cggtcctggg caaccagtgg gttggcatcg
     7921 tagtcgagag gggagaggag gtgcggcgct tcgcggtcgg ggaccgcgtg tgcggctacg
     7981 gtggcataag ggagacgcag atgctgaacg cggataccga gcccttcctc ctgcccgtgc
     8041 cagatggcat gccgtggaag agcgccttgt gcctcgaccc cgcccagttt gccctcggcg
     8101 gcatccgcga cggccgggtg cgcctcgggg accgtgtcgc cgtgttcggc ctcggcgcga
     8161 taggcgccat agcggcccag atggccagag tggccggcgc agactacgtg gccgtcatcg
     8221 accccatcca gaagcgaagg gacgcagccg tggccgctgg cgcggacgcg gccctcgacc
     8281 ccgtgagcga ggacgttggc ctcgagctca agcgggccac cagcagggcc ggggtcgacg
     8341 tggccataga gaccagcgga aacgagcagg cactgcagca ggccctgagg ggcctcggct
     8401 acggcggcac catagccttc gtcggctggg cgcgggcctt cagcgggacc ctggatctcg
     8461 gcagggaagc ccacttcaac aacgccaacc tcgtcttctc ccgcgccagc agcgagccga
     8521 gccgggacca cccgcgctgg aaccgacagc ggatagccgc aacctgctgg aacttgctct
     8581 cctccaacca gatcgactgc cagagaataa tagatccggt agtgccgttc ccccaggccc
     8641 ccgatgccta cgagcagtac gtagaccgca acccgcaaga gagcatcaag ctaggaatag
     8701 cattcgaata gaggcaacag gcaacaggta atattgctga agcctgaaac cttccctctc
     8761 ttgtttcagg agacgcgttt cgaggaacat caattgtgct gtctttatgg gaggttgcaa
     8821 tggcgcgaga actgaccaat ctgaacacgt actccataga cgatgtgatc cgggttccca
     8881 tagtcctgca cgacgaggat ggcgtggccc acgtcagggc cgtcttccgg cgcttgaggt
     8941 acgccgggga acttggcccg agggggcttg acccggacga caccatggag ctgagaggca
     9001 acggcaacgg acagaagagg gccaccgtcg aggt
//
LOCUS       DBNGVD010000505         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_855385, whole genome shotgun sequence.
ACCESSION   DBNGVD010000505 DBNGVD010000000
VERSION     DBNGVD010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_855385"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16CM_13660"
     CDS             <1..917
                     /locus_tag="AC16CM_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3140926.1"
                     /translation="FNCNAPDTGNMEVLVRYGTEEQKERWLRPLLDGEIRSCFAMTEP
                     DVASSDATNIQASIERDGDEYVLNGRKWWTSGAGDPRCKISIFMGKTDPEASRHEQQS
                     MILVPLDTPGVRVERTLPVFGYDDAPHGHAEISYDDVRVPVENIIWGEGKGFAIAQGR
                     LGPGRIHHCMRLIGVSERALGEMCERVKTREAFGRPLAEQGVVQAWISDSRMEIEQAR
                     LLTLKAARMMDTVGNKEARAEIAMIKVVAPNVALNVLDRAIQAHGGAGVSDDFPLAYA
                     WASARTLRLADGPDEVHRAAVARLELKK"
     gene            950..1195
                     /locus_tag="AC16CM_13665"
     CDS             950..1195
                     /locus_tag="AC16CM_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140927.1"
                     /translation="MLLGIKYEAARIGEVDAAALAGIEEQMKREVGESWASDDEVLRG
                     RNAVRREHGLPGLGGPGDRPTPPAGGRRPLWRRLLRR"
     gene            1198..2058
                     /locus_tag="AC16CM_13670"
     CDS             1198..2058
                     /locus_tag="AC16CM_13670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3140928.1"
                     /translation="MLYGNFATQEELDAQYNLTAMLPEAAARYETFCWRESEAVRGEL
                     EHSLDVSFGPTRAEHVDVYPAGEKTPVLVYVHGGFWCLRTSKEFGFTARGPVSRGVAT
                     VVTNYDLCPRVTMDEIVRQTRAAVAWAYKNAAGFGGDPDRIHVAGHSAGGHLVAMLLA
                     TDWPGEYGLPADIIKGATMVSGIYDLSPLPYTFLQPTLQLGYDGIYRNSPVLRLPNTT
                     APLLIAYGDQETDEFKRQSEDYLEALRVGGFEVARLILEGKNHYEVIDGFLDAGSPLC
                     SAILAQMGVQ"
     gene            2090..2305
                     /locus_tag="AC16CM_13675"
     CDS             2090..2305
                     /locus_tag="AC16CM_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140929.1"
                     /translation="MATEMTGGKIVGERGTVVTFRQRCEACGYVFDWNKTTIVPAYGS
                     RKVRSFTCPECGNYQEVEVRYLHKGPR"
     gene            complement(2378..>2685)
                     /locus_tag="AC16CM_13680"
     CDS             complement(2378..>2685)
                     /locus_tag="AC16CM_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140930.1"
                     /translation="LGPHPMPDLVEQIALDSITEGLLYGELLENTPENFPESARFMVA
                     DRMRRGKLIAGPRSDYGFRYNGTLNGRKVDEFKAALVQRVFRMADAEGLTWTWKCNY"
BASE COUNT          514 a          845 c          894 g          432 t
ORIGIN      
        1 tcttcaactg caacgccccc gacacgggca acatggaggt gctcgtccgc tacggtacgg
       61 aggaacagaa ggagcggtgg ctgcggcccc tgctagacgg ggagatcagg tcttgcttcg
      121 ctatgaccga gccggatgtg gcctcttccg acgcgacgaa catccaggcg agcatcgagc
      181 gagacgggga tgagtacgtc ctgaacggca ggaagtggtg gacctcgggg gccggtgacc
      241 cgcgctgcaa gatctccatc ttcatgggca agacggaccc ggaggcgtcg cgccacgagc
      301 agcagtccat gatcctggtc ccgcttgaca cgcctggcgt gcgggtagag cgaaccctcc
      361 cggtctttgg ctacgacgat gcgccgcatg ggcacgcgga gatctcctac gatgacgtac
      421 gtgtgcccgt ggagaacatc atctggggcg agggcaaggg cttcgccatc gcgcagggac
      481 gcctgggacc gggtcgcatc caccactgca tgcgcctgat cggcgtgtcg gagcgggccc
      541 ttggggagat gtgcgagcgg gtcaagacca gggaggcctt cggcaggccg ctcgcggagc
      601 agggcgtcgt gcaggcctgg atctccgact ccaggatgga gatagagcag gcccgcctcc
      661 tgaccttgaa agccgcacgt atgatggata cggtagggaa caaagaggcc agggcggaga
      721 tagccatgat caaggtggtg gccccgaacg tggccctgaa cgtgctggac cgggcgatac
      781 aggcccacgg tggcgccggc gtctcagacg attttccgct tgcctacgcc tgggcgagcg
      841 cacgcaccct gcgtctcgcc gacggtcccg atgaggtcca ccgggccgcg gtggccaggc
      901 tcgagctaaa gaagtagtag aggaggatga catccagaac aaagactccg tgctcctcgg
      961 catcaagtac gaagcggcga ggattggcga agtggacgct gccgccctcg ccggcatcga
     1021 ggagcagatg aagcgggagg ttggcgagag ctgggcctcc gatgacgagg tcctgcgcgg
     1081 acgcaacgcc gtacggcgcg agcacggcct ccccggcctc gggggacccg gcgacaggcc
     1141 aacccctccg gccggcggtc gacgcccgct ctggcgccgg ctcctgagaa gatagcgatg
     1201 ctctacggaa acttcgcaac ccaggaagaa ctggacgccc agtacaacct gacggccatg
     1261 ttgcccgagg cggcggcccg ctatgagacg ttctgctggc gggagagcga ggccgttcgg
     1321 ggggagctcg agcacagcct ggacgtgtcc ttcggcccca cccgcgcgga gcatgtcgac
     1381 gtctatccgg cgggggagaa aacccccgtg ctcgtctacg tgcacggcgg cttctggtgc
     1441 ctgcgcacga gcaaggagtt cgggttcacg gcgcggggcc ccgtatccag gggcgtcgcg
     1501 accgtggtca cgaactacga cctctgcccg agggtgacga tggacgagat cgtgcgccag
     1561 acccgcgcgg ccgtggcgtg ggcctacaag aacgccgccg gcttcggcgg cgaccccgac
     1621 cgtatccacg tcgccggaca ctcggcaggg ggtcacctcg tggccatgct cctcgcgacc
     1681 gactggcctg gtgagtacgg cctgccggcc gacatcataa agggcgcgac tatggtcagc
     1741 ggcatctacg acctctcgcc cttgccctac accttcctgc agcccacgct ccagctcggg
     1801 tacgacggca tctacaggaa tagccccgtc ctacgcctcc cgaacaccac ggcacccctg
     1861 ctcatcgcct acggagacca ggagaccgat gagttcaagc gccagtcgga ggactacctg
     1921 gaagcgctga gggtgggagg cttcgaggta gcgcgtctga tcctcgaggg caagaaccac
     1981 tacgaggtga tcgatggttt cctcgacgcc ggaagcccgc tctgctccgc gatcctggcc
     2041 cagatgggcg tacaatgagc aggagagcac gcacgacccc ggaggacgaa tggcgacaga
     2101 gatgacgggc ggcaagatcg tcggcgagcg cggcacggtg gttaccttca ggcagcgctg
     2161 cgaggcctgc ggctacgtgt tcgactggaa caagaccacg atcgtgccgg cctacggctc
     2221 ccgcaaggtg cgttcgttta cctgccccga atgcggcaat tatcaagagg tcgaggtccg
     2281 ttacctccac aaaggtcccc gttgagagct caattgacct ccggggtgcc gtttttccgc
     2341 gccagcaata acggcccgtg agatcgagcg tcttgtatca atagttgcat ttccaggtcc
     2401 aagtcagacc ctcagcatct gccatcctga aaacgcgttg gaccaaggcc gccttgaatt
     2461 cgtccacttt gcggccgttc agagtgccat tgtatctaaa cccgtagtcc gatcgcggac
     2521 ctgcaatcag cttgccccga cgcatccggt cagcgaccat gaagcgcgcg gactccggga
     2581 agttctcggg cgtgttttcc agaagctcgc cgtaaagcaa accctctgtg atggagtcca
     2641 gggcgatttg ctccaccaag tcgggcatgg gatggggtcc gagaa
//
LOCUS       DBNGVD010000506         2914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4863996, whole genome shotgun sequence.
ACCESSION   DBNGVD010000506 DBNGVD010000000
VERSION     DBNGVD010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4863996"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..2276)
                     /locus_tag="AC16CM_13685"
     CDS             complement(420..2276)
                     /locus_tag="AC16CM_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140931.1"
                     /translation="MSRLRRIKVTQHTPVRSAAKSAGRATFGTFSDAMKRLVVDDLRP
                     GVVHVGDLPGPARALAWLGFILVFTGTGMLFFNEPLREAFPLLPMVGGTTGRGELVPF
                     VLIPVTIFILSVAWGFMLAGALHSRLLVKLAVLLLYLVVASVWTASTVTGIPAALVSV
                     GALLVVPVFFFLRRKAAEAPAFEFGVLLVLVALTFGVSQIYAIDAWRSSGVPGVLANV
                     PTLLYTFDMLVIPLMLYLGVDIAEFVHKTASWTSGLADIWAGRRVMYAILALLLGWRL
                     VWVVMEAAGRIGKSSLADQAAAYAGALVIPLSVGMVWWVVRRYGGDPVTVETLLDVAK
                     RVALPLIAIYLGLQGIEIVVVLIGGLLGLLLVRVGLAGPGDPGGILALAEGLIGQDAN
                     WQTLVAGVAIVAAVFLARRGRPTLALYLGIVGANALWISLTNPGRPLSFFAWSGLEPV
                     DFWWVVIFASVAVYWAARRTLTEIRARRLVLLVLITLLMRQTGFIEDPFSPVLGFTGV
                     GMIAIGLIWDALTIGFWANEGSRGLPRASRIWLYLGYILFSIAVLNWAVASHDILQVD
                     FFTGQAALGGFGIFGKPLIYAIFAVILARPDVEETEADPAREPDREAQQATP"
     gene            complement(2273..>2914)
                     /locus_tag="AC16CM_13690"
     CDS             complement(2273..>2914)
                     /locus_tag="AC16CM_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3140932.1"
                     /translation="EALWKATLEGTGGVIARGDTAYANPVSAFDLDSGELLWKAEGEG
                     SGASGGPALSPSGEVLYAATEDEGGGSSVAAYDASGGEELWRAALGDELVDPSDRLWA
                     SGNVVLVPLLSDDIVALDAGTGEEVWRYTPPAPRLGNITVEGGEVWFALQNGEVLALD
                     AEGGQISARSNDYSLNLGSTSLSQRPVFVEGTLVLGVGTYVLGFQAPEGEGGP"
BASE COUNT          562 a          998 c          935 g          419 t
ORIGIN      
        1 acctcacgcc cgcgcgaccc tccgggccaa ggagaggcat aagcgacgcc catcatcctg
       61 cctgacgcct cccctcccgg ctttccggag cagaccggga cagaaaccct ccgtcgctcg
      121 atccgcgtgc tggacaggtg gccagaggac aacgaaggac gcctagggga cgaggtgcgc
      181 gttaggtgag tgatcgaggc gtgtaaccct gggagggcct gacgcaaaag cgtaacccga
      241 aattgtttgt actttggtgg atacgaaacc ctctcgtcga agtactgcgc gaggcaggtt
      301 aactgctcct tgctcagggt ttccaggtcg tctcccatga aagactccct ccgtggtctc
      361 gaagtgtggt cgggcgatag cgtacgcgca ctttccgaaa gtgccaagcc cgagggttcc
      421 tagggcgtgg cctgctgcgc ctcccgatcc ggctctcgcg cggggtcggc ttcggtttcc
      481 tccacgtccg ggcgagcgag aataacggcg aagatggcgt agatcagggg tttcccgaag
      541 atgccgaacc cgccgagggc cgcctgcccg gtgaaaaagt cgacctggag gatgtcgtgg
      601 gaggcaaccg cccagttcag gaccgcgatg ctgaacagta tgtagcccag gtacagccag
      661 atgcggctcg cgcggggcag tcccctggag ccttcgttgg cccagaaccc gatggtcagc
      721 gcgtcccaga tcaggccgat cgcgatcatt cccacccctg tgaaaccaag aacagggctg
      781 aacgggtcct cgataaaccc cgtctgacgc atgaggagcg tgatcaggac gagcagcacg
      841 agccgtctgg ctctgatctc ggtgagggtg cggcgtgcgg cccagtacac ggccacggag
      901 gcgaagatca cgacccacca gaagtcgacg ggctcgaggc cgctccaggc gaagaagctc
      961 aagggccttc ccgggttggt gagggatatc cagagcgcgt ttgccccgac tatgccgagg
     1021 tagagggcca gcgtgggcct gcccctgcgc gccaggaaca cggcggcgac gatcgcaacg
     1081 ccggcgacga gcgtctgcca gttggcgtcc tgcccgatca ggccctctgc cagggcgagt
     1141 atgccgccgg gatcacccgg tcccgcgagg ccgacgcgta cgagcagaag gccgaggaga
     1201 ccaccgatca ggacaacgac gatctcgatt ccctggagcc ccaggtagat ggctatgagc
     1261 ggtagggcga cccttttggc cacgtcgagc aacgtctcta cggtaacggg gtcccccccg
     1321 taccgcctga ccacccacca caccatcccc acggagaggg ggatcacgag cgcccccgcg
     1381 taggcggcgg cctggtcggc gagagagctc ttcccgatcc tgccggcggc ctccataacc
     1441 acccatacca gcctccaccc gagcagcaga gcgaggatcg cgtacatcac gcgtcggccg
     1501 gcccagatgt cggctaggcc agaggtccag ctcgccgtct tgtgcacgaa ctccgcgata
     1561 tccaccccga gatagagcat gagcggtatg accagcatgt cgaaggtgta gagcagggtc
     1621 ggcacgttgg cgaggacacc tgggacacca ctcgagcgcc aggcgtctat ggcgtagatc
     1681 tgggagaccc cgaaggtcag cgcaacgagg acgagtagca cgccgaactc gaacgccggc
     1741 gcctccgcgg ccttccgcct gaggaagaag aacacgggca ccacgagcaa cgcacccacc
     1801 gacaccagcg ccgcggggat gccggtgacc gtgctcgccg tccagacgct ggccacgacg
     1861 aggtagagca gcagcacggc cagcttgacg agaagcctgc tgtgcagggc cccggcgagc
     1921 atgaagcccc acgccaccga gaggatgaag atcgtaacgg ggatgaggac gaagggaacg
     1981 agctcccctc ggcccgtcgt gccgcctacc atgggtagca gggggaaggc ctcccgcaag
     2041 ggctcgttga agaacagcat gccggtgccg gtaaagacga ggatgaaacc gagccaggca
     2101 agggcccgcg cgggccccgg caggtcgccc acgtgcacca cgccgggccg caggtcgtcg
     2161 accaccagcc gcttcatcgc atcggagaac gtcccgaacg tcgcccggcc cgccgacttg
     2221 gccgcggagc gaacgggcgt gtgctgcgtg accttgatcc tcctcaaccg actcaaggtc
     2281 cgccctcacc ttcaggcgcc tggaaaccga gcacgtaggt cccgacccca agcaccagcg
     2341 tcccttccac gaacacggga cgctgggaca ggctcgtgct gcccaggttc aggctgtagt
     2401 cgttggagcg ggcggagatc tgaccaccct cggcatcgag ggcgaggacc tccccgttct
     2461 ggagggcgaa ccacacctct cccccctcta ccgtgatgtt cccgagccgc ggcgccggcg
     2521 gcgtgtaacg ccacacctcc tcaccggttc cggcgtcgag cgccactata tcgtcggaga
     2581 ggaggggcac gagcacgacg ttcccgctgg cccacaggcg gtcagagggg tcaacgagct
     2641 cgtcacccag cgcggcgcgc cagagctcct ctccacccga cgcgtcgtag gcggccaccg
     2701 atgatccccc gccctcatcc tccgttgccg cgtacagtac ctcccccgac gggctgagag
     2761 cgggcccacc gctggctccg ctcccctccc cctcagcctt ccagaggagt tctccggaat
     2821 cgaggtcgaa ggccgaaaca gggttggcgt aggctgtgtc gccccgcgcg atcacgcccc
     2881 ccgtaccctc cagagtagcc ttccatagcg cctc
//
LOCUS       DBNGVD010000507         2808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1404458, whole genome shotgun sequence.
ACCESSION   DBNGVD010000507 DBNGVD010000000
VERSION     DBNGVD010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1404458"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1712
                     /locus_tag="AC16CM_13695"
     CDS             <1..1712
                     /locus_tag="AC16CM_13695"
                     /inference="COORDINATES: protein motif:HMM:NF052715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3140933.1"
                     /translation="SAVARVCRRLDGMPLAIELAAARTRVLSAGQIADRLDESFRMLK
                     SNVRTSLPRQQTLRATMDWSFGLLYEEEKNLFARLSVFTDGFTLAAAEEVCGADGIDR
                     DEVADILFRLAEKSLLVVDRREEGEQTRFRLLETVREYASERLQENGEARLLGSRHAA
                     YFLRLAERAAPELFGPDQATWLGTLEAEHDNLRSALGWALGEGGEAGLRLANALGEFW
                     YMHGHLEEGRRWLDRGLASTGSHSPLLRARALEKVGWIAIFQNDDEATTLLEESLRLF
                     KELGDEQGSALALAGLGWALLYLAGDESRLLTLRAEAEVLRQGSLDRWTLSHLLTFLA
                     LAALVEGDHDHAVALSEESMALSRELGDTLGLAICTHTLGMAAAGQGDYERASALFEK
                     NLRVLWEQGDRVNVGHCLLGLGGVAISQGHPERATLLWGAAESLREATGSTLTPLVRS
                     HYAYEARLADARSRMGEAAFLEAWDRARALTPRQAVEYALEREDRDPRTYAARALPAG
                     LSEREAEVLALAARGLTSAQIGEKLFISPRTVNRHLDSVYKKVGVNSRAAAARFASEH
                     GIA"
     gene            1925..>2808
                     /locus_tag="AC16CM_13700"
     CDS             1925..>2808
                     /locus_tag="AC16CM_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3140934.1"
                     /translation="MYETSAEKKWPVSRVAALLAAVFFVLGGFLTLSVSPARASFPGA
                     TGKIVFQSERTVGPGVHNPEGDYEIFSVKPDGSGLVQLTDNNQPDLEPSYSAYGNRIA
                     FVRNSDIWVMRSDGMDQTRLTYNITQQSGYAHDPAFSPDGTRIAFSLTSTGFGSEVYT
                     MDSDGSHQKNLSSSPGSDYGPVFSPNGARIAFTSDRDGDDEIYRMRADGTGNPVNLTN
                     NDTNDTRPDFSPDGKRLAYTSYRMGSMELFTMRADGTDKVRLTRTAYEEGAPVYSPDG
                     KRLAYEAGGSIARIRADGT"
BASE COUNT          522 a          921 c          919 g          446 t
ORIGIN      
        1 catccgcggt cgcgcgggtt tgccgccgtc tcgacggcat gccgctggcc atagagcttg
       61 cagcagccag gaccagggtg ctctcggcgg gccagatcgc cgatcgcctg gacgagagct
      121 ttcggatgct caagagcaac gtccgtacct cgctgccccg gcaacagacc ctgcgggcga
      181 ccatggactg gagcttcggg ctcctgtacg aggaggagaa gaacctgttc gccaggctct
      241 ctgtcttcac cgacggcttc accctggccg cggccgagga ggtgtgcggg gcggacggta
      301 tcgacaggga cgaagtcgca gacatcctct ttcgcctggc cgaaaagtcg ctgctcgtcg
      361 tggatcgacg cgaggaaggc gagcagacac gatttcgtct gctcgagacg gtaagggagt
      421 acgcgtccga gcggctccag gagaacggcg aggctcgcct cctgggaagc aggcatgctg
      481 cctactttct ccgccttgca gagcgcgccg cccccgagct atttggcccc gaccaggcca
      541 cctggctggg gacgctcgag gccgagcacg acaaccttcg ctcggccctt gggtgggcgc
      601 tcggcgaagg cggcgaggcc gggttgaggc tggccaacgc cctgggagag ttctggtaca
      661 tgcacggcca cctcgaggaa ggacgccggt ggctcgaccg cgggcttgcc agcacaggct
      721 cacactcgcc acttctacga gcacgggcgc tagagaaggt gggttggatc gccatattcc
      781 agaacgacga tgaggcgacg acgctgctcg aagagagcct gcgcctgttc aaagagctgg
      841 gcgacgaaca gggcagcgcg ctcgcgctgg cggggctggg ttgggcgctg ctgtatctgg
      901 caggggatga aagccgcctt ctgaccctcc gcgcggaggc cgaggtattg cgacaggggt
      961 ctctggaccg gtggacgctc tcgcacctgc tcaccttcct ggccctggcc gcgttggtcg
     1021 agggagatca cgaccacgcc gtcgcactct ccgaggagag catggctctc tcgcgggagc
     1081 tcggcgatac gctgggcctg gccatctgca cgcacaccct gggcatggcc gccgccgggc
     1141 agggagatta cgagcgggct tctgccctgt tcgagaagaa cctgcgcgtg ctgtgggagc
     1201 agggcgacag ggtcaacgtg ggccactgcc tgctgggcct aggcggggtc gcgatctcgc
     1261 aagggcatcc ggagcgggcc accctgttgt ggggggctgc cgagtcactg cgggaggcga
     1321 ccggttctac cctgaccccc ctggtcaggt cacactatgc ctacgaggcg cgcctggcag
     1381 acgcccgctc gcgcatgggt gaggccgcgt ttctggaggc gtgggacaga gcgagagcct
     1441 tgacgccgcg ccaggccgta gaatacgcgc tcgaacgaga ggaccgggac ccgaggacgt
     1501 acgccgcccg tgccttgccg gcgggtctga gcgagcgtga ggccgaggtg ctcgcgctcg
     1561 ccgcgcgggg cctcaccagc gcccagatcg gcgaaaagct gttcataagc ccgcgcaccg
     1621 taaaccgcca tctggactcg gtgtacaaga aggttggcgt caactccagg gccgccgctg
     1681 cccgcttcgc ctccgaacac ggtatcgcct aacccttccg ccgcacttct ccgctcctca
     1741 taagggacgc gccgcgtccc gtgttccgcg aacgatcgcc tggtagtggg gtacccacgt
     1801 gttctcgacc aggaatgggc acccccgatg ggtagttctg acgatgccgt ctcctcccgg
     1861 ttctcccatt atgtcgtcac gttatcgacg cccgtcagga gaggcggggg agaggagcaa
     1921 gaacatgtac gagacgagcg cagagaagaa gtggccggtc agcagggtag cggcgctcct
     1981 ggcggctgta ttcttcgtac tcggcggatt tctgactctg tcggtgagcc cggcccgggc
     2041 aagcttcccc ggggccaccg gcaagatcgt ttttcagagc gaaaggacag tcggaccggg
     2101 cgtgcacaac ccggaaggag actacgagat cttctccgta aaacccgacg gctcgggtct
     2161 cgtccagcta accgataaca accagcccga tctcgaaccg tcctactccg cctacggcaa
     2221 caggatagcc ttcgtgcgaa actcggatat ctgggtgatg aggtcagacg gcatggacca
     2281 gaccaggctc acctacaaca taacccagca gagcggctac gcccacgacc ctgccttctc
     2341 gcccgacggc accaggatag cgttcagcct cacctccacc ggcttcggct ccgaggtcta
     2401 caccatggac tccgacggct cgcaccagaa gaacctctcc agcagccccg gctcagacta
     2461 cggtcccgtc ttctcgccca acggtgcgag gatagccttc accagcgacc gcgacggaga
     2521 cgacgagatc tacaggatga gggccgacgg taccggaaac ccggtcaacc tcaccaacaa
     2581 cgataccaac gacacacgac cggacttctc ccccgacggc aaacgtctag cctacaccag
     2641 ctaccgtatg ggtagtatgg agctcttcac catgcgggcc gacggcaccg acaaggtccg
     2701 tttgaccagg accgcctacg aggaaggcgc accagtctac tcgccggacg gcaagaggct
     2761 ggcatacgag gcgggcggct ccatcgccag gatcagggcc gacggcac
//
LOCUS       DBNGVD010000508         5533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1768932, whole genome shotgun sequence.
ACCESSION   DBNGVD010000508 DBNGVD010000000
VERSION     DBNGVD010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1768932"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16CM_13705"
     CDS             complement(<1..505)
                     /locus_tag="AC16CM_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR3140935.1"
                     /translation="MRLLGIVALVFLFAGLTACSSESRDGGRDGDGGDGAGTEHQATT
                     ETTEREAETSSPEDADIWNYVALGDSLAEGVGARRGYVDMYADHLREETGARVELDNL
                     GVSGQTSSQLLHAVRNDPSMRRALRGANVVTYNIGINDLGQAWGFYDAETCGGAQGER
                     CLHAAVGD"
     gene            complement(641..1897)
                     /locus_tag="AC16CM_13710"
     CDS             complement(641..1897)
                     /locus_tag="AC16CM_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HIR3140936.1"
                     /translation="MTIGKPKSSNTASWRPRLRGFIFVVGILLLAANLRPALTGVAPL
                     IGEIRADTGISNGAAGLLTTLPLLAFALLSPVAPRVARRFGLEAVLLASLLVLAAGIL
                     FRSVGTVATLFIGTAILGSAIAFGNVLLPGLVKREFPEKAGLMTSVYSTSLGISAAIA
                     AGVSVPLTGLDGIGWRGALAVWALPAFLACVAWLPQLGRRDLPARSSDPNAPIIRDLL
                     RSSLAWQVTLFMGLQSLSYYVTLTWLPEILQDGGMDAANAGWMLGLSQTVSILAMFLA
                     PVLAVRRPSQWGVVVVAVILSSAGALGLLLAESAAPALWVVLLGLGQGASFSLALAFF
                     PLRSPDPGHAAALSGMAQSVGYLLAASGPFLFGVLRDATGAWNVPLVLLLAITVCLLF
                     AGLGASRDAHVRSSNREDPVEDSSNE"
     gene            complement(2048..2929)
                     /locus_tag="AC16CM_13715"
     CDS             complement(2048..2929)
                     /locus_tag="AC16CM_13715"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3140937.1"
                     /translation="MQVHRDVLIIAVPYDSGNPDLRMGAGPQHLLDNGLGASLRPDGR
                     GVSVITVRHESEPPAEVSTAFELQSLVSRQVRAAAGEGRLPLVLSGNCNTAAVGSLAG
                     LGPWDFGIVWFDAHGEFNTPETTTTGFLDGMGLAMAVGHCWKEMARGVPGFVPIPEEN
                     VVMAGVRAADVAELERFDASGVTVVGPDAGEQQMQALAEALDDLQSRVGRVYVHLDLD
                     VLDAAEVGMANEYGIPGGLDRGRLEEALDLVRERFTVAAAGIASYDPSFDADGSVLDA
                     AIAGARLLTSPKTGEVG"
     gene            complement(2965..3678)
                     /locus_tag="AC16CM_13720"
     CDS             complement(2965..3678)
                     /locus_tag="AC16CM_13720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR3140938.1"
                     /translation="MEDRMKLLLTSVGATNDSIRGALVELLGRPVEDCRAVQINTALY
                     ASPHGPEDAYEMAKHYGAMGWGELGTLELTALPTIEEEHWLPFVESCDAILVSGGNTG
                     YLSFWMQESGFAEKLPGLLRSAVYFGISAGSTMLTPGQNYDPERLENEGIYYDDEFEE
                     PAPRGAGSAWGMGLVDFSIRPHLGADYFPVAVDMAMMERAASRLDHPMYAIDDETAIK
                     VVDGKVEVVSEGEWRYFER"
     gene            complement(3683..4240)
                     /locus_tag="AC16CM_13725"
     CDS             complement(3683..4240)
                     /locus_tag="AC16CM_13725"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3140939.1"
                     /translation="MGKVVASTNIALDGVVEAPDRWFFPFWSEELSRSAHEELLASDA
                     LLLGRVTYEEFAEFWPTATDESEIAEQMNSLPKYVASTTLTEPLSWNPTTLFEDHVPE
                     EVAWLKKQTGGDILLLASASLMQTLMEHDLVDEYRLRVAPAVAGGGKRLFSEGSGTRN
                     LSLVETRTFDTGVVALTYRATGNGG"
     gene            complement(4268..4765)
                     /locus_tag="AC16CM_13730"
     CDS             complement(4268..4765)
                     /locus_tag="AC16CM_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3140940.1"
                     /translation="MAAGGDPRPGELVVTRDFDAPRELVFRAWTEPERVRRWWGPEGF
                     TMPYCDIDLRPGGTFLRCMRAPGGLDFWVTGVFREIVAPELLVFTDSFADKEGNVVPP
                     TRYGAGSGMPLERLVTVTFEELAGSTRVTVRHAGLPSGEDRDLVRHGWAGGLESLSGY
                     LSGAS"
     gene            complement(4749..5096)
                     /locus_tag="AC16CM_13735"
     CDS             complement(4749..5096)
                     /locus_tag="AC16CM_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3140941.1"
                     /translation="MSSSTLDSTFAALADPTRREILVRLASGEASVKELAEPFQMSLP
                     GVSKHLKVLERAGLISRGRVAQSRPCRLEARPLKEAADWVEHYRRFWEEGLDRLEDYL
                     QEIQREGEGGGGR"
     gene            complement(5267..>5533)
                     /locus_tag="AC16CM_13740"
     CDS             complement(5267..>5533)
                     /locus_tag="AC16CM_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140942.1"
                     /translation="FQTMGEIAVNQNSTIILPVPIDLLRPFMEATNGTDGNSSYGQEV
                     KAARRKEEEEAEAERLYEEAVGEARSEVSSEEVPAEMPDGEGGR"
BASE COUNT          974 a         1878 c         1747 g          934 t
ORIGIN      
        1 gatccccaac ggccgcgtgc aggcaacgct cgccttgcgc gcccccgcag gtttcggcgt
       61 cgtagaagcc ccaggcctgt cccagatcgt tgatgccgat attgtaagtg accacattgg
      121 cgccgcggag cgccctccgc atagaagggt cgttcctgac ggcgtgcaaa agttgcgagc
      181 tcgtctggcc gctcaccccc aggttgtcga gctcgacgcg ggcgcccgtc tcttcgcgga
      241 gatggtccgc atacatgtcc acgtagcccc gccgcgcgcc caccccctcg gcaagcgaat
      301 cgccaagggc cacgtagttc cagatatcgg cgtcttcagg agaactcgtc tcagcctcac
      361 gttctgtcgt ctccgtcgtt gcctggtgct ctgtgcccgc tccgtccccc ccatcgccgt
      421 cccgcccacc gtctctgctc tccgaagagc aggccgtcag gccggcgaac aggaacacca
      481 gagcgacgat tccgagcagc ctcaccggtg gcttcccggc cacgtaagcc cgcgccgggc
      541 tgtcgggcga acgcgtcggt tcttctgtgc gtgcagcatt tcctcattct accgttcgat
      601 tggtgtggac catcgtcctc atgcccggga ggcttcctgg ttactcgttg ctgctgtcct
      661 cgaccgggtc ttcgcggttg gatgagcgga cgtgggcgtc ccgggaggct cccaggcctg
      721 cgaacaggag gcacaccgtg atcgccagca gcaacaccag cggcacgttc cacgcgcccg
      781 tcgcgtcgcg cagcacgccg aaaaggaaag gtcccgaggc ggccagcagg taaccgaccg
      841 actgggccat gcccgagagc gcggcggcgt ggccggggtc tggggagcga agcgggaaga
      901 aggcgagggc caggctgaat gaggctccct gcccgaggcc gaggagcacg acccagagag
      961 caggagcggc ggactccgca agcagcaaac ccagagcgcc cgcggacgac aggatcaccg
     1021 ccaccaccac gacgccccac tgggaaggtc tcctcacagc cagcacgggg gccaggaaca
     1081 tcgccaggat cgacacggtc tgggagaggc caagcatcca ccccgcgttg gccgcgtcca
     1141 tgcccccgtc ctgcaggatc tcggggagcc acgtcagcgt cacgtagtag gagagcgact
     1201 gcagccccat aaacaaggtt acctgccagg ccaacgagga gcgtaacagg tccctgataa
     1261 tgggggcgtt gggatcggac gatcgcgcgg gcagatccct gcgtccgagc tggggcaacc
     1321 aggcgacgca ggcgaggaac gcgggcaggg cccacaccgc aagtgcccca cgccagccga
     1381 tgccgtccaa cccggtcagg ggaacgctca cgccagcggc gatcgcggcg ctgatgccca
     1441 gggaggtgga gtacacgctg gtcatcaggc cagccttttc ggggaactcc cgcttgacga
     1501 ggccgggcaa gagcacgttg ccgaacgcta tcgccgaccc aaggatggcc gtgccgatga
     1561 agagcgtggc gacggtaccc accgaccgaa acaggatgcc ggccgcgaga acgagcaggc
     1621 ttgccagcaa cacggcttcc aggccgaacc ggcgggccac gcgtggagcg acgggcgaga
     1681 gcagggcgaa cgccagcagc ggtagtgtcg taaggagacc cgcggccccg ttggaaatgc
     1741 ccgtgtccgc tcttatctcc ccgatcaggg gcgccacacc cgtgagcgcc ggccgcaggt
     1801 tggccgcgag gagcaggatg cccaccacga agataaagcc acgcaggcgc ggacgccagg
     1861 acgccgtgtt ggaagatttc ggtttgccta tagtcactcc gccagatgct accccgcaga
     1921 agagcggaac aaacgccagg cgcggacgtc gtgaagagcg ggccgattat tggggaaggt
     1981 tacaggcatc aggcttcagt agatttacct gtagcctatt tccggaccat cgttgccggg
     2041 agctgcacta accgacctcg ccggtcttcg ggctggtcag caggcgcgcg ccggcgatgg
     2101 ccgcgtcgag tacgcttccg tcggcgtcga aggatgggtc gtaggaggct ataccggcag
     2161 cggcgacggt gaagcgctcg cgcaccaggt cgagggcctc ttctagtcga cctctgtcca
     2221 atcctcccgg gatgccgtac tcattggcca tcccgacctc ggcggcgtcc aggacgtcca
     2281 ggtcgaggtg cacgtagact ctccccaccc gggattggag atcatccaac gcttctgcga
     2341 gggcttgcat ttgctgttcc ccggcgtctg gtccgacgac ggttaccccg gaagcgtcga
     2401 accgttctag ctccgctacg tcggcagccc tgacccccgc catgacgacg ttctcttctg
     2461 ggatcgggac gaagccaggc acgccccttg ccatctcctt ccagcagtgg ccgacggcca
     2521 tcgcgaggcc catgccgtct aggaagccgg tggtggtcgt ctcgggcgtg ttgaactccc
     2581 cgtgcgcgtc gaaccagacg atgccaaagt cccaggggcc gagaccggcg agggatccga
     2641 cggccgctgt gttgcagttg ccggagagaa ccagcggcaa ccggccctcg ccagcggcgg
     2701 cgcgtacctg ccgcgagacc aggctctgca gttcgaaggc ggtcgacacc tcggccggcg
     2761 gctcgctctc gtggcgcacg gtgataacgc tcaccccacg tccgtcgggc cgcaggctgg
     2821 cacctagccc gttgtccagg aggtgctggg ggccagcgcc catcctcagg tcggggtttc
     2881 ccgagtcgta gggcacggct atgatcagaa cgtcccgatg gacctgcacg atggtctcct
     2941 atctctggcg ctgactcggc accgctagcg ttcgaagtac ctccactcgc cttcggagac
     3001 cacctcgacc ttcccgtcca cgaccttgat cgccgtctcg tcatctatag cgtacatcgg
     3061 gtgatccaac ctcgaagccg cccgctccat catggccata tctacggcga cggggaaata
     3121 gtccgcgccg aggtggggcc gtatggagaa atccacgagg cccatacccc aggcgctgcc
     3181 ggcgccacgc ggcgcgggct cctcgaactc gtcgtcgtag tagatacctt cgttctccag
     3241 ccgctccggg tcgtagttct gaccgggcgt gagcatggtg ctgccggcgc taataccgaa
     3301 gtacaccgcg ctcctgagca aaccgggtag cttctcggcg aaaccggact cctgcatcca
     3361 gaagctcagg tacccggtgt tgcccccgct gacgaggatg gcgtcgcagg actcgacgaa
     3421 cgggagccag tgctcctcct cgatggttgg cagggccgtg agctccaggg tgccaagctc
     3481 gccccagccc atcgcgccgt aatgcttcgc catctcgtag gcatcttcgg ggccgtgggg
     3541 agaggcgtag agggcggtgt tgatctggac cgcccgacaa tcctctacgg gcctgccgag
     3601 cagctccacg agcgccccgc gtatcgagtc gttcgtggcg ccgacggacg tgagtagaag
     3661 cttcatacgg tcctccatgg ggtcatcctc cgtttccagt cgcccgatac gtgagggcga
     3721 cgacgccagt atcgaacgtc ctggtctcca ccaggctcag gtttctcgtg ccgctgcctt
     3781 cggagaagag acgtttgccg ccgccggcca ccgcgggggc gacgcgcagg cgatactcgt
     3841 ccacgaggtc gtgctccatc aacgtctgca tcaggctcgc gctggcgagc aacaggatat
     3901 ccccgccggt ctgcttcttg agccaggcaa cctcctctgg cacgtggtcc tcgaaaagcg
     3961 tggtgggatt ccacgacagg ggctccgtga gggttgtcga ggcgacgtac ttgggcaggg
     4021 agttcatctg ctcggcgatc tccgactcgt ccgtggcggt gggccagaac tcggcaaact
     4081 cctcgtaggt caccctcccg agaagcaggg cgtcgcttgc gagcagctct tcgtgggctg
     4141 acctggagag ctcctcgctc cagaaaggaa aaaaccatct gtccggcgcc tccacgacgc
     4201 cgtccagcgc aatgttcgtg gacgctacta ccttccccac cccatcctcc ttcgacgtcg
     4261 atgttctcta cgatgcccct gacaggtaac cggagaggct ctccagaccg cccgcccagc
     4321 cgtgccggac gagatcccgg tcctcccccg acgggagacc ggcgtgcctg acagtgaccc
     4381 tcgtagaccc cgccagctcc tcgaacgtaa ccgtgaccag cctctcaagc ggcatgcctg
     4441 accccgcccc gtagcgcgtg ggagggacca cgttgccctc cttatccgcg aaggagtcgg
     4501 tgaagacaag cagctccggc gcgacgatct cgcggaacac acccgtcacc cagaagtcca
     4561 ggccaccggg cgcgcgcatg cagcggagga aggtgccgcc cggacggagg tcgatgtcgc
     4621 agtacggcat cgtgaagccc tctggacccc accagcgcct cacacgctct ggctccgtcc
     4681 acgcccggaa tacgagctcg cgcggcgcgt cgaagtcgcg cgttacgacg agctccccgg
     4741 ggcggggatc accgcccgcc gccaccctcc ccctctcttt gtatctcctg caggtagtct
     4801 tccaggcggt cgaggccctc ttcccagaaa cgacggtaat gctccaccca gtctgcggcc
     4861 tccttcaggg gccgcgcctc tagccggcaa ggccgcgact gcgccacgcg gccgcgcgag
     4921 atcaaaccgg cccgctcgag caccttcagg tgcttggaga cgccggggag gctcatctgg
     4981 aatggctcgg ccaactcctt gacggaagcc tcaccggagg ccaaccgaac cagaatctcc
     5041 cgccgggtag ggtccgcaag ggccgcaaac gtactgtcaa gcgtgctcga tgacatctta
     5101 tataaccttt cggttaatta actaactagc aaaatacaac cgaaaggaac gtgtgtcaag
     5161 cggcgcgaca ggtgggtcca gtgggttccg cgtgggaaga gcgcaacgat tgccgggtag
     5221 ggccggtgta cttccctggc tttgttctat ttcgtggttg gcggtgtcaa cggccgcctt
     5281 cgccgtcggg catctcggcg ggcacctctt cgctggacac ctcgctccgg gcttctccta
     5341 cggcctcttc gtagaggcgc tctgcctctg cctcttcctc ctccttgcgc cgggccgcct
     5401 tgacttcctg tccgtaggat gagttcccat ctgtgccgtt ggttgcctcc ataaagggtc
     5461 ggagcaagtc tatagggacc ggcaggatga tggtggagtt ctggttgacg gcgatctcgc
     5521 ccatcgtctg gaa
//
LOCUS       DBNGVD010000509        13735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4500857, whole genome shotgun sequence.
ACCESSION   DBNGVD010000509 DBNGVD010000000
VERSION     DBNGVD010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4500857"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1893)
                     /gene="glmS"
                     /locus_tag="AC16CM_13745"
     CDS             complement(58..1893)
                     /gene="glmS"
                     /locus_tag="AC16CM_13745"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3140943.1"
                     /translation="MCGIVGYAGRDRCVEVLMESLGYLEYRGYDSAGLALQSEGRIER
                     IREVGRLENLARTIARRNGELSPLRTGVGHTRWATHGSPTEENAHPQLGGNGTVAVVH
                     NGIIENFAELKEELQGRGFRFRSETDTEVMAHLIAERVDGGESLREALAGVLRQLEGT
                     FAVAALSAGEPGKVVAARHQSPLVVGFGEGENFLASAVQALLGETSQFLHVENGEIVE
                     VTDSSVEVFTPEGEHVERESFEVDWEAEAVELGGYRDYMLKEIHEQPAALGATLLGRI
                     DVDGAVDLSEIDLDFTNTDRVVVVACGTAYHAGLLGKHAIEKLARIPVEVEIASEYRY
                     ADPIGNESTLVVAISQSGETTDTLAAIQEARRFGAKVLAVTNTRGSLITREADAVLLT
                     KAGPEICVASTKAFACQVAVLDLLALQLARARGVMPEEALREAGRGLRLAPEKVEETI
                     QGLGERVGEAAAVFEAARCSLFLGRGISYPVALEGALKLKEISYLPSEGYPSGEMKHG
                     PIALVDEYCPVVAILGDGVVREKTISNVEETLARGANVIAVAREGDRVAQRIAGVLLP
                     VPEVPELVSPLVASVPLQLLAHRVATDRGLDVDKPRNLAKSVTVE"
     gene            complement(2267..3055)
                     /locus_tag="AC16CM_13750"
     CDS             complement(2267..3055)
                     /locus_tag="AC16CM_13750"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HIR3140944.1"
                     /translation="MARTAEERGTKRGAAQKVARFWEVDAARGVAIIMMVVYHTTYDL
                     DVLGGYDIDATSGSWAVFADVTAGLFLFLVGVSLAISRSRTSLSGWRLFGKYLARGLR
                     ILAYGMILTGIFLALQMGVVAFGILHLIGVSVILAYPFLKLRVTNLVLGLIVFVAGQY
                     MLAQDYISETYWLLPFGVVPEGLIMPDYRPLLPWFGVVLIGLYFGNVVYADGHRPAGL
                     PDRAPLLGRPLLPLGRNSLFIYLIHQPIVIALLALTGIISLNIL"
     gene            3206..3682
                     /locus_tag="AC16CM_13755"
     CDS             3206..3682
                     /locus_tag="AC16CM_13755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140945.1"
                     /translation="MEADFTIRPGRKEDAPEAARLWMQSADEHTAHDRVYETSPDAER
                     TMRRFLADVANSGYSFLFVAVTEDRTVGFISGELRQGSPTFLPKTWASVDDVFVEPEH
                     RNRGMGRALLRSVQAWARERDADGISLQVAAANARGRKFYGELGFREVSVYELLEF"
     gene            complement(3695..4153)
                     /locus_tag="AC16CM_13760"
     CDS             complement(3695..4153)
                     /locus_tag="AC16CM_13760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3140946.1"
                     /translation="MSPIEVTPAPDLAEPLSLLETSLRDGEPVPEAFIESLREHVRTG
                     TMDVLAARSEGRTLGVAVLAYRPSIAAGALFASIEDLYVRPEARRQGVGRALLKAADK
                     RCKAHRISYLEVQVEDKTAESFYAAVGYEPEETVRVLSRSLPIDVPNIKG"
     gene            complement(4150..5169)
                     /gene="ltaE"
                     /locus_tag="AC16CM_13765"
     CDS             complement(4150..5169)
                     /gene="ltaE"
                     /locus_tag="AC16CM_13765"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR3140947.1"
                     /translation="MIDLRSDTVTRPTEAMRRAMLEAPVGDDVFGEDPTVNRLEEYVA
                     DLLGKEAALYAPSGTMTNQIGVQVHTNRGEEVLLHEGSHIFVYEAGAPAMLSGVQLRT
                     LPGDNGILDPDTVRAAVRSEDVHFPRSRLLCLENTHNTAGGKVFPLQDFAAVAETARE
                     LGLKVHLDGARLFNAQAATGIPAREWCAHADTVSVCSSKGLGAPVGSLLAGDGETIRE
                     ARRARKAFGGGMRQAGIIAAASIYAFENNTGRLSEDHERARNLAAGLREAGYEAREPD
                     TNLVLVAVDDPPEFLRALATEGVLATLGKPGFVRLCTNLDVGDEDIETAIEAAARILA
                     TTSGR"
     gene            complement(5166..6110)
                     /locus_tag="AC16CM_13770"
     CDS             complement(5166..6110)
                     /locus_tag="AC16CM_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013514.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140948.1"
                     /translation="MNGSGTDLPDGLRLLLAREGVSVTSSRPIDHGTQYDLARAGETA
                     KLNVYHTGKVSAGGKASGLRDLLDGWRTSQRGAPATRNPTRTSSRSSGSMPVLDRTPR
                     LGIDEAGKGDYFGPLVVAGVRITGEAQAKSLQEIGVRDSKEIGVLGVGRISERILEAV
                     GPENVRVVSLSPREYEVRRGAAGNINGLLGELDAGIVVELAGEVEVVVVDEFARSARD
                     HLRPFVPRGVRLEVRPRAEDDAAVAAASIVARARQLEEIERLSEQVGFRLPLGATHVV
                     DAGRRVVEELGEEGLAGVAKVHFATTRRVLARHDGGRP"
     gene            complement(6107..6391)
                     /locus_tag="AC16CM_13775"
     CDS             complement(6107..6391)
                     /locus_tag="AC16CM_13775"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3140949.1"
                     /translation="MQGKDLERAYVRISGRVQGVFFRDATRQKAEDLTLAGWVRNLPD
                     GRVEAVFEGHPDRVKEMLRWCEEGPRQASVENVDTDIEDAAGDLSGFEVR"
     gene            6457..8013
                     /locus_tag="AC16CM_13780"
     CDS             6457..8013
                     /locus_tag="AC16CM_13780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3140950.1"
                     /translation="MKAQRETVGYGTEGGEGSSRVLLTLVIPTRNEVDNVPELMAGLR
                     ESLMAVDYRVVFVDDSTDGTPTVIKDLAREDARVSLVRREGSERRGGLSTAVTRGLDL
                     SSEVSEYTCVMDADLQHPPEKVREMLETARHTDADVVVASRYAEGGSYTGLSGTMRRA
                     VSVGSKYLARIVFKEARKTSDPMTGFFLLKNEAISGIQFRPTGFKVLLEILVCAPELK
                     VVEEPFDFRARHAGVSKATLRQGLDYLIHTLSLFWYVPAAGRFWKFCLVGASGVLVNM
                     VTLIVLAEVFDAHKVIAWMFAVGLSILSNFLLNNAFTWRDVRHSSRIHFFLRGVLAYP
                     VAVMGIGANFAVWYPLIKYVSDAFPYYAIFNLLGIIAGTSVNFILSSRLVFRPSEQKS
                     LNPDAPRGQITEGIRRELRADRVALIPVSGLTPLGTYDHQELTVTDRGVIDLVIKTSH
                     PTLTVTGPRRLPQARTNARHSNSLAVPIFDEERTVGVVYATRNSTEPFTEEDLHWLTA
                     YTSTASELFT"
     gene            8417..9322
                     /locus_tag="AC16CM_13785"
     CDS             8417..9322
                     /locus_tag="AC16CM_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3140951.1"
                     /translation="MYSLGFGESGEMGLVEKLGAEGVGAFYTTGMRPVCFLSDFGLAD
                     DFVGTCKGVIHRIAPGVSVVDLTHAVPDFGVETGAELLEHATRYMPADAVYLAVVDPG
                     VGTERRGIALSTGGGAMMVGPDNGLLIAAAESLGGISEVVTLTKERFHLRPISNTFHG
                     RDIFAPVAAHLVAGVGLQEFGESVDPASLVRLETGSFPSERGGGMTTRILSLDHFGNA
                     RLSATTEQAGLEYGDALKVDAGDGGMSVRYVETFGSARAGELVLVPDSHWRLSLAINK
                     GNAGQALGLRVGGTVRLIPAESDDG"
     gene            9396..10091
                     /locus_tag="AC16CM_13790"
     CDS             9396..10091
                     /locus_tag="AC16CM_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140952.1"
                     /translation="MLRSAALRPINVMVLVIGVVMSVITAVWWLLPLTFVTYASLVFL
                     SARDPIFQRKVLGGGRSAALPPTTRDIEVSPERRARWLPRGETRRKVEETLQVYRKVV
                     AAIEASDDVTRAVLDDAVPRLHAAADRLVDVAEAREKANEVAGELRQDPGATADSTRQ
                     ENLHHLEEQIIAADAEISGTSEELVNLRAKVVRLSIDANNPTRARDLNTSLDSLNTRL
                     EALSETMSPREEG"
     gene            complement(10373..10912)
                     /locus_tag="AC16CM_13795"
     CDS             complement(10373..10912)
                     /locus_tag="AC16CM_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140953.1"
                     /translation="MREDMQYISGVLDELEAIVQDASGVPMSKGRAVVDRSDLLVMLD
                     ELRGSLPPELSEAEALRRERGATIAAAEEEAQRIVEEAHHRANAMVPETEIVSRAERR
                     AGEIIDGADRYAEEVSGGSEVYRDRVMEQLENWFQDSLISVEESRQELGGVPVRRSTR
                     EPEIVEEKPEDRGWHASSA"
     gene            complement(11284..11679)
                     /locus_tag="AC16CM_13800"
     CDS             complement(11284..11679)
                     /locus_tag="AC16CM_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140954.1"
                     /translation="MVATDVGIKCPDDARLPRGARAGVMKTNQLLRSVLAGLAVAVAG
                     VPVVWVLFQLPFTLLLSVAAGHGAGTLINRAGGRNGGPIAIAISVFATLFPFLVLMAP
                     NLLAGSINPYQLIAPLVAAIAAGVANRRI"
     gene            complement(11766..12170)
                     /locus_tag="AC16CM_13805"
     CDS             complement(11766..12170)
                     /locus_tag="AC16CM_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3140955.1"
                     /translation="MSIRRRPVYMIGVASELIGVHPQTLRMYEQKGLLRPRKSIKNTR
                     LYSQEDVELGRYIQMLTQELGMNLAGVSKVLDLERQVATLEKENRALREELERKELEH
                     HRLVAEVHQSYKRELVLMPRSELARSARKGRK"
     gene            complement(12167..13276)
                     /gene="dnaJ"
                     /locus_tag="AC16CM_13810"
     CDS             complement(12167..13276)
                     /gene="dnaJ"
                     /locus_tag="AC16CM_13810"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3140956.1"
                     /translation="MEKLDLYKVLEVSREASQDEIRRSYRRLARKYHPDANPGDKKAE
                     DRFKEIQHAYEILSKPEKRREYDEGPRTFFGSGETARPGNANFGEFSDLLGGFGNLGD
                     IFGRTTTTQRPQAKGENITINVRLSFKDALSGVTTRVGVPVEEACGECGGTGAAPGTS
                     PRTCPECGGRGVQSRDQGFFALSTPCSGCGGDGTIIEKPCAGCGGSGRVRRSKQVKVR
                     IPAGARDKMKVRIPGRGHAGRDGGPPGDLYVRTLVEEHPVFKRKADDFVVGVPVSFVE
                     AALGAEIQVPRPGGGTVRLKLTAGTQDGKQFKVRGAGAPRAKTNGGEKGDLIVRVNVV
                     VPKKVKRREREILEAFAEERDEDVREDLFKEVGSS"
     gene            complement(13280..>13735)
                     /locus_tag="AC16CM_13815"
     CDS             complement(13280..>13735)
                     /locus_tag="AC16CM_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3140957.1"
                     /translation="DEVARLTEELEATRLERDQYLDSLRRLKAEFENSRKRQERERSR
                     ILSMASERLVQELLPVLDNLDRALEAGGDIREGVQATREQLAEVLAEEGLLPVVSDGE
                     PFDPNIHDAVMGQPSEEHEDGTIIQTFQRGYLLNGKPIRPAKVVVAKQV"
BASE COUNT         2378 a         4562 c         4202 g         2593 t
ORIGIN      
        1 ctcggccggc acctttcgag gtgggagttt gagggggatt cgcgaagcat ccctacatta
       61 ttcgaccgtt acgctcttgg cgaggttgcg gggtttgtcc acgtccaggc cgcggtcggt
      121 ggccacacga tgggcgagga gttggagcgg gacgctggcc accagggggg atacgagctc
      181 aggtacctcg ggcacgggca gcagcacccc tgcaatgcgc tgggcgaccc tgtcgccctc
      241 acgggcgacc gcgatcacgt tcgcgcccct ggcgagggtt tcctcgacgt tggagatggt
      301 cttctcccga acaacaccgt ccccgaggat agccacgacg gggcagtatt cgtcgaccag
      361 ggctatggga ccgtgcttca tctcgccgga agggtaaccc tcggagggaa ggtaggagat
      421 ctccttgagc ttcaaggccc cttcgagcgc caccgggtag gagatgccgc gccccaggaa
      481 cagcgagcac cgcgcagctt cgaacacggc ggcggcctcc cccactcgct cacccagccc
      541 ctgaatagtc tcctccacct tctcgggtgc cagcctgagg ccacggcccg cctcccgaag
      601 cgcttcttcc ggcatgacgc cgcgagctcg ggcgagttgt agcgccagca agtcgagtac
      661 ggcgacctgg caggcgaagg ccttggtgct cgctacgcag atctcaggcc ccgccttggt
      721 gagcagtacg gcgtcggcct cgcgtgtgat gagagacccc ctggtgttgg tgaccgcgag
      781 caccttcgcc ccgaaccttc tggcctcctg gatggcggcg agggtatccg tggtttcccc
      841 gctctggctt atggcgacga cgagcgtgct ctcgttcccg atggggtccg cgtagcgata
      901 ctcgctggcg atctcgacct cgacgggtat cctggcgagc ttctctatgg cgtgcttgcc
      961 gagcaacccg gcatgatagg cggtgccgca ggcgaccacg acaacccggt ccgtattcgt
     1021 gaagtcgaga tctatctcgg agagatccac ggccccgtcg acatcgatac gtccgaggag
     1081 ggtcgcaccg agggcggcgg gctgctcgtg gatctccttg agcatgtagt ccctgtagcc
     1141 gcccagctcc acggcttctg cttcccagtc aacctcgaag ctctcgcgct ccacgtgctc
     1201 gccctctggt gtgaagacct ccacggagga atcggtgacc tcgacgatct caccgttctc
     1261 cacgtggagg aactggctgg tctccccgag cagcgcctga accgcgctgg cgagaaagtt
     1321 ctccccttcc ccgaacccga cgacgagcgg gctctggtgc ctggcggcga ccaccttgcc
     1381 aggctccccc gcgctcaggg ccgcgaccgc gaaggtgcct tcgagctgcc gaagcacccc
     1441 cgccagagcc tccctcaagg actccccgcc atcgacccgc tcggcgatga ggtgggccat
     1501 cacctcggtg tccgtctcgg accggaatcg gaagccgcgc ccctgcaact cctccttgag
     1561 ttccgcgaag ttctcgatga tgccgttgtg cacgacggcg acggtcccat tccccccgag
     1621 ctggggatgg gcgttctctt cggtgggact gccgtgggtg gcccagcggg tgtgcccaac
     1681 cccggtacgc agtggcgaga gttccccgtt gcgtcgggcg atggtgcggg cgaggttctc
     1741 cagccgcccg acctctctta tcctctcgat ccgaccctcc gactggaggg ccagccccgc
     1801 cgaatcgtac ccgcgatact cgaggtaccc gaggctctcc atcagcacct ctacacagcg
     1861 gtccctaccg gcgtaaccca cgatgccgca cattgtccta cctccctgtt tatccacaga
     1921 tggaacccgg gagggtagag agggttgaca gggcgacaga aagccctgcc cgggcccgaa
     1981 cggtgctttg tccccggcgt caccaccggg atttgtcacc gccctgacga ccgcccgaag
     2041 ccgagacgca cccgccgaat ggttcgatga acgtcttcct cgtcgactcc ctcgttagag
     2101 agtcctggcg ctcttgttgt tccctacccc gcaagcagct tggatcccat gaagcttgct
     2161 ggccgtctta cgcaaaggtt cgtttttctc tggctcgcct ccctctcctc tccggaacga
     2221 cccgcttact ctatcgtctg cgggacgaaa aagaaaccct gatcctctac agtatgttca
     2281 ggcttatgat gcccgtcagg gcgagcaggg cgatcacgat tggctggtgg atcaggtata
     2341 taaacagcga gtttcttccc agcggcagga gcggcctgcc tagaagcggg gctctatccg
     2401 gcaggcccgc cggcctgtgg ccgtcggcgt agaccacgtt cccgaagtac agcccgatca
     2461 ggactacgcc aaaccagggc aagagcggcc tgtagtccgg cataatcagg ccctccggta
     2521 ccacgccgaa gggcaacagc cagtacgttt ctgagatgta atcctgcgcc agcatgtact
     2581 ggccagcgac gaagacgatg aggcccagca cgaggttggt gacccgaagc ttcaggaagg
     2641 ggtaggcgag gatgacggag accccgatca ggtgcagtat cccgaacgcg acgaccccca
     2701 tctggagcgc caggaagatc ccggtcagga tcatgccgta ggcgaggatc ctcaggcccc
     2761 ttgccagata cttcccgaag agcctccacc ccgaaaggct cgttctcgac cggctgatgg
     2821 cgagcgagac gccgacgagg aacagaaaga ggccggccgt aacgtcggcg aagaccgccc
     2881 agcttcccga ggtggcgtcg atgtcgtacc ccccgaggac gtccaggtcg taggtggtgt
     2941 ggtagacgac catcatgatt atggcgacgc cccgcgcggc atctacctcc cagaacctgg
     3001 ccaccttctg cgccgcgcct ctcttcgtgc cccgctcctc tgcagttcgc gccatcaaca
     3061 ttgggctttc agcaagatca acgggctgaa agccaacggc tgatggttgg caaagaggtt
     3121 cgatcccttt gctcaccgct acatgatcgg gatcataccc caccctcgcc accgccgatg
     3181 ccggcacatg ggttaggatt gggtgatgga agcggacttc accatcaggc cgggacggaa
     3241 ggaagacgcg ccagaggcgg cccgcctctg gatgcagagc gccgacgagc ataccgccca
     3301 cgatcgagtc tacgagacct cgccagacgc agagagaacg atgcgccgct ttctggccga
     3361 cgtggcgaac agcggctact cgtttctctt cgtggccgtc accgaagatc gtacggtcgg
     3421 cttcatctcg ggcgagctgc gccagggctc ccccaccttc ctaccgaaga cctgggcctc
     3481 cgtggacgac gtgttcgtcg aacccgagca ccgcaaccgt ggcatgggcc gcgccctcct
     3541 ccgtagcgtc caggcgtggg ccagggagcg agacgcggac ggcatctctc tccaggtcgc
     3601 agccgccaac gcgcgcgggc gcaagttcta cggggaactg gggtttaggg aagtctcggt
     3661 ctacgagctt ctcgagtttt agagctgtta gctttcagcc ttttatgttg ggaacatcga
     3721 tgggtaggga tcgggagagg acccttacgg tttcctctgg ctcgtagcct acggcggcgt
     3781 agaatgattc ggccgttttg tcctctacct gtacttcaag gtaagaaatc cggtgtgctt
     3841 tgcaacgttt gtctgcagct ttgaggaggg cgcggcccac gccctggcgt ctggcttcgg
     3901 ggcggacgta gaggtcttcg atggaggcga agagggctcc ggccgcgatg ctgggacggt
     3961 aggccaggac ggctacgccg agggtgcggc cctcggatct ggcggcgagc acgtccatgg
     4021 tcccggttcg gacgtgctcg cgcagacttt cgatgaaggc ttcggggacg ggctcgccgt
     4081 ctctaaggga tgtttcgagg agcgacaggg gttcggcgag gtcgggagcg ggggtgacct
     4141 ctatggggct caacgtcccg aggtcgttgc gaggattcgg gccgctgcct cgatggcggt
     4201 ttctatgtcc tcgtctccca cgtcgaggtt ggtacacagg cgcacgaagc ctggttttcc
     4261 aagggtggcg agcacccctt ccgttgcgag ggcccgcagg aactcggggg gatcatcgac
     4321 ggcgacgagg accaggttgg tgtctggttc cctggcctcg tagccagcct ctcgcaggcc
     4381 cgctgcgagg ttgcgggccc gttcgtggtc ttcggagagc ctccccgtgt tgttctcgaa
     4441 ggcgtagatg gaggcggcgg ctatgatgcc ggcctgacgc atgccgccgc cgaaggcttt
     4501 acgggcccgg cgggcttctc tgatggtctc tccgtcgccg gccagcaacg agccaaccgg
     4561 cgccccgagc cccttggagg agcacactga gacggtgtcg gcgtgggcgc accattccct
     4621 ggccggtatg ccggtggcgg cctgcgcgtt gaacagcctg gccccgtcga ggtggacctt
     4681 gaggccgagc tctctcgcgg tctcggccac tgcggcgaag tcttgcaggg ggaatacctt
     4741 gccgcccgcc gtgttgtggg tgttttcgag gcagagcagc ctggaccgcg ggaagtggac
     4801 gtcctcggac ctcacggcgg cccggacggt atcggggtcc aggatgccgt tgtccccagg
     4861 gagggtgcgg agctggacgc ccgagagcat cgccggtgcc ccggcctcgt agacgaagat
     4921 gtgggagccc tcgtgcagga gcacctcttc acccctgttc gtgtgtacct ggaccccgat
     4981 ctggttggtc atggtcccag atggcgcgta cagggccgcc tccttgccga gcaggtcggc
     5041 cacatactcc tccaacctgt tgacggtagg gtcctccccg aagacatcgt cacctaccgg
     5101 tgcctccagc atcgcccgcc gcatggcttc cgtcggccgg gtcacggtat cgctgcgaag
     5161 atctatcacg gtctacctcc atcatgtcgt gcgagcaccc gccgggtggt agcgaaatgg
     5221 accttggcca ccccggcaag gccttcctcg cccagttcct cgaccacccg gcgacccgca
     5281 tccacgacgt gcgtggcgcc gaggggcaat cgaaagccta cctgctccga aaggcgttct
     5341 atctcctcga gttgccgcgc ccgcgccacg atggaagccg ccgcaacggc cgcgtcgtcc
     5401 tcggccctgg gacggacctc cagccgcacg ccacggggga cgaacggtcg cagatgatcc
     5461 cgggccgacc tggcgaactc gtccaccacg acgacctcga cctctcctgc aagctcgacc
     5521 acgatcccgg cgtcgagctc gccgagcagc ccattgatgt tgcccgcggc ccccctgcgg
     5581 acctcatact cgcgtggcga gagcgagacg acgcggacgt tctcggggcc cacggcttcc
     5641 aagatgcgct ccgatatcct tcccacgccc agaacaccga tctcctttga atccctgacg
     5701 ccgatctctt gcaggctctt cgcctgcgct tcgccggtga tcctgacccc ggcgacgacg
     5761 agggggccga aatagtcccc cttcccggct tcatcgatgc ctagccttgg cgtgcggtcc
     5821 aaaaccggca tactccccga agaccgggag gatgttcgcg tggggttccg cgtggccggt
     5881 gcccctcgct gtgaggtgcg ccagccgtcg agcaggtctc gcaaccctga agctttgcct
     5941 ccggcagata ccttgcccgt gtgatagacg ttcagtttgg cggtctcgcc tgcacgggcc
     6001 aggtcgtact gggtaccatg gtcgatgggc ctgctgctcg tgacgctcac gccctccctg
     6061 gcgaggagca agcgcaggcc gtcgggaagg tctgtacccg acccgttcaa cggacctcga
     6121 agccgctcag gtcgccggcc gcgtcctcga tgtccgtatc gacgttctcg acggaggcct
     6181 gccgggggcc ttcctcgcac cagcggagca tctcctttac ccggtcaggg tgaccctcga
     6241 agacggcctc caccctgccg tccggcaggt tcctgaccca tccggccagg gtcagatctt
     6301 cggccttctg cctcgtagcg tcgcggaaga agacgccctg cacccggccc gatatgcgta
     6361 cgtaggcccg ttctagatct ttgccttgca tctctcttac ctccgttgga aacctggccg
     6421 gggcgggttt acccgatatg ctagcctaga gcggccatga aagcacagag ggaaaccgta
     6481 ggctacggca cggaaggcgg cgagggctcg tccagggttc tcctgaccct cgtcatcccg
     6541 acgcgcaacg aggtggacaa cgttcccgag ctcatggccg ggctacgtga gagcctcatg
     6601 gccgtcgatt accgggtggt cttcgtggac gactctacgg atgggacgcc caccgtgata
     6661 aaggacctcg cccgggaaga cgcgcgggtt agcctggtcc gcagggaggg ttcggagagg
     6721 cggggcggcc tctcgacggc cgttacgagg ggcctggatc tctcctccga agtcagcgag
     6781 tacacgtgcg tgatggacgc cgacctgcag catccccctg agaaggtaag ggagatgcta
     6841 gagaccgccc ggcacacaga cgcagacgtt gttgtggcta gccggtacgc ggaggggggc
     6901 agctacaccg ggctctctgg aacgatgcgg cgggccgtgt cggtggggag caagtacctc
     6961 gcccggatcg tcttcaagga ggcgcgcaag accagcgatc cgatgacggg gttctttctc
     7021 ctcaagaacg aggcgatctc gggcatccag ttcaggccaa cggggttcaa ggtcctgctg
     7081 gagatcctgg tgtgcgcccc cgagttgaag gtggtcgagg agcccttcga cttcagggcc
     7141 aggcatgccg gggtctccaa ggcgaccctg cgccagggcc tcgactacct gatccacacg
     7201 ctcagccttt tctggtacgt gccggccgcg ggccgcttct ggaagttctg cctcgtcggg
     7261 gcctcgggtg tgctcgtcaa catggttacc ctcatcgtgc tcgccgaggt cttcgacgcg
     7321 cacaaggtta ttgcctggat gttcgccgtc gggctctcca tattgagcaa tttcctgctc
     7381 aacaacgcgt tcacctggcg cgacgtaagg cactcgagca ggatccactt cttcctgcgc
     7441 ggcgtgctcg cctaccccgt ggcggtcatg ggcatcggtg ccaacttcgc agtctggtat
     7501 ccgctgatca agtacgtctc ggatgcgttt ccctactacg ctatcttcaa cctgctcggc
     7561 ataatcgctg gtacgagcgt gaacttcatc ctctcttcac ggctcgtgtt ccgcccctcc
     7621 gaacagaaga gcctgaaccc agacgccccg agagggcaga tcacggaagg gataagacgc
     7681 gagctgaggg cagacagggt cgccctgata ccggtctccg gccttacgcc cctcggtacc
     7741 tacgaccatc aggaactgac cgttaccgac aggggtgtga tagacctcgt gatcaagacc
     7801 tcccacccca ccctgaccgt gaccgggccg aggcggctcc cccaggcccg caccaatgcc
     7861 cgccactcta actccctggc ggttcccatc ttcgacgaag aacgcaccgt cggcgtcgtc
     7921 tacgctaccc gtaactccac agaacctttc acggaggaag accttcactg gcttaccgcc
     7981 tacaccagca cggcatcgga gttgttcact tgagccgatc tgctctgtga cgcggtcagc
     8041 cgaaaaatag gggcatcccg gcagtgccgc caggatgccc cggtcggccc ctccccataa
     8101 ggagccgctg aaaggacctc atcacccctc cccgacgatg aagtccgttg cgatcccagt
     8161 gccttgaccc aagctttaaa ccgagccccg ggtgacacga gaccgcccga tcaaaaacct
     8221 agcatcccat ccaacacctc gcaacccacc acaatcctta tacctatgcg gcaaaagacg
     8281 ttcattgctc ctccagatac gacatgatgt agtacgtaat aaagctgcac aatatgttga
     8341 gtctctacca aaaactcaat ctgcacggaa agctacatcg accgtcagtt caaggtcagg
     8401 tgagtttaag gttgagatgt actccttggg gtttggggag tctggcgaga tgggcctggt
     8461 cgagaagctg ggcgcagaag gcgttggcgc gttttatact accggcatga ggccggtttg
     8521 ctttctatcc gacttcggtc tcgccgacga cttcgtgggc acgtgcaagg gcgtgataca
     8581 caggattgcg cccggcgtat ccgtggtaga tctgacacac gcggtgccag atttcggggt
     8641 cgagacgggg gctgagttgc tggagcacgc caccagatac atgccggcag acgcggtgta
     8701 cctggcggtg gtggatcctg gcgtcggtac ggagcggcgg gggatcgcgc tgtctaccgg
     8761 gggtggggcg atgatggttg ggcccgacaa cgggttgctc atcgccgcgg cggagtccct
     8821 gggcggcatc tccgaggtgg tcacccttac gaaggagcgc ttccacctgc gtcccatctc
     8881 caacacgttt cacggccgcg acatcttcgc cccggtggcg gcccatctcg tggccggggt
     8941 gggccttcag gagtttggag agtcggtgga ccccgcttcg ctagtccgtc tagagacggg
     9001 aagcttcccg tccgagcggg gcggcgggat gactacccgc atcctctccc tggaccattt
     9061 cggcaacgcg aggctttcgg ccaccacgga gcaggcgggg ctggagtacg gcgacgccct
     9121 gaaggtagat gccggagacg gcggcatgtc cgtccggtat gtagagacct tcgggtcagc
     9181 gagggcggga gaactggtgc tcgtgcctga ttcccactgg cgcttgagcc tcgccataaa
     9241 caagggcaac gcgggccagg cgctcggttt gagggtcggg ggaacggtgc ggctcatccc
     9301 tgcagagagc gatgatggct gatccccacg gtcagcggtc ggggccgccc gcagagaagg
     9361 actccacgtc tccctcgccg cagcagcgaa acacgatgct gcggtccgcg gccctacggc
     9421 cgatcaacgt catggtcctt gtgatcgggg tggtcatgtc cgtaatcacg gcggtctggt
     9481 ggttgctgcc cctgacgttc gtcacgtacg cttccctggt cttcctgtca gcgagagacc
     9541 ccatcttcca gcgcaaggtg ctcggcggag gcagaagcgc ggcgctaccc cccaccacac
     9601 gggacatcga ggtctcgcca gagcgtcggg cgcgttggct tccccgcggg gagacccgcc
     9661 ggaaggtgga ggagacccta caggtgtacc gcaaggtggt cgccgccatc gaggcctccg
     9721 acgacgtgac cagggccgtc ctcgacgacg ccgtacccag gttgcacgcg gccgcggatc
     9781 ggttggtcga tgtagcagag gcaagagaga aggcaaacga agtggccggc gagctacgtc
     9841 aggaccccgg agcgacagcg gactctacca ggcaggagaa cctgcaccac ctggaagagc
     9901 aaataatagc tgcagacgcc gagatctccg gcacctcaga ggagcttgtc aacctcaggg
     9961 ccaaggtcgt caggctctcc atagacgcga acaaccctac ccgcgcaaga gacctcaaca
    10021 cttccctgga cagcctcaac acccgactcg aggccctgag cgagacaatg tctccacgcg
    10081 aagaggggta gagcacgctc cgcctacgct tgtcagcaca ctacggccct acgggcctcc
    10141 gtttgtcagc acgctcgggc taacgccctc gcttgtcagc tcgcttcgcc cggcttcgcc
    10201 gggctcgctt tcagccagat cccgttgcgg ccagccagac gccgctttga agattcgatg
    10261 accgggaaac gtgggttgtc ggagccaccg atgcgaatcg ggacgaccag ccgaaatcga
    10321 gagccttaag gctcgaagcg ttgctgacga gcgtaggcga agccggagcg tgctacgcgg
    10381 agctggcgtg ccagccgcgg tcttcgggct tctcctcgac gatctcgggc tcgcgcgtgg
    10441 acctgcgcac cggtacaccg cccagctcct ggcgggactc ttctacggag atgagcgagt
    10501 cctggaacca gttctccagt tgctccatca cccgatccct gtacacttcg gagccgccag
    10561 acacctcttc ggcgtagcga tcagccccgt ctataatctc ccccgcccta cgctccgcgc
    10621 ggcttacgat ctccgtctcg gggaccatgg cgttggcgcg gtggtgggct tcctcgacta
    10681 tcctctgcgc ctcctcctcg gcagcagcga tcgtggcccc acgctcccgc cggagcgcct
    10741 cagcctccga cagctcgggg ggcagagaac cccttagctc gtccagcatc acaagcaggt
    10801 cagaccggtc taccacggcc ctccccttgc tcatcggtac gccggatgca tcctgaacta
    10861 tggcctccag ctcgtccaat accccgctta tgtactgcat gtcttctctc atgagcccaa
    10921 ccctagcacg ctctctcagg ctacgctagc ctttctgaaa gtatttttcg atgctttacc
    10981 ttaacttcag gtctaatata atctcaaggt caagtgtaaa gctgagaggg gttggcaacg
    11041 ttattttgca tacaccaggc ttcccggttt ttcgctttat tagtgcggtt tcaggactta
    11101 tccacaggca agtttcagag aagtgacacg ctcaaccggc acgagaggca gggagagaga
    11161 tcggggctcc ccctgccgca acgttgcctg gtcccatacc ctacacccgg gattacgagt
    11221 agccctctta cgggggtgcc acgagccacc ggcagggagt caggaacgca gggccagggc
    11281 tctctatatc cgtcggttcg ccacgccggc ggcgatggcg gcgacgaggg gggcgatcag
    11341 ttggtaaggg ttgatgctac cggcgagcag gttcggagcc atcagcacca ggaagggaaa
    11401 gagcgtggcg aacacggaga tggcgatggc gatcggtcca ccattacgac ctccggcgcg
    11461 gttgatcagg gtccccgccc cgtgaccggc agccacggag agcaacaggg tgaacgggag
    11521 ttggaagagc acccagacca ccggtacgcc ggcgacggcg accgcgaggc cggcgaggac
    11581 gctcctgagg agctggttcg tcttcatcac gccggcccgt gcgccgcgag gcagtctggc
    11641 gtcgtcgggg cacttgatcc ccacatcggt tgccaccatg cactccgtgc agatgggacg
    11701 tccacacgtg gcacaggaga ctcgtgtctc ccgcttcggg tgcctgtaac agtgcatcgg
    11761 ctccgtcact tgcgaccctt tctcgcgctg cgcgctaact cgcttcgtgg catcaagacc
    11821 agctccctct tgtagctctg gtggacttcg gcgacgagcc tgtgatgttc cagctccttg
    11881 cgctccagct cttcacgcaa ggcacggttc tccttctcca gagtggccac ctggcgctcc
    11941 aggtcgagaa ccttgctcac gccggccagg ttcatgccca gttcctgcgt cagcatctga
    12001 atgtagcgtc ccagttcaac gtcttcctgg gagtagagcc tggtgttctt tatgctcttg
    12061 cgcgggcgta gcaggccctt ctgctcgtac atcctcaacg tctgcgggtg aaccccgatc
    12121 aactcggacg ctaccccgat catgtacacc ggacgccttc tgatgctcac gacgacccca
    12181 cctccttgaa gaggtcctcc cttacatcct cgtcccgctc ttcggcgaag gcttccagga
    12241 tctcccgttc ccgccgtttg accttcttgg gtaccaccac gttcacccgc acgatgagat
    12301 cccctttctc cccaccgttc gtcttggccc tcggcgcccc cgcacccctc accttgaatt
    12361 gcttaccgtc ctgcgtaccg gcggtcagct tgagcctcac ggtcccccca cccggcctcg
    12421 ggacctgtat ctcagccccg agcgccgcct cgacgaagct gacgggaacc ccgacgacaa
    12481 agtcgtccgc tttgcgcttg aacaccggat gctcctccac cagcgtcctg acgtagaggt
    12541 cccccggcgg cccaccgtcc ctgccggcgt ggcccctgcc agggatcctg accttcatct
    12601 tgtccctcgc cccagccggt atccttacct tgacctgctt gctcctgcgt actcggccgc
    12661 tccccccaca cccggcgcac ggcttctcga tgatcgtgcc atctccccca cacccggaac
    12721 agggcgtcga gagcgcaaaa aacccctggt cacggctctg cacccccctg ccgccacact
    12781 ctggacacgt ccggggcgag gtgccgggtg cggcacccgt gccaccgcac tcgccacaag
    12841 cctcttccac cggcacaccc acccgtgtag tcacgccgct caacgcatcc ttgaaactca
    12901 acctgacgtt tatggttatg ttctcgccct tggcctgggg tcgctgggta gtggtagtac
    12961 gcccgaatat atccccaagg ttaccgaaac cacctagcag gtcggagaac tctccgaagt
    13021 tcgcgttgcc cggccgcgcc gtttcccccg agccgaagaa cgtacggggg ccctcgtcgt
    13081 attcccttct cttttcgggt ttggagagga tttcgtaggc gtgttgaatc tctttgaagc
    13141 ggtcctcggc cttcttgtcg ccggggttgg cgtcggggtg gtacttgcgc gccagcctcc
    13201 ggtaggagcg tcggatctca tcctgggaag cctccctgga gacctctaga accttgtaga
    13261 gatccaactt ctccataccc taaacctgct ttgcaaccac gactttcgcg ggcctgatcg
    13321 gcttcccgtt caagaggtag ccccgctgga aggtctggat gatggtgccg tcttcgtgtt
    13381 cttctgaggg ctgtcccata accgcatcat ggatgttcgg gtcgaaaggc tcgccatccg
    13441 agaccacggg taggagccct tcctcagcaa gaacctctgc gagctgctcg cgggtggcct
    13501 gcacgccctc gcgaatatct ccccccgcct cgagggcgcg gtccagattg tccaggacgg
    13561 ggagtagctc ctgcaccaga cgctccgagg ccatggacag gattcgggag cgctcccgct
    13621 cctggcgctt gcgcgagttc tcgaactccg ccttgagacg acgcagggaa tccaggtact
    13681 ggtcacgctc caggcgtgtg gcctccagct cctccgtcag cctggcaacc tcatc
//
LOCUS       DBNGVD010000510         4647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1587133, whole genome shotgun sequence.
ACCESSION   DBNGVD010000510 DBNGVD010000000
VERSION     DBNGVD010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1587133"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC16CM_13820"
     CDS             complement(<1..270)
                     /locus_tag="AC16CM_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140958.1"
                     /translation="MIERANRETSLINVYLIVAMFVATLLIFTACAPAGGDKGGGDQV
                     IAGSPKPRDDGPVPLQEQADPDGAIGDRVKAGDLSFRIFDARAKDR"
     gene            510..1091
                     /locus_tag="AC16CM_13825"
     CDS             510..1091
                     /locus_tag="AC16CM_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HIR3140959.1"
                     /translation="MESGLPDEAEKPVNAGIIGEIERRAIVIADYDPAWPRRFALEEA
                     RIRGALGPAALSVEHVGSTAVPGLAAKPIIDILLVVEDSSDEGTYLTHLEREGYVLRV
                     REPYFEEHRMVRTPEKDVHIHIYSPDSREGERLILMRDHLRHDRADRELYASTKRELA
                     RRDWPTMQHYADAKTQVIQEILVRARRSARKPR"
     gene            1214..1912
                     /locus_tag="AC16CM_13830"
     CDS             1214..1912
                     /locus_tag="AC16CM_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140960.1"
                     /translation="MATLLFTAFALGLASLDFTATVLAVGALGAGARNRALLAFGCVC
                     ILGTLVFGATLSLVIGPRIAGLNLASLLPHDHAEDLIAAIVEVLLGAGLVTWGTVRVL
                     RPHTIPPKPTVPRGVGPASLAAVGVLFALAAILDPPFLSLVVIAGRSEHFWPVVAAHS
                     TWVCVSHAPLILLLALALSTDHELVVNRFQTLWTRIRPIMVRLVTIVVLLAGALLLLD
                     ATWFFLTGDLFLPT"
     gene            complement(2622..3557)
                     /locus_tag="AC16CM_13835"
     CDS             complement(2622..3557)
                     /locus_tag="AC16CM_13835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3140961.1"
                     /translation="MERLALKARAICNPASSGGGYEPTDLQRELEGYDLEWITTQSPG
                     DATEAAREWREGLLIVVGGDGTISEVVNGLGMAGFPAGVTLAILPAGTGNDLAATLAI
                     PEEPSESRAVLHQNRVRTLDAVRVRSDSRGEQFFINVATGGMGAEISGAADGEMKRKW
                     GKLAYLRASLEVAREYEAKEVTLTLDGIERQLRAVSMAIGNCRYAGSGWLAAPRANPE
                     DGLLDLVVIEDIGVLEVFKLAPAVLANSDYLDKEGVFVARAKEIRVEASPGSLEFTVD
                     GEVIGNEPAVFQVIPKALKVVVGPGYVPEPRVSHR"
     gene            complement(3842..4000)
                     /locus_tag="AC16CM_13840"
     CDS             complement(3842..4000)
                     /locus_tag="AC16CM_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140962.1"
                     /translation="MAEMADIGKKIKDAGTKAAKDKISGSGKDDKRTEGSNSGADKAM
                     KTAKKFLK"
     gene            complement(4031..>4647)
                     /locus_tag="AC16CM_13845"
     CDS             complement(4031..>4647)
                     /locus_tag="AC16CM_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140963.1"
                     /translation="TGIDRRDNGGEADWMALSATQESVQTAMGYALEAQEMNTRILRL
                     TSQAWAESFRLQSTLFQGMAQSFFGQWENSLASATSVESLGQEQSRSELTGPTTDSAE
                     DTEVAVDEAHQKTEKEKGEKQGADEVATEMRRAEAVAEELQKSTATATFPKEGYDEMD
                     VGEATEYLNGLSVDELKRLRKYEKYNKNRRSLRKEIKQKIKIAS"
BASE COUNT          807 a         1543 c         1362 g          935 t
ORIGIN      
        1 gcggtccttc gcgcgcgcat cgaagattct gaacgacaga tcaccggcct tgacgcggtc
       61 gccgatcgcc ccgtccgggt cagcctgctc ctgtaagggg accggcccat catcgcgcgg
      121 tttgggggaa ccggcgataa cttggtcgcc ccctcccttg tcccccccgg ccggagcgca
      181 ggccgtgaag atcagcaagg tcgccacaaa catggcgacg atcaggtaaa cgtttatgag
      241 gctcgtctct ctgttagctc gctcaatcaa gacgagagga ttatacgacg ggtgaactcg
      301 aggttggtct taccagcgat cgcccggtgc gtgaaggaca cgtgaggcac gggcggcccc
      361 acaccaccgg tggcccggga ggcgccgcgc gtagccgcac cgctttcggg ttcagacgca
      421 ggcgcccggt ggcggtcgac atcccacacg agcctgttgc gaccggcggc cgcggagggt
      481 acctttgcgg ggacgtcgat aacggagaga tggagagcgg tttgcctgat gaggcagaaa
      541 agccggtgaa cgctggcatc atcggcgaaa tcgagcggag ggccatcgtt atcgcggact
      601 acgatccggc atggccccgt cgtttcgctc tcgaagaggc caggatccgc ggggcgctcg
      661 gcccggcggc tttatccgtc gagcacgtcg gctccaccgc ggtacccggg ctggccgcca
      721 agccgataat cgacatactg ctcgtcgtcg aggattcgag cgacgagggt acttacctaa
      781 cccacctgga aagggaaggc tacgtcttgc gcgtgcgaga gccctatttc gaggagcaca
      841 ggatggtcag aacacccgag aaggacgtgc atatccacat ttactcgccg gactccaggg
      901 aaggcgaacg tctgatcctg atgcgcgatc atctgcgcca cgacagagcg gaccgtgagc
      961 tgtacgctag caccaagcgc gagctggcgc gaagggactg gccgaccatg cagcattacg
     1021 cggacgccaa gacgcaagta atccaggaaa tcctcgttag agcccgcaga tcggctcgaa
     1081 aaccccggtg atctgactcg tatggccttc ggtgaccggc cggtcatcgg gtctccgggc
     1141 aaaattacgg cccaatcggc cgaatgcgtg ggcccgtgcg cgtcggcatc ccgagaaacg
     1201 ctatcattcc cgaatggcca cgctgctgtt caccgcgttc gcactcgggc tggcgagcct
     1261 cgacttcacg gcgaccgtgc tggcggtcgg cgccctcggc gcgggggcac gaaatcgggc
     1321 gctcctcgcc ttcggatgcg tgtgcattct cggcacgctg gttttcggcg cgacgctctc
     1381 gctggtgatc ggaccacgga tcgccggcct gaacctggcc tcgcttctgc cccacgacca
     1441 cgcggaggac ctcatagcgg ccatagtaga ggtcctgttg ggtgccggcc tggtgacctg
     1501 gggaaccgta cgcgttctca ggccccacac catcccacca aaaccgaccg taccgcgagg
     1561 ggttggccca gcttccctgg ctgccgtcgg cgtcctcttc gcgctggcgg cgattctcga
     1621 cccgcccttc ctgtcgctcg tggtgatcgc cgggcgttca gaacacttct ggccggtcgt
     1681 agccgcccat tcgacctggg tgtgcgtcag ccacgcgccc ctgatcctgc ttctggccct
     1741 ggcgcttagc accgaccacg aactggtcgt gaaccgattt cagaccttgt ggacgaggat
     1801 acgcccgatc atggttcgcc tggttacgat cgtcgtgctc ctggcagggg cgctgctcct
     1861 cctggacgcg acgtggttct ttcttacggg cgatctgttt ctgccaacgt aaccacaggc
     1921 gtcggccggc gcggcacttg ctcggttcga cggcggaccg gcgttgcgcc gttccagcgc
     1981 gacagcatct tcgctacgca attcgaggat gcgccgagtc tgggctgatg ccatgactac
     2041 cgtgcgagcc gggccgctgg cccgctggag cgatgaacca gaggaggagc gtatgtcggt
     2101 cgtcggagaa gcaagccacc gtctacgggc ctccggacga gggtgaaaac ggtctggttg
     2161 ttgggcgata cctaccattc tgaaggtgtc cagggagcag accggcgggg ccctcacgct
     2221 gctcgaggcg ttggcacctc cgggcagggg gcgaggcaca aacaccatct cgacgacgaa
     2281 gtgttcatcg ttctcgaagg cgaactgctg gtcacagtgg acggtcgcgc tcccgttgcc
     2341 ggtcgatacg gttatatccg tgccgaaagg cacccggcac tcttacgccg tctcagggac
     2401 gacgcccgcg ggaatggacg agatgttcgg cgagataggt aagcctgccc agcccggagt
     2461 gacggcccgc cggccagcga ggaagacgtc gccagactgc tctcgattgc ggcaaagtac
     2521 aacttcacga tcgtgccacc aggagaggca taacccctct cgttcgcgca acgtcgcaaa
     2581 ggggaagtat tgggatcccg ttttgcacgc gctcctccag gttacctgtg gctcacccga
     2641 ggctctggta cgtacccagg gccgactacg accttcaggg ctttcgggat cacctggaac
     2701 accgcgggtt cattccctat gacctccccg tcgacggtga actccagact ccccgggctc
     2761 gcctccaccc tgatctcctt cgcccgggcg acgaagaccc cttctttgtc caggtagtcc
     2821 gagttggcca gaactgcggg ggcgagcttg aacacctcca gtactccgat gtcctctatg
     2881 accacgaggt cgaggagccc atcctcagga ttggccctgg gggccgcaag ccagccgctt
     2941 ccggcgtaac ggcagttacc gatggccatg ctgaccgcgc ggagttgccg ctcgatgcca
     3001 tccagcgtca acgtaacctc cttcgcctcg tactcgcggg ccacctcgag cgaggccctc
     3061 agataggcta gtttccccca cttcctcttc atctccccgt cggcggcacc cgagatctcg
     3121 gcccccatac ccccggtggc cacgttgatg aagaactgct cccctctgct atccgaacgg
     3181 accctgaccg cgtccagggt tcgtacccga ttctggtgta acacggcccg ggactccgaa
     3241 ggctcctcgg gtatggcgag agtggctgca agatcgttgc cggtccccgc cggcaagata
     3301 gccagggtaa caccagcggg gaaaccggcc atgccgaggc cgttgacgac ctcgctgatc
     3361 gtaccgtcgc ccccgacgac gatgagcaag ccctcacgcc actccctggc agcctccgta
     3421 gcatctccgg gactctgcgt ggtgatccac tctagatcgt agccttccag ctcccgttgc
     3481 agatccgtgg gctcgtagcc acccccactt gacgccgggt tgcagatcgc ccgcgccttc
     3541 aatgccaagc gttccataac ctcacctttc gtcagtggtg gttgttcccc gggtcacagc
     3601 gattgacgcc gttcccgtac gtggcaagag ttgcgctccc gtcttgcagg agcaccgcct
     3661 tagaaagctt cactcattct agagaagctg caatacgagc aatatcacca cgattacaac
     3721 aacgacgatt atcaacgtgc gtatcatccc ataccccctc tcgcagacat gatccccaac
     3781 gaagcgcaaa gccgacaggt cggtagcccg cgccgagccc cgacctgtca gcacgccgct
     3841 attacttcag aaatttcttg gcggtcttca ttgccttgtc cgcaccgcta ttcgagcctt
     3901 ctgtgcgctt gtcgtcctta ccacttccgg agatcttgtc cttggcggct ttcgttcctg
     3961 catctttgat cttcttacct atgtcggcca tctcggccac ctttctctgt aggtgtacgt
     4021 ttctgttccg ctacgaagcg atctttatct tctgcttgat ctctttacgg agagatcggc
     4081 gattcttgtt gtacttctcg tacttcctga gccgtttcaa ctcatcgacg ctgaggccgt
     4141 tgaggtactc ggtggcctcg cccacatcca tctcgtcgta gccctctttc gggaaggtag
     4201 ccgtcgcagt actcttctgc aattcctcgg cgaccgcctc ggccctccgc atctctgtgg
     4261 ctacctcatc tgccccctgc ttctcgcctt tctccttctc ggttttctgg tgggcttcgt
     4321 caacggctac ctcggtgtcc tccgcgctgt cggtggtggg ccccgtgagt tcggacctgc
     4381 tctgctcctg gcccaggctc tctacagacg tcgcgctcgc caacgagttc tcccactgcc
     4441 cgaaaaacga ctgggccata ccctggaaga gcgttgactg gaggcggaag ctctcggccc
     4501 aagcctgcga cgtcagacgc aggatcctgg tgttcatctc ttgagcctcg agtgcgtaac
     4561 ccatcgcggt ctgtacagac tcctgggtgg ccgacaacgc catccagtcg gcttctcccc
     4621 cgttatccct cctgtcgata ccagtga
//
LOCUS       DBNGVD010000511         2606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2682025, whole genome shotgun sequence.
ACCESSION   DBNGVD010000511 DBNGVD010000000
VERSION     DBNGVD010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2682025"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CM_13850"
     CDS             complement(<1..815)
                     /locus_tag="AC16CM_13850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3140964.1"
                     /translation="MAQEVRYGKVENLKGIVWHGPEQMSVDEVPEPSVEPGTVVVRPK
                     ATGICGSEIEGYLGRMGNRTPPLVMGHELAGTVMEVGEGVDEGLVGHLVAVNPLSSDG
                     TCPLCRAGLTNLCPNRKLVGIHSPGGFAEFTLAPASNVYPLPEGVEARTGALAEPLAN
                     GVHAARLGLAGRSVEHAVVIGAGTIGLMCLQAAVLDGIPEVHAVEPVEGRRGQALALG
                     ADAAHASGEEAREALDGPTGGLGVDLVIDAVGAEATRRMALELLRPGGRAVYIG"
     gene            904..1725
                     /locus_tag="AC16CM_13855"
     CDS             904..1725
                     /locus_tag="AC16CM_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3140965.1"
                     /translation="MDAEGPRERLLIGEVAGLLGVTPKAIRHYEKLGLIENPERSESG
                     YRLYTADGLLRLQQIKRLRSLGFSLERIKRVLGESGPGVELGSVLEALLVEVESQIEL
                     LEQRRASLRRMLSEGDPSEADEEPYILELARQHFGERLGELDPEVLDQEKRFWATLDA
                     FRWPREFRSFQETLVLYLADHPEQYEQLLILEQRFATLAHRPENAREVEQLAEEYAAY
                     FEANPLPGELSKGADWGSGPMEAALSGVVLSAMSPAQKRCMQLLQEHVLGNEGER"
     gene            1722..>2606
                     /locus_tag="AC16CM_13860"
     CDS             1722..>2606
                     /locus_tag="AC16CM_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140966.1"
                     /translation="MKRALKVLVPLVVLAEVILVWSGVMDLGDAVILVAGLEALLFVA
                     GLGGLVLVVGRYRRGRRAGMDAWRALEDGLSLVLPRAVARLVVKEPRLLVCLFRWTIW
                     RVRLTDEEFAYHKRSLLGAIVPLLVVSAPVELILVHMLALAFSPWWWLKWALLVLGAY
                     ATLWLLGLYASLVTLPHRLEEEGLRIRYGLLAEGFFPYQEIEATSRRDLEAPTPGDGL
                     SHDSGEETLYLASGGKTDVSLRLRVPRSMSGFSKDTGPALCVCLAVDEPDRFVRELRL
                     RFGAPTRNPPSVLGERGTE"
BASE COUNT          421 a          861 c          860 g          464 t
ORIGIN      
        1 cctatataga cggcgcgtcc gcccggccgc aagagctcga gggccatccg gcgcgtcgcc
       61 tcggccccca ccgcgtctat gaccaggtcg accccgagac cccctgtagg cccgtcgagc
      121 gcctcgcggg cttcctcgcc ggaggcgtgg gcagcgtcgg ctccgagggc cagggcttgc
      181 ccgcggcggc cttccacagg ttccacggcg tgcacttcgg ggatgccgtc gagaaccgcg
      241 gcctgcaggc acatgaggcc gatggtgcct gccccgatca cgacggcatg ctccacggat
      301 cggccggcga gcccgagccg cgcggcgtgg acaccgttgg ctagcggctc ggcgagcgcg
      361 cccgtgcggg cctcgacgcc ttccgggagc gggtagacgt ttgaggccgg tgcgagcgta
      421 aactcggcga agccgccggg ggagtggatg ccaacgagct tccggttcgg gcagaggttg
      481 gtcaggccgg cccggcatag ggggcacgtc ccatcggagg acagcgggtt gaccgccacg
      541 aggtgtccga cgaggccctc gtcgacgccc tcccccacct ccatgacagt tccggcgagc
      601 tcgtggccca tcaccagggg aggggtacgg ttgcccatcc ggcccaggta gccctcgatc
      661 tccgagccgc agataccggt cgccttcggg cgtaccacca cggtccccgg ctccacggac
      721 ggctcgggca cctcatctac cgacatctgc tcgggcccat gccagactat ccccttcaag
      781 ttctcaacct tcccgtagcg aacctcctgc gccaactatc ttactcctaa gatcgcagcc
      841 gtgtggtccc tcccccgtcc ccttgacctt atcccagggt caacccttac actcccttca
      901 gatatggatg ctgaagggcc aagagagcgg cttttgatcg gggaggtcgc cgggctgctc
      961 ggcgtcaccc cgaaggccat caggcactat gaaaagctgg gtctgatcga gaacccggag
     1021 cgctcggaat ccggttaccg cctctacacg gcagacggcc tcttgcggtt gcagcagata
     1081 aagaggttgc gttctcttgg cttctcgctg gagcgcatca agagagtcct cggcgagagc
     1141 ggtcccgggg tcgaactcgg gagcgtgcta gaggcgctcc tcgtcgaggt cgaaagccag
     1201 atcgagcttc tcgaacagag acgggccagc ctgaggcgga tgctctccga gggcgacccc
     1261 tcggaggcgg acgaagaacc ctacatattg gagctcgccc ggcagcactt tggagaacgc
     1321 ctgggcgagc tcgacccgga agtgttggat caggagaaga ggttctgggc gacgctcgac
     1381 gcttttcggt ggcctcgaga attcaggtcg tttcaagaaa ccctcgtgct ctacctcgca
     1441 gaccacccgg agcaatacga acagttgctc atcctcgagc agcggttcgc gacgctggcg
     1501 catcggcccg agaacgcccg agaggtagag caactcgcgg aagaatacgc cgcgtacttc
     1561 gaggcgaacc cgctaccggg ggaactttcg aagggggctg actggggatc cggacccatg
     1621 gaggccgcgc tctcgggggt cgtcctgagc gccatgtctc ccgcccagaa gaggtgtatg
     1681 caactgctcc aggagcacgt cttggggaac gaaggagaac gatgaaacgc gctctgaagg
     1741 tcctcgtgcc cctggtggtc ctggccgaag taattctagt ctggtcgggg gttatggacc
     1801 tcggcgacgc ggtgatcctg gtggctggcc tggaggcgct gctcttcgtg gccgggctgg
     1861 gaggtctcgt actcgtcgtg ggacgttacc ggaggggacg gcgggcagga atggacgcgt
     1921 ggagggctct cgaggacggc ctctccctcg tgctccctcg ggcggtggca cgcctcgtcg
     1981 tcaaggagcc tcgccttctc gtctgcctct tccgttggac catctggcgg gtgaggctca
     2041 cggacgagga gttcgcgtac cacaaacggt ccctgctggg cgctatcgtg ccgctgttgg
     2101 tagtctctgc tcccgtcgag ctgatcctcg ttcacatgct cgccctggcc ttctccccct
     2161 ggtggtggtt gaagtgggcg ctgctcgtcc tgggagcgta cgcgaccctc tggctgctcg
     2221 gcctttacgc ttccctcgtc acgctccctc accggctcga ggaagagggc ctgcggattc
     2281 gctacggcct gctggcggaa gggttcttcc cctatcagga gatcgaggcc acctcgcgca
     2341 gggacctcga ggctcccacc cctggagacg gcctttcgca cgactccggc gaagaaaccc
     2401 tctacctggc ctcgggcggc aagacggacg tttccctacg tctccgggtc ccgcggtcga
     2461 tgagcgggtt ttcgaaggac accgggccgg ctctctgcgt ttgcctggcg gtagacgagc
     2521 ccgacaggtt cgtgcgagaa ctccggctac gtttcggggc cccgacccgg aacccaccat
     2581 ccgttcttgg cgaacggggc accgaa
//
LOCUS       DBNGVD010000512         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3408937, whole genome shotgun sequence.
ACCESSION   DBNGVD010000512 DBNGVD010000000
VERSION     DBNGVD010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3408937"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC16CM_13865"
     CDS             complement(<1..567)
                     /locus_tag="AC16CM_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3140967.1"
                     /translation="MAKINRLRLLAAASAVAVAASVLLLLTALQPVQAAFPGTNGSIV
                     FERDPDGYRGPQDSEIYSINFEGNNLKHLTRNDTEDTRPAWSADGKKLVYSGGGRRSS
                     YSADLFVMKGDGSTKTRITREGEIRGAAKANDLDPAFSPNGRSIAFVRNGPLPEGYDS
                     NNDIYKIGVDGNGLTRIVDIPSFEYRSGG"
     gene            complement(827..>1689)
                     /locus_tag="AC16CM_13870"
     CDS             complement(827..>1689)
                     /locus_tag="AC16CM_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140968.1"
                     /translation="LCWSLWLFWWVRGHHREGRGLAETVLTRDLPYPLKARVSLTVAT
                     MCYTLADYPAARGNWEGALRYSQRAGDALAEAYSWAGTGLVELVRPDFEEAARRMEKA
                     LVLFEGAEEDAVASLTRTWLGTTLLARGNLAGAERRFGEGLDSARRRQDPLCTYVALY
                     NLAQLALVREDLGRAASTLEEGIRLSGWTRDRANLAHFLEALAAVSSQRHDPRRAAVL
                     LGAAETSLREVGAFVYNFYVTDPTLRERTVSEAREALGEVAFGEARAEGEAMTFEDAV
                     GYAIGGDGQT"
BASE COUNT          270 a          568 c          541 g          310 t
ORIGIN      
        1 ccccccggag cggtactcga agctgggtat gtcgacgatc cgggtcaggc cgtttccgtc
       61 gaccccgatc ttgtagatat cgttgttgga gtcgtagccc tcggggagcg ggccgttcct
      121 gacgaaggcg atgctgcgac cgttgggcga gaaggcgggg tcgagatcgt tggccttcgc
      181 cgcaccgcgt atctcgccct ccctggtgat gcgggtcttg gtggagccgt cgcccttcat
      241 aacgaacagg tcggcgctgt aggagctcct tctgccgccg ccggagtaga ctagcttctt
      301 gccgtccgcc gaccacgcgg gtcgcgtatc ctccgtgtcg tttctggtga ggtgcttcag
      361 gttattgccc tcgaagttta tggagtagat ctccgagtct tgtgggcccc gatagccgtc
      421 ggggtccctc tcgaagacta tgctgccgtt cgttccgggg aacgccgcct ggaccggttg
      481 cagggctgtg agcagtaaca ggacgctcgc ggccacggcg acggccgacg cggcggcgag
      541 aagcctgagg cggttgatct ttgccatctg gacacttcct cctcttttgt ggttgttctg
      601 ggtcatccga acgcctccct actatgacgt ccagactacg aaccggccac ggacaccgcc
      661 cacacaccac ccacacaacc aggaccgtgt gatgcggtgg cgaaaaacct cgctgcgaat
      721 gactggcgtt gaccgagagc ccgggagggc tcacgatcct tggctctggt tctggggtgg
      781 ttgtcggagg acccgctcgg ggaccctcgc cgcgcagggg gtttccctag gtttggccgt
      841 ctcccccgat agcgtacccg accgcgtctt cgaaggtcat ggcttcccct tcggcgcgcg
      901 cttccccgaa ggcaacttcg ccaagggcct cacgtgcctc ggacaccgtg cgttcccgga
      961 gggtgggatc cgtcacgtaa aagttgtaga cgaaagcccc aacctcccgc agcgaggtct
     1021 cggccgcgcc gagcaagacc gcggcgcggc gcgggtcgtg tcgctgggag gacaccgccg
     1081 ccagggcctc cagaaagtgt gccaggttcg cccggtccct cgtccaaccg gagagtcgta
     1141 tcccctcctc cagggtactc gcagcacgcc cgagatcttc cctgacgagc gccagttgcg
     1201 ccaggttgta gagtgccacg taggtgcaga ggggatcctg ccttcgcctg gcggagtcaa
     1261 gcccttcccc aaaacgccgt tcggccccgg caaggttccc gcgcgcgagc agcgtcgtgc
     1321 ccagccaggt acgggtgagc gaagctacgg cgtcctcctc ggccccctcg aagaggacga
     1381 gggccttctc catgcgccgg gccgcctcct cgaaatcggg acggacgagc tccaccagcc
     1441 ctgtgccggc ccaggagtag gcttccgcca gcgcatctcc cgcccgctga gagtagcgca
     1501 gggcaccttc ccagttccct ctggcggccg ggtagtcggc cagcgtgtaa cacatcgtgg
     1561 ccaccgtcag agacaccctg gctttcagag gataggggag atcacgtgtg agcacggttt
     1621 ccgccaggcc ccggccctcc ctgtggtggc cacgcaccca ccagaatagc cagagcgacc
     1681 agcacaggc
//
LOCUS       DBNGVD010000513         6786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4138743, whole genome shotgun sequence.
ACCESSION   DBNGVD010000513 DBNGVD010000000
VERSION     DBNGVD010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4138743"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="AC16CM_13875"
     CDS             complement(<1..801)
                     /locus_tag="AC16CM_13875"
                     /inference="COORDINATES: protein motif:HMM:NF046887.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 2 galactose-binding
                     domain-containing protein"
                     /protein_id="HIR3140969.1"
                     /translation="MFEQWKLQDYAPGEGSASGAFEEGLDDTGWVDVHVPGDVHSALT
                     SAGHIEDPFYDRNENECAWIENREWWYRTTFQGPEEPLQCDERLRLVFHGLDTFATVW
                     MNGEELGGHQNMFREATFDVGASVRPGENTLAVLFDRPLDHVGEEHPDQWGRNPERVS
                     MRKAQFGFGWDWGPRLPTVGIWRPVGLRRERRARIKGVHFHTLEIAPTGGRAAVAVRV
                     EADRFAAEDAIETSISLTGPDGEPVSEATLTLEGEGADLTGTAYVTVER"
     gene            complement(900..1328)
                     /locus_tag="AC16CM_13880"
     CDS             complement(900..1328)
                     /locus_tag="AC16CM_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3140970.1"
                     /translation="MITDLGHPAFAAHDVDQALRFYEIFGVEEAFRLHHDDGSLMLVY
                     LHVSGDRFIEVFPGGPAPDPDRVQSFKHICLLTDDIHEAVGRLRENDAPVDSEPLLGL
                     DGNLQAWTHDPDGNQIELMQLAEDAPQRLAARSATGDDPR"
     gene            1493..2869
                     /locus_tag="AC16CM_13885"
     CDS             1493..2869
                     /locus_tag="AC16CM_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIR3140971.1"
                     /translation="MSSSTGSAGDGTNKFVYVVAAVAAMGGLLFGYDTGIISGALLFI
                     ADDFKLSDTAQQIVVASLLLGAVFGALFGGPLADRIGRRRAIMTAAVIFVVGSLASAL
                     ATGAAFLAIARFVLGLAVGGAGMVVPVYIAESSPSRVRGSLVSLQQFLITVGILLSYG
                     INFLLADAEAWRLMLGLGVVPALALLVGMFFLPESPRWLVGMDRDDEARSVLARTREG
                     DEAVEEEMKAISGADHAEERTSYRDLLKRRYRPALTVGVGVAFINQMVGVNAVIYYAP
                     SILSDAGFGDSGAILATTGIGLVNCLVTGLALLSIDRVGRRPLLLVGTGGVTISLIVL
                     GLAYLLPPQTALVNVILVGGLMVYIASFAASLGICIWLLNSEVYPLEIRGKGSATGSI
                     THWVLDLIIASTVLTLINTITETGTFWLYGAFGIIGLIFFFRVVPETKGRSLEDIEEE
                     LEKRVSTS"
     gene            3167..3388
                     /locus_tag="AC16CM_13890"
     CDS             3167..3388
                     /locus_tag="AC16CM_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140972.1"
                     /translation="MNDKIYLKGGSDQVRADKYRADRDLIYGGKGYDLIYVNDGDTHD
                     RVRGGAGNDKCYVDSRVEVVSGCARVITP"
     gene            complement(3541..4593)
                     /locus_tag="AC16CM_13895"
     CDS             complement(3541..4593)
                     /locus_tag="AC16CM_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR3140973.1"
                     /translation="MITGPQGDPLARRDLLRRIGRLEQVAGIEPFVLDDGPSRGVRAL
                     RFRTGGGLSFDVLPDRGMDLGFAEYGGTPLAWLSPNGIVAPSFHEPGGEGWLNSFPGG
                     LLVTCGLRNVGPPGERGGEEFGLHGRASHIPATRVSYDTVWDDGGCVLEASGQIRESR
                     VFGPNLVLRRRITARVGESRLRIEDSIENEGFDPELLMVLYHLNLGWPLLSEASHLTG
                     PGGPGEPPEPRDEEARAGLEAWDRFEPPTPSFKERVFYHHPTAGPDGRSRARLDNPEP
                     GLSFSVTFDPRELPEFIQWTMTGEGTYVLGLEPATCRVEGPAAEEAAGRVIRLQPGET
                     RQHILELKVSRVSASG"
     gene            4681..5754
                     /locus_tag="AC16CM_13900"
     CDS             4681..5754
                     /locus_tag="AC16CM_13900"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HIR3140974.1"
                     /translation="MEAYPIELTYHVRDYYFGERLIPELLGKRDTPDGKVSETWEISD
                     YRETVGTVVNGPYAGRTLHEITEEFPDELVGEGWRGPHFPLLIKFLDASHLLPVHLHA
                     DDETARTKHGEPHGKAEAWHILWAADDATILAGIKEGRSREDLISAFKTQDYEAVMPR
                     HGVASGDTVYVPGGILHTFGPDTMILEVQQTSDLGQFVMPFDLYGNRLDEEEWDANIE
                     ATLDELKRDFMPRPNPGLVLEDGVNRRVLCCAGPNFALERWTLAEPHTEPAHPRRCFT
                     LSNVGEAPVTLEYEGGTQTLGRAESRVIPAAIGEVRVVPGETGETSLVACYVPDLELD
                     VVGPSRDAGYSDEEIKALGEVDI"
     gene            5882..>6786
                     /locus_tag="AC16CM_13905"
     CDS             5882..>6786
                     /locus_tag="AC16CM_13905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3140975.1"
                     /translation="MGKIVTLGEVVSDIYRGEEFSEVEIGFVARPGGAPANVAVAASR
                     LGSEATFVGSVGQDLFGDFILRALEAEGVDTSHVGRQRQPTRTSLAFVEIGTDGDREF
                     TFYRSSPAADELLSPEDVTEEVLSGASFVNFGSIPLIREPVRSATHRIAELARKHDVP
                     VAFDVNFREHLWESTSAAREAVDPLLELSDIVKLGDDELPPLLDTEDVEEAAQALLDR
                     GVTLVLISKGPDGAFYATSEYSGEVPTYDVGDIVDATGAGDAFLAASLVHLSDHPFDE
                     ERVREASLRGCAAGALACTDYGAMRA"
BASE COUNT         1141 a         2173 c         2234 g         1238 t
ORIGIN      
        1 tctctccacg gtaacgtagg ccgtgcccgt caggtcggcg ccttctcctt cgagcgtgag
       61 cgtcgcctca gagacaggct ccccgtcggg cccggtcagc gagatggagg tctcgatcgc
      121 gtcctccgcc gcgaacctgt cggcctctac ccgcacggcg acggcggccc gccctcccgt
      181 cggggcgatc tccagggtat ggaagtgaac tcccttgatg cgggcgcgcc gttcccgccg
      241 cagaccgacg ggacgccaga tcccgacggt cgggagcctc ggcccccaat cccacccgaa
      301 gccgaactgc gccttgcgca tggagacccg ctccgggttc ctgccccact gatcggggtg
      361 ctcctccccg acgtggtcga gcggcctgtc gaacagaacg gcaagcgtgt tctcgccagg
      421 acgcaccgac gcgccgacgt cgaaggtggc ctcccggaac atgttctggt gcccgccaag
      481 ctcctcgccg ttcatccaga cggtggcgaa ggtgtcgagg ccgtggaaga cgaggcgaag
      541 ccgctcgtca cactgcagcg gctcctctgg gccctggaag gtcgttctgt accaccactc
      601 gcggttctcg atccaggcac actcgttctc gttgcggtcg tagaagggat cctcgatgtg
      661 tccggcggag gtgagggctg aatggacgtc cccgggaacg tgtacgtcga cccaccccgt
      721 atcgtcgaga ccttcctcga aggcgccaga agcgctgcct tctcccgggg catagtcctg
      781 tagcttccat tgttcgaaca tcggatggtt ccccctcctg gagctggatt ctactgcctc
      841 gagccgccgg cggtgaaccc cggatcaggc atctctgcca ggccggacct gacggaccgc
      901 taccgtggat cgtctcctgt ggcggagcgt gcggcgagcc gttgcggggc gtcttcggcg
      961 agttgcatca gctcgatctg gtttccgtcc gggtcgtgtg tccaggcctg gaggttgccg
     1021 tcgaggccga gtagaggctc gctgtctacg ggcgcatcgt tctcgcgcag gcgtccgacg
     1081 gcctcgtgta tatcgtcggt caggaggcag atgtgcttga agctctgcac ccggtcgggg
     1141 tcgggggccg gcccgccggg gaagacctcg atgaagcggt cgcccgagac gtgaaggtaa
     1201 acgagcatca gcgaaccgtc gtcgtgatgc agccggaaag cctcctcgac gccgaatatc
     1261 tcgtagaacc gtagggcctg gtcgacgtcg tgtgccgcaa aggcggggtg tccgaggtct
     1321 gtgatcacgg gtcaaactcc tctcgttgcc ggtgccgggt gggtgacggg acagggtgtc
     1381 tttccgcggt attgctggct tcacccgttt gtgcttcgtc ctcgcgggta aagatcccct
     1441 gtgatattac cgttcgacgc accgaacggc agggaggaaa gggaggaata tcatgtcttc
     1501 gagtaccggc tcagctggcg atggcaccaa caagtttgtg tatgtggtag ccgccgttgc
     1561 cgccatgggt ggtctgttgt ttggttacga caccggaatc atctccggcg cgttgttgtt
     1621 catcgcggac gacttcaagc tttcggacac ggcgcagcag atcgtggtcg catccctcct
     1681 gttgggggcc gtgttcgggg ccctgttcgg cgggccgttg gcggaccgga tcggacgccg
     1741 gcgcgcgatt atgactgccg ccgtcatctt cgtggtcggg tcgctggcct cggcgctggc
     1801 gaccggcgcc gcgttcctgg ccatcgcgcg cttcgtgctc gggctggccg tcggcggcgc
     1861 ggggatggtg gtcccggtct acattgcaga gtcctccccg agccgggtgc gcggctcgct
     1921 cgtctccttg cagcagttcc tgatcaccgt aggcatcctg ctctcctacg gcatcaactt
     1981 cctgctcgcc gatgccgaag cctggcgcct tatgctcggc ctcggcgtgg ttccggcact
     2041 cgccctcctg gtcggcatgt tcttcctgcc cgagagcccc cgctggctgg tcgggatgga
     2101 ccgggacgac gaggcccgct ccgtgctcgc ccgcacccgt gagggagacg aagccgtgga
     2161 ggaggagatg aaggccatct caggggccga tcacgccgag gaacgcacct cctaccgtga
     2221 cctcctgaag cgccgctaca ggcccgcgct cacggtcggg gtcggggtcg cttttatcaa
     2281 ccagatggtc ggcgtgaacg ccgtgatcta ctacgccccg agcatcctct ccgatgccgg
     2341 cttcggggat tcgggtgcta tcctggccac caccggcatt ggccttgtca actgcctggt
     2401 cacgggcctt gccctgctct ccatcgaccg cgtgggacgc aggcctctcc ttctcgttgg
     2461 caccgggggc gtgacgatca gcctgatcgt tctcggcctg gcctacctcc tgccgcccca
     2521 gaccgccctg gtgaacgtga tcctcgtcgg cggccttatg gtctacatcg cctcgttcgc
     2581 cgcctcgctc ggcatctgca tctggctgct caactccgag gtctaccccc tggagatccg
     2641 cggcaagggc tcggccaccg gcagcattac ccactgggtg cttgacctca tcatagcctc
     2701 cacggtactg accctgatca acaccatcac cgagacgggc accttctggt tgtacggcgc
     2761 cttcggcata atcggcctga tcttcttctt ccgcgttgtc ccagagacga agggacgctc
     2821 gctcgaagat atagaagagg agctggaaaa gagggtctcc acgagctagc acgccagtca
     2881 ctccggtctc tgcctgctgg cgcgacagtg tagcgctgcc agatgggagt tgcgcgaaaa
     2941 ttggcactgg tgtaaggtgc aaatcgccgt ggaatggggc cgctctggtc ccgagaacag
     3001 gaggacgtgg atgcgcaagc tagcgatgat ggccgctgtg gtgatggcgc tttcggtgtt
     3061 tgttgcaact gcagcattgg ccggaaatca ggtaatccag tgccagggca ttccctgcat
     3121 tgcgaccggt agccaggacc tcgtcaaaga gagaatcggc aacaggttga acgacaagat
     3181 atacctcaag ggcggtagcg accaggtacg cgccgacaag tacagggccg atcgggacct
     3241 catctacggt ggcaagggct acgacctgat ctacgtgaac gacggcgaca cccatgacag
     3301 ggtccgcggt ggggcgggca acgacaagtg ctacgtggat tccagggtcg aagtggtcag
     3361 cggctgcgcc agggtaatta ccccgtagag gtcgcatctg ggaaccagcc ccacttgggc
     3421 aggcgcacag cataaccagc atggaggggc cagggcttcg aagccctggc ccctctctcg
     3481 tgccagggat gcgcaggatg cagccccgag ctcgaatggc aatcgagatg atccaggttc
     3541 tcagccagaa gcactcaccc gggacacttt gagctccagg atatgctgtc gtgtttcgcc
     3601 aggttgcagg cggataacgc ggcctgcggc ctcttctgcc gccggaccct cgaccctgca
     3661 ggtggccggt tcgagcccga gtacgtaggt gccttcgccg gtcatcgtcc actggatgaa
     3721 ctcgggaagc tcccggggat cgaacgtcac ggagaacgag agcccgggct cggggttatc
     3781 caggcgtgcc cgcgaccggc cgtccggtcc cgcggtggga tgatggtaga agaccctttc
     3841 cttgaagctc ggggtcgggg gctcgaatcg gtcccaggct tcgagacctg ctctcgcctc
     3901 ttcgtccctc ggctcgggtg gctctcccgg tcctccaggg ccggtcaggt gggaggcttc
     3961 ggagagcagg ggccagccga ggttcaggtg gtagaggacc atgagtagtt ctgggtcgaa
     4021 gccttcgttt tcgatgctat cctcgatcct caggcgtgac tcgccgacac gggccgtgat
     4081 gcggcggcgc agcacgaggt tcgggccgaa gaccctcgac tccctgatct ggccgcttgc
     4141 ctcgagcacg cagcccccat cgtcccacac tgtgtcgtag gagaccctgg tggcggggat
     4201 gtgcgaggcg cggccgtgca gaccgaattc ctcgcccccg cgctctcccg gtggtcccac
     4261 gttcctgagg ccgcaggtga ccaggagacc gccggggaaa gagttcagcc agccctcgcc
     4321 ccctggctcg tggaaggaag gggcgacgat gccgttgggg gagagccagg ccagcggcgt
     4381 gcccccgtac tcggcgaagc ccaggtccat gccgcggtcg ggaagcacgt cgaaggagag
     4441 gccgcctccg gtcctgaagc ggagggcccg cacaccgcgg gacggcccat cgtctaagac
     4501 gaagggttcg atcccggcaa cctgctccag ccttcctatc cgcctcagga gatcccttcg
     4561 cgccagcggg tctccctgtg ggcctgtgat catagcgctc ctccttcccg gtccgagccg
     4621 gggaatgcca acgtgggtag aatacccgtg tcggagagct aggggatagg gggaggggct
     4681 ttggaagcct acccgataga gcttacatac cacgtgcgcg actactattt cggggaacgc
     4741 ctgatccccg agttgttggg caagagggat acgccggatg gcaaggtgtc ggagacctgg
     4801 gagatcagcg actacaggga gaccgtgggg acggtcgtca acggaccata cgcgggccgt
     4861 acgctgcacg agataaccga ggagttcccg gacgagctgg tgggcgaggg ctggcgcggg
     4921 ccgcatttcc cgctcctcat aaagtttctc gacgcctctc acctgctccc ggttcacctg
     4981 cacgccgacg atgagaccgc caggaccaag cacggcgaac cgcacggcaa ggccgaggcg
     5041 tggcacatcc tgtgggccgc agacgacgcc acgatactgg ccggcataaa ggaaggccga
     5101 tcgcgcgagg acctgatcag cgcgttcaag acccaggatt acgaggctgt tatgccgagg
     5161 cacggtgttg cttcagggga caccgtctac gtgcctggcg gcatcctgca cactttcggg
     5221 ccggatacca tgatcctgga ggttcagcag acctccgacc tgggccagtt cgtgatgccc
     5281 ttcgacctct acggcaaccg cctggacgag gaggagtggg acgccaacat cgaggccacc
     5341 ctcgacgagt tgaagaggga cttcatgccg cgtcccaacc cggggctggt tctcgaagac
     5401 ggcgtcaacc ggcgcgtgct gtgctgcgcg ggtccgaact tcgcgctgga acgctggacg
     5461 ctggcggagc cgcacaccga gccggcgcac ccgcggcgct gcttcacgct ctccaacgtg
     5521 ggcgaagccc cggtgacgct ggagtatgaa ggcggcacac agacgctcgg gcgcgccgag
     5581 agccgcgtga tacccgccgc aattggcgag gtgagggtgg ttccgggcga gacgggcgag
     5641 accagcctcg tggcctgtta cgtccctgac ctcgagctcg acgtcgtagg accatcacgt
     5701 gatgccgggt actcggacga ggagatcaag gccctgggcg aagtggacat ctagggcggt
     5761 tgcctagatc gtgaacccgg ggggagcaaa ggcaacaggc atcttcctga agcctgttgc
     5821 cttccctggc gtggctcacc atcatcaagg atcaacatag cgttaccaag gggaggaatt
     5881 tgtgggaaag atagtcactc tgggcgaggt cgtctcggat atctacaggg gcgaggagtt
     5941 ctcggaggtg gagatcgggt tcgtggccag gcccgggggg gcgccggcga acgtggccgt
     6001 ggccgcctcc cggctcggct ctgaggccac cttcgtgggg agcgtggggc aggatctctt
     6061 cggggacttc atcctgcgcg cgctggaggc cgagggcgtg gatacctccc acgttggccg
     6121 ccagcgtcag cccacccgta cctcgctcgc cttcgtcgag ataggcaccg acggcgaccg
     6181 cgagttcacc ttctaccggt ccagcccggc cgcggatgag ctgctctcgc ccgaggatgt
     6241 taccgaggaa gttctctccg gggcctcctt cgtcaacttc ggaagcattc cccttataag
     6301 ggaaccggtc cgctcagcca cccaccgaat cgccgagctc gcccgcaaac acgacgtccc
     6361 cgtggctttc gacgtcaact tccgcgagca cctctgggag agtaccagtg ccgccaggga
     6421 ggccgttgac cccctcctcg aactttcaga tatcgtgaag cttggcgatg atgagctacc
     6481 acccctactc gatacggagg acgtcgaaga ggccgcccag gcgttgctcg accgtggcgt
     6541 aaccctggtc ctcataagca agggaccgga cggcgccttc tacgccacca gcgagtacag
     6601 cggcgaggtt ccaacctacg atgtgggcga tatagtagat gccaccggcg ccggtgacgc
     6661 gttcctggcc gcctccctcg tacacctctc tgaccatcct ttcgacgaag aacgggtgcg
     6721 cgaggcctcc cttagaggct gcgccgcagg agccctggcc tgcaccgact acggcgccat
     6781 gcgcgc
//
LOCUS       DBNGVD010000514         2194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4685833, whole genome shotgun sequence.
ACCESSION   DBNGVD010000514 DBNGVD010000000
VERSION     DBNGVD010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4685833"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="AC16CM_13910"
     CDS             complement(<1..157)
                     /locus_tag="AC16CM_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140976.1"
                     /translation="MEDRREQQGRGELQHSLKRRHMSMIALGGVIGAGLFVGSGVVMN
                     QTGPAAIL"
     gene            complement(231..1625)
                     /locus_tag="AC16CM_13915"
     CDS             complement(231..1625)
                     /locus_tag="AC16CM_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019720091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR3140977.1"
                     /translation="MTQDTKDLLAKDRRYVWHAMTGHNPGATTMVVTEADGAWVTDVD
                     GNRYLDGMAGQWSVNAGYGREELARAAYEQLSTMPFYPMVQGHLPAIELAERLNGWLD
                     DDYVFFYSNSGSEANEAAFKIARQYHQQNGEGSRWKFVARYRGYHGNTLGALAATGHA
                     QRKYHYEPLAPGFLHVSPPDGYRCIYCDGGGECGRECALKYAKEIQRVIEWELPETVA
                     GVIMEPIITGGGIIIPPEGYVEEVARVCEETGVLLIVDEVICGFGRTGTMFGHQHYGI
                     RPDIVTMAKGITSSYFPLSATAVKRTLFEKFAGTEEYGRLRHINTFGGHPGACAVAIR
                     NLQIFEEENLVGRSAAMGERLRDGLSPLENHPHVGQVRGRGLLLGIEVVEDRETREPA
                     SAQRMGEVISGCKELGLIVGKNGDTTPGFNNVITLCPPLNITDDDLKFIVETLQTVFE
                     GSANGDRPVRPPIA"
     gene            complement(1749..2144)
                     /locus_tag="AC16CM_13920"
     CDS             complement(1749..2144)
                     /locus_tag="AC16CM_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR3140978.1"
                     /translation="MFRATGSIVEGEALVSAEGFSPRYDLDRSSGEISRPGHAIAGEN
                     IAGKILFFPTAKGGIAAGWAFYDLASKAIAPKAIVFGKTNPVMVQGAVFAGITITEGW
                     SENPTEVFRTGDTVRVDPLRKTVELLKGA"
BASE COUNT          336 a          776 c          661 g          421 t
ORIGIN      
        1 agaggatcgc cgcaggcccc gtctggttca tcaccacgcc gctgccgacg aagaggccgg
       61 ccccgatgac ccccccgagc gcgatcatcg acatgtgcct gcgcttcaaa ctgtgctgca
      121 gctcacctcg cccctgctgt tcccgccgat cttccatgcc acctcctcac cactatttgt
      181 tcatttattt tgtgaacatc gtggaaccct accgattcgg aaaaccgggg tcaagctatc
      241 gggggcctca ccggcctgtc gccgttggcc gatccctcga agaccgtctg cagggtttcc
      301 acgatgaact tcaggtcatc gtcggtgatg ttcagcggtg ggcacagcgt aatgacgttg
      361 ttgaagcccg gcgtggtgtc gccgttcttg cccacgatga ggcctagctc cttgcagccg
      421 gagatgacct cgcccatcct ctgggcgctg gcgggctcac gcgtctccct gtcttcgacc
      481 acctcgatcc cgagcaggag tccccttccc ctgacctgcc cgacgtgcgg gtggttttcg
      541 aggggggaga ggccgtctcg cagtctctct cccatggccg cggagcgccc tacaaggttc
      601 tcctcctcga agatctgcag gttccttatg gcgacggcgc aggcgcccgg gtgtccgccg
      661 aaggtgttga tgtggcgcag gcgaccatac tcctccgttc cggcgaactt ctcgaagagc
      721 gtgcgtttca cggccgtggc cgagagcggg aagtaggagc tcgttatgcc cttggccatc
      781 gtcacgatgt cgggtcttat cccgtagtgc tggtggccga acatcgtgcc cgtcctgccg
      841 aaaccgcaga tcacctcgtc cacgatcagg agcacgccgg tctcttcgca gaccctggcg
      901 acctcctcca cgtagccctc cggcggaatg atgatgcccc cgcccgtgat gatgggctcc
      961 atgatcacgc cggccaccgt ctcgggaagc tcccactcta tgacgcgttg gatctccttc
     1021 gcgtacttga gggcgcactc cctgccgcac tccccgccgc catcgcagta aatgcaacgg
     1081 tagccgtccg gcggagagac gtgcaggaac cccggtgcca gcggctcgta gtggtacttg
     1141 cgctgcgcgt ggcccgtggc cgcgagcgca cccagcgtgt tgccgtggta gcccctgtag
     1201 cgcgcgacga acttccaccg gctcccttcg ccgttctgct ggtggtactg gcgcgcgatc
     1261 ttgaacgccg cctcgttggc ctcagacccc gagttggagt agaagaacac gtagtcgtcg
     1321 tccagccatc cgttcaaccg ctcggccagc tcgatggccg gcaggtgccc ctgcaccatg
     1381 gggtagaagg gcatggtgct gagctgctcg taggcggccc gcgccaactc ttcgcggccg
     1441 taacccgcgt tcaccgacca ctggccggcc atcccgtcga ggtagcggtt gccgtccacg
     1501 tcggtcaccc aggccccgtc agcctccgtc accaccatcg tggtcgcccc cgggttgtgg
     1561 ccggtcatgg cgtgccagac gtagcgccga tccttcgcca gcaagtcctt ggtatcctgc
     1621 gtcattcgtg cctcccgtca gggtgtgcgc ccctacctac cgaagccggc gcgcgaccct
     1681 ccaggcgccg accggcccgt atcatcaacc acctgcaaag acgacgtcaa gcagaggccg
     1741 gcacccgctc acgccccctt caggagctcg acggtcttcc tgagcgggtc gaccctcacc
     1801 gtgtcgccgg tccggaagac ctcggtgggg ttctccgacc agccctcggt tatcgtgatc
     1861 ccggcgaaga cggcgccctg caccatcacg gggttggtct tgccgaacac gatggccttc
     1921 ggggctatgg ccttgctggc caggtcgtag aaggcccagc ccgccgcgat accgcccttg
     1981 gcggtcggga agaagagtat cttgccggcg atgttctcgc ccgctatggc gtggccgggg
     2041 cgcgagatct ccccgctcga acggtccagg tcgtagcggg gactgaagcc ttcggcggag
     2101 accagggcct caccttccac gatgctaccg gtggcccgga acacggatat gccgtttctg
     2161 gcgctcaaga ccccccgatc ctgcccgtca cggc
//
LOCUS       DBNGVD010000515         4299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4502610, whole genome shotgun sequence.
ACCESSION   DBNGVD010000515 DBNGVD010000000
VERSION     DBNGVD010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4502610"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..466
                     /locus_tag="AC16CM_13925"
     CDS             119..466
                     /locus_tag="AC16CM_13925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR3140979.1"
                     /translation="MTTVRSDGQPQTVPVWFLWEYEGFLIYSQPDRQKLRNISRNPRV
                     GLNLNSDAQGGNVVRLEGKATIVDDAPPSGEVPSYVEKYREGISRIGFDVDGFARAYS
                     VAVRVKPDRWQVW"
     gene            complement(560..1885)
                     /locus_tag="AC16CM_13930"
                     /pseudo
     CDS             complement(560..1885)
                     /locus_tag="AC16CM_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
     gene            complement(2011..2835)
                     /locus_tag="AC16CM_13935"
     CDS             complement(2011..2835)
                     /locus_tag="AC16CM_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3140980.1"
                     /translation="MTLTIGTGPFGDQGEKAFNFEAGPPGDHVLYFEDSPRRVRAVLG
                     GETVADSRRVKLMHETGYLPIYYFPEEDVRMDLLEATDHTSTCRFKGEASYWSVRVGE
                     TVAENAVWSYPDPIESAPPIAGYLAFYWRKMDRWFEEDEEVFVHPRDPYHRVDALQSS
                     RHVKVSVNGEVVAETGRPVVVFETGLPPRYYIPPDDVREDVLVPSDHRTQCPYKGTAS
                     YHSVEAGGETVENVVWHYQEPIAAAEKIRGTLSFFNEKVDLEVDGEPQERPRTQWS"
     gene            3056..3865
                     /locus_tag="AC16CM_13940"
     CDS             3056..3865
                     /locus_tag="AC16CM_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3140981.1"
                     /translation="MHRKTGTAEVNGARIHYEGAGEGEPLVLVHAGIADSRMWEAQIP
                     AFAESYRVIRYDLRGFGKSEMVEGPFSHHEDLRGLLDSLGVERAHLVGCSMGGGAVLD
                     FALEYPERVGDLVLVGSAIGGFRPDFGPPREWDELVAADEAGDLERISWLEVAMWVDG
                     PARSPEDVPAPIRDLVYEMNLIALQTEAAELGEEWEPEPPASDRLHDIYAPTLLIVGD
                     EDQPRVFAAADLLERELPNERKVVMHGTAHLPNMERPEEFNRLVLEFLQDH"
     gene            4033..>4299
                     /locus_tag="AC16CM_13945"
     CDS             4033..>4299
                     /locus_tag="AC16CM_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3140982.1"
                     /translation="MISSGELILRLGVAGVLGALVGLERERLEWAAGMRTHALVSLGS
                     ALFMVVSIFGFSDILNERHVVFDPSRVAAQVASGIGFIGAGTIIF"
BASE COUNT          725 a         1348 c         1428 g          798 t
ORIGIN      
        1 agctgaaagc gcggtttgcg ccagcaaacc gcaaagaaag gagcttccct tgttcgacac
       61 ttccaccgac gctggaaggc gggcggagtc acggctagag gaagaggaga tcgcctggtt
      121 gaccaccgta cgctccgacg gccagccgca gaccgtgccc gtgtggtttc tgtgggagta
      181 tgaggggttc ctgatctact cccaacccga ccgccagaag ctaagaaaca tctcccgcaa
      241 ccctcgggtc ggcctgaacc tgaactcgga tgcgcaaggt gggaatgtgg tgcgtctgga
      301 gggtaaggcc acgatagtcg acgacgcccc accttcgggc gaggtccctt cgtacgtcga
      361 gaagtaccgc gagggtatat cccgcatagg cttcgatgtg gatggcttcg cccgggccta
      421 ctcggtggcc gtgcgcgtca aaccggaccg ctggcaggtc tggtgaggag gcatcaggct
      481 gcgggtgaca ggctgatgcc tcctcaccca cagcccgttc tggttctgac tctccggtaa
      541 ccgcctcgcg atcagaggcc ggggatctgg ccgtacggaa ggcgtccacg aagaggctgt
      601 ggtcccggcg gacggtcgcc gagtagcggg ctccgaagtc gaccatctcg gcgacgaagt
      661 cgtcctcgtt gtcgtctatc gccgcgacga tcgcgtcctc cgtctggaag tcgaccaggg
      721 tggggtccga gtcctcgtct gccacgcagt ggatcttggc ggtggcacgg ccgaggtagt
      781 cgagcacggg ggccatctgc tctggctcgg tcaggtccga ccagtcgagg tcctcgacgt
      841 agggggatag ctcggcgacc acgaagccga ccccttctac ctcggtgtag ccgagccatg
      901 ggtcggcgtg ggcctgcagc gcacgctgcg agacggcggt gcggtgcccg ttgtgctcga
      961 agtggcgccc gatgcgctcg tcgccaacga tccggctcgg ggccgcgacg ttgccctgct
     1021 tcatggagag caccacgtcg ttctccaggg cctgggttgg gccctccacc agaacggtgt
     1081 aggccgggag cccggcggag ccgataccga agctctggct gccgacgatg tcctttacct
     1141 ggtaggtgag gctcctggcg cgtttctcct cggggatggt cttcaggtag tcagcgaagg
     1201 ctgcctccac cgtggtgcgc tcctcgtcgt cgaggtcgcg gacccgcgag ctacgccgga
     1261 agcgccgctc gacgttctcg tcaatcaggg tcatcctgtc gagcatgccg acgcgggtgt
     1321 tctccctggt ctcgcgcagg atctcgcgaa tcctgccgtc cgtggtgtcg agccgcagcg
     1381 cgtactcatg gtcgcggtcg ctctcgacga agtagcgtac ctgatccacg taagagcgga
     1441 cgtaggtctg gatgagggag gcgatgtcgt cgtcggagat ggccttggac caggcgatca
     1501 gggcgacgct ggcgaccatc cgcttgatgt cccaggtgaa gtgccccaga taggcctcgt
     1561 cgaagtcgtt gacgtcgaag acgaacaccc cgtctccggc caggtaggtg ccgaagttct
     1621 cagcgtgcag gtcgccctgg atccagaccc ggccggtgcg ctcgttggcc cagcggtcct
     1681 cttcatgtgt cgtgtcggcg tagaacaggc acgcgctgcc ccggtagaag gcgaacgggc
     1741 ctgcggccat cttgcggaac ttctggcgga acgcctcggg gtcggcgtgc atgagctcgg
     1801 agaaggcgtc gacgagcacg tcgacgatct tctcctgacg tccgccagag gactcttgcc
     1861 gcccctgact cgctgtttcc gtcatgaaac tccctcgcgc tcgttgtcag gataccggat
     1921 agcagaattc tattgctttt ttgacgcgca cgagtcgctc gatcggaggc atcgcacaat
     1981 tttctcgaag cctgatgaag cctctgccac ctacgaccat tgcgtccttg ggcgctcctg
     2041 tggctctccg tcgacctcga gatccacctt ctcgttgaag aagctgaggg tgccccgtat
     2101 cttctcggcg gcggctatgg gctcctggta gtgccacacg acgttctcca cggtctcgcc
     2161 gccggcctcg acagagtggt aagacgcggt tcccttgtac gggcattgcg tccggtggtc
     2221 gctcgggacc agaacatcct cgcgcacgtc atcgggcggg atgtagtagc gtggcggtag
     2281 cccggtctcg aacacgacca cgggccgccc cgtctcagcg acgacctcgc cgttcacgct
     2341 gaccttgacg tgcctggagc tctgtagcgc gtccaccctg tggtacggat ccctgggatg
     2401 gacgaagacc tcctcgtcct cctcgaacca ccgatccatc ttccgccagt agaacgcgag
     2461 gtaacccgcg atcgggggcg ccgactcgat cgggtccggg tacgaccaca cggcgttctc
     2521 ggccacggtc tcaccaaccc gcaccgacca gtaggaggcc tcacccttga agcggcacgt
     2581 cgacgtatga tccgtggcct ctaggaggtc catccgcacg tcttcctctg gaaaatagta
     2641 gatgggcagg taccccgtct cgtgcatcag cttgacgcgg cggctgtcgg ccacggtctc
     2701 gccgccaagc accgccctca cccggcgtgg ggaatcctcg aagtacagta cgtggtcccc
     2761 ggggggacct gcctcgaagt tgaacgcctt ttcaccctgg tcgccgaacg gtccggtccc
     2821 gatggtcaac gtcatggcat gctcctcggt cggcctgcta ccaccgcatg ttatacaccg
     2881 cagcccccag ggatcatcct gcgccaaccg tgccaggggc gtatactgag ccggccccat
     2941 cagggcaacc ggtgcgtctt cacagagcgg gcacgaccga acagggagag accgggtgcc
     3001 ggcctgccgc gcccgatatc gcagacgatg tgtacccggc ggggaagggg caaagatgca
     3061 tcgaaagacc ggtacggccg aggtcaacgg agcgcggatc cactacgagg gcgcggggga
     3121 gggcgagccg ctcgtcctcg tgcacgcggg gatcgcggac agcaggatgt gggaggccca
     3181 gatcccggcc ttcgccgaaa gctacagggt tatacgctac gacctgcgcg gcttcgggaa
     3241 atccgagatg gtcgagggac ctttctccca ccacgaggac ctgcgcggcc tgctcgactc
     3301 gctgggcgtg gagcgggcgc acctcgtggg ctgctcgatg ggtggcggtg ccgtgctgga
     3361 cttcgcgctc gagtacccgg agagggtcgg agacctcgtt ctggttggtt ccgccatagg
     3421 cgggttcagg ccagacttcg gcccccccag ggagtgggac gagctcgtcg ccgccgacga
     3481 ggccggggac ctggagcgga tctcctggct ggaggtcgcg atgtgggtcg acggccccgc
     3541 aagatccccc gaagacgtcc cggctccaat ccgcgacctc gtctacgaaa tgaacctgat
     3601 cgccctccag accgaggccg cggaactggg cgaggagtgg gagccagagc cgcccgcctc
     3661 agaccgcctc cacgacatct acgccccaac actcctgata gtcggtgatg aagaccagcc
     3721 cagggtcttc gccgcagccg acctcctcga aagggagctc cccaacgagc gcaaggtcgt
     3781 gatgcatggc accgcccacc tccccaacat ggagcgcccc gaagagttca acaggctggt
     3841 gctggagttc ctgcaagacc attagagcgg gttttcggga ggggcgaagc cgtgtcgagc
     3901 aggggtttcg ggtatcagca aaacaaaaga cctgtagccc gatgtccctt cttgcgtgac
     3961 ttcttcatcg ttttggtgta gaaaagacgg gtgtggttgg cggaggttct ccgaagggga
     4021 ggtgaagtca ggatgatatc cagcggggag ttgatcttgc gtctgggggt ggcgggggtc
     4081 ctgggggcgt tggtggggct cgagcgcgag cggttggagt gggcagcggg gatgagaacc
     4141 cacgccctgg tgtcgcttgg ttcagccctg ttcatggtgg tgtctatctt cgggttctcg
     4201 gatatcctga acgagcgcca cgtcgtgttt gacccttccc gcgttgccgc gcaggtagct
     4261 agcggcatcg gcttcatcgg ggctggcacg atcatcttc
//
LOCUS       DBNGVD010000516         6702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4867522, whole genome shotgun sequence.
ACCESSION   DBNGVD010000516 DBNGVD010000000
VERSION     DBNGVD010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4867522"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1503
                     /locus_tag="AC16CM_13950"
     CDS             409..1503
                     /locus_tag="AC16CM_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3140983.1"
                     /translation="MTTSEKIVFTRGVPPPEAFPTEELGACLDAVVREDASIVLQYGQ
                     QRGYAPLREELASEYGVSPAEILVGNGSLQLQDLVSSYLIPPGASVYTEQPSYDRAIK
                     TFRRRGANTIGIPLEEDGISVERLEAALEREVPAFLYLVPDFQNPAGATLSLAKRRRI
                     VGLAGEYGFKVIEDIPYRKLRYEGEDLPLLREIDPTNVITMSSFSKLLSPGMRVGFMI
                     AAEDLIGKVTALGEDTYLSPVLPTQAAVAEYLRRGLLAPNVERLKELYTPRWKAMAGA
                     VRGELPGAQAFIPSGGFFVSVMLPEDANTQDLVGRAAEIGLVLTPGADFFADPDDGGA
                     VEGDRFVRLPFCAVAPDQIEEGVRRLAGLL"
     gene            2409..3776
                     /locus_tag="AC16CM_13955"
     CDS             2409..3776
                     /locus_tag="AC16CM_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3140984.1"
                     /translation="MDDEHVSHGTEHADLKRGIGPWLLFFFVLGDIVGAGIYALVGEV
                     GAVVGGAIWSAFLCAFVLAIFTAASYAELVTKYPRAGGSATYVNNAFRNSFVSFMVAF
                     AVAAASLTSASVLSLAFSGDYLLQFISVPSLLAALVFVVVVGCINFYGISESVRLNLI
                     FTCIEISGLMLIVLIGIVTLGSGTGEPARAFEFKEGASLLPAILAGTVLAFYALIGFD
                     DAVNVAEEVRHPSRIYPYAIFGGLLAAGVIYLLVTFTASMVVPTTQLAESSGPLLEIV
                     ERSPFALPPLLFAGIALIAVSNGALINMIMASRVIYGMADQGVLPAVFSRVHPRRLTP
                     WVAILFTTLVVLTLLATIGRNPDALDTLSTTTVVLLLLAFIMVNIAVLVLRNDEVEHE
                     HFRTPTVFPILGILVSASLLIYTAIDDWTVFGLAGLMLGIGVILYGVNVLVKRSLDSD
                     NPRAT"
     gene            4004..5287
                     /locus_tag="AC16CM_13960"
     CDS             4004..5287
                     /locus_tag="AC16CM_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3140985.1"
                     /translation="MYQGLGEEELAERIARARERLGELGVTFTVAGEEEDLERILPVD
                     WVPRIIAADEWRTLSEGLLQRGKALNAWLRGVYGPGQDLVPEEIVRSSVFHRPHDLPS
                     PSAPIHIYGPDMVHLASGEYVVLEDNVRVPSGAAYSEVIRRAAVETMPEVYGAYRAME
                     IYSYYDRLSQTLELAAPEGVEDPRVVVITRGGRDPAFFEHNRIAQACGLTLMTLADCA
                     VSERMVYERESGRKIDVIYRRFDEDYFDTDLPELERVYLEGNVALVNAPGVGVADDKA
                     VFPYVPAMIETYLGESPILRNAPTYALTEEETRAEVLERLPEMVVKPREGYGGQGVLV
                     GPEAQSEDIDATRRRISKDPTQFVAQETLDFSTHLLRNGKDSPTEVFVDLRAFVLPAI
                     DYLMPGGLTRVAQPGTRVVNSTAGGSSKDTLVLAD"
     gene            5503..6336
                     /gene="aac(3)"
                     /locus_tag="AC16CM_13965"
     CDS             5503..6336
                     /gene="aac(3)"
                     /locus_tag="AC16CM_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /protein_id="HIR3140986.1"
                     /translation="MLVTRSELADDLGRLGLGSGSVVMVHCRLSALGRVVGGAEAVVR
                     AIMDVVGPGGTLVAYTGWQDAPPDDIDALDGAERRAYLEGQPVYDPRVALSRRDHGRV
                     PELLRTWPGALHSWHPEAGVAAVGTLAGEITASHPLDDAYGAGTPYARLVELGGRVVM
                     LGAPLDTVTLVHHAEAIAKVPDKRRISYRYPIDEGGERVWRTFSDIDTGEGALPYERV
                     LGKDDYVSYLAGSALAAGCGTSGEFGNATAYLFEAPQLVEHAVAWIEHNFAPGAAEDP
                     P"
     gene            complement(6446..>6702)
                     /locus_tag="AC16CM_13970"
     CDS             complement(6446..>6702)
                     /locus_tag="AC16CM_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3140987.1"
                     /translation="PSYTVGNVLSVQLFEAAVGERPEIFAQMERGEFDALLGWLRENV
                     HRHGKKYEPDDLISNATGRPPETAPYLAYLQRKFGELYGL"
BASE COUNT         1159 a         2089 c         2195 g         1259 t
ORIGIN      
        1 tgaagcggcc gaggccatcc ggtggtacgg gacgtacgtg catctttgtg gtcctcctga
       61 gacggtaggg tcgtgcggcg actggtggtc ctgaggtggg atccaacaac catgatgtac
      121 agacgcaccg cccgaagggg gaagggccag tataagcgat gggcgaaggc cgggaaaggc
      181 tcctgtaagg cccacaggcg cgggggaatc cagccgttat ctttgcggcg agttgtctgc
      241 cgagcttcat cgagtctcgg ctcgctggtc ctggtcccgt agagttgcgc aagtggggcc
      301 ggtgagtcac cggaattcga ggaaatcagt gcggactgtg gttgcgtccg gggcgggcca
      361 gcggatagga ttggcagttc ccggacatag agagcgaggg tcattaatat gacgacgagc
      421 gagaagatag tcttcacccg tggggtccca ccacccgaag cctttccgac tgaagagctg
      481 ggggcgtgcc tggacgcggt ggtgcgcgaa gacgcgagca tcgtcctgca gtacggccag
      541 cagcgaggct acgcgccact acgtgaggaa ctggccagcg agtacggggt gtcgccggca
      601 gagatactcg tcggcaacgg ctccttgcag ctgcaggacc tcgtgtcgtc ctatctgatt
      661 cctcctggcg cgtccgtata caccgaacag cccagctacg accgcgcgat caagaccttc
      721 aggcgtcgtg gtgcgaacac gatcggcatc ccgctcgagg aggacggcat tagcgtcgaa
      781 cgcctggaag cggcgctcga gcgtgaggtg cccgcgttcc tgtacctggt gcccgacttc
      841 cagaacccgg cgggggccac gctgtccctg gcgaagcgaa ggaggatcgt agggctcgcc
      901 ggggagtacg gcttcaaggt catcgaggac attccctacc gcaagctgcg ttacgagggg
      961 gaggaccttc cgctcctgcg tgagatcgac cccacaaacg tcatcacgat gagctcgttc
     1021 agcaagctcc tgagccctgg tatgagggtg gggttcatga tcgccgcgga agacctgatc
     1081 gggaaggtta cggccctagg cgaggataca tatctctcac cggtgctccc cacgcaggcc
     1141 gcggtcgccg agtacctgcg acgggggctg ctcgccccga acgtggagcg cctcaaggag
     1201 ctctacaccc cgcgctggaa ggctatggcc ggcgccgtac gcggcgagct gccaggcgcc
     1261 caggccttta tcccatcggg gggcttcttc gtgagcgtga tgctcccgga agacgccaac
     1321 acccaggacc tcgtggggcg cgcggccgag atcgggcttg tccttacgcc gggcgcagat
     1381 ttcttcgccg accccgacga cggcggagcg gtagaaggcg acaggttcgt gcggctgccg
     1441 ttctgcgccg tagcccccga ccagatcgag gagggcgtca ggcggctggc cggcctgctc
     1501 tagtagccca ccactggagc tcgcccccgt ggagagccca tagcggtcat ccttcagctg
     1561 aaaccgacga ccgaaggatg accgcgtcgg gcgggaggtc ggattccgac ctcccgccct
     1621 gacctagcgt ccgaacacct ctagttctgc tgcgcgaacc acccggtctt cattcgatcc
     1681 cgatgtacag tcgctgttgt tcgcagcgtc ggggtcgccc tggtatgcgg gacccccggt
     1741 gcactggttc gagatcatcc gcagctggac gtgcgtggcc gtcgagcctt cgacgtcata
     1801 gctctggaac tggagctacg gcactaccgg gtgcggccgg ccagacgagg ccggccccgt
     1861 gctcgacgat gccggactgg tgaagacggt cgtgaagccg cggtctgccg ccgtcttgcc
     1921 ttcttgcaac gtcgtcggcg ctaccgtctg cgagctttcc cccgagtacc acagcccctc
     1981 gtgctgcgcg aacttgcgta cctctacgca agcatattca ttgttcgaaa cccacgacag
     2041 atcgtgtggc ttcatagctt gttcgcaggc tccgcggttc ttgacgttac acgcgaaccc
     2101 ggcggcgtgg tggccgcgac gtgcggtcaa accccgacgc tctcggtgtg ttcgggccgg
     2161 cgaagagaca cgagccgggg acccatgctg ggccccagta caacgaccag atgcagccgt
     2221 atggttaccc ggatattcta ccctgtcgcc ctggtttgtg gagggggtat atcccgccag
     2281 aggacgacgt ggggcgcccc gggaagaacg ttcaatatcg cgtgaatact ttacgttagt
     2341 cctgtaacat gggaactgga gtgtagaaag gaggccctga ggcttcggtc agcgggcggg
     2401 gagggaatat ggacgacgaa cacgtatctc acggtaccga gcacgctgat cttaaacgcg
     2461 gcatcgggcc gtggttgctc tttttcttcg tgctcggcga catcgttggg gccggcatct
     2521 acgcgctcgt cggcgaggtc ggggccgttg tggggggtgc catctggagc gcgttcctgt
     2581 gcgccttcgt gctggcaatc ttcaccgccg cctcctacgc cgagctcgtt accaagtacc
     2641 ccagggccgg tgggtcggca acctacgtca acaacgcctt ccgaaactcg ttcgtcagct
     2701 tcatggttgc cttcgcggtt gcggcggcca gcctcacgtc ggccagcgtc ctgagcctcg
     2761 cgttctccgg cgattacctt ctccagttca taagcgtgcc gagcctgctc gccgcgctcg
     2821 tgttcgtcgt ggtcgtcggc tgcatcaact tttacggtat ctccgagtcg gtcaggctca
     2881 acctgatatt cacctgcatc gagatctcgg gtctgatgct gatcgtcctg atcggcatcg
     2941 tgacgctggg tagtggaacg ggtgagccgg ctagggcgtt cgagttcaaa gaaggcgcga
     3001 gccttctccc ggccatcctg gccggcacgg tccttgcctt ctacgccctc ataggtttcg
     3061 acgacgcggt caacgtggcc gaggaggtga ggcatcccag cagaatctac ccgtatgcga
     3121 tcttcggcgg gcttctcgcc gccggggtca tatacctgct cgtaacgttc acggcctcga
     3181 tggtggtgcc gaccacccag ctcgcagagt cgagcggacc gttgctcgag atcgtggagc
     3241 gtagcccgtt cgcgcttccg ccgctcctgt tcgcgggcat cgcgttgatc gctgtctcga
     3301 acggggccct tatcaacatg atcatggcct cccgcgtgat ctacgggatg gcggatcagg
     3361 gcgtcttgcc ggcggtgttc tccagggtcc accccaggcg ccttacccca tgggtcgcaa
     3421 tccttttcac cacgctcgtg gtgctgacgc tcctcgccac catcggccgt aaccccgacg
     3481 cactcgatac cctctcgacc accaccgtgg tgctgctgtt gctcgcattc ataatggtga
     3541 acatcgcggt cctcgtgctt cgtaacgatg aggtggagca cgagcatttc cgcacgccca
     3601 cggtgttccc catcctgggc atcctggtct ccgccagcct cctgatctac acggccatag
     3661 acgactggac ggtgttcggg ttggctggcc tgatgcttgg gatcggcgtc atactctacg
     3721 gcgtgaacgt ccttgtgaag aggagcctgg acagcgacaa cccccgggct acgtaatagg
     3781 tgagccccgc gccctgcccg agggccaaaa gttcacttga cctgacgacg catcttgcgt
     3841 cgtcgggcca attacgcagc gctctgtctt ccctattttc tagaggggca cggccccagg
     3901 aacgtatcag cgacgggagg aaagaacact caggccatcg tacttcaacg agatctatga
     3961 cgacgcgggc acgctgcgta gcccctacgc ggcgttgcgt gaggtgtacc agggactggg
     4021 cgaagaggag ctggcagaga ggatcgcgcg cgccagggag aggctcgggg agcttggtgt
     4081 gacctttacc gtcgccggtg aggaggaaga cctcgaacgc atcttgcccg tggactgggt
     4141 acctcgcatc atagccgccg acgagtggcg aaccctgtcc gaggggctcc tgcagcgggg
     4201 aaaagcgctc aacgcgtggt tgcgcggcgt ctacggtcct ggccaggatc tcgttcccga
     4261 ggagatcgtg cgcagcagcg tcttccacag gccgcacgac ctccccagcc cctcggcgcc
     4321 gatccacatc tacggacccg acatggtgca cctggcttcc ggagagtacg tggtgctgga
     4381 ggacaacgtg cgcgttccaa gcggggccgc ctactccgag gtcatcaggc gggccgcggt
     4441 ggagactatg ccagaggtct acggggcgta cagggcgatg gagatctact cctactacga
     4501 ccggctgagt cagacgctcg agctcgccgc tcccgagggc gtggaagatc cccgcgtggt
     4561 ggtcatcacg cgcggaggac gagatcctgc gttcttcgag cacaacagga tagcccaggc
     4621 ctgcggtctt acactgatga cgctcgccga ctgcgcggta tccgagcgga tggtctacga
     4681 gcgagagagc ggccgaaaga tagacgtgat ctaccgccgt ttcgacgagg attacttcga
     4741 tacggacctg cccgagttgg aacgcgtcta cctggaaggt aacgtcgcgc tcgtcaacgc
     4801 ccccggcgtc ggggtcgctg acgacaaggc cgtgtttccc tatgtgccag ccatgatcga
     4861 aacttacctt ggagagagcc ccatccttcg gaacgcgccg acctacgcgc taacggagga
     4921 ggaaacccgg gccgaagtcc tcgaacgcct ccccgagatg gtcgtgaaac cccgcgaggg
     4981 ctacggagga cagggagtgc tggtcggtcc tgaggcccaa tccgaggata tcgacgccac
     5041 ccgccgccgc ataagcaagg atcccacaca attcgtggcc caggagacgc tcgatttctc
     5101 gacccatctt ttgaggaacg gcaaagactc gcccaccgaa gtattcgtgg acttgcgggc
     5161 cttcgtactt cccgctatcg actacctgat gcccgggggc ctgacccgcg tcgcccaacc
     5221 aggcacccgc gtcgtgaact ccaccgccgg cggcagcagc aaggataccc tggtactggc
     5281 ggactagcct ggcacgctca ggcctgcgtc ctcccctcgg caacatggcg cttcgcgcct
     5341 tgtcggcaca cctctagact tcggctctcg cttctcgtcc gctcgaaaac ggggagccac
     5401 ggagtcgagg agggagcggg tcctcgagat gggccgccgt ctgaccggat tacttatcat
     5461 ctggtgtggt cggggtggtg gcagcctgtt aagttgtggg tcatgctggt aacgcgttct
     5521 gagctggccg acgatctcgg acggttgggg ttgggatcgg gctcggtggt catggtccat
     5581 tgccgcttga gtgcgctggg gcgtgtggtg gggggagccg aggctgtagt acgggccatc
     5641 atggacgttg tgggtcccgg gggaacgctc gttgcctaca cgggatggca agacgcgccg
     5701 cccgacgaca tcgacgccct ggacggtgct gagaggcgag cctatctgga ggggcagccg
     5761 gtctacgacc ctcgggtcgc gctatcgcgc cgggaccatg gccgggtccc ggaactcttg
     5821 aggacctggc cgggtgcgct tcacagctgg catcccgagg cgggggtcgc ggccgttggg
     5881 acgctcgccg gggagatcac cgcctcccac cccctcgatg atgcctacgg cgccgggacg
     5941 ccctacgcca ggctggtaga gcttggtggc cgggtcgtga tgcttggtgc gccgcttgat
     6001 accgttaccc tggtgcatca tgcggaggcg atcgcaaagg tcccggacaa gcgccgtatc
     6061 tcctaccgat acccgatcga cgaaggtggg gagcgcgtct ggaggacgtt ctccgatatc
     6121 gacacggggg agggggcact tccttacgag cgtgtcctgg gcaaagacga ttacgtctcc
     6181 tacctcgcgg gctcggcgct cgcagcgggt tgtgggacga gcggggagtt cgggaatgcc
     6241 acagcttacc tgttcgaggc cccacaactg gtcgagcacg cggtagcctg gatcgaacac
     6301 aacttcgcgc ctggagccgc cgaggaccca ccctagcctc gtcctctgca tgccaatggg
     6361 gaccctctcg cagcgccgca tcctatacgg tagccgcagg ttcccgcgct gaagccgaac
     6421 gagcctggct acagcactga tgcctctaga ggccgtacag ctcgccgaac tttcgttgca
     6481 agtacgcgag gtaaggtgcc gtctccggag ggcggccggt ggcgttcgag atcaggtcgt
     6541 ccggctcgta cttcttgccg tggcggtgta cgttctcccg cagccacccg agaagggcgt
     6601 cgaactcgcc gcgctccatc tgggcgaaga tctcgggccg ctcgcctaca gcggcttcga
     6661 agagctggac cgagagtacg ttgccaacgg tgtagctggg aa
//
LOCUS       DBNGVD010000517         9056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_132107, whole genome shotgun sequence.
ACCESSION   DBNGVD010000517 DBNGVD010000000
VERSION     DBNGVD010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_132107"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..623)
                     /locus_tag="AC16CM_13975"
     CDS             complement(171..623)
                     /locus_tag="AC16CM_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3140988.1"
                     /translation="MEARDRNYGVVGDEVAGEEGTRSQGSVTMQTQEEKTWSVLAHLS
                     IFLNLVTGFLGPVAALVIWLVYRDRSSRVAFQALQSMWYQVAWLVILAVGWTVTTLLM
                     IILIGFLLVPVMALITVVPFVHSAYAAYRVSRDGEYRYPLIADLIEGR"
     gene            811..1290
                     /gene="lspA"
                     /locus_tag="AC16CM_13980"
     CDS             811..1290
                     /gene="lspA"
                     /locus_tag="AC16CM_13980"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3140989.1"
                     /translation="MTRRASLLWAVAIAVAVFSLDQGIKSLVEGSMRIGQSISLVPGL
                     LDITYIKNDGGAFGILGGSRLILLAGSAVAVVVVLWMLLSGRPTRLTTVGCGLILGGA
                     AGNLLDRLASGAVTDYVHFSFWYIFNAADTAIVVGVGLLLLSALRPEPVGQRQRTGG"
     gene            1826..2023
                     /locus_tag="AC16CM_13985"
     CDS             1826..2023
                     /locus_tag="AC16CM_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3140990.1"
                     /translation="MTERERRAGSGGTEDNVRDELDEAAERGKHGRDASKGAVEEEEQ
                     TGGPGGVKDNVEDEWDESQER"
     gene            complement(2083..2514)
                     /locus_tag="AC16CM_13990"
     CDS             complement(2083..2514)
                     /locus_tag="AC16CM_13990"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3140991.1"
                     /translation="MPRRYIVLLILFCIALNAAGFLWKLFEPIPLYDEIAHFLTPFTL
                     VMILAEIIYRSGGHDDFFNTPRRAVITGSVIGMLGAVGWEGIEILLSLMGVAISNTLP
                     DSVFDVVLGVLGGAAGAWAADRYLDRIFKRSRTNPHQRRVR"
     gene            complement(2518..3150)
                     /locus_tag="AC16CM_13995"
     CDS             complement(2518..3150)
                     /locus_tag="AC16CM_13995"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3140992.1"
                     /translation="MTSTFETPLEAATRTTGSLIVITGSMFSGKTEELIRRVRRALYA
                     RRSVQVFKHAIDTRTERTEIRSHNGVPHEATAVATSGELLERVDETTDVVAIEEAQFF
                     DGDIVESCRDLADGGYVVLATGLDMDFRGEPFGPMPRLLAEADEVVKLRAICARCGRD
                     ASRSQRLINGRPAPVSAPTILVGAEESYEARCRHCHEVPEGVFQYGLPGL"
     gene            complement(3235..4128)
                     /locus_tag="AC16CM_14000"
     CDS             complement(3235..4128)
                     /locus_tag="AC16CM_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3140993.1"
                     /translation="MRAVRSLLAVPATRRNMVEKALASNADAVFLDLEDAVAPEDKAS
                     ARGDVIRALQELDWRDRPTLFRANALGTPFFYRDLIEVVEAAGGCLDAVMIPKVNRPQ
                     DLHAVSVLLEGLELATGLDVGKTRLQAQIESAEGLVNADTIARATDRLEAIHFGPGDF
                     AATLRMPLTSIGVMDEWDEAYPGHRFHYVMQRIVVAARAAGVLVLDGPVADYGDEEGL
                     RESCLRARSLGFDGKWCIHPAQISVVNGAFSPTEREVEWARKVVAAYAEANAAGSGSV
                     SVDGQMVDAASIRMAQNTLEN"
     gene            complement(4130..5545)
                     /locus_tag="AC16CM_14005"
     CDS             complement(4130..5545)
                     /locus_tag="AC16CM_14005"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3140994.1"
                     /translation="MAGAIGAGSPEDIGQSTRAAGDSIQQTIGEARAESGSYVFPAFD
                     DHHPPEKDLIDDCVHCGFCLPTCPTYVLFGEEMDSPRGRIYLMNKGLKEEPMNDEMVR
                     HWDLCLGCMACVTACPSGVQYDKLIEATRQQVERRYERRADDRAFREMIFQFFPYPNR
                     LRALAAPMRLYQRFGIGDKLRKAGIMQRIPARMRAMEALLPDLPKEEKIPEVTGPVGE
                     RRRRVGVLTGCVQRVFFSPVNAATVRVLAAEGCEIIAPEQGCCGALSTHAGREEESLD
                     FARKTIDTFEHLNLDNVIVNAAGCGSTMKEYGYLLRDDPDYAERAEAFSATVRDVSEF
                     LQELGPVAERNPLPVTAAYHDACHLAHAQGVRKQPRQTLKQIPGMDVKEIAEAEVCCG
                     SAGIYNMVEPEPAAELGERKANNILKTGAQMIVTSNPGCMLQIQASLKRMGHDGIPMV
                     HPMEVLDASLRGEPVETLLDR"
     gene            complement(5641..6921)
                     /locus_tag="AC16CM_14010"
     CDS             complement(5641..6921)
                     /locus_tag="AC16CM_14010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3140995.1"
                     /translation="MQTQNLPTEELQNIVGADNVREATAEDAVEGVEPSLVVEPGTIE
                     ETSEVMKLAGREGLAVSPRGGGTKMGLGNPPTRVDLILSTSRMNEIIEHVPGDQIVRT
                     QAGLKMEDLQESLAESDQLLGIDPQEEGGTVGGLVAANASGPRRLRYGTVRDLIIGIK
                     VVLADGTVAKAGGKVVKNVAGYDLSKLFTGSLGTLGVIAEANFRLHPIRETARTVFVE
                     VDEPDQIPEVAQALTHSSASQFVLDALEMRWEDDRGVIAALFEGIEPAVEAQSSAAVE
                     LLRSHGEAKLLGKEDGDEFWDDFTRRPWAKGDVALKIGAPPADLSAVLDSVRGAAERA
                     GVEARLSGHAGIGVTFAGLSGEDDGLVAVVEEVREIRVRRGGSVVVQEAPLSVKERLE
                     VWGPVGDYLGLTRRVKEKFDPGYNMNPGRFLGGI"
     gene            complement(6921..8375)
                     /locus_tag="AC16CM_14015"
     CDS             complement(6921..8375)
                     /locus_tag="AC16CM_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3140996.1"
                     /translation="MDRGQLIETMQEILGPEGVIHKREELRTYECDGLMNYRVIPDLV
                     VLPESAEQVQQVVRACYDEGVPFVARGSGTGLSGGALPVESGVLIVLTRLRRILEVDI
                     PNGRVVVEPGVINVWVSQEVAGEGYYYAPDPASQVVSSIGGNLAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVVHIGGGKSPDAPGYELLGAFVGSEGTLGIATKITLRILRQPE
                     AVRTLLAAFEDTEEAGGAVSGIIGGGILPAAIEMMDSLTIQAVEKQVSPGFPEAGAIL
                     IVELDGPEAEVAKQFDQVIQVCENNNASEIRVAEDDEERALFWKGRKAAFGAMGSISN
                     DYYVQDGVVPRTELGATLKKIAELSEEYGLRVANVFHAGDGNLHPLVLYDNDVPGEAD
                     KAEELAGEIVFACLEHGGSLTGEHGIGRDKMKYMPQMFTSDDMDVMQLLRCAFDPHRI
                     CNPGKIFPTPRLCGERPGPFHMHPVQKAGLAENF"
     gene            complement(8397..>9056)
                     /locus_tag="AC16CM_14020"
     CDS             complement(8397..>9056)
                     /locus_tag="AC16CM_14020"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3140997.1"
                     /translation="GEDGVLEGVREGALIVDMSTISPVVTEELAGRAEEHGASMLDAP
                     VSGGDVGAIDGTLSIMVGGSEEDFGRALPLFEVMGKTVTHVGPVGTGQVVKAANQIVV
                     ALTIEAVSEALVLGSKAGVSPEKILDVLGGGLAGNKVMEVKREKMLGHSFDPGFRVEL
                     HHKDLGIALAAGREYGVSLPVTAVVDQMFETLKMLGRGGQDHSALLTLVEESSGHEIG
                     S"
BASE COUNT         1485 a         3046 c         2897 g         1628 t
ORIGIN      
        1 gtggtcccgt tcctccatag cgcgtacgcg gcctaccgcg tgagcaggga cggcgagtac
       61 cgttatcccc tggtagccga cctgatcgaa ggccgctagg agaccgttcg agaacaccgg
      121 cgcccccgaa ctcctgaggt tcgagggcgc cggtgttctc gaacggcctc ctagcggcct
      181 tcgatcaggt cggcgatcag cgggtaacgg tactcaccgt ccctgctcac gcggtaggcc
      241 gcgtacgcgc tgtggacgaa cgggaccacc gtgatcaggg ccattaccgg aacgagaagg
      301 aagccgatca ggatgatcat caggagcgtt gtcacggtcc atccaacggc caggatgacc
      361 agccaggcca cctgatacca catggactgc aacgcctgga acgccacccg gctcgacctg
      421 tccctgtaga ccaaccagat aaccagggcc gccactgggc cgagaaaccc tgtaacgagg
      481 ttgagaaaga tgctcaggtg cgccagcacc gaccacgtct tctcctcctg ggtctgcatg
      541 gtcaccgagc cctgcgaccg ggtgccctcc tcccccgcta cctcgtcccc gaccacccca
      601 taattgcgat ctcttgcctc caccagcact cctctctgtc gatctacgat ctacgcaaac
      661 ataaaatacc ccctaaccct ctacccgcca tcggtccctg gaccgaataa agctagtccc
      721 gtagacgcag cccgcttcgc ctacgcgtgc tgcggggcgt gctaggattt gcgggcttat
      781 gggtgatcct gtcgtgtatt gcgtctcctt gtgacccgtc gggccagctt gctctgggcc
      841 gtggctatcg cggttgcggt gttctctctc gaccagggga taaagtccct ggtagagggt
      901 tccatgcgga tcgggcagag cattagcctc gtgcccggcc tgctcgacat cacctacatc
      961 aagaacgatg gcggagcctt cggtatcctc ggggggagcc ggctgatcct gctcgccggg
     1021 agcgccgtcg cggtcgtggt cgtgctctgg atgctcctct cggggaggcc gacgaggctc
     1081 accacggtag ggtgcggcct gatcctgggc ggggcggcgg gcaatctgct cgaccgcctg
     1141 gccagcgggg cggttacgga ctacgtacat ttcagcttct ggtacatctt caacgccgcc
     1201 gacacggcca tcgtggtcgg cgtcggcctg ctactcctgt ccgcccttcg cccggaaccg
     1261 gtcggccaga gacagcgcac gggaggctga ccccgctgcc cccgtccggc aactcggccg
     1321 aaatatacgt cttctgggaa atgtgggggc cgcgaaggcg ctgccacgct tcactagtga
     1381 ggcaagccag ccatgggacc ctactctgaa gcgttgccaa aggtccgcga gggtatgacc
     1441 ccggaagaga atgctgccac gcatccccct ggctacctgc tcgacgagtc gaaccccgat
     1501 atcgtcacgc tacgacgcaa ggacggcacc ttcgtcgccg ccttcagcac ccagggcgcg
     1561 acgaaggagg gcatcagaga caccgccatg gaagactccg ggaaccctac agcccccccg
     1621 caaggcacaa ggaagaggac cggtaggaga cccggcccgc gtctcaacgc aagagtgaag
     1681 atgccgtcct cttgtagttg gtggggaacg tcgcaaacga tcctgcctat tcctcccgac
     1741 acagccgggg tgggatctca ccggcccggc caccttgctg cgtggtagca tggaccttac
     1801 gcgagaccga ccggaaggag aacgcatgac cgagcgtgaa cgccgcgcgg gctcaggcgg
     1861 taccgaggac aacgtacggg acgagctcga cgaggccgca gagcggggca agcacggccg
     1921 agacgcctcg aagggggccg tcgaggaaga ggagcaaacg ggcggcccgg gcggcgtcaa
     1981 ggacaacgtc gaagacgagt gggacgaatc tcaggaacgt tgaaccgagc ctctgcatag
     2041 ggacggttcg cgatccgccc gggaactgcg ccggcctccg tcctaacgaa cccggcgttg
     2101 atgggggttc gtgcgggagc gcttgaagat gcgatctagg taacggtcgg cggcccaggc
     2161 accggcggcg ccccccagga caccgagtac gacgtcgaac acggaatccg gcagggtgtt
     2221 ggagatggcc acgcccatca gggagagcag gatctctatg ccctcccaac ccacggcgcc
     2281 gagcatccct ataaccgagc ctgtgataac ggccctcctg ggcgtgttga agaaatcgtc
     2341 gtgtcccccg ctccggtaga tgatctcggc caggatcatg accagcgtga agggcgtgag
     2401 gaagtgggct atctcgtcgt agagcgggat gggctcgaag agcttccaga ggaacccggc
     2461 cgcgttcagg gctatgcaga acaggatgag gagtactata tatctcctgg gcatcttcta
     2521 gagtcccggg agcccgtact ggaacacgcc ctccggaacc tcgtggcagt gtcggcacct
     2581 cgcctcgtag ctctcctctg ccccgacgag tatggttggc gcggagacgg gggccggcct
     2641 cccgtttatg agccgctgcg agcgtgaggc gtcccttccg caccgggcgc agatggccct
     2701 gagcttcacc acctcgtcgg cctcggcgag aagcctcggc attggcccga agggctcgcc
     2761 gcgaaagtcc atgtcgaggc ccgtcgcgag taccacgtag ccgccgtcgg cgagatcccg
     2821 gcacgactcg actatgtccc cgtcgaagaa ctgtgcctct tcgatggcta ccacatccgt
     2881 ggtctcgtcc accctctcca gaagctcccc gctggtcgcg acggccgtgg cctcgtgtgg
     2941 gacaccgtta tgggagcgta tctctgtacg ctccgtgcgg gtgtctatgg cgtgcttgaa
     3001 gacctgcacg ctgcgcctgg cgtagagggc gcggcggaca cgccggatca actcctccgt
     3061 cttgcccgag aacatcgagc ccgttatgac gatgagggag ccggtcgttc tcgtggcggc
     3121 ttccaggggc gtttcgaagg tgctggtcat ggcccgtatt ctacgcccca cgcgcgcctt
     3181 tacatggaac agtgagccct ccatcgttcc gaccgaccag cggctctctg attcctaatt
     3241 ctccagcgtg ttctgggcca tccttatgga ggcggcgtcc accatctggc cgtccacgct
     3301 gaccgagcca gagccggcgg cgtttgcctc cgcgtaggcg gcgaccactt tccgtgccca
     3361 ctccacctct ctctcggtcg gcgagaaggc cccgtttacg acggagatct gggcgggatg
     3421 gatgcaccac ttgccgtcga agccgaggga gcgggcgcgg agacagctct cacgaaggcc
     3481 ctcttcgtcg ccgtagtcgg cgaccggtcc atcgaggacg agcacgcccg cggcgcgggc
     3541 ggctaccacg atacgctgca tgacgtagtg gaagcggtgg ccggggtagg cttcgtccca
     3601 ctcgtccatc acgccgatgc tcgtcagggg catccgcagg gtcgccgcaa agtctccggg
     3661 accgaagtgg atcgcttcga ggcgatccgt ggcgcgggct atagtgtcgg cattgaccag
     3721 gccctcggcg ctctctatct gggcctggag ccttgttttg cccacgtcca ggcccgtcgc
     3781 gagctccagc ccctccagca ggacagagac ggcgtggagg tcctggggcc ggttcacctt
     3841 ggggatcatg acggcatcga ggcagccgcc ggccgcttct accacctcga tcaggtcgcg
     3901 gtagaagaac ggcgtaccga gggcgttggc cctgaagagc gtgggacggt cgcgccagtc
     3961 gagttcctgc agcgcgcgga tcacatcccc gcgcgctgag gccttgtctt cgggggccac
     4021 cgcgtcttcg aggtccagaa agactgcgtc tgcgttcgag gcgagtgctt tctccaccat
     4081 gttccgcctg gtggcgggaa cggcgaggag ggagcggacg gccctcacgc tatctgtcta
     4141 gcagcgtctc taccggttct ccgcgcaggg aggcgtcgag cacctccatg gggtggacca
     4201 tcggtatgcc gtcgtggccc atcctcttga gggaggcctg tatctggagc atgcagccgg
     4261 ggttggaggt cacgatcatc tgcgccccgg tcttcaggat gttgtttgcc ttgcgctccc
     4321 cgagttcggc ggcgggctcg ggctcgacca tgttgtagat gccggcggac ccgcagcaga
     4381 cctcggcctc cgctatctcc ttcacgtcca tccccggtat ctgcttgagg gtctgcctgg
     4441 gttgcttcct gacgccctgc gcgtgcgcca ggtggcaggc gtcgtggtag gcggctgtga
     4501 cgggaagcgg gttgcgctcg gccacgggcc ccagctcctg caagaactcc gagacgtcgc
     4561 ggacggtggc gctgaaagcc tcggccctct cggcgtagtc ggggtcgtcg cgcagcaggt
     4621 acccgtactc cttcatcgtg gagccgcagc ctgcggcgtt cacgatcacg ttatccaggt
     4681 tcagatgctc gaaggtgtct atcgtcttgc gggcgaagtc gagtgactcc tcttcgcggc
     4741 cggcgtgggt ggagagggca ccgcagcagc cctgttcggg cgctatgatc tcgcagccct
     4801 cggctgcgag gacccggacg gtggcggcgt tcaccggcga gaagaagaca cgctgcacgc
     4861 agccggtcag gacgccgacc ctgcggcgtc tctcgccgac cgggcccgtt acctctggga
     4921 tcttctcctc cttcggaagg tctggcagga gcgcctccat ggcgcgcatc ctggccggga
     4981 tgcgctgcat gatgccggcc ttgcgcagct tgtccccgat gccgaagcgc tggtagaggc
     5041 gcatgggcgc ggcgagggcg cgcagccggt tgggatatgg gaagaactgg aagatcatct
     5101 ccctgaaggc cctgtcgtcg gcgcggcgct cgtagcggcg ctcgacctgc tggcgcgtgg
     5161 cctcgatgag cttgtcgtac tgcacgccag aggggcaggc cgtgacgcag gccatgcacc
     5221 cgaggcaaag gtcccagtga cgaaccatct cgtcgttcat gggctcctct ttgaggccct
     5281 tgttcatcag gtagatgcgc ccgcgcggcg agtccatctc ctccccgaag agaacgtagg
     5341 tcgggcacgt cggcaggcag aagccgcagt ggacgcagtc gtctatgagg tccttctcgg
     5401 gcgggtgatg gtcgtcgaag gcggggaaca cgtaggagcc gctctcggcc cttgcctcgc
     5461 cgatcgtctg ctggatagag tcgcccgcgg cgcgggttga ctggccgata tcctcggggc
     5521 ttccggcgcc gatggcaccg gccatcgcct cgccaccagt cgtggcctcg gggttctgac
     5581 gctcggtcgc gtccgtggcg ctgtagcggt cctccccgcc ggaaccgttg cgttcggtca
     5641 ctagatgcct cccaggaatc taccggggtt catgttgtag cccggatcga acttctcttt
     5701 gacccgccgt gtgaggccga ggtagtcgcc aacaggaccc cacacctcca gccgctcctt
     5761 gacggagagg ggggcctcct gcaccaccac gctgccgccc ctgcggaccc gaatctcccg
     5821 cacctcctcc acgacggcga cgagcccgtc gtcttcgccg gagagccccg cgaacgttac
     5881 accgatcccg gcgtggcccg agagacgggc ctcgacgccg gcgcgctccg ccgccccgcg
     5941 gaccgaatcg agaacggccg agaggtcggc gggcggggcg cctatcttga gcgccacgtc
     6001 ccccttggcc cagggccgtc gcgtgaagtc gtcccagaac tcgtcgccgt cttccttgcc
     6061 gagcaacttc gcctcgccgt gcgaacgcag tagctccacc gcggcggatg actgcgcctc
     6121 gacggccggc tctatgccct cgaacagcgc ggcgatcacg ccgcggtcgt cctcccagcg
     6181 catctccagg gcgtcgagca cgaactggct ggcgctcgaa tgcgtgagag cctgcgccac
     6241 ctccgggatc tggtcaggtt cgtcaacctc cacgaaaacg gtcctggcgg tctcccggat
     6301 cgggtgtagc ctgaagttgg cctcggcgat gacgccaagg gtcccgaggg agccggtaaa
     6361 cagcttggag aggtcgtaac cggccacgtt cttgacgacc ttgccgccgg cctttgccac
     6421 cgtgccgtct gcgagcacca ccttgatgcc gatgatcaga tcccgcaccg tgccgtacct
     6481 gaggcgacgc ggtcctgagg cattggcagc caccaggccg ccgacggtcc ccccttcttc
     6541 ctgggggtcg atgccgagca gctggtccga ttcggcgagg ctctcctgaa ggtcctccat
     6601 cttcaagccg gcctgggtgc gcactatctg atcccccggc acgtgctcga tgatctcgtt
     6661 catgcgcgag gtgctgagga tgagatccac ccgcgtcggc gggttcccga ggcccatctt
     6721 cgtgccgccg ccgcgcggcg agacggcgag cccctcacga ccagcgagct tcatgacctc
     6781 gctggtctcc tcgatcgtgc ctggctcgac cacgagggag ggttcgacgc cttctacggc
     6841 gtcctcggcc gtggcctccc tcacgttgtc cgcgccgacg atgttctgca gttcttccgt
     6901 tggcaggttc tgggtctgca ttagaagttc tccgcgagac cggccttctg gaccgggtgc
     6961 atgtggaagg gaccgggacg ctcgccacag aggcggggcg tcggaaagat tttgccgggg
     7021 ttgcagatcc tgtgcgggtc gaaggcgcac ctgagcagct gcatcacgtc catgtcatcc
     7081 gaggtgaaca tctggggcat gtacttcatc ttgtcgcgcc cgatgccgtg ctcccctgtg
     7141 agcgacccgc cgtgctccag gcaggcgaac acgatctccc cggcaagctc ctcggccttg
     7201 tcggcctcgc cgggaacgtc gttgtcgtaa aggacgagcg ggtggaggtt gccgtctccg
     7261 gcgtggaaca cgttggcgac cctcaggccg tactcctcgc taagctccgc tatcttcttg
     7321 agggtggccc cgagctccgt gcgcgggacg accccgtcct ggacgtagta gtcgttggag
     7381 atggagccca tggcgccgaa tgcggccttg cggcccttcc agaacagggc gcgctcctcg
     7441 tcgtcctcgg cgacccttat ctccgaggcg ttgttgttct cgcagacctg tatcacctgg
     7501 tcgaactgct tggcgacctc ggcttcgggg ccgtcgagct ccacgatcag gatcgcgccc
     7561 gcctcgggga agccggggga gacctgcttc tcgaccgcct ggatggtaag cgagtccatc
     7621 atctctatgg cggccggcag gatgccgccg ccgataatcc cggagacagc cccgccggcc
     7681 tcctccgtgt cctcgaaggc cgcgagcaac gtccttacgg cctccggctg gcgcaggatc
     7741 ctgagcgtta tcttggtggc tatgccaagg gtgccctcgg aaccgacgaa ggcccccaga
     7801 agctcgtagc ctggggcgtc gggtgatttc ccgccgccga tgtggaccac cgagccgtcg
     7861 ggcaagacga cctcggcgcc catcacgtgg ttggtggtga agccgtactt gaggcagtgg
     7921 acgccgcccg agttctcggc gaggttgccg ccgatggagg agacgacctg gctcgccggg
     7981 tcgggtgcgt agtagtagcc ctcgccggcg acctcctgcg agacccacac gtttatcacg
     8041 ccgggctcga ccacgacgcg cccgttgggg atgtctacct cgaggatacg ccgcagcctg
     8101 gtcaggacta tcaggacgcc ggactcgacg gggagagccc ccccggagag acccgtgccc
     8161 gagcccctgg cgacgaaggg aacgccctcg tcgtagcagg ccctgaccac ctgctggacc
     8221 tgctcggccg attctggtag gacgacgagg tcggggatca cgcggtagtt catcaggcca
     8281 tcacactcgt aagtacgcag ctcttcacgc ttgtgtatga caccctcggg accgaggatc
     8341 tcttgcatcg tctctataag ttgcccccgg tccatgcgga ccctcctccc ggagccctag
     8401 ctccctatct cgtgccccga cgactcttct acaagcgtca ggagcgcgga atggtcctgg
     8461 ccccctcttc ccagcatctt caaggtctca aacatctgat cgactaccgc ggtgacggga
     8521 agggacaccc cgtactccct gccggccgca agcgctatcc ccagatcctt gtggtgcaat
     8581 tctacccgaa agccgggatc gaaggaatgc ccgagcatct tctcccgttt gacctccatg
     8641 accttgtttc cggcgagccc gccaccgagc acgtccagga tcttctccgg ggagacgccg
     8701 gccttggaac ccaacacaag cgcctccgag acggcctcga tggtgagcgc gaccacgatc
     8761 tggttggcag cttttaccac ctgccccgtc ccaacgggcc cgacgtgggt aacagtcttg
     8821 cccatgacct cgaagagcgg aagcgcccgc ccgaagtcct cctcgctccc gccaaccatg
     8881 atggagagcg tcccgtctat ggcccccacg tcccccccgc ttaccggcgc gtcgagcata
     8941 gaggcgccat gttcttcggc cctaccggcc agctcttccg taacaacggg tgagatcgtg
     9001 ctcatgtcca cgatcagggc gccttccctg acgccctcca gtacgccgtc ttcccc
//
LOCUS       DBNGVD010000518         3410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1955071, whole genome shotgun sequence.
ACCESSION   DBNGVD010000518 DBNGVD010000000
VERSION     DBNGVD010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1955071"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="AC16CM_14025"
     CDS             complement(<1..941)
                     /locus_tag="AC16CM_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3140998.1"
                     /translation="MAANPVSKNTPSSYDASSIQVLEGLEAVKRRPGMYIGSTGPRGL
                     HHLVWEIVDNSIDEALAGYCSEILIVVHPDNSVSVTDDGRGLPVGTMKKYGKSATEVI
                     LTTLHAGGKFDGQGYKVSGGLHGVGASVVNALSAWLEVEIRREGHVWSQRYERGFPQG
                     EITRDRKLKKGEGTGTTITFQPDPEVFTETVDLSFDTLSRRFRESAFLNKGLKIRLLD
                     ERDEDKDVTYQYEGGIRDFVAHINEAKDPVHKTVFYLEREEEVGDVEVALQWNSGFQD
                     SVFTFANNINTHEGGAHLSGFRSALSRTINSYARQKGL"
     gene            complement(1099..2223)
                     /gene="recF"
                     /locus_tag="AC16CM_14030"
     CDS             complement(1099..2223)
                     /gene="recF"
                     /locus_tag="AC16CM_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3140999.1"
                     /translation="MSAYLRAIRLVNFRNYADATALLAPGLNVVVGDNAQGKTNLLEA
                     IAFNVAGSSPRTPKENEVVRWGSDFARTEARIAIGDSPEERSAAIGYAPGQKKRITVD
                     GAPASSLTNYAAGGSGARVVTFFPDDIRVVKGAPSDRREFLDALLSSLRPSYARAVGE
                     YTKAVAQRNQLLRRIRDGYSSERTLGTWDMKVVDLGKTVLAGRGAALPVLEEHFKDSL
                     RALYGPEKAEIRYRHTGTLEEYSEALREAHGADIERGTTSVGPHRDDFEVVLGGVNLT
                     TFGSQGQQRLATLALEFAARDYVRGEVGDDPILLFDDVMSELDERRREYLAEYFLQST
                     QAIISTTNLEYFDEEILRRTRIIRISGGSILETATNGATR"
     gene            complement(2220..2414)
                     /locus_tag="AC16CM_14035"
     CDS             complement(2220..2414)
                     /locus_tag="AC16CM_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR3141000.1"
                     /translation="MRVPTGTTLGQALKVASLVGSGGEAKVLIQAGEVLVNGEVETRR
                     GRRLEAGDVIELDDERLEVG"
     gene            complement(2422..>3410)
                     /gene="dnaN"
                     /locus_tag="AC16CM_14040"
     CDS             complement(2422..>3410)
                     /gene="dnaN"
                     /locus_tag="AC16CM_14040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3141001.1"
                     /translation="SIQTSSPAEVEGEGRVVIPARIFNDIVRSLPGGSFTLEHDASEG
                     TVRLVAGDNEYRIRAYAADDYPQLPHFPEDGTFRMPGESLVETVEKVSRSYSRDETRP
                     VLTGILISFEDSRVKMVTTDSYRLSIKETELATSGFEGSREAIIPARAMQEVSRIFGS
                     EGDKDVEVSLSENQALFRVGDVVFGTRLIDGNFPEYRRLLPDDFEREIAIPREELMGT
                     LRRVNLFAARQTPPVPVSLSFSEGAVEVIVRNGEVGDAHERLSASSEDEFLISFNPGY
                     LLDGVSAIDSDKVVFKLNEPLKPGLIVPEENGDGAEPDFLYLIMPMRDPSRN"
BASE COUNT          556 a         1124 c         1074 g          656 t
ORIGIN      
        1 aggcccttct gcctggcgta ggagttgatg gtgcgggaga gcgccgagcg gaacccggac
       61 aggtgcgccc cgccctcgtg ggtgttgatg ttgttggcga acgtgaacac ggagtcctgg
      121 aagccgctgt tccactgcag cgcgacctcg acgtccccga cctcctcctc gcgctcgagg
      181 tagaagacgg tcttgtggac ggggtccttc gcctcgttga tgtgcgccac gaagtccctg
      241 atgccgccct cgtactggta ggtgacgtcc ttgtcctcgt cacgctcgtc gagcagccgg
      301 atcttcaatc ccttgttgag gaaggccgac tcgcggaagc ggcggctgag ggtgtcaaac
      361 gagagatcga cggtctcggt gaagacctca gggtcgggct ggaaggttat ggtcgtcccc
      421 gtgccctcac ccttcttgag cttgcggtcc ctggtgatct caccctgggg gaagccgcgc
      481 tcgtagcgct ggctccacac gtgcccctcg cgcctgatct cgacctcgag ccacgcggag
      541 agggcgttga ccacggaggc gcctacgccg tgcagcccgc cggagacctt gtagccctgg
      601 ccgtcgaact tgccgccggc gtgtagcgtg gtgaggatca cctccgtggc cgacttgccg
      661 tatttcttca tcgtgcctac gggcaacccc ctgccgtcgt cggtgacgga gacggagttg
      721 tctgggtgga ccacgatgag tatctcggag caataaccgg ccagcgcctc gtctatggag
      781 ttgtctacga tctcccacac gaggtggtgg aggcccctcg ggcccgttga cccgatgtac
      841 attccaggac gccgcttgac ggcctcgagg ccctccagaa cctggatcga acttgcatca
      901 tacgagctcg gggtattctt ggaaacaggg ttagccgcca aagaaccgga ccctcctctt
      961 tgctaggccg gcgcttctgc cggagacgat cttttcatca gagctttgca cctgaagatt
     1021 gtaacacaca agccgggctt tccaaacggt cgaaggacgg cttttatggc ttagataagc
     1081 ctttttcgta ggatgcgact acctcgtggc accatttgtt gcggtctcga ggatggaacc
     1141 accagatatg cgtatgatac gggttctcct gagtatttcc tcatcgaaat actcgaggtt
     1201 tgtggtggag atgatggcct gggtgctctg caagaagtac tcggcgaggt actccctgcg
     1261 gcgctcgtcc agctcgctca tcacgtcgtc gaagaggagg atggggtcat cgcccacctc
     1321 cccccgcacg tagtccctgg ccgcgaactc gagcgccagc gtcgcgagcc tctgctgccc
     1381 ctgcgacccg aacgtggtca ggttcacccc gccgagcacg acctcgaagt cgtccctgtg
     1441 cggccccacc gaggtcgtgc ctcgttcgat gtctgcgccg tgcgcctctc tgagcgcctc
     1501 ggagtactct tccaatgtcc ccgtatgcct gtagcggatc tcggccttct cggggccata
     1561 cagagccctt agagagtctt tgaagtgctc ctcgaggacc ggcagcgccg ccccgcggcc
     1621 agcgagcacg gtttttccga ggtcgacgac cttcatatcc cacgtgccga gcgtccgctc
     1681 ggaggagtag ccgtccctga tgcgcctcag gagctggttg cgctgggcca cggccttggt
     1741 gtactcgccg acggctcggg cgtatgaggg gcgaagcgag gagagtaggg cgtccagaaa
     1801 ctccctgcgg tcggagggag cccccttcac gaccctgatg tcgtcgggaa agaacgtgac
     1861 gacgcgggca cccgagccgc cggccgcgta gttcgtcaga gacgaggccg gcgccccgtc
     1921 cacggtgatg cgcttcttct gcccgggggc gtagccgatg gcggcgctcc gctcctccgg
     1981 ggagtcgccg atcgctattc gggcctctgt gcgggcgaag tccgaccccc agcggaccac
     2041 ctcgttctcc tttggggtgc ggggtgaaga cccggccacg ttgaaggcga tggcctcgag
     2101 caggttcgtc ttgccctggg cgttgtcgcc gaccaccacg ttcaggccag gggccagcag
     2161 cgccgtggcg tccgcgtagt tacggaagtt cacgagtcgt atggcccgca ggtaggcgct
     2221 caaccgacct ccagccgctc atcgtccagc tcgatcacgt cacccgcttc gagcctgcgg
     2281 ccccggcggg tctcaacctc accgttcacc aggacctcgc cggcctggat caggaccttt
     2341 gcctccccgc ccgagcccac gaggctggcc accttgagcg cctggccgag cgtggtgcct
     2401 gtagggaccc gcaacgcgcc ttcagttgcg ggacgggtcg cgcatcggca tgatcaggta
     2461 cagaaagtcg ggctcagcgc cgtcgccgtt ctcctcgggc acgatgagcc cgggcttgag
     2521 cggctcgttt agcttgaaga cgaccttgtc tgagtctatg gccgagacgc cgtctagcag
     2581 gtagccgggg ttgaaggaga tcaggaactc gtcttcgctc gaggcggaca gcctctcgtg
     2641 cgcatccccc acctcgccgt ttcgcacgat gacctcgacg gccccctcgg agaacgagag
     2701 gctgacgggc accggcggcg tctggcgggc ggcgaagagg ttgacccgcc tgagagtgcc
     2761 catcaactcc tccctgggga tggctatctc ccgctcgaag tcgtccggca agagcctcct
     2821 gtactccggg aagttgccgt cgatgagcct ggtgccgaag accacgtcgc ccaccctgaa
     2881 caacgcctgg ttctcggaga gcgagacctc gacgtctttg tccccttcgg agccgaagat
     2941 cctggagacc tcctgcatgg cccgcgccgg aatgatggcc tccctcgacc cctcgaaacc
     3001 gctcgtcgcc agctccgtct ccttgatgct gagccggtac gagtccgtgg tgaccatctt
     3061 tacccgcgag tcctcgaagc ttatgaggat gcccgtcaag acaggcctgg tctcgtcccg
     3121 cgaatacgac cgcgaaacct tctccacggt ctccacgagc gactccccgg gcatcctgaa
     3181 cgtgccgtcc tcgggaaagt ggggaagctg cgggtagtcg tcggccgcgt aagccctgat
     3241 gcggtactcg ttgtccccgg caacgagcct gaccgtaccc tcagaagcgt cgtgctccag
     3301 cgtgaagctc cccccgggca acgacctgac gatgtcgttg aagatgcgcg ccgggatgac
     3361 cacccggccc tcgccctcga cctcagccgg cgaagaggtc tggatggaga
//
LOCUS       DBNGVD010000519         4086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2319572, whole genome shotgun sequence.
ACCESSION   DBNGVD010000519 DBNGVD010000000
VERSION     DBNGVD010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2319572"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC16CM_14045"
     CDS             <1..249
                     /locus_tag="AC16CM_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141002.1"
                     /translation="ARGLPRGLTYLGLATGAAGVLSALPGIGDAGLVFGLVQIVWFIW
                     LGLVMLRSPSENRERTVEMTYQKTDDTIQYRSGNVGAP"
     gene            454..3225
                     /locus_tag="AC16CM_14050"
     CDS             454..3225
                     /locus_tag="AC16CM_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3141003.1"
                     /translation="MVRKHESVDEKPAAPAKSPNPEERLDLLLGHLGTRREGLSEREA
                     SRRLEQHGPNEIQRRKGTSRLAEFGRQFTHPLALLLWAAAVLALVGGTPPLAAAIVAV
                     IVLNALFAFFQEIQAEKATEALRRYLPPRAHVRREGRTVEVEATSLVPGDLVLISEGD
                     RLSADARLIDGSVEVDMSALTGESQPVARGAARTRPAASPLESDDLVFGGTMVTGGEA
                     EGVVYATGMATQLGRIAALTQRVRIEVSPLQAQVNRAARLIAFVAIGAGALFLALGSL
                     VAHLPLADALLFAIGLLVANVPEGLLPTITLALAVGVRRMARRRALLKRLTAVETLGS
                     TDVICTDKTGTLTEGRMVLSRFWADGTELAPGVLGQVNPAVIDREPFSALLRTAVRCS
                     NATLQRTREALKRTGDPSESALLVAAERLGEDVVRAQEERRERRRRLYHFDARVKRMT
                     TVDEEADGSLWYHVKGAPLELIERCTTVRAADGADRKLTEENRQDVRRAFEGYAGEGL
                     RVLGFAERKVSGPEPEDDRDRAESRLTFLGLAALEDPPRPDVAEAVARCRGADIRIIV
                     VTGDHGLTAGAVARKVGITTGKARVVTGPEVDAMSREELDTLLHDTSELVVARSNPET
                     KLHVVDALRAEGHTVAMTGDGVNDAPALRRADIGVAMGDSGTEVAREAATMVLTDDSF
                     ASIVAAVEEGRVVYDNIRKFVTYIFAHATPEVVPFLIYALSGGAVPLPLTVMQILAID
                     LGTETLPALALGREPAEPGVMSRRPRPRGKGIIDGPMLARAWGRLGLVEAALVTGGFF
                     YVLHQAGWSPGEPTGPGSALHGAYLTATAMTFAGITACQVGTAFAARTNHASLREIGV
                     FSNRLLLWGILFELVFGAAIVYLPPLQATFGTDPLGARELALLATFPFVVWGTDELWR
                     WARRRRDAG"
     gene            3570..>4086
                     /locus_tag="AC16CM_14055"
     CDS             3570..>4086
                     /locus_tag="AC16CM_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3141004.1"
                     /translation="MRESEMGLGTRWVRHFSEVGIEDVPLVGGKNASLGEMTRELGTL
                     GIRVPEGFALTADAYRHFLESNELVCPVRDALDGFEPGDVEDLGRKSRLIRDLVLGAE
                     IPSEVKDEVLRGYEELSSRYGEDFTDVAVRSSATAEDLPTASFAGQQESYLNVHGEAQ
                     LLESVRKCFASL"
BASE COUNT          676 a         1305 c         1452 g          653 t
ORIGIN      
        1 gcaagaggac ttcccagggg cctgacctat ctaggcctgg cgacgggcgc ggcgggcgtc
       61 ctctcggcat tgcccgggat cggtgatgca ggactcgttt tcgggctggt tcagatcgtg
      121 tggttcatct ggcttgggct cgtgatgttg cggagcccga gcgagaaccg ggaacgcacc
      181 gtggaaatga cctaccagaa gaccgacgac acgatccagt accggagcgg caacgtggga
      241 gcaccttgag agcgggtgtg cctgtcacca gggacgacgt tctgtttcgc gtgggcgcgg
      301 cggcctgtat ctacccagag ggcgatgggt atgtccgtgg cctagcaggc agagattttc
      361 gcgggttcta tcggaaagac catcgtgatg gctgtgtttc ccggtatctc cgaccagacg
      421 tccgcaggta gaagcaccgg gggctgacaa cgcgtggtcc ggaagcacga aagcgttgac
      481 gaaaagcccg cagccccggc gaagagccct aatccagagg agcggctcga cctcctgctc
      541 ggccacctgg gaaccaggcg ggaagggctc tccgagcggg aggcgtcccg ccgcctcgag
      601 caacacggac ccaacgagat ccagcgccgc aagggcacga gccgcctcgc ggagttcggc
      661 cgccagttca cccacccgct ggcgctgctg ctctgggcgg cagcggtgct ggccctggtg
      721 ggtggcacgc ctccgctagc cgcggcgatc gtggcggtta tcgtcttgaa cgcgctgttc
      781 gcctttttcc aggagatcca ggccgagaaa gctaccgagg ccctcagacg ctacctgccg
      841 ccccgcgccc acgtccggcg cgaaggccgt acggtggagg tcgaggctac cagcctcgtg
      901 cccggtgacc tggttctgat ctccgagggg gaccggctct cggcggacgc gcgcctgatc
      961 gacggttccg ttgaggtgga catgtccgcg ctcacgggcg aatcacagcc cgtggcgcgg
     1021 ggggcggctc gcacaaggcc ggccgcctcg cccctggagt ccgacgacct cgtcttcggg
     1081 ggcacgatgg tgaccggagg agaggccgag ggcgtcgtct acgcgactgg catggccacc
     1141 caactcgggc gcattgccgc cctgacccaa cgcgtaagaa tcgaggttag ccccctgcag
     1201 gcgcaggtga accgggccgc ccgcctcata gcgttcgtgg ccatcggcgc aggagcgctg
     1261 tttctggccc tggggagcct ggtggcacac ctacccctcg ccgacgccct gctcttcgcc
     1321 atagggctgc tcgtcgccaa cgttcccgag ggtctcttgc caaccatcac gctcgcgctc
     1381 gcggtcggcg tgcggcgcat ggctcggcga agggcgctcc tgaagcgcct gaccgccgtg
     1441 gagaccctgg gctccaccga cgttatctgc acggacaaga cgggaaccct caccgaaggc
     1501 cggatggtcc tctcgcgatt ctgggcggac ggcacggaat tggcccccgg agtcctcggg
     1561 caggtgaacc cggcggtcat cgaccgggag cctttctcgg ctcttctgcg taccgcggtg
     1621 cgctgctcca acgccaccct tcagcggact cgcgaggccc tgaagagaac gggcgatccc
     1681 agcgagagcg cgctgctcgt cgccgccgaa cgtctcggcg aggacgtggt gcgtgcgcag
     1741 gaagagcgcc gggagcgtcg caggcggctc taccacttcg acgccagggt caagcggatg
     1801 acgacggtcg acgaggaagc ggacggcagc ctctggtacc acgtcaaggg tgcaccgctg
     1861 gagctcatcg agcgctgcac taccgtgcgc gctgcggacg gcgccgacag gaagcttacg
     1921 gaagaaaatc gccaggacgt gcgccgggcc ttcgagggtt acgctggcga gggcctccgg
     1981 gtcctgggct tcgccgaacg gaaggtatca ggtcccgagc ccgaggacga tcgtgaccgc
     2041 gccgaatcca ggctgacctt cctggggctt gcggcactcg aggacccgcc ccgccccgat
     2101 gtggcagagg cggtcgcccg ttgccggggg gccgacatca ggataatcgt cgttacaggc
     2161 gatcacggcc tgaccgctgg ggccgtggcc cgcaaggtag ggattaccac cgggaaggcc
     2221 agggttgtga cggggcccga ggtcgacgcc atgtcccggg aagagctcga cactctgctg
     2281 cacgacacct ccgagctggt ggtggcgcgc tccaaccccg agacaaagct ccacgtggtc
     2341 gacgctttga gggcggaggg tcacacggtg gccatgaccg gcgacggggt gaacgacgcg
     2401 ccggccctga ggcgtgcgga cataggcgtg gcgatgggag actcggggac cgaggtcgcc
     2461 agggaggcgg caaccatggt gctaaccgat gattccttcg cctctatcgt ggcggctgtt
     2521 gaggaaggac gggtcgtcta cgacaacatc cgcaagttcg tgacctacat cttcgcgcac
     2581 gccaccccag aggtcgtgcc gttcttgatc tacgcgctct cgggcggggc agtgccgttg
     2641 cccctgaccg tgatgcagat cctggccata gacctgggga cggagacgtt gcccgccctc
     2701 gccctggggc gggagcccgc cgagcccggc gttatgagcc ggcgtccgcg cccgcgcggt
     2761 aaaggcatca tagacgggcc gatgctagcg cgggcgtggg gcaggctggg gctggtggag
     2821 gccgcgctcg tcaccggcgg gttcttctac gtgctgcacc aggcaggctg gtcgccgggg
     2881 gagccgacgg gtcctggtag cgcgcttcac ggggcctacc tcacggcgac cgccatgacc
     2941 ttcgccggga taacggcctg ccaggtcggg acggccttcg cggcccgcac gaaccacgcc
     3001 tccttgcgcg aaatcggggt cttctccaac aggctactcc tgtggggcat cctgttcgag
     3061 ctggtcttcg gcgcggctat cgtctacctg ccgccgctac aggcaacctt cggaaccgac
     3121 ccactgggcg ctcgcgagct ggccctgcta gccaccttcc cgttcgtggt gtggggaacc
     3181 gacgaactct ggcgttgggc ccgaaggagg agagacgcag gctagagggg acgcccccga
     3241 gggccgccat gcggccctcc cgcggagccg gtgatgcagt cactctaggc aagttgcgtc
     3301 agcggaatgg tcatgattgg tcatgccccc ttcgctggca agcgcgagac taagcgtgcg
     3361 atagggcgga tgtcccgacc ccctccccag gatgcccgcc cggtcgcaaa aagggagccc
     3421 ggaaggaaac ggtaaagcgt ggagatctct gtttatcgcg gttgggacga cgggtgggga
     3481 cggaccggag ccctggatca cccccgctgg cacgtggcat cgggagtcga tcactctggg
     3541 gcggcggtcc ggtcggaggt aggccaggca tgagggagag cgagatgggg ctcgggacgc
     3601 gctgggtcag gcatttttcg gaggtcggga tcgaagacgt cccgctcgtg gggggcaaga
     3661 acgcctcgct gggggagatg acgcgggagc tcgggacgct cggcatcagg gtacccgagg
     3721 gattcgccct gacggccgac gcctacaggc acttcctgga gtcgaacgaa ctggtctgtc
     3781 ctgttcgtga tgcactggac ggcttcgagc ccggcgacgt cgaggacctg ggacgcaaga
     3841 gccgcctcat ccgggacctc gtgcttggcg ccgagatacc gtccgaggtg aaagacgagg
     3901 tactccgcgg ctatgaggag ctctcatcac gctacgggga agactttacg gacgtggcgg
     3961 ttcgttcttc ggcgaccgcg gaggacctgc ccaccgcgag cttcgcggga cagcaggaga
     4021 gctacctgaa cgttcacggg gaggcgcagc ttctcgagtc ggtcaggaag tgcttcgcca
     4081 gcctct
//
LOCUS       DBNGVD010000520         5597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3413405, whole genome shotgun sequence.
ACCESSION   DBNGVD010000520 DBNGVD010000000
VERSION     DBNGVD010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3413405"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="AC16CM_14060"
     CDS             complement(<1..1208)
                     /locus_tag="AC16CM_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3141005.1"
                     /translation="MSQHRETLREILPIPDRPHTGLVTYDAKDPETRFPPIEPLRPPE
                     DAPNVLVVLLDDAGFGAASAFGGPCETVNFERLASGGLRYNRFHNTSLCAPTRAALLT
                     GRNHHSVGMGAITELATSAPGNNSLRPNTKAPLPETLRLNGYGTAQFGKCHEVPVFQN
                     SPVGPFDMWPTGSGFEHFYGFIGGEDSQWYPSLYEGTTPVEPPKTPEEGYHLTEDLAD
                     KAINWMRTQKALASDKPFFMYFAPGATHAPHHVPKEWIEKYKGHFAHGWDAQREITFA
                     RQKELGVIPQDAGLTERHEEIPAWDEMPEELKPVLERQMETYAAFLSHTDHHVGRLLD
                     SLEELGIMDETLIYVIIGDNGASAEGTLKGTFNEFIPLNGMGEALETNEFLIERIDEW
                     GGTEAYNHYAV"
     gene            complement(1531..1749)
                     /locus_tag="AC16CM_14065"
     CDS             complement(1531..1749)
                     /locus_tag="AC16CM_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141006.1"
                     /translation="MEKNEALKLAIEEAFVVPPTEASLRLQVTDLLLGSVRKVRMGRI
                     PLEQGDVMQRIEAALETYGSFFYPPEHR"
     gene            complement(1789..2340)
                     /locus_tag="AC16CM_14070"
     CDS             complement(1789..2340)
                     /locus_tag="AC16CM_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3141007.1"
                     /translation="MNPGPQIMSAIIFLTTPKALVADGWLSKVDSTNLNFSSLATNWE
                     IDLVVEDTEFASQMEELFENDISNNREIRLEGSGRRARARPQGRVDTRDTGARSGVVG
                     SGTGSGAALSRVGTTVFQKVTAPLQTHEHAMAAAVSRSLLGASLLGARFPRLIAWPVT
                     AVGAFLGGWHGARDPIHALRRQA"
     gene            complement(2371..2640)
                     /locus_tag="AC16CM_14075"
     CDS             complement(2371..2640)
                     /locus_tag="AC16CM_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141008.1"
                     /translation="MIHWRERTLYRVVVRSELIEVYAVAFEGMEMETKDGVTILTGEI
                     VDQPQLFGILDRLNGLGLELLSVEALPDSARSIARKERVPNGPKT"
     gene            complement(2895..5165)
                     /locus_tag="AC16CM_14080"
     CDS             complement(2895..5165)
                     /locus_tag="AC16CM_14080"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3141009.1"
                     /translation="MFIVQASGEPSGGSPMIVLKPKLRAPVPRPDQLVRRELLELLHD
                     ALECRVSVVSAPTGYGKTTLLAHWRRVEEADHPFAWVSLDEQDEDPIRLWRHVVEALR
                     RVVPEEDFGADVLVGMGAVRRGFVETTLPTLINELAELPHRIVLVLDDYQHVSAGDAN
                     GSVAYFLEHLPDNVHLVVSGRSDPPLPLGRLRARGELNEIRTRQLAFTEEEIACLLNE
                     RMGVGIEPDDISVLFERTEGWPAGIYLASLSLRDKEDKHAFIKSFGGSNRYIVGLLGE
                     EVLAGLDEEVRRFLLETSVLRRMTGPLCDAVTGRGGSASLLRGLANSNLFVVALDEQD
                     EWYRYHHLFSELLLYELRSAEPDLAPALRGRASDWLEGEGYVEGAIRQASEAGDYKHV
                     GLLIARHWYGYAFSGQTATVERWLASLPEGAIVRDAALCLVRAWLCALGGRPEESARF
                     LALAEGVSHEGPLPDGTASVESGAAVLRATFGFGGVTSAVEDAQRAARLEPERSPWAA
                     FVRFALGTGLYLSGETSLARKPLEGALELTAMRNQPLLEIAFQSWLSLVAADEGRSEE
                     AESRARAASALVDRFRLQAVPQANLASIALGHALAERGKPGEAREELERAYSARRRMP
                     GLSPWPMLVLLLTLAGVRAARGDRAGGRRALAEAREILEHNPDSGVFPELVERQERKL
                     RTRRPREGQLDVELTERELEVLRLLDGELTTRQMAERLYVAPSTVRTQVKSIYRKLGV
                     SSREQAVDEARNRGLI"
BASE COUNT          891 a         1899 c         1769 g         1038 t
ORIGIN      
        1 accgcgtagt ggttgtacgc ttccgtcccg ccccactcgt cgatgcgctc gatcaggaac
       61 tcgttcgtct cgagggcttc gcccatgccg ttgagcggga tgaactcgtt gaacgtcccc
      121 ttgagcgtgc cctcggcaga agccccgttg tcgccgatga tcacgtagat gagggtctcg
      181 tccatgatcc cgagctcctc gagcgagtcg agcaacctcc ccacgtgatg atcggtgtgc
      241 gagaggaacg cagcgtaggt ctccatctgg cgctcgagga cgggcttgag ttcctccggc
      301 atctcgtccc aggcggggat ctcctcgtgc cgctcggtca gccccgcgtc ctgcgggatg
      361 acgccaagct ctttttgcct ggcgaaggtg atctcgcgct gcgcgtccca gccgtgggcg
      421 aagtggccct tgtacttctc gatccactct ttaggtacgt ggtgcggcgc gtgcgtcgca
      481 cctggcgcga agtacataaa gaacggtttg tccgacgcca gcgctttctg ggtgcgcatc
      541 cagttgatcg ccttgtcagc aaggtcctcg gtgaggtggt agccctcctc aggggttttt
      601 ggcggctcga ccggtgtcgt accctcgtag agcgagggat accactggct gtcctcgccg
      661 ccgatgaacc cgtagaagtg ttcgaacccc gagccggtcg gccacatgtc gaacggaccg
      721 accgggctgt tctggaatac cggcacctcg tggcacttgc caaactgcgc ggtcccgtag
      781 ccgttcagcc gcaacgtctc gggtagcggc gccttcgtgt tcggccgcag cgagttgtta
      841 ccaggcgccg atgtggcgag ctccgtgatg gcgcccatgc cgaccgaatg gtggttgcgg
      901 ccggtcaaaa gcgccgccct tgttggagcg cacagggacg tgttgtggaa tcggttgtag
      961 cggaggccgc cgctggcgag ccgctcgaag ttcaccgtct cgcacggccc accgaaggcg
     1021 cttgcggcgc caaagcccgc gtcgtcgagc aggacgacca gcacgttcgg cgcatcctcc
     1081 ggcggcctga gcggctcgat cggcgggaag cgggtctccg ggtccttcgc gtcgtaggtg
     1141 acaaggcccg tgtgaggccg atccgggatg ggcaggatct cccttagggt ttctcggtgc
     1201 tgggacaatg tacgtcctcc ttctgatggc ttgtctttgt ctcgcgcccc gggcgcggct
     1261 acgggcaccg gcttcttccc gatcgcggcg gcgagacggg atggatctcc gagctgcagg
     1321 ccgggcaccg gtacacgccg tctgggagcc gtcgtatctc tgggtgcccg cccccgctcg
     1381 tcccgcttcc tgtagccagc cggcatcggt cgcacgggat ggggggttct ggtcttgctc
     1441 gactttcatg ccatctcctt catggcatcg ggatactgca cgtgtcgaac agggaggtcc
     1501 tgtccggcac gcggttcggc gaccggttcg tcagcggtgc tcgggtgggt agaagaagct
     1561 cccatacgtt tccagcgccg cttcgattcg ttgcatgaca tcgccttgct ccaggggtat
     1621 acgccccatc cgaaccttgc gcacgctgcc caggagcagg tcggtaacct gcaaccgcaa
     1681 cgacgcctcg gtcggaggga ccacgaacgc ttcctcgatg gctagtttta gagcctcgtt
     1741 cttttccata tcgtccgtcc tcctctcgat tgcctcggac tcggaacgtc aggcttgccg
     1801 tctgagagcg tggataggat cgcgcgcacc atgccaaccc ccgaggaagg cgcccaccgc
     1861 cgtaacgggc caggcgatca gacgcgggaa acgcgcgccc agcagcgacg ccccgagaag
     1921 ggaccggctc accgccgcgg ccatggcgtg ctcgtgggtc tgcagggggg ctgtgacctt
     1981 ctggaaaacg gtcgtgccaa ccctggaaag cgcggcccca gagcccgtgc cgctgcccac
     2041 cacgccggag cgcgcaccgg tgtctcgggt atccacccgg ccttgtggtc gcgccctcgc
     2101 tcgtctccca gagccctcca ggcggatctc cctgttgttg gagatgtcgt tctcgaagag
     2161 ctcttccatc tgggaggcga attcggtgtc ctccaccacc aggtctatct cccagttggt
     2221 ggccaggctg ctgaagttca ggttcgtcga gtccaccttc gacaaccagc cgtcggccac
     2281 gagcgccttg ggagtggtga ggaagatgat cgccgacatg atctggggtc ccgggttcaa
     2341 gggtaatggc gagaggaagg acggccaggc tcatgttttc ggtccatttg ggacccgttc
     2401 tttccgagcg atcgagcggg cgctgtccgg cagggcctca acactcagca gctccagccc
     2461 cagaccgttc aagcgatcga gtatgccaaa cagttgcggc tggtcgacga tctcacccgt
     2521 gaggatcgta accccgtctt tcgtctccat ctccatccct tcgaaagcca cggcgtacac
     2581 ctcgatcagc tcgcttcgaa cgactacccg gtagagcgtc ctttcgcgcc agtgaatcac
     2641 cgcctaccgc aactcctgtt ggtgctcttt cgtctctttt acgccgccga gcgtagtgca
     2701 aggtaggcac gtgagtcatc acccacaggg atgtttcccc taggggatgc gctattgtca
     2761 cgcatgccag agcagcaatt ggtgccggca gcgcaaccgt gccgccaccg ccgccatgtg
     2821 cgaatacgca cggcttcttc gcgcgtgggc cgcggagcat cccccggcga ggcacctcca
     2881 gaccgcgaat cgatctagat aagtcccctg ttgcgtgcct catccactgc ttgctcgcgc
     2941 gaggagacgc ccagcttgcg gtagatcgac ttcacctggg tcctcaccgt gctcggggcg
     3001 acgtatagtc tctcggccat ctgccgggtg gtgagctccc catctagcaa gcggagcacc
     3061 tcgagctcgc gctccgtgag ctcgacgtcg agctgcccct cccgcggcct gcgcgtgcgg
     3121 agcttgcgtt cctggcgctc cacgagttcg gggaacactc cggagtcggg attgtgctcc
     3181 aggatctccc gcgcctcggc gagcgcccgt ctgcccccgg cccggtcgcc gcgcgcggcc
     3241 cgtaccccgg ccagcgtcag cagcaggacg agcatgggcc aggggctcaa gccaggcatc
     3301 cttcgtcgcg cggaataagc cctctccaac tcctccctgg cctcccccgg tttaccccgt
     3361 tcggcgagcg catggcccag cgcgatggat gccaggttgg cctggggtac tgcctgcaac
     3421 cggaacctgt ccacgagcgc gctcgcggcc ctcgcgcgcg actcggcctc ctccgaacgg
     3481 ccctcgtccg cggcgaccag cgacagccag gattggaagg ctatctccaa caagggctga
     3541 tttctcatcg ccgtcagttc tagggcccct tcgagcggct ttcgggccag cgacgtctcc
     3601 ccggagaggt agagaccggt tcccagagcg aagcgaacga acgcggccca cggcgagcgt
     3661 tcgggctcga gccgtgcggc gcgttgggcg tcctccaccg cgctcgtgac gccgccgaac
     3721 ccgaaggtgg cccgcaggac ggccgcgccc gattcgaccg aggcggttcc gtccgggagc
     3781 gggccctcgt gggagacgcc ctccgcgagc gccaagaatc tggcgctctc ctccggccgg
     3841 ccgccgagcg cgcagagcca ggccctgacc aggcagagcg ccgcgtctcg gacgatcgcc
     3901 ccctcaggca gcgaggcgag ccaccgctcg acggtcgccg tctggccgga gaacgcgtag
     3961 ccgtaccagt gacgcgcgat caggagcccc acatgcttgt agtcaccggc ctcgctcgcc
     4021 tgccggatcg cgccctcgac gtagccctcg ccttcgagcc aatcgctcgc tctcccgcga
     4081 agggcgggtg ccaggtcggg ttcggcgctc ctgagctcgt agagcaagag ctcggagaag
     4141 aggtggtggt agcggtacca ttcgtcctgc tcgtctagag cgaccacgaa aagattggaa
     4201 ttggcgagcc cgcgcagcag gctggcggag cctcccctgc ccgtcaccgc gtcgcacagc
     4261 gggcccgtca tcctcctcag caccgaggtc tccagcagga aacgcctgac ttcctcgtcg
     4321 agaccggcga ggacctcctc ccccaggagg ccgacgatgt agcggttgga gcccccgaac
     4381 gacttgatga aggcgtgctt gtcctccttg tcccgcaaag agagggaggc taggtagatg
     4441 cccgcgggcc acccctcagt gcgctcgaag agcacggaga tgtcgtcagg ctcgatgccc
     4501 acgcccatcc tctcgttcag caggcaggct atttcctcct ctgtgaaggc gagctgtctg
     4561 gtgcggattt cgttcaattc tcccctggcc ctgagcctcc ccaacggcag cggcgggtcg
     4621 gaacgaccgg agacgacgag gtggacgtta tcagggaggt gctccaggaa gtatgccacc
     4681 gacccgttgg cgtctccggc gctcacgtgc tggtagtcgt cgagcaccag cacgatccga
     4741 tggggcaact ccgcgagctc gtttatgagg gtgggaagcg tcgtttctac gaatccccgc
     4801 ctcacggcgc ccatccccac gagaacgtcg gccccgaaat cctcttccgg cacaacccgc
     4861 cgcaacgcct ccaccacgtg tctccaaagc cggatcggat cttcgtcttg ctcatctagg
     4921 gaaacccacg cgaagggatg gtcggcttct tctacccgac gccagtgggc caaaagcgtg
     4981 gtcttgccgt agccggtggg ggcgcttacg acgctaaccc tgcactccag ggcgtcgtgg
     5041 agcagttcga gcagttcacg tcgtaccagc tgatcggggc gcgggaccgg cgccctcaac
     5101 ttcggtttga ggacgatcat gggggaaccg ccgcttggct cgcccgaggc ctgcacgatg
     5161 aacacctccc tcctgcgcca cccctatctg gggccgcacc caccgagcga tcgccgagac
     5221 accgccagcc cgttggcgcg gggacctcct tgcttttggt ccgatgcgcc cgacgccctc
     5281 ggcaggccag aacttttctc ttcgatcacc cgttctcgac cggaacccgg cggttccgac
     5341 gcgtgcgtat tttctcacaa ccggcggcga aactgaaatc cggcgatgcg atctcgcacg
     5401 atggatccca agctcacttt tcgctggcgg ggcgcgtagc ctttagaaca catcgtgcat
     5461 cttgcgcaac cgcacgcggg ccgcgaagct cggggagccg aaggggtttc ctgggggcac
     5521 gcagggccga cgattccgtt gcgggcgcag gtgcgcggcg ggatatcccc tagtgcatct
     5581 ccagaatagg tgtcgtg
//
LOCUS       DBNGVD010000521         5633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_500320, whole genome shotgun sequence.
ACCESSION   DBNGVD010000521 DBNGVD010000000
VERSION     DBNGVD010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_500320"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16CM_14085"
     CDS             complement(<1..221)
                     /locus_tag="AC16CM_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141010.1"
                     /translation="MNADGTGQRQLTSELSSSPAWSVPVAGAPDGRIAFVRGGKIWTM
                     LADGTGEAALSYDCPTENGGFCDNAVGNPT"
     gene            complement(372..3458)
                     /locus_tag="AC16CM_14090"
     CDS             complement(372..3458)
                     /locus_tag="AC16CM_14090"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3141011.1"
                     /translation="MRISLLGRFEVSVGSTLISEDGWRLRKAASVVKLLALAPDHSLH
                     REQVMNLLWPNLGTRAAANNLHQSLHIARRTLEPGAKTYAYLHMRGQHLVLCPRDSAW
                     VDVKAFESAAREAWNSRQPAAYRAAVDLYSGDLLPRDRYEEWAERRREELLGTYLALL
                     LRMAALLEEMEDFDSAAAALREVVAREQTNEQAHVTLMRVYARAGQRHRAVRQYEHLK
                     DALSRDLGVEPQAASHHVYEKILSGHVPVPPPTDAECQPEAGKHNLTGSLTSFVGRER
                     ERREVKQLLETSRLLTIMGAGGSGKTRLAQEVARDLMDAYANGVWLAELAAVAEGSLV
                     GGAIAEVLGVPEQPGRPIEATLVDALREKHILLVLDNCEHLVNDVARLAELLLRSCPM
                     LRILATSREILGASGEVFWQAPPLSGPDPASANTIEELEAFESVRLFIQRARYRTPTF
                     TLTRQNAAAVAEICARLEGIPLAIELAAARVGLSVKEVADRLDDSLNLLTTGNRTAVP
                     RQRTLRGALDWSHDLLSGPERALFRRLSVFAGGWTLRAAEAVGSGDGIEEGRVLDLLS
                     QLVDKSLVLANTTGGGTVRYRLLEPVRQYARERLQESEESKAVQLRHACWCLELVEEA
                     LEGLRGPEQASWVGLMQEEHANAQAALSWSLESEPGTALRLAATLGYFWYRYGHIIEG
                     RHWLETVLARTDGNETATRAKTLRLAGVLSEESGLYVRAEKLHEQGLALYRRLGNREG
                     IAASLTSLGALAYAVGDLERAMSLTQESLVLKRELGDERALMSSRNNLGEMMQKAGEL
                     EEAQSLFEENLRSDETSKDEWGAAVSRLNLGILAIEQGEPLRSEKLLFEAIRVLRRLR
                     DDDASTECLESLAAAAGARGERDRAATLFGAAEAAREKLGVPIRPVDRERYERFIAQS
                     RCGQNEKAWASAWSRGRNLSLQSAIDQALSPVKGMTTSRRGSERPSLLTTREREVAHL
                     VAQGLTNRLISEELSISERTVSTHVGGIFNKLELRSRSQLATWVAGQRDSDSERIL"
     gene            complement(3615..4958)
                     /locus_tag="AC16CM_14095"
     CDS             complement(3615..4958)
                     /locus_tag="AC16CM_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141012.1"
                     /translation="MRDSLKALRTLLGVVFLAGYAWQVFVPGSLGTAIFLGVGAVLFA
                     ISVPWSSGFHKGFTLVAFAAFGATILFGRFRFEDYYQGMPTYFGIVAVLLVLSVAGYP
                     IKAARYEAQIRALMAALTHRGVGVKTTSGALGHVLGSVLDVGSLVLVDVITGRSAPKD
                     RPDALKWAGRAYSFVPLWTNLNLLTATTIVLTGVSYLSMLSVTLPFAFVGLAATLFFA
                     QREGGAGEEIPRAPLDRGAAAVLLYPVLLVSAVALVNISFPVLPLTAAIAITVVVIVT
                     LIATLATTLTRKASPLARLGRETRESLQTSHPEFTLFGSAGILVLSLNELGALRPLGT
                     FLSALPTTLVAPALAMIMAVGFVAGIHVIPMVLLLNTAFPLDGGPAPALWAAAILLGS
                     QAVLLLTPFSTTVTMLSRLLGQHPIEVGPRRNLWFGIAVSLGATLYLGLLTLLLL"
     gene            complement(4971..>5633)
                     /locus_tag="AC16CM_14100"
     CDS             complement(4971..>5633)
                     /locus_tag="AC16CM_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR3141013.1"
                     /translation="ETVVGRALVLADSVGLEGVTIRRLAKDLGVTPMALYWHFRNKDA
                     LLDAMMGRIFAEVDPALEDSATWVEQFRALMRSLAGALRAHPGVAPLFATRTNSSESS
                     LRFTEVALDVLRRGGFSPVEATRVSRHALSTIVNLVVGEPGFISPEGSDEPLDARRRA
                     RLYLETLPPERYPRLVEAAVPLSAPEDPEAYYQFGLDLLLAGVEEMAARKAREPARGV
                     RR"
BASE COUNT          999 a         1851 c         1776 g         1007 t
ORIGIN      
        1 gtggggttgc ccacggcgtt gtcgcagaac ccgccgttct cggtcgggca gtcgtaggac
       61 agcgcggctt cccctgtgcc atccgcgagc atggtccata tctttcctcc gcgcacgaag
      121 gctatcctgc cgtcgggagc gccggccaca ggaacggacc aggccgggct tgagctcaac
      181 tccgatgtta gctgccgctg gcccgtgccg tcggcgttca tggaccatat gttgtagacg
      241 ttgtctgtgc cccgtcgctg gaacgtgagc ctggagccgt cgtgcgacca tgccgggttc
      301 ccatccagga gcgggctatt ggtgaggttg cgcggcgtcc ctgagccgtc ggcattggat
      361 attagaggcc gttacaggat tctctccgag tcggagtctc tctgcccggc aacccaggtg
      421 gcgagttggg acctggatct aagctccagt ttattgaaga tcccgccaac gtgagtcgag
      481 accgtacgtt cggagatgga gagctcttcg gagatgaggc gattggtaag accctgggcg
      541 acaagatgcg cgacctctct ctcgcgtgtg gtcagtaggg aaggacgttc cgacccccgt
      601 ctgcttgtag tcatgccctt gacgggcgaa agcgcctggt cgatggcact ttgcagtgaa
      661 aggttccggc ccctcgacca cgcagacgcc catgccttct cattctggcc acaccgggat
      721 tgcgcaatga agcgttcgta cctctcccgg tccacaggcc gtatcggaac tcctagcttt
      781 tcgcgcgcgg cctcagcggc cccgaagagc gtcgccgccc tgtccctctc acctcgcgct
      841 ccagcggccg cggccagaga ttctaggcac tccgtagatg cgtcatcgtc cctgagtcgc
      901 cgaagtaccc ttatcgcttc gaacagcaac ttctccgatc ggaggggctc tccctgctcg
      961 atggccagga ttcccaggtt caggcggctg acggccgcac cccattcgtc tttcgaagtt
     1021 tcgtcgcttc gcaggttctc ctcgaacaac gactgggcct cttccaactc gcccgctttc
     1081 tgcatcatct cgccgaggtt gtttctggag gacatgagag ccctctcgtc gcccagttcc
     1141 cgcttgagca cgagcgactc ctgggtcagc gacatcgcgc gctccaggtc gcccacggcg
     1201 taagccagag caccgaggct ggtgagagag gccgcgatgc cctctctgtt tcccagtcgc
     1261 ctgtacagtg ccagcccttg ctcgtgtagc ttctcggccc gtacgtagag cccgctctct
     1321 tccgagagga caccggcgag gcgaagcgtt tttgcccggg tggcggtctc gttaccgtcc
     1381 gttcgcgcca gcacggtctc caaccagtga cgcccctcga tgatgtgccc gtagcggtac
     1441 cagaagtatc ccagggtggc tgcaagccgc agtgcggtcc caggttcaga ttcgagcgac
     1501 catgagagcg ccgcctgcgc gttagcatgt tcttcttgca tgagcccgac ccacgaggct
     1561 tgctcgggac cgcgaagtcc ctccaatgct tcctcgacca actccaggca ccagcaggcg
     1621 tgacgaagtt gcacggcctt cgactcttcg ctctcctgga gcctctcccg agcgtactgg
     1681 cgcacgggtt cgagcagccg gtagcggact gtgccgcccc ctgtcgtgtt cgccagcacc
     1741 agggatttgt ccacgagttg cgaaagtaga tccagcaccc taccctcttc gatgccgtct
     1801 cccgacccca cggcctcggc cgcccgcaac gtccagcccc cggcgaacac ggagagtcgc
     1861 cggaacagcg ctcgctcggg cccggagagc aggtcgtggc tccagtcaag ggcccccctg
     1921 agcgttctct gcctcggtac ggcggtccgg ttacccgtcg tcaacaggtt caaagaatca
     1981 tcgagcctgt cggcgacctc cttgacggag aggcccaccc tcgctgcggc gagctcgatg
     2041 gcgagaggta tgccttccaa cctcgcgcag atctccgcca cggccgccgc gttctgccgg
     2101 gttagggtga aggtgggggt gcggtagcga gcccgctgga taaacagacg caccgactcg
     2161 aacgcctcga gttcttcgat cgtgttcgcg ctggcaggat cgggtcccga gagcggcggc
     2221 gcctgccaga atacctcccc ggaggcgccc aggatctcgc ggctggtcgc gaggatcctg
     2281 agcatcgggc acgatctgag gaggagttcg gcaaggcgtg ccacgtcgtt caccaggtgc
     2341 tcgcagttgt ccaggacgag caggatgtgc ttttcgcgca gggcatcgac gagcgtcgct
     2401 tcgattgggc ggccaggctg ttcgggtacc cccaggacct cggctatcgc ccctccgacc
     2461 agagagcctt cggcgaccgc cgccaattcc gcgagccaga caccgttcgc gtacgcgtcc
     2521 atgaggtccc tggcgacctc ttgtgcgagg cgagtcttgc ccgagccccc cgcgcccatg
     2581 atggtgagca ggcggctggt ctctagtaat tgcttgacct cccgtctctc tcgttcccgt
     2641 ccgacgaagc tggtcagaga gcccgtaagg ttgtgcttgc cggcttcggg ttgacactcc
     2701 gcgtcggtgg gcggcgggac ggggacatga ccagacagta tcttctcgta gacgtggtgg
     2761 cttgccgcct ggggttctac accaaggtca cgtgagagcg cgtccttgag atgctcgtac
     2821 tggcgtacgg ctctgtgacg ttggccggcc ctggcgtaga cccgcatcag cgttacgtgc
     2881 gcctgctcgt tcgtctgttc acgtgccacg acctcccgga gtgccgccgc tgccgaatcg
     2941 aagtcctcca tttcctcgag tagcgcggcc atcctgagga gaagtgccag gtacgttcct
     3001 agcaattctt ccctgcgtcg ttctgcccac tcctcgtagc ggtcgcgtgg caacaggtcg
     3061 cccgagtaca gatcgaccgc cgccctgtaa gccgcgggtt gccgggagtt ccaggcctca
     3121 cgcgcggcac tctcgaaagc cttgacgtcc acccaggccg aatcccgggg acacaaaacg
     3181 agatgttgcc ctcgcatgtg tagataggcg taagtcttgg ccccgggttc gagggttctt
     3241 cgcgcgatgt gcagtgactg gtgcaggttg ttggccgcgg ccctggtccc aaggttgggc
     3301 cagagcaggt tcatgacctg ctcccgatgg aggctgtgat ccggggccag agcgagaagc
     3361 ttcacgacgc tcgcggcctt tctgagtcgc cacccatcct cgcttataag ggtcgaacca
     3421 acagacacct cgaagcggcc gagcaaggat attcgcaatc ctgttcgact atcagctacc
     3481 agatcaccct tcgcttgcaa cgttatcgat gcgccccggc gagatcctct atcgtccagc
     3541 gctccctaca cccatcatca taatagcaag gcgggtgcca ctgcgtcttg cgcgggaggc
     3601 tcccgcccga ctacctacaa gagcaagagc gttagcaatc ccaggtatag cgtggcgccc
     3661 agagacacgg cgatgccaaa ccacaagttg cgcctgggac cgacctctat cggatgctgc
     3721 ccaagcagcc gcgagagcat ggtgacggtt gttgagaacg gggtgagaag caggacggcc
     3781 tgggagccga gcaggatggc cgctgcccac aacgccggcg ctggcccacc gtcaagcgga
     3841 aaggcggtgt tgagcagcag caccatcggt atgacgtgta tgccggcgac gaacccgact
     3901 gccatgatca tcgcgagcgc cggcgcgacc agtgtggtgg ggagagcgga gagaaacgtg
     3961 cctagcggtc gcagggcacc gagctcgttc agggagagga cgaggatgcc ggctgagccg
     4021 aagagggtga actcggggtg ggatgtctga agcgattccc tggtctcgcg cccgagacgt
     4081 gccagcggtg aggccttgcg ggttagcgtc gtggcaagcg tcgcgatgag cgtcacgatg
     4141 accacgaccg tgatggcgat agccgccgta agcggcagga cggggaacga gatgttgacc
     4201 agcgcgacgg cggagacaag cagcacggga tagagtagaa cggccgccgc gccccgatcc
     4261 agcggcgcgc gtgggatctc ctcgcccgcg cctccttctc tctgggcgaa gaacagggtc
     4321 gccgcgagcc cgacgaacgc gaagggcagc gtcacggaga gcatgctcag gtaggagacg
     4381 ccggtcagga cgatggtggt cgcggtcagg aggttcaggt tggtccacag gggcacgaac
     4441 gagtaggccc gtcccgccca tttcagggcg tcgggcctgt ctttgggggc tgagcggccc
     4501 gtgatcacat ccaccagcac gagcgatccc acgtcgagca cggacccgag gacgtgaccg
     4561 agcgcccccg aggtcgtctt gacgccgacg ccccggtgcg tgagcgcagc catgagagcc
     4621 cggatctgag cctcgtaacg cgcggctttt atgggataac ccgccacgct gagcacgagc
     4681 agtacggcca cgatcccgaa gtacgtgggc attccctggt agtagtcctc gaaccggaag
     4741 cgtccgaaga gaatggtggc accgaaggcg gcgaacgcta ccagggtgaa ccccttgtgg
     4801 aagccgctcg accatggcac ggagatcgcg aacaatacgg caccgacccc caggaatata
     4861 gcggttccca aagaaccggg tacgaagacc tgccaggcat agccggccag gaacacgacg
     4921 cctagcaagg tacgcagagc tttcagacta tccctcacgc ggtccccgat ctatcgccgc
     4981 acgcccctgg caggctcgcg cgccttgcgt gcggccatct cctcgacgcc tgccaggagg
     5041 agatccaggc cgaactggta gtaggcctcg ggatcctcgg gggcgctcaa gggaaccgcc
     5101 gcctccacga gccgcggata acgctcgggc ggcaacgttt cgaggtagag cctcgcccgg
     5161 cgcctggcat ctaacggctc atccgagccc tcgggcgata tgaacccggg ctcccccacc
     5221 accaggttga ctattgtgct caacgcgtgc cgggataccc gcgtggcctc gacgggggag
     5281 aagccaccgc gccgcagcac gtccagggca acctcggtga aacggaggct gctctccgaa
     5341 gagttggttc tggtcgcgaa gagcggcgcg accccggggt gcgcccgcaa cgccccggca
     5401 agcgaccgca tgagcgccct gaactgctcc acccacgtcg ccgaatcctc gagggcagga
     5461 tccacctccg cgaagatcct gcccatcatg gcgtcgagca acgcgtcctt gttgcggaag
     5521 tgccagtaaa gggccatcgg cgttacgccg aggtccttcg ctaacctcct gatggtcacc
     5581 ccctccaacc ccacagaatc cgcgagcacg agcgcccgcc ccactaccgt ctc
//
LOCUS       DBNGVD010000522         3792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2138802, whole genome shotgun sequence.
ACCESSION   DBNGVD010000522 DBNGVD010000000
VERSION     DBNGVD010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2138802"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC16CM_14105"
     CDS             complement(<1..395)
                     /locus_tag="AC16CM_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141014.1"
                     /translation="MKEISKGGGLRTSRRSDGVKVRTVRRTPVNKKVSYARRRQVAGL
                     VLIGCVLLLGVVLFAFIGDDDEIAGGVSIGSVDVGGMTRAEAVKSVQQDASATFREIS
                     FGTGKDGFSVSGDDLGVEVNAAQAVDEAYT"
     gene            739..1941
                     /locus_tag="AC16CM_14110"
     CDS             739..1941
                     /locus_tag="AC16CM_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141015.1"
                     /translation="MKTTVQLVKGSEMEDRIAQLRILSYPDFPEVHDVGYYSRLYRWY
                     QDHPLADELYRWVSVTEEGEVVGHLTAFPQYYRIDGRRVVAHTPGDYMVLGQHGFQAI
                     MLMRSFFRQTENCVVCDMVPAVIGVETRLGAGVVGDLKYAAKLMNVSRLPMPSVPAPL
                     RRVFRLPETFAPARGFTDRPGADPQDAEEHVAPPAPRPRAPIPRPVQKLLNRGLERMD
                     EALSRGFGAGLEAEELDGFDDDFDDLFEKIAVVVPCTAEKDAAFLRWRYGGGSPQAPV
                     TLLGVRSGGRLLGYAVLRTAFHGQDGYILDLTALPGRQDVIRALLRETVRSFRRMGVQ
                     IIRYRFIESPTAPWPADLQRLGFFRRRGRSHKLLTRFKDEHLRETTDELTNWSMSFGD
                     GEAGFWLR"
     gene            1956..2207
                     /locus_tag="AC16CM_14115"
     CDS             1956..2207
                     /locus_tag="AC16CM_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR3141016.1"
                     /translation="MVSGNGDARAAIRGWLQENVTGGRDVPNDEPLIDNGVMTSLQTV
                     ELVVFLEDAFGIVVEDEEFDEENFGSVDAIATLVESKAA"
     gene            2204..3790
                     /locus_tag="AC16CM_14120"
     CDS             2204..3790
                     /locus_tag="AC16CM_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3141017.1"
                     /translation="MSVSPEYTLHDLLHNSVARDPSKAAVVDGDTRYTYEDLERESSA
                     LGAALVGSGVVRGDRVGVLLEKSWEAIVAMLAASRIGAAYVNVNPLFKAPQVEYLADD
                     CDIKVMIGDSRKLDELRPKTVETAFYRGADPEGASAASYVDLAEAMKGDGLVEDRKVS
                     EADLATILYTSGSTGMPKGVATSQRNVVVGAQIVSTYLENTPEDRILSALPLNFDAGM
                     SQFTTSLRAGATLYLLRSRLPGDLLKALRRHEITGVTGVPPLWALLIRGARSLRENPL
                     THLRYLANTGGRIPQANLDELRALLEPAGTRIFLMYGLTEAFRSTYLAPEEIWRSSPE
                     QGNCIGKAIPNTDIMVVTKEGREAAPGEPGELVHRGPTVALGYWGNEEATRKAYRPNP
                     LAPPELLEVERVVYSGDTVKRDDEGYLYFLGREDAMIKNQGYRLSPEEVENLLLGSGF
                     VHEACAFGVENPAVGQDVIAVISLREGGEGTVDKVREYVIANGPPYMVPKEILVRDEL
                     PKTGSGKIDRKGISNAYAIG"
BASE COUNT          699 a         1232 c         1254 g          607 t
ORIGIN      
        1 gtgtaggcct catccaccgc ctgggccgcg ttcacctcga cgccgaggtc atcgccgctc
       61 acggagaacc cgtccttgcc ggtgccgaag gaaatctccc ggaaggttgc cgaggcgtcc
      121 tgctgcacgc tcttcacggc ctcggctctg gtcatgccgc ccacgtccac agagccgatg
      181 ctgacgccgc cggcgatctc gtcatcatcg ccgatgaagg cgaacaggac gaccccgagc
      241 agcaggacac agccgatcaa gaccaggcca gccacctgac gcctgcgggc gtagctgacc
      301 ttcttgttta cgggcgtgcg tcgcaccgtc cggaccttca ccccgtccga acgccggctg
      361 gtccgcagac cgcctccctt gctaatctct ttcaaaaagt cgcctctcct gcgataccag
      421 ttagcgccac cagccccaag aggaaccctg tatagtcgcg gcggcacaca ggcgctatta
      481 ttacggcaag cgcccgaacg ttcaacctct cggaaagatt gactcccgaa tacgcctcgc
      541 ctgacatcgt ggtaggcagg ataccagccc ggcccgggaa ccccataaga gatggcccgc
      601 accgagcttg gtttttgcgg gaccctccat tacactctgc cttttggatc tgcaccaaaa
      661 acactacgga gtgagaggtt ccttgcgcca cgcatcacac agagcgagac acggagcgcg
      721 gcagccgggg tgccggccat gaagacgacc gtgcaactcg tgaagggctc ggagatggag
      781 gacaggatcg cgcagctgcg catcctctcc tacccggatt tccccgaggt ccacgacgtg
      841 ggctactact cccgcctcta ccgctggtat caggaccatc ctctggcgga cgagctgtac
      901 cgctgggtct ccgtgacgga agaaggggag gtggtcgggc acctcacggc gttcccgcag
      961 tactacagga tcgacggccg acgggtggtg gcccacacac cgggcgacta catggtgctg
     1021 ggccagcacg ggttccaggc catcatgctg atgcgctcct tcttccggca gacggagaac
     1081 tgcgtggtct gcgacatggt gccggcggtt ataggcgtgg agacccgcct gggcgccggg
     1141 gtcgttggcg acctgaagta cgcggcgaag ctgatgaacg tctcgcggtt gcccatgccc
     1201 tcggttcccg caccgctcag gagggtgttc cggcttccgg agacattcgc gccggcccgc
     1261 gggttcaccg acaggccggg cgccgatccg caggacgcag aggagcacgt cgcgccgccg
     1321 gccccgcgcc cgagggcccc gataccgcgc cccgtccaga agctgctgaa ccgggggctc
     1381 gagaggatgg acgaggcgct gagccgcggc ttcggagcgg gcctggaggc cgaggagctc
     1441 gacgggttcg acgacgactt cgacgacctg ttcgaaaaga tcgccgtcgt ggtgccctgc
     1501 acggcggaga aggacgctgc gttcctgcgc tggcgctacg ggggcggctc tccccaggca
     1561 cccgtaacgt tgctcggcgt caggtcgggg ggcaggctgc tcggctacgc cgtgctcagg
     1621 accgccttcc acggccagga cggctacata ctcgacctga cggcgctccc gggacgccag
     1681 gatgtgatcc gggcgctctt gcgcgagacg gtgcgctctt tcaggcggat gggtgtgcag
     1741 ataattaggt acaggttcat cgagtcgcca accgcgccgt ggccagccga cttgcagcgg
     1801 ctcggcttct tccgccgcag ggggagaagc cacaagctgc tcaccaggtt caaggacgag
     1861 cacctgcgcg agacgacgga cgagctcacc aactggtcga tgtctttcgg cgacggcgaa
     1921 gcgggcttct ggctcagata gaagaggagg cttacatggt ttcaggcaac ggagacgcga
     1981 gggctgccat acgcgggtgg ctgcaggaga acgtgaccgg cgggagggac gtcccaaacg
     2041 acgagcccct catcgacaac ggcgtgatga cgtcgctcca gaccgtcgag ctggtggtgt
     2101 ttctggagga tgccttcggc atcgtcgtcg aggatgagga gttcgacgag gagaacttcg
     2161 gctccgtcga cgccatcgcg acgcttgtgg agagcaaagc ggcgtgagcg tcagccccga
     2221 gtacacgctc cacgacctgc tccacaactc cgtggcgcgg gacccgtcca aagcggcggt
     2281 cgtggacggt gacacccggt acacctacga ggacctggag cgtgagagct cggcgcttgg
     2341 cgccgccctc gtaggctccg gcgtagtgcg gggcgacagg gtaggcgttc tcctcgagaa
     2401 gtcctgggag gccatcgtcg ccatgctcgc cgcgtcgcgc atcggggccg cctacgtcaa
     2461 cgtgaacccc ctcttcaagg cgcctcaggt cgagtacctc gcggacgact gcgacataaa
     2521 ggtcatgatc ggggactccc gcaagctcga tgagctccgc ccgaagaccg tcgagaccgc
     2581 tttctaccga ggcgcggatc cggagggagc tagcgccgcc tcctacgtcg acctcgcgga
     2641 ggcgatgaag ggcgacgggc tcgtggagga caggaaggtc tccgaggctg atctcgcgac
     2701 catcctctac acctcggggt ctacggggat gcccaagggc gtggcgacca gccagcgcaa
     2761 cgtggtggtc ggggcccaga tcgtctctac ctacctggag aacaccccag aggaccgcat
     2821 actctcggcg ctccccctca acttcgacgc agggatgagc cagttcacga cctcgctcag
     2881 ggcgggggcc accctgtatc tgctccgctc caggcttccg ggggatctcc tcaaggcgct
     2941 caggcggcac gagatcacgg gcgtaaccgg cgtgccgccg ttgtgggcgc tcctgatccg
     3001 gggtgcaaga tcgcttaggg agaaccccct gacgcacctg cgctacctcg ccaacacggg
     3061 gggccgcata ccacaggcca acctcgacga gctgagggcc ttgctcgagc cggcaggcac
     3121 caggatcttc ctgatgtacg ggctcacgga ggccttccgc tccacgtatc ttgcgccgga
     3181 ggagatctgg cgttcctccc ccgaacaggg caactgcatc ggaaaggcca tcccgaacac
     3241 cgatatcatg gtggttacca aggagggccg agaggccgcc cccggcgagc cgggcgagct
     3301 ggtccaccgc ggtcccaccg tggcccttgg ctactggggc aacgaggagg caacaaggaa
     3361 ggcgtaccgt ccaaacccgc tggccccgcc ggagctcctt gaggtggagc gagtcgtcta
     3421 ctcgggtgac accgtgaagc gggacgatga gggataccta tactttctgg ggcgcgagga
     3481 cgccatgatc aagaaccagg gctaccgcct gagccccgag gaggtcgaga acctgctgct
     3541 cggctcgggg tttgtgcacg aggcctgcgc cttcggcgtc gagaaccctg ccgtcgggca
     3601 ggacgtgata gcggtgatct cgcttcgcga aggcggcgag ggaaccgtgg acaaggttcg
     3661 cgagtacgtg atcgcgaacg ggccgcccta catggtgcca aaggagatac tcgtcaggga
     3721 cgagctgccc aagactggct caggcaagat cgaccgcaag gggatcagca atgcttacgc
     3781 aatcggataa gg
//
LOCUS       DBNGVD010000523         6061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3414532, whole genome shotgun sequence.
ACCESSION   DBNGVD010000523 DBNGVD010000000
VERSION     DBNGVD010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3414532"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC16CM_14125"
     CDS             complement(<1..298)
                     /locus_tag="AC16CM_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR3141018.1"
                     /translation="MEAVIFPAHRILAALLLVALNGLFVAAEFSLVKIRATQVDRLVE
                     EGRAAAGLVKEATGKLDAYLAVCQLGITISSLGLGALGEPAIATLIEPLLEPLGI"
     gene            complement(479..1513)
                     /locus_tag="AC16CM_14130"
     CDS             complement(479..1513)
                     /locus_tag="AC16CM_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3141019.1"
                     /translation="MTTVVIASYFEEEYVRRIEEVDERLRVLYKEDLVPPPRWPGDHA
                     GPGEWERSPGDEEEFLGMLGEAEVLYDFPRGHLEDLVRVAPNLRWVQGSMAGAGEMAE
                     KAGLGETEVVVTTASGIYSGPLAEFVLMAMLQHVKGLDDLRRDKAEKVWRQGAVGGLE
                     RKTLCVVGVGSIGQAIATRARAFGMRVVGVKRKVSAEDEAWQYADELYPTAELHEALG
                     EADFVAVTLPGTPQTHHLLDEDAFAAVRRGAYFVNVGRGRVVDEAALVRALENGQLSG
                     AALDVFEVEPLPRESPLWELDNVIISAHTTDVVPDLINSAQTDLFCENLRRYLAGEAL
                     INVLDKRLMY"
     gene            1604..2230
                     /locus_tag="AC16CM_14135"
     CDS             1604..2230
                     /locus_tag="AC16CM_14135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3141020.1"
                     /translation="MDELLDILDETGRPTGEVLEKSEAHRRGLWHRCFHCWICGSDAA
                     GHYLLLQRRAFTKDTWPGYLDITAAGHLSAGERTLDGLREIEEELGLKVDPERLIPLG
                     TISIEQPLPAGCDREFHEVFLLQDDTPPENLRLQKEEVESIYRIDLYDVETLLETGET
                     PAREYRQGDSIATRINLEEFVPKNEDYLAHIAEAARQLLSGTRPPPPF"
     gene            complement(2369..3244)
                     /locus_tag="AC16CM_14140"
     CDS             complement(2369..3244)
                     /locus_tag="AC16CM_14140"
                     /inference="COORDINATES: protein motif:HMM:NF018436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HIR3141021.1"
                     /translation="MAIGERSPVLSRSRPQTEEIEDQAEQAIEQASPWVERLARLGYV
                     AKGVVYAAIGFLALREALGVGGETTDPTGVMHLVSTQPLGGLMLALLAVGLACYALWK
                     VVQGVADPDGKGSDAHGLVRRVGYVGSGIIYSVLSYTAAQSIFGAEDTSEDAMAASVM
                     ALQPPLGPILVFVAGTIVIGVGLYQLYAAYKAKFRDDLRLGQMSAAEERWVVLAGRFG
                     TAARAIAIGVAGAFLLLAVYQSDPSETRGLGAALEIVQHQPLGSFMLGAIAVGLLIYG
                     AFMFAIARYRRIGRT"
     gene            3330..5054
                     /locus_tag="AC16CM_14145"
     CDS             3330..5054
                     /locus_tag="AC16CM_14145"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3141022.1"
                     /translation="MRVALAQINTTVGDIWGNVERMSDALKRAVDSGADLVAFPELTI
                     PGYPPEDLLMRPNFIEENMRALNEFAGQVPEDIVAAVGFVDLDADLYNACAVLSGGEV
                     LHRYHKQYLPNYGVFDENRYFREGSGAPIVDLGGDLVGVSVCEDIWYPGGPAREQALS
                     GASVLLNISASPYHRRKGAFRERMLGVRASDYGCYVVFCNLVGGQDELVFDGHSVVFD
                     PEGSLVARAAQFREDFQIVDLYTDQSLMQRLHDPRPRKENPERPPAVIKVPLHDPAPA
                     ESVEARVEPSLSEEGEVLEALVLGLGDYFRKNGFSRAVLGISGGIDSSLGAVVAAEAL
                     GPENVTGVLMPSRYTSDLSNTDAAALAKNLGIDTQTVPIGPAFDAYEKMLRDAFKGLP
                     EDVTEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDSAGGFAVIRDV
                     PKTMVYKVSNHYNEKKNREIIPDSILDKEPSAELREDQRDTDSLPPYDVLDPILEAYI
                     EEDMGIAQIVDSGFEEEDVRRIVQLVDRAEYKRRQAPTGIKVTGRAFGRDRRMPITNR
                     YFERNDEG"
     gene            complement(5135..>6061)
                     /locus_tag="AC16CM_14150"
     CDS             complement(5135..>6061)
                     /locus_tag="AC16CM_14150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3141023.1"
                     /translation="EMLWALGMILLGLTATVSPEPEASAGTTRIQPWRIVLYWLGPLS
                     PPLQLAVLLIWGALNPPLPAYALAACAVVLLYMAVRVGLVSSVSKQLGQEREESARQG
                     AESRLLYEMHDTVKQNVHGISLTLRSALDADRRGDRERMREMFEKALNAARGAEFQIS
                     RPYDELSAMREETPPGAGDYLRQRLQKFEEYFGIKTHHDLQASLEALNPSEAAALNRI
                     AVEAFWNVAKHSRANNLYLESRQIGSVLIVRIRDDGCGFDSTHPHPGLGLSYMRQRTA
                     EVNADLDVISTPGRGASVQARFRRRGLITQST"
BASE COUNT         1140 a         2075 c         1864 g          982 t
ORIGIN      
        1 tgatcccaag cggctcgagc aagggctcga tgagggtggc gatcgcgggt tcgccgaggg
       61 cgccaaggcc aagcgacgag atggtgatgc cgagctggca gaccgcaaga taagcgtcga
      121 gcttccccgt agcttccttc accagcccgg ccgccgccct accctcctcc actagccggt
      181 cgacctgagt ggcccttatc ttgaccagcg agaactccgc cgcgacgaag agaccattca
      241 aagcgaccag caggagcgcg gcaaggatcc tgtgggcggg aaatattacg gcttccagaa
      301 tgactccttg ggagggttga aagtgcgcgc ccccttacag ggcgcgcaga tactcgatgt
      361 ctcgctagtc tccatgcgac tgaagcaact ataccattcg ggttgcctgg acaaaccgcg
      421 ccccgcgcct acagcaatca gctatcagca aaaacaaaaa agctgatagc tgaccgctct
      481 aatacatcaa ccgcttgtcg agcacgttga tcaaagcttc ccccgccagg tagcggcgca
      541 ggttctcgca gaacaggtcg gtctgcgcgc tgttgatgag gtctggcacc acgtcggtgg
      601 tgtgtgcgct tatgataacg ttgtcgagct cccacaacgg gctctcccgc ggcagaggct
      661 cgacctcgaa cacgtcgagc gcggcccctg atagctgccc gttctccagc gcccttacga
      721 gcgccgcctc gtcgaccacg cgtcccctcc ccacgttgac gaagtaggcg ccccgcctga
      781 cggcggcgaa cgcatcctcg tcgagcaggt ggtgcgtctg tggggtaccc gggagcgtga
      841 cggccacgaa atccgcctcc ccgagcgcct cgtgcagctc agccgtcgga tacagctcgt
      901 ccgcgtactg ccaggcctca tcctcagcgc tcaccttgcg cttgaccccg accacgcgca
      961 tgccgaacgc ccgagcccgc gtggcgatgg cctgaccgat gctgcccacg cccaccacgc
     1021 agagcgtctt gcgctccagg ccgccaacgg ccccctgacg ccagaccttc tccgccttgt
     1081 cccgtctcag gtcatcgagg cccttgacgt gctgcagcat cgccatcagc acgaactctg
     1141 cgagcggccc cgagtagatg ccgctggccg tcgtaaccac gacctcggtc tcgcccagcc
     1201 cggccttctc ggccatctcc ccggccccgg ccatgctccc ctgcacccac ctcaggtttg
     1261 gggcgacccg cacgaggtcc tcgagatgac cccgcggaaa atcgtagagt acctccgcct
     1321 cccccagcat cccgagaaac tcctcctcgt ccccgggcga cctctcccat tcccctggac
     1381 cggcgtgatc cccaggccac cgggggggcg ggacgagatc ctccttgtag agcacccgca
     1441 gcctttcgtc gacctcctcg atgcggcgga cgtactcttc ctcgaagtag ctggcgataa
     1501 cgacggtagt caacgaatct ccttttatgc aggtttcagg cttcaggcta caggtttttc
     1561 tcgctcgtgt ggtgccggtg gactacatgc taccctgtgg cgcgtggacg agttgctcga
     1621 catcctggac gagaccggcc ggccgacggg ggaggtactc gagaagagcg aggcgcaccg
     1681 gcgcggcctc tggcaccggt gttttcactg ctggatctgc ggctccgacg ccgcgggcca
     1741 ctacctgctc ctccagcgcc gcgccttcac caaagacacc tggccgggct acctcgacat
     1801 caccgccgca ggacacctct cagcgggcga gagaacgctc gacggcttgc gggagataga
     1861 agaagaactt ggcctgaagg tagacccgga aaggctgatc cccctcggaa ccataagcat
     1921 agagcaaccc ctaccagctg gctgcgaccg agagttccac gaggtcttcc tcctccagga
     1981 cgacaccccc ccagaaaacc tgcgcctgca aaaagaagag gtcgaatcta tctaccggat
     2041 agacctctac gacgtggaga cgctactcga aacaggtgag accccagccc gcgaatacag
     2101 gcaaggcgat tcaatcgcaa ccagaataaa cctagaagag ttcgtcccca aaaacgaaga
     2161 ctacctggcc catattgccg aagcagcccg ccagctcctc tccggcaccc gcccgccccc
     2221 acccttctag ccgaaagcga ggcgaagccg aagcgttgct gacaagcgac agccgccagg
     2281 ctggagcgtg ctgacaagct ggcgaagcgg agcgaagccg gcgtgctgac aagcggaggc
     2341 cgttaggccg aagcgtgcgg acaggccctc aggtccgtcc aatcctgcgg taacgggcga
     2401 tcgcgaacat gaaggcgccg tagatcagca gcccgacggc gattgcgccg agcatgaacg
     2461 agccgagggg ttggtgctgg acgatctcga gcgcggcgcc gaggcccctg gtctcgctcg
     2521 ggtccgactg gtacacggcc agaagtagga aggccccggc gacaccgatg gcgatggcgc
     2581 gggccgccgt gccgaaccgg ccggcaagga cgacccaccg ttcctccgcc gcgctcatct
     2641 gtcccagccg caagtcgtcc cgaaacttcg ctttgtaggc cgcgtagagc tgatagaggc
     2701 cgaccccgat cactatcgtt ccggcgacaa agaccaggat aggacctaac ggcggttgga
     2761 gggccatgac gctagccgcc atggcgtcct ccgaggtgtc ctcggccccg aagatagact
     2821 gcgccgccgt ataggagagg acgctgtaga taatgccgct ccccacgtat ccgacgcgcc
     2881 gcacgaggcc gtgggcgtcg gaccccttgc cgtccgggtc agccacgccc tgcaccacct
     2941 tccacagggc gtagcaagcg aggccgacgg ccagcagggc gagcatgagt ccccctagcg
     3001 gttgcgtcga cacgaggtgc atgacgccgg tcgggtccgt ggtctccccg ccaacgccta
     3061 gtgcctcgcg cagcgccaga aagccgatgg cggcgtacac cacgcccttc gcgacgtatc
     3121 cgagccgcgc cagccgttcc acccaggggc tggcctgctc gatcgcctgt tcggcctggt
     3181 cttcgatctc ttccgtctgc ggccggctcc gtgacaggac cggactcctt tctccgatgg
     3241 ccacccggta ctctacaccc ctgatcgggt ccaggctccc cataccgcat cggcgggcaa
     3301 gaacgtgcca ggcacgatat atttagctta tgagggtagc gctggcccag atcaacacca
     3361 ccgtcgggga catctgggga aacgtcgaga ggatgtccga cgccctgaag cgggccgtcg
     3421 actcgggcgc cgacctcgtc gccttccccg agctgacgat ccccggatac cctcccgagg
     3481 acctcctgat gcgcccgaac tttatagaag agaacatgcg ggccctgaac gagttcgccg
     3541 ggcaggtccc ggaggacata gtcgccgccg tcggcttcgt cgacctcgac gccgacctct
     3601 acaacgcctg cgccgtactc tcgggcggcg aggtcctgca ccgctaccac aagcaatacc
     3661 ttcccaacta cggcgtcttc gacgagaatc gctacttcag ggagggctcg ggggccccga
     3721 tcgtggacct cggtggcgac ctcgtcgggg tgagcgtgtg cgaggacatc tggtaccctg
     3781 gcgggccggc gcgcgagcag gctctctccg gggcaagcgt gctcctcaac atctcggcct
     3841 ccccctacca ccggcgcaag ggcgctttca gggagcggat gctgggcgtg cgggcctcgg
     3901 attacggttg ctacgtggtg ttctgcaacc tcgtcggcgg ccaggacgag ctcgtcttcg
     3961 acgggcattc tgtggtcttc gaccccgagg gctcgctcgt ggcgagggcc gcccagttca
     4021 gggaagactt ccagatcgtg gacctctaca cggaccagtc gctcatgcag cgcctccacg
     4081 accccaggcc gcgcaaggag aaccccgaac gcccccccgc ggtcataaag gttcccctcc
     4141 acgacccggc ccccgctgag tctgtcgagg cgcgggtaga gccgtccctc tcggaagagg
     4201 gagaggttct cgaggcgctt gtgctcggcc tcggggacta cttccgcaag aacggcttct
     4261 cccgggcggt gctaggtatc tcgggtggta tcgactcctc cctgggggcc gtcgtggccg
     4321 ccgaggccct cggccccgag aacgtgaccg gcgtcctcat gccgagccgc tacacctcgg
     4381 acctgagcaa caccgacgcc gccgcgctcg ccaagaacct gggcatagac acccagaccg
     4441 tgccaatagg ccccgccttc gacgcctacg agaagatgct gcgagacgcc ttcaagggcc
     4501 tccctgaaga cgtcacggag gagaacctgc agagccgcat caggggcaac atcctcatgg
     4561 ccctctccaa caagtttggt tggatcgtcc tctccaccgg caacaagagc gagatgagcg
     4621 tgggatactc caccctctac ggcgactccg ccggcggctt cgcggtcata agggacgtgc
     4681 ccaagaccat ggtctacaag gtgtccaacc actacaacga aaagaagaac cgcgagatca
     4741 ttcccgactc catcctcgac aaggaaccga gcgccgagct acgcgaagac cagcgcgaca
     4801 cggactccct acccccctac gacgtgctcg accccatcct cgaagcctac atcgaagaag
     4861 acatggggat agcccagatc gtcgactccg gcttcgaaga ggaagacgtc agacgaatag
     4921 tccaactggt tgacagggcg gaatacaaac gccgccaggc ccccacgggc ataaaggtaa
     4981 caggccgcgc cttcggccgg gaccgccgca tgcctataac gaaccgctat ttcgagcgta
     5041 acgacgaagg ataggacagg gccgtcaccc gtaggtgcgc tgtaggaacg cttcgcaaag
     5101 ctcccgtacc gctcctcggc tctctagaga tgttctatgt gctctgggtg atgaggccgc
     5161 gcctccggaa ccttgcctgc accgaggcgc cgcggccagg ggtggagatc acgtccaggt
     5221 cggcgtttac ctcggccgtg cgctggcgca tgtaggacag gcccagacca ggatgcgggt
     5281 gggtggaatc gaagccgcag ccgtcgtccc tgatgcggac gatcagcacc gaccctatct
     5341 ggcgggactc caggtacagg ttgttggcgc gggagtgctt ggccacgttc cagaaggcct
     5401 cgacggcgat cctgttcagg gcggcggcct ctgacgggtt cagcgcttcg agggaggcct
     5461 ggaggtcatg gtgggtcttg atgccgaagt attcctcgaa cttctgcagg cgctggcgga
     5521 ggtaatcccc ggcccctgga ggggtctcct cgcgcatggc ggagagctcg tcgtaggggc
     5581 gtgagatctg gaactccgcc cccctggccg cgttcagggc cttctcgaac atctccctca
     5641 tcctttccct atccccgcgg cggtcggcgt cgagggcgga gcggagcgtc agcgagatgc
     5701 cgtgcacgtt ctgcttgacc gtgtcgtgca tctcgtagag gagacggctc tcggcgccct
     5761 gacgggcgga ttcctccctc tcctggccaa gctgcttgct caccgaggag accagcccga
     5821 cgcgtacggc catgtagagg agcacaacgg cgcaggcggc gagcgcgtag gcgggcagcg
     5881 gcgggttcag tgcgccccag atcagcagaa cggctagctg caggggcggg gagagcgggc
     5941 ccagccagta caggactatg cgccagggct gtatccgggt cgtgcccgcg gaagcctcgg
     6001 gctcagggga gaccgtcgcg gtgaggccga gcaggatcat gccgagggcc cacagcatct
     6061 c
//
LOCUS       DBNGVD010000524         5201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1957819, whole genome shotgun sequence.
ACCESSION   DBNGVD010000524 DBNGVD010000000
VERSION     DBNGVD010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1957819"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="AC16CM_14155"
     CDS             <1..979
                     /locus_tag="AC16CM_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3141024.1"
                     /translation="PEGMNALVTRLMAKNPEDRYASADELADDLWKITRGREPTAATD
                     AAAAPTRVQRGPAAAAYTPTQRVPTVHTSPVRRRRRRVSWFMVLLVALLLLLGTLGVL
                     RGLLGPDFANGWFESPGENNPQSGPSASQTVKVPRVAGLTIAEARQRLTDAGLGVGQI
                     NSLPSDNVAAGRVIASGYSAGTKLPRDTEVNLDVSTGPQAAPSPSPTATAQPSPQHAG
                     SASASATAQPSQQQNRQPARQPGRRQNAAPVRPDDNAGASPSPSAAPEDNAAPQGAGG
                     LEDNSGSGISGNGNSGNGTSGSGISGNGNSGNGNGGGGNSGPGGGRGQP"
     gene            1252..2223
                     /locus_tag="AC16CM_14160"
     CDS             1252..2223
                     /locus_tag="AC16CM_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3141025.1"
                     /translation="MKAVRIHDFGGPEELSYEDLEMPEPGPGEARVKLAASGVNFIDV
                     YQRTGLYPGDLPRPLGLEGAGEVDAVGEGVEEVSPGDYVAFASAPGSYAEYVVAPVEA
                     LVPFNVTLVEARVAAAVMLQGMTAHYLTHSTFPLQEGQTALVHAAAGGVGLLLVQMAK
                     LRGATVIGTAGTEEKAQLARGAGADEVILYTEQDFVEETNRITDGEGVHVVYDSVGKE
                     TFDGGLDVLRPRGYMVLFGASSGPVEPVDPQILNQKGGLFLTRPALWQYTADREELLW
                     RAESLFSWIGQNNLDVRIGGTYELSDVEQAHRDLEGRKTTGKLLLIP"
     gene            2233..3258
                     /locus_tag="AC16CM_14165"
     CDS             2233..3258
                     /locus_tag="AC16CM_14165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3141026.1"
                     /translation="MKLDAALGTEGNPLKGMGRTARAAEELGFAGLWTSETKHDGFLP
                     LAVAASETERMELGTSVAIAFSRSPMETAQTAWDLQDLSRGRFLLGLGTQVKAHITRR
                     FSMPWDRPAARLREYILALREIWRSFQDEGSPDFEGEFYSHTLMTPFFNPGPIEHPEI
                     PVYIAGVNTSLARLAGEACDGFHVHPFHSPEYVRQTVKPAISEGADRAGRDPGRVELV
                     TSAFVVPTGNGEEAAEQRESVRAQISFYASTPTYRTVLEAHGWEQVGERLGTMAREKK
                     WREMPALITDEMLAAFAIEAAPDEIGPALKERYEGLIDRVALYLSFVPGERDEFWQTV
                     VDSIRSA"
     gene            complement(3332..4501)
                     /locus_tag="AC16CM_14170"
     CDS             complement(3332..4501)
                     /locus_tag="AC16CM_14170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3141027.1"
                     /translation="MKAVVWHGKEDVRVDDVPDPIIEQPTDAIIRITSTAICGSDLHL
                     YAKLSPLMREGDIIGHEPMGIVEEVGADVKHIAPGDRVVVPFNVSCGHCFFCDQDLYS
                     QCETTRDTGKLAKSVNLLGRGRGASLLGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPE
                     GPPDERFLYLSDVLPTSWQAVAYADVPEGGTVGIWGLGPVGQMCARIAMQQGAGRVIG
                     IDNVPERLALASRWGVQTIDYSAVDGVRDVILDLTDGRGVDSAIDAVGMEAGGSLVGS
                     ILQATKLQLDRATALRESMSAVRRGGTLSLSGVYAGPIQAFPLGDLFDMQVQVRMGQA
                     NVRQWVDDIMPLLTDEDPLGVEDLKTHEMPLEDAPLGYEIFQKKQDGAIKVVLKP"
     gene            complement(4498..>5201)
                     /locus_tag="AC16CM_14175"
     CDS             complement(4498..>5201)
                     /locus_tag="AC16CM_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3141028.1"
                     /translation="KDSRVDESWPTGGIPTSYYGRHKAEVERRLDRFEREAPEVRVVR
                     MRPGLVFKREAAEGIRRLFAGPFFPSALADPALINLVPDIQDLRSQVVHSYDVGEAFR
                     LAIVNEGAGGAFNLATEPVLDAEEIGRILNARTVPVSSRLARAGIGLSWRFRLQPVPP
                     GWLDLARQVPIMDTTRAGTELGWTPQFSADETLLDVMGGLKAASGLDTPPLSPQTGGP
                     LRIREILTGVGKREP"
BASE COUNT          909 a         1766 c         1738 g          788 t
ORIGIN      
        1 acccgagggc atgaacgccc tggtgaccag gctcatggcc aagaacccgg aagaccgcta
       61 cgcctccgcc gacgaactcg cagacgacct ctggaagata accagggggc gcgagcccac
      121 cgcggcgacg gacgccgcgg cggctccgac ccgggtgcag cgggggcccg ctgccgccgc
      181 ctacaccccc acacagagag tcccgacggt ccatacatcg ccggtcaggc gtcgccgcag
      241 gcgtgtctcc tggttcatgg tgctgcttgt cgcgcttctg ttactgctcg gtaccctggg
      301 cgttctcagg ggcctgctgg gcccggactt cgcgaacggc tggttcgaat cgcccgggga
      361 gaacaacccg cagtccgggc cctccgcgtc ccagaccgta aaggttcccc gggtcgcggg
      421 gctcaccatc gccgaggcac gtcagaggct aaccgacgcc gggctcgggg tcgggcagat
      481 caactccctc ccgagcgaca acgtggccgc cggcagggtc atcgcctccg gctacagcgc
      541 gggcacgaag ttgccgcgcg acaccgaggt gaacctggac gtgagcaccg gcccgcaggc
      601 agccccgtcg ccttccccga ccgcgacggc ccagccgagc ccacaacacg caggctcggc
      661 cagcgcctcc gcaacggccc aaccctccca acagcagaac cgccaacctg ccaggcaacc
      721 cggtcgccgg caaaatgccg ccccggtccg accggatgac aacgccggag ccagcccctc
      781 gccaagcgca gccccagaag acaacgcggc gccacagggc gccggtggac tcgaagacaa
      841 cagcggcagc gggatcagcg gtaatgggaa cagcggtaat gggaccagcg gcagcgggat
      901 cagcggtaat gggaacagcg gtaatgggaa cggcggcggc gggaacagcg gccccggtgg
      961 tgggagaggc caaccctagg caaacccatc ttctggcatc gttggcccgg acggcccgag
     1021 catcccctct gatactccct gaggtcccgc gttcctggta aagatagtgc ggcgaacgcg
     1081 acggtccgtt cgcggcggga gagcaggatc gtcggggtgg atccggtgag gcgcccagca
     1141 cgccggcaag cagcgaccac gggccgaagc gtgcaggcgg ggcgcgtgga gccgggccaa
     1201 caagctacaa taaccgacgc gtatgccgag cttacagggg aggagtgcac catgaaggcc
     1261 gtacggatac acgactttgg tgggccggag gagctgagct acgaggacct ggagatgccc
     1321 gagccgggtc ccggtgaggc acgcgtgaag ctcgctgcct ctggcgtgaa ctttatcgac
     1381 gtctaccagc gcacgggcct ctaccccggc gatctgccgc ggccgctcgg cctggagggt
     1441 gcgggcgagg tggatgccgt gggagaaggt gtcgaagagg tatcccccgg agattacgtg
     1501 gccttcgcga gcgcgccggg ctcctacgca gagtacgtgg tcgcccccgt ggaggccctc
     1561 gtgcccttca acgtcacgct cgtggaggcg cgggtggccg ccgcggttat gctccagggc
     1621 atgacggcgc actacctgac ccacagcacc ttccccctgc aggaggggca gacggcgctc
     1681 gttcatgccg cagccggcgg ggtcgggctg ctgctcgtcc agatggccaa gctgcggggg
     1741 gccacggtga tcgggaccgc cggaaccgag gagaaggcgc agctcgccag gggggctgga
     1801 gccgacgagg tgatcctcta cacggagcag gacttcgtag aggagacgaa ccgcatcacg
     1861 gacggcgagg gcgtgcacgt ggtctacgac tccgtgggca aggagacctt cgacggcggc
     1921 ctcgacgtgc tgcgcccgcg cggctacatg gtgctattcg gcgcgtccag cggtcccgtg
     1981 gagccggtcg acccccagat cctcaaccag aagggcgggc tgttcttgac acgacctgcg
     2041 ctctggcagt acacggcaga ccgcgaggag ctcctgtggc gcgccgagag cctcttctcc
     2101 tggatcggcc agaacaacct ggacgtccgg attggcggca cctacgagct ctccgacgtg
     2161 gagcaggccc accgcgacct cgaaggccgc aagacaacgg gcaaactgct gctcatcccg
     2221 taagacggac ccgtgaagct cgacgccgcc ctcggaacgg aaggcaaccc cctcaaaggg
     2281 atgggacgga ccgcccgcgc cgccgaagag ctcggtttcg ccggcctgtg gaccagcgag
     2341 acaaagcacg acggtttcct gccgctggca gtcgccgcca gcgagacgga acggatggag
     2401 ctcggcacct ccgtggccat agccttctcg cgctcgccca tggagacggc ccagacggcg
     2461 tgggatctgc aggacctctc caggggccgc ttcctgctag ggctcggcac gcaggtcaag
     2521 gcccacataa cccgccgttt ctccatgccg tgggacaggc cggcggcacg cctgcgcgag
     2581 tacatcctgg ccttgcgtga gatctggcgc tccttccagg acgagggctc cccggatttc
     2641 gagggtgagt tctacagcca caccctcatg acgcccttct tcaaccccgg acccatagaa
     2701 catccggaga tcccagtcta catcgccggg gtcaacacca gccttgccag gctggcgggc
     2761 gaggcctgcg acggcttcca cgttcacccc ttccactcgc cggagtacgt gcgccagacc
     2821 gtcaaaccgg ccatctcaga gggcgcggac agggccggcc gtgaccccgg gagagtcgag
     2881 ctggtgacga gcgccttcgt cgtccccacg ggaaacgggg aggaggccgc agagcagcgc
     2941 gagtcggtga gagcccagat ctctttctac gcctccaccc cgacctaccg caccgtcctc
     3001 gaagcgcacg gctgggaaca ggtcggcgaa aggctgggga ccatggcccg cgagaagaag
     3061 tggcgtgaga tgcccgccct cataaccgac gagatgctcg ccgccttcgc tatagaggcc
     3121 gccccagacg agattggccc ggccctgaaa gagcgctacg agggcctcat agaccgcgtc
     3181 gcgctctacc tatcgttcgt cccaggagaa cgggacgagt tctggcaaac ggtggtggat
     3241 tcgatccgaa gtgcatgagg ctcttgcctc atgcacttca gctgtcagct ttcagcgatc
     3301 agcggtcagc aagtgaagct gtcagctaca tttacggctt caggaccacc tttatggcac
     3361 catcctgctt tttctgaaag atctcgtatc caagcggggc gtcttcgagg ggcatctcgt
     3421 gggtcttcag gtcctcgacg ccgagtgggt cctcgtcggt cagcagcggc atgatgtcat
     3481 cgacccactg cctcacgttg gcctggccca tacgaacctg gacctgcata tcgaagaggt
     3541 cgccaagggg gaaggcctgg atgggacctg cgtagacgcc tgagagcgag agcgtccccc
     3601 cgcgcctgac ggccgacatg ctctcgcgca gggccgtggc ccggtccagt tgaagcttgg
     3661 tggcctgcag gatggagccc acgagcgacc cgccggcctc catgccaacg gcatctatcg
     3721 cggagtccac gccgcgtccg tcggtgaggt cgaggatcac gtcccgcacg ccgtcgacgg
     3781 ctgagtagtc tatggtctgc acgccccatc tggaggcgag cgccagccgc tccggcacgt
     3841 tgtctatgcc aatgacgcgc ccggcaccct gttgcatcgc gatgcgggcg cacatctggc
     3901 cgacgggacc gaggccccag atccccaccg tcccgccctc gggaacgtcg gcgtaggcca
     3961 cggcctgcca ggaggtgggc agcacgtcgg agaggtacag gaagcgctcg tccggcgggc
     4021 cttcggggac cttgatcggc ccgaagtggg cctggggcac ccggaggtac tcggcctgtc
     4081 cccccgggac ggcgccgtag acgtgcgtgt agcccagcag gctcgcgccc ctaccccgcc
     4141 cgaggaggtt cacgctcttg gctagtttcc ccgtgtccct ggtcgtctcg cactgggagt
     4201 acaggtcctg atcgcagaag aagcagtgac cacaggagac gttgaagggg acgacgacgc
     4261 ggtcgcctgg cgctatgtgc ttgacgtcgg cccccacctc ttcgacgatg cccatgggct
     4321 cgtggccgat gatgtcgccc tcgcgcatca gcggcgagag cttggcgtag aggtgcaggt
     4381 cggagccaca gatggcggtg gaggttatgc ggatgatagc gtcggttggc tgctcgatga
     4441 tcgggtcggg aacgtcgtcc accctgacgt cctccttgcc gtgccagact acggccttca
     4501 aggctccctc tttcctactc cggtgagtat ctcgcgtatc cgtaatggcc ccccggtctg
     4561 cggggagagc ggcggcgtat cgagcccgct cgcggccttc aggccgccca tgacatcgag
     4621 cagcgtctcg tcggcggaga actgcggcgt ccagcccagc tccgttccgg cccttgtggt
     4681 gtccatgatc ggaacctgtc gggcgaggtc cagccatccc ggtgggaccg gctgcagcct
     4741 gaacctccac gagagaccga tgccggcgcg cgcaagccgc gaggagaccg gtacggtacg
     4801 ggcgttcagg atgcgaccga tctcttccgc gtccagcacg ggctcggtcg ccaggttgaa
     4861 agcgcccccg gctccctcgt tcacgatggc gagccggaag gcctcgccca cgtcgtagga
     4921 gtggaccacc tgagagcgca ggtcctggat atcagggacg aggttgatca gggccgggtc
     4981 ggcgagcgcg ctcgggaaga agggcccggc gaagaggcgc ctgatcccct ccgccgcctc
     5041 acgcttgaac acgagccctg gcctcatcct gactaccctt acctcgggcg cctcccgctc
     5101 gaagcggtcg agcctccgct ctacctcggc cttgtgtcgc ccgtagtacg aggtcgggat
     5161 gcccccggtc ggccagctct cgtccacacg actatctttc g
//
LOCUS       DBNGVD010000525         2562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4144631, whole genome shotgun sequence.
ACCESSION   DBNGVD010000525 DBNGVD010000000
VERSION     DBNGVD010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4144631"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..715
                     /locus_tag="AC16CM_14180"
     CDS             20..715
                     /locus_tag="AC16CM_14180"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HIR3141029.1"
                     /translation="MNTPSVVSPEEWKTAREDLLVKEKEHTRARDALAAERRRMPWMA
                     VEKEYGFEGPNGPATFADLFEGRRQLIVYRAFYGPEVTTLANPERGDAYPERACAGCS
                     FLADQVAYPAHLNARDVTLVFASRAPQTDIQKLKERHGWEHIPWYTITSDWDRDFDVD
                     EWHGHNAFIREGEKIYRTYFIDGRGDEAMGTTWSYLDMAPLGRQEDWESSPEGYPQTA
                     PYGWWDYKDSYGT"
     gene            951..1286
                     /locus_tag="AC16CM_14185"
     CDS             951..1286
                     /locus_tag="AC16CM_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141030.1"
                     /translation="MSEASGRQEMERRLVERSLEDGDFRRSLLADPRTTTERELGVRL
                     PEEVRVVAVEETADTIYLVLPGVSATNEEISDQELETVAGAGDTFMCTYTCPPPEGAT
                     CATCGGCTG"
     gene            1485..2297
                     /locus_tag="AC16CM_14190"
     CDS             1485..2297
                     /locus_tag="AC16CM_14190"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3141031.1"
                     /translation="MFPWEISAAMAEAPLCYLPLGTLEWHGEHAAVGLDALKAHAICV
                     GAARRSGGLVLPPIYWSADWREDLPDGEYLTGGIERGERYHVPGNMFWVRPDTFHRLL
                     LDAYEAMHRRGFRAIVVLSGHWSIEHNIAAIRRTGEEFRRSHPATGWLLLTDQEVVPD
                     LHYPVEHAAGGETSLLMAIRPDLVALDKMFETDRSLRHYYAGEEKHLQRRRETPHKYI
                     GVNPAVADASNDPEGATVERGRMLLEAIVERVAVRARTLLETTRGAKDPPPN"
     gene            complement(2316..>2562)
                     /locus_tag="AC16CM_14195"
     CDS             complement(2316..>2562)
                     /locus_tag="AC16CM_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3141032.1"
                     /translation="TTILSFAAIFAAVSVRTGALTVVPLIVGVFLGSASWWLLLSTGA
                     SLIRDRVGPRGMRAVNIVSGAAILTFGLAAVGAGILG"
BASE COUNT          495 a          822 c          860 g          385 t
ORIGIN      
        1 aggaagagga aggagcgaag tgaacacacc atccgtcgtt tctcccgagg agtggaaaac
       61 tgcccgcgag gatttgctcg tcaaggagaa ggagcacacc cgagcccgtg acgcgcttgc
      121 ggcagagcgc cgcaggatgc cgtggatggc ggtcgagaag gagtacggct tcgaaggccc
      181 gaacgggccg gctacgttcg ccgacctgtt cgagggccgc cgtcagctga tcgtctaccg
      241 tgccttctac gggccggaag tcaccacact ggccaatccg gagcgcggcg acgcctatcc
      301 cgagcgggcg tgcgcgggct gttcctttct ggctgaccag gtcgcctatc cagcccacct
      361 caacgcgcgg gacgtgacgc tggtgttcgc ctcgcgcgca ccgcagaccg atatccagaa
      421 gttgaaggag cgtcacggtt gggagcacat cccctggtac acgatcacca gcgactggga
      481 cagggacttc gatgtcgacg agtggcacgg ccacaacgcg ttcatccgcg agggtgagaa
      541 gatctaccgc acctatttca tcgacggccg cggcgatgag gcaatgggca cgacctggag
      601 ctacctcgac atggccccgc tcggccgcca ggaagattgg gagagctctc ccgagggcta
      661 cccgcaaacg gcgccctacg ggtggtggga ctacaaagac tcctacggca catgagcgcg
      721 tagccgcgca gtcgatatag agatgttcgc atccagcctc cggtgcgaaa ggcgcgggag
      781 gtgatctgat acaggggaac ccggaaggaa ctcgtttccc tcccggacga tgacgcgggc
      841 cgctcttccc tttgcctggc ggcgggtggc gtgggatact gccacctccc gcctcgcttc
      901 caggggcgac acgaaagtgg agcggaaaga acgaaagggg agaggagcgc atgagcgaag
      961 cgtctggtcg tcaggagatg gagcgcaggc tggtcgagag gagcctcgag gacggcgact
     1021 tccgccgaag cctactcgcc gaccctagga cgaccacgga gcgcgaactg ggtgtccggc
     1081 tgccagaaga ggtgagggta gtggccgtcg aagagaccgc cgacaccatc tacctcgtgc
     1141 ttccaggcgt ctcggccacg aacgaggaaa tctccgacca ggaacttgaa accgtcgccg
     1201 gggccggcga cacgttcatg tgcacctaca cctgtccgcc tcccgaaggt gccacctgtg
     1261 ccacctgcgg cggctgtacc gggtaaccct ccagcgcctc gccaggggcc tcccggatca
     1321 tgaagcacgc tccgtcggac gtcgagggtg cgggctgacc aggttcgcag ggcggccacg
     1381 tgcgggcgcg cagggatgtg gcccgggacc cgagggtatg gcatggtggc attgctatgg
     1441 ccagcggaga aatcagcgat cggcgagagg taagattcga agagatgttc ccctgggaga
     1501 tttcggccgc catggccgaa gctccgctat gctacctccc gctggggacg ctggagtggc
     1561 acggagaaca cgccgccgtc ggactggacg ccctcaaggc gcacgccatc tgtgtagggg
     1621 cggcccggcg ctcgggtggg ctcgtcttgc cgccgatcta ctggtccgcc gactggcgcg
     1681 aggacctgcc agacggggag taccttaccg gcggcatcga gcgcggcgag cgctaccatg
     1741 taccaggcaa catgttctgg gtgcgtcccg ataccttcca ccgacttctg ctcgatgcct
     1801 acgaggcgat gcatcgccgc gggttccggg cgatcgtcgt gctaagcggc cactggtcca
     1861 tcgagcacaa catcgcggcg atcagacgca ccggggagga gttccggcgg tcgcacccgg
     1921 cgacgggttg gctcctcctg acggaccagg aggtggttcc agatctgcac tatccggtag
     1981 agcacgcggc cggaggagaa acgtcgctgc ttatggcgat acggcccgac ctcgtcgccc
     2041 tcgacaagat gttcgagacg gaccgctcgt tacgccacta ctacgccggc gaggagaagc
     2101 atctgcagcg gcgcagggag accccacaca aatacatcgg cgtcaacccg gcggtggccg
     2161 acgcctccaa cgacccggaa ggggccacgg tcgagcgtgg ccggatgctg ctggaggcaa
     2221 tcgtcgagcg cgtggcggtg cgggcacgca ccctcctgga aaccactagg ggggcgaagg
     2281 atcctccccc taattgaccg ctgttttgcc acctcctatc ccaggatccc ggcccccaca
     2341 gcggccaggc cgaaagtaag gatcgcggcc cccgagacga tgtttaccgc cctcatgccg
     2401 cgcggtccaa ccctgtccct gatgaggctc gcccccgtgc tcaagagcaa ccaccacgag
     2461 gccgaaccga ggaacacgcc gacgatcaac ggcaccacgg tcagggcccc cgtcctgacg
     2521 ctcacggcgg cgaagatcgc ggcgaaggac aggatggtcg tg
//
LOCUS       DBNGVD010000526         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1412151, whole genome shotgun sequence.
ACCESSION   DBNGVD010000526 DBNGVD010000000
VERSION     DBNGVD010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1412151"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16CM_14200"
     CDS             <1..290
                     /locus_tag="AC16CM_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141033.1"
                     /translation="YLPEREYTGISEGKAPRLEGIRPLQRTYVFERAGMPPASQTDVG
                     WVPPEVKKRLKEEAKARKAAEAEAKAAQEKASGNGSADGSAEGSANGKENG"
     gene            290..781
                     /locus_tag="AC16CM_14205"
     CDS             290..781
                     /locus_tag="AC16CM_14205"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3141034.1"
                     /translation="MAVSLPEGDLQTRLQRYLDGLENRHGLEESEIVDGVVQVVASPG
                     DIPAVLGTTRETLGLPHLSFITVVDRQGSFELTYELIDFRGGTVRVRTTIEGDEPVIG
                     TVTNIYPTANWHEREAYDMFGVAFAGHPDLRRTYMWQDHFDHHPLLKSFEISTKRTFE
                     GLR"
     gene            786..1973
                     /locus_tag="AC16CM_14210"
     CDS             786..1973
                     /locus_tag="AC16CM_14210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3141035.1"
                     /translation="MLSEERRAHHEGALTEPASSRLVSPDIRDEFGLETIDMNMGPQH
                     PAMHGLLRLILELDGETVVRCDPVMGYLHRCQEKIAENRMYPAVIPLTDRLDYFANMH
                     NEHGYVLAVEDLLDVEIPPRAEYIRVLMCELMRLASHIPSIGFLMLELGAFTPILYAF
                     RERERIQSIFEAVTGARMMFHYIRIGGVKADLPPEIPQKIWDYLESLEDHFQRDFVDL
                     IAGNEIFISRTRNVGKMTQEQAVEMGVTGPALRCTGVDFDIRRDYPYSIYPELEFDVI
                     TDTAGDVEASYRVRIAECLQSIRLIKQCLEKMPEGEVMGDLGRRLRPDEGHGFGRVES
                     PRGEYAVHVVSDGSDTPYRVHYRDPSFCNMQLLQEIVPGHYLPDIMPIMGLVDPVSGG
                     WDK"
     gene            1974..2975
                     /gene="nuoH"
                     /locus_tag="AC16CM_14215"
     CDS             1974..2975
                     /gene="nuoH"
                     /locus_tag="AC16CM_14215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3141036.1"
                     /translation="MQTVLNLLEVEPIRLLISFGAVLFLVLNLAAVLTMAERKVSGYI
                     HLRFGPNRVGPRGLLQPAADVFKLFTKENVSPGRSDIWVFLGAPIAMFLPAALVWLVV
                     PFAPNAVVANLNVAILFFVAITSIGALGVVMAGYGSRSAFSLLGALRGAAQMISYEVP
                     LILSLLGVVMLSGSLSLVDVVEAQGNTIWTWYFLPQLPMFITFYIAGLAEVKRVPFDL
                     PEGESEIVGGFMVEYSGMTWALIQAAEFASMAVMSAVTVTLFVGGWQPPLPFLDLGNF
                     NWVWFGIKTALIMFTFQWIRWSVPRLRMDQLMDLGWKVLVPITLIWLFVTAGAILIF"
     gene            2985..3494
                     /locus_tag="AC16CM_14220"
     CDS             2985..3494
                     /locus_tag="AC16CM_14220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR3141037.1"
                     /translation="MPQVGQGIMKGMGVTLKHLFGKKITRQYPEYKRELPERSRGMLT
                     VDMDRCIACLQCMRICPDHCISIEQTKRDADGSGKPKPFAVGFVIDDSRCMYCSLCVE
                     VCPVNCIYHTEEFEAQAYNRLELARQFGELPVDPRVDPKPTVKKKKKPTGKKAPNTVE
                     KPRTGGEAA"
BASE COUNT          663 a         1100 c         1160 g          632 t
ORIGIN      
        1 agtacctgcc cgagagggag tacaccggga tcagcgaggg caaggccccc cgcctcgagg
       61 gtatacgtcc actccagcgc acctacgtgt tcgagcgggc cggtatgccc ccggcctccc
      121 agaccgacgt cggctgggtc ccccccgagg tcaagaagcg gctcaaggaa gaggccaagg
      181 ccaggaaggc cgctgaggcc gaagccaagg ccgcgcaaga gaaagcctcg ggcaacggca
      241 gcgccgacgg cagtgccgaa ggcagcgcca acggtaagga gaacggttag tggccgtctc
      301 gctgccggag ggcgatctcc agactcggct acagcgctac ctcgacggcc tcgagaacag
      361 gcacgggctc gaggagtcag agatcgtgga cggtgtggtg caggtcgtgg ccagccccgg
      421 cgacataccg gccgtgctcg ggacgacgcg tgagacgctc gggcttccgc acctctcttt
      481 tattacggtc gtggacaggc agggctcgtt cgagttgacc tacgagctga tagattttcg
      541 cggggggacg gtcagggtca ggacgaccat cgagggtgat gagccggtga tcgggacggt
      601 caccaacatc tacccgacag ccaactggca cgagcgtgag gcctacgaca tgttcggggt
      661 ggcgttcgcg gggcatcccg atctgcggag aacgtacatg tggcaggacc atttcgacca
      721 ccacccgctg ctcaagagct tcgagatcag caccaagcgc accttcgagg gattgaggta
      781 gggttatgct aagcgaggaa cgtcgcgcac accacgaggg cgcgctcacc gagccggcga
      841 gcagccgcct ggtgagcccg gatattaggg acgagttcgg gctcgagacc atcgacatga
      901 acatggggcc gcagcacccc gccatgcacg ggctcctcag gctcatcctg gagctcgacg
      961 gggagaccgt agtcagatgc gaccccgtga tgggatatct gcaccgctgc caggagaaga
     1021 tcgccgagaa ccgcatgtac ccggccgtca tccccctgac cgacaggctc gactacttcg
     1081 ccaacatgca caacgagcac ggctacgtgc tcgccgtgga ggacctgctc gacgtggaga
     1141 taccgccgag ggcggagtac atccgcgtgc tcatgtgcga gctgatgcgg ctcgcgagcc
     1201 acataccctc tatcgggttc ctcatgctag agctcggggc gttcaccccc atcctctacg
     1261 ccttcaggga gcgggagagg atacagtcca tcttcgaggc cgtcacaggc gccaggatga
     1321 tgtttcacta catcaggatc ggcggcgtca aggccgacct cccgccggag ataccccaga
     1381 agatctggga ctacctggag agcctagagg accatttcca gcgcgacttc gtcgacctga
     1441 tagcgggaaa cgagatcttc atctcccgca cccgcaacgt tggcaagatg acgcaggagc
     1501 aggccgtgga gatgggtgtg accggaccgg cgctgcgttg cacgggcgtg gatttcgaca
     1561 tcaggcgcga ctacccgtac agcatctacc cggagctgga gttcgacgtt atcaccgaca
     1621 ccgccgggga cgtcgaggcc tcctaccggg tgcgcatcgc ggagtgcctg cagagcatca
     1681 ggctgatcaa gcagtgcctg gagaagatgc cagagggcga ggtcatgggc gacctcggcc
     1741 gcaggctgcg gcccgatgaa ggacacggct tcgggcgggt ggagagccct cgtggtgagt
     1801 acgccgtaca cgtcgtctcg gacggctccg atacgcccta cagggttcac taccgcgacc
     1861 cgagcttctg caacatgcag ctgttgcagg agatcgttcc aggacattac ctgccagaca
     1921 tcatgccgat catgggcctc gtcgaccccg tctccggtgg gtgggacaag tagatgcaga
     1981 ccgtcctcaa cctgctcgag gtcgagccga taaggctact catctccttc ggggccgtgc
     2041 tgttcctcgt gctcaacctg gccgccgtgc tgacgatggc cgagaggaaa gtctcggggt
     2101 acatccacct ccgcttcggg ccgaacaggg tcgggccgcg gggccttctg cagccggccg
     2161 cagacgtctt caagctcttt accaaggaga acgtctcgcc gggcaggtcg gatatctggg
     2221 tctttctcgg cgcgccgata gccatgttcc tgcccgccgc gctggtgtgg ctggtggtgc
     2281 ccttcgcgcc caacgccgtg gtcgccaacc tcaacgtggc catcctgttc ttcgtggcca
     2341 tcacctccat cggagctctc ggcgtggtca tggccggcta cggcagccgc tcggcgttct
     2401 cgttgctcgg ggcgttgcgc ggggcggcgc agatgatctc ctacgaggta ccgctgatcc
     2461 tgagcctcct cggcgtggtg atgctctcgg gtagcctctc gctcgtcgac gtggtcgagg
     2521 cgcaggggaa caccatctgg acctggtact tcctgccgca gcttcccatg ttcatcacgt
     2581 tctacatagc agggctcgcc gaggtaaagc gggttccctt cgacttgcct gaaggggaga
     2641 gcgagatcgt cggcgggttc atggtcgagt actcggggat gacctgggcg ctcatacagg
     2701 cagccgaatt cgcctccatg gccgtcatgt cggcggtcac cgtcacgctg ttcgtcggcg
     2761 gctggcagcc gcccttgccg ttcctcgatc tcgggaactt caactgggtc tggttcggca
     2821 tcaagaccgc tttgataatg tttaccttcc agtggataag gtggagcgtg ccccgtctca
     2881 ggatggacca gctcatggac cttggctgga aggttctggt tccgatcacc ctgatctggc
     2941 tgttcgtcac ggccggcgcg attctgattt tctaggaggt tgtgatgccg caggttggac
     3001 agggcattat gaaggggatg ggggtgaccc tgaagcacct cttcgggaag aagatcaccc
     3061 gccagtaccc ggagtacaag cgcgagctgc cggagcgcag ccgcggcatg ctcaccgtgg
     3121 acatggaccg ctgcatcgcg tgtctgcagt gcatgaggat ctgccccgac cactgtatct
     3181 cgatcgagca gaccaagcgc gacgcggacg gctcgggcaa gcccaagccc ttcgcggtcg
     3241 ggttcgtgat cgacgactcg cggtgcatgt attgctccct gtgcgtcgag gtctgcccgg
     3301 tcaactgcat ctaccacacc gaggagttcg aggcacaggc ctacaaccgc ctggagctcg
     3361 ccaggcagtt cggtgagctc cccgtcgacc cgagggtcga ccccaaaccg accgtcaaga
     3421 aaaagaagaa gccaaccggc aagaaggccc ccaatacggt cgagaagccc aggaccgggg
     3481 gcgaagcggc ttgacggtag cgttcgcttt tctgaccggg atgacgctgg tctcggcgct
     3541 cggggtggtg ttgag
//
LOCUS       DBNGVD010000527         4170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3779964, whole genome shotgun sequence.
ACCESSION   DBNGVD010000527 DBNGVD010000000
VERSION     DBNGVD010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3779964"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="AC16CM_14225"
     CDS             complement(<1..1094)
                     /locus_tag="AC16CM_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3141038.1"
                     /translation="MPPYRARDGPDLDLSPPVRLDLWAVVLSAGIAVGTVAPPLAPVL
                     VVVSLLVAVASLLRRDLVTTEWRLMAVLGPLFVVVGFTIALVHAATEDPLAELAAMEP
                     GEVVMVGRLSSPPVPSSFGYMADFRVDHLWYHNREILRGGGVELFAGDLSVGVGDRVL
                     VEGEISRPQVGKDGFDYGRYLATKRISGLVEATSVRPVGEGRGWIGRIHRRTDVALGY
                     GLRPREAAVVRGMVLGDRSLMPEDLETAFQKSGVTHVLAISGQHVAILAAVIYYALRL
                     FAIPAPVRAGVTIGLVWPYILIAGAPPSAIRAGVVATFVLAAPLLGRQVSALHFMTTM
                     LALVLSYNPQLVYNTGFQLSVAAVFGILLLT"
     gene            complement(1081..1494)
                     /locus_tag="AC16CM_14230"
     CDS             complement(1081..1494)
                     /locus_tag="AC16CM_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3141039.1"
                     /translation="MRDELFETATRGAGARRRLPPNALGRYGPRLAVAVMVVMVLLGG
                     AFYTARLSEGTPTVVYTASLERVEDESQGSLKVDINTADVEELDELPEVGPSTAQSII
                     EYRQSNGGFSSVDQLEEIPGIGPTTLEKIEPFATV"
     gene            complement(1572..2345)
                     /locus_tag="AC16CM_14235"
     CDS             complement(1572..2345)
                     /locus_tag="AC16CM_14235"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR3141040.1"
                     /translation="MTVPVGLLSICVAMAVAVGGLILVQRFVPTDVRRQHNDVAGFIY
                     AVLGVTYAVLLGLMLVAVWEDWEAARISADTEASALAEIFWIADRLPAEDGQRVQELC
                     REYARAVVEDEWPLMQFGKSSPEAWALLDDIRANLQKVDPSTPGQQVIYEQSLERMRD
                     LADARRDRLLEAENGLPGILWVVLITGGVITVGFTYLFGLDSTIIHLLMVGSLALVLA
                     LVLFTVGALNFPFEGDITIQPDAMQHVLDRFRTSKLSDL"
     gene            complement(2473..2545)
                     /locus_tag="AC16CM_14240"
     tRNA            complement(2473..2545)
                     /locus_tag="AC16CM_14240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2510..2512),aa:Ala,seq:ggc)
     gene            complement(2596..2994)
                     /gene="rsfS"
                     /locus_tag="AC16CM_14245"
     CDS             complement(2596..2994)
                     /gene="rsfS"
                     /locus_tag="AC16CM_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3141041.1"
                     /translation="MSRDESVARETKFATEGEALTYEMAATAADAASEMFAREVVLID
                     LKGLVSYADYFVIASAETERQTRRIAQEVIERMIEKGHRPRTKRLDEGTAWISLDFID
                     VVVHIFTDEARDYYRLESLWRSAPQEHWQQ"
     gene            complement(2991..3641)
                     /gene="nadD"
                     /locus_tag="AC16CM_14250"
     CDS             complement(2991..3641)
                     /gene="nadD"
                     /locus_tag="AC16CM_14250"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3141042.1"
                     /translation="MDLKIGIFGGTFDPVHLGHMIIAEQVMAELDLARVIFVPGGIPP
                     HKEASSVRATAEERFAMVEAAVEGNERFSVDRVEIDSGRAMHSVETVGIFRERSPDDE
                     WFFITGADEVSNLLEWREPDRLLEQIVMVAATRPGYDLSRLDHLEAALRNFDRIFPVE
                     CTRVDISATGIRRRMLQGKSIRYLVPDGVHRIIESRRLYEGDGKRAEGGLLREEIR"
     gene            complement(3643..>4170)
                     /gene="cgtA"
                     /locus_tag="AC16CM_14255"
     CDS             complement(3643..>4170)
                     /gene="cgtA"
                     /locus_tag="AC16CM_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR3141043.1"
                     /translation="LLALVLDASEDPEGAEGTLRAELHAARLAAKPTVVVLNKVDLLD
                     AELRSYLREAFPEASQVSASTGEGVVELRDRFEEMLRGMGEQGGHDDTIERQHRVFRP
                     TWKGVRVEPENGGYVVSGEEVERLALKTDWGNAEGVERFQYELERTGVVGALRRAGAE
                     SGDEVRIGETVFDFQ"
BASE COUNT          728 a         1406 c         1304 g          732 t
ORIGIN      
        1 gttaggagga gtatcccgaa aacggcggcc acggagagct ggaagcccgt gttgtagacg
       61 agctggggat tgtaggacag cacgagggct agcatggtgg tcatgaaatg gagggccgag
      121 acctggcgcc cgaggagcgg cgccgcaagg acgaaggtgg ccacgacgcc ggcccttatc
      181 gccgagggag gggcgcccgc gatcagtatg tacggccaga cgaggccgat ggttactccg
      241 gcacgtacgg gggccgggat ggcgaagagc cgcagggcgt agtagatgac ggcggccagg
      301 atggcgacat gctggccgga gatggcgagg acgtgggtta cgccgctctt ctgaaaggcc
      361 gtctccaggt cttccggcat cagcgatcgg tcgccgagaa ccattccgcg caccacagca
      421 gcctcccgcg gcctgagacc gtacccgagt gccacgtcgg tgcggcggtg tatccggccg
      481 atccaccccc gcccctcccc caccggcctt acgctggttg cctcgaccag accgctgatc
      541 cgtttcgtgg cgaggtatct cccgtagtcg aagccgtcct ttccgacctg ggggcgggat
      601 atctcacctt ctaccagcac cctgtctcct acgccgacgc tcaggtcgcc ggcgaagagc
      661 tcgacgccac caccgcgcag aatctcccgg ttgtggtacc agaggtgatc caccctgaaa
      721 tccgccatgt agccgaagct gctcggcacc ggtggcgagg ataggcgtcc taccatgacc
      781 acctcccccg gctccatggc ggccagctcc gcgagcgggt cctcggttgc ggcgtgaacg
      841 agcgctatcg tgaagcccac caccacgaac aggggcccga gaacggccat caacctccac
      901 tcggtggtta cgaggtctcg cctcaaaagg gacgcgaccg cgacgagcag cgagacaacg
      961 acgagcacgg gggcgagagg tggagcgacc gtgccaacgg cgatcccggc ggacagcacg
     1021 acggcccaca gatcgagccg tacggggggg ctgagatcca ggtccgggcc atctctcgcc
     1081 ctatacggtg gcaaagggct ctatcttctc cagcgtcgtc ggtccgatgc cggggatctc
     1141 ctcgagctga tcgacggagc taaaaccccc gttgctttgc cggtactcga tgatgctctg
     1201 cgcggtcgag ggtccgacct cgggcagctc gtcgagctcc tccacgtcag cggtgtttat
     1261 gtccaccttc agcgagccct gcgactcgtc ctcgacccgc tccagggagg cggtatagac
     1321 gaccgtcggc gtcccctcag agagccgcgc cgtgtagaac gcgcccccga gcagcaccat
     1381 cacgaccatg accgccacgg ccagcctcgg cccgtagcgc ccgagagcgt tggggggcaa
     1441 ccttcgtctg gccccagcac cgcgcgtcgc cgtctcgaaa agttcgtccc tcacatggtg
     1501 ggtaatcggg cctggtgttc gaaaccttta gcgtgggccc ggcatgcggt gggatgtcgt
     1561 tacggggtgc gtcacagatc gcttagcttg ctggtgcgga acctgtcgag gacgtgttgc
     1621 atggcatcag gttggatcgt aatgtcgcct tcgaacggga agttcagggc accgaccgtg
     1681 aacaggacga gggcgagtac cagggccaga gatccgacca taagcaggtg gatgatggtg
     1741 ctatccaggc cgaagaggta ggtaaagccc accgtgataa cacccccagt tatgaggacc
     1801 acccacagga tgccgggcag accgttctcg gcttctagca gccggtctcg cctggcgtcg
     1861 gcgaggtcgc gcatgcgctc caggctctgc tcgtagatta cctgctgtcc tggtgtggac
     1921 gggtccacct tctgcaggtt ggccctgatg tcgtctagca gggcccacgc ctccgggctg
     1981 gacttgccga attgcatgag cggccactca tcctccacca cggcgcgggc gtactcgcgg
     2041 catagctcct gaacccgctg gccatcctcg gcgggcagcc tatcggcgat ccagaagatc
     2101 tcggccagcg cgctcgcctc cgtgtcggcg ctgatcctgg ccgcctccca atcttcccac
     2161 acggcgacca gcatcaggcc gaggagcacc gcgtaggtca ccccgaggac cgcgtagatg
     2221 aagccagcga cgtcgttgtg ctgccggcgc acgtcggttg gcacgaaccg ctgtacgagg
     2281 atcaggcctc cgacggcgac ggccatggcc acacatatgg aaagcaaccc gacgggtaca
     2341 gtcatggcgc gctaccctcc tacggttcga gattcccggg ctggtcaaga tacaatctgg
     2401 tcggtccgga agcaaaccct tcgggggcac ggcacaacgg tggatgggat gactggggct
     2461 actgcgtggg ggtggagccg agggggatcg aacccctgac ctccgcgctg ccagcgcggc
     2521 gctctcccag ctgagctacg gccccgtaaa aagcgctcag agtatatgca gcatggcctg
     2581 gggtgtcaac gtgggctact gctgccagtg ctcctggggg gcgctgcgcc agagggattc
     2641 gaggcggtag tagtcacgtg cttcgtcggt aaagatatgc acgaccacat cgatgaagtc
     2701 taggctgatc caggccgtac cctcgtcgag gcgcttggtg cgcgggcggt ggcccttctc
     2761 gatcatgcgc tctatgacct cctgggcaat gcgccgcgtc tggcgctcgg tttcggcgct
     2821 ggctatcacg aagtagtcgg cgtaggagac gagccccttg aggtcgataa ggaccacctc
     2881 cctcgcgaac atctcactcg ccgcatcggc ggccgtcgcg gccatctcgt acgtcagcgc
     2941 ctcgccctcg gtggcgaact tggtctctct tgcgaccgac tcgtcgcgac tcaacggatc
     3001 tcctccctca aaagccctcc ttctgcacgt tttccgtcgc cctcatacaa cctcctgctc
     3061 tcgatgatcc tgtgcacccc gtcgggtacg agatacctga tgctcttgcc ctgcaacatc
     3121 ctgcgcctta taccggtggc cgagatgtct acccgcgtac actcgaccgg aaagatcctg
     3181 tcgaagttcc tcagggcggc ctcgaggtgg tctaacctgg aaaggtcgta ccccggtcgc
     3241 gtggcggcta ccatgactat ctgctcgagc agccggtccg gctccctcca ctcgaggagg
     3301 ttcgacacct cgtctgcgcc ggttatgaag aaccactcgt cgtcgggcga acgctccctg
     3361 aaaatgccaa cggtctccac gctgtgcatg gcgcgtccgg agtcgatctc gaccctgtcc
     3421 acgctgaacc tctcgttgcc ctcgacggcg gcttcgacca tggcgaacct ctcctcggcc
     3481 gtggccctga cgctggaggc ttccttgtgg ggcgggatgc caccaggcac gaaaatgacc
     3541 ctggcaaggt caagctcggc catcacctgc tcggcgatga tcatgtgccc caggtgaacg
     3601 gggtcgaagg ttccgccgaa gatacctatc ttcaagtcca cctcactgga agtcgaacac
     3661 cgtctcgcct atcctgacct cgtcgcccga ctcggccccg gcccgccgga gggccccgac
     3721 gacgccggtc cgctccagct cgtactggaa cctctcgaca ccctcagcgt tgccccagtc
     3781 ggtcttgagc gcgagccgct cgacttcctc cccggataca acgtagccgc cgttctccgg
     3841 ctcgaccctg acgcccttcc aggtcggcct gaagacgcga tgctgccgct cgatggtgtc
     3901 gtcgtgaccg ccctgctccc ccatcccgcg cagcatctcc tcgaacctgt cgcgcaactc
     3961 gacgacacct tcgcccgtgg aggcggagac ctgcgaggcc tccgggaacg cttcccgaag
     4021 gtacgaccgg agctctgcat cgagcaggtc caccttgttc agaaccacga ccgtgggctt
     4081 ggcggcgagc cttgccgcgt ggagctcggc ccgcagcgta ccctccgccc cctccgggtc
     4141 ctcgctcgcg tcgagaacga gggcgagcaa
//
LOCUS       DBNGVD010000528         3353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3234052, whole genome shotgun sequence.
ACCESSION   DBNGVD010000528 DBNGVD010000000
VERSION     DBNGVD010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3234052"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16CM_14260"
     CDS             complement(<1..236)
                     /locus_tag="AC16CM_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141044.1"
                     /translation="MSEDPRDPLAGLAEGMEAAGALIESLEGEVESLRTDVEEAYAAL
                     KAAQEEVSSRADELQEKERDRIEATRKAEGLQEE"
     gene            complement(373..831)
                     /locus_tag="AC16CM_14265"
     CDS             complement(373..831)
                     /locus_tag="AC16CM_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3141045.1"
                     /translation="MVTDRPTLHDGVSDAPSVREKLRRSGYTLTSQRRAVLEALSRAK
                     GHPSAEDVYLIVKRENPRVALGTVYQALSVLEEVGVIGSKHWSESPTRYDLNVEPHLD
                     IRCTSCGEVAEIPGVDLAGAEASIRENTPYEVTRATMVVEGLCPGCASNQ"
     gene            891..2585
                     /gene="sppA"
                     /locus_tag="AC16CM_14270"
     CDS             891..2585
                     /gene="sppA"
                     /locus_tag="AC16CM_14270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3141046.1"
                     /translation="MSLILDALANLWRLVRNARVRLLARPPDFVWVEVGGPLDEFETP
                     VGFLRRRLSPGPTPPTLERLRARLDLISSDGRPRGIILRIKNLDAGWAAIEELRGEIL
                     AFRERGHRVVAYLGDPVDTRSYYLACAADEILATPLADLNVTGLRARVDFIKDALDNF
                     GVEVEVVAVSPYKSAGERFVRDDFSEESREQVGRLLDRRYGEVVRAISQGRGISYREA
                     ERKIDLAPYGARGALSEGLIDGVLYEDELPHRLGSGAGRARLAEWPRAKRSLRIPYRR
                     RSRKKVALVSLTGTISRGRSRRLPVPLPFAGREQAGSESVVAALRLAERNPRVSAVLL
                     HVDSPGGDALASDLIWREVERTNTKKPVVVLMGNAAASGGYYVSVPARHVIARGGTVT
                     GSIGVLTIRPIASGLYEKLGINPAAIERGERAGLLDLSRRPDDEERRVVEGQIQRVYD
                     EFKDRVARGRDIDAAELEPMAGGRVWTGEEALELGLVDEIGGFRQALRRARELGGIQR
                     DVPEVLLRITPPRSERPTPGEPAQVLIELVGEVWGGVSDLGAGGVRLLTPYEIRED"
     gene            2629..>3353
                     /locus_tag="AC16CM_14275"
     CDS             2629..>3353
                     /locus_tag="AC16CM_14275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141047.1"
                     /translation="MSEVSRKGVDRRAMAGLSAGHLFTDLNQGAVAALVPFLTAERGI
                     SLAAAGALVFAATVSSSIVQPLFGIFSDRRPIPALMPLGVLLAGCGMALVGVAPSYPL
                     ILLCVVASGIGVAAFHPEAARFANYVSGSGRARGMSFFSVGGNAGFALGPAVTTPLVL
                     LFGLKGSLFLALPALAMAAVLVHELPRMLSFRPEPGETEGEADVEMPEHWGPFSRMIA
                     VVVVRSFVYFGLVAFVASYYERVL"
BASE COUNT          513 a         1058 c         1140 g          642 t
ORIGIN      
        1 atctcctctt gcaggccctc tgccttgcgg gtcgcctcga tgcggtcccg ctccttctcc
       61 tgtaactcgt cggcccgcga ggatacctcc tcctgggccg ctttcagggc cgcgtaggcc
      121 tcttccacgt cggtcctgag gctctctacc tctccttcga ggctttcgat cagggctcca
      181 gcagcctcca tgccctccgc gagcccggcc aacgggtctc tgggatcctc gctcatgttc
      241 ggttttcctg taatacccaa atcaccacct tgccggaagg ttaacgcctg gcaagcgcct
      301 ggtaaagtct agattcctac gagttcggcc gcgtgcgatc gtcataaaca tcgacaggac
      361 gggcttggat gcttactggt tgcttgcgca gccggggcag agaccctcga ctaccatcgt
      421 cgcgcgcgtg acctcgtaag gggtgttctc cctgatgctt gcctccgcgc cggcgaggtc
      481 tacgccgggg atctcggcta cctcgccaca gctcgtgcac cggatgtcca ggtgtggctc
      541 gacgttcagg tcatagcgcg tgggtgactc ggaccagtgt ttggagccta ttaccccaac
      601 ctcttctagc acggagaggg cctggtagac cgtgccgagc gccacccgcg gattctcacg
      661 cttgacgatc aggtagacgt cctcggcgga aggatgtccc ttggccctcg acagggcctc
      721 cagaaccgcc ctgcgctggg aggtcagcgt gtaccccgac cggcgcaact tctcccttac
      781 ggagggtgca tcggagacgc catcgtgaag tgttggacga tcagtaacca ttcctgatct
      841 cgagtttatc atacggcgtt ttacgcgttg gggtgtacca taaccaccag atgtcgctta
      901 tcctcgatgc cctggcaaac ctgtggcgcc tggtacgcaa cgcccgcgtg cgtctgctcg
      961 ccagaccgcc ggacttcgtg tgggtggagg ttggcggccc gctggacgag ttcgagacgc
     1021 ccgtcggttt tctccggcgc cggctctcgc ctggtcccac cccgcctacc ctggagcgtc
     1081 tgcgcgccag gctggatctc atctcctcgg acggccgtcc ccgtggcatc attctgcgta
     1141 tcaagaacct ggacgccggc tgggctgcca tcgaggagtt gcgcggcgag atcctggcct
     1201 tcagggaacg gggacaccgg gtggtggcgt accttgggga tcccgtcgac acccgttcct
     1261 actacctggc gtgcgcggcg gacgagatac tcgccacccc gctcgccgac ctgaacgtca
     1321 cggggctacg tgccagggtg gacttcatca aggacgcact cgacaacttc ggggtggagg
     1381 tagaggtggt ggccgtctcg ccgtacaagt cggcgggcga gcggtttgtg cgcgatgact
     1441 tctccgaaga atcccgcgag caggtcgggc gtctgctcga ccgccgttac ggggaggtgg
     1501 tgcgcgccat ctcccaggga aggggcatct cctaccggga ggcggagagg aagatagatc
     1561 tggcccccta cggcgcgcgg ggtgccctct cggaagggct catcgacggc gttctctacg
     1621 aggatgagtt gccgcacagg ctggggtccg gggccgggag ggcccggctc gccgagtggc
     1681 cccgggcgaa aaggtcacta cgcatccctt accggaggcg gagcaggaag aaggtcgcgc
     1741 tggtgagcct gaccgggacc atctcccggg gaaggagcag gaggctaccg gtcccactcc
     1801 cattcgcggg aagggagcag gcagggagcg agtccgtggt ggcggcattg cggctcgctg
     1861 agagaaatcc gagggtctcc gccgtccttt tgcacgtcga ttcgcccggc ggcgacgcgc
     1921 tggcatcgga cctgatctgg cgcgaggtcg agcgcacaaa cacgaagaaa cccgtggtgg
     1981 tcctgatggg aaacgccgcg gcttcgggcg gctactacgt ctccgtcccc gcacgccacg
     2041 ttatcgcccg tggcggcacg gttaccggct ccataggcgt gttgaccatt cggccgatcg
     2101 ccagcggcct ctacgagaag cttggcatca atcccgccgc gatcgagagg ggggagcgcg
     2161 ccggactact ggacctcagc cgccgccccg acgatgagga gcgacgggtt gtcgagggcc
     2221 agatccagag ggtctacgac gaattcaagg accgggtcgc ccggggacgc gacattgatg
     2281 ccgctgaact ggagcccatg gctggcggca gggtgtggac cggtgaggag gccctcgagc
     2341 tcgggttggt ggatgagatc gggggcttcc ggcaggcgtt gcgcagggcc agggagcttg
     2401 gtggaataca acgcgacgtg cctgaggttc tgctaagaat cacgccgccc cgcagtgaaa
     2461 ggccgacccc gggcgagccc gcgcaggtgc tcatcgagct ggtaggtgag gtatggggag
     2521 gtgtatccga ccttggcgcg ggcggtgtca ggttgctaac gccgtacgag attcgggaag
     2581 actgatagca tctgcgcctg tgggccgcgg gctggaagga agcgcgactt gagcgaagtc
     2641 tccagaaaag gcgttgaccg gagggccatg gccggactgt cggccgggca tctcttcacc
     2701 gacctgaacc agggcgcggt cgcggcgctg gttcctttcc tgacggcgga gcgcggtatc
     2761 tcgctcgctg cggccggcgc gctcgtgttc gcggccacgg tcagctcctc gatcgtgcag
     2821 ccgctcttcg gtattttctc ggacagaagg ccgatcccgg cgctaatgcc gcttggcgtc
     2881 ttgctcgcgg ggtgcggcat ggcactagtc ggtgtggccc cgagttatcc cctgatcctg
     2941 ctctgcgttg tggccagcgg gatcggggtg gccgccttcc acccggaggc ggcgcggttc
     3001 gcaaactacg tctcgggttc cggccgggcc cgcgggatga gcttcttctc cgtgggtggc
     3061 aacgccggct tcgccctcgg accggcggtg accacgccgc tcgttcttct gttcggactc
     3121 aaaggttcac tcttcctcgc tcttcccgcg ttggcgatgg ccgccgtgct ggtccacgag
     3181 cttccccgga tgctgagctt ccggccggag ccgggcgaga cggagggcga agcggatgtc
     3241 gagatgccgg aacactgggg acccttctcg cgcatgatcg cggtggtggt cgtgaggtcg
     3301 ttcgtctact tcggactggt ggccttcgtt gcctcctact acgagcgcgt gct
//
LOCUS       DBNGVD010000529         9527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3416381, whole genome shotgun sequence.
ACCESSION   DBNGVD010000529 DBNGVD010000000
VERSION     DBNGVD010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3416381"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="AC16CM_14280"
     CDS             complement(<1..158)
                     /locus_tag="AC16CM_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141048.1"
                     /translation="MNHPQQASADRDRRRATPYGKILVATDGSDCSMLAAAHAIYLAE
                     SLGARLYAV"
     gene            complement(306..1262)
                     /locus_tag="AC16CM_14285"
     CDS             complement(306..1262)
                     /locus_tag="AC16CM_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141049.1"
                     /translation="MGLRGSWLGEAGAGKERTVPFPPGRILLATDGSQDAALAARAAV
                     DLSSMVGCELHVAHFWQTVPTTRFEAMVRAQLRLEAQELLEAQEASLREAGAEVAGAH
                     LRLASAVDGVLDLAEELGADLLIMGSRGRGPVKRLILGSVSEGVVHQARVPVLVVRGG
                     DDAWPPESIVIGDDGSEAAANAACLAMGIGGLFGAGGLLVRTYPRLPEVDPEGRALDA
                     RMVDDELRREENILEKRARQLRENLGARSRVRIDVGDPAARLLGAAEERHEGERTLLA
                     VGSRGLGPLGRARLGSVSTKVLRAAKGPVLICPAPRACGPQA"
     gene            complement(1253..1402)
                     /locus_tag="AC16CM_14290"
     CDS             complement(1253..1402)
                     /locus_tag="AC16CM_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141050.1"
                     /translation="MGIGQIPIIEGAFRLVEDRLALRRLRDEAPKPDAGASRNELWAE
                     ATIWA"
     gene            complement(1458..2723)
                     /locus_tag="AC16CM_14295"
     CDS             complement(1458..2723)
                     /locus_tag="AC16CM_14295"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3141051.1"
                     /translation="MQGSTRPNVTVLGGGFGGLEAAFYLRMKLGDEANITLVSDRDYF
                     LFKPNTIYIPFGLDPDRLKIDLGRPTRRKDITFVKDRVREVDPDGKTVSTEGRKLAYD
                     YLVVATGSGMRPEEVPGLAENARTIWTPREMLELRLALYDLLEAAEEGKLKRVLFLVA
                     PNNKCAGPLYELVMMLDTWLRRKQVRANMDLTWSTYEQGYIQAFGPRLHEVVEREFER
                     RVIAGHTGYVAETVEDGAVRYANGEELPYDLLITFSPYVSEMTYPTLPGDERGFITTD
                     LASRQVVGQPDVYAVGDAGDFPVKQAFLAFMQADAAAEHLSARLLGTEPEFGFEPTSM
                     CVMEEFDKATFAQVPLRLTGEPERPVEVDPEAIAAYKVGTSVPWRLGKKMIGMYLPLR
                     FGWGNPFHAGLPWKGMEVGLKAMSGLMAR"
     gene            complement(2982..3965)
                     /locus_tag="AC16CM_14300"
     CDS             complement(2982..3965)
                     /locus_tag="AC16CM_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141052.1"
                     /translation="MFSTKVLLATDGSAEAERAARMAKMVSENLGAELHVVHVAPTPD
                     PTAWPEATIYDRDLLMQSREQAEIEGRPVLDRQRHYLEAMGAETAVPHLLVGRPDAEI
                     VRLAEELGSGLIVIGSRGIGPVRRAVMGSVSGSVLRHAHCPVMVVRGKDEEEMTAVGP
                     IVLAVDGSEEAQLATIAAAEFSRGTGSEVHLVAVLPELASMFGRHYYSTDIKEMLLEQ
                     VDADSRAFLDKQAEAVRSRGGSPAQTYLARGRPDAEIVELAEEIGAGLIVLGNRGLGG
                     VKRALMGSVSASVARHAHCPVLVVRTAKDRDKRDTPASEKPIEVRAPGSGR"
     gene            complement(4018..4320)
                     /locus_tag="AC16CM_14305"
     CDS             complement(4018..4320)
                     /locus_tag="AC16CM_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141053.1"
                     /translation="MAERRGADTRRVTQAATDRRELWRDVQEAHAALRGEHQIAEYVA
                     ALDIGECARILASDPEGLPRLAALRDECRVAREESRTFEQVIRTWTLEVLSGAEGG"
     gene            complement(4589..5284)
                     /locus_tag="AC16CM_14310"
     CDS             complement(4589..5284)
                     /locus_tag="AC16CM_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR3141054.1"
                     /translation="MVERVERATEKHDEGIAEAAPYHTPYFTTAGLLLSVAGAIILMG
                     IITAEALYPAPYDTAANTISDLGGTMPSEGGIVLQPSATIFDATMILAGAMIIVGSLF
                     VYRATERWGATIPLALLGTGVLGVGIFPGHVPVWHPIFALLAFVAGGVAAVMAYEVAT
                     PPFRYVSATLGVITLVSIVLGFFFLETLDFVASLGEGGIERWIAYPVVLWLTMFGGYL
                     MGHARRRPPGVRP"
     gene            complement(5552..6454)
                     /locus_tag="AC16CM_14315"
     CDS             complement(5552..6454)
                     /locus_tag="AC16CM_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141055.1"
                     /translation="MSIFPARILLATDASREAEFAVRAARELANETGSELHVVHVLPL
                     PIDTQDPSSFEPEVRRRFEERTRARLDETVGVIHESGGKVAESHLRTGRPDAEIVDLA
                     EEISAGLIVMGSRGLGPARRSLLGSISDSVVRHAHCPVMVVRGAALAFPTRILLATDG
                     SAEANIAASTAADLANSTASELHVVCVGRMPMVFYESPGVTMQEPELQDRTVKDAEQA
                     TRTRLEELVNELGEAGGEVTEAHARVGIADTEIVGLAGSLGAGLVVMGSRGRGPLRRA
                     LMGSVSDSVVRHAPCPVLVVRPRY"
     gene            6966..8006
                     /locus_tag="AC16CM_14320"
     CDS             6966..8006
                     /locus_tag="AC16CM_14320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3141056.1"
                     /translation="MMSVGQQEEVLRTLSEVFADRVKPVPPGDGPGDALASVRPSSVE
                     EVSYLAQVAARQSLPLVPLGAETGFGPSPPQGSILVRFDLMRSLRLPENDEPWVEAGP
                     GTSWLNLENELRVRGRGLTVYPTSAPRATVGGWLAIDGLGVGSFEFGRLWKNVLRADV
                     VMHGGGLHEVEGEELGHLFGPGKAHRIVVNARLRTRRAKGDRPFAVAFSEPASVAGAV
                     TALLASEAPLWHLAFVSPTMAAARGLREQYLLFGAYPGARDGEEWWDLRGRVIDDHGG
                     VELDGHEAWRIWGERFFPVAPAHPTPHATRRFISAGRLTQELEDSGPGDAFQGTVGSS
                     GEVLLLKLGVGE"
     gene            8443..>9527
                     /locus_tag="AC16CM_14325"
     CDS             8443..>9527
                     /locus_tag="AC16CM_14325"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR3141057.1"
                     /translation="MSAAVTIFQMALLAGIVSRVFLEGAGIGGLTTWLVLLVCAVFLR
                     AGLVWAREVVAERGAVRVKAEIRERLFSHILRLGPAYAAGERAGELTTTATEGVERLE
                     PYFAHYLPQALLSVLVPVMVAAYVATQDLTSAVLLLATAPVIPVMMVLVGSYAEEHTR
                     RQWTALSRLGAHFLDALQGLPTLKAFGRARNEARRLAETGEEFRGRTMKVLGFAFLSG
                     LVLEFMTSAAIALLAVMLGVRLIGGAVAFEQAFLVLLLAPEFYRPLRELGVHRHAGME
                     GKAAAERIRGILQTPAGADIVPGDFRPFRAVEVEISEVGFSHPEGSRHVLDGVSMTLP
                     VGSITALVGRSGAGKSTLVNLLLRFLEP"
BASE COUNT         1523 a         3246 c         3017 g         1741 t
ORIGIN      
        1 accgcgtaga ggcgggctcc gagcgactcg gcgaggtaga tcgcgtgggc ggccgcaagc
       61 atggagcagt cggacccatc cgtggccaca aggatcttcc cgtaaggtgt cgccctgcgc
      121 ctgtcccggt cggcgcttgc ttgctgtggg tggttcatct ccgtttctcc tctcgctcgc
      181 tgcccttcca cctttcatcc gtccgtaact actccaggcg tacgagggtg cgcccgcctc
      241 tcccgttacc accagggccg atggcaggtg ggtccgccgc ggctggggag gggtactccc
      301 gacgtctagg cctgcgggcc acaagctcgc ggggctgggc agatcagcac gggccccttc
      361 gccgccctga gaacctttgt agagacgcta ccgagcctcg cgcgtcccaa aggcccgagc
      421 ccccgacttc ccacagcgag gagcgtccgt tcgccctcgt gtcgctcttc ggctgcaccg
      481 agcagccgtg cagcggggtc tccgacgtct attcggaccc tggatcgggc accgaggttc
      541 tccctcaact gcctggctcg tttctcgagg atgttctcct cgcgccgcag ttcgtcgtcc
      601 accatgcggg cgtccagggc acgcccttcg gggtccacct ccggcagcct ggggtaggtc
      661 ctgacaagga gcccgccggc cccgaagagc ccgccgatcc ccatggccag gcaagcggcg
      721 ttggccgccg cttccgatcc gtcgtccccg atcacgatac tctcgggtgg ccaggcatcg
      781 tcgccgcccc gtacaacgag cacgggaacg cgggcctggt ggaccacgcc ctccgagacg
      841 ctgcccagga tgaggcgctt gacgggtccg cgtccccggc tgcccatgat caggaggtcg
      901 gccccgagct cctcggcgag gtcgagcacg ccgtctaccg cgctggccaa ccggaggtgc
      961 gcgccggcga cctccgcgcc ggcctcgcgg agagacgcct cctgcgcctc gagcagttcc
     1021 tgcgcctcca gccggagctg ggcccgcacc atcgcctcga agcgcgtggt cggcacggtc
     1081 tgccagaagt gtgcaacgtg caactcgcaa ccaaccatgc tcgagaggtc tacggccgcc
     1141 cgggccgcaa gcgcggcgtc ttgcgagccg tcggtggcga gcagtatcct ccccggaggg
     1201 aagggtacgg tcctctcctt ccccgcaccc gcctcgccga gccaggagcc cctcaggccc
     1261 atatcgttgc ctcagcccac aattcgttcc tgctggcccc ggcatctggc ttcggggcct
     1321 catcccggag gcgccgcaag gcgaggcggt cctcgaccag ccggaaggca ccttcaataa
     1381 tgggtatctg acctatgccc atcctggccc ccttcctcta caccctggcc cctgctgctg
     1441 cggctccaca cgtcccgtca cctggccata aggccactca tcgcctttag ccctacctcc
     1501 attcccttcc aggggagccc ggcgtggaac gggttacccc acccgaaacg caggggcagg
     1561 tacatcccga tcatcttctt cccaagccgc cacggcacag aggtgccgac cttgtaggcg
     1621 gcgatcgcct cggggtcgac ctccacgggc ctctccggct cgcccgtcag cctaagtggg
     1681 acctgggcga aggtggcctt gtcgaactcc tccatgacgc acatgctcgt gggctcgaaa
     1741 ccgaactctg gctcggtccc tagcaggcgg gcggagaggt gctcggcggc ggcgtcggcc
     1801 tgcatgaagg cgagaaaggc ttgttttacg gggaagtccc cggcgtcacc cacggcgtag
     1861 acgtcgggct gcccgacgac ctgcctgctc gccaggtccg tcgtgatgaa gcccctctcg
     1921 tcgccgggaa gcgtggggta ggtcatctcc gagacgtagg gggagaaggt gatgaggagg
     1981 tcgtagggga gttcctcccc gttcgcgtac cggacagcgc cgtcctcgac ggtttcggcg
     2041 acgtagcccg tgtggcccgc tatgacgcgc cgctcgaact cccgctccac gacctcgtgc
     2101 agcctggggc cgaaggcctg tatgtagccc tgttcgtagg tggaccatgt caggtccatg
     2161 ttggccctga cctgcttgcg cctgagccag gtgtcaagca tcatgaccag ctcgtagagg
     2221 gggcctgcgc acttgttgtt gggcgctacg aggaacagca ccctcttgag cttaccttct
     2281 tcggccgcct ctagcaggtc gtagagggca agcctgagct ccagcatctc ccggggcgtc
     2341 cagatggtcc tggcgttctc cgcgaggccg ggcacctcct ccgggcgcat acccgaaccg
     2401 gtcgccacga cgaggtagtc gtaggcaagc ttccggccct ctgtagagac cgtcttgccg
     2461 tcggggtcca cctcacgcac cctatccttg acaaaggtaa tgtccttgcg gcgtgttgga
     2521 cgcccgagat ctatcttcag cctgtccgga tccaatccga aggggatgta gatggtgttc
     2581 ggtttgaaca ggaagtaatc cctgtccgag acgagcgtta tgttagcctc gtctccgagc
     2641 ttcatcctca ggtagaaggc tgcctcgagc ccgccgaacc cgcccccgag gaccgtcacg
     2701 ttcggtctgg ttgacccctg catggtccac gctcctctcc cgtccgtccc ggccccggta
     2761 agatctacgg catcgtctcg gacggcctgt ctgctctctc tactgtactt ccaagggtcc
     2821 tatgagccct gacccaggta gcccattctg ctggtcagga ttgaccagag cggccgccac
     2881 agagggcctt caggggatga gatgaggact tcggggggac gcaggaggac ggcccgcggc
     2941 agcgcccgtc ttcgagacac tcggggctac cctcagaggg cctaccgtcc ggagccaggc
     3001 gcccggacct cgatcggctt ctcggacgcc ggggtgtcgc gtttatctcg gtccttcgcc
     3061 gtcctgacga ccagaaccgg gcaatgggcg tggcgggcga ccgaagcgga cacgcttccc
     3121 atcagagccc gcttcacacc cccgagtccc ctgttgccca gtacgatcag gccggctccg
     3181 atctcctcgg caagctctac gatctcggcg tccggacggc ccctggcgag gtaagtctgg
     3241 gccgggctac ctcctctgga gcgcacggcc tcggcctgct tgtcgagaaa ggcgcgggag
     3301 tctgcgtcga cctgttcgag tagcatttcc ttgatgtcgg tggagtagta gtggcgaccg
     3361 aacatgctcg ccagctccgg cagtacggct accaggtgca cctccgagcc cgtgccccgg
     3421 gaaaactcgg ccgccgctat cgtggctagc tgggcttcct cggagccgtc gacggcgagc
     3481 actatgggcc ccaccgcggt catttcctct tcatcctttc cacgaaccac catgacgggg
     3541 cagtgggcgt gccggagaac gctacccgag acgctcccca tcacggcgcg tcttacgggc
     3601 ccgatacccc ggctaccaat gacgatcaaa cccgagccga gctcttctgc cagtcggact
     3661 atctccgcat ccgggcggcc caccagcaga tgcgggaccg cggtctcggc ccccatcgcc
     3721 tccagataat gcctctgcct gtcgagtacc ggtcggccct cgatctcggc ctgctctcgt
     3781 gactgcatga gcaggtctcg atcatagatg gttgcctcgg gccaggccgt cgggtctggc
     3841 gtcggcgcca cgtggacgac gtgcagctcg gcgccgaggt tctcggatac catcttcgcc
     3901 atccgggctg cccgctcggc ctccgcggac ccgtcagttg ccagcaaaac cttcgtagaa
     3961 aacatcgctc ctccttctgc gctcgtcgcc cggtgccgca cccgatgcct ccgtggttca
     4021 gccgccctca gcccccgaga gcacctcaag cgtccaggtc ctgatcacct gctcgaaggt
     4081 gcggctctcc tcccgggcca ctcggcactc gtccctgagg gcggcgagcc ttggaagacc
     4141 ctcagggtca gaggcgagga tcctggcaca ctccccaata tccagggctg ccacgtactc
     4201 tgcgatctgg tgctctcccc tgagagccgc gtgcgcctcc tgcacgtcgc gccagagctc
     4261 gcgcctgtca gttgccgctt gtgtgactcg tctcgtgtct gctcccctgc gctcggccac
     4321 ggcttacccc cgtcgtgctt tgtcgtgtgt cccaagagaa atgtattgca ggcacccacc
     4381 ggtgccctga ccagtatggt ttatgcgact ggcaaggaag tgccaacgct tcttcatctc
     4441 cgcctccgtg caagttcggt atcccagagg cgcaccacct gccccgcgtg cgagcgtgga
     4501 actaatactg tgtttgctcg ccgggtcacc agagccttca cgcggctccc tttccccccg
     4561 ccagctgtcg cttctcgcca gggtcgggtc aaggccgcac gcccgggggc cgtcgccgcg
     4621 cgtggcccat gaggtagcca ccgaacatgg tgagccacag cacgaccggg taggctatcc
     4681 acctctcgat ccctccctcg cccagggacg caacgaaatc caacgtctcg agaaaaaaga
     4741 acccgagaac tatactcacc agcgtgatca cgcccagggt cgccgaaacg tatctgaaag
     4801 gcggtgtcgc gacctcgtag gccatcacgg ccgccacacc gccagccacg aacgcgagga
     4861 gcgcgaagat cggatgccac acagggacgt gaccgggaaa gataccgacg ccaaggacgc
     4921 cggtgcccag gagggctaga ggtatcgtag ccccccacct ttcggtcgcc cgatagacga
     4981 aaagcgaacc gacgatgatc atcgcaccgg ccaggatcat ggtggcgtcg aagatggtcg
     5041 ccgatggctg caagacgatg ccgccctcgg acggcatggt gcctcccaga tcgcttatcg
     5101 tgttcgccgc ggtatcgtac ggggcgggat acagggcttc ggcggtgatg atgcccatca
     5161 ggatgatcgc ccccgcaaca gacagcagca agcccgccgt cgtgaagtag ggcgtatggt
     5221 acggtgcagc ttctgctatc ccctcgtcgt gcttctccgt agccctttcg actcgctcga
     5281 ccatggttcg cctcctggcg ggcccgcgtc tcgtagccga gaatagcagc ggcagaccgt
     5341 gctttcttga gcaaaagtgc ccaactatgc aagccatatt ggccagctac gatcccaaga
     5401 tcggccgtcg ttcatctccc cggccctatg gctagcgtcc cgtagtcttt gcgaccggca
     5461 acgtctaccc tggcgtttcg cttctctgtt cggtggtgct cggcgaggcc gcacagtctt
     5521 gttcgtggca tcccttcgac ttcagcgggg ctcaataccg cgggcgcacc acgaggacgg
     5581 gacacggggc gtgcctgaca acggaatccg agacgcttcc catcagcgcc cgcctcagcg
     5641 gcccgcgtcc tcggcttccc atgactacca gcccggcacc caggctacca gcgagaccca
     5701 cgatctccgt gtcggcgatc ccgacccgcg cgtgggcttc ggtgacctcg ccgccggcct
     5761 cgcccagctc gttcaccagc tcctctagcc tggttctggt cgcctgctcg gcgtccttca
     5821 ccgtcctgtc ttgaagttcg ggctcctgca tggtcacccc gggggactcg tagaagacca
     5881 taggcatgcg tcccacacag acgacgtgca attccgaggc cgtgctattc gccagatccg
     5941 ctgcagtgga ggcggcgata tttgcctccg cggatccgtc ggtcgcaagg aggatcctgg
     6001 tcgggaaggc gagtgccgct ccgcgtacca ccatcaccgg gcagtgcgcg tgcctcacga
     6061 cggagtccga gatgctgccc aggaggcttc ttctcgcggg cccaaggccc ctgctcccca
     6121 tcacgatcag gccggcactg atctcttcgg ccaggtccac gatctccgcg tccgggcggc
     6181 ccgtcctgag gtgagactct gccacctttc ccccggactc gtggatcacg ccgaccgtct
     6241 catccaacct cgcgcgcgtt cgctcctcga accggcgccg gacctcgggc tcgaaactcg
     6301 aagggtcctg ggtgtctatt ggcaggggaa gaacgtgaac cacgtgcaac tccgagcccg
     6361 tctcgttggc cagttcgcgg gccgccctga ccgcgaattc cgcctccctg gaggcatcgg
     6421 ttgccagcag tatcctggct ggaaagatgc tcatctctta tcgtacctcc ttaagcacgg
     6481 gtctgaagga tcgagccgtt ctggaggccc cgggcgaacg aggcaggccg aacgctcgtc
     6541 ccggggggcg cgatcccctc ctcagccgat caaccatcca gtacctcctc gcacgacctc
     6601 tggttccggg tgcttttgtc ttgcacatcg atgctagcgc cccatgcgcc tggtgccttg
     6661 aacacgactg cccaatcggc tgggttatct tgatcatctt ccggcaatgc cgttcttatg
     6721 atactatgat tacaataagg aggaggtccc cgtggaatgc gagcgggtca ggcacatccg
     6781 ggcatacctt cgagtgaacg cgtcctccgc agcgtttcgg agaaaacgcc actggcgggc
     6841 tcgtccggac gctgtggggc aggtcaaaat gggcaatctt gtcctgccat gacgtcaaaa
     6901 tggatcatgg caaaccatct cactggtcct atcttgttgc tgaatgcagc caaggaggaa
     6961 ggtacatgat gagtgttggg cagcaagaag aagtgcttag gaccctgagc gaggtcttcg
     7021 cggaccgcgt gaagccggtg ccgcccgggg atggaccggg cgatgccctg gcctccgtgc
     7081 ggccttcgag cgtggaggag gttagctacc tcgcgcaggt agccgcgcgc caatccctac
     7141 ccctggtccc actcggggcc gaaacgggct tcgggcccag tccgccacag ggaagcatcc
     7201 ttgttcggtt tgacctgatg cgctcccttc gcctgccgga gaacgacgag ccctgggtcg
     7261 aggccgggcc tggaacgtcc tggctcaacc tcgagaacga gctgcgcgtc cgcggtcgtg
     7321 gactgaccgt ttaccctacg agcgcgccga gggccaccgt gggaggctgg ctcgccatcg
     7381 acgggctcgg cgtgggctcc ttcgagtttg gccggctctg gaagaacgtc ctgcgtgccg
     7441 atgtggtgat gcacggtggc gggctgcacg aggtcgaggg cgaggagctt ggccacctgt
     7501 tcggcccggg taaagcccac cgcatcgtcg ttaacgccag gttgaggacc cggcgcgcaa
     7561 aaggggatag gcccttcgcg gtagccttct cggagcccgc cagcgtggcc ggggcggtta
     7621 cggcgctact cgcctccgag gcgccgctat ggcatcttgc cttcgtaagc cccacgatgg
     7681 ccgccgcccg gggactcagg gagcagtacc ttctcttcgg ggcgtacccg ggggccaggg
     7741 acggagagga gtggtgggac ctgcgtgggc gtgtgatcga cgaccacggt ggcgtcgagc
     7801 tcgatggcca cgaggcctgg cgcatctggg gagagaggtt cttccccgtg gcgccagcac
     7861 acccgacccc ccacgccacg agacggttca tctccgccgg gaggctcacg caagagctcg
     7921 aggattcggg gccaggggac gccttccagg gcacagtggg cagttcggga gaggtgttgc
     7981 tgctgaaact gggggtcggc gagtaagact cggcccaggg agcgcacggg cgtcgtgccg
     8041 caagtgggcc gcaggcggag atatcgggcc gcgtgcgcgg accgtgcaac acgcgcgaga
     8101 accgcaacgg atatgtaggc aatgatgccc acggcgttgg ccaaaatggg tcatggcgtc
     8161 ggcatggacc cctcctagta ttggtccatg ccggacgcgc gtacgcccgg gcccactcca
     8221 accatgcgac catgcggggg agcgaatgac gattgcaaac gatgccggca gcgtccggaa
     8281 gcgcgggatc gctagcggac cggtctggag acgacgcccg ttctttccga cagggagatt
     8341 cgacccggcg ggtgtcagga ctccgggact ttgaaaagcc ccctgtcagg gtacgcgccc
     8401 tcctcaagga ggtatctggg cgccgcggtc gccctgggtt cgatgtcagc cgccgtcacc
     8461 atcttccaga tggccttact ggcagggatc gtgtcccgcg tcttcctcga aggagctggc
     8521 attggcggtc tgacgacgtg gctggtgctt ctcgtgtgcg ctgtattctt gcgtgccggg
     8581 ctcgtgtggg cccgtgaagt ggtggccgaa cgcggcgcgg tgcgcgttaa ggccgagatc
     8641 cgtgagaggc tcttctccca tatcttgcgg ctgggaccgg cctacgccgc gggagagcgc
     8701 gctggcgagt tgaccacgac ggccacggag ggcgtggaga ggctggagcc ctacttcgcc
     8761 cactatctgc cgcaggcgtt gctcagcgtg ctcgttccgg tcatggtggc ggcgtacgtc
     8821 gccacgcaag accttacgag cgccgtgttg cttctggcga cggcccccgt gattcccgta
     8881 atgatggtcc tggtcggaag ttacgccgag gaacatacca ggcgacagtg gaccgcgctc
     8941 tcccggctcg gggcccactt cctggatgcc ttgcaggggc ttcccacgct gaaagctttc
     9001 ggacgggcca ggaacgaggc gcggcggctc gccgaaaccg gcgaggagtt ccgcgggcgg
     9061 acgatgaagg tgctcgggtt cgcgtttctg tccgggctgg tgctcgagtt catgacctcc
     9121 gccgccatcg cgctcctcgc cgtaatgctg ggggtgcgcc tgatcggggg cgccgtcgcc
     9181 ttcgagcagg cgtttctcgt gctgttgctt gcccccgagt tctaccgccc gctgcgcgag
     9241 ctcggcgtcc accgccacgc tggcatggag ggcaaagcgg cggccgaacg catccgcggg
     9301 atactccaaa cccccgcggg agctgacatc gtacccggcg attttcgtcc ctttcgcgcg
     9361 gtcgaggtag agatctcgga ggtcggtttc tcccaccccg agggctcgcg tcacgtactc
     9421 gacggcgtga gcatgacgct tccggtaggg agcatcacgg cgctcgtggg gcgcagcggt
     9481 gccggaaaga gcacgctggt caacctgctg ctccgctttc tggaacc
//
LOCUS       DBNGVD010000530         6430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2324422, whole genome shotgun sequence.
ACCESSION   DBNGVD010000530 DBNGVD010000000
VERSION     DBNGVD010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2324422"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="hemL"
                     /locus_tag="AC16CM_14330"
     CDS             complement(<1..1259)
                     /gene="hemL"
                     /locus_tag="AC16CM_14330"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3141058.1"
                     /translation="MDRSARLMERASGLIPGGVNSPVRAFRSVGGDPLFMERGQGSRV
                     FDVDGNPFIDYVMSYGPLVLGHAYPEVIEAVENTARKGTTFGAPTELEVELAETVCEA
                     VPSVEMVRMTNSGTEATMSAIRLARGYTGREKILKFDGNYHGHGDALLVAAGSGVATL
                     GLPDSPGVTRGAARDTSVLPYNDLEAVRDLFGREGGEFAAIIVEPVAGNMGCVPPAEG
                     YLEGLREITRSSGTVLIFDEVMTGFRLARGGAQERFGVTPDLTCLGKIIGGGLPVGAY
                     GGRREIMDLVAPSGPVYQAGTLSGNPLAMAAGLATLRVAGQPGFYDRLEELGARWRVG
                     MEAAASAGDRSYTVNQVGSMASIFFTEGPVTDFGSAAASDTKAFKDFFWHMLSRGVYL
                     APSQYEAGFISAAHSEADLQRTFEAAH"
     gene            complement(1413..1595)
                     /locus_tag="AC16CM_14335"
     CDS             complement(1413..1595)
                     /locus_tag="AC16CM_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141059.1"
                     /translation="MRYVLLIVGGLMDLVGVVWLLQGLGALPGSFMTGQPFWAVMGAI
                     FLVVGGLLVFAGLRSG"
     gene            complement(1818..2798)
                     /gene="hemB"
                     /locus_tag="AC16CM_14340"
                     /pseudo
     CDS             complement(1818..2798)
                     /gene="hemB"
                     /locus_tag="AC16CM_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
     gene            complement(2798..4333)
                     /gene="cobA"
                     /locus_tag="AC16CM_14345"
     CDS             complement(2798..4333)
                     /gene="cobA"
                     /locus_tag="AC16CM_14345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3141060.1"
                     /translation="MIYLVGSGPGDPGLFTVKGVRCMEEADVVVYDRLAPEALLKHAE
                     PGAEKIYVGKKPGNPTMSQEEINDLLVSLGHEGKTVVRLKGGDPYIFGRGGEEAIALL
                     EAGLPFEVVPGVTSGVAAPAYAGIPVTHRQVSTSVAFVTGHEDPTKGRTDVDWARIAN
                     GADTLVLYMGVGRLAEISAGLISAGKPPDTPVACVRWGTIPEQRTVTGTLENIADRVA
                     EAGLKPPAIIVVGDVVSLREAGLDWYERRPLFGRRVVVTRARAQAGELSVELERLGAE
                     VYEFPTIEISPPENFGPLDEAIRELDSFDWLVFTSVNGVEAFVGRLRRHGLDLRAVPR
                     GARVAAIGPATAERLEEAGLRVDVVPAEYRAEALIGALEAGSLAGRRVLIPRARVARE
                     ILPQKLEEAGAEVVVPPAYESVPSSAGKNELSLRLRNGEVDCVTFTASSTVENFVAAF
                     GGEEAALLLSATRVVCIGPITAGTARERGLRVDAEAAEYTIPGLVEAVTNLLAGGPAK
                     RSE"
     gene            complement(4330..4467)
                     /locus_tag="AC16CM_14350"
     CDS             complement(4330..4467)
                     /locus_tag="AC16CM_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141061.1"
                     /translation="MSRTFDGAVVRGILGSTRTGIEAVVSGPADAEQHGYSLLSPGVG
                     P"
     gene            complement(4468..5391)
                     /gene="hemC"
                     /locus_tag="AC16CM_14355"
     CDS             complement(4468..5391)
                     /gene="hemC"
                     /locus_tag="AC16CM_14355"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3141062.1"
                     /translation="MTPGHRAILGTRGSRLALVQAGGCAATLRAAGIEVEVRVIRTTS
                     EHLPDTPLSVIDQRDVFTRQLDEALLSGEIDLAVHSLKDVPTDLPEDVSLSAITRRHD
                     PSDVLVSEGRYTLDSLPHEAVVATSSLRRKAQLLHHRPDLKIVGIRGNVDTRVSKMRG
                     GAADAVVLARAGLERLGLDAPHTVIPPDVVLPAVGQGALAVAMRRDHPLLEPVREALN
                     HEPTEKAILAERAMLRTLEGGCRVPVGATSAVGGGVVSLRGLVISPDGALAYRGQAEG
                     GEPEEVGRRLALDLLEQGAAVVLGEVREVRR"
     gene            complement(5388..>6430)
                     /gene="hemA"
                     /locus_tag="AC16CM_14360"
     CDS             complement(5388..>6430)
                     /gene="hemA"
                     /locus_tag="AC16CM_14360"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3141063.1"
                     /translation="DTYWLTDAEAVHHLYRVASSIDSMIVGEGQILGQVREAYRMATV
                     EHCAGQILNRLFHTSLRIGKRVRSETGIGDSSLSVPRVAVKLAENVFGSLAGRRVLVL
                     GAGDMSELVVKQLKSRGVSELLIANRTAERARLLADRVGGRAVSFDAIDRELSGVDVV
                     VSSTGSGEWVLRSEAVAGATESREDPLFLIDIAVPRDVDPVVQTLENVYLYDIDDLQA
                     VVERNAEGRQEAAEEGEAMISPAVLEFTGWLSTLHVVPMIQELRDGAEQIRRHELARA
                     LKRMDLPPEEAAAVERMSHSLVNKLLHGPIQEIKARAEAGSPLESSEVRRTLLALDGL
                     DVELHRSRHRPG"
BASE COUNT         1051 a         2258 c         2068 g         1053 t
ORIGIN      
        1 tcgtgagctg cctcgaaggt cctctgaagg tccgcctcag agtgggctgc ggagataaag
       61 ccggcctcat actggctcgg ggccaggtag accccgcggg agagcatgtg ccagaagaag
      121 tccttgaacg ccttcgtgtc ggaagcggcg gcagatccaa agtccgttac ggggccctcg
      181 gtaaagaaga tgcttgccat ggagccgacc tggttgacgg tgtaggagcg gtccccagcc
      241 gaggcagccg cctccatacc cacgcgccag cgggcgccaa gttcctctag ccgatcatag
      301 aatccgggct ggccggccac acgcagggtc gcgaggcctg cggccatagc cagcgggttg
      361 cccgagaggg tgcctgcctg gtatacgggg ccggaagggg ctaccaggtc catgatctca
      421 cgcctcccac cgtaggcccc gacagggagg ccgccgccga tgatcttgcc caggcacgtc
      481 agatcgggcg tgacgccgaa gcgctcctgt gccccgccgc gggcgagcct gaagccggtc
      541 attacctcat cgaagatcag gacagtcccg cttgagcgcg tgatctccct cagtccttcc
      601 agatacccct ctgccggcgg gacgcacccc atgttcccag cgaccggctc cacgatgatg
      661 gcggcgaact ccccgccctc gcgcccgaag aggtcgcgca ccgcctcaag gtcgttgtag
      721 ggaagcacgg acgtatctct ggccgcccca cgtgtaacgc ctgggctgtc ggggagacca
      781 agggttgcca cgcccgaacc ggccgcgacg agcagggcgt caccatgccc gtggtagttg
      841 ccgtcgaact tgaggatctt ctcgcgacca gtgtatccgc gggcgagccg gatggcgctc
      901 atcgtcgcct ccgtccctga gttggtcatc ctgaccatct cgacggaggg aacggcctcg
      961 cacacggtct cggcgagctc gacctcgagc tccgtggggg caccgaaggt ggtgcccttc
     1021 ctggccgtgt tctctaccgc ctcgatcacc tcggggtagg cgtgcccgag gacgagcggc
     1081 ccgtagctca tcacgtagtc gatgaacggg ttgccatcca cgtcgaagac gcgcgaaccc
     1141 tgcccacgct ccatgaacag cgggtccccc cctaccgacc ggaacgcccg caccgggctg
     1201 ttcacaccac cggggatcag gccggaagcc ctctccatca acctcgccga acggtccatg
     1261 gcgagcagtg tcctcgcgtc gacgctctct ccactaccct gcaaacgcgc cagccccctt
     1321 cgctatacct ataccaagtc taatacggcc aacgccagac ggccgtgaca aagcttaccc
     1381 cagatagagg gacggcgggc tgctacgggg cgtcaaccag accgaagccc ggcgaagacc
     1441 agcaaaccac cgacgaccag gaagatcgcg cccattacgg cccagaacgg ctgcccggtc
     1501 atgaagcttc cgggcagggc gccgaggccc tgcagcaacc atacgacgcc gacgaggtcc
     1561 atcaatcctc ccacgatcag caacacgtaa cgcaattgat acctccgtct ggagcggttg
     1621 gcggtcatct tttcacgatt cccgtcaatt ttgcgagacc tctcagggca aggcaggtgg
     1681 aagcctggac ctcctccgcg cgcggggcgt gatccgcaaa ccgccctccg ccaaccagag
     1741 gagccggaat cccgaatctg tgtaggggcg cttcgcgcag cgcccgccga cggaccgtcc
     1801 ttacatgtaa cgtaccctca cccggacaac caacggaccg cgtcgggggc gtggtaggtg
     1861 atgatgatgt cggccccggc gcgctttatg ccggttagcg tctctatgac cgcccgctcc
     1921 tcgtcgagcc aaccgttctg ggccgccgcc ttgagcatcg cttactctcc gctcacattg
     1981 tacgccgcca ggggcaggtt ggttgtctca cggacccggc gcagcacgtc gaggtagggc
     2041 aacgcgggct tgaccataac gatgtctgcg ccctcctcga cgtcgaggcc ggtctccacg
     2101 agcgcttcgc gggcgttggc cgggtccatc tggtaactgc gccggtcgcc gaacttcgga
     2161 gcgctgtcgg cggcgtcgcg gaacgggccg aagaaggcgg aggcgtactt ggccgcgtag
     2221 gccattatgg gggtgttctc gaagccctcg ctatcgagct cggagcggat ggcggccaca
     2281 cgcccgtcca tcatatcgga gggggcgacg atatccgcgc cggcctcggc ctgcgaagcc
     2341 gcggtcctgg cgagcagctc caggctcacg tcgttcagga ccacgccggt ctccttctcg
     2401 acgacgccac aatgaccgtg gctcatgtac tcgcaaaggc acacgtcggt cacgacgagc
     2461 aggtctggcg cggcgtcctt tatggcgcgg gtggcgagct gaacgatgcc ctcggggtcg
     2521 taggccccgc tcgcggcctc gtccttgtgg tccgggatac caaagagcag gacagccggg
     2581 atgcctagct cgtgaactcg cacggcctcg gcaacggcgt ggcttatcga gagctgggag
     2641 atgccgggca tcgaggcaat ggggttcttc acgtcctccc cggccaccac gaagatcggg
     2701 tatacgaagt cctcgggcga gagctgcgtt tcgcgcacga ggccccgcaa gccggccgtg
     2761 cgcctggtcc ttctaagcct ttgttgggga aaagccatca ctcactcctc ttcgccgggc
     2821 cgcccgcgag gaggttcgtg accgcctcta cgaggcccgg aatggtgtat tcggcggctt
     2881 cggcgtcgac gcgcaggccg cgctcgcggg cggtgcccgc cgtgatcggg cctatacaga
     2941 ccacccgtgt cgcggagagc agcagcgccg cctcttctcc tccaaacgcc gccacgaagt
     3001 tctccaccgt tgagctcgcc gtgaacgtca cgcagtcaac ctccccgttt cgtagcctca
     3061 gggatagctc gttcttgccc gcagaagacg gtaccgactc gtaggcggga ggtacgacca
     3121 cctcggcgcc ggcctcctcg agcttctggg gtaggatctc cctcgcaacc ctcgccctgg
     3181 ggatgagcac gcgcctcccg gcgagcgagc cggcctcgag ggccccgatc agggcctcgg
     3241 ccctgtactc ggcggggacc acgtcgaccc tcagtccggc ctcctccagc ctctcggccg
     3301 tcgccggccc gatggcggcg accctcgcgc cgcgcggcac cgcccgcagg tctagcccgt
     3361 ggcgccgcag ccgccccacg aaggcctcca cgccgttcac gctggtaaag accagccagt
     3421 cgaaagagtc aagctcccgt atggcctcgt cgaggggtcc gaaattctcg gggggtgaga
     3481 tctcgatggt cgggaactcg tagacctcag cacctagccg ctccagctcg acggagagct
     3541 cgccggcctg ggcccgggcg cgggtgacaa ccacccttcg cccgaagagc ggccgcctct
     3601 cgtaccagtc caggccggcc tcgcgcaagg agaccacatc accgaccacg atgatcgccg
     3661 gcggcttcag gcctgcctcg gcgacgcggt ccgctatgtt ctccagcgtc cccgtgaccg
     3721 ttctctgctc cggtatggtc ccccacctga cgcaggccac gggtgtatcc ggcggctttc
     3781 cggcggagat aaggccggcg gagatctccg ccagccgtcc gacgcccatg tacagcacga
     3841 gcgtgtccgc cccgttcgcg atcctggccc agtccacgtc cgttcggccc ttggtcgggt
     3901 cttcgtggcc cgtgacgaag gcgacgctgg tcgagacctg cctgtgggtc acgggaatgc
     3961 cggcgtaggc gggggcggcg acgccgctcg tcacgccggg gaccacctcg aagggcagcc
     4021 cggcctccaa cagggcgatc gcctcctccc cgccacggcc gaagatgtac gggtcgccgc
     4081 ctttcaggcg cacgaccgtc ttgccctcgt ggccgaggga cacgaggagg tcgttgatct
     4141 cctcctgaga catcgtggga ttgccaggct tctttccgac gtagatcttc tcagcccccg
     4201 gctccgcatg cttcaggagc gcctccgggg cgagcctgtc gtagacgacg acgtcggctt
     4261 cctccatgca tctcacgccc ttgaccgtga acagtccggg atctcccggt ccgcttccga
     4321 cgaggtagat cacggtccca cccccggcga aagaagcgag tagccatgct gctcggcgtc
     4381 tgccggtccc gaaaccacgg cctctatgcc ggtcctggtc gagcccagga tgccacggac
     4441 tacggcaccg tcgaacgtcc gggacatcta ccgcctcacc tccctgacct cgccgaggac
     4501 cacagcagcg ccctgttcaa gcaggtccaa cgcgagccgc cttccgacct cctctggctc
     4561 cccgccctcg gcctgaccgc gataggcaag ggctccgtcg ggggagatta cgagccctcg
     4621 taggctgacc accccaccac caacggcgct ggtcgcaccc acagggaccc tgcagcctcc
     4681 ctccagcgtg cggagcatcg cccgctccgc gaggatcgcc ttctcggtag gctcgtggtt
     4741 gagggcctcg cgaaccggtt cgaggagcgg atggtctctc ctcatggcca cggccagggc
     4801 accctggccg acggcgggga gcacgacgtc cggcgggatc acggtgtgcg gggcgtctag
     4861 gccgaggcgt tcgaggccgg cgcgggccag caccaccgcg tcggcggcgc ccccgcgcat
     4921 cttgctcacg cgcgtgtcca cgttgcccct gatcccgacg atcttgaggt ccggcctgtg
     4981 gtgcaggagc tgggcctttc ggcgaaggct cgaggtggcg acgaccgcct cgtgcggcag
     5041 gctatccagg gtgtagcgtc cctcagagac gagcacgtcc gatgggtcgt gacgacgcgt
     5101 gatggcagag agcgaaacgt cctccggaag gtccgtggga acgtctttga gcgagtgcac
     5161 ggcgaggtct atctcccccg acagcagggc ctcgtcgagc tgtcgggtga acacgtcgcg
     5221 ctggtcgatc acagagagcg gggtgtcggg aaggtgctcg ctcgtcgtcc ttatgacgcg
     5281 cacctccact tcgatgccgg cggcgcgcag ggttgcggcg caccccccgg cctgcacgag
     5341 ggcgagcctg gagccacgcg tacccaggat ggcgcggtgg cccggggtca tccgggacgg
     5401 tggcgcgagc ggtgcagctc cacgtcgagg ccgtcaaggg cgaggagcgt gcgcctcact
     5461 tcggagctct ccagcgggga gccggcctcg gcgcgggcct tgatctcctg gataggaccg
     5521 tgcagcagct tgttgacgag cgagtggctc atacgctcga cggcggcggc ttcttcgggc
     5581 ggtaggtcca tcctcttcag ggcgcgcgca agctcgtggc gtcggatctg ctcggcccca
     5641 tcgcggagct cctggatcat gggcacgacg tgcagcgtgg agagccaacc ggtgaactcc
     5701 agcacggccg gggagatcat ggcctcccct tcttccgcgg cctcctggcg gccctccgcg
     5761 ttccgctcga ccacggcctg caggtcatcg atgtcgtaga ggtagacgtt ctccagcgtc
     5821 tggaccacgg ggtccacatc ccgcggcacg gcgatgtcta tgaggaacag cgggtcttcc
     5881 ctcgactcgg tggcaccggc tacggcctcg ctccggagca cccactcccc cgagccggtg
     5941 gagctgacca cgacgtcgac cccgctcagc tcacggtcta tggcgtcgaa cgagacggcc
     6001 ctgcccccca ctcggtccgc gagcaacctg gccctttcag cagtacggtt ggcgatgagg
     6061 agctcggaga cccccctgct cttgagttgc ttgacgacga gctcgctcat gtctccggca
     6121 ccaagcacca ggacccgcct cccggcgagc gagccgaaga cgttctcggc gagcttgaca
     6181 gccacacgcg gcacggagag agagctatcg cctatcccgg tctccgagcg gacccgtttg
     6241 ccaatcctca gcgaggtgtg gaagaggcgg ttgaggatct ggccggcgca atgctccacg
     6301 gtcgccatcc tgtaagcctc gcggacctgg ccgaggatct gcccctcgcc cacgatcatg
     6361 gaatcgatcg atgacgccac cctgtagagg tggtgaacgg cttcggcatc ggtcagccag
     6421 taggtatccc
//
LOCUS       DBNGVD010000531         3689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2870388, whole genome shotgun sequence.
ACCESSION   DBNGVD010000531 DBNGVD010000000
VERSION     DBNGVD010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2870388"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /gene="mbhE"
                     /locus_tag="AC16CM_14365"
     CDS             <1..1386
                     /gene="mbhE"
                     /locus_tag="AC16CM_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HIR3141064.1"
                     /translation="VSLGASTPLDASYHPEILPEYVMALAAYAVGVGLILSRSIWSPA
                     ALGVSRLGKLAGPERLFGMTVRGLNRLSDAVHRVEIQNLRGRVASVLVPTAVMVGAGL
                     LATGLGSYDLGPLRLREAPVMLALLAVAAASIAATLTRRHVTLALVLSSVGFMLAVVY
                     AFYGAPNVALVAVLIETVQTLLLVATLRLLPYRVLRHQAELPLKRRNRKVLVAAASGA
                     FGFVVVWGALSQPPAGQSVAEELTRLTPEAHAKNVVTATLADFRGLDTLGEITVVALV
                     LLGVATLLGGLAPERNGGPDGGRSAAIPAARVVVRGIARLLYLPTFLVAAALLVKGFV
                     ETGDGFSAGVVAALGVLLRYLAFGYERTKGLPAVRHSLAVAFCGLLLALGVAAAPLLF
                     GEAPLTHYPPAGTEPIHVGTIELMTAVLFDVGVFVLVFGFTVGVVSIFARAGEAVGQD
                     GYPDGRGAERS"
     gene            1383..1805
                     /locus_tag="AC16CM_14370"
     CDS             1383..1805
                     /locus_tag="AC16CM_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR3141065.1"
                     /translation="MTLYAALVIAIVLASGTFLLLQRDLFRVVIGIILVSNSATLFIV
                     ASGLSRGAAPIYPLPDSGLSDPLVQAMALTALIIGFGVAALLFAMVYQLYASHHTMDL
                     EEIAEAEAREAEDLDLRGDPGTEEVPEEEQDPEERTVP"
     gene            1802..3202
                     /locus_tag="AC16CM_14375"
     CDS             1802..3202
                     /locus_tag="AC16CM_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HIR3141066.1"
                     /translation="MTEPGLLLALPVGIPWVLAAVLPLLDGRKRWVGLLAAAGLGASL
                     LSLVWLASIVIRRGEVEVTAGGWPEGVGITLRADALGVTFALVSVAVILAAFLYEVAL
                     GVRWRAFPALILLVAAGLTGLFFTGDAFNFYVFFEISMISSFILAGYREKDYQVRAAF
                     VFAVVNLLGSFVFLIGIAALYTISGSLDMETIAARVAGLNPSSVIAISALIFAAFFLK
                     LGLFPFHFWLPAVYVGSHPPIAAILSGALANIGAYGLLRFGADVLPRELALAAPAVIV
                     IGAASIAYGSLQAVSRRDTSEVMAYSAIGQAGYVLLALGVGGPVGYAAAVVYAVVNSL
                     NKGVVFLSAGLRGPLVGAAFAVGAFSIAGVPPAAGYLGKFMVFEASTAVESVTATVAL
                     VFLVFVGRALSFLYSFQVYQRRFLAPGASGKPSPAAARYLVVLLAALVVLLGLWPQPL
                     MLLSRAAAAALTGSPG"
     gene            3199..>3689
                     /locus_tag="AC16CM_14380"
     CDS             3199..>3689
                     /locus_tag="AC16CM_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR3141067.1"
                     /translation="MTRYVAAVVLLTLVYGLALASFDPLDLLFGAGLSAVLVYLSRRF
                     VFGEDPGGGPSLLGRAVAFVPFAFAVLKEILKGTWQVTLVTLHLSPLDEPGIIAVPIG
                     ERTPTGVSAWAVVTGLPPGSFFVDIDREREVALIHVIDASDPDAFREEQADFYRRYQS
                     KVFP"
BASE COUNT          422 a         1275 c         1266 g          726 t
ORIGIN      
        1 gtctctctgg gagcatcgac gccccttgac gcctcctacc accccgagat cctccccgag
       61 tatgtgatgg cgctggcggc ttacgccgtg ggcgtgggcc tgatcctctc ccgttccatc
      121 tggtctccgg cggccctcgg cgtctcccgc ctcggcaagc tcgcagggcc cgagcggctg
      181 ttcgggatga ccgtcagggg cctcaaccgt ctctcggacg ccgtccaccg ggtggagata
      241 cagaacctca ggggacgggt cgcgtccgtg ctcgttccga cggcggtgat ggttggcgcg
      301 ggcctgctcg cgaccggcct cggctcgtac gatctgggtc ccttgcggct tcgggaggcg
      361 ccggtcatgc tggccctcct tgccgtcgcg gcggcctcga tcgcggccac gctcacgcgg
      421 cggcacgtca cgctcgcgct ggtcctgtcg agcgtgggct tcatgctcgc ggtcgtctac
      481 gcgttctacg gggcgccgaa cgtggccctc gttgccgtcc tgatcgagac cgtgcagacg
      541 ctgctgctcg tcgccaccct gaggctgctc ccctaccggg tcctgcgcca ccaggcggag
      601 cttcctctca agaggcgcaa ccgaaaggtg ctcgtggcgg cggcctccgg ggccttcggg
      661 ttcgtggtgg tctggggtgc cctctcgcag ccgccggccg gtcagagcgt ggctgaggag
      721 ttgacccggc tcacgccgga ggcgcatgcc aagaacgtcg tgacggccac gctggcggac
      781 ttccgtggcc tggacaccct gggggagatc accgtggtgg ccctggtcct cctgggggtg
      841 gcgaccctgc tcggggggct tgcacccgag cgtaacggcg gaccggacgg cgggcgtagc
      901 gccgcgatac cggctgcgag ggtcgtggta cgcggcatcg caaggctgct ctacctgccg
      961 acgttcctag tcgccgcggc cctgctggtc aagggcttcg tcgagaccgg cgatgggttc
     1021 tccgccgggg tggtagcggc gctcggggtg ctgctccgct acctggcctt cggttacgag
     1081 aggacgaagg gtctaccggc cgtgcgccac tccctcgccg tcgcgttctg cgggctcctc
     1141 ctggcgcttg gcgtggccgc tgcgccactg ctcttcgggg aagcgcccct gacccactat
     1201 ccccccgccg gcaccgagcc catccacgtg gggaccatcg agctgatgac cgccgtactg
     1261 ttcgacgtcg gggtcttcgt gctggtcttc ggtttcacgg ttggggtggt gtccatcttc
     1321 gcccgcgccg gcgaagccgt cgggcaggat ggttatcctg acgggagggg ggcggagaga
     1381 agttgaccct gtacgccgcg ctcgtgatcg ccatcgtgct cgcctctggt acgttcctgc
     1441 ttctccagcg cgacctcttc cgggtggtca tcggcatcat cctcgtctcc aactcggcga
     1501 ccttgtttat cgtcgcctcc gggctgtcga ggggcgcggc gccgatctac ccgctccccg
     1561 acagcggctt gagcgacccg ctggtacagg cgatggccct gaccgccctc atcatagggt
     1621 ttggcgtcgc cgcgctgctc ttcgccatgg tctaccagct gtacgcctcg caccacacca
     1681 tggacctgga ggagatcgcc gaggcggagg ctcgcgaggc cgaggacctg gaccttcggg
     1741 gggatcctgg cacggaagag gtacccgagg aagagcagga cccggaagag aggacggtgc
     1801 cgtgacggaa cccggcctcc tcctcgccct cccggtcggc atcccgtggg tgctcgcggc
     1861 cgtgctaccc ctcctcgacg gccgcaagcg gtgggtcggc ctgctggctg ctgctggcct
     1921 cggggccagc ctcctctccc tcgtctggct cgcctccata gtcatcaggc ggggagaggt
     1981 cgaggtgacc gcgggcggct ggcccgaggg cgtcggcatc acgctcaggg ccgacgccct
     2041 cggggtgacg ttcgccctcg tctccgtcgc ggttatcctg gccgcctttc tctacgaggt
     2101 ggcactcggg gtgcgctggc gggcgttccc ggccctgatc ctgctggtgg ccgctggtct
     2161 cacgggcctg ttctttacgg gcgatgcctt caacttctac gtgttcttcg agatctccat
     2221 gatctcctcc ttcatcctgg caggctacag ggagaaggac taccaggtga gggcggcgtt
     2281 cgtcttcgcc gtggtcaacc tgcttggctc cttcgtcttc ctgatcggga tagcggcgct
     2341 ctacacaatc tcaggcagcc tggacatgga gaccatcgcc gccagggtgg ctggcctgaa
     2401 tccctcctcg gtgatcgcca tatccgccct gatcttcgcg gcttttttct tgaagctggg
     2461 gctctttcct ttccacttct ggctgccggc ggtctacgtg ggaagccatc ccccgatcgc
     2521 cgccatcctc tcgggggccc tggccaacat cggggcctac ggcctgctgc gcttcggcgc
     2581 ggacgtgctg ccgcgagagc tcgcgctcgc ggcgccggcg gtgatcgtga tcggggccgc
     2641 cagcattgcc tatggctcgc tgcaggccgt gtcccggcgc gacacctcgg aggtgatggc
     2701 ctactcggcg atcgggcagg ccgggtacgt gctcctggcc cttggcgttg gcggcccggt
     2761 ggggtatgcg gcggccgtgg tgtacgcggt cgtcaactcc cttaacaagg gcgtggtctt
     2821 cctctcggcg gggctgcgcg ggccgctggt cggcgcggct ttcgcggtcg gggccttctc
     2881 gatcgcgggc gtgcccccgg ccgccggtta tctgggcaag ttcatggtgt tcgaggcgag
     2941 caccgccgta gagagcgtta cggcgaccgt tgccctcgtc ttcctggtgt tcgtcggtcg
     3001 cgcgctctcc ttcctctact ccttccaggt ctaccagcgc cgcttcttgg cgccgggggc
     3061 gtctggcaag ccgagcccgg cggcggcgcg ctacctggtg gttctcctgg cggccctcgt
     3121 agtgctgctc gggctgtggc cccagcccct catgctcctg agcagggcgg cggccgccgc
     3181 gctgacgggg agcccgggat gacgcgctac gtggcggcgg tcgtcctcct gacgctcgtg
     3241 tacgggctcg ccctggccag cttcgatccc ctggatctgc tcttcggagc cgggctctcg
     3301 gcggtgctgg tctatctgtc ccgccggttc gtcttcggcg aggaccccgg cggcgggccg
     3361 tccctgctcg gaagggcggt ggccttcgtg ccttttgcgt tcgccgtcct caaggagata
     3421 ctaaagggta cctggcaggt caccctggtc acgctccacc tcagccccct ggacgagcca
     3481 ggcatcatcg ctgtgcccat aggcgagagg acgccgacag gggtctccgc ctgggccgtg
     3541 gtcaccggcc tgccgcccgg ctcgttcttc gtggatatcg accgggagag ggaggtggcc
     3601 ctgatccacg ttatagacgc cagcgatccc gacgcctttc gagaggagca ggcagacttc
     3661 taccgccgct accagagcaa ggtgttccc
//
LOCUS       DBNGVD010000532         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2325520, whole genome shotgun sequence.
ACCESSION   DBNGVD010000532 DBNGVD010000000
VERSION     DBNGVD010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2325520"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1838
                     /locus_tag="AC16CM_14385"
     CDS             345..1838
                     /locus_tag="AC16CM_14385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3141068.1"
                     /translation="MRPVIREVFALRRLRWKLTLSYTLVAAVTLLALELILSGGLVAF
                     LNSDLPPRLIAQNLRDEVAPRLEQPMRQDPPDAQAIRRRLDVFASTSGVRGIDRPPPP
                     PTSIGDYAAYGGALFVVDEERELLVSLPTVRGLPEGEQFDASKVQGLEPLVEAGLGGE
                     ENSGELSANVNGQVLTVTPIEGKKGKVAGVLVGTLRLPNLTAPLLITVGISAIFLAVP
                     AALLGALFGFFTAWGLTRRIGRLVRASRSWSRGDFSVTTRDRSRDELGQLSRELNSMA
                     AELENLLQTRGELATLEARNQFARDLHDSVKQQVFATSLQIAAARAMIEKDTGSAESH
                     LSQADELVRQAQKELNVLIQEMRPAALEGKGLATALGEYVTRWSDGAEIPIDFRVQGE
                     REVPLEAEQALFRIAQEALANVARHSEAGQVEVDLLYTRDTVTLRVSDDGRGFDPSRN
                     PGGGFGLQSMRERLVRLGGHVNVESAPGKGTRVEGFCPLWREKGNES"
     gene            1835..2488
                     /locus_tag="AC16CM_14390"
     CDS             1835..2488
                     /locus_tag="AC16CM_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3141069.1"
                     /translation="MSEPITVLLVDDHALVRQGVRAFLETQGDISVVAEAGSGEEAVR
                     LAAEHAPDVALMDLIMPGMDGVEATRQLVAISPRTSVVMLTSYHDDEHVFPAIRAGAL
                     SYVLKEIGPTELADAVRKAASGEAVLHPRVAARVVRELHGARRTEPNVFQELSDRELE
                     VLKLIAEGLHNSEIAARLYVSEKTVKSHVSNILSKLHLADRTQAAVYAWREGVVRRE"
     gene            complement(2552..>3189)
                     /locus_tag="AC16CM_14395"
     CDS             complement(2552..>3189)
                     /locus_tag="AC16CM_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3141070.1"
                     /translation="GACARISRRARKRDIVAKSSARVHRSGGSDGSRKVYLIVGTIIV
                     LFVGGFIALAVFDARQQSASGAPEDVQTYDVGPAGEHTSTNVDYEQTPSVGGAHDPVW
                     QNCGFYGQPVREENAVHSLEHGAVWIAYSPDLPQDQVTELQNLAQSQTYILASPYPDL
                     ESPVVASAWGKQVALDGADDPDLQSFIKAYRLGPQTPEPGAVCTGGTGATL"
BASE COUNT          563 a         1036 c         1036 g          554 t
ORIGIN      
        1 tggggatagt ggactcgttc gccgcgcaga tctccggcat cagctacacg gtgccgtggg
       61 gcacccttgg cgtgatcgtg ggcctcgcct acctcgcctc cctgctcacc accttcctcc
      121 ccgcccggca ggcctcgaag gtttaccccg ccgaagcact ccggtacgag taaggctaca
      181 ggctacaggc ttcagaaata tcacctgtag cctgtagcct gtaatctccc ccgttcacta
      241 tatctgcgaa cgggctctag tccccgagta tgatccccat tacgtctacg ggccgatgcg
      301 gcccttctct cacggtacta aacttcccgt cgtatgggag acaagtgcgt ccggtgatcc
      361 gcgaggtgtt cgcgctccgg cggctacggt ggaagctgac cctcagctac acgctggttg
      421 ccgcggtaac gctgctcgcg ctcgagttga tcctttccgg tggcctggtc gcctttctca
      481 actccgacct tcctccgcgc ctgatcgcac agaacctcag ggacgaggta gccccccggc
      541 ttgagcaacc gatgaggcag gacccacccg atgcacaggc cataaggcgg aggctagatg
      601 tgttcgccag cacttcgggt gttcgcggga tagacaggcc gccaccgcca cccaccagca
      661 tcggcgacta cgccgcctac ggcggcgcgc tcttcgtggt cgacgaggag cgagaacttc
      721 tggtctcact ccccacggtg cggggcctcc ccgaaggcga gcagttcgat gcctcgaagg
      781 tgcagggcct cgagccgctc gtggaggcgg ggctcggcgg cgaggagaac tcgggagagc
      841 tctccgccaa cgtcaacggc caggtgttga ccgttacccc catagagggc aagaagggca
      901 aggtcgccgg ggtgctggtc ggcacgctgc gtttgcctaa cctcacggcc ccgctcctca
      961 taaccgttgg tatcagcgcg atcttcctcg ccgtgccggc ggcgttgctc ggcgcgcttt
     1021 tcgggttctt taccgcctgg ggtctgaccc ggcgtatcgg tcgcctcgta cgggcctccc
     1081 gctcgtggag caggggagac ttctccgtta cgaccaggga ccgctccagg gacgagctcg
     1141 gccagctctc gcgcgagctg aacagcatgg ccgccgagct cgaaaatctc ctccagacca
     1201 ggggtgagct cgctacgctc gaggcccgaa accagttcgc ccgcgacctt cacgactccg
     1261 tcaaacagca ggtcttcgcg acctcgctcc agatcgccgc ggcccgggcc atgatagaga
     1321 aggacacagg ctcggccgag tcacacctct cccaggccga cgagctcgtg cgtcaggccc
     1381 agaaagagct gaacgtcctg atccaggaga tgcggccagc cgccctggaa gggaagggcc
     1441 tcgccacggc gcttggtgag tacgtcaccc gctggtccga cggggccgag atccccatcg
     1501 atttccgcgt gcagggcgag cgtgaggttc ccctcgaagc cgaacaggcc ctcttccgca
     1561 tcgcccagga ggcactggcc aacgttgcca ggcacagcga agccgggcag gtagaggttg
     1621 acctcttata caccagagat accgtaacgc tgcgggtctc cgacgatggc cgcggcttcg
     1681 atccttcccg caacccaggt ggggggttcg gtcttcagag catgcgcgag cggctggtca
     1741 ggctcggcgg gcacgtcaac gtggagagcg caccgggcaa aggaacgcgg gtagagggct
     1801 tctgcccatt atggagagag aaaggcaacg aatcatgagc gagccgatca cggtacttct
     1861 ggtagacgat catgctctgg tgcgacaggg ggtgcgagcc tttcttgaaa cgcagggtga
     1921 tatcagcgtg gtcgccgagg ccggcagcgg ggaggaggcg gttaggctcg ccgcggagca
     1981 cgcccccgac gtcgccctga tggatctcat catgccgggc atggacggtg tcgaggcgac
     2041 acggcaactc gtcgcgatct cgcctcgcac gagcgtggta atgctgacct cctaccacga
     2101 cgacgagcac gtcttcccgg cgatccgggc cggggcccta tcctacgtct tgaaggagat
     2161 cggacccaca gagctcgccg acgccgtgcg gaaggcggca tctggcgagg ccgtgctcca
     2221 tccacgggtc gcggcccggg tcgtgcgcga gctccacggc gccaggcgca ccgagccaaa
     2281 cgtcttccag gagctctcgg acagggaact cgaggtcctg aagctgatcg ccgaagggct
     2341 gcacaactcg gagattgcgg cccgcctcta cgtgagcgag aagaccgtca agagccacgt
     2401 ctcgaatatc ctctccaaac tccacctcgc cgaccggacc caggcagccg tctacgcctg
     2461 gagagagggc gtagtccgcc gcgaataaag tggttcgctt ctcgaaccgc gctgtcagct
     2521 atcagcagtc agcaagagaa gctgatagct attacaacgt tgcgccggtg ccgccggtac
     2581 agacggcgcc gggttcgggg gtctgggggc cgaggcggta ggctttgatg aagctctgaa
     2641 ggtcgggatc atcggcgccg tcgagggcga cctgcttgcc ccaggccgag gctaccacgg
     2701 gggactcgag gtcggggtag gggcttgcga ggatgtaggt ctggctctgg gcgaggtttt
     2761 gcagttccgt tacctgatcc tgcggcaggt cgggggagta ggcgatccag accgcaccgt
     2821 gctccagaga gtgaacggcg ttctcttcgc ggacgggctg gccgtagaag ccgcagttct
     2881 gccagacggg gtcatgcgcg cccccgaccg aaggggtctg ctcgtagtcc acgttggttg
     2941 aggtgtgctc gcccgcgggg ccgacatcat aggtctgcac gtcctcgggc gcgccgctgg
     3001 cggactgctg cctggcgtcg aacacggcga gcgcgatgaa cccgccgacg aagaggacga
     3061 tgatcgtgcc aacgatgagg tagaccttgc gggagccatc cgacccgcca ctcctatgaa
     3121 cccttgcgct gctcttggcc acgatgtccc tctttctagc tcgccgtgaa atcctagcac
     3181 atgcgcccg
//
LOCUS       DBNGVD010000533         2725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2143592, whole genome shotgun sequence.
ACCESSION   DBNGVD010000533 DBNGVD010000000
VERSION     DBNGVD010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2143592"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="AC16CM_14400"
     CDS             <1..1271
                     /locus_tag="AC16CM_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR3141071.1"
                     /translation="PEQCATEVFFIPSALVAEKDGSFTQTQRMLQWHDKAVEPPGDAR
                     SDAWFVYHLGRRIKELYEDSEAPRDRLIQAMTWDYPTEGEREEPHIESILKEISGYTV
                     ADGEPVSGFADLEDDGTTACGGWIYSGSYADGINQPRRRKPHTEQPPAANEWGWAWPA
                     DRRILYNRASADPEGKPWSERKRYVWWDEEQGKWVGHDIPDFTATKRPDYRPEPDATG
                     DDAIPGDGPFILNGDGLGWLFAPVGLADGPLPVHYEPVESPVPNLLYDNVQYTPAVKL
                     LDRADNPYNPPQDPRFPHVVTTYRLTEHHTSGAMSRWIPWLSELQPELFCEISPELAA
                     EKGVENTGWVTISTSRGKIHCKALVSDRIPVYDFEGRKVHTIGLPYHWGPNGLVKGDV
                     VNDLMPVSLEANVAIHEAKAFTANLEAGRV"
     gene            1276..1608
                     /locus_tag="AC16CM_14405"
     CDS             1276..1608
                     /locus_tag="AC16CM_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141072.1"
                     /translation="MRDPRGTRTTPEDTTSDTQRAESSHRETMQQDLMDLGAWLSEHP
                     GAAPKNLKPIRSAKVLDPGVYYNMGTGMVDRIFSPQRVALGHKMFRVSRDPAAPVEEI
                     RHRVLEGK"
     gene            1612..2454
                     /locus_tag="AC16CM_14410"
     CDS             1612..2454
                     /locus_tag="AC16CM_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3141073.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELTGDSYDNTR
                     QLSGTTWRHVAFIENPRKRRLTGDVDLVALAEDVTEPLPGGAELDALGRWAFMSDVCK
                     HCVAAPCQQACPTGSIIRNEFDNVYIQPDICNGCGYCVPACPYGVITQVPNDGRAFKC
                     TLCYDRQRDGLEPACAKACPTESIQFGEVEDLKARAEARVQELHDKGYTQAYVWGTPE
                     VGGTGGIGGNHSKFILMDEPETYNLPRHPFLPQEKVKGGTIASMVAGAVLGAVVALAF
                     RERG"
     gene            2458..>2725
                     /locus_tag="AC16CM_14415"
     CDS             2458..>2725
                     /locus_tag="AC16CM_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141074.1"
                     /translation="MGETGRDRIQQGNGRAETTHHPETDAKRKDKNYYGIPPIKRAHW
                     TWQIPLYFWIGGIGAGVQLFSTMAQILGHEDQALTRASRYTVLGT"
BASE COUNT          555 a          931 c          873 g          366 t
ORIGIN      
        1 accccgagca gtgcgccacg gaggtcttct tcatcccctc ggcgctcgtc gccgagaagg
       61 acggctcctt cacccagacc cagcgcatgc tgcagtggca cgacaaggcc gtcgaaccgc
      121 cgggcgacgc caggagcgac gcctggttcg tctaccacct ggggcgcagg atcaaggagc
      181 tctacgagga ctccgaagcg ccacgcgaca ggctcataca ggccatgacc tgggactacc
      241 cgaccgaggg cgagcgcgag gagccgcaca tagagagcat cctcaaggag atcagcggct
      301 acaccgtagc agacggggag ccggtctcgg ggtttgccga cctcgaggac gacggcacca
      361 cggcgtgcgg tggttggatc tactccggct cctacgccga tggcatcaac caaccaagga
      421 ggcgcaagcc ccacaccgag cagccgcccg cggccaacga gtggggctgg gcctggccgg
      481 ctgaccgtcg catcctctac aacagggcct ccgccgaccc cgagggcaaa ccgtggtccg
      541 agcgaaaaag gtacgtctgg tgggacgagg agcagggcaa gtgggtcggc cacgacatcc
      601 ccgacttcac ggcgaccaag cgtcccgact acaggcccga acccgacgcc acgggcgacg
      661 acgccatacc aggggatggg ccgttcatcc tcaacggcga cgggctcggc tggctcttcg
      721 cccccgtggg gctcgccgac ggcccactcc ccgttcacta cgagcccgtg gagtcgccgg
      781 tcccaaacct tctctacgac aacgtgcagt acaccccggc ggtcaagctc cttgaccggg
      841 cggacaaccc gtacaacccg ccgcaggatc cgaggttccc ccacgtagtt accacgtatc
      901 ggcttacgga gcaccacacc tcgggtgcca tgagccgttg gatcccctgg ctctccgagc
      961 tccagcccga gcttttctgt gagatcagcc ccgagctggc cgccgaaaag ggcgtggaga
     1021 acaccggctg ggtcaccatc tcgacgagcc ggggcaagat ccactgcaag gccctcgtct
     1081 ccgaccgcat ccccgtctac gacttcgaag gcaggaaggt gcataccatt ggcctcccct
     1141 accactgggg accaaacggc ctcgtcaagg gcgacgtcgt aaacgacctg atgccggtct
     1201 ccctggaggc aaacgtcgcc atccacgagg caaaggcatt caccgccaac ctcgaagcgg
     1261 gaagggtata gcgccgtgag ggatccaagg ggcacgcgca ccacccccga ggacaccacc
     1321 agcgataccc agagggccga gagctcccac cgggagacca tgcagcagga ccttatggac
     1381 ctcggggcgt ggctgtccga gcatccgggg gcggcaccga agaacctcaa gcccataagg
     1441 agcgcgaagg ttctggaccc gggcgtctac tacaacatgg gcacggggat ggtggacagg
     1501 atcttcagcc cccagcgcgt cgccctcggc cacaagatgt tccgcgtaag cagagacccc
     1561 gccgcgccgg tggaggagat ccggcaccga gtgctggaag ggaagtgacg gatggccacg
     1621 ggattcctta ccgacaccac ggtctgcata ggctgcaagg cgtgcgaggt cgcctgcaaa
     1681 cagtggaacc agctccccgc cgacggctac gagctgaccg gcgactctta tgacaacacc
     1741 aggcagctct ctggaactac ctggcgccac gtcgccttca tcgagaaccc caggaagcgg
     1801 cgcctgaccg gggacgtaga cctcgtagcc ctggccgagg acgttacgga gccgctgccg
     1861 ggtggcgccg agctcgacgc cctcgggcgt tgggccttca tgagcgacgt gtgcaagcac
     1921 tgcgtggcgg cgccgtgcca gcaggcctgc ccgaccggct cgataatccg caacgagttc
     1981 gacaacgtct acatccaacc cgatatctgc aacggctgcg gctactgcgt ccccgcctgc
     2041 ccctacggcg ttatcaccca ggtgcccaac gacggccgcg ccttcaagtg caccctctgc
     2101 tacgaccgcc agagggacgg cctcgaaccg gcctgcgcca aggcctgccc cacggagtcc
     2161 atccagttcg gcgaggtcga ggacctgaag gcccgcgccg aagcgcgggt acaggaacta
     2221 cacgacaagg gctacacgca ggcctacgtg tgggggacgc ccgaggtcgg gggtacgggc
     2281 ggcatagggg gcaaccactc caagttcatc ctcatggacg agccggagac ctacaaccta
     2341 cccaggcatc ccttcttgcc gcaggagaag gtcaagggag ggaccatcgc gagcatggta
     2401 gccggggcgg tgctcggggc ggtcgtggcg ctggcgttca gggagcgggg gtagagaatg
     2461 ggcgagacgg ggagggaccg gatccagcag ggcaacgggc gggcggagac aacgcaccat
     2521 cccgagacgg acgcgaagcg taaggacaag aactactacg gcatcccacc cattaagcgg
     2581 gcgcactgga cgtggcagat cccactgtac ttctggatcg ggggtatcgg agctggggtc
     2641 cagctgttct ccacaatggc ccagatcctc ggccacgaag accaggccct cacccgcgcc
     2701 agccgctaca cggtgctcgg cacca
//
LOCUS       DBNGVD010000534         5935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4695229, whole genome shotgun sequence.
ACCESSION   DBNGVD010000534 DBNGVD010000000
VERSION     DBNGVD010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4695229"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="AC16CM_14420"
     CDS             complement(<1..936)
                     /locus_tag="AC16CM_14420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3141075.1"
                     /translation="MNEGVAGPTKFQVLATKGIPTFLLLEGAADDEVRSSHRDDEDER
                     LGALAMGIEGSLPPPEVRPWPGGFAVTLAELAAEDFFPRALDTAGPASPDHALYRALA
                     GGRYVRAVNFHATPERQAGQLEEQLSRLAEAFAPVSYEDLARLVQTGDWHHDRPGVIL
                     NFFDGFRDNFGVVAPILDRLNMVGWFFVVSGWISAPPEDQRSFAWRHAIDLPYHGEDL
                     PPDGRLALAPEELRDLAERGHVVVSHTRTHVSASPGAVPGLRPVTLEREASASKQDLE
                     EIVGRPVGALAWLEGAALGASGAADRAVARAGYSLL"
     gene            complement(936..2450)
                     /locus_tag="AC16CM_14425"
     CDS             complement(936..2450)
                     /locus_tag="AC16CM_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HIR3141076.1"
                     /translation="MPEPVPLFEPPQRTVSEPPEVAAYRRALPDGEAFAYPLDPLDHL
                     GLPVWSAELFTARGTAHHGAGYGESDERAVTGAFGELSETLFAADAVRAMTPRRASYR
                     DLVPEVGREGVIDPLTVCLPAGSGYSPERPLCWVLARRWTTGEPVYVPLELAAASSVD
                     IDWEGELLVQPVTNGLGAGPTVEHAMAHGLLELVQRDGNSVAYRALDQGVVISGLEAV
                     DKPETRALLEKLDRHGIEVVVKLASTDFGINNIYVVGADRDPVRAPHPLALSACGEAA
                     DPDREKALSKALMEFCAGRARKPFDNGPLSRMTAVAPATYVGRAIRAATLESEEERAT
                     RGAVDWLGLDAYEMRALLEDPVFAARSEVDFSSLPTLPGGAEDSNSPANLVANRLRED
                     GLDPLYVDLSPPGGEVRVVRAIVPGLEVETASYARIGARNLRRLLCRDDDLVGVDEPP
                     PGAKPILLPGQAREEFGPAPWLNVDLLEKRVGRLYPLYREPSRHVAGLVASGVL"
     gene            complement(2454..3296)
                     /locus_tag="AC16CM_14430"
     CDS             complement(2454..3296)
                     /locus_tag="AC16CM_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3141077.1"
                     /translation="MKGTRRTTSEPVKLLAYVFLGVGALVTLYPFYWMVTASTLTESE
                     IFRAPPRLLAGSHFLTNLDALSQAAPFLRAMLNSIVVAGLTTVSTVLLSALAGYAFAK
                     FAFRGRKLLFAVVLGTLMLPTEIMIVPLFIIMLQLGWVDNFAALIVPYLVTAFGVFLM
                     RQQMMGFPNDLLDAARIDGNGELGVFFRVVLPNMKAACAALGIVTFMSQWGNFLWPLV
                     VMSTPERFTYPLMLASLVRQGGAVPYGPVMVAAVVGLVPLILLFFFFQRFIISGVFSG
                     SLKE"
     gene            complement(3298..4194)
                     /locus_tag="AC16CM_14435"
     CDS             complement(3298..4194)
                     /locus_tag="AC16CM_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3141078.1"
                     /translation="MTVSRKSRTRAGWSTWAPYAFIAPFFLVFAAFILYPILYSAALS
                     FGQFSGGRIIFTGLDNYRRLLADGLFLKSLLNTGLILVVQVPLMIGLATIIASVIDSD
                     FLGRRRKAFFRLAFFLPVAIDLVTYSVVFSLIFSEQYGIVNQALGLVGLDGIPWRSDP
                     FWARVLIILALTWRWTGYNAVILLSGLQNIPKDLYDAASVDGAGSVVRFLRITVPLLR
                     PVILFCTVLGTIGTLQLFTEPYILTSGGPANATLTSFYYIYDTGFARFDFGLAAAGTY
                     VLSAIVAIMSYAQLRLARWGEV"
     gene            complement(4199..5530)
                     /locus_tag="AC16CM_14440"
     CDS             complement(4199..5530)
                     /locus_tag="AC16CM_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3141079.1"
                     /translation="MGDTRHTGAGLDMTGGMSRRRFLKLGGVGLAGAALLGGGPLVGC
                     GSSDSPGLTVASWDVARDALKNTVPLFKKKRPDTPVRMQSITIDYQQIIPRLQAGSGA
                     PDIFSLGQQDFQNFLERFPGQFVNVTDRLAEYEGQFAEAPLEHARKDGKLYGVPWDMG
                     PVGMWYRKDLFEQAGIGPDDVATYDDFVAAGQELERKTGAKMTAFDASGGGTNSSYYQ
                     LLLNQQSGGFYSDGGKIDFSNEKSYSAMEVLDGFVNENIAVDTPTYDELSRAISNDDT
                     ATDLGAAWDVGLIKGAGGKSQKGKWDVLPLPAFDSGGPQEATLSGSVLVISAQSQDVD
                     AAWDFIHEALLTKEGQSKSWAQGLFPSWEPYWDTAAFNEPDPYFGFAIAPKFADIARN
                     VQALEYGPHFLDFQKPLMDAYASILTTDTTPQEAFATAEKRAATASGLKIA"
     gene            complement(5660..>5935)
                     /locus_tag="AC16CM_14445"
     CDS             complement(5660..>5935)
                     /locus_tag="AC16CM_14445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3141080.1"
                     /translation="ETFSGVDFETGLEAAEELKELVPGGHTLAQFALRWILMHPAVSC
                     AIPGGKNPAQVEDNVAAAQMPALSDEAMQRAREIYDRSIRPEVHHLW"
BASE COUNT         1047 a         1918 c         2027 g          943 t
ORIGIN      
        1 cagaagactg tagcccgccc gggcaaccgc tcggtccgcg gcaccgctgg cccccagcgc
       61 cgcgccttcc aaccacgcga gagcgccgac cggcctaccc actatctctt cgagatcttg
      121 cttcgaggct gacgcttcgc gttccagggt aaccggcctg aggcccggaa ccgccccggg
      181 agaggcgctc acatgagtcc gcgtgtggga cacgacgacg tgcccgcgct ccgcgagatc
      241 gcgcagttct tcgggcgcga gggcgagcct gccgtcaggg ggcagatcct ccccgtggta
      301 cgggaggtcg atcgcgtggc gccaggcgaa ggagcgctgg tcttctgggg gggccgagat
      361 ccagcccgag acgacgaaga accagccgac catgttcaga cggtcgagga tcggggctac
      421 gaccccgaag ttatcgcgga agccgtcgaa gaagttcagg atcacaccgg gccggtcgtg
      481 gtgccaatcg ccggtctgca cgagacgggc aaggtcctcg taagagacgg gggcgaacgc
      541 ctcggcgagc cgggagagtt gctcctccag ttgcccggct tgcctctcgg gcgtcgcgtg
      601 gaagttgacc gcccgcacgt agcgtccgcc cgccagcgcc cggtacaggg cgtggtccgg
      661 ggacgccggg cccgctgtgt ccagggcgcg ggggaagaag tcctcggcgg cgagctcggc
      721 gagcgtgact gcgaacccgc cgggccacgg cctaacctcc ggcggcggaa ggcttccctc
      781 tatgcccata gcaagcgccc cgagcctctc atcctcgtca tcccggtgcg agctccgaac
      841 ctcgtcatcc gctgctcctt cgagcagcag gaaggtgggg atccccttcg tcgccagcac
      901 ctggaacttc gtgggccctg cgacgccctc gttcatcaga gcacgccgct cgctacaagc
      961 ccggccacat ggcgagaggg ttcgcggtag agcgggtaga gccgccctac ccgcttctca
     1021 agcagatcca cgttcagcca gggcgccggg ccgaactcct cgcgggcctg cccgggcagc
     1081 aggatgggct tcgcgccggg aggcggttcg tcgacaccaa ccaggtcatc gtcccgacag
     1141 agcaggcgtc tgaggttgcg ggctccgatc cgggcgtagc tcgccgtctc gacttcgagc
     1201 cccggcacga tggccctgac cacccgaacc tctccgccgg gtggggacag gtccacgtag
     1261 agcgggtcga gcccgtcctc acggaggcgg ttggccacga ggtttgcggg ggagttcgag
     1321 tcctcggctc ccccaggaag ggttggcaat gatgagaaat ccacctccga gcgggcggcg
     1381 aatacgggat cctcgagcaa cgcgcgcatc tcgtaggcgt ccaggccaag ccagtctaca
     1441 gccccccgcg tggccctctc ttcttcggac tcgagcgtgg ccgccctgat agcgcggccc
     1501 acgtaggtag cgggcgccac ggcggtcatg cgggagagcg ggccgttgtc gaagggcttg
     1561 cgcgcccgtc cggcgcagaa ctccatgagc gccttcgaaa gcgccttctc gcggtccggg
     1621 tcagctgcct caccgcaggc gctcagggcc agggggtgcg gggctcgaac ggggtcgcgg
     1681 tccgcaccaa ccacgtagat gttgttgatg ccgaagtccg tggaggcgag cttgaccacc
     1741 acctcgatgc cgtgccggtc gagcttctcc agcagcgccc tcgtctcagg cttatcgacg
     1801 gcctcgagcc ccgagatcac gacaccctgg tcaagggcgc ggtaggcgac gctgttgccg
     1861 tcgcgttgca cgagctccag gagcccgtgg gccatggcgt gctctacggt ggggccggcc
     1921 ccgagcccgt tggtcacggg ctggacgagc aattctccct cccagtcgat gtctaccgac
     1981 gaggccgcgg cgagctccag tggaacgtag actggctccc ctgtggtcca gcgtcgggcc
     2041 agcacccagc acagcggcct ctcgggggag tacccgctcc cggcgggcag gcagacggtc
     2101 agcgggtcga ttaccccctc cctgcccacc tcggggacga gatctcggta ggaggcgcgt
     2161 cgcggggtca tggccctgac ggcatcggcg gcgaagagtg tctcggagag ctccccgaaa
     2221 gcccctgtga cggcgcgttc gtcgctctcg ccgtatccag cgccgtgatg cgccgtgccc
     2281 ctcgcggtga agagctctgc ggaccagacg ggtaggccga ggtggtccag cgggtccagg
     2341 ggataggcga aggcctcccc gtccggcagc gcgcgccggt aggccgcgac ctccggcggt
     2401 tccgaaacgg tgcgttgggg cggttcgaag agggggacgg gctcgggcaa cggctactct
     2461 ttcagggagc cgctgaagac ccccgagatg ataaagcgct ggaaaaagaa gaacagcagg
     2521 atcaggggta cgagccccac cacggcggcg accataacag gcccgtaggg tacggccccg
     2581 ccctggcgca ccaggctggc gagcatcagc gggtaggtga accgttcggg cgtgctcatg
     2641 accaccaggg gccagaggaa gttgccccac tggctcatga acgtcacgat gccgagggcc
     2701 gcacacgcag ccttcatgtt cggcaggacc acgcggaaga acacgccaag ctccccgttg
     2761 ccgtcgatgc gggcggcgtc gagcaggtcg ttcgggaagc ccatcatctg ctggcgcatc
     2821 aggaacacgc cgaaggccgt aaccaggtag ggcactatca gggcggcgaa gttatcaacc
     2881 catccgagtt gcagcatgat tatgaagagc ggcacgatca tgatctccgt cggcagcatc
     2941 agcgtcccga gcacgaccgc gaacagcagc ttgcggcccc tgaaggcgaa cttggcgaac
     3001 gcgtatccgg cgagcgccga gagcaggacg gtgctaacgg tcgtcaggcc agcgacgacg
     3061 atgctgttca gcatggcccg caagaacggc gcggcctgcg agagcgcgtc caggttggtg
     3121 aggaagtgcg agccagccag aagacgcggc ggggcacgga agatctccga ctccgtcagc
     3181 gtggaggcgg tgaccatcca gtagaacggg taaagcgtaa ccagggcccc cacgccgagg
     3241 aacacgtacg ccagcagctt caccggctcg ctcgtggtgc ggcgcgttcc cttcacctca
     3301 gacctccccc cagcgggcca gcctgagctg ggcgtaggac atgatggcca ctatggcgga
     3361 gagcacgtag gtgcccgcgg cggcgagacc gaagtcgaag cgggcgaagc ccgtgtcgta
     3421 gatatagtag aaggaagtga gcgtcgcgtt ggccgggcca ccactcgtca ggatgtaggg
     3481 ctccgtgaag agctggagcg ttccgatcgt gccgagaacc gtgcagaaga ggatcaccgg
     3541 cctcagcaag ggcaccgtga tccggaggaa gcgcaccacg ctccccgcac cgtccacgct
     3601 cgcggcgtcg tagaggtcct tcggtatgtt ctgcagcccg gagagcagga taacggcgtt
     3661 gtatcccgtc cagcgccagg tcagtgcgag gatgatcagg acccgagccc agaaggggtc
     3721 cgagcgccac ggtatcccgt ccaggcccac gaggccgagc gcctggttca cgatcccgta
     3781 ctgttcggag aagatcaggg agaagaccac cgagtaggtg acgaggtcga tggccaccgg
     3841 caggaagaaa gccagtcgga agaaggcctt gcgcctgcgc ccgagaaagt ccgagtcgat
     3901 tacggaggcg atgatggtgg ccagcccgat catgagcggc acctgtacca cgaggatgag
     3961 gcctgtgttt agcagggact tcaggaacaa cccgtctgcg agcaaccgcc tgtagttgtc
     4021 gagtccggtg aagatgatcc tgccgccgct gaattgcccg aagctgagcg cggccgagta
     4081 caggataggg taaaggatga acgcagcgaa cacgaggaag aagggcgcta tgaaagcgta
     4141 tggggcccag gtggaccagc ccgcgcgcgt tctactcttt cgggagaccg tcaaggcctc
     4201 aggctatctt caggccggag gccgtcgcgg cccgtttctc ggcggtggcg aaagcctcct
     4261 gcggggtggt atccgtggtt aggatcgagg cgtaggcgtc catgagtggt ttctggaagt
     4321 ccaggaagtg ggggccatac tccagggcct gcacgttgcg ggctatgtcc gcgaacttgg
     4381 gggctatggc gaacccgaag tagggatcgg gttcgttgaa ggccgccgtg tcccagtagg
     4441 gttcccagga ggggaagagg ccctgcgccc acgacttgct ctgaccctcc tttgtaagca
     4501 gcgcctcgtg gataaagtcc caggccgcgt ccacgtcctg cgactgggcg gagatgacga
     4561 ggacggagcc gctcagggtc gcctcctgcg ggcctcccga gtcgaaggca ggaaggggca
     4621 gcacgtccca tttacccttc tgggacttgc cgccggcgcc cttgatgagg cccacgtccc
     4681 aggcggcccc caggtcggtc gcggtgtcgt cgttcgagat ggcgcggctg agctcgtcgt
     4741 aggtcggcgt gtcgacggcg atgttctcgt tcacgaagcc gtcgagtacc tccatcgcac
     4801 tgtagctctt ctcgttgctg aaatctatct tgccgccgtc ggagtagaac ccgccagact
     4861 gctggttgag cagtagctgg tagtaagagg aattggtccc cccaccggag gcgtcgaacg
     4921 ccgtcatctt ggccccggtc ttccgctcca gctcctggcc ggcggcgacg aagtcgtcgt
     4981 aggtagcaac gtcgtccggc ccgatgccgg cctgctcgaa gagatccttt cggtaccaca
     5041 tcccaaccgg ccccatgtcc cagggcaccc cgtagagctt gccgtccttg cgggcgtgct
     5101 ccagcggggc ctcagcgaac tgtccctcat actccgccag cctgtccgta acgtttacga
     5161 actggccggg gaagcgctcc agaaagttct ggaagtcctg ctggccgagc gaaaagatgt
     5221 cgggggcgcc gctgccggcc tgcaagcggg ggatgatctg ctggtaatcg atggttatgg
     5281 actgcatccg tacgggcgtg tccggtcgct ttttcttgaa gagcgggacg gtgttcttga
     5341 gggcatccct ggccacatcc caggaggcga ccgtcaggcc agggctgtca gaggagccgc
     5401 agcccaccaa cgggccgccg ccaagcaacg cggcgccggc aagccccacg ccacccagct
     5461 tcagaaacct ccgtcggctc atgccgccgg tcatgtctag gcccgctccg gtatgcctgg
     5521 tgtcacccat tctcgatccc cctcggacgc tttcagacca gggccggcgt gcgaccctcg
     5581 acctttctac atgggtggac ggcagattta tgtattcgcg tctacgaaag tacgcgcgcc
     5641 agccaaagtg gccggcccac taccagaggt ggtgaacctc gggccggata gaacggtcgt
     5701 agatctcgcg cgcccgttgc atggcctcgt cagagagtgc cggcatctgc gcggcggcca
     5761 cgttgtcctc tacctgggct gggttcttgc cgccggggat ggcgcaggag acggcggggt
     5821 gcatgaggat ccagcgcagc gcgaactgcg cgagggtgtg ccccccgggc acgagctctt
     5881 tcaactcctc cgcggcctcc aggcccgtct cgaagtcgac cccggagaag gtctc
//
LOCUS       DBNGVD010000535         7157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1232657, whole genome shotgun sequence.
ACCESSION   DBNGVD010000535 DBNGVD010000000
VERSION     DBNGVD010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1232657"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..354
                     /locus_tag="AC16CM_14450"
     CDS             43..354
                     /locus_tag="AC16CM_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3141081.1"
                     /translation="MHSVAIPSASSTETRPGIEIEQSTTEEPPYKVILHNDDYTPMEH
                     VVAVLQKVIPRMSVQRAVDIMMEAHNRGKAVATKCHKELAELYQEGLNNEGLIASIEP
                     D"
     gene            complement(361..1347)
                     /gene="moaA"
                     /locus_tag="AC16CM_14455"
     CDS             complement(361..1347)
                     /gene="moaA"
                     /locus_tag="AC16CM_14455"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3141082.1"
                     /translation="MTQLTDSFDRKMDYLRISVTDRCNFRCQYCMPEDIQFQDKSQIL
                     TLEEMLTFAEACLQLGVTKVRVTGGEPLVRRGVVMFIGWLKDLGFEDVTMTTNGFLLE
                     ENLEGLVEAGLDRVNISLDTLQPEKFAFITRRNHFEKVWKALMAAIETTLSPVKINAV
                     AMRGVNDDEISDMARLTLEYPMHVRFIELMPLNGDTDGSRFRSLFIPGIEILERAQAE
                     LGDLVPVEKDDPAAPADEFRFDGAPGTFGVITPVSEPFCARCSRLRLTADGKIHPCLL
                     TDLDVPVKDAIRSEDPIPAIHKVLWETARIKPAAGLTLPEEARHRTMSQIGG"
     gene            1489..2808
                     /locus_tag="AC16CM_14460"
     CDS             1489..2808
                     /locus_tag="AC16CM_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3141083.1"
                     /translation="MFRLTAPRLIVYGFLALVGAGTAMLMLPWSTTGGISLTNAFFVA
                     TSCVTVTGLSTVGIPGTFTHFGQVMMMILMQLGGLGIMTVTTLAALLIGQRVGFRRLL
                     TVRTETESAGSPRNTLRLLFQIARITFAVELLGAVVLSIGFVSVGLGLGEGVFQGIFH
                     AIMAFCNAGFATIPDGDLVPYAGNWLIIGGLAAVITLGGLGFPVLVDLYNFREDRRLS
                     VHSRVVLITSAILIVVGVASVALFEWTNPNTLGGQSLNSKAAMSLLQGVTPRTAGFTT
                     VSYPEMRDDTLLIQMILMFIGTAPTSTGGGIKVTTLALVALIVVAQVRGQDRITLFSR
                     TLPRPLVARALSILALASLLVISSTLALMVSDDLNLLPALFEVTSAFGTVGLSLDVTP
                     QLSPFGKLLISLLMFLGRVGPITFILALAARERTPRYRYPEEEIAIG"
     gene            3020..3490
                     /locus_tag="AC16CM_14465"
     CDS             3020..3490
                     /locus_tag="AC16CM_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3141084.1"
                     /translation="MGVEERSEPDIRIEDIMRPADTVRPDTLARDALKVMREHGVPGV
                     AVTDEDGTLRGFITDGQLLEAALPRYLKMMNRLSFVREDADRWVHYLSESADRPVGEV
                     MSKDVSSIELDRSELAVAHRMVHDGVSSVVITRDGKVEGIVNRQDLYAAIVGIE"
     gene            3606..4877
                     /locus_tag="AC16CM_14470"
     CDS             3606..4877
                     /locus_tag="AC16CM_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3141085.1"
                     /translation="MIFALAVFLLVLAVFAIDLVHRTPAALAGAVLLVMAGAIGQDEA
                     IEAVHWDTLGLLVGMMILVGILKDSGIFGYLAIKSAQAARGRPGIVMIYLAAITALLS
                     AFLDNVTTVILMFPVTLVISRILEEDPVPFLIVEVLASNIGGTATLVGDPPNIIIGTV
                     VDGLTFMDFVVNLAPPILVILLVTFGIVWVVYARKLSTTEEDREGIMALEAGEEIEDR
                     TLLVRAGAVSGATVVGFFLQEITGFNPAIVALAGAAVAMLVCGPEVEKVLHEVEWPTI
                     LFFVGLFVMVGALETTGFIDTVAHALASASESEAGTAIMVLWGSGFASGIVDNIPFTA
                     TMIPVIQQIAEARGYAPSEVEPLWWSLSLGACLGGNFTLIGASANLVVAGLVAREGLT
                     SFNFLRFLLWGFPLTLVALVISTFYILVFQL"
     gene            complement(4879..6432)
                     /locus_tag="AC16CM_14475"
     CDS             complement(4879..6432)
                     /locus_tag="AC16CM_14475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3141086.1"
                     /translation="MKLIMTHGYMLSGTGSNVYVQSLCRALVGEGHELHVICQEPDPL
                     SFDFVNAHATVDADGVHEQGERETSFPGRCTVYVPDIGDLLPVYVYDEYEGWRVKTFL
                     DLTQAELDNYLDRNVGAVQSVLGASEAEGVIVNHSVPGPLIARRALQGSGVNYVSIVH
                     GSCLQYLSRRSEKYMGLTREGLEGAAEILALSSHSVGTVVEDFPDLADRIEALPGGVD
                     TGLFSPDAFDLGGVEKLNGGPGRGPDQRSAVEEALGRSGNATELAEALRDVAASYDAR
                     AHDSDAGERIRRFLDREGPVVVYVGKLIHSKGVHSLLSALARVRKQTGARLLVVGFGT
                     FREGLEALTLALSEGDESTVERLAEMGKLLEGGTAGPLEHFELSDELLADAAGMAEDV
                     LFTGAIYHGDLPKLLPAADVASVPSIFPEAFGLVAAEFAASGVVPFVANHSGLEEAGG
                     LVGKDLPFDLRVGMDGFEENLAGALTDYLQLPDEERQRCEEIVRRNSVEDLSWGTLAE
                     KLVGLVAGR"
     gene            6593..>7157
                     /locus_tag="AC16CM_14480"
     CDS             6593..>7157
                     /locus_tag="AC16CM_14480"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3141087.1"
                     /translation="MFSRVKVGPVPGVGDEAPEFTLPSAQGGQFRLSMRTVRGPVVVA
                     FYRPGDEEDVEYFKALAGKEEEIHLASASVVAIGVAEPDQARNFARASGLKSYVLYDY
                     ARVASTQWGLLERDKKEGDYARPAVFLVGPDHEVTQAWVGQRPDPEEILARVSEITGL
                     PKPPEEPKEEGQEKADEGEKPKKMSAEE"
BASE COUNT         1272 a         2235 c         2286 g         1364 t
ORIGIN      
        1 cagtcagtca gaccggagat gacagtgcaa agcataccaa cgttgcatag cgtagcgatc
       61 ccctctgcct cttccaccga gaccagaccg gggatagaga tagagcagag taccaccgag
      121 gaaccaccct acaaggtcat cctacacaac gacgactaca cccccatgga gcacgttgtc
      181 gccgtactac agaaggtcat cccgcgcatg tcggtacagc gggccgtgga catcatgatg
      241 gaggcccaca acaggggcaa ggccgtcgcc accaagtgcc acaaggagct cgccgaactc
      301 taccaggagg gcctcaacaa cgagggcctg atcgcttcaa tcgagcccga ctaggagggc
      361 ttaaccgccg atctggctca ttgtgcggtg gcgcgcttcc tctggaagcg tcaaaccagc
      421 ggcgggcttg atgcgcgccg tctcccagag caccttgtgg atcgccggga tggggtcttc
      481 cgagcggata gcgtccttta ccggcacgtc gaggtctgtc aggaggcaag ggtggatctt
      541 gccgtccgcc gtgagccgca ggcggctgca gcgggcgcag aagggctctg agactggggt
      601 tatcacgccg aaggtgccgg gggctccgtc gaatcggaac tcgtcggccg gggcggcggg
      661 gtcgtctttt tcgacgggca cgaggtctcc cagctctgcc tgggcacgtt ccaggatctc
      721 gatccccggt atgaagagcg agcggaagcg tgagccatcc gtgtcgccgt tcaggggcat
      781 gagctcgatg aacctgacgt gcatggggta ttcgagcgtc agcctcgcca tatctgagat
      841 ctcgtcgtcg ttgaccccgc gcatggcgac ggcgttgatc ttgacgggcg agagcgtcgt
      901 ctcgatggcc gccatcaagg ccttccagac cttctcgaag tggttgcggc gggttatgaa
      961 ggcgaacttc tcgggctgaa gcgtatcgag cgagatgttt acgcggtcga ggccggcctc
     1021 cacgagaccc tccaggttct cctccaggag gaatccgttg gtggtcatgg tcacgtcctc
     1081 gaagccgagg tccttgagcc acccgatgaa cattacgacg ccgcgcctga cgagcggctc
     1141 gccacccgta acccgtacct tggtcacgcc gagttgcagg cacgcctcgg cgaaggtaag
     1201 catctcttct agggtaagga tctggctctt gtcctggaac tggatgtctt caggcatgca
     1261 atactggcaa cggaagttgc agcggtcggt gaccgagatc ctgaggtagt ccatcttgcg
     1321 gtcgaagctg tcggtaagct gcgtcaaaat cgcccttcga gtctacagcc gatcactcgc
     1381 ttgcctgatt gtatctcttt tcgtggatgg tagggtcgcc tgctagcgac cggcccgcgg
     1441 gtatactgct tcgagctgtc aggcatcacg gagacacggg agagggtttt gtttcgtctt
     1501 acggccccgc ggctcatagt ctatggcttc ctggcgctcg ttggtgccgg gacggcgatg
     1561 ctcatgttgc cctggtccac taccggcggc atctccctga cgaacgcgtt cttcgtcgct
     1621 acgagctgcg taactgtaac cggtctatcg acggtcggca tcccgggtac cttcacccac
     1681 ttcgggcagg tgatgatgat gattctgatg cagctcggtg gtcttggcat tatgacggtt
     1741 accaccctgg ccgccctgct catcggccag agggtgggct tcaggcgtct cctcaccgtg
     1801 cgcacggaga cggagagcgc cggctccccc cgcaacaccc tcaggctgct cttccagata
     1861 gccaggatca ccttcgccgt ggagttgctc ggggccgtgg tcctgagcat agggtttgtt
     1921 agcgtggggc tgggtctcgg cgagggtgtc ttccagggca tcttccacgc gatcatggcc
     1981 ttctgcaacg ccgggttcgc cacgataccc gacggcgatc tggtccccta cgcgggtaac
     2041 tggctcataa tcggggggct tgcggccgtg atcacgctcg ggggtctcgg ctttcccgtg
     2101 ctggtagacc tctacaactt tcgtgaggat cgcaggctct ccgtacactc cagggtggtc
     2161 ctgatcacct ccgcgatcct gatcgtggtc ggcgtcgcga gcgtggcgct cttcgagtgg
     2221 acgaacccga acacgctggg ggggcagtct ctcaactcga aggccgccat gtccttgctc
     2281 cagggggtaa cccccagaac ggccgggttc acgaccgtca gctaccctga gatgcgcgac
     2341 gacacacttc tcatacagat gatcctgatg ttcataggca cagccccgac ctcgacgggg
     2401 ggcggcatca aggtgacgac cctggcgctc gtggccctta tcgtggtggc ccaggtcagg
     2461 ggacaggaca ggatcaccct cttctcgcgc actcttccgc gaccgctggt cgcgagggcc
     2521 ctctccatcc tggcccttgc ctcactgctc gtcatctcga gcacgcttgc ccttatggtc
     2581 tcagacgacc tcaacctcct gcccgccctc ttcgaggtaa cctccgcctt cggtaccgtt
     2641 ggcctcagcc tcgacgtaac gccccagttg agccccttcg gcaagttgct gatctccctc
     2701 ctgatgttcc tgggaagggt cggacccata accttcatcc tggccctggc cgcccgcgaa
     2761 cggacccccc gctacaggta tcccgaggaa gagattgcga taggttgagg tgggcccgtc
     2821 gtagtacccg agcacgatgt aaaccgtggc catagccagg gagacgaagg taacaggaac
     2881 cccggagaga tggcaatccc gagcagcgcg gcgatggtcc tgtctactac cacgaacgcg
     2941 atgagagcga tggccaagac ttcatgcacg atggttccgg attatacaat tcaggaagga
     3001 gccaaaagag gaggccgcaa tgggcgttga ggagaggtcg gagccggata tcaggatcga
     3061 ggacatcatg cgtcctgctg acaccgtgcg ccctgataca ctagcccggg acgccttgaa
     3121 ggtgatgcgc gagcacggcg ttcccggcgt cgcggtgacg gacgaggacg gcaccctacg
     3181 tggcttcatc acggacggcc agcttcttga ggcggcgctt cccaggtacc tgaagatgat
     3241 gaacaggctc tccttcgtcc gcgaggacgc agacaggtgg gttcattatc tttccgagtc
     3301 cgcggatcgg ccagtaggag aggtgatgag caaggacgtg tcgagcatag agctcgacag
     3361 gagcgagctt gccgtggcgc acagaatggt acacgatggc gtctcgagcg tggtcatcac
     3421 aagagacggc aaggtcgagg ggatcgtgaa tcgccaggat ctgtacgccg ccatcgtggg
     3481 aatcgagtag tggagagagg tttttaggag cgggcgcctt ccgcctgcta ggatgcccat
     3541 caggcgtact ttagcggcgg ggggatgggg agcttgggca acgggtctga cggggtagcg
     3601 acgtggtgat cttcgcgctc gcggttttcc tcctcgtgct ggccgtgttc gccatagacc
     3661 tcgtccaccg cacgccggcg gccctggctg gcgcggtact gctggtgatg gcgggcgcga
     3721 tcggtcagga cgaagccata gaggcagtcc actgggacac gctcgggctc ctggtgggga
     3781 tgatgatcct ggttggcatc ctcaaggact cgggcatctt cgggtacctg gccatcaaga
     3841 gcgcccaggc ggccagaggg cggcccggta tcgtcatgat ctacctggcc gcgatcacgg
     3901 ctctcctctc cgcgttcctc gacaacgtga caaccgtgat cctgatgttc ccggtaacgc
     3961 tggtcatctc ccgcatactg gaagaggatc ccgtaccgtt cctcatcgtc gaggtgctcg
     4021 cgtcgaacat aggggggact gcgacgctcg ttggagaccc gcccaacatc atcatcggga
     4081 ccgtcgtcga tgggcttacc ttcatggact tcgtggtcaa cctcgcaccg ccgatcctgg
     4141 ttatcctgct cgtgacgttc gggatcgtct gggtggtcta cgcgaggaaa ctgagcacga
     4201 ccgaagagga tcgcgaaggg atcatggccc tggaggcggg tgaagagata gaggaccgca
     4261 cgttgctcgt gcgcgcggga gcggtgtcgg gcgcgaccgt ggtcggcttc tttctacagg
     4321 agattaccgg cttcaacccc gccatcgtgg cgctcgccgg agccgccgtc gccatgctcg
     4381 tctgcggccc cgaggtggag aaggtgctgc acgaggtgga atggccaacc atactcttct
     4441 tcgtcggcct cttcgttatg gttggcgccc tggagacgac ggggtttata gacacggtgg
     4501 cgcacgctct ggcttcggcc tctgaatccg aggcgggcac ggccattatg gtgctgtggg
     4561 ggagcggttt tgcctcggga atagtggaca acatcccctt cacggcgacc atgatcccgg
     4621 tcatacaaca gatcgccgag gccagaggct acgcaccctc ggaggtcgag ccgctgtggt
     4681 ggtcactctc ccttggcgcc tgcctcggcg gcaacttcac cttgatcggg gcctctgcca
     4741 acctggtggt cgccggcctg gtggcacggg aaggactgac ctcgttcaac ttcttgcgct
     4801 tcctgctctg gggtttcccc ctgacgctgg tcgccctggt gatctcgacc ttctacatcc
     4861 ttgtcttcca gctctaggct atctcccggc cacgagccct acgagcttct cggccagggt
     4921 tccccagctc aggtcctcga cgctgttccg gcgcacgatc tcctcgcagc gctgccgctc
     4981 ttcatccggt agctgtaggt agtcggtcag cgccccggcc aggttctcct cgaagccgtc
     5041 catcccgacg cgcaggtcga agggcagatc cttcccgact agcccgccag cctcttccag
     5101 gccggaatgg ttcgcgacga agggaacgac cccgctcgcc gcaaactcgg cggccacgag
     5161 gccgaacgcc tctggaaaga tggaaggcac gctcgccacg tcggccgccg gcaagagctt
     5221 cgggagatcg ccgtggtaga tggcgcccgt gaacaggacg tcttccgcca tgccggcggc
     5281 gtcggccagc aactcgtcgg agagctcgaa gtgctccagg gggcctgcag tcccgccttc
     5341 gaggagcttg cccatctcgg ccaagcgctc caccgtggac tcatcgccct cgctcaaagc
     5401 aagcgtcaac gcctccaggc cctccctgaa ggtgccgaac ccgaccacca gcagccgcgc
     5461 ccccgtctgc ttgcgtaccc tggcaagggc cgagagcaag ctgtggactc ccttggagtg
     5521 gatgagcttc ccgacgtaga ccaccacggg tccctcgcgg tcgagaaacc tcctgatccg
     5581 ctctccggcg tcgctgtcgt gcgcgcgggc gtcataggag gcggccacgt cacgcaacgc
     5641 ttcggccaac tccgtcgcat ttcccgagcg cccgagggcc tcctctacag cggaccgctg
     5701 gtcggggcca cgcccggggc cgccgttcag cttctctacg cccccgaggt cgaaggcatc
     5761 ggggctgaac agacccgtat cgacgccgcc ggggagcgct tcgatcctgt cggcgaggtc
     5821 ggggaagtcc tcgaccaccg tgccgacgct gtgtgaggag agcgccagta tctccgccgc
     5881 gccctccagc ccctccctgg tcaggcccat gtacttctcg ctccggcggg agaggtactg
     5941 caggcagctt ccgtggacga tgctcacgta attcacgccg ctgccctgca gggcccgcct
     6001 tgctatcaag ggacctggta cggagtggtt gacgatgacc ccctccgcct cagatgcccc
     6061 gagtacgctc tgtacggccc cgacgttgcg atccaggtaa ttgtccagct ccgcctgggt
     6121 caggtccagg aaggtcttga cgcgccaccc ctcgtattcg tcgtatacgt agacgggaag
     6181 gaggtcccct atgtccggca cgtagacggt gcagcggccg ggaaaggagg tctctctctc
     6241 accctgctcg tggactccat cggcgtccac cgtggcgtgc gcgttcacga aatcgaagga
     6301 gagcgggtcg ggctcctggc agatcacgtg cagctcgtga ccctcaccga ccagggcccg
     6361 gcaaaggctc tggacgtaga cgttgcttcc cgtccccgag agcatgtacc cgtgcgtcat
     6421 gatcaacttc atagctcgtc ctcctccgtc ccctgtagag tctccatgcc cgcaaagcgg
     6481 aaggtaacac agcatgaacc taccggccca ggcggcacgg tccgcggccc agagccgaag
     6541 cgtgctgtag tattcgtgcg tacgcacgaa tactactcgc aaggagggtt gaatgttttc
     6601 acgggtcaag gttgggccgg tgcctggagt gggggacgag gcccccgagt tcactttgcc
     6661 aagtgcccag ggggggcagt tcaggttgag catgaggacg gtcaggggac ctgtggtcgt
     6721 ggcgttctac agacccgggg acgaggagga cgtcgaatat ttcaaggcgc ttgcggggaa
     6781 ggaagaggag atccatctgg cctcggcctc cgtggtcgcc atcggggttg ccgagcccga
     6841 ccaggcgcgt aatttcgccc gtgcttcggg gttgaagtcc tacgttctct acgactacgc
     6901 aagggtcgcg agcacccagt gggggttgct ggagagggac aagaaggagg gcgattacgc
     6961 tcgcccggcg gtgttcctgg ttggccccga ccacgaagtg acccaggcct gggtcggcca
     7021 gaggcccgac ccggaagaga tactggcgag ggtaagcgag attaccggcc tccccaaacc
     7081 acccgaggaa ccgaaggaag aagggcagga gaaggccgac gagggcgaga agccaaagaa
     7141 gatgtcggcc gaggagc
//
LOCUS       DBNGVD010000536         3495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1597200, whole genome shotgun sequence.
ACCESSION   DBNGVD010000536 DBNGVD010000000
VERSION     DBNGVD010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1597200"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="AC16CM_14485"
     CDS             complement(<1..1537)
                     /locus_tag="AC16CM_14485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3141088.1"
                     /translation="MAPRGAGLLRMGLAAVVAVLLSQGWSPAGMAGAYLAVSLAFLLG
                     LFEAVGWPGILVRERLVCVFLDCALVGVLIAYTGGAESPFFLLLFLAALGVAWTDTRQ
                     KVAAGTAALVAGYPVAVVAAGGFGDLVSTPVALKTGFVALFCVAVTFLATHVQGVKKL
                     AVKLSSTLANEIDRVERDESLISEFSPVLKVLSLEGILRWTVGAAHVLGGGPYAHVAS
                     LTGNHHHTLMEGDSEACPSWWHPTIQSLVLQSCRGGEVVRSEEEIHGISGFLVVPLGK
                     DEEEKWGAVIVGGGEFGAEEERALKLLAGEVAPVLENTAEAPGGLDHLTGLPNRASLR
                     RVLRQELSYGRPLTVLAMHPAGLRGHASSTLIREIGEKLANGRQRAFRYGDEEFVVVL
                     GGSGEDRARRVARSLRQHLSEGTGAVGNQPWVTVGWAIAETGDEYAAIGAALQALQKA
                     DERPEGISGLPASAQDPGELGGERQVSGIVRTLVEAMEAKDPSIKEHLKSVSSLSLRI
                     GREM"
     gene            complement(1602..2918)
                     /locus_tag="AC16CM_14490"
     CDS             complement(1602..2918)
                     /locus_tag="AC16CM_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3141089.1"
                     /translation="MEPQLIVGAAIFAAFIFLGDLFAIRVNERTSIGVWNIPVIVAVA
                     SLGPEWAALASIPSAVFVGWRDPLRTVHEISHNVTIIYLAGMAFSLISAPLLVDDSAP
                     WASILYATLVAGIVQEVSSELINGFLLRVKYNQSFHKTWQNTVQPYLLPEAVNILTAG
                     LAILALVVYDPVAAVVVVAGSIGSLALMYRSREQVKENEELRARVKSLEAALANSNTA
                     FGTMIIRDLGQRDGYTHRHAAATATYAADLAREMKLDDTHVEKLRMAGLLHNIGLFGL
                     PDEMLLATGRPNSIARRHLDEHPARGEEALAGIAEFEEMASWVRWHHERPDGRGYPDK
                     LRGPWIPLEARILSVAQAYAAMILDQPRRAGMTSGQAREQLSAGLDTEFDGVVVRALL
                     RLLDTESEGYRMADDHRFVFPSPEGGVRSGTPEPRRDEHLGRIVPH"
     gene            complement(3301..>3495)
                     /locus_tag="AC16CM_14495"
     CDS             complement(3301..>3495)
                     /locus_tag="AC16CM_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141090.1"
                     /translation="RVFSVRFLGFSVGEILAYGVGGALVDASGARPTYLLAAAATAAA
                     GLVILAVLALASDTRAEETQ"
BASE COUNT          628 a         1195 c         1057 g          615 t
ORIGIN      
        1 acatctcgcg gccgatgcgt aaggagaggc tggagacgga cttcagatgc tccttgatgc
       61 tggggtcctt ggcttccatg gcctcgacga gcgttctgac gatccccgat acctgccgtt
      121 ctcccccgag ctccccgggg tcttgcgcgc tggcgggtag tcctgagata ccttcgggtc
      181 gttcgtccgc cttctgcagg gcttgcaggg ccgcgccgat cgcagcgtac tcgtccccgg
      241 tctcggcgat cgcccagccc acggtgaccc agggctggtt gccgacggcc ccagtccctt
      301 ccgagaggtg ctgccggagc gagcgggcga cccgtcgcgc cctgtcctcg cccgagccgc
      361 caaggacgac aacgaactct tcgtccccgt agcggaacgc gcgctgcctg ccgttagcga
      421 gcttctctcc tatctcgcgt atgagggtgc tgctggcatg gccgcgaagc cctgccggat
      481 gcatcgccag caccgtaagc ggcctgccgt aggagagctc ctgccgaagg acacgccgca
      541 gcgaggcccg gttgggcaaa cccgtcagat ggtccagacc gcctggggct tccgcggtgt
      601 tctccaggac cggtgccacc tccccggcca ggagcttcag ggctcgctcc tcctcggctc
      661 cgaactcccc gccgccgacg atcacggccc cccacttctc ctcctcgtcc ttgccaagtg
      721 gcacgaccag gaagccgctt atgccgtgga tctcctcctc gctacgcacc acctcccccc
      781 cacgacaact ctggagcacg aggctctgga tcgtggggtg ccaccagctc gggcaggcct
      841 cggagtcgcc ttccatgagg gtatggtggt ggttgccggt gagcgaggcg acgtgcgcat
      901 aaggtccccc gcccagcacg tgggcggccc ccacggtcca ccggaggata ccctccaggc
      961 tcaacacctt cagcacgggc gagaactcag agatcaggct ctcatctctc tccacgcggt
     1021 ctatctcgtt ggccagggtg gaagagagct tcacggctag cttcttgacg ccctgtacgt
     1081 gggtggccag aaacgtaacg gccacgcaga agagcgccac gaacccggtc ttcaaagcca
     1141 ccggggtaga gacgaggtcc ccgaacccgc cagccgcgac gacggcgacg ggatatcctg
     1201 ccacgagcgc ggccgtgccc gccgcgacct tctggcgggt gtcggtccag gcgaccccca
     1261 gtgccgccag gaaaaggagc aggaagaagg gggactcggc ccctcccgtg taggcgatca
     1321 gtacccctac gagcgcgcag tccagaaaca cgcacaccag ccgctcgcgc accagaatcc
     1381 cgggccatcc cacggcctcg aacaacccga gtagaaaagc caggctcacg gccagataag
     1441 cgcccgccat ccctgccggg gaccacccct ggctcaacag cacggccacg acggccgcca
     1501 gccccatacg cagcagaccc gcgccccttg gggccattgc ggatgccgtt ttgatctggc
     1561 gcaatctctt acctcgactg acttctacct gacgaccgat gctagtgggg aacgattcga
     1621 ccgagatgct cgtcgcggcg gggttcgggc gtgcccgacc tcacgccacc ttctggggag
     1681 gggaacacga agcggtgatc gtcggccatc ctgtaccctt ccgactcggt gtccaggagc
     1741 ctcaagagcg ccctgacgac gaccccgtcg aactccgtgt caaggccggc gctcaactgc
     1801 tcgcgcgcct gcccactcgt catgccggct cgcctgggct ggtccaggat catggcggcg
     1861 taggcctggg ccaccgacag tatcctggcc tctagaggta tccagggacc acgcagcttg
     1921 tccgggtagc ctctgccgtc gggccgctcg tggtgccagc ggacccaaga ggccatctcc
     1981 tcgaactccg cgatcccggc cagcgcctcc tcgccacgcg cgggatgctc gtcgaggtgc
     2041 ctcctggcga tggagttggg cctgccggtg gccagcagca tctcgtcggg caacccgaac
     2101 aacccgatgt tgtgcaggag cccggccatg cgcagctttt cgacgtgggt gtcatcgagc
     2161 ttcatctcgc gggccaggtc ggccgcgtac gtcgccgtgg ccgcggcatg cctgtgggtg
     2221 tacccatccc tctgccccag gtcccggatg atcatggtcc cgaacgccgt gttggagtta
     2281 gccagggccg cttccaggga cttcacgcgc gcccgcaact cttcgttctc tttcacctgc
     2341 tcccgcgagc ggtacattaa cgcgaggctt ccgatggaac cggcgaccac gaccaccgcc
     2401 gccaccgggt catagacgac gagggccaga atcgctagcc cggccgtgag gatgttgacg
     2461 gcctccggta gcagataagg ttgcaccgta ttctgccagg tcttgtggaa cgactggttg
     2521 tacttgactc tgagcaggaa cccgttgata agctcgctcg atacctcctg gactataccg
     2581 gccaccaggg tcgcgtacag gatagaggcc cagggtgccg agtcatcgac gagcaagggg
     2641 gcagagatca acgagaacgc cattcccgcc aggtaaatga tcgtgacgtt gtggctgatt
     2701 tcgtggaccg tccggagcgg atctcgccat ccgacgaaca ccgcagaggg gatagacgct
     2761 aatgcggccc attcgggacc cagcgaggca acggcaacta taaccggaat gttccacacc
     2821 ccgatagaag tccgctcgtt gacccgtatg gcgaagagat cgcccaggaa gatgaacgca
     2881 gcgaatattg ccgcgccgac gatgagttgt ggctccaacg caagaccata cttgtagccc
     2941 caggcagcaa ctaacgccgc agcgaacgct gcaagggctc ctacatatga tttgagatgg
     3001 acttcgacca aaccgtggca ccctgtaccg gaatccgcat ttgttgaacg gtatattctc
     3061 gcacacctgg caggttctac acaaggctcg gttcgtcgct cgggttttac caggttctct
     3121 tgctcattat cctcctttca cgacgcggtt gctgatggct caatctccgc tccgctatta
     3181 ccaggatcgc ttgcccatta cctgcccctt tcttgcactt tatgctacct ggatactacc
     3241 tgaacttgcg ccccgtggta agtaaccgaa ccggtaaggt gctagtactc gcgatccgtg
     3301 ttactgcgtt tcttcagccc tcgtatccga ggccaaggca aggacggcca ggatgacgag
     3361 gcctgcggcc gcggttgcgg cggcggccag gaggtacgtg gggcgcgctc ccgaggcgtc
     3421 cacgagggcg ccaccgacgc cgtaggcgag gatctcccct acgctgaacc cgaggaacct
     3481 gacggagaaa acgcg
//
LOCUS       DBNGVD010000537         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1051689, whole genome shotgun sequence.
ACCESSION   DBNGVD010000537 DBNGVD010000000
VERSION     DBNGVD010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1051689"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..809)
                     /locus_tag="AC16CM_14500"
     CDS             complement(114..809)
                     /locus_tag="AC16CM_14500"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3141091.1"
                     /translation="MNNFSRLPVTFGLIGACVTVYLAVAIAGQSYGHPLNVSLVTQPF
                     DVVGRGALVPDLVARGEVWRLVSSMFLHAGFVHLALNMLSLYFLGAFVEVTFGRARFL
                     ILYLSSGLAGGIAYLYFGAFSGSVVGASGAIFGLLGGVLGYSLRRGTFSWQNPLIRQL
                     LILTVLNLYIGFSVPNISNAAHIGGLLSGLAFGWLVAPTVYSEKKVRAATTTIIVLGT
                     EVVLLAVWFIVLA"
     gene            complement(837..1283)
                     /locus_tag="AC16CM_14505"
     CDS             complement(837..1283)
                     /locus_tag="AC16CM_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141092.1"
                     /translation="MAVEGKVAKILGNNEIVINRGSGQGVRQGMLFEVFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKERLAVARIQHTQETPFGAVDIGEMQENLQRMFGQM
                     FGDDVQVRGFGANQGDEPDLESMLGGPLQDLSKVQVGDAVREIRRR"
     gene            1370..2233
                     /locus_tag="AC16CM_14510"
     CDS             1370..2233
                     /locus_tag="AC16CM_14510"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR3141093.1"
                     /translation="MQQRTNSIGRHLPTSGGLARTLKSARELGCETVQIFVSNPQGWA
                     VPAPRGDADVLVAGAGEIGVSPVVAHAKYLINLASKDEDLRARSVRALAHEMATAGAL
                     GVDLVVVHAGSHGGDGEEVGMDRLVEGLVSAREQAAELDGASGVAEVIVENSVGAGTQ
                     LCSTFGALGEVARRAGVRVCVDTAHAFVAGYDLTSGEGGEKLASDLVAAVGDAVALLH
                     LNDAKNELGSHRDGHRKVGEGCISIEAWPGLFGGLPGVPVVMETPYETPHADLEEILL
                     VKKLAGGLRKA"
BASE COUNT          431 a          711 c          773 g          381 t
ORIGIN      
        1 cgatcccccg gccggcggaa catttatggc cgccgtcaca ccgggcaacg cttacgcccg
       61 agccatgttg gggtcgttcg agaaccgccc acaaacgtct cgatggctgt cgcctacgct
      121 aacacgatga accacaccgc caacaaaacg acctccgttc caagtacgat gatggtggtg
      181 gtggcggcgc ggaccttctt ctcggagtag acggttggcg cgacgagcca cccgaaggcg
      241 aggccgctga gcaagccgcc gatatgggcg gcgttgctga tgttagggac ggagaacccg
      301 atgtagaggt tcaggacggt caggatgagg agttgacgga tcagggggtt ctgccaggag
      361 aaggtgccgc ggcgcaacga gtagccgagc acgcctccca ggaggccgaa gatcgcgcca
      421 gaagccccca ccacggaacc gctgaaggcc ccgaagtaca ggtaggcgat gccaccggcg
      481 agcccgctag agaggtagag tatgagaaag cgcgcgcgtc cgaacgtcac ctctacaaaa
      541 gccccaagaa agtagagcga cagcatgttg agcgcgaggt gaacgaaccc ggcgtgcagg
      601 aacatgctcg ataccagacg ccacacttcg ccccgcgcca ccagatctgg cacgagcgcg
      661 cccctgccaa cgacatcgaa cggctgcgtc accaggctca cgtttagcgg gtgtccgtaa
      721 ctctggccgg cgatagcgac cgccaggtag acggttacgc aggccccgat caggccaaac
      781 gttaccggta ggcgggagaa gttattcaaa gagccgtggg ttgcgtggcg ccggggctag
      841 cggcgccgaa tctcacgcac ggcatcgccg acctgcacct tggacagatc ctggagtggt
      901 ccaccgagca ttgattccag gtcgggctcg tcgccctggt tcgcaccgaa gccgcggacc
      961 tgcacgtcgt cgccgaacat ctgtccgaac atgcgctgca ggttctcctg catctccccg
     1021 atgtccacag caccgaaagg cgtctcctgc gtgtgctgta tgcgtgcgac ggcgagccgc
     1081 tccttgacct ccgtcacctc ggccttcgcc ttcacgtcct cgaccgtgcc aagcgtctcg
     1141 ccggtgtctg gatcccagac ctcttcgcct tccggggcga agacctcgaa gagcatgccc
     1201 tggcggacgc cctggccgct ccctcggttt atgacgatct cgttgttgcc caggatcttc
     1261 gccaccttgc cttcgacagc catgtcgccc ttccttccag tagatcaaac gttcccgggg
     1321 attatatctc tcacagcggc ccgggccgcc ggcctgtaga atcgccgcca tgcagcagcg
     1381 gacgaacagc atcgggaggc acctgccgac ctcgggcgga ctggccagga ccctgaagtc
     1441 cgcccgcgag ttggggtgcg agacggtcca gattttcgtc tccaacccgc aaggctgggc
     1501 cgttccagcc ccacgggggg acgccgacgt acttgtcgcg ggggcgggcg agatcggggt
     1561 ctctccggtt gtggcgcacg ccaagtacct cataaacctg gcctccaagg atgaggatct
     1621 tcgggcccgc tcggtgaggg ctctcgccca cgagatggcc acggcgggtg ccctgggcgt
     1681 cgacctggtg gtcgtgcacg ctggcagtca cggcggtgac ggcgaagagg ttggcatgga
     1741 ccggctggtg gagggactgg tgagcgccag ggagcaggcg gcagagctcg acggcgcctc
     1801 tggggtggct gaggtaatag tggagaactc cgtgggggct ggcacccagc tctgctccac
     1861 ctttggtgcg ctcggggagg tcgcaaggcg tgccggtgtt cgggtgtgcg tggatacggc
     1921 ccacgcgttt gtcgccggct acgacctgac gtcgggggag gggggagaga agctcgcctc
     1981 cgacctggtc gctgccgtgg gtgacgcggt cgcgctgctc cacctgaacg atgcgaagaa
     2041 cgagctcggg agccatcgcg atgggcatcg caaggtgggg gagggatgca tctccatcga
     2101 ggcgtggccg gggcttttcg gaggtctgcc cggggtgccc gtggtcatgg agacgcccta
     2161 cgagacgccc catgccgacc tggaggagat cctcctcgtc aagaaattgg ctggtgggtt
     2221 gcgcaaagcc tagacaggaa gatagaatct aggatgtgaa gactctagag ataagcccgg
     2281 agcggcacaa aggaga
//
LOCUS       DBNGVD010000538         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4148957, whole genome shotgun sequence.
ACCESSION   DBNGVD010000538 DBNGVD010000000
VERSION     DBNGVD010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4148957"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC16CM_14515"
     CDS             <1..583
                     /locus_tag="AC16CM_14515"
                     /inference="COORDINATES: protein motif:HMM:NF044669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141094.1"
                     /translation="VEREPEQEVPEPEEPPREEPVRSEPDGGNEPGIDLASGWVGIMD
                     ELKRRRQALTAAVYGEARVEGFDGRVLRLVYPEEQSFHVGMARDRTHTDKLLSVLEER
                     LGSRPHLEVTAAGGESVAPVAEEPPPPAMEPPDPGPAPEPVPDFGEAPPETGRPDEVS
                     GAQDRGPAPRATADGGVIQNELEVFEIARELES"
     gene            complement(645..1145)
                     /locus_tag="AC16CM_14520"
     CDS             complement(645..1145)
                     /locus_tag="AC16CM_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3141095.1"
                     /translation="MVLICGQQGEAATAGGEVATCGGGSIYLSAKERETFKRHNAIRG
                     RQDLPRFCLHPALQKAARHHSEDMVKRDYFSHDTMGRNEDFSERIESFGYTSYSTLAE
                     NIAYGSGSDGSPSRIMRAWMRSDGHRRNILDPRLRQIGIGVFVGNWKGREGTSMYTAD
                     FGARQR"
     regulatory      complement(1300..1377)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1588..2004
                     /locus_tag="AC16CM_14525"
     CDS             1588..2004
                     /locus_tag="AC16CM_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141096.1"
                     /translation="MDSHDRTTDLDFEILDLLRHGPRSAYRLAGTLSHKGEIVDTETV
                     RSRLVTLASRSLVVGTPSGLADDPEVPRADPWRITGEGRRLVEPEEGVASAPYSREVG
                     GGDGGGRDAGFWVLVVIALVTAVCAAYALLIATGVL"
BASE COUNT          358 a          668 c          702 g          348 t
ORIGIN      
        1 cgtagagcga gagcccgagc aggaggtccc cgagccggaa gagccgcctc gtgaggaacc
       61 cgtccggtca gagcccgacg gcgggaatga gcccgggatc gacctcgcct cggggtgggt
      121 tgggatcatg gacgagctca agcgccgcag gcaggcgctg acggccgccg tctacggcga
      181 ggcgcgggtc gaagggttcg acgggcgggt gctgcggctg gtctaccccg aggagcaaag
      241 cttccacgtc gggatggcga gggacagaac ccacaccgac aagctcctct cggttctcga
      301 agagcgactc ggttccagac cgcacctgga ggtcacggcc gcgggagggg aatcggtggc
      361 ccccgtcgcc gaagaaccac ctccccccgc gatggagcca cccgaccccg ggccagcccc
      421 cgagccggta ccagatttcg gagaggctcc tcccgagacg ggccgtccgg acgaggtctc
      481 cggggcacag gaccgcggtc cggcgccgcg ggcaacggcg gatggcggcg tgatccagaa
      541 cgagctcgag gtcttcgaga tagcacgcga gctggagagt taggccacgg gaggatcagg
      601 gaccacccgg tcgtgtgccc ggattcctac cccatgatcc tggttcagcg ctgtctggcg
      661 ccgaagtcgg ccgtgtacat gcttgtgccc tcgcggccct tccagttacc cacgaagaca
      721 ccgatgccta tctgacgaag tcgagggtcg aggatgttac gacggtgacc gtcgctacgc
      781 atccaggcgc gcattatgcg gctggggcta ccgtcggatc ctgaaccgta tgctatgttt
      841 tcggccagcg tagagtagct ggtgtagccg aaactctcga tgcgttccga gaagtcttcg
      901 ttgcggccca tggtatcgtg cgagaagtag tcgcgcttga ccatgtcctc cgagtgatgc
      961 cgtgccgcct tctgcaacgc tggatgcagg cagaacctcg gtagatcttg ccgcccgcgg
     1021 attgcgttgt gccgcttgaa ggtctccctc tcttttgcgg acagatagat gcttcccccg
     1081 ccgcacgtgg caacctcacc ccccgccgtt gccgcttcac cttgctggcc acagatcagc
     1141 accatagaag cgaccgcgag aaacaaggac atagccacga caacgaggtg tttgccggca
     1201 tggccgtcgc ttggagcaag attcgggatg ccagcggccc catcgggcgc ggcactaccc
     1261 acggtgggtg gcatgacaga actcatggag gagccccctt tcggtagccc ggcgacctct
     1321 gcccggaggc ccgtggcttt gcgtccccgc ctcgcgacgg gtttgccttt tcgttgatgg
     1381 ctacgtacca cactaatgtg tctgctcgcg ccgaaagata cgtcctgacc cgaaccttct
     1441 ccgtccacgg aaaatgccgg gaagccagag agcagcaccg ggtcagcgag gtccaggaac
     1501 gtttacgagg tagggtggag cgggccagta ggctcaaaaa taccggccgg gcggttgcgg
     1561 aacctccgtc cgtgtcatac tgccggtatg gactcccacg atcgcactac ggacctcgac
     1621 ttcgagatcc tcgacctgct gcgtcacggc ccgaggagcg cttaccggct ggccggcacg
     1681 ctctctcaca agggggagat agtcgacacc gagaccgtgc gctcgcgtct cgttacgctg
     1741 gcgtcgcgta gcctcgtcgt tggcacgccc tccggcctgg cggacgaccc ggaggtgcca
     1801 agggccgacc cgtggaggat aaccggggag ggccgacgtc tcgtggagcc ggaggaaggg
     1861 gtggcgagcg ccccctacag tcgggaggtc ggtggggggg atggtggcgg ccgcgacgcc
     1921 ggtttctggg ttctggtggt gatcgccctc gtgacggcgg tgtgcgccgc gtacgcgctc
     1981 ctcatcgcca ccggtgtcct gtagccgccg aagcccggcc aggtcggccg cctccggaca
     2041 aggccgttgc ccctgtaaga tcggtcggaa acctcc
//
LOCUS       DBNGVD010000539         5493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_140232, whole genome shotgun sequence.
ACCESSION   DBNGVD010000539 DBNGVD010000000
VERSION     DBNGVD010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_140232"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16CM_14530"
     CDS             <1..197
                     /locus_tag="AC16CM_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3141097.1"
                     /translation="VEQIREQIGATTLAYLSTEAMVAATRQPKDRLCRACFDGDYPIT
                     GTSNKFALEGQTHFVPTGGG"
     gene            194..601
                     /locus_tag="AC16CM_14535"
     CDS             194..601
                     /locus_tag="AC16CM_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3141098.1"
                     /translation="MSVPQPANHGSQETRTVLAWHEALNSGDVERLLALSHPDVEVGG
                     PRGSAHGTRILREWMDRADIRLEPGRTFDRAGTVVVEQGAEWRSADPGNVQTVASVFV
                     VSDGLVTSVVRYPELASALRAADLDEPHEKSSG"
     gene            647..1126
                     /locus_tag="AC16CM_14540"
     CDS             647..1126
                     /locus_tag="AC16CM_14540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141099.1"
                     /translation="MQTAEPLRIRRYEPGDRGAVRRLHDDALNEVGAHLGSGPWDDDL
                     DEIEGVYLDNGGEFLVGVVDGEVVAMGALMRISPERAELKRMRVRPGLQGRGYGQALL
                     DALHHRAGELGYSRLRLDTTVQQRVAQHLYLKNGYNEAGRGVVGPFDCIFYEREALG"
     gene            1119..2138
                     /gene="purM"
                     /locus_tag="AC16CM_14545"
     CDS             1119..2138
                     /gene="purM"
                     /locus_tag="AC16CM_14545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3141100.1"
                     /translation="MAEKGGYETAGVSIERGERATELMRSAVERTHGQEVLGAPGGFA
                     GLYALAGYEDPVLASTIDGVGTKVLVAKEVGRYDTLGTDIVNHCANDVLATGARPLFF
                     LDYVGSGRLDPEAVAGIVGGAAEACGRLGMALVGGETAEMPGLYDEGDFEIVGVCFGA
                     CERGEVVGGERVRPGDAVIGLASSGLHTNGYTLARKVLADAGISYANRVEGLGRPVGE
                     VYLEPHRSYVREVEALREAVEVRGMAHITGGGLGGNLPRSLGGLGARLDPGSWDEPAV
                     FGLIRSLGGVPEEEMRKVFNLGVGFCAVVSPAEVETGLDALRGAGCGAWKIGEVTQTA
                     GVEFA"
     gene            2131..2712
                     /gene="purN"
                     /locus_tag="AC16CM_14550"
     CDS             2131..2712
                     /gene="purN"
                     /locus_tag="AC16CM_14550"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3141101.1"
                     /translation="MPERLPEGSRFAVLASGSGTNLQALLDAYPENLVVVAGDREEAF
                     AFVRARRAGVPVEHVDPAGFDVKEDYDEELANRVAAHDVGLVVGAGYMRILTPVFLER
                     FPAVLNVHPSLLPDFRGLRAVERALEAGVEETGVTVHFMVEEVDAGPIVAQERVEVEA
                     GDTEATLLERMHPMEHRLLVGAVADYFLGRVEV"
     gene            2709..4259
                     /gene="purH"
                     /locus_tag="AC16CM_14555"
     CDS             2709..4259
                     /gene="purH"
                     /locus_tag="AC16CM_14555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3141102.1"
                     /translation="MKRRALVSVSDKRGVEDFARRLSGMGFEIISTGGTARALSEAGV
                     EVVPVSEVTGAPEILGGRVKTLHPKIHGGILADLGDPDHVQQLVKEDIGPIDLVCINL
                     YPFEEAIAGGAREREAIEQIDIGGPAMLRAAAKNFHSVAVVPSSAFYEEVLNALEGEG
                     QVPEDMRRRLALAAFRRTARYDAAISGWLDEQAGGEVESSGPEKFPERRTIEYERVSS
                     LRYGENPHQEAAYYAEEGADHLLSGVEILQGREISFNNLYDLEAARTLLADLTELGDA
                     AVIVKHANPCGAAVGVSLTDAYRKALASDPQSAFGGIVALGREVDGALAAEISSVFTE
                     ILVAPGFTEEAREILASKPNMRVLVAGPLTRPRLAAKQVTGGILLQDTDAVEDAAGYK
                     VVTETTPSEQQMQDLLFVWRVARVVKSNAIVLARDGATVGVGAGQMSRVDSSEIAVKK
                     AGEKAQGSVAASDAFFPFADGVEALAEAGISAVIQPGGSVRDDEVIEAANRYGLAMVF
                     TGHRHFFH"
     gene            4404..4475
                     /locus_tag="AC16CM_14560"
     tRNA            4404..4475
                     /locus_tag="AC16CM_14560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4436..4438,aa:Lys,seq:ttt)
     gene            4649..5212
                     /locus_tag="AC16CM_14565"
     CDS             4649..5212
                     /locus_tag="AC16CM_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3141103.1"
                     /translation="MRRVGLVLVTMVVVALVVGSGVALAAVKFGTDGGDVLIGTDSAD
                     QLFGKGGSDFLAGKKQDDILYGGDGFDALHGGAVGWGMASDGHDKLFGGDGPDCMFGG
                     SQDDVLVGGSGNDEMGFYCYEFIFDTGIDLFYGGTGNDFVWSWDYSGDGKAQRDLVFC
                     GSGRDEVLADKLDRLHDCERVQRIGRR"
BASE COUNT          993 a         1649 c         1917 g          934 t
ORIGIN      
        1 cggtggagca gataagggag cagatcgggg ccaccaccct ggcctacctc tcaactgaag
       61 cgatggtggc cgcgacccgg caaccgaagg accgcctctg ccgcgcctgc ttcgacggcg
      121 actaccccat cacaggcacc tcgaacaagt ttgccctgga gggccagacc cacttcgtcc
      181 ccaccggcgg cggatgagcg ttccccaacc tgccaaccac ggtagccagg agacccggac
      241 cgtccttgcc tggcacgaag ccctgaacag cggcgacgtg gaacgcctcc tcgcgctctc
      301 ccaccccgac gtcgaggtcg gtggccccag gggcagcgcc cacggcacac ggatcctgcg
      361 cgagtggatg gaccgcgcgg acatccgcct cgaacccgga cgcaccttcg acagagcagg
      421 cacggtggtc gtggaacagg gggcggagtg gagatccgca gaccccggca acgtccagac
      481 cgtggcctca gtgttcgtgg tgagcgatgg tctggtgacg agcgtggtgc gctatccgga
      541 actcgcctcg gctcttcgcg cggcagacct cgacgagcca cacgagaaga gttccggctg
      601 aatcatgcga gatgggagcg gggagttcgg gagaaccggg cagcaagtgc agacagccga
      661 accgctgcgc atccgccgct acgagccggg agacagaggc gccgtcaggc gactgcacga
      721 cgacgcgctg aacgaggtgg gggcacacct cgggagtggt ccctgggacg atgacctcga
      781 cgagatcgag ggcgtctacc tcgataatgg cggcgagttc ctcgttggcg tcgtggacgg
      841 ggaggtcgtc gcgatgggag ctctgatgcg gatctcaccg gagcgggccg agttgaagcg
      901 tatgcgggta aggccggggc tgcagggccg gggctacggg caggccctgc tcgacgcctt
      961 gcaccaccgc gcaggcgaac tcggctactc caggctgcgc ctcgacacga ccgtacaaca
     1021 gcgggtcgcc cagcacctct acctcaagaa cggctacaac gaggcgggac gaggcgtcgt
     1081 cggcccgttc gattgcatct tctatgagag ggaggctctt ggctgagaag ggcggctacg
     1141 agaccgccgg cgtctctatc gaacggggag agagggccac ggagctcatg cgctcggccg
     1201 tggagagaac ccacggccag gaagtgctcg gcgcgcccgg tggtttcgca ggcctctacg
     1261 ccctcgccgg ctacgaggac cccgtcctcg cctcgaccat cgacggcgtc ggcaccaagg
     1321 tgctcgtggc caaggaggtc gggcgctacg ataccctcgg cacggatatc gtcaaccact
     1381 gcgccaacga cgtgctcgcc accggcgccc gcccactctt cttcctggac tacgtgggga
     1441 gcggccggct agatcccgag gccgttgccg gtatagtcgg cggtgccgct gaggcttgcg
     1501 gcaggctcgg aatggcgctc gtcggcggcg agaccgcgga gatgccgggg ctttacgatg
     1561 agggcgactt cgagatcgtc ggcgtctgct tcggggcgtg cgagcggggc gaggtggtag
     1621 gcggcgagag ggtgcggccc ggggacgccg tgatcggcct ggcctcgagc gggctgcaca
     1681 ccaacggcta cacgctggcc cgcaaggtgc tcgccgacgc cggcatctcc tacgcgaaca
     1741 gggtagaggg cctgggacgt cccgtcggcg aggtctacct ggaaccgcac cggtcctacg
     1801 tgcgggaggt agaggcgcta cgagaggcgg tcgaggtcag ggggatggcg cacatcacgg
     1861 gcggagggct cgggggaaac ctgccgcgct cactcggcgg gctcggagca cggctagatc
     1921 ccggatcctg ggatgagcct gcggtcttcg ggctcattcg ttcgctcggt ggggtgccgg
     1981 aggaagagat gcgcaaggta ttcaacctcg gcgtcggctt ctgcgcggtc gtgtcgccgg
     2041 cagaggtgga gaccgggctg gatgcactgc gcggggctgg ttgcggggct tggaagatcg
     2101 gggaggtcac gcagacggct ggggtcgagt ttgcctgagc ggctgcccga gggctcgcgc
     2161 ttcgcggtgc tcgcctcggg ctccgggacg aacctgcagg ccctgctcga cgcctatccc
     2221 gagaacctgg tggtcgtcgc cggggacagg gaagaggcat tcgccttcgt tcgggcgcgg
     2281 cgtgccggcg tgcccgtcga gcacgtggat cccgccggct tcgacgtcaa ggaagactac
     2341 gacgaggaac tcgcgaacag ggtcgccgca cacgacgtgg ggctcgtggt gggggcaggc
     2401 tacatgcgga tactgacgcc ggtcttcctg gagcgtttcc ctgccgtgct caacgtccac
     2461 ccctcgttgc tgcccgactt ccgtgggctc cgggctgtgg agcgtgccct cgaggctggg
     2521 gtcgaggaga cgggcgtgac ggttcacttc atggtcgagg aggtggatgc cgggcccatc
     2581 gtggcccagg aaagggtaga ggtcgaggca ggtgacacgg aggcgacgtt gttggagcgg
     2641 atgcatccca tggagcacag gttgctcgtc ggggcggtcg ctgactattt tcttgggagg
     2701 gtggaggtat gaaacggagg gctctggtct cggtctcgga caagcggggg gtcgaggatt
     2761 tcgcacgacg tctctcgggg atggggttcg agatcatctc cacaggcggt accgcaaggg
     2821 cgctctcgga ggccggggtc gaggtggtcc ccgtctccga ggtgaccggc gcgcccgaga
     2881 tccttggcgg ccgggtcaag acgctgcacc ccaagatcca cggcggcatc ctggccgacc
     2941 ttggagaccc cgaccacgtc cagcagctcg ttaaagagga tatcggaccc atagatctgg
     3001 tctgcataaa cctctacccg ttcgaggagg ccatagcagg cggcgccagg gagagggagg
     3061 ccatcgagca gatagacatc ggcggtcctg ccatgctgcg ggccgcggcc aagaacttcc
     3121 actccgtggc ggtggtcccc tcatcagcct tttacgagga ggtactgaac gccctggagg
     3181 gtgagggaca ggtccccgaa gacatgcggc ggcgtctggc gctcgccgcg ttccggcgca
     3241 cggcccgcta cgacgcggcg atctcgggct ggctcgatga gcaggccggg ggagaggtag
     3301 agtcttcggg gccggagaaa ttccccgagc gccgcaccat cgagtacgag cgggtctcca
     3361 gcctgcgtta cggggagaac ccgcatcagg aggcggctta ctacgcggaa gagggcgccg
     3421 atcacctgct cagtggcgtt gagatcttgc aggggcggga gatctccttc aacaacctct
     3481 acgacctgga agcggcccgc accctcctcg ccgaccttac cgagctcggg gatgctgccg
     3541 ttatcgtcaa gcacgccaac ccctgcggcg ccgcggtagg cgtctccctg accgacgcct
     3601 accgcaaggc cctggcctcc gatccgcagt ccgccttcgg gggtattgtg gcgcttggtc
     3661 gtgaagtaga cggcgcgctg gcggccgaga tctccagcgt gttcacggag atcctggtcg
     3721 cccccggctt caccgaagaa gcgagagaga tcctggcctc caagccgaac atgcgcgttc
     3781 tcgtagccgg cccgctgacc cgtccccggc tcgcggccaa acaggtgaca ggcggcatac
     3841 tgctgcagga caccgacgcc gtagaagacg ccgccggata caaggtggtc accgaaacaa
     3901 caccttcaga gcaacagatg caagacctgc tcttcgtctg gcgcgtggcc cgcgtggtca
     3961 agagcaacgc catagtcctg gccagggacg gtgccacggt cggcgtcggg gctggtcaga
     4021 tgagccgggt cgactcctcg gagatagccg taaagaaggc cggcgagaag gcacaaggct
     4081 ccgtcgcagc cagcgacgcc ttcttcccct tcgccgacgg cgtggaggcc ctggccgaag
     4141 ccggtataag cgcggtcatc cagccaggag gctccgtccg cgacgacgaa gtcatagaag
     4201 ccgccaaccg ttacggcctc gcgatggttt tcaccggtca ccgacatttc ttccattaga
     4261 gcgaacaaca gaggatcgac gccccatcca ttcgtgccat ccgccctcag cagatccaat
     4321 agctgaaagc taacagctga aggctgacag ctagcataag gtcgaagaag cctctggtat
     4381 cgggtaaact ccagacggag cctgggccag tagctcagtg gtagagcagg ggacttttaa
     4441 tcctcgtgtc gtaggttcga tccctacctg gctcaccccg tcgtcggcgt cgagtggcag
     4501 gtatcagcca tggtgctatc tgtcaaaatc ccggaggcgg gacccaccag gcgcgtaagc
     4561 ccctttcctc tgtaagactt gacaacattt gtcgcaatca ctagacttac accgtaagga
     4621 aaacggtggt caagggtagg tggagaccat gaggcgtgtg ggcttggttc tggtgaccat
     4681 ggtggttgtg gcgctcgtcg taggcagtgg cgttgcgctc gcagcggtga agttcggtac
     4741 ggatgggggc gatgtcctga ttggcaccga cagcgcggac caactgttcg gcaaaggtgg
     4801 cagcgacttc cttgctggga agaagcaaga cgatatcctc tacggcggcg acggtttcga
     4861 cgcactccac ggtggtgctg tgggctgggg gatggcctcc gacggtcacg acaagctctt
     4921 cggtggggat ggacccgatt gcatgttcgg cggctcgcaa gacgacgttc tcgtaggcgg
     4981 ttcgggcaac gacgagatgg ggttttactg ctacgagttc atcttcgaca cgggcataga
     5041 cctcttctat ggtggtacag gtaacgactt cgtctggtct tgggattact ctggcgacgg
     5101 caaggcacag cgagacctcg ttttttgtgg cagtggccga gacgaggttc tggcagacaa
     5161 gctcgaccgg ctccacgact gcgaaagagt tcagcgaatc ggacgtagat agcaggcggg
     5221 tgcaccaagc ttggtatgaa ctttagaaac ccatccttca cacgtatcgg cctcatccgg
     5281 ttgggtgggt tggtaactat tgtatgcggc cttgctgcga ccacactagg gttgctcgct
     5341 tgaataggcg ttgtacgagg attccgacca aggtgtggtc tcggtagcta gagccggtgc
     5401 ccgtagtggg gtgctgggaa agggaaagta ggatgcgggc accggctacg gctcttcata
     5461 cagcatatat tcgtgcgagt gaagcctttc gga
//
LOCUS       DBNGVD010000540         1875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3056540, whole genome shotgun sequence.
ACCESSION   DBNGVD010000540 DBNGVD010000000
VERSION     DBNGVD010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3056540"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="trxB"
                     /locus_tag="AC16CM_14570"
     CDS             <1..881
                     /gene="trxB"
                     /locus_tag="AC16CM_14570"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3141104.1"
                     /translation="GQLMLTSDVENYPGYRDGVMGPEMMDDFEAQAARFGAEMRPDNV
                     ERVDFSERPFKLWPEDSEADEPVLARAVIIATGAKAKWLGLESEQQLMGKGVSGCATC
                     DAFFYRGKKVAVVGGGDTAMEEAGVLAKFADEVFLIHRRDEFRASKIMLQRAENNPKI
                     TILTNTVVDNILGDDSVEGVRLRNTETGEEQTIELSGFFTAIGHAPATSLFEGQVEMD
                     EAGYVLQKEHTMTSVPGVFAAGDVSDTRYRQAVTAAGDGCRAAIDSERWLEERGEAEE
                     AEDPAVWTAEKDTANL"
     gene            1056..1514
                     /locus_tag="AC16CM_14575"
     CDS             1056..1514
                     /locus_tag="AC16CM_14575"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3141105.1"
                     /translation="MHIEVETRNVDGRVTALGTAEQFTLVVDRPTEGGGEGLGFNGGQ
                     LLYLAVAGCISNDLFREARTDGIGLESVRVKVGGDFAGEPSVSEEILYEVELEGDAPE
                     ERLQALVDRVDEIAEIPNSLRQGTRVRLARSSLTGNQGDPSDCVMLVRRA"
     gene            complement(1541..>1875)
                     /locus_tag="AC16CM_14580"
     CDS             complement(1541..>1875)
                     /locus_tag="AC16CM_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3141106.1"
                     /translation="TKNASALATRPALAGRYRDALSALGTDRDRGRLGVMTRGDTVGG
                     LSAAFAEAGLAVEQWYGVRVFTDHLEDSTPDEHLPEILELEWEGGKRDPYRSVGRLIH
                     VLGRKKPV"
BASE COUNT          346 a          602 c          650 g          277 t
ORIGIN      
        1 gcgggcagct gatgcttacc tccgacgtgg agaactaccc gggctatcgc gacggcgtga
       61 tgggccccga gatgatggac gacttcgagg cccaggccgc ccgtttcggg gccgagatgc
      121 gcccggacaa cgtggagcgg gtggacttct cggagcgacc cttcaagctg tggcccgagg
      181 atagcgaagc tgacgagccc gtcctggcca gggccgtgat catagctacc ggcgccaagg
      241 ccaagtggct tggcctcgag agcgagcagc agctcatggg caagggcgtg agcgggtgcg
      301 ccacctgcga cgccttcttc tacaggggca agaaggtcgc cgtcgtcggc gggggagaca
      361 cggccatgga agaggccggt gtcctcgcca agtttgccga tgaggtcttc ctcatacacc
      421 gtcgcgacga gttcagggcg tccaagatca tgctccagag ggccgagaac aaccccaaga
      481 tcaccatcct gaccaacacg gtcgtggata acatcctggg cgatgactcg gtggagggcg
      541 ttcgcctcag gaacaccgaa accggcgagg agcagaccat agagctgagc ggcttcttca
      601 cggccatcgg ccacgccccc gcgacgagcc tcttcgaggg ccaggtcgag atggacgagg
      661 cgggctacgt cctgcagaag gagcacacca tgaccagcgt ccccggcgtg ttcgccgctg
      721 gtgacgtctc cgacacgcgc taccgtcagg ccgtcacggc agccggcgac ggctgccgcg
      781 ccgccatcga cagcgagcgc tggctcgagg agcggggcga ggccgaggag gcggaagacc
      841 ccgcggtatg gaccgccgag aaggacacgg ccaacctgta gagacgggtc tcaaaccagc
      901 ccaccacaac ccggacccgc ccggacagac gacgcccccg gcaacgacgc cgggggcgtc
      961 ctcgcgagga acgcctggtc cgccgacgcg ccaccgtgca ggtttagcta ccatgtagcc
     1021 gccgggggcg tagatattgc ggaaggcgtg ggtatatgca catcgaggtg gagaccagga
     1081 acgtcgatgg acgggttacc gcgctcggca cggccgaaca gttcacgctg gtggtggacc
     1141 gtcccaccga gggcgggggc gaggggctcg gcttcaacgg cgggcaactg ctgtacctcg
     1201 cggtcgccgg gtgtatctcc aacgacctct tccgcgaagc caggacggac ggtatcgggc
     1261 tcgagagcgt cagggtaaag gtgggtggag acttcgcggg cgagccgtcg gtctcggagg
     1321 agatcctcta cgaggtcgag ctggagggcg acgccccgga agagaggctc caggccctgg
     1381 tggacagggt ggatgagata gccgaaatcc caaactcgct ccggcaggga acgcgggtac
     1441 gcctggcaag gtcgagcctg acggggaatc aaggcgatcc gtccgattgc gtgatgctgg
     1501 ttcgccgcgc ataacacgcg aaccatccct cgatggcccc ctacacaggc ttcttgcgtc
     1561 ctagcacgtg gatgaggcgg ccaacggatc tgtaaggatc cctcttgccg ccctcccact
     1621 ccagctccag gatctcgggc aggtgctcgt ctggcgtgct gtcttcgaga tggtccgtga
     1681 agacccgcac cccgtaccac tgctcgaccg cgaggccggc ttcggcgaaa gccgcggaca
     1741 gtcctccgac cgtatcgccg cgcgtcatca ccccgagccg tcccctgtcc ctgtccgttc
     1801 cgagcgcgga gagcgcgtcc cggtagcgtc ctgcgagggc gggccgcgtg gcaagggccg
     1861 aggcgttctt tgtca
//
LOCUS       DBNGVD010000541         2824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2873206, whole genome shotgun sequence.
ACCESSION   DBNGVD010000541 DBNGVD010000000
VERSION     DBNGVD010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2873206"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC16CM_14585"
     CDS             <1..652
                     /locus_tag="AC16CM_14585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3141107.1"
                     /translation="RTTPIGRAERRSHGLSALLVDLREAEGLTVRPRRMMMNNATNEL
                     VFDGLRVPAENLIGEEGRGFRYILDGMNAERILIAAECIGDGRWFVEKATGRAVEREV
                     FGRPIGENQGIQFPIARAYANIEAATLMVEKAAALFDRGEPCGAEANLAKLLAADASW
                     EAANVAVQTFGGYGFDADYDVERKFRETRLYQVAPISTNLILSYIAEHVLGLPRSF"
     gene            652..1812
                     /locus_tag="AC16CM_14590"
     CDS             652..1812
                     /locus_tag="AC16CM_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3141108.1"
                     /translation="MTLPLEGITVVSLEQAVAAPFATRQLADLGARVIKVERPGVGDF
                     ARSYDAVVEGLSSHFVWINRSKESIALNLKQEAARGVLSSLLETADVFVQNLAPGATG
                     RLGFGTAALRERHPELIVCDVSGYGASGPYRDKKAYDLLVQCEAALVSVTGTQETPSK
                     AGIAVADIAAGMYAYSGILAALFRRERTGEGAALEVSLFEALAEWMGFPAYYAMYGEK
                     EPPRSGASHASIAPYGPFACAGDEVVFFGIQNEREWEKFCGVVLGRPELTGDERFSTN
                     SDRLDNRDDLDGEIERIFGSLTAEEVIEKLEEAGIANARLRSVREFLDHPQLEARARW
                     RKVDSPSGPLRALLPPGLPTDIEPVMAPIPEVGEHTTAILAELGYDADVLEG"
     gene            complement(1988..>2824)
                     /locus_tag="AC16CM_14595"
     CDS             complement(1988..>2824)
                     /locus_tag="AC16CM_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3141109.1"
                     /translation="VRDLSRPNGHFEATLESGGRITADAVVAAPGISHFTVVPEWVEK
                     SLPPERWGHTCNLVDFEELRGKRVMIVGGRQSAFEWAALLADGEAAEVNVVYRHDTPR
                     FTTSDWHFTDELIDLTATVPGWFRSLPTEEREAIARRFWAEGRLKLEPWLTPRLDNPS
                     VHRWPQRSVVSCQDDGVDIEVRLSDGVQLAVDFVILATGYKPDMRAVPYLSGVIDDME
                     LADGFPVLDDNFQTSVPGLFVTGFPATRDFGPFFGFVRGCPVAAALTTAGLEKALGHT
                     HA"
BASE COUNT          480 a          956 c          924 g          464 t
ORIGIN      
        1 ccgcaccacg cccatcggga gggccgagag acgctcccac ggcctctccg ccctcctcgt
       61 cgaccttcgg gaagcggagg gcctcaccgt gaggccccgc cggatgatga tgaacaacgc
      121 cacgaacgag ctcgtcttcg acggcctcag ggtacccgcc gagaacctca tcggcgagga
      181 gggccgtggg ttccgctaca ttctcgacgg catgaacgcg gagcgcatcc tgatcgccgc
      241 cgagtgcatt ggcgacggca ggtggttcgt ggagaaggct acgggtcggg ccgtagagcg
      301 cgaggtgttc gggcggccta tcggagagaa ccagggcatc cagtttccga tcgcgagggc
      361 ctacgcaaac atagaggccg ccacgctgat ggttgagaag gcggccgccc tcttcgaccg
      421 gggggaaccc tgcggagccg aggcgaacct ggccaagctt ctcgccgctg acgcctcctg
      481 ggaggccgca aacgtggccg tgcagacctt cgggggctac ggcttcgacg ccgactacga
      541 cgtggagcgc aagttccgcg agacgcgtct ctaccaggtg gcccccatct cgaccaacct
      601 gatcctctcc tacatcgcag agcacgtgct cggcctcccg aggtcgttct gatgacgctg
      661 cccctcgagg gcataaccgt ggtctccctg gagcaggcgg tcgccgcgcc cttcgccacg
      721 cgccagctcg ccgacctggg agcgcgggtc atcaaggtgg agcgtccggg ggtcggggat
      781 ttcgcccgct cctacgacgc cgtcgtggag gggctctcca gccacttcgt ctggatcaac
      841 cgctccaaag agtcgatcgc cctgaacctg aagcaggagg ccgcccgcgg cgtcctgagc
      901 agcctgctgg agaccgccga cgtcttcgtc cagaacctgg ctcccggcgc gacggggcgt
      961 ctcggcttcg ggacggcagc cctgcgcgag cgccaccccg aactcatcgt gtgcgacgtc
     1021 tctggttacg gtgcctccgg cccttacagg gacaagaaag cctacgacct tctcgtccag
     1081 tgcgaggccg ccctcgtctc cgtaacgggc acgcaggaga cgccatccaa ggcaggcatc
     1141 gccgtcgcgg atatagcggc tggcatgtac gcctactccg gcatcctggc ggcgctgttc
     1201 aggcgcgaga ggacaggtga gggcgcggcc ctggaggtct cgctcttcga ggcgctcgcc
     1261 gaatggatgg gcttccccgc ctattacgcc atgtacggag agaaggagcc gccgcgtagc
     1321 ggcgcgagcc acgcctccat agcaccctac gggcccttcg cgtgcgccgg cgacgaggta
     1381 gtcttcttcg gcatccagaa cgagcgggag tgggagaagt tctgtggggt cgttctgggg
     1441 cgacccgagc tgaccgggga cgagcgcttc tcaaccaact cggacaggtt ggacaacaga
     1501 gacgacctag atggggagat cgaacggatc ttcggcagcc tcacggccga ggaggttatc
     1561 gagaaactcg aagaggccgg tatagccaac gccaggctca ggtccgttcg agagttcctt
     1621 gaccacccac agctagaggc ccgcgcccgc tggcgcaaag tagattcccc ttccggcccc
     1681 ctgcgcgccc tcctaccgcc cggcttgccc acggacatcg agccggttat ggccccgatc
     1741 cccgaggtgg gagagcatac gaccgccata ctcgccgagc taggctacga cgcggatgtg
     1801 ctggagggat agacgaggtt tcggttcgtc tacccgtgtt tagatccagc acactagggt
     1861 aagtctcgta ggcaagcccg gtggcccaca gagtgcgatg ctttgtgttt ccccctccaa
     1921 agccgccggg tgatcactct ctaggcgcaa ccctctctgc ttgctgatcg ctaaccgcgc
     1981 cccgccatca ggcgtgggtg tgtccgagcg ccttctcgag gcctgctgtg gttagcgcgg
     2041 cggccacggg gcatccgcgc acgaagccga agaacgggcc gaagtcccgc gtggccggga
     2101 aacccgtaac gaaaagcccg ggcacgctgg tctgaaagtt gtcgtccagt accgggaacc
     2161 cgtccgcgag ctccatgtcg tcgatcaccc cggagaggta gggcacggcc cgcatgtcgg
     2221 gtttgtatcc ggtggccagg atcacgaagt ccacagcgag ctgcacgccg tccgagagcc
     2281 ggacttcgat gtccacgccg tcgtcctggc acgacaccac cgatcgttgc ggccagcgat
     2341 ggacggacgg gttgtccagg cggggggtga gccacggttc cagcttcagc ctgccctccg
     2401 cccagaacct cctggctatt gcctcacgct cctcggtggg cagagacctg aaccaccctg
     2461 gcacggtggc cgtgaggtct atcagctcgt cggtgaaatg ccagtcggag gtggtgaacc
     2521 gcggcgtgtc gtgcctgtag accacgttga cttccgcggc ctccccgtcg gccagcaggg
     2581 cggcccattc gaaggcgctc tggcgcccac ccacgatcat cacccgcttc ccgcgcagtt
     2641 cctcgaagtc gaccaggttg caggtatgcc cccagcgctc cggcgggagg ctcttctcga
     2701 cccactcggg gacgaccgtg aaatgggaga tgccgggtgc cgcgaccacg gcgtcggccg
     2761 tgatgcgccc gccgctctcc agcgtcgcct cgaagtgccc gtttggcctg gagaggtccc
     2821 gcac
//
LOCUS       DBNGVD010000542         2621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3238779, whole genome shotgun sequence.
ACCESSION   DBNGVD010000542 DBNGVD010000000
VERSION     DBNGVD010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3238779"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="AC16CM_14600"
     CDS             complement(<1..1358)
                     /locus_tag="AC16CM_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR3141110.1"
                     /translation="MASLVGCVVLAGWTLDAGTLGDFLPGLVAMNPVSAVGLVLAGTS
                     AWLLRDGGADRRARRVARVFALAVVFIGLLRLVQVLLGLEPGVDRILFPGRLASEAAS
                     TGFSNRMAFDTALGFVLIGVSLFCLDGQNRLVRRLSQQAALVVLVLSVLAFAGYLYGV
                     ANLYGNTSYIPIASLTFVVLSAGILCSRPRSGLMAIVTSRNLGGMMARRLLPAAVLVP
                     LVLGWLRLRGQQIGLYGTELGVSLITVANMFVVAALVWWSARLLYRIDAERRRAGEEM
                     RAARQRAEEAREEAERANQAKSEFLANMSHEIRTPMNGVIGMTDLLLDTSLDDEQREY
                     AETVRFSGQSLLAILNDILDFSKIEAGSLDLETIDFELRGEVEEVAALLAGRAHEKGL
                     ELASFVEPGTPTAVRGDPFRLRQVLTNVLGNAIKFTERGEVTLRAELAEDAPDAATVL
                     FEV"
     gene            1776..2435
                     /locus_tag="AC16CM_14605"
     CDS             1776..2435
                     /locus_tag="AC16CM_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141111.1"
                     /translation="MPTSQDVIEKIEATFAGNAFPGARFLQGSFEGCEPYDEVGPFEK
                     LEDWREIEAPFLDGHANALSFFSEAGFRYFLPAYLISDLRGLLYTADPLFHLTHGFSD
                     RTTEVPTGDRTLAIKHGRSAFINPRRYGAMTSHDYARYRLSIFTREEAGAIVGYLEFK
                     RDADPDVIDKQAINEALHSFWLERARTAPLAQDLGQHIAEQEEYLATIRPDIEDSTDR
                     P"
BASE COUNT          495 a          884 c          826 g          416 t
ORIGIN      
        1 acctcgaaga ggaccgtggc cgcgtctggg gcgtcttcgg cgagctcggc ccgcagggta
       61 acctcgcccc gctcggtgaa cttgatcgcg tttccgagca cgttggtcag gacctgacga
      121 aggcggaagg gatcgcccct gacggcggtc ggcgtgcccg gctcgacgaa gctggccagc
      181 tccaggccct tctcgtgggc tcgtccggcg agcagggcgg cgacctcctc gacctccccc
      241 ctcagctcga agtctatggt ctccagatcg aggctgccgg cctctatctt ggagaagtca
      301 aggatatcgt tcaggatggc cagcaggctc tggccggaga agcgcaccgt ctcggcgtac
      361 tctcgctgct cgtcgtcgag gctggtgtcg agcaggaggt ccgtcatccc tatcaccccg
      421 ttcatgggcg tacggatctc gtggctcatg ttggccagga actcgctctt ggcctggttg
      481 gccctctcgg cctcttccct tgcctcctcg gccctctgcc tcgcggcccg catctcctcg
      541 ccggccctgc ggcgctcggc gtcgatgcgg tagagcaggc gggcgctcca ccacaccagc
      601 gcggcgacga cgaacatgtt ggccacggtg atgagggaca ctccgagctc cgtgccatag
      661 agccctattt gctggcccct caggcggagc cagccgagca ccagcgggac cagcacggcg
      721 gcgggcagca gtcggcgcgc catcatgccc ccgaggttgc ggctggtgac gatggccatg
      781 aggccgctcc ggggacggga gcaaagaatg ccggcggaga gcacgacgaa agtcagcgac
      841 gcaatgggga tgtaggaggt gttgccgtac aggttggcga cgccgtagag gtagccggcg
      901 aaggccagca ccgagagcac cagtaccacc agggcggctt gctgggagag cctgcggacc
      961 aggcgattct gcccgtccag gcagaagagg gagaccccga tcaggacgaa accaagcgcc
     1021 gtgtcgaagg ccatcctgtt ggagaaaccc gtggaagcgg cctcggacgc cagccttccg
     1081 gggaacagga tccggtcgac gccaggctcc aggccgagca gcacctgaac cagcctcaac
     1141 aggccgataa agaccacggc gagcgcaaac acccgcgcta cccgtcgcgc cctccggtcc
     1201 gcccccccat cccgcagcag ccacgccgac gttccggcca gcaccaggcc caccgccgag
     1261 acggggttca tcgccacgag accgggcagg aagtctccga gagtgccggc atcgagcgtc
     1321 cagccggcga gaaccacgca gccgacgagt gaggccacga tgcttgacgc ccgcgagaaa
     1381 gtcttcagga aagaaacgag accaggaacg gagtcgtctt ccagcccgca ccgtccggtc
     1441 gtgtcgtttc cctgaccgat gatctcgtac tccccttgcc ggctctccct acgtcaaccg
     1501 gtagcaattg tactctaagg gtcaacacca cggcccgcac gggctcagtc accagggatc
     1561 agctcacaag ggagctggca tttcacaccg gatctgcttg gacgatcgcc ctttcgggct
     1621 gctggcgcgc ctgattttcc cgatccgtca ttcccccgga agcgggaatc cacggctaac
     1681 agaagagatg ggctcttacg ccgtagcgtt gccattcacc cggacctggt attagagtgc
     1741 ggaattgccg acaagcggag gcggaggacc ggagcgtgcc aacaagccaa gacgtgatcg
     1801 agaagatcga agccactttc gcgggcaacg cgtttccggg ggcgcgtttc ctgcagggca
     1861 gcttcgaggg gtgcgagcct tacgacgaag tcgggccctt cgaaaaactc gaggactggc
     1921 gggagatcga ggcccctttc ctggacggcc acgcgaacgc gctcagcttc ttctcggagg
     1981 ccggcttcag gtactttctg ccggcgtacc tgatctccga cctgcgtggc ctactctaca
     2041 ccgcagaccc cctgttccac ctgacccacg gcttctccga ccggaccacc gaggtcccca
     2101 ccggagaccg caccctcgcc ataaagcacg gcaggtcagc gttcataaac ccacgacgct
     2161 acggcgccat gacctcccac gactacgccc ggtaccgcct gtcgatcttt accagggagg
     2221 aagcgggcgc catcgtcggc tacctggaat tcaagcgcga cgcggacccc gacgtcatcg
     2281 acaaacaggc catcaacgag gccctccact cgttctggtt ggagagagcg cggacggcgc
     2341 ccctggccca ggatctcggg caacacatag cggagcagga agaatacctg gccaccatac
     2401 gccccgacat cgaggacagc acggatagac cgtgaacttc gaccccgcca ggatgcttct
     2461 cgtgaacggc tgaaggcaac gtctgagcgg cggtcaacga tgcgcagggt attggacaag
     2521 cgggaacgac tcaccggcct ttttgtggtg atcggcgcca ccctcttgtt cgcgcccgtg
     2581 gtctggctct cgcagcgttg gggcgaagcg gtggcccgcg a
//
LOCUS       DBNGVD010000543         4031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_871258, whole genome shotgun sequence.
ACCESSION   DBNGVD010000543 DBNGVD010000000
VERSION     DBNGVD010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_871258"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..637
                     /locus_tag="AC16CM_14610"
     CDS             194..637
                     /locus_tag="AC16CM_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3141112.1"
                     /translation="MVEGGSKEEILRGVHPVLPSLALAFKRMLTTIERESGIGGMKWF
                     LLTVLDRRDGLSQGEFTQEYELEPSRVTRTAQSLEADGLIRRERDVDDNRVMRMYLTE
                     EGSRVLDRLPEINEQLRSRVHSVLSEEEFEELRRMLGLLAEAMKS"
     gene            663..2576
                     /locus_tag="AC16CM_14615"
     CDS             663..2576
                     /locus_tag="AC16CM_14615"
                     /inference="COORDINATES: protein motif:HMM:NF018332.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3141113.1"
                     /translation="MSANATQAGQSGRRGTVLAVAGLMLALFLVALDQTVVGTALPEI
                     IADLDGFEHYAWVTTAYLLASTAMIPVIGKLGDIYGRKWFIIAGVAIFLVASALCGAA
                     WGMTELILFRGLQGLGAGMIFSNIFTSVADIFPDPAQRAKYQGVFFSVFALTSVIGPT
                     LGGWITDNLSWRWVFYINLPLGIFSLFALPVVLHQSMRRVGVKIDFLGAATITASVVS
                     LLLALSWVGEGYDWDATRVVVGFVVAGILFATFVPVELRAAEPIIPLTLFKSRVFSSA
                     AILMFMVGIGMFGIILYTPLFVQGVLGKTATGSGTVLTPLVLSMTAMGIICGQIIARV
                     KRIKPFMIGGAIVMTFGIYLLTTLDVDSTQRTVALYLMITGLGLGPLMPSATLAVQST
                     VQKRLLGVATSSTQFIRSLGSTVGTAVIGSLVTSGYANYLNDNAPAAAPGRLVNALKD
                     PNALVSDEARDALDRIASAFPGGEQVVNQILDAARTGLSNSIHDGFVFTLVAVSFAIV
                     AAVLMKNIRLGDQNEDQPAAPAPGLERDRVLTGITLEYLARRIESANGDSRNLISAAS
                     ALVPNAEGSERERARLAADRVLRPMALKALGTGLGGNGTNNGSAGHSANGSIPNGQAP
                     LGQHEDGPERSSR"
     gene            2576..3031
                     /locus_tag="AC16CM_14620"
     CDS             2576..3031
                     /locus_tag="AC16CM_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141114.1"
                     /translation="MVKYVIGAVGALLGALAGAWLMLAPFALAYQDSGADWADPTFVD
                     FWTGLPLLIVSLAGVILYVSGLVEELRRRGIMRRPYAESAGQPQTSAEAPDNASGAEN
                     NMEQVLMPLVTAMLKDMQERDRGRDEAHRETTNNLQQARNAGEEGSVRS"
     gene            3031..3291
                     /locus_tag="AC16CM_14625"
     CDS             3031..3291
                     /locus_tag="AC16CM_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141115.1"
                     /translation="MAPQARGGLIVTVVGFFVGIWLFLAPFVVEYQKVGQDWIDATRN
                     DLWTGGALIAISALTLLLFAAFALRDAVRAAEQKQVTQAEES"
     gene            3359..3449
                     /locus_tag="AC16CM_14630"
     tRNA            3359..3449
                     /locus_tag="AC16CM_14630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3394..3396,aa:Ser,seq:gga)
     gene            3710..3952
                     /locus_tag="AC16CM_14635"
     CDS             3710..3952
                     /locus_tag="AC16CM_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141116.1"
                     /translation="MTEPPVALASAVGVEPGYFLAGVEESPMLDREALDILRDETTSA
                     IARKSFRLPSPEKRMILNIIDELENARRARDLEATS"
BASE COUNT          749 a         1260 c         1286 g          736 t
ORIGIN      
        1 tccctcccgc gaacgcggga acccacgaat agaccagacc ctcacgggcc ccacaagaac
       61 gacctttggg gccgccgatg accgatcttc gatgacgcgt gaatatcctc acaattgttt
      121 cacttgcaaa ggttgaaact gcaaagaacg gacgtagact tcgcacgata gcaaggcaat
      181 cggaggatgg acgatggtag agggcgggag caaagaagag attttgcggg gcgttcatcc
      241 ggtgctgcct tcgctcgcgc tggcgttcaa gaggatgttg acgaccatag agcgcgagtc
      301 cgggatcggg ggcatgaagt ggttcttgct cacggtgctg gacaggaggg atgggttgag
      361 ccagggcgag ttcacgcagg aatacgagtt ggaaccgtca cgcgtgaccc gtacggcgca
      421 gtcgctcgag gccgacggtt tgatccgcag ggagcgcgac gtggacgaca accgcgtgat
      481 gcggatgtat ctcacggaag agggctccag ggttctggac cggttgcctg agataaacga
      541 gcagcttcgg agcagggttc acagcgttct gagcgaggaa gagttcgagg agttgcggcg
      601 gatgctgggc cttctcgccg aggcaatgaa atcctaggtc cttcatcaga gggaggtcgg
      661 agatgagcgc gaacgcaact caagcaggac agtcggggcg tagggggacc gtgctcgcgg
      721 tggcggggct catgctcgca ctatttctcg tcgcgctcga ccagacggtt gtcgggaccg
      781 cgttgccgga gatcatcgcc gacctcgacg ggttcgagca ttacgcgtgg gtaaccacgg
      841 cgtacctgtt ggcttccacg gccatgatcc cggtcatcgg caagctcggc gacatctacg
      901 gcaggaagtg gttcatcatc gccggggtgg caatctttct cgtcgccagc gcgctctgcg
      961 gggcggcgtg ggggatgacg gagttgatcc tcttccgcgg cctgcagggt cttggggccg
     1021 gcatgatctt ctccaacatc ttcaccagcg tcgccgacat ctttcccgac ccggcccagc
     1081 gggccaagta tcagggcgtc ttcttcagcg tcttcgccct gacgagcgtc atagggccga
     1141 cgcttggtgg ttggatcacg gacaacctct cctggcggtg ggttttctac atcaacttgc
     1201 cgctcggcat cttctccctg ttcgccctgc ccgtggtgct gcaccagagc atgcggcggg
     1261 tgggggtaaa gatcgacttc ctcggggccg ccaccataac agcctcggtc gtctcgctcc
     1321 tgctagcgct ctcatgggtc ggtgagggct acgactggga cgccacccga gtcgtcgtcg
     1381 ggttcgtggt tgccggcatc ctgttcgcca ccttcgtgcc cgtagagctc agggctgccg
     1441 aacccataat tcctctcacg ctcttcaaga gccgcgtctt cagctccgcc gcgatcctca
     1501 tgttcatggt cggcatcggg atgttcggga tcatcctcta caccccgctc ttcgtgcagg
     1561 gggtactcgg caagaccgcg accgggtccg gcaccgtgct taccccgctc gtcctaagca
     1621 tgacggccat gggcatcatc tgcggtcaga tcatagcccg cgtcaagcgg atcaaaccgt
     1681 tcatgatcgg cggtgccatc gtgatgacct tcggcatcta cctgctgacg acgctcgacg
     1741 ttgactccac ccagaggacg gtggccctct acctcatgat caccggcctc ggcctcggcc
     1801 cgctcatgcc gtctgccacc ctcgccgtgc agagcacggt ccagaagagg cttctcggtg
     1861 tggcgacctc ctcaacccag ttcatccggt ccctcggctc gacggtcgga acggccgtga
     1921 tcgggtcgct tgtcacctcc gggtacgcca actacctgaa cgacaacgcg ccggccgcgg
     1981 cgcctggccg gcttgtcaac gccctcaagg atcccaacgc gcttgtcagc gacgaggccc
     2041 gcgacgcgct tgaccgcatc gcttccgcct tccccggcgg agagcaggtc gtaaaccaga
     2101 tcctggacgc ggcccggacg gggctctcga actccatcca cgacggcttc gtgttcaccc
     2161 tcgtagctgt ctccttcgct atcgtggccg ctgttctgat gaagaacata cgcctcggcg
     2221 accagaacga ggaccagccc gccgccccgg ccccaggtct cgaacgggac agggtgttga
     2281 cggggatcac gctcgagtac ctggccaggc gcatcgagag cgccaacggc gactcccgga
     2341 acctgatctc cgcagcttcc gccctggtcc cgaacgctga gggttcggag agggagcggg
     2401 caaggctcgc cgccgaccgg gtactgaggc cgatggccct gaaggccctc ggcaccggcc
     2461 tcggaggcaa cggcacgaac aacggttccg ccggacattc agcgaacggc tccatcccca
     2521 acggtcaggc tccgttgggg caacacgagg atgggccgga aaggagcagc agataatggt
     2581 caagtacgtt attggggcgg tgggagcgtt gctcggcgcc ctcgccgggg cgtggctgat
     2641 gctggcccct ttcgccctcg cctaccagga ctcgggggcc gactgggccg acccgacgtt
     2701 cgtggatttc tggacagggc tcccgctcct gatcgtgtcc cttgcagggg tgatcctcta
     2761 cgtctcgggt ctggtggagg agttgcgtcg ccgcggcatc atgcgccgcc cctacgcaga
     2821 gtccgcagga cagccccaga cttcggccga ggccccggat aacgcttcgg gagccgagaa
     2881 caacatggaa caggtcctga tgccgctcgt aacagcgatg ctcaaggaca tgcaggagcg
     2941 cgaccgtggc cgcgacgaag cccaccggga gaccaccaac aaccttcagc aggcccgcaa
     3001 cgcgggagaa gaagggagcg tgaggagctg atggcaccgc aagcaagggg tggcctgatc
     3061 gtcaccgtcg tagggttctt cgtggggata tggctgttcc tggccccgtt cgtcgtcgag
     3121 taccagaagg tagggcagga ctggatcgac gccaccagga acgacctctg gaccggtgga
     3181 gccctcatcg ccatctccgc cctgaccctg ctacttttcg ccgccttcgc cctgcgtgac
     3241 gcggtgcgcg ccgccgagca gaagcaggtg acccaagcag aggaaagcta ggacgcggat
     3301 cagagttcta acggtagcag cggtttcgct ttttggtaaa ctcccgtccg tcgcttccgg
     3361 agaggtgtcc gagttggccg aaggagcgcg attggaaatc gcgtaggcgg tgttaagccg
     3421 tctcgagggt tcgaatccct ccctctccgc ttttcccacg atttgcaggt aaaacgtagc
     3481 aggtgcatga agagagtaaa tgggctccgg cgttgtgccg gagcccattt actgcaaccg
     3541 tactgcaacc cgtcagccga aagcgtggtc cccggtggtc cttctcggca cggctacgga
     3601 caccgcagac aaagacgaga gcgccgaacg accactgcag gcttcgtgag gctcgcagta
     3661 ccctgcctcc cgatcggaat ccacgtggag ctttgtctcc tgcgagggct tgacggaacc
     3721 gccggtcgca ctggccagcg ccgtcggcgt cgagccgggg tacttcctgg ctggggtaga
     3781 ggaatcaccg atgctcgaca gagaagcatt ggacatactc cgggacgaga cgacgagcgc
     3841 catagcgcgc aagagcttcc gtctgccgag tcccgagaag cggatgatct tgaacatcat
     3901 cgacgagctg gagaacgcgc gccgtgcgcg cgacctagag gctacttcct agctcccggt
     3961 aaggagaacc agaccaagga aagcgaagct agaggcatca ggactcgcct gctgccctca
     4021 ttagtcatag c
//
LOCUS       DBNGVD010000544         4877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4878537, whole genome shotgun sequence.
ACCESSION   DBNGVD010000544 DBNGVD010000000
VERSION     DBNGVD010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4878537"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16CM_14640"
     CDS             complement(<1..275)
                     /locus_tag="AC16CM_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase-like domain-containing protein"
                     /protein_id="HIR3141117.1"
                     /translation="MPDCLRLSVWSGPRNVSTALMYSFRQRPDTVVVDEPLYGHYLST
                     SDAVHPGATEVLKTMDGDGERVVREMILGPCERPIHFFKNMAHHLPGL"
     gene            complement(268..1197)
                     /locus_tag="AC16CM_14645"
     CDS             complement(268..1197)
                     /locus_tag="AC16CM_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3141118.1"
                     /translation="MARGTHEALPDERNEGIFVYINGDFFRREDAKISVFDSGFLVGD
                     GVWEGLRLHDGEFAFLGLHLDRLFEGAKAIHLDIGMTRAEITSALRETIRRNGMEGTS
                     GVHVRLMVTRGDKKTPSQDPRLVVGGPNVVIIAEHKVADPGVRDQGVRLFTSTVRRPP
                     PDTLDQRLNCHSKLHEVVALVQALQAGADEALMLDPTGAVATCNATNFFIVRRGEVWT
                     STGHYSLNGITRRMIIALCRAENIPVSERPFSLTDVYGADEAFVTGTFGGLTPVIEVD
                     GRRIGQGTRGETTARLTDHYAEAIAEATRGQDA"
     gene            complement(1178..1732)
                     /locus_tag="AC16CM_14650"
     CDS             complement(1178..1732)
                     /locus_tag="AC16CM_14650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141119.1"
                     /translation="MLRGERVTLRAVEMEDQEVLWRFWNDLGVELAGGGDPPLPLSFE
                     RQRARFEREEREARRDKTDFMIEVDGRAIGHCGLFNLDVAARHCELGISIGEKDHWGR
                     GYGREAVKLLLDYAFRIRNLRRVWLETHASNERAIRAYRACGFVEEGRMREHVWLAGR
                     YVDNVIMGVMRDEWEGGTVGQGNA"
     gene            complement(1732..2376)
                     /locus_tag="AC16CM_14655"
     CDS             complement(1732..2376)
                     /locus_tag="AC16CM_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141120.1"
                     /translation="MGRYWPSLYTEFAGYQVVVCDGAGEVLAAGHSLPVFWDGTRDGL
                     PAGLDGVLERGVGGGSGGAPTVVSALLAVVPPAHRGRGLSSVVLRAMKTIAAARGLGA
                     LIAPVRPTWKERYPLTPMERYAGWRRDDGLPFDPWLRVHYRLGARFVAVALESMVIAG
                     TVSEWEGWTGMRFPETGPYVVEGALQPVEMDLERDLGVYTEPNVWMRHPVGSGA"
     gene            complement(2545..3795)
                     /gene="lysA"
                     /locus_tag="AC16CM_14660"
     CDS             complement(2545..3795)
                     /gene="lysA"
                     /locus_tag="AC16CM_14660"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3141121.1"
                     /translation="MDDFHYEGGGLRCEDVPLSEIAAGAGTPTYVYSHTALQRAYEEL
                     DEAFSGLDHLVCFAVKANGNLAVLRALASFGAGADIVSGGELYRTMRAGFDPKKVVFA
                     GVGKTERELMAGLGERILLFNVESASELDLLDRICSRFGKRARVALRVNPNVDAETHE
                     HITTGRSRDKFGIPVDEALALAERVEGYRCVELIGVHQHIGSQITKLTPFSESVERSA
                     GLVDELKTRGFDIQYFNIGGGLGIPYKDEETPSPRELVDAIRPTLESTGTKILCEMGR
                     YIAGAAGVLLTRVIYRKKSGEKSFIVADAGMNDLLRPSLYDAHHEVLPVEENGSVANA
                     DLVGPVCESGDYLARDRELPDAAEGEVLVVKDAGAYGFSMASNYNSRPRPAEVMVRGD
                     RWAVVRERENDADLIKGELVPAFL"
     gene            3871..>4877
                     /gene="dapC"
                     /locus_tag="AC16CM_14665"
     CDS             3871..>4877
                     /gene="dapC"
                     /locus_tag="AC16CM_14665"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR3141122.1"
                     /translation="MPNPRATPRANEITTTSPAPPLLLNPILSHQGAYPLLRLDERRK
                     ALEDSGAELFDFGTGDPREPTDERIRQALIEGVPASSRYPTSSGKRELREAFCGWMER
                     RHGVTLDPEREVLPATGSKEAIFHAPLAFLHHTHEQRGVAYGTPGYPVYERGTLFAGG
                     EARPVELKGENGFLLPPEAVDPGETRILWINYPHNPTGATATYEYLERVAEFCREHDL
                     LLFSDECYNDVYSGEPPPSILEVSRERTLAFCSLSKRSGMTGYRSAIMAGDPEIISAL
                     AKLRPSVGVATQSFVQDAATAAWSDDAHVEKRRRIFGQKRALFTAFFDRAGLEYLQTD
                     A"
BASE COUNT          836 a         1758 c         1473 g          810 t
ORIGIN      
        1 aggccgggga ggtggtgggc catattcttg aagaagtgaa tggggcgttc gcacgggccg
       61 aggatcatct cccgcaccac cctctcgccg tccccgtcca tggtcttcag gacctcggtg
      121 gcgcccggat gcaccgcgtc cgaggtgctc aggtagtgtc cgtagagggg ctcgtcgacc
      181 accacggtat ccggcctctg ccggaaggag tacatgagcg ccgtcgagac gttgcgcggg
      241 cccgaccaga ccgaaagcct gaggcagtca ggcatcctgt cctctcgtcg cctcggcgat
      301 tgcctccgcg tagtggtccg tcaggcgggc ggtcgtttcg ccgcgcgtgc cctgcccgat
      361 cctgcgcccg tccacctcga taaccggcgt gaggccgccg aaggtgccgg ttacgaaagc
      421 ctcgtccgcg ccgtacacgt cggtcaggga gaagggccgc tccgagacgg gtatgttctc
      481 tgcccggcag agggctatga tcatgcgccg cgttatgccg ttcagggagt agtggccggt
      541 cgaggtccac acctccccgc gccgcacgat gaagaagttg gtggcgttgc aggtggcgac
      601 agcccccgtg gggtcgagca tcagggcctc gtcggcgccg gcctgtagtg cctgaaccag
      661 ggccacgacc tcgtgcagct tgctgtggca attgagcctc tggtcgagcg tgtctggcgg
      721 cgggcggcgt acggtcgagg taaagagcct gacgccctga tccctcaccc ccgggtcggc
      781 caccttgtgt tcggcgatga tgacgacgtt tgggccaccc accacgaggc gcgggtcctg
      841 cgatggggtc ttcttgtccc cgcgggtaac catcaggcgc acgtgcacgc ctgaggtgcc
      901 ttccatgccg ttgcggcgga tggtctcgcg gagggcagat gtgatctccg cgcgcgtcat
      961 gccgatgtcg aggtgtatgg ctttcgcgcc ctcgaagagc cggtctagat gcaaccccag
     1021 aaaggcgaac tcgccgtcgt gcaggcgaag gccctcccac accccgtccc ccaccaggaa
     1081 accagagtcg aagacggata tcttcgcgtc ctcccgcctg aagaaatcgc cgtttatgta
     1141 aacgaagatg ccctcgttgc gttcgtccgg cagggcctca tgcgttcccc tggccaaccg
     1201 ttcccccctc ccactcgtcg cgcatcacgc ccatgatcac gttgtccacg taacgtccgg
     1261 caagccagac gtgctctcgc atgcggccct cctccacgaa cccgcaggcc ctgtaggccc
     1321 ggatggcccg ctcgttcgag gcgtgggtct cgagccacac gcgcctgagg ttcctgatcc
     1381 tgaacgcgta atcgaggagc aacttcacgg cctcgcgtcc gtaaccacgc ccccagtggt
     1441 ctttctcgcc gatgctgatg ccaagctcac agtggcgggc cgccacatcg aggttgaaga
     1501 ggccgcagtg cccgatggcc ctcccgtcga cctctatcat gaagtcggtc ttgtcgcggc
     1561 gggcctcacg ctcctccctc tcgaacctgg cccgctgcct ctcgaaagat aggggcagcg
     1621 gtggatcgcc acccccggct agctcgaccc ccagatcgtt ccagaatcgc cagaggacct
     1681 cctgatcctc catctccacg gcccgcagcg taactcgttc tcccctgagc atcaggcccc
     1741 cgatcctacg gggtggcgca tccacacgtt tggctccgtg tagacgccga ggtctcgctc
     1801 caggtccatc tcgaccggct gcagcgcccc ctctaccacg tagggcccgg tctcgggaaa
     1861 acgcatgccc gtccacccct cccactcgga gaccgtcccc gcgatgacca tggactccag
     1921 ggcgaccgcg acgaaccgcg cgccgagcct gtaatgtacc cgaagccacg ggtcgaaggg
     1981 cagcccgtca tccctcctcc acccggcgta tcgctccatc ggcgtgagcg ggtaccgctc
     2041 tttccacgtg ggacggaccg gtgctatcaa ggcgccaagc ccgcgtgccg ccgcgatggt
     2101 cttcatggcc ctgagcacca cgctgctgag cccacgcccc cggtgcgccg gcggtacgac
     2161 cgccagaagc gcggatacca ccgtcggggc gcctcccgac cccccgccta ctccacgctc
     2221 cagcacccca tccaaccccg ccggcaagcc atcccgcgtc ccgtcccaga aaacaggaag
     2281 gctatgcccc gcggcgagaa cctcccccgc cccatcacag accacgacct gataccccgc
     2341 aaactcggta tacagcgatg gccagtaccg ccccacaacc gcatcgtgat acataaactc
     2401 cgcaaaagcc ctcggcaaag acctcaccgc agcttccaac tccggccgtt gggccagagt
     2461 gaaaacctca atgccttcag ccaccctcac gtccacaagg tccaaatagc tgacagctga
     2521 tagctaacag ctgatagcta acagctacaa aaacgccgga accagctcgc ccttgatcag
     2581 gtcggcatca ttctctcgtt cgcgcaccac ggcccatcta tcccccctca ccatgacctc
     2641 ggctggcctg ggacgggagt tgtagttgga ggccatggag aaaccgtagg cgccggcgtc
     2701 cttcacgacg agcacctcgc cctcggctgc gtcgggcagc tcgcggtcgc gggccaggta
     2761 gtcgcctgac tcgcacacgg ggccgacgag gtcggcgttc gccacggagc cgttctcctc
     2821 gacggggaga acctcgtggt gggcgtcgta gaggctcggg cgaaggaggt cgttcatgcc
     2881 agcgtcggcc acgataaagc tcttctcgcc gctcttcttg cggtagatga ccctggtcag
     2941 gagaacgcca gcggcgcccg cgatatagcg gcccatctcg cacaggatct tcgtgcccgt
     3001 cgactccagc gtggggcgta tggcgtctac cagctccctc ggggacgggg tctcctcgtc
     3061 cttgtagggg atgccaaggc cgccgccgat gttgaagtac tggatatcga agccgcgggt
     3121 cttcagctcg tctaccaggc cggcggaacg ctccaccgac tcgctgaagg gggtgagctt
     3181 ggtgatctgg gaccctatat gctggtggac cccgataagc tccacgcacc ggtagccctc
     3241 taccctctcg gcgagcgcga gcgcctcgtc cacggggatg ccgaacttgt cgcggctcct
     3301 gcccgtggtg atgtgctcgt gggtctcggc gtccacgttc gggttgacgc gtagcgccac
     3361 gcgggcccgc ttgccgaacc tggagcagat gcggtccagg agatccagct ccgacgcgga
     3421 ctccacgttg aagaggagta tgcgctcccc gaggccggcc attagctccc gctccgtctt
     3481 cccaacgccg gcaaaaacga ccttcttggg atcgaacccg gcccgcatgg tcctgtagag
     3541 ctcgcccccc gagacgatgt cggcgccggc cccgaacgag gcgagggccc gcaacaccgc
     3601 caggttgccg tttgccttta ccgcaaagca gacgagatgg tcgagccccg agaacgcctc
     3661 gtcgagctct tcgtaagccc tctgaagtgc ggtgtggctg tagacgtagg tgggggtccc
     3721 agccccagcg gctatctcgg agagcggcac gtcctcgcag cggagtccgc ccccctcgta
     3781 atgaaagtcg tccaaaacgt acctcctcgc gtagccgggg cgtgctatat tttcggctct
     3841 gcccgacgag ccgtagggag aatatactcc gtgccgaacc cccgcgcgac accccgggcc
     3901 aatgagatca ctaccaccag ccccgcccct cccctgctcc tgaacccgat cctctcacac
     3961 cagggtgctt acccgcttct gcgcctcgac gaacgccgca aggcgctgga ggattccggc
     4021 gcggagctct tcgacttcgg caccggcgac ccgagggagc caacggacga gaggatccgc
     4081 caggccctca tagagggcgt cccggcatcg agccggtacc ccacctcatc cggcaagcgg
     4141 gagctgcgcg aggcgttctg cggctggatg gaacgccgcc acggcgtcac gctcgacccg
     4201 gagcgcgagg tcctgccggc caccggctcg aaagaggcca tcttccacgc ccccctcgcc
     4261 ttcctccacc acacccacga gcagcgcggc gtcgcctacg ggacgccagg ctaccccgtc
     4321 tacgaacgtg gaacgctgtt cgcgggcggc gaggcccggc ccgtagagct gaagggcgag
     4381 aacgggttcc tgctcccgcc cgaggccgtg gacccggggg agacccggat actctggatc
     4441 aactaccccc acaacccaac gggcgcgacg gccacctacg agtacctgga aagggttgcc
     4501 gaattctgcc gcgagcacga cctgctcctg ttctccgacg agtgctacaa cgacgtctac
     4561 tccggggagc cgccgccctc catcctggag gtgagcaggg agcgcacgct ggccttctgc
     4621 tccctctcca agcgtagcgg tatgacgggc taccgctccg ccatcatggc gggagaccca
     4681 gagatcatct cggccctcgc caagctacgc ccctccgtcg gcgtagccac ccagtctttc
     4741 gtgcaggacg ccgcgaccgc ggcctggtca gacgacgccc acgtagagaa gcggcggcgg
     4801 atcttcggcc agaaacgcgc cctgttcacc gccttcttcg accgggccgg cctcgagtac
     4861 ctgcagaccg acgcgag
//
LOCUS       DBNGVD010000545        10826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4514092, whole genome shotgun sequence.
ACCESSION   DBNGVD010000545 DBNGVD010000000
VERSION     DBNGVD010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4514092"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..898)
                     /locus_tag="AC16CM_14670"
     CDS             complement(524..898)
                     /locus_tag="AC16CM_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141123.1"
                     /translation="MDVPGLGEALAVFGFEEEVRLYIGYGDEGLRPCAVGPDDLVALL
                     LGPWSRFERITLDPMPGRGAGLMLRLASTRRGDFLDHVARKYGLGRAWRDGGPPAARR
                     GKHSLGYARPDIGLVGDSPTPE"
     gene            complement(1303..3030)
                     /locus_tag="AC16CM_14675"
     CDS             complement(1303..3030)
                     /locus_tag="AC16CM_14675"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR3141124.1"
                     /translation="MESRVPQDIERQVFDVIVIGAGINGAGIARDAAMRGLRVLLLDK
                     GDISSGTTQWATRLIHGGLRYLEHYEFALVRESLRDREALLHIAQHLVKPLGFLVPIY
                     KGNSHGPLLVRMGMNLYDTLSYDKSMDRHKMLGRKQVEEREPGLNTDGLKAAAFYYDA
                     QVEYAERVAVENAISARDHGATVVTYANVENLIIEGGNVKGVEFTDVFTGESHTARAP
                     VTVNVAGPWVDEVLAELQAPGSDDNVGRFMGGTKGSHLIVDAFPGAPEGEALYVEARK
                     DGRPYFIVPWNGRYLIGTTDLRYEENLDYVKASEEEIEYLLDETNYVIPEANLTRDDV
                     LFTYSGVRPLPYNPEGETGGVTRSHVVFDHAKGESAAGGKVKSQSAELPPVGGLLSII
                     GGKLTTYLNLSRQTTDAIFKKLDRKAPKSRARKTPLPGSETADFGSFVDGFKAASTVP
                     EVVRDRLLKLYGVRASEVLAIGEDDPDLLTPLSPIESVETALIGAEVIWAFREEMAQT
                     LSDVLLRRTMAGYGPRVARDVVEPAAQVAVQHLGWDEERAEREVNTYLEWIERYTPKD
                     FGDREPSPA"
     gene            complement(3218..4552)
                     /locus_tag="AC16CM_14680"
     CDS             complement(3218..4552)
                     /locus_tag="AC16CM_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141125.1"
                     /translation="MSSQQAQGEYGAFGNFVERIGIPRPLFWGFVAVFIFMIGDGVEI
                     SRLPGYLAGPGGLNGAQAALTTGTVYGLAVMIASWLSGTLSAIWGPQRVMQLGAAIWV
                     VFEAIFLIFAVNSGSLVLVATIYGIRGLSYPLFAFGFLVWIQTVTRVELRGSAAGWFW
                     FAFTGGLPTLGALVAAITTPLMGVYNTFWLSLVIVAIGGLIGSFAVHEAQGKQPIADE
                     HVENPRSYRRLLEGVDILWRDPRVFLGGIVRIVNTSPQYGFFYFMPFVFVVGSSASGF
                     MSGGQYATLLTLVYATNIFANLFFGIFGDRFGWRRTVTFFGCVGCAISTPLWYFASLA
                     SGSYLVCVALGMIYGALLAGFVPLSALMPSMVERHDKGAALAILNLGAGGAAFIGPAI
                     ATALNPFVGYSGVVITYSALYFICAVLTSFIRDASDPGEVGETEEEPVATTA"
     gene            complement(4625..6199)
                     /locus_tag="AC16CM_14685"
     CDS             complement(4625..6199)
                     /locus_tag="AC16CM_14685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3141126.1"
                     /translation="MSGGAKDGPYALGIDFGTESVRVGIFDMEGTPLVFTGEVYGLAH
                     PYPGWAEQDPNEWWDCLVKATRRAVDESGIAPEEIVGLSVDATTCTVVALDSQDRLLR
                     PAIMWMDVRAADQARRVAETKDAALKYNGYARVSAEWMPSKALWLKENEPDTYESAAR
                     IGECLDWITYRLTGEWTASINIASFRWYYDRDEGGFPESLYSGLGLDDLLEKLPQEVL
                     DMGKVAGELRKEVAEELGLPAGIPVAEGGGDAFVALVGLDVLEPRKMALITGSSHVIV
                     GQAAEPKHGPGLFGAYTDGIVPGQYSIEGGQASTGSVIKWFKDKFAKGAAEEGARRDV
                     DPYEVLGEWAAEVPIGSEGLMVLDYWQGNRTPHTAPEARGIMWGLSLRHGEGHVFRAI
                     MEGICYGTEDILRRMRENDFEPKEGVVCGGPTKSDLWMQMHADVSNLPISFTRVPEAP
                     TLGSAILGAVGAGVYPDIHEAARNMVHVEGGIEPDQEAHEEYKFYVDKYAETYPQMKE
                     LMHEVSHHVASRDRQQ"
     gene            complement(6196..7575)
                     /locus_tag="AC16CM_14690"
     CDS             complement(6196..7575)
                     /locus_tag="AC16CM_14690"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HIR3141127.1"
                     /translation="MSERRIDAALQEATDTRDVNIGAGALASVPDVFRENFGDGPIVV
                     VADGNTFEVAGQRVHQELEDAGYTMVEPYVFPADPPLYAEYSNIEKLRDSLQEHDAIP
                     VAVGSGTLNDIVKRSSHECDRPYMCVGTAASMDGYTAFGASIEKDGLKQTLSCPAPRA
                     VVADLEVLQNAPENMTASGYADLLGKVTSGADWLVADALEVEKIDPTGWSLVQDHLRG
                     WTDSPAELRAGYQQPTEDLVEGLIMSGLGMQSYESSRTASGAEHQFSHLWEMEGLGKD
                     DRPRLSHGFKVGVGTVAIAALYELLLQRDLSDLDIGSLQDSWPSREEMEQSVREQHTD
                     PDLEEGAVEQTLAKYVSAEELAERLELLQKVWPELREKVREQLLPAGEIKQMLEAAGC
                     PTTPEEIGLGWDEFERTYRRAQTIRKRYTALDLAFETGLLDECVEELFAPGGFWGRVA
                     ASAQTEGGR"
     gene            complement(7605..9167)
                     /locus_tag="AC16CM_14695"
     CDS             complement(7605..9167)
                     /locus_tag="AC16CM_14695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3141128.1"
                     /translation="MAEGPYLLGIDFGTGGARVGIFDPEGTPIVFTEREFTLKHPRPG
                     WAEQDPDEWWSSLVEAVRAAMDEGGVPAEEIVGISLDAMSSTVLAVDKEGRHLRPAIM
                     WMDVRSSDQADRIARTHNDALKYNGYGPVSAEWGLPKALWIKDKEPETYREASYICDC
                     GDWLVNRLTGEWAFSINFASSKYYYDRNEGGFPESLYDQVDAGDLLEKFPQDVLDLGT
                     VVGELSREAAEELGLKAGTPVAEGGVDAYVGALGLGVVEPGKMALITGSSHVMIGQTE
                     KPIHDPGFWGAYTDAMIPGQYTVEAGQASTGSIVAWFKNQFAGDAAAQARERGVDTYE
                     VLTELAHDIPIGSDGLIVLDYFQGNRSPYTDPLARGMMWGLTLSHTPGHVFRAIIEGI
                     CFGTENILRTMRGQDFEPKLNVVSGGPAKSGLWMQMHADVSNVPISFTEVSDGPALGS
                     AMLAAVGAGIYPDIPSAAEKMVHTADTIDPDPERHEEYKFYVDRYLESYPQMKELMHK
                     TERHVAGESQAE"
     gene            complement(9170..10045)
                     /locus_tag="AC16CM_14700"
     CDS             complement(9170..10045)
                     /locus_tag="AC16CM_14700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009198433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3141129.1"
                     /translation="MEQNSEAGLSGGRGVPERLYEGYVFDLDGTIYLGNELLPGAERL
                     ILKLRELDKRVIFLSNNPTKDPKMYAEKLGKLGLETPEDEIVNTVVTMTQWLVQNHPD
                     ATVFPISEEPLKRALDRAGIRMSDNPEEIDIVIASYDRGFEYSKLQIAFDAIWFHGRA
                     ILVTTNPDRYCPFPGGRGEPDAAAIVGAIEACTGAECQVNVGKPDPIMLETIMGLVGL
                     DAEECVMTGDRLYTEIRMARDAGMPSAVVLTGETTAEDLESEVEENLPDYTLERIDQL
                     IPPDLWEEFGWEEGA"
     gene            complement(10127..10591)
                     /gene="rpiB"
                     /locus_tag="AC16CM_14705"
     CDS             complement(10127..10591)
                     /gene="rpiB"
                     /locus_tag="AC16CM_14705"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR3141130.1"
                     /translation="MASIAIGADNAAFALKDVLVQHVRDEGYEVKDFGTDPDNPEDYP
                     DVAVEVAEAVARGEHERAILCCGTGLGMSITANKVPGVRAAQCHDVYSAQRARKSNNA
                     QVLTMGGRVIGPENAKTVLDAWLASEFEGGGSAPKVEKMEEIDRRYHAAREA"
BASE COUNT         1778 a         3642 c         3364 g         2042 t
ORIGIN      
        1 ttccccctca gccacaggaa cagctcggcc tcaccttccc cgctgaagac gggcagggtc
       61 ttcccgtcgt tcagggtcag tacgtccatg ggaccctctt cgttcctcgc taccagccac
      121 caggacgacc gcgagagaca gcccgaacgc ctgtatgccc gtctctgttc ggtcaacatc
      181 cgactctccc tactactagt ggccaggtcc gccagcggtt ccgtctgacc ggcgtgttgc
      241 atgcgtaagg tcccattgct tgcgacaccg ggtgcggtgc gccaacctgt agggtcgcgt
      301 accgggtccc gtctgacgtt ccgtcgcata gcgatgtgcc cccttacccg gcaccaggac
      361 gcccgtctga accggggctt gtcgcgcttt cttcgagtcc aggcacgtta tcttgtgccg
      421 ccccgtgcgt acgtatgatg ccatcagggt cccgacgggt catgcacagt tcgggccatt
      481 ctggtgtggg tggtgggggc ccggaccagc ctcgtagccc tctctactcc ggagtcggtg
      541 aatcaccgac caggccgata tcgggtcgcg catacccgag gctgtgcttg cctcttcgtg
      601 cggctggcgg gccaccgtcg cgccatgccc gcccgaggcc gtacttgcgg gcgacgtgat
      661 ccagaaagtc tccgcggcgc gtgctggcga gccggagcat gagtccggcg cccctacccg
      721 gcatcggatc gagcgtaata cgttcgaagc gcgaccaggg cccgagcaac agcgccacaa
      781 gatcatccgg tccgacggca cagggacgca aaccttcgtc cccgtagcct atatacagtc
      841 tgacctcttc ctcgaaaccg aacacagcca acgcctcgcc gaggccaggc acgtccacgg
      901 taagcggatc cgagagggta tccgggatcc gacaagcgat ccagaacctt ggataccctc
      961 cgatcgctgc ccctccttac gtccgatggc gcgctactgg tgcgatcacg ctagagaacc
     1021 ggaccctcat tattgatgtc ccgactgttc cagtcgatac atctttcggg ccaattagca
     1081 cgctgcggcc cttcgggcct cgcttgtcag cacactacgg ccttcggcct ccgtttgtca
     1141 gcaacgcttc gcctggctcc gccaggctcg ctttcagccg gatccctctg cggcctgcac
     1201 cttccgcagg acggcaacga cgccgggaag aggtggggtg ccgaagctgc cgttatggac
     1261 cgaaacgact ggtcgaaagc gaggcgaagc cagagcgtgc tctcaggccg gcgacggttc
     1321 gcggtccccg aagtctttcg gggtgtagcg ctcgatccac tccaggtatg tgtttacctc
     1381 gcgctcggcg cgttcctcgt cccacccgag gtgttggacg gcgacctgcg cggcgggttc
     1441 caccacgtcg cgggcgaccc ttggcccgta gcccgccatc gtacgcctca ggagcacgtc
     1501 ggagagcgtc tgggccatct cctcgcggaa ggcccagatc acctcggcac cgatgagcgc
     1561 cgtctcgacg ctttcgatgg gagagagcgg cgtgaggagg tcggggtcgt cttcgccgat
     1621 ggccagcacc tcggacgccc tgaccccgta gagcttgagc agcctgtcgc ggaccacctc
     1681 gggaaccgtg ctcgccgcct tgaacccatc cacgaaggac ccgaagtccg cggtctcgct
     1741 ccccggcagg ggcgtcttgc gggccctgct cttgggcgcc ttcctgtcaa gcttcttgaa
     1801 gatggcgtcc gtggtctggc ggctcaggtt caggtaggtg gtcagcttac cgccgatgat
     1861 agaaaggagt cccccgaccg gtggcagctc ggcgctctgg ctcttcacct tgccgccagc
     1921 cgccgactcc cccttggcgt ggtcgaagac cacgtggcta cgcgtaacgc cgcccgtctc
     1981 tccctccggg ttgtagggca ggggccttac ccccgagtag gtaaacagca cgtcatcgcg
     2041 agtcaggttg gcctcgggga tcacgtagtt cgtctcgtcg aggaggtatt cgatctcctc
     2101 ctcgctcgcc ttgacgtagt ctaggttctc ctcgtagcgg aggtcggtgg tgcctatcag
     2161 atacctaccg ttccacggca cgatgaagta aggcctgcca tccttgcggg cctcgacgta
     2221 gagcgcctcg ccctcagggg cgccggggaa ggcgtcaacg atcaggtggc tccccttggt
     2281 gccacccatg aagcgcccca cgttatcgtc ggagccgggc gcctggagct cggcgagcac
     2341 ttcatccacc caggggcctg ccacgttgac cgtgacgggg gcccgggcgg tatggctctc
     2401 gccggtaaag acgtcggtga actccacgcc cttgacgttg ccgccctcga tgatcaggtt
     2461 ctccacgttg gcgtaggtga cgacggtcgc accgtggtcg cgggcggaga tggcgttctc
     2521 cacggcaacc cgctcggcgt actcgacctg cgcgtcgtag tagaaggcgg cggccttcag
     2581 gccgtccgtg ttgaggccgg gctcgcgctc ctcgacctgc ttgcggccga gcatcttgtg
     2641 acggtccatg ctcttgtcgt aggagagcgt gtcgtagagg ttcataccca tacggaccag
     2701 gagcggcccg tgggagttgc ccttgtagat ggggaccagg aagccaagtg gtttcaccag
     2761 gtgctgcgcg atgtgcagca gcgcctccct gtccctcagg gactccctga ccagggcgaa
     2821 ctcgtagtgt tccaggtaac gtagcccgcc gtggatcagg cgcgtggccc actgcgtggt
     2881 cccggaggag atgtcgcctt tgtccagaag cagtaccctg aggccccgca tcgccgcgtc
     2941 gcgggcgatg ccagcgccgt tgatgccggc cccgatcacg ataacgtcga agacctgccg
     3001 ctcgatatcc tgcggtacgc gtgactccat ctctatcctt cccgcgctat gtgtatctga
     3061 cgctttctct aatgctacaa cggaacaggg gcggcggatg ggtggaaagt cacctcatca
     3121 gccgtcagcc gtcagctatc agccaaaaag ctggccgctg atagctaacg gctgacagcg
     3181 cggttcgcaa aaggattgcc tttttgcgaa ccgttctcta ggccgtcgtg gcgacgggct
     3241 cttcctccgt ctctcctacc tcgcccggat ccgacgcgtc cctgataaac gacgtcagca
     3301 cggcgcagat aaagtacagg gcagagtagg tgatgaccac gcccgagtag ccgacgaagg
     3361 ggttgagggc ggtggcgatg gcaggcccga taaacgcggc accgccggcg ccgaggttga
     3421 ggatggcgag cgctgcgccc ttgtcgtggc gttcgaccat ggagggcata agcgccgaga
     3481 gcggcacgaa accggcaagc aacgcgccgt agatcatgcc gagcgccacg cagaccaggt
     3541 agctgccgga agccagcgag gcgaagtacc atagcggggt cgagatggcg cagcccacgc
     3601 acccgaagaa ggtaaccgtg cgccgccacc cgaagcggtc accgaatatg ccgaagaaca
     3661 ggttggcgaa gatgttggtc gcgtacacca gggtcagcag ggtggcgtac tgccccccgc
     3721 tcatgaagcc gctggcgctg ctcccgacca cgaagacgaa cggcatgaag tagaagaacc
     3781 cgtactgggg cgaggtgttg acgatgcgca cgatgccacc aaggaacacc ctggggtcgc
     3841 gccacaggat gtcgacgccc tccaggagcc tgcggtagct cctcgggttc tcgacgtgct
     3901 cgtcggctat gggttgcttg ccctgggcct catgaacggc gaaggagcca atgaggccac
     3961 cgatggccac aattacgagc gagagccaga aggtgttgta cacgcccatg aggggggtag
     4021 ttatggcggc cacgagggcg ccgagcgttg gcaggccgcc ggtaaaggcg aaccagaacc
     4081 atccagccgc ggatcctcta agctccacgc gggtgacggt ctggatccag accagaaaac
     4141 cgaaagcgaa gagcgggtag gacaggcccc tgatgccgta gatggtcgcc acgagcacca
     4201 ggcttcccga gttgacggcg aagatgagga agatggcctc gaacacgacc cagatagcgg
     4261 cgcccagctg catgacccgc tgcgggcccc agatggccga gagcgtgcca gagagccacg
     4321 aggcgatcat cacggcgagg ccgtagaccg tgcccgtcgt cagggcggcc tgcgcgccgt
     4381 tcagtcctcc gggtcccgcc aggtatccgg ggagacggct gatctcgacg ccgtccccga
     4441 tcatgaatat gaagacggcg acgaagcccc agaagagggg cctggggatg ccgatgcgct
     4501 cgacgaagtt accgaaggcc ccgtactcac cctgtgcctg ttgactgctc accctatcac
     4561 tcctcctttt tctgtgtgtc cacgcggccc gaagccgcac gccttgcgtt cgcccgtggc
     4621 ggccttactg ttgcctgtcc cggctggcca cgtggtggga aacctcgtgc ataagctcct
     4681 tcatctgggg ataggtctcg gcgtacttgt cgacgtagaa cttgtactcc tcgtgcgcct
     4741 cctggtctgg ttcgatacca ccctcgacgt ggaccatgtt gcgggcggcc tcgtggatgt
     4801 cggggtagac cccggcccca acggcaccaa ggatcgcgga gccgagggtg ggcgcctctg
     4861 gcaccctggt gaacgagatc ggcaggttcg agacatcggc gtgcatctgc atccacagat
     4921 cgctcttggt tggtccgccg cagacgacgc cctcttttgg ctcgaagtcg ttctcgcgca
     4981 tcctgcgcag gatgtcctcg gtgccgtagc agataccctc catgatggcc ctgaagacgt
     5041 ggccctcacc gtgccgcagg gagaggcccc acatgatgcc gcgggcctcg ggggccgtgt
     5101 gcggcgtgcg gttgccctgc cagtaatcga gcaccatgag cccctcggag ccgatgggca
     5161 cctcggcggc ccactcgccg agcacctcgt aggggtccac gtcgcggcgg gccccctctt
     5221 cggcggcgcc cttggcgaac ttgtccttga accacttgat cacggagccc gtggaggcct
     5281 ggccgccctc gatcgagtac tgccccggca cgatgccgtc ggtgtaggca ccgaagagcc
     5341 cgggcccatg ctttggctcg gccgcctggc caacgatcac gtgtgaagag cccgtgatga
     5401 gggccatctt gcggggctcg agcacgtcga ggccgacgag ggcaacgaag gcgtcgccac
     5461 caccctccgc gacgggtatc ccggcgggta gcccaagttc ctcggccacc tccttgcgta
     5521 gctcaccggc gaccttgccc atgtcgagga cctcctgggg gagtttctcc agaaggtcgt
     5581 cgagccccag acccgaatag aggctctcgg ggaagccgcc ctcgtccctg tcgtaatacc
     5641 agcggaagct tgcgatgttg atggaggcgg tccactcgcc ggtgaggcgg taggtgatcc
     5701 agtccaggca ctcaccgatg cgtgcggccg actcgtaggt gtccggctcg ttctccttga
     5761 gccagagcgc cttggagggc atccactcag ccgataccct ggcgtacccg ttgtacttga
     5821 gcgcggcgtc cttggtctcc gcgacccgcc gagcctggtc tgcggccctg acgtccatcc
     5881 acatgatggc cggccgcagg agcctgtcct gactatcgag ggcgaccacg gtgcaggtcg
     5941 tggcatccac ggagaggccg acgatctcct ccggcgcgat cccgctctca tctaccgccc
     6001 gccgggtcgc cttgaccagg caatcccacc actcgttggg gtcctgctcg gcccagcccg
     6061 gataggggtg ggcgaggccg tagacctccc cggtaaagac gagtggggtc ccctccatat
     6121 cgaagatgcc gacccttacg ctctccgtcc cgaagtctat accgagggcg taaggtccat
     6181 ctttcgctcc cccgctcatc gccctccttc cgtctgcgcg gaggccgcga cccggcccca
     6241 gaagcccccg ggtgcgaaca actcttccac gcactcgtcg agcaggccgg tctcgaaggc
     6301 gaggtcgagg gcggtatagc gctttcggat agtctgcgcc ctcctgtaag tcctctcgaa
     6361 ttcgtcccag ccgagcccga tctcctcggg cgtagtgggg caaccggcgg cctcgagcat
     6421 ctgctttatc tcaccggccg gcagcaactg ctccctgacc ttctcgcgca gctcgggcca
     6481 tactttctgg agcagctcca gcctctcggc gagctcctcc gcgctgacgt acttggctag
     6541 cgtctgctcg acggcgccct cttcgaggtc ggggtcggtg tgttgctccc gaacggactg
     6601 ctccatctct tccctggagg gccaggagtc ctgcagagac ccgatgtcca ggtcggacag
     6661 gtcgcgctgg agcaagagct cgtagagggc cgctatggcg accgttccaa cccccacctt
     6721 gaaaccgtgg gagaggcgcg ggcggtcgtc cttgcccaac ccttccatct cccagaggtg
     6781 gctgaactgg tgttcggcgc ccgaggcggt cctcgaagac tcgtaagact gcatcccgag
     6841 gccagacatg atcagaccct ccaccagatc ctcggtgggc tgctggtacc ccgcgcgcag
     6901 ctcggccggg ctgtcggtcc acccgcgcag gtggtcctgc accagcgacc agcccgtcgg
     6961 gtcgatcttc tcgacttcga gggcgtctgc gaccagccag tcggcccccg aggtcacctt
     7021 gcccaacagg tcggcgtacc ctgaggccgt catgttctcg ggggcgttct gaaggacctc
     7081 gaggtcggcc accacggccc gcggggccgg gcaggagagc gtctgcttca acccgtcttt
     7141 ctcgatggaa gccccgaagg ctgtataccc gtccatggag gcggcggtgc ccacgcacat
     7201 atagggtctg tcacactcgt gcgaggaacg cttgacgatg tcgttgagcg tccctgagcc
     7261 cacggccacg gggatggcat cgtgctcttg cagggagtcc ctgagcttct cgatgttgga
     7321 gtactcggcg tagagcggtg gatctgccgg aaacacgtag ggctccacca tggtgtaccc
     7381 ggcgtcttcg agctcctggt ggacccgctg gcccgccacc tcgaaggtgt tgccgtcagc
     7441 gaccactact atggggccgt ctccgaagtt ctccctgaat acgtcgggga cggaggcgag
     7501 tgcgcccgcc ccgatgttca cgtccctcgt gtcggtggcc tcctgtaggg ccgcgtctat
     7561 gcgcctctcg ctcataagtt ccttccagtc atgtcaggtg cgtcctactc cgcctgagat
     7621 tcgcccgcga cgtggcgctc cgtcttgtgc atcagctcct tcatctgggg gtaggactcc
     7681 aggtagcggt ccacgtaaaa cttgtactcc tcgtggcgct ctgggtccgg gtctatggtg
     7741 tcggcggtgt ggaccatctt ctcggccgcg gacgggatgt ccgggtagat gccagccccg
     7801 acggcggcca gcatggccga gccgagggcc gggccgtcgc taacctccgt gaacgagatg
     7861 ggcacgttcg agacgtcggc gtgcatctgc atccacaggc cgctcttggc cgggccaccg
     7921 gagaccacgt tgagctttgg ttcgaagtcc tgcccgcgca tggtgcgcag gatgttctcc
     7981 gtgccaaagc aaataccctc gatgatggcc ctgaagacgt gccccggcgt gtggctcagc
     8041 gtcaggcccc acatcatgcc ccgggccagg ggatccgtgt aaggcgatct attgccctgg
     8101 aagtagtcga gcacgatgag cccgtctgag ccgatcggta tgtcgtgcgc aagctcggtc
     8161 aggacttcgt aggtatcgac gccgcgctct ctggcctggg ccgccgcgtc gccggcgaac
     8221 tggttcttga accaggccac gatggatccg gtcgaggcct gacccgcctc cacggtgtac
     8281 tggccgggga tcatggcgtc cgtgtaggct ccccagaagc cggggtcgtg tatgggcttc
     8341 tctgtctgac cgatcatgac gtgcgaggag cccgtgatga gggccatctt ccccggctcg
     8401 accaccccga ggccgagcgc cccgacgtag gcgtcaaccc caccctcggc caccggcgtg
     8461 ccagccttga gcccgagctc ctcggcggcc tcccggctca actcaccgac caccgtaccg
     8521 aggtcgagca cgtcctgggg aaacttctcg agcagatccc ccgcgtctac ctggtcgtag
     8581 aggctctcgg ggaagccgcc ctcgtttcgg tcgtagtagt acttggagct tgcgaagttt
     8641 atggagaaag cccactcgcc ggtgaggcgg ttgaccagcc agtcgccgca atcgcagatg
     8701 tagcttgctt ccctgtaggt ctcgggctct ttgtccttga tccagagcgc cttcgggagc
     8761 ccccactcgg ccgagacagg accgtagccg ttgtacttga gggcgtcgtt gtgggtgcgg
     8821 gcgatcctgt cggcctggtc ggaggagcgt acgtccatcc acatgatggc cggccgcagg
     8881 tgccgcccct ctttgtcgac cgcgagaacg gtgctgctca tggcgtcgag cgagatgccc
     8941 acgatctctt cggcgggcac gccaccctcg tccatcgctg cccttaccgc ctcgacgagc
     9001 gatgaccacc actcgtcggg gtcctgctcg gcccacccgg gccgcgggtg cttcagcgtg
     9061 aactcccgct cggtaaagac gatcggcgtc ccttctggat cgaagatgcc gaccctggcc
     9121 ccacccgtcc cgaagtcgat tccaaggaga tacggacctt cggccatggc tatgctcctt
     9181 cctcccagcc aaactcctcc cagagatcgg gcgggatgag ctggtctatt ctttcgaggg
     9241 tgtagtctgg taagttctcc tcgacctccg actccagatc ttcggctgtg gtctccccgg
     9301 tcaggacgac ggcggagggc atgcccgcgt ctctggccat gcggatctcc gtgtagagcc
     9361 ggtcgccggt catgacgcac tcttcggcat cgagcccgac caggcccatg atggtctcga
     9421 gcattatggg gtcgggcttg cccacgttga cctggcactc ggcccccgtg cacgcctcga
     9481 tggcccctac gatggccgcc gcgtccggct ctcccctgcc ccccggaaag gggcagtacc
     9541 ggtcggggtt ggtggtgacg agaatcgccc gcccgtgaaa ccagatggcg tcgaaggcta
     9601 tctggagctt gctgtactcg aacccgcgat cgtagctggc gatcacgatg tctatctcct
     9661 cggggttatc ggacatcctg atcccggctc tatcgagcgc cctcttgagc ggctcctcgc
     9721 ttatggggaa gaccgtggcg tcgggatggt tctgcaccag ccactgcgtc atggtgacga
     9781 cagtgtttac tatttcatcc tctggtgtct ccaaccccag cttgccgagc ttctcggcgt
     9841 acatcttcgg gtccttggtc gggttgttgg agaggaagat gacgcgcttg tcgagctcgc
     9901 gcagcttgag gatcagacgc tcggccccgg ggagcaactc attccccagg tagatggtcc
     9961 cgtcgagatc gaagacgtac ccctcgtaca gccgctccgg caccccccgc ccaccggaaa
    10021 gcccggcctc gctattttgc tccatgcggt ctctcaaccc cctgccaccg gtagataccc
    10081 ccaggtgcca gcccgaaacg gtggcgtctc gtcggcagtg ccgtccctag gcctccctcg
    10141 cggcgtggta gcggcggtcg atctcctcca tcttctccac cttgggcgcc gagcctcccc
    10201 cctcgaactc ggaagcgagc caggcatcaa gcacggtctt ggcgttctcc ggcccgatca
    10261 ccctcccgcc catggtcaag acctgcgcgt tgttcgactt gcgagccctc tgcgcagaat
    10321 acacgtcgtg gcactgcgcc gcccttaccc caggcacctt gttggccgtg atggacatcc
    10381 caagcccggt cccgcaacat agtatggccc gctcgtgctc acccctggcc acggcctcgg
    10441 caacctcgac cgccacgtca gggtagtcct ccggattatc agggtccgtg ccgaagtcct
    10501 ttacctcgta gccctcgtcg cgcacatgct gcaccagcac atccttgagc gcaaacgccg
    10561 cgttgtcggc ccctatcgct atgctcgcca tctccccctt gcctccttac cgatatctgt
    10621 ccgtagaccg aatatagaag gggcgtctta cccctccaca cagatcatcg gacacactga
    10681 gcagtacact gagcagtaca tcccacaggt gcccaacgcc tagcgtctgt cgtgtccctt
    10741 tcccccatgt ggcgccagcg tagcggatcg aagatggcgt gtcaaacgtg catgttatgc
    10801 acttatgtgc agcgaaactc ttgtcg
//
LOCUS       DBNGVD010000546         6723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3786564, whole genome shotgun sequence.
ACCESSION   DBNGVD010000546 DBNGVD010000000
VERSION     DBNGVD010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3786564"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="AC16CM_14710"
     CDS             complement(<1..416)
                     /locus_tag="AC16CM_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141131.1"
                     /translation="MRVLHLRLIFNALAMAVCAVGAAMVVPAVYSLLRTPREAWVFWF
                     PGVVALAAGAILYYLTRRPPRSYVSRQSIFFMVVACWLVVVLVGTAPFMLSGVMGPVD
                     SFFNSMAGFTTTGAATVNPEQLPSALLLWRSLSQWIG"
     gene            638..1333
                     /locus_tag="AC16CM_14715"
     CDS             638..1333
                     /locus_tag="AC16CM_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR3141132.1"
                     /translation="MIGTGTAINIVAVLAGGTLGTVVGSRLSEGMRETAMNAIGLVTV
                     LVGIQSFLRFDNPLVPLVSVIVGLVIGELLGIDDVLKRFGDALERRFSKGGSPVSRAF
                     VTTSLLFCVGPLTILGSLQDGISGDYRLLALKSALDFVASLSFASVLGWGVLLSAGSI
                     LVVQGGLTLSGALFGSFMTEPMILALTSTGGVLLLGLGLTLLSLKEVRVANMLPALVV
                     APVLVAAAPLWPL"
     gene            1409..1633
                     /locus_tag="AC16CM_14720"
     CDS             1409..1633
                     /locus_tag="AC16CM_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141133.1"
                     /translation="MLDENDRHWRGSPLDMSGNTIDAYGVKEVVIEFDNGDQDEFRPR
                     TREEFGSYELHMMSAYLDSIAHRLRKGHKN"
     gene            1662..1886
                     /locus_tag="AC16CM_14725"
     CDS             1662..1886
                     /locus_tag="AC16CM_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141134.1"
                     /translation="MRGIVRTRKENSPLEGGVGLTRIVFRYSDGRELIFVPDAGREAF
                     SEDDILELKNVVDRTSAHAEWSDINTRLGF"
     gene            complement(1905..2147)
                     /locus_tag="AC16CM_14730"
     CDS             complement(1905..2147)
                     /locus_tag="AC16CM_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141135.1"
                     /translation="MAQSVLGKIEELSEERERLLEREGAHRAGNAVHGRLERIDHDLQ
                     VLWDLRRRELAGEIIDLDDDFFDRFTVDPGTDAPGR"
     gene            2241..2648
                     /gene="mce"
                     /locus_tag="AC16CM_14735"
     CDS             2241..2648
                     /gene="mce"
                     /locus_tag="AC16CM_14735"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3141136.1"
                     /translation="MLNRIYHLGYAVEDIEAAAAFYRENFGATPGEPEVVEEQCIVAT
                     MFRVGESSIELVQPTRPDSPVGKFLSKRGEGFHHVAFEVDDLRAALAELKRNGVELID
                     EEPRRGAGGMKIAFVHPGGAFGVLTELVELPHA"
     gene            2669..4267
                     /locus_tag="AC16CM_14740"
     CDS             2669..4267
                     /locus_tag="AC16CM_14740"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3141137.1"
                     /translation="MHTERRRDERRTESGIEVKQFYKQDDLEPFDYSGKLGDPGEYPY
                     TRGPYPTMYRGRPWTMRQYAGFGTARETNARFKYLTENGQTGLSVAFDLPTQMGRDSD
                     HRLSLGEVGKVGVAIDSLLDMRELFDGIPLREVSTSMTINATASILLLLYSLVAEEQG
                     VEQENVTGTTQNDILKEYLARGTYIYPPAPSMRITTDTFAYCAAELPRWNTISISGYH
                     IREVGSTAVQELAFTLANAIAYVEAALSAGLEVDEFAPRLSFFFNAHNDLFQEVAKYR
                     AARRMWSSIMRDRFGATDERSLKLRFHTQTAGSSLTAQQAENNVVRTTVQAISAVLGG
                     TQSLHTNAFDEALALPTERSARLALRTQQILASEGGLTDTADPLAGSYFVEAMTDAVE
                     QEAWEYIRRIDELGGSVKAVEERFMQQEIEDSAYRYQREVEREERIIVGVNRYVTEGS
                     DDPDMELHTVDETIRDRQTERLQELKETRDSAAVERALADLKVAAEGSANLLYPMKEA
                     LSELATLGEVSNTLRGEFGEYRPV"
     gene            4421..5635
                     /locus_tag="AC16CM_14745"
     CDS             4421..5635
                     /locus_tag="AC16CM_14745"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3141138.1"
                     /translation="MADVVDWFRRRSGRNEVPNSAEDLGVETETAEDLVDRTPREEEP
                     ADGFSEAGIREALRDVRDPEIGRDLVSLNMIRSIKIEGPGVTVGVALTTAGCPMKHRI
                     ITDIKDRLQMIEGVGSVEVDFGVMTDDDRQNLLTSLHGGPSELAPAFRDESKTRIIAV
                     VSGKGGVGKSTVAVNLAAALDRAGHSVEILDADVHGSSIPVMLGSLGKPNVVDGVIFP
                     IESDQGLKFISMGNFVNEGQAIIWRAPIVNKALTQLMRDVYWDEPDFIIVDMPPGTGD
                     VALTVAQMIPKAEALVVTTPQADAARVAVKAGKMAVQAHLKVAGVVENMGYAECPDCG
                     KELRIFGGDGGDRVAGELGSEVMGRIPILPDASGEPGRSLFDPDSAPAHAFDEIAASL
                     AATRVRRRIKVL"
     gene            5965..6291
                     /locus_tag="AC16CM_14750"
     CDS             5965..6291
                     /locus_tag="AC16CM_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141139.1"
                     /translation="MQTGGAIQGANTIPPQGRLNGLLALVAVLAIAGFMALVMWAGPL
                     GSGGVETRETVPAAGAGTAIVHDDAGQVNRGLAPGYAPVHDDAGNVNAGTGTAIVHDD
                     AGNVNR"
     gene            complement(6386..6673)
                     /locus_tag="AC16CM_14755"
     CDS             complement(6386..6673)
                     /locus_tag="AC16CM_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141140.1"
                     /translation="MGRSRNTGTEHTGTEHTGTERIRIFIGIATALWLLAAVAAVVDR
                     DALMAVSWSCLAVFGGLVSSGAAHRSHGLGYLAIALVVVGVAISIGVFMVD"
BASE COUNT         1303 a         2086 c         2153 g         1181 t
ORIGIN      
        1 ccgatccact ggctcaggct gcgccagagc agcagggctg acggcaattg ttccgggttg
       61 acagtcgcgg cccccgtggt cgtgaagccg gccatggagt tgaagaatga gtctaccggg
      121 cccattaccc ccgagagcat gaacggcgcg gtaccaacca gcacgaccac cagccagcag
      181 gcgactacca tgaagaatat gctctgcctg gaaacgtagc tgcgcggtgg cctgcgcgtg
      241 agatagtaga ggatcgcgcc ggcggccagg gcgacgacac cgggaaacca gaagacccag
      301 gcctcacggg gggttctaag cagcgagtag accgcaggga cgaccatggc cgcgccgacc
      361 gcgcagacgg ccatcgccag cgcgttgaag atcaaccgga ggtgcaacac cctcaaggag
      421 ttctacctcc ggtcgtcggg atgttcatct gcatgccgac cacgccttct tttatccttc
      481 ccggatcctg gtccaagaac ccactcatgt tcatgatata taaaccgaag gccggccggg
      541 tgcctggcat cggagataga cttcctgatc gccagcgtgc attcctttct cgaggcgcac
      601 actgtagcct ctttcctcgt gccctacact agggcgcgtg atcggaacgg gcacagcgat
      661 caacatcgtg gcggtactgg caggggggac cctgggcacg gtggtcggta gccggctctc
      721 cgaggggatg cgtgagacgg cgatgaacgc catcgggctt gtcacggtcc ttgttgggat
      781 ccagagtttc ctgcgcttcg acaacccgct cgttccgctg gtcagcgtga tcgtgggcct
      841 cgtcatcggc gagctcctcg gcatagacga tgtcctcaaa cgctttggtg acgcgctgga
      901 gcggcgcttc tcgaagggcg ggagccctgt tagccgggcc ttcgtcacca ccagcctgct
      961 gttctgtgta gggcccctga ccatactggg atccctgcaa gatgggatct ccggcgatta
     1021 ccggctgctc gccttgaaga gcgccctcga cttcgtggcc tcgctctcct tcgcctccgt
     1081 gctcgggtgg ggcgtgcttc tctccgccgg atccatactg gtcgtgcagg gcggcctgac
     1141 gttgtcaggg gctcttttcg gctctttcat gacggagccc atgattctgg ccctcacctc
     1201 caccggcggc gtgttgcttc tggggctggg gcttacgctc ctgagcctga aggaggtacg
     1261 cgtcgcgaac atgctaccgg cgctcgtggt ggcaccggtg cttgtcgcag cggccccgct
     1321 gtggccgctc tgactgacgc gcccgccacg aataatctaa tatgcacacg tagtggatac
     1381 agggtcaggg acgctacgga gaggagagat gttggacgag aacgacaggc actggagggg
     1441 cagcccgctc gacatgtcgg ggaacacgat agacgcttac ggtgtgaaag aggtcgtcat
     1501 agagttcgac aacggtgacc aggatgaatt tcgtccaagg acgcgcgagg agttcggctc
     1561 ctacgagcta cacatgatgt cggcctacct cgactccatc gcccacaggc tccgcaaggg
     1621 ccacaagaac tagtaacggg aatacccgaa aggagccagg catgcggggc atagtgcgaa
     1681 cgcgtaaaga gaattcaccc ctggagggcg gcgtcgggct tacccggatc gtgttccgct
     1741 actccgacgg gcgcgaactg atcttcgtgc ccgacgcggg tcgtgaagcg ttctcggagg
     1801 atgacatact cgagctgaag aacgtggtag accgcacctc cgcccacgca gagtggtcgg
     1861 atatcaacac gcggctgggc ttctagggct gttcggaacc ccggctagcg gccaggggcg
     1921 tcggtacccg ggtcgaccgt gaagcggtcg aagaagtcgt cgtcgaggtc tatgatctcc
     1981 ccagccagct ccctgcgcct gaggtcccac aatacttgca ggtcgtggtc tattctctcg
     2041 agccgaccgt ggacggcgtt cccggcgcgg tgcgccccct ccctttccag gagccgctcg
     2101 cgctcctcgg agagctcttc gatctttccc aacacgctct gggccatgtc cgcctcccgt
     2161 cctatgcttt ccttcgattc tagcccatct cgatacacta gaatcacggt ttgcgcgaat
     2221 gtttacggtc agggaggggc atgctgaaca ggatctacca tcttggctac gccgtcgagg
     2281 acatcgaggc cgccgctgcg ttctaccggg aaaacttcgg ggccacgccg ggagagcctg
     2341 aggtggtaga ggaacagtgc atcgtcgcga ccatgttccg ggttggtgaa tcgtcgatcg
     2401 agctcgtcca gcccacccgc cccgactctc cggttggcaa gttcctgagc aaacgtgggg
     2461 agggcttcca tcacgtagcc ttcgaggtcg atgacctgag ggccgccctc gcggagctga
     2521 agcggaacgg tgtcgagctg atagacgagg agccaaggag gggagcgggg ggaatgaaga
     2581 tcgccttcgt tcaccccggc ggggccttcg gcgttctgac ggagctagta gaattgccgc
     2641 acgcctgagt caagcggaag gaaggcccat gcacaccgag agacgccggg acgagcgccg
     2701 aacggaatcc ggtatagagg tcaagcagtt ctacaaacaa gacgacctgg agcctttcga
     2761 ctactccggc aagctcgggg accctggcga atacccctac acccgtggtc cctatccgac
     2821 catgtacagg ggcagaccct ggaccatgcg ccagtatgcc ggtttcggca cggccaggga
     2881 gaccaacgcc cgcttcaagt acctgacaga gaacgggcag accgggctct cggtggcttt
     2941 cgaccttccc acgcagatgg gccgcgactc cgaccaccgg ctctcccttg gagaggtcgg
     3001 aaaggtgggt gtggccatcg actccctcct cgacatgcgc gagctgttcg acggcattcc
     3061 tttgcgcgag gtctcgacct ccatgaccat aaacgctacc gcctccatcc tcctgctcct
     3121 ctactcgctc gtcgccgagg aacagggtgt ggagcaggag aacgtgacgg gcaccacgca
     3181 gaacgacatc ctgaaggaat acctggccag gggaacctac atctacccgc cggcaccctc
     3241 catgaggatc accaccgaca cgttcgccta ctgcgccgcc gagctgccac gctggaacac
     3301 gatctccata agcggctatc acatcaggga ggtcggctcg accgccgtgc aggaactcgc
     3361 gtttaccctc gcaaacgcca tagcctacgt ggaggcggcg ctctcggcgg ggctggaggt
     3421 ggacgagttc gccccgaggc tctcgttctt cttcaacgca cacaacgacc ttttccagga
     3481 ggtggccaag tacagggctg cgaggaggat gtggtcctcc ataatgcgcg accgtttcgg
     3541 cgcaacagac gagcgtagct tgaagctccg cttccacacg cagacggccg gctcctccct
     3601 gacagcgcag caggccgaga acaacgtggt caggacgacc gtccaggcca tctccgcggt
     3661 cctcggcggc acccagagtt tgcacaccaa cgcctttgac gaagccctcg cgctcccaac
     3721 cgagcggagc gcccgcctcg cgctcaggac gcagcagatc ctcgcctccg aaggtggcct
     3781 gaccgatacc gccgatcccc tggccggctc ctacttcgtc gaggccatga ccgacgcggt
     3841 cgaacaggag gcgtgggagt acatccggcg gattgacgag ctgggtggtt ccgtgaaggc
     3901 cgtcgaggag cgcttcatgc agcaggagat cgaggactcc gcctaccgct accagcggga
     3961 agtggagagg gaagagcgga tcatcgtcgg cgtgaaccgc tacgtgaccg aaggctccga
     4021 cgatcccgac atggagctcc acaccgtcga cgagaccatc cgcgaccgtc agaccgaacg
     4081 gcttcaggag ctgaaagaga ccagggacag cgcggccgta gagcgggccc tggccgatct
     4141 gaaagtcgcc gcggaaggat cggccaatct cctttacccg atgaaggagg ccctgtccga
     4201 gctcgcgacg ctgggcgagg tctcgaacac tttgcgcggt gagttcggcg agtaccggcc
     4261 cgtctgaagg catatgatcg caggctaaag gcatcggggc tttgcggttg cataaggtct
     4321 gattaggccg tttgaccctg tgttaacatc tgccttaagc acggagtttt tccgctcttt
     4381 tcgggctagt tgtgatagcc ggccgtagtg ccggaggagg atggcagacg tggttgactg
     4441 gtttcgcagg cgctccggta ggaacgaggt gccgaacagc gcggaggacc taggcgtcga
     4501 gaccgagacc gccgaggacc tggtggaccg cacgccgcgg gaggaggaac ccgccgacgg
     4561 gttctcggag gccggtattc gcgaggcgct cagggatgtg agggacccgg agatcgggcg
     4621 cgacctcgtg tcgctcaaca tgatccggag catcaagata gagggcccgg gggttactgt
     4681 cggcgtcgcc ctcaccactg cgggctgccc gatgaagcac aggatcatca cggatatcaa
     4741 ggaccgtctg caaatgatcg agggcgtcgg aagcgtcgag gtcgacttcg gcgtcatgac
     4801 cgacgacgat cgccagaacc tgctcacctc cctgcacggt ggcccctccg agctggcccc
     4861 agccttccgg gacgagtcga agacccgcat catcgcggtc gtgagcggca agggcggcgt
     4921 gggcaagagc accgtcgccg tgaacctggc cgccgcgctc gaccgggccg ggcactctgt
     4981 ggagatcctc gacgccgacg tgcacggctc ctctattccc gtgatgctcg ggtcgctggg
     5041 caaaccaaac gtggtcgacg gcgtcatttt cccaatcgag tccgaccagg ggctgaagtt
     5101 tatctccatg ggcaacttcg tgaacgaggg ccaggctatc atctggcgcg ctccgatcgt
     5161 gaacaaggca cttacccagc tgatgcgcga cgtgtactgg gacgagccag acttcataat
     5221 cgtggatatg ccgccaggca cgggcgacgt ggccctgacg gtggcgcaga tgatccccaa
     5281 ggccgaggcg ctcgtggtaa cgaccccgca ggccgacgct gcgcgcgtgg cggtcaaggc
     5341 cggcaagatg gccgtgcagg cccacctcaa ggtggctggc gtggtcgaga acatgggcta
     5401 cgccgagtgc ccagactgtg gcaaggagct caggatcttc gggggtgacg gtggcgaccg
     5461 ggtggcaggg gagctcggct cggaggtcat gggccgcatc ccgatcctgc ccgacgcctc
     5521 tggggaacca ggccgctccc tcttcgaccc cgactcggcc cccgcccacg ctttcgacga
     5581 gatcgctgcc tccctggccg cgactagggt gcgccgccgg atcaaggtgt tgtagcctcc
     5641 agtccgcaag tttcaggtaa gtcaagaacg ggccccgtga accgcacggg gccatttctc
     5701 tgctcctcgc agctctagat tcgccttgcg tcaggccgga tgaggccgct ccaccgcaac
     5761 ttcagaaaaa cgttacgaaa ttttagaaat cactttttcg taacgttttt cgtaacggct
     5821 gggagacggt cggccggtac attggtgtca gatgtaaagg cgggagaaag gagaacgggg
     5881 gtgatgagga caggcaccct tgtcggacgg ccgggaagaa gggatcccgg tgtccacgcg
     5941 gaccattggg aaggaggagc agagatgcaa acaggtggcg cgatacaagg agcgaacacg
     6001 atacccccac agggcaggct caacgggctg ctggccctcg tggccgtgct ggcgatagcc
     6061 gggttcatgg cgctggtgat gtgggctggc ccccttggct ccggcggggt ggagacgaga
     6121 gaaacggtcc ccgccgcagg agcgggtact gcgatcgttc acgacgacgc tggacaggtg
     6181 aacaggggtc tggctcccgg gtatgccccg gtccacgacg acgctggcaa cgtgaacgcc
     6241 ggaacgggga cggccatcgt tcacgacgac gcgggcaacg tgaaccgcta gcagggacag
     6301 acaaggagcg gagaggtccg gggtcagagt gtgcccccgg acctctccgc gttgatgtat
     6361 tcgcgaacgg gcttctagga cactactaat cgaccatgaa gacgcctatg gagatggcca
     6421 cgccgaccac caccagggca attgcaaggt agcctaggcc atgcgacctg tgggctgccc
     6481 cactggacac cagccccccg aacaccgcca gacaagacca ggatacggcc ataagcgcgt
     6541 cgcgatcgac cacggcggcc accgccgcca ggagccacaa cgccgtggcg atcccgatga
     6601 atattcggat tcgctctgta cctgtatgct ctgtacctgt atgctctgta cctgtattcc
     6661 tggacctacc catcttctcc tcccggtcgt gttcgcaggt gcaacagtta ccattctctc
     6721 atc
//
LOCUS       DBNGVD010000547         4422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3059445, whole genome shotgun sequence.
ACCESSION   DBNGVD010000547 DBNGVD010000000
VERSION     DBNGVD010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3059445"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="AC16CM_14760"
     CDS             <1..2107
                     /locus_tag="AC16CM_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3141141.1"
                     /translation="VVRRLGGGRSRSRGGGGTEAGSGRGVEAKRPKTRQLDQYGRNLT
                     AYAEEDKLDPVIGRNQEIARIMQILVRRTKNNPVIIGEPGVGKTAIVEGLAEEIAEGK
                     VPEILGDKEVYTLDLGALVAGSKYRGEFEERLKKIMKEITDHGDIILFIDEIHNLVGA
                     GAAEGAIDAASILKPALARGEIQVIGATTIDEYRKYVEKDKALERRFQTIQVGEPSVE
                     ETELILKGLREKYEAHHNIDITDEALKAASQLGDRYIADRFLPDKAIDLVDEAASKMR
                     IKTMSSPPYYKEIDEELAAVRTEKEAAIDGQEFEKAARFRDSERKLVAQRRELEKAWR
                     EGKVEEKRVSIGEDEIAEIVSMWTGIPVKKMTEEESARLLQMEDALHGRIVGQNEAVK
                     SVSRSIRRTFAGIKDPNRPSGSFVFLGPTGVGKTELARTLAEYLFGNQESMIRLDMSE
                     YMERHTVSRLVGSPPGYVGYDEGGQLTEQVRRRPYSVVLFDEIEKAHPDVFNIMLQIL
                     EDGQLTDAQGRAVDFKNVVLIMTSNVGAQTINKTKSLGFGSDDAGLSYKEMKGRVTSE
                     LRKIFRPELLNRIDEIIVFHKLEREDMRHIIEIQIKRLRQQLVERDVTIEFTTEALDK
                     LAEDGYDPAFGARPLKRVLQRMIEDPMSEMILRGQVPNGSKVIIEPNDKSSDDINEEE
                     SIVDIKVTQPKEIVKVESE"
     gene            2260..3276
                     /locus_tag="AC16CM_14765"
     CDS             2260..3276
                     /locus_tag="AC16CM_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3141142.1"
                     /translation="MRLLVLGGGGFLGYHVVAEALRMGHVVSTFNREGEAPLDGVEAL
                     QGDREADLTALRGKSWDGVLDTFSDPDAVGETARLLSGAVGTYGYVSGISNYHPDGAQ
                     VVDEDAPLRGPEDDADDDSLQERSIAKIRCEEAVHRYFSGPVFTTRVGIMVGPRDPTD
                     RFSWWPERLARALAEGGEVLAPGDPRRPVQFSDARDVAAWMVGMLAAGTPGTYNAVGP
                     GREDPVAEVLDACRLAAGEEDGEELGPVRITWAEEGFLREQLREMPEEERPLWFPEDQ
                     IPFQKVDSSKALAAGLAFRPAYETARDTLAWVRSRPGGPELAAGFSAETEREILRNWH
                     AQRS"
     gene            complement(3296..3676)
                     /locus_tag="AC16CM_14770"
     CDS             complement(3296..3676)
                     /locus_tag="AC16CM_14770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR3141143.1"
                     /translation="MRPTVENVLFLHTVSIGTFGGSEGLRDLGSLRAAVARPWGSSFG
                     QDYFSTPFGKAAALAESLIRRHPFIDGNKRTAVYSAAYLLVTLGYELEAEQQDLEDFA
                     VSIALGEMGTTEIAYWFEEHSRKA"
     gene            complement(3679..3894)
                     /locus_tag="AC16CM_14775"
     CDS             complement(3679..3894)
                     /locus_tag="AC16CM_14775"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3141144.1"
                     /translation="MKKIGGSVALFIPPEMLEELRLESGTEVVVTSEGGNINVRRAAE
                     GPPDDLLEFAHRFTKKYETALRNLAQR"
     gene            4034..>4422
                     /locus_tag="AC16CM_14780"
     CDS             4034..>4422
                     /locus_tag="AC16CM_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HIR3141145.1"
                     /translation="MATKTLYVCSNCGYQAPKWLGRCPDCGEWSTFTEEVREAKKMVG
                     FAERATRQKARAAGRTLVLDEVPVVEGGGRMGTGVGELDRVLGGGIVPGSMVLVGGEP
                     GVGKSTLLLQVMGNLGEGCLMISGEESP"
BASE COUNT          884 a         1326 c         1468 g          744 t
ORIGIN      
        1 ggtcgtcagg aggctcgggg gcggccgtag ccgcagccgg ggcggcggtg gcaccgaggc
       61 cggcagcggg cgcggggtcg aggccaagcg tccaaagacc cgccagctcg accagtatgg
      121 caggaacctc acggcctacg ccgaggagga caagctcgac cccgtcatcg gcaggaacca
      181 ggagatagcg aggatcatgc agatcctcgt caggcgcacc aagaacaatc ccgtcatcat
      241 tggcgagccg ggcgtcggca agaccgccat cgtcgagggg ctcgcggagg agatcgcgga
      301 gggcaaggtg cccgagatcc tcggcgacaa agaggtctat acgctcgacc tcggcgcgct
      361 ggtcgccggc tccaagtaca ggggcgagtt cgaggagcgc ctcaagaaga taatgaagga
      421 gatcaccgac cacggcgaca tcatcctgtt catcgacgag atccataacc tcgtcggggc
      481 gggtgccgcc gagggcgcca tagacgctgc ctccatactg aagccggcgc ttgcgcgggg
      541 tgagatccag gtcatcggcg cgaccaccat cgacgagtac cgcaagtacg tggagaagga
      601 caaggccctg gagcgccgct tccagaccat ccaggtaggt gagccgtccg tcgaggagac
      661 ggagctaatc ctcaagggcc tgcgcgagaa gtacgaggcg caccacaaca tagacatcac
      721 cgacgaggcc ctgaaggccg cctcgcagct cggcgaccgc tacatcgcgg accgcttcct
      781 gccagacaag gccatcgacc tcgttgacga ggcggcctcc aagatgagga tcaagaccat
      841 gtcctcgccg ccttactaca aggagatcga cgaggagctc gccgctgtcc gcaccgagaa
      901 ggaagcggcc atcgacggcc aggagttcga gaaggccgcg cgcttcaggg actccgagcg
      961 caagctcgtt gcccagcgcc gcgagctcga gaaggcctgg cgcgagggca aggtcgagga
     1021 gaagcgtgtc tccatcggcg aggatgagat agccgagatc gtctccatgt ggacgggcat
     1081 cccggtcaag aagatgaccg aggaggagtc agcgcgcctc ctgcagatgg aggacgccct
     1141 gcacggccgc atcgtcggcc agaacgaggc cgtcaagtcc gtctcgcggt ccatccgccg
     1201 caccttcgcc ggcatcaagg acccgaacag gccgtccggc tcgttcgttt tcctcgggcc
     1261 aacgggcgtc ggcaagaccg agctggcaag gacccttgcc gagtacctct tcggcaacca
     1321 ggagtccatg atccggctcg acatgtcgga gtacatggag cgccacacgg tctcgcgcct
     1381 ggtcggctcg cctcctggat acgtcggcta cgacgaaggc ggccagctca cggagcaggt
     1441 caggcgtcgt ccctacagcg tcgttctctt cgatgagatc gagaaggccc acccggacgt
     1501 cttcaacatc atgttgcaga tcctggagga cggtcagctc accgacgcgc agggccgtgc
     1561 cgtggacttc aagaacgtgg tcctcatcat gacctccaac gtcggggcgc agaccataaa
     1621 caagaccaag tccctgggct tcggctcgga cgacgccggt ctctcctaca aggagatgaa
     1681 gggtcgggtc acgagcgagc tgcgcaagat cttccgtccc gagctcctga accggatcga
     1741 cgagatcatc gtgttccaca agctcgagcg cgaagacatg cgccacatca tcgagatcca
     1801 gataaagcgc ctccgccagc agctcgtaga gcgcgacgtg accatagagt tcacgacaga
     1861 ggccctggac aagctcgccg aggacggcta cgacccggcg ttcggcgcaa ggccgctcaa
     1921 gcgggtgctg cagcgcatga tcgaggaccc catgagcgag atgatcctgc gcggccaggt
     1981 tccaaacggc tcgaaggtga tcatagagcc aaacgacaag tcctcggacg acatcaacga
     2041 ggaagagtcg atagtggaca tcaaggtcac ccagcccaag gagatcgtca aggtcgaatc
     2101 cgagtaagga aataggaacg cgacgaggag ggtcgggacg aaagtcccgg ccctcctttt
     2161 gcgttgcaag cgtccggatg tgcaagcgct cacggcacgc aacctgtgtc cagggtaatc
     2221 gatagacggt gttcttcttt gtggtcgggg ggatctgact tgaggctgct ggtgttgggt
     2281 ggtgggggtt ttctcgggta ccacgtcgtg gccgaggcgc tgcggatggg gcatgtggtg
     2341 tcgaccttca accgtgaggg tgaggcaccc ctggatgggg ttgaggcgct gcagggcgac
     2401 cgagaagcgg acctgacggc cttgcgtggt aagagctggg atggcgtgct ggacacgttc
     2461 agcgatcccg acgcggtagg ggagacggcc cggttgctct cgggtgccgt cggcacctac
     2521 ggctacgttt cgggcataag caactatcac ccggacggag cgcaggtagt agacgaagac
     2581 gcgccgctgc gtgggcccga agacgacgcc gatgacgact ctttgcaaga gcggagcatc
     2641 gcaaagataa ggtgtgaaga ggcggtgcac aggtatttct caggacccgt ctttacgacg
     2701 cgcgtcggca tcatggttgg gccacgcgac cccacagatc gcttctcgtg gtggccggag
     2761 cgcctggcga gggccctggc ggagggcggc gaggtgctcg cccccggcga cccgcgacgt
     2821 cccgttcagt tcagcgacgc ccgcgatgtc gcggcgtgga tggtcgggat gctcgccgcc
     2881 ggaacccctg gcacctacaa cgctgtcggc cctggacgcg aggatcccgt cgccgaagtt
     2941 ctggacgcct gccgcctggc cgcgggtgag gaagatggtg aggagctcgg accggtccgg
     3001 ataacctggg ctgaggaagg cttcttgagg gagcaactcc gcgagatgcc ggaggaagag
     3061 cgcccgctgt ggttcccgga agaccagatc ccctttcaga aggtggactc ctcgaaggcg
     3121 ctcgccgccg gcctggcctt ccgtcccgcc tatgagaccg ccagggatac cctggcctgg
     3181 gtccgctccc gaccgggagg cccagagcta gccgccggtt tctcagccga gaccgagcgc
     3241 gaaattctac gaaactggca cgcgcaacgt agctagaacg acttctcctg cccattcagg
     3301 ccttgcggct gtgctcttcg aaccagtagg ctatctctgt ggtgcccatc tctcccaggg
     3361 ctatgctcac ggcaaagtcc tccaggtcct gttgctcggc ctccagttcg taacccaggg
     3421 ttaccagcag gtaagctgca gagtagactg ccgtcctctt gttgccgtcg ataaacggat
     3481 ggcgacggat caaagactcg gcgagggccg cggccttccc gaacggcgtg gagaagtaat
     3541 cctgcccaaa agacgagccc cagggacgcg ccaccgccgc acgaagagat cccagatccc
     3601 gtagcccctc cgagccaccg aacgttccaa tagagaccgt gtggagaaat agcacgttct
     3661 ctacggtcgg acgcaccgtc agcgctgtgc gaggtttcta agggccgttt cgtatttctt
     3721 cgtgaagcgg tgcgcgaact cgagcaggtc gtcgggcgga ccttctgccg cacgccgaac
     3781 gtttatgttg ccgccctcag atgtgaccac tacctccgta ccagactcca accgtagctc
     3841 ctctaacatc tccggcggga taaacagggc cacagaaccc ccgatcttct tcaagcgttt
     3901 cctggtttcc atcgtttacc tccagttgcg gcctgagtta aacatagttt aacttatctg
     3961 tctgaggaga tatagatagg ttgtcctacc ctttgtttgc tgtacttttg cggcgatcca
     4021 gtacaatcga gtggtggcta cgaagacgct atacgtttgc tctaattgcg gttatcaggc
     4081 gccgaagtgg ctggggcgtt gtccggactg cggcgagtgg agcaccttca cggaagaggt
     4141 gcgtgaggcg aagaagatgg tcggcttcgc ggagcgggcg acgcggcaga aggccagggc
     4201 cgctggcagg acgctcgttc tggacgaggt gcccgtcgtc gagggtggcg gcaggatggg
     4261 aaccggcgtg ggcgagctcg accgggtgct cggaggtggg atcgtgcccg ggtccatggt
     4321 cctggtcggc ggcgagcctg gggttgggaa gagcacgctc ttgctgcagg tgatggggaa
     4381 tctgggagaa gggtgcctca tgatctctgg tgaggagtcg cc
//
LOCUS       DBNGVD010000548         3278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_143616, whole genome shotgun sequence.
ACCESSION   DBNGVD010000548 DBNGVD010000000
VERSION     DBNGVD010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_143616"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="AC16CM_14785"
     CDS             complement(<1..488)
                     /locus_tag="AC16CM_14785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141146.1"
                     /translation="MQDLTVRRCEPEDYEGVYRVYSSPNAMAGTIGIPFSSPQEVREE
                     LASGGDGSFPLVACVGEEVVGQLTLSVYMNPRTRHSGHFGIAVRDDWQGKGVGTALME
                     ACLDLADNWLNLSRLDLRVYVDNAPAIALYERFGFEIEGTHKSFAYRDGEYVDAHVMA
                     RLR"
     gene            729..1715
                     /locus_tag="AC16CM_14790"
     CDS             729..1715
                     /locus_tag="AC16CM_14790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3141147.1"
                     /translation="MESRNLGSEGLVVSELDLGCMGMSEFYGTGDEEESLATIRRALE
                     LGIDFLDTADMYGPFTNEKLVGKAIGGRRDEVVLATKFGNVRGENGERLGISGDPDYV
                     RKACDASLERLGVEYIDLYYQHRVDPETPVEETWGAMKELVEAGKVRYLGISEAAPET
                     IRRAHTVHPVSALQTEYSLWSRDVEDEILPTVRELGIGFVPYSPLGRGFLTGAFSSPE
                     DFDEGDYRRNSPRFQGENFRRNLDLVDRVREIAQEKEVTAGQLALAWLLHQGEDIVPI
                     PGTKRREYLEENAAAADVELTGEDLRRIEEVAPAGAAAGERYDEAGMGAVNR"
     gene            1724..2113
                     /locus_tag="AC16CM_14795"
     CDS             1724..2113
                     /locus_tag="AC16CM_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3141148.1"
                     /translation="MAPEIRLDHCVVHVSDWERSNAFYRDVLGAELVGAGDRWSYRFG
                     DQQLNLHGPGVDADPVARIPVPPGGSDLCFVWPGNIEEAREHLGRHGVGVEMGPVGRR
                     GARGAGTSLYFRDPDGSLLEFISYESA"
     gene            2137..2736
                     /locus_tag="AC16CM_14800"
     CDS             2137..2736
                     /locus_tag="AC16CM_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HIR3141149.1"
                     /translation="MTDVEHISRKGTGVPVSWFPVPEESELPEGLRGLFRKARESVGF
                     VPNVFRAYSYRPERLSAWFAHYRQLHEPTDNLDAAEREMIAVAVSMANGCLYCLVAHG
                     AALREALGDPILADRITLDHRRAGLDERRTAILDFAVKITDRPLDCDPEDLQRLQSFG
                     LTEQEVWDVVEIASMYNFTNRMSLACGMIPNEEYHALAR"
     gene            2885..>3278
                     /locus_tag="AC16CM_14805"
     CDS             2885..>3278
                     /locus_tag="AC16CM_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3141150.1"
                     /translation="MEDPLDQAGNREGTTAASGSGSRLALKTEIVVIGAGQAGLSSAY
                     HLKKRGLMPHRGFVVLDQSSEAGGAWQFRWPSLRLSTVNRIHDLPGMRFSEAVDTDVT
                     EVEASVAVPRYFAAYEEAFDLPVYRPVKV"
BASE COUNT          617 a         1047 c         1070 g          544 t
ORIGIN      
        1 cgcagccggg ccatgacgtg ggcgtccacg tactcgccgt cgcggtaggc gaagctcttg
       61 tgggttcctt ctatctcgaa cccgaatctc tcgtagaggg ctatggcggg cgcgttgtcc
      121 acgtagacgc gcaggtccag ccgcgagagg ttcaaccagt tgtcggcgag gtcgaggcag
      181 gcctccatca gcgcggtacc gacgcccttt ccctgccagt cgtcgcgcac cgctattccg
      241 aagtgccccg agtgccgcgt cctcgggttc atgtagacgc ttagcgtgag ctggccgacg
      301 acctcttccc ccacgcaggc taccagggga aagctgccat cccctccgct ggccagctct
      361 tcgcgaacct cttgcgggga ggagaagggt atccctatgg tcccggccat ggcgttcggg
      421 gatgagtaga cgcggtagac accctcgtag tcttccggtt cgcagcggcg aacggtcaga
      481 tcctgcaagc agtccctcct tcttccggca cgttatcttc gcataaccat ccccgaccct
      541 ttcgtagtgg tgtgttatct agagtggcct tacggaaaga caaagccgag aatgtcgaag
      601 gcgacgggca tcagcaaacc acctgaagcc tgttgcctga aacctgaagc cttgtgtaag
      661 aaacgcccgc ggttgggtac gacagagcag agaacatatt tgtagaagca agtttttggg
      721 agacatttat ggagagcaga aatctgggta gcgagggcct cgtggtatcc gagctcgacc
      781 tcgggtgcat ggggatgagc gagttctacg gtacgggcga tgaggaagag tcgctcgcca
      841 ccatccgtcg ggcgctggag ctcggcatag acttcctcga cacggccgac atgtacggcc
      901 cgtttaccaa cgagaagctc gtcggcaagg ccattggggg caggcgcgac gaggtcgttc
      961 tcgccaccaa gttcggcaac gtgcgtggcg agaacggcga gcggctaggc ataagcggcg
     1021 accccgacta cgtgagaaag gcctgcgacg cctcgctgga gcgccttggg gtcgagtaca
     1081 tagacctcta ctaccagcac cgcgtcgatc ccgagacgcc cgtcgaggag acctggggcg
     1141 cgatgaagga gctagtggag gccggaaagg tgcgctacct cggcatctcc gaggccgcgc
     1201 ccgagaccat caggcgggcc cacaccgtgc accccgtcag cgccctgcag acagagtact
     1261 cgctgtggag ccgcgacgtg gaggatgaga tccttcctac cgtcagggaa ctcgggatcg
     1321 gcttcgtccc ctacagcccg ctcggacgag gttttcttac gggtgccttc tcgagccccg
     1381 aggacttcga tgagggcgac taccggcgca actcgccgcg cttccagggc gagaacttca
     1441 ggagaaacct ggacctcgtc gacagggtga gggagatcgc ccaggagaaa gaggtaaccg
     1501 cgggacagct cgccctcgcg tggctgctcc accagggcga ggacatcgtt cccatccccg
     1561 gtaccaagcg ccgcgagtac ctggaggaga acgccgctgc cgctgacgtc gagctgaccg
     1621 gcgaggatct gcggcgcatc gaagaggtgg cccccgccgg cgccgccgcg ggcgagcgat
     1681 acgacgaggc gggcatgggg gccgtcaacc gctagggagg accgtggcac cggagataag
     1741 gctcgaccac tgcgtggtcc acgtctcgga ctgggagagg tccaacgctt tctaccggga
     1801 cgtgctcggc gcggagctgg tgggggcagg agacagatgg tcgtaccggt tcggcgacca
     1861 gcagctcaac ctgcacggcc ccggcgtcga cgcggacccg gtcgccagga tacccgtgcc
     1921 accgggcggc agcgacctct gctttgtgtg gccaggaaac atcgaagaag cccgcgagca
     1981 cctcggtcgg cacggcgtcg gggtagagat gggcccggtc gggcgccgcg gcgcacgagg
     2041 ggcaggaacg agcctctact ttcgcgatcc cgacgggtcg ttgctagagt tcatctcgta
     2101 cgagagcgca tagagccggc gcagactgga ggccccatga ccgacgtaga gcacatctcg
     2161 cgcaagggaa ccggcgtccc cgtaagctgg ttccccgtcc ccgaggagtc agagttgccc
     2221 gagggtttac gggggctctt ccgcaaggcg cgcgagagcg tcgggttcgt gccaaacgtc
     2281 ttcagggcct acagctatcg tcccgagcgg ctctccgcgt ggttcgccca ctacaggcaa
     2341 ctccacgagc cgacggacaa cctcgacgcg gcggagaggg agatgatcgc cgtggccgtc
     2401 tcgatggcga atggctgtct ctactgcctg gtagcccacg gcgccgctct gagggaagcc
     2461 ctcggcgacc ccatcctcgc cgaccgcatc acgctggatc acaggagggc cggccttgac
     2521 gagcgccgca ccgccatcct ggacttcgcc gtcaagatca ccgatcgacc cctggattgc
     2581 gaccccgaag acctccaacg cctccagagc ttcggcctta ccgaacaaga agtgtgggac
     2641 gtggtagaga tcgccagtat gtacaacttc acgaaccgca tgtccctcgc gtgcggcatg
     2701 atccccaacg aggaatacca cgccctcgcc aggtgaccgt cacccgggca acgagctgac
     2761 tcctaccatc cgcctcccca tgtaagagga tcgttggtaa tgttgctcca gcgagattcg
     2821 ggtcttcggc tcaacgggcg ccgcgctccc cgcctcttgg caaccctata gaatacgcaa
     2881 gacgatggaa gaccctttgg accaggccgg caaccgcgag gggaccacgg ccgcctcggg
     2941 atctggctcg cgcctcgcac tcaagacaga aatcgtcgtc atcggggccg gccaggccgg
     3001 cctctcctcg gcgtaccacc tcaaaaagcg tggtctgatg ccgcacaggg ggttcgtcgt
     3061 gctggaccag tcgtccgagg cgggcggtgc ctggcaattc cgctggccct cgctgaggtt
     3121 gagcaccgtc aaccgtatcc acgatctgcc cgggatgcgc ttctccgagg cggtggacac
     3181 cgacgtgacg gaggtggagg cgagcgtcgc ggtaccgagg tatttcgcgg cctacgagga
     3241 ggccttcgac ctccccgtgt accggcccgt gaaggtga
//
LOCUS       DBNGVD010000549         2557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2697091, whole genome shotgun sequence.
ACCESSION   DBNGVD010000549 DBNGVD010000000
VERSION     DBNGVD010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2697091"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /gene="mraW"
                     /locus_tag="AC16CM_14810"
     CDS             <1..455
                     /gene="mraW"
                     /locus_tag="AC16CM_14810"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HIR3141151.1"
                     /translation="RVAREVIRRRPLQTTTDLYDAVRAAVGWKERGGNPAKRVFQAIR
                     IEVNDELGSLARALEATERLLVPGGRLVVITFHSGEDRLVKRFIAERQGRCVCPPELP
                     VCVCGARPIFRRGAVMRPSEREVGENPRSASARLRVAIRTDEAPEGGG"
     gene            590..895
                     /locus_tag="AC16CM_14815"
     CDS             590..895
                     /locus_tag="AC16CM_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141152.1"
                     /translation="MLVVVPVLLMLGSVYLHTVSAGLDIRAAELGVRLDRVQDEGERL
                     QVKVTELSGSARIRELAGKFGMRQPQTDSLRVYGDEPRGNAEDGIQDGTQKKGGEKR"
     gene            1035..>2557
                     /locus_tag="AC16CM_14820"
     CDS             1035..>2557
                     /locus_tag="AC16CM_14820"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3141153.1"
                     /translation="MPSSASSRASAGSGGGRTVGHRRLWTVAAVFLISGLLLGGRAVH
                     ISLTEDQDYRAFAAEQSWDGIQSAPPMRGSIVSADGRELATSLEVARVIATPYQVVDP
                     RATAGKLKSLMGSETDRSVDDIEAALTRRDSNDQLMGYSVVATGIKPQTAEKIQGAAF
                     EGITIAPDAERVYPNGSLASQVVGHAGDYGQAFGGVEERFDGRLKSGKDVDLTVDSAV
                     QQELQKALATAVKKSDAKSAVGVLMRVDDGSIVALANTPSYDNNNFDEVPVEDQRNKA
                     LTDTYEPGSTFKAFTVASALEEGAITPDEEFVVPDHMVVADHVINDSEPHETEVLTPG
                     GVLKHSSNVGATKIAMKMGGKNLYDRMVSFGFGQPTGVDLWGEDPGILPAYKDWSGIS
                     IGNIPFGQGFTVSPIQLTSAYATLINGGYKVEPHVAEGDAPAEHGPRVISGETSAIVR
                     GMLQGVVDDGSGHFAQISGFTVGGKTGTSQKVDPKTGTYGGGYVASFVGFAPVEDPE"
BASE COUNT          489 a          801 c          897 g          370 t
ORIGIN      
        1 gcagggtggc acgggaggtc ataaggaggc gaccgctaca gaccacgaca gacctttacg
       61 acgccgtgcg tgcggctgtg ggttggaagg aacggggcgg taaccctgcc aagagggtct
      121 tccaggccat ccgcatcgag gtgaacgacg agctcgggag cctcgcgagg gccctcgagg
      181 ccaccgaacg gctgctcgtg cctggaggca ggctcgtggt catcaccttc cactcggggg
      241 aggacaggct ggtcaagcgt tttatcgccg agcggcaggg acggtgcgtg tgccccccgg
      301 agctacccgt ctgcgtctgc ggcgcgcgcc ccatcttccg gcggggtgct gtcatgaggc
      361 catcggagcg cgaggttggg gagaacccgc gcagtgcctc ggctcggctg cgggtggcca
      421 taaggacgga cgaggcgccg gagggtggtg gctgatggca gcgccgcagc gcaggtacag
      481 ggctacgctt cccaccgaac cggcgtggcc agcccccgga cgtgccccgc gcaggtctcc
      541 ggagcgcgcg ctgcgaaaga aagccgcagg ccgccggcgg cggtttgcaa tgctcgtggt
      601 ggtgcccgtg ctgttgatgc tggggagcgt ctacctgcac acggtctccg ccgggctcga
      661 tatcagggct gccgaactcg gcgtacggct cgatcgggta caggatgagg gggagaggct
      721 ccaggtcaag gtcaccgagc tgtctggctc ggccaggatc agggagcttg ccggcaagtt
      781 cggcatgcgc cagccgcaga ccgactctct gagggtctac ggagatgagc caagaggtaa
      841 tgcggaggat gggatacagg atggcacaca aaagaaggga ggggagaagc ggtagagccg
      901 aaggccccag gcgtcgcaac gccggacgcc ggcgcgccag gactaccccc cctcgcagca
      961 gagcccggga ccatcagggt ccgggcggca aggtcataca cctctcggga cggaggcgta
     1021 cgaagggcag acgtgtgcct tccagcgcgt cctcccgggc ctccgccggc tctggtggcg
     1081 ggcggaccgt gggccacagg cggttgtgga ccgtggccgc ggtcttcctg atcagcgggc
     1141 tgctactcgg tggtagggcc gtgcacatca gcctgacgga ggaccaggac tacagggcct
     1201 tcgccgccga gcagagctgg gacggtatcc agagcgcccc gccgatgcgc ggcagcatcg
     1261 tcagcgccga cggcagggag ctcgcgacca gcctcgaggt ggcgcgtgtg atcgccaccc
     1321 cctaccaggt cgttgacccg cgtgccacgg ccgggaagct caagtctctt atgggttctg
     1381 aaacggacag gagcgtggac gacatcgagg ccgcgctcac cagacgcgac tcgaacgacc
     1441 agctgatggg gtacagcgtg gtagcaaccg gcataaagcc ccagacggct gaaaagatac
     1501 agggagcggc attcgagggc atcaccatcg cccccgacgc ggagcgggtc tacccgaacg
     1561 ggtcgctcgc cagccaggtc gtcggccacg ccggcgacta cggccaggcc ttcggcgggg
     1621 tcgaggagcg cttcgacggc aggctcaaga gcggcaagga tgtagacctg acggtcgatt
     1681 cggcggtgca gcaggaactt caaaaggccc tagccaccgc ggtaaagaag agcgacgcca
     1741 agagcgccgt aggggtcctc atgcgggtgg acgacggttc catagtggcg ctcgcgaaca
     1801 caccgtctta cgacaacaac aacttcgacg aggtgccggt cgaggaccag cgcaacaagg
     1861 cactcaccga tacctacgag ccaggctcaa ccttcaaggc cttcaccgtg gcctccgccc
     1921 tcgaggaagg cgctattacc cccgacgagg agttcgtcgt ccccgaccac atggtggtcg
     1981 cggatcacgt aataaacgac tccgagccgc acgagaccga ggtactcaca cccggtggtg
     2041 ttctgaagca ctcgagcaac gtgggggcca caaagatcgc catgaagatg ggcggtaaga
     2101 acctctacga ccgcatggtc tccttcggct tcgggcagcc tacgggcgta gacctctggg
     2161 gtgaagaccc ggggatcctc cccgcctaca aggactggag cggcatctcc ataggcaaca
     2221 tacccttcgg gcagggcttc accgtgtcgc cgatccagct gacctcagcc tacgccacac
     2281 tcatcaacgg cggctacaag gtcgagccgc acgttgccga gggagatgcc cccgccgagc
     2341 acggcccccg ggtgataagc ggcgagacct cggctatagt acgcgggatg ttgcagggcg
     2401 tcgtcgacga cgggtctggc cacttcgccc agatttccgg tttcacggtg ggcggcaaga
     2461 cgggcacctc gcagaaagtc gacccgaaga cgggaaccta cggcgggggg tacgtggcct
     2521 ctttcgtcgg gttcgccccg gtggaagacc cggagta
//
LOCUS       DBNGVD010000550         2396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3424222, whole genome shotgun sequence.
ACCESSION   DBNGVD010000550 DBNGVD010000000
VERSION     DBNGVD010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3424222"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC16CM_14825"
     CDS             <1..848
                     /locus_tag="AC16CM_14825"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3141154.1"
                     /translation="PLVDVEVITLLYAIHNAVGVKDEIVHLNNLGDAETRNSYVPQLR
                     AYLQRHSDELDPDSLARMDTNPLRTFDSKHEGTQAVLAGAPRISSFLSDEASAHLEAV
                     REGLAALGVPYEIDDGLVRGLDYYTMTVFEAKSGALGAQDTVGAGGRYNGLISDLGGP
                     DVGGIGFGSGVERMLLAAASQEQQPGLDVFFVTLSEEARIPAMRLAGALRGEGISADL
                     DFGGRGMRAQFKQADRAGATYAVILGEDELAQGVLTVRNMASGEEQKVPAAEGPKNLL
                     RAVTG"
     gene            852..1586
                     /locus_tag="AC16CM_14830"
     CDS             852..1586
                     /locus_tag="AC16CM_14830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3141155.1"
                     /translation="MEGPAPGLVSALRNSSHTVVLTGSGISAESGVPTFREAQTGLWE
                     RFDPQELATPEAFDRDPGLVWNWYAWRRELVARAVPNPGHLALVEMQDLVPSLTVVTQ
                     NVDGLHQRAGSRGVIELHGNILRTRCSAEGIEVDGYEASELPPVCPSCGAPLRPDVVW
                     FGEMLPPEALDAASEAAQGADIFLSVGTSSLVYPAAALPYEALEGGATLVEVNPEQTP
                     LTPHAEHVLRGSAGEHLPRLLRELRE"
     gene            1656..2042
                     /locus_tag="AC16CM_14835"
     CDS             1656..2042
                     /locus_tag="AC16CM_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141156.1"
                     /translation="MPVEDLRQSPMMAHMLDALDNGEDIGHYGRLVFAMIGRHFVSND
                     ELVELLTQDHDADEQEIRAMVQQVEDKGYSPPRREKILEFQDQQDFAICPNPDDPDAC
                     NVYNELQFPDDVYESIQQYQEKRQTS"
     gene            2092..>2396
                     /locus_tag="AC16CM_14840"
     CDS             2092..>2396
                     /locus_tag="AC16CM_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3141157.1"
                     /translation="MKARITVITIGVDDLERSLRFYRDGLGLSTQGIIGEEFEHGAVA
                     FFELQAGLTLALFPRASIARDSTVPEGPSSPTEFTLGHNVSSRDEVDEIMQQAREAGA
                     "
BASE COUNT          428 a          775 c          805 g          388 t
ORIGIN      
        1 atccgctcgt agacgtcgag gtgatcacgc tgctctacgc catccacaac gcggttggtg
       61 tgaaggatga gatcgtgcac ctgaacaacc tgggcgacgc cgagacgaga aactcttacg
      121 tgccccagct gcgggcctac ctgcagcggc acagcgacga gctagacccc gactcactgg
      181 cccgcatgga cacaaacccc cttcgcacct tcgactccaa gcacgaggga acccaggccg
      241 tgctggcggg ggcccccaga atctcctcgt tcctgagcga cgaagcctcg gcgcacctgg
      301 aggccgtgcg cgagggtctc gccgcgctcg gcgtaccgta cgagatcgac gacgggctcg
      361 tgcgtggcct cgactactac acgatgaccg tgttcgaggc gaagagcggg gcgctcgggg
      421 cgcaggacac cgtcggggcc gggggacgct acaacggcct gatctcggat ctgggggggc
      481 cggacgtggg ggggataggt ttcggctccg gggtggagcg catgctgctg gccgccgctt
      541 cgcaggagca acagccgggt ctcgacgtgt tcttcgtcac cctctccgag gaggcccgca
      601 tccccgccat gcggctcgct ggtgctctgc ggggggaggg catctccgcg gacctcgact
      661 tcggggggcg tgggatgcgg gcgcagttca agcaggcaga ccgtgccggt gcaacctacg
      721 ctgtcattct gggcgaggac gagctggccc agggcgtgct cacggtgcgt aacatggcct
      781 cgggcgagga gcagaaggtg ccggcggcgg agggtcccaa gaacctgctg cgcgccgtga
      841 ccggctagac gttggagggc ccggcgccag gcctggtctc cgccctgcgc aattcttccc
      901 acacggtcgt tcttaccggc tccggtatct cagccgagag cggcgtgccg actttccgcg
      961 aggcccagac cgggctctgg gaacgcttcg atccgcaaga gctcgccacc ccggaggcct
     1021 tcgaccgaga ccccgggctg gtatggaact ggtacgcgtg gcggcgggag cttgtcgcgc
     1081 gggcggtgcc caacccgggg caccttgccc tcgtcgaaat gcaggatctc gtcccgagcc
     1141 taaccgtcgt tacccagaac gtcgatggtc tgcaccagcg tgccggcagc aggggcgtga
     1201 tcgagcttca cggcaacata cttcggacgc ggtgttcggc ggagggcatc gaggtcgacg
     1261 ggtacgaggc gagcgagctg cccccggttt gtccgtcctg cggtgccccc ctgaggcccg
     1321 acgtggtctg gttcggcgag atgctgccgc ccgaggcgct ggacgcggca tcggaagcag
     1381 cgcagggcgc ggacatcttc ctctccgtcg gcacctccag cctcgtctat cctgccgcgg
     1441 cgttgcccta cgaggcgctc gagggtggcg cgaccctcgt ggaggtaaac ccggaacaaa
     1501 ccccgctcac gccgcacgcc gaacacgtgt tgcgtgggtc ggcgggtgaa catctaccgc
     1561 gcctcttgcg ggagctcagg gagtagtcgt gttcacgttc tgaaccgatc cagcgcgtat
     1621 atttcgggca gtatccaccc gagaggagca caccgatgcc cgtagaagac ttgcgccaga
     1681 gcccgatgat ggcccacatg ctagacgcgc tcgataacgg cgaggacatt ggccactacg
     1741 gccgtctcgt gttcgccatg attggccgcc atttcgtctc caacgacgag ctcgtggagc
     1801 ttctaacgca agaccacgac gccgatgagc aggagatccg ggccatggtg cagcaggtgg
     1861 aggacaaggg ctacagcccg cccaggcgcg agaagatcct cgagttccag gaccagcagg
     1921 acttcgctat ctgccccaac cccgacgatc cagacgcgtg caacgtgtac aacgagctac
     1981 agttccccga cgatgtctac gagagcatcc agcagtacca ggagaagcgc cagacgtcct
     2041 agcaggtgtg gctccgtacc gcgaagtaca cgtggataac ttgggagaag catgaaagcc
     2101 cgcatcaccg ttatcaccat cggcgtcgat gacctcgaga ggtcgctccg tttttaccgg
     2161 gacggcctcg ggctctccac gcagggcata ataggggaag agttcgagca cggggccgta
     2221 gccttcttcg agctgcaagc cggcctgacg ctcgcgctct tcccgcgagc gagcatcgcc
     2281 cgggattcca cggtacccga agggccttcg agtccgacgg agttcacgct cgggcacaac
     2341 gtctcctcca gggatgaggt cgatgagatc atgcagcaag ccagggaggc gggggc
//
LOCUS       DBNGVD010000551         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3606739, whole genome shotgun sequence.
ACCESSION   DBNGVD010000551 DBNGVD010000000
VERSION     DBNGVD010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3606739"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16CM_14845"
     CDS             <1..320
                     /locus_tag="AC16CM_14845"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR3141158.1"
                     /translation="PNHKAMPLLEQDSEVDFLRRQVLTSRNVLAHPITDFWYGGLNYQ
                     IEHHLFPRMPRNKLKEAQPIIKGFCRRHDISYHETSVLQSYREILQHLHEVGAPLRKR
                     SKV"
     gene            657..1802
                     /locus_tag="AC16CM_14850"
     CDS             657..1802
                     /locus_tag="AC16CM_14850"
                     /inference="COORDINATES: protein motif:HMM:NF017856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR3141159.1"
                     /translation="MEAREGTSRRARAVATARARLRAGWIRVRSGWLQIIQTALAACV
                     AWILSVLILGVERPTFAPIAAVIALGLTLGKRGRRAVELTLAVAFGVALADLLLSVLG
                     VGAIQAGFFVALAMAVAVFLGAGNLGVNEAAISAMIMMFTFQPSAAGLPIERFLEALI
                     GGGTALLINALLPVNPERLVEDAAFPVFAESAAVLESVADSLASCDAGLVQRAYVKAR
                     EIDARVAGLKEAVAAGRETARLAPPRRGSLGHMQLYASAADQIDLTVRDVRALARAAL
                     SVVQPGDPAPEPLPAAIRGLARATEALAAYLQTSGDPDETRRLALGASTEASTMLEEH
                     EDLARNLNVNALVDQIHSSAVDLVGGTGLDRAAALQALQEATGRASW"
     gene            complement(2000..2272)
                     /locus_tag="AC16CM_14855"
     CDS             complement(2000..2272)
                     /locus_tag="AC16CM_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141160.1"
                     /translation="MGALIGALVVLLVITMGAGATALVVVGGGVGYFVGKFLDGELDL
                     EEVRARAQGRDQGRDTRAQEDGPYAPPAPVASGAGSPSRSTPLSVR"
     gene            complement(2720..3151)
                     /locus_tag="AC16CM_14860"
     CDS             complement(2720..3151)
                     /locus_tag="AC16CM_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3141161.1"
                     /translation="MSKQRSEEQNKNPLQSDHGNTTIQDSVVSKIAGIAAQEVDGVRM
                     GSGASQSVGGVLDTITGGRAGASQTSGVSVEVGQEEAAIDLTMMVEYGKPIPQLAEAV
                     RNNVTSRVERLVGLRVTEANITVQNVFFPGEEDQGQQQAER"
     gene            complement(3398..3556)
                     /locus_tag="AC16CM_14865"
     CDS             complement(3398..3556)
                     /locus_tag="AC16CM_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141162.1"
                     /translation="MASIGTKIKEAAMKAAKEKVSGDDKKDTKDKRTAGSESGMDKVM
                     RAVKNRLK"
BASE COUNT          599 a         1203 c         1115 g          684 t
ORIGIN      
        1 cccccaacca caaggccatg ccccttctgg agcaggacag cgaggtggac ttcctccgcc
       61 gccaggtgct gaccagccgc aacgtcctgg cacacccgat caccgacttc tggtacgggg
      121 gcctcaacta ccagatagag caccacctct tccccaggat gccccgcaac aagctcaagg
      181 aagcgcagcc gatcatcaag ggcttctgcc ggcggcacga tatctcctat cacgagacga
      241 gcgtgctcca gtcctaccgc gagatcctgc agcacctgca cgaggtcggc gcccccctac
      301 gtaaaaggag caaggtgtag tggcggctcg ggtagagcca cagcgagaac ccagaccgag
      361 cgcgaattgc agagggatca gcacctctgc aattcgggcc gtcagctatc ggcttaatga
      421 acttgctgtc aactgaatgt tgataacgtg ccaagtagtt gaaccccctg gcaatctgct
      481 ctgagaggtg caggcgcgaa gatgggagcg aacgaacccg aacgggggag gcgcctggct
      541 agcgacaaaa gggcggacga aggtacggaa gggagcgcag cagattaggc acgaagagcg
      601 ggaaccccgg ttcggcggac cgcgcgtagt cgggcagtaa agaataggag tgcgggatgg
      661 aggcgcgcga aggtacgtcc agacgggccc gggcggtcgc gaccgcccgg gcccgtctgc
      721 gcgccggctg gatccgtgtg cggtccggtt ggttgcagat catccagacg gcgctcgcgg
      781 catgcgtcgc gtggattctg tctgtcctca tcctcggggt agagcggccc acgttcgcac
      841 cgatagcggc ggtgatcgcg cttggactca cgttgggcaa gcgcggcagg cgcgccgtgg
      901 aactcaccct cgcggtggct ttcggggtcg cactcgccga ccttctcttg tctgtactgg
      961 gcgtgggagc tatacaagcc gggttctttg tggccctggc gatggcggtg gccgtgttcc
     1021 ttggagcggg gaatctcggc gtgaacgagg ccgccatctc ggcgatgatc atgatgttca
     1081 cctttcagcc ttccgccgcc ggcctgccta tagagcgctt cctggaggcc ctgatcggtg
     1141 gtggcaccgc attgcttatc aacgcgctgc tcccggtcaa ccccgagcgc ctggtcgagg
     1201 acgccgcgtt tcctgtcttc gccgagtctg cggcggtgct cgagagcgta gcggattccc
     1261 tggcgagctg cgatgccggg ctggtccaga gggcgtacgt gaaggccagg gaaatagacg
     1321 cgcgggtcgc cgggctaaag gaggcggtcg ctgccggtcg ggagaccgcg cgcctcgccc
     1381 cgccccgtcg cgggagcctg gggcacatgc agctctacgc cagtgcggcc gaccagatag
     1441 acctgaccgt gcgcgacgtg agggcgctgg cccgcgccgc gctctccgtg gtccaacctg
     1501 gagaccctgc cccagaacct ctgcccgctg cgatccgggg ccttgccagg gcgacggagg
     1561 ccctggccgc ctacctgcag acatcaggag atcccgacga gacgcgccga ctggccctgg
     1621 gcgcctccac agaggccagc acgatgctcg aggagcacga ggatctggcg aggaacctca
     1681 acgtcaacgc gctcgtggac cagatccact cctcggcggt cgatctcgta gggggcaccg
     1741 gcctggatcg ggcagcggcc ctgcaagccc tgcaagaagc caccggacgc gcctcgtggt
     1801 agccagcgcg ctaccggata gccgtccggt gcgcgcaggt cgggaaccgc tcaggcagcg
     1861 ggtcctggct cctatttacc gaagggcatg gaagtagagt ctgcaacctt tgtggttgcg
     1921 aaccgtgtag gagccttgga catagcgcgg tacgaccacc tcgacccgcg tgtgcccctt
     1981 gccgtgcctg ccggacccct taccggacgc tcagtggcgt gcttcgtgaa ggagaacccg
     2041 cccccgaggc taccggcgca ggcggagcgt agggaccatc ctcctgtgct cgggtgtccc
     2101 gtccctggtc gcgcccctgg gcgcgagcac gcacctcttc gaggtcgagt tccccgtcca
     2161 ggaacttgcc aacgaagtag ccgacgccgc cgcccaccac gaccagcgcg gtggccccgg
     2221 cgcccatcgt gatcaccagg agcacaacta gcgccccgat caaagccccc accgcttgtt
     2281 tgtccaggtt tccatgaggt acttttctac ttgagttgcg gtttctttga cggtgacctc
     2341 aacgttcttt acggacacgt tctgagtgta gagcaccttc gagatgttct ccctggcgcg
     2401 ggccgcgagc ccggtaaggt cgctcgaggt cggggctccg acggcgcatg agacgctata
     2461 ggcacccttt gccgggctca gggcaacctt cggggaagta gccccagcct cccgggcggc
     2521 accctcgacg agtgcgcgtg cggctcttcc tgctccttag gtcgcggcct cgacgtagcg
     2581 gcccgcaccc atcgagtgaa ctccatcggt cgagagggca gccgccgctg cggcggctgc
     2641 aagccttagt tcttcgttca cggtcccagc caactcttga tatcgtgttt ccctaccgga
     2701 ccctgctccg ccgctgccct tagcgttctg cctgctgttg gccctggtct tcctctcccg
     2761 ggaagaagac gttctggacc gtgatgttgg cttccgtgac ccgcaggccg accagcctct
     2821 cgacccggct cgtgacgttg ttgcgcacgg cctcggcgag ttgggggata ggcttgccgt
     2881 actccaccat catcgtcagg tcgatggccg cctcttcctg gccgacctca accgagacgc
     2941 ccgacgtttg acttgcgccg gccctgccgc cggtgatagt gtcgagaacg ccgccaacgg
     3001 actgcgaggc gccgctgccc atcctgacgc cgtcgacctc ctgggcggcg atgccggcta
     3061 tcttggagac cacactatcc tgtatcgtgg tgttgccgtg atcgctctga agcgggttct
     3121 tgttctgctc ctcgctcctc tgtttgctca ttgccacgac tcctttcgtt cgacttgctc
     3181 gacacaacgc caacctggcg attacgcttg ggccggctcc gcaagaacct gatccctaga
     3241 gcatctgcag tacgagcaat accaccacgg ttaccacggc gacgattatc aacgtgcgtg
     3301 ccatcactca cctcctctcc cagccaggtt ctccaccaag cgtgataaca gaaagaccgg
     3361 gaccccgtgg agtcccggcc cgacacagca tgctcctcta cttcagacgg ttcttgacgg
     3421 ctctcatgac cttgtccatg ccgctttccg agcctgcggt gcgcttgtcc ttcgtgtctt
     3481 tcttgtcatc accggagact ttctcttttg cggccttcat cgctgcttct ttgatcttcg
     3541 tgcctatgct ggccatgtgt gccacccttc tcttgggcct acgtctctat ccatctacga
     3601 a
//
LOCUS       DBNGVD010000552         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1967879, whole genome shotgun sequence.
ACCESSION   DBNGVD010000552 DBNGVD010000000
VERSION     DBNGVD010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1967879"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="AC16CM_14870"
     CDS             complement(<1..1039)
                     /locus_tag="AC16CM_14870"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3141163.1"
                     /translation="MAQENVLIIGAGPSGIFAASELARHGVTARLVEQQVRPHHEARA
                     TAIQPGTLEILDSVGLLEPFLQSAEHVHRVRVYGPDMSELAALDFEGIDCRCEFQCSL
                     PQYETQRILEAHLASLGGSIGRGVTATKVETDADDVSVELAHEDGTLETVRPDFLIGA
                     GGAHSVTRRSMSESLEGTTYQGHFLVADVAMEAPVPRDQGNFFCGPSGMMLLAPLPGG
                     RWLTFQDLEETAQTVSVEDVVARTEARIGGRSRPTDVAWVSPFRMHRRIVSRLADGRR
                     FLIGDAAHLSSPFGGEGLNSGLHDGYDLAWKLALVLSGDAHRSLLDDYAVEREIADRH
                     VLDVSDDVHTGI"
     gene            complement(1141..2223)
                     /locus_tag="AC16CM_14875"
     CDS             complement(1141..2223)
                     /locus_tag="AC16CM_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141164.1"
                     /translation="MERDKSWSGHEPEAAAGGREKSPPASENRTVPEGMTAEPGVGQD
                     SREHLVGLLGAYQETLDEVGALPGVVWLPWARKGPGSQVKVPYLRWFLTYFVTHHINR
                     NLNALNRRYHAAAALADDPESNWKARRAVELFLRSLPSPPYRVLMVTAVLTALVIALP
                     LQSFGNVFYLLDLVAALLRFDAGYIGEALEGGQQVGPAVRSVMVLLVGLTVVAAVLTS
                     PFGLKRILFNLHPRTDETLESTAAREHVYRVEGVYALEQRVFEEAGIRRPREGLWDLA
                     FLTFLLLLVLVVGLCLALLTLVILKGWSIDINVETGAPGRFQFYLPQVSWFYYGLFTA
                     LIFATFVLLLRRLIMAWKKRVWHAGG"
BASE COUNT          449 a          844 c          797 g          411 t
ORIGIN      
        1 ttatcccggt gtgtacgtcg tcggagacat cgaggacgtg acggtcggcg atctcgcgct
       61 ccaccgcgta gtcgtctagc agcgaccggt gtgcgtcgcc gctcaggacg agcgccagct
      121 tccacgcgag gtcgtagccg tcatgcagcc ccgagttcag cccctcaccc ccgaacgggc
      181 tggagaggtg ggcggcgtca ccgatgagga accgccggcc gtccgccagg cgcgagacga
      241 tccgccggtg catccggaaa ggcgagaccc atgcaacgtc ggtgggcctt gaccttccgc
      301 cgatccgtgc ctcggtgcgg gcgacgacgt cctcgacgga taccgtctgt gccgtctctt
      361 ccaggtcctg gaaggtcagc cagcgcccgc cgggcaatgg cgccagcaac atcatgccgc
      421 tcgggccgca gaagaagttg ccctggtcgc gcgggacggg ggcctccatg gcgacatcgg
      481 cgacgaggaa atggccctgg taggtggttc cttcgagcga ctcgctcatc gaacggcggg
      541 tgacgctgtg cgccccgccc gccccgatca ggaagtccgg cctgaccgtc tcgagggtac
      601 cgtcctcgtg cgcgagctcg acggagacgt cgtcggcatc cgtctccact ttcgtcgccg
      661 tgacccccct cccgatcgag ccgccgagcg acgcgagatg ggcctcgagg atccgctggg
      721 tctcgtactg cgggaggctg cactgaaact cgcagcggca gtcgatgccc tcgaaatcca
      781 gcgccgcaag ctcggacatg tcagggccgt acacacgaac gcggtggacg tgctcggcgc
      841 tctggaggaa aggttcgagc aggccgacgg aatccaggat ctccagggtg ccaggctgaa
      901 tcgccgtggc acgcgcctcg tggtgtggcc gcacctgctg ctccacgagc cgcgcggtta
      961 cgccgtggcg ggcgagctcg gaggcggcga aaatccccga gggtccggcg ccgatgatca
     1021 ggacgttctc ttgcgccatt atcctcctcc agaagccagc caccaggcat ggaagtctag
     1081 caaaccacca aatctggagc taccgcgaaa ctcgtcggtc ggcggcgttg accgcctgcc
     1141 tcaaccgcct gcgtgccaga ctcgtttctt ccaggccata attagccttc tgagaaggag
     1201 cacgaaggtg gcgaagatca gggcggtaaa cagcccgtag taaaaccagg atacctgcgg
     1261 caagtagaat tggaatcttc ccggtgctcc ggtttccacg ttgatgtcga tgctccaccc
     1321 cttcaggatg accagcgtca ggagcgccag gcagagccca accaccagga ccagcaggag
     1381 caggaaggtg aggaaggcga gatcccagag tccctcccgc gggcgccgga tgccggcctc
     1441 ctcgaaaaca cgttgttcca gggcgtagac gccttccacc ctgtagacgt gttcccttgc
     1501 cgcggtcgat tccagggttt cgtcggtccg cggatgcagg ttgaagagta tgcgcttcag
     1561 gccgaatggg gaggtcagaa cggcggccac cacggtcagc ccgactagaa gcaccatcac
     1621 ggaccgcacg gctggtccga cctgctgtcc cccctccaac gcttcgccga tgtagccagc
     1681 atcgaacctc aacagcgccg cgaccaggtc gagcaggtag aacacgttac cgaaactctg
     1741 gaggggcaac gctatcacca gcgcggttag taccgccgtt accataagga cccggtatgg
     1801 cggggaaggc aacgaccgta ggaagagctc gacggccctg cgggccttcc agttggattc
     1861 ggggtcgtcg gcgagcgcgg cggcagcatg gtaacggcgg ttgagggcgt tcaggtttct
     1921 gttgatgtga tgcgtgacga agtaggtgag gaaccatcgc agatacggaa ccttgacctg
     1981 gcttcccggc cccttgcggg cccatggcag ccagaccacc ccgggaagcg ccccgacctc
     2041 atccagcgtc tcctggtagg cccccagaag ccccaccaga tgctccctgc tatcctggcc
     2101 gacccccggc tcggctgtca taccctccgg aaccgtgcgg ttctcggaag cgggcggcga
     2161 cttctcccgg ccaccggctg ccgcctccgg ctcatggccc gaccagctct tgtccctctc
     2221 caacgtgcct ctcgcctttc gcgctgcaaa cccggaatca gcccgggcgt ggacggcacg
     2281 tattctagct cactcttcga gcggaccggt gcggagtcta gggtcgagtc tcgggcgcgc
     2341 cggttacaac cggtgccgca gccactacgg ggaccccgcg cttccctcgc gggccgcccg
     2401 aagaggaacc gaaaggttcg ctctacaccg ccgtgtagtc gatatcgtag gcccggctca
     2461 acacgatcct cctgacccga tcctccacca ggggggccgc g
//
LOCUS       DBNGVD010000553         1981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2150376, whole genome shotgun sequence.
ACCESSION   DBNGVD010000553 DBNGVD010000000
VERSION     DBNGVD010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2150376"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="AC16CM_14880"
     CDS             <1..996
                     /locus_tag="AC16CM_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3141165.1"
                     /translation="NPAIVVGAGVERAGAFRDVVALAEKLAAPVWQAPISPLAGFPQD
                     HPLFQGHLPPAQRALAEMLSDYDVVLVLGAPVFLYYPYVPGPTVADGTRVLHVSEDPE
                     EAASAAVGESLVGDVGIAVRVLTELVLQTDRLPPPAQEPPAQPEPATPMSVEHVMHTL
                     AGVLPEGVAVFDETASSMAKLHRYVRFNEPGGYHTSAAGGLGFCMPASVGFKLAAPGK
                     PVVCVVGDGSSMYSIQALWSAARYGADVAFIVINNRGYSILKGFRDAIGAGDTVPGLD
                     VEGIGFVDVARGLGVEGELVERNEDLLPAIKRALGAGRPYLLDVVVDPEVPELLK"
     gene            1027..1938
                     /gene="rbsK"
                     /locus_tag="AC16CM_14885"
     CDS             1027..1938
                     /gene="rbsK"
                     /locus_tag="AC16CM_14885"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR3141166.1"
                     /translation="MFVSEVFVVGSINQDFVLSVDRRPEPGETVTEAQLSKGNGGKGA
                     NQAAAAALLGAGVTFLGRVGDDGLGEPLVEALAEKGVDTGLIEASPGWSTGTAFITLT
                     PDGENAITVAPGANRSLTADDVDDASDAIGDARVLVAQMEVPLEVVLRAVEVAARQGT
                     RALVNLAPTREVPRELLQKLDPLVVNEHEAAFLLGKKVEGVEGALSAAPGLLELGPKS
                     AVITLGREGAVFSDGDSTGHVPSPEVEVVDTTGAGDAFVGALAGRLARDASLEDAVAY
                     AARAGAAAVTREGAQGALPTPESVEAL"
BASE COUNT          310 a          657 c          681 g          333 t
ORIGIN      
        1 aaccctgcca tcgtggttgg ggccggcgtg gaaagggccg gcgcgtttcg cgatgtcgtc
       61 gcgctcgccg agaagctcgc tgccccggtc tggcaggcac ctatctcccc gctcgccggt
      121 tttccacagg atcacccgct cttccagggc catctgcccc cggcccagag ggcgctcgcg
      181 gagatgctct cggattacga cgtggtgctc gtcctcggcg cgcccgtgtt cctctactac
      241 ccttacgtgc caggcccgac cgtggcagac ggtacgcggg tgctccacgt ttcggaggat
      301 cccgaggagg ccgccagcgc tgccgtcggc gagagcctcg tcggcgacgt gggcatcgcg
      361 gtcagggttc taaccgagct ggttctgcag acggaccgtc tgccgccgcc ggcccaggag
      421 ccgcccgcgc agcccgagcc agcgaccccg atgtccgtgg agcacgtcat gcacaccctc
      481 gcgggggtgc tcccggaagg tgtcgccgtc ttcgacgaga cggcctcgag catggcgaag
      541 ctgcacaggt acgtcaggtt caacgagccg ggcggctacc acacgtcggc tgcgggcggc
      601 cttgggttct gcatgcccgc ttcggtcggg ttcaagctcg cggcgcccgg aaaacccgtg
      661 gtttgcgtcg ttggtgacgg gtccagcatg tactccatcc aggcgctctg gagcgccgcg
      721 cgctacgggg ccgacgtggc ttttatcgtg atcaacaacc gcggttactc cattctcaag
      781 ggcttccgcg acgctatcgg ggcgggggat acggtgcctg gcctcgacgt ggaggggata
      841 ggcttcgtcg atgtcgcgcg gggtctgggc gtggaaggtg aactggtcga acgaaacgaa
      901 gatctcctgc ccgccatcaa gcgcgccctt ggcgccggaa gaccttacct gctagatgtg
      961 gtggtcgacc ccgaggtccc cgagctattg aagtagaggt cgcccgcaac ccgtagggag
     1021 aggagtatct tcgtgtctga ggtcttcgtc gttggttcca taaaccagga tttcgtgttg
     1081 agcgtggatc gccgtccgga gcccggagag accgtgacgg aggcgcaact ctccaaggga
     1141 aacggcggca agggcgccaa ccaggccgcc gcggcagccc tcctaggcgc gggcgtaacg
     1201 tttctgggcc gcgttgggga cgacggtctc ggagagcccc tcgtcgaggc cctggccgaa
     1261 aagggtgtgg ataccggcct gatcgaagcg tccccaggct ggtctacggg caccgcgttc
     1321 ataaccctca cgcccgacgg ggagaacgcc atcaccgtcg cccccggtgc caaccgcagc
     1381 ctcaccgcgg atgacgtgga cgacgcctcc gacgccatcg gagatgcgcg cgtgctggtg
     1441 gcccagatgg aggtcccgct cgaggtcgtc cttcgggctg tagaggtcgc cgcgcgacag
     1501 ggaacccgcg ccctcgtaaa cctggcccca acgagagagg tccccaggga gctgctgcag
     1561 aagctagatc ccctggtggt caacgagcac gaagccgcct tcctgctcgg caaaaaggtc
     1621 gagggtgtgg aaggtgccct ctccgccgcc cccgggcttc tcgaactggg gccgaagtct
     1681 gcggtgatca cgctcggacg cgaaggagcc gttttcagcg acggagactc tacgggccac
     1741 gttcctagcc cggaggtcga ggttgtcgat accaccgggg ctggtgacgc cttcgttggc
     1801 gccctggctg gcagactcgc ccgcgacgcc tctctggaag acgccgtcgc ctacgccgcg
     1861 cgcgccgggg ccgcggcggt aaccagggaa ggcgcccagg gcgccctgcc gactccagaa
     1921 tctgtggaag ccttgtagcg gcggcttctg ccgaagatca acccctgcgg gttgcgccgg
     1981 c
//
LOCUS       DBNGVD010000554         3169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4701799, whole genome shotgun sequence.
ACCESSION   DBNGVD010000554 DBNGVD010000000
VERSION     DBNGVD010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4701799"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="AC16CM_14890"
     CDS             complement(<1..1904)
                     /locus_tag="AC16CM_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3141167.1"
                     /translation="MEELFGIPTERLMWILLAVFGAAVLILGLSAFRNRVSFRMAARN
                     LPRRGTQTILIVLGLMLATMLFSASFTTGDTLTNSLRVESLKNLGQVDVEVQARGSDS
                     SGPQQFGLSTSERAKYFDAGIAGDVRDRLSDRDRVSGVAPAAIETVPVTATGSDLSEP
                     STDVLGLDQSSMQGFDSLESASGRTLDPSDLGKNEVYLSAEAARGLDVGKGDEVEATF
                     AQKVDPTAVPNAPPEAPSEGFSKAPSDAGAIPETVPVPQPSKLTVAGVYESGANPASS
                     ASMVMPLERLQNRVGEEGRINIVLITHEGPAIDGAAGTDATISALRPLLEENDLKADP
                     VKKDAIEQADSGGETFTSIFLLFGQFSVAAGVLLIFLIFVMLAAERKHELGIARAVGM
                     RRGHLMRMFAFEGALYALLASALGSVAGVGVGWLMVRVIGEAFAGSGFTISFATSPQN
                     VVIAFCLGMVLTFAVVLISSWRVSRLNVVRAIRDIPEPDKKGRSVRGVLLAIATPLAG
                     AGLLWQGLRVDQTGLFMLGISLVIVGVALLARVLRVPERISFTVAGILLLGLWLLPFD
                     FTPSGMTEGIDLFFISGVMIVLAGVWVIIYNSDLLLGAVVAMFGWLRGMPPVLRAAVS
                     YPMQARFRTG"
     gene            complement(1906..2619)
                     /locus_tag="AC16CM_14895"
     CDS             complement(1906..2619)
                     /locus_tag="AC16CM_14895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3141168.1"
                     /translation="MEETTKELMIEAVGVQKVYDAGGLRVRALRGVDLAVGRGEMVAI
                     MGPSGCGKTTLLNVLSGLDDLSDGEVYVDGESIKGMSDRRRTRFRAEKMGFIFQSYNL
                     IPVLSAIENVELPLLVAGHKPKKARRWAEDAVEMVGLADQAKKRPAEMSGGQQQRITV
                     ARSLVNNPAIVWADEPTGALDSETSGEIMDLLVRLNKSEQQTFVLVTHDAAVAARAHR
                     TIRMRDGRITSDEQNHGET"
     gene            complement(2882..>3169)
                     /locus_tag="AC16CM_14900"
     CDS             complement(2882..>3169)
                     /locus_tag="AC16CM_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141169.1"
                     /translation="GAEASFWLLSGAFLVLRYWAGLDRLSLVFLALIVADNLVILGLG
                     VLDYLYTGVFATYQFAIVAVLLYGITFGKRDFKRLDAYLKHKVPRRKPRAR"
BASE COUNT          599 a         1030 c         1016 g          524 t
ORIGIN      
        1 atcccggtac ggaagcgggc ctgcatcggg taggacacgg cggcccgcag gacgggcggc
       61 atcccgcgca gccagccgaa catggcgacc acggcgccga gcaacaggtc ggagttatag
      121 attatgaccc acacgccggc gagcacgatc atgacgccgg agataaagaa gaggtctatg
      181 ccctccgtca taccgcttgg cgtgaaatcg aagggcagga gccacagccc gagcaagagg
      241 attccggcga ccgtgaagga gatgcgttcc gggacccgca gcacgcgggc caggagcgca
      301 accccgacga tgacgagcga gatgcccagc atgaagaggc ccgtctggtc tacccggagg
      361 ccctgccaga ggaggccggc accggcgagc ggggtcgcga tcgcgagcag cacacccctc
      421 acgctgcggc ccttcttgtc cggttccggg atgtcgcgga tggccctcac cacgttcagc
      481 cggctcacgc gccaggagga gatcaggaca acagcgaacg tgagaaccat gccgaggcag
      541 aacgctatca ccacgttctg ggggctcgtg gcgaaggata tggtaaagcc ggagccggcg
      601 aacgcctcgc cgatcacgcg caccatgagc cacccgaccc cgacgccggc gacgctgccg
      661 agcgcgctgg cgagcagcgc gtagagggca ccctcgaagg cgaacatgcg catgagatgc
      721 ccccgcctca tcccgacggc ccttgctata cccagctcgt gcttgcgctc cgctgcgagc
      781 atgacgaaga taaggaagat caggagcacc ccggcggcga ccgagaactg cccgaagagc
      841 aagaagatgc tcgtgaaggt ctcccctccg gaatcggcct gctctatggc gtccttcttg
      901 acgggatctg ccttgagatc gttctcctcg agcagcggcc ggagcgcgct tatggtggcg
      961 tccgttcccg cggcgccgtc gatggccgga ccctcgtggg tgatcaggac gatgttgatc
     1021 cttccttcct cccccaccct gttctgcaac ctctccagcg gcatgaccat cgaggccgag
     1081 gaggccgggt tggccccgct ctcgtacacg ccggccacgg tcaacttcga aggctgcggt
     1141 acgggcacgg tttccgggat ggctccggcg tccgagggag ctttcgagaa gccttccgaa
     1201 ggtgcctcag gcggcgcgtt ggggaccgcg gtcggatcaa ccttctgggc gaaggtggct
     1261 tcgacctcgt cgcccttgcc gacgtcgagg cccctggcgg cttcggcgct caggtagacc
     1321 tcgttcttgc cgaggtccga ggggtctagc gtcctgccgg atgcgctctc gaggctgtcg
     1381 aaaccctgca tggagctctg gtcgaggcca agcacgtcgg tgcttggctc gcttaggtcg
     1441 ctgcccgtgg ccgttacggg cacggtctct atggccgccg gggcgacgcc cgaaacgcgg
     1501 tctctgtcgg agagccggtc gcgaacgtcg ccagcgatcc cggcatcgaa gtactttgct
     1561 cgttccgagg tggacaggcc aaactgctgc ggacccgagg agtcagagcc tctggcctgg
     1621 acctcgacgt cgacctggcc gaggttcttc agagactcga cacggaggga gttcgtcagg
     1681 gtatcgccgg tggtgaagga ggcggagaaa agcatcgtgg cgagcatcag gccgagcacg
     1741 atcaggatgg tctgggtgcc acgacggggc aggttacggg ctgccatccg gaaggagacg
     1801 cggttgcgga aggcggagag gcccaggatc aggaccgccg ccccaaagac ggccaggagg
     1861 atccacatga gcctctcggt cgggatgccg aagagctctt ccatctcagg tctccccgtg
     1921 gttctgctcg tcgctggtga tgcgcccgtc gcgcatgcgg atggtgcggt gcgcccttgc
     1981 cgcgaccgcg gcgtcgtgcg ttaccagaac gaaggtctgc tgctcgctct tgttgagcct
     2041 gaccaggaga tccattatct cgccggacgt ctcggaatcg agagcgccgg tcggctcgtc
     2101 ggcccatacg atggctgggt tgtttaccag cgagcgggcc accgtaatac gttgctgctg
     2161 gccgccgctc atctcggccg ggcgcttttt cgcctgatcc gcgagcccca ccatctctac
     2221 ggcatcctcg gcccaccgcc tggccttctt gggtttgtgg ccggccacca gcaggggtag
     2281 ctcgacgttc tcgatcgccg acagtacggg tatgaggttg taagactgaa agatgaagcc
     2341 catcttctcg gcacggaaac gggtgcgtct tctatctgac atgcccttga tcgactcgcc
     2401 atcgacgtag acctcgccat cggagagatc gtccaggccg gagagcacgt tcaggagggt
     2461 ggtcttaccg cagcccgaag gccccatgat ggcgaccatc tcgccgcgcc cgaccgcgag
     2521 gtcgacgccc cgcaacgcac gcacccgcag gcccccggcg tcgtagactt tctggacgcc
     2581 aacggcctcg atcatgagtt ccttcgtcgt ctcctccaaa aggttccctc ctcgctcctc
     2641 cgaaacagcc cctcgcccac cgaccacgtc tacctccaac accgactaca cactacgcag
     2701 gaacgataag ccggttgctc gggacgtaca tcgggcccgg ggctgaagtt cgctaggtcc
     2761 acgggcggat cgccccacag gtgacgaccc cgcgaatgat acaacgcaaa atcctcggtg
     2821 ccgctggtta gattcacgta accacccgga aaggcgtaag gatctactct gagagactag
     2881 cctaccgggc ccgtggcttc cgccgtggaa ctttgtgttt caggtaggca tcaaggcgct
     2941 tgaagtcccg cttgccgaag gtgatgccat agagcagcac ggcgactatg gcgaactgat
     3001 acgtcgcgaa cacgcccgtg tacaggtaat ccaggacacc cagaccgagg ataacgaggt
     3061 tgtcagcgac gatcagggcc agaaacacga ggctaagcct gtcgagccca gcccagtacc
     3121 tgagcacgag gaaagcgccc gaaagcaacc agaacgaggc ctccgcccc
//
LOCUS       DBNGVD010000555         7047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3062448, whole genome shotgun sequence.
ACCESSION   DBNGVD010000555 DBNGVD010000000
VERSION     DBNGVD010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3062448"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CM_14905"
     CDS             complement(<1..230)
                     /locus_tag="AC16CM_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HIR3141170.1"
                     /translation="MPVAVVDEVGYERLDGETASHLFSAAFSEMGLDLARIGEVSVAL
                     VDATTIHDLNLRFRNRDEPTDVLSFGVDGPYG"
     gene            complement(257..1843)
                     /locus_tag="AC16CM_14910"
     CDS             complement(257..1843)
                     /locus_tag="AC16CM_14910"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR3141171.1"
                     /translation="MKLGKKSPTKFKRKRVDELHRSPTRFERFRAWIGRIPKVRLYVG
                     LTTVTWLALTALMGIGPRPLIERVIAQGIIDPRDRARLESLDLLHPSDPWMALVGVGI
                     VVAAEMWISWYFLERFGERILKTNSAIRLVLAASLTVLFTALARLFITLDFDPYLTPL
                     AGLSIIGTMLLGPRLMFLMVVITSVNVGILSGNNFLLTAALLLGSGFAIYTTVRVDSR
                     QRLLKAGLVIAAVMGVVTFAVGLIGGGGIQESLWLGVLGLGNGLLSLALATVLLPFLE
                     DAFNILTPMKLLELSDPRHPLLDKLLRNAPGTFSHSMQVGLLAERAAERVGANALLAQ
                     VGAYYHDIGKMEHPAYFVETQIAQLNPHADLSPTLSAKVIKRHVKDGLEIGEAWGLPQ
                     EILDIIAQHHGSTRIEYFYRKALEDAPDENSVRESDFRYSSGLPKSREAGIMMLADSI
                     EATVKSLPKPTPKRIEDVVTDTVRRKLEDGQFDECRLTMHDIHEAGEAIRESMIGFLG
                     PRIEYPEPSKESRSGKKAAK"
     gene            complement(1868..2713)
                     /locus_tag="AC16CM_14915"
     CDS             complement(1868..2713)
                     /locus_tag="AC16CM_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3141172.1"
                     /translation="MVRGNEIVLRGTEAAVERAEAVFDGLVGLAEDGHHLTPETAERL
                     YKMADGGGGREVLGDVILTHRGRRVAPKTRNQKAYTDAIRQHQVVFGIGPAGTGKTYL
                     AVALAVEALNRGEVSRIILTRPAVEAGESLGFLPGDVMAKVDPYFRPLYDALYEMVDP
                     ARFQAHLERGIIEIAPLAYMRGRTLNDAFIILDEAQNTTPPQMKMFLTRLGFGSKMVI
                     TGDLTQVDLPKVRVSGLQDARNTLTGVEGVSFVGLKRDDIVRSDLVMRIVDAYEGAGE
                     DGTTQ"
     gene            complement(2946..3389)
                     /locus_tag="AC16CM_14920"
     CDS             complement(2946..3389)
                     /locus_tag="AC16CM_14920"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3141173.1"
                     /translation="MSVRGRIKDDTRTAMKGRDRTRVGALRMLDAALQNAEIEAGRPL
                     TEQEEQTILRRQLKQRDESAEAFRKAGREEQAEAESAEAEIVRAYLPQPLSPAELEQI
                     VETAISESGATSMRDMGNVMGRAMELSEGRAEGRQLSALVRERLQ"
     gene            complement(3386..3730)
                     /locus_tag="AC16CM_14925"
     CDS             complement(3386..3730)
                     /locus_tag="AC16CM_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR3141174.1"
                     /translation="MSEADCIFCKIARGELDAEVVHEEDDVLAFRDINSQAPVHILVI
                     PRQHAANLEEIGELPDRVVKRLFEVASSVAADAGVTEGGYAVRVNNGRDAGQEVFHLH
                     LHVMGGRKLGMP"
     gene            complement(3744..4403)
                     /locus_tag="AC16CM_14930"
     CDS             complement(3744..4403)
                     /locus_tag="AC16CM_14930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR3141175.1"
                     /translation="MLELPAGEAHHLLKVLRAASGDRIEVADRSGRLFTAELRGGRDA
                     AVLEELAAPDLADTEVSLYQAVPKGGRMDLVVEKATEVGVSSIVPLLTERGVVNPREG
                     KVGRWRRVAKAAARQSLRTGVPEVADPVPFSEAVSRVGDRGVLLHNEPGLPPVETVIG
                     SSIALFVGPEGGWGEGELRLAREVGMPLGSLGSYRLRSETAGIVAVARAFATLENRIK
                     H"
     gene            complement(4469..5611)
                     /gene="dnaJ"
                     /locus_tag="AC16CM_14935"
     CDS             complement(4469..5611)
                     /gene="dnaJ"
                     /locus_tag="AC16CM_14935"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3141176.1"
                     /translation="MATKRDYYEILGVSREASEAEIKKSYRRLARSHHPDANPDDNGA
                     EDRFKELTEAYEVLSNPEARRAYDTYGHQVPRGGGGAGGYPGGGEPFGGFEDIFEAFF
                     GDRFGGGGSFFGSSAPQGPSRGGDAEVQVEVSLREAAFGADRDVSVQVVSNCGVCDGV
                     GGTESHTCPTCNGVGSVRTIRESILGQMVTTGRCSTCGGRGRVIDVVCDNCRGSGRVS
                     EIKTHHLRVPEGIESGMRLRVSGAGHAGEPGAPPGDLYVRVKVAEDPELLRDGDDLIH
                     HMRVSFVDATLGAEVNVPTLEGPRTVRLDAGTQPGATLTLGGDGMPRLRRRGRGDIKV
                     VVDVMIPTRLSSEQRELLERFESVSGEETYNGGGGSFFDRLKGVFR"
     gene            complement(5612..6640)
                     /gene="hrcA"
                     /locus_tag="AC16CM_14940"
     CDS             complement(5612..6640)
                     /gene="hrcA"
                     /locus_tag="AC16CM_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3141177.1"
                     /translation="MTNFELSQRQRELLLKTLEIYISTGAPVGSHALSDEVDTSSSTI
                     RAELAQLESLGLLTHPHTSAGRIPTDRGYRYYVDALMEGAPRDETAFAALEDYGNVDE
                     LLHGVSEGMSDVTRLLAVVAGPSVLGESLSRVDYLPLGDGRLLLVVSMQSGASSSASV
                     SLPIPVEDGELREMFDALNSWLGGRPLGTDLELGLAARAALSDHDPRTVAAVLEAVGA
                     LGRATERGVFIQGASALLSRLDDLDPGSLAAVIEVFERRRWLLRLMGDALRRSVSSSG
                     VVVSIGSESGFMNLTGTSLVASAYSRREQPYGVVSLIGPKRMDYDVAITTVRSAAEAL
                     TNCLDARF"
BASE COUNT         1170 a         2418 c         2184 g         1275 t
ORIGIN      
        1 tccccgtagg gaccgtccac cccgaagctg agcacgtcgg tcggctcgtc cctgttcctg
       61 aacctgaggt tgaggtcatg gatggtagta gcgtccacga gcgccactga gacttcgccg
      121 atccgagcga ggtccaggcc catctccgag aaggcggcgg aaaacagatg ggaggccgtc
      181 tccccgtcta gccgttcgta gcccacctca tctaccacgg cgacgggcat ggattgacct
      241 cccttcggtc ggcgggctac tttgccgcct tctttccgct ccgggattct ttcgagggtt
      301 cgggatattc gatacgcggc ccgaggaacc cgatcatgga ctcacggatc gcctcccccg
      361 cctcgtggat gtcgtgcatg gtcaggcggc actcatcgaa ctggccgtcc tccagcttcc
      421 tgcgcacggt gtcggtgacc acgtcctcga tgcgcttcgg ggtcggcttc ggcagggact
      481 ttaccgtcgc ctcaatggag tcggcgagca tcattatgcc ggcctccctg gacttcggca
      541 ggccgctaga gtatcggaag tccgactctc ggaccgagtt ctcgtcaggg gcgtcttcta
      601 gtgccttgcg gtagaagtac tcgatgcggg tcgagccgtg atgctgggct atgatgtcca
      661 gtatctcctg cggcaggccc caggcctcgc ctatctcgag gccgtccttc acgtggcgtt
      721 ttatcacctt ggcggagagc gtcggggaga ggtcggcgtg cgggttaagc tgggcgatct
      781 gggtctccac gaagtatgcc gggtgctcca tcttgccgat gtcgtggtag taggccccga
      841 cctgggcgag cagcgcgttg gcaccgaccc tctcggcggc cctctcggca agcaggccga
      901 cctgcatgga gtgggagaag gtgccaggtg cgttgcgcag cagcttgtcg agaagcggat
      961 gtcgcgggtc agagagctcc agcagcttca taggcgtcag gatgttgaag gcgtcttcca
     1021 gaaacggcag cagcaccgtg gcgagcgcca gcgagaggag gccgttgccc aggccgagga
     1081 cgccaagcca cagcgactcc tggatacccc cgccgccgat caggccaacc gcgaacgtga
     1141 ccacgcccat cacggcggcg atcaccagcc cggccttcag gagacgctgg cgggagtcca
     1201 cgcgcaccgt cgtgtagatg gcgaaccccg aaccaagcag cagagccgcg gtcaggagaa
     1261 agttgttccc gctcaggatg cccacgttga cgctggtgat cacgaccatc aggaacatca
     1321 gcctcggccc cagcagcatg gttccgatga tggagagccc ggccaggggc gtcaggtagg
     1381 ggtcgaagtc cagcgtgata aacaggcggg ccagggcggt aaacaggacc gtgagcgagg
     1441 cggccaggac gagccttatg gccgagttgg tcttcaggat gcgttctccg aagcgctcca
     1501 gaaagtacca ggatatccac atctccgcgg cgaccacgat cccgacgccc acaagcgcca
     1561 tccacgggtc tgaaggatgc aggaggtcca gggactccag cctcgccctg tcgcgggggt
     1621 cgatgatccc ttgagctatc acgcgttcga tgagtggccg cggaccgata cccatcagcg
     1681 ccgtcagggc gagccaggtc acggtggtga gcccgacgta caacctgacc ttgggaatac
     1741 gcccgatcca ggccctgaag cgctcgaacc tggtgggcga gcgatggagc tcgtctacgc
     1801 gtttgcgctt gaactttgtg gggctctttt ttcctagctt catgattgct cccggctcga
     1861 cggccctcta ctgggttgtg ccatcctccc cagcaccttc gtaagcgtcc actatgcgca
     1921 taacgaggtc actcctgacg atgtcgtcac gcttgagccc aacaaatgat accccttcca
     1981 cgccggtcag ggtgtttctg gcatcctgca gcccgcttac gcgaactttc gggaggtcta
     2041 cctgcgtgag gtcgccggtg atgaccatct tggagccgaa gccgaggcgc gtcaggaaca
     2101 tcttcatctg cggcggggta gtattctggg cctcatccag gatgatgaac gcatcgttca
     2161 aagtgcggcc ccgcatataa gcgaggggcg cgatctcgat tataccgcgc tccaggtgcg
     2221 cctggaacct ggccgggtcc accatctcgt agagcgcatc gtagagcggg cggaagtagg
     2281 ggtctacctt ggccatcacg tcaccgggaa ggaaccctag cgactctccg gcctccacgg
     2341 cgggcctggt caggatgatc ctcgaaacct cacccctgtt gagggcctcc acggcgagcg
     2401 ccacagccag gtaggtcttg cccgtgcccg cgggcccgat gccaaagacg acctgatgct
     2461 ggcggatggc gtcagtatag gccttctggt tccgggtctt gggcgccacg cgccgcccgc
     2521 gatgggtcag gatcacgtcg ccaagaacct cgcgcccacc gccgccgtcg gccatcttgt
     2581 agaggcgctc ggcggtctca ggggtaagat ggtggccatc ctcggcgaga ccgaccaggc
     2641 cgtcgaagac ggcctcggcc ctctccacgg ccgcctcggt gccccggagc acgatctcgt
     2701 tgccccgcac cacgacctca cactctacga gttcctcgac cctccgcaga acagagtccc
     2761 tgtccccgaa gacttccagc gttcgctgcg gcggtattac gagcttgacc tctacctgac
     2821 tatcggatct ggtttccgtg tttactcctt cgtcgtagct ttcagctgtc agccttcagc
     2881 tatcagcttt ctggtggttg gtgtggcttt cggcgcacaa cggttccaac tgatgcctga
     2941 agccttcatt gcaacctttc tctcaccaaa gcggagagtt ggcggccttc ggcgcggcct
     3001 tcggagagtt ccatggcgcg gcccattacg tttcccatgt ccctcatgct cgttgctccg
     3061 gactcgctta tggccgtttc gactatctgc tccagctccg caggcgagag gggttggggg
     3121 aggtaggcac ggacgatctc ggcctcggcc gattctgctt cggcctgctc ctcgcggccg
     3181 gctttgcgaa acgcctcggc tgactcgtcg cgctgtttta gctggcgcct gaggatggtc
     3241 tgctcctcct gctcggtgag cggccttcct gcctctatct cggcgttctg gagcgcggcg
     3301 tccagcatcc tcaaagcgcc gaccctggtc ctgtcgcgcc ccttcattgc ggtccttgtg
     3361 tcgtccttga tcctgcccct gacactcacg gcatccccag cttcctgccg cccatgacgt
     3421 gcaggtggag gtggaagacc tcctgcccgg catcgcgccc gttgttgacc ctgacggcgt
     3481 agccaccctc cgtgacgccg gcgtccgcgg ccacggagct cgccacctcg aaaagacgct
     3541 tgaccacccg gtcgggcagc tccccgatct cttcgaggtt ggcggcgtgt tgcctgggga
     3601 tgacgaggat gtgcacgggg gcctggctgt tgatgtcgcg gaacgccagc acgtcatcct
     3661 cctcgtggac cacctcggcg tccaactccc cccgcgctat cttacagaat atgcaatcag
     3721 cctcgctcac atccacttct cctctagtgt ttgatcctat tctcaagggt ggcaaacgcc
     3781 cgcgccaccg ccacgatgcc cgccgtctcg ctgcgcagcc tgtacgaccc caggctaccc
     3841 aacggcatgc caacctcacg tgcgagccgt agctcaccct cgccccaccc tccttctggt
     3901 cccacaaaga gggcaatgga tgacccgatg acggtctcga cgggaggaag acctggctcg
     3961 ttgtgaagga gcacgccccg atccccaacc cgcgagacgg cctccgagaa cggcaccggg
     4021 tccgccacct caggaacacc cgtccggagc gactgccgcg ccgcggcctt ggcgaccctt
     4081 cgccagcggc ccacctttcc ctcgcgcggg ttcacgacgc cacgctccgt cagcaacgga
     4141 actatgctcg agaccccgac ctccgttgct ttctccacca cgaggtccat cctgccgcct
     4201 ttgggaaccg cctgatacag cgagacctcc gtgtcggcca aatcaggcgc cgcaagctct
     4261 tccaggacgg ccgcatctcg ccccccgcgc agctcggccg tgaagagacg cccggagcgg
     4321 tcggcgacct cgatacgatc cccgcttgcc gcacgcagga ccttgaggag gtgatgggcc
     4381 tcaccagccg gaagttcgag catcccgccg ggccccggaa cggagtcgac gaagattcgg
     4441 gttatggtag tgccagcgac gaagccccct acctgaaaac gcccttcaga cggtcgaaga
     4501 aggaaccccc gccgccgttg taagtctctt cgccgctgac gctctcgaac cgctccagaa
     4561 gctcccgctg ctccgaggag aggcgggtcg ggatcatgac gtcaacgacg accttgatgt
     4621 ctccgcgccc gcgtcgtctg agccggggca tcccgtcgcc cccgagcgtg agcgtggcac
     4681 ccggttgcgt gccagcgtct agcctcaccg tcctcggccc ttccagggtc ggcacgttca
     4741 cctcggcccc tagcgtggcg tccacgaagc tgacccgcat gtggtggatg aggtcgtcgc
     4801 catcccgcaa caactcgggg tcttccgcga ccttgaccct cacatacagg tctccaggcg
     4861 gggcgcccgg ttcgccggcg tgcccggcgc cggagacgcg caggcgcatg ccgctctcta
     4921 tgccctcggg aacccgcagg tggtgggtct tgatctcgct taccctcccg ctgccgcggc
     4981 agttgtcgca cacgacgtcg atgacgcgtc cgcgcccccc gcacgtagag cagcggcccg
     5041 tggtaaccat ctgaccgagg atgctctccc ggatcgttct tacggacccg acgccgttgc
     5101 aggtgggaca ggtgtgagac tccgtgcccc ccaccccgtc gcagaccccg cagttgctta
     5161 ccacctggac gctcacgtcg cggtcagccc cgaaagcggc ctcccgcagc gaaacctcga
     5221 cctggacctc ggcgtcgcca ccgcgggacg gcccctgggg cgccgagctt ccgaagaacg
     5281 agccgccacc gccgaagcgg tcgccgaaga aggcctcgaa gatgtcctcg aaaccgccga
     5341 agggctctcc cccacccggg tagccgccgg cgccaccgcc gccgcgtggg acctggtggc
     5401 cgtatgtatc gtaggcgcgc cgtgcctcgg ggttggagag gacctcgtag gcttcggtca
     5461 gctccttgaa gcggtcctcc gcgccgttgt catccgggtt ggcgtcgggg tggtgggagc
     5521 gggccaggcg gcggtaggac ttcttgatct ccgcctccga cgcctcccgg gacacgccca
     5581 gaatctcgta gtagtctcgc ttggtggcca actagaaccg ggcgtccagg cagttggtca
     5641 gggcctcggc ggccgagcgg accgtcgtta tggccacatc gtaatccata cgcttcgggc
     5701 cgatcaggct aaccaccccg tagggctgct cgcgacggct gtaggcggag gccaccaggc
     5761 tcgtgccggt caggttcatg aagccgctct cgctgccgat cgagacgacc accccgctgc
     5821 tcgagacgga gcgcctcagg gcgtcgccca tcaaccgcag gagccaccgg cggcgctcga
     5881 acacctcgat gacggcggcc aggctgccgg ggtcgaggtc gtccaggcgg ctgagcaggg
     5941 ctgatgcacc ctggataaaa acgcccctct ccgtcgcccg tcccagggcc ccgacggcct
     6001 ccagaacggc ggccacggtc ctcggatcgt ggtccgagag ggcagcgcgc gccgcgagcc
     6061 ccagctccag gtcggtccca aggggccgtc ccccgagcca ggagttgagc gcgtcgaaca
     6121 tctcccgcag ctctccgtcc tctacgggga tgggtaacga aaccgaggcg ctggaggaag
     6181 ccccactctg catggagacc acgagcagga gtcggccatc gcccagcggc agatagtcca
     6241 cgcgagagag cgactcgccc agcacgctcg gtcccgcgac caccgccagc agacgggtaa
     6301 cgtcgctcat cccctccgag acgccgtgca gcagctcgtc cacgttgccg taatcctcca
     6361 gggcggcgaa cgccgtctcg tccctggggg caccctccat cagggcgtcg acgtagtacc
     6421 tgtaacccct gtccgtcggt atgcgcccag cggaggtgtg cgggtgggtc agcagcccga
     6481 gcgactccag ttgggccagc tcggcccgaa tcgtggagct gctagtgtct acctcgtcgg
     6541 atagggcatg gctacccacg ggcgcacccg tggagatata gatctccaac gtcttcagca
     6601 gcaattcacg ctgcctctgt gatagctcga agttcgtcat cttgcgcgtg attttaacat
     6661 gagcacgctg cgccctacgg tgagcacgct ccggctaacg cctccgcgtg cgcacgcccg
     6721 cttcgctccg ctacgccggc tcctcagcac gctcgggctc gcgccctcgc ttgtcagcaa
     6781 cgcatcggct tcgcctcgct ttcggccggt cgtctcgatt tgcatcggtg gcttcgacat
     6841 aaggctttcc cgggcatcga atctctagag cggaaggtgc tggccgcagc gggacctggc
     6901 tgacagcgag ctctaggcgc agcctggcga agctttgctg acaagcgaca gccgccaggc
     6961 tggagcgtgc tgacaaacgg aggcccgcag ggccggagtg tgctgaaagc gtaggcgtta
     7021 gccgaagctt gctagatacg tagtgcg
//
LOCUS       DBNGVD010000556         5755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1240065, whole genome shotgun sequence.
ACCESSION   DBNGVD010000556 DBNGVD010000000
VERSION     DBNGVD010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1240065"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC16CM_14945"
     CDS             complement(<1..331)
                     /locus_tag="AC16CM_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3141178.1"
                     /translation="MTAPASVTITPIRVADLLAEGEPMPLYVHVIDHPDARVLVDTGM
                     TEEHPAVRAALDPHLYPLSEQDLDLAGVDIVVNTHLHADHCGGNHLFAGKPIYVQRRE
                     LDDALSEE"
     gene            877..1224
                     /locus_tag="AC16CM_14950"
     CDS             877..1224
                     /locus_tag="AC16CM_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3141179.1"
                     /translation="MFTAYVVVTLVAITASAFVATANFMRLDFVLATADRVRVPRSWM
                     PSLGLLNAAAAIGLLVGLIGVQPVGTAAALGLVLYFVGAIITHLRARDYSLGPPAAFL
                     LLAVAALVLGLAS"
     gene            complement(1464..2783)
                     /locus_tag="AC16CM_14955"
     CDS             complement(1464..2783)
                     /locus_tag="AC16CM_14955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3141180.1"
                     /translation="MEPKPAQTEVAVVGGGISGLTAACFLAREGARVTLFEKASAPGG
                     RAATRESGAFLFNRGIHALYTGGAASRAFEELGVSYGHGTPKETFVLDGGDLRPFPTS
                     PSQFLGTDLLGAGDKLALVRFFAGLARARPKDLAGKSLQGWLDEKVRRPRLRRLLRSL
                     AYPLVYTSALDLVSAEVFVDKFQRALKHPVHYVDGGWRVLVDGLREVAERAGTRVVTG
                     ARVESVEVGNGRASGVRLRDGSVVRAGAVVLATGPRDASKLVGGGELGRTVDGLIPAR
                     VACLDVALERLPSPDVPVVQDLGGPRFMTAQSVYSRVAPEGAALIYTFKQLDPRRSGD
                     PREDEWDLEALLDAAQPGWRDVIVDRQYLPRIEAVGALPTAEGGGFAGRPGPRVPGIA
                     GLYLAGDWVGPEGFLVDASVASARSVARLALEDDPSATREVATGSVR"
     gene            3095..3415
                     /locus_tag="AC16CM_14960"
     CDS             3095..3415
                     /locus_tag="AC16CM_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141181.1"
                     /translation="MTEAGGGDGRAEVTRRLIERSLEDEEFRRGLLETPRSAVEQELG
                     TQLPEGVEVRVVEETADTIYLVLPSSSPGDQSGELSEQELEVVAGGSSSQSGMCSICG
                     WVSQ"
     gene            complement(3510..5087)
                     /locus_tag="AC16CM_14965"
     CDS             complement(3510..5087)
                     /locus_tag="AC16CM_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3141182.1"
                     /translation="MSDERVIAVDLASVYETAEKDGLIRILAWGDRVEVEEVTDEHVR
                     IETTRFETTPDGSIEPVPASGFIVPSAASGIEPHEVVATREEAGVLKVDFVDVQQGDA
                     SAIEMPDGRIVLVDGGDNQLFARYLASRYRNTSEEEPRDVECIVVTHGDADHFAGLVE
                     IQESETHPLEYKRFFMRPRRVYHNGLIKRPSSVAEKDSFGQSVEVGEKRIITELETDL
                     LGVDDSSMNQYFQRWKEALSAYDGREGGTIEFRRLAIGDDDAFDFLGEENLKVEVLGP
                     IPTREDGVEGLRFLGEPEDGPRVGHHSLGGSSGSRFSGLSASHTINGHSIVLRLTCGS
                     FSFLFAGDLNEEAETDLLRAHEQGEVDLRSEVLKTPHHGSDDFLPEFIGAVAPIISVV
                     SSGDENARKEYIHPRATLVGALGKHSRAEEPIIFITELVAFFQAEGFMRPEFHELAGD
                     GTPLIEHDRPVTDREAEGRGSFFAFSRAAFGLVMVRTDGDRLLVYTNSARTTFKEVYA
                     FGTDGAGEPVPVKVRRV"
     gene            complement(5198..>5755)
                     /locus_tag="AC16CM_14970"
     CDS             complement(5198..>5755)
                     /locus_tag="AC16CM_14970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974596.1"
                     /note="catalyzes the formation of 3-methyl-2-oxobutanoate
                     from 2,3,-dihydroxy-3-methylbutanoate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3141183.1"
                     /translation="CVIKHSAADPGLLRHTGPALVFDSYDDLERRVNDPGLDVDENSV
                     LVLRGAGPLGAPGMPEWGMLPIPEKLLKKGVRDMVRISDARMSGTAYGTCILHVAPEA
                     HAGGTLALVRDGDLIELDVPNRRLEVRVGEQELGRRRAAWKAPAPRYERSYGALYASH
                     VTQANEGCDFDFLAEGPAPEEPAIH"
BASE COUNT          940 a         1944 c         1885 g          986 t
ORIGIN      
        1 cctcctcgct cagcgcgtcg tcgagctccc gacgctggac gtagatcggc ttgccggcga
       61 agaggtggtt gccgccgcaa tggtcggcgt gcaggtgcgt gttgacgacg atgtcgacgc
      121 cggcgagatc caagtcctgc tcgctcagcg gatagaggtg gggatcgagg gcggcccgga
      181 ctgccggatg ctcctccgtc atgccggtgt cgaccagcac gcgcgcgtcg ggatggtcga
      241 tgacatgcac gtagagcggc atcggctcgc cctcggcgag caggtcggcg acgcggatcg
      301 gcgtgatggt aaccgaggcg ggggcggtca tgacgtaccg cccgccgcgg tggtcgttga
      361 catgtgagcc atgaagatct cctttaccgg tctccggctg gtgcagggcg atgtggcccc
      421 cgcttgccga ttcagccgcc cctataggcg ttgaacactg ctctttataa tggtggaaga
      481 ggcgagatct ccaaacacct actttcgccg gttacccacg aaacgggcga tgctgtctcg
      541 ccttcggatg ctctctgggg acgcctatca tggcaggttc acggcggggt cggggcggca
      601 gagacgcagc caggcctcct ggaaggcatc gtccgcctca ccggccgtga ggagcattct
      661 gtcccccagg cgacccccgt gctactcgaa ccgactgcca ggagatccta ttcgtccaca
      721 agtcacgttc ttccgtcgcc ctccgtcacc gtaatagtga agtgccagat caggccagag
      781 tgacgaggag gtgacgagga atccggcttc gcgcggaacg cgggtgaccg gatggcaccc
      841 gaacgaaagc acatcggtaa gggataggag ttcgctttgt ttaccgcgta cgtcgtcgta
      901 accctggtgg cgatcaccgc cagcgccttc gtggccactg ccaacttcat gcgcctcgac
      961 ttcgtcctcg ccaccgcgga cagggtgcgc gtgccacggt cgtggatgcc ctccctgggc
     1021 ctcctcaacg cggccgccgc catcggtctg ctggtcggcc tcatcggcgt gcagccggtc
     1081 gggacggccg ccgccctcgg ccttgtcctg tacttcgtgg gcgctatcat cacccacctg
     1141 cgcgcacgcg actactcact aggcccaccg gccgcgttcc tcctgctggc cgtggccgcg
     1201 ctggtgttgg gactggcctc gtaaatcatc ccggagtgga tccgcactgg ttcccgctcc
     1261 gagccattcg gctcccctgg caaacaccag ggagagcacg atagacacag ccgttacagg
     1321 cgacggatca tcggaacgag ggcgtcgagg ctggtgggga tggtggcgtg tacccgagaa
     1381 ggcctctgcc atcacacgtc acgcaatata ccggagccct ggacgggcgt gcccgtaggc
     1441 gcgtgccttc tgtcaggcag cctctagcgt acggaaccgg tggcaacctc cctcgtcgca
     1501 gacgggtcgt cctcgagagc cagccgcgcg acgctccggg cgcttgccac gctggcgtcc
     1561 accaggaatc cctccggtcc gacccagtct ccggccaggt agaggccggc gatgccgggc
     1621 acccgggggc cgggacgacc cgcgaagccg ccaccctcgg cggtcgggag agcgccaacg
     1681 gcctcgatgc gcggcaggta ctggcggtcg acgatgacgt cgcgccagcc gggctgggcc
     1741 gcatcgagca gggcttcgag gtcccactcg tcctcgcgcg ggtccccaga cctcctgggg
     1801 tcgagctgct tgaaggtgta gatgagggcg gctccttcag gggcaaccct ggagtagacg
     1861 gactgggccg tcatgaagcg gggacccccg aggtcctgga ccacaggaac gtccggcgag
     1921 ggcaggcgct caagggccac gtccaggcag gccacccggg ccgggatcag cccatccacg
     1981 gttcgtccca gctctccccc acccaccagc ttcgaggcgt cgcggggtcc cgtagccaga
     2041 accacggcgc cagcccgtac cacgctcccg tcgcgcagcc tcacgccact cgcccgaccg
     2101 ttgccgacct cgacggactc cacgcgggcg ccggttacca cgcgcgtccc ggcgcgttcg
     2161 gcaacctccc gcagcccgtc gaccaggacc cgccagccgc cgtcgacgta gtgtacggga
     2221 tgcttgaggg cacgctggaa cttgtctacg aagacctccg cgctcaccag gtcgagcgcg
     2281 gaggtgtaga ccaaggggta ggcaagcgag cggagcaacc tcctcaggcg cggacgcctg
     2341 accttctcgt ccaaccaccc ctgcaggctc ttcccggcca aatccttcgg tctagccctg
     2401 gcaagcccgg cgaagaaacg gacgagcgcc agcttgtctc ccgcgccgag taggtctgta
     2461 cccagaaact gcgaggggct cgtcgggaaa ggacgcaggt ccccaccgtc tagcacgaag
     2521 gtctccttcg gggtaccgtg gccgtaggat acgcccagct cctcgaaggc ccgcgaggcc
     2581 gcgccgccgg tgtagagggc gtgtataccg cggttgaaga ggaaggcccc cgactccctg
     2641 gtggcggccc gtcctccggg ggccgaggcc ttctcgaaga gggtcacacg cgcgccttcg
     2701 cgggccagaa agcacgcggc ggtgaggccc gagatcccac ccccgaccac cgccacttcc
     2761 gtctgtgctg gtttcggttc catccccgtg ctctcctttt cctccgcgta cggaggttcg
     2821 acgtacacgc gtcggtgcga ctgcacccag gacgcgctat gagcgtacga gcgtgacata
     2881 tccagaaccc gcgacgtcgc agcggcacgc ggatcaacct ctgttccgcg atgttccggt
     2941 cccgggtcag cggggcgcca gaccggccga ctcacgggta ttcggcttgt gagagcctgt
     3001 agcgcgctgc ttgcccgacc gatggcggtg tgagatattt gccctgcgcc ccagaaggcg
     3061 cgaccatgca aggcggcaag aaaggggaag gatcatgacc gaagcgggcg gtggagacgg
     3121 acgcgccgag gtgacgcgca ggctcatcga gaggagcctc gaagacgagg agttccgccg
     3181 ggggttgctc gagaccccga ggtcagccgt ggagcaggag ctcgggacac agctgcccga
     3241 gggcgtcgag gtaagggtgg tggaagagac cgcggacacc atctacctgg tgcttcccag
     3301 ctcttcgcca ggcgaccaga gcggcgagct ctcggagcag gaactcgagg tcgtggccgg
     3361 cggctcttcc tctcagtccg ggatgtgttc catatgcgga tgggtctcgc agtaacaccc
     3421 ctactacctc tagattcggc ggggtgggaa gaggttggcc aggctgcccc gtcacacatc
     3481 atccagagat cgcagggcag tcggcaggct catacgcggc gaactttcac ggggacgggc
     3541 tcgccggccc cgtccgtccc gaaggcgtaa acctccttga aagtcgtcct ggcgctgttc
     3601 gtgtacacga gcagacggtc gccgtccgtc ctcaccatta ccagcccgaa agcggccctg
     3661 ctaaacgcga agaagctacc gcgtccctcc gcctctctgt ccgtgacggg ccggtcgtgc
     3721 tctatcaggg gggttccatc ccctgcgagc tcgtgaaact cgggcctcat gaagccttcc
     3781 gcctggaaaa aggcaaccag ctccgtgatg aagatgatcg gctcttccgc ccgcgagtgt
     3841 ttgccaaggg ccccgaccag ggtcgcccgc gggtggatgt actccttgcg ggcgttctcg
     3901 tcgccggacg ataccaccga tattatcggc gctaccgccc cgataaactc gggcaggaag
     3961 tcgtccgagc cgtggtgcgg cgtcttcagc acctcggagc gtaggtcaac ctccccctgc
     4021 tcgtgggcgc gcagcaggtc ggtctccgcc tcctcgttga gatccccggc gaagagaaag
     4081 ctgaagctac cgcaggtcag gcgcagcacg atggagtggc cgtttatggt gtgcgacgcg
     4141 gagaggccgg agaacctgct gccggaagag cccccgagcg agtggtgacc cacgcggggg
     4201 ccatcctccg gctcgccgag aaacctgagg ccctcaaccc catcctcccg ggtcgggatt
     4261 gggccgagca cctccacctt caggttctcc tcgccgagga aatcgaaggc gtcatcgtcc
     4321 cctatggcca atcggcggaa ctcgatggtg cctccctcgc gcccgtcgta ggcggatagc
     4381 gcctccttcc accgctggaa gtactggttc atactcgagt cgtcaacccc gagcaggtcc
     4441 gtctcgagct cggtaatgat ccgcttttca ccgacctcca cgctctgccc gaagctatcc
     4501 ttctccgcca cgctgctggg ccttttgatc aggccgttgt ggtaaacgcg cctggggcgc
     4561 atgaagaagc gcttgtactc gagggggtgg gtctcggact cctggatctc gacgagcccg
     4621 gcgaaatggt cggcatcccc gtgggtcacg acgatgcact cgacgtccct tggctcctcc
     4681 tcgctggtgt tccggtagcg gctcgcgagg tagcgggcga agagctgatt gtcgccgcca
     4741 tccacgagaa cgatccggcc atccggcatc tcgatcgcag aggcatctcc ctgctgcacg
     4801 tcgacgaagt ccaccttcag gaccccggcc tcctcccgcg tggccaccac ctcatggggc
     4861 tctatcccgc tggcggccga cggaacgatg aacccggagg cgggtaccgg ctcgatactt
     4921 ccgtctggcg tcgtctcgaa cctggtggtc tcgatccgga cgtgctcgtc ggttacctcc
     4981 tctacctcta ccctgtcgcc ccaggcgagg atccggatca ggccgtcctt ctccgccgtc
     5041 tcgtagaccg atgcgagatc caccgcgata acccgctcgt cgctcatctg ccacgccccc
     5101 aggtcctgga ctacataacc cgcccgtaca tccgccgtgc gctatggacg tgggattaat
     5161 tctgccatcg gcgggggtag tacggaagcg aatggggcta gtgaatggcc ggttcttcgg
     5221 gggctgggcc ttcggcgagg aagtcgaagt cgcagccctc gttggcctgg gtgacgtggc
     5281 tggcgtacaa cgcgccgtag ctgcgctcgt agcggggtgc tggggctttc caggcggctc
     5341 gccggcgtcc cagctcctgc tcgccgacgc gtacttcgag gcgacggttc gggacatcga
     5401 gctcgatcag gtcgccgtcg cgcaccaggg cgagcgtccc gccggcgtgg gcttcggggg
     5461 cgacgtgcag gatgcaggtg ccgtaggcgg tcccgctcat gcgcgcgtcc gagatgcgga
     5521 ccatgtcgcg cacgcccttc ttcaagagtt tttcggggat cgggagcatt ccccactccg
     5581 gcatgcctgg cgccccgagc ggcccggccc ccctgagcac gagcacggag ttctcgtcga
     5641 cgtcgagccc aggatcgtta acccgccgtt cgaggtcgtc gtagctgtcg aagaccaggg
     5701 cgggaccggt gtgccgcagg agcccggggt cggctgcgga gtgttttatg acgca
//
LOCUS       DBNGVD010000557         4374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_513065, whole genome shotgun sequence.
ACCESSION   DBNGVD010000557 DBNGVD010000000
VERSION     DBNGVD010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_513065"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16CM_14975"
     CDS             complement(<1..434)
                     /locus_tag="AC16CM_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141184.1"
                     /translation="MDTREKKPLPDERVDYFEERGLGPWIKRNRWLLARLFTVVVFSF
                     AASTVATNFSYSQEFRPTVLSVDQLNSGRLPAGTQLGDYVEVSGTPDVGPIKPTTIGR
                     PESRIGVVSRYETAYLYYRLDETGDNLLIQSTQGLPSDIQTSG"
     gene            617..1255
                     /locus_tag="AC16CM_14980"
     CDS             617..1255
                     /locus_tag="AC16CM_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141185.1"
                     /translation="MVEGREIPEQGSWYTVVGVVDDGVGLNQAVTEIRELGVDRDELT
                     VVLKRVNASEPEPFPEGTRYIVIPADRRGLEVPLGFAIAFIVFGIFFAITTPAIGIPT
                     LMVFISLAAILFAASLTRVGVTPILMEMGAPQEEADTWNDAFEQGKVLVFASTGERRL
                     LRPVREAVQRVGGLYYIVDSRLEPRAVHQATLHRSGGGHQAGSSVFERTGEA"
     gene            1278..2612
                     /locus_tag="AC16CM_14985"
     CDS             1278..2612
                     /locus_tag="AC16CM_14985"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3141186.1"
                     /translation="MGVAIISSLHLLAQLNLTDAFSNVGGTRFITGFSMIVHMFFAEL
                     FVGFALAAPVLQAWGARTGSPRMDRLAHSMARFNVLTFSAGATFAVLFVVLLVGLYPR
                     VTTSLFTHFFYFIALAMLSMGLALWGMYSYYYKWDRYSVLKKKRHIALGFTMGFFIWI
                     WMVIMTGIDTYMVTGAPGVKPISDSTIESFGVAVQGIFNPMFTEMVLHRTFANLSWPA
                     FAIAAWASFMYIRSKTSEDRAFFDWSSSVGLTWGVGFLMLQPLVGFTLVYALKLSQPA
                     NLANPSAPGAYDRLVAGESSNLLLINTIIVVTLFVLSNAAMYFGAERHPDRAGRVPIR
                     FFGLVAAVAGLYAISPLAEFPFLYMRYIMLTIMILATLGAYVTYLRGRLRFRYGSPGR
                     SYRVVLIATGVVAAVIALNMGFMKSNSRVPYTVYNQPGYTVEPGPPPTGFDQ"
     gene            2647..3360
                     /locus_tag="AC16CM_14990"
     CDS             2647..3360
                     /locus_tag="AC16CM_14990"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3141187.1"
                     /translation="MVELQKDPLTRSDFLGFGVMGAIMGAILTIPPVAFVLSPIIKTD
                     VLGESDVGSGWVEVASVADVGVEEPSHFRVEFPLQQTYGERAIQDEFPNSGKSRMEYT
                     LANAIWLSWRAPIKQPGRQGSNGDVIGTPERPAILDQKSSGFTESERKEIMSKLNVLS
                     NSCAHLGCPVRWMSDQQLYLCPCHGGLYDINGGWVGGPPPRGMYRYTEAEVRENGKLY
                     VKHAYDIEPGLEKQEPYVV"
     gene            3370..4200
                     /locus_tag="AC16CM_14995"
     CDS             3370..4200
                     /locus_tag="AC16CM_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3141188.1"
                     /translation="MIGRIRNQVTDASQFMVDWMEHRTGIVTITEHFLYEPVPKRGAW
                     LYTLGSAVLFTITLQFLTGILLLFYYVPTTDDAWNSVSYIMNDAYFGQVIRGIHYWSA
                     NFLLAFIGLHMGQVFFSGGYKAPRELNWVVGVILLLLVIGLAFTGYLLRWDQDGFWAS
                     VVGMKIGSYSPFVGGYVINFLLGGDAVGPATLSRFFAIHVWLLPAAIAPLIGAHLYLL
                     RRHGVFGSEFEYTDRLAKLHERQRMEDYARMETGEDDGEPYVGRRDRADREGYERDGE
                     "
BASE COUNT          777 a         1326 c         1409 g          862 t
ORIGIN      
        1 ccggaggtct ggatgtcgct cggcaggccc tgggtcgact ggatgaggag gttatcgcct
       61 gtctcgtcga gacggtagta aaggtaggcg gtctcgtagc gggagacgac ccctatccgg
      121 gattcggggc gcccgatggt ggtcggtttt atcgggccca cgtcgggggt gccgcttacc
      181 tcgacgtagt ctccgagctg cgtgccggcc gggagtcgtc cggagttgag ttggtccacg
      241 ctcaggaccg tgggcctgaa ttcctggctg taggagaagt tggtcgcaac cgtggaggcg
      301 gcaaaggaga agactaccac cgtgaatagg cgcgcgagca accaccggtt gcgtttgatc
      361 cagggcccga gcccccgctc ctcgaagtag tctactcgtt cgtcgggaag tggcttcttc
      421 tctctggtgt ccacgaggtc agtctaccct agcgcgtggt gggcgttgcg cacgccttca
      481 catccggcgg tttgtgaaag agattcccgc gccgccggtg gaacggtcta gtatgcggag
      541 gctagacaat gtatgctgtt ggacggcccg aatcggtgct tgggacaggt caatttcctc
      601 tctggaggta ggcgttttgg tcgaagggcg ggagataccg gagcagggct cctggtacac
      661 cgtagtagga gtagtggacg acggggtggg tctcaaccag gccgttacgg agatccggga
      721 gctcggcgtg gacagggacg agctgacggt ggtcctcaaa cgtgtgaacg ccagcgagcc
      781 cgagccgttc ccggagggca cgcgctatat cgtgatcccg gccgacaggc gcgggctgga
      841 ggtgcccctt ggcttcgcca ttgcgttcat agtcttcggc atcttcttcg ccatcacgac
      901 gccggccatc ggcataccta ccctcatggt cttcatctcg cttgcggcca tactcttcgc
      961 cgcctcgctc acgagggtcg gggtcacccc catccttatg gagatgggcg cgccccagga
     1021 ggaggcagac acctggaacg acgcgttcga gcagggcaag gtgctcgtct ttgcctcgac
     1081 cggcgagcgc aggctgctcc ggcccgtgcg cgaggccgtg cagcgcgtcg gggggctgta
     1141 ttacatagtg gacagcaggt tggagccgag ggccgtgcat caggccaccc tgcacaggtc
     1201 cggcggtgga caccaggctg gcagctcggt gttcgagcga accggcgaag cctagataga
     1261 gaataggagt ttcgagcttg ggcgtagcaa tcatttcgag cctgcacctc ctggcccaat
     1321 tgaacctcac ggacgccttc tcgaacgtgg gcggaactcg cttcataacg ggcttttcca
     1381 tgatcgtgca catgttcttc gccgaattgt tcgtcggctt cgccctcgcg gcgccggtct
     1441 tgcaggcctg gggggcgcgc acgggcagcc cgaggatgga ccggctcgcc cactccatgg
     1501 cccgcttcaa cgtgctcacg ttctccgcgg gagccacctt cgcggtcctg ttcgtggtcc
     1561 tcctcgtcgg cctctacccg cgggtcacga cctcgctgtt cacccacttc ttctacttca
     1621 tcgccctggc catgctctcc atgggcctcg cgctctgggg catgtattct tactactaca
     1681 agtgggatcg ctacagcgta ctcaagaaga agcggcacat tgcgctcggg ttcaccatgg
     1741 gcttctttat ctggatctgg atggtcatca tgaccggcat agacacctac atggtaacgg
     1801 gtgcccccgg ggtcaagccg atctcggact cgaccataga gagcttcggg gtggccgtgc
     1861 agggcatctt caaccccatg ttcacggaga tggtcctgca caggacgttc gccaacctct
     1921 cctggccggc cttcgccatc gccgcgtggg cctcctttat gtacatccgc tccaagacct
     1981 cggaggacag ggccttcttc gactggagca gctccgtcgg cctcacctgg ggcgtggggt
     2041 tcctgatgct gcagccgctc gtgggcttca ccctcgtgta cgcgctcaag ctctcccagc
     2101 cggccaacct ggccaacccg tccgctcccg gcgcctacga caggctcgtc gccggggagt
     2161 cgtcgaattt gctcttgatc aacaccatca tagtcgtgac cctgttcgtg ctctccaacg
     2221 cggccatgta cttcggggcg gagaggcatc ccgaccgcgc gggccgggtg cccataaggt
     2281 tcttcggcct cgttgcggcg gtggccgggc tttacgcgat ctcgccgctc gccgagttcc
     2341 cgttcctgta catgcgctac ataatgctga cgatcatgat cctcgcaacg ctgggggcct
     2401 acgtgacgta cctgcggggc aggctgcgtt tcaggtacgg cagcccgggc aggagctacc
     2461 gggtagtgct gatcgcgacc ggggtcgtcg cggccgtgat cgcgctgaac atgggcttta
     2521 tgaagtccaa ttccagggtg ccttacacgg tatataatca gcccggatac accgtagaac
     2581 cgggtccccc gccaactggg ttcgaccagt gagggacggg tcccggggct ctagaagggg
     2641 cgaatcgtgg tagagctaca gaaagatccc ttaacccgga gcgactttct cggcttcggc
     2701 gttatgggcg ccattatggg ggccatcctg acgataccgc ccgtggcttt cgttctgagc
     2761 cccatcatca agacggacgt gctcggcgaa tcggacgttg gctcaggctg ggtggaggtt
     2821 gcctccgtcg cagacgtcgg cgtggaggag ccctcccact tcagggtaga gtttcccctg
     2881 cagcagacct acggggagag ggccatacag gacgagtttc caaactctgg caagtcgagg
     2941 atggagtata cgctggccaa cgccatctgg ctctcgtgga gggcccccat caagcagccg
     3001 ggccgccagg gctccaacgg cgacgtaatc ggcacccccg agcgacccgc catactggat
     3061 cagaagagtt cgggcttcac ggagtcggag cgcaaggaga tcatgagcaa gctgaacgtg
     3121 ctctccaatt cctgcgccca cctcggttgt cccgtgcggt ggatgagcga ccagcagctc
     3181 tacctgtgcc cttgccacgg cggactctac gacatcaacg ggggttgggt cggtgggccg
     3241 ccgccgcgcg gcatgtatcg ctacacggag gccgaggtca gggaaaacgg caagctctat
     3301 gtcaagcacg cttacgacat cgagccgggc ctagagaagc aagagcccta tgtggtctag
     3361 ggaggcagca tgataggcag gataaggaac caggtgacgg atgccagcca gttcatggtg
     3421 gactggatgg aacaccggac ggggatagtg accattacgg agcacttcct ctacgagccc
     3481 gtcccgaaac ggggggcctg gctctacacg cttggcagcg ccgtgctgtt caccataacg
     3541 ttgcagttcc tgaccggaat actgctcctc ttctactacg tgccaaccac cgacgacgcc
     3601 tggaactcgg tcagctacat catgaacgac gcctacttcg ggcaggttat ccgcggcatc
     3661 cactactggt cggccaactt cctgctggcg ttcatagggt tgcacatggg ccaggtgttc
     3721 ttctccggcg gctacaaggc gccgcgcgag ctgaactggg tggtcggcgt gatcctgctt
     3781 ctgctcgtga tcgggctcgc ctttaccggc tacctcctga ggtgggacca ggacggtttc
     3841 tgggcctccg tcgtcggcat gaagatcggc tcctactcgc cgttcgtggg gggatacgtg
     3901 atcaacttcc tgcttggagg cgacgcggtg gggccggcca cgctgtcgag gttcttcgcc
     3961 atccacgtgt ggctgcttcc cgcagccata gcgccgctta tcggggcgca cctgtacctg
     4021 ctcaggcgtc acggggtttt cggcagcgag ttcgagtaca cggacaggct ggcgaaactg
     4081 cacgagcgcc agcgcatgga ggactacgcc cgcatggaga cgggcgagga cgacggcgaa
     4141 ccctacgtag ggcgtcgcga ccgggccgac agggaaggct acgaaaggga cggagagtaa
     4201 cagcttttac cgagaacccg cacgcagggc tgtacgatgg ccccacaaga ctgagagaga
     4261 cgaggtgtag acgtgtacgc tccggcgact ggcaaagcca aaccggttga agaagaggta
     4321 atcaccgaag agaacgtgtt cgagcgtcct gacgaaacca taggtttctt cccc
//
LOCUS       DBNGVD010000558         3193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2333695, whole genome shotgun sequence.
ACCESSION   DBNGVD010000558 DBNGVD010000000
VERSION     DBNGVD010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2333695"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16CM_15000"
     CDS             <1..222
                     /locus_tag="AC16CM_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141189.1"
                     /translation="IGVRVPVLTLGPDVEDGLFAEQASDSSWPVEIGSLERAIVTTLG
                     PGAQGGPATIASPAGETPPALAPARYPIR"
     gene            429..1274
                     /locus_tag="AC16CM_15005"
     CDS             429..1274
                     /locus_tag="AC16CM_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR3141190.1"
                     /translation="MNQVKRTSPLSRYGVAVVVVGVALGTKLLLGPVIPQDAPFLLLF
                     GAIVVSAWYGGFGPGLLTVVLSGVTTDLLFLRPQDPFGTTALLLVFSLEGTLICALVE
                     AMRSASRRAETSKAEAERDHERLRRSEEHLRSLVEGMSDHAIFELDRQGRVASWNAER
                     LTGYPAGKAAGERFPTFFTAAGVEPGSPQYLLREASALGRSRKEGLVARRDGSRFRAD
                     VTATALHDAGESCVVSRWSRGTSRSRKRSKMRSSAASTCSSRCTRRGASSAPAWSGRR
                     LDPAC"
     gene            1721..2341
                     /locus_tag="AC16CM_15010"
     CDS             1721..2341
                     /locus_tag="AC16CM_15010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3141191.1"
                     /translation="MACDVHDGLAQSAAAAHQHLQAFARHNICSDRDREELDEALGLV
                     RDLVGEARQVIHDLRPTVLDDFGLAAAVRLQVASLRAEGLKIDLEESLGDERLPKEVE
                     TTLFRVAQEALTNIHKHARAEKARVVLDRPGSAVRLLISDEGRGFVLKRMSPNGRGEK
                     VGLSGMRERISLLGGKFEFHSEPGRGTTIEAEVGLPEAEEKRKHAG"
     gene            2331..3014
                     /locus_tag="AC16CM_15015"
     CDS             2331..3014
                     /locus_tag="AC16CM_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3141192.1"
                     /translation="MQVETWRLVLADDHHILRRGFRSLLSQEPDLEVVGEASNGLQAI
                     EICRRLRPDLILMDVRMPEMDGITATRRIKESQPGVGVLMVTMHENPDYLLEALDAGA
                     AGYVLKDAPGDRLISAVHRTLNGESPLNQELATLLLRRLANEKHHEPLPPPRADLDHQ
                     VGALTPRETEVLALLTTGRTNQQIAQLLNISKGTAKVHVERIIRKLEVSDRTQAAVRA
                     IEMGFSSSG"
BASE COUNT          603 a         1026 c         1080 g          484 t
ORIGIN      
        1 ataggggtcc gcgttccggt gctcaccctg ggccctgacg tagaagacgg cctgttcgcc
       61 gagcaggcat ccgactcgtc ctggcccgtg gagatcggct cgctcgagcg agcgatcgtg
      121 accacccttg gccccggcgc gcaaggaggg ccggccacca tcgccagccc cgccggagaa
      181 acacctccag cactcgcacc ggctagatat ccgatccgtt agcggggctc cggcccggag
      241 ggttctaagg gccgcctgcc acaccagaag aacacccata aatccccgaa aatacctcaa
      301 atggggtatg cctgaagccc cgcgaagccg cagaatggta acgtggttac gttcagatgc
      361 catagaggga ggctgggcgc accagcaaga gggtgaagcc tcggcacgtt cggcaggtac
      421 agggccagat gaatcaggtg aaacgcacct cgccgctctc gcgatacggc gttgctgtag
      481 tggtcgtcgg ggtggcgctg gggaccaagc ttctcctggg ccctgtgatc ccgcaggacg
      541 ctcccttcct gctgctcttc ggggccatcg ttgtgagcgc gtggtacggg gggtttgggc
      601 ctggcctgct gacggtggtc ctatccggcg tgaccaccga cctgctcttc cttcgtccgc
      661 aggacccgtt cggaacgacg gcgctgctgc tggtgttctc cctggagggc acgctcatct
      721 gcgctctggt ggaggctatg cgttcagcta gccggcgggc cgagacgagc aaggctgagg
      781 ccgagcgcga ccacgagcgc ctgcgcagga gcgaggagca cctgcgctcg ctggtggagg
      841 gcatgagcga ccacgccatc ttcgagctcg accggcaggg acgcgtggcg agctggaacg
      901 ccgagcgcct caccggctac ccggcgggca aggcagcggg cgaacgcttc cccacgtttt
      961 ttaccgcggc aggcgtcgag ccgggcagcc cgcaatacct gcttcgggag gcttcggccc
     1021 ttggccgctc caggaaagaa gggctcgtcg cccgccggga cggatcgcgg ttccgggctg
     1081 acgtaaccgc cacggcgtta cacgacgcgg gggagagctg cgtggtttct cggtggtcgc
     1141 gcgggacatc acggagcaga aaaagatcga agatgcgctc aagcgcagcc tcgacgtgca
     1201 gctcgcgctg cacgagacgg ggcgcatcct cagctccagc ctggagtggg aggagattgg
     1261 atcccgcctg ctaggggtgg taaggcgcgt ctcgaacctg acggcggtag tgattagcgt
     1321 accggatgag cgaggggagc tgagcatctg gcgttcgtcc ggcctagaag gcctctggcg
     1381 ccaggcacgt ttcgcccccg aggccctctc ggcccgacgg gaggtgctag aaaccaggga
     1441 gcaccgcctt ctcaggctgc gacggccgga cgaaccggag gagcatctgg tcggtctcta
     1501 cctgcccctg ctcgtgcagg acaggttgat cggcgtcctg gaagcatacg gccccgaggc
     1561 cctcgtcgac gaggggactg agacactgtt cagcctcgcc gttcaggcgg cgagtgccct
     1621 ggagaacgcc aggctctaca cggagctctc cgaacgcgag aaccagttga gagatctggt
     1681 ccgcaaactc ataagagccc aggaagaaga gcgccaccgc gtggcctgcg acgttcacga
     1741 cggcctcgcc cagagcgcgg cggccgccca ccagcacctc caggccttcg cccgccacaa
     1801 tatctgctcc gacagggata gagaagagct ggacgaggcg ctggggctcg tgcgggatct
     1861 ggtcggcgag gcgcggcagg ttattcacga cctccgtccc acggtgctgg atgacttcgg
     1921 cctggccgcc gccgtgcgcc tgcaggtggc ttcgctcagg gctgagggct tgaagatcga
     1981 ccttgaagag tccctgggcg acgagcgtct gcccaaggag gtcgagacca cgctcttccg
     2041 ggtcgcccag gaggccctga ccaacatcca caagcatgct cgggcagaga aggcgcgggt
     2101 ggtgctggac cgcccgggga gcgcggtgag gctcctgatc agcgacgagg gacgcgggtt
     2161 cgtactgaag cggatgagcc cgaacgggcg cggcgagaag gtcggcctct ccggcatgcg
     2221 cgagaggatc tccctccttg gcggaaagtt cgagttccac agcgagccgg ggcgtggcac
     2281 caccatagag gccgaggtcg gtctaccaga ggcggaagag aagcggaaac atgcaggttg
     2341 agacctggcg gctagtcctg gccgacgacc accacatcct gaggaggggc ttccggagcc
     2401 tgctgagcca ggagcccgac ctggaggtgg tgggtgaagc gtccaacggg ctgcaggcca
     2461 tcgagatatg ccggcgcctc cggccggatc tcatcctgat ggacgtccgc atgcccgaga
     2521 tggacggcat cacggcgacc cgcaggatca aggagagcca gccaggcgtg ggggtgctga
     2581 tggttacgat gcacgagaac cccgactacc tgctggaggc cctggacgcc ggcgccgccg
     2641 ggtacgtcct gaaggacgcc cccggagacc gcctcataag cgccgtacac cgtacgctga
     2701 acggcgaatc tcccctgaac caggaactgg ccacgctact cctgaggcgc ctggccaacg
     2761 agaagcatca cgaacctcta ccgcccccac gagcggacct ggatcaccag gtaggagcgc
     2821 taaccccacg tgagacggag gtcctcgcgc tcctgaccac cggaaggacc aaccagcaaa
     2881 tagcccagtt actcaacatc agcaagggaa ccgccaaagt ccacgtggaa cgcataatcc
     2941 gcaagctgga ggtctccgac cgcacccagg ccgccgtccg cgccatagag atggggttct
     3001 catcctccgg gtgagaagca cgctcgggct aacgcctacg ctttcggcta gtcgtcgctc
     3061 tccgtaacgg cagcttcggc accccacctt ccctacgtac cgatgaatta gggcggatat
     3121 gtgcaggcca cagcgagatc tggccgacag cgagacccga agggtcgaag cgttgctgac
     3181 aagcgacagc cgc
//
LOCUS       DBNGVD010000559         3643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1423760, whole genome shotgun sequence.
ACCESSION   DBNGVD010000559 DBNGVD010000000
VERSION     DBNGVD010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1423760"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="AC16CM_15020"
     CDS             complement(<1..689)
                     /locus_tag="AC16CM_15020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3141193.1"
                     /translation="MVMVQYQGAPSRAPLTESGAIPLAKGPPLSAREDPGRLRTTFDD
                     VAPLYDEVRPGYPERLFDDLAALSGTGEGARALEIGCGTGQATLPLARRGYRILGVEL
                     GVNLAAVTRAKLAEYPDARVLTCSFEEWPLEEGTFDLVVSATAFHWVDPRVRYRKSAR
                     ALRPDGSLALIWNRADPEGSSEGFFEALEEIHRREAPELAPERRPPRLDWDPDKAGEI
                     ERSGFFERPEEG"
     gene            1045..2130
                     /locus_tag="AC16CM_15025"
     CDS             1045..2130
                     /locus_tag="AC16CM_15025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3141194.1"
                     /translation="MRWTHALDEYLRHQTARDLFSGAVLITQGATRLYARTYGYASRA
                     WRVPNTLDTRFDTASVTKLFTAIATLQLVDAGLLDLDTPVMERLLLEDTALSKEINVF
                     HLLTHTSGIGDDADEEAGERYEDLWKTKPNYSVVETRDFLPQFANKPANFPPGEGCRY
                     CNCGYILLGLLIESITGMTYRDYVRAHVFAEAGMGASDFFRMDRANNDVAEGCDPVRD
                     ATGTIVDWKKNIYSYPPIGSPDGGAHVTVADLDRFLRSVIAGKLLSPELTTEFLTPRV
                     VHRTLPPTTTRDTWTQMYGYGLSFYVDQAGKVVCFEKDGVNVGVSAMIRHYPDQDMTL
                     ALLSNMEDGAWDPDWKIHEMVLEGQIT"
     gene            complement(2383..3336)
                     /locus_tag="AC16CM_15030"
     CDS             complement(2383..3336)
                     /locus_tag="AC16CM_15030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR3141195.1"
                     /translation="MRLDPAGGKKLDGGLSLGEAYELCRKVQKAHSRTYYFSTRLFPA
                     EVRPHVQALYAFMRYADEIVDTPHDLPLDAQLAVLEDFEAETLAAVSGEEVPNPILRA
                     YADTVHRCGIDPETITAFMKSMKMDTRVFRYPTFSDLETYTYGSAAVVGLMMCRVVGV
                     ADERADHHAEALGVAMQLSNFLRDVGEDWRRGRVYLPMEDLARFGYGEQDLASGAVDE
                     RFVALMRFEIDRARGLYEVADEGMKYIPRGRRFPVVVARELYAAILDRIEAQNYDVFS
                     RRAQVSRPAKLVCAVRCAAGDPGEISARVRQRRTFRERTTR"
     gene            complement(3333..>3643)
                     /locus_tag="AC16CM_15035"
     CDS             complement(3333..>3643)
                     /locus_tag="AC16CM_15035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3141196.1"
                     /translation="AFKEKVYGLLERRCGLDRGRVVYEKVRTPEDWRDEYNLEEGAAF
                     GIGHGIFQVGYFRPPMVSKAIEGMYFVGASTRPGTGVPLVTIGARLVADRIGREVGGP
                     "
BASE COUNT          651 a         1188 c         1136 g          668 t
ORIGIN      
        1 ccctcctcgg gcctctcgaa gaaccccgag cgttctatct cgcccgcctt gtcaggatcc
       61 cagtccaggc gaggcggcct ccgctccggg gcgagctctg gcgcctcgcg acggtggatt
      121 tcctcgaggg cttcgaagaa accctcgctg ctgccttcgg ggtctgcacg attccagatg
      181 agcgcgagcg aaccatccgg ccgcagtgct cgagcgctct tgcggtagcg gacccgggga
      241 tcgacccagt gaaacgccgt ggccgagacg acgaggtcga aggtcccctc ctcgagcggc
      301 cactcttcga aggagcaggt gagcacccgc gcgtccgggt actcggccag ctttgcccgg
      361 gtcacagcgg caaggttcac gccgagctct acgcccagga tgcggtagcc ccgccgcgcg
      421 agaggtagcg tcgcctggcc cgtcccgcag ccgatctcga gcgccctcgc tccttcccct
      481 gtgcccgaaa gggcggccag atcgtcgaag agccgttcgg ggtagccggg acggacctcg
      541 tcgtagaggg gagccacatc atcgaaggtg gttcgcagcc gccccgggtc ttctcttgcg
      601 ctcagcgggg gtcctttcgc gaggggtatg gctccgcttt cggtcagggg agcgcgggaa
      661 ggtgctccct ggtattgaac cataaccatg agatcttctc ccgtacgaga ccttcgagct
      721 cgagtttgtg gggcagcggt gccaagcggc gtattttcgt gcgcatcacg agggcgcgtg
      781 aactcccgta ggggatgagg ggccatgctg ccccgcggcg gttctcgcgc tcccgagagg
      841 agccagttct tcaacacgct gcggtcttct ccggagtacg cctcgccaag cacggggcct
      901 acgcaagttg cactagtaat tgctacgatc gtccgaaggt gcgaccatgt tgccccgaga
      961 gggtactgtg gccctcaggt tctccacacg gcgacgccgc gagggttacc ggggcacgac
     1021 gaggtagcgc tcgggaagga catcatgcgt tggacccacg cactagacga gtatttacgg
     1081 caccagaccg ccagggacct gttctccggc gcggtgctca tcacgcaggg cgcaacccgg
     1141 ttgtacgccc gcacctacgg ttacgccagc cgcgcttggc gcgtacccaa caccctggac
     1201 acgcgcttcg atacggcgtc ggttaccaaa ctattcaccg caatcgcgac gttacagctg
     1261 gtcgacgcag gactgttgga cctggatact cccgtgatgg agcgcctgct cctggaagac
     1321 acggcgctct cgaaggagat caacgtcttt cacctgctga cccatacctc tggtataggc
     1381 gacgacgccg acgaagaggc gggagaacgc tacgaggacc tgtggaagac gaagcccaac
     1441 tattctgtcg tcgaaacccg cgacttctta ccccagttcg cgaacaaacc ggccaacttc
     1501 cccccgggag aagggtgccg ctactgcaat tgcggctaca tcctcctggg tctgctgatc
     1561 gaatcgatca ccggtatgac gtaccgtgac tatgtccgcg cccacgtgtt tgccgaagcg
     1621 gggatgggtg cctccgattt tttccggatg gaccgcgcca acaatgacgt tgccgaagga
     1681 tgcgacccgg ttcgcgacgc aactggcact atcgtagact ggaaaaagaa catctactcc
     1741 tacccgccga tagggtcgcc ggatggcggt gcccatgtca cggtcgccga cctggaccgc
     1801 ttcctgcggt cggtaattgc gggcaagcta ctttcgcccg agctgaccac agagttcctc
     1861 acacctcgag tcgtgcatcg aaccttgccg ccgaccacca cgagagatac ctggacgcag
     1921 atgtacggat acgggttgag tttctacgtt gaccaggcgg gcaaggtggt ctgcttcgaa
     1981 aaagatgggg tgaacgtcgg cgtcagtgcc atgatccgcc attatccaga tcaggatatg
     2041 accctagcat tgctatcgaa catggaagat ggcgcgtggg atcccgattg gaagattcat
     2101 gagatggttc ttgaaggcca gatcacgtaa ccccgcaaaa atcctaccct gtcgttcctc
     2161 agacatgccc cgggctcgag aggcacgagc gtagagggtc gaaaggggtg caaccttgta
     2221 tggctgagcc cctgtcctcc tgcccctggt cagctgcagc ggattgcaga gatttagacg
     2281 ctcttcggac ggagaatgct ggtctcggca ttcggctttt cgttctgtcc gagggctgaa
     2341 agtcgactgt tgacggcttt aaacccgctg ccccgtaggg gctcagcggg tcgtgcgttc
     2401 gcgaaaggtt ctccgctggc ggacgcgggc ggagatttca cccgggtcac cggcagcgca
     2461 cctgacagca cagaccagct tcgccggcct cgagacctgc gcccggcgcg agaacacgtc
     2521 gtagttctgg gcttcgatac ggtcgaggat cgccgcatac agctcacggg cgaccaccac
     2581 gggaaaccgc ctgcccctcg ggatgtactt catcccctcg tcagcgacct cgtagagccc
     2641 gcgtgcccgg tctatctcga agcgcatcag cgcgacgaag cgctcgtcaa cggcgccgga
     2701 ggcgaggtcc tgctccccgt agccgaagcg cgccaggtcc tccatcggca ggtacacccg
     2761 accgcgcctc cagtcttccc cgacgtcgcg aagaaagttg gagagctgca tggcgacgcc
     2821 gagggcctcg gcgtgatggt cggctctctc gtcagccacc ccgaccaccc tgcacatcat
     2881 gaggccgacc acggcggcgc tcccgtaggt gtacgtctcc aggtcggaga atgtagggta
     2941 gcggaacacg cgggtgtcca tcttcatgct cttcatgaag gcggttatgg tctccgggtc
     3001 gatgccgcac cggtgcaccg tgtcggcgta ggcgcgcagt atggggtttg ggacctcctc
     3061 ccccgagacg gcggccaggg tctcggcctc gaagtcctcg agcacggcca gttgcgcgtc
     3121 cagcggcagg tcgtgggggg tgtccacgat ctcgtcggcg tagcgcatga aagcgtagag
     3181 ggcttgcacg tgcggcctga cctcggcggg gaacaggcgg gtagagaagt agtaggttct
     3241 gctgtgcgcc ttctgtacct tgcggcacag ctcgtaggcc tcgccgagcg agagcccgcc
     3301 gtccagcttc tttccgcccg cagggtcgag cctcacgggc cgccgacctc ccgtcctata
     3361 cggtcggcca cgagcctcgc gccgatggtg accagcggta cccccgtgcc cggcctggtg
     3421 ctcgcgccga cgaagtacat gccctcgatc gcctttgaca ccatcggagg acggaaatac
     3481 ccgacctgga agatgccgtg cccgatgccg aacgcggccc cctcctccag gttgtactca
     3541 tcccgccagt cttccggcgt cctcaccttc tcgtagacca cgcgcccgcg gtcgagcccg
     3601 catcggcgct ccagcaaccc gtagaccttc tccttgaaag cgg
//
LOCUS       DBNGVD010000560         4333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_514404, whole genome shotgun sequence.
ACCESSION   DBNGVD010000560 DBNGVD010000000
VERSION     DBNGVD010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_514404"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16CM_15040"
     CDS             <1..287
                     /locus_tag="AC16CM_15040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3141197.1"
                     /translation="DLGCLGKGETVPPFEKAAFGAEEGEIVGPVKTQFGYHLLEVTDK
                     KPQQVRSLDEVESQIRAQLASQKQSEAFSKWLEEQKKKRDVKYLEGYKPA"
     gene            534..1834
                     /gene="eno"
                     /locus_tag="AC16CM_15045"
                     /pseudo
     CDS             534..1834
                     /gene="eno"
                     /locus_tag="AC16CM_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            1837..3261
                     /gene="pyk"
                     /locus_tag="AC16CM_15050"
     CDS             1837..3261
                     /gene="pyk"
                     /locus_tag="AC16CM_15050"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3141198.1"
                     /translation="MRRTKIVATLGPATSTEESITSLIRAGVDVTRFNFSHGEHSMHL
                     KNAEIVRRVAAETGRNVAILQDVQGPKIRTGNVEDGTELVEGSRVTLAAGDFSGDASR
                     LSTSYENLAEDVKPGDRLLIDDGLIGLRVESIENGEVVCEVLEGGPVSSHKGLNFPDS
                     TLSIRGLTPKDVEDLRFGMEELHPDWVAMSFVRTADEVREVKERIREFGGNAPVISKI
                     EKHEAIDNIEDIIAASDGIMVARGDLAVELSAERVPIEQKRMIARCRRLGKPIIVATQ
                     MLDSMIRNPRPTRAEVSDVANAIFDRADAVMLSGETAVGRFPEGSVQEMERICRAAEG
                     AINYGRDIPASASWGRGDRYDAVTHAACELAEDLGAEAILTSTQTGLSCIRVARFRPP
                     NKILAISPIEHTVRMLALVWGVTSIYADQAGSLEDRFGGAIEAAREAGHLKNGDEVIL
                     TGGTAGSVPGSTNVVEVSTVGEDV"
     gene            3310..3567
                     /locus_tag="AC16CM_15055"
     CDS             3310..3567
                     /locus_tag="AC16CM_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3141199.1"
                     /translation="MVVVLYAAFLGLLLASYVQPLQDIMHYRDEIPAMEQKLQKTTTQ
                     NTARERAIEELQTPAGIERAAREHYGMIRPGEQVYIVPKTR"
     gene            3698..4144
                     /locus_tag="AC16CM_15060"
     CDS             3698..4144
                     /locus_tag="AC16CM_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HIR3141200.1"
                     /translation="MAESSRDRDAVARQLGRSPRPFRVAARCPFGLPSVIENERTREM
                     PTSFWATCPSLVAAISRVESTGGVREAREVLGEPVVEEVHDEHRSRYGTRVAGVREEA
                     TGSVKCLHAFTALHISGAIPNPVAEWTLRRTGNPYPKESCCTKREG"
     gene            4170..4253
                     /locus_tag="AC16CM_15065"
     tRNA            4170..4253
                     /locus_tag="AC16CM_15065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4204..4206,aa:Leu,seq:taa)
BASE COUNT          864 a         1337 c         1445 g          687 t
ORIGIN      
        1 gcgatctcgg ctgcctcggc aagggcgaga ccgttccgcc cttcgagaag gcggccttcg
       61 gcgccgaaga aggcgagatc gtcggccccg tcaagactca gttcggctac catctgctgg
      121 aggtcactga caagaaaccc cagcaggtcc gttccttgga cgaggtcgag tcgcagataa
      181 gggcccagct cgcttcccag aagcagtcag aagccttcag caagtggctg gaggaacaga
      241 agaagaagcg tgacgtgaag tacctcgaag gatacaagcc cgcgtaggca tcaggtttca
      301 ggcttcaggt agaccggtgg cgcggcggat cggcagcgcg tagagttagc gcggagcggt
      361 cagccatcag ccgtcagcta cggaagttga cgtcttcggg gagaagccaa caggacccta
      421 gtgaaaaggg cccgctgcac aggtttgggg aacgtgggtg ttcgggcaca ccgttaatta
      481 ctggtcagct ggctgctgaa agctgatagc tagaagctaa aaaggggagg atgatgactg
      541 agatcgtcgc ggtccacggg cgtgagattc tggactccag ggggaatccc accgtggagg
      601 tggagcttgc cacggtgagc ggcttcgtcg gccgcgcctc ggtgccatcc ggggcctcga
      661 ccggccagaa cgaggccgtc gagctcaggg acggggagcg agaccgttat ggtggcaagg
      721 gcgtctccaa cgccgttctg aacgtggaga ccgagcttgc gcaggcggta gtcgggctgg
      781 acgcgacaga ccagcggacc ctggacctca ccatgctaga ggccgacgga acggagaaca
      841 agggtcggct gggggccaac gccatacttg gcatctccct ggcagctgcc aaagcctcgg
      901 ccgaggccgc cgcgctcccc ctgtaccgtt accttggcgg ggctggcgct tacacccttc
      961 ctgttccctg cgcaaacata atgaacggcg gcgcccacgc cgccaacaac gtggacttcc
     1021 aggagttcat ggtcgtaccc atcgggttcg agacctacca tgaggctttg cgttcggtgg
     1081 ccgaggtcta ccagggtctc aagaagctgc tggccgagag gggactctcc ggcggcatcg
     1141 gagatgaggc ggcttcgccc ccgacctgga gagcaacggc gccgcgctcg ggctcatcgc
     1201 cgaggccgtg gagcggagcg gttactcact gggcgaccag atcggtttcg cgctcgaccc
     1261 cgcggcatcc gagttctacg aggacgggca gtacgacctg gccggcgagg gcaagaagct
     1321 ctcccgacag gagatggtgg actactacgt cggcctgtgc gaccagtacc ccatcctcag
     1381 catcgaggac ggcctggccg aggatgactg ggagggttgg gccatgatca ccgaacggct
     1441 cggtgacagg gtacagctcg tcggcgacga cctgttcgtt acgaacccga agatactcag
     1501 gcggggcata gaggagggcg tcggcaactc catcctcatc aaggtcaacc agatcggcac
     1561 tttgaccgag accttcgaga ccatggagct cgcccacaag tccgggtata ccaccatgat
     1621 cagccaccgc agcggcgaga cggaggacgc gacgatctcc gacctcgccg tggccgtgaa
     1681 cgccggccag atcaaaaccg gcgcgccggc ccgcggggag cgcgtggcca agtacaacca
     1741 gctcctgcgt atagaggagc tcctgggcga ggctgctttc tatcccggcc gcgccgcgtt
     1801 caacccgagg gtccagccgg gaagaggggt ctagagttgc gcaggacgaa gatagtagcg
     1861 acgcttggcc cggcgacctc caccgaggag tccataacgt ccctgatccg ggccggggta
     1921 gacgtaacga ggttcaactt ctcccacggc gagcacagca tgcacctgaa gaacgccgag
     1981 atcgtccgcc gggtcgccgc cgagacgggc cgcaacgtcg ccatcttgca ggacgtgcag
     2041 gggcccaaga tccggaccgg caacgtggaa gacgggacgg agctggtgga gggaagccgc
     2101 gtgacgcttg ccgccgggga cttttcgggc gacgcgagcc ggctctccac ctcctacgag
     2161 aacctggccg aggacgtgaa gcccggcgac cgcctgctca tagacgacgg cctcatcggc
     2221 ctgcgcgtgg agagcataga gaacggcgag gtggtctgcg aggtcctgga aggcggcccc
     2281 gtctcgtccc acaagggcct gaacttcccc gactccacgc tctccatcag ggggctcacg
     2341 cccaaagacg tggaggacct gcgcttcggt atggaggagc ttcatcctga ctgggttgcc
     2401 atgtccttcg tgcgtaccgc cgacgaggtc agggaggtca aggagaggat cagggagttt
     2461 ggcggtaacg cgccggtgat ctccaagatc gagaagcacg aggccataga caacatagag
     2521 gacatcatcg cggcctccga cggcatcatg gtggcccgtg gtgacctcgc cgtagagctc
     2581 tccgccgaga gggtgccaat agaacagaag cgcatgatcg cccgttgcag gcgccttggc
     2641 aaaccgatca tcgtggccac gcagatgctc gactccatga tccggaaccc ccgcccgacc
     2701 cgcgccgagg tctccgacgt ggccaacgct atcttcgaca gggccgacgc cgtgatgctc
     2761 tcgggtgaga cagccgtcgg gcgctttccg gagggaagcg tgcaggagat ggagcgcatc
     2821 tgccgcgccg cggagggcgc catcaactac gggcgtgata ttcccgcctc ggcctcctgg
     2881 ggacgcggcg accgctacga cgccgttacc cacgccgcct gcgagttggc agaagatctc
     2941 ggggccgagg ccatcctgac ctcgacccag acgggtctct cgtgcatcag ggtcgcccgc
     3001 ttcaggccac cgaacaagat cctggctata agccctatcg agcacacggt caggatgctc
     3061 gcgctggtgt ggggcgtaac ctccatctac gccgatcagg cgggctcgct ggaggaccgt
     3121 ttcggcgggg ccatcgaggc cgccagggag gccggacacc tcaagaacgg cgacgaggtt
     3181 atacttacgg gcgggacggc ggggtccgtg cctggctcga cgaatgttgt tgaggtgtcg
     3241 accgtgggcg aggacgtgta gagggagcat ggtgaaacag gggtgagcgt acccaggaga
     3301 ttgaggccgg tggtggtcgt gctatacgcg gccttcctgg gcctcctcct cgcctcctac
     3361 gttcaacccc tccaggacat catgcactac cgggacgaga tcccagctat ggaacagaaa
     3421 ctccagaaga ccaccacaca gaacactgcc agggaacgcg ccatagaaga gctccagacc
     3481 cccgccggca tcgagcgcgc cgctcgtgag cactacggca tgatcaggcc cggcgagcag
     3541 gtgtatatcg tgccgaagac acgatagcta ttagctgtca gccgtcagct gtcagccggg
     3601 tggaggaagg ccaaccgccc tcctacgtcc tggcccttgc cgatatactc tcgcgcgatg
     3661 aagccccgaa aacggaaaag ctgatagctg acgcctaatg gctgaaagct ctagagatcg
     3721 ggacgctgtg gcgcggcagc ttgggcgttc gccccggcct tttcgcgtgg ctgcacgctg
     3781 tccgttcgga ttgcccagcg tgatcgagaa cgagcgcacg cgcgagatgc cgacaagctt
     3841 ctgggctacc tgtcccagcc tcgtggccgc catctctcgg gtggagtcga ccggtggcgt
     3901 cagggaggcg cgtgaggtgc tcggcgagcc cgtggtggag gaggtccacg acgaacaccg
     3961 atcccgctac gggacgcgcg tcgccggtgt gcgggaggag gcgacgggaa gcgtgaaatg
     4021 cctgcacgcc ttcacagccc tccacatctc tggcgccatt cccaaccccg tggccgagtg
     4081 gaccctccgg aggacgggca acccctatcc caaagaaagt tgctgtacga agcgggaagg
     4141 ctaactgcta tactcgcggc ctgaccgctg ggggcgtggc ggaatcggca gacgcaccgg
     4201 acttaaaatc cggagaccgc aaggtcgtgt gggttcgagt cccaccgccc ctacttgtca
     4261 aagttcctgc aaaagggtgg aaaccgaagg gcctccgact ttcctggagg ccctctcaca
     4321 agatctacca tgg
//
LOCUS       DBNGVD010000561         2348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1060769, whole genome shotgun sequence.
ACCESSION   DBNGVD010000561 DBNGVD010000000
VERSION     DBNGVD010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1060769"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16CM_15070"
     CDS             <1..525
                     /locus_tag="AC16CM_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141201.1"
                     /translation="YIAETQAYQAEVLKHATELFRRREYRPTGGTFAFMLNDPAPAIT
                     WSVVDWRRRPKAAYAALADAMRPVLVCAKYPDAQYTAGDELRLPIFVVNDLALHLGTL
                     GWTWEVRVDGNILDRGSGETSVPADSVVPLGTARTRLPEAGEGTLILGLSGDGVKETN
                     AYDFLVTSRSRRWP"
     gene            complement(497..1324)
                     /locus_tag="AC16CM_15075"
     CDS             complement(497..1324)
                     /locus_tag="AC16CM_15075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3141202.1"
                     /translation="MRHPRARIWPAVAGLAAGGTALAGARTALGRPVRSAGTLASAGV
                     GAAAAGLMVYEWLTPRSWLYGPVFWHARTEERVVALTFDDGPCHPHTEQLMDFFAGEG
                     IRATFFMVGNNVVREPNLAAEVASEHAVGNHTFTHPHLTWSWPSTIRDELSRGQEAIH
                     NATGTVPTIFRVPHGWYGPQVISGAAELGMHCIGWSVMAWDWDKPPVETIERRILHGA
                     FPGGVTLLHDGQDTDAYPKADRSHTTAAVPRIVETLREDGYRFVTIPELMAIDENGS"
     gene            complement(1321..>2348)
                     /locus_tag="AC16CM_15080"
     CDS             complement(1321..>2348)
                     /locus_tag="AC16CM_15080"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3141203.1"
                     /translation="LYRMSETRATHQETPSRATELYERWQQFTEQWAAGQDPAQNEAA
                     PTGTRPQRRDWLAIRALDRLIDQTGAKALVAPHFYGASALGRYKELHPEAFTSVVLTD
                     YVPHPLGVPTNLDLYIVADDAAAETVENIGVSADRIHPTGIPIDPAFELPADVPGVRR
                     DVLEFPDDDEDDLPVVIVMGGGLGGGHLEKVVTSLLEASAAMHLVVLCGSNTRDRDRL
                     QSVALARGRSATFLSFTDRVRDLMAASHVLVTKPGGMSCTESLASGLPQILFNPIPGQ
                     EEDNAAAMVRYGAGVMVDRTEDILGQAMKILTSRNHNRRMRSSAQAAHRPNSARNAAE
                     LLLERIP"
BASE COUNT          371 a          739 c          820 g          418 t
ORIGIN      
        1 tacatcgccg aaacgcaggc ctaccaggcg gaggtgttga agcacgctac ggagctcttc
       61 aggcgccgcg agtacaggcc gacgggcggt accttcgcct tcatgctcaa cgacccggcg
      121 cccgccatca cgtggtccgt ggtggactgg aggcgcaggc cgaaggccgc ttatgctgcc
      181 ctggccgacg cgatgaggcc cgtcctggtg tgcgcaaagt atccggacgc gcaatacacc
      241 gcgggcgacg agctgaggct gcccatcttc gtggtgaacg atctagcgct gcacctcggt
      301 acgcttgggt ggacctggga ggttagagtc gacgggaaca tcctggaccg ggggtctggc
      361 gagacgagcg tgcctgccga ctccgtagtg ccgttgggaa cagccagaac gaggcttcca
      421 gaagcgggag agggaacttt gatccttggg ctctccggag acggcgtgaa ggagaccaac
      481 gcctacgact tcttggttac gagccgttct cgtcgatggc catgagctcg gggatcgtaa
      541 cgaaccggta gccgtcctcc cgcagcgtct cgacgatgcg tggtacggct gccgtggtgt
      601 gtgagcgatc ggccttgggg taggcgtccg tgtcctggcc gtcgtgcagc agggtcacgc
      661 ccccgggaaa ggccccgtgc aggatgcgcc gttctatggt ctcgaccggt ggcttgtccc
      721 agtcccaggc catcacggac cagccgatgc agtgcattcc cagctctgct gcacccgaga
      781 tcacctgcgg cccgtaccat ccgtgtggga cgcggaagat ggtcggtacc gtacccgtcg
      841 cgttgtgtat ggcctcctgg ccgcgcgaga gctcatccct gatggtgctc ggccagctcc
      901 aggtcaggtg cggatgggtg aaggtgtggt ttcccacggc gtgctccgag gcgacctcag
      961 cggccaggtt tggttcgcgc accacattgt tgccgaccat gaagaaggtg gccctgatgc
     1021 cctccccggc gaaaaagtcc atgagctgtt cggtgtgggg gtggcagggg ccgtcgtcga
     1081 aggtgagcgc gaccacgcgc tcctccgtcc tggcgtgcca gaagaccggg ccgtagagcc
     1141 aggagcgggg ggtgagccac tcgtatacca tcagcccggc ggctgctgcc ccgaccccgg
     1201 cgcttgccag cgtaccggca gaccggaccg ggcggccaag ggcggtacgc gcgcccgcaa
     1261 gcgctgtgcc gcctgcggca agtcccgcta cggccggcca gatcctcgcg cgcgggtgcc
     1321 tcacggtatg cgctccagga ggagttcggc cgcgttgcgg gccgagttgg ggcggtgggc
     1381 cgcctgtgcc gaggatcgca tccggcggtt gtggttcctg gaggtaagga tcttcattgc
     1441 ctgtcccagt atgtcttcgg tgcggtcaac cattacgccg gccccgtagc ggaccatcgc
     1501 tgcagcgttg tcttcctcct gcccagggat agggttgaac aggatctggg gcaaccccga
     1561 ggctaacgac tctgtgcagc tcatcccgcc cggcttcgtt acgagcacgt gggaagctgc
     1621 cataaggtcg cgtacgcggt cggtgaacga gaggaaggtg gcggagcgtc cgcgggccag
     1681 ggccacgctc tgcaggcggt cgcggtcgcg cgtgttcgag ccgcacagga ccacgaggtg
     1741 catagcggcg ctcgcctcga ggagagaggt tacgaccttc tccaggtgcc cgccgccgag
     1801 gccgccgccc atcactataa ccacgggcag gtcgtcctca tcgtcatccg ggaactcaag
     1861 cacgtcccgg cgtacgcccg gaacatcagc gggtagctcg aaggccggat ctatcgggat
     1921 cccagtcggg tgtatgcggt ccgccgacac cccgatgttc tcgacggtct ccgcggccgc
     1981 gtcgtcggcc acgatgtaga ggtcgaggtt cgtggggacg cccagggggt gcggcacgta
     2041 gtcggttaga accacggagg tgaaggcctc ggggtgcagc tccttgtacc tgccgagcgc
     2101 cgaggcgccg tagaagtgcg gggcgacaag cgccttggca ccggtctgat ctatcaggcg
     2161 atccagagcc ctaatcgcca gccagtcacg acgctgaggc ctcgtcccgg ttggggcagc
     2221 ttcgttttgg gctgggtctt gcccggcggc ccactgctct gtgaactgct gccaacgctc
     2281 gtatagctcg gttgcacggc tcggtgtctc ctgatgggtc gcccgcgtct ccgacatgcg
     2341 gtagagct
//
LOCUS       DBNGVD010000562         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1242321, whole genome shotgun sequence.
ACCESSION   DBNGVD010000562 DBNGVD010000000
VERSION     DBNGVD010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1242321"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..970)
                     /locus_tag="AC16CM_15085"
     CDS             complement(8..970)
                     /locus_tag="AC16CM_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3141204.1"
                     /translation="MTFGETMLRLSPPGHERLARAASLDVQVGGTESNVACALAQLGT
                     PASWVSRLPETALGRLVEARLRQAGVETSGIRWSQEGRLGLYFVEHGSDPRPTRVLYD
                     REGSAFAELDPDEFDWTDILEGSGIFHTTGITLALGPGPRRSLFDAAETARGAGVLVS
                     FDLNYRANLWSREQAERAYREVLPLVDVLFCGAAELAGLLGSETGSLRDMILRSREEW
                     GVGRVILTARSGGVLSYATLGGGEELLESREYAPRVVERFGAGDAAAAAFLHSLIGNR
                     DERYAVELAAAASALQHALPGDIASFTLDELESLAGGQEGDMLR"
     gene            complement(1042..1785)
                     /gene="yqeC"
                     /locus_tag="AC16CM_15090"
     CDS             complement(1042..1785)
                     /gene="yqeC"
                     /locus_tag="AC16CM_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR3141205.1"
                     /translation="MELHEALGITRGDVVAFVGAGGKSSAIIAVANGLRLAGMRVLVA
                     PTTKMTQGEADDVGPLVTSEDASELREKVEEAFAGSPAVVAGSGMISRNRVGGVDPGW
                     VGDLASLADVVLVEADGSRRRPIKGTADHEPALPDTATLVVGVANVRALGMPLDEEHV
                     HRPEVFSELTGVGPGQSVTPEAFATSIAGGSLAKVPPTARTAALITGVEPGRSMSDAS
                     VITRELWRRGVKNVVLAALADSPPRVWVP"
     gene            2009..2446
                     /locus_tag="AC16CM_15095"
     CDS             2009..2446
                     /locus_tag="AC16CM_15095"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3141206.1"
                     /translation="MDGRERVAEFGRELREGPEPSDRSIKEIVDAIRPQVQELVNKQV
                     ELAQTELTPVAKKGGLAAGLLAVAGAFMLVFLIIFSLAGVYLLSVFLSPWVSALIVSG
                     ILLLIGGILGGIGASILRGLDPKPHRTIATLQQNINWIKGQFR"
BASE COUNT          423 a          819 c          815 g          404 t
ORIGIN      
        1 gcaacgttca tcgaagcatg tccccctcct gaccgcccgc gaggctctcg agctcgtcga
       61 gcgtgaagct ggctatgtcg ccgggcaagg cgtgttggag cgcggaggcc gccgcggcca
      121 gctccacagc gtacctttcg tctctgttcc cgatcaggga gtgcaaaaac gcggctgccg
      181 cggcgtcccc ggccccgaaa cgctccacga ccctcggggc gtactcgcgg ctctccagaa
      241 gctcttcccc gccaccgagc gtcgcgtaag agagaacccc gccggagcgg gccgttagga
      301 tcacccggcc cacgccccac tcctcgcggg aacgcaagat catgtcgcgc agagagcctg
      361 tttccgagcc cagaagccct gcgagctcgg cagccccgca gaagagcacg tccacgaggg
      421 gaagtacctc ccggtacgcc ctctcggcct gctcccggct ccagaggttg gcccggtagt
      481 taaggtcgaa gctaaccaga acgccagccc cgcgcgcggt ctcagcggca tcgaagagcg
      541 accgtcgcgg cccggggccg agggccaggg tgataccggt ggtgtggaaa atcccgctcc
      601 cctccaggat gtccgtccaa tcgaactcgt ccgggtccag ctcggcgaac gcggagccct
      661 cgcggtcgta gagaaccctc gtgggcctgg gatccgagcc gtgctccaca aagtacagtc
      721 cgaggcgtcc ctcctggctc caccgtatcc cggaggtctc gacgccagcc tgtcgcagcc
      781 ttgcctcaac gaggcgcccg agggcggtct ccggcaggcg ggagacccag gaggcaggcg
      841 tgcccagctg ggccagggcg caggccacgt ttgactcggt acccccaact tggacgtcca
      901 gcgaggcggc ccgcgccagg cgctcgtgcc ccggcgggct caaacgcagc attgtctccc
      961 cgaaggtcac gacggtcctc tgaatcgctg gcttaccgcc agaggcggaa gatgctgtcg
     1021 ggttcgcgct cagcaggacc tctatggcac ccagacgcgg ggcggagagt cggcaagggc
     1081 ggcgaggacg acgttcttca cgccgcgacg ccagagttca cgggttatga cggaggcgtc
     1141 ggacatgcta cggcccggtt cgacgccggt gatgagggcc gcggtccttg ccgtgggcgg
     1201 gaccttggcg agcgacccgc cggcgatgga cgtggcgaag gcctcggggg taacgctctg
     1261 gccaggaccg acgcccgtga gctccgagaa cacctcgggg cggtggacgt gctcctcatc
     1321 caggggcatg ccgagagcgc ggacgttggc gacaccgacg accagggtgg ccgtatcggg
     1381 gagggcaggc tcgtggtccg ctgtgccctt gatcgggcgc cggcgcgacc cgtcagcctc
     1441 caccaggacc acgtcggcca gggaggcaag gtcgccgacc cagcccgggt caactccgcc
     1501 gaccctgttc ctggagatca tcccggagcc ggccacaacc gccggggatc cggcgaaagc
     1561 ctcctcgacc ttctcgcgca actcgctggc gtcttccgag gtgacgagcg gccctacgtc
     1621 gtccgcctcg ccctgcgtca tcttcgtggt cggggcgact agcaccctca ttccggccag
     1681 ccggaggccg ttcgcgacgg cgatgatcgc gcttgatttg ccgccggctc ccacgaaggc
     1741 gaccacgtcc ccgcgggtga tgccgagcgc ctcgtgcagt tccaaaacag ctcctatccg
     1801 cgctggaggt tgcccttcct tgtacacccg aattcatgtt acatcatcga tacaccacgc
     1861 gtgctttatg gcatgttgga tactaggatc tggtgtagga tgagaaggat ggtccctggc
     1921 aagcccggct gattacgtga ggtgcggtag gggtaattag gagcaagaag acttgtagcc
     1981 cctggtattg gagcaaagga gcgtggggat ggatgggcga gaacgggtag cggagtttgg
     2041 tagggagctt agagagggtc ccgaaccttc cgaccggagc atcaaggaga tcgtcgacgc
     2101 catccggccg caggtccagg agcttgtaaa caagcaggtc gagctggccc agacggagct
     2161 gacaccggtg gccaagaagg gcggcctcgc cgccggcctt cttgccgtgg ccggcgcatt
     2221 catgctcgtc ttccttatca tcttctctct cgccggcgtc tacctgttga gtgtctttct
     2281 gtccccgtgg gtctcggccc tcatcgtcag cggcatactg ctgctcatcg ggggcatact
     2341 gggcgggatc ggcgccagca tcctgagggg tcttgatccc aagccccacc gcaccatcgc
     2401 cacgctgcag cagaacatca actggataaa ggggcagttc aggtaatgag cgagattccg
     2461 g
//
LOCUS       DBNGVD010000563         5077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_695403, whole genome shotgun sequence.
ACCESSION   DBNGVD010000563 DBNGVD010000000
VERSION     DBNGVD010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_695403"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC16CM_15100"
     CDS             <1..681
                     /locus_tag="AC16CM_15100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3141207.1"
                     /translation="GDLSRAEAAFRRGLEDCPGSPEIRDELAAHLLALGKNLPEALKL
                     SEEAVALGRDEIRHLITLGEARLAIGDDKGSAEAYEVVISLDPENPEAHLELGILNER
                     EGHMERAEEHFVESLKADPANPRALYSYASLYYTADDLEVAEEILERAIAADARYSPA
                     LSALASIQARRGEFGDALDYIERAVDAGENDVEHFRSALEFAPLHADPRFRTLLSRMV
                     REERNGQG"
     gene            685..897
                     /locus_tag="AC16CM_15105"
     CDS             685..897
                     /locus_tag="AC16CM_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141208.1"
                     /translation="MQPSLAFGILLSITGLACLAFSAYALLRGGKDQRGGIGPISERG
                     IHLVAGIRMLVIGALSLAAGIYLLLS"
     gene            complement(992..1594)
                     /locus_tag="AC16CM_15110"
     CDS             complement(992..1594)
                     /locus_tag="AC16CM_15110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3141209.1"
                     /translation="MSRVVEEIEALVARAGTHPVYGYTHCLRVYALAEDLAAQEDVSH
                     DPEILRVASLLHDVGLYKAYAMREPADHAKRSALVARRILQDWDFPPQASQAVIEAIE
                     FHPPGVAGGTASETILLKDAVGLDYLGAIGVSRMLAMVGTEDDVPDIRTAIWNAESLR
                     QKIPGLLILQSSKDLAARRIHEMEYFLEDLRDATDKLKLL"
     gene            complement(1908..2204)
                     /locus_tag="AC16CM_15115"
     CDS             complement(1908..2204)
                     /locus_tag="AC16CM_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141210.1"
                     /translation="MSEIFPMEIRAMAIALFYSIATAIGGISGPVLFGRIIGTGNEGE
                     LFIAYLVGAAFMIFAAVIELVLGIRAEGESLENVATPLTAIEEATGTSAASGSA"
     gene            complement(2341..3447)
                     /locus_tag="AC16CM_15120"
     CDS             complement(2341..3447)
                     /locus_tag="AC16CM_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3141211.1"
                     /translation="MGGSFKIGRAFGIDVKVHWSFFLLLAFFGFLAFRDSGSLLSALV
                     TVGLVVALFFCVLLHEFGHSLVAIRLGSEVQDITLLPIGGLARLKSMPERPIDEVKVA
                     VAGPLVNVVLAPVFFGLAVLFGGDLSTPTDIISGPESVGQAFVALGIINVALVIFNMI
                     PAFPMDGGRVLRGLLATRLGPVRATDISSTVGQFFAILFILGGFLSQNFLLAIVGFFV
                     FLGASGEAQLVRQREQMRGLAVSDVMGTKGRTETVTPYHNFGQVLDSVIHGYQEDFPV
                     VDEDGSLVGIITRNEIMAAAHSPNRYATVRDLMQTEFPTISPDADLFQDGNRILQESG
                     LRALPVVKDGNLVGMLTVDDVGQAALLRDLRKAP"
     gene            3513..>5077
                     /locus_tag="AC16CM_15125"
     CDS             3513..>5077
                     /locus_tag="AC16CM_15125"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HIR3141212.1"
                     /translation="MEERTETYRLVTVAEDLAAIAKTLRNAEAIGVDIETTALSPRDG
                     TVRLLQLATPDETFVIDVFEVPDLSPLGEVLENGPVKVGHNLKFDFQFLQALHGISLS
                     PIFDTMLAAQVLSGGNYAASYSLDAVAERFLDEDLDKSEQRSDWSGELSASQLEYAAR
                     DARILVPLRERLAEALEAEELSRISGIEFAAVASIAEMELAGVKLDVARWKELEITVR
                     ERRDRAAEHLESFFPPPEGVLPLEGLGPRLNLNSPKQITDAFKSLGIDLPDTKVWTLL
                     KVDHPAAKALLDYRELQKKLGTYLETYPNFISPKTGRIHANFLQCRVPTGRLACASPN
                     IQQIPHEDEFRRCFVAEDGYVLVIVDYSQIELRILAEVSDDPAFVEAFRRGDDLHRMT
                     AATMYSVPMEEVTKDQRSDAKRINFGLMYGRGARSLSAQLGTDEERGRQLIDEYFANY
                     PKVQRFLQRTANRAMRDRTLRTLAGRVRKFGNDPVADDRGAMRREAMNYPIQGSSADI
                     AKLALVYVRDDLEG"
BASE COUNT         1000 a         1672 c         1586 g          819 t
ORIGIN      
        1 ggggacctct ccagggcaga ggccgccttc cgacgcggcc tggaagattg tcccgggtcg
       61 ccggagatca gggacgagct cgccgcccac ctgctcgccc tcggcaagaa cctgcctgag
      121 gctctcaagc tctcggagga ggccgttgcg ctgggccggg atgagatccg ccacctcata
      181 accctaggag aggcccgcct ggccatcggg gacgataaag gatctgccga ggcctacgag
      241 gtcgtgatct cactcgaccc cgaaaaccct gaggcccacc tggagctggg catcctgaac
      301 gagcgcgagg ggcacatgga gcgggccgag gagcacttcg tggagtcgct caaggccgat
      361 cccgccaacc cccgggccct ctactcctac gccagcctct actacaccgc cgacgacctg
      421 gaggtggccg aagagatcct ggagcgggcc atcgcagcag acgcccgcta ctcacccgcc
      481 ctctcagccc tggccagcat ccaggcccgc cgcggcgagt tcggggacgc cctcgactac
      541 atcgagcggg ccgtggacgc cggcgagaac gacgtcgaac acttcaggag cgccctcgaa
      601 ttcgccccct tgcacgccga cccccgcttc cgcaccctac tgtcgcgcat ggtaagagag
      661 gaaagaaacg gacagggcta gggcgtgcaa ccctccttgg ctttcggaat actcctgagc
      721 atcaccgggc tcgcctgcct ggcattcagc gcctacgccc tcctgcgagg cggcaaagac
      781 cagaggggcg gaataggccc catctccgaa cgtggaatcc atctcgtcgc cggcatcagg
      841 atgctggtga taggagccct gagcctcgcc gccggcatat atctcctgct tagctagaac
      901 gacttgccag aagagcgggc ggtcagcttc cagctataga taagaagctg aaggcagatc
      961 gctgacagct gtcagcttct tgagtaccgc cctagagaag cttcagcttg tccgtggcat
     1021 ctcgcaggtc ttccaggaag tattccatct cgtggatgcg gcgggcggcc aggtctttgc
     1081 tgctctggag gatcaggagg cccgggatct tttgcctgag gctctctgcg ttccagatgg
     1141 ccgtacgtat gtcggggacg tcgtcttcgg tgcccaccat ggccagcatg cgggagacgc
     1201 cgatggcacc gagatagtcg aggcccacgg catccttgag caggatggtc tcggaggcgg
     1261 tgcccccggc gacacccggc gggtggaact ctatggcctc tatcaccgcc tggctggcct
     1321 gcggcggaaa atcccagtcc tggagaattc gccgggccac cagcgccgag cgcttggcgt
     1381 gatcggccgg ctccctcatg gcgtaagctt tgtagagccc cacgtcgtgc aggagggacg
     1441 ccacacgcag tatctccgga tcatgggaga cgtcctcctg ggccgcaagg tcttccgcca
     1501 gcgcgtagac cctgaggcag tgggtgtacc cgtagaccgg atgtgtcccc gccctggcga
     1561 cgagcgcctc gatctcctcc accacgcggc tcatgcggag agtctagcga atagacctgt
     1621 gcttcgcagc acgctccggc taacgccttc gctcgtcagc acgccggctc cgcaccgctg
     1681 cgcccggctc cgcctagagc tcgctttcag ccagatctcg ttgcggcctg caccctgcca
     1741 ccctagagac tcgctgcccg gaaaagttgg aatttcgaag ccaccgatgc gaatcgagac
     1801 gaccggccga aagcgaggcg aagccgaagc gttgccgacg agcgacagcc tccaggctgg
     1861 agcgtgctga caagcggagg cgttagccgg agcgtgcgga tccgccgtca ggcggatcca
     1921 ctcgcggcgc tggtgcccgt ggcctcctca atggccgtca gcggcgtggc aacgttctcc
     1981 agtgattctc cttcggccct tatgcccagg acgagctcga tcacggcggc gaagatcatg
     2041 aaggctgcgc cgaccaggta ggcgatgaac agttctcctt cgttgccggt gccgatgatc
     2101 ctgccgaaca gaacgggacc cgagataccg cctatggctg tggctatgga gtagaagagg
     2161 gctattgcca tggcccggat ctccatggga aagatctcgc tcaccgtcag gcctctacga
     2221 ccgcccctca gcagaccagt agagaagccc ggcaccgcac cacaaactcg tcacaacaca
     2281 ctcctgtagg agcggttcgc gaaccgcccg acggcgtcac ccctccccac gaacgacagg
     2341 ttacggcgcc ttgcgcaggt ccctcaaaag ggctgcctgg cccacgtcat ccacggtcag
     2401 catcccgacg aggttaccgt ccttcacgac gggtaaggcc ctcaggccgc tctcctgaag
     2461 gatacggttg ccgtcctgga ataggtcggc atcgggtgag atggttggaa actccgtctg
     2521 catcaggtca cgcacggtag cgtagcggtt tggggagtgg gccgcggcca tgatctcgtt
     2581 gcgcgtgatg atgccgacca ggctgccgtc ctcgtcaacg acgggaaaat cttcctggta
     2641 gccgtggatg acggagtcga ggacctggcc gaagttgtgg tagggcgtga cggtctccgt
     2701 cctacccttg gttcccatca catccgagac ggcgaggccg cgcatctgct cccgctgcct
     2761 tacgagctgg gcctccccgc tcgcgccgag gaacacgaag aaccccacga tggcgagcag
     2821 gaagttctgg ctaaggaacc cgccgaggat gaacaagatg gcgaagaact gcccaaccgt
     2881 ggaggagatg tccgtggccc gcacgggccc gaggcgcgtg gcgagcaggc cgcgcaacac
     2941 acggccgccg tccatgggga aggcggggat catgttgaag ataacgagcg ccacgttgat
     3001 gatgccaagg gcaacgaagg cctgcccgac cgattcgggc ccggagatga tgtccgttgg
     3061 ggtggagagg tcgccgccga agaggacggc aagcccgaag aataccgggg ccagcaccac
     3121 gttgacgagc ggcccggcca cggcgacctt gacctcgtcg atgggcctct ccggcataga
     3181 cttcagcctg gccagcccac cgatgggcaa cagcgtgatg tcctgcacct cgctcccaag
     3241 ccgtatggca acgagcgaat gcccgaactc gtgcaacaag acgcagaaaa acagcgccac
     3301 cacaaggccg acggtaacaa gagcgcttag caggctcccc gagtctctga aggcgaggaa
     3361 accgaagaag gccaggagca agaagaagct ccagtgaacc ttcacgtcta tcccgaaagc
     3421 gcgtcctatc ttgaaggaac cacccatcgg tctgattcta ccccacgcgc cagagggcga
     3481 tgtgatctca aacgccctgc tagaatcgaa gggtggaaga acgcaccgaa acctaccggc
     3541 tcgttactgt ggcggaagat cttgccgcca tcgcaaagac gctacggaac gccgaggcca
     3601 tcggggtgga catcgagacc accgccctct ccccccgaga tggaacggtg cgcctgctcc
     3661 agctcgccac cccggatgag accttcgtca tagacgtgtt cgaggtcccg gacctttcac
     3721 cgctcgggga ggtgctcgag aacggcccgg tcaaggtcgg gcacaatctg aagttcgatt
     3781 tccagttcct tcaggccctg cacggcatct cgctctcgcc catcttcgac accatgctcg
     3841 ccgcccaggt tctctccggc ggcaactacg cggcatctta ctcgttggat gcggttgccg
     3901 agcgcttcct ggacgaggat ctagacaagt ccgaacagag gagcgactgg tcgggggagc
     3961 tgagcgcctc gcaactcgag tacgccgcgc gggacgccag gatccttgtg cccctgcgcg
     4021 agcggctcgc cgaggcgctg gaggcggagg agctctccag gatctccggt atagagttcg
     4081 cggccgtggc gagcatcgcg gagatggagc tggccggggt gaagcttgac gtggcccgct
     4141 ggaaggagct ggagataacg gtgcgcgagc ggcgcgacag ggccgcggag cacctggagt
     4201 ccttcttccc gccgccagaa ggcgtgctgc cgctcgaagg tctcgggccc aggctcaacc
     4261 tcaacagccc caagcagatc accgacgcct tcaagtccct gggcatagac ctccccgaca
     4321 ccaaggtctg gaccctcctg aaggtagacc accccgcagc gaaggcgctg ctcgattacc
     4381 gcgagctgca gaagaagttg ggcacctacc tggagaccta tcccaacttc ataagcccga
     4441 agacgggacg catccacgcc aactttctcc agtgccgcgt cccgacagga cgtctggcct
     4501 gcgcgtcacc gaatatccag cagataccgc acgaggatga gttccggcgc tgcttcgtgg
     4561 ccgaggatgg gtacgtgctc gtgatcgtcg actactcgca gatagagctt cgtatcctgg
     4621 ccgaggtctc ggatgacccc gcctttgtcg aggccttccg gcggggggac gacctgcacc
     4681 gcatgacggc cgccaccatg tacagcgtcc cgatggagga ggtcacgaaa gaccagcgct
     4741 cggacgccaa acgcataaac ttcggcctga tgtacggccg cggcgcccgt agcctctccg
     4801 cccagctcgg cacggacgag gagcgcggaa gacagctcat agacgagtac ttcgccaact
     4861 accccaaggt ccagcgcttc ctgcaacgca cggccaaccg cgccatgcga gacaggacgc
     4921 tcaggaccct cgccgggcgc gtgcgcaagt tcggcaacga ccccgtagca gacgaccgcg
     4981 gcgccatgcg gcgggaggcg atgaactacc ctattcaggg cagctcggcg gatatagcca
     5041 agctggccct ggtctacgtt cgcgatgatc tcgaagg
//
LOCUS       DBNGVD010000564         4945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_515982, whole genome shotgun sequence.
ACCESSION   DBNGVD010000564 DBNGVD010000000
VERSION     DBNGVD010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_515982"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="AC16CM_15130"
     CDS             complement(<1..779)
                     /locus_tag="AC16CM_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3141213.1"
                     /translation="MTEASSSGRVRALITGTGAVTPLGTGTENFWDAALHGKSGIGEI
                     TIFDPSPYPSRIAGECWDFDPTDFVSKKQARRMDRYAQLGIAAGLQAADSAGIVDLVR
                     ERPERVAIVMGSGIGGISTWEREYRVLNERGAGRVSPFLITMMVPNMAAGQLAIALGA
                     TGPTQCPVLACATGGEAIAEGLELIRRSDADVVIAGSSEAPITPLSMAGFCAIRAVSR
                     RNDDPTRASRPIDEGRDGFVMSEGAGAVILESEEHAVSRGA"
     gene            complement(776..1021)
                     /gene="acpP"
                     /locus_tag="AC16CM_15135"
     CDS             complement(776..1021)
                     /gene="acpP"
                     /locus_tag="AC16CM_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3141214.1"
                     /translation="MDREEVLGKIQEITADRLGVDEGDVTSDASFREDLEADSLDLVE
                     LIMELEEQFGMEIPDDEAEKITTVEEAVDYVMEHQAA"
     gene            complement(1094..1837)
                     /gene="fabG"
                     /locus_tag="AC16CM_15140"
     CDS             complement(1094..1837)
                     /gene="fabG"
                     /locus_tag="AC16CM_15140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3141215.1"
                     /translation="MQEGRVAVVTGGGRGIGRAVALRLASEGADVAISYRSNDETAEA
                     AAEEVRSKGVRCEIFKGDVASQDDVAALIKGVNDAFGRIDILVNNAGLTRDNLMMRMK
                     EEEFDEVISTNLKGTYLCTRAVLRPMIRARWGRIVNVSSVVGLVGNAGQANYAASKAG
                     IIGFTKSVAREVAQRNITANVVAPGYVETELTGGLSEEIKDQILAQVPVGRFGEAAEV
                     AEVVAFLAGEGAGYVTGQTVAVDGGMTMQ"
     gene            complement(1837..2286)
                     /gene="fabZ"
                     /locus_tag="AC16CM_15145"
     CDS             complement(1837..2286)
                     /gene="fabZ"
                     /locus_tag="AC16CM_15145"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3141216.1"
                     /translation="MKAPLGPAEIRQLIPHRYPFLLVDRIDELEPGVRASGVKNVTQN
                     EPFFQGHFPEYPVMPGVLIIEALAQVGAVGVMSGGDHGDKLALFAGIDGVRFRRQVVP
                     GDVLTLEVEIVRLKGRVGRGKGRALVGDERVCEAELMFAFVERGEIP"
     gene            complement(2283..3095)
                     /locus_tag="AC16CM_15150"
     CDS             complement(2283..3095)
                     /locus_tag="AC16CM_15150"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3141217.1"
                     /translation="MNRAVVFPGQGSLGGETSGSVRAAVLEYLGDREVPYQLAVFANS
                     VDLLYRLRDAGIDGDVVAGHSLGEYAAAHAAGSLGLEDGVRLVAERDRLMNEASRKTP
                     GGMVALIGADPDAVVEAAEGSGGAVVAANFNSPRQVVISGRLDALEEVTEGLKGRKVP
                     LRVAGAFHSPLMAEAAREMDVLLDGVEFGEPGIPIIGATEGTLLTTANGVRNTLRNQM
                     LSPVRWVSVVERLLELGVREIYEAGEDGTLTRMLRDFKRRDVTSKTVGEVLA"
     gene            complement(3105..4049)
                     /locus_tag="AC16CM_15155"
     CDS             complement(3105..4049)
                     /locus_tag="AC16CM_15155"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3141218.1"
                     /translation="MNVAAGKIIGVGRALGSRVVTNADLETVLDTSDEWISTRTGIKQ
                     RRFVAEGENTATLAIEASRKALEHAGVDGGSVDLVICGTSTAPESMPSVACLVGEAVG
                     VPGAGAMDLAAACAGFSYAASVASSMIRAGAASRILLIGADAMSTIVDPNDRSTAVLF
                     GDGAGAVVLDAAGDESGFVDHILGADGSGATLGRAGHPGDEEKLFQNGREVFKFAVRI
                     LPEMVEKLLARNGLSAEEVQYVIPHQANARIIRAAIRKMDVPEDRVVVNLDRFGNTSA
                     ASIPLSYPDIFETLEPGKYVVTVGFGFGLTWAANLYRI"
     gene            complement(4046..>4945)
                     /gene="plsX"
                     /locus_tag="AC16CM_15160"
     CDS             complement(4046..>4945)
                     /gene="plsX"
                     /locus_tag="AC16CM_15160"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3141219.1"
                     /translation="PEVVNAELGKDPGSNISQRSSGDAVGMEEEPSVTLRANPDASVA
                     VAARMVRDGEADALFSAGNTGATVAAALLRMGRLEGCRRPAIATMLPFPSPVMLLDAG
                     ATISCRPHDLLNFAILGSVFAKRYFGLEEQARVGLVNVGEEPSKGDDLTKEAYRLIDD
                     SDVRFVGNVEGRDIGRGVADVLVTDGFTGNVVLKTAEGVAREILLMVKDAVTGGLLRN
                     LAAGILRSRLVGVRDAVDPENYGGSFLLGVNGAVVIGHGNSRARGVENALLGITRAGD
                     GLTEELQRALYDDRARTSEGGTA"
BASE COUNT          837 a         1805 c         1423 g          880 t
ORIGIN      
        1 tcggctccgc ggctgactgc gtgctcttcg ctctccagta tcactgcccc cgcgccctcg
       61 ctcatcacga agccgtcacg gccctcgtcg atcggacggc tcgcgcgggt ggggtcatcg
      121 tttcggcggc tcaccgctcg gatcgcgcag aagccggcca tggagagcgg cgtgatcggc
      181 gcctcagatg agccggcaat aaccacgtcg gcgtcgctgc gccggatcag ctctagcccc
      241 tcggctatgg cctcaccgcc agtggcgcac gccaagaccg ggcactgggt cggacccgtt
      301 gccccgagtg cgatcgccag ctgccccgcc gccatgttcg gtaccatcat ggttatgagg
      361 aaagggctca ccctgccggc accgcgctcg ttcaggaccc tgtactcacg ctcccaggtc
      421 gagatgcccc cgatgccgct acccatgact atggccaccc gctcgggtct ctcgcgcacg
      481 aggtcaacta ttcccgcgct atcggccgcc tgaaggcctg cggctatgcc gagctgagcg
      541 tagcggtcca tcctgcgggc ctgcttcttg ctgacaaagt cggtcgggtc gaagtcccag
      601 cactcgccgg ctatacggga cgggtaggga gaggggtcga agatcgtgat ctccccgatg
      661 ccgctcttcc cgtgaagggc ggcatcccag aagttctcag tccccgttcc gaggggagta
      721 accgcccccg tccccgttat gagcgcgcgc accctaccgc tggaggaggc ctccgtcacg
      781 ctgcctggtg ctccatcacg tagtcgacgg cttcctcgac cgtggtgatc ttctcggctt
      841 catcgtccgg gatctccatc ccgaactgct cctcgagctc catgatgagc tcgaccaggt
      901 ccagggagtc tgcctccaga tcctcccgga aagacgcgtc ggacgtgacg tctccctcgt
      961 ccacccccag ccggtcggcg gtgatctcct gaatcttgcc gagtacttct tcgcgatcca
     1021 attcgtctcc tctccaagac ttatgcgaat taccaagctt ctgcgatagc gcgtagtgta
     1081 aacccaaaac gctctactgc atagtcatgc cgccgtcgac ggccaccgtc tggcctgtta
     1141 cgtagcccgc gccttcgccg gccaggaacg caacgacctc agccacctcc gcagcctccc
     1201 cgaacctgcc gaccggaacc tgggccagaa tctggtcttt gatctcctct gacaggccgc
     1261 ccgtaagctc cgtctccacg tagcccggcg cgaccacgtt ggccgtgata ttccgctggg
     1321 caacctcccg cgccacgctt ttggtgaacc cgatgatgcc ggctttggag gcggcgtagt
     1381 ttgcctgccc cgcgttccca accagaccca cgacggatga tacgttgacg atccttcccc
     1441 acctggcccg gatcatcggc cgcaacaccg cccgcgtgca caggtaggtc cccttgaggt
     1501 tggtgcttat tacctcgtcg aactcctcct ctttcatcct catcatgagg ttgtcgcgcg
     1561 tgagcccggc gttgttgacg aggatatcga tgcgaccgaa ggcgtcgttt acacccttga
     1621 tgagcgcggc gacatcgtcc tgtgaagcga cgtcgccctt gaagatctcg caccgcacac
     1681 ctttggaccg cacctcttct gcagctgcct cggcggtttc gtcgttagag cggtacgaga
     1741 tagccacatc agcgccctcg ctggcgagac gcagggcgac ggcgcggcca atccccctcc
     1801 cgccacctgt aacgacggcc acacgcccct cctggatcac gggatctccc cccgctcgac
     1861 gaaggcgaac atgagctcgg cctcacagac ccgctcatcg ccgaccagcg cccgtccctt
     1921 gccccttccg accctgccct tgaggcggac tatctcaacc tctagggtca acacgtcgcc
     1981 ggggacgact tgcctgcgga acctaacgcc gtctatcccg gcgaagagcg cgagcttgtc
     2041 gccgtggtcc ccacccgaca tcacgccgac cgccccgacc tgcgccaacg cctcgataat
     2101 tagcacgccg ggcattaccg ggtactctgg aaaatggccc tggaagaagg gctcgttctg
     2161 ggtaacgttc ttgacgccgc tcgcccgcac ccctggttcg agctcatcga tcctgtccac
     2221 gagcaagaag gggtaccggt gcgggatgag ctgccggatc tccgccggac cgagcggcgc
     2281 cttcacgcga gcacctcccc caccgtcttg ctcgtaacgt cacgtcgctt gaaatcccgc
     2341 aacatccttg tcagcgttcc gtcctcccca gcctcgtaga tctcccgcac ccccaactcc
     2401 agcaaccgct cgaccacaga cacccagcgg accggcgaga gcatctggtt acgcaatgtg
     2461 ttcctcaccc cgttagcggt cgtcaaaagg gtcccctcgg tcgccccgat aatcgggatg
     2521 ccgggttccc caaactcgac cccatcgagc aatacgtcca tctccctggc cgcctcggcc
     2581 attagcgggg aatgaaacgc gccggcaaca cgcaatggta ccttgcggcc cttcaggcct
     2641 tccgtaacct cctccagcgc gtccagtctg ccggagatca cgacctgccg tggcgagttg
     2701 aaattggcgg cgaccacggc cccgccggag ccttcggccg cctcgaccac ggcatcgggg
     2761 tccgcgccga tcagggctac catcccccca ggggtcttcc gcgaagcctc gttcatgagg
     2821 cggtccctct cggcgacgag tcgcacgccg tcctcgaggc ccaggctacc ggcggcgtgg
     2881 gccgccgcgt actcccccag cgaatggcca gcaaccacgt cgccgtcgat gccggcgtcc
     2941 cgaagacggt agagcaggtc cacggaattg gcaaacacgg cgagctggta cggtacctcg
     3001 cggtcgccga ggtactctag aaccgcggcg cgcacggaac ctgaggtctc acctcccagg
     3061 ctcccctgtc ccgggaagac cacggcacgg ttcatctctc ccacctagat cctgtacagg
     3121 tttgcggccc acgtaaggcc gaacccgaat ccgacggtca ccacgtactt gcccggctcc
     3181 agcgtttcga agatatcggg gtaggagagc ggtatgctcg ccgccgaggt gttcccgaac
     3241 ctgtcgaggt tgacgacgac cctgtcctcc ggcacgtcca tcttgcgtat cgccgcgcgt
     3301 atgatgcgcg cgtttgcctg gtggggaatg acgtactgaa cctcctcggc cgacaacccg
     3361 ttgcgcgcga ggagtttctc taccatctcc ggcagtatcc tgaccgcgaa cttgaagacc
     3421 tcgcggccgt tctggaagag tttctcctcg tcccccgggt gaccggcccg cccgagcgtc
     3481 gccccgctac cgtcggcccc gaggatgtgg tccacgaagc cggactcatc tccggccgcg
     3541 tcgagcacca ccgcacctgc gccgtcgcca aacagcaccg ccgtcgagcg atcgtttgga
     3601 tcgactatag tgctcatcgc gtccgccccg atgagcagga tcctgctcgc cgcgccggct
     3661 cggatcatgg acgaggcaac ggaggcggcg taggagaaac cggcgcacgc ggcggccaga
     3721 tccatcgcgc ccgcacccgg taccccaacg gcttccccaa cgaggcaggc gacagagggc
     3781 atggactccg gggctgtgga ggtgccgcat ataacgaggt caaccgagcc accatcaacc
     3841 ccagcgtgct ccagggcctt ccgggacgcc tcgatggcga gggtggccgt gttttctccc
     3901 tccgcgacga agcggcgctg cttgatgcct gttcgggtgg agatccactc gtcagaggtg
     3961 tcgagaacgg tctcgaggtc agcgttggtg acgaccctgc tcccgagcgc cctgcccacc
     4021 ccgatgatct tgccggccgc gacgttcacg ccgtcccgcc ctcggaggtg cgcgccctgt
     4081 catcatagag cgcgcgctgc agctcttcgg tcaggccatc tccggcgcga gtgatgccga
     4141 gaagggcgtt ctcgacgccc ctggcgcgcg agtttccgtg cccgatcacg accgcgccgt
     4201 tcacgccgag caagaatgaa ccaccgtaat tttcggggtc tacggcatcc cgcacgccta
     4261 ccaggcgcga acgcagaatg ccggcggcca ggttccgcag aagaccgccc gtgaccgcgt
     4321 ccttcaccat aagcagaatc tcacgcgcca cgccctcggc cgtctttagc acgacgttcc
     4381 ccgtaaagcc gtcggtaacg agcacatccg ccacaccacg cccgatgtcc cttccctcca
     4441 cgttccccac gaaccgaacg tcggaatcgt ctatgagccg gtacgcttct ttggtgaggt
     4501 catccccttt gctgggctcc tcgccaacgt tgacgagccc gacacgcgcc tgctcttcca
     4561 acccgaagta ccgtttcgcg aacacgctcc ccagaatagc gaaattcaag aggtcgtgcg
     4621 gccggcagga gatcgtagcc cccgcgtcga gcagcatcac gggcgaagga aagggaagca
     4681 tggtggctat ggctggccgg cggcaccctt cgaggcgccc catccggagc agcgccgccg
     4741 caaccgtcgc cccggtgttg ccggcggaga atagcgcgtc ggcctcccca tcgcgtacca
     4801 tacgcgccgc cacggcgacg ctcgcatcag ggttggcccg cagcgtgacc gagggctcct
     4861 cttccatgcc cacggcgtct ccggaggacc gctgggaaat gttgcttcca gggtccttgc
     4921 cgagctcggc gtttacgacc tccgg
//
LOCUS       DBNGVD010000565         3761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1972613, whole genome shotgun sequence.
ACCESSION   DBNGVD010000565 DBNGVD010000000
VERSION     DBNGVD010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1972613"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16CM_15165"
     CDS             <1..609
                     /locus_tag="AC16CM_15165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3141220.1"
                     /translation="AVGSPSGFESTVTSGIVSATGREVPAEITGGQQESSLVDLVQTD
                     AAISPGNSGGALADRDGRVIGINVAYLPPQQTGAENIGFAIPSRTATTVADQIIENGR
                     AVHPYLGVYLSDLTSETARRFGSSVKTGALIEKVESGGPADTAGLRQGDVVTAAGNDQ
                     IQSSGDLLSALRNYQPGDNLTLSVLRDGQKETLQVELAESPQ"
     gene            complement(689..1582)
                     /locus_tag="AC16CM_15170"
     CDS             complement(689..1582)
                     /locus_tag="AC16CM_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3141221.1"
                     /translation="MTAGGESKRRGTPTVEAALLLTVFFFGTNFTAVKIVVDSVPPIL
                     FAATRFTFAGSLLLLFIPLLKGENRLRREDFLPVLGLGIVGITLTQTVFTVGVSLTTA
                     ANTALVYSTAPVWGMLLGFVLGLERPRLTGVLGIGLSLLGVGFIVYGGLDFAGTSLIG
                     DALILAAAVFWGSYTVLSLSLLRRYTPLALAAYAMTLGGLAAFPLAILVPGGFDVSSL
                     DGLTWGAAAYSTLFSSAFGFAAWGWGVSHVGANRVLIYQYLVTLTGVSTGIVLLGEDF
                     GFGQLVGAAVIIGGVYLARRP"
     gene            complement(1595..2653)
                     /locus_tag="AC16CM_15175"
     CDS             complement(1595..2653)
                     /locus_tag="AC16CM_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3141222.1"
                     /translation="MRDTSYDFLKRLLSTPGPSGYEEAAARVWREEAAKFADVRGDRM
                     GNSFATIGSGGSPKIMLAGHIDEIGVMVTHIEDSGLLRFTGVGGWDSQVLVGQRIRLL
                     TGGGEIVGVIGKKAIHVMEPEERKKVSEIKNLWIDIGAKDADEAKGLVRVGDVGVIDQ
                     DLVELPNGRIASRSVDNRMGAFVVLEALRLLFEEENIGAEVVAVACVQEEIGLYGARG
                     AAFGLDPDAAIAVDVTHATDTPGVSKNEHGDHELGSGPVLKRATNISPIVAEGLIQAA
                     ENENIPYTLEADSRSTGTDADAIQFARAGIATGLVSCPNRYMHSPNEVADLDDIENCA
                     RLISAYIKTLGEGTDFVR"
     gene            complement(2709..3413)
                     /locus_tag="AC16CM_15180"
     CDS             complement(2709..3413)
                     /locus_tag="AC16CM_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HIR3141223.1"
                     /translation="MIVRERPDSFLLFGQHEHGLMAGEFARRWGGGAGRGPEPFGSTV
                     YAVANHDAAWRELDGEVLWNEEKDRPYSFMDYPLPLKLPAQKRGIDQVEAHDPYAGCL
                     CSMHYARFLLGAERREEVAFREGELERQRGLEEGMSEEERENLERNFQFLRVCDGLSL
                     FLCLNEPGGGEDPPPYPGGFAFDGTRFRLVWEDDRTLSLDPNPFSEAFGVQVPYRIVG
                     KDRRYLGSDILELGIR"
BASE COUNT          709 a         1264 c         1176 g          612 t
ORIGIN      
        1 gctgtaggga gcccctccgg tttcgagtcg accgttacct ccggtatcgt cagcgcgacg
       61 ggccgcgagg tgccggccga gatcaccggc ggccagcagg agagctcgct ggtcgacctg
      121 gttcagaccg atgctgccat ctcccccggc aactcgggcg gcgccctcgc cgaccgcgac
      181 ggtcgggtca tcggcatcaa cgtggcatat ctgccaccac aacagacagg ggcagagaac
      241 atcggcttcg ccatcccatc gcgtacggcc accaccgtgg ctgaccagat catcgaaaac
      301 ggcagggccg tccaccccta tcttggagtc tacctctccg acctgacctc ggagacggct
      361 agaaggttcg gtagctccgt caagacgggc gcccttatcg aaaaggtcga gtccggcggt
      421 cccgccgaca cggccggcct ccgtcagggc gacgtggtga ccgccgccgg aaacgaccag
      481 atccagagtt cgggagacct tctctcggcc ctccgcaatt accagccagg ggacaacctg
      541 acgctgtcgg tcctgaggga cggccagaag gagaccctgc aggtagagct ggccgaaagc
      601 ccccagtagt actgatctgg caaagtcacc gaaggcgagg gcgtcccatg gacggaacgc
      661 cctcctgccg tctcgctcga gaacggggtt aggggcgtct cgccaggtag acgccaccga
      721 tgatgaccgc ggcaccaacg agttgtccaa agccgaaatc ttccccgagc aggacgatac
      781 ccgtactcac gcccgtcagg gtgacgaggt actggtagat caggacgcgg ttggccccga
      841 cgtgtgagac accccacccc caagcggcga acccgaaggc cgacgagaac agcgtcgagt
      901 aggcagccgc cccccacgtg agaccatcga gggagctgac gtcgaagcct cctggtacca
      961 ggatggcgag cgggaaagcg gccagaccgc caagggtcat ggcgtaggcg gcgagggcga
     1021 ggggcgtgta gcgcctcagc agcgacagcg acagcacggt gtaggagccc cagaaaacgg
     1081 cggcggcgag tatgagggca tccccgatga ggctcgtgcc ggcgaaatcc aggccaccgt
     1141 acacgataaa cccgaccccc aacagcgaga ggcctatgcc gaggacgccc gtcagcctcg
     1201 gcctttccag cccgagcacg aaccccagca gcatgcccca gacgggggcc gtcgagtaga
     1261 cgagggcggt gttggcggcc gtcgtcaggc tgacgcccac ggtgaatacc gtctgggtca
     1321 gggttatgcc gactatgcca aggccaagca cgggcaagaa atcctcgcgg cgtagcctgt
     1381 tctcaccctt cagtaaaggt atgaagagga gaagcaggga gcccgcgaag gtgaagcgcg
     1441 ttgcggcgaa caggatcggc ggaacgctgt cgaccacgat cttgaccgcg gtgaagttgg
     1501 tcccgaagaa gaagaccgta agaagcagcg cggcctcgac cgtcggcgtg ccgcgccgct
     1561 tcgattcccc tccagcggtc accgggggat caggctaccg gacgaaatcc gtgccctcgc
     1621 cgagggtctt gatgtaggcg gagatcaacc tggcgcagtt ctcgatgtcg tcgaggtcgg
     1681 cgacctcgtt gggcgagtgc atgtagcggt tggggcacga cacgagcccg gtggctatgc
     1741 cggcgcgcgc aaactgtatg gcgtcggcgt ccgtacccgt agagcgcgaa tcggcctcga
     1801 gcgtataggg gatgttctcg ttctctgctg cctggatcag tccctcagcc acgatcgggg
     1861 agatgttggt cgcccgcttg aggacgggac cgctgcccag ttcgtggtcc ccgtgctcgt
     1921 tcttggagac gccaggggta tccgtggcgt gggtcacgtc gacggcgatg gcggcgtcgg
     1981 ggtcgagccc gaaggcggcc ccccgcgccc cgtacagacc tatctcctcc tgcacgcagg
     2041 ccaccgccac cacctcggcg ccgatgttct cctcctcgaa caggagcctg agggcctcga
     2101 gcaccacgaa ggcgcccatg cggttgtcca cggagcgaga agctatgcgc ccgttcggga
     2161 gctccaccag gtcctgatcg atgacgccga cgtcgccgac gcgcaccaag cccttcgcct
     2221 catcagcgtc tttggcgccg atgtcgatcc agaggttctt tatctcggag accttcttgc
     2281 gctcctcggg ctccatgacg tggatggcct tcttgccgat cacgccgact atctcgccgc
     2341 cgccggtgag cagcctgatg cgctgcccca cgaggacctg cgaatcccaa ccacccacgc
     2401 cggtaaagcg gagcaggccg ctatcctcga tgtgggtgac catcacaccg atctcgtcga
     2461 tgtgccccgc gagcatgatc ttcggcgagc cgcccgagcc tatggtggcg aaggagttgc
     2521 ccatcctgtc gccgcgcaca tcggcgaact tcgcggcctc ttcccgccag accctggcgg
     2581 cggcctcctc gtaaccgctc ggccccgggg tcgagagcag cctcttcaaa aaatcgtacg
     2641 atgtatcccg catgctcgtc ctttctaaga cgaaagtgtg ctcgaagaag cgcccgtcgg
     2701 cgccccgttc accttatacc gagctcgagt atatcgctcc cgagatatcg ccggtccttg
     2761 ccgacgatcc tgtagggaac ctgtaccccg aacgcctcgg aaaaggggtt gggatccagg
     2821 ctcaaggtcc ggtcgtcctc ccacacgagg cggaatcgtg tcccgtcgaa agcgaagccc
     2881 cccggatacg gaggcgggtc ctccccaccg cctggctcgt tgaggcagag gaacagcgag
     2941 agcccgtcgc acacccgcaa gaactggaag ttccgctcca ggttctctcg ctcctcctcc
     3001 gacatcccct cctcgagccc gcgctggcgc tcgagctcgc cttcacgaaa cgcgacctct
     3061 tcccgacgct cggcgccgag caagaaccgg gcgtaatgca tgctgcaaag acaccccgca
     3121 tagggatcgt gggcctccac ctggtctatt ccccgcttct gcgccggcag tttcagcggt
     3181 agagggtagt ccataaaaga atagggccta tccttctcct cgttccacag aacctctccg
     3241 tcgagctcgc gccaggcggc gtcgtggttg gcaacggcat agaccgtgga cccgaacggt
     3301 tccggccccc taccagcccc cccaccccac cgtcgcgcga actcacctgc catgagaccg
     3361 tgctcgtgct gcccgaaaag aagaaaggaa tcgggccgct cgcgaacgat catggggcca
     3421 agcttaccag cttggcccca cgctgcctgc ggcagctcgt cagcacgctc cggcctgtcg
     3481 gcctccgttt gtcagcacgc cagcttcgct tcgctacgcc ggcttgtcgg caacgcttcg
     3541 gcttcgcctc gctttcggcc agtcgactct attcgtaacg gcggcttcgg catagggctt
     3601 tatcgagcat cgaatcttta gagcggtaag gtgcaggccg gaacgggatc tagctgacag
     3661 cgagccctgt aagggcgaag cgtgctgaca agcgagggcg ccagcccgag cgtgctgaca
     3721 agctggcgta gcggagcgaa gccggcgtgc tgacaagcgt a
//
LOCUS       DBNGVD010000566         4954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_333047, whole genome shotgun sequence.
ACCESSION   DBNGVD010000566 DBNGVD010000000
VERSION     DBNGVD010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_333047"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC16CM_15185"
     CDS             complement(<1..872)
                     /locus_tag="AC16CM_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3141224.1"
                     /translation="MPLTDSFHRGASSPNEVFGPDGEPYSHYRDVLTEMERMGPREWG
                     RRAAGARAWLVERQRELGMEPDEGVHPTDFVPRIVPAADWDLLERGLAQRMRAINQWL
                     VRLEAGRDEVVPEEVIRSSSLFDASVPMRFGSVPNRQMGFDVVAEARAGGWEYLIIED
                     NAKMPVGIEPMSLLRTMTPDVLPESYQTLGVKPLDGLMDLFGEVVRAASPEPDPTVAV
                     LSTGPEDQYYIDHRIFAAELGAILAEHDEVEIDARGRLVHKGTGRRIDVVYERIETGR
                     IFDYVPGLVESHYRG"
     gene            complement(1012..1419)
                     /locus_tag="AC16CM_15190"
     CDS             complement(1012..1419)
                     /locus_tag="AC16CM_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141225.1"
                     /translation="MRVPKRISKFFRDPPRTLFEDLEDSKAEAPVRGPLFPNHLFYGL
                     MTLFCLSLMLTADWFGPLLLNSDGVFGRIGPLNTVRVTAVLMTGIAIPSALLMFHYKR
                     LYSYTSDENVFFAVTFALSFLLGSPCVLVGIFG"
     gene            complement(1460..2266)
                     /locus_tag="AC16CM_15195"
     CDS             complement(1460..2266)
                     /locus_tag="AC16CM_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3141226.1"
                     /translation="MSDAPSPVSGPTDLSGRVALVTGVGRRRGIGSAVCRTLASRGAD
                     VVFSYWRAYDSGSPWASEEDEPQALLGELRASGVRAEGVEVDLSPPDSAWRLLDAAER
                     AIGRPSILVNVAAYSRRDGFEELDAETLDAHYAVNVRAMALLSVGFARRYPGGPGGRI
                     VNFSSGQSLGPMPQELAYVSTKGAIEAFTRTLAAEVGHKGITVNAVNPGPTDTGWISN
                     ELEQDLIGRFPLGRIGQPEDAARLVAFLASDEAAWITGQIINSEGGFVRS"
     gene            2550..4019
                     /locus_tag="AC16CM_15200"
     CDS             2550..4019
                     /locus_tag="AC16CM_15200"
                     /inference="COORDINATES: protein motif:HMM:NF013636.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3141227.1"
                     /translation="MIRTVFERTRFVGDPRGGLRLPVVRWLILMVLVCFLASLAVAAS
                     ASAATSFTNTSPITIPSSGNASPYPSEINVGGLTGPITDISVTLHRFGHKFPSEVDIL
                     LVSPGGKGVILMSDACSNAGNIEDFTWTFSQSAPGPMSGDCSAFTYRPTNVSDGDFWP
                     QAPAGPYSTSLDAFDNEAANGSWKLFVVDDTGDDGGDIEGGWSLSIDTGPVDLAIPGT
                     GTSGPASFYPATRTVSGQEGLISDLDVTIDGIWHQNPDDLDLLLVGPQGQKVILMSDA
                     CGSFGVRSFGWVWSDEAPAPMPDGDGTNVCSARFQRPTDYEPGDAWPTPAPPGPYSTS
                     LSAFDRTDPNGTWQLFVNDDSTGNTGFFTNRFTLGITTDTTPPTVVSVSPANDARGVR
                     RVTNVTATFSEAMKPGSINRNTFELFKAGTSTRIGATVSYDGMGGRALLDPNSNLKPG
                     TRYKAVVTTGAKDLSGNGLDQNPNVTGSQQKSWSFTTKN"
     gene            complement(4146..>4954)
                     /locus_tag="AC16CM_15205"
     CDS             complement(4146..>4954)
                     /locus_tag="AC16CM_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIR3141228.1"
                     /translation="ALAMAVAWLLGGGPALVSEAGVTALFTVMLDPSTSGWSPERFMD
                     ALLGAGVALGVRAVFPSDPRHREERAAHTIFDDLTGALRETATALRAGNLEMAEHALQ
                     KARETDGHMHELKEALEGGYDTVRLSPPRRHALGQLALYSTAADQLELAVRNVRVLAR
                     ATASTVREGGTVPEALSEPIFDLAYAVESLAAYIEEPEHPMDTRGFALKAAEGATAVL
                     QESNDLSTSMLVGQIRSTAVDLLRASGMDRTEALDTFYDAASRAGQRPAS"
BASE COUNT          878 a         1634 c         1581 g          861 t
ORIGIN      
        1 cccctgtagt gagactcgac caggccaggc acgtaatcga agatcctgcc ggtctcgatg
       61 cgctcgtaga ccacgtcgat cctgcggccg gttcccttgt ggacgagcct tccccgggca
      121 tctatctcga cctcgtcgtg ttcggccagt atggccccca gctcggcggc gaagatcctg
      181 tggtcgatgt agtactggtc ttctgggccg gtggagagga ccgctactgt cgggtcgggc
      241 tccggggaag cggcccgtac gacctcgccg aacaggtcca tgagaccgtc gagcggcttt
      301 acacccagcg tctgatagga ctcgggcagc acgtcggggg tcatggtccg gagcaggctc
      361 atcggctcga tgccaaccgg catcttggcg ttatcctcga tgatcaggta ctcccacccg
      421 ccagcacgag cctcggcgac gacgtcgaag cccatctgcc ggtttggaac gctcccgaac
      481 cgcatcggga ctgaagcgtc gaatagcgag ctgctcctga tgacctcctc tggcacgacc
      541 tcgtcccgtc cggcctccag gcgcaccagc cactggttta tggcgcgcat ccgctgcgcc
      601 aggccccgct ccagcaggtc ccagtccgcg gccggcacga tgcgtggcac gaagtctgtg
      661 ggatggaccc cctcgtccgg ctccatgccc agctcacgct gcctctcgac cagccacgcg
      721 cgggcgccag ccgccctcct cccccactcc cgcggcccca ttctctccat ctccgtgagc
      781 acgtcacggt agtgcgaata gggctctcca tctgggccga agacctcgtt gggcgaggac
      841 gcaccacgat ggaagctgtc agttaaaggc attcaaccgt cagcttctcg tcggaggacg
      901 cttcttgcgt tgaataatga gatgtcatcc ggcagatagt agcgttttcc tggggccgcc
      961 gctgccttcc cccttgctga aagctagaag ctgccggctg atagctggcg tttacccgaa
     1021 tatacccacc agaacgcatg gggagccgag cagaaacgag agggcgaagg ttacggcgaa
     1081 gaagacgttc tcgtccgagg tgtacgaata gaggcgtttg tagtggaaca tcaggagggc
     1141 cgaggggatg gctatgccgg tcatcaggac ggccgttacg cgcacggtgt tgagggggcc
     1201 gatcctcccg aacacgccat ccgagttgag gaggagcggc ccgaaccagt ctgcggtcag
     1261 catgagggag aggcagaaga gggtcataag cccgtagaac aggtggttgg ggaacagcgg
     1321 tccccttacg ggcgcctctg ccttcgagtc ctccaggtct tcgaacaacg ttctgggtgg
     1381 atcgcggaag aatttggaga ttcttttcgg aaccctcatg aggtgcgtaa tggtatcgca
     1441 aggaggcaga ttatgtagct caggaacgga cgaagccgcc ctcggagttg atgatctggc
     1501 cggtgatcca ggccgcctcg tcggaggcga ggaaggccac gagcctcgcc gcgtcctctg
     1561 gctgcccgat cctacccagg gggaatctgc ctatgaggtc ctgttctagc tcgttggaga
     1621 tccaacccgt atcggtggga ccggggttga cggcgttgac cgtgatgccc ttgtgcccca
     1681 cctcggcggc gagcgtcctg gtgaaggcct cgattgcacc cttggtcgag acgtaggcga
     1741 gctcctgggg catgggcccg agcgactggc cagagctgaa gttgacgatc ctgcccccgg
     1801 gtccacccgg gtacctcctg gcaaacccaa cgctcaagag cgccatcgcc cgcacgttga
     1861 ccgcgtagtg ggcgtcgagg gtctcagcgt cgagctcctc gaagccgtcg cgacgcgagt
     1921 aggccgccac gttcaccagg atggagggtc tccctatagc tctctcggcc gcgtcgagca
     1981 ggcgccacgc ggaatcgggc ggggagagat ccacttccac cccctcggcc cggacaccgg
     2041 aggcccgcaa ctcgccgagc agcgcctgcg gctcgtcttc ctccgacgcc cacggtgacc
     2101 cgctgtcgta agccctccag taagagaaga caacgtcggc gccgcgtgag gcgagcgtcc
     2161 ggcatacggc cgaccctatg cccctgcgac gtccgacgcc cgtcacgagc gccacacgcc
     2221 cgctcagatc cgtcgggccg gagacgggag atggtgcatc agacatgggc tggtcctcct
     2281 gttagtggat ctgacttccg agcgtacttt gcgcccggga accctgtcgt gcaaggggtc
     2341 ggctttcggc ggtcagcttt cagcggtcag caagagtagc tggtcctctc ttgccgcgaa
     2401 gataggcaat gttgccgata ccggcttcgc ttccgtggtc tacggtggtt ttgttacatg
     2461 gatatgccag gagaagggag tccatgccag aaaccggctt gccgctactt agctgacgat
     2521 gagagacatc ccgggaaagg agcgaacgga tgatcaggac ggtcttcgag agaacgaggt
     2581 ttgtgggaga tccccgagga ggcttgcgcc taccggtcgt gcgctggctg atcctgatgg
     2641 ttctcgtctg ctttctcgcc tcgctagcgg tggctgcgtc tgcctcggcg gccacgtcgt
     2701 tcacgaacac ctccccgatc acgatcccca gctcgggcaa tgcgtcgccc tatccctcgg
     2761 agatcaacgt cggtggcctg accggcccga tcactgatat cagcgtcacc cttcaccgct
     2821 tcgggcacaa atttccttct gaggtcgaca tcctgctcgt ctcccccggc ggcaagggcg
     2881 tcatcctcat gtctgacgcg tgcagcaacg caggcaacat cgaagacttt acctggacgt
     2941 tttcccagtc cgccccgggc ccaatgtcgg gggattgctc ggcgttcacc tacaggccaa
     3001 ccaacgtgtc cgacggggac ttctggcctc aggcgcctgc gggaccctac tcgacgagcc
     3061 tcgacgcgtt cgacaacgag gccgcgaacg gcagctggaa gcttttcgtg gtcgacgaca
     3121 cgggggacga cggcggcgat atcgagggcg gttggtcgct ctcgatcgat acgggtcccg
     3181 tcgacctcgc gatccctggc acgggtacct caggccccgc cagcttctac ccggccaccc
     3241 gcacggtctc aggtcaggag ggcctgatct ccgatcttga cgtgaccatc gacggcatct
     3301 ggcaccagaa ccccgacgat ctcgacctgc tgctggtcgg gccgcagggc cagaaggtga
     3361 tcctgatgtc cgacgcctgc ggctcgttcg gcgtgagatc cttcgggtgg gtgtggagcg
     3421 atgaggctcc ggcccccatg ccagacgggg acggcaccaa cgtctgcagc gccaggttcc
     3481 agaggccgac cgactacgag ccgggtgacg cctggcccac gccagcccca ccgggcccgt
     3541 actccaccag cctctccgcc ttcgaccgca ccgaccccaa cggcacctgg cagctcttcg
     3601 tcaacgacga ctccaccggc aacacggggt tcttcaccaa ccgtttcacc ctggggatca
     3661 ccacggacac gacccctcca acggtggtga gcgtaagccc cgccaacgac gccagggggg
     3721 tcaggcgcgt taccaacgtg accgccacct tctcggaggc tatgaagccg ggctccatca
     3781 acagaaacac cttcgagctc ttcaaggcgg gcacgagcac ccgaataggg gccacggtaa
     3841 gctacgatgg gatgggcggt agggcgcttc tcgaccccaa ctccaacctg aaaccaggga
     3901 ccaggtacaa ggcggtcgtg acgaccgggg cgaaagacct ctccggcaac ggtctggacc
     3961 agaacccgaa cgtcacggga agccagcaga agtcctggtc tttcacgaca aagaactagg
     4021 gcggttcttc gggccgagtg aacccgggag ggtcagggca tcaggcatca ggcttcagga
     4081 aaagtcctga tgcctgatgc cttcctgcgg agcgggccat ccctcccgag agccgccctc
     4141 ggcggttaag atgcgggccg ctggccggcg cggcttgcgg cgtcatagaa ggtgtcgagg
     4201 gcctctgtac ggtccatgcc agaagcccgc aggagatcca cggcggtcga ccggatctgg
     4261 ccaaccagca tgctggtgct gaggtcgttg ctctcctgga gcacggcggt tgccccttcc
     4321 gcggccttca gggcaaaccc gcgggtatcc atcggatgct ccggctcctc tatataggcc
     4381 gcaagggact cgacggcgta ggcgaggtcg aagatgggct cggagagcgc ctcgggaacg
     4441 gttcctccct cgcgcaccgt ggacgcggtc gcgcgcgcga gcacccgtac gttgcggacc
     4501 gcgagctcca actggtcggc ggccgtcgag tacagggcga gttggcctag ggcgtgcctg
     4561 cggggaggag agagccgcac ggtgtcgtag ccgccctcga gcgcctcctt gagctcgtgc
     4621 atgtgcccgt ccgtctcccg cgccttttgc agggcgtgct cggccatctc gaggttaccg
     4681 gcgcgaagcg cggtcgcggt ctcccgcaag gcgcccgtga gatcgtcgaa gatggtgtgg
     4741 gcggcacgct cctcacggtg cctggggtcg ctcgggaaca cggcccgcac cccgagcgcc
     4801 acccccgctc ctagcagcgc gtccataaag cgctcgggcg accatcccga cgtggaggga
     4861 tcgagcatta ccgtgaagag cgccgtgacg ccagcctcgg agaccagcgc gggcccgccc
     4921 cctagcaacc aggccaccgc catcgccagc gcga
//
LOCUS       DBNGVD010000567        16870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2882744, whole genome shotgun sequence.
ACCESSION   DBNGVD010000567 DBNGVD010000000
VERSION     DBNGVD010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16870
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2882744"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="sppA"
                     /locus_tag="AC16CM_15210"
     CDS             <1..702
                     /gene="sppA"
                     /locus_tag="AC16CM_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3141229.1"
                     /translation="DPSVAAVVLEINSPGGGVTASDEMHQSIVDFERNTGQPVVVSMQ
                     DVAASGGYYISTAADRIVANETTLTGSLGVIFQITNFAEAADKYGVKQVVIKSGKYKD
                     IGNAFREMTPEERDIFQSIVDESYAQFVDVISEGRGIPEDRVREIADGRVYSGLQAER
                     LGLVDSFGGLDEASGIAGDLAGTSDTTVVRYVQQPTLTDTLLARFAPEEPQAEQILDA
                     AGLTLDPKPYYLYRP"
     gene            complement(993..2027)
                     /locus_tag="AC16CM_15215"
     CDS             complement(993..2027)
                     /locus_tag="AC16CM_15215"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR3141230.1"
                     /translation="MAQTSGSRESSGDDLAKNTSTYDVEAQAAFISQGSALGASSAQL
                     AAIRADVAEEEGLPDYSQVVDNDTGGRFSAPGWTQSSDGSGGGQWSHGGSYVSSGSGG
                     APARFKVKIPTSNDYTVYAWWAAFPDSSEAARFGIDTAAGRRWTSVDQSIDGGMWVKL
                     GTYAMSKGERTIQLSAGNTVADAVAVVRGDVTMPPDDGQSITTRESGTYSATSLRDPR
                     PRDVVRVARRWMFTPYRYATCTRSRMSCTCETKKTYKRFGHELGMGERQQWRYNDRKV
                     RRIYDRSSLRRGDEVFFKENGPYKGISHVAVFSGNGNVVHASVYYGKVVESKMKYING
                     YFAAKRYRLY"
     gene            complement(2308..3780)
                     /locus_tag="AC16CM_15220"
     CDS             complement(2308..3780)
                     /locus_tag="AC16CM_15220"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3141231.1"
                     /translation="MQAEEKSLQVRNLIGGTWEEKDGRDVEPVYNPATGEVVAETPLS
                     TREDVDRAVEQAKAAFPGWAATPVVERARILFRFKALLEEHFEELRDLVTLENGKDAR
                     DAGGEVRRGIEVVEFACGMPTLMMGETVREVARGIDNSSWRYPLGVVAAITPFNFPCM
                     VPLWTLPVAIGAGNAYILKPSERTPLCAVRLGELLSEAGLPDGVFNIVNGARETVDGI
                     LEHPGIEAVSFVGSQPVAEHVYRLGSAHGKRVQALAGAKNSMIVMPDAILDQAVPNIV
                     SSAYGNAGERCLAGSVLVAVGDVGDEIVERLKQAANSMKVGPGSEEGSELTPVIRDSH
                     RDKVRSYVDLGEEEGAEIVLDGREAPLEDGFFMGPTLLDRVTGEMRVGREEIFGPVLS
                     VVRVDSLEEAVEFTNGSPFGNACSIYTENGAAVRYWRENVDAGMLGVNVGVAAPMAFF
                     PFNGIKNSFYGDLHATGKDGVRFFTENKVEITRYLSDPAG"
     gene            complement(3770..4330)
                     /locus_tag="AC16CM_15225"
     CDS             complement(3770..4330)
                     /locus_tag="AC16CM_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141232.1"
                     /translation="MSEGGPEAARRLTEAFGEPYREVVRNAVDAQQRNVQLAQGWVDS
                     VTGLLESQAEANRALTRAMESHTRAVEEAIKSQERTSRALAESLESYKEVIERNTALQ
                     ENSTKLVQGFFGDVTSELRDGMQGSQEVARSLMEGSERQLEAFQAMLSEATRSYADLM
                     NAPFDLYRKNLEAFGKQGKQGGQDAG"
     gene            4665..4933
                     /locus_tag="AC16CM_15230"
                     /pseudo
     CDS             4665..4933
                     /locus_tag="AC16CM_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720792.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            complement(5007..5573)
                     /locus_tag="AC16CM_15235"
     CDS             complement(5007..5573)
                     /locus_tag="AC16CM_15235"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR3141233.1"
                     /translation="MDREIGPDPFAALKDGSEEYHVYDRHYEKIGRVDDVFIDERDAV
                     SYIGVKMGFFGTNSTLVPVELVRVNDIRRLVEVDEEAETIKHAPHFGHDEAVLPELED
                     RVRTYFGLEPLLPPGPAETYPPEDVTRFGPDARVDTEPGERVEEQDLLTGPPLVDPVD
                     PVPVDPVPVDRWNREAGKGGVVYHRRRV"
     gene            complement(5681..6205)
                     /locus_tag="AC16CM_15240"
     CDS             complement(5681..6205)
                     /locus_tag="AC16CM_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141234.1"
                     /translation="MSTSIGMAGEVRVPVGAPPGFVEHVYCYEFSTPHPPGRVWAWLN
                     DPATFTEGQIWPFHVEFIDGGFEPGVLNAHQGPFLNLAGAIGEVRGFERGVAYRDLKY
                     FYGSYAISPRLVRPTRLQFWAEESDGGTLVRLRLDSLVHRWFGRTWGRSQSIFWSRFP
                     RWMASELGDPAGRT"
     gene            complement(6292..7170)
                     /locus_tag="AC16CM_15245"
     CDS             complement(6292..7170)
                     /locus_tag="AC16CM_15245"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIR3141235.1"
                     /translation="MFLRIDKLQIELPRPAQADPESAGIVQELMGGKFGEMSTLMNYT
                     YQSFNMRGKSKIRPYYDLVANIAAEEMGHIELVANTINLLLDQTEASTDGVTPPLNFS
                     GMTGNPDHFLNFGLGTIPGGAAGKAWTGENVFNSGNLKLDLLHNFFLESGARMGKIRV
                     YEATQNPVAREMVGYLIVRGGVHQEAYAKALSDLSGVDVTKLLPVPEIDSNDFPDARK
                     YMDKGFHRILYRFSPDDYKEIGEIWNGLSAIDGTEREVEDGIPEGGEVPDLEPAPPLY
                     APNVSAEDIEEIAKRL"
     gene            complement(7464..9065)
                     /gene="pckA"
                     /locus_tag="AC16CM_15250"
     CDS             complement(7464..9065)
                     /gene="pckA"
                     /locus_tag="AC16CM_15250"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3141236.1"
                     /translation="MDLAVRKMTLERFGVENIGAVHENLPPARLVEAAVRRREGMVSG
                     SGALVVRTGKRTGRSPKDRFIIENAVTKNTVDWGTGNKAFSSNAFGALLDRAAAYIEN
                     LEEIYVVDAYAGADPRYSLNVQVVTEHAWQALFAHQLFRRPSREELDAFEPEWTVISV
                     PGLLTDPEDDGTESETFVGIDFERKVVLVCGTAYAGEIKKSIFTVLNFVLPTERGVFP
                     MHCSANVGKEGEVALFFGLSGTGKTTLSADPERCLIGDDEHGWSDTGVFNFEGGCYAK
                     TIDLSPEKEPQIWDAIRFGAVLENVVMDRRTREVDYTDGMLTENTRVAYPLEYIAGAV
                     PDGTGGHPSAVLFLTADAFGVLPPISALTPSQAAYYFLSGYTAKLAGTEADMETDVEA
                     TFSTCFGAPFLPLPATTYARMLSERLSTHGTRCYLINTGWSGGPYGVGNRIDIAATRE
                     MVRAVIGGKLDDAGTREDPFFGLNVPIEVPGVASEYLDPRGTWEDGEAYDEQATRLAD
                     LFRENFTRFESQVTEDVRNAGPHSS"
     gene            9272..11146
                     /locus_tag="AC16CM_15255"
     CDS             9272..11146
                     /locus_tag="AC16CM_15255"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3141237.1"
                     /translation="MTQQINQETAGVAQWHSLGQQLRVDSIRSSSGAGSGHPTSSMSA
                     ADLLAVLMGKYLRYDFDEPENPNNDHLIFSKGHASPLLYSVYKAAGAISDEELLTFRK
                     FHSRLQGHPTPQIPWVDVATGSLGQGLPIGVGVALAGKYLDKLPYHVWVLCGDSEMAE
                     GSMWEAFQHASYYKLGNLTAILDMNRLGQTRETMNGWHGDRYAARAEAFGWNAIQIDG
                     HAPDEIDGAYAEALEETDRPTLIVARTKKGRGVSFLEDVDGMHGKPVPGDDVQKALDE
                     LGDHDDLLVNVRKPDPGQGENFTPGLTGELELPTWEVGDEEATRGAYGATLKALGGAR
                     EDVVALDGEVSNSTKSEEFAEEYPDRYFEMFIAEQQMVSAAVGMSVRHRVPFASSFAA
                     FLSRAYDFIRMAAISRANIRLSGSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLQPC
                     DANQTARLVAEMADKDGIVYLRTLRPKTPVIYDASEGFPIGGSKTVRSSDEDAVTVVA
                     SGITLHEATKAADELQESGIAVRVIDAYSIKPIDGDTIRDAARATGGKVVVVEDHWPE
                     GGLGEAVLSSLSGRLDDLHFEHLAVEIMPGSGSSAELMDAAGISANHIVDAARKLANG
                     "
     gene            11378..14272
                     /locus_tag="AC16CM_15260"
     CDS             11378..14272
                     /locus_tag="AC16CM_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3141238.1"
                     /translation="MRTTISLLGAPHVEHDGVPVEVDTRKATALAAYLAVTRRGHTRD
                     ALAGLLWPEYGQARARAALRRTLSSLGKAREEGWLIADRESVDLDHDAIRVDVVEFEG
                     LLAACELHGHPRSEVCAECLPPLTEAVGLYRGDFMAGFGLRDSVAFDDWQFFQAESLR
                     RELGSALERLARGRGALGEWEAAVAHARRWLSLDVLHEPAHRMLIALYAFSDQRAAAL
                     RQYRECVRILDRELGVAPLEETTLLYRAIQENDLPPRPTLSEPMPAMGREAPPAKTTV
                     TVPSSPENPLVGRDPEWVALLGAYASIGEGGRIVVIEGEAGIGKTRLAEEFVAHAGEG
                     GATTVVARCYAGEKNLAYGPFIEGLSAALGRESGDRLADLPASSLQEAARLLPGLAPD
                     SSPAPPLDTPGARTRFFAEVIRVLQAILDGPPPGVLFLDDLHWADDASLGLLNYLIRR
                     LDDKPFCVLLTWRTEDVPEKHRLRELLAEARRSGTVTVLSLERLSPRFVEELVGNTVP
                     DAAGLGPRLSEETEGLPLFLTEYLAAVEKGELDPGDEAWTLPGGVQDLLRARLGTVDE
                     ASGQILATAAVVGRSFDFDTVRAASGRGEEETLDALEELISRGLIREVGGAGATPSYD
                     FDHDKLRTLVYEETSLARKRLLHRRTAAALAGGARGREASSLAGQIARHHRLAGQDLE
                     AARYYLLAGDYARSLHANSEALSHYEEALALGYPEAAALHESIGDLRTRTGEYGAALA
                     SYEAAASSAGPGDLAELEHKIGNVHARLGERDLARLHYESAVNEFSQTDSEAELARLY
                     ADWSLLAHGQDQPEEAAALARQALALAEITGDTRALAQAHNMLGILAGKAGDQEAALS
                     HLAKSLDLAEALGDSDARVAALNNLALAREGGGEPEEALKLAEAALKLCETSGDRHRE
                     AALHNNLADLFHATGREVESMEHLKRAVEIFAEIGERGELQPEVWKLVEW"
     gene            complement(14290..14763)
                     /locus_tag="AC16CM_15265"
     CDS             complement(14290..14763)
                     /locus_tag="AC16CM_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141239.1"
                     /translation="MWPEITTHLYEFESAVLSGVDEDGYPFSVRCRPYPDASGAEVLR
                     VWLPPGTPFRPGPASLLCHSHDENLWNLRGFLVRGVLFLDFGQWSFEPVRFVPGIGMG
                     GLPGMIRLFVRSRRNARSYLEKRGLARPSIPWGEINAAKREAFASSAGKGPEANG"
     gene            complement(14753..15277)
                     /locus_tag="AC16CM_15270"
     CDS             complement(14753..15277)
                     /locus_tag="AC16CM_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3141240.1"
                     /translation="MPRSSRIVAGLPPEVEDVFEEFRTCEMSTLARDGTPITWPTLPF
                     WYSDKKHFTITASIGFAQKALNVRRNPRVSLLFSNPTASGLEDPPAVLVQGDAVAPDE
                     IETSAVGFEEELRKVYPRQPASRFYGNDPLTRRLMDWYFMRLMIHVTPRRIMWWPGAD
                     FWREPFELRMDHVA"
     gene            complement(15794..>16870)
                     /locus_tag="AC16CM_15275"
     CDS             complement(15794..>16870)
                     /locus_tag="AC16CM_15275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR3141241.1"
                     /translation="GSRVSGEKFVVCAGGHARRMDFPGSEHALTHSDVWAMQSLPSSV
                     VVVGAAATGCQLASVFAAFGSRVRLLEVGPHILGGEDGVVSEGVAEVFSRRDIEIITG
                     IGGVDRIDREGKLLNLSYTQDGETRVLETEAVVLAVGWPGNVDRLNLAAAGIETRRGY
                     VTVDDCLRTSAPHVFAAGDLTGRMMLVQSANYEGSLAAENAVLGEDRGYRHAIVPHGG
                     FTDPEYGSVGLTERQARDEYGDGDCAVAVVPYTDLDRGVIDGRPDGSCKLIVSRSSRR
                     VLGAHIVGEQAVEVVQLAATAMGAGMRVEQLANLELAYPTFTAIIGLAARQLTRQLDL
                     IPTAPTWRAPERPVATEWEHNDMA"
BASE COUNT         2940 a         5616 c         5335 g         2979 t
ORIGIN      
        1 gacccgagcg tcgccgccgt ggtgctggag ataaactccc ccggcggggg ggtcacggcg
       61 agcgacgaga tgcaccagag catcgtggac ttcgagagga acacgggcca gcccgttgtg
      121 gtctccatgc aggacgtcgc ggcctcggga ggctactaca tctcgaccgc ggccgaccgc
      181 atagtcgcca acgaaacgac cctgacaggc tccctcggcg tgatattcca gatcaccaac
      241 ttcgcagagg ccgctgacaa gtacggcgtc aagcaggtgg tcatcaagag tggcaagtac
      301 aaggacatag ggaacgcttt cagggagatg acgccggaag agcgcgacat attccagtcg
      361 atcgtggacg agtcctacgc gcagttcgtg gacgtgatct ccgagggccg cggcatcccc
      421 gaggataggg tgcgcgagat cgccgacggg cgcgtctact cgggccttca ggccgagaga
      481 ctggggctcg tggattcctt cggcggcctc gacgaggctt caggtatcgc gggtgacctc
      541 gccggcacga gcgacactac cgtggtccgc tacgtgcagc aaccgacctt gaccgatacg
      601 ctcctcgcgc gcttcgcccc ggaggagcct caggccgagc agatcctgga cgccgcaggc
      661 ctgaccctgg atccgaaacc ctactacctc taccgaccct gaagtaccgg tgcgcgggac
      721 cgcaccgtcc cgcgggcggg ggatgcccga ggccatatcg gcccagacca ggccgtccgt
      781 acgggagagc cggaaggccc tcccatacag tcgctcccgc ggaagccgga acccacagcc
      841 tgaagtaacg cgtgtcccgc ctccgatgct gctgctatcc acccgtaccc gtcatgaacc
      901 cgagcccgta gagcggcagg ccggggcaac gaaaagggag gttcccagaa cgggaacctc
      961 ccctgttctc gggtccgaaa cccggagctt tgctagtaca acctgtagcg cttcgcggcg
     1021 aagtagccgt tgatgtactt catcttgctc tccactacct tgccgtagta gacggaggca
     1081 tggaccacgt tgccgttacc ggaaaagacc gcgacgtggg agataccctt gtaaggaccg
     1141 ttctccttga agaagacctc gtcacccctc ctcaggcttg acctgtcgta gatcctccgc
     1201 accttcctgt cgttgtacct ccactgctgc ctctcgccca tgccgagctc gtggccgaac
     1261 cgcttgtagg tcttcttggt ctcgcacgtg cagctcatcc tgcttcgcgt acaggtggcg
     1321 tacctgtagg gggtgaacat ccaacgcctc gccaccctga ccacgtcgcg cggtcgggga
     1381 tctctcagcg aggtcgcgga gtaggtgccc gattcgcggg tggttatgct ctgcccgtcg
     1441 tctggaggca tggtcacgtc gccgcggacc acggccaccg catcggccac ggtgttgccg
     1501 gccgaaagct ggattgtgcg ctcacccttg ctcatcgcgt aggtgccgag cttgacccac
     1561 atgccaccgt ctatgctctg gtccaccgaa gtccaccgcc tgccggcggc cgtgtcgatg
     1621 ccgaagcggg cggcctcgct gctgtctggg aaggcggccc accaggcgta cacggtgtag
     1681 tcgttgctgg tcgggatctt gaccttgaaa cgcgccggcg ccccgcccga acccgaactg
     1741 acgtaactgc cgccgtggct ccactgtccc ccgcccgagc catcactgct ctgcgtccat
     1801 cccggggcac taaaacgccc cccggtgtcg ttgtcaacga cctgtgagta atccgggagg
     1861 ccctcttctt ccgccacgtc ggcccgtatg gccgccagtt gcgctgaact cgcgccgagc
     1921 gcagaaccct gcgagatgaa ggcagcctgc gcctcgacat cgtaggtgct tgtgttcttc
     1981 gctagatcgt ctccggatga ctctcgcgac cccgacgtct gtgccaccgc cgtggtagca
     2041 accaaaccca aaccaataca gcccgcagag attattagga taagccgacg cacgtggccc
     2101 ccctcccaag ttcgtacttg cgtcggcgac cattgtaacc gtcctgtttc cgaaaagcac
     2161 tagttcgcta aagttttttt cagaaacttt gcagaaatgt tacggatagg taacaccgta
     2221 gggtgcaatg aggccttcag ccccattgca ccctgtcagc gttcagctat cagctgtcag
     2281 caagtaaagc tgatagctat cactactcta accggcgggg tcggagaggt agcgggtgat
     2341 ctctaccttg ttctcggtaa agaatcgaac gccgtccttg ccggtggcgt gcaggtcgcc
     2401 gtagaaggag ttcttgatcc cgttgaacgg gaagaacgcc atcggcgcgg ccaccccgac
     2461 gttgacgcct agcatcccgg catccacgtt ctcgcgccag tatctcacgg cggcgccgtt
     2521 ctcggtgtag atcgagcagg cgttgccgaa gggtgagccg ttggtgaact ccacggcctc
     2581 ctcgagggaa tcgacgcgca ccaccgagag gacggggccg aagatctcct cgcgtcccac
     2641 ccgcatctcg ccggttacgc ggtcgagaag cgtcgggccc atgaagaagc cgtcctcgag
     2701 cggcgcctct ctgccgtcga gcacgatctc ggcgccttcc tcctcgccga ggtcaacgta
     2761 ggagcgaacc ttatcccggt gagagtcccg gatcacgggc gtcagctccg agccttcttc
     2821 acttccggga ccgaccttca tggagttggc ggcctgcttg agcctttcga ctatctcgtc
     2881 ccccacatcc cccacagcca cgagaacgct gcctgcgagg caccgctcgc ccgcgttgcc
     2941 gtaggcggag gagacgatgt ttggcacggc ctgatcaaga atcgcatctg gcatcacgat
     3001 catagagttc ttggcgccgg caagcgcctg cacccgcttg ccgtgggcag agcccaacct
     3061 gtagacgtgc tcggccacgg gctgggagcc tacaaaggag acggcctcga taccgggatg
     3121 ctccagaatg ccgtccacgg tctccctggc cccgttgacg atgttgaaga ccccgtcggg
     3181 gaggccggcc tcggagagaa gctctcccag cctgacggcg cacaggggcg tcctctccga
     3241 gggcttgagg atgtaggcgt tgcccgcgcc tatggccacg gggagggtcc acaggggcac
     3301 catgcaggga aagttgaacg gggttatggc ggcgactacc ccgagcggat agcgccagga
     3361 ggagttatct atccccctcg ccacctccct gaccgtctcg cccatcatca gggtgggcat
     3421 cccgcaggcg aactccacga cctcgatgcc gcgcctgacc tcgccgccgg cgtccctggc
     3481 gtccttgccg ttctccaggg ttacgaggtc cctgagctcc tcgaagtgct cctccaggag
     3541 ggccttgaag cggaagagta tcctggccct ctccacgacg ggcgtcgcgg cccagccggg
     3601 aaacgccgcc ttagcctgct ctacggctcg gtctacgtcc tccctggtgg agaggggcgt
     3661 ctcggcgacg acctctcccg tggccgggtt gtagaccggt tcgacgtcac ggccgtcctt
     3721 ctcctcccag gttcccccga tgaggtttct tacctgtagg ctcttctctt cagcctgcat
     3781 cctgtcctcc ttgcttgccc tgcttgccga acgcttccag gttcttgcgg tagaggtcga
     3841 acggagcgtt catcaggtcg gcgtaggatc tggtcgcctc gctgagcatc gcctggaacg
     3901 cctccaactg ccgctccgag ccctccatca ggctgcgggc gacctcctgg ctgccctgca
     3961 tcccatccct cagctcggag gttacgtcgc cgaagaaacc ctggacgagc ttggtgctgt
     4021 tctcctgcag tgcggtgttg cgttcgatca cctctttgta cgactccagg ctctcggcga
     4081 gggcgcggct cgtgcgctcc tggctcttta tggcctcctc gacggccctg gtgtggctct
     4141 ccatcgctct ggtcagcgcc cggtttgcct cggcctggct ctcgagcagg cctgtaacgc
     4201 tatcgaccca gccctgggcg agctggacgt tcctttgctg ggcgtcgacg gcgttgcgga
     4261 ccacctccct gtagggctcg ccgaaagcct ccgtcaaccg tctcgccgcc tcgggtccac
     4321 cctcagacac tcgttcctcc tctcgcgggt atctggagac attctacccg tttgacggcg
     4381 caccacgtct acctccagac acgtcgaaag gcggcttggc cgcccggcgg cttctaccag
     4441 tcccaggcga actcgtcctt acgctccggg tgagtcccgg tgtctacaac cccgtgtccc
     4501 agggtacctt taatcggcgg cagaaggacg aagccgcccc gaccttcggg cgtgaccctg
     4561 gatttggtgc gagaacggct tcaggttggg ggcggcaggg ttctggccta gcctgacgga
     4621 gacaggaggt aagctccctt gaacggaacc atgctctacg ccttccaggc aagacggtgg
     4681 cggtcgtcgg cgacggtgcc gacgtgctcc acggcgtcga gattccgggc caggagtact
     4741 tcttctctca cgtacacctg aacggcggac ccgccccggt gagacgcttc ctgccgggct
     4801 gatcgttctg atctggaacc aggagatcta acccggcaag gtcttcggcc tgaccctgcc
     4861 gctggaggac gcggcggaag gccaacgcgc gatggacgag cggcgcgcga tcaggacgcc
     4921 tctgcgcccc tgaagggcag gctgcgatag acagatcaga ggacagggct acgtttcgac
     4981 gcgggtccgg tgcgagacgc gtatccctag actcttcggc gatggtagac gacgccgccc
     5041 ttgccggcct cccggttcca gcggtcgacg ggtacgggat ccacaggtac tgggtccacg
     5101 ggatctacga gcggcggacc ggtgagtagg tcctgctcct ccaccctctc gccaggctcg
     5161 gtatcaaccc tggcgtctgg accgaagcgg gtcacatctt cgggggggta tgtctcggca
     5221 ggccctggcg gcaggagagg ttcgaggccg aagtacgtgc ggaccctgtc ttccagttcg
     5281 ggcaagacgg cctcatcgtg gccgaagtgg ggcgcgtgtt tgatcgtctc agcctcctcg
     5341 tcgacctcga ccagccgcct gatatcgttg accctgacga gttcgaccgg gaccagggtg
     5401 gagttggtgc cgaagaaacc catctttacc ccgatgtagg aaacggcgtc cctctcgtcg
     5461 ataaagacgt cgtcaaccct tccgatcttc tcgtagtgcc tatcgtaaac gtggtactct
     5521 tcgctaccgt ccttgagcgc cgcgaagggg tcagggccga tctctctgtc cacaaccagt
     5581 cctttccaga agccgttacc gtcacgtcca aggagacctc gcacgggtag cgcgcactcc
     5641 aggacactct attgtattga cagcgcgggg cgtttttccg ttacgtccgg ccggcggggt
     5701 ccccgagctc cgaggccatc caccggggga aacggctcca gaagatgctc tgtgagcgtc
     5761 cccatgtccg tccgaaccag cggtgcacga ggctgtcgag ccgcagtcgg accagcgttc
     5821 ccccgtccga ctcctcggcc cagaactgca gcctggtcgg ccgcacgagg cgtgggctga
     5881 tggcatagct gccgtagaag tacttcaggt cccggtaggc gacgccccgc tcgaaccccc
     5941 gtacctcccc gatcgccccg gccaggttca agaacgggcc ttggtgcgcg ttcagaacgc
     6001 caggctcgaa gccgccgtcg atgaactcca cgtggaaggg ccagatctgg ccctccgtga
     6061 acgtcgccgg gtcgttcagc caggcccaca cccggcccgg cgggtgcggg gtcgagaact
     6121 cgtaacagta gacgtgctcc acgaaccccg gaggcgcgcc taccggcacc cgtacttccc
     6181 cagccatccc gatgctcgta ctcaagacat cctcgcttca tgcgcgtaga cagcgccgcg
     6241 aagtagtagt gcgcggcgcc gtctgcggtt ggttctctcc caaggtctgt tctagagccg
     6301 cttggctatc tcctctatgt cctcagcgct cacgttcggg gcgtagaggg gtggggccgg
     6361 ctcgaggtcg ggcacttcac cgccctccgg gattccgtcc tcgacctcgc gctccgtgcc
     6421 gtcgatggcc gagagaccgt tccagatctc ccctatctcc ttgtagtcgt cggggctgaa
     6481 gcggtacagg attcggtgga aacccttgtc catatacttc ctggcgtccg ggaaatcgtt
     6541 cgaatctatc tcaggcaccg gcagcaactt ggtcacgtcc acaccggaga ggtcgctcag
     6601 cgccttggcg taggcttcct gatgaacgcc gccgcgcacg atcaggtacc ccaccatctc
     6661 acgcgccacg gggttctggg tggcctcgta gacacgtatc ttgcccatcc tcgcgcccga
     6721 ctccaggaag aagttgtgca ggaggtcgag cttgaggttg cccgagttga acacgttctc
     6781 gcccgtccag gccttgccgg cggcgccgcc gggtatggtg ccgagcccga agttcaggaa
     6841 gtggtcgggg ttgcccgtca tcccggagaa gttcaggggc ggcgttacac cgtccgtgct
     6901 cgcctcggtc tggtccagga gcaggttgat ggtgttggcg accagctcga tgtggcccat
     6961 ctcctctgcg gcgatgttgg ccacgaggtc gtagtagggc cggatcttgc tctttcccct
     7021 catgttgaac gactggtagg tgtagttcat cagggtggac atctcgccga acttgccgcc
     7081 catcagctcc tgcacgatgc ctgctgactc ggggtcggct tgcgcaggcc ttggcagctc
     7141 tatctgtagc ttgtctatcc gaagaaacat gattctcccg ttctatggat gcttccgatc
     7201 cctatctcac ggtcttccgc tgccgatggg ccagccggtg cctcctcgcc atcgcatatc
     7261 ccggcccccg gtgcttagcc gggttgcggg cgcgcctaac cgtaccttgc tgacttcgtt
     7321 cagaagttaa agacgcaaac cgggatgaga ccgtaagcga tcgtacatcg gtgcgcttcg
     7381 gcagagcgca gcgggggtac ctttctgcgg gcagcgcgga gcgcgacgct acacgccatg
     7441 aacctgtagc ccgaaccctc ccgctacgac gagtgcggcc cggcgttcct cacgtcctcc
     7501 gtaacctgag actcgaacct ggtgaagttc tccctgaaga ggtcggcgag cctggtggcc
     7561 tgctcgtcgt aggcttcgcc gtcctcccag gtccctcggg ggtccagata ttcgctcgcg
     7621 accccgggca cctcgatggg aacgttcagg ccgaagaacg ggtcctccct ggtcccggcg
     7681 tcgtcgagct tgccacctat cacggctctt accatctcgc gggtggcggc gatatctatg
     7741 cggtttccca cgccgtacgg accgccggac cagccggtat ttatgaggta gcagcgggta
     7801 ccgtgcgtgc tcaacctttc ggagagcatc ctggcgtaag tggtcgccgg cagaggaagg
     7861 aacggggcgc cgaagcaggt ggagaacgtt gcctcgacgt cagtctccat gtcggcctcg
     7921 gtaccggcga gcttggcggt gtagcccgag aggaaatagt aggcggcctg cgagggcgtg
     7981 agggcgctta tggggggcag gacaccgaag gcgtcggccg tcaagaacag gacggcagag
     8041 gggtggccgc ccgtgccgtc cgggacggcg cccgctatgt actccagcgg gtatgcgacg
     8101 cgggtgttct ccgtcagcat gccgtcggtg taatccacct cgcgcgtgcg acggtccatc
     8161 accacgttct cgagcacagc cccgaagcgg atggcgtccc agatctgggg ttccttctcc
     8221 ggggacaggt ctatagtctt ggcgtaacag ccgccctcga agttgaagac cccggtatcg
     8281 gaccagccgt gctcgtcgtc cccgatcagg caccgctccg ggtccgccga cagagtggtt
     8341 ttacccgtgc cagagagccc gaagaagagc gccacttccc cctccttgcc aacgttggcc
     8401 gagcagtgca tgggaaatac gccgcgttcg gtcggcaaga cgaagttcag aacggtaaag
     8461 atgctcttct tgatctcgcc ggcataggcc gtgccacaga cgaggaccac cttgcgctcg
     8521 aagtctatgc ccacgaaggt ctcggactca gtgccgtcat cctccgggtc ggtgagcagc
     8581 ccggggaccg agatcacggt ccactcaggc tcgaaggcgt caagctcctc cctggaggga
     8641 cgcctgaaga gctggtgcgc gaacagggcc tgccaggcgt gctcggtcac cacctgcacg
     8701 ttgaggctgt agcgaggatc ggcgccagcg taggcgtcga ccacgtagat ctcttccagg
     8761 ttctcgatgt aggctgcggc cctgtccagc agcgccccga acgcgttgct cgagaaagcc
     8821 ttgttaccgg tgccccagtc caccgtattc ttcgtcacgg cgttctcgat gataaaacgg
     8881 tccttgggcg aacggccggt gcgtttgccc gtccttacga cgagggcccc agaaccggag
     8941 accatgccct ccctccgcct gacggccgcc tcgaccaggc gagctggcgg taggttctca
     9001 tgaacagcac caatgttctc taccccgaat cgctccagcg tcatctttcg aaccgccaga
     9061 tccatactct tcccttccac cgggtctcgt aatgtggatc caacccggta ctttttcctc
     9121 tcgccccagg aattctagca aagcacgcaa aaacccgttt gtatcaggga tgcacgctgg
     9181 gcagcgaacc cgtccagcgg tttctggctc gtccacgtgg ggtagtagga atagtgttta
     9241 ccggtatgcg attctgccga ggaggcaaac ggtgacgcaa cagataaacc aggagacggc
     9301 gggcgtggcg cagtggcact cgctcggcca acagctgagg gtcgacagca tacgctccag
     9361 ctcgggcgcg gggtcagggc atcccacctc ctccatgtcc gcggccgacc tcctggccgt
     9421 gctgatgggc aagtacctgc gttacgactt cgacgagcct gagaacccca acaacgacca
     9481 cctgatcttc tcgaaaggcc acgcctcgcc cctgctctac tccgtctaca aggcggccgg
     9541 cgccatctcc gacgaggagc tcctgacgtt ccgcaagttc cacagccgcc tgcaaggcca
     9601 tcccacgccc cagatcccct gggtggacgt ggcgacgggc tccctcggcc aggggctgcc
     9661 cataggcgtg ggcgtcgcac tggccggcaa gtacctggac aagctgccct accacgtctg
     9721 ggtgctctgc ggcgactccg agatggccga gggctcgatg tgggaggcct tccagcacgc
     9781 ctcctactac aagctcggca acctcaccgc catcctggat atgaaccgcc tcggccagac
     9841 ccgcgagacg atgaacggat ggcacggcga ccgctacgcc gcccgcgccg aggctttcgg
     9901 ctggaacgcc atacagatcg acggccacgc cccggatgag atagacgggg cctacgccga
     9961 agccctggaa gagaccgaca ggccgaccct gatcgtcgcc aggaccaaga agggccgcgg
    10021 ggtgtctttc ctggaggacg tagacggcat gcacggcaag cccgtccccg gggacgacgt
    10081 ccagaaggcc ctcgacgagt tgggcgacca cgacgacctt ctggtgaatg tacgcaagcc
    10141 cgatcccggt cagggcgaaa actttacccc gggcctgacc ggcgagctgg agcttccgac
    10201 ctgggaggtt ggagacgaag aagcgacccg cggcgcctac ggtgcgacgc tgaaggcgct
    10261 gggcggcgcc agggaagacg tggtggcact cgacggcgag gtgagcaact ccaccaagtc
    10321 cgaggagttc gccgaagaat accccgaccg ctacttcgag atgttcatcg ccgagcagca
    10381 gatggtctcg gcggccgtgg gaatgagcgt gcgtcacagg gttccgttcg cctccagctt
    10441 cgccgccttc ctctcccgcg cctacgactt catccggatg gccgcgatct cccgcgccaa
    10501 catccgcctc tccggctccc acgccggcgt ctccatcggc gaggatggcc cctcccagat
    10561 ggcgctggaa gacctggcga tgatgcgcgc tgtgcacggc tccaccgtgc ttcagccctg
    10621 cgacgccaac cagaccgcca ggctcgtcgc cgagatggcc gacaaagacg gcatcgtcta
    10681 cctgcggacg ctccgcccga agacgcccgt gatctacgac gcctcggaag gcttccccat
    10741 cggcggcagc aagactgtac gctcctcaga cgaggacgca gttaccgtcg tggcatcggg
    10801 tatcacgctc cacgaggcca cgaaagccgc ggacgagctg caggaaagcg gcatagcggt
    10861 acgcgtgatc gacgcctact ccatcaaacc gatagacggc gacacgatac gcgacgccgc
    10921 ccgggcgacg ggtggtaagg tggtcgtggt cgaggaccac tggccggaag gcggcctcgg
    10981 cgaagcggtc ctctcctcgc tctccggaag gttggacgac cttcacttcg agcacctcgc
    11041 cgtggagatc atgccgggct ccggtagctc cgcagagttg atggacgccg ccggcatcag
    11101 cgcaaaccac atagtcgacg ccgcacgcaa gctggcaaac ggctagggaa gcatctcccc
    11161 agacaacgag gcgccgggca ctggtgcccg gcgtctctct catgtccgga accccgtgtc
    11221 accgacggat cgtgtgacaa gtgtgtaggg gcggttcgtg gaccgcccaa gaatcacccg
    11281 accgttacga acatctctat gcatcgagta tcgttgcgcc gtcgtttcgg cgccggcata
    11341 atatcgtgga cgtagagtta cgggaggcac gaggttgttg cggacgacca tttcgttgct
    11401 cggcgcgccg cacgtcgagc atgacggtgt gcccgtcgag gtagacacca gaaaggcaac
    11461 cgcgctggcc gcgtacctgg ccgtcacgcg tcggggccac accagggacg cgctggctgg
    11521 cctgctctgg cccgagtacg gtcaggcccg agcccgcgcc gccctgcgca gaaccctctc
    11581 gtcgctgggg aaagcccgcg aggaaggctg gttgatcgcc gacagggaga gcgtggacct
    11641 cgaccacgac gccatccggg tcgacgtggt cgagttcgag ggcctcctgg ccgcgtgcga
    11701 gctgcacggg cacccgaggt ctgaggtatg cgccgagtgc ctgccgccgc ttaccgaggc
    11761 cgtcgggctg taccgcggcg acttcatggc cggtttcggg ttacgtgaca gcgtcgcctt
    11821 cgacgactgg cagttcttcc aggcagagag tttgcgccgc gagctcggga gtgcgctgga
    11881 gaggctcgcg cgtggccgcg gtgcccttgg cgagtgggag gcggccgtgg cccacgccag
    11941 gcgctggcta tccctcgacg tgttgcacga gccggcgcac aggatgctga tagctctcta
    12001 cgccttctcc gaccagcgtg ccgccgccct gcgccagtac agggagtgcg tgaggatcct
    12061 ggaccgggag ctcggcgtgg ccccgctaga ggagacgacc ctgctgtacc gggccatcca
    12121 ggagaacgac ctcccgccaa gaccgaccct ctccgaaccc atgccggcga tgggacggga
    12181 agcgcctccc gcgaagacga cggtcaccgt gccctcttcg cccgaaaacc ccctggtggg
    12241 gagggacccc gaatgggttg ccctgctcgg agcttacgcc tccatcggag aggggggacg
    12301 catcgtcgtc atagagggcg aggccggcat tggaaagacc aggctggccg aagagttcgt
    12361 ggcgcacgcg ggtgagggtg gcgccacaac cgttgtcgcg cgctgctacg cgggcgagaa
    12421 gaacctggcg tacgggccct ttatcgaggg gctctccgcc gccctcggac gggagagcgg
    12481 ggatcggctc gcggacctac cggcctcctc cctgcaggag gcggcccgtc tcctgcccgg
    12541 gctagcgccc gactcgtcgc ccgccccgcc gctcgacaca ccgggtgcca ggacccgttt
    12601 cttcgcggag gtgatccggg tcttgcaggc catcctggac ggcccgcccc ctggtgtgct
    12661 cttcctcgac gatctgcact gggccgacga cgcctcgctg ggcttgctca actacctcat
    12721 ccggcgcctc gacgacaaac cgttctgtgt gctcctcacc tggcggacgg aggacgtccc
    12781 cgaaaagcac cgcctgcggg agctacttgc tgaggccagg agatccggca cggtcacggt
    12841 gctctcgctg gagaggttga gcccccgctt cgtggaggag ctggtcggta acaccgtgcc
    12901 cgacgcggcg gggctcggcc cacggctcag tgaagagacg gaggggctac cgctcttcct
    12961 cacggagtac ctggcggccg tcgagaaggg agagttggat ccgggcgacg aggcctggac
    13021 gctaccggga ggggtacagg acctgctcag ggcccgcctc gggaccgtcg acgaggcgtc
    13081 cgggcagatc ctcgccaccg ccgccgtcgt cggaaggtcg ttcgactttg acaccgtaag
    13141 ggcagcgagc ggtcggggcg aggaggagac acttgatgcc ctggaggagt tgatctcgcg
    13201 tggcctcatc agggaggttg ggggggcggg ggcaacccct tcatacgact tcgaccacga
    13261 caagctaagg accctggtct acgaggagac cagcctggcg cgcaagcgcc tcctgcaccg
    13321 ccgcaccgca gcggcgctcg ccggcggagc ccgcgggcgc gaggcgagct ccctcgcggg
    13381 ccagatagcc cgccatcaca ggctcgccgg ccaggatctc gaagcagcac gctactacct
    13441 gctggccggc gactacgcgc gcagcctgca cgccaacagc gaggccctca gccactacga
    13501 agaggcgctc gcgctcgggt atcccgaggc cgccgcgctg cacgagtcca tcggggacct
    13561 caggacccgc acaggcgagt atggtgcggc gctcgccagc tacgaagccg ccgcctcatc
    13621 cgcagggccg ggtgatctgg cggagctcga gcacaaaata ggcaacgtcc acgcccgcct
    13681 gggggagcgg gatctcgccc gcctccatta cgaatccgcc gtgaacgagt tctctcagac
    13741 ggacagcgag gccgagctcg cgcggctcta cgccgactgg agcctgctcg cccacggaca
    13801 ggaccaacca gaagaggcag ccgccctcgc ccggcaagcc ctggccctgg ccgagatcac
    13861 gggcgacacg cgggccctcg cccaggcgca caacatgctc ggcatactgg ccggcaaagc
    13921 gggagaccag gaggcggcgc tcagccacct cgccaagagc ctcgatctgg cagaggccct
    13981 tggcgactcg gacgcgcggg tcgccgccct gaacaacctg gccctcgccc gggagggcgg
    14041 cggagaacca gaagaggccc tgaaactagc cgaggcagca ctcaagctgt gcgagacctc
    14101 cggcgacagg cacagggagg ccgcgctgca caacaacctc gccgacctgt ttcacgccac
    14161 gggccgcgaa gtggaatcca tggagcacct caaacgcgcc gtagagatct tcgccgagat
    14221 cggcgagcga ggcgagctgc agccggaggt ctggaagctc gtcgaatggt aagaatggta
    14281 aaggagcttt cagccgtttg cctctggccc cttaccggct gaagacgcga aagcttctct
    14341 cttggcggca ttgatctcac cccaggggat gctgggtcgc gcgagcccgc gcttctctag
    14401 atagcttctg gcgttgcgtc gcgatctgac aaacagacgt atcatgcccg gtagtcctcc
    14461 catgccgata cctgggacga accgcacggg ctcgaagctc cattgcccga aatcaaggaa
    14521 cagtacgccg cgaacgagga agcccctcag attccagagg ttctcgtcgt gactgtggca
    14581 gagcaggctt gcggggccgg gccgaaaggg agtaccaggg ggaagccata ccctgagcac
    14641 ctcggcgccc gatgcatccg gatagggacg gcagcgcacg ctgaacgggt agccgtcctc
    14701 gtcgacgccc gataagacgg cgctctcgaa ctcgtacagg tgcgtggtga tctcaggcca
    14761 catgatccat cctcaactcg aaaggctccc gccagaaatc tgcccccggc caccacatga
    14821 tgcggcgggg cgtcacgtgg atcatgagcc gcataaagta ccagtccatg aggcgacgtg
    14881 taagcggatc gttgccgtaa aagcgggagg ccggctggcg ggggtagacc ttacgcagct
    14941 cctcctcgaa gcccacggcg gaggtctcta tctcgtccgg ggccacggcg tcgccctgca
    15001 ccaataccgc cggcgggtcc tcgaggccgc tagccgtcgg gttggagaac agcagcgaga
    15061 cccgcgggtt gcgccggacg ttgagcgctt tctgggcgaa gccgatggat gcagttatcg
    15121 tgaagtgctt tttatcggag taccagaagg gcaacgtggg ccacgtaatc ggggtaccgt
    15181 ccctggccag cgtggacatc tcgcacgtcc tgaactcctc gaaaacatct tctacctcgg
    15241 gcggaagccc tgcgacgatg cgcgatgacc ggggcacgat ccactccttt tctcgtcctc
    15301 gacatatcct gctacctcat ccaccatcca agaacggaaa ccttcatccg catccccttt
    15361 ccggcgtgaa ctttcatgcc tccaatctag cccacccgcg ttacgccccc gttacgaaaa
    15421 ccgttacgaa gagacaaaat cgaagatttt gtcagcacgc tcgggccttc ggcctacgct
    15481 tgtcagcacg ccgcttcgcg gcttgtcagc acgcttcgac cctgcgggtc ttgcttgtca
    15541 gcaacgcttc ggcttcgcct cgctttcggc cggtcgtttc ggttcgcatc ggtggcttcg
    15601 gcaacccatt cttgtcgagc atcgaatagt tagagcagaa gggggcaggc cgcagcggga
    15661 tcggctgaaa gcgaccgaag ggagcgtgct gaaagtggag gcccgaaggg ccgtaacgtg
    15721 ctgacaagcg agggcaccag cccgagcatg ctgacaagcg gaggcgttag ccggagcgtg
    15781 ctgacatggc cgttcaggcc atgtcgttgt gttcccattc tgtggctacg ggacgttcgg
    15841 gggcacgcca ggtcggtgct gttgggatca gatcgagttg gcgggtgagt tgcctggcgg
    15901 cgaggccgat tatggcggtg aaggtggggt aggcaagctc caggttggcg agctgttcta
    15961 cgcgcatgcc ggcgcccatg gcggtggccg ctagctggac gacctcgacg gcttgctctc
    16021 ccacgatatg ggccccgagg acgcgccgcg aggagcggga gacgatgagc ttgcaggagc
    16081 cgtcgggtcg tccgtctatg accccacggt cgaggtcggt gtagggcacc acagcgacgg
    16141 cgcagtcccc gtcgccgtac tcgtcgcggg cttggcgctc ggtgaggccg acgctcccgt
    16201 actcagggtc ggtgaaaccg ccgtgcggaa cgatggcgtg cctgtagcca cggtcctctc
    16261 ctagcacggc gttctcggcg gcgaggcttc cctcgtagtt ggcgctctgg accagcatca
    16321 tccgccccgt caggtctccc gcggcgaaga cgtgcggcgc gcttgtcctg agacagtcgt
    16381 ccaccgtcac gtaacccctt cttgtctcta tccctgccgc ggcgaggttc agcctatcga
    16441 cgttgcccgg ccagccaacg gcaagcacaa ccgcttccgt ctcgagcacg cgtgtctcgc
    16501 cgtcctgcgt gtaggagagg ttgagaagct tgccttcccg gtctatgcgg tccacgcccc
    16561 cgatgcccgt gatgatctcg atgtccctgc gtgagaacac ctcggctacg ccctccgaca
    16621 ccaccccgtc ctcgccgccc aggatgtgcg ggccaacctc gagcagtcgg accctggagc
    16681 cgaacgccgc gaagacggag gcgagctggc agccggtggc ggcggcgccg acgaccacga
    16741 ccgaggaggg caggctctgc atcgcccaca cgtcgctgtg ggtcagggca tgttcggaac
    16801 ctgggaagtc catccgccgg gcgtggccgc cggcgcacac cacgaacttc tcacccgaga
    16861 cgcgggagcc
//
LOCUS       DBNGVD010000568         5415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1244136, whole genome shotgun sequence.
ACCESSION   DBNGVD010000568 DBNGVD010000000
VERSION     DBNGVD010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1244136"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="AC16CM_15280"
     CDS             <1..427
                     /locus_tag="AC16CM_15280"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3141242.1"
                     /translation="WEDLMPEINRATDPTNMIWKLVDRETGAENHQISWKFTVGDRVK
                     IRLVNEMESDHPMHHPFHIHGAGRFLILSRDGRPEPNLVWKDTLLLRAGETVDVLLYV
                     TNPGLWMAHCHIAEHIEGGMMFSFEVAPPSEEARGGEPR"
     gene            605..1753
                     /locus_tag="AC16CM_15285"
     CDS             605..1753
                     /locus_tag="AC16CM_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141243.1"
                     /translation="MTEGARERGRGAGGMGARAAAWLAWSVCAFSLVLTALGLAFLGL
                     NRSHPGVPVFEFAFRATVITASCSSVGVIIASRRPAHPIGWLFGAVGLLSGVHLFSGE
                     YAIYALAVGRGELWGGGVAAWITCWLWVPINALLAFVALLFPDGRPPSPRWRALGWIN
                     GIVAVAGSFTAAFLPGPSPWLPAMDNPLGVEGLRDARNLLDASLEALSYGVLVAGVVS
                     LYFRYRRAGRVEHQQIKWLAYVGAVLLSGTILLNAGPDSLEGTWIKQLGFAMWVVGFA
                     GVPIAIGIAIFRYRLYEIDVIINRTLVYGALTVVMATVFESIDATLHYLLVTLAHVHT
                     LFGSIIAALVVGALFDPVRHRIQRFVDGRLRTGEEDGGPAQPEGEVPS"
     gene            complement(1824..2198)
                     /locus_tag="AC16CM_15290"
     CDS             complement(1824..2198)
                     /locus_tag="AC16CM_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3141244.1"
                     /translation="MDEFVNGLVWVGTRTQHYEEMVAFYRDTLRLPLDHEEGEFAIFR
                     LPDGSKAEVFGPSDTEHAHFSTGPVAGFLVDDVESARRRLEAGGIEFVGPVHEWEPTG
                     EAWSHFRAPDGNVYEIVQGPKR"
     gene            complement(2474..3208)
                     /locus_tag="AC16CM_15295"
     CDS             complement(2474..3208)
                     /locus_tag="AC16CM_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3141245.1"
                     /translation="MRTIKTMAAAFVLMLAFYVSLTAGTASAKTLDGTSYGDALYGTA
                     YGDTIYGYGGSDLAYGYAGTDLLYGGNEIGSGDKLLGGAASDQVLGQKGDDALYGQNG
                     GDEVTGGYGHDLVVGGDSEDILDGGPGSDEINARDGQKDTVIIRPGEYDTVYYDQGLD
                     VLQEQTVSQEGTDRSSELIASGTGKTELVAKRPPEGLFGHTGEVLVEHGGEGLLVAEG
                     ELEDHLGHGDGIIDPTGRAAAGQGRS"
     gene            complement(3554..4414)
                     /locus_tag="AC16CM_15300"
     CDS             complement(3554..4414)
                     /locus_tag="AC16CM_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3141246.1"
                     /translation="MFALEPASSDAPRIGVEWLDRGHEMALSGPHGHRFLALTYFERG
                     GGRQRIGSQLWDVSAGDFFVIAPGETHEAGELGEAQGWMVQFLADAIEPGGDPGALLS
                     WQSNPLLYPFVRPSGGEVARFKVPAERRSEWSRRLRSMRAELRDRSPGFREALRAHLA
                     LILVDVGRLAQDVVGQMRLGDQPVLAEIFRFIEDRYAEPISTKDVARAVNLSSGYLTT
                     LVRRRTGRTVLEWIVERRMAEARRLILETDESIERIGARVGYEDPTYFARRFRSAHKA
                     TPAAWRNANR"
BASE COUNT          839 a         1818 c         1761 g          997 t
ORIGIN      
        1 gtgggaagat ttgatgcccg agatcaaccg tgcgaccgat cccaccaaca tgatatggaa
       61 gctcgtggac cgggagaccg gggcggagaa ccatcagatc tcctggaaat tcacggtggg
      121 cgacagggtg aagataaggc tggtcaatga gatggaatcg gaccatccca tgcaccaccc
      181 cttccacatc cacggggccg gacgattcct gatcctctca agggacggcc ggcccgagcc
      241 caacctggtc tggaaggaca ccctcttgct gcgagcaggg gagaccgtag acgtattgct
      301 ctacgtcacg aacccggggc tgtggatggc ccactgccac atagccgagc acatcgaggg
      361 cgggatgatg ttcagcttcg aggtcgcgcc cccttccgag gaggcacggg gcggcgagcc
      421 ccgctaaggc gccgacctcg gtgcgcggtc gcgatcttca ctcaggggtg ctccggaaag
      481 cggctcgggg caaggcgcgt gcggcgcacg gagcgctaag ggtgaccgac ggcgttcgcc
      541 aaccgctccg ccatgcggct accgccgtga gatctggaag gcgatcgtct tgtagggtgg
      601 cgccgtgacc gaaggggcaa gggaacgggg ccgaggagcc ggcgggatgg gcgcccgcgc
      661 cgcggcctgg cttgcctggt ccgtgtgcgc cttctcgttg gtccttaccg cgcttggcct
      721 cgcgttcctg gggctgaacc gctcccaccc gggcgtgccc gtgttcgagt tcgcgttccg
      781 ggccacggtg ataacggcga gctgctcgtc ggttggcgtg atcatcgcct cccggcgccc
      841 cgcccacccc atagggtggc tcttcggcgc ggtgggcctc ctcagcgggg tgcacctgtt
      901 ctcgggcgag tacgccatct acgcgctggc ggtggggcgc ggggagcttt ggggcggcgg
      961 ggttgcggcc tggatcacct gctggctgtg ggtgcccata aacgcgttgt tggcgttcgt
     1021 ggcgttgctg ttccccgacg gcagaccgcc gtcgccccgc tggcgagccc tcgggtggat
     1081 aaacgggatc gtggccgtgg ctgggagctt cacggccgcg ttcttgccag ggccgagccc
     1141 ctggcttccg gctatggaca acccgttggg cgtcgagggc ttgcgggatg ccaggaatct
     1201 tctagacgcc tcgctcgagg cgctttctta cggcgtcttg gtagcggggg tggtctcact
     1261 gtacttcagg taccgacgcg cggggcgggt ggagcaccag cagatcaagt ggcttgccta
     1321 cgtcggggcg gtgcttctta gcggcacgat attgttgaac gccggacccg actccctgga
     1381 ggggacctgg atcaagcaac tcgggttcgc gatgtgggtg gtaggcttcg cgggcgtccc
     1441 gatcgccata ggcatagcga tcttccgcta ccgcctctac gagatagacg taatcataaa
     1501 ccgtaccctc gtctacggcg ccctcacggt agttatggcg accgtcttcg agtccatcga
     1561 cgcgacactc cattacctgc tggtcaccct ggcgcacgtc cacacccttt tcggctcgat
     1621 aatcgccgcc ctggtcgtcg gcgccctctt cgatcccgtg agacaccgca tccagcgctt
     1681 cgtggacggt cgcctacgca caggagaaga ggatggcggt ccggcacaac ccgaagggga
     1741 agtgccttcc tagggcttct tgggcaccgg caacattccc gcgcttgcgc cggtcgccga
     1801 actcgtaccg gcttcccgta tgatcaacgt ttcgggccct gcactatctc gtagacgttc
     1861 ccgtcggggg cccggaagtg cgaccaggcc tcccccgtgg gttcccactc gtggacgggt
     1921 ccgacgaact cgatacctcc cgcctccagg cgcctgcggg cgctttcgac gtcgtctacg
     1981 aggaacccgg ctacggggcc ggtggagaag tgggcgtgct cggtgtcgga tggtccgaaa
     2041 acctcggcct tgctgccgtc gggaagcctg aagatcgcga actcgccctc ttcgtgatcg
     2101 agcgggagcc gcagggtgtc ccggtaaaag gccaccatct cctcgtagtg ttgcgtccgt
     2161 gttccaaccc acaccaaccc attgacgaac tcgtccatgg cttcctcccg tcgaccgttt
     2221 ctcgttcgct gtctgaatcc ccgcccggct tccacggcgg cggggtactc ctcacctgtc
     2281 ggcatgatgc catgctcgga cgccacgcgc cggtgcggaa taccgatacg ccccgattcg
     2341 cagaagcggc gagtgaggcc ggacagagcc gaggttccca aggggcacga ggagtcgaag
     2401 gcgagagccg gggtcctaaa ggaccccggc cccgacccaa tagggccgac tcgcgccttg
     2461 ccagaagctt gacctaactg cggccttgcc ccgccgcggc gcgccccgtc gggtcgatta
     2521 tcccgtcgcc atgcccgaga tgatcctcaa gctccccctc ggccacgagg agcccctcgc
     2581 ccccgtgctc caccaagacc tcgccggtgt gcccgaagag cccttccggc ggccgcttgg
     2641 cgaccagttc ggtcttgcct gttcccgagg cgataagttc ggagctcctg tcggtaccct
     2701 cctgggagac cgtctgctct tgcagcacgt cgagtccctg gtcgtagtag acggtgtcat
     2761 actcaccggg gcggatgatc acggtgtcct tctggccgtc ccgagcgttt atctcgtcgg
     2821 agccggggcc gccatccagg atatcttcgc tgtcgccgcc gaccacgagg tcatggccgt
     2881 agccgcccgt gacctcgtcg cccccgttct ggccgtagag cgcgtcgtcg cccttctgcc
     2941 cgagcacctg atccgacgcg gcgcctccca gcaacttgtc gccgctgccg atctcgttgc
     3001 ccccgtagag gaggtctgtg cccgcgtagc cgtacgcaag gtcgctgccc ccgtagccgt
     3061 agatggtgtc tccgtaggcg gtgccgtaca gggcgtcccc gtagctcgtg ccgtcaagcg
     3121 tcttcgccga ggccgtgccg gcggtgaggc tgacgtagaa ggctagcatc agtacaaaag
     3181 ccgcggccat cgtctttatg gttctcatcg ttacccgctc ccttccttgg tgtatgaacg
     3241 tcttcgtttc ggttgttctc atcatcttcc tcccgtttcg tcttttgagc tcgtcggtcg
     3301 tctgccgcat ctgccgtcct ccttcgactc gatccgttcg cgcctccttc ccgatccttg
     3361 cgcttgcctg tcatcgcctt cgatcgtgat ggtgccacct tcacagcacc ccgctaaggg
     3421 acgatcgtcg tgggccatcg gacgattctt ggtttctact tccggggagt cgaagaggcc
     3481 cacgaaacgg cgcgcggtgg acgacgcgag gagaaggaca ccctcgcgcc ggtgggcgag
     3541 accagggaac gcgtcagcgg ttggcgttgc gccaggcggc gggcgtcgcc ttatgggcgc
     3601 tccggaagcg gcgggcgaag taggtgggat cctcgtagcc gacgcgggcc ccgatccgct
     3661 cgatgctctc gtccgtctct aggatgaggc gcctcgcctc ggccatgcgg cgctcgacga
     3721 tccactccag cacggtgcgc cctgtccggc gcctgacgag cgtggtcagg tagccggagg
     3781 acaggttcac ggcgcgtgcc acgtccttgg tcgagatggg ctcggcgtag cggtcttcga
     3841 tgaaccggaa tatttcggcg agcacgggct ggtccccgag gcgcatctgg ccaaccacgt
     3901 cttgggcgag gcgcccgacg tcgaccagga tcagcgcgag gtgcgcccgg agcgcttcac
     3961 ggaagccggg acttctgtcg cgcagctccg cgcgcatgga gcggagccgc cgcgaccact
     4021 cggagcgccg ctccgcaggg accttgaaac gggcgacctc ccctcccgag gggcgcacga
     4081 aggggtaaag cagcgggttg gattgccacg agagcaaggc cccggggtcg ccgccgggct
     4141 cgatggcgtc ggccaagaac tggaccatcc atccttgggc ctcgcccagc tcgcccgcct
     4201 cgtgggtctc gccgggcgcg atcacgaaga agtcgcccgc agagacgtcc cacaactgag
     4261 acccgatccg ctgccgtccg ccgccccgct cgaagtaggt aagggcaagg aagcgatggc
     4321 cgtgcggccc cgagagggcc atctcgtgcc cccgatcgag ccactcgacc cctatgcgcg
     4381 gagcgtcgga ggaggcgggc tcgagcgcga acatgggatg tttgcgcctc gttcctccgc
     4441 tcgccaaagt ggtccctccg tgcccgaaac gggtttggtg ttcggccgta gatgcagtca
     4501 gggagtatcg catcgtcttc gcgcgagcgc gcggcggtcc ccccggtcct cgcgatgccc
     4561 agcgagccgc tggtagatga ccgtctgctc ctgtctttac gcgtctccgc gacacatcct
     4621 cggttccgag gggcgccggt cccgccaggc ggctgacccg ccgcgcaggc cgtcggaccc
     4681 tgagggatca tcgatgctcg cgccgggaca cgggtcgtgt tctctcgcgg cgagcggcgg
     4741 gaccccggcg tcagcgaggg aggtactcgt cgaccgcccc gcaacaccgc acgatggccg
     4801 acgcgaccag cacgagcatc gcgccccgta gcttctccat cttgtcgtct acacctcccc
     4861 tccgcgactc gtcttcggcc aacgacgaga ccggttttct gcacgctctg ctctcgtccc
     4921 gcgtcggtac tcgctcggat tgctgggcgt ccctctccac gcaccaggcg tcaggtttcg
     4981 gggcgtcgca gaagggcgcc tccggtttcg agttcagtgt cgcgcctccg ggcgggtcgc
     5041 cgtcctcggt gaggtcgctt gcgtgccgcg ctccaggacc tgcctgggct gacgcgagag
     5101 gtcacgcggc ccgtagacga gaaccacgac gacggctcgc aaccccgata gcatgatctc
     5161 tcaccggggt gcatattcgg ccagcatgct gacctggggg atttctccga acttcgccca
     5221 gagggcgtac tcggacgttc gcaacacctg acgaccataa gcaacgacaa atatatgcca
     5281 cccggcgcat atacattccg tgagtcggtc tagatacttt tgttgctata gcaaacgaag
     5341 gcggggaagc tcttcctccg ccccacataa gacaaggagc acaaagatca tgatcaggag
     5401 cgctgcgaga aaggt
//
LOCUS       DBNGVD010000569         3969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2155834, whole genome shotgun sequence.
ACCESSION   DBNGVD010000569 DBNGVD010000000
VERSION     DBNGVD010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2155834"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC16CM_15305"
     CDS             complement(<1..375)
                     /locus_tag="AC16CM_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141247.1"
                     /translation="MGNIGEHPHEVHHHHIPFARHDDPDAEVAERWIIAAAIIGSSMA
                     FIDGTVANVALPSIQNDLGTTLATAQWVVQAYALLLATLILVGGSLGDRFGHRLVFVL
                     GILIFTLASVGCAFAASMNQLIA"
     gene            complement(388..630)
                     /locus_tag="AC16CM_15310"
     CDS             complement(388..630)
                     /locus_tag="AC16CM_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141248.1"
                     /translation="MEREETGNVDGMRDDAPQMSHFFGRGSLVTVSARTGNDLKGYVC
                     DHSENGLLLDSRDPSGDPTAYEFLPWSSIERVSAAE"
     gene            complement(783..1370)
                     /locus_tag="AC16CM_15315"
     CDS             complement(783..1370)
                     /locus_tag="AC16CM_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3141249.1"
                     /translation="MPRMPTLCVDVDSTIWDTGAWICAAVLEHTGEELDVEEVTTWTH
                     LLDAYGEETTTRVFDRVFDPARIREREPYPGAPEALRALREEFGIGIHFVTHNDPETM
                     TSRLHPWLADLFGPDVGLTVTTGDKLEIIRDLKAFGLIDDRPDTIERVADADLWAATR
                     IQPWNRDLVARRTDVHGFHHWQEVRDLLLQRLSSR"
     gene            complement(1453..2106)
                     /locus_tag="AC16CM_15320"
     CDS             complement(1453..2106)
                     /locus_tag="AC16CM_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141250.1"
                     /translation="MDEMTPELALEDLPRPPLFKLVEPGGEDVFQETEVGGEGALVGV
                     LFSDRELAGEFSTGAAAHGMESLSNLDPRQLPDWGSVEDFALSGADFLLVVSESGAGL
                     FHSADVAQKARESAGEIPYPLYMISDERGEAPLISVEVDSGEVLVAALFSSREKAREF
                     RAMAAHLDLPDAHGTIEDRDGLRRHALVAREAGADYAVIDPASGLTEAIPIEELIAP"
     gene            complement(2099..3604)
                     /locus_tag="AC16CM_15325"
     CDS             complement(2099..3604)
                     /locus_tag="AC16CM_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3141251.1"
                     /translation="MSRRRKILITLATLVGTFLAALDSTVVGTAMPTVIGELGGLELY
                     PWAFASYLLAATVTGPIFGKLSDTHGRKPVYLAGISLFLLGSVLAGTSQSMGAFILFR
                     TLQGLGAGAVQPVAVTIMGDIFELETRARVQGLFGAVWGISAVVGPAVGGFITDLVSW
                     RWVFYVNVPFGIVAAVLLAATLTESFDRRERSQDYLGTVLLAGGLVAVLLALLGGGLS
                     VATLALFLGGLIVLSLFVAVERRAADPVVPLDLFSERIIAVSSLGNVAMGGVLLGVSV
                     YVPLFVQGALGGTALTAGAAVASLSIGWPVGSFVGGRLLLMTGYRAMLLLGSALLALG
                     SALCVPMDGGTSLTYVVVAVVVIGLGLGFSSTSYLVSVQNAVPWHRRGVATSSVVFFR
                     TVGGSLGVAVMGALLNASLGERYRAAVDRAAGGDEGLGRLLSDPNALLQPAVRSRIPD
                     AAYSELSGALAASLSPAFWVLLFFGLLALVVATFFPRGSAKDLANRAEDYG"
BASE COUNT          734 a         1332 c         1295 g          608 t
ORIGIN      
        1 ggcgatcagc tggttcatgg aggccgcaaa cgcgcacccc acgcttgcca gcgtgaagat
       61 caggatgccg aggacgaaga ccagccggtg gccgaaccgg tcgccgagcg agccgccgac
      121 caggatcagg gtggcaagca acagcgcgta ggcctggacc acccactggg ccgtcgcgag
      181 ggtggtcccg agatcgttct ggatggaggg caaagccacg ttggcgacgg ttccgtcgat
      241 aaaggccatg ctggagccta tgatggccgc ggcgatgatc caacgctccg cgacctcggc
      301 gtcggggtcg tcatgccggg cgaacggtat gtggtggtgg tgaacttcgt gtggatgctc
      361 gcctatgtta cccacgtctg accccgttta ttctgcggcg ctcacccgtt ctatgctcga
      421 ccagggcagg aactcgtagg cggtcgggtc accgcttggg tccctggaat cgaggagcag
      481 gccgttctca ctgtggtcac agacgtagcc cttcaggtcg ttgccggtcc tggccgagac
      541 cgtgaccagg ctaccgcggc caaaaaagtg cgacatctgc ggcgcatcat cccgcatacc
      601 atctacgttt ccggtctcct cacgttccac ggcgtctcct taacgtgtcg cttgggcaca
      661 caacagggta cagcacgctc cggcctgcgg cccgcgcggc gcgctcgaag cggtcagtag
      721 cctcgctttc ggagctgcgc tcgtgtccct cgggggtggt cgagacagga ttgtcccggg
      781 ggtcaacgtg aggacagccg ttgcagcagg aggtctcgga cctcctgcca gtggtggaaa
      841 ccgtgaacgt cggttctgcg ggccacgagg tcacgattcc acggctgtat ccgtgtcgcc
      901 gcccaaaggt cggcgtcggc gacccgctct atggtgtctg gcctgtcgtc tatgaggccg
      961 aaagccttca ggtcgcggat gatctccagc ttgtcacctg tggtgacggt cagcccaaca
     1021 tctggtccga agaggtcagc cagccaggga tgcaggcggg aggtcatggt ctccgggtcg
     1081 ttgtgggtta cgaaatgtat cccgatgccg aactcttccc gcagggcacg cagagcctcg
     1141 ggggcgccgg ggtacggttc cctctcgcgg atgcgggcgg ggtcgaagac gcggtcgaag
     1201 acgcgggtgg tggtctcttc gccgtaggcg tcgaggaggt gggtccaggt agtaacctcc
     1261 tccacatcca gctcctcgcc cgtgtgctcc agcaccgccg cgcagatcca cgcgcccgtg
     1321 tcccagatgg tcgaatcgac gtccacgcag agggtcggca ttctcggcat cacgaacctt
     1381 tgacgctact cgacgggccg gggacagaac tgctgtctgg cctggcccag ggtattgcgt
     1441 gtcgtggtgg gctcacggcg ctatcaactc ctctatcggg atggcttcgg tcagccccga
     1501 ggcagggtct attacggcgt agtcggcccc ggcttcgcgg gcaacgaggg cgtggcgcct
     1561 gaggccgtct ctgtcttcga tggtcccgtg ggcgtcgggc aggtcgaggt gggcggccat
     1621 cgcgcggaac tcgcgggcct tctctcgtga gctgaacagg gcagccacca gcacttcccc
     1681 gctgtcgacc tcaacggaga tcaggggcgc ctctccccgc tcgtcggaga tcatatacag
     1741 cgggtagggg atctccccag ccgactcccg cgccttctgc gcaacatctg cggagtggaa
     1801 caggccggcc ccactctcgg agacgacgag caggaagtct gctccggaga gcgcaaaatc
     1861 ctccaccgat ccccagtccg gaagctgcct cgggtccagg ttggagaggc tctccatccc
     1921 gtgcgcggct gcgcccgtgg agaactcccc ggccagctcc ctgtcggaga acaggacgcc
     1981 gacaagggcg ccttcgcctc cgacctcggt ctcctggaac acgtcttctc cccccggctc
     2041 aacgagcttg aacagtggcg ggcgggggag gtcttcaagg gccagctcgg gcgtcatctc
     2101 atccataatc ctccgcccgg ttggccaggt ctttggccga gcctcgcggg aagaacgtgg
     2161 ccactacgag ggcaaggagg ccgaagaaca gaagcaccca gaacgccggg ctgagcgagg
     2221 ctgcgagggc gcctgacaac tccgagtagg cggcatcggg gatgcggctg cgcacggcgg
     2281 gttgcagcag ggcgttcggg tcggagagca gcctcccgag gccctcgtcc cccccggcgg
     2341 ccctgtccac ggcggccctg tagcgctccc cgagcgaggc gttcagcaag gcgcccatca
     2401 cggcgacccc gagcgagcct cctacggtgc ggaagaacac caccgaggag gtggccacgc
     2461 cacggcggtg ccaggggacc gcattctgca cgctgaccag gtagctcgtg ctggagaagc
     2521 cgaggccgag ccctatcacc acaacggcaa ccaccacgta ggtgagggag gttccaccat
     2581 ccatcggcac gcagagcgcg gacccgagcg cgagcagggc cgatccgagc aacagcatcg
     2641 cccggtaccc ggtcatcaac agcaacctcc ccccgacgaa agaccccacg ggccacccga
     2701 tagagagcga ggcaaccgcc gcgccggcgg tcagcgccgt gccgcccagg gccccctgca
     2761 cgaacaacgg cacgtacacc gagacgccaa gcagcacgcc gcccatggca acgttcccga
     2821 gggaggacac agcgatgatc ctctccgaga agaggtcgag cggaaccaca gggtcggccg
     2881 cccgacgctc gaccgccacg aagagggaga gcacgatcag cccccccagg aagagggcca
     2941 gggtcgcaac agacaggcct cccccaagca gcgcgagcag gaccgcgacg agcccgccag
     3001 ccaggaggac ggtgcccagg tagtcctggc tgcgctcacg acggtcgaag gactcggtca
     3061 gggtcgcggc gaggaggacg gcggcgacta tgccgaaggg cacgttcacg tagaacaccc
     3121 agcgccatga gacgaggtcg gttataaagc cgccgaccgc cggcccgacc acagccgaga
     3181 tcccccagac cgccccgaag agaccctgaa cccgggccct cgtctccagc tcgaagatgt
     3241 cccccatgat ggttacggcc acgggctgca cggcccctgc cccgaggccc tgaagggtgc
     3301 ggaagaggat aaacgccccc atgctctgag aggtgccggc gagcacgctg ccgagcaaga
     3361 acagggagat gccagccagg tacaccggct tcctgccgtg cgtgtccgag agcttgccga
     3421 agatgggccc cgtcaccgtc gccgcgagca ggtaggaggc aaaagcccac gggtagagct
     3481 ccagtccgcc aagctccccg ataaccgtcg gcatcgccgt cccaaccacg gtcgagtcga
     3541 gagcggccag aaacgtcccg acgagcgtgg caagcgttat gagaatcttg cgtctcctgc
     3601 tcaaacccaa agaaacagcc ctcttccgct accatgccct gttcgaggcg cgatcttacc
     3661 atcgccgcct cggccagaag gttagcccgc ccagaagcat cgcgcagtaa agcacgctac
     3721 ggccctgcgg gcctccgctt gtcagcacac tgcggcctgt cggcctccgt ttgtcagcac
     3781 gctcgggctg acgccttcgc ttgtcagctc gcttcgcctg gctcgctttc agccagatcc
     3841 cgctgcggcc ttcacccttc cgcttcaacg attcgatgct cgagaaaggt gggtagtcga
     3901 agccactgtt gcgaaccgag acgaccagcc gaaagcgagg cgaagccgaa gcgttgccga
     3961 caagcgaga
//
LOCUS       DBNGVD010000570         2574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1609106, whole genome shotgun sequence.
ACCESSION   DBNGVD010000570 DBNGVD010000000
VERSION     DBNGVD010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1609106"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="AC16CM_15330"
     CDS             complement(<1..847)
                     /locus_tag="AC16CM_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141252.1"
                     /translation="MWVARVAWVLTAITVLGLDAAGLPHAYDRLSTVCHSSGCSGSEE
                     ILSRADVGDLRDVGISPDLYAGLTVGLSTLGTVVFAATAAAIFFRRSDDKMALLTSFT
                     LLIFGGAAFTGTMQYLVGDYPSLWWPINLLEYVGQMLFNVFFLTFPDGRFVPRWTRWI
                     AVMWAVLFVPYVFLGGTSLDILSSPLFIVYVASLVFAQVYRYAKVSNLVERQQTKWVV
                     FGFATAFGGFAATVVIGDVVPGVRQYEAIAQIISGVLIYAFILLIPISISVAVLRSRL
                     YDIDVV"
     gene            complement(895..2175)
                     /locus_tag="AC16CM_15335"
     CDS             complement(895..2175)
                     /locus_tag="AC16CM_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141253.1"
                     /translation="MSRRGALWFAWVLAGISFALYASAFVFAFLTLGVDEPVKQVSTG
                     GFGELLVYLPLLAFPIVGALIASKRPGNPIGWICLVSGLFWMSFAQGDASKAYELART
                     GTVTSSVTLDALLQGSWTLPVGLLGVYMVLLFPDGRLPSRRWRPFALFAGAVIVLIPI
                     LFVFVPGQLEDHPGVRNPFGLEQYPWLESVAVFGVLLLPTCIVVSAVSLVLRYRRSGA
                     DVREQIKWLAFAASVVGISYLSAVITGIFFLPELFSPQVPRNLLYALIPNLVLMSYAG
                     IPIAIGFAVLKYRLYDIDVIINRALVYGSLTVMLAAVYFGGVVGTQAVLGTLTGREQQ
                     PQLAVVVSTLAIAALFTPLRRRIQAFIDRRFYRRKYDAARTLDRLSLRLREEVDLENL
                     VGELVAVVRRTLEPTHVSLWLSDGEERSRMRKYR"
     gene            complement(2259..>2574)
                     /locus_tag="AC16CM_15340"
     CDS             complement(2259..>2574)
                     /locus_tag="AC16CM_15340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3141254.1"
                     /translation="TFDMTGRIEAQIERFAPGFGERVLAKRAAGSTELERWNPNLVGG
                     DINGGYMDLRQLFTRPMPRPNPYSTPARGLYICSSSTPPGGGVHGMSGYLAARVVSRY
                     LA"
BASE COUNT          558 a          808 c          828 g          380 t
ORIGIN      
        1 tcaccacgtc gatgtcgtag agccgcgagc gaagcaccgc gacgctgatg gagatcggga
       61 tcagcagtat gaaagcgtag atcaagacgc cggagatgat ctgcgcgatc gcctcgtact
      121 ggcggacacc gggaacgacg tccccgatga cgaccgttgc ggcgaaccca ccgaaggccg
      181 tggcgaaacc gaataccacc cacttggtct gttggcgctc taccaggttc gacactttcg
      241 cataccggta gacctgggcg aaaaccagcg aggccacgta cacgatgaac agggggctgc
      301 tcaagatgtc gagggaggtg ccacccagga acacgtaagg cacgaacaac acggcccaca
      361 tgaccgcgat ccagcgggtc caccgcggca cgaaccgccc gtcggggaag gtgaggaaga
      421 agacgttgaa caacatctgg cccacgtact caagaaggtt tatgggccac cacagcgagg
      481 ggtaatcgcc aaccaggtac tgcatcgtcc cggtaaacgc cgccccaccg aagatcagca
      541 aggtgaacga cgtcagcagg gccatcttgt cgtccgagcg gcggaagaag atcgcggccg
      601 ccgtcgcggc gaagaccacc gtgccaaggg tcgagagacc gaccgtaagg ccggcgtaga
      661 ggtcggggga gatcccgaca tcccgcaggt cccccacgtc ggcccgggag agtatctcct
      721 cggagccaga gcacccgcta ctgtggcaca ccgtgcttag cctgtcgtag gcgtgcggca
      781 ggcccgcggc gtcgagcccg aggaccgtga tggccgtcag cacccaggca acccgcgcga
      841 cccacaacca gcgacctcgc aacggccaag cgatgcctgg ggcggttcgc cggctcaacg
      901 gtacttccgc atccttgacc tctcctcacc gtcgcttagc cacagtgaaa catgggttgg
      961 ctctaatgtc cgcctgacga cggccacgag ctcgcccacc aggttctcga ggtccacctc
     1021 ctctcgcaac cggagcgaga ggcgatccag ggtcctggcg gcgtcgtact ttctgcggta
     1081 gaagcggcgg tcgataaagg cctggatgcg ccggcgtaac ggggtgaaca gcgccgctat
     1141 ggccagcgtg gagaccacca cggccaactg tggctgctgc tccctgccgg tgagggtacc
     1201 caggacagcc tgggtaccga ccactccccc gaagtacaca gccgccagca tgaccgtcag
     1261 cgaaccgtac acgagggcgc ggttgatgat cacgtctata tcgtagaggc ggtatttcag
     1321 gacggcgaag cctatggcga tggggatccc ggcgtagctc atcaggacga ggttcgggat
     1381 cagcgcgtac aagaggtttc tgggtacctg gggggagaaa agttccggca agaagaagat
     1441 gccggtgatt acggcgctca gataagatat ccccacaacc gaggcggcga aggccagcca
     1501 tttgatctgc tcgcgtacgt ccgcgccgga acgtcggtag cgtaacacca ggctcaccgc
     1561 cgagaccacg atgcacgtgg gaagcagcaa cacgccgaaa acggccacgc tctctagcca
     1621 gggatattgc tccaacccga atggattgcg gaccccgggg tgatcctcta gttgcccggg
     1681 aacgaaaacg aacaggatgg ggatcagaac tatcaccgcc ccggcgaaga gggcaaacgg
     1741 acgccacctt ctcgacggca gcctgccgtc aggaaacagc aggaccatgt agacacccag
     1801 cagtcccacg ggcagcgtcc aggatccctg cagtagcgcg tccagtgtca ccgaagacgt
     1861 tacggtaccg gtcctggcaa gctcgtaggc tttggacgcg tccccctggg cgaaggacat
     1921 ccagaaaagt ccggagacga ggcagatcca accaatcggg ttcccgggac gcttggaggc
     1981 gatcagggcg ccgacgatgg gaaaggccag taagggaagg tagaccagca gctcgccgaa
     2041 gccgcccgtg gagacctgct tgacgggctc gtcgacgccc agagtcaaga acgcgaacac
     2101 gaaggcagat gcgtacagag caaacgagat gccggcaagc acccaggcga accagagggc
     2161 gccgcgacgg ctcatcgccg gcctttattc tggtacgccc cagccagacc gtcgtgggca
     2221 cggctcgtgt tttggtccgg gaccacctcc acgccccgtt acgccaggta gcgtgagact
     2281 actctggccg cgaggtagcc gctcatgccg tgcacgccgc cgcctggtgg cgtcgaggag
     2341 gagcagatgt agaggccacg ggccggggtt gagtaggggt tcgggcgggg catgggcctg
     2401 gtgaagagtt gcctcaggtc catgtagcca ccgttgatgt cgccgcccac gaggtttggg
     2461 ttccaccgct ctagctccgt cgaaccagcc gcacgcttgg cgagtacgcg ctccccgaag
     2521 ccgggggcga accgctctat ctgggcctct atcctccccg tcatgtcgaa ggtg
//
LOCUS       DBNGVD010000571         3610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3613707, whole genome shotgun sequence.
ACCESSION   DBNGVD010000571 DBNGVD010000000
VERSION     DBNGVD010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3613707"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1488)
                     /locus_tag="AC16CM_15345"
     CDS             complement(46..1488)
                     /locus_tag="AC16CM_15345"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3141255.1"
                     /translation="MGRFDTVVLGGQTVLPVRGRVRADVGIRDGRIAAVGDGFSPEDA
                     DEVVDASGKIVVPGAVDPHFHLGIYRDITEDTRSETLSSLAGGVTSVISYFRTGSHYL
                     EKSGPYAEIFPEVLAATNGHSRVDFGYHLAPMMRDQIGEIENLVSKEGVTSFKYYMFY
                     KGLDLSGASSDASGYTMSENYDLGHLFEIMEQVSKAQAERTDGSRVSLSIHCEQPELI
                     RVFSERVKNEGLLSGLEAYSASRPTLTEHLAVAEVAVLAEHTRCPVNLLHLSSAEAFE
                     AALEMKALNPYLDVRLETTLHHLALTYETYNDQRGKVNPPIRAQSDVDALWRGVARGE
                     IDWVCSDHACCSEEHKEGDLWQALPGFGGTALIYPFILSEGPKHGLSLERCVDLVATN
                     PARAYGLAPRKGSIAVGADADLAIVDMDETHPVTTEHLLSAQEYTPFEGMGLTGWPVR
                     TILRGETVFANGEPVGEPSGTYLKRPLDIP"
     gene            1624..2814
                     /locus_tag="AC16CM_15350"
     CDS             1624..2814
                     /locus_tag="AC16CM_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3141256.1"
                     /translation="MDGGQALGGIRVIDAATLFAGPLAATVLGDFGAEVVKVEHPRGD
                     PSRSHGYAKDGVGLWWKMLGRNKKCVTLNLGKPEGQEIFERLAADADVVIESFRPGTL
                     ERWNLGYERLSGINPGLVLARVTGFGQFGPYSNRPGFGTLAESMSGFAHVTGQPDGPP
                     TLPPFGLADGIAALATATAVLTALRARDVTGRGQVVDLAIIEPIFTVLGPQPTVYDQL
                     GVVQQRSGNRSVNNAPRNTYETSDGHWVAVSTSAQSIAERVMRLVGRPEYIDEPWFQK
                     GSGRAEHADELDEAVGAWISRRTRDEVMGAFEEAGAAVAPIYDIEGIMEDPQYRALES
                     IITVEDPEIGPLKMQNTLFRLSETPGGVKWSGPTIGEHNEEVYGGLGIDKQELDELSG
                     RGIL"
     gene            2811..>3610
                     /locus_tag="AC16CM_15355"
     CDS             2811..>3610
                     /locus_tag="AC16CM_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3141257.1"
                     /translation="MTPPARSYLYVPGDDERKIWKALAGEADAVVIDLEDAVAPNRKE
                     EARATAARVLGSEPGKPVFVRVNAVGTDLFAADIDAVAAGHLAGLRLPKVESVEGVRL
                     VAEALEGLQSGAVIQCLIESALGLERAFEIATSHERVSGTSLGEADLAADLGLSDEVG
                     LLYARSRVVVASRAAGLAPPVTSVYTKVRDLEGLRRSTETAKRIGFFGRSAIHPSQVP
                     VMNEVFTPQEHEVAEARELLGRLEDETEAGTGAFVLDDGRFVDRAVVES"
BASE COUNT          611 a         1191 c         1241 g          567 t
ORIGIN      
        1 cacgaggggg gcttcatcgg taccgctgga gcttctcaca gaactttacg gaatatcgag
       61 cgggcgcttg aggtaggttc cggagggttc acccacgggc tcgccgttgg cgaagacggt
      121 ctctccgcgc aggatggtac gcacgggcca gccggtcagc cccatgccct cgaacggcgt
      181 gtactcctgg gccgagagga gatgctccgt ggttaccggg tgggtctcat ccatgtccac
      241 gatggccagg tcggcgtcgg cgccgacggc gatggatccc ttgcggggcg ccaggccgta
      301 ggcacgggcg gggttcgtcg cgacgaggtc cacgcagcgc tccagggaga ggccgtgctt
      361 cggaccctcc gagaggatga acgggtagat cagtgcggtc ccgccgaagc cggggagggc
      421 ctgccacagg tccccctcct tgtgctcctc ggagcagcag gcgtgatccg agcacaccca
      481 gtcgatctca ccccgcgcaa ccccgcgcca gagcgcatcc acatccgact gggcccttat
      541 gggggggttg accttgccac gctggtcgtt gtaggtctcg taggtgagcg ccaggtgatg
      601 gagcgtcgtc tccagccgca cgtcgaggta agggttcagc gccttcattt ccagcgccgc
      661 ctcgaaggcc tccgcgctgg agaggtgcag caggtttacg gggcagcggg tgtgctccgc
      721 gagcaccgcg acctcggcga cggccagatg ctcggtcagg gtcgggcggg aggcgctgta
      781 ggcctcgagg ccggagagca acccttcgtt cttcaccctc tcggagaaga cccggatcag
      841 ctccggttgc tcgcagtgga tagagagcga gacccgcgag ccgtccgtcc tctcggcctg
      901 cgccttcgac acctgctcca tgatctcgaa gaggtgcccg aggtcgtagt tctcgctcat
      961 ggtgtagccg ctggcgtcgc tggaggcgcc ggagaggtcg aggcctttgt agaacatgta
     1021 gtacttgaac gaggtaaccc cctccttgct gacgaggttc tcgatctcac cgatctggtc
     1081 cctcatcatc ggtgcaaggt ggtagccgaa gtcaacgcgc gagtgcccgt tggtggcggc
     1141 aagtacctcg gggaagatct ccgcgtaggg tccgctcttc tccaggtagt ggctccccgt
     1201 gcggaagtag ctgatgacgc tcgtgacgcc gccggcgagc gaggagagcg tctcgctcct
     1261 ggtgtcctcc gtgatgtcgc ggtagatgcc gaggtggaag tggggatcga ccgcgccggg
     1321 gaccacgatc tttcccgaag cgtcgacgac ctcgtcggca tcttcagggg agaaaccatc
     1381 gccgaccgcc gcgatgcgcc cgtccctgat ccccacgtcg gcccgtaccc ggccccgtac
     1441 gggcagaacc gtctgcccgc caagcacgac ggtatcgaat cggcccatcg ccacccctct
     1501 ccccgaagaa cgtcgttcgc caacatagca cggtcctcgc gtcccggtca aaaccttgcg
     1561 acgggacggc acatgagcaa agatgaatgc ctgttgaggg gtccggtgac tggagggggc
     1621 ggtttggacg gggggcaggc gctagggggc atcagggtca tcgacgcggc gacgctgttc
     1681 gccgggccgc tcgccgcgac cgtgctcggg gatttcgggg cggaggtagt caaggtcgaa
     1741 catcccaggg gggacccctc gcgctcccac ggctacgcca aagacggcgt cggcctgtgg
     1801 tggaagatgc tcgggcgcaa caagaagtgc gtgaccctga acctgggcaa gccggaggga
     1861 caagagatct tcgagcgtct cgcggccgac gccgacgtcg tgatcgaaag cttcaggccg
     1921 ggcaccctgg agcggtggaa cctcggctac gaacgcctga gcggtatcaa ccccggcctc
     1981 gtactcgccc gcgtaacggg cttcggccag ttcgggccgt acagtaaccg ccccggcttc
     2041 gggacgcttg ccgagtccat gagcggcttc gcccacgtga cgggccagcc cgacgggccg
     2101 ccgacgctgc cgcctttcgg gctcgccgac ggcatagcgg cgctcgccac cgccaccgcg
     2161 gtcctgaccg ctttgagggc ccgcgacgtg accgggcgcg ggcaggtcgt ggacctggcg
     2221 atcatcgagc ccatctttac cgtgctcggc ccccagccta cggtctacga ccagctgggt
     2281 gtcgtacagc agcgcagcgg caaccgctcc gtgaacaacg ccccccgcaa cacctacgag
     2341 accagcgacg gccactgggt tgccgtctcc accagcgccc agagcatcgc cgagcgcgtg
     2401 atgcgcctcg tcggccgccc cgagtacatc gacgagccct ggttccagaa gggctccgga
     2461 cgggccgagc acgccgacga actggacgag gcggtggggg cctggatctc caggcgcaca
     2521 cgcgatgagg tcatgggggc tttcgaggag gcgggggcgg ccgtggcccc catctacgac
     2581 atagaaggca tcatggaaga cccccagtac agggcgctcg aatctatcat caccgtagag
     2641 gaccccgaga ttggcccctt gaagatgcag aacaccctct tcaggctctc ggagacgccg
     2701 gggggcgtca aatggtcggg ccccaccatc ggggagcaca acgaagaggt ctacggcggg
     2761 ctcggcatcg acaaacagga actcgacgag ctctccggca ggggcatcct gtgacccctc
     2821 cggcccggag ctacctctac gttccgggcg acgacgagcg caagatctgg aaggcgctag
     2881 ccggcgaggc cgacgccgtc gtcatcgacc tggaggacgc cgtggccccc aaccgcaagg
     2941 aagaggcccg cgctacggcc gcccgcgtcc tcgggtcgga gccgggcaaa cccgtcttcg
     3001 tgagggtgaa cgcggtcggc accgatctct tcgcggcgga catagacgcg gtggccgcgg
     3061 gccatctcgc cggcctcagg ctacccaagg tggagtccgt ggagggcgtg aggctggtgg
     3121 ccgaggcgct cgaagggttg cagagcgggg ctgttataca gtgcctgatc gaatccgccc
     3181 tcggcctgga gcgcgccttc gagatcgcca cctcccacga gcgggtctcg gggacgagcc
     3241 ttggcgaagc cgacctcgcc gccgacctcg gcctctcaga cgaggtgggg ctgctctacg
     3301 cgcgctcgcg cgtggtggtg gcgtcccgag ccgcgggcct ggcccctccc gtgacgagcg
     3361 tatacacgaa ggtacgcgac ctcgaaggcc tgcgccgctc cactgaaacg gcaaagcgaa
     3421 tcggcttctt cgggcgctcg gccatacacc cctctcaggt acccgtcatg aacgaggtct
     3481 tcacaccaca agagcacgag gtcgccgaag cccgggaact gttggggcgc ctcgaggatg
     3541 agacggaggc cggcacgggc gccttcgtgc tcgacgacgg caggttcgtg gacagggccg
     3601 tggtcgaatc
//
LOCUS       DBNGVD010000572         5152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_881913, whole genome shotgun sequence.
ACCESSION   DBNGVD010000572 DBNGVD010000000
VERSION     DBNGVD010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_881913"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16CM_15360"
     CDS             complement(<1..707)
                     /locus_tag="AC16CM_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3141258.1"
                     /translation="MEAVIVRAVVVDPVSKANLSLAEVGEPEPGPSESIVRVAAVSLN
                     RGEVRRAEAAEPGFRPGWDLAGTVERSAPGGPAEGTSVVGFLPSGAWGERVAVPTNAL
                     AELPEGVSFGEAATLPVAGLTALYTLEMGGNLLGRDVLVTGASGGAGQFALQLARMSG
                     AQVVALVRRAEHEELAREAGAHEVAVDEGGAAAGEYGPYHLILESVGGEVLGNALSML
                     APEGTCVSFGVSGGPEVT"
     gene            760..1782
                     /gene="corA"
                     /locus_tag="AC16CM_15365"
     CDS             760..1782
                     /gene="corA"
                     /locus_tag="AC16CM_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3141259.1"
                     /translation="MQPTTTRNLPENVDLMVLDASGKLREDIRLEELPDFLADRDSLV
                     WCDIFSLQGGQNGPYGRILSEVFGFDALTIEDCFTKSHLPKVDLYEDYLFMALFSFHL
                     SEKTSRVQTVEVDMYVGENYVVCVHQRPLRELDRVRRRLLAGNEFVASSPANVAHTVF
                     DAVVDEYLPIMNRLSAMVDGIEDDLLSEGEEASDQILGTLFHLKHELTALRRLAVPLR
                     DVVGVLMRPTTGLIPDESRAYYDDVRDHLLRVIDMIDTMRDYLAGSLDIYTTQQTQRI
                     NQSMQRLTAISLIFLPLTFITGIYGMNFAYMPETHLRLGFYAVLTLCALVGGGMLYYL
                     RRKRML"
     gene            1866..3152
                     /locus_tag="AC16CM_15370"
     CDS             1866..3152
                     /locus_tag="AC16CM_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3141260.1"
                     /translation="MRPRETKGREGGLRQRLRDRPRLRPRFYGYLTEEKETLRQGFAA
                     LFLSSTGELIAGIVLAGIAGTLKELVGLAVLIPAAIGMRGAIFGAMGSRLSTSIQTGL
                     FELNLRRGVLAENVQAAAVLSLVSGVFLAVLARVLCGILGVETELTVVDYIVISTAGG
                     ILAGVLLLGITVLVARLSVARGWDMDNIAAPLLTAAGDILTLPSLVVATYLIGIPVFS
                     SALGAALVIGAVAAGFLGLRLGAANLKRILAESFPILALTGTVTILVGVALQDREEQF
                     TTLLALSILLPAFLQEGGALGGILSSRLSSKIHLGLIEPRGIPQFTAFRDFTLTYIFA
                     AGVYVFIGAASHFIALVLFPPGSSPGLLAMIGISALAGLIATTTAVLAAYYGSTISYR
                     LGLDPDTYGIPIITAAVDLLGFMSLIIALIVFGLAG"
     gene            3156..4355
                     /locus_tag="AC16CM_15375"
     CDS             3156..4355
                     /locus_tag="AC16CM_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3141261.1"
                     /translation="MRETRPRNLKEMLVEAKDTSELMVDLSYAAIFYNSETLSEEVSR
                     LEEMLNGLVYDMRALAILAARSPADAEQMAGILGVVQDIEKIGNAAVDIAKIVVKRLG
                     IPPELLHDIPEAEEIPSRVRIQQESFLDGRSLGDVDLPVETGMRPIAIRSDDEWHFDP
                     EPTYVLRSGDVLFLQGPPEGVVEVRELAGAPPLEAAPETGPPPSQLSEIDHAVDILIE
                     MKNLSEVAVGLAYSALLYDDAGLAREVIAIEDEMDEMRYRLERWVLLAAPHVEDSSRL
                     RGLLHLATASETIADCAMEMVWVVEKGEEVHPVLSAAIGESDEIVLKLTVSPDSPADG
                     KSLESLELETETGMYALAVNRGGRWTYRPRDTFRLKGGDSLLFTGAPEGLEPLAELFG
                     QELEGPG"
     gene            4448..>5152
                     /locus_tag="AC16CM_15380"
     CDS             4448..>5152
                     /locus_tag="AC16CM_15380"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR3141262.1"
                     /translation="MSAGLLLLSANLPPELREGSNDLTLYRLAGESILRGEVPYRDFF
                     IEYPPGSLLAFIPPAISSAGRAGFIDLFSLEMALALAATLLLVALAARRLRGPRGWIL
                     PAATFVPAALVLYPVAVTRYDAIVALSLGLTVFFASLGGRYLSLAYASLGFGAAAKFV
                     PALAVLPLTLTRRGAARGLAVFLIVAALCFVPFGLLGGRGLVQSFAYQSGRGLQVESL
                     AASVLMVLRFADGIVFQ"
BASE COUNT          825 a         1785 c         1620 g          922 t
ORIGIN      
        1 gtgacctctg gccctcctga gacgccgaag gagacgcagg tgccttctgg ggccagcatg
       61 gagagggcgt tgccaaggac ctcgccgccc acggactcca ggatcaggtg gtacggtccg
      121 tactcgccgg ccgcggcgcc accctcgtcc acggccacct cgtgcgcgcc ggcttctcgt
      181 gccagctctt cgtgctccgc cctacgtacg agggcaacga cctgcgcccc tgacatcctg
      241 gcgagctgca gggcgaactg gcccgctccc ccggacgcgc ccgtgaccag gacgtctctg
      301 ccgagcagat tcccacccat ctccagggtg tacagggcgg tcaggccagc caccggcaac
      361 gtggccgcct ccccgaacga gacgccttcg ggcaactcgg ccagcgcgtt cgtcgggacg
      421 gcgacgcgct caccccatgc cccggatggt aggaacccca cgacactggt gccctcggcg
      481 gggccgcctg gcgcagagcg ttccacggta ccagccaggt cccacccggg gcgaaagcca
      541 ggttctgccg cctccgcgcg gcgcacctcg ccgcggttca aggagaccgc cgcaaccctc
      601 acgatcgact ccgagggccc cggctcaggt tcccccactt cggccaggga gaggttggcc
      661 ttcgatacgg gatctaccac cacggcacgc acgattaccg cctccatccg ttgctggtca
      721 aaccgcagtc tactacgacc ttcgctgtat aatcctggca tgcagcccac cactaccaga
      781 aaccttccgg agaacgtcga tctgatggtt ctcgacgcat cgggcaagct gcgcgaggat
      841 atcaggttgg aggaactacc cgattttctg gccgacagag actcgctggt ctggtgcgac
      901 atcttcagcc tccagggcgg gcagaacggg ccttacgggc gcatcctgag cgaggtgttc
      961 gggttcgacg cgctcaccat cgaggactgc tttacgaaga gccatctccc aaaggtggac
     1021 ctctacgagg actacctgtt catggcgctg ttctcgtttc acctctcgga gaagaccagc
     1081 cgcgtccaga ccgtcgaggt ggacatgtac gtcggggaga actacgtggt ctgcgtgcac
     1141 cagcgtcctt tgcgggagct agaccgggta aggcgcaggc tgcttgccgg caacgaattc
     1201 gtcgcctcct ccccagccaa cgtggcccac accgtcttcg acgccgtggt cgacgagtac
     1261 ctaccgatca tgaaccggct ctcggccatg gtcgacggca tagaggatga cctcctcagc
     1321 gagggagaag aggcctcgga tcagatcctc ggaaccctgt tccacctcaa gcacgagctg
     1381 acggccctga ggcggctggc ggtgcccctg cgggacgtgg ttggcgtgct catgcggccg
     1441 acgacagggc tcatccccga cgagagccgg gcctactacg acgacgttcg ggaccacctg
     1501 ctcagggtga tagacatgat cgataccatg cgcgactacc tcgccggctc gcttgatatc
     1561 tacaccaccc agcagaccca gcgcatcaac cagagcatgc agcggctcac ggcgatctcg
     1621 ctgatcttcc tgccgctgac gttcatcacg ggcatctacg gcatgaactt cgcgtacatg
     1681 cccgagacgc acctgcgcct gggcttctac gccgtcctga ccctctgcgc cctggtcggt
     1741 ggcgggatgc tctactacct gaggcgcaag aggatgctct agcttcccgc cagaaccctg
     1801 gacgccgtca ccggcggcgc tttacaagca actcgcgacc ggctatacca ccccatgtga
     1861 gcgagatgcg accacgggag accaaaggca gggagggtgg cctgcgccag aggctgcgcg
     1921 acaggccccg gcttcgaccc aggttctacg ggtaccttac ggaggaaaag gagaccctca
     1981 ggcaggggtt cgccgcgctg ttcctgtcct cgaccgggga gctaatcgcc ggcatcgtgc
     2041 tcgccggcat cgcgggtacc ctcaaggagt tggtcggact tgcggtgctc atcccggcgg
     2101 ccatcgggat gcgcggcgcc atcttcggcg ctatggggag caggctctcg acctctatac
     2161 agaccggcct cttcgagctc aacctcaggc ggggcgtgct cgccgagaac gtgcaggccg
     2221 ccgccgtgct ctcgctcgtc tctggggtgt tcctggccgt cctggcccgg gtattgtgcg
     2281 gcatcctcgg cgtcgagacg gagctcacgg tcgtcgacta catagtgatc tccacggccg
     2341 gcgggatcct cgctggcgtg ctgctgcttg ggatcacggt cctggtagcc cgattgtcgg
     2401 tggcgcgcgg ctgggacatg gataacatag cagcccccct gctgaccgcg gccggcgaca
     2461 tactaacgtt accctccctc gtggttgcca cgtacctgat agggataccg gtcttctcgt
     2521 cggccctggg cgctgcgctg gtcatcgggg cggtggcggc cggctttctc ggcctcaggc
     2581 tcggggccgc gaacctgaag cgaatcctgg ccgagtcgtt cccgattctg gccctcacgg
     2641 ggaccgtaac catcctggtt ggcgtggcgc tgcaggaccg cgaggagcag ttcacgacgc
     2701 ttctggccct gagcatcctc ctgcccgcct tcttgcaaga gggtggggcg ctcggcggaa
     2761 tattgtccag ccggctctcg tccaagatcc acctcggcct catagagccg cgggggatac
     2821 cgcaatttac ggccttcagg gacttcaccc tcacctacat cttcgccgcc ggcgtctacg
     2881 tgttcatcgg ggcggcgtcg cacttcatag cccttgtcct gtttccaccg ggttccagcc
     2941 cggggctgct cgccatgatc gggatcagcg ccctggccgg cctgatcgcc acgaccacgg
     3001 cggtactcgc cgcgtactac ggttcgacga tcagctaccg gctcggcctc gaccccgaca
     3061 cctacggcat cccgataatc acggcggcgg tggacctgct ggggttcatg agccttataa
     3121 tagcccttat cgttttcgga ttggcggggt aagagatgcg cgagacgcga ccacgaaacc
     3181 tcaaggagat gctcgtcgag gcgaaggata cctcggagct gatggtggac ctctcctacg
     3241 cggcgatctt ctacaactcc gagacgctct cggaggaggt atcccgcctg gaggagatgc
     3301 taaacggcct cgtctacgat atgcgcgccc tcgccatcct ggcggcgaga agcccggccg
     3361 acgccgagca gatggccggc atcctcggcg tcgtgcagga catcgagaag atcggcaacg
     3421 ccgccgtcga tatcgcgaag atcgtggtga agcgcctggg catcccaccc gaactcctgc
     3481 acgacattcc ggaggccgag gagatcccct cccgcgtccg catacagcag gagagtttcc
     3541 tggacggccg ttcccttggc gacgtcgacc tgcccgtgga gaccgggatg cgccccatcg
     3601 ccatccgctc cgacgacgag tggcacttcg accccgagcc cacgtacgtg ctgcgctccg
     3661 gcgacgtact cttcctgcag ggcccgcccg agggcgtcgt cgaggtcagg gagctggcgg
     3721 gggcgccccc gctcgaagcc gcccccgaga ccggtccgcc accgagccag ctctccgaga
     3781 tcgaccacgc cgtggacatc ctgatcgaga tgaagaacct ctcggaggtg gccgtgggcc
     3841 tggcctactc ggccctgctc tacgacgacg ccgggctggc gcgcgaggtt atagctatcg
     3901 aggatgagat ggacgagatg cgctaccgtc tggagcgctg ggtgctcctc gcggccccgc
     3961 acgtcgagga ctcttcccgg ctccggggcc tgcttcacct ggccaccgcc tccgagacca
     4021 tagcggactg cgccatggag atggtgtggg tcgtggagaa gggcgaggag gtccatcccg
     4081 tcctctccgc cgccatcggc gagtcggacg agatcgtcct gaagctgacc gtctcgcccg
     4141 actcgccagc cgacggcaaa tcgctcgagt cgctggagct tgagaccgag acgggtatgt
     4201 acgccctcgc cgtgaaccgc ggcggacgct ggacctacag gccccgagac accttcaggc
     4261 tcaagggcgg cgactcactg ctcttcaccg gggccccaga aggcctcgaa cccctggccg
     4321 agcttttcgg acaggaactg gaaggcccgg ggtaatccag ggagaccggg aaaacccggt
     4381 agcgcgggga cgggcaccac gtcccacccc ctggttctgg accgcgattg tctccctcgc
     4441 ggcctcgatg tcagcgggcc tgctcctcct ctcggccaac ctgccgcccg agctgaggga
     4501 aggatccaac gacctcaccc tgtaccgtct ggcaggagag tccatcctgc gtggcgaggt
     4561 cccctacagg gacttcttta tcgagtatcc accggggagc ctcctcgcct tcatcccccc
     4621 cgccatctcg agcgcgggcc gggcaggctt tatcgatctc ttctccctgg agatggcgct
     4681 cgcgctcgcc gcaacgctcc tcctcgtggc gctagctgcg cgcaggctcc gtgggccacg
     4741 cggctggata ctgcctgcgg cgacgtttgt gcctgccgcg ctcgtactgt atcccgtggc
     4801 cgtcacccgc tacgacgcca tcgtcgcgct ctcgctcggg ttaacggtgt tctttgcttc
     4861 tctggggggt cggtatcttt cgctcgctta cgcctcgctc ggtttcggcg cggccgccaa
     4921 gttcgtgccg gccctcgcag tactaccgct gacgctcacg cggcgcggag ccgcgcgggg
     4981 cctggccgtc ttcctcatcg tggcggcgct ctgtttcgtg cccttcggcc tcctcggtgg
     5041 ccgggggctc gtgcagagct tcgcctacca gtcagggcgg gggttgcagg tggagagcct
     5101 tgcggcgtcg gtcttgatgg tcctccgctt cgcggacggc atagtcttcc ag
//
LOCUS       DBNGVD010000573        22557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4525356, whole genome shotgun sequence.
ACCESSION   DBNGVD010000573 DBNGVD010000000
VERSION     DBNGVD010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22557
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4525356"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..463)
                     /locus_tag="AC16CM_15385"
     CDS             complement(125..463)
                     /locus_tag="AC16CM_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141263.1"
                     /translation="MTESNGRVEIERRLIERSLQDEDFRRRLLADPRATLEQELGMPL
                     PEGVTIQAVEETADTIYLVLPGRSVDDRVGELSDLDLETVAGGGWTDSDCASGDTNQA
                     HTCTCGCTPP"
     gene            complement(574..888)
                     /locus_tag="AC16CM_15390"
     CDS             complement(574..888)
                     /locus_tag="AC16CM_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141264.1"
                     /translation="MSEASGRMEIERRIAQRTLNDESFRKRLLEDPRATMERELGAPL
                     PEEMRVEVVEETADTIYLVLPSASSAGAENDELSDEELEAVAGGWEPNTCMATESTAG
                     CA"
     gene            complement(1035..1868)
                     /locus_tag="AC16CM_15395"
     CDS             complement(1035..1868)
                     /locus_tag="AC16CM_15395"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3141265.1"
                     /translation="MVEADIVSLRSQRQGVNRGHWWGEKEVPSSLPGRRFHLIGVGLP
                     KTGTTSLALLFEPFRWGYEFLFAETVVHLVAWRNGRLSRDGMRRWLARRAEAGTLELD
                     SASFNHYYLDILVELHPEARFVYTQRDAVSWADSFLNMMLQYGAFLDGRPWPDWHLAL
                     GRLMAPSLDPGQFASAEHLRATLRPLAVDLLRFHARQTARIEAALPPERSLVIPTDGI
                     STSLDALAAFAGVPKAALNTAKPHGNRGTRKIPLVAALPDLPHLLEQNGLGKDPAASG
                     Y"
     gene            2367..3161
                     /locus_tag="AC16CM_15400"
     CDS             2367..3161
                     /locus_tag="AC16CM_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3141266.1"
                     /translation="MDKMLSPDELDELMRVTDPRGWLALIALLALVIAAVGWSIFASV
                     PVEVGGDQGVLIHSGARHGVASQVSGLVTQVEVKPKDQVKEGQVIARVQTDSGEKADV
                     VTLYSGRAEEVLVQKGMVLNRGQQVAVVEQGSKPLEAVIFAPTEQGKQLEKGMRVQIS
                     PTTAKVEEYGFMEGRVTSVSEFPVTRGEMFAVLANQSLVDTLRTSEDQLQVDVKLQSD
                     PSTPSGFSWSSSNGPPFSISHGTPVTATFVLGKQHPINLVFPSSNE"
     gene            3256..5418
                     /locus_tag="AC16CM_15405"
     CDS             3256..5418
                     /locus_tag="AC16CM_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HIR3141267.1"
                     /translation="MLQMEAVECGAAALGIILGYYGRVVPLEELRVACGVSRDGSKAS
                     NVVKAARGYGLIAKGYRKEPDELRTLAPPFIAFWNFSHFLVIEGFGRRGVYVSDPANG
                     RRIATYDEFDRAYTGVVLTFEPGPEFRKGGARPSLLAAMRTRLRGSEWALAFVVLASL
                     ALVVPGIVIPIFSQIFVDQYLVQGSRDIILPLLGGMALTALLRAGLTWLQQHYLLGLQ
                     TKLSISMSSKLFWHALRLPMEFYGQRYAGEVGSRVTINDRIAQLLSGQVATTALNLIT
                     VLFYVVVMLQYDVPLTLIGIVFAAFNIAVVRFVSRKRIDANQRLLLERGKLTGVSMGG
                     LQSIETLKATGSESDFFVHWAGLEAKLLEAQQELGARTQLVSAVPPMLTALTTAAVLG
                     VGALRVMDGDLTIGMLVAFQSLITSFMTPFGQMVALGTTLQETRGDLNRVDDVLRYEP
                     DPQVPAKIGAPEEISPDLPSELSGYLELKNLTFGYSRLEAPLIEGFSLTLQPGSRVAL
                     VGASGSGKSTVAKLVSGLYPPWEGEILFDGQQRTTVPHGLLTSSLTVVDQDISLFEGT
                     VRENLTLWDDSIAQGSLVRAARDACIHDDIAARQGGYESKVDEDGGNFSGGQRQRLEI
                     ARALVSDPTIVILDEATSALDPTTEGQIDDNLRRRGCTCLIIAHRLSTIRDCDEIIVL
                     DRGKVVQRGTHEQMRDVDGPYARLTGALEKETRREGPT"
     gene            5418..8351
                     /locus_tag="AC16CM_15410"
     CDS             5418..8351
                     /locus_tag="AC16CM_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HIR3141268.1"
                     /translation="MPPEEMMPEGQQWLLRLGDQKGRRIEAGGNAPISLDDPDSVFVV
                     SSGKVDVFSTQADVGQIMGPRRHLCRVTAGQVLFGIDLGHAPEEFGILAVGVADTVLI
                     GLRRSELAESTKGTVHAGLVGALLEGWLEKLSSAVAVQHHTPRESRTLAPGQKISIDA
                     DETVRAERNTVWVRHLDGSSRFLGDADLPPVGGDGLFPLSDPAWLASGGGTALEGVGT
                     VDYVAGDASWSGLDSFHAVVVSALDLETRLSFSERRELEDERESADVELARSAVNQLG
                     SVLEKDAFTEDDDQETPTGDPLLAACQVIGRRLGVELRRPAWSDGTARDPLEEIARAS
                     RVRIRRVALKGDWWRRDSGPLLCFREDESPVALLQRGTSAYTIVDPARGTSDRVTGET
                     AAELADFAYTFYPPFPDRPLGAWDLLKYSLRNLRGDLLRVSLMGVAGGLLGALVPIVT
                     KQIVDIAIPQAEGGFAVALALGLAVAAVSAMLFEMTRQFAVLRIETKLDSGVQAAVWD
                     RLLSLPAPFFREYTAGDLAVRAMGINTIRQMVTGATISSLLSGVFSIFSFAVIFYYSA
                     ELGLIAALMAAVIVVVTAAGGFVQLRYQRRETEMLGKVSSLVLQMLTGIAKLRVAGAE
                     MRAFSVWSGQYSIQKANAVRAQAAANALTVFNSISGVLTYMVIFAVVAYVLDGGVSTG
                     EFVAFNTAFAQFLFASAGITTAFTTILQAAPFYERAKPILRTLPEVDTEKPDPGVLRG
                     RVEINQLSFRYDPDGPLVLDNVSLRADPGEFVAIVGPSGAGKSSLFRQLLGFEDPESG
                     SVYYDDHDLTGLNVQAVRRQLGVVLQNGNMMSGTIFQNIAGVSDLTMDQAWEAARMAG
                     LDKDIEMMPMGMFTFLGQGGSSVSGGQRQRLMIARAIATKPRVLLFDEATSALDNRTQ
                     AIVSSSLESLKATRIVIAHRLSTIMNADRIYVLEAGHVAQSGTYDELMEQEGPFRDLA
                     MRQIA"
     gene            8384..9535
                     /locus_tag="AC16CM_15415"
     CDS             8384..9535
                     /locus_tag="AC16CM_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141269.1"
                     /translation="MMDSFEGYLGPVAAGVAPELISPSAFSDIRSVAGVLPGTLAYNT
                     FGFECRLGEELPRADFLVLANAYCGRDSLAGLHPTSPLSPRLSADPIWRRVAAFASRW
                     ADASSLLYNTADNVWLEFDVEGPAPDVPVPSLFFGPRTDGRDGGAALRPDVEGYRATI
                     VEALRPLSRGEPPAGMLETMSGCLRALAPDDLVFQVGLMMSRGAETVRLCIRPGSVER
                     ITEYLAEVGWTGSGEDLRAILEPVERLVDYVCLDIDVGDTVHPKIGLECYIRGPGQAR
                     IRARWEAFSGFLLESGLCTPDKREALLAYPGYIDENAEGVSWPVPLRRASLLLGGRSL
                     SMFVRSIHHVKIVCQQGRPTEAKAYLAANHHWHTRAEAENTAPREPMRT"
     gene            9635..9982
                     /locus_tag="AC16CM_15420"
     CDS             9635..9982
                     /locus_tag="AC16CM_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141270.1"
                     /translation="MNEATGRTEMERRLIERSLEDETFRQQLIDDPRNAVERELGTRL
                     PEELRVVAVEETADTIYLVLPPASQAGQPDEGLSDSDLDAVAGGGTWVDNTCGGQTAC
                     GCAPTAVGRGVGC"
     gene            complement(10092..11237)
                     /locus_tag="AC16CM_15425"
     CDS             complement(10092..11237)
                     /locus_tag="AC16CM_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7005 family protein"
                     /protein_id="HIR3141271.1"
                     /translation="MMTPEQRDELLASYGATVSEVEELQAYDTNTFDRSGEGWPPVLP
                     LPAEPHVAAWVGYAREAAGTNAFVTLGRRLAQLRFPIEEGISQTEAYRAATLRGASTE
                     GMAEASGLVLTRPQELTLRVHQSPAGPIPVLIPGGREDFVSLVRALTMRNEPRPVPGS
                     MGACMVSGFNDWERIRQYRRRWEGENGSTSEAAWKEEFRRLVPRKALYQDRFLILSDG
                     PYSGVAAPEMGFTEDEWRRLSTSIRLEHECTHYFTLRVFGSMRNNILDELIADFMGIT
                     AAAGRYRSDWLLRFMGLESFPAYREGGRLQNYRGQPPLSDGAFRVLQALVKDASENLE
                     SSRAGRSLPGGPEERAAMLLALTGFRLEDLAGPGAVGRISESVNLLQ"
     gene            11377..12570
                     /locus_tag="AC16CM_15430"
     CDS             11377..12570
                     /locus_tag="AC16CM_15430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3141272.1"
                     /translation="MDDRYLMDDGREISFHRDSGGDHLVVPVHRAEKERLSECLRRLE
                     SGGGRLDYSLQDHGSSSTSLRVSAGGADLDNLWTRLFTRLHRYPYYEEVQTESFRNAA
                     EPVLSCVVLLAGNDRFVQRHLVPSILANSEGYPIEIVIVYNGQCLDLGPFRNLRVVRS
                     DFGWVSRAYNAGARAARGRYVALFHDDCMVVSPRWIGTSIRMLEAGYVAVTPEVRRKT
                     TTWGTELLIAKNVPLIMRRRDFVASGGYDEFYYAGYEDQDFTYTLLSGGGKIGHLDLP
                     FLHFNGMSTVVLAGGAAGLFRVLFGYNALGQDLIYELREESLRSLRSHPKIGLSEARD
                     TLYFVRKHKAYFAERQNEAVLEMGQAMDEYLAAHLAEYLFDPVLQDRQKFTEFYRSVL
                     GLPAE"
     gene            complement(12675..12995)
                     /locus_tag="AC16CM_15435"
     CDS             complement(12675..12995)
                     /locus_tag="AC16CM_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141273.1"
                     /translation="MSEAAGGRAEIEQRLVQRSVEDESFRQSLLADPRAVVEQELGTP
                     LPESMEIRAVEETADTIYLVLPPASAPDQTGGTLSDDELEAVAGGGGWGSDESQCICS
                     NFCH"
     gene            13319..14938
                     /locus_tag="AC16CM_15440"
     CDS             13319..14938
                     /locus_tag="AC16CM_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141274.1"
                     /translation="MVLFDLHTLRGMADDTSVRGMFYLPQSTDLDLHSHVILTSQGTL
                     AASRNGSRLVDPLSETVFPAVGKGTSLYERFSDRFDLFPSDDVDCLAIGEMAPFPPVL
                     LHLRAENGFGEAKAVFAREPSTLHYELLKAIGVEYLGGNPEGAHYVARFRNHLPTHIH
                     SGVLAHFARTSHCNMFFFRHGSIDRPLERGLISASTNRVSWAAGRAREAVVRMANEAE
                     GEDAVSLGFLLRALNHHTSAASIFDNIDTRTRLVSLLRDGEHDSLWPYEVGGPASSTA
                     SAIVLQGLYDPAGVEALEAFADGRGGYYSRLPADQRETASDPANSGDGHHHPDFATAC
                     MIRALRNGAGLDTRTGVEYLATRFEGRSGLCFANPYLVDWALASALENDDSAQELRNM
                     LTSEILASMNDDYSFGRFDVPLSSAFAICSLASLGFRSRTLRLAQLRLLDFMQPDGTF
                     PAGTPLFSVRADTREPGCGPRDVFLRADRHGTISTAAGTLALLAACSPEEGDPYAGGE
                     WGGGAHPRYGCRDHEDYIRRFVLPPDGEGLS"
     gene            15051..18338
                     /locus_tag="AC16CM_15445"
     CDS             15051..18338
                     /locus_tag="AC16CM_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="HIR3141275.1"
                     /translation="MRQIDVLSEQDLVEIVERASSVDERLGDDFVPAADGGSAEAVDR
                     RLEAWSQALGRGDEDRFRRRLAWDGLEPESVRPVLGPVRLREGVRLPEWSGLIAEVLR
                     LAPPERQDGAEEAWPEELSFLDAEDVLPFEEILAPFVLVARERLAGRAAAYGGLLSAG
                     ARTDLERTLLRSLSFASGELFLAEFEAMRAQEQSSWDRLFALAQDPEGRSLYRRFVRR
                     SGEGGMASLFRRYPVLARHLGTISQLWVEANEEFLGRLEEDLPELERLFGDERFGEVV
                     GLGSSLSDPHCGRRSVLALTFASGRKVVYKPKDMGTEAAYHRLLAWLNERGAPLPFKV
                     LKVLDRSTHGWVEFVEYLPCQDEDEARRYYERAGMLLCLFYALEGTDCHYENIIASGE
                     YPVLIDTETLMHHRARAVIPDDENAQTEAFERLGHSVLRTGLLPRWELSVDEGKAYHV
                     SGLGEAEEQEWPIRMPTWVRKNTDRMALEYDFVKLQMRENVPTLEGVPLSLQEYATPL
                     VDGFRRMYRFLLEHREALLTAPPLQELARQQVRFVFRATKVYGLILRNLLHRQYLRDG
                     ADRSIQLEQLGRAQVPPRDALDEPDERPIFWPVFAAERLAMETGDVPFFTARASSDAL
                     ILAPGQEVEGCLQEPSFELVSRVLEGLGDSDLEQQVAFIEGSLYAHLARETTASHPVV
                     EEPQEDFPAGPPEGEDFIAPALELAEAIKSRAIRTEDGSAAWIAPQYLVQAERYQLQV
                     MDFSLYGGTAGVALFLAAAEQFAPDSGYGELAQAALRPLRSARDRRPDRFAERMGIGG
                     ASGIGSVAYSLLHVGLLLDNPALLEEARRVADLITDDLIASDKVLDVIGGAAGAILSL
                     TALYGVMGDQQILERAIACGEHLLEMRTETGAGPRAWATSEQLRTTGFSHGTAGIAYA
                     LLRLYEHTRDERLLEAADEAIAYEDTMYSPENHNWADHVKSGESNYLWQWCRGAPGIG
                     LARVGGLDVLDTGRVREDIELAMQSTLQYGVRGVDHPCCGSMGLAEVLLSAGQRLARP
                     ELEASARTNAWRVLDKANRTGGFVLHPLLPRQVDNPGFFQGTAGIGYELLRMARPDLL
                     PSVLLWE"
     gene            complement(18471..18698)
                     /locus_tag="AC16CM_15450"
     CDS             complement(18471..18698)
                     /locus_tag="AC16CM_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141276.1"
                     /translation="MSEASNREQMERRLIEKSLEDESFRRRLVEDPRGAVEEELGARL
                     PEGVRVAAVEETADTIYLVLPPRAGTGQSDG"
     gene            19058..20179
                     /locus_tag="AC16CM_15455"
     CDS             19058..20179
                     /locus_tag="AC16CM_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141277.1"
                     /translation="MVETRVRALAALAELAGEASRTPLSLICQPPPPTSPFPYGDLVP
                     LGFLLRAANACGSNPSVRSSLEDLLRDKRQGLLWAYHSDTLVTCIDSALVLQGFRDQA
                     GVEALELFADGRGGYLPQLCAERPERDHMTITAHNRHWCQPEYGTTCLASALRAEAGL
                     ERKTSVEYLAEGFENRGGLYFANPYMADWALAWALQGATSATALRERLATDILASANE
                     DGSFGRYDVPLSTALAILALISLGLEGEPVTRARLRLADMQLPDGTWPASIPFYSAVK
                     MPHERLPGGLLARLMLGERRGQLFWLEGGAYTASLYVDGYRTISSALASLALTQPGPL
                     TGLDALPKQSRESCHPRYRCADHVQYIAGYALPPYLVPR"
     regulatory      20280..20438
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            20445..20735
                     /locus_tag="AC16CM_15460"
     CDS             20445..20735
                     /locus_tag="AC16CM_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141278.1"
                     /translation="MPAQQPFWEQLMFGREHGPRAEREEKVLRYIVHRLDEEDAHLSD
                     VLQETYVRRNCTQEMVDEIVRNPELIHSCREHLERVFDSGELDPRTRPQVSH"
     gene            20896..22179
                     /locus_tag="AC16CM_15465"
     CDS             20896..22179
                     /locus_tag="AC16CM_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR3141279.1"
                     /translation="MIQQDRAPEKNWAGNYEYRARKLHRPSTMGQAQEIIANAPRLRV
                     LGSRHSFNDIADSSELITLEALPEDVVVDHGASTVSLNAAMTYGELEKTLRAEGVALH
                     NLASLPHISVGGAVATATHGSGPENGNLATAVAGIELVTSAGEIVEFARGDADFEGLV
                     VGLGALGAVSRVTLDVEPAYEVRQRVYERMGWEAFYERFDEVTSGGYSVSVFTRWAEV
                     IDQVWVKSRVTDEPERVEDELFGAVAAVEDRHPIPGVDAGPSTPQMGVPGPWAERLPH
                     FRLGFTPSSGEEIQSEYLVPRRHAPGAIEAVRSLAGEIQPHLQMSEIRRVAADRLWMS
                     MNYGEDTVGLHFTWKRRPDEVGDCLVRIEAALAPFEARPHWGKMFAAEAEAIAPLYPR
                     LPDFVRLMERLDPRGAFRNPWLEARVLGESAPRHP"
     gene            22214..>22557
                     /locus_tag="AC16CM_15470"
     CDS             22214..>22557
                     /locus_tag="AC16CM_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3141280.1"
                     /translation="MSESVEHIPPLALMVRAPDEPGALHALTGVLLDHDANIAHVDIA
                     ERSDGLSTVYFELEDVADGAAEVLVRDLASLPIVQVVELAPSFLKVYGKRIIVVGGGA
                     QVGQVVVGAVAEA"
BASE COUNT         3865 a         7323 c         7458 g         3911 t
ORIGIN      
        1 gaggtagtat cacacgctat cttttctcgg gcaaggtatg ctccagagcc ccggcagcgc
       61 ctttgaccgc cgcatcaccg gcggctgccg gggctatatc ttccccaatc ggctgccctt
      121 cgggtcaagg tggggtgcac ccacaggtgc aggtatgggc ttgattggtg tcgccgctgg
      181 cgcagtcgga gtccgtccag cctccaccgg cgaccgtctc gagatccagg tcggatagtt
      241 caccgacccg gtcgtccacc gacctgccgg gaagcacgag gtagatggtg tctgcggtct
      301 cctcgaccgc ctggatcgtg accccctccg gcagcggcat gccgagttcc tgctccaggg
      361 tggcccttgg gtcggcgagc agtcgccgtc tgaagtcctc atcctgaagg ctgcgttcga
      421 tgagcctgcg ctcgatctcc acgcggccgt tagattcggt cacgattcaa tcccccttcg
      481 ttctcggcaa caggagatga tccccactga gatcatggtg gtccgcctcg ccgtcgtcag
      541 gacccgtcgc gcagcggatc ggctacggaa gccctaggcg caccccgcgg tgctctcggt
      601 tgccatacac gtgttgggct cccagccgcc ggccaccgcc tcgagttcct catccgagag
      661 ttcgtcgttt tcggcgccgg ccgacgaggc gctagggaga acgaggtaga tggtgtctgc
      721 tgtctcctcc accacctcca ctcgcatctc ttcgggcagc ggtgctccca gctcccgctc
      781 catggtagcc ctcggatcct cgagtagcct cttacggaag gactcatcgt tgagggtccg
      841 ctgggcgatc ctacgctcta tctccatccg ccctgacgcc tcgctcatac cgaaacccct
      901 ctctcgatcc tccgccgata cggcctccgg gtgatcgcgc ccgctggctg caacccacgg
      961 gccgcaaggg acattatctc accctggccc ctcccgggct actgagtggc tcgcctccct
     1021 cggtagttgg cccgctaata tccgctcgcg gccgggtcct tccccaaccc gttctgctca
     1081 aggagatggg gaagatcagg cagggcggcc acgaggggga tcttgcgggt tccacggttc
     1141 ccgtgcggtt tcgccgtgtt cagggccgcc ttcggcaccc cggcgaacgc ggcaagggcg
     1201 tcgaggctcg tagagatgcc gtcggtaggg atcaccagcg accgttcggg tggtagtgcg
     1261 gcctcgatgc gggccgtctg gcgcgcgtgg aaccgtagca ggtctaccgc gagcgggcgg
     1321 agcgtggcgc gcagatgctc ggcggaagca aactgtccgg gatcgaggct gggcgccatg
     1381 agcctgccga gcgcgaggtg ccagtcgggc catgggcggc catcgaggaa ggcgccatac
     1441 tgcaacatca tgttcagaaa ggagtcggcc caggagacgg catcgcgttg ggtgtacacg
     1501 aagcgggcct cggggtgaag ctcgaccagt atgtccagat agtagtggtt gaagctggcg
     1561 gagtccagct ccagggtgcc ggcttcggcc cgtcgtgcca gccagcgacg catgccgtcg
     1621 cggcttagcc tgccgttcct ccaggcgacc aggtgcacca cggtctcggc gaagagaaac
     1681 tcgtagcccc agcggaaggg ctcgaagagt agcgccaggg aggtcgttcc ggtcttgggc
     1741 aggccaaccc cgatgaggtg gaaccgccgg cctggcaggg aggacgggac ctccttctca
     1801 ccccaccagt ggccacggtt gacgccctgg cgctgcgacc gcagcgagac gatgtcggct
     1861 tcaaccatcg gacacatcgg agtacccctt gcccgccctt gatgacagac gtcgatgggt
     1921 gtggaccagg ccgtcttcgg gatgctgcct cccgagctcc cgctccacga ccatcgtggg
     1981 gttacaggac acccccgggc caacctctct gtaccgtagg cttccaccgt tctgtccgcg
     2041 cttcatatcg gcacgtctat cggttccttc gaccaggatt ctagctccct ccgcttgcgt
     2101 ctgtacggcc ccaggtgcga gatatcgtta tcccacatct ccccggcccg ggcaaaccgg
     2161 cgggctgcag cccgtcaaac gtcctgcgcg gttctgaagc gctggcgacg tgatcccggg
     2221 acggaccggg gtcgggttgg ataaccctgc attcgatgct cttcatcata tacaatgtgt
     2281 aacgctgacg tgggagtcct acgctgagtt tgccggcgaa tggttgacgg ggtgtcgtgc
     2341 aaaagtccct gtttcgcgaa gatgctatgg acaagatgct gtctccggac gagctcgacg
     2401 agctcatgcg tgtcacggat cccagaggct ggctggccct catcgcgctg ctcgccctcg
     2461 tgatagccgc ggtcgggtgg agcatctttg ccagcgtccc ggtcgaggta ggcggtgacc
     2521 agggcgtcct gatccacagc ggtgccaggc atggggttgc atcccaggtg tccgggctcg
     2581 tgacgcaggt agaggtgaag cccaaagacc aggtgaaaga ggggcaggtc atcgccaggg
     2641 tccagaccga tagcggcgag aaggccgatg tggtcaccct ctacagcggc agggcggagg
     2701 aggtcctggt ccagaaaggc atggtgttga atcggggcca gcaggtcgcc gtggtggagc
     2761 agggcagcaa accactcgaa gcggttatct tcgcgcctac cgagcaggga aagcaactcg
     2821 aaaaaggcat gcgggtgcag atctcgccta ccaccgccaa ggtcgaggag tacgggttta
     2881 tggaggggag ggtcacctcc gtttcggagt ttccggtaac cagaggcgag atgttcgcgg
     2941 tgcttgcgaa ccagagcctg gtcgacacgc ttcgcacgag tgaggatcag ctccaggtcg
     3001 acgtcaagct gcagagcgac ccgtcgaccc cgagcggttt cagttggtca tcctcaaatg
     3061 gtccgccctt ctccatcagc cacggaacgc cggtcacggc aaccttcgtg ctgggcaagc
     3121 agcatccaat aaacctcgtc ttcccctcct cgaacgaata gcaacttgtc ggggtcgaca
     3181 cgacaacggc agcccgccac cccgccggga gaccgagacg caaggcgggg cgctggccgc
     3241 gcgaggacgc ctaccgtgct ccagatggag gcggtcgagt gtggcgcggc cgcgctcggc
     3301 atcatactcg ggtactacgg cagggtggtt ccgctggagg agttgcgcgt cgcctgcggc
     3361 gtctcgcgtg acgggtccaa ggcaagcaac gtggtcaagg ccgcccgggg gtacgggctc
     3421 atcgccaaag gttacaggaa ggagcccgat gagctgagga cccttgcccc accgtttatc
     3481 gccttctgga acttcagcca tttcctcgtg atcgaaggct tcggaagacg cggcgtttac
     3541 gtgagcgacc cagccaacgg acgccgcatt gccacctacg acgagttcga tcgggcctac
     3601 accggcgtgg tgttgacgtt cgagcctggg ccagagttcc gcaagggcgg ggccagacca
     3661 agcctcctcg ccgccatgag gacgcggctc aggggttctg aatgggccct cgccttcgtc
     3721 gtgctggcga gcctggcgct ggtcgtaccg ggcatcgtca tccccatctt ctcccagata
     3781 ttcgtcgacc agtatctcgt gcaaggctcg cgggacatca tactcccgct ccttggcggc
     3841 atggcgctta cggctttgtt gcgtgccggc cttacgtggc tccagcaaca ctacctgttg
     3901 ggccttcaga ccaagctctc cataagcatg tcgagcaagt tgttctggca cgcgctgcgc
     3961 ctgcccatgg agttctacgg ccagcgctac gcgggtgagg tcggctcgag ggtaacgata
     4021 aacgacagga tcgcccagtt gctctcgggg caggtggcga cgaccgccct caacctcatc
     4081 accgtcctct tctacgtagt cgtcatgctc cagtacgacg tcccgctcac gctgatcggg
     4141 atcgtcttcg ccgcgttcaa catcgcggtc gtgcggttcg tctcccgaaa gaggatcgac
     4201 gccaaccaga ggctactgct cgagcggggc aagctcacgg gcgtctccat gggcggcctg
     4261 cagtcgatcg agacgctgaa ggcgacgggg agcgagtccg acttctttgt gcactgggct
     4321 ggcctggagg ccaagttgtt ggaggcgcag caggaattgg gtgccagaac ccagttagtc
     4381 tccgccgtcc cgccgatgct gacggccctg accaccgccg ccgtcctggg cgttggggca
     4441 ctgagggtga tggatggcga cctgacgatc ggcatgctcg tcgcgtttca gtccctgata
     4501 acgagcttca tgacgccctt cggccagatg gtcgccctcg ggaccaccct gcaggagacc
     4561 aggggcgacc tgaaccgcgt tgacgacgtg ttgcgctacg agccagaccc gcaggtgcca
     4621 gcgaagatag gggcgcctga ggagatctcc ccggatctgc catccgagtt gtccggatac
     4681 ctcgagctga aaaaccttac cttcggctac agccggctcg aggcgccgct gatcgagggc
     4741 ttcagcctca ccctccagcc tggaagccgc gtggcccttg tgggcgccag cggcagcggc
     4801 aagtcgaccg tggcgaagct cgtgtccggc ctctacccgc cctgggaggg cgagatcctc
     4861 ttcgacggcc agcaacggac aaccgttcca cacggcctcc taacctcctc cctgacggtg
     4921 gtggatcagg acatcagcct gttcgagggc acggtaagag agaacctcac gctctgggac
     4981 gactcaatcg cccaggggag cctcgttcgg gccgccaggg acgcctgtat ccacgacgac
     5041 atcgcggcgc ggcagggcgg ctacgagagc aaggtcgacg aggacggggg gaacttcagc
     5101 ggtggccagc gccagcgact ggagatagcc cgggcgctcg tcagcgaccc gacgatcgtg
     5161 atcctggacg aagcgaccag cgcgcttgac cccacgaccg agggccagat agacgacaac
     5221 ctgcgcaggc ggggctgtac ctgcctgatc atcgcgcacc ggctcagcac gatccgtgac
     5281 tgcgacgaga tcatcgtcct cgacaggggc aaggtggtgc agcggggaac gcacgagcag
     5341 atgcgcgacg tggatggtcc ctacgcacgc ctgacagggg ccctggagaa agagaccagg
     5401 cgagaagggc ccacctgatg ccccccgagg agatgatgcc cgaggggcag cagtggttgc
     5461 tccggctcgg cgatcagaaa ggccgaagga tcgaggccgg cggaaacgcc ccgatctctc
     5521 tcgacgatcc cgactcggtg ttcgtggtta gctccggcaa ggtagacgtg ttctcgacgc
     5581 aggcggacgt gggtcagatc atgggtccgc gccgccacct gtgccgggtc accgccgggc
     5641 aggtcctctt cgggattgac ctgggccacg ccccagaaga gttcgggata ctggcggtcg
     5701 gcgtggccga caccgttctg atcggactga ggcgctccga gctcgcggaa tcgacgaagg
     5761 gcaccgtcca cgccggcctg gtgggtgctc tgctggaagg ctggttggag aaactctcct
     5821 ccgcggtggc agtacagcac cacactccca gagagtccag aactctggca cccggacaga
     5881 agatctccat cgacgctgac gagacggtcc gggcggagcg gaacacagtc tgggtcaggc
     5941 acctggatgg gagctcgcgc tttctcggcg acgctgatct acccccggtc ggcggggacg
     6001 gcctgttccc gctgtcggat ccggcgtggc tggcgtcggg cggcggtacg gccctggagg
     6061 gcgtgggcac ggtggactac gtggccggtg acgcatcgtg gtccggcctt gactctttcc
     6121 acgccgtggt cgtgtcggcg ctcgacctag agacgcggct ctccttctcc gaacggcggg
     6181 aactagagga cgagagggag tcggccgacg tggaactcgc caggagcgcc gtgaaccagc
     6241 tgggctccgt tctcgaaaag gacgccttca cggaagacga tgaccaggag accccgacgg
     6301 gcgatcccct gctggccgcg tgccaggtca tcggtaggcg gctgggtgtc gaacttcgcc
     6361 gccccgcgtg gtccgacggc acggcgcgcg acccgctcga ggagatcgcg cgtgcctccc
     6421 gggtccgcat tcggcgtgtc gccctcaagg gggattggtg gcgcagggat agcggccccc
     6481 tgctctgttt ccgggaggac gaaagcccgg tggcgttgct ccagaggggc acctccgcat
     6541 acaccatagt cgaccccgcc agaggaacca gtgacagggt aaccggggag acggctgcgg
     6601 agctggccga cttcgcctac actttctacc ctcccttccc ggaccggccg ctcggggcgt
     6661 gggacctgct caagtacagc ctgcggaacc tgcgggggga cctgctgcgg gtctccctca
     6721 tgggcgtggc cggggggctt ctcggggcgc tcgttccaat cgtcaccaag cagatagtcg
     6781 acatcgccat cccacaggcc gaaggtggtt tcgcggtcgc cctcgccctc ggtctcgcgg
     6841 tcgcggccgt ctcagccatg ctcttcgaga tgacccgcca gtttgccgtg ctgcgcatag
     6901 agacaaagct cgactccggc gtgcaggcag ctgtctggga ccggctactg agccttcccg
     6961 cccccttctt ccgcgagtac acagcgggag acctggcggt gcgggccatg ggcatcaaca
     7021 ccattcgcca gatggttaca ggcgccacca tatcttccct gttgtcaggc gtgttctcca
     7081 tattcagctt cgccgtgatc ttctactaca gcgcggagtt gggcctgata gcagcgctga
     7141 tggcggctgt catcgtggtc gtcaccgccg ccggcggctt cgtgcagttg cgttaccagc
     7201 gccgggagac cgagatgctg ggcaaggtgt ctagcctcgt tttgcagatg cttacgggaa
     7261 tcgccaagct gcgggtggcg ggagcggaga tgcgcgcgtt ctccgtctgg tccggacagt
     7321 actccatcca gaaggccaac gccgtcaggg cccaggcggc cgcgaatgcc ctcaccgtct
     7381 tcaactccat ctccggtgtt ctgacctaca tggtgatctt cgccgtcgtg gcctacgtgc
     7441 ttgatggtgg cgtgagcacc ggcgagttcg tcgcgttcaa caccgccttc gcccagttcc
     7501 tgttcgcctc cgctggtatc accacggcgt tcacgacaat ccttcaggca gcccccttct
     7561 acgagagggc caagccgatc ctgcgcaccc tgcccgaggt ggatacggag aagccggacc
     7621 caggtgtgct gaggggccgg gtagagatca accagctctc tttccgctac gatcccgatg
     7681 gccctctcgt gctcgacaac gtctccctac gcgccgaccc gggggagttc gtcgcgatcg
     7741 tcggcccttc cggcgccggc aaatcttcgc tcttccggca gctgcttggc ttcgaggacc
     7801 ccgagtccgg gtccgtgtac tacgacgacc acgacctgac cgggctcaac gtccaggcgg
     7861 ttcgccgtca gctcggggtg gtgctccaga acgggaacat gatgtcaggc accatcttcc
     7921 agaacatcgc gggcgtctcg gacctcacca tggaccaggc ctgggaagcg gctcgcatgg
     7981 ccgggctcga caaggacatc gagatgatgc ccatggggat gttcaccttc ctcggccagg
     8041 gcggctcttc cgtctcgggc ggccagcgcc agaggctgat gatagcccgg gcgatcgcaa
     8101 cgaaaccgcg ggtactgctc ttcgacgagg cgaccagcgc cctagacaac aggacgcagg
     8161 cgatcgtgag ctccagcctg gagagcctga aggcgacccg catcgtgatc gcccaccggc
     8221 tcagcaccat catgaacgcg gacaggatct acgtgctcga ggcaggccac gtggcgcaga
     8281 gcgggacgta cgacgagttg atggagcaag aggggccgtt ccgggacctg gccatgcgac
     8341 agatcgcgta gagaggggat aggggatcgc cacggaaccg ccgatgatgg actctttcga
     8401 gggttacctc gggccggtcg ccgcaggggt cgcgccggag ctgatctctc cttccgcctt
     8461 ctcggatata aggtccgtag ccggggtgct gcctggaacc ctggcctaca acacgttcgg
     8521 cttcgagtgc cgcctggggg aggagcttcc gagggcggat tttctggtgc tggccaacgc
     8581 gtactgcgga cgggacagcc tggccggctt gcaccccacg agcccgctgt caccacgcct
     8641 gagcgctgac ccgatctgga ggcgcgtggc ggccttcgct tcccgctggg ctgacgcctc
     8701 gtccctgcta tacaacaccg ccgacaatgt ctggttggag ttcgatgtcg agggcccggc
     8761 tcccgatgtt cccgtaccaa gccttttttt cggcccacga acggacggaa gggacggagg
     8821 ggcggccctc aggccggacg tcgaaggata cagggcgacc atcgtggagg cgctgcgccc
     8881 tctctccagg ggggagccac cggccggtat gcttgaaacc atgtcgggtt gcctgcgcgc
     8941 ccttgccccc gacgatctcg ttttccaggt tggcctgatg atgtcaaggg gcgccgagac
     9001 cgttcgcctg tgtatacggc ccggttctgt ggagcgtatc accgagtacc tcgccgaggt
     9061 gggctggacc ggcagcgggg aggacctccg ggctattctc gagccagtag agcgcctggt
     9121 cgattacgtc tgcctggaca tagatgtggg cgacaccgtt cacccgaaga tcggcctgga
     9181 gtgttacatc cgggggcccg ggcaggccag gatccgggcc aggtgggagg ctttctctgg
     9241 cttccttctc gagagtggcc tttgcacccc cgacaagcgc gaggcgcttc tggcttaccc
     9301 cgggtacatc gacgagaacg cggagggggt gtcctggcct gtgcccctgc ggcgggcctc
     9361 gctcctgctc ggtggacgct cgctgagcat gttcgtgcgg tctatacacc acgtcaagat
     9421 cgtgtgccag cagggcaggc cgaccgaggc gaaggcttac ctggccgcca accaccactg
     9481 gcacacgcgc gcagaggcgg agaacaccgc cccacgcgaa cctatgcgca cgtgaggatc
     9541 aggtagcccc accccgcgta tcggctttgt tcgtagaaga gacccatgct acgatgcccg
     9601 acgcgagtac aaactggcca gggaggaagg gaaagtgaac gaggcgaccg gccgcaccga
     9661 gatggagcgg agactgatag agaggagcct ggaggacgaa accttcaggc agcagctgat
     9721 cgacgacccg agaaacgccg tagagcggga gctcgggaca cgcctgccgg aagagctgcg
     9781 ggtggtggcc gtggaggaga cagccgacac catctacctc gttctgcccc ccgcctcgca
     9841 agccggccag ccagacgaag gtctctccga cagcgatctc gatgccgtcg ccggcggtgg
     9901 cacctgggta gataacacct gtggtgggca gacggcgtgc ggctgcgccc caacggccgt
     9961 cggccgcggt gtcggctgct agggcagctt gcggagggat cgaagtccct gaagggctgc
    10021 cagccatgag cttgttcgct gaccgctgaa gactgatagc ttccacttca gaggacgaac
    10081 acttctgcag ttcactgcag aaggttgacc gattccgaga tccggccaac tgctcccggc
    10141 cccgccaggt cttcgagcct gaagccggtc agggcgagca acatggcggc acgctcctca
    10201 ggtcctcccg gtaaagagcg tccggcgcgc gagctctcga ggttctcgga ggcatctttt
    10261 acgagcgctt gcaggacacg gaaagcgccg tccgagaggg gtggttgtcc cctgtagttc
    10321 tgcaatcggc cgccctcccg gtaggctggg aaagactcga gacccatgaa gcgcagaagc
    10381 cagtcggacc gataccgtcc ggcggcggcc gtgatcccca tgaagtccgc gatcagctcg
    10441 tcgaggatgt tgttgcgcat cgaaccgaag acccgcaggg tgaagtagtg ggtgcactcg
    10501 tgctcgagcc ggatgctcgt ggagagccgc cgccactcat cctccgtaaa ccccatctcg
    10561 ggggccgcca cgccgctgta gggaccgtcg ctcaggatga ggaaacggtc ctggtatagc
    10621 gccttgcgag gcacgaggcg ccggaactct tccttccagg cggcctcgga ggtggagccg
    10681 ttctcaccct cccaccgccg acgatactgc cggattcgtt cccaatcgtt gaacccggag
    10741 accatgcaag cccccatcga accgggtacg ggtcgcggct cgttcctcat cgtaagggcg
    10801 cgtaccaggg acacgaagtc ctcccggccg ccggggatca ggaccggtat cggcccggcc
    10861 gggctctggt ggacgcggag cgtcaactct tgtggacgcg tgagcacgag gccggatgcc
    10921 tccgccatcc cttccgtgga tgcccccctg agcgtggcag cccgataggc ctccgtctgg
    10981 ctgatgccct cctctatggg aaaccgcagt tgcgccagcc ggcgcccgag cgtgacgaag
    11041 gcgttggtcc cggcagcctc cctggcgtat ccgacccagg ccgccacgtg gggctcggcc
    11101 gggagcggaa gcaccggcgg ccacccttcg ccggaccgat cgaaggtgtt ggtgtcgtat
    11161 gcttgaagtt cctcgacctc ggagacggtg gcgccgtaag acgcgagcag ttcgtctcgt
    11221 tgctctgggg tcatcatcgc tatctcctcc cgaccgagcc catttcgaga cggcgtcttc
    11281 gcaccccgtg aaaaaccgag ggagggtcag tgtcacacga gcccctttct cgggcaaggc
    11341 aggcaccgcg tcccgacgaa atatagtatg cgggcgatgg acgaccggta cctcatggac
    11401 gatgggcggg agatctcctt ccaccgtgac agcggcgggg accatctcgt cgttccagtc
    11461 caccgcgccg agaaggagcg tctgtcagaa tgcctgcggc ggctcgaatc cggtggcgga
    11521 cgactcgatt actctctgca ggaccacggc tcatcctcca cctccctgcg cgtgagcgcg
    11581 gggggagccg acctcgataa cctctggacg cggctcttca cccgtcttca ccgctacccc
    11641 tactatgagg aggtccaaac cgagtccttt agaaacgccg ccgagccggt gctctcctgc
    11701 gtcgtattgc ttgcgggcaa cgaccgcttc gtacagaggc acctcgtacc gagcatcctg
    11761 gccaacagcg agggttaccc gatcgagatc gtcatcgtct acaacggtca gtgtctcgat
    11821 ctcggcccct ttcggaacct ccgcgtcgtg cgctcagatt tcggatgggt ctcgagggcg
    11881 tacaacgccg gggcaagggc ggcacgtggt cgctacgtcg ccctctttca cgatgactgc
    11941 atggtcgttt ctccgcgctg gatcgggacc agcatacgga tgctcgaggc gggctacgtc
    12001 gcggtcaccc cggaggttcg acgaaagacg accacgtggg gtaccgagct gcttatcgcg
    12061 aagaacgtgc ccctgataat gcgtcgtcgg gacttcgtgg cctcgggcgg ctacgacgag
    12121 ttctattacg ccggttacga ggaccaggat tttacgtaca ccctgctctc cggtggaggg
    12181 aagatcgggc acctcgacct tcccttcctc cacttcaatg gcatgagcac ggtcgtcctc
    12241 gctggcggtg cggccgggct gttcagggtc ctgttcgggt acaacgcgct ggggcaggac
    12301 ctgatctacg agttgcgcga agagtctttg cgaagtttga gatcccatcc gaagatcggc
    12361 ctttccgagg cgagggatac gctctacttc gtgaggaagc acaaagcgta cttcgcggag
    12421 cggcagaacg aggccgtcct ggagatgggc caggccatgg acgaatatct tgccgcccac
    12481 ctcgccgagt acctgttcga ccccgtcctc caggaccggc agaagttcac cgaattctac
    12541 aggtcagtgc tcggccttcc cgccgaatag gctcgacgac cgcacggcgc tagccggccg
    12601 ggttacgccc gtcgagacga ccaggaactg atagctgaca gctctccttg gagacagtcc
    12661 tctagaggac taccctaatg gcagaagttg gagcagatgc actgggattc gtcgctaccc
    12721 cagccgccgc ccccggccac ggcctccagc tcgtcgtccg agagagtacc acccgtctgg
    12781 tcgggcgcgg aagcgggagg cagaacgagg tagatggtgt cggctgtctc ttccacggca
    12841 cggatctcca tgctctcggg aagcggtgtc ccaagctcct gttccacgac cgccctcggg
    12901 tcggcgagca ggctctgcct gaaggactcg tcctcaacgc tcctctggac cagcctctgc
    12961 tcgatctccg cgcgaccacc ggctgcttcg ctcattagtc ccctccctgt agtgggtccg
    13021 aacctccaac tgacaccccg gacaccccac gaggtgcgcg caccagtctc tcacgttgcc
    13081 cattgccggg caagcgacgc gcctggcgga accggcctcg agaccatagc cgggggcggc
    13141 tccctttgct attctgacga ccttgtggag cgcggacggg ggtacgcacg tgccggggtg
    13201 ggtgcgttac tcgacccgga aacggtcgca cgcgcgtcat cgaggagaac cacgacagag
    13261 gtgctagaca ctgcaagcgt ccgagataat acgtaacctg cccgaatcgc tggactggat
    13321 ggtgctgttc gacctgcata ccctgcgcgg gatggccgac gacacctccg tcaggggcat
    13381 gttctacctg ccgcagagca cggacctcga cctccacagc cacgtcatcc tgaccagcca
    13441 ggggacgttg gcagcgagtc ggaatggatc caggctggtc gatcccctga gcgagacggt
    13501 ctttcccgcg gttgggaagg gtacgtctct gtacgagcgg ttctctgacc gcttcgacct
    13561 gttcccctcg gacgacgttg attgcctggc gataggcgag atggcgcctt tccccccggt
    13621 cttgctgcac ctgcgggccg agaacggctt cggcgaggcg aaggccgtct ttgcgcggga
    13681 accctccacc ctgcattacg agctcctcaa ggccataggg gtggagtacc tgggtgggaa
    13741 ccccgaaggg gcccactacg tggcccgttt tcgaaaccat ctgccgaccc acatccactc
    13801 cggggtgctg gcgcactttg cccgcacgag ccactgcaac atgttcttct tccggcacgg
    13861 ttcgatcgat agaccacttg aacggggtct gatctcggcc tccacaaaca gggtgtcgtg
    13921 ggcggcaggg cgggctcgcg aggctgtcgt ccggatggcg aacgaggccg aaggcgaaga
    13981 tgcggtatca ctcgggttcc tgctccgggc gctgaaccac cacacctccg cggcctccat
    14041 cttcgacaac atcgatacgc gcacgaggct cgtatctctt ctacgtgacg gagagcacga
    14101 ctcgctctgg ccttacgagg tcggcggccc tgcctcctcg accgcgtcgg cgatcgtact
    14161 ccagggcctc tatgaccctg caggggtgga agcgctcgag gcgttcgccg atggccgagg
    14221 agggtactat tcccggctgc cagcagacca gcgggagacg gccagcgatc cggccaacag
    14281 cggtgatggg catcatcacc cggacttcgc gaccgcttgc atgatccgtg ccctgagaaa
    14341 cggtgccggg ctggatacgc gtaccggcgt cgagtatctg gcgacgcgtt tcgagggcag
    14401 gagcggcctg tgcttcgcca acccgtacct ggtggattgg gcgctcgcct ccgcgctgga
    14461 gaacgatgac tcggcgcagg agttgcggaa tatgctgacg agcgagatcc tggccagcat
    14521 gaacgacgac tactcgttcg gtcgcttcga cgtgcccctc tccagcgcgt tcgcgatctg
    14581 tagcctggca tccctcgggt tcaggagccg aacccttcgt ctcgcccagc ttcgcctgct
    14641 cgacttcatg cagccggacg gcaccttccc cgcggggacg cccctcttct ccgtacgagc
    14701 ggataccaga gagccaggct gcgggcccag ggacgtcttt ctccgcgccg accggcacgg
    14761 aaccatctcg acggcggctg gcacgctcgc cctgctcgcg gcatgctcgc cggaggaggg
    14821 ggatccctac gccggcgggg agtggggggg aggggcccac ccgcgctatg gctgccggga
    14881 tcacgaggat tacatacgga ggttcgtgct gccacccgac ggcgaaggat tgtcgtgagg
    14941 ccggccacgc atcggggcca gtactggagc ggctcccacg gacgtaaagg acaacactct
    15001 cgggctgcgg gtgaaagctt tcggcctgac ggatcaggaa cgaggcgcta gtgaggcaaa
    15061 tcgacgtgct ttcggagcag gacctggtgg agatcgtcga gcgcgccagc tcggtggacg
    15121 aacggctggg cgacgatttc gtaccggccg ccgacggcgg aagcgctgaa gcggtcgacc
    15181 gtcgcctcga agcatggtca caggccctgg gtagggggga cgaggaccgg ttccggcggc
    15241 ggctggcctg ggacggcctg gaaccggaat ctgttcggcc cgtcctcggc ccggtgcggc
    15301 tgcgtgaggg cgtcaggcta ccggagtggt ccgggctgat cgcggaggtc cttcgcctgg
    15361 ctccgccgga gcggcaagat ggtgcggaag aggcatggcc ggaggagttg agcttcctgg
    15421 atgccgagga tgtcctgcca ttcgaagaga tcctcgcgcc gttcgtgctc gtggcccgcg
    15481 agaggctcgc cgggcgcgcg gcagcctacg gaggattgct ttcggcgggg gcgcgcaccg
    15541 acctcgaacg caccctgttg cgaagcctgt cgttcgcctc gggcgagcta ttcctggccg
    15601 agttcgaggc catgcgcgcc caggaacagt cgtcctggga tcgattgttc gcgctggcgc
    15661 aggatcccga gggacgttcc ctctaccggc ggttcgtccg aaggtcaggc gaggggggga
    15721 tggccagcct gttccgtcgg tacccggtgc tggcacgaca ccttggcacc atctcgcagc
    15781 tctgggtcga ggcgaacgaa gagttcctcg ggcgcctgga agaggatcta cccgagctag
    15841 agcgcctctt cggcgacgag aggtttggag aggtcgtcgg gctgggatct tccctgtcgg
    15901 acccccactg cggcaggcgc agcgtcctgg cgctgacgtt cgcctcaggc cggaaggtag
    15961 tgtacaagcc gaaggacatg ggcaccgagg cggcctacca ccggctgctg gcgtggctca
    16021 acgagcgggg cgcgccgctg ccgttcaagg tactcaaggt actcgaccgc tccacgcatg
    16081 gctgggtgga gttcgtggag tatctgccgt gccaggacga ggacgaggcc aggcgctact
    16141 acgagcgggc cggcatgctg ctctgcctgt tctacgcgct cgaagggacg gactgccact
    16201 acgagaatat catcgcgagc ggcgagtacc ccgtgctcat cgatacggag acgttgatgc
    16261 accaccgcgc ccgtgcggtg atcccggacg atgagaacgc gcagacggag gccttcgaac
    16321 ggctgggcca ttccgtattg cgcaccgggt tgctgccccg ctgggagttg agcgtggacg
    16381 aaggtaaggc ttaccacgtc agcgggttgg gcgaggcgga agagcaggag tggccgatcc
    16441 ggatgccgac ctgggtaagg aagaacacgg accggatggc cctggagtac gatttcgtca
    16501 agctccagat gagggagaac gtacccacgc tagagggtgt ccccctgagc ttgcaggagt
    16561 atgccacgcc tcttgtggac ggcttcaggc gcatgtaccg gtttctgctc gaacaccgcg
    16621 aggcgcttct cacagccccc ccgctccagg agttggcccg tcagcaggtc cggttcgtgt
    16681 tccgcgccac gaaggtctac gggctcatcc tccgcaacct gctacaccgg cagtacctgc
    16741 gcgacggggc ggatcggagc atacagctgg agcagctagg tcgggcccag gtgccgcccc
    16801 gcgatgcctt agacgagccg gacgagaggc cgatcttctg gcccgtgttc gcggccgagc
    16861 gcctggcgat ggagacgggc gatgtccctt tctttaccgc cagggccagc agcgacgccc
    16921 tgatcctcgc cccgggccag gaggtcgaag ggtgcctcca ggagccaagc ttcgagctgg
    16981 tctcgcgcgt gctcgaaggg ctgggtgata gcgatctcga acaacaggtg gcctttatcg
    17041 agggctcgct ctacgcccac ctggcccgcg agacaacggc ctcgcacccc gttgtcgagg
    17101 agccgcagga ggactttccg gccggaccgc cggagggtga ggacttcatc gcccccgcgc
    17161 tcgagctggc ggaggcgatc aagtctcgcg ccatccgcac ggaagatggc agcgcggcgt
    17221 ggatcgcccc ccagtacctg gttcaggccg agcgttacca gcttcaagtc atggatttca
    17281 gcctctacgg cgggaccgcc ggcgtcgcat tgtttctggc ggccgccgag cagttcgccc
    17341 ccgattccgg ctacggcgag ctggcgcagg ccgccctcag gccactccga agcgcgcgtg
    17401 atcgtcgccc tgaccgcttc gccgagagga tgggcatcgg tggtgcctcg ggcatcggct
    17461 cggtggccta ctccctgctc cacgtggggc ttctgctcga taatccggcg ttgctcgaag
    17521 aggcgcgccg ggtggcggac ctcatcaccg atgatctcat cgcctccgac aaggttctgg
    17581 acgtgatagg tggtgcggct ggcgctatct tgagccttac cgccctttat ggggtgatgg
    17641 gggaccagca gatactggag agggccatcg cctgcggcga gcaccttctg gagatgcgga
    17701 cggagacggg ggccggtccc cgcgcctggg cgacgagcga acagctgagg accacgggtt
    17761 tctcgcacgg caccgctggt attgcgtacg ccctgctgcg gctctatgag cacacgcggg
    17821 acgagcgact cctcgaggcg gcagacgagg ccatagccta cgaggacacc atgtactcgc
    17881 cggagaacca caactgggcc gaccacgtga agtccggcga gtcgaactac ctgtggcaat
    17941 ggtgccgcgg tgcgccgggc atcgggctgg cgcgggttgg cggccttgac gtcctggata
    18001 ccgggcgggt ccgtgaggac atcgagctcg ccatgcaatc gaccctccag tacggggtgc
    18061 ggggcgtgga tcatccatgc tgcggcagca tggggctggc ggaggtcctc ttgtcggcgg
    18121 gacaacggct cgcccgcccc gaactggagg catcggcgag gacgaacgcc tggcgggtac
    18181 tcgacaaggc gaatagaacg ggcgggttcg tccttcatcc cttgttgcca aggcaggtgg
    18241 acaaccccgg cttcttccag ggcacggccg gtatcggcta cgagctgctg cgcatggccc
    18301 gccccgattt gctcccttct gtgctcctgt gggagtaatc ggcgcgaagg agcgttgccg
    18361 aatctggttc ctgctttcta gcaacctccc tgggtgaacg gctcgcaacc cgcctgcatg
    18421 gtgttgtagt cccagccccc ggcgaccgcc tctagctcca ggacggagag ctagccgtcg
    18481 gactgcccgg tcccggctct ggggggcagc acaaggtaga tggtgtcggc ggtctcctcc
    18541 acggccgcca cccgcacccc ctcgggcagc cgcgccccca gttcctcttc aactgccccc
    18601 ctcgggtcct ccacaagcct ccgcctgaaa gactcgtcct ccaggctctt ctctatgagc
    18661 ctgcgctcca tctgctccct gttcgacgct tcgctcatgc gctccactcc cctttcgctc
    18721 cagccggctt gcctgttcgt ccctgcctgg cgcgggaatc agtatcccac cccacccatt
    18781 cccgggcaaa gagtcctgcc gtacgacggc gggcgacttg ctcgcccacc ctccattatc
    18841 gggagactac tcccaggaca cctaccgcga gccgagacgt tccaggacca tctatctgcc
    18901 ggacgtaccg ctcgggaggg ggtgtatctg gccgcgaacc aacgctggcc gaacggaggg
    18961 gaatgcgact acaattcgtc tctggtacgg gccacttcgt ggcctggcgg tccgatgaga
    19021 gggggagtgc aaggaattgt cgtcttccga ggaggcgttg gtcgaaacga gggtacgcgc
    19081 ccttgcggcg ctagcggagc tggccgggga ggccagccga acccccctgt ccctgatctg
    19141 ccagcccccg ccaccgacga gtcctttccc ttacggggac ctggtcccgc tcgggttcct
    19201 gctccgggcc gccaacgcct gcgggtcgaa cccgtccgtc cgaagctcac ttgaagacct
    19261 gctgcgcgac aaacgacagg ggctcctgtg ggcgtaccac agcgataccc tcgtaacctg
    19321 catcgactcg gcgctggtct tgcaagggtt ccgagaccag gccggcgtcg aggccctgga
    19381 gctcttcgcc gatggccggg gcggttacct tccccagctc tgcgcggaga gaccggagcg
    19441 cgaccacatg accatcaccg cgcacaaccg tcactggtgt cagcccgagt acggcaccac
    19501 ctgcctggct agcgccctta gggcggaggc gggcctggag aggaagacca gcgtcgaata
    19561 cctggcggag ggcttcgaga acaggggcgg gctgtacttc gccaacccct acatggccga
    19621 ctgggcgctg gcctgggcgc tgcagggcgc cacctcagcc accgcgctac gggagcgtct
    19681 ggccaccgac atcctcgcga gcgccaacga ggacggttcg ttcggccgct acgacgtacc
    19741 cctgtccacg gcccttgcca tcctggccct gatctcgctc ggcctcgagg gtgagcccgt
    19801 gacccgtgcg cgcctgcgcc tcgccgacat gcagttgccc gacggcacct ggcccgcaag
    19861 catcccgttc tattccgccg tgaagatgcc acacgagcgt ctgcccggtg gcctgctcgc
    19921 caggctgatg ctcggcgaga ggcggggcca actcttctgg ctagaaggtg gggcctacac
    19981 cgcctctctt tacgtcgacg ggtaccgcac gattagcagc gccctcgcct cgctcgccct
    20041 gacgcaacca ggtcctctta ccgggcttga cgccctaccg aagcaaagcc gggagagttg
    20101 ccaccctcgt taccgttgtg ccgaccacgt acagtacata gcgggctacg ccttaccccc
    20161 atatctcgtt ccgcgatgag cctacctcga caggaacccc ctgaatagga tggcgggttg
    20221 ggggcccctg agcgttgaca accgggcacg gcaggcgtat accggaaaac gacaatagca
    20281 tagccataaa gcctaactcc cgggagagcc ggggggcgtg ggacccagga ggatccggag
    20341 agatccacgg ggtgaatcgc cgggatctcc tggcgtaggg ctagagtctt tcggcccgaa
    20401 cccgacagct aaccccgcag gcgctgaaga aggaggtaga tggcatgccc gcacagcaac
    20461 ccttctggga gcagctgatg ttcggccgcg aacacggccc ccgcgccgag cgcgaagaga
    20521 aagtcctgcg ctacatcgtc caccggctgg atgaagagga tgcccacctc agcgacgtgc
    20581 tgcaggagac ctacgtgcgg cgcaactgca cgcaggagat ggtggacgag atcgtcagga
    20641 accccgagct gattcactcc tgccgggaac acctggagcg cgtcttcgat tcaggagagc
    20701 tcgacccaag aaccagacca caggtaagcc actagcgcca acccccgatc tcgcacaccc
    20761 aaccccaggg ttcggcctcc cggcaacgca tgcaatcagc cagaggctaa tagctcaagg
    20821 ctaaccagtg ccggcatgag gtaaggttct ggagaaagct agcaggttgg acggcgagag
    20881 gggagaggcg ggaccatgat ccagcaggac cgtgctccgg agaagaactg ggcgggcaac
    20941 tacgagtacc gggcacggaa gcttcaccgg ccgtcgacga tggggcaggc gcaggagatc
    21001 atcgccaacg caccgcgctt gcgggtcctt ggctccaggc actcgttcaa cgacatagcc
    21061 gactcctcgg agctcatcac gctggaggct ctcccagaag acgtggtcgt cgaccacggg
    21121 gcgagcacgg tctccctcaa cgctgccatg acgtacggcg agctggagaa gacgctgcgc
    21181 gcagaagggg tggccctgca caacctcgcc tcgcttcccc acatctccgt cggtggggcc
    21241 gtggccaccg cgacgcacgg ctccggcccg gagaacggca acctcgcgac cgccgtcgcc
    21301 ggcatcgagc tggtgacctc cgcgggggag atcgttgagt tcgccagggg cgatgcggac
    21361 ttcgagggcc tcgtggtcgg cctcggggca ctgggggcgg tgtccagagt caccctcgac
    21421 gtagagcccg cttacgaggt gcggcagcgg gtctacgagc gtatgggctg ggaggcgttc
    21481 tacgagcgct tcgacgaggt aacctctggc ggctacagcg tcagcgtctt tacccgctgg
    21541 gcagaggtca tcgaccaggt ctgggtcaag agccgggtga cggacgagcc ggagcgggta
    21601 gaggacgagc tcttcggcgc ggtcgcggcg gtggaggacc ggcacccgat cccgggggtg
    21661 gacgcgggcc cctccacgcc acagatgggg gtgccagggc cctgggccga gcgcctgccc
    21721 cacttccgcc tgggctttac gccgagcagc ggcgaggaga tacagtccga gtacctggtg
    21781 ccgcgccggc acgcccccgg ggccatagag gccgtgcgtt cgctcgccgg tgagatccaa
    21841 ccccacttgc agatgtcgga gatccgcagg gtggccgcgg acaggttgtg gatgagcatg
    21901 aactacgggg aggataccgt cggcctccac tttacctgga agcgcaggcc ggacgaggtg
    21961 ggggattgcc tcgtccggat cgaggccgcg ctcgcgccgt tcgaggcgcg tccgcactgg
    22021 ggcaagatgt tcgccgccga ggcggaagcg atagccccgc tctacccgcg cctgccagac
    22081 ttcgtgcgcc tgatggaacg cctcgaccca cgcggggcat tccgcaaccc ctggcttgag
    22141 gcgcgcgtgc ttggcgagag cgcccccagg cacccgtaag aagcggagcg gccaagcata
    22201 tacactgcga ccaatgagcg agagcgtcga gcacattcct cccctggccc tgatggtcag
    22261 ggcccccgat gagcccggtg cccttcacgc cctgaccggg gtgctcctcg accacgacgc
    22321 gaacattgcc cacgtagata ttgccgagcg gagcgatggg ctctccacgg tctacttcga
    22381 gctcgaggat gtcgcggacg gcgcggcgga ggtgctggtc cgggatctgg catccctgcc
    22441 catcgtgcag gtggttgagc tggcaccctc gttcctcaag gtctacggca agcgcataat
    22501 cgtggttggc ggtggggcgc aggtcgggca ggtggtggtc ggggcggtcg cggaggc
//
LOCUS       DBNGVD010000574         3584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1247001, whole genome shotgun sequence.
ACCESSION   DBNGVD010000574 DBNGVD010000000
VERSION     DBNGVD010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1247001"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC16CM_15475"
     CDS             <1..435
                     /locus_tag="AC16CM_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3141281.1"
                     /translation="ATPARLKGMPSWLLGRTAMHAHRLVVERLAAAGARGYHYRLLAA
                     LEEFGPASQASLGRRTGIDRSYVVEAVNELADGGLVERARDPNDRRRNVVTITQAGVR
                     QLLRLDGVLEGIQDEVFAPLSTDEREELARLLTRILDRHDPV"
     gene            complement(606..1769)
                     /locus_tag="AC16CM_15480"
     CDS             complement(606..1769)
                     /locus_tag="AC16CM_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HIR3141282.1"
                     /translation="MAELQKRTLEEVKALSVDDAVEIMRQAGIVGAGGGGFPTYFKYK
                     RPLPHLLVNATESEPGYWGDKLLHKEYLEEFLLVFEAMKEIFDFERISFCVHEKDTDW
                     FAEYAEHADEGVYEISYVPNTYALGEEKTLVKHATETRVPRFVDNPDGTRRPGMPPDV
                     GKVVNNTETLLNVYRALFLGKPLTTKFFTVYGEGVRTQVLEVPIGASVTEVLRLSGVD
                     VDNADHITVLDGGPYLHDMAIAELGAGEDAYVRRMTNSLFLIPHGSQGKEFAGIETEC
                     PEEGIASLVGEVSGVNLPLGGGLLKPATPLVSEGDEVEYEQKLGEPVDEGFSVGVWAS
                     MGGEISSLENGIVAISGGAITQEEAEAEAEPSMAGGSQPRGEAEPFSEAGSSS"
     gene            complement(1773..2036)
                     /locus_tag="AC16CM_15485"
     CDS             complement(1773..2036)
                     /locus_tag="AC16CM_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR3141283.1"
                     /translation="MAYVITEACIGTKNTACVQACPVDCIYDAGDHFIINPAECIDCS
                     MCMPECPVEAIYPGDEVPEDMQYYVSRAAEFDYSDTAPGVEAN"
     gene            2591..2941
                     /locus_tag="AC16CM_15490"
     CDS             2591..2941
                     /locus_tag="AC16CM_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3141284.1"
                     /translation="MIYLYVGIFMLLVTGFVATTLILARLISPGRASSTKGQNYETGE
                     VTVTDPWRPFPVRYYVFALLFLVFDVEAAFLFPWAVIYKSLGLYGFVEMVIFVFILAV
                     GLVYAWKKGALKWV"
     gene            2932..>3584
                     /locus_tag="AC16CM_15495"
     CDS             2932..>3584
                     /locus_tag="AC16CM_15495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3141285.1"
                     /translation="MGVMQQFNEPNIVTTSTDKLFNWARKNSLWPLQFGLACCAIEMM
                     STGMAHNDLDRFGAGFFAASPRQADVMIVSGTVSTKMAERMTRLYEQMPEPKWVIAMG
                     ACAISGGPFLDGYSVLMGADRVIPVDVYVPGCPPRPEALIFGIMTLQKKIERDQQVGY
                     DRPRPALVDEDGTINEYLPEREYTGISEGKAPRLEGIRPLQRTYVFERAGMPPASQT"
BASE COUNT          606 a         1115 c         1154 g          709 t
ORIGIN      
        1 gcgaccccag cccggttgaa aggcatgcct agctggctgc tgggccggac ggcgatgcac
       61 gcccaccggc ttgtggtgga gaggttggct gcggccgggg ccaggggcta ccattaccgc
      121 ttgctcgcgg ccctggagga gttcggcccg gccagccagg cgtcgcttgg tcggcgcacc
      181 ggcatcgacc ggagctacgt cgtagaggcg gtgaacgagt tggctgacgg gggactggtc
      241 gagcgcgccc gggacccgaa cgaccgtcgg cgcaacgtcg tgacgatcac ccaggctggc
      301 gttcggcagc ttctgcggct cgatggggtg ctggagggca ttcaggacga ggtgttcgcc
      361 ccactttcga cggacgagcg cgaggagctg gcgcggctcc tgacccgcat cctggaccgc
      421 cacgatccgg tatagagcgt tttacgcaac agatgatggt cgtgtgaggc cgcgcgactg
      481 aacccttgcc cggtcgacct caggctgaag gtgctagacg ccgcgtacgg gccagagttt
      541 cggtttccga cacgggccgt gcggtgcgca aggagggctg gacgttcgag aaagaacgcc
      601 cccgtttagc tgctagaccc tgcttcgctg aagggttcgg cctctccgcg gggttgcgac
      661 cctccggcca tgctcggttc cgcttcggcc tcggcctctt cctgggtgat ggcaccacct
      721 gagatggcga cgatgccgtt ctcgagggag gagatctcac cacccatgct cgcccagacc
      781 cctaccgaga agccctcgtc cacgggctct ccaagcttct gctcgtactc gacttcgtct
      841 ccctctgaaa cgagcggcgt ggccggcttg agcagaccac cacctagggg caggttaacg
      901 cccgagacct ctccgaccag cgaggcgatg ccctcctcag ggcactccgt ctctatcccg
      961 gcgaactcct tgccctggct accgtggggg atcaagaaca gggagttggt catccgccgc
     1021 acgtaggcgt cttcgcccgc accgagttcc gcgatcgcca tgtcgtgaag gtagggccca
     1081 ccgtcgagca ccgtgatgtg gtccgcgtta tcgacgtcaa cgccggagag ccggagcacc
     1141 tcggtaaccg aggcgcctat gggaacctcg aggacctgcg tgcgcacccc ttcaccgtaa
     1201 acggtaaaga acttggtggt gagcggcttg ccgaggaaca gcgctctgta aacattgagc
     1261 agggtctccg tgttgtttac gaccttgccc acgtccggcg gcatccccgg gcgcctcgtc
     1321 ccgtccgggt tgtccacgaa ccggggtacc ctggtctccg tggcgtgctt gaccagcgtc
     1381 ttctcctcgc cgagggcgta cgtgttcggc acgtagctga tctcgtagac gccttcgtcc
     1441 gcgtgctcgg cgtactcggc gaaccagtcc gtgtccttct cgtggacgca gaaagagatc
     1501 cgctcgaaat cgaagatctc cttcatggcc tcgaagacca gcaggaactc ctcgagatac
     1561 tctttgtgaa ggagcttgtc cccccagtac ccgggttcgc tctccgtggc gttgaccagg
     1621 agatgcggca agggcctctt gtacttgaag taggtgggga aaccgcctcc cccggcgccc
     1681 acgatgccgg cctggcgcat gatctccacg gcgtcgtcta cgctgagagc cttgacctct
     1741 tcgagcgtcc gcttctgtag ctctgccatg agctagttgg cctcgacgcc gggcgcggtg
     1801 tcggagtagt cgaactctgc ggcccggcta acgtaatatt gcatgtcctc gggcacctca
     1861 tcgccgggat agatggcctc caccggacac tccggcatgc acatggaaca gtctatgcac
     1921 tcggccgggt tgatgatgaa atgatcaccc gcgtcgtaga tgcaatctac cggacaggcc
     1981 tgcacgcagg ccgtgttctt cgtaccgata caagcctcgg tgatgacgta ggccacgacc
     2041 ctcccttatc caggtggcag acgtgtccag tctagcacgc tacgccttcg gcttcgcagc
     2101 acgccgccct ccgggcggct cgtcagcgca ctacggcctt cggcctccgt ttgtaagcac
     2161 gctcaggctg ccgccttcgc ttgtcagcaa cgcttcgccc ggcttcgcct agagctcgct
     2221 ttcggctagt aaggctgcga cctgaccttg ccgcttctga gtaccgccgg gagggaatat
     2281 tgcggttacc gagcttgccg tggcggtccg agacgaccgg ctgaaagcga ggcgaagccg
     2341 aagcgttgcc gacgagtgag gccgaaggcc gaaacgtgct gacgagccgg cggaggcgct
     2401 agccgaagcc ggcatgctga ccagcggagg cacgtggggc cgaagcgcgc tgcgggcggc
     2461 ggcttgcagc cgcttgctac tgttagtagc ttcttgaaat ccggggcttg cgcgggtata
     2521 ttgcggttgg atatcgggta gaaaggctgt tttggttcta ggtctgttgc agcaagaagc
     2581 atcttacggg atgatctatc tgtacgtggg tatcttcatg ttgctggtca cgggtttcgt
     2641 ggcgacgacg ctgatactgg cgcggttgat ctcgccgggg cgtgcctcca gcaccaaggg
     2701 gcagaattac gagactggtg aggttaccgt taccgatccc tggcgacctt ttccggttcg
     2761 ctattacgtg tttgcgttgc tgtttctcgt gttcgacgtg gaggcagctt tcttgtttcc
     2821 gtgggccgtc atctacaaga gcctcgggct ctacggtttc gtagagatgg tcatcttcgt
     2881 gtttatcctg gcggttggcc tggtttacgc ctggaagaag ggggccctta aatgggtgtg
     2941 atgcagcagt tcaacgagcc caacatcgta accaccagca cggacaagct cttcaactgg
     3001 gcccgcaaga actcgttgtg gccgcttcag ttcgggctcg cctgttgcgc catagagatg
     3061 atgtcgacgg gcatggccca caacgacctc gaccgcttcg gggccgggtt cttcgcggcc
     3121 tccccgcggc aggccgacgt catgatcgtc tctggcaccg tctcgaccaa gatggcagag
     3181 cggatgaccc ggctttacga gcagatgccc gagcccaagt gggttatagc gatgggggct
     3241 tgcgccatct ccggcggccc gttcctggac ggctactcgg tgctcatggg ggcggacagg
     3301 gtaataccgg tcgacgtgta cgttccggga tgcccgccgc ggccagaggc ccttatattc
     3361 ggcatcatga cgttgcagaa gaagatagag cgcgaccagc aggtcggcta cgacaggccg
     3421 aggccggcgc tcgtggacga ggacggcacc atcaacgagt acctgcccga gagggagtac
     3481 accgggatca gcgagggcaa ggccccccgc ctcgagggta tacgtccact ccagcgcacc
     3541 tacgtgttcg agcgggccgg tatgcccccg gcctcccaga ccga
//
LOCUS       DBNGVD010000575         4336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2707220, whole genome shotgun sequence.
ACCESSION   DBNGVD010000575 DBNGVD010000000
VERSION     DBNGVD010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2707220"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="AC16CM_15500"
     CDS             <1..713
                     /locus_tag="AC16CM_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3141286.1"
                     /translation="WSEEFIRFALEKSLERLDTDYVDFLQLHNTKMDAVEDDDLFALM
                     EKFKEEGKIRAYGVALGPKIGWLDEGVRAMRERRIDGLQMIYNVLEQDPGRSLIETAR
                     ETGTSLIVRVPHSSGMLEGHYDENTTFAKNDHRRHRPKEWLTTGLKKVEQLGFLTESG
                     ERTLGQAALKFVLAAPEIVSTLPNIYGEEQIEEFAAAPDTPDLSAEELSRIAELYDSN
                     FGLNEQAAFSSQQPVTES"
     gene            749..1471
                     /locus_tag="AC16CM_15505"
     CDS             749..1471
                     /locus_tag="AC16CM_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR3141287.1"
                     /translation="MTETATRQQQAQEEKLPAQYINYVFFALDPLWRRLSDEEREHGK
                     REFLEAAEQHSAEMLLRSFSLMGLRADADFMLWRIGYDLDAFEGMTAALLNTGLGKYL
                     RVSYSYFALSRRSVYVGEHTPGFENRTYIVPGEGEYLFVYPFVKTRSWYRLPMEERQR
                     MMNEHMQIGRKHYPVKNNTGYCFGIDDYEFILSFEAESPEKFQDLMMELRESEASAYT
                     ELDTPIFTCRRRDLPEILRNLG"
     gene            1634..2557
                     /locus_tag="AC16CM_15510"
     CDS             1634..2557
                     /locus_tag="AC16CM_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3141288.1"
                     /translation="MITRINRRAGENITELVGNTPLLALPSISREVPGVSILGKAEWH
                     NPGGSVKDRPALWMIRDGERSGALTPGKTILDATSGNTGIAYAWIGAALGYEVKLCMP
                     KNASEERKKILKAYGVDFVLTDPGEGSDGAIREARRLYAEDPERYFYPDQYSNPANPR
                     AHYESTAPEIWEQSAGEITHFVAGLGTSGTFVGTATRLKEYDPSIEVISFEPDSPFHG
                     LEGMKHMESAIVPSIYDPTMADRNLATQTEEAYEMVKQVAREDGILIGISAGAALATA
                     LRVARDLDSGTVVTILCDGADKYLSESFWED"
     gene            2559..2978
                     /locus_tag="AC16CM_15515"
     CDS             2559..2978
                     /locus_tag="AC16CM_15515"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3141289.1"
                     /translation="MPLKIGAGDVGLIHEHAVEAYPEECAGALVGIDSGGVRVVVDVW
                     RAENTHEEERSRRFLIEPLAVKKFEERAEEKDMGLLGFYHSHPDHPAEPSEYDREHAW
                     PGYSYVIASVSGENVEDMRSWTLRSDRSGYDEETIVG"
     gene            2991..3272
                     /locus_tag="AC16CM_15520"
     CDS             2991..3272
                     /locus_tag="AC16CM_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR3141290.1"
                     /translation="MPKVKIPTPLRQYTAGSPEVEVGGATAGEALGNLASEHPNLRQH
                     LYTGDGKLRSFVNVYKGDEDIRYLEGPDTEVAEGDELSIIPSIAGGRCR"
     gene            3363..>4336
                     /gene="moeB"
                     /locus_tag="AC16CM_15525"
     CDS             3363..>4336
                     /gene="moeB"
                     /locus_tag="AC16CM_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3141291.1"
                     /translation="MELSNEEIARYSRHLIMPEVALDGQKQLKAAKVLTVGTGGLGSP
                     LALYLAAAGVGTIGIVDFDVVDESNLQRQIIHGTSDVGRPKVQSAYDKLKDINPNVEV
                     RVHEEALTSENALEIFEDYDVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQA
                     SVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAVGTIQATETVKLILGIGEP
                     LIGRLLLYDALGMGFREMKLRKDPNCPVCGENPTVTELIDYQEFCGIPQANAAEAENG
                     VPEITVKELKSKLDNEEDINVLDVREPHEYEVANIGVRLIPLGELPQRL"
BASE COUNT          931 a         1350 c         1345 g          710 t
ORIGIN      
        1 actggtcgga ggaatttatc cgcttcgccc tggagaagag cctcgagagg ctcgacacgg
       61 actacgttga tttcctacag cttcacaaca ccaagatgga cgccgtcgag gacgacgacc
      121 tgttcgccct catggagaag ttcaaggagg agggcaagat ccgggcctac ggcgtcgccc
      181 tgggacccaa gatcggctgg ctcgatgagg gcgtcagggc catgcgtgag cgccggatcg
      241 acggcttgca gatgatctac aacgtcctcg aacaggatcc gggccggagc ctgatcgaaa
      301 cagcccgcga gacgggcacc tccctgatcg tgcgcgtccc ccactcctcg ggcatgctgg
      361 agggccacta cgacgagaac accacgttcg ccaagaacga ccaccgccgc caccgcccca
      421 aggaatggct gaccaccggc ctcaagaagg tcgagcaact gggcttcctc accgaatcgg
      481 gagagcggac cctcggccag gccgccctga aattcgtgct cgccgccccg gagatagtct
      541 ccaccctccc caatatctac ggcgaggagc agatagaaga gttcgcagcg gcccccgaca
      601 cccccgacct cagcgccgag gagctctccc gcatagccga gctgtacgat tcgaacttcg
      661 ggttgaacga gcaggcagcg tttagctctc agcaaccagt gactgagagc tgatggctga
      721 aagccgttac gaccgaaggg agtaacaaat gaccgagacg gcgacacgcc agcaacaggc
      781 gcaagaagag aagcttcccg cccagtacat caactacgtg ttctttgctc tcgatcccct
      841 gtggcggcgt ctgtccgatg aggagcggga gcacggaaag cgtgagttcc tggaggcggc
      901 ggagcaacac tcggcggaga tgctcttgcg gtcgttctcg ctgatgggcc taagggctga
      961 cgcggacttc atgttgtgga ggatcggcta cgacctagac gccttcgagg gcatgaccgc
     1021 ggccctgctc aacacgggcc tcggaaaata tctgcgcgtc tcatactcct acttcgccct
     1081 gagccgccgc tccgtctacg ttggcgaaca tacgccgggc ttcgagaacc gcacgtacat
     1141 cgtgccggga gagggagagt accttttcgt ctaccccttc gtaaagacgc ggtcgtggta
     1201 ccggttgccg atggaagaac gccagcgcat gatgaacgag cacatgcaga tcgggcgcaa
     1261 gcattacccc gtgaagaaca acacgggcta ctgcttcggg atagacgact acgagttcat
     1321 cctctccttc gaggcggagt cgcccgagaa attccaggac cttatgatgg agctcaggga
     1381 gagcgaggcg agcgcctata cagagctcga cacccctatc tttacctgcc gccgccgcga
     1441 cctgccagaa attttgcgga atctgggcta gatcggcgtc cccactctgg aatcgcacca
     1501 cggggttcat gtataatgcc gcgcaacgca ccaaccatgg ccgggaggct ctatctagcc
     1561 tctcggttat ttcgttggtt agacattctc ccgtctggac gacaaaccct caccctgtat
     1621 aatcaatccc gacatgatta ctcggattaa tcggcgagcg ggggaaaaca taacggagct
     1681 tgtcggcaac acgccgcttc tggcgttgcc gagcatctct cgggaggttc caggcgtcag
     1741 catcctcggc aaggcggagt ggcacaatcc tggtggatcg gtcaaagaca ggccagcgct
     1801 gtggatgatc cgggacggcg agaggtcggg cgccctcacg ccgggcaaga ccatcctgga
     1861 cgcgaccagc ggcaacacgg gcatagctta cgcctggatc ggggccgcgc tcggctacga
     1921 ggtaaagctc tgcatgccga agaacgccag cgaggagcgg aagaagatcc tcaaggccta
     1981 cggcgtggac ttcgtgttga ccgatcctgg cgagggctcg gacggggcta tcagggaggc
     2041 gcggcgcctc tacgcggagg acccggagcg gtacttctac cccgaccagt actccaaccc
     2101 ggccaacccg cgcgcccact acgaatccac ggcaccggag atctgggagc agagcgccgg
     2161 tgagatcacg cacttcgtcg ccgggctcgg cacgagcggc acctttgtgg ggacggcgac
     2221 gcggctcaag gagtacgacc caagcataga ggtcatctct ttcgagccgg actccccctt
     2281 ccacgggctt gagggcatga agcacatgga gagcgccatc gtgccctcca tctacgaccc
     2341 gaccatggcg gaccggaacc tcgccacgca gaccgaggag gcctacgaga tggtcaagca
     2401 ggtggcccgc gaggacggca tcctgatcgg catctcggcc ggcgcagcac tcgcgacggc
     2461 gctacgggtc gcgagggacc tggattccgg caccgttgtc accatcctgt gcgacggtgc
     2521 agacaagtac ctctccgaga gcttctggga ggattagctt gccgctcaag ataggcgcgg
     2581 gggacgtcgg tctcatccac gagcacgctg tggaggctta tccggaggag tgcgccgggg
     2641 cgctagtagg catagacagc ggcggcgtca gggtggtcgt ggacgtgtgg cgggccgaga
     2701 acacgcacga agaggagcga agcagaaggt ttctcatcga gccgctcgcg gtaaagaagt
     2761 tcgaggagcg ggcagaagag aaggacatgg gactcctagg gttctaccac tcccacccgg
     2821 accatccggc cgagccctcc gagtacgaca gggagcacgc atggcctggc tactcctacg
     2881 taattgcctc cgtaagcggc gagaacgtcg aggatatgcg ctcgtggacc ctccggagcg
     2941 accgctccgg ctacgacgaa gagaccatag taggataagg agattaggat atgccgaagg
     3001 tcaagatccc cacaccatta agacagtaca ctgccggcag ccccgaggtc gaggtgggcg
     3061 gtgcgacggc cggggaggct cttggcaacc tcgcctccga gcatcccaac ctcaggcagc
     3121 acctgtacac gggcgacggc aagctcaggt ccttcgtcaa cgtctacaag ggcgacgagg
     3181 acatccgtta cctagaaggc cctgacacgg aggtcgctga gggcgacgaa ctctccataa
     3241 tcccctccat cgccggcgga cgatgtaggt agcctttccc gccaggttct cccgagtttc
     3301 aaacagacga ggtttcgaaa atgcaggcaa gacagccact tgtagagtct cccaatggca
     3361 ccatggagtt gagcaatgag gagattgctc gctacagcag gcacctcatc atgccagagg
     3421 tggccctcga cgggcagaag cagctcaagg ccgcgaaggt tctgaccgtt ggaaccggcg
     3481 ggctcgggag cccgctcgcg ctctatctcg cggcggcggg cgttgggacc atcggcatcg
     3541 tggacttcga cgtcgtcgac gagtcgaatc tgcagcgtca gatcatccac ggcacctccg
     3601 acgtgggacg tcccaaggtc cagagcgcct acgacaagct caaggacata aaccccaacg
     3661 tcgaggtcag ggtccatgag gaggctctga cgagcgagaa cgcgctggag atcttcgagg
     3721 actacgatgt gatcgtggac ggcacggaca acttcccaac gcgttacctc gtgaacgacg
     3781 cgtgcgtgct ccttggcaag cccaacgtct acggttccat cttccgcttc gagggccagg
     3841 ctagcgtgtt ctacgccgag gaaggcccgt gctaccgctg cctctacccg gagccacccc
     3901 ctcccgggct cgtgccgagt tgcgcggagg gcggggtgct cggtatcctt cccggtgcgg
     3961 taggaaccat ccaggccacc gagaccgtga agctcatact aggcatcggc gagccgctca
     4021 tcggcaggct cctgctctac gatgcccttg gcatgggctt ccgtgagatg aagctccgca
     4081 aggatcccaa ctgccccgtc tgcggcgaga accccaccgt gacggagctc atcgactatc
     4141 aggagttctg cggcatcccc caggccaacg ccgccgaagc ggagaacggc gtgcctgaga
     4201 tcacggtcaa agaactcaag agcaagctgg acaacgaaga ggacatcaac gtgctcgacg
     4261 tgcgcgagcc acacgagtac gaggtcgcca acatcggcgt caggctcata cccctcgggg
     4321 aactgccgca gcgcct
//
LOCUS       DBNGVD010000576         1919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1247788, whole genome shotgun sequence.
ACCESSION   DBNGVD010000576 DBNGVD010000000
VERSION     DBNGVD010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1247788"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="AC16CM_15530"
     CDS             <1..289
                     /locus_tag="AC16CM_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141292.1"
                     /translation="FLPLCPPTADRPFVERPLMGYAGSTVLADLLDGALREKKRKPSG
                     EAGIRWTDSALQEVAGVPSLLRGRARRLAEDRAREQGSGEVTREIFLESRS"
     gene            309..605
                     /locus_tag="AC16CM_15535"
     CDS             309..605
                     /locus_tag="AC16CM_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141293.1"
                     /translation="MGIRDFIEHLTKHQERLRRRADLSNSEREETLIEAETMVGMADA
                     AIRELGSVLDNRRINLPEEHAVRFAQDVFIYLRDAEEGVKEALRDTTPSNPTGA"
     gene            736..1839
                     /locus_tag="AC16CM_15540"
     CDS             736..1839
                     /locus_tag="AC16CM_15540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3141294.1"
                     /translation="MARRSRKNTAALAIALAVVVLAGCSQGQPVAEDNNNAADVSSGA
                     SAASPRIPSDEPVAIVASDVGPSVVQVNVRAVQETPFGTQKGEGVGSGVIYRPDGYII
                     TNNHVVADATAVNVAFADGTTERASVVGSDPNTEIAVLEVDRDGLPAATFDENTPVVG
                     QLAVAIGSPSGFESTVTSGIVSGVGREFPPELIGSGSEARALVDLIQTDAAISPGNSG
                     GALADRDGRIVGINVAYLPPAETGAENLGFAIPSDTAVSVADQLIDTGRVSTAYLGVV
                     TTDLSPEDAREFDLPVDSGALVEQVVQDSAAADAGVRKGDIITALGRVRVERYGDLLG
                     ALRDYAPGDTVTLTIFRDGDKRKLEATLGEKSG"
BASE COUNT          360 a          623 c          644 g          292 t
ORIGIN      
        1 cttcctcccc ctctgtcctc ccaccgccga caggcccttc gtggagaggc ccctgatggg
       61 ctacgccggc tcgaccgtcc tcgccgacct cctggatggt gcgctccgcg agaagaaacg
      121 gaaaccctcg ggagaggccg gcatccggtg gaccgacagc gcgctgcagg aggtcgccgg
      181 agtaccgtcg ctcttgcggg gtagggccag gcggctggcc gaggatcgcg cgcgcgagca
      241 gggctcgggc gaggtgacgc gtgagatctt cctggagtcc cgctcctgaa cggatataat
      301 cctgccccgt gggcatcagg gatttcatag agcacctcac gaaacaccag gaacggctga
      361 ggcgcagggc tgacctttcg aactccgaac gcgaggagac cctgatcgag gccgagacta
      421 tggtggggat ggccgacgcg gccatcagag aactcgggtc tgtgctagac aaccgccgca
      481 taaaccttcc tgaagaacac gcggttcgct tcgcccagga tgtcttcatc tacctgaggg
      541 acgcggagga aggcgtgaaa gaagccctcc gcgatacgac gccaagcaac cccacggggg
      601 cgtagcgcta gtagggacgc agctcgtgag ccccttcgcc ctccagggtg gccgttcacg
      661 ccggagaggc gcaacctcgg tatagactct gtggcatacg acgcgcccct ggcgcccaca
      721 ggaggttctg acacgttggc tcgtagatcc aggaagaaca ccgcggcgct cgctatcgcg
      781 ctcgccgtgg tagtgctggc aggctgttct caggggcagc ccgtagcgga ggacaacaac
      841 aacgcggccg acgtttcgtc cggggcatcg gcggcttccc cccgcatacc gtcagatgag
      901 ccggtggcga tcgtcgcctc ggacgtggga ccaagcgtgg tacaggtcaa cgtcagggcg
      961 gtgcaggaga cgccttttgg tacgcagaag ggcgagggcg tggggagcgg tgtgatctac
     1021 cgccccgacg gctacatcat caccaacaac cacgtcgtgg ccgacgccac ggccgtgaac
     1081 gtggccttcg ccgacggcac cacggaacgc gcgagcgtgg tcggctcgga cccgaacacc
     1141 gagatagccg tgctcgaggt ggaccgcgac ggcctgccgg cggccacctt cgacgagaac
     1201 acccccgtcg ttggccagtt ggccgtggcg atcggcagcc cctctggttt cgagtcaacg
     1261 gttacctcgg gcatagtcag cggcgtgggc cgggagtttc cgccggagct catcggaagc
     1321 ggatcggagg cgagggccct ggtggacctg atccagaccg acgccgccat ctccccgggt
     1381 aactcgggcg gcgccctggc cgaccgggat ggccgtatcg taggcatcaa cgtggcgtac
     1441 ctgcccccgg ccgagaccgg cgccgagaac ctcggcttcg ccatcccctc agataccgcg
     1501 gtatccgtgg ccgaccagtt gatcgacacg ggcagggtct cgacggctta tctcggcgtc
     1561 gttaccaccg accttagtcc cgaggacgcc agagagttcg accttcccgt cgactcgggg
     1621 gcgctggtag agcaggtggt gcaggacagc gcggccgccg atgcaggggt caggaagggc
     1681 gacatcataa ccgcccttgg cagagtcagg gtggagaggt acggcgacct tctcggagct
     1741 ctgcgtgact acgcgcctgg tgatacggtc accttgacca tcttccgcga cggggacaag
     1801 agaaaactgg aagcaaccct cggcgagaaa tccggctagg ccggaccacc aggctcgtga
     1861 gcgcaacacc gacgggccgc cacacacctc cggcggtaaa ataacgtcat gggacacca
//
LOCUS       DBNGVD010000577         5595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_701302, whole genome shotgun sequence.
ACCESSION   DBNGVD010000577 DBNGVD010000000
VERSION     DBNGVD010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_701302"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="AC16CM_15545"
     CDS             <1..737
                     /locus_tag="AC16CM_15545"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3141295.1"
                     /translation="TLGLVAIAVALSLYERLDLEKDIGVAVVRSFVQLAAIGYVINYV
                     FGLENLWVVVMLLAGMVLFAAWTSARRARDVPNAFPIAVVAVGVAAAATLVLLLALGI
                     VPATARYLIPLGGMVIGNSMNASSLTMTRIRDDLAEQRLKVEAALALGATSRQAVSPL
                     LKTALQRALIPLIDATKTTGIIFLPGAMVGMIIAGADPLEAVRLQIVVLYMLLGSVSI
                     AAILVGTLSYRSFFTRRHQLEAPPEH"
     gene            complement(893..1369)
                     /locus_tag="AC16CM_15550"
     CDS             complement(893..1369)
                     /locus_tag="AC16CM_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141296.1"
                     /translation="MMEGDLSIFPTHILLATDGSPHADLAAGVAVDLARSTGSRLHVV
                     AVGRTFPAAVYDEYAESRREDLRREAQEILDEQVRKIEESGGDVAIAHLKFDEARDEA
                     IVHLAEDIGAGMIVIGSRGFGGMKRALMGNVADSVVRHAHCPVLVVRPSGGSSKPF"
     gene            complement(1401..2150)
                     /locus_tag="AC16CM_15555"
     CDS             complement(1401..2150)
                     /locus_tag="AC16CM_15555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3141297.1"
                     /translation="MNGGRIAGRVAVVTGASSGIGAAVARALCAEGAHVALAARREEV
                     LLDVQASLGDEAGQESIVVPTDVTDRGQVLSLIERAGELGPVEVLVNCAGVMYYTLMK
                     NVHEEEWERTVEVNCKGALNCVGAVLPGMLERGSGHIVTISSDAGRKVFPGLSVYSAS
                     KFFVEALSQGLRLETAGTGLRVTTVQPGNVATDLHSMSTDEEAIREYAGDSGARMLDP
                     EDVAASVVHALVQPEHVAVNEVLIEPSDEPV"
     gene            complement(2188..2793)
                     /gene="cysC"
                     /locus_tag="AC16CM_15560"
     CDS             complement(2188..2793)
                     /gene="cysC"
                     /locus_tag="AC16CM_15560"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3141298.1"
                     /translation="MVGTSKGENMNEERGFTLWFTGLSGSGKTTISEIVERELRNRER
                     PVEVLDGDIVRTNLSKGLSFSREDRNINVLRIGFVANLLTRNGVGVVVSAISPYKEAR
                     DQVRRRIVDFVEVFVDAPLEVCADRDVKGLYKKAFAGEIEQFTGVSDPYEPPNAPDLV
                     LKTDEETPEESAQKVIEKLEFFGYLWPREAQENTEYNWSST"
     gene            complement(2844..4145)
                     /locus_tag="AC16CM_15565"
     CDS             complement(2844..4145)
                     /locus_tag="AC16CM_15565"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3141299.1"
                     /translation="MPQLPNILYIHSHDTGRWVRPYGYSVRTPNIQRLAEEGVLFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLKDYRHHIVHTLRELGYWSALIGE
                     QHISKQPDVIGYDEVVKISTSHTDDVVPVTLDLLERASDRPFFLSVGFFETHREWFRP
                     SSPKKANYVMPPSNLPDAPETRLDMAAFQESARSLDRGVGAVLDALDAEGLAEDTLVI
                     CTTDHGLAFPGAKATLYDRGLGVMLILRGPGGFSGGRASDALVSHIDLFPTICDLTGI
                     DPPEWLQGESLLPLARGEAEEVRDAIFAEKTYHVAYEPERCVRTHRWKYMKRFGERTR
                     PVLPNTDDGPSKDLLLSHGWGEREIPREQLYDIVLDPNEADNLAGSPAHADTLEELRG
                     RLEAWMVETKDPLLDGDVPAPEGAELNDPDGLSPAEPTIVV"
     gene            complement(4148..5029)
                     /locus_tag="AC16CM_15570"
     CDS             complement(4148..5029)
                     /locus_tag="AC16CM_15570"
                     /inference="COORDINATES: protein motif:HMM:NF020604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HIR3141300.1"
                     /translation="MNGRSGPDLSYLICATPRSGSTLLCAALDGTGVAGHPEEHFEVL
                     LETGRRRQPRDYFQRSNDPEVWALLDDPEFREVLGEYGGRYSEHPGERDPDWSPPDFQ
                     KLVREALEKGTTENGVLGTKIMWAYFRDFVRLARRRGLEDVSPCEVPAAVLPGLRRYV
                     WMRREDTTRQAVSLWKALQTQKWRKDSDEEVGGQGLRFSFAAVNHLKLRIDEHNAAWG
                     DYFRDCGVEPVEVSYEELVADYEATVSWLLEEIGISVPEGFAVEAPRMRRQADELSEE
                     WVRLYNDGSLLRTAQKG"
     gene            5239..5403
                     /locus_tag="AC16CM_15575"
     CDS             5239..5403
                     /locus_tag="AC16CM_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141301.1"
                     /translation="MKWELDLWYELWDRAGSQGLDTWYDVKGEGDEGPAVVSNAEGLD
                     EISDRPSEGA"
     gene            5400..>5595
                     /locus_tag="AC16CM_15580"
     CDS             5400..>5595
                     /locus_tag="AC16CM_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141302.1"
                     /translation="MNGPLEKGRSGGWRLGEVSGSAVLLALLFSEVASLVTGMGALYF
                     ASIVAAWLVLPAVVKFENVLD"
BASE COUNT          938 a         1840 c         1741 g         1076 t
ORIGIN      
        1 tgaccctggg cctcgtggcc attgctgtgg ccctgagcct gtacgagagg ctcgacctgg
       61 agaaggatat cggggtagcc gtggttcgct ccttcgttca gctcgccgca atagggtacg
      121 tcataaacta tgtcttcggg ctcgagaacc tgtgggtggt ggtaatgctc ctcgccggca
      181 tggtcctctt cgccgcctgg acctcggcca gaagggcaag ggacgtgccg aacgccttcc
      241 caatcgccgt cgtcgccgtt ggggtggccg ccgcggccac gctggtgctg ttgctcgcgc
      301 tcgggatcgt gccggccacg gcacggtacc tgatcccgct cggcggcatg gtgataggaa
      361 actccatgaa cgcctctagc ctgaccatga cccgcatccg cgacgacctg gccgagcaga
      421 ggctaaaggt cgaggccgcc ctggccctgg gagccacgag ccgccaggcc gtctctcccc
      481 tcctcaagac cgccctccaa cgcgccctca ttcccctcat agacgccacg aagaccacgg
      541 gaataatctt tctgccgggg gccatggtcg gcatgatcat cgccggggcc gacccgctcg
      601 aagcggtgcg cctccagata gtggtcctct acatgctgtt gggcagcgtc tccatagccg
      661 cgatcctggt cggaacgctc tcctaccgct ccttcttcac ccgacgacac cagctcgagg
      721 cgcctcccga acattagagc cttcagcttt cagcagtcag ccgatagccc tcttttccgc
      781 gtttttcctg gccgagctct tcccggaaat gccggcagaa agacgcaagc tccccgcaag
      841 aaagctgacc gctgaaagct aatagctgaa agctaaaaga aagctgtcag ctctaaaacg
      901 gcttgctgga accaccggat gggcgcacca cgaggacggg gcagtgggcg tggcggacca
      961 cggagtcggc gacgttgccc atgagggccc gcttcatgcc cccgaagccg cggctgccga
     1021 tcacgatcat gccggccccg atgtcttcgg cgaggtggac tatggcctcg tccctcgcct
     1081 cgtcgaactt caggtgggcg atcgcgacgt ctcccccgga ctcttctatc ttcctgacct
     1141 gctcgtccag tatctcctgg gcctcacgcc tgagatcctc acgccgggac tcggcgtatt
     1201 cgtcgtagac ggcggctgga aaggtgcgcc cgaccgccac gacgtgcaac cgcgagccgg
     1261 tgctcctggc gaggtcaaca gccaccccgg cggcgagatc tgcgtgcggg gatccgtcgg
     1321 tggccagtag gatatgcgtc ggaaagatac tcaaatcacc ctccatcatg gtaccgcctt
     1381 attccggcac caaaacatgc ctagaccggc tcgtcgctgg gctcgatgag gacctcgttt
     1441 acggctacgt gctctggctg cacgagggcg tgcaccaccg aggcggccac gtcctcgggg
     1501 tctagcatgc gcgcccccga atcgccagcg tactcccgga tcgcctcctc gtcggtgctc
     1561 atggagtgca ggtccgtggc cacgttgccg ggctgaacgg tcgtcaccct tagccccgtg
     1621 cccgcggtct cgagccgcag cccctgcgag agcgcctcca cgaagaactt gctcgccgag
     1681 tagaccgaga ggccggggaa gaccttgcgc ccggcgtcgg aggagatggt gacgatgtgc
     1741 ccgctacccc tctctagcat ccccggcaac acggcgccca cgcagttgag cgcgcccttg
     1801 cagttgacct cgacggtccg ctcccactct tcctcgtgga cgttcttcat cagcgtgtag
     1861 tacatgacgc cggcgcagtt gacgagcacc tcaacggggc cgagctcccc ggcgcgctct
     1921 atcagggaga gcacctgccc gcggtccgta acgtcggttg gaacgacgat gctctcctgg
     1981 ccggcttcgt ccccgaggct ggcctgaacg tcgagcagca cttcctcacg cctggccgcg
     2041 agcgcgacgt gggcgccctc ggcgcagagc gcccgtgcca cggcggcgcc gatgccgctc
     2101 gacgcccccg tcaccacggc aaccctgccg gcgatccgcc cgccgttcat cggcgggccg
     2161 acaggccgaa gttccgacca gagtttttca ggtcgagctc cagttgtatt cggtgttctc
     2221 ctgggcctcc ctgggccaga ggtacccgaa gaactccagc ttctctatca ccttctgggc
     2281 gctctcctcg ggcgtctcct cgtcggtctt tagcacgagg tcaggggcgt tgggtggctc
     2341 gtacgggtcg gagacaccgg tgaactgctc gatctccccc gcgaaagcct tcttgtaaag
     2401 gcccttcaca tccctgtcgg cgcagacctc gagcggggcg tcgacgaaga cctcgacgaa
     2461 gtcgacgatc ctgcgccgca cctggtcccg cgcctccttg taaggcgaga tggcgctaac
     2521 gaccacccca accccgttgc gcgtgagcag gttggcgacg aaccctatcc tcaagacgtt
     2581 tatgtttcgg tcttccctgg agaagctaag gcccttggag aggttggtcc tgacgatgtc
     2641 cccgtcgagc acctccacgg gccgctccct gttcctgagc tcgcgctcga cgatctcgga
     2701 gatggtggtc ttgcccgacc cggagaggcc ggtgaaccac aacgtgaaac cgcgttcttc
     2761 gttcatgttt tctcctttcg acgttcctac gatgtttgcg gacgtagttt acatatacct
     2821 tcgcgctctg ctagcggcac ggctcatacc acgatggtgg gttcggcggg ggagagaccg
     2881 tctggatcgt ttagctcggc cccctcgggt gcaggcacgt ccccgtcgag gagcgggtcc
     2941 ttggtctcga ccatccaggc ttcgagccgt ccgcgcagct cctcaagagt gtccgcgtgg
     3001 gcagggctcc cggccaggtt gtccgcctcg ttcgggtcga gcacgatgtc gtagagttgc
     3061 tccctgggta tctcgcgctc tccccagccg tgggagagca acaggtcctt gctcggcccg
     3121 tcgtcggtgt tgggtagaac cggcctggtg cgctccccga agcgcttcat gtacttccag
     3181 cggtgggttc gcacgcaccg ctccggctcg taggccacgt ggtaggtctt ctccgcgaag
     3241 atggcgtccc tgacctcctc agcctcgccg cgtgcgagcg ggagcagcga ctcgccctgc
     3301 aaccattcag gcgggtctat gccggtgagg tcgcagatag tggggaagag gtcgatgtgg
     3361 gagacgagcg cgtcgctggc cctaccccca ctgaacccgc cggggccacg caggataagc
     3421 atgacgccga gccccctgtc gtagagggtg gccttggcgc cagggaaggc gaggccgtgg
     3481 tctgtggtgc agatcacgag cgtgtcctcg gcgagcccct ctgcgtcgag ggcgtcgagc
     3541 acggcgccca cgcccctgtc gagcgagcgg gcgctctcct ggaaggcggc catgtcgagg
     3601 cgcgtctcgg gtgcatctgg caggttggag gggggcataa cgtagttggc tttcttcggc
     3661 gagcttggac ggaaccattc gcggtgggtc tcgaagaacc ccacggaaag gaagaagggc
     3721 ctgtcgctcg cgcgctccag caggtcaagg gtgacgggca ccacgtcatc ggtgtggctg
     3781 gtgctgatct tgaccacctc gtcgtaccct attacatcgg gctgcttgga gatgtgctgc
     3841 tcgccgatga gggccgacca gtacccgagc tcgcgcaggg tgtgaacgat gtggtgcctg
     3901 tagtctttga gcgagaaacc gcggtgggcc aggccgacca tgccgttgga gtgggcgtac
     3961 tggccggtca gcagacacgc ccggctcccc gagcacgtcg gggccgcgca gaaggccttg
     4021 cggaagagca caccctcctc tgcgagacgc tggatgttcg gggtcctgac ggagtagccg
     4081 tacggcctga cccaccgtcc cgtgtcgtgg gagtggatgt agagtatgtt cggaagctgg
     4141 ggcaccttta tcccttctga gcggtcctga gcagggagcc atcattgtac agacgtaccc
     4201 actcctcgga gagctcgtct gcctgccgcc gcatcctggg cgcctcgacc gcgaagcctt
     4261 ctggaacgga gattcctatc tcctcgagga gccacgacac agtggcctcg taatccgcca
     4321 cgagctcctc gtaggacacc tctacgggct ccacgccgca atctcgaaag tagtcccccc
     4381 aggcggcgtt gtgctcgtcg atgcgcagct tgaggtggtt cacggcggcg aagctgaacc
     4441 gtagcccctg cccgccaacc tcctcatcgg agtccttgcg ccacttctgg gtctgaagag
     4501 ccttccacaa cgagacggcc tgcctggtgg tgtcctctcg gcgcatccac acgtaccgac
     4561 gcaggcccgg cagcacggcc gctggaacct cgcaaggact cacgtcctcc agaccacgcc
     4621 tgcgcgccag acgcacgaag tccctgaaat acgcccacat gatcttggtc ccaagcaccc
     4681 cgttttctgt ggtgcccttc tccagcgcct ccctgaccag cttctggaag tcgggcgggc
     4741 tccaatccgg gtccctctct cccggatgct cggaatacct gcccccgtat tcgccaagaa
     4801 cctccctgaa ctccggatcg tccagcaacg cccacacctc ggggtcgttt gagcgctgga
     4861 agtagtcgcg cggctgcctc cgccgccccg tctcaagcaa gacctcgaag tgctcctccg
     4921 gatggcccgc cacccccgtg ccgtctagcg ccgcacacaa cagcgtgcta ccactccgcg
     4981 gcgtcgcaca gatcaaatac gaaaggtccg gtccggatct accgttcatg atgcctcctg
     5041 gtgatagggt aatctatgtc aaacctatct tgtctattgc gatggtaaag aatacacgaa
     5101 accggttcgg gatcaatgcc cccacccgtc tcttctgatt ggaaagtacc caggaatagg
     5161 ccacagcgct tgacgctgcg ggcatggcta tataccataa tgtctactta taaagtaaac
     5221 aaaggtagag gttggcgcat gaagtgggag ttggatctct ggtacgagtt gtgggacagg
     5281 gctggaagcc aggggttgga tacctggtac gacgtcaagg gcgagggaga tgaggggcct
     5341 gcagtggtgt cgaatgcgga gggcctggat gagatctccg atcggccttc tgagggtgct
     5401 tgaacggtcc tctggaaaag gggcgtagcg gtggctggcg gctcggtgag gtctccggtt
     5461 cggcggtgct gttggcgttg ctcttctcgg aggtggcatc cctggtaacg gggatgggcg
     5521 cgctctactt cgcgtccatc gtggcggcct ggctcgttct accagccgtc gtgaagttcg
     5581 agaacgtcct cgatg
//
LOCUS       DBNGVD010000578         2801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4527262, whole genome shotgun sequence.
ACCESSION   DBNGVD010000578 DBNGVD010000000
VERSION     DBNGVD010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4527262"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="AC16CM_15585"
     CDS             <1..179
                     /locus_tag="AC16CM_15585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3141303.1"
                     /translation="VARLGRSSVRYEIGLFRNDDDTAAAQGHFVHVYVERETRKATTL
                     PPEIRAALGKLLVS"
     gene            complement(336..1040)
                     /locus_tag="AC16CM_15590"
     CDS             complement(336..1040)
                     /locus_tag="AC16CM_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR3141304.1"
                     /translation="MMTEKIGPVQLLAVGFDPDARFEGKILDELESLERHETIRILDL
                     LFVGKDAETGDLLALDYQGEELGAIVGALLGFEFEDDGAGPDGLLEEIQAHAFGLSQT
                     EIQDIAASLHPGDSAGFVLIEHVWARDLKGAIREAGGFPLGEGFLTPEALAGVATELV
                     AMSEAIEEIEVEEAGGGRRGYGGFNTRPPRTRKSRGCSSREPNSHLARKLQSFRAHRS
                     RLSRPIPDVRKASNAS"
     gene            complement(1132..1419)
                     /locus_tag="AC16CM_15595"
     CDS             complement(1132..1419)
                     /locus_tag="AC16CM_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141305.1"
                     /translation="MGCLFVLLGGAFPRLALLILWIVRPALVGAAFNTFLLPLLGIIF
                     LPFATLIYVFVYTPGVGLVGFDWFWVILAVLLDIGSWGATYTQRDQIPGTR"
     gene            complement(1687..2250)
                     /locus_tag="AC16CM_15600"
     CDS             complement(1687..2250)
                     /locus_tag="AC16CM_15600"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR3141306.1"
                     /translation="MSTEGRYQQRLSTLADNWRALALRGIVALLFGLVVLFWPGLVLA
                     VLAIFFGIYAALDGAITFVPALRSQERGMKRTLPLAEGVVGIVAGLVAIFWPGLTSSG
                     LVYVIAGWAVVTGALKILTAILLRTEVQNGWLLAGSGGLSALFGVLLIVLAGSDVPFL
                     APFIGVFAVVVGLALIVFAFRLRERRR"
     gene            complement(2264..2776)
                     /locus_tag="AC16CM_15605"
     CDS             complement(2264..2776)
                     /locus_tag="AC16CM_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3141307.1"
                     /translation="MLSETRRWWKMSALSLAFGPMLALVVIVSILLMLIGPDLIERIA
                     ALVGLEEVFVSLWGWLRFPIALSLLAGVLSLIYRYGPNARQRFRSVIPGAVLAVVLWA
                     VTSVGFSFYLATFANYGVTYGSLGAAVGLLFYLYLSASVVLFGAEVNAAIYRPVTNAS
                     TKGRKQQKAI"
BASE COUNT          598 a          899 c          829 g          475 t
ORIGIN      
        1 gcgtcgcgcg cctgggccgt agcagcgtgc gctacgagat cgggctgttc agaaacgacg
       61 atgacaccgc cgcggcccag ggccacttcg tccacgtcta cgtcgagcgc gagacgcgca
      121 aggcgacgac cctgccgccc gagatacgcg cggcgctcgg aaagttactc gtcagctagt
      181 aactgcaggc ccgcttcgta tgaagcgagg cgatacagaa ttacatgttt gacgcgctac
      241 gtgacattct cgtcaacacc agcggcgatg gtgacattcg agagacgtac accgaggaca
      301 cgaccgcact cgcaagggcc acggcgtcgt tctccttacg atgcgttcga tgctttcctg
      361 acgtcgggaa tcggtcgcga caaacgcgaa cggtgcgccc gaaatgactg gagtttgcgc
      421 gcaagatgcg agtttggttc ccgtgaactg catccccgac ttttgcgtgt acggggtggg
      481 cgggtgttga aacctccgta gccccttcgg ccaccgcccg cctcctcaac ctcgatttcc
      541 tctatggcct cggacatggc gacgagctcg gtagcgaccc cggcgagggc ttcgggggtt
      601 aggaaaccct cccctagcgg gaagccaccc gcttcgcgta tggcgccctt cagatcacgc
      661 gcccatacgt gctcgatgag gacgaagcca gccgagtcgc cagggtgcag cgaggccgct
      721 atgtcctgga tctccgtctg tgagagcccg aaggcgtgag cctggatctc ctccaatagt
      781 ccgtccggcc ccgcgccatc atcctcgaat tcgaagccca gcagcgcgcc gactatggcg
      841 ccaagttctt cgccctggta atcgagggcg aggagatccc ccgtctcggc atccttgccc
      901 acgaacagga ggtccagaat gcgtatggtc tcgtggcgct cgagactttc gagctcgtcc
      961 aggatcttgc cctcgaacct ggcgtccggg tcgaatccca ccgccagaag ctgaaccggt
     1021 cctatcttct ctgtcatcat ttagcacctt tgttcgttgg tcgagccacg tgaacactcc
     1081 ctcagcatcg tcgtcccgca cgggcgacga tcgtcccagc gttgttcacc ttcaccgtgt
     1141 gccggggatc tggtctcgct gggtgtaggt ggcaccccag ctaccgatgt cgagcaggac
     1201 ggcgaggatc acccagaacc aatcgaagcc cacaaggccc acaccgggcg tgtagacgaa
     1261 cacgtagatg agggtcgcaa agggcaggaa gatgatgcca agcaaaggga ggaggaaggt
     1321 gttgaacgcc gccccgacaa gggccggacg gacgatccag agtatgagca gagcgagacg
     1381 cgggaaggcg cctccgagta gcacgaatag acatcccatt cttgttgcct cctgtctgat
     1441 cctgcaccgt ccatcgcctc gggaccgccg tgggcgggtc accgatcagg ctgtcctcgg
     1501 gccgtcctca ctgccggtgg ggtgacgatc catggcaaca tccctcggct ccggtttctc
     1561 gtatgcgccc gatctgcatg acacctctgg agcataagcc ccggcacgga ggtgcgtcat
     1621 cacccaggag cgcgtatcac ctaggttata tccaggccac aacgtcaggc aggggctccc
     1681 ggtagatcac cttctgcgct cccgcaagcg gaaggcgaag acgatcaagg ccagaccgac
     1741 caccactgcg aagacaccga taaacggcgc cagaaatggc acgtcggagc cggccagtac
     1801 aatgaggagt acgccgaaga gcgccgacaa gccgccgctt ccggccagca gccacccgtt
     1861 ctggacctcg gtgcgcagaa gtatagctgt gagtatcttg agagcgccgg tcacgacggc
     1921 ccatcccgcg atgacgtaga ccaagccgct tgaggtaagt ccgggccaga agatggccac
     1981 cagtcctgcg acgatgccca ccaccccttc ggctaaagga agcgtcctct tcatgccccg
     2041 ctcctgagat ctgagcgcgg gtacgaaggt gatcgcccca tcgagggccg cgtagatgcc
     2101 aaagaagatc gctagcacag cgagcacgag gccaggccag aagaggacca cgaggccgaa
     2161 aagcaaggct actatgcccc gtagcgccag ggctcgccaa ttgtcggcta gcgtgctcaa
     2221 cctctgctga tatcgccctt cagtactcat ggatgcagcc tccttagatc gctttctgct
     2281 gtttccgtcc ttttgtgctg gcgttcgtga ccggacggta gatcgccgcg ttgacctccg
     2341 cgccgaacaa caccaccgac gcggacagat acaagtagaa gagcaaacca acggctgccc
     2401 caaggctccc gtaggtgacc ccgtaattcg cgaaggttgc caggtagaag gagaacccaa
     2461 ccgaggttac ggcccacaga accacggcca gtacagcgcc cgggatcacc gagcgaaagc
     2521 gctgcctggc attgggaccg tatcggtaga tgagtgagag caccccggct agcaaggaga
     2581 gggctatggg gaagcgcagc catccccaca acgagacgaa cacctcctca agccctacca
     2641 acgccgctat acgctcgatc aaatcggggc cgatcaacat tagcaagatg gagacgatca
     2701 ccacgagtgc gagcatcggg ccgaaggcca gagagagcgc cgacatcttc caccagcgcc
     2761 gcgtctctga cagcatgttt cacgttaact gacccgctcg a
//
LOCUS       DBNGVD010000579         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4893420, whole genome shotgun sequence.
ACCESSION   DBNGVD010000579 DBNGVD010000000
VERSION     DBNGVD010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4893420"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1147)
                     /locus_tag="AC16CM_15610"
     CDS             complement(176..1147)
                     /locus_tag="AC16CM_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3141308.1"
                     /translation="MPLIDNQSTRKLFRGARKNLVRTRRRVTRGLYNLRRRNRPDGGG
                     IPPERLVWIFGSARTGSTWLGAMMADLEDHTWWHEPLVGYLFGHIYYERARGRRGDEN
                     FILGGDKALWLGPVRRFVLDSASVRFPERADGGYLVIKEPHGTLGAPLLSEALPESRM
                     IVLVRDPRDVVSSKMDAARQGSWAEEAQKSRGQEKKNTAADDKPDAFVKTQARRYARL
                     IELAQRAYDAHEGPKVLVRYEDLRAEPLQTMRHIYGRLGIPAEERELARVVEKHSWEN
                     IPDRKKGAGKFYRKGSPGGWAEDLTPEQVETVEVITAPLLERYYAAD"
     gene            1274..>2280
                     /locus_tag="AC16CM_15615"
     CDS             1274..>2280
                     /locus_tag="AC16CM_15615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3141309.1"
                     /translation="MTRRRPGKRVRRAFYVVLLAAALGLALSFWSWVDAQARAVVVIS
                     SVLDSPVLTPFVTAISGDPRLSDTRVAGYPSLVAKPAGEGPWPAVFVVNGAVPEGRKL
                     PAVRQLAKGFARAGYLAVVPDLPGLTEDRITPRTVDAATEVAGAISARPDAEDGEVAL
                     VGVSTGATLALLAAEDSSLEGKVSLVAGVAPFSSIKTVLSVATTAHYRRPDGGLVRYR
                     ADPFLSYVAARSLVATLPRGEDRRTLSDEISGVGREHPDPLHDLRQRPTDDLGPQAKS
                     VVRVLANRNPERFDDLYAALPEGIVHELEELSPLAGGERIGAPVELATGPHDAYFPPS
                     ES"
BASE COUNT          330 a          782 c          750 g          418 t
ORIGIN      
        1 gtccaggtcg ggcagtttct ggccctgctg cggcaagttg gtcatgagag atcctccatc
       61 gtggtgtccg accgagagtc tacgttatta gctgtcaacc atcagctatt agccgtcagc
      121 aacagtagat agctggccgc gcatcgtacg gaggtcttga cccctgcact tcgcgttagt
      181 ctgcagcgta gtaccgctcc aggagcgggg cagtgatgac ctccaccgtc tcgacctgct
      241 cgggggtcag gtcttccgcc cacccgcccg ggcttccctt ccggtagaac ttgccggcgc
      301 ccttcttcct gtctgggatg ttctcccagg agtgcttctc caccaccctg gccagttcac
      361 gctcctcggc cgggatcccg agcctcccgt agatgtggcg catggtctgc aggggctccg
      421 ccctcaggtc ttcgtagcgg acgagcacct tcggcccttc gtgcgcgtcg taggcccgtt
      481 gggcgagctc gatcaaccgg gcgtaccttc tcgcctgggt tttcacgaag gcgtccggct
      541 tgtcgtcggc cgcggtgttc ttcttttcct ggccacggct cttctgcgcc tcctcggccc
      601 aactaccctg cctcgcggcg tccatcttcg aagagaccac atcgcgcggg tccctgacga
      661 gcacgatcat gcggctctcg ggaagggcct cggagagcag cggcgcgccg agggtgccgt
      721 ggggctcctt gatcacgaga taccccccgt ccgcccgctc cgggaacctg acgctggccg
      781 agtcgaggac gaaccttctg acggggccga gccagagggc tttgtcgccg cccaggatga
      841 agttctcgtc cccacgccgg cccctggccc gctcgtagta gatatggccg aagaggtagc
      901 cgacaagggg ctcgtgccac caggtgtggt cttccaggtc ggccatcatc gccccgagcc
      961 aggtgctgcc cgtccgagcc gagccgaaga tccagaccag gcgctctggt gggatccccc
     1021 caccatcggg ccggttgcgc cttctgagat tatagagccc gcgtgtgacc ctgcgccggg
     1081 tccgaacgag gttctttctc gcgccgcgga agagtttccg ggtgctttgg ttgtcgatga
     1141 ggggcatgga agagcctttc tatgtacggg cgtaaggtta gggaaaccgt accatcgtgt
     1201 aaagcggcgg ggtgccggga ggcctcccgg agcgtggaca ggtggtgatc cgcgttgcat
     1261 agttacggat gaggtgacga ggagacgacc cggaaagagg gttcggcgcg cgttctacgt
     1321 cgtgctgctc gccgccgctc tcggcctggc cctctcgttc tggtcgtggg tggacgccca
     1381 ggcgcgtgcc gtggtggtga tctcctccgt cctcgactcc ccggttctca ccccgttcgt
     1441 tacggcgatt agcggggacc cgcgcctctc cgacacgcgg gtcgcggggt acccttcgct
     1501 ggtcgcgaaa ccggcggggg agggaccttg gccggccgtg ttcgtggtga acggggccgt
     1561 gccagaggga aggaagctgc cggcggttcg gcagctggcg aagggtttcg cgcgggccgg
     1621 ctacctcgcg gtcgtgcctg acctgccagg cctgacggag gacaggatca ccccccggac
     1681 cgtggacgcc gccacggagg tcgcgggcgc gatctccgca aggcccgacg ccgaggacgg
     1741 ggaggtggcc ctggtcgggg tctcgaccgg cgcgaccctg gcccttctcg ccgccgagga
     1801 ttcctcgcta gaggggaagg tctccctcgt ggccggcgtc gcgcccttct cgagcataaa
     1861 gaccgttctg agcgtcgcga ccaccgccca ctaccgtcgt cccgacggcg ggctggtgcg
     1921 ctacagggca gaccccttcc tctcctacgt cgcggcccgc tcgctggtcg ccaccctccc
     1981 gcggggcgag gatagacgga ctctttccga cgagatctca ggcgtcggcc gcgaacaccc
     2041 tgacccctta cacgacctcc gccagcgccc gacggacgac ctgggaccgc aagcaaagag
     2101 cgtcgtccgg gtgctcgcca acaggaaccc agaacggttc gacgacctct acgcggccct
     2161 ccccgaggga atcgttcacg agctcgaaga gctctcgccg ctcgcgggag gagagcgtat
     2221 cggcgccccc gtcgagctcg ccacgggccc ccacgacgcg tacttccctc cctccgagtc
//
LOCUS       DBNGVD010000580         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3254865, whole genome shotgun sequence.
ACCESSION   DBNGVD010000580 DBNGVD010000000
VERSION     DBNGVD010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3254865"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="AC16CM_15620"
     CDS             complement(<1..820)
                     /locus_tag="AC16CM_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3141310.1"
                     /translation="MQAVMETYKRLEIAPTGGEGSWLFDEEGNRYLDFIGGIATNSLG
                     HGHPALVEAIREQAGKLIHCSNLYEIPLQAEVAGMMADATDFDRVFFCNSGTESVEAA
                     IKLARKHAHETFGPQKYEVLTFSKSFHGRTYGGLTATGQPALQDGFGPMLPGVVHAPF
                     GDLEAARTMVGPQTAAILVEPIQGEGGINVAPEGFLQGLRDLCDEHGALLVFDEVQTG
                     AGRTGHLYAYQGTGVVPDVLTSAKGLGGGFPVGAMMVREEHAALGPGNHGSTFGG"
     gene            complement(808..1584)
                     /gene="argB"
                     /locus_tag="AC16CM_15625"
     CDS             complement(808..1584)
                     /gene="argB"
                     /locus_tag="AC16CM_15625"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3141311.1"
                     /translation="MSGGVPVVIKVGGGALAGGALEDLPELLSAGHRVAVVHGGGAQL
                     TRMLDALDIPTRFHAGLRVTDEATLEVAETVFAGGVNKNLARWLTGLGVPSAGISGTD
                     GPTLVVEPVPDLGRVGNVVQVETRLIEALWDGGFVPVIAPLGVGPEGAYNVNADTAAA
                     SLAVGLGAGHLFLMTDVDGLLRDGETVETLAPERCEEYVERGFAAGGMVPKLRAAAEA
                     ARGGVEARILNGNKKGVLAAAVAGGHVGTLISEGVAACKQ"
     gene            complement(1581..2753)
                     /gene="argJ"
                     /locus_tag="AC16CM_15630"
     CDS             complement(1581..2753)
                     /gene="argJ"
                     /locus_tag="AC16CM_15630"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3141312.1"
                     /translation="MAAAMGFMAAGVECGVRGERGKDLGLLFSGLPCETAAVFTRNAL
                     KGAPLAVTREAVEAGGVRAVVVNSGIANAATGLGGIEDARAMQELAARELGIEAVEVA
                     VASTGVIGARLPMDRISAGVKAAADGLGGDGFAGAILTTDTREKEAVTQVEIGGKTVT
                     VGGTAKGSGMIHPNMGTMLAFLTTDAAVEKECLGETLRVVTDRTFNRVTVDGDTSPSD
                     MALLMANGAAGNEPLGLDSPDYPAFAGAVESVARELAKEISRDGEGATRLVEVVVEGA
                     AGEREATALAKSIVGSNLVKAAVFGEDANWGRVITAMGYSGVAFDPEGVDLWFVPVKV
                     FSRGEPVPHEEAEANATLAGDEVKITARLGEGDASAVAWGCDLSYEYVRINGSYRT"
     gene            2890..3897
                     /gene="argC"
                     /locus_tag="AC16CM_15635"
     CDS             2890..3897
                     /gene="argC"
                     /locus_tag="AC16CM_15635"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3141313.1"
                     /translation="MGLNVGVYGGSGYAGVELVRLLWGHPEVGGLRVASRGYTGRRIS
                     SVYPELAVEGSYVAPEEIAASDLDVAFVAYGHSESAEAVSALIEAGARLVVDLSADFR
                     LGDAGLYEEWYGEHPAPELLGGAHYGLPEVFGVAEGRLIANPGCYPTAAILALAPVVK
                     RVGGIRSVTINALSGVSGAGAKPSVKTHFVSVNESANAYGAPLHRHTSEIETLLGRVG
                     EVPNVTFVPHLIPITRGELETIVVDLEEVPGVEEVLGWYAEDYDGWAFVEAREEYPHV
                     SHVANTNRCRLSAAVDGRAGKLLLFSAVDNLLKGASGAAVQNMNLALGYEEHLGLEHL
                     T"
     gene            3928..4386
                     /locus_tag="AC16CM_15640"
     CDS             3928..4386
                     /locus_tag="AC16CM_15640"
                     /inference="COORDINATES: protein motif:HMM:NF014864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR3141314.1"
                     /translation="MDKTTRQDLILRLISERDLGTQQDVVGALADENVEVAQATVSRD
                     LAELGVLKVGSRYLALPHDPGSAGIEVLPSFVLGIVPAQNQVVIHTRDGTAGAVSSVL
                     DRVKGLKILGTVAGQDTVLAVAPDADAAAEVANLIADVCRARTRPAGNVE"
     gene            4388..>4573
                     /locus_tag="AC16CM_15645"
     CDS             4388..>4573
                     /locus_tag="AC16CM_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141315.1"
                     /translation="MGSGRSDRAGVQAPVPALTVLGGRPFISRSSSYFDTGHLLPGEE
                     QLELAVGRLRAGDHRVGD"
BASE COUNT          729 a         1581 c         1486 g          777 t
ORIGIN      
        1 taccaccgaa ggtggagccg tggttgcctg gtcctagcgc ggcgtgctcc tccctgacca
       61 tcatcgcccc gaccgggaaa ccgccgccca accccttggc gctggttagc acgtctggca
      121 cgacgccagt cccctggtaa gcgtagaggt gccccgtgcg ccccgcgccg gtctggacct
      181 cgtcgaacac gagcaacgcc ccgtgctcgt cgcacaggtc gcgtagtccc tgcagaaaac
      241 cctcgggtgc gacgtttata cccccctcgc cctggatggg ctccacgagg atggcggccg
      301 tctgcgggcc aaccatggtc cttgcggcct ccaggtcacc gaagggagcg tgtactaccc
      361 caggcagcat ggggccgaag ccatcctgca gggctggctg gccggtggcg gtcaggccgc
      421 cgtaggtgcg gccgtgaaag gacttcgaga aagtcagtac ctcgtacttc tgcggcccaa
      481 aggtctcatg agcgtgcttg cgggcgagct ttatcgccgc ctccacgctc tcggtgccgg
      541 agttgcagaa gaaaacccgg tcgaagtccg tggcgtcggc catcatcccg gcgacctcgg
      601 cctgcagcgg gatctcgtac aggttggagc agtggatgag cttccccgcc tgctccctga
      661 tcgcttcgac gagggcgggg tgcccgtggc cgagggagtt cgtggcgatc cccccgataa
      721 agtccaggta gcggttgcct tcttcgtcga agagccagct cccttcaccc ccggtcggcg
      781 ctatctcgag gcgtttgtag gtctccatta ctgcttgcat gccgcgactc cttcggatat
      841 gagtgtccca acgtgcccgc cggcgacggc cgccgcgagc actcccttct tgttgccgtt
      901 caggatcctg gcctccacgc cgccacgcgc ggcctccgcg gccgcccgca acttgggcac
      961 catcccgccg gcggcgaacc cgcgctcgac gtactcctca caccgttctg gggcgagggt
     1021 ctctaccgtc tcgccgtccc gaagcaagcc gtccacgtcg gtcatcagga acaggtgccc
     1081 ggcccctagc ccgacggcga ggctagctgc ggccgtgtcg gcgttgacgt tgtaggcgcc
     1141 ctccggccct accccgagcg gcgcgatgac gggcacgaac ccgccatccc acaacgcttc
     1201 gatcaacctc gtctcgacct ggacaacgtt acccaccctc ccaaggtctg gaacgggctc
     1261 gaccacgagg gtcggcccgt cggtgccgga gatgcccgcg gacggcacac ccaggccagt
     1321 cagccagcgc gcgaggttct tgttgacgcc gccggcgaac accgtctcgg ccacctccag
     1381 cgtggcctcg tcggttaccc tgagcccggc gtgaaaccgg gtgggaatgt cgagcgcgtc
     1441 cagcatcctg gtgagctgcg cccccccacc gtgcaccacg gcgaccctgt ggcccgccga
     1501 aaggagctcg ggcaggtcct cgagggcgcc gccagcaagg gcgcctcccc cgaccttgat
     1561 caccacgggc acgccgccgc tcacgtcctg tagctcccgt tgatcctgac gtactcgtag
     1621 ctcaggtcgc agccccaggc gacggccgag gcgtcaccct cacccaggcg cgcggtgatc
     1681 ttcacctcgt cgccggcgag cgtggcgttg gcctccgcct cctcgtgcgg cacgggctcc
     1741 ccgcgggaga aaaccttgac cggcacgaac cagagatcta cgccttcggg gtcgaaggcg
     1801 acccccgagt agcccatggc cgtgatgacg cgtccccagt tggcatcctc cccgaagacc
     1861 gcggccttga cgaggttgct ccccacgatg cttttggcga gcgccgtcgc ctcccgctca
     1921 cccgccgcgc cctccacgac gacctcgacg agcctggtgg ccccctcgcc gtcgcgagag
     1981 atctctttgg caagctcccg tgccacgctc tcgacagcac ccgcgaaggc cggatagtca
     2041 ggggaatcga ggccaagtgg ctcgttccca gccgccccgt tggccatgag cagcgccatg
     2101 tcactcgggg aggtgtcgcc gtccacggtg acgcggttga aggtgcggtc cgtcaccaca
     2161 cgcagcgtct ccccgagaca ctccttctcc acggcggcgt ccgtggtcag gaaggcgagc
     2221 atggtgccca tgttggggtg gatcatgccg cttcccttgg ccgtcccccc gaccgtgacg
     2281 gtcttgcccc cgatctcgac ctgcgtcacc gcctcttttt cacgcgtgtc cgtggtcagg
     2341 atggccccgg cgaagccatc gccgcccagc ccgtctgccg ccgcctttac gccggcggag
     2401 atcctgtcca tcggcaaccg tgccccgatg acgcccgtgg aagccacggc tacctccaca
     2461 gcctctatcc caagctcccg cgccgcaagc tcctgcatgg cgcgcgcgtc ctcgatgccg
     2521 cccaacccgg tcgccgcgtt ggcgatgccg ctgttgacca cgacagcccg cacgccacca
     2581 gcctcgacag cctcccgcgt caccgcgagc ggtgccccct tgagcgcgtt gcgcgtgaat
     2641 accgccgccg tctcgcaagg taacccggag aagagaagcc caaggtcctt tcctctttcc
     2701 cccctcaccc cgcactcgac cccggccgcc ataaagccca tcgccgcagc cacgccacga
     2761 gaacgagagt ttattccgat tgttgaatct ttattcatca cgggcgcata ttgtcgcacc
     2821 cgcgtgtggg aaggtcaacc aaaatatgca gaaacttgaa taaatatccg aagctcctac
     2881 aatgggcgca tgggattgaa cgttggagtc tatggtggga gcggttacgc cggggtcgag
     2941 ctggtgcggc tgctctgggg gcaccccgag gttggggggc tcagggtggc gtcgcgcggg
     3001 tacacgggga ggcgcattag cagcgtgtat ccggagcttg ccgtcgaggg ttcttacgtt
     3061 gcgccggagg agatagctgc atctgacctg gacgtggctt tcgtcgcgta cgggcatagc
     3121 gagagcgcgg aggcggtaag cgctctgatc gaggccgggg cccgtctggt ggtggatctc
     3181 tccgcggact tcaggctggg cgatgccggg ctatacgagg agtggtacgg ggagcatccc
     3241 gcaccggagc ttctcggagg ggcgcactac gggttgcccg aggtgttcgg ggttgctgag
     3301 gggaggctca tcgccaaccc gggctgctat ccgacggcgg ccatcctggc tcttgcgccc
     3361 gtagtaaagc gggtgggagg gatacggtcc gtcaccatca acgcgctctc gggggtgagc
     3421 ggggccgggg caaagccaag cgtcaagaca catttcgtta gcgtcaacga gagcgccaac
     3481 gcgtacgggg cgccgttgca ccggcacacg tcggagatcg agacgttgct cgggcgggtt
     3541 ggggaggtac ccaacgtgac cttcgtgccg cacctcattc caatcacgcg cggggagctg
     3601 gagacgatcg tggtggatct ggaagaggtg cctggcgtag aagaggtgct cggctggtac
     3661 gcggaggatt acgacggctg ggcctttgtc gaggcgcggg aggagtaccc gcacgtctcg
     3721 cacgtggcca acaccaacag gtgcaggctc tcggctgcgg tggacgggcg ggcgggcaag
     3781 ctgctcctgt tctcggccgt ggacaacctg ctcaagggag cttcgggggc ggcggtgcag
     3841 aacatgaacc tggcgctcgg gtacgaggag catctcgggc tcgagcatct cacctaggag
     3901 gacgttttgg gagctatggg aacacacgtg gacaagacga cgaggcagga tctcatcctg
     3961 cgcctcatct ccgagcgtga cctcggcacg cagcaggacg tggtcggggc gctggccgac
     4021 gagaacgtgg aggtcgccca ggccaccgtg agccgcgacc tcgccgagct aggggtactc
     4081 aaggttggca gccgttacct ggcgctgccg cacgaccccg gctccgccgg catcgaggtg
     4141 ctcccaagct tcgtcctcgg catcgtgcct gcccagaacc aggtcgtgat ccacacccgc
     4201 gacggcacgg cgggtgccgt ctccagcgtc ctcgacaggg tcaagggcct gaagatactc
     4261 ggcaccgtcg ccggccagga taccgtgctc gccgtagccc ccgacgctga cgctgcggcc
     4321 gaggttgcga acctcatcgc cgacgtgtgc cgcgccagga cccggcccgc tggcaacgtc
     4381 gaataatttg gggtcaggac gttcggaccg tgccggtgtt caggcccccg tgcccgccct
     4441 gacggtgctc gggggtcgcc ctttcatctc ccggtcctcc tcttacttcg ataccggcca
     4501 cttgctgccc ggggaagagc aactcgagct cgccgttggt aggctacggg ccggagatca
     4561 cagggtagga gat
//
LOCUS       DBNGVD010000581         1949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_340492, whole genome shotgun sequence.
ACCESSION   DBNGVD010000581 DBNGVD010000000
VERSION     DBNGVD010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_340492"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="AC16CM_15650"
     CDS             <1..143
                     /locus_tag="AC16CM_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HIR3141316.1"
                     /translation="PVIGLDVWEHAYYLKYQNRRPEYIQAWWDVLNWDEAERRFQAAK
                     NA"
     gene            389..1765
                     /locus_tag="AC16CM_15655"
     CDS             389..1765
                     /locus_tag="AC16CM_15655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3141317.1"
                     /translation="MKLVIVGGVAAGTKAASRARRLDPEMEITVYQEEPEPSISECGL
                     PYYLSGIVEERERLVARTPEQFAEKDIEVLVRHRVEGLDAGAKRLVVRDLHAGKTFED
                     TYDRLIVATGARAVLPPIPGSGLGGVFPLRFLTDADRIMSYMRERSPKKATIVGGGYI
                     GLEVAENLCGLGIECSVVEGENRVAVAYGPEVSERVADELRAHDVDIFTGERVEEFIG
                     EDRVRGVKFGDTELETDLVIVGVGIEPSVELAAEAGVEVGETGAIRVDEHMKTNVPDV
                     WAAGDCVETRDLTSGKQAWFPLGDTANQMGRVAGTNATTGEDTLEFPGTLGTGIFKVF
                     DLGVGRTGLSEEAAADAGFETVSASLTSYDRAAYYPGAGKVFLKLIADRSTGRLIGAE
                     AAGAFSDKLIDVCATALWGKLSYPDLVNIDLAYSPPYGPALSPVIQAATILSGKFERA
                     REGVRASE"
BASE COUNT          376 a          616 c          644 g          313 t
ORIGIN      
        1 cgccagttat agggttggac gtgtgggagc acgcctacta cctgaagtac cagaacaggc
       61 gtcctgagta catacaggcg tggtgggatg tgctgaactg ggacgaggcc gagaggcgct
      121 tccaggccgc caagaacgcc tagcccctag caaagaccag ccagcaggag ggcccccgga
      181 gcgttccggg ggccctcttc gcatctccga cgggtacccc gaagatcgca ccggcctggg
      241 ccgggtctct ggtgaccatc gaacccgtca tcacctgtga agcctctgtg atctctcgag
      301 cacgcatcat ccgtatacgt cgtggtccgg acagagcctg tagactctgt ccggcagtaa
      361 gggggcggac gtgggaagga tctcttagat gaagcttgtt atcgtgggtg gtgtggcggc
      421 ggggacgaag gcggcttcga gggcgcgcag gcttgacccc gagatggaga tcacggttta
      481 tcaggaggaa ccggaacctt cgatcagcga gtgcggcctg ccctactacc tctccggcat
      541 agtcgaggag cgtgaacggc tcgtggcacg caccccggag cagttcgcgg agaaagacat
      601 cgaggtgctg gtccgccacc gggtcgaggg cttagacgca ggcgcgaaaa ggctcgtcgt
      661 gcgcgatctc cacgcaggca agaccttcga agatacctac gatcggctca tcgtcgccac
      721 gggcgcgcgg gctgtcctcc cccccatccc cggctctggt ctcggaggcg ttttcccact
      781 tcgattcctc accgacgctg acaggatcat gtcttacatg cgcgagcgct cgccaaagaa
      841 ggccaccata gtgggcggag gttatatcgg gctcgaggtc gcggagaacc tttgcggcct
      901 cgggatagag tgctccgtgg tggaaggcga aaacagggtc gccgtcgcct acgggccaga
      961 agtctcagag agggtggccg acgaactgcg agcccacgac gtcgacatct ttacgggtga
     1021 gagggtcgag gagttcatcg gcgaagaccg cgtgcgcggc gtcaagttcg gcgacacgga
     1081 gctcgagacg gacctcgtga tcgttggcgt tggcatcgag ccaagcgtgg agctggccgc
     1141 tgaggcgggg gtggaggtcg gcgagacagg cgccatccgg gtagacgagc acatgaagac
     1201 caacgtcccc gacgtgtggg ccgccggtga ctgcgtggag accagggacc tgaccagcgg
     1261 caaacaggcc tggttccccc tcggggacac cgcgaaccag atgggccgcg tagccggcac
     1321 caacgccact acgggcgaag acaccctgga gtttccgggc acccttggca ccgggatctt
     1381 caaggtcttc gacctcgggg tgggccggac gggcctctca gaggaagcgg ccgcggacgc
     1441 cggcttcgag accgtgagcg cgagcctcac ctcctacgac cgcgccgcat actatcccgg
     1501 cgccggaaag gtcttcctca agctcatcgc cgaccgctcc acgggacgcc tgatcggcgc
     1561 cgaagcagcg ggcgctttct cggacaagct catcgacgtc tgcgccacgg ccctctgggg
     1621 aaagctctcc taccccgacc tggtcaacat agacctggcc tactccccac cctacggtcc
     1681 cgccctgagc cccgtcatac aggccgccac catactctcc ggcaaattcg aacgcgccag
     1741 ggaaggcgtc agagcctccg agtagtgcta gctatcagcc gtcagcgggc agcaaaaagc
     1801 tgatggctga tagctaaacg ctgatagctc tagaaccgct cgactaccgt cctcgcgagc
     1861 atgtgcagcg gcaggtagtc ggggccgccg gccttggagt cggtgccgct catgccgaag
     1921 ccgccgaagg gctgcacgcc caccagggc
//
LOCUS       DBNGVD010000582         3050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4714107, whole genome shotgun sequence.
ACCESSION   DBNGVD010000582 DBNGVD010000000
VERSION     DBNGVD010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4714107"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16CM_15660"
     CDS             complement(<1..559)
                     /locus_tag="AC16CM_15660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3141318.1"
                     /translation="MFGVLGTGTMGAGIVQLAAQSGYRVIACDASVESLERAQVYVRE
                     NLGRLAAKGTFPEDEAASHYDRIHWTTDPRDLAEAEAAIEAIVERVEPKKEALAMLDG
                     ILPDGALLLTNTSSISITDLASATDRPESVCGMHFFTPPPVREAVEIPRGRHTSDETV
                     GRVEALVRSLGKLPVVVDGDTPGFVA"
     gene            complement(565..1623)
                     /locus_tag="AC16CM_15665"
     CDS             complement(565..1623)
                     /locus_tag="AC16CM_15665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3141319.1"
                     /translation="MSETVDVRKGEDFDLEAVERYLREHIEDLPEGALEVSQFPSGAS
                     NLTYLLKIDGWEGVLRRPPLGPVPPKAHDMGRESGILSRLGAVYPLAPKPYFYCADES
                     VIGVPFYVMERRTGVVLDETFPDGVRPDGELCRGISRTVVDTLVRLHAVDVQEAGLGD
                     LGRPEGFLQRQTEAWISRYDKARTEDIGEVGPLTDWLSRGVPQSPPPTVIHNDYKLNN
                     LVLDPEDLTRVRAVLDWEMATIGDPLFDLAVSLTYWIEPDDPDELKAVMPTVTATPGF
                     MTRRDLVDRYEKGSGRDLSDMHWYLVFGYFKLAAILQQIFARWKNGQTEDERFADFGE
                     RVRTLILHADDLTRTGEV"
     gene            complement(1620..2366)
                     /locus_tag="AC16CM_15670"
     CDS             complement(1620..2366)
                     /locus_tag="AC16CM_15670"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR3141320.1"
                     /translation="MKRLRVLLTRQEIVPERLPGATVVVLDVFMATTTLLTILDNGAR
                     GIYPVASLEEAEEVAAGLRPENVLRGGEQDAARIEGYDHGPFPEEYGPEVVENRDVIF
                     VTTNGTRAVAEAAPADTVLLGTLRNAPAVARHLEGLETESIYLVCAGSAGRFALEDFL
                     GAATILSHMDVGGWHLNDGAWLAIDFAAGHAEDHSGLLKRSRAGRWFVENDRIDTLDF
                     VADLGASDLVPEVAAGRLRPATGADQGGAA"
     gene            2525..2965
                     /locus_tag="AC16CM_15675"
     CDS             2525..2965
                     /locus_tag="AC16CM_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141321.1"
                     /translation="MSEVERRKEEQPKRGGGHSFMYLTFGIGFVLAAIMVIITSGTAA
                     AGIGFVIPAAIFLGLAGNDFQKHRHTLPQPASKERELLSAIRDNGGSITPAEAAMETS
                     LTVREADGMLSELAGGGHLAVEGRDGTLFYTLPRRRDPEIEGGN"
BASE COUNT          500 a          981 c         1024 g          545 t
ORIGIN      
        1 tggccacgaa gccgggagta tcgccgtcaa cgacaaccgg gagcttgccg agagagcgga
       61 ccagggcctc gaccctccct acggtttcat cgctggtgtg gcgtccgcgc gggatctcga
      121 ctgcctcgcg taccggcggc ggggtgaaga aatgcatgcc gcagacgctc tcgggacggt
      181 ccgtggccga ggcgaggtcc gttatggaga tagaggaggt gttggtcagg aggagcgcgc
      241 cgtcggggag tatgccgtca agcatcgcca gcgcctcttt cttcggttcc accctctcga
      301 ctatggcctc tatagcggcc tctgcctcgg ccaggtctct cgggtccgtg gtccagtgga
      361 tgcggtcgta gtgggaggcc gcctcgtctt ccggaaaggt gccttttgcg gcgaggcgcc
      421 ccaggttttc gcggacgtag acctgggccc tctccaggga ctctacggag gcgtcgcagg
      481 cgatcacccg gtagccggac tgggctgcca gctgtacgat gccggcgccc atggttcccg
      541 tgcctaggac gccgaacatc cctatcagac ttcgccggtt cgggtcaggt cgtcagcgtg
      601 caggatcagg gttcgcacgc gctcgccgaa gtccgcgaag cgctcgtcct cggtctggcc
      661 gttcttccag cgggcgaaga tctgctgcag gatcgcggcc agcttgaagt atccgaagac
      721 caggtaccag tgcatgtccg agaggtcacg cccgctcccc ttctcgtagc ggtcgaccag
      781 gtcccggcgg gtcatgaagc cgggcgtggc cgtcacggtt ggcataacgg ccttgagctc
      841 gtccgggtcg tcgggttcga tccagtacgt gagcgagacg gcgaggtcga agagcgggtc
      901 cccgatggtg gccatctccc agtcgagtac ggcccttacc cgggtgagat cttccgggtc
      961 caggacgagg ttgtttagct tgtagtcgtt gtggataacg gtgggaggcg ggctctgggg
     1021 aacgccacgg gagagccagt cggtgagggg gccgacctcc cctatatctt ccgttctggc
     1081 cttgtcgtaa cgtgagatcc aggcttccgt ctgccgctgc aggaagccct cgggcctgcc
     1141 caggtccccg aggccggcct cctgcacgtc caccgcgtgc agcctgacga gcgtgtccac
     1201 gacggtgcga gagatgcccc ggcacaactc cccgtcgggc cgaacgccgt cggggaaggt
     1261 ctcgtcgagc acgaccccgg tcctgcgctc catcacgtag aagggcacgc ctatgaccga
     1321 ctcgtctgcg cagtagaagt agggtttcgg ggcgagcggg tagacggcgc cgagcctcga
     1381 gaggatgccc gactcgcgcc ccatgtcgtg cgctttgggt ggtacggggc caagcggtgg
     1441 gcgcctcaac acgccctccc aaccgtctat cttcaacagg tacgtgaggt tcgaggctcc
     1501 ggaggggaac tggctgacct ccagcgcacc ctccggcagg tcttctatgt gctccctcag
     1561 gtaacgctcc acggcttcga ggtcgaaatc ctcgcccttg cgcacgtcaa ccgtctcgct
     1621 caagccgctc ctccctggtc cgccccagtt gcggggcgga gtctgcccgc ggcgacctcg
     1681 gggacgagat cgctcgcccc caggtcggcc acgaaatcta aggtgtctat acggtcgttc
     1741 tcgacgaacc atcgaccggc ccgactccgc tttagaaggc cggagtggtc ttcggcgtga
     1801 cccgcggcga agtcgatggc cagccaggcg ccatcgttca ggtgccagcc tcccacgtcc
     1861 atgtgcgaga ggatagtggc cgccccgaga aagtcctcga gggcgaagcg tcccgcggag
     1921 ccggcacaga cgaggtagat ggattccgtc tccagcccct ccagatggcg ggccacggcg
     1981 ggtgcgttgc gcagggttcc aagcagtacc gtgtcggccg gtgccgcctc ggcaacggcg
     2041 cgggtgccgt tggtggtcac gaagatcaca tccctgttct cgacgacctc gggcccgtac
     2101 tcttctggaa acgggccatg atcgtagccc tctatgcggg ccgcgtcctg ctcgccgccg
     2161 cgcaggacgt tctccgggcg caggcccgcc gcgacctcct cggcctcttc gaggctggcc
     2221 acggggtaga tgccccttgc accattgtcc aggatggtga gcagggtcgt agtcgccatg
     2281 aacacgtcaa gcacgactac ggtcgcgccg ggaagcctct ccggcacgat ctcctgccgc
     2341 gtcaagagca ccctgagcct cttcaagaaa cctctcatcc ctctcgccgg aaagcccgca
     2401 accattctac agaaaatgcg ttcgcggtta cccgaagcca cggaccacgt ttgcactggg
     2461 gatggcccgg tctagaataa cgccggacat ccgtacgtcg ggccgtgggc ggtcgggagg
     2521 cgtaatgagc gaggtcgaga gacgtaaaga agagcaaccg aagcggggcg ggggccactc
     2581 gttcatgtat ctgaccttcg gtatcgggtt cgtgttggcc gctatcatgg tcatcatcac
     2641 ctcgggaacg gccgccgcgg gcatcggctt cgttataccg gcggccatct ttctcgggct
     2701 cgcggggaac gatttccaga agcaccgcca tacgctgcca cagccggcca gcaaggaacg
     2761 cgagctcctc tcggccatcc gcgacaacgg cggcagcata acgcccgcgg aggctgcgat
     2821 ggagacctcg ctaaccgtgc gcgaggccga cgggatgctg tccgagctcg cggggggcgg
     2881 gcatcttgcg gtggaaggcc gggacgggac cctgttctac acgctgcccc gcaggcgtga
     2941 tcccgagatc gaaggcggaa attaggcgtt ggggcgattc gttttcgggc ggttggcgaa
     3001 ccgcccctac aggtttgtca tagggcgaga gaggtcggcg cggtcgaggc
//
LOCUS       DBNGVD010000583         2079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2890104, whole genome shotgun sequence.
ACCESSION   DBNGVD010000583 DBNGVD010000000
VERSION     DBNGVD010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2890104"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC16CM_15680"
     CDS             complement(<1..543)
                     /locus_tag="AC16CM_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3141322.1"
                     /translation="MLGGARITYLGHSTFRFVTAGDEQIIIDPFLRNNPHCPEELKQV
                     GELDTMLITHGHFDHFDDVEPLWGQTGARTLSNFEIMSYLQRQGVENAEPIMKGGSAE
                     VGGIKVTATNAFHSSSIALDDGSLIYGGEPLGYLVEFESGFKLYHAGDTALFGDMRLI
                     GELYNPDIAFLPIGDRLTMGP"
     gene            709..1131
                     /locus_tag="AC16CM_15685"
     CDS             709..1131
                     /locus_tag="AC16CM_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3141323.1"
                     /translation="MKSTLILAALAGVCVAVQTSLTGAAQRSLGPAVLVAVSGLTTGF
                     CALLVSFFVARPEFTGRAVAYAVASGVLGAVIVGSIAVAAGQAGVARALSLVIAAQLI
                     AGLVLDSFGIFGAGADFSIPRVLGVALIVAGGALVVSY"
     gene            1259..>2079
                     /locus_tag="AC16CM_15690"
     CDS             1259..>2079
                     /locus_tag="AC16CM_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR3141324.1"
                     /translation="MPIEDHGIIGDLHTAALVGTDGTIDWLCLPAFDSPSVFCSILDD
                     EKGGHFSLRPVEYTRSQQIYLPDTNVLLTRFFSRGGLAEILDFMPLHSKTGERHKLVR
                     NVRVIRGRMDLRMECRPAFDYARRSHSVSLAGSGAVFSSEGFCLGLATETNLEEWDGG
                     AAGARFSLAAGETATFVLARVDQGEGPGEVLSAAGFEDLLHRTMRYWRGWLAKSTYHG
                     RWREMVNRSALVLKLMVYDPTGALVASPTMGLPESIGGERNWDYRYTWLRDAALTL"
BASE COUNT          332 a          634 c          694 g          419 t
ORIGIN      
        1 gggacccatg gtgagcctgt cgccgattgg caggaacgcg atatcagggt tgtagagttc
       61 cccgatcagc cgcatgtcgc caaagagggc cgtgtcacct gcatggtaga gcttgaaccc
      121 gctctcgaac tcgaccaaat aaccaagcgg ctcgccaccg tagatcaggc tcccgtcatc
      181 gagcgcgatg gacgaagagt ggaaggcgtt ggtagcggtt accttgatac cgccaacctc
      241 cgcgctgcct cccttcatga tcggctccgc gttctcgacg ccctggcgtt gcaggtagga
      301 catgatctcg aagttggaga gcgtcctcgc acccgtctgc ccccacagcg gctccacgtc
      361 gtcgaagtgg tcgaagtggc cgtgtgttat gagcatggtg tcgagctcgc cgacctgctt
      421 gagttcttcg gggcaatgcg ggttgttcct gaggaacggg tctatgatga tctgctcgtc
      481 gccggcagtt acgaacctga acgtcgagtg gccgaggtag gtaattctgg ccccgccaag
      541 catctcttcc acgattatcc tccgtacgtt cgccgattgc tctgcaaaaa aacaacatta
      601 gcaggtttac ccaactcgta taccggagaa acggttgcct gctgcgtata atcctgcgga
      661 tgcccgatcg gggctcctcg acgtaatcac gaccctcagg gagtaccaat gaaatccacc
      721 ctcatcctcg cggcgctggc cggcgtgtgc gtggcggttc agacgagcct gacgggggcc
      781 gcccagcgga gcctgggtcc ggcagttctg gtcgcggtca gcggccttac gaccggcttc
      841 tgcgccctgc tagtctcgtt ctttgtggcc cgaccggagt ttacgggacg ggcggtcgcc
      901 tatgctgtgg cctctggcgt gctcggtgcg gtgatcgtcg gttccatagc ggtggctgcg
      961 ggccaggccg gcgtggcccg cgccctctcc ctcgtgatag ccgcgcaact gatcgctggc
     1021 ctcgtgctcg actctttcgg catcttcggc gcgggggcgg atttcagcat ccccagagta
     1081 ctgggggtcg ccctgatcgt ggctggcggc gcgctggtcg tcagctacta gatcacgcgc
     1141 cgtctcggcc gcgctctcgg gttcctggcc ctgcggggca gggtattggg gcgtgagatt
     1201 gggggcgggg ctaagattag gccagggtct acactgtctg gaggttggat tggggtactt
     1261 gcccatcgaa gaccacggga tcatcggtga tcttcacacg gcggcgctgg tcggcacgga
     1321 cgggaccatt gactggttgt gcctgccggc tttcgattca cccagcgtgt tctgctccat
     1381 tctggacgac gagaagggcg ggcacttctc gctccggccc gtcgagtaca cgcggagtca
     1441 gcagatctac ctgcccgata ccaacgttct tctgacccgc ttcttctcca gggggggcct
     1501 ggcggagatt ctggacttca tgcccctcca ctcgaagacc ggggagcggc acaagctggt
     1561 gcggaacgtc cgcgtcataa ggggaaggat ggaccttcgg atggagtgca ggcccgcctt
     1621 cgactacgca cgccggagcc actctgtctc cctggccggg tcgggggccg tcttctcctc
     1681 ggaggggttc tgcctcggtc tggccaccga gacgaacctg gaggaatggg acgggggggc
     1741 cgccggggcg aggttctcgc tcgccgcggg cgagacggcg acgttcgttc tggcccgcgt
     1801 agaccagggt gaggggcccg gggaggtact ctcggctgcc gggttcgagg accttctgca
     1861 ccgtacgatg cgctactggc ggggatggct cgccaaaagc acctaccacg ggcgctggcg
     1921 ggagatggtg aaccgctccg cgctcgtgct gaagcttatg gtctacgacc ccacaggggc
     1981 tctcgtggcc tcacccacga tgggtttgcc agagagcatc ggcggcgaga ggaactggga
     2041 ctaccgttac acctggctca gggacgcggc gctgacgct
//
LOCUS       DBNGVD010000584         6759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1980883, whole genome shotgun sequence.
ACCESSION   DBNGVD010000584 DBNGVD010000000
VERSION     DBNGVD010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1980883"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="AC16CM_15695"
     CDS             complement(<1..84)
                     /locus_tag="AC16CM_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR3141325.1"
                     /translation="MDNHDKIRVRYAPSPTGRLHVGGVRTAL"
     gene            292..555
                     /locus_tag="AC16CM_15700"
     CDS             292..555
                     /locus_tag="AC16CM_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141326.1"
                     /translation="MADIEVTPIGAREFEVVLEGEGAHRVTVPEELVAELDLPEDDLK
                     GVVRESFGFLLEREPASSIMSEFSLDVIPNYFPEYREELPKRL"
     gene            complement(552..851)
                     /locus_tag="AC16CM_15705"
     CDS             complement(552..851)
                     /locus_tag="AC16CM_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3141327.1"
                     /translation="MSASMERSSNGPPGEQEIEARFREEDLSPHTWGNGPGYEYDWHT
                     HGYHKVLYCVSGSIVFHTDEGDFDLEPGDRLDVEAGTGHSATVGENGVRCMEAAR"
     gene            complement(1034..1987)
                     /locus_tag="AC16CM_15710"
     CDS             complement(1034..1987)
                     /locus_tag="AC16CM_15710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3141328.1"
                     /translation="MTKFGYFLSCEEFGPNELIEQAKMAEAAGFEALWISDHFHPWND
                     EQGESPFVWSVIGGLAQATSLPVTTGVTCPTVRIHPAIIAQAAATSKVMLNGRFSLGI
                     GSGEALNEHIFGDAWPSADVRLEMLEEAVEVMRLLWQGGTRDHEGKHYVVENARLYTL
                     PEEPPEVLISGFGPKAIELAARIGDGYCGVEPDPETVDLYRSSGGGNKPAHAGTKVCW
                     GEDEDAARKTALRLWPNDALPGELAQVLPTPRHFEQATQLVTEDMIGESFTCGPDPEK
                     HLQQLRAYADAGYDEVYVQQIGSEHEGFFRFYENEILPQLR"
     gene            complement(2161..2844)
                     /locus_tag="AC16CM_15715"
     CDS             complement(2161..2844)
                     /locus_tag="AC16CM_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3141329.1"
                     /translation="MESEQGDGLVATARETILWRINHATRDVPEGERPGDVPVERGYR
                     TEEDTDREELVARFAERTAEYDAKVSRVANEELSGAIEEALGRRGVKRLVVPPYLPKD
                     WMPEGVQTMPDSSRSRLTNQELDESDGVLTGCAYGIAQTGTIVLDAGRSQGRRALTLL
                     PDYHLCVVREEQIVGLVPEAFTKLEETVKAEGRAVTFISGPSATSDIELNRVEGVHGP
                     RTLEVLICG"
     gene            complement(2817..4277)
                     /locus_tag="AC16CM_15720"
     CDS             complement(2817..4277)
                     /locus_tag="AC16CM_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3141330.1"
                     /translation="MTTGLSDAFSPGPTPTFEQSARESLSDSQLRRNLGKATQTIRRK
                     RAVAVEEMPDWENLREAGRALKERVLRHLDEYLLELEESVTRAGGKVHWARDADEANE
                     IIGRIVKDHDSEEVVKVKSIATDETKLNEYLEREGIHAFETDLAELIIQLAGETSSHI
                     LVPAIHKNRAEIQELFSRELGAGDLSDEPSDLTEAARLYLREKFLKAKVGISGANFAV
                     AETGTVCVVESEGNGRMCTTLPPVLVTLMGIEKVIPAWRDFEVFMQLLPRSSTAERMN
                     PYTSFWTGVSEDDGPREFHLVLMDNGRTDVLADEIGRQTLNCIRCSACLNVCPVYERT
                     GGHAYNSVYPGPIGAILTPQLVGLGDPGPDSLPFASSLCGACYQVCPVKINIPRVLVH
                     LRGEVVRHKQDRGGPREMLDPENIAMQTVARVFSDQKLYERAQKFARIGQWPLVRGGS
                     IGRLPGRLAGWTSVRDLDPVPEQSFREWWKANRGTG"
     gene            complement(4274..5014)
                     /locus_tag="AC16CM_15725"
     CDS             complement(4274..5014)
                     /locus_tag="AC16CM_15725"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3141331.1"
                     /translation="MRAALFITCFNDTLFPGTGRATVEVLERLGVEVDFPLAQTCCGQ
                     MHFNTGYQREAIPLVRRFVEIFDDYDAVVAPSGSCVSMVRELYPMAAELAGDQDLAAR
                     IEDLGPKVFELSEFLAKKLGVTEVGAYYPHRVTYHPTCHSLRMLDVGDAPLSLLRNVR
                     GIDLVELSEAEECCGFGGTFAVKNADTSAAMLSDKIRHVLDTEAEVCTAGDNSCLMHI
                     GGGLGRLRAGVRTVHLAEILASQGGEGR"
     gene            5169..5474
                     /locus_tag="AC16CM_15730"
     CDS             5169..5474
                     /locus_tag="AC16CM_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HIR3141332.1"
                     /translation="MTDRITNPWGERKPFPRDGEWPARKDQYLQEGVSENVTAVRIGA
                     GALSVALTGAILLLLGSSHPPSGATTLMVGVVLLTAVGLLLNRAAGEPVPLWAAGKS"
     gene            5580..5954
                     /locus_tag="AC16CM_15735"
     CDS             5580..5954
                     /locus_tag="AC16CM_15735"
                     /inference="COORDINATES: protein motif:HMM:NF019401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIR3141333.1"
                     /translation="MEEYLVTAVGYLRLVVEAIGAAVVGFGVLATTYRYVLTLLGLRN
                     YTYNQIRLYLGRYLVLGLEFQLGADILSTAVAPTLDDVILLGAIATIRTVLNYFLSQE
                     LERERREEATLQDPAQERGVPG"
     gene            5965..6282
                     /locus_tag="AC16CM_15740"
     CDS             5965..6282
                     /locus_tag="AC16CM_15740"
                     /inference="COORDINATES: protein motif:HMM:NF019401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIR3141334.1"
                     /translation="MGVYYAAVDFLRMFALGVGSFVIFVGIARGVWLARGVGGRARVA
                     RQIGEHAALGLDLFIGATLLNLAINPTWTAIATTAITVVVRKLLTYSLNLQRREAGPS
                     TGS"
     gene            complement(6269..>6759)
                     /locus_tag="AC16CM_15745"
     CDS             complement(6269..>6759)
                     /locus_tag="AC16CM_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3141335.1"
                     /translation="GPRRALRIYGESLNQLKWAILTIASILGLAYLLNYSGMTYTLGL
                     TLAATGVLFPFFSAFIGWLGVALTGSDTSSNALFANLQKVTAQQIGVSPNLTVGANSS
                     GGVLGKMISPQNLAVGTSATGLQGREGDLLRLVLKWSIGLTVVMAILVTLQAYVLPFM
                     IP"
BASE COUNT         1081 a         2197 c         2212 g         1269 t
ORIGIN      
        1 gagcgccgtg cgtacgccgc cgacgtgcag cctccccgtc gggctcggcg cgtacctgac
       61 cctgatctta tcgtggttat ccatggggta aacctaacac accacccctt gcgagaacag
      121 cttgggatgg ctgttaacta tcagctatca gccttcagtt atcagcggtt agctttccgg
      181 gctgatcgcc gaaagacgag gatgtccttc gtggtgaagg tgcggttggt ttcggcccga
      241 gggggttgct gtgttaagct gcctcgttca cgctcggaaa ggttgtaagt tttggctgac
      301 atcgaggtaa caccgatcgg tgcccgtgag ttcgaggtcg tgctcgaagg ggagggtgcc
      361 caccgcgtca cggtgcctga ggagctggtg gcggagctgg accttcccga ggatgacctg
      421 aaaggcgtcg tacgggaatc tttcgggttt cttctggaga gggaaccggc ctcttcgatc
      481 atgtccgagt tctcgctgga cgtgatcccc aactacttcc ccgagtacag ggaggaactg
      541 ccgaagcggc tctagcgggc ggcctccata cagcggacgc cgttctcgcc taccgtggcg
      601 gaatgcccgg tgccggcttc cacgtccagc ctgtcacctg gttctagatc aaagtctccc
      661 tcgtccgtgt ggaatacgat gctgcccgag acgcagtaga gcactttgtg gtagccatgc
      721 gtgtgccagt cgtactcgta accaggtccg tttccccagg tgtgggggct caggtcttcc
      781 tccctgaacc tggcttcgat ctcctgctcg cccggcggcc cgttggagga tctctccatg
      841 ctggcagaca tgctggtctc cttcattcga gaatactggc ccatcctagc atggcgttct
      901 cgtgtgatgg acccgagatg cggatctcgc tggcgtgcca tcccggccag agcggtcgac
      961 gaccggccgg taaagacagg ccgatcgccg aaggcccccg aatctacgcc ggcgcgaccc
     1021 acaccagaag ggatcagcga agctggggca ggatctcgtt ctcgtagaaa cggaagaagc
     1081 cctcgtgctc ggatcctatc tgctgcacgt agacctcatc gtagccggcg tcggcgtacg
     1141 cccggagctg ctgcaggtgc ttctccgggt cggggccgca cgtgaaagac tcgcctatca
     1201 tgtcttcggt taccagctgg gtggcctgct cgaagtgccg cggggtgggc agcacctggg
     1261 cgagctcccc gggtagcgcg tcgttgggcc acagccgtag ggcggtcttg cgggcggcat
     1321 cttcgtcctc gccccagcac accttcgtgc cggcgtgggc gggtttgttg ccgccccctg
     1381 aagagcggta cagatccacc gtctcagggt cgggttccac gccgcagtag ccgtctccga
     1441 tcctggcggc gagctctatg gccttggggc cgaaaccaga gatcagaacc tccggcggct
     1501 cctccggcaa cgtgtacagc cgcgcgttct ccaccacgta gtgcttgcct tcgtggtctc
     1561 tggtgccgcc ctgccacagg aggcgcatga cctccacggc ctcctcgagc atctcgaggc
     1621 gtacgtctgc agacggccag gcgtcgccga agatgtgctc gtttagggcc tctccggagc
     1681 cgatgcccag cgagaaccgg ccgttcagca tcaccttgga ggtggcggcg gcctgggcga
     1741 tgatcgccgg gtggatgcga acggtggggc acgtgacgcc ggtggtcacg ggcagcgacg
     1801 tagcctgcgc caggcccccg atcacgctcc acacgaacgg agactctccc tgctcgtcgt
     1861 tccacgggtg gaagtggtcg ctgatccaca acgcttcgaa gccggcggcc tcggccatct
     1921 tggcctgctc gatgagctcg ttgggcccga actcctcgca ggacaggaaa tacccgaact
     1981 tcgtcaaggt ctcctcctat gctcgatggt gaatctcttg cctcgtcccg tccgtacccg
     2041 ggccagaccc gccggtaacg gccagcccgc cggcgcggac ggaaaatggc ccgaaaggtg
     2101 caggccgggt gtgatcccgg ggaatccggc tctaatggtg gatggtcagg ccgcgctgcg
     2161 tcagccgcag atgaggacct cgagggtacg cgggccgtgc acgccctcga ctcggttcag
     2221 ctctatgtcc gaggtcgccg aggggcccga gatgaaggta acggccctac cctcggcctt
     2281 taccgtctcc tcgagcttcg tgaacgcctc gggcacgagg ccgactatct gctcctcgcg
     2341 cacgacgcag agatggtagt cgggcaggag cgttagcgcc cggcgtcctt gcgaccttcc
     2401 ggcgtcgagc acgatggtgc cggtctgggc tatgccgtaa gcgcagccgg tcagcacgcc
     2461 gtcgctctcg tccagctcct ggttagtcag gcgggaacgg gacgagtctg gcatggtctg
     2521 tacgccttca ggcatccaat ccttgggcag gtagggtgga acgacgaggc gcttcacccc
     2581 gcgccgcccg agcgcttctt ctatggcccc ggagagctcc tcgttggcca cgcggctcac
     2641 cttcgcgtcg tactcggccg tgcgctccgc gaagcgcgcg accaactcct cccgatccgt
     2701 gtcctcttcc gtacggtacc cgcgctcgac cggcacgtcc ccgggccgtt ccccctcggg
     2761 cacgtcgcgg gtggcgtggt tgatacgcca caggatcgtc tctctggccg tcgccactag
     2821 cccgtccccc tgttcgcttt ccaccactcg cggaagctct gttcgggcac gggatccagg
     2881 tccctcacgg aggtccagcc ggccagcctg cccggcaggc ggcctatgga gcccccgcgt
     2941 accaggggcc actggccgat cctcgcgaac ttctgggccc tctcgtagag cttctggtcg
     3001 gagaacaccc tggcgaccgt ctgcatggcg atgttctccg ggtcgagcat ctcgcgcggg
     3061 ccacccctgt cctgcttgtg gcggaccacc tctccccgca ggtggaccag aacccggggg
     3121 atgttgatct tgacggggca gacctggtag cacgcgccgc agagcgatga cgcgaatggc
     3181 agcgagtcgg ggccggggtc gccgaggccg accagctggg gcgtgaggat ggcgccgata
     3241 ggaccggggt agacggagtt gtaggcgtgg ccgccggtcc gctcgtagac ggggcagacg
     3301 ttcaggcacg cggagcagcg gatgcagtta agcgtctggc gtccgatctc gtcggccagc
     3361 acgtcggtac gcccgttgtc catcagcacg aggtggaact cccgggggcc atcgtcctcc
     3421 gacaccccgg tccagaacga ggtgtagggg ttcatcctct ccgccgtcga ggagcggggg
     3481 aggagctgca tgaagacctc gaagtcccgc caggcgggta ttaccttctc tatgcccatc
     3541 agcgtcacga ggacgggagg cagcgtcgtg cacatcctgc cgttaccctc cgactcgacc
     3601 acgcagacgg ttcccgtctc ggccacggcg aagttcgccc cgcttatgcc gaccttcgcc
     3661 ttcaagaact tctcgcgcag gtacaggcgg gccgcctccg tcaggtcgga aggctcgtcg
     3721 gagaggtcgc ccgcgccgag ctctctggag aagagctcct gtatctccgc gcggttcttg
     3781 tggatggccg gcaccaggat gtgagagctg gtctcgccgg cgagctggat gatgagctcg
     3841 gcgaggtccg tctcgaaggc gtggatgcct tcccgttcca ggtactcgtt gagcttggtc
     3901 tcgtcggtcg ctattgactt gaccttgacg acctcttcgg agtcgtggtc cttgacgatg
     3961 cgaccgatga tctcgttagc ctcgtccgca tcgcgtgccc agtggacctt gccccctgcc
     4021 ctggtcactg attcttccaa ctcgagcagg tactcgtcca ggtggcgcaa gacgcgctct
     4081 ttgagggcgc gtcccgcctc cctgaggttc tcccagtcgg gcatctcctc gacggcgacg
     4141 gcgcgcttgc ggcggatggt ctgggtcgcc ttgccgaggt tgcgcctgag ctgcgagtcg
     4201 gagaggctct ccctggctga ctgctcgaac gtcggcgtcg ggcccggaga gaacgcgtcg
     4261 gaaagccctg tggtcatcgt ccctccccac cctggctggc gagtatctcg gcgaggtgca
     4321 cggtcctgac cccggcgcgg agccggccaa gcccgccgcc aatgtgcatg aggcaggagt
     4381 tgtccccggc cgtgcagacc tcggcctccg tgtcgagcac gtgcctgatc ttgtccgata
     4441 gcatggccgc cgaggtgtcc gcgttcttga ccgcgaaggt cccgccgaac ccgcaacact
     4501 cctcagcctc ggagagctcc accaggtcga tgccccgcac gttccgcagg aggctcagcg
     4561 gcgcatcacc tacgtcgagc atccgcaggg agtggcacgt ggggtggtag gtgacccggt
     4621 gcgggtagta ggctccgacc tcggtgacgc cgagcttctt tgccagaaac tcggagagct
     4681 cgaacacctt cgggccgagg tcctcgatcc tcgcggcgag atcctggtcc ccggccagct
     4741 cggcggccat cgggtagagc tcgcgcacca tgctcacgca ggagccggag ggcgctacca
     4801 cggcgtcgta atcgtcgaag atctccacga agcgcctgac cagcggtatc gcttcccgct
     4861 ggtatcccgt gttgaagtgc atctggccgc agcatgtctg cgccagaggg aaatcaacct
     4921 caaccccaag gcgttccagg acctcgacgg tggcccgtcc cgtcccgggg aagagcgtat
     4981 cgttgaaaca ggttatgaag agcgccgcgc gcaccgttgt gttcctccct ctactctagg
     5041 aaccccatga tagaccatcc cggccccctg gcgcggtcgc ggcgcccgtg gggcgtattc
     5101 gcgattacgg ccattagctc tggggagcac gaaaactatc aagcgttgtc gaggtgagga
     5161 gtatcaccgt gaccgaccgc ataacgaacc cctgggggga gagaaagcct ttcccccggg
     5221 acggggagtg gcccgcgagg aaagaccagt atcttcagga gggcgtctcc gagaacgtga
     5281 ccgccgtccg catcggggct ggcgccctgt ccgtggcgct cacgggcgcg atcctgctgc
     5341 tgcttggttc gtcacacccg ccgagcgggg cgaccaccct gatggttggc gtggtcctgc
     5401 tcaccgctgt cggcctgctc ctcaatcgtg ccgccgggga gccggtgccg ttgtgggctg
     5461 ccggaaaaag ttagacgacc cgcaaaaaac gggccatcac aatgttaggg ttttgcggtg
     5521 cgccggacgt cacgcgcagt ggttgcccaa cgttttgaga ccgaggagac gggatcgcga
     5581 tggaagaata cctcgtaacg gcggttggtt acctgcgcct ggtcgtcgag gccataggtg
     5641 ccgccgtggt tggtttcggc gtgctggcca cgacctatcg ttacgttctt accctgctcg
     5701 gcctcaggaa ttacacctac aaccagataa ggctctacct ggggcgctac ctcgtcctgg
     5761 gcctggagtt ccagctcggc gccgacatcc tctccacggc cgtcgcgccc acgctggacg
     5821 atgtgatcct gctcggcgcg atagcgacga tcaggacggt cctcaactac tttctctccc
     5881 aggagctgga gcgcgagcgt cgggaggagg ccaccttgca ggaccccgcc caggagcgcg
     5941 gagttcccgg ataggcaacc ccgaatgggc gtctactacg cggcggtcga cttcttgcgg
     6001 atgttcgcgc tcggcgtggg cagcttcgtg atctttgtcg ggatcgcacg cggcgtgtgg
     6061 ttggcccgcg gggtcggggg tagggccagg gtggcgcgtc agatcgggga gcacgccgcc
     6121 ctcggcctcg acctcttcat cggggccacc ctgctgaacc tcgctatcaa ccccacgtgg
     6181 acggccatcg cgacgaccgc catcaccgtc gtagtgcgca agctcttgac gtactcgttg
     6241 aatctgcagc ggcgggaggc cggtccgtct acgggatcat gaaggggagc acgtaggcct
     6301 ggagggtcac cagtattgcc attaccaccg tgagcccgat ggaccacttc aggaccaggc
     6361 gcaggaggtc gccctccctg ccctgcaggc ccgtggcgct cgtgcccacg gcgaggttct
     6421 gcggcgagat catcttgccc agaacgccgc cggaggagtt ggcgcccaca gtcaggttcg
     6481 ggcttacccc gatctgctgg gccgtgacct tctgcaggtt ggcgaagagg gcgttcgatg
     6541 acgtgtccga gcccgtgagc gccaccccga gccacccgat aaacgccgag aagaacggga
     6601 acaggacgcc cgtggccgcc agcgtcaacc ccagggtgta ggtcataccc gagtagttga
     6661 gcaggtaggc gaggccgagg atcgaagcta tcgtcaggat cgcccatttg agctggttga
     6721 gggattcgcc gtagatacgg agcgcccttc tggggccta
//
LOCUS       DBNGVD010000585         3866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3619891, whole genome shotgun sequence.
ACCESSION   DBNGVD010000585 DBNGVD010000000
VERSION     DBNGVD010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3619891"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="AC16CM_15750"
     CDS             <1..268
                     /locus_tag="AC16CM_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3141336.1"
                     /translation="PKDRRFDVSIEDGEMSPGEISVDRGDTVTLRVVADEPAELHVYG
                     YHVEQQVRPGKMAEIGFEADLTGRFEIEDHETEKELGVLQVRPR"
     gene            279..1775
                     /locus_tag="AC16CM_15755"
     CDS             279..1775
                     /locus_tag="AC16CM_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141337.1"
                     /translation="MRRLATTAVALSPLLVCASPAMAHGFGPTYDIPIPLWLYLFGAA
                     AAVVLAFVPLALSTRKIPVADDVYEYPRFDLLGIQWLGNVLGSRLLLGSLRALSVFIF
                     LVVVVSGLAGVQSGFNMAPTFVWITWWVGFGYFTALVGNLWPLVNPWGVLFDWAECLA
                     RRLGCRDGPGEAHPTALGIWPATGLYLVFVWFENVFSGSYMPRNIAFFALGYSLITLY
                     AMSYFGKETWFRHGEAFSVFFDLLGKFAPTEVRVKTPTVCLDCDSCDPGGCVNCYACF
                     RHADPQERELNIRPFAVGLRLPEKALPGVAAFVILVLAGVTFDGLLDTPLWLEVVRVT
                     PVDQTLGVMLLPMMFFVIYLGFVRLSLAFGGGEGASFGWFAAAYAFSLVPIAIAYQMA
                     HYYTYLLIQGQIIFSLVSDPLGRGWNLFGTAGFDPRYGIIGASFVWYSQVALIVVGHV
                     IAVYLAHSISLRLLSDPGRALRSQLPMLVLMVLYTITSLWILAQPIVE"
     gene            complement(1840..3252)
                     /locus_tag="AC16CM_15760"
     CDS             complement(1840..3252)
                     /locus_tag="AC16CM_15760"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3141338.1"
                     /translation="MQVATFVAYLAAAALFIYGIRRLRTPETARSGNTLAAAGMVVAL
                     IATVFVADIDDVLSVGQISIGVLAGGVVGAVAAQRVQMTAMPQMVAAFNGVGGGAAAL
                     IAVSEFYRVEALGQGEETIVAAIAVLLSVVIGTVSFAGSIIAFTKLQELALTGSVTFP
                     LQQVVNAAIFLVIVLLSVNIVAGGPVLSPLVSLYLILAASLLLGVLFVIPIGGADMPI
                     VISLLNAFTGLAAAASGFVLSNYALIISGTLVGASGTILTRLMSGALGRPLGKIIFAG
                     IAASGTRSGGQQEDREVNDASASEVGEYLADEAEKVIIVPGYGLAVAQAQQAMRDLVQ
                     TLESEGKEVLFGIHPVAGRMPGHMNVLLTEVGIPYDKLYDIEDINPEFDDADAAIVVG
                     ANDVVNPSANDPEVESPISGMPVLTVENAEHVIFIKRSLSPGFAGVDNPLFYDTEKTM
                     MLFSDAKQGLQDLNEAVKNS"
     gene            complement(3256..3564)
                     /locus_tag="AC16CM_15765"
     CDS             complement(3256..3564)
                     /locus_tag="AC16CM_15765"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR3141339.1"
                     /translation="MTSQLLAELAIFVIAAFLGFELISRVPNLLHTPLMSATNAIHGI
                     VLVGAIIALASASDLFTSTVGMIAVFFGAMNVVGGFWVTNRMLGMFGPPKKRVKKRGE
                     "
     gene            complement(3561..>3866)
                     /locus_tag="AC16CM_15770"
     CDS             complement(3561..>3866)
                     /locus_tag="AC16CM_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HIR3141340.1"
                     /translation="AAESGGNCELTEAGETVEHEGVRIVGPVNLPSTMPIHASQLYSR
                     NMYNLIGHITEDVAEDKDETDLRLDLDFEDEIISETCIAHDGEIRHERTREALQEAG"
BASE COUNT          642 a         1255 c         1221 g          748 t
ORIGIN      
        1 gccgaaggac cgacgtttcg acgtctctat cgaggatggc gagatgagcc cgggggagat
       61 ctccgtcgat cggggcgaca ccgttacgct ccgcgtcgtc gccgacgagc cagcggagct
      121 tcacgtgtac ggctaccacg tggagcagca ggttaggccg ggaaagatgg ccgagatcgg
      181 tttcgaggct gacctcaccg ggcgcttcga gatagaagac cacgagaccg agaaggagct
      241 cggcgtgctc caggtgcggc ctcggtaagg ggagcacgat gcgccgtctg gcaaccacag
      301 cggtggcgct ctcgccgctg ctggtatgcg cgagtccagc gatggcccac ggcttcgggc
      361 cgacctacga catcccgatc cccctctggc tctacctttt cggcgcggcg gcggccgtcg
      421 tgcttgcctt cgtcccgctg gccctctcca cccgcaagat tccggtcgcc gacgacgtct
      481 acgagtatcc ccgcttcgac ctgttaggca tccagtggct cgggaacgtg ttgggctcgc
      541 gcctcctcct gggatcattg cgcgcgctct ccgtgttcat atttctcgtc gtggtggtct
      601 ccgggctggc aggtgtgcag agcggcttca acatggcccc gaccttcgtg tggatcacct
      661 ggtgggtggg cttcggctac tttaccgcgc tcgtcggaaa cctgtggccg ctcgtgaacc
      721 cctggggcgt tttgttcgat tgggccgagt gcctcgcccg caggctgggt tgccgggacg
      781 ggccggggga ggcccaccca acagcgctcg gcatctggcc cgccacggga ctctacctcg
      841 tgttcgtgtg gttcgagaac gtcttctcgg ggtcctacat gccgcgcaac atcgccttct
      901 tcgcgctagg gtactccctg atcacgctct acgccatgtc ctacttcggc aaggagacgt
      961 ggtttcgtca cggtgaggcg ttctccgtgt tcttcgacct gctaggcaag ttcgcgccca
     1021 cggaagtaag ggtgaagacg ccaaccgtat gcctcgattg cgactcgtgc gatccgggag
     1081 ggtgcgtgaa ctgctacgcg tgcttccggc acgccgaccc tcaagagcgc gagctgaata
     1141 ttcgcccctt cgccgtcggc ctccggcttc cggagaaagc cttgcccggg gttgccgcgt
     1201 tcgtgattct ggtccttgct ggtgtaacgt tcgacggcct cctcgacacc ccgctctggc
     1261 tggaggtagt ccgtgtcacc cccgtcgacc agacgctggg cgtgatgctg ttgcccatga
     1321 tgtttttcgt catctacctc ggcttcgtga ggctcagcct ggccttcggt gggggagagg
     1381 gagcgagctt cgggtggttc gcggccgcct acgcgttctc cctcgtgccc atcgccatcg
     1441 cctaccagat ggcccactac tacacgtacc tgctcatcca ggggcagata atcttctccc
     1501 tcgtctccga cccgctcggg cggggatgga acctcttcgg caccgcgggc ttcgaccccc
     1561 gctacggcat aatcggggcg agcttcgtct ggtactcgca ggtcgccctg atcgtggtcg
     1621 gacacgtcat cgccgtctac ctggctcact ccatctcgct gcgcctcctt agcgaccccg
     1681 gacgcgccct caggagtcag ctcccgatgc tagtactgat ggtcctctac accataacga
     1741 gcctctggat actcgcacag cccatagtcg aatagagtaa acccgcgaaa gagagaagca
     1801 tcgggcctga ttgctgaaac ctgtagcctg atgccttctt cagctgttct tgacggcctc
     1861 gttcagatcc tgcaagccct gtttggcgtc ggagaagagc atcatggtct tctcggtgtc
     1921 gtagaagagc gggttgtcca cgccggcgaa gccggggctc agggagcgct tgataaagat
     1981 cacgtgctca gcgttttcga cggtcaggac gggcatcccg ctaatgggac tctctacctc
     2041 ggggtcgttg gccgacgggt tgaccacgtc gttggcaccg acgacgatcg ccgcgtccgc
     2101 gtcgtcgaac tcggggttga tgtcctcgat gtcgtagagc ttgtcgtagg ggatgccgac
     2161 ctcggtcagg agcacgttca tgtggcccgg catgcgcccc gccaccgggt gaatgccgaa
     2221 aaggacctcc ttgccctcgg attccagcgt ctgtacgagg tcgcgcatcg cttgctgggc
     2281 ctgggccacg gcgagaccgt agcccggcac tatgatgacc ttctccgcct cgtccgccag
     2341 gtactcgccg acctccgagg cactcgcgtc gttcacctcc cgatcttcct gctggccgcc
     2401 cgatctcgtt cccgaggcgg cgatgccggc gaagatgatc ttgccgaggg gacgccccag
     2461 ggcacccgac atgagccggg tcaggatggt gccagaggca ccaaccagcg tgccgctgat
     2521 gatcagggcg tagttggaga gcacgaagcc cgaggccgcc gcggccagcc cggtaaaggc
     2581 gttgagaagc gagatcacga tgggcatgtc cgccccgccg atggggatca cgaacagcac
     2641 gccgagcagc aacgaggcag cgaggatcag gtacagcgag acgagcggcg agagaacggg
     2701 gccgcccgcg acgatgttca ccgagaggag aacgatcacc aggaatatcg cggcgttgac
     2761 gacctgttgg agcgggaacg tcacggagcc ggtcagggcc agctcctgaa gtttggtaaa
     2821 cgcgatgatg cttcccgcga agctcaccgt cccgatcacg acgctcagga gcacggcgat
     2881 ggcggcgact atggtctcct cgccctgtcc cagcgcctcg acccggtaga actccgagac
     2941 ggcgatgagc gccgccgcgc cgcccccgac gccgttgaac gcagccacca tctggggcat
     3001 cgcggtcatc tgcacccgct gggccgcgac ggcccctacc actcccccgg ccagaacccc
     3061 gatgcttatc tgacctacgc taagcacatc gtctatgtcg gcgacgaaga cggtggcgat
     3121 gagcgccacg accataccgg ccgcggcgag cgtgttgccc gaacgcgccg tctccggcgt
     3181 tctaaggcgc ctgatgccgt agatgaacag cgcggccgcc gcgagatagg cgacgaacgt
     3241 tgcgacctgc aagggctatt ctcccctctt cttgacccgt ttcttggggg ggccgaacat
     3301 gccgagcatc cggttcgtga cccagaaacc gccgaccacg ttcatcgccc cgaagaagac
     3361 ggcgatcatg ccgaccgtcg aggtaaacag gtcggaggct gaggcgaggg cgatgatcgc
     3421 gccaaccagc acgatcccgt gtatggcgtt ggtcgcgctc atcaggggcg tgtgcagaag
     3481 gttggggaca cgggagatca gctcgaaccc gaggaacgcc gcgatgacga agatggccaa
     3541 ctctgccagt aattgcgagg tcatccggcc tcctgtagag cttctctggt cctttcgtga
     3601 cggatctcgc catcatgcgc gatgcaggtc tccgagatga tctcatcctc gaagtccagg
     3661 tcgagtcgaa ggtccgtctc gtccttgtcc tctgccacgt cttcggtgat gtgcccgatg
     3721 aggttgtaca tgttgcgcga gtagagctgg ctagcgtgta tgggcatggt gctcggcagg
     3781 tttacggggc ccacgatcct gaccccctcg tgctccacgg tctccccggc ctcggtgagc
     3841 tcgcagttgc ccccgctctc cgcggc
//
LOCUS       DBNGVD010000586         4804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_887604, whole genome shotgun sequence.
ACCESSION   DBNGVD010000586 DBNGVD010000000
VERSION     DBNGVD010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_887604"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..905
                     /gene="pdxS"
                     /locus_tag="AC16CM_15775"
     CDS             9..905
                     /gene="pdxS"
                     /locus_tag="AC16CM_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR3141341.1"
                     /translation="MAENGQSTGTFRVKSGMAQMLKGGVIMDVVDAEQARIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEKIVEIQDAVSIPVMAKARIGHFVEAQILEALEV
                     DYIDESEVLTPADESHHIDKAVFEVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRRLGVLEPEELMTEAKTLGAPYELVRWVAENGRLPVVLFTAGG
                     LATPADAALMMQLGADGVFVGSGIFKSGDPAVRARAIVKATTHYKDAKVIAEASRGLG
                     EAMVGREMGDLAEGERLATRGW"
     gene            895..1515
                     /gene="pdxT"
                     /locus_tag="AC16CM_15780"
     CDS             895..1515
                     /gene="pdxT"
                     /locus_tag="AC16CM_15780"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR3141342.1"
                     /translation="MAGKTETNGALPGEDSPARIGILALQGDFKEHAEILRGLGVEPV
                     EVRAVEDLEGLAGIIVPGGESTTIGKMMVSSGLLDGIRSYFYKGGPVWGTCAGMVLAA
                     SATTGPRQPLLGLMNALVERNGFGRQVHSFETDLQVEGFEEPFTGVFIRAPFFEDVGP
                     GVEVLSRVGERVVAARGENILVTAFHPELTDDVRFHEYFLREVCSI"
     gene            1512..2264
                     /locus_tag="AC16CM_15785"
     CDS             1512..2264
                     /locus_tag="AC16CM_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3141343.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSRAITVAAREGGGDTE
                     MNPALQLAVQKARDANMPNDNIQRAVDKGTGAGSDAETFERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYVFSKNGGKLGTSGSVAYLFERKGIILVPKDAADEDELMEIALES
                     GAEDVAEQENDYRIVTSPEDFRAVREALGVANVALENAEITMIPQNSIDLDAGTARQT
                     LRLIDALEENDDVQEVYANFDISEEVMAEVVN"
     gene            2389..2676
                     /locus_tag="AC16CM_15790"
     CDS             2389..2676
                     /locus_tag="AC16CM_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR3141344.1"
                     /translation="MVPTTRKEDDRNAIFMDHHKHIEGLTADAVADAQRKDEEIQEQY
                     GVNYHRYWFNEGTLEVFCLVEAPNKEAAVAVRREAHGLLADEITEVEEGQP"
     gene            3002..3928
                     /locus_tag="AC16CM_15795"
     CDS             3002..3928
                     /locus_tag="AC16CM_15795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3141345.1"
                     /translation="MVARLRGTLSLREMMIHPVRTGYLGQQDPIDPKLLPPSAEKLFP
                     NVAVTRAFAPSPDGPIRCEVFRPEGAPQNLPMMLYFHGGGFMVGRSEDTEFLTRKMCV
                     QNGLVVVSVNYRLAPEWPFPTGLDDCVAVYRWILEQGGTLGADTSRVVFAGDSSGSNF
                     AVTAALRARDADLPLPAAVVMLGPVCDFDFEQYDSFNRLAPKGIVYDAAFAGFMRGAY
                     VSHEDWPHPHVSPVHADLRGFPPTILVVGTADPMIDSARAFARRLEETGNRGVELFVS
                     ERMPHGFYFFPRLFREEEEAYEAVEKFLQKHL"
     gene            4301..>4804
                     /locus_tag="AC16CM_15800"
     CDS             4301..>4804
                     /locus_tag="AC16CM_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141346.1"
                     /translation="MALKVTGIDRRDNGGEADWMALSATQESVQTAMGYALEAQEMNT
                     RILRLTSQAWAESFRLQSTLFQGMARSFFGQWENALASATSVESLGQEQSRSKLTGPT
                     TDSAEDTEVAVDEAHQKTEKEKAEKQGADEVATEMRRAEAVAEELQKSTATATFPKEG
                     YDEMDVGE"
BASE COUNT          934 a         1434 c         1631 g          805 t
ORIGIN      
        1 agaattatat ggcggagaac gggcagtcga caggaacttt cagggtaaag agcggtatgg
       61 cgcagatgct caagggcggc gtcatcatgg acgtggtcga cgccgagcag gccaggatcg
      121 ccgaggaagc cggggccgtc gcggtcatgg cgctcgagcg cgtccccgct gacatcaggg
      181 ccgatggcgg ggtcgccagg atgagcgatc ccgagaagat cgtcgagatc caggacgccg
      241 tctccatccc ggtgatggcg aaggcacgca tcggccactt cgttgaggcc cagatcctgg
      301 aggccctcga ggtcgactac atcgacgagt cagaggttct cacgccggca gacgagagcc
      361 atcacataga caaggccgtg ttcgaggtgc ccttcgtgtg tggggccacc aatctggggg
      421 aggccctccg gcggatcggc gagggagcgg ccatgatccg gtccaagggc gaggccggca
      481 ccggcaacgt ggtcgaggcc gtgcgccaca tgcgcgccat cgttggcggg atccgtcgcc
      541 tcggcgtcct cgaaccggag gagctgatga cggaggccaa gaccctcggg gctccctacg
      601 agctggtgcg gtgggtcgcg gagaacggtc gccttcctgt cgtgttgttt acggctggcg
      661 gtctcgccac accggcagac gccgcgctca tgatgcagct cggcgccgac ggcgtgttcg
      721 tgggcagcgg catcttcaag agcggcgacc cggccgtgcg cgcccgggcc atcgtgaagg
      781 cgaccaccca ctacaaggac gcgaaggtca tagccgaggc gagcaggggg ctcggcgagg
      841 cgatggtcgg cagagagatg ggggaccttg cggaagggga gcggctcgcg acccgtggct
      901 ggtaagaccg agaccaacgg cgccttgccc ggagaggata gtccggcacg tatcgggatc
      961 ctcgcgctac agggcgactt caaggagcac gcggagatcc tgcgcggcct gggcgtcgag
     1021 cccgtggagg tcagggccgt ggaggacctg gagggcctgg ccgggatcat cgtccccggc
     1081 ggggagtcga ccacgatagg caagatgatg gtctcctcgg ggttgcttga cggcatacgc
     1141 agctacttct acaagggcgg accggtctgg ggcacttgcg cggggatggt gctcgcggcg
     1201 tcggcgacga ccgggccccg ccagccgctg ctcggcctga tgaacgcgct cgtagagcgc
     1261 aacgggttcg ggcggcaggt tcactccttc gagacggatc tccaggtcga gggcttcgaa
     1321 gagccgttca cgggcgtctt tatccgggca ccgttcttcg aggacgtggg gccaggggtc
     1381 gaagttcttt ccagggttgg ggagcgggtg gtcgcggccc ggggggagaa catcctggtc
     1441 acagcgttcc atcccgagct cacggacgac gtcaggttcc acgagtactt tttgcgggag
     1501 gtttgttcca tatgagcggt catagcaagt ggtcaacgat caagagaaag aaaggtgccg
     1561 ccgacgcgaa gcgcggggca ctgttcggca agttgtcgcg ggcgatcacg gtcgccgcgc
     1621 gtgagggcgg cggcgatacg gagatgaacc cggcgctgca gctcgcggtt cagaaggcca
     1681 gggacgccaa catgcccaac gacaacatcc agcgggccgt cgacaagggt acgggcgcgg
     1741 gatccgacgc cgagaccttc gagcgcataa cctacgaagg ctacgcgcct ggcggggtcg
     1801 ccgtcctcgt cgacgtgcta accgataacc gaaacagggc cgcctcagac gtccgctacg
     1861 tcttctccaa gaacggcggc aagctcggca caagcggctc cgtcgcctac ctcttcgaga
     1921 gaaagggcat tatcctggtg cccaaggacg ccgcggacga ggacgagctg atggagatcg
     1981 cactcgagtc aggagccgag gacgtcgccg agcaggagaa cgactaccgg atcgtcacgt
     2041 cgccggagga tttcagggct gtaagggagg cccttggcgt ggcgaacgtg gctctggaga
     2101 acgcggagat caccatgatc ccccagaaca gcatcgacct cgacgctgga accgcgaggc
     2161 agaccctgcg ccttatagat gcgctcgaag agaacgacga cgtgcaagag gtctatgcca
     2221 acttcgatat ctccgaagag gtcatggccg aggtagtgaa ctagcgaccg gacccgggcc
     2281 acgcccttcc gctccggtgg cccccttctg ccgcgttcct tgtctctcgg gccggttttg
     2341 gcgtgtggtt gccacacgtg tttccgtgcc agatagaggg tgccgaacat ggtgcccacg
     2401 acgcggaagg aggacgaccg gaatgccatt ttcatggacc accacaagca catcgaggga
     2461 ctgaccgcgg acgccgtggc cgacgcccaa aggaaggacg aggagataca ggagcagtac
     2521 ggggtcaact accatcgcta ctggttcaac gagggcaccc tagaggtctt ctgcctggtc
     2581 gaagctccca acaaagaggc ggcagtagca gttcgcaggg aggcccacgg gcttctggcc
     2641 gacgagatta ccgaggtcga ggaaggccag ccttagcgcg ttgtagtcga cgatgggggc
     2701 cggcagatat cttctcggcc tgacacggtt gccgacgact gcgagcatgt gcacatcgta
     2761 gtgcaccaat aatccgagca gcttgcagaa gaatcgaccc tcctcgaagg aaagccatca
     2821 gcggtcagcc ttcagccaga aaagctgata gctgaccgct cgctctccac caggtcagtc
     2881 attcagcaga attgctctgg gacggcccac cggggcggcc gcgagaagtg agcaggcatg
     2941 aggaggaacg tggccccttt ctcatatcga cccccacgat ccggcggcac cgaactacga
     3001 ggtggtcgcg cggctgcggg ggacgctgtc gctgcgcgag atgatgatcc atccggtgcg
     3061 cactggatac ctgggccagc aggatcccat cgacccgaag ctgttgcccc catccgccga
     3121 gaagttgttc ccgaacgtgg ccgtgacccg ggccttcgcg ccgtcgccag acggccctat
     3181 tcgttgcgag gtttttcggc cggagggggc gccccagaac ctgccgatga tgctgtactt
     3241 ccacggcggg ggctttatgg ttggccggtc ggaggacacg gagttcttga cccggaagat
     3301 gtgcgtgcaa aacggtctgg tcgtggttag cgtgaactac cgtctggcgc cggagtggcc
     3361 gttcccgacc ggcctcgacg attgcgttgc cgtataccgc tggatcctgg agcagggcgg
     3421 cacgctcggg gctgacacca gccgcgtcgt cttcgccggc gattcctccg ggtcgaactt
     3481 tgcagtgacc gcggcgctgc gagcacgcga tgcggacctg ccgctgcccg cggcggtggt
     3541 gatgctcggt cctgtatgcg atttcgactt cgaacaatac gactcgttca accggctcgc
     3601 ccccaaggga atcgtctacg acgccgcttt cgcggggttt atgcgcggag catacgtgag
     3661 ccatgaagac tggccccacc cccacgttag cccggtccac gccgacctgc gcggcttccc
     3721 gccaacgatc ctcgtcgtcg ggaccgccga ccccatgatc gatagcgccc gagctttcgc
     3781 ccgcaggctc gaagagacgg gcaacagggg ggtcgagctc ttcgtgagcg agaggatgcc
     3841 gcacggcttc tactttttcc cgaggctgtt ccgggaagag gaagaagcct acgaagcagt
     3901 cgagaagttc ctgcaaaagc acttgtaagt atagccccgt cgcgcgaggc ccaggttgcg
     3961 gcgggtgaac catcccacct agtcctcgtc caaccggcct cgaagcctgc ggacaccacc
     4021 cttgccttag gtcgcttcgg gtatgagttt gtggcggcgg tcccggcggc tgcatcatcc
     4081 ctactcaacc gcttcgcata ccgttgtagt tccggtaaac ccgcaacttt tatctctggc
     4141 acgccatctc atcccgagcc cggcggttcc tgaccgctaa ccgtggtcct ccgtatgggc
     4201 ttacttcgaa agcacgccgt gacaacgtgt ttgatcttct tgtgtgcagg gtacatatat
     4261 attcataaac catttactca catgaaacgg aggcgcgtct atggcgctga aagtcactgg
     4321 tatcgacagg agggataacg ggggagaagc cgactggatg gcgttgtcgg ccacccagga
     4381 gtctgtacag accgcgatgg gttacgcact cgaggctcag gagatgaaca cccggatcct
     4441 gcgtctgacg tcgcaggctt gggccgagag cttccgcctc cagtcaacgc tcttccaggg
     4501 catggcccgg tcgtttttcg ggcagtggga gaacgcgttg gcgagcgcga cgtctgtgga
     4561 gagcctgggc caggagcaga gcaggtccaa actcacgggg cccaccaccg acagcgcgga
     4621 ggacaccgag gtagccgttg acgaagccca ccagaaaacc gagaaggaga aagccgagaa
     4681 gcagggggca gatgaggtag ccacagagat gcggagggcc gaggcggtcg ccgaggaatt
     4741 gcagaagagt actgcgacgg ccaccttccc gaaagagggt tacgacgaga tggatgtggg
     4801 cgag
//
LOCUS       DBNGVD010000587         3770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3985239, whole genome shotgun sequence.
ACCESSION   DBNGVD010000587 DBNGVD010000000
VERSION     DBNGVD010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3985239"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..692)
                     /locus_tag="AC16CM_15805"
     CDS             complement(12..692)
                     /locus_tag="AC16CM_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141347.1"
                     /translation="MVETNTIRGVWLGGLSIVWRYPLATLVPSLVLGAIGEVPSYLID
                     ERRVVDLIVSLVTGYLAYYLYLAYAEGIVRKVRRGEQSRGLRSFFEDLAGAVPYVPGV
                     VGAALIVVAITSAATLLLVVPGVWLYTRWSLTTPVIREEGVGPVAAMRRSRELVREHF
                     WFVFMTASVAYYLEGILVDAGAWGLGTITGSLTWGEWVGGSILAALVMPVAAFTTSLA
                     HSNLEKRS"
     gene            complement(901..1146)
                     /locus_tag="AC16CM_15810"
     CDS             complement(901..1146)
                     /locus_tag="AC16CM_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141348.1"
                     /translation="MIASPETAVEEIAAFGPHMLLHNDNDEIGSEAVADIHYPITVHY
                     TDGMDARINMRGHIREAQDMSSDDLLRILDSVGDITG"
     gene            complement(1689..3080)
                     /gene="ggh"
                     /locus_tag="AC16CM_15815"
     CDS             complement(1689..3080)
                     /gene="ggh"
                     /locus_tag="AC16CM_15815"
                     /EC_number="3.2.1.208"
                     /inference="COORDINATES: protein motif:HMM:NF047898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004573 - Glc3Man9GlcNAc2 oligosaccharide
                     glucosidase activity [Evidence IEA];
                     GO_function: GO:0102547 - glucosylglycerate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate hydrolase"
                     /protein_id="HIR3141349.1"
                     /translation="MREEDLEGSLSKAAASRRFDGVNPEATSVLRRNGLKSWTKPAPS
                     LYPHQWSWDSAFIALGLAHVDNRRATDELDSLFRGQWSTGKVPHIVFDPEAPPRSYFP
                     DAERWNSSALSQDAPEGKHTSGLCQPPVHAIAVRRIWQTSRGKDEERATRARNFLRSN
                     YRRLFSWHRYLATARDPEGSGLVTIYHPWESGTDNSPRWDAALERISVGEVPSYTRYD
                     LQHVADPSHRPTDSEYDRFLWLLELLKRARYDEADIYERHPFLIKDVLFSAILVAANE
                     ALLEISEVVDAPEEDKELIGAWIARGRAGLEEQWDPGLGLCLDHDVLAGESVPVRTIA
                     GFSPLIAGGLDRRRLDTLLATLESEHFAGNADLRYPLPPSTSPVEPGFHPRSYWRGPV
                     WPVANWLLWWSLLRAGESTRAQNIRRDTLEELSVGGFAEYFEPFTGEPLGSGEQSWTA
                     AIALDMLAIGGAD"
     gene            complement(3169..3504)
                     /locus_tag="AC16CM_15820"
     CDS             complement(3169..3504)
                     /locus_tag="AC16CM_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141350.1"
                     /translation="MDEREARRNREVVERLDEIAREGYKSAVDQAFEAQKSGMKLSRR
                     FFENWVETLDDHAELHRRTLSNIQELVREQREVFHELSRESGEAYDGFLESLDAYQDE
                     IIGSDRRKD"
BASE COUNT          702 a         1193 c         1192 g          683 t
ORIGIN      
        1 gaaggcccga ttcaagaacg cttctcgagg ttggagtggg ccagcgaggt ggtgaaggcc
       61 gccacgggca tgaccagcgc ggccagtatc gagccgccaa cccactcccc ccaggtgagt
      121 gatcccgtga tcgtccctag cccccaggcg cccgcatcta ctagaatccc ttccaggtag
      181 taggccacag acgcggtcat gaacacgaac cagaaatgtt cccggaccag ttccctgctg
      241 cgtcgcatcg cggccacggg ccccacgccc tcttcccgga tcacgggcgt cgtcagcgac
      301 cagcgcgtgt acagccagac cccgggaacg acgagcagga gcgtcgcagc agaggtgatg
      361 gcaaccacga tcagggcggc tccgaccaca ccgggaacgt aaggtacggc cccggccaga
      421 tcttcgaaga agctcctgag gccacggctc tgctctcccc gccggacctt cctgacgatg
      481 ccttcggcgt aagcaaggta gaggtagtag gcgaggtagc ccgtcaccag cgaaacgatc
      541 aggtccacca cacggcgttc gtcgatgagg tagctgggca cctcccctat cgcgccgagc
      601 acgagcgagg gcaccagcgt tgccagaggg tacctccaca ctatggaaag tccccccaac
      661 cagacgcccc tgatggtatt ggtctcgacc atgggcacga gcattctccc ccaacgcgtc
      721 ccgcggggca aacgccaggc gagcgaaatt cgatccggcc agggacccgc cgtgacctac
      781 actagccgcc cgatgagagc gaggcacgga acgcgtcctg ggacgcgtcc cgggaggtgc
      841 cggaagtccc gacgggcgaa ggcagggctg ccgatagccg tcacgctgtc ggatccgccg
      901 ctaacccgtt atgtcgccta ccgagtccag gatccgcaga aggtcgtcgg atgacatatc
      961 ctgcgcttcg cggatgtgac cgcgcatgtt gatccgggcg tccataccgt cggtatagtg
     1021 aacggttatg gggtaatgga tgtcagccac agcctccgaa ccgatctcgt cgttgtcgtt
     1081 gtgcaggagc atgtgcggcc cgaacgcggc gatctcctcc actgcggtct ccggggatgc
     1141 gatcaccaac tcgtgttcgg ggcgtttgcg ccgtatgtag agtgcgagcg cctgacgcta
     1201 catcaggggc gtgatggtta tgagtatgcg catcttatcc ctcgatagaa cctgaagagc
     1261 ctgtcgacaa ccgtgtcgaa cggagaatgg actggaaggt gtaactcccg ggtatcggac
     1321 gccgcgcggg agatagtgcc ccagcgaacg tcccgaccac gcccggcagg cccgtagagc
     1381 gcgccagggt cgtcagtgag ccgggtctcc actccgagcc attgcgtcgt ccaccatgcg
     1441 accagatatc caaccccttc tgaggcccgg tgcccgcttc ggggtgaccc gcttggggaa
     1501 agggaaagga gaagcgagac accagggtat aaggttccta ccgcaaagca ctcccggcct
     1561 aaactcctgt ggggccccgg aacgggatat tggcccgaag tgtgtacgca ccgcacttct
     1621 tccctggcgg tgtaccgtgg atgaaagcgt cccgctgcgg gactactaac cagtagcggg
     1681 gaagcctgct agtcggctcc accgatggcg agcatatcga gggcgatggc cgccgtccag
     1741 gattgttcgc ccgagccgag cggctcgccg gtgaagggct cgaagtattc ggcaaacccg
     1801 cccaccgaaa gctcttccag agtatcccgg cgtatgttct gtgctctggt gctctctccc
     1861 gcgcgcagca gcgaccacca caggagccag ttggcgaccg gccagaccgg cccgcgccag
     1921 tagctacgcg ggtggaaccc gggctctacg gggctcgtcg aagggggcag cgggtagcgg
     1981 aggtcggcgt tgccggcaaa atgctccgat tcgagtgttg caaggagggt gtccaaccgc
     2041 ctgcgatcga ggccgcccgc gatcaggggc gagaatccgg cgatggtgcg cactgggacg
     2101 gattcgccgg ccaacacgtc gtggtccagg catagcccga ggccagggtc ccactgctct
     2161 tccagtcccg cccggccgcg ggcgatccag gctcctatca actctttgtc ttcttcgggg
     2221 gcgtcgacga cctcggagat ctccagcaac gcctcgttgg ccgcgacgag gatagctgag
     2281 aatagtacgt ctttgatcag gaaggggtgc ctctcgtaga tgtcggcctc gtcgtaacga
     2341 gccctcttga gcagctcgag cagccacagg aagcggtcgt actcgctgtc cgtcgggcgg
     2401 tgggagggat cggctacgtg ctggaggtcg taacgggtat aggaaggaac ctcccccacg
     2461 gagatcctct ccagagcagc gtcccagcgc ggggagttgt ccgtgccgct ctcccagggg
     2521 tggtagatgg ttaccaggcc cgacccctcg gggtcgcggg cggtcgccag gtagcggtgc
     2581 caggagaaca acctcctgta gttgctccgg agaaagttgc gggcgcgggt ggcgcgttcc
     2641 tcatccttgc cccttgaggt ctgccagatg cgcctgacgg ctatggcatg gacgggcggc
     2701 tggcaaaggc cgcttgtgtg cttgccctca ggggcgtctt gggagagcgc tgatgagttc
     2761 cagcgctccg cgtccggaaa atagctcctc ggaggcgcct cggggtcgaa gactatgtgg
     2821 ggtaccttgc ctgtcgacca ctgtccgcga aagaggctat ccagctcgtc cgtggcgcgt
     2881 ctgttgtcca cgtgcgcaag accgagcgcg ataaacgccg agtcccagct ccactggtgt
     2941 gggtagaggg agggcgccgg cttggtccag gacttcagcc cgttgcggcg taagacactg
     3001 gtcgcctccg ggttgacgcc gtcgaaacgc cgagatgccg cggccttgga gaggctccct
     3061 tcgaggtctt cctcacgcat aggacggtac tcccaactcc gtcgtttagc ctgacccgct
     3121 caatgtatac aacgaccggc cagccttcaa gaccgggcgg ggaccagatc agtccttcct
     3181 ccggtcagag ccgatgatct catcctgata tgcgtccagg gactcgagga aaccgtcgta
     3241 ggcctccccc gactccctgg agagctcgtg aaaaacctcc cgctgctccc ttacgagttc
     3301 ctgaatgttg ctcagggttc tgcggtgcag ttctgcgtga tcgtcgagcg tctctaccca
     3361 gttctcgaag aacctcctcg acaacttcat accactcttc tgcgcttcaa aggcctggtc
     3421 gacggcagac ttgtaccctt cacgggctat ctcgtcaagc cgctccacga cctcgcgatt
     3481 tcgacgcgct tctctctcgt ccattgcaga cctcctactc ccaaggtctt attctaacct
     3541 ctgtgttcga gtttggagcg cctgcgaagg ttttgggtcg aacgccaccg caccgtttgg
     3601 cgtccgaagc cggtaatctc ggtacgcaat cacggggccg tgtcaggtag acggttatcg
     3661 ggagtgaagg gatcggtccc aggcccgtga tgctgctgac gagacggcta gacctcgccg
     3721 ccaacggatc ctgggaccga agcgttatcg tatagagtga aaagggccct
//
LOCUS       DBNGVD010000588         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4168131, whole genome shotgun sequence.
ACCESSION   DBNGVD010000588 DBNGVD010000000
VERSION     DBNGVD010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4168131"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="surE"
                     /locus_tag="AC16CM_15825"
     CDS             <1..498
                     /gene="surE"
                     /locus_tag="AC16CM_15825"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3141351.1"
                     /translation="GTVAGAMEGIVIGVPGIAVSLSIDRPWHETVEERMPLDAGLHFE
                     TVADFTARLVKVAYKGLPAGRILNVNAPNLPRERLRGARVTRLGRRFYKDELVEVRDA
                     EGRVGYDIYNNPPGRHEEEGTDFSAIENDEISVTPVHLQLTDTTAIEELGAWDLAGLL
                     GNGVD"
     gene            502..1149
                     /locus_tag="AC16CM_15830"
     CDS             502..1149
                     /locus_tag="AC16CM_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR3141352.1"
                     /translation="MLRAALFDFDGTLVDTTEMIYQGMRHATSTVLGREDIPRETLLA
                     NVGQPLPRQMELIDAERAERLLGAYRHHHEENHDALIRAFPDVARDLSRLQAAGVTVA
                     VVTSKRRTSVEMALNNFPDLRGVVDRFVTLEDTREHKPHPEPLWRALELVGDVPKEEA
                     AYVGDSPFDVEAAKAASLTSVAVSWGAFSEDRLREAHPDHLVPDIQSAVDVLLSL"
     gene            complement(1327..>1653)
                     /locus_tag="AC16CM_15835"
     CDS             complement(1327..>1653)
                     /locus_tag="AC16CM_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141353.1"
                     /translation="AAGFAAAATAVHERGSAGFAEIRAKADRLANLLSELPRVTIRSP
                     RPAQSGLISFEIEGVDPGQAIEHLLEQRFILRYVPGQSTYIRASTHLFNTDEELEALK
                     EAVRKL"
BASE COUNT          297 a          572 c          521 g          263 t
ORIGIN      
        1 ggcacggtcg ccggggcgat ggagggcata gtcatagggg ttcccggcat agctgtctcc
       61 cttagcatag accgtccctg gcacgagacg gtcgaggaga ggatgccgct cgacgccggg
      121 ctgcacttcg agacggtggc ggacttcacc gcgcgcctgg tgaaggtggc ttacaagggt
      181 ttgcccgcgg ggcgcatcct gaacgtcaac gcgccaaacc tgccccgcga gaggctacgg
      241 ggcgcgcggg tcacgaggct gggacgaagg ttctacaagg acgagctcgt cgaggtcagg
      301 gacgccgagg ggcgggtcgg ctacgacatc tacaacaacc cacccggccg ccacgaggag
      361 gaggggaccg acttctccgc catagagaac gacgagatca gcgtcacccc cgtccacctc
      421 cagctgacgg acacgacggc catagaagag ctcggggcct gggatctggc cggcctcctc
      481 ggaaacgggg tggactaggt catgctgagg gcggccctct tcgacttcga cggtaccctc
      541 gtagacacga ccgagatgat ctaccagggc atgcgccacg ccacgagcac ggtcctcggc
      601 cgcgaggaca tcccccgcga gaccctcctg gccaacgtgg gccagcccct cccgcgccag
      661 atggagctta tagatgccga gagggccgag cgccttctgg gggcctaccg ccaccaccac
      721 gaggagaacc acgacgccct gatccgtgcc ttccccgacg tcgcgcggga cctctcgcgc
      781 ctgcaggccg ccggcgttac ggtggccgtc gtaacctcca aacgccgcac ctccgtcgag
      841 atggccctca acaactttcc cgacctgcgc ggcgtcgtgg accgcttcgt cacccttgag
      901 gacacacggg agcacaaacc ccaccccgag cccctctgga gggcactcga actcgtcggc
      961 gacgtcccga aagaggaggc cgcctacgtg ggcgactccc cgttcgacgt ggaggcggcg
     1021 aaggccgcca gcctcaccag cgtggccgtg agctgggggg ccttctccga agacaggcta
     1081 cgcgaagccc accccgacca cctcgtcccc gacatccaat cagcggtgga cgtcttgttg
     1141 tctctataag gcaacaggtc gtgggaccgt ctacccggtt tccccaaacc cgccattccg
     1201 ttgagtcagc gggaatccac gcccacacat cgcatcgcca acaacccgaa accacggggt
     1261 cgtgtaacgc ccaccgatgt ccgatccgcc ctccattcct gaagcctgaa acctgatgcc
     1321 ttcttcctac agcttgcgaa cggcctcctt tagcgcttcg agctcttcgt ccgtattgaa
     1381 cagatgcgtg cttgccctga tgtaggtgct ctggccgggt acgtagcgca ggatgaacct
     1441 ctgttccagc aggtgttcga tcgcctgtcc ggggtccacg ccttctatct cgaagctgat
     1501 gagccctgat tgggcgggcc tcggggaccg gatggtgacg cgcggcagtt cagagaggag
     1561 gttcgcgagg cggtcggcct tggccctgat ctccgcgaac ccggcgctcc cgcgctcgtg
     1621 cacggccgtt gccgcggcgg cgaaaccggc ggc
//
LOCUS       DBNGVD010000589         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4896426, whole genome shotgun sequence.
ACCESSION   DBNGVD010000589 DBNGVD010000000
VERSION     DBNGVD010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4896426"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1880
                     /locus_tag="AC16CM_15840"
     CDS             246..1880
                     /locus_tag="AC16CM_15840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3141354.1"
                     /translation="MPLGGLGEIGKNMTAVRQASGMILVDAGMSFPDEEMPGIDLVLP
                     DFTYLREHASELKGIVLTHGHEDHVGALPYLLREFNVPVYATRMTLGLVRSKLQEFGI
                     RRGDLREIKAGDRQNLGGFGLEFIQVNHSIPGAVAIAIRTNAGVIVFSGDYKIDLTPI
                     EGAPMDLGRLAELGKEGVLAYFGDSTNSERPGFVPSERIVGEKLNEVFEKSKGRIIVA
                     SFASHIHRVSQVVEAAKAHDRLVGVSGRSMIRNVQIARELGYLDLPQEMMVDQRDVNK
                     IPDSDIVILTTGSQGEPLAALSRIANGTHRTIEVGQEDTVVIAAHPIPGNERGVSRTI
                     NNLMKRGAHVFYSPLQPVHVSGHAAQEEQKIVLSLLKPRFLVPVHGEFRHLRHHANTA
                     ISLGIPEDHIFILENGSCLEFKNGEARIEESVAAGNFLVDAGAIAESSSGVMRERQQL
                     SEDGMFIVVARINAQNGQLLGPPDVISRGFVSPQSSNGLVDESIGVVSRTLERTASHK
                     VTDWGELKKAIRQDLSGFLSNRTGKRPIILPLIVEV"
     gene            1946..>2638
                     /locus_tag="AC16CM_15845"
     CDS             1946..>2638
                     /locus_tag="AC16CM_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR3141355.1"
                     /translation="MATRKSAPKKTAKKGPPTRGRGKSSRKKGPSPLARLGGSVSSAF
                     TREAVGILLLALGLFYSAAFVSGRGAFLGDAGSWVVNQLLGLPGLALAPAAVVCGLLL
                     LLDRLPGRAIAGMILLVLAAAGTFAATLQRGQMFSARHYPEAGGILGSGFYWGVVGIG
                     GAIAAGLVIGLLYALGLSLVSGVSFATAFGAVGSAGRTLADGIVGLSNRLREGRERPQ
                     ERPARSKASKNSG"
BASE COUNT          488 a          800 c          877 g          473 t
ORIGIN      
        1 gaggccgtga ccgcggatga tatctcgcgc ctcgcccggg agcatctcag aacagaccag
       61 atgtacctct ccgccgtcgg accgaaggag ctcgacctgg gcaggtacct caattagggc
      121 tgtcagcttt cagcgatcag ctttcagcca gagatctgga agggctgata gctgacggcc
      181 gatagccgac ggctttccga ccgaagggag gaaaaacact gttagataaa gagaccttgc
      241 aggtcgtgcc cctgggagga ctgggcgaga tcggcaagaa catgacggcc gtacgtcagg
      301 ccagcggcat gatcctggtt gacgccggta tgtcgttccc ggatgaggag atgccgggga
      361 tcgacctcgt gctgcctgac tttacgtacc tgagggagca cgcgagcgag ttgaagggga
      421 tcgtgctgac gcacggccac gaggaccacg tcggggcgct gccctacctg ttgcgcgagt
      481 tcaacgtgcc tgtttacgcg accaggatga cgctcgggct cgtccgcagc aagctgcagg
      541 agttcggcat caggcggggc gatctgcgtg agatcaaggc cggagacagg cagaaccttg
      601 gcggttttgg cctggagttc atccaggtga accactccat acccggcgcg gtggcgatcg
      661 cgatccgcac caacgccggc gttatcgtgt tctctggaga ctacaagatc gacctgaccc
      721 cgatagaggg cgcgccgatg gacctcgggc gcctggcgga gctgggcaag gagggcgtgc
      781 tcgcctactt cggcgactcc acgaattcgg agcgtcccgg cttcgtgcca tcggagagga
      841 tcgtgggcga gaaactgaac gaggtgttcg agaagtcgaa ggggcgtatc atcgtggcct
      901 ccttcgcctc gcacatacac cgcgtgagcc aggtcgtgga ggccgccaag gcgcacgaca
      961 ggctcgtggg tgtctcggga cggtccatga tccggaacgt ccagatcgcg cgcgagcttg
     1021 gctacctgga cctgccgcag gagatgatgg tcgaccagcg ggacgtgaac aagatccccg
     1081 actcggatat cgttattctt accacgggtt cccagggcga gcccctggca gccctgagcc
     1141 gcatcgcaaa cggtacacac aggaccatcg aggtcgggca agaggatacg gtggtcatag
     1201 cggcccatcc catcccgggc aacgagcgtg gtgtgtcgcg cacgatcaac aacctgatga
     1261 agcgcggcgc gcacgtgttc tacagtcctt tgcagccggt ccacgtctca ggccacgcgg
     1321 cccaggaaga gcagaagatc gtgctctcac tcctgaagcc tcgcttcctg gtcccggtgc
     1381 acggcgagtt caggcacctg aggcaccacg ccaacactgc gataagcctc ggcatccccg
     1441 aagaccacat ctttatcctc gagaacggct cctgccttga gttcaagaat ggcgaggcgc
     1501 gtatcgagga gagcgttgcg gcaggcaact tcctcgttga cgccggcgcc atcgcggagt
     1561 cctcatccgg ggtaatgcgc gagcgccagc agctctccga ggacggcatg ttcatagtgg
     1621 tcgcccgtat aaacgcccag aacggccagt tactcgggcc gcccgatgtg atctcccgcg
     1681 gcttcgttag cccccagagc tccaacggcc tggtcgacga gtctatcggg gtcgtgagcc
     1741 gaaccctgga gaggaccgca agccacaagg tgaccgactg gggcgagctt aagaaggcta
     1801 ttcgtcagga tctctcaggc ttcctgtcga accgcaccgg taaacgcccg ataatcctgc
     1861 cgctaatagt tgaggtttag gtctaaccct ctaccatacc ttgcggttga cggtggacta
     1921 gaggctcata ggctagacta ggggcgtggc tactcgcaag agcgctccga agaagacggc
     1981 gaagaagggc ccgcccacca ggggccgcgg caagtcgtcc cggaagaagg gtccttcgcc
     2041 gctagcccgc cttggcgggt cggtctcgtc agcgttcacc agggaggctg tggggattct
     2101 actcctcgcc ctcggcctgt tttacagcgc tgccttcgtg agcggacgcg gggcgtttct
     2161 cggggatgcg ggatcctggg tcgtcaacca gctactcggg ttgccggggc tggcgctcgc
     2221 gccggcagcc gtggtctgcg ggttgcttct cctgctcgac cggcttcccg gacgggccat
     2281 cgccgggatg atcctcctgg tgctcgctgc ggcgggtacc ttcgctgcca cattgcagcg
     2341 cgggcagatg ttttctgcca ggcactaccc cgaggcgggt gggatcctcg gaagcgggtt
     2401 ctactgggga gtggtcggca tcggcggtgc tatcgcagcc gggctggtga tcggcctcct
     2461 ctacgcgctg gggctctcgc tggtgagcgg cgtgagcttc gccacggctt tcggcgccgt
     2521 aggtagcgcg ggccgaaccc ttgccgacgg tatcgtgggc ctctccaacc ggctacgcga
     2581 gggccgagag cgtccacagg agcggccggc aaggagcaaa gctagcaaga atagtggc
//
LOCUS       DBNGVD010000590         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1618318, whole genome shotgun sequence.
ACCESSION   DBNGVD010000590 DBNGVD010000000
VERSION     DBNGVD010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1618318"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..764
                     /gene="ftsE"
                     /locus_tag="AC16CM_15850"
     CDS             78..764
                     /gene="ftsE"
                     /locus_tag="AC16CM_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR3141356.1"
                     /translation="MIRFNNVTKVYGRDTVALERINLEIEPGEFVFLVGASGSGKSTM
                     MRLILKETEPSAGSIFVNGAKLSAVPRRKVPRLRRNIGCVFQDYKLLPNKTAAENVAY
                     AMEVTGQRRRIIRTKVPQILDLVGLSGKVDKYPDQLSGGEQQRVSVARAFVSQPPILV
                     ADEPTGNLDPKTSVGIMQLLHRINRTGTTVVVATHDREMVDVMRKRVVALEDGHIVRD
                     KVKGAYTDEG"
     gene            754..1641
                     /locus_tag="AC16CM_15855"
     CDS             754..1641
                     /locus_tag="AC16CM_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR3141357.1"
                     /translation="MRGSMGFLFGEALKNIRLNLLMAITAVTTTFVCILVFGLGLLVS
                     SHIQGIIGSVREDVSVEAFMPNETRQEMNTLAQEVKGWPQVTEVTRVSEQEAFAEFRD
                     KFSDQPDLYEDLDQGVLPKSLQIQLEDPSTATAVAERLKQEEGFTEEDLSYPQQTIDR
                     LNSVTSVVIWGLYGATLLFLIASVLLISNAIRLSIFARRKEIEVMKLVGASDGFVRTP
                     FVFEGLLQGLAGSVIAALVVLWINYLFVEWAHDALPFVPIATEAVNAPFLILLLVAVG
                     VAIGVIGSFLSVTRFLRKI"
     gene            1902..>2364
                     /locus_tag="AC16CM_15860"
     CDS             1902..>2364
                     /locus_tag="AC16CM_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR3141358.1"
                     /translation="MLVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDIVLVNARE
                     KNRSLRGEVTRVIGTSEDPRNVYEALFASIGTARTFPDKVNEEAEAVASRGQGERRDL
                     RDLPTVTIDGADAKDFDDAISAERTGDGYRVWVHIADVTHYLEPGSALDKQA"
BASE COUNT          451 a          719 c          768 g          426 t
ORIGIN      
        1 cggtggccgg gtccctggag gggggacttc gaaacccctt cgaggttggc ggcccgcagg
       61 ttaattctgg agaggtcatg atccgattca acaacgtcac caaggtctac ggtagggaca
      121 cggtcgccct ggagcgaatc aacctggaga tcgagccggg cgagttcgtg ttcctcgtgg
      181 gggccagcgg ttcgggcaag tccacgatga tgcgcctgat cctgaaggag acggagcctt
      241 cggcggggtc catcttcgtg aacggcgcga agctctctgc ggtgccacgc cgcaaggtgc
      301 cacgcctgcg ccgcaacata gggtgcgtgt ttcaggacta caagttgctt cccaacaaga
      361 cggcggcgga gaacgtcgca tacgccatgg aggtcaccgg ccagaggcgg cgcattattc
      421 gcaccaaggt gccccagatc ctggacctcg tgggcctctc gggaaaggtg gacaagtacc
      481 cggaccagct ctccggcggc gaacagcaga gggtctccgt ggcccgggcc ttcgtgtcgc
      541 agccgcccat cctcgtcgcc gacgagccga cgggcaacct cgacccgaaa acgagcgtcg
      601 gcatcatgca gctcttgcac aggatcaacc gcactggaac gaccgtcgtg gtcgccaccc
      661 acgacaggga gatggtagac gtgatgcgca agcgggtggt ggccctggag gacggccaca
      721 tcgtccgcga caaggtgaag ggggcttaca ccgatgaggg gtagcatggg cttcctcttc
      781 ggcgaggcgc tgaagaacat ccgactcaac ctgctcatgg ccatcacggc cgtcacgacc
      841 acgttcgtct gcatcctggt cttcggcctc ggcctcctgg tcagctccca catccagggg
      901 ataatagggt cggtgcggga ggacgttagc gtggaagcgt ttatgcccaa cgagacacgc
      961 caggagatga acaccctcgc gcaagaggtg aagggatggc cccaggtcac cgaagtgacc
     1021 agggtttccg aacaggaagc tttcgccgag ttcagggaca agttcagcga ccagccggat
     1081 ctttacgagg acctcgacca gggcgtcctg ccgaagtcgc tccagatcca gctcgaggat
     1141 ccgtccacgg ccaccgcggt cgccgagagg ctcaagcagg aagaggggtt cacggaagaa
     1201 gacctcagct acccgcagca gaccatcgac cgcctcaact cggttacctc ggtggtgatc
     1261 tggggtctct acggcgccac gcttctgttc ctcatagcaa gcgtgctcct gatctcaaac
     1321 gctataaggc tctccatctt cgccaggcgc aaggagatag aggtaatgaa gcttgtcggc
     1381 gcctcagacg gcttcgtccg taccccgttc gtgttcgagg ggctgctcca gggtctcgca
     1441 ggctccgtta tcgccgccct ggtcgtgctc tggataaact acctctttgt agaatgggcc
     1501 cacgatgccc tccccttcgt gcccatcgcc accgaagccg tgaacgcccc gtttctgatc
     1561 ctgctgctgg tcgccgtggg cgtggccata ggggtcatcg gtagcttcct ctcggtaacg
     1621 aggttcctga gaaaaatata gcttcaaggc ttcaggtttc aggctacagg cgtcaggtag
     1681 gtaggttccg atcgggtccg cgagtgcttt gtgggctccg cctcgggcgt catctcggac
     1741 tggtcgggcg gcacccaaac gcccgtagat tccgctcgcg acaagccagg acctggagcc
     1801 tgctgcctgt agcctgaagc ctggagaacg catgctgcgt ggtagactct gggcgtgcct
     1861 tgccagatac tcttgggagc gctctcttga tcctaccggc catgctggtt cgtcggggga
     1921 agtactcagt agcagatccg ctcttcgtgg aagagcggcg tccggtgctg gtcgcacgca
     1981 agcttcgtag gggcgcagag gcgggggata tcgtgctcgt caacgcccgc gagaagaacc
     2041 gctcgctgcg cggcgaggtg acccgcgtca tcgggacgtc ggaggacccg cgcaacgtct
     2101 acgaggcgct cttcgcctcc atcgggacag cccgtacgtt cccggacaag gtgaacgagg
     2161 aggctgaggc cgtcgcaagc agggggcagg gagagcgaag ggacctgcgc gaccttccca
     2221 cggttaccat agacggtgcc gacgccaagg atttcgatga tgccatctcg gcggagcgga
     2281 ccggcgacgg gtacagggtg tgggtgcaca tcgccgacgt gacgcattac ctggaacctg
     2341 gctctgcgct ggacaaacag gcca
//
LOCUS       DBNGVD010000591         2619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3804454, whole genome shotgun sequence.
ACCESSION   DBNGVD010000591 DBNGVD010000000
VERSION     DBNGVD010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3804454"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16CM_15865"
     CDS             <1..219
                     /locus_tag="AC16CM_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141359.1"
                     /translation="GEPDGAILRLVDTLGAGLVVLGSRGLGGIRRAFLGSVSESVMHS
                     AGCPVMIVRRDLPDGADGTENGAEEAPV"
     gene            complement(363..1106)
                     /locus_tag="AC16CM_15870"
     CDS             complement(363..1106)
                     /locus_tag="AC16CM_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3141360.1"
                     /translation="MQFGATAGVFLFALLTAVATGLGALPFLFVKRPPRRWLGASNAI
                     AAGLMLAASFGLLYEGVRYGLMRTLAGALTGLAFIVLVRKLLQRDESPAVFAQMSNMD
                     ARKALLIVGVMTMHSFTEGVGVGVSFGGGIELGLFITLAIAVHNIPEGLAISLVLVPR
                     GLSWSRAALWSVFSSLPQPLMAVPAFVFVETFRTFLPFGLGFAAGAMIWMVFSELVPD
                     ALEEASGNLVAIIVTLSVLAMLTFQVIIR"
     gene            complement(1167..1643)
                     /locus_tag="AC16CM_15875"
     CDS             complement(1167..1643)
                     /locus_tag="AC16CM_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141361.1"
                     /translation="MNNRQQASAGRDRRRPTPYGKILVATDGSDCSMLAAAHAIYLAE
                     SLGARLYAVYAVDAPRAFHIGIHFREAMTELEASGAKAVAAVREMAEERGLECEEIVA
                     EGSPHESIILTSERVDADLLILGSTGMTGLERALLGSVSRRVLLYSGRPVLLVREP"
     gene            complement(1815..>2619)
                     /locus_tag="AC16CM_15880"
     CDS             complement(1815..>2619)
                     /locus_tag="AC16CM_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3141362.1"
                     /translation="CELHVAHFWQTVPTTRFEAMVRAQLRLEARELLEAQEASLREAG
                     AKVSGAHLRLASAVDGILDLAGELGADLLIMGSRGRGPVKRLILGSVSEGVVHQTRVP
                     VLVVRGGDNAWPPGSIVIGDDGSEAAAHAACLALGIGGLFGAGGLLVRTYPRLPEMDP
                     EGRALDARMVDDELRREENILEERARQLRENLGVRSRIRIDVGDPAARLLGAAEERHE
                     GERTLLAVGSRGLGALGRARLGSVSTKVLRAAKGPVLICPGPRARDPQA"
BASE COUNT          445 a          870 c          889 g          415 t
ORIGIN      
        1 ggagagccag atggggcaat attgcgcctc gtcgacaccc tgggggccgg gcttgtcgtg
       61 cttggcagcc gggggctcgg gggcatccgg cgggcatttc tcggcagcgt ctcggagtcg
      121 gtgatgcaca gcgccgggtg cccggtcatg atcgtccgca gagaccttcc ggatggcgcc
      181 gacgggacgg agaacggcgc ggaggaggca cctgtttgag gacgcctggt gacgctaccg
      241 atgtgtaccg ctcggccctg ggctggagag caggggaggg tccacgagcg ggcggtggcg
      301 gatctttgac gtgaggacgc ggggtgagcg cctcgcgggg acgagagctc ggatggattc
      361 catcaacgga ttatcacctg gaaggtcagc atggcgagga cggagagcgt gacgatgatg
      421 gccaccaggt tgccggaggc ctcttcaagg gcgtctggaa cgagctcgga gaagaccatc
      481 cagatcatgg cccccgcagc gaacccgagt ccgaagggga ggaaggttct gaaggtctct
      541 acaaagacga aggcggggac cgccatcagg ggctggggga ggctggagaa cacgctccag
      601 agggccgccc ggctccatga caggccgcgg ggcacgagca ccaggcttat ggctagacct
      661 tctgggatgt tgtgcacggc tatggccagc gtgatgaaga gccccagctc gatcccgccg
      721 ccaaaagaga cccccacgcc gacaccttcc gtgaaagagt gcatggtcat caccccgact
      781 atcaggagcg cctttcgggc gtccatgttg ctcatctgcg cgaagacggc aggggactcg
      841 tcacgctgca gcagcttgcg aaccaggacg atgaaggcaa ggcccgtgag ggcccctgcc
      901 agggtgcgca tcagcccgta gcggaccccc tcgtagagga ggccgaagct agccgcgagc
      961 atcagcccgg cggctatcgc gtttgacgcc ccgagccacc tccgcggtgg ccgcttgacg
     1021 aagaggaagg gcaacgcccc gagcccggtc gcaaccgccg tgagcaacgc gaagaggaag
     1081 actccagccg tggccccgaa ctgcacgtgg ctaaccctgc gtgcgggcca ggccagcgag
     1141 gagcgaaaga gatgggcgga acaccatcaa ggctcgcgca ccaggagtac ggggcgcccg
     1201 gagtagagga gcacccttcg agacacgctc ccgagcaggg cccgctccag gccggtcatg
     1261 cccgtggaac cgagtatcag gaggtcggca tctaccctct cggaggtaag gatgatgctc
     1321 tcgtgcgggg atccctcggc cactatctcc tcgcactcga ggccccgctc ttcggccatc
     1381 tcgcgcacgg cggccaccgc tttcgcgccg gacgcttcga gctccgtcat tgcctcgcgg
     1441 aagtgtattc caatgtggaa agcgcggggt gcgtccaccg cgtagaccgc gtagaggcgg
     1501 gctccgagcg actcggcgag gtagatcgcg tgggcggccg caagcatgga gcagtcggac
     1561 ccgtccgtgg ccacgagaat cttcccgtaa ggtgtcggcc tgcgtctgtc ccggccggcg
     1621 cttgcttgct gtcggttgtt catcttcgtt tctcctctcg ctcgccgccc ttccaccttt
     1681 cattcgtccg caactactcc agacgcacga gggtgcgccc acctttcccg ttaccaccag
     1741 ggctgacaac acgtggcaag cggtccggcc ggccgggtaa gtccggcgcg gctggtgagg
     1801 ggtactcccg acgtctaggc ctgcgggtca cgagctcgcg ggcctgggca gatcagcacc
     1861 ggtcccttcg cggccctgag aacctttgta gagacgctac cgagcctcgc acgccctagc
     1921 gctccaagtc cgcggcttcc cacagcgagg agcgtccgtt ctccctcgtg tcgctcttcg
     1981 gcggcaccga gcagccttgc agcggggtct ccgacgtcta tacggatcct ggatcggacg
     2041 ccgaggttct ccctcaactg cctggctcgt tcctcgagga tgttctcctc gcgcctcagt
     2101 tcgtcatcca ccatgcgggc gtccagtgca cgcccttcgg ggtccatctc aggcagcctg
     2161 gggtaggtcc tgacgaggag cccgccggcc ccgaagagcc cgccgatccc cagggccagg
     2221 caagcggcgt gggccgccgc ttccgagccg tcgtccccga tcacgatact cccgggtggc
     2281 caggcattgt cgccgcccct tacaacgagc acggggacgc gggtctggtg gaccacaccc
     2341 tccgagacgc tgcccaggat gaggcgcttg acgggtccgc gtccccggct gcccatgatc
     2401 aggaggtcgg ccccgagctc cccggcgagg tctagtatgc cgtctaccgc gctggccaac
     2461 cggaggtgcg cgccggagac cttcgcgccg gcctcgcgga gagacgcctc ttgtgcctcg
     2521 agcagctccc gagcctccag ccggagctgg gcccgcacca tcgcctcgaa gcgcgtggtc
     2581 ggcacggtct gccagaagtg tgcaacgtgc aactcgcaa
//
LOCUS       DBNGVD010000592         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1438196, whole genome shotgun sequence.
ACCESSION   DBNGVD010000592 DBNGVD010000000
VERSION     DBNGVD010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1438196"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="AC16CM_15885"
     CDS             <1..361
                     /locus_tag="AC16CM_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3141363.1"
                     /translation="DGTAEGELPTLRTLETPAQLLEQRAASLAGALGSNVPALEVDVT
                     PSVARSGGGTLPTYEISSFAVRLGGTDAEVLAAHLRASDPPVIGRVHEGRVWLDVRTL
                     LPGEEEAVIEAVRNARG"
     gene            354..2213
                     /gene="selB"
                     /locus_tag="AC16CM_15890"
     CDS             354..2213
                     /gene="selB"
                     /locus_tag="AC16CM_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3141364.1"
                     /translation="MGEAVNEADGHRISLTIGTAGHVDHGKTTLVRYMTGTDTDRLEE
                     EHRRGISIVPGYAELDLPGGRRASLVDVPGHERFVKNMVSGSTGVDAFLLVVAADDGV
                     MPQTLEHLDILRLLGVDQGVVALSKTDAVDDETVELAALEIEELMESRGLEVPVVHVS
                     GTTGDGVDDLLRELDGLVGHPARPASGELARLPVDRAFVLRGIGVVVTGTLWSGEIRP
                     GDVLHTTRGLRPRVRGVQNHGHPVEVAYAGARTALDLTGIEISEIEAGEVLLSHPVPA
                     TRDVDARIRLLESAKPLAHGSLVRVHHGTRATNSRLRLAGGREIPPGGNALARLRLDE
                     PFVVLRGDRFVLRSMEGTTVGGGTALDPYPVARRPDLPWLEALESGDAKRAAPLALAR
                     KPREGMTMEELSLALGLPSEEVLRAVEADPELEKVGNLYAPAEEIGAARSRLLEALRR
                     YAAAHPESPRLTVAAARTATGLSVRLADALLESAEGIRSTEAGVGLPQEEIPPELEDE
                     AEALLENLRASGTEPPASGPTPALRLLLKRGEAVELGGSLFAARGAAEAVLERIKTIC
                     REEGDITLAELRDSLSTSRKFAQAWLEYSDAAGVTSRTGDVRVLTRRHRQAVE"
     gene            2232..2320
                     /locus_tag="AC16CM_15895"
     tRNA            2232..2320
                     /locus_tag="AC16CM_15895"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2264..2266,aa:Sec,seq:tca)
     gene            2411..>2852
                     /gene="moaC"
                     /locus_tag="AC16CM_15900"
     CDS             2411..>2852
                     /gene="moaC"
                     /locus_tag="AC16CM_15900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3141365.1"
                     /translation="MSFSHLDSEGAARMVDVGEKAVVRRTAVAGGSVRMSPETVALLR
                     ETALPKGDVLSVARIAGVMAAKKTPELIPLCHGLNLTSVDVDFEVGHDYVGITATARV
                     SGRTGVEMEALTAVSVAALTIYDMCKAVDKGMVVEEIKLLRKTKE"
BASE COUNT          488 a          942 c         1005 g          417 t
ORIGIN      
        1 ggacggtacc gcggaaggag aattacccac cctccgcacg ctagagaccc cagcccaact
       61 tctggagcag cgggccgcca gcctcgccgg ggcgttgggg agcaacgtac ccgcgctgga
      121 ggtcgacgtg acgccttctg tggcccgtag cggcgggggg acgctgccga cgtacgagat
      181 atcctccttc gccgtaaggc tcggtggaac ggacgccgaa gtcctggccg cacacctgcg
      241 cgcctccgac ccgccggtga tcgggcgcgt gcacgaaggg agggtgtggt tggacgtgcg
      301 cacgttgctg ccaggcgagg aagaggccgt gatcgaggcc gttagaaacg cccgtgggtg
      361 aggccgtgaa cgaggccgac gggcacagga tctccctcac catcgggacg gcgggtcacg
      421 tggaccacgg caagaccacg ctcgtccgtt acatgaccgg caccgacacc gacaggctgg
      481 aggaggagca ccgcaggggc atctccatcg tgcccggcta cgcggagctg gaccttcctg
      541 gcggccggcg ggctagcctg gtcgacgtgc ccggccacga gcgcttcgtc aagaacatgg
      601 tctcggggtc aaccggcgtc gacgctttcc tgctggtcgt cgccgccgac gacggcgtca
      661 tgccccagac cctggagcac ctggacatcc tgcggctgct cggcgtggac cagggcgtgg
      721 tcgccctctc gaagaccgat gcggtggacg acgagaccgt agagcttgcc gcgctggaga
      781 tcgaggagct gatggagtcg agggggctcg aagttccggt ggttcacgtt agcggcacaa
      841 caggtgatgg cgtggatgac cttctgcggg agttggatgg gctggtcggt catccggcgc
      901 gtccggccag cggggaactg gcacggttac ccgtggaccg cgccttcgtg ctgaggggca
      961 tcggggtagt cgtgacggga acgctgtgga gcggggagat caggccgggc gacgtgttgc
     1021 acactacgag ggggctgcgc ccgagggtgc ggggcgtgca gaaccacgga cacccggtcg
     1081 aggtagccta cgccggtgcc cgcacggcac tcgacctgac ggggatcgag atctcggaga
     1141 tcgaagccgg tgaggtactg ctctcccatc ccgtcccggc cacccgcgac gtcgacgccc
     1201 gcatccgcct gctggagagc gcgaaacctc tcgcccacgg ctccctcgtg cgggtgcacc
     1261 atggaacccg tgccacgaac tcccgcctca ggctggccgg gggacgggag atcccaccgg
     1321 gcggcaacgc cctcgcccgc ctgcgcctcg acgagccgtt tgtcgtgctg cggggcgacc
     1381 gcttcgtgct ccgctccatg gaaggaacca cggtgggcgg cggaactgcg ctcgacccat
     1441 acccggtggc ccgacgcccc gacctcccct ggctggaagc cctcgaaagt ggcgacgcga
     1501 aacgggccgc tccactggcc ctggcccgca agccccgcga gggcatgacc atggaagaac
     1561 tctcgctcgc ccttggccta ccatcagaag aagtgctccg cgccgtggag gccgaccccg
     1621 aactcgagaa ggtgggaaac ctctacgctc cggcagaaga gatcggggcg gcgcgcagca
     1681 ggctcctgga ggcgctcagg cgttatgccg ccgcgcaccc cgagagcccc cggctcaccg
     1741 tcgccgcggc ccgcaccgcc accggcctct ccgtccgtct cgccgacgcc ttgctcgaga
     1801 gtgccgaggg gatccggtcg accgaagcag gcgtggggct accgcaagag gagatacccc
     1861 ccgaactgga agacgaggcc gaggccctgc tcgaaaacct gcgtgcctcc ggcaccgagc
     1921 cgccagcgtc ggggcccacg ccggcattgc ggctgctcct gaagcggggc gaggccgtcg
     1981 agttgggtgg ctccctcttc gcggcacgcg gggccgcgga ggccgtgctc gagcgcatca
     2041 agaccatctg ccgggaagag ggagacataa cgcttgcaga actgcgggac agcctctcca
     2101 ccagccgcaa gttcgcccag gcctggctgg agtactcgga cgccgcgggc gtcacaagcc
     2161 gcaccggtga cgtgcgggta ctcacccggc gccacaggca ggcggtagaa taatccccgt
     2221 tagccccacc cggaatgcgt gggcctggtg gcccggccgg tcttcaaaac cggtttgcgg
     2281 ccgtaaaacg gccgtggcgg ttcgattccc ccgcattccg cctttagagc ggtcagcttt
     2341 cagcagttag ccttcggctt cttgttctag gctgatagct gaaagccaag cgaccgaagg
     2401 gagcgccacc atgagctttt ctcacctcga cagcgagggc gcggccagga tggtcgacgt
     2461 tggggagaag gccgtcgtgc ggcggacggc cgtcgccggc gggtccgtcc gaatgtcccc
     2521 cgagacggtg gccctgttgc gcgaaacggc gttacccaag ggcgatgtgt tgagcgtggc
     2581 caggatcgcc ggcgtgatgg ccgccaagaa gacgccggag ttaatcccgc tctgccacgg
     2641 gctgaacctt acctccgtgg atgtggactt cgaggtcggc cacgactacg tcgggatcac
     2701 ggccacggcc cgggtgagcg ggaggacggg cgtagagatg gaggcgctca ccgccgtgag
     2761 cgtcgccgcc ctgaccatct acgatatgtg caaggccgtc gacaagggca tggtggtaga
     2821 agagatcaag ttgctccgaa aaaccaagga ac
//
LOCUS       DBNGVD010000593        11698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_162789, whole genome shotgun sequence.
ACCESSION   DBNGVD010000593 DBNGVD010000000
VERSION     DBNGVD010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_162789"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="AC16CM_15905"
     CDS             <1..860
                     /locus_tag="AC16CM_15905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141366.1"
                     /translation="QRMFSAGSMRPEWFFIAEQEGLPVGRFAFWTLPGMEAPFALVLL
                     DVDWEGDYAEVGTRLLEYALDKARALGADDLEHVIDSPPIRPQFQHHAQRRVELLESA
                     GFYRRRETGRYELADAELPASPGRLSYLGLEEVGEDRFIEAMQQVSEGTLDQEILAER
                     ERLGRNKAAREFFDDARSVQHEASWWRLAYAPNGELAGLVMPAEPPGFLTIFYVGVVP
                     EMRGRGYVNDLLATGTETLLRYRGNGDKPLIADTDVSNTPMAAAFERAGWARFAGRQE
                     YVVDLGAGE"
     gene            complement(942..2195)
                     /locus_tag="AC16CM_15910"
     CDS             complement(942..2195)
                     /locus_tag="AC16CM_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141367.1"
                     /translation="MAQWTREQGRGVGGSTNHTAAWLAWSLVALAVALLGIGFVLSLA
                     TSSLVPNLSSGETEDANVVTILITLLPFSVVGAIIASRHPRNAIGWLFCGVGVTVGCN
                     SFAGDYADFWLASGFGTVGLGEMAAWVSSWLWSLLVFAPLSLLLLLFPDGRLPSRRWR
                     PVAWGVLLGSAGGVVGNALKAGPLVDFPQIANPFGVNSPAVGLLGLVGSIVAAGSMVG
                     SAASLIVRLGRVRGEQRQQIKWLAYGGAIMVAGICLGVLVIPYNVPVSIVIMSVSLLG
                     LPVFTGIAIVKHHLYDIDILINRTLVYATLTAMLTLVYFGGVAGLQELLSPVMGERNG
                     LAVVASTLLIAALFNPLRRRIQTFIDRRFYRKKYDARKTLESFSARLRDETDLKTLND
                     ELVDVVRETMQPAYVSLWLRPDQTP"
     gene            complement(2346..5825)
                     /locus_tag="AC16CM_15915"
     CDS             complement(2346..5825)
                     /locus_tag="AC16CM_15915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3141368.1"
                     /translation="MAVSSGGAFAHLHCHSEYSMLDGASRIRDLVSFAKEQGMPGLAL
                     TDHGVMYGAVKFYKEARDAGIKPLMGCEVYVTADRKDRSRAPYYHLTLIARTAEGYRN
                     LMKLSTSGFLEGFYYKPRVDMEMLRKHGRGVICLSGCLSAEVPTRILEGRPDEARKLL
                     FEYADIFDDVYLELQDHGIPEQRRVNEGLIRLHKETEIPLVAANDSHYTTRNDARMHD
                     VLLCIGTGKFYNDPKRMKFSGEEFYVKTVEEMARIFPDHPEALENTVKVVETVEDVGI
                     ELGKTRLPNFPKPEGYTADQYLREQCERGLVRRYGGRARTQEVRRRLEFELETIGKMG
                     FADYFLIVWDFVKYAKDKKIAVGPGRGSAAGSIVAYALEITDLDPLKYSLLFERFLNP
                     DRINMPDVDIDFSVSGRSEVMRYVTDKYGGHEHVAQIITFGTIGAKAAIRDSGRIYQY
                     PYSDTDKLAKFIPEKPVGTTLRDVLRPEDGSYVAGEKHPGPAREIMQFVARDEAAKQV
                     LDTAFEIEGFARHAGTHAAGVVISEEPLTDIVPLQRVARDETSVMVQHPMSDVEALGL
                     LKVDFLGLRNLDVIEETLETIRQSGGEEPDIRTIPLDDEKALRLFARGDTFGVFQFES
                     SGMQRMLQEVRPDRFDDLVALNALYRPGPMDYIPNFKRGKHDPDSVKYLDERLKPILE
                     PTYGVAAYQEQLMEISKTLGGFTPGEADTLRKAIGKKNAAMLATLRDKFMSGCAESGV
                     SAEGAGELWNWMEKAGGYSFNKSHSACYSFLAFQTAYLKAHYPEAYMAALMSSVMNTK
                     DRVPQYVAEARAMKIEVLPPDVNESGRRFTVVGGTIRFGLSAVKNVGDSCVDSIIAAR
                     QEGGPFEDIFDFCERVDSKTYNKRTLESLIKCGAFDNVGPSRAAMVAVHARAVERSTR
                     GLKGASEDQFCMFDAAELVPPKPAFPDVEQDKRTDLEWEKETLGLYVSDHPLRPVLHK
                     LKKHTNTTVSELEGFRDGAIVWVGGLATSVRTNTTRKGDMMAMLQLDDTRGLAEVMVF
                     PRVYAKCADCVREDAVLKVKGKVEMKEGIPRIVALEMEELHLEPGADPLYLDAGVFVG
                     LSHEVAREAFGLIRQHPGECPLFLVSGDGRSERIAGIHDASDLHAELKQLLGPRCLSY
                     APPTPEPEMEQVS"
     gene            complement(6160..6408)
                     /locus_tag="AC16CM_15920"
     CDS             complement(6160..6408)
                     /locus_tag="AC16CM_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141369.1"
                     /translation="MARGASLTRSQGRRDGPVKRALQATVMGVTLLFVVSALTVSCAL
                     VVYTAWRHDFAHGLFALLFAVLSAQIALNWFLWKDDEE"
     gene            complement(6413..6721)
                     /locus_tag="AC16CM_15925"
     CDS             complement(6413..6721)
                     /locus_tag="AC16CM_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3141370.1"
                     /translation="MPIYEYKCENGHVFDVMQKLSEDPLTTCVECGAPVRKVLHPVGI
                     SFKGSGFYSTDYSNNSKRETATKDDSQSSNGDSGKKDKPKDKSSEKSAGTSTSTGKKD
                     "
     gene            complement(6793..7068)
                     /locus_tag="AC16CM_15930"
     CDS             complement(6793..7068)
                     /locus_tag="AC16CM_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141371.1"
                     /translation="MPESKKRSFWDRLFNRDRPSQREERVLEYIMHRVGEGAHLGDIV
                     NEEYVRRNASREEVEEICARPELIQTAREHLKEDFGSGDLDPTRRPK"
     gene            complement(7175..7663)
                     /locus_tag="AC16CM_15935"
     CDS             complement(7175..7663)
                     /locus_tag="AC16CM_15935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141372.1"
                     /translation="MLVRSATEEDVPLILALIRELAEYERLAHEVIATEKGLRGWLFG
                     ERQVAEALIGELDGHPAAFALFFHNFSTFLGKPGIYLEDLYVRPGFRGRGMGKAMMVR
                     LARLARERGCGRLEWSVLDWNEPSIGFYESLGALAMTGWTVHRLSGEALDGLPEKTET
                     DA"
     gene            complement(7681..8646)
                     /locus_tag="AC16CM_15940"
     CDS             complement(7681..8646)
                     /locus_tag="AC16CM_15940"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3141373.1"
                     /translation="MGRGYTVAVVGAGMVGDRLVAELRRRDFPIEDLRVLARSARTAV
                     LAGETFEVGVAEPEAFDGVDFAFFAGTEGEKGAAVQLSQEAISRGAVVIDNGSDFRLD
                     PDVPLVVPEVNAGAAEGHDGLIANANCSTIQMVVTLAPLAREFGIEKVVVSTYQAVSG
                     SGRGGVEALENGREDVYPKPIHGNAIPLIGSVGEDGRTTEETKMVRETRKIMDLPDLE
                     VHATAVRIPVHTGHSESVYVELARETTLEEVADVLSSAPGVTYSGDADDFPTPLEHAG
                     DSGVHVGRLRVSGNVVEYWCVSDNLLKGAATNAVQIAESLVGARV"
     gene            complement(8680..9741)
                     /locus_tag="AC16CM_15945"
     CDS             complement(8680..9741)
                     /locus_tag="AC16CM_15945"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase family protein"
                     /protein_id="HIR3141374.1"
                     /translation="MKRLEIVQVGLGHVGRAVAQIVLEERKRWLGDGIEVKYRAVSDT
                     SGALSGEELLPQALRLKEEGGSLTQLGVEPLEDVLGSAPEPGFARVVVDVAVHGGTFD
                     IDLLGIRGGAYLVLSNKGPLSGSTAQYEQLRRMIPERLRHEATVGAGMPIISTIGGLL
                     ETGDEILEIQASPSGTLGFIMSAVEDGRSFSEAVGEAVDLHYAEPDPRDDLSGLDVAR
                     KAIILARMMGREIEPEEIPSESLVPEGLQDVPLDEFMRHLPEHDEAFAARLLAVEEHH
                     MLRYLVRIPQNGPVRVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTLTVRGPGAGP
                     ERTASGVVWDLLDIAHKAL"
     gene            complement(9738..10964)
                     /locus_tag="AC16CM_15950"
     CDS             complement(9738..10964)
                     /locus_tag="AC16CM_15950"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3141375.1"
                     /translation="MERPLVIKFGGTSVGGGGEFARAARIVADAAAKRPVVVVVSAMG
                     GTTDTLLGYARMSSGWVRKTLQQQTHGAPVSELRRSLAERHLLAAREAVDPERLPEVE
                     GEIVDLLGELERAIEAPSGNLKARRDEIAVYGERLSSRILAGAISKQGVPAAAVAADP
                     IATNGNFGEAEVDVAATREGAARHVAPLLDRGMVAVVPGYVGRSPGGEPTTLGRGGSD
                     LSATVLGRALGSEEVWIMSDVDGVLDADPRLVPDASLMPRLSYREAGVFAGLGAKVLH
                     PRTMEPAEEAGIEVYVRNTFAPEGAGTHVTGFDPGSGVRCVALRQNVAMEIPCTDGRE
                     GRAAMVVCIGSPEEDDLKRGRRLLREAGIPVQHFGIASAGLVFVVAADVGLDALRALH
                     AGLIPPGARYAGEEVA"
     gene            complement(10970..>11698)
                     /locus_tag="AC16CM_15955"
     CDS             complement(10970..>11698)
                     /locus_tag="AC16CM_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3141376.1"
                     /translation="TLPSPYLVNPLEHGADIVVHSATKFIGGHGTSIGGVIVDGGRFP
                     WDNGKFPGFTEPDPSYHGLEIYPTLGELSFILKARVQMLRDWGPAISPFNSFLFLQGL
                     ETLPLRMERHSTNAQAVAEFLRDHPKVNWVNYPGLEAHPAHELAAKYHREGLFGAILG
                     FGIDGGLETGKRFIDGLQLHSLLANVGDAKSLVIHPASTTHSQLSVDEQKATGVTDDY
                     VRLSVGLEDIDDILYDLDTALNRA"
BASE COUNT         2004 a         3878 c         3658 g         2158 t
ORIGIN      
        1 tgcaacgcat gttctccgcc ggctcgatgc gccccgaatg gttcttcatc gccgagcaag
       61 aaggccttcc cgtcgggcgc ttcgcgttct ggacgctacc cggcatggag gctcccttcg
      121 ccctcgtact gctggacgtc gactgggagg gcgactacgc ggaggttggc acgcgcctgc
      181 ttgaatacgc cctggacaaa gcgcgtgccc tcggggccga cgacctggaa cacgtcatag
      241 actccccgcc catacgtccg caatttcagc accacgcgca aaggcgggtc gagctcctcg
      301 aaagcgccgg tttctaccgc cggagggaga cgggccgcta cgagctcgct gacgccgagc
      361 tgcccgccag cccgggacgg ctctcgtacc tcgggttaga ggaggtcggc gaagacaggt
      421 tcatcgaggc catgcagcag gtctcagaag gcaccctcga ccaggagatc cttgccgagc
      481 gcgagaggct tggccggaac aaagcggcgc gggagttttt cgacgatgcc cgcagcgtcc
      541 agcacgaggc ttcctggtgg cgactggcct acgcgccgaa cggcgagctc gcaggcctcg
      601 tgatgccagc ggagccccct ggcttcctga ccatcttcta cgtaggcgtg gtgcctgaga
      661 tgaggggacg ggggtacgtg aacgacctgc tcgccaccgg caccgaaacg ctcctgcgct
      721 acaggggaaa cggcgacaag cctctaatag cggacaccga cgtttcaaac accccgatgg
      781 cagccgcctt cgagcgcgca ggatgggcca ggttcgccgg aaggcaagaa tacgtcgtgg
      841 atctcggcgc tggcgaatag tatacgtcag caccccctga cgccgacgca tcctccccga
      901 tcttcgcccc agcctgggga ttgctaggct ggctgctccc cctagggggt ttgatcggga
      961 cgaagccaca aggacacgta cgcgggctgc atggtctccc tgaccacgtc aaccagctcg
     1021 tcgttgagtg tcttcaggtc cgtctcgtcc ctgagccttg ccgagaagct ttcgagggtc
     1081 ttcctggcat cgtacttctt cctgtagaag cgcctgtcga taaaagtctg gatgcgcctc
     1141 cgcaggggat tgaacagggc cgctatcagc agggtcgagg cgactacggc gagcccgtta
     1201 cgctctccca tcaccggcga gagcagttcc tggagcccgg ccacgccccc gaagtacacg
     1261 agggtaagca tcgcggtgag cgtggcgtag acgagggttc ggtttattag gatgtcgatg
     1321 tcgtagaggt gatgcttcac gatcgcgata cccgtaaaga ccggcaaccc gagcagggag
     1381 acggacatta ttacgatgct caccggcacg ttgtagggta tgaccaggac gccgaggcag
     1441 atacccgcga ccatgatcgc gcccccgtaa gcgagccact tgatctgctg tcgctgctcg
     1501 ccccgcacgc gcccgagcct gacgatgagc gaggccgccg acccaaccat ggagcctgcg
     1561 gccacgatgc tgccaaccag ccccaggagc cccacggcgg ggctattcac gccgaagggg
     1621 ttcgcaatct gcgggaaatc tactagcggt ccggccttca gcgcgttgcc aaccacgcca
     1681 ccggcgctgc ccagcaagac gccccaggcc acgggccgcc atcggcgtga gggtagacga
     1741 ccatctggga acaacagcag cagcaagctc aggggagcga acaccagaag ggaccacagc
     1801 cacgaagaca cccaggccgc catttctccg aggccaaccg tgccgaaacc gctcgccagc
     1861 cagaagtcgg cgtaatcacc cgcgaaggag ttgcagccta ccgtgacgcc cacgccgcaa
     1921 aacagccacc ctatcgcgtt gcgggggtgg cgggaggcga taatggcacc cacgaccgag
     1981 aacggcagca gcgtgatgag gatggtgacc acgttggcat cttccgtctc gccggatgag
     2041 aggttcggaa ccagggagct cgtcgcgagg gacagcacga acccgatccc aagcagcgcc
     2101 accgccagcg ccaccagaga ccaggccagc caggcggcgg tgtggttggt actccctccg
     2161 acccctcggc cctgctcgcg cgtccattgt gccatagcgt cacactaacc cggtaccgtc
     2221 tctagagcgt tacggtaacc gttacgaggc caacctatgc gcgaagtctg caaaaatctt
     2281 gccggtcgag gtcgctggtc ttctgtgagc gcggttcacg tgctaccagc ggccggaccg
     2341 ctctactacg agacttgctc catctccggc tcgggtgtgg gtggggcgta agagaggcat
     2401 ctggggccta gcagttgctt gagttcggcg tgtaggtccg aggcgtcgtg gatgccggcg
     2461 atccgctccg accttccgtc gccggagaca aggaagaggg ggcactcgcc aggatgctgc
     2521 cggatcaacc cgaatgcctc acgcgccacc tcgtgcgaga ggccgacgaa gacgcccgcg
     2581 tcgaggtaga gggggtcggc acccggctcc agatgcagct cttccatctc cagcgccact
     2641 atgcgcggga taccctcttt catctcgacc ttgcccttga ccttgaggac ggcatcctcg
     2701 cgcacgcagt cggcacactt ggcgtagaca cgcggaaaga ccatgacctc ggccaggcca
     2761 cgcgtgtcat cgagctgcag catggccatc atgtcgccct tgcgcgtggt gttcgtacgc
     2821 accgaggtgg cgagcccccc gacccacacg atggccccgt cccgaaagcc ttcgagctca
     2881 gagaccgtgg tgttcgtgtg cttcttgagc ttgtgcagga cgggccgcag gggatggtcg
     2941 gagacgtaga ggcccagggt ctccttctcc cactccaggt cggtgcgttt gtcctgctcg
     3001 acatcgggga acgcgggctt gggcggcacg agttcggccg cgtcgaacat gcagaactgg
     3061 tcctcgctcg cccccttcag acctcttgtg gagcgttcga cggcccttgc gtggacggct
     3121 accatggccg cgcgtgaagg cccgacgttg tcgaaggcgc cacactttat gagggattca
     3181 agggtgcgct tgttgtacgt cttggagtca acccgctcgc agaagtcgaa gatatcctcg
     3241 aagggaccgc cttcttgcct ggcggcgatg atcgagtcca cgcatgagtc gcccacgttc
     3301 ttgacggccg agagcccgaa ccggatggtc ccacccacca ccgtgaaccg acgaccgctc
     3361 tcgttcacgt cgggcgggag gacctctatc ttcatggccc tggcctcggc aacgtactgg
     3421 ggtaccctgt ccttggtgtt catcacgctg ctcatcaggg cggccatgta cgcctctggg
     3481 tagtgggcct tgaggtaggc cgtctggaag gccaggaacg agtagcaggc gctgtggctc
     3541 ttgttgaacg agtagccacc ggccttctcc atccagttcc acagctctcc ggcaccctcg
     3601 gcagagacgc cgctctccgc acaaccggac atgaacttgt cccgaagggt cgcgagcatg
     3661 gcggcgttct tcttgcctat ggccttgcgc aacgtgtcgg cctcgccggg ggtaaagcct
     3721 ccgagggtct tggagatctc catcagctgc tcctggtagg cagccacgcc gtaagtgggc
     3781 tccaggatgg gcttgaggcg ctcgtcgagg tacttgacgc tatcgggatc gtgcttgcca
     3841 cgcttgaagt tcgggatgta gtccatcgga cccggcctgt agagcgcgtt gagcgctacc
     3901 aggtcgtcga agcggtctgg cctgacctcc tgcagcatcc gctgcattcc gctcgactcg
     3961 aactggaaaa ccccgaacgt gtcgcccctg gcaaagaggc gcagggcctt ttcgtcgtcg
     4021 agggggatgg tgcgtatgtc gggctcctcc ccaccgctct ggcgtatcgt ctccagggtc
     4081 tcctcgatca cgtcgaggtt gcgcagcccc aggaagtcca ccttgagcag gccaagggcc
     4141 tcgacgtcag acatgggatg ctggaccatg accgaggttt cgtccctggc gacgcgctgc
     4201 agcggcacga tgtccgtcag cggctcctcg gagatgacga cgcccgcggc gtgggttccc
     4261 gcgtggcggg cgaagccttc tatctcgaac gccgtatcca gaacctgctt ggccgcctcg
     4321 tccctcgcga cgaactgcat gatctcccgc gcgggccccg gatgtttctc accggccacg
     4381 taggagccgt cctccgggcg caacacgtcc ctgagtgtgg tcccgaccgg cttctccggg
     4441 atgaacttgg cgagcttgtc cgtgtccgaa taggggtact ggtaaatccg ccccgagtcc
     4501 ctgatggcgg ccttggcgcc gatggttcca aacgtgatga tctgtgccac gtgctcgtgc
     4561 ccaccgtact tgtccgtgac gtagcgcatc acctcggagc gccccgagac cgagaagtct
     4621 atgtcaacgt ccggcatatt gattcggtcg ggattgagga accgctcgaa gagcagggag
     4681 tatttcaacg ggtcgaggtc cgttatctcc agcgcgtagg cgacgataga accggccgcc
     4741 gaaccgcgtc ccgggccaac cgctatcttc ttgtccttgg cgtacttgac gaagtcccag
     4801 acgatgagga agtagtcggc gaagcccatc ttcccgatgg tttcgagctc gaactcgagg
     4861 cgcctcctta cctcctgcgt cctggccctg ccaccgtaac gccgcaccag acctcgctcg
     4921 cactgctccc tgaggtactg gtccgccgtg tacccctcgg gctttgggaa gtttggcaga
     4981 cgggtcttgc cgagctcgat ccccacgtcc tccaccgtct ccacgacctt gacggtgttc
     5041 tcgagggcct ccggatgatc ggggaagatg cgggccatct cctcgacggt cttcacgtag
     5101 aattcctcgc cggagaactt catgcgcttg gggtcgttgt agaacttgcc ggttccaatg
     5161 cacagaagca cgtcgtgcat tctggcgtcg ttcctcgtgg tgtagtgaga gtcgttggcg
     5221 gcgacgagcg ggatctcggt ctctttgtgg agcctgatca ggccctcgtt cacccgccgc
     5281 tgctcgggga ttccgtggtc ctgcagctcc aggtacacgt cgtcgaagat gtccgcgtac
     5341 tcgaagagca acttgcgcgc ctcgtctgga cgtccctcga gtatccgcgt cggcacctcg
     5401 gccgagaggc agccagagag gcagatgacc ccgcgcccgt gcttcctgag catctccatg
     5461 tcgacccgcg gcttgtagta gaacccttcg agaaacccag acgtggagag cttcataagg
     5521 ttcctgtatc cctcggccgt cctggcgatg agcgtcagat gatagtaggg ggccctgctc
     5581 cggtctttcc tgtccgccgt aacgtagacc tcgcagccca tcaggggctt gatgccggcg
     5641 tccctggcct ccttgtagaa cttgacagcc ccgtacataa ccccatggtc tgtcagggcg
     5701 aggcctggca tgccctgctc ttttgcgaag gagacgagat ccctgatccg gctggccccg
     5761 tcgagcatcg agtactccga gtgacagtgc aaatgcgcaa aagcgccacc cgacgaaacc
     5821 gccaatctgc ctccctgtac tcagggcttc ggcgacgccc cggtggaacg ccgccactcg
     5881 cctagttctc cccgtcgtct cccgacgtca gctactataa ggcacagccc cgccgattgt
     5941 ccaggctccc tcgaccacag cttgaggcat attttgcagg acttttcagg gggagctttc
     6001 agctttcggg ggtcagatat cagctattag ggccgcagcg cagccctcgt tccaggcgta
     6061 tccacgggcg tggagccagt atatatggaa cgattggcag gcgtcagaaa gtcggctatt
     6121 tccgtttacg actgaaagct aaaagctaag agctgatggc tactcttcgt cgtccttcca
     6181 cagaaaccag ttaagcgcta tctgggcgct gagtacggcg aagagcaatg cgaagaggcc
     6241 gtgtgcgaag tcgtggcgcc aggcggtgta gacgaccagg gcgcaggaga cggtcagggc
     6301 ggagaccacg aagaggagcg ttacgcccat aaccgtggct tggagggctc gtttgaccgg
     6361 gccgtcgcga cggccctggc tccgggtcag gctggcgccg cgcgccatct tttcagtcct
     6421 tttttcctgt actggtcgag gttcccgcgg acttttcgct cgatttatcc ttgggcttgt
     6481 ccttcttgcc cgagtcaccg ttcgagctct gcgagtcgtc ttttgttgcg gtctctctct
     6541 tggaattgtt gctgtaatcc gtcgagtaga aaccagagcc cttgaacgag atgcccacgg
     6601 ggtggaggac cttcctgacc ggggccccgc actcgacgca ggtggtcagg gggtcttccg
     6661 agagtttttg catcacgtcg aagacgtgtc cgttttcaca cttgtattcg tagattggca
     6721 tccagcctcc agtttaaatg ctcgtgggta caaattatac aggctggacg atatctcggt
     6781 tctggaggtc gactatttcg gacgtctggt cgggtcgaga tcccccgatc caaaatcctc
     6841 cttgaggtgt tcgcgggccg tctggatgag ctcgggacgg gcgcagatct cctcgacctc
     6901 ctcgcgggag gcgttgcgcc ttacgtactc ctcgttcacg atgtcgccga ggtgtgcacc
     6961 ctcccccacg cggtgcatga tgtactcgag tacccgctct tcacgctgcg aaggcctgtc
     7021 tctgttgaac agccggtccc agaaagaccg cttcttgctc tctggcatga ctcactcctc
     7081 gtctcagggt cctcttgcgc aagtgtaacg aaccgcacgt ccctagtata gccccggagt
     7141 ccggccccgg aaaggtcttg gcgcgcgtgc gtcctcaagc gtcggtctcg gtcttttcgg
     7201 gcagcccgtc cagtgcctct cccgatagcc tgtgtacggt ccagcccgtc atagccaggg
     7261 cgccaagcga ctcgtagaac ccgatggagg gctcgttcca gtcgaggacg gaccattcca
     7321 ggcgcccgca accgcgctct ctggccaacc tggccaggcg aaccatcatg gcttttccca
     7381 tcccccttcc ccggaaccct gggcgcacgt agaggtcttc caggtagatg cccggcttgc
     7441 cgagaaaggt ggagaagttg tggaagaaga gggcgaacgc tgcagggtgg ccatccagtt
     7501 ccccgatcag ggcttccgcc acttgccgtt cgccgaacaa ccagccgcga agccctttct
     7561 ccgtcgctat cacctcgtgg gcgagcctct cgtactcggc cagctcgcgg atgagggcca
     7621 ggatcagggg cacgtcctct tcggtagcgc tccgaaccag catcccagtc ccgctggtgg
     7681 ctagacccgg gcgccgacga gcgactcggc gatctggacg gcgtttgtgg ccgccccctt
     7741 gagcaggttg tccgagacac accagtactc cacaacgttc cctgagaccc gcaaccgccc
     7801 aacgtgtacc ccggaatcgc cggcgtgctc caggggggtt gggaaatcgt ccgcgtcccc
     7861 ggagtacgtg acgccgggcg cgctcgacag tacgtcagcc acctcctcca gcgtggtctc
     7921 cctggcgagc tctacgtaga cgctctcgga atggccggtg tggacgggta tgcgtacggc
     7981 ggtcgcgtgg acctcgaggt cggggaggtc catgatcttg cgcgtctccc tcaccatctt
     8041 ggtctcctcg gtggtccgcc cgtcctcccc aacagagccg ataaggggga tggcgttgcc
     8101 atggatgggc ttcgggtaga cgtcctctcg cccgttctcg agcgcctcca ccccaccacg
     8161 gcccgacccg gagaccgcct gataggtcga cacgaccacc ttctcgatgc cgaactcgcg
     8221 ggccagcggc gcgagcgtta caaccatctg gatggtcgaa cagttggcgt tcgcgatgag
     8281 gccatcgtgt ccttccgccg ccccggcgtt tacctctggc acgacgagcg gcacgtcggg
     8341 gtcgagcctg aaatcactgc cgttgtctat tactacagcc ccccgtgaga tggcctcctg
     8401 ggagagctgg acagcggcgc ccttctcccc ctcggtgcca gcgaagaacg cgaagtccac
     8461 gccgtcgaag gcctccggct cggccacccc gacctcgaag gtctccccgg ctagcaccgc
     8521 cgtccgcgcg ctcctggcca gaacccgcaa atcctcgatc ggaaagtcac gccgcctcag
     8581 ctcggcgacc agcctgtcgc ccaccatccc ggcgcctact accgctaccg tataacctct
     8641 gcccatatca ccctccgcca aatatctgat gcctgaaacc tacagcgcct tgtgcgcgat
     8701 atccagaagg tcccagacca ccccgctcgc cgtgcgttcc gggccggcgc cgggaccacg
     8761 aacggtgagg gtaacatccg agtagcggcg ggtgcggaaa tcgaacacgt tctcgggacc
     8821 gcttatgggg ccgaaagggc tctccacggg ggtgtcctgc aggccgaccc tgacggggcc
     8881 attctgcgga atgcgtacca ggtagcggag catgtggtgt tcttcgaccg cgagcaggcg
     8941 cgccgcgaac gcctcgtcgt gttcagggag gtgtcgcatg aactcgtcta gcgggacgtc
     9001 ctgcaagccc tctgggacca gagattcgga ggggatctct tccggttcga tctcgcggcc
     9061 catcatgcgg gccaggatga tggctttcct ggccacatcc aggccagaga ggtcgtcgcg
     9121 ggggtcgggc tcggcatagt ggaggtccac ggcctctccc accgcctcgg agaatgacct
     9181 cccatcctcc accgcgctca tgatgaagcc aagggttccg ctcgggctgg cctgtatctc
     9241 tagtatctcg tcgcccgtct caagaaggcc gccaatggtg gagatgatcg gcatccccgc
     9301 ccccaccgtg gcttcgtgcc tgagacgctc cgggatcatg cgccttagct gctcgtactg
     9361 ggccgtgctg ccggagagcg ggcccttgtt ggagaggacg aggtacgcgc cgccgcggat
     9421 gccgagcagg tcgatgtcga aggtgccccc gtgcaccgcc acgtccacca ctacccgcgc
     9481 gaaacccggc tcgggggccg agccgagcac gtcctccagc ggctcgacgc cgagctgggt
     9541 gaggctgccg ccttcctcct tcaggcgtag ggcctgggga agcagctcct cgcccgagag
     9601 ggcgcccgag gtgtccgaga cggcgcggta cttgacctcg atgccgtcgc ccagccagcg
     9661 cttccgttct tcgaggacga tctgggccac agcccggccc acatgcccca ggccgacctg
     9721 gactatctcc agccgcttca cgccacctct tcgccggcgt atcttgcccc gggcgggatc
     9781 aggccggcgt gcaacgcccg caaggcgtcg aggccgacgt cggcggccac gacgaagaca
     9841 aggccggccg aggcgatacc gaagtgctgc accgggatgc cggcctcccg caagaggcgt
     9901 cgcccgcgct tcaggtcgtc ctcctcggga gaacctatgc acacgaccat cgcagcccgc
     9961 ccctcccggc cgtcggtgca ggggatctcc atggcgacgt tctgcctgag cgcgacgcaa
    10021 cgtactcccg acccgggatc aaagcccgtg acgtgggttc ccgcgccctc tggcgcgaac
    10081 gtgttgcgca cgtagacctc tataccggcc tcctcggcgg gctccattgt ccttggatgc
    10141 aacaccttgg cgccgagccc ggcgaagacg ccggcctccc tgtacgagag gcgtggcatg
    10201 agtgaggcgt ctgggaccag ccgcgggtcc gcatcgagta cgccgtccac gtcggacatg
    10261 atccagacct cttcagatcc gagcgccctc ccgagcaccg tggccgagag gtcagaacct
    10321 cctctgccga gcgtggtcgg ctctcccccc ggcgagcgcc ccacataacc gggcactact
    10381 gccaccatgc cgcggtcgag caacggtgcg acatggcggg ccgcgccctc gcgggtggca
    10441 gcgacgtcca cctcggcctc accgaagtta ccatttgtgg ctatggggtc ggccgccacg
    10501 gcggcggccg gcacgccctg cttgctgatc gcgcctgcca gtatccggga ggagaggcgt
    10561 tctccgtaga ccgcgatctc atccctccgc gccttgaggt tgccggaggg ggcctctatg
    10621 gcccgctcga gttcgccgag caggtccacg atctcgccct ccacctcggg cagccgctcc
    10681 gggtcgacgg cctcacgggc ggccagcagg tggcgctcgg cgagtgagcg ccggagctcg
    10741 gagacggggg ccccgtgcgt ctgctgctgc agcgtctttc tcacccatcc agagctcatc
    10801 ctggcgtagc cgagcagggt atccgtggtg ccgcccatcg cagagaccac gacgaccacg
    10861 ggccgtttag cggcggcgtc ggcgactatg cgggcggcgc gggcgaactc gccgccgccg
    10921 cccacagagg tgcccccgaa ctttatgacg aggggtcgtt ccaaccgggt cacgccctgt
    10981 tcaaggcggt atcgaggtcg tacaggatgt cgtcgatgtc ctcgaggccg acggagagcc
    11041 gcacgtagtc gtcggtaacg ccggtcgcct tctgctcgtc gacggagagc tgcgagtggg
    11101 tggtcgaggc cgggtggatt accaggctct tggcgtcgcc cacgttcgcg agcaggctgt
    11161 gcaactgcag accgtcgatg aagcgcttgc ccgtctcgag gccaccgtcg atgccgaagc
    11221 cgaggatcgc cccgaagagc ccttcacgat ggtacttcgc cgccagctcg tgcgccggat
    11281 gggcctcaag gcccggatag ttcacccagt tcaccttcgg gtggtcccgc aagaactcgg
    11341 ccaccgcctg ggcgttcgtg gagtggcgct ccatgcgcag gggcagcgtc tccaggccct
    11401 gcaggaagag gaagctgttg aacgggctta tggccgggcc ccagtccctc agcatctgga
    11461 cccgcgcctt caggatgaac gaaagctcgc ccagggtcgg gtagatctcc agcccgtggt
    11521 aggaagggtc tggctcggtg aagcctggga acttgccgtt gtcccacggg aaacggccgc
    11581 cgtccacgat cacgcccccg atggacgtgc cgtgcccccc tatgaacttg gtcgccgagt
    11641 ggacgacgat atcggcgccg tgctccagcg ggttgaccag gtagggggat ggcagcgt
//
LOCUS       DBNGVD010000594         3762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_709297, whole genome shotgun sequence.
ACCESSION   DBNGVD010000594 DBNGVD010000000
VERSION     DBNGVD010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_709297"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..919)
                     /locus_tag="AC16CM_15960"
     CDS             complement(92..919)
                     /locus_tag="AC16CM_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141377.1"
                     /translation="MSANSGTPVVNTPQIMSAGIAATGASLVTSKFGVAGTLLGAALT
                     AMLVTGLTSILRAYLETLSGHVRGMPKKLRTRREQQGGATMPGRPDLRDNFAGRMRAA
                     LGWFSNLPTLRRRSILMKGFLAAVVAFLLCMGLVWGTEKVIGNSLSCGLWSECPYGAT
                     PGIHPMGYGGTGANSSFSGGSAAGTETPSLVDRVRQNGGNPLGGNPFGGGDQPVVGNP
                     GGGAVPSASPDPAASPEPGGNGSASPGAGQGADSGGSASPAASQKEAPSSASPAAGQ"
     gene            1064..1510
                     /locus_tag="AC16CM_15965"
     CDS             1064..1510
                     /locus_tag="AC16CM_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141378.1"
                     /translation="MTEPDTTRFTELFEVIKAYAQGGYFHQDKALQIIAGTYVFMFER
                     EDMPDARPVVDGILEQYDYVFTTLERGNLDPLSVDAVVRVALYKDEYMEWGINRLGRI
                     LEALHRRSRADEAYADFIEDSAVVIRGLENIVAGSALEEIVEGNGA"
     gene            complement(1575..>3762)
                     /gene="uvrA"
                     /locus_tag="AC16CM_15970"
     CDS             complement(1575..>3762)
                     /gene="uvrA"
                     /locus_tag="AC16CM_15970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3141379.1"
                     /translation="DSVETALKLAEGLVQVETVERDGEGESMLFSESFTCTNCGASIA
                     EIQPRTFSFNSPHGACDRCDGLGSRLEIDPALVVPNEDLSVNEGALVPWANSQTEYHA
                     SVLGALAEKYGIDMNVPWRDMPGEQKDLILFGTDEERIYVSYRNRYNRRRQYMTRFDG
                     VVGNLERRYAETDSEYRREKIEEFMSHVPCPKCKGARLRPEALAVTVGGKNVSEFTAL
                     SVTDAQRFFDDVEFTTREWIIGERVVKEIRERLGFLVDVGLGYLTLSRSAGTLSGGEA
                     QRIRLASQVGSGLVGVLYVLDEPSIGLHQRDNRRLLTTLEKLRDLGNTLVVVEHDEET
                     IREADYVIDVGPGAGVHGGEIVAIGGIEDIEAEERSVTGDFLSGRRRIEPPEERREPT
                     GAVTLLGATEHNLKGVDVEFPLGVFTCITGVSGSGKSTLVNEILYKALANAVSRGKHR
                     PGRHAGIRGVEDVDKVIDIDQSPIGRTPRSNPATYTKVFDHIRQLFASTPEAKIRGYK
                     PGRFSFNVKGGRCEACRGDGQIRIEMHFLPDVYVPCEVCKGRRYNNETLRATYKGKSI
                     SEVLDMTVEEACEFFAPVPAISRRMDTLRDVGLGYVRLGQPATTLSGGEAQRVKLASE
                     LGKRATGKTVYILDEPTTGLHFADVEKLLSILHRLVDAGNTVIVIEHNLDVIRSADHV
                     IDLGPEGGDAGGEIVATGTPEDVANVDGSHTGRFLRGLMPEVVATG"
BASE COUNT          638 a         1231 c         1215 g          678 t
ORIGIN      
        1 cgttcacgac gctacagacc ctacatcctg gggtcattgt agaggttagt gctgaacgat
       61 gcttgaaagg tagggctcgc cgagatggcg gctactggcc cgccgccggc gaagctgatg
      121 agggagcctc tttctggctc gcggcgggcg aggcagagcc gccgctgtca gccccctgtc
      181 cggcaccggg ggaggcggaa ccgttccctc caggctcggg cgaagcggcg ggatcaggag
      241 atgccgaggg cacggctcca ccgccagggt tgccgacgac gggctggtca ccaccgccga
      301 aaggattacc accgagaggg ttaccgccat tctgacgcac acggtcaacc aggctagggg
      361 tttcggtccc ggccgcggaa ccgcccgaga acgaggagtt ggcgcccgtg ccaccataac
      421 ccatggggtg gatgcccggg gtggcaccgt aggggcattc ggaccacagg ccgcaggaga
      481 ggctgttacc gatcaccttc tccgttcccc acactaaccc catgcagagc aggaaggcca
      541 cgacggcggc cagaaaccct ttcatcagga ttgagcgccg tcggagggtc ggcaggttgg
      601 agaaccaccc gagggcggca cgcatcctgc ccgcgaagtt gtccctgagg tcggggcgtc
      661 ccggcatggt ggccccgccc tgctgctccc gccgcgtgcg cagcttcttc ggcatccccc
      721 gcacgtggcc cgagagcgtc tccaggtacg cgcgcaggat cgacgtcaag cccgtgacga
      781 gcatggccgt aagggcggcg cccagcagcg tgccggccac cccgaacttg gaggtcacca
      841 gggaggctcc cgtggccgcg atgccggccg acatgatctg gggcgtattg actaccggtg
      901 ttccactatt cgcactcacg gctgttgaat ctatcagggg gccggcgcgg tttcaaacgg
      961 tggcaggtaa atattcggta acagtttcga tcatcttccc gtagcgtcgg agcgcatccg
     1021 gcggttagaa tggcccgcag gcaaaaactg gaggagatca agcatgacag agcccgacac
     1081 cacgaggttc acggagctct tcgaggtgat aaaggcctac gcccagggcg gctattttca
     1141 ccaggacaag gcgctccaga tcatcgcagg aacctacgtg ttcatgttcg agagagagga
     1201 catgcccgac gcacgccccg tggtcgacgg catcctggaa cagtacgatt acgtgttcac
     1261 caccctggag cgtggcaacc tagatcccct gagcgtggac gccgtggtgc gggttgccct
     1321 ctacaaagac gagtacatgg aatggggcat caaccgcctg ggacgcatcc tggaggccct
     1381 ccaccgccgc tcccgcgccg acgaggccta cgccgacttt atcgaggact ccgcggtcgt
     1441 gatccggggc ctcgaaaaca tcgtcgccgg ttccgccctg gaagagatcg tcgagggaaa
     1501 cggcgcctga cccggaacgt ccgtcggccc gcggaccggg cggagttcga aacccgcccc
     1561 acatcgagtt tgtgctatcc cgtggccacg acctcgggca ttaggccgcg caggaagcgg
     1621 ccggtgtggg atccgtcgac gtttgcgacg tcttcggggg tgcctgtcgc gacgatctcg
     1681 ccgccggcgt caccaccctc cgggccgagg tcgatgacgt ggtcggcgga acgtatcacg
     1741 tcgaggttgt gctcgatgac tatgacggtg ttgccggcat cgacgagcct gtgcaggatg
     1801 gagagcagct tctccacgtc ggcgaagtgt aggcccgtcg ttggctcatc caggatgtag
     1861 acggtcttgc ccgtcgcccg cttgccgagc tcgctggcca gtttgaccct ctgggcctcg
     1921 ccaccggaga gcgtggtcgc gggctggccc agcctgacgt agccgagtcc gacgtccctc
     1981 aaggtgtcca tgcggcgtga gatggcaggc actggggcga agaactcgca ggcctcctcc
     2041 acggtcatgt cgagcacctc ggagatcgac ttgcccttgt aggtggcacg cagagtctcg
     2101 ttgttgtagc ggcggccttt gcagacctcg caggggacgt acacgtcggg aaggaagtgc
     2161 atctcgatgc ggatctggcc gtcgccccgg cacgcctcgc agcggccgcc cttcacgttg
     2221 aacgagaatc gtcccggctt gtagccccgg atcttggcct ccggcgtcga ggcgaagagc
     2281 tgccttatgt ggtcgaagac ctttgtgtag gtggcggggt tggagcgtgg cgtcctgcct
     2341 atcgggctct ggtctatatc tatgaccttg tccacatcct ccacgcccct gatgccggcg
     2401 tgccgcccgg gacgatgttt gccgcggctg acggcgttgg ccagcgcttt gtacaggatc
     2461 tcgtttacga gcgtgctctt gcccgagcca gacacaccgg tgatgcatgt gaacaccccg
     2521 agcgggaact ccacgtccac acccttgagg ttgtgctccg tagccccgag caacgtcacg
     2581 gcccccgtcg gctcgcgacg ttcctcgggt ggctctatgc gcctgcgacc agagaggaag
     2641 tcgcccgtca cggaacgctc ctcggcctct atgtcctcga taccgccgat ggcgacgatc
     2701 tcgccgccgt gcaccccggc ccccggcccc acgtcgatga cgtagtccgc ctcgcgtatg
     2761 gtctcctcgt cgtgctccac gaccacgagc gtgttgccga gatcgcgcag tttttcgagg
     2821 gtggtcagca gccgcctgtt gtcccgctgg tgtagcccga tggagggctc gtccagaacg
     2881 tacagcacgc cgacgagccc gctcccgacc tgagaggcga gccggatcct ctgcgcttca
     2941 ccccccgaga gggttcctgc ggagcggctg agggtaaggt acccaagccc cacgtcgacg
     3001 aggaaaccaa ggcgctcgcg gatctccttc accacacgct cgccgatgat ccactcccgc
     3061 gtggtgaact cgacgtcgtc gaagaagcgc tgcgcgtccg ttacggagag cgccgtgaac
     3121 tcggagacgt tcttgccccc gacggtaacg gccagcgcct cgggcctgag cctggcgccc
     3181 ttgcacttcg ggcagggaac gtggctcatg aactcttcta tcttctcccg cctgtactcc
     3241 gaatcggtct cggcgtagcg gcgctccagg ttgccgacga cgccgtcgaa gcgggtcatg
     3301 tactgccggc gacggttgta gcggtttcgg taggagacgt agatgcgctc ctcgtccgtg
     3361 ccgaacagga tgaggtcctt ctgctcccct ggcatgtcgc gccagggaac gttcatgtcg
     3421 ataccgtact tctcggcaag cgcaccgagc acgctggcgt ggtactcggt ctgcgagttg
     3481 gcccacggca ccagggcccc ctcgtttacg ctcaggtcct cgttcggaac gacgagcgcc
     3541 gggtctatct caagcctgct tccgaggcca tcgcagcgat cgcaggcccc atgcgggctg
     3601 ttgaacgaga acgtgcgcgg ctgtatctcg gcgatcgaag cgccgcagtt cgtgcaagta
     3661 aagctctcgg agaagagcat cgactcgccc tcgccgtctc tttcgacggt ttcgacctgg
     3721 actagcccct cggccagctt cagggccgtc tccacggagt cc
//
LOCUS       DBNGVD010000595         1572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3809320, whole genome shotgun sequence.
ACCESSION   DBNGVD010000595 DBNGVD010000000
VERSION     DBNGVD010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3809320"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1529)
                     /gene="gltX"
                     /locus_tag="AC16CM_15975"
     CDS             complement(51..1529)
                     /gene="gltX"
                     /locus_tag="AC16CM_15975"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3141380.1"
                     /translation="MDNHDKIRVRYAPSPTGRLHVGGVRTALFNWLFARKNGGTFVLR
                     IEDTDLERSTEESVEQLAGSLRWIGLDWDEGPGAGGDHPPYRQTERFDLYREAARRLL
                     DSGAAYYDFATPEELAEFRERARAEGRQPIYTGGDYRGMDPEEARRRIEAGEPHTVRF
                     RTPREGQTVVEDMIRGPVTFENANLEDFVLMKSSDTPTYNFAAAYDDAEMEISHVIRG
                     DGHLSNTPRQILIHQALGNELPAFAHVPQVLGADRKKLSKRHGAASVEDFAEMGYLPE
                     ALFNYLALLGAGYAADEEIFAPEELAERFRIDRVSGNPAILDEKKLRAVNSVYLRRKS
                     PAELADLAAPMLAEGGVIGTEDLDRSHLEEIMDLLKERIALTTEIPDSVGYFYGGTLD
                     YDESEFEKQFGKPFVRENFPELVERLEALPEWTEEAIEETVRGLAAEKEKGARHLIHP
                     LRFAVTGRTVSAGLFETMQLLGRDRSLLRAGDVVEKMERLPA"
BASE COUNT          239 a          519 c          509 g          305 t
ORIGIN      
        1 gattctaccc gacggtcgct acttcgtcaa aaggacggac gatgcgaggg tcaggcgggc
       61 aggcgctcca tcttctcgac cacgtctcca gcccggagca ggctcctatc ccgtcccaga
      121 agttgcatgg tctcgaagag gcctgcgctg acggtacgcc cggttacggc gaagcgcagg
      181 gggtgaatca ggtggcgtgc ccccttctcc ttctccgcgg cgaggccccg caccgtctct
      241 tcgatcgcct cctccgtcca ctccggaagt gcttcgaggc gttcgacgag ctcgggaaaa
      301 ttctccctga cgaaaggctt gccgaattgc ttctcgaact cggattcatc gtaatcaagg
      361 gtgccgccgt agaagtagcc cacggagtcg gggatctcgg tggtaagggc tatgcgctcc
      421 ttgaggaggt ccatgatctc ctcgaggtgg gacctgtcca ggtcttctgt cccgatcacc
      481 ccgccttccg cgagcatggg ggccgcgagg tctgcgagct ccgctgggct cttgcgcctg
      541 aggtagacgg agttgaccgc ccttagcttc ttctcgtcca ggatcgcggg gttgccactg
      601 acgcggtcga tgcggaagcg ctcggccagc tcctcgggtg cgaagatctc ctcgtcggcg
      661 gcgtagcccg cgccgaggag ggccaggtag ttgaagagcg cctcaggcag gtatcccatc
      721 tccgcgaaat cctcgacgct cgccgcgccg tgacgcttcg acaacttctt ccggtctgcg
      781 ccgagcacct gcggtacgtg ggcgaaggcc ggaagctcgt tgccgagggc ctggtggatc
      841 aggatctgac gcggcgtgtt ggataggtgg ccgtcgcccc tgataacgtg gcttatctcc
      901 atctcagcgt cgtcgtaggc ggcggcgaag ttgtaggtcg gggtgtccga agacttcatg
      961 agcacgaagt cttcgaggtt ggcgttctcg aaggttacgg ggccccggat catgtcctcc
     1021 accaccgtct gcccctcgcg cggggtcctg aaacggacgg tgtggggctc gccggcctct
     1081 atccgccttc tcgcctcctc ggggtccatc ccgcggtaat cgccgccggt gtagattggc
     1141 tgccttccct cggcccgcgc ccgctcccga aactcggcca gctcctcggg cgtggcgaaa
     1201 tcgtagtagg cggcgccgga gtcgaggagc ctcctggcgg cctcgcggta gaggtcgaag
     1261 cgctcggtct ggcggtaggg gggatggtcg cccccggccc ccgggccctc gtcccagtcc
     1321 aggccgatcc accgcagaga acccgctagc tgctcgaccg actcctccgt cgagcgctcc
     1381 aggtccgtgt cctctatgcg cagcacgaag gtgccgccgt tcttgcgcgc gaagagccag
     1441 ttgaagagcg ccgtgcgtac gccgccgacg tgcagcctcc ccgtcgggct cggcgcgtac
     1501 ctgaccctga tcttatcgtg gttatccatg gggtaaacct aacacaccac cccttgcgag
     1561 aacagcttgg ga
//
LOCUS       DBNGVD010000596         9210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_350820, whole genome shotgun sequence.
ACCESSION   DBNGVD010000596 DBNGVD010000000
VERSION     DBNGVD010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_350820"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="AC16CM_15980"
     CDS             <1..93
                     /locus_tag="AC16CM_15980"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HIR3141381.1"
                     /translation="HVFEDGPAPTGQRYCINSAALRFDPEAASE"
     gene            272..1654
                     /locus_tag="AC16CM_15985"
     CDS             272..1654
                     /locus_tag="AC16CM_15985"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR3141382.1"
                     /translation="MATRQIQEERVQNLNEKDVLDGDYVLYWMQSSQRAEQNHALEYA
                     VQRANELGQRLLVVFGLTDDYPEANLRHYAFMLEGLRDAQESLKERGIKMVVRQGSPD
                     EVALEAGEDASLIVTDRGYMRPQKRWRERVAGEAGCLVTQVESDVVVPVELASVKQEH
                     AARTLRPRIGEHLEDFLVALTPTGVQKQSTNMPAGGLDLSDIDGVLDGMDLDRSVGAL
                     GHLYTGGNSGAKEMFRRFLKNSFDAYVEHRNQPQTDDVSHMSKYLHFGHVSPIWLALE
                     ARGAKTEKDNIESFVEELVVRRELSMNFVFYNDDYDSFSNLPGWARETLREHKDDERE
                     YVYTTKQLENAETHDEYWNAAMKEMVHTGYMHNYMRMYWGKKILEWSNTPEHAYRTTL
                     YLNNKYFLDGRDPNSFANVAWVFGQHDRGWTEREVFGKVRFMSAGGLERKTKPDQYVE
                     KVEKRIEAPG"
     gene            1745..2338
                     /locus_tag="AC16CM_15990"
     CDS             1745..2338
                     /locus_tag="AC16CM_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141383.1"
                     /translation="MVDAGEYETGRPISSPLSTTREVPFSPRRFFNGLPPDGPPREPV
                     LYFLICSTLTAVINTAATVAFFAVPAGIALATGSVEAGLLLRLVAVFVAASLVVVPAS
                     FVVGFLLSVPLQHGLILRVAGRNSVGAPVAVAWIPLAGFLAVFYCLYLHATGLRRVHR
                     ISTGRSFVATLLLTVFLPALATALIIYEYRVIQRLMG"
     gene            complement(2397..2963)
                     /locus_tag="AC16CM_15995"
     CDS             complement(2397..2963)
                     /locus_tag="AC16CM_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HIR3141384.1"
                     /translation="MSRTPIHLETPLSMEDIEPLKSGDVVLLSGELYTARDAAHARMR
                     EAIEAGEPLPFDLEGQVVYFTGPAPARPGQALGPAGPTTASRMDSHSPMLVERGLRGM
                     VGKGSRAEAVREAMRRHGCVYFGAVEGTAALLADRVKEAEVIAYEDLDAEAIRRLVVE
                     DFPVVVVNDLHGGDLYTEGRERWRRRKP"
     gene            complement(2960..3802)
                     /locus_tag="AC16CM_16000"
     CDS             complement(2960..3802)
                     /locus_tag="AC16CM_16000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR3141385.1"
                     /translation="MREIEARSVIEAVRDLCIEANTRLPGGHLAALRRALEEEESELG
                     REVIERLLQNAEVARERCIAFCQDTGYAVFFVEMGSEMRISGGEIGAAIDEGVRRGYD
                     EGYLRKSIVRSPVDRTNTGDNTPAIVYYDPVPGDGLKLTMLVKGAGCDNMSALRMLTP
                     AEGIPAMKEFVVETIERAGPNASPPLTVGVGIGGPFERAALLAKKALTRPSGEPNPDP
                     EIAGIERELLEEINATGIGPAGYGGTQTALAVHVESFPTHIAAFPVAVNLDCHSHRTA
                     STEL"
     gene            4025..5200
                     /locus_tag="AC16CM_16005"
     CDS             4025..5200
                     /locus_tag="AC16CM_16005"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3141386.1"
                     /translation="MTTQEKSRQGFWQSLFGRDGRQETREQVPEAVVEPTVQETGPEL
                     KIAPNDPIVAYFASASGVVEIEKLDLDSPALRAMKEADIKLVVPLVSQGELIGLLNLG
                     PRLSQQEYSADDRKLLNDLATQTAPAVQVAQLVRQQQQQAQERERIEQELRVARLIQQ
                     TLLPKSLPNLPGYEVAAYYQPAREVGGDFYDFLELEDGRFGLVVGDVTDKGVPAALVM
                     ATTRTLLRASAQRLYSPGEVLRRVNEVIVQDIPPNMFITCLYAILDEESGHLIYANAG
                     HDLPYRRKAAAGGAEELRATGMPLGLLPGMEYEEKEIVLERGDSVLFYSDGLVEAHDP
                     RREMFGFPRLQGLVAGHRTGGEAMVNYLLSELSRFTGEEWEQEDDITLVTLERSETA"
     gene            5197..5670
                     /locus_tag="AC16CM_16010"
     CDS             5197..5670
                     /locus_tag="AC16CM_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3141387.1"
                     /translation="MTDPKPRTDADFGRTLADFSVPSEPGNERLAMETVAKTAQGFGF
                     SGENLERLKTAVAEATMNAMEHGNNYSPELPVELQVLATDYDFLVRITDRGSGPPPTD
                     AGDTPDIEAKLEGLQKARGWGLFLIKSMVDEMNVTGDEGHHTVELVVHLGAEGGK"
     gene            5670..6026
                     /locus_tag="AC16CM_16015"
     CDS             5670..6026
                     /locus_tag="AC16CM_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3141388.1"
                     /translation="MEVRHLSVEVRLEGDIAVLDLTGEINGFAEEALSAAYAEAEAMD
                     PSTVLLNFEKVDYINSTGIALIVGLLAKARASRRGLLACNLSEHYVEIFNITRLSDFM
                     SVFPDEKTAVGETSGA"
     gene            6028..6474
                     /locus_tag="AC16CM_16020"
     CDS             6028..6474
                     /locus_tag="AC16CM_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3141389.1"
                     /translation="MDEPLDGSLQYLRGEYEQTREIYRKRRNEMPEAQVKMHVRRAGD
                     FASIIDVEGELTAFAEGVLMDAYNQASDGQARAIILNFEELEYMNSSGIGLLVTLLIR
                     INRENQRLMTYGLSDHYKSIFQITRLDDAIAIHDSEEEAVRAANSA"
     gene            6490..7272
                     /locus_tag="AC16CM_16025"
     CDS             6490..7272
                     /locus_tag="AC16CM_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141390.1"
                     /translation="MTEQTPQEEPMQEKEPGREPRRAPRDAAYWATYAETLKVTGVAE
                     GATNINVEGRRAVGPLQGFGKLWQKTYRVALKGAEATPTEVIKTWKDNYQEFWPEGNR
                     FYAPLAGIAPGEVALISGSMPGGVKLSTGVMVLYADDESFSLMTPEGHPFSGWITFSS
                     FEEEDTTIAQAQVLMRANDPLYEMGLRMGGHKMEDQIWQKTLTNLATHFGVHEPVETN
                     LVCVDPKLQWSQYRNIWHNAGIRSALHMMAAPFRSRGKGAGN"
     gene            7293..8060
                     /locus_tag="AC16CM_16030"
     CDS             7293..8060
                     /locus_tag="AC16CM_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141391.1"
                     /translation="MSESARGPEEQPKARRDPRDAGSWAVGHNILRVSDVPEGAVNLN
                     VDGRRVIGALQGFGQLWQKTYTLRLAGADVTPEEVVALWKREFPDFHPPESRFYPSLD
                     GVEPGEIMLINATVQGMPVYTGVMVLYSDDVSFTVMTAEGLPEAGWNTFSAYEENGVT
                     VAQIQSLARASDPIYEVGFRLFGSTAQEKIWTHVLSGLAGRFGVRGDVGLEKACVDPK
                     LQWYRVGNIWHNAGLRSVLYTLVAQARVVSGLFRRRS"
     gene            8079..>9210
                     /locus_tag="AC16CM_16035"
     CDS             8079..>9210
                     /locus_tag="AC16CM_16035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3141392.1"
                     /translation="MSERATYDAVVVGSGPNGLAAAVELARNGKRVAVLEGKSTIGGG
                     ARSAEITLPGFVHDLGSAIHPLGYASPFFRSLPLEEHGLEWVHPDAPLAHPFDGGEAA
                     VLERSLEATAAGLGPDADAYRRLIEPGTADASRIAGFFVGEPSLIRHPLALAFSGIKA
                     LGAAESLAERVFEGKKARGLFAGNAAHSFLPLDQPPSALFGLLLGTLGHAFGWPFPRG
                     GSQSLADALASYLKSMGGEIFTNAPVSTVDDVPRTRAVLFDVTPRQLHDIAGEHFTDD
                     YNRALKRYRYGPGVFKVDFALDGPIPWEAEGCRRGGTVHLGGTLEEVAAGEATVARGE
                     HPERPFILLAQQSLFDDTRAPEGKHTVWAYCHVPNGSTFDMTE"
BASE COUNT         1714 a         2969 c         3006 g         1521 t
ORIGIN      
        1 cacgtattcg aggacggacc ggccccgacc ggccagcgct attgcataaa ctcggccgcc
       61 ctccgcttcg atcccgaagc cgcttcggaa taagagtttt cagctttcag cggtcagctt
      121 tgatctcggc tgatagctaa ccgggctttg aaaagcgccg atatctctgc atttcgctgt
      181 gccggcgagg ttttgtgttc caggatccac ttttcctgat gtctttcctc taatcttgct
      241 tcaggcgaga aattctgggg gtagaaggca cttggctacg cggcagatac aagaagagcg
      301 tgtgcagaac ctgaacgaga aggacgtgtt ggacggcgac tatgtgctgt actggatgca
      361 gtcttcccag cgcgccgagc agaaccacgc cctcgagtac gccgtacagc gggcgaacga
      421 gctggggcag cgcctgctcg tggtcttcgg cctcaccgac gactatcccg aggcaaacct
      481 gcgccactac gcgttcatgc tcgaaggctt gagagacgcc caggaatctc tcaaggagcg
      541 tggcatcaag atggtggttc gtcagggctc tccggacgag gtggcgctag aggcgggcga
      601 ggacgcctcg ctcatcgtca cggaccgcgg ctacatgcgc ccacagaaga ggtggcggga
      661 gcgggtcgcg ggggaggcag gatgcctcgt gacacaggtc gagtccgacg tggttgtgcc
      721 cgtcgagctc gcctccgtca agcaggagca cgcggccagg acgctcaggc ccaggatcgg
      781 cgagcacctg gaggatttcc tggtggcgct cacaccgacc ggggtccaga aacagtccac
      841 caacatgccg gctgggggcc tggatctctc ggacatcgac ggcgtacttg acgggatgga
      901 tctggaccgg agcgtcggcg ccctgggcca cctctatacc ggtgggaact ctggtgcgaa
      961 ggagatgttt cgtcgtttcc tgaagaacag cttcgacgcc tacgtcgagc accgcaacca
     1021 gccgcagacc gacgacgtct cgcacatgag caaatacctg cacttcgggc acgtctcccc
     1081 gatctggctc gccctggaag ccaggggggc gaagacggag aaagacaaca tcgagtcgtt
     1141 cgtcgaggaa cttgtcgtga ggcgcgagct gtccatgaac ttcgtgttct acaacgatga
     1201 ctacgactcg ttctccaacc ttcccggctg ggcgagggag acgcttcggg agcacaagga
     1261 cgacgagcgg gaatacgtct acaccacaaa gcagctggag aacgccgaga cgcacgacga
     1321 gtactggaac gcggccatga aggagatggt tcacaccggc tacatgcaca actacatgcg
     1381 catgtactgg gggaagaaga tcctggagtg gtccaacacc ccggagcatg cctacaggac
     1441 caccctctac ctcaacaaca agtactttct ggacggccga gatccaaact ccttcgctaa
     1501 cgtggcctgg gtgttcggcc agcacgaccg cggctggacc gagcgcgagg tcttcggcaa
     1561 ggttcgtttc atgtccgccg gtggcctgga gcgcaagacc aagcccgacc agtacgtcga
     1621 gaaggtcgag aagaggatag aggccccggg ctgaccaacg ggcctatact tccgcccgga
     1681 agactagaag ggcggaggca gggcttcggg gtcgcgagag gtaaaggaaa gcgccgtccc
     1741 ccagatggtg gacgcgggcg aatacgagac gggtaggccc atatcgagcc cgctctcaac
     1801 cacacgcgag gtgcccttct caccgcggcg tttctttaac ggtctgccgc cagatgggcc
     1861 gcccagagag cccgtgctct atttcctgat ctgctcgact ctaacggcgg tcatcaacac
     1921 ggccgctacg gtagctttct tcgccgtacc cgcaggcatc gccctggcaa caggctcggt
     1981 cgaagccgga cttctcctcc gcctcgtggc ggttttcgta gctgcatcgc tagtggtcgt
     2041 gccggcgtcc ttcgtggttg gcttcctcct ctcggtgccg ctacaacacg gccttatcct
     2101 tcgcgtggcc ggcagaaact cggtgggggc tcccgtggcc gtggcctgga taccgctggc
     2161 cggctttctg gccgtcttct actgcctcta cctgcacgcg accggcctga ggcgggtaca
     2221 ccggatctcg accggcagat ccttcgtggc caccctcctg ctcacggtat tcctgccagc
     2281 cctggccaca gccctcatca tctacgaata ccgcgttatc cagagactca tgggatagcc
     2341 gatccgaagc gagtagaaag ctgacagctg agagctagaa accgagagct aacaacctac
     2401 ggcttccgcc tacgccagcg ttcgcggcct tccgtgtata ggtcgccgcc gtggaggtcg
     2461 ttcaccacga cgaccggaaa gtcctcgacg acgaggcgac ggatagcctc cgcgtcgagg
     2521 tcctcgtagg cgatgacctc ggcctctttc acgcggtcgg cgaggagtgc ggccgtaccc
     2581 tcgacggccc cgaagtacac gcagccgtgc ctccgcatcg cctcgcggac ggcctccgcg
     2641 cgggagcctt tgcccaccat cccccgaagc ccccgctcca ccagcatcgg cgagtgcgag
     2701 tccatgcgcg acgccgtcgt gggcccggcg ggcccgaggg cctgtccagg gcgtgcgggg
     2761 gctggcccgg taaagtagac gacctggcct tcgaggtcga aaggaagcgg ttctcccgcc
     2821 tctatggcct ctctcatcct ggcgtgggcc gcgtcgcgcg ccgtgtagag ctcgccgctc
     2881 aggagcacca cgtcgccgct cttcaggggt tcgatgtcct ccatcgagag cggcgtttcc
     2941 agatggatcg gcgtgcggct cacagctcgg tggaggcggt acggtgggaa tggcagtcga
     3001 ggttcacggc cacgggaaag gcggctatgt gggtcgggaa gctctccacg tggacggcga
     3061 gtgccgtctg cgtcccgccg tagccggccg gcccgatacc ggtggcgttg atctcctcca
     3121 gcagctcgcg ttcgatcccg gcgatttccg ggtcagggtt cggctcaccg gacgggcggg
     3181 taagcgcctt cttggccagc agggcggccc tctcgaaggg gccaccgata ccgacgccca
     3241 ccgtgagcgg tgggctggcg tttgggccgg ccctctctat cgtctcgacg acgaactcct
     3301 tcatggcggg gatgccctcc gccggggtaa gcatcctcag ggcgctcatg ttgtcgcagc
     3361 cggcgccctt gaccagcatc gtcaacttca ggccgtcgcc tgggacgggg tcgtagtaga
     3421 cgatggccgg ggtgttgtcg cccgtgttcg tcctgtctac cgggctcctg acgatggact
     3481 ttctcaggta gccttcgtcg tagcccctgc gaacgccctc gtcgatggcg gccccgatct
     3541 ctccgcccga gatcctcatc tcgctgccca tctcgacgaa gaacacggcg tagcccgtgt
     3601 cctggcagaa ggcgatgcac cgctcgcggg cgacctcggc gttctgcagg aggcgttcga
     3661 tcacctcgcg ccccagctcc gattcttcct cttcgagagc ccggcgcagg gctgccaggt
     3721 gcccccctgg caggcgtgtg ttcgcctcga tgcacagatc tcgcaccgct tcgataaccg
     3781 acctggcctc tatctcgcgc atcgctctct cgcttccccg gccgcctcgc aagaagaagg
     3841 ttaacacccc cggcggccga ggttctccgc tctggcccag cttaccgggg ctctcgcctc
     3901 tagatccaaa agatgaagac gtgagacaat caggccggaa caatgctggc gaaccagatt
     3961 tcgtaacgct tttcgtaacg tctgggagac gttggacgct tagaatcgag atcgaggagg
     4021 tcagatgacg acacaggaga aaagcaggca agggttctgg cagagtctct tcggaagaga
     4081 cggaaggcag gagacccgcg agcaagtccc cgaggccgta gtcgagccca cggtccagga
     4141 gaccgggccc gagctcaaga tagcgccaaa cgaccccatc gtcgcctact tcgcgagcgc
     4201 ttcgggggtt gtcgagatcg agaagctcga cctcgactcg cccgccctca gggccatgaa
     4261 ggaagcagac atcaagttgg tcgttcctct cgtgagccag ggggagttga tcgggctcct
     4321 caacctcggt ccccgcctct cccagcagga gtattcggct gacgacagga agctcctgaa
     4381 cgaccttgcc acccagaccg cgcctgcggt gcaggtggcg cagctcgtcc gtcagcagca
     4441 gcaacaggcc caggagcggg agcgcatcga gcaggagctc agggtggcac gcctgatcca
     4501 acagacgctc ctgcccaagt cgctgccaaa cctgcccggt tacgaggtgg cggcctacta
     4561 ccagcccgcg cgcgaggtgg gcggcgactt ctacgatttc ctggagctcg aagacggccg
     4621 gttcggcctc gtcgtcggcg acgttacgga caagggcgtg cccgccgcgc tggtcatggc
     4681 gacgacccgc acgctgctcc gcgcttcggc ccagcgcctc tactcgcccg gcgaggttct
     4741 ccggcgggtc aacgaggtga tcgtccagga cataccgccc aacatgttta tcacctgcct
     4801 ctacgcgatc ctcgacgagg agtccggcca cctcatctac gccaacgccg gccacgacct
     4861 tccttacagg cgcaaggccg cggcgggcgg cgcggaggag ctcagggcca cgggcatgcc
     4921 gctcgggctg cttcccggca tggagtacga ggagaaggag atcgtgctgg agagggggga
     4981 cagcgtgctg ttctacagcg acggattggt cgaggcccac gacccgcgca gggagatgtt
     5041 cggcttcccg cgcttgcagg ggctcgtggc cgggcaccgc acgggcgggg aggccatggt
     5101 caactacttg ctctccgagt tgtcgcgctt caccggagaa gagtgggagc aggaggacga
     5161 cataacgctc gtcaccctcg agagatcgga gacggcgtga ccgatccaaa accgcggacg
     5221 gatgccgact tcggccggac cctggccgac ttctcggtcc cgagcgagcc gggcaacgag
     5281 cggctggcaa tggagacggt cgccaagacg gcgcaggggt tcggtttctc cggggagaac
     5341 ctggagcggc tcaagaccgc cgtggcagag gcgacgatga acgcgatgga gcacggcaac
     5401 aactacagtc cggagctgcc cgtggagttg caggtcctcg caacggacta cgacttcctg
     5461 gttcgcataa ccgacagggg gagcggccca ccgcctacgg atgccggcga tacgccggat
     5521 atcgaggcca agctggaggg cctccagaag gccaggggct ggggcctctt cctcatcaag
     5581 agcatggtgg acgagatgaa cgtaacaggc gacgaggggc accacaccgt ggagttagtc
     5641 gtccacctcg gagcggaagg aggtaagtga tggaggtacg ccacctgagc gtggaggtcc
     5701 gcctcgaagg ggacatcgcc gtgctcgacc ttacgggtga gatcaacggc ttcgccgagg
     5761 aggccctgag cgccgcctac gcagaggccg aggccatgga cccgagcacc gtgctcctca
     5821 acttcgagaa ggttgactac atcaactcga cgggcatagc cctgatagtg gggctgctcg
     5881 ccaaggcgcg cgcctcccgc cgcggcctgc tcgcctgcaa cctcagcgag cactacgtgg
     5941 agatcttcaa catcacgcgc ctctccgatt tcatgagcgt cttccccgat gagaagaccg
     6001 ccgtgggcga gacatcgggg gcgtgagatg gacgaaccac tcgacggctc gcttcaatac
     6061 ctgcgcggcg agtacgagca gacgagagag atataccgga aaaggaggaa cgagatgccc
     6121 gaggcccagg tgaagatgca cgtcaggcgg gcgggtgatt tcgccagcat catagatgtg
     6181 gaaggggagc tgaccgcgtt cgcggaaggc gtgctgatgg acgcctacaa ccaggcgagc
     6241 gacgggcagg cccgggccat catcctgaac ttcgaagaac tcgagtacat gaacagcagc
     6301 ggcatcgggt tgctcgtcac gctgctgatc aggatcaacc gcgagaacca gcggctgatg
     6361 acctacggcc tcagcgacca ctacaagagc atcttccaga tcacacgcct cgacgacgcc
     6421 atagcgatcc acgactccga ggaggaggcc gtgagggcgg cgaactcggc ctgagcaagg
     6481 gagagagtga tgacggaaca gacaccgcag gaagagccga tgcaggagaa agagcccggt
     6541 cgcgagccac gccgtgcgcc ccgcgatgca gcctactggg cgacctacgc ggagacgctc
     6601 aaggtaaccg gcgtggcgga gggcgcgacg aacatcaacg tggagggccg cagggccgtc
     6661 gggcccctgc agggcttcgg caagctgtgg cagaagacct accgcgtggc cctcaaaggt
     6721 gccgaggcga cgccgaccga ggtcatcaag acctggaagg ataactacca ggagttctgg
     6781 ccggagggta accgcttcta cgcaccgctc gccggtatcg ccccggggga ggtcgccctc
     6841 ataagcggct ccatgccggg tggcgtcaag ctctccaccg gggtcatggt gctctacgca
     6901 gacgacgagt ccttctccct catgacccca gaggggcatc ctttctccgg ttggatcacg
     6961 ttcagttcct tcgaggagga ggacaccacg atcgcccagg cccaggtcct gatgcgggcc
     7021 aacgaccccc tctacgagat gggcctgcgc atgggcggcc acaagatgga ggaccagatc
     7081 tggcagaaga cgctgacgaa cttggccacg cacttcggcg tccacgaacc cgtcgagacg
     7141 aacctggtgt gcgtagaccc caagcttcag tggagccagt acaggaacat ctggcacaac
     7201 gccggcatac gctccgcact ccacatgatg gcggccccct tccgttcgcg tgggaaaggg
     7261 gcggggaact gagcgaccag gggaggtggg agatgagcga gtccgccaga gggccggaag
     7321 agcagccgaa agccaggcgg gatccccgcg acgcggggtc ctgggctgtg gggcacaaca
     7381 tcctgcgggt ctccgacgtg cccgaaggcg ccgtcaacct caacgtcgac ggccggcgcg
     7441 tgatcggggc cctgcagggc ttcggccagc tctggcagaa gacctacacg ctccgcctcg
     7501 cgggggctga cgtcaccccc gaggaggtgg tggccctctg gaagcgcgag ttccccgact
     7561 tccacccgcc cgagagccgg ttctacccct ccctcgacgg ggtcgagccg ggtgagatca
     7621 tgcttatcaa cgccaccgtg caggggatgc ccgtgtacac cggggtcatg gtcctctact
     7681 ctgacgacgt ctccttcacg gtgatgacgg ccgagggcct gcccgaggct ggctggaaca
     7741 cgttctcggc ctacgaggag aacggcgtga cggtcgccca gatacaatcg ctcgcccggg
     7801 cgagcgaccc gatctacgag gtcggcttcc gcctgttcgg gtcaacggcc caggagaaga
     7861 tctggaccca cgttctctcc gggctggccg ggcgtttcgg ggtgcgggga gacgtgggcc
     7921 tggagaaggc ctgcgtggac ccgaagctgc agtggtacag ggtcggaaac atctggcaca
     7981 acgccggcct gaggtcggtc ctgtacacgc tggtcgccca ggcccgcgtt gtgagcggat
     8041 tgtttcgccg tcggagctga agggtctaga atcaaggcat gagcgaacgc gccacatacg
     8101 atgccgtagt tgttggctcg ggcccgaacg ggctcgcggc cgccgtagag ctcgcccgca
     8161 acgggaaacg cgtggcggtt ctcgaaggca agagcaccat cggcgggggt gccaggtcgg
     8221 cggagataac gctccccggc ttcgtccacg acctcggctc tgccatccac cctctgggct
     8281 acgcctcgcc tttcttccgt tccctgccgc tggaagaaca cggcctcgaa tgggtacacc
     8341 ccgacgcccc gctcgcccac ccgttcgacg gcggcgaggc ggcggtgctg gagcgttccc
     8401 tggaggccac ggctgcaggg ctcggccccg acgcggatgc ctatcgccgc ctgatagagc
     8461 cgggcaccgc cgacgcgagc aggatcgctg gcttcttcgt gggggagccg agcctgatcc
     8521 gtcaccccct cgccctggcg ttctcgggca taaaggccct tggcgccgcc gaaagcctgg
     8581 ccgaacgtgt cttcgagggc aagaaggcca ggggcctctt cgcggggaac gcggcccact
     8641 ctttcttgcc gctagatcaa ccaccgagcg cgctgttcgg gctgctcctt ggaaccctgg
     8701 gccacgcctt cggatggccc ttcccccggg gcggctcgca gtcgctcgcc gacgcgctcg
     8761 cctcctacct gaaatccatg ggcggtgaga tctttaccaa cgccccggta agcacggtcg
     8821 acgacgtgcc gcggacccgc gctgtgctct tcgacgtgac gccgcgccag ctccacgata
     8881 tcgccggaga acacttcacc gatgactaca acagggcgct gaaacgctac cgttacgggc
     8941 ccggcgtctt caaggtggac ttcgccctcg acggtcctat cccctgggag gccgaagggt
     9001 gcaggcgcgg gggaacggtc cacctcgggg gcacgctgga ggaggtcgcg gcgggggagg
     9061 ccacggtcgc gagaggcgag catcccgaga ggcctttcat cctgctcgcc cagcagagcc
     9121 tcttcgacga cactcgcgcc cccgagggca agcacacggt gtgggcatat tgccacgtcc
     9181 ccaacggctc aaccttcgat atgacggaga
//
LOCUS       DBNGVD010000597         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_534003, whole genome shotgun sequence.
ACCESSION   DBNGVD010000597 DBNGVD010000000
VERSION     DBNGVD010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_534003"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="AC16CM_16040"
     CDS             complement(<1..2003)
                     /locus_tag="AC16CM_16040"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3141393.1"
                     /translation="MKRSKSADPAHDVLGYEREQLDAIFRPETVAVIGATDRPGSVGR
                     TIMWNLVSSPFGGTVFPVNSHRPNVLGIKAYPSISQVPARVDLAVIVAPAPAVPDIIG
                     ECVEAGVEGAIIISAGFRETGPEGVELEQRVLEEARKGRIRVVGPNCLGVMKPPSGFN
                     ATFAGAMARPGNVALLSQSGALLTAILDQSLMENVGFSALASVGSMMDVGWGDLIYYL
                     GDDPQTKSIVIYMESVGDARTFLSAAREVALTKPIIVIKAGRTEAAGKAAASHTGSLT
                     GSDEVLDAAFSRSGVLRVDEISELFEMAEVLGKQPRPKGPRLTVLTNAGGPAVLATDA
                     LIGGGGELAGISEETMEALNGFLPGPWSHGNPIDVLGDADPERYTETLRTAADDPEGD
                     GLLVILTPQDMTDPTATAERLAPYAQGTRKPVLASWMGGPAVAAGDSILNGAGIPTFD
                     YPDAAARAFTNMWKYTYNLRSIYETPEPAQDEGADRRRVEEVMDRVLRSGRTILTEFE
                     AKEVLAAYGIPTVETRVARSPEEAVDFADRIGYPVVLKLDSETITHKTDVGGVRLNLQ
                     NADEVRRAYEDMESSITENYSPADFKGAAVQEMVSLDGYELIVGSSLDPQFGPVLLFG
                     SRGSLVEVYRDRSLALPPLTTTLARRMMERTRIFEALGGVRGR"
BASE COUNT          361 a          796 c          754 g          428 t
ORIGIN      
        1 tccctgcccc tgacgccgcc cagggcctcg aagatccgtg tccgctccat catgcgcctc
       61 gcgagcgtgg tggtaagggg tgggagggcg agggatcggt ccctgtagac ctcgaccagc
      121 gagccgcggg agccgaagag cagtacgggc ccgaactgcg ggtcgaggct gctacccacg
      181 atcagctcgt acccgtcgag gctgaccatc tcctgcacgg ccgcgccttt gaaatccgcg
      241 ggagagtaat tctccgtgat cgaggactcc atatcttcgt aggcccgccg cacctcgtct
      301 gcgttctgca ggttcaggcg cacccctccc acgtcggtct tgtgcgtgat cgtctccgag
      361 tcgagcttga gcacgacggg atagccgatg cggtcggcga aatccacggc ctcctcgggg
      421 ctgcgggcca cccgcgtctc aaccgtcggg atgccgtagg cggccaggac ctccttggcc
      481 tcgaactcgg tgaggatggt tcggcccgat ctgaggactc gatccatgac ctcctccacc
      541 cgcctgcggt cggccccctc gtcctgggct ggttcgggcg tctcgtagat gctgcgcagg
      601 ttgtaggtgt acttccacat gttcgtgaaa gcgcgggccg ccgcgtcggg atagtcgaag
      661 gtcgggattc ccgcgccgtt caggatcgag tcgcccgcgg ccacggcggg gccgcccatc
      721 cagctcgcga gcacgggctt gcgggtgcct tgcgcgtagg gtgcaagcct ctcggccgta
      781 gccgtcgggt ccgtcatgtc ctggggcgtg agtatcacca gcaggccgtc gccctccggg
      841 tcgtccgccg ccgtccggag cgtctcggtg taccgctctg gatctgcgtc gccgagcacg
      901 tctatggggt tgccgtggct ccaggggccg ggaaggaaac cgttcagggc ctccatcgtc
      961 tcctcggaga tcccggccag ctcgccgccg ccccctataa gggcgtccgt ggcaagcacg
     1021 gcgggacctc cggcgttggt cagaaccgtc aggcgcgggc ccttcgggcg cggctgtttg
     1081 cccagcacct ccgccatctc gaagagctcg gagatctcgt ctacgcgcag gacgccgctg
     1141 cgggagaagg ccgcgtcgag cacctcgtcg gagccggtca gggagccggt gtgggaggct
     1201 gccgccttgc ccgcggcctc cgtgcgtccg gctttgatca ctattatggg cttggtgagg
     1261 gcgacctcgc gggccgcgga taggaaggtg cgggcgtcgc ccacggactc catgtagatc
     1321 acgatgctct tggtctgggg gtcgtcgccg aggtagtaga tgaggtcccc ccacccgacg
     1381 tccatcatcg acccgacgct ggcgagcgcc gagaagccca cgttctccat gaggctctgg
     1441 tccagaatgg ccgtgagcag ggccccgctc tggctcaaca gcgccacgtt gccgggacgg
     1501 gccatggcgc cggcgaaggt ggcgttgaag ccagacggcg gcttcatcac gccgaggcag
     1561 ttcggcccga ccacccgtat cctgcccttc ctggcctcct cgaggacccg ctgctcgagc
     1621 tccacgccct cgggccccgt ctccctgaaa ccggccgaga tgatgatggc gccctccaca
     1681 ccggcctcca cgcactcccc gattatgtcg ggcaccgcgg gcgctggtgc cacgatgacc
     1741 gcaaggtcca ccctcgctgg cacctgcgag atgctgggat aggccttgat gccaagcacg
     1801 ttcgggcggt gcgagttgac cggaaagacc gtacccccga acgggctgct caccaggttc
     1861 cacatgatgg tgcgccccac gctcccgggc cggtcggtgg cccctataac ggcaaccgtc
     1921 tcaggacgga aaatggcatc caactgctcc cgctcatatc ccagaacatc gtgggcggga
     1981 tcagcgctct tggaacgctt catctgtcgc tcccttcggt cgctcccggc tacaggcatc
     2041 aggctacagg tttcaggtct tcgcccgcgg tactcctggc cgaggtcctg atactatgtt
     2101 ccgaatgcac ctactttgta ccgctgcacg taagagaagg tagtgaaccc gagcactggc
     2161 tccatcagcg atacgttttc ctgatgcctg aaacctgtag cctgtaacct cgccccgctt
     2221 gtggattcac gttcctccgt attcccctcg acgcagcccg aggtaataca cgaaggcgtc
     2281 cacgtaggag cgtccgaggg cgcttacgaa cgtctcggtg ttgcgcatga aggcggaga
//
LOCUS       DBNGVD010000598         3419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3994457, whole genome shotgun sequence.
ACCESSION   DBNGVD010000598 DBNGVD010000000
VERSION     DBNGVD010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3994457"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1295)
                     /locus_tag="AC16CM_16045"
     CDS             complement(327..1295)
                     /locus_tag="AC16CM_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR3141394.1"
                     /translation="MNSLEHMRDAVFFEGPERGRRLSRFWILIVLASIIAAAGVVADS
                     TATVIGAMIVAPLMTPILGTMLSVLIADRANLTRSLFLVVSGAAVAVVIGYLLGLTSV
                     TGVVAETNSQVAGRVHPRLIDLLAALATGAVGSVALVRQDISDTLPGVAIAISLVPPL
                     SVVGLTFESGAPDQSLGALLLFLTNVAAILGSGTVVMAIYGVHRLAASPDSPGQRTVN
                     RRNAVIAIAAMIVAVVVPLTATSVSIARDSLREARVSDAARAFGKEVGWSTADITTRK
                     GRVVVLMEGPLPVPDPDPLESQLKERGVAPSEVRVELDPVRIVTFD"
     gene            complement(1546..1875)
                     /locus_tag="AC16CM_16050"
     CDS             complement(1546..1875)
                     /locus_tag="AC16CM_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141395.1"
                     /translation="MSEASSWAEMEHRLVQRSTEDDVFRQRLLSDPKAAVEQELGTRL
                     PEDVRVVALEETAQTIYLVLPNSSPLGESGELSDQELESVAGGWDAGTSGFTCTLEQT
                     RCGVECA"
     gene            2130..2465
                     /locus_tag="AC16CM_16055"
     CDS             2130..2465
                     /locus_tag="AC16CM_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3141396.1"
                     /translation="MRYMLLICEDPSAEAVPEDGLGAATETWVEEMDGRGVRKVGDRL
                     RPASDATTVRVRDEGVLLSDGPFAETREQIAGFDLVECEDLDEAVEVASRHPMARYGM
                     IEVRPFWEA"
     gene            2453..>3419
                     /locus_tag="AC16CM_16060"
     CDS             2453..>3419
                     /locus_tag="AC16CM_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3141397.1"
                     /translation="MGGVRQNVERAVARAHREEWGRIVATLIRTTGDWDLAEECAQEA
                     FARALETWPRDGVPRRPGAWLTTTARNRARDFLRRRAIGSARLGEVALLSGQDEPGSD
                     VTEGSGVQDDRLRLIFTCCHPALSLEARVALTLRTLAGLTTPEIARAFLVSEQTMAKR
                     LVRARAKIRNARIPYRVPRAHLLPERTGGVLAVLYLLFNEGYAATAGADLVRAGLSDE
                     AIRLSSTLARLMPDEPEALGLLALMLLQDARRAARTDGAGDLIPLDEQDRSLWDRAAI
                     AEGVRLLDAALRLGRPGPYQVQAAIAACHATAPDASSTDWDEITVL"
BASE COUNT          599 a         1148 c         1101 g          571 t
ORIGIN      
        1 tgcatgctcc ataggatagt acaggttatg cttggcagag tcaggcagtc gagggaaccg
       61 agtggaccga gaaccggccc ccggacgcca catcaggtta gaagacgtgt tgcgaccggt
      121 cgcaagcgcc ttctcgccct tacctcgtga tcctcgccgc cgtcacgggc cccgaatagc
      181 gcacagcaac ttcttacacc ttcctcccca ccggggtggt gtactcctcc gagctcacgg
      241 tatccaggtc gtgcgcccgc atatcgtctg ggacgctgtg gattcaggat cctagcacca
      301 acgtcagacg gaccacctcg gccgggtcag tcgaaggtga cgatacgcac ggggtcgagc
      361 tcgactcgta cttcggaggg ggcaacgccc cgctctttca gctgcgactc tagtgggtcc
      421 ggatcgggga ccggaagcgg cccttccatg aggacgacga cgcggccctt gcgggtggtg
      481 atatcggccg tggaccagcc tacctccttg ccgaacgccc tggccgcatc cgacaccctg
      541 gcctcgcgca ggctgtcgcg ggcaatactg accgaggtgg cggttagcgg aaccacgacg
      601 gccacgatca tggcggcaat ggcaatgacc gcgttgcggc ggttgacggt acgttgtccg
      661 gggctatcgg gggaggccgc cagcctgtgt accccgtata tcgccatcac cacggtcccg
      721 ctgccgagga tcgcggcgac gtttgtcaga aatagcagga gcgcccccag cgactggtcc
      781 ggtgcgccag actcgaaggt gaggccgacc acgctcaagg gcggcacgag cgagatggcg
      841 atggcgacac cgggcagggt gtcggagatg tcctgacgaa cgagggccac ggagcccacg
      901 gcaccggtgg ccagcgccgc gagcagatct atgaggcgcg ggtggacgcg cccggcgacc
      961 tgcgagttgg tctcggccac gacaccggtt acggaggtca gtcctagcag gtacccgatg
     1021 actacggcga ccgcggcccc gctgacgacg aggaagagcg agcgggtcag gttcgcccgg
     1081 tcggcgatca ggaccgataa catggtgccg agtatcgggg tcatcagcgg cgcgacgatc
     1141 atggccccta tcacggtcgc cgtcgagtcc gcgacgacgc cggctgccgc aatgatggag
     1201 gccagcacga tcaggatcca gaaccggctc aacctgcgtc ctcgctccgg accctcgaag
     1261 aagaccgcat cgcgcatgtg ctcgagacta ttcatcctcg atagccagtc ctgcggggca
     1321 cgctcacgct ggtgtcatcc gaggccctga aagtgtgatg cgccatttcc ggtagagccg
     1381 tccactctcg tctgatccgc tcctcacaag ggtgcctcgg ctctgtagcg gagcgttact
     1441 tgctctcgta tgccccgagc ggcgccgaga acacccccga gaagccgtac tccagcgatc
     1501 cggccgcccc aggcagcgac tccgtggtcg actacctggc ctctttcagg cacattccac
     1561 cccgcaacga gtctgctcga gggtgcaagt aaaccctgag gtgccagcat cccagcctcc
     1621 tgccaccgac tcgagctcct ggtcggagag ctctccgctt tctccgagcg gcgaggagtt
     1681 gggtagcacg aggtagatgg tctgtgccgt ctcctccagt gctaccaccc gcacatcctc
     1741 gggaagccgg gtgccaagct cctgctccac ggcggccttc gggtcggaga gcagcctctg
     1801 cctgaagacg tcgtcttcgg tgcttctctg tacgagcctg tgctccatct ccgcccaact
     1861 cgacgcttcg ctcatgggct ttccccttcc gctctaggcc gaacaccctt gcgtcctcgc
     1921 ttcgaaatac gggacacgag ggaaagtatc ccatgctagc ctacgccgag caaaaacttg
     1981 agtggtatac agacttctca gatccgaccg ggcgaacctt gcagaagaat catttcatga
     2041 cagactttct ggctcgaaag gaatagccct gtccttctgg agcattcccg ttcgtcgtac
     2101 tagcgttgag acccgataga ggaggagata tgcgctacat gctcttgatc tgcgaggacc
     2161 cgtccgccga ggcggttccc gaggacggcc ttggtgccgc gaccgagacc tgggtcgagg
     2221 agatggacgg tcggggcgtg cgcaaggtcg gtgaccgcct gcgccccgcc agcgacgcca
     2281 ccaccgtgcg ggtacgcgac gaaggggtgc tactctccga cggcccgttc gccgagacca
     2341 gggagcagat cgcaggcttc gacctggtcg agtgcgagga cctggacgag gcagtcgagg
     2401 tcgcctccag gcaccccatg gcgaggtacg ggatgatcga ggtcaggccg ttctgggagg
     2461 cgtaaggcaa aacgtcgaac gggccgtcgc ccgggcccat cgtgaggaat gggggcggat
     2521 cgtggcgacc ttgatccgta ccaccggcga ctgggacctg gcagaagaat gtgcccagga
     2581 ggccttcgca cgggcactcg agacctggcc gcgcgacggc gtcccgcgcc gacccggggc
     2641 atggctcacc acgacggccc gcaaccgggc ccgcgacttc ctgcggcgca gggcgatcgg
     2701 gtcagccagg cttggggagg tggctttgtt atccggccag gacgaacctg gcagcgacgt
     2761 gaccgagggt agcggtgtcc aagacgatcg gctgcgtctg atcttcacct gctgccaccc
     2821 cgcgctgtct ttggaggcca gggtcgccct caccctgagg accctcgcgg gcctcaccac
     2881 cccggagatc gcccgcgcgt tcctcgtctc tgaacagacg atggcgaagc ggttggtgcg
     2941 agccagggcc aaaatccgca acgcccgcat cccctaccgg gtgccgcgcg cccacctgtt
     3001 gcccgagcgc accggtgggg tactcgccgt tctctacctg ctctttaacg agggctacgc
     3061 tgccacagct ggtgccgacc tggttcgagc aggcctgagc gacgaggcta tccgactgtc
     3121 cagcaccctc gcccgcttga tgccggacga gcccgaagcg ctagggctgc tcgcgctcat
     3181 gctcctgcaa gatgcccgcc gcgccgcccg cacggacggg gctggcgacc tgataccact
     3241 ggatgagcag gaccgctcgt tgtgggaccg ggcagccatc gcggaggggg tccgcctgct
     3301 cgacgcggct cttcgcctcg ggaggcccgg tccctaccag gtgcaggcgg cgatagccgc
     3361 ctgccacgcc acagcccccg acgcctcctc caccgactgg gacgagatca ccgtcctct
//
LOCUS       DBNGVD010000599         2425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1626984, whole genome shotgun sequence.
ACCESSION   DBNGVD010000599 DBNGVD010000000
VERSION     DBNGVD010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1626984"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1210
                     /locus_tag="AC16CM_16065"
     CDS             167..1210
                     /locus_tag="AC16CM_16065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3141398.1"
                     /translation="MDQQKVLITGGAGFLGINLARHLLSRGYAVASLDVEDFDYPERD
                     RVEVIRGDIRNRAVVDRAVEGVDFVVHTAAALPLYSPEDIYTTDVEGTRNMLEASLRH
                     NVGRFVHVSSTAVYGIPDHHPLYETDKLEGVGPYGQAKIQAEMICLEYRARGLTVPII
                     RPKSFIGPERLGVFALLYDWAYTGHNFPMIGSGNNHYQLLDVEDLCDAIALTLTMREE
                     QVNDTFNIGAAEFTTMKEDYQAVLDYAGHGKKVVGFPATPAIWGLRILDRLGVSPLYK
                     WVYETASKDSFVSIEKAQRQIDYAPKFSNKEALIRNFRWYLDNLKEFENASGVSHRVP
                     WKQGAIGVLKRVF"
     gene            complement(1318..2031)
                     /gene="pgl"
                     /locus_tag="AC16CM_16070"
     CDS             complement(1318..2031)
                     /gene="pgl"
                     /locus_tag="AC16CM_16070"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3141399.1"
                     /translation="MNVDVYDNPEELARAAAREFAARAVEAIEERGRFAVVLAGGSTP
                     KATYEILASDYAEAIAWEEVHVFFGDERCVPPDSDDSNYKMARESLLDHVQIGSVHRM
                     QGELPPDEAAEAYERELHDFFGPADLPGFDLILLGTGDDGHTASLFPETSALEVHDRW
                     TVANPVLKLETTRITLTVPAINAARAVYFLVAGEGKAAPVAEILEGDPDPRQYPASLV
                     QPENGPTWMLDRAAASRLT"
     gene            complement(2028..>2425)
                     /locus_tag="AC16CM_16075"
     CDS             complement(2028..>2425)
                     /locus_tag="AC16CM_16075"
                     /inference="COORDINATES: protein motif:HMM:NF042449.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpcA/G6PD domain-containing protein"
                     /protein_id="HIR3141400.1"
                     /translation="GGENRALLLVGWLASTLGWTPESTTRDGGDQTVEFSGPSGRITV
                     ELSPDSPDARLRRIRLYSKEYSFQVSRHRANSDVRTTVMRGEDLLAERTVHIGSFDLG
                     VLVGEEMGYRGHDEAYEAALRTAVEVLDL"
BASE COUNT          415 a          745 c          810 g          455 t
ORIGIN      
        1 cgaccgaaac cccaggagct gacagctgat agctccgttc catacgcggc cggggtgttg
       61 ttggaccggg ctgcgccgtc atgcccccga cgatggatgc taaaattcgc cgtttgcgcg
      121 ggccaccgtg cgccggacat atatacgcac aaggagcatt accatcatgg accagcagaa
      181 ggttctcata acgggcgggg cggggttcct ggggataaac ctcgcgaggc acctcctgag
      241 cagggggtat gccgttgcct cgttggacgt ggaggacttc gactatcccg agcgagaccg
      301 ggtcgaggtc atcaggggtg acataaggaa cagggcggtt gtcgacaggg cggtagaggg
      361 cgtggacttc gtggttcaca cggcggcggc tttgccgctg tactcgccgg aagacatcta
      421 caccacggat gtggagggga cgcgcaacat gctcgaggcc tccctgcgcc acaacgtcgg
      481 gcgtttcgta cacgtctcat cgacggccgt gtacggcatc cccgaccacc atccgttgta
      541 cgagacggac aagctggagg gcgtcgggcc ctacgggcag gccaagatac aggccgagat
      601 gatctgcctg gaataccggg ccaggggact taccgttccg atcatccgtc ccaagtcgtt
      661 tatagggccg gagcggctcg gcgtgttcgc gttgctctac gactgggcgt acacggggca
      721 caactttccg atgatcgggt cgggaaataa ccactaccag ctactcgacg tcgaggacct
      781 atgcgacgct atcgccctga cgctcaccat gcgcgaagag caggtcaacg acaccttcaa
      841 cataggcgcc gccgagttca ccacgatgaa ggaggactac caggcagtcc tggactacgc
      901 cggccacggc aagaaggtgg ttggcttccc cgcgacgccg gccatctggg gcttacgcat
      961 cctggaccgg ctcggggtct cgcccctgta caagtgggtg tacgagacgg cctccaagga
     1021 ctccttcgtc tccatcgaga aggcccagcg gcagatcgac tacgcgccca agttctcgaa
     1081 caaggaggcc ctgatccgga acttccgctg gtacctggat aacctgaaag agttcgagaa
     1141 cgcctcgggc gtctcccacc gggtcccgtg gaaacagggt gccatcggcg ttttgaagcg
     1201 ggtgttctag gcggtacgtc gtcgggcggt ttgcgaaccg cccctacagg tttatgggga
     1261 tggtgacgat gcgtaggggc gggtccgaaa cccgccctta cgggggtttg tcgaccgtta
     1321 cgtcaacctt gatgcggcgg cccggtctag catccaggtt ggtccgttct cgggttggac
     1381 aaggctggcg gggtactggc gtgggtcggg atctccttcg aggatctcgg ccaccggtgc
     1441 tgccttgcct tcgccggcca ccaggaaata tacggccctt gcggcgttta tggcggggac
     1501 ggtaagggtg atgcgggtgg tttcgagctt gagtaccggg tttgctacgg tccagcggtc
     1561 gtgcacttcg agggcggagg tctcggggaa gaggcttgcg gtgtggccat cgtcgcctgt
     1621 gccgagcagg atcaggtcga accccggtag gtcggcgggc ccgaagaagt cgtgtagctc
     1681 ccgttcgtag gcctccgccg cctcgtccgg cgggagctcg ccttgcatcc tgtggacgct
     1741 cccgatctgg acgtggtcga gcagggactc cctggccatc ttgtagttcg agtcgtcgct
     1801 atcgggaggc acgcaccgtt cgtcgccgaa gaagacgtga acctcttccc aggctattgc
     1861 ctccgcgtag tcgctggcca ggatctcgta cgtcgctttc ggggtcgagc ccccggcgag
     1921 gaccaccgcg aaacgtcccc gctcttcgat ggcctcgacg gccctggcgg cgaactcgcg
     1981 ggcggcggcc cgtgccagct cctccgggtt gtcgtagacg tcgacgttca caggtcgagc
     2041 acctccacgg ccgtgcgcag ggcggcctcg taggcctcat cgtgtccccg gtagcccatc
     2101 tcctcaccga ccagcacccc caggtcgaag gagccgatgt ggacggtccg ttcggcgagc
     2161 aggtcctcgc cacgcatcac ggtggtcctc acgtccgagt tggcgcggtg gcgtgagacc
     2221 tggaagctgt actcttttga gtagagccgg atgcgcctca gcctcgcatc cggtgaatcg
     2281 ggggagagct ctacggtgat cctgccggag ggaccggaga actctactgt ctggtccccg
     2341 ccgtctctgg tcgtgctctc cggcgtccat cccagggtgg aggcaagcca gccgacgagc
     2401 aggagggccc tgttctcgcc gccag
//
LOCUS       DBNGVD010000600         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_714693, whole genome shotgun sequence.
ACCESSION   DBNGVD010000600 DBNGVD010000000
VERSION     DBNGVD010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_714693"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="AC16CM_16080"
     CDS             <1..166
                     /locus_tag="AC16CM_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3141401.1"
                     /translation="SPKSAFDWWMNSSGHRANILDPDFRQVGIGVRKGNFQGHPDASL
                     YTVDFGVRRR"
     gene            complement(372..>1674)
                     /gene="mutL"
                     /locus_tag="AC16CM_16085"
     CDS             complement(372..>1674)
                     /gene="mutL"
                     /locus_tag="AC16CM_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3141402.1"
                     /translation="ADDLASVVSLGFRGEALHAIGAVSHLTLTTRERGADDALATRVR
                     VAAGEMVESATVAHSLGTTVEARNLFFNLPVRRGFLGTLRSEANAVSTVVEALALSRP
                     DVGFSLRSDGRRTLALPAAADLRERVAQVHGLPLARSLVALEDPVVWGFVSPITVSFP
                     TRRYLHVAVNGRVVEADSFAPAVARAYADLLPKGRHPAAFLRLELGPGEVDVNVHPAK
                     SAVRLRGGRSAYPLVVGTIRSALAQEQAVGGAAPPEEAGDELALIGQFAGRCIVAQRG
                     EELIVLDQHGAHERILYERLSKSLRAEPVPLPSPVVARLPEDLAPEAWNFDAELEALG
                     FRFEPFGDGAVRVTAVPASVTDPETAFLAALHALAGGEDLAKALACKGSTRFGDSLSR
                     EEMENLLKEWSLTEFGEICPHGRPIVKRLALPDLLREFGRL"
BASE COUNT          281 a          571 c          576 g          246 t
ORIGIN      
        1 atcaccgaag agcgcattcg actggtggat gaatagctcc ggccacaggg ccaacatact
       61 tgacccggac ttccgacagg tcggcatagg agttcggaag ggcaactttc agggtcatcc
      121 cgatgcctcg ctgtacaccg tggacttcgg ggtccgtcgc cgctgacacg cggcgccccg
      181 tccatggtga ccatcgtgcc agcggacgcc ggctctcggt cgagcgggtg cggaacatag
      241 gccaggctcg aatgacggac tcgttcgcgg ctccaaagcg tagggcgtcg tgagcatccg
      301 atcgaccagt tccacatcga cctcccccga gcgctcggcc tgggcccaag ttcgacatgg
      361 ttgcgagctt cctagagtct tccgaactcg cgcaatagat cggggagcgc cagacgttta
      421 acgatcggcc ttccgtgggg gcagatctcg ccgaactcgg tgagagacca ttccttcagc
      481 aagttctcca tctcctccct ggagaggcta tccccgaacc tggtcgagcc cttgcaggcg
      541 agcgctttgg ccaggtcttc gccccccgcg agggcgtgca gggcggcgag gaaggccgtc
      601 tcggggtcgg taacagacgc cggtacggcc gtaacgcgca cggcgccatc accgaaaggc
      661 tcgaatcgga aaccgagggc ctcgagctcg gcgtcgaagt tccaggcctc cggggcgagg
      721 tcttcgggca gtcgcgcgac gacggggctg gggagcggca cgggctcggc tctcaggctc
      781 ttcgagaggc gctcgtagag gattcgctcg tgggcgccgt gctggtcgag gacgatcaac
      841 tcctctcccc tctgggccac gatgcacctg cccgcaaact ggccgatgag cgcgagctcg
      901 tctcccgcct cctcgggcgg tgctgcgccg ccaaccgcct gctcctgggc gagcgccgac
      961 ctgatggtgc cgaccacgag cgggtaggcc gagcggcccc cgcgcaaccg cacggcgctc
     1021 ttggccgggt gcacgttcac gtcgacctcg ccaggcccaa gctcaaggcg caggaacgcc
     1081 gcgggatgac gccccttggg caacaggtcg gcgtaggccc tggcgaccgc cggggcaaag
     1141 gagtcggcct cgaccacccg accgttcacc gccacgtgca ggtagcggcg ggtcgggaag
     1201 ctcaccgtga tcgggctgac gaagccccag acaacggggt cctccagggc aaccaggctc
     1261 ctggcgagcg ggaggccgtg cacctgggct acgcgctccc tgaggtccgc ggccgcgggg
     1321 agcgccagcg tgcgccgccc gtccgagcgg agcgagaacc ccacatcggg gcggctcagg
     1381 gcaagagcct cgaccacggt tgagacggcg tttgcctccg acctcagggt gcccagaaaa
     1441 ccccggcgca cgggcaggtt gaagaacagg ttccgcgcct caaccgtcgt gccaagagag
     1501 tgggccaccg tcgccgactc gaccatctct ccggcggcga cccgcacgcg ggttgccagg
     1561 gcatcgtccg cgccgcgttc gcgggtcgtc agcgtgaggt gggagacggc gccgatggcg
     1621 tgcagggcct caccgcgaaa gcctagagag accacgctcg cgaggtcgtc ggcg
//
LOCUS       DBNGVD010000601         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1627323, whole genome shotgun sequence.
ACCESSION   DBNGVD010000601 DBNGVD010000000
VERSION     DBNGVD010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1627323"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16CM_16090"
     CDS             complement(<1..197)
                     /locus_tag="AC16CM_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR3141403.1"
                     /translation="MRKLVVLLAVVLGVLATAVPAWAITGNYVKDFEHRYVGLVTFYD
                     DEGEYSHRCSGSLLTPRVFLTA"
     gene            complement(287..1057)
                     /gene="kduD"
                     /locus_tag="AC16CM_16095"
     CDS             complement(287..1057)
                     /gene="kduD"
                     /locus_tag="AC16CM_16095"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HIR3141404.1"
                     /translation="MGILDDFKLDGKVALVTGAARGLGQGIAVGLAEAGADVAGLDIA
                     SVDETGDLVRNEGRRFHAMNVDLLEIDAGLAADIVAECVGSLGRLDILVNNAGIIRRA
                     PAIEFGEDDWESVIKVNLSAAFYLSQAAARHFVEEDRGGKIINTASVLSYEGGIVVPS
                     YSASKAGIANLTRALANEWAASGINVNAIAPSYFATELTGALREDVERSEALLARLPA
                     GRFGEPEDLKGAVVYLASDASAYVHGTVLPVDGGWLAR"
     gene            complement(1057..1542)
                     /locus_tag="AC16CM_16100"
     CDS             complement(1057..1542)
                     /locus_tag="AC16CM_16100"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HIR3141405.1"
                     /translation="MTQEQTERLISDLGLPDQAIQSDELPWVPQGDRVWFKPLRFDLA
                     TGRWINILKIGGSGKVNRHRHTGGQVMGFCLKGSWHYLERDWVARPGTLIYEPPGDIH
                     TLVVDEGVEEMQTLFILEGTVQYIDDNDDLIYQDDVFSKMDRYLRFCEEQNIEPRDLR
                     Y"
     gene            complement(1555..>1890)
                     /locus_tag="AC16CM_16105"
     CDS             complement(1555..>1890)
                     /locus_tag="AC16CM_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3141406.1"
                     /translation="PVRWLRVGDLQLHLFLDEDPAPERHHFALDVDDFEAAFRKAGEL
                     GVRDGGRHSSVRELPGGAVQMYLRDPAGNLVEVNWPDVTTLDRGLIPEIQKIGGPEDA
                     VLYLEPKVG"
BASE COUNT          319 a          616 c          608 g          347 t
ORIGIN      
        1 gcggtcagga acaccctggg cgtgagcaac gagccagagc atcggtggct gtactcacct
       61 tcatcgtcgt agaacgtcac cagcccgacg taccggtgct cgaaatcctt gacgtagttg
      121 cccgttatcg cccaggcagg caccgcggtt gccaacacgc ccagcacgac ggccagaagc
      181 acgacgagtt tccgcatgca tttcctcctc tggcattatt tcggcccgca gcggatgcgg
      241 cgggcgcaca atcctagcat gcgaactgct gggtgggcaa gacttcctac cgcgccagcc
      301 atcctccgtc cacgggaaga accgtgccgt gtacgtaggc ggaggcgtcc gaggcgaggt
      361 agaccacggc gcccttgagg tcctcgggct cgccgaaccg gcccgcgggc aggcgtgcca
      421 gcagggcctc tgacctctcc acgtcctcgc gcagcgcgcc ggtcagctcg gtggcgaagt
      481 agctgggggc gatggcgttc acgttgatgc cggaagccgc ccactcgttg gccagggcgc
      541 gggtcaggtt ggcgatgccc gctttggagg ccgagtagga gggaacgacg atcccgcctt
      601 cgtaggacag gaccgaggcg gtgttgataa tcttgccccc acggtcttcc tcgacaaagt
      661 gcctggcggc ggcctgagac agatagaagg cggcgctgag gttgaccttt atgacgctct
      721 cccagtcgtc ctcgccgaac tcgatggcag gggcccgccg gatgatgccg gcgttgttaa
      781 cgagtatgtc gagacgcccg agcgagccca cgcactccgc tacgatgtcg gcggcgagtc
      841 ctgcgtctat ctccagaagg tccacgttca tggcgtgaaa acggcggcct tcgttccgca
      901 caaggtcccc ggtctcgtct accgacgcga tatccaggcc ggccacgtcg gcgccggctt
      961 cggcgaggcc cacagcgatt ccctgaccca ggccgcgtgc tgccccggtt acgagggcga
     1021 ccttgccgtc gagcttgaag tcgtcgagga tacccattag tagcggagat cccggggctc
     1081 gatgttctgc tcctcgcaga agcggaggta gcggtccatc ttggagaaca cgtcgtcctg
     1141 gtagatcagg tcgtcgttat cgtcgatgta ctggacggtg ccttcgagga tgaagagcgt
     1201 ctgcatctcc tcgacgccct cgtccacgac gagcgtgtgg atgtcgcccg gcggttcgta
     1261 gatgagcgtg cctggcctgg cgacccagtc tcgctccagg tagtgccagg agcccttcag
     1321 gcagaatccc atgacctggc cgccggtgtg gcggtgacgg ttgaccttgc ccgagccccc
     1381 gatcttgagg atgttgatcc aacgccccgt cgcgaggtcg aacctcaagg gtttgaacca
     1441 gaccctgtcc ccctgcggca cccagggcaa ctcgtcgctc tgtatggcct gatccggcag
     1501 cccgaggtcg gagatcaaac gctccgtctg ctcctgcgtc acggtcttct cctttcagcc
     1561 gactttcggt tcgaggtaca ggacggcgtc ttcggggccg cctatcttct gaatctccgg
     1621 tatgaggccg cggtccaaag tggtaacgtc gggccagttg acctcgacga ggttgcctgc
     1681 gggatcccgc aggtacatct gcacggcacc accgggcaac tctcttacgg aggagtggcg
     1741 ccccccgtcc ctgacgccga gctccccggc cttcctgaac gcggcctcga agtcgtcgac
     1801 gtcgagggcg aaatggtgcc gctccggcgc ggggtcctca tccagaaaga ggtggagttg
     1861 cagatccccg acgcgcagcc agcggaccgg
//
LOCUS       DBNGVD010000602         4641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2538525, whole genome shotgun sequence.
ACCESSION   DBNGVD010000602 DBNGVD010000000
VERSION     DBNGVD010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2538525"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC16CM_16110"
     CDS             <1..332
                     /locus_tag="AC16CM_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141407.1"
                     /translation="LARGVANAVRRGDAIAPQLPEAVLDLSRSVRALATFLEDGEGPE
                     EARNWALEAAMRATDILDEPHGLGISVLVGQVRSAAVDLLRSTGMDQASALRALEDAA
                     GRASEIG"
     gene            complement(368..766)
                     /locus_tag="AC16CM_16115"
     CDS             complement(368..766)
                     /locus_tag="AC16CM_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005045885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3141408.1"
                     /translation="MRRDNVTSGTVWESSVGYSRAVRRGRFVFVSGTTATDGSGGYVG
                     NGDPYAQTVQALNNIRVALQALGADLPDVVRTRIYVVDIEDWEKVGAAHGEFFDKIKP
                     ATSMVEVSRLIAPEILVEIEADAIVDDTVP"
     gene            complement(800..3118)
                     /locus_tag="AC16CM_16120"
     CDS             complement(800..3118)
                     /locus_tag="AC16CM_16120"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HIR3141409.1"
                     /translation="MMREKVGPEETDLRRTLTRRGFLRLAGAGVAGTALLGGACALWM
                     RPGQLSGAITGVARDVSSASYRVGAFEVLLKTGRDPSEVSLNVANSSKTGRVLWRSIP
                     GASFVSAAEGRETVRQERAHISLEDEISNEHPDQTIDRVEQRGDVLLISGRLTGADDP
                     GSVDYTFTLSPAGDGRLSFEAEVGEPCNRVYLTCASSPEERFFGFGTQFTYFDMKGHV
                     VPILIREQGIGRGEQPVTWAADWKAGAGGDPYTSYASVPHYITSEARSLFLENYEYST
                     FDLTERDRVQAGVFAPRMRGQIPSAQTPEGLIEQYTAYAGRMRPLPEWIMGGAVVGLQ
                     GGTDRVREILERLEALDAPVAAVWLQDWVGQRETGFGTQLWWNWELDRDHYPDWGGLV
                     ESLKRKNIRLMTYINPFLCDDVGLKKDYRRDLFREAMEGGYLVENRDGGPYRIRISDF
                     SAGLVDLTNPGARTWIKEIIREELIGNGASGWMADFGEGLPYDAVLFSGADPRTYHDR
                     YAEEWARVNREVIREAGREDDIVFFNRSGYIQSPRYSTLFWVGDQLVDWGEQDGIKSA
                     VTGLLSSGLSGYSLQHSDIGGYTAIDNPLLRYHRSRELLLRWVELAAFTTVFRTHEGN
                     IPEVSHQFYSDEETLEHFVRFCRVYATWKPYRMKLVKEASERGLPLVRHPFIHYPEDP
                     EVLALEYQFMVGAELMVAPVLDPGTDAVDVYLPAGGWVHLWTGNKYGSPDRGTYKTFP
                     APMGEPAVFFRAGSAEGLGFREELRRKGLLRG"
     gene            complement(3240..3905)
                     /locus_tag="AC16CM_16125"
     CDS             complement(3240..3905)
                     /locus_tag="AC16CM_16125"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3141410.1"
                     /translation="MSEDRFEMLLGPYVLGELTEEEERELEHHLEGCRTCQGDLDVLR
                     SADGLLRAASAGPPPELKDWVLARVRNEERSATGSKWRVWGVVAASLLVVALLSFGAL
                     RTLTGPSEALALTATDAAPQAGGELRGERIGDNLKVELDAWGLPRPEDGGYYEMWYAR
                     SDGGRVSCGTFTVQQDGSATVSMSAPLSSVAYPQVEVTREPDDGDPGSSGKVVLKGSL
                     RDL"
     gene            complement(3898..4446)
                     /locus_tag="AC16CM_16130"
     CDS             complement(3898..4446)
                     /locus_tag="AC16CM_16130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3141411.1"
                     /translation="MGDEKLVVLMAGGEERALSELYDRYSRPVYATGLRLLGDKQLAE
                     ELVQDAFTSVWRNAASFDQARASFATWLYRIARNRATDMDRRRRVRPRPAGEHGLYAI
                     TGGPRPEEAVDAWDVAAALSTIPTQYRQVLEMAYFEDLSQREISRRTGIPLGTIKSRT
                     TAALKRFRQAIANPAPKEARGE"
BASE COUNT          751 a         1585 c         1446 g          859 t
ORIGIN      
        1 tcctcgcccg cggcgtcgcc aacgccgtca ggagggggga cgccatcgcc ccgcaactgc
       61 cagaggccgt gctagacctc tcgcgatcgg tgagggctct ggccaccttc ctggaggacg
      121 gcgagggacc cgaggaggcc cgcaactggg cgctcgaagc ggccatgcgg gcgaccgata
      181 tcctggacga gccccacggg cttgggatca gcgtcctcgt tgggcaggtc agatccgcgg
      241 ccgtagacct cctcagaagc accgggatgg accaggcctc cgccctcagg gccctggagg
      301 acgccgctgg ccgcgcctcg gagatcgggt aggggcgagt tcgaaacccg cccacgacgg
      361 gcggcaccta cggtaccgtg tcgtctacta tggcgtcggc ttcgatttcg accaggatct
      421 cgggggctat caggcggctg acctctacca tgctggtggc cggctttatc ttgtcgaaga
      481 attcgccgtg ggcggcgcct accttttccc agtcctcgat gtcgacgacg tagatacggg
      541 tccgtaccac gtcggggagg tcggcgccca gagcctgtag ggctactctt atgttgttca
      601 gggcctgcac ggtttgcgcg taagggtcac cgtttcccac gtatccccca cttccatccg
      661 tcgccgtggt gcccgagacg aacacgaacc gcccgcgcct gacggcccgc gaatagccga
      721 cgctgctctc ccacaccgta ccgctggtta cgttgtcccg tctcatgccg aatccctcat
      781 gaggagcatc tcgtatattt taccctcgta gcaggccctt acgccggagc tcctccctga
      841 aaccgagtcc ctccgcggag cctgctcgaa agaagactgc tggctctccc atgggcgccg
      901 ggaaggtctt gtatgtgcca cggtcgggcg atccgtattt gtttcccgtc cagaggtgga
      961 cccagcctcc ggcgggaagg tagacgtcta cagcatcggt gccggggtcc agcacaggcg
     1021 ccaccatgag ctcggcgccg accataaact ggtactcgag ggccagtact tcgggatctt
     1081 ccgggtagtg gatgaaggga tgccttacga gggggagccc tctttccgac gcctccttga
     1141 cgagcttcat cctgtagggc ttccaggtcg cgtagaccct gcagaagcgt acgaagtgct
     1201 cgagggtttc ctcgtccgag tagaactggt ggctgacctc gggtatgttg ccctcgtggg
     1261 tacggaacac ggtcgtaaag gcggctagtt cgacccaacg caacagcaac tccctggagc
     1321 ggtggtaccg gagcagggga ttgtctatgg cggtgtatcc accgatgtcc gagtgctgga
     1381 gactgtaacc cgagaggccg ctgctcagca ggcccgtgac ggcgctcttt atgccgtcct
     1441 gctcgcccca gtccacgagc tgatctccta cccagaacag ggtgctgtag cgagggctct
     1501 gtatgtaacc ggagcgattg aagaacacga tatcgtcttc ccggcccgcc tcgcggatca
     1561 cctcgcggtt caccctggcc cattcctcgg cgtagcggtc gtggtaggtc ctggggtcgg
     1621 cgccggagaa cagcaccgcg tcgtaaggaa gtccctcccc gaagtcggcc atccaccccg
     1681 aggccccgtt gccgatcaac tcctccctga ttatttcctt gatccaggtt ctcgcccccg
     1741 ggttggtgag gtccacgagg ccagcggaga agtcggagat cctgatcctg tatggtccgc
     1801 catcgcggtt ctcgaccaag tagccacctt ccatggcctc ccggaagaga tcgcggcgat
     1861 agtctttctt gagccccacg tcgtcgcaga gaaaggggtt gatgtaggtc atgagcctga
     1921 tgttcttccg tttcaggctc tcgacgagcc caccccagtc ggggtagtgg tccctgtcga
     1981 gctcccagtt ccaccacagc tgggtgccga agcctgtctc cctctggccc acccaatcct
     2041 gcagccagac ggcggcgacg ggggcatcaa gggcttcgag tctttcgagt atctcgcgga
     2101 ccctgtccgt gccaccctgc aaccccacca cggccccgcc catgatccat tcggggagcg
     2161 ggcgcatcct tcccgcatac gcggtgtact gctcgatcaa gccctcgggt gtctgtgccg
     2221 aagggatctg ccccctcatc cggggggcga agacgcctgc ctgaactcta tccctctccg
     2281 tgaggtcgaa ggtcgagtat tcgtagttct ccaggaacag ggagcgcgcc tcgctcgtga
     2341 tgtagtgcgg cacgctcgcg tagctggtgt aggggtcgcc gccggcgccg gccttccaat
     2401 cggcggccca ggtcacgggc tgttcgcccc tgcctatgcc ctgctccctg atgaggatgg
     2461 gcacgacgtg ccccttcatg tcgaagtacg taaactgggt cccgaagccg aagaagcgct
     2521 cttccgggga ggaagcgcag gtgaggtaca cccggttgca aggctccccc acctcggcct
     2581 cgaaagagag cctcccgtcg ccagctggtg aaagcgtgaa cgtgtaatcg acgctcccgg
     2641 gatcgtccgc gccggtaagc cggccggaga tcagcaacac atcgccgcgc tgctcgaccc
     2701 tatctatggt ctggtcggga tgctcgttag agatttcgtc ttccagggat atgtgcgccc
     2761 tctcctgccg aacggtctct ctaccctcgg cggcggagac gaaactggcc ccgggaatgc
     2821 tgcgccagag caccctccct gtcttggagc tgttggctac gttgagggag acctcagagg
     2881 gatcacggcc ggtcttcaac agaacctcaa acgcgccgac gcggtaggag gcagaggaga
     2941 catctctagc caccccggta atggcgccgg agagctggcc cggtcgcatc cagagagcac
     3001 aggccccacc gagcagcgcc gttccggcca cgccggcgcc ggcgagcctc aagaatcccc
     3061 tgcgcgtcag cgttcttctc agatccgtct cttccggtcc aaccttctcc cgcatcatac
     3121 aggagcacca gaaccggccg tccagggtta gaggttcgac tccaaccggc gaacgcacgc
     3181 gaaagacccc ttaggggcgg ttcgcgaacc gtccgtgcaa cccgcgatgt gtgacaccgt
     3241 cagaggtcgc gcagcgaccc tttcagcacc acctttccgc tggagccggg atctccgtcg
     3301 tcgggctccc gggttacctc gacctgggga taggccaccg agctgagcgg cgcggacatg
     3361 ctgacggtcg cgctaccgtc ctgctgcacg gtgaaggtac cgcagctgac gcgtcctccg
     3421 tctgatctgg cgtaccacat ctcgtagtat cccccgtcct ccggcctggg caggccccag
     3481 gcgtcgagct cgaccttgag gttgtcaccg atccgctctc cccgcagctc accgccggct
     3541 tgcggggccg cgtcggtagc ggtcagggct agtgcctcgg aggggcctgt tagtgttcgg
     3601 agcgcaccga agctcaggag agcgaccacc agcagcgagg cggccaccac gccccagacc
     3661 ctccatttgc tccctgtagc actgcgctcc tcgttccgca cgcgtgccag cacccagtcc
     3721 ttcagttccg gtggcgggcc cgccgaggcg gcgcgcagca ggccgtcggc gctgcgaagt
     3781 acgtcgaggt cgccctggca ggtgcggcat ccttcgaggt ggtgctccag ctcccgctcc
     3841 tcctcttccg tcagctcccc tagcacgtac gggccgagca gcatctcgaa gcggtcttca
     3901 ctcaccgcgg gcctccttcg gcgcggggtt ggcgatggcc tggcggaacc gcttcagggc
     3961 ggcggttgtt cggctcttga tcgtcccgag gggaatgccg gtgcggcggg agatctcccg
     4021 ctgcgagaga tcttcgaagt aggccatctc cagcacctgc ctgtactggg tgggaatggt
     4081 cgagagcgcg gcggccacgt cccaggcgtc gacggcctcc tcgggacgag gtccgccggt
     4141 gatagcgtag agcccgtgct cgcccgccgg ccttggcctg acccgccttc ttcggtccat
     4201 atccgtggcc ctgttgcggg cgatgcggta gagccacgtg gcgaagctcg ccctggcctg
     4261 gtcgaaggac gccgcgttgc gccagacgct cgtaaaggcg tcctggacca gttcctcggc
     4321 caactgcttg tcgccgagca gccgcaagcc cgtggcgtac accggccgcg agtagcgatc
     4381 gtagagttcg gagagcgccc gctcttcccc gccggccatc aggaccacaa gtttctcatc
     4441 ccccacttcg tgcaggttct gtccgaggcc ccaccccgcc ccgtaatgtc gtttcgtccg
     4501 cccgatagaa tgcccccgaa ccttgccctg gaggtggcca cctacagcct accaccggga
     4561 ccccggtcgg cacgtgccgg ccggggtccc aaccgtcggg gcgctaggga ctgcatcccc
     4621 cctcgaccgc gtcttccccg a
//
LOCUS       DBNGVD010000603         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4179003, whole genome shotgun sequence.
ACCESSION   DBNGVD010000603 DBNGVD010000000
VERSION     DBNGVD010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4179003"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..587
                     /locus_tag="AC16CM_16135"
     CDS             102..587
                     /locus_tag="AC16CM_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR3141412.1"
                     /translation="MKSPEGVHQVRAWSYENIVLESYRYAPGPAATLPRHSREDYQFC
                     LSLDFPGEYRYRGSSFGVPVGSLSIIHPGEMRSARDPEDRRKAANYRLVYVGRDLLRR
                     AAPDVAGRTTGEPFFPPIVLDGGLIGRFQKLHASFATRSLPTSPRSQAGDRAGCPPTT
                     W"
     gene            complement(848..2137)
                     /locus_tag="AC16CM_16140"
     CDS             complement(848..2137)
                     /locus_tag="AC16CM_16140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3141413.1"
                     /translation="MFDHIRKRLTMGYVGILALILLVFGAVVMFSFQRQVTIQQDKLL
                     DQKVKSLVNGSLSDTDKIAILEDPRSYGWVILTPRGRTLGQNPTAASLGLPAARLARE
                     SLHEQQTVSATVRGPQGEVRVASSPAVQSGSLVVLQVARSPAVVQGIVARLAAVLVFT
                     GLGALVLVAAGGLFMSRRAMRPVQEAFGRQRTFIADASHELKTPLTLIRADAEVLSRG
                     LDDPEHAADNRELVDDLLGETDRMSAILSDLLLLARLDAEKISVSREPFDLALVLSET
                     SERFAARAVAEGKNLEVKHSGKFPARGDAERTGQILAALLDNALRFTPPDGTVTVEGG
                     ATDKRISATVTDTGPGIPQGSLDRIFERFYRADTHSEARTRGPSGGGTGLGLAIARDL
                     ARAQGGELAAENVPGGGARFTLTLPSSSKRPEAIRQG"
     gene            complement(2137..2820)
                     /locus_tag="AC16CM_16145"
     CDS             complement(2137..2820)
                     /locus_tag="AC16CM_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3141414.1"
                     /translation="MRILVIEDEARLARLITRVLGEEGYAVETQDNGRQALLLALAEE
                     FDLLIVDWMLPEMSGVQLVKRLRAAEVDTPVLMLTARDQIEDRVEGLDAGADDYLPKP
                     FAFPELLARVRALTRRPRGEEKEGAVLQAGDVTLDPVRHVVRNGRANVDLTAKEFALL
                     ATLMQRPGQVFTRSVLLDTVWGGSPEVYANVVDLYVSYLRKKLDRDGGSSHIRTVRGV
                     GYTFEPQPH"
     gene            2956..3807
                     /locus_tag="AC16CM_16150"
     CDS             2956..3807
                     /locus_tag="AC16CM_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3141415.1"
                     /translation="MATRHITQQEAEAPRLLAAYMARIARGDLLSHEEEIELGRRARA
                     GDPAARRRLVEKNLRLAVAVAKKFRGQGLPFEDLIQEGNIGLMKAVDKFDPDMGNRFS
                     TYATWWIRQAIQRGIGDKGREIRLPAHMNEKLRKVRKVQGELVAGLHREPTYEETAAR
                     LGWEAGKVRSILEAVPDISSLDRPLGPESGAHRLGDFVEDHESEGIEETQQLREAMRQ
                     LPERERHVLVNRYGLDRGKKVTVRELSAKLGISEKAVRRVQQRAEKLLRALLDPGTRG
                     GLGGVTA"
     gene            3804..>4191
                     /locus_tag="AC16CM_16155"
     CDS             3804..>4191
                     /locus_tag="AC16CM_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3141416.1"
                     /translation="MRGLKIRPAYMVSLTILAVLAFVLGVVYLSPGTGNTQTAEPIKS
                     APARIPADEPVARVASVLSPSVVQINVMGVQQTPYGSQKEEGIGSGVIYRSDGYIITN
                     NHVVEGSRQVEVAFADGSTEQGQVVGT"
BASE COUNT          765 a         1361 c         1402 g          663 t
ORIGIN      
        1 cgcgctacgc gaagccggct acgacgacgc ctccttgagg aagctcgcgc acgagaactg
       61 ggtcagggtg ctaaggtcga cctggcgcgg gtagatcctt cgtgaaatcc cccgaggggg
      121 tacaccaggt cagggcatgg tcttacgaaa acatcgtgct cgagtcgtac cgctatgccc
      181 ccggccctgc ggcaaccttg cccaggcatt cacgcgagga ctaccagttc tgcctcagcc
      241 tagattttcc gggcgagtac cgctacaggg gaagtagttt cggagtaccc gtaggcagcc
      301 tcagcatcat ccacccgggc gagatgcgct ccgccaggga tcctgaagac aggcgcaagg
      361 ccgcgaacta caggctggtg tacgtcggga gggatctgct ccggagggcg gccccggacg
      421 tggcagggcg cactactggc gagcccttct ttccccctat cgtcctcgac ggtggtctca
      481 tcgggcgctt tcagaagttg cacgcctcgt tcgcaacgag atctctgccg acttcacctc
      541 gctcgcaggc gggggaccgg gcaggatgcc cgccgacgac ctggtaaacg cctggaagac
      601 caacctctac gccgagaaac gcagccatca caggcgcacg aaccgcaggt tctccatacg
      661 agaagaccgg gccgaggtct tcttccaaag gctacgtcct gaacatcctc gggcgggaca
      721 cgggctctga cctgtgggaa gtatgggggt tacatacata cgctggagcg taccccagaa
      781 ggctcgagat gctcgggcgt gaccttcgtg gtcacccacg cccgcggcaa cgagatggcc
      841 cgcgagctca tccctgacgg atagcctcgg ggcgcttcga gctcgatggc aacgtgagcg
      901 tgaacctggc gccgcctccg ggaacgttct cggccgcgag ctcgccgccc tgggcgcggg
      961 ccaggtccct ggcgatggcc aggccaaggc cggtaccgcc gcccgacggt cccctggtcc
     1021 tggcctcgct gtgcgtgtcg gccctgtaga agcgctcgaa gatgcggtcc aggctcccct
     1081 gtgggatacc cggccccgtg tctgtcacgg tcgccgagat gcgtttatcc gttgccccac
     1141 cctcgacggt tacggttccg tccggcggtg tgaagcggag ggcattgtcc aggagggcgg
     1201 ccagaatctg gcccgtgcgc tccgcatcgc cgcgggctgg aaacttgccc gagtgcttca
     1261 cctccaggtt ctttccctcg gcgacggcgc gggcggcgaa gcgctccgag gtctccgaga
     1321 ggacgagggc caggtcgaaa ggttcgcggg agacggagat cttctccgcg tccagccgcg
     1381 cgaggagcaa caggtcggac aggatggcgc tcatcctgtc ggtctcgccg agcaggtcat
     1441 ccacgagctc gcggttgtcc gcagcatgct ccgggtcgtc gaggccccgg gagagcacct
     1501 cggcatcggc ccggatcagg gtcaggggcg tcttgagctc gtgggaggcg tcggcgatga
     1561 acgtccgctg cctgccgaaa gcctcctgta caggacgcat cgcccgccgc gacatgaaga
     1621 ggccaccggc cgccaccagg acgagcgcgc caaggcccgt gaaaaccagc accgcggcga
     1681 ggcgggccac gataccctgg actaccgcag gagagcgcgc gacctgcaac acgaccagcg
     1741 accccgactg aacggcgggc gagctcgcca ccctgacctc gccctggggg cccctgaccg
     1801 tcgcggagac cgtctgctgt tcgtgcaggg actcccttgc cagcctcgcc gcgggaaggc
     1861 ccagggaggc ggctgtgggg ttctgtccga gcgtgcgtcc gcggggggtg aggatcaccc
     1921 agccgtagct cctggggtct tcgagtatgg ctatcttgtc cgtatccgac agggacccgt
     1981 tcacgaggct ctttaccttc tggtcgagca gcttgtcctg ctggatcgtt acctgcctct
     2041 ggaagctgaa catcacgacg gcgccgaaga cgagcaggat gagggccagt atgcccacgt
     2101 atcccatcgt caggcgcttc ctgatgtggt cgaacatcag tgcggttgtg gctcgaaggt
     2161 ataaccgacg ccccgcacgg tcctgatgtg ggatgaaccg ccgtcgcggt cgagcttctt
     2221 ccgcaggtaa gagacgtaaa ggtccaccac gttggcgtag acctcgggcg aaccgcccca
     2281 cacggtatct agcagcaccg agcgggtgaa gacctgcccg ggacgctgca tcagggtggc
     2341 gagcagcgcg aactccttgg ctgtcagatc cacgttcgca cgtccgttgc gcaccacgtg
     2401 gcgcacgggg tcgagggtga cgtcgccagc ctggagaacg gccccctcct tctcctcgcc
     2461 ccgcggacgg cgtgtaaggg cacgcaccct cgcgagaagc tcggggaagg cgaagggttt
     2521 cgggagatag tcgtcggccc cggcgtcgag accctccacg cggtcttcga tctggtcgcg
     2581 agccgtcagc atcaggacgg gggtgtcgac ctcggccgcc ctcaggcgct tcactagctg
     2641 tacgccgctc atctcgggta gcatccagtc cacgatcagg aggtcgaact cctcggccag
     2701 ggccaggagg agcgcctggc ggccgttgtc ctgggtctcg accgcgtaac cctcctcacc
     2761 caggacgcgc gtgatcaggc gcgccagcct cgcttcgtcc tctataacca gtatgcgcat
     2821 cgtattccca ttatcgcccc tgtgcggcaa tcctgccccc gggtcacgct ttcctgtgaa
     2881 aaacctcatg aaccgcaaag gctgctcaca gactgtagcg ccagactgat ggggacagaa
     2941 ccagaaggag gaaagatggc gactcgtcat ataacacagc aggaggcgga ggccccgagg
     3001 ttactcgcgg cgtacatggc gcgcatagcg cggggcgacc tgctgagcca cgaagaggag
     3061 atagagctgg gccgcagggc ccgcgccggt gacccggcgg cccgacggcg gctcgtcgag
     3121 aagaacctca ggctcgcggt ggcggtcgcc aagaagtttc gagggcaggg cctgccgttc
     3181 gaggacctga tccaggaggg caacatcggc ctgatgaagg cggtcgacaa gttcgatccc
     3241 gatatgggca accgcttctc gacgtacgca acgtggtgga tcaggcaggc catccaacgg
     3301 ggcatcggcg acaaagggcg cgagatccgg ctacccgccc acatgaacga gaagctgcgc
     3361 aaggtgcgca aggtgcaggg agagctcgtc gccgggctcc atcgcgagcc gacctacgag
     3421 gagacggcgg cccggcttgg gtgggaggcc ggcaaggtcc gctcgatcct cgaggccgtg
     3481 ccagacatct cgagcctgga ccgacccctc ggccccgaat ccggggccca caggctcggg
     3541 gatttcgtgg aggaccacga atcagagggc atcgaggaga cccagcagct tcgggaggcc
     3601 atgaggcaac tgcccgagcg ggagcgccac gtgctggtca accgctacgg cctcgacagg
     3661 ggcaagaagg ttaccgtacg cgagctttca gcgaagctcg gcatctcgga gaaagccgtc
     3721 aggcgggtgc aacagagggc cgagaagttg ctcagagcgc ttctggaccc ggggacacgc
     3781 ggcggcctcg ggggggtaac ggcatgagag ggctcaagat acggccagcc tatatggtgt
     3841 cgctcacaat cctggccgtg ctggccttcg tgctcggtgt agtctacctg agccccggca
     3901 cgggcaacac ccagaccgcc gagccgatca aatccgcccc cgcgcgcatc ccggccgacg
     3961 agcccgtggc cagggtggcc tccgtgctca gcccatccgt ggtgcagatc aacgtgatgg
     4021 gggtccagca gacgccatac ggatcccaga aagaggaggg tatagggagc ggtgtgatct
     4081 accgctctga tggctacatc atcaccaaca accacgttgt cgagggctcg cgccaggtcg
     4141 aggtcgcctt cgccgacggc tccaccgaac agggacaggt cgtcggcacg g
//
LOCUS       DBNGVD010000604         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3449507, whole genome shotgun sequence.
ACCESSION   DBNGVD010000604 DBNGVD010000000
VERSION     DBNGVD010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3449507"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16CM_16160"
     CDS             complement(<1..296)
                     /locus_tag="AC16CM_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141417.1"
                     /translation="MLRRLLSFVVRWSGLLCVPGGALWALTPTGVYISNFAFHTPNVF
                     WKLFWPAPVLLLLGLIGLQLRQSDRSGLLQKIGFFVTALGLLMVTAGDIGLYWLG"
     gene            complement(296..1786)
                     /locus_tag="AC16CM_16165"
     CDS             complement(296..1786)
                     /locus_tag="AC16CM_16165"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3141418.1"
                     /translation="MKDRTLTRRDFLKAAGAGAAGAAMLGTAGCSPMLTNLTHVSDEY
                     LPSGGSRMNVILVIIDSLRRDHLGVYGNDWIETPNLDALAKESLIFDRSHPESLPTIL
                     ARRAIHTGIRTFPFRDWQKWYSEDVNLWGWQPIPRDQVTLAEILVKEGFFNLMVTDTL
                     HQFRPYYDMHRGFHAFDFIRGQERDFFRPQSPASEKKMENTLVGGPSAEHSAEIMRQY
                     WANTMGRKSEEDWFAPQVFTKAADYLEMAKEAQPFFMFVDNYDPHEPWDPPQKYIDLY
                     SDGFDGPEPVTSSSGPSDWLTEAQLKRMHARYSAEVTMTDHWLGHFLDKAHDLGVLDN
                     TMLIVMSDHGHAFGEHGYAGKVVSALYPELIDTIFFVRHPGGKRAGERSDHYASTHDV
                     APTILGSMGVGRHPQMQGQDLSVFFDGKEPEARPYFTAGYHDHVWTRDDKHAMFCLYD
                     GSNPYLFDLQKDPKMDKNIASANPAVVKKMFNDYIIKDAGGPLPHY"
     gene            2053..3036
                     /locus_tag="AC16CM_16170"
     CDS             2053..3036
                     /locus_tag="AC16CM_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3141419.1"
                     /translation="MNWLVTGGCGFIGTALIRTLMQEGDHGIRVVDNLAVGTREDLAA
                     AGDFVEVSPDGPGPISAGGRVELLVGDIQDAGLALGAAEGADVIVHLAANTGVMPSVE
                     DPRADCMSNVIGTLNFLEAARHNGVDRFVFASSGGTVIGDAEPPIHEEMVAHPVAPYG
                     ASKLAGEGYCSAYFQTFGIGTVALRFGNVYGPLSGHKNSVVARFLKRAMDGEVLEIYG
                     DGTQTRDFIFVDDLIRAIRLSATKDGVGGEVFQIATSAETTVQELTDRLLPAMAAAGL
                     EGVEVRNTDARRGEVRRNYADTSKAHRMLGWKAEVGLDEGLKRTVDWFVHE"
     gene            complement(3067..>3388)
                     /locus_tag="AC16CM_16175"
     CDS             complement(3067..>3388)
                     /locus_tag="AC16CM_16175"
                     /inference="COORDINATES: protein motif:HMM:NF018745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HIR3141420.1"
                     /translation="SITAMRRLPAGFDSPERGTDALDFDYYAFGVGTTFTSPEVEVTS
                     RVVRRTTLAHGAFSFFYNTAILALVVNLLIAGAAKTRRKPSRRAFLFSPSIRRPPGSF
                     QLPG"
BASE COUNT          604 a         1039 c         1122 g          623 t
ORIGIN      
        1 cccagccagt acaggcctat atctccggcc gtgaccatga gcagccccag ggcggtcacg
       61 aagaacccga tcttctgcag taggccagaa cggtctgact ggcgcagctg gaggccgatc
      121 aggccgagga gcaggagcac cggggccggc cagaacagct tccagaacac gttcggggtg
      181 tgaaaggcga agttggagat gtagacgccc gtgggggtaa gcgcccacag cgcccctcct
      241 ggcacgcaca gcaagccact ccacctgacg acgaacgaca acagcctacg cagcattagt
      301 agtggggaag ggggccgccg gcgtccttga tgatgtagtc gttgaacatc ttctttacca
      361 cggcggggtt cgccgaggcg atgttcttat ccattttcgg gtccttctgg aggtcgaaga
      421 ggtaggggtt ggagccgtcg tagaggcaga acatggcgtg cttatcgtcg cgggtccaga
      481 cgtggtcgtg gtaaccggcg gtgaagtaag gtctcgcctc gggctcctta ccatcgaaaa
      541 ataccgagag gtcctggccc tgcatctgcg ggtgccgccc cacgcccatc gagcccagga
      601 tggtgggggc cacgtcgtgg gtggaggcgt aatggtcgct cctctcgccc gcacgcttcc
      661 cgccgggatg acgaacgaag aagatggtgt ctatgagctc gggatagagc gcgcttacga
      721 ccttgccagc gtagccgtgc tccccgaagg cgtggccgtg gtcggacatc acgatcagca
      781 tggtgttgtc gagcacgcca aggtcgtgcg ccttgtccag aaagtgtcct agccagtggt
      841 ccgtcatggt tacctcggcc gagtagcgcg cgtgcatccg cttcagctgg gcctcggtca
      901 gccagtctga ggggccgctg ctggaggtga ccggctcggg tccgtcgaag ccgtcgctgt
      961 agaggtcgat gtacttttgc ggcgggtccc acggctcgtg cgggtcgtag ttgtccacga
     1021 acatgaagaa gggctgggcc tccttcgcca tctccaggta atcggccgct ttggtaaaca
     1081 cctgcggggc gaaccagtcc tcctcgctct tgcggcccat ggtgttggcc cagtactggc
     1141 gcatgatctc cgcagagtgc tcggcgctcg ggccgccgac cagggtattc tccatcttct
     1201 tctcggaggc cggtgactgc ggcctgaaaa agtccctctc ctggcccctg atgaaatcga
     1261 aggcgtgaaa gccacggtgc atgtcgtagt acggcctgaa ctggtgaagc gtgtccgtga
     1321 ccatcaggtt gaagaaccct tccttcacca ggatctcggc gagcgtcacc tgatccctgg
     1381 ggatgggctg ccagccccag aggttgacgt cctcgctgta ccacttctgc cagtccctga
     1441 acgggaaggt cctgatgccg gtgtgtatgg cgcgccgcgc caggatggtc ggcagcgact
     1501 cggggtgcga gcggtcgaag atgaggctct ccttggcgag agcatcgagg ttgggggtct
     1561 ctatccaatc gttcccgtag acgccgaggt ggtccctcct gaggctatct atgatgacca
     1621 gtattacgtt cattctcgat cccccgctcg gaaggtactc gtcggatacg tgcgtgaggt
     1681 tggtcagcat ggggctgcag ccggccgtgc cgagcatggc ggccccggcc gcgccggcac
     1741 cggccgcctt cagaaagtcc ctgcgcgtga gggtgcggtc cttcatcccg tatcctcggg
     1801 cgcggcgtgt ttctctcggg catgccgtgt tctggtatct ctttgcaagg tcttcctcgt
     1861 catgattcga atcaaccgcc ggagattgta gcgaagagcg cctccgtccg caggattcta
     1921 ctacgtcagc ccctaaactc gcaccaggcg ggaggttggt ctaatgtatg cgcggcaaac
     1981 cacgtctaga aggatgggca tgcacacgat caggaatacc ccagaacgtc ccctctcccc
     2041 ggcgcgttgc tcgtgaactg gctggtcacg ggcggctgcg gctttatcgg aacggccctg
     2101 atccggaccc tcatgcaaga gggtgaccac ggaataaggg tggtggataa cctggccgtt
     2161 gggaccaggg aggatctggc agcggccggc gacttcgtag aggtctcgcc ggacgggcct
     2221 ggccccatct ccgccggcgg gcgggtcgag ctcctcgtcg gcgacataca ggatgcgggg
     2281 ctggcgctcg gcgccgccga gggcgccgac gtgatcgtgc acctcgccgc caacacgggg
     2341 gttatgccct ccgtggagga tccccgggct gactgcatga gcaacgttat cggcacgctg
     2401 aactttctgg aggcggcccg ccacaacggc gttgaccgct tcgtgttcgc ctccagcgga
     2461 ggaacggtca tcggggacgc cgagccgccg atacacgagg agatggtcgc ccacccggtg
     2521 gccccctacg gggcgagcaa gctcgccggg gagggctact gctcggctta cttccagacc
     2581 ttcggcatag ggaccgtcgc cctgcgcttc ggcaacgtct acggtccact ctccggccac
     2641 aagaacagcg tggtcgcgcg cttcctcaag cgggcgatgg acggcgaggt gctcgagatc
     2701 tacggcgacg gcacccagac ccgcgacttc atcttcgtgg acgacctgat ccgggccatc
     2761 cgactctcgg ccacgaagga cggcgtcggc ggcgaagtgt tccagatcgc cacgagcgcg
     2821 gaaaccaccg tacaggagct gacggacagg ctcctccccg ccatggccgc cgccggtctg
     2881 gagggcgtag aggtccgtaa cacggacgcc cgccgcgggg aggttcgccg caactacgcc
     2941 gatacctcga aagcacaccg gatgctcggc tggaaggccg aagtgggcct cgacgagggc
     3001 ctcaagcgca ccgttgactg gtttgtgcac gagtaagtag agccgttagc tttcagcttt
     3061 cagcggtcaa ccaggaagct gaaagctgcc cggaggtcgc cgtattgatg ggctgaacag
     3121 gaaggcccta cgcgacggct ttcggcgagt tttagcggct ccggcgatta gcaggttgac
     3181 gaccagagcc aggatcgcgg tgttgtagaa gaacgagaag gccccgtgcg ccaacgtggt
     3241 cctgcgcacc acccgcgacg tcacctctac ctcgggggac gtgaaggtcg tgccgacccc
     3301 gaaggcatag tagtcgaagt cgagcgcgtc ggttcctctc tccggggaat cgaagcccgc
     3361 cgggagacgc ctcatcgccg taatagag
//
LOCUS       DBNGVD010000605         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2357868, whole genome shotgun sequence.
ACCESSION   DBNGVD010000605 DBNGVD010000000
VERSION     DBNGVD010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2357868"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1788
                     /locus_tag="AC16CM_16180"
     CDS             <1..>1788
                     /locus_tag="AC16CM_16180"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIR3141421.1"
                     /translation="GEGTQAKRRAHATVVDHLIPPMTRADTYDDLAKLEQLLDEYYQV
                     ETLDPSKLPAIRVSIWETLRDAELHRDLGVEEQPEEFSDFLTHVDGYLCEIKDLPIRG
                     GLHILGKTPEGEPLRHLVASILRLGAGEIPGLRRAVADAFDLDERFMVENGGTKMEPP
                     QALAERFPGTAATASDVLDRLEEAQQALLLALESCGWEADRADEVCRQVLGFTDQGVK
                     SALRFAADEVVPRLLRTPEEMGNLLSGLEGGYVPAGPSGSPTRGLVNVLPTGRNFYSV
                     DPKALPSRLSWEVGQGLAEDLLRRYLEEEGRYPESVGIVVWGTAAMRTQGDDIAEILA
                     LLGVKPVWNEESRRVTGLEVIPPNELGRPRIDVTVRISGFFRDAFPNLISLLDDAFTT
                     VADLDEPDDMNFVKKHAGEERGSGADERRSTTRIFGSKPGAYGAGLLPLMDARNWRTD
                     EDLAEVYAVWGGYAYGRGLDGVEAREAMEANLRRTEVAVKNIDNREHDLFDSDDYFQY
                     HGGMIAAVRALTGRDPKAYIGDSADPSRVKTRDLSEEARRVFRSRVANPKWIGAMQRH
                     GYKGAFELSATVDYLFGYDATAGVVEDWMY"
BASE COUNT          331 a          582 c          594 g          281 t
ORIGIN      
        1 ggcgagggga cccaggccaa gcgtagggcg cacgccacgg tggttgacca cctgatcccc
       61 ccgatgaccc gcgccgacac ctacgatgac ctggcgaagc tggaacaact gctcgacgag
      121 tactaccagg tggagacgct cgacccatcc aagctgcccg caataagggt cagcatctgg
      181 gagaccctac gtgacgcgga acttcaccgt gacctcggcg tggaagaaca gcccgaagag
      241 ttctcggatt ttctcaccca cgtggatggg tacctctgcg agatcaaaga cctccctatc
      301 cgcggcggcc ttcacatcct cggcaaaacc cccgaaggcg agcccctgcg ccacctcgtt
      361 gcctccatcc tgcgcctcgg tgccggagag atccccggcc tgcgccgcgc cgtggcagac
      421 gcattcgacc tcgacgagag gttcatggtc gagaacggcg gcacgaagat ggaacccccg
      481 caggcgctcg ccgagcgctt ccccggaacg gcggccacgg cctctgatgt gctcgacagg
      541 ctggaggagg cccagcaggc cctgctactc gcgctcgagt cctgcggctg ggaggcggac
      601 cgggcggacg aagtctgcag gcaggttctc gggtttacgg atcaaggcgt taagagcgcc
      661 ctgcgcttcg ctgctgatga ggtcgtgccg cgcctcttgc ggacgccgga ggagatgggg
      721 aacctgctgt ctggtctcga gggcggctat gtaccggccg gaccctccgg ctctccgacg
      781 cgggggctgg tcaacgtgct gcctacgggg cgcaacttct actctgtcga tccgaaggcg
      841 ttgccctccc gactctcgtg ggaggtgggg cagggtctcg cggaggacct gttgcggcgc
      901 tatctcgaag aagaagggcg gtatccggag agcgtcggca tcgtggtctg gggtacggcg
      961 gccatgcgga cgcagggcga cgatatagcc gagatcctcg ccctgctcgg cgtcaaaccc
     1021 gtctggaacg aggagtccag gcgggtgacg gggctggagg tgatcccccc gaacgagcta
     1081 ggccggccga ggatcgacgt caccgtcagg atcagtggct tcttcagaga cgcttttccg
     1141 aacctgatct ccctcctcga cgacgctttt acgactgtag ccgacctcga cgagcccgac
     1201 gacatgaact tcgtgaagaa acacgcgggc gaagagcggg gctcgggcgc cgatgaacgc
     1261 cgctcgacca cgcgcatctt cggctcgaaa ccgggcgcgt acggcgccgg cctgctcccc
     1321 ttgatggacg cccgcaactg gcgcaccgac gaggacctcg ccgaggtcta cgccgtctgg
     1381 ggaggctatg cttacggtag agggttagac ggggtagagg cacgggaggc catggaggcc
     1441 aaccttcgca ggacagaggt ggcggtcaag aacatagaca accgcgagca cgacctcttc
     1501 gactcagacg actacttcca gtaccacggg ggtatgatcg ccgccgtccg cgccctgacg
     1561 ggccgtgacc cgaaggccta catcggagac tccgcggacc catcgagggt caagacgagg
     1621 gacctctccg aggaggcccg ccgcgtgttc cgcagccgcg tggccaaccc gaagtggata
     1681 ggggccatgc agcgccacgg ctacaagggc gccttcgagc tctccgccac cgtggattat
     1741 ctcttcggct acgacgcaac cgccggcgtc gtcgaagact ggatgtac
//
LOCUS       DBNGVD010000606         3484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_5090575, whole genome shotgun sequence.
ACCESSION   DBNGVD010000606 DBNGVD010000000
VERSION     DBNGVD010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_5090575"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16CM_16185"
     CDS             complement(<1..200)
                     /locus_tag="AC16CM_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3141422.1"
                     /translation="MKLYTAEEMSRADAGAQELGIPGGVLMERAGAAMAHVALKRFAP
                     RSAVVVSGGGNNGGDGFVIAREL"
     gene            complement(197..598)
                     /gene="acpS"
                     /locus_tag="AC16CM_16190"
     CDS             complement(197..598)
                     /gene="acpS"
                     /locus_tag="AC16CM_16190"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3141423.1"
                     /translation="MEGFVVPGIGVDVVDVERMQFALERTPKIRRRLFTEAEIAYCEK
                     YRFAERHFAGRWAAKEAVTKALGCGLIQWNGIEVVRLPRQAPTVRITGDKIRRFADMV
                     GVREEDLHISITHSELSAVAVCIVRKEQLSG"
     gene            651..1517
                     /locus_tag="AC16CM_16195"
     CDS             651..1517
                     /locus_tag="AC16CM_16195"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3141424.1"
                     /translation="MDAVIIGNPNSGRAGDAGYLERFAEILRSGGISVEVLNTEHPNH
                     ATELAARAGDRLVVAAGGDGTVNEVLNGLSEGATLAILPLGTANVLARELGLPLDGEA
                     ACRRILEGEVSRVDFGVATDANGVERRFACMAGIGFDAQVVGTVTPRLKRILRGLAFA
                     LTAFRVFFENRFPKLEIVDGDEVHVARFAIVANGHYYGGDYRVTGPGLLTRGEFETVL
                     VERVSILLRPDVFTWIMARKPLNRATKSFTSKEVIARSPGAKVPVQIDGELWGELPMS
                     FRIEPGALKVIR"
     gene            complement(1579..2568)
                     /locus_tag="AC16CM_16200"
     CDS             complement(1579..2568)
                     /locus_tag="AC16CM_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3141425.1"
                     /translation="MRAMILETAGEPLRAVEVAVPEPEEGQVRLRVHACAVCRTDLHV
                     VDGELPDPTLPLILGHQIVGTVDKIGGNVGGFRLGDRVGVPWLGWTDGTCRYCRSGRE
                     NLCENARFTGYQIDGGYAEYTVADARFCLRIPGSYHDLQAAPLLCAGLIGYRSLRFAG
                     DAERLGLYGFGASAHLIIQVAVHQGRRVFAFTSPNDTETQQAARDLGAEWAGSSEERP
                     PEELDAAIIFAPVGSLVPAALGTLARGGTVVCAGIHMSDIPSFSYDHLLWQERSIRSV
                     ANLTRQDGQEFLDLAPEVPVQTSVTPFPLEQANEALDALRNGRLQGSAVLVVD"
     gene            complement(2556..2798)
                     /locus_tag="AC16CM_16205"
     CDS             complement(2556..2798)
                     /locus_tag="AC16CM_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141426.1"
                     /translation="MWLADLLWGIWNGLTAWIVLVVHAFGGWEQFPFYNTARSGNWYD
                     LGFLMGAGSPILGTLRGAVENERRRGRFLGVTSCGR"
     gene            2907..3389
                     /locus_tag="AC16CM_16210"
     CDS             2907..3389
                     /locus_tag="AC16CM_16210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3141427.1"
                     /translation="MIARETPTVRHAARVVLLDESCRVLLARFEYQERVWWAAPGGGL
                     ERAETHEQAARREVREETGLDLTDLGPWIWTREHVFRFAGHLYRQKERYFHATVPDFQ
                     PVPTLLDPEEAAVLRGLRWWTLPELEVTSDEMAPAELPALVRELVENGPRPAPFEVGV
                     "
BASE COUNT          616 a         1122 c         1155 g          591 t
ORIGIN      
        1 agctcgcggg cgataacgaa gccgtccccg ccgttgttac ccccgcccga gacgaccacg
       61 gcgcttcgcg gggcgaagcg tttcagggcg acgtgggcca tggcggcgcc ggcccgttcc
      121 atcaggacgc cgccggggat gcctagttcc tgggcccccg cgtcggcgcg ggacatctct
      181 tccgcggtgt agagcttcaa cccgagagct gctctttgcg cacgatgcag acggccacgg
      241 cggagagctc ggagtgggtt atggagatat ggaggtcttc ctcgcgcacg cccaccatat
      301 cggcgaagcg ccttatcttg tcccccgtaa tgcgaacggt cggggcctgc ctgggaagcc
      361 gcaccacctc gatgccgttc cactggatca ggccgcaccc aagggccttg gtcacggcct
      421 ccttggcggc ccagcgtccc gcgaagtggc gctctgcgaa gcggtatttc tcgcagtagg
      481 cgatctcggc ctccgtgaag aggcggcggc ggattttggg ggtccgctcg agggcgaact
      541 gcatccgctc gacgtcgacg acgtctaccc cgatccctgg tacgacgaat ccttccacgg
      601 cgcatatgtt acggcagaat ggcccaggac aaaagggcta tagtatcgga atggacgctg
      661 tgatcatcgg gaacccgaac tcggggcggg ctggagacgc gggttacctg gagcgtttcg
      721 cggagatttt gcgctcagga ggcatctcgg tggaggtgct caacaccgag catcccaacc
      781 acgccaccga gctcgcggcc cgcgccggcg atcgcctggt ggtggccgcg ggcggcgacg
      841 ggacggtgaa cgaggtcctg aacgggctgt ccgagggggc cacgttggcc atcctgccgc
      901 tcggcacggc gaacgtgctg gccagagagc tgggcctgcc gctggacggg gaggccgcct
      961 gccggcgcat cctggaggga gaggtctccc gggttgactt cggcgtcgcc acggacgcaa
     1021 acggtgtgga gcgccgcttc gcctgcatgg ccggcatcgg cttcgacgcg caggtggtag
     1081 gcaccgtgac accgcgcctc aagcgcatcc tgcgcgggct ggccttcgcc ctgacagcgt
     1141 tcagggtgtt cttcgagaac aggttcccca aactggagat agtagacggg gacgaggtcc
     1201 acgtcgcgag gttcgctatc gtcgccaacg gccactacta cggcggggat tacagggtga
     1261 ccggccccgg cctgctgacc aggggcgagt tcgagaccgt gctggtcgaa cgcgtaagca
     1321 tcctcttgag gcccgacgta ttcacctgga tcatggcccg caagcccctg aaccgcgcga
     1381 caaagtcgtt tacctccaaa gaggtgatcg ccaggtctcc cggcgcgaag gttcccgtgc
     1441 aaatcgacgg cgagctctgg ggagaactgc ccatgtcgtt ccgcatcgag ccaggcgccc
     1501 tgaaggtgat caggtagcca ggcagcacgc cggggaccgc gccgcaggga ctacttcacc
     1561 ctcgccaagc gtaacgggct aatcgaccac gagcacggcg gatccctgca ggcggccgtt
     1621 gcgcagggcg tccagggcct cgttggcctg ttccaggggg aacggggtga cgctggtctg
     1681 gacgggcact tcgggcgcga ggtccagaaa ctcctgcccg tcctgtcggg tgaggttggc
     1741 cacggagcgt atcgagcgct cttgccagag aaggtggtcg taggagaacg aggggatgtc
     1801 gctcatgtgt atgccggcgc agacgacggt tccacccctg gccagggttc ctagtgccgc
     1861 cgggaccagt gatcctacag gggcgaagat tatcgcagcg tccagttcct ccggcggcct
     1921 ctcttctgag cttccggccc actcggcacc gaggtctctt gcggcctgct gggtctccgt
     1981 gtcgttcggg ctggtgaagg cgaagacgcg ccggccctga tgaaccgcca cctggatgat
     2041 gagatgggcc gaggcgccga aaccgtagag gccaagcctc tcagcgtcac cggcgaagcg
     2101 cagggaacgg tacccgatca ggccggcgca caacagcggc gcggcctgca ggtcgtgata
     2161 ggaaccaggg atgcggaggc aaaaacgagc gtcggcgacc gtgtactcag cgtagccgcc
     2221 atcgatctgg tacccggtaa acctggcatt ctcacagagg ttctcgcgtc cggagcggca
     2281 gtagcggcac gtaccgtcgg tccaccccag ccacggcacc cccaccctgt ctcccaacct
     2341 gaaaccaccg acgttgccgc cgatcttatc cacggtgccg acgatctggt ggccaaggat
     2401 caggggcagc gtagggtcgg gcaactcccc gtcgactacg tgaagatccg tcctgcaaac
     2461 ggcgcaggcg tgtaccctta gtcgcacctg accttcctcc ggctcgggaa cggccacctc
     2521 cacagccctc agcggctctc ctgctgtctc aaggatcatc gcccgcacga ggtcaccccc
     2581 agaaaacgcc cgcgtcgtct ctcattctcg acggccccac gcagggtacc taggatgggt
     2641 gacccggcgc ccatcaggaa cccgaggtcg taccagtttc cgctccttgc cgtgttgtag
     2701 aaggggaact gttcccatcc gccgaacgcg tgcactacaa gcacgatcca ggccgtcagg
     2761 ccgttccaga ttccccagag caagtcagcc aaccacataa cggtcctcct cgaggtcgcc
     2821 tgccggaact tgtcccgctc tccacatcgt acagagtact gcaagtcgtc ccgttttcgc
     2881 ggccacggct tcggtaaact cgaaaaatga ttgccaggga gacgcctacc gttcgtcacg
     2941 ccgcccgagt ggtgctgctt gatgagagct gcagggtact gctggcaagg ttcgagtacc
     3001 aggaaagggt gtggtgggcc gctccgggag gagggctcga gcgcgcggag acgcacgagc
     3061 aagccgcacg gcgcgaggtc agagaggaaa ccggcctgga cctcacggat ctcggaccct
     3121 ggatctggac ccgggagcac gtcttccgct tcgcgggaca cctctacagg cagaaggagc
     3181 gatatttcca cgccacggtt cccgacttcc aacccgttcc taccctgctc gacccagagg
     3241 aagccgctgt gctgcgaggg ctccggtggt ggacgctccc cgaactggag gtgacgtcgg
     3301 atgagatggc gccggcagag ctgcccgcgt tggtgaggga gcttgtggaa aacggtcctc
     3361 ggccggcacc tttcgaggtg ggagtttgag ggggattcgc gaagcatccc tacattattc
     3421 gaccgttacg ctcttggcga ggttgcgggg tttgtccacg tccaggccgc ggtcggtggc
     3481 caca
//
LOCUS       DBNGVD010000607         2596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3997512, whole genome shotgun sequence.
ACCESSION   DBNGVD010000607 DBNGVD010000000
VERSION     DBNGVD010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3997512"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1270
                     /locus_tag="AC16CM_16215"
     CDS             77..1270
                     /locus_tag="AC16CM_16215"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3141428.1"
                     /translation="MATTDHVRAVAERIKRAHDSGLGLVVTVSAMGKQTDRLLRLAGE
                     VSRDPSPREIDQLLSTGELQSVALLAMALHDRGVAATSLTGPQAGMKISGRYGSGVIS
                     GIQPDRLRGLLGEGQVAIVAGFQGVNALGDIMTLGRGGSDTTAVALAAALGAERCEIF
                     TDVDGVYTADPRIVPGARRIPVILSSEMAEMSWRGARVMHPRAVELGALYGVEIHVKS
                     SFEEGPGTIITGGNDLEHLETRETVAGIVHDLDVSRITLTGIKTGPGTMSRVFTPLAE
                     AGISVDVIVESAPKEGASDVAFTVRRSDFGEALGIAGGVAESLDGRVEGEEELGKVSV
                     VGTGMLNRPGYAARMFATLGEAGIPIRLVSTSEIQVTCVLPAGRVQEAVRVLHRDFEL
                     EERDV"
     gene            1263..2156
                     /gene="dapA"
                     /locus_tag="AC16CM_16220"
     CDS             1263..2156
                     /gene="dapA"
                     /locus_tag="AC16CM_16220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3141429.1"
                     /translation="MFSGAFTALVTPFRNGEVDVEALEGMVEFQIQHGIHGLVPCGTT
                     GETPAMSELEDRLVIGTVVRVAAGRVPVVAGTGSNSTDMAIKYTKMAEEEGADGSLQV
                     APYYNKPTQEGLYRHFTAVAESTSLPILLYNIPGRTAVTISAETMARLADVPNIVGVK
                     ESTLSMNMVSDVKSLCGEEFALLSGDDPMTLPLMALGGHGVVSVASNVAPAAVSDMVN
                     ALSGGDWERGRELHYELLPLFRALFVETNPIPVKTAASILGLCSDELRLPMVPLQGDN
                     LDTLRRTMEESNHLLPTPEEV"
     gene            2153..>2596
                     /locus_tag="AC16CM_16225"
     CDS             2153..>2596
                     /locus_tag="AC16CM_16225"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013293.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016726 - oxidoreductase activity, acting
                     on CH or CH2 groups, NAD or NADP as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3141430.1"
                     /translation="MTRVTIIGAAGRMGRELCCAAIETEGIELAGGTVEPGAPELGAD
                     LGELCGSGKIGVTATEDPPERAGVLIEFTTPAATVAHLSYERPHVIGTTGLSEYQLSE
                     VEKAAESVPVVLAPNMSIGVNLLRDVVRELSAKLGENYDIELVETH"
BASE COUNT          450 a          832 c          897 g          417 t
ORIGIN      
        1 cttatccttc cagaagcacg atcttcggag ggtttcgctc ttggcaatca tcgtccagaa
       61 gtacggcggg agctcggtgg cgacgacgga ccacgtcagg gccgtcgccg agaggatcaa
      121 acgcgcccac gattcggggc tcgggcttgt cgttacggtc tcggcgatgg gcaagcagac
      181 agaccggctc ctcaggctcg ctggcgaggt cagccgcgac ccttctccgc gcgagatcga
      241 ccagcttctc tccacgggcg agcttcagtc tgtggcgctg ctggcgatgg ccctgcacga
      301 caggggcgtc gcggccacct cgctcaccgg cccgcaggcc ggcatgaaga tctcgggccg
      361 ttacggttct ggggtcatct cgggtattca accagaccgg ttgcgtggcc tgctcggcga
      421 ggggcaggtc gccatcgtgg ccgggttcca gggcgtgaac gcgttggggg acattatgac
      481 gctcgggcgc ggggggtctg ataccacggc ggtggcgctc gcggcggcgc tcggggccga
      541 gcggtgtgag atctttacgg acgtggacgg cgtgtacacg gccgacccgc gcatagttcc
      601 aggggcgcgg cgcatacccg tcatcctctc ctccgagatg gccgagatgt cgtggcgggg
      661 cgcgagggtc atgcacccgc gggccgtcga gctcggggcc ctttacggcg tcgagatcca
      721 cgtcaagagc tccttcgagg aggggcctgg tacgatcatc acgggaggca acgatttgga
      781 acacctggag acacgcgaga cggtcgccgg catcgtccac gacctggacg tctcgcgcat
      841 aaccctgacg ggcataaaga ccggcccggg caccatgagc cgcgtattca cacccctggc
      901 cgaggcgggt atctccgtgg acgtcatcgt ggagagcgcg cccaaagagg gcgcctcgga
      961 cgtggccttc accgtccgcc gctccgactt cggggaggcc ctgggtatcg ccggcggggt
     1021 cgccgaatcc ctcgacgggc gggtcgaagg cgaggaggag cttggcaagg tctccgtggt
     1081 cgggacgggg atgctcaacc gtcccggcta cgcggcgagg atgttcgcca cgctcgggga
     1141 ggccggcatc cccataaggc tagtctccac ctccgagata caggttactt gcgtgttgcc
     1201 cgccgggcgg gtacaggagg cggtaagggt tttgcacagg gacttcgagc tggaggagag
     1261 agatgtttag tggcgcgttt acggccctcg ttacgccttt tcggaacggg gaggtggacg
     1321 tggaggccct cgaagggatg gtcgagttcc agatccagca cggcatccac ggcctcgtgc
     1381 cctgcggaac cacgggcgag accccggcca tgagcgagct tgaagacagg ctcgttatcg
     1441 gcaccgtggt cagggttgcg gccgggcggg ttcccgtcgt ggccggcacg ggctccaaca
     1501 gcacggatat ggcgatcaag tacacgaaga tggccgaaga ggagggcgcg gacggctcgc
     1561 tgcaggtggc gccctactac aacaagccga cccaggaggg gctctacagg cacttcaccg
     1621 ccgtcgccga gagcaccagc cttcccatcc ttctctacaa cattccgggc cggacggccg
     1681 tgaccatctc cgccgagacc atggcccgcc tcgccgacgt gccgaacatc gtcggggtca
     1741 aggaatctac tctctccatg aacatggtga gcgacgtgaa gagcctgtgc ggcgaggagt
     1801 tcgccctgct ctccggtgac gacccgatga cgctaccgct catggcgctc ggcgggcacg
     1861 gtgtggtctc cgtcgcctcc aacgtggccc ccgcggcggt ctccgacatg gtcaacgccc
     1921 tctccggggg agactgggag cggggacgcg agctccacta cgagctgctg ccgctcttca
     1981 gggccctctt cgtggagaca aacccaatac cggtcaagac ggcggcctcc atcctgggac
     2041 tgtgctcgga cgagctgcgg ctgccgatgg tccccctgca aggcgataac ctcgacaccc
     2101 tcaggcgcac catggaggag tcgaaccacc tcctcccgac ccccgaggaa gtgtgacccg
     2161 cgtaaccata atcggggcgg ctggccgcat gggccgcgag ctgtgctgtg ccgccatcga
     2221 gacggaaggg atagagctgg ccggcggcac cgttgaaccc ggagcccccg agcttggcgc
     2281 cgacctcggg gaactgtgcg gctcgggcaa gataggcgta accgcgaccg aggaccctcc
     2341 ggagcgggca ggagtcctga tcgagttcac caccccggcg gcgacggtgg cccacctctc
     2401 ctacgaacgg ccccacgtca taggcacgac gggcctgtct gaataccagc tgtcggaggt
     2461 cgagaaggcc gccgaaagcg ttcccgtagt gctcgcgccg aacatgagca tcggggtaaa
     2521 cctgctgcgc gacgtggtac gcgagctctc ggcgaagctc ggcgaaaact acgacatcga
     2581 gctggtagag acgcac
//
LOCUS       DBNGVD010000608         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_717436, whole genome shotgun sequence.
ACCESSION   DBNGVD010000608 DBNGVD010000000
VERSION     DBNGVD010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_717436"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16CM_16230"
     CDS             <1..784
                     /locus_tag="AC16CM_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3141431.1"
                     /translation="VPLLTHADEIRLANGIARGCKRSKDRLVEANLRLVVSIAKKYRN
                     RGVSFLDLIQEGNLGLIRAAEKFDPSKGFKFSTYATWWIRQAITRAIADKGRTIRIPV
                     HMVEKVNKYHRTHRRMIQTLGREPSEEEIALELGVFVEEIQRLQEISQRSISLATPVG
                     DDDSSELGDFLEDAGSVTPTDAVSESLLKAHLREALDELPERERQIIELRFGMKDDRP
                     RTLEEVGREFDITRERVRQIQMKTLNLLREQRRTQNLREYLH"
     gene            792..1577
                     /locus_tag="AC16CM_16235"
     CDS             792..1577
                     /locus_tag="AC16CM_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3141432.1"
                     /translation="MDSTQDLHAFVCEVARRAGDLQLARYQNPGEVREKAPKDFVTEV
                     DLLCEELMVQSIEERFPDDAILSEERGGAVSEEKRSWLLDPVDGTANFMKANPMFCAC
                     VTVMEGGVVTHAAVAAPRIGDLYHASAGGGAFRETRGERFPLRVSDTKKLEYAVAGAD
                     LSLRSGETRDTGADRVLSDAWQLRALGSAGIRGAWLAAGYLDVSIGTRNTAWDYAPTV
                     LLVSEAGGRATDLSGEPWSLSAEGLIATNGILHDEVLETLHTA"
     gene            complement(1605..>2458)
                     /locus_tag="AC16CM_16240"
     CDS             complement(1605..>2458)
                     /locus_tag="AC16CM_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141433.1"
                     /translation="CGALLVVTSQALLSDRHGNFGAVAVTESNITASACAIAAPLLVG
                     AFAAAGLGWRAAFAVPIVALVVLALAFFFRPPHLPRTTAKDEDRPAGLALPPRYWALW
                     ALVALGVASEWCVAYWGADFLADGTDLGRAAAATSLTTFFVAMMVGRIASSRLARSVP
                     PALLLATTLCLALLGFPLFWLSPGSVLTLIGLFLTGLGIGGVYPLGVSAAIASAPGNS
                     DAAAARLSIGGGGAILTAPFILGALADRIGIATAFGIVVPMLLAALTLALVALRRDGR
                     KADKAAP"
BASE COUNT          479 a          806 c          834 g          339 t
ORIGIN      
        1 ggtgcctctc ctgacccacg ccgacgagat ccggctcgcc aacggcattg cccgcgggtg
       61 caagaggtcc aaggaccgtc tggtcgaggc aaacctcagg ctcgtcgtct ctatcgccaa
      121 aaagtaccgt aaccgcgggg tctctttcct ggacctcatc caggaaggaa acctcggcct
      181 catcagggcg gctgagaagt tcgatccctc caaaggcttc aaattctcga cgtacgccac
      241 gtggtggatc cgccaggcta taaccagggc catcgccgac aagggacgca ccatccgcat
      301 ccccgttcac atggtcgaga aggtcaacaa gtaccacagg acgcaccggc gcatgatcca
      361 gaccctcgga cgcgagcctt cggaagaaga gatcgcgctc gagcttgggg tcttcgtgga
      421 ggagatccag cgcctgcagg agatcagcca gcgctccatc agcctggcga ccccggttgg
      481 cgacgacgat tcttcggagc ttggcgactt tctggaggat gccgggtcgg taacccccac
      541 ggacgccgtc tccgagtcgt tgctgaaagc ccacctgcgt gaggcgctcg acgagctccc
      601 ggagagggag cgccagatca tcgagctgcg tttcgggatg aaggacgacc gtcccaggac
      661 gctcgaagag gtcggccgcg agttcgacat cacgcgcgag agggtgcgcc agatccagat
      721 gaagaccctg aacctgctgc gcgagcagcg ccgcacccag aacctccgcg agtacctcca
      781 ctaggaacgt cctggattcc acgcaagacc tccacgcgtt cgtctgcgag gtcgcccgca
      841 gggcgggtga cctccagctc gcccgctacc agaaccccgg cgaggtaagg gagaaagccc
      901 cgaaagactt cgtgacggag gtcgaccttc tgtgcgaaga gctgatggtg caatccatcg
      961 aggagcgctt ccccgacgac gccatcctct cggaggagcg tggcggggcc gtctcagagg
     1021 agaagagatc ctggctcctc gaccccgtgg acgggaccgc caacttcatg aaggcgaacc
     1081 cgatgttctg cgcctgcgta acggtgatgg aaggcggcgt cgtgacccac gcggcagtcg
     1141 ccgcgccccg cattggtgac ctctaccacg ccagcgccgg cggcggggct ttccgggaga
     1201 cgcgcggcga aaggtttcct ctgcgcgtca gcgacacgaa gaaactcgag tacgccgtag
     1261 ccggcgccga cctctcgctg cgaagcggcg agaccaggga tacgggcgcg gacagggtac
     1321 tctcagatgc ctggcaactc agggccctcg gcagcgctgg catccgaggc gcctggctcg
     1381 cggcgggcta cctcgacgtc tcgatcggca cccgcaacac ggcctgggat tacgccccga
     1441 cggtcctact cgtctcagag gccgggggaa gggccacgga cctctccggc gagccgtgga
     1501 gcctctccgc ggaaggcctc atcgccacca acggtatcct gcacgacgag gttctggaga
     1561 cgctacacac ggcctgatgt aggccgcttc ggtacgtacg tcggtcaggg tgcggccttg
     1621 tcggccttcc ggccatcgcg tctcagggcg acgagcgcca gcgtcagggc cgcgaggagc
     1681 attgggacca ctatgccgaa ggccgtggct atgccgatgc ggtcggcaag cgcgcccagt
     1741 atgaaggggg ccgtgagtat ggcccctccc cctccgatag agagcctggc ggcggccgcg
     1801 tcgctatttc ccggcgccga ggcgatagcc gccgagaccc caagcgggta gacgccgcca
     1861 ataccaagcc cggtcaagaa gaggccgatg agggtcagga cgcttccagg ggagagccag
     1921 aagagcggaa acccaaggag cgccaggcag agcgtcgtgg cgaggaggag cgccgggggc
     1981 accgaacggg caaggcggct gctcgcgatc ctgccaacca tcatcgccac gaagaaggtg
     2041 gtaagtgagg tggcggcggc cgcccggccg aggtcggtgc cgtcggccag aaagtcggcc
     2101 ccccagtagg ccacgcacca ctccgaggcc acgccaagcg ccacgagcgc ccagagcgcc
     2161 cagtagcgtg gcggcagggc caggccggcc ggacggtcct cgtccttcgc cgtcgtccgc
     2221 ggcagatggg gtggcctgaa aaagaacgcc agggcgagca caacgagggc tacgatgggt
     2281 acggcgaagg cggcccgcca gccgagaccg gcggcggcga aggctccgac gaggagcggg
     2341 gccgcgatgg cacacgcgct agcggtgatg ttcgactcgg tgacggcaac ggcgccaaag
     2401 ttcccatgcc tgtcggatag cagggcctgg gaggtaacga ccagcaaggc gccgcaga
//
LOCUS       DBNGVD010000609         3618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3999187, whole genome shotgun sequence.
ACCESSION   DBNGVD010000609 DBNGVD010000000
VERSION     DBNGVD010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3999187"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16CM_16245"
     CDS             <1..276
                     /locus_tag="AC16CM_16245"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017602621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3141434.1"
                     /translation="RLAEAPLAEEVATALDATDKDVEIGGAGLAAQAFELGLVDELRM
                     FRYPVVVGGGTPFLPPVTEDVRLDLIETRTFDSRVIYERYRRVGGVL"
     gene            850..1971
                     /locus_tag="AC16CM_16250"
     CDS             850..1971
                     /locus_tag="AC16CM_16250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3141435.1"
                     /translation="MTSNRQGADYPASTASVEALAEERTRSGDVPGLSVAVAKSGRVV
                     WARGFGFSDLATSTPAAAQTSYLWFSMTKIATATAVMRLAEEGGLDLDAPADEYYPAF
                     KVVSQPTPVSVRHLLNHSSGLANPVPIRWVYPAAGPVPDRRAFVERLLGRHRRLRFVP
                     GETARYSNLGYLVLGEVVSEVSGIDYEHYVCQEILSPLGLGRTGFAYPHDAGNTVATG
                     YQRLWRPLTPLFKAALPRGIVGARHGRYVSFVPFYVKGPAYGGLIGSVEEVARFLLLH
                     LNGGEVDGTRLVSEESVAEMQRLIPRGGKRDFGLGWFRSREASDKGPAFVEHLGGGAG
                     FWNVMRVYPEESLGVVIMGNSTSYDHESILDAIIGTGWT"
     gene            2021..>3618
                     /locus_tag="AC16CM_16255"
     CDS             2021..>3618
                     /locus_tag="AC16CM_16255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3141436.1"
                     /translation="MNSMPEPKVCPVILAVDDGREEFELVERELRKRYEADYLVVCEG
                     SAEDGLERLRRFEAAGQDLALVLAGLRKSGMTSVDFLVHVRDSFPLAKRLLLLDPMDG
                     ETRDLLPQAMALGLIDYFEFKPGVPPNERFHRVVTELLEEWVRPYHSEANALVSVVGE
                     GRLRRSHEIRTLLERYGVPHAFHAVDTDEGRGILEKTGRSEGQLPVIVLPGGRVLVDP
                     ADSEVADAFVPADVHPSRQTVDVVVVGGGPAGLSAGVYAASEGLSTVIVEGEAIGGQA
                     GASSLIRNYLGFARGVSGQQLTYQAFQQATLFGASFRLLRQATALRSEGEELVVTLSD
                     GTEVAGRAVIVATGASYRRLDIPELDDLIGAGVFYGAASSEARALEGREVYVVGGANS
                     AGQAALHLSRYASRVTLVVRGGSLTAGMSDYLIRGIDATNNVEVCLNTRVVGGGGEGR
                     LERVVLEDDALGRSETVSAAALFVLIGAEPRTGWLPADVLRDERGFLLTGQQVLSANL
                     PRSRRSPERSPMLLETSMPGVFAAG"
BASE COUNT          587 a         1141 c         1255 g          635 t
ORIGIN      
        1 cgcctcgccg aagcgccact ggccgaggag gtcgccacgg cgctcgatgc taccgacaag
       61 gacgtggaga tcggcggcgc cggcctggcc gcgcaggcgt tcgagctagg cctcgtcgac
      121 gagctgcgca tgttccgcta tccggtcgtc gtcggtggcg gcacgccgtt cctgccgccc
      181 gtcaccgaag acgttcgact ggacctgatc gagaccagga cgttcgactc acgcgtgatc
      241 tacgagcgct acaggcgagt cggaggagtt ctctgagccg ttccccaaac gctggcgggg
      301 tggtctcgca cgagagcgcg ctcgagctct gggacctttc ggacgtgatc ccaaaagcgg
      361 tgcacgtctc gctcccgcgc gtcgaacagc gaagcggcgc aacgaccttc cgtgccagct
      421 tccatgaggg ccgggggccg tccctcgagg acggtctgcg gtccgagcca cggagcgacg
      481 ctgaccacgg ccccttgcgt cgagaggtta gctacttcgg tgcgattcgt atcatgtaga
      541 ggcggggggc gcggagcttc gttggagcga tctgcgactc gactctagtg cctaccacca
      601 cacgtcgcgg tggtgcgtga aaacaagcgg gtgtgcgctc tagagggtta gtaccaaacg
      661 ttgtcgagat ccctcaggtg tgttcccaag aacctcactg tagcttcgac ctggctcctc
      721 tgtgcaggtg gccttcggcg ctgccggcgg cgtacagggc gagccgcgcg tatatccgct
      781 acgctaccga ggcacgcgcg tacgacgcgc gtgcgagatg gcggccgaca tctgacggag
      841 gcgagcgcgt tgacgagcaa ccggcaaggt gcggactacc ctgcaagcac tgcatcggtt
      901 gaggcgctcg ccgaagagag aacgcgctcg ggagacgtgc cgggcttgtc ggtcgccgtg
      961 gcgaagagcg gccgcgtcgt ctgggcgagg ggcttcgggt tttcagacct cgccacttcg
     1021 accccggctg ccgcccagac gagttacctg tggttttcga tgaccaagat tgctacagcc
     1081 acagcggtga tgcggctcgc cgaggaggga gggctcgacc tggatgcccc tgccgacgag
     1141 tactacccag ccttcaaggt cgtctcccag ccgactccag tgagcgttcg gcacctcctt
     1201 aaccatagct ccggactcgc caaccccgta cctatcaggt gggtttatcc ggccgcgggc
     1261 cccgtccccg accggcgagc cttcgtcgag cgcctactcg gcaggcatcg caggctcagg
     1321 ttcgtacccg gcgagaccgc caggtattcg aacctgggct acctggtgct gggggaggtt
     1381 gtctccgaag tctccggcat agactacgag cattacgtgt gccaggagat tctctctccg
     1441 ctagggttgg gccgcacagg gttcgcctac ccgcacgacg cggggaatac ggtggccacc
     1501 ggctaccagc gcttgtggag acccctgact cccctcttca aggccgcgtt gcccagaggc
     1561 atagttggcg ccagacacgg gaggtacgtt tcgttcgttc ccttctacgt caagggacca
     1621 gcttacggcg ggctgatcgg gagcgtagag gaggtcgccc ggttcctgct actccacctg
     1681 aacggtgggg aggtcgacgg cacgcggctc gtttcggagg aatcggtggc cgagatgcag
     1741 aggctcatac ccagaggagg caagcgagac ttcgggctgg gctggttccg ctcgcgcgag
     1801 gccagcgaca aaggtcccgc ctttgtcgag catcttggtg gcggagccgg cttctggaac
     1861 gtgatgcgcg tttaccccga ggaatcattg ggggtggtga tcatgggcaa ctccactagc
     1921 tacgaccacg agtccatcct cgatgccata atcggaaccg ggtggacgtg accttctgcg
     1981 tcaggagatt ccctttctac gccgcaagcg gggtgtcccg atgaacagca tgccggaacc
     2041 gaaggtttgc ccggttatcc tggccgtgga cgatgggcgc gaggaattcg agctggtcga
     2101 gcgcgaactt cgcaagcgct acgaggctga ctaccttgtc gtgtgtgaag gctcagccga
     2161 ggacgggctc gagcggttgc ggcgtttcga ggctgccggg caggacctcg ccctggtgct
     2221 cgcgggcctg cgaaagtcag ggatgaccag cgtggacttc ctcgtccacg ttcgcgactc
     2281 cttcccgctc gccaagcgcc tgctcctgct cgacccgatg gacggggaaa cccgagacct
     2341 gctcccgcag gcgatggcgc tcgggctgat cgactacttc gagttcaagc ctggagtgcc
     2401 gccgaacgag cggtttcacc gcgtcgtgac cgagctcctg gaggagtggg tgaggcccta
     2461 ccactccgag gcgaacgccc tggtcagcgt ggtcggcgaa gggcgtttgc gccgatcgca
     2521 cgagatcagg accctgctcg aacgctacgg ggtgccccac gcgttccacg cggtcgacac
     2581 cgacgagggt cgggggattc ttgaaaagac cggccgcagc gaggggcagc ttcccgtcat
     2641 cgtgctgccc ggcgggcggg ttctcgtaga cccggccgac agcgaggtag ccgatgcctt
     2701 cgtgcccgcc gacgtgcacc cgtcccggca gactgtcgac gtggtcgtgg ttggtggagg
     2761 gccggcgggc ctgtccgccg gcgtgtatgc cgcttcggag ggcctctcga ccgtgatcgt
     2821 cgagggggaa gccataggcg gccaggccgg cgcaagctcg ctgatccgca actacctggg
     2881 gttcgcccgc ggggtcagcg gccagcaatt gacctaccag gccttccagc aggccaccct
     2941 gttcggcgcg agcttccgcc tcctgcggca ggccacggcc ttgcgcagcg aaggggagga
     3001 gctggttgtg accctctcgg atggcaccga ggtggcaggc cgcgcggtca tcgtggccac
     3061 gggagcctcc tacaggcgtc tggacattcc ggaactcgat gacctgatcg gggctggcgt
     3121 cttctacggg gccgcatcga gcgaagcgcg agccctggaa ggccgggagg tgtacgtggt
     3181 tggcggggcc aactccgccg ggcaggcggc gctccacctg tccaggtacg cttcgagggt
     3241 gacgctcgtc gtgcgcggcg gttcgctgac ggcaggcatg tccgactacc tgatcagggg
     3301 gatcgacgcc acgaacaacg tggaagtctg cctgaataca cgggtcgtag ggggcggagg
     3361 cgaagggcgg ttggagcggg tggtcctaga agacgacgcc cttggacggt cggagaccgt
     3421 tagcgccgca gccctgttcg tccttatcgg ggcagagccg cgcaccgggt ggcttcccgc
     3481 agatgttctg cgagacgagc gtgggttcct gctcaccggt cagcaagtgc tgagcgccaa
     3541 ccttccgcga tcccgacggt ccccggagcg ttcgccgatg ctgctggaga cgagcatgcc
     3601 cggcgtcttc gccgccgg
//
LOCUS       DBNGVD010000610         3230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4910141, whole genome shotgun sequence.
ACCESSION   DBNGVD010000610 DBNGVD010000000
VERSION     DBNGVD010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4910141"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..859
                     /locus_tag="AC16CM_16260"
     CDS             101..859
                     /locus_tag="AC16CM_16260"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3141437.1"
                     /translation="MRLIGAGFGRTGTTSLKAALERLGLDPCYHMTEVFAHPDHADFW
                     VRAWHGKPVDWDGVIGGYEATVDWPACTFYETLMERYPEAKVLLSVRDPERWYESTRG
                     TIYELTRLTEGSTISRLIFGVVGLLVFGGFTGRRSSLANDLIWEGTFDGKFEDRAYAM
                     GVFERHNEEVKRRVPRDRLLVYEVSEGWGPLCEFLCVPVPDEPFPRLNDTAQMKRGMR
                     AIKALSVALPAALALTVAAALVLLLRRAGDQSSG"
     gene            complement(821..1432)
                     /locus_tag="AC16CM_16265"
     CDS             complement(821..1432)
                     /locus_tag="AC16CM_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3141438.1"
                     /translation="MARSKEFEPIEALDAAMELFWRKGYEAASMRELLDAMGIGRGSF
                     YDTFGDKHSLFLSALDRFQEVRASWIEEALRGEGLGGIEDVFHRSLNGLYQYRPPRGC
                     LLANSAVELAPHDPEVAARISAYIWRTEDAFQAALERARDGGEIPSNSDPRALARFLV
                     NTLHGLRVLARAGADRGTLEDTVRVALGALRSAGGLVSGAPEE"
     gene            complement(1436..2689)
                     /locus_tag="AC16CM_16270"
     CDS             complement(1436..2689)
                     /locus_tag="AC16CM_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3141439.1"
                     /translation="MEVGRVRWWRSTAFVVGCGCTIALITYGLRTSFGLFATPIGEGR
                     GWSPEVFALAIAIQNLVWGIGQPFAGGVADRFGSGRVLAAGGVVYAVGVALMALSPTP
                     LAFNLTGGVLVGLGLSGASFTIVIAALGRLVPAERSSQAMGLATAAGSLGQFVFAPLG
                     QAFLTDYGWQTTLLLLAGSMVVVPFLAAALRGGGRQEGTGVRALPARDAVREAFGHGS
                     YLLLVAGFFVCGFHVAFITTFLPAHVAAVAGHSHDGMHMAGPGIAAWALALIGLFNIV
                     GSYGSGVLGARHSKRQLLAGIYLARAAVIAVFITLPPTPTVVLVFAAAMGILWLSTVP
                     LTSGLVAVMFGTGNLGTLFGVVFLSHQVGAFLGVWLGGMAFEQTGSFLPIWWAGIALA
                     IIAAGLHWPIVERPAPSYAAAQAEG"
     gene            2879..>3230
                     /locus_tag="AC16CM_16275"
     CDS             2879..>3230
                     /locus_tag="AC16CM_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3141440.1"
                     /translation="MAETWEVVVQGEVPATMRDGTVLMSNVYRPAGGGPYPVLLSRHP
                     YGKDISSGTSMLDPIKAAKAGYIVVVQDVRGRYRSGGSFISFVSEYEDGYDSVEWAAS
                     LPGSDGSVGMHGLSY"
BASE COUNT          603 a         1101 c         1052 g          474 t
ORIGIN      
        1 gcctacgagt tcgtggaggc cctacccatg acggcaacag gcaagttcca gaagctgaag
       61 cggcgcgagc agttcgaggg ttactcgttc gaaggctagc gtgaggctca tcggcgcagg
      121 cttcggccgg acgggcacga cctccctgaa agccgcgctg gagaggctcg gcctcgaccc
      181 ttgctaccac atgaccgagg tgttcgcgca ccccgatcac gcggacttct gggtccgcgc
      241 ctggcacggg aagccggtgg actgggacgg ggtcatcggt ggctacgagg ccacggtgga
      301 ctggcccgcg tgtacgttct acgagacgct gatggaacgg tatcccgagg ccaaagtgct
      361 cctcagcgtg cgcgacccgg aacgctggta cgaaagcacc cgcggcacca tctacgagct
      421 gaccaggctg accgagggct ccacgatctc aaggctcatc ttcggggtgg ttggcctcct
      481 ggtgttcggc ggcttcaccg gccgcaggag cagcctggcc aacgacctga tctgggaggg
      541 aaccttcgac ggcaagttcg aggacagagc ctacgctatg ggggtcttcg agcggcacaa
      601 cgaagaggtg aaacgccgcg tccccaggga ccgactcctg gtctacgagg tgagcgaggg
      661 ctggggtccg ctctgcgagt tcctctgcgt gcccgtgccg gacgagccgt ttccccgcct
      721 caacgacacg gcccagatga aacgcggcat gagagctata aaggcgctct ccgtggccct
      781 gcccgcggcg ctggcgctca ccgtggccgc cgcgctcgtc ctactcctca ggcgcgccgg
      841 agaccagtcc tccggctgac cgaagcgccc cgagggcgac gcgaacggtg tcctcaagcg
      901 ttccgcggtc ggcgcctgcc ctggccagca cccggaggcc gtgcaacgtg ttgaccagaa
      961 acctggccag agccctgggg tcgctgttgg aaggtatctc gcccccgtcc ctggctctct
     1021 ccagggcggc ctggaaggca tcctcggtgc gccagatgta agcggagatt cttgccgcta
     1081 cttcggggtc gtgcggcgcc agctccaccg ccgagttggc gaggaggcaa ccccggggcg
     1141 gccggtactg gtatagcccg ttcagggatc tgtggaacac gtcttctatg ccccccagcc
     1201 cttccccgcg gagcgcctcc tcgatccagg aagcccttac ctcctggaaa cggtccaggg
     1261 cggagagaaa gagggagtgc ttgtccccga aggtatcgta gaagctaccc cggccgatgc
     1321 ccatcgcatc gagcagctcg cgcatcgagg ctgcctcata ccccttgcgc cagaacagct
     1381 ccatcgccgc gtcgagcgcc tcgatgggct cgaattcctt ggatcgcgcc accctctacc
     1441 cctcggcctg ggccgcggcg tatgacggag ccggacgctc gacaatgggc cagtgcaggc
     1501 ccgcggcgat gatggccagg gcgatgccag cccaccatat cggcaggaac gagccggtct
     1561 gctcgaaagc catcccacca agccagaccc cgagaaaggc cccgacctgg tggctcagga
     1621 acacgacccc gaagagcgtt ccgaggttgc ccgtgccgaa catgacggcg accagcccag
     1681 aggtgagcgg cacggtcgag agccacagaa tccccatcgc cgccgcgaag acgagcacca
     1741 ccgtcggggt tgggggcagg gtgataaaga ccgcaataac ggcggccctt gcgaggtaga
     1801 tcccggccag caactgcctc ttgctgtggc gagcaccgag caccccggag ccgtaggatc
     1861 ccacgatgtt gaagagcccg atgagcgcga gggcccaggc cgcgatgcca ggcccggcca
     1921 tgtgcatccc gtcgtgcgag tgtccggcga cggcggcgac gtgcgccggc aggaacgtgg
     1981 tgatgaaggc cacgtgaaac ccgcacacga agaacccggc gacgagcagc aggtagctgc
     2041 cgtgcccgaa ggcttccctg acagcgtccc tggcgggcag agcccgcaca cccgttccct
     2101 cctgacggcc accacccctg agcgcggcgg cgagaaacgg aaccacgacc atggaccccg
     2161 ccagcagcaa gagcgtggtc tgccagccgt agtcggtaag gaaagcctgg cccagcggcg
     2221 cgaacacgaa ctgcccgagc gagccggcgg cggtcgcgag gcccatggcc tgcgagctcc
     2281 tctccgcggg aacgagcctc cccagggcgg cgatgacgat ggtgaaggag gcgccggaga
     2341 gcccgagccc gacgagcacg ccgcccgtga ggttgaaggc gagcggggtg gggctcaacg
     2401 ccatgagcgc cacaccaaca gcatagacga cgccgccagc ggcgagcacc cgcccagacc
     2461 caaagcggtc cgccacgccc ccggcaaaag gctgccctat accccacacg aggttctgga
     2521 tcgcaatggc cagcgcgaag acctccggcg accatcccct cccttcgccg atgggggtgg
     2581 cgaacagccc gaaactggtc ctcaaaccgt aggtgatcag ggcgatagta cacccacacc
     2641 ccacgacgaa ggccgtggac ctccaccacc tcacccgccc aacctccaaa cccactccct
     2701 ccccgaacca acgtattgtc caaaccctaa cgtatctgga acgatcggtc aaattagcct
     2761 gtagcgcgtt ccagcctgtc ggtcggccac cggcgccgct ttctccaagc tgatagctca
     2821 tcgcgaccgg cggcatacgt tagactcagg gagggagtcc gatctcaggg gggttatctt
     2881 ggcggagacg tgggaggtag tggtccaggg cgaggtgccg gccacgatgc gcgacggcac
     2941 ggtcctgatg tccaacgtgt acaggccggc gggtggtggg ccctatccgg tgctcctctc
     3001 caggcaccct tacggcaagg acatctcgtc cggcacctcg atgctcgacc cgataaaggc
     3061 ggcgaaggcc gggtacatcg tcgtggttca ggatgtgcgc ggtcgctacc gttctggggg
     3121 cagtttcatc tccttcgtca gcgagtatga ggacggctac gacagcgtcg agtgggcggc
     3181 ctcactcccc ggctccgacg gatccgtcgg catgcacggc ctctcgtact
//
LOCUS       DBNGVD010000611         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1267614, whole genome shotgun sequence.
ACCESSION   DBNGVD010000611 DBNGVD010000000
VERSION     DBNGVD010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1267614"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..586
                     /locus_tag="AC16CM_16280"
     CDS             335..586
                     /locus_tag="AC16CM_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141441.1"
                     /translation="MAYFEGQLKRAETEPGEEHTGYVLETEIDVSAIQDVEELVGDQI
                     AVTGDVEIVDFPERGKILVFRATSVAVLEDEEEEEVTEP"
     gene            complement(583..1380)
                     /locus_tag="AC16CM_16285"
     CDS             complement(583..1380)
                     /locus_tag="AC16CM_16285"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3141442.1"
                     /translation="MSGPSREQIEGLRSPLARVIEVHDAVSSTQERGRELARAGSPHG
                     TLVLAGVQEGGRGRLGRRWGSPRGGLWMSLVLRPGLEAGLASRITQTAAVGVAGALWE
                     IGVEARIKWPNDLLVDGRKICGILAESGVGPGGRLDYAVLGVGMNANLDPEDLGVTDR
                     GVTTIRYELCRDVSLLSLLDGILSNLDAALGRIQDFEAILDGWRALNCTLGNTVRVRR
                     SGETIEGRAVDLTGEGALLVATPGGTVELFEGEIEQIVREPSPGNGA"
     gene            complement(1377..2438)
                     /gene="cobT"
                     /locus_tag="AC16CM_16290"
     CDS             complement(1377..2438)
                     /gene="cobT"
                     /locus_tag="AC16CM_16290"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR3141443.1"
                     /translation="MAFEDRVQELAAEIPPPDEAAIRSARERHLMLSKPSGSLGRLEE
                     LGARLAGMAGECPPPVPESPAVLVCAGDHGVLWRGVSPWSQAVTAAMVGNFCDGGAAV
                     NAIAKTVGARVSVLDVGVASELGGHPLLRSAKVRRGTGDLSRGPAMSRDEAARAVMAG
                     AGVAEELIESGGVDLLVTGDMGIGNTTPAACLISSFTGNSPEQTTGRGTGIDDQTFEL
                     KVRVVTEALGMHDPDPGDPLGVLAAVGGLEHAALVGVVLMGAVYGVPVILDGVVSDSA
                     ALVAGALAPSSVGYLVAGHRSAEPGARISLGHLQLEPLLDLDMRLGEGTGGLLAVPLV
                     QAAARTLAEMATLEELGFG"
     gene            complement(2441..3364)
                     /locus_tag="AC16CM_16295"
     CDS             complement(2441..3364)
                     /locus_tag="AC16CM_16295"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIR3141444.1"
                     /translation="MDHTVGIAEENGSHKPEVGPALLVVGHGSRDTRGAREFHDLIGL
                     VRRRNPSLAVEGGFIELSRPPISECVDRLAEEGTRHVAAVPLMLLAAGHAKDDIPATL
                     VREKINHPEMTFRYGRALGIRPELLELMDERISAVVPEREKAETTVLVVGRGSSDPDA
                     NSDLAKVARLFYEGRPYPAVESAYISMTPPDVAAALERCRKLGAKRVVVFSYFLFTGV
                     LEERIREQAEAFARDNSGVEVRYAGYFGPDRRVADLLVERYTEALGGDIRMNCDVCVH
                     RVALPGFEEKVGAAATPHYHPDEPGHHSHHH"
     gene            complement(3366..>3599)
                     /locus_tag="AC16CM_16300"
     CDS             complement(3366..>3599)
                     /locus_tag="AC16CM_16300"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018736562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR3141445.1"
                     /translation="GDAIWVVGNAPTALFGLLDLEIAPALVVGLPVGFVGAAESKDAL
                     AASGLPAVTNRGPKGGSAVAAAALNALIYYEEE"
BASE COUNT          619 a         1262 c         1142 g          576 t
ORIGIN      
        1 cgatggcgaa gatgccgacg agcgtggcca gcactgcccc gatgccaacg tacatcaacg
       61 tcgcgctcat tcctctcgct tcctcctggc gtcgcccaat acaggtgtga ggggcctgtt
      121 tgaaagcgta ttaaatacca ggtcagaaaa gatagcaagc gagagtactc gcgtgtgccc
      181 cggacgatag tccgggcctc gacagaccat gatctcgttc ggatcctctc ccgaccaggt
      241 ggcgttccgg cagaggctgg cactgtgact tccatcccgc gaccgcgaat acgggtacta
      301 tatccccaca tgaaatcgag agaggggatc ggccatggcc tacttcgaag ggcagctcaa
      361 gcgcgcggaa acggagccgg gagaggaaca caccgggtac gttctggaga ccgagataga
      421 cgtatcggcc atacaggacg tcgaagagct ggtgggggac cagatcgcgg ttacggggga
      481 cgtggagatc gtggactttc ccgagcgggg caagatcctt gttttcaggg cgacttcggt
      541 ggccgtcctt gaggacgaag aggaagaaga ggttacggaa ccctaggctc cgtttcccgg
      601 actcggctct cgcacgatct gttcgatctc gccctcgaac aactctacgg tcccaccagg
      661 ggtggcgacc aggagcgcgc cctccccggt caggtccacg gcccggccct ctatcgtctc
      721 gcccgagcgc ctgacacgaa ccgtgttgcc aagggtgcag ttcagggccc gccacccgtc
      781 gaggatggct tcgaagtcct ggatgcgccc gagcgcggcg tcgaggttcg agaggatgcc
      841 atctagcagg gacagcaagc tcacgtcgcg gcagagttcg tagcgtatgg tcgtgacccc
      901 gcggtccgtc acccccagat cctccgggtc gaggttggcg ttcatgccca caccgagtac
      961 cgcgtagtcg aggcgtccac ccggccctac accagactcg gccaggatgc cgcagatctt
     1021 ccgcccgtcg acgagcaggt cgttgggcca tttgatcctg gcctctaccc cgatctccca
     1081 tagcgcccca gccaccccga cggcagcggt ctgggtgatg cgggaagcga gaccagcctc
     1141 gagcccgggc cgcaagacga gggacatcca gagtcctccc ctgggggaac cccagcgacg
     1201 tccgagcctt cccctacccc cctcctggac cccggcgagg actagcgttc cgtgggggga
     1261 tccggcacgg gcgagctcac ggccccgctc ctgggtggag gacacagcat cgtgcacctc
     1321 gatcacgcgg gcgagcgggc tgcggaggcc ctcgatctgt tccctggagg gaccgctcac
     1381 ccgaaaccca actcctccaa cgtcgccatc tcagccagcg tacgcgccgc cgcctgcacg
     1441 agcggcacgg cgagcaaacc tcccgtcccc tcacccagac gcatgtcgag gtcgaggagt
     1501 ggctcgagtt gcaggtgccc gagggagatc ctggcccccg gctctgccga gcgatgcccc
     1561 gccacgaggt agcccaccga gctcggggct agagcgccgg ccaccagcgc cgcagagtcg
     1621 gacacgacgc cgtcgaggat taccggcacg ccgtagacgg cgcccatgag taccaccccg
     1681 accagggccg cgtgctctag cccccccacc gcggccagaa ccccgagtgg gtctccgggg
     1741 tcggggtcgt gcatccctaa cgcctcggtc accaccctga ccttgagctc gaaggtctgg
     1801 tcatctattc cggtaccgcg gcctgtcgtt tgctccggcg aatttccggt gaagctggat
     1861 atgaggcagg ccgcaggcgt ggtgttgcct atgcccatgt cgcccgtcac caggaggtcc
     1921 acaccaccgc tctcgatcag ctcttccgcc accccggcac cggccatgac cgcccgcgca
     1981 gcctcgtccc gactcatggc ggggccgcgg ctcaggtcac ccgttccgcg ccgcactttc
     2041 gcgctcctca ggagcgggtg tccccccagc tcgctggcga cccccacgtc gagcacgctc
     2101 accctggccc ccaccgtctt cgcgatggcg tttaccgcag cccccccatc acagaaatta
     2161 cccaccatcg ccgccgtaac cgcctgggac cagggtgaga caccgcgcca gagcacgccg
     2221 tggtcgccgg cacagaccag gacggcgggg ctctccggca ccggcggcgg gcattcaccg
     2281 gccatccccg caagccgggc gcccagttcc tccaaccgtc ccaggcttcc ggatggcttg
     2341 gagagcatga ggtgacgctc cctggcgctc cggatggcag cctcgtcggg gggaggtatc
     2401 tccgccgcga gttcctgtac ccgatcctcg aaggccacgg ctagtggtgg tggctgtggt
     2461 gccccggctc gtcggggtgg tagtggggcg tcgcggcggc gcccaccttc tcctcgaagc
     2521 caggtagggc gacacggtgc acgcacacat cgcagttcat gcggatgtct ccccccaggg
     2581 cttcggtgta acgttcgacc agaaggtcag ccacacgccg gtccggcccg aagtacccgg
     2641 cgtagcgtac ctcgaccccg ctgttgtccc gggcgaaagc ctccgcctgc tcgcgaatac
     2701 gctcctccag gacgccggta aagaggaagt aggagaagac cacgacacgc tttgcgccga
     2761 gcttgcggca gcgttccagg gcggcggcga catccggcgg cgtcatgctg atgtatgcgc
     2821 tctcgaccgc gggatacggc cggccctcgt agaacaggcg ggccaccttg gcgaggtccg
     2881 agttggcgtc tgggtcggag gagccgcggc cgacaacgag aacggtggtc tctgccttct
     2941 ccctttcggg gactacggcg gagatacgct cgtccatcag ttcaaggagc tcggggcgga
     3001 tgccaagagc acggccgtac ctgaaggtca tctccgggtg gttgatcttc tcgcgcacca
     3061 gggtggcggg gatgtcgtcc ttggcgtggc ccgcggccag gagcatgagc ggaaccgcgg
     3121 cgacgtgccg cgttccctcc tccgcaagac ggtccacgca ctccgagatc gggggtctgg
     3181 agagctcgat aaagcctccc tcgaccgcaa gggacgggtt tcttcggcgc acgagcccga
     3241 tcaggtcgtg aaactctcgc gcgccgcgtg tatcccggct cccgtggccg acgaccagca
     3301 gcgccgggcc gacctcgggc ttgtgggatc cgttctcttc cgcaattccg accgtgtggt
     3361 ccactctatt cctcctcgta gtagatgagc gcgttcaggg ccgccgcggc aaccgccgag
     3421 ccgcctttcg gcccccgatt cgtgacggcc ggcaggccgc tcgccgccaa cgcatccttc
     3481 gactcggccg ccccgacaaa accgacagga agtccgacaa ctagcgcggg agcgatctcc
     3541 agatcgagca acccgaagag ggcggtcggc gcgttaccca ccacccagat agcatcacc
//
LOCUS       DBNGVD010000612         2453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_358713, whole genome shotgun sequence.
ACCESSION   DBNGVD010000612 DBNGVD010000000
VERSION     DBNGVD010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_358713"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="AC16CM_16305"
     CDS             complement(<1..1543)
                     /locus_tag="AC16CM_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HIR3141446.1"
                     /translation="MSEKVYRSELTPVSFLRRSAYMFPEKTSVVYGDRRYSYSEMEER
                     VDRLASRLRESGLEKGDRVAFLCPNTPPMLEGSFAVPAAGLVLVAINTHLGRDEVTYI
                     VEHSGARMVFVDAELEDLLDDVDSEVERVRIDDTGEPGDPYEDYLAEGSTGAVESVLD
                     DEEETISINYTSGTTGKPKGVMYTYRGAYLSALGNAIEVAMGYETRYLWTLPMFHCNG
                     WTFPWAVTAVAGTHVCLRKVEPPRIWELFEDENITHYCAAPTVQIGIANEDAAHPLER
                     SITAAIAGAPPSPTLLGGLLELNIRPMHIYGLTETYGPMTTSGVHPEWEDLDLDERAR
                     LMARQGQGYCTADLVRVVDENMQDVERDGETMGEIVMHGNMVAKGYFENEEATEEAFE
                     GGWYHSGDVAVWHPDGYIEIRDRNKDIIISGGENISTIEVEQVVARHPAVMEAAVVAM
                     PDEKWGERPKAFVVLKKGQEATEAEIIDFCKDHIARFKAPAAIEFGDLPKTSTGKVQK
                     FVLRDK"
     gene            1676..>2453
                     /locus_tag="AC16CM_16310"
     CDS             1676..>2453
                     /locus_tag="AC16CM_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR3141447.1"
                     /translation="MDTPERHRLERHARRIEELRLWRSAYESHVKAWRFVAGEGKAHD
                     LQPGDFWPEIGIPVRLSARTRVPEEWAGLPVELELWLGGEGFVEISVGDGRTSGGLNP
                     FHRAFPVLDEARGGEPVEISAEVVSKGLFGSNVSEPRLELARLVVPQREVRALERDLT
                     AVFETCAALDDHEAVPRLLDALDEVAAVLATAWPTGTDVTLTRYLEGFAATAGQKAGS
                     LPPTYAEKLVEINRHLGEPWSLPPAPEALAPLPERAGEAVR"
BASE COUNT          373 a          811 c          799 g          470 t
ORIGIN      
        1 ctttgtcgcg cagcacgaac ttctggacct tgccggtcga ggtcttgggc aggtcgccga
       61 actcgatggc ggccggggct ttgaagcggg cgatgtggtc cttgcagaag tctattatct
      121 ccgcttcggt cgcctcctgc cctttcttga ggacaacgaa cgccttgggg cgctcgcccc
      181 acttctcgtc gggcatcgcg acgaccgcag cctccatgac ggccggatgc cgtgcgacga
      241 cctgctccac ctcgatggtg gagatgttct cgccgcccga gatgatgatg tccttgttcc
      301 tgtcccggat ctcgatgtat ccatccgggt gccagactgc cacgtctccg gagtggtacc
      361 agccgccctc gaacgcctcc tcggttgcct cctcgttctc gaagtaaccc ttggcgacca
      421 tatttccgtg catgacgatc tcacccatcg tctcgccgtc gcgctcgacg tcctgcatgt
      481 tctcgtcaac cacgcgcacg aggtcggcgg tgcaataacc ctgaccctgg cgcgccatga
      541 gccgtgcccg ctcgtccagg tccaggtcct cccactcggg atggacgccg ctcgtggtca
      601 tcgggccgta ggtctcggtc aggccgtaga tgtgcatggg tcggatgttc agctctagca
      661 gccccccgag aagggtgggg gagggcgggg cgcccgctat ggcggcggtg atcgatctct
      721 ccagtgggtg tgccgcgtcc tcgttagcga tgccgatctg aaccgtgggc gcggcgcagt
      781 agtgcgtgat gttctcgtcc tcgaagagtt cccagatgcg tggcggctcg accttcctga
      841 ggcacacgtg cgtccccgcc acagcggtta cggcccaggg gaaggtccac ccgttgcagt
      901 ggaacatggg cagcgtccac aggtacctgg tctcgtaacc catcgcgacc tcgatggcgt
      961 tgccgagggc gctcaggtag gcaccacggt acgtgtacat gacgcccttc ggctttcctg
     1021 tcgttccaga ggtgtagttg atcgagatgg tctcttcctc gtcatcgagc acgctctcta
     1081 cggcaccggt agatccctcc gccaggtagt cctcgtaggg gtcgccaggc tctccggtgt
     1141 cgtcgatgcg aacccgctcg acctcgctat ccacgtcgtc cagcaggtct tccagctccg
     1201 cgtccacgaa gaccatcctg gccccggagt gctcgactat gtaggtgacc tcgtcccggc
     1261 caaggtgggt gtttatggcg acgagcacga ggcccgcggc cggcacggcg aagctgcctt
     1321 cgagcatggg tggtgtgttg gggcacagga acgccaccct atcgcccttc tcgagccccg
     1381 actcccgcag gcgggaggcc aggcggtcga cgcgctcctc catctcactg taggagtagc
     1441 ggcgatcgcc gtagaccacc gaggtcttct cggggaacat gtaggcgctg cgcctgagga
     1501 agctgaccgg cgtaagctcg ctgcggtaga ccttctcgct caaaagacac tccttttttc
     1561 tctctccctc ccgcagtata gcgttcacga cgccttgttt gtcgccgcgt ccgaagcccg
     1621 aaggcgccag agcggctaat atctggggct gtcgcctgcg agtagaagga gagagatgga
     1681 tacccccgag agacatcgcc tggagcgtca tgcccgccgc atcgaggagt tgaggctctg
     1741 gcgcagcgcc tacgagagcc acgtgaaggc gtggcggttc gtcgccggtg agggcaaggc
     1801 gcacgacctc cagccgggag atttctggcc ggagataggt atcccggttc gtctctcggc
     1861 ccgcacgagg gttcccgaag aatgggccgg tctccccgtg gagctggagc tctggctcgg
     1921 cggcgagggt ttcgtggaga tctccgtggg cgacggacgg acttcggggg gcctgaaccc
     1981 cttccatcgg gccttccccg tgctcgacga ggcgcggggc ggtgagcccg tcgagatctc
     2041 tgccgaggtc gtctccaagg gccttttcgg gtccaacgtc tcggaaccac gcctggagct
     2101 ggcgcggctg gtcgtcccgc aaagggaggt gagggccctg gagcgggacc ttacggccgt
     2161 attcgagacc tgcgccgccc tcgacgacca cgaggcggtg ccgcgcctgc tcgacgcgct
     2221 cgacgaggtc gctgcggtcc ttgccacggc ctggccgacg ggcacggacg tgaccctgac
     2281 ccgttacctc gaaggcttcg ccgcgaccgc cgggcaaaaa gcgggcagcc tcccgccgac
     2341 ctacgccgag aagctagtgg agatcaaccg tcatctcggt gagccctgga gccttccgcc
     2401 ggcgcctgag gcgctggccc ctcttcccga gagagccggg gaggccgtcc gcg
//
LOCUS       DBNGVD010000613         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2180976, whole genome shotgun sequence.
ACCESSION   DBNGVD010000613 DBNGVD010000000
VERSION     DBNGVD010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2180976"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..>2432
                     /locus_tag="AC16CM_16315"
     CDS             220..>2432
                     /locus_tag="AC16CM_16315"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIR3141448.1"
                     /translation="MPERFPEVRCANPVTLEDPAAFLDEELPAARVVLVRLLGGRRAW
                     PDGLEELGRRCARLGVPLLAFGGEAEPDAELTALSTVPSGTVLEAFEYLRHGGVGNTE
                     NLLRFVADTVLMEGYGFEPASPLPEVGVYHPRVPDGSPVEELIAHHDPARPTVGVIFY
                     RAHWMSGNTDFVDDLASALERAGANALPVYCYSLRSGADGTVPAVELLKDRVDCLVTT
                     VLAGGGSNAADAHRSGSPEEWLEWEVPALEELGVPVLQGISTTSTREAWLASDAGLTP
                     LDTAWQVAIPEFDGRIIGVPFSFKERLGEGSPVGAPLTLYRADTERTSRLAGLAARFA
                     GLGRRANVEKKVAILLSNYPTKHSRVGNAVGLDTPASAIGLLDAMREAGYIVDGAPDD
                     GDELIHSLIAAGGHDLEFLTGDQLGNAAGRLDTGRYAEWFARLPEGLRSDIEEHWGPP
                     PGDLYVDDGDFVLAGLTFGNVFVGIQPPRGFGENPIAIYHDPDLPPTHHYLAAYWWMI
                     EEFGADAVVHLGKHGTLEWLPGKSLGLSSTCAPDAVLRDVPLFYPFVVNDPGEGTQAK
                     RRAHATVVDHLIPPMTRADTYDDLAKLEQLLDEYYQVETLDPSKLPAIRVSIWETLRD
                     AELHRDLGVEEQPEEFSDFLTHVDGYLCEIKDLPIRGGLHVLGEAPEGEPLHHLIASI
                     LRLGAGEIPGLRRAIANAFDLDERFMVENGGAKVEAPAALAERFPGTVATASDLL"
BASE COUNT          389 a          846 c          794 g          403 t
ORIGIN      
        1 catcagcttt tgcctctcgg ttcgtcgccc ggcgtcgcaa acgcgagcgg ggaggttgcc
       61 gaccgttcgt tttcccgatg cgacgcttct gcctctaccg taccgtcgcc ggttacttgc
      121 tgagggctga aagctgaaag cgcagcagga gccgacaggc tcctgctgct aaccaccgcc
      181 gacaccgaga tcctggccgc gtcaaaggcc tcggagcgta tgcccgaaag attccccgag
      241 gtcaggtgcg ccaacccggt tacgcttgag gacccggcgg ctttcctcga cgaagagctg
      301 cccgcggcga gggtggtgct tgtgcgcctt cttggcggga ggagggcgtg gcccgatggt
      361 ctggaggagc tggggcggcg ttgcgcgcgg cttggcgtcc cgctgctcgc cttcggcggg
      421 gaggccgagc cagacgcgga gcttacggcg ctctccaccg tgccatcggg taccgtgctc
      481 gaggcgttcg agtacctgcg ccatggcggg gttggcaaca cggagaacct gctccgcttc
      541 gtcgcagaca ccgttctcat ggagggctac gggttcgagc cggcctcgcc tctgccggag
      601 gtcggggtct accacccccg ggttccggac ggctctccgg tcgaggagtt gatcgcgcat
      661 cacgaccccg cccggcccac ggtgggcgtg atcttctacc gcgcgcactg gatgagcggg
      721 aatacagact tcgtagatga tctcgcctcg gcgctggaga gggcaggcgc caacgccctg
      781 ccggtctact gctactcgct gcgctccggc gcggacggca cggtaccggc cgtcgagctt
      841 ctgaaagatc gggtagattg cctggtaacc accgtcctcg cgggtggggg atccaacgcc
      901 gccgacgccc accgttcggg ctcgccggag gagtggctgg agtgggaggt ccccgccctg
      961 gaagagctcg gggttcccgt gttgcagggt atctccacga cctcaacacg tgaggcctgg
     1021 ctcgcctccg acgccggcct cacgcccctt gacacggcct ggcaggtggc gatccccgag
     1081 ttcgatgggc gcatcatcgg ggtccctttc tccttcaaag aacgactcgg cgaaggctcg
     1141 cccgtcggcg caccgctcac cctctaccgg gccgatacag agcgtacgtc gcgcctcgcc
     1201 gggctcgcgg cgcgattcgc cggtctcgga cgccgcgcca acgtggagaa gaaggtcgcc
     1261 atcctgctct ccaactaccc gaccaagcac tcgcgggtgg gcaacgccgt cgggctggat
     1321 acccccgcga gcgccatcgg cctgctcgac gccatgcgag aggctggcta catcgtagac
     1381 ggcgcacccg acgacggcga cgagctcatc cattcgctaa ttgccgccgg gggacacgac
     1441 ctggagttcc tgacgggcga ccagctcgga aacgccgccg gaaggcttga cacgggacgc
     1501 tacgctgagt ggttcgcccg gctgccggaa ggcttgagaa gcgacatcga ggagcactgg
     1561 ggacccccgc ctggggacct ttacgtggac gacggcgact tcgtgttggc cgggctcacc
     1621 ttcgggaacg ttttcgtcgg cattcagccg ccacgggggt tcggtgagaa ccccatagcc
     1681 atctaccacg atccggacct cccgcccacc caccactacc tggcggccta ctggtggatg
     1741 atcgaggagt tcggggcaga cgccgtggtc cacctcggca agcacggcac cctggagtgg
     1801 ttgcccggca agtcccttgg cctctcgtcc acctgcgcac ccgacgcggt cctgcgcgac
     1861 gtgccgctct tctacccgtt cgtcgtcaac gatcccggcg aggggaccca ggccaagcgt
     1921 agggcgcacg ccacggtggt ggaccacctg atccccccga tgacccgcgc cgatacctac
     1981 gacgacctgg cgaagctgga gcaactcctc gacgagtact accaggttga gacgctcgac
     2041 ccctcgaagc taccagcgat aagggtcagt atctgggaga ccctccgcga cgcggagctg
     2101 caccgggacc tcggcgtcga agaacagccc gaagagttct cggatttcct cacccacgtg
     2161 gacgggtatc tctgcgagat caaagacctc cccatccgcg gcggtctcca cgtcctcggc
     2221 gaagcccccg agggcgagcc cctgcaccac ctgatcgcct ccatcctgcg cctcggcgcc
     2281 ggcgaaatcc ccggcctgcg ccgcgccatt gccaacgcgt tcgatctcga cgagaggttt
     2341 atggtcgaga acggtggtgc gaaggtcgaa gccccggcgg cgctcgccga acgcttcccc
     2401 ggcacggtgg ccacggcttc ggacctgctc ga
//
LOCUS       DBNGVD010000614         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_176843, whole genome shotgun sequence.
ACCESSION   DBNGVD010000614 DBNGVD010000000
VERSION     DBNGVD010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_176843"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="AC16CM_16320"
     CDS             <1..1085
                     /locus_tag="AC16CM_16320"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3141449.1"
                     /translation="RVLAYDPYVSEDRMRSMNVGRADTADEVFERADFVSLHVPRTPQ
                     TVGLVDENALSKMRPTAYLINVARGGIVDETALYNALKEGTIAGAALDVFAEEPTTES
                     PIFNLPNVVVTPHLGASTAEAQDRAGTTAAEQVATALGGSVPIHAINAPVPVGEGAEF
                     VAQFSGLCEALGSLLYQLSDKASDTLRIDYRGEVAGFDTRLLDVSAQKGLLASMVFEP
                     LNFVNTPALARERGLKVETSRSSESADYTNLVELRMGENTVSGTLFGPRMQPRLVGAM
                     GFDFDVVPEKYMLFIRNEDRPGMIGKLGGILGEHGINIGNMAVGRGEPGSRAAMAVTV
                     DEPVPAEVLKALERTPGFDDARSVTL"
     gene            1085..1297
                     /locus_tag="AC16CM_16325"
     CDS             1085..1297
                     /locus_tag="AC16CM_16325"
                     /inference="COORDINATES: protein motif:HMM:NF025533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HIR3141450.1"
                     /translation="MARLDDIIARYAPFGPTFGPGDKAPFSGTYLCDRGTMLPPARVQ
                     LSKGEEFPEAEGLGEHTRWRQTNIQA"
     gene            1407..2036
                     /locus_tag="AC16CM_16330"
     CDS             1407..2036
                     /locus_tag="AC16CM_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR3141451.1"
                     /translation="MGKRGGGMGTTLVLALVIVVLGVALGVGLARFGGVLPLVGPLLG
                     ERPPRTTTGPVVVEGIRELDQLATVRWTESVPVTRESGGDLMERLFDGERVLVIATGN
                     VEAGIDIGDVGRDDVKVDGDTVTIDLPEAEILSASLDEEKTSLYDRDLSPLNLRPDDD
                     LVEEARVRAVEKISASARQNGILDAAEGNAEDSIRAFLITLGFERVEFR"
BASE COUNT          401 a          707 c          733 g          379 t
ORIGIN      
        1 tgcgcgttct ggcctacgac ccctacgtct ccgaggacag gatgcgctcc atgaacgtcg
       61 ggcgcgctga cactgcggac gaggtcttcg agcgggccga cttcgtctcg ttgcatgtgc
      121 cgcgtacccc gcaaaccgtg gggcttgtcg acgagaacgc gctttcgaag atgaggccga
      181 cggcctacct cataaacgtg gcgcgcggcg gcatcgttga cgagacggcg ctctacaatg
      241 ccctcaaaga aggcaccatc gcgggcgcgg cgctcgacgt gttcgccgag gagccgacga
      301 cggagtcacc gatcttcaac ctgcccaacg tcgtcgtaac gccccacctc ggggccagca
      361 ccgccgaggc gcaggacagg gccggcacga cggccgcgga gcaggtcgcc accgcccttg
      421 gcggctccgt cccaatccac gccataaacg cccccgttcc ggtgggtgag ggggccgagt
      481 tcgtggccca gttctcgggg ctctgcgagg cgcttggcag cctgctctac cagctctccg
      541 acaaggcctc cgacacgctc aggatcgatt accgcggcga ggtcgccggc ttcgacacgc
      601 gcctcctcga cgtctcggcc cagaagggcc tcctcgcgtc catggtcttt gagccactca
      661 acttcgtaaa cacgccggcg ctggcaaggg agaggggcct caaggtggag acctcgcgct
      721 cctcggagtc agccgactac accaacctcg tggagctacg catgggagag aacaccgtca
      781 gcggcaccct cttcggaccc aggatgcagc cacggctcgt gggggccatg ggcttcgact
      841 tcgacgtggt acccgagaag tacatgctct ttatccgcaa cgaggaccgc ccgggtatga
      901 tcggcaagct cggtggcatc ctcggggagc acggcatcaa catcggcaat atggccgtcg
      961 ggcgtgggga gcccggctcc cgcgcggcga tggccgttac cgtagacgaa cccgtccccg
     1021 cggaagtgct gaaagccctg gagagaaccc cgggcttcga cgatgcccgc tcggtgaccc
     1081 tgtgatggcc cgccttgacg acataatcgc ccgctacgca cccttcggtc ccaccttcgg
     1141 tccgggcgat aaagcaccct tctcgggcac ctacctctgc gacaggggta ccatgctgcc
     1201 ccccgccagg gtccagcttt ccaaaggcga agagttcccc gaagcagaag gactgggcga
     1261 acacacccgc tggcgccaga ctaatattca agcctagagc ggttcgcttc gcgaacctcg
     1321 ccgtcagccg ttagctttca gctcttcgct gacggcctat agctcgatgt agaatctccg
     1381 tatctgtacc ctcggaaagg ctgggcatgg gcaagagagg cggcgggatg gggaccacgc
     1441 ttgttctggc gctcgtcatc gtagttctcg gcgtcgcgct aggcgtcggg ctcgcgcgtt
     1501 tcgggggggt gttgccgctg gtcgggccgc tgctggggga gaggccgccg cggaccacga
     1561 cagggcctgt ggtggtggag ggaataaggg aactcgacca gctggcgacc gtgcgttgga
     1621 ccgagtccgt gccggtaacc agggagtcgg gcggggacct tatggagagg ctgttcgacg
     1681 gcgagagggt gctcgtgatc gccacgggca acgtcgaagc tggcatcgac attggcgacg
     1741 tggggcggga tgacgtgaag gtcgatggcg ataccgtaac catcgatctg ccagaagccg
     1801 agatcctctc cgccagcctc gatgaggaga agaccagcct ctacgaccgg gacctcagcc
     1861 ccctcaacct ccgtccggac gacgacctcg tggaggaggc ccgcgttcgg gcggtggaga
     1921 agatctcggc ctccgcccgc cagaacggga tcctggacgc cgcagaagga aacgcggaag
     1981 acagcatccg cgccttcctc atcacgcttg gtttcgagag ggtagaattc cgttagggtg
     2041 gagtagggag accgtctggc gctgatccag agacagcgtc tttgcgtatc ccgagcgaag
     2101 gaggttgttt atgatcgaag aggaccggga tacgcgcgag cgggtcagcc ccgagacgag
     2161 ccggggccat tacttgctgg agatgttcgg catattctgc tttgcggctc tctcgttgct
//
LOCUS       DBNGVD010000615         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4733329, whole genome shotgun sequence.
ACCESSION   DBNGVD010000615 DBNGVD010000000
VERSION     DBNGVD010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4733329"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16CM_16335"
     CDS             <1..394
                     /locus_tag="AC16CM_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3141452.1"
                     /translation="ASVPDEIVLADTIGVGVPSQVRNLVEGVARHGLMVGCHFHDTRN
                     TGIANAVAAVESGARVLDASVGGTGGCPFAPRATGNIATEDLVYLLHNMGYETGIDLD
                     ALIDVASWLERQLDKELPGQLYKAGNFG"
     gene            554..2605
                     /locus_tag="AC16CM_16340"
     CDS             554..2605
                     /locus_tag="AC16CM_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3141453.1"
                     /translation="MYNVAVDVGGTFTDVLVFDEETGGLTEGKVLSTPDDPSKGVVEG
                     IEAVCEKVGISFPDLHLFFHGTTVVTNMILTNTGSRVGLLTTKGHEQILHLARAWTPG
                     PLYGWMALEKPDPPADPTDTVGISERITASGEVLEELDEDEVRGAVNRLVERGVESLA
                     IGFLNSYVNPAHERRARDIVREAHPDLSVSISADIGQEYGEYERTLTTVVNTAVQPGT
                     SRYMRNFEGSIGEKRFGGALSIVRSDGGAMSTEAVADSPIQIALSGPSGGVTGSAYLA
                     RRIDVPDILTFDMGGTSTDVSLCRDGETPVRRDIQLGYFQFKSPAVDVHSVGAGGGSI
                     AFLSASGALQVGPASAGADPGPAAYGRGGEKPTVTDANVVLHRIPPGVALGGTLQMDE
                     EAARVAVGTIAEQLGMGVEEAAEAILEIVNENMHAALRVVSVERGHDPRNFGLVAFGG
                     AGPMHANALGRLIRSRSVIVPPTPGVMSAFGFLTSDIQNEFPETYLRVAEETPASELK
                     ETVERLIAQADGWLEGEGVPETDKRFDLYADCRYYLQNIQIPCVFELEELREGNSDFL
                     RERFEEAHRQRYNFDLPDSPLEMATVRVVGRGTIKGVSLLESEEEAGEDPSSAVTRQD
                     RVYFEGGWLDTPIYDRARLEPGNVVAGPAVVVQDDTTTVLEPGYAGTVDRFGNILIEE
                     A"
     gene            2610..>4136
                     /locus_tag="AC16CM_16345"
     CDS             2610..>4136
                     /locus_tag="AC16CM_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006066974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3141454.1"
                     /translation="MVLQAEPDFVRDHDIDNVTLDIIENALKNIRHEMDRVLVTTAVS
                     PVIREQADEFPLIADRKGRMVVGQFGSPVDTVLENSPYTVEDLKDGDVIALNDPYMME
                     GSTSHLPDILLVRPIFYDDGGGPDRIGYALQWGNLMDVGGSTAGSIPIDARSIYEEGV
                     RMPPVKLYDAGRLNEEVLRFFCHNSRTPRETRADVMAIAAGTATGAQRVKDICDRFGK
                     DTYLEACDALLARTRASIINLIRQLIPEDERFEFEDYTDDDGLGNGPIKLRLAMWREG
                     DELHLDWTGTDAQVPGPVNFLLNKEMFQMFAGVFLISAVDPTILFNDGYADIIKVNIP
                     DGSVLRPKEPAPLSNRLVVMARQFDVLGAVYAKAIGFNVSGSYGTSPNFVYSGVKKDG
                     EPFQTMEILYGGIPAIPGKDGLDGHSWWPEFLAVPAEYMETYYPMIVEEYSVRRDSCG
                     AGTFRGGCGIKKVYRFLAGGRITYQDDRHHTYPYGVDGGKPGAASKKTLIRAGGERIE
                     M"
BASE COUNT          808 a         1332 c         1334 g          662 t
ORIGIN      
        1 ggcttccgtc ccggacgaga tagtactggc cgacaccata ggggtcggcg tgccaagcca
       61 ggtacgcaat ctcgtcgagg gcgtcgccag gcatggcctc atggtaggtt gccacttcca
      121 cgacacccgc aacaccggca tagccaacgc cgtcgccgcc gtcgagagcg gcgccagggt
      181 tctggacgcc tccgtaggcg gcaccggcgg ctgccccttc gccccacgcg ccaccgggaa
      241 catagccacg gaagacctcg tgtacctgct ccacaacatg ggctatgaga cgggaatcga
      301 cctggatgcc ctgatagatg tggcctcgtg gctagagcgc caactcgaca aagagctacc
      361 gggccagctc tacaaggcgg gaaatttcgg atgagctatc agctattagc aatcagcaat
      421 cagccagaga tcaaggatat cgcatctttg cggcaacgaa cgtttctcaa ccaacgctgg
      481 cttacgagca acggcttcgc gaactccaga gctgacggct aatagctgat tgcttccgac
      541 cgaagggaga acaatgtaca acgtggcggt agatgtcggc ggcaccttca ccgacgtgct
      601 cgtcttcgac gaggagactg gtgggctaac cgagggcaag gtgctctcga cgccggatga
      661 cccctcaaaa ggggtggtcg aagggatcga ggccgtgtgc gagaaggttg ggatctcgtt
      721 ccccgatctg cacctgttct tccacggcac gaccgtggtg acgaacatga tcctgaccaa
      781 cacgggctcg cgcgtcggcc tactgaccac gaaggggcac gagcagatcc tgcacctggc
      841 ccgcgcctgg acccccgggc cgctctacgg ctggatggcc ctcgagaagc cggacccacc
      901 cgccgacccg accgacaccg tgggtatctc cgagcgcatc acagcctcag gcgaagtact
      961 ggaagagctg gacgaagacg aggtgcgcgg ggctgtgaac aggctggtcg agcggggcgt
     1021 ggagtcgctc gccatcggtt ttctgaactc ctacgtcaac cccgcgcacg agcggagggc
     1081 cagggacatc gtgcgcgagg cccatcccga cctctccgtc tccatctccg ccgacatcgg
     1141 gcaggagtac ggtgagtacg agcggacgct caccacggtc gttaacaccg cggtgcagcc
     1201 tggcaccagc cgctacatga ggaacttcga gggttccata ggggagaaga ggttcggggg
     1261 ggcgctctcc atcgtgcgct ccgacggcgg ggccatgagc acggaggccg tggcggacag
     1321 cccgatccag atcgcgctct cggggccatc cggcggcgtt accggctccg cttatctggc
     1381 gcgccggata gatgtccccg acatactgac cttcgatatg gggggcacct cgacggacgt
     1441 atcgctgtgc cgcgacggcg agacgccagt caggcgagac atccagctcg gctacttcca
     1501 gttcaagtca ccggccgtcg acgtgcacag cgttggcgcc ggcggtggct ctatagcctt
     1561 cctttccgcg agcggtgccc tgcaggtggg gccggcgagc gccggtgctg acccgggacc
     1621 agccgcctac ggccgcggcg gtgagaagcc gacggtaacc gacgccaacg tcgtgttgca
     1681 ccgcatcccg ccaggcgtcg ccctcggcgg cacgctccag atggatgagg aggccgcgcg
     1741 ggtggccgtc gggaccatcg ccgagcaact aggaatgggc gtcgaggagg ccgccgaggc
     1801 catactggag atcgtcaacg agaacatgca cgctgcgctc cgggtcgtca gcgtcgagcg
     1861 cgggcacgat ccaaggaact tcgggctcgt cgccttcggc ggggccggtc ccatgcacgc
     1921 caacgccctc gggcggctca tccgttcacg ctctgtcatc gtgccgccaa cccccggggt
     1981 gatgagcgct ttcggcttcc tcacctccga catccagaac gagttcccgg agacctacct
     2041 gcgcgtcgcc gaggagaccc cggcctcaga gctcaaggag accgtggagc ggttgatcgc
     2101 ccaggccgac gggtggctcg aaggcgaggg cgtgccggag acggacaagc ggttcgacct
     2161 gtacgccgac tgccgatact acctgcagaa cattcagatc ccctgcgtgt tcgagctaga
     2221 agagctgcgg gaggggaaca gcgactttct gcgcgaacgt ttcgaagagg cgcacagaca
     2281 acgctacaac ttcgacctgc ccgactcgcc gctcgagatg gccaccgtcc gcgtcgtggg
     2341 gcgcggaacg atcaagggcg tgagcctgct tgagagcgag gaggaggcag gtgaggaccc
     2401 atcgagcgct gtaacgcgcc aggacagggt ctacttcgaa ggcgggtggc tggacacccc
     2461 gatctacgac cgggccaggc tagaacccgg aaacgtggtc gccggtcccg cggtcgtggt
     2521 gcaagacgac accacgacgg ttctggagcc gggttacgcc ggcacagtgg accgtttcgg
     2581 aaacatcctg atcgaggagg cgtagagaga tggtgctgca ggccgagccg gacttcgtca
     2641 gagaccacga catagacaac gtcaccctgg acattatcga gaacgccctg aagaatatcc
     2701 gccacgagat ggaccgggtg cttgtaacga ccgccgtcag ccccgttatc cgcgagcagg
     2761 ccgacgagtt cccccttata gccgaccgca agggccgcat ggtcgtcggt cagttcggga
     2821 gccccgtcga tacggtactg gagaactccc cttacaccgt cgaggacctg aaggacggcg
     2881 acgtcatagc gctgaacgac ccgtacatga tggaggggag cacgtcgcac ctccccgata
     2941 tcctgctcgt acgacccatc ttctacgatg acggcggggg gcctgaccgc atcggatacg
     3001 cgttgcaatg gggcaacctg atggatgtgg gcggttcgac ggccgggtcc atccccatag
     3061 acgcccgctc catctacgag gagggcgtca ggatgccgcc ggtcaagctc tacgacgcgg
     3121 gccggctcaa cgaggaggtc ctccggttct tctgccataa ctcccgcacc ccgcgcgaga
     3181 cccgcgccga cgtcatggcc atagcagccg ggaccgccac gggagcccag cgcgtcaagg
     3241 acatctgcga ccggttcggc aaggacacct acctggaggc ctgtgatgcg ctcctggcga
     3301 ggaccagggc gagcatcatc aaccttatcc gccagctcat acccgaggat gagcgcttcg
     3361 agttcgaaga ctatacagac gacgacggcc ttggcaacgg tcccatcaag ctaaggctcg
     3421 cgatgtggcg ggagggcgac gagctgcacc tcgactggac cggcacagac gcccaggtgc
     3481 caggccccgt caacttcctg ctcaacaaag agatgtttca gatgttcgcc ggggtgttcc
     3541 tgatctccgc cgtggacccc acgatcctgt tcaacgacgg ctacgccgac attatcaagg
     3601 tcaacatccc ggacggctcg gtcttgaggc cgaaagaacc ggcgcccctg agcaaccgcc
     3661 tcgtcgtgat ggcgcggcag ttcgacgtgc tcggcgccgt ctacgccaag gccattggct
     3721 tcaacgtctc aggctcctac gggaccagcc ccaactttgt ctactcgggc gtcaagaagg
     3781 acggagagcc gttccagacc atggagatcc tctacggtgg tatcccggcc atccccggca
     3841 aagacggcct ggatgggcac tcctggtggc ccgagttcct ggcggtgccg gcggagtaca
     3901 tggagaccta ctacccgatg atcgtggagg agtactcggt ccgtcgcgac tcctgcgggg
     3961 ccggcacttt caggggtggc tgcggtatca agaaggtcta ccgcttcctg gccggtggcc
     4021 gcatcaccta ccaggacgat cgccaccaca cctaccctta cggcgtggac ggcggcaagc
     4081 ctggcgcggc gtcgaagaag accctcataa gggccggtgg tgaacgtatc gagatg
//
LOCUS       DBNGVD010000616         5142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4550181, whole genome shotgun sequence.
ACCESSION   DBNGVD010000616 DBNGVD010000000
VERSION     DBNGVD010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4550181"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16CM_16350"
     CDS             <1..231
                     /locus_tag="AC16CM_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3141455.1"
                     /translation="ELGNRNQVLTITHLPQIASEAGTHVVVSKEESEGRTITRIGRIE
                     GEERRRELARMLSGRIDEASLAHATELLVSGR"
     gene            complement(294..602)
                     /locus_tag="AC16CM_16355"
     CDS             complement(294..602)
                     /locus_tag="AC16CM_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3141456.1"
                     /translation="MVVPPKVFRGPQKADGLTERELEVLLMVARDMSNDQIAGSLYTS
                     GATAKRHLANIFPKIGASSRAEAVRIALAEEWISPRDITGTDTNMAWSGPAIHTALWR
                     "
     gene            complement(711..1034)
                     /locus_tag="AC16CM_16360"
     CDS             complement(711..1034)
                     /locus_tag="AC16CM_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3141457.1"
                     /translation="MNEDATRDKPAIRVLVADDHAMFRQGVVEMLRTAEEIEVVGEAE
                     IGERAVAMIKRLVPDVVLLDVDMPLMGAEEVMRLMMNDPPEDASTPRVSIVTMHDEPG
                     LVREL"
     gene            1352..3001
                     /locus_tag="AC16CM_16365"
     CDS             1352..3001
                     /locus_tag="AC16CM_16365"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3141458.1"
                     /translation="MSETKYIFVTGGVVSSIGKGTSAAALGMLLKRRGYRVVLQKFDP
                     YINVDPGTMNPYQHGEVFVTEDGAETDLDLGHYERFLDENLGKLSNVTTGSVYWEVIA
                     RERRGDYLGATVQVIPHITNEIKNRIGRLGQDKDIVITEIGGTVGDIESLPFLEAIRQ
                     FRNDVGRKNVLYVHVSYVPYIEAAGELKTKPTQHSAQRLREIGISPDVLICRADRPIS
                     EDIRRKIALFADTELDSVIAAEDAPNLYAIPLSLHEEGLDDLVIEKLGLDAPNADLEE
                     WRRLVSRMLEARRTVKVAIIGKYIKLQDAYLSVVEALAHAGGSHDLKVELDWVDSEQL
                     VDRESTEQRLAGVDGVLVLPGFGHRGAEGKIEAARYARETGTPYLGLCLGMQIEVIEF
                     ARNAAGLEMANSTEFEQDTPHPVIDIMPDQVGVEMGGTMRLGHWPCQIVPGTLAEEVY
                     GTELVGERHRHRYEVNNAYRDALENAGMVFSGTSPDGRLVEIAEIADHLFFIGSQFHP
                     EFKSRPLRPHPLFSGFVGACKAASDEPVDDTVVVPEERAAR"
     gene            3055..3609
                     /locus_tag="AC16CM_16370"
     CDS             3055..3609
                     /locus_tag="AC16CM_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141459.1"
                     /translation="MRPTPDPSGTVAIRSAGLVEFRAKLGYRSADEVPRGPTLGAPPS
                     GARLSVVETDDGVTREILGEILALSEADLGRALEGVLRSLTIAAALTDAVLETSAARV
                     VPVRTAPAGPLQTLALDVLEAGFRVRFAPTWTPLSEGELGLGVGNDEGNLRAIFGAQL
                     PWSLVGTGAVSGGSTARYFGEEEL"
     gene            3606..4739
                     /gene="ald"
                     /locus_tag="AC16CM_16375"
     CDS             3606..4739
                     /gene="ald"
                     /locus_tag="AC16CM_16375"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3141460.1"
                     /translation="MRIGVPRERTPGESRVALAPHAVYELTRVGHRVFIEAGAGEASS
                     ISDEAYDQAGGRIVDDPARLYGDSEIVVKVYGPLEEEFAYLREGLVLVSFLSLPVNPG
                     LMDALLDSRCVAFAMETIRDERGGLPVLTPMSEIAGRLTPQIGAHYLQRPAGGRGILL
                     GGSTGVRPGRVVILGCGIVGSGAARVASGLGASVVVIDRDLDRLRRLEQFRLPNVTTL
                     ASSSLGIRDAVLRSDLLIGAVHVVGARTPALVARDTVSEMRDGSVIVDVDVDYGGCVE
                     TSRPTTLSDPTFVEYGVTHYCVKNVPAAVPVTATRALSNALLPLMRRLAGVGLPDALA
                     ADEGLARGVVVAEGRVVDAVLARATGTEHNRLSSVLPLNSEAR"
     gene            4736..>5142
                     /locus_tag="AC16CM_16380"
     CDS             4736..>5142
                     /locus_tag="AC16CM_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIR3141461.1"
                     /translation="MTFEPQGRSLADFTVELDVYSGPYEWLLALILKDELEIFEIPLR
                     ELVESYVGARERDPAGGGLERDTDFAGSASALVLLKSRTLSPALDPDAEGDEDETLSP
                     QELAERLTEYLKIRRAAEHLRARFAANAGHHPSS"
BASE COUNT          890 a         1694 c         1702 g          856 t
ORIGIN      
        1 gagcttggca accgcaacca ggtactgacc atcacccacc tgccgcagat agcatcggag
       61 gctggcaccc acgtggtcgt ctctaaagag gaatcggagg gtcgcaccat cacccgcatc
      121 gggcgcatcg aaggagagga acgccgcaga gagctggcca ggatgctctc tggacgcatc
      181 gacgaggcct ctctcgccca cgccaccgag ctactcgtca gcggcaggta atgcagagct
      241 gcccgcgaaa agccagcgcc agaagctgat agccgaaggc tgatagctag aagctatctc
      301 caaagagcgg tgtgtatggc cggaccactc caggccatat tggtatcggt gccggtgatg
      361 tcgcgcgggc tgatccactc ttccgcgagg gcaatccgca ccgcctccgc acgcgaggaa
      421 gccccgatct tggggaatat gtttgccagg tggcgcttgg cggtcgcccc cgaggtgtac
      481 agggagccgg cgatctggtc gttgctcatg tcgcgggcga ccatgagcag cacctccagc
      541 tcgcgttcgg tgaggccgtc ggccttctgc gggcctctga agaccttggg tggaacgacc
      601 attacggcgt catcgtcggg gccaacaggg ctttgagcgc cctgcggacc gccgagatta
      661 gctcccccat gctggcgctc ttcaccagaa aggccgaggc cccgagccct tcaaagctcg
      721 cgcacgagcc cgggttcgtc gtgcatggta actatagata cccttggcgt ggaggcgtcc
      781 tcgggagggt cattcatcat gagtcgcatg acctcctcag cacccatgag gggcatgtcc
      841 acatccagca gcaccacgtc gggtacgagc ctcttgatca tggccacggc ccgctcgccg
      901 atttcggcct cgccaaccac ctctatctcc tcggctgttc ggagcatctc cacgacgccc
      961 tgcctgaaca tggcgtggtc gtcggccacg agcaccctga tggcaggctt atctctggtg
     1021 gcatcctcgt tcacggcgta tcaccccctc ctgcaagaga tcgagtccag gttatcgtag
     1081 cttcccggtg tctatgcgta gacagccgac cctcgctccc acacctcctg atacccggcg
     1141 ccgaaaaccg gactctgctt acggggataa agatgcttgc ctgctatttt cttttctccc
     1201 caaacgtcgt atgatagaga tccatgcagg tgcgtcggcc gagaagtttg cgggaagcga
     1261 ggatcccgtg tctcaccgcc gacgctcctc tcttttttcc gggtcgtgga gaaggttcgg
     1321 gcgtctttcg ggttccatta tgggggtgta gatgagcgag accaagtata tcttcgtgac
     1381 gggcggcgtc gtttcgtcga tcggcaaggg gacgagcgcg gcggcactgg gcatgctgct
     1441 aaagcgccgc ggctacaggg tggtgctgca gaagttcgac ccctatataa acgtggaccc
     1501 cggcacgatg aacccctacc agcacggaga ggtcttcgtc acggaggatg gcgccgagac
     1561 ggacctggac ctcggccact acgagcggtt cctggatgag aaccttggca agctctccaa
     1621 cgttacgacg gggagcgtat actgggaggt catagcccgc gagcggcggg gggactacct
     1681 cggggccacc gtgcaggtca tcccgcacat taccaacgag atcaagaacc gcataggacg
     1741 cctcgggcag gacaaggaca tcgtcataac agagatcggc ggcacggtcg gcgatatcga
     1801 gagcctgcct ttcctggagg caatcaggca gttcagaaac gacgttggcc gcaagaacgt
     1861 gctctacgtc cacgtctcat acgtgcccta catcgaggcc gcaggagaac tcaagaccaa
     1921 gccgacccag cactcggccc agagactgcg cgagatcggc ataagccccg acgtcctgat
     1981 ctgccgcgcc gacaggccca tcagcgagga catcaggaga aagatagcgc tcttcgcaga
     2041 taccgagctg gactcggtca tagcggcgga agacgccccc aacctctacg ccatccccct
     2101 gagcctgcac gaggagggcc tcgacgacct ggtgatcgag aagcttggcc tcgacgctcc
     2161 caacgccgac ctggaggagt ggcgacgcct ggtctcgcgc atgctcgagg cgcgccgaac
     2221 ggtcaaagtg gcaattatcg gcaagtacat caagcttcag gacgcctacc tctcggtggt
     2281 tgaggccctg gcccacgccg gtgggtccca cgacctgaag gtggagcttg actgggtcga
     2341 ctccgagcaa ctggtcgatc gggaatcgac cgaacaacgg ctcgccggcg tagacggggt
     2401 gcttgtgctg cctggcttcg gccacagggg tgctgagggc aagatcgagg cagcccgcta
     2461 cgccagggag acaggcaccc cctacctggg cctctgcctg gggatgcaga tagaggtgat
     2521 cgagttcgcc cgcaacgcgg cggggctcga gatggccaac tccaccgagt tcgagcagga
     2581 tacaccccac ccggtgatag acatcatgcc agaccaggtg ggggtggaga tgggcggtac
     2641 gatgcgcctg ggccactggc cctgccagat agtgccgggc accctggccg aggaggtcta
     2701 cggcaccgag ctggtcggcg aacgacaccg ccaccgctac gaggtgaaca atgcctacag
     2761 ggacgccctc gagaacgccg ggatggtctt ctcaggcacc tccccggacg gcaggctggt
     2821 ggagattgca gagatagcgg accacctgtt ctttataggg agccagttcc acccggagtt
     2881 caagagccgt cccctgcgcc cacacccgct gttctccggc ttcgtcgggg cctgcaaggc
     2941 ggcttccgac gagccggtcg acgacacggt tgtcgtgccc gaggagaggg cggcccgctg
     3001 agcgaaacac cccaggcgcg cgggctgctc ggcaccgggc cgatgccagc ggccgtgcgt
     3061 cctacccccg acccttctgg caccgtcgcc atccgctccg ccggcctcgt ggagttcagg
     3121 gcgaagctcg gttaccggag cgcggatgag gtaccacgtg ggcccacgct gggagccccg
     3181 ccctccggcg cgcgcctctc cgtcgttgag acggatgatg gggtgacgcg tgagatactg
     3241 ggcgagatcc tggccctctc cgaagccgac ctcggcagag cccttgaggg cgtgttgcgc
     3301 tccctgacca tcgccgcggc gctgacagac gccgttctcg agaccagcgc cgcccgtgtg
     3361 gtgccggtcc gtaccgcgcc cgccgggccg cttcaaacgc tcgcgctcga cgttttggag
     3421 gcgggcttcc gggtgcgctt cgccccgacg tggacaccgc tgtctgaagg cgaactcggc
     3481 ctgggtgtcg ggaacgacga agggaacctc agggccatct tcggggccca gctcccctgg
     3541 agcctggttg ggaccggggc ggtctccggt ggcagcaccg cgcgatactt cggtgaggag
     3601 gaactatgag gatcggggtg ccgagggaga ggaccccggg ggagagcagg gtcgccctgg
     3661 ccccccacgc ggtctacgag ttgacccgcg ttggccacag ggtctttatc gaagctggag
     3721 cgggggaggc ctcgagcatc tctgacgaag cgtacgatca ggcgggcggg cgcatcgtgg
     3781 acgacccggc caggctctac ggcgactcgg agattgtggt caaggtctac gggcccctgg
     3841 aggaggagtt cgcgtacctg agggagggcc tcgtcctcgt ctctttcctc tccctgccgg
     3901 tgaaccccgg cctgatggac gccctgctcg actcccgttg cgtggccttc gccatggaga
     3961 ccatccgtga cgaacgcggg ggcctcccgg tgctcacgcc gatgagcgag atcgccggcc
     4021 gccttacccc gcagataggc gcccactacc tgcagcgccc cgcggggggg cgcggcatcc
     4081 tgctcggcgg ctcgaccggc gtgaggccgg ggcgcgtggt catcctgggg tgcggcatcg
     4141 tgggctcggg cgccgcaagg gtggcgagcg gccttggggc ctccgtggtg gtcatagata
     4201 gggacctgga tcgcctccgc cgcctggagc agttccggct gcccaacgtg accacgctgg
     4261 cgtcgagcag cctcggcatc agggacgccg ttctgcgctc ggatctgctc ataggggccg
     4321 tgcatgtggt cggtgcccgc acgcccgccc tggtggcccg cgatacagtc tccgagatga
     4381 gggacgggag cgtgatcgtg gacgtggacg tggactacgg tgggtgcgta gagacctccc
     4441 gcccgaccac cctgtccgac ccgaccttcg tggagtatgg cgtgacccac tactgcgtga
     4501 agaacgtgcc agccgccgtg cccgttacgg ccacgcgggc cctgagcaac gccctgctgc
     4561 cgctcatgcg gcgtctggca ggcgttggcc tgccagacgc cctcgccgcc gacgagggcc
     4621 tggcgcgcgg ggtagtcgtg gccgagggcc gggtcgtcga cgccgtgctg gcacgcgcga
     4681 cgggcacgga gcacaaccgg ctctcctctg tcttgccgct caactcggag gccaggtgac
     4741 cttcgagccc cagggacgct cgctcgcgga cttcacggtc gagctcgacg tctactcggg
     4801 cccctacgag tggcttctgg ccctcatcct caaggacgag ctggagatct tcgagatacc
     4861 cctgcgcgag ctggtcgagt cctacgtcgg cgcgagggag cgggaccccg ccgggggcgg
     4921 cctggagcgc gacacggact ttgccggctc ggcctcggcc ctcgtgctgc tcaagagccg
     4981 tacgctctcc ccagccctcg accccgacgc cgagggtgac gaggacgaga ccctctcgcc
     5041 gcaggagctt gccgagaggc tcacggagta cctcaagatc cgtcgcgccg ccgaacacct
     5101 gcgcgcacgc ttcgcggcga acgcgggcca ccacccctct tc
//
LOCUS       DBNGVD010000617         5006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4004903, whole genome shotgun sequence.
ACCESSION   DBNGVD010000617 DBNGVD010000000
VERSION     DBNGVD010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4004903"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..870)
                     /locus_tag="AC16CM_16385"
     CDS             complement(298..870)
                     /locus_tag="AC16CM_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3141462.1"
                     /translation="MTATTTDHHTGIPEPNVTRFLFADTRMAPVWLILRLWLGYEWLT
                     AALGKWTEGGWVGAGAGGAVKGYAQGAMAQTAGDHPQVTGWYASFLENVVVPNAALFS
                     YLVILGETLVGIALVLGAFTGIAAFFGVFMNASFLFAGTAGANPLMALVAILLVLAWR
                     VAGWWGLDRLILPAIGVPGAAGTLFKRRVR"
     gene            complement(1375..1743)
                     /locus_tag="AC16CM_16390"
     CDS             complement(1375..1743)
                     /locus_tag="AC16CM_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3141463.1"
                     /translation="MVDSGVDHSGQVCATYQRAAEIIGRRWSAAILRALLGDATQFCQ
                     VRAAVPDVSNRMLSERLREFEAEGIVQRLVIPERPVRVEYRLTEKGRALEGVVDAVAA
                     WAEEWVSIEETGDEQAAGSS"
     gene            1807..2241
                     /locus_tag="AC16CM_16395"
     CDS             1807..2241
                     /locus_tag="AC16CM_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3141464.1"
                     /translation="MKSNYLGGMMIGIALFVLRLVLGIIFIAHGAQKLFGSFGGPGIS
                     GFAGALEHMGVRPARPMAILAGLGEFVGGILMILGFLTPLAALALIAVMIVAIVTVHL
                     KNGFFATNGGYEFNLALAGIALTLLVVGGGAYSIDSYLGIFW"
     gene            2474..2794
                     /locus_tag="AC16CM_16400"
     CDS             2474..2794
                     /locus_tag="AC16CM_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HIR3141465.1"
                     /translation="MSEGSGRAEMERRIVQRSVEDDAFRQRLIEDPKAAVEQELGSRL
                     PEEVRVVTVEESADTFYVVLPSTSTEVAEGGELSDQELQSVAGGWEYYTMGLICTSDE
                     QCQS"
     gene            complement(2913..3857)
                     /locus_tag="AC16CM_16405"
     CDS             complement(2913..3857)
                     /locus_tag="AC16CM_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3141466.1"
                     /translation="MPRRPIDITIPDLYGKRAVITGASDGIGLGLATRLAAVGAEVLL
                     PVRNKRKGEAAIATIRQKVPNANTTLRELDLSSLASVSALGETLREEDRPIHILINNA
                     GVMTPPGRQTTADGFELQFGTNHLGHFALVAHLLPLLCAGQARVTSQVSVAANRGAIN
                     WDDLNWERSYDGMKAYSQSKIALGLFGLELDRRSQANGWGITSNLSHPGIAPTNLLAA
                     RPEVGRDKDTTSVRMIRALSTRGILAGTVQTAQLPALYGATSPDAEPGGFYGPRGLWH
                     MGGPPGQQRLYSRLRSEEEAERVWRASGELTKVPFPKG"
     gene            4149..4991
                     /locus_tag="AC16CM_16410"
     CDS             4149..4991
                     /locus_tag="AC16CM_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3141467.1"
                     /translation="MEIDRAGLAVFLRHRRESLQPEDVGLPRGQRRRTIGLRREEVAS
                     LCHMSTDYYSRLERERGPRPSEQMIASIAQGLRLSLDERDYLFRLAGHNPPTRGTVSE
                     HISPGLLRIFDRLHDTPAEIVTELGETLRQTAPGVALTGDLAAYRGPARSIGYRWFTD
                     PATRQLYDPSDHPFLTRMFVSGLRGVVTLRGPGSRAAHLRDLLLARSDEFREVWDQHE
                     VGIRPDEVKHFIHPELGALELNCQTLLDPGQSHSLLVYTAVPGSESYEKLQLLSVIGT
                     QALR"
BASE COUNT          872 a         1550 c         1649 g          935 t
ORIGIN      
        1 gtaagagtgt ggccgtcttc ttcgccaaca gggacgaggt ccgcggcaac tgccggtaat
       61 cccctaatga ggtgtgcggt gcagaagaag gccgtcaaga cggaactccg tgaacaactg
      121 ttccgaatac tccggtgaat cagggaacaa ggagaggccg ataccagcca cgccccgacc
      181 cggatcctgg tgcgttggta gtctattttg ggcctcctgg cgcacacatc ccgggctgcc
      241 tctccgggag gcgtttctca tccagagagg ctcccgttcc cagcagctac tgtccgttca
      301 tctgacacgc ctcttgaaca gagtccccgc ggccccggga acgcctatcg cgggcaggat
      361 caagcggtcg aggccccacc atccggccac gcgccacgcc aggacgagca ggatggctac
      421 cagtgccatg agcggattgg cccccgccgt tcccgcgaac aggaagcttg cgttcatgaa
      481 caccccgaag aaggccgcta tgccggtgaa ggccccgaga acgagggcga tcccgaccag
      541 ggtctcccca aggataacca ggtaggagaa cagggcggcg tttggcacaa ccacgttttc
      601 gaggaagctg gcgtaccatc cggtgacctg tgggtgatca cccgcggtct gggccatggc
      661 gccctgggca tagcccttta cagcaccgcc cgcccctgcg ccgacccagc cgccctctgt
      721 ccacttaccc agggcagccg tgagccactc gtagccgagc cagagcctca ggatcagcca
      781 gaccggtgcc atcctggtgt cggcgaacag gaagcgggtc acgttcggtt cgggaatgcc
      841 tgtgtggtgg tccgtcgtgg ttgctgtcat gattctcctt tgtgagtgcg gagggtcgga
      901 cctccggccc acgtaacctc tgtgcgtgtc ggcgagtgct tacctttctt catgggtcat
      961 cgtctctgtc tgctcgtcct cgaatgcctc ctcgtaggtc cccataccca agtgaggcta
     1021 acgcggcagg ggcgtctcgc catgacacag attgatgctc caactggcaa agattgatca
     1081 acatatccag ggtgttacag gtcgttgggc aacggtcgcc aggcatagcc gctcttcagg
     1141 actgcaggat ggcgcccggg acctcttgcg tgacgtgtag gtaacgaagc ctgagagcta
     1201 caaggtgggg aaggtgggcc cccagacagc tctattctaa gacggttctc gaacgatgtt
     1261 ccaggacgtt cagccaggcg atgggccagc ccgttactgc ttgccacgag cggtcgagaa
     1321 gcgccttcgg cccgtgatct ctacataccg cgacccgctc tggaacgacc gaatctagga
     1381 gcttccggcg gcctgctcgt ccccggtttc ctcgatggag acccactcct cggcccacgc
     1441 ggccaccgcg tcgaccacgc cttcgagcgc ccttcccttc tccgtgagcc tgtactccac
     1501 ccgcacggga cgctccggaa ttaccaacct ctgcactatg ccctccgcct cgaattccct
     1561 caggcgttcc gagagcatgc ggtttgagac gtctgggacc gcggcccgta cctggcagaa
     1621 ttgcgtggca tctccaagca gggcacgcag tatcgccgcg ctccagcggc gccctattat
     1681 ctccgccgcc cgctgatagg tggcacagac ctgccctgag tgatccacgc ccgagtcgac
     1741 catagcctcc tcactctacc accaacatcg cattgacgta tccaacttac tgatgtatat
     1801 ctggttatga aaagtaacta cctgggaggt atgatgatcg gcattgctct gttcgtgttg
     1861 cggttggtac tggggatcat cttcatcgcc cacggcgcgc agaagctctt cggcagcttc
     1921 ggcggacccg ggatatccgg gtttgcgggg gcgctggagc atatgggggt gaggcccgcg
     1981 aggcccatgg cgatcctggc cggcctcggg gagttcgtcg gcgggatact gatgattctg
     2041 ggctttctga cgcccctggc ggcgctcgcc ctcatcgcgg tgatgatcgt ggcgatagtg
     2101 acggtacatt tgaagaacgg cttcttcgcc accaacggag gctacgagtt caacctggcg
     2161 ctcgctggca ttgcgttgac cctcctggtc gtgggcggag gagcctacag catcgacagc
     2221 tatctgggta tcttctggta gacccgacgg ccacggcggc cgtagcgctc gtgatgtcgg
     2281 accgggggtg ctttatgcaa ccgacgttcg agaagggtcg ttctccgagc ttcctctaga
     2341 gttcgccgcc taccggtcct ctatatggcc cgagggtggt tggcatggga tactagcccc
     2401 ttcgcctccg accacgggca tgagaggccc gggaggcggg tgctgcggaa agaaggaaag
     2461 gggaagggcg ctcatgagcg aagggtcggg cagggcggag atggagcgca ggatagtcca
     2521 gaggagcgtg gaggacgacg ccttcaggca gaggctcata gaggacccga aggcggccgt
     2581 ggagcaggag cttggctcgc ggctgcccga ggaggttcgg gtggtcaccg tggaggagag
     2641 tgcggacacc ttctacgtcg tacttccaag cacctcgacg gaagttgcgg aaggcggaga
     2701 gctttcggat caggaacttc agtcggtggc cggcggctgg gaatactata cgatggggct
     2761 tatctgtacc tctgatgagc aatgccagtc ctagtcgttt tcatctgatc cgggtgcccc
     2821 cgtccccgag gcggcggggg gcatgtgacg tagccgagat cgtgcgacgc caggcggaaa
     2881 tcctgggtga gagagcgtcc gactcgagag cctcagcctt tgggaaaggg aaccttcgtg
     2941 agttcccctg aggctcgcca gacgcgctcg gcctcctctt cgctgcgtag gcgagagtag
     3001 agcctctgct gcccgggagg gccgcccatg tgccagagcc cgcgcgggcc gtagaacccg
     3061 ccaggctcgg cgtcggggga ggtggcaccg tagagggcgg gaagttgcgc ggtctggact
     3121 gtccctgcga ggatgccgcg ggtggataga gcccgaatca tgcgcactga cgtggtgtcc
     3181 ttatcgcggc ccacctcggg gcgggcggcc agcaggttcg tcggtgcgat gcccgggtgg
     3241 gagaggttgc tcgtgatacc ccaaccatta gcctgactgc gccggtccag ctccaggccg
     3301 aagagcccga gggcgatctt ggattggctg taggccttca taccgtcgta ggagcgttcc
     3361 cagttcaggt cgtcccagtt gatggcaccc ctgttcgccg cgacgctgac ctgcgaggtc
     3421 acgcgtgcct ggccggcgca gagcaacggt agcaggtggg ctaccagggc gaaatggccg
     3481 aggtggttgg tgccgaactg aagctcgaac ccgtcggcgg tcgtctgccg gccaggcggg
     3541 gtcatgacgc cggcgttgtt gatgaggatg tggatcggac gatcctcctc tcgcagggtc
     3601 tcgccgaggg ctgagacgga ggcgagcgag gacagatcga gctcgcgaag ggttgtgttc
     3661 gcgttgggga ccttctggcg gatcgtggcg atcgccgcct cacccttgcg tttgttccga
     3721 acgggcagga gtacttctgc cccgaccgcg gcgagcctcg tggcgagtcc caggccgatg
     3781 ccatcgctcg ctcccgtgat tacggcgcgc ttgccgtaca ggtcgggtat ggtgatgtcg
     3841 atgggtctgc gtggcatgtg ttcgctcctc agagtcgtgt catcgcctcc accgtcgtgg
     3901 aagatgtcta catggtcgtt gaagatggcg gcgttattca gggcttctcg atcccctgat
     3961 acggaaggcc aggttaccgc gggacccgcg tagggagaga cgtgagcccg gccggtggct
     4021 attggatcgc cccgctggcg ctccgggtag cgactggttc cgggaagggg ggatcgtcag
     4081 gccgtggatg cgcagatgcg cgggcggtat aagtaggcag gaacgaagcc gcatcgaagg
     4141 aggcacgcat ggagatcgac cgggccggac ttgcggtctt tctacggcat cgccgggagt
     4201 cactgcagcc cgaggatgtc ggcctcccgc ggggacaacg ccgcaggacg atcgggctac
     4261 ggagggagga ggtggcctca ctctgtcaca tgtccaccga ctactactcg cggctcgagc
     4321 gggagcgcgg ccctcggcca tccgagcaga tgatcgcctc catcgcgcaa gggctccgtc
     4381 tctcgctaga tgagcgtgac taccttttcc ggctcgccgg acacaaccca ccaacgcggg
     4441 gcacggtcag cgagcacatc agcccgggcc tgctgcgcat cttcgaccgc ctgcatgaca
     4501 ctcctgcgga gatcgtcacc gagctcggag agacgctgcg gcagaccgcg cccggggtcg
     4561 cgctcaccgg cgacctggcc gcctacaggg ggccggcccg aagcatcggc taccgatggt
     4621 tcaccgaccc cgccacgcgg cagctctacg atcccagcga ccatccattc ctcacccgca
     4681 tgttcgtctc gggactgcgt ggggtcgtaa cgctgcgagg ccctggttcc cgggcggcgc
     4741 acctccgcga cctgcttctc gccaggagcg atgagttccg ggaggtgtgg gaccagcacg
     4801 aggtcggcat tcgtcccgac gaggtgaagc acttcatcca ccccgagctc ggcgcactgg
     4861 agttgaactg ccagacgctg ctcgatcctg gccagtcgca ttcgctgctc gtctacaccg
     4921 ccgttcccgg cagtgagagc tacgagaagt tacagcttct ttccgttatc ggaacgcagg
     4981 cacttcgcta ggggccacgc ccacaa
//
LOCUS       DBNGVD010000618         2413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_4734544, whole genome shotgun sequence.
ACCESSION   DBNGVD010000618 DBNGVD010000000
VERSION     DBNGVD010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_4734544"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16CM_16415"
     CDS             <1..552
                     /locus_tag="AC16CM_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HIR3141468.1"
                     /translation="NYGRLGDDQQRHYTDANARLQLIPDEQPGAMPEIAPSPDYGRSI
                     DRPFNERAMVLQAWGAYGTIWPVVHQQLGVRPDMGRDRMTVVPQVPPGSPGLSGENIR
                     LGDGTVSVSAEHDGNTYTTEVSRFVTARLTIGHTIPRGADVAAVEVNGDKTEDYRVRE
                     TNRGKEVLVDTTAADVELVVRTE"
     gene            642..1646
                     /locus_tag="AC16CM_16420"
     CDS             642..1646
                     /locus_tag="AC16CM_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3141469.1"
                     /translation="MSKTRPMIAVLVLASVAAALVAALVVAAAPVRAEKSATRSTASS
                     LPNAPVINIGHRGASGYAPEHTFASYDLAIRLGADYIEQDLQLTRDGVLVVMHDKTLN
                     RTATAPEGVPARYCRGPVINRTLRQIKQCDVGSWFNEEYPEYAKARYVGLKIPTLEQV
                     FRRYGKEANYYIETKNPDAAPGMEEELLRLMKKYELIRPAVRDWQVLIQSFSPESLKK
                     IHRLRPSLPLVQLYFAGSSKTVRTDLDEAKAYAVGIGPDQKDVDARLVSAAHRRCLVV
                     HPYTVNETPDMRHLISLGVDGMFTNFPDRLDAVLGSRAADAMVAAQRSAEAYRTCRGG
                     "
     gene            1869..>2413
                     /locus_tag="AC16CM_16425"
     CDS             1869..>2413
                     /locus_tag="AC16CM_16425"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3141470.1"
                     /translation="MGIGGNWDRRVSRRKFLGVGGLSAAALMLNPTGVSAQESTRAGY
                     GPTQPDPRGRLDLPEGFRYRVISEEGSRLSDGTPVPGDHDGMVAVRGDGDTTILVRNH
                     ELRLGDANPLEGTRPYDADQIGGTTALVVGSDRRVIRDYVTSSGTVNNCAGGGTPWGT
                     WLTCEEDRTTIGRRDHGYVFEV"
BASE COUNT          480 a          778 c          801 g          354 t
ORIGIN      
        1 aactacggcc ggctcggcga cgaccagcag cgccactaca cggacgccaa cgccaggctc
       61 cagctgatac cggatgagca gccgggcgcc atgcccgaga tagccccgtc cccggactac
      121 ggccgctcga tcgacaggcc gttcaacgag cgggcgatgg tcctgcaggc ctggggcgcc
      181 tacggcacga tctggccggt cgtccaccag cagctcggcg tccgtcccga catggggcgt
      241 gacaggatga cggtcgtccc gcaagttccg cccggctcac ccgggctctc cggtgagaac
      301 atccggctcg gtgacggcac cgtaagcgtc tcagccgagc acgacggcaa cacgtacacc
      361 accgaggtgt ccaggttcgt caccgcaagg ctgaccatcg ggcacaccat cccacgtggg
      421 gccgacgtcg ctgccgtcga ggtgaacggc gataagaccg aggactacag ggtccgcgag
      481 accaacaggg gaaaggaggt cctggtagac acgaccgccg ccgacgtgga gctggttgtc
      541 aggacggagt aagagtttgc cggggagggg gcggatcatg atccgccccc tctcgcatgt
      601 ccgaacaggg ccgcggccca cgacgaacga ggagagagcg agtgagcaag acgagaccaa
      661 tgatagccgt gctggttctg gcgtcggtag ccgcggcgct cgtcgccgcg ctggtggttg
      721 ccgcagctcc ggttcgggcc gagaagtccg cgacgcggtc gaccgccagc tcgttgccga
      781 acgcccccgt gatcaacatc gggcatcgcg gggcttccgg atacgccccg gagcacactt
      841 tcgcgtccta cgaccttgcc atcaggttgg gcgccgacta tatagagcag gacctgcagc
      901 tgaccaggga cggggtgctc gtcgtcatgc acgacaagac cctcaaccgc accgcgacgg
      961 cgccagaagg ggtgcccgca cgctactgca ggggccccgt gatcaacagg acgctcaggc
     1021 agataaagca gtgcgacgtg ggtagctggt tcaacgagga gtatccagag tacgcgaagg
     1081 ccaggtacgt ggggctcaag atcccaacgc tcgagcaggt cttcaggcgc tacggcaagg
     1141 aagcaaacta ctacatcgag accaagaacc ccgacgcggc ccccggcatg gaagaggagc
     1201 tgctgcgcct gatgaagaag tacgagctca tcaggccggc cgtcagggac tggcaggtgc
     1261 tcatacaatc cttcagcccg gagagcctca agaagatcca ccgtctcagg ccctcgctcc
     1321 ctctcgtaca gctctacttc gccggtagca gcaagaccgt acgcaccgat ctcgacgagg
     1381 cgaaggccta cgccgtcggc atcggcccgg accagaaaga cgtcgacgcc aggctcgtct
     1441 cggcggccca caggcgctgc ctcgtggtcc acccctacac cgtgaacgag accccggata
     1501 tgaggcacct catctccctc ggcgttgacg gcatgttcac caacttcccc gaccgcctcg
     1561 acgccgtgct cggctccagg gcagccgacg ccatggtcgc ggcccagagg tcagcggaag
     1621 cgtacagaac ctgccgggga ggctgaggag gtccgaggct atccccgggc cacggggcat
     1681 acgccggggg tgagaacaga gggagatcgg ccccccggga agggccgatc tccctcttcc
     1741 gtctcgacgt cgggcgatgg tgtgccaaga gttaactcag aaattacctt cttgtaacag
     1801 aacagccgtg tcctgctgct aatctccctc ctgttcgtgg agtgtgggca aacagggagg
     1861 gtggcgtctt gggcatcggc gggaactggg accggcgtgt aagcaggcgg aaattccttg
     1921 gcgtgggtgg cctgagcgcg gctgcactga tgttgaaccc gactggggtg tccgcccagg
     1981 agagcacccg ggccggctac ggtccgaccc agccggaccc cagaggcagg ctggacctgc
     2041 cagaaggctt caggtaccgc gtgatctccg aggaaggctc gcggctctcc gatggcacgc
     2101 cggttccagg cgatcacgac gggatggtgg ccgtcagggg tgacggagac accacgatcc
     2161 tggtgcgcaa ccacgagttg cgactcggtg atgccaatcc tctcgaaggc acgagacctt
     2221 acgatgccga ccagataggc ggcacgaccg ccctcgtcgt cgggtcggac aggagggtga
     2281 tccgggacta cgtcacctcc tctggaacgg taaacaactg cgctggtggc ggcaccccgt
     2341 ggggcacgtg gctgacctgc gaagaagacc gcacgacgat cgggagaaga gaccacggct
     2401 acgtgttcga ggt
//
LOCUS       DBNGVD010000619         4415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_726416, whole genome shotgun sequence.
ACCESSION   DBNGVD010000619 DBNGVD010000000
VERSION     DBNGVD010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_726416"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /gene="otsB"
                     /locus_tag="AC16CM_16430"
     CDS             <1..308
                     /gene="otsB"
                     /locus_tag="AC16CM_16430"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006552154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3141471.1"
                     /translation="DDLKMTPGKMVYEIQPKIDWDKGKAVLYLLETLELDRDDVVPIY
                     VGDDVTDEDAFGAISGMGIGVFVGGADDPDSAGRSTAADYMLDDVQEVERFLNGLAR"
     gene            606..3026
                     /locus_tag="AC16CM_16435"
     CDS             606..3026
                     /locus_tag="AC16CM_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR3141472.1"
                     /translation="MKNWTLAYDSFDADEEGLREALTSTGNGYFCTRGAAHWADADKV
                     HYPGVYVHSGYNRETTIMAGRPVLNEDLVNFPNWLVLKLRIEDDAEPVDFDNVELLDY
                     NHELDIRQATMIRNMRFRDGEGRETSLESRRFVSMSDMHHAGIEWTITPENWSGKAEI
                     ITAVDGRVTNGGVPRYQQLEGRHLDSVSPRTFGPEIIALETRTRQSRIYVAQAARTVV
                     YGQDAKLLVDRHLYQLQDYIHQVLAFEVEEKVPVRVEKMQSFYTSRDHAINEPLVNAG
                     KAVARCGTFAESHDRHVRAWEALWESCDVVVPKHDRVQLLLRFHIYHVLQVCSPHTAD
                     LDAGVPSRGLNGEAYRGHVQWDELYVYPFLNFRLPEVTRALLMYRYRRLGEARAAARE
                     AGYEGAMYPWQSGSDGQEETQVVHLNPESGSWDPDLSHNQRHVGAAIFYNIWQYYRTT
                     GDFEFLLDCGAEMMLDIARFWASMVHHNPERDRYEIHGVMGPDEFHEQYPGSEEEGLN
                     NNAYTNVMVAWISKTTLEMLDLLPEKRRDALRAATGLTDDELRLWDEMSRKMFVPFHD
                     DGIISQFEGYEELEELDWDAYREQYDNIQRLDRILKAEEDDPNRYKLAKQADTLMLFF
                     LFPARELEEIFERLGYEYGPETASKNVSYYAERTSHGSTLSFIVHAHILADVDPEASW
                     DMFLTALESDIGDIQGGTTQEGIHMGVMAGTLDLIQRGYLGSTFRDGALYFDPKLTDK
                     LEGLSFSMRFRDTPLSVNLEAGKLKVSVEHGGGGGAIKIGVGEDVQTIEPGEAHDFDL
                     SSATASTT"
     gene            3054..3746
                     /gene="rpiA"
                     /locus_tag="AC16CM_16440"
     CDS             3054..3746
                     /gene="rpiA"
                     /locus_tag="AC16CM_16440"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIR3141473.1"
                     /translation="MKVAPEILRSTAHAAVDARLESGMTVGLGSGTAASLAVERIGEL
                     LSSGELEDVRGIPTSEKTARLAREVGIPLLDLSASGPDVTIDGADEIGEGNLSLIKGH
                     GGALLREKIVASCSYLVVVADASKLVNELGRGSIPVEVEPFGWEATVAALSSLGCEAT
                     LRRAPNNPELPFVTDGGHFTVDCMFGPMPDPASLEAEIRRIPGALECGIFVDLARVAY
                     VAHEAGTDIMEA"
     gene            3913..>4415
                     /locus_tag="AC16CM_16445"
     CDS             3913..>4415
                     /locus_tag="AC16CM_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3141474.1"
                     /translation="MGLFSDLDDKLVGGPSILAADFARLADDVSKARDGGAAFIHFDT
                     MDNHYVPNLSFGPPVAASLASHTDLPIDVHLMVDNPDNLIPDFLDAGVTSISVHPEAV
                     THLHRTLAVIRDGGAEAGVALNPTTSPETLEWTLPYLDFILVLSVDPGFGGQSFLPEA
                     AEKIRRL"
BASE COUNT          856 a         1353 c         1443 g          763 t
ORIGIN      
        1 ctgacgattt gaagatgacg ccgggcaaga tggtctacga gatccaacca aagatcgact
       61 gggacaaggg gaaggccgtg ctctaccttc tcgaaaccct cgagctcgac agggacgacg
      121 tggtgcctat ctacgtcggt gacgacgtca cggatgagga cgcgttcggg gcgatatccg
      181 gtatgggcat cggtgtcttc gtgggcgggg ctgacgaccc tgactccgcg ggaaggtcca
      241 cggcggcgga ttacatgctc gacgacgtgc aggaggtgga gcgatttttg aacgggcttg
      301 cgcgctgagg tttcggcctg gcgtcaggcc cgggagtcgt gctagacgga ggagtgtgtg
      361 gagactattt cgagatccgg gtggtcgcaa gtgccgggtt gcaagcagtt gggagagtgg
      421 ggggtggtcg acgtcgccgg atgctgcgca atctgtcact cggctgaccg gtacgccgga
      481 gacctctcgc ctgggccctg cctggcaacg cttccgggcg gcggttcggc gctcgtgtgc
      541 tgcgccgcca gaaagcagtt gcgcgaggta gccagaaccg aagccggtat accaggaggt
      601 acagagtgaa gaattggacg ctagcctacg attcgttcga cgccgacgag gaggggctgc
      661 gagaggcgtt gacctcgacc ggcaacggct acttctgcac gcggggggca gcccactggg
      721 ccgacgccga caaggtgcat tacccaggag tctacgtcca cagcggttac aaccgggaga
      781 cgaccatcat ggccggccga ccggttctga acgaggacct ggtcaacttt ccaaactggc
      841 tggtcttgaa gctcaggatc gaagacgacg ccgagccggt cgatttcgac aacgtggagc
      901 tgctcgacta caaccacgag cttgatatcc ggcaggcgac catgatccga aacatgcgct
      961 tcagggacgg cgagggccgg gagacgtccc tggagagccg ccggttcgtg agcatgtccg
     1021 acatgcacca tgcgggaata gagtggacga ttacccccga gaactggtcg gggaaggccg
     1081 agatcataac ggcggtggac ggacgggtca ccaacggggg cgtcccacgc taccagcagc
     1141 tggagggtcg tcacctggac tcggtctccc cgcgcacgtt cgggcccgag atcatcgccc
     1201 tcgagactag aacccgccag tcgcgcatct acgtggccca ggccgcccgc accgtcgtct
     1261 acgggcagga cgccaagctc ctcgtcgacc ggcacctcta ccagctgcag gactacatac
     1321 accaggtgct cgccttcgag gtcgaggaga aagtgcccgt gcgggtggag aagatgcagt
     1381 ccttctacac ctcgcgcgac cacgccataa acgagccgct ggtcaacgcg ggcaaggccg
     1441 tcgcgcgctg cggcaccttc gccgagtcgc acgaccggca cgtacgggcc tgggaagcgt
     1501 tgtgggagtc ctgcgacgtg gtcgttccga aacacgaccg cgtccagctc ttgctccggt
     1561 tccacatcta ccatgtcttg caggtctgct cgccgcacac tgccgacctg gatgccggcg
     1621 taccctcgcg gggtctcaac ggcgaggcat accggggaca cgtgcagtgg gacgaactct
     1681 acgtctatcc cttcctgaac ttcaggctgc cggaggtaac ccgcgcgctg ctcatgtacc
     1741 gctacaggcg tctgggcgag gccagggccg cggcgcgcga agcgggttat gagggggcca
     1801 tgtacccctg gcagagcggc tccgacgggc aggaagagac ccaggttgtt cacctcaacc
     1861 ccgagtcggg aagctgggac ccggacctca gccacaacca gcgccatgtc ggcgcggcga
     1921 tcttctacaa catctggcag tactacagga ccacgggaga cttcgaattc ctgctcgact
     1981 gcggggccga gatgatgctc gatatcgcgc gcttctgggc gagcatggtc caccacaacc
     2041 cagagcgaga ccgctacgag atccacggcg tcatggggcc agacgagttc cacgagcagt
     2101 acccgggctc ggaagaagag ggcctgaaca acaacgccta caccaacgtt atggtcgcct
     2161 ggatctccaa gacgaccctg gagatgctgg atctcctacc cgagaagcgt cgcgacgcgc
     2221 tgcgcgctgc cacgggtctt accgacgatg agttgcgtct gtgggacgag atgagccgca
     2281 agatgttcgt gccgttccac gacgatggga tcatcagcca gttcgagggc tacgaagagc
     2341 tcgaggagct tgactgggac gcctacaggg agcagtacga caacatccag cgcctcgacc
     2401 gcatcctgaa ggccgaggaa gacgacccga accggtacaa gctggccaaa caggctgata
     2461 cgcttatgct gttcttcctc tttccggcgc gagagctgga ggagatcttc gagcgcctcg
     2521 gctacgaata cggtcccgag acggccagca agaacgtctc ctactacgct gagaggacca
     2581 gccacggctc gaccctcagc ttcatcgtgc acgcgcacat cctggccgac gtcgacccgg
     2641 aagcttcctg ggatatgttc ttgacggccc tggagagcga tatcggtgac attcagggcg
     2701 gtaccacgca agagggcatc cacatgggcg tcatggctgg gaccctggac ctgatacaac
     2761 gcggctacct gggaagcact tttcgggatg gagcgcttta cttcgacccg aagctgaccg
     2821 acaagctgga aggtctgtcg ttctccatgc gctttcggga taccccgctc agcgtgaatc
     2881 tcgaggccgg taagctgaag gtctcggtgg agcacggcgg cggtgggggc gctatcaaga
     2941 tcggcgtggg tgaggacgtg cagacgatag agcccggaga ggcccacgac ttcgacctgt
     3001 cgtcggcgac tgcaagcact acctgacgcc accgggatgg tgaagagttg accatgaagg
     3061 tggcgcccga gatcctgcgc agcaccgccc acgcggcggt ggatgcacgc ctcgagagcg
     3121 ggatgaccgt ggggctggga tccggcaccg cggcctccct ggccgttgag aggatcgggg
     3181 agttgctctc ctcgggcgag ctggaggatg tgcgggggat accaacctcc gagaagaccg
     3241 cgcggctggc ccgcgaggtc ggtatccccc tgctggacct ctccgcgtcc ggtcccgacg
     3301 tcaccataga cggcgccgac gagatcgggg agggaaacct ctcgttgatc aagggccacg
     3361 gcggcgccct gctgagggag aagatcgtcg catcctgcag ctacctggtc gtggtcgccg
     3421 atgcctcgaa gcttgtgaac gagctgggga ggggctcgat tccggtcgaa gtcgagcctt
     3481 tcggatggga ggcgacggtg gcggcgctct cctccctcgg ctgcgaggct accctcagaa
     3541 gggctcccaa caacccggag cttcccttcg tgaccgacgg tggccacttc accgtcgact
     3601 gcatgttcgg cccgatgccc gaccccgcat cgctagaagc ggagataagg cgcatacccg
     3661 gcgccctgga gtgcggcatc tttgtcgacc tggccagggt ggcgtacgtg gcccacgagg
     3721 caggtaccga catcatggaa gcgtgagtac ctcctgtgtt gtggtcgaga tacttaactg
     3781 acgacacggg agcgatttgc ggaaggagtg cccctgctgt gggtcgaggc aacaggtgaa
     3841 atccctgaga cctgaaacct cctcccgggt gggtcatcct tttcgagaac cgccatatta
     3901 cgggaggtag acatgggatt gttttcggat ctggatgaca agctggtcgg cggcccctct
     3961 attctcgcgg cagacttcgc caggctggcg gacgacgtca gcaaggccag agacggtggt
     4021 gcggccttta tacacttcga caccatggac aatcactacg tgccaaacct gtccttcggc
     4081 ccccccgtcg ccgcctcgct cgcctcgcat acggacttgc cgatcgacgt tcatcttatg
     4141 gtcgacaacc ccgacaacct gatccccgat tttctggatg cgggggtcac cagcatctcc
     4201 gtccacccgg aggcggtgac ccacctgcac cgcaccctcg ccgtgatccg ggacggcggc
     4261 gccgaagcgg gcgtggccct gaacccgacg acctcgcccg agaccctgga atggacgcta
     4321 ccttacctcg acttcattct ggtgctgagc gtcgacccgg gctttggagg gcaatcgttc
     4381 cttcccgagg cagcagaaaa gattcgtcgc ttgaa
//
LOCUS       DBNGVD010000620         3422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_1638814, whole genome shotgun sequence.
ACCESSION   DBNGVD010000620 DBNGVD010000000
VERSION     DBNGVD010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_1638814"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1437
                     /locus_tag="AC16CM_16450"
     CDS             121..1437
                     /locus_tag="AC16CM_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR3141475.1"
                     /translation="MIRSLLSYDRYWAYRSMLLVTVFAVAVGVWARFYNLGFPARILW
                     DEKYFPVMANRFLQGEYQFDLHPPLGKFIIALGISLFGNDPIGWRVMPLVFGLALLPL
                     VGALGWYCLRERVGALLLVIFFSVETIFIVNSRVGVMDIFLVFFVLATLLVALRAENG
                     RQVLLCSSLLGLAIATKWAAFPVALPVGYVLWRKGLFRQFVGGLWVSAIVYIGVVYVG
                     ALISVTANPVGAWLWVWDWHLKAADKISAAIPHGWGSPWWSWPIMLRPRRYFYGVDGD
                     GMLQVVMAIGNPLLWWSSTLAVLAGIVEVTRKTISNKLSVDDPIVPIVLGYVFLLLPW
                     LPGTRIPYLYNYLPIYPFALLALVYWLCKLWNRGPWGSWMVVGFTVAAVAMSVYFLPL
                     AIALPIEQSDLMNRIWTDYWFVFEKPVPGSGCLVPNPGELCPRPPD"
     gene            complement(1470..1928)
                     /locus_tag="AC16CM_16455"
     CDS             complement(1470..1928)
                     /locus_tag="AC16CM_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3141476.1"
                     /translation="MNGVVEGIFVTAEGSAAMERVEEVRTVEGCGIEGDRYCKGTGYW
                     TQYGDVCQVTLIEGEDLDYIQDELGIGVKEGQHRRNIITRGIRLLELRKKRFRVGDTL
                     LEFDRSRPPCKHVQEMSEPGMTRALKNRGGICARVIEAGSIKPGDTIETE"
     gene            complement(1980..>3422)
                     /locus_tag="AC16CM_16460"
     CDS             complement(1980..>3422)
                     /locus_tag="AC16CM_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3141477.1"
                     /translation="SNIKERRDCSTALFDARGLMVAQAEHMPVHLGAMPEAVAAIMGR
                     DPEPGDVFAINDPYSGGTHLPDITLVSPVSVEGEIVGYAVTRAHHSDVGGMRPGSMPS
                     DSREIYQEGIVIPPVRLVQGGEYVQDVLDLLLANVRTPDIRRGDLRAQIAANNIAEER
                     VRELMDRRGKETVITAFGEVVTYAEKRTREAIRDLPDGEHSAEDFMEGDGVTDEDIPI
                     RATVTVDGDSMTIDLTGTADAVAGNINCPLPVTRSACFFALRVLLPKDIPANAGTYVP
                     LEIETRKGSLVDATYPSAVVAGNVETSNRIADTVLEALSSFAPERIPAQGQGTMNNVI
                     IGDPGWTYYETIGGGQGAGAGGHGPSGVHIGMSNTLNTPIEALELEYPMRIERYELRY
                     DSGGAGDNRGGDGIVRSVRVLKPASLSLLTDRRRHPPQGMEGGEPGAVGRNLLGDEEL
                     PPKVSRELEEGDVISVETPGGGGYGHAGDD"
BASE COUNT          493 a         1141 c         1071 g          717 t
ORIGIN      
        1 gttggggtgt ttgcacacaa gccataattc cctttacccg ctcggggatg accgaaacgc
       61 cgacgggctc ctgtaccggc tcctttacaa acgcgcgcag aacctggaaa cttgcgcctt
      121 atgatcaggt cgcttctgag ctatgacaga tactgggcct accgctcgat gctgctcgtc
      181 acggtcttcg cggtggcggt cggggtgtgg gccaggttct acaacctcgg tttccccgcc
      241 aggattctgt gggacgaaaa gtacttcccc gttatggcca acaggttctt gcagggggag
      301 taccagttcg acctgcatcc gcccctcggc aagttcatca tagccctggg tatctccctc
      361 ttcggcaacg atcccatcgg gtggagggtc atgccccttg tcttcggcct cgccctgctg
      421 ccgctcgtcg gggcgctcgg gtggtactgc ctcagggaga gggttggggc actgttgctc
      481 gtcatcttct tctccgtgga gaccatcttc atcgtgaact ccagggtcgg ggtcatggac
      541 atcttcctcg tgttcttcgt gcttgccacc ctcctcgttg ccctcagggc cgagaacggc
      601 cgtcaggtgc tcctgtgttc ttcgcttcta gggctggcca tcgccaccaa atgggccgcc
      661 ttccccgtag ccttacccgt cggctacgta ttgtggagga aggggctctt ccggcagttc
      721 gtgggcgggc tctgggtctc cgccatcgta tacatcggtg tcgtgtacgt gggggccctg
      781 atctccgtca ccgccaaccc cgtcggggcg tggctgtggg tgtgggattg gcacctcaag
      841 gcggcggaca agatctccgc cgccatcccc cacggatggg gttcgccctg gtggagctgg
      901 ccgatcatgc tcaggcccag acgctacttc tacggggtgg acggggacgg catgctgcag
      961 gtggtaatgg ccatcggtaa cccgctgctg tggtggtcga gcacgctggc cgtgctggcc
     1021 ggcatcgtcg aggtaacgcg caagaccata tccaacaagc tctcggtcga cgacccgatc
     1081 gtgcctatag tcctcggcta cgtgttcctc ctgctgccgt ggctgcctgg caccaggatt
     1141 ccctacctct acaactacct cccgatctac cccttcgccc tgctggccct tgtctactgg
     1201 ctgtgcaagc tgtggaaccg tggcccgtgg ggctcctgga tggtcgtcgg ctttaccgtt
     1261 gcggccgtgg cgatgagcgt gtacttcctt cccctggcca tagccctacc catagaacag
     1321 tccgacctta tgaaccgcat atggaccgac tactggttcg tcttcgagaa accagtaccc
     1381 ggcagcggtt gcctggtccc gaaccccggc gagctctgtc cccgtccccc cgattgagag
     1441 acggtggttc gcgaaccgcc cctacaggct tattccgtct cgatggtgtc gccgggtttg
     1501 atgctgccgg cttcgatgac cctggcgcag atgccgccac ggttcttgag ggcgcgcgtc
     1561 atgcccggct cgctcatctc ctgcacgtgc ttgcagggcg ggcgcgagcg gtcgaactcc
     1621 aggagcgtgt cgcccacgcg gaaccgcttc ttgcgcagct ccagtagcct aatgccgcgc
     1681 gtgatgatgt tgcgccggtg ctggccctcc ttgacgccga tgcccagctc atcctggatg
     1741 tagtccagat cctcgccttc gatcagggtt acctggcaca cgtctccgta ctgcgtccag
     1801 taccccgtgc ccttgcagta gcggtccccc tctataccgc acccctcgac ggtcctgacc
     1861 tcctcgacgc gctccatcgc cgcgcttcct tctgcggtga caaagatgcc ctcgacgaca
     1921 ccgttcatgt tcgcgtagct ccctccacta gatggactcg gcccagaggt tagcagctac
     1981 tagtcatctc cggcgtgccc gtaacccccg ccgccaggcg tctcgaccga gatcacgtcc
     2041 ccctcctcca gctccctgct gaccttcggc ggcaactctt cgtcgccgag gaggttgcgg
     2101 ccgaccgcac ctggctctcc gccctccatg ccctgtggcg ggtggcggcg gcggtcggtg
     2161 aggagcgaga ggctcgcggg tttgagcacg cgcacggacc ttacgatacc gtcgccgccc
     2221 cggttgtcac cggcgccgcc cgagtcgtag cggagctcgt agcgttctat gcgcatgggg
     2281 tactctagct ccagcgcctc gataggggtg ttcagggtgt tgctcatgcc gatgtgcacg
     2341 ccggaggggc cgtggccgcc ggcgcctgcg ccctgaccac cgccgatggt ctcgtagtag
     2401 gtccagccgg ggtcgccgat gatcacgttg ttcatcgtgc cctgtccctg cgccggaatc
     2461 ctctccgggg cgaagctgga gagcgcctcg aggaccgtgt cagcgatcct gttgctcgtc
     2521 tccacgtttc cggctaccac ggcggatgga taggtggcat cgaccaggct gcccttcctg
     2581 gtctctatct ctagcggcac gtaggtcccc gcgttggcgg ggatgtcctt gggcaacagg
     2641 acgcgaaggg cgaaaaagca ggccgagcgg gtgaccggga gtggacagtt gatgttgccg
     2701 gccacggcgt cagccgttcc cgtgaggtct atcgtcatgg aatcgccatc cacggttacg
     2761 gtggcccgga tggggatgtc ctcatcggtt acgccgtcgc cctccatgaa gtcctcggcc
     2821 gagtgctcgc cgtcgggaag gtcccgaatc gcctcgcgag tacgcttttc ggcgtaggtg
     2881 accacctccc cgaaggccgt aatcacagtc tccttgccgc ggcggtccat cagctcgcgg
     2941 acgcgctctt cggcgatgtt gttggcggcg atctgggccc tcaggtctcc gcgccggatg
     3001 tccggcgtgc ggacgttcgc cagcaggagg tcgagcacgt cctgcacgta ctccccgcct
     3061 tgcaccagtc ggacgggcgg aatcacgatg ccctcctggt agatctcgcg cgagtcggag
     3121 ggcatggagc ctggccgcat cccgccgacg tcggagtggt gggcccgcgt aacggcgtag
     3181 ccgacgatct ctccttccac cgaaaccggc gagacgagcg tgatgtctgg caggtgggtg
     3241 ccgcccgagt acgggtcgtt gatggcgaac acgtcgcctg gctccgggtc gcgccccatg
     3301 atggctgcca cggcctcggg catcgcgccg aggtggaccg gcatgtgctc ggcctgggcg
     3361 accatcagcc cgcgggcatc gaagagcgcg gtggagcaat cgcgccgctc ctttatgttc
     3421 ga
//
LOCUS       DBNGVD010000621         7792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3641899, whole genome shotgun sequence.
ACCESSION   DBNGVD010000621 DBNGVD010000000
VERSION     DBNGVD010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3641899"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16CM_16465"
     CDS             complement(<1..868)
                     /locus_tag="AC16CM_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3141478.1"
                     /translation="MGDPRGFLKIGRKPTPKRTVVERVQDYRYVYEPMPNEELRGQAS
                     RCMDCGVPFCNTGCPLGNLIPDWNDLVYREHWKQAIDRLHQTNNFPEFTGMLCPAPCE
                     AACVLSINDKPVTIKEIELSIVNRAFEEGWVVAKPPPPEMRTGKRVAIVGSGPAGLAA
                     AQQLNWAGHTVVVFEKDDKIGGLLRYGIPDYKIEKWVVDRRIAIMEEEGIEFRTDARV
                     GENPTTEELRGEFDAVVLAVGALQGRDLDVEGRDLDGIHLAMDYLVQQNRRVAGLPVD
                     AEPISAAGKNVVI"
     gene            complement(868..5352)
                     /gene="gltB"
                     /locus_tag="AC16CM_16470"
     CDS             complement(868..5352)
                     /gene="gltB"
                     /locus_tag="AC16CM_16470"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR3141479.1"
                     /translation="MTPRVGLYDPTYEHDACGLGFVARLDGRVTRETVEEGLEVLHNL
                     EHRGTTGSDPETGDGAGILTQIPDGFFRRECGALGIDLPAAGGYGVGMLFEPGEAEGP
                     DCGDQLARICAEEGQELLGFRDVPVVPEAVGRVARSVMPRIRQFFVGRGSGDVAAFER
                     KLYVIRRRLHDAVEGSSGCYVVSLSSRTIIYKGLLKGRQLSRFYPDLGDGAFASAIAL
                     VHERFSTNTLGSWELAHPYRYIAHNGEINTIRGNINWMRARESRLRSGLFGEDLGKLS
                     PVIQKGQSDSAAFDNALELLHLAGRSLPHAVAMMIPEAWENDELMDPDRRAFYQYHSA
                     LMEPWDGPAAVAFTDGRVVGATLDRNGLRPARYSVTRDGRVIMASEDGALRVPAEDVV
                     ERWRLQPGKMLVVDTERRELLHDQDVKKPLFGRQPYREWLDEGEINLSELPEPRVDPR
                     PEPASLLQRQRAFGYTIEDERILLAPMAQNGKEPDGSMGTDTPLAVLSERPQLLFSYF
                     KQHFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETRKHCRRILIDQPVLTAKELEK
                     IRALADERATSTTLSTLFSVPTGERGMEEALGRLCEKAESAVKGGSAVLVLSDRGVGE
                     TQAAIPSLLATASVHHHLVRAGIRTATTLVVETAEAREVHHFALLVGYGATAVNPYLA
                     FETIEDLAGSHLLEGVDPQEAAKNYVKAVGKGLLKVISKMGISTLFSYSGAQIFEAVG
                     LNQELIDRSFTGTASRVGGVGMDALAREAIERHRNAFAAVEDNAGELEVGGEYQLRAQ
                     GQYHQWNQTSIPPLQRAVKTGSFETFQEFTRHFDEHSACFSTLRGLFGFEESPIPIEE
                     VEPAREIVKRFTTGAMSLGSISREAHETLAIAMNRIGGKSNTGEGGEDPGRFTPDENG
                     DNRRSAIKQVASGRFGVTTEYLVNADQLQIKMAQGSKPGEGGQLPGGKISEYIASIRY
                     STPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPYARVSVKLVAEVGVGTVAAGVAKA
                     KADHITISGHDGGTGASPLSSIKHAGLPWELGIAEAQQVLVENGLRGRVILQTDGHLK
                     TGRDVAVAALLGAEDFAFSTAPLVATGCIMMRVCHLNTCPVGIATQDPELRKRFTGTP
                     EHVVNYFFFVAEEVRQYMASMGFRTFEEMIGRTDRLRMREALEHWKAGGVDLSAILHR
                     AKAPEGEAIHHAEAQDHGLEKSLDNELIEKCLPALENGENVAFEHPILNTNRTVGGML
                     SGEVARRYGNEGLPDGTVRIGFHGVAGQSFGAWLARGLTFTLEGTTNDYVGKGLSGGR
                     LAVFPYRFAGYRASRNVVVGNVALYGATAGEAYFRGFAGERFAVRNSGAHAVVDGVGD
                     HGCEYMTGGVVVVLGETGRNFAAGMSGGVAFVLDPDGRFDALCNKDMVGLEPVEDEED
                     VELLRGMVERHLEWTGSEQAQRVLERWEEMLPRFVKVMPHDLKRVLQERQEAELEVAT
                     "
     gene            complement(5584..5910)
                     /locus_tag="AC16CM_16475"
     CDS             complement(5584..5910)
                     /locus_tag="AC16CM_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141480.1"
                     /translation="MANRFFKETSAEIISDLLSDARVKRVREYVGGEEFKADRVRVRQ
                     DAAKRLKNIRERYRTKRRDPETAARERELTLRLAEVEAEAADLRIQLSELLAEEEKLR
                     TALDEL"
     gene            complement(5959..6534)
                     /locus_tag="AC16CM_16480"
     CDS             complement(5959..6534)
                     /locus_tag="AC16CM_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HIR3141481.1"
                     /translation="MNDKGRTGSGDEDHAVLAGQADGEGGEPDLQLDVPSLSVEEIEL
                     EVEDLQVHLSIQAELADLVKINIGLDAVLGEAKLGIKGVEAQVQLKARLDNVRDIFSE
                     VLASLQHSPQFFRQPSETATTAEADNVLSPPAREETGTPQDVAGDLVEPEATEAAVRR
                     ARELGVDLTNLRGTGAGGRIVVRDVTRAAEE"
     gene            6701..6916
                     /locus_tag="AC16CM_16485"
     CDS             6701..6916
                     /locus_tag="AC16CM_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141482.1"
                     /translation="MSQQDQQGQQQEGQQQSPLQLLGRALTDIRKVQNLLELKYPDQG
                     EAIEMQREAGDLVWDEIQRMQQQQQQK"
     gene            complement(7010..7297)
                     /locus_tag="AC16CM_16490"
     CDS             complement(7010..7297)
                     /locus_tag="AC16CM_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141483.1"
                     /translation="MYEARNAVGPTPSEDRRMWRLLLARLHPDAGGDHDLFAFACAVR
                     DEVHREERTAGSAAGTENGTRTASDVAPFLGTWHEAMGRWSTSNREALGKI"
     gene            complement(7474..>7792)
                     /locus_tag="AC16CM_16495"
     CDS             complement(7474..>7792)
                     /locus_tag="AC16CM_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141484.1"
                     /translation="DTAGQATGQAGQMVDQAQDTAGGLVGWQGADQGTGQGSGGGSGQ
                     PGEPEMDDYSNSDSNSETSVNEKSPKGILGQLLNLDILPPPQSDTTTFESGEGNEFYG
                     GQQ"
BASE COUNT         1211 a         2627 c         2489 g         1465 t
ORIGIN      
        1 ggatcacgac gttcttgccg gcggcgctta tgggctcggc gtcgacgggc agtccggcca
       61 ccctcctgtt ctgctgcacg aggtaatcca tcgcgaggtg gatgccgtcg agatcccgcc
      121 cctccacgtc gaggtcgcgc ccctgcaagg cgccgacggc gaggaccacg gcgtcgaact
      181 cgcctctcag ctcctccgtc gtggggttct cgccgacacg ggcgtccgtc cggaactcga
      241 tgccctcctc ttccatgatc gcgatacgcc tgtccacgac ccacttctcg atcttgtagt
      301 cgggaattcc gtagcggagt aggcccccga tcttgtcgtc tttctcgaaa acgacgaccg
      361 tgtgcccggc ccagttcagc tgttgcgccg ccgcgagccc ggccggaccg ctccccacga
      421 tggcgaccct cttacccgtg cgcatctcgg gcgggggggg cttcgccacc acccatccct
      481 cctcgaaggc gcggttgacg atcgagagct ctatctcctt gatggtgacg ggtttgtcgt
      541 ttatggagag aacgcaggcg gcctcgcagg gggcgggaca gagcatgccc gtgaactcgg
      601 ggaagttgtt ggtctggtgc aggcggtcga tggcctgctt ccagtgctcc cggtagacga
      661 ggtcgttcca gtcggggatg aggttcccga gcgggcagcc ggtgttgcag aagggcacgc
      721 cgcagtccat gcagcgcgag gcctggccgc gtagctcctc gttgggcatc ggctcgtaga
      781 cgtaacggta gtcctggaca cgctccacga cggtccgctt cggggtgggc ttgcggccta
      841 tcttcaggaa acccctggga tcccccatca ggtcgccacc tccagctccg cctcctggcg
      901 ctcctgcaac acccgcttga gatcgtgcgg catcaccttc acgaagcggg gcagcatctc
      961 ctcccatcgt tcgagcacac gctgcgcctg ctcgctcccc gtccactcca ggtgccgctc
     1021 gaccatgccg cgcagcagct cgacatcctc ttcatcctcc acgggctcca gcccgaccat
     1081 gtccttgttg cacagggcgt cgaagcgtcc gtccgggtcg agcacgaagg cgacgccgcc
     1141 gctcatgccg gcggcgaagt tgcgcccggt ctcgccgagc accaccacga ccccgccggt
     1201 catgtactcg cagccgtggt cgccaacgcc gtcgaccacg gcgtgtgccc ccgagttcct
     1261 gacggcgaac ctttcccccg cgaagcccct gaagtacgcc tcgcccgccg tcgcgccgta
     1321 gagggcgacg ttgccaacca ccacgttcct ggacgccctg tagcccgcga accggtaggg
     1381 gaacacggcg aggcggcctc cggagaggcc cttgccgacg tagtcgttgg tcgtgccctc
     1441 cagggtgaag gtcaggccgc gcgcgagcca cgccccgaag gactgccccg cgacgccgtg
     1501 gaaacctatg cggaccgtgc cgtccggcaa gccctcgttg ccgtagcgcc gcgcgacctc
     1561 gccggagagc atcccgccga ccgtgcggtt ggtgttcagt atgggatgct cgaaggcaac
     1621 gttttcaccg ttttcgaggg cgggcaggca cttctcgatc aactcgttgt ccagggactt
     1681 ctcgaggcca tggtcctggg cctcggcgtg gtgtatggcc tccccttccg gggccttggc
     1741 ccggtggagg atcgcggaga ggtccacgcc cccggccttc cagtgctcga gggcctcgcg
     1801 catcctcagc ctgtcggtgc ggccgatcat ctcctcgaag gtcctgaagc ccatgcttgc
     1861 catgtactgc cgcacctctt cggcgacgaa gaagaagtag ttgacgacgt gctccggcgt
     1921 accggtgaaa cgcttcctga gctcggggtc ctgcgtggcg atccccaccg ggcaggtgtt
     1981 gaggtggcag acgcgcatca tgatgcagcc cgtcgccacg agcggcgcgg tcgagaacgc
     2041 gaagtcctcg gccccgagca gcgcggccac ggcaacgtcc cggcccgtct tgagatggcc
     2101 gtcggtctgc aggatgacgc gcccccgcag gccgttctcc accagcacct gctgggcctc
     2161 ggcgatgccc agctcccacg gcagcccggc gtgctttatg gaggagagcg gcgacgcacc
     2221 cgtgccaccg tcgtggccgg agatggtgat gtggtcggcc ttcgccttcg cgaccccggc
     2281 ggcgaccgtc ccgaccccca cttcggccac gagcttcacg ctcaccctgg cgtacgggtt
     2341 ggcgttcttc aggtcgtgga tgagctgggc gaggtcctct atggagtaga tgtcgtggtg
     2401 gggtgggggg gagatcaggc cgaccccggg cgtggagtag cggatgctgg ctatgtactc
     2461 gctgatcttg ccccctggca actgcccgcc ctcgccgggc ttggagccct gggccatctt
     2521 gatctgcaac tggtcggcgt ttacgaggta ctcggtggta acgccgaaac gcccgctggc
     2581 gacctgcttt atggcgctgc ggcggttgtc gccgttctcg tctggcgtga agcgcccggg
     2641 gtcctcaccg ccttccccgg tgttggactt gccgcctatg cggttcatgg ctatggcgag
     2701 ggtctcgtgc gcctccctgg agatggaccc gagggacatg gcccccgtgg tgaagcgctt
     2761 gactatctcc ctggcaggct ctacctcttc tatagggatg gggctctcct cgaacccgaa
     2821 gaggccgcgc agcgtagaga agcaggcgct gtgttcgtcg aagtgtcggg tgaactcctg
     2881 gaaggtctcg aagctccccg tcttgacggc ccgctgcagg ggagggatgc tcgtctggtt
     2941 ccactggtgg tactgaccct gcgccctgag ctggtactcg cccccgacct cgagctcccc
     3001 ggcgttgtcc tcgaccgccg cgaaggcgtt cctgtgacgc tcgatggcct cccgcgccaa
     3061 cgcgtccatc ccgacgccgc caacccgcga ggcggtgccc gtgaaggacc ggtctatgag
     3121 ctcctggttc aggccaacgg cctcgaagat ctgggcccca gagtacgaga agagcgtgga
     3181 gatgcccatc ttggagatca ccttcagcag gcccttgcca acggccttga cgtagttttt
     3241 cgccgcctcc tgggggtcga cgccttccag caggtgcgag ccggcaaggt cttctatggt
     3301 ctcgaaggcg aggtaggggt tcacggcggt ggcgccgtag ccgaccagga gggcgaagtg
     3361 gtggacctcc cgcgcctcgg cggtctccac aacgagcgtg gtcgccgtcc ttatgccggc
     3421 ccgcaccagg tggtggtgga cgcttgccgt ggccagcagg ctgggtatcg cggcctgcgt
     3481 ctcgccaacg cccctgtcgc ttagcacgag cacggccgag ccgcccttta ccgcgctctc
     3541 ggccttctcg cacaaccggc caagcgcctc ctccatcccc ctctcgccgg tggggacgga
     3601 gaagagggtg gagagggtcg tggaggtggc gcgctcatcg gcgagggccc ggatcttctc
     3661 cagctccttc gccgtcagga ctggctggtc tatgaggatc ctgcggcagt gcttcctggt
     3721 ctcgtcgaag aggttctgct ccgggccgag gctcatcttg agcgacatga cgagctcctc
     3781 gcgcaacggg tctataggag ggttggtgac ctgcgcgaag tgctgcttga agtacgagaa
     3841 gaggagctgc ggccgctcgg agagcaccgc cagcggtgtg tccgtgccca tcgagccgtc
     3901 gggctccttg ccgttctggg ccatcggggc gaggagaatg cgctcgtcct cgatggtgta
     3961 gccgaaggcc cgctggcgct gcaggaggct ggcaggctcg ggacgcgggt cgacgcgggg
     4021 ctcggggagc tcgctcaggt tgatctcccc ctcatccagc cactcgcggt agggctgccg
     4081 cccgaacaaa ggcttcttga cgtcctggtc gtgcaacagc tcgcgacgct ctgtgtcgac
     4141 tacgagcatc ttgccaggct gcagcctcca gcgttcgacc acgtcttccg caggcaccct
     4201 gagggccccg tcttcggagg ccatgatgac ccggccgtcc ctggtgacgg agtagcgggc
     4261 cggacgcaac ccgttcctgt ccagcgtcgc gccgacgacg cggccgtcgg tgaaggcgac
     4321 cgcggccggt ccatcccacg gctccatcag ggcgctgtgg tactggtaga aggcacgacg
     4381 gtcagggtcc atcaactcgt cgttctccca ggcctcgggg atcatcatgg ccaccgcgtg
     4441 cggcaggctc ctccccgcga ggtgcaagag ctcaagggcg ttgtcgaagg ccgccgaatc
     4501 gctctggccc ttctggatga cgggcgagag cttgccgagg tcctccccga agagccccga
     4561 tctaagacgg ctctcccggg cgcgcatcca gttgatgttg ccccttatgg tgtttatctc
     4621 accgttgtgc gcgatgtacc ggtacggatg ggcgagctcc caactcccaa gcgtgttggt
     4681 cgagaaccgc tcgtgtacga gcgcgatggc gctggcgaag gccccgtccc cgagatccgg
     4741 atagaaccgc gacaactgcc ggcccttgag aagccccttg tagatgatgg tcctcgacga
     4801 gaggctcacc acgtaacaac cgctggagcc ctcgacggcg tcgtgcagcc ggcgcctgat
     4861 gacgtaaagc ttccgctcga aagccgccac gtccccgctc ccacgaccta cgaagaactg
     4921 cctgatgcgc ggcatgacgc tgcgcgccac ccggcccacg gcctcgggca cgacgggcac
     4981 gtccctgaaa ccgagaagct cctggccctc ctcggcgcag atccgcgcga gctggtcccc
     5041 acagtcgggc ccttcggcct cccccggctc gaagagcatc cccacaccgt agcccccagc
     5101 cgccgggagg tctatgccga gggccccgca ctccctgcgg aagaagccgt ccgggatctg
     5161 ggtcaaaata ccggccccgt caccggtctc ggggtcgctg cccgtcgtcc ccctgtgctc
     5221 caggttgtgc aggacctcaa gaccctcctc gacggtctcg cgcgtcacgc gcccgtcgag
     5281 cctcgctacg aacccgagcc cgcaagcatc gtgctcgtac gtggggtcgt aaagtccgac
     5341 tctaggggtc aacaagcggt tactccttgg tgtcgagggc acggagcgac tatttttaca
     5401 cgaatcgcca gcagcgcaca agtaggtaac aagcttgttg catactagta ttttgcaagg
     5461 attctggaac gtcgcaaggc atcgtaacgc tttgcgagct atcagccatt agctatcagc
     5521 catcagcaag aacaagaagc tgatcgctga cggcttgcat gacactccgg tgctggagaa
     5581 acgctagagt tcgtccaggg cggtacggag cttctcctcc tctgcgagca actcggagag
     5641 ctgaatgcgg aggtcggcgg cctcggcctc tacctcggcg aggcgaagcg ttagttccct
     5701 ctcgcgggcc gcggtttcgg ggtcgcgtcg tttcgtgcgg tagcgctcgc ggatgttctt
     5761 taggcgtttt gcggcgtcct ggcgtacccg cacgcggtcg gccttgaact cctcacctcc
     5821 cacgtactcc ctcacgcgtt tcacgcgtgc gtcggagagt aggtccgaga ttatctccgc
     5881 cgatgtctct ttgaagaacc tgttggccat gggtctcctt ccgctccccc tgattgtata
     5941 cgctgcaggg ggtcgaggtt attcctccgc ggcccgcgtg acatccctga cgactatgcg
     6001 gccgcccgca cccgtgcccc gaaggttagt caggtccacg ccgagctccc tcgccctcct
     6061 cacggccgcc tccgtcgctt ccggttccac gaggtcccca gccacatctt gcggggttcc
     6121 cgtctcctct cgcgccgggg ggctcaggac gttgtcggcc tctgcagtcg tcgcggtctc
     6181 ggacggctgg cggaagaact gcgggctgtg ttgtagggat gcaagcacct cgctgaagat
     6241 gtcgcggacg ttgtcgagac gggctttcag ctgcacctgg gcttccacgc ccttgatccc
     6301 tagcttcgcc tctcccagca cggcgtcgag cccgatgttt atcttgacca ggtcggccag
     6361 ctcggcctgg atggagaggt ggacttgcag gtcctcgacc tcgagctcga tctcctcgac
     6421 gctgagagag ggcacgtcga gctgcaggtc gggctcccca ccctcgccat ctgcctgccc
     6481 ggcgagtacg gcgtggtctt cgtcgccgct gcctgtacgc cccttatcgt tcaatgcccg
     6541 aacctccgat gccatgcacc ccgctccagc gtctcgttac acatctgtca tgcccccact
     6601 ttacggcaaa taaactcgcc ggggaacgcc gcgctgtctc gggccggccc gtaacgggta
     6661 taaaccggag cacgagcagc aagagtaaag tgggggcccg gtgtcacagc aagatcagca
     6721 aggccaacag caagaaggtc agcaacagag cccgctgcag ttgctgggac gcgcgttgac
     6781 cgacatcagg aaggttcaga accttctaga gctcaagtat ccggaccagg gcgaggcgat
     6841 agagatgcag cgcgaagccg gggatctggt ctgggacgag atccagcgca tgcaacagca
     6901 gcaacagcag aaataacgaa acccgatcgg cgcttgaagg cagcaggata caggaagatt
     6961 gcctgatgcc tgtagccttt cctcaggttt actctttgcg caaatcgcct tagatcttgc
     7021 cgagggcttc gcggttgctg gtggaccacc ggcccatggc ctcgtgccag gtgcccagga
     7081 agggcgccac atccgacgcg gtcctggttc cgttctcggt tcccgccgcg cttccggcag
     7141 tacgttcttc ccggtgaacc tcgtccctca cggcgcaggc gaacgcgaag aggtcgtggt
     7201 cgccgccggc atcggggtgc aggcgtgcca acagcaatcg ccacatacgc cggtcttcgc
     7261 ttggtgtggg tcccaccgcg tttctggcct cgtacactag atcgacctcc gttcgggggc
     7321 attctatgcg gtgggacgaa atcctgtaaa ccacgatatc gggcaaccgc tacacctgga
     7381 ggtgcagcgg tttgccgggt ctacatatgt gccgggccga ccacggggag cgggagccgt
     7441 cggtctaccc ccaggctggc tcgtgcggag tgcttattgc tgtcccccgt agaactcgtt
     7501 gccttcaccg gactcgaagg tggtagtgtc gctctgcggg ggagggagga tatccaggtt
     7561 gaggagttgc ccgagaattc ccttcggtga cttctcgttg acggacgtct ccgagttgct
     7621 gtcgctgttg gaataatcgt ccatctccgg ctcaccgggc tggccggagc ctccaccgga
     7681 gccctgcccg gtgccctggt cggcaccctg ccagccgacg agcccgcccg cagtgtcctg
     7741 ggcctggtcc accatttggc ccgcctgacc cgtggcctgg cctgcggtgt cc
//
LOCUS       DBNGVD010000622         3338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_909600, whole genome shotgun sequence.
ACCESSION   DBNGVD010000622 DBNGVD010000000
VERSION     DBNGVD010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_909600"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16CM_16500"
     CDS             <1..509
                     /locus_tag="AC16CM_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3141485.1"
                     /translation="HYAEAVGRGADPETVLAALRIRSRDNARTPVQWDDTENAGFTTG
                     TPWISVNPNHEVVNARAEIADEDSVFHHYRRLIELRHTEPAVAHGDFNMLLADHDQVY
                     AFTRRYGSTGLLVLANFFGDPVPIEVPDASLWQDAELLLGNYPVDAADNLRDLTLRPF
                     EARIYRSR"
     gene            714..935
                     /locus_tag="AC16CM_16505"
     CDS             714..935
                     /locus_tag="AC16CM_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141486.1"
                     /translation="MNEVVYAIWDIAWEEQWFLYELGEADDGYHCRCVDAYSWETVVL
                     GLGDSPTEALARALGELLAHAGREPPGDR"
     gene            1129..1617
                     /locus_tag="AC16CM_16510"
     CDS             1129..1617
                     /locus_tag="AC16CM_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141487.1"
                     /translation="MYGRGMMGYAGGRRAMVPHGRGFHPRFPLGLVVGGLFLLFFVGH
                     AVHFFPFLLILAVPFLLVPVLAAAGRGLAGVAEARTRERTDRATSEESKERELLRALA
                     RRGELSPALAALETSLTVAEADRMLSGLAESGHLEVRAHEGRIGYSLWEQDRRQLTDG
                     ER"
     gene            1646..2182
                     /locus_tag="AC16CM_16515"
     CDS             1646..2182
                     /locus_tag="AC16CM_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3141488.1"
                     /translation="MFGPKAMKGFFGGHGPGFGHGPRRGRMFEKGDLKYVILDMLQKE
                     PAHGYEIIRRLEERSRGFYSPSPGSVYPALQLFEDMGYVTYAQHDGKKVYSITDEGRA
                     YLEENRRSIDAIWGRVGGGWNPQAAAEMHEIRHDLMSLGKLFGQHMHEGKIDQKKMAR
                     IREVISGATRQIEDILRG"
     gene            2535..>3338
                     /locus_tag="AC16CM_16520"
     CDS             2535..>3338
                     /locus_tag="AC16CM_16520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3141489.1"
                     /translation="MVRGRISGKTRLLALIGHPVGHSLSPAMHNAAFEEDGLDFVYVC
                     LDVDPEDLPGAVGGMEALKFRGFNVTMPHKRAMVGLVDGLDDGARVSGAVNTVVIEAS
                     GTRGYNTDGGGMVMACEEAGIGLSGESVLLLGSGGTAAAIAVAFGEAGVGNLHIASRD
                     VENAARLREELRPTGMEKLSVGSLAGLERDAPDAGVIVNATPLGMKDGDLLPLPPEHV
                     QGAGAICDVVYRPGTETLLVRLAKEGGARVVAGGRMLLYQGVLAQKLWTG"
BASE COUNT          611 a         1027 c         1140 g          560 t
ORIGIN      
        1 accactacgc cgaggccgtc ggcaggggtg ccgatcccga aaccgtcctg gccgcgcttc
       61 gcatccgcag ccgcgacaac gcccgcaccc cggtgcaatg ggacgacacc gagaatgccg
      121 gcttcacaac cggcacacca tggatctcgg tcaaccccaa ccacgaggta gtcaacgctc
      181 gggccgagat cgccgacgaa gactcggtgt ttcaccacta ccggcgcctg atcgaactgc
      241 gtcacaccga gcccgcggtt gcgcacggcg acttcaacat gctgctcgcc gaccacgacc
      301 aggtctacgc gtttacccgt cgttacggca gcaccgggct tctcgtactc gccaacttct
      361 tcggagaccc cgtccccatc gaagtcccgg acgccagcct ttggcaggat gccgagctcc
      421 tcctcggcaa ctacccggtc gatgccgccg ataaccttcg agacctgacg ctgaggccct
      481 tcgaagcgcg catctaccgt tcgcgctagc tcgatgcacc ggcaggatcg cgaaacctcg
      541 gtccaatacc cgtagaacgg tggaaatggc ctgaagtgcg catggcgctc ggcccgtgtc
      601 gtgcgaccat agggtgtgat cggcgcgcaa cccgacgtgg gtttgaagga ggcgacctca
      661 agtctaccag gaactctcct gccaagggat cgagagctgg acgggttccc gagttgaacg
      721 aggtcgtcta cgctatctgg gacattgcgt gggaggagca gtggtttctc tacgaactag
      781 gagaggccga tgacggctac cactgccgct gcgtggatgc ctactcctgg gagaccgtgg
      841 ttctcggtct gggagattcg ccaacggagg ccctcgcccg tgctcttggt gaactactgg
      901 cccatgccgg gagggagcca cccggagacc gataaccgca tggtcgaacg gtatgtacgc
      961 ccccgggcgt gctcgacccg gatcttctcc tccccggcgg ggtagttcga gcgggcaact
     1021 atcgagtccc catgccccga ggttcccgat cccggttcta ggcagtatat cacgatatat
     1081 cgtgatatac tgccacgtta caagggtcgt ccggacagag gaggcgagat gtacggtcgg
     1141 ggcatgatgg gctacgctgg tgggaggcgg gcgatggtgc ctcacgggag gggattccat
     1201 ccgaggtttc cgttggggtt ggtggtggga gggttattcc tgctcttctt cgttgggcat
     1261 gccgttcact tctttccgtt cttactgatc ctggccgttc ccttcttgct ggtgccggtt
     1321 ctggcggcgg ccgggcgggg gttggcggga gttgccgagg cccggacgcg ggagaggacg
     1381 gacagggcga cctcagagga gagcaaggag cgggagctcc tcagggcgct cgcgcgacgg
     1441 ggagagctct cgccggctct ggctgcgttg gagacctcgc tcacggtggc cgaggcagac
     1501 aggatgctct ccggccttgc cgagagcggc catctggagg taagagcgca cgaaggcagg
     1561 atcgggtact cgctctggga gcaagacagg cgccagctga cagacgggga gcgatagaca
     1621 gcgttccgca gaggagaccg aagacatgtt cggaccaaaa gcgatgaagg gtttcttcgg
     1681 cgggcacggg ccggggttcg gccacggccc gcggcggggt cggatgttcg agaagggtga
     1741 cctcaagtac gtcatcctcg acatgctcca gaaggagcca gcccacggct acgagatcat
     1801 aaggaggcta gaagagcgtt cccgcggctt ctactcacca agccccggtt ccgtctaccc
     1861 cgcgctacag ttgttcgagg acatgggcta cgtcacgtat gcccagcacg acggcaagaa
     1921 ggtctactcc atcacagacg aaggccgggc ctacctcgaa gagaaccggc gctccatcga
     1981 cgccatctgg ggccgggtcg ggggaggctg gaacccccag gccgccgccg agatgcatga
     2041 gatccgtcac gacctcatgt ccctgggcaa gctcttcggc caacacatgc acgagggaaa
     2101 gatagaccag aaaaaaatgg cccgcattcg cgaagtcatc tcaggcgcca cccgccagat
     2161 agaggacatc ctcagaggct aggcaacgag ccgcaagcgg ctcgttgccg cacggcgcta
     2221 cgcgcctttt cggcacgctc cagcctagcg gctgtcgctt ttcggcaacg cttcggcttc
     2281 gcctcgcttt cagccggtcg tctctctccg taacagcagc ttcgactacc cacctttccg
     2341 ggcggcgaat cgtcagagcg gcagggtgca ggccgtagcg ggatctagct gacagcgagc
     2401 cctgtaaggg cgaagcgttg ctgacaagcg agccaggcga agccgggcga gcgtgctgac
     2461 gagccggcgc agcggagcga agccggcgtg ctgacgagcg gaggccgaag gccgcagcgt
     2521 gctaacatgc catcgtggtg agagggagga tcagcggcaa gacgcggctt ctggccctga
     2581 tcgggcaccc cgttggtcat agcctgagcc cggcgatgca caatgccgcc ttcgaggagg
     2641 acgggcttga tttcgtctac gtctgcctgg acgtggaccc cgaagatcta cccggggcgg
     2701 tgggcggtat ggaggccctc aagttccggg gtttcaacgt cacgatgccg cacaagcggg
     2761 cgatggtcgg gcttgtcgat gggttggacg atggggcccg cgtctccggg gcggtcaaca
     2821 ccgtggtgat cgaggcttca gggacgcgcg gatacaacac ggacgggggc gggatggtga
     2881 tggcctgtga ggaggcgggc atcgggctct ccggagagag cgttctgctt ctcggctcgg
     2941 gcggcaccgc cgcggccatc gccgtcgcct tcggtgaggc gggggtcggc aacctgcaca
     3001 tcgccagcag ggacgtcgag aacgccgcac gcctccggga ggagctgcgc ccgaccggca
     3061 tggaaaagct ctcggtaggc tccctcgcgg gtctggaacg agacgcgcca gacgcgggcg
     3121 tgatcgtcaa cgcgacccca ctcgggatga aagacggtga cctcttgccc cttccacccg
     3181 agcacgtgca gggagccggg gcgatctgcg acgtggtcta ccgtccgggg acagagacgc
     3241 tgctggtcag gctggcgaaa gaggggggcg cccgcgtcgt cgcgggaggc cgtatgttgc
     3301 tctaccaggg ggtcctggcg cagaagctgt ggaccggg
//
LOCUS       DBNGVD010000623         7299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3642115, whole genome shotgun sequence.
ACCESSION   DBNGVD010000623 DBNGVD010000000
VERSION     DBNGVD010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3642115"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1258)
                     /locus_tag="AC16CM_16525"
     CDS             complement(233..1258)
                     /locus_tag="AC16CM_16525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3141490.1"
                     /translation="MRFSVVLGKLLFNRVLYVSVFLLVVLFASLGCNAADPQTNTDSQ
                     AKKIATFDGGSVTQGELDQFAEQSGFGDLSQDDPQYKAAIQQIMPQLVGIEIAKAYAS
                     EQNITVSDKEVDQEIEKIKKQVGEQARSSGQDLSNQKAYEQALKQNNITEEQLRDDIR
                     ENLPVQKVQEQVSNDAEPSDEEIQEFYDQNKESQFTTPEQVCVSHILFGKDQKQKAED
                     VKKQLEDGDDFAKLAEQYSQDPGSAEKGGDLGCLGKGETVPPFEKAAFGAEEGEIVGP
                     VKTQFGYHLLEVTDKKPQQVRPLDEVESQIRAQLASQKQSEAFSKWLEEQKKQRDVKY
                     LEGHKPA"
     gene            complement(1399..4563)
                     /gene="mfd"
                     /locus_tag="AC16CM_16530"
     CDS             complement(1399..4563)
                     /gene="mfd"
                     /locus_tag="AC16CM_16530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3141491.1"
                     /translation="MSERTQSRLLRAPRRVRAYLAATADRPVLLLAAGEEAAERYARE
                     AAVFTDGLVVHLPSRGVFYGDVFEPAVTRVGRRQRSLRSLRSAHVVVAGPLALMERTP
                     LYEPLYLAAETEIELDATLERLVALGYERVDRASRPGEFAVRGGIVDIFPSTRRSPIR
                     VEWWGDEVESVRAVSLATQRVVRELEAVTVFAAREGDLAALAATDDGLPEEARRGVRV
                     PGLDRLLLGLDPVSPRGLLPEGVEVWREEPQEDLPEEIEGFVPDLYDTEPPAPDVEFS
                     LGEEGEAVSAPPVVAFSDTLREAARRLDTLVEDGLAVFVACSSVGEARRTIYAFGEIN
                     RQMREAERVDATLPPGLYAVPGEVEEGFTYPDGGIAVFCRDSLLGRRRESRRRSGGRS
                     LGSFADLKVGDLVVHEMQGIGRFEGLVSQTVLGATRDYMRVGYRGGDTLFVPYEQMEL
                     LHKYVGGEGARLDKLGGATWTQVTDRVRKRVKALAGELLRLHAQRATAPGIAFPEDGE
                     WELELEESFPYQETPDQEAAITAVKTDMKRPHPMDRLVCGDVGFGKTEVSVRAAFKAA
                     LAGKQTMMLAPTTILVQQHERTFRQRLGAYAVRVESLSRFTPQAQRRRILADFAAGEV
                     DVLVGTHSLLGSQIAPKDLGLVVVDEEQRFGVKHKERIKEYKASVDLLTLTATPIPRT
                     MQMGLASLRDISVIETPPAGRRSILTHVGPYDEDLVKNAIEREVARGGQTFFVHNRVE
                     SIDAVTERLRELVPGARFVTAHGQMPERALEGVMQHFLDGQADVLVTTTIVESGLDVA
                     TANTLIVDRSDAMGLAQLYQLRGRIGRSRQQAYAYLFAPVGATVESQKRLEALLDFTE
                     LGSGFAIAMRDLEIRGAGNLLGAEQSGHIAAVGFEMYLNLLEEAVAAAKGEPQERAEE
                     RPVIVELTLDAYLPPGYVMDEIERVDLYRRASGLRSLAEVEDLAEELVDRFGELPEPS
                     RNLLGLSRVKILARESGASSVTFRSGNLTVTGATAGSAAPALIRRATGGTVTTRTGRI
                     SVRADEQDPLTLAEETLRAVIRSR"
     gene            4673..5086
                     /locus_tag="AC16CM_16535"
     CDS             4673..5086
                     /locus_tag="AC16CM_16535"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3141492.1"
                     /translation="MTVLDELNERGAGTLPGLIGLEIKGAEEGVISSRLALRDELMAP
                     NGYLHAATVVALADTSCGYGTFVNLPDGAESFTTIELKSNFVGTKRGGAISCRARLVH
                     GGRTTQVWDAEVSDEESGKPIALFRCTQMILYPRG"
     gene            complement(5099..6001)
                     /locus_tag="AC16CM_16540"
     CDS             complement(5099..6001)
                     /locus_tag="AC16CM_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3141493.1"
                     /translation="MRPRDFAGLIVLGALWGGAFLFIRVVVPALGPFLLVGLRVGLAA
                     AVLALYALAVGRVPKIRSRWRAFLVLGLFNSAVPFSLISAAEIHLTASLAAIINSTTV
                     MFTAMVGAVWMGEALTAKKIFGILLGIFGVAVLLGWDPLPLNGAVLLSVVAMLLASLS
                     YALGATYAKHYFSGIPPTGMAIGQLTAATVFLLPLSAASVPEQAPSLVVVLCMLGLAL
                     LSTALAYLIYFRLIENVGPTSTVTVTLLVPVFGLLFGVLLLGEPFGSGTIAGLALILC
                     SVVLITGIKPSAVSRQQPGRKADR"
     gene            complement(6096..6614)
                     /locus_tag="AC16CM_16545"
     CDS             complement(6096..6614)
                     /locus_tag="AC16CM_16545"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3141494.1"
                     /translation="MKAYVDEVASPAGPLVFAVDEDGALIRLHFVESNYGPVMEEDLE
                     DEGYTVRSDPTKTAPVRAELAEYSSGERRAFDVPLAFSGSEWQKAVWAELTRIPFGET
                     RSYGEVADALGRPGAARAVGSANARNVLPVVVPCHRVIAADGTLGGFNGGLHLKERLL
                     EHETRVLAATER"
     gene            complement(6611..>7299)
                     /locus_tag="AC16CM_16550"
     CDS             complement(6611..>7299)
                     /locus_tag="AC16CM_16550"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3141495.1"
                     /translation="TAVQRCRRLLDLDADPAAVTDVLGDDPMLGPIVRRSPGRRVPGS
                     VDGAELAFRAVLGQQVSVAGARTLAGRLVARCGAPLPASLLSENGLTHIFPGPEDVAG
                     ADLHRFGVPAARAEALRTLARALDGDVSLDPGSDRDEVERRLLGLRGIGPWTASYVAM
                     RALGDPDAFLPTDLGVRQAVRKLGHPDDRRSVATLAETWRPWRAYATQHLWASLAEEP
                     DRNENREVVA"
BASE COUNT         1248 a         2451 c         2363 g         1237 t
ORIGIN      
        1 tagcttctag ctatcagctt tcagcagcca gctgaccagt aattaacggt gtgcccgaac
       61 acccacgttc cccaaacctg tgcagcgagc ccttttcact agggtcctgt tggcttctcc
      121 ccgaagacgt caacttccgt agctgacggc tgatggctga ctgctctgcg ctaactctac
      181 gcgctgccga tccgccgcgc caccggtcta cctgaagcct taaacctgat gcctacgcgg
      241 gcttgtgtcc ttcgaggtac ttcacgtcac gctgcttctt ctgttcctcc agccacttgc
      301 tgaaggcttc tgactgcttc tgggaagcga gctgggccct tatctgcgac tcgacctcgt
      361 ccaggggacg gacctgctgg ggtttcttgt cggtgacctc cagcagatgg tagccgaact
      421 gggtcttgac ggggccgacg atctcgcctt cttcggcgcc gaaggccgcc ttctcgaagg
      481 gcggaacggt ctcgcccttg ccgaggcagc cgagatcgcc acccttctcc gcgctaccgg
      541 ggtcctggga gtactgctcg gcgagcttgg cgaagtcatc gccgtcttcg agctgcttct
      601 tcacgtcctc ggccttctgc ttctggtcct tgccgaagag gatgtggctg acgcagacct
      661 gctcgggggt ggtgaactgg gactctttgt tctgatcgta gaactcctgg atctcctcgt
      721 ccgagggctc cgcgtcattg gagacctgct cctgcacctt ctgaacggga aggttctcgc
      781 gtatgtcgtc gcgcaactgc tcttcggtga tgttgttctg cttcagggcc tgctcgtagg
      841 ccttctggtt gctcaggtcc tgcccggaag aacgggcctg ctcgcccacc tgtttcttga
      901 tcttctcgat ctcctggtcc acctctttgt ccgagaccgt gatgttctgc tcgctcgcgt
      961 aggctttcgc tatctcgatg ccgactagct ggggcatgat ctgctgtatg gcggccttgt
     1021 attgcgggtc atcctgcgaa agatccccga acccgctctg ctcggcgaac tggtcgagct
     1081 caccctgcgt gacgctgcca ccatcgaagg tcgctatctt cttcgcctgg gaatccgtgt
     1141 tggtctgcgg gtcggccgcg ttgcaaccaa gagaggcgaa gagaaccacc aagagaaaga
     1201 cggagacgta caacaccctg ttgaagagaa gcttgcccaa gacgaccgag aacctcacta
     1261 ccttatagaa gccaaaaccg cccgggatta tagcacgcgc aatggagcaa tcagccatca
     1321 gcaatcagcc atcagcttcc cctgctggct gctgaaagct tacggctgat agcttgcgag
     1381 cgccctagcg caagcggatc agcgcgagcg gatcacggcc cgcagggtct cctcggcgag
     1441 cgttagaggg tcctgttcgt ctgcgcgaac ggagatcctg cccgtcctcg tggtgacggt
     1501 accgccggtc gcccttctta tgagggcggg ggctgcagag ccggcggtgg cacccgtgac
     1561 ggtgaggttc cccgagcgga aggtcacgga gctggcacct gactcccgag cgaggatctt
     1621 gacccgcgag aggccgagga ggttcctcga gggttccggc agctccccga accggtcgac
     1681 gagctcctcg gccagatcct ccacctcggc gagggacctc aggccggaag ccctgcggta
     1741 gaggtcgacg cgttcgatct cgtccatcac gtacccgggc ggcagatagg cgtcgagggt
     1801 aagctccacg attacgggcc gctcctcggc acgctcctgc ggctcgccct tcgccgccgc
     1861 gacggcctcc tccaggaggt tcaggtacat ctcgaaacca acggccgcaa tgtgcccgga
     1921 ctgctcggcc ccaaggaggt tgccggcgcc acggatctcc aggtccctca tggcgatcgc
     1981 gaagccgctc ccgagctccg tgaagtcaag cagggcctcg aggcgcttct gggactccac
     2041 cgtcgcgcca acgggggcga agaggtaggc gtatgcctgc tgcctggagc gcccgatcct
     2101 accccgaagc tggtagagct gggccaggcc catcgcgtcg gaacgatcca cgataagcgt
     2161 gttcgcggtg gccacgtcga gtccgctctc gacgatggtg gtggtgacga gaacgtcggc
     2221 ctgcccgtcg aggaagtgct gcatgacccc ttcgagcgcc cgctccggca tctgcccgtg
     2281 cgccgttacg aagcgggccc ccggcacgag ctcgcgcagc ctctccgtga ccgcgtcgat
     2341 gctctccacc cggttgtgca cgaagaaggt ctgcccgccg cgcgccacct ccctctctat
     2401 ggcgttcttg accagatcct cgtcgtaggg tccgacgtgg gtgaggatgc tgcggcgtcc
     2461 ggcggggggc gtctcgatca cgctgatgtc acgtagcgag gcgagcccca tctgcatggt
     2521 gcgcgggatg ggggtggccg tcagcgtgag caggtccacc gaggccttgt actccttgat
     2581 gcgctccttg tgcttgacgc cgaagcgctg ctcctcgtcg accaccacga gccccaggtc
     2641 ctttggtgct atctgcgacc cgagcaggga gtgcgtaccg acgagaacgt ccacttcccc
     2701 ggccgcgaag tcggccagta tcctgcgcct ctgtgcctgc ggggtgaagc gtgagaggct
     2761 ctcgaccctg acggcgtagg cgccgagccg ctgcctgaac gtacgctcgt gctgctgcac
     2821 gaggatggtg gtcggggcga gcatcatggt ctgcttgccg gcgagcgcgg ccttgaaggc
     2881 ggccctcacc gacacctcgg tcttgccgaa accgacgtcg ccgcagacca ggcggtccat
     2941 ggggtgcggc ctcttcatgt ccgtcttgac ggcggtgatg gcggcttcct ggtcgggcgt
     3001 ctcctggtag gggaagctct cctctagctc gagctcccac tctccgtcct cggggaaggc
     3061 tatgccaggg gccgtcgccc tctgggcgtg caggcgcagt agctcgcccg ccaatgcctt
     3121 gaccctcttc ctgaccctgt ccgtgacctg cgtccacgtc gcgccaccga gtttgtcgag
     3181 gcgggccccc tcccccccga cgtacttgtg caagagctcc atctgctcgt agggaacgaa
     3241 gagcgtatcc ccgccacggt agccgacgcg catgtagtcg cgggtcgcgc cgagaacggt
     3301 ctgggaaacg agcccctcga aacggcctat cccctgcatc tcgtgcacca cgaggtcccc
     3361 gaccttcagg tcggcgaagg agcccaggga gcgtcccccc gaacggcgcc gcgactcccg
     3421 cctgcgcccg agcagcgagt ccctgcagaa gacggcgatg ccgccgtcgg gataggtgaa
     3481 gccttcttcc acctcgcccg gaaccgcgta gagaccaggg ggcaacgtcg catcgacccg
     3541 ctcggcctcc cgcatctggc gatttatctc cccgaaggcg tagatggtgc gcctcgcctc
     3601 cccgacggaa gagcaggcga caaagaccgc gagcccgtcc tccacgaggg tgtccagcct
     3661 cctggcggcc tccctgaggg tgtcggagaa ggccacgacc gggggggcgg agacagcctc
     3721 cccctcctcc ccgagggaga actccacgtc gggcgccggc ggctccgtgt cgtagaggtc
     3781 gggaacgaac ccttctatct cctcgggaag gtcctcctgc ggctcttcgc gccagacctc
     3841 cacgccttcg gggagaagcc cgcgcgggga gacggggtcg aggccgagca ataaccggtc
     3901 caggccgggg actcgcacgc cgcggcgtgc ctcttctggg aggccatcat ctgtggccgc
     3961 gagagcggcg agatctcctt cccgcgcggc gaagaccgtg acggcctcca gctcgcgcac
     4021 gacgcgctgg gtcgccaggg agaccgcgcg gacgctctcg acctcatccc cccaccattc
     4081 caccctgatg ggcgaccggc gggtgctcgg gaagatgtcg acgatcccac cgcgcaccgc
     4141 gaactcgcca ggcctcgagg ccctgtccac ccgctcgtag ccgagggcga ccagccgctc
     4201 cagcgtcgcg tcgagctcta tctccgtctc ggcggcgaga tagagcggct cataaagcgg
     4261 tgtccgctcc atgagagcta gtggcccggc caccaccaca tgggccgaac ggagcgagcg
     4321 cagcgacctc tgacgccgcc cgacgcgtgt taccgcaggt tcgaacacgt caccgtaaaa
     4381 gacaccgcgg gatggaaggt gcacgactag gccgtcggtg aagacggcgg cctcacgcgc
     4441 gtaccgctcg gccgcctcct cgccagcggc gagtaggagc acgggacggt cggcggtcgc
     4501 cgccaggtag gcgcgtaccc tgcgcggggc acgaagaagt ctcgattgcg tcctttcgct
     4561 caccggtaga ttctaccccc aaaggtcgaa gttcatgtga cgggatccac ccctgaccag
     4621 cacctctggg ataatcttcc gggtcgaaac acgaacactg gaggatcttc ctatgaccgt
     4681 tctagacgag ttgaacgaac ggggcgccgg taccctgccc ggcctgatcg ggctggagat
     4741 aaagggggcc gaggagggtg taatctcgag ccgcctcgca ctccgagacg agctcatggc
     4801 cccgaacggc tacctgcacg cggccacggt ggtggccctc gctgatacct cctgcggcta
     4861 cggcacgttc gtcaacctgc ctgacggcgc cgagagcttc accaccatag agctcaaaag
     4921 caacttcgtc ggaacgaaac ggggaggcgc gatctcatgc cgcgccaggc tcgtgcacgg
     4981 cggccgaacg acacaggtct gggacgcaga agtctccgac gaagagagcg gcaagccaat
     5041 agcactgttc cgctgcaccc agatgatcct ctacccacgc ggttagagct gtcagcgatc
     5101 agcggtcggc ttttctgcct ggctgctgcc ggctgacagc tgatggcttt atcccggtga
     5161 tgagcacgac gctgcagagg atgagggcaa gcccggcgat ggtgccggat ccgaacggct
     5221 caccaaggag caggacgccg aacaggagac cgaagacggg cacgagcagc gtgaccgtga
     5281 cggtgctggt ggggcccacg ttctcgatga ggcggaagta gatgaggtag gccagggccg
     5341 tcgagagcaa ggccaacccg agcatgcaca ggaccacgac caaagacggc gcctgctcgg
     5401 gaacgctggc cgcggagagc ggcaggagga atactgtagc cgccgtcaac tggccaatgg
     5461 ccataccggt cggcggtatg ccggagaagt agtgcttggc gtaggtggcc cccagcgcgt
     5521 aagagaggga cgccagcagc atcgctacca cggacagcag gacggccccg ttcaggggca
     5581 gggggtccca ccccaagaga acggcgaccc cgaagatccc caggagaatg ccgaagatct
     5641 ttttcgccgt aagggcctca cccatccaca cggcaccgac catggccgtg aacatcacgg
     5701 tggtcgagtt gattattgcc gcgagggagg ccgtcaggtg tatctcggcg gcggagatga
     5761 gcgagaaggg tacggcggag ttgaaaagcc cgaggaccag gaacgccctc caccggctgc
     5821 ggatcttcgg cacgcgcccg acggcgagcg cgtagagcgc cagcacggcc gcggcgaggc
     5881 cgacgcgtaa acccacgagc aagaaaggtc ccagggccgg cacgacgacg cgaatgaaca
     5941 ggaacgcacc gccccacaac gcgccgagca cgatgaggcc ggcgaaatcc ctgggcctca
     6001 tatggcacct acttcgagca ggaaagacat ctccaggtta gacggggggc aatgccatca
     6061 gatgcgtgag cgtgtaggac cgtggacctg atggctcatc gttccgtagc agcgagcacc
     6121 ctcgtctcgt gctcgagcag acgctccttg aggtgcaggc cgccgttgaa cccacccagc
     6181 gtgccgtctg cggcgatgac gcggtggcag ggcaccacga cgggcaggac gttgcgggcg
     6241 ttggcgctac cgacggccct cgctgcaccg ggccgcccga gtgcgtcagc cacctcgccg
     6301 taggaccttg tctcgccgaa ggggatgcgc gtgagctcgg cccacaccgc cttctgccac
     6361 tccgaaccgg agaatgccag cggcacgtcg aaggccctcc gttccccgct cgagtactcg
     6421 gccagctccg ctcgcaccgg ggccgtcttc gttgggtcgg accttacggt ataaccctca
     6481 tcttccagat cctcctccat gaccggaccg tagttgctct ccacgaagtg caaccgtatc
     6541 agggcgccgt cttcgtccac tgcgaacacg agcggaccgg cgggagaagc tacctcgtcc
     6601 acgtatgcct tcaagctacc acctctctat tctcgttccg atccggctct tctgcgaggc
     6661 tggcccacag gtgctgtgtg gcgtaggcgc gccagggcct ccacgtctcg gcgagggtgg
     6721 ccacgctccg tcggtcgtcc gggtgtccga gctttcggac ggcctgcctg accccgaggt
     6781 ccgtgggcag gaacgcgtct gggtcgccga gcgcgcgcat tgcgacgtaa gaggccgtcc
     6841 acggcccgat cccccgcagg cccagaagcc ggcgttcgac ctcgtcccgg tccgagccgg
     6901 ggtcgagcga gacgtcaccg tcaagcgcac gcgccagggt tcgcaacgcc tcggccctgg
     6961 ccgcgggcac gccgaaacgg tggaggtcgg ctccggccac gtcttcagga ccggggaaga
     7021 tgtgcgtcag cccattctcc gagagtaggg aggccgggag gggcgcgcca caccgggcta
     7081 cgagccgtcc ggcgagcgtt ctggccccgg cgacggagac ctgctggccg agcacggcgc
     7141 ggaaggcgag ctcggcgccg tccacgctgc caggcacgcg ccgcccgggt gaacggcgga
     7201 cgatcggccc gagcatcggg tcgtcgccga gcacgtcggt gacggcggcg gggtccgcat
     7261 cgaggtctag tagcctgcgg cagcgttgca ccgcggtcc
//
LOCUS       DBNGVD010000624         5568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2367927, whole genome shotgun sequence.
ACCESSION   DBNGVD010000624 DBNGVD010000000
VERSION     DBNGVD010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2367927"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC16CM_16555"
     CDS             <1..386
                     /locus_tag="AC16CM_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3141496.1"
                     /translation="QYLADMGVNIPSIDTEVAMLSGGQRQAIAVARAVYSDARMLLLD
                     EPLAAMGAKEGALILDLIQRLKEERSIPMILIVHNYAQVFDVCDRINLLRNGRIEYDK
                     PVAESSVEELTELVVSEYRKARETG"
     gene            807..1943
                     /locus_tag="AC16CM_16560"
     CDS             807..1943
                     /locus_tag="AC16CM_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3141497.1"
                     /translation="MERSRASSSNTAYTILGLVFALLVGGYFFYRISGVVLAFLLTIL
                     LSIILSAPVNYLERRGWTRTWGALAVLAVLVGLVYLFGLAIVPAVETQSREFAEAFPT
                     LLDEALSLANRAQDFFGLGTQIGLDPQSLSDLGRRALTGGTVSTAAGVGLTLATALSL
                     GVVVFISTIYLVIRPAPWVDGFVSLFPAGWRPRTREVLEAMYHTVQRWFLGQLTAMTF
                     IAVFWAISLSIIGVPFALLIGIFSGLVSFIPYLGATISVVVPILLALVSDPFTVIWVV
                     LAFIIIQQIEGNILQPIVMSRAVDLHPALVVFAILIMGTLFGLTGVFLAVPLVAALQV
                     MVRELWVKRMDEIGTDPNPPTRDQPRRTLPRPIARLFERFRKKS"
     gene            complement(2104..3087)
                     /locus_tag="AC16CM_16565"
     CDS             complement(2104..3087)
                     /locus_tag="AC16CM_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3141498.1"
                     /translation="MYGYRPTTRLLSMLELLQARGKMGGPELARRLEVGERTVRRYVA
                     MLQEMGVPVEAERGRYGAYVLRPGFRLPPMMFTDEEALALALGLLSARRLGLDGVAPA
                     VEGAQAKLERVMPDELRERVRTFEEVVIPATTPERLPAERIVVTISGAVRERKRVWIR
                     YRSDSGDETERSIDPYAVVRGDAFWYAFGYCHLRQARRLFRLDRVASVEVLDPSFEMP
                     PGFDTPEGVLGAIADMREDSWSVEVLLETTIGEARGQLPKMGVSLQETPEGVLMRSST
                     SDLAWMSRVLASLSFPYVVRSPSELRGALQRLAAEISTIAERAEDSERERR"
     gene            3447..3668
                     /locus_tag="AC16CM_16570"
     CDS             3447..3668
                     /locus_tag="AC16CM_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141499.1"
                     /translation="MHEWISVELSRGRNADVMAEAEERRLARLAPRRAAWPVLRARVA
                     RRVFSFALALEREETWRAVWEKLEAPRHP"
     gene            3781..4638
                     /locus_tag="AC16CM_16575"
     CDS             3781..4638
                     /locus_tag="AC16CM_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3141500.1"
                     /translation="MEANSLEVVGIGLIGRIDALEVGGRSLAFWGLGQVGVAIKGPEG
                     VLYVDPYLTDSDGEGGSLPRTYPPPVGPDEVTNASAVLLTHAHIDHTDPETVLPLAEA
                     SPEASFVAPFTSRDTLVEAGLDEGRLVVPEVGEPFEVSGATVTAVPSAHTGLEHDPDR
                     GYPYLGYVIEWNDVTVYHAGDTVVYDGLVETLSDWDIDLAFVPINGRDYFRTQRNIIG
                     NTDFREVAELTEVLDFGLIVPTHYDLIEGNTADPGHFVSHLYNLNPMRPQKLLRPGEL
                     LLFVRETGR"
     gene            complement(4635..>5568)
                     /locus_tag="AC16CM_16580"
     CDS             complement(4635..>5568)
                     /locus_tag="AC16CM_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HIR3141501.1"
                     /translation="VVRVEAAGVSFAEVQMLRGRYFSQPKFPFVPGYDLVGRVEKVGD
                     GADGWLVGCRVAALTETGAWADRVTLGASELVVVPEGLPPADAVAVVTNGVTAWQMVH
                     RAARVRPGQTVVVHGASGGVGTLLVQLARLAGATVVGTASASKHGEVHALGAIPIDYR
                     TEHLTERVRELSPGGVDAVFDHVGGSGLVGSWRMLRKGGTLVSYGSASTLGDGGHRLQ
                     PYLPIVGRIMLWSVLPNGKRATFYYVKRWPTRFQQDLATVLALLSEGKIEARVTEQMP
                     LERAAEALGLLASGKASGKVVLVTEVDDQDAQPS"
BASE COUNT          975 a         1856 c         1725 g         1012 t
ORIGIN      
        1 cccagtacct cgcggacatg ggcgtcaaca tcccttccat agacaccgag gtggcgatgc
       61 tctcgggtgg gcagcgccag gccatagccg tcgcgcgcgc ggtctactcg gacgccagga
      121 tgctccttct ggacgagccg ctggcggcga tgggggccaa ggagggggcc ctaatcctcg
      181 acctcatcca acgcctcaaa gaagagcgga gcatacccat gatcctgatc gtccacaact
      241 acgcccaggt cttcgacgtc tgcgacagga tcaacctgct aagaaacggc cgtatagagt
      301 acgacaaacc cgtcgccgag agctccgtgg aggagctgac cgagctggtg gtctccgaat
      361 accgtaaggc cagggagacc ggctagcgcc ggtcagcacg ctccggctaa cgcctccgct
      421 tgtcggcaac gcttcggctt cgcctcgctt tcggctgata gggttgcggc ctgactcttc
      481 catacgacgg attatcggtg cggatcgagg agatctcggc ttgttaagtt gacctggaga
      541 agggcgcttt tcggtcgcat gcatttgccg cagattcttc ctccgtcatt cccgcgaaag
      601 cgggaaccaa cgggtatcag gagagtaaca attgcttgct actggaatcg ggtaccgtgc
      661 aagcgttcgg cgaccagcaa gaccagaagc tgatagctga cggctgatag ctgacagccc
      721 ccagcttctg tagtcacaat agcgggtttt gggtatgctg gccggagcaa gtagagttct
      781 ttatgagtcg agcccaagga gtaacggtgg agaggtctcg ggcctccagc agcaacacag
      841 cgtacacgat cctcgggctg gtcttcgcgc tgctcgtcgg ggggtatttt ttctaccgga
      901 tcagcggcgt ggtcctggct ttcttgctga ccatactgct ctccataatc ctgagtgccc
      961 cggtcaacta cctcgaacgg cgcggatgga ccaggacgtg gggagccctg gccgtgttgg
     1021 ccgtgctcgt cggcctggtc tatcttttcg ggctcgccat cgtcccagcc gtcgagacgc
     1081 agtcccgcga gttcgccgag gccttcccga ccctcctgga cgaggccctc agcctggcca
     1141 accgggccca ggacttcttc gggctcggca ctcagatcgg gttggatccc cagagcctgt
     1201 cggacctcgg gcgtcgggcc ctcactgggg gcacggtgtc cacggcggcc ggggtcgggc
     1261 tgacgctggc gaccgcgctc tcgctcgggg tcgtggtctt catctccacg atctacctcg
     1321 tcatccgccc ggcgccgtgg gtcgacgggt tcgtctcgct ctttcccgct gggtggcggc
     1381 cgaggacgcg ggaggtgctg gaggccatgt accacacggt gcagcggtgg ttcctcggcc
     1441 agctgaccgc gatgaccttc atagctgtgt tctgggccat ctccctctcc atcatcgggg
     1501 tgcccttcgc actgctcatc ggcatcttca gcggcctcgt ctccttcata ccctacctag
     1561 gcgcgaccat ctcggtcgtc gttccaatac tactggccct ggtctccgac ccgttcacgg
     1621 tgatctgggt ggtcctcgcc ttcatcatca tccagcagat cgaaggcaac atcctccaac
     1681 ccatagtgat gagccgggcc gtcgacctgc accctgccct ggtggtcttc gcgatcctta
     1741 tcatgggcac cctcttcggc ctgaccgggg tcttcctggc cgtaccgctc gtggccgccc
     1801 tgcaggtaat ggtgcgggag ctatgggtga agaggatgga cgagatcggc accgacccga
     1861 acccgccgac cagggaccag cccagacgca ccctgccccg cccgatcgcc aggctcttcg
     1921 aacgtttccg gaagaaatcc tgagagaagg tttcaggctg aaggcatcag gttacaggtg
     1981 tttttcctgt tgccttcgcg ggccgagaac ccctgtaaaa cagcagcttg ctagacgacc
     2041 ggcgtcaagc gaatagagcc ctcagccaaa agctgatagc tgacagctgt caactggcct
     2101 gtctcatcgc cgttctcttt ccgagtcttc ggcgcgctcg gctatggtag agatctcggc
     2161 ggcgaggcgc tgcaacgccc cgcgcagctc ggatggactg cgcaccacgt aagggaacga
     2221 gaggctggcg aggacgcggg acatccacgc gaggtcggag gtcgaggaac gcatgaggac
     2281 cccctctggc gtctcttgaa gtgagacacc catcttcggg agttgcccgc gcgcctcccc
     2341 tatcgtggtc tcaagcaaca cctccacgga ccagctatct tcgcgcatgt cggcgatggc
     2401 cccgagcacg ccttcggggg tatcgaagcc ggggggcatc tcgaaagagg gatccagaac
     2461 ctctaccgag gcgacccggt ccagccggaa caggcgtctt gcctgccgca ggtggcagta
     2521 gccgaaggcg taccagaagg cgtcaccccg aacgacggcg taagggtcta tcgaccgctc
     2581 cgtctcatcg ccggaatcgg agcgataccg tatccacacc cgctttctct cccgcacggc
     2641 gccgcttatg gtcaccacga tcctctccgc gggaaggcgc tctggggtcg tggccgggat
     2701 cacgacctcc tcgaaggtcc ttacacgctc gcgcaactca tctggcatca cgcgctccag
     2761 ctttgcctgg gcgccctcga cggcgggggc cacgccgtcg aggccgagtc tgcgggcaga
     2821 gagcaggccg agggcgagtg cgagcgcctc ctcgtccgtg aacatcatcg gcggcagccg
     2881 gaaacccggc cgcaggacgt aggcaccgta cctgcccctc tccgcctcca cgggcacgcc
     2941 catctcctgc agcatcgcca cgtagcgacg cacggtcctc tcgcccacct cgagccttct
     3001 ggcaagctcg ggcccgccca tcttcccccg cgcctgcagc aactccaaca tcgagagcag
     3061 cctcgtcgtc gggcggtaac cgtacactag ctcgctccct tcggtcgctc aaagcacgtt
     3121 gcggcccttc gggcctgcac ttgtcggcac gctcgggctg gcgccctcgc ttgtcggcaa
     3181 cgctttggct tcgcctcact ttcggccggt cgtctctctc cgtaacggtg gcttcggcta
     3241 cccacttttt ccgggcatcg aatcttcaga gcggcggggt gcaggccgca gcgagatctg
     3301 gctgaaagcg agccctgtaa gggcgaagcg ttgctgacaa gcgaaggcgt cagccggagc
     3361 gtgctgaaaa gcttcttcat caggaaggat tatgaccgct ttcggctgta ctctcatcgg
     3421 gtaatcaagc gagggagatg aacggcgtgc acgagtggat ttcggtggag ctgtcgcggg
     3481 gaaggaacgc cgacgtcatg gcggaggccg aggagcggag gctggcgcgg ttggccccgc
     3541 gtagggcagc ctggccggtt ctgagggcgc gagtggcgcg gcgggttttc tcgtttgcgc
     3601 tggcgctgga gagggaagag acctggaggg ccgtctggga gaagctggag gcaccccggc
     3661 acccgtagaa cacgctggcg gaccaggaat ttcgaggtag gaatgcaggg ccccagaccg
     3721 cgcgcccgtt accatgttcg cccccccgaa tctccgaccc gctcctgtgc tagtgtgggc
     3781 atggaggcaa actctctgga ggtggtcggg atcgggctga tcggcaggat cgacgcgctg
     3841 gaggtgggag ggcggtcgct cgccttctgg gggctggggc aggtgggggt ggcgatcaag
     3901 ggccctgaag gcgtcctgta cgtggaccct taccttaccg actcggatgg tgagggtggc
     3961 agcctgccgc gcacctatcc cccacctgtg ggccccgacg aggtaaccaa cgcctcggcc
     4021 gttctcctta cccacgccca catcgaccac accgatccgg agaccgtctt gccgctggct
     4081 gaagcctcgc ccgaggccag cttcgtggcg cctttcacga gccgagacac gctcgtcgaa
     4141 gctgggctgg atgaaggtcg cctcgtcgtg ccggaggtcg gggagccttt cgaggtctcg
     4201 ggggcgacgg tgacggccgt cccttccgcc cacacggggt tggagcatga tcccgatcgc
     4261 ggttatccgt acctcgggta cgtcatagag tggaacgacg taaccgtcta ccatgccggc
     4321 gacaccgtgg tgtacgacgg cctggtcgag accctctcgg actgggacat cgaccttgcc
     4381 ttcgtcccaa tcaacggccg cgactatttc cggacgcaga ggaacatcat aggcaacacg
     4441 gacttccgcg aggtcgcgga gctaaccgag gtactggact tcggcctgat cgtaccgacg
     4501 cattacgacc tgatagaagg caacaccgca gaccctggac acttcgtcag ccacctctac
     4561 aacctgaacc ccatgcgccc gcaaaagctt ctccgccctg gcgagcttct cctgttcgtg
     4621 cgtgagacag ggcgctagga cggctgagcg tcctggtcgt ctacctccgt aacgagcacc
     4681 accttgccgg acgctttgcc cgaggccaga aggccaaggg cttcggcggc cctctccagc
     4741 ggcatctgtt cggtgacgcg ggcttcgatc ttcccttccg atagaagcgc gaggaccgtc
     4801 gcgaggtcct gttggaacct tgtgggccaa cgcttcacgt agtagaacgt cgccctcttg
     4861 ccgttgggca gtacgctcca gagcatgatt cggccgacga tcggtaggta gggctgcaaa
     4921 cgatgccccc cgtccccaag cgtggacgcg gaaccataag agacgagcgt gccgcccttc
     4981 ctcagcatcc tccacgaacc gacgagtcca gatcctccca cgtgatcgaa aacggcgtcc
     5041 accccgccgg gggagagctc acgcaccctc tccgtcaggt gctctgtgcg gtagtcgatc
     5101 gggatggccc cgagggcatg cacctccccg tgcttggatg cggaggcggt cccgaccacg
     5161 gtcgcaccgg ccagccgtgc gagctgcacc aacaaggtgc ctacgccgcc agaagccccg
     5221 tgcacgacca ccgtctgccc cggcctcacc ctcgcggcgc ggtggaccat ctgccaagcc
     5281 gtcaccccgt tggtaaccac ggccaccgca tcagccggcg gaagaccttc gggcaccacc
     5341 acgagctctg aggcgccgag cgtgacgcgg tcggcccacg cccccgtctc cgtgagcgcc
     5401 gccacccgac accccaccaa ccatccatca gcaccatcgc caactttctc aaccctaccc
     5461 acaaggtcgt agccagggac gaagggaaac ttcggctggc tgaagtagcg tcccctgagc
     5521 atctgtacct cggcgaaaga gacacccgca gcctccaccc gcacgacg
//
LOCUS       DBNGVD010000625         3353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_2913286, whole genome shotgun sequence.
ACCESSION   DBNGVD010000625 DBNGVD010000000
VERSION     DBNGVD010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_2913286"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..843
                     /gene="hisB"
                     /locus_tag="AC16CM_16585"
     CDS             259..843
                     /gene="hisB"
                     /locus_tag="AC16CM_16585"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3141502.1"
                     /translation="MARKGSAERKTAETYVHVDVDVDGDGRVEVETGVGFLDHLLHLF
                     AHHSGVDLTVEAKGDTWVDDHHTVEDTGLVLGRAFDEALGDRGGIVRFADASAPLIEA
                     LSTAVVDLGGRSHLTCNLGPMPEKIGTFDVELFPEFLRAFTQYGRFTLHFNCLYGENA
                     HHKIESGVKAMAVAVREGVQFRTSGSASTKGVID"
     gene            946..1569
                     /gene="hisH"
                     /locus_tag="AC16CM_16590"
     CDS             946..1569
                     /gene="hisH"
                     /locus_tag="AC16CM_16590"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3141503.1"
                     /translation="MADSLRVAVVDYDAGNTLSVTRALEKVGARVELTPDPDRVLAAD
                     ALVLPGVGAFGDCMRKLRERGMDEACSEAFRRGTPFLGVCVALQVVFERSEETPGVEG
                     LGLMPGGVVRFGGDGLKVPHMGWNELSLVRGHPVLDGLDGEAFYFVHSYYPEPDEPAD
                     LVGETEYGVKFCSAAGRENLVAVQFHPEKSSISGLRLYENFLSWAGA"
     gene            1574..2179
                     /locus_tag="AC16CM_16595"
     CDS             1574..2179
                     /locus_tag="AC16CM_16595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3141504.1"
                     /translation="MGQNRTTAGDLLRSSRLRLRPLEREDLPRYQELFSEPEISLHYG
                     GLGVPESLSRLQRRFDGGEFDVTDRSLHLVIETEGQMIGATVLSNDENLPSRSATFGI
                     IIGDRAYLDRGYGSEASTLLLDYAFAVLGYHKVNLDLFEYNVRARVMYERLGFVLEGR
                     RRENHWTRGRFWDDILMGVTAEEWWAEHGKPQPPELGSVRR"
     gene            2176..2904
                     /locus_tag="AC16CM_16600"
     CDS             2176..2904
                     /locus_tag="AC16CM_16600"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3141505.1"
                     /translation="MSSEVPFTVFPAIDLRGGKCVRLKQGDFGRSKEYDADPVQRARE
                     WERQGAEAIHVVDLDGAEQGSPVQLELVERMAGAVDVPLQVGGGIRSLNDLRAARASG
                     AERVVVGTGAVGDRRLRLAAVEDAGPSLVVAVDARDGIVATHGWRQSSGIPVLDLAEE
                     LAADGVASVLFTDVARDGMDAGAALEATADVARAIPTIASGGVRGVEDVAALSEIPGV
                     VGVVVGTALYEGQVTLAQLMAARR"
     gene            complement(2947..3255)
                     /locus_tag="AC16CM_16605"
     CDS             complement(2947..3255)
                     /locus_tag="AC16CM_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3141506.1"
                     /translation="MPTLDSTVRRTGDYESRTEEYELIYSVGASPSRSLNIKYQAARA
                     YEINAALDAAVETAREAIADHDAGRKGPTLEALAKFSWRAVQAAKYEEGEPVASGLEY
                     "
BASE COUNT          588 a          991 c         1170 g          604 t
ORIGIN      
        1 aggagcgtca gaatgaggtt gtgcggtacc ggatcggcgg ccaggaactc cccggcctcg
       61 tggagagccc aaccgggatc ttcgccaaga accacttcga gggacatggc tggattcttg
      121 cacgtccctg acgcaaagtc tcgaacgtgg aagagttcgt ctcgcggaag gactcgaacg
      181 cgcgaacctt cggcgtcagc cgttgtcgaa gccgaaacgt cagctagaat gctctgaatc
      241 tagtgatgcg ggaggaagat ggcgcgaaag ggcagtgcgg agcgcaagac ggccgaaacc
      301 tacgtccacg tcgacgtcga cgtcgacggt gacggacggg ttgaggtcga gaccggggtg
      361 gggtttctgg atcacctcct gcacctgttc gcccaccact cgggcgttga cctcaccgtc
      421 gaggcgaagg gcgatacctg ggtggacgac caccacacgg tggaggacac ggggctcgtg
      481 ctaggccgag ccttcgacga ggccctcggc gaccggggcg gcatcgtgcg ctttgcagac
      541 gcgagcgcgc cgctcatcga ggccctcagc acggcagtag ttgacctggg aggaagaagc
      601 cacctcacgt gcaacctcgg gccgatgcca gagaagatag gaaccttcga cgtggagctc
      661 ttccccgagt tcctgcgtgc cttcacccag tacgggcgct tcacgctcca cttcaactgc
      721 ctctacggcg agaacgccca ccacaagata gagtcgggtg tcaaggcgat ggcggtcgcc
      781 gtaagagaag gggtgcagtt ccgcacctcg ggcagcgcgt ctactaaggg ggttatcgat
      841 tagcggtcag cctttagccg ttagctatca gcggtcagct cccaaccagg aaagaatccg
      901 atcatcaggt acgaagatca agacgaagct gaaagctaga ggctgatggc tgatagctta
      961 cgagtagctg ttgtcgatta tgatgcgggc aacactcttt ccgtcacgcg tgcgctggag
     1021 aaggttggtg ccagggtcga gcttacgccg gatccggacc gggtgctcgc cgccgatgcc
     1081 ctggtgttgc ccggggttgg ggctttcggg gactgcatga ggaagctgcg ggagcggggg
     1141 atggatgagg cttgctcgga agcgtttcgg cggggcacgc cgtttctcgg ggtttgcgtg
     1201 gcgctgcagg tcgttttcga gcgctccgag gagacgcccg gcgtggaggg gctcggtctc
     1261 atgccaggcg gtgtggtgcg cttcgggggc gacgggctga aggtcccgca catgggatgg
     1321 aacgagctat ccctggttcg cggccacccc gtcctggacg gtctcgacgg cgaggcgttc
     1381 tacttcgttc actcgtacta cccggaacca gatgagcctg ctgacctggt cggcgagacg
     1441 gagtacggcg tgaagttctg ttcagcggcc gggagggaga acctggttgc cgtgcagttc
     1501 caccccgaga agagcagcat ctccggtctg cggttgtacg agaacttcct ttcgtgggcg
     1561 ggggcataga caggtggggc agaaccggac gacggctgga gatctcctcc ggagttcccg
     1621 tcttcggcta cgtccgctgg aaagggagga cttgccacgc taccaggaac ttttctccga
     1681 gccggagatc agcctgcact acggcgggct cggcgtgcca gagagcctct cccggctgca
     1741 gcggcgcttc gacgggggag agttcgatgt cactgaccgt tccctgcacc tggtgatcga
     1801 gaccgaaggc cagatgatcg gggcgacggt gttgagcaac gacgagaacc ttccgagccg
     1861 ctcggcaacg ttcggcatca tcatcggcga ccgggcttat ctcgaccggg gctacggtag
     1921 cgaggcgagc acgctgctcc tcgactacgc ctttgcggtt ctcggatacc acaaggtgaa
     1981 ccttgaccta ttcgagtaca acgtccgcgc cagggtcatg tacgagaggc ttggtttcgt
     2041 gctggaaggg cgccgcaggg agaaccactg gacgcgaggc cgcttctggg atgacatcct
     2101 catgggcgtt accgccgagg aatggtgggc agaacacggt aagccacaac cgcccgaact
     2161 cggctccgtg cgccgatgag cagtgaagtc cccttcacgg tcttcccggc gatagacctg
     2221 cgcggcggca agtgtgtgcg tctcaaacag ggcgatttcg gccgcagcaa agagtacgat
     2281 gcggacccgg ttcaacgggc ccgcgagtgg gagcgccagg gggctgaggc catccacgtc
     2341 gttgacctcg acggcgccga gcagggtagc ccggttcagt tggagctcgt ggagcgcatg
     2401 gctggtgcgg tcgatgttcc gctacaggta ggaggcggca tcaggagcct gaacgacctg
     2461 cgcgcggccc gggcgagcgg tgcggaacgc gtcgtggtcg gcaccggcgc ggtcggcgac
     2521 aggaggctac ggctggcggc ggtcgaggat gccggcccct cgctggtcgt cgcggtcgat
     2581 gcgagggacg gaatcgtggc cacgcacggc tggcgccagt ctagcggtat ccccgtactc
     2641 gacctcgccg aagaactggc cgccgacggc gtggcctctg tcctgttcac ggacgtggcc
     2701 cgtgacggca tggacgccgg tgcagccctc gaggcgacgg cggatgttgc gagagcgatc
     2761 cctaccatcg ccagcggcgg cgtccggggt gtcgaagacg ttgccgccct ctccgagatc
     2821 cccggcgtgg taggtgtcgt ggtcggtacc gccctctacg agggccaggt cacgttagcg
     2881 cagctaatgg cggcgcgtcg ctaggcccat ccgccgtagg ggccgttcgc gagccgccct
     2941 ggtcgttcaa tattccaggc cagaggctac gggttcgccc tcttcgtatt tggcggcctg
     3001 caccgcgcgc caggagaact tggccagggc ttcaagggtg ggacccttcc ggcccgcgtc
     3061 gtggtcggcg attgcctccc tcgccgtctc gacggccgcg tcgagggctg cgttgatctc
     3121 gtaggcccgg gcagcctgat acttgatgtt gaggctgcgg gagggcgagg cgccgacgga
     3181 gtagatcagc tcgtactcct ccgtgcggct ctcgtagtct ccggtgcgcc tcaccgtgct
     3241 atccaaagtt ggcatgcctg catcctatca tctgcgcgct tcccgtcccc gataaggttg
     3301 gggtagacta cggcaggagg gccgtgaacg atttcttcga gaccagaaag aag
//
LOCUS       DBNGVD010000626         3629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3460380, whole genome shotgun sequence.
ACCESSION   DBNGVD010000626 DBNGVD010000000
VERSION     DBNGVD010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3460380"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..830)
                     /locus_tag="AC16CM_16610"
     CDS             complement(228..830)
                     /locus_tag="AC16CM_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR3141507.1"
                     /translation="MATGSGDEGRGSIFRFDTRQIVVAGIIGGIALFLGFTRLGLIPV
                     PIPLIGNATIMHIPAIVGGVLEGPIVGLLAGAIFGVFSFLQADNPVFLNPVVAIVPRL
                     FIGVVAWAVFVALKRWSVDLAAVVAGLLGSFANTVGVLGLAILFGLLPVAVIPAILPQ
                     AIAEAVLAAVVTLVIVRGVMLFRSGRTTAPETGSDEERRY"
     gene            complement(982..1764)
                     /locus_tag="AC16CM_16615"
     CDS             complement(982..1764)
                     /locus_tag="AC16CM_16615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3141508.1"
                     /translation="MYDYRSMFDLDGKSALVVGAGSGIGEASAQGLAAFGASVVCADM
                     NGEAARNTAEEISSGGGAAEALELDMTDPRSVDAAVERVGTPDVLVSTPSLNVRKPVL
                     EITDEEFDRIIDLNLKGTFRLIRNFGRGMAERGSGSIIAFSSIRGQVVEPGQGIYAAT
                     KAGTVQMLRALASEVGPRGVRVNAIAPGVVETPLTAQIKDSPDWYDSYAAKSILGRWA
                     QPHEMVGMVVYLASEASSYVTGGFMLVDGGWTAADGRFTPPL"
     gene            complement(1757..2995)
                     /locus_tag="AC16CM_16620"
     CDS             complement(1757..2995)
                     /locus_tag="AC16CM_16620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3141509.1"
                     /translation="MFESVLEDVLAGIDREELVRLAGDLVRIPSVYRPGEEDGNEGDV
                     AGFVADYLRNSGFEARTEDVAPGRPNVWVVWEGERPGKTLLFEAHTDVVTEGNAQEWR
                     HPPFAAEREGGRLYGRGACDTKGNLAAALVAVRAIKDSGVSFPGTLVLCHPVDEEGMM
                     TGIKTFIENGHAGGVDAAVICEPEENQLCVKQKGALRVEVTVRGRMAHGAMPLSGVNP
                     VTRAARFVVAVEELEKEEIGRHGEDPFLGYPSLTPTILMGPDYGDPQINVIPASTYVA
                     LDIRTVPAQSHERLVGRLEDIIARLGSEDPDFDATLEVIEERPPTETPEDEPLVAAMA
                     RAHRHLTGKEPRYNGVPGATDGTFLSAWAGIPIVTTGAGEREVPHHKDEWVDEDELFA
                     ACRLYAATAMYYAYGEEAHV"
     gene            3211..>3629
                     /locus_tag="AC16CM_16625"
     CDS             3211..>3629
                     /locus_tag="AC16CM_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3141510.1"
                     /translation="MRVSEAIGRVLVDNGVGAFFGLAGSGNFAVLNDLHEAGAAFYSS
                     RHEAGAVMMADGYARASGKPGVASVHQGPGFTNALTGLAEAAKARTPLLLLAADIPAG
                     TLWSNFKVDQGSLAATLGAIPERVRTPATAAEDTARAL"
BASE COUNT          593 a         1228 c         1152 g          656 t
ORIGIN      
        1 ctctatcttc aggttcaccc ccccgagcgc gggtaccgcg tcggcctccg ccgtcctgta
       61 ggtgaacgag aggttatcta gctcgatcaa ggcgccgcac cgcaactata acggttctcg
      121 ttgggggtga gacacgaaga aggctacagg tttcgggctt aaggtcattc atcctgttgc
      181 ctgttgcctt tactcacctc aggttcgctc tctcccagag aatcgctcta gtagcgacgt
      241 tcctcatctg atccggtctc cggggctgtc gtcctgccgc tgcggaagag catcacgccc
      301 ctcactatga cgagggtgac cacggcggcc agaacggcct cggctatagc ctgcggcaag
      361 atggccggga tgacggccac cggcagcaaa ccgaacaata tggcgagacc cagaacccca
      421 accgtgttgg cgaaggagcc gagcaacccg gccacgacgg cggcgaggtc cacgctccag
      481 cgcttgaggg caacgaagac ggcccacgcc accaccccga taaacaggcg cggcacgatc
      541 gcgacgacgg ggttcaggaa cacggggtta tccgcctgca gaaaagagaa cacgccgaag
      601 atcgcgccgg ccagcagccc tactatggga ccctcgagca ccccgccaac gatcgccggt
      661 atgtgcatga tcgtggcgtt cccgataagc ggtatgggca ccgggatcag gcccaaccgc
      721 gtgaacccga gaaagagcgc gatgccaccg attatgccgg ccaccacgat ctgcctggtg
      781 tcgaaccgga atatggagcc ccgtccctcg tctcccgaac ccgtcgccat gctctctcct
      841 tctctcgtgg tacccgccgt cggccaggct atgtgctggg gtcagctagt atctgcgcga
      901 gtgcgcatcc tgtcaaacgc aaggagcaga ttgcaagccg aaagctgaac gctgatagct
      961 gaccgctgat ggctaatagc cctacagtgg cggggtgaat cgtccgtcgg cggcggtcca
     1021 gccgccgtca acgagcatga agccgcccgt gacgtaggag ctagcctccg aggcgaggta
     1081 gacgaccatg ccgaccatct cgtgcggctg ggcccagcgg ccgagaatgc tcttggcggc
     1141 gtaagagtcg taccagtcgg gggagtcttt gatctgggct gtaagcggcg tctcgacgac
     1201 gccgggcgct atggcgttca ccctgacgcc gcgcggccct acctcggagg cgagggccct
     1261 cagcatctga accgttccgg ccttggttgc ggcgtagatg ccctgtcccg gctcgacgac
     1321 ctgcccgcgg atgcttgaga aggcgattat gctgccggag ccgcgctcgg ccatgccgcg
     1381 cccgaagttg cggatcaggc ggaacgttcc cttgaggttc aggtctatta tcctgtcgaa
     1441 ctcctcgtcg gtgatctcca gcacgggttt tcttacgttg aggctgggcg tgctgacgag
     1501 cacatcgggg gtccccaccc gctcgacggc cgcatccaca ctccgggggt ccgtcatgtc
     1561 gagctcgagg gcttctgccg cgccgccccc cgagctgatc tcctcggcag tattccgggc
     1621 ggcctcgccg ttcatatccg cgcacacgac gcttgccccg aaggcggcga ggccctgggc
     1681 gcttgcctcg ccgatgccgc ttccggcacc gacgacgagc gcgctcttgc cgtcgagatc
     1741 gaacatggag cggtagtcat acatgggctt cctccccgta ggcgtagtac atggccgttg
     1801 ctgcgtagag ccggcaggcg gcgaagagct cgtcctcgtc gacccactcg tccttgtggt
     1861 gcggaacctc gcgctcgccg gcccccgtgg tgacgattgg gatgccggcc cacgcgctga
     1921 ggaacgtgcc gtcggtcgcg ccgggcaccc cgttgtagcg cggctctttg ccggtcaggt
     1981 gtctgtgggc cctggccatc gcggcgacca ggggctcgtc ttctggggtc tcggtcggtg
     2041 ggcgttcctc tatcacctcg agcgtcgcgt cgaagtccgg gtcctccgac cccagccttg
     2101 cgattatgtc ttccaggcgg cccaccagcc tctcgtggga ctgggccgga acggtgcgta
     2161 tatcaagggc gacgtaggtg ctggcgggga tcacgttgat ctgggggtcc ccgtagtctg
     2221 gccccatgag gatggtaggc gtgaggctcg gatatccgag gaacgggtcc tcaccgtggc
     2281 gcccgatctc ctctttctcg agctcttcga ccgcgaccac gaaccgcgcg gccctggtta
     2341 cggggttgac cccgctcagc ggcatggcac cgtgcgccat cctcccgcgc accgtaacct
     2401 cgaccctgag cgcgcccttc tgctttacgc agagctggtt ctcttcgggc tcgcagatga
     2461 cggcggcgtc gacccccccg gcatggccgt tctcgatgaa cgtcttgatg cccgtcatca
     2521 tcccctcctc gtccacggga tgacagagta ccagggtacc ggggaaagag acaccggagt
     2581 cctttatggc acgcacggcg accagggcgg ccgcaaggtt ccccttggtg tcacaggcac
     2641 cgcgaccgta gaggcgtccg ccttcgcgct ctgccgcgaa aggagggtgt cgccactcct
     2701 gcgcattgcc ctccgtcacc acatccgtgt gcgcctcgaa gagcaacgtt ttgcccggac
     2761 gttcgccctc ccacacgacc cagacgttcg gcctgcctgg ggcaacatct tcggtcctcg
     2821 cttcgaagcc ggagtttcgc aggtagtcgg cgacgaaccc ggcgacgtcg ccctcgttcc
     2881 catcctcttc cccgggacgg tagacgcttg ggattcgcac gaggtctccc gcgaggcgca
     2941 cgagttcttc acggtcgatc ccggcgagta cgtcttcgag cacgctttcg aacacggccc
     3001 aagtttaacc gcctcacgtt ctccgggcat ggacctgcac ctccggggag gcagcgtgtt
     3061 gcgtgggcac ccacgctgcc ttatggttcg tgggatcttc ccgtcaagag aaccgagaag
     3121 gaggggcgtg tgaggagatc cagcgccacc ggtcgtccac ctgccggtct gcccctggcg
     3181 gacaacagta tctgtagaag ggcctgaacc ttgcgggtct cggaggcgat tggtagggtg
     3241 ctcgtggata acggcgtcgg ggcgtttttc ggcctggcgg ggagcggcaa cttcgcagtg
     3301 ctcaacgatt tgcacgaggc cggcgccgct ttctactcct ctaggcatga ggccggcgcg
     3361 gtcatgatgg ctgacggcta cgcccgcgcg agcggaaagc caggcgtcgc cagcgtccac
     3421 cagggccccg gcttcaccaa cgcgctcacc ggcctcgcgg aggccgcgaa ggcccggaca
     3481 ccgctcctct tgctcgccgc cgacataccg gccggaacgc tctggtcgaa cttcaaggtg
     3541 gaccagggct ccctcgccgc gacgctcggc gcgatccccg agcgcgtccg cacgcccgct
     3601 acggcggccg aggatacggc ccgcgccct
//
LOCUS       DBNGVD010000627         2214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate MAG05390
            SRR25158512_k127_3643564, whole genome shotgun sequence.
ACCESSION   DBNGVD010000627 DBNGVD010000000
VERSION     DBNGVD010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414798
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Bacillati; Actinomycetota; Rubrobacteria;
            Rubrobacterales; Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:24:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,328
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,276
            CDSs (with protein)               :: 3,276
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 5 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158512_k127_3643564"
                     /isolate="MAG05390"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..352)
                     /locus_tag="AC16CM_16630"
     CDS             complement(50..352)
                     /locus_tag="AC16CM_16630"
                     /inference="COORDINATES: protein motif:HMM:NF045463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6881 domain-containing protein"
                     /protein_id="HIR3141511.1"
                     /translation="MSQTYLRTEWIHDFQVEPYLFYHELDHERYATRKVEVFKDGRMI
                     RASAKVLERDPTALPDQPVPPLEELNTEDNVRVWQVTAQEFEEAWGAASGDRSPGC"
     gene            complement(349..1188)
                     /gene="whiA"
                     /locus_tag="AC16CM_16635"
     CDS             complement(349..1188)
                     /gene="whiA"
                     /locus_tag="AC16CM_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR3141512.1"
                     /translation="MRRQLAAGVATSSVGRYELAGLVDASGIWDEGGVTLRTPSAAVA
                     RSVVRLWRSEFGVDSRLSPIEPDRFGRTRYAVRTAGNGAIQAAEELKFARTSRAPAER
                     AGYLRGAFQGAGSVNRPGGRGGHHLEFVHREEEFIRRCAAFSRAPLKVVRRRGRWVAY
                     TKSADGVTTVLAQMGLSEAVLEYEARAVLGEAKANANRVTNFDAANAGRTATAAARQQ
                     RAFQNLDPENLPPALREMLELRIENPDASLTELARLGGVSRSATNHRLRRLVALSGEP
                     ERE"
     gene            complement(1190..2152)
                     /locus_tag="AC16CM_16640"
     CDS             complement(1190..2152)
                     /locus_tag="AC16CM_16640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR3141513.1"
                     /translation="MRSVEDLKVVAFGGGTGLPVLLRGLRDRGGDLTAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRRRLAEVFNYRFEAPGDLNDHSVGNIIIAALAD
                     MAGGFCEGVEQASQFLRIKGRVFPAAMESLTLVVRYADGTVTRGESVVHETGKQASEV
                     AVEPAGVPAPRGVIEAIEDADVIVLAPGSLFTSTIPALLGGGVREALEDFTGPVVYVA
                     NVMTQQGETGGFTVSDHVRAISKHVGPVVTDVLVHAGDLPPGTLARYEAEGATPVVVD
                     SGSLRKMGLHLTERDLLSSESSSGVRHDPGLLAREVCEAVLVRL"
BASE COUNT          358 a          771 c          717 g          368 t
ORIGIN      
        1 gttcattgtg cccatattcg ccgggttctt aacagaaggg aagagcttat cagcaacccg
       61 gcgatctgtc accggacgcc gcaccccacg cctcctcgaa ctcctgcgcg gtgacctgcc
      121 agacacgtac gttatcttcg gtgttcaact cttccagagg cggcacgggc tggtcgggaa
      181 gcgccgtggg atcacgctcc agaactttcg cactggccct tatcatcctg ccatccttga
      241 agacttctac cttcctggtt gcgtaccgct cgtggtcgag ttcgtggtag aagagatacg
      301 gttcgacctg gaagtcatgg atccactcgg tacggagata cgtctggctc attctctttc
      361 tggttcaccc gagagagcga cgagcctcct caaccgatgg ttggtggcgc tcctggagac
      421 gccgccgagt cgggctagct cggtgagcga ggcgtcgggg ttttcgatcc tgagctccaa
      481 catctcccgg agggcgggag ggaggttctc cgggtcgagg ttctggaagg ctctttgctg
      541 gcgggccgca gccgtggcgg tgcggccggc gttggcggcg tcgaagttgg tgacgcggtt
      601 ggcgtttgcc ttggcctccc cgagaaccgc cctggcctcg tactccagca cggcctcgct
      661 caggcccatc tgggcgagca cggtggtcac gccgtcggcg ctcttggtgt aggcgaccca
      721 ccgtccgcgc cggcgcacga ccttcaaggg cgcgcgggag aaagcggcgc atcgcctgat
      781 gaactcttcc tcgcggtgga cgaactccag gtggtggccc ccgcggccgc cgggccgatt
      841 cacggagccg gctccctgga aagcgccccg caggtagccg gcccgctcgg cgggggcgcg
      901 agaagtccgg gcgaacttca actcctcggc ggcctgtatc gcgccgttgc cggcagtacg
      961 caccgcgtaa cgggtccggc cgaaacggtc aggctctatg ggcgagagcc tcgagtcaac
     1021 cccgaactcc gagcgccaca gccgcacgac cgagcgggcg accgcggcgc tgggggtgcg
     1081 tagggttacg ccaccttcat cccagatacc agaagcatct accaggccgg cgagctcgta
     1141 acggccgacg gagctcgtcg cgaccccggc agcgagctgc cgacgcacct cagaggcgaa
     1201 cgaggacagc ctcgcacacc tccctcgcca gcagccccgg atcgtgcctg acaccactgc
     1261 tgctctccga agacaggaga tcgcgctccg tcaggtgcag gcccatcttc cgcaagctgc
     1321 cactgtcgac aacgaccggg gttgcaccct cggcctcgta gcgggcgagg gtgccgggcg
     1381 gcagatctcc cgcgtggacg agcacgtcgg tgacgaccgg cccgacgtgt ttcgagatcg
     1441 cccgcacgtg gtcggagacc gtgaagccgc ccgtctcgcc ctgctgggtc atcacgttgg
     1501 ccacgtaaac gaccgggccc gtgaagtcct ccagtgcctc cctcaccccg ccgccgagga
     1561 gggccgggat cgtgctggta aacaggcttc caggggcgag cacgatcacg tcggcgtctt
     1621 cgatggcctc tatgaccccc cgcggggccg gaactccagc tggctccacg gcgacctcgg
     1681 aggcctgttt tccggtctcg tgcaccacgg actcaccgcg cgtcacggtt ccgtcggcgt
     1741 agcgcaccac gagcgtaagg ctctccatcg cggccgggaa cacccggcct ttgatcctca
     1801 ggaactggga ggcctgctcc acaccctcgc agaacccccc tgccatgtca gcgagggcgg
     1861 cgatgatgat gttgccgacg ctgtggtcgt tcaggtcacc gggtgcctcg aagcggtagt
     1921 tgaagacctc ggccagcctg cggcgccccg cgagcgcgac caggcagttc ctgacgtcgc
     1981 cgggtggtgc cacgccaagc tcctggcgaa ggcgcccgct ggagcccccg tcgtcggcga
     2041 ccgtgacaac cgccgtaagg tcgccacccc tgtccctcag gcccctgagc aacacgggca
     2101 ggccggtgcc accgccgaac gctactacct tcaaatcctc gacgctacgc atgggccccg
     2161 agtataccgc cggccgaaaa cgcctaggcg tagagcacgg cccggtcgtg cggc
//
